I think that you will find that the oil contamination comes almost solely from the rotary backing pump, not from the DP, so a foreline trap between the DP and the RP will work very well. It will also be cheaper and much less trouble than a LN2 trap.
You may like to read Wil Bigelow's excellent book on EM vacuum systems.
cheers
rtch
Date sent: Fri, 30 Sep 2005 01:11:52 -0500 To: r.sims-at-auckland.ac.nz X-from: tylko-at-zuk.iz.uj.edu.pl Send reply to: tylko-at-zuk.iz.uj.edu.pl
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ucdstm-at-yahoo.com) from http://www.microscopy.com/MLFormMail.html on Saturday, October 1, 2005 at 17:42:11 ---------------------------------------------------------------------------
Email: ucdstm-at-yahoo.com Name: Christopher Fleming
Organization: University of California, Davis
Title-Subject: [Filtered] MListserver:
Question: What are a few suppliers of high quality PtIr STM tips?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (K.venner-at-ion.ucl.ac.uk) from http://www.microscopy.com/MLFormMail.html on Monday, October 3, 2005 at 03:20:55 ---------------------------------------------------------------------------
Question: The use of nose grease is news to me in the EM Lab, and will be filed away for future use. However, the use of nose grease is not confined to science; my flute teacher advocated applying it to the little finger on the right hand, to enable rapid and smooth key changes on the lower end of the instrument. Works a treat!
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (lazarcorp-at-cs.com) from http://www.microscopy.com/MLFormMail.html on Monday, October 3, 2005 at 07:20:41 ---------------------------------------------------------------------------
Email: lazarcorp-at-cs.com Name: Dennis Lazar
Title-Subject: [Filtered] MListserver:
Question: Is there a way to notify list members that I have a LFR Mk3 film recorder to donate to a university, school, etc. It is nearly new in original shipping carton. All software and hardware plus accessories. I do not want to join the list but can someone post this for me?
On the topic of getting films to float off of slides.
My advisor taught me to polish new slides directly out of the box with crumpled up Ross lens tissue, and to run the edge of a different dry slide (held at about a 45 degree angle) along the edges of the coated slide to separate the film from the glass when the slide is immersed in water. Scrape down from the top of each long edge, then twice on the bottom (from the middle to one side, then from the middle to the other). Breathe on the film before floating it off. The films almost always come off, even in class demos, with no hint of foundation makeup-contaminated nose grease - not a concern, I'm sure, for all members of the list ;)
You need to rub all the way out to the edges of the flat top and bottom of the slide with the lens tissue carefully (slide edges are sharp). I've always used Corning brand slides, so I don't know whether there's something special about them. I have no commercial interests in Ross lens tissue, Corning slides, or Clinique, except as a satisfied user.
Heather Owen
Dr. Heather A. Owen, Director Electron Microscope Laboratory Department of Biological Sciences University of Wisconsin - Milwaukee Lapham Hall 3209 N. Maryland Ave. Milwaukee, WI 53211 USA
Phone: (414)229-6816
==============================Original Headers============================== 10, 20 -- From owenha-at-csd.uwm.edu Mon Oct 3 10:14:39 2005 10, 20 -- Received: from batch3.csd.uwm.edu (batch3.csd.uwm.edu [129.89.169.226]) 10, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j93FEdd1001958 10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 3 Oct 2005 10:14:39 -0500 10, 20 -- Received: from alpha2.csd.uwm.edu (alpha2.csd.uwm.edu [129.89.7.202] (may be forged)) 10, 20 -- by batch3.csd.uwm.edu (8.12.10/8.12.6) with ESMTP id j93FEdum001540 10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 3 Oct 2005 10:14:39 -0500 (CDT) 10, 20 -- Received: from localhost (owenha-at-localhost) 10, 20 -- by alpha2.csd.uwm.edu (8.12.10/8.12.6) with SMTP id j93FEcVi006897 10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 3 Oct 2005 10:14:39 -0500 (CDT) 10, 20 -- X-Authentication-Warning: alpha2.csd.uwm.edu: owenha owned process doing -bs 10, 20 -- Date: Mon, 3 Oct 2005 10:14:38 -0500 (CDT) 10, 20 -- From: Heather A Owen {owenha-at-csd.uwm.edu} 10, 20 -- Reply-To: Heather A Owen {owenha-at-csd.uwm.edu} 10, 20 -- To: microscopy-at-microscopy.com 10, 20 -- Subject: Re: [Microscopy] viaWWW: preparing coated grids for TEM 10, 20 -- In-Reply-To: {200509301901.j8UJ1tvJ010658-at-ns.microscopy.com} 10, 20 -- Message-ID: {Pine.OSF.3.96.1051003092948.28791A-100000-at-alpha2.csd.uwm.edu} 10, 20 -- MIME-Version: 1.0 10, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
Victoria, I have access to the manuals that you requested and will contact you off-List. The SEM is located at the Univ. of Pennsylvania there is a chance that it may be de-commissioned but that is not certain. If it does, then the part you wish may be available also. Pat Connelly
vfink-at-shaw.ca wrote: Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (vfink-at-shaw.ca) from http://www.microscopy.com/MLFormMail.html on Wednesday, September 28, 2005 at 00:37:47 ------------------------------------------------ Email: vfink-at-shaw.ca Name: Victoria Fink Organization: N/A Title-Subject: [Filtered] MListserver: Looking for manuals & documents for Amray 1000A SEM
I am looking for the manuals, or copies of any documents ( electrical schemes, etc.) for Amray 1000A SEM? My friend bought used one. Also, he is trying to find, and interested to purchase the electron gun for this instrument. Thank you in advance for your kind advise, and any help, information regarding to this matter.
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==============================Original Headers============================== 5, 18 -- From stranen_connelly-at-yahoo.com Mon Oct 3 10:30:43 2005 5, 18 -- Received: from web34104.mail.mud.yahoo.com (web34104.mail.mud.yahoo.com [66.163.178.102]) 5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j93FUh0v010794 5, 18 -- for {microscopy-at-microscopy.com} ; Mon, 3 Oct 2005 10:30:43 -0500 5, 18 -- Received: (qmail 11474 invoked by uid 60001); 3 Oct 2005 15:30:43 -0000 5, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 18 -- s=s1024; d=yahoo.com; 5, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 5, 18 -- b=bvohGgCWrsKRTQ39U6KFD0Iu8N2joOMM1hnxGG6tn2RWQHW/+ErVWJMZSuW4koTkHPws/KqmndJfRoGH49NWig+cpgL5QbXrvy/ndxEN3N44BJn9WLDbvlzEg6Rnz27VffYCQEWWx0Ocw3GkkHy2hW4/SbKfcWLcAPpUuyCzY9w= ; 5, 18 -- Message-ID: {20051003153043.11472.qmail-at-web34104.mail.mud.yahoo.com} 5, 18 -- Received: from [68.44.176.215] by web34104.mail.mud.yahoo.com via HTTP; Mon, 03 Oct 2005 08:30:43 PDT 5, 18 -- Date: Mon, 3 Oct 2005 08:30:43 -0700 (PDT) 5, 18 -- From: Pat Conelly {stranen_connelly-at-yahoo.com} 5, 18 -- Subject: Looking for manuals & documents for Amray 1000A 5, 18 -- To: microscopy-at-microscopy.com 5, 18 -- MIME-Version: 1.0 5, 18 -- Content-Type: text/plain; charset=iso-8859-1 5, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I have used the Thermonox line of cover slips, and there is no problem using Ethanol or Acetone for dehydration or Propylene oxide and I have grown many types of cells on them and so far all have been very happy.
For face on cutting I always cut the bottom of the cell layer first, choosing an area that was more dense than is usually viewed at LM level - there are more cells/section and it is easy to see the cell membranes at TEM level if one needs to know which cell he is in.
To eliminate the plastic of the coverslip I trim so that I catch an edge of the boundry between the coverslip and the embedding epon with a razor blade or the corner of a glass knife and this popps off the plastic leaving a shiny surface ready to be sectioned. Next I align the diamond knife NEARLY parallel so that I do not section the whole face, just most of it. That way if I make a little mistake in judgement, there is still a lot of material to section and the sections show a variety of depths of the cells with some at the bottom surface while others show the gradual levels of middle to surface depending on the tilt of the block. Many serial sections can be picked up on the same grid if just the bottom edge of the block is sectioned for the sections will be very thin from top to bottom but can be as wide as one is comfortable with.
For cross section results one can use a sandwich type of embedding by putting 2 pieces of coverslips into a mold with the cell layers facing eachother. Trim into the coverslips on both sides with a glass knife (better control of depth) and the sections can be nearly two mm in length but very narrow. This is similar to a re-embedding technique that I presented at M&M '77 and is published in Hyatt's TEM books.
If you do have a Hayett - PLEASE make a correction for future reference...in working with TC cells grown in standard plastic petri dishes or flasks like Falcon, Costar, etc. several epon substitutes will melt the plastic especially when in the oven so cross out the word "Epon" and replace it with "LX-112 (Ladd) or similar substitute". Most of you already know about this but for beginners it can ruin an experiment if followed as written. At the time that I presented the paper I was still using the original epon from Shell and was unaware that the several substitutes had different properties until about 10 years ago when I needed to use the technique again and melted a few dishes!
Pat Connelly (unemployed in Pennsylvania)
--- TindallR-at-missouri.edu wrote: } One commonly used substrate is the Thermonox line of } cover slips,available from many suppliers. They come in various sizes and the cells can be grown on them, then the coverslips with cells fixed, dehydrated and embedded as usual. The section quite well, except for an unfortunate tendency for sections not to detach completely in the boat and to pull the next section back over the edge of the knife. This can be remedied by making a pointy block face or by using a razor blade to make a line just beneath the top of the block face so the section detaches just before the end of the cutting stroke.
The cover slips can be cut into pieces small enough to embed in ordinary capsules and are normally used to do cross-sections through cell layers, as opposed to "top down" sections. With care and creativity you should also be able to get other orientations, but it would be much trickier.
} I'm not 100% sure of their compatibility with PO, but I don't believe it's a problem. They stand up fine to EPON and other resins we use.
An alternative is to grow the cells on any old cover slips, run them through the fixation, dehydration, and infiltration steps, then place them cell side down on top of resin filled (with a resin meniscus slightly up over the top) embedding capsules. Polymerize them as usual, taking care that they are level so the cover slips don't slide off. After everything is mostly hard, immerse the coverslip/capsule assembly in liquid nitrogen, then snap off the coverslip from the capsule. If all goes well, and it usually does, the cells with transfer to the resin in the capsule and form the top layer of the block. Identify your area under a light microscope, trim away the rest (careful!), then start taking sections. Remember that there is only one cell layer----if you cut through it and discard it by mistake, it's gone.
} Randy Tindall } EM Specialist } Electron Microscopy Core Facility } W122 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu
} -----Original Message----- } X-from: Ralph.Common-at-hc.msu.edu } Sent: Thursday, September 29, 2005 } Subject: [Microscopy] substrate for cell culture } } A client needs to have cell cultures of endothelial } cells embedded for TEM. I would be grateful to hear (off line) specific recommendations } for a substrate for growing the cells. The } substrate will have to } withstand ethanol and propylene oxide, and have good } sectioning } properties when used with an Epon type resin. I } would also appreciate recommendations for fixation. } } Ralph Common } Division of Human Pathology } Michigan State University
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==============================Original Headers============================== 13, 20 -- From stranen_connelly-at-yahoo.com Mon Oct 3 11:30:26 2005 13, 20 -- Received: from web34101.mail.mud.yahoo.com (web34101.mail.mud.yahoo.com [66.163.178.99]) 13, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j93GUQPe020491 13, 20 -- for {microscopy-at-microscopy.com} ; Mon, 3 Oct 2005 11:30:26 -0500 13, 20 -- Received: (qmail 8628 invoked by uid 60001); 3 Oct 2005 16:30:25 -0000 13, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 13, 20 -- s=s1024; d=yahoo.com; 13, 20 -- h=Message-ID:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 13, 20 -- b=N37GT714/gvGk2CMRfx0ZSkpva7HS5cyVnnC22DwdSmyT1an1s7JE9/9uznwVDeDAaKZMJqyg+vEw9qcZMk0j/5H2S/5I7KA1FoCsRDULc5mCAgY9HrNm82zS+HFr/qVfencC5ugrXmK3TyKsu0GR+uIUNNRQOs7tvYuNX0EAaM= ; 13, 20 -- Message-ID: {20051003163025.8626.qmail-at-web34101.mail.mud.yahoo.com} 13, 20 -- Received: from [68.44.176.215] by web34101.mail.mud.yahoo.com via HTTP; Mon, 03 Oct 2005 09:30:25 PDT 13, 20 -- Date: Mon, 3 Oct 2005 09:30:25 -0700 (PDT) 13, 20 -- From: Pat Conelly {stranen_connelly-at-yahoo.com} 13, 20 -- Subject: Re: [Microscopy] RE: substrate for cell culture 13, 20 -- To: TindallR-at-missouri.edu 13, 20 -- Cc: microscopy-at-microscopy.com 13, 20 -- In-Reply-To: {200509292152.j8TLqcaA010640-at-ns.microscopy.com} 13, 20 -- MIME-Version: 1.0 13, 20 -- Content-Type: text/plain; charset=iso-8859-1 13, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
On Sep 30, 2005, at 12:00 PM, paul r hazelton wrote:
} 1. i've always used dichloroethane for formvar. what advantage would } there be to chloroform - how well does work? } } 2. ok, i gotta plead ignorance. for delicacy perhaps we should use } apiezon for the example and leave 'nose grease' to our fertile } imaginations. but in 35 years i've never heard of using apiezon to } help } get the formvar off the slide for coated grids. i'm deeply intrigued. } enlighten me. enlighten the rest of us. it could be a good trick to } know. } Dear Paul, Our lab in Albany had a recipe that called for dissolving formvar in equal parts chloroform and acetone. I have also used the pre-made solution of formvar in dichloroethane, and I haven't found any particular difference. I don't know which of the two chloro-carbons is more stable--I suspect DCE--but that would be the preferred solvent. I tried two methods with apiazon, and had about equal success. I should say that I was making holey formvar to end up with holey carbon films, and I was having absolutely no luck getting the films to separate from the glass slides until I tried the apiazon. The lab's procedure called for taking pre-cleaned slides and rinsing them in ethanol, then wiping them dry. They were then made more-or-less controllably less clean by applying a thin coat of grease, but when the oil from my skin proved to be deficient, and that from other members of the lab worked, I decided to try something more well-defined. One trick for aligning the holes in holey carbon is to apply the grease to the slide, then rub with one's finger and thumb along the long direction of the slide. The ridged residual grease will cause the holes (made by glycerol droplets) to line up along the ridges. When I first tried apiazon, I just used the same technique as with skin oil, using as small an amount of apiazon as I could. Later I thought to make the process even more well-defined by disolving the apiazon, and a relatively-high-boiling-point petroleum ether (hexanes or heptanes most likely) proved to be a good solvent. For this procedure, I dissolved a measured amount of apiazon in 50 ml of solvent, then dipped the slide and let it drain in the same manner as used for dipping in formvar solution. In order to try to avoid any residual grease remaining on the underside of the formvar, I floated the film off with a dilute, warm detergent solution--0.25 g Alconox in 1 L DDH2O. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 5, 27 -- From tivol-at-caltech.edu Mon Oct 3 11:59:24 2005 5, 27 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j93GxOqx029481 5, 27 -- for {microscopy-at-msa.microscopy.com} ; Mon, 3 Oct 2005 11:59:24 -0500 5, 27 -- Received: from localhost (fire-dog [192.168.1.4]) 5, 27 -- by fire-ox-postvirus (Postfix) with ESMTP id CB8F33527C 5, 27 -- for {microscopy-at-msa.microscopy.com} ; Mon, 3 Oct 2005 09:59:21 -0700 (PDT) 5, 27 -- Received: from earth-ox ([192.168.1.9]) 5, 27 -- by fire-dog (MailMonitor for SMTP v1.2.2 ) ; 5, 27 -- Mon, 3 Oct 2005 09:59:20 -0700 (PDT) 5, 27 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 5, 27 -- by earth-ox.its.caltech.edu (Postfix) with ESMTP id D7868109B7D 5, 27 -- for {microscopy-at-msa.microscopy.com} ; Mon, 3 Oct 2005 09:59:18 -0700 (PDT) 5, 27 -- Mime-Version: 1.0 (Apple Message framework v623) 5, 27 -- In-Reply-To: {433D8B3C.8346B7E6-at-umanitoba.ca} 5, 27 -- References: {200509301819.j8UIJHo7026583-at-ns.microscopy.com} {433D8B3C.8346B7E6-at-umanitoba.ca} 5, 27 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 5, 27 -- Message-Id: {abf1f26358bf503e31e17252e4a0ce87-at-caltech.edu} 5, 27 -- Content-Transfer-Encoding: 7bit 5, 27 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 27 -- Subject: Re: [Microscopy] Re: viaWWW: preparing coated grids for TEM 5, 27 -- Date: Mon, 3 Oct 2005 10:02:39 -0700 5, 27 -- To: microscopy-at-msa.microscopy.com 5, 27 -- X-Mailer: Apple Mail (2.623) 5, 27 -- X-Spam-Status: No, hits=-4.52 tagged_above=-10000 required=5 5, 27 -- tests=[ALL_TRUSTED=-2.82, CIT_FROM_ADDR=-0.7, CIT_NET_FROM=-1] 5, 27 -- X-Spam-Level: ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (connie.a.cummings-at-gsk.com) from http://www.microscopy.com/MLFormMail.html on Monday, October 3, 2005 at 10:43:15 ---------------------------------------------------------------------------
Question: Good day, I am in need of some information from those folks out there that have a JEOL 1010 microscope? Are you happy with it? Has it had many problems and if so what type of problems? I appreciate any feedback. Thanks so much. Connie
We are trying to image the hydrophilic/hydrophobic bulk phase separation behavior of a series of partially fluorinated disulfonated poly(arylene ether sulfone) random copolymers using TEM. The copolymers are completely amorphous and the only difference between the hydrophilic and hydrophobic sections of their backbone is that the hydrophilic section possesses two sulfonic acid groups, each one covalently bonded to one of two arylene rings which flank a sulfone group. A membrane solvent-cast from one of these copolymers was quantitatively titrated with cesium hydroxide in an attempt to increase the difference in electron density between the hydrophobic and hydrophilic domains. Despite efforts to microtome samples as thin as possible (~50 nm), little to no contrast was observed in the micrographs of these samples imaged by TEM at 100kV. We would appreciate any suggestions for alternative staining techniques for these copolymers.
Stephen McCartney Senior Research Associate Macromolecules and Interfaces Institute 2108 Hahn Hall Va Tech Blacksburg, VA 24061 540-231-9765 - phone 540-231-8517 - FAX
==============================Original Headers============================== 4, 21 -- From stmccart-at-vt.edu Mon Oct 3 13:02:00 2005 4, 21 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu [198.82.162.213]) 4, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j93I20FT015005 4, 21 -- for {microscopy-at-microscopy.com} ; Mon, 3 Oct 2005 13:02:00 -0500 4, 21 -- Received: from steiner.cc.vt.edu (IDENT:mirapoint-at-evil-steiner.cc.vt.edu [10.1.1.14]) 4, 21 -- by lennier.cc.vt.edu (8.12.11/8.12.11) with ESMTP id j93I20S1011544 4, 21 -- for {microscopy-at-microscopy.com} ; Mon, 3 Oct 2005 14:02:00 -0400 4, 21 -- Received: from mri-system.vt.edu (h80ada7f8.dhcp.vt.edu [128.173.167.248]) 4, 21 -- by steiner.cc.vt.edu (MOS 3.6.4-CR) 4, 21 -- with ESMTP id EBP67866; 4, 21 -- Mon, 3 Oct 2005 14:01:56 -0400 (EDT) 4, 21 -- Message-Id: {5.2.1.1.0.20051003135643.040e1f70-at-pop.vt.edu} 4, 21 -- X-Sender: stmccart-at-pop.vt.edu 4, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 4, 21 -- Date: Mon, 03 Oct 2005 14:01:58 -0400 4, 21 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 4, 21 -- From: Stephen McCartney {stmccart-at-vt.edu} 4, 21 -- Subject: TEM of partially fluorinated disulfonated poly(arylene ether 4, 21 -- sulfone) random copolymers 4, 21 -- Mime-Version: 1.0 4, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
We need an American Optical Model 27K Stereo Scope with an 8x objective. If we can't get the full microscope then we can take just the 8x objective. If any knows where we can get one please let me know.
Thank you,
Ron Peck
Ladd Research 83 Holly Court Williston, VT 05495
On-line Catalog: http://www.laddresearch.com
tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US) fax: 1-802-660-8859 e-mail: ladres-at-att.net
==============================Original Headers============================== 9, 23 -- From ladres-at-worldnet.att.net Mon Oct 3 15:13:02 2005 9, 23 -- Received: from mtiwmhc13.worldnet.att.net (mtiwmhc13.worldnet.att.net [204.127.131.117]) 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j93KD20d025831 9, 23 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 3 Oct 2005 15:13:02 -0500 9, 23 -- Received: from TheDuke (86.syracuse-09-10rs.ny.dial-access.att.net[12.75.109.86]) 9, 23 -- by worldnet.att.net (mtiwmhc13) with SMTP 9, 23 -- id {2005100320130111300ev627e} ; Mon, 3 Oct 2005 20:13:01 +0000 9, 23 -- Message-ID: {003301c5c856$dd943ef0$7e81fea9-at-TheDuke} 9, 23 -- Reply-To: "Ladd Research" {ladres-at-att.net} 9, 23 -- From: "Ladd Research" {ladres-at-worldnet.att.net} 9, 23 -- To: "Listserver" {Microscopy-at-msa.microscopy.com} 9, 23 -- Subject: need a microscope 9, 23 -- Date: Mon, 3 Oct 2005 16:13:04 -0400 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- format=flowed; 9, 23 -- charset="iso-8859-1"; 9, 23 -- reply-type=original 9, 23 -- Content-Transfer-Encoding: 7bit 9, 23 -- X-Priority: 3 9, 23 -- X-MSMail-Priority: Normal 9, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 9, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
I have a question about one of the steps in CPD using ethanol and CO2. We have both a Denton DCP-1 and a Bomer 900EX. I was taught that I should never let the sample exposed to air after passing the higher alcohol concentrations e.g., 75% and above. When transferring the dehydrated sample from the ethanol into the CPD chamber, I will fill the chamber with enough ethanol to submerge the prep and then transfer the sample basket/container quickly into the CPD chamber. However, I have come across quite a few protocols that either do not specify this or simply fill the chamber with CO2 "snow".
So my question is, should I fill the chamber with ethanol? Am I being overly cautious or have I missed anything critical (no puns intended!)? Can this be sample specific e.g., smaller or delicate samples may be more prone to surface tension disruption? Any advice and comments are much appreciated.
-- Pang (Wai Pang Chan, wpchan-at-u.washington.edu)
==============================Original Headers============================== 4, 19 -- From wpchan-at-u.washington.edu Mon Oct 3 16:03:54 2005 4, 19 -- Received: from mxout7.cac.washington.edu (mxout7.cac.washington.edu [140.142.32.178]) 4, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j93L3rbj002708 4, 19 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 3 Oct 2005 16:03:53 -0500 4, 19 -- Received: from homer23.u.washington.edu (homer23.u.washington.edu [140.142.12.141]) 4, 19 -- by mxout7.cac.washington.edu (8.13.4+UW05.04/8.13.4+UW05.09) with ESMTP id j93L3qZ5014746 4, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 19 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 3 Oct 2005 14:03:52 -0700 4, 19 -- Received: from localhost (wpchan-at-localhost) 4, 19 -- by homer23.u.washington.edu (8.13.4+UW05.04/8.13.4+Submit) with ESMTP id j93L3qXL002981 4, 19 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 3 Oct 2005 14:03:52 -0700 4, 19 -- Date: Mon, 3 Oct 2005 14:03:52 -0700 (PDT) 4, 19 -- From: "W. Chan" {wpchan-at-u.washington.edu} 4, 19 -- To: Microscopy-at-msa.microscopy.com 4, 19 -- Subject: CPD 4, 19 -- Message-ID: {Pine.LNX.4.63a.0510031323190.26242-at-homer23.u.washington.edu} 4, 19 -- MIME-Version: 1.0 4, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 4, 19 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
Anybody have a 23 year old Hitachi X650 SEM standing around gathering dust? We, in Cape Town, South Africa, are in desperate need of spares. I would be really glad if you could help.
Thanks
Basil
Dr. Basil Julies Director Electron Microscope Unit Physics Department University of the Western Cape Private Bag X17 Bellville 7535 Tel : (27)(21) 959 2327 or 959 3458 Fax : (27)(21) 959 1335 or 959 3474
==============================Original Headers============================== 7, 22 -- From bjulies-at-uwc.ac.za Tue Oct 4 03:59:49 2005 7, 22 -- Received: from uwcunx.uwc.ac.za (uwcunx.uwc.ac.za [196.11.235.8]) 7, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j948xlsP027742 7, 22 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 03:59:48 -0500 7, 22 -- Received: (qmail 329 invoked by uid 1005); 4 Oct 2005 07:41:41 -0000 7, 22 -- Received: from bjulies-at-uwc.ac.za by uwcunx by uid 1002 with qmail-scanner-1.22 7, 22 -- (sweep: 2.10/3.65. Clear:RC:1(192.102.9.71):. 7, 22 -- Processed in 0.120792 secs); 04 Oct 2005 07:41:41 -0000 7, 22 -- Received: from itsnw.uwc.ac.za (HELO Services-02.uwc.ac.za) (192.102.9.71) 7, 22 -- by uwcunx.uwc.ac.za with SMTP; 4 Oct 2005 07:41:41 -0000 7, 22 -- Received: from UWC-MTA by Services-02.uwc.ac.za 7, 22 -- with Novell_GroupWise; Tue, 04 Oct 2005 11:02:42 +0200 7, 22 -- Message-Id: {s3426152.048-at-Services-02.uwc.ac.za} 7, 22 -- X-Mailer: Novell GroupWise Internet Agent 6.5.2 7, 22 -- Date: Tue, 04 Oct 2005 11:02:13 +0200 7, 22 -- From: "Basil Julies" {bjulies-at-uwc.ac.za} 7, 22 -- To: {microscopy-at-microscopy.com} 7, 22 -- Subject: 23 year old Hitachi X650 7, 22 -- Mime-Version: 1.0 7, 22 -- Content-Type: text/plain; charset=US-ASCII 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- Content-Disposition: inline ==============================End of - Headers==============================
I don't wish to turn this into a nose grease forum, but I did come across nose grease as a suitable emergency photographic film scratch remover. I believe it was an old journalist/photographer trick for those times when small scratches needed to be reduced in the darkroom.
I have tried this and it does work, but I would only ever use it for fine scratches on the back of film (not the emulsion side). If it's a valuable negative it might be safer to use something more proprietary.
I wonder if any of the e.m. supply companies has considered putting artificial nose grease onto their catalogues.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: K.venner-at-ion.ucl.ac.uk
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cornheadorama-at-hotmail.com) from http://www.microscopy.com/MLFormMail.html on Monday, October 3, 2005 at 20:51:14 ---------------------------------------------------------------------------
Email: cornheadorama-at-hotmail.com Name: Jeff Miller
Title-Subject: [Filtered] MListserver: High Vacuum Sealing Wax?
Question: Can anyone recommend some low vapor pressure sealing or adhesive waxes or putties for semi-permanent sealing of high vacuum leaks, and maybe to seal mis-matched flanges under compression: that kind of thing. Probably a pair of waxes with melting points of about 60C and 100C or more would help.
Obviously they should have good adhesion properties, preferrably at low temps as well. I'd expect that a small amount of flexibility would be helpful. Best if it has no colorant or secret ingredients.
I'm thinking some of the materials used in mouting specimens might work.
I haven't tried any waxes yet. I've checked specs on a few Apiezon waxes but they seem to have higher vapor pressures than their greases.
Vacseal seems too permanent for most of what I had in mind.
So far I've tried Duxseal, but it has an odor which I can't imagine is good for hi-vac. And sure enough it will cavitate and spring a leak: if you apply it, squeeze tight, and "remove" it you will often get a very good seal that leaks again a few hours later.
Critoseal has no odor and is mostly PVC, I'll be trying that and probably analyzing it for weight loss under vacuum soon. I'm wondering if there are some plumber's putties to consider, there might be some gimmicky teflon putty that could be just the ticket. I was recently gifted 1 oz. of amorphous Teflon powder, which I might try mixing with some heavy Apiezon greases.
The old remedy I recall from the Dark Ages is: "glyptal". It was originally marketed (by GE?) as an anti-corona goop , but soon thereafter someone discovered it had surprisingly good vacuum sealing properties as well.
Does anyone know if it's still available?
--Mike Young mike.young-at-yale.edu
cornheadorama-at-hotmail.com wrote:
} } } Title-Subject: [Filtered] MListserver: High Vacuum Sealing Wax? } } Question: Can anyone recommend some low vapor pressure sealing or adhesive waxes or putties for semi-permanent sealing of high vacuum leaks, and maybe to seal mis-matched flanges under compression: that kind of thing. Probably a pair of waxes with melting points of about 60C and 100C or more would help. } } {snip} } }
==============================Original Headers============================== 6, 22 -- From mike.young-at-yale.edu Tue Oct 4 08:28:18 2005 6, 22 -- Received: from pantheon-po06.its.yale.edu (pantheon-po06.its.yale.edu [130.132.50.36]) 6, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j94DSHxE023997 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 08:28:17 -0500 6, 22 -- Received: from [128.36.108.153] (mpy2bc511.eng.yale.edu [128.36.108.153]) 6, 22 -- (authenticated bits=0) 6, 22 -- by pantheon-po06.its.yale.edu (8.12.11/8.12.11) with ESMTP id j94DSHPw017472 6, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 09:28:17 -0400 6, 22 -- Message-ID: {43428375.4020203-at-yale.edu} 6, 22 -- Date: Tue, 04 Oct 2005 09:28:21 -0400 6, 22 -- From: Mike Young {mike.young-at-yale.edu} 6, 22 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 6, 22 -- X-Accept-Language: en-us, en 6, 22 -- MIME-Version: 1.0 6, 22 -- To: microscopy list {microscopy-at-microscopy.com} 6, 22 -- Subject: Re: [Microscopy] viaWWW: High Vacuum Sealing Wax? 6, 22 -- References: {200510041305.j94D57ZL023447-at-ns.microscopy.com} 6, 22 -- In-Reply-To: {200510041305.j94D57ZL023447-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-YaleITSMailFilter: Version 1.2b (attachment(s) not renamed) ==============================End of - Headers==============================
mike.young-at-yale.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } The old remedy I recall from the Dark Ages is: "glyptal". It was } originally marketed (by GE?) as an anti-corona goop , but soon } thereafter someone discovered it had surprisingly good vacuum sealing } properties as well. } } Does anyone know if it's still available? } } --Mike Young } mike.young-at-yale.edu } } cornheadorama-at-hotmail.com wrote: } } } } } } Title-Subject: [Filtered] MListserver: High Vacuum Sealing Wax? } } } } Question: Can anyone recommend some low vapor pressure sealing or adhesive waxes or putties for semi-permanent sealing of high vacuum leaks, and maybe to seal mis-matched flanges under compression: that kind of thing. Probably a pair of waxes with melting points of about 60C and 100C or more would help. } } } } {snip} } } } } } } } } ==============================Original Headers============================== } 6, 22 -- From mike.young-at-yale.edu Tue Oct 4 08:28:18 2005 } 6, 22 -- Received: from pantheon-po06.its.yale.edu (pantheon-po06.its.yale.edu [130.132.50.36]) } 6, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j94DSHxE023997 } 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 08:28:17 -0500 } 6, 22 -- Received: from [128.36.108.153] (mpy2bc511.eng.yale.edu [128.36.108.153]) } 6, 22 -- (authenticated bits=0) } 6, 22 -- by pantheon-po06.its.yale.edu (8.12.11/8.12.11) with ESMTP id j94DSHPw017472 } 6, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 09:28:17 -0400 } 6, 22 -- Message-ID: {43428375.4020203-at-yale.edu} } 6, 22 -- Date: Tue, 04 Oct 2005 09:28:21 -0400 } 6, 22 -- From: Mike Young {mike.young-at-yale.edu} } 6, 22 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) } 6, 22 -- X-Accept-Language: en-us, en } 6, 22 -- MIME-Version: 1.0 } 6, 22 -- To: microscopy list {microscopy-at-microscopy.com} } 6, 22 -- Subject: Re: [Microscopy] viaWWW: High Vacuum Sealing Wax? } 6, 22 -- References: {200510041305.j94D57ZL023447-at-ns.microscopy.com} } 6, 22 -- In-Reply-To: {200510041305.j94D57ZL023447-at-ns.microscopy.com} } 6, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 6, 22 -- Content-Transfer-Encoding: 7bit } 6, 22 -- X-YaleITSMailFilter: Version 1.2b (attachment(s) not renamed) } ==============================End of - Headers============================== }
--
Andy Buckley
AB78-at-ESC.CAM.AC.UK DEPARTMENT OF EARTH SCIENCES UNIVERSITY OF CAMBRIDGE Tel +44 1223 333469 DOWNING STREET +44 1223 333400 CAMBRIDGE FAX +44 1223 333450 CB2 3EQ
Manager of XRD discussion list: http://www.jiscmail.ac.uk/lists/xrd.html
==============================Original Headers============================== 7, 21 -- From ab78-at-esc.cam.ac.uk Tue Oct 4 08:41:10 2005 7, 21 -- Received: from rock.esc.cam.ac.uk (rock.esc.cam.ac.uk [131.111.41.250]) 7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j94Df9BV032631 7, 21 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 08:41:10 -0500 7, 21 -- Received: from andyb.esc.cam.ac.uk ([192.168.17.155]) 7, 21 -- by rock.esc.cam.ac.uk with esmtp (Exim 4.34) 7, 21 -- id 1EMn2Q-00013e-Ig 7, 21 -- for microscopy-at-microscopy.com; Tue, 04 Oct 2005 14:41:06 +0100 7, 21 -- Message-ID: {4342857B.90400-at-esc.cam.ac.uk} 7, 21 -- Date: Tue, 04 Oct 2005 14:36:59 +0100 7, 21 -- From: Andy Buckley {ab78-at-esc.cam.ac.uk} 7, 21 -- Reply-To: ab78-at-esc.cam.ac.uk 7, 21 -- User-Agent: Mozilla Thunderbird 1.0 (Windows/20041206) 7, 21 -- X-Accept-Language: en-us, en 7, 21 -- MIME-Version: 1.0 7, 21 -- To: microscopy-at-microscopy.com 7, 21 -- Subject: Re: [Microscopy] glyptal... Re: viaWWW: High Vacuum Sealing Wax? 7, 21 -- References: {200510041332.j94DWI5f030329-at-ns.microscopy.com} 7, 21 -- In-Reply-To: {200510041332.j94DWI5f030329-at-ns.microscopy.com} 7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I was re-reading some catalogues of exhibits of Van Leeuwenhoek microscopes, and I was struck, as usual, by the description of the glass lenses that he made for his observations. These descriptions reminded me of a comment that I overheard in passing at a recent M&M meeting that Van Leeuwenhoek also made (or used) lenses made of droplets of water. I know that he made a "water microscope", but I always understood this to mean that the sample was liquid, contained in a glass tube. Can anyone provide verification of the use of water as a lens?
Joel
Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 8, 19 -- From jbs-at-temple.edu Tue Oct 4 09:40:00 2005 8, 19 -- Received: from imp1.temple.edu (imp1.ocis.temple.edu [155.247.166.81]) 8, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j94Ee0iU010030 8, 19 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 09:40:00 -0500 8, 19 -- Received: from JBS (jbs.bio.temple.edu [155.247.98.40]) 8, 19 -- by imp1.temple.edu (8.12.3/8.11.3/SuSE Linux 8.11.1-0.5) with ESMTP id j94Ee05w016346 8, 19 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 10:40:00 -0400 8, 19 -- From: "Joel Sheffield" {jbs-at-temple.edu} 8, 19 -- To: microscopy-at-microscopy.com 8, 19 -- Date: Tue, 04 Oct 2005 10:37:58 -0400 8, 19 -- MIME-Version: 1.0 8, 19 -- Subject: History - Van Leeuwenhoek Lenses 8, 19 -- Reply-to: jbs-at-temple.edu 8, 19 -- Message-ID: {43425B86.4354.4BEC1FC4-at-localhost} 8, 19 -- Priority: normal 8, 19 -- X-mailer: Pegasus Mail for Windows (4.21c) 8, 19 -- Content-type: text/plain; charset=US-ASCII 8, 19 -- Content-transfer-encoding: 7BIT 8, 19 -- Content-description: Mail message body ==============================End of - Headers==============================
I haven't made a water drop microscope, but I have been making Leeuwenhoek glass ball microscope replicas. I haven't yet honed my lensmaking skills to resolve bacilli, spirilla, cocci, like he did, but diatoms look awesome through it! I just twirl a glass thread in a flame to make the spheres, but I don't think I have the patience to do the polishing that he did. I may cheat and buy one of the Edmund Optics glass ball lenses (~$20).
Paul Grover
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi all,
I was re-reading some catalogues of exhibits of Van Leeuwenhoek microscopes, and I was struck, as usual, by the description of the glass lenses that he made for his observations. These descriptions reminded me of a comment that I overheard in passing at a recent M&M meeting that Van Leeuwenhoek also made (or used) lenses made of droplets of water. I know that he made a "water microscope", but I always understood this to mean that the sample was liquid, contained in a glass tube. Can anyone provide verification of the use of water as a lens?
Joel
Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 18, 24 -- From pgrover-at-bilbo.bio.purdue.edu Tue Oct 4 10:21:56 2005 18, 24 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 18, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j94FLtRV019266 18, 24 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 10:21:55 -0500 18, 24 -- Received: from paklabpgrover (dhcp155-241.bio.purdue.edu [128.210.155.241]) 18, 24 -- by mailhub129.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id j94FLsWS009780 18, 24 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 10:21:55 -0500 18, 24 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu} 18, 24 -- To: {microscopy-at-microscopy.com} 18, 24 -- Subject: water lens van Leeuwenhoek microscopes 18, 24 -- Date: Tue, 4 Oct 2005 10:21:55 -0500 18, 24 -- Message-ID: {000001c5c8f7$5a9d9040$f19bd280-at-paklabpgrover} 18, 24 -- MIME-Version: 1.0 18, 24 -- Content-Type: text/plain; 18, 24 -- charset="us-ascii" 18, 24 -- X-Priority: 3 (Normal) 18, 24 -- X-MSMail-Priority: Normal 18, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.4510 18, 24 -- Importance: Normal 18, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 18, 24 -- X-PMX-Version: 4.7.1.128075 18, 24 -- X-PerlMx-Virus-Scanned: Yes 18, 24 -- Content-Transfer-Encoding: 8bit 18, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j94FLtRV019266 ==============================End of - Headers==============================
For some reason, if you just click on the url, the last part of it doesn't copy, and you won't get all the way to the web page. You may have to cut 'n paste the last part, or type it in, but it's worth the trouble!
I haven't made a water drop microscope, but I have been making Leeuwenhoek glass ball microscope replicas. I haven't yet honed my lensmaking skills to resolve bacilli, spirilla, cocci, like he did, but diatoms look awesome through it! I just twirl a glass thread in a flame to make the spheres, but I don't think I have the patience to do the polishing that he did. I may cheat and buy one of the Edmund Optics glass ball lenses (~$20).
------------------------------------------------------------------------ May your trails be crooked, winding, lonesome, dangerous, leading to the most amazing view.
- Edward Abbey
==============================Original Headers============================== 10, 24 -- From pgrover-at-bilbo.bio.purdue.edu Tue Oct 4 10:36:50 2005 10, 24 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 10, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j94FanLt027960 10, 24 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 10:36:49 -0500 10, 24 -- Received: from paklabpgrover (dhcp155-241.bio.purdue.edu [128.210.155.241]) 10, 24 -- by mailhub129.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id j94FanN4011651 10, 24 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 10:36:49 -0500 10, 24 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu} 10, 24 -- To: {microscopy-at-microscopy.com} 10, 24 -- Subject: re: water drop microscope - p.s. 10, 24 -- Date: Tue, 4 Oct 2005 10:36:49 -0500 10, 24 -- Message-ID: {000001c5c8f9$6ff70a00$f19bd280-at-paklabpgrover} 10, 24 -- MIME-Version: 1.0 10, 24 -- Content-Type: text/plain; 10, 24 -- charset="us-ascii" 10, 24 -- X-Priority: 3 (Normal) 10, 24 -- X-MSMail-Priority: Normal 10, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.4510 10, 24 -- Importance: Normal 10, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 10, 24 -- X-PMX-Version: 4.7.1.128075 10, 24 -- X-PerlMx-Virus-Scanned: Yes 10, 24 -- Content-Transfer-Encoding: 8bit 10, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j94FanLt027960 ==============================End of - Headers==============================
Do you mean glyptal red enamel ? - the automotive stuff that is used to seal glass coverslips to slides like high performance nail varnish (for cleared insect bodies etc...). It's a hard [cellulose derived polyester alkyd resin] paint you see on dynamo's & other electric machinery and some engines (and it seals up pores in engine blocks). Glyptal was made by General Electric (who coined the name in the 20's). If so try:
http://onlinesaleshop.biz/eastwood-product-2591.html (or the link I notice has just been given )
http://www.glyptal.com/ is down at the moment for some reason but they do make other types (I'm sure black at least).
I'm sure I've have seen it somewhere as a clear varnish that may suit better, as a re-inforcement for cloth mouldings. I think glyptal paint is normally baked in a curing oven when on metal (which makes it rather permanent should things go wrong).
Sam's laser facts suggests using Glyptal red for vacuum sealing : "Anyhow, once the whole affair was drenched in Red Glyptal, leaks really weren't an issue!" : http://weber.ucg.ie/mirrors/www.repairfaq.org/sam/lasercon.htm (search for Glyptal) so I suppose it must be OK.
Originally clear Glyptal resin was used similarly to shellac (e.g. as a hard coating/glue). It ages badly though going red tinged over the years (eg. on fossils). You can use acetone to remove Glyptal when used as a sealant on slides (but its not very easy), but I'm not sure it will be at all easy if bake cured onto metal.
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
Thank you very much. I enjoyed the references. It's an amusing idea to use the biological lens as a microscope lens. So, did Van Leeuwenhoek himself use one of these?
When I entered this, the web site generated the full URL. Odd.
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } For some reason, if you just click on the url, the last part of it } doesn't copy, and you won't get all the way to the web page. You may } have to cut 'n paste the last part, or type it in, but it's worth the } trouble! } } } Joel, } } Check out } } http://www.microscopy-uk.net/mag/indexmag.html?http://www.microscopy-u } k.net/ mag/art98/watermic.html } } I haven't made a water drop microscope, but I have been making } Leeuwenhoek glass ball microscope replicas. I haven't yet honed my } lensmaking skills to resolve bacilli, spirilla, cocci, like he did, } but diatoms look awesome through it! I just twirl a glass thread in a } flame to make the spheres, but I don't think I have the patience to do } the polishing that he did. I may cheat and buy one of the Edmund } Optics glass ball lenses (~$20). } } ---------------------------------------------------------------------- } -- May your trails be crooked, winding, lonesome, dangerous, leading } to the most amazing view. } } - Edward Abbey } } } } } ==============================Original } Headers============================== 10, 24 -- From } pgrover-at-bilbo.bio.purdue.edu Tue Oct 4 10:36:50 2005 10, 24 -- } Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu } [128.210.5.129]) 10, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with } ESMTP id j94FanLt027960 10, 24 -- for {microscopy-at-microscopy.com} ; } Tue, 4 Oct 2005 10:36:49 -0500 10, 24 -- Received: from paklabpgrover } (dhcp155-241.bio.purdue.edu [128.210.155.241]) 10, 24 -- by } mailhub129.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id } j94FanN4011651 10, 24 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct } 2005 10:36:49 -0500 10, 24 -- From: "pgrover" } {pgrover-at-bilbo.bio.purdue.edu} 10, 24 -- To: } {microscopy-at-microscopy.com} 10, 24 -- Subject: re: water drop } microscope - p.s. 10, 24 -- Date: Tue, 4 Oct 2005 10:36:49 -0500 10, } 24 -- Message-ID: {000001c5c8f9$6ff70a00$f19bd280-at-paklabpgrover} 10, } 24 -- MIME-Version: 1.0 10, 24 -- Content-Type: text/plain; 10, 24 -- } charset="us-ascii" 10, 24 -- X-Priority: 3 (Normal) 10, 24 -- } X-MSMail-Priority: Normal 10, 24 -- X-Mailer: Microsoft Outlook, Build } 10.0.4510 10, 24 -- Importance: Normal 10, 24 -- X-MimeOLE: Produced } By Microsoft MimeOLE V6.00.2900.2180 10, 24 -- X-PMX-Version: } 4.7.1.128075 10, 24 -- X-PerlMx-Virus-Scanned: Yes 10, 24 -- } Content-Transfer-Encoding: 8bit 10, 24 -- X-MIME-Autoconverted: from } quoted-printable to 8bit by ns.microscopy.com id j94FanLt027960 } ==============================End of - } Headers==============================
Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 9, 20 -- From jbs-at-temple.edu Tue Oct 4 11:06:22 2005 9, 20 -- Received: from imp1.temple.edu (imp1.ocis.temple.edu [155.247.166.81]) 9, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j94G6M6e013074 9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 11:06:22 -0500 9, 20 -- Received: from JBS (jbs.bio.temple.edu [155.247.98.40]) 9, 20 -- by imp1.temple.edu (8.12.3/8.11.3/SuSE Linux 8.11.1-0.5) with ESMTP id j94G6K5w017571; 9, 20 -- Tue, 4 Oct 2005 12:06:20 -0400 9, 20 -- From: "Joel Sheffield" {jbs-at-temple.edu} 9, 20 -- To: pgrover-at-bilbo.bio.purdue.edu, microscopy-at-microscopy.com 9, 20 -- Date: Tue, 04 Oct 2005 12:04:18 -0400 9, 20 -- MIME-Version: 1.0 9, 20 -- Subject: Re: [Microscopy] re: water drop microscope - p.s. 9, 20 -- Reply-to: jbs-at-temple.edu 9, 20 -- Message-ID: {43426FC2.16127.4C3B2C66-at-localhost} 9, 20 -- Priority: normal 9, 20 -- In-reply-to: {200510041536.j94FaupG028179-at-ns.microscopy.com} 9, 20 -- X-mailer: Pegasus Mail for Windows (4.21c) 9, 20 -- Content-type: text/plain; charset=US-ASCII 9, 20 -- Content-transfer-encoding: 7BIT 9, 20 -- Content-description: Mail message body ==============================End of - Headers==============================
I think your being a little overly cautious - which should not hurt, but may take longer in CO2 flushing to remove (plus sends a lot of EtOH out the CPD exhaust to evaporate which generally appears be against most chemical waste protocols, which I will not argue either way)
In my experience the rule is: Always keep the samples wetted, prevent "air drying". We usually process CPD samples inside of little containers (metal baskets or scintered teflon, i.e. marshmallow baskets, or coverslip racks). We load the samples into the containers under 100% EtOH, and then transfer the containers to the CPD chamber. Alot of EtOH gets transfered with the samples into the CPD chamber. (To test this place a basket into EtOH, carefully remove it from the EtOH with forceps, and then give it a hard shake over a counter top. You will see alot of EtOH coming from the basket). This EtOH carries over long enough for us to replace the CPD chamber lid, tighten down the retaining bolts, and crack open the CO2 fill valve. As soon as some CO2 hits the chamber: (1) the expanding gas cools the chamber atmosphere, (2) rapidly increases the chamber pressure, and (3) starts wetting the samples with CO2. The first two conditions slow and stop the vaporization of the EtOH, and the third wets the sample with a new solution.
As I tell my students the only "rush" period in CPD preparation is from the moment the sample baskets are removed from the 100% solvent and the CO2 fill valve is cracked open (cracked open slowly to prevent throwning the sample basket(s) around inside the CPD chamber as the CO2 rushes into fill the chamber.
And as a followup to this: Never allow the CO2 level to fall below the sample height during the CO2 flushes. Until you are transitioning to the critical point (in which case you are also dealing with a saturated liquid CO2 environment anyway).
On 3 Oct 2005, at 16:04, wpchan-at-u.washington.edu wrote:
} } Hi } } I have a question about one of the steps in CPD using ethanol and CO2. } We have both a Denton DCP-1 and a Bomer 900EX. I was taught that I should } never let the sample exposed to air after passing the higher alcohol } concentrations e.g., 75% and above. When transferring the dehydrated } sample from the ethanol into the CPD chamber, I will fill the chamber with } enough ethanol to submerge the prep and then transfer the sample } basket/container quickly into the CPD chamber. However, I have come } across quite a few protocols that either do not specify this or simply } fill the chamber with CO2 "snow". } } So my question is, should I fill the chamber with ethanol? Am I being } overly cautious or have I missed anything critical (no puns intended!)? } Can this be sample specific e.g., smaller or delicate samples may be } more prone to surface tension disruption? Any advice and comments are } much appreciated. } } -- } Pang (Wai Pang Chan, wpchan-at-u.washington.edu) }
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"WE ARE MICROSOFT. RESISTANCE IS FUTILE. YOU WILL BE ASSIMILATED."
==============================Original Headers============================== 10, 24 -- From edelmare-at-muohio.edu Tue Oct 4 11:09:01 2005 10, 24 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 10, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j94G91N5015894 10, 24 -- for {microscopy-at-Microscopy.com} ; Tue, 4 Oct 2005 11:09:01 -0500 10, 24 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 10, 24 -- by mulnx11.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j94G8scT030472; 10, 24 -- Tue, 4 Oct 2005 12:08:54 -0400 10, 24 -- Received: from emf03 ([134.53.14.97]) 10, 24 -- by mulnx23.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j94G8rh4019740; 10, 24 -- Tue, 4 Oct 2005 12:08:53 -0400 10, 24 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 10, 24 -- To: wpchan-at-u.washington.edu, microscopy-at-Microscopy.com 10, 24 -- Date: Tue, 4 Oct 2005 12:08:59 -0400 10, 24 -- MIME-Version: 1.0 10, 24 -- Content-type: text/plain; charset=US-ASCII 10, 24 -- Content-transfer-encoding: 7BIT 10, 24 -- Subject: Re: [Microscopy] CPD 10, 24 -- Message-ID: {434270DB.18027.5C724DA-at-localhost} 10, 24 -- Priority: normal 10, 24 -- In-reply-to: {200510032104.j93L4jPf004168-at-ns.microscopy.com} 10, 24 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 10, 24 -- X-Real-ConnectIP: 134.53.14.97 10, 24 -- X-Scanned-By: MIMEDefang 2.45 10, 24 -- X-Scanned-By: MIMEDefang 2.52 on 134.53.6.10 ==============================End of - Headers==============================
Actually Leeuwenhoek used ground and polished glass lenses, probably made with a tube lap. He was certainly familiar with "blown" or fused lenses which, however, usually do not have the accuracy of curvature required for the best optical performance.
Details on how to grind and polish short focus bead lenses and various other ways to make homemade microscope objectives with high resolution are given here:
Leeuwenhoek's highest resolution discoveries must certainly have been made with the front lens in wet contact with the specimen, since the laws of optical physics do not permit the sub-micron resolution needed to observe bacteria and the correct tail length of spermatozoa; a one micron resolution is about the best that can be done with a dry lens.
These conclusions are more fully discussed in my article in "The Microscope"; "Leeuwenhoek, the Short-focus Lens, and the History of Optics; an Experimental Inquiry" in the first quarter of 1993.
The known facts seem to indicate that Leeuwenhoek must have both invented the immersion objective and used a double lens, although he used and revealed various single lens microscopes too. This explains a number of historical facts, including Leeuwenhoek's claim that he did not reveal his best microscopes to others.
-- Roger, Austin
On Oct 4, 2005, at 10:39 AM, pgrover-at-bilbo.bio.purdue.edu wrote:
} } For some reason, if you just click on the url, the last part of it } doesn't } copy, and you won't get all the way to the web page. You may have } to cut 'n } paste the last part, or type it in, but it's worth the trouble! } } } Joel, } } Check out } } http://www.microscopy-uk.net/mag/indexmag.html?http:// } www.microscopy-uk.net/ } mag/art98/watermic.html } } I haven't made a water drop microscope, but I have been making } Leeuwenhoek } glass ball microscope replicas. I haven't yet honed my lensmaking } skills to } resolve bacilli, spirilla, cocci, like he did, but diatoms look } awesome } through it! I just twirl a glass thread in a flame to make the } spheres, but } I don't think I have the patience to do the polishing that he did. } I may } cheat and buy one of the Edmund Optics glass ball lenses (~$20).
==============================Original Headers============================== 12, 19 -- From rcbaker-at-eden.infohwy.com Tue Oct 4 11:26:45 2005 12, 19 -- Received: from mx1.lsn.net (mx1.lsn.net [66.90.130.73]) 12, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j94GQjLN030330 12, 19 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 11:26:45 -0500 12, 19 -- Received: from [192.168.1.103] (66-90-146-134.dyn.grandenetworks.net [66.90.146.134]) 12, 19 -- by mx1.lsn.net (8.13.0.Beta3/8.13.0.Beta3) with ESMTP id j94GQhJQ014778 12, 19 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 11:26:48 -0500 12, 19 -- Mime-Version: 1.0 (Apple Message framework v734) 12, 19 -- In-Reply-To: {200510041539.j94FdsQ8001062-at-ns.microscopy.com} 12, 19 -- References: {200510041539.j94FdsQ8001062-at-ns.microscopy.com} 12, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 12, 19 -- Message-Id: {4D6ACA47-715C-4F79-8492-77F974812945-at-eden.infohwy.com} 12, 19 -- Content-Transfer-Encoding: 7bit 12, 19 -- From: Roger Baker {rcbaker-at-eden.infohwy.com} 12, 19 -- Subject: Re: [Microscopy] re: water drop microscope - p.s. 12, 19 -- Date: Tue, 4 Oct 2005 11:26:31 -0500 12, 19 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 12, 19 -- X-Mailer: Apple Mail (2.734) 12, 19 -- X-Antivirus: Scanned by Vexira Antivirus 1.1.6 ==============================End of - Headers==============================
Further on the discussion on Glyptal, this was once all part of General Electric, and in 1985, the business unit was spun out of GE and now operates as
GLYPTAL, INC. 305 Eastern Ave. Chelsea, Ma 02150 (617) 884-6918 1-800-GLP-1201 FAX (617) 884-8376 E-MAIL billhoag-at-comcast.net
The original products made by GE were formulated to be insulating paints for electrical applications. I can remember back in my days at the DuPont Experimental Station, and I am talking about the late 1960's, one of the technicians (Bob Schatz, who was a "teacher" for more than just a few of us on this listserver) considered his little bottle of Glyptal liquid to be one of his most valuable possessions. He used it on the bell jar gaskets when they developed cracks, among other things. However I don't recall his ever referring to it as anything other than "Glyptal", even though there is an entire list of products sold under the trade name Glyptal.
However, with the passing of time, there have emerged better vacuum leak sealant materials, some having been previously mentioned, such as VacSeal. Another one but not previously mentioned is CelvaSeal. We ourselves prefer VacSeal because of its lower viscosity. Both end up being cured silicones and when one wants to remove them, they won't come off with some magic solvent. But I would assume that there is some temperature where they would come off but probably before reaching traditional "bake out" temperatures.
Disclaimer: SPI Supplies offers both VacSeal and CelvaSeal vacuum leak sealants on the SPI Supplies website (see below).
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.2spi.com ######################## ============================================
==============================Original Headers============================== 12, 26 -- From cgarber-at-2spi.com Tue Oct 4 11:43:04 2005 12, 26 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 12, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j94Gh49I006639 12, 26 -- for {microscopy-at-msa.microscopy.com} ; Tue, 4 Oct 2005 11:43:04 -0500 12, 26 -- Received: from Desktop (pcp02988842pcs.malvrn01.pa.comcast.net [68.85.250.247]) 12, 26 -- (authenticated bits=0) 12, 26 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id j94Gh1bS013430 12, 26 -- for {microscopy-at-msa.microscopy.com} ; Tue, 4 Oct 2005 12:43:03 -0400 12, 26 -- X-IDV-FirstRcvd: pcp02988842pcs.malvrn01.pa.comcast.net [68.85.250.247] 12, 26 -- X-IDV-HELO: Desktop 12, 26 -- X-IDV-Authenticated-User: cgarber 12, 26 -- Message-ID: {016c01c5c902$b77605d0$6301a8c0-at-Desktop} 12, 26 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 12, 26 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 12, 26 -- Subject: Glyptal products 12, 26 -- Date: Tue, 4 Oct 2005 12:43:14 -0400 12, 26 -- MIME-Version: 1.0 12, 26 -- Content-Type: text/plain; 12, 26 -- format=flowed; 12, 26 -- charset="iso-8859-1"; 12, 26 -- reply-type=original 12, 26 -- Content-Transfer-Encoding: 7bit 12, 26 -- X-Priority: 3 12, 26 -- X-MSMail-Priority: Normal 12, 26 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 12, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
Assistant Research Scientist Position in Material Characterization
The Center for Advanced Ultrastructural Research (CAUR) at University of Georgia announces an opening for a material characterization specialist in the field of electron microscopy and X-ray microanalysis. The CAUR supports seven major research instruments --- two SEMs, two TEMs, a multiphoton/confocal microscope (polarization/DIC capable), an X-ray microtomography unit, and a digital inverted fluorescence microscope. The center has a large and growing user community in the physical, material, and geological sciences. This person will be hired at the level of Research Assistant Scientist, which is a long-term promotion-track position. Candidates should have a publication record and commensurate skills in materials science, electron diffraction, and sample preparation of both synthetic and natural materials. A Ph.D. or equivalent experience in the physical sciences required. The individual will be responsible for training and supervising users for TEM, SEM, X-ray microtomography, collaboration on research proposals, and will have an opportunity for conducting and publishing research in their own area of expertise. Applicants should submit a curriculum vitae, statement of research interests, and contact information for three references to Prof. Charles Keith, Director, Center for Advanced Ultrastructural Research, 151 Barrow Hall, University of Georgia, Athens, GA 30602. chkeith-at-cb.uga.edu. Application review will start on November 1, 2005. Applications received by that date are assured consideration. UGA is an Equal Opportunity/Affirmative Action Institution.
John P. Shields Center for Ultrastructural Research 151 Barrow Hall University of Georgia Athens, GA 30602
706-542-4080
==============================Original Headers============================== 4, 21 -- From jpshield-at-uga.edu Tue Oct 4 13:30:40 2005 4, 21 -- Received: from puntd5.cc.uga.edu (puntd5.cc.uga.edu [128.192.1.108]) 4, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j94IUdOu016957 4, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Oct 2005 13:30:40 -0500 4, 21 -- Received: from punts4.cc.uga.edu (punts4.cc.uga.edu [128.192.1.115]) 4, 21 -- by puntd5.cc.uga.edu (MOS 3.5.9-GR) 4, 21 -- with ESMTP id CBY42616; 4, 21 -- Tue, 4 Oct 2005 14:30:35 -0400 (EDT) 4, 21 -- Received: from 128.192.63.198 4, 21 -- by punts4.cc.uga.edu (MOS 3.5.9-GR) 4, 21 -- with HTTPS/1.1; 4, 21 -- Tue, 4 Oct 2005 14:30:35 -0400 4, 21 -- Date: Tue, 4 Oct 2005 14:30:35 -0400 4, 21 -- From: John Shields {jpshield-at-uga.edu} 4, 21 -- Subject: EM job - material/physical 4, 21 -- To: msa listserve {Microscopy-at-MSA.Microscopy.Com} 4, 21 -- X-Mailer: Mirapoint Webmail Direct 3.5.9-GR 4, 21 -- MIME-Version: 1.0 4, 21 -- Message-Id: {e1e26fde.4616fc60.8293600-at-punts4.cc.uga.edu} 4, 21 -- Content-Type: text/plain; charset=us-ascii 4, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello, We are looking for working´s TEM and SEM with manuals to receive it in donation. Our non profit organization will begin teaching and I+ D proyects with this equipment We will pay shipping and handling costs
thanks in advance
Fernando Balducci President -- Fundatel Fundación de Telemedicina Gualeguaychú 878 Dpto 3 Paraná (ER) - Argentina TE: +54-(0)343-4221716 www.fundatel.org.ar
==============================Original Headers============================== 4, 24 -- From fundatel-at-gmail.com Tue Oct 4 14:34:42 2005 4, 24 -- Received: from xproxy.gmail.com (xproxy.gmail.com [66.249.82.197]) 4, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j94JYgme026823 4, 24 -- for {Microscopy-at-Microscopy.Com} ; Tue, 4 Oct 2005 14:34:42 -0500 4, 24 -- Received: by xproxy.gmail.com with SMTP id s17so127031wxc 4, 24 -- for {Microscopy-at-Microscopy.Com} ; Tue, 04 Oct 2005 12:34:41 -0700 (PDT) 4, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 4, 24 -- s=beta; d=gmail.com; 4, 24 -- h=received:message-id:date:from:reply-to:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 24 -- b=RJQ31/uaKnzLcjfJPSyfFsjGzzC5Azya9okVrvJQ26nPwzZXgAlV+h2wCVYeUBKjm06yXToNWSDaIo7jyVvmuaVF4nCFoLLzB4lcEPQB//K7/db/t2e/fTby6+mMuixpC4tkGEjl1tYBI9LGsx5CswwG2zEwoXq+fjp+MQvZ0D0= 4, 24 -- Received: by 10.70.105.13 with SMTP id d13mr959145wxc; 4, 24 -- Tue, 04 Oct 2005 12:34:41 -0700 (PDT) 4, 24 -- Received: by 10.70.59.15 with HTTP; Tue, 4 Oct 2005 12:34:41 -0700 (PDT) 4, 24 -- Message-ID: {715613900510041234x296a4524k-at-mail.gmail.com} 4, 24 -- Date: Tue, 4 Oct 2005 16:34:41 -0300 4, 24 -- From: Fundatel {fundatel-at-gmail.com} 4, 24 -- Reply-To: Fundatel {fundatel-at-gmail.com} 4, 24 -- To: Microscopy-at-Microscopy.Com 4, 24 -- Subject: Looking for SEM and TEM 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; charset=ISO-8859-1 4, 24 -- Content-Disposition: inline 4, 24 -- Content-Transfer-Encoding: 8bit 4, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j94JYgme026823 ==============================End of - Headers==============================
Dear Colleagues, the Foundation of the “Freiburg Center for Systems Biology” (ZBSA) at the Albert-Ludwigs-University of Freiburg will take place on November 3, 2005.
Linked to this event is an
*International Imaging Symposium November 4-5, 2005*
*"From static spots to dynamic proteome visualization and beyond"*
organized by the Life Imaging Center Freiburg and sponsored by DFG, BMBF and SFBs 388, 505, 592.* * List of speakers:
F. Brandizzi, Saskatoon (CDN) W. Denk, Heidelberg (D) M. Dickinson, Houston (USA) TWJ. Gadella, Amsterdam (NL) G. Galizia, Konstanz (D) H. Gerdes, Heidelberg (D) M. Heisler, Pasadena (USA) T. Hirano, Tskuba-Higashi (J) T. Kerpolla, Ann Arbor (USA) P. Lipp, Homburg (D) J. Lippincott-Schwartz, Bethesda (USA) T. Meyer, Stanford (USA) R. Murphy, Pittsburgh (USA) L. Nedbal, Nove Hrady (CZ) M. Oheim, Paris (F) H. Okamoto, Wako City (J) R. Pepperkok, Heidelberg (D) W. Schubert, Magdeburg (D) E. Stelzer, Heidelberg (D) D. Toomre, New Haven (USA) R. Uhl, Martinsried (D) F. Wouters, Göttingen (D) W. Zipfel, Ithaca (USA)
Further information at: http://www.zbsa.uni-freiburg.de/seminar/event.php
If you are interested in the Systems Biology Symposium please look here: Foundation of the Center for Systems Biology (ZBSA) November 3, 2005 http://www.zbsa.uni-freiburg.de/seminar/events.php
Detailed meeting programs and registration and accomodation information are available on the web.
We are looking forward to see you in Freiburg.
Roland Nitschke and Klaus Palme
___________________________ Nitschke, Roland Dr. Life Imaging Center Institute of Biology Albert-Ludwigs-University Freiburg Hauptstr.1 D-79104 Freiburg Germany ___________________________ E-mail: Roland.Nitschke-at-biologie.uni-freiburg.de phone: 49-761-2032934 or 2902 fax: 49-761-2032941 http://www.sfb592.uni-freiburg.de/z2/index.html
==============================Original Headers============================== 15, 25 -- From Roland.Nitschke-at-biologie.uni-freiburg.de Tue Oct 4 14:50:11 2005 15, 25 -- Received: from mailgateway1.uni-freiburg.de (mailgateway1.uni-freiburg.de [132.230.2.211]) 15, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j94JoADD003354 15, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Oct 2005 14:50:11 -0500 15, 25 -- Delivery-date: Tue, 04 Oct 2005 21:50:10 +0200 15, 25 -- Received: from biologie.uni-freiburg.de ([132.230.2.43] helo=uni-freiburg.de) port 51768 15, 25 -- by mailgateway1.uni-freiburg.de with esmtp 15, 25 -- (Exim 4.50 #1 built 18-Feb-2005 14:34:25 running on Gentoo) 15, 25 -- id 1EMsnh-0005Eg-F1 15, 25 -- for Microscopy-at-microscopy.com; Tue, 04 Oct 2005 21:50:09 +0200 15, 25 -- Received: from [132.230.96.118] (account roland.nitschke-at-biologie.uni-freiburg.de HELO [132.230.96.118]) 15, 25 -- by uni-freiburg.de (CommuniGate Pro SMTP 4.3.8) 15, 25 -- with ESMTPSA id 62642895 for Microscopy-at-Microscopy.Com; Tue, 04 Oct 2005 21:49:59 +0200 15, 25 -- Message-ID: {4342DCF2.2050201-at-biologie.uni-freiburg.de} 15, 25 -- Date: Tue, 04 Oct 2005 21:50:10 +0200 15, 25 -- From: Roland Nitschke {Roland.Nitschke-at-biologie.uni-freiburg.de} 15, 25 -- Reply-To: Roland.Nitschke-at-biologie.uni-freiburg.de 15, 25 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 15, 25 -- X-Accept-Language: en-us, en 15, 25 -- MIME-Version: 1.0 15, 25 -- To: Microscopy-at-microscopy.com 15, 25 -- Subject: Announcment for Imaging Symposium "From static spots to dynamic proteome 15, 25 -- visualization and beyond", Freiburg, Germany 04. - 05.11.2005 15, 25 -- Content-Type: text/plain; charset=windows-1252 15, 25 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (classiccarguy89-at-aol.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, September 29, 2005 at 22:08:06 ---------------------------------------------------------------------------
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (patricia.correia-at-kcl.ac.uk) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, October 5, 2005 at 03:35:27 ---------------------------------------------------------------------------
Question: I am interested in measuring the size of salivary glands acini to make some comparisons as to either they are atrophic or not. I have been using tissue samples formaline fixed in a optic microscope with the programme analySIS from "Soft Imaging System" (www.soft-imaging.net), which allows us to contour the area of each acini and then gives its reading. Do you know of any better method of measuring it? The current method I'm using implies a great deal of subjectivity: depending on the tissue section, the criteria to select the acini and so forth.
Thank you for your attention. Best regards, Patricia Correia
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (beez1-at-bigpond.com) from on Wednesday, October 5, 2005 at 05:25:37 ---------------------------------------------------------------------------
Email: beez1-at-bigpond.com Name: Rohan Wighton
Organization: Charles Sturt University
Title-Subject: [Filtered] MListserver:
Question: Hi everyone
I have just become the proud owner of a Nikon S-Ke coaxial focusing control type microscope. It has a nikon phase contrast turret. I have several problems, which I list in order of relative importance:
1. I have no transformer for the lamp, and having looked briefly on the net for somewhere to find and buy a replacement, I am beginning to think I might have trouble. Can anyone suggest a contact for this?
2. The knob used to adjust the distance between the eyepieces seems stuck (although the scope is in excellent condition this does not move at all) Will lubrication do the job? If so, what do I use? If not, do I send the scope to an expert?
3. As you may have guessed by now I am a beginner, my most extensive binocular work having been dissecting flowers for a semester (loved it!) Any advice for me and/or some history on my particular model scope would be much appreciated.
Get yourself a copy of "Unbiased Sterology" by C.V. Howard and M.G. Reed. In order to make an accurate assessment of your 'target' you must get rid of subjectivity in your sampling.
Geoff
patricia.correia-at-kcl.ac.uk wrote:
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-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 9, 33 -- From mcauliff-at-umdnj.edu Wed Oct 5 09:24:54 2005 9, 33 -- Received: from mail01.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126]) 9, 33 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j95EOsjn018467 9, 33 -- for {microscopy-at-msa.microscopy.com} ; Wed, 5 Oct 2005 09:24:54 -0500 9, 33 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1]) 9, 33 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 0D6C523005F 9, 33 -- for {microscopy-at-msa.microscopy.com} ; Wed, 5 Oct 2005 10:24:54 -0400 (EDT) 9, 33 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 9, 33 -- by mail01.umdnj.edu (Proprietary) with ESMTP id D0C54EC0B9 9, 33 -- for {microscopy-at-msa.microscopy.com} ; Wed, 5 Oct 2005 10:24:52 -0400 (EDT) 9, 33 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 9, 33 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 9, 33 -- id {0INW00F015B7T9-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 9, 33 -- for microscopy-at-msa.microscopy.com; Wed, 05 Oct 2005 10:24:52 -0400 (EDT) 9, 33 -- Received: from [127.0.0.1] ([10.138.2.240]) 9, 33 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 9, 33 -- 2004)) with ESMTP id {0INW00CNJ52XPH-at-Polaris.umdnj.edu} ; Wed, 9, 33 -- 05 Oct 2005 10:18:33 -0400 (EDT) 9, 33 -- Date: Wed, 05 Oct 2005 10:18:44 -0400 9, 33 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 9, 33 -- Subject: Re: [Microscopy] AskAMicroscopist: measuring the size of salivary 9, 33 -- glands 9, 33 -- In-reply-to: {200510051320.j95DKRWQ030632-at-ns.microscopy.com} 9, 33 -- To: patricia.correia-at-kcl.ac.uk, 9, 33 -- MicroscopyListserver {microscopy-at-msa.microscopy.com} 9, 33 -- Message-id: {4343E0C4.9030103-at-umdnj.edu} 9, 33 -- MIME-version: 1.0 9, 33 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 9, 33 -- Content-transfer-encoding: 7BIT 9, 33 -- X-Accept-Language: en-us, en 9, 33 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 9, 33 -- Gecko/20040804 Netscape/7.2 (ax) 9, 33 -- References: {200510051320.j95DKRWQ030632-at-ns.microscopy.com} ==============================End of - Headers==============================
Dear All, We have just taken delivery of a chromium sputter coating unit and I am attempting to do a risk assessment and having read some of the MDS on Chromium am now very apprehensive about the toxicity of the fumes and flakes produced by the target. As I am not a chemist, I am unsure what I am dealing with. If anyone out there has any advice they would be willing to share, including things like should it be used in a fume hood and how to dispose of the 'flakes' of chromium, it would be greatly appreciated. Thanks in advance, Christine.
A.C.Richardson Experimental Officer School of Biological and Biomedical Science Centre for Molecular Imaging University of Durham Science site South Rd Durham England E-mail: a.c.richardson-at-durham.ac.uk
==============================Original Headers============================== 3, 24 -- From a.c.richardson-at-durham.ac.uk Wed Oct 5 10:07:14 2005 3, 24 -- Received: from hermes.dur.ac.uk (hermes.dur.ac.uk [129.234.4.9]) 3, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j95F7DVv027521 3, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Oct 2005 10:07:14 -0500 3, 24 -- Received: from smtphost2.dur.ac.uk (smtphost2.dur.ac.uk [129.234.4.209]) 3, 24 -- by hermes.dur.ac.uk (8.11.7-20030923/8.11.7) with ESMTP id j95F6hY07387 3, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Oct 2005 16:06:43 +0100 (BST) 3, 24 -- Received: from [129.234.132.186] (biol-186.dur.ac.uk [129.234.132.186]) 3, 24 -- by smtphost2.dur.ac.uk (8.12.10+Sun/8.11.7) with ESMTP id j95F6ZNg003330 3, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Oct 2005 16:06:35 +0100 (BST) 3, 24 -- Message-ID: {4343EBF4.30001-at-dur.ac.uk} 3, 24 -- Disposition-Notification-To: Christine Richardson {a.c.richardson-at-durham.ac.uk} 3, 24 -- Date: Wed, 05 Oct 2005 16:06:28 +0100 3, 24 -- From: Christine Richardson {a.c.richardson-at-durham.ac.uk} 3, 24 -- Organization: University of Durham Dept of Biological and Biomedical Science, 3, 24 -- Centre for Molecular Imaging South Road Durham DH1 3LE. 3, 24 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 3, 24 -- X-Accept-Language: en-us, en 3, 24 -- MIME-Version: 1.0 3, 24 -- To: Microscopy-at-microscopy.com 3, 24 -- Subject: TEM: Chromium sputter coating 3, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 24 -- Content-Transfer-Encoding: 7bit 3, 24 -- X-DurhamAcUk-MailScanner: Found to be clean, Found to be clean ==============================End of - Headers==============================
My best advice is to use it quickly. I do not know about toxicity but I do know that Cr oxidizes rapidly. So, be alert to this. I use Pt, Ir and Au/Pd instead of Cr.
gary g.
At 08:10 AM 10/5/2005, you wrote:
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==============================Original Headers============================== 9, 24 -- From gary-at-gaugler.com Wed Oct 5 10:35:58 2005 9, 24 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j95FZwiC004000 9, 24 -- for {microscopy-at-microscopy.com} ; Wed, 5 Oct 2005 10:35:58 -0500 9, 24 -- Received: (qmail 1328 invoked from network); 5 Oct 2005 08:35:37 -0700 9, 24 -- Received: by simscan 1.1.0 ppid: 1304, pid: 1305, t: 5.9388s 9, 24 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1105 spam: 3.0.3 9, 24 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 9, 24 -- by qsmtp3 with SMTP; 5 Oct 2005 08:35:31 -0700 9, 24 -- Message-Id: {6.2.3.4.2.20051005083342.02b19ef0-at-mail.calweb.com} 9, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 9, 24 -- Date: Wed, 05 Oct 2005 08:35:51 -0700 9, 24 -- To: a.c.richardson-at-durham.ac.uk 9, 24 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 24 -- Subject: Re: [Microscopy] TEM: Chromium sputter coating 9, 24 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 24 -- In-Reply-To: {200510051510.j95FAHl2030830-at-ns.microscopy.com} 9, 24 -- References: {200510051510.j95FAHl2030830-at-ns.microscopy.com} 9, 24 -- Mime-Version: 1.0 9, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 9, 24 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp3.surewest.net 9, 24 -- X-Spam-Level: 9, 24 -- X-Spam-Status: No, score=0.0 required=5.0 tests=none autolearn=failed 9, 24 -- version=3.0.3 ==============================End of - Headers==============================
Spirillum serpens is a spiral-shaped bacteria from the genus Spirillum, all of which which live in water (hence the aqua bit) except for one odd species that causes a type of rat-bite fever in humans (Spirillum minus). The term spirillum is used generally for any corkscrew-like shaped species of bacteria.
These Spirilla bacteria stain Gram-negative. The Gram stain is a special stain used with a lot of bacteria mounted on a glass slide. Bacteria are often classified as being positive (violet stained) or negative (stained pink), when viewed under a standard light microscope. This 'Gram stain' result is due to differences in the cell wall of the bacteria and this can be important medically e.g. it can affect how effective antibiotics are at killing them.
Spirillum bacteria move by means of tufts of flagella (wavey hairlike structures) at each end of the cell. Spirillum serpens is sometimes looked at in schools for bacteria morphology (shape) studies, presumably because it is relatively harmless and conviniently lives in water (e.g. http://heathscientific.net/item.asp?id=115 ). The bacteria Spirillum serpens should look a bit similar to the nasty Spirillum minus, see : http://www.ulb.ac.be/sciences/biodic/images/bacterie/spirillumminus.jpg where it is stained Gram negative (pink).
Further info: Gram stain pictures of bacteria can be seen at http://www.ncl.ac.uk/dental/oralbiol/oralenv/tutorials/gramstain.htm It's a bit technical but the pictures show how to stain a slide that a lot of bacteria dried onto the surface.
Regards, Keith
PS. I had a look at my copy of the Bioaerosols Handbook, 1995 CRC Press Inc (I wrote chapter 11 - Modern microscopicial methods) but these spirella bacteria aren't covered, probably as they live in water (aqua) not the air (aero) which I was interested in. I've never looked at them under a microscope, so I can't add much more than the above, but I expect that has already told as much as you wanted to know about the Spirilla bacteria.
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
----- Original Message ----- X-from: {classiccarguy89-at-aol.com} To: {keith.morris-at-ucl.ac.uk} Sent: Wednesday, October 05, 2005 2:29 PM
We typically dehydrate our samples (e.g. mouse embryos) in increasing concentrations of EtOH and then change it to amyl acetate (AAC). This has three advantages -
a) AAC is less volatile than EtOH - we do not have problems with air drying samples during transfer b) supposedly, AAC mixes better with liquid CO2 c) AAC has a specific aroma - it's absence is a good indicator the sample is ready for CPD.
Michal
edelmare-at-muohio.edu wrote:
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==============================Original Headers============================== 7, 19 -- From M_Jarnik-at-fccc.edu Wed Oct 5 11:08:51 2005 7, 19 -- Received: from azah.fccc.edu (azah.fccc.edu [131.249.4.237]) 7, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j95G8oGS021477 7, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Oct 2005 11:08:51 -0500 7, 19 -- Received: from fccc.edu (emf4.fccc.edu [131.249.9.170]) 7, 19 -- by azah.fccc.edu (8.12.11/8.12.11) with ESMTP id j95G8oYW017664 7, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Oct 2005 12:08:50 -0400 (EDT) 7, 19 -- Message-ID: {4343FA90.4080405-at-fccc.edu} 7, 19 -- Date: Wed, 05 Oct 2005 12:08:48 -0400 7, 19 -- From: Michal Jarnik {M_Jarnik-at-fccc.edu} 7, 19 -- Reply-To: M_Jarnik-at-fccc.edu 7, 19 -- Organization: Fox Chase Cancer Center 7, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 7, 19 -- X-Accept-Language: en,cs 7, 19 -- MIME-Version: 1.0 7, 19 -- To: Microscopy-at-microscopy.com 7, 19 -- Subject: Re: CPD 7, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 7, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have a colleague who who like to correspond with anyone who has successfully used a MOHR PRO 8 "ME-42" film processor for Kodak 4489 TEM film. He'd like to know what settings (temp, transport speed, develop time etc) are best. Thanks in advance if you can help.
Best Regards, Kirk
Kirk J. Czymmek, Ph.D. Associate Professor Department of Biological Sciences University of Delaware Newark, DE 19713 kirk-at-udel.edu
==============================Original Headers============================== 7, 20 -- From kirk-at-UDel.Edu Wed Oct 5 11:34:28 2005 7, 20 -- Received: from copland.udel.edu (copland.udel.edu [128.175.13.92]) 7, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j95GYRA2030234 7, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Oct 2005 11:34:28 -0500 7, 20 -- Received: from [128.175.253.124] (host-253-124.nss.udel.edu [128.175.253.124]) 7, 20 -- by copland.udel.edu (8.12.11/8.12.11) with ESMTP id j95GYOeL019277 7, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Oct 2005 12:34:24 -0400 (EDT) 7, 20 -- Message-ID: {4344054A.2060001-at-udel.edu} 7, 20 -- Date: Wed, 05 Oct 2005 12:54:34 -0400 7, 20 -- From: kirk czymmek {kirk-at-UDel.Edu} 7, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.7.8) Gecko/20050511 7, 20 -- X-Accept-Language: en-us, en 7, 20 -- MIME-Version: 1.0 7, 20 -- To: Microscopy-at-microscopy.com 7, 20 -- Subject: Kodak 4489 TEM film devlopment with Mohr Pro 8 7, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit 7, 20 -- X-CanItPRO-Stream: default 7, 20 -- X-Spam-Score: 0 () 7, 20 -- X-Scanned-By: CanIt (www . canit . ca) ==============================End of - Headers==============================
I am working on designing a new electron detector for our SEM. Does anyone have an extra PMT with/without a head amplifier? I'm looking for one that matches the P47 emission spectrum.
If anyone has one just sitting around and gathering dust, I could use it for experimenting. I would gladly pay for shipping fees.
Thanks, Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC W6-017A ECERF Bldg, 9107-116 Street Edmonton, AB. T6G 2V4
There is little to worry about. The nasty Cr is Cr in the hexavalent state (+6). The Cr of your taget is metallic with a thin oxide on it. The natural oxide of Cr is Cr2O3 which means that the Cr is in the +3 state and is bound up with the oxide.
The process of sputtering is physical bombardment of the target with Ar ions that removes the atoms from the near surface of the target. The process is line of sight deposition onto your substrate and metallic Cr is being deposited (valence state is 0). That is why you need a rotating and tilting sample to get a uniform and continuos coating on your sample for high resolution imaging in the SEM. I assume that the flakes that you are talking about are flakes in the deposition chamber. If you are getting flakes of material on your chamber walls, you must be putting down very thick coatings or very many coatings. Our IBS/e sputter coater can put down a uniform, continuous coating that is less than 10 Angstroms. Your coater should be capable of doing the same. If you deposit these types of films, it should take a long time before you start to get flakes in your system which is thick coatings that peel from the walls because of high stresses in the films. Regardless, in the vacuum system, what is deposited is the metal Cr and it oxides to Cr2O3 when exposed to air when you open the chamber. When you clean your chamber and there is dust or flakes, wear a mask and discard the cleaned material as you would a heavy metal. There will be no Cr fumes anywhere. Cr will not be present in the pump exhaust.
As Gary Gaughler said in his reply, Cr does oxidize fairly rapidly, so samples coated with it must be run soon after. The natural protective oxide that forms on Cr is about the thickness of the coating that you want on your sample. When you consider how thin the thickness of the coating is and how long they can be exposed to atmosphere the actual oxidation rate is not all that high. South Bay Technology has just introduced the SampleSaver(TM) storage container to help prevent this and maintain sample in an inert atmosphere. It is generally accepted that Cr coatings give the best results, but Ir and W coatings approach the quality of Cr and are less susceptible to complete film oxidation. Pt, Au, and Au-Pd coatings do not give as fine a grain size as the other coatings.
Disclaimer: South Bay Technology, Inc. manufactures and sells the IBS/e ion sputter coater and etching system and the SampleSave(TM) storage container.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
-------- Original Message -------- } From: a.c.richardson-at-durham.ac.uk } Sent: Wednesday, October 05, 2005 11:12 AM } To: Walck-at-SouthBayTech.com } Subject: [Microscopy] TEM: Chromium sputter coating } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Dear All, } We have just taken delivery of a chromium sputter coating unit and I am } attempting to do a risk assessment and having read some of the MDS on } Chromium am now very apprehensive about the toxicity of the fumes and } flakes produced by the target. As I am not a chemist, I am unsure what I } am dealing with. If anyone out there has any advice they would be } willing to share, including things like should it be used in a fume hood } and how to dispose of the 'flakes' of chromium, it would be greatly } appreciated. Thanks in advance, } Christine. } } A.C.Richardson } Experimental Officer } School of Biological and Biomedical Science } Centre for Molecular Imaging } University of Durham } Science site } South Rd } Durham } England } E-mail: a.c.richardson-at-durham.ac.uk } } ==============================Original Headers============================== } 3, 24 -- From a.c.richardson-at-durham.ac.uk Wed Oct 5 10:07:14 2005 } 3, 24 -- Received: from hermes.dur.ac.uk (hermes.dur.ac.uk [129.234.4.9]) } 3, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j95F7DVv027521 } 3, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Oct 2005 10:07:14 -0500 } 3, 24 -- Received: from smtphost2.dur.ac.uk (smtphost2.dur.ac.uk [129.234.4.209]) } 3, 24 -- by hermes.dur.ac.uk (8.11.7-20030923/8.11.7) with ESMTP id j95F6hY07387 } 3, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Oct 2005 16:06:43 +0100 (BST) } 3, 24 -- Received: from [129.234.132.186] (biol-186.dur.ac.uk [129.234.132.186]) } 3, 24 -- by smtphost2.dur.ac.uk (8.12.10+Sun/8.11.7) with ESMTP id j95F6ZNg003330 } 3, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Oct 2005 16:06:35 +0100 (BST) } 3, 24 -- Message-ID: {4343EBF4.30001-at-dur.ac.uk} } 3, 24 -- Disposition-Notification-To: Christine Richardson {a.c.richardson-at-durham.ac.uk} } 3, 24 -- Date: Wed, 05 Oct 2005 16:06:28 +0100 } 3, 24 -- From: Christine Richardson {a.c.richardson-at-durham.ac.uk} } 3, 24 -- Organization: University of Durham Dept of Biological and Biomedical Science, } 3, 24 -- Centre for Molecular Imaging South Road Durham DH1 3LE. } 3, 24 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) } 3, 24 -- X-Accept-Language: en-us, en } 3, 24 -- MIME-Version: 1.0 } 3, 24 -- To: Microscopy-at-microscopy.com } 3, 24 -- Subject: TEM: Chromium sputter coating } 3, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 3, 24 -- Content-Transfer-Encoding: 7bit } 3, 24 -- X-DurhamAcUk-MailScanner: Found to be clean, Found to be clean } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 23 -- From walck-at-southbaytech.com Wed Oct 5 11:52:11 2005 5, 23 -- Received: from smtp03.safesecureweb.com (smtp03.safesecureweb.com [65.36.154.50]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j95GqBgn015156 5, 23 -- for {microscopy-at-microscopy.com} ; Wed, 5 Oct 2005 11:52:11 -0500 5, 23 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 5, 23 -- by smtp03.safesecureweb.com (Postfix) with ESMTP id D8D68381D8; 5, 23 -- Wed, 5 Oct 2005 12:52:10 -0400 (EDT) 5, 23 -- Received: from mail15.safesecureweb.com (unknown [192.168.2.180]) 5, 23 -- by smtp03.safesecureweb.com (Postfix) with ESMTP id 00BB438093; 5, 23 -- Wed, 5 Oct 2005 12:52:06 -0400 (EDT) 5, 23 -- MIME-Version: 1.0 5, 23 -- Date: Wed, 5 Oct 2005 12:50:51 -0400 5, 23 -- Content-Type: text/plain; 5, 23 -- charset=iso-8859-1 5, 23 -- Subject: re: [Microscopy] TEM: Chromium sputter coating 5, 23 -- From: "Scott Walck" {walck-at-southbaytech.com} 5, 23 -- Reply-To: Walck-at-southbaytech.com 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- CC: {a.c.richardson-at-durham.ac.uk} 5, 23 -- Message-ID: {0beb5c189a8a4231b7d42be6af9313b5-at-southbaytech.com} 5, 23 -- X-Virus-Scanned: by amavisd-new-20030616-p10 at safesecureweb.com 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j95GqBgn015156 ==============================End of - Headers==============================
I am interested in companies/units that do TEM on a "pay-per-play" basis. I have a small number of bacterial samples on for which pretty routine workup and picture-snapping is required. Please contact me off-list. -- Robert J. Palmer Jr., Ph.D. Natl Inst Dental Craniofacial Res - Natl Insts Health Oral Infection and Immunity Branch Bldg 30, Room 310 30 Convent Drive Bethesda MD 20892 ph 301-594-0025 fax 301-402-0396
==============================Original Headers============================== 1, 18 -- From rjpalmer-at-dir.nidcr.nih.gov Wed Oct 5 11:57:19 2005 1, 18 -- Received: from wimpy.net.nih.gov (wimpy.net.nih.gov [128.231.88.106]) 1, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j95GvJ1S020221 1, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 5 Oct 2005 11:57:19 -0500 1, 18 -- Received: from wimpy.net.nih.gov (localhost [127.0.0.1]) 1, 18 -- by wimpy.net.nih.gov (8.12.11/8.11.7) with ESMTP id j95GvIHD025789 1, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 5 Oct 2005 12:57:18 -0400 (EDT) 1, 18 -- Received: from [128.231.107.215] (nidcr107-215.nidcr.nih.gov [128.231.107.215]) 1, 18 -- by wimpy.net.nih.gov (8.12.11/8.11.7) with ESMTP id j95GvIVe025784 1, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 5 Oct 2005 12:57:18 -0400 (EDT) 1, 18 -- Mime-Version: 1.0 1, 18 -- X-Sender: rjpalmer-at-mail.nih.gov 1, 18 -- Message-Id: {p05200f00bf69b445c84a-at-[128.231.107.215]} 1, 18 -- Date: Wed, 5 Oct 2005 12:56:02 -0400 1, 18 -- To: Microscopy-at-Microscopy.Com 1, 18 -- From: "Robert J. Palmer Jr." {rjpalmer-at-dir.nidcr.nih.gov} 1, 18 -- Subject: TEM services 1, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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Title-Subject: [Filtered] MListserver:
Question: Fellow liststers,
A very grateful thanks to all who took the time to answer my query concerning the production of formvar coated grids. I saved them all for future reference! The two suggestions that got my Mojo working were using nose grease (my own) on the slide after cleaning it with EtOH and dipping the slide into the formvar solution and immediately withdrawing and drying it (instead of holding it in the solution for 10 sec and then in the vapor for 30 sec).
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Title-Subject: [Filtered] Need Video of Parasites for National Geographic
Question: Please respond to Video4TV-at-aol.com if you can help.
We are producing a program for National Geographic and are interested in licensing footage of the following:
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We apologize for the short notice but we would need to receive broadcast quality footage by this Friday, October 7. If anyone has this footage available for licensing, we would appreciate it!
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (distall2-at-yahoo.com) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, October 5, 2005 at 10:34:05 ---------------------------------------------------------------------------
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Question: what are the latest discovered microorganisms found with electron microscopes?
} Hi all, } } I was re-reading some catalogues of exhibits of Van Leeuwenhoek } microscopes, and I was struck, as usual, by the description of the } glass lenses that he made for his observations. These descriptions } reminded me of a comment that I overheard in passing at a recent M&M } meeting that Van Leeuwenhoek also made (or used) lenses made of } droplets of water. I know that he made a "water microscope", but I } always understood this to mean that the sample was liquid, contained } in a glass tube. Can anyone provide verification of the use of water } as a lens? } } Joel } } } } Joel
Roger Barker makes a fairly strong case that Van Leeuwenhoek made and used compound lenses for some of his drawings that appear to be both theoretically and practically impossible to resolve with the detail he drew with a single lens in www.science-info.org/pages/Roger%20Baker/homemade-microscope.pdf
He shows how he made a complex lens using a single lens of glass and one of glycerine.
Gordon Gordon Couger
I collect links on information related to light microscopes. www.couger.com/microscope/links/gclinks.html Please forward anything you think might be useful to others. Microscope Documentation is at www.science-info.org
==============================Original Headers============================== 9, 20 -- From gcc-at-couger.com Thu Oct 6 02:12:55 2005 9, 20 -- Received: from centrmmtao04.cox.net (centrmmtao04.cox.net [70.168.83.80]) 9, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j967Cs8Y003014 9, 20 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2005 02:12:54 -0500 9, 20 -- Received: from [127.0.0.1] (really [68.12.242.13]) by centrmmtao04.cox.net 9, 20 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 9, 20 -- id {20051006071240.TSJL20851.centrmmtao04.cox.net-at-[127.0.0.1]} ; 9, 20 -- Thu, 6 Oct 2005 03:12:40 -0400 9, 20 -- Message-ID: {4344CE88.7080403-at-couger.com} 9, 20 -- Date: Thu, 06 Oct 2005 02:13:12 -0500 9, 20 -- From: Gordon Couger {gcc-at-couger.com} 9, 20 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 9, 20 -- X-Accept-Language: en-us, en 9, 20 -- MIME-Version: 1.0 9, 20 -- To: jbs-at-temple.edu, microscopy-at-microscopy.com 9, 20 -- Subject: Re: [Microscopy] History - Van Leeuwenhoek Lenses 9, 20 -- References: {200510041444.j94Ei427014881-at-ns.microscopy.com} 9, 20 -- In-Reply-To: {200510041444.j94Ei427014881-at-ns.microscopy.com} 9, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
the discussion of Van Leeuwenhoek lenses raises a question of somewhat personal interest, since i will probably be looking at retiring from the paid part of the profession in 7-10 years. hey, it's never too early to think ahead when you have to drop really broad hints in the department.
some years ago we were looking at gifts for a compatriot who was leaving. we found one company, a purveyor of fine microscope slides for the student, which marketed silver Van Leeuwenhoek microscopes. the name was something like North Carolina Bio something, or South Carolina something-or-other.
does this ring a bell anywhere? can anyone tell me if such a thing still exists?
i mean, it's either going to be that or a nice Innu sculpture.
paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-3926
==============================Original Headers============================== 12, 17 -- From paul_hazelton-at-umanitoba.ca Thu Oct 6 09:24:24 2005 12, 17 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 12, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j96EOONB019421 12, 17 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2005 09:24:24 -0500 12, 17 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 12, 17 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id j96EOHwU008381; 12, 17 -- Thu, 6 Oct 2005 09:24:17 -0500 (CDT) 12, 17 -- Message-ID: {43453392.D941DA45-at-umanitoba.ca} 12, 17 -- Date: Thu, 06 Oct 2005 09:24:19 -0500 12, 17 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 12, 17 -- X-Mailer: Mozilla 4.78 [en] (Win98; U) 12, 17 -- X-Accept-Language: en,pdf 12, 17 -- MIME-Version: 1.0 12, 17 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 12, 17 -- Subject: Van Leeuwenhoek lenses and microscopes 12, 17 -- Content-Type: text/plain; charset=us-ascii 12, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
How about neither North nor South. Try http://www.carolina.com/. It looks like the place you might be looking for.
Warren
At 09:27 AM 10/06/05, you wrote:
} the discussion of Van Leeuwenhoek lenses raises a question of somewhat } personal interest, since i will probably be looking at retiring from the } paid part of the profession in 7-10 years. hey, it's never too early to } think ahead when you have to drop really broad hints in the department. } } some years ago we were looking at gifts for a compatriot who was } leaving. we found one company, a purveyor of fine microscope slides for } the student, which marketed silver Van Leeuwenhoek microscopes. the } name was something like North Carolina Bio something, or South Carolina } something-or-other. } } does this ring a bell anywhere? can anyone tell me if such a thing } still exists? } } i mean, it's either going to be that or a nice Innu sculpture. } } paul } } Paul R. Hazelton, PhD } Electron Microscope Unit } University of Manitoba } Department of Medical Microbiology
------------------------------------------- No files should be attached to this message ------------------------------------------- Warren E. Straszheim, Ph.D. Materials Analysis and Research Lab Iowa State University 46 Town Engineering Ames IA, 50011-3232
15 DAY AGO I HAVE LOST HEARING TOTALLY,in both ears. Was using a "In the Ear"(CIC) Digital Hearing Aid ( with Wax buster) Drs cannot find anything wrong with me Physicallly( was admitted to hospital for 4 days for all tests) While I have started work(just completed 25 yrs service),medicines are going in, still no improvemnet. Has been working more regularly, with the Jeol 5400 SEM during the past 3 months.Noticed extra battery dischage in the aid, and lower hearing capacity.Could it be a cause, of sudden loss of hearing for the last 3 months? Any of the MSA participants have had this experience? Kindly let me know, if there is a way out. Thanks & Regards,
J.J.D'Mello.
__________________________________ Yahoo! Mail - PC Magazine Editors' Choice 2005 http://mail.yahoo.com
==============================Original Headers============================== 4, 18 -- From j_dmello-at-yahoo.com Fri Oct 7 03:20:33 2005 4, 18 -- Received: from web34006.mail.mud.yahoo.com (web34006.mail.mud.yahoo.com [66.163.178.87]) 4, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j978KXMB016229 4, 18 -- for {microscopy-at-msa.microscopy.com} ; Fri, 7 Oct 2005 03:20:33 -0500 4, 18 -- Received: (qmail 97560 invoked by uid 60001); 7 Oct 2005 08:20:33 -0000 4, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 4, 18 -- s=s1024; d=yahoo.com; 4, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 4, 18 -- b=Uzd+xdCR68QbaJ9lCRpfWqiC7nZei+ByMU+gZrj3VkQb/mhuw/rnFsGJZQ4XIq8wGaXWM/tFln3HpnPQnDhQVguNuBXMJ1kZnXaIFXZwPzgPcYmUmQwLeu9FmAnB10KcxBEd77W8uQVLCCP+QqAPPDVH7BXSa0qL5OHO0VTPKk4= ; 4, 18 -- Message-ID: {20051007082033.97558.qmail-at-web34006.mail.mud.yahoo.com} 4, 18 -- Received: from [202.88.179.207] by web34006.mail.mud.yahoo.com via HTTP; Fri, 07 Oct 2005 01:20:32 PDT 4, 18 -- Date: Fri, 7 Oct 2005 01:20:32 -0700 (PDT) 4, 18 -- From: JUDE Dmello {j_dmello-at-yahoo.com} 4, 18 -- Subject: SEM Effects on Ear 4, 18 -- To: MicroscopyListserver {microscopy-at-msa.microscopy.com} 4, 18 -- MIME-Version: 1.0 4, 18 -- Content-Type: text/plain; charset=iso-8859-1 4, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Hello, I was given an old diamond knife. Former user does not know the specifications of this knife. On it's casing there is an engraving WR and underneath it MP309. I would like to know what kind of diamond knife it is (ultrathin, histo etc), is it 35 or 45 degree and what is it's cutting angle. If anybody can identify it I would be grateful for the information. Thanks Dorota
==============================Original Headers============================== 1, 24 -- From wadowska-at-avcn1.novell.upei.ca Fri Oct 7 06:56:53 2005 1, 24 -- Received: from mx.upei.ca (humboldt.cs.upei.ca [137.149.3.10]) 1, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j97Buohk027445 1, 24 -- for {microscopy-at-msa.microscopy.com} ; Fri, 7 Oct 2005 06:56:52 -0500 1, 24 -- Received: from [137.149.64.250] (helo=acad1.cs.upei.ca) 1, 24 -- by mx.upei.ca with esmtp (Exim 3.35 #1 (Debian)) 1, 24 -- id 1ENqqH-00025y-00 1, 24 -- for {microscopy-at-msa.microscopy.com} ; Fri, 07 Oct 2005 08:56:49 -0300 1, 24 -- Received: from AVCN1/SpoolDir by acad1.cs.upei.ca (Mercury 1.48); 1, 24 -- 7 Oct 05 08:56:49 -0300 1, 24 -- Received: from SpoolDir by AVCN1 (Mercury 1.48); 7 Oct 05 08:56:35 -0300 1, 24 -- From: "Dorota Wadowska" {wadowska-at-upei.ca} 1, 24 -- Organization: University of P.E.I. 1, 24 -- To: microscopy-at-msa.microscopy.com 1, 24 -- Date: Fri, 7 Oct 2005 08:53:52 -0400 1, 24 -- MIME-Version: 1.0 1, 24 -- Content-type: text/plain; charset=US-ASCII 1, 24 -- Content-transfer-encoding: 7BIT 1, 24 -- Subject: TEM diamond knife 1, 24 -- Message-ID: {434637A0.24440.2A2761-at-localhost} 1, 24 -- X-Confirm-Reading-To: "Dorota Wadowska" {wadowska-at-acad1.cs.upei.ca} 1, 24 -- X-pmrqc: 1 1, 24 -- Priority: normal 1, 24 -- X-mailer: Pegasus Mail for Win32 (v3.12c) ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (g.greaves-at-salford.student.ac.uk) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, October 7, 2005 at 05:23:35 ---------------------------------------------------------------------------
Question: I am trying to find information about the preparation of porous silicon samples to be analysed using a transmission electron microscope. What Techniques are there? any knowledge of papers relating to this would be greatly recieved.
I've been asked by two co-workers to provide purchasing advice for stereomicroscopes with digital image capture and continuous zoom of about 1 to 6 - ideally with options to increase/decrease magnification ranges.
One co-worker will be looking at opaque samples - usually rough cut to a few inches thick and sometimes further polished. The structures he's examining are varied shades of gray and black, thus lighting options, control, & reproducibility are important.
The other person will be looking at machined metal parts similar to long, thick needles with barbs on them. He's interested in measuring angles and other dimensions as well as seeing imperfections on the parts.
I have trinocular Olympus stereomicroscopes with a variety of lighting and lens options, and I'm happy with them for my research lab use. I've also checked out other companies' products via the web. But I'd like some real-world feedback on other stereomicroscopes - any you are thrilled with? any you would never buy again? any really good values for the money?
What are the current favorites for digital image capture - consumer, prosumer, & professional level digital cameras? I prefer at least 3 megapixels (more is always acceptable since the images can be downsized). Any comments on ease of use, quality, value, & satisfaction from recent purchasers?
Please respond directly to me at "Louise_Harner-at-albint.com" so we don't tie up the listserver with personal opinions. Vendor replies welcome, but please give pricing info. I need to get back to my co-workers by Oct. 14.
Thanks in advance!
- Louise
Louise Harner Research Microscopist, Albany International Research Co. 777 West Street, P.O. Box 9114, Mansfield, MA 02048 phone: 508-337-9529 Louise_Harner-at-albint.com
==============================Original Headers============================== 13, 19 -- From Louise_Harner-at-albint.com Fri Oct 7 08:13:51 2005 13, 19 -- Received: from alb-corp7.alb.amer.albint.com (smtp2.albint.com [24.105.170.136]) 13, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j97DDpSO013073 13, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 7 Oct 2005 08:13:51 -0500 13, 19 -- Importance: Normal 13, 19 -- X-Priority: 3 (Normal) 13, 19 -- Expiry-Date: 13, 19 -- Reply-To: 13, 19 -- Subject: advice on stereomicroscopes & cameras? 13, 19 -- Sensitivity: 13, 19 -- To: Microscopy-at-MSA.Microscopy.Com 13, 19 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 13, 19 -- Message-ID: {OF65A399B8.0EAE8E8F-ON85257091.0071A30C-85257092.006C6D57-at-albint.com} 13, 19 -- From: Louise_Harner-at-albint.com 13, 19 -- Date: Thu, 6 Oct 2005 15:44:18 -0400 13, 19 -- X-MIMETrack: Serialize by Router on ALB-CORP7/AlbInt(Release 6.5.4|March 27, 2005) at 13, 19 -- 2005-10-07 09:13:51 AM 13, 19 -- MIME-Version: 1.0 13, 19 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Does anyone have experience with using these tools for cross-sectional TEM specimen preparation? We are considering purchasing them and I am interested in opinions on the products. Please respond off-list if you have any comments for me.
Thank you, Leslie
Leslie Krupp (Thompson) IBM Almaden Research 650 Harry Road, K19/D2 San Jose, CA 95120-6099
==============================Original Headers============================== 4, 27 -- From lkrupp-at-us.ibm.com Fri Oct 7 09:45:33 2005 4, 27 -- Received: from e3.ny.us.ibm.com (e3.ny.us.ibm.com [32.97.182.143]) 4, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j97EjUli022548 4, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 09:45:32 -0500 4, 27 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236]) 4, 27 -- by e3.ny.us.ibm.com (8.12.11/8.12.11) with ESMTP id j97EjTOd001141 4, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 10:45:29 -0400 4, 27 -- Received: from d01av04.pok.ibm.com (d01av04.pok.ibm.com [9.56.224.64]) 4, 27 -- by d01relay04.pok.ibm.com (8.12.10/NCO/VERS6.7) with ESMTP id j97EjTGk067022 4, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 10:45:29 -0400 4, 27 -- Received: from d01av04.pok.ibm.com (loopback [127.0.0.1]) 4, 27 -- by d01av04.pok.ibm.com (8.12.11/8.13.3) with ESMTP id j97EjOmD025084 4, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 10:45:24 -0400 4, 27 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) 4, 27 -- by d01av04.pok.ibm.com (8.12.11/8.12.11) with ESMTP id j97EjNMV024171 4, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 10:45:24 -0400 4, 27 -- To: Microscopy-at-microscopy.com 4, 27 -- MIME-Version: 1.0 4, 27 -- Subject: polishing tools by Precision TEM, Inc 4, 27 -- X-Mailer: Lotus Notes Release 6.0.2CF1 June 9, 2003 4, 27 -- Message-ID: {OF6588FBDC.DDACC551-ON85257093.0050BB9B-88257093.0051084A-at-us.ibm.com} 4, 27 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} 4, 27 -- Date: Fri, 7 Oct 2005 07:45:05 -0700 4, 27 -- X-MIMETrack: Serialize by Router on D01ML604/01/M/IBM(Release 7.0HF2 | September 9, 2005) at 4, 27 -- 10/07/2005 10:45:23, 4, 27 -- Serialize complete at 10/07/2005 10:45:23 4, 27 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
I'm having troubles in recovering PT/C replicas on water from freshly cleaved mica. The carbon film remains sticked to the mica instead of floating on the water surface. Is there any trick to overcome this trouble ?
Thanks
Daniel
Daniel THOMAS Interactions Cellulaires et Moléculaires CNRS, UMR 6026 Université de Rennes 1 Campus de Beaulieu, Bt 13 35042 RENNES Cedex
Tel : 02 23 23 6122 fax : 02 23 23 5048
http://www.sdm.univ-rennes1.fr/
==============================Original Headers============================== 19, 26 -- From daniel.thomas-at-univ-rennes1.fr Fri Oct 7 10:31:50 2005 19, 26 -- Received: from mailimailo.univ-rennes1.fr (mailimailo.univ-rennes1.fr [129.20.131.1]) 19, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j97FVnfW031465 19, 26 -- for {Microscopy-at-Microscopy.Com} ; Fri, 7 Oct 2005 10:31:49 -0500 19, 26 -- Received: from localhost (localhost [127.0.0.1]) 19, 26 -- by mailimailo.univ-rennes1.fr (Postfix) with ESMTP id 3177F4077 19, 26 -- for {Microscopy-at-Microscopy.Com} ; Fri, 7 Oct 2005 17:31:49 +0200 (MEST) 19, 26 -- Received: from mailimailo.univ-rennes1.fr ([127.0.0.1]) 19, 26 -- by localhost (mailimailo.univ-rennes1.fr [127.0.0.1]) (amavisd-new, port 10024) 19, 26 -- with LMTP id 27615-01-45 for {Microscopy-at-Microscopy.Com} ; 19, 26 -- Fri, 7 Oct 2005 17:31:45 +0200 (MEST) 19, 26 -- Received: from pc-dthomas-port.univ-rennes1.fr (pc-dthomas-port.sve.univ-rennes1.fr [129.20.85.160]) 19, 26 -- by mailimailo.univ-rennes1.fr (Postfix) with ESMTP id 93BEB4107 19, 26 -- for {Microscopy-at-Microscopy.Com} ; Fri, 7 Oct 2005 17:31:45 +0200 (MEST) 19, 26 -- Message-Id: {5.1.0.14.0.20051007173019.02d71970-at-mailhost.univ-rennes1.fr} 19, 26 -- X-Sender: dthomas-at-mailhost.univ-rennes1.fr 19, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 5.1 19, 26 -- Date: Fri, 07 Oct 2005 17:31:44 +0200 19, 26 -- To: Microscopy-at-Microscopy.Com 19, 26 -- From: Daniel Thomas {daniel.thomas-at-univ-rennes1.fr} 19, 26 -- Subject: TEM :recovering replicas from mica 19, 26 -- Mime-Version: 1.0 19, 26 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 19, 26 -- X-Virus-Scanned: by amavisd-new at univ-rennes1.fr 19, 26 -- Content-Transfer-Encoding: 8bit 19, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j97FVnfW031465 ==============================End of - Headers==============================
We have some paraffin sections on glass slides (silane coated, ems slides) that we would need to embed into Spurr's resin.
Does anyone have a suggestion on how to separate the glass from the Spurr's resin blocks? We briefly plunged them into liquid nitrogen, but we did not get consistent results.
Also, shall we use a different resin?
Thank you.
Tea Meulia
-- *************************************** Tea Meulia Molecular and Cellular Imaging Center Ohio State University/OARDC 1680 Madison Ave. Wooster OH 44691
tel.: 330-263-3836 or -3828 fax: 330-202-3563
http://www.oardc.ohio-state.edu/mcic
*****************************************
==============================Original Headers============================== 10, 18 -- From meulia.1-at-osu.edu Fri Oct 7 11:03:12 2005 10, 18 -- Received: from defang20.it.ohio-state.edu (defang20.it.ohio-state.edu [128.146.216.134]) 10, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j97G3BHK007963 10, 18 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 11:03:12 -0500 10, 18 -- Received: from [164.107.84.190] (dhcp-107-84-190.oardc.ohio-state.edu [164.107.84.190]) 10, 18 -- by defang20.it.ohio-state.edu (8.13.1/8.13.1) with ESMTP id j97G3BIJ021917 10, 18 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 12:03:11 -0400 10, 18 -- Mime-Version: 1.0 10, 18 -- X-Sender: meulia.1-at-pop.service.ohio-state.edu 10, 18 -- Message-Id: {p05200f07bf6c481aad81-at-[164.107.84.190]} 10, 18 -- Date: Fri, 7 Oct 2005 12:06:55 -0400 10, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 10, 18 -- From: Tea Meulia {meulia.1-at-osu.edu} 10, 18 -- Subject: paraffin sections embedding into plastic 10, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 10, 18 -- X-Spam-Score: undef - spam scanning disabled 10, 18 -- X-CanItPRO-Stream: outbound 10, 18 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 128.146.216.12 ==============================End of - Headers==============================
On Oct 7, 2005, at 8:31 AM, daniel.thomas-at-univ-rennes1.fr wrote:
} TEM : PT/C replicas of biological molecules. } } I'm having troubles in recovering PT/C replicas on water from freshly } cleaved mica. The carbon film remains sticked to the mica instead of } floating on the water surface. Is there any trick to overcome this } trouble ?
Dear Daniel, The time I had problems with removing C films from mica, the humidity was higher than usual. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 28 -- From tivol-at-caltech.edu Fri Oct 7 11:37:28 2005 6, 28 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j97GbSwJ016836 6, 28 -- for {microscopy-at-msa.microscopy.com} ; Fri, 7 Oct 2005 11:37:28 -0500 6, 28 -- Received: from localhost (water-dog [192.168.1.26]) 6, 28 -- by water-ox-postvirus (Postfix) with ESMTP id 3CAA2339F3 6, 28 -- for {microscopy-at-msa.microscopy.com} ; Fri, 7 Oct 2005 09:37:28 -0700 (PDT) 6, 28 -- Received: from earth-ox ([192.168.1.9]) 6, 28 -- by water-dog (MailMonitor for SMTP v1.2.2 ) ; 6, 28 -- Fri, 7 Oct 2005 09:37:27 -0700 (PDT) 6, 28 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 6, 28 -- by earth-ox.its.caltech.edu (Postfix) with ESMTP id 11B30109A25 6, 28 -- for {microscopy-at-msa.microscopy.com} ; Fri, 7 Oct 2005 09:37:27 -0700 (PDT) 6, 28 -- Mime-Version: 1.0 (Apple Message framework v623) 6, 28 -- In-Reply-To: {200510071531.j97FVwkX031645-at-ns.microscopy.com} 6, 28 -- References: {200510071531.j97FVwkX031645-at-ns.microscopy.com} 6, 28 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 6, 28 -- Message-Id: {253a9c0be36246dd63f342ed7571c359-at-caltech.edu} 6, 28 -- Content-Transfer-Encoding: 7bit 6, 28 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 28 -- Subject: Re: [Microscopy] TEM :recovering replicas from mica 6, 28 -- Date: Fri, 7 Oct 2005 09:40:54 -0700 6, 28 -- To: microscopy-at-msa.microscopy.com 6, 28 -- X-Mailer: Apple Mail (2.623) 6, 28 -- X-Spam-Status: No, hits=-6.9 tagged_above=-10000 required=5 6, 28 -- tests=[ALL_TRUSTED=-3.8, CIT_FROM_ADDR=-0.7, CIT_NET_FROM=-3.9, 6, 28 -- FS_REPLICA=1.5] 6, 28 -- X-Spam-Level: ==============================End of - Headers==============================
I purchased several stereomicroscopes in my career. It was always relatively easy to ask a local representative of the major brands to bring a unit in for a day or in some cases, a week to try it out with doing the things that you do with it. It was surprising to me how different each brand was from one to another in how it felt using it and how comfortable I was the different units. My suggestion is to make some calls and book some appointments with your local reps and see what the consensus is among the different users in the lab after they have had some hands on with each unit.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: Louise_Harner-at-albint.com [mailto:Louise_Harner-at-albint.com] Sent: Friday, October 07, 2005 6:19 AM To: Walck-at-SouthBayTech.com
I've been asked by two co-workers to provide purchasing advice for stereomicroscopes with digital image capture and continuous zoom of about 1 to 6 - ideally with options to increase/decrease magnification ranges.
One co-worker will be looking at opaque samples - usually rough cut to a few inches thick and sometimes further polished. The structures he's examining are varied shades of gray and black, thus lighting options, control, & reproducibility are important.
The other person will be looking at machined metal parts similar to long, thick needles with barbs on them. He's interested in measuring angles and other dimensions as well as seeing imperfections on the parts.
I have trinocular Olympus stereomicroscopes with a variety of lighting and lens options, and I'm happy with them for my research lab use. I've also checked out other companies' products via the web. But I'd like some real-world feedback on other stereomicroscopes - any you are thrilled with? any you would never buy again? any really good values for the money?
What are the current favorites for digital image capture - consumer, prosumer, & professional level digital cameras? I prefer at least 3 megapixels (more is always acceptable since the images can be downsized). Any comments on ease of use, quality, value, & satisfaction from recent purchasers?
Please respond directly to me at "Louise_Harner-at-albint.com" so we don't tie up the listserver with personal opinions. Vendor replies welcome, but please give pricing info. I need to get back to my co-workers by Oct. 14.
Thanks in advance!
- Louise
Louise Harner Research Microscopist, Albany International Research Co. 777 West Street, P.O. Box 9114, Mansfield, MA 02048 phone: 508-337-9529 Louise_Harner-at-albint.com
==============================Original Headers============================== 13, 19 -- From Louise_Harner-at-albint.com Fri Oct 7 08:13:51 2005 13, 19 -- Received: from alb-corp7.alb.amer.albint.com (smtp2.albint.com [24.105.170.136]) 13, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j97DDpSO013073 13, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 7 Oct 2005 08:13:51 -0500 13, 19 -- Importance: Normal 13, 19 -- X-Priority: 3 (Normal) 13, 19 -- Expiry-Date: 13, 19 -- Reply-To: 13, 19 -- Subject: advice on stereomicroscopes & cameras? 13, 19 -- Sensitivity: 13, 19 -- To: Microscopy-at-MSA.Microscopy.Com 13, 19 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 13, 19 -- Message-ID: {OF65A399B8.0EAE8E8F-ON85257091.0071A30C-85257092.006C6D57-at-albint.com} 13, 19 -- From: Louise_Harner-at-albint.com 13, 19 -- Date: Thu, 6 Oct 2005 15:44:18 -0400 13, 19 -- X-MIMETrack: Serialize by Router on ALB-CORP7/AlbInt(Release 6.5.4|March 27, 2005) at 13, 19 -- 2005-10-07 09:13:51 AM 13, 19 -- MIME-Version: 1.0 13, 19 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
==============================Original Headers============================== 23, 27 -- From walck-at-southbaytech.com Fri Oct 7 11:41:09 2005 23, 27 -- Received: from ylpvm12.prodigy.net (ylpvm12-ext.prodigy.net [207.115.57.43]) 23, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j97Gf990023408 23, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 11:41:09 -0500 23, 27 -- Received: from ylpvm01.prodigy.net (ylpvm01-int.prodigy.net [207.115.5.207]) 23, 27 -- by ylpvm12.prodigy.net (8.12.10 outbound/8.12.10) with ESMTP id j97Geurk016303 23, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 12:40:58 -0400 23, 27 -- X-ORBL: [64.169.193.90] 23, 27 -- Received: from dynamicbl8uno3 (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90]) 23, 27 -- by ylpvm01.prodigy.net (8.13.4 dk-milter linux/8.13.4) with ESMTP id j97GeJdk016446; 23, 27 -- Fri, 7 Oct 2005 12:40:20 -0400 23, 27 -- From: "Scott Walck" {walck-at-southbaytech.com} 23, 27 -- To: "MicroscopyListserver" {Microscopy-at-microscopy.com} 23, 27 -- Cc: {Louise_Harner-at-albint.com} 23, 27 -- Subject: RE: [Microscopy] advice on stereomicroscopes & cameras? 23, 27 -- Date: Fri, 7 Oct 2005 09:41:36 -0700 23, 27 -- Message-ID: {001d01c5cb5d$fc235c20$04000100-at-dynamicbl8uno3} 23, 27 -- MIME-Version: 1.0 23, 27 -- Content-Type: text/plain; 23, 27 -- charset="us-ascii" 23, 27 -- Content-Transfer-Encoding: 7bit 23, 27 -- X-Priority: 3 (Normal) 23, 27 -- X-MSMail-Priority: Normal 23, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 23, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 23, 27 -- Importance: Normal 23, 27 -- In-Reply-To: {200510071318.j97DIXSp019827-at-ns.microscopy.com} ==============================End of - Headers==============================
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Have you actually tried any standard methods yet? And if so, what were the problems? What is the initial form of your samples? How are the pores created and what size are they?? Are the samples amorphous, polycrystalline or single crystal? If single crystal, what orientation?
I don't have personal experience of this material but methods I would try include:
1. Mechanical pre-preparation - diamond saw, ultrasonic disc cutter, grinding and polishing to get 3 mm discs, ~500 um thick, dimple grinder then ion thin.
2. If the ion thinning destroys the pore structure, then it may be possible to mechnically polish with a dimple grinder to electron transparency. Si becomes transparent to light when very thin. This can be used to control the final dimple polishing.
3. Easiest method, if pores are small, might be to break and grind the Si until very fine, disperse in ethanol and dry down onto a C support film on a grid.
PLEASE NOTE 1. Any mail other than plain text will be automatically deleted. 2. Any mail, legitimate or not, apparently or actually from hotmail, netscape, yahoo or excite will automatically be deleted. 3. Mail with no subject or without a clear subject will be ignored :-)
==============================Original Headers============================== 8, 16 -- From larry-at-cymru.freewire.co.uk Fri Oct 7 11:45:23 2005 8, 16 -- Received: from get.freewire.net (get.freewire.net [195.184.229.250]) 8, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j97GjMiS000598 8, 16 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 7 Oct 2005 11:45:22 -0500 8, 16 -- Received: from [217.154.254.154] ([217.154.254.154]) 8, 16 -- by get.freewire.net (8.11.6/8.11.6) with ESMTP id j97GjIF02253; 8, 16 -- Fri, 7 Oct 2005 17:45:19 +0100 8, 16 -- Mime-Version: 1.0 8, 16 -- Message-Id: {p06210200bf6c527ebfec-at-[217.154.250.72]} 8, 16 -- In-Reply-To: {200510071247.j97Cl5rI006632-at-ns.microscopy.com} 8, 16 -- References: {200510071247.j97Cl5rI006632-at-ns.microscopy.com} 8, 16 -- Date: Fri, 7 Oct 2005 17:41:23 +0100 8, 16 -- To: g.greaves-at-salford.student.ac.uk, Microscopy-at-MSA.Microscopy.Com 8, 16 -- From: Larry Stoter {larry-at-cymru.freewire.co.uk} 8, 16 -- Subject: Re: [Microscopy] AskAMicroscopist:TEM of porous silicon samples 8, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
unfortunately at the moment I can't find the respective file dealing with the } POP-OFF {-technique I used for long time earlier(1980-1990ies), so I would like to inform you of a website-document you can read after for this "technique".
You can find this paper at http://www.ebsciences.com/papers/large_block.htm (scrolling down to the section "POP-OFF-Technique"). For your convenience I have pasted in here the text:
citation } A size 3 BEEM capsule is filled with liquid resin until it is slightly concave. The capsule is inverted quickly, placed over the etched/marked area, and polymerized according to the plastic used. Lowicryl sections pop off best with a resin mixture using half the amount of BEE. Polymerized capsules are popped off the glass slides by dipping them quickly in and out of a dewar containing liquid nitrogen (they must not be frozen too long). Capsules will be easily lifted off. The pop-off capsules are stained in 1.0% methylene blue at room temperature for 30 to 60 seconds, rinsed in distilled water, and dried. The capsules are examined under a light microscope (condenser down) to determine the flatness of the etched tissue section embedded in the pop-off's surface. If the etched area is uniformly stained and appears in one plane of focus, it is suitable for thin sectioning. { {End of quote
The separation of (WELL/THOROUGHLY impregnated [former] paraffin sections reeembedded into and then polymerized resin [IMO it doesn't matter if epoxy or acrylic one's] everytimes in my experience was tricky and not every section/slide behaved in a same/similar manner concerning separation (tendencies).....
Usually, you will have to mount an empty resin block over the section at the location desired (I never heard or read about separating a whole section from the glass-slide without decreasing area and mounting - if you like - one or more blank resin blocks on the slide). After polymerisation of the blocks in position over the chosen location you better scratch the edges around the block-section contact with a sharp scalpel.
The "trick" is - IMO - NOT to plunge the whole slide INTO liquid nitrogen, BUT -instead - only to place the slide e.g. 1 mm ABOVE the LN2, and finally (after 1-2 seconds of having done so to reduce thermal capacity of the glass slide) to get the slide with its lower surface ON to the LN2 surface.
You will see "freezing" of the slide (last time point for you: when the thicker edges of the mounted resin blocks get white !): THEN: either you will "hear" a "pop" (this } popping { gave the name to the technique) or not: if the latter occurs, try to push the block by your fingers in a +/- horizontal direction. Sometimes it is necessary to try that procedure several times.
If you don't have a positive result (a likely result for silane coated slides), try - as a last trial for rescue - the following: before performing the pop-off technique on LN2 surface, place the slide with its resin blocks mounted into the oven at -say- 90 degrees C for 15 min or so, then transfer as quickly as possible to the cold LN2 surface....
Physical Mechanism (as to my knowledge): it is clear that the different thermal capacity of the glass slide and the resin blocks/section on the other hand will result in different expansion properties (extension modulus) if cooled down to - 196 degrees C. Therefore it is necessary to get a very cold glass slide, but instead a more/less "warm" resin block boundary of the reembedded section.
Hope this helps, Best wishes and regards
Wolfgang Muss
Personal Communication Data: OR Dr. Wolfgang Muss Member of EM-Lab Institute of Pathology, SALK (Salzburger Landeskliniken gemeinnuetzige GesmbH) Muellner Hauptstrasse 48 A-5020 SALZBURG AUSTRIA/EUROPE
and/or/alternatively
Paracelsus Medical Private University (PMU) Institute of Pathology Electron Microscopy Lab Muellner Hauptstrasse 48 A-5020 SALZBURG, Austria/Europe Phone work: +43+662+4482+4720 Mobile phone work:+43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss") E-Mail work: W.Muss-at-SALK.at
Mobile-phone private: ++43+676+5 369-456 E-Mail private: wij.Muss-at-aon.at ------------------------------------------------------------------------ -------------------------------------- Ankuendigung namens der (Information on behalf of) Society for Cutaneous Ultrastructure Research (SCUR) PLEASE VISIT THE UPDATED WEBSITE of SCUR at } http://www.scur.org { ------------------------------------------------------------------------- Forthcoming Meetings: 33rd Annual Meeting of the SCUR, 8th-10th June, 2006, WARSZAW, Poland Preliminary informations: send an E-Mail kwoznia-at-amwaw.edu.pl
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We have some paraffin sections on glass slides (silane coated, ems slides) that we would need to embed into Spurr's resin.
Does anyone have a suggestion on how to separate the glass from the Spurr's resin blocks? We briefly plunged them into liquid nitrogen, but we did not get consistent results.
Also, shall we use a different resin?
Thank you.
Tea Meulia
-- *************************************** Tea Meulia Molecular and Cellular Imaging Center Ohio State University/OARDC 1680 Madison Ave. Wooster OH 44691
tel.: 330-263-3836 or -3828 fax: 330-202-3563
http://www.oardc.ohio-state.edu/mcic
*****************************************
==============================Original Headers============================== 10, 18 -- From meulia.1-at-osu.edu Fri Oct 7 11:03:12 2005 10, 18 -- Received: from defang20.it.ohio-state.edu (defang20.it.ohio-state.edu [128.146.216.134]) 10, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j97G3BHK007963 10, 18 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 11:03:12 -0500 10, 18 -- Received: from [164.107.84.190] (dhcp-107-84-190.oardc.ohio-state.edu [164.107.84.190]) 10, 18 -- by defang20.it.ohio-state.edu (8.13.1/8.13.1) with ESMTP id j97G3BIJ021917 10, 18 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 12:03:11 -0400 10, 18 -- Mime-Version: 1.0 10, 18 -- X-Sender: meulia.1-at-pop.service.ohio-state.edu 10, 18 -- Message-Id: {p05200f07bf6c481aad81-at-[164.107.84.190]} 10, 18 -- Date: Fri, 7 Oct 2005 12:06:55 -0400 10, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 10, 18 -- From: Tea Meulia {meulia.1-at-osu.edu} 10, 18 -- Subject: paraffin sections embedding into plastic 10, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 10, 18 -- X-Spam-Score: undef - spam scanning disabled 10, 18 -- X-CanItPRO-Stream: outbound 10, 18 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 128.146.216.12 ==============================End of - Headers==============================
==============================Original Headers============================== 38, 28 -- From W.Muss-at-salk.at Fri Oct 7 11:54:33 2005 38, 28 -- Received: from n1fm211.lks.local (hermes.lks.at [193.170.167.9]) 38, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j97GsWkl010064 38, 28 -- for {Microscopy-at-Microscopy.Com} ; Fri, 7 Oct 2005 11:54:32 -0500 38, 28 -- Received: from localhost (localhost [127.0.0.1]) 38, 28 -- by hermes.lks.at (Postfix) with ESMTP id 28D4A5A9035; 38, 28 -- Fri, 7 Oct 2005 18:54:30 +0200 (CEST) 38, 28 -- Received: from hermes.lks.at ([127.0.0.1]) 38, 28 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024) 38, 28 -- with ESMTP id 97296-09; Fri, 7 Oct 2005 18:54:29 +0200 (CEST) 38, 28 -- Received: from c1pa003 (unknown [192.168.42.3]) 38, 28 -- by hermes.lks.at (Postfix) with SMTP id 79D785A9031; 38, 28 -- Fri, 7 Oct 2005 18:54:29 +0200 (CEST) 38, 28 -- Received: by localhost with Microsoft MAPI; Fri, 7 Oct 2005 18:54:20 +0200 38, 28 -- Message-ID: {01C5CB70.86C28D80.W.Muss-at-salk.at} 38, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at} 38, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at} 38, 28 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-Microscopy.Com} 38, 28 -- Cc: "'meulia.1-at-osu.edu'" {meulia.1-at-osu.edu} 38, 28 -- Subject: AW: [Microscopy] paraffin sections embedding into plastic 38, 28 -- Date: Fri, 7 Oct 2005 18:54:19 +0200 38, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at} 38, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor 38, 28 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211 38, 28 -- MIME-Version: 1.0 38, 28 -- Content-Type: text/plain; charset="us-ascii" 38, 28 -- Content-Transfer-Encoding: 7bit 38, 28 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at ==============================End of - Headers==============================
Tea, Separating sections from plain glass slides, or even the old fashioned albumin or gelatin coated slides usually worked pretty well using the liq. nitrogen method. Silanized slides (or "plus" slides) seem to be a whole 'nother animal. The treatment is designed to keep the sections on the slide...and it does. I suppose you could try etching away the glass with HF...but that is very dangerous and not a step to take lightly. If you do go that route, I would use a diamond scribe to etch and break the slide as close to the block face at possible in order to minimize both the amount of HF needed, and the time to dissolve the glass. If you think you need to use HF, contact me off-list and I will regale you with precautions to take. Lee
-- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175
==============================Original Headers============================== 4, 22 -- From lcgould-at-med.cornell.edu Fri Oct 7 12:06:47 2005 4, 22 -- Received: from smtp-in2.med.cornell.edu (smtp-in2.med.cornell.edu [140.251.0.25]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j97H6kk7018668 4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 12:06:47 -0500 4, 22 -- Received: from mpx1.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) 4, 22 -- by smtp-in2.med.cornell.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id j97H6eM1205442 4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 13:06:45 -0400 4, 22 -- Received: from [140.251.145.131] by mpx1.med.cornell.edu 4, 22 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 4, 22 -- with ESMTP id {0IO000E15272P180-at-mpx1.med.cornell.edu} for 4, 22 -- microscopy-at-microscopy.com; Fri, 07 Oct 2005 13:06:40 -0400 (EDT) 4, 22 -- Date: Fri, 07 Oct 2005 13:06:36 -0400 4, 22 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 4, 22 -- Subject: Re: [Microscopy] paraffin sections embedding into plastic 4, 22 -- In-reply-to: {200510071604.j97G45jx009289-at-ns.microscopy.com} 4, 22 -- X-Sender: lcgould-at-pop.med.cornell.edu 4, 22 -- To: meulia.1-at-osu.edu, Microscopy Listserver {microscopy-at-microscopy.com} 4, 22 -- Message-id: {p06020402bf6c5a20d4ef-at-[140.251.145.131]} 4, 22 -- MIME-version: 1.0 4, 22 -- Content-type: text/plain; charset=us-ascii; format=flowed 4, 22 -- References: {200510071604.j97G45jx009289-at-ns.microscopy.com} 4, 22 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.1.0.0, Antispam-Data: 2005.10.7.18 ==============================End of - Headers==============================
I would definitely add the small angle cleavage technique to the standard technique (aka MicroCleave(TM) technique) for this. If you need information on this technique, please visit our website and look up application notes associated with the Model 520.
I've seen cross sections of porous silicon samples in the literature and it didn't appear that they had done anything different. The epoxies that are used should infiltrate the porous silicon fairly well. You should be able to use ay of the standard techniques for cross section sample prep. A number of application notes are available on our web site that describe these techniques.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
Disclaimer: South Bay Technology manufactures and sells the MicroCleave(TM) and the full complement of TEM sample preparation equipment.
-----Original Message----- X-from: larry-at-cymru.freewire.co.uk [mailto:larry-at-cymru.freewire.co.uk] Sent: Friday, October 07, 2005 9:49 AM To: Walck-at-SouthBayTech.com
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Have you actually tried any standard methods yet? And if so, what were the problems? What is the initial form of your samples? How are the pores created and what size are they?? Are the samples amorphous, polycrystalline or single crystal? If single crystal, what orientation?
I don't have personal experience of this material but methods I would try include:
1. Mechanical pre-preparation - diamond saw, ultrasonic disc cutter, grinding and polishing to get 3 mm discs, ~500 um thick, dimple grinder then ion thin.
2. If the ion thinning destroys the pore structure, then it may be possible to mechnically polish with a dimple grinder to electron transparency. Si becomes transparent to light when very thin. This can be used to control the final dimple polishing.
3. Easiest method, if pores are small, might be to break and grind the Si until very fine, disperse in ethanol and dry down onto a C support film on a grid.
PLEASE NOTE 1. Any mail other than plain text will be automatically deleted. 2. Any mail, legitimate or not, apparently or actually from hotmail, netscape, yahoo or excite will automatically be deleted. 3. Mail with no subject or without a clear subject will be ignored :-)
==============================Original Headers============================== 8, 16 -- From larry-at-cymru.freewire.co.uk Fri Oct 7 11:45:23 2005 8, 16 -- Received: from get.freewire.net (get.freewire.net [195.184.229.250]) 8, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j97GjMiS000598 8, 16 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 7 Oct 2005 11:45:22 -0500 8, 16 -- Received: from [217.154.254.154] ([217.154.254.154]) 8, 16 -- by get.freewire.net (8.11.6/8.11.6) with ESMTP id j97GjIF02253; 8, 16 -- Fri, 7 Oct 2005 17:45:19 +0100 8, 16 -- Mime-Version: 1.0 8, 16 -- Message-Id: {p06210200bf6c527ebfec-at-[217.154.250.72]} 8, 16 -- In-Reply-To: {200510071247.j97Cl5rI006632-at-ns.microscopy.com} 8, 16 -- References: {200510071247.j97Cl5rI006632-at-ns.microscopy.com} 8, 16 -- Date: Fri, 7 Oct 2005 17:41:23 +0100 8, 16 -- To: g.greaves-at-salford.student.ac.uk, Microscopy-at-MSA.Microscopy.Com 8, 16 -- From: Larry Stoter {larry-at-cymru.freewire.co.uk} 8, 16 -- Subject: Re: [Microscopy] AskAMicroscopist:TEM of porous silicon samples 8, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 20, 26 -- From walck-at-southbaytech.com Fri Oct 7 12:54:54 2005 20, 26 -- Received: from ylpvm15.prodigy.net (ylpvm15-ext.prodigy.net [207.115.57.46]) 20, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j97Hsrma027732 20, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 12:54:53 -0500 20, 26 -- Received: from pimout7-ext.prodigy.net (pimout7-int.prodigy.net [207.115.4.147]) 20, 26 -- by ylpvm15.prodigy.net (8.12.10 outbound/8.12.10) with ESMTP id j97Ht1KE032449 20, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 13:55:01 -0400 20, 26 -- X-ORBL: [64.169.193.90] 20, 26 -- Received: from dynamicbl8uno3 (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90]) 20, 26 -- by pimout7-ext.prodigy.net (8.13.4 outbound domainkey aix/8.13.4) with ESMTP id j97HskIq103994 20, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 13:54:51 -0400 20, 26 -- From: "Scott Walck" {walck-at-southbaytech.com} 20, 26 -- To: "MicroscopyListserver" {Microscopy-at-microscopy.com} 20, 26 -- Subject: RE: [Microscopy] Re: AskAMicroscopist:TEM of porous silicon samples 20, 26 -- Date: Fri, 7 Oct 2005 10:54:46 -0700 20, 26 -- Message-ID: {002801c5cb68$3780c370$04000100-at-dynamicbl8uno3} 20, 26 -- MIME-Version: 1.0 20, 26 -- Content-Type: text/plain; 20, 26 -- charset="us-ascii" 20, 26 -- Content-Transfer-Encoding: 7bit 20, 26 -- X-Priority: 3 (Normal) 20, 26 -- X-MSMail-Priority: Normal 20, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 20, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 20, 26 -- Importance: Normal 20, 26 -- In-Reply-To: {200510071648.j97Gmx1d008197-at-ns.microscopy.com} ==============================End of - Headers==============================
To be held in conjunction with the March 24, 2006 meeting of the Midwest
Microscopy and Microanalysis Society
Affiliate of the Microscopy Society of America and the Microbeam Analysis Society of America
ABSTRACT DEADLINE: Abstracts must be received in electronic format by 5PM, Friday, December 16, 2005.
The first quarter meeting of the Midwest Microscopy and Microanalysis Society will be held on Friday, March 24, 2006, in the Pancoe Life Sciences Building, Northwestern University, Evanston, Illinois. A student poster
competition open to undergraduate and graduate students will be held in conjunction with the meeting. Posters should illustrate utilization of microscopy for either biological or materials science study. Prizes will be awarded as follows:
$300 for 1st place $200 for 2nd place $100 for 3rd place
Abstracts must be received in electronic format by 5PM on Friday, December 16, 2005. To be eligible for a prize, you must be first author on the poster, and you must be present at the meeting. You are encouraged to submit your entry as early as possible, as space may be limited. Abstracts from last year's competition, and an example of the judging worksheet can be found on the MMMS website:
Dear Daniel: We routinely remove Pt/C replicas or shadowed samples of polymers, from mica or glass cover slips, after also coating with vertically coated C, by floating on dilute (ca 1%) HF, taking all the necessary HF use precautions. The sample can be picked up on the grid, and either touched to a paper tissue or refloated on water to remove any residual HF. If any questions let me know. Regards, Phil Geil --
Phillip H. Geil; Ph. 217-333-0149 Fax 217-333-2736 Department of Materials Science and Engineering University of Illinois 1304 W. Green St. Urbana, IL 61801
==============================Original Headers============================== 3, 14 -- From geil-at-uiuc.edu Fri Oct 7 15:50:06 2005 3, 14 -- Received: from expredir6.cites.uiuc.edu (expredir6.cites.uiuc.edu [128.174.5.97]) 3, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j97Ko5cs014547 3, 14 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 15:50:05 -0500 3, 14 -- Received: from [128.174.228.35] (mach-35.mse.uiuc.edu [128.174.228.35]) 3, 14 -- by expredir6.cites.uiuc.edu (8.12.11/8.12.11) with ESMTP id j97KndvR007242 3, 14 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Oct 2005 15:49:40 -0500 (CDT) 3, 14 -- Mime-Version: 1.0 3, 14 -- Message-Id: {p06230905bf6c87b7395a-at-[128.174.228.35]} 3, 14 -- Date: Fri, 7 Oct 2005 15:40:03 -0500 3, 14 -- To: Microscopy-at-microscopy.com 3, 14 -- From: Phillip Geil {geil-at-uiuc.edu} 3, 14 -- Subject: TEM, replica removal 3, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
If you have used an electrostatic glue (i.e. poly-L-lysine) the replica will NOT separate from the mica without using HF (in my experience). If you do use HF to remove the replica, I would recommend using a glass rod (yes, glass) to remove the replica to distilled water before picking up on a grid. You just touch the rod to the surface of the liquid and "roll" the replica up onto the rod and "unroll" it onto the surface of the water. I feel this minimizes the stress to the replica. This method was mentioned in a paper by John Heuser but unfortunately I don't have the citation for you. (I made a platinum/carbon replica on a poly-L-lysine-coated glass coverslip and removed the replica onto full-strength HF just a few hours ago, so I know that works!)
On an HF-free note, in the book "Negative Staining and Cryoelectron Microscopy", Robin Harris recommends letting carbon films evaporated onto mica sit overnight before attempting to float them off onto water. As a possible means to avoid waiting overnight, Harris suggests placing the mica into a petri dish on some damp filter papers for a few hours. Perhaps this might be enough to get your replicas to float.
Good luck!
Andrew Bowling post-PhD but pre-Postdoc The University of Texas Austin, Texas
==============================Original Headers============================== 6, 17 -- From abowling-at-mail.utexas.edu Sat Oct 8 00:08:12 2005 6, 17 -- Received: from wb2-a.mail.utexas.edu (wb2-a.mail.utexas.edu [128.83.126.136]) 6, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9858CtD027477 6, 17 -- for {Microscopy-at-microscopy.com} ; Sat, 8 Oct 2005 00:08:12 -0500 6, 17 -- Received: (qmail 27991 invoked from network); 8 Oct 2005 05:08:12 -0000 6, 17 -- Received: from 216-188-252-161.dyn.grandenetworks.net (HELO ?192.168.0.3?) (abowling-at-216.188.252.161) 6, 17 -- by wb2.mail.utexas.edu with RC4-MD5 encrypted SMTP; 8 Oct 2005 05:08:12 -0000 6, 17 -- Message-ID: {43475433.6080907-at-mail.utexas.edu} 6, 17 -- Date: Sat, 08 Oct 2005 00:08:03 -0500 6, 17 -- From: Andrew Bowling {abowling-at-mail.utexas.edu} 6, 17 -- User-Agent: Mozilla Thunderbird 1.0.6 (X11/20050716) 6, 17 -- X-Accept-Language: en-us, en 6, 17 -- MIME-Version: 1.0 6, 17 -- To: Microscopy-at-microscopy.com 6, 17 -- Subject: Re: TEM: recovering replicas from mica 6, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Generally micro-organisms aren't discovered using the electron microsope. They are normally taken from their natural habitat, such as soil, the air, blood and so forth, and may be grown ('cultured') in special media that contains the nutrients they need to survive. The media may be an Agar plate (a jelly or jello like material ) or a soupy broth liquid. The single celled organisms divide in this producing millions of cells (and hopefully all of the type you want). Viruses are more complicated as they require a cell to replicate in. Once there are plenty of micro-organisms in the culture they can be harvested and treated for viewing under the light or electron microscope. Light microscopy is, with the use of special cellular stains, is still very important for micro-organism identification. Once isolated and cultured we can also study them to see how they live and how to kill them if they are dangerous.
So normally the micro-organisms would be discovered by these traditional collection, isolation and culturing techniques. Naturally we know most about micro-organisms that are medically important, i.e. those that cause disease, allergic reactions or are beneficial to us.
Have a browse through the light blue section of this (UK) link: http://www.biotopics.co.uk/conten.html#ecology
Some pretty scanning (surface) electron microscope images are here: http://www.pbrc.hawaii.edu/bemf/microangela/
When using electron microscopes, sometimes micro-organisms may be discovered when viewing other material, such a plant & animal tissues or cells, e.g. http://www.sunderland.ac.uk/~es0man/tem1.htm
In many cases new micro-organisms are 'discovered' after a new infectious disease is identified or during research into a well known disease or investigating things like soil fertility or the safety of drinking water or the transfer of disease via the air. However scientists are also interested in how the micro-organisms live rather just their appearance as seem under electron microscope. You can use special stains (often heavy metals) under the transmission electron microscope to highlight intra-cellular cell structures.
A 'newly' discovered micro-organism is: http://www.eurekalert.org/pub_releases/1996-09/ORNL-NDBP-170996.php
Regards
Keith ---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
X-from: {distall2-at-yahoo.com} To: {keith.morris-at-ucl.ac.uk} Sent: Thursday, October 06, 2005 12:47 AM
Hello All,
What is a doul layer dvd?
And no, I don't believe that is "dual" misspelled.
-- Karen L. Kelley ICBR Electron Microscopy Manager University of Florida ICBR Electron Microscopy Core Lab Bartram Hall Room 214 Box 118525 Gainesville Florida Lab: 352-392-1184 fax: 352-846-0251 Email: klk-at-biotech.ufl.edu Southeastern Microscopy Society Treasurer http://www.biotech.ufl.edu/EM/
==============================Original Headers============================== 6, 21 -- From klk-at-biotech.ufl.edu Mon Oct 10 15:01:27 2005 6, 21 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9AK1QHk007604 6, 21 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 15:01:26 -0500 6, 21 -- Received: from [128.227.60.184] (empc41719.dhcp.clas.ufl.edu [128.227.60.184]) 6, 21 -- by smtp.ufl.edu (8.13.4/8.13.4/2.5.0) with ESMTP id j9AK1Nww1708044 6, 21 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 16:01:24 -0400 6, 21 -- Message-ID: {434AC892.8030406-at-biotech.ufl.edu} 6, 21 -- Date: Mon, 10 Oct 2005 16:01:22 -0400 6, 21 -- From: Karen Kelley {klk-at-biotech.ufl.edu} 6, 21 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 6, 21 -- X-Accept-Language: en-us, en 6, 21 -- MIME-Version: 1.0 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- Subject: Doul Layer DVD 6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- X-Spam-Status: hits=-1.524, required=5, tests=BAYES_01 6, 21 -- X-UFL-Spam-Status: hits=-1.524, required=5, tests=BAYES_01 6, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 6, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
I bet you a beer, payable at the New Zealand Microscopy conference early in 2007, that it is, in fact, either a typo or a manifestation of illiteracy.
cheers
rtch
Date sent: Mon, 10 Oct 2005 15:03:13 -0500 To: r.sims-at-auckland.ac.nz X-from: klk-at-biotech.ufl.edu Send reply to: klk-at-biotech.ufl.edu
That is a DVD that hold 'too' times the information of a standard dvd.
klk-at-biotech.ufl.edu wrote:
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-- Gerald Bourne Major Analytical Instrumentation Center Department of Materials Science and Engineering University of Florida 107H MAEC P.O. Box 116400 Gainesville, FL 32611 (352) 392-6985 (352) 392-0390 fax
==============================Original Headers============================== 6, 23 -- From grb-at-ufl.edu Mon Oct 10 15:28:00 2005 6, 23 -- Received: from smtp.ufl.edu (sp11en1.nerdc.ufl.edu [128.227.74.11]) 6, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9AKS0gs024957 6, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 15:28:00 -0500 6, 23 -- Received: from [10.227.244.234] ([10.227.244.234]) 6, 23 -- by smtp.ufl.edu (8.13.1/8.13.1/2.5.0) with ESMTP id j9AKRwUt054944 6, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 16:27:59 -0400 6, 23 -- Message-ID: {434ACDDD.7020001-at-ufl.edu} 6, 23 -- Date: Mon, 10 Oct 2005 16:23:57 -0400 6, 23 -- From: Gerald Bourne {grb-at-ufl.edu} 6, 23 -- User-Agent: Mozilla Thunderbird 0.7 (Windows/20040616) 6, 23 -- X-Accept-Language: en-us, en 6, 23 -- MIME-Version: 1.0 6, 23 -- To: Microscopy-at-microscopy.com 6, 23 -- Subject: Re: [Microscopy] Doul Layer DVD 6, 23 -- References: {200510102008.j9AK8SAL015785-at-ns.microscopy.com} 6, 23 -- In-Reply-To: {200510102008.j9AK8SAL015785-at-ns.microscopy.com} 6, 23 -- Content-Type: text/plain; charset=us-ascii; format=flowed 6, 23 -- Content-Transfer-Encoding: 7bit 6, 23 -- X-Spam-Status: hits=-4.9, required=5, tests=BAYES_00 6, 23 -- X-UFL-Spam-Status: hits=-4.9, required=5, tests=BAYES_00 6, 23 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 6, 23 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
Hello, I need to order some oil filters and odor element for two Edwards rotary pumps (RV8). The elements go into a housing known as an EMF10. Does anyone have an inexpensive source for these? I've seen a price of $220 for one oil filter and odor filter. Any advice appreciated.
Thanks
\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\ Gordon Ante Vrdoljak Electron Microscope Lab AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall gvrdolja-at-nature.berkeley.edu UC Berkeley phone (510) 642-2085 Berkeley CA 94720-3330 fax (510) 643-6207 cell (510) 290-6793
==============================Original Headers============================== 3, 24 -- From gvrdolja-at-nature.berkeley.edu Mon Oct 10 15:45:20 2005 3, 24 -- Received: from nature.Berkeley.EDU (nature.Berkeley.EDU [128.32.253.219]) 3, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9AKjKOg001255 3, 24 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 15:45:20 -0500 3, 24 -- Received: from localhost (localhost [127.0.0.1]) 3, 24 -- by nature.Berkeley.EDU (Postfix) with ESMTP id DE82EC1E48 3, 24 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 13:45:18 -0700 (PDT) 3, 24 -- Received: from nature.Berkeley.EDU ([127.0.0.1]) 3, 24 -- by localhost (nature.berkeley.edu [127.0.0.1]) (amavisd-new, port 10024) 3, 24 -- with ESMTP id 15471-05-8 for {microscopy-at-microscopy.com} ; 3, 24 -- Mon, 10 Oct 2005 13:45:10 -0700 (PDT) 3, 24 -- Received: by nature.Berkeley.EDU (Postfix, from userid 7458) 3, 24 -- id 7D8ADC1E67; Mon, 10 Oct 2005 13:45:04 -0700 (PDT) 3, 24 -- Received: from localhost (localhost [127.0.0.1]) 3, 24 -- by nature.Berkeley.EDU (Postfix) with ESMTP id 6098DC1E4F 3, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 13:45:04 -0700 (PDT) 3, 24 -- Date: Mon, 10 Oct 2005 13:45:04 -0700 (PDT) 3, 24 -- From: Gordon Vrololjak {gvrdolja-at-nature.berkeley.edu} 3, 24 -- To: Microscopy-at-microscopy.com 3, 24 -- Subject: oil filters 3, 24 -- Message-ID: {Pine.SOC.4.64.0510101343010.8146-at-nature.Berkeley.EDU} 3, 24 -- MIME-Version: 1.0 3, 24 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 3, 24 -- X-Virus-Scanned: amavisd-new at nature.berkeley.edu ==============================End of - Headers==============================
Actually, I believe it is a misspelling. If it is, it's the latest flavor of optical recording media (but not the last). They actually record on two separate layers (hence dual) on the same side of the disc.
If you dying to know the details, everything you never wanted to know about CDs' and DVD's can be had at
The document itself is an evaluation of the life expectancy of optical media. But it contains a very understandable description of the various types of optical disk.
Bede Willenbring Research Chemist H.B. Fuller Company
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hello All,
What is a doul layer dvd?
And no, I don't believe that is "dual" misspelled.
-- Karen L. Kelley ICBR Electron Microscopy Manager University of Florida ICBR Electron Microscopy Core Lab Bartram Hall Room 214 Box 118525 Gainesville Florida Lab: 352-392-1184 fax: 352-846-0251 Email: klk-at-biotech.ufl.edu Southeastern Microscopy Society Treasurer http://www.biotech.ufl.edu/EM/
==============================Original Headers============================== 6, 21 -- From klk-at-biotech.ufl.edu Mon Oct 10 15:01:27 2005 6, 21 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9AK1QHk007604 6, 21 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 15:01:26 -0500 6, 21 -- Received: from [128.227.60.184] (empc41719.dhcp.clas.ufl.edu [128.227.60.184]) 6, 21 -- by smtp.ufl.edu (8.13.4/8.13.4/2.5.0) with ESMTP id j9AK1Nww1708044 6, 21 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 16:01:24 -0400 6, 21 -- Message-ID: {434AC892.8030406-at-biotech.ufl.edu} 6, 21 -- Date: Mon, 10 Oct 2005 16:01:22 -0400 6, 21 -- From: Karen Kelley {klk-at-biotech.ufl.edu} 6, 21 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 6, 21 -- X-Accept-Language: en-us, en 6, 21 -- MIME-Version: 1.0 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- Subject: Doul Layer DVD 6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- X-Spam-Status: hits=-1.524, required=5, tests=BAYES_01 6, 21 -- X-UFL-Spam-Status: hits=-1.524, required=5, tests=BAYES_01 6, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 6, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
==============================Original Headers============================== 20, 20 -- From Bede.Willenbring-at-hbfuller.com Mon Oct 10 17:12:39 2005 20, 20 -- Received: from uswlksn16.na.hbfuller.com (uswlksn16-o.hbfuller.com [198.57.14.116]) 20, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9AMCd4n010880 20, 20 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 17:12:39 -0500 20, 20 -- Received: from mail.na.hbfuller.com ([172.16.9.35]) 20, 20 -- by uswlksn16.na.hbfuller.com (SAVSMTP 3.1.7.47) with SMTP id M2005101017123528753 20, 20 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 17:12:35 -0500 20, 20 -- Received: from USCITGGD-MTA by mail.na.hbfuller.com 20, 20 -- with Novell_GroupWise; Mon, 10 Oct 2005 17:12:35 -0500 20, 20 -- Message-Id: {s34aa103.022-at-mail.na.hbfuller.com} 20, 20 -- X-Mailer: Novell GroupWise Internet Agent 6.5.4 20, 20 -- Date: Mon, 10 Oct 2005 17:12:17 -0500 20, 20 -- From: "Bede Willenbring" {Bede.Willenbring-at-hbfuller.com} 20, 20 -- To: {microscopy-at-microscopy.com} 20, 20 -- Subject: Re: [Microscopy] Doul Layer DVD 20, 20 -- Mime-Version: 1.0 20, 20 -- Content-Type: text/plain; charset=US-ASCII 20, 20 -- Content-Disposition: inline 20, 20 -- Content-Transfer-Encoding: 8bit 20, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9AMCd4n010880 ==============================End of - Headers==============================
to all who responded to my plaintif plea, thanks. you were all correct. unfortunately, the company no longer carries their line of historical objects. but i did get some interesting information. seems the microscopes were a labour of love by an "old doctor in Chicago" who custom made the replicas. they were full size, working models in steriling silver. the company purchased 4 to see if they would be a seller, but no-one took them up on them. eventually they were sold off. the person who made the instruments is apparently "no longer with us, he's gone on...." in the words of the source.
suppose i should give recognition to Carolina Biological and their 30year employee/microscope expert who passed the information on.
oh, and phil - the other companies were a good thought, but they couldn't help. one did suggest i contact an antique dealer. i don't even want to think what an original would cost. not even if i won your Power Ball lottery that you guys run in some of the states.....
paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-3926
==============================Original Headers============================== 5, 13 -- From zaluzec-at-microscopy.com Mon Oct 10 21:57:29 2005 5, 13 -- Received: from [206.69.208.22] (msdvpn27.msd.anl.gov [130.202.238.91]) 5, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9B2vRf1003038 5, 13 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 21:57:29 -0500 5, 13 -- Mime-Version: 1.0 5, 13 -- X-Sender: (Unverified) 5, 13 -- Message-Id: {p06110402bf70da890421-at-[206.69.208.22]} 5, 13 -- Date: Mon, 10 Oct 2005 21:57:26 -0500 5, 13 -- To: microscopy-at-microscopy.com 5, 13 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca} (by way of 5, 13 -- MicroscopyListserver) 5, 13 -- Subject: thanks re Van Leeuwenhoek lenses and microscopes 5, 13 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
I would like to thank everyone who replied to my questions concerning vacuum and electron gun modification. Regards,
Dr Grzegorz Tylko Department of Cytology and Histology Institute of Zoology Jagiellonian University ul. Ingardena 6 30-060 Krakow fax: +48-12-634-49-51 phone: +48-12-633-63-77 ext. 2425 mobile phone: +48-602-535-041 e-mail: tylko-at-zuk.iz.uj.edu.pl
==============================Original Headers============================== 3, 42 -- From tylko-at-zuk.iz.uj.edu.pl Tue Oct 11 02:14:57 2005 3, 42 -- Received: from theta.uoks.uj.edu.pl (theta.uoks.uj.edu.pl [149.156.89.30]) 3, 42 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9B7ErQa016624 3, 42 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 02:14:57 -0500 3, 42 -- Received: from zuk.iz.uj.edu.pl (zuk.iz.uj.edu.pl [149.156.72.18]) 3, 42 -- by theta.uoks.uj.edu.pl (8.13.1/8.13.1) with ESMTP id j9B7Em5Q015218 3, 42 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 09:14:49 +0200 (CEST) 3, 42 -- Received: from ZUK/SpoolDir by zuk.iz.uj.edu.pl (Mercury 1.48); 3, 42 -- 11 Oct 05 09:14:48 +0100 3, 42 -- Received: from SpoolDir by ZUK (Mercury 1.48); 11 Oct 05 09:14:42 +0100 3, 42 -- Received: from zch0 (149.156.77.98) by zuk.iz.uj.edu.pl (Mercury 1.48); 3, 42 -- 11 Oct 05 09:14:36 +0100 3, 42 -- Message-ID: {004b01c5ce34$1bb0ccc0$624d9c95-at-zch0} 3, 42 -- From: "Grzegorz Tylko" {tylko-at-zuk.iz.uj.edu.pl} 3, 42 -- To: {microscopy-at-microscopy.com} 3, 42 -- Subject: thanks re vacuum pumps and LaB6 3, 42 -- Date: Tue, 11 Oct 2005 09:19:24 +0200 3, 42 -- MIME-Version: 1.0 3, 42 -- Content-Type: text/plain; 3, 42 -- format=flowed; 3, 42 -- charset="iso-8859-2"; 3, 42 -- reply-type=original 3, 42 -- Content-Transfer-Encoding: 7bit 3, 42 -- X-Priority: 3 3, 42 -- X-MSMail-Priority: Normal 3, 42 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 3, 42 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 3, 42 -- X-Spam-Report: Spam detection software, running on the system "pandora", has 3, 42 -- identified this incoming email as possible spam. The original message 3, 42 -- has been attached to this so you can view it (if it isn't spam) or label 3, 42 -- similar future email. If you have any questions, see 3, 42 -- the administrator of that system for details. 3, 42 -- Content preview: I would like to thank everyone who replied to my 3, 42 -- questions concerning vacuum and electron gun modification. Regards, Dr 3, 42 -- Grzegorz Tylko Department of Cytology and Histology Institute of 3, 42 -- Zoology Jagiellonian University ul. Ingardena 6 30-060 Krakow fax: 3, 42 -- +48-12-634-49-51 phone: +48-12-633-63-77 ext. 2425 mobile phone: 3, 42 -- +48-602-535-041 e-mail: tylko-at-zuk.iz.uj.edu.pl [...] 3, 42 -- Content analysis details: (-2.8 points, 5.0 required) 3, 42 -- pts rule name description 3, 42 -- ---- ---------------------- -------------------------------------------------- 3, 42 -- -2.8 ALL_TRUSTED Did not pass through any untrusted hosts ==============================End of - Headers==============================
Can I join in the fun? Bon Jovi are releasing a Dual disc soon. Apparently it is a CD one side and a DVD on the other. Yippee - I would also like my computer to play 7" singles.
Dave
-----Original Message----- X-from: Bede.Willenbring-at-hbfuller.com [mailto:Bede.Willenbring-at-hbfuller.com] Sent: 10 October 2005 23:20 To: David Patton
Actually, I believe it is a misspelling. If it is, it's the latest flavor of optical recording media (but not the last). They actually record on two separate layers (hence dual) on the same side of the disc.
If you dying to know the details, everything you never wanted to know about CDs' and DVD's can be had at
The document itself is an evaluation of the life expectancy of optical media. But it contains a very understandable description of the various types of optical disk.
Bede Willenbring Research Chemist H.B. Fuller Company
------------------------------------------------------------------------ ---- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hello All,
What is a doul layer dvd?
And no, I don't believe that is "dual" misspelled.
-- Karen L. Kelley ICBR Electron Microscopy Manager University of Florida ICBR Electron Microscopy Core Lab Bartram Hall Room 214 Box 118525 Gainesville Florida Lab: 352-392-1184 fax: 352-846-0251 Email: klk-at-biotech.ufl.edu Southeastern Microscopy Society Treasurer http://www.biotech.ufl.edu/EM/
==============================Original Headers============================== 6, 21 -- From klk-at-biotech.ufl.edu Mon Oct 10 15:01:27 2005 6, 21 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9AK1QHk007604 6, 21 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 15:01:26 -0500 6, 21 -- Received: from [128.227.60.184] (empc41719.dhcp.clas.ufl.edu [128.227.60.184]) 6, 21 -- by smtp.ufl.edu (8.13.4/8.13.4/2.5.0) with ESMTP id j9AK1Nww1708044 6, 21 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 16:01:24 -0400 6, 21 -- Message-ID: {434AC892.8030406-at-biotech.ufl.edu} 6, 21 -- Date: Mon, 10 Oct 2005 16:01:22 -0400 6, 21 -- From: Karen Kelley {klk-at-biotech.ufl.edu} 6, 21 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 6, 21 -- X-Accept-Language: en-us, en 6, 21 -- MIME-Version: 1.0 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- Subject: Doul Layer DVD 6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- X-Spam-Status: hits=-1.524, required=5, tests=BAYES_01 6, 21 -- X-UFL-Spam-Status: hits=-1.524, required=5, tests=BAYES_01 6, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 6, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
==============================Original Headers============================== 20, 20 -- From Bede.Willenbring-at-hbfuller.com Mon Oct 10 17:12:39 2005 20, 20 -- Received: from uswlksn16.na.hbfuller.com (uswlksn16-o.hbfuller.com [198.57.14.116]) 20, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9AMCd4n010880 20, 20 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 17:12:39 -0500 20, 20 -- Received: from mail.na.hbfuller.com ([172.16.9.35]) 20, 20 -- by uswlksn16.na.hbfuller.com (SAVSMTP 3.1.7.47) with SMTP id M2005101017123528753 20, 20 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 17:12:35 -0500 20, 20 -- Received: from USCITGGD-MTA by mail.na.hbfuller.com 20, 20 -- with Novell_GroupWise; Mon, 10 Oct 2005 17:12:35 -0500 20, 20 -- Message-Id: {s34aa103.022-at-mail.na.hbfuller.com} 20, 20 -- X-Mailer: Novell GroupWise Internet Agent 6.5.4 20, 20 -- Date: Mon, 10 Oct 2005 17:12:17 -0500 20, 20 -- From: "Bede Willenbring" {Bede.Willenbring-at-hbfuller.com} 20, 20 -- To: {microscopy-at-microscopy.com} 20, 20 -- Subject: Re: [Microscopy] Doul Layer DVD 20, 20 -- Mime-Version: 1.0 20, 20 -- Content-Type: text/plain; charset=US-ASCII 20, 20 -- Content-Disposition: inline 20, 20 -- Content-Transfer-Encoding: 8bit 20, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9AMCd4n010880 ==============================End of - Headers==============================
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==============================Original Headers============================== 32, 32 -- From David.Patton-at-uwe.ac.uk Tue Oct 11 02:48:32 2005 32, 32 -- Received: from mailapp01.uwe.ac.uk (mailapp01.uwe.ac.uk [164.11.132.61]) 32, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9B7mVvv025522 32, 32 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 02:48:31 -0500 32, 32 -- Received: from (164.11.132.62) by mailapp01.uwe.ac.uk via smtp 32, 32 -- id 6a56_7c11fe5a_3a2b_11da_9f0c_0002b3c946e4; 32, 32 -- Tue, 11 Oct 2005 08:49:00 +0100 32, 32 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 32, 32 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121]) 32, 32 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 32, 32 -- 2005)) with ESMTP id {0IO600H71R0TJL-at-mta02.uwe.ac.uk} for 32, 32 -- microscopy-at-microscopy.com; Tue, 11 Oct 2005 08:48:29 +0100 (BST) 32, 32 -- Date: Tue, 11 Oct 2005 08:48:30 +0100 32, 32 -- From: David Patton {David.Patton-at-uwe.ac.uk} 32, 32 -- Subject: RE: [Microscopy] Re: Doul Layer DVD 32, 32 -- To: microscopy-at-microscopy.com 32, 32 -- Message-id: {F247F674896BE243AD8263C5280E2BDBF84DBF-at-NBUEGEN01} 32, 32 -- MIME-version: 1.0 32, 32 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5.6944.0 32, 32 -- Content-type: text/plain; charset=us-ascii 32, 32 -- Content-class: urn:content-classes:message 32, 32 -- Thread-topic: [Microscopy] Re: Doul Layer DVD 32, 32 -- Thread-index: AcXN6LWXMEkZ2qc/TqKetsbLl72WAgATzFQw 32, 32 -- X-MS-Has-Attach: 32, 32 -- X-MS-TNEF-Correlator: 32, 32 -- X-NAI-Spam-Score: -2.5 32, 32 -- X-NAI-Spam-Rules: 1 Rules triggered 32, 32 -- BAYES_00=-2.5 32, 32 -- X-NAIMIME-Disclaimer: 1 32, 32 -- X-NAIMIME-Modified: 1 32, 32 -- Content-Transfer-Encoding: 8bit 32, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9B7mVvv025522 ==============================End of - Headers==============================
http://www.dvd12.info//dvd-13.html scroll down, left link-bar-area, Press (Control&letterF) (FIND-Function ,search for } doul {) you will find the term (& LINK bar) "Doul Layer DVD" but no further explanation exept "Double Layer DVD"
Also on http://nessmp3.com/forums/index.php?showtopic=579 with the } find function { (Ctrl&letter F) and searching for } doul {:
you will find:
..........} Doul Layer DVD's { right now in my opinion are just a waste of money. You need a player that supports the format and they cost big $$$. I'de wait a few years before that investment. I know most burners support it right now but do you want to shorten the life of your burner to watch a movie anyway? {
Seems to be an insiders' "short cut" for Double layer DVD ?...Thanks for the question, I haven't heared anything about such new recording medium until now...interesting....
regards, Wolfgang Muss SALK (Salzburger Landeskliniken GesmbH) Paracelsus Medical Private University (PMU) Institute of Pathology Electron Microscopy Lab Muellner Hauptstrasse 48 A-5020 SALZBURG, Austria/Europe Phone work: +43+662+4482+4720 Mobile phone work:+43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss") E-Mail work: W.Muss-at-SALK.at Mobile-phone private: ++43+676+5 369-456 E-Mail private: wij.Muss-at-aon.at
---------- Von: David.Patton-at-uwe.ac.uk[SMTP:David.Patton-at-uwe.ac.uk] Antwort an: David.Patton-at-uwe.ac.uk Gesendet: Dienstag, 11. Oktober 2005 09:52 An: W.Muss-at-salk.at Betreff: [Microscopy] Doul Layer DVD
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Can I join in the fun? Bon Jovi are releasing a Dual disc soon. Apparently it is a CD one side and a DVD on the other. Yippee - I would also like my computer to play 7" singles.
Dave
-----Original Message----- X-from: Bede.Willenbring-at-hbfuller.com [mailto:Bede.Willenbring-at-hbfuller.com] Sent: 10 October 2005 23:20 To: David Patton
Actually, I believe it is a misspelling. If it is, it's the latest flavor of optical recording media (but not the last). They actually record on two separate layers (hence dual) on the same side of the disc.
If you dying to know the details, everything you never wanted to know about CDs' and DVD's can be had at
The document itself is an evaluation of the life expectancy of optical media. But it contains a very understandable description of the various types of optical disk.
Bede Willenbring Research Chemist H.B. Fuller Company
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Hello All,
What is a doul layer dvd?
And no, I don't believe that is "dual" misspelled.
-- Karen L. Kelley ICBR Electron Microscopy Manager University of Florida ICBR Electron Microscopy Core Lab Bartram Hall Room 214 Box 118525 Gainesville Florida Lab: 352-392-1184 fax: 352-846-0251 Email: klk-at-biotech.ufl.edu Southeastern Microscopy Society Treasurer http://www.biotech.ufl.edu/EM/
==============================Original Headers============================== 6, 21 -- From klk-at-biotech.ufl.edu Mon Oct 10 15:01:27 2005 6, 21 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9AK1QHk007604 6, 21 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 15:01:26 -0500 6, 21 -- Received: from [128.227.60.184] (empc41719.dhcp.clas.ufl.edu [128.227.60.184]) 6, 21 -- by smtp.ufl.edu (8.13.4/8.13.4/2.5.0) with ESMTP id j9AK1Nww1708044 6, 21 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 16:01:24 -0400 6, 21 -- Message-ID: {434AC892.8030406-at-biotech.ufl.edu} 6, 21 -- Date: Mon, 10 Oct 2005 16:01:22 -0400 6, 21 -- From: Karen Kelley {klk-at-biotech.ufl.edu} 6, 21 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 6, 21 -- X-Accept-Language: en-us, en 6, 21 -- MIME-Version: 1.0 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- Subject: Doul Layer DVD 6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- X-Spam-Status: hits=-1.524, required=5, tests=BAYES_01 6, 21 -- X-UFL-Spam-Status: hits=-1.524, required=5, tests=BAYES_01 6, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 6, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
==============================Original Headers============================== 20, 20 -- From Bede.Willenbring-at-hbfuller.com Mon Oct 10 17:12:39 2005 20, 20 -- Received: from uswlksn16.na.hbfuller.com (uswlksn16-o.hbfuller.com [198.57.14.116]) 20, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9AMCd4n010880 20, 20 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 17:12:39 -0500 20, 20 -- Received: from mail.na.hbfuller.com ([172.16.9.35]) 20, 20 -- by uswlksn16.na.hbfuller.com (SAVSMTP 3.1.7.47) with SMTP id M2005101017123528753 20, 20 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2005 17:12:35 -0500 20, 20 -- Received: from USCITGGD-MTA by mail.na.hbfuller.com 20, 20 -- with Novell_GroupWise; Mon, 10 Oct 2005 17:12:35 -0500 20, 20 -- Message-Id: {s34aa103.022-at-mail.na.hbfuller.com} 20, 20 -- X-Mailer: Novell GroupWise Internet Agent 6.5.4 20, 20 -- Date: Mon, 10 Oct 2005 17:12:17 -0500 20, 20 -- From: "Bede Willenbring" {Bede.Willenbring-at-hbfuller.com} 20, 20 -- To: {microscopy-at-microscopy.com} 20, 20 -- Subject: Re: [Microscopy] Doul Layer DVD 20, 20 -- Mime-Version: 1.0 20, 20 -- Content-Type: text/plain; charset=US-ASCII 20, 20 -- Content-Disposition: inline 20, 20 -- Content-Transfer-Encoding: 8bit 20, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9AMCd4n010880 ==============================End of - Headers==============================
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==============================Original Headers============================== 32, 32 -- From David.Patton-at-uwe.ac.uk Tue Oct 11 02:48:32 2005 32, 32 -- Received: from mailapp01.uwe.ac.uk (mailapp01.uwe.ac.uk [164.11.132.61]) 32, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9B7mVvv025522 32, 32 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 02:48:31 -0500 32, 32 -- Received: from (164.11.132.62) by mailapp01.uwe.ac.uk via smtp 32, 32 -- id 6a56_7c11fe5a_3a2b_11da_9f0c_0002b3c946e4; 32, 32 -- Tue, 11 Oct 2005 08:49:00 +0100 32, 32 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 32, 32 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121]) 32, 32 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 32, 32 -- 2005)) with ESMTP id {0IO600H71R0TJL-at-mta02.uwe.ac.uk} for 32, 32 -- microscopy-at-microscopy.com; Tue, 11 Oct 2005 08:48:29 +0100 (BST) 32, 32 -- Date: Tue, 11 Oct 2005 08:48:30 +0100 32, 32 -- From: David Patton {David.Patton-at-uwe.ac.uk} 32, 32 -- Subject: RE: [Microscopy] Re: Doul Layer DVD 32, 32 -- To: microscopy-at-microscopy.com 32, 32 -- Message-id: {F247F674896BE243AD8263C5280E2BDBF84DBF-at-NBUEGEN01} 32, 32 -- MIME-version: 1.0 32, 32 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5.6944.0 32, 32 -- Content-type: text/plain; charset=us-ascii 32, 32 -- Content-class: urn:content-classes:message 32, 32 -- Thread-topic: [Microscopy] Re: Doul Layer DVD 32, 32 -- Thread-index: AcXN6LWXMEkZ2qc/TqKetsbLl72WAgATzFQw 32, 32 -- X-MS-Has-Attach: 32, 32 -- X-MS-TNEF-Correlator: 32, 32 -- X-NAI-Spam-Score: -2.5 32, 32 -- X-NAI-Spam-Rules: 1 Rules triggered 32, 32 -- BAYES_00=-2.5 32, 32 -- X-NAIMIME-Disclaimer: 1 32, 32 -- X-NAIMIME-Modified: 1 32, 32 -- Content-Transfer-Encoding: 8bit 32, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9B7mVvv025522 ==============================End of - Headers==============================
==============================Original Headers============================== 53, 28 -- From W.Muss-at-salk.at Tue Oct 11 03:16:28 2005 53, 28 -- Received: from n1fm211.lks.local (hermes.lks.at [193.170.167.9]) 53, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9B8GRTZ010486 53, 28 -- for {Microscopy-at-Microscopy.Com} ; Tue, 11 Oct 2005 03:16:28 -0500 53, 28 -- Received: from localhost (localhost [127.0.0.1]) 53, 28 -- by hermes.lks.at (Postfix) with ESMTP id DA3735A9031; 53, 28 -- Tue, 11 Oct 2005 10:16:18 +0200 (CEST) 53, 28 -- Received: from hermes.lks.at ([127.0.0.1]) 53, 28 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024) 53, 28 -- with ESMTP id 15117-06; Tue, 11 Oct 2005 10:16:17 +0200 (CEST) 53, 28 -- Received: from c1pa003 (unknown [192.168.42.3]) 53, 28 -- by hermes.lks.at (Postfix) with SMTP id 384C25A9036; 53, 28 -- Tue, 11 Oct 2005 10:16:17 +0200 (CEST) 53, 28 -- Received: by localhost with Microsoft MAPI; Tue, 11 Oct 2005 10:16:16 +0200 53, 28 -- Message-ID: {01C5CE4C.D0C78460.W.Muss-at-salk.at} 53, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at} 53, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at} 53, 28 -- To: "'klk-at-biotech.ufl.edu'" {klk-at-biotech.ufl.edu} 53, 28 -- Cc: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-Microscopy.Com} 53, 28 -- Subject: [Microscopy] Re: Doul Layer DVD 53, 28 -- Date: Tue, 11 Oct 2005 10:16:15 +0200 53, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at} 53, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor 53, 28 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211 53, 28 -- MIME-Version: 1.0 53, 28 -- Content-Type: text/plain; charset="us-ascii" 53, 28 -- Content-Transfer-Encoding: 7bit 53, 28 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at ==============================End of - Headers==============================
} to all who responded to my plaintif plea, thanks. you were all correct. unfortunately, the company no longer carries their line of historical objects. but i did get some interesting information. seems the microscopes were a labour of love by an "old doctor in Chicago" who custom made the replicas. they were full size, working models in steriling silver. the company purchased 4 to see if they would be a seller, but no-one took them up on them. eventually they were sold off. the person who made the instruments is apparently "no longer with us, he's gone on...." in the words of the source. } } suppose i should give recognition to Carolina Biological and their 30year employee/microscope expert who passed the information on. } } oh, and phil - the other companies were a good thought, but they couldn't help. one did suggest i contact an antique dealer. i don't even want to think what an original would cost. not even if i won your Power Ball lottery that you guys run in some of the states..... } Hi Paul,
Commissioning some one to build a few might not be too expensive if you can find the right person. To be authentic they are have to almost be made one off any way. Brass or copper would be more authentic than silver and take on a more pleasing patina with age. If you hurry copper will cost less than silver:{
Gordon Gordon Couger
I collect links on information related to light microscopes. www.couger.com/microscope/links/gclinks.html Please forward anything you think might be useful to others. Microscope Documentation is at www.science-info.org
==============================Original Headers============================== 8, 20 -- From gcc-at-couger.com Tue Oct 11 03:33:49 2005 8, 20 -- Received: from centrmmtao02.cox.net (centrmmtao02.cox.net [70.168.83.82]) 8, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9B8XnEY019189 8, 20 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 03:33:49 -0500 8, 20 -- Received: from [127.0.0.1] (really [68.12.242.13]) by centrmmtao02.cox.net 8, 20 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 8, 20 -- id {20051011083346.FOUX21553.centrmmtao02.cox.net-at-[127.0.0.1]} ; 8, 20 -- Tue, 11 Oct 2005 04:33:46 -0400 8, 20 -- Message-ID: {434B7906.4000301-at-couger.com} 8, 20 -- Date: Tue, 11 Oct 2005 03:34:14 -0500 8, 20 -- From: Gordon Couger {gcc-at-couger.com} 8, 20 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 8, 20 -- X-Accept-Language: en-us, en 8, 20 -- MIME-Version: 1.0 8, 20 -- To: paul_hazelton-at-umanitoba.ca, microscopy-at-microscopy.com 8, 20 -- Subject: Re: [Microscopy] thanks re Van Leeuwenhoek lenses and microscopes 8, 20 -- References: {200510110303.j9B33B5X008719-at-ns.microscopy.com} 8, 20 -- In-Reply-To: {200510110303.j9B33B5X008719-at-ns.microscopy.com} 8, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
X-from a quick search of the web doul layer is obviously a misspelling of dual layer (e.g. Chitty Chitty Bang Bang on doul layer disk - I don't think so). Dual layer where the laser can actually write to two dye layers on one side of the disk. This doubles the capacity of the disk from 4.7Gb to 9.4Gb and is the reason DVD film copying requires a great deal of time to compress the 9.4Gb into 4.7Gb of a -R or +R standard single layer DVD - up to 8 hours (whereas decrypting the copyright protection takes a few minutes).
With regard to backing up important scientific data, you should be aware that archived CD-Rs should be 're-backuped' up to new media every three to five years. Unbranded CD's that have little quality control should be avoided or re-backed up within 6 months of writing. Use datasafe pens (if anything) for writing on the label surface (solvent based 'sharpies' aren't recommended at all, especially the manufacturer), as the CD label surface is actually where the read/write dye is located. Store crucial archived CDs or DVD's in solid tough polythene cases, although paper CD sleeves are fine if handled carefully. Avoid all-polythene clear flexible sleeves of the type often sent out with free CD's as these definitely do gradually damage the CD surface by rubbing.
Of the DVD's only DVD RAM is safe for storage up to 100 years and 300,000 re-writes (especially in its protective caddy) - although the format is difficult to source as a drive these days in its caddy version and the lack of a recovery drive may become a problem in the future. The 'drag&drop' 4.7Gb DVD-RAM can be removed from the caddy or purchased as plain non-caddy disks. LG make excellent non caddy DVD-RAM/-R/+R multiwriters. DVD RAM, being read/write erasable, are very slow to write to though.
DVD dual layer, -R & +Rs are great for film and TV archives where a scratch may just result in the odd dropped frame and at worst a trip to the mall to buy another copy. However that could be your precious image or data file. DVD -R and +Rs scratch very easily, even when stacking or dropping onto carpet, so although they are certainly far better than nothing, all computers in our imaging suite use DVD RAM cartridge drives as well as DVD-R and CD for crucial data. If possible keep another copy of crucial data on a PC hard drive as well (they are so cheap per Gb these days and relatively reliable). Internal hard drives are less likely to suffer from shock (dropping them or things onto them), which is particularly a problem when active with the head unparked (causing the HD head to crash onto the disk surface when read/writing). Secondary internal hard drives are very easy to fit - but keep to one manufacturer. Personally I would avoid dual layer DVD's at the moment as they are really for hi-definition film, are expensive per Gb, and their complexity may lead to data loss in the future (early CD's have already failed due to the printing ink of the label gradually etching into the dye layer).
Always backup with proper backup software such as Nero backItUp where the data is verified as an exact copy of the original file. I never compress files, and large zipped directories frequently corrupt. Personally I always avoid any type of tape backup system, as whenever I have tried to recover lost data the tape archive has been 'corrupted' and I have had to recover data from a standard floppy disk or CD. I keep backup DVD's of my home data at work and visa-versa.
Regards
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
Tel: 020 7608 4050 Fax: 020 7608 4034 email: keith.morris-at-ucl.ac.uk ----- Original Message ----- X-from: {Bede.Willenbring-at-hbfuller.com} To: {keith.morris-at-ucl.ac.uk} Sent: Monday, October 10, 2005 11:19 PM
I like to add to add the following link for those who are interested in food and other micro-organisms under electron microscopes. http://www.magma.ca/~scimat/
Ann Fook Yang EM Unit/ Unite EM AAFC/AAC 960 Carling Ave, Ottawa,Ontario Canada K1A 0C6 yanga-at-agr.gc.ca Telephone/Téléphone: 613-759-1638 Facsimile/Télécopieur: 613-759-1701
Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada
-----Original Message----- X-from: keith.morris-at-ucl.ac.uk [mailto:keith.morris-at-ucl.ac.uk] Sent: Monday, October 10, 2005 6:35 AM To: Yang, Ann-Fook
Dear Masood,
Generally micro-organisms aren't discovered using the electron microsope. They are normally taken from their natural habitat, such as soil, the air, blood and so forth, and may be grown ('cultured') in special media that contains the nutrients they need to survive. The media may be an Agar plate (a jelly or jello like material ) or a soupy broth liquid. The single celled organisms divide in this producing millions of cells (and hopefully all of the type you want). Viruses are more complicated as they require a cell to replicate in. Once there are plenty of micro-organisms in the culture they can be harvested and treated for viewing under the light or electron microscope. Light microscopy is, with the use of special cellular stains, is still very important for micro-organism identification. Once isolated and cultured we can also study them to see how they live and how to kill them if they are dangerous.
So normally the micro-organisms would be discovered by these traditional collection, isolation and culturing techniques. Naturally we know most about micro-organisms that are medically important, i.e. those that cause disease, allergic reactions or are beneficial to us.
Have a browse through the light blue section of this (UK) link: http://www.biotopics.co.uk/conten.html#ecology
Some pretty scanning (surface) electron microscope images are here: http://www.pbrc.hawaii.edu/bemf/microangela/
When using electron microscopes, sometimes micro-organisms may be discovered when viewing other material, such a plant & animal tissues or cells, e.g. http://www.sunderland.ac.uk/~es0man/tem1.htm
In many cases new micro-organisms are 'discovered' after a new infectious disease is identified or during research into a well known disease or investigating things like soil fertility or the safety of drinking water or the transfer of disease via the air. However scientists are also interested in how the micro-organisms live rather just their appearance as seem under electron microscope. You can use special stains (often heavy metals) under the transmission electron microscope to highlight intra-cellular cell structures.
A 'newly' discovered micro-organism is: http://www.eurekalert.org/pub_releases/1996-09/ORNL-NDBP-170996.php
Regards
Keith ---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
X-from: {distall2-at-yahoo.com} To: {keith.morris-at-ucl.ac.uk} Sent: Thursday, October 06, 2005 12:47 AM
Paul, Alan Shinn used to make replicas and sell them, I think for $ 100, but I think he no longer does. If you can't find some place to buy one, or can't convince your coworkers to buy you one, you could always make one yourself, as you'll need something to do in retirement ;o) You can see his plans here:
I'm putting the finishing touches on my own Leeuwenhoek replica, so keep in touch and maybe I'll be able to help you out.
Paul Grover
------------------------------------------------------------------------ May your trails be crooked, winding, lonesome, dangerous, leading to the most amazing view.
- Edward Abbey
==============================Original Headers============================== 13, 24 -- From pgrover-at-bilbo.bio.purdue.edu Tue Oct 11 09:14:41 2005 13, 24 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 13, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9BEEfhV017467 13, 24 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 09:14:41 -0500 13, 24 -- Received: from paklabpgrover (dhcp155-92.bio.purdue.edu [128.210.155.92]) 13, 24 -- by mailhub129.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id j9BEEet6003743 13, 24 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 09:14:40 -0500 13, 24 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu} 13, 24 -- To: {microscopy-at-microscopy.com} 13, 24 -- Subject: thanks re Van Leeuwenhoek microscope 13, 24 -- Date: Tue, 11 Oct 2005 09:14:40 -0500 13, 24 -- Message-ID: {000001c5ce6e$1ec9c030$5c9bd280-at-paklabpgrover} 13, 24 -- MIME-Version: 1.0 13, 24 -- Content-Type: text/plain; 13, 24 -- charset="us-ascii" 13, 24 -- X-Priority: 3 (Normal) 13, 24 -- X-MSMail-Priority: Normal 13, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.4510 13, 24 -- Importance: Normal 13, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 13, 24 -- X-PMX-Version: 4.7.1.128075 13, 24 -- X-PerlMx-Virus-Scanned: Yes 13, 24 -- Content-Transfer-Encoding: 8bit 13, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9BEEfhV017467 ==============================End of - Headers==============================
I appreciate Wolfgang's digging to see if it might be something real. However, checking out those pages and doing my own search for "doul DVD" and "dual DVD", I suspect it is just a spelling error. There were several other spelling errors on those pages which drops their credibility as an authoritative source. Maybe doul will catch on as a tricky abbreviation for double that plays on the word dual. For now, I write it off as a mispelling.
Warren
At 03:17 AM 10/11/05, you wrote:
} Hello and good morning all, } } Just only } supplementary {, what I found: } } } http://www.dvd12.info//dvd-13.html } scroll down, left link-bar-area, Press (Control&letterF) (FIND-Function } ,search for } doul {) you will find the term (& LINK bar) "Doul Layer DVD" } but no further explanation exept "Double Layer DVD" } } Also on } http://nessmp3.com/forums/index.php?showtopic=579 } with the } find function { (Ctrl&letter F) and searching for } doul {: } } you will find: } } ..........} Doul Layer DVD's { right now in my opinion are just a waste of } money. You need a player that supports the format and they cost big $$$. } I'de wait a few years before that investment. I know most burners support } it right now but do you want to shorten the life of your burner to watch a } movie anyway? { } } Seems to be an insiders' "short cut" for Double layer DVD ?...Thanks for } the question, I haven't heared anything about such new recording medium } until now...interesting.... } } regards, } Wolfgang Muss } SALK (Salzburger Landeskliniken GesmbH) } Paracelsus Medical Private University (PMU) } Institute of Pathology } Electron Microscopy Lab } Muellner Hauptstrasse 48 } A-5020 SALZBURG, Austria/Europe
------------------------------------------- No files should be attached to this message ------------------------------------------- Warren E. Straszheim, Ph.D. Materials Analysis and Research Lab Iowa State University 46 Town Engineering Ames IA, 50011-3232
I would like to measure height differences between microvilli on cultured cells that have been imaged by SEM. Is there a program available that does this type of measurement? Would Image J work? Is there a direct correlation between brightness of structure and height of surface structure that could be used in this manner to compare height of microvilli on adjacent cells? Any opinions would be welcomed.
Thanks,
Ann Tisdale Research Associate Schepens eye Research Institute Boston, MA 02114 email: tisdale-at-vision.eri.harvard.edu
==============================Original Headers============================== 4, 20 -- From tisdale-at-vision.eri.harvard.edu Tue Oct 11 10:31:38 2005 4, 20 -- Received: from vision.eri.harvard.edu (vision.eri.harvard.edu [128.103.8.12]) 4, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9BFVad7003038 4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 10:31:37 -0500 4, 20 -- Received: from vision (root-at-localhost) 4, 20 -- by vision.eri.harvard.edu (8.12.11/8.12.11) with SMTP id j9BFVXFc028381 4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 11:31:33 -0400 4, 20 -- Received: from [192.168.3.98] (e03-098.eri.harvard.edu [192.168.3.98]) 4, 20 -- by vision.eri.harvard.edu (8.12.11/8.12.11) with ESMTP id j9BFVWxY028367 4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 11:31:32 -0400 4, 20 -- Mime-Version: 1.0 4, 20 -- Message-Id: {a06110400bf71855420de-at-[192.168.3.98]} 4, 20 -- Date: Tue, 11 Oct 2005 11:26:10 -0400 4, 20 -- To: microscopy-at-microscopy.com 4, 20 -- From: Ann Tisdale {tisdale-at-vision.eri.harvard.edu} 4, 20 -- Subject: SEM height measurements 4, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 20 -- X-SpamTest-Version: SMTP-Filter Version 2.0.0 [0122], KAS/Release 4, 20 -- X-Spamtest-Info: Pass through 4, 20 -- X-Anti-Virus: Kaspersky Anti-Virus for MailServers 5.5.2/RELEASE, bases: 11102005 #144202, status: clean ==============================End of - Headers==============================
Local topography is only one factor that influences the signal you get from a sample. Others are variations in scattering coefficients (mainly the atomic number of the sample), edges, orientation to detector, etc. In other words, there is no direct correlation between brightness and structure, unless you have a very simple case. I doubt that you can get reliable topographic information from just a single image. I think, your best try would be to model a structure and compare the results of a simulation with real measurements. You can google "Monte Carlo Electron" and find references to electron scattering simulations. David Joy has been working in this field for some time.
If you can take stereo images (two images at different angles) of your sample, you might be able to get some height information, but it appears that the height differences are very small, so that might not be a possibility either.
The only other technique that I can think of right now would be an AFM type of measurement. Have you looked into that?
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: tisdale-at-vision.eri.harvard.edu [mailto:tisdale-at-vision.eri.harvard.edu] Sent: Tuesday, October 11, 2005 9:39 AM To: Mike Bode
I would like to measure height differences between microvilli on cultured cells that have been imaged by SEM. Is there a program available that does this type of measurement? Would Image J work? Is there a direct correlation between brightness of structure and height of surface structure that could be used in this manner to compare height of microvilli on adjacent cells? Any opinions would be welcomed.
Thanks,
Ann Tisdale Research Associate Schepens eye Research Institute Boston, MA 02114 email: tisdale-at-vision.eri.harvard.edu
==============================Original Headers============================== 4, 20 -- From tisdale-at-vision.eri.harvard.edu Tue Oct 11 10:31:38 2005 4, 20 -- Received: from vision.eri.harvard.edu (vision.eri.harvard.edu [128.103.8.12]) 4, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9BFVad7003038 4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 10:31:37 -0500 4, 20 -- Received: from vision (root-at-localhost) 4, 20 -- by vision.eri.harvard.edu (8.12.11/8.12.11) with SMTP id j9BFVXFc028381 4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 11:31:33 -0400 4, 20 -- Received: from [192.168.3.98] (e03-098.eri.harvard.edu [192.168.3.98]) 4, 20 -- by vision.eri.harvard.edu (8.12.11/8.12.11) with ESMTP id j9BFVWxY028367 4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 11:31:32 -0400 4, 20 -- Mime-Version: 1.0 4, 20 -- Message-Id: {a06110400bf71855420de-at-[192.168.3.98]} 4, 20 -- Date: Tue, 11 Oct 2005 11:26:10 -0400 4, 20 -- To: microscopy-at-microscopy.com 4, 20 -- From: Ann Tisdale {tisdale-at-vision.eri.harvard.edu} 4, 20 -- Subject: SEM height measurements 4, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 20 -- X-SpamTest-Version: SMTP-Filter Version 2.0.0 [0122], KAS/Release 4, 20 -- X-Spamtest-Info: Pass through 4, 20 -- X-Anti-Virus: Kaspersky Anti-Virus for MailServers 5.5.2/RELEASE, bases: 11102005 #144202, status: clean ==============================End of - Headers==============================
==============================Original Headers============================== 18, 24 -- From Mike.Bode-at-soft-imaging.net Tue Oct 11 11:50:05 2005 18, 24 -- Received: from mail.soft-imaging.de (mail.soft-imaging.de [62.180.61.131]) 18, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9BGo5Gs012730 18, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 11:50:05 -0500 18, 24 -- Received: from muenster.soft-imaging.net (muenster.soft-imaging.de [192.168.118.231]) 18, 24 -- by mail.soft-imaging.de (8.11.6/8.11.6/SuSE Linux 0.5) with ESMTP id j9BGo4H31009; 18, 24 -- Tue, 11 Oct 2005 18:50:04 +0200 18, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 18, 24 -- Content-class: urn:content-classes:message 18, 24 -- MIME-Version: 1.0 18, 24 -- Content-Type: text/plain; 18, 24 -- charset="iso-8859-1" 18, 24 -- Subject: RE: [Microscopy] SEM height measurements 18, 24 -- Date: Tue, 11 Oct 2005 18:44:34 +0200 18, 24 -- Message-ID: {6D0150089E1EA046BD96C68C50608C2301F277E1-at-ms-s-gws.soft-imaging.net} 18, 24 -- X-MS-Has-Attach: 18, 24 -- X-MS-TNEF-Correlator: 18, 24 -- Thread-Topic: [Microscopy] SEM height measurements 18, 24 -- Thread-Index: AcXOeeOSqQpUvgnwQCypBBw0pe0BjQAB7MyA 18, 24 -- From: "Mike Bode" {Mike.Bode-at-soft-imaging.net} 18, 24 -- To: {Microscopy-at-microscopy.com} 18, 24 -- Cc: {tisdale-at-vision.eri.harvard.edu} 18, 24 -- Content-Transfer-Encoding: 8bit 18, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9BGo5Gs012730 ==============================End of - Headers==============================
as far as I understand the DVDs may be dual layer but I think their capacity isn't twice 4.7Gb but nearer to 8.4Gb. At typically 5x the price (or more) of a 4.7Gb DVD this makes them appear even more uneconomical for data storage. I assume the problem is that they are more likely to be used to 'rip' a video DVD and this may be reflected in the price - but I don't know.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences University of Sunderland Tyne & Wear SR1 3SD UK
e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: keith.morris-at-ucl.ac.uk
Hi all
A request from one of our Centre's users. Can anybody help out.
They are looking for the following article from The Microscope Journal ( - an international journal dedicated to the advancement of all forms of microscopy for the biologist, mineralogist, metallographer or chemist). Published Quarterly by McCrone Research Institute, Edited by Dr. Gary Laughlin.
They are after the following article: Gorski, A., and McCrone, W.C. “Birefringence of Fibers,” THE MICROSCOPE, Vol.46:1, 1998, pp. 3-16
Very happy to pay for photocopying, postage....
Allan Mitchell Otago Centre for Electron Microscopy Department of Anatomy and Structural Biology School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254
EM Centre: http://ocem.otago.ac.nz/ Confocal Centre: http://occm.otago.ac.nz/ Department: http://anatomy.otago.ac.nz/
==============================Original Headers============================== 14, 20 -- From allan.mitchell-at-stonebow.otago.ac.nz Tue Oct 11 22:49:15 2005 14, 20 -- Received: from mailhub1.otago.ac.nz (mailhub1.otago.ac.nz [139.80.64.218]) 14, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9C3nDmG005664 14, 20 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 22:49:14 -0500 14, 20 -- Received: from galadriel.otago.ac.nz (galadriel.otago.ac.nz [139.80.64.213]) 14, 20 -- by mailhub1.otago.ac.nz (8.12.11/8.12.11) with ESMTP id j9C3nAuD004082 14, 20 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2005 16:49:10 +1300 14, 20 -- Received: from [139.80.40.154] (ou040154.otago.ac.nz [139.80.40.154]) 14, 20 -- by galadriel.otago.ac.nz (8.12.8/8.12.8) with ESMTP id j9C3nAdt007713 14, 20 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2005 16:49:10 +1300 (NZDT) 14, 20 -- Mime-Version: 1.0 (Apple Message framework v622) 14, 20 -- Message-Id: {5191556e91f285777b3d9c9740f74060-at-stonebow.otago.ac.nz} 14, 20 -- Content-Type: text/plain; charset=WINDOWS-1252; format=flowed 14, 20 -- To: microscopy-at-microscopy.com 14, 20 -- From: Allan Mitchell {allan.mitchell-at-stonebow.otago.ac.nz} 14, 20 -- Subject: [Microscopy] Birefringence of Fibers 14, 20 -- Date: Wed, 12 Oct 2005 16:49:09 +1300 14, 20 -- X-Mailer: Apple Mail (2.622) 14, 20 -- Content-Transfer-Encoding: 8bit 14, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9C3nDmG005664 ==============================End of - Headers==============================
I must admit I only use double (or is it dual*) sided DVD RAM which conveniently are naturally exactly 2x4.7GB and so really are 9.4Gb, but you do have to remove the cartridge and turn it over to get that extra 4.7Gb (remember the days of the 'B' side?). Of course once its formatted you get that Gb, bits and bytes thing where the actually capacity is always lower than 4.7Gb anyway.
I've never used an 8.5Gb dual layer DVD at £3.00+ when 4.7Gb DVD-R's are far cheaper at 9p and are the only format used by my Panasonic DVD-RAM multidrives at work (and Toshiba DVD RAM video recorder and PC's at home). I am adding dual layer DVD multiwriters (replacing the CD RW's) to co-exist with all my DVD-RAM cartridge drives as these can write standard DVD -R's at 16x and CD's at 30x or more (and to dual layer if required in the future). Optical drives are so incredibly cheap at the moment (and being so are definitely less reliable - so I always verify each burn).
http://www.dual-layer-dvd.co.uk/
Regards Keith
*But probably not doul
----- Original Message ----- X-from: {malcolm.haswell-at-sunderland.ac.uk} To: {keith.morris-at-ucl.ac.uk} Sent: Tuesday, October 11, 2005 5:57 PM
Ann,
There are programs that will do what you want. Metaxa is one (although expensive), and there very likely is an ImageJ plug-in. The more important question is: do you really want to do these measurements, or can they be done? There are several problems with measuring biological structures in the SEM: 1) Such measurements require stereopairs and appropriate trignonometry. These methods are well known by the photogrammetry people and are in the literature. 2) Fixation, dehydration, and drying all cause uncontrolled dimensional changes in structures. Further, as the samples sit in storage, they continue to shrink for some unknown time. And possibly can swell some amount when being moved through the air, picking up water from the atmosphere, into and out of the SEM chamber. 2a) These changes are dependent on the mechanical nature of the sample, and its physicochemical properties. This means that the dimensional changes will vary along the different axes as the sample makeup varies. Fibers for instance will behave differently along their length than they will across their width. This also applies to ultrastructure, since again the physicochemical properties are not identical in all directions. Plus, of course, the samples must have been handled *exactly* identically at all stages. So any differences in microvilli height are much more likely due to specimen processing and handling than to any intrinsic difference between e.g., cells. The possible exceptions are structures that are mechanically rigid enough to not be affected by the processing. Well-tanned insect cuticle and wood may fit this requirement. Calcified bone and teeth do. Cells don't. The only possible way around this particular problem is to do the work on properly cryofixed cells in a cryoSEM, and therefore not do any fixation, dehydration, etc. This means that unless the cells can be kept frozen in storage and never warmed much getting them into and out of the cryoSEM, the samples are only good when first examined. No going back to a sample. 3) I routinely see major differences in microvilli, such as presence/absence, and other surface ultrastructure on neighboring (as in right next to each other) cells. So, the cells were handled "*exactly* identically at all stages". But what does this mean? Most likely, just that the cells were at different metabolic stages. Like when looking at a section of epithelium with mucus-secreting cells. Different cells are at different stages of the secretory cycle, but it doesn't mean anything else. 4) The measurements are questionable anyway, unless the SEM is an expensive metrology instrument that has been carefully calibrated at all magnifications, working distances, tilts, etc. that are actually used. A non-metrology SEM is only good to +/- 5% accuracy at best -- if calibrated as above -- and more likely +/- 10%. Such measurements also depend on the local properties of the sample, which is highly affected by topography. The accuracy can be as low as +/- 20%. So relative statements, like "cell A's microvilli are about 120 nm +/- about X nm and cell B's microvilli are about 80 nm +/- about Y nm, so cell B's microvilli are approximately 2/3 the size of cell A' microvilli" are valid. A statement like "cell A's microvilli are 121.5 nm +/- 2 nm and cell B's microvilli are 79.3 nm +/- 3 nm" are invalid, even if measurements are made that precisely in stereopairs. The physics of imaging in the SEM isn't there, nor are the possible sources of error well enough known to allow such precision. Maybe in semiconductors or metals, but not in biological samples. 5) Feature brightness is correlated with height, but it is also correlated with many other properties of the sample. Local atomic and molecular composition, local density, local topography, etc., "local" meaning with the beam/specimen interaction volume. So, no, there is no direct, useful correlation between height and feature brightness. Although I'd be willing to bet that a particular sample (or type of sample) can be found where there is such a direct correlation, it won't be cellular ultrastructure.
Mike Bode's suggestion of something like a AFM is much more likely to give you the information you need. If the probe can be kept from ripping up the cells ...
Phil
I would like to measure height differences between microvilli on cultured cells that have been imaged by SEM. Is there a program available that does this type of measurement? Would Image J work? Is there a direct correlation between brightness of structure and height of surface structure that could be used in this manner to compare height of microvilli on adjacent cells? Any opinions would be welcomed.
Thanks,
Ann Tisdale Research Associate Schepens eye Research Institute Boston, MA 02114 email: tisdale-at-vision.eri.harvard.edu
==============================Original Headers============================== 4, 20 -- From tisdale-at-vision.eri.harvard.edu Tue Oct 11 10:31:38 2005 4, 20 -- Received: from vision.eri.harvard.edu (vision.eri.harvard.edu [128.103.8.12]) 4, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9BFVad7003038 4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 10:31:37 -0500 4, 20 -- Received: from vision (root-at-localhost) 4, 20 -- by vision.eri.harvard.edu (8.12.11/8.12.11) with SMTP id j9BFVXFc028381 4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 11:31:33 -0400 4, 20 -- Received: from [192.168.3.98] (e03-098.eri.harvard.edu [192.168.3.98]) 4, 20 -- by vision.eri.harvard.edu (8.12.11/8.12.11) with ESMTP id j9BFVWxY028367 4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 11 Oct 2005 11:31:32 -0400 4, 20 -- Mime-Version: 1.0 4, 20 -- Message-Id: {a06110400bf71855420de-at-[192.168.3.98]} 4, 20 -- Date: Tue, 11 Oct 2005 11:26:10 -0400 4, 20 -- To: microscopy-at-microscopy.com 4, 20 -- From: Ann Tisdale {tisdale-at-vision.eri.harvard.edu} 4, 20 -- Subject: SEM height measurements 4, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 20 -- X-SpamTest-Version: SMTP-Filter Version 2.0.0 [0122], KAS/Release 4, 20 -- X-Spamtest-Info: Pass through 4, 20 -- X-Anti-Virus: Kaspersky Anti-Virus for MailServers 5.5.2/RELEASE, bases: 11102005 #144202, status: clean ==============================End of - Headers==============================
==============================Original Headers============================== 11, 30 -- From oshel1pe-at-cmich.edu Wed Oct 12 08:00:35 2005 11, 30 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 11, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9CD0YgD029319 11, 30 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2005 08:00:35 -0500 11, 30 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 11, 30 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id j9CDiPwk024737 11, 30 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2005 09:44:26 -0400 11, 30 -- Received: from cmail4.central.cmich.local ([141.209.15.84]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 11, 30 -- Wed, 12 Oct 2005 09:00:32 -0400 11, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 11, 30 -- Content-class: urn:content-classes:message 11, 30 -- MIME-Version: 1.0 11, 30 -- Content-Type: text/plain; 11, 30 -- charset="iso-8859-1" 11, 30 -- Subject: RE: [Microscopy] SEM height measurements 11, 30 -- Date: Wed, 12 Oct 2005 09:00:27 -0400 11, 30 -- Message-ID: {07BD45BB3EE8A0468C94776E7A0C660E072E7A-at-cmail4.central.cmich.local} 11, 30 -- X-MS-Has-Attach: 11, 30 -- X-MS-TNEF-Correlator: 11, 30 -- Thread-Topic: [Microscopy] SEM height measurements 11, 30 -- Thread-Index: AcXOepaVx42p+L4YTi2iLlCAfIPtVwArNbyr 11, 30 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 11, 30 -- To: {tisdale-at-vision.eri.harvard.edu} 11, 30 -- Cc: {microscopy-at-microscopy.com} 11, 30 -- X-OriginalArrivalTime: 12 Oct 2005 13:00:32.0778 (UTC) FILETIME=[EDF64EA0:01C5CF2C] 11, 30 -- X-CanItPRO-Stream: default 11, 30 -- X-Spam-Score: 0.3 () FCS_URI_NODOTS 11, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 11, 30 -- Content-Transfer-Encoding: 8bit 11, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9CD0YgD029319 ==============================End of - Headers==============================
We have the volume, and would be happy to provide a copy of the article. We can photocopy it, or if preferred, we can scan it and send a PDF file. That might be a good option, as the last page is a color plate.
Please let me know what format you would like, and where to send it.
Regards,
Elaine
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
-----Original Message----- X-from: allan.mitchell-at-stonebow.otago.ac.nz [mailto:allan.mitchell-at-stonebow.otago.ac.nz] Sent: Tuesday, October 11, 2005 10:51 PM To: Elaine F. Schumacher
Hi all
A request from one of our Centre's users. Can anybody help out.
They are looking for the following article from The Microscope Journal ( - an international journal dedicated to the advancement of all forms of microscopy for the biologist, mineralogist, metallographer or chemist). Published Quarterly by McCrone Research Institute, Edited by Dr. Gary Laughlin.
They are after the following article: Gorski, A., and McCrone, W.C. "Birefringence of Fibers," THE MICROSCOPE, Vol.46:1, 1998, pp. 3-16
Very happy to pay for photocopying, postage....
Allan Mitchell Otago Centre for Electron Microscopy Department of Anatomy and Structural Biology School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254
EM Centre: http://ocem.otago.ac.nz/ Confocal Centre: http://occm.otago.ac.nz/ Department: http://anatomy.otago.ac.nz/
==============================Original Headers============================== 27, 27 -- From eschumacher-at-mccrone.com Wed Oct 12 08:38:38 2005 27, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122]) 27, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9CDccTT005889 27, 27 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2005 08:38:38 -0500 27, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 27, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 15B451A800B 27, 27 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2005 08:38:39 -0500 (CDT) 27, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 27, 27 -- by pgp.mccrone.com (PGP Universal service); 27, 27 -- Wed, 12 Oct 2005 08:38:39 -0500 27, 27 -- X-PGP-Universal: processed 27, 27 -- Content-class: urn:content-classes:message 27, 27 -- MIME-Version: 1.0 27, 27 -- Content-Type: text/plain; 27, 27 -- charset="US-ASCII" 27, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 27, 27 -- Subject: RE: [Microscopy] Birefringence of Fibers 27, 27 -- Date: Wed, 12 Oct 2005 08:38:37 -0500 27, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A74C3002-at-MCCRONEMSG.tmg.mccrone.com} 27, 27 -- X-MS-Has-Attach: 27, 27 -- X-MS-TNEF-Correlator: 27, 27 -- Thread-Topic: [Microscopy] Birefringence of Fibers 27, 27 -- thread-index: AcXO4Cavo45akdyHQV28X6zuRzhzgwAUZPkw 27, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 27, 27 -- To: {allan.mitchell-at-stonebow.otago.ac.nz} , {microscopy-at-microscopy.com} 27, 27 -- Content-Transfer-Encoding: 8bit 27, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9CDccTT005889 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pgan-at-ap.ansell.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, October 11, 2005 at 22:05:47 ---------------------------------------------------------------------------
Email: pgan-at-ap.ansell.com Name: Phay Fang Gan
Organization: Ansell
Education: Graduate College
Location: Shah Alam, selangor, Malaysia
Question: I was once asked by a consultant whether gold/palladium or gold coating would affect the surface morphology of a specimen coated. Appreciate much all your professional advice
As an addendum to my previous posting, if no one else has responded, and we are asked to provide a copy, we would of course get permission from the McCrone Research Institute to do so, or they could be contacted directly.
Regards,
Elaine
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
-----Original Message----- X-from: allan.mitchell-at-stonebow.otago.ac.nz [mailto:allan.mitchell-at-stonebow.otago.ac.nz] Sent: Tuesday, October 11, 2005 10:51 PM To: Elaine F. Schumacher
Hi all
A request from one of our Centre's users. Can anybody help out.
They are looking for the following article from The Microscope Journal ( - an international journal dedicated to the advancement of all forms of microscopy for the biologist, mineralogist, metallographer or chemist). Published Quarterly by McCrone Research Institute, Edited by Dr. Gary Laughlin.
They are after the following article: Gorski, A., and McCrone, W.C. "Birefringence of Fibers," THE MICROSCOPE, Vol.46:1, 1998, pp. 3-16
Very happy to pay for photocopying, postage....
Allan Mitchell Otago Centre for Electron Microscopy Department of Anatomy and Structural Biology School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254
EM Centre: http://ocem.otago.ac.nz/ Confocal Centre: http://occm.otago.ac.nz/ Department: http://anatomy.otago.ac.nz/
==============================Original Headers============================== 26, 27 -- From eschumacher-at-mccrone.com Wed Oct 12 09:03:07 2005 26, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122]) 26, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9CE37mh023405 26, 27 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2005 09:03:07 -0500 26, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 26, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 998C01A800B 26, 27 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2005 09:03:08 -0500 (CDT) 26, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 26, 27 -- by pgp.mccrone.com (PGP Universal service); 26, 27 -- Wed, 12 Oct 2005 09:03:08 -0500 26, 27 -- X-PGP-Universal: processed 26, 27 -- Content-class: urn:content-classes:message 26, 27 -- MIME-Version: 1.0 26, 27 -- Content-Type: text/plain; 26, 27 -- charset="US-ASCII" 26, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 26, 27 -- Subject: RE: [Microscopy] Birefringence of Fibers 26, 27 -- Date: Wed, 12 Oct 2005 09:02:56 -0500 26, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A74C3005-at-MCCRONEMSG.tmg.mccrone.com} 26, 27 -- X-MS-Has-Attach: 26, 27 -- X-MS-TNEF-Correlator: 26, 27 -- Thread-Topic: [Microscopy] Birefringence of Fibers 26, 27 -- thread-index: AcXO4Cavo45akdyHQV28X6zuRzhzgwAVNuXA 26, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 26, 27 -- To: {allan.mitchell-at-stonebow.otago.ac.nz} , {microscopy-at-microscopy.com} 26, 27 -- Content-Transfer-Encoding: 8bit 26, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9CE37mh023405 ==============================End of - Headers==============================
The coating itself won't affect the surface morphology, although the coating process might. Sputter coating can heat specimens. Normally, this isn't a problem, since sputter coaters generally are designed to keep the electrons, which do most of the heating, away from the samples. But for low melting-point samples, such as bloom on chocolate, even short bursts of coating with melt the surface. As I found out from experience. But, at high magnification, say 30,000X and above, the "surface morphology" of the sample is modified in the sense that the structure of the coating becomes visible. Pure gold produces a lumpier coat than say 60/40 gold/palladium, and since Au/Pd is cheaper than pure Au, I find it best to just use Au/Pd targets.
Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University Mt. Pleasant, MI 48859
Email: pgan-at-ap.ansell.com Name: Phay Fang Gan
Organization: Ansell
Education: Graduate College
Location: Shah Alam, selangor, Malaysia
Question: I was once asked by a consultant whether gold/palladium or gold coating would affect the surface morphology of a specimen coated. Appreciate much all your professional advice
==============================Original Headers============================== 11, 30 -- From oshel1pe-at-cmich.edu Wed Oct 12 09:24:02 2005 11, 30 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 11, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9CEO1Ll032285 11, 30 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2005 09:24:01 -0500 11, 30 -- Received: from egateb.central.cmich.local ([141.209.15.85]) 11, 30 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id j9CF7WxS030977 11, 30 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2005 11:07:55 -0400 11, 30 -- Received: from cmail4.central.cmich.local ([141.209.15.84]) by egateb.central.cmich.local with Microsoft SMTPSVC(5.0.2195.6713); 11, 30 -- Wed, 12 Oct 2005 10:22:47 -0400 11, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 11, 30 -- Content-class: urn:content-classes:message 11, 30 -- MIME-Version: 1.0 11, 30 -- Content-Type: text/plain; 11, 30 -- charset="iso-8859-1" 11, 30 -- Subject: RE: [Microscopy] [Filtered] AskAMicroscopist: gold/palladium or gold coating 11, 30 -- Date: Wed, 12 Oct 2005 10:22:44 -0400 11, 30 -- Message-ID: {07BD45BB3EE8A0468C94776E7A0C660E072E7E-at-cmail4.central.cmich.local} 11, 30 -- X-MS-Has-Attach: 11, 30 -- X-MS-TNEF-Correlator: 11, 30 -- Thread-Topic: [Microscopy] [Filtered] AskAMicroscopist: gold/palladium or gold coating 11, 30 -- Thread-Index: AcXPM+TZi0GfCQs6TvuyIBok7Ww5fAAA8SYF 11, 30 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 11, 30 -- To: {pgan-at-ap.ansell.com} 11, 30 -- Cc: {microscopy-at-microscopy.com} 11, 30 -- X-OriginalArrivalTime: 12 Oct 2005 14:22:47.0494 (UTC) FILETIME=[6B483660:01C5CF38] 11, 30 -- X-CanItPRO-Stream: default 11, 30 -- X-Spam-Score: 0 () 11, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 11, 30 -- Content-Transfer-Encoding: 8bit 11, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9CEO1Ll032285 ==============================End of - Headers==============================
Is there any disadvantage in moving from Au to Au/Pd targets eg vacuum requirements or quality/quantity of coating for low magnification applications?
Dave
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: 12 October 2005 15:28 To: David Patton
The coating itself won't affect the surface morphology, although the coating process might. Sputter coating can heat specimens. Normally, this isn't a problem, since sputter coaters generally are designed to keep the electrons, which do most of the heating, away from the samples. But for low melting-point samples, such as bloom on chocolate, even short bursts of coating with melt the surface. As I found out from experience. But, at high magnification, say 30,000X and above, the "surface morphology" of the sample is modified in the sense that the structure of the coating becomes visible. Pure gold produces a lumpier coat than say 60/40 gold/palladium, and since Au/Pd is cheaper than pure Au, I find it best to just use Au/Pd targets.
Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University Mt. Pleasant, MI 48859
Email: pgan-at-ap.ansell.com Name: Phay Fang Gan
Organization: Ansell
Education: Graduate College
Location: Shah Alam, selangor, Malaysia
Question: I was once asked by a consultant whether gold/palladium or gold coating would affect the surface morphology of a specimen coated. Appreciate much all your professional advice
==============================Original Headers============================== 11, 30 -- From oshel1pe-at-cmich.edu Wed Oct 12 09:24:02 2005 11, 30 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 11, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9CEO1Ll032285 11, 30 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2005 09:24:01 -0500 11, 30 -- Received: from egateb.central.cmich.local ([141.209.15.85]) 11, 30 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id j9CF7WxS030977 11, 30 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2005 11:07:55 -0400 11, 30 -- Received: from cmail4.central.cmich.local ([141.209.15.84]) by egateb.central.cmich.local with Microsoft SMTPSVC(5.0.2195.6713); 11, 30 -- Wed, 12 Oct 2005 10:22:47 -0400 11, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 11, 30 -- Content-class: urn:content-classes:message 11, 30 -- MIME-Version: 1.0 11, 30 -- Content-Type: text/plain; 11, 30 -- charset="iso-8859-1" 11, 30 -- Subject: RE: [Microscopy] [Filtered] AskAMicroscopist: gold/palladium or gold coating 11, 30 -- Date: Wed, 12 Oct 2005 10:22:44 -0400 11, 30 -- Message-ID: {07BD45BB3EE8A0468C94776E7A0C660E072E7E-at-cmail4.central.cmich.local} 11, 30 -- X-MS-Has-Attach: 11, 30 -- X-MS-TNEF-Correlator: 11, 30 -- Thread-Topic: [Microscopy] [Filtered] AskAMicroscopist: gold/palladium or gold coating 11, 30 -- Thread-Index: AcXPM+TZi0GfCQs6TvuyIBok7Ww5fAAA8SYF 11, 30 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 11, 30 -- To: {pgan-at-ap.ansell.com} 11, 30 -- Cc: {microscopy-at-microscopy.com} 11, 30 -- X-OriginalArrivalTime: 12 Oct 2005 14:22:47.0494 (UTC) FILETIME=[6B483660:01C5CF38] 11, 30 -- X-CanItPRO-Stream: default 11, 30 -- X-Spam-Score: 0 () 11, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 11, 30 -- Content-Transfer-Encoding: 8bit 11, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9CEO1Ll032285 ==============================End of - Headers==============================
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==============================Original Headers============================== 26, 34 -- From David.Patton-at-uwe.ac.uk Wed Oct 12 09:33:12 2005 26, 34 -- Received: from mailapp01.uwe.ac.uk (mailapp01.uwe.ac.uk [164.11.132.61]) 26, 34 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9CEXBf2008542 26, 34 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2005 09:33:12 -0500 26, 34 -- Received: from (164.11.132.62) by mailapp01.uwe.ac.uk via smtp 26, 34 -- id 2f3c_3361fba8_3b2d_11da_83fe_0002b3c946e4; 26, 34 -- Wed, 12 Oct 2005 15:33:51 +0100 26, 34 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 26, 34 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121]) 26, 34 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 26, 34 -- 2005)) with ESMTP id {0IO900K5A4F5K3-at-mta02.uwe.ac.uk} for 26, 34 -- microscopy-at-microscopy.com; Wed, 12 Oct 2005 15:33:05 +0100 (BST) 26, 34 -- Date: Wed, 12 Oct 2005 15:33:06 +0100 26, 34 -- From: David Patton {David.Patton-at-uwe.ac.uk} 26, 34 -- Subject: RE: [Microscopy] RE: [Filtered] AskAMicroscopist: gold/palladium or 26, 34 -- gold coating 26, 34 -- To: microscopy-at-microscopy.com 26, 34 -- Message-id: {F247F674896BE243AD8263C5280E2BDBF84DD5-at-NBUEGEN01} 26, 34 -- MIME-version: 1.0 26, 34 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5.6944.0 26, 34 -- Content-type: text/plain; charset=us-ascii 26, 34 -- Content-class: urn:content-classes:message 26, 34 -- Thread-topic: [Microscopy] RE: [Filtered] AskAMicroscopist: gold/palladium or 26, 34 -- gold coating 26, 34 -- Thread-index: AcXPOS4DHKuH20VrSy2zh5KMF3b1sAAADyuA 26, 34 -- X-MS-Has-Attach: 26, 34 -- X-MS-TNEF-Correlator: 26, 34 -- X-NAI-Spam-Score: -0.3 26, 34 -- X-NAI-Spam-Rules: 1 Rules triggered 26, 34 -- BAYES_30=-0.3 26, 34 -- X-NAIMIME-Disclaimer: 1 26, 34 -- X-NAIMIME-Modified: 1 26, 34 -- Content-Transfer-Encoding: 8bit 26, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9CEXBf2008542 ==============================End of - Headers==============================
No. The Au/Pd targets work the same as pure Au targets, and I use the same coating parameters. If you use a coating thickness monitor, like a quartz-crystal instrument, you have to change the work function and mass in the programming. If a thickness monitor isn't used, then I don't find any need for changes. The Au/Pd coating at low mags is as good as gold.
Phil
Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University Mt. Pleasant, MI 48859
-----Original Message----- X-from: Oshel, Philip Eugene Sent: Wed 12-Oct-05 10:38 To: David.Patton-at-uwe.ac.uk
The coating itself won't affect the surface morphology, although the coating process might. Sputter coating can heat specimens. Normally, this isn't a problem, since sputter coaters generally are designed to keep the electrons, which do most of the heating, away from the samples. But for low melting-point samples, such as bloom on chocolate, even short bursts of coating with melt the surface. As I found out from experience. But, at high magnification, say 30,000X and above, the "surface morphology" of the sample is modified in the sense that the structure of the coating becomes visible. Pure gold produces a lumpier coat than say 60/40 gold/palladium, and since Au/Pd is cheaper than pure Au, I find it best to just use Au/Pd targets.
Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University Mt. Pleasant, MI 48859
Email: pgan-at-ap.ansell.com Name: Phay Fang Gan
Organization: Ansell
Education: Graduate College
Location: Shah Alam, selangor, Malaysia
Question: I was once asked by a consultant whether gold/palladium or gold coating would affect the surface morphology of a specimen coated. Appreciate much all your professional advice
==============================Original Headers============================== 33, 29 -- From oshel1pe-at-cmich.edu Wed Oct 12 09:42:57 2005 33, 29 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 33, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9CEgvGK017292 33, 29 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2005 09:42:57 -0500 33, 29 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 33, 29 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id j9CFQowi032574 33, 29 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2005 11:26:50 -0400 33, 29 -- Received: from cmail4.central.cmich.local ([141.209.15.84]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 33, 29 -- Wed, 12 Oct 2005 10:42:56 -0400 33, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 33, 29 -- Content-class: urn:content-classes:message 33, 29 -- MIME-Version: 1.0 33, 29 -- Content-Type: text/plain; 33, 29 -- charset="iso-8859-1" 33, 29 -- Subject: RE: [Microscopy] [Filtered] AskAMicroscopist: gold/palladium or 33, 29 -- Date: Wed, 12 Oct 2005 10:42:15 -0400 33, 29 -- Message-ID: {07BD45BB3EE8A0468C94776E7A0C660E072E83-at-cmail4.central.cmich.local} 33, 29 -- X-MS-Has-Attach: 33, 29 -- X-MS-TNEF-Correlator: 33, 29 -- Thread-Topic: [Microscopy] [Filtered] AskAMicroscopist: gold/palladium or 33, 29 -- Thread-Index: AcXPOouiCkb6X+guS32LuNNgfrftqQAAAz/PAAAiqnE= 33, 29 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 33, 29 -- To: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} , {microscopy-at-microscopy.com} 33, 29 -- X-OriginalArrivalTime: 12 Oct 2005 14:42:56.0848 (UTC) FILETIME=[3C1CFD00:01C5CF3B] 33, 29 -- X-CanItPRO-Stream: default 33, 29 -- X-Spam-Score: 0 () 33, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 33, 29 -- Content-Transfer-Encoding: 8bit 33, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9CEgvGK017292 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sachep-at-rockefeller.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, October 13, 2005 at 04:28:56 ---------------------------------------------------------------------------
Question: What incubation media should I use to perform live cell imaging of cells in an open chamber. ie. buffer conditions HEPES concentration etc. Thank you
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (vincent.metzger-at-philips.com) from http://www.microscopy.com/MLFormMail.html on Thursday, October 13, 2005 at 07:54:07 ---------------------------------------------------------------------------
Email: vincent.metzger-at-philips.com Name: vincent metzger
Question: I would like to study the boundary grain of Wfilament of lamp. I'm searching a way to prepare the sample to make good observation of the grain boundary.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (curt-at-oxfordenvironment.co.uk) from http://www.microscopy.com/MLFormMail.html on Wednesday, October 12, 2005 at 02:59:17 ---------------------------------------------------------------------------
Title-Subject: [Filtered] MListserver: M7A Yellow blue edging on image
Question: Hello,
I have a Wild M7A stereomicroscope and the image(s) are edged with yellow on one side and, if you close one eye, blue on the other. This causes a yellow cast to object edges and is very annoying.
I am using either x20 or x10 eyepieces, with LED or f-optic ring illumination. The problem is most obvious under higher magnifications.
Does anyone know why this happens, and how it can be solved?
Greetings, All! We are in the search of a 3D reconstruction from tilt series software from EM for our undergraduate students. Would you be willing to share your experience on various options and programs. The most basic and generic (although addressing register alignment and missing cone), the most desired. Vendors welcome.
==============================Original Headers============================== 2, 26 -- From mmalecki-at-wisc.edu Thu Oct 13 08:59:35 2005 2, 26 -- Received: from smtp7.wiscmail.wisc.edu (hagen.doit.wisc.edu [144.92.197.163]) 2, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DDxZxu023302 2, 26 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 08:59:35 -0500 2, 26 -- Received: from avs-daemon.smtp7.wiscmail.wisc.edu by smtp7.wiscmail.wisc.edu 2, 26 -- (iPlanet Messaging Server 5.2 HotFix 2.04 (built Feb 8 2005)) 2, 26 -- id {0IOA00I31XJA8O-at-smtp7.wiscmail.wisc.edu} for microscopy-at-microscopy.com; 2, 26 -- Thu, 13 Oct 2005 08:59:34 -0500 (CDT) 2, 26 -- Received: from marek-78c5126ca.wisc.edu 2, 26 -- (24-196-111-161.dhcp.mdsn.wi.charter.com [24.196.111.161]) 2, 26 -- by smtp7.wiscmail.wisc.edu 2, 26 -- (iPlanet Messaging Server 5.2 HotFix 2.04 (built Feb 8 2005)) 2, 26 -- with ESMTPSA id {0IOA00EZ0XJ9R1-at-smtp7.wiscmail.wisc.edu} for 2, 26 -- microscopy-at-microscopy.com; Thu, 13 Oct 2005 08:59:33 -0500 (CDT) 2, 26 -- Date: Thu, 13 Oct 2005 09:01:27 -0500 2, 26 -- From: Marek Malecki MD PhD {mmalecki-at-wisc.edu} 2, 26 -- Subject: search for the 3D reconstruction from EM tilt series software 2, 26 -- To: microscopy-at-microscopy.com 2, 26 -- Message-id: {6.2.3.4.0.20051013085234.01e8a9b0-at-wiscmail.wisc.edu} 2, 26 -- MIME-version: 1.0 2, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 2, 26 -- Content-type: text/plain; format=flowed; charset=us-ascii 2, 26 -- Content-transfer-encoding: 7BIT 2, 26 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=24.196.111.161 2, 26 -- X-Spam-PmxInfo: Server=avs-2, Version=5.0.3.165339, Antispam-Engine: 2.1.0.0, 2, 26 -- Antispam-Data: 2005.10.13.11, SenderIP=24.196.111.161 ==============================End of - Headers==============================
Confocal Microscopist Center for Advanced Microscopy Michigan State University
Full-time confocal microscopist. This is a teaching/service/support position at the Center for Advanced Microscopy at Michigan State University. CAM is the central microscopy laboratory for the MSU campus, serving users from a wide variety of disciplines. The appointment will be in the Academic Specialist category, one of the academic support ranks at the University. The appointment will be a 12-month, annual year appointment in the continuing appointment system. Salary will be commensurate with experience.
Requirements include: a PhD degree in the biological sciences and a minimum of three years experience with confocal microscopy instrumentation including theory, operation, and maintenance. Experience in preparing and imaging fixed and living biological tissue is required. Experience with immunohistochemistry, Fluorescence Resonance Energy Transfer (FRET), Fluorescence Loss in Photobleaching (FLIP) and Fluorescence Recovery After Photobleaching (FRAP) is highly desirable as is knowledge of multi-user facility operation. The individual must have a demonstrated competence in and a strong commitment to graduate and post-graduate instruction and should assist others in planning their microscopy research programs and/or sample preparation.
The individual selected will supervise a newly funded spectral confocal microscope, as well as two existing confocal microscopes. U.S. citizenship is not required; applicants who are not U.S. citizens or permanent residents must provide documentation evidencing employment authorization in the United States. The position begins effective Spring 2006.
Submit a curriculum vita, transcripts of academic training, a statement describing your interest in the position, evidence of teaching ability, and arrange for three letters of recommendation to be sent to: Dr. Stanley L. Flegler, Chair, Search Committee, Center for Advanced Microscopy, B4 CIPS Bldg, Michigan State University, East Lansing, MI 48824, U.S.A. Applications are due by December 1, 2005.
MSU is an Affirmative Action/Equal Opportunity Institution
==============================Original Headers============================== 8, 19 -- From flegler-at-msu.edu Thu Oct 13 09:06:10 2005 8, 19 -- Received: from sys35.mail.msu.edu (sys35.mail.msu.edu [35.9.75.135]) 8, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DE69Kq031976 8, 19 -- for {microscopy-at-Microscopy.com} ; Thu, 13 Oct 2005 09:06:10 -0500 8, 19 -- Received: from [35.8.77.201] (helo=CAMKOLN.msu.edu) 8, 19 -- by sys35.mail.msu.edu with esmtpsa (Exim 4.52 #1) 8, 19 -- (TLSv1:RC4-SHA:128) 8, 19 -- id 1EQ3ij-0003qP-Mu 8, 19 -- for microscopy-at-Microscopy.com; Thu, 13 Oct 2005 10:06:09 -0400 8, 19 -- Message-Id: {6.0.3.0.2.20051013100426.02037e40-at-mail.msu.edu} 8, 19 -- X-Sender: flegler-at-mail.msu.edu 8, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 6.0.3.0 8, 19 -- Date: Thu, 13 Oct 2005 10:05:43 -0400 8, 19 -- To: microscopy-at-Microscopy.com 8, 19 -- From: "Stanley L. Flegler" {flegler-at-msu.edu} 8, 19 -- Subject: LM Job Posting 8, 19 -- Mime-Version: 1.0 8, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 19 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
I just created a new platform for us to discuss microscopy online using Web Wiz Forums. It might be a better platform than listservers, especially for those who want to reduce unnecessary emails. (I am not intending to say this listserv is bad. In fact Nestor did a great job and the listserv has served us for more than 10 years.) With the new online forum you can still set email notification for the topics you are interested in, at the same time you may ignore the topics out of interest.
Other features of this online discussion board include: ***You need NOT register to post messages in most of the forums. However you have to type in your name every time you post a message. Registered users have full access to the website contents and functionality. ***Students or any Microscopists may ask questions related to microscopy in the Discussion board. ***Employers may post vacant positions in the Jobs & Resumes. ***Job-hunters are welcome to post their resumes too. ***Users may post and discuss their recent publications in recommended Readings. ***Open facilities or EM consulting companies may post their contact information in EM facilities nearby. ***Software authors may post their beta tests or freewares/sharewares in Software downloads. ***Commercial companies may post their product information and contact information free in EM companies ***I am collecting Daily Operation Manuals for all kinds of microscopes. Please send me your contributions to me. I will include your name as courtesy. After I have collected sufficient amount of manuals I will put them here so our users may benefit from your kindness. ***Finally but not least, you may create your own customized interest group in Customized Discussion Groups. You may set it as access-by-authorization-only or open to everyone.
The link to this forum is http://www.ShuyouLi.com/
I welcome all kinds of comments, suggestions and criticisms - to make us microscopists feel more convenient to discuss virtually world wide.
Thanks, Shuyou Li
_____________________________ Shu-You Li, Ph.D. Electron Microscopist, EPIC NUANCE Northwestern University 2220 Campus Drive, 1161 Cook Hall (#2036 for mail) Evanston, IL 60208-3108, USA Ph: (847) 491-6723(O), (847) 491-7806(L), Fax: (847) 467-6573 Email: syli-at-northwestern.edu; shuyouli-at-gmail.com http://www.nuance.northwestern.edu/EPIC http://www.shuyouli.com
==============================Original Headers============================== 10, 16 -- From syli-at-northwestern.edu Thu Oct 13 09:55:41 2005 10, 16 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57]) 10, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DEtfPA008880 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 09:55:41 -0500 10, 16 -- Received: from [129.105.37.16] (andromeda.ms.northwestern.edu [129.105.37.16]) 10, 16 -- by merle.it.northwestern.edu (Postfix) with ESMTP id EE7109E847 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 09:55:40 -0500 (CDT) 10, 16 -- Date: Thu, 13 Oct 2005 09:57:37 -0500 10, 16 -- From: Shu-You Li {syli-at-northwestern.edu} 10, 16 -- To: microscopy-at-microscopy.com 10, 16 -- Subject: Anew platform for microscopists to discuss microscopy online 10, 16 -- Message-Id: {20051013095630.0382.SYLI-at-northwestern.edu} 10, 16 -- MIME-Version: 1.0 10, 16 -- Content-Type: text/plain; charset="US-ASCII" 10, 16 -- Content-Transfer-Encoding: 7bit 10, 16 -- X-Mailer: Becky! ver. 2.20 [en] ==============================End of - Headers==============================
I have a question for all thoughs that have done immunogold labelling on cryothin sections. I am having trouble getting consistant, predictable results. Meaning that my signal to noise seems variable which makes it difficult to standardize the protocol. One thing that I have noticed is a 5-15x increase in signal (based on gold count) if I use just BSA and omit any normal serum. I also see an increase in background but in seemingly random patterns. (Sometimes I think the positives are much cleaner than the minus primary controls). The protocol that I am using at present is: place freshly picked up sections on droplet of 0.05M glycine / 1% BSA in TBS (1 hr), then 1% BSA in TBS (1 hr), primary antibody overnight (1% BSA/TBS), secondary ( ultrasmall up to 20nm gold) for (2 hr), fix, embed and view. My previous standard protocol was including 5-10% normal serum in all the above solutions. Has anyone else seen this increase in labelling frequency? I would be interested in protocols that people are very pleased with and give consistant clean results. Bottom line: Have any of you found any key tricks to getting the immunogold labelling of cryothin sections to perform without the "hitch".
Thank you for any help!
Robert Underwood Sr. Research Scientist U of Washington
==============================Original Headers============================== 5, 20 -- From underwoo-at-u.washington.edu Thu Oct 13 10:24:40 2005 5, 20 -- Received: from mxout2.cac.washington.edu (mxout2.cac.washington.edu [140.142.33.4]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DFOdgw017788 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 13 Oct 2005 10:24:39 -0500 5, 20 -- Received: from hymn10.u.washington.edu (hymn10.u.washington.edu [140.142.13.244]) 5, 20 -- by mxout2.cac.washington.edu (8.13.4+UW05.04/8.13.4+UW05.09) with ESMTP id j9DFOciU013706 5, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 13 Oct 2005 08:24:38 -0700 5, 20 -- Received: from localhost (localhost [127.0.0.1]) 5, 20 -- by hymn10.u.washington.edu (8.13.4+UW05.04/8.13.4+UW05.09) with ESMTP id j9DFOcs7031737 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 13 Oct 2005 08:24:38 -0700 5, 20 -- Received: from [140.142.8.53] by hymn10.u.washington.edu via HTTP; Thu, 13 Oct 2005 08:24:38 PDT 5, 20 -- Date: Thu, 13 Oct 2005 08:24:38 -0700 (PDT) 5, 20 -- From: Robert A Underwood {underwoo-at-u.washington.edu} 5, 20 -- To: Microscopy List {Microscopy-at-MSA.Microscopy.Com} 5, 20 -- Subject: [Microscopy] Cryothin Immunogold? 5, 20 -- Message-ID: {Pine.LNX.4.43.0510130824380.28728-at-hymn10.u.washington.edu} 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 5, 20 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
Very generous of you to say that you don't intend to say that the microscopy listserver is bad.
The last thing I need is another forum sending me email. Thanks, but no thanks. I'm sticking with Nestor where I can expect another decade or two of continuity!
Ron Anderson
syli-at-northwestern.edu wrote:
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==============================Original Headers============================== 7, 21 -- From randerson20-at-tampabay.rr.com Thu Oct 13 11:01:50 2005 7, 21 -- Received: from ms-smtp-04.tampabay.rr.com (ms-smtp-04-smtplb.tampabay.rr.com [65.32.5.134]) 7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DG1nep003055 7, 21 -- for {Microscopy-at-Microscopy.Com} ; Thu, 13 Oct 2005 11:01:50 -0500 7, 21 -- Received: from [127.0.0.1] (rrcs-24-73-73-214.se.biz.rr.com [24.73.73.214]) 7, 21 -- by ms-smtp-04.tampabay.rr.com (8.12.10/8.12.7) with ESMTP id j9DG1kai006840; 7, 21 -- Thu, 13 Oct 2005 12:01:47 -0400 (EDT) 7, 21 -- Message-ID: {434E84E7.4020706-at-tampabay.rr.com} 7, 21 -- Date: Thu, 13 Oct 2005 12:01:43 -0400 7, 21 -- From: Ronald Anderson {randerson20-at-tampabay.rr.com} 7, 21 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 7, 21 -- X-Accept-Language: en-us, en 7, 21 -- MIME-Version: 1.0 7, 21 -- To: syli-at-northwestern.edu, Listserver {Microscopy-at-Microscopy.Com} 7, 21 -- Subject: Re: [Microscopy] Anew platform for microscopists to discuss microscopy 7, 21 -- online 7, 21 -- References: {200510131455.j9DEtpcG009106-at-ns.microscopy.com} 7, 21 -- In-Reply-To: {200510131455.j9DEtpcG009106-at-ns.microscopy.com} 7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
Good afternoon, dear Robert, as normally virus(ses) or virus suspension have to be "inactivated" by formaldehyde for handling, mailing and processing (at least in a BSL2-category labs, see, e.g., http://www.d.umn.edu/ehso/biosafety/bsl2.html and see also specific recommendations at
or see the additional separate articles on that site ),
I think that the fixation already has been 100% and finished for the "particles" and you then are dealing only with the "remaining" fluid.
Since there is nothing left for further fixation (of virus particles, because they ARE fixed!) then, only excess formaldehyde-containing solution has to be removed. This can be easily done by spilling grids (with previousely absorbed virus particles) with Aqua (tri-,bidest), in the case you have to remove fixative from e.g. Eppendorf's (containing virus supension in Formaldehyde solution) you are to "wash" the microtubes for several times (after each step you have to spin down virus particles and for such you need perhaps } an airfuge { to get the high g-forces needed).
Perhaps another possibility (I am not aware nor have tried): perhaps filtering or using the agar-concentraion technique.... Unfortunately you have not told us the reason for you have to get rid of the excess formaldehyde portion....
Best wishes (and hopefully there are a lot of other interesting considerations),
regards, Wolfgang Muss Salzburg, Austria
OR Dr. Wolfgang Muss Member of EM-Lab Institute of Pathology, SALK (Salzburger Landeskliniken gemeinnuetzige GesmbH) Muellner Hauptstrasse 48 A-5020 SALZBURG AUSTRIA/EUROPE
and/or/alternatively
Paracelsus Medical Private University (PMU) Institute of Pathology Electron Microscopy Lab Muellner Hauptstrasse 48 A-5020 SALZBURG, Austria/Europe Phone work: +43+662+4482+4720 Mobile phone work:+43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss") E-Mail work: W.Muss-at-SALK.at
---------- Von: derby-at-nmt.edu[SMTP:derby-at-nmt.edu] Antwort an: derby-at-nmt.edu Gesendet: Donnerstag, 13. Oktober 2005 17:52 An: W.Muss-at-salk.at Betreff: [Microscopy] How to remove formalin
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Greetings to all,
I am a bit stumped. I have been asked to find a way to remove formalin, from formalin fixed viruses. Any tips/trick/methods would be appreciated.
Thank you,
Robert Derby New Mexico Tech.
==============================Original Headers============================== 5, 16 -- From derby-at-nmt.edu Thu Oct 13 10:46:56 2005 5, 16 -- Received: from mailhost.nmt.edu (mailhost.NMT.EDU [129.138.4.52]) 5, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DFku5v026743 5, 16 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 13 Oct 2005 10:46:56 -0500 5, 16 -- Received: from [129.138.14.28] (robert.nmt.edu [129.138.14.28]) 5, 16 -- by mailhost.nmt.edu (8.13.0/8.13.0) with ESMTP id j9DFkrEq014820 5, 16 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 13 Oct 2005 09:46:54 -0600 5, 16 -- User-Agent: Microsoft-Outlook-Express-Macintosh-Edition/5.02.2022 5, 16 -- Date: Thu, 13 Oct 2005 09:47:45 -0600 5, 16 -- Subject: How to remove formalin 5, 16 -- From: Robert {derby-at-nmt.edu} 5, 16 -- To: {Microscopy-at-msa.microscopy.com} 5, 16 -- Message-ID: {BF73DDC1.357%derby-at-nmt.edu} 5, 16 -- Mime-version: 1.0 5, 16 -- Content-type: text/plain; charset="US-ASCII" 5, 16 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 25, 28 -- From W.Muss-at-salk.at Thu Oct 13 11:20:27 2005 25, 28 -- Received: from Hermes.salk.at (hermes.lks.at [193.170.167.9]) 25, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DGKQDU011938 25, 28 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 13 Oct 2005 11:20:26 -0500 25, 28 -- Received: from localhost (localhost [127.0.0.1]) 25, 28 -- by hermes.lks.at (Postfix) with ESMTP id 493065A9031; 25, 28 -- Thu, 13 Oct 2005 18:20:24 +0200 (CEST) 25, 28 -- Received: from hermes.lks.at ([127.0.0.1]) 25, 28 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024) 25, 28 -- with ESMTP id 53965-03; Thu, 13 Oct 2005 18:20:23 +0200 (CEST) 25, 28 -- Received: from c1pa003 (unknown [192.168.42.3]) 25, 28 -- by hermes.lks.at (Postfix) with SMTP id B70CB5A902A; 25, 28 -- Thu, 13 Oct 2005 18:20:23 +0200 (CEST) 25, 28 -- Received: by localhost with Microsoft MAPI; Thu, 13 Oct 2005 18:20:19 +0200 25, 28 -- Message-ID: {01C5D022.C4B83840.W.Muss-at-salk.at} 25, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at} 25, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at} 25, 28 -- To: "'derby-at-nmt.edu'" {derby-at-nmt.edu} 25, 28 -- Cc: "'Microscopy-at-msa.microscopy.com'" {Microscopy-at-msa.microscopy.com} 25, 28 -- Subject: AW: [Microscopy] How to remove formalin 25, 28 -- Date: Thu, 13 Oct 2005 18:20:18 +0200 25, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at} 25, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor 25, 28 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211 25, 28 -- MIME-Version: 1.0 25, 28 -- Content-Type: text/plain; charset="us-ascii" 25, 28 -- Content-Transfer-Encoding: 7bit 25, 28 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at ==============================End of - Headers==============================
We have very consistent results with a different protocol. Check it out on our website (protocol 7): http://cellserv.med.yale.edu/imaging/ccmi/elect_protocols.html
The main differences are:
- We use 0.1 M NH4Cl instead of glycin, but that should not matter much. - We use 1% of fish skin gelatin instead of BSA. - We use PBS instead of TBS, but again that should not really matter - Incubations with antibodies and protein A-gold are for 30 min. This could be an important factor. Why do you incubate so long with antibodies? Maybe when antibodies don't work too well it would make sense, but I wouldn't do this systematically. This is bound to create problems with aggregation of antibodies, increased background, etc. Also, we only block with NH4Cl and fish skin gelatin for a maximum of 10 and 20 mins respectively. This means most of our immunolabelings are completed within 2 hours! Much more cost-efficient I would say.
Good luck
Marc
On Oct 13, 2005, at 11:25 AM, underwoo-at-u.washington.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hello All, } } I have a question for all thoughs that have done immunogold } labelling on } cryothin sections. I am having trouble getting consistant, } predictable results. } Meaning that my signal to noise seems variable which makes it } difficult to } standardize the protocol. One thing that I have noticed is a 5-15x } increase in } signal (based on gold count) if I use just BSA and omit any normal } serum. I } also see an increase in background but in seemingly random } patterns. (Sometimes } I think the positives are much cleaner than the minus primary } controls). } The protocol that I am using at present is: place freshly picked up } sections on } droplet of 0.05M glycine / 1% BSA in TBS (1 hr), then 1% BSA in TBS } (1 hr), } primary antibody overnight (1% BSA/TBS), secondary ( ultrasmall up } to 20nm } gold) for (2 hr), fix, embed and view. My previous standard } protocol was } including 5-10% normal serum in all the above solutions. } Has anyone else seen this increase in labelling frequency? } I would be interested in protocols that people are very pleased } with and give } consistant clean results. } Bottom line: Have any of you found any key tricks to getting the } immunogold } labelling of cryothin sections to perform without the "hitch". } } Thank you for any help! } } Robert Underwood } Sr. Research Scientist } U of Washington } } } ==============================Original } Headers============================== } 5, 20 -- From underwoo-at-u.washington.edu Thu Oct 13 10:24:40 2005 } 5, 20 -- Received: from mxout2.cac.washington.edu } (mxout2.cac.washington.edu [140.142.33.4]) } 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j9DFOdgw017788 } 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 13 Oct 2005 } 10:24:39 -0500 } 5, 20 -- Received: from hymn10.u.washington.edu } (hymn10.u.washington.edu [140.142.13.244]) } 5, 20 -- by mxout2.cac.washington.edu (8.13.4+UW05.04/8.13.4 } +UW05.09) with ESMTP id j9DFOciU013706 } 5, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA } bits=256 verify=NO) } 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 13 Oct 2005 } 08:24:38 -0700 } 5, 20 -- Received: from localhost (localhost [127.0.0.1]) } 5, 20 -- by hymn10.u.washington.edu (8.13.4+UW05.04/8.13.4 } +UW05.09) with ESMTP id j9DFOcs7031737 } 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 13 Oct 2005 } 08:24:38 -0700 } 5, 20 -- Received: from [140.142.8.53] by hymn10.u.washington.edu } via HTTP; Thu, 13 Oct 2005 08:24:38 PDT } 5, 20 -- Date: Thu, 13 Oct 2005 08:24:38 -0700 (PDT) } 5, 20 -- From: Robert A Underwood {underwoo-at-u.washington.edu} } 5, 20 -- To: Microscopy List {Microscopy-at-MSA.Microscopy.Com} } 5, 20 -- Subject: [Microscopy] Cryothin Immunogold? } 5, 20 -- Message-ID: {Pine.LNX. } 4.43.0510130824380.28728-at-hymn10.u.washington.edu} } 5, 20 -- MIME-Version: 1.0 } 5, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed } 5, 20 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__CT } 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, } __MIME_VERSION 0, __SANE_MSGID 0' } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 11, 20 -- From marc.pypaert-at-yale.edu Thu Oct 13 11:42:42 2005 11, 20 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu [130.132.232.48]) 11, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DGggJp020863 11, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 13 Oct 2005 11:42:42 -0500 11, 20 -- Received: from [130.132.234.111] (net234-111.med.yale.edu [130.132.234.111]) 11, 20 -- by biomed.med.yale.edu (PMDF V6.1-1 #30532) 11, 20 -- with ESMTP id {01LU5AZTFSKI002PRH-at-biomed.med.yale.edu} for 11, 20 -- Microscopy-at-MSA.Microscopy.Com; Thu, 13 Oct 2005 12:42:28 -0400 (EDT) 11, 20 -- Date: Thu, 13 Oct 2005 12:42:15 -0400 11, 20 -- From: Marc Pypaert {marc.pypaert-at-yale.edu} 11, 20 -- Subject: Re: [Microscopy] Cryothin Immunogold? 11, 20 -- In-reply-to: {200510131525.j9DFPK1k019461-at-ns.microscopy.com} 11, 20 -- To: underwoo-at-u.washington.edu 11, 20 -- Cc: Microscopy-at-MSA.Microscopy.Com 11, 20 -- Message-id: {0705CE0C-1210-43C8-8E97-83CF953444C5-at-yale.edu} 11, 20 -- MIME-version: 1.0 (Apple Message framework v728) 11, 20 -- X-Mailer: Apple Mail (2.728) 11, 20 -- Content-type: text/plain; format=flowed; delsp=yes; charset=US-ASCII 11, 20 -- Content-transfer-encoding: 7bit 11, 20 -- References: {200510131525.j9DFPK1k019461-at-ns.microscopy.com} ==============================End of - Headers==============================
I am sorry for the wrong links you might find if using the Internet-links given in my previous mail which also was sent out to the listserver.
Unfortunately you will be directed to a page with "German" text, stating that ALL LINKS of the RKI(Robert Koch Institute, Berlin have been subjected to link-address changes....(due to a new Content Management System)....
Unfortunately it seems there is no possibility to get the correct site(s) by means of pasting the URL's of the English-Version-documents
But perhaps you can try the following: } German { page, Find the link "ENGLISH" (left lower corner) and set "rapid EM" as the search phrase....perhaps then you will get linked with the pages I recommended in the mail before. At least I was able to retrieve the documents (esp. No 18....which really has the URL http://www.rki.de/cln_011/nn_231622/EN/Content/Institute/DepartmentsUnit s/NRC/CONSULAB/download__list__04.html__nnn=true ) that way again....
Regards Wolfgang
---------- Von: derby-at-nmt.edu[SMTP:derby-at-nmt.edu] Antwort an: derby-at-nmt.edu Gesendet: Donnerstag, 13. Oktober 2005 17:52 An: W.Muss-at-salk.at Betreff: [Microscopy] How to remove formalin
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Greetings to all,
I am a bit stumped. I have been asked to find a way to remove formalin, from formalin fixed viruses. Any tips/trick/methods would be appreciated.
Thank you,
Robert Derby New Mexico Tech.
==============================Original Headers============================== 5, 16 -- From derby-at-nmt.edu Thu Oct 13 10:46:56 2005 5, 16 -- Received: from mailhost.nmt.edu (mailhost.NMT.EDU [129.138.4.52]) 5, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DFku5v026743 5, 16 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 13 Oct 2005 10:46:56 -0500 5, 16 -- Received: from [129.138.14.28] (robert.nmt.edu [129.138.14.28]) 5, 16 -- by mailhost.nmt.edu (8.13.0/8.13.0) with ESMTP id j9DFkrEq014820 5, 16 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 13 Oct 2005 09:46:54 -0600 5, 16 -- User-Agent: Microsoft-Outlook-Express-Macintosh-Edition/5.02.2022 5, 16 -- Date: Thu, 13 Oct 2005 09:47:45 -0600 5, 16 -- Subject: How to remove formalin 5, 16 -- From: Robert {derby-at-nmt.edu} 5, 16 -- To: {Microscopy-at-msa.microscopy.com} 5, 16 -- Message-ID: {BF73DDC1.357%derby-at-nmt.edu} 5, 16 -- Mime-version: 1.0 5, 16 -- Content-type: text/plain; charset="US-ASCII" 5, 16 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 20, 28 -- From W.Muss-at-salk.at Thu Oct 13 11:45:04 2005 20, 28 -- Received: from Hermes.salk.at (hermes.lks.at [193.170.167.9]) 20, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DGj3p9025141 20, 28 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 13 Oct 2005 11:45:04 -0500 20, 28 -- Received: from localhost (localhost [127.0.0.1]) 20, 28 -- by hermes.lks.at (Postfix) with ESMTP id 9CBDD5A9031; 20, 28 -- Thu, 13 Oct 2005 18:45:02 +0200 (CEST) 20, 28 -- Received: from hermes.lks.at ([127.0.0.1]) 20, 28 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024) 20, 28 -- with ESMTP id 54906-06; Thu, 13 Oct 2005 18:45:02 +0200 (CEST) 20, 28 -- Received: from c1pa003 (unknown [192.168.42.3]) 20, 28 -- by hermes.lks.at (Postfix) with SMTP id 29AF35A902A; 20, 28 -- Thu, 13 Oct 2005 18:45:02 +0200 (CEST) 20, 28 -- Received: by localhost with Microsoft MAPI; Thu, 13 Oct 2005 18:44:56 +0200 20, 28 -- Message-ID: {01C5D026.34E22E20.W.Muss-at-salk.at} 20, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at} 20, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at} 20, 28 -- To: "'derby-at-nmt.edu'" {derby-at-nmt.edu} 20, 28 -- Cc: "'Microscopy-at-msa.microscopy.com'" {Microscopy-at-msa.microscopy.com} 20, 28 -- Subject: AW: [Microscopy] RE: apologies for poor links...How to remove formalin 20, 28 -- Date: Thu, 13 Oct 2005 18:44:55 +0200 20, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at} 20, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor 20, 28 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211 20, 28 -- MIME-Version: 1.0 20, 28 -- Content-Type: text/plain; charset="us-ascii" 20, 28 -- Content-Transfer-Encoding: 7bit 20, 28 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at ==============================End of - Headers==============================
This is not specifically a microscopy job, but it could be and there are members of the microscopy community with overlapping interests, so I thought that I would forward this to the listserver from Brian Holloway. Please do not contact me. -Scott
Here is their announcement: The Department of Applied Science at the College of William and Mary, an interdisciplinary PhD-focused department established in 1995, invites applicants for a tenure-track position at the assistant professor level in biophysics, neurophysiology, biomedical engineering, biomaterials, or a related field, emphasizing either computational or experimental approaches. The new faculty member will be expected to establish a vigorous, independent and wellfunded graduate research program at the interface of the physical, mathematical, and biological sciences. Excellence and high commitment to the teaching of graduate and undergraduate students is also expected of all faculty at the College. Located two hours south of Washington, D.C. in Williamsburg, Virginia, the College of William and Mary is the second-oldest university in the United States and it was recently names by the editors of Newsweek Magazine as the "hottest small state school" in the nation.
Candidates should submit a complete curriculum vitae, contact information for three letters of reference, and copies of no more than five refereed publications to: Faculty Search Committee, Department of Applied Science, The College of William & Mary, PO Box 8795, Williamsburg, VA 23187-8795. Review of materials is expected to begin January 1, 2006 and will continue until the position is filled.
The College is an EEO/AA employer. Chartered in 1693 by the King and Queen of England, William and Mary has approximately 5000 undergraduates and 2300 graduate and professional students in 19 advanced degree programs. Applied Science is an interdisciplinary graduate department that offers M.S. and Ph.D. degrees. In addition to the core faculty of the Department of Applied Science, faculty from the Departments of Biology, Chemistry, Computer Science, Mathematics, and Physics as well as from NASA's Langley Research Center, DoE's Jefferson Lab, and local industry participate under various levels of affiliation. http://www.as.wm.edu
-----Original Message----- X-from: Brian Holloway [mailto:holloway-at-AS.WM.EDU] Sent: Thursday, October 13, 2005 8:49 AM To: holloway-at-as.wm.edu
Shu-You Li
I believe that your intent maybe honest, however, the impact will be negative.
You would need a greater track-record before attempting to usurp this listserver.
regards,
JQuinn
} From mail-at-ns.microscopy.com Thu Oct 13 10:52:22 2005 } Date: Thu, 13 Oct 2005 09:56:30 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: syli-at-northwestern.edu } Reply-to: syli-at-northwestern.edu } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] Anew platform for microscopists to discuss microscopy online } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear list, } } I just created a new platform for us to discuss microscopy online using } Web Wiz Forums. It might be a better platform than listservers, especially } for those who want to reduce unnecessary emails. (I am not intending to } say this listserv is bad. In fact Nestor did a great job and the } listserv has served us for more than 10 years.) With the new online } forum you can still set email notification for the topics you are } interested in, at the same time you may ignore the topics out of } interest. } } Other features of this online discussion board include: } ***You need NOT register to post messages in most of the forums. However } you have to type in your name every time you post a message. Registered } users have full access to the website contents and functionality. } ***Students or any Microscopists may ask questions related to microscopy } in the Discussion board. } ***Employers may post vacant positions in the Jobs & Resumes. } ***Job-hunters are welcome to post their resumes too. } ***Users may post and discuss their recent publications in } recommended Readings. } ***Open facilities or EM consulting companies may post their contact } information in EM facilities nearby. } ***Software authors may post their beta tests or freewares/sharewares in } Software downloads. } ***Commercial companies may post their product information and contact } information free in EM companies } ***I am collecting Daily Operation Manuals for all kinds of microscopes. } Please send me your contributions to me. I will include your name as } courtesy. After I have collected sufficient amount of manuals I will put } them here so our users may benefit from your kindness. } ***Finally but not least, you may create your own customized interest } group in Customized Discussion Groups. You may set it as } access-by-authorization-only or open to everyone. } } The link to this forum is } http://www.ShuyouLi.com/ } } I welcome all kinds of comments, suggestions and criticisms - to make } us microscopists feel more convenient to discuss virtually world wide. } } Thanks, } Shuyou Li } } } _____________________________ } Shu-You Li, Ph.D. } Electron Microscopist, EPIC } NUANCE } Northwestern University } 2220 Campus Drive, 1161 Cook Hall (#2036 for mail) } Evanston, IL 60208-3108, USA } Ph: (847) 491-6723(O), (847) 491-7806(L), Fax: (847) 467-6573 } Email: syli-at-northwestern.edu; shuyouli-at-gmail.com } http://www.nuance.northwestern.edu/EPIC } http://www.shuyouli.com } } } } ==============================Original Headers============================== } 10, 16 -- From syli-at-northwestern.edu Thu Oct 13 09:55:41 2005 } 10, 16 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57]) } 10, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DEtfPA008880 } 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 09:55:41 -0500 } 10, 16 -- Received: from [129.105.37.16] (andromeda.ms.northwestern.edu [129.105.37.16]) } 10, 16 -- by merle.it.northwestern.edu (Postfix) with ESMTP id EE7109E847 } 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 09:55:40 -0500 (CDT) } 10, 16 -- Date: Thu, 13 Oct 2005 09:57:37 -0500 } 10, 16 -- From: Shu-You Li {syli-at-northwestern.edu} } 10, 16 -- To: microscopy-at-microscopy.com } 10, 16 -- Subject: Anew platform for microscopists to discuss microscopy online } 10, 16 -- Message-Id: {20051013095630.0382.SYLI-at-northwestern.edu} } 10, 16 -- MIME-Version: 1.0 } 10, 16 -- Content-Type: text/plain; charset="US-ASCII" } 10, 16 -- Content-Transfer-Encoding: 7bit } 10, 16 -- X-Mailer: Becky! ver. 2.20 [en] } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 12 -- From jquinn-at-www.matscieng.sunysb.edu Thu Oct 13 11:49:58 2005 7, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 7, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DGnvQx009074 7, 12 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 11:49:57 -0500 7, 12 -- Received: (from jquinn-at-localhost) 7, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id j9DGjhF08025; 7, 12 -- Thu, 13 Oct 2005 12:45:43 -0400 7, 12 -- Date: Thu, 13 Oct 2005 12:45:43 -0400 7, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 7, 12 -- Message-Id: {200510131645.j9DGjhF08025-at-www.matscieng.sunysb.edu} 7, 12 -- To: microscopy-at-microscopy.com, syli-at-northwestern.edu 7, 12 -- Subject: re: Anew Platform.......... ==============================End of - Headers==============================
I agree that I have much less track-record than Nestor or maybe anyone else on the list. And I will be more than happy to terminate this forum if MSA or another big name could have similiar bbs, or could I work together with Nestor to create an alternative. Anyway I am providing an option to listers.
Personally I will keep myself in this list for sure as long as we have posts here. And I will keep my eye open on the forum I created. If you think a web-based forum is better than email communications, I appreciate it. If no one is willing to go there, that is fine with me too.
BTW, Thanks for those who pointed out the broken link on registration page. I have corrected it.
Shuyou
----------------------- Original Message ----------------------- On Thu, 13 Oct 2005 12:45:43 -0400 Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} wrote: } } Shu-You Li } } I believe that your intent maybe honest, } however, the impact will be negative. } } You would need a greater track-record } before attempting to usurp this listserver. } } regards, } } JQuinn } } } From mail-at-ns.microscopy.com Thu Oct 13 10:52:22 2005 } } Date: Thu, 13 Oct 2005 09:56:30 -0500 } } To: jquinn-at-www.matscieng.sunysb.edu } } From: syli-at-northwestern.edu } } Reply-to: syli-at-northwestern.edu } } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } } Subject: [Microscopy] Anew platform for microscopists to discuss microscopy online } } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } } X-lewp: MicroscopyListSpam NAGS } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear list, } } } } I just created a new platform for us to discuss microscopy online using } } Web Wiz Forums. It might be a better platform than listservers, especially } } for those who want to reduce unnecessary emails. (I am not intending to } } say this listserv is bad. In fact Nestor did a great job and the } } listserv has served us for more than 10 years.) With the new online } } forum you can still set email notification for the topics you are } } interested in, at the same time you may ignore the topics out of } } interest. } } } } Other features of this online discussion board include: } } ***You need NOT register to post messages in most of the forums. However } } you have to type in your name every time you post a message. Registered } } users have full access to the website contents and functionality. } } ***Students or any Microscopists may ask questions related to microscopy } } in the Discussion board. } } ***Employers may post vacant positions in the Jobs & Resumes. } } ***Job-hunters are welcome to post their resumes too. } } ***Users may post and discuss their recent publications in } } recommended Readings. } } ***Open facilities or EM consulting companies may post their contact } } information in EM facilities nearby. } } ***Software authors may post their beta tests or freewares/sharewares in } } Software downloads. } } ***Commercial companies may post their product information and contact } } information free in EM companies } } ***I am collecting Daily Operation Manuals for all kinds of microscopes. } } Please send me your contributions to me. I will include your name as } } courtesy. After I have collected sufficient amount of manuals I will put } } them here so our users may benefit from your kindness. } } ***Finally but not least, you may create your own customized interest } } group in Customized Discussion Groups. You may set it as } } access-by-authorization-only or open to everyone. } } } } The link to this forum is } } http://www.ShuyouLi.com/ } } } } I welcome all kinds of comments, suggestions and criticisms - to make } } us microscopists feel more convenient to discuss virtually world wide. } } } } Thanks, } } Shuyou Li } } } } } } _____________________________ } } Shu-You Li, Ph.D. } } Electron Microscopist, EPIC } } NUANCE } } Northwestern University } } 2220 Campus Drive, 1161 Cook Hall (#2036 for mail) } } Evanston, IL 60208-3108, USA } } Ph: (847) 491-6723(O), (847) 491-7806(L), Fax: (847) 467-6573 } } Email: syli-at-northwestern.edu; shuyouli-at-gmail.com } } http://www.nuance.northwestern.edu/EPIC } } http://www.shuyouli.com } } } } } } } } ==============================Original Headers============================== } } 10, 16 -- From syli-at-northwestern.edu Thu Oct 13 09:55:41 2005 } } 10, 16 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57]) } } 10, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DEtfPA008880 } } 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 09:55:41 -0500 } } 10, 16 -- Received: from [129.105.37.16] (andromeda.ms.northwestern.edu [129.105.37.16]) } } 10, 16 -- by merle.it.northwestern.edu (Postfix) with ESMTP id EE7109E847 } } 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 09:55:40 -0500 (CDT) } } 10, 16 -- Date: Thu, 13 Oct 2005 09:57:37 -0500 } } 10, 16 -- From: Shu-You Li {syli-at-northwestern.edu} } } 10, 16 -- To: microscopy-at-microscopy.com } } 10, 16 -- Subject: Anew platform for microscopists to discuss microscopy online } } 10, 16 -- Message-Id: {20051013095630.0382.SYLI-at-northwestern.edu} } } 10, 16 -- MIME-Version: 1.0 } } 10, 16 -- Content-Type: text/plain; charset="US-ASCII" } } 10, 16 -- Content-Transfer-Encoding: 7bit } } 10, 16 -- X-Mailer: Becky! ver. 2.20 [en] } } ==============================End of - Headers============================== } }
Recently I have been examining precipitated silica with the TEM (80 KV) and have observed a complication. First I thought my scope was acting up (well it is a little) and giving me unstable magnification. I would examine a particle at one mag, go to a different magnification, maybe a third, come back to the original magnification and fins my particle has grown. It appeared to have lost detail too. Almost like popcorn expanding.
I spend a lot of time looking at calibration grids, changing magnification, spot size and stigmating to beat the band and convinced myself the scope was not changing magnification on me.
I prepped new samples, found a nice silica agglomerate and took a photo. I left the beam on in and took simply took photos every once and awhile ( about every 5-8 minutes) and my little fine grain agglomerate blew up like popcorn.
I suspect it's the chloroform I use to grind, ultrasonicate and disperse in. The silica hold trace levels of chloroform due to H-bonding until the beam cooks it out which causes the particle to expand, changing the shape and size of the particle.
So... Has anyone else had this experience and more importantly does anyone have a better method of prepping predicated silica for TEM analysis?
Thanks in advance!
Frank Karl Degussa Corporation Akron Technical Center 3500 Embassy Parkway Suite 100 Akron, Ohio 44333
330-668-2235 Ext. 238
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==============================Original Headers============================== 18, 17 -- From frank.karl-at-degussa.com Thu Oct 13 13:20:37 2005 18, 17 -- Received: from malmailout1.rz.itson.com (mailout1.degussa.com [193.100.56.173]) 18, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DIKaAE032602 18, 17 -- for {microscopy-at-msa.microscopy.com} ; Thu, 13 Oct 2005 13:20:37 -0500 18, 17 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [172.20.6.74]) 18, 17 -- by malmailout1.rz.itson.com (8.13.3/8.13.3/Debian-6) with ESMTP id j9DIBLaZ022863 18, 17 -- for {microscopy-at-msa.microscopy.com} ; Thu, 13 Oct 2005 20:11:56 +0200 18, 17 -- Subject: TEM and precipitated silica 18, 17 -- To: microscopy-at-msa.microscopy.com 18, 17 -- X-Mailer: Lotus Notes Release 6.5.2 June 01, 2004 18, 17 -- Message-ID: {OF8B5E6FE3.46D6ADA1-ON85257099.0063040C-85257099.0064B0BB-at-degussa.com} 18, 17 -- From: frank.karl-at-degussa.com 18, 17 -- Date: Thu, 13 Oct 2005 14:19:48 -0400 18, 17 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 18, 17 -- 10/13/2005 01:20:35 PM 18, 17 -- MIME-Version: 1.0 18, 17 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
The below reference describes the use of critical point drying for formalin removal from tissues for subsequent DNA extraction.
Formalin Removal from Archival Tissue by Critical Point Drying Sheng-Guo Fang, Qiu-Hong Wan, and Noboru Fujihara BioTechniques Vol. 33, No. 3: pp 604-611 (Sep 2002)
Best regards,
Angela
Angela V. Klaus, Ph.D. Director, Microscopy and Imaging Facility American Museum of Natural History Central Park West and 79th Street New York, NY 10024 USA
Tel: 212-769-5977 Email: avklaus-at-amnh.org
-----Original Message----- X-from: derby-at-nmt.edu [mailto:derby-at-nmt.edu] Sent: Thursday, October 13, 2005 11:47 AM To: avklaus-at-amnh.org
Greetings to all,
I am a bit stumped. I have been asked to find a way to remove formalin, from formalin fixed viruses. Any tips/trick/methods would be appreciated.
Thank you,
Robert Derby New Mexico Tech.
==============================Original Headers============================== 5, 16 -- From derby-at-nmt.edu Thu Oct 13 10:46:56 2005 5, 16 -- Received: from mailhost.nmt.edu (mailhost.NMT.EDU [129.138.4.52]) 5, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DFku5v026743 5, 16 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 13 Oct 2005 10:46:56 -0500 5, 16 -- Received: from [129.138.14.28] (robert.nmt.edu [129.138.14.28]) 5, 16 -- by mailhost.nmt.edu (8.13.0/8.13.0) with ESMTP id j9DFkrEq014820 5, 16 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 13 Oct 2005 09:46:54 -0600 5, 16 -- User-Agent: Microsoft-Outlook-Express-Macintosh-Edition/5.02.2022 5, 16 -- Date: Thu, 13 Oct 2005 09:47:45 -0600 5, 16 -- Subject: How to remove formalin 5, 16 -- From: Robert {derby-at-nmt.edu} 5, 16 -- To: {Microscopy-at-msa.microscopy.com} 5, 16 -- Message-ID: {BF73DDC1.357%derby-at-nmt.edu} 5, 16 -- Mime-version: 1.0 5, 16 -- Content-type: text/plain; charset="US-ASCII" 5, 16 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 17, 28 -- From avklaus-at-amnh.org Thu Oct 13 13:26:33 2005 17, 28 -- Received: from lepore.amnh.org (lepore.amnh.org [216.73.241.12]) 17, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DIQWEi008805 17, 28 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 13 Oct 2005 13:26:32 -0500 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by lepore.amnh.org (Postfix) with ESMTP id E3CD55B764; 17, 28 -- Thu, 13 Oct 2005 14:26:31 -0400 (EDT) 17, 28 -- Received: from lepore.amnh.org ([127.0.0.1]) 17, 28 -- by localhost (lepore.amnh.org [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id 20236-02; Thu, 13 Oct 2005 14:26:29 -0400 (EDT) 17, 28 -- Received: from Tomo2 (216-73-249-209.dynamic.amnh.org [216.73.249.209]) 17, 28 -- by lepore.amnh.org (Postfix) with ESMTP id 91A4C5B768; 17, 28 -- Thu, 13 Oct 2005 14:26:29 -0400 (EDT) 17, 28 -- From: "Angela Klaus" {avklaus-at-amnh.org} 17, 28 -- To: {derby-at-nmt.edu} 17, 28 -- Cc: {Microscopy-at-msa.microscopy.com} 17, 28 -- Subject: RE: [Microscopy] How to remove formalin 17, 28 -- Date: Thu, 13 Oct 2005 14:26:35 -0400 17, 28 -- MIME-Version: 1.0 17, 28 -- Content-Type: text/plain; 17, 28 -- charset="us-ascii" 17, 28 -- Content-Transfer-Encoding: 7bit 17, 28 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 17, 28 -- In-Reply-To: {200510131546.j9DFkxVU026812-at-ns.microscopy.com} 17, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 17, 28 -- Thread-Index: AcXQDVnRkXUB7MyQRsCNgHM88lCAYgAFfRDQ 17, 28 -- Message-Id: {20051013182629.91A4C5B768-at-lepore.amnh.org} 17, 28 -- X-Virus-Scanned: amavisd-new at amnh.org ==============================End of - Headers==============================
We need more information. What are you trying to accomplish? Do you want to study grain boundary using metallographic methods?
Stu Smalinskas, P.E. Metallurgist SKF USA Plymouth, Michigan
--- vincent.metzger-at-philips.com wrote:
} } Question: I would like to study the boundary grain } of Wfilament of lamp. I'm searching a way to prepare } the sample to make good observation of the grain } boundary.
Give me a managed, moderated listserver any day, I have no wish to sift through the illiterate, ignorant ramblings which are likely to dominate for the occasional speck of good information.
Remember all that stuff about that phony 'revolutionary' microscope -- what was it -- the Rolfe Royale, or somesuch?
"...........why has the scientific community suppressed this important invention?" etc, etc, etc.
What's this past tense doing in relation to Nestor, anyway?
Just an accidental grammatical imperfection, I hope.
cheers
rtch
Date sent: Thu, 13 Oct 2005 11:03:05 -0500 To: r.sims-at-auckland.ac.nz X-from: randerson20-at-tampabay.rr.com Send reply to: randerson20-at-tampabay.rr.com
I have received a couple of emails off list on this topic. Most of them are fairly pertinent. I appreciate veyr much.
When comparing BBS with email listservs, we must think collectively their pros and cons. An advantage of listserv is that we get posts in regular email inbox so we don't need to make special effort to visit BBS, and we are not getting too much emails from MSA. With the wonderful spam filtration by Nestor, we get 20-30 emails all relevant to mciroscopy everyday. It is not big deal as we receive hundreds of emails daily.
The shortage of listserv is classification and archiving. We ussually find it difficult to find information in old communications although Nestor has all emails archived from 1993.
Talking about the mixture of regular emails with listserv posts, it is advantageous but also can be shortcoming. I don't know how others do but I myself have to set a email filter to sort MSA emails to a certain folder and, if there is no very interesting topic, I will read these emails only when I have coffee break. It is no difference for me to go check a website or read special MSA email folder at leisure time. Yes we are getting not too much emails from MSA, but we should also consider those communications off list, as those I received today on this topic offline. Some of these communications are trully fruitful and worth accessible by others. With BBS we don't need worry spamming the "list".
I think the key issue here is whether the topic is time-sensitive. If the topics we are discussing need immediate response and we need never check back the topic after the discussion, then listserv is great. For the topics that are not time-sensitve, for example the pay-per-play EM serivices someone asked here the other day, it might be benificial to others who might need this information months later. In addition to information sharing, BBS is also good on file sharing, software programs, manuals, papers, product application notes, etc. All these kinds of information can be saved collectively on a easy to reach website.
Although I am keen to see a success of this website, I will face the truth and see how it goes in several months.
Thanks to all who have replied to this topic, Shuyou _____________________________ Shu-You Li, Ph.D. Electron Microscopist, EPIC NUANCE Northwestern University 2220 Campus Drive, 1161 Cook Hall (#2036 for mail) Evanston, IL 60208-3108, USA Ph: (847) 491-6723(O), (847) 491-7806(L), Fax: (847) 467-6573 Email: syli-at-northwestern.edu; shuyouli-at-gmail.com http://www.nuance.northwestern.edu/EPIC http://www.shuyouli.com
==============================Original Headers============================== 9, 16 -- From syli-at-northwestern.edu Thu Oct 13 14:35:48 2005 9, 16 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57]) 9, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DJZmjR002764 9, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 14:35:48 -0500 9, 16 -- Received: from [129.105.37.16] (andromeda.ms.northwestern.edu [129.105.37.16]) 9, 16 -- by merle.it.northwestern.edu (Postfix) with ESMTP id 949EF9E86D 9, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 14:35:48 -0500 (CDT) 9, 16 -- Date: Thu, 13 Oct 2005 14:37:44 -0500 9, 16 -- From: Shu-You Li {syli-at-northwestern.edu} 9, 16 -- To: microscopy-at-microscopy.com 9, 16 -- Subject: re: [Microscopy] re: Anew Platform.......... 9, 16 -- Message-Id: {20051013134545.0391.SYLI-at-northwestern.edu} 9, 16 -- MIME-Version: 1.0 9, 16 -- Content-Type: text/plain; charset="US-ASCII" 9, 16 -- Content-Transfer-Encoding: 7bit 9, 16 -- X-Mailer: Becky! ver. 2.20 [en] ==============================End of - Headers==============================
Does someone know of a gentle chemical etch that could be used on an ion milled sample of Al and Ni (not alloyed) to clean its surface (i.e., removal of ion mill contamination)? Ciao for now, Ken -- Kenneth JT Livi, Ph.D. Department of Earth and Planetary Sciences 3400 N. Charles St. Johns Hopkins University Baltimore, MD 21218 (410) 516-8342 (410) 516-7933 fax
==============================Original Headers============================== 1, 22 -- From klivi-at-jhu.edu Thu Oct 13 14:41:01 2005 1, 22 -- Received: from ipex3.johnshopkins.edu (ipex3.johnshopkins.edu [128.220.2.141]) 1, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DJf1wa011377 1, 22 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 14:41:01 -0500 1, 22 -- Received: from jhuml3.jhu.edu ([128.220.2.66]) 1, 22 -- by ipex3.johnshopkins.edu with ESMTP; 13 Oct 2005 15:41:01 -0400 1, 22 -- X-BrightmailFiltered: true 1, 22 -- X-Brightmail-Tracker: AAAAAA== 1, 22 -- X-IronPort-AV: i="3.97,211,1125892800"; 1, 22 -- d="scan'208"; a="119370245:sNHT18490340" 1, 22 -- Received: from [128.220.60.135] (Ipomoea.olin.jhu.edu [128.220.60.135]) 1, 22 -- by jhuml3.jhu.edu (PMDF V6.2-X20 #30840) 1, 22 -- with ESMTP id {0IOB009ZHDCCU2-at-jhuml3.jhu.edu} for microscopy-at-microscopy.com; 1, 22 -- Thu, 13 Oct 2005 15:41:01 -0400 (EDT) 1, 22 -- Date: Thu, 13 Oct 2005 15:40:59 -0400 1, 22 -- From: Kenneth Livi {klivi-at-jhu.edu} 1, 22 -- Subject: TEM Al-Ni foil cleaning 1, 22 -- X-Sender: klivi1-at-pop.jhu.edu 1, 22 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 1, 22 -- Message-id: {a06010209bf7467c0d9d8-at-[128.220.60.135]} 1, 22 -- MIME-version: 1.0 1, 22 -- Content-type: text/plain; format=flowed; charset=us-ascii ==============================End of - Headers==============================
Hi Shu-you, You seem to be copping a fair bit of flack for something which has obviously taken a lot of thought and work. I think you are to be congratulated and wish you luck, your site fills a real need, and augments rather than "replaces" this listserver.
The problem I see is that it will take a lot of continuing work to keep current. Many people have started, in a rush of enthusiasm, without really achieving the critical mass and longevity needed to become "part of the furniture" as are this listserver and the confocal equivalent. The University of Florida "tips and tricks" site http://www.biotech.ufl.edu/EM/tips/sem.html has the highest profile I know of, and I visit it. Yours is more ambitious, but if you can succeed you will give us all an important resource.
So all the best!
Sally
Dr SJ Stowe Facility Coordinator ANU Electron Microscopy Unit ANU CRICOS#00120C
} -----Original Message----- } From: syli-at-northwestern.edu [mailto:syli-at-northwestern.edu] } Sent: Friday, 14 October 2005 12:56 AM } To: sally.stowe-at-anu.edu.au } Subject: [Microscopy] Anew platform for microscopists to } discuss microscopy online } } } } } } --------------------------------------------------------------- } ------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
==============================Original Headers============================== 11, 27 -- From sally.stowe-at-anu.edu.au Thu Oct 13 18:47:14 2005 11, 27 -- Received: from mail.rsbs.anu.edu.au (mail.rsbs.anu.edu.au [150.203.72.70]) 11, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DNlDK4023025 11, 27 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 18:47:14 -0500 11, 27 -- Received: from rsbs2262 (rsbs2262.rsbs.anu.edu.au [150.203.36.144]) 11, 27 -- by mail.rsbs.anu.edu.au (Postfix) with ESMTP 11, 27 -- id 622DAF44248; Fri, 14 Oct 2005 09:46:55 +1000 (EST) 11, 27 -- Reply-To: {Sally.Stowe-at-anu.edu.au} 11, 27 -- From: "Sally Stowe" {Sally.Stowe-at-anu.edu.au} 11, 27 -- To: {syli-at-northwestern.edu} , {microscopy-at-microscopy.com} 11, 27 -- Subject: RE: [Microscopy] Anew platform for microscopists to discuss microscopy online 11, 27 -- Date: Fri, 14 Oct 2005 09:46:56 +1000 11, 27 -- Message-ID: {000101c5d050$6537bd40$9024cb96-at-rsbs.anu.edu.au} 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- charset="us-ascii" 11, 27 -- X-Priority: 3 (Normal) 11, 27 -- X-MSMail-Priority: Normal 11, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 11, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1506 11, 27 -- Importance: Normal 11, 27 -- In-Reply-To: {200510131455.j9DEtm4b009076-at-ns.microscopy.com} 11, 27 -- X-RSBS-MailScanner-Information: Please contact the ISP for more information 11, 27 -- X-RSBS-MailScanner: Found to be clean 11, 27 -- X-MailScanner-From: sally.stowe-at-anu.edu.au 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9DNlDK4023025 ==============================End of - Headers==============================
Thanks for the encouragement. I will keep this site in good shape with continuous efforts. If necessary I will of course invite volunteers to moderate the site together with me to keep fresh blood.
To avoid "splinter" answer-seekers and resources of the society, I put a sticky note in the discussion board to inform new comers try this listserv if they could not get answer from the website. In this way I hope the website can be a compensation to the listserv but not competition.
Some of you pointed out that I should have discussed this attempt BEFORE creating or annoucing the presence of the site. I do appologize if the website is totally unacceptable for some of the listers here.
With regards, Shuyou
----------------------- Original Message ----------------------- On Fri, 14 Oct 2005 09:46:56 +1000 "Sally Stowe" {Sally.Stowe-at-anu.edu.au} wrote: } Hi Shu-you, } You seem to be copping a fair bit of flack for something which has obviously } taken a lot of thought and work. I think you are to be congratulated and } wish you luck, your site fills a real need, and augments rather than } "replaces" this listserver. } } The problem I see is that it will take a lot of continuing work to keep } current. Many people have started, in a rush of enthusiasm, without really } achieving the critical mass and longevity needed to become "part of the } furniture" as are this listserver and the confocal equivalent. The } University of Florida "tips and tricks" site } http://www.biotech.ufl.edu/EM/tips/sem.html has the highest profile I know } of, and I visit it. Yours is more ambitious, but if you can succeed you } will give us all an important resource. } } So all the best! } } Sally } } } Dr SJ Stowe } Facility Coordinator } ANU Electron Microscopy Unit } ANU CRICOS#00120C } } } } } -----Original Message----- } } From: syli-at-northwestern.edu [mailto:syli-at-northwestern.edu] } } Sent: Friday, 14 October 2005 12:56 AM } } To: sally.stowe-at-anu.edu.au } } Subject: [Microscopy] Anew platform for microscopists to } } discuss microscopy online } } } } } } } } } } } } --------------------------------------------------------------- } } ------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy } } Society of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------- } } ------------- } } } } Dear list, } } } } I just created a new platform for us to discuss microscopy } } online using Web Wiz Forums. It might be a better platform } } than listservers, especially for those who want to reduce } } unnecessary emails. (I am not intending to say this listserv } } is bad. In fact Nestor did a great job and the listserv has } } served us for more than 10 years.) With the new online forum } } you can still set email notification for the topics you are } } interested in, at the same time you may ignore the topics out } } of interest. } } } } Other features of this online discussion board include: } } ***You need NOT register to post messages in most of the } } forums. However you have to type in your name every time you } } post a message. Registered users have full access to the } } website contents and functionality. ***Students or any } } Microscopists may ask questions related to microscopy in the } } Discussion board. ***Employers may post vacant positions in } } the Jobs & Resumes. ***Job-hunters are welcome to post their } } resumes too. ***Users may post and discuss their recent } } publications in recommended Readings. ***Open facilities or EM } } consulting companies may post their contact information in EM } } facilities nearby. ***Software authors may post their beta } } tests or freewares/sharewares in Software downloads. } } ***Commercial companies may post their product information and } } contact information free in EM companies ***I am collecting } } Daily Operation Manuals for all kinds of microscopes. Please } } send me your contributions to me. I will include your name as } } courtesy. After I have collected sufficient amount of manuals } } I will put them here so our users may benefit from your } } kindness. ***Finally but not least, you may create your own } } customized interest group in Customized Discussion Groups. You } } may set it as access-by-authorization-only or open to everyone. } } } } The link to this forum is } } http://www.ShuyouLi.com/ } } } } I welcome all kinds of comments, suggestions and criticisms - } } to make us microscopists feel more convenient to discuss } } virtually world wide. } } } } Thanks, } } Shuyou Li } } } } } } _____________________________ } } Shu-You Li, Ph.D. } } Electron Microscopist, EPIC } } NUANCE } } Northwestern University } } 2220 Campus Drive, 1161 Cook Hall (#2036 for mail) } } Evanston, IL 60208-3108, USA } } Ph: (847) 491-6723(O), (847) 491-7806(L), Fax: (847) 467-6573 } } Email: syli-at-northwestern.edu; shuyouli-at-gmail.com } } http://www.nuance.northwestern.edu/EPIC } } http://www.shuyouli.com } } } } } } } } ==============================Original } } Headers============================== } } 10, 16 -- From syli-at-northwestern.edu Thu Oct 13 09:55:41 2005 } } 10, 16 -- Received: from merle.it.northwestern.edu } } (merle.it.northwestern.edu [129.105.16.57]) } } 10, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with } } ESMTP id j9DEtfPA008880 } } 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct } } 2005 09:55:41 -0500 } } 10, 16 -- Received: from [129.105.37.16] } } (andromeda.ms.northwestern.edu [129.105.37.16]) } } 10, 16 -- by merle.it.northwestern.edu (Postfix) with } } ESMTP id EE7109E847 } } 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct } } 2005 09:55:40 -0500 (CDT) } } 10, 16 -- Date: Thu, 13 Oct 2005 09:57:37 -0500 } } 10, 16 -- From: Shu-You Li {syli-at-northwestern.edu} } } 10, 16 -- To: microscopy-at-microscopy.com } } 10, 16 -- Subject: Anew platform for microscopists to discuss } } microscopy online 10, 16 -- Message-Id: } } {20051013095630.0382.SYLI-at-northwestern.edu} } } 10, 16 -- MIME-Version: 1.0 } } 10, 16 -- Content-Type: text/plain; charset="US-ASCII" } } 10, 16 -- Content-Transfer-Encoding: 7bit } } 10, 16 -- X-Mailer: Becky! ver. 2.20 [en] } } ==============================End of - } } Headers============================== } }
This is nice but I don't think that it will stand up to the test of time.
Your GUI is good but the problem is that one has to wonder how long you will be around relative to Nestor. You are of a different venue than Nestor.
I'm betting on Nestor.
gary g.
At 07:57 AM 10/13/2005, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 10, 25 -- From gary-at-gaugler.com Thu Oct 13 19:49:22 2005 10, 25 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9E0nLRJ008275 10, 25 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 19:49:22 -0500 10, 25 -- Received: (qmail 7300 invoked from network); 13 Oct 2005 17:48:51 -0700 10, 25 -- Received: by simscan 1.1.0 ppid: 7284, pid: 7286, t: 3.8544s 10, 25 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1119 spam: 3.0.3 10, 25 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 25 -- by qsmtp3 with SMTP; 13 Oct 2005 17:48:48 -0700 10, 25 -- Message-Id: {6.2.3.4.2.20051013174341.02894d50-at-mail.calweb.com} 10, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 10, 25 -- Date: Thu, 13 Oct 2005 17:47:16 -0700 10, 25 -- To: syli-at-northwestern.edu 10, 25 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 25 -- Subject: Re: [Microscopy] Anew platform for microscopists to discuss 10, 25 -- microscopy online 10, 25 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 25 -- In-Reply-To: {200510131457.j9DEvcWG012187-at-ns.microscopy.com} 10, 25 -- References: {200510131457.j9DEvcWG012187-at-ns.microscopy.com} 10, 25 -- Mime-Version: 1.0 10, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 10, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp3.surewest.net 10, 25 -- X-Spam-Level: 10, 25 -- X-Spam-Status: No, score=-2.2 required=5.0 tests=AWL,BAYES_00 autolearn=ham 10, 25 -- version=3.0.3 ==============================End of - Headers==============================
If most of us agree that BBS is a better technology than listserv, I don't think we are lack of volunteers to maintain the forum. You are right "time can prove all".
Thanks for the comment, anyway. Shuyou
----------------------- Original Message ----------------------- On Thu, 13 Oct 2005 17:47:16 -0700 Gary Gaugler {gary-at-gaugler.com} wrote: } This is nice but I don't think that it will stand up } to the test of time. } } Your GUI is good but the problem is that one } has to wonder how long you will be around relative } to Nestor. You are of a different venue than Nestor. } } I'm betting on Nestor. } } gary g. } } } At 07:57 AM 10/13/2005, you wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear list, } } } } I just created a new platform for us to discuss microscopy online using } } Web Wiz Forums. It might be a better platform than listservers, especially } } for those who want to reduce unnecessary emails. (I am not intending to } } say this listserv is bad. In fact Nestor did a great job and the } } listserv has served us for more than 10 years.) With the new online } } forum you can still set email notification for the topics you are } } interested in, at the same time you may ignore the topics out of } } interest. } } } } Other features of this online discussion board include: } } ***You need NOT register to post messages in most of the forums. However } } you have to type in your name every time you post a message. Registered } } users have full access to the website contents and functionality. } } ***Students or any Microscopists may ask questions related to microscopy } } in the Discussion board. } } ***Employers may post vacant positions in the Jobs & Resumes. } } ***Job-hunters are welcome to post their resumes too. } } ***Users may post and discuss their recent publications in } } recommended Readings. } } ***Open facilities or EM consulting companies may post their contact } } information in EM facilities nearby. } } ***Software authors may post their beta tests or freewares/sharewares in } } Software downloads. } } ***Commercial companies may post their product information and contact } } information free in EM companies } } ***I am collecting Daily Operation Manuals for all kinds of microscopes. } } Please send me your contributions to me. I will include your name as } } courtesy. After I have collected sufficient amount of manuals I will put } } them here so our users may benefit from your kindness. } } ***Finally but not least, you may create your own customized interest } } group in Customized Discussion Groups. You may set it as } } access-by-authorization-only or open to everyone. } } } } The link to this forum is } } http://www.ShuyouLi.com/ } } } } I welcome all kinds of comments, suggestions and criticisms - to make } } us microscopists feel more convenient to discuss virtually world wide. } } } } Thanks, } } Shuyou Li } } } } } } _____________________________ } } Shu-You Li, Ph.D. } } Electron Microscopist, EPIC } } NUANCE } } Northwestern University } } 2220 Campus Drive, 1161 Cook Hall (#2036 for mail) } } Evanston, IL 60208-3108, USA } } Ph: (847) 491-6723(O), (847) 491-7806(L), Fax: (847) 467-6573 } } Email: syli-at-northwestern.edu; shuyouli-at-gmail.com } } http://www.nuance.northwestern.edu/EPIC } } http://www.shuyouli.com } } } } } } } } ==============================Original Headers============================== } } 10, 16 -- From syli-at-northwestern.edu Thu Oct 13 09:55:41 2005 } } 10, 16 -- Received: from merle.it.northwestern.edu } } (merle.it.northwestern.edu [129.105.16.57]) } } 10, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } } j9DEtfPA008880 } } 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 } } 09:55:41 -0500 } } 10, 16 -- Received: from [129.105.37.16] } } (andromeda.ms.northwestern.edu [129.105.37.16]) } } 10, 16 -- by merle.it.northwestern.edu (Postfix) with ESMTP id } } EE7109E847 } } 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 } } 09:55:40 -0500 (CDT) } } 10, 16 -- Date: Thu, 13 Oct 2005 09:57:37 -0500 } } 10, 16 -- From: Shu-You Li {syli-at-northwestern.edu} } } 10, 16 -- To: microscopy-at-microscopy.com } } 10, 16 -- Subject: Anew platform for microscopists to discuss } } microscopy online } } 10, 16 -- Message-Id: {20051013095630.0382.SYLI-at-northwestern.edu} } } 10, 16 -- MIME-Version: 1.0 } } 10, 16 -- Content-Type: text/plain; charset="US-ASCII" } } 10, 16 -- Content-Transfer-Encoding: 7bit } } 10, 16 -- X-Mailer: Becky! ver. 2.20 [en] } } ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (U.J.Potter) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, October 14, 2005 at 04:08:26 ---------------------------------------------------------------------------
Email: U.J.Potter Name: Ursula Potter
Organization: University of Bath
Title-Subject: [Filtered] Online Booking Software for EM & LM facilities
Question: Dear all,
I would appreciate any information or user comments from those who use online booking software. I am searching for a reasonably priced system for University-wide users that enables them to book Electron Microscope, Confocal and FACs equipment housed in a central unit. I have also come across a system called phpScheduleIt which seems to be freeware although I don't quite understand how this works - any comments on this would be very helpful.
I wanted to weigh in on this subject (please bear with me). As a preface: I've been a part of many forums, mostly automotive, a few other ones and would be an enthusiastic supporter of a UBB type forum, and also a long time Listserver subscriber (in various locations).
In fact its an idea that I've been letting roll around in my head for a few years - sort of hoping with all that incubation the idea might emerge as a pearl and take hold... no it hasn't emerged and I've been beaten to the punch. ;)
Problem is: we are a small (relatively) group, and a significant number of the microscopy community participates or subscribes to the list. Honestly, and maybe statistics will prove me wrong but it feels like the listserver is slightly decreasing in traffic. Maybe on par with the decrease in participation in local area meetings (shame on you all who don't make the effort to serve and participate in your LAS). There could be many causes. Or it is just my perception. And yes, like many I have a folder in my outlook that collects the listserver emails. I do tend to read all of them, or at least a significant number and I enjoy absorbing new information or perspectives or ways to explain aspects of what we all do.
But a forum style discussion group is different. Different in that you can have real-time posting/replies without the email going through a main server. It would cut down the times when you get replies before the email that you sent shows up in your inbox. It also can provide (depending on the forum set up) a format that allows easy posting of sample images, or diagrams. Often diagnosis is facilitated by images and a forum can provide a place to archive and share those images in a searchable database. On the flip side, if the forum succeeds it can disable a modest server relatively quickly. I've been involved in the growth of many forums. One now is the leading source for TDI information (tdiclub.com). It started as a small group and has grown immensely. It most likely far exceeds any numbers we could hope to attain as a microscopy forum. But the model is sound. Provide a place for experts and novices to have real time discussion, question and answer, with the ability to easily archive and search.
In the ideal world I think the forum should be set up with the support of good ol' Nestor and MSA. He's done so much to keep this listserver going and obviously has a lot invested in it, as do we all who participate. There are quite a few here who have been on longer than my 10 or so years (I think that's about right, between the different email accounts and all). And that is a core of email junkies. But about 6-7 years ago UBB came out with a program that changed listservers and newsgroups forever. There are many variations and I'm sure most everyone has seen/participated in one type of forum or another. The success of a forum is 100% dependent on the core users, and the collective participation/knowledge. I believe a microscopy forum could survive and do well and provide a valuable resource, and potentially could easily exist in tandem with the listserver, maybe even with MT or other publications.
Maybe Shuyou's forum is premature, maybe not. The data can easily be converted to different forum structures. It interface is easily modified and the archiving and searching is a breeze, depending on software and server structure.
Also, important to make note. A Forum can be made as secure and private (more so even) than a listserver. The administrator can grant access to view the discussion threads to only registered users. They can set it up to allow only registered users to post. They can also filter and restrict and monitor requests for usernames. All that without a huge amount of work. Okay there is a little bit of work, esp as the group gets larger. However, a forum of 38,000 registered users is well maintained by two administrators and a host of moderators. Typically one moderator per topic/discussion area, and it would probably be reasonably easy to agree upon one or two people with distinct experiences who could easily moderate the content in say an SEM or TEM forum. Very simple, very elegant.
My final words on this subject (for this email)... ;-) I'm of the opinion that listservers are a dinosaur. Kind of like 667 polaroid film. Yes, they still work to convey information, but: there are more modern methods to do the same thing, saving everyone on all ends much time and space. No more deleting emails you don't want to read - no more worring about finding the right topic, flame wars and arguments about who knows what (like this) can be sequestered or allowed to come to completion without filling everyone's inbox. Until there is a viable alternative up on the 'net, I will faithfully read and contribute to the Listserver in its current and future forms.
Just a few of my thoughts,
With deep respect for the Listserver and the members here, Geoff
Geoff Williams Leduc Bioimaging Facility Manager Brown University
Just a quick announcement inviting you all to attend SCANNING 2006, Tuesday, April 25 through Thursday, April 27 in Washington, D.C. The location is prime--just two doors down from The White House--and the meeting promises to be an excellent scientific forum. We encourage everyone to submit an abstract. THE DEADLINE FOR ABSTRACT SUBMISSION IS JANUARY 18, 2006.
A preliminary program is below for your review and we hope to see you there! Note the brand new course by Alan Boyde and new sessions being held by Ken Moore (Morphology Core), Elaine Humphrey (Univ. of BC), Robert Carlton (GlaxoSmithKline), Bev Giammara (Univ. of Louisville), Terry Allen (Christie Hospital NHS Trust), Warren MoberlyChan (Lawrence Livermore National Labs), Dale Newbury (NIST), Rob Apkarian (Emory Univ.), and Bruno Frohlich (Smithsonian Institution).
To download a more comprehensive Preliminary Program including summaries and the Registration Form or additional information, please visit www.scanning.org.
Among the Sessions and Courses SCANNING 2006—Washington, D.C., • April 25–27, 2006
Special New Course:
Skeletal Tissue Structural Biology and the Contribution and Potential of the Scanning Microscopies–NEW! Chair: Alan Boyde, Barts and the London School of Medicine and Dentistry and Queen Mary University of London, London, U.K.
The Role of Scanning Microscopies in the Study of Disease—NEW SESSION! Chair: Kenneth C. Moore, Morphology Core, Iowa City, Iowa, USA
Microwave in Microscopy–NEW SESSION! Chairs: Elaine Humphrey, University of British Columbia, Vancouver, BC and Beverly Giammara, University of Louisville School of Medicine, Louisville, Kentucky, USA
SEM of Biomaterials and Biomedical Devices–NEW SESSION! Chair: Robert Apkarian, Emory University, Atlanta, GA, USA
Applications of Environmental and Low Vacuum SEM in the Pharmaceutical Industry—NEW SESSION! Robert A. Carlton, GlaxoSmithKline, King of Prussia, PA, USA
Microscopies for the Structural and Dynamic Organization of the Nucleus—NEW SESSION! Chair: Terry D. Allen, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, U.K.
Forensic Science with a Special GSR Segment Chairs: S. Frank Platek, National Forensic Chemistry Center, U.S. Food and Drug Administration; M.A. Trimpe, Hamilton County Coroner’s Office, Cincinnati, OH, USA
Focused Ion Beam Microscopy Chair: Warren J. MoberlyChan, Materials Science and Technology Division, Lawrence Livermore National Laboratory, Livermore, CA, USA
Electron Beam/Specimen Interaction Workshop Chair: Dr. Michael T. Postek, Leader, Nano Scale Metrology, National Institute of Standards and Technology, Gaithersburg, MD, USA
The Electron Beam/Specimen Interaction Workshop has brought experimentalists and modeling experts together for nearly a decade to share information on this exciting topic.
Advances in Electron Beam Microanalysis of Individual Particles Chair: Dale E. Newbury NIST, Gaithersburg, MD, USA
Biological and Biomedical Applications of Scanning Microscopy Chair: Timothy Maugel, Laboratory for Biological Ultrastructure, Biology Department, University of Maryland, College Park, MD, USA
Scanning Museum Objects: Research, Documentation, and Preservation Chair: Bruno Frohlich, National Museum of Natural History, Smithsonian Institution, Washington, D.C., USA
Among the Short Courses:
Tuesday, April 25
Scanning Microscopy in Forensic Science Chairs: S. Frank Platek, National Forensic Chemistry Center, U.S. Food and Drug Administration, Cincinnati, OH; Michael T. Postek, US DOC-NIST, Gaithersburg, MD, M.A. Trimpe, Hamilton County Coroner’s Office, Cincinnati, OH, USA, and Michael McVicar, Chemistry Section Scientist, Center of Forensic Sciences, Ontario, Canada
Wednesday, April 26
Introduction to AFM—Sponsored by Pacific Nanotechnology, Inc. (Two-Day Course) Chair: Paul West, Pacific Nanotechnology, Inc., Tustin, CA, USA
Introduction to AFM (continued)—Sponsored by Pacific Nanotechnology, Inc. (Two-Day Course) Chair: Paul West, Pacific Nanotechnology, Inc., Tustin, CA, USA
Advanced Topics in SEM Chairs: D.C. Joy, Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN, USA; O. C. Wells, Yorktown Heights, NY, USA
Thursday, April 27
Mastering the Digital Image–NEW COURSE! Chair: J. Christian Russ, Reindeer Graphics, Asheville, NC, USA
Quantitative Measurements—Sponsored by Pacific Nanotechnology, Inc. (Half Day Course) Chair: Paul West, Pacific Nanotechnology, Inc., Tustin, CA, USA
Materials Sciences Applications—Sponsored by Pacific Nanotechnology, Inc. (Half Day Course) Chair: Paul West, Pacific Nanotechnology, Inc., Tustin, CA, USA
Dear collegues, You are invited to the Appalachian Regional Microscopy Society (AReMS) 2005 Fall Meeting at the Broyhill Inn and Conference Center, Boone, NC.
Theme: Nanoparticles in Biomedicine, Electronics & Material Science
Thursday, October 20, 2005
2:30-4:30 pm Workshop: Optical Microscopy at Sub-100nm Resolution, Rene Salazar, Aetos Technologies, Inc.
2:30-4:30 pm Workshop: Force Measurements and Pulling Using an Atomic Force Microscope, Keith Jones, Asylum Research
6:00-7:00 pm Social: Integon Room at the Broyhill Inn
7:00-8:00 pm Dinner: Bernhardt Room at the Broyhill Inn
8:00-9:00 pm Dinner talk by Keynote speaker: Dale Newbury, National Institute of Standards & Technology: “Not Just a Pretty Picture: X-ray Mapping is 50 Years Young, the Best is Yet to Come, and the Future is Now!”
Friday, October 21, 2004, Powers North (formerly Trillium North) Room
8:00-8:10 am Welcome, President Lou Germinario
8:15-8:45 am James Wittig, Vanderbilt University: “Metallic and Semiconducting Nanoparticles for Biomedical Applications”
8:50-9:20 am Jonathan Bender, University of South Carolina: “Nanotribology Study of Ultra-high Molecular Polyethylene”
9:25-9:55 am Dale Newbury, National Institute of Standards & Technology: “Blunders in Automatic Peak Identification of Major Constituents by Electron-excited Energy Dispersive X-ray Microanalysis”
10:15-10:30 am Introduction of Posters
10:35-11:05 am Richard Spontak, NC State University: “Direct Visualization of Polymer-Polymer Dewetting in the Presence of a Block Copolymer: From Macroscopic to Microscopic Mechanisms”
11:10-11:25 am Break and Posters Session
11:30-12:00 pm Sarah White, Hitachi High Technology: "Low Voltage STEM Imaging and Analysis of Catalysts, Nanoparticles, Nanomaterials, and Nanolayers"
For more information or to register online visit the website www.arems.org To register by phone call Sam Pennington at 704-825-8261 or by email at shpennington-at-alliedhightech.com
==============================Original Headers============================== 20, 20 -- From dale_batchelor-at-ncsu.edu Fri Oct 14 16:00:59 2005 20, 20 -- Received: from uni06mr.unity.ncsu.edu (uni06mr.unity.ncsu.edu [152.1.1.169]) 20, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9EL0t3U001040 20, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Fri, 14 Oct 2005 16:00:59 -0500 20, 20 -- Received: from [152.14.52.68] (dbmacx.aif.ncsu.edu [152.14.52.68]) 20, 20 -- by uni06mr.unity.ncsu.edu (8.13.4/8.13.4/N.20050816.01) with ESMTP id j9EL0nT6029259 20, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Fri, 14 Oct 2005 17:00:50 -0400 (EDT) 20, 20 -- Mime-Version: 1.0 (Apple Message framework v623) 20, 20 -- Message-Id: {2e079fb6ac3bb7ae4cd393dad627b972-at-ncsu.edu} 20, 20 -- Content-Type: text/plain; charset=WINDOWS-1252; format=flowed 20, 20 -- To: Microscopy-at-MSA.Microscopy.com 20, 20 -- From: Dale Batchelor {dale_batchelor-at-ncsu.edu} 20, 20 -- Subject: [Microscopy] AReMS 2005 Meeting 20, 20 -- Date: Fri, 14 Oct 2005 17:05:22 -0400 20, 20 -- X-Mailer: Apple Mail (2.623) 20, 20 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.0.3.2, Antispam-Data: 2005.10.14.25 20, 20 -- X-Spam-Status: No, Hits=7% 20, 20 -- X-Spam-Level: IIIIIII 20, 20 -- Content-Transfer-Encoding: 8bit 20, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9EL0t3U001040 ==============================End of - Headers==============================
One of our investigators is working on knockout mice whose intestinal villi are longer than those seen in wild type mice. She wants to show these by SEM and hopefully also measure the average height of the villi. The problem is how to prepare the intestine so that some of the villi are standing up and how to obtain preparations that will allow measurement of the villi.
I welcome any suggestions or references if you know of some older references.
Thanks,
Cora Bucana
==============================Original Headers============================== 6, 19 -- From bucana-at-audumla.mdacc.tmc.edu Fri Oct 14 16:44:14 2005 6, 19 -- Received: from mdairnmail2.mdacc.tmc.edu (mdairnmail2.mdacc.tmc.edu [143.111.251.124]) 6, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9ELiCSx010118 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2005 16:44:13 -0500 6, 19 -- Received: from ([143.111.64.70]) 6, 19 -- by mdairnmail2.mdacc.tmc.edu with ESMTP id KP-BRCRK.4799853; 6, 19 -- Fri, 14 Oct 2005 16:43:57 -0500 6, 19 -- Received: from cellbios56.audumla.mdacc.tmc.edu (cellbios56.mdacc.tmc.edu [143.111.150.56]) 6, 19 -- by audumla.mdacc.tmc.edu (8.12.10+Sun/8.12.10) with ESMTP id j9ELhvF5021032 6, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Oct 2005 16:43:57 -0500 (CDT) 6, 19 -- Message-Id: {5.1.1.6.0.20051014164014.01fa4de8-at-audumla.mdacc.tmc.edu} 6, 19 -- X-Sender: bucana-at-audumla.mdacc.tmc.edu 6, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 5.1.1 6, 19 -- Date: Fri, 14 Oct 2005 16:46:33 -0500 6, 19 -- To: Microscopy-at-microscopy.com 6, 19 -- From: "Corazon D. Bucana" {bucana-at-audumla.mdacc.tmc.edu} 6, 19 -- Subject: need help preparing intestinal villi for SEM and morphometry 6, 19 -- Mime-Version: 1.0 6, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Some of us are now receiving unsolicited private invitations from yahoo to join this new forum. It seems that behind Shuyou lurks our old friend Mr Sergey Ryazantsev complete with a considerable amount of political baggage in the invitation message with regard to Nestor and the current listserver. Therefore one needs to question the motives behind such an initiative, its stability, and its likely endurance into the future.
This list has been running very well almost since electricity was invented, thanks to Nestor, who for all we know may well have even invented electricity once. I'm staying with this platform, thanks.
==============================Original Headers============================== 3, 24 -- From ard-at-ansto.gov.au Fri Oct 14 18:08:54 2005 3, 24 -- Received: from tachyon.gw.ansto.gov.au (tachyon.gw.ansto.gov.au [137.157.8.253]) 3, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9EN8qjU019470 3, 24 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2005 18:08:53 -0500 3, 24 -- Received: (from uucp-at-localhost) 3, 24 -- by tachyon.gw.ansto.gov.au (8.11.7p1+Sun/8.11.7) id j9EN8pd13411 3, 24 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2005 09:08:51 +1000 (EST) 3, 24 -- Received: from jenner.ansto.gov.au(137.157.59.25) by tachyon.gw.ansto.gov.au via csmap (V6.0) 3, 24 -- id srcAAAXgaqmA; Sat, 15 Oct 05 09:08:51 +1000 3, 24 -- Received: from hadron.ansto.gov.au (hadron.ansto.gov.au [137.157.13.219]) 3, 24 -- by jenner.ansto.gov.au (8.12.10/8.12.10) with ESMTP id j9EN8otg021879 3, 24 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2005 09:08:50 +1000 3, 24 -- Received: from [137.157.95.82] (arthur.amat.ansto.gov.au [137.157.95.82]) 3, 24 -- by hadron.ansto.gov.au (8.9.3/8.9.3) with ESMTP id JAA00724 3, 24 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2005 09:08:48 +1000 (EST) 3, 24 -- Mime-Version: 1.0 3, 24 -- X-Sender: ard-at-hadron.ansto.gov.au 3, 24 -- Message-Id: {v04210104bf75e6a5b1df-at-[137.157.95.82]} 3, 24 -- Date: Sat, 15 Oct 2005 09:08:47 +1000 3, 24 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 3, 24 -- From: Arthur Day {ard-at-ansto.gov.au} 3, 24 -- Subject: Re: This new platform.... 3, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 24 -- X-ANSTO-MailScanner: Found to be clean ==============================End of - Headers==============================
Dear Cora, You could do TEM studies of it and measure the villus length, that's what my colleague did for starvation stress in mice, where the villus size changes. shashi CCMB Hyderabad INDIA --- bucana-at-audumla.mdacc.tmc.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi All, } } One of our investigators is working on knockout mice } whose intestinal villi } are longer than those seen in wild type mice. She } wants to show these by } SEM and hopefully also measure the average height of } the villi. The } problem is how to prepare the intestine so that some } of the villi are } standing up and how to obtain preparations that will } allow measurement of } the villi. } } I welcome any suggestions or references if you know } of some older references. } } Thanks, } } Cora Bucana } } } ==============================Original } Headers============================== } 6, 19 -- From bucana-at-audumla.mdacc.tmc.edu Fri Oct } 14 16:44:14 2005 } 6, 19 -- Received: from mdairnmail2.mdacc.tmc.edu } (mdairnmail2.mdacc.tmc.edu [143.111.251.124]) } 6, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with } ESMTP id j9ELiCSx010118 } 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 14 } Oct 2005 16:44:13 -0500 } 6, 19 -- Received: from ([143.111.64.70]) } 6, 19 -- by mdairnmail2.mdacc.tmc.edu with ESMTP } id KP-BRCRK.4799853; } 6, 19 -- Fri, 14 Oct 2005 16:43:57 -0500 } 6, 19 -- Received: from } cellbios56.audumla.mdacc.tmc.edu } (cellbios56.mdacc.tmc.edu [143.111.150.56]) } 6, 19 -- by audumla.mdacc.tmc.edu } (8.12.10+Sun/8.12.10) with ESMTP id j9ELhvF5021032 } 6, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 14 } Oct 2005 16:43:57 -0500 (CDT) } 6, 19 -- Message-Id: } {5.1.1.6.0.20051014164014.01fa4de8-at-audumla.mdacc.tmc.edu} } 6, 19 -- X-Sender: bucana-at-audumla.mdacc.tmc.edu } 6, 19 -- X-Mailer: QUALCOMM Windows Eudora Version } 5.1.1 } 6, 19 -- Date: Fri, 14 Oct 2005 16:46:33 -0500 } 6, 19 -- To: Microscopy-at-microscopy.com } 6, 19 -- From: "Corazon D. Bucana" } {bucana-at-audumla.mdacc.tmc.edu} } 6, 19 -- Subject: need help preparing intestinal } villi for SEM and morphometry } 6, 19 -- Mime-Version: 1.0 } 6, 19 -- Content-Type: text/plain; } charset="us-ascii"; format=flowed } ==============================End of - } Headers============================== }
__________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com
==============================Original Headers============================== 5, 20 -- From shashis_99-at-yahoo.com Fri Oct 14 23:45:59 2005 5, 20 -- Received: from web54615.mail.yahoo.com (web54615.mail.yahoo.com [206.190.49.185]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9F4jxfo031415 5, 20 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2005 23:45:59 -0500 5, 20 -- Received: (qmail 95676 invoked by uid 60001); 15 Oct 2005 04:45:59 -0000 5, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 20 -- s=s1024; d=yahoo.com; 5, 20 -- h=Message-ID:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 5, 20 -- b=E/fxN503O7grn9G0umOQ8iz/SjVukod9aySimXagyuQxUgh4SYqwAxQ7Wnp/fePRz4G0h4DS+V42ANq1WQWKolINymc6/NOWNLT+t01LspETfdQncyUsbHOL3/B8Pq3pehDotM/2NmGPH2HCHk79ig2rx9w1s8Y7BLftQQzM6a8= ; 5, 20 -- Message-ID: {20051015044558.95674.qmail-at-web54615.mail.yahoo.com} 5, 20 -- Received: from [203.200.217.180] by web54615.mail.yahoo.com via HTTP; Fri, 14 Oct 2005 21:45:58 PDT 5, 20 -- Date: Fri, 14 Oct 2005 21:45:58 -0700 (PDT) 5, 20 -- From: shashi singh {shashis_99-at-yahoo.com} 5, 20 -- Subject: Re: [Microscopy] need help preparing intestinal villi for SEM and morphometry 5, 20 -- To: bucana-at-audumla.mdacc.tmc.edu 5, 20 -- Cc: microscopy-at-microscopy.com 5, 20 -- In-Reply-To: {200510142145.j9ELjs02013190-at-ns.microscopy.com} 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; charset=iso-8859-1 5, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
The mission of the Interdisciplinary Center for Biotechnology Research (ICBR) is to support the growth of the life science research program of the University of Florida and that of researchers throughout the state, by making widely available the needed facilities, technologies, training, and competent personnel. ICBR is a service-oriented organization. The Director will manage the personnel and core facilities in support of the research faculty across all the colleges and departments of the University of Florida.
Interested candidates may contact me or go to this site and search the academic positions. http://jobs.ufl.edu/.
Gregory W. Erdos, Ph.D. Assistant Director, Interdisciplinary Center for Biotechnology Research Scientific Director, Electron Microscopy Core Lab P.O. Box 118525 University of Florida Gainesville, FL 32611 Phone: 352-392-1295 Fax: 352-846-0251 Email: gwe-at-ufl.edu
==============================Original Headers============================== 6, 20 -- From gwe-at-ufl.edu Sat Oct 15 09:15:50 2005 6, 20 -- Received: from smtp.ufl.edu (sp11en1.nerdc.ufl.edu [128.227.74.11]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9FEFo8I013215 6, 20 -- for {Microscopy-at-sparc5.microscopy.com} ; Sat, 15 Oct 2005 09:15:50 -0500 6, 20 -- Received: from uf-af2deb9258e4.ufl.edu (adsl-152-56-240.gnv.bellsouth.net [70.152.56.240]) 6, 20 -- (authenticated bits=0) 6, 20 -- by smtp.ufl.edu (8.13.1/8.13.1/2.5.0) with ESMTP id j9FEFlD8111280 6, 20 -- for {Microscopy-at-sparc5.microscopy.com} ; Sat, 15 Oct 2005 10:15:48 -0400 6, 20 -- Message-Id: {6.2.3.4.2.20051015101204.01e80338-at-biotech.ufl.edu} 6, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 6, 20 -- Date: Sat, 15 Oct 2005 10:15:55 -0400 6, 20 -- To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-ns.microscopy.com} 6, 20 -- From: Greg Erdos {gwe-at-ufl.edu} 6, 20 -- Subject: Biotech Director Job 6, 20 -- Mime-Version: 1.0 6, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 20 -- X-Spam-Status: hits=0.777, required=5, tests=BAYES_40,TO_ADDRESS_EQ_REAL 6, 20 -- X-UFL-Spam-Status: hits=0.777, required=5, tests=BAYES_40,TO_ADDRESS_EQ_REAL 6, 20 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 6, 20 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (winston.wiggins-at-cshs.org) from http://www.microscopy.com/MLFormMail.html on Friday, October 14, 2005 at 11:06:29 ---------------------------------------------------------------------------
Organization: Cedars-Sinai Medical Center, Los Angeles, CA
Title-Subject: [Filtered] MListserver: Nikon Small World Photomicrography competition
Question: Since 1974, Small World has assembled panels of scientists and photo editors to honor the best and brightest in the field of photomicrography,... Small World unabashedly celebrates the aesthetic side of what the microscope sees and the camera records... a chance for scientists to be celebrated as artists. "Scientists can face a long, arduous, technical road to scientific discovery," Eric Flem, who runs the Small World show, says. "But along the way, these guys see some absolutely beautiful images."
~ excerpt from "Mama Don't Take My Microscope" at http://www.wired.com/news/culture/0,1284,69191,00.html.
Competition is healthy and another source of help is always welcome. We'd all be still using candles if it wasnt for people like Edison coming up with alternatives for us to chose.
--- ard-at-ansto.gov.au wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Some of us are now receiving unsolicited private } invitations from } yahoo to join this new forum. It seems that behind } Shuyou lurks our } old friend Mr Sergey Ryazantsev complete with a } considerable amount } of political baggage in the invitation message with } regard to Nestor } and the current listserver. Therefore one needs to } question the } motives behind such an initiative, its stability, } and its likely } endurance into the future. } } This list has been running very well almost since } electricity was } invented, thanks to Nestor, who for all we know may } well have even } invented electricity once. I'm staying with this } platform, thanks. } } } ==============================Original } Headers============================== } 3, 24 -- From ard-at-ansto.gov.au Fri Oct 14 18:08:54 } 2005 } 3, 24 -- Received: from tachyon.gw.ansto.gov.au } (tachyon.gw.ansto.gov.au [137.157.8.253]) } 3, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with } ESMTP id j9EN8qjU019470 } 3, 24 -- for {microscopy-at-microscopy.com} ; Fri, 14 } Oct 2005 18:08:53 -0500 } 3, 24 -- Received: (from uucp-at-localhost) } 3, 24 -- by tachyon.gw.ansto.gov.au } (8.11.7p1+Sun/8.11.7) id j9EN8pd13411 } 3, 24 -- for {microscopy-at-microscopy.com} ; Sat, 15 } Oct 2005 09:08:51 +1000 (EST) } 3, 24 -- Received: from } jenner.ansto.gov.au(137.157.59.25) by } tachyon.gw.ansto.gov.au via csmap (V6.0) } 3, 24 -- id srcAAAXgaqmA; Sat, 15 Oct 05 09:08:51 } +1000 } 3, 24 -- Received: from hadron.ansto.gov.au } (hadron.ansto.gov.au [137.157.13.219]) } 3, 24 -- by jenner.ansto.gov.au (8.12.10/8.12.10) } with ESMTP id j9EN8otg021879 } 3, 24 -- for {microscopy-at-microscopy.com} ; Sat, 15 } Oct 2005 09:08:50 +1000 } 3, 24 -- Received: from [137.157.95.82] } (arthur.amat.ansto.gov.au [137.157.95.82]) } 3, 24 -- by hadron.ansto.gov.au (8.9.3/8.9.3) with } ESMTP id JAA00724 } 3, 24 -- for {microscopy-at-microscopy.com} ; Sat, 15 } Oct 2005 09:08:48 +1000 (EST) } 3, 24 -- Mime-Version: 1.0 } 3, 24 -- X-Sender: ard-at-hadron.ansto.gov.au } 3, 24 -- Message-Id: } {v04210104bf75e6a5b1df-at-[137.157.95.82]} } 3, 24 -- Date: Sat, 15 Oct 2005 09:08:47 +1000 } 3, 24 -- To: "Microscopy Listserver" } {microscopy-at-microscopy.com} } 3, 24 -- From: Arthur Day {ard-at-ansto.gov.au} } 3, 24 -- Subject: Re: This new platform.... } 3, 24 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } 3, 24 -- X-ANSTO-MailScanner: Found to be clean } ==============================End of - } Headers============================== }
==============================Original Headers============================== 4, 19 -- From s2kdude-at-pacbell.net Sat Oct 15 13:20:48 2005 4, 19 -- Received: from web80727.mail.yahoo.com (web80727.mail.yahoo.com [66.163.170.92]) 4, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9FIKmxV032482 4, 19 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2005 13:20:48 -0500 4, 19 -- Received: (qmail 8711 invoked by uid 60001); 15 Oct 2005 18:20:47 -0000 4, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 4, 19 -- s=s1024; d=pacbell.net; 4, 19 -- h=Message-ID:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 4, 19 -- b=jrg+c+YFzaVseKHyqSAM71EDzU3/dEX6u/pfLM33RpaprXJcpQLcHLBvmp9Zb1FgSXjl26j2z/fmmN8g98J6hZfuUVqprjy7yBUw2Ga4ltMHOEFjTFOdRj6AHpm+aTzb5IkZcXFKQ/PLYqLhhczyQYUgkhfAXPfnjmfE38K/H5w= ; 4, 19 -- Message-ID: {20051015182047.8709.qmail-at-web80727.mail.yahoo.com} 4, 19 -- Received: from [69.226.105.75] by web80727.mail.yahoo.com via HTTP; Sat, 15 Oct 2005 11:20:47 PDT 4, 19 -- Date: Sat, 15 Oct 2005 11:20:47 -0700 (PDT) 4, 19 -- From: "r. williams" {s2kdude-at-pacbell.net} 4, 19 -- Subject: Re: [Microscopy] Re: This new platform.... 4, 19 -- To: microscopy-at-microscopy.com 4, 19 -- In-Reply-To: {200510142314.j9ENEeKw026600-at-ns.microscopy.com} 4, 19 -- MIME-Version: 1.0 4, 19 -- Content-Type: text/plain; charset=iso-8859-1 4, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
A few additional points about the Microscopy Listserver. * I would hate to have to go looking for info, or have to specify topics of interest (it's all potentially interesting). Given my other obligations, I would be much less likely to seek out the info, but I'm happy to have it thrust in front of me. When I'm too busy, I simply ignore the list message that day. * I enjoy opening my email to all sorts of interesting questions, answers, and discussion about various aspects of microscopy. It's all right there. Sure, much of the discussion is not of interest to me at the moment, but I learn lots by perusing the emails, even on topics I would not have thought of as being interesting. * The point has already been made that it's not such a large traffic volume as to be unmanageable, and it's easy enough to hit the delete button based on the message subject line. One could even set the junk mail filter to automatically identify the list messages, then un-junk the ones of interest, and auto-delete all the rest with one click. * In other words, there are some real advantages to the email listserve format. If there are real needs for additional capabilities, such as a place to post image files that are referenced in the emails, perhaps Nestor could take with up with MSA. * Finally, I will add my "thank you!" to Nestor for maintaining such a useful list as a public service, in spite of the occassional complaint and with little thanks (I'm sure). --Jan Factor
--------------------------------------- Jan Robert Factor, Ph.D. Professor of Biology --------------------------------------- Natural Sciences Purchase College State University of New York 735 Anderson Hill Rd. Purchase, NY 10577 USA --------------------------------------- Office Tel: 914-251-6659 Office Fax: 914-251-6635 E-mail: jfactor-at-ns.purchase.edu or- jan.factor-at-purchase.edu ---------------------------------------
==============================Original Headers============================== 2, 23 -- From jfactor-at-ns.purchase.edu Sat Oct 15 13:58:20 2005 2, 23 -- Received: from mta5.srv.hcvlny.cv.net (mta5.srv.hcvlny.cv.net [167.206.4.200]) 2, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9FIwJUu008963 2, 23 -- for {microscopy-at-msa.microscopy.com} ; Sat, 15 Oct 2005 13:58:19 -0500 2, 23 -- Received: from [67.87.230.14] (ool-4357e60e.dyn.optonline.net [67.87.230.14]) 2, 23 -- by mta5.srv.hcvlny.cv.net 2, 23 -- (Sun Java System Messaging Server 6.2-2.06 (built May 11 2005)) 2, 23 -- with ESMTP id {0IOF00M0S0P67AJ2-at-mta5.srv.hcvlny.cv.net} for 2, 23 -- microscopy-at-msa.microscopy.com; Sat, 15 Oct 2005 14:58:19 -0400 (EDT) 2, 23 -- Date: Sat, 15 Oct 2005 14:58:24 -0400 2, 23 -- From: Jan Factor {jfactor-at-ns.purchase.edu} 2, 23 -- Subject: Re: [Microscopy] RE: Anew platform for microscopists to discuss 2, 23 -- microscopy online 2, 23 -- In-reply-to: {200510132348.j9DNmSUv024649-at-ns.microscopy.com} 2, 23 -- To: Microscopy Listserver {microscopy-at-msa.microscopy.com} 2, 23 -- Message-id: {43515150.1060801-at-ns.purchase.edu} 2, 23 -- MIME-version: 1.0 2, 23 -- Content-type: text/plain; charset=us-ascii; format=flowed 2, 23 -- Content-transfer-encoding: 7BIT 2, 23 -- X-Accept-Language: en-us, en 2, 23 -- References: {200510132348.j9DNmSUv024649-at-ns.microscopy.com} 2, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 2, 23 -- Gecko/20040804 Netscape/7.2 (ax) ==============================End of - Headers==============================
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==============================Original Headers============================== 6, 20 -- From opto-at-klughammer.de Sat Oct 15 14:21:31 2005 6, 20 -- Received: from moutng.kundenserver.de (moutng.kundenserver.de [212.227.126.177]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9FJLVPs017718 6, 20 -- for {microscopy-at-msa.microscopy.com} ; Sat, 15 Oct 2005 14:21:31 -0500 6, 20 -- Received: from p549751C1.dip.t-dialin.net [84.151.81.193] (helo=[192.168.2.100]) 6, 20 -- by mrelayeu.kundenserver.de with ESMTP (Nemesis), 6, 20 -- id 0ML29c-1EQraz1m55-0001La; Sat, 15 Oct 2005 21:21:29 +0200 6, 20 -- Message-ID: {435156B2.4060403-at-klughammer.de} 6, 20 -- Date: Sat, 15 Oct 2005 21:21:22 +0200 6, 20 -- From: Klughammer GmbH {opto-at-klughammer.de} 6, 20 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 6, 20 -- X-Accept-Language: de-DE, de, en-us, en 6, 20 -- MIME-Version: 1.0 6, 20 -- To: microscopy-at-msa.microscopy.com 6, 20 -- Subject: Re: [Microscopy] Anew platform for microscopists to discuss 6, 20 -- References: {200510151900.j9FJ0CqX013025-at-ns.microscopy.com} 6, 20 -- In-Reply-To: {200510151900.j9FJ0CqX013025-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- X-Provags-ID: kundenserver.de abuse-at-kundenserver.de login:0ff4ff792c9129f92cf390aadeab6682 ==============================End of - Headers==============================
Add my voice to this chorus. I agree that this format allows me the extra pleasure of serendipity as well as choice.
Joel
Date sent: Sat, 15 Oct 2005 14:21:39 -0500 To: jbs-at-temple.edu X-from: opto-at-klughammer.de Send reply to: opto-at-klughammer.de
Sure, but how come you always light the candles when the electricity fails.......
Cheers, Per
Per Horstedt Inst of medical biosciences Pathology Dept of electron microscopy University of Umea S-90185 Umea Sweden
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==============================Original Headers============================== 16, 22 -- From per.horstedt-at-medbio.umu.se Sat Oct 15 18:53:33 2005 16, 22 -- Received: from av9-2-sn3.vrr.skanova.net (av9-2-sn3.vrr.skanova.net [81.228.9.186]) 16, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9FNrXlw005061 16, 22 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2005 18:53:33 -0500 16, 22 -- Received: by av9-2-sn3.vrr.skanova.net (Postfix, from userid 502) 16, 22 -- id 7288A37F62; Sun, 16 Oct 2005 01:53:32 +0200 (CEST) 16, 22 -- Received: from smtp3-1-sn3.vrr.skanova.net (smtp3-1-sn3.vrr.skanova.net [81.228.9.101]) 16, 22 -- by av9-2-sn3.vrr.skanova.net (Postfix) with ESMTP id 582BE37F39 16, 22 -- for {microscopy-at-microscopy.com} ; Sun, 16 Oct 2005 01:53:32 +0200 (CEST) 16, 22 -- Received: from perhot3.medbio.umu.se (h133n1fls32o1117.telia.com [213.65.58.133]) 16, 22 -- by smtp3-1-sn3.vrr.skanova.net (Postfix) with ESMTP id 1EA0137E45 16, 22 -- for {microscopy-at-microscopy.com} ; Sun, 16 Oct 2005 01:53:31 +0200 (CEST) 16, 22 -- Message-Id: {6.2.3.4.2.20051016014756.01db74a0-at-medbio.umu.se} 16, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 16, 22 -- Date: Sun, 16 Oct 2005 01:53:33 +0200 16, 22 -- To: microscopy-at-microscopy.com 16, 22 -- From: Per =?iso-8859-1?Q?H=F6rstedt?= {per.horstedt-at-medbio.umu.se} 16, 22 -- Subject: Re: [Microscopy] This new platform.... 16, 22 -- In-Reply-To: {200510151821.j9FILGvt000534-at-ns.microscopy.com} 16, 22 -- References: {200510151821.j9FILGvt000534-at-ns.microscopy.com} 16, 22 -- Mime-Version: 1.0 16, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (loz_jay01-at-hotmail.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, October 16, 2005 at 06:59:58 ---------------------------------------------------------------------------
Email: loz_jay01-at-hotmail.com Name: laura
Organization: richard lander school
Education: 9-12th Grade High School
Location: truro,cornwall
Question: please could you tell me what procedure scientists use to look at cancer cells under the microscope
I am using a particular software for determining particle (and included grains) identification. For separating touching particles the software offers parameterization of 3 criteria: 'F', for control with regard to features across the surface (e.g., cracks); 'T', for controlling particles touching at a point; and 'E', for some control of particles touching along longer joins. What makes the parameters a bit difficult to experiment with is the number of permutations ... That is, the are 3 parameters for 'F' and 3 for 'E', for 7 total.
Does anyone recognize these parameters, such that they may provide me with a reference for understanding parameter values and how they may affect one another?
TIA ... michael shaffer :o) Memorial University St. John's Newfoundland
==============================Original Headers============================== 4, 19 -- From michael-at-shaffer.net Sun Oct 16 12:31:13 2005 4, 19 -- Received: from ws6-1.us4.outblaze.com (ws6-1.us4.outblaze.com [205.158.62.196]) 4, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9GHVBBF025805 4, 19 -- for {Microscopy-at-microscopy.com} ; Sun, 16 Oct 2005 12:31:13 -0500 4, 19 -- Received: (qmail 28110 invoked from network); 16 Oct 2005 17:31:10 -0000 4, 19 -- Received: from unknown (HELO rarewolf1) (michael-at-shaffer.net-at-205.251.84.119) 4, 19 -- by ws6-1.us4.outblaze.com with SMTP; 16 Oct 2005 17:31:10 -0000 4, 19 -- From: "michael shaffer" {michael-at-shaffer.net} 4, 19 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com} 4, 19 -- Subject: Image analysis - separating touching particles 4, 19 -- Date: Sun, 16 Oct 2005 15:01:04 -0230 4, 19 -- Message-ID: {000e01c5d277$63c12270$4f01a8c0-at-rarewolf1} 4, 19 -- MIME-Version: 1.0 4, 19 -- Content-Type: text/plain; 4, 19 -- charset="US-ASCII" 4, 19 -- Content-Transfer-Encoding: 7bit 4, 19 -- X-Mailer: Microsoft Office Outlook 11 4, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 4, 19 -- Thread-Index: AcXSd2Jha6R4ryCNQ9C0R/1CmPLu5Q== ==============================End of - Headers==============================
can you just clarify whether it is the villi you are trying to measure or the microvilli, because villi would be better done by some form of light microscopy.
Thanks Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences University of Sunderland Tyne & Wear SR1 3SD UK
e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: shashis_99-at-yahoo.com
Dear Ursala
I had a look at phpScheduleIt previously but it is Unix based and apparently needs an Apache web server ('Is that a helicopter dear?' - we're out of them at the moment anyway).
I did think of doing something clever with Access, but ultimately we do fine with separate A4 sheets, produced in word, one page per week, one month in advance. The sheets are located outside the microscope rooms in a wall-mounted clear plastic A4 display folders (with a pencil and a rubber). It works better than a diary at least, and 'this week' only is 'on view'. It also means you don't need a network linked laptop to see if the microscope is free, and the 'hard copies' are easy to read. However if anyone has any facility management software that's virtually free (and not £500 like most on the web), I would be interested (possibly).
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
----- Original Message ----- X-from: {U.J.Potter-at-ns.microscopy.com} To: {keith.morris-at-ucl.ac.uk} Sent: Friday, October 14, 2005 1:48 PM
Available - complete Balzers 400D Freeze Fracture System with:
- electron beam guns EK552 for Pt-C and C, - quartz crystal thin film monitor QSD-201D - freeze etching unit control BMS101, - HT unit control EVM052, - pumping unit control DPA101, - power distributor BNV201 - rotary pump (Pfeiffer DUO030A) - liq. nitrogen supply FET003 - threefold specimen table for cleaving - double replica specimen table for simultaneous breaking up of 3 sandwich carrier
Also included are - extra HT unit control EVM052, - extra freeze etching unit control GA-1, - extra quartz crystal thin film monitor QSG101
Must move immediately for best offer.
Have a view of the device at http://www.mardre.com/homepage/mic/tem/equipment/baf400.html
******************************************* Dr. Markus Drechsler Electronmicroscopy SFB481/BIMF/BZKG University of Bayreuth, NW2 Universitaetsstr. 30 D-95440 Bayreuth Tel: +49 (0)921 55-3188 Fax: +49 (0)921 55-3116
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (oxfordmaterials-at-hotmail.com) from http://microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, October 17, 2005 at 05:39:54 ---------------------------------------------------------------------------
Email: oxfordmaterials-at-hotmail.com Name: Mike Dowling
Organization: Department of Materials, Oxford University
Education: Undergraduate College
Location: Oxford, UK
Question: I am currently heading a third-year undergraduate Team Design Project at the Materials Department of Oxford University; our aim being to design a hypothetical SEM stage and environmental cell for in-situ CVD synthesis and observation of carbon nanotubes.
We would intend to grow the tubes in a low pressure (approx 1.5 Torr) methane environment, between 700 and 1100C. This hypothetical design currently includes a water-cooled heating stage equipped with screens to contain thermally emitted electrons, which would hopefully also contain the gases sufficiently, and negate the need for an expensive environmental cell unit.
We believe that this technology would be useful for studying nanotubes, but could also be adapted to form a stage that would fit inside any existing SEM for a variety of different high temperature dynamic reactions.
We would be very grateful to recieve any help, ideas or advice that you may have at oxfordmaterials-at-hotmail.com.
Thank you very much for your time,
Mike Dowling et al. Department of Materials, Oxford University.
A very good online scheduling system developed and maintained by the Complex Carbohydrate Research Center at The University of Georgia is offered free to users. The URL is http://faces.ccrc.uga.edu/. You can click on the 'Join Us' or 'FAQs' for more information.
Regards,
S. Kelly Sears
keith.morris-at-ucl.ac.uk wrote:
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==============================Original Headers============================== 9, 34 -- From sksears-at-eps.mcgill.ca Mon Oct 17 09:12:28 2005 9, 34 -- Received: from drizzle.cc.mcgill.ca (drizzle.CC.McGill.CA [132.206.27.48]) 9, 34 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9HECQUK019456 9, 34 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2005 09:12:27 -0500 9, 34 -- Received: from mailscan5.CC.McGill.CA (mailscan5.CC.McGill.CA [132.216.77.252]) 9, 34 -- by drizzle.cc.mcgill.ca (8.12.11/8.12.3) with ESMTP id j9HECLYp016319 9, 34 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2005 10:12:22 -0400 9, 34 -- Received: from cobbles.eps.mcgill.ca (cobbles.eps.mcgill.ca [132.206.152.2]) 9, 34 -- by mailscan5.CC.McGill.CA (8.12.11/8.12.11) with ESMTP id j9HE8Iqn003971 9, 34 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2005 10:08:19 -0400 9, 34 -- Received: by cobbles.eps.mcgill.ca (Postfix, from userid 508) 9, 34 -- id AD045231CC4; Mon, 17 Oct 2005 10:12:01 -0400 (EDT) 9, 34 -- Received: from eps.mcgill.ca (dhcp21643012.Medicine.McGill.CA [132.216.43.12]) 9, 34 -- by cobbles.eps.mcgill.ca (Postfix) with ESMTP id 1B509231CC3 9, 34 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2005 10:12:00 -0400 (EDT) 9, 34 -- Message-ID: {4353B177.4030409-at-eps.mcgill.ca} 9, 34 -- Date: Mon, 17 Oct 2005 10:13:11 -0400 9, 34 -- From: "S. Kelly Sears" {sksears-at-eps.mcgill.ca} 9, 34 -- Organization: Facility for Electron Microscopy Research 9, 34 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 9, 34 -- X-Accept-Language: en-us, en 9, 34 -- To: microscopy-at-microscopy.com 9, 34 -- Subject: Re: online booking software 9, 34 -- References: {200510170942.j9H9gdGv025443-at-ns.microscopy.com} 9, 34 -- In-Reply-To: {200510170942.j9H9gdGv025443-at-ns.microscopy.com} 9, 34 -- X-Spam-Checker-Version: SpamAssassin 2.60 (1.212-2003-09-23-exp) on 9, 34 -- cobbles.eps.mcgill.ca 9, 34 -- X-Spam-Level: 9, 34 -- X-Spam-Status: No, hits=-102.5 required=5.0 tests=BAYES_00,BUGGY_CGI, 9, 34 -- USER_IN_WHITELIST autolearn=no version=2.60 9, 34 -- X-Sanitizer: Advosys mail filter 9, 34 -- MIME-Version: 1.0 9, 34 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 9, 34 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Two postdoctoral research associate positions are now opened is the group of material science within the Center of Electron Microscopy of Ulm University (Germany).
Positions are related to research projects devoted to the study of magnetic nanomaterials and nanoporous oxides. The main research instrument for these studies will be Titan 80-300 (HAADF STEM detector, imaging Cs-corrector, Tridiem GIF, biprizm and Lorenz lens), which is currently under installation in Ulm uni. There is a number of other instruments in the Center, including CM20 TEM with heating and cooling holders and Hitachi S5200 high resolution SEM.
Deadline for applications is 21st of November 2005. Details can be found at http://www.uni-ulm.de/elektronenmikroskopie/mattem following the link "Vacancies".
_________________ Andrey Chuvilin, PhD University Ulm Zentrale Einrichtung Elektronenmikroskopie Materialwissenschaftliche Elektronenmikroskopie Albert Einstein Allee 11 D-89069 ULM Tel.: ++49-731-50-22941 Fax.: ++49-731-50-22958 Email: andrey.chuvilin-at-uni-ulm.de http://www.uni-ulm.de/elektronenmikroskopie/mattem/
==============================Original Headers============================== 6, 24 -- From a.chuvilin-at-microscopist.ru Mon Oct 17 09:27:46 2005 6, 24 -- Received: from mail.uni-ulm.de (mail.uni-ulm.de [134.60.1.1]) 6, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9HERjxl028156 6, 24 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2005 09:27:46 -0500 6, 24 -- Received: from obsidian (obsidian.physik.uni-ulm.de [134.60.11.27]) 6, 24 -- by mail.uni-ulm.de (8.13.4/8.13.4) with ESMTP id j9HERiOQ028301 6, 24 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2005 16:27:45 +0200 (MEST) 6, 24 -- Message-ID: {008d01c5d326$e332c360$1b0b3c86-at-obsidian} 6, 24 -- Reply-To: "Dusha" {a.chuvilin-at-microscopist.ru} 6, 24 -- From: "Dusha" {a.chuvilin-at-microscopist.ru} 6, 24 -- To: {microscopy-at-microscopy.com} 6, 24 -- Subject: TEM Postdoc positions 6, 24 -- Date: Mon, 17 Oct 2005 16:27:17 +0200 6, 24 -- MIME-Version: 1.0 6, 24 -- Content-Type: text/plain; 6, 24 -- format=flowed; 6, 24 -- charset="windows-1251"; 6, 24 -- reply-type=response 6, 24 -- Content-Transfer-Encoding: 7bit 6, 24 -- X-Priority: 3 6, 24 -- X-MSMail-Priority: Normal 6, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2527 6, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2527 6, 24 -- X-DCC-meer-Metrics: gemini 1086; Body=2 Fuz1=2 Fuz2=2 ==============================End of - Headers==============================
The AMOLF FOM Institute for Atomic and Moleculair physics (Amsterdam, The Netherlands) has purchased one only recently. You could contact Jaap Boon for more information J.Boon-at-amolf.nl. However, the AMOLF is not a service lab!
Ineke Joosten
onderzoeker afdeling Onderzoek/researcher department of Research Instituut Collectie Nederland/Netherlands Institute for Cultural Heritage Postbus 76709, 1070 KA Amsterdam/P.O. Box 76709, 1070 KA Amsterdam, The Netherlands T +31 (0)20 305 46 88/728 F +31 (0)20 305 47 00 E ineke.joosten-at-icn.nl
Het ICN is onderdeel van het Ministerie van OCW/ICN is part of the Ministry of Education, Culture and Science Zie voor meer informatie www.icn.nl/For more information see www. icn.nl Aan deze email kunnen geen rechten worden ontleend/No rights may be derived from this e-mail
==============================Original Headers============================== 11, 14 -- From ineke.joosten-at-icn.nl Mon Oct 17 10:31:58 2005 11, 14 -- Received: from mail.icn.nl (mail.icn.nl [213.201.149.20]) 11, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9HFVwdE005361 11, 14 -- for {Microscopy-at-Microscopy.Com} ; Mon, 17 Oct 2005 10:31:58 -0500 11, 14 -- Subject: Betr.: [Microscopy] viaWWW: JEOL Cross section polisher 11, 14 -- To: Microscopy-at-Microscopy.Com 11, 14 -- X-Mailer: Lotus Notes Release 5.0.11 July 24, 2002 11, 14 -- Message-ID: {OFA65E78D7.620151A3-ONC125709D.0054CFFB-C125709D.00555285-at-icn.nl} 11, 14 -- From: ineke.joosten-at-icn.nl 11, 14 -- Date: Mon, 17 Oct 2005 17:32:24 +0200 11, 14 -- X-MIMETrack: Serialize by Router on mailportal/icn(Release 6.0.3|September 26, 2003) at 11, 14 -- 10/17/2005 05:28:21 PM 11, 14 -- MIME-Version: 1.0 11, 14 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
this is an information for those members who are a part of a public / semi-public microanalysis service lab (electron microscope and/or x-ray spectrometer equipment). If there is the wish to put the service offer publicly, then you will find a new site to post your message without any fees:
The site has the aim to bring people with microanalytical problems together with them, who have the equipment and the possibility to solve the analytic task.
One of my colleagues is looking for an inexpensive fluorescent scope for screening cells.
If you have any personal experience with the Accu-Scope 3016 or another model (preferably with infinity corrected optics according to the brochure at http://www.microscopestore.com/info/3015.pdf ) and you are not a vendor of the microscope, we would greatly appreciate your feedback.
Thanks!
____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461 (718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/ **This electronic transmission contains information that is privileged.**
==============================Original Headers============================== 5, 23 -- From cammer-at-aecom.yu.edu Mon Oct 17 14:14:07 2005 5, 23 -- Received: from mailgw.aecom.yu.edu (mailgw.aecom.yu.edu [129.98.1.16]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9HJE7F2027287 5, 23 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2005 14:14:07 -0500 5, 23 -- Received: from mailvx.aecom.yu.edu (mailvx.aecom.yu.edu [129.98.1.17]) 5, 23 -- by mailgw.aecom.yu.edu (8.12.11/8.12.11) with SMTP id j9HJE2lB018797 5, 23 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2005 15:14:07 -0400 5, 23 -- Received: from post.aecom.yu.edu ([129.98.1.100]) 5, 23 -- by mailvx.aecom.yu.edu (SAVSMTP 3.1.1.32) with SMTP id M2005101715140721135 5, 23 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2005 15:14:07 -0400 5, 23 -- Received: from AIF3.aecom.yu.edu (aif3.aif.aecom.yu.edu [129.98.30.137]) 5, 23 -- by post.aecom.yu.edu (Postfix) with ESMTP id 243C72FC9; 5, 23 -- Mon, 17 Oct 2005 15:14:07 -0400 (EDT) 5, 23 -- Message-Id: {5.2.1.1.2.20051017151018.01c87c20-at-mailserver.aecom.yu.edu} 5, 23 -- X-Sender: cammer-at-mailserver.aecom.yu.edu 5, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 5, 23 -- Date: Mon, 17 Oct 2005 15:14:07 -0400 5, 23 -- To: microscopy-at-microscopy.com 5, 23 -- From: Michael Cammer {cammer-at-aecom.yu.edu} 5, 23 -- Subject: Accu-Scope 3016? 5, 23 -- Cc: wking-at-aecom.yu.edu 5, 23 -- Mime-Version: 1.0 5, 23 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I would be interested in what people use to neutralize their glutaraldehyde for hazardous waste removal. This would be 2.5% buffered glut. as used to fix human biopsy tissue.
Garry Burgess Charge Technologist Health Sciences Centre Winnipeg
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==============================Original Headers============================== 6, 20 -- From GBurgess-at-exchange.hsc.mb.ca Mon Oct 17 15:57:09 2005 6, 20 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca [142.233.100.122]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9HKv8Tf004839 6, 20 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2005 15:57:08 -0500 6, 20 -- Received: from hscxntmx0007.hsc.mb.ca (unverified [172.16.6.179]) by 6, 20 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS 4.0.346.0) with ESMTP id 6, 20 -- {B0015545172-at-hscxntmx0003.hsc.mb.ca} for {microscopy-at-microscopy.com} ;Mon, 6, 20 -- 17 Oct 2005 15:56:50 -0500 6, 20 -- Received: by hscxntmx0007.hsc.mb.ca with Internet Mail Service 6, 20 -- (5.5.2653.19)id {T7CKFWSJ} ; Mon, 17 Oct 2005 15:57:12 -0500 6, 20 -- Message-ID: 6, 20 -- {00A937989100304A83A058F6C45873FF32A2D4-at-hscxntmx0005.hsc.mb.ca} 6, 20 -- Date: Mon, 17 Oct 2005 15:55:44 -0500 6, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 6, 20 -- Subject: Neutralizing Glutaraldehyde 6, 20 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 6, 20 -- X-Mailer: Internet Mail Service (5.5.2653.19) 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="iso-8859-1" ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jeremiah.tyler-at-us.army.mil) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, October 17, 2005 at 16:16:22 ---------------------------------------------------------------------------
Question: I am pursuing a degree in criminal investigation and as an assignment I was to research the MSA website and submit my findings to my fellow students in a discussion board. I understand microscopy is a way of looking at items that are smaller than the eye can see and to get a grasp on the application of microscopy in regards to CSI is difficult for me since I am a visual person. What would be some ways an investigator could utilize the services of a criminal investigator?
I'm not sure of the exact question you are asking. But if you are asking how microscopy is used in the analysis of evidence, which is what I think you are trying to get at, here are a few examples. Our trace unit uses microscopy in most of it's analysis types. They use stereo microscopes extensively just to characterize the evidence. Phase contrast, polarized light and fluorescence microscopy is used for different types of evidence. An example of this is that fibers would be examined using a polarized light microscope to determine if it is natural or synthetic. They use a comparison microscope to compare the characteristics of a known and unknown fiber. The Scanning Electron Microscope/ Energy dispersive spectrometer (SEM/EDS) is used for both visual and elemental analysis of many evidence types, such as analysis of GSR from hands or to characterize the trace materials that prove ricochet when found embedded in the side of a bullet. Our Forensic Biology unit uses microscopes to exam for the presence of sperm in rape cases. We might use a video microscope unit to examine an area on a car for paint transfer of to help do examination and measurements in blood pattern analysis cases at a crime scene, it allows us to capture good documentation of the evidence in a nice portable unit (we do digital photos and film through many of our other scopes). Our drug chemistry unit uses microscopes to conduct crystal tests on some types of drugs, as one of several tests run. The unit I work in if Firearm and Toolmark analysis. We use stereo microscopes to examine bullets and cartridge cases for class characteristics. We use that same scope to characterize a defect in a garment as a bullet hole and to look for gun powder particle around the bullet hole as part of a range determination. After test firing a firearm we use a comparison microscope to compare the known bullet or cartridge case to the unknown (bullet/cartridge case form the scene) for both class and individual characteristics. We might be the one examining the bullet for proof of ricochet in the SEM. These are just a few examples of the many, many uses microscopes are put to in a modern Forensic Science lab. I hope this gives you an idea of what we do with microscopes related to physical evidence, if you have other questions contact me off the list.
Jim
James L. Roberts Firearm & Toolmark Examiner Ventura Co. Sheriff's Lab 800 S. Victoria Ave. Ventura, CA. 93009
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jeremiah.tyler-at-us.army.mil) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
on Monday, October 17, 2005 at 16:16:22 ---------------------------------------------------------------------------
Question: I am pursuing a degree in criminal investigation and as an assignment I was to research the MSA website and submit my findings to my fellow students in a discussion board. I understand microscopy is a way of looking at items that are smaller than the eye can see and to get a grasp on the application of microscopy in regards to CSI is difficult for me since I am a visual person. What would be some ways an investigator could utilize the services of a criminal investigator?
Here is a software I designed for Northwestern University. It is ASP-based, like to forum I am presenting the other day here (maybe I'd better not mention this: ).
Have a look: http://www.nuance.northwestern.edu/fom
Shuyou _____________________________ Shu-You Li, Ph.D. Electron Microscopist, EPIC NUANCE Northwestern University 2220 Campus Drive, 1161 Cook Hall (#2036 for mail) Evanston, IL 60208-3108, USA Ph: (847) 491-6723(O), (847) 491-7806(L), Fax: (847) 467-6573 Email: syli-at-northwestern.edu; shuyouli-at-gmail.com http://www.nuance.northwestern.edu/EPIChttp://www.shuyouli.com
==============================Original Headers============================== 6, 24 -- From syli-at-northwestern.edu Mon Oct 17 20:48:45 2005 6, 24 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57]) 6, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9I1mj7u002093 6, 24 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2005 20:48:45 -0500 6, 24 -- Received: from merle.it.northwestern.edu (localhost [127.0.0.1]) 6, 24 -- by merle.it.northwestern.edu (Postfix) with ESMTP id 739349E818 6, 24 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2005 20:48:44 -0500 (CDT) 6, 24 -- Content-Type: text/plain 6, 24 -- Content-Disposition: inline 6, 24 -- Content-Transfer-Encoding: binary 6, 24 -- X-Originating-Ip: 165.124.163.67 6, 24 -- Priority: 3 (Normal) 6, 24 -- X-Webmail-User: sli974-at-localhost 6, 24 -- To: microscopy-at-microscopy.com 6, 24 -- X-Priority: 3 (Normal) 6, 24 -- MIME-Version: 1.0 6, 24 -- X-Http_host: merle.it.northwestern.edu 6, 24 -- From: syli-at-northwestern.edu 6, 24 -- Subject: re: [Microscopy] viaWWW: online booking software 6, 24 -- Date: Tue, 18 Oct 2005 1:48:44 +0000 6, 24 -- Reply-To: syli-at-northwestern.edu 6, 24 -- X-Mailer: EMUmail 5.2.7 (UA Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 6, 24 -- 5.1; SV1; MyIE2; .NET CLR 1.1.4322)) 6, 24 -- Message-Id: {20051018014844.739349E818-at-merle.it.northwestern.edu} ==============================End of - Headers==============================
You ask for deactivation of used } glutaraldehyde { solution.....
there are methods which work at least in a "microcosmic-microchemical" environment (say cells, or fixation solutions used in tissue preparation for microscopy....): they all use either } glycine { solution, also } Na-borohydride { solutions can be used, and another alternative would be } ammonium-chloride { (NH4Cl) (hydrous or in the respective buffer) which is/has been used in immunohistochemistry (for a long while) for blocking free aldehyde groups (resting from prior fixation process within the tissue blocks).
For such a purpose, ROTH J et al in the 1980ies or 1990ies stated 50 mM hydrous solution of Ammoniumchloride (30 min) would be sufficient to block all free aldehyde groups in tissue blocks (the deactivation process takes - merely seen chemically - just a few seconds, but to be on the safe side for the deactivation of free aldehyde groups not bound to tissular elements in tissue blocks they recommended incubation of tissues for at least 30 mins at room temperature).
Concerning the concentration of such deactivating solutions for higher concentrated fixative solutions (say 2.5-4% GA) : perhaps / for sure excess in concentration used routinely for "microchemical" reaction are appropriate } usually { to be on the safe side.....
Since I have read some week ago that some commercially available deactivators of formaldehyde solutions (available in the USA, perhaps Canada too) like {Formalex} etc. are not suited for a deactivation of Glutaraldehyde, I use another deactivation method I was told many years ago,
namley highly alkalizing the (diluted) hydrous form- as well as glutar-aldehyde solution(s) by adding enough 10 N NaoH solution (which is also produced as a "waste" solution....after staining ultrathin sections you would have to } dispose of { sodiumhydroxide pellets.....I collect them into a bottle with water.....get nearly 5-10 N solution...and use it for such deactivation processes...) .....Unfortunately, I do not remember the source of that information as well as the efficiacy of such a deactivation.....
Also there have been reported other chemical reagents used for blocking aldehyde groups, namely sodium bisulfite as well as hydroxylamine (reagents I don't have experience with).
But now I tried GOOGLE search for "aldehyde deactivation" and came over some websites offering commercially available deactivation solutions, like: PREMIDEX (C) ADS2: deactivates GA-solutions up to 4% concentration: comp. http://www.metrex.com/products/unitedStates/premidexADS2/index.cfm or: found at: http://www.discount-office-supplies-resources.org/catalog1/Banta-Tissue- Drape.html
Shilog Product Catalog Sorted by Category (http://www.discount-office-supplies-resources.org/main.php?tracker=www. shilog.com/Web_Site_Store/catagory.html ) Shilog Medical Supply. P.O. Box 1887. 3551 Mt. Moriah Road. McAlester. Ok, 74502. Toll Free: 1-800-574-4868. McAlester: 1-918-423-4447. Fax: 918-423-5569. E-Mail Us. Aldehyde Deactivation System (perhaps this also points to PREMIDEX ADS2).
Also you can use the search phrase "aldehyde blocking" or "Formalin deactivation"...you will find again some references....
Regards
Wolfgang Muss Personal Communication Data: OR Dr. Wolfgang Muss Member of MSA, etc, FRMS Head of EM-Lab Institute of Pathology, SALK (Salzburger Landeskliniken gemeinnuetzige GesmbH) Muellner Hauptstrasse 48 A-5020 SALZBURG AUSTRIA/EUROPE
and/or/alternatively
Paracelsus Medical Private University (PMU) Institute of Pathology Electron Microscopy Lab Muellner Hauptstrasse 48 A-5020 SALZBURG, Austria/Europe Phone work: +43+662+4482+4720 Mobile phone work:+43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss") E-Mail work: W.Muss-at-SALK.at
Mobile-phone private: ++43+676+5 369-456 E-Mail private: wij.Muss-at-aon.at ------------------------------------------------------------------------ -------------------------------------- Ankuendigung namens der (Information on behalf of) Society for Cutaneous Ultrastructure Research (SCUR) PLEASE VISIT THE UPDATED WEBSITE of SCUR at } http://www.scur.org { ------------------------------------------------------------------------- Forthcoming Meetings: 33rd Annual Meeting of the SCUR, 8th-10th June, 2006, WARSZAW, Poland Preliminary informations: send an E-Mail kwoznia-at-amwaw.edu.pl
34th Annual Meeting of the SCUR, 11th-12th May, 2007, PRAGUE Czech Republic
35th Annual Meeting of the SCUR, 11th -12th May, 2008, NARA or KYOTO, Japan Joint Meeting with the JSUCB, the Japanese Society for Ultrastructural Cutaneous Biology
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I would be interested in what people use to neutralize their glutaraldehyde for hazardous waste removal. This would be 2.5% buffered glut. as used to fix human biopsy tissue.
Garry Burgess Charge Technologist Health Sciences Centre Winnipeg
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==============================Original Headers============================== 6, 20 -- From GBurgess-at-exchange.hsc.mb.ca Mon Oct 17 15:57:09 2005 6, 20 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca [142.233.100.122]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9HKv8Tf004839 6, 20 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2005 15:57:08 -0500 6, 20 -- Received: from hscxntmx0007.hsc.mb.ca (unverified [172.16.6.179]) by 6, 20 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS 4.0.346.0) with ESMTP id 6, 20 -- {B0015545172-at-hscxntmx0003.hsc.mb.ca} for {microscopy-at-microscopy.com} ;Mon, 6, 20 -- 17 Oct 2005 15:56:50 -0500 6, 20 -- Received: by hscxntmx0007.hsc.mb.ca with Internet Mail Service 6, 20 -- (5.5.2653.19)id {T7CKFWSJ} ; Mon, 17 Oct 2005 15:57:12 -0500 6, 20 -- Message-ID: 6, 20 -- {00A937989100304A83A058F6C45873FF32A2D4-at-hscxntmx0005.hsc.mb.ca} 6, 20 -- Date: Mon, 17 Oct 2005 15:55:44 -0500 6, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 6, 20 -- Subject: Neutralizing Glutaraldehyde 6, 20 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 6, 20 -- X-Mailer: Internet Mail Service (5.5.2653.19) 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="iso-8859-1" ==============================End of - Headers==============================
==============================Original Headers============================== 35, 28 -- From W.Muss-at-salk.at Tue Oct 18 04:11:28 2005 35, 28 -- Received: from Hermes.salk.at (hermes.lks.at [193.170.167.9]) 35, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9I9BQHs016074 35, 28 -- for {Microscopy-at-Microscopy.Com} ; Tue, 18 Oct 2005 04:11:27 -0500 35, 28 -- Received: from localhost (localhost [127.0.0.1]) 35, 28 -- by hermes.lks.at (Postfix) with ESMTP id 87DAE5A9022; 35, 28 -- Tue, 18 Oct 2005 11:08:54 +0200 (CEST) 35, 28 -- Received: from hermes.lks.at ([127.0.0.1]) 35, 28 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024) 35, 28 -- with ESMTP id 67156-10; Tue, 18 Oct 2005 11:08:54 +0200 (CEST) 35, 28 -- Received: from c1pa003 (unknown [192.168.42.3]) 35, 28 -- by hermes.lks.at (Postfix) with SMTP id 2C4E45A9020; 35, 28 -- Tue, 18 Oct 2005 11:08:54 +0200 (CEST) 35, 28 -- Received: by localhost with Microsoft MAPI; Tue, 18 Oct 2005 11:08:53 +0200 35, 28 -- Message-ID: {01C5D3D4.5386D540.W.Muss-at-salk.at} 35, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at} 35, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at} 35, 28 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-Microscopy.Com} 35, 28 -- Cc: "'GBurgess-at-exchange.hsc.mb.ca'" {GBurgess-at-exchange.hsc.mb.ca} 35, 28 -- Subject: [Microscopy] Re: Neutralizing Glutaraldehyde 35, 28 -- Date: Tue, 18 Oct 2005 11:08:52 +0200 35, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at} 35, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor 35, 28 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211 35, 28 -- MIME-Version: 1.0 35, 28 -- Content-Type: text/plain; charset="us-ascii" 35, 28 -- Content-Transfer-Encoding: 7bit 35, 28 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at ==============================End of - Headers==============================
When going beyond the apparently visual aspects of crime scenes, the trace evidence is where the microscopist would reside. For example, and these are just some items that are employed in crime scene processing, this list is not meant to be all inclusive.
1. Metals identification, e.g. plating from a bumper in a hit and run auto accident - metal fragments from knives 2. Identification of paints 3. Gunshot residue using SEM-EDX 4. Hair and fiber identification 5. Tool mark identification [pipe bombs for example and product tampering]
Along with the above there are other disciplines such as entomology that can analyze the type and progression of insects during the decomposition of the body thus pinning time of death and in some cases place of death. I should not leave out the most important which is DNA identification.
There is plenty of literature out there to help you.
A detective friend of mine told me that one can never enter and leave a crime scene without leaving something behind or exchanging something. Just think, you shed several million skin particles a day. One is bound to leave a few at the scene among other things.
Good luck in your studies.
Peter Tomic Ciclon Semiconductor Device Corp. Bethlehem, PA
-----Original Message----- X-from: jeremiah.tyler-at-us.army.mil [mailto:jeremiah.tyler-at-us.army.mil] Sent: Monday, October 17, 2005 6:52 PM To: ptomic-at-ciclonsemi.com
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jeremiah.tyler-at-us.army.mil) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, October 17, 2005 at 16:16:22 ---------------------------------------------------------------------------
Question: I am pursuing a degree in criminal investigation and as an assignment I was to research the MSA website and submit my findings to my fellow students in a discussion board. I understand microscopy is a way of looking at items that are smaller than the eye can see and to get a grasp on the application of microscopy in regards to CSI is difficult for me since I am a visual person. What would be some ways an investigator could utilize the services of a criminal investigator?
I was a forensic scientist for about nine years (back in the seventies) and was the supervisor of the trace evidence section of the lab. I used a variety of techniques including optical and scanning electron microscopy, x-ray diffraction, gas chromatography/mass spec, and x-ray fluorescence in addition to a number of physical test procedures. The key to working with a trace evidence lab is maintaining flexibility. Present your evidence and allow the analyst the opportunity to review it and suggest the appropriate testing protocols. While there are some standard approaches to the analysis of common evidence types such as paint, it pays to keep an open mind with regards to what exactly constitutes evidence and how that evidence might best be analyzed. The reason for this is that evidence plays two roles in the investigation/prosecution of a case. The first use of evidence is during the investigation phase of a case where the evidence often plays a part in the identification of a suspect. Here the analyst can often, though not always, be of significant help to the investigator by revealing information gleaned from the evidence such as hair color, or the color of a hit and run vehicle (maybe even the make and model in some situations), shoe size and brand (and by extension an idea of the height and weight of the suspect). The reason for keeping an open mind is that some types of tests performed and the information which results may be useful in identifying a suspect, but not in the second or prosecution phase of a case. Here is where cases can be made or lost depending on the care taken by the team of crime scene investigators and forensic scientists. There are strict rules of evidence which are designed to protect the innocent from either careless or willful tampering or contamination of the evidence. When one is concerned with particles of matter which are invisible until examined microscopically, there is a great risk of loss, contamination, co-mingling, or misinterpretation of that evidence. Also, the tests which are performed for the purpose of generating evidence to which testimony will be given in court must meet certain standards regarding admissability. Of course, the credentials of the analyst are paramount, but the tests must be generally accepted in the scientific community. Here, the use of the microscope is invaluable in that microscopical techniques for hair and fiber, paint, soil, gunshot residue, glass, tool marks, and firearm identification are well established and generally accepted in court when performed by competent and ethical analysts.
If you need more specific information on the nature of the evidence examined or the types of tests which can be performed you can contact me directlly at william.h.roberts-at-usa.dupont.com
Regards, Bill
jeremiah.tyler-at-us.army.mil wrote on 10/17/2005 06:48:19 PM:
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} } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (jeremiah.tyler-at-us.army.mil) from } http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html } on Monday, October 17, 2005 at 16:16:22 } --------------------------------------------------------------------------- } } Email: jeremiah.tyler-at-us.army.mil } Name: Jeremiah Tyler } } Organization: Central Texas College } } Education: Undergraduate College } } Location: Panama City Beach, FL } } Question: I am pursuing a degree in criminal investigation and as an } assignment I was to research the MSA website and submit my findings } to my fellow students in a discussion board. I understand microscopy } is a way of looking at items that are smaller than the eye can see } and to get a grasp on the application of microscopy in regards to CSI } is difficult for me since I am a visual person. What would be some } ways an investigator could utilize the services of a criminal } investigator? } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 7, 13 -- From zaluzec-at-microscopy.com Mon Oct 17 17:47:33 2005 } 7, 13 -- Received: from [10.71.6.97] (msdvpn25.msd.anl.gov [130.202.238.89]) } 7, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9HMlWBp015020 } 7, 13 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2005 17:47:32 -0500 } 7, 13 -- Mime-Version: 1.0 } 7, 13 -- X-Sender: (Unverified) } 7, 13 -- Message-Id: {p06020405bf79da5279bb-at-[10.71.6.97]} } 7, 13 -- Date: Mon, 17 Oct 2005 17:47:31 -0500 } 7, 13 -- To: microscopy-at-microscopy.com } 7, 13 -- From: jeremiah.tyler-at-us.army.mil (by way of Ask-A-Microscopist) } 7, 13 -- Subject: [Filtered] AskAMicroscopist: pursuing a degree in criminal } 7, 13 -- investigation } 7, 13 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
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==============================Original Headers============================== 15, 24 -- From William.H.Roberts-at-USA.dupont.com Tue Oct 18 09:52:43 2005 15, 24 -- Received: from mms21bas.mms.us.syntegra.com (relay21.mms.us.syntegra.com [192.12.251.10]) 15, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9IEqhtd004945 15, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Oct 2005 09:52:43 -0500 15, 24 -- Received: from mms24es.mms.us.syntegra.com (mms24es.mms.us.syntegra.com [150.143.232.70]) by mms21bas.mms.us.syntegra.com with ESMTP for Microscopy-at-microscopy.com; Tue, 18 Oct 2005 14:52:42 Z 15, 24 -- Received: from mms21bas.mms.us.syntegra.com (mms21bas.mms.us.syntegra.com [192.12.251.10]) by mms24es.mms.us.syntegra.com with ESMTP for Microscopy-at-microscopy.com; Tue, 18 Oct 2005 14:52:38 Z 15, 24 -- Received: from demhub21.lvs.dupont.com (demhub21.lvs.dupont.com [52.128.29.204]) by mms21bas.mms.us.syntegra.com with ESMTP for Microscopy-at-microscopy.com; Tue, 18 Oct 2005 14:52:38 Z 15, 24 -- Received: from unknown (HELO demhub1.lvs.dupont.com) (52.99.10.45) 15, 24 -- by demhub21.lvs.dupont.com with ESMTP; 18 Oct 2005 10:52:38 -0400 15, 24 -- X-DUPONT-INTERNET: Recieved via DuPont Internet Mail Relay 15, 24 -- Received: from cdcln05.lvs.dupont.com (52.99.26.10) 15, 24 -- by demhub1.lvs.dupont.com with ESMTP; 18 Oct 2005 10:52:38 -0400 15, 24 -- From: William H Roberts {William.H.Roberts-at-USA.dupont.com} 15, 24 -- In-Reply-To: {200510172248.j9HMmJHY016209-at-ns.microscopy.com} 15, 24 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: pursuing a degree in 15, 24 -- criminal 15, 24 -- To: Microscopy-at-microscopy.com 15, 24 -- X-Mailer: Lotus Notes Release 6.0.3 September 26, 2003 15, 24 -- Message-Id: {OFE76164E9.70BE786A-ON8525709E.004CA89D-8525709E.0051BA11-at-CDCLN05.LVS.DUPONT.COM} 15, 24 -- Date: Tue, 18 Oct 2005 10:52:35 -0400 15, 24 -- X-MIMETrack: Serialize by Router on CDCLNMH1/DuPont_MHUB(Release 6.0.5|March 15, 24 -- 27, 2005) at 10/18/2005 10:52:37 AM 15, 24 -- MIME-Version: 1.0 15, 24 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
We have just started doing time lapse imaging of cells in culture. As expected, the amount of data being produced is large. I expect to produce about 200 GB every couple weeks (if I am careful). The files are usually too large to be transfered to DVD (last night's acquisitions totaled more than 30GB).
I am considering getting an external drive case (USB2 and/or 1394 (firewire)) and every couple of weeks swapping a new drive into this case. The full drives would then go on a shelf for possible later data retrieval. Right now 200 GB internal drives can be had for about $70, so filling a couple dozen of these drives in a year would come to about $2000.
What do the rest of you do to store this sort of data?
---
Michael J. Herron, U of MN, Dept. of Entomology herro001-at-umn.edu 612-624-3688 (office) 612-625-5299 (FAX)
==============================Original Headers============================== 8, 15 -- From herro001-at-umn.edu Tue Oct 18 10:29:47 2005 8, 15 -- Received: from mtaout-c.tc.umn.edu (mtaout-c.tc.umn.edu [160.94.128.21]) 8, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9IFTk84014109 8, 15 -- for {Microscopy-at-sparc5.microscopy.com} ; Tue, 18 Oct 2005 10:29:47 -0500 8, 15 -- Received: from [134.84.48.158] (x84-48-158.ej2393.umn.edu [134.84.48.158]) by mtaout-c.tc.umn.edu with ESMTP; Tue, 18 Oct 2005 10:29:42 -0500 (CDT) 8, 15 -- X-Umn-Remote-Mta: [N] x84-48-158.ej2393.umn.edu [134.84.48.158] #+LO+TS+AU+HN 8, 15 -- Mime-Version: 1.0 (Apple Message framework v734) 8, 15 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 8, 15 -- Message-Id: {EE772AEB-27DE-4EF7-BCA1-02C8119340E8-at-umn.edu} 8, 15 -- Content-Transfer-Encoding: 7bit 8, 15 -- From: "Michael J. Herron" {herro001-at-umn.edu} 8, 15 -- Subject: Image storage? 8, 15 -- Date: Tue, 18 Oct 2005 10:29:40 -0500 8, 15 -- To: Microscopy {Microscopy-at-ns.microscopy.com} 8, 15 -- X-Mailer: Apple Mail (2.734) ==============================End of - Headers==============================
I would not put too much faith in anything that spins. Drives fail. If the data matters, I would think about something else. Tape is a much better option. It is more stable and each tape is cheeper. You may not pay for the increased cost of the tape drive with the savings of tape vs. HD, but the data will be more secure. I have set up an off site tape backup of my labs data (while I am at it I also backup some of the departmental data). Each night the new data is sucked off of the 1.5TB drive array that sits under my desk and is put on tape. This involves very little work for me, and great data safety. Even if the lab/building/university becomes a smoking whole in the ground, the data is off site and safe. You can always set up better systems with more money, mine is not the best, but it did not cost much and I sleep better at night. David
On Oct 18, 2005, at 8:32 AM, herro001-at-umn.edu wrote:
} } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } We have just started doing time lapse imaging of cells in culture. } As expected, the amount of data being produced is large. I expect to } produce about 200 GB every couple weeks (if I am careful). The files } are usually too large to be transfered to DVD (last night's } acquisitions totaled more than 30GB). } } I am considering getting an external drive case (USB2 and/or 1394 } (firewire)) and every couple of weeks swapping a new drive into this } case. The full drives would then go on a shelf for possible later } data retrieval. Right now 200 GB internal drives can be had for about } $70, so filling a couple dozen of these drives in a year would come } to about $2000. } } What do the rest of you do to store this sort of data? } } } --- } } Michael J. Herron, U of MN, Dept. of Entomology } herro001-at-umn.edu } 612-624-3688 (office) 612-625-5299 (FAX) } } } } ==============================Original } Headers============================== } 8, 15 -- From herro001-at-umn.edu Tue Oct 18 10:29:47 2005 } 8, 15 -- Received: from mtaout-c.tc.umn.edu (mtaout-c.tc.umn.edu } [160.94.128.21]) } 8, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j9IFTk84014109 } 8, 15 -- for {Microscopy-at-sparc5.microscopy.com} ; Tue, 18 Oct 2005 } 10:29:47 -0500 } 8, 15 -- Received: from [134.84.48.158] (x84-48-158.ej2393.umn.edu } [134.84.48.158]) by mtaout-c.tc.umn.edu with ESMTP; Tue, 18 Oct 2005 } 10:29:42 -0500 (CDT) } 8, 15 -- X-Umn-Remote-Mta: [N] x84-48-158.ej2393.umn.edu } [134.84.48.158] #+LO+TS+AU+HN } 8, 15 -- Mime-Version: 1.0 (Apple Message framework v734) } 8, 15 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; } format=flowed } 8, 15 -- Message-Id: {EE772AEB-27DE-4EF7-BCA1-02C8119340E8-at-umn.edu} } 8, 15 -- Content-Transfer-Encoding: 7bit } 8, 15 -- From: "Michael J. Herron" {herro001-at-umn.edu} } 8, 15 -- Subject: Image storage? } 8, 15 -- Date: Tue, 18 Oct 2005 10:29:40 -0500 } 8, 15 -- To: Microscopy {Microscopy-at-ns.microscopy.com} } 8, 15 -- X-Mailer: Apple Mail (2.734) } ==============================End of - } Headers==============================
==============================Original Headers============================== 5, 22 -- From Elliott-at-arizona.edu Tue Oct 18 10:59:46 2005 5, 22 -- Received: from smtpgate.email.arizona.edu (deagol.email.Arizona.EDU [128.196.133.142]) 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9IFxj82022997 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 18 Oct 2005 10:59:46 -0500 5, 22 -- Received: from localhost (faramir.email.arizona.edu [10.0.0.218]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 6FA46B75B29 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 18 Oct 2005 08:59:45 -0700 (MST) 5, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 0AD7AB72CE0 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 18 Oct 2005 08:59:44 -0700 (MST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v623) 5, 22 -- In-Reply-To: {200510181532.j9IFWMo4019956-at-ns.microscopy.com} 5, 22 -- References: {200510181532.j9IFWMo4019956-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {864d4079b0a77d1a1601f50a373bed5c-at-arizona.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: David Elliott {Elliott-at-arizona.edu} 5, 22 -- Subject: Re: [Microscopy] Image storage? 5, 22 -- Date: Tue, 18 Oct 2005 08:59:43 -0700 5, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 22 -- X-Mailer: Apple Mail (2.623) 5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Dear List, A colleague asked me to post this job opening.
The Aerospace Corporation in El Segundo, CA has an opening for a Member of the Technical Staff in the Microelectronics Technology Department.
JOB DUTIES:
Conduct basic and applied research using high resolution transmission electron microscopy (HR TEM), electron energy loss spectroscopy (EELS), electron diffraction, energy filtered imaging, electron tomography and other advanced TEM techniques. The successful candidate will be expected to develop innovative research programs involving the application of the state-of-the-art TEM techniques to materials research and microelectronic device analyses. Presentation of results in scientific meetings and publication in refereed journals is expected. The candidate will also be required to provide technical support to various US Air Force space programs. QUALIFICATIONS:
Ph.D. in physics, physical chemistry, materials science, electrical engineering, or related technical discipline required. Experience in characterizing materials and structures at the nanoscale is required. A strong background in solid state and device physics is essential. Ability to formulate and carry out independent research and targeted investigations is a crucial requirement. Expertise required in one or more of the following micro-analytical techniques: focused ion beam techniques, high-resolution transmission electron microscopy, electron energy-loss spectroscopy, or field-emission scanning electron microscopy. Ph.D. and/or Postdoctoral research experience shall include designing and conducting experimental studies, and performing data analyses. Additional background or expertise in mathematical analysis, device modeling and computer-based simulation is desirable. Candidates must have good oral communication and technical writing skills and the ability to work effectively as part of a team. US citizenship required.
Location: The Aerospace Corporation is located in El Segundo, CA, a beach community just outside of Los Angeles. Aerospace is 2 miles from Los Angeles International Airport. Employees at Aerospace experience the outstanding southern California climate, have access to year-round beach activities and ski resorts open in the local mountains during a substantial part of the year, as well as sporting and cultural activities in the greater Los Angeles area.
For further information, please contact Martin S. Leung or Steven C. Moss at
Martin S. Leung, Ph.D. The Aerospace Corporation P.O. Box 92957, MS/M2-244 Los Angeles, CA 90009-2957 Tel 310-336-7125 Email: martin.s.leung-at-aero.org
Steven C. Moss, Ph.D. The Aerospace Corporation P.O. Box 92957, MS/M2-244 Los Angeles, CA 90009-2957 Tel 310-336-9216 Email: steven.c.moss-at-aero.org
Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 14, 27 -- From tivol-at-caltech.edu Tue Oct 18 11:40:51 2005 14, 27 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 14, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9IGeoIm032107 14, 27 -- for {microscopy-at-msa.microscopy.com} ; Tue, 18 Oct 2005 11:40:50 -0500 14, 27 -- Received: from localhost (fire-dog [192.168.1.4]) 14, 27 -- by water-ox-postvirus (Postfix) with ESMTP 14, 27 -- id D01B4339ED; Tue, 18 Oct 2005 09:40:49 -0700 (PDT) 14, 27 -- Received: from earth-ox ([192.168.1.9]) 14, 27 -- by fire-dog (MailMonitor for SMTP v1.2.2 ) ; 14, 27 -- Tue, 18 Oct 2005 09:40:48 -0700 (PDT) 14, 27 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 14, 27 -- by earth-ox.its.caltech.edu (Postfix) with ESMTP 14, 27 -- id 38BFA109A40; Tue, 18 Oct 2005 09:40:48 -0700 (PDT) 14, 27 -- Mime-Version: 1.0 (Apple Message framework v623) 14, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 27 -- Message-Id: {bb6c128115821bd0406151d2b9380433-at-caltech.edu} 14, 27 -- Cc: martin.s.leung-at-aero.org 14, 27 -- From: Bill Tivol {tivol-at-caltech.edu} 14, 27 -- Subject: Job Opportunity at The Aeropspace Corp 14, 27 -- Date: Tue, 18 Oct 2005 09:44:38 -0700 14, 27 -- To: microscopy-at-msa.microscopy.com 14, 27 -- X-Mailer: Apple Mail (2.623) 14, 27 -- X-Spam-Status: No, hits=-8.4 tagged_above=-10000 required=5 14, 27 -- tests=[ALL_TRUSTED=-3.8, CIT_FROM_ADDR=-0.7, CIT_NET_FROM=-3.9] 14, 27 -- X-Spam-Level: 14, 27 -- Content-Transfer-Encoding: 8bit 14, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9IGeoIm032107 ==============================End of - Headers==============================
USB and Firewire drive controllers are somewhat flakey. So are the drives. I had a Maxtor that died about 6 months after new. The current nod is to Western Digital.
The quality of hard drives varies every year from maker to maker. One year one maker is tops, the next, down at the bottom. So, choose wisely.
The other problem is that drives don't like being turned on and off. For highest reliability, they should be left on all the time until tracks or sectors start to fail. When this happens, in a RAID system, the failing drive is replaced and the good data is mirrored back over. A single drive is a single point of failure.
A better option is to use tape. Best (IMO) is Ultrium 2 or 3. Next option is DLT. Drives are pricey. Media costs about $85 each. So once you get past the cost of the drive, the media is about the same as the hard drive. If you are really paranoid like me, I write two backup tape sets and store one off-site. I use NovaNET backup software and it will back up all PCs on my LAN. There is about 900GB of data stored.
gary g.
At 08:31 AM 10/18/2005, you wrote:
} We have just started doing time lapse imaging of cells in culture. } As expected, the amount of data being produced is large. I expect to } produce about 200 GB every couple weeks (if I am careful). The files } are usually too large to be transfered to DVD (last night's } acquisitions totaled more than 30GB). } } I am considering getting an external drive case (USB2 and/or 1394 } (firewire)) and every couple of weeks swapping a new drive into this } case. The full drives would then go on a shelf for possible later } data retrieval. Right now 200 GB internal drives can be had for about } $70, so filling a couple dozen of these drives in a year would come } to about $2000. } } What do the rest of you do to store this sort of data? } } } --- } } Michael J. Herron, U of MN, Dept. of Entomology } herro001-at-umn.edu } 612-624-3688 (office) 612-625-5299 (FAX)
==============================Original Headers============================== 12, 25 -- From gary-at-gaugler.com Tue Oct 18 11:55:08 2005 12, 25 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 12, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9IGt7KX008453 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 18 Oct 2005 11:55:07 -0500 12, 25 -- Received: (qmail 23942 invoked from network); 18 Oct 2005 09:54:43 -0700 12, 25 -- Received: by simscan 1.1.0 ppid: 23915, pid: 23916, t: 1.6936s 12, 25 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1095 spam: 3.0.3 12, 25 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 12, 25 -- by qsmtp1 with SMTP; 18 Oct 2005 09:54:41 -0700 12, 25 -- Message-Id: {6.2.3.4.2.20051018094138.02916c70-at-mail.calweb.com} 12, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 12, 25 -- Date: Tue, 18 Oct 2005 09:53:05 -0700 12, 25 -- To: herro001-at-umn.edu 12, 25 -- From: Gary Gaugler {gary-at-gaugler.com} 12, 25 -- Subject: Re: [Microscopy] Image storage? 12, 25 -- Cc: MSA listserver {microscopy-at-microscopy.com} 12, 25 -- In-Reply-To: {200510181531.j9IFVNVF017390-at-ns.microscopy.com} 12, 25 -- References: {200510181531.j9IFVNVF017390-at-ns.microscopy.com} 12, 25 -- Mime-Version: 1.0 12, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 12, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on 12, 25 -- qsmtp1.mc.surewest.net 12, 25 -- X-Spam-Level: 12, 25 -- X-Spam-Status: No, score=-2.2 required=5.0 tests=AWL,BAYES_00 autolearn=ham 12, 25 -- version=3.0.3 ==============================End of - Headers==============================
Hitschfel Instruments, Incorporated is a consultative sales company, providing microscope based imaging solutions to the biomedical research, bio-industrial, clinical and educational communities throughout the states of Missouri, Nebraska, Kansas, Oklahoma and Arkansas/Memphis plus central & southern Illinois.
We are seeking two exceptional and highly motivated individuals to work with us toward our mutual goals of success through customer satisfaction. The successful candidates for these Technical Sales positions will represent the Olympus microscope product line, as well as a variety of digital imaging hardware/software systems and other ancillary products within either our Kansas territory or our Arkansas/Memphis territory. These positions would carry the responsibilities of all aspects of the sales process to include initial contacts, consultative development of sales opportunities, product demonstrations, equipment delivery/assembly/in-service and after sales follow-up. You will also work in conjunction with our Imaging Technology Specialist to assist in the process of selling the most complex Image analysis and automated microscopy solutions.
The personal characteristics and qualifications that we are looking for are:
. A BS degree in the Life Sciences as a minimum . Biomedical research lab experience highly preferred . Microscopy and digital imaging skills highly preferred . Excellent communication and interpersonal skills . A high degree of self motivation and work ethic . Computer skills, including software/hardware installation
Previous sales experience would also be preferred, but if you are the right person for the job, we will teach you the selling skills that you will need to be successful.
This is an excellent commissioned sales career opportunity! We offer a competitive income potential, along with a liberal benefits package. If you are thinking about an alternative career in science that will put your knowledge of biomedical technology to use in the business world, please forward your resume with a cover letter to me at: dkinast-at-hitschfel.com.
David L. Kinast Sales Manager Hitschfel Instruments, Inc. 2333 South Hanley Rd. St. Louis, MO 63144 Phone:800/242-3501 Phone: 314/644-6660 Fax: 314/644-5877 dkinast-at-hitschfel.com http://www.hitschfel.com http://www.hitschfel.com/linecard.html http://www.olympusmicroscopes.com
==============================Original Headers============================== 13, 22 -- From dkinast-at-hitschfel.com Tue Oct 18 14:08:00 2005 13, 22 -- Received: from smtp102.biz.mail.mud.yahoo.com (smtp102.biz.mail.mud.yahoo.com [68.142.200.237]) 13, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9IJ7xEr019055 13, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Oct 2005 14:07:59 -0500 13, 22 -- Message-Id: {200510181907.j9IJ7xEr019055-at-ns.microscopy.com} 13, 22 -- Received: (qmail 74088 invoked from network); 18 Oct 2005 19:07:59 -0000 13, 22 -- Received: from unknown (HELO dkinastxps) (dkinast-at-hitschfel.com-at-67.66.147.195 with login) 13, 22 -- by smtp102.biz.mail.mud.yahoo.com with SMTP; 18 Oct 2005 19:07:59 -0000 13, 22 -- Reply-To: {dkinast-at-hitschfel.com} 13, 22 -- From: "David Kinast" {dkinast-at-hitschfel.com} 13, 22 -- To: {Microscopy-at-microscopy.com} 13, 22 -- Cc: {ghitschfel-at-hitschfel.com} 13, 22 -- Subject: LM Job Opportunities 13, 22 -- Date: Tue, 18 Oct 2005 14:07:14 -0500 13, 22 -- Organization: Hitschfel Instruments, Inc. 13, 22 -- MIME-Version: 1.0 13, 22 -- Content-Type: text/plain; 13, 22 -- charset="US-ASCII" 13, 22 -- Content-Transfer-Encoding: 7bit 13, 22 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353 13, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2527 13, 22 -- Thread-Index: AcXUFyaNBao3AGhRSjKm7rhOba5uzA== ==============================End of - Headers==============================
Also have a look at hot swappable hard drive cages (see below).
X-from my personal experience of tape backup, not only is it very slow and expensive, I have abandoned it due to reliability problems: the only two times I really needed it the archived tapes were 'corrupted. I have since given up with any form of file compression for the same reason.
In comparison we haven't had any hard drive fail on the 12 PC's we use for imaging and they are all guaranteed for 5 years, and we have up to four hard drives per PC - although upgrade the power supply [PSU] to 450w+ from the paltry ones normally supplied. Being 'poor', I often use ebay for very cheap processor upgrades to the max the motherboard can take and crucial.com for more memory (although be warned Windows 98SE & ME OS can only handle 512Mb max). I always avoid Fujitsu drives from experience, and I tend towards Western Digital from habit, rather than performance, and as I'm used to their website for utilities. External hard drives are also rather slow, even with firewire and USB2, and I tend not to trust them. I only use them for occasional transfer between PC's as they are much faster than the network. The only problem we have is our older PC's have internal hard drive BIOS limits e.g. 128Gb per hard drive, which can only be overcome by a tacky. I tend to have S.M.A.R.T. on, which should help identify failing drives.
Hot swappable drive cages
The option I would have a look at is internal hot-swappable SATA hard drive cages (or IDE depending on motherboards) as these are cheap and reliable per Gb. I never use SCSI anymore as the cost of these is prohibitive per Gb and they aren't even significantly faster anymore. You can be clever and use raid etc.. but we don't, we just backup to a second media (normally optical with data verification) as soon as the data is collected.
With hot swappable hard drive cages you simply pull out the hard drives and replace them rather like floppy disks. Being hot swappable you can naturally do this while the PC is running (they are designed for servers). But of course you can do it while the system is off. e.g. http://www.rackmount.com/Rackacc/HDC-300.htm
The OS remains on another internal drive.
That is a lot of data you are producing though, each of our OpenLabs or confocal time-lapse systems produce far smaller total file sizes per week, so DVD's and CD's are OK for us at the moment. User's here are responsible for their own backup, although I provide more than adequate storage space (300Gb+ per PC) and full back-up facilities for them - after all only they know which images are important.
Regards
Keith
==============================Original Headers============================== 13, 28 -- From keith.morris-at-ucl.ac.uk Wed Oct 19 03:58:36 2005 13, 28 -- Received: from vscani-a.ucl.ac.uk (vscani-a.ucl.ac.uk [144.82.108.29]) 13, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9J8wam8006226 13, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Oct 2005 03:58:36 -0500 13, 28 -- Received: from cell23.ioo.ucl.ac.uk ([144.82.134.123] helo=keithhigrade) 13, 28 -- by vscani-a.ucl.ac.uk with smtp (Exim 4.51) 13, 28 -- id 1ES9mH-0006y1-9A 13, 28 -- for Microscopy-at-microscopy.com; Wed, 19 Oct 2005 09:58:29 +0100 13, 28 -- Message-ID: {002901c5d48b$36365440$7b865290-at-keithhigrade} 13, 28 -- From: "Keith J Morris" {keith.morris-at-ucl.ac.uk} 13, 28 -- To: {Microscopy-at-microscopy.com} 13, 28 -- References: {200510181700.j9IH0QPY016299-at-ns.microscopy.com} 13, 28 -- Subject: Re: [Microscopy] Re: Image storage? 13, 28 -- Date: Wed, 19 Oct 2005 09:58:02 +0100 13, 28 -- MIME-Version: 1.0 13, 28 -- Content-Type: text/plain; 13, 28 -- format=flowed; 13, 28 -- charset="iso-8859-1"; 13, 28 -- reply-type=original 13, 28 -- Content-Transfer-Encoding: 7bit 13, 28 -- X-Priority: 3 13, 28 -- X-MSMail-Priority: Normal 13, 28 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2670 13, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2670 13, 28 -- X-UCL-MailScanner-Information: Please contact the UCL Helpdesk, helpdesk-at-ucl.ac.uk for more information 13, 28 -- X-UCL-MailScanner: Found to be clean 13, 28 -- X-UCL-MailScanner-SpamCheck: 13, 28 -- X-MailScanner-From: keith.morris-at-ucl.ac.uk ==============================End of - Headers==============================
Well, it's all about how paranoid you want to be...
Here, we back up our data daily with an incremental backup, then do a full backup on a regular basis, and then we backup the backup to a different site. Just in case the building burns down....
Regarding disk drives and tapes: Perhaps you have been lucky, but the mean time between failures for hard disks is not infinite. It is not a quuestion of if, but rather when a hard disk will fail. And invariably they fail at the most inopportune moment. I had my hard disk crash on my laptop during M&M this year, so I had to do heart surgery on my laptop on the show floor and limp along for a week until I could get back to our network and get it back in full working condition. We use a RAID system here to store data, and we lose a disk once in a while. Because we are using RAID, it is not a big deal. Take out the old disk, plug in a new one, and have the system rebuild the information on the new disk.
If you use a tape for backup, it is a good idea to run a couple of tests before you put it away to make sure the tape is OK, and store it in a place where the data doesn't deteriorate (climate controlled, no strong magnetic fields).
Hot swappable drives are good, but not fail-safe, unless you use 2 copies. We use them here, too, and have not had any problems, but again, it's a normal disk drive and will fail eventually. By using multiple drives and rotating them, you can probably extend the mean time between failure to a timeframe that is long enough for you.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: keith.morris-at-ucl.ac.uk [mailto:keith.morris-at-ucl.ac.uk] Sent: Wednesday, October 19, 2005 3:03 AM To: Mike Bode
Also have a look at hot swappable hard drive cages (see below).
X-from my personal experience of tape backup, not only is it very slow and expensive, I have abandoned it due to reliability problems: the only two times I really needed it the archived tapes were 'corrupted. I have since given up with any form of file compression for the same reason.
In comparison we haven't had any hard drive fail on the 12 PC's we use for imaging and they are all guaranteed for 5 years, and we have up to four hard drives per PC - although upgrade the power supply [PSU] to 450w+ from the paltry ones normally supplied. Being 'poor', I often use ebay for very cheap processor upgrades to the max the motherboard can take and crucial.com for more memory (although be warned Windows 98SE & ME OS can only handle 512Mb max). I always avoid Fujitsu drives from experience, and I tend towards Western Digital from habit, rather than performance, and as I'm used to their website for utilities. External hard drives are also rather slow, even with firewire and USB2, and I tend not to trust them. I only use them for occasional transfer between PC's as they are much faster than the network. The only problem we have is our older PC's have internal hard drive BIOS limits e.g. 128Gb per hard drive, which can only be overcome by a tacky. I tend to have S.M.A.R.T. on, which should help identify failing drives.
Hot swappable drive cages
The option I would have a look at is internal hot-swappable SATA hard drive cages (or IDE depending on motherboards) as these are cheap and reliable per Gb. I never use SCSI anymore as the cost of these is prohibitive per Gb and they aren't even significantly faster anymore. You can be clever and use raid etc.. but we don't, we just backup to a second media (normally optical with data verification) as soon as the data is collected.
With hot swappable hard drive cages you simply pull out the hard drives and replace them rather like floppy disks. Being hot swappable you can naturally do this while the PC is running (they are designed for servers). But of course you can do it while the system is off. e.g. http://www.rackmount.com/Rackacc/HDC-300.htm
The OS remains on another internal drive.
That is a lot of data you are producing though, each of our OpenLabs or confocal time-lapse systems produce far smaller total file sizes per week, so DVD's and CD's are OK for us at the moment. User's here are responsible for their own backup, although I provide more than adequate storage space (300Gb+ per PC) and full back-up facilities for them - after all only they know which images are important.
Regards
Keith
==============================Original Headers============================== 13, 28 -- From keith.morris-at-ucl.ac.uk Wed Oct 19 03:58:36 2005 13, 28 -- Received: from vscani-a.ucl.ac.uk (vscani-a.ucl.ac.uk [144.82.108.29]) 13, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9J8wam8006226 13, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Oct 2005 03:58:36 -0500 13, 28 -- Received: from cell23.ioo.ucl.ac.uk ([144.82.134.123] helo=keithhigrade) 13, 28 -- by vscani-a.ucl.ac.uk with smtp (Exim 4.51) 13, 28 -- id 1ES9mH-0006y1-9A 13, 28 -- for Microscopy-at-microscopy.com; Wed, 19 Oct 2005 09:58:29 +0100 13, 28 -- Message-ID: {002901c5d48b$36365440$7b865290-at-keithhigrade} 13, 28 -- From: "Keith J Morris" {keith.morris-at-ucl.ac.uk} 13, 28 -- To: {Microscopy-at-microscopy.com} 13, 28 -- References: {200510181700.j9IH0QPY016299-at-ns.microscopy.com} 13, 28 -- Subject: Re: [Microscopy] Re: Image storage? 13, 28 -- Date: Wed, 19 Oct 2005 09:58:02 +0100 13, 28 -- MIME-Version: 1.0 13, 28 -- Content-Type: text/plain; 13, 28 -- format=flowed; 13, 28 -- charset="iso-8859-1"; 13, 28 -- reply-type=original 13, 28 -- Content-Transfer-Encoding: 7bit 13, 28 -- X-Priority: 3 13, 28 -- X-MSMail-Priority: Normal 13, 28 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2670 13, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2670 13, 28 -- X-UCL-MailScanner-Information: Please contact the UCL Helpdesk, helpdesk-at-ucl.ac.uk for more information 13, 28 -- X-UCL-MailScanner: Found to be clean 13, 28 -- X-UCL-MailScanner-SpamCheck: 13, 28 -- X-MailScanner-From: keith.morris-at-ucl.ac.uk ==============================End of - Headers==============================
==============================Original Headers============================== 28, 24 -- From Mike.Bode-at-soft-imaging.net Wed Oct 19 10:11:26 2005 28, 24 -- Received: from mail.soft-imaging.de (mail.soft-imaging.de [62.180.61.131]) 28, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9JFBP0o020034 28, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Oct 2005 10:11:25 -0500 28, 24 -- Received: from muenster.soft-imaging.net (muenster.soft-imaging.de [192.168.118.231]) 28, 24 -- by mail.soft-imaging.de (8.11.6/8.11.6/SuSE Linux 0.5) with ESMTP id j9JFBOH26004; 28, 24 -- Wed, 19 Oct 2005 17:11:25 +0200 28, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 28, 24 -- Content-class: urn:content-classes:message 28, 24 -- MIME-Version: 1.0 28, 24 -- Content-Type: text/plain; 28, 24 -- charset="iso-8859-1" 28, 24 -- Subject: RE: [Microscopy] Image storage? 28, 24 -- Date: Wed, 19 Oct 2005 17:09:19 +0200 28, 24 -- Message-ID: {6D0150089E1EA046BD96C68C50608C23020982AB-at-ms-s-gws.soft-imaging.net} 28, 24 -- X-MS-Has-Attach: 28, 24 -- X-MS-TNEF-Correlator: 28, 24 -- Thread-Topic: [Microscopy] Image storage? 28, 24 -- Thread-Index: AcXUi//SeEG0e+RVScGJrPFRH7ejuAAMUtkw 28, 24 -- From: "Mike Bode" {Mike.Bode-at-soft-imaging.net} 28, 24 -- To: {Microscopy-at-microscopy.com} 28, 24 -- Cc: {keith.morris-at-ucl.ac.uk} 28, 24 -- Content-Transfer-Encoding: 8bit 28, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9JFBP0o020034 ==============================End of - Headers==============================
We are using a LaCie Biggest Disk array. It has four 250GB hard drives in a raid 5 configuration (Raid 0 and 1 are options) netting 750GB. It has a Firewire 800 and USB 2.0 interface so installation is trivial. The only problem we have seen is that the Firewire is quite slow on our old computer that also runs the Gatan camera and GIF. It is fast on USB 2.0 on another computer. Vendor support did not go to help debug the computer, since the drive seemed to be working. There is more on: http://www.lacie.com/products/ On this system, if one spindle fails, the data can be recovered since it is redundant on the other spindles. Tape is theoretically good too, but don't be overconfident that you can restore from a tape that has been sitting on a shelf for a few years.
John Mardinly Intel Corporation
The opinions of this author do not necessarily reflect the opinons of Intel Corporation.
-----Original Message----- X-from: herro001-at-umn.edu [mailto:herro001-at-umn.edu] Sent: Tuesday, October 18, 2005 8:30 AM To: Mardinly, John
We have just started doing time lapse imaging of cells in culture. As expected, the amount of data being produced is large. I expect to produce about 200 GB every couple weeks (if I am careful). The files are usually too large to be transfered to DVD (last night's acquisitions totaled more than 30GB).
I am considering getting an external drive case (USB2 and/or 1394 (firewire)) and every couple of weeks swapping a new drive into this case. The full drives would then go on a shelf for possible later data retrieval. Right now 200 GB internal drives can be had for about $70, so filling a couple dozen of these drives in a year would come to about $2000.
What do the rest of you do to store this sort of data?
---
Michael J. Herron, U of MN, Dept. of Entomology herro001-at-umn.edu 612-624-3688 (office) 612-625-5299 (FAX)
==============================Original Headers============================== 8, 15 -- From herro001-at-umn.edu Tue Oct 18 10:29:47 2005 8, 15 -- Received: from mtaout-c.tc.umn.edu (mtaout-c.tc.umn.edu [160.94.128.21]) 8, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9IFTk84014109 8, 15 -- for {Microscopy-at-sparc5.microscopy.com} ; Tue, 18 Oct 2005 10:29:47 -0500 8, 15 -- Received: from [134.84.48.158] (x84-48-158.ej2393.umn.edu [134.84.48.158]) by mtaout-c.tc.umn.edu with ESMTP; Tue, 18 Oct 2005 10:29:42 -0500 (CDT) 8, 15 -- X-Umn-Remote-Mta: [N] x84-48-158.ej2393.umn.edu [134.84.48.158] #+LO+TS+AU+HN 8, 15 -- Mime-Version: 1.0 (Apple Message framework v734) 8, 15 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 8, 15 -- Message-Id: {EE772AEB-27DE-4EF7-BCA1-02C8119340E8-at-umn.edu} 8, 15 -- Content-Transfer-Encoding: 7bit 8, 15 -- From: "Michael J. Herron" {herro001-at-umn.edu} 8, 15 -- Subject: Image storage? 8, 15 -- Date: Tue, 18 Oct 2005 10:29:40 -0500 8, 15 -- To: Microscopy {Microscopy-at-ns.microscopy.com} 8, 15 -- X-Mailer: Apple Mail (2.734) ==============================End of - Headers==============================
==============================Original Headers============================== 19, 33 -- From john.mardinly-at-intel.com Wed Oct 19 10:51:50 2005 19, 33 -- Received: from scsfmr002.sc.intel.com (fmr22.intel.com [143.183.121.14]) 19, 33 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9JFpoed029137 19, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Oct 2005 10:51:50 -0500 19, 33 -- Received: from scsfmr100.sc.intel.com (scsfmr100.sc.intel.com [10.3.253.9]) 19, 33 -- by scsfmr002.sc.intel.com (8.12.10/8.12.10/d: major-outer.mc,v 1.1 2004/09/17 17:50:56 root Exp $) with ESMTP id j9JFpnCv018147 19, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Oct 2005 15:51:49 GMT 19, 33 -- Received: from scsmsxvs041.sc.intel.com (scsmsxvs041.sc.intel.com [10.3.90.10]) 19, 33 -- by scsfmr100.sc.intel.com (8.12.10/8.12.10/d: major-inner.mc,v 1.2 2004/09/17 18:05:01 root Exp $) with SMTP id j9J91kW5022730 19, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Oct 2005 09:02:13 GMT 19, 33 -- Received: from scsmsx332.amr.corp.intel.com ([10.3.90.6]) 19, 33 -- by scsmsxvs041.sc.intel.com (SAVSMTP 3.1.7.47) with SMTP id M2005101908514925564 19, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Oct 2005 08:51:49 -0700 19, 33 -- Received: from scsmsx403.amr.corp.intel.com ([10.3.90.18]) by scsmsx332.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.211); 19, 33 -- Wed, 19 Oct 2005 08:51:49 -0700 19, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 19, 33 -- Content-class: urn:content-classes:message 19, 33 -- MIME-Version: 1.0 19, 33 -- Content-Type: text/plain; 19, 33 -- charset="us-ascii" 19, 33 -- Subject: FW: [Microscopy] Image storage? 19, 33 -- Date: Wed, 19 Oct 2005 08:51:48 -0700 19, 33 -- Message-ID: {9E1ED6A623D8CB44B4866ACF20ECDB2B07E93CFF-at-scsmsx403.amr.corp.intel.com} 19, 33 -- X-MS-Has-Attach: 19, 33 -- X-MS-TNEF-Correlator: 19, 33 -- Thread-Topic: [Microscopy] Image storage? 19, 33 -- Thread-Index: AcXT+MtSqWkY2Ci3Q1qDWIW+HgX2yAAeVJYwAAC+r/AAE8sWkA== 19, 33 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 19, 33 -- To: {Microscopy-at-MSA.Microscopy.Com} 19, 33 -- X-OriginalArrivalTime: 19 Oct 2005 15:51:49.0333 (UTC) FILETIME=[04286850:01C5D4C5] 19, 33 -- X-Scanned-By: MIMEDefang 2.52 on 10.3.253.9 19, 33 -- Content-Transfer-Encoding: 8bit 19, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9JFpoed029137 ==============================End of - Headers==============================
Hi Listers, does anyone have a copy of the Micrion focused ion beam microscope service manual, part number 800 000033, "Service Procedures 2000 Manual" which they would be willing to copy (or lend to me so I can copy and return)? I'm happy to pay any costs involved. I have tried via FEI but they are unable to help me, so I figure the listserver is my best hope.
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==============================Original Headers============================== 7, 28 -- From richard.beanland-at-bookham.com Thu Oct 20 10:58:52 2005 7, 28 -- Received: from mail82.messagelabs.com (mail82.messagelabs.com [195.245.231.67]) 7, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9KFwpD1032520 7, 28 -- for {microscopy-at-microscopy.com} ; Thu, 20 Oct 2005 10:58:52 -0500 7, 28 -- X-VirusChecked: Checked 7, 28 -- X-Env-Sender: richard.beanland-at-bookham.com 7, 28 -- X-Msg-Ref: server-2.tower-82.messagelabs.com!1129823930!38157572!1 7, 28 -- X-StarScan-Version: 5.4.15; banners=bookham.com,-,- 7, 28 -- X-Originating-IP: [213.249.209.179] 7, 28 -- Received: (qmail 10740 invoked from network); 20 Oct 2005 15:58:50 -0000 7, 28 -- Received: from unknown (HELO cas-smx-brdg-01.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 7, 28 -- by server-2.tower-82.messagelabs.com with SMTP; 20 Oct 2005 15:58:50 -0000 7, 28 -- Content-class: urn:content-classes:message 7, 28 -- MIME-Version: 1.0 7, 28 -- Content-Type: text/plain; 7, 28 -- charset="iso-8859-1" 7, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 7, 28 -- Subject: Micrion Service Manual needed 7, 28 -- Date: Thu, 20 Oct 2005 16:58:50 +0100 7, 28 -- Message-ID: {FF719652ABB4414D86916ACAAE5BBB24015FBD8A-at-cas-smx-01.caswell1.europe.bkhm.net} 7, 28 -- X-MS-Has-Attach: 7, 28 -- X-MS-TNEF-Correlator: 7, 28 -- Thread-Topic: Micrion Service Manual needed 7, 28 -- Thread-Index: AcXVjmO4HRaTbD01SO+B9o56NNs3tgAANWmw 7, 28 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 7, 28 -- To: {microscopy-at-microscopy.com} 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9KFwpD1032520 ==============================End of - Headers==============================
We have a JEOL Model JEM-100C TEM that is to be deinstalled next week. It is presently located in south Texas. If there is any interest in acquiring this instrument please contact me off line asap.
Bob Roberts EM Lab Services, Inc. 449 NW 62nd St. Topeka, Kansas 66617-1780 785.246.1232 voice 785.246.0168 fax www.emlabservices.com
==============================Original Headers============================== 5, 23 -- From emlabservices-at-cox.net Thu Oct 20 15:26:17 2005 5, 23 -- Received: from centrmmtao02.cox.net (centrmmtao02.cox.net [70.168.83.82]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9KKQHA0012416 5, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Oct 2005 15:26:17 -0500 5, 23 -- Received: from EMLabServices ([24.255.213.54]) by centrmmtao02.cox.net 5, 23 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with SMTP 5, 23 -- id {20051020202614.ZKVP21553.centrmmtao02.cox.net-at-EMLabServices} 5, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Oct 2005 16:26:14 -0400 5, 23 -- Message-ID: {002101c5d5b4$85d8e1a0$6500a8c0-at-EMLabServices} 5, 23 -- From: "EM Lab Services" {emlabservices-at-cox.net} 5, 23 -- To: {Microscopy-at-microscopy.com} 5, 23 -- Subject: TEM -- JEOL JEM-100C Free 5, 23 -- Date: Thu, 20 Oct 2005 13:26:16 -0700 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- format=flowed; 5, 23 -- charset="iso-8859-1"; 5, 23 -- reply-type=original 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- X-Priority: 3 5, 23 -- X-MSMail-Priority: Normal 5, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 5, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Stacey.Andringa-at-uc.edu) from http://www.microscopy.com/MLFormMail.html on Thursday, October 20, 2005 at 08:37:17 ---------------------------------------------------------------------------
Question: Does anyone have an old working digitizing tablet and mouse that needs a good home? Our old tablet reached "end of life" nine years ago, but just now died. We are on a tight budget and have been looking for a used one. Thanks in advance for any help.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mbisher-at-princeton.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Thursday, October 20, 2005 at 08:54:15 ---------------------------------------------------------------------------
Email: mbisher-at-princeton.edu Name: Margaret Bisher
Organization: Princeton University
Title-Subject: [Filtered] MListserver:
Question: Here we are looking for staining information for the polymer particles we would like to look at under TEM. The polymer particles are dispersed on the TEM grid coated with holy carbon film. The particles are composed of Poly (Ethylene glycol) PEG) + b-Poly(carprolactone)(PCL) (5k-7k). The average diameter of particles is about 200nm. The diameter of the core of the particles (PEG) is about 140nm, and the polymer brush(PCL)outside core is about 30nm long.
Yes external Hard drive cages do exist, but if you are going 'external' a few 300Gb+ Lacie or Maxtor/WesternDigital Firewire/USB2 drives may be easier and probably cheaper (and they are fairly 'hot swappable' as well by simply unplugging the USB2/Firewire lead).
Have a look at the £230 LaCie 500GB Big Disk Extreme FW400/800 USB2 7200rpm 16MB cache or the £500 LaCie 1000GB Bigger Disk Extreme 7200rpm Fast Firewire (seems to have been discountinued).
There is also the Lacie two Terabyte fully hot swappable F800 HD 'cartridge' system, if you must, but then you are going over £1,500 for the total convenience of hot-swappable drives (with the USB2 still plugged in). LaCie offers a spare drives and drawers (just like the internal cages) for use with the LaCie Biggest F800. You can immediately and easily hot-swap a failed drive etc. These Lacie products are faster than most if not all other USB/Firewire external units, and are highly regarded.
See it all at: http://www.lacie.com/products/range.htm?id=10033
The large number of hard drives enclosed in the Lacie non 'hot-swappable' HD units makes them trans-staggerable rather than portable though.
External Ultra-SCSI HD cages would also be ideal, fed via a SCSI out PCI card, but the price of SCSI drives is totally prohibitive per Gb.
With regard to internal PC heat, our PC's have extra fans in cabinets (although they aren't very effective), but being mostly fairly elderly the CPU's don't generate the heat of latest PC's. Upgrading the power supply (PSU) is a big improvement as these also have temperature control and superior extraction fans (and most case's heat-flow are designed to have heat exit this way). I regularly remove dust bunnies from inside the PC's as well and the PC's are kept on benches rather than the floor to reduce dust contamination (dust insulation causes temperature build-up). An air-jet plus Hoover works fine (but avoid Hoovering electronics directly to prevent static build up).
Regards
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
----- Original Message ----- X-from: "Michael Herron" {herro001-at-umn.edu} To: {keith.morris-at-ucl.ac.uk} Sent: Wednesday, October 19, 2005 11:24 PM
Electron Backscattering Diffraction in the Scanning Electron Microscope
Immediate vacancy for a post doc.
My post doc is leaving me to go to a great job with General Electric, so I have a year and a half of funding with no one to do the work. I am looking for a post doc, starting immediately (or as soon as is practical) and ending May 31, 2007.
This is a DOE-funded project entitled: Strain Measurement in Thin Films. The main focus of the work is developing and extending the technique of EBSD in the SEM. The work involves EBSD, energy-filtered EBSD, and writing new procedures for analyzing data. A new SEM that will be chosen to give optimum EBSD performance will be ordered before the end of the year to supplement the already very strong set of instruments available at Lehigh for this work. You can get some idea of what is wanted from looking at previous work from this group: Microscopy and Microanalysis 11 (2005) 341-353 Microscopy and Microanalysis 11 Supplement 2 (2005) 524-525
The ideal candidate will have prior experience of three kinds: experimental electron microscopy, dynamical diffraction theory and programming.
The next steps are a) to further the use of EBSD to measure strain and to apply it to electromigration, b) to develop better spatial resolution in EBSD through energy filtering and c) to write software to simulate EBSD patterns.
Candidates interested in this post please contact me. If you have sent me email enquiring about a job and have got a reply saying I have no vacancies (which was true at the time), please feel free to send your details again - I have deleted the information you sent previously.
Alwyn Eades
-- ........... Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
==============================Original Headers============================== 11, 23 -- From jae5-at-lehigh.edu Fri Oct 21 07:43:57 2005 11, 23 -- Received: from rain.CC.Lehigh.EDU (rain.CC.Lehigh.EDU [128.180.39.20]) 11, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9LChvxG029226 11, 23 -- for {microscopy-at-microscopy.com} ; Fri, 21 Oct 2005 07:43:57 -0500 11, 23 -- Received: from [128.180.55.141] (Dyn055141.mat.Lehigh.EDU [128.180.55.141]) 11, 23 -- (authenticated bits=0) 11, 23 -- by rain.CC.Lehigh.EDU (8.13.5/8.13.5) with ESMTP id j9LChvxB009866 11, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 11, 23 -- for {microscopy-at-microscopy.com} ; Fri, 21 Oct 2005 08:43:57 -0400 11, 23 -- Message-ID: {4358E28D.6030402-at-lehigh.edu} 11, 23 -- Date: Fri, 21 Oct 2005 08:43:57 -0400 11, 23 -- From: Alwyn Eades {jae5-at-lehigh.edu} 11, 23 -- Organization: Lehigh University 11, 23 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 11, 23 -- X-Accept-Language: en-us, en 11, 23 -- MIME-Version: 1.0 11, 23 -- To: microscopy-at-microscopy.com 11, 23 -- Subject: Vacancy for a post doc 11, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 23 -- Content-Transfer-Encoding: 7bit 11, 23 -- X-Greylist: Sender succeeded SMTP AUTH authentication, not delayed by milter-greylist-2.0 (rain.CC.Lehigh.EDU [128.180.39.20]); Fri, 21 Oct 2005 08:43:57 -0400 (EDT) 11, 23 -- X-Virus-Scanned: ClamAV version 0.87, clamav-milter version 0.87 on rain.CC.Lehigh.EDU 11, 23 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (familia_zepeda-at-yahoo.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, October 20, 2005 at 22:15:54 ---------------------------------------------------------------------------
Question: I am doing a research project on William Nichol who invented the polorizing microscope, but i cant seem to find any biographical information on him is there anyway you can help me?
Try the correct name of the guy, which in fact is } NICOL {, Search for "Nicol William" or, perhaps better, Nicol prism....you will get some results..... eg. on WIKIPEDIA http://en.wikipedia.org/wiki/Nicol_prism
==} } "......It was invented in 1828 by William Nicol (1768-1851) of Edinburgh...." etc..
Best regards
Wolfgang Muss
Personal Communication Data: OR Dr. Wolfgang Muss Member of EM-Lab Institute of Pathology, SALK (Salzburger Landeskliniken gemeinnuetzige GesmbH) Muellner Hauptstrasse 48 A-5020 SALZBURG AUSTRIA/EUROPE
and/or/alternatively
Paracelsus Medical Private University (PMU) Institute of Pathology Electron Microscopy Lab Muellner Hauptstrasse 48 A-5020 SALZBURG, Austria/Europe Phone work: +43+662+4482+4720 Mobile phone work:+43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss") E-Mail work: W.Muss-at-SALK.at
------------------------------------------------------------------------ ---- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (familia_zepeda-at-yahoo.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, October 20, 2005 at 22:15:54 ---------------------------------------------------------------------------
Question: I am doing a research project on William Nichol who invented the polarizing microscope, but i cant seem to find any biographical information on him is there anyway you can help me?
William Nicol invented the 'Nicol prism' in 1828. This polarizer prism is "a rhombohedren of calcite, cut diagonally, ground and polished and cemented together with Canada balsam". However not many living microscopists will have ever used one, as Edwin Land subsequently invented 'Polaroid' plastic sheet in 1932 and this is now always used in place of the Nicol prism as the polarizer/analyser.
Regards
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
Tel: 020 7608 4050 Fax: 020 7608 4034 email: keith.morris-at-ucl.ac.uk ----- Original Message ----- X-from: {familia_zepeda-at-yahoo.com} To: {keith.morris-at-ucl.ac.uk} Sent: Friday, October 21, 2005 2:05 PM
Hey...Watch it.
My first pol scope had nicol polarized and cap analyzer. I'm still using that scope as the balsam hasn't separated. Oh, yes it has a mirror and I use a focusable lamp. I used to do dispersion staining for asbestos and microchemical test with that scope... Boy, thinking of it bring back memories....
Frank Karl Degussa Corporation Akron Technical Center 3500 Embassy Parkway Suite 100 Akron, Ohio 44333
330-668-2235 Ext. 238
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keith.morris-at-ucl. ac.uk To: frank.karl-at-degussa.com cc: 10/21/2005 10:08 Subject: [Microscopy] Re: AskAMicroscopist:inventor of polorizing microscope AM Please respond to keith.morris
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear Irene,
William Nicol invented the 'Nicol prism' in 1828. This polarizer prism is "a rhombohedren of calcite, cut diagonally, ground and polished and cemented together with Canada balsam". However not many living microscopists will have ever used one, as Edwin Land subsequently invented 'Polaroid' plastic sheet in 1932 and this is now always used in place of the Nicol prism as the polarizer/analyser.
Regards
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
Tel: 020 7608 4050 Fax: 020 7608 4034 email: keith.morris-at-ucl.ac.uk ----- Original Message ----- X-from: {familia_zepeda-at-yahoo.com} To: {keith.morris-at-ucl.ac.uk} Sent: Friday, October 21, 2005 2:05 PM
Greetings, I think that the Nicol type prism provides high quality extinction, better than the Land type. I expect there have been modern refinements to the design since Nicol but a pair of cemented appropriately cut crystals provide excellent extinction. I believe they are common on optical benches in physics applications -- it can be awkward working the prisms into a microscope light path.
As ever, Tobias
} } } } Hey...Watch it. } } My first pol scope had nicol polarized and cap analyzer. I'm still using } that scope as the balsam hasn't separated. Oh, yes it has a mirror and I } use a focusable lamp. I used to do dispersion staining for asbestos and } microchemical test with that scope... Boy, thinking of it bring back } memories.... } } Frank Karl } Degussa Corporation } Akron Technical Center } 3500 Embassy Parkway } Suite 100 } Akron, Ohio 44333 } } 330-668-2235 Ext. 238 } } } } } } keith.morris-at-ucl. } ac.uk To: } frank.karl-at-degussa.com } } cc: } 10/21/2005 10:08 Subject: } [Microscopy] Re: AskAMicroscopist:inventor of polorizing } microscope } } AM } Please respond } to } keith.morris } } } } } } Dear Irene, } } William Nicol invented the 'Nicol prism' in 1828. This polarizer prism is } "a } rhombohedren of calcite, cut diagonally, ground and polished and cemented } together with Canada balsam". However not many living microscopists will } have ever used one, as Edwin Land subsequently invented 'Polaroid' plastic } sheet in 1932 and this is now always used in place of the Nicol prism as } the } polarizer/analyser. } } Regards } } Keith } } ---------------------------------------------------------- } Dr Keith J Morris } Imaging Facilities Manager } Cell Biology Division } Institute of Ophthalmology } University College London } 11-43 Bath Street } London EC1V 9EL } }
Another problem with the plastic polarizing filters is that they fade quickly or melt outright under intense light sources, e.g. a Xenon lamp. I wonder if the cement used in the cut crystal prisms would also experience a problem with high light intensity. BTW, I found that heat filters did not sufficiently help; neutral density filters did help but those defeated the purpose of the high intensity lamp we were trying to use.
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Greetings, I think that the Nicol type prism provides high quality extinction, better than the Land type. I expect there have been modern refinements to the design since Nicol but a pair of cemented appropriately cut crystals provide excellent extinction. I believe they are common on optical benches in physics applications -- it can be awkward working the prisms into a microscope light path.
As ever, Tobias
==============================Original Headers============================== 9, 23 -- From neuberger1234-at-comcast.net Fri Oct 21 12:28:25 2005 9, 23 -- Received: from rwcrmhc12.comcast.net (rwcrmhc14.comcast.net [204.127.198.54]) 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9LHSPNc019556 9, 23 -- for {microscopy-at-microscopy.com} ; Fri, 21 Oct 2005 12:28:25 -0500 9, 23 -- Received: from personal73sg05 (c-67-167-236-68.hsd1.il.comcast.net[67.167.236.68]) 9, 23 -- by comcast.net (rwcrmhc14) with SMTP 9, 23 -- id {200510211728240140066urve} ; Fri, 21 Oct 2005 17:28:24 +0000 9, 23 -- From: "Damian Neuberger" {neuberger1234-at-comcast.net} 9, 23 -- To: {baskin-at-bio.umass.edu} 9, 23 -- Cc: "Microscopy-at-Microscopy. Com" {microscopy-at-microscopy.com} 9, 23 -- Subject: RE: [Microscopy] Re: AskAMicroscopist:inventor of polorizing 9, 23 -- Date: Fri, 21 Oct 2005 12:28:25 -0500 9, 23 -- Message-ID: {OKEJIDCNBDPPLLHANALIAEBNDCAA.neuberger1234-at-comcast.net} 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="iso-8859-1" 9, 23 -- Content-Transfer-Encoding: 7bit 9, 23 -- X-Priority: 3 (Normal) 9, 23 -- X-MSMail-Priority: Normal 9, 23 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.6604 (9.0.2911.0) 9, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 9, 23 -- In-Reply-To: {200510211635.j9LGZgQo010413-at-ns.microscopy.com} 9, 23 -- Importance: Normal ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (woad-at-iinet.net.au) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Friday, October 21, 2005 at 12:16:15 ---------------------------------------------------------------------------
Title-Subject: [Filtered] 3d phase contrast light microscopy
Question: I remember running across a particular university group that had at one stage been working on generating 3d image from phase contrast LM images. However I can't find the link as I've forgotten it...does anybody know of any people/links/papers doing work in this area?
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Couple weeks ago, I posted a question on whether it is necessary to have enough (or any) ethanol to cover the sample in the CPD chamber at the beginning of CPD. I received many replies and are grateful for the advice. Here is a summary of the replies.
1. Filling the CPD chamber with ethanol is not necessary. In some cases, there are enough ethanol in the CPD chamber and the specimen basket that evaporation is minimal, if any. Some preparations are more tolerant to this condition.
2. Filling the CPD chamber with ethanol is essential. The majority of the replies recommends never letting the preparation exposed to air during dehydration to avoid uneven evaporation, which will defeat the purpose of doing CPD. Delicate specimens can probably benefit from a more cautious approach.
3. One reply suggest using amyl acetate as the intermediate fluid after ethanol dehydration, but I don't think I will use that because the need of access to a fume hood. So far, ethanol and CO2 alone has given us good results. At least for those preps that we were able to compare to live specimens.
I will continue to suggest to users of our facility to be aware of the condition mentioned in #2 above and hopefully the user will adopt the appropriate practice. For my own preps, I will definitely go with #2. Thanks again for sharing your expertise. Cheers.
-- Pang (Wai Pang Chan, wpchan-at-u.washington.edu)
==============================Original Headers============================== 6, 19 -- From wpchan-at-u.washington.edu Mon Oct 24 01:43:26 2005 6, 19 -- Received: from mxout1.cac.washington.edu (mxout1.cac.washington.edu [140.142.32.134]) 6, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9O6hQSA020229 6, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Oct 2005 01:43:26 -0500 6, 19 -- Received: from homer21.u.washington.edu (homer21.u.washington.edu [140.142.12.133]) 6, 19 -- by mxout1.cac.washington.edu (8.13.4+UW05.04/8.13.4+UW05.09) with ESMTP id j9O6hPq6029517 6, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 19 -- for {Microscopy-at-microscopy.com} ; Sun, 23 Oct 2005 23:43:25 -0700 6, 19 -- Received: from localhost (wpchan-at-localhost) 6, 19 -- by homer21.u.washington.edu (8.13.4+UW05.04/8.13.4+Submit) with ESMTP id j9O6hPiA000678 6, 19 -- for {Microscopy-at-microscopy.com} ; Sun, 23 Oct 2005 23:43:25 -0700 6, 19 -- Date: Sun, 23 Oct 2005 23:43:25 -0700 (PDT) 6, 19 -- From: "W. Chan" {wpchan-at-u.washington.edu} 6, 19 -- To: Microscopy-at-microscopy.com 6, 19 -- Subject: replies to Re: CPD 6, 19 -- Message-ID: {Pine.LNX.4.63a.0510101015270.28356-at-homer23.u.washington.edu} 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 6, 19 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
I cannot access ReindeerGraphics.com this morning (publishers of Fovea Pro image analysis plugin suite for Photoshop). Does anyone have information as to the status of the web site? Perhaps someone has an 800 or other area code number for their offices? Any help appreciated.
Thanks, Bill
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==============================Original Headers============================== 10, 22 -- From William.H.Roberts-at-USA.dupont.com Mon Oct 24 11:15:35 2005 10, 22 -- Received: from mms02bas.mms.us.syntegra.com (relay2.mms.us.syntegra.com [150.143.103.20]) 10, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9OGFZlF004249 10, 22 -- for {microscopy-at-microscopy.com} ; Mon, 24 Oct 2005 11:15:35 -0500 10, 22 -- Received: from mms01es.mms.us.syntegra.com (mms01es.mms.us.syntegra.com [150.143.104.10]) by mms02bas.mms.us.syntegra.com with ESMTP for microscopy-at-microscopy.com; Mon, 24 Oct 2005 16:15:34 Z 10, 22 -- Received: from mms03bas.mms.us.syntegra.com (mms03bas.mms.us.syntegra.com [150.143.103.50]) by mms01es.mms.us.syntegra.com with ESMTP for microscopy-at-microscopy.com; Mon, 24 Oct 2005 16:15:34 Z 10, 22 -- Received: from demhub21.lvs.dupont.com (demhub21.lvs.dupont.com [52.128.29.204]) by mms03bas.mms.us.syntegra.com with ESMTP for microscopy-at-microscopy.com; Mon, 24 Oct 2005 16:15:33 Z 10, 22 -- Received: from unknown (HELO demhub2.lvs.dupont.com) (52.99.10.48) 10, 22 -- by demhub21.lvs.dupont.com with ESMTP; 24 Oct 2005 12:15:33 -0400 10, 22 -- X-DUPONT-INTERNET: Recieved via DuPont Internet Mail Relay 10, 22 -- Received: from cdcln05.lvs.dupont.com (52.99.26.10) 10, 22 -- by demhub2.lvs.dupont.com with ESMTP; 24 Oct 2005 12:15:33 -0400 10, 22 -- From: William H Roberts {William.H.Roberts-at-USA.dupont.com} 10, 22 -- Subject: ReindeerGraphics.com 10, 22 -- To: microscopy-at-microscopy.com 10, 22 -- X-Mailer: Lotus Notes Release 6.0.3 September 26, 2003 10, 22 -- Message-Id: {OF76E69B37.0AF6A4CD-ON852570A4.0059018E-852570A4.00594EB3-at-CDCLN05.LVS.DUPONT.COM} 10, 22 -- Date: Mon, 24 Oct 2005 12:15:28 -0400 10, 22 -- X-MIMETrack: Serialize by Router on CDCLNMH1/DuPont_MHUB(Release 6.0.5|March 10, 22 -- 27, 2005) at 10/24/2005 12:15:33 PM 10, 22 -- MIME-Version: 1.0 10, 22 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
} Dear Listers, } } I cannot access ReindeerGraphics.com this morning (publishers of Fovea Pro } image analysis plugin suite for Photoshop). Does anyone have information } as to the status of the web site? Perhaps someone has an 800 or other } area code number for their offices? Any help appreciated. } } Thanks, Bill
You can reach the Reindeer Graphics offices at 919.342.0209. RGI's website is hosted in Boca Raton, FL, which probably gives you some hint as to why its not up right now.
B
P.S. Please forward this to the Microscopy list - I can't seem to send to them right now. Thanks! -- Brent Neal Geek of all Trades "Specialization is for insects" -- Robert A. Heinlein http://brentn.freeshell.org
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==============================Original Headers============================== 19, 22 -- From William.H.Roberts-at-USA.dupont.com Mon Oct 24 11:44:41 2005 19, 22 -- Received: from mms21bas.mms.us.syntegra.com (relay21.mms.us.syntegra.com [192.12.251.10]) 19, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9OGif8l013603 19, 22 -- for {microscopy-at-microscopy.com} ; Mon, 24 Oct 2005 11:44:41 -0500 19, 22 -- Received: from mms23es.mms.us.syntegra.com (mms23es.mms.us.syntegra.com [150.143.232.50]) by mms21bas.mms.us.syntegra.com with ESMTP for microscopy-at-microscopy.com; Mon, 24 Oct 2005 16:44:40 Z 19, 22 -- Received: from mms21bas.mms.us.syntegra.com (mms21bas.mms.us.syntegra.com [192.12.251.10]) by mms23es.mms.us.syntegra.com with ESMTP for microscopy-at-microscopy.com; Mon, 24 Oct 2005 16:44:35 Z 19, 22 -- Received: from demhub21.lvs.dupont.com (demhub21.lvs.dupont.com [52.128.29.204]) by mms21bas.mms.us.syntegra.com with ESMTP for microscopy-at-microscopy.com; Mon, 24 Oct 2005 16:44:35 Z 19, 22 -- Received: from unknown (HELO demhub1.lvs.dupont.com) (52.99.10.45) 19, 22 -- by demhub21.lvs.dupont.com with ESMTP; 24 Oct 2005 12:44:35 -0400 19, 22 -- X-DUPONT-INTERNET: Recieved via DuPont Internet Mail Relay 19, 22 -- Received: from cdcln05.lvs.dupont.com (52.99.26.10) 19, 22 -- by demhub1.lvs.dupont.com with ESMTP; 24 Oct 2005 12:44:34 -0400 19, 22 -- From: William H Roberts {William.H.Roberts-at-USA.dupont.com} 19, 22 -- Subject: Fw: [Microscopy] ReindeerGraphics.com 19, 22 -- To: microscopy-at-microscopy.com 19, 22 -- X-Mailer: Lotus Notes Release 6.0.3 September 26, 2003 19, 22 -- Message-Id: {OFA07DAE87.2C2BBE33-ON852570A4.005BAB1D-852570A4.005BB875-at-CDCLN05.LVS.DUPONT.COM} 19, 22 -- Date: Mon, 24 Oct 2005 12:41:50 -0400 19, 22 -- X-MIMETrack: Serialize by Router on CDCLNMH1/DuPont_MHUB(Release 6.0.5|March 19, 22 -- 27, 2005) at 10/24/2005 12:44:34 PM 19, 22 -- MIME-Version: 1.0 19, 22 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
} Dear Listers, } } I cannot access ReindeerGraphics.com this morning (publishers of Fovea Pro } image analysis plugin suite for Photoshop). Does anyone have information } as to the status of the web site? Perhaps someone has an 800 or other } area code number for their offices? Any help appreciated. } } Thanks, Bill
You can reach the Reindeer Graphics offices at 919.342.0209. RGI's website is hosted in Boca Raton, FL, which probably gives you some hint as to why its not up right now.
B
P.S. Please forward this to the Microscopy list - I can't seem to send to them right now. Thanks! -- Brent Neal Geek of all Trades "Specialization is for insects" -- Robert A. Heinlein http://brentn.freeshell.org
==============================Original Headers============================== 11, 21 -- From neuberger1234-at-comcast.net Mon Oct 24 11:57:07 2005 11, 21 -- Received: from sccrmhc11.comcast.net (sccrmhc11.comcast.net [204.127.202.55]) 11, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9OGv63U022376 11, 21 -- for {microscopy-at-microscopy.com} ; Mon, 24 Oct 2005 11:57:06 -0500 11, 21 -- Received: from personal73sg05 (c-67-167-236-68.hsd1.il.comcast.net[67.167.236.68]) 11, 21 -- by comcast.net (sccrmhc11) with SMTP 11, 21 -- id {2005102416570501100s0cene} ; Mon, 24 Oct 2005 16:57:05 +0000 11, 21 -- From: "Damian Neuberger" {neuberger1234-at-comcast.net} 11, 21 -- To: "Microscopy-at-Microscopy. Com" {microscopy-at-microscopy.com} 11, 21 -- Subject: Fw: ReindeerGraphics.com 11, 21 -- Date: Mon, 24 Oct 2005 11:57:03 -0500 11, 21 -- Message-ID: {OKEJIDCNBDPPLLHANALIAEDEDCAA.neuberger1234-at-comcast.net} 11, 21 -- MIME-Version: 1.0 11, 21 -- Content-Type: text/plain; 11, 21 -- charset="iso-8859-1" 11, 21 -- Content-Transfer-Encoding: 7bit 11, 21 -- X-Priority: 3 (Normal) 11, 21 -- X-MSMail-Priority: Normal 11, 21 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.6604 (9.0.2911.0) 11, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 11, 21 -- Importance: Normal ==============================End of - Headers==============================
Look up the UNESCO guides on oceanographic methodology. They have one specifically on zooplankton fixation and preservation. Also, I have this and another guide or two at home, still in boxes. I'll try to remember to bring them in so I can the references. But ... what are you trying to fix and to analyze? If labile ions like Na, K, Ca and the like, you really need to use cryomethods for microanalysis. If the elements you're after are not labile, then standard Karnovsky's and so on may work.
Phil
Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
----------
Hi,
Can any of you provide me a reference or procedure for preparing zooplankton for TEM + microanalysis?
Thanks!
Owen Owen P. Mills Michigan Technological University Rm 512 M&M Bldg. Houghton, MI 49931 PH 906-369-1875 FAX 906-487-2934 mailto:opmills-at-mtu.edu http://www.mm.mtu.edu/~opmills
==============================Original Headers============================== 7, 31 -- From opmills-at-mtu.edu Mon Oct 24 15:01:56 2005 7, 31 -- Received: from node8.mtu.edu (node8.mtu.edu [141.219.69.8]) 7, 31 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9OK1uqp001231 7, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Oct 2005 15:01:56 -0500 7, 31 -- Received: from node5.edge.dcsint.mtu.edu (node5.mtu.edu [141.219.69.5]) 7, 31 -- by node8.mtu.edu (8.13.1/8.13.1) with ESMTP id j9OK1uWn022674 7, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Oct 2005 16:01:56 -0400 7, 31 -- Received: from node34.edge.dcsint.mtu.edu (node34.mtu.edu [141.219.69.34]) 7, 31 -- by node5.edge.dcsint.mtu.edu (8.12.10/8.12.8) with ESMTP id j9OK1uvh032580 7, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Oct 2005 16:01:56 -0400 7, 31 -- Received: from mail.mtu.edu (campus4.mtu.edu [141.219.70.7]) 7, 31 -- by node34.edge.dcsint.mtu.edu (8.12.11/8.12.11) with ESMTP id j9OK1s4j007955 7, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Oct 2005 16:01:55 -0400 7, 31 -- (envelope-from opmills-at-mtu.edu) 7, 31 -- Received: from node6.edge.dcsint.mtu.edu (node6.mtu.edu [141.219.69.6]) 7, 31 -- by mail.mtu.edu (8.11.7p1+Sun/8.11.6) with ESMTP id j9OK1st14491 7, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Oct 2005 16:01:54 -0400 (EDT) 7, 31 -- Received: from [141.219.192.45] (opmills.eecn.sabo.mtu.edu [141.219.192.45]) 7, 31 -- by node6.edge.dcsint.mtu.edu (8.12.10/8.12.3/auth_ssl.mc v1.0) with ESMTP id j9OK1sH0030320 7, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Oct 2005 16:01:54 -0400 7, 31 -- Mime-Version: 1.0 (Apple Message framework v734) 7, 31 -- Content-Transfer-Encoding: 7bit 7, 31 -- Message-Id: {4A1145FE-7868-4996-9B14-CFCF12271B8F-at-mtu.edu} 7, 31 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 31 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 31 -- From: "Owen P. Mills" {opmills-at-mtu.edu} 7, 31 -- Subject: [MICROSCOPY]TEM preparation of zooplankton 7, 31 -- Date: Mon, 24 Oct 2005 16:05:30 -0400 7, 31 -- X-Mailer: Apple Mail (2.734) 7, 31 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.0.3.2, Antispam-Data: 2005.10.24.24 7, 31 -- X-PerlMx-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
==============================Original Headers============================== 14, 30 -- From oshel1pe-at-cmich.edu Mon Oct 24 15:16:28 2005 14, 30 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 14, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9OKGSWF010005 14, 30 -- for {microscopy-at-microscopy.com} ; Mon, 24 Oct 2005 15:16:28 -0500 14, 30 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 14, 30 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id j9OKxswi006889 14, 30 -- for {microscopy-at-microscopy.com} ; Mon, 24 Oct 2005 16:59:54 -0400 14, 30 -- Received: from cmail4.central.cmich.local ([141.209.15.84]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 14, 30 -- Mon, 24 Oct 2005 16:16:31 -0400 14, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 14, 30 -- Content-class: urn:content-classes:message 14, 30 -- MIME-Version: 1.0 14, 30 -- Content-Type: text/plain; 14, 30 -- charset="iso-8859-1" 14, 30 -- Subject: RE: [Microscopy] TEM preparation of zooplankton 14, 30 -- Date: Mon, 24 Oct 2005 16:11:40 -0400 14, 30 -- Message-ID: {07BD45BB3EE8A0468C94776E7A0C660E072F24-at-cmail4.central.cmich.local} 14, 30 -- X-MS-Has-Attach: 14, 30 -- X-MS-TNEF-Correlator: 14, 30 -- Thread-Topic: [Microscopy] TEM preparation of zooplankton 14, 30 -- Thread-Index: AcXY1vXWKu+79V6JQtSPZjJq81jK1gAAC8xJ 14, 30 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 14, 30 -- To: {opmills-at-mtu.edu} 14, 30 -- Cc: {microscopy-at-microscopy.com} 14, 30 -- X-OriginalArrivalTime: 24 Oct 2005 20:16:31.0625 (UTC) FILETIME=[D2CE9790:01C5D8D7] 14, 30 -- X-CanItPRO-Stream: default 14, 30 -- X-Spam-Score: 0.8 () J_CHICKENPOX_57,TW_UQ,TW_UV 14, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 14, 30 -- Content-Transfer-Encoding: 8bit 14, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9OKGSWF010005 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (eeloe-at-ucsd.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, October 24, 2005 at 17:34:46 ---------------------------------------------------------------------------
Email: eeloe-at-ucsd.edu Name: Emiley Eloe
Organization: Scripps Institution of Oceanography
Education: Graduate College
Location: La Jolla, CA USA
Question: I'm trying to prepare Vibrio parahaemolyticus samples for SEM on 2216-marine agar plates and am having difficulties visualizing the bacterial cells on the agar surface. I have used a 1% glut/0.1M cacodylic acid plus 3.5% salt fixative and fixed cells by gently pouring over the agar plate. It appears as though cells are somehow diffusing or migrating into the agar and avoiding being fixed directly on the surface. Any help would be appreciated.
Carefully cut out 1 millimeter X ~10 mm X ~1 mm pieces of the agar where the bacteria are. Fix for a couple of hours at room temperature (or overnight in the 'frig) and dehydrate as usual. After the final 100% EtOH step, drop the pieces into liquid nitrogen. The EtOH freezes vitreously, and so won't cause any freezing artifacts. If the pieces don't spontaneously fall apart, break them with a razor blade. This will expose the bacteria in the agar. (This method also works for all sorts of things.) Return to 100% EtOH, then critical-point dry.
Phil
Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
-----Original Message----- X-from: eeloe-at-ucsd.edu [mailto:eeloe-at-ucsd.edu] Sent: Mon 24-Oct-05 19:21 To: Oshel, Philip Eugene
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (eeloe-at-ucsd.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, October 24, 2005 at 17:34:46 ---------------------------------------------------------------------------
Email: eeloe-at-ucsd.edu Name: Emiley Eloe
Organization: Scripps Institution of Oceanography
Education: Graduate College
Location: La Jolla, CA USA
Question: I'm trying to prepare Vibrio parahaemolyticus samples for SEM on 2216-marine agar plates and am having difficulties visualizing the bacterial cells on the agar surface. I have used a 1% glut/0.1M cacodylic acid plus 3.5% salt fixative and fixed cells by gently pouring over the agar plate. It appears as though cells are somehow diffusing or migrating into the agar and avoiding being fixed directly on the surface. Any help would be appreciated.
==============================Original Headers============================== 16, 30 -- From oshel1pe-at-cmich.edu Tue Oct 25 07:27:36 2005 16, 30 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 16, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9PCRaIo008120 16, 30 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2005 07:27:36 -0500 16, 30 -- Received: from egateb.central.cmich.local ([141.209.15.85]) 16, 30 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id j9PDB0wi017521 16, 30 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2005 09:11:00 -0400 16, 30 -- Received: from cmail4.central.cmich.local ([141.209.15.84]) by egateb.central.cmich.local with Microsoft SMTPSVC(5.0.2195.6713); 16, 30 -- Tue, 25 Oct 2005 08:27:25 -0400 16, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 16, 30 -- Content-class: urn:content-classes:message 16, 30 -- MIME-Version: 1.0 16, 30 -- Content-Type: text/plain; 16, 30 -- charset="iso-8859-1" 16, 30 -- Subject: RE: [Microscopy] AskAMicroscopist: Vibrio parahaemolyticus samples for SEM 16, 30 -- Date: Tue, 25 Oct 2005 08:23:12 -0400 16, 30 -- Message-ID: {07BD45BB3EE8A0468C94776E7A0C660E072F29-at-cmail4.central.cmich.local} 16, 30 -- X-MS-Has-Attach: 16, 30 -- X-MS-TNEF-Correlator: 16, 30 -- Thread-Topic: [Microscopy] AskAMicroscopist: Vibrio parahaemolyticus samples for SEM 16, 30 -- Thread-Index: AcXY8bXFEUrf3Si7TAiKuyKwFUC9xgAbSgJv 16, 30 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 16, 30 -- To: {eeloe-at-ucsd.edu} 16, 30 -- Cc: {microscopy-at-microscopy.com} 16, 30 -- X-OriginalArrivalTime: 25 Oct 2005 12:27:25.0963 (UTC) FILETIME=[751925B0:01C5D95F] 16, 30 -- X-CanItPRO-Stream: default 16, 30 -- X-Spam-Score: 0 () 16, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 16, 30 -- Content-Transfer-Encoding: 8bit 16, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9PCRaIo008120 ==============================End of - Headers==============================
A couple of months back I asked the listserver about preparing porous samples for TEM, and the advice I received worked quite well. For sake of the archival information, I wanted to comment on what suggestions were made, which were applied and the results of the work. This may be common knowledge for some, but it may be of some assistance to others in the future. Any suggestions or comments are also welcomed such that they will be included in the thread for future searches.
Preparing TEM samples from sintered powders of metals or oxides Problem: Due to the large amount of porosity in certain sintered powder samples, polishing or dimpling to a thickness of 10-15 um is difficult because the sample has very little mechanical strength and is susceptible to crack formation even at small forces.
Proposed Solutions: Infiltrate with crazy glue or sulfur to fill in the porosity, thereby making the sample more homogeneous. The rigidity of the infiltrated media will assist in reducing the cracks formed within the sample during polishing or dimpling.
Steps Taken: Samples were polished down to 75-100 um and then placed in pure acetone to remove any crystal bond from the pores. While crystal bond did partially fill in the pores, it was not rigid enough to keep the sample intact at final polishing steps. The samples may need to be polished down farther before they are infiltrated with crazy glue depending on the extent of the porosity. Typically though, if the crazy glue does not fully penetrate the sample at 75-100 um, then the sample is probably compact enough to polish to 10-15 um without infiltration.
Once removed from acetone, drops of crazy glue (store bought) were placed on the sample until it appeared that it had stopped absorbing the glue. The sample was then drawn across a piece of wax paper until the bottom of the sample did not stick to the wax paper. This was done to ensure that the sample remains flat so that when it is remounted it is not uneven. The sample was then left to cure for the recommended time and temperature for crazy glue (denoted on package).
A second, more ingenious, method was developed by a co-worker with similar issues in that he cut a plastic capsule to the shape of a small tube and stuck it down to double sided tape on a glass slide. The sample was stuck inside the tube onto the tape (keeping a flat base), and crazy glue was subsequently poured into the tube covering the sample. The crazy glue was cured at the appropriate time and temperature.
After curing, the samples were then polished down to ~10-15 um, mounted on Cu slot grids and subsequently ion milled.
Addendum: Morphologically (e.g., the thickness of a thin anodized layer or shape of a sintered particulate), the presence of a polymer layer during ion milling is definitely beneficial because it provides a watermark for the oxide layer. That is to say, one can be assured that the edge of an oxidized layer or particle is true, and has not been milled away during preparation. Yet, for spectroscopy, the samples we were analyzing required the absence of a carbon signal. The presence of the crazy glue during ion milling tended to give a thin film of redeposited carbon on the samples, thereby confounding our data analysis. In this case, we infiltrated with crazy glue in one of the aforementioned manners, polished the sample to ~10-15 um, affixed them to Cu slot grids and finally put them through a series of solution baths. As prescribed by an older email on the listserver, the sample was bathed in pure acetone for 10 minutes, followed by a pure ethanol bath for 10 minutes and finally bathed in pure (as pure as possible in one's lab) water for 10 minutes. Between each bath the sample was allowed to dry. The acetone removes the remnants of the crazy glue. The ethanol helps to remove any remaining acetone residue, and finally the water helps to remove any ethanol residue.
Results: After plasma cleaning the samples for 10 minutes (in the case of the bathed samples), the reduction of C signal in our samples was greatly reduced if not eliminated. In order to do STEM/EELS, a cold stage was used to further reduce any carbon contamination from the microscope. As previously mentioned, when the crazy glue was left intact during ion milling, the morphology of the anodized thin films could easily be observed.
Regards Matt Olszta Department of Materials Science and Engineering Penn State University
==============================Original Headers============================== 11, 16 -- From mjo10-at-psu.edu Tue Oct 25 15:03:01 2005 11, 16 -- Received: from f04n01.cac.psu.edu (f04s01.cac.psu.edu [128.118.141.31]) 11, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9PK30uC023816 11, 16 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Oct 2005 15:03:01 -0500 11, 16 -- Received: from Matt.psu.edu (olszta.mri.psu.edu [146.186.179.10]) 11, 16 -- by f04n01.cac.psu.edu (8.13.2/8.13.2) with ESMTP id j9PK2xB7050822 11, 16 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Oct 2005 16:02:59 -0400 11, 16 -- Message-Id: {6.2.3.4.2.20051025160036.01fba138-at-email.psu.edu} 11, 16 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 11, 16 -- Date: Tue, 25 Oct 2005 16:01:04 -0400 11, 16 -- To: Microscopy-at-microscopy.com 11, 16 -- From: Matthew Olszta {mjo10-at-psu.edu} 11, 16 -- Subject: Preparing Porous Sintered TEM Samples 11, 16 -- Mime-Version: 1.0 11, 16 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 11, 16 -- X-Virus-Scanned: by amavisd-new ==============================End of - Headers==============================
I would like to mount a small webcam that I've adapted for NIR imaging in one eyepiece of a Nikon SMZ-10 microscope. I'm using a 23mm eyepiece, and the scope eyepiece is a 30mm size. Does anyone know of an inexpensive source of an adapter ring?
Henry Barwood Associate Professor of Science, Earth Science Department of Math and Physics MSCX 312G Troy University Troy, Alabama 36082 hbarwood-at-troy.edu
==============================Original Headers============================== 3, 25 -- From hbarwood-at-troy.edu Tue Oct 25 15:14:37 2005 3, 25 -- Received: from webshield01.troy.edu (scan.troy.edu [198.179.130.124]) 3, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9PKEbUF032538 3, 25 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2005 15:14:37 -0500 3, 25 -- Received: from (198.179.130.118) by webshield01.troy.edu via smtp 3, 25 -- id 0b46_3160e4be_4595_11da_85b3_0002b3cdc1aa; 3, 25 -- Tue, 25 Oct 2005 20:23:23 +0000 (UTC) 3, 25 -- Received: from amy ([10.10.5.248]) 3, 25 -- by mail.troy.edu (MOS 3.5.6-GR) 3, 25 -- with ESMTP id BGF08037 (AUTH hbarwood); 3, 25 -- Tue, 25 Oct 2005 15:13:26 -0500 (CDT) 3, 25 -- From: "Henry Barwood" {hbarwood-at-troy.edu} 3, 25 -- To: "Microscopy-at-Microscopy. Com" {microscopy-at-microscopy.com} 3, 25 -- Subject: 23mm to 30mm eyepiece adapter 3, 25 -- Date: Tue, 25 Oct 2005 15:16:42 -0500 3, 25 -- Message-ID: {NFBBLIEMIMFMNHKCOKOCGEPNFCAA.hbarwood-at-troy.edu} 3, 25 -- MIME-Version: 1.0 3, 25 -- Content-Type: text/plain; 3, 25 -- charset="iso-8859-1" 3, 25 -- Content-Transfer-Encoding: 7bit 3, 25 -- X-Priority: 3 (Normal) 3, 25 -- X-MSMail-Priority: Normal 3, 25 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0) 3, 25 -- Importance: Normal 3, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1409 ==============================End of - Headers==============================
I recently received a copy of the Zeiss Innovation magazine which focuses on Ernst Abbe's contributions to microscopy. In addition to some interesting history, there are nice explanations of NA, immersion fluids and magnification. The back of the book is typical ad material but the front is worth a read. if you didn't get one, ask your zeiss rep or email them. i have no financial interest in zeiss - i am just a user (sometimes happy, sometimes not) of the product. Tom Phillips
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
A lab user is having problems with ice crystals growing on cryo-grids during transfers to the microscope.
Room humidity varies, but is typically 50%. Any tricks for reducing it and ice crystal growth?
I thought about a room dehumidifier, but the ones I see say they only go down to 30% and I don't know how effective it would be to try to do the whole room.
Some kind of chamber with silica gel or ??.
Thanks
Jon
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-cats.ucsc.edu
==============================Original Headers============================== 10, 21 -- From jmkrupp-at-cats.ucsc.edu Tue Oct 25 18:56:15 2005 10, 21 -- Received: from cats-mx4.ucsc.edu (cats-mx4.ucsc.edu [128.114.125.37]) 10, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9PNuFp1020530 10, 21 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2005 18:56:15 -0500 10, 21 -- Received: from [128.114.25.168] (dhcp-25-168.ucsc.edu [128.114.25.168]) 10, 21 -- by cats-mx4.ucsc.edu (8.13.1/8.13.1) with SMTP id j9PNsfcE018031 10, 21 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2005 16:54:45 -0700 (PDT) 10, 21 -- X-Sender: jmkrupp-at-cruzmail.ucsc.edu 10, 21 -- Message-Id: {v01550107bf84744caf61-at-[128.114.25.168]} 10, 21 -- Mime-Version: 1.0 10, 21 -- Content-Type: text/plain; charset="us-ascii" 10, 21 -- Date: Tue, 25 Oct 2005 16:50:52 -0700 10, 21 -- To: microscopy-at-microscopy.com 10, 21 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp) 10, 21 -- Subject: how to dehumidify? 10, 21 -- X-UCSC-CATS-Information: This message was scanned by the ITS MailScanner 10, 21 -- X-UCSC-CATS-MailScanner: Found to be clean 10, 21 -- X-UCSC-CATS-MailScanner-SpamCheck: not spam, spamassassin (score=1.57, 10, 21 -- required 8, autolearn=disabled, MISSING_SUBJECT 1.57) 10, 21 -- X-UCSC-CATS-MailScanner-SpamScore: s 10, 21 -- X-UCSC-CATS-MailScanner-From: jmkrupp-at-cats.ucsc.edu ==============================End of - Headers==============================
Jonathon, There is an easy way to do this. Get a garbage bag and run a Tygon(R) tube from a cylinder of nitrogen or from bleed off from liquid nitrogen. Put your bag over your sample and put the tube into the bag and flush the volume with nitrogen. Alternatively, you can use or newly introduced Thing-A-Ma-Jug(TM) which can make a positive pressure of N2 or CO2 from the head space of a Dewar of liquid nitrogen or dry ice. Transport your sample under the bag with the nitrogen to the TEM and work under the bag. (Note, I said under the bag, not in the bag.) I think that you can keep the humidity under the bag close to 0%.
If you use a high flow of N2, please make sure that you have adequate ventilation.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu] Sent: Tuesday, October 25, 2005 5:02 PM To: Walck-at-SouthBayTech.com
Hi:
A lab user is having problems with ice crystals growing on cryo-grids during transfers to the microscope.
Room humidity varies, but is typically 50%. Any tricks for reducing it and ice crystal growth?
I thought about a room dehumidifier, but the ones I see say they only go down to 30% and I don't know how effective it would be to try to do the whole room.
Some kind of chamber with silica gel or ??.
Thanks
Jon
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-cats.ucsc.edu
==============================Original Headers============================== 10, 21 -- From jmkrupp-at-cats.ucsc.edu Tue Oct 25 18:56:15 2005 10, 21 -- Received: from cats-mx4.ucsc.edu (cats-mx4.ucsc.edu [128.114.125.37]) 10, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9PNuFp1020530 10, 21 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2005 18:56:15 -0500 10, 21 -- Received: from [128.114.25.168] (dhcp-25-168.ucsc.edu [128.114.25.168]) 10, 21 -- by cats-mx4.ucsc.edu (8.13.1/8.13.1) with SMTP id j9PNsfcE018031 10, 21 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2005 16:54:45 -0700 (PDT) 10, 21 -- X-Sender: jmkrupp-at-cruzmail.ucsc.edu 10, 21 -- Message-Id: {v01550107bf84744caf61-at-[128.114.25.168]} 10, 21 -- Mime-Version: 1.0 10, 21 -- Content-Type: text/plain; charset="us-ascii" 10, 21 -- Date: Tue, 25 Oct 2005 16:50:52 -0700 10, 21 -- To: microscopy-at-microscopy.com 10, 21 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp) 10, 21 -- Subject: how to dehumidify? 10, 21 -- X-UCSC-CATS-Information: This message was scanned by the ITS MailScanner 10, 21 -- X-UCSC-CATS-MailScanner: Found to be clean 10, 21 -- X-UCSC-CATS-MailScanner-SpamCheck: not spam, spamassassin (score=1.57, 10, 21 -- required 8, autolearn=disabled, MISSING_SUBJECT 1.57) 10, 21 -- X-UCSC-CATS-MailScanner-SpamScore: s 10, 21 -- X-UCSC-CATS-MailScanner-From: jmkrupp-at-cats.ucsc.edu ==============================End of - Headers==============================
==============================Original Headers============================== 21, 24 -- From walck-at-southbaytech.com Tue Oct 25 21:00:28 2005 21, 24 -- Received: from pimout6-ext.prodigy.net (pimout6-ext.prodigy.net [207.115.63.78]) 21, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9Q20S4f030495 21, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Oct 2005 21:00:28 -0500 21, 24 -- X-ORBL: [64.169.193.90] 21, 24 -- Received: from dynamicbl8uno3 (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90]) 21, 24 -- by pimout6-ext.prodigy.net (8.13.4 outbound domainkey aix/8.13.4) with ESMTP id j9Q1xqAS142516; 21, 24 -- Tue, 25 Oct 2005 21:59:57 -0400 21, 24 -- From: "Scott Walck" {walck-at-southbaytech.com} 21, 24 -- To: {Microscopy-at-microscopy.com} 21, 24 -- Cc: {jmkrupp-at-cats.ucsc.edu} 21, 24 -- Subject: RE: [Microscopy] how to dehumidify? 21, 24 -- Date: Tue, 25 Oct 2005 18:59:58 -0700 21, 24 -- Message-ID: {004001c5d9d0$fdf712e0$7801a8c0-at-dynamicbl8uno3} 21, 24 -- MIME-Version: 1.0 21, 24 -- Content-Type: text/plain; 21, 24 -- charset="US-ASCII" 21, 24 -- Content-Transfer-Encoding: 7bit 21, 24 -- X-Priority: 3 (Normal) 21, 24 -- X-MSMail-Priority: Normal 21, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 21, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 21, 24 -- In-Reply-To: {200510260001.j9Q01xE5027192-at-ns.microscopy.com} 21, 24 -- Importance: Normal ==============================End of - Headers==============================
jmkrupp-at-cats.ucsc.edu wrote: } Hi: } } A lab user is having problems with ice crystals growing on cryo-grids } during transfers to the microscope. } } Room humidity varies, but is typically 50%. Any tricks for reducing it and } ice crystal growth? } } I thought about a room dehumidifier, but the ones I see say they only go } down to 30% and I don't know how effective it would be to try to do the } whole room. } } Some kind of chamber with silica gel or ??. } } Thanks } } Jon } Put a dehumidifier or large boxes of dehumidifying agent than you can regenerate with heat in a plastic tent over his work station or even a smaller tent around the scope. The humidly is much easier and cheaper to control in a small space and two make an airlock design so other experiments in the room don't mess with yours.
In cold rooms the space is usually filled with work sooner than later.
Gordon Gordon Couger
I collect links on information related to light microscopes. www.couger.com/microscope/links/gclinks.html Please forward anything you think might be useful to others. Microscope Documentation is at www.science-info.org
==============================Original Headers============================== 8, 21 -- From gcc-at-couger.com Tue Oct 25 22:35:21 2005 8, 21 -- Received: from centrmmtao01.cox.net (centrmmtao01.cox.net [70.168.83.83]) 8, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9Q3ZLdN008106 8, 21 -- for {microscopy-at-msa.microscopy.com} ; Tue, 25 Oct 2005 22:35:21 -0500 8, 21 -- Received: from [127.0.0.1] (really [68.12.242.13]) by centrmmtao01.cox.net 8, 21 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 8, 21 -- id {20051026033502.TCPG5336.centrmmtao01.cox.net-at-[127.0.0.1]} ; 8, 21 -- Tue, 25 Oct 2005 23:35:02 -0400 8, 21 -- Message-ID: {435EF980.40603-at-couger.com} 8, 21 -- Date: Tue, 25 Oct 2005 22:35:28 -0500 8, 21 -- From: Gordon Couger {gcc-at-couger.com} 8, 21 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 8, 21 -- X-Accept-Language: en-us, en 8, 21 -- MIME-Version: 1.0 8, 21 -- To: jmkrupp-at-cats.ucsc.edu, 8, 21 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} 8, 21 -- Subject: Re: [Microscopy] how to dehumidify? 8, 21 -- References: {200510260000.j9Q002og025246-at-ns.microscopy.com} 8, 21 -- In-Reply-To: {200510260000.j9Q002og025246-at-ns.microscopy.com} 8, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
What software is currently available, commercial or freeware, that will generate single-crystal selected-area diffraction patterns for a given crystal for a given zone axis, given its cell parameters and atomic positions etc? The Desktop Microscopist product used to do this, but it seems to have disappeared from the landscape in the intervening last few years that I've been away from microscopy (can't find it on Google). I remember that most HRTEM multislice image simulation programs will generate SA or CBED patterns, but I'm looking for something a little simpler.
Thanks,
Roy Christoffersen SAIC Code KA-Astromaterials Directorate NASA Johnson Space Center
==============================Original Headers============================== 5, 19 -- From rcsaic-at-sbcglobal.net Wed Oct 26 09:17:17 2005 5, 19 -- Received: from smtp111.sbc.mail.mud.yahoo.com (smtp111.sbc.mail.mud.yahoo.com [68.142.198.210]) 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j9QEHHjo025216 5, 19 -- for {Microscopy-at-Microscopy.Com} ; Wed, 26 Oct 2005 09:17:17 -0500 5, 19 -- Message-Id: {200510261417.j9QEHHjo025216-at-ns.microscopy.com} 5, 19 -- Received: (qmail 65879 invoked from network); 26 Oct 2005 14:17:17 -0000 5, 19 -- Received: from unknown (HELO STUDYDESKTOP) (rcsaic-at-sbcglobal.net-at-70.240.176.147 with login) 5, 19 -- by smtp111.sbc.mail.mud.yahoo.com with SMTP; 26 Oct 2005 14:17:17 -0000 5, 19 -- From: "Roy Christoffersen" {rcsaic-at-sbcglobal.net} 5, 19 -- To: "Microscopy Listserver" {Microscopy-at-Microscopy.Com} 5, 19 -- Subject: General TEM: Software for generating SAD patterns 5, 19 -- Date: Wed, 26 Oct 2005 09:17:19 -0500 5, 19 -- MIME-Version: 1.0 5, 19 -- Content-Type: text/plain; 5, 19 -- charset="US-ASCII" 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 5, 19 -- Thread-Index: AcXaN5iA8oT2YtPzTdGW6e2qMRa7vg== 5, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1409 ==============================End of - Headers==============================
The advice from Dr. Oshel does not solve the problem that the bacteria would not stay together during fixation in aqueous glutaraldehyde. When I wanted to see the surface of a colony, I fixed the small pieces of the agar gel with the colonies on them in osmium tetroxide vapour in a humid chamber and then carefully dehydrated them in a graded ethanol series.
If the original writer only wants to get images of the bacteria irrespective whether they are from the top or the bottom of the colony, it would be advisable to gently release (from a pasteur pipette) a thin layer of } 40°C 3-4% agar sol. (I used to incorporate glutaraldehyde into the agar sol before use). This coating would immobilize the bacteria in the colony and the sample would be dehydrated and critical-point dried as usual. The dried agar coating would be opened easily before mounting the bacteria on a stub.
You may provide this kind of advice and you do not need to mention my name. Regards,
Milos
Note: Milos is a retired scientist.
Ann Fook
-----Original Message----- X-from: Yang, Ann-Fook Sent: Wednesday, October 26, 2005 9:54 AM To: Kalab, Miloslav
Carefully cut out 1 millimeter X ~10 mm X ~1 mm pieces of the agar where the bacteria are. Fix for a couple of hours at room temperature (or overnight in the 'frig) and dehydrate as usual. After the final 100% EtOH step, drop the pieces into liquid nitrogen. The EtOH freezes vitreously, and so won't cause any freezing artifacts. If the pieces don't spontaneously fall apart, break them with a razor blade. This will expose the bacteria in the agar. (This method also works for all sorts of things.) Return to 100% EtOH, then critical-point dry.
Phil
Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
-----Original Message----- X-from: eeloe-at-ucsd.edu [mailto:eeloe-at-ucsd.edu] Sent: Mon 24-Oct-05 19:21 To: Oshel, Philip Eugene
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (eeloe-at-ucsd.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, October 24, 2005 at 17:34:46 ---------------------------------------------------------------------------
Email: eeloe-at-ucsd.edu Name: Emiley Eloe
Organization: Scripps Institution of Oceanography
Education: Graduate College
Location: La Jolla, CA USA
Question: I'm trying to prepare Vibrio parahaemolyticus samples for SEM on 2216-marine agar plates and am having difficulties visualizing the bacterial cells on the agar surface. I have used a 1% glut/0.1M cacodylic acid plus 3.5% salt fixative and fixed cells by gently pouring over the agar plate. It appears as though cells are somehow diffusing or migrating into the agar and avoiding being fixed directly on the surface. Any help would be appreciated.
==============================Original Headers============================== 16, 30 -- From oshel1pe-at-cmich.edu Tue Oct 25 07:27:36 2005 16, 30 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 16, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9PCRaIo008120 16, 30 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2005 07:27:36 -0500 16, 30 -- Received: from egateb.central.cmich.local ([141.209.15.85]) 16, 30 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id j9PDB0wi017521 16, 30 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2005 09:11:00 -0400 16, 30 -- Received: from cmail4.central.cmich.local ([141.209.15.84]) by egateb.central.cmich.local with Microsoft SMTPSVC(5.0.2195.6713); 16, 30 -- Tue, 25 Oct 2005 08:27:25 -0400 16, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 16, 30 -- Content-class: urn:content-classes:message 16, 30 -- MIME-Version: 1.0 16, 30 -- Content-Type: text/plain; 16, 30 -- charset="iso-8859-1" 16, 30 -- Subject: RE: [Microscopy] AskAMicroscopist: Vibrio parahaemolyticus samples for SEM 16, 30 -- Date: Tue, 25 Oct 2005 08:23:12 -0400 16, 30 -- Message-ID: {07BD45BB3EE8A0468C94776E7A0C660E072F29-at-cmail4.central.cmich.local} 16, 30 -- X-MS-Has-Attach: 16, 30 -- X-MS-TNEF-Correlator: 16, 30 -- Thread-Topic: [Microscopy] AskAMicroscopist: Vibrio parahaemolyticus samples for SEM 16, 30 -- Thread-Index: AcXY8bXFEUrf3Si7TAiKuyKwFUC9xgAbSgJv 16, 30 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 16, 30 -- To: {eeloe-at-ucsd.edu} 16, 30 -- Cc: {microscopy-at-microscopy.com} 16, 30 -- X-OriginalArrivalTime: 25 Oct 2005 12:27:25.0963 (UTC) FILETIME=[751925B0:01C5D95F] 16, 30 -- X-CanItPRO-Stream: default 16, 30 -- X-Spam-Score: 0 () 16, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 16, 30 -- Content-Transfer-Encoding: 8bit 16, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9PCRaIo008120 ==============================End of - Headers==============================
==============================Original Headers============================== 35, 29 -- From YANGA-at-AGR.GC.CA Wed Oct 26 09:21:37 2005 35, 29 -- Received: from agroutsmtp1.agr.gc.ca (agroutsmtp1.agr.gc.ca [192.197.71.175]) 35, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9QELbv7028118 35, 29 -- for {microscopy-at-microscopy.com} ; Wed, 26 Oct 2005 09:21:37 -0500 35, 29 -- Received: from agrin1-old.agr.gc.ca (agrgate.agr.ca [192.197.71.189]) 35, 29 -- by agroutsmtp1.agr.gc.ca (8.12.8/8.12.8) with ESMTP id j9QELYCD028616 35, 29 -- for {microscopy-at-microscopy.com} ; Wed, 26 Oct 2005 10:21:35 -0400 35, 29 -- Received: from onncrxcn1.AGR.GC.CA ([10.117.15.130]) 35, 29 -- by agrin1-old.agr.gc.ca (8.12.8/8.12.8) with ESMTP id j9QEMDbn026148 35, 29 -- for {microscopy-at-microscopy.com} ; Wed, 26 Oct 2005 10:22:25 -0400 35, 29 -- Received: from onncrxms3.AGR.GC.CA ([10.117.15.30]) by onncrxcn1.AGR.GC.CA with Microsoft SMTPSVC(5.0.2195.6713); 35, 29 -- Wed, 26 Oct 2005 10:20:57 -0400 35, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 35, 29 -- content-class: urn:content-classes:message 35, 29 -- MIME-Version: 1.0 35, 29 -- Content-Type: text/plain; 35, 29 -- charset="iso-8859-1" 35, 29 -- Subject: FW: [Microscopy] RE: AskAMicroscopist: Vibrio parahaemolyticus samples for SEM 35, 29 -- Date: Wed, 26 Oct 2005 10:18:05 -0400 35, 29 -- Message-ID: {E035A9C87303AE4AB9BA10FD8324DFB1355441-at-onncrxms3.agr.gc.ca} 35, 29 -- X-MS-Has-Attach: 35, 29 -- X-MS-TNEF-Correlator: 35, 29 -- Thread-Topic: [Microscopy] RE: AskAMicroscopist: Vibrio parahaemolyticus samples for SEM 35, 29 -- Thread-Index: AcXZYD9p4xQtTXWxR/GJTtnBdZ1t/gA1FdUwAAAnC4AAAIIncA== 35, 29 -- From: "Yang, Ann-Fook" {YANGA-at-AGR.GC.CA} 35, 29 -- To: {microscopy-at-microscopy.com} 35, 29 -- X-OriginalArrivalTime: 26 Oct 2005 14:20:57.0027 (UTC) FILETIME=[7B38C930:01C5DA38] 35, 29 -- Content-Transfer-Encoding: 8bit 35, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9QELbv7028118 ==============================End of - Headers==============================
It won't? Odd. I've used the fix-EtOH cryofracture methods many times to study bacteria and yeast within (and on) agar without the critters "not stay[ing] together". The glutaraldehyde fixation step fixes the bacteria in place quite nicely. Phil
Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
The advice from Dr. Oshel does not solve the problem that the bacteria would not stay together during fixation in aqueous glutaraldehyde. When I wanted to see the surface of a colony, I fixed the small pieces of the agar gel with the colonies on them in osmium tetroxide vapour in a humid chamber and then carefully dehydrated them in a graded ethanol series.
If the original writer only wants to get images of the bacteria irrespective whether they are from the top or the bottom of the colony, it would be advisable to gently release (from a pasteur pipette) a thin layer of } 40°C 3-4% agar sol. (I used to incorporate glutaraldehyde into the agar sol before use). This coating would immobilize the bacteria in the colony and the sample would be dehydrated and critical-point dried as usual. The dried agar coating would be opened easily before mounting the bacteria on a stub.
You may provide this kind of advice and you do not need to mention my name. Regards,
Milos
Note: Milos is a retired scientist.
Ann Fook
-----Original Message----- X-from: Yang, Ann-Fook Sent: Wednesday, October 26, 2005 9:54 AM To: Kalab, Miloslav
Carefully cut out 1 millimeter X ~10 mm X ~1 mm pieces of the agar where the bacteria are. Fix for a couple of hours at room temperature (or overnight in the 'frig) and dehydrate as usual. After the final 100% EtOH step, drop the pieces into liquid nitrogen. The EtOH freezes vitreously, and so won't cause any freezing artifacts. If the pieces don't spontaneously fall apart, break them with a razor blade. This will expose the bacteria in the agar. (This method also works for all sorts of things.) Return to 100% EtOH, then critical-point dry.
Phil
Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
-----Original Message----- X-from: eeloe-at-ucsd.edu [mailto:eeloe-at-ucsd.edu] Sent: Mon 24-Oct-05 19:21 To: Oshel, Philip Eugene
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (eeloe-at-ucsd.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, October 24, 2005 at 17:34:46 ---------------------------------------------------------------------------
Email: eeloe-at-ucsd.edu Name: Emiley Eloe
Organization: Scripps Institution of Oceanography
Education: Graduate College
Location: La Jolla, CA USA
Question: I'm trying to prepare Vibrio parahaemolyticus samples for SEM on 2216-marine agar plates and am having difficulties visualizing the bacterial cells on the agar surface. I have used a 1% glut/0.1M cacodylic acid plus 3.5% salt fixative and fixed cells by gently pouring over the agar plate. It appears as though cells are somehow diffusing or migrating into the agar and avoiding being fixed directly on the surface. Any help would be appreciated.
==============================Original Headers============================== 16, 30 -- From oshel1pe-at-cmich.edu Tue Oct 25 07:27:36 2005 16, 30 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 16, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9PCRaIo008120 16, 30 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2005 07:27:36 -0500 16, 30 -- Received: from egateb.central.cmich.local ([141.209.15.85]) 16, 30 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id j9PDB0wi017521 16, 30 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2005 09:11:00 -0400 16, 30 -- Received: from cmail4.central.cmich.local ([141.209.15.84]) by egateb.central.cmich.local with Microsoft SMTPSVC(5.0.2195.6713); 16, 30 -- Tue, 25 Oct 2005 08:27:25 -0400 16, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 16, 30 -- Content-class: urn:content-classes:message 16, 30 -- MIME-Version: 1.0 16, 30 -- Content-Type: text/plain; 16, 30 -- charset="iso-8859-1" 16, 30 -- Subject: RE: [Microscopy] AskAMicroscopist: Vibrio parahaemolyticus samples for SEM 16, 30 -- Date: Tue, 25 Oct 2005 08:23:12 -0400 16, 30 -- Message-ID: {07BD45BB3EE8A0468C94776E7A0C660E072F29-at-cmail4.central.cmich.local} 16, 30 -- X-MS-Has-Attach: 16, 30 -- X-MS-TNEF-Correlator: 16, 30 -- Thread-Topic: [Microscopy] AskAMicroscopist: Vibrio parahaemolyticus samples for SEM 16, 30 -- Thread-Index: AcXY8bXFEUrf3Si7TAiKuyKwFUC9xgAbSgJv 16, 30 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 16, 30 -- To: {eeloe-at-ucsd.edu} 16, 30 -- Cc: {microscopy-at-microscopy.com} 16, 30 -- X-OriginalArrivalTime: 25 Oct 2005 12:27:25.0963 (UTC) FILETIME=[751925B0:01C5D95F] 16, 30 -- X-CanItPRO-Stream: default 16, 30 -- X-Spam-Score: 0 () 16, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 16, 30 -- Content-Transfer-Encoding: 8bit 16, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9PCRaIo008120 ==============================End of - Headers==============================
==============================Original Headers============================== 35, 29 -- From YANGA-at-AGR.GC.CA Wed Oct 26 09:21:37 2005 35, 29 -- Received: from agroutsmtp1.agr.gc.ca (agroutsmtp1.agr.gc.ca [192.197.71.175]) 35, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9QELbv7028118 35, 29 -- for {microscopy-at-microscopy.com} ; Wed, 26 Oct 2005 09:21:37 -0500 35, 29 -- Received: from agrin1-old.agr.gc.ca (agrgate.agr.ca [192.197.71.189]) 35, 29 -- by agroutsmtp1.agr.gc.ca (8.12.8/8.12.8) with ESMTP id j9QELYCD028616 35, 29 -- for {microscopy-at-microscopy.com} ; Wed, 26 Oct 2005 10:21:35 -0400 35, 29 -- Received: from onncrxcn1.AGR.GC.CA ([10.117.15.130]) 35, 29 -- by agrin1-old.agr.gc.ca (8.12.8/8.12.8) with ESMTP id j9QEMDbn026148 35, 29 -- for {microscopy-at-microscopy.com} ; Wed, 26 Oct 2005 10:22:25 -0400 35, 29 -- Received: from onncrxms3.AGR.GC.CA ([10.117.15.30]) by onncrxcn1.AGR.GC.CA with Microsoft SMTPSVC(5.0.2195.6713); 35, 29 -- Wed, 26 Oct 2005 10:20:57 -0400 35, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 35, 29 -- content-class: urn:content-classes:message 35, 29 -- MIME-Version: 1.0 35, 29 -- Content-Type: text/plain; 35, 29 -- charset="iso-8859-1" 35, 29 -- Subject: FW: [Microscopy] RE: AskAMicroscopist: Vibrio parahaemolyticus samples for SEM 35, 29 -- Date: Wed, 26 Oct 2005 10:18:05 -0400 35, 29 -- Message-ID: {E035A9C87303AE4AB9BA10FD8324DFB1355441-at-onncrxms3.agr.gc.ca} 35, 29 -- X-MS-Has-Attach: 35, 29 -- X-MS-TNEF-Correlator: 35, 29 -- Thread-Topic: [Microscopy] RE: AskAMicroscopist: Vibrio parahaemolyticus samples for SEM 35, 29 -- Thread-Index: AcXZYD9p4xQtTXWxR/GJTtnBdZ1t/gA1FdUwAAAnC4AAAIIncA== 35, 29 -- From: "Yang, Ann-Fook" {YANGA-at-AGR.GC.CA} 35, 29 -- To: {microscopy-at-microscopy.com} 35, 29 -- X-OriginalArrivalTime: 26 Oct 2005 14:20:57.0027 (UTC) FILETIME=[7B38C930:01C5DA38] 35, 29 -- Content-Transfer-Encoding: 8bit 35, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9QELbv7028118 ==============================End of - Headers==============================
==============================Original Headers============================== 37, 30 -- From oshel1pe-at-cmich.edu Wed Oct 26 11:04:03 2005 37, 30 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 37, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9QG43ES011372 37, 30 -- for {microscopy-at-microscopy.com} ; Wed, 26 Oct 2005 11:04:03 -0500 37, 30 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 37, 30 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id j9QGlOwi030925 37, 30 -- for {microscopy-at-microscopy.com} ; Wed, 26 Oct 2005 12:47:24 -0400 37, 30 -- Received: from cmail4.central.cmich.local ([141.209.15.84]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 37, 30 -- Wed, 26 Oct 2005 12:04:07 -0400 37, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 37, 30 -- Content-class: urn:content-classes:message 37, 30 -- MIME-Version: 1.0 37, 30 -- Content-Type: text/plain; 37, 30 -- charset="iso-8859-1" 37, 30 -- Subject: RE: [Microscopy] FW: RE: AskAMicroscopist: Vibrio parahaemolyticus samples for SEM 37, 30 -- Date: Wed, 26 Oct 2005 12:00:48 -0400 37, 30 -- Message-ID: {07BD45BB3EE8A0468C94776E7A0C660E072F4E-at-cmail4.central.cmich.local} 37, 30 -- X-MS-Has-Attach: 37, 30 -- X-MS-TNEF-Correlator: 37, 30 -- Thread-Topic: [Microscopy] FW: RE: AskAMicroscopist: Vibrio parahaemolyticus samples for SEM 37, 30 -- Thread-Index: AcXaOc3yIOZ1p0TKSNqltYgi4HfhmAADKCTy 37, 30 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 37, 30 -- To: {YANGA-at-AGR.GC.CA} 37, 30 -- Cc: {microscopy-at-microscopy.com} 37, 30 -- X-OriginalArrivalTime: 26 Oct 2005 16:04:07.0907 (UTC) FILETIME=[E5461B30:01C5DA46] 37, 30 -- X-CanItPRO-Stream: default 37, 30 -- X-Spam-Score: 0 () 37, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 37, 30 -- Content-Transfer-Encoding: 8bit 37, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9QG43ES011372 ==============================End of - Headers==============================
Asylum Research, a premier manufacturer of atomic force microscopes, has two positions available for East Coast Technical Sales. Information can be found http://www.asylumresearch.com/About/ About.shtml#CR
Terry Mehr Asylum Research www.AsylumResearch.com
==============================Original Headers============================== 2, 13 -- From terry-at-AsylumResearch.com Wed Oct 26 13:08:17 2005 2, 13 -- Received: from exchange.AsylumResearch.com (net-cf9a4f83.cst.impulse.net [207.154.79.131]) 2, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9QI8Hts021332 2, 13 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Oct 2005 13:08:17 -0500 2, 13 -- Mime-Version: 1.0 (Apple Message framework v734) 2, 13 -- Content-Transfer-Encoding: 7bit 2, 13 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 2, 13 -- To: Microscopy-at-microscopy.com 2, 13 -- From: Terry Mehr {terry-at-AsylumResearch.com} 2, 13 -- Subject: AFM Technical Sales Positions Available 2, 13 -- Date: Wed, 26 Oct 2005 11:08:15 -0700 2, 13 -- X-Mailer: Apple Mail (2.734) 2, 13 -- Message-ID: {1EUphA-0001ck-B2-at-exchange.AsylumResearch.com} ==============================End of - Headers==============================
} I have a Wild M7A stereomicroscope and the image(s) are edged } with yellow on one side and, if you close one eye, blue on } the other. This causes a yellow cast to object edges and is } very annoying. } } I am using either x20 or x10 eyepieces, with LED or f-optic } ring illumination. The problem is most obvious under higher } magnifications. } } Does anyone know why this happens, and how it can be solved?
I expect I will get hate mail from Wild fans, but I think you are seeing chromatic aberration. There is nothing you can do about it, except buy a better microscope.
I am not familiar with the M7A, but in my last job I used several Wild M5 stereos. One of them showed yellow/blue aberration, and the others showed red/green aberration. They were all regularly serviced by Leitz.
The Zeiss stereos used by the other entomologists, and the Olympus SZ-III stereos used for training courses, did not show any obvious chromatic aberration.
-- Alan Wood http://www.alanwood.net (Unicode, special characters, pesticide names)
==============================Original Headers============================== 6, 16 -- From alan.wood-at-JUSTIS.COM Thu Oct 27 08:04:37 2005 6, 16 -- Received: from smarthost1.mail.uk.easynet.net (smarthost1.mail.uk.easynet.net [212.135.6.11]) 6, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9RD4bba024732 6, 16 -- for {Microscopy-at-microscopy.com} ; Thu, 27 Oct 2005 08:04:37 -0500 6, 16 -- Received: from [195.40.43.130] (helo=mail.mail) 6, 16 -- by smarthost1.mail.uk.easynet.net with esmtp (Exim 4.10) 6, 16 -- id 1EV7Qq-000I7p-00; Thu, 27 Oct 2005 14:04:36 +0100 6, 16 -- Message-ID: {404B9B1EBAD25A4ABC1BE9C20B2DF12401B5B54D-at-mail.mail} 6, 16 -- From: Alan Wood {alan.wood-at-JUSTIS.COM} 6, 16 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 6, 16 -- Cc: "'curt-at-oxfordenvironment.co.uk'" {curt-at-oxfordenvironment.co.uk} 6, 16 -- Subject: RE: [Microscopy] viaWWW: M7A Yellow blue edging on image 6, 16 -- Date: Thu, 27 Oct 2005 14:02:13 +0100 6, 16 -- MIME-Version: 1.0 6, 16 -- Content-Type: text/plain; 6, 16 -- charset="iso-8859-1" ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kjl226-at-vt.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, October 26, 2005 at 09:49:15 ---------------------------------------------------------------------------
Email: kjl226-at-vt.edu Name: Kathy lowe
Organization: Virginia Tech, College of Vet. Medicine
Title-Subject: [Filtered] MListserver: Protcol for embedding whole mosquitos
Question: Does anyone have a procedure for processing and embedding whole mosquitos for TEM? Kathy Lowe
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (RW-at-web.de) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, October 27, 2005 at 05:24:16 ---------------------------------------------------------------------------
Email: RW-at-web.de Name: Ramona Wesselman
Organization: Center for Nanotchnology
Education: Graduate College
Location: Rheine, germany
Question: Hallo, I am working with Yeast, I want to know if you treid to use Citifluor for embeding alive yeastcells, or if you can give me an advice for another embeding medium which is possible for yeast cells and has an index of refraction of 1,46 . Thanks!
I'm curious to see if anyone has any words of wisdom about immunostaining of GFP in general. I'd be interested in experiences relating to DAB-HRP labeling and/or immunogold, both pre- and post-embedding. Cryosections, too! (There, that ought to keep you busy.)
More specifically, are there any danger spots in the fixation or embedding processes that could negatively affect the labeling process, or even destroy the protein itself?
I know it's one of those overly broad questions, but I am mainly interested in how, if at all, labeling GFP might be different that labeling other proteins. I've not had much luck going through our library on finding GFP-specific material, and I'm still Googling away.
By the way, you're all invited to dinner at my place tonight by way of thanks. My Honduran in-laws are in town and the food is good and plentiful and the music is Latin. Yes, people CAN cook and dance at the same time.
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 10, 23 -- From TindallR-at-missouri.edu Thu Oct 27 12:43:35 2005 10, 23 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49]) 10, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9RHhZAB030929 10, 23 -- for {microscopy-at-microscopy.com} ; Thu, 27 Oct 2005 12:43:35 -0500 10, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 10, 23 -- Thu, 27 Oct 2005 12:43:34 -0500 10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 10, 23 -- Content-class: urn:content-classes:message 10, 23 -- MIME-Version: 1.0 10, 23 -- Content-Type: text/plain; 10, 23 -- charset="us-ascii" 10, 23 -- Subject: Immuno EM---GFP 10, 23 -- Date: Thu, 27 Oct 2005 12:43:34 -0500 10, 23 -- Message-ID: {BA876152E8653240BE8572E897083EE79804BC-at-UM-EMAIL09.um.umsystem.edu} 10, 23 -- X-MS-Has-Attach: 10, 23 -- X-MS-TNEF-Correlator: 10, 23 -- Thread-Topic: Immuno EM---GFP 10, 23 -- Thread-Index: AcXbHfQFbshrz551RZu1z6r4WMdnPg== 10, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 10, 23 -- To: {microscopy-at-microscopy.com} 10, 23 -- X-OriginalArrivalTime: 27 Oct 2005 17:43:34.0876 (UTC) FILETIME=[F44711C0:01C5DB1D] 10, 23 -- Content-Transfer-Encoding: 8bit 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9RHhZAB030929 ==============================End of - Headers==============================
Randy - Check out Grabenbauer et al (1005) Nature Methods 2(11):857- Correlative microscopy and electron tomography of GFP through photooxidation - they use GFP bleaching to drive DAB oxidation into an electron dense precipitate.
I am old enough to have done a fair amount of DAB immunolabeling at the EM level. It is much less satisfying than colloidal gold. So maybe a gold method would be better in some circumstances.
GFP survives PF but not ethanol.
And while I believe that people can dance and cook at the same time, I have yet to see you dance whenever I have been at your home. Tom
At 12:46 PM 10/27/05, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
We are looking for a device for maintaining a constant temperature inside a plexiglass incubator which is installed around the stage of an inverted microscope. Volume around 2 cu. ft.
We now have an external heating unit with temperature control that blows warm air into the incubator, however the air then flows out through small openings and it is difficult to regulate the CO2 levels because the air is constantly changing. Besides, the warm air results in fluid evaporation.
Ideally, I'm looking for a radiant source of heat to place inside the incubator - no light bulbs, for obvious reasons. We do have a thermistor to regulate the temperature into which the device could be plugged.
Any suggestions? Thank you Judy
Judy Trogadis Bio-Imaging Coordinator St. Michael's Hospital, 7Queen 30 Bond St. Toronto, ON M5B 1W8 Canada ph: 416-864-6060 x6337 pager: 416-685-9219 fax: 416-864-6043 trogadisj-at-smh.toronto.on.ca
==============================Original Headers============================== 7, 19 -- From trogadisj-at-smh.toronto.on.ca Thu Oct 27 16:09:45 2005 7, 19 -- Received: from smh.toronto.on.ca (mail.smh.toronto.on.ca [199.71.175.103]) 7, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9RL9jt7027278 7, 19 -- for {microscopy-at-msa.microscopy.com} ; Thu, 27 Oct 2005 16:09:45 -0500 7, 19 -- Received: from ([199.71.171.15]) 7, 19 -- by beethoven.smh.toronto.on.ca with ESMTP id KP-GCT47.5438033; 7, 19 -- Thu, 27 Oct 2005 17:09:20 -0400 7, 19 -- Received: from SMTPOUT-MTA by beethoven.smh.toronto.on.ca 7, 19 -- with Novell_GroupWise; Thu, 27 Oct 2005 17:09:20 -0400 7, 19 -- Message-Id: {s36109bf.006-at-beethoven.smh.toronto.on.ca} 7, 19 -- X-Mailer: Novell GroupWise Internet Agent 6.5.2 7, 19 -- Date: Thu, 27 Oct 2005 17:08:54 -0400 7, 19 -- From: "Judy Trogadis" {TrogadisJ-at-smh.toronto.on.ca} 7, 19 -- To: {Microscopy-at-msa.microscopy.com} 7, 19 -- Subject: heating live cells 7, 19 -- Mime-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=US-ASCII 7, 19 -- Content-Transfer-Encoding: 7bit 7, 19 -- Content-Disposition: inline ==============================End of - Headers==============================
Dear Randy, Haven't done immunolabelling on tissues or sections, but the molecular biologists here use anti-GFP and standard procedures to detect their labelled proteins on westerns. These are not native gels, just standard SDS-PAGE gels blotted onto nitrocellulose membrane, so the protein is denatured. cheers, Rosemary
Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 02-6246 5475 GPO Box 1600 mob. 0402 835 973 Canberra, ACT 2601 fax. 02-6246 5334 Australia
} From: TindallR-at-missouri.edu } Reply-To: TindallR-at-missouri.edu } Date: Thu, 27 Oct 2005 12:50:03 -0500 } To: rosemary.white-at-csiro.au } Subject: [Microscopy] Immuno EM---GFP } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } I'm curious to see if anyone has any words of wisdom about } immunostaining of GFP in general. I'd be interested in experiences } relating to DAB-HRP labeling and/or immunogold, both pre- and } post-embedding. Cryosections, too! (There, that ought to keep you } busy.) } } More specifically, are there any danger spots in the fixation or } embedding processes that could negatively affect the labeling process, } or even destroy the protein itself? } } I know it's one of those overly broad questions, but I am mainly } interested in how, if at all, labeling GFP might be different that } labeling other proteins. I've not had much luck going through our } library on finding GFP-specific material, and I'm still Googling away. } } By the way, you're all invited to dinner at my place tonight by way of } thanks. My Honduran in-laws are in town and the food is good and } plentiful and the music is Latin. Yes, people CAN cook and dance at the } same time. } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W122 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } } } } } } } } ==============================Original Headers============================== } 10, 23 -- From TindallR-at-missouri.edu Thu Oct 27 12:43:35 2005 } 10, 23 -- Received: from um-exproto9.um.umsystem.edu } (um-exproto9.um.umsystem.edu [207.160.151.49]) } 10, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9RHhZAB030929 } 10, 23 -- for {microscopy-at-microscopy.com} ; Thu, 27 Oct 2005 12:43:35 -0500 } 10, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by } um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); } 10, 23 -- Thu, 27 Oct 2005 12:43:34 -0500 } 10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 } 10, 23 -- Content-class: urn:content-classes:message } 10, 23 -- MIME-Version: 1.0 } 10, 23 -- Content-Type: text/plain; } 10, 23 -- charset="us-ascii" } 10, 23 -- Subject: Immuno EM---GFP } 10, 23 -- Date: Thu, 27 Oct 2005 12:43:34 -0500 } 10, 23 -- Message-ID: } {BA876152E8653240BE8572E897083EE79804BC-at-UM-EMAIL09.um.umsystem.edu} } 10, 23 -- X-MS-Has-Attach: } 10, 23 -- X-MS-TNEF-Correlator: } 10, 23 -- Thread-Topic: Immuno EM---GFP } 10, 23 -- Thread-Index: AcXbHfQFbshrz551RZu1z6r4WMdnPg== } 10, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 10, 23 -- To: {microscopy-at-microscopy.com} } 10, 23 -- X-OriginalArrivalTime: 27 Oct 2005 17:43:34.0876 (UTC) } FILETIME=[F44711C0:01C5DB1D] } 10, 23 -- Content-Transfer-Encoding: 8bit } 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id j9RHhZAB030929 } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 21 -- From Rosemary.White-at-csiro.au Thu Oct 27 16:56:11 2005 4, 21 -- Received: from act-ironport-ext-out1.csiro.au (act-ironport-ext-out1.csiro.au [150.229.7.37]) 4, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9RLuAOk004072 4, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 27 Oct 2005 16:56:11 -0500 4, 21 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 4, 21 -- by act-ironport-ext-out1.csiro.au with ESMTP; 28 Oct 2005 07:56:08 +1000 4, 21 -- X-IronPort-AV: i="3.97,259,1125842400"; 4, 21 -- d="scan'208"; a="67291287:sNHT25168676" 4, 21 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 4, 21 -- Fri, 28 Oct 2005 07:56:07 +1000 4, 21 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 4, 21 -- Date: Fri, 28 Oct 2005 07:57:40 +1000 4, 21 -- Subject: Re: [Microscopy] Immuno EM---GFP 4, 21 -- From: Rosemary White {Rosemary.White-at-csiro.au} 4, 21 -- To: {TindallR-at-missouri.edu} 4, 21 -- CC: {Microscopy-at-microscopy.com} 4, 21 -- Message-ID: {BF878A74.12C0C%Rosemary.White-at-csiro.au} 4, 21 -- Mime-version: 1.0 4, 21 -- Content-type: text/plain; charset="US-ASCII" 4, 21 -- Content-transfer-encoding: 7bit 4, 21 -- X-OriginalArrivalTime: 27 Oct 2005 21:56:07.0734 (UTC) FILETIME=[3C15B560:01C5DB41] ==============================End of - Headers==============================
I haven't seen any replies to your question on the Microscopy Listserver, so I thought I'd ask a biologist friend of mine about what procedure scientists use to look at cancer cells under the microscope. Of course, someone (probably much more knowledgable than I) may have replied to you off-server.
Anyway, to detect cancer, pathologists use light (optical) microscopes to look at slices of tissue on glass slides stained to show internal cell organelles, and sometimes relationships of cells to other cells.
Sometimes, they may examine suspensions of blood or bone marrow cells spread on glass slides and stained to detect unusual features. These doctors are experts in knowing what normal tissues and cells look like and can detect unusual features, such as enlarged and or strangely convoluted nuclei, unusually long and numerous microvilli, or cells invading tissue where they should not be located.
Additionally, they may order special stains to detect kinds of antigens that are not found on normal tissue or that show up in unusual locations, for example, whether breast or prostate tissue is found in some other organ. Some pathologists and researchers use the electron microscope to detect ultrafine structure (structure smaller than you see with an optical microscope) inside cells -- such as premelanosomes, desmosomes, or secretory granules -- to identify different types of tumor cells. It is very important for the doctors to know the kind of cancer is present because often different types respond to different therapies.
Other experts doing research on tissues and cell cultures may use special forms of optical microscopy such as fluorescence or confocal microscopy to detect the presence of particular structures or locate special antigens in cells. Researchers can contribute to the knowledge of how cancer cells live and therefore what kinds of agents might be used to kill them.
I hope that helps.
Mike O'Keefe MSA Director
----- Original Message ----- X-from: loz_jay01-at-hotmail.com
Hi
Anyone like to offer an opinion of the differences in image quality between LD and standard objective lenses for light microscopy including fluorescence?
We have a 'hybrid' scope, some LD, some standard objectives and are trying to decide which kind to add to go to make them all the same.
Pretty general question, I know, but give it a shot if you have some ideas.
Thanks
Jon
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-cats.ucsc.edu
==============================Original Headers============================== 10, 21 -- From jmkrupp-at-cats.ucsc.edu Thu Oct 27 19:06:28 2005 10, 21 -- Received: from cats-mx1.ucsc.edu (cats-mx1.ucsc.edu [128.114.125.34]) 10, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9S06RLq022715 10, 21 -- for {microscopy-at-microscopy.com} ; Thu, 27 Oct 2005 19:06:28 -0500 10, 21 -- Received: from [128.114.25.209] (dhcp-25-209.ucsc.edu [128.114.25.209]) 10, 21 -- by cats-mx1.ucsc.edu (8.13.1/8.13.1) with SMTP id j9S05U31006707 10, 21 -- for {microscopy-at-microscopy.com} ; Thu, 27 Oct 2005 17:05:33 -0700 (PDT) 10, 21 -- X-Sender: jmkrupp-at-cruzmail.ucsc.edu 10, 21 -- Message-Id: {v01550102bf871a0c3c57-at-[128.114.25.209]} 10, 21 -- Mime-Version: 1.0 10, 21 -- Content-Type: text/plain; charset="us-ascii" 10, 21 -- Date: Thu, 27 Oct 2005 17:01:42 -0700 10, 21 -- To: microscopy-at-microscopy.com 10, 21 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp) 10, 21 -- Subject: LD vs 'regular' LM objectives 10, 21 -- X-UCSC-CATS-Information: This message was scanned by the ITS MailScanner 10, 21 -- X-UCSC-CATS-MailScanner: Found to be clean 10, 21 -- X-UCSC-CATS-MailScanner-SpamCheck: not spam, spamassassin (score=1.57, 10, 21 -- required 8, autolearn=disabled, MISSING_SUBJECT 1.57) 10, 21 -- X-UCSC-CATS-MailScanner-SpamScore: s 10, 21 -- X-UCSC-CATS-MailScanner-From: jmkrupp-at-cats.ucsc.edu ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (payton-at-auburn.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, October 27, 2005 at 09:13:55 ---------------------------------------------------------------------------
Email: payton-at-auburn.edu Name: Lewis Payton
Organization: Auburn University
Title-Subject: [Filtered] MListserver: Coating Ciruit Board cross-sections for SEM analysis
Question: Hello,
Can anyone recommend a system for carbon coating or gold coating an SEM specimen.
We are cross-sectioning circuit boards to analyze the solder balls. Unfortunately, the circuit board edging is non-conductive and scattering the beam.
We've used a gold coater and a carbon coater with good success, but buying one is prohibitively expensive it seems (as is renting the one we are using per sample).
I'd greatly appreciate any advice anyone might have in general.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (avallecorsa-at-ups.edu) from http://www.microscopy.com/MLFormMail.html on Thursday, October 27, 2005 at 11:21:06 ---------------------------------------------------------------------------
Email: avallecorsa-at-ups.edu Name: Al vallecorsa
Organization: University of Puget Sound
Title-Subject: [Filtered] MListserver:
Question: We have an old Emscope SP2000, that we would like to get working. Can anyone supply a manual or information about it. One of our professors bought it used on E-bay. Thank You, Al
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dhorne-at-interchange.ubc.ca) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, October 27, 2005 at 14:20:48 ---------------------------------------------------------------------------
Title-Subject: [Filtered] MListserver: Platelet isolation for SEM
Question: Anyone have experience isolating platelets (non-adherent) without activating them for SEM imaging?
I need a method for a 2nd year student and I'm not sure in what direction to take him. He has already tried fixing the plasma, with the expected gelatinous goopy result. How about diluting the PRP (platelet-rich plasma) with PB/saline and filtering it prior to fixation?
I've come across a size-exclusion chromatography method, but we are not setup to do that in our lab.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mpease-at-jhmi.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Thursday, October 27, 2005 at 15:07:40 ---------------------------------------------------------------------------
Email: mpease-at-jhmi.edu Name: Mary Ellen Pease
Organization: Johns Hopkins University School of Medicine
Title-Subject: [Filtered] MListserver:
Question: I am interested in learning the osmolarity of PBS as compared to 0.1M Sorenson's Phosphate buffer. I can find osmolarity info on Sorenson's but not on PBS. A colleague of mine perserved tissues intended for epoxy processing in fixative made in PBS rather than Sorenson's. Any idea if this will compromise the morphology in 1um, tol blue stained sections?
osmolarity of hydrous solutions, especially of "buffers" - as I know the item - always depends on concentration of } effective { ions in solution. So, in the case of 0.1 M Soerensen buffer you will have in solution a certain amount of ions (and, because it is a PO4 buffer, cf. dissociation of substances/ions, you will have some material which is not dissolved/in solution).... - as you said - you will have some measure(s) on the osmolarity/osmolality of (assuming 0.15M) Soerensen phosphate buffer--} how much is your measure for this solution?.
If you have "PBS", you have to know the concentration (in M) or have to calculate ionic pressure by the amount of buffer salt(s) in the solution.
Also not to forget that higher concentrated PO4-buffer solutions (being it either NaOH-NaH2PO4 or Na2HPO4-NaH2PO4-mixtures) you hardly will be able to measure correctly, e.g. by an osmometer....this is due to the uncomplete dissociation of the PO4 ions if the ionic strength is high (e.g.0.2M: here you will not get an exact AND reproducible mosmol-value). If you dilute your buffer, say to 0.1M, or 0.05M you will see that measurement by an osmometer will result in a (more) stable and therefore correct value.
As a classical "measure" you will find } Millonig's { 0.13 M NaOH-Na2HPO4 -buffer at around 290-295 mosmol, and if you are adding some Sucrose (Saccharose) or better Glucose, you will get a 0.13 M NaPO4 buffer (pH around 7.2-7.4) amounting 300 mosmol (isotonicity of human blood).
If you need further information on that, please specify the concentration of your PBS (or ingredients), perhaps one can calculate the osmolarity
best regards
Wolfgang Muss
Personal Communication Data: OR Dr. Wolfgang Muss Member of EM-Lab Institute of Pathology, SALK (Salzburger Landeskliniken gemeinnuetzige GesmbH) Muellner Hauptstrasse 48 A-5020 SALZBURG AUSTRIA/EUROPE
and/or/alternatively
Paracelsus Medical Private University (PMU) Institute of Pathology Electron Microscopy Lab Muellner Hauptstrasse 48 A-5020 SALZBURG, Austria/Europe Phone work: +43+662+4482+4720 Mobile phone work:+43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss") E-Mail work: W.Muss-at-SALK.at
---------- Von: mpease-at-jhmi.edu[SMTP:mpease-at-jhmi.edu] Antwort an: mpease-at-jhmi.edu Gesendet: Freitag, 28. Oktober 2005 02:39 An: W.Muss-at-salk.at Betreff: [Microscopy] osmolarity of PBS
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mpease-at-jhmi.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Thursday, October 27, 2005 at 15:07:40 ---------------------------------------------------------------------------
Email: mpease-at-jhmi.edu Name: Mary Ellen Pease
Organization: Johns Hopkins University School of Medicine
Title-Subject: [Filtered] MListserver:
Question: I am interested in learning the osmolarity of PBS as compared to 0.1M Sorenson's Phosphate buffer. I can find osmolarity info on Sorenson's but not on PBS. A colleague of mine perserved tissues intended for epoxy processing in fixative made in PBS rather than Sorenson's. Any idea if this will compromise the morphology in 1um, tol blue stained sections?
We - Quorum Technologies - can mail you a copy of the original SP2000 operating manual, including wiring diagrams. Please be aware that the SP2000 was discontinued around 15 years ago and so availability or spares is likely to be limited. You may like to contact our local (US) distributor Energy Beam Sciences (http://www.ebsciences.com/) for further information and assistance.
Our relationship to "Emscope" products is now somewhat tenuous, but back in 1988 the UK based Emscope company was acquired by Bio-rad, the then owners of the Polaron range - now manufactured by Quorum Technologies (for a potted history see: http://www.quorumtech.com/history.htm)
Incidentally, many operating manuals for old products are available as downloads from our website - including the Emscope SC500 / SC500A sputter coater (see: http://www.quorumtech.com/Tech_Support/old-manuals.htm)
Best regards,
Mike Wombwell Sales & Marketing Director Quorum Technologies Newhaven, East Sussex, UK Tel: +44(0)1273 510535 Tel: +44(0)1273 511063 (direct) Fax: +44(0)1273 510536 mike.wombwell-at-quorumtech.com http://www.quorumtech.com E & O E
-----Original Message----- X-from: avallecorsa-at-ups.edu [mailto:avallecorsa-at-ups.edu] Sent: 28 October 2005 01:46 To: Mike Wombwell
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (avallecorsa-at-ups.edu) from http://www.microscopy.com/MLFormMail.html on Thursday, October 27, 2005 at 11:21:06 ------------------------------------------------------------------------ ---
Email: avallecorsa-at-ups.edu Name: Al vallecorsa
Organization: University of Puget Sound
Title-Subject: [Filtered] MListserver:
Question: We have an old Emscope SP2000, that we would like to get working. Can anyone supply a manual or information about it. One of our professors bought it used on E-bay. Thank You, Al
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (paults3gj-at-yahoo.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, October 28, 2005 at 00:58:05 ---------------------------------------------------------------------------
Email: paults3gj-at-yahoo.com Name: Paul S
Organization: CWRU
Title-Subject: [Filtered] MListserver: ISI TV mini-SEM documentation needed
Question: I have an ISI TV mini-SEM model M-RS-2-2 made in 1975. It appears to have all the required parts. I'm trying to get this unit operating and need service documentation. I'd be happy to just get a copy of the complete electrical schematic.
If anyone knows where to get documentation or spare parts for this SEM or if anyone could provide me with an eletrical schematic, please let me know.
If anyone has used this SEM and could give me their general impression of its performance (mag, resolution, etc), I'd also appreciate it.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ludovic.pinier-at-thalesgroup.com) from http://www.microscopy.com/MLFormMail.html on Friday, October 28, 2005 at 03:21:50 ---------------------------------------------------------------------------
Title-Subject: [Filtered] looking for TOPCON ABT-150F parts
Question: Hello microscopists,
I work on a TOPCON ABT-150F microscope. It seems to be a quite rare SEM, that is no more supported by the manufacturer. I need gaskets because of filament replacement and I don't know if they still are commercially available.
Does anyone knowing that microscope can tell me where to find replacement parts ?
The quality of the image and its ability to capture light (i.e., brightness of fluorescence images) is determined by the numerical aperture (NA) and the magnification of the objective. Long working distance objectives generally have a lower NA so they produce lower resolution and less bright fluorescent images than standard working distance objectives with similar magnifications but higher NA's. For a given magnification, the higher the NA, the brighter and sharper the image. For a given NA, the lower the magnification, the brighter the image but I think you need to know the actual NA and mag to predict the effect on resolution.
At 07:07 PM 10/27/05, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
I have heard from my electronic colleague that there are heating-tape, heating-cable, heating-pad, and heating-block you can use. Heating-tape of different lengths may be purchased at Canadian Tire. The length you need depends upon the dimensions, temperature requirement, and heat loss (insulated?). I believe there is an electronic shop, in you hospital, where you can seek help. Otherwise, write to me off list, I will ask my colleague to do some calculation and advise what the best choice is.
Ann Fook Yang EM Unit/ Unite EM AAFC/AAC 960 Carling Ave, Ottawa,Ontario Canada K1A 0C6 yanga-at-agr.gc.ca Telephone/Téléphone: 613-759-1638 Facsimile/Télécopieur: 613-759-1701
Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada
-----Original Message----- X-from: TrogadisJ-at-smh.toronto.on.ca [mailto:TrogadisJ-at-smh.toronto.on.ca] Sent: Thursday, October 27, 2005 5:12 PM To: Yang, Ann-Fook
Fellow Microscopists
We are looking for a device for maintaining a constant temperature inside a plexiglass incubator which is installed around the stage of an inverted microscope. Volume around 2 cu. ft.
We now have an external heating unit with temperature control that blows warm air into the incubator, however the air then flows out through small openings and it is difficult to regulate the CO2 levels because the air is constantly changing. Besides, the warm air results in fluid evaporation.
Ideally, I'm looking for a radiant source of heat to place inside the incubator - no light bulbs, for obvious reasons. We do have a thermistor to regulate the temperature into which the device could be plugged.
Any suggestions? Thank you Judy
Judy Trogadis Bio-Imaging Coordinator St. Michael's Hospital, 7Queen 30 Bond St. Toronto, ON M5B 1W8 Canada ph: 416-864-6060 x6337 pager: 416-685-9219 fax: 416-864-6043 trogadisj-at-smh.toronto.on.ca
==============================Original Headers============================== 7, 19 -- From trogadisj-at-smh.toronto.on.ca Thu Oct 27 16:09:45 2005 7, 19 -- Received: from smh.toronto.on.ca (mail.smh.toronto.on.ca [199.71.175.103]) 7, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9RL9jt7027278 7, 19 -- for {microscopy-at-msa.microscopy.com} ; Thu, 27 Oct 2005 16:09:45 -0500 7, 19 -- Received: from ([199.71.171.15]) 7, 19 -- by beethoven.smh.toronto.on.ca with ESMTP id KP-GCT47.5438033; 7, 19 -- Thu, 27 Oct 2005 17:09:20 -0400 7, 19 -- Received: from SMTPOUT-MTA by beethoven.smh.toronto.on.ca 7, 19 -- with Novell_GroupWise; Thu, 27 Oct 2005 17:09:20 -0400 7, 19 -- Message-Id: {s36109bf.006-at-beethoven.smh.toronto.on.ca} 7, 19 -- X-Mailer: Novell GroupWise Internet Agent 6.5.2 7, 19 -- Date: Thu, 27 Oct 2005 17:08:54 -0400 7, 19 -- From: "Judy Trogadis" {TrogadisJ-at-smh.toronto.on.ca} 7, 19 -- To: {Microscopy-at-msa.microscopy.com} 7, 19 -- Subject: heating live cells 7, 19 -- Mime-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=US-ASCII 7, 19 -- Content-Transfer-Encoding: 7bit 7, 19 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 15, 29 -- From YANGA-at-AGR.GC.CA Fri Oct 28 08:34:01 2005 15, 29 -- Received: from agroutsmtp1.agr.gc.ca (agroutsmtp1.agr.gc.ca [192.197.71.175]) 15, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9SDY1On021238 15, 29 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 08:34:01 -0500 15, 29 -- Received: from agrin1-old.agr.gc.ca (agrgate.agr.ca [192.197.71.189]) 15, 29 -- by agroutsmtp1.agr.gc.ca (8.12.8/8.12.8) with ESMTP id j9SDY1CD001751 15, 29 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 09:34:01 -0400 15, 29 -- Received: from onncrxcn1.AGR.GC.CA ([10.117.15.130]) 15, 29 -- by agrin1-old.agr.gc.ca (8.12.8/8.12.8) with ESMTP id j9SDYqgg018549 15, 29 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 09:34:52 -0400 15, 29 -- Received: from onncrxms3.AGR.GC.CA ([10.117.15.30]) by onncrxcn1.AGR.GC.CA with Microsoft SMTPSVC(5.0.2195.6713); 15, 29 -- Fri, 28 Oct 2005 09:33:55 -0400 15, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 15, 29 -- content-class: urn:content-classes:message 15, 29 -- MIME-Version: 1.0 15, 29 -- Content-Type: text/plain; 15, 29 -- charset="iso-8859-1" 15, 29 -- Subject: RE: [Microscopy] heating live cells 15, 29 -- Date: Fri, 28 Oct 2005 09:33:54 -0400 15, 29 -- Message-ID: {E035A9C87303AE4AB9BA10FD8324DFB1355449-at-onncrxms3.agr.gc.ca} 15, 29 -- X-MS-Has-Attach: 15, 29 -- X-MS-TNEF-Correlator: 15, 29 -- Thread-Topic: [Microscopy] heating live cells 15, 29 -- Thread-Index: AcXbOwVHRTJVO4B/QW2d6ZhH6om7pAAg86dw 15, 29 -- From: "Yang, Ann-Fook" {YANGA-at-AGR.GC.CA} 15, 29 -- To: {microscopy-at-microscopy.com} 15, 29 -- X-OriginalArrivalTime: 28 Oct 2005 13:33:55.0851 (UTC) FILETIME=[3E7EFDB0:01C5DBC4] 15, 29 -- Content-Transfer-Encoding: 8bit 15, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9SDY1On021238 ==============================End of - Headers==============================
} From mail-at-ns.microscopy.com Fri Oct 28 09:37:17 2005 } Return-Path: {mail-at-ns.microscopy.com} } Received: from ns.microscopy.com (microscopy.com [206.69.208.10]) } by www.matscieng.sunysb.edu (8.11.6/8.11.6) with ESMTP id j9SDbHT01707 } for {jquinn-at-www.matscieng.sunysb.edu} ; Fri, 28 Oct 2005 09:37:17 -0400 } Received: from ns.microscopy.com (localhost.localdomain [127.0.0.1]) } by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9SDYZj2022621 } for {jquinn-at-www.matscieng.sunysb.edu} ; Fri, 28 Oct 2005 08:34:35 -0500 } Received: (from mail-at-localhost) } by ns.microscopy.com (8.12.11/8.12.10/Submit) id j9SDYZ2c022619; } Fri, 28 Oct 2005 08:34:35 -0500 } Date: Fri, 28 Oct 2005 08:34:35 -0500 } Message-Id: {200510281334.j9SDYZ2c022619-at-ns.microscopy.com} } To: jquinn-at-www.matscieng.sunysb.edu } From: YANGA-at-AGR.GC.CA } Reply-to: YANGA-at-AGR.GC.CA } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] RE: heating live cells } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } Status: R } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Judy, } } I have heard from my electronic colleague that there are heating-tape, heating-cable, heating-pad, and heating-block you can use. Heating-tape of different lengths may be purchased at Canadian Tire. The length you need depends upon the dimensions, temperature requirement, and heat loss (insulated?). I believe there is an electronic shop, in you hospital, where you can seek help. Otherwise, write to me off list, I will ask my colleague to do some calculation and advise what the best choice is. } } Ann Fook Yang } EM Unit/ Unite EM } AAFC/AAC } 960 Carling Ave, } Ottawa,Ontario } Canada K1A 0C6 } yanga-at-agr.gc.ca } Telephone/Téléphone: 613-759-1638 } Facsimile/Télécopieur: 613-759-1701 } } Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada } } -----Original Message----- } X-from: TrogadisJ-at-smh.toronto.on.ca [mailto:TrogadisJ-at-smh.toronto.on.ca] } Sent: Thursday, October 27, 2005 5:12 PM } To: Yang, Ann-Fook } Subject: [Microscopy] heating live cells } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Fellow Microscopists } } We are looking for a device for maintaining a constant temperature } inside a plexiglass incubator which is installed around the stage of an } inverted microscope. Volume around 2 cu. ft. } } We now have an external heating unit with temperature control that } blows warm air into the incubator, however the air then flows out } through small openings and it is difficult to regulate the CO2 levels } because the air is constantly changing. Besides, the warm air results in } fluid evaporation. } } Ideally, I'm looking for a radiant source of heat to place inside the } incubator - no light bulbs, for obvious reasons. We do have a thermistor } to regulate the temperature into which the device could be plugged. } } Any suggestions? } Thank you } Judy } } Judy Trogadis } Bio-Imaging Coordinator } St. Michael's Hospital, 7Queen } 30 Bond St. } Toronto, ON M5B 1W8 } Canada } ph: 416-864-6060 x6337 } pager: 416-685-9219 } fax: 416-864-6043 } trogadisj-at-smh.toronto.on.ca } } } ==============================Original Headers============================== } 7, 19 -- From trogadisj-at-smh.toronto.on.ca Thu Oct 27 16:09:45 2005 } 7, 19 -- Received: from smh.toronto.on.ca (mail.smh.toronto.on.ca [199.71.175.103]) } 7, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9RL9jt7027278 } 7, 19 -- for {microscopy-at-msa.microscopy.com} ; Thu, 27 Oct 2005 16:09:45 -0500 } 7, 19 -- Received: from ([199.71.171.15]) } 7, 19 -- by beethoven.smh.toronto.on.ca with ESMTP id KP-GCT47.5438033; } 7, 19 -- Thu, 27 Oct 2005 17:09:20 -0400 } 7, 19 -- Received: from SMTPOUT-MTA by beethoven.smh.toronto.on.ca } 7, 19 -- with Novell_GroupWise; Thu, 27 Oct 2005 17:09:20 -0400 } 7, 19 -- Message-Id: {s36109bf.006-at-beethoven.smh.toronto.on.ca} } 7, 19 -- X-Mailer: Novell GroupWise Internet Agent 6.5.2 } 7, 19 -- Date: Thu, 27 Oct 2005 17:08:54 -0400 } 7, 19 -- From: "Judy Trogadis" {TrogadisJ-at-smh.toronto.on.ca} } 7, 19 -- To: {Microscopy-at-msa.microscopy.com} } 7, 19 -- Subject: heating live cells } 7, 19 -- Mime-Version: 1.0 } 7, 19 -- Content-Type: text/plain; charset=US-ASCII } 7, 19 -- Content-Transfer-Encoding: 7bit } 7, 19 -- Content-Disposition: inline } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 15, 29 -- From YANGA-at-AGR.GC.CA Fri Oct 28 08:34:01 2005 } 15, 29 -- Received: from agroutsmtp1.agr.gc.ca (agroutsmtp1.agr.gc.ca [192.197.71.175]) } 15, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9SDY1On021238 } 15, 29 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 08:34:01 -0500 } 15, 29 -- Received: from agrin1-old.agr.gc.ca (agrgate.agr.ca [192.197.71.189]) } 15, 29 -- by agroutsmtp1.agr.gc.ca (8.12.8/8.12.8) with ESMTP id j9SDY1CD001751 } 15, 29 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 09:34:01 -0400 } 15, 29 -- Received: from onncrxcn1.AGR.GC.CA ([10.117.15.130]) } 15, 29 -- by agrin1-old.agr.gc.ca (8.12.8/8.12.8) with ESMTP id j9SDYqgg018549 } 15, 29 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 09:34:52 -0400 } 15, 29 -- Received: from onncrxms3.AGR.GC.CA ([10.117.15.30]) by onncrxcn1.AGR.GC.CA with Microsoft SMTPSVC(5.0.2195.6713); } 15, 29 -- Fri, 28 Oct 2005 09:33:55 -0400 } 15, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 } 15, 29 -- content-class: urn:content-classes:message } 15, 29 -- MIME-Version: 1.0 } 15, 29 -- Content-Type: text/plain; } 15, 29 -- charset="iso-8859-1" } 15, 29 -- Subject: RE: [Microscopy] heating live cells } 15, 29 -- Date: Fri, 28 Oct 2005 09:33:54 -0400 } 15, 29 -- Message-ID: {E035A9C87303AE4AB9BA10FD8324DFB1355449-at-onncrxms3.agr.gc.ca} } 15, 29 -- X-MS-Has-Attach: } 15, 29 -- X-MS-TNEF-Correlator: } 15, 29 -- Thread-Topic: [Microscopy] heating live cells } 15, 29 -- Thread-Index: AcXbOwVHRTJVO4B/QW2d6ZhH6om7pAAg86dw } 15, 29 -- From: "Yang, Ann-Fook" {YANGA-at-AGR.GC.CA} } 15, 29 -- To: {microscopy-at-microscopy.com} } 15, 29 -- X-OriginalArrivalTime: 28 Oct 2005 13:33:55.0851 (UTC) FILETIME=[3E7EFDB0:01C5DBC4] } 15, 29 -- Content-Transfer-Encoding: 8bit } 15, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9SDY1On021238 } ==============================End of - Headers============================== }
==============================Original Headers============================== 10, 12 -- From jquinn-at-www.matscieng.sunysb.edu Fri Oct 28 08:43:20 2005 10, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 10, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9SDhI5q029920 10, 12 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 28 Oct 2005 08:43:19 -0500 10, 12 -- Received: (from jquinn-at-localhost) 10, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id j9SDjsP02017 10, 12 -- for Microscopy-at-msa.microscopy.com; Fri, 28 Oct 2005 09:45:54 -0400 10, 12 -- Date: Fri, 28 Oct 2005 09:45:54 -0400 10, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 10, 12 -- Message-Id: {200510281345.j9SDjsP02017-at-www.matscieng.sunysb.edu} 10, 12 -- To: Microscopy-at-msa.microscopy.com 10, 12 -- Subject: re: Heating live cells ==============================End of - Headers==============================
There's also an inexpensive package that Thierry Epicier built, based on the SHRLI code I wrote waaay back ("Computed crystal structure images for high resolution electron microscopy", M.A. O'Keefe, P.R. Buseck and S. Iijima, Nature 274 (1978) 322-324).
A freeware version of Thierry's image and diffraction package can be found at: http://www.amc.anl.gov/ANLSoftwareLibrary/02-MMSLib/HREM/shrli/readme.txt
Alternatively, see the Listserver archives -- go to http://www.microscopy.com/cgi-bin/ReadPrintEmailHTML.pl?filename=9411.txt and do a find (ctrl-F) for SHRLI.
I haven't worked in this field for quite a while and I won't have access to a working copy for the foreseeable future.
Mike ----- Original Message ----- X-from: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu}
I don't want to start a flame war, I don't wish to lose my access to this list server......
Your statement is only part of the story. Image quality is also affected by the degree of correction built into the objective. An achromatic objective may have corrections for one color spherical aberration and two colors chromatic aberration. An apochromatic would have corrections for two color spherical aberration and three colors chromatic aberration. I will not even attempt to fit in flat fields, fluorites, semifluorites and the old fashion gem lens. With that we still have a series of corrections for comma, flare, barrel and goodness knows what else.
So, Given two objectives of the same quality and correction, I would agree with you, bigger NA means more potential resolving power up to the NA of the substage condenser as limited by the refractive index of air. (Now we're into oil immersion systems!) Remember we're are talking about an optical system of condenser and objective.
I tell everyone about examining diatoms with two different objectives, one from company N, the other from company Z (maybe 30 years ago). The two objectives were the same magnification and had roughly the same NA, but quality was significantly different. The Z objective made diatom structure features jump out at me. With the N objective I could find the features but I had to hunt for them.
There is more to examining images than resolving power.....
Frank Karl Degussa Corporation Akron Technical Center 3500 Embassy Parkway Suite 100 Akron, Ohio 44333
330-668-2235 Ext. 238
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phillipst-at-missour i.edu To: frank.karl-at-degussa.com cc: 10/28/2005 09:23 Subject: [Microscopy] Re: LD vs 'regular' LM objectives AM Please respond to phillipst
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
The quality of the image and its ability to capture light (i.e., brightness
of fluorescence images) is determined by the numerical aperture (NA) and the magnification of the objective. Long working distance objectives generally have a lower NA so they produce lower resolution and less bright fluorescent images than standard working distance objectives with similar magnifications but higher NA's. For a given magnification, the higher the NA, the brighter and sharper the image. For a given NA, the lower the magnification, the brighter the image but I think you need to know the actual NA and mag to predict the effect on resolution.
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} } Hi } } Anyone like to offer an opinion of the differences in image quality between } LD and standard objective lenses for light microscopy including } fluorescence? } } We have a 'hybrid' scope, some LD, some standard objectives and are trying } to decide which kind to add to go to make them all the same. } } Pretty general question, I know, but give it a shot if you have some ideas. } } Thanks } } Jon } } } Jonathan Krupp } Microscopy & Imaging Lab } C230 Earth & Marine Science } University of California } Santa Cruz, CA 95064 } (831) 459-2477 } jmkrupp-at-cats.ucsc.edu } } } } ==============================Original Headers============================== } 10, 21 -- From jmkrupp-at-cats.ucsc.edu Thu Oct 27 19:06:28 2005 } 10, 21 -- Received: from cats-mx1.ucsc.edu (cats-mx1.ucsc.edu } [128.114.125.34]) } 10, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j9S06RLq022715 } 10, 21 -- for {microscopy-at-microscopy.com} ; Thu, 27 Oct 2005 19:06:28
} -0500 } 10, 21 -- Received: from [128.114.25.209] (dhcp-25-209.ucsc.edu } [128.114.25.209]) } 10, 21 -- by cats-mx1.ucsc.edu (8.13.1/8.13.1) with SMTP id } j9S05U31006707 } 10, 21 -- for {microscopy-at-microscopy.com} ; Thu, 27 Oct 2005 17:05:33
} -0700 (PDT) } 10, 21 -- X-Sender: jmkrupp-at-cruzmail.ucsc.edu } 10, 21 -- Message-Id: {v01550102bf871a0c3c57-at-[128.114.25.209]} } 10, 21 -- Mime-Version: 1.0 } 10, 21 -- Content-Type: text/plain; charset="us-ascii" } 10, 21 -- Date: Thu, 27 Oct 2005 17:01:42 -0700 } 10, 21 -- To: microscopy-at-microscopy.com } 10, 21 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp) } 10, 21 -- Subject: LD vs 'regular' LM objectives } 10, 21 -- X-UCSC-CATS-Information: This message was scanned by the ITS } MailScanner } 10, 21 -- X-UCSC-CATS-MailScanner: Found to be clean } 10, 21 -- X-UCSC-CATS-MailScanner-SpamCheck: not spam, spamassassin } (score=1.57, } 10, 21 -- required 8, autolearn=disabled, MISSING_SUBJECT 1.57) } 10, 21 -- X-UCSC-CATS-MailScanner-SpamScore: s } 10, 21 -- X-UCSC-CATS-MailScanner-From: jmkrupp-at-cats.ucsc.edu } ==============================End of - Headers==============================
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
We only have one Zeiss 'air' LD plan neofluar - a 63x. However it's image quality is noticeably poorer than our Zeiss oil immersion 63x Plan Apochromat's, using confocal and standard fluorescence imaging configurations. The LD objective was over £1,000 cheaper - we use it for both epi-fluorescence and Ph transmission.
Our LD obviously scores if you want to image something 1mm into the sample though owing to its very long working distance (WD 1.2 to 2.2 mm). In comparison our Plan Apochromat high power oil objectives (WD = 0.19 mm) only just get into the cells stuck onto the coverslip (e.g. using Mattek dishes) and we can't see anything at all with a slightly raised coverslip on slides.
On our Leica SP2 AOBS confocal system, our 'blue' Leica 20x HCL PL APO water/glycerol/oil objective has very good image quality and a slightly bigger WD with water compared to oil immersion (free WD = 0.26 mm water or 0.17 mm oil). However water immersion is a pain with the inverted microscopes we use.
Our local Zeiss rep is always happy to lend us objectives for appraisal, which is extremely useful.
Regards
Keith
PS. Years ago at a meeting I heard that higher NA increases the resolution, but lowering the NA (assuming you have a variable NA collar on your objective) improves the contrast, which might be more useful in some cases. It seems to work with the one variable NA objective we have.
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
----- Original Message ----- X-from: {jmkrupp-at-cats.ucsc.edu} To: {keith.morris-at-ucl.ac.uk} Sent: Friday, October 28, 2005 1:11 AM
Hi Judy,
I use fixed temperature 'convection enclosure heaters' (see RSWWW link). They are really designed for eliminating condensation in outside enclosures, but they simply plug in and then go to a set surface temperature with no thermostat control. They are designed to stay on permanently and have overheat & thermal fuse protection etc..
I have two 30w enclosure heaters to supply additional heat inside a large Perspex microscope incubator (similar to www.solent-scientific.co.uk ones). They are fitted into little aluminium stands our workshop made for them. With the thermostatically controlled 'air blower' off & the enclosure heaters on, the incubator stays around 32oC ready to be quickly heated when the Zeiss/PeCon CTI/3700 'air blow temp' controllers are switched on. I do put bubble wrap on the back, sides and top of the incubator, to reduce heat loss to the room.
See them at http://www.rswww.com and search for item 224-492 .
They may suit. They are cheap and reasonably compact.
The 30W ones get to around 57oC surface temp when left on, and should be placed a few cm's from cables etc... The stands we built for them also shield the side surfaces from fingers as otherwise they are hot to touch (the higher the wattage the hotter they get).
Regards
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
----- Original Message ----- } From: {TrogadisJ-at-smh.toronto.on.ca} } To: {keith.morris-at-ucl.ac.uk} } Sent: Thursday, October 27, 2005 10:16 PM } Subject: [Microscopy] heating live cells } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Fellow Microscopists } } } } We are looking for a device for maintaining a constant temperature } } inside a plexiglass incubator which is installed around the stage of an } } inverted microscope. Volume around 2 cu. ft. } } } } We now have an external heating unit with temperature control that } } blows warm air into the incubator, however the air then flows out } } through small openings and it is difficult to regulate the CO2 levels } } because the air is constantly changing. Besides, the warm air results in } } fluid evaporation. } } } } Ideally, I'm looking for a radiant source of heat to place inside the } } incubator - no light bulbs, for obvious reasons. We do have a thermistor } } to regulate the temperature into which the device could be plugged. } } } } Any suggestions? } } Thank you } } Judy } } } } Judy Trogadis } } Bio-Imaging Coordinator } } St. Michael's Hospital, 7Queen } } 30 Bond St. } } Toronto, ON M5B 1W8 } } Canada } } ph: 416-864-6060 x6337 } } pager: 416-685-9219 } } fax: 416-864-6043 } } trogadisj-at-smh.toronto.on.ca } } } } } } ==============================Original } } Headers============================== } } 7, 19 -- From trogadisj-at-smh.toronto.on.ca Thu Oct 27 16:09:45 2005 } } 7, 19 -- Received: from smh.toronto.on.ca (mail.smh.toronto.on.ca } } [199.71.175.103]) } } 7, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } } j9RL9jt7027278 } } 7, 19 -- for {microscopy-at-msa.microscopy.com} ; Thu, 27 Oct 2005 } } 16:09:45 -0500 } } 7, 19 -- Received: from ([199.71.171.15]) } } 7, 19 -- by beethoven.smh.toronto.on.ca with ESMTP id KP-GCT47.5438033; } } 7, 19 -- Thu, 27 Oct 2005 17:09:20 -0400 } } 7, 19 -- Received: from SMTPOUT-MTA by beethoven.smh.toronto.on.ca } } 7, 19 -- with Novell_GroupWise; Thu, 27 Oct 2005 17:09:20 -0400 } } 7, 19 -- Message-Id: {s36109bf.006-at-beethoven.smh.toronto.on.ca} } } 7, 19 -- X-Mailer: Novell GroupWise Internet Agent 6.5.2 } } 7, 19 -- Date: Thu, 27 Oct 2005 17:08:54 -0400 } } 7, 19 -- From: "Judy Trogadis" {TrogadisJ-at-smh.toronto.on.ca} } } 7, 19 -- To: {Microscopy-at-msa.microscopy.com} } } 7, 19 -- Subject: heating live cells } } 7, 19 -- Mime-Version: 1.0 } } 7, 19 -- Content-Type: text/plain; charset=US-ASCII } } 7, 19 -- Content-Transfer-Encoding: 7bit } } 7, 19 -- Content-Disposition: inline } } ==============================End of - } } Headers============================== } } } } }
==============================Original Headers============================== 13, 27 -- From keith.morris-at-ucl.ac.uk Fri Oct 28 10:39:05 2005 13, 27 -- Received: from vscani-e2.ucl.ac.uk (vscani-e2.ucl.ac.uk [144.82.108.34]) 13, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9SFd4kO001070 13, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 10:39:04 -0500 13, 27 -- Received: from cell23.ioo.ucl.ac.uk ([144.82.134.123] helo=keithhigrade) 13, 27 -- by vscani-e.ucl.ac.uk with smtp (Exim 4.51) 13, 27 -- id 1EVWJo-0000yf-9I 13, 27 -- for Microscopy-at-microscopy.com; Fri, 28 Oct 2005 16:39:00 +0100 13, 27 -- Message-ID: {006e01c5dbd5$a6331610$7b865290-at-keithhigrade} 13, 27 -- From: "Keith J Morris" {keith.morris-at-ucl.ac.uk} 13, 27 -- To: {Microscopy-at-microscopy.com} 13, 27 -- Subject: Fw: [Microscopy] heating live cells 13, 27 -- Date: Fri, 28 Oct 2005 16:38:31 +0100 13, 27 -- MIME-Version: 1.0 13, 27 -- Content-Type: text/plain; 13, 27 -- format=flowed; 13, 27 -- charset="iso-8859-1"; 13, 27 -- reply-type=original 13, 27 -- Content-Transfer-Encoding: 7bit 13, 27 -- X-Priority: 3 13, 27 -- X-MSMail-Priority: Normal 13, 27 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2670 13, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2670 13, 27 -- X-UCL-MailScanner-Information: Please contact the UCL Helpdesk, helpdesk-at-ucl.ac.uk for more information 13, 27 -- X-UCL-MailScanner: Found to be clean 13, 27 -- X-UCL-MailScanner-SpamCheck: 13, 27 -- X-MailScanner-From: keith.morris-at-ucl.ac.uk ==============================End of - Headers==============================
Hello Randy, I am a grad student at NMSU and have been working some immunolabelling. We are looking at localization of GFP labelled bacteria in squid tissue. So far I have had luck immunolbelling GFP transformed bacteria with colloidal gold. I am using LR white for embedding. I haven't encountered any problems thus far with immunolabelling. The only problem I have is that I have a huge tissue and I need to section transversly thru the entire tissue. This leaves a lot of wrinkles behind after the whole process of immunlabeling which does not look real good after scanning the negatives. The sections are very thin to withstand stretching using chloroform and other resins seem to affect immunolabelling. This is where I could use some help. Would it be ethical to try and iron out the wrinkles using any software. I would greatly appreciate any suggestions and or solutions to iron out those wrinkles. Unfortunately re embedding is not an option as we do not have any more tissue. Desperate to graduate :) Vinod
==============================Original Headers============================== 2, 23 -- From nairvinods-at-gmail.com Fri Oct 28 12:33:28 2005 2, 23 -- Received: from xproxy.gmail.com (xproxy.gmail.com [66.249.82.207]) 2, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9SHXSUT011440 2, 23 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 12:33:28 -0500 2, 23 -- Received: by xproxy.gmail.com with SMTP id s13so420281wxc 2, 23 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 10:33:28 -0700 (PDT) 2, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 2, 23 -- s=beta; d=gmail.com; 2, 23 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 2, 23 -- b=eVDpSxLinOQAaa52VnTcQuABrNTPX5h9YIM9rHP9pl5hAudAHoVza8cVcNTBHxZ7Nyj81llJUJIGI3aR1uWnzl6qq+HtxzDoE12TDGoF+dv2jY84UMi99UMKjGvfKjXw4BbwzOAjmJMckm+ZPLn4fnZvzBvcMVC8SFO3WsLSbUE= 2, 23 -- Received: by 10.65.220.8 with SMTP id x8mr273279qbq; 2, 23 -- Fri, 28 Oct 2005 10:33:28 -0700 (PDT) 2, 23 -- Received: by 10.64.201.9 with HTTP; Fri, 28 Oct 2005 10:33:28 -0700 (PDT) 2, 23 -- Message-ID: {ea42a3900510281033x277c9781u9c787810447c8c0b-at-mail.gmail.com} 2, 23 -- Date: Fri, 28 Oct 2005 11:33:28 -0600 2, 23 -- From: Vinod Nair {nairvinods-at-gmail.com} 2, 23 -- To: microscopy-at-microscopy.com 2, 23 -- Subject: Immuno TEM ....GFP 2, 23 -- MIME-Version: 1.0 2, 23 -- Content-Type: text/plain; charset=UTF-8 2, 23 -- Content-Disposition: inline 2, 23 -- Content-Transfer-Encoding: 8bit 2, 23 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id j9SHXSUT011440 ==============================End of - Headers==============================
A graduate student in the lab routinely uses gold conjugated anti-GFP secondary antibody to label GFP in LR White embedded tissue. It is post section labeling and works just fine. You can buy the secondary ab from I believe Jackson Immunoresearch Lab.
Can you Fed Ex me some left overs from your dinner party?
Soumitra
Quoting TindallR-at-missouri.edu:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } I'm curious to see if anyone has any words of wisdom about } immunostaining of GFP in general. I'd be interested in experiences } relating to DAB-HRP labeling and/or immunogold, both pre- and } post-embedding. Cryosections, too! (There, that ought to keep you } busy.) } } More specifically, are there any danger spots in the fixation or } embedding processes that could negatively affect the labeling process, } or even destroy the protein itself? } } I know it's one of those overly broad questions, but I am mainly } interested in how, if at all, labeling GFP might be different that } labeling other proteins. I've not had much luck going through our } library on finding GFP-specific material, and I'm still Googling away. } } By the way, you're all invited to dinner at my place tonight by way of } thanks. My Honduran in-laws are in town and the food is good and } plentiful and the music is Latin. Yes, people CAN cook and dance at the } same time. } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W122 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } } } } } } } } ==============================Original } Headers============================== } 10, 23 -- From TindallR-at-missouri.edu Thu Oct 27 12:43:35 2005 } 10, 23 -- Received: from um-exproto9.um.umsystem.edu } (um-exproto9.um.umsystem.edu [207.160.151.49]) } 10, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } j9RHhZAB030929 } 10, 23 -- for {microscopy-at-microscopy.com} ; Thu, 27 Oct 2005 12:43:35 -0500 } 10, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by } um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); } 10, 23 -- Thu, 27 Oct 2005 12:43:34 -0500 } 10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 } 10, 23 -- Content-class: urn:content-classes:message } 10, 23 -- MIME-Version: 1.0 } 10, 23 -- Content-Type: text/plain; } 10, 23 -- charset="us-ascii" } 10, 23 -- Subject: Immuno EM---GFP } 10, 23 -- Date: Thu, 27 Oct 2005 12:43:34 -0500 } 10, 23 -- Message-ID: } {BA876152E8653240BE8572E897083EE79804BC-at-UM-EMAIL09.um.umsystem.edu} } 10, 23 -- X-MS-Has-Attach: } 10, 23 -- X-MS-TNEF-Correlator: } 10, 23 -- Thread-Topic: Immuno EM---GFP } 10, 23 -- Thread-Index: AcXbHfQFbshrz551RZu1z6r4WMdnPg== } 10, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 10, 23 -- To: {microscopy-at-microscopy.com} } 10, 23 -- X-OriginalArrivalTime: 27 Oct 2005 17:43:34.0876 (UTC) } FILETIME=[F44711C0:01C5DB1D] } 10, 23 -- Content-Transfer-Encoding: 8bit } 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id j9RHhZAB030929 } ==============================End of - } Headers============================== }
Soumitra Ghoshroy College Associate Professor, Biology Director, Electron Microscopy Lab New Mexico State University Las Cruces, NM 88003 Tel: 505-646-3268 (office) 505-646-3283 (lab) Fax: 505-646-3282 http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm http://emldata.nmsu.edu/eml
==============================Original Headers============================== 9, 20 -- From sghoshro-at-NMSU.Edu Fri Oct 28 12:44:04 2005 9, 20 -- Received: from ccserver3.nmsu.edu (ccserver3.nmsu.edu [128.123.34.98]) 9, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9SHi3ax020223 9, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 12:44:03 -0500 9, 20 -- Received: from ccserver3.nmsu.edu (ccserver3.nmsu.edu [127.0.0.1]) 9, 20 -- by localhost (Postfix) with SMTP id A5FEB3FC263 9, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 11:44:03 -0600 (MDT) 9, 20 -- Received: from verdi (verdi.nmsu.edu [128.123.34.188]) 9, 20 -- by ccserver3.nmsu.edu (Postfix) with ESMTP id 6D7F73FC21F 9, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 11:44:03 -0600 (MDT) 9, 20 -- Date: Fri, 28 Oct 2005 11:44:03 -0600 (MDT) 9, 20 -- From: sghoshro-at-NMSU.Edu 9, 20 -- X-X-Sender: sghoshro-at-verdi 9, 20 -- To: Microscopy-at-microscopy.com 9, 20 -- Subject: Re: [Microscopy] Immuno EM---GFP 9, 20 -- Message-ID: {Pine.GSO.4.61.0510281142580.26557-at-verdi} 9, 20 -- MIME-Version: 1.0 9, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 9, 20 -- X-PMX-Version: 5.0.3.165339, Antispam-Engine: 2.1.0.0, Antispam-Data: 2005.10.28.18 9, 20 -- X-PerlMx-Spam: Gauge=IIIIIII, Probability=7%, Report='IP_HTTP_ADDR 0, NO_REAL_NAME 0, __C230066_P5 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_CRUFT 0' ==============================End of - Headers==============================
I've been alerted that funding may be available for the installation of magnetic strip card readers to tracking instrument use in my facility. Apparently they are being used successfully for this purpose in a university micro-fabrication facility in our state. If they work, perhaps it would simplify the accounting involved in monthly invoicing. However, I have a couple of concerns about using them in our facility that I can't work out. Maybe you can help.
Do you install the reader on the instrument itself, or on the doorway of the lab. This is the standard way of installing readers at my university. There is a problem when they are used on a lab door when there are several instruments inside.
Can readers be interlocked into the instruments to prevent use until a card is swiped? Has anyone done that? How?
If I use a swipe style reader, we could probably get compliance when a user gets started. However, we'd have to get them to swipe again at the end of their use to capture the elapsed time (which is what we really want!). If they don't swipe again at the end of their session, I'll have to spend more time determining how much time they used.
Have any of you used a card reader like the ones on ATM machines that actually "keep" your card until the transaction is completed? A reader like this might solve the problem above.
There is a problem for automated instruments (like microprobes) where a sample run may extend into the early morning hours. Since no one will be there to swipe at the end, how could we accurately track use in those situations?
I appreciate any input you can offer. Thanks!
Owen
Owen P. Mills Director, Materials Characterization and Fabrication Facilities Electron Optics Engineer, Applied Chemical & Morphological Analysis Laboratory Materials Science & Engineering Michigan Technological University Rm 512 M&M Bldg. Houghton, MI 49931 PH 906-369-1875 FAX 906-487-2934 mailto:opmills-at-mtu.edu http://www.mm.mtu.edu/~opmills
Owen P. Mills Director, Materials Characterization and Fabrication Facilities Electron Optics Engineer, Applied Chemical & Morphological Analysis Laboratory Materials Science & Engineering Michigan Technological University Rm 512 M&M Bldg. Houghton, MI 49931 PH 906-369-1875 FAX 906-487-2934 mailto:opmills-at-mtu.edu http://www.mm.mtu.edu/~opmills
==============================Original Headers============================== 17, 31 -- From opmills-at-mtu.edu Fri Oct 28 14:16:22 2005 17, 31 -- Received: from mailoff.mtu.edu (mailoff.mtu.edu [141.219.70.111]) 17, 31 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9SJGMcn029940 17, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 14:16:22 -0500 17, 31 -- Received: from node5.edge.dcsint.mtu.edu (node5.mtu.edu [141.219.69.5]) 17, 31 -- by mailoff.mtu.edu (8.11.7p1+Sun/8.11.4) with ESMTP id j9SJGLR18010 17, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 15:16:21 -0400 (EDT) 17, 31 -- Received: from node34.edge.dcsint.mtu.edu (node34.mtu.edu [141.219.69.34]) 17, 31 -- by node5.edge.dcsint.mtu.edu (8.12.10/8.12.8) with ESMTP id j9SJGLhv032418 17, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 15:16:21 -0400 17, 31 -- Received: from mail.mtu.edu (campus4.mtu.edu [141.219.70.7]) 17, 31 -- by node34.edge.dcsint.mtu.edu (8.12.11/8.12.11) with ESMTP id j9SJGKrI002888 17, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 15:16:20 -0400 17, 31 -- (envelope-from opmills-at-mtu.edu) 17, 31 -- Received: from node6.edge.dcsint.mtu.edu (node6.mtu.edu [141.219.69.6]) 17, 31 -- by mail.mtu.edu (8.11.7p1+Sun/8.11.6) with ESMTP id j9SJGIt21560; 17, 31 -- Fri, 28 Oct 2005 15:16:18 -0400 (EDT) 17, 31 -- Received: from [141.219.192.45] (opmills.eecn.sabo.mtu.edu [141.219.192.45]) 17, 31 -- by node6.edge.dcsint.mtu.edu (8.12.10/8.12.3/auth_ssl.mc v1.0) with ESMTP id j9SJGIH0023657; 17, 31 -- Fri, 28 Oct 2005 15:16:18 -0400 17, 31 -- Mime-Version: 1.0 (Apple Message framework v734) 17, 31 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 17, 31 -- Message-Id: {9CE4B27D-F4C4-4B72-A28C-5628CE214BE6-at-mtu.edu} 17, 31 -- Content-Transfer-Encoding: 7bit 17, 31 -- From: "Owen P. Mills" {opmills-at-mtu.edu} 17, 31 -- Subject: [Microscopy] Magnetic card readers for instrument access 17, 31 -- Date: Fri, 28 Oct 2005 15:19:58 -0400 17, 31 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 17, 31 -- X-Mailer: Apple Mail (2.734) 17, 31 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.0.3.2, Antispam-Data: 2005.10.28.22 17, 31 -- X-PerlMx-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
} Fellow Microscopists } } We are looking for a device for maintaining a constant temperature } inside a plexiglass incubator which is installed around the stage of an } inverted microscope. Volume around 2 cu. ft. } } We now have an external heating unit with temperature control that } blows warm air into the incubator, however the air then flows out } through small openings and it is difficult to regulate the CO2 levels } because the air is constantly changing. Besides, the warm air results in } fluid evaporation. } } Ideally, I'm looking for a radiant source of heat to place inside the } incubator - no light bulbs, for obvious reasons. We do have a thermistor } to regulate the temperature into which the device could be plugged. } } Any suggestions? } Thank you } Judy } } Judy Trogadis
Hi Judy,
If you need an out of the box solution a look for PID temperature control such as http://www.watlow.com/products/controllers/ and for that small chamber I would use several Caddock resistors http://www.caddock.com/Online_catalog/current_sense/current_sense.html over the bottom to evenly contort the heat using a 6 or 12 volt transformer that you should have laying around.---
For more complex solutions here are some options.
I help an entomologist solve the CO2 problem in moving air as show in Perritt,D.W., Baker, R.R., Couger, G. Computer lactometer system for studying behavioral responses of ticks to carbon dioxide. Journal of medical entomology. (ABBREV TITLE = J Med Entomol) May 1993. v. 30 (3)
I believe the specifics on how to build the apparatus and computer program are in the paper. If the particulars an the apparatus aren't in the paper it requires a dual stage regulator for the CO2, $25 dollar 12 volt solenoid valve and a one dollar FET and uses a printer port of MS DOS computer to run it. It is an open loop system that is set by trial and error but it can set the CO2 concentration in air very closely..
I would have made a more outburst solution but it was done for another department and they needed a quick fix and my boss was on my bake about helping others outside the department.
Depending on what kind or computer programing resources you have available. On the crude end a light Bulb and a very small fan blowing over it does a very good job raising the temperature above the ambient temperature with very little over shoot if the bulb is sized right. The fan needs to come on with the light bulb and stay on until it is cooled down to near the temperature of the chamber. I have used power resistors such as these http://www.caddock.com/Online_catalog/current_sense/current_sense.html as heater as well and you can get them in any power dissipation rang and wide range of resistance to use any voltage to make a heater.
If you have no low level computer programming experience available the Tiny 2131 and Plug-in-Bord from http://www.newmicros.com/ using FORT is as easy to get started with as any and they have enough example program to almost make it work.
FORTH is difficult language for big projects but with everything being on board and always ready to go it is very nice for small ones.
I have held the temperature of 1.5 cubic foot box with in 2 degrees with the logic of if the temperature is over 150f turn it off and let the fan run for one minute if the temperature is below 149 turn on the light and wait 10 seconds to turn on the fan. Each of 8 faces of the box had different insulation.
If you need an out of the box solution a look for PID temperature control such as http://www.watlow.com/products/controllers/ and for that small chamber I would use several Caddock resistors http://www.caddock.com/Online_catalog/current_sense/current_sense.html over the bottom to evenly control the heat using a 6 or 12 volt transformer that you should have laying around from the old days.
Gordon Gordon Couger
I collect links on information related to light microscopes. www.couger.com/microscope/links/gclinks.html Please forward anything you think might be useful to others. Microscope Documentation is at www.science-info.org
==============================Original Headers============================== 19, 21 -- From gcc-at-couger.com Fri Oct 28 14:20:37 2005 19, 21 -- Received: from centrmmtao03.cox.net (centrmmtao03.cox.net [70.168.83.81]) 19, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9SJKa05002551 19, 21 -- for {microscopy-at-msa.microscopy.com} ; Fri, 28 Oct 2005 14:20:37 -0500 19, 21 -- Received: from [127.0.0.1] (really [68.12.242.13]) by centrmmtao03.cox.net 19, 21 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 19, 21 -- id {20051028191945.BVLV4942.centrmmtao03.cox.net-at-[127.0.0.1]} ; 19, 21 -- Fri, 28 Oct 2005 15:19:45 -0400 19, 21 -- Message-ID: {43627A05.5090300-at-couger.com} 19, 21 -- Date: Fri, 28 Oct 2005 14:20:37 -0500 19, 21 -- From: Gordon Couger {gcc-at-couger.com} 19, 21 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 19, 21 -- X-Accept-Language: en-us, en 19, 21 -- MIME-Version: 1.0 19, 21 -- To: YANGA-at-AGR.GC.CA, 19, 21 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} 19, 21 -- Subject: Re: [Microscopy] RE: heating live cells 19, 21 -- References: {200510281336.j9SDadlS025924-at-ns.microscopy.com} 19, 21 -- In-Reply-To: {200510281336.j9SDadlS025924-at-ns.microscopy.com} 19, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Indeed, I agree with all of Frank's comments. My earlier answer was too short and Frank addressed several issues I didn't mention. Furthermore, when discussing fluorescence, one has to worry about how well they transmit the wavelength one is interested in. For example, a highly corrected objective may have too much glass or the wrong type of glass to adequately pass UV wavelengths. The ability to pass NIR wavelengths is important in selecting objectives for multiphoton confocal. I also fully agree that objective quality can vary between manufacturers but a comparison between brands N and Z 30 years ago is not especially germane anymore since all the major manufacturers have really changed how they make their objectives in the last 30 years.
At 09:21 AM 10/28/05, you wrote:
} I don't want to start a flame war, I don't wish to lose my access to this } list server...... } } Your statement is only part of the story. Image quality is also affected } by the degree of correction built into the objective. An achromatic } objective may have corrections for one color spherical aberration and two } colors chromatic aberration. An apochromatic would have corrections for } two color spherical aberration and three colors chromatic aberration. I } will not even attempt to fit in flat fields, fluorites, semifluorites and } the old fashion gem lens. With that we still have a series of corrections } for comma, flare, barrel and goodness knows what else. } } So, Given two objectives of the same quality and correction, I would agree } with you, bigger NA means more potential resolving power up to the NA of } the substage condenser as limited by the refractive index of air. (Now } we're into oil immersion systems!) Remember we're are talking about an } optical system of condenser and objective. } } I tell everyone about examining diatoms with two different objectives, one } from company N, the other from company Z (maybe 30 years ago). The two } objectives were the same magnification and had roughly the same NA, but } quality was significantly different. The Z objective made diatom structure } features jump out at me. With the N objective I could find the features } but I had to hunt for them. } } There is more to examining images than resolving power..... } } Frank Karl } Degussa Corporation } Akron Technical Center } 3500 Embassy Parkway } Suite 100 } Akron, Ohio 44333 } } } 330-668-2235 Ext. 238 } } } This e-mail transmission, and any documents, files or previous e-mail } messages attached to it may contain information that is confidential or } legally privileged. If you are not the intended recipient, or a person } responsible for delivering it to the intended recipient, you are hereby } notified that you must not read this transmission and that any disclosure, } copying, printing, distribution or use of any of the information contained } in or attached to this transmission is STRICTLY PROHIBITED. If you have } received this transmission in error, please immediately notify the sender } by telephone or return e-mail and delete the original transmission and its } attachments without reading or saving in any manner. Thank you. } } } } } phillipst-at-missour } } i.edu To: } frank.karl-at-degussa.com } cc: } } 10/28/2005 09:23 Subject: [Microscopy] Re: } LD vs 'regular' LM objectives } AM } } Please respond } to } } phillipst } } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
Thank you for the many useful replies with suggestions on regulating the temperature in our incubator. Of course, off-the-shelf products are great, however, it was astonishing yet reassuring to know that individuals are still willing to spend the time to create novel ways of achieving the same results.
Gratefully yours, Judy
Judy Trogadis Bio-Imaging Coordinator St. Michael's Hospital, 7Queen 30 Bond St. Toronto, ON M5B 1W8 Canada ph: 416-864-6060 x6337
==============================Original Headers============================== 5, 19 -- From trogadisj-at-smh.toronto.on.ca Fri Oct 28 15:03:07 2005 5, 19 -- Received: from smh.toronto.on.ca (mail.smh.toronto.on.ca [199.71.175.103]) 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9SK37sS024035 5, 19 -- for {microscopy-at-msa.microscopy.com} ; Fri, 28 Oct 2005 15:03:07 -0500 5, 19 -- Received: from ([199.71.171.15]) 5, 19 -- by beethoven.smh.toronto.on.ca with ESMTP id KP-GCT47.5469033; 5, 19 -- Fri, 28 Oct 2005 16:02:27 -0400 5, 19 -- Received: from SMTPOUT-MTA by beethoven.smh.toronto.on.ca 5, 19 -- with Novell_GroupWise; Fri, 28 Oct 2005 16:02:27 -0400 5, 19 -- Message-Id: {s3624b93.022-at-beethoven.smh.toronto.on.ca} 5, 19 -- X-Mailer: Novell GroupWise Internet Agent 6.5.2 5, 19 -- Date: Fri, 28 Oct 2005 16:01:55 -0400 5, 19 -- From: "Judy Trogadis" {TrogadisJ-at-smh.toronto.on.ca} 5, 19 -- To: {Microscopy-at-msa.microscopy.com} 5, 19 -- Subject: live cell heating device 5, 19 -- Mime-Version: 1.0 5, 19 -- Content-Type: text/plain; charset=US-ASCII 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- Content-Disposition: inline ==============================End of - Headers==============================
Dear Listmembers, I need to order a new microtome primarily for sectioning methacrylate-embedded tissue for light microscopy. The choice will probably be between the Microm HM-355C and an RMC MT-990, with possibly a Leica RM2255 also being a candidate. I know almost nothing about the Microm, and absolutely nothing about the RMC. If any of you have used either of these products or have any firm opinions of them I would really like to hear from you. This probably isn't of great interest to most people, so perhaps offline to me at david.griffiths-at-veths.no is best.
Thankyou, David Griffiths
David Griffiths Section of Anatomy and Pathology Norwegian School of Veterinary Science P. O. Box 8146 Dep N-0033 Oslo Norway E-mail: david.griffiths-at-veths.no
------------------------------------------------------------------------------- This verifies that this e-mail has been scanned for virus and deemed virus-free according to Bitdefender and Norman scan engines. 28/10/2005 -------------------------------------------------------------------------------
==============================Original Headers============================== 9, 23 -- From David.Griffiths-at-veths.no Fri Oct 28 16:33:18 2005 9, 23 -- Received: from nvhmail.veths.no (firewall.veths.no [158.36.103.2]) 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9SLXHQn001122 9, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Oct 2005 16:33:18 -0500 9, 23 -- Received: from NVHMAIL.veths.no ([10.77.17.50]) by nvhmail.veths.no with Microsoft SMTPSVC(6.0.3790.1830); Fri, 28 Oct 2005 23:33:32 +0200 9, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.1830 9, 23 -- Content-Class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="iso-8859-1" 9, 23 -- Subject: LM_Microtomes_HPMA_Methacrylates_Recommendations 9, 23 -- Date: Fri, 28 Oct 2005 23:33:16 +0200 9, 23 -- Message-ID: {25C5D665E2C618418545AEEC83FA0C341D8D78-at-nvhmail.veths.no} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: LM_Microtomes_HPMA_Methacrylates_Recommendations 9, 23 -- thread-index: AcXcBzUGhWEX3l6yS7GbcogOo3Ku3w== 9, 23 -- From: "Griffiths David" {David.Griffiths-at-veths.no} 9, 23 -- To: {Microscopy-at-microscopy.com} 9, 23 -- CC: {daveg-at-online.no} 9, 23 -- X-OriginalArrivalTime: 28 Oct 2005 21:33:32.0906 (UTC) FILETIME=[3EF51CA0:01C5DC07] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9SLXHQn001122 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jmastrangelo-at-ulbi.com) from http://www.microscopy.com/MLFormMail.html on Friday, October 28, 2005 at 08:54:33 ---------------------------------------------------------------------------
Email: jmastrangelo-at-ulbi.com Name: Joseph Mastrangelo
Organization: Ultralife Batteries
Title-Subject: [Filtered] Mn / F resolution with EDS
Question: Good morning everyone,
We have recently purchased an SEM/EDS system for the purpose of materials qualification. The system seems to be working fine, but we are having some difficulty when attempting to analyze fluorine in samples that contain high amounts of manganese. We assume this difficulty is due to the ROI overlap for Mn and F. We do realize that the EDS system is semi-quantitative at best, but the overlap seems to be causing the Fluorine content to be magnified by a factor of 3 or 4 in the analysis. Just wondering if anyone out there has had any similar experiences, and whether there are any "tricks of the trade" for dealing with this.
Thanks in advance for your input,
Joe Mastrangelo Chemical Lab Technician Ultralife Batteries Newark, NY jmastrangelo-at-ulbi.com
To get that level of compliance you would have to implant RFID chips in the users hands and only activate the scope when it senses and ID tag in the area and register the user.
You should be able to get a satisfactory level of compliance from professionals and student with an ID chip in a bracelet, ring. watch band, active ID badge or card in a passive system. The RFID sensor registers, records and turns on the instrument if the user has privileges for it from who ever's RFID is in the capture area of the scope. Here is one example I found with Google search "RFID card" SDK http://www.geometrix.com/solutions/access.html
Be careful in this area you are very close to the system being put in track cattle from conception to the fork so be careful how you present the idea on bad joke can send it down the tubes.
I expect the RFID solution would be more expensive than the card swipe but the RFID should work almost every time logging users out and catching the change of users with in a few minuets of the actual change. I expect you will be lucky to get 40% log off compliance with a card swipe. I know I would forget a good part of the time.
I am an inactive part owner of a company that develops this kind of solution as well as microscope hobbyist. We have done work in this area but have not done any projects.
Gordon Couger DataLink Systems www.rfdata.net
opmills-at-mtu.edu wrote:
} } Hi, } } I've been alerted that funding may be available for the installation } of magnetic strip card readers to tracking instrument use in my } facility. Apparently they are being used successfully for this } purpose in a university micro-fabrication facility in our state. If } they work, perhaps it would simplify the accounting involved in } monthly invoicing. However, I have a couple of concerns about using } them in our facility that I can't work out. Maybe you can help. } } Do you install the reader on the instrument itself, or on the doorway } of the lab. This is the standard way of installing readers at my } university. There is a problem when they are used on a lab door when } there are several instruments inside. } } Can readers be interlocked into the instruments to prevent use until } a card is swiped? Has anyone done that? How? } } If I use a swipe style reader, we could probably get compliance when } a user gets started. However, we'd have to get them to swipe again } at the end of their use to capture the elapsed time (which is what we } really want!). If they don't swipe again at the end of their } session, I'll have to spend more time determining how much time they } used. } } Have any of you used a card reader like the ones on ATM machines that } actually "keep" your card until the transaction is completed? A } reader like this might solve the problem above. } } There is a problem for automated instruments (like microprobes) where } a sample run may extend into the early morning hours. Since no one } will be there to swipe at the end, how could we accurately track use } in those situations? } } I appreciate any input you can offer. Thanks! } } Owen } } Owen P. Mills } Director, Materials Characterization and Fabrication Facilities } Electron Optics Engineer, } Applied Chemical & Morphological Analysis Laboratory } Materials Science & Engineering } Michigan Technological University } Rm 512 M&M Bldg. } Houghton, MI 49931 } PH 906-369-1875 } FAX 906-487-2934 } mailto:opmills-at-mtu.edu } http://www.mm.mtu.edu/~opmills } } } } } Owen P. Mills } Director, Materials Characterization and Fabrication Facilities } Electron Optics Engineer, } Applied Chemical & Morphological Analysis Laboratory } Materials Science & Engineering } Michigan Technological University } Rm 512 M&M Bldg. } Houghton, MI 49931 } PH 906-369-1875 } FAX 906-487-2934 } mailto:opmills-at-mtu.edu } http://www.mm.mtu.edu/~opmills }
}
==============================Original Headers============================== 15, 21 -- From gcc-at-couger.com Fri Oct 28 21:26:52 2005 15, 21 -- Received: from centrmmtao06.cox.net (centrmmtao06.cox.net [70.168.83.78]) 15, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9T2Qpkg021278 15, 21 -- for {microscopy-at-msa.microscopy.com} ; Fri, 28 Oct 2005 21:26:51 -0500 15, 21 -- Received: from [127.0.0.1] (really [68.12.242.13]) by centrmmtao06.cox.net 15, 21 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 15, 21 -- id {20051029022609.BZKD24602.centrmmtao06.cox.net-at-[127.0.0.1]} ; 15, 21 -- Fri, 28 Oct 2005 22:26:09 -0400 15, 21 -- Message-ID: {4362DDEA.2000200-at-couger.com} 15, 21 -- Date: Fri, 28 Oct 2005 21:26:50 -0500 15, 21 -- From: Gordon Couger {gcc-at-couger.com} 15, 21 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 15, 21 -- X-Accept-Language: en-us, en 15, 21 -- MIME-Version: 1.0 15, 21 -- To: opmills-at-mtu.edu, 15, 21 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} 15, 21 -- Subject: Re: [Microscopy] Magnetic card readers for instrument access 15, 21 -- References: {200510281920.j9SJKCoh002237-at-ns.microscopy.com} 15, 21 -- In-Reply-To: {200510281920.j9SJKCoh002237-at-ns.microscopy.com} 15, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
} Hi Jon, } } We only have one Zeiss 'air' LD plan neofluar - a 63x. However it's image } quality is noticeably poorer than our Zeiss oil immersion 63x Plan } Apochromat's, using confocal and standard fluorescence imaging } configurations. The LD objective was over £1,000 cheaper - we use it for } both epi-fluorescence and Ph transmission. } } Our LD obviously scores if you want to image something 1mm into the sample } though owing to its very long working distance (WD 1.2 to 2.2 mm). In } comparison our Plan Apochromat high power oil objectives (WD = 0.19 mm) only } just get into the cells stuck onto the coverslip (e.g. using Mattek dishes) } and we can't see anything at all with a slightly raised coverslip on slides. } } On our Leica SP2 AOBS confocal system, our 'blue' Leica 20x HCL PL APO } water/glycerol/oil objective has very good image quality and a slightly } bigger WD with water compared to oil immersion (free WD = 0.26 mm water or } 0.17 mm oil). However water immersion is a pain with the inverted } microscopes we use. } } Our local Zeiss rep is always happy to lend us objectives for appraisal, } which is extremely useful. } } Regards } } Keith } } PS. Years ago at a meeting I heard that higher NA increases the resolution, } but lowering the NA (assuming you have a variable NA collar on your } objective) improves the contrast, which might be more useful in some cases. } It seems to work with the one variable NA objective we have. } Keith,
Your pretty much right but the n.a. included the coder and the lower the n.a. the larger the depth of field. In my mind I believe that is stating the same thing in two different ways. But its ben 44 years since I had physics.
I have a Leitz 63x .83 n.a. with a variable aperture it is the companion to their dry dark field condensers and everything you say about the effect of n.a. is pretty much on the money. The iris can be closed to the point on the Leitz 63x that n.a. is so small that the image degradation due to detraction on the edge of the blades of the iris is noticeable.
Gordon Gordon Couger
I collect links on information related to light microscopes. www.couger.com/microscope/links/gclinks.html Please forward anything you think might be useful to others. Microscope Documentation is at www.science-info.org
==============================Original Headers============================== 10, 22 -- From gcc-at-couger.com Sat Oct 29 00:40:24 2005 10, 22 -- Received: from centrmmtao06.cox.net (centrmmtao06.cox.net [70.168.83.78]) 10, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9T5eNVe031804 10, 22 -- for {microscopy-at-msa.microscopy.com} ; Sat, 29 Oct 2005 00:40:24 -0500 10, 22 -- Received: from [127.0.0.1] (really [68.12.242.13]) by centrmmtao06.cox.net 10, 22 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 10, 22 -- id {20051029053941.DKQZ24602.centrmmtao06.cox.net-at-[127.0.0.1]} ; 10, 22 -- Sat, 29 Oct 2005 01:39:41 -0400 10, 22 -- Message-ID: {43630B4A.4040500-at-couger.com} 10, 22 -- Date: Sat, 29 Oct 2005 00:40:26 -0500 10, 22 -- From: Gordon Couger {gcc-at-couger.com} 10, 22 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 10, 22 -- X-Accept-Language: en-us, en 10, 22 -- MIME-Version: 1.0 10, 22 -- To: keith.morris-at-ucl.ac.uk, 10, 22 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} 10, 22 -- Subject: Re: [Microscopy] Re: LD vs 'regular' LM objectives 10, 22 -- References: {200510281454.j9SEsPQH028772-at-ns.microscopy.com} 10, 22 -- In-Reply-To: {200510281454.j9SEsPQH028772-at-ns.microscopy.com} 10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 22 -- Content-Transfer-Encoding: 8bit 10, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9T5eNVe031804 ==============================End of - Headers==============================
We have 4 dipping objectives (no coverslip needed) with about 2 mm WD, as well as high NA, short WD water immersion objectives. We use the dipping objectives on the upright microscope when we need to do microinjection or change incubation solution or look at large, odd-shaped specimens, etc. I am impressed at how good they are. The 63x dipping objective, with NA 0.9, is very bright (for fluorescence) and quite good resolution, though you can clearly see the superior resolution and brightness of the NA 1.2 water immersion objective. Since the latter cost about twice as much as the former, you'd want to see a difference in performance.....
Rosemary
Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 02-6246 5475 GPO Box 1600 mob. 0402 835 973 Canberra, ACT 2601 fax. 02-6246 5334 Australia
} From: gcc-at-couger.com } Reply-To: gcc-at-couger.com } Date: Sat, 29 Oct 2005 00:44:29 -0500 } To: rosemary.white-at-csiro.au } Subject: [Microscopy] LD vs 'regular' LM objectives } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } Hi Jon, } } } } We only have one Zeiss 'air' LD plan neofluar - a 63x. However it's image } } quality is noticeably poorer than our Zeiss oil immersion 63x Plan } } Apochromat's, using confocal and standard fluorescence imaging } } configurations. The LD objective was over £1,000 cheaper - we use it for } } both epi-fluorescence and Ph transmission. } } } } Our LD obviously scores if you want to image something 1mm into the sample } } though owing to its very long working distance (WD 1.2 to 2.2 mm). In } } comparison our Plan Apochromat high power oil objectives (WD = 0.19 mm) } only } } just get into the cells stuck onto the coverslip (e.g. using Mattek } dishes) } } and we can't see anything at all with a slightly raised coverslip on } slides. } } } } On our Leica SP2 AOBS confocal system, our 'blue' Leica 20x HCL PL APO } } water/glycerol/oil objective has very good image quality and a slightly } } bigger WD with water compared to oil immersion (free WD = 0.26 mm water or } } 0.17 mm oil). However water immersion is a pain with the inverted } } microscopes we use. } } } } Our local Zeiss rep is always happy to lend us objectives for appraisal, } } which is extremely useful. } } } } Regards } } } } Keith } } } } PS. Years ago at a meeting I heard that higher NA increases the } resolution, } } but lowering the NA (assuming you have a variable NA collar on your } } objective) improves the contrast, which might be more useful in some } cases. } } It seems to work with the one variable NA objective we have. } } } Keith, } } Your pretty much right but the n.a. included the coder and the lower the } n.a. the larger the depth of field. In my mind I believe that is stating } the same thing in two different ways. But its ben 44 years since I had } physics. } } I have a Leitz 63x .83 n.a. with a variable aperture it is the companion } to their dry dark field condensers and everything you say about the effect } of n.a. is pretty much on the money. The iris can be closed to the point } on the Leitz 63x that n.a. is so small that the image degradation due to } detraction on the edge of the blades of the iris is noticeable. } } Gordon } Gordon Couger } } I collect links on information related to light microscopes. } www.couger.com/microscope/links/gclinks.html } Please forward anything you think might be useful to others. } Microscope Documentation is at www.science-info.org } } } } } } ==============================Original Headers============================== } 10, 22 -- From gcc-at-couger.com Sat Oct 29 00:40:24 2005 } 10, 22 -- Received: from centrmmtao06.cox.net (centrmmtao06.cox.net } [70.168.83.78]) } 10, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9T5eNVe031804 } 10, 22 -- for {microscopy-at-msa.microscopy.com} ; Sat, 29 Oct 2005 00:40:24 } -0500 } 10, 22 -- Received: from [127.0.0.1] (really [68.12.242.13]) by } centrmmtao06.cox.net } 10, 22 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with } ESMTP } 10, 22 -- id } {20051029053941.DKQZ24602.centrmmtao06.cox.net-at-[127.0.0.1]} ; } 10, 22 -- Sat, 29 Oct 2005 01:39:41 -0400 } 10, 22 -- Message-ID: {43630B4A.4040500-at-couger.com} } 10, 22 -- Date: Sat, 29 Oct 2005 00:40:26 -0500 } 10, 22 -- From: Gordon Couger {gcc-at-couger.com} } 10, 22 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) } 10, 22 -- X-Accept-Language: en-us, en } 10, 22 -- MIME-Version: 1.0 } 10, 22 -- To: keith.morris-at-ucl.ac.uk, } 10, 22 -- "microscopy-at-msa.microscopy.com" } {microscopy-at-msa.microscopy.com} } 10, 22 -- Subject: Re: [Microscopy] Re: LD vs 'regular' LM objectives } 10, 22 -- References: {200510281454.j9SEsPQH028772-at-ns.microscopy.com} } 10, 22 -- In-Reply-To: {200510281454.j9SEsPQH028772-at-ns.microscopy.com} } 10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 10, 22 -- Content-Transfer-Encoding: 8bit } 10, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id j9T5eNVe031804 } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 22 -- From Rosemary.White-at-csiro.au Sat Oct 29 02:25:15 2005 7, 22 -- Received: from vic-ironport-ext-out1.csiro.au (vic-ironport-ext-out1.csiro.au [150.229.64.37]) 7, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9T7PEmY008918 7, 22 -- for {Microscopy-at-microscopy.com} ; Sat, 29 Oct 2005 02:25:14 -0500 7, 22 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 7, 22 -- by vic-ironport-ext-out1.csiro.au with ESMTP; 29 Oct 2005 17:25:14 +1000 7, 22 -- X-IronPort-AV: i="3.97,265,1125842400"; 7, 22 -- d="scan'208"; a="56512764:sNHT25943836" 7, 22 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 7, 22 -- Sat, 29 Oct 2005 17:25:12 +1000 7, 22 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 7, 22 -- Date: Sat, 29 Oct 2005 17:26:44 +1000 7, 22 -- Subject: Re: [Microscopy] LD vs 'regular' LM objectives 7, 22 -- From: Rosemary White {Rosemary.White-at-csiro.au} 7, 22 -- To: {Microscopy-at-microscopy.com} 7, 22 -- Message-ID: {BF896154.12CD6%Rosemary.White-at-csiro.au} 7, 22 -- In-Reply-To: {200510290544.j9T5iTDT004250-at-ns.microscopy.com} 7, 22 -- Mime-version: 1.0 7, 22 -- Content-type: text/plain; charset="ISO-8859-1" 7, 22 -- X-OriginalArrivalTime: 29 Oct 2005 07:25:12.0771 (UTC) FILETIME=[E686A130:01C5DC59] 7, 22 -- Content-Transfer-Encoding: 8bit 7, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id j9T7PEmY008918 ==============================End of - Headers==============================
The WWW site for the Microscopy & Microanalysis 2006 Meeting in Chicago July 30-August 3 has been recently updated with the full meeting details.
http://mm2006.microscopy.org
Please feel free to visit this site at your convenience and to share this information with colleagues and students who may not be members of this Listserver community.
While a few of the on-line registration/submission pages are still in the process of being established, the all of the information concerning the meeting as well as Pre-Meeting Congress and Sunday Short Courses is now on-line.
A hard copy of the Call for Papers is currently in press and will be mailed shortly to all sponsoring society members as well as recent meeting participants.
Hope to see you (all) in Chicago, as by some strange coincidence it appears I will also be there.
Nestor J. Zaluzec Your Friendly Neighborhood SysOp & MM2006 Local Arrangement Committee Co-Chair
==============================Original Headers============================== 9, 11 -- From zaluzec-at-microscopy.com Sat Oct 29 12:19:18 2005 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 9, 11 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9THJGOi024182; 9, 11 -- Sat, 29 Oct 2005 12:19:16 -0500 9, 11 -- Mime-Version: 1.0 9, 11 -- Message-Id: {p06110406bf894b954a6d-at-[206.69.208.22]} 9, 11 -- Date: Sat, 29 Oct 2005 12:19:16 -0500 9, 11 -- To: microscopy-at-microscopy.com, maslistserver-at-microprobe.org 9, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 9, 11 -- Subject: Microscopy & Microanalysis 2006 9, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
the overlap of F between several Mn-L lines is very strong and a real challenge for each deconvolution procedure. The deconvolution software must know all line positions very well and the distortions of lines (detector dependance). But the major problem is not to deconvolute the net count rates of all lines in this spectra region, but the strong absorption of F in Manganese due to the Mn-L3 and L2 absorption jumps. This should be the main problem for quantitative analysis, not only for an EDS.
Look to some numbers: If the F is emitted in 0.1 micron depth of specimen, only about 19 per cent of X-rays of 677 eV (F-Ka) are able to leave the specimen (the opposite is absorbed). Nothing is coming from 0.3 micron depth. Only a little change in energy to a lower X-ray energy (630 eV) and 55% are coming out from 0.3 micron and 82 per cent from 0.1.
This is the real problem, your quantitative software is faced to. The absorption is extremly strong. Therefore the errors and fluctuations you must expect in quantitative results are much higher than with other analytical situations. Finally the absolute deviations of your results depends of the mass absorption coefficients your software is using. These coefficients are badly well-known around absorption edges and still more badly in low energy regions (below 1 keV). If your system magnify the Fluorine content with factor 3..4 with samples of high Manganese contents, the mass absorption coefficient of F in Mn must be simply too high. But take in mind, the results fluctuations you have to expect are still present, even if you will able to adjust the coefficient (due to the always strong absorption).
You can have a look to the line energies, absorption jumps, mass absorption coefficients (MAC's) and the absorption of X-rays in a given layer with MA-Table program:
http://www.microanalyst.net/registr.phtml and http://www.microanalyst.net/manual.html
Best regards
Frank Eggert
jmastrangelo-at-ulbi.com wrote:
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==============================Original Headers============================== 11, 19 -- From eggert-at-mikroanalytik.de Mon Oct 31 00:24:08 2005 11, 19 -- Received: from Mail1.KONTENT.De (mail1.kontent.de [81.88.34.36]) 11, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9V6O7X2031985 11, 19 -- for {microscopy-at-microscopy.com} ; Mon, 31 Oct 2005 00:24:08 -0600 11, 19 -- Received: from mikroanalytik.de (p54BDDCD1.dip.t-dialin.net [84.189.220.209]) 11, 19 -- by Mail1.KONTENT.De (Postfix) with ESMTP id 70303105607C; 11, 19 -- Mon, 31 Oct 2005 07:24:08 +0100 (CET) 11, 19 -- Message-ID: {4365BA3E.70505-at-mikroanalytik.de} 11, 19 -- Date: Mon, 31 Oct 2005 07:31:26 +0100 11, 19 -- From: Frank Eggert {eggert-at-mikroanalytik.de} 11, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; de-DE; rv:1.4) Gecko/20030619 Netscape/7.1 (ax) 11, 19 -- X-Accept-Language: de, de-de, en 11, 19 -- MIME-Version: 1.0 11, 19 -- To: jmastrangelo-at-ulbi.com, microscopy-at-microscopy.com 11, 19 -- Subject: Re: [Microscopy] viaWWW: Mn / F analysis with EDS 11, 19 -- References: {200510282247.j9SMlgve014319-at-ns.microscopy.com} 11, 19 -- In-Reply-To: {200510282247.j9SMlgve014319-at-ns.microscopy.com} 11, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We are looking for some great ideas for symposia at M&M2007!
Microscopy & Microanalysis 2007 Meeting
August 6-9, 2007 Broward County Convention Center Ft. Lauderdale, Florida
Co-sponsored by
The Microscopy Society of America The Microbeam Analysis Society The International Metallographic Society
Although we have suggestions for some of the customary symposia, and have already signed on a small number of organizers, the program is largely open at this time.
We would like the majority of the proposals to be submitted by the end of the year. We will start sending out acceptance letters in late December, and by mid-February we expect the program to be mostly filled.
This timetable is considerably accelerated in comparison with previous years, and we now require a description of 150-300 words for each proposed symposia. The description should take the form of those found in the Call for Papers and Expo of past years; it should be an announcement of the symposium and an invitation for contributions. The Program Committee will select symposia based on these descriptions, so that overlap will be minimized and symposia will complement each other to form a coherent overall program.
You need not be a member of MSA, MAS, or IMS to propose a symposium, although we hope that your experience with the M&M meeting will encourage you to join.
Please send your suggestion (complete with description) directly to the Program Chair, or to the M&M2007 Co-Chair of your Society.
The M&M2007 website is
http://mm2007.microscopy.org/
The site can be reached by many right now, and contains the same information as in this email. It will be available to all by the end of next week. Near the end of the year, accepted symposia will be posted on the website.
Program contacts:
Mike Marko, Program Chair (marko-at-wadsworth.org) John Henry Scott, Vice Program Chair (johnhenry.scott-at-nist.gov) Ed Vincenzi, MAS Program Co-Chair (vicenzi-at-volcano.si.edu) Steve Dekanich, IMS Program Co-Chair (dekanichsj-at-y12.doe.gov)
Local Arrangments contact:
Lucille Gianuzzi, Local Arrangements Chair (lgiannuzzi-at-feico.com)
Meeting Management contact:
Phillip Ridley, Conference Manager Bostrom Corp 230 East Ohio, Suite 400 Chicago, IL 60611 Tel: 312-644-0828 Fax: 312-644-8557 Email: pridley-at-bostrom.com
==============================Original Headers============================== 22, 22 -- From marko-at-wadsworth.org Mon Oct 31 09:38:06 2005 22, 22 -- Received: from mailserv.wadsworth.org (mailserv.wadsworth.org [199.184.30.43]) 22, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9VFc6UM015214 22, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 31 Oct 2005 09:38:06 -0600 22, 22 -- Received: from jinmen (pasteur-0253 [172.16.11.253]) 22, 22 -- by mailserv.wadsworth.org (8.12.10+Sun/8.12.10) with SMTP id j9VFc3eZ026271 22, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 31 Oct 2005 10:38:03 -0500 (EST) 22, 22 -- Message-Id: {3.0.6.32.20051031105958.00a286f0-at-pop3s.wadsworth.org} 22, 22 -- X-Sender: marko-at-pop3s.wadsworth.org 22, 22 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 22, 22 -- Date: Mon, 31 Oct 2005 10:59:58 -0800 22, 22 -- To: Microscopy-at-microscopy.com 22, 22 -- From: Michael Marko {marko-at-wadsworth.org} 22, 22 -- Subject: Invitation to organize symposia for M&M2007 22, 22 -- Mime-Version: 1.0 22, 22 -- Content-Type: text/plain; charset="us-ascii" 22, 22 -- X-Wadsworth-MailScanner-Information: Please contact CSS for more information 22, 22 -- X-Wadsworth-MailScanner: Found to be clean 22, 22 -- X-Wadsworth-MailScanner-SpamCheck: not spam, SpamAssassin (score=-101.06, 22, 22 -- required 5, AWL -0.42, BAYES_00 -2.60, DATE_IN_FUTURE_03_06 1.96, 22, 22 -- USER_IN_WHITELIST -100.00) 22, 22 -- X-MailScanner-From: marko-at-wadsworth.org ==============================End of - Headers==============================
This Question was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both KOCHINST-at-GLOBO.COM as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: KOCHINST-at-GLOBO.COM Name: ROBERT KOCH
Organization: KOCH INSTRUMENTOS CIENTÕFICOS LTDA.
Title-Subject: [Filtered] MListserver:
Question: Dear Microscopists,
we would like to know, if someone has information about the very first freezing microtome.
Thank you
Robert Koch
Koch instrumentos CientÌficos Ltda. Rua Joaquim Nabuco, 655 S“o Paulo - SP - Brazil 55-11-5092-4622 tel/fax kochinst-at-globo.com www.kochinst.com.br