All welcome but registration forms should be FAXed to Will Maxwell at 0141 330 4299 in order to assess numbers for catering. This can be followed by payment made on arrival.
Dr Laurence Tetley Division of Infection & Immunity, IBLS, Integrated Microscopy Facility Joseph Black Building University of Glasgow Glasgow G12 8QQ
l.tetley-at-bio.gla.ac.uk tel 0141 330 4431 FAX 0141 330 3516
Here is the November 2005 Microscopy Today table of contents. I will close the subscription list for this issue on Monday, November 7, 2005.
Microscopists in North America and MSA members anywhere may have free subscriptions. Anyone else may subscribe for US$35 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com Thank you. Non-Qualified subscription rates will increase to US$50 in 2006.
Ron Anderson, Editor ==================================
Very Cool Clathrin Stephen W. Carmichael, Mayo Clinic
Metallography for the European Copper Age: Research on the Axe-Blade of the Glacier-Mummy from the Ötztaler Alps in Tyrol Gerhard O. Sperl, Institute for Historical Materials, Leoben, Austria
Microscopy and Microbes at Plum Island: Protecting America’s Livestock Thomas G. Burrage, Plum Island Animal Disease Center, NY
Color Metallography George F. Vander Voort, Buehler Ltd, Lake Bluff, Illinois
Characterization of Solids from Oilfield Emulsions Richard W. Cloud,† Rebecca L. Ramsey,‡ Robert A. Pultz,‡ and Michael K. Poindexter‡, † Nalco Company, Naperville, Illinois; ‡ Nalco Energy Services, Sugar Land, Texas
Automated, Robotic Preparation of Vitrified Samples for 2D and 3D Cryo Electron Microscopy P. M. Frederik1 and M.H. Storms2; 1Univ. Maastricht, The Netherlands, 2. FEI Company, Achtseweg Noord Eindhoven, The Netherlands
Ex-Situ “Auto Lift” Technique for TEM Sample Preparation Garth “Brian” Cook, Micro Optics of Florida, Davie, Florida
Writing Nano-Scale Patterns on Insulators Using Variable Pressure Electron-Beam Lithography Floyd Miller and David Frey,* Lehigh University Bethlehem, PA and *Carl Zeiss SMT Inc . Microscopy for Children Caroline Schooley, MSA Project MICRO
Fostering LIMS Development Through Open Standards Avrum Goodblatt, U. Penn School of Medicine, Philadelphia
50th Annivsary Celebrations of Atomic-Resolution Imaging Thomas F. Kelly and Allan J. Melmed
Attaching Spheres to Cantilevers for Colloidal Probe Force Measurements: A Simplified Technique Yang Gan, University of Newcastle, Callaghan, NSW, Australia
Nissl: The Man, The Stain and The Substance John A. Kiernan, The Univ. of Western Ontario London, Canada
Industry News NetNotes Index of Advertisers
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This Question was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wgunning-at-meduohio.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wgunning-at-meduohio.edu Name: WT Gunning
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Title-Subject: [Filtered] MListserver: MSA Award Nominations Reminder
Question: Please note that MSA Awards nominations are due on December 15th, 2005.
2006 MICROSCOPY SOCIETY OF AMERICA AWARDS
The Distinguished Scientist Awards (Physical & Biological Sciences)
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Question: We are approaching an abstract deadline for the Spring Experimental Biology 2006 Meeting in San Francisco April 1-5, 2006 (a national meeting with an attendance of about 15,000 people, see FASEBís website www.faseb.org). There is a platform session entitled ìElectron Microscopy as a 21st Century Toolî with a 15 minute talks. This platform session is entitled ìElectron Microscopy as a 21st Century Toolî and sponsored by the American Association of Anatomists-AAA and is being supported financially, in part, by the Indiana Microscopy Society and a request support from the MSA will be submitted. If you are interested in presenting some of your cutting edge, biological EM data, please consider submitting an abstract for the session. You will need to submit an abstract (the website URL for submission of an abstract is below). I will try to get sufficient funding so that all who present in this session can get reimbursed for the abstract fee as well as your registration and other associated expenses (hopefully) after the meeting. Make sure you submit for the AAA platform session entitled ìElectron Microscopy as a 21st Century Toolî.
Abstract submission deadline is November 7, 2005 . Thank you for considering submitting for this meeting
This Question was submitted to Ask-A-Microscopist by (Willem.Wennekes-at-comcast.net) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, November 1, 2005 at 21:49:12 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both Willem.Wennekes-at-comcast.net as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: Willem.Wennekes-at-comcast.net Name: Willem Wennekes
Organization: UES
Education: Graduate College
Location: Dayton, OH, USA
Title: edx
Question: Hi, What would be the benefit of using a larger objective apperture instead of increasing the probe current in order to increase the beam current for edx analysis and x-ray mapping?
} } Email: Willem.Wennekes-at-comcast.net } Name: Willem Wennekes } } Organization: UES } } Education: Graduate College } } Location: Dayton, OH, USA } } Title: edx } } Question: Hi, What would be the benefit of using a larger } objective apperture instead of increasing the probe current in order } to increase the beam current for edx analysis and x-ray mapping? } } Cheers, } Willem
The benefit is that you keep your small aperture clean for imaging.
==============================Original Headers============================== 2, 35 -- From David.R.Hull-at-nasa.gov Wed Nov 2 10:21:26 2005 2, 35 -- Received: from mx1.grc.nasa.gov (mx1.grc.nasa.gov [128.156.11.68]) 2, 35 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA2GLPtL024202 2, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Nov 2005 10:21:26 -0600 2, 35 -- Received: from lombok-fi.grc.nasa.gov (seraph4.grc.nasa.gov [128.156.10.13]) 2, 35 -- by mx1.grc.nasa.gov (Postfix) with ESMTP id 226C9C2BC 2, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Nov 2005 11:21:21 -0500 (EST) 2, 35 -- Received: from apataki.grc.nasa.gov (apataki.grc.nasa.gov [139.88.112.35]) 2, 35 -- by lombok-fi.grc.nasa.gov (NASA GRC TCPD 8.12.10/8.12.10) with ESMTP id jA2GLKGT021928; 2, 35 -- Wed, 2 Nov 2005 11:21:20 -0500 (EST) 2, 35 -- Received: from apataki.grc.nasa.gov (localhost [127.0.0.1]) 2, 35 -- by apataki.grc.nasa.gov (NASA GRC TCPD 8.13.1/8.13.1) with ESMTP id jA2GLJQG015282; 2, 35 -- Wed, 2 Nov 2005 11:21:20 -0500 (EST) 2, 35 -- Received: from manihi.grc.nasa.gov (manihi.grc.nasa.gov [139.88.112.36])by 2, 35 -- apataki.grc.nasa.gov (NASA GRC TCPD 8.13.1/8.13.1) with ESMTP id 2, 35 -- jA2GLIfr015268;Wed, 2 Nov 2005 11:21:18 -0500 (EST) 2, 35 -- Received: from [139.88.183.36] (gr000931123.grc.nasa.gov [139.88.183.36])by 2, 35 -- manihi.grc.nasa.gov (NASA GRC TCPD 8.12.10/8.12.10) with ESMTP id 2, 35 -- jA2GLHsi024829;Wed, 2 Nov 2005 11:21:17 -0500 (EST) 2, 35 -- Mime-Version: 1.0 2, 35 -- X-Sender: mshull-at-popserve.grc.nasa.gov 2, 35 -- Message-Id: {p06100525bf8e861b5fea-at-[139.88.183.36]} 2, 35 -- In-Reply-To: {200511021344.jA2DiqDn013958-at-ns.microscopy.com} 2, 35 -- References: {200511021344.jA2DiqDn013958-at-ns.microscopy.com} 2, 35 -- Date: Wed, 2 Nov 2005 11:21:16 -0400 2, 35 -- To: Willem.Wennekes-at-comcast.net, Microscopy-at-microscopy.com 2, 35 -- From: David R Hull {David.R.Hull-at-nasa.gov} 2, 35 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: Aperture vs Currentfor EDX analysis 2, 35 -- Content-Type: text/plain; 2, 35 -- charset=us-ascii; 2, 35 -- format=flowed 2, 35 -- X-imss-version: 2.033 2, 35 -- X-imss-result: Passed 2, 35 -- X-imss-scores: Clean:80.37382 C:2 M:3 S:5 R:5 2, 35 -- X-imss-settings: Baseline:1 C:1 M:2 S:2 R:2 (0.0000 0.0000) ==============================End of - Headers==============================
Willem, When you increase your probe size (demagnify the source less), the spray electrons from each crossover tend to be captured closer to the beam path thereby creating heavier contamination buildup nearer to the beam. This can result in faster degradation of your imaging capabilities and the need for more frequent cleaning of the column.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: Willem.Wennekes-at-comcast.net [mailto:Willem.Wennekes-at-comcast.net] Sent: Wednesday, November 02, 2005 8:47 AM To: kenconverse-at-qualityimages.biz
This Question was submitted to Ask-A-Microscopist by (Willem.Wennekes-at-comcast.net) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, November 1, 2005 at 21:49:12 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both Willem.Wennekes-at-comcast.net as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: Willem.Wennekes-at-comcast.net Name: Willem Wennekes
Organization: UES
Education: Graduate College
Location: Dayton, OH, USA
Title: edx
Question: Hi, What would be the benefit of using a larger objective apperture instead of increasing the probe current in order to increase the beam current for edx analysis and x-ray mapping?
==============================Original Headers============================== 9, 12 -- From zaluzec-at-ultra5.microscopy.com Wed Nov 2 07:44:18 2005 9, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 9, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA2DiH73012579 9, 12 -- for {microscopy-at-microscopy.com} ; Wed, 2 Nov 2005 07:44:17 -0600 9, 12 -- Mime-Version: 1.0 9, 12 -- X-Sender: zaluzec-at-ultra5.microscopy.com (Unverified) 9, 12 -- Message-Id: {p06110406bf8e730200cf-at-[206.69.208.22]} 9, 12 -- Date: Wed, 2 Nov 2005 07:44:17 -0600 9, 12 -- To: microscopy-at-microscopy.com 9, 12 -- From: Willem.Wennekes-at-comcast.net (by way of Ask-A-Microscopist) 9, 12 -- Subject: [Filtered] AskAMicroscopist: Aperture vs Current for EDX analysis 9, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
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==============================Original Headers============================== 25, 24 -- From kenconverse-at-qualityimages.biz Wed Nov 2 11:01:30 2005 25, 24 -- Received: from qualityimages.biz (dpmail16.doteasy.com [65.61.209.16]) 25, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA2H1TZg001283 25, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 2 Nov 2005 11:01:30 -0600 25, 24 -- Received: from Ken [68.64.242.101] by qualityimages.biz with ESMTP 25, 24 -- (SMTPD32-8.05) id A06D1B510084; Wed, 02 Nov 2005 08:59:25 -0800 25, 24 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 25, 24 -- To: {Willem.Wennekes-at-comcast.net} , 25, 24 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 25, 24 -- Subject: RE: [Microscopy] [Filtered] AskAMicroscopist: Aperture vs Current for EDX analysis 25, 24 -- Date: Wed, 2 Nov 2005 12:01:03 -0500 25, 24 -- Message-ID: {000e01c5dfcf$06f019b0$6501a8c0-at-Ken} 25, 24 -- MIME-Version: 1.0 25, 24 -- Content-Type: text/plain; 25, 24 -- charset="us-ascii" 25, 24 -- X-Priority: 3 (Normal) 25, 24 -- X-MSMail-Priority: Normal 25, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 25, 24 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 25, 24 -- Importance: Normal 25, 24 -- In-Reply-To: {200511021346.jA2DkcLd017977-at-ns.microscopy.com} 25, 24 -- X-IMSTrailer: __IMail_7__ 25, 24 -- Content-Transfer-Encoding: 8bit 25, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA2H1TZg001283 ==============================End of - Headers==============================
What are the options for increasing probe current? I.e., what things can you do to increase it? yes, increasing the aperture size will increase probe current. However, it increases probe diameter. Another option is to increase KV. This will greatly increase volumetric interacion such that more info comes from deeper in the specimen. It is a constant trade-off problem.
Before changing anything above, be sure your specimen is at the optimal analytical working distance (WD) for the EDS. If you don't have that value, you can find it by experimentation. Just start at a relatively long WD, check the counts per second and then start reducing WD. Keep checking the cps at each successive reduction in WD. The cps will start to increase and then decrease. The WD at max cps is your analytical WD. Work at this WD.
Now, back to aperture size and KV. I'd start with KV first. Based on the highest Z you are looking at, you will need about 2X the eV of the line value you are collecting. Look at the M, K and L series values and determine the necessary KV. E.g., Aluminum. Z=13. No L-series lines. Ka=1.486KeV. Kb=1.553KeV. No N-series. So, K-series are the only ones (BTW, Ka is the one used for calibration along with Cu Ka at 8.040KeV.). So for Al, 5KV would probably be a good value. If the specimen is bulk, then higher KV is OK and would increase probe current and cps.
Take an extreme such as W (Z=74). Here, we have lines at all shell series. However, not all of them are practical for normal SEM. Ka=59.305KeV (can't use this since KV would need to be about 120KV). Same problem with Kb. Ma=1.774KeV. Sorting through the other lines, La pops up at 8.394KeV. So 2X this would be 16KV. So, 18KV-20KV would be appropriate. But at this KV, volumetric interaction would be very high. High Z and high KV will result in skyrocketing cps. So, smaller aperture would be necessary to keep dead time down to {30% or so. If you want to quant, this is probably the appropriate condition. However, for mapping, if you do not have a volumetric interaction situation, and you do not need high resolution, you can increase KV and aperture size to increase cps. Keep in mind that most EDS systems need cps to be such that dead time is {=35% or thereabouts. Increasing cps with resulting higher DT simply throws away data since the pulse processor cannot handle the high number of counts. Each system has their own limit on this. Just look at DT to be sure the processor is not overloaded.
Again, if the specimen is bulk versus very small, then increasing aperture size will increase probe current and cps at the expense of resolution. But for low mag, bulk specimens, not much of an issue.
Hope this helps.
gary g.
At 05:45 AM 11/2/2005, you wrote:
} November 1, 2005 at 21:49:12 } Remember to consider the Grade/Age of the student when considering } the Question } --------------------------------------------------------------------------- } Please reply to both Willem.Wennekes-at-comcast.net as well as to } the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: Willem.Wennekes-at-comcast.net } Name: Willem Wennekes } } Organization: UES } } Education: Graduate College } } Location: Dayton, OH, USA } } Title: edx } } Question: Hi, What would be the benefit of using a larger } objective apperture instead of increasing the probe current in order } to increase the beam current for edx analysis and x-ray mapping? } } Cheers, } Willem } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 12 -- From zaluzec-at-ultra5.microscopy.com Wed Nov 2 07:44:18 2005 } 9, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } jA2DiH73012579 } 9, 12 -- for {microscopy-at-microscopy.com} ; Wed, 2 Nov 2005 } 07:44:17 -0600 } 9, 12 -- Mime-Version: 1.0 } 9, 12 -- X-Sender: zaluzec-at-ultra5.microscopy.com (Unverified) } 9, 12 -- Message-Id: {p06110406bf8e730200cf-at-[206.69.208.22]} } 9, 12 -- Date: Wed, 2 Nov 2005 07:44:17 -0600 } 9, 12 -- To: microscopy-at-microscopy.com } 9, 12 -- From: Willem.Wennekes-at-comcast.net (by way of Ask-A-Microscopist) } 9, 12 -- Subject: [Filtered] AskAMicroscopist: Aperture vs Current } for EDX analysis } 9, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
==============================Original Headers============================== 16, 25 -- From gary-at-gaugler.com Wed Nov 2 11:34:55 2005 16, 25 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 16, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jA2HYsAi010465 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 2 Nov 2005 11:34:54 -0600 16, 25 -- Received: (qmail 14508 invoked from network); 2 Nov 2005 09:34:38 -0800 16, 25 -- Received: by simscan 1.1.0 ppid: 14484, pid: 14485, t: 2.8882s 16, 25 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1151 spam: 3.0.3 16, 25 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 16, 25 -- by qsmtp3 with SMTP; 2 Nov 2005 09:34:35 -0800 16, 25 -- Message-Id: {6.2.3.4.2.20051102085421.02953898-at-mail.calweb.com} 16, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 16, 25 -- Date: Wed, 02 Nov 2005 09:34:51 -0800 16, 25 -- To: Willem.Wennekes-at-comcast.net 16, 25 -- From: Gary Gaugler {gary-at-gaugler.com} 16, 25 -- Subject: Re: [Microscopy] AskAMicroscopist: Aperture vs Current for EDX 16, 25 -- analysis 16, 25 -- Cc: MSA listserver {microscopy-at-microscopy.com} 16, 25 -- In-Reply-To: {200511021345.jA2Djs7P015905-at-ns.microscopy.com} 16, 25 -- References: {200511021345.jA2Djs7P015905-at-ns.microscopy.com} 16, 25 -- Mime-Version: 1.0 16, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 16, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp3.surewest.net 16, 25 -- X-Spam-Level: 16, 25 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 16, 25 -- version=3.0.3 ==============================End of - Headers==============================
=================================================== "Selecting a Digital Camera for Light Microscopy." ===================================================
Details are below.
Connection lines are limited, so reserve yours now. There is no charge to participate in this on-line seminar.
For Wednesday, 9-November, 10:00 AM (New York time), pre-register at: https://premconf.webex.com/premconf/j.php?ED=85888757&RG=1
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[ If any of the links have wrapped, please re-build it in your web browser's address bar. The line begins with "https" and ends with "RG=1" ]
Details: ============
"Selecting a Digital Camera for Light Microscopy." Presented by David Hitrys, QImaging Corporation
Attendee's will learn about digital cameras, how to interpret their performance specifications, and will leave with enhanced skills for critically analyzing a camera's suitability for various applications.
Outline of Topics:
-Understanding Digital Camera Parameters: -Camera Sensitivity -Noise Limitations -Dynamic Range (Full-Well Capacity) -Use of Gain -Color Acquisition Options -Note on Under- and Over-Sampling -Matching Pixel Size to Optical Resolution -Questions from the Audience
Presented by QImaging Corporation, makers of precision cameras for microscopy, the information imparted will be broadly useful to anyone striving to make the best camera choice for their imaging goals.
This seminar requires that attendees use a Java-enabled browser with a high bandwidth connection. Audio is via toll-free telephone.
There is no charge to participate in this on-line seminar.
==============================Original Headers============================== 16, 20 -- From dhitrys-at-qimaging.com Wed Nov 2 11:56:12 2005 16, 20 -- Received: from smtp01.mrf.mail.rcn.net (smtp01.mrf.mail.rcn.net [207.172.4.61]) 16, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA2HuC2i019371 16, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Nov 2005 11:56:12 -0600 16, 20 -- Received: from 209-6-80-225.c3-0.frm-ubr2.sbo-frm.ma.cable.rcn.com (HELO DHPC) ([209.6.80.225]) 16, 20 -- by smtp01.mrf.mail.rcn.net with ESMTP; 02 Nov 2005 12:56:09 -0500 16, 20 -- Message-Id: {4g7bqh$3dbd33-at-smtp01.mrf.mail.rcn.net} 16, 20 -- X-IronPort-AV: i="3.97,282,1125892800"; 16, 20 -- d="scan'208"; a="114668643:sNHT9149979018" 16, 20 -- From: "David Hitrys" {dhitrys-at-qimaging.com} 16, 20 -- To: {Microscopy-at-microscopy.com} 16, 20 -- Subject: Online Seminar: Selecting a Digital Camera for Light Microscopy 16, 20 -- Date: Wed, 2 Nov 2005 12:56:01 -0500 16, 20 -- MIME-Version: 1.0 16, 20 -- Content-Type: text/plain; 16, 20 -- charset="us-ascii" 16, 20 -- Content-Transfer-Encoding: 7bit 16, 20 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353 16, 20 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 16, 20 -- Thread-index: AcXf1q/Hi4L/7q/rSpS5zxm9fntrtw== ==============================End of - Headers==============================
Hi all, Has anyone used the new TEM support films from DuraSiN? I'd appreciate hearing your opinions on this product.
SINcerely, Beth
********************************************************************** Beth Richardson EM Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
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Is anyone aware of an independent company who offers yearly service contracts on LEO field emission SEMS. I would appreciate any info possible.
Thanks, Lori Ables laable-at-solutia.com
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==============================Original Headers============================== 5, 24 -- From laable-at-solutia.com Wed Nov 2 13:30:31 2005 5, 24 -- Received: from mailgw2.solutia.com (mailgw2.solutia.com [12.104.183.7]) 5, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA2JUV4R005955 5, 24 -- for {Microscopy-at-Microscopy.Com} ; Wed, 2 Nov 2005 13:30:31 -0600 5, 24 -- Received: from USAHMSLSOIEX2.soi.dir.solutia.com ([205.191.166.99]) by mailgw2.solutia.com with Microsoft SMTPSVC(6.0.3790.211); Wed, 2 Nov 2005 14:30:31 -0500 5, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.181 5, 24 -- Content-class: urn:content-classes:message 5, 24 -- MIME-Version: 1.0 5, 24 -- Content-Type: text/plain; 5, 24 -- charset="us-ascii" 5, 24 -- Subject: SEM - repair service 5, 24 -- Date: Wed, 2 Nov 2005 14:30:27 -0500 5, 24 -- Message-ID: {3412722F2EEF2B4F8658F0CA26C261E6023248-at-USAHMSLSOIEX2.soi.dir.solutia.com} 5, 24 -- Priority: normal 5, 24 -- Importance: normal 5, 24 -- X-MS-Has-Attach: 5, 24 -- X-MS-TNEF-Correlator: 5, 24 -- Thread-Topic: SEM - repair service 5, 24 -- Thread-Index: AcXf4+BwTx+PxmWgRMm9midBN4R0Uw== 5, 24 -- From: "Ables, Lori A" {laable-at-solutia.com} 5, 24 -- To: {Microscopy-at-Microscopy.Com} 5, 24 -- X-OriginalArrivalTime: 02 Nov 2005 19:30:31.0256 (UTC) FILETIME=[E3377580:01C5DFE3] 5, 24 -- Content-Transfer-Encoding: 8bit 5, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA2JUV4R005955 ==============================End of - Headers==============================
The statement that "increasing the aperture size ... increases probe diameter" is not always true. The relationship between beam diameter and beam current can be found in many references (e.g., equation 2.14 of the 2nd edition of Goldstein). What this equation shows is that all four of the terms that control beam diameter vary as the beam half-angle (alpha) which is proportional to final aperture diameter. However, two of the terms involve alpha in the numerator, and two in the denominator. Thus, for any given probe current (Ip) there is an optimum aperture size that gives the minimum beam diameter. Either a smaller or larger aperture than called for by the minimum condition will make the beam diameter larger.
Thus one very good reason for sometimes using a larger aperture size to increase beam current rather than just cranking up the Spot control (less demagnification) is to better match the condition for minimum spot size .
Fred Schamber ASPEX Corp.
gary-at-gaugler.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } What are the options for increasing probe current? I.e., what } things can you do to increase it? yes, increasing the aperture } size will increase probe current. However, it increases probe } diameter. Another option is to increase KV. This will greatly } increase volumetric interacion such that more info comes from } deeper in the specimen. It is a constant trade-off problem. } } Before changing anything above, be sure your specimen is at } the optimal analytical working distance (WD) for the EDS. } If you don't have that value, you can find it by experimentation. } Just start at a relatively long WD, check the counts per second } and then start reducing WD. Keep checking the cps at each } successive reduction in WD. The cps will start to increase and } then decrease. The WD at max cps is your analytical WD. Work } at this WD. } } Now, back to aperture size and KV. I'd start with KV first. } Based on the highest Z you are looking at, you will need about } 2X the eV of the line value you are collecting. Look at the M, K } and L series values and determine the necessary KV. E.g., Aluminum. } Z=13. No L-series lines. Ka=1.486KeV. Kb=1.553KeV. No N-series. } So, K-series are the only ones (BTW, Ka is the one used for calibration } along with Cu Ka at 8.040KeV.). So for Al, 5KV would probably } be a good value. If the specimen is bulk, then higher KV is OK } and would increase probe current and cps. } } Take an extreme such as W (Z=74). Here, we have lines at all } shell series. However, not all of them are practical for normal } SEM. Ka=59.305KeV (can't use this since KV would need to be about } 120KV). Same problem with Kb. Ma=1.774KeV. Sorting through the } other lines, La pops up at 8.394KeV. So 2X this would be 16KV. } So, 18KV-20KV would be appropriate. But at this KV, volumetric } interaction would be very high. High Z and high KV will result } in skyrocketing cps. So, smaller aperture would be necessary } to keep dead time down to {30% or so. If you want to quant, } this is probably the appropriate condition. However, for mapping, } if you do not have a volumetric interaction situation, and you } do not need high resolution, you can increase KV and aperture } size to increase cps. Keep in mind that most EDS systems need } cps to be such that dead time is {=35% or thereabouts. Increasing } cps with resulting higher DT simply throws away data since the } pulse processor cannot handle the high number of counts. Each } system has their own limit on this. Just look at DT to be sure } the processor is not overloaded. } } Again, if the specimen is bulk versus very small, then increasing } aperture size will increase probe current and cps at the expense of } resolution. But for low mag, bulk specimens, not much of an issue. } } Hope this helps. } } gary g. } } } } } } } } At 05:45 AM 11/2/2005, you wrote: } } } } November 1, 2005 at 21:49:12 } } Remember to consider the Grade/Age of the student when considering } } the Question } } --------------------------------------------------------------------------- } } Please reply to both Willem.Wennekes-at-comcast.net as well as to } } the Microscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: Willem.Wennekes-at-comcast.net } } Name: Willem Wennekes } } } } Organization: UES } } } } Education: Graduate College } } } } Location: Dayton, OH, USA } } } } Title: edx } } } } Question: Hi, What would be the benefit of using a larger } } objective apperture instead of increasing the probe current in order } } to increase the beam current for edx analysis and x-ray mapping? } } } } Cheers, } } Willem } } } } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } } 9, 12 -- From zaluzec-at-ultra5.microscopy.com Wed Nov 2 07:44:18 2005 } } 9, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } } 9, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } } jA2DiH73012579 } } 9, 12 -- for {microscopy-at-microscopy.com} ; Wed, 2 Nov 2005 } } 07:44:17 -0600 } } 9, 12 -- Mime-Version: 1.0 } } 9, 12 -- X-Sender: zaluzec-at-ultra5.microscopy.com (Unverified) } } 9, 12 -- Message-Id: {p06110406bf8e730200cf-at-[206.69.208.22]} } } 9, 12 -- Date: Wed, 2 Nov 2005 07:44:17 -0600 } } 9, 12 -- To: microscopy-at-microscopy.com } } 9, 12 -- From: Willem.Wennekes-at-comcast.net (by way of Ask-A-Microscopist) } } 9, 12 -- Subject: [Filtered] AskAMicroscopist: Aperture vs Current } } for EDX analysis } } 9, 12 -- Content-Type: text/plain; charset="us-ascii" } } ==============================End of - Headers============================== } } } } ==============================Original Headers============================== } 16, 25 -- From gary-at-gaugler.com Wed Nov 2 11:34:55 2005 } 16, 25 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) } 16, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jA2HYsAi010465 } 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 2 Nov 2005 11:34:54 -0600 } 16, 25 -- Received: (qmail 14508 invoked from network); 2 Nov 2005 09:34:38 -0800 } 16, 25 -- Received: by simscan 1.1.0 ppid: 14484, pid: 14485, t: 2.8882s } 16, 25 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1151 spam: 3.0.3 } 16, 25 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) } 16, 25 -- by qsmtp3 with SMTP; 2 Nov 2005 09:34:35 -0800 } 16, 25 -- Message-Id: {6.2.3.4.2.20051102085421.02953898-at-mail.calweb.com} } 16, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 } 16, 25 -- Date: Wed, 02 Nov 2005 09:34:51 -0800 } 16, 25 -- To: Willem.Wennekes-at-comcast.net } 16, 25 -- From: Gary Gaugler {gary-at-gaugler.com} } 16, 25 -- Subject: Re: [Microscopy] AskAMicroscopist: Aperture vs Current for EDX } 16, 25 -- analysis } 16, 25 -- Cc: MSA listserver {microscopy-at-microscopy.com} } 16, 25 -- In-Reply-To: {200511021345.jA2Djs7P015905-at-ns.microscopy.com} } 16, 25 -- References: {200511021345.jA2Djs7P015905-at-ns.microscopy.com} } 16, 25 -- Mime-Version: 1.0 } 16, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } 16, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp3.surewest.net } 16, 25 -- X-Spam-Level: } 16, 25 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham } 16, 25 -- version=3.0.3 } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 25 -- From schamber-at-aspexllc.com Wed Nov 2 13:54:04 2005 4, 25 -- Received: from mail26.atl.registeredsite.com (mail26.atl.registeredsite.com [216.247.37.21]) 4, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA2Js4PV015105 4, 25 -- for {microscopy-at-microscopy.com} ; Wed, 2 Nov 2005 13:54:04 -0600 4, 25 -- Received: from imta02a2.registeredsite.com (imta02a2.registeredsite.com [64.225.255.11]) 4, 25 -- by mail26.atl.registeredsite.com (8.12.11/8.12.11) with ESMTP id jA2Js1GA000430; 4, 25 -- Wed, 2 Nov 2005 14:54:01 -0500 4, 25 -- Received: from [192.168.1.89] ([67.141.199.81]) 4, 25 -- by imta02a2.registeredsite.com with ESMTP 4, 25 -- id {20051102195400.MIBK5784.imta02a2.registeredsite.com-at-[192.168.1.89]} ; 4, 25 -- Wed, 2 Nov 2005 14:54:00 -0500 4, 25 -- Message-ID: {43691958.6030608-at-aspexllc.com} 4, 25 -- Date: Wed, 02 Nov 2005 14:54:00 -0500 4, 25 -- From: Frederick Schamber {schamber-at-aspexllc.com} 4, 25 -- Reply-To: schamber-at-aspexllc.com 4, 25 -- Organization: Aspex Instruments 4, 25 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 4, 25 -- X-Accept-Language: en-us, en 4, 25 -- MIME-Version: 1.0 4, 25 -- To: gary-at-gaugler.com, microscopy-at-microscopy.com 4, 25 -- Subject: Re: [Microscopy] Re: AskAMicroscopist: Aperture vs Current for EDX 4, 25 -- References: {200511021737.jA2HbgKP015764-at-ns.microscopy.com} 4, 25 -- In-Reply-To: {200511021737.jA2HbgKP015764-at-ns.microscopy.com} 4, 25 -- Content-Type: text/plain; charset=us-ascii; format=flowed 4, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both CGoldsmith-at-cdc.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: Centers for Disease Control and Prevention (CDC)
Title-Subject: [Filtered] MListserver:Used FEI EM410-LS is available
Question: The Centers for Disease Control and Prevention (CDC) has a used FEI EM410-LS that is available for transfer to a government facility (federal, state, or local) or an educational institution (university, college, high school, etc.). Microscope is 21 years old, and in good condition. Please contact me off-line at CGoldsmith-at-cdc.gov for more information.
Cynthia S. Goldsmith, M.S. Infectious Disease Pathology Activity Centers for Disease Control and Prevention (CDC) Phone: (404)639-3306 Fax: (404)639-1377
This follows published info. However, the question was about aperture size. The other variable of condenser squeeze or relaxation is something else to throw into the pot. We don't know if his system has a condenser control. Probably so.
He was looking for increase in probe current. I figure that it is safe to say that everything else being equal, increasing the aperture size will increase probe current. That is really all he wants to do/know--notwithstanding all the other nuances. He just wants to know about a larger final aperture size.
gary g.
At 11:55 AM 11/2/2005, you wrote:
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==============================Original Headers============================== 10, 24 -- From gary-at-gaugler.com Wed Nov 2 18:16:53 2005 10, 24 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jA30GrMB003901 10, 24 -- for {microscopy-at-microscopy.com} ; Wed, 2 Nov 2005 18:16:53 -0600 10, 24 -- Received: (qmail 8475 invoked from network); 2 Nov 2005 16:16:36 -0800 10, 24 -- Received: by simscan 1.1.0 ppid: 8456, pid: 8458, t: 4.8519s 10, 24 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1160 spam: 3.0.3 10, 24 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 24 -- by qsmtp3 with SMTP; 2 Nov 2005 16:16:31 -0800 10, 24 -- Message-Id: {6.2.3.4.2.20051102160810.02947458-at-mail.calweb.com} 10, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 10, 24 -- Date: Wed, 02 Nov 2005 16:16:48 -0800 10, 24 -- To: schamber-at-aspexllc.com 10, 24 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 24 -- Subject: Re: [Microscopy] AskAMicroscopist: Aperture vs Current for EDX 10, 24 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 24 -- In-Reply-To: {200511021955.jA2JtlmF018421-at-ns.microscopy.com} 10, 24 -- References: {200511021955.jA2JtlmF018421-at-ns.microscopy.com} 10, 24 -- Mime-Version: 1.0 10, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 10, 24 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp3.surewest.net 10, 24 -- X-Spam-Level: 10, 24 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 10, 24 -- version=3.0.3 ==============================End of - Headers==============================
Hi again, I've been asked to rephrase my question about silicon nitride membrane window grids. If you use either the SPI silicon nitride membrane window grids or the EMS DuraSiN silicon nitride membrane window grids I'd like to hear your opinion of them. Thanks, Beth
********************************************************************** Beth Richardson EM Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
I think there are two issues here. One is the "physics" question (what does theory say? ) and the other a practical question (what happens in an actual instrument operating in a normal way?).
First the physics. Here the ultimate limit is diffraction. Regardless of the source type, a too-small aperture will cause a diffraction spreading of the beam. The simplest way to understand this is as a manifestation of the Heisenberg uncertainty principle -- when the lateral location of the electron is too tightly constrained by the aperture, then the lateral uncertainty in the momentum increases and there is broadening. So if one were operating a SEM in a difraction-limited mode, increasing the aperture size would be the most sensible way to increase the beam current.
Most thermionic (not FE) SEMs are not operated anywhere near the diffraction limit. Usually the dominant limit to resolution is spherical aberration and that term varies as aperture size to the third power. The simple quadrature approximation shown as equation 2.14 in Goldstein (2nd Ed) is generally regarded as valid for thermionic SEMs, but is not valid for any instrument operating near the diffraction limit. Here one must use much more complex wave-function calculations -- it's been a long time since I did this, but the role of the aperture is the same. There is an optimum aperture size for any given beam current and either a smaller or larger aperture than that optimum will increase the beam diameter.
Now for the practical. What actually happens when you turn a knob or change an aperture on a particular instrument depends on the details of how that instrument was designed and is being operated. When a SEM employs a "virtual" aperture (the physical aperture doesn't reside in the principal plane of the probe-forming lens) then it is possible to change the *effective* aperture size by manipulating the condenser lens(es) and thus the effective aperture size can be optimized by the control software to be optimal for that probe current without changing the physical aperture. Even if that is not the case, it's usually impractical or inconvenient to optimally match the physical aperture to its ideal size (apertures tend to be available in rather coarse diameter steps). Consequently, for modest changes in beam current, just turning the "Spot" control (or whatever you call the control that regulates the condenser current) is the simple practical expedient. But in more extreme cases, where one wants to drastically increase the beam current but doesn't want to unduly enlarge the spot, one is well advised to pay attention to the physics -- the principle works.
Now, back to the original question that was asked. "What would be the benefit of using a larger objective apperture instead of increasing the probe current in order to increase the beam current for edx analysis and x-ray mapping?" There are certainly many situations where choosing a larger aperture size will permit the higher probe current required for x-ray analysis to be achieved with minimal degradation of beam diameter. That is a practical reality in many situations. There may be other good reasons, and this answer won't apply to all situations. But it is a mechanism that needs to be considered given the limited information provided. So far from being a "nuance," depending on the instrument being referenced, this may in fact be the most pertinent answer to the question posed.
Fred Schamber ASPEX Corp.
gary-at-gaugler.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This follows published info. However, the question was } about aperture size. The other variable of condenser } squeeze or relaxation is something else to throw into the } pot. We don't know if his system has a condenser control. } Probably so. } } He was looking for increase in probe current. I figure } that it is safe to say that everything else being equal, } increasing the aperture size will increase probe current. } That is really all he wants to do/know--notwithstanding all } the other nuances. He just wants to know about a larger } final aperture size. } } gary g. } } } } At 11:55 AM 11/2/2005, you wrote: } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 7, 25 -- From schamber-at-aspexllc.com Thu Nov 3 11:16:45 2005 7, 25 -- Received: from mail24.atl.registeredsite.com (mail24.atl.registeredsite.com [216.247.37.44]) 7, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA3HGjGh002788 7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 3 Nov 2005 11:16:45 -0600 7, 25 -- Received: from imta06a2.registeredsite.com (imta06a2.registeredsite.com [64.225.255.15]) 7, 25 -- by mail24.atl.registeredsite.com (8.12.11/8.12.11) with ESMTP id jA3HGeQm027337; 7, 25 -- Thu, 3 Nov 2005 12:16:40 -0500 7, 25 -- Received: from [192.168.1.89] ([67.141.199.81]) 7, 25 -- by imta06a2.registeredsite.com with ESMTP 7, 25 -- id {20051103171640.BAZB13088.imta06a2.registeredsite.com-at-[192.168.1.89]} ; 7, 25 -- Thu, 3 Nov 2005 12:16:40 -0500 7, 25 -- Message-ID: {436A45F7.6050904-at-aspexllc.com} 7, 25 -- Date: Thu, 03 Nov 2005 12:16:39 -0500 7, 25 -- From: Frederick Schamber {schamber-at-aspexllc.com} 7, 25 -- Reply-To: schamber-at-aspexllc.com 7, 25 -- Organization: Aspex Instruments 7, 25 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 7, 25 -- X-Accept-Language: en-us, en 7, 25 -- MIME-Version: 1.0 7, 25 -- To: gary-at-gaugler.com, microscopy-at-microscopy.com 7, 25 -- Subject: Re: [Microscopy] Re: AskAMicroscopist: Aperture vs Current for EDX 7, 25 -- References: {200511030020.jA30K3PI009216-at-ns.microscopy.com} 7, 25 -- In-Reply-To: {200511030020.jA30K3PI009216-at-ns.microscopy.com} 7, 25 -- Content-Type: text/plain; charset=us-ascii; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Lori, I've had a number of requests in the past year or so to service LEO equipment. The problem is that they don't provide schematics and a third party can't come in and have any hope of being competent without them.
As far as I know, every other manufacturer provides schematics with their system.
Perhaps someone from LEO would like to comment.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: laable-at-solutia.com [mailto:laable-at-solutia.com] Sent: Wednesday, November 02, 2005 2:34 PM To: kenconverse-at-qualityimages.biz
Is anyone aware of an independent company who offers yearly service contracts on LEO field emission SEMS. I would appreciate any info possible.
Thanks, Lori Ables laable-at-solutia.com
This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain Solutia confidential and privileged information. The recipient is hereby put on notice to treat the information as confidential and privileged and to not disclose or use the information except as authorized by Solutia. Any unauthorized review, printing, retention, copying, disclosure, distribution, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you received this message in error, please immediately contact the sender by reply email and delete all copies of the material from any computer. Thank you for your cooperation.
==============================Original Headers============================== 5, 24 -- From laable-at-solutia.com Wed Nov 2 13:30:31 2005 5, 24 -- Received: from mailgw2.solutia.com (mailgw2.solutia.com [12.104.183.7]) 5, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA2JUV4R005955 5, 24 -- for {Microscopy-at-Microscopy.Com} ; Wed, 2 Nov 2005 13:30:31 -0600 5, 24 -- Received: from USAHMSLSOIEX2.soi.dir.solutia.com ([205.191.166.99]) by mailgw2.solutia.com with Microsoft SMTPSVC(6.0.3790.211); Wed, 2 Nov 2005 14:30:31 -0500 5, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.181 5, 24 -- Content-class: urn:content-classes:message 5, 24 -- MIME-Version: 1.0 5, 24 -- Content-Type: text/plain; 5, 24 -- charset="us-ascii" 5, 24 -- Subject: SEM - repair service 5, 24 -- Date: Wed, 2 Nov 2005 14:30:27 -0500 5, 24 -- Message-ID: {3412722F2EEF2B4F8658F0CA26C261E6023248-at-USAHMSLSOIEX2.soi.dir.solutia.com} 5, 24 -- Priority: normal 5, 24 -- Importance: normal 5, 24 -- X-MS-Has-Attach: 5, 24 -- X-MS-TNEF-Correlator: 5, 24 -- Thread-Topic: SEM - repair service 5, 24 -- Thread-Index: AcXf4+BwTx+PxmWgRMm9midBN4R0Uw== 5, 24 -- From: "Ables, Lori A" {laable-at-solutia.com} 5, 24 -- To: {Microscopy-at-Microscopy.Com} 5, 24 -- X-OriginalArrivalTime: 02 Nov 2005 19:30:31.0256 (UTC) FILETIME=[E3377580:01C5DFE3] 5, 24 -- Content-Transfer-Encoding: 8bit 5, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA2JUV4R005955 ==============================End of - Headers==============================
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==============================Original Headers============================== 23, 23 -- From kenconverse-at-qualityimages.biz Thu Nov 3 14:18:35 2005 23, 23 -- Received: from qualityimages.biz (dpmail16.doteasy.com [65.61.209.16]) 23, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA3KIY2E014009 23, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 3 Nov 2005 14:18:35 -0600 23, 23 -- Received: from Ken [68.64.242.101] by qualityimages.biz with ESMTP 23, 23 -- (SMTPD32-8.05) id A0222B8200F8; Thu, 03 Nov 2005 12:16:34 -0800 23, 23 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 23, 23 -- To: {laable-at-solutia.com} , "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 23, 23 -- Subject: RE: [Microscopy] SEM - repair service 23, 23 -- Date: Thu, 3 Nov 2005 15:18:25 -0500 23, 23 -- Message-ID: {004001c5e0b3$c371cc70$6501a8c0-at-Ken} 23, 23 -- MIME-Version: 1.0 23, 23 -- Content-Type: text/plain; 23, 23 -- charset="us-ascii" 23, 23 -- X-Priority: 3 (Normal) 23, 23 -- X-MSMail-Priority: Normal 23, 23 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 23, 23 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 23, 23 -- Importance: Normal 23, 23 -- In-Reply-To: {200511021933.jA2JXlJu011438-at-ns.microscopy.com} 23, 23 -- X-IMSTrailer: __IMail_7__ 23, 23 -- Content-Transfer-Encoding: 8bit 23, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA3KIY2E014009 ==============================End of - Headers==============================
I am writing to see if there is anyone has the following book: ****** Thin film technology handbook By: Aicha A. R. Elshabini-Riad, Fred D. Barlow III. c1998 ******
It is not available now at the university library and all what I need is to read pages 27:34, so I hope if there is someone has it and scan those pages and send them to me. I really need them urgently.
Thanks in advance and I appreciate your time and help.
Best Regards
Hany
-- ********************************************************** Hany Ramadan Graduate student Chemistry department McMaster university, Hamilton, Ontario, Canada 905-525-9140 x: 26322 elsayeh-at-mcmaster.ca **********************************************************
==============================Original Headers============================== 9, 23 -- From ramadanhany-at-gmail.com Thu Nov 3 20:10:34 2005 9, 23 -- Received: from xproxy.gmail.com (xproxy.gmail.com [66.249.82.206]) 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA42AXr0028181 9, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Nov 2005 20:10:34 -0600 9, 23 -- Received: by xproxy.gmail.com with SMTP id s19so723696wxc 9, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 03 Nov 2005 18:10:33 -0800 (PST) 9, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 23 -- s=beta; d=gmail.com; 9, 23 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 9, 23 -- b=QqhWozLS8k5S5x9bjQM775PxfJMfA9LlxgVlo4WjOnodhSCdsQPMy6tn1kdeqr2YQcgYVZy1cfZs47bOBfGcXFDuKuVXFLuyHcyDIecQ27lUANDbFfxaKyBY2jdWm/XzD6EIQccBUDEkJevk5PTsbbv11kB0M+O+ntc1yhe/IUU= 9, 23 -- Received: by 10.65.240.7 with SMTP id s7mr1542724qbr; 9, 23 -- Thu, 03 Nov 2005 18:10:33 -0800 (PST) 9, 23 -- Received: by 10.65.158.13 with HTTP; Thu, 3 Nov 2005 18:10:33 -0800 (PST) 9, 23 -- Message-ID: {8d8ce5a30511031810k5b3061bg7542a341bf2c5c9b-at-mail.gmail.com} 9, 23 -- Date: Thu, 3 Nov 2005 21:10:33 -0500 9, 23 -- From: Hany Ramadan {ramadanhany-at-gmail.com} 9, 23 -- To: Microscopy-at-microscopy.com 9, 23 -- Subject: A favor needed....Thin film technology handbook.... 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; charset=ISO-8859-1 9, 23 -- Content-Disposition: inline 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA42AXr0028181 ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (akoorts-at-medic.up.ac.za) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, November 4, 2005 at 01:39:39 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both akoorts-at-medic.up.ac.za as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: I loose a lot of sections from uncoated 200 mesh nickel grids during immunolocalization (including autoclave antigen retrieval) and in situ hybridization (including autoclave pre-treatment). Suggestions to appropriate support films that would not interfere with the immunolocalization and in situ hybridization reactions?
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Email: marta.taules-at-uab.es Name: Marta Taules
Organization: UAB
Title-Subject: [Filtered] MListserver:
Question: Dear collegues,
I would like to know which should be the purity of ethane in order to apply the immersion cryo fixation with forceps injector (CPC equipment from Leica).
Thanks Jim for your email, here are the details: This book is available at the library but it is checked out and it may take more than 10 days to recall it and I need it for a report that is due in a few days. I can not use ILL to get it because the library already has it but it is just checked out.
Thanks
Best Regards
Hany
On 11/4/05, Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} wrote: } Hany } } Your library has a ILL (Inter Library Loan) department } that will do that for you. } } regards, } } Jim } } } From mail-at-ns.microscopy.com Thu Nov 3 21:14:19 2005 } } Return-Path: {mail-at-ns.microscopy.com} } } Received: from ns.microscopy.com (microscopy.com [206.69.208.10]) } } by www.matscieng.sunysb.edu (8.11.6/8.11.6) with ESMTP id jA42EIT23497 } } for {jquinn-at-www.matscieng.sunysb.edu} ; Thu, 3 Nov 2005 21:14:18 -0500 } } Received: from ns.microscopy.com (localhost.localdomain [127.0.0.1]) } } by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA42CBIi029593 } } for {jquinn-at-www.matscieng.sunysb.edu} ; Thu, 3 Nov 2005 20:12:11 -0600 } } Received: (from mail-at-localhost) } } by ns.microscopy.com (8.12.11/8.12.10/Submit) id jA42CBDk029591; } } Thu, 3 Nov 2005 20:12:11 -0600 } } Date: Thu, 3 Nov 2005 20:12:11 -0600 } } Message-Id: {200511040212.jA42CBDk029591-at-ns.microscopy.com} } } To: jquinn-at-www.matscieng.sunysb.edu } } From: ramadanhany-at-gmail.com } } Reply-to: ramadanhany-at-gmail.com } } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } } Subject: [Microscopy] A favor needed....Thin film technology handbook.... } } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } } X-lewp: MicroscopyListSpam NAGS } } Status: R } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi guys, } } } } I am writing to see if there is anyone has the following book: } } ****** } } Thin film technology handbook } } By: Aicha A. R. Elshabini-Riad, Fred D. Barlow III. } } c1998 } } ****** } } } } It is not available now at the university library and all what I need } } is to read pages 27:34, so I hope if there is someone has it and scan } } those pages and send them to me. I really need them urgently. } } } } } } Thanks in advance and I appreciate your time and help. } } } } Best Regards } } } } Hany } } } } -- } } ********************************************************** } } Hany Ramadan } } Graduate student } } Chemistry department } } McMaster university, Hamilton, Ontario, Canada } } 905-525-9140 x: 26322 } } elsayeh-at-mcmaster.ca } } ********************************************************** } } } } } } ==============================Original Headers============================== } } 9, 23 -- From ramadanhany-at-gmail.com Thu Nov 3 20:10:34 2005 } } 9, 23 -- Received: from xproxy.gmail.com (xproxy.gmail.com [66.249.82.206]) } } 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA42AXr0028181 } } 9, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Nov 2005 20:10:34 -0600 } } 9, 23 -- Received: by xproxy.gmail.com with SMTP id s19so723696wxc } } 9, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 03 Nov 2005 18:10:33 -0800 (PST) } } 9, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } } 9, 23 -- s=beta; d=gmail.com; } } 9, 23 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } } 9, 23 -- b=QqhWozLS8k5S5x9bjQM775PxfJMfA9LlxgVlo4WjOnodhSCdsQPMy6tn1kdeqr2YQcgYVZy1cfZs47bOBfGcXFDuKuVXFLuyHcyDIecQ27lUANDbFfxaKyBY2jdWm/XzD6EIQccBUDEkJevk5PTsbbv11kB0M+O+ntc1yhe/IUU= } } 9, 23 -- Received: by 10.65.240.7 with SMTP id s7mr1542724qbr; } } 9, 23 -- Thu, 03 Nov 2005 18:10:33 -0800 (PST) } } 9, 23 -- Received: by 10.65.158.13 with HTTP; Thu, 3 Nov 2005 18:10:33 -0800 (PST) } } 9, 23 -- Message-ID: {8d8ce5a30511031810k5b3061bg7542a341bf2c5c9b-at-mail.gmail.com} } } 9, 23 -- Date: Thu, 3 Nov 2005 21:10:33 -0500 } } 9, 23 -- From: Hany Ramadan {ramadanhany-at-gmail.com} } } 9, 23 -- To: Microscopy-at-microscopy.com } } 9, 23 -- Subject: A favor needed....Thin film technology handbook.... } } 9, 23 -- MIME-Version: 1.0 } } 9, 23 -- Content-Type: text/plain; charset=ISO-8859-1 } } 9, 23 -- Content-Disposition: inline } } 9, 23 -- Content-Transfer-Encoding: 8bit } } 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA42AXr0028181 } } ==============================End of - Headers============================== } } }
-- ********************************************************** Hany Ramadan Graduate student Chemistry department McMaster university, Hamilton, Ontario, Canada 905-525-9140 x: 26322 elsayeh-at-mcmaster.ca **********************************************************
==============================Original Headers============================== 9, 25 -- From ramadanhany-at-gmail.com Fri Nov 4 12:28:42 2005 9, 25 -- Received: from wproxy.gmail.com (wproxy.gmail.com [64.233.184.202]) 9, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA4ISgCn001231 9, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Nov 2005 12:28:42 -0600 9, 25 -- Received: by wproxy.gmail.com with SMTP id i28so444210wra 9, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 04 Nov 2005 10:28:34 -0800 (PST) 9, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 25 -- s=beta; d=gmail.com; 9, 25 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 9, 25 -- b=l1juCdmmRL/43TDQAFPbVmKv2g+Rwd3QrwzCftMYS/A9OQiBE/E3V7Bl+SbOdWVAAA+uGvrCryrD2KpPLv1+9QaMsiaejNSNyWFHarWiBcfopNsjc1+pyOjx2giNJs613UYdgHHJwOkzqVYnoiNvEr1wFXYbHZdX1oGkPERhSD8= 9, 25 -- Received: by 10.65.93.3 with SMTP id v3mr2428896qbl; 9, 25 -- Fri, 04 Nov 2005 10:22:28 -0800 (PST) 9, 25 -- Received: by 10.65.158.7 with HTTP; Fri, 4 Nov 2005 10:22:28 -0800 (PST) 9, 25 -- Message-ID: {8d8ce5a30511041022y1f443c16o400d322d55d2bc2e-at-mail.gmail.com} 9, 25 -- Date: Fri, 4 Nov 2005 13:22:28 -0500 9, 25 -- From: Hany Ramadan {ramadanhany-at-gmail.com} 9, 25 -- To: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} , Microscopy-at-microscopy.com 9, 25 -- Subject: Re: [Microscopy] A favor needed....Thin film technology handbook.... 9, 25 -- In-Reply-To: {200511041600.jA4G0wq25043-at-www.matscieng.sunysb.edu} 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Type: text/plain; charset=ISO-8859-1 9, 25 -- Content-Disposition: inline 9, 25 -- References: {200511041600.jA4G0wq25043-at-www.matscieng.sunysb.edu} 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA4ISgCn001231 ==============================End of - Headers==============================
Maya Research Program is a non-profit corporation that supports archaeological research and is affiliated with Texas Christin University. We have been given a Zeiss Model 109 SEM. It was taken out of service by the University of Houston, but apparently is in very good condition.
We wish to sell this instrument. Does anyone know of someone who deals in such equipment?
Many thanks in advance.
Tom Guderjan
__________________________________ Yahoo! Mail - PC Magazine Editors' Choice 2005 http://mail.yahoo.com
==============================Original Headers============================== 8, 18 -- From guderjan-at-yahoo.com Fri Nov 4 15:51:46 2005 8, 18 -- Received: from web33015.mail.mud.yahoo.com (web33015.mail.mud.yahoo.com [68.142.206.79]) 8, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jA4Lpklm012524 8, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 4 Nov 2005 15:51:46 -0600 8, 18 -- Received: (qmail 37074 invoked by uid 60001); 4 Nov 2005 21:51:37 -0000 8, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 8, 18 -- s=s1024; d=yahoo.com; 8, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 8, 18 -- b=W4vfmpEUSHTKXsQk2cHg3WOnn94nQ+EjvQB+mMiv2QDmR48WtwCUM72sZL+5KUomgXHl/bQxMxuBXm6liwTTRj23U3Ioh+X+zUDq4XFm9RR3CesgVpKjiv6Vqbk0dpxhj/8Ag5fhidjvnpAqt09cGOBhOBiJqLQTUHGV0M7Pne4= ; 8, 18 -- Message-ID: {20051104215137.37072.qmail-at-web33015.mail.mud.yahoo.com} 8, 18 -- Received: from [65.67.114.117] by web33015.mail.mud.yahoo.com via HTTP; Fri, 04 Nov 2005 13:51:37 PST 8, 18 -- Date: Fri, 4 Nov 2005 13:51:37 -0800 (PST) 8, 18 -- From: thomas guderjan {guderjan-at-yahoo.com} 8, 18 -- Subject: Zeiss Model 109 8, 18 -- To: Microscopy-at-Microscopy.Com 8, 18 -- MIME-Version: 1.0 8, 18 -- Content-Type: text/plain; charset=iso-8859-1 8, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
A quick technical question - we are trying to do freeze substitution using 2%Osmium in acetone, but it goes black as we warm from -90 to -60. Have you done this and if so is this normal or is there something wrong with our Os/acetone? Acetone has been kept over a molecular sieve in dialysis tubing, I have read somewhere that molecular sieves should be avoided, could this be the problem? Acetone is at -20c before adding to osmium and then immediately frozen.
A.C.Richardson Experimental Officer School of Biological and Biomedical Science Centre for Molecular Imaging University of Durham Science site South Rd Durham England E-mail: a.c.richardson-at-durham.ac.uk
==============================Original Headers============================== 7, 17 -- From ac.richardson2-at-btinternet.com Sat Nov 5 12:32:35 2005 7, 17 -- Received: from smtp813.mail.ukl.yahoo.com (smtp813.mail.ukl.yahoo.com [217.12.12.203]) 7, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jA5IWYIS002827 7, 17 -- for {Microscopy-at-microscopy.com} ; Sat, 5 Nov 2005 12:32:35 -0600 7, 17 -- Received: (qmail 79103 invoked from network); 5 Nov 2005 18:32:34 -0000 7, 17 -- Received: from unknown (HELO ?192.168.1.4?) (ac.richardson2-at-btinternet.com-at-86.133.65.16 with plain) 7, 17 -- by smtp813.mail.ukl.yahoo.com with SMTP; 5 Nov 2005 18:32:34 -0000 7, 17 -- Message-ID: {436CFAC0.2030904-at-btinternet.com} 7, 17 -- Date: Sat, 05 Nov 2005 18:32:32 +0000 7, 17 -- From: Christine Richardson {ac.richardson2-at-btinternet.com} 7, 17 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 7, 17 -- X-Accept-Language: en-us, en 7, 17 -- MIME-Version: 1.0 7, 17 -- To: Microscopy-at-microscopy.com 7, 17 -- Subject: TEM: freeze substitution 7, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We have a project coming up for preparation of mosquito eyes for both LM and TEM examination. I was planning on using a glut-PAF fix in cacodylate buffer followed by osmium tetroxide, ETOH dehydration and embedding in epoxy generic resin.
Options are incubation with tannic acid and en bloc staining with UA prior to dehydration.
I would appreciate some suggestions as to preparation protocols. Have any of you tried the tannic acid incubation? If so, at what concentration and when...in both glut/PAF and Os or alone after glut/PAF? Have you used it at RT or 4oC? Has en bloc UA been helpful in increasing contrast in eye tissue?
Any suggestions would be most welcome.
Thanks, Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 7, 21 -- From dsherman-at-purdue.edu Sat Nov 5 15:54:57 2005 7, 21 -- Received: from exchange.purdue.edu (1061exfe03.adpc.purdue.edu [128.210.63.225]) 7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA5Lsu8i013417 7, 21 -- for {microscopy-at-microscopy.com} ; Sat, 5 Nov 2005 15:54:57 -0600 7, 21 -- Received: from EXCH02.purdue.lcl ([128.210.63.235]) by exchange.purdue.edu with Microsoft SMTPSVC(6.0.3790.211); 7, 21 -- Sat, 5 Nov 2005 16:54:51 -0500 7, 21 -- Received: from 12.222.30.139 ([12.222.30.139]) by EXCH02.purdue.lcl ([128.210.63.237]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.239]) with Microsoft Exchange Server HTTP-DAV ; 7, 21 -- Sat, 5 Nov 2005 21:54:51 +0000 7, 21 -- User-Agent: Microsoft-Entourage/11.2.0.050811 7, 21 -- Date: Sat, 05 Nov 2005 16:54:55 -0500 7, 21 -- Subject: Fixing insect eyes 7, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 7, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 7, 21 -- Message-ID: {BF92945F.6595%dsherman-at-purdue.edu} 7, 21 -- Thread-Topic: Fixing insect eyes 7, 21 -- Thread-Index: AcXiU45zzS6WK05GEdqCyQAKlcoUxg== 7, 21 -- Mime-version: 1.0 7, 21 -- Content-type: text/plain; 7, 21 -- charset="US-ASCII" 7, 21 -- Content-transfer-encoding: 7bit 7, 21 -- X-OriginalArrivalTime: 05 Nov 2005 21:54:51.0963 (UTC) FILETIME=[8CA3B8B0:01C5E253] ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dswilliams-at-ucsd.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dswilliams-at-ucsd.edu Name: DS Williams
Organization: UCSD
Title-Subject: [Filtered] POSTDOCTORAL POSITION in cell biology -at- UCSD
Question: POSTDOCTORAL POSITION in cell biology UCSD SCHOOL OF MEDICINE LA JOLLA, CALIFORNIA
Experience in microscopy required, preferably electron microscopy. Studies will be on cellular mechanisms in the retina and potential therapies for retinal degeneration. They will also include collaborative studies on other neurodegenerative disorders. See lab web site for further information about the lab: http://medicine.ucsd.edu/williams/. There will be ample opportunity to develop state-of-the-art skills and collaborations in the rich scientific environment of La Jolla. Start date: negotiable. Salary: NIH scale. Contact: Dr David Williams, Departments of Pharmacology and Neurosciences, UCSD School of Medicine, La Jolla, CA 92093-0912. Email: {DSWILLIAMS-at-UCSD.EDU}
Hi Folks, while rooting around in a cupboard for an eyepiece with graticule this morning I came across a couple of Beck "reflecting objectives" in their little mahogany boxes. I would dearly like to know what they are used for - it's clear that they focus parallel light to a point... Do they have a use in microscopy, or have I just found a component from a rather old experiment on an optical bench?
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==============================Original Headers============================== 6, 28 -- From richard.beanland-at-bookham.com Mon Nov 7 05:56:40 2005 6, 28 -- Received: from mail80.messagelabs.com (mail80.messagelabs.com [195.245.230.163]) 6, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jA7BudEk031661 6, 28 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 05:56:39 -0600 6, 28 -- X-VirusChecked: Checked 6, 28 -- X-Env-Sender: richard.beanland-at-bookham.com 6, 28 -- X-Msg-Ref: server-5.tower-80.messagelabs.com!1131364850!37240716!3 6, 28 -- X-StarScan-Version: 5.5.9.1; banners=bookham.com,-,- 6, 28 -- X-Originating-IP: [213.249.209.179] 6, 28 -- Received: (qmail 23395 invoked from network); 7 Nov 2005 12:00:52 -0000 6, 28 -- Received: from unknown (HELO cas-smx-brdg-01.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 6, 28 -- by server-5.tower-80.messagelabs.com with SMTP; 7 Nov 2005 12:00:52 -0000 6, 28 -- Content-class: urn:content-classes:message 6, 28 -- MIME-Version: 1.0 6, 28 -- Content-Type: text/plain; 6, 28 -- charset="iso-8859-1" 6, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 6, 28 -- Subject: RE: Reflecting objectives 6, 28 -- Date: Mon, 7 Nov 2005 12:00:48 -0000 6, 28 -- Message-ID: {FF719652ABB4414D86916ACAAE5BBB24015FBDF5-at-cas-smx-01.caswell1.europe.bkhm.net} 6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- Thread-Topic: Reflecting objectives 6, 28 -- Thread-Index: AcXjktIINGaVfKONQ/WQL1Hzgs2B5QAAB6Pw 6, 28 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 6, 28 -- To: {microscopy-at-microscopy.com} 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA7BudEk031661 ==============================End of - Headers==============================
Reflective objectives had several different proposes. One was an attempt to create better images as compared to the crude glass lens of the late 1800 early 1900's. They also had a purpose as they would gather and focus IR and UV. Of course you needed to use film to collect and "see" the image. I seem to remember that later ones sometimes contained a glass lens to assist in correcting for visible light images. Of course you lost all the UV /I R that the glass filtered.
Modern IR collecting objectives used in micro spectroscopy are reflective objectives revisited and we come full circle....
Frank Karl Degussa Corporation Akron Technical Center 3500 Embassy Parkway Suite 100 Akron, Ohio 44333
330-668-2235 Ext. 238
This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you.
richard.beanland-at- bookham.com To: frank.karl-at-degussa.com cc: 11/07/2005 06:58 Subject: [Microscopy] RE: Reflecting objectives AM Please respond to richard.beanland
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi Folks, while rooting around in a cupboard for an eyepiece with graticule this morning I came across a couple of Beck "reflecting objectives" in their little mahogany boxes. I would dearly like to know what they are used for - it's clear that they focus parallel light to a point... Do they have a use in microscopy, or have I just found a component from a rather old experiment on an optical bench?
======================================================================= This e-mail is intended for the person it is addressed to only. The information contained in it may be confidential and/or protected by law. If you are not the intended recipient of this message, you must not make any use of this information, or copy or show it to any person. Please contact us immediately to tell us that you have received this e-mail, and return the original to us. Any use, forwarding, printing or copying of this message is strictly prohibited. No part of this message can be considered a request for goods or services. =======================================================================
==============================Original Headers============================== 6, 28 -- From richard.beanland-at-bookham.com Mon Nov 7 05:56:40 2005 6, 28 -- Received: from mail80.messagelabs.com (mail80.messagelabs.com [195.245.230.163]) 6, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jA7BudEk031661 6, 28 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 05:56:39 -0600 6, 28 -- X-VirusChecked: Checked 6, 28 -- X-Env-Sender: richard.beanland-at-bookham.com 6, 28 -- X-Msg-Ref: server-5.tower-80.messagelabs.com!1131364850!37240716!3 6, 28 -- X-StarScan-Version: 5.5.9.1; banners=bookham.com,-,- 6, 28 -- X-Originating-IP: [213.249.209.179] 6, 28 -- Received: (qmail 23395 invoked from network); 7 Nov 2005 12:00:52 -0000 6, 28 -- Received: from unknown (HELO cas-smx-brdg-01.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 6, 28 -- by server-5.tower-80.messagelabs.com with SMTP; 7 Nov 2005 12:00:52 -0000 6, 28 -- Content-class: urn:content-classes:message 6, 28 -- MIME-Version: 1.0 6, 28 -- Content-Type: text/plain; 6, 28 -- charset="iso-8859-1" 6, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 6, 28 -- Subject: RE: Reflecting objectives 6, 28 -- Date: Mon, 7 Nov 2005 12:00:48 -0000 6, 28 -- Message-ID: {FF719652ABB4414D86916ACAAE5BBB24015FBDF5-at-cas-smx-01.caswell1.europe.bkhm.net}
6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- Thread-Topic: Reflecting objectives 6, 28 -- Thread-Index: AcXjktIINGaVfKONQ/WQL1Hzgs2B5QAAB6Pw 6, 28 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 6, 28 -- To: {microscopy-at-microscopy.com} 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA7BudEk031661 ==============================End of - Headers==============================
==============================Original Headers============================== 29, 18 -- From frank.karl-at-degussa.com Mon Nov 7 06:44:54 2005 29, 18 -- Received: from framailout1.rz.itson.com (mailout2.degussa.com [149.216.91.173]) 29, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA7Cirra008609 29, 18 -- for {microscopy-at-msa.microscopy.com} ; Mon, 7 Nov 2005 06:44:53 -0600 29, 18 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [172.20.6.74]) 29, 18 -- by framailout1.rz.itson.com (8.13.3/8.13.3/Debian-6) with ESMTP id jA7CjO45028418; 29, 18 -- Mon, 7 Nov 2005 13:45:25 +0100 29, 18 -- In-Reply-To: {200511071158.jA7BwhFV001663-at-ns.microscopy.com} 29, 18 -- Subject: Re: [Microscopy] RE: Reflecting objectives 29, 18 -- To: richard.beanland-at-bookham.com, microscopy-at-msa.microscopy.com 29, 18 -- X-Mailer: Lotus Notes Release 6.5.2 June 01, 2004 29, 18 -- Message-ID: {OF7CE559AD.2F06C2A9-ON852570B2.00459F91-852570B2.0046522B-at-degussa.com} 29, 18 -- From: frank.karl-at-degussa.com 29, 18 -- Date: Mon, 7 Nov 2005 07:48:05 -0500 29, 18 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 29, 18 -- 11/07/2005 06:49:15 AM 29, 18 -- MIME-Version: 1.0 29, 18 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
I have done work with Drosophila eyes and the following points may be of interest to you:
There are bad results if one fixes the whole head for not enough fix gets through the "neck".
UA does really increase the contrast. My way was 1%UA in the refrigerator over night (cold and dark) since specimens were usually ready to be fixed in the afternoon and I had used a combination of glut. and OsO4 on ice for fixation, but an hour or so also works.
If looking at nerves or cytoskeleton a (good quality) Acetone dehydration works better than EtOH.
The tannic acid might give some interesting results but it was not tried in my experiments. If I recall correctly when using tannic acid it was with the glut. at room temp. and freshly made but others will no doubt be better on this point.
Standard epoxy embedding is fine.
I had wanted to try the microwave techniques on the insect heads to see if the head dissection could be avoided - everything in its proper place - but did not have access to a machine until after the head experiments were over.
Lots of luck! Pat Connelly stranen_connelly-at-yahoo.com (currently seeking employment)
--- dsherman-at-purdue.edu wrote:
} We have a project coming up for preparation of } mosquito eyes for both LM and } TEM examination. I was planning on using a glut-PAF } fix in cacodylate } buffer followed by osmium tetroxide, ETOH } dehydration and embedding in epoxy } generic resin. } } Options are incubation with tannic acid and en bloc } staining with UA prior } to dehydration.
} I would appreciate some suggestions as to } preparation protocols. Have any } of you tried the tannic acid incubation? If so, at } what concentration and } when...in both glut/PAF and Os or alone after } glut/PAF? Have you used it at } RT or 4oC? Has en bloc UA been helpful in increasing } contrast in eye tissue?
} Debby Sherman, Manager Phone: } 765-494-6666 } Life Science Microscopy Facility FAX: } 765-494-5896 } Purdue University E-mail: } dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www3.agriculture.purdue.edu/microscopy
__________________________________ Yahoo! Mail - PC Magazine Editors' Choice 2005 http://mail.yahoo.com
==============================Original Headers============================== 13, 21 -- From stranen_connelly-at-yahoo.com Mon Nov 7 08:00:11 2005 13, 21 -- Received: from web34102.mail.mud.yahoo.com (web34102.mail.mud.yahoo.com [66.163.178.100]) 13, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jA7E0B4v018272 13, 21 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 08:00:11 -0600 13, 21 -- Received: (qmail 82313 invoked by uid 60001); 7 Nov 2005 14:04:37 -0000 13, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 13, 21 -- s=s1024; d=yahoo.com; 13, 21 -- h=Message-ID:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 13, 21 -- b=RrFUgOhW0eM7EGK9obaJPr3pfhneqh6Dek2l3uXKcnr9sod4NM/x9X2NJKfqyx6noYXEVAkYreMj1zzQTtUsHbAL+TSK7mvfmw3UkaIocEoqP524KwMlDK1VQh/K63PF3qIxLewDaZWZFTjMSnJGPKfF6U/H0ryIooUS8/5CKyQ= ; 13, 21 -- Message-ID: {20051107140437.82311.qmail-at-web34102.mail.mud.yahoo.com} 13, 21 -- Received: from [68.80.27.63] by web34102.mail.mud.yahoo.com via HTTP; Mon, 07 Nov 2005 06:04:37 PST 13, 21 -- Date: Mon, 7 Nov 2005 06:04:37 -0800 (PST) 13, 21 -- From: Pat Conelly {stranen_connelly-at-yahoo.com} 13, 21 -- Subject: Re: [Microscopy] Fixing insect eyes 13, 21 -- To: dsherman-at-purdue.edu 13, 21 -- Cc: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 13, 21 -- Pat Connelly {stranen_connelly-at-yahoo.com} 13, 21 -- In-Reply-To: {200511052201.jA5M1fLC021332-at-ns.microscopy.com} 13, 21 -- MIME-Version: 1.0 13, 21 -- Content-Type: text/plain; charset=iso-8859-1 13, 21 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Good Morning: We have 2 TEMs with a 3rd coming that are acquiring digital images. We would like to store these images centrally and deliver them to customers over the internet. To this end we have purchased the SIS Web Racer program but don't know how to continue. Our IT dept won't support us. If anyone out there is doing this I would much appreciate a note on how to construct such a network from the ground up. Thanks bob
Guelph Regional Imaging Facility Dept.of Molecular and Cellular Biology New Science Complex 488 Gordon St. Univ of Guelph Guelph,On, Canada N1G 2W1 ph: 519-824-4120 ext. 56409/58962 Fax: 519-837-1802
==============================Original Headers============================== 2, 26 -- From bharris-at-uoguelph.ca Mon Nov 7 10:06:18 2005 2, 26 -- Received: from moe.cs.uoguelph.ca (moe.cs.uoguelph.ca [131.104.96.55]) 2, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA7G6Ifa028807 2, 26 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 10:06:18 -0600 2, 26 -- Received: from legolas.cs.uoguelph.ca (legolas.cs.uoguelph.ca [131.104.94.45]) 2, 26 -- by moe.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id jA7G6HNI007064 2, 26 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 11:06:17 -0500 2, 26 -- Received: from legolas.cs.uoguelph.ca (localhost.localdomain [127.0.0.1]) 2, 26 -- by legolas.cs.uoguelph.ca (8.12.11/8.12.10) with ESMTP id jA7G6HOG031431 2, 26 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 11:06:17 -0500 2, 26 -- Received: (from nobody-at-localhost) 2, 26 -- by legolas.cs.uoguelph.ca (8.12.11/8.12.11/Submit) id jA7G6HbD031429 2, 26 -- for microscopy-at-microscopy.com; Mon, 7 Nov 2005 11:06:17 -0500 2, 26 -- Received: from 131.104.78.38 ([131.104.78.38]) 2, 26 -- by webmail.uoguelph.ca (IMP) with HTTP 2, 26 -- for {bharris-at-staff.mail.uoguelph.ca} ; Mon, 7 Nov 2005 11:06:17 -0500 2, 26 -- Message-ID: {1131379577.436f7b790ab2d-at-webmail.uoguelph.ca} 2, 26 -- Date: Mon, 7 Nov 2005 11:06:17 -0500 2, 26 -- From: Bob Harris {bharris-at-uoguelph.ca} 2, 26 -- To: MSA listserver {microscopy-at-microscopy.com} 2, 26 -- Subject: networking EM computers 2, 26 -- MIME-Version: 1.0 2, 26 -- Content-Type: text/plain; charset=ISO-8859-1 2, 26 -- Content-Transfer-Encoding: 8bit 2, 26 -- User-Agent: Internet Messaging Program (IMP) 3.2.4 2, 26 -- X-Scanned-By: MIMEDefang 2.52 on 131.104.96.55 ==============================End of - Headers==============================
Have at look at the Open Microscopy Environment - Jason Smedlow of OME gave a talk at UCL recently about doing such a thing using "a single relational database with XML for data migration". See the www.openmicroscopy.org . I don't know if they can provide one-to-one help but they would seem a good starting point as they have such a system up and running (and I think received a fair bit of funding to set it up).
Regards
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
----- Original Message ----- X-from: {bharris-at-uoguelph.ca} To: {keith.morris-at-ucl.ac.uk} Sent: Monday, November 07, 2005 4:14 PM
Another useful aspect of reflective objectives is that they can maintain a large NA at a long working distance and hence get better resolution than glass lenses at similar long working distance. Kind of like a telescope......
John Mardinly Intel
The copinions of the writer are not necessarily the opinions of Intel Corporation.
-----Original Message----- X-from: frank.karl-at-degussa.com [mailto:frank.karl-at-degussa.com] Sent: Monday, November 07, 2005 4:45 AM To: Mardinly, John
Reflective objectives had several different proposes. One was an attempt to create better images as compared to the crude glass lens of the late 1800 early 1900's. They also had a purpose as they would gather and focus IR and UV. Of course you needed to use film to collect and "see" the image. I seem to remember that later ones sometimes contained a glass lens to assist in correcting for visible light images. Of course you lost all the UV /I R that the glass filtered.
Modern IR collecting objectives used in micro spectroscopy are reflective objectives revisited and we come full circle....
Frank Karl Degussa Corporation Akron Technical Center 3500 Embassy Parkway Suite 100 Akron, Ohio 44333
330-668-2235 Ext. 238
This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you.
richard.beanland-at-
bookham.com To: frank.karl-at-degussa.com
cc:
11/07/2005 06:58 Subject: [Microscopy] RE: Reflecting objectives AM
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi Folks, while rooting around in a cupboard for an eyepiece with graticule this morning I came across a couple of Beck "reflecting objectives" in their little mahogany boxes. I would dearly like to know what they are used for - it's clear that they focus parallel light to a point... Do they have a use in microscopy, or have I just found a component from a rather old experiment on an optical bench?
======================================================================= This e-mail is intended for the person it is addressed to only. The information contained in it may be confidential and/or protected by law. If you are not the intended recipient of this message, you must not make any use of this information, or copy or show it to any person. Please contact us immediately to tell us that you have received this e-mail, and return the original to us. Any use, forwarding, printing or copying of this message is strictly prohibited. No part of this message can be considered a request for goods or services. =======================================================================
==============================Original Headers============================== 6, 28 -- From richard.beanland-at-bookham.com Mon Nov 7 05:56:40 2005 6, 28 -- Received: from mail80.messagelabs.com (mail80.messagelabs.com [195.245.230.163]) 6, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jA7BudEk031661 6, 28 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 05:56:39 -0600 6, 28 -- X-VirusChecked: Checked 6, 28 -- X-Env-Sender: richard.beanland-at-bookham.com 6, 28 -- X-Msg-Ref: server-5.tower-80.messagelabs.com!1131364850!37240716!3 6, 28 -- X-StarScan-Version: 5.5.9.1; banners=bookham.com,-,- 6, 28 -- X-Originating-IP: [213.249.209.179] 6, 28 -- Received: (qmail 23395 invoked from network); 7 Nov 2005 12:00:52 -0000 6, 28 -- Received: from unknown (HELO cas-smx-brdg-01.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 6, 28 -- by server-5.tower-80.messagelabs.com with SMTP; 7 Nov 2005 12:00:52 -0000 6, 28 -- Content-class: urn:content-classes:message 6, 28 -- MIME-Version: 1.0 6, 28 -- Content-Type: text/plain; 6, 28 -- charset="iso-8859-1" 6, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 6, 28 -- Subject: RE: Reflecting objectives 6, 28 -- Date: Mon, 7 Nov 2005 12:00:48 -0000 6, 28 -- Message-ID: {FF719652ABB4414D86916ACAAE5BBB24015FBDF5-at-cas-smx-01.caswell1.europe.bkh m.net}
6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- Thread-Topic: Reflecting objectives 6, 28 -- Thread-Index: AcXjktIINGaVfKONQ/WQL1Hzgs2B5QAAB6Pw 6, 28 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 6, 28 -- To: {microscopy-at-microscopy.com} 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA7BudEk031661 ==============================End of - Headers==============================
==============================Original Headers============================== 29, 18 -- From frank.karl-at-degussa.com Mon Nov 7 06:44:54 2005 29, 18 -- Received: from framailout1.rz.itson.com (mailout2.degussa.com [149.216.91.173]) 29, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA7Cirra008609 29, 18 -- for {microscopy-at-msa.microscopy.com} ; Mon, 7 Nov 2005 06:44:53 -0600 29, 18 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [172.20.6.74]) 29, 18 -- by framailout1.rz.itson.com (8.13.3/8.13.3/Debian-6) with ESMTP id jA7CjO45028418; 29, 18 -- Mon, 7 Nov 2005 13:45:25 +0100 29, 18 -- In-Reply-To: {200511071158.jA7BwhFV001663-at-ns.microscopy.com} 29, 18 -- Subject: Re: [Microscopy] RE: Reflecting objectives 29, 18 -- To: richard.beanland-at-bookham.com, microscopy-at-msa.microscopy.com 29, 18 -- X-Mailer: Lotus Notes Release 6.5.2 June 01, 2004 29, 18 -- Message-ID: {OF7CE559AD.2F06C2A9-ON852570B2.00459F91-852570B2.0046522B-at-degussa.com} 29, 18 -- From: frank.karl-at-degussa.com 29, 18 -- Date: Mon, 7 Nov 2005 07:48:05 -0500 29, 18 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 29, 18 -- 11/07/2005 06:49:15 AM 29, 18 -- MIME-Version: 1.0 29, 18 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
==============================Original Headers============================== 47, 33 -- From john.mardinly-at-intel.com Mon Nov 7 11:09:15 2005 47, 33 -- Received: from scsfmr002.sc.intel.com (fmr22.intel.com [143.183.121.14]) 47, 33 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA7H9FB1016348 47, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 7 Nov 2005 11:09:15 -0600 47, 33 -- Received: from scsfmr101.sc.intel.com (scsfmr101.sc.intel.com [10.3.253.10]) 47, 33 -- by scsfmr002.sc.intel.com (8.12.10/8.12.10/d: major-outer.mc,v 1.1 2004/09/17 17:50:56 root Exp $) with ESMTP id jA7H9E3q006276 47, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 7 Nov 2005 17:09:14 GMT 47, 33 -- Received: from scsmsxvs041.sc.intel.com (scsmsxvs041.sc.intel.com [10.3.90.10]) 47, 33 -- by scsfmr101.sc.intel.com (8.12.10/8.12.10/d: major-inner.mc,v 1.2 2004/09/17 18:05:01 root Exp $) with SMTP id jA7H769W024408 47, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 7 Nov 2005 17:07:18 GMT 47, 33 -- Received: from scsmsx332.amr.corp.intel.com ([10.3.90.6]) 47, 33 -- by scsmsxvs041.sc.intel.com (SAVSMTP 3.1.7.47) with SMTP id M2005110709091424717 47, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 07 Nov 2005 09:09:14 -0800 47, 33 -- Received: from scsmsx403.amr.corp.intel.com ([10.3.90.18]) by scsmsx332.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.211); 47, 33 -- Mon, 7 Nov 2005 09:09:14 -0800 47, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 47, 33 -- Content-class: urn:content-classes:message 47, 33 -- MIME-Version: 1.0 47, 33 -- Content-Type: text/plain; 47, 33 -- charset="us-ascii" 47, 33 -- Subject: RE: [Microscopy] Reflecting objectives 47, 33 -- Date: Mon, 7 Nov 2005 09:09:13 -0800 47, 33 -- Message-ID: {9E1ED6A623D8CB44B4866ACF20ECDB2B081EA74C-at-scsmsx403.amr.corp.intel.com} 47, 33 -- X-MS-Has-Attach: 47, 33 -- X-MS-TNEF-Correlator: 47, 33 -- Thread-Topic: [Microscopy] Reflecting objectives 47, 33 -- Thread-Index: AcXjmbLGRU26P6jWTH6eFbU8MQs8NAAI8jSQAAAZnDA= 47, 33 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 47, 33 -- To: {Microscopy-at-MSA.Microscopy.Com} 47, 33 -- X-OriginalArrivalTime: 07 Nov 2005 17:09:14.0519 (UTC) FILETIME=[FAC0CA70:01C5E3BD] 47, 33 -- X-Scanned-By: MIMEDefang 2.52 on 10.3.253.10 47, 33 -- Content-Transfer-Encoding: 8bit 47, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA7H9FB1016348 ==============================End of - Headers==============================
If your IT department won't help you, what do they actually do? What purpose do they serve? That aside, you will need some info from them. Do you already have a CAT-5 10/100BaseT Ethernet connection to the Internet? If not, you will need one. Then you will need to know if a computer connected to the cable needs a static IP or if the main server does DHCP. Then you need to know the DNS servers (primary and secondary) that your place uses (you need their IP addresses).
Then, get a simple router like Linksys BEFSR41. This is a four port switch router. Connect Internet cable to the router and the other computers to the switch ports. I assume that each PC has a NIC or built-in Ethernet. Use browser to connect to router, configure it according to info and set up a private Intranet either via DHCP or static addresses. Static is usually preferred since you can define machine names directly in c:\Windows\system32\drivers\etc\hosts, that way, everyone will get the same copy of hosts and know what is what.
One machine will always have to be on for this to work. Whichever this one is, use one of its disks as the distribution disk or connect a USB/Firewire external disk or a removable disk bay in the PC box. This will be your server PC.
All of the PCs can talk to each other and send and fetch data from the "server" PC and send to Internet destinations.
Just an idea/suggestion. But this works.
gary g.
At 08:09 AM 11/7/2005, you wrote:
} Good Morning: We have 2 TEMs with a 3rd coming that are acquiring digital } images. We would like to store these images centrally and deliver them to } customers over the internet. To this end we have purchased the SIS Web Racer } program but don't know how to continue. Our IT dept won't support } us. If anyone } out there is doing this I would much appreciate a note on how to } construct such } a network from the ground up. Thanks bob } } Guelph Regional Imaging Facility } Dept.of Molecular and Cellular } Biology } New Science Complex } 488 Gordon St. } Univ of Guelph } Guelph,On, Canada } N1G 2W1 } ph: 519-824-4120 ext. 56409/58962 } Fax: 519-837-1802 } } ==============================Original Headers============================== } 2, 26 -- From bharris-at-uoguelph.ca Mon Nov 7 10:06:18 2005 } 2, 26 -- Received: from moe.cs.uoguelph.ca (moe.cs.uoguelph.ca } [131.104.96.55]) } 2, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } jA7G6Ifa028807 } 2, 26 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 } 10:06:18 -0600 } 2, 26 -- Received: from legolas.cs.uoguelph.ca } (legolas.cs.uoguelph.ca [131.104.94.45]) } 2, 26 -- by moe.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id } jA7G6HNI007064 } 2, 26 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 } 11:06:17 -0500 } 2, 26 -- Received: from legolas.cs.uoguelph.ca } (localhost.localdomain [127.0.0.1]) } 2, 26 -- by legolas.cs.uoguelph.ca (8.12.11/8.12.10) with } ESMTP id jA7G6HOG031431 } 2, 26 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 } 11:06:17 -0500 } 2, 26 -- Received: (from nobody-at-localhost) } 2, 26 -- by legolas.cs.uoguelph.ca (8.12.11/8.12.11/Submit) } id jA7G6HbD031429 } 2, 26 -- for microscopy-at-microscopy.com; Mon, 7 Nov 2005 11:06:17 -0500 } 2, 26 -- Received: from 131.104.78.38 ([131.104.78.38]) } 2, 26 -- by webmail.uoguelph.ca (IMP) with HTTP } 2, 26 -- for {bharris-at-staff.mail.uoguelph.ca} ; Mon, 7 Nov } 2005 11:06:17 -0500 } 2, 26 -- Message-ID: {1131379577.436f7b790ab2d-at-webmail.uoguelph.ca} } 2, 26 -- Date: Mon, 7 Nov 2005 11:06:17 -0500 } 2, 26 -- From: Bob Harris {bharris-at-uoguelph.ca} } 2, 26 -- To: MSA listserver {microscopy-at-microscopy.com} } 2, 26 -- Subject: networking EM computers } 2, 26 -- MIME-Version: 1.0 } 2, 26 -- Content-Type: text/plain; charset=ISO-8859-1 } 2, 26 -- Content-Transfer-Encoding: 8bit } 2, 26 -- User-Agent: Internet Messaging Program (IMP) 3.2.4 } 2, 26 -- X-Scanned-By: MIMEDefang 2.52 on 131.104.96.55 } ==============================End of - Headers==============================
==============================Original Headers============================== 13, 25 -- From gary-at-gaugler.com Mon Nov 7 12:07:21 2005 13, 25 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 13, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jA7I7LGZ025753 13, 25 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 12:07:21 -0600 13, 25 -- Received: (qmail 32686 invoked from network); 7 Nov 2005 10:06:42 -0800 13, 25 -- Received: by simscan 1.1.0 ppid: 32672, pid: 32673, t: 3.4584s 13, 25 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1095 spam: 3.0.3 13, 25 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 13, 25 -- by qsmtp1 with SMTP; 7 Nov 2005 10:06:38 -0800 13, 25 -- Message-Id: {6.2.3.4.2.20051107095505.02946f80-at-mail.calweb.com} 13, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 13, 25 -- Date: Mon, 07 Nov 2005 10:07:20 -0800 13, 25 -- To: bharris-at-uoguelph.ca 13, 25 -- From: Gary Gaugler {gary-at-gaugler.com} 13, 25 -- Subject: Re: [Microscopy] networking EM computers 13, 25 -- Cc: MSA listserver {microscopy-at-microscopy.com} 13, 25 -- In-Reply-To: {200511071609.jA7G9Adu032183-at-ns.microscopy.com} 13, 25 -- References: {200511071609.jA7G9Adu032183-at-ns.microscopy.com} 13, 25 -- Mime-Version: 1.0 13, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 13, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on 13, 25 -- qsmtp1.mc.surewest.net 13, 25 -- X-Spam-Level: 13, 25 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 13, 25 -- version=3.0.3 ==============================End of - Headers==============================
} } Hi Folks, } while rooting around in a cupboard for an eyepiece with graticule ;this morning I came across a couple of Beck "reflecting objectives" in ;their little mahogany boxes. I would dearly like to know what they ;are used for - it's clear that they focus parallel light to a point...
;Do they have a use in microscopy, or have I just found a component ;from a rather old experiment on an optical bench?
Hi Richard
The are almost certainly microscope objectives. Put one on a scope and see how it works. The principle behind them is mirrors have almost the same focal the same focal length over a very wide range of frequencies. The small exception is explained on this Canon page on Near Field Microscopy http://www.canon.com/technology/s_labo/light/004/01.html In the Dripping Light selection it shows the a reflected wave is leaves the surface 1 wave length away from where it strikes the surface. But for most practical uses that does not effect us.
If they are low power and low NA the best way to use them for near infra red and the UV that silicone sensors will capture is to see if they will work as a prime lens for a video camera with no lenses to have different focal points at 1000 nm light and 300nm light. The lens will cover a much wider range of light than silicone will detect but you start taking about a great deal of money for cameras. You also need a monochromator or filers to make much sense of the images.
If you need higher resolution than a video camera will record. Three are some modifiable 3 mega pixel camera such as the Aiptek 5100 that has smile lens that is easy to replace that will 11 frames per second or 640 x 480 Video and a true 3 Mega pixel image on a memory card for $100. I have on my desk I am converting. I am not sure if the IR/UV filter is in the lens or on the sensor. But most of the fixed lens 3 mega pixel cameras can be modified to take other lenses and the IR/UV filters removed.
If the Breck objectives are below 40x and you are ever going to put up for surplus I want to be first on the list to be notified when you get rid of them.
Gordon Gordon Couger
I collect links on information related to light microscopes. www.couger.com/microscope/links/gclinks.html Please forward anything you think might be useful to others. Microscope Documentation is at www.science-info.org
==============================Original Headers============================== 13, 21 -- From gcc-at-couger.com Mon Nov 7 14:03:23 2005 13, 21 -- Received: from centrmmtao04.cox.net (centrmmtao04.cox.net [70.168.83.80]) 13, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA7K3Njw003630 13, 21 -- for {microscopy-at-msa.microscopy.com} ; Mon, 7 Nov 2005 14:03:23 -0600 13, 21 -- Received: from [127.0.0.1] (really [68.12.242.13]) by centrmmtao04.cox.net 13, 21 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 13, 21 -- id {20051107200222.VPBV20851.centrmmtao04.cox.net-at-[127.0.0.1]} ; 13, 21 -- Mon, 7 Nov 2005 15:02:22 -0500 13, 21 -- Message-ID: {436FB311.8030005-at-couger.com} 13, 21 -- Date: Mon, 07 Nov 2005 14:03:29 -0600 13, 21 -- From: Gordon Couger {gcc-at-couger.com} 13, 21 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 13, 21 -- X-Accept-Language: en-us, en 13, 21 -- MIME-Version: 1.0 13, 21 -- To: richard.beanland-at-bookham.com, 13, 21 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} 13, 21 -- Subject: Re: [Microscopy] RE: Reflecting objectives 13, 21 -- References: {200511071200.jA7C0TBL003930-at-ns.microscopy.com} 13, 21 -- In-Reply-To: {200511071200.jA7C0TBL003930-at-ns.microscopy.com} 13, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
A graduate student is planning to fix hepatitis C infected human liver tissue for TEM (fixation and embedding) work. I would like to know what would be the best way to fix the fresh tissue and transport it from a hospital. I am also interested to find out what precautionary measure we should take since the student will be handling infected liver tissue. We are planning to fix in glutaraldehyde in cacodylate/phosphate buffer. Is the virus considered infectious after fixation? Any suggestion will be greatly appreciated.
Thanks in advance.
Soumitra
************************************************************* Soumitra Ghoshroy College Associate Professor, Biology Director, Electron Microscopy Lab Box 3EML New Mexico State University Las Cruces, NM 88003 Tel: 505-646-3268 (office), 646-3283 (lab) Fax: 505-646-3282 e-mail:sghoshro-at-nmsu.edu http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm http://emldata.nmsu.edu/eml/
==============================Original Headers============================== 8, 20 -- From sghoshro-at-NMSU.Edu Mon Nov 7 14:18:12 2005 8, 20 -- Received: from ccserver3.nmsu.edu (ccserver3.NMSU.Edu [128.123.34.98]) 8, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA7KICnS012474 8, 20 -- for {Microscopy-at-Microscopy.Com} ; Mon, 7 Nov 2005 14:18:12 -0600 8, 20 -- Received: from ccserver3.nmsu.edu (ccserver3.nmsu.edu [127.0.0.1]) 8, 20 -- by localhost (Postfix) with SMTP id 7D60E434026 8, 20 -- for {Microscopy-at-Microscopy.Com} ; Mon, 7 Nov 2005 13:18:11 -0700 (MST) 8, 20 -- Received: from verdi (verdi.nmsu.edu [128.123.34.188]) 8, 20 -- by ccserver3.nmsu.edu (Postfix) with ESMTP id 00029404080 8, 20 -- for {Microscopy-at-Microscopy.Com} ; Mon, 7 Nov 2005 13:18:10 -0700 (MST) 8, 20 -- Date: Mon, 7 Nov 2005 13:18:10 -0700 (MST) 8, 20 -- From: sghoshro-at-NMSU.Edu 8, 20 -- X-X-Sender: sghoshro-at-verdi 8, 20 -- To: MSA Listserve {Microscopy-at-Microscopy.Com} 8, 20 -- Subject: Hepatitis C infected tissue 8, 20 -- Message-ID: {Pine.GSO.4.61.0511071313490.15539-at-verdi} 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 8, 20 -- X-PMX-Version: 5.0.3.165339, Antispam-Engine: 2.1.0.0, Antispam-Data: 2005.11.7.21 8, 20 -- X-PerlMx-Spam: Gauge=IIIIIII, Probability=7%, Report='NO_REAL_NAME 0, __C230066_P5 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_CRUFT 0' ==============================End of - Headers==============================
Leslie Krupp (Thompson) IBM Almaden Research 650 Harry Road, K19/D2 San Jose, CA 95120-6099 (408) 927-3856 ----- Forwarded by Leslie E Krupp/Almaden/IBM on 11/07/2005 02:46 PM ----- |---------+----------------------------} | | Dmrelion-at-aol.com | | | | | | 11/07/2005 02:45 | | | PM | |---------+----------------------------} } -------------------------------------------------------------------------------------------------------------------------------| | | | To: Leslie E Krupp/Almaden/IBM-at-IBMUS | | cc: | | Subject: Re: [Microscopy] Where to buy epo-tek epoxy | } -------------------------------------------------------------------------------------------------------------------------------|
Leslie,
Try going to
http://www.epotek.com/categories.asp?ID=3
The list a 353ND but their sales dept. should be able to help.
We buy their Epotek301 for use in mounting geological thin sections for cathodoluminescence studies. Good optical and thermal properties and stands up to the heat of the electron beam.
Don Marshall
Donald J. Marshall (Dr.) RELION Industries PO Box 12 Bedford, MA 01730 USA
781-275-4695 (phone) 781-271-0252 (FAX)
dmrelion-at-aol.com
http://www.excitingelectrons.com
"A weed is a flower out of place
In a message dated 11/7/2005 5:08:51 PM Eastern Standard Time, lkrupp-at-us.ibm.com writes:
Hello-
I need to purchase Epo-tek 350ND epoxy, and am having trouble finding it online. Does anyone know someone that carries it, or another contact person?
Vendor replies are fine.
Thank you, Leslie
Leslie Krupp (Thompson) IBM Almaden Research 650 Harry Road, K19/D2 San Jose, CA 95120-6099
."
==============================Original Headers============================== 25, 26 -- From lkrupp-at-us.ibm.com Mon Nov 7 16:48:18 2005 25, 26 -- Received: from e6.ny.us.ibm.com (e6.ny.us.ibm.com [32.97.182.146]) 25, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA7MmIdH032385 25, 26 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 16:48:18 -0600 25, 26 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234]) 25, 26 -- by e6.ny.us.ibm.com (8.12.11/8.12.11) with ESMTP id jA7MmHwj030882 25, 26 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 17:48:17 -0500 25, 26 -- Received: from d01av03.pok.ibm.com (d01av03.pok.ibm.com [9.56.224.217]) 25, 26 -- by d01relay02.pok.ibm.com (8.12.10/NCO/VERS6.7) with ESMTP id jA7MmHMf115012 25, 26 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 17:48:17 -0500 25, 26 -- Received: from d01av03.pok.ibm.com (loopback [127.0.0.1]) 25, 26 -- by d01av03.pok.ibm.com (8.12.11/8.13.3) with ESMTP id jA7MmHnH026158 25, 26 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 17:48:17 -0500 25, 26 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) 25, 26 -- by d01av03.pok.ibm.com (8.12.11/8.12.11) with ESMTP id jA7MmHLi026153 25, 26 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2005 17:48:17 -0500 25, 26 -- Subject: Fw: [Microscopy] Where to buy epo-tek epoxy 25, 26 -- To: microscopy-at-microscopy.com 25, 26 -- X-Mailer: Lotus Notes Release 6.0 September 26, 2002 25, 26 -- Message-ID: {OF64C69342.165D52A4-ON852570B2.007D3D1E-882570B2.007D23BB-at-us.ibm.com} 25, 26 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} 25, 26 -- Date: Mon, 7 Nov 2005 14:48:14 -0800 25, 26 -- X-MIMETrack: Serialize by Router on D01ML604/01/M/IBM(Release 7.0HF60 | October 19, 2005) at 25, 26 -- 11/07/2005 17:48:17 25, 26 -- MIME-Version: 1.0 25, 26 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gsosinsky-at-ucsd.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gsosinsky-at-ucsd.edu Name: Gina Sosinsky
Organization: University of California, San Diego
Title-Subject: [Filtered] MListserver: Position available
Question: Postdoctoral/Staff position at the National Center for Microscopy and Imaging Research at the University of California, San Diego, CA
A postdoctoral or staff position in cryo-EM and 3D reconstruction to study connexin26 wild type and mutant structures is available immediately. Our laboratory has isolated connexin26 gap junctions as in situ ordered two-dimensional crystals and as isolated hemichannels for analysis by electron microscopic structure determination, atomic force microscope imaging and biochemical studies. We are investigating the structure of wild type connexin26 connexons, functional mutants and treatments that result in changes in the pore size or shape to study structural mechanisms of connexon gating.
The successful candidate will have been awarded a Ph.D. or submitted (with oral examination pending), have experience in electron cryo-microscopy and image processing or three-dimensional reconstruction techniques. Experience in membrane purification techniques is preferred, but not essential. Excellent written and verbal skills are expected. The candidate will be working in a large interdisciplinary research center located in the University of California San Diego main campus and will have access to state of the art electron microscopes. For more information on our laboratory, please visit http://www.ncmir.ucsd.edu. Please forward this message to all appropriate personnel. Applicants should send curriculum vitae, bibliography, a brief description of present research activities and plans and the names and contact information of three references to:
Dr. Gina Sosinsky
University of California at San Diego 1070 Basic Science Building MC 0608 9500 Gilman Drive La Jolla, CA 92093-0608 858-534-0128 (office phone & voice mail) 858-534-4583 (lab phone) 858-534-7497 (fax) gsosinsky-at-ucsd.edu (email)
==============================Original Headers============================== 11, 14 -- From zaluzec-at-microscopy.com Tue Nov 8 08:09:47 2005 11, 14 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 11, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA8E9k0f023455 11, 14 -- for {microscopy-at-microscopy.com} ; Tue, 8 Nov 2005 08:09:46 -0600 11, 14 -- Mime-Version: 1.0 11, 14 -- X-Sender: (Unverified) 11, 14 -- Message-Id: {p06110402bf966200a622-at-[206.69.208.22]} 11, 14 -- Date: Tue, 8 Nov 2005 08:09:45 -0600 11, 14 -- To: microscopy-at-microscopy.com 11, 14 -- From: gsosinsky-at-ucsd.edu (by way of MicroscopyListserver) 11, 14 -- Subject: viaWWW: Postdoctoral/Staff position at the National Center for 11, 14 -- Microscopy and Imaging Research at the University of California, San 11, 14 -- Diego, CA 11, 14 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both m_jarnik-at-fccc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: m_jarnik-at-fccc.edu Name: Michal Jarnik
Organization: FCCC
Title-Subject: [Filtered] Embedding melanocytes
Question: We have some problems embedding melanocytes in Epon. Although the overall ultrastructure of the cells is typically good, the melanosomes lack the outer membrane. We tried several varieties of our standard embedding protocol (glutaraldehyde fixation followed by osmication, dehydration in EtOH and propylenoxide and embedding), but no real difference. Any suggestions would be welcome. Thanks.
Your question was outside my experience, so I contacted a virologist/microscopist I know, and she kindly sent me the following.
The required precautions sound quite serious and she suggested you contact her with any questions.
Mike ------------ Any clinical specimen should be handled with "Universal Precautions".
There is a set of guidelines known to anyone who routinely collects potentially infectious material. I would let this person place the tissue into fixative. These procedures entail the use of gloves, lab coat, face protection, special procedures for not cutting or sticking oneself with sharp objects, and use of a biosafety level 2 cabinet.
Whoever obtains the specimen from the patient (the surgeon? a pathology technologist?) can place it directly into glutaraldehyde for transport. This will render the specimen non-infectious since the agent is an enveloped virus (flavivirus). (Prion material is not killed by aldehyde fixation, but all human viruses are.) Ask the person obtaining the sample to cut it into small pieces or slivers if possible (a few mm). If you get a large chunk (e.g., a cubic cm), let it sit in glutaraldehyde overnight to ensure that the fixative gets inside. Then take your 1 cubic mm slices for EM from the surface that is well fixed. The center may or may not be well fixed, depending on how long the tissue sat before being placed into the glut. At this stage, you can handle the tissue just like any other non-infectious specimen.
Note that few good micrographs of HCV have been published, particularly from infected human liver. Also, note that flaviviruses are small (40-60 nm) enveloped RNA viruses, and that other things in the cell can resemble these particles. Most diagnoses of HCV are made histologically on the appearance of the liver, by immunostaining, and/or serologically, but not by EM. Good luck.
Sara E. Miller, Ph. D. P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-3265
----- Original Message ----- X-from: sghoshro-at-NMSU.Edu
Dear Listers,
Back in the mists of history, about two or three years ago, I put a plea for help on the listserver concerning pepper-like section contamination. This stuff resisted every attempt to get rid of it---different fixatives, four different buffer systems, various resins, five different water supplies, stains (or no stains!), fixation with and without osmium, different chemical suppliers, rewashing every piece of glassware in the lab, prayer, curses, voodoo. Even a Kitchen Witch. We tried all of the many suggestions from the dedicated denizens of the list (which normally cure any problem we have), all to no avail. It showed up in some tissues all of the time (especially retina), some tissues some of the time, some tissues never, and was often localized on membranes. We finally determined that it was osmium in part (although we got some pepper even in unosmicated samples). So I discussed the problem with a chemist who specialized in osmium chemistry. He mumbled something about changes in the air in the lab and wished us luck as he beat a hasty retreat. Must have been the haunted look on my face.
Finally, after almost two years, in desperation our lab director suggested adding 0.01M 2-mercaptoethanol to the buffer washes after the primary fixation step and in the osmium step. No more pepper. I promised if we ever figured this one out, I would share it with the list, so here you go.
The problem is that we never really figured it out, except for how to make it go away. That's great on one hand, but fundamentally frustrating on the other, since we never got a handle on why the contamination appeared suddenly in the first place and took up residence in the lab.
So, if you too have Intractable Pepper Syndrome, try the 2-ME. If you have any thoughts on why this works, we (and Tom Phillips) would love to hear them.
Relieved, but puzzled, Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 11, 23 -- From TindallR-at-missouri.edu Tue Nov 8 10:13:10 2005 11, 23 -- Received: from um-exproto8.um.umsystem.edu (um-exproto8.um.umsystem.edu [207.160.151.48]) 11, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA8GDAxC019077 11, 23 -- for {microscopy-at-microscopy.com} ; Tue, 8 Nov 2005 10:13:10 -0600 11, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto8.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 11, 23 -- Tue, 8 Nov 2005 10:13:07 -0600 11, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 23 -- Content-class: urn:content-classes:message 11, 23 -- MIME-Version: 1.0 11, 23 -- Content-Type: text/plain; 11, 23 -- charset="US-ASCII" 11, 23 -- Subject: TEM: Intractable Pepper Redux 11, 23 -- Date: Tue, 8 Nov 2005 10:13:06 -0600 11, 23 -- Message-ID: {BA876152E8653240BE8572E897083EE7980513-at-UM-EMAIL09.um.umsystem.edu} 11, 23 -- X-MS-Has-Attach: 11, 23 -- X-MS-TNEF-Correlator: 11, 23 -- Thread-Topic: TEM: Intractable Pepper Redux 11, 23 -- Thread-Index: AcXkf02M64IrfYnVQGyxnh9cFiN9RQ== 11, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 11, 23 -- To: {microscopy-at-microscopy.com} 11, 23 -- X-OriginalArrivalTime: 08 Nov 2005 16:13:07.0329 (UTC) FILETIME=[4E2A1F10:01C5E47F] 11, 23 -- Content-Transfer-Encoding: 8bit 11, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA8GDAxC019077 ==============================End of - Headers==============================
I have developed a major problem with the infamous pepper artefact. I have small intensely black dots associated with the microvilli, rough ER, Golgi, nuclear envelope, and plasma membrane. The spots vary some in size from about 6 to 30 nm. They are not on the nucleus or mucin granules except along their peripheral membranes. Tissues were incubated in vivo with a biotinylated antibody which would label an apical membrane surface antigen and then removed and rinsed in 6x in PBS. Fix in 2% PF + 0.2% glutaraldehyde + 300 nM DAPI in HWB (30 mM HEPES, 70 mM NaCl, 2 mM CaCl2, pH 7.4) for overnight at 4 C. I need the DAPI to identify the region of interest prior to dissection. Rinse in HWB + glycine 3 x 10 min. Dissect out region of interest. Block in 0.1% acetylated BSA for 45 min. Incubate the samples in a 1:10 dilution of 10 nm gold conjugated goat anti-biotin in 0.1% BSA-c in HWB for overnight at 4 C. Rinse 6x 5 min in HWB. 30 min in 25% ethanol + 0.5% uranyl acetate. 50% , 70%, 95% at 4 C, 3 x 100% ethanol for 10 min each at -20 °C. 1:1 LR Gold (no catalyst):ethanol 8 hrs at 4 °C. 2:1 LRG (no catalyst):ethanol overnight at 4 C. 100% LRG + catalyst overnight at -20 °C. 100% LRG + catalyst overnight at -20 °C. 100% LRG + catalyst for 2 hrs at -20 °C. Embed in 200 ul of fresh LRG + catalyst in sealed BEEM capsules at 4 C under UV light. Collect ultrathin sections (50–80 nm) on nickel grids. The problem is present regardless of whether I use lead or uranyl acetate to stain the thin sections.
I can't remove the pepper by floating the grids on either 0.5% HCl, 1% EDTA or 2% periodic acid for up to 60 min. I am guessing it is from the uranyl acetate en bloc but the artefact is small round dots which is not what uranyl artefacts have looked like in earlier failures. I am confident is not a gold artefact since gold would not penetrate into the cells and I can recognize a colloidal gold particle. Any thoughts on where this is coming from or how to remove it from my existing blocks would be welcome.
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
I am going to bite the bullet and try another embedding resin for a number of reasons. I want to give Quetol a try because of its reported nice contrast and ability to tolerate a small amount of water. The manufacturers data sheet gives a formulation using volume measurements (mls) of each component. I must prefer to use the more accurate and convenient method of weighing the components. Does anyone have a good Quetol formulation by weight? I googled it and found John Johnson uses: 15 gm Quetol 651 25 gm NSA 4 gm MNA 1 gm DMP-30 but others seem to have quite different proportions. Any comments?
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
We are trying to prepare XTEM samples from tinanium carbide substates, but the 180 grit SiC paper we use in the initial grinding step is just not up to taking on this extremely hard material. Any suggestions (including vendors for such abrasives)? -- Dr. Mark E. Twigg Code 6812 Naval Research Laboratory 4555 Overlook Ave., S.W. Washington DC 20375
tel: (202) 404-8543 fax: (202) 404-7194
==============================Original Headers============================== 2, 18 -- From twigg-at-estd.nrl.navy.mil Tue Nov 8 11:50:02 2005 2, 18 -- Received: from mail1.nrl.navy.mil (smail1.nrl.navy.mil [132.250.1.115]) 2, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA8Ho1Xw014229 2, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Nov 2005 11:50:01 -0600 2, 18 -- Received: from ccssun1.nrl.navy.mil (ccssun1.nrl.navy.mil [132.250.113.66]) 2, 18 -- by mail1.nrl.navy.mil (8.13.4/8.13.4) with ESMTP id jA8Ho0bR018208 2, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Nov 2005 12:50:00 -0500 (EST) 2, 18 -- Received: from [132.250.134.121] (ulfilas.nrl.navy.mil [132.250.134.121]) 2, 18 -- by ccssun1.nrl.navy.mil (8.13.1/8.13.1) with ESMTP id jA8Ho0vm000053 2, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Nov 2005 12:50:00 -0500 (EST) 2, 18 -- Mime-Version: 1.0 2, 18 -- Message-Id: {p05200f01bf96953017a7-at-[132.250.134.121]} 2, 18 -- Date: Tue, 8 Nov 2005 12:51:51 -0500 2, 18 -- To: Microscopy-at-microscopy.com 2, 18 -- From: twigg-at-estd.nrl.navy.mil 2, 18 -- Subject: Grinding Tinanium Carbide for XTEM 2, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 2, 18 -- X-Scanned-By: MIMEDefang 2.52 ==============================End of - Headers==============================
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Oh, and patience. Lots of patience.
Ron Anderson
==============================Original Headers============================== 5, 20 -- From randerson20-at-tampabay.rr.com Tue Nov 8 12:16:27 2005 5, 20 -- Received: from ms-smtp-04.tampabay.rr.com (ms-smtp-04-smtplb.tampabay.rr.com [65.32.5.134]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA8IGQeg023397 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 8 Nov 2005 12:16:27 -0600 5, 20 -- Received: from [127.0.0.1] (rrcs-24-73-73-214.se.biz.rr.com [24.73.73.214]) 5, 20 -- by ms-smtp-04.tampabay.rr.com (8.12.10/8.12.7) with ESMTP id jA8IGJai012093; 5, 20 -- Tue, 8 Nov 2005 13:16:20 -0500 (EST) 5, 20 -- Message-ID: {4370EB71.4060508-at-tampabay.rr.com} 5, 20 -- Date: Tue, 08 Nov 2005 13:16:17 -0500 5, 20 -- From: Ronald Anderson {randerson20-at-tampabay.rr.com} 5, 20 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 5, 20 -- X-Accept-Language: en-us, en 5, 20 -- MIME-Version: 1.0 5, 20 -- To: twigg-at-estd.nrl.navy.mil, Listserver {Microscopy-at-MSA.Microscopy.Com} 5, 20 -- Subject: Re: [Microscopy] Grinding Tinanium Carbide for XTEM 5, 20 -- References: {200511081750.jA8Ho8Ak014449-at-ns.microscopy.com} 5, 20 -- In-Reply-To: {200511081750.jA8Ho8Ak014449-at-ns.microscopy.com} 5, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 20 -- Content-Transfer-Encoding: 7bit 5, 20 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
Here's my formula for Quetol HARD. I've used it numerous times as a replacment for Spurr's Hard (Quetol version is slightly harder actually).
Quetol 651 17 g
NMA (nadic methyl anhydride) 23 g
DMAE / DMP-30 ( 2-dimethylaminoethanol or S-1) 0.5 g
=======================================
I am going to bite the bullet and try another embedding resin for a number of reasons. I want to give Quetol a try because of its reported nice contrast and ability to tolerate a small amount of water. The manufacturers data sheet gives a formulation using volume measurements (mls) of each component. I must prefer to use the more accurate and convenient method of weighing the components. Does anyone have a good Quetol formulation by weight? I googled it and found John Johnson uses: 15 gm Quetol 651 25 gm NSA 4 gm MNA 1 gm DMP-30 but others seem to have quite different proportions. Any comments?
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
==============================Original Headers============================== 7, 18 -- From PhillipsT-at-missouri.edu Tue Nov 8 11:40:38 2005 7, 18 -- Received: from um-exproto8.um.umsystem.edu (um-exproto8.um.umsystem.edu [207.160.151.48]) 7, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA8HecR0005324 7, 18 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 8 Nov 2005 11:40:38 -0600 7, 18 -- Received: from um-exproto8.um.umsystem.edu ([207.160.151.148]) by um-exproto8.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 7, 18 -- Tue, 8 Nov 2005 11:40:38 -0600 7, 18 -- Received: from phillips-dell.missouri.edu ([128.206.81.132]) by um-exproto8.um.umsystem.edu over TLS secured channel with Microsoft SMTPSVC(6.0.3790.1830); 7, 18 -- Tue, 8 Nov 2005 11:40:37 -0600 7, 18 -- Message-Id: {6.0.0.22.2.20051108113627.01e2a1e8-at-pop.missouri.edu} 7, 18 -- X-Sender: phillipst-at-pop.missouri.edu 7, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 6.0.0.22 7, 18 -- Date: Tue, 08 Nov 2005 11:40:35 -0600 7, 18 -- To: Microscopy-at-msa.microscopy.com 7, 18 -- From: Tom Phillips {phillipst-at-missouri.edu} 7, 18 -- Subject: Quetol 7, 18 -- Mime-Version: 1.0 7, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 18 -- X-OriginalArrivalTime: 08 Nov 2005 17:40:37.0739 (UTC) FILETIME=[87A71BB0:01C5E48B] ==============================End of - Headers==============================
Richard E. Edelmann Electron Microscopy Facility Director 362 Pearson Hall Miami University Oxford, OH 45056 Ph: 513-529-5712 Fax: 513-529-4243 HTTP://www.emf.muohio.edu
==============================Original Headers============================== 15, 17 -- From edelmare-at-muohio.edu Tue Nov 8 13:16:36 2005 15, 17 -- Received: from gw.cas.muohio.edu (cas03.cas.muohio.edu [134.53.14.2]) 15, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA8JGYog009474 15, 17 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 8 Nov 2005 13:16:35 -0600 15, 17 -- Received: from Cas_domain-MTA by gw.cas.muohio.edu 15, 17 -- with Novell_GroupWise; Tue, 08 Nov 2005 14:16:30 -0500 15, 17 -- Message-Id: {s370b33e.030-at-gw.cas.muohio.edu} 15, 17 -- X-Mailer: Novell GroupWise Internet Agent 6.5.2 15, 17 -- Date: Tue, 08 Nov 2005 14:16:05 -0500 15, 17 -- From: "RICHARD EDELMANN" {edelmare-at-muohio.edu} 15, 17 -- To: {phillipst-at-missouri.edu} 15, 17 -- Cc: {Microscopy-at-msa.microscopy.com} 15, 17 -- Subject: Re: [Microscopy] Quetol 15, 17 -- Mime-Version: 1.0 15, 17 -- Content-Type: text/plain; charset=US-ASCII 15, 17 -- Content-Transfer-Encoding: 7bit 15, 17 -- Content-Disposition: inline ==============================End of - Headers==============================
Another popular source is Struers (www.struers.com) and Lapmaster (www.lapmaster.com). They both have diamond discs (rough, 10-250 micron) and diamond films (fine, 1/4-30 micron).
Stu Smalinskas, P.E. Metallurgist SKF USA Plymouth, Michigan
--- randerson20-at-tampabay.rr.com wrote:
} Diamond lapping film on mylar substrates. Available } form a number of } vendors. We were partial to the films made by DuPont } because of their } high density of diamond particles, good particle } size control, and } adhesion of the diamonds to the substrate. This } experience is about five } years old. I'm sure that by now any number of } manufacturers can provide } films with similar properties. } } Oh, and patience. Lots of patience. } } Ron Anderson } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } twigg-at-estd.nrl.navy.mil wrote: } } } } We are trying to prepare XTEM samples from tinanium } carbide } } substates, but the 180 grit SiC paper we use in the } initial grinding } } step is just not up to taking on this extremely } hard material. Any } } suggestions (including vendors for such abrasives)?
__________________________________ Yahoo! FareChase: Search multiple travel sites in one click. http://farechase.yahoo.com
==============================Original Headers============================== 8, 19 -- From smalinskas-at-yahoo.com Tue Nov 8 14:29:56 2005 8, 19 -- Received: from web34106.mail.mud.yahoo.com (web34106.mail.mud.yahoo.com [66.163.178.104]) 8, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jA8KTuPu019320 8, 19 -- for {microscopy-at-sparc5.microscopy.com} ; Tue, 8 Nov 2005 14:29:56 -0600 8, 19 -- Received: (qmail 36894 invoked by uid 60001); 8 Nov 2005 20:29:56 -0000 8, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 8, 19 -- s=s1024; d=yahoo.com; 8, 19 -- h=Message-ID:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 8, 19 -- b=wRfpGIEGGXUffbcnkPE1B/hz2SYjsf8vE8jpVUUe4PILIuJ8IeUSQ164nRMfhUPNK+t56mRvgkAVVPd0mKXFgPilGlAOKQODZrgqw8ZtJKFczyBOIEg30j9oLDq1NMH6WSpCalAM9FQdK9iyC/9OLImEhXkHZrmLAatEWd1/HC4= ; 8, 19 -- Message-ID: {20051108202956.36892.qmail-at-web34106.mail.mud.yahoo.com} 8, 19 -- Received: from [141.151.33.213] by web34106.mail.mud.yahoo.com via HTTP; Tue, 08 Nov 2005 12:29:55 PST 8, 19 -- Date: Tue, 8 Nov 2005 12:29:55 -0800 (PST) 8, 19 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 8, 19 -- Subject: Re: [Microscopy] Re: Grinding Tinanium Carbide for XTEM 8, 19 -- To: microscopy-at-ns.microscopy.com 8, 19 -- In-Reply-To: {200511081818.jA8IIuGq026211-at-ns.microscopy.com} 8, 19 -- MIME-Version: 1.0 8, 19 -- Content-Type: text/plain; charset=iso-8859-1 8, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Our clinical diagnostic EM laboratory is considering purchasing a digital camera for our electron microscope to eliminate a lot of the wet lab photo finishing expenses. I was wondering in the hospital environment how many people out there end up printing their digital images for records purposes, rather than just storing the digital images. To me, it seems like a redundant and expense step if one already has the digital image, since that image could in fact be printed at any time.
Garry Burgess Charge Technologist Health Sciences Centre Winnipeg, Canada
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==============================Original Headers============================== 5, 20 -- From GBurgess-at-exchange.hsc.mb.ca Tue Nov 8 14:40:40 2005 5, 20 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca [142.233.100.122]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA8Kedww028149 5, 20 -- for {microscopy-at-microscopy.com} ; Tue, 8 Nov 2005 14:40:40 -0600 5, 20 -- Received: from hscxntmx0007.hsc.mb.ca (unverified [172.16.6.179]) by 5, 20 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS 4.0.346.0) with ESMTP id 5, 20 -- {B0015992781-at-hscxntmx0003.hsc.mb.ca} for {microscopy-at-microscopy.com} ;Tue, 5, 20 -- 8 Nov 2005 14:40:20 -0600 5, 20 -- Received: by hscxntmx0007.hsc.mb.ca with Internet Mail Service 5, 20 -- (5.5.2653.19)id {T7CKMZ7B} ; Tue, 8 Nov 2005 14:40:41 -0600 5, 20 -- Message-ID: 5, 20 -- {00A937989100304A83A058F6C45873FF32A2E2-at-hscxntmx0005.hsc.mb.ca} 5, 20 -- Date: Tue, 8 Nov 2005 14:38:45 -0600 5, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 5, 20 -- Subject: Printing Digital Micrographs 5, 20 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 5, 20 -- X-Mailer: Internet Mail Service (5.5.2653.19) 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; 5, 20 -- charset="iso-8859-1" ==============================End of - Headers==============================
A colleague in Chemistry would like to have some freeze-fracture work done on some liposome samples and was wondering if someone would be available to do this work and the cost per specimen.
Thank you.
JB -- ############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Email: bozzola-at-siu.edu Web: http://www.siu.edu/~image/ ##############################################################
==============================Original Headers============================== 3, 16 -- From bozzola-at-siu.edu Tue Nov 8 15:50:55 2005 3, 16 -- Received: from abbmta1.siu.edu (abbmta1.siu.edu [131.230.254.205]) 3, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA8Los3f005459 3, 16 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 8 Nov 2005 15:50:54 -0600 3, 16 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) 3, 16 -- by abbmta1.siu.edu (Switch-3.1.6/Switch-3.1.0) with ESMTP id jA8Lorit001151 3, 16 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 8 Nov 2005 15:50:54 -0600 (CST) 3, 16 -- Mime-Version: 1.0 3, 16 -- X-Sender: bozzola-at-saluki-mail.siu.edu 3, 16 -- Message-Id: {p06110410bf96cce5728b-at-[131.230.177.142]} 3, 16 -- Date: Tue, 8 Nov 2005 15:50:55 -0600 3, 16 -- To: Microscopy-at-msa.microscopy.com 3, 16 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 3, 16 -- Subject: freeze fracture contractual work needed 3, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 16 -- X-MASF: 0.00% ==============================End of - Headers==============================
Sorry, I don't know what happened with the last message, but my request was whether anyone had or knew of a surplus Sovall glass knifemaker, circa 1979, for sale. Or does anyone still service these old units? We need to replace or repair this machine, which takes 3/8" thick microtomy glass. Alternatively, are there other available knifemakers out there that will still use the thick glass?
Thanks. This question should be easier to answer, now that it's actually in the message.
Cheers, Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 8, 23 -- From TindallR-at-missouri.edu Tue Nov 8 16:53:37 2005 8, 23 -- Received: from um-exproto8.um.umsystem.edu (um-exproto8.um.umsystem.edu [207.160.151.48]) 8, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA8MrbD4024142 8, 23 -- for {microscopy-at-microscopy.com} ; Tue, 8 Nov 2005 16:53:37 -0600 8, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto8.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 8, 23 -- Tue, 8 Nov 2005 16:53:37 -0600 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="US-ASCII" 8, 23 -- Subject: Microtomy again... 8, 23 -- Date: Tue, 8 Nov 2005 16:53:37 -0600 8, 23 -- Message-ID: {BA876152E8653240BE8572E897083EE798051E-at-UM-EMAIL09.um.umsystem.edu} 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- Thread-Topic: Microtomy again... 8, 23 -- Thread-Index: AcXkt0DkVZhjaVxQRiCIQE661vwyhQ== 8, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 8, 23 -- To: {microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 08 Nov 2005 22:53:38.0028 (UTC) FILETIME=[4193CAC0:01C5E4B7] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA8MrbD4024142 ==============================End of - Headers==============================
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Question: We have been using a 1% toulidine blue O in 1% sodium borate as a general stain for all of our thick sections (1-2 microns). We use Cytoseal 60 as the mounting medium. We have noticed that the sections are fading more quickly than they have in the past and sometimes we get "round clear droplets" on the sections. Can anyone recommend a stain that won't fade so quickly? Does anyone have a clue as to the nature of the "droplets"? Thanks.
Ron is 100% correct in saying that the diamond lapping film would be suitable for this application. Having supplied this material to Ron in the past, I can say with some confidence that he is referring to a product manufactured by 3M rather than by Dupont. I would be pleased to provide you with product details as well as some guidance on your specific application. Please feel free to contact me offline.
Best regards-
David
David Henriks South Bay Techology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA
TEL =1-949-492-2600 FAX: =1-949-92-1499
email: henriks-at-southbaytech.com
-------------------------------------------------------------------------------- X-from: randerson20-at-tampabay.rr.com Sent: Tuesday, November 08, 2005 1:19 PM To: henriks-at-southbaytech.com
twigg-at-estd.nrl.navy.mil wrote:
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Oh, and patience. Lots of patience.
Ron Anderson
David Henriks South Bay Techology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA
TEL =1-949-492-2600 FAX: =1-949-92-1499
email: henriks-at-southbaytech.com
==============================Original Headers============================== 4, 23 -- From henriks-at-southbaytech.com Wed Nov 9 00:31:38 2005 4, 23 -- Received: from smtp03.smarterlinux.com (smtp03.safesecureweb.com [65.36.154.50]) 4, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA96VcpE014226 4, 23 -- for {microscopy-at-microscopy.com} ; Wed, 9 Nov 2005 00:31:38 -0600 4, 23 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 4, 23 -- by smtp03.smarterlinux.com (Postfix) with ESMTP id 4B11D38263 4, 23 -- for {microscopy-at-microscopy.com} ; Wed, 9 Nov 2005 01:31:38 -0500 (EST) 4, 23 -- Received: from mail15.safesecureweb.com (unknown [192.168.2.180]) 4, 23 -- by smtp03.smarterlinux.com (Postfix) with ESMTP id BB42F3812A 4, 23 -- for {microscopy-at-microscopy.com} ; Wed, 9 Nov 2005 01:31:37 -0500 (EST) 4, 23 -- MIME-Version: 1.0 4, 23 -- Date: Wed, 9 Nov 2005 01:31:02 -0500 4, 23 -- Content-Type: text/plain; 4, 23 -- charset=iso-8859-1 4, 23 -- Subject: Grinding Tinanium Carbide for XTEM 4, 23 -- From: "David Henriks" {henriks-at-southbaytech.com} 4, 23 -- Reply-To: henriks-at-southbaytech.com 4, 23 -- To: {microscopy-at-microscopy.com} 4, 23 -- Cc: 4, 23 -- Message-ID: {167cb3b2e9c541709143578b332b5d75-at-southbaytech.com} 4, 23 -- X-Virus-Scanned: by amavisd-new-20030616-p10 at safesecureweb.com 4, 23 -- Content-Transfer-Encoding: 8bit 4, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA96VcpE014226 ==============================End of - Headers==============================
the problem with } fading { stains (usually highly alkalinesolutions basic dyes like Toluidine Blue in borax solution, Azur II-Methylenblue-basic Fuchsin according to Richardson and others) on plastic (resin) semithin sections for me is a long known fact (regardless of the mounting media I used earlier).....so I started some 20 years ago to change my processing and archiving mode for semithin sections (we have to store the semithins of our cases for legal reasons -if possible - up to 20 years).
i) for looking at stained sections for orientation purposes, most of them also for diagnosing, as well as trimming the right location for EM-ultrathin sections we do not mount them with a slide and/or mounting medium. We find the spatial resolution already achieved WITHOUT mounting a coverslip (compared to a thick histological, paraffin-embedded section) is enough to choose the location of ultrathin section trimming.
ii) for a necessary photographic documentation or high resolution light microscopy of } seclected { stained sections we immerse the respective sections with a small drop of immersion oil, view either with a coverslide 0.17 mm placed carefully on the oildrop then (e.g. for Obj. x 4, x 10, x 25) or directly with a x 50 or x 100 oil immersion objective (Plan, NA= 1,00; 1,30, respectively....those are the good ones from the 60ies and 70ies....).
After having done this, the coverslide will be slipped away, the object slide will be immersed in (a) coplin jar(s) filled with xylol (2 x 30 sec. each) or any other diluent which will solve the immersion oil, afterwards we give the object slides a blast of compressed air (by means of a nozzle connected by a tube to a } bottle { or the pipeline of compressed air) and the mounted sections are ready for storing/archiving. If you do that in a light protected, dustfree place, you will not see - at least over 5 years from my experience - a fading of staining, also you will not have "bad wrinkles" in your sections which are produced over time due to the mounting agent.
This creates the second advantage, the first being economizing the mounting process (in avoiding permanent mounting with not very healthy mounting media as well as saving costs for coverslips and the time needed for coverslipping, drying of mounts, etc.) IF (in case this happens) the staining of sections has been gone or at least does not display brilliantly after a long period of time as you are expecting, you are able to stain again your sections quite easy and rapidly with the routine staining procedure without loss of substantial information.
Unfortunately I am really no chemist and therefore I assume a chemical reaction (de-composing solvent/material due to } aging {) which produces the "round clear droplets" you see in your mounted sections.
Best regards and wishes for a solution of your problem,
Wolfgang Muss Salzburg, Austria
PS: if you would like to have some examples of images taken digitally ith the method(s) given above, I can send them if you like (please send allowance message for sending them).
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Question: We have been using a 1% toulidine blue O in 1% sodium borate as a general stain for all of our thick sections (1-2 microns). We use Cytoseal 60 as the mounting medium. We have noticed that the sections are fading more quickly than they have in the past and sometimes we get "round clear droplets" on the sections. Can anyone recommend a stain that won't fade so quickly? Does anyone have a clue as to the nature of the "droplets"? Thanks.
I very rarely print digital images. There are two pathologists who want prints, so I just give them low resolution, plain paper prints (nothing high quality) for their own records. All images are stored in a departmental image database (Paxit). Anyone in our department has access to them. If images are to be sent out for consultation purposes, I send a CD of the tif files. In the past 2.5 years, I have printed less than 200 high quality photo paper digital images.
Ed
Edward P. Calomeni Director EM Lab The Ohio State University M018 Starling Loving Hall 320 W. 10th Ave. Columbus, OH 43210 614-293-5580 (office) 614-293-8806 (lab) edward.calomeni-at-osumc.edu -----Original Message----- X-from: GBurgess-at-exchange.hsc.mb.ca [mailto:GBurgess-at-exchange.hsc.mb.ca] Sent: Tuesday, November 08, 2005 3:46 PM To: Edward Calomeni
Our clinical diagnostic EM laboratory is considering purchasing a digital camera for our electron microscope to eliminate a lot of the wet lab photo finishing expenses. I was wondering in the hospital environment how many people out there end up printing their digital images for records purposes, rather than just storing the digital images. To me, it seems like a redundant and expense step if one already has the digital image, since that image could in fact be printed at any time.
Garry Burgess Charge Technologist Health Sciences Centre Winnipeg, Canada
This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.
==============================Original Headers============================== 5, 20 -- From GBurgess-at-exchange.hsc.mb.ca Tue Nov 8 14:40:40 2005 5, 20 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca [142.233.100.122]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA8Kedww028149 5, 20 -- for {microscopy-at-microscopy.com} ; Tue, 8 Nov 2005 14:40:40 -0600 5, 20 -- Received: from hscxntmx0007.hsc.mb.ca (unverified [172.16.6.179]) by 5, 20 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS 4.0.346.0) with ESMTP id 5, 20 -- {B0015992781-at-hscxntmx0003.hsc.mb.ca} for {microscopy-at-microscopy.com} ;Tue, 5, 20 -- 8 Nov 2005 14:40:20 -0600 5, 20 -- Received: by hscxntmx0007.hsc.mb.ca with Internet Mail Service 5, 20 -- (5.5.2653.19)id {T7CKMZ7B} ; Tue, 8 Nov 2005 14:40:41 -0600 5, 20 -- Message-ID: 5, 20 -- {00A937989100304A83A058F6C45873FF32A2E2-at-hscxntmx0005.hsc.mb.ca} 5, 20 -- Date: Tue, 8 Nov 2005 14:38:45 -0600 5, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 5, 20 -- Subject: Printing Digital Micrographs 5, 20 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 5, 20 -- X-Mailer: Internet Mail Service (5.5.2653.19) 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; 5, 20 -- charset="iso-8859-1" ==============================End of - Headers==============================
==============================Original Headers============================== 20, 31 -- From Edward.Calomeni-at-osumc.edu Wed Nov 9 07:42:02 2005 20, 31 -- Received: from pfeg01.osumc.edu (pluto.osumc.edu [140.254.120.27]) 20, 31 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA9Dg1To003986 20, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Nov 2005 07:42:01 -0600 20, 31 -- Received: from localhost (unknown [127.0.0.1]) 20, 31 -- by pfeg01.osumc.edu (Postfix) with ESMTP id 8C8BC86C6 20, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Nov 2005 13:42:01 +0000 (GMT) 20, 31 -- Received: from pfeg01.osumc.edu ([127.0.0.1]) 20, 31 -- by localhost (pfeg01.osumc.edu [127.0.0.1]) (amavisd-new, port 10024) 20, 31 -- with LMTP id 07932-01-63 for {Microscopy-at-microscopy.com} ; 20, 31 -- Wed, 9 Nov 2005 13:42:01 +0000 (GMT) 20, 31 -- Received: from msxc01.OSUMC.EDU (unknown [10.127.29.33]) 20, 31 -- by pfeg01.osumc.edu (Postfix) with ESMTP id 69FA686A3 20, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Nov 2005 13:42:01 +0000 (GMT) 20, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 20, 31 -- Content-class: urn:content-classes:message 20, 31 -- MIME-Version: 1.0 20, 31 -- Content-Type: text/plain; 20, 31 -- charset="US-ASCII" 20, 31 -- Subject: RE: [Microscopy] Printing Digital Micrographs 20, 31 -- Date: Wed, 9 Nov 2005 08:42:01 -0500 20, 31 -- Message-ID: {7A541592F9A53A4E86E7A3D5C4C7F68C27F920-at-msxc01.OSUMC.EDU} 20, 31 -- X-MS-Has-Attach: 20, 31 -- X-MS-TNEF-Correlator: 20, 31 -- Thread-Topic: [Microscopy] Printing Digital Micrographs 20, 31 -- Thread-Index: AcXkpXx/uDese8YXSbOgT67Er91ktgAjNElg 20, 31 -- From: "Edward Calomeni" {Edward.Calomeni-at-osumc.edu} 20, 31 -- To: {Microscopy-at-microscopy.com} 20, 31 -- X-Virus-Scanned: by amavisd-new at osumc.edu 20, 31 -- Content-Transfer-Encoding: 8bit 20, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA9Dg1To003986 ==============================End of - Headers==============================
We work a lot with Pathology departments in Hospitals, and our experience is that most departments are at first reluctant to the idea of not printing anymore. Prints is what the users are used to and what they know how to interpret. In fact, we often find that the Hospitals have very specific instructions about the size and paper that is to be used for printing. However, once the advantages of digital images hit the users, the use of printed images goes down. Especially, if you can equip all stations with a viewer software and have the ability to archive and transmit images to other facilities, it becomes obvious pretty quickly that prints just take a lot of time and are expensive to ship around. And it is pretty cheap to buy a couple of high quality ink jet printers for the occasional print.
However, in order to get there, you need to plan a bit ahead. Use a software that has an archiving or image database and that allows multiple users to use the archive. Make sure that you have the option to allow external users to access the database. Talk to your IT department. Keep future expansions in mind. Check to see if there are any restrictions (for example, in the US there are HIPAA laws, and the FDA may get involved with something called 21 CFR rule 11).
If you do your homework first, you can set up a system that increases your throughput quite a bit. Let me know if we can help you further with any of this.
Mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: GBurgess-at-exchange.hsc.mb.ca [mailto:GBurgess-at-exchange.hsc.mb.ca] Sent: Tuesday, November 08, 2005 1:43 PM To: Mike Bode
Our clinical diagnostic EM laboratory is considering purchasing a digital camera for our electron microscope to eliminate a lot of the wet lab photo finishing expenses. I was wondering in the hospital environment how many people out there end up printing their digital images for records purposes, rather than just storing the digital images. To me, it seems like a redundant and expense step if one already has the digital image, since that image could in fact be printed at any time.
Garry Burgess Charge Technologist Health Sciences Centre Winnipeg, Canada
This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.
==============================Original Headers============================== 5, 20 -- From GBurgess-at-exchange.hsc.mb.ca Tue Nov 8 14:40:40 2005 5, 20 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca [142.233.100.122]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA8Kedww028149 5, 20 -- for {microscopy-at-microscopy.com} ; Tue, 8 Nov 2005 14:40:40 -0600 5, 20 -- Received: from hscxntmx0007.hsc.mb.ca (unverified [172.16.6.179]) by 5, 20 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS 4.0.346.0) with ESMTP id 5, 20 -- {B0015992781-at-hscxntmx0003.hsc.mb.ca} for {microscopy-at-microscopy.com} ;Tue, 5, 20 -- 8 Nov 2005 14:40:20 -0600 5, 20 -- Received: by hscxntmx0007.hsc.mb.ca with Internet Mail Service 5, 20 -- (5.5.2653.19)id {T7CKMZ7B} ; Tue, 8 Nov 2005 14:40:41 -0600 5, 20 -- Message-ID: 5, 20 -- {00A937989100304A83A058F6C45873FF32A2E2-at-hscxntmx0005.hsc.mb.ca} 5, 20 -- Date: Tue, 8 Nov 2005 14:38:45 -0600 5, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 5, 20 -- Subject: Printing Digital Micrographs 5, 20 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 5, 20 -- X-Mailer: Internet Mail Service (5.5.2653.19) 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; 5, 20 -- charset="iso-8859-1" ==============================End of - Headers==============================
==============================Original Headers============================== 19, 23 -- From Mike.Bode-at-soft-imaging.net Wed Nov 9 09:41:26 2005 19, 23 -- Received: from mail.soft-imaging.de (mail.soft-imaging.de [62.180.61.131]) 19, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jA9FfPvK014551 19, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Nov 2005 09:41:25 -0600 19, 23 -- Received: from muenster.soft-imaging.net (muenster.soft-imaging.de [10.26.20.9]) 19, 23 -- by mail.soft-imaging.de (8.11.6/8.11.6/SuSE Linux 0.5) with ESMTP id jA9FfO506436 19, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Nov 2005 16:41:24 +0100 19, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 23 -- Content-class: urn:content-classes:message 19, 23 -- MIME-Version: 1.0 19, 23 -- Content-Type: text/plain; 19, 23 -- charset="iso-8859-1" 19, 23 -- Subject: RE: [Microscopy] Printing Digital Micrographs 19, 23 -- Date: Wed, 9 Nov 2005 16:38:53 +0100 19, 23 -- Message-ID: {6D0150089E1EA046BD96C68C50608C23023AF6C1-at-ms-s-gws.soft-imaging.net} 19, 23 -- X-MS-Has-Attach: 19, 23 -- X-MS-TNEF-Correlator: 19, 23 -- Thread-Topic: [Microscopy] Printing Digital Micrographs 19, 23 -- Thread-Index: AcXkpQszv1Sp5RWbTSqpbrrTyXIJLAAnE+fw 19, 23 -- From: "Mike Bode" {Mike.Bode-at-soft-imaging.net} 19, 23 -- To: {Microscopy-at-microscopy.com} 19, 23 -- Content-Transfer-Encoding: 8bit 19, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jA9FfPvK014551 ==============================End of - Headers==============================
Dear Stacey, The droplets are likely water from incomplete dehydration and clearing. Cytoseal is a toluene based mounting medium that will not mix with water. Extend your dehydration times and/or add an additional absolute ethanol step, and maybe add another step in clearing agent. Make sure you replace the dehydration baths and clearing baths on a regular basis as water will gradually build up in them. Although a greater issue with thicker paraffin or vibratome sections, it can occur with plastic sections if they are rushed through or the baths have not been changed.
What is the time frame of your fading? Days, months or years? Çould it be the pH of the Cytoseal? We get a few years with Toluidine Blue mounted in DPX.
Regards, Glen Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
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On Nov 8, 2005, at 5:33 PM, Stacey.Andringa-at-uc.edu wrote:
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Email: diaspro-at-fisica.unige.it Name: Alberto Diaspro
Organization: University of Genoa
Title-Subject: [Filtered] CONFOCAL 7 Meeting
Question: ELAMBS-MICROSCOBIO, DEPARTMENT OF PHYSICS, UNIVERSITY OF GENOA PROUDLY ANNOUNCES THE 7TH PRACTICAL INTENSIVE WORKSHOP ON 3D CONFOCAL MICROSCOPY ENTITLED 3D EXPLORATION OF THE LIVING WORLD
CONFOCAL 7, will be held at LAMBS, GENOA JAN 31-FEb 3, 2006.
KEY SCIENTISTS IN THE FIELD WILL GIVE TALKS ON HOT SUBJECTS IN 3D CONFOCAL MICROSCOPY INCLUDING MULTIPHOTON IMAGING, ìFî TECHNIQUES, BIOMEDICAL APPLICATIONS.
TRAINING WILL BE ON CONFOCAL AND MULTIPHOTON SPECTRAL MICROSCOPES, ULTRAFAST LASER TI-SAPPHIRE LASER SOURCES AND IMAGE DECONVOLUTION WORKSTATIONS. 4D (X-Y-Z-t), FRET, FRAP, FLIM and SHG EXPERIMENTS WILL BE PERFORMED. STUDENTS ARE ENCOURAGED TO BRING THEIR OWN SAMPLES SINCE CELL CULTURE, CHEMICAL, CELL BIOLOGY FACILITY WILL BE AVAILABLE.
Participation fees:
Academia/University Researchers 450 Ä: full School including CD and 2 lunches (Fluorescence basics on demand).
Industry/Private Researchers 600 Ä: full School including CD and 2 lunches
FREE: Tuesday afternoon Lectures, for Students, any level, and Junior Researchers.
Practical sessions will be restricted to 20 attendants.
Application: Interested candidates should send an e-mail to the Director of the Course at the following e-mail address: {mailto:diaspro-at-fisica.unige.it} diaspro-at-fisica.unige.it, using the following subject: CONFOCAL 7.
Selection will be realized on a Temporal (first-in-first-out) and Geographical (long distance applicants are favoured) basis. A short Curriculum Vitae and scientific interest of the candidate can be useful for Candidate selection.
For details see EVENTS on www.lambs.it.
--------------------------------------------- [...] Dance with wolves and count the stars, including the unseen...(L.Ferlinghetti, Challenges to young poet, 2001) --------------------------------------------- Alberto Diaspro, MicroScoBIO Research Center, LAMBS-IFOM, Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy facsimile +39-010314218 - voice +39-0103536426/480/309; URL: {http://www.lambs.it} http://www.lambs.it {http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html} http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html ----------------------------------------------
I was hoping someone out there might be able to help me out. A co-worker (not a microscopist) brought me a pair of tweezers and wants to order more but can't remember where they were originally purchased from. She thinks she bought them from a microscopy catalogue but we haven't been able to find the same ones in any of the catalogues I typically order from. They are black plastic (she think they are carbon-fiber) and the primary concern is that they are anti-static. While we can find plenty of anti-static, plastic tweezers hers are curved and our searches keep turning up straight or angled pairs. On one side is the code "J201" and the flip side has a sort of logo. It looks like a J surrounded by four "pine trees". Any assistance would be greatly appreciated.
Thanks, Angie
==============================Original Headers============================== 5, 14 -- From Anjeanette.Ormonde-at-unilever.com Thu Nov 10 09:42:43 2005 5, 14 -- Received: from mailout02.unilever.com (mailout02.unilever.com [204.110.170.4]) 5, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAAFggW4027014 5, 14 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Nov 2005 09:42:43 -0600 5, 14 -- Received: from ncrw0730.it.u4546.unilever.com ([155.197.74.207] [155.197.74.207]) by usl-004.na.unilever.com with ESMTP for Microscopy-at-microscopy.com; Thu, 10 Nov 2005 10:42:37 -0500 5, 14 -- Date: Thu, 10 Nov 2005 10:39:55 -0500 5, 14 -- From: "Anjeanette Ormonde" {Anjeanette.Ormonde-at-unilever.com} 5, 14 -- Subject: tweezers search 5, 14 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 14 -- Message-Id: {ISSMTP.2000_63_.20051110093955.115A-at-unilever.com} 5, 14 -- MIME-Version: 1.0 5, 14 -- Content-Type: TEXT/PLAIN; CHARSET=US-ASCII 5, 14 -- Content-Language: en-USA 5, 14 -- Sender: Anjeanette.Ormonde-at-unilever.com ==============================End of - Headers==============================
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I was hoping someone out there might be able to help me out. A co-worker (not a microscopist) brought me a pair of tweezers and wants to order more but can't remember where they were originally purchased from. She thinks she bought them from a microscopy catalogue but we haven't been able to find the same ones in any of the catalogues I typically order from. They are black plastic (she think they are carbon-fiber) and the primary concern is that they are anti-static. While we can find plenty of anti-static, plastic tweezers hers are curved and our searches keep turning up straight or angled pairs. On one side is the code "J201" and the flip side has a sort of logo. It looks like a J surrounded by four "pine trees". Any assistance would be greatly appreciated.
Thanks, Angie
==============================Original Headers============================== 5, 14 -- From Anjeanette.Ormonde-at-unilever.com Thu Nov 10 09:42:43 2005 5, 14 -- Received: from mailout02.unilever.com (mailout02.unilever.com [204.110.170.4]) 5, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAAFggW4027014 5, 14 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Nov 2005 09:42:43 -0600 5, 14 -- Received: from ncrw0730.it.u4546.unilever.com ([155.197.74.207] [155.197.74.207]) by usl-004.na.unilever.com with ESMTP for Microscopy-at-microscopy.com; Thu, 10 Nov 2005 10:42:37 -0500 5, 14 -- Date: Thu, 10 Nov 2005 10:39:55 -0500 5, 14 -- From: "Anjeanette Ormonde" {Anjeanette.Ormonde-at-unilever.com} 5, 14 -- Subject: tweezers search 5, 14 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 14 -- Message-Id: {ISSMTP.2000_63_.20051110093955.115A-at-unilever.com} 5, 14 -- MIME-Version: 1.0 5, 14 -- Content-Type: TEXT/PLAIN; CHARSET=US-ASCII 5, 14 -- Content-Language: en-USA 5, 14 -- Sender: Anjeanette.Ormonde-at-unilever.com ==============================End of - Headers==============================
==============================Original Headers============================== 20, 28 -- From W.Muss-at-salk.at Thu Nov 10 10:04:22 2005 20, 28 -- Received: from Hermes.salk.at (hermes.lks.at [193.170.167.9]) 20, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAAG4LZG003782 20, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Nov 2005 10:04:22 -0600 20, 28 -- Received: from localhost (localhost [127.0.0.1]) 20, 28 -- by hermes.lks.at (Postfix) with ESMTP id 5C01B5A9048; 20, 28 -- Thu, 10 Nov 2005 17:04:20 +0100 (CET) 20, 28 -- Received: from hermes.lks.at ([127.0.0.1]) 20, 28 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024) 20, 28 -- with ESMTP id 09121-07; Thu, 10 Nov 2005 17:04:20 +0100 (CET) 20, 28 -- Received: from c1pa003 (unknown [192.168.42.3]) 20, 28 -- by hermes.lks.at (Postfix) with SMTP id 332775A9044; 20, 28 -- Thu, 10 Nov 2005 17:04:20 +0100 (CET) 20, 28 -- Received: by localhost with Microsoft MAPI; Thu, 10 Nov 2005 17:04:19 +0100 20, 28 -- Message-ID: {01C5E618.C9E8ADC0.W.Muss-at-salk.at} 20, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at} 20, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at} 20, 28 -- To: "'Anjeanette.Ormonde-at-unilever.com'" {Anjeanette.Ormonde-at-unilever.com} 20, 28 -- Cc: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 20, 28 -- Subject: [Microscopy] Re: tweezers search 20, 28 -- Date: Thu, 10 Nov 2005 17:04:17 +0100 20, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at} 20, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor 20, 28 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211 20, 28 -- MIME-Version: 1.0 20, 28 -- Content-Type: text/plain; charset="us-ascii" 20, 28 -- Content-Transfer-Encoding: 7bit 20, 28 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (Willem.Wennekes-at-comcast.net) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, November 9, 2005 at 20:28:09 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both Willem.Wennekes-at-comcast.net as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: Willem.Wennekes-at-comcast.net Name: Willem Wennekes
Organization: UES
Education: Graduate College
Location: Dayton, OH, USA
Title: Dry pump emission
Question: Hi, We need to build an exhaust system for our oil pumps. The reason is the carcinogenic nature of the pump oil and therefore the exhaust. Since these pumps are used as backing pumps for systems that do not emit any other hazardous gazes, replacing these pumps with compact dry pumps seems to be a good alternative. However, scroll pumps emit graphite and we do not want to have that in the atmosphere either. I like a piston pump made by Leybold, the Ecodry M, which is advertised to have very low particle emission. Does anybody has experience with this pump or has a suggestion for a zero emission dry pump.
This Question was submitted to Ask-A-Microscopist by (foxglovelj-at-msn.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, November 10, 2005 at 14:57:05 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both foxglovelj-at-msn.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: foxglovelj-at-msn.com Name: Laura Giard
Education: Undergraduate College
Location: Sterling, MA USA
Title: microscope purchase
Question: Hello, I have a 10 year old son who wants a microscope for xmas. He wants to be a scientist when he grow up! We have purchased "cheap" microscopes over the years and have been very disappointed. I'd like to find one that truly works, however, cannot spend a fortune. Can you give me some help? Magnifications etc. do not really help me in determining the quality which is the only information I can seem to gain from places that sell them. Right now I've been looking at ones through Nasco. ANY HELP would be GREATLY APPRECIATED! Thank you.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both diller-at-stefan-diller.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: diller-at-stefan-diller.com Name: Stefan Diller
Organization: Photography
Title-Subject: [Filtered] MListserver:
Question: Dear all, does anybody have experience and / or programms to interface a KEVEX DELTA plus analyzer to a modern PC via RS232? My modell runs the Quantex + software on two Bernoulli 44MB drives. I have a second Bernoulli set available. If there is no other soultion, is there a possibility to get the Bernoulli working via SCSI on a PC to exchange the specs / data?
I would suggest the Edwards XDS5 or XDS10 dry scroll pump with a BOC Edwards XDS Silencer A50597000. The silencer is a filter trap on the output. it is available for about $200. I use both of these pumps and have no problems.
gary g.
At 03:42 PM 11/10/2005, you wrote:
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==============================Original Headers============================== 8, 25 -- From gary-at-gaugler.com Thu Nov 10 17:50:22 2005 8, 25 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 8, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jAANoMFb012739 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 10 Nov 2005 17:50:22 -0600 8, 25 -- Received: (qmail 16513 invoked from network); 10 Nov 2005 15:49:40 -0800 8, 25 -- Received: by simscan 1.1.0 ppid: 16499, pid: 16500, t: 2.6933s 8, 25 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1095 spam: 3.0.3 8, 25 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 8, 25 -- by qsmtp1 with SMTP; 10 Nov 2005 15:49:38 -0800 8, 25 -- Message-Id: {6.2.3.4.2.20051110154802.028d38b0-at-mail.calweb.com} 8, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 8, 25 -- Date: Thu, 10 Nov 2005 15:50:20 -0800 8, 25 -- To: Willem.Wennekes-at-comcast.net 8, 25 -- From: Gary Gaugler {gary-at-gaugler.com} 8, 25 -- Subject: Re: [Microscopy] AskAMicroscopist: Dry pump emission 8, 25 -- Cc: MSA listserver {microscopy-at-microscopy.com} 8, 25 -- In-Reply-To: {200511102342.jAANgYBC025408-at-ns.microscopy.com} 8, 25 -- References: {200511102342.jAANgYBC025408-at-ns.microscopy.com} 8, 25 -- Mime-Version: 1.0 8, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on 8, 25 -- qsmtp1.mc.surewest.net 8, 25 -- X-Spam-Level: 8, 25 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 8, 25 -- version=3.0.3 ==============================End of - Headers==============================
Date sent: Thu, 10 Nov 2005 17:41:26 -0600 To: r.sims-at-auckland.ac.nz X-from: Willem.Wennekes-at-comcast.net } } Question: Hi, We need to build an exhaust system for our oil pumps. } The reason is the carcinogenic nature of the pump oil and therefore } the exhaust. Since these pumps are used as backing pumps for systems } that do not emit any other hazardous gazes, replacing these pumps with } compact dry pumps seems to be a good alternative. However, scroll } pumps emit graphite and we do not want to have that in the atmosphere } either. I like a piston pump made by Leybold, the Ecodry M, which is } advertised to have very low particle emission. Does anybody has } experience with this pump or has a suggestion for a zero emission dry } pump. }
Are you sure the exhaust vapor from a rotary pump is carcinogenic?
Granted, it may not be pleasant or healthy, but as I understand it these oils are just aliphatic hydrocarbon mineral oils, and they're not carcinogenic, are they?
An oil mist trap can clean up the exhaust to the stage where it will bother no-one, particularly if discharged to out-of-doors.
Any rotary-pump-oil manufacturers listening? Any well-informed (I'm not) comment on this?
cheers
rtch
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 10, 28 -- From r.sims-at-auckland.ac.nz Thu Nov 10 18:04:59 2005 10, 28 -- Received: from smtpd.itss.auckland.ac.nz (zeppo.itss.auckland.ac.nz [130.216.190.14]) 10, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAB04wSp021611 10, 28 -- for {microscopy-at-microscopy.com} ; Thu, 10 Nov 2005 18:04:58 -0600 10, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 10, 28 -- by smtpd.itss.auckland.ac.nz (Postfix) with ESMTP id 92078345E1; 10, 28 -- Fri, 11 Nov 2005 13:04:33 +1300 (NZDT) 10, 28 -- Received: from smtpd.itss.auckland.ac.nz ([127.0.0.1]) 10, 28 -- by localhost (smtpd.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 10, 28 -- with ESMTP id 31756-13; Fri, 11 Nov 2005 13:04:33 +1300 (NZDT) 10, 28 -- Received: from n9k4q7 (glg-59-134.glg.auckland.ac.nz [130.216.59.134]) 10, 28 -- by smtpd.itss.auckland.ac.nz (Postfix) with ESMTP id 65F4D345F5; 10, 28 -- Fri, 11 Nov 2005 13:04:33 +1300 (NZDT) 10, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 10, 28 -- Organization: Dept of Geology, Univ of Auckland 10, 28 -- To: Willem.Wennekes-at-comcast.net 10, 28 -- Date: Fri, 11 Nov 2005 13:06:30 +1300 10, 28 -- MIME-Version: 1.0 10, 28 -- Subject: re: Dry pump emission 10, 28 -- Cc: microscopy-at-microscopy.com 10, 28 -- Message-ID: {43749757.23194.A8CAC9-at-localhost} 10, 28 -- Priority: normal 10, 28 -- In-reply-to: {200511102341.jAANfQTF022641-at-ns.microscopy.com} 10, 28 -- X-mailer: Pegasus Mail for Windows (4.21c) 10, 28 -- Content-type: text/plain; charset=US-ASCII 10, 28 -- Content-transfer-encoding: 7BIT 10, 28 -- Content-description: Mail message body 10, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
} Question: Hello, I have a 10 year old son who wants a microscope } for xmas. He wants to be a scientist when he grow up! We have } purchased "cheap" microscopes over the years and have been very } disappointed. I'd like to find one that truly works, however, } cannot spend a fortune. Can you give me some help? Magnifications } etc. do not really help me in determining the quality which is the } only information I can seem to gain from places that sell them. } Right now I've been looking at ones through Nasco. ANY HELP would } be GREATLY APPRECIATED! Thank you. } I urge you to look at the advice about buying microscopes on the MICRO website - URL below. You can start with MICRO's recommended 20x monocular "dissecting scope" (about $75) and go on to a basic compound scope (about $150) if he is really interested; both are needed if he's serious. Order the inspirational "Hidden Worlds - Looking Through a Scientist's Microscope" (less than $5 online), and one of the several books about simple specimen preparation listed in the MICRO bibliography, when you get the compound scope. And Email me directly if you have further questions.
Caroline
-- Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 3, 17 -- From schooley-at-mcn.org Thu Nov 10 18:24:27 2005 3, 17 -- Received: from dns4.mcn.org (dns4.mcn.org [216.150.240.31]) 3, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAB0ORVN030677 3, 17 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 10 Nov 2005 18:24:27 -0600 3, 17 -- Received: from [66.42.18.51] (helo=[10.0.1.2]) 3, 17 -- by dns4.mcn.org with esmtpa (Exim 4.43) 3, 17 -- id IPRL4P-000CP8-GF; Thu, 10 Nov 2005 16:24:26 -0800 3, 17 -- Mime-Version: 1.0 3, 17 -- Message-Id: {a06200701bf9992783585-at-[10.0.1.2]} 3, 17 -- In-Reply-To: {200511102347.jAANlaJu011788-at-ns.microscopy.com} 3, 17 -- References: {200511102347.jAANlaJu011788-at-ns.microscopy.com} 3, 17 -- Date: Thu, 10 Nov 2005 16:31:23 -0800 3, 17 -- To: foxglovelj-at-msn.com, Microscopy-at-MSA.Microscopy.Com 3, 17 -- From: Caroline Schooley {schooley-at-mcn.org} 3, 17 -- Subject: Re: [Microscopy] AskAMicroscopist: 10 year old son who wants a 3, 17 -- microscope for xmas 3, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
On Nov 10, 2005, at 3:40 PM, foxglovelj-at-msn.com wrote:
} Email: foxglovelj-at-msn.com } Name: Laura Giard } } Education: Undergraduate College } } Location: Sterling, MA USA } } Title: microscope purchase } } Question: Hello, I have a 10 year old son who wants a microscope for } xmas. He wants to be a scientist when he grow up! We have purchased } "cheap" microscopes over the years and have been very disappointed. } I'd like to find one that truly works, however, cannot spend a } fortune. Can you give me some help? Magnifications etc. do not } really help me in determining the quality which is the only } information I can seem to gain from places that sell them. Right now } I've been looking at ones through Nasco. ANY HELP would be GREATLY } APPRECIATED! Thank you. } Dear Laura, Check out Project MICRO; go to the MSA web site (www.msa.microscopy.org), click on the Reference & Educational button on the left side, and then on ProjectMICRO, the 6th item under Education Committee Functions. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 23 -- From tivol-at-caltech.edu Thu Nov 10 18:32:23 2005 4, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAB0WMRW007098 4, 23 -- for {microscopy-at-msa.microscopy.com} ; Thu, 10 Nov 2005 18:32:22 -0600 4, 23 -- Received: from localhost (earth-dog [192.168.1.3]) 4, 23 -- by water-ox-postvirus (Postfix) with ESMTP 4, 23 -- id 5C330109CE1; Thu, 10 Nov 2005 16:32:20 -0800 (PST) 4, 23 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 4, 23 -- by water-ox.its.caltech.edu (Postfix) with ESMTP 4, 23 -- id 6044833BDF; Thu, 10 Nov 2005 16:32:18 -0800 (PST) 4, 23 -- In-Reply-To: {200511102340.jAANejsU020065-at-ns.microscopy.com} 4, 23 -- References: {200511102340.jAANejsU020065-at-ns.microscopy.com} 4, 23 -- Mime-Version: 1.0 (Apple Message framework v623) 4, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 23 -- Message-Id: {42d71c1996556eb227e641f0f7616792-at-caltech.edu} 4, 23 -- Content-Transfer-Encoding: 7bit 4, 23 -- Cc: microscopy-at-msa.microscopy.com 4, 23 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 23 -- Subject: Re: [Microscopy] AskAMicroscopist: 10 year old son who wants a microscope for xmas 4, 23 -- Date: Thu, 10 Nov 2005 16:36:57 -0800 4, 23 -- To: foxglovelj-at-msn.com 4, 23 -- X-Mailer: Apple Mail (2.623) 4, 23 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.3.2 ==============================End of - Headers==============================
Good question. It is generally accepted that long term irritants (even at low levels) can be carcinogenic. For example, a jagged edge of a tooth can cause an oral cancer due to constant irritation. Oil mists (while not carcinogenic per se) can irritate the lungs and cause pneumonia or lung inflammation. Organo-metallics (and perhaps by extension, used pump oils) are known carcinogens. Used motor oil, for example, since it contains organo-metallics, is considered a low level carcinogen. I am uncertain (but suspicious, however) if used synthetic oils are similarly carcinogenic.
Microscopists can spend many hours in a room with a vacuum pump. Over a period of years, well ...... let's just say that it's worrisome. In our facility, we exhaust the oil mist from our pumps outside the building where it condenses on the roof of the building and is ultimately washed by rain into the soil. Not ideal, but the oil is diluted by water and can be broken down by microbes over time rather than being concentrated in one's lungs.
Many years ago, I evaluated an exhaust filter that trapped over 99+% of the oil mist and returned it to the pump. However, after I placed a Petri dish with distilled water in the room, I could observe (by eye as well as under a stereomicroscope) tiny droplets of oil landing on the water surface and spreading out to form an "oil slick". That's when I decided to exhaust to the outside.
Maybe the risk of lung cancer is low but why take the chance?
Cheers,
John B.
} Date sent: Thu, 10 Nov 2005 17:41:26 -0600 } To: r.sims-at-auckland.ac.nz } X-from: Willem.Wennekes-at-comcast.net } } } } Question: Hi, We need to build an exhaust system for our oil pumps. } } The reason is the carcinogenic nature of the pump oil and therefore } } the exhaust. Since these pumps are used as backing pumps for systems } } that do not emit any other hazardous gazes, replacing these pumps with } } compact dry pumps seems to be a good alternative. However, scroll } } pumps emit graphite and we do not want to have that in the atmosphere } } either. I like a piston pump made by Leybold, the Ecodry M, which is } } advertised to have very low particle emission. Does anybody has } } experience with this pump or has a suggestion for a zero emission dry } } pump. } } } } } Are you sure the exhaust vapor from a rotary pump is carcinogenic? } } Granted, it may not be pleasant or healthy, but as I understand it } these oils are just } aliphatic hydrocarbon mineral oils, and they're not carcinogenic, are they? } } An oil mist trap can clean up the exhaust to the stage where it will } bother no-one, } particularly if discharged to out-of-doors. } } Any rotary-pump-oil manufacturers listening? Any well-informed (I'm } not) comment on } this? } } cheers } } rtch } } -- } Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 } Microanalyst Fax : 64 9 3737435 } Department of Geology email : r.sims-at-auckland.ac.nz } The University of Auckland } Private Bag 92019 } Auckland } New Zealand } } } ==============================Original Headers============================== } 10, 28 -- From r.sims-at-auckland.ac.nz Thu Nov 10 18:04:59 2005 } 10, 28 -- Received: from smtpd.itss.auckland.ac.nz } (zeppo.itss.auckland.ac.nz [130.216.190.14]) } 10, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } jAB04wSp021611 } 10, 28 -- for {microscopy-at-microscopy.com} ; Thu, 10 Nov 2005 } 18:04:58 -0600 } 10, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 10, 28 -- by smtpd.itss.auckland.ac.nz (Postfix) with ESMTP id } 92078345E1; } 10, 28 -- Fri, 11 Nov 2005 13:04:33 +1300 (NZDT) } 10, 28 -- Received: from smtpd.itss.auckland.ac.nz ([127.0.0.1]) } 10, 28 -- by localhost (smtpd.itss.auckland.ac.nz [127.0.0.1]) } (amavisd-new, port 10024) } 10, 28 -- with ESMTP id 31756-13; Fri, 11 Nov 2005 13:04:33 +1300 (NZDT) } 10, 28 -- Received: from n9k4q7 (glg-59-134.glg.auckland.ac.nz } [130.216.59.134]) } 10, 28 -- by smtpd.itss.auckland.ac.nz (Postfix) with ESMTP id } 65F4D345F5; } 10, 28 -- Fri, 11 Nov 2005 13:04:33 +1300 (NZDT) } 10, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} } 10, 28 -- Organization: Dept of Geology, Univ of Auckland } 10, 28 -- To: Willem.Wennekes-at-comcast.net } 10, 28 -- Date: Fri, 11 Nov 2005 13:06:30 +1300 } 10, 28 -- MIME-Version: 1.0 } 10, 28 -- Subject: re: Dry pump emission } 10, 28 -- Cc: microscopy-at-microscopy.com } 10, 28 -- Message-ID: {43749757.23194.A8CAC9-at-localhost} } 10, 28 -- Priority: normal } 10, 28 -- In-reply-to: {200511102341.jAANfQTF022641-at-ns.microscopy.com} } 10, 28 -- X-mailer: Pegasus Mail for Windows (4.21c) } 10, 28 -- Content-type: text/plain; charset=US-ASCII } 10, 28 -- Content-transfer-encoding: 7BIT } 10, 28 -- Content-description: Mail message body } 10, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz } ==============================End of - Headers==============================
-- ############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Email: bozzola-at-siu.edu Web: http://www.siu.edu/~image/ ##############################################################
==============================Original Headers============================== 10, 18 -- From bozzola-at-siu.edu Thu Nov 10 18:48:58 2005 10, 18 -- Received: from abbmta2.siu.edu (abbmta2.siu.edu [131.230.254.206]) 10, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAB0mwgo015970 10, 18 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 10 Nov 2005 18:48:58 -0600 10, 18 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) 10, 18 -- by abbmta2.siu.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id jAB0muHj027266 10, 18 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 10 Nov 2005 18:48:57 -0600 (CST) 10, 18 -- Mime-Version: 1.0 10, 18 -- X-Sender: bozzola-at-saluki-mail.siu.edu 10, 18 -- Message-Id: {p06110420bf9993b46934-at-[131.230.177.142]} 10, 18 -- In-Reply-To: {200511110005.jAB05lVS023520-at-ns.microscopy.com} 10, 18 -- References: {200511110005.jAB05lVS023520-at-ns.microscopy.com} 10, 18 -- Date: Thu, 10 Nov 2005 18:48:57 -0600 10, 18 -- To: Microscopy-at-msa.microscopy.com 10, 18 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 10, 18 -- Subject: re: carcinogenicity of dry pump emissions 10, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 10, 18 -- X-MASF: 0.00% ==============================End of - Headers==============================
You can find some very nice scopes on ebay. Try for Olympus BH-2/BHT. Look for a unit with trinoc port and 50W or 100W lamp house. These scopes use legacy 160mm tube length objectives and can work with Oly, Nikon, Zeiss, etc. objectives. Over time, you can work up from Plan objectives to PlanAPO and add phase and DIC (hard to find). The Zeiss 63X PlanAPO was probably one of the best 160 objectives they made, IMO.
This all assumes that you want a compound LM. If you want a stereo, then look for Oly SH series scopes or Nikons. These were used extensively in semiconductor inspection and show up used routinely. But be sure that the scope has a trinoc port if you ever want to be able to take pix the best way (versus an ocular).
gary g.
At 03:42 PM 11/10/2005, you wrote:
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==============================Original Headers============================== 9, 26 -- From gary-at-gaugler.com Thu Nov 10 18:56:51 2005 9, 26 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jAB0unhe024805 9, 26 -- for {microscopy-at-microscopy.com} ; Thu, 10 Nov 2005 18:56:50 -0600 9, 26 -- Received: (qmail 1941 invoked from network); 10 Nov 2005 16:56:02 -0800 9, 26 -- Received: by simscan 1.1.0 ppid: 1923, pid: 1924, t: 4.4379s 9, 26 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1095 spam: 3.0.3 9, 26 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 9, 26 -- by qsmtp1 with SMTP; 10 Nov 2005 16:55:58 -0800 9, 26 -- Message-Id: {6.2.3.4.2.20051110163959.028b9898-at-mail.calweb.com} 9, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 9, 26 -- Date: Thu, 10 Nov 2005 16:56:40 -0800 9, 26 -- To: foxglovelj-at-msn.com 9, 26 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 26 -- Subject: Re: [Microscopy] AskAMicroscopist: 10 year old son who wants a 9, 26 -- microscope for xmas 9, 26 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 26 -- In-Reply-To: {200511102342.jAANgYGw025403-at-ns.microscopy.com} 9, 26 -- References: {200511102342.jAANgYGw025403-at-ns.microscopy.com} 9, 26 -- Mime-Version: 1.0 9, 26 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 9, 26 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on 9, 26 -- qsmtp1.mc.surewest.net 9, 26 -- X-Spam-Level: 9, 26 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 9, 26 -- version=3.0.3 ==============================End of - Headers==============================
Yes, sure, we want to avoid irritants, but I still would be careful about the "c" word, it gets tagged on to things so easily, and sticks like glue.
As you say, its the organometallics that make used motor oil a low-level carcinogen, not the base oil, and good rotary pump oils are just very pure base oils, aren't they?
And who uses synthetic oils in their rotary pumps?
Please note that I'm not advocating exposure to pump exhausts, its just the possibly careless use of "carcinogenic" that I mind.
cheers
rtch
Date sent: Thu, 10 Nov 2005 18:50:15 -0600 To: r.sims-at-auckland.ac.nz X-from: bozzola-at-siu.edu Send reply to: bozzola-at-siu.edu
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Email: christopher.hayden-at-novartis.com Name: Christopher Hayden
A sincere (and belated) thank-you to everyone who responded to my question about the fixation of mammalian eyes. We ended up going with 6% glutaraldehyde for fixation (one of the many suggested procedures). I'd love to tell everyone how it came out, but our priorities have shifted and it'll be a while before we get to actually scope the samples.
I wanted to write back to everyone indivially to thank them, but due some electronic problems I lost nearly all of my saved email(!), so it's been a fun few weeks trying to get back to "normal". We couldn't have done it as well without everyone's help.
My thanks. -Chris
-------------- Christopher Hayden PCS/Electron Microscopy Novartis Pharmaceuticals christopher.hayden-at-novartis.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both simon.ringer-at-emu.usyd.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: simon.ringer-at-emu.usyd.edu.au Name: Simon Ringer
Organization: University of Sydney
Title-Subject: [Filtered] MListserver: ACMM19
Question: Dear colleague,
I am taking the liberty of contacting you to highlight that the 19th Australian Conference for Microscopy and Microanalysis (ACMM19) will be held in Sydney, Australia, on the 5th to 9th February 2006. The biennial ACMM meetings are one of Australiaís most important forums for the microscopy and microanalytical sciences, covering the gamut of techniques and applications, and attracting a significant international attendance. More information on ACMM19 may be found on the conference web site at
http://www.acmm19.org.au.
I will take this opportunity, however, to draw your attention specifically to three points: (1) late submissions of abstracts for the conference will be accepted for a few more days; (2) a late-breaking poster session will be held at the conference; and (3) the early bird registration closes December 5th.
I, and the rest of the organising committee, look forward to a stimulating scientific interaction with you at ACMM19 in February.
Regards, Simon
Professor Simon P. Ringer Convenor, ACMM19
Director Australian Key Centre for Microscopy & Microanalysis
Executive Director Nanostructural Analysis Network Organisation Major National Research Facility NANO-MNRF www.nano.org.au
Address: Electron Microscope Unit The University of Sydney NSW, 2006 AUSTRALIA
Stefan, I have done this in the past, but it might take me a while to dig out the details. I can give you a quick synopsis now and we can talk more later.
I have used serial and ethernet communication to transfer data between the Delta and the rest of the world. I think I tried hooking up a Bernoulli drive, but then you need a utility that can read the directory under DOS/Windows. It is not a trivial thing. I received a copy of one, but I don't know that I ever got it to work.
Serial transfer should work with what you have now. You would only need a cable to plug between the two systems and maybe some software tweaks. I don't remember if the Kevex RT-11/TSX+ operating system came with a driver built in for the serial port. I think it had a driver for RT-11; I don't quite remember about TSX+. I have rebuilt copies of the operating system years ago to add different drivers, but it has been a while. Let's suppose the drivers are built in, the cable should be fairly straightforward. You may want to find someone with a breakout box to make sure the pins are all setup correctly. The Kevex doesn't hardware handshaking lines, but I think the PC does. Finally, you need some software on both ends. I used Kermit which was a rather ubiquitous program written for lots of platforms. I will have to see if I still have copies around. It supported both text and binary transfer. One end should be set up in server mode so the other computer can push or pull files around.
We went with an ethernet option because serial is slow. I purchased an ethernet card and software that allowed me to put the PDP onto an ethernet network. I think the software may only have worked under TSX, but that was okay because we usually ran under TSX than RT-11 anyway. I think I had to rebuild the operating system to add in the capability. It might be more daunting.
The next and primary question will be what will you do with the files once you have transferred them over? A lot of Kevex stuff was in a proprietary binary format. I developed procedures for transferring text output. However, spectra and images are another story. I think Kevex may have offered a spectrum export option. I developed my own utilities for converting maps and images over to TIF or BMP format.
So before spending much time hooking the two systems together, what do you want to do with the files once you can move them?
Warren
At 05:44 PM 11/10/05, you wrote:
} Email: diller-at-stefan-diller.com } Name: Stefan Diller } } Organization: Photography } } Title-Subject: [Filtered] MListserver: } } Question: Dear all, } does anybody have experience and / or programms to interface a KEVEX DELTA } plus analyzer to a modern PC via RS232? } My modell runs the Quantex + software on two Bernoulli 44MB drives. } I have a second Bernoulli set available. } If there is no other soultion, is there a possibility to get the Bernoulli } working via SCSI on a PC to exchange the specs / data? } } Thanks, } Stefan Diller
==============================Original Headers============================== 11, 21 -- From wesaia-at-iastate.edu Fri Nov 11 08:58:03 2005 11, 21 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15]) 11, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jABEw3tH005074 11, 21 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 11 Nov 2005 08:58:03 -0600 11, 21 -- Received: from mailout-1.iastate.edu (mailout-1.iastate.edu [129.186.140.1]) 11, 21 -- by mailhub-5.iastate.edu (8.12.10/8.12.10) with SMTP id jABEw24m022760; 11, 21 -- Fri, 11 Nov 2005 08:58:02 -0600 11, 21 -- Received: from strasz.marl.iastate.edu(129.186.227.11) by mailout-1.iastate.edu via csmap 11, 21 -- id 194afc06_52c5_11da_93b7_00304811d932_17950; 11, 21 -- Fri, 11 Nov 2005 09:09:04 -0600 (CST) 11, 21 -- Message-Id: {6.2.0.14.2.20051111083904.02e99ce0-at-wesaia.mail.iastate.edu} 11, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 11, 21 -- Date: Fri, 11 Nov 2005 08:56:05 -0600 11, 21 -- To: diller-at-stefan-diller.com 11, 21 -- From: Warren E Straszheim {wesaia-at-iastate.edu} 11, 21 -- Subject: Re: [Microscopy] viaWWW: KEVEX DELTA plus analyzer 11, 21 -- Cc: MSA listserver {Microscopy-at-msa.microscopy.com} 11, 21 -- In-Reply-To: {200511102344.jAANiOsW031271-at-ns.microscopy.com} 11, 21 -- References: {200511102344.jAANiOsW031271-at-ns.microscopy.com} 11, 21 -- Mime-Version: 1.0 11, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Stefan, Look at IXRF, http://www.ixrfsystems.com/index.html . They offer upgrades for Kevex systems and may have something you can use.
No interest in IXRF except as a satisfied user.
Rob Bowen -- Robert C. Bowen Research Scientist Caddock Electronics, Inc rob.bowen-at-caddock.com http://www.caddock.com
} From: {diller-at-stefan-diller.com} } Reply-To: {diller-at-stefan-diller.com} } Date: Thu, 10 Nov 2005 17:46:35 -0600 } To: {rob.bowen-at-caddock.com} } Subject: [Microscopy] viaWWW: KEVEX DELTA plus analyzer } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both diller-at-stefan-diller.com as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: diller-at-stefan-diller.com } Name: Stefan Diller } } Organization: Photography } } Title-Subject: [Filtered] MListserver: } } Question: Dear all, } does anybody have experience and / or programms to interface a KEVEX DELTA } plus analyzer to a modern PC via RS232? } My modell runs the Quantex + software on two Bernoulli 44MB drives. } I have a second Bernoulli set available. } If there is no other soultion, is there a possibility to get the Bernoulli } working via SCSI on a PC to exchange the specs / data? } } Thanks, } Stefan Diller } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 7, 12 -- From zaluzec-at-microscopy.com Thu Nov 10 17:43:34 2005 } 7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 7, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAANhWe4027752 } 7, 12 -- for {microscopy-at-microscopy.com} ; Thu, 10 Nov 2005 17:43:32 -0600 } 7, 12 -- Mime-Version: 1.0 } 7, 12 -- X-Sender: (Unverified) } 7, 12 -- Message-Id: {p06110403bf998b91a444-at-[206.69.208.22]} } 7, 12 -- Date: Thu, 10 Nov 2005 17:43:30 -0600 } 7, 12 -- To: microscopy-at-microscopy.com } 7, 12 -- From: diller-at-stefan-diller.com (by way of MicroscopyListserver) } 7, 12 -- Subject: viaWWW: KEVEX DELTA plus analyzer } 7, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 19 -- From Rob.Bowen-at-caddock.com Fri Nov 11 10:02:03 2005 6, 19 -- Received: from msg.caddock.com (msg.caddock.com [63.72.240.199]) 6, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jABG22WZ014570 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 11 Nov 2005 10:02:02 -0600 6, 19 -- Received: from [10.1.2.107] ([10.1.2.107]) 6, 19 -- by msg.caddock.com (Merak 8.2.4) with ESMTP id LSO38602; 6, 19 -- Fri, 11 Nov 2005 08:01:58 -0800 6, 19 -- User-Agent: Microsoft-Entourage/11.0.0.040405 6, 19 -- Date: Fri, 11 Nov 2005 08:01:17 -0800 6, 19 -- Subject: Re: [Microscopy] viaWWW: KEVEX DELTA plus analyzer 6, 19 -- From: Rob Bowen {Rob.Bowen-at-caddock.com} 6, 19 -- To: {diller-at-stefan-diller.com} 6, 19 -- CC: {microscopy-at-microscopy.com} 6, 19 -- Message-ID: {BF9A004D.255C%Rob.Bowen-at-caddock.com} 6, 19 -- In-Reply-To: {200511102346.jAANkZgg007784-at-ns.microscopy.com} 6, 19 -- Mime-version: 1.0 6, 19 -- Content-type: text/plain; 6, 19 -- charset="US-ASCII" 6, 19 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
A colleague here has been asked whether we can reproduce work outlined in a rather vaguely worded document that describes characterization of hydrated sections of film. Photomicrographs are shown that are said to have been acquired using a "DEH 345" penetrating electronic microscope. The data bar on the photomicrographs has a 6-digit number in the upper left corner that's preceded by the letters "ES". We wondered if this might refer to an Electroscan environmental SEM. Our searches on the instrument description haven't turned up anything. I'm hoping that the collective expertise of the listserver will shed some light on this for us!
Thank you,
Elaine
Elaine F. Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 9, 27 -- From eschumacher-at-mccrone.com Fri Nov 11 11:22:22 2005 9, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122]) 9, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jABHMMma024721 9, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 11 Nov 2005 11:22:22 -0600 9, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 9, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 326C81A8015 9, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 11 Nov 2005 11:22:24 -0600 (CST) 9, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 9, 27 -- by pgp.mccrone.com (PGP Universal service); 9, 27 -- Fri, 11 Nov 2005 11:22:24 -0600 9, 27 -- X-PGP-Universal: processed 9, 27 -- Content-class: urn:content-classes:message 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Type: text/plain; 9, 27 -- charset="US-ASCII" 9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 9, 27 -- Subject: Electron Microscope Identification 9, 27 -- Date: Fri, 11 Nov 2005 11:22:11 -0600 9, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A74C3052-at-MCCRONEMSG.tmg.mccrone.com} 9, 27 -- X-MS-Has-Attach: 9, 27 -- X-MS-TNEF-Correlator: 9, 27 -- Thread-Topic: Electron Microscope Identification 9, 27 -- Thread-Index: AcXm5HN/8milrddaTs+FUnVkyB5tcg== 9, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 9, 27 -- To: {Microscopy-at-MSA.Microscopy.Com} 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jABHMMma024721 ==============================End of - Headers==============================
Hi Everyone (Sorry if you've already seen this on the confocal microscopy list),
We are in the planning and validation stage of a Masters in Bioimaging program to be offered at Oxford Brookes University starting in Sep. 2006. This course is supported by the Royal Microscopical Society and we have gotten good feedback from industry regarding support. I am seeking input from others who have been involved in or are contemplating such a course. As part of the validation before the University committee, we need to show that such a course is viable and that there would be a reasonable demand for it. The University is doing away with those smaller Masters programs that only attract a few students per year. My feeling is that this is no problem with the renaissance in imaging that confocal microscopy has produced but I need something more concrete to present to the committee.
Specifically:
1) Are there other Graduate level courses in Bioimaging in the UK or internationally? If so, how are they doing? 2) What do you feel is the best way to advertise the course, both to new graduates and to those in industry who might like to take a module or two? 3) If you are a professional in an imaging-related company, what would you be interested in learning as a module in such a course?
I really want to hear from you whether your views are positive or otherwise. If you are in the UK and you would like to be involved as a guest lecturer, sponsor etc., by all means let me know.
Thank you.
Sincerely, John. --
********************************* C. John Runions, Ph.D. School of Biological and Molecular Sciences Oxford Brookes University Oxford, UK OX3 0BP
Used motor oil is considered carcinogenic (see 1 and 2, below).
It appears that short term exposure to oil fumes may not produce significant adverse health effects according to CITGO (see 2, below).
I can send the full references if you like. Sorry, I didn't have the time to reformat the quotes. I'll see if I can find any better refs.
Some quotes are enclosed.
1. Brief Summary of Carcinogenicity/Cancer Information: The 4- to 7-ring PAHs have been especially implicated in the carcinogenic effect of used oil [519]. Many of these same PAHs are found in new oil, although in lower concentrations [519]. Above text reprinted with permission from Environmental Toxicology and Chemistry, Volume 12(11), C. Upshall, J.F. Payne, and J. Hellou, "Induction of MFO Enzymes and Production of Bile Metabolites in Rainbow Trout (Oncorhynchus mykiss) Exposed to Waste Crankcase Oil." Copyright 1993 SETAC].
The debates on which PAHs and alkyl PAHs in complex mixtures such as this product to classify as carcinogens, and the details of exactly how to perform both ecological and human risk assessments on the complex mixtures of PAHs typically found at contaminated sites, are likely to continue. There are some clearly wrong ways to go about it, but defining clearly right ways is more difficult. PAHs usually occur in complex mixtures rather than alone.
Perhaps the most unambiguous thing that can be said about complex PAH mixtures is that such mixtures are often carcinogenic and possibly phototoxic. One way to approach site specific risk assessments would be to collect the complex mixture of PAHs and other lipophilic contaminants in a semipermeable membrane device (SPMD, also known as a fat bag) [894,895,896], retrieve the contaminant mixture from the SPMD, then test the mixture for carcinogenicity, toxicity, and phototoxicity (James Huckins, National Biological Service, and Roy Irwin, National Park Service, personal communication, 1996). Source: ENVIRONMENTAL CONTAMINANTS ENCYCLOPEDIA OIL, NEW (UNUSED MOTOR OIL) ENTRY July 1, 1997 COMPILERS/EDITORS: ROY J. IRWIN, NATIONAL PARK SERVICE WITH ASSISTANCE FROM COLORADO STATE UNIVERSITY STUDENT ASSISTANT CONTAMINANTS SPECIALISTS: MARK VAN MOUWERIK LYNETTE STEVENS MARION DUBLER SEESE WENDY BASHAM NATIONAL PARK SERVICE WATER RESOURCES DIVISIONS, WATER OPERATIONS BRANCH 1201 Oakridge Drive, Suite 250 FORT COLLINS, COLORADO 80525
2. From the CITGO MSDS (material safety data sheet): SECTION 3: HAZARDS IDENTIFICATION Inhalation No significant adverse health effects are expected to occur upon short-term exposure. CAUTION: Hot oil can cause thermal burns on contact. "Used" motor oil has been associated with skin cancer in laboratory animals following extended contact.
3. From SYNLUBE.COM: Used Motor Oil, if handled improperly and without proper personal protection and hygiene is proven to be cancer causing. Look at any back label of Petroleum or Synthetic Motor Oil made after 1985, and you will find following statement: "CAUTION: Avoid prolonged or repeated skin contact with used motor oil. Used motor oil has been shown to cause skin cancer in laboratory animals. Thoroughly wash exposed areas with soap and water."
-- ############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Email: bozzola-at-siu.edu Web: http://www.siu.edu/~image/ ##############################################################
==============================Original Headers============================== 11, 16 -- From bozzola-at-siu.edu Fri Nov 11 16:56:38 2005 11, 16 -- Received: from abbmta2.siu.edu (abbmta2.siu.edu [131.230.254.206]) 11, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jABMucZN014118 11, 16 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 11 Nov 2005 16:56:38 -0600 11, 16 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) 11, 16 -- by abbmta2.siu.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id jABMubmb027487 11, 16 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 11 Nov 2005 16:56:37 -0600 (CST) 11, 16 -- Mime-Version: 1.0 11, 16 -- X-Sender: bozzola-at-saluki-mail.siu.edu 11, 16 -- Message-Id: {p06110421bf9ac700e884-at-[131.230.177.142]} 11, 16 -- Date: Fri, 11 Nov 2005 16:56:18 -0600 11, 16 -- To: Microscopy-at-msa.microscopy.com 11, 16 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 11, 16 -- Subject: Refs: carcinogenicity of used pump oil 11, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 11, 16 -- X-MASF: 0.00% ==============================End of - Headers==============================
I would be surprised, though, if rotary pump oil, new or used, contains any PAHs, and it still seems to me that to extend the established carcinogenicity of used engine oil to rotary vacuum pump oil is a bit of a stretch, isn't it?
Of course it is obviously best to play safe with exposure to any chemical products, but I don't think we should label something as carcinogenic without fairly good cause.
cheers
rtch
Date sent: Fri, 11 Nov 2005 16:58:07 -0600 To: r.sims-at-auckland.ac.nz X-from: bozzola-at-siu.edu Send reply to: bozzola-at-siu.edu
Thanks to everyone who responded to my inquiry regarding fixation of Hepatitis C infected tissue. We revceived some great responses.
Best wishes,
Soumitra
************************************************************* Soumitra Ghoshroy College Associate Professor, Biology Director, Electron Microscopy Lab Box 3EML New Mexico State University Las Cruces, NM 88003 Tel: 505-646-3268 (office), 646-3283 (lab) Fax: 505-646-3282 e-mail:sghoshro-at-nmsu.edu http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm http://emldata.nmsu.edu/eml/
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Presumably the Al2O3 granules used in Edwards and other foreline traps can be regenerated by heating in a furnace.
What temperature and time is necessary to bake/burn off trapped oil without destroying the physical structure so that Al2O3 dust goes into the rotary pump?
cheers
rtch
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 7, 27 -- From r.sims-at-auckland.ac.nz Fri Nov 11 17:34:18 2005 7, 27 -- Received: from smtpc.itss.auckland.ac.nz (mailhost.auckland.ac.nz [130.216.190.13]) 7, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jABNYHFc007892 7, 27 -- for {Microscopy-at-Microscopy.Com} ; Fri, 11 Nov 2005 17:34:18 -0600 7, 27 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 7, 27 -- by smtpc.itss.auckland.ac.nz (Postfix) with ESMTP id 1D4AB34319 7, 27 -- for {Microscopy-at-Microscopy.Com} ; Sat, 12 Nov 2005 12:34:17 +1300 (NZDT) 7, 27 -- Received: from smtpc.itss.auckland.ac.nz ([127.0.0.1]) 7, 27 -- by localhost (smtpc.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 7, 27 -- with ESMTP id 00767-03 for {Microscopy-at-Microscopy.Com} ; 7, 27 -- Sat, 12 Nov 2005 12:34:17 +1300 (NZDT) 7, 27 -- Received: from n9k4q7 (glg-59-134.glg.auckland.ac.nz [130.216.59.134]) 7, 27 -- by smtpc.itss.auckland.ac.nz (Postfix) with ESMTP id F089133F48 7, 27 -- for {Microscopy-at-Microscopy.Com} ; Sat, 12 Nov 2005 12:34:16 +1300 (NZDT) 7, 27 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 7, 27 -- Organization: Dept of Geology, Univ of Auckland 7, 27 -- To: Microscopy-at-Microscopy.Com 7, 27 -- Date: Sat, 12 Nov 2005 12:36:15 +1300 7, 27 -- MIME-Version: 1.0 7, 27 -- Subject: Regenation of Al2O3 granules 7, 27 -- Message-ID: {4375E1C3.17256.5B3B435-at-localhost} 7, 27 -- Priority: normal 7, 27 -- X-mailer: Pegasus Mail for Windows (4.21c) 7, 27 -- Content-type: text/plain; charset=US-ASCII 7, 27 -- Content-transfer-encoding: 7BIT 7, 27 -- Content-description: Mail message body 7, 27 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
Of course there's no way we can make alumina into royalty, I meant "Regeneration"
cheers
rtch
------- Forwarded message follows ------- Date sent: Fri, 11 Nov 2005 17:35:00 -0600 To: r.sims-at-auckland.ac.nz X-from: r.sims-at-auckland.ac.nz Send reply to: r.sims-at-auckland.ac.nz
There seems to be a leap from one subject to another here. Used motor oil is just that, motor oil that was used in an internal combustion engine where a thin film of the oil with a very high surface area has been repeatedly exposed to the high temp, high pressure combustion conditions inside the cylinders. That oil is exposed to transitory, highly reactive species and highly poisonous species including free radicals, nitrogen oxides, carbon monoxide, and a host of partially oxidized hydrocarbons and water vapor. Catalytic converters are used downstream to destroy the more troublesome persistent species from the exhaust but the oil absorbs some of them and cycles them back through the cylinders over and over. These species are also free to react with finely divided metal particles and detergent additives in the oil. Its an environment where a plethora of new species are created and, once created, allowed to react with each other. The high pressure also influences the situation by increasing the tendency to force gaseous species into solution in the oil, prolonging their opportunities to react, rather than encouraging their vapor phase dilution.
Whether any chemistry is going on at all in vacuum pump oil depends on what is being exhausted. But it's safe to say that many lab pumps are not run under conditions that make them a pressure reactor like the one that exists in every internal combustion engine.
John Twilley
-----Original Message----- X-from: bozzola-at-siu.edu Sent: Nov 11, 2005 5:57 PM To: jtwilley-at-sprynet.com
Used motor oil is considered carcinogenic (see 1 and 2, below).
It appears that short term exposure to oil fumes may not produce significant adverse health effects according to CITGO (see 2, below).
I can send the full references if you like. Sorry, I didn't have the time to reformat the quotes. I'll see if I can find any better refs.
Some quotes are enclosed.
1. Brief Summary of Carcinogenicity/Cancer Information: The 4- to 7-ring PAHs have been especially implicated in the carcinogenic effect of used oil [519]. Many of these same PAHs are found in new oil, although in lower concentrations [519]. Above text reprinted with permission from Environmental Toxicology and Chemistry, Volume 12(11), C. Upshall, J.F. Payne, and J. Hellou, "Induction of MFO Enzymes and Production of Bile Metabolites in Rainbow Trout (Oncorhynchus mykiss) Exposed to Waste Crankcase Oil." Copyright 1993 SETAC].
The debates on which PAHs and alkyl PAHs in complex mixtures such as this product to classify as carcinogens, and the details of exactly how to perform both ecological and human risk assessments on the complex mixtures of PAHs typically found at contaminated sites, are likely to continue. There are some clearly wrong ways to go about it, but defining clearly right ways is more difficult. PAHs usually occur in complex mixtures rather than alone.
Perhaps the most unambiguous thing that can be said about complex PAH mixtures is that such mixtures are often carcinogenic and possibly phototoxic. One way to approach site specific risk assessments would be to collect the complex mixture of PAHs and other lipophilic contaminants in a semipermeable membrane device (SPMD, also known as a fat bag) [894,895,896], retrieve the contaminant mixture from the SPMD, then test the mixture for carcinogenicity, toxicity, and phototoxicity (James Huckins, National Biological Service, and Roy Irwin, National Park Service, personal communication, 1996). Source: ENVIRONMENTAL CONTAMINANTS ENCYCLOPEDIA OIL, NEW (UNUSED MOTOR OIL) ENTRY July 1, 1997 COMPILERS/EDITORS: ROY J. IRWIN, NATIONAL PARK SERVICE WITH ASSISTANCE FROM COLORADO STATE UNIVERSITY STUDENT ASSISTANT CONTAMINANTS SPECIALISTS: MARK VAN MOUWERIK LYNETTE STEVENS MARION DUBLER SEESE WENDY BASHAM NATIONAL PARK SERVICE WATER RESOURCES DIVISIONS, WATER OPERATIONS BRANCH 1201 Oakridge Drive, Suite 250 FORT COLLINS, COLORADO 80525
2. From the CITGO MSDS (material safety data sheet): SECTION 3: HAZARDS IDENTIFICATION Inhalation No significant adverse health effects are expected to occur upon short-term exposure. CAUTION: Hot oil can cause thermal burns on contact. "Used" motor oil has been associated with skin cancer in laboratory animals following extended contact.
3. From SYNLUBE.COM: Used Motor Oil, if handled improperly and without proper personal protection and hygiene is proven to be cancer causing. Look at any back label of Petroleum or Synthetic Motor Oil made after 1985, and you will find following statement: "CAUTION: Avoid prolonged or repeated skin contact with used motor oil. Used motor oil has been shown to cause skin cancer in laboratory animals. Thoroughly wash exposed areas with soap and water."
-- ############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Email: bozzola-at-siu.edu Web: http://www.siu.edu/~image/ ##############################################################
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==============================Original Headers============================== 21, 18 -- From jtwilley-at-sprynet.com Fri Nov 11 20:10:13 2005 21, 18 -- Received: from pop04.mail.atl.earthlink.net (pop04.mail.atl.earthlink.net [207.69.200.28]) 21, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAC2ADRl027388 21, 18 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 11 Nov 2005 20:10:13 -0600 21, 18 -- Received: from mswamui-swiss.atl.sa.earthlink.net ([209.86.224.50]) 21, 18 -- by pop04.mail.atl.earthlink.net with esmtp (Exim 3.36 #10) 21, 18 -- id 1EakqK-0004ws-00; Fri, 11 Nov 2005 21:10:12 -0500 21, 18 -- Message-ID: {21948471.1131761412105.JavaMail.root-at-mswamui-swiss.atl.sa.earthlink.net} 21, 18 -- Date: Fri, 11 Nov 2005 21:10:11 -0500 (GMT-05:00) 21, 18 -- From: jtwilley-at-sprynet.com 21, 18 -- Reply-To: jtwilley-at-sprynet.com 21, 18 -- To: bozzola-at-siu.edu, Microscopy-at-msa.microscopy.com 21, 18 -- Subject: Re: [Microscopy] Refs: carcinogenicity of used pump oil 21, 18 -- Mime-Version: 1.0 21, 18 -- Content-Type: text/plain; charset=us-ascii 21, 18 -- X-Mailer: EarthLink Zoo Mail 1.0 21, 18 -- Content-Transfer-Encoding: 8bit 21, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAC2ADRl027388 ==============================End of - Headers==============================
I'll just point out that a good STARTING point when ever you are unsure and want to find out the generic hazards of a material are the Materials Safety Data Sheets (MSDS).
These are required by virtually all safety departments in most organziations and are also usually shipped with materials or are available on-line from the manufacturer.
You can also MSDSs on-line at various WWW sites here is a list of starting points. In general it is a good policy to have access to data sheets on all chemcials you use in your lab.
http://www.ilpi.com/msds/#What
Most of the sites listed above are free, but a few require registration.
FYI appended is a short excerpt on "Edwards High Vacuum Mechanical Pump Oil" which is a pretty common mechanical pump oil around EM Labs. You will notice it says this particular material is not carcinogenitic, although it does not discuss "used" oil. It does recommend avoiding the mist, which is a good policy.
Having said this, I will concur with others that venting roughing pump systems to areas outside the immediate laboratory area is generally a good idea. For the most part we exhaust roughing pumps using simple PVC piping into fume hoods (some of which are a good distance away ~ 100 ft) the exhaust of those hoods are filtered and and NOT released into the immediate laboratory air. I prefer this to simple mist filters that are mounted on the exhaust port of the pumps. Admittedly this costs more, but it is remember that you or your colleague may end up spending "years" in a lab and it makes more sense to be cautious rather than cheap.
======================================================= Health Hazards Data ======================================================= LD50 LC50 Mixture: ORAL LD50(RAT): } 5 ML/KG Route Of Entry Inds - Inhalation: YES Skin: NO Ingestion: YES Carcinogenicity Inds - NTP: NO IARC: NO OSHA: NO Effects of Exposure: SKIN: DERMATITIS & ERYTHEMA. EYES: IRRITATION OF THE CONJUNCTIVA. INHALATION: MAY CAUSE A CHRONIC INFLAMMATORY REACTION OF THE LUNGS & A FORM OF PULMONARY FIBROSIS. INGESTION: ASPIRATION OF LIQUID INT O THE LUNGS COULD CAUSE PNEUMONIA. Explanation Of Carcinogenicity: NONE Signs And Symptions Of Overexposure: DEFATTING, OIL ACNE, OIL FOLICULITIS, IRRITATION. First Aid: SKIN/EYES: WASH W/COPIOUS AMOUNTS OF WATER FOR 10 MINS. INHALATION: REMOVE TO FRESH AIR. INGESTION: DON'T INDUCE VOMITING. SEND TO HOSPITAL IMMEDIATELY. OBTAIN MEDICAL ATTENTION IN ALL CASES. ======================================================= Handling and Disposal ======================================================= Spill Release Procedures: PREVENT ENTRY TO DRAINS & WATERCOURSES. LARGE: USE A SUITABLE MEDIUM SUCH AS SAND/EARTH. RECLAIM LIQUID DIRECTLY/IN AN ABSORBENT MEDIUM THEN TRANSFER TO SUITABLE MARKED CONTAINERS & DISPOSE OF. SMALL: SOAK UP W/SAND/EARTH & DISPOSE OF. Waste Disposal Methods: DISPOSE OF TO A LICENSED WASTE CONTRACTOR, IAW/FEDERAL, STATE & LOCAL REGULATIONS. Handling And Storage Precautions: OIL MIST SHOULD BE KEPT TO A MINIMUM, PREFERABLY WELL {5 MG/CUM. STORE AWAY FROM DIRECT HEAT. Other Precautions: AVOID OIL MIST FUMES & VAPORS. ======================================================= Fire and Explosion Hazard Information ======================================================= Flash Point Method: PMCC Flash Point Text: 460.4F Extinguishing Media: FOAM, DRY POWDER, CO2, HALON. Fire Fighting Procedures: DON'T USE WATER JETS. WEAR A SCBA IN CONFINED AREAS & FOR ANY SIGNIFICANT FIRE. Unusual Fire/Explosion Hazard: AUTOIGNITION TEMP: 608F. THERE IS A POSSIBILITY OF EXPLOSION IF YOU PUMP GASES CONTAINING OXYGEN AT A CONCENTRATION } 4% ABOVE THE PROPORTION CONTAINED IN AIR. ======================================================= Control Measures ======================================================= Respiratory Protection: NOT REQUIRED UNDER NORMAL CONDITIONS. Ventilation: LOCAL EXHAUST W/MIST FILTER, EXHAUST TO ATMOSPHERE. Protective Gloves: IMPERVIOUS POLY VINYL CHLORIDE Eye Protection: NOT NECESSARY Other Protective Equipment: OVERALLS.
Does anyone know of a rotatable specimen holder for "standard" pin stubs? What I would like is a holder to take 3mm pin stubs and be able to externally rotate it on the main specimen holder.
The application is that the specimen is on a 45 degree pre-tilt holder. Then, I want to be able to rotate the specimen using an external joystick or knob. The unit cannot be too tall or it won't fit through my specimen load lock.
Any ideas?
gary g.
==============================Original Headers============================== 6, 21 -- From gary-at-gaugler.com Sun Nov 13 13:17:11 2005 6, 21 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jADJHAQH001563 6, 21 -- for {microscopy-at-microscopy.com} ; Sun, 13 Nov 2005 13:17:11 -0600 6, 21 -- Received: (qmail 9637 invoked from network); 13 Nov 2005 11:16:41 -0800 6, 21 -- Received: by simscan 1.1.0 ppid: 9631, pid: 9632, t: 0.8577s 6, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1162 spam: 3.0.3 6, 21 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 6, 21 -- by qsmtp3 with SMTP; 13 Nov 2005 11:16:40 -0800 6, 21 -- Message-Id: {6.2.3.4.2.20051113111330.02890330-at-mail.calweb.com} 6, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 6, 21 -- Date: Sun, 13 Nov 2005 11:17:12 -0800 6, 21 -- To: MSA listserver {microscopy-at-microscopy.com} 6, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 6, 21 -- Subject: specimen rotation unit 6, 21 -- Mime-Version: 1.0 6, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 21 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp3.surewest.net 6, 21 -- X-Spam-Level: 6, 21 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 6, 21 -- version=3.0.3 ==============================End of - Headers==============================
It looks like we'll be getting the funds for a new SEM in the near future (fingers crossed!!) and we are thinking of going down the field emission, variable pressure, natural, ESEM route.
I have contacts that are using some of the available systems, but there are no Hitachi S4300SE/N systems in Australia yet. If anyone out there has had experience with these machines I would appreciate some feedback on performance, ease of use etc.. Please feel free to email me personally with any comments.
Just for the record, we are currently using a Hitachi S4100.
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==============================Original Headers============================== 16, 27 -- From Colin.Veitch-at-csiro.au Sun Nov 13 17:05:58 2005 16, 27 -- Received: from vic-ironport-ext-out3.csiro.au (vic-ironport-ext-out3.csiro.au [150.229.64.39]) 16, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jADN5vkk012868 16, 27 -- for {microscopy-at-msa.microscopy.com} ; Sun, 13 Nov 2005 17:05:58 -0600 16, 27 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 16, 27 -- by vic-ironport-ext-out3.csiro.au with ESMTP; 14 Nov 2005 10:05:57 +1100 16, 27 -- X-IronPort-AV: i="3.97,324,1125842400"; 16, 27 -- d="scan'208"; a="58144591:sNHT22819432" 16, 27 -- Received: from exvic5-gex.nexus.csiro.au ([138.194.194.6]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 16, 27 -- Mon, 14 Nov 2005 10:05:56 +1100 16, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 16, 27 -- content-class: urn:content-classes:message 16, 27 -- MIME-Version: 1.0 16, 27 -- Content-Type: text/plain; 16, 27 -- charset="us-ascii" 16, 27 -- Subject: Hitachi S4300SE/N information 16, 27 -- Date: Mon, 14 Nov 2005 10:05:55 +1100 16, 27 -- Message-ID: {32CDDDAA7161394599F0025494915749024788-at-exvic5-gex.nexus.csiro.au} 16, 27 -- X-MS-Has-Attach: 16, 27 -- X-MS-TNEF-Correlator: 16, 27 -- Thread-Topic: Hitachi S4300SE/N information 16, 27 -- Thread-Index: AcXopsz/6wlzjBoTQoaszGJ13t0mMg== 16, 27 -- From: {Colin.Veitch-at-csiro.au} 16, 27 -- To: {microscopy-at-msa.microscopy.com} 16, 27 -- X-OriginalArrivalTime: 13 Nov 2005 23:05:56.0001 (UTC) FILETIME=[CD824910:01C5E8A6] 16, 27 -- Content-Transfer-Encoding: 8bit 16, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jADN5vkk012868 ==============================End of - Headers==============================
I'm after something similar - a two axis motorised tilt plus rotate device, must tilt to at least 90 degrees and rotate 360, that can be easily and reveribly attached to the X-Y motorised stage of an SEM.
Doesn't have to be stunningly precise, does have to be cheap, and could be designed for any of the standard pin or stub mounts. cheers Sally
Dr SJ Stowe Facility Coordinator ANU Electron Microscopy Unit ANU CRICOS#00120C
} -----Original Message----- } From: gary-at-gaugler.com [mailto:gary-at-gaugler.com] } Sent: Monday, 14 November 2005 6:17 AM } To: sally.stowe-at-anu.edu.au } Subject: [Microscopy] specimen rotation unit } } } } } } --------------------------------------------------------------- } ------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
==============================Original Headers============================== 8, 26 -- From sally.stowe-at-anu.edu.au Sun Nov 13 20:05:56 2005 8, 26 -- Received: from mail.rsbs.anu.edu.au (mail.rsbs.anu.edu.au [150.203.72.70]) 8, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAE25thv023519 8, 26 -- for {microscopy-at-microscopy.com} ; Sun, 13 Nov 2005 20:05:55 -0600 8, 26 -- Received: from rsbs2262 (rsbs2262.rsbs.anu.edu.au [150.203.36.144]) 8, 26 -- by mail.rsbs.anu.edu.au (Postfix) with ESMTP 8, 26 -- id 9DA61F4406F; Mon, 14 Nov 2005 13:05:33 +1100 (EST) 8, 26 -- Reply-To: {Sally.Stowe-at-anu.edu.au} 8, 26 -- From: "Sally Stowe" {Sally.Stowe-at-anu.edu.au} 8, 26 -- To: {gary-at-gaugler.com} , {microscopy-at-microscopy.com} 8, 26 -- Subject: RE: [Microscopy] specimen rotation unit 8, 26 -- Date: Mon, 14 Nov 2005 13:05:32 +1100 8, 26 -- Message-ID: {003301c5e8bf$e6cfc1e0$9024cb96-at-rsbs.anu.edu.au} 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-Type: text/plain; 8, 26 -- charset="us-ascii" 8, 26 -- Content-Transfer-Encoding: 7bit 8, 26 -- X-Priority: 3 (Normal) 8, 26 -- X-MSMail-Priority: Normal 8, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 8, 26 -- In-Reply-To: {200511131917.jADJHPeR001753-at-ns.microscopy.com} 8, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1506 8, 26 -- Importance: Normal 8, 26 -- X-RSBS-MailScanner-Information: Please contact the ISP for more information 8, 26 -- X-RSBS-MailScanner: Found to be clean 8, 26 -- X-MailScanner-From: sally.stowe-at-anu.edu.au ==============================End of - Headers==============================
Deben UK has a tilt and rotate unit that is meant to work like you say. It is not what I am looking for but may be for you. The posted unit is for JEOL.
http://www.deben.co.uk/details.php?id=17
As a user/consumer, the Deben products are quite good.
gary g.
At 06:08 PM 11/13/2005, you wrote:
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==============================Original Headers============================== 10, 25 -- From gary-at-gaugler.com Sun Nov 13 21:05:46 2005 10, 25 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jAE35kRs000373 10, 25 -- for {microscopy-at-microscopy.com} ; Sun, 13 Nov 2005 21:05:46 -0600 10, 25 -- Received: (qmail 18265 invoked from network); 13 Nov 2005 19:05:02 -0800 10, 25 -- Received: by simscan 1.1.0 ppid: 18251, pid: 18252, t: 3.1596s 10, 25 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1095 spam: 3.0.3 10, 25 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 25 -- by qsmtp1 with SMTP; 13 Nov 2005 19:04:59 -0800 10, 25 -- Message-Id: {6.2.3.4.2.20051113190244.0295f070-at-mail.calweb.com} 10, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 10, 25 -- Date: Sun, 13 Nov 2005 19:05:46 -0800 10, 25 -- To: Sally.Stowe-at-anu.edu.au 10, 25 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 25 -- Subject: Re: [Microscopy] RE: specimen rotation unit 10, 25 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 25 -- In-Reply-To: {200511140208.jAE28vaX026791-at-ns.microscopy.com} 10, 25 -- References: {200511140208.jAE28vaX026791-at-ns.microscopy.com} 10, 25 -- Mime-Version: 1.0 10, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 10, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on 10, 25 -- qsmtp1.mc.surewest.net 10, 25 -- X-Spam-Level: 10, 25 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 10, 25 -- version=3.0.3 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ajheim-at-mail.usf.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ajheim-at-mail.usf.edu Name: A H
Organization: USF
Title-Subject: [Filtered] Force microscopy on Asylum MFP-3D
Question: Is anyone using the AR MFP-3D to perform aqueous force curves on soft or biological materials?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pk-at-aurorasiam.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, November 13, 2005 at 20:30:40 ---------------------------------------------------------------------------
Email: pk-at-aurorasiam.com Name: Per Kristian
Organization: Asssumption
Education: Graduate College
Location: Thailand
Question: Hi,
I want to get a microscope. My wish is to watch living organisms like viruses and bacteria to see how they behave.
We have a student working on a project in which they are growing bacterial cultures (pseudomonas and other species) in a hostile environment. The bacteria form capsules that we are interested in seeing. The capsules are most likely composed of sugars (polysaccharides), and we are hoping to find a fixation or other preparation technique that would help us with imaging these. We have so far prepared cultures with standard fixation, dehydration, and critical point drying techniques, but are not sure that we are preserving the capsules forming around the bacteria.
We do not have a cryo stage available.
Any ideas?
Karl Hagglund Biological Sciences, SC-102B Northern Kentucky University, Nunn Drive Highland Heights, KY 41099 859-572-5238 http://semlab.nku.edu
==============================Original Headers============================== 5, 23 -- From hagglundk1-at-nku.edu Mon Nov 14 09:24:34 2005 5, 23 -- Received: from mailfe2.nku.edu (mailfe2.nku.edu [192.122.237.68]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEFOX2R007404 5, 23 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 09:24:34 -0600 5, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211); 5, 23 -- Mon, 14 Nov 2005 10:24:33 -0500 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 5, 23 -- Content-class: urn:content-classes:message 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="us-ascii" 5, 23 -- Subject: SEM imaging of bacteria encapsulation 5, 23 -- Date: Mon, 14 Nov 2005 10:24:32 -0500 5, 23 -- Message-ID: {F01E4499C4EC5842A8AB7198141AC72F358580-at-mailfac1.hh.nku.edu} 5, 23 -- X-MS-Has-Attach: 5, 23 -- X-MS-TNEF-Correlator: 5, 23 -- Thread-Topic: SEM imaging of bacteria encapsulation 5, 23 -- Thread-Index: AcXpL4OOebjPXtKaTXWksgdATORaTw== 5, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu} 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- X-OriginalArrivalTime: 14 Nov 2005 15:24:33.0661 (UTC) FILETIME=[83F62AD0:01C5E92F] 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAEFOX2R007404 ==============================End of - Headers==============================
You might try using some Alcian blue in the primary fix to stabilise the capsules. Works for us for TEM with Campylobacter.
Chris
----- Original Message ----- X-from: {hagglundk1-at-nku.edu} To: {c.jeffree-at-ed.ac.uk} Sent: Monday, November 14, 2005 3:25 PM
There are "non-aqueous" fixation techniques and methods involving ruthenium red and alcian blue that are useful in preserving these capsules and other biofilms. The first method involves dissolving osmium tetroxide in a solvent, such as FC-72 from 3M company, rather than in an aqueous buffer.
For details, see Microscopy Research And Technique 36:390-399 (1997) and 36:422-427 (1997). Also, Biotech Histochem 66(4):173-80 (1991) and J Comp Pathology 117(2):165-70 (1997).
If you need more references, let me know. I think I have a couple more somewhere.
Good luck, Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
-----Original Message----- X-from: hagglundk1-at-nku.edu [mailto:hagglundk1-at-nku.edu] Sent: Monday, November 14, 2005 9:26 AM To: Tindall, Randy D.
We have a student working on a project in which they are growing bacterial cultures (pseudomonas and other species) in a hostile environment. The bacteria form capsules that we are interested in seeing. The capsules are most likely composed of sugars (polysaccharides), and we are hoping to find a fixation or other preparation technique that would help us with imaging these. We have so far prepared cultures with standard fixation, dehydration, and critical point drying techniques, but are not sure that we are preserving the capsules forming around the bacteria.
We do not have a cryo stage available.
Any ideas?
Karl Hagglund Biological Sciences, SC-102B Northern Kentucky University, Nunn Drive Highland Heights, KY 41099 859-572-5238 http://semlab.nku.edu
==============================Original Headers============================== 5, 23 -- From hagglundk1-at-nku.edu Mon Nov 14 09:24:34 2005 5, 23 -- Received: from mailfe2.nku.edu (mailfe2.nku.edu [192.122.237.68]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEFOX2R007404 5, 23 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 09:24:34 -0600 5, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211); 5, 23 -- Mon, 14 Nov 2005 10:24:33 -0500 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 5, 23 -- Content-class: urn:content-classes:message 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="us-ascii" 5, 23 -- Subject: SEM imaging of bacteria encapsulation 5, 23 -- Date: Mon, 14 Nov 2005 10:24:32 -0500 5, 23 -- Message-ID: {F01E4499C4EC5842A8AB7198141AC72F358580-at-mailfac1.hh.nku.edu} 5, 23 -- X-MS-Has-Attach: 5, 23 -- X-MS-TNEF-Correlator: 5, 23 -- Thread-Topic: SEM imaging of bacteria encapsulation 5, 23 -- Thread-Index: AcXpL4OOebjPXtKaTXWksgdATORaTw== 5, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu} 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- X-OriginalArrivalTime: 14 Nov 2005 15:24:33.0661 (UTC) FILETIME=[83F62AD0:01C5E92F] 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAEFOX2R007404 ==============================End of - Headers==============================
==============================Original Headers============================== 19, 24 -- From TindallR-at-missouri.edu Mon Nov 14 09:54:13 2005 19, 24 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49]) 19, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEFsC86025261 19, 24 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 09:54:13 -0600 19, 24 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 19, 24 -- Mon, 14 Nov 2005 09:54:10 -0600 19, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 24 -- Content-class: urn:content-classes:message 19, 24 -- MIME-Version: 1.0 19, 24 -- Content-Type: text/plain; 19, 24 -- charset="US-ASCII" 19, 24 -- Subject: RE: [Microscopy] SEM imaging of bacteria encapsulation 19, 24 -- Date: Mon, 14 Nov 2005 09:54:09 -0600 19, 24 -- Message-ID: {BA876152E8653240BE8572E897083EE7980550-at-UM-EMAIL09.um.umsystem.edu} 19, 24 -- X-MS-Has-Attach: 19, 24 -- X-MS-TNEF-Correlator: 19, 24 -- Thread-Topic: [Microscopy] SEM imaging of bacteria encapsulation 19, 24 -- Thread-Index: AcXpL8jOIOYDC6r4RjO8B/O8UTBpUAAAkzVw 19, 24 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 19, 24 -- To: {hagglundk1-at-nku.edu} 19, 24 -- Cc: {microscopy-at-microscopy.com} 19, 24 -- X-OriginalArrivalTime: 14 Nov 2005 15:54:10.0342 (UTC) FILETIME=[A6F22C60:01C5E933] 19, 24 -- Content-Transfer-Encoding: 8bit 19, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAEFsC86025261 ==============================End of - Headers==============================
Tannic acid, LOW molecular weight (that is appr. MW 1600), applied either in the fixative (0.05 - 0.1 -1.0 %, be sure your glutaraldehyde is of EM-grade and free of di-, polymers), or fix as you like (buffered as usual, incl. glutaraldehyde, perhaps higher concentration = {3% to } 6%), wash as usual, postfix with OsO4 (buffered, 1-2% as usual), start dehydration with 50% EtOH, and immerse then with 1% para-phenylenediamine (PPD, use with care according to MSDS) in 70% EtOH for at least 30 - 60 min, wash the specimens several times in pure 70% EtOH (to get rid of non bound PPD) and go on with dehydration/embedding as usual......
Have not tried acetone as a dehydrating agent, but I don't see a reason why aceton as a dehydrating agent should fail.....
....... There will be an interesting "difference" in morphology, as compared to "normal" fixation and dehydration.....(also, you could add a Tannic acid LOW mol. weight [TA LMW 0.05%-0.1 % hydrous solution, filtered, finest Millipore or paperfilter) preincubation staining of the ultrathin sections grids before the classical two-step staining procedure UO2Ac/Pb-citrate (TA LMW: 5 min-8 min -at-room temperature, UO2Ac[endvolume 50 ml 1% in EtOH abs+ adding a drop of acetic acid]15 min, PbCitrate [e.g. Venable&Coggeshall] 1.5 - 3 min, depending on the freshness of the Pb-solution)
Best wishes and regards,
Wolfgang Muss Salzburg, Austria
---------- Von: hagglundk1-at-nku.edu[SMTP:hagglundk1-at-nku.edu] Antwort an: hagglundk1-at-nku.edu Gesendet: Montag, 14. November 2005 16:30 An: W.Muss-at-salk.at Betreff: [Microscopy] SEM imaging of bacteria encapsulation
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We do not have a cryo stage available.
Any ideas?
Karl Hagglund Biological Sciences, SC-102B Northern Kentucky University, Nunn Drive Highland Heights, KY 41099 859-572-5238 http://semlab.nku.edu
==============================Original Headers============================== 5, 23 -- From hagglundk1-at-nku.edu Mon Nov 14 09:24:34 2005 5, 23 -- Received: from mailfe2.nku.edu (mailfe2.nku.edu [192.122.237.68]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEFOX2R007404 5, 23 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 09:24:34 -0600 5, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211); 5, 23 -- Mon, 14 Nov 2005 10:24:33 -0500 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 5, 23 -- Content-class: urn:content-classes:message 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="us-ascii" 5, 23 -- Subject: SEM imaging of bacteria encapsulation 5, 23 -- Date: Mon, 14 Nov 2005 10:24:32 -0500 5, 23 -- Message-ID: {F01E4499C4EC5842A8AB7198141AC72F358580-at-mailfac1.hh.nku.edu} 5, 23 -- X-MS-Has-Attach: 5, 23 -- X-MS-TNEF-Correlator: 5, 23 -- Thread-Topic: SEM imaging of bacteria encapsulation 5, 23 -- Thread-Index: AcXpL4OOebjPXtKaTXWksgdATORaTw== 5, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu} 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- X-OriginalArrivalTime: 14 Nov 2005 15:24:33.0661 (UTC) FILETIME=[83F62AD0:01C5E92F] 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAEFOX2R007404 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 28 -- From W.Muss-at-salk.at Mon Nov 14 10:11:53 2005 16, 28 -- Received: from Hermes.salk.at (hermes.lks.at [193.170.167.9]) 16, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEGBqmx001995 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 10:11:52 -0600 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by hermes.lks.at (Postfix) with ESMTP id B20EB5A9041; 16, 28 -- Mon, 14 Nov 2005 17:11:50 +0100 (CET) 16, 28 -- Received: from hermes.lks.at ([127.0.0.1]) 16, 28 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id 64433-06; Mon, 14 Nov 2005 17:11:50 +0100 (CET) 16, 28 -- Received: from c1pa003 (unknown [192.168.42.3]) 16, 28 -- by hermes.lks.at (Postfix) with SMTP id 6C6BA5A9046; 16, 28 -- Mon, 14 Nov 2005 17:11:50 +0100 (CET) 16, 28 -- Received: by localhost with Microsoft MAPI; Mon, 14 Nov 2005 17:11:47 +0100 16, 28 -- Message-ID: {01C5E93E.7EEE0760.W.Muss-at-salk.at} 16, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at} 16, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at} 16, 28 -- To: "'hagglundk1-at-nku.edu'" {hagglundk1-at-nku.edu} 16, 28 -- Cc: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 16, 28 -- Subject: [Microscopy] Re: SEM imaging of bacteria encapsulation 16, 28 -- Date: Mon, 14 Nov 2005 17:11:47 +0100 16, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at} 16, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor 16, 28 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211 16, 28 -- MIME-Version: 1.0 16, 28 -- Content-Type: text/plain; charset="us-ascii" 16, 28 -- Content-Transfer-Encoding: 7bit 16, 28 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at ==============================End of - Headers==============================
Saccharides are seen in very high contrast with the use of ruthenium red. It is described in some of the older literature by Luft, I think. Carol
} Date: Mon, 14 Nov 2005 09:25:52 -0600 } To: heckman-at-bgnet.bgsu.edu } From: hagglundk1-at-nku.edu } Reply-to: hagglundk1-at-nku.edu } X-Resent-From:" Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] SEM imaging of bacteria encapsulation } X-lewp: MicroscopyListSpam NAGS } X-MASF: 0.00% } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- __ Carol A. Heckman, Ph.D. Professor of Biological Sciences Director, Center for Microscopy & Microanalysis Bowling Green State University, Bowling Green, OH 43403 fax: (419) 372-2024 email: heckman-at-bgnet.bgsu.edu http://www.bgsu.edu/departments/biology/facilities/MnM ___________________________________________________________________________
==============================Original Headers============================== 4, 15 -- From heckman-at-bgnet.bgsu.edu Mon Nov 14 10:19:40 2005 4, 15 -- Received: from smtp01.bgsu.edu (smtp01.bgsu.edu [129.1.5.17]) 4, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEGJdgU010823 4, 15 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 10:19:40 -0600 4, 15 -- Received: from [129.1.85.81] (dhcp-85-81.bgsu.edu [129.1.85.81]) 4, 15 -- by smtp01.bgsu.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id jAEGJbbH003240 4, 15 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 11:19:38 -0500 (EST) 4, 15 -- Mime-Version: 1.0 4, 15 -- X-Sender: heckman-at-mailstore.bgsu.edu 4, 15 -- Message-Id: {p04320400bf9e936817a4-at-[129.1.85.81]} 4, 15 -- Date: Mon, 14 Nov 2005 11:19:39 -0800 4, 15 -- To: " Microscopy Listserver" {microscopy-at-microscopy.com} 4, 15 -- From: Carol Heckman {heckman-at-bgnet.bgsu.edu} 4, 15 -- Subject: Fwd: [Microscopy] SEM imaging of bacteria encapsulation 4, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Try your local microscope repair service. Often the have refurbished good to high quality used microscopes.
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On Nov 10, 2005, at 3:40 PM, foxglovelj-at-msn.com wrote:
} Email: foxglovelj-at-msn.com } Name: Laura Giard } } Education: Undergraduate College } } Location: Sterling, MA USA } } Title: microscope purchase } } Question: Hello, I have a 10 year old son who wants a microscope for } xmas. He wants to be a scientist when he grow up! We have purchased } "cheap" microscopes over the years and have been very disappointed. } I'd like to find one that truly works, however, cannot spend a } fortune. Can you give me some help? Magnifications etc. do not } really help me in determining the quality which is the only } information I can seem to gain from places that sell them. Right now } I've been looking at ones through Nasco. ANY HELP would be GREATLY } APPRECIATED! Thank you. } Dear Laura, Check out Project MICRO; go to the MSA web site (www.msa.microscopy.org), click on the Reference & Educational button on the left side, and then on ProjectMICRO, the 6th item under Education Committee Functions. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 23 -- From tivol-at-caltech.edu Thu Nov 10 18:32:23 2005 4, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAB0WMRW007098 4, 23 -- for {microscopy-at-msa.microscopy.com} ; Thu, 10 Nov 2005 18:32:22 -0600 4, 23 -- Received: from localhost (earth-dog [192.168.1.3]) 4, 23 -- by water-ox-postvirus (Postfix) with ESMTP 4, 23 -- id 5C330109CE1; Thu, 10 Nov 2005 16:32:20 -0800 (PST) 4, 23 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 4, 23 -- by water-ox.its.caltech.edu (Postfix) with ESMTP 4, 23 -- id 6044833BDF; Thu, 10 Nov 2005 16:32:18 -0800 (PST) 4, 23 -- In-Reply-To: {200511102340.jAANejsU020065-at-ns.microscopy.com} 4, 23 -- References: {200511102340.jAANejsU020065-at-ns.microscopy.com} 4, 23 -- Mime-Version: 1.0 (Apple Message framework v623) 4, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 23 -- Message-Id: {42d71c1996556eb227e641f0f7616792-at-caltech.edu} 4, 23 -- Content-Transfer-Encoding: 7bit 4, 23 -- Cc: microscopy-at-msa.microscopy.com 4, 23 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 23 -- Subject: Re: [Microscopy] AskAMicroscopist: 10 year old son who wants a microscope for xmas 4, 23 -- Date: Thu, 10 Nov 2005 16:36:57 -0800 4, 23 -- To: foxglovelj-at-msn.com 4, 23 -- X-Mailer: Apple Mail (2.623) 4, 23 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.3.2 ==============================End of - Headers==============================
==============================Original Headers============================== 6, 17 -- From DFORAN-at-ORA.FDA.GOV Mon Nov 14 10:20:11 2005 6, 17 -- Received: from wall3-pub.fda.gov (wall3-pub.fda.gov [150.148.0.65]) 6, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEGKBkY012154 6, 17 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 10:20:11 -0600 6, 17 -- Received: from orshq08a.fda.gov by wall3-pub.fda.gov 6, 17 -- via smtpd (for microscopy.com [206.69.208.10]) with ESMTP; Mon, 14 Nov 2005 11:20:11 -0500 6, 17 -- Received: by orshq08a.fda.gov with Internet Mail Service (5.5.2657.72) 6, 17 -- id {46RXWSSX} ; Mon, 14 Nov 2005 11:20:10 -0500 6, 17 -- Message-ID: {D117D2B78C6BA24C9500F2431F6F260A024C6254-at-orsswkc02.fda.gov} 6, 17 -- From: "Foran, David A" {DFORAN-at-ORA.FDA.GOV} 6, 17 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 6, 17 -- Subject: FW: [Microscopy] Re: AskAMicroscopist: 10 year old son who wants 6, 17 -- a microscope for xmas 6, 17 -- Date: Mon, 14 Nov 2005 11:19:41 -0500 6, 17 -- MIME-Version: 1.0 6, 17 -- X-Mailer: Internet Mail Service (5.5.2657.72) 6, 17 -- Content-Type: text/plain ==============================End of - Headers==============================
Freeze drying might be the best bet for holding on the everything without adding artefactual material such as Ru.
hagglundk1-at-nku.edu wrote:
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-- Gregory W. Erdos, Ph.D, Assistant Director, Biotechnology Program Scientific Director, EM Core Lab P.O. Box 118525 University of Florida Gainesville, FL 32611 gwe-at-ufl.edu Phone: 352-392-1295 Fax: 352-846-0251
==============================Original Headers============================== 5, 24 -- From gwe-at-ufl.edu Mon Nov 14 10:46:08 2005 5, 24 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 5, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEGk76g028740 5, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 14 Nov 2005 10:46:07 -0600 5, 24 -- Received: from [128.227.60.168] (empc14419.dhcp.clas.ufl.edu [128.227.60.168]) 5, 24 -- by smtp.ufl.edu (8.13.4/8.13.4/2.5.1) with ESMTP id jAEGk5hi1163340; 5, 24 -- Mon, 14 Nov 2005 11:46:06 -0500 5, 24 -- Message-ID: {4378BF4F.8050805-at-ufl.edu} 5, 24 -- Date: Mon, 14 Nov 2005 11:46:07 -0500 5, 24 -- From: Greg Erdos {gwe-at-ufl.edu} 5, 24 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 5, 24 -- X-Accept-Language: en-us, en 5, 24 -- MIME-Version: 1.0 5, 24 -- To: hagglundk1-at-nku.edu, 5, 24 -- "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com} 5, 24 -- Subject: Re: [Microscopy] SEM imaging of bacteria encapsulation 5, 24 -- References: {200511141525.jAEFPfpw008717-at-ns.microscopy.com} 5, 24 -- In-Reply-To: {200511141525.jAEFPfpw008717-at-ns.microscopy.com} 5, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- X-Spam-Status: hits=-0.001, required=5, tests=BAYES_44 5, 24 -- X-UFL-Spam-Status: hits=-0.001, required=5, tests=BAYES_44 5, 24 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 5, 24 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
We are in the process to get a CCD camera for our TEM. I am not very familiar with the market. Does anyone have any suggestion in terms of which companies are reliable and offer good customer service all that. I remember there was a user gathering a few years ago at MSA meeting to discuss problems with one particular company. I was not at that gathering so did not know a lot of details. Can anyone tell me what are the issues when buying a CCD camera?
Hope to hear your opinions. Thank you in advance.
Hong Emory EM
==============================Original Headers============================== 6, 22 -- From hyi-at-emory.edu Mon Nov 14 11:56:33 2005 6, 22 -- Received: from nemausa.cc.emory.edu (nemausa.cc.emory.edu [170.140.8.220]) 6, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEHuWWH006041 6, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 11:56:32 -0600 6, 22 -- Received: from [170.140.233.138] (localhost [127.0.0.1]) 6, 22 -- by nemausa.cc.emory.edu (8.13.4/8.13.4) with ESMTP id jAEHuTcN029659 6, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 12:56:29 -0500 (EST) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v622) 6, 22 -- Message-Id: {92f39a75dfb00e1ef962bd53630aa3bb-at-emory.edu} 6, 22 -- Content-Type: text/plain; 6, 22 -- charset=ISO-8859-1; 6, 22 -- format=flowed 6, 22 -- To: Microscopy-at-microscopy.com 6, 22 -- From: Hong Yi {hyi-at-emory.edu} 6, 22 -- Subject: CCD camera for TEM 6, 22 -- Date: Mon, 14 Nov 2005 12:56:26 -0500 6, 22 -- X-Mailer: Apple Mail (2.622) 6, 22 -- X-imss-version: 2.034 6, 22 -- X-imss-result: Passed 6, 22 -- X-imss-approveListMatch: *-at-emory.edu 6, 22 -- Content-Transfer-Encoding: 8bit 6, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAEHuWWH006041 ==============================End of - Headers==============================
On Nov 14, 2005, at 7:24 AM, hagglundk1-at-nku.edu wrote:
} We have a student working on a project in which they are growing } bacterial cultures (pseudomonas and other species) in a hostile } environment. The bacteria form capsules that we are interested in } seeing. The capsules are most likely composed of sugars } (polysaccharides), and we are hoping to find a fixation or other } preparation technique that would help us with imaging these. We have } so } far prepared cultures with standard fixation, dehydration, and critical } point drying techniques, but are not sure that we are preserving the } capsules forming around the bacteria. } } We do not have a cryo stage available. } Dear Karl, Two possibilities are negative stain and cryofixation. Even though you do not have a cryostage, high-pressure freezing followed by freeze-substitution and embedding will give you the specimen in a block of resin that can be stained and sectioned in the conventional manner. Of course, you may not have access to a high-pressure freezer. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Mon Nov 14 12:41:54 2005 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEIfrTg015284 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 14 Nov 2005 12:41:53 -0600 4, 22 -- Received: from localhost (fire-dog [192.168.1.4]) 4, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 93222352BF 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 14 Nov 2005 10:41:51 -0800 (PST) 4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 4, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id E9C59352E8 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 14 Nov 2005 10:41:50 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v623) 4, 22 -- In-Reply-To: {200511141524.jAEFOkLt007583-at-ns.microscopy.com} 4, 22 -- References: {200511141524.jAEFOkLt007583-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {090ef66e30ba63a29ca02e2df2b984fc-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] SEM imaging of bacteria encapsulation 4, 22 -- Date: Mon, 14 Nov 2005 10:46:38 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.623) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.3.2 ==============================End of - Headers==============================
You might like to try a simplified cryo-fixation method. It is not the best method for many reasons and may not give you an impressive result as the high-pressure freezer that Bill Tivol suggests, but it will give you an easy way into these methods. Hopefully the results will give you enough data for a grant!
First freeze your bacteria by immersion freezing in as close to the growing state as possible. If they are growing in suspension, spin them down very gently and scoop out the pellet to place onto a metal holder (any small piece of wire or metal that can hold a drop of the suspension will work). If they are growing on a substrate, leave them there.
Take the bacteria and freeze them by immersion in an efficient cryogen. We use liquid propane from a bottle we get from a camping store (for cooking food outdoors) - disclaimer here: propane is inflammable so take suitable precautions not to blow up the lab. We collect the propane by piping it into a small plastic cup that is surrounded by liquid nitrogen.
Once the bacteria have been frozen, which is almost immediately after they have been immersed in the propane, transfer them to vials on dry ice, containing dry ethanol or acetone. The dry ice is held in a styrofoam box Leave them on the dry ice for a few days (depending on the size of the specimen), changing the cold solvent with fresh cold solvent every day.
Transfer the specimens to the fridge (4 degrees) in a small styrofoam box containing a small amount of dry ice and leave the specimens to warm to 4 degrees. The trick is to let the vials warm up slowly enough to not have the solvent boil.
Once you have the solvent at 4 degrees, the specimens can be transferred to a critical point drier and processed for SEM examination.
As I said, this is not the best way to prepare specimens using cryomethods but you may be surprised by the results you get.
Paul Webster.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
On 11/14/05 7:30 AM, "hagglundk1-at-nku.edu" {hagglundk1-at-nku.edu} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We have a student working on a project in which they are growing } bacterial cultures (pseudomonas and other species) in a hostile } environment. The bacteria form capsules that we are interested in } seeing. The capsules are most likely composed of sugars } (polysaccharides), and we are hoping to find a fixation or other } preparation technique that would help us with imaging these. We have so } far prepared cultures with standard fixation, dehydration, and critical } point drying techniques, but are not sure that we are preserving the } capsules forming around the bacteria. } } We do not have a cryo stage available. } } Any ideas? } } Karl Hagglund } Biological Sciences, SC-102B } Northern Kentucky University, Nunn Drive } Highland Heights, KY 41099 } 859-572-5238 } http://semlab.nku.edu } } } ==============================Original Headers============================== } 5, 23 -- From hagglundk1-at-nku.edu Mon Nov 14 09:24:34 2005 } 5, 23 -- Received: from mailfe2.nku.edu (mailfe2.nku.edu [192.122.237.68]) } 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEFOX2R007404 } 5, 23 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 09:24:34 -0600 } 5, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu } with Microsoft SMTPSVC(6.0.3790.211); } 5, 23 -- Mon, 14 Nov 2005 10:24:33 -0500 } 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 } 5, 23 -- Content-class: urn:content-classes:message } 5, 23 -- MIME-Version: 1.0 } 5, 23 -- Content-Type: text/plain; } 5, 23 -- charset="us-ascii" } 5, 23 -- Subject: SEM imaging of bacteria encapsulation } 5, 23 -- Date: Mon, 14 Nov 2005 10:24:32 -0500 } 5, 23 -- Message-ID: } {F01E4499C4EC5842A8AB7198141AC72F358580-at-mailfac1.hh.nku.edu} } 5, 23 -- X-MS-Has-Attach: } 5, 23 -- X-MS-TNEF-Correlator: } 5, 23 -- Thread-Topic: SEM imaging of bacteria encapsulation } 5, 23 -- Thread-Index: AcXpL4OOebjPXtKaTXWksgdATORaTw== } 5, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu} } 5, 23 -- To: {microscopy-at-microscopy.com} } 5, 23 -- X-OriginalArrivalTime: 14 Nov 2005 15:24:33.0661 (UTC) } FILETIME=[83F62AD0:01C5E92F] } 5, 23 -- Content-Transfer-Encoding: 8bit } 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id jAEFOX2R007404 } ==============================End of - Headers==============================
==============================Original Headers============================== 20, 20 -- From PWebster-at-hei.org Mon Nov 14 12:58:26 2005 20, 20 -- Received: from hi0sml1.hei.org (House-Ear-Institute-1114861.cust-rtr.pacbell.net [71.136.31.242]) 20, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEIwQLs024351 20, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 12:58:26 -0600 20, 20 -- Received: from 10.10.42.113 ([10.10.42.113]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 20, 20 -- Mon, 14 Nov 2005 18:57:52 +0000 20, 20 -- User-Agent: Microsoft-Entourage/11.2.1.051004 20, 20 -- Date: Mon, 14 Nov 2005 10:53:19 -0800 20, 20 -- Subject: Re: [Microscopy] SEM imaging of bacteria encapsulation 20, 20 -- From: "Webster, Paul" {PWebster-at-hei.org} 20, 20 -- To: {hagglundk1-at-nku.edu} 20, 20 -- CC: {Microscopy-at-microscopy.com} 20, 20 -- Message-ID: {BF9E1D1F.659E%PWebster-at-hei.org} 20, 20 -- Thread-Topic: [Microscopy] SEM imaging of bacteria encapsulation 20, 20 -- Thread-Index: AcXpTK2l7HAk5FU/Edq7aAANk7Zh7g== 20, 20 -- In-Reply-To: {200511141530.jAEFUg9s015368-at-ns.microscopy.com} 20, 20 -- Mime-version: 1.0 20, 20 -- Content-type: text/plain; 20, 20 -- charset="US-ASCII" 20, 20 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
} } Title: microscope purchase } } } } Question: Hello, I have a 10 year old son who wants a microscope for } } xmas. He wants to be a scientist when he grow up! We have purchased } } "cheap" microscopes over the years and have been very disappointed. } } I'd like to find one that truly works, however, cannot spend a } } fortune. Can you give me some help? Magnifications etc. do not } } really help me in determining the quality which is the only } } information I can seem to gain from places that sell them. Right now } } I've been looking at ones through Nasco. ANY HELP would be GREATLY } } APPRECIATED! Thank you. } } } Dear Laura,
I have a page that I put together for the first time buyer of microscopes http://www.couger.com/microscope/links/gcnewbuy.html
While I am normally very strongly in favor of buying older used microscopes in the case of young children it is almost impossible to find what they need.
If you buy used buy from a dealer if you buy on Ebay research the sellers feedback and other sales very carefully. There are some good dealers on ebay but you have to do you home work.
Gordon.
========= From My Page === Special Consideration for Children.
Microscopes for children present special considerations. First it is important that the microscope be an instrument that they use and not play with a few days and discard. In these days of the Internet and instant gratification holding my interested is difficult holding a a child's is a real challenge. Getting a scope too complex for them to use on their own is a sure way to put most kids off. I know it did me over 50 years ago and many complex procedures still do.
Another problem is young children's eyes are closer together than most binocular tubes will close up and their binocular vision not as well developed so binocular heads may pose a problem for them well into their teens. Even if the scope will close down enough to accommodate their eyes the muscular coordination of their eyes may not work with the binocular head pieces. Having multiple sclerosis I have experienced this and it quickly causes eyestrain if the eyes do not comfortably lock in on the image.
Of course having eyepieces and other parts that are attached so that they are difficult to remove and lose or injure the child are important.
It is difficult to find this qualities in a used microscopes so you are left with little choice but to buy from a dealer in new microscopes. I hesitate to recommend particular dealer. I don't hesitate to strongly recommend that you by from someone that has been a microscope dealer for some years and has a good reputation for marinating the equipment they sell. Buying from retail stores that have no microscope service department is no different than buying from ebay except it is easy to find the person to complain to when things don't work. It is not much easier to get them fixed in most cases.
For their fist scope a low power 10 to 30 power scope that requires little or no surface preparation may well hold their interest much longer than a height power compound scope that require specimen preparation, careful lighting and scope adjustment to get a decent image. All this is speaking in generalities there are 6 year old kids that can master a complex scope just as there are grown men than can't work a magnifying glass.
While I don't recommend any particular seller I use this scope as an example of a good low power scope for the beginner http://www.microscopeworld.com/low/lpdis.htm it is a 20x scope that has all pieces attached so they aren't remove by curious hands and the monocular set up allow easy use with out constant readjustment among users and children that have problems with binocular vision have no problems.
Scopes like these http://www.microscopeworld.com/high/hpin.htm are very good choice for youngsters. Get one with a simple condenser. I allows you to work with them and the light and condenser actual adjustable stops are simple enough children can use them unaided. Less expensive used scopes can be purchased with better lenses but you will be hard pressed to find a simpler scope than this one. Defused light and simple stop system is more that adequate and much less frustrating than an adjustable condenser and iris 40x and lower powered objectives. The contrast suffers a little at 40x but it is still good enough for very good viewing. Actual test surprised me.
If the child has a real interest in science I would let him try some more complex scopes and see what he can handle and try to come to a realistic conclusion of his desires, abilities and drive to complete projects before getting a complex microscope.
If you are considering a microscope for a gift to a child consider the level of complexity the child can deal with. Condensers can give them a hard time so get one with a simple condenser system or be prepared to spend a lot of time with them if you get a complex one. Also binocular heads are a problem. They may not close to the point that they can use them and until their middle teens there binocular vision may not develop to be able to use them very well.
In used compound scopes I have used the AO Spencer 160 with very good results.
For the younger child I have found nothing better than the this Asian import. http://www.microscopeworld.com/low/lpdis.htm
Occasionally a monocular dissection scope comes up on ebay but not often. These scopes take little or no preparation of specials and are less likely to end up in the closet if the child finds things to complex. For a binocular scope AO Spencer Cycloptics sell for $ 50 and up on ebay and www.roseoptics.com has some serviced and rebuilt ones that have the prisms attached with modern cement that he sells for a reasonable price with a guarantee. These old Cycloptics are ideal for children because they are so sturdy and almost any one can clean sand dirt out of them if they children get careless. and they offer 7 to 25x or 14 to 50x with a 2x lens.
==============================Original Headers============================== 23, 21 -- From gcc-at-couger.com Mon Nov 14 13:56:13 2005 23, 21 -- Received: from centrmmtao03.cox.net (centrmmtao03.cox.net [70.168.83.81]) 23, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEJuDcI001695 23, 21 -- for {microscopy-at-msa.microscopy.com} ; Mon, 14 Nov 2005 13:56:13 -0600 23, 21 -- Received: from [127.0.0.1] (really [68.12.242.13]) by centrmmtao03.cox.net 23, 21 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 23, 21 -- id {20051114195456.BUIO21645.centrmmtao03.cox.net-at-[127.0.0.1]} ; 23, 21 -- Mon, 14 Nov 2005 14:54:56 -0500 23, 21 -- Message-ID: {4378EBE4.5030903-at-couger.com} 23, 21 -- Date: Mon, 14 Nov 2005 13:56:20 -0600 23, 21 -- From: Gordon Couger {gcc-at-couger.com} 23, 21 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 23, 21 -- X-Accept-Language: en-us, en 23, 21 -- MIME-Version: 1.0 23, 21 -- To: DFORAN-at-ORA.FDA.GOV, 23, 21 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} 23, 21 -- Subject: Re: [Microscopy] FW: Re: AskAMicroscopist: 10 year old son who wants 23, 21 -- References: {200511141623.jAEGN2Hj021191-at-ns.microscopy.com} 23, 21 -- In-Reply-To: {200511141623.jAEGN2Hj021191-at-ns.microscopy.com} 23, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 23, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I was wondering how other listers store their TEM samples between preparation and microscope time. We have oxidation problems, and I am curious whether it is better to store samples under vacuum or an inert gas, as far as preventing/delaying oxidation. Right now we store ours in a cabinet pumped out by a diaphragm pump, and we see contamination even after a couple days on our sensitive samples.
Thank you, Leslie
Leslie Krupp (Thompson) IBM Almaden Research 650 Harry Road, K19/D2 San Jose, CA 95120-6099
==============================Original Headers============================== 4, 27 -- From lkrupp-at-us.ibm.com Mon Nov 14 14:11:36 2005 4, 27 -- Received: from e3.ny.us.ibm.com (e3.ny.us.ibm.com [32.97.182.143]) 4, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEKBaEF010687 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 14:11:36 -0600 4, 27 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236]) 4, 27 -- by e3.ny.us.ibm.com (8.12.11/8.12.11) with ESMTP id jAEKBMEY012768 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 15:11:22 -0500 4, 27 -- Received: from d01av03.pok.ibm.com (d01av03.pok.ibm.com [9.56.224.217]) 4, 27 -- by d01relay04.pok.ibm.com (8.12.10/NCO/VERS6.8) with ESMTP id jAEKBM4S103500 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 15:11:22 -0500 4, 27 -- Received: from d01av03.pok.ibm.com (loopback [127.0.0.1]) 4, 27 -- by d01av03.pok.ibm.com (8.12.11/8.13.3) with ESMTP id jAEKBLst003418 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 15:11:22 -0500 4, 27 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) 4, 27 -- by d01av03.pok.ibm.com (8.12.11/8.12.11) with ESMTP id jAEKBLSV003412 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 15:11:21 -0500 4, 27 -- To: microscopy-at-microscopy.com 4, 27 -- MIME-Version: 1.0 4, 27 -- Subject: TEM sample storage 4, 27 -- X-Mailer: Lotus Notes Release 6.0.2CF1 June 9, 2003 4, 27 -- Message-ID: {OFBDA21EB5.E7D10289-ON852570B9.006DADF5-882570B9.006EE592-at-us.ibm.com} 4, 27 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} 4, 27 -- Date: Mon, 14 Nov 2005 12:11:16 -0800 4, 27 -- X-MIMETrack: Serialize by Router on D01ML604/01/M/IBM(Release 7.0HF60 | October 19, 2005) at 4, 27 -- 11/14/2005 15:11:21, 4, 27 -- Serialize complete at 11/14/2005 15:11:21 4, 27 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
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Email: m.obrien-at-sgul.ac.uk Name: Marc O'Brien
Organization: St George's University of London
Title-Subject: [Filtered] help with Colorview12 camera and GrabBit card.
Question: Hi
We have an Olympus BX51 with a Soft Imaging Systems framegrabber camera and software, we've tried upgrading the PC but have found that PCI GrabBit card is not recognised by the motherboard. SIS say they can upgrade the card for around 800 euros, but can't supply a loan card while the GrabBit is away being upgraded. They also state that the colorview 12 camera will not work with any other framegrabber card .
We are loathe to dismantle our only working system.
Our only option would seem to be buying or borrowing a spare PCI grabBit, and then having the upgrade done, thus keeping a working system.
Does anyone have one of these cards spare?
Does anyone know of other framegrabber cards that work with the colorview12 camera and Analysis 5 software?
Has anyone been through this process with SIS and have any comments.
Any other thoughts or comments gratefully received.
We've had some success in preserving easily oxidized samples by using a rotary vane vacuum pump, grade 5 argon, and a vacuum desiccator. We just put the samples in the desiccator (with or without desiccant - your choice for your application), then triple purge it with vacuum & argon, ultimately leaving it under active vacuum. When ready for the samples, back-filling with the argon again will buy you enough time to open up the desiccator and remove your sample. We've seen a definite difference between the use of nitrogen and the use of argon with argon being better at displacing the oxygen in the system.
Hope that helps, John
} Hello- } } I was wondering how other listers store their TEM samples between } preparation and microscope time. We have oxidation problems, and I am } curious whether it is better to store samples under vacuum or an inert } gas, as far as preventing/delaying oxidation. Right now we store ours in } a cabinet pumped out by a diaphragm pump, and we see contamination even } after a couple days on our sensitive samples. } } Thank you, } Leslie } } Leslie Krupp (Thompson) } IBM Almaden Research } 650 Harry Road, K19/D2 } San Jose, CA 95120-6099
John Papalia Galvin Research Group 201 duPont Hall Dept. of Materials Science & Engineering University of Delaware Newark, DE 19716
==============================Original Headers============================== 7, 19 -- From papalia-at-udel.edu Mon Nov 14 15:14:52 2005 7, 19 -- Received: from md1.nss.udel.edu (md1.nss.udel.edu [128.175.1.11]) 7, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAELEpvV000386 7, 19 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 15:14:51 -0600 7, 19 -- Received: from denali.udel.edu (host-228-184.nss.udel.edu [128.175.228.184]) 7, 19 -- by md1.nss.udel.edu (MOS 3.7.1-GA) 7, 19 -- with ESMTP id AYI44237 (AUTH via LOGINBEFORESMTP); 7, 19 -- Mon, 14 Nov 2005 16:10:47 -0500 (EST) 7, 19 -- Message-Id: {6.2.3.4.0.20051114155642.042f2ea8-at-mail.udel.edu} 7, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 7, 19 -- Date: Mon, 14 Nov 2005 16:07:07 -0500 7, 19 -- To: lkrupp-at-us.ibm.com 7, 19 -- From: John Papalia {papalia-at-udel.edu} 7, 19 -- Subject: Re: [Microscopy] TEM sample storage 7, 19 -- Cc: microscopy-at-microscopy.com 7, 19 -- In-Reply-To: {200511142015.jAEKFJl7018085-at-ns.microscopy.com} 7, 19 -- References: {200511142015.jAEKFJl7018085-at-ns.microscopy.com} 7, 19 -- Mime-Version: 1.0 7, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
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Per - You're being a bit too ambitious; start with larger organisms. No one looks at living viruses; they're so small that an electron microscope is needed. Dead & stained bacteria can be seen with a compound microscope. If you want some fun & action, start with protozoa in pond water. Look at the MICRO bibliography & order a book or two. "Explore the world using protozoa" &/or "Guide to microlife", and "Exploring with the microscope". Start with one of the many Chinese scopes, perhaps with LED illumination. Get one that you can add an 100x oil immersion objective to, AFTER you learn how to use it. Then you'll be able to see bacteria, but they aren't going to be very exciting to watch. -- Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 2, 18 -- From schooley-at-mcn.org Mon Nov 14 19:37:27 2005 2, 18 -- Received: from dns4.mcn.org (dns4.mcn.org [216.150.240.31]) 2, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAF1bQXD018541 2, 18 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 14 Nov 2005 19:37:27 -0600 2, 18 -- Received: from [66.52.139.167] (helo=[10.0.1.2]) 2, 18 -- by dns4.mcn.org with esmtpa (Exim 4.43) 2, 18 -- id IPZ36C-00088U-II; Mon, 14 Nov 2005 17:37:25 -0800 2, 18 -- Mime-Version: 1.0 2, 18 -- Message-Id: {a06200705bf9ee8d5fb81-at-[10.0.1.2]} 2, 18 -- In-Reply-To: {200511140522.jAE5M9Xc027924-at-ns.microscopy.com} 2, 18 -- References: {200511140522.jAE5M9Xc027924-at-ns.microscopy.com} 2, 18 -- Date: Mon, 14 Nov 2005 17:39:33 -0800 2, 18 -- To: pk-at-aurorasiam.com 2, 18 -- From: Caroline Schooley {schooley-at-mcn.org} 2, 18 -- Subject: Re: [Microscopy] AskAMicroscopist: What kind of microscope should 2, 18 -- i get? 2, 18 -- Cc: Microscopy-at-MSA.Microscopy.Com 2, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Can anyone please tell me what the latest issue of The Microanalysis Suite from Oxford Instruments is currently available? What issue are you using? We are on issue 13. Our service contract entitles us for free software upgrades, but we just can not get the information from the local Oxford representatives.
Many thanks, Alexander Titkov
Millennium Inorganic Chemicals Ltd A Lyondell Company
Lyondell Chemical Company
The information contained in this e-mail message and any attachments may be confidential. It is intended only for the use of the individual or entities named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail at the originating address.
==============================Original Headers============================== 11, 22 -- From alex.titkov-at-millenniumchem.com Tue Nov 15 00:55:20 2005 11, 22 -- Received: from edcpap01.lyo.com (mail3.lyondell.com [161.16.0.77]) 11, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAF6tIx4030890 11, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 00:55:19 -0600 11, 22 -- Received: from edcexp02.lyondell.com ([161.16.33.40]) 11, 22 -- by edcpap01.lyo.com (8.13.3/8.13.1) with ESMTP id jAF6t9pG021774 11, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 22:55:14 -0800 11, 22 -- Received: from amnhhtv01.millenniumchem.com ([172.19.0.46]) by edcexp02.lyondell.com with Microsoft SMTPSVC(5.0.2195.6713); 11, 22 -- Tue, 15 Nov 2005 00:55:11 -0600 11, 22 -- Subject: INCA Energy software 11, 22 -- To: Microscopy-at-microscopy.com 11, 22 -- X-Mailer: Lotus Notes Release 5.0.11 July 24, 2002 11, 22 -- Message-ID: {OFFCE16D02.CAC8F090-ON482570BA.0023C75C-482570BA.002601C0-at-millenniumchem.com} 11, 22 -- From: alex.titkov-at-millenniumchem.com 11, 22 -- Date: Tue, 15 Nov 2005 14:55:08 +0800 11, 22 -- X-MIMETrack: Serialize by Router on AMNHHTV01/HUB/SRV/MIC(Release 5.0.11 |July 24, 2002) at 11, 22 -- 11/15/2005 01:56:14 AM 11, 22 -- MIME-Version: 1.0 11, 22 -- Content-type: text/plain; charset=us-ascii 11, 22 -- X-OriginalArrivalTime: 15 Nov 2005 06:55:11.0765 (UTC) FILETIME=[8610AC50:01C5E9B1] 11, 22 -- X-Proofpoint-Spam-Details: rule=notspam policy= score=0 mlx=0 adultscore=0 adjust=0 engine=2.5.0-05110701 definitions=3.0.0-05111403 11, 22 -- X-Proofpoint-OriginalSender: alex.titkov-at-millenniumchem.com ==============================End of - Headers==============================
Sent: 15 November 2005 07:04 To: ron.doole-at-materials.ox.ac.uk
Hi,
Can anyone please tell me what the latest issue of The Microanalysis Suite from Oxford Instruments is currently available? What issue are you using? We are on issue 13. Our service contract entitles us for free software upgrades, but we just can not get the information from the local Oxford representatives.
Many thanks, Alexander Titkov
Millennium Inorganic Chemicals Ltd A Lyondell Company
Lyondell Chemical Company
The information contained in this e-mail message and any attachments may be confidential. It is intended only for the use of the individual or entities named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail at the originating address.
==============================Original Headers============================== 11, 22 -- From alex.titkov-at-millenniumchem.com Tue Nov 15 00:55:20 2005 11, 22 -- Received: from edcpap01.lyo.com (mail3.lyondell.com [161.16.0.77]) 11, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAF6tIx4030890 11, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 00:55:19 -0600 11, 22 -- Received: from edcexp02.lyondell.com ([161.16.33.40]) 11, 22 -- by edcpap01.lyo.com (8.13.3/8.13.1) with ESMTP id jAF6t9pG021774 11, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 22:55:14 -0800 11, 22 -- Received: from amnhhtv01.millenniumchem.com ([172.19.0.46]) by edcexp02.lyondell.com with Microsoft SMTPSVC(5.0.2195.6713); 11, 22 -- Tue, 15 Nov 2005 00:55:11 -0600 11, 22 -- Subject: INCA Energy software 11, 22 -- To: Microscopy-at-microscopy.com 11, 22 -- X-Mailer: Lotus Notes Release 5.0.11 July 24, 2002 11, 22 -- Message-ID: {OFFCE16D02.CAC8F090-ON482570BA.0023C75C-482570BA.002601C0-at-millenniumchem.co m} 11, 22 -- From: alex.titkov-at-millenniumchem.com 11, 22 -- Date: Tue, 15 Nov 2005 14:55:08 +0800 11, 22 -- X-MIMETrack: Serialize by Router on AMNHHTV01/HUB/SRV/MIC(Release 5.0.11 |July 24, 2002) at 11, 22 -- 11/15/2005 01:56:14 AM 11, 22 -- MIME-Version: 1.0 11, 22 -- Content-type: text/plain; charset=us-ascii 11, 22 -- X-OriginalArrivalTime: 15 Nov 2005 06:55:11.0765 (UTC) FILETIME=[8610AC50:01C5E9B1] 11, 22 -- X-Proofpoint-Spam-Details: rule=notspam policy= score=0 mlx=0 adultscore=0 adjust=0 engine=2.5.0-05110701 definitions=3.0.0-05111403 11, 22 -- X-Proofpoint-OriginalSender: alex.titkov-at-millenniumchem.com ==============================End of - Headers==============================
==============================Original Headers============================== 23, 24 -- From ron.doole-at-materials.ox.ac.uk Tue Nov 15 02:14:08 2005 23, 24 -- Received: from relay1.mail.ox.ac.uk (relay1.mail.ox.ac.uk [129.67.1.165]) 23, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAF8E7cV008411 23, 24 -- for {microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 02:14:07 -0600 23, 24 -- Received: from smtp2.herald.ox.ac.uk ([163.1.0.235]) 23, 24 -- by relay1.mail.ox.ac.uk with esmtp (Exim 4.52) 23, 24 -- id 1Ebvx8-0001di-5y; Tue, 15 Nov 2005 08:14:06 +0000 23, 24 -- Received: from oums-doole.materials.ox.ac.uk ([129.67.85.58] helo=oumsdoole) 23, 24 -- by smtp2.herald.ox.ac.uk with esmtp (Exim 3.36 #1) 23, 24 -- id 1Ebvx8-0002l9-02; Tue, 15 Nov 2005 08:14:06 +0000 23, 24 -- From: "Ron Doole" {ron.doole-at-materials.ox.ac.uk} 23, 24 -- To: {alex.titkov-at-millenniumchem.com} 23, 24 -- Cc: {microscopy-at-microscopy.com} 23, 24 -- Subject: RE: [Microscopy] INCA Energy software 23, 24 -- Date: Tue, 15 Nov 2005 08:14:05 -0000 23, 24 -- MIME-Version: 1.0 23, 24 -- Content-Type: text/plain; 23, 24 -- charset="us-ascii" 23, 24 -- Content-Transfer-Encoding: 7bit 23, 24 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353 23, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2527 23, 24 -- Thread-Index: AcXpsrT/mmGR4nL7SSOpF8n0mhYVrwACS3Ig 23, 24 -- In-Reply-To: {200511150703.jAF73axd006875-at-ns.microscopy.com} 23, 24 -- Message-Id: {E1Ebvx8-0002l9-02-at-smtp2.herald.ox.ac.uk} ==============================End of - Headers==============================
I have a GrabBit PCI card you can borrow. $20 and a promise to return it. You pay for shipping out and back. $995 if you want to keep it.
gary g.
At 01:04 PM 11/14/2005, you wrote:
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==============================Original Headers============================== 10, 25 -- From gary-at-gaugler.com Tue Nov 15 02:16:02 2005 10, 25 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jAF8G1q2011637 10, 25 -- for {microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 02:16:02 -0600 10, 25 -- Received: (qmail 20743 invoked from network); 15 Nov 2005 00:15:17 -0800 10, 25 -- Received: by simscan 1.1.0 ppid: 20733, pid: 20734, t: 3.9075s 10, 25 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1142 spam: 3.0.3 10, 25 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 25 -- by qsmtp2 with SMTP; 15 Nov 2005 00:15:13 -0800 10, 25 -- Message-Id: {6.2.3.4.2.20051115001240.028bdf78-at-mail.calweb.com} 10, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 10, 25 -- Date: Tue, 15 Nov 2005 00:16:02 -0800 10, 25 -- To: m.obrien-at-sgul.ac.uk 10, 25 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 25 -- Subject: Re: [Microscopy] viaWWW: help with Colorview12 camera and 10, 25 -- GrabBit card. 10, 25 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 25 -- In-Reply-To: {200511142104.jAEL4gw8023694-at-ns.microscopy.com} 10, 25 -- References: {200511142104.jAEL4gw8023694-at-ns.microscopy.com} 10, 25 -- Mime-Version: 1.0 10, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 10, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp2.surewest.net 10, 25 -- X-Spam-Level: 10, 25 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 10, 25 -- version=3.0.3 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kunli218-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kunli218-at-yahoo.com Name: Simon Lee
Organization: CharteredSEMi
Title-Subject: [Filtered] MListserver:Plan view TEM sample prep contamination
Question: Dear Listers,
We met some contamination problem when preparing plan-view TEM sample where the sample can only be milled from one side. We found that the contamination at the unmilled side is quite significant (redeposition). The miller we use is PIPS from Gatan. Will the graphite post help? and Is there any other procedure to minimize the contamination?
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Email: U.J.Potter-at-bath.ac.uk Name: Ursula Potter
We have a problem with the sectioning of Zebrafish embryos (early stages with yolk sac). The embryos have been subjected to an in situ hybridization staining method and when subsequently embedded in wax or resin (technovit) the sections show very bad chatter (wax) or tearing (resin) at the area of the yolk sac. Has anyone experienced this problem? Advice to solve problem would be gratefully received.
I just received Issue 16 last week as a service contract upgrade. We've always gotten prompt responses on software and other issues from our local service and sales reps and from the MA office, with additional support from the UK if necessary. Getting the upgrade with your service contract renewal should be straightforward.
Regards,
Elaine
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
-----Original Message----- X-from: alex.titkov-at-millenniumchem.com [mailto:alex.titkov-at-millenniumchem.com] Sent: Tuesday, November 15, 2005 12:56 AM To: Elaine F. Schumacher
Hi,
Can anyone please tell me what the latest issue of The Microanalysis Suite from Oxford Instruments is currently available? What issue are you using? We are on issue 13. Our service contract entitles us for free software upgrades, but we just can not get the information from the local Oxford representatives.
Many thanks, Alexander Titkov
Millennium Inorganic Chemicals Ltd A Lyondell Company
Lyondell Chemical Company
The information contained in this e-mail message and any attachments may be confidential. It is intended only for the use of the individual or entities named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail at the originating address.
==============================Original Headers============================== 11, 22 -- From alex.titkov-at-millenniumchem.com Tue Nov 15 00:55:20 2005 11, 22 -- Received: from edcpap01.lyo.com (mail3.lyondell.com [161.16.0.77]) 11, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAF6tIx4030890 11, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 00:55:19 -0600 11, 22 -- Received: from edcexp02.lyondell.com ([161.16.33.40]) 11, 22 -- by edcpap01.lyo.com (8.13.3/8.13.1) with ESMTP id jAF6t9pG021774 11, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 22:55:14 -0800 11, 22 -- Received: from amnhhtv01.millenniumchem.com ([172.19.0.46]) by edcexp02.lyondell.com with Microsoft SMTPSVC(5.0.2195.6713); 11, 22 -- Tue, 15 Nov 2005 00:55:11 -0600 11, 22 -- Subject: INCA Energy software 11, 22 -- To: Microscopy-at-microscopy.com 11, 22 -- X-Mailer: Lotus Notes Release 5.0.11 July 24, 2002 11, 22 -- Message-ID: {OFFCE16D02.CAC8F090-ON482570BA.0023C75C-482570BA.002601C0-at-millenniumche m.com} 11, 22 -- From: alex.titkov-at-millenniumchem.com 11, 22 -- Date: Tue, 15 Nov 2005 14:55:08 +0800 11, 22 -- X-MIMETrack: Serialize by Router on AMNHHTV01/HUB/SRV/MIC(Release 5.0.11 |July 24, 2002) at 11, 22 -- 11/15/2005 01:56:14 AM 11, 22 -- MIME-Version: 1.0 11, 22 -- Content-type: text/plain; charset=us-ascii 11, 22 -- X-OriginalArrivalTime: 15 Nov 2005 06:55:11.0765 (UTC) FILETIME=[8610AC50:01C5E9B1] 11, 22 -- X-Proofpoint-Spam-Details: rule=notspam policy= score=0 mlx=0 adultscore=0 adjust=0 engine=2.5.0-05110701 definitions=3.0.0-05111403 11, 22 -- X-Proofpoint-OriginalSender: alex.titkov-at-millenniumchem.com ==============================End of - Headers==============================
==============================Original Headers============================== 22, 27 -- From eschumacher-at-mccrone.com Tue Nov 15 08:03:20 2005 22, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122] (may be forged)) 22, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAFE3K1H022307 22, 27 -- for {microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 08:03:20 -0600 22, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 22, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 921441A801E 22, 27 -- for {microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 08:03:20 -0600 (CST) 22, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 22, 27 -- by pgp.mccrone.com (PGP Universal service); 22, 27 -- Tue, 15 Nov 2005 08:03:20 -0600 22, 27 -- X-PGP-Universal: processed 22, 27 -- Content-class: urn:content-classes:message 22, 27 -- MIME-Version: 1.0 22, 27 -- Content-Type: text/plain; 22, 27 -- charset="US-ASCII" 22, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 22, 27 -- Subject: RE: [Microscopy] INCA Energy software 22, 27 -- Date: Tue, 15 Nov 2005 08:03:16 -0600 22, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A74C3059-at-MCCRONEMSG.tmg.mccrone.com} 22, 27 -- X-MS-Has-Attach: 22, 27 -- X-MS-TNEF-Correlator: 22, 27 -- Thread-Topic: [Microscopy] INCA Energy software 22, 27 -- Thread-Index: AcXpsbFm/IDZJJgKSQe1omy0B+/TtwAOo97g 22, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 22, 27 -- To: {alex.titkov-at-millenniumchem.com} , {microscopy-at-microscopy.com} 22, 27 -- Content-Transfer-Encoding: 8bit 22, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAFE3K1H022307 ==============================End of - Headers==============================
Thanks to everyone who responded to my question about imaging bacteria. We have a freeze dryer available, and will likely attempt using this first. The best part is that our student has plenty of directions to continue her research.
Karl Hagglund Biological Sciences, SC-102B Northern Kentucky University, Nunn Drive Highland Heights, KY 41099 859-572-5238 http://semlab.nku.edu
==============================Original Headers============================== 3, 23 -- From hagglundk1-at-nku.edu Tue Nov 15 09:02:31 2005 3, 23 -- Received: from mailfe2.nku.edu (mailfe2.nku.edu [192.122.237.68]) 3, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAFF2V4E031813 3, 23 -- for {microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 09:02:31 -0600 3, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211); 3, 23 -- Tue, 15 Nov 2005 10:02:31 -0500 3, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 3, 23 -- Content-class: urn:content-classes:message 3, 23 -- MIME-Version: 1.0 3, 23 -- Content-Type: text/plain; 3, 23 -- charset="us-ascii" 3, 23 -- Subject: Imaging bacteria capsules Thanks 3, 23 -- Date: Tue, 15 Nov 2005 10:02:31 -0500 3, 23 -- Message-ID: {F01E4499C4EC5842A8AB7198141AC72F358584-at-mailfac1.hh.nku.edu} 3, 23 -- X-MS-Has-Attach: 3, 23 -- X-MS-TNEF-Correlator: 3, 23 -- Thread-Topic: Imaging bacteria capsules Thanks 3, 23 -- Thread-Index: AcXp9ZrXrnB5n94QQ0O9YpnkpkyVog== 3, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu} 3, 23 -- To: {microscopy-at-microscopy.com} 3, 23 -- X-OriginalArrivalTime: 15 Nov 2005 15:02:31.0652 (UTC) FILETIME=[9A655A40:01C5E9F5] 3, 23 -- Content-Transfer-Encoding: 8bit 3, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAFF2V4E031813 ==============================End of - Headers==============================
Hi Simon, I have a PIPS which I use for cross sections and the occasional plan view. I find it is not too bad, but I usually have to give the original surface a tickle with the ion beam to clean off contamination. I generally use the standard double-sided holder, not the graphite post (since I have never been very confident about getting the sample off the post again without breaking it!) Milling conditions are double modulation, 3 degrees incidence from below with the top surface up (i.e. facing the viewing port), slowest possible rotation, 6kV. When the sample is thin enough I turn the voltage down to 2.5 kV (the gas flow has to be adjusted to get maximum current) to give a final clean of the milled surface for a minute or two. The top surface clean is also at 2.5 kV but only one burst of the gun, probably about 12 seconds. This has worked quite well, I have identified nm scale contamination on SiO2 films without any real problems with artefacts. It probably helps to stop milling as soon as you get the smallest hole. If I really have to keep the top surface intact I prefer using chemical methods, jet etching with Cl in methanol for III-Vs or HF:HNO3 for Si.
-----Original Message----- X-from: kunli218-at-yahoo.com [mailto:kunli218-at-yahoo.com] Sent: 15 November 2005 13:50 To: Richard Beanland
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kunli218-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kunli218-at-yahoo.com Name: Simon Lee
Organization: CharteredSEMi
Title-Subject: [Filtered] MListserver:Plan view TEM sample prep contamination
Question: Dear Listers,
We met some contamination problem when preparing plan-view TEM sample where the sample can only be milled from one side. We found that the contamination at the unmilled side is quite significant (redeposition). The miller we use is PIPS from Gatan. Will the graphite post help? and Is there any other procedure to minimize the contamination?
==============================Original Headers============================== 11, 12 -- From zaluzec-at-microscopy.com Tue Nov 15 07:48:15 2005 11, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 11, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAFDmE65004244 11, 12 -- for {microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 07:48:15 -0600 11, 12 -- Mime-Version: 1.0 11, 12 -- X-Sender: (Unverified) 11, 12 -- Message-Id: {p06110401bf9f978bf8b8-at-[206.69.208.22]} 11, 12 -- Date: Tue, 15 Nov 2005 07:48:14 -0600 11, 12 -- To: microscopy-at-microscopy.com 11, 12 -- From: kunli218-at-yahoo.com (by way of MicroscopyListserver) 11, 12 -- Subject: viaWWW: Plan view TEM sample prep contamination 11, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
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==============================Original Headers============================== 24, 29 -- From richard.beanland-at-bookham.com Tue Nov 15 09:18:30 2005 24, 29 -- Received: from mail72.messagelabs.com (mail72.messagelabs.com [193.109.255.147]) 24, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jAFFIULN008478 24, 29 -- for {microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 09:18:30 -0600 24, 29 -- X-VirusChecked: Checked 24, 29 -- X-Env-Sender: richard.beanland-at-bookham.com 24, 29 -- X-Msg-Ref: server-10.tower-72.messagelabs.com!1132067908!23632934!1 24, 29 -- X-StarScan-Version: 5.5.9.1; banners=bookham.com,-,- 24, 29 -- X-Originating-IP: [213.249.209.179] 24, 29 -- Received: (qmail 22425 invoked from network); 15 Nov 2005 15:18:28 -0000 24, 29 -- Received: from unknown (HELO cas-smx-brdg-01.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 24, 29 -- by server-10.tower-72.messagelabs.com with SMTP; 15 Nov 2005 15:18:28 -0000 24, 29 -- Content-class: urn:content-classes:message 24, 29 -- MIME-Version: 1.0 24, 29 -- Content-Type: text/plain; 24, 29 -- charset="iso-8859-1" 24, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 24, 29 -- Subject: FW: [Microscopy] viaWWW: Plan view TEM sample prep contamination 24, 29 -- Date: Tue, 15 Nov 2005 15:18:27 -0000 24, 29 -- Message-ID: {FF719652ABB4414D86916ACAAE5BBB24015FBE34-at-cas-smx-01.caswell1.europe.bkhm.net} 24, 29 -- X-MS-Has-Attach: 24, 29 -- X-MS-TNEF-Correlator: 24, 29 -- Thread-Topic: [Microscopy] viaWWW: Plan view TEM sample prep contamination 24, 29 -- Thread-Index: AcXp63vrunVmHvf2T3qTNjyJMEwW/AABSyiwAAHJnPA= 24, 29 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 24, 29 -- To: {microscopy-at-microscopy.com} 24, 29 -- Cc: {kunli218-at-yahoo.com} 24, 29 -- Content-Transfer-Encoding: 8bit 24, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAFFIULN008478 ==============================End of - Headers==============================
For plan view samples I prefer to use the regular post sample holder. I attach the sample with a tiny drop of crystal bond (I mean tiny!), which then gets cleaned off manually with a pointed q-tip and acetone. I do not like to soak off the samples because I feel it contaminates the area you just milled. Also, stop milling when you get the smallest possible hole or it will redep through the hole, and reduce the kV as you get closer to finishing.
If crystal bond residue cannot be tolerated (of course you can't clean it all off), then I use the graphite holder, with a 3mm disc cut from a glass coverslip underneath the sample to protect the surface. The only problem with the graphite holder is the slider bars on either side will block the beam when using rotation only, and the hole will end up somewhat oblong.
If none of that works, you can mill the sample for a few seconds on the 'good' side, but this is hit or miss.
Sincerely, Leslie
Leslie Krupp (Thompson) IBM Almaden Research 650 Harry Road, K19/D2 San Jose, CA 95120-6099
Email: kunli218-at-yahoo.com Name: Simon Lee
Organization: CharteredSEMi
Title-Subject: [Filtered] MListserver:Plan view TEM sample prep contamination
Question: Dear Listers,
We met some contamination problem when preparing plan-view TEM sample where the sample can only be milled from one side. We found that the contamination at the unmilled side is quite significant (redeposition). The miller we use is PIPS from Gatan. Will the graphite post help? and Is there any other procedure to minimize the contamination?
We use the Gatan 655 dry pump station with the TEM specimen holder accessory. It is a bit pricy, but does an outstanding job, and it is very quick and easy to use, with cycle time measured in seconds! http://www.gatan.com/pdf/655%20Dry%20Pumping%20Station.pdf
John Mardinly Intel Corporation
The opinions of this writer do not necessarily represent the opinion of Intel Corporation.
-----Original Message----- X-from: lkrupp-at-us.ibm.com [mailto:lkrupp-at-us.ibm.com] Sent: Monday, November 14, 2005 12:12 PM To: Mardinly, John
Hello-
I was wondering how other listers store their TEM samples between preparation and microscope time. We have oxidation problems, and I am curious whether it is better to store samples under vacuum or an inert gas, as far as preventing/delaying oxidation. Right now we store ours in a cabinet pumped out by a diaphragm pump, and we see contamination even after a couple days on our sensitive samples.
Thank you, Leslie
Leslie Krupp (Thompson) IBM Almaden Research 650 Harry Road, K19/D2 San Jose, CA 95120-6099
==============================Original Headers============================== 4, 27 -- From lkrupp-at-us.ibm.com Mon Nov 14 14:11:36 2005 4, 27 -- Received: from e3.ny.us.ibm.com (e3.ny.us.ibm.com [32.97.182.143]) 4, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEKBaEF010687 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 14:11:36 -0600 4, 27 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236]) 4, 27 -- by e3.ny.us.ibm.com (8.12.11/8.12.11) with ESMTP id jAEKBMEY012768 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 15:11:22 -0500 4, 27 -- Received: from d01av03.pok.ibm.com (d01av03.pok.ibm.com [9.56.224.217]) 4, 27 -- by d01relay04.pok.ibm.com (8.12.10/NCO/VERS6.8) with ESMTP id jAEKBM4S103500 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 15:11:22 -0500 4, 27 -- Received: from d01av03.pok.ibm.com (loopback [127.0.0.1]) 4, 27 -- by d01av03.pok.ibm.com (8.12.11/8.13.3) with ESMTP id jAEKBLst003418 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 15:11:22 -0500 4, 27 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) 4, 27 -- by d01av03.pok.ibm.com (8.12.11/8.12.11) with ESMTP id jAEKBLSV003412 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 15:11:21 -0500 4, 27 -- To: microscopy-at-microscopy.com 4, 27 -- MIME-Version: 1.0 4, 27 -- Subject: TEM sample storage 4, 27 -- X-Mailer: Lotus Notes Release 6.0.2CF1 June 9, 2003 4, 27 -- Message-ID: {OFBDA21EB5.E7D10289-ON852570B9.006DADF5-882570B9.006EE592-at-us.ibm.com} 4, 27 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} 4, 27 -- Date: Mon, 14 Nov 2005 12:11:16 -0800 4, 27 -- X-MIMETrack: Serialize by Router on D01ML604/01/M/IBM(Release 7.0HF60 | October 19, 2005) at 4, 27 -- 11/14/2005 15:11:21, 4, 27 -- Serialize complete at 11/14/2005 15:11:21 4, 27 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
==============================Original Headers============================== 15, 33 -- From john.mardinly-at-intel.com Tue Nov 15 10:40:25 2005 15, 33 -- Received: from scsfmr003.sc.intel.com (fmr23.intel.com [143.183.121.15]) 15, 33 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAFGeOct027462 15, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 15 Nov 2005 10:40:24 -0600 15, 33 -- Received: from scsfmr101.sc.intel.com (scsfmr101.sc.intel.com [10.3.253.10]) 15, 33 -- by scsfmr003.sc.intel.com (8.12.10/8.12.10/d: major-outer.mc,v 1.1 2004/09/17 17:50:56 root Exp $) with ESMTP id jAFGeONV026881; 15, 33 -- Tue, 15 Nov 2005 16:40:24 GMT 15, 33 -- Received: from scsmsxvs040.sc.intel.com (scsmsxvs040.sc.intel.com [10.3.90.8]) 15, 33 -- by scsfmr101.sc.intel.com (8.12.10/8.12.10/d: major-inner.mc,v 1.2 2004/09/17 18:05:01 root Exp $) with SMTP id jAFGbsMm000309; 15, 33 -- Tue, 15 Nov 2005 16:38:13 GMT 15, 33 -- Received: from scsmsx332.amr.corp.intel.com ([10.3.90.6]) 15, 33 -- by scsmsxvs040.sc.intel.com (SAVSMTP 3.1.7.47) with SMTP id M2005111508402319301 15, 33 -- ; Tue, 15 Nov 2005 08:40:23 -0800 15, 33 -- Received: from scsmsx403.amr.corp.intel.com ([10.3.90.18]) by scsmsx332.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.211); 15, 33 -- Tue, 15 Nov 2005 08:40:23 -0800 15, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 15, 33 -- Content-class: urn:content-classes:message 15, 33 -- MIME-Version: 1.0 15, 33 -- Content-Type: text/plain; 15, 33 -- charset="us-ascii" 15, 33 -- Subject: RE: [Microscopy] TEM sample storage 15, 33 -- Date: Tue, 15 Nov 2005 08:40:22 -0800 15, 33 -- Message-ID: {9E1ED6A623D8CB44B4866ACF20ECDB2B08350D90-at-scsmsx403.amr.corp.intel.com} 15, 33 -- X-MS-Has-Attach: 15, 33 -- X-MS-TNEF-Correlator: 15, 33 -- Thread-Topic: [Microscopy] TEM sample storage 15, 33 -- Thread-Index: AcXpV6Q3n3HPpDdYR5qu1e4FEGaHQwAqpUsw 15, 33 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 15, 33 -- To: {lkrupp-at-us.ibm.com} , {Microscopy-at-MSA.Microscopy.Com} 15, 33 -- X-OriginalArrivalTime: 15 Nov 2005 16:40:23.0972 (UTC) FILETIME=[46924640:01C5EA03] 15, 33 -- X-Scanned-By: MIMEDefang 2.52 on 10.3.253.10 15, 33 -- Content-Transfer-Encoding: 8bit 15, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAFGeOct027462 ==============================End of - Headers==============================
I need to verify what do the Net Intensity numbers in the quantification panel represent in the MThin version of the EDAX Genesis 3.6 software.
According to the manuals: "Net Intensity is the Intensity of the peak minus the background after de-convolution".
The problem I have is that using the value of the measured net intensity listed after quantification, I cannot obtain the same number for the Intensity error as listed by the software. I am using the formula: square root of (net intensity multiplied by the live seconds) divided by the (net intensity multiplied by the live seconds).
If I subtract the value of the intensity of the background from the Net Intensity value then I get exactly the number listed as intensity error in the software.
The question is now. Which is correct? The statement that the Net intensity are peak intensity minus the background or that the Net Intensity is the peak intensity + the background. Or may be I am not using the correct calculation procedure for the intensity error?
I have sent this question to EDAX representatives about three weeks ago and I am still waiting for an answer so I decide to "pool the audience" as Regis Philbin likes put it.
_______________________________________ Krassimir N. Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
tel 951 927 2998 fax 951 827 2489 bozhilov-at-ucr.edu _______________________________________
==============================Original Headers============================== 11, 19 -- From bozhilov-at-ucr.edu Tue Nov 15 10:41:02 2005 11, 19 -- Received: from sentry.ucr.edu (sentry.ucr.edu [138.23.226.224]) 11, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAFGf2TE028384 11, 19 -- for {microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 10:41:02 -0600 11, 19 -- Received: from [138.23.185.162] (igppb116g.ucr.edu [138.23.185.162]) 11, 19 -- by sentry.ucr.edu (MOS 3.5.9-GR) 11, 19 -- with ESMTP id DLB93268 (AUTH via LOGINBEFORESMTP) 11, 19 -- for {microscopy-at-microscopy.com} ; 11, 19 -- Tue, 15 Nov 2005 08:41:00 -0800 (PST) 11, 19 -- Mime-Version: 1.0 (Apple Message framework v746.2) 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- Message-Id: {DA3EFD61-FFD6-4814-9AA3-2616944CE8A3-at-ucr.edu} 11, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 11, 19 -- To: microscopy-at-microscopy.com 11, 19 -- From: KN Bozhilov {bozhilov-at-ucr.edu} 11, 19 -- Subject: EDAX Genesis 3.6 Net intensity 11, 19 -- Date: Tue, 15 Nov 2005 08:40:59 -0800 11, 19 -- X-Mailer: Apple Mail (2.746.2) 11, 19 -- X-Junkmail-Status: score=0/65, host=sentry.ucr.edu ==============================End of - Headers==============================
There are a couple of ways to prevent contamination on the back side during ion milling of a plan view sample. The most common way is to use a lacquer that your remove afterwards. The one that I would recommend is MicroShield or Microstop -I can't remember the name for sure and it is available from SPI as well as the remover. Other people have used nail polish. May I recommend Sally Hanson's Hard As Nails?
I can't find the reference for the neatest way that I think is the way to do this. I thought that it was in the first MRS TEM sample Prep book (Vol 115), but I couldn't find it there. What you do is evaporate NaCl in a vacuum evaporator onto the side that you want to protect. Ion mill on the other side. The NaCl layer is the one that is contaminated and after perforation in the ion mill, you simply dip the sample in distilled water to dissolve the NaCl and float off the contamination layer. I wish that I could remember who did that, but I thought that it was a pretty slick idea. If anyone knows the reference or who did it, please let me know because I would like to put that in our application notes section of our web site.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: kunli218-at-yahoo.com [mailto:kunli218-at-yahoo.com] Sent: Tuesday, November 15, 2005 5:53 AM To: Walck-at-SouthBayTech.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both kunli218-at-yahoo.com as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: kunli218-at-yahoo.com Name: Simon Lee
Organization: CharteredSEMi
Title-Subject: [Filtered] MListserver:Plan view TEM sample prep contamination
Question: Dear Listers,
We met some contamination problem when preparing plan-view TEM sample where the sample can only be milled from one side. We found that the contamination at the unmilled side is quite significant (redeposition). The miller we use is PIPS from Gatan. Will the graphite post help? and Is there any other procedure to minimize the contamination?
I just had a EDS system installed on my TEM. As I'm still learning how to use the TEM side of this equation, does anyone have any suggestions on short courses or training I can get so I know what I'm doing?
Thanks in advance!!!
Frank Karl Degussa Corporation Akron Technical Center 3500 Embassy Parkway Suite 100 Akron, Ohio 44333
330-668-2235 Ext. 238
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==============================Original Headers============================== 8, 17 -- From frank.karl-at-degussa.com Tue Nov 15 13:04:52 2005 8, 17 -- Received: from framailout1.rz.itson.com (mailout2.degussa.com [149.216.91.173]) 8, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAFJ4q8g023176 8, 17 -- for {microscopy-at-msa.microscopy.com} ; Tue, 15 Nov 2005 13:04:52 -0600 8, 17 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [172.20.6.74]) 8, 17 -- by framailout1.rz.itson.com (8.13.3/8.13.3/Debian-6) with ESMTP id jAFJ0GD8014598 8, 17 -- for {microscopy-at-msa.microscopy.com} ; Tue, 15 Nov 2005 20:00:16 +0100 8, 17 -- Subject: in search of training.... 8, 17 -- To: microscopy-at-msa.microscopy.com 8, 17 -- X-Mailer: Lotus Notes Release 6.5.2 June 01, 2004 8, 17 -- Message-ID: {OF499F9144.30F0ECE5-ON852570BA.0068532D-852570BA.0068CAB2-at-degussa.com} 8, 17 -- From: frank.karl-at-degussa.com 8, 17 -- Date: Tue, 15 Nov 2005 14:04:36 -0500 8, 17 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 8, 17 -- 11/15/2005 01:04:50 PM 8, 17 -- MIME-Version: 1.0 8, 17 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
A colleague asked me to post the following question. We would appreciate any feedback either directly to me or to the Listserver. Cheers,
"Does any one know the value for GIF collection semiangle for a JEOL 2010F /GIF 2000 (200kV) when operated with an image on the screen, no objective aperture present and a 3mm GIF entrance aperture?
If you have a value, I'd also appreciate the physical dimensions, assumptions and calculations you have made in arriving at it?
Many thanks in advance."
Mark Blackford Institute of Materials and Engineering Science, ANSTO PMB 1, Menai, N.S.W., 2234 Australia
Phone 61 2 9717 3027 Fax 61 2 9543 7179
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==============================Original Headers============================== 12, 24 -- From mgb-at-ansto.gov.au Tue Nov 15 16:36:31 2005 12, 24 -- Received: from tachyon.gw.ansto.gov.au (tachyon.gw.ansto.gov.au [137.157.8.253]) 12, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAFMaTM4002454 12, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 16:36:30 -0600 12, 24 -- Received: (from uucp-at-localhost) 12, 24 -- by tachyon.gw.ansto.gov.au (8.11.7p1+Sun/8.11.7) id jAFMaNY07227 12, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Nov 2005 09:36:23 +1100 (EST) 12, 24 -- Received: from jenner.ansto.gov.au(137.157.59.25) by tachyon.gw.ansto.gov.au via csmap (V6.0) 12, 24 -- id srcAAAmLaiho; Wed, 16 Nov 05 09:36:23 +1100 12, 24 -- Received: from hadron.ansto.gov.au (hadron.ansto.gov.au [137.157.13.219]) 12, 24 -- by jenner.ansto.gov.au (8.12.10/8.12.10) with ESMTP id jAFMaJtT009634 12, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Nov 2005 09:36:19 +1100 12, 24 -- Received: from [137.157.94.43] (m0365m.ansto.gov.au [137.157.94.43]) 12, 24 -- by hadron.ansto.gov.au (8.9.3/8.9.3) with ESMTP id JAA17380 12, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Nov 2005 09:36:18 +1100 (EST) 12, 24 -- Mime-Version: 1.0 12, 24 -- X-Sender: mgb-at-pop.ansto.gov.au (Unverified) 12, 24 -- Message-Id: {a06110400bfa01133d1c3-at-[137.157.94.43]} 12, 24 -- Date: Wed, 16 Nov 2005 09:33:22 +1100 12, 24 -- To: Microscopy-at-microscopy.com 12, 24 -- From: Mark Blackford {mgb-at-ansto.gov.au} 12, 24 -- Subject: GIF collection semi-angle 12, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 12, 24 -- X-ANSTO-MailScanner: Found to be clean ==============================End of - Headers==============================
I am not sure how to write this so that it does not sound like too much of a commercial. I will put the disclaimer up front so that you can choose to read on and I will try to be as factual about the product as I can without too much commercialism and hope that I don't get spanked by Nestor. I think that I am within the guidelines because what I am relating below is the design considerations and features that evolved out of solving the solution to my microscopy studies that I have not reported on before.
Disclaimer: South Bay Technology manufactures and sells the SampleSaver(TM) Portable Storage Container that addresses the problem of transportation and storage of samples or samples with coatings that react when exposed to the atmosphere. This product was just introduced at the M&M 2005 meeting and other than that and the recent ISFTA 2005 meeting, we have not advertised it. In response to this question, we just put the brochure for the unit on our web site in order so that anyone interested can view what the unit looks like. This unit was specifically made to address the general topic of this question.
The SampleSaver(TM) portable storage container (SS) is my first contribution to the SBT product line and came about because of problems that I had with reactive samples when I worked at Wright Patterson Air Force Base and at PPG Industries, Inc. It is a plastic container in which there are two valves, one of which is a conventional valve and one which is integral into the body of the unit, itself. Metallurgical, SEM, and TEM samples can be held inside with different "sample trees". There is also a special unit that we have that is made for FIB lift-out samples. The SS is designed to be purged without pumping it down with an inert gas that could be N2, Ar, or CO2 from a cylinder or from a special unit, which we also sell, will use the boil-off from liquid nitrogen. The idea of using the boil-off from liquid nitrogen (or the CO2 gas from Dry Ice) is that if you prepare your samples elsewhere and travel to another lab to do the analysis or vice versa (e.g. when you FIB your samples at another lab and bring them home) the other lab will always have liquid nitrogen available to use as the source for the inert gas. They may or may not have a vacuum pump, Ar line, or a N2 line available for use. One major benefit of using LN2, is that the boil-off from LN2 is extremely pure. When the SS unit is sufficiently purged, the two valves are closed, body valve first then the gas supply valve. The valve body can then be compressed. This then pressurizes the container to help prevent ingress of any gas species by diffusion through the plastic unit. The unit can be tested for integrity just before opening by opening the valve while it is compressed. A "psst" sound is reassuring. A tube from the valve can be put under LN2 so that the container is not exposed to air.
I have images of XTEM samples of Low-E coatings on glass that have been preserved using this system that I could share with anyone that is interested. These Low-E coatings contain two layers of silver that when the two surfaces of the cross section are exposed degrade in air over a short period of time. Samples that have been stored for many weeks have not undergone any degradation.
The unit was also developed for SEM samples that would be coated with chromium where the samples would otherwise oxidize in a relative short period. It was also intended for EBSD applications of metallurgical materials where surface oxidation after preparation would degrade the EBSD results.
Although I designed this only to be used in a purging mode because a vacuum pump would be too bulky for portability and you can't guarantee that one is available at the other lab, it can also be used with a vacuum pump, if desired, and a pump-backfill sequence is possible with it. I don't recommend leaving it in a vacuum condition, because of the possible diffusion of species such as O2 and H2 through the plastic. The positive pressure within the SS with an inert gas inhibits this diffusion.
The SS can also be used with glove boxes such as the type that was introduced by Omniprobe at M&M 2005. Here, the unit is simply put inside the glove box and when the glove box is sufficiently purged, samples can be put into it or removed from it and then reclosed. By compressing the body, the pressure inside the SS will be higher than the pressure in the glove box.
What we don't know at this time is whether samples stored in it and used with it will require plasma cleaning for high resolution work. My results have been good and I have not seen any contamination when used with a LaB6 microscope. I have transported samples to field emission microscopes and have not seen contamination issues. I suspect that the higher pressure of the inert gas inside the SS may prevent or inhibit any out gassing from the plastic body, but I am only guessing here.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: lkrupp-at-us.ibm.com [mailto:lkrupp-at-us.ibm.com] Sent: Monday, November 14, 2005 12:15 PM To: Walck-at-SouthBayTech.com
Hello-
I was wondering how other listers store their TEM samples between preparation and microscope time. We have oxidation problems, and I am curious whether it is better to store samples under vacuum or an inert gas, as far as preventing/delaying oxidation. Right now we store ours in a cabinet pumped out by a diaphragm pump, and we see contamination even after a couple days on our sensitive samples.
Thank you, Leslie
Leslie Krupp (Thompson) IBM Almaden Research 650 Harry Road, K19/D2 San Jose, CA 95120-6099
==============================Original Headers============================== 4, 27 -- From lkrupp-at-us.ibm.com Mon Nov 14 14:11:36 2005 4, 27 -- Received: from e3.ny.us.ibm.com (e3.ny.us.ibm.com [32.97.182.143]) 4, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAEKBaEF010687 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 14:11:36 -0600 4, 27 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236]) 4, 27 -- by e3.ny.us.ibm.com (8.12.11/8.12.11) with ESMTP id jAEKBMEY012768 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 15:11:22 -0500 4, 27 -- Received: from d01av03.pok.ibm.com (d01av03.pok.ibm.com [9.56.224.217]) 4, 27 -- by d01relay04.pok.ibm.com (8.12.10/NCO/VERS6.8) with ESMTP id jAEKBM4S103500 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 15:11:22 -0500 4, 27 -- Received: from d01av03.pok.ibm.com (loopback [127.0.0.1]) 4, 27 -- by d01av03.pok.ibm.com (8.12.11/8.13.3) with ESMTP id jAEKBLst003418 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 15:11:22 -0500 4, 27 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) 4, 27 -- by d01av03.pok.ibm.com (8.12.11/8.12.11) with ESMTP id jAEKBLSV003412 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 15:11:21 -0500 4, 27 -- To: microscopy-at-microscopy.com 4, 27 -- MIME-Version: 1.0 4, 27 -- Subject: TEM sample storage 4, 27 -- X-Mailer: Lotus Notes Release 6.0.2CF1 June 9, 2003 4, 27 -- Message-ID: {OFBDA21EB5.E7D10289-ON852570B9.006DADF5-882570B9.006EE592-at-us.ibm.com} 4, 27 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} 4, 27 -- Date: Mon, 14 Nov 2005 12:11:16 -0800 4, 27 -- X-MIMETrack: Serialize by Router on D01ML604/01/M/IBM(Release 7.0HF60 | October 19, 2005) at 4, 27 -- 11/14/2005 15:11:21, 4, 27 -- Serialize complete at 11/14/2005 15:11:21 4, 27 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
==============================Original Headers============================== 21, 23 -- From walck-at-southbaytech.com Tue Nov 15 17:05:47 2005 21, 23 -- Received: from pimout7-ext.prodigy.net (pimout7-ext.prodigy.net [207.115.63.58]) 21, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAFN5k8Z011471 21, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 17:05:47 -0600 21, 23 -- X-ORBL: [64.169.193.90] 21, 23 -- Received: from dynamicbl8uno3 (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90]) 21, 23 -- by pimout7-ext.prodigy.net (8.13.4 outbound domainkey aix/8.13.4) with ESMTP id jAFN0pTq114584 21, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 18:01:00 -0500 21, 23 -- From: "Scott Walck" {walck-at-southbaytech.com} 21, 23 -- To: {Microscopy-at-microscopy.com} 21, 23 -- Subject: RE: [Microscopy] TEM sample storage 21, 23 -- Date: Tue, 15 Nov 2005 15:00:57 -0800 21, 23 -- Message-ID: {001901c5ea38$760563a0$7801a8c0-at-dynamicbl8uno3} 21, 23 -- MIME-Version: 1.0 21, 23 -- Content-Type: text/plain; 21, 23 -- charset="us-ascii" 21, 23 -- Content-Transfer-Encoding: 7bit 21, 23 -- X-Priority: 3 (Normal) 21, 23 -- X-MSMail-Priority: Normal 21, 23 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 21, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 21, 23 -- In-Reply-To: {200511142014.jAEKEx6v017318-at-ns.microscopy.com} 21, 23 -- Importance: Normal ==============================End of - Headers==============================
After discovering that many of our older toluidine blue stained semi-thin sections that had been mounted with a commercial mounting medium had faded, I decided to try using our standard Epon-Araldite-DDSA embedding resin as a mounting medium. Simply put a drop or two of resin (we use left over resin from embeddings, stored in vials in a freezer) on the dry, stained slides, and coverslip slowly to avoid bubbles. The slides are viewable immediately, and will harden in a few days on their own, or overnight at 60 degrees. They should not be overheated or some destaining or wrinkling may occur. Since switching to Epon as a mounting medium, I have not seen any problem with fading.
As for viewing slides before permanent mounting, the image can be greatly improved just by placing a coverslip over the dry section while viewing. Most objectives are corrected for the presence of a coverslip, so even with nothing but air between the coverslip and the section, the image is very good.
Ralph Common Division of Human Pathology Michigan State University
==============================Original Headers============================== 4, 24 -- From rcommon-at-msu.edu Tue Nov 15 18:01:36 2005 4, 24 -- Received: from sys16.mail.msu.edu (sys16.mail.msu.edu [35.9.75.116]) 4, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAG01aqZ021005 4, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 18:01:36 -0600 4, 24 -- Received: from [35.9.122.125] (helo=emlab) 4, 24 -- by sys16.mail.msu.edu with esmtpsa (Exim 4.52 #1) 4, 24 -- (TLSv1:RC4-MD5:128) 4, 24 -- id 1EcAk4-0007Nj-5i 4, 24 -- for Microscopy-at-microscopy.com; Tue, 15 Nov 2005 19:01:36 -0500 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: Fading Sections 4, 24 -- Date: Tue, 15 Nov 2005 19:01:57 -0500 4, 24 -- Message-ID: {001001c5ea40$f6ae1ee0$7d7a0923-at-msu.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="iso-8859-1" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 (Normal) 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 4, 24 -- Importance: Normal 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1441 4, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
Thanks everyone. I shall chase issue 16 then. Forgot to mention that we are in Australia, not in the States as some people thought.
Cheers, Alex
================== Alexander Titkov Millennium Inorganic Chemicals Ltd A Lyondell Company Locked Bag 245 Bunbury WA 6230 AUSTRALIA Ph 08 9780 8505 FAX 08 9780 8500 E-mail: alex.titkov-at-millenniumchem.com
eschumacher-at-mccro ne.com To: alex.titkov-at-millenniumchem.com cc: 15/11/2005 10:06 Subject: [Microscopy] RE: INCA Energy software PM Please respond to eschumacher
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi Alexander,
I just received Issue 16 last week as a service contract upgrade. We've always gotten prompt responses on software and other issues from our local service and sales reps and from the MA office, with additional support from the UK if necessary. Getting the upgrade with your service contract renewal should be straightforward.
Regards,
Elaine
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
-----Original Message----- X-from: alex.titkov-at-millenniumchem.com [mailto:alex.titkov-at-millenniumchem.com] Sent: Tuesday, November 15, 2005 12:56 AM To: Elaine F. Schumacher
Hi,
Can anyone please tell me what the latest issue of The Microanalysis Suite from Oxford Instruments is currently available? What issue are you using? We are on issue 13. Our service contract entitles us for free software upgrades, but we just can not get the information from the local Oxford representatives.
Many thanks, Alexander Titkov
Millennium Inorganic Chemicals Ltd A Lyondell Company
Lyondell Chemical Company
The information contained in this e-mail message and any attachments may be confidential. It is intended only for the use of the individual or entities named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail at the originating address.
==============================Original Headers============================== 11, 22 -- From alex.titkov-at-millenniumchem.com Tue Nov 15 00:55:20 2005 11, 22 -- Received: from edcpap01.lyo.com (mail3.lyondell.com [161.16.0.77]) 11, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAF6tIx4030890 11, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 00:55:19 -0600 11, 22 -- Received: from edcexp02.lyondell.com ([161.16.33.40]) 11, 22 -- by edcpap01.lyo.com (8.13.3/8.13.1) with ESMTP id jAF6t9pG021774 11, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Nov 2005 22:55:14 -0800 11, 22 -- Received: from amnhhtv01.millenniumchem.com ([172.19.0.46]) by edcexp02.lyondell.com with Microsoft SMTPSVC(5.0.2195.6713); 11, 22 -- Tue, 15 Nov 2005 00:55:11 -0600 11, 22 -- Subject: INCA Energy software 11, 22 -- To: Microscopy-at-microscopy.com 11, 22 -- X-Mailer: Lotus Notes Release 5.0.11 July 24, 2002 11, 22 -- Message-ID: {OFFCE16D02.CAC8F090-ON482570BA.0023C75C-482570BA.002601C0-at-millenniumche m.com} 11, 22 -- From: alex.titkov-at-millenniumchem.com 11, 22 -- Date: Tue, 15 Nov 2005 14:55:08 +0800 11, 22 -- X-MIMETrack: Serialize by Router on AMNHHTV01/HUB/SRV/MIC(Release 5.0.11 |July 24, 2002) at 11, 22 -- 11/15/2005 01:56:14 AM 11, 22 -- MIME-Version: 1.0 11, 22 -- Content-type: text/plain; charset=us-ascii 11, 22 -- X-OriginalArrivalTime: 15 Nov 2005 06:55:11.0765 (UTC) FILETIME=[8610AC50:01C5E9B1] 11, 22 -- X-Proofpoint-Spam-Details: rule=notspam policy= score=0 mlx=0 adultscore=0 adjust=0 engine=2.5.0-05110701 definitions=3.0.0-05111403 11, 22 -- X-Proofpoint-OriginalSender: alex.titkov-at-millenniumchem.com ==============================End of - Headers==============================
==============================Original Headers ============================== 22, 27 -- From eschumacher-at-mccrone.com Tue Nov 15 08:03:20 2005 22, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122] (may be forged)) 22, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAFE3K1H022307 22, 27 -- for {microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 08:03:20 -0600 22, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 22, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 921441A801E 22, 27 -- for {microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 08:03:20 -0600 (CST) 22, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 22, 27 -- by pgp.mccrone.com (PGP Universal service); 22, 27 -- Tue, 15 Nov 2005 08:03:20 -0600 22, 27 -- X-PGP-Universal: processed 22, 27 -- Content-class: urn:content-classes:message 22, 27 -- MIME-Version: 1.0 22, 27 -- Content-Type: text/plain; 22, 27 -- charset="US-ASCII" 22, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 22, 27 -- Subject: RE: [Microscopy] INCA Energy software 22, 27 -- Date: Tue, 15 Nov 2005 08:03:16 -0600 22, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A74C3059-at-MCCRONEMSG.tmg.mccrone.com} 22, 27 -- X-MS-Has-Attach: 22, 27 -- X-MS-TNEF-Correlator: 22, 27 -- Thread-Topic: [Microscopy] INCA Energy software 22, 27 -- Thread-Index: AcXpsbFm/IDZJJgKSQe1omy0B+/TtwAOo97g 22, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 22, 27 -- To: {alex.titkov-at-millenniumchem.com} , {microscopy-at-microscopy.com} 22, 27 -- Content-Transfer-Encoding: 8bit 22, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAFE3K1H022307 ==============================End of - Headers ==============================
Lyondell Chemical Company
The information contained in this e-mail message and any attachments may be confidential. It is intended only for the use of the individual or entities named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail at the originating address.
==============================Original Headers============================== 48, 22 -- From alex.titkov-at-millenniumchem.com Tue Nov 15 21:34:09 2005 48, 22 -- Received: from edcpap01.lyo.com (mail3.lyondell.com [161.16.0.77]) 48, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAG3Y6dY002184 48, 22 -- for {microscopy-at-microscopy.com} ; Tue, 15 Nov 2005 21:34:08 -0600 48, 22 -- Received: from edcexp02.lyondell.com ([161.16.33.40]) 48, 22 -- by edcpap01.lyo.com (8.13.3/8.13.1) with ESMTP id jAG3Xrd3013184; 48, 22 -- Tue, 15 Nov 2005 19:33:57 -0800 48, 22 -- Received: from amnhhtv01.millenniumchem.com ([172.19.0.46]) by edcexp02.lyondell.com with Microsoft SMTPSVC(5.0.2195.6713); 48, 22 -- Tue, 15 Nov 2005 21:33:55 -0600 48, 22 -- Subject: Re: [Microscopy] RE: INCA Energy software 48, 22 -- To: eschumacher-at-mccrone.com, {microscopy-at-microscopy.com} 48, 22 -- X-Mailer: Lotus Notes Release 5.0.11 July 24, 2002 48, 22 -- Message-ID: {OF1E4165C8.FAE282BE-ON482570BB.00131804-482570BB.001393FE-at-millenniumchem.com} 48, 22 -- From: alex.titkov-at-millenniumchem.com 48, 22 -- Date: Wed, 16 Nov 2005 11:33:50 +0800 48, 22 -- X-MIMETrack: Serialize by Router on AMNHHTV01/HUB/SRV/MIC(Release 5.0.11 |July 24, 2002) at 48, 22 -- 11/15/2005 10:34:58 PM 48, 22 -- MIME-Version: 1.0 48, 22 -- Content-type: text/plain; charset=us-ascii 48, 22 -- X-OriginalArrivalTime: 16 Nov 2005 03:33:55.0288 (UTC) FILETIME=[92564580:01C5EA5E] 48, 22 -- X-Proofpoint-Spam-Details: rule=notspam policy= score=0 mlx=0 adultscore=0 adjust=0 engine=2.5.0-05110701 definitions=3.0.0-05111505 48, 22 -- X-Proofpoint-OriginalSender: alex.titkov-at-millenniumchem.com ==============================End of - Headers==============================
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Question: Dear colleagues, I would like to ask if someone of you know conferences involving (environmental) electron microscopy and biomaterials or related biological materials taking place in 2006. Thank you very much.
The Fall Symposium of the New England Society for Microscopy will once again be held at Gordon College in Wenham, MA. It will be held on Thursday, December 1st from Noon - 8:30pm. The program highlights talks on various microscopy techniques. For more detailed information, including registration, the speakers and talks, please go to NESM's website: http://nesm.cims.harvard.edu and click on "current newsletter".
The deadline for registration (including dinner) is Monday, November 28th. Please contact Paul Bain, Treasurer at Paul_Bain-at-hms.harvard.edu.
We look forward to seeing you at the meeting!
Peggy Sherwood Corresponding Secretary, NESM
Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood-at-partners.org
==============================Original Headers============================== 12, 23 -- From MSHERWOOD-at-PARTNERS.ORG Wed Nov 16 08:23:45 2005 12, 23 -- Received: from PHSXCON5.partners.org (phsxcon5.mgh.harvard.edu [132.183.130.38]) 12, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAGENio2028960 12, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Nov 2005 08:23:44 -0600 12, 23 -- Received: from PHSXMB1.partners.org ([132.183.118.117]) by PHSXCON5.partners.org with Microsoft SMTPSVC(6.0.3790.211); 12, 23 -- Wed, 16 Nov 2005 09:23:44 -0500 12, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 12, 23 -- Content-class: urn:content-classes:message 12, 23 -- MIME-Version: 1.0 12, 23 -- Content-Type: text/plain; 12, 23 -- charset="iso-8859-1" 12, 23 -- Subject: [Microscopy] re: New England Society for Microscopy (NESM) Fall Symposium 12, 23 -- Date: Wed, 16 Nov 2005 09:23:44 -0500 12, 23 -- Message-ID: {1AF23D0AD12E7444A5DB083CA978B73403F927E3-at-PHSXMB1.partners.org} 12, 23 -- X-MS-Has-Attach: 12, 23 -- X-MS-TNEF-Correlator: 12, 23 -- Thread-Topic: [Microscopy] re: New England Society for Microscopy (NESM) Fall Symposium 12, 23 -- Thread-Index: AcXquVmODn/NEs7iS2uFv0Gs6PZNrA== 12, 23 -- From: "Sherwood, Margaret " {MSHERWOOD-at-PARTNERS.ORG} 12, 23 -- To: {Microscopy-at-microscopy.com} 12, 23 -- X-OriginalArrivalTime: 16 Nov 2005 14:23:44.0558 (UTC) FILETIME=[59C0BCE0:01C5EAB9] 12, 23 -- Content-Transfer-Encoding: 8bit 12, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAGENio2028960 ==============================End of - Headers==============================
I am looking for a technique that will help identify starch in osmicated/Embed-Araldite embedded sections. One suggestion has been to use amylase digestion or amylase/gold. Any advice or other suggestions would be greatly appreciated.
-- Karen L. Kelley ICBR Electron Microscopy Manager University of Florida ICBR Electron Microscopy Core Lab Bartram Hall Room 214 Box 118525 Gainesville Florida Lab: 352-392-1184 fax: 352-846-0251 Email: klk-at-biotech.ufl.edu Southeastern Microscopy Society Treasurer http://www.biotech.ufl.edu/EM/
==============================Original Headers============================== 4, 21 -- From klk-at-biotech.ufl.edu Wed Nov 16 14:23:28 2005 4, 21 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 4, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAGKNQbT010186 4, 21 -- for {microscopy-at-microscopy.com} ; Wed, 16 Nov 2005 14:23:27 -0600 4, 21 -- Received: from [128.227.60.41] (mulkey-prn.botany.ufl.edu [128.227.60.41]) 4, 21 -- by smtp.ufl.edu (8.13.4/8.13.4/2.5.1) with ESMTP id jAGKNNEG1077416 4, 21 -- for {microscopy-at-microscopy.com} ; Wed, 16 Nov 2005 15:23:24 -0500 4, 21 -- Message-ID: {437B953B.4040301-at-biotech.ufl.edu} 4, 21 -- Date: Wed, 16 Nov 2005 15:23:23 -0500 4, 21 -- From: Karen Kelley {klk-at-biotech.ufl.edu} 4, 21 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 4, 21 -- X-Accept-Language: en-us, en 4, 21 -- MIME-Version: 1.0 4, 21 -- To: microscopy-at-microscopy.com 4, 21 -- Subject: starch in sections 4, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 21 -- Content-Transfer-Encoding: 7bit 4, 21 -- X-Spam-Status: hits=-1.428, required=5, tests=BAYES_20 4, 21 -- X-UFL-Spam-Status: hits=-1.428, required=5, tests=BAYES_20 4, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 4, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
Hi Karen, Starch grains are beautifully birefringent so they show up a treat in polarized light microscopy. If you can use semi thin sections to find/demonstrate them, you would be home free. In an ultra-thin section, the polarized light signal would be low, so you might need a sensitive instrument to detect them but I think still quite possible. Of course this is at the light level, not TEM. Hope it helps.
Tobias Baskin
} } } I am looking for a technique that will help identify starch in } osmicated/Embed-Araldite embedded sections. One suggestion has been to } use amylase digestion or amylase/gold. Any advice or other suggestions } would be greatly appreciated. } } -- } Karen L. Kelley } ICBR Electron Microscopy Manager } University of Florida } ICBR Electron Microscopy Core Lab } Bartram Hall Room 214 } Box 118525 Gainesville Florida } Lab: 352-392-1184 fax: 352-846-0251 } Email: klk-at-biotech.ufl.edu } Southeastern Microscopy Society Treasurer } http://www.biotech.ufl.edu/EM/ } } } } =======
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Email: barbara.miner-at-intel.com Name: Miner, Barbara
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Title-Subject: [Filtered] Experienced TEM sample prep technician
Question: Job Title: experienced TEM sample prep technician Location: Hillsboro, OR Start date: December 2005 or January 2006 Requisition will close at the end of November
Intel Corporationís Materials Analysis Lab, supporting development of next generation micro-processors, has immediate openings for experienced Transmission Electron Microscopy sample prep technicians in Hillsboro, Oregon. ÝÝSuccessful applicants must have experience working with patterned Si wafers, operating dual-beam FIBs, and applying precision mechanical polish. Candidate must have excellent attention to detail and the ability to multi-task. Good communication, problem-solving skills, and cooperative team work are mandatory. The work shift includes one weekend day. Relocation assistance is provided.
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Karl, Just to offer a heretically simplifed option for quick-and-dirty (but often unexpectedly clean) speedy results: I agree with Muss that tannic acid is great, the surest simple fixative, and with Webster that freezing is a promising approach.
But I suggest that for fixing extracellular matrix, and intracellular structures of detergent (TX-100) pre-permeabilized cells, you can do quite well without OsO4; just use freshly dissolved 0.2% TA in your physiol extracellular or intracellular buffer at pH 6.8-7.0 (absent any components that precipitate in TA) for 30 min, then wash out all unbound TA in 5 buffer changes, and follow with 1% UrAc in DI water, no pH adjustment needed, then do acetone or EtOH dehydration as fast as you like. If you wish, you can interpose 0.5-2.0 hr in 1% glutaraldehyde after the TA and before the UrAc, but i usually use straight TAURAC as a preferred binary fix; is certainly less toxic and just as fixing as a similar TAOS procedure. (M. K. Reedy, C. Lucaveche, D. Popp, Biophysical Journal 59, 579a (1991)). Worth one shot for comparison is using a TA-glut mixture as a trial primary fix before UrAc, but I find glut sometimes tends to rush in and perturb orderly lattice arrays that TA alone fixes more slowly and beautifully. TA primary fix MUST be stabilized by UrAc or OsO4 secondary fix before dehydration.
I've used the same TAURAC sequence in cryo-acetone for freeze-substitution almost exclusively for 15 years. that's what suggests to me its possible value for polysaccharides. It is able to fix and stabilize (or perhaps it just permits PHYSICAL in-situ stabilizing of) the threadlike molecules of 500,000 MW dextran we sometimes use at 3-5% as an osmotic squeezer for the myofilament lattice of glycerinated muscle fibers. We can see them in thin sections, excluded to the space between myofibrils. However, the aqueous TAURAC fix does NOT preserve /retain the free dextran molecules, so I guess the quick-freezing and/or acetone is needed for that.
So I guess maybe using an initial non-cryo "fix" of acetone alone, followed by TAURAC in acetone and comparing with acetone-TAURAC sequence and with an acetone-URAC-TA sequence might be worth trying. Note Craig lab's findings that UrAc alone can be a fabulous fixative. (F. Q. Zhao, R. Craig, Journal of Structural Biology 141, 43 (Jan, 2003).
And if you want to try a totally safer alternative than propane for the plunge-freezing that Paul Webster suggests, LN2 plunging has a bad rap that is undeserved. If you plunge rapidly through 10-12 inches of LN2, you leave behind all the insulating bubbles responsible for the Leidenfrost effect and get convective cooling at least as rapid or more-so than propane provides. See what protein crystallographers from Hope's lab have to say about how reliable this can be! (L. J. Walker, P. O. Moreno, H. Hope, Journal of Applied Crystallography 31, 954 (1998); S. Parkin, H. Hope, Journal of Applied Crystallography 31, 945 (1998)).
Best of luck! -- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
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Email: justgetmeonhere-at-netscape.net Name: Matthew McCaskill
Organization: none
Title-Subject: [Filtered] question about BSE detectors
Question: Hello all,
I am enrolled in an SEM class, and for this class I have been asked to do a paper on backscattered electron (BSE) detectors. Specifically, I have to briefly state what is state-of-the-art now, and then discuss research or improvements that are occurring right now. Then I am to predict and forecast the future of the technology; will there be more developments, or have BSE detectors fully matured technologically?
The point of all this post, however, is that I'm having trouble finding literature on SEM in general, let alone BSE detectors. Utilizing my school's library and the internet, I have found at least four articles already, but I need at least four more. Can anybody recommend me some journal articles or papers that deal with my topic? Preferably, I'd like for them to be available as FREE electronic copies (I found a few promising articles online, but unfortunately I have to pay to view, which is disadvantageous to poor college students such as myself), but if I can get ahold of the actual hard copies at my school's library then that should also be enough.
Thanks to all who can help me in this regard, and thanks to all of you for your time.
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Title-Subject: [Filtered] Post Doc Position Open - ultrastructural biology
Question: A postdoctoral position (Level A/B) is available to work on ultrastructural biology in a new ARC Centre of Excellence for Coherent X-ray Science. ( {http://www.coecxs.org/http://www.coecxs.org/). This position is funded by the Australian Research Council as part of the Centre of Excellence for Coherent X-ray Science for research into the use of novel X-ray diffraction techniques to study the cellular architecture of malaria parasite-infected erythrocytes and other biological samples. The project involves adapting sample preparation methods developed for electron microscopy to generate samples suitable for X-ray microscopy and other X-ray imaging techniques. The successful applicant will work with colleagues from the Departments of Biochemistry and Physics, at La Trobe University, and other Centre members to image the ultrastructure of malaria parasites and other samples. The applicant will also prepare samples for cyro-electron microscopy and single particle image reconstruction techniques. Molecular biological manipulation of cells and proteins will also be used in enhancing sample preparation.
Further information, contact: Prof. Leann Tilley. Department of Biochemistry, La Trobe University. Tel: 9479 1375. Email: {mailto:L.Tilley-at-latrobe.edu.auL.Tilley-at-latrobe.edu.au
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To be held in conjunction with the March 24, 2006 meeting of the Midwest
Microscopy and Microanalysis Society
Affiliate of the Microscopy Society of America and the Microbeam Analysis Society of America
ABSTRACT DEADLINE: Abstracts must be received in electronic format by 5PM, Friday, December 16, 2005.
The first quarter meeting of the Midwest Microscopy and Microanalysis Society will be held on Friday, March 24, 2006, in the Pancoe Life Sciences Building, Northwestern University, Evanston, Illinois. A student poster competition open to undergraduate and graduate students will be held in conjunction with the meeting. Posters should illustrate utilization of microscopy for either biological or materials science study. Prizes will be awarded as follows:
$300 for 1st place $200 for 2nd place $100 for 3rd place
Abstracts must be received in electronic format by 5PM on Friday, December 16, 2005. To be eligible for a prize, you must be first author on the poster, and you must be present at the meeting. You are encouraged to submit your entry as early as possible, as space may be limited. Abstracts from last year's competition, and an example of the judging worksheet can be found on the MMMS website:
Matthew - I would contact the SEM manufacturer, JEOL (978-535-5900), and the EDS manufacturers EDAX (201-529-4880) and PGT (609-924-7310), or check their websites; I have seen technical articles regarding this authored by their employees, and there are probably other SEM/EDS companies with helpful literature as well.
Jane L. LaGoy Lab Manager/R&D Engineer 978-470-1620 x450 jane.lagoy-at-bodycote.com
BODYCOTE NORTH AMERICA · 155 RIVER STREET · ANDOVER · MASSACHUSETTS · 01810 TEL: (978) 470-1620 · FAX: (978) 475-2951 · ONLINE: www.bodycote.com
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Email: justgetmeonhere-at-netscape.net Name: Matthew McCaskill
Organization: none
Title-Subject: [Filtered] question about BSE detectors
Question: Hello all,
I am enrolled in an SEM class, and for this class I have been asked to do a paper on backscattered electron (BSE) detectors. Specifically, I have to briefly state what is state-of-the-art now, and then discuss research or improvements that are occurring right now. Then I am to predict and forecast the future of the technology; will there be more developments, or have BSE detectors fully matured technologically?
The point of all this post, however, is that I'm having trouble finding literature on SEM in general, let alone BSE detectors. Utilizing my school's library and the internet, I have found at least four articles already, but I need at least four more. Can anybody recommend me some journal articles or papers that deal with my topic? Preferably, I'd like for them to be available as FREE electronic copies (I found a few promising articles online, but unfortunately I have to pay to view, which is disadvantageous to poor college students such as myself), but if I can get ahold of the actual hard copies at my school's library then that should also be enough.
Thanks to all who can help me in this regard, and thanks to all of you for your time.
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Email: apnewell-at-ncsu.edu Name: AndyNewell
Organization: AREMC / NC State
Title-Subject: [Filtered] low voltage STEM
Question: I am looking for literature on current developments of SEM-based STEM (low kV) and/or names of predominant researchers that have benefitted recently by using STEM in an SEM for thin sample characterization and microanalysis. Information on other improvements such as aberration corrections that have helped low-voltage STEM or speculation on the next step in low energy STEM development is also welcome.
Many of us graduate students here at NCSU are having difficulty finding publications on development of the technology due to the proprietary nature of such developments, but I am sure there is some fine work going on in the scientififc community. Can anyone help me locate the works of some SEM-based STEM heavy-hitters?
Any assistance available is greatly appreciated.
Many thanks, Andy Newell Materials Science & Engineering NC State University
l have been trying to look at some bug fossils in rock samples with SEM. In hand specimen these bugs stand out as dark areas against light tan rock material. In the SEM using backscatter they tend to fade into the background which seems to say there is not much organic material left. SEM secondary electron images do not yeild much information either.
Would like to hear any ideas on other techniques people have use to image such samples.
Thanks
Roy Beavers
Southern Methodist University Department of Geological Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 8, 20 -- From rbeavers-at-mail.smu.edu Fri Nov 18 11:51:30 2005 8, 20 -- Received: from s31xe5.systems.smu.edu (s31xe5.systems.smu.edu [129.119.70.74]) 8, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAIHpU86001271 8, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Nov 2005 11:51:30 -0600 8, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 8, 20 -- Content-class: urn:content-classes:message 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Type: text/plain; 8, 20 -- charset="iso-8859-1" 8, 20 -- Subject: Imaging bug fossils 8, 20 -- Date: Fri, 18 Nov 2005 11:51:28 -0600 8, 20 -- Message-ID: {3A9F6E299461A44483961A57175598A703402CAA-at-s31xe5.systems.smu.edu} 8, 20 -- X-MS-Has-Attach: 8, 20 -- X-MS-TNEF-Correlator: 8, 20 -- Thread-Topic: Imaging bug fossils 8, 20 -- thread-index: AcXsaLN/mflZ3DQtQ66cS0a1xut+XQ== 8, 20 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 8, 20 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAIHpU86001271 ==============================End of - Headers==============================
Depending on the composition of the bug fossils, you might have some luck trying high-resolution CT scanning (microCT). I know of some microCT scanning successes of mantis fossils with high iron content compared to the surrounding rock matrix. Although if you are not seeing much average atomic number difference by BSE imaging, maybe CT would also not work well.
If you need more info on microCT of bugs, please don't hesitate to contact me offline.
Best regards,
Angela
Angela V. Klaus, Ph.D. Director, Microscopy and Imaging Facility American Museum of Natural History Central Park West and 79th Street New York, NY 10024 USA
Tel: 212-769-5977 Email: avklaus-at-amnh.org
-----Original Message----- X-from: rbeavers-at-mail.smu.edu [mailto:rbeavers-at-mail.smu.edu] Sent: Friday, November 18, 2005 12:52 PM To: avklaus-at-amnh.org
Group
l have been trying to look at some bug fossils in rock samples with SEM. In hand specimen these bugs stand out as dark areas against light tan rock material. In the SEM using backscatter they tend to fade into the background which seems to say there is not much organic material left. SEM secondary electron images do not yeild much information either.
Would like to hear any ideas on other techniques people have use to image such samples.
Thanks
Roy Beavers
Southern Methodist University Department of Geological Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 8, 20 -- From rbeavers-at-mail.smu.edu Fri Nov 18 11:51:30 2005 8, 20 -- Received: from s31xe5.systems.smu.edu (s31xe5.systems.smu.edu [129.119.70.74]) 8, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAIHpU86001271 8, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Nov 2005 11:51:30 -0600 8, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 8, 20 -- Content-class: urn:content-classes:message 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Type: text/plain; 8, 20 -- charset="iso-8859-1" 8, 20 -- Subject: Imaging bug fossils 8, 20 -- Date: Fri, 18 Nov 2005 11:51:28 -0600 8, 20 -- Message-ID: {3A9F6E299461A44483961A57175598A703402CAA-at-s31xe5.systems.smu.edu} 8, 20 -- X-MS-Has-Attach: 8, 20 -- X-MS-TNEF-Correlator: 8, 20 -- Thread-Topic: Imaging bug fossils 8, 20 -- thread-index: AcXsaLN/mflZ3DQtQ66cS0a1xut+XQ== 8, 20 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 8, 20 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAIHpU86001271 ==============================End of - Headers==============================
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Depending on the BSE detector you are using, you might not see much contrast. We had a 4-quadrant solid-state detector mounted coaxially with the beam. It does not show much topography when all four quadrants are summed together. That mode does show the best composition contrast, but I don't know how much compositional contrast you should expect for fossils in rock.
Are the fossilizing minerals the same as the host rock? If not, you should be able to get elemental contrast via an x-ray map image. BTW, you may need to experiment with the beam voltage depending on the thickness of your "bugs". A 20kV beam could be blasting all the way through them.
Still, I would remain hopeful. I know that optical contrast does not always translate into contrast in the EM, but often there is some way to tease out contrast in some imaging mode or another.
Warren Straszheim
-----Original Message----- X-from: rbeavers-at-mail.smu.edu [mailto:rbeavers-at-mail.smu.edu] Sent: Friday, November 18, 2005 11:53 AM To: wesaia-at-iastate.edu
Roy,
Two thoughts come to mind. First, if you have access to a low-kV SEM, I'd try imaging at 1 or 1.5 kV with secondaries. You'd more likely differentiate the fossil from the matrix this way. The other thought is: are this organic films? phosphatized? ... ? Meaning, have you tried etching the rocks with weak acetic acid? This often works to bring out fossils (or remove them from the matrix entirely). It might also be the worst possible thing to do, and should be tried first on a fossil that doesn't matter.
Phil
Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
Group
l have been trying to look at some bug fossils in rock samples with SEM. In hand specimen these bugs stand out as dark areas against light tan rock material. In the SEM using backscatter they tend to fade into the background which seems to say there is not much organic material left. SEM secondary electron images do not yeild much information either.
Would like to hear any ideas on other techniques people have use to image such samples.
Thanks
Roy Beavers
Southern Methodist University Department of Geological Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 8, 20 -- From rbeavers-at-mail.smu.edu Fri Nov 18 11:51:30 2005 8, 20 -- Received: from s31xe5.systems.smu.edu (s31xe5.systems.smu.edu [129.119.70.74]) 8, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAIHpU86001271 8, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Nov 2005 11:51:30 -0600 8, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 8, 20 -- Content-class: urn:content-classes:message 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Type: text/plain; 8, 20 -- charset="iso-8859-1" 8, 20 -- Subject: Imaging bug fossils 8, 20 -- Date: Fri, 18 Nov 2005 11:51:28 -0600 8, 20 -- Message-ID: {3A9F6E299461A44483961A57175598A703402CAA-at-s31xe5.systems.smu.edu} 8, 20 -- X-MS-Has-Attach: 8, 20 -- X-MS-TNEF-Correlator: 8, 20 -- Thread-Topic: Imaging bug fossils 8, 20 -- thread-index: AcXsaLN/mflZ3DQtQ66cS0a1xut+XQ== 8, 20 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 8, 20 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAIHpU86001271 ==============================End of - Headers==============================
==============================Original Headers============================== 14, 30 -- From oshel1pe-at-cmich.edu Fri Nov 18 14:15:01 2005 14, 30 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 14, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAIKF0qD029705 14, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Nov 2005 14:15:00 -0600 14, 30 -- Received: from egateb.central.cmich.local ([141.209.15.85]) 14, 30 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id jAIKvWwi021109 14, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Nov 2005 15:57:32 -0500 14, 30 -- Received: from cmail4.central.cmich.local ([141.209.15.84]) by egateb.central.cmich.local with Microsoft SMTPSVC(5.0.2195.6713); 14, 30 -- Fri, 18 Nov 2005 15:14:49 -0500 14, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 14, 30 -- Content-class: urn:content-classes:message 14, 30 -- MIME-Version: 1.0 14, 30 -- Content-Type: text/plain; 14, 30 -- charset="iso-8859-1" 14, 30 -- Subject: RE: [Microscopy] Imaging bug fossils 14, 30 -- Date: Fri, 18 Nov 2005 15:14:59 -0500 14, 30 -- Message-ID: {07BD45BB3EE8A0468C94776E7A0C660E3A305F-at-cmail4.central.cmich.local} 14, 30 -- X-MS-Has-Attach: 14, 30 -- X-MS-TNEF-Correlator: 14, 30 -- Thread-Topic: [Microscopy] Imaging bug fossils 14, 30 -- Thread-Index: AcXsaXmOcURDnkeeSOePoEmH3cefxwAEhf4q 14, 30 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 14, 30 -- To: {rbeavers-at-mail.smu.edu} 14, 30 -- Cc: {Microscopy-at-microscopy.com} 14, 30 -- X-OriginalArrivalTime: 18 Nov 2005 20:14:49.0657 (UTC) FILETIME=[BA5B4290:01C5EC7C] 14, 30 -- X-CanItPRO-Stream: default 14, 30 -- X-Spam-Score: -4 () L_EXCH_MF 14, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 14, 30 -- Content-Transfer-Encoding: 8bit 14, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAIKF0qD029705 ==============================End of - Headers==============================
What exactly are you trying to accomplish? If you want morphology, low KV SE should do it. BSE is just going to show difference in Z. Since the bug is or was organic, it ought not show much with BSE.
If you want to do morphology and save the specimen, perhaps doing a replica would work. Use replica resin and then sputter coat it and then SEM it. Invert the image and you should have excellent morphology results.
Another idea is CL.
gary g.
At 09:53 AM 11/18/2005, you wrote:
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==============================Original Headers============================== 10, 24 -- From gary-at-gaugler.com Fri Nov 18 14:36:02 2005 10, 24 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jAIKa251006350 10, 24 -- for {microscopy-at-microscopy.com} ; Fri, 18 Nov 2005 14:36:02 -0600 10, 24 -- Received: (qmail 19431 invoked from network); 18 Nov 2005 12:35:11 -0800 10, 24 -- Received: by simscan 1.1.0 ppid: 19418, pid: 19419, t: 3.1227s 10, 24 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1142 spam: 3.0.3 10, 24 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 24 -- by qsmtp2 with SMTP; 18 Nov 2005 12:35:08 -0800 10, 24 -- Message-Id: {6.2.3.4.2.20051118123138.0296c840-at-mail.calweb.com} 10, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 10, 24 -- Date: Fri, 18 Nov 2005 12:35:59 -0800 10, 24 -- To: rbeavers-at-mail.smu.edu 10, 24 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 24 -- Subject: Re: [Microscopy] Imaging bug fossils 10, 24 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 24 -- In-Reply-To: {200511181753.jAIHreVu004558-at-ns.microscopy.com} 10, 24 -- References: {200511181753.jAIHreVu004558-at-ns.microscopy.com} 10, 24 -- Mime-Version: 1.0 10, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 10, 24 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp2.surewest.net 10, 24 -- X-Spam-Level: 10, 24 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 10, 24 -- version=3.0.3 ==============================End of - Headers==============================
We've struggled all we can to to keep our Ilford photo processor working. It's time we entered the digital age. Can anyone suggest a TEM negative scanner? Ideally it would take a stack of negatives and feed them into the scanner and produce individual files, so if anyone can suggest a model that does this I'd be ecstatic. Otherwise, if you have a scanner that will automatically scan 12 or more negatives, producing individual files of each, please share your experience.
Many thanks,
doug
Douglas R. Keene Assistant Investigator Micro-Imaging Center Research Department Shriners Hospital for Children 3101 S.W. Sam Jackson Park Road Portland, Oregon 97239-3009 voice: 503-221-3434
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This Question was submitted to Ask-A-Microscopist by (jmcss-at-aol.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, November 19, 2005 at 06:46:02 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both jmcss-at-aol.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: jmcss-at-aol.com Name: Mary Hamelin
Organization: Country Day School
Education: 6-8th Grade Middle School
Location: Groton, MA
Title: plants
Question: What is the best way to help the students visualize stomata using a microscope? I have tried clear nail polish to make a relief of the leaf's exterior but didn't have much luck. Is there a way to make good quality, thin sections of plant material with little equipment? I'd like to help the students discover different types of plant cells. Also, what staining, if any would you recommend?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sarj0007-at-unf.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sarj0007-at-unf.edu Name: Jason Saredy
Organization: University of North Florida
Title-Subject: [Filtered] Joel 35-CF
Question: Hi. We are trying to get an old Joel-35CF SEM up and running from a long period of not being used. When turned on there is smoke coming out of the inside of the voltage splitter where, according to the schematics, the transformers are. The smoke doesnít start to come out until about 30-40 seconds after being turned on and its pulling at least 5 amps from a circuit that should only be pulling 3 amps. Anyone knows whether this is an easy to replace part or should we replace the whole power supply. We are doing this out of pocket so prefer to not purchase a new one directly from Joel. If so does anyone have some parts lying around? Or a good website for finding this sort of information? Thanks
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Email: jbradley-at-igpp.ucllnl.org Name: John Bradley
Organization: Lawrence Livermore Laboratory
Title-Subject: [Filtered] SMTF - A Noran TN5500 program
Question: I am looking for a copy of the Noran program "SMTF" (Standardless Metallurgical Thin Film).
I have several hundred TEM/EDX spectra archived on 5.25 inch floppy discs. I also have access to a TN5500 with a 5.25 inch floppy disc drive. I need is a copy of SMTF on a 5.25 inch floppy disc.
I would also very much like to know how these files can be exported from the TN5500.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both watson-at-wi.mit.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: Hi I am looking for protocols for immunolabeling cultured cells using Iodine 125/silver enhancement techniques. I think this is a rather old fashion method, but I have a professor interested in it for a specific project. We would also be interested in the opinions of you experts as to the functionality of this technique.Do you prefer gold, or peroxidase, quantum dots ? Does the iodine 125 have any special features that make it a great choice? Any input would be great! thanks for your input Nicki Watson
Hi Mary, I am not an expert in this area but I can suggest a couple of things to try.
First, check out Project Micro, this has a ton of useful information about microscopes in education, for all levels. http://microscopy.org/ProjectMICRO/
Second, don't forget to see what you can see with a good hand lens, for example a jewler's loupe. This shows a great deal (though not usually cells) and is a great intermediate between the eye and the microscope. Lots of leaves (flowers, etc) make elaborate hairs and things, which look great even with modest magnification.
Third, remember that in many (most?) plants, stomata are on the abaxial side of the leaf (ie, towards the ground).
Fouth. A trick popular with stomatal researchers is to make an epidermal peel. You take a tweezers and grab near the midvein and carefully pull toward the leaf margin. You can often remove a flap of epidermis. Put that in some water on a slide under a coverslip and you should be able to see stomata even in brighfield. Supposedly Vicia faba (bean) is easy to peel. But note that for your purpose, even a small (few mm square) area should suffice. It might be fun for your class to try some different plants and see which peel better?
Fifth. If you do get good at peeling, you can even try some things to open or close the stomata. Add some CaCl2 to the water where the peels are, or sodium bicarbonate. See what happens. Manipulate the light environment of the plants before you make peels (keep some plants in the dark, others in bright light). This could be beyond what you have in mind, but I thought I'd mention it just in case.
Have fun
Hope this helps, Tobias Baskin
} } } Email: jmcss-at-aol.com } Name: Mary Hamelin } } Organization: Country Day School } } Education: 6-8th Grade Middle School } } Location: Groton, MA } } Title: plants } } Question: What is the best way to help the students visualize } stomata using a microscope? I have tried clear nail polish to make a } relief of the leaf's exterior but didn't have much luck. Is there a } way to make good quality, thin sections of plant material with } little equipment? I'd like to help the students discover different } types of plant cells. Also, what staining, if any would you } recommend? }
Hi everyone, I am trying to conjugate my colloidal gold particles (0.8 nm and 6 nm particle sizes) with fluoresceinated BSA. I have tried to perform extensive literature search for detailed protocols but most of what I found were either for specific proteins (like IgG or Protein A) or general recipes that don't really say much about what I should do.
I know that we have experts out there who have done such procedures (or others that may be very similar) in the past; any advice/information would be greatly appreciated.
Have a happy thanksgiving!
Sincerely, Carlo
---
Carlo Franco Bolivar Balane
Box 4058, 222 Church Street, or Wolfe Laboratory Wesleyan University Station Rm. 157, HA Laboratories Middletown, CT, 06459-4058 Wesleyan University phone: 1.860.759.2830 phone: 1.860.685.3275
==============================Original Headers============================== 8, 34 -- From cbalane-at-wesleyan.edu Sat Nov 19 13:35:25 2005 8, 34 -- Received: from post1.wesleyan.edu (post1.wesleyan.edu [129.133.6.131]) 8, 34 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAJJZPak021194 8, 34 -- for {microscopy-at-microscopy.com} ; Sat, 19 Nov 2005 13:35:25 -0600 8, 34 -- Received: from pony1.wesleyan.edu (pony1.wesleyan.edu [129.133.6.192]) 8, 34 -- by post1.wesleyan.edu (8.12.11/8.12.11) with ESMTP id jAJJZQoq001890 8, 34 -- for {microscopy-at-microscopy.com} ; Sat, 19 Nov 2005 14:35:26 -0500 8, 34 -- Received: from pony1.wesleyan.edu (pony1.wesleyan.edu [127.0.0.1]) 8, 34 -- by pony1.wesleyan.edu (8.12.11/8.12.11) with ESMTP id jAJJZNfi020896 8, 34 -- for {microscopy-at-microscopy.com} ; Sat, 19 Nov 2005 14:35:23 -0500 8, 34 -- Received: (from apache-at-localhost) 8, 34 -- by pony1.wesleyan.edu (8.12.11/8.12.11/Submit) id jAJJZNlR020894; 8, 34 -- Sat, 19 Nov 2005 14:35:23 -0500 8, 34 -- Received: from 129.133.124.241 8, 34 -- (SquirrelMail authenticated user cbalane); 8, 34 -- by webmail.wesleyan.edu with HTTP; 8, 34 -- Sat, 19 Nov 2005 14:35:23 -0500 (EST) 8, 34 -- Message-ID: {51788.129.133.124.241.1132428923.squirrel-at-129.133.124.241} 8, 34 -- In-Reply-To: {200511191646.jAJGkLcq017833-at-ns.microscopy.com} 8, 34 -- References: {200511191646.jAJGkLcq017833-at-ns.microscopy.com} 8, 34 -- Date: Sat, 19 Nov 2005 14:35:23 -0500 (EST) 8, 34 -- Subject: gold conjugation with fluorescent BSA 8, 34 -- From: "Carlo Franco Balane-Bolivar" {cbalane-at-wesleyan.edu} 8, 34 -- To: microscopy-at-microscopy.com 8, 34 -- User-Agent: SquirrelMail/1.4.3a-0.e3.1 8, 34 -- X-Mailer: SquirrelMail/1.4.3a-0.e3.1 8, 34 -- MIME-Version: 1.0 8, 34 -- Content-Type: text/plain;charset=iso-8859-1 8, 34 -- Content-Transfer-Encoding: 8bit 8, 34 -- X-Priority: 3 (Normal) 8, 34 -- Importance: Normal 8, 34 -- X-Wesleyan-MailScanner-Information: Please contact the ISP for more information 8, 34 -- X-Wesleyan-MailScanner: Found to be clean 8, 34 -- X-MailScanner-From: cbalane-at-wesleyan.edu ==============================End of - Headers==============================
Hi Doug, The $350-500 Epson and Canon flatbeds seem hard to beat for price and resolution, but don't have film autofeeders or multi-holders for 3.25 x 4" negatives; you must fit one negative at a time into the 4"x5" holder. I'm not sure jamproof autofeeders exist.
600 dpi scans will serve most workprint needs (600 scans 29 sec or less with new EPSON 4990 PRO, says Amazon). Keep the film! for selected re-scan when needing greater enlargement. My betters advise that it is better (more dynamic range/) to scan as a transparency and invert rather than as a negative. Make excellent workprints fast on standard paper on an HP 4100 LaserJet set for 150 lpi. (exceeds printer spec of 1200 dpi, I know, but it works).
For general info look at http://scantips.com/ .
For additional general info look over many comments and viewpoints at http://www.macintouch.com/scanners05.html (also parts 4 amd 6) Note Steve Pucci's comments on batch-feed film scanners under Aug 25, 2005 entries. Check out his preferred Canon 9950 scanner for its Customer Reviews comments at Amazon.com; then see also the Epson Perfection 4870 PRO Customer Reviews. The newest Epson, 4990 PRO, has no Amazon reviews yet, but see this and other scanners, including Microtek, reviewed at CNET: http://reviews.cnet.com/Epson_Perfection_4990_Pro/4514-3136_7-31320383.html (latter site also has reviews and customer comments for the other top scanners).
-mike reedy
At 7:20 PM -0600 11/18/05, DRK-at-SHCC.org wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rpowell-at-nanoprobes.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rpowell-at-nanoprobes.com Name: Richard D. Powell
Organization: Nanoprobes, Incorporated
Title-Subject: [Filtered] Re: [Microscopy] gold conjugation with fluorescent BSA
Question: Hello Carlo:
Most colloidal gold conjugation protocols are for antibodies or other targeting agents. BSA is used as a stabilizer in these reactions, but because conjugation of the targeting agent takes priority, the reaction conditions are usually optimized for conjugating to this rather than the BSA. However, you don't need to make many changes - the procedure is similar for most colloidal gold conjugations.
Optimum colloidal gold conjugation is usually done at a pH at or just above the pI of the protein you are conjugating. From a quick search, the pI of BSA is about 4.7, so a pH of about 5 to 5.2 would be good for conjugation. Usually you would dialyze the protein into a dilute buffer at this pH or deionized water; use a buffer that does not flocculate colloidal gold (for example 0.002 M citrate, pH 5). Adjust the pH of the colloidal gold sol to the same pH using dilute H3PO4; then add the minimum stabilizing amount of fluorescein-BSA to the gold (you can determine this from titration; a procedure is given in the link below), stir for 2 minutes, then add a further 10% of BSA and 0.1% carbowax (a special form of 20,000 MW polyethylene glycol), stir 10 - 15 minutes more.
A good procedure for antibodies is given in this link. To adapt for albumin, all you need to do is to change the pH for the titration and conjugation to one more suited to BSA (adjust pH with H3PO4 if you need to lower rather than raise it):
http://www.researchd.com/gold/gold8.htm
All glassware must be scrupulously clean. Glass and plastic containers and stirrers should be cleaned in aqua regia, thoroughly washed in deionized water, and siliconized.
Once you have prepared the conjugate, you should store at a pH several units away from the PI, as this helps stabilize the conjugate. Spin down and resuspend in 0.02 M Tris-HCl, pH 8.2 (the usual buffer for storing colloidal gold conjugates).
Before doing this, though, you should be aware that fluorescence will likely be completely quenched if you conjugate fluorescein-streptavidin to 6 nm gold - gold is a very efficient absorber for resonance energy transfer, and other mechanimsms may also be present that will quench the fluorescence even more than energy transfer alone. We have explained this, and Albrecht and co-workers have observed it in practice when making combined fluorescent and 6 nm gold-labeled antibodies:
(1) Powell, R. D.; Halsey, C. M. R., and Hainfeld, J. F.: Combined fluorescent and gold immunoprobes: Reagents and methods for correlative light and electron microscopy. Microsc. Res. Tech., 1998, 42, 2.
(2) Kandela, I. K.; Meyer, D. A.; Oshel, P. E.; Rosa-Molinar, E., and Albrecht, R. M.: Fluorescence Quenching by Colloidal Heavy Metals: Implications for Correlative Fluorescence and Electron Microscopy Studies. Microsc. Microanal., 9, (Suppl. 2: Proceedings); Piston, D.; Bruley, J.; Anderson, I. M.; Kotula, P.; Solorzano, G.; Lockley, A., and McKernan, S. (Eds.); Cambridge University Press, New York, NY, 2003, 1194CD.
Hope this is helpful,
Rick Powell
***************************************************************************************** Richard D. Powell www.nanoprobes.com NANOPROBES, Incorporated *****************************************************************************************
Hi everyone, I am trying to conjugate my colloidal gold particles (0.8 nm and 6 nm particle sizes) with fluoresceinated BSA. I have tried to perform extensive literature search for detailed protocols but most of what I found were either for specific proteins (like IgG or Protein A) or general recipes that don't really say much about what I should do.
I know that we have experts out there who have done such procedures (or others that may be very similar) in the past; any advice/information would be greatly appreciated.
Have a happy thanksgiving!
Sincerely, Carlo
---
Carlo Franco Bolivar Balane
Box 4058, 222 Church Street, or Wolfe Laboratory Wesleyan University Station Rm. 157, HA Laboratories Middletown, CT, 06459-4058 Wesleyan University phone: 1.860.759.2830 phone: 1.860.685.3275
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rpowell-at-nanoprobes.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rpowell-at-nanoprobes.com Name: Richard D. Powell
Organization: Nanoprobes, Incorporated
Title-Subject: [Filtered] Re: [Microscopy] gold conjugation with fluorescent BSA
Question: Hello Carlo:
Most colloidal gold conjugation protocols are for antibodies or other targeting agents. BSA is used as a stabilizer in these reactions, but because conjugation of the targeting agent takes priority, the reaction conditions are usually optimized for conjugating to this rather than the BSA. However, you don't need to make many changes - the procedure is similar for most colloidal gold conjugations.
Optimum colloidal gold conjugation is usually done at a pH at or just above the pI of the protein you are conjugating. From a quick search, the pI of BSA is about 4.7, so a pH of about 5 to 5.2 would be good for conjugation. Usually you would dialyze the protein into a dilute buffer at this pH or deionized water; use a buffer that does not flocculate colloidal gold (for example 0.002 M citrate, pH 5). Adjust the pH of the colloidal gold sol to the same pH using dilute H3PO4; then add the minimum stabilizing amount of fluorescein-BSA to the gold (you can determine this from titration; a procedure is given in the link below), stir for 2 minutes, then add a further 10% of BSA and 0.1% carbowax (a special form of 20,000 MW polyethylene glycol), stir 10 - 15 minutes more.
A good procedure for antibodies is given in this link. To adapt for albumin, all you need to do is to change the pH for the titration and conjugation to one more suited to BSA (adjust pH with H3PO4 if you need to lower rather than raise it):
http://www.researchd.com/gold/gold8.htm
All glassware must be scrupulously clean. Glass and plastic containers and stirrers should be cleaned in aqua regia, thoroughly washed in deionized water, and siliconized.
Once you have prepared the conjugate, you should store at a pH several units away from the PI, as this helps stabilize the conjugate. Spin down and resuspend in 0.02 M Tris-HCl, pH 8.2 (the usual buffer for storing colloidal gold conjugates).
Before doing this, though, you should be aware that fluorescence will likely be completely quenched if you conjugate fluorescein-streptavidin to 6 nm gold - gold is a very efficient absorber for resonance energy transfer, and other mechanimsms may also be present that will quench the fluorescence even more than energy transfer alone. We have explained this, and Albrecht and co-workers have observed it in practice when making combined fluorescent and 6 nm gold-labeled antibodies:
(1) Powell, R. D.; Halsey, C. M. R., and Hainfeld, J. F.: Combined fluorescent and gold immunoprobes: Reagents and methods for correlative light and electron microscopy. Microsc. Res. Tech., 1998, 42, 2.
(2) Kandela, I. K.; Meyer, D. A.; Oshel, P. E.; Rosa-Molinar, E., and Albrecht, R. M.: Fluorescence Quenching by Colloidal Heavy Metals: Implications for Correlative Fluorescence and Electron Microscopy Studies. Microsc. Microanal., 9, (Suppl. 2: Proceedings); Piston, D.; Bruley, J.; Anderson, I. M.; Kotula, P.; Solorzano, G.; Lockley, A., and McKernan, S. (Eds.); Cambridge University Press, New York, NY, 2003, 1194CD.
Hope this is helpful,
Rick Powell
***************************************************************************************** Richard D. Powell www.nanoprobes.com NANOPROBES, Incorporated *****************************************************************************************
Hi everyone, I am trying to conjugate my colloidal gold particles (0.8 nm and 6 nm particle sizes) with fluoresceinated BSA. I have tried to perform extensive literature search for detailed protocols but most of what I found were either for specific proteins (like IgG or Protein A) or general recipes that don't really say much about what I should do.
I know that we have experts out there who have done such procedures (or others that may be very similar) in the past; any advice/information would be greatly appreciated.
Have a happy thanksgiving!
Sincerely, Carlo
---
Carlo Franco Bolivar Balane
Box 4058, 222 Church Street, or Wolfe Laboratory Wesleyan University Station Rm. 157, HA Laboratories Middletown, CT, 06459-4058 Wesleyan University phone: 1.860.759.2830 phone: 1.860.685.3275
Gold particles (and in fact, most clean surfaces of a noble metal, will bind to any type of protein. The binding is apparently not covalent but it is very tight. If you follow a procedure for binding IgG or Protein A, but what you supply is only BSA, the BSA will bind. BSA does not adhere to surfaces as tightly as many other proteins. There is a lot of literature on the adhesion, look particularly for articles by Andrade who was at University of Utah. If you cross-search adhesion and the professor's name, you should be able to find them. Carol
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-- __ Carol A. Heckman, Ph.D. Professor of Biological Sciences Director, Center for Microscopy & Microanalysis Bowling Green State University, Bowling Green, OH 43403 fax: (419) 372-2024 email: heckman-at-bgnet.bgsu.edu http://www.bgsu.edu/departments/biology/facilities/MnM ___________________________________________________________________________
==============================Original Headers============================== 5, 17 -- From heckman-at-bgnet.bgsu.edu Sun Nov 20 14:12:38 2005 5, 17 -- Received: from smtp01.bgsu.edu (smtp01.bgsu.edu [129.1.5.17]) 5, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAKKCcOL015083 5, 17 -- for {microscopy-at-microscopy.com} ; Sun, 20 Nov 2005 14:12:38 -0600 5, 17 -- Received: from [129.1.85.81] (dhcp-85-81.bgsu.edu [129.1.85.81]) 5, 17 -- by smtp01.bgsu.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id jAKKCZLw000741 5, 17 -- for {microscopy-at-microscopy.com} ; Sun, 20 Nov 2005 15:12:37 -0500 (EST) 5, 17 -- Mime-Version: 1.0 5, 17 -- X-Sender: heckman-at-mailstore.bgsu.edu (Unverified) 5, 17 -- Message-Id: {p04320401bfa6b2469476-at-[129.1.85.81]} 5, 17 -- In-Reply-To: {200511191936.jAJJaMmT022696-at-ns.microscopy.com} 5, 17 -- References: {200511191936.jAJJaMmT022696-at-ns.microscopy.com} 5, 17 -- Date: Sun, 20 Nov 2005 15:12:33 -0800 5, 17 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 5, 17 -- From: Carol Heckman {heckman-at-bgnet.bgsu.edu} 5, 17 -- Subject: Re: [Microscopy] gold conjugation with fluorescent BSA 5, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Dear Listers, I have been using HM20 Monostep (premixed) resin for freeze substitution for a couple of years, and have recently encountered problems with batch variability. Sarah Ellis of the Peter MacCallum Cancer Clinic in Melbourne has recently experienced similar problems. The main problem is incomplete or variable infiltration, resulting in polymerised resin pulling away from the edge of tissue blocks or cells, leaving gaps and making sections hard to cut and to view. In some cases there are gaps around larger organelles within the cells. Another batch frosted badly at -45 degrees C.
My question to listserver members is whether anyone else is having these variability problems. More importantly, does anyone have a solution?
Thanks, Rosey van Driel
Rosemary van Driel Electron Microscopy New and Emerging Zoonotic Diseases Australian Animal Health Laboratory CSIRO Livestock Industries Private Bag 24 Geelong Vic 3220 Australia
International Phone: +61 3 5227 5209 International Fax: +61 3 5227 5555 email: Rosey.VanDriel-at-csiro.au
==============================Original Headers============================== 7, 29 -- From Rosey.VanDriel-at-csiro.au Sun Nov 20 16:22:36 2005 7, 29 -- Received: from vic-ironport-ext-out1.csiro.au (vic-ironport-ext-out1.csiro.au [150.229.64.37]) 7, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAKMMZpI025741 7, 29 -- for {Microscopy-at-microscopy.com} ; Sun, 20 Nov 2005 16:22:36 -0600 7, 29 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 7, 29 -- by vic-ironport-ext-out1.csiro.au with ESMTP; 21 Nov 2005 09:22:29 +1100 7, 29 -- X-IronPort-AV: i="3.97,355,1125842400"; 7, 29 -- d="scan'208"; a="58831565:sNHT7698219152" 7, 29 -- Received: from exvicn2-mel.nexus.csiro.au ([138.194.3.62]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 7, 29 -- Mon, 21 Nov 2005 09:22:29 +1100 7, 29 -- Received: from EXVIC4-GEX.nexus.csiro.au ([138.194.208.10]) by exvicn2-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 7, 29 -- Mon, 21 Nov 2005 09:22:29 +1100 7, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 7, 29 -- content-class: urn:content-classes:message 7, 29 -- MIME-Version: 1.0 7, 29 -- Content-Type: text/plain; 7, 29 -- charset="us-ascii" 7, 29 -- Subject: TEM: HM20 problems 7, 29 -- Date: Mon, 21 Nov 2005 09:22:28 +1100 7, 29 -- Message-ID: {8BE5C4878E8768478169A50D38516052019825-at-exvic4-gex.nexus.csiro.au} 7, 29 -- X-MS-Has-Attach: 7, 29 -- X-MS-TNEF-Correlator: 7, 29 -- Thread-Topic: TEM: HM20 problems 7, 29 -- Thread-Index: AcXuIOROcUStu1R7RM6t9nTe95eHJw== 7, 29 -- From: {Rosey.VanDriel-at-csiro.au} 7, 29 -- To: {Microscopy-at-microscopy.com} 7, 29 -- X-OriginalArrivalTime: 20 Nov 2005 22:22:29.0380 (UTC) FILETIME=[E4BBBC40:01C5EE20] 7, 29 -- Content-Transfer-Encoding: 8bit 7, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAKMMZpI025741 ==============================End of - Headers==============================
I'm afraid that such a scanner would be very expensive. To scan the full frame of a 3.25x4 inch EM negative you will need a 4x5 film scanner, not an inexpensive item. I suggest two alternatives. 1. A good flatbed scanner will be 1/10 the cost of a 4x5 film scanner. 2. Put your negatives on a light box and photograph them with a digital camera. Most will focus very close, then invert the image (negative to postitive) in PhotoShop.
Geoff
DRK-at-SHCC.org wrote:
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-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 8, 31 -- From mcauliff-at-umdnj.edu Mon Nov 21 10:32:48 2005 8, 31 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126]) 8, 31 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jALGWlMG022975 8, 31 -- for {microscopy-at-msa.microscopy.com} ; Mon, 21 Nov 2005 10:32:48 -0600 8, 31 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1]) 8, 31 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 05D52230072 8, 31 -- for {microscopy-at-msa.microscopy.com} ; Mon, 21 Nov 2005 11:32:45 -0500 (EST) 8, 31 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 8, 31 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 6AC5DEC0BC 8, 31 -- for {microscopy-at-msa.microscopy.com} ; Mon, 21 Nov 2005 11:32:43 -0500 (EST) 8, 31 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 8, 31 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 8, 31 -- id {0IQB00101BDU3W-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 8, 31 -- for microscopy-at-msa.microscopy.com; Mon, 21 Nov 2005 11:32:41 -0500 (EST) 8, 31 -- Received: from [127.0.0.1] ([10.138.2.240]) 8, 31 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 8, 31 -- 2004)) with ESMTP id {0IQB0066KBVOGJ-at-Polaris.umdnj.edu} ; Mon, 8, 31 -- 21 Nov 2005 11:16:40 -0500 (EST) 8, 31 -- Date: Mon, 21 Nov 2005 11:16:26 -0500 8, 31 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 8, 31 -- Subject: Re: [Microscopy] TEM negative scanner 8, 31 -- In-reply-to: {200511190117.jAJ1H9uS020180-at-ns.microscopy.com} 8, 31 -- To: DRK-at-SHCC.org, MicroscopyListserver {microscopy-at-msa.microscopy.com} 8, 31 -- Message-id: {4381F2DA.30405-at-umdnj.edu} 8, 31 -- MIME-version: 1.0 8, 31 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 8, 31 -- Content-transfer-encoding: 7BIT 8, 31 -- X-Accept-Language: en-us, en 8, 31 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 8, 31 -- Gecko/20040804 Netscape/7.2 (ax) 8, 31 -- References: {200511190117.jAJ1H9uS020180-at-ns.microscopy.com} ==============================End of - Headers==============================
Dear listers, It would seem that our problems with Monostep HM20 may be due to aged or contaminated resin.
The Monostep was supplied by a company which had its own labels on otherwise unlabelled bottles. I have since learnt that the product is shipped from the manufacturer with a lot number and expiry date. The expiry dates are not on the product I received, which suggests there may have been some rebottling or aging of the product. Interestingly, the manufacturer was unable to trace the lot numbers printed on the supplier's labels. The supplier refuses to entertain the thought that a problem exists with the resin we recieved.
I have also discovered that resins ( and I don't know what else) can go through 3 resellers before they reach us in our labs. So buyer beware!
I have traced a more direct source of supply, so I hope that the problem is solved.
Thanks especially to the US commercial list subsciber who spared no effort in helping track down the likely sources of the problem, I'll get Alex to buy you a beer in Sydney in February. Thanks also to the other supply companies in Australia who helped. I'd like to name the helpful ones, but I don't think it's allowed.
Rosey
Rosemary van Driel Electron Microscopy New and Emerging Zoonotic Diseases Australian Animal Health Laboratory CSIRO Livestock Industries Private Bag 24 Geelong Vic 3220 Australia
International Phone: +61 3 5227 5209 International Fax: +61 3 5227 5555 email: Rosey.VanDriel-at-csiro.au
==============================Original Headers============================== 11, 29 -- From Rosey.VanDriel-at-csiro.au Mon Nov 21 22:34:39 2005 11, 29 -- Received: from vic-ironport-ext-out3.csiro.au (vic-ironport-ext-out3.csiro.au [150.229.64.39]) 11, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAM4YbJN021270 11, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Nov 2005 22:34:38 -0600 11, 29 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 11, 29 -- by vic-ironport-ext-out3.csiro.au with ESMTP; 22 Nov 2005 15:34:36 +1100 11, 29 -- X-IronPort-AV: i="3.97,359,1125842400"; 11, 29 -- d="scan'208"; a="58992765:sNHT23588968" 11, 29 -- Received: from exvicn2-mel.nexus.csiro.au ([138.194.3.62]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 11, 29 -- Tue, 22 Nov 2005 15:34:36 +1100 11, 29 -- Received: from EXVIC4-GEX.nexus.csiro.au ([138.194.208.10]) by exvicn2-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 11, 29 -- Tue, 22 Nov 2005 15:34:36 +1100 11, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 11, 29 -- content-class: urn:content-classes:message 11, 29 -- MIME-Version: 1.0 11, 29 -- Content-Type: text/plain; 11, 29 -- charset="us-ascii" 11, 29 -- Subject: TEM: HM20 problems: summary 11, 29 -- Date: Tue, 22 Nov 2005 15:34:35 +1100 11, 29 -- Message-ID: {8BE5C4878E8768478169A50D3851605201A5CE-at-exvic4-gex.nexus.csiro.au} 11, 29 -- X-MS-Has-Attach: 11, 29 -- X-MS-TNEF-Correlator: 11, 29 -- Thread-Topic: TEM: HM20 problems: summary 11, 29 -- Thread-Index: AcXvHgrWv97HLNocTh2DPG6dWWQjeA== 11, 29 -- From: {Rosey.VanDriel-at-csiro.au} 11, 29 -- To: {Microscopy-at-microscopy.com} 11, 29 -- X-OriginalArrivalTime: 22 Nov 2005 04:34:36.0216 (UTC) FILETIME=[0AFA4B80:01C5EF1E] 11, 29 -- Content-Transfer-Encoding: 8bit 11, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAM4YbJN021270 ==============================End of - Headers==============================
FOCUS ON MICROSCOPY 2006, Perth, Australia, April 9-12, 2006 19th International Conference on 3D Image Processing in Microscopy 18th International Conference on Confocal Microscopy
2nd Announcement:
Dear Colleagues,
Abstracts for oral and poster presentations can now be submitted preferably through the conference website: http://www.focusonmicroscopy.org where also the conference registration has opened and hotel information is available.
Deadline for abstract submission: Jan. 9, 2006. Note: this is earlier than in previous conferences!
The program will start on Sunday April 9, around 18 hours with an opening symposium followed by a welcome reception.
After the successful FOM2005 conference in Jena, the next conference Focus on Microscopy 2006 will take place in Perth, Western Australia, April 9-12, 2006. FOM2006 is the next in a series of unique interdisciplinary meetings on advanced multidimensional light microscopy and image processing. The conference will be hosted by the University of Western Australia in Perth and held in the beautiful Esplanade Hotel in the historic waterfront suburb of Perth, Fremantle.
Focus on Microscopy 2006 is the continuation of a conference series presenting the latest innovations in optical microscopy and its applications in biology, medicine, material science, and information storage. 3D optical imaging and related theory are traditional subjects for the conference. The conference series is also known for covering the rapid development of advanced fluorescence labeling techniques for the confocal andmulti-photon 3D imaging of -live- biological specimens. This year, in addition, special attention will be given to imaging in thick tissues.
Abstracts for contributions are invited and can now be submitted through the website:
www.FocusOnMicroscopy.org
where further information on the present and previous FOM conferences can be found.
Important dates:
Deadline for the submission of abstracts: January 9, 2006 Draft program available on the web: January 23, 2006 at website www.FocusOnMicroscopy.org Deadline for early registration: February 20, 2006
Welcoming you to beautiful Perth for the FOM2006 conference and exhibition.
On behalf of the organizing committee,
David Sampson, University of Western Australia, Perth, Australia
Fred Brakenhoff Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands
E-mail: info2006-at-FocusOnMicroscopy.org
Web: www.FocusOnMicroscopy.org
==============================Original Headers============================== 22, 32 -- From brakenho-at-science.uva.nl Tue Nov 22 09:22:02 2005 22, 32 -- Received: from imap.science.uva.nl (imap.science.uva.nl [146.50.4.51]) 22, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAMFM28g010604 22, 32 -- for {microscopy-at-msa.microscopy.com} ; Tue, 22 Nov 2005 09:22:02 -0600 22, 32 -- Received: from webmail.science.uva.nl [146.50.4.91] 22, 32 -- by imap.science.uva.nl with ESMTP (sendmail 8.11.6p2/config 11.35). 22, 32 -- id jAMFM0523064; Tue, 22 Nov 2005 16:22:00 +0100 22, 32 -- Received: from localhost 22, 32 -- by webmail.science.uva.nl (sendmail 8.11.6/config 11.35). 22, 32 -- id jAMFLxg22896; Tue, 22 Nov 2005 16:21:59 +0100 22, 32 -- X-Organisation: Faculty of Science, University of Amsterdam, The Netherlands 22, 32 -- X-URL: http://www.science.uva.nl/ 22, 32 -- Received: from localhost ([127.0.0.1]) 22, 32 -- (SquirrelMail authenticated user brakenho); 22, 32 -- by webmail.science.uva.nl with HTTP; 22, 32 -- Tue, 22 Nov 2005 16:21:59 +0100 (CET) 22, 32 -- Message-ID: {51507.62.194.19.126.1132672919.squirrel-at-62.194.19.126} 22, 32 -- Date: Tue, 22 Nov 2005 16:21:59 +0100 (CET) 22, 32 -- Subject: FOM2006, Abstracts, Registration, Perth, Australia, April 9-12, 22, 32 -- 2006, Focus on Microscopy conference. 22, 32 -- From: "Fred Brakenhoff" {brakenho-at-science.uva.nl} 22, 32 -- To: microscopy-at-msa.microscopy.com 22, 32 -- User-Agent: SquirrelMail/1.4.3a 22, 32 -- X-Mailer: SquirrelMail/1.4.3a 22, 32 -- MIME-Version: 1.0 22, 32 -- Content-Type: text/plain;charset=iso-8859-1 22, 32 -- Content-Transfer-Encoding: 8bit 22, 32 -- X-Priority: 3 (Normal) 22, 32 -- Importance: Normal 22, 32 -- References: 22, 32 -- In-Reply-To: 22, 32 -- X-Virus-Scanned: by amavisd-new ==============================End of - Headers==============================
Cryo system for SEM, Oxford CT 1500B, bought in 1996, in excellent condition, used only three times, will be sold for a very reasonable price. A good opportunity to try the cryo technique, or to get spare parts for an existing system. Don't hesitate to ask for more information!
****************************** Kerstin Brismar B.Sc., Research engineer, Photographer Dept. of Crop Science SLU (Swedish University of Agricultural Sciences) P.O. Box 44 (Delivery: Växtskyddsvägen 1) SE-230 53 Alnarp, Sweden Phone: +46 40 41 55 05 Fax: +46 40 41 55 19 E-mail: Kerstin.Brismar-at-vv.slu.se ******************************
==============================Original Headers============================== 5, 17 -- From Kerstin.Brismar-at-vv.slu.se Tue Nov 22 10:09:05 2005 5, 17 -- Received: from alnus.slu.se (alnus.slu.se [194.47.49.5]) 5, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAMG94Lf020049 5, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 22 Nov 2005 10:09:04 -0600 5, 17 -- Received: from vv-238.vv.slu.se (vv-238.vv.slu.se [194.47.228.116]) 5, 17 -- by alnus.slu.se (8.12.11/8.12.11) with ESMTP id jAMG93em022752 5, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 22 Nov 2005 17:09:03 +0100 (CET) 5, 17 -- Message-Id: {6.2.5.6.0.20051122165319.01ca7918-at-vv.slu.se} 5, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.5.6 5, 17 -- Date: Tue, 22 Nov 2005 17:09:03 +0100 5, 17 -- To: Microscopy-at-Microscopy.Com 5, 17 -- From: Kerstin Brismar {Kerstin.Brismar-at-vv.slu.se} 5, 17 -- Subject: SEM - Cryo system for sale 5, 17 -- Mime-Version: 1.0 5, 17 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 5, 17 -- Content-Transfer-Encoding: 8bit 5, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAMG94Lf020049 ==============================End of - Headers==============================
Michael- I checked your question with my Source, because I remembered only that I had long shared your viewpoint, and that he had changed my mind, but how....? So he reminded me . (The "they" he says set a smaller DR aperture for negative scans than for positive transparency scans are not Kodak, but scanner-makers in general, who rely on Kodak's loooong experience):
"I know it is counterintuitive but it is true and can be easily tested. In their haste to make scanning "better" they make and assumption based on loooong experience. Kodak discovered that most negatives have a transmission of 54% +- 5 %. They therefore set a smaller aperture in the dynamic range to get a better scan. The problem is that scientific imaging has a much wider need. Think brightfield microscopy with 98% transmission or darkfield with 2%. Because positive transparencies can have dark and light background they open up the dynamic range to get better scans. In most scanners scanning as a positive transparency and inverting will give you a much better histogram than scanning as a negative.
Epson is ONLY scanner that I recommend; this is because the actual calibrated resolution (I calibrated it myself) is 2000 dpi (4800dpi claimed). This is half the possible resolution of film, that can only be beaten with a 15,000 dpi drum scanner.
Our solution to a lack of film feeding is to put a scanner on every desk. Scanning can be done while doing email/reading/ etc"
My own thought is-- if there were no such difference, why would scanner controls continue to offer both options? ---- given that "invert" has been available with a keystroke for some time, at least since PhotoShop 2.0. I can think of possible simple tests, but I've yet to perform any.
-mike reedy-
} Mike Reedy writes ... } } } } [...] } } My betters advise that it is better (more dynamic range/) to } } scan as a transparency and invert rather than as a negative. } } [...] } } I don't believe this is correct. How is better DR related to inverting the } grayscale? } } genuinely :o) } michael shaffer } } SEM/MLA Laboratory Coordinator } (709) 737-6799 (ofc) } (709) 737-6790 (lab) } (709) 737-6193 (FAX) } {www.mun.ca/creait/maf/} } } Inco Centre } c/o Memorial University } 230 Elizabeth Avenue } P.O. Box 4200 } St. John's, NL A1C 5S7
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
As you approach the resolution of film what problems do you see with scanning the image from a random pattern of grain to the regular patter of pixels. It obvious that straight line at angles other than the XY pattern of the pixels are a problem but there are not that many straight lines in most images.
With large pixels and small grain there is a loss of contrast does that hold up as the pixel and grain size converge? As we are one or two doublings of scanning resolution away from the point that digital is as good or better than film the differences become more interesting.
With the drum scanners we have today one could scan in Kodak Tri X shot at ISO 800 developed in HC 110 dilution B and be able to over sample the grain on the negative for experemanal work. I think there are some photo journalist who still shoot this combination. It has a unique look on news print that some like.
I don't think there is anything standing in the way of higher resolution drum scanners but the size of the image being 4 time larger for every doubling in resolution and the computer power increasing by the square of the time it takes to do operations. Combine that with the cost of making a scanner to those tolerances and there is probably not a market for it.
Gordon Gordon Couger
I collect links on information related to light microscopes. www.couger.com/microscope/links/gclinks.html Please forward anything you think might be useful to others. Microscope Documentation is at www.science-info.org
mike.reedy-at-cellbio.duke.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Michael- } I checked your question with my Source, because I remembered only } that I had long shared your viewpoint, and that he had changed my } mind, but how....? So he reminded me . (The "they" he says set a } smaller DR aperture for negative scans than for positive transparency } scans are not Kodak, but scanner-makers in general, who rely on } Kodak's loooong experience): } } "I know it is counterintuitive but it is true and can be easily } tested. In their haste to make scanning "better" they make and } assumption based on loooong experience. Kodak discovered that most } negatives have a transmission of 54% +- 5 %. They therefore set a } smaller aperture in the dynamic range to get a better scan. The } problem is that scientific imaging has a much wider need. Think } brightfield microscopy with 98% transmission or darkfield with 2%. } Because positive transparencies can have dark and light background } they open up the dynamic range to get better scans. In most scanners } scanning as a positive transparency and inverting will give you a } much better histogram than scanning as a negative. } } Epson is ONLY scanner that I recommend; this is because the actual } calibrated resolution (I calibrated it myself) is 2000 dpi (4800dpi } claimed). This is half the possible resolution of film, that can } only be beaten with a 15,000 dpi drum scanner. } } Our solution to a lack of film feeding is to put a scanner on every } desk. Scanning can be done while doing email/reading/ etc" } } My own thought is-- if there were no such difference, why would } scanner controls continue to offer both options? ---- given that } "invert" has been available with a keystroke for some time, at least } since PhotoShop 2.0. I can think of possible simple tests, but I've } yet to perform any. } } -mike reedy- } } } } } Mike Reedy writes ... } } } } } } } } } [...] } } } My betters advise that it is better (more dynamic range/) to } } } scan as a transparency and invert rather than as a negative. } } } [...] } } } } I don't believe this is correct. How is better DR related to inverting the } } grayscale? } } } } genuinely :o) } } michael shaffer } } } } SEM/MLA Laboratory Coordinator } } (709) 737-6799 (ofc) } } (709) 737-6790 (lab) } } (709) 737-6193 (FAX) } } {www.mun.ca/creait/maf/} } } } } Inco Centre } } c/o Memorial University } } 230 Elizabeth Avenue } } P.O. Box 4200 } } St. John's, NL A1C 5S7 } } }
==============================Original Headers============================== 9, 21 -- From gcc-at-couger.com Tue Nov 22 19:01:47 2005 9, 21 -- Received: from centrmmtao04.cox.net (centrmmtao04.cox.net [70.168.83.80]) 9, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAN11lcJ013776 9, 21 -- for {microscopy-at-msa.microscopy.com} ; Tue, 22 Nov 2005 19:01:47 -0600 9, 21 -- Received: from [127.0.0.1] (really [68.12.242.13]) by centrmmtao04.cox.net 9, 21 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 9, 21 -- id {20051123010026.STSU8318.centrmmtao04.cox.net-at-[127.0.0.1]} ; 9, 21 -- Tue, 22 Nov 2005 20:00:26 -0500 9, 21 -- Message-ID: {4383BF83.6040108-at-couger.com} 9, 21 -- Date: Tue, 22 Nov 2005 19:01:55 -0600 9, 21 -- From: Gordon Couger {gcc-at-couger.com} 9, 21 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 9, 21 -- X-Accept-Language: en-us, en 9, 21 -- MIME-Version: 1.0 9, 21 -- To: mike.reedy-at-cellbio.duke.edu, 9, 21 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} 9, 21 -- Subject: Re: [Microscopy] TEM negative scanner 9, 21 -- References: {200511222224.jAMMOoPo007503-at-ns.microscopy.com} 9, 21 -- In-Reply-To: {200511222224.jAMMOoPo007503-at-ns.microscopy.com} 9, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: psneeley-at-xmission.com Name: Steve Neeley
Title-Subject: [Filtered] Looking for an AO Spencer Series 4 Microscope Reference Manual
Question: I have an AO Spencer Series 4 Microscope and need the Reference Manual. I have the Catalog, the Phase Catalog, and the Phase manual, but I need the actual reference manual that would have been provided for the scope by AO Spencer. These scopes were made in the 1950's and are blue/gray in color (Earlier scopes were the black Spencers and AO Spencers, later scopes were the Gray Series 10, etc.)
These microscope were heavily used at Colleges and Universities (many are still on the shelves, in labs, and in storage at least) and I'm hoping that someone has a copy of the manual.
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Email: pekysar-at-ucdavis.edu Name: Pat Kysar
Organization: University of California, Davis, School of Medicine,Pathology
Title-Subject: [Filtered] Identifying motor endplates in thick sections
Question: Hi all, We have a pathologist who is conducting a study which necessitates the need to locate motor endplates in resin embedded muscle tissue. He would like to sucessfully locate them in thick sections so we can accurately cut thin sections which includes the area of interest. Does anyone have a protocol for staining the endplates on the thick sections (epoxy resin embedded) to facilitate a more accurate identification of these structures? The pathologist has tried finding the areas on Methylene blue/Azure B stained sections with a very low rate of success and desires a more accurate method. Any help would be appreciated. Thanks,
Pat Kysar EM Lab University of California, Davis School of Medicine, Pathology 1 Shields Davis, CA 95616 pekysar-at-ucdavis.edu
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==============================Original Headers============================== 11, 25 -- From gary-at-gaugler.com Tue Nov 22 23:21:45 2005 11, 25 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jAN5Lj6M019144 11, 25 -- for {microscopy-at-microscopy.com} ; Tue, 22 Nov 2005 23:21:45 -0600 11, 25 -- Received: (qmail 8228 invoked from network); 22 Nov 2005 21:20:53 -0800 11, 25 -- Received: by simscan 1.1.0 ppid: 8207, pid: 8208, t: 5.3639s 11, 25 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1095 spam: 3.0.3 11, 25 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 25 -- by qsmtp1 with SMTP; 22 Nov 2005 21:20:48 -0800 11, 25 -- Message-Id: {6.2.3.4.2.20051122211838.029729a8-at-mail.calweb.com} 11, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 11, 25 -- Date: Tue, 22 Nov 2005 21:21:39 -0800 11, 25 -- To: gcc-at-couger.com 11, 25 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 25 -- Subject: Re: [Microscopy] Re: TEM negative scanner 11, 25 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 25 -- In-Reply-To: {200511230103.jAN13sbO017050-at-ns.microscopy.com} 11, 25 -- References: {200511230103.jAN13sbO017050-at-ns.microscopy.com} 11, 25 -- Mime-Version: 1.0 11, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 11, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on 11, 25 -- qsmtp1.mc.surewest.net 11, 25 -- X-Spam-Level: 11, 25 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 11, 25 -- version=3.0.3 ==============================End of - Headers==============================
Depends on your objectives, I suspect. If your objective is optimum combination of quality and versatility at the lowest cost then you can't go past Epson Photo scanners. Epson 4870 Photo can generate embarassingly large files from an EM negative which contain more detail than you can see on an 8"x10" photograph, and the quality of scans of 35mm transparencies can be good, more than adequate for 90% of work in scientific publishing. Having said that, photo buffs and EM people who are looking for highest fidelity will quickly notice limitations. DMax of 3.8 on 4870 (4.0 on 4990) looks good, but in practice flare from the uncoated glass platen compromises this performance. Images with extreme contrast (e.g. hard black areas against pure white) show visible and objectionable bleeding of light and colour into the dark areas. I find illumination is not uniform, so that large images with large areas of uniform density reproduce with density variation that is hard to correct. The 4870 is not really capable of resolving the grain of my transparencies, and introduces a characteristic artefactual speckling or peppering of pixels correlated with high-frequency information that can be objectionable when images are enlarged to display sizes. For this reason, I regard many of my scans of slides as adequate for printing A4, but below par for archiving. If these things will worry you, then you should probably look at a dedicated film scanner such as one of the Nikon Coolscans. They cost between 2 and 10 times the price, but you get what you pay for.
Chris
----- Original Message ----- X-from: {gary-at-gaugler.com} To: {c.jeffree-at-ed.ac.uk} Sent: Wednesday, November 23, 2005 5:22 AM
==============================Original Headers============================== 6, 24 -- From c.jeffree-at-ed.ac.uk Wed Nov 23 02:47:48 2005 6, 24 -- Received: from smtp808.mail.ukl.yahoo.com (smtp808.mail.ukl.yahoo.com [217.12.12.198]) 6, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jAN8llaU031722 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 23 Nov 2005 02:47:48 -0600 6, 24 -- Received: (qmail 73416 invoked from network); 23 Nov 2005 08:47:47 -0000 6, 24 -- Received: from unknown (HELO CARBERRY) (c.e.jeffree-at-81.158.32.213 with login) 6, 24 -- by smtp808.mail.ukl.yahoo.com with SMTP; 23 Nov 2005 08:47:46 -0000 6, 24 -- Message-ID: {000b01c5f00a$959e83e0$0100a8c0-at-CARBERRY} 6, 24 -- From: "Chris Jeffree" {c.jeffree-at-ed.ac.uk} 6, 24 -- To: {gary-at-gaugler.com} 6, 24 -- Cc: {microscopy-at-microscopy.com} 6, 24 -- References: {200511230522.jAN5M9iw019713-at-ns.microscopy.com} 6, 24 -- Subject: Re: [Microscopy] TEM negative scanner 6, 24 -- Date: Wed, 23 Nov 2005 08:47:49 -0000 6, 24 -- MIME-Version: 1.0 6, 24 -- Content-Type: text/plain; 6, 24 -- format=flowed; 6, 24 -- charset="iso-8859-1"; 6, 24 -- reply-type=original 6, 24 -- Content-Transfer-Encoding: 7bit 6, 24 -- X-Priority: 3 6, 24 -- X-MSMail-Priority: Normal 6, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 6, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
As the chat on scanners is continuing I'll add my thoughts.
We use an 'old' semi-pro Agfa flatbed scanner that cost five thousand pounds in its day - which runs on an Apple, as a Mac person bought it and most hi-end graphics were then MAC orientated. Unfortunately Agfa, Heidelberg and Fuji seem to have abandoned the pro scanner market, but the hi-res scanning of many large negatives manually wasn't a problem for us, as the scanner captures the images quite quickly and often we didn't require full resolution for subsequent image analysis - in fact most time is spent getting the images from Apple to IBM PC's. Apparently the cost of buying a digital camera add-on for our units TEM would be around £70k with 2k x 2k pixels and around £20k for 1k x 1k pixels (although our EM dept. has just decided to go down the £70k route and dispense with film completely).
Creo is one of the few companies that I know that still make this type of flat bed pro scanner, but I've no experience of their products. A scanner that can take lots of 4x5" negatives across the whole of its scan area and scan at 5000 dpi used to cost £10k +, and I have to admit I'm quite sure what, if anything, has replaced them. As we have the original negative anyway, 1600 dpi or less is fine for our digitising (mostly for subsequent image analysis or incorporation into documents). Our scanner is naturally used for slides, film, photo and paper copying as well.
You can still buy a few cheap sub £200 scanners with a light bulb area larger than 35mm for transpancies, but all the best cheap ones seem fixed to 35mm slides/negatives. As mentioned by others, probably something like the £750 Epson Expression 1680 Pro with its A4 transparency adapter would suite for manual scanning - the A4 Transparency Unit "provides the capability to scan multiple positive & negative films of up to 5"x4" in size" (the 1680 has 1600 x 3200dpi optical resolution). However we haven't got one to say whether it is as good as our old Agfa (it's certainly a lot cheaper but has similar specs). Things like hardware Digital ICE are probably less useful as our negatives are in good condition (although it works well on my home Benq Scanwit 2740S slide scanner that's used to scan very old colour slides of the family). Negative/slide scanners do seem to have real problems with grain size on my recent colour ASA 400 [and above] negatives generating optical effects, but fortunately that isn't a problem we see with our TEM negatives or my old Kodak/Agfa/Perutz 50 to 100 ASA slides from the 50s-70's.
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
Tel: 020 7608 4050 Fax: 020 7608 4034 email: keith.morris-at-ucl.ac.uk ----- Original Message ----- } DRK-at-SHCC.org wrote: } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 9, 28 -- From keith.morris-at-ucl.ac.uk Wed Nov 23 03:43:39 2005 9, 28 -- Received: from vscani-e2.ucl.ac.uk (vscani-e2.ucl.ac.uk [144.82.108.34]) 9, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAN9hcQ8008943 9, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Nov 2005 03:43:38 -0600 9, 28 -- Received: from cell23.ioo.ucl.ac.uk ([144.82.134.123] helo=keithhigrade) 9, 28 -- by vscani-e.ucl.ac.uk with smtp (Exim 4.51) 9, 28 -- id 1EerA6-0001jL-Qj 9, 28 -- for Microscopy-at-microscopy.com; Wed, 23 Nov 2005 09:43:34 +0000 9, 28 -- Message-ID: {001c01c5f012$4bc5a9d0$7b865290-at-keithhigrade} 9, 28 -- From: "Keith J Morris" {keith.morris-at-ucl.ac.uk} 9, 28 -- To: {Microscopy-at-microscopy.com} 9, 28 -- References: {200511211641.jALGfTHv030837-at-ns.microscopy.com} 9, 28 -- Subject: Re: [Microscopy] Re: TEM negative scanner 9, 28 -- Date: Wed, 23 Nov 2005 09:43:02 -0000 9, 28 -- MIME-Version: 1.0 9, 28 -- Content-Type: text/plain; 9, 28 -- format=flowed; 9, 28 -- charset="iso-8859-1"; 9, 28 -- reply-type=original 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-Priority: 3 9, 28 -- X-MSMail-Priority: Normal 9, 28 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2670 9, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2670 9, 28 -- X-UCL-MailScanner-Information: Please contact the UCL Helpdesk, helpdesk-at-ucl.ac.uk for more information 9, 28 -- X-UCL-MailScanner: Found to be clean 9, 28 -- X-UCL-MailScanner-SpamCheck: 9, 28 -- X-MailScanner-From: keith.morris-at-ucl.ac.uk ==============================End of - Headers==============================
By the way, If you are 'archiving' the TEM negatives in maximum resolution and possibly dumping the negative, I would buy the Nikon LS 900D - it's £2,500 but I'm sure the smaller Nikon scanners are all 35mm only. Compared to a digital camera on the TEM I suppose the price isn't bad. It can't scan photo's or paper though. From my experience of scanning slides it can be a daunting task working through an old archive at 3 minutes per photo just to scan the TEM negative, but for new output that may be fine. It would be great for my home slides though at 40s for 35mm.
Nikon details:
The SUPER COOLSCAN 9000 ED's multi-format capability is specifically designed for imaging professionals. Scanning is possible for 120/220, 35mm, 6 x 7, 6 x 9 positives, 16mm, electron microscope and other film formats. The 9000 ED's large-diameter Scanner Nikkor ED lens, 3-line CCD image sensor and LED light source with rod dispersion have all been improved for enhanced image quality with faster scanning speeds. These premium features give you the leading edge in professional desktop imaging.
1. Multiple film formats (120/220, 35mm, etc.) 2. 4,000 dpi true optical resolution 3. 16-bit A/D converter 4. Large-diameter new Scanner Nikkor ED lens 5. Improved rod dispersion LED illumination 6. High-speed scanning (35mm slide film: 40 seconds; 6 x 9: 185 seconds) 7. Newly-developed, high-quality 3-line CCD sensor 8. New advanced image processing algorithm for colour negative film 9. Multi-sample scanning 10. Quick AF & Quick Preview 11. High-speed IEEE 1394 interface 12. Scan Image Enhancer 13. Digital ICE4 Advanced (TM) Digital ICE Quad Advanced) with Digital ICE Professional(TM)
Regards Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
can I just remind you that Microtek does produce the i900 which is a large flatbed with full frame glassless holder much as the Agfa duoscan systems were made. It has a true optical scan resolution of 3200x6400dpi (as far as I know - must be careful here because I know there was a problem with the early Epsons), 4.2 dynamic range, scans up to 8x10 inch transpareny or legal format for paper scans, its 48bit colour USB 2.0 and firewire. It also comes with 'digital ice' and Silverfast software. Check out the spec on:
The only thing is that you would have to sort out your own e.m film holder if it's not one of the following: SnapTrans holders 35 mm slide holder; 35 mm filmstrip holder; 4"x5" film holder; 6 x 9 cm film holder. I think it would be the same problem for any/most of the other scanners. Ironically the do provide a glass holder as well.
The current UK prices seem to be between about 700 uk pounds which isn't bad for an 8x10inch glassless scanner - so you could be talking 'gift-horse' and oral observation.
I don't actually have one but I have used the older Microtek scanmaker 8700 for a couple of years now without problem.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Sunderland SR1 3SD UK
e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: keith.morris-at-ucl.ac.uk
Pat,
Not an easy task to accomplish. Back in the sixty's, the way to ID motor end plates was to inject methylene blue into the muscle near the nerve while still in the patient. A book "The Innervation of Muscle: A Biopsy Study" by C. Coers and A.L. Woolf, published by Charles C. Thomas explains in detail how this procedure is done.
If specimen is in blocks already, you might try doing immunohistochemistry on the semi-thin sections using something like S-100 (or another nerve antibody). Another idea may be to try enzyme histochemistry - with a PAP secondary step - on the fixed tissue before embedding, using something like cholinesterase.
I have never tried these, just some random thoughts.
Best of luck,
Ed
Edward P. Calomeni Director EM Lab The Ohio State University M018 Starling Loving Hall 320 W. 10th Ave. Columbus, OH 43210 614-293-5580 (office) 614-293-8806 (lab) edward.calomeni-at-osumc.edu -----Original Message----- X-from: pekysar-at-ucdavis.edu [mailto:pekysar-at-ucdavis.edu] Sent: Tuesday, November 22, 2005 9:38 PM To: Edward Calomeni
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Email: pekysar-at-ucdavis.edu Name: Pat Kysar
Organization: University of California, Davis, School of Medicine,Pathology
Title-Subject: [Filtered] Identifying motor endplates in thick sections
Question: Hi all, We have a pathologist who is conducting a study which necessitates the need to locate motor endplates in resin embedded muscle tissue. He would like to sucessfully locate them in thick sections so we can accurately cut thin sections which includes the area of interest. Does anyone have a protocol for staining the endplates on the thick sections (epoxy resin embedded) to facilitate a more accurate identification of these structures? The pathologist has tried finding the areas on Methylene blue/Azure B stained sections with a very low rate of success and desires a more accurate method. Any help would be appreciated. Thanks,
Pat Kysar EM Lab University of California, Davis School of Medicine, Pathology 1 Shields Davis, CA 95616 pekysar-at-ucdavis.edu
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Email: aolins-at-bowdoin.edu Name: Ada L. Olins
Organization: Bowdoin College
Title-Subject: [Filtered] digitize a negative
Question:
What are the most critical criteria to consider in order to optimize the quality of a digitized em negative? Please recommend a scanner or features of a scanner.
I've searched the Listserver archives and not found an answer to my question.
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Organization: Weill Medical College of Cornell University
Title-Subject: [Filtered] centrifuge tubes?
Question: Hi Everyone,
Don't know if such a thing exists (I didn't find anything via a quick search of a few vendors and google), but I'm trying to find centrifuge tubes that auto-open or vent during a spin. Any ideas?
Best regards, Nathan
-- Nathan O'Connor Blanchard Lab Department of Physiology and Biophysics Weill Medical College of Cornell University 1300 York Avenue New York, NY 10021
If you all remember, I posted a similar message asking suggestions on film scanners not long ago. I then did an extensive search myself, and here is the result.
First of all, I did not find too many professional grade film scanners around that will do large format of standard TEM films. Imacon models were the only ones I found that allows TEM film format, but they cost over $12K. The first scanner I tried was Nikon SuperCool Scan 900. It has an optical resolution of 4000 dpi, and Dmax of 4.8. It is perhaps one of the best film scanners for its price (around $2000), but unfortunately the largest film format it will do is 6 X 8 cm. I looked into whether or not the film carrier could be modified, but came to a conclusion that if I really wanted to get this Nikon scanner, I would have to trim my films, which I do not want to. I then bought an Epson Perfection 4990 Photo. It is a flatbed about $450. The optical resolution of this scanner is 4800 dpi, and Dmax 4.0. I consulted with a few knowledgeable people, and they all think with specifications like this, it should do an adequate job. But I did not like the images I got from it at all. It lacks continuous gray tone, so images look contrast and harsh.
The scanner I eventually purchased (about $1000) was Microtek Artixscan 1800f. It is a dual media unit. The top glass is for reflective scanning only, but it has a media insert holder that allow two 4x5" negatives. I can mask off this holder to fit TEM negatives in. This is better than using a flat bed with transparency adapter since it eliminates Moire bands (Thank you, Gary Gaugler, for telling me about this). This scanner offers the highest Dmax than any other flatbed scanners I could find (Dmax 4.8). But the down side is its limited resolution (1800 dpi). I debated for a while and decided it will suit what we need. We usually view our images on a monitor, and only print in small sizes for publication. We are now using this scanner with our new Apple 20-inch flat panel cinema, and very happy with it. However, I think another Microtek scanner, ScanMaker i900 might be a good choice too if not a better one. It cost $600. Rez, 3200 dpi; Dmax, 4.2.
One thing I learned was that you cannot judge a scanner by its specifications alone. You really need to try it out to see the end result. Unfortunately the companies do not set up demos for scanners at above price range. I end up returned twice and I am sure our purchasing department was very happy with me. ;-)
Thank you, everyone, and to US readers, enjoy the holiday weekend.
Hong Emory EM
==============================Original Headers============================== 7, 17 -- From hyi-at-emory.edu Wed Nov 23 08:21:12 2005 7, 17 -- Received: from sccrmhc14.comcast.net ([63.240.77.84]) 7, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jANELBQS028264 7, 17 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Nov 2005 08:21:12 -0600 7, 17 -- Received: from [24.30.86.130] (c-24-30-86-130.hsd1.ga.comcast.net[24.30.86.130]) 7, 17 -- by comcast.net (sccrmhc14) with SMTP 7, 17 -- id {2005112314200901400lrti5e} ; Wed, 23 Nov 2005 14:20:14 +0000 7, 17 -- Mime-Version: 1.0 (Apple Message framework v622) 7, 17 -- Message-Id: {daa522686e42f8b4f1ebe6063fefae1c-at-emory.edu} 7, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 17 -- To: Microscopy-at-microscopy.com 7, 17 -- From: Hong Yi {hyi-at-emory.edu} 7, 17 -- Subject: [Microscopy] Re: TEM negative scanner 7, 17 -- Date: Wed, 23 Nov 2005 09:20:09 -0500 7, 17 -- X-Mailer: Apple Mail (2.622) 7, 17 -- Content-Transfer-Encoding: 8bit 7, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jANELBQS028264 ==============================End of - Headers==============================
Hi listers We have been discussing scanners. Thank you all for the input.
What are the current recommendations for printers? There are two outputs I am interested in (one printer would be good, two is OK) 1) color micrographs 2) TEM micrographs
What do people use? What are you happy with?
Thank you
David
==============================Original Headers============================== 6, 20 -- From Elliott-at-arizona.edu Wed Nov 23 11:10:21 2005 6, 20 -- Received: from smtpgate.email.arizona.edu (deagol.email.Arizona.EDU [128.196.133.142]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jANHAKpr012236 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 23 Nov 2005 11:10:21 -0600 6, 20 -- Received: from localhost (gimli.email.arizona.edu [10.0.0.223]) 6, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 44BFFBBB8C2 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 23 Nov 2005 10:10:20 -0700 (MST) 6, 20 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 6, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id A3B74BC2528 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 23 Nov 2005 10:10:19 -0700 (MST) 6, 20 -- Mime-Version: 1.0 (Apple Message framework v623) 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- Message-Id: {8dbae08b600811d0b7f52f203cfb5114-at-arizona.edu} 6, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 6, 20 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 6, 20 -- From: David Elliott {Elliott-at-arizona.edu} 6, 20 -- Subject: printers 6, 20 -- Date: Wed, 23 Nov 2005 10:10:19 -0700 6, 20 -- X-Mailer: Apple Mail (2.623) 6, 20 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Some time ago there was an interesting post describing a method that allow the cuticle of an insect to bind to the resin so that they did not separate during sectioning. I printed it out and filed it in a safe place. now I cannot find it and clearly someone has stolen it. Does anyone out there recall this post? The person treated the insect with some stuff during embedding. Willing to pay big for this information.
Greg
- Gregory W. Erdos, Ph.D,
Assistant Director, Biotechnology Program Scientific Director, EM Core Lab P.O. Box 118525 University of Florida Gainesville, FL 32611 gwe-at-ufl.edu Phone: 352-392-1295 Fax: 352-846-0251
==============================Original Headers============================== 6, 21 -- From gwe-at-ufl.edu Wed Nov 23 12:26:33 2005 6, 21 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jANIQX5H022140 6, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 23 Nov 2005 12:26:33 -0600 6, 21 -- Received: from [128.227.60.168] (empc14419.dhcp.clas.ufl.edu [128.227.60.168]) 6, 21 -- by smtp.ufl.edu (8.13.4/8.13.4/2.5.1) with ESMTP id jANIQVsP1859686 6, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 23 Nov 2005 13:26:31 -0500 6, 21 -- Message-ID: {4384B459.3070307-at-ufl.edu} 6, 21 -- Date: Wed, 23 Nov 2005 13:26:33 -0500 6, 21 -- From: Greg Erdos {gwe-at-ufl.edu} 6, 21 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 6, 21 -- X-Accept-Language: en-us, en 6, 21 -- MIME-Version: 1.0 6, 21 -- To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com} 6, 21 -- Subject: Insect EM 6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- X-Spam-Status: hits=-0.001, required=5, tests=BAYES_44 6, 21 -- X-UFL-Spam-Status: hits=-0.001, required=5, tests=BAYES_44 6, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 6, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
best wishes, Wolfgang Muss, Salzburg X-from ListServer's Archive --------------------------------------------------- X-from: Tindall, Randy D. : TindallR-at-missouri.edu
Some time ago there was an interesting post describing a method that allow the cuticle of an insect to bind to the resin so that they did not separate during sectioning. I printed it out and filed it in a safe place. now I cannot find it and clearly someone has stolen it. Does anyone out there recall this post? The person treated the insect with some stuff during embedding. Willing to pay big for this information.
Greg
- Gregory W. Erdos, Ph.D,
Assistant Director, Biotechnology Program Scientific Director, EM Core Lab P.O. Box 118525 University of Florida Gainesville, FL 32611 gwe-at-ufl.edu Phone: 352-392-1295 Fax: 352-846-0251
==============================Original Headers============================== 6, 21 -- From gwe-at-ufl.edu Wed Nov 23 12:26:33 2005 6, 21 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jANIQX5H022140 6, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 23 Nov 2005 12:26:33 -0600 6, 21 -- Received: from [128.227.60.168] (empc14419.dhcp.clas.ufl.edu [128.227.60.168]) 6, 21 -- by smtp.ufl.edu (8.13.4/8.13.4/2.5.1) with ESMTP id jANIQVsP1859686 6, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 23 Nov 2005 13:26:31 -0500 6, 21 -- Message-ID: {4384B459.3070307-at-ufl.edu} 6, 21 -- Date: Wed, 23 Nov 2005 13:26:33 -0500 6, 21 -- From: Greg Erdos {gwe-at-ufl.edu} 6, 21 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 6, 21 -- X-Accept-Language: en-us, en 6, 21 -- MIME-Version: 1.0 6, 21 -- To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com} 6, 21 -- Subject: Insect EM 6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- X-Spam-Status: hits=-0.001, required=5, tests=BAYES_44 6, 21 -- X-UFL-Spam-Status: hits=-0.001, required=5, tests=BAYES_44 6, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 6, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
==============================Original Headers============================== 30, 28 -- From W.Muss-at-salk.at Wed Nov 23 13:01:20 2005 30, 28 -- Received: from Hermes.salk.at (hermes.lks.at [193.170.167.9]) 30, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jANJ1J22031399 30, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 23 Nov 2005 13:01:20 -0600 30, 28 -- Received: from localhost (localhost [127.0.0.1]) 30, 28 -- by hermes.lks.at (Postfix) with ESMTP id EE9515A9046; 30, 28 -- Wed, 23 Nov 2005 20:01:17 +0100 (CET) 30, 28 -- Received: from hermes.lks.at ([127.0.0.1]) 30, 28 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024) 30, 28 -- with ESMTP id 37807-09; Wed, 23 Nov 2005 20:01:17 +0100 (CET) 30, 28 -- Received: from c1pa003 (unknown [192.168.42.3]) 30, 28 -- by hermes.lks.at (Postfix) with SMTP id 9AF335A9041; 30, 28 -- Wed, 23 Nov 2005 20:01:17 +0100 (CET) 30, 28 -- Received: by localhost with Microsoft MAPI; Wed, 23 Nov 2005 20:01:14 +0100 30, 28 -- Message-ID: {01C5F068.A872FBA0.W.Muss-at-salk.at} 30, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at} 30, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at} 30, 28 -- To: "'gwe-at-ufl.edu'" {gwe-at-ufl.edu} 30, 28 -- Cc: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-MSA.Microscopy.Com} 30, 28 -- Subject: AW: [Microscopy] Insect EM 30, 28 -- Date: Wed, 23 Nov 2005 20:01:13 +0100 30, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at} 30, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor 30, 28 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211 30, 28 -- MIME-Version: 1.0 30, 28 -- Content-Type: text/plain; charset="us-ascii" 30, 28 -- Content-Transfer-Encoding: 7bit 30, 28 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at ==============================End of - Headers==============================
I am attemping to fix the small marine invertebrate Trichoplax adhaerens for conventional TEM usage. My lab grows Trichoplax in large glass petri dishes where they multiply by asexual budding and binary fission. Prior to fixation, we transfer the animals to small glass wells with filtered 36ppt seawater, where they move and lie flat on the bottom of the glass wells after about 10-20 minutes.
So far, every fixative I've tried results in the animals displaying what I call a "contraction response." About 3-5 seconds after rapid immersion in a fixative, the large flat Trichoplax immediately contract in an all-or-nothing response. They pull themselves inward, develop frilled edges along their perimeter, and lift off the bottom of the glass well temporarily before lying back down. Often, the animals curl in on themselves and are fixed in that position. The degree of this contraction response is variable, depending on how dissimilar the fixative is to their normal seawater. For example, a very hypertonic seawater solution (non-fixative) results in a severe contraction response, as does seawater buffered in cacodylate. Most of the fixatives I've tried are 4% glutaraldehyde based. My most successful fixative is 4% glutaraldehyde in 36ppt seawater, no cacodylate, pH 8.3. Nevertheless, this fixative causes the Trichoplax to undergo a mild contraction response. Even introducing a fixative one drop at a time results in the response.
In short, Trichoplax seems to be very sensitive to any changes in external environment. The contraction is problematic for my TEM study, so I am hoping to find a fixative that the animals do not respond to. Do you have any suggestions regarding either the fixative solution or the fixative protocol?
Thank you all in advance for your help - it is greatly appreciated!
Chad Kritzberger chad.kritzberger-at-yale.edu 203-436-1538 Osborne Memorial Labs, Yale University
==============================Original Headers============================== 6, 33 -- From chad.kritzberger-at-yale.edu Wed Nov 23 13:11:29 2005 6, 33 -- Received: from pantheon-po09.its.yale.edu (pantheon-po09.its.yale.edu [130.132.50.55]) 6, 33 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jANJBTJf007836 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 23 Nov 2005 13:11:29 -0600 6, 33 -- Received: from horde06.its.yale.edu (horde06.its.yale.edu [130.132.50.59]) 6, 33 -- by pantheon-po09.its.yale.edu (8.12.11/8.12.11) with ESMTP id jANJBT90016646 6, 33 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 23 Nov 2005 14:11:29 -0500 6, 33 -- Received: from horde06.its.yale.edu (localhost.localdomain [127.0.0.1]) 6, 33 -- by horde06.its.yale.edu (8.13.1/8.13.1) with ESMTP id jANJBStm009958 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 23 Nov 2005 14:11:28 -0500 6, 33 -- Received: (from apache-at-localhost) 6, 33 -- by horde06.its.yale.edu (8.13.1/8.13.1/Submit) id jANJBS67009957 6, 33 -- for Microscopy-at-Microscopy.Com; Wed, 23 Nov 2005 14:11:28 -0500 6, 33 -- Received: from yale128036062163.student.yale.edu 6, 33 -- (yale128036062163.student.yale.edu [128.36.62.163]) by www.mail.yale.edu 6, 33 -- (Horde MIME library) with HTTP; Wed, 23 Nov 2005 14:11:28 -0500 6, 33 -- Message-ID: {20051123141128.karmskj6s0gg840s-at-www.mail.yale.edu} 6, 33 -- Date: Wed, 23 Nov 2005 14:11:28 -0500 6, 33 -- From: Chad Kritzberger {chad.kritzberger-at-yale.edu} 6, 33 -- To: Microscopy-at-Microscopy.Com 6, 33 -- Subject: TEM - Problems with Trichoplax Fixation 6, 33 -- MIME-Version: 1.0 6, 33 -- Content-Type: text/plain; 6, 33 -- charset=ISO-8859-1 6, 33 -- Content-Disposition: inline 6, 33 -- Content-Transfer-Encoding: 7bit 6, 33 -- User-Agent: Internet Messaging Program (IMP) H3 (4.0.3) 6, 33 -- X-Originating-IP: 130.132.50.65 6, 33 -- X-Forwarded-For: 128.36.62.163 6, 33 -- X-Remote-Browser: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; 6, 33 -- Q312461; SV1) 6, 33 -- X-YaleITSMailFilter: Version 1.2b (attachment(s) not renamed) ==============================End of - Headers==============================
I don't know of a motor end plate stain that can be used on a resin semi thin section. We would normally locate NMJ by doing a cytochemical localisation before the processing and embedding. However, in an 'unstained' muscle block I would look firstly for a nerve bundle, then for a single nerve fibre running close to the muscle bundle. If you can locate a single nerve fibre then there is a good chance a NMJ is not far away.
You do have to develop a bit ofd an eye for them in the semi-thin section. They are quite small in relation to the other structures and even at high magn, can be very difficult to see. We describe them to people looking for one along the edge of a muscle bundle as "look for a section of the bundle edge that looks a little mottled, perhaps moth eaten, compared to the rest of the edge. As I say, the single nerve fibre is a good clue.
Good luck
Allan
On 23/11/2005, at 3:30 PM, pekysar-at-ucdavis.edu wrote:
} } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } ----------------------------------------------------------------------- } ---- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both pekysar-at-ucdavis.edu as well as the MIcroscopy } Listserver } ----------------------------------------------------------------------- } ---- } } Email: pekysar-at-ucdavis.edu } Name: Pat Kysar } } Organization: University of California, Davis, School of } Medicine,Pathology } } Title-Subject: [Filtered] Identifying motor endplates in thick } sections } } Question: Hi all, } We have a pathologist who is conducting a study which necessitates the } need to locate motor endplates in resin embedded muscle tissue. He } would like to sucessfully locate them in thick sections so we can } accurately cut thin sections which includes the area of interest. } Does anyone have a protocol for staining the endplates on the thick } sections (epoxy resin embedded) to facilitate a more accurate } identification of these structures? The pathologist has tried finding } the areas on Methylene blue/Azure B stained sections with a very low } rate of success and desires a more accurate method. } Any help would be appreciated. } Thanks, } } Pat Kysar } EM Lab } University of California, Davis } School of Medicine, Pathology } 1 Shields } Davis, CA 95616 } pekysar-at-ucdavis.edu } } ----------------------------------------------------------------------- } ---- } } ==============================Original } Headers============================== } 6, 12 -- From zaluzec-at-microscopy.com Tue Nov 22 20:30:18 2005 } 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 6, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } jAN2UGwP026218 } 6, 12 -- for {microscopy-at-microscopy.com} ; Tue, 22 Nov 2005 20:30:18 } -0600 } 6, 12 -- Mime-Version: 1.0 } 6, 12 -- X-Sender: (Unverified) } 6, 12 -- Message-Id: {p06110405bfa984ad2ada-at-[206.69.208.22]} } 6, 12 -- Date: Tue, 22 Nov 2005 20:30:15 -0600 } 6, 12 -- To: microscopy-at-microscopy.com } 6, 12 -- From: pekysar-at-ucdavis.edu (by way of MicroscopyListserver) } 6, 12 -- Subject: viaWWW: Identifying motor endplates in thick sections } 6, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers============================== } } Allan Mitchell Otago Centre for Electron Microscopy Department of Anatomy and Structural Biology School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254
EM Centre: http://ocem.otago.ac.nz/ Confocal Centre: http://occm.otago.ac.nz/ Department: http://anatomy.otago.ac.nz/
==============================Original Headers============================== 11, 22 -- From allan.mitchell-at-stonebow.otago.ac.nz Wed Nov 23 13:27:22 2005 11, 22 -- Received: from mailhub1.otago.ac.nz (mailhub1.otago.ac.nz [139.80.64.218]) 11, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jANJRKNN016910 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 23 Nov 2005 13:27:21 -0600 11, 22 -- Received: from galadriel.otago.ac.nz (galadriel.otago.ac.nz [139.80.64.213]) 11, 22 -- by mailhub1.otago.ac.nz (8.12.11/8.12.11) with ESMTP id jANJRIA8019417; 11, 22 -- Thu, 24 Nov 2005 08:27:19 +1300 11, 22 -- Received: from [139.80.40.154] (ou040154.otago.ac.nz [139.80.40.154]) 11, 22 -- by galadriel.otago.ac.nz (8.12.8/8.12.8) with ESMTP id jANJRIxH025194; 11, 22 -- Thu, 24 Nov 2005 08:27:18 +1300 (NZDT) 11, 22 -- In-Reply-To: {200511230230.jAN2UT2R027060-at-ns.microscopy.com} 11, 22 -- References: {200511230230.jAN2UT2R027060-at-ns.microscopy.com} 11, 22 -- Mime-Version: 1.0 (Apple Message framework v623) 11, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 11, 22 -- Message-Id: {8d2cb10cc6a7015b4d58c25e8aa24030-at-stonebow.otago.ac.nz} 11, 22 -- Content-Transfer-Encoding: 7bit 11, 22 -- Cc: microscopy-at-microscopy.com 11, 22 -- From: Allan Mitchell {allan.mitchell-at-stonebow.otago.ac.nz} 11, 22 -- Subject: Re: [Microscopy] viaWWW: Identifying motor endplates in thick sections 11, 22 -- Date: Thu, 24 Nov 2005 08:27:21 +1300 11, 22 -- To: pekysar-at-ucdavis.edu 11, 22 -- X-Mailer: Apple Mail (2.623) ==============================End of - Headers==============================
Have you tried cooling the critters first? In the 'frig at 4 deg C. This relaxes or slows many marine inverts so that they don't contract. Also, MgCl2 is used as an anaesthetic. Try 0.37 M MgCl2.6H20, that should be isotonic to seawater (adjust as needed). This will take a few to maybe 15 minutes. Or, try a few crystals of MgSO4. Relaxation is slower than with MgCl2, and more may need to be added after 1/2 hour or so. There are also various funs chemicals to try, most of them narcotics. MS-222 (in the Sigma catalog) works for fish and small crustaceans, it may work on Trichoplax. Gentle warming, CO2 (as seltzer water or bubbled in), and ethanol added dropwise also work for some animals.
Phil
Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 Sic hoc legare scis nimium eruditionis habes.
-----Original Message----- X-from: chad.kritzberger-at-yale.edu [mailto:chad.kritzberger-at-yale.edu] Sent: Wed 05/11/23 14:15 To: Oshel, Philip Eugene
Hi all microscopists,
I need advice on making amorphous C visible in the SEM. Basically, I have an uncovered thin section of a meteorite. This meteorite contains many small 100 to 1000 nm amorphous C spheres. The C is composed of small condensed aromatic units that are cross-linked with short aliphatic chains. I would like to post stain the C so that I can readily map and find the particles using a backscattered detector on one of our FEG-SEMs.
I have no experience with sample staining - so of all the stains out there, which one would be best for my sample?
Thanks,
Laurence
------------------------------------------------------------------------ -------------------------- Dr. Laurence A.J. Garvie Faculty Research Associate Department of Geological Sciences Arizona State University Tempe AZ 85287-1404 USA
I noticed that JEOL are selling a product called the Quantomix capsule which is a specimen holder which allows for the imaging of "wet" samples in an SEM. I'd be interested in any reports on these as it is something we are considering.
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==============================Original Headers============================== 15, 29 -- From Colin.Veitch-at-csiro.au Wed Nov 23 19:26:22 2005 15, 29 -- Received: from act-ironport-ext-out3.csiro.au (act-ironport-ext-out3.csiro.au [150.229.7.39]) 15, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAO1QLeh015289 15, 29 -- for {microscopy-at-msa.microscopy.com} ; Wed, 23 Nov 2005 19:26:22 -0600 15, 29 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 15, 29 -- by act-ironport-ext-out3.csiro.au with ESMTP; 24 Nov 2005 12:26:20 +1100 15, 29 -- X-IronPort-AV: i="3.97,367,1125842400"; 15, 29 -- d="scan'208"; a="70418649:sNHT32562768" 15, 29 -- Received: from exvicn1-mel.nexus.csiro.au ([138.194.3.60]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 15, 29 -- Thu, 24 Nov 2005 12:26:19 +1100 15, 29 -- Received: from exvic5-gex.nexus.csiro.au ([138.194.194.6]) by exvicn1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 15, 29 -- Thu, 24 Nov 2005 12:26:19 +1100 15, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 15, 29 -- content-class: urn:content-classes:message 15, 29 -- MIME-Version: 1.0 15, 29 -- Content-Type: text/plain; 15, 29 -- charset="us-ascii" 15, 29 -- Subject: Quantomix sample holders 15, 29 -- Date: Thu, 24 Nov 2005 12:26:19 +1100 15, 29 -- Message-ID: {32CDDDAA7161394599F0025494915749051D8F-at-exvic5-gex.nexus.csiro.au} 15, 29 -- X-MS-Has-Attach: 15, 29 -- X-MS-TNEF-Correlator: 15, 29 -- Thread-Topic: Quantomix sample holders 15, 29 -- Thread-Index: AcXwlhIF+mASH/QXSjWlfhPKG6VZjg== 15, 29 -- From: {Colin.Veitch-at-csiro.au} 15, 29 -- To: {microscopy-at-msa.microscopy.com} 15, 29 -- X-OriginalArrivalTime: 24 Nov 2005 01:26:19.0646 (UTC) FILETIME=[128601E0:01C5F096] 15, 29 -- Content-Transfer-Encoding: 8bit 15, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAO1QLeh015289 ==============================End of - Headers==============================
I just replaced an older Epson 820, which did a nice job of TEMs, with nice neutral blacks when set on "black only", but with a somewhat thin tonal range. HP seems to offer an advantage over Epson (the older ones, at least), as you get a new print head each time you replace the cartridge. The old Epson 820 (which has a print head build into the printer, not in the cartridge), seemed very finicky to me, and needed constant print head cleaning after a while. However, the older HPs made grays by using color inks in addition to black, and always seemed to leave a blue or purple tinge on the grayscale images.
The replacements are two similar, new HP printers, and just today I ran some test prints of transmission electron micrographs. One 8-1/2x11 printer, the HP 8450, and a larger format printer, the HP 8750; the 8450 is moderately priced, but the 8750 is a bit pricey and seems to offer no advantage besides the larger format for display prints. Both take three cartridges (a typical tri-color cartridge, a photo cartridge [stay away from the "photo blue" which is intended for intense blue skies and gives TEMs a blue tinge], and a photo gray). It's the photo gray that gives grayscale images a better tonal range, as it contains black (or dark gray, I can't be sure which), middle gray, and light gray inks. When set to "grayscale", with "black cartridge only" (the photo gray cartridge), the HPs do a very nice job of it, both on Kodak Bright White paper for drafts, and on high-gloss paper for final prints, and appear to give very neutral grays. So far, so good (but it's only the first day).
--Jan Factor
---------------------------------------11/23/05 Jan Robert Factor, Ph.D. Professor of Biology --------------------------------------- Natural Sciences Purchase College State University of New York 735 Anderson Hill Rd. Purchase, NY 10577 USA --------------------------------------- Office Tel: 914-251-6659 Office Fax: 914-251-6635 E-mail: jfactor-at-ns.purchase.edu or- jan.factor-at-purchase.edu ---------------------------------------
Elliott-at-arizona.edu wrote:11/23/05 } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi listers } We have been discussing scanners. Thank you all for the input. } } What are the current recommendations for printers? There are two } outputs I am interested in (one printer would be good, two is OK) } 1) color micrographs } 2) TEM micrographs } } What do people use? What are you happy with? } } Thank you } } David } } } ==============================Original Headers============================== } 6, 20 -- From Elliott-at-arizona.edu Wed Nov 23 11:10:21 2005 } 6, 20 -- Received: from smtpgate.email.arizona.edu (deagol.email.Arizona.EDU [128.196.133.142]) } 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jANHAKpr012236 } 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 23 Nov 2005 11:10:21 -0600 } 6, 20 -- Received: from localhost (gimli.email.arizona.edu [10.0.0.223]) } 6, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 44BFFBBB8C2 } 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 23 Nov 2005 10:10:20 -0700 (MST) } 6, 20 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) } 6, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id A3B74BC2528 } 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 23 Nov 2005 10:10:19 -0700 (MST) } 6, 20 -- Mime-Version: 1.0 (Apple Message framework v623) } 6, 20 -- Content-Transfer-Encoding: 7bit } 6, 20 -- Message-Id: {8dbae08b600811d0b7f52f203cfb5114-at-arizona.edu} } 6, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed } 6, 20 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} } 6, 20 -- From: David Elliott {Elliott-at-arizona.edu} } 6, 20 -- Subject: printers } 6, 20 -- Date: Wed, 23 Nov 2005 10:10:19 -0700 } 6, 20 -- X-Mailer: Apple Mail (2.623) } 6, 20 -- X-Virus-Scanned: amavisd-new at email.arizona.edu } ==============================End of - Headers============================== } }
==============================Original Headers============================== 7, 22 -- From jfactor-at-ns.purchase.edu Wed Nov 23 21:22:12 2005 7, 22 -- Received: from mta6.srv.hcvlny.cv.net (mta6.srv.hcvlny.cv.net [167.206.4.201]) 7, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAO3MCrr025128 7, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 23 Nov 2005 21:22:12 -0600 7, 22 -- Received: from [127.0.0.1] (ool-4357e60e.dyn.optonline.net [67.87.230.14]) 7, 22 -- by mta6.srv.hcvlny.cv.net 7, 22 -- (Sun Java System Messaging Server 6.2-4.03 (built Sep 22 2005)) 7, 22 -- with ESMTP id {0IQF007ZDW0YQF1P-at-mta6.srv.hcvlny.cv.net} for 7, 22 -- microscopy-at-msa.microscopy.com; Wed, 23 Nov 2005 22:22:12 -0500 (EST) 7, 22 -- Date: Wed, 23 Nov 2005 22:22:08 -0500 7, 22 -- From: Jan Factor {jfactor-at-ns.purchase.edu} 7, 22 -- Subject: Re: [Microscopy] printers 7, 22 -- In-reply-to: {200511231711.jANHBjDF013876-at-ns.microscopy.com} 7, 22 -- To: Elliott-at-arizona.edu, Microscopy Listserver {microscopy-at-msa.microscopy.com} 7, 22 -- Message-id: {438531E0.5020203-at-ns.purchase.edu} 7, 22 -- MIME-version: 1.0 7, 22 -- Content-type: text/plain; charset=us-ascii; format=flowed 7, 22 -- Content-transfer-encoding: 7BIT 7, 22 -- X-Accept-Language: en-us, en 7, 22 -- References: {200511231711.jANHBjDF013876-at-ns.microscopy.com} 7, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 7, 22 -- Gecko/20040804 Netscape/7.2 (ax) ==============================End of - Headers==============================
No experience but.... I have read that osmium tetroxide can be used as a primary fixative for marine invertebrates It might work faster as well. Take care to use a sealed container (it is very volatile) if trying to observe outside of a fume cupboard.
Dave
-----Original Message----- X-from: chad.kritzberger-at-yale.edu [mailto:chad.kritzberger-at-yale.edu] Sent: 23 November 2005 19:16 To: David Patton
Hello.
I am attemping to fix the small marine invertebrate Trichoplax adhaerens for conventional TEM usage. My lab grows Trichoplax in large glass petri dishes where they multiply by asexual budding and binary fission. Prior to fixation, we transfer the animals to small glass wells with filtered 36ppt seawater, where they move and lie flat on the bottom of the glass wells after about 10-20 minutes.
So far, every fixative I've tried results in the animals displaying what I call a "contraction response." About 3-5 seconds after rapid immersion in a fixative, the large flat Trichoplax immediately contract in an all-or-nothing response. They pull themselves inward, develop frilled edges along their perimeter, and lift off the bottom of the glass well temporarily before lying back down. Often, the animals curl in on themselves and are fixed in that position. The degree of this contraction response is variable, depending on how dissimilar the fixative is to their normal seawater. For example, a very hypertonic seawater solution (non-fixative) results in a severe contraction response, as does seawater buffered in cacodylate. Most of the fixatives I've tried are 4% glutaraldehyde based. My most successful fixative is 4% glutaraldehyde in 36ppt seawater, no cacodylate, pH 8.3. Nevertheless, this fixative causes the Trichoplax to undergo a mild contraction response. Even introducing a fixative one drop at a time results in the response.
In short, Trichoplax seems to be very sensitive to any changes in external environment. The contraction is problematic for my TEM study, so I am hoping to find a fixative that the animals do not respond to. Do you have any suggestions regarding either the fixative solution or the fixative protocol?
Thank you all in advance for your help - it is greatly appreciated!
Chad Kritzberger chad.kritzberger-at-yale.edu 203-436-1538 Osborne Memorial Labs, Yale University
==============================Original Headers============================== 6, 33 -- From chad.kritzberger-at-yale.edu Wed Nov 23 13:11:29 2005 6, 33 -- Received: from pantheon-po09.its.yale.edu (pantheon-po09.its.yale.edu [130.132.50.55]) 6, 33 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jANJBTJf007836 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 23 Nov 2005 13:11:29 -0600 6, 33 -- Received: from horde06.its.yale.edu (horde06.its.yale.edu [130.132.50.59]) 6, 33 -- by pantheon-po09.its.yale.edu (8.12.11/8.12.11) with ESMTP id jANJBT90016646 6, 33 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 23 Nov 2005 14:11:29 -0500 6, 33 -- Received: from horde06.its.yale.edu (localhost.localdomain [127.0.0.1]) 6, 33 -- by horde06.its.yale.edu (8.13.1/8.13.1) with ESMTP id jANJBStm009958 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 23 Nov 2005 14:11:28 -0500 6, 33 -- Received: (from apache-at-localhost) 6, 33 -- by horde06.its.yale.edu (8.13.1/8.13.1/Submit) id jANJBS67009957 6, 33 -- for Microscopy-at-Microscopy.Com; Wed, 23 Nov 2005 14:11:28 -0500 6, 33 -- Received: from yale128036062163.student.yale.edu 6, 33 -- (yale128036062163.student.yale.edu [128.36.62.163]) by www.mail.yale.edu 6, 33 -- (Horde MIME library) with HTTP; Wed, 23 Nov 2005 14:11:28 -0500 6, 33 -- Message-ID: {20051123141128.karmskj6s0gg840s-at-www.mail.yale.edu} 6, 33 -- Date: Wed, 23 Nov 2005 14:11:28 -0500 6, 33 -- From: Chad Kritzberger {chad.kritzberger-at-yale.edu} 6, 33 -- To: Microscopy-at-Microscopy.Com 6, 33 -- Subject: TEM - Problems with Trichoplax Fixation 6, 33 -- MIME-Version: 1.0 6, 33 -- Content-Type: text/plain; 6, 33 -- charset=ISO-8859-1 6, 33 -- Content-Disposition: inline 6, 33 -- Content-Transfer-Encoding: 7bit 6, 33 -- User-Agent: Internet Messaging Program (IMP) H3 (4.0.3) 6, 33 -- X-Originating-IP: 130.132.50.65 6, 33 -- X-Forwarded-For: 128.36.62.163 6, 33 -- X-Remote-Browser: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; 6, 33 -- Q312461; SV1) 6, 33 -- X-YaleITSMailFilter: Version 1.2b (attachment(s) not renamed) ==============================End of - Headers==============================
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==============================Original Headers============================== 18, 33 -- From David.Patton-at-uwe.ac.uk Thu Nov 24 05:44:47 2005 18, 33 -- Received: from mailapp01.uwe.ac.uk (mailapp01.uwe.ac.uk [164.11.132.61]) 18, 33 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jAOBikjk007667 18, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 24 Nov 2005 05:44:47 -0600 18, 33 -- Received: from (164.11.132.62) by mailapp01.uwe.ac.uk via smtp 18, 33 -- id 1310_ac83f60c_5cdf_11da_954f_0002b3c946e4; 18, 33 -- Thu, 24 Nov 2005 11:44:37 +0000 18, 33 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 18, 33 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121]) 18, 33 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 18, 33 -- 2005)) with ESMTP id {0IQG000A4JAE0M-at-mta02.uwe.ac.uk} for 18, 33 -- Microscopy-at-Microscopy.Com; Thu, 24 Nov 2005 11:44:38 +0000 (GMT) 18, 33 -- Date: Thu, 24 Nov 2005 11:44:38 +0000 18, 33 -- From: David Patton {David.Patton-at-uwe.ac.uk} 18, 33 -- Subject: RE: [Microscopy] TEM - Problems with Trichoplax Fixation 18, 33 -- To: chad.kritzberger-at-yale.edu 18, 33 -- Cc: Microscopy-at-Microscopy.Com 18, 33 -- Message-id: {F247F674896BE243AD8263C5280E2BDBF84F6D-at-NBUEGEN01} 18, 33 -- MIME-version: 1.0 18, 33 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5.6944.0 18, 33 -- Content-type: text/plain; charset=us-ascii 18, 33 -- Content-class: urn:content-classes:message 18, 33 -- Thread-topic: [Microscopy] TEM - Problems with Trichoplax Fixation 18, 33 -- Thread-index: AcXwYk9OjgSozmq+RSW63IRLmQlcEgAic+GQ 18, 33 -- X-MS-Has-Attach: 18, 33 -- X-MS-TNEF-Correlator: 18, 33 -- X-NAI-Spam-Score: -0 18, 33 -- X-NAI-Spam-Rules: 1 Rules triggered 18, 33 -- BAYES_44=-0 18, 33 -- X-NAIMIME-Disclaimer: 1 18, 33 -- X-NAIMIME-Modified: 1 18, 33 -- Content-Transfer-Encoding: 8bit 18, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jAOBikjk007667 ==============================End of - Headers==============================
I've just been looking at some thin crystals of an organic compound, sitting on a carbon film, under bright-field TEM. When in exact focus, they appear much less constrasty than when the focus knob is turned to the left of the right. When turned to the right, dark fringes appear around the crystals. I would like to ask:
(1) Which of the two , left or right, is over/under focus?
(2) Why are they more constrasty overall when over or under-focussed?
(3) Why are they more contrasty at lower magnification?
(Things were much easier when looking at shadowed-carbon replicas of polymer surfaces!)
Any help would be much appreciated.
----------------------------------- Robert H. Olley Reply to: R.H.Olley-at-reading.ac.uk URL: http://www.rdg.ac.uk/~spsolley -----------------------------------
==============================Original Headers============================== 10, 21 -- From hinmeigeng-at-hotmail.com Thu Nov 24 06:32:50 2005 10, 21 -- Received: from hotmail.com (bay101-f6.bay101.hotmail.com [64.4.56.16]) 10, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAOCWoFP017008 10, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 24 Nov 2005 06:32:50 -0600 10, 21 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 10, 21 -- Thu, 24 Nov 2005 04:32:49 -0800 10, 21 -- Message-ID: {BAY101-F6240047D447DCDCC6C3EFCA540-at-phx.gbl} 10, 21 -- Received: from 64.4.56.200 by by101fd.bay101.hotmail.msn.com with HTTP; 10, 21 -- Thu, 24 Nov 2005 12:32:49 GMT 10, 21 -- X-Originating-IP: [134.225.1.161] 10, 21 -- X-Originating-Email: [hinmeigeng-at-hotmail.com] 10, 21 -- X-Sender: hinmeigeng-at-hotmail.com 10, 21 -- Reply-To: R.H.Olley-at-reading.ac.uk 10, 21 -- From: "Robert H. Olley" {hinmeigeng-at-hotmail.com} 10, 21 -- To: Microscopy-at-MSA.Microscopy.Com 10, 21 -- Cc: R.H.Olley-at-reading.ac.uk 10, 21 -- Subject: Contrast and focus in TEM 10, 21 -- Date: Thu, 24 Nov 2005 12:32:49 +0000 10, 21 -- Mime-Version: 1.0 10, 21 -- Content-Type: text/plain; format=flowed 10, 21 -- X-OriginalArrivalTime: 24 Nov 2005 12:32:49.0708 (UTC) FILETIME=[2E7536C0:01C5F0F3] ==============================End of - Headers==============================
The Max Planck Institute of Molecular Cell Biology and Genetics in Dresden, Germany is now advertising for good people who would be willing to help us in running core facilities in our international institute ( more than 150 scientists from more then 30 countries). Currently there are three positions open:
Facility Leader - Electron Microscopy Facility http://www.mpi-cbg.de/research/jobs/em.html
If you are interested in these positions, please do not hesitate to contact me.
If you know someone who might be also interested in one of the above mentioned positions, please forward my email to him/her. The deadline for the appliactions is 9. December 2005. Thank you very much for your kind assistance in advance.
Regards
Jan
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx Jan Peychl, M.D., Ph.D. Service Leader Light Microscopy Facility Max Planck Institute of Molecular Cell Biology and Genetics Pfotenhauerstrasse 108 01307 Dresden Germany
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==============================Original Headers============================== 15, 18 -- From jb_sanderson-at-yahoo.com Thu Nov 24 07:35:56 2005 15, 18 -- Received: from web42005.mail.yahoo.com (web42005.mail.yahoo.com [66.218.93.173]) 15, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jAODZtdl026415 15, 18 -- for {microscopy-at-msa.microscopy.com} ; Thu, 24 Nov 2005 07:35:56 -0600 15, 18 -- Received: (qmail 74682 invoked by uid 60001); 24 Nov 2005 13:35:55 -0000 15, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 15, 18 -- s=s1024; d=yahoo.com; 15, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 15, 18 -- b=05d+bJBcFyjPMAaOj+qx0AtzchE4n3yr8MiGQpxwmgk7b+mhksj2Y69aWgKHiuP6cpmp5huBBVS2MmiVTzwZQIuAhczyubQmYvmNUGN1jtE0KyI1C2dv6N3/t1QARFtnFmlYVmTmBMCXg9Yf0+Gy275f0G7zt3XrTIarB+nkqJQ= ; 15, 18 -- Message-ID: {20051124133555.74680.qmail-at-web42005.mail.yahoo.com} 15, 18 -- Received: from [141.5.11.5] by web42005.mail.yahoo.com via HTTP; Thu, 24 Nov 2005 13:35:55 GMT 15, 18 -- Date: Thu, 24 Nov 2005 13:35:55 +0000 (GMT) 15, 18 -- From: Jeremy Sanderson {jb_sanderson-at-yahoo.com} 15, 18 -- Subject: 3 open positions for miscoscopists at the Max Planck Institute (MPI-CBG), Dresden, Germany 15, 18 -- To: microscopy-at-msa.microscopy.com 15, 18 -- MIME-Version: 1.0 15, 18 -- Content-Type: text/plain; charset=iso-8859-1 15, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I'm sure you will get much more detailed answers when the USA wakes up, but here's my two pence worth.
When you turn to the right and see a dark fringe at the edge of objects then it is overfocussed and a light fringe is underfocussed when you turn to the left. These are Fresnel fringes and are produced because of diffraction contrast which is a result of scattering of light/electrons at edges. It can and is quite often acceptable to defocus slightly to increase contrast but this should normally only be slightly underfocussed. The logic being that bright fringes will enhace the dark edges beside them, but dark fringes will introduce more apparent dark structures.
When you examine specimens at low magnification amplitude contrast makes a greater contribution to the image than phase contrast. Amplitude contrast is generated by the influence of the specimens atomic nuclei on electrons in the beam. The higher the mass of the nucleus the greater the 'scattering power' of that area of the specimen and the more electrons scattered out of the main beam path the darker that area. Amorphous samples simply produce more or less contrast by mass alone although crystals scatter electrons in much more defined ways due to the interaction of the 'wavelength' of the electrons, the lattice spacing in the crystal and its angle of tilt.
There are many good books on this subject, but here's the first one that came to hand: Principles and Practice of Electron Microscope Operation; A.W. Agar, R.H. Alderson and D. Chescoe; Pub North Holland (3rd print 1980) ISBN 0 72044255 9 where chapter 3 on image formation is particularly appropriate.
My apologies to crystallographers everywhere for a simple biologists explanation of diffraction contrast without once mentioning Bragg.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Sunderland SR1 3SD UK tel no: +44 (0)191 515 2872 e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: hinmeigeng-at-hotmail.com
Your description is right on target. When I was learning to use the em (in the dark ages, with an RCA-EMU3), I was taught to obtain best focus by experimenting first with a holey sample so that I could adjust what my eyes thought was best focus (because we are uniquely sensitive to contrast) to the true focus. I then would focus on a sample to what I thought was best, and then adjust the focus setting by a few notches. The issue of whether you want to include the underfocus fringes in your image becomes, then, an issue of whether you want to include the optical artifact for contrast, or you are interested in the "true" edge of your objects.
Date sent: Thu, 24 Nov 2005 08:14:13 -0600 To: jbs-at-temple.edu X-from: malcolm.haswell-at-sunderland.ac.uk Send reply to: malcolm.haswell-at-sunderland.ac.uk
Hello,
I might be wrong, but seem to have a little different understanding from the posts so far to these concepts of very importance in both theory and practice so that I reply, hoping to solicit more insights. In short,
1) Slight under-focus (OL knob counterclockwise adjustment from the least contrast or true focus point) not only makes image look good, but provides better resolution.
2) There are only two types of contrast mechanisms in my opinion: amplitude contrast and phase contrast. Amplitude contrast includes a) diffraction contrast, b) mass-thickness contrast, and c) Z-contrast (a special case of mass-thickness contrast).
Also due to the locality nature, Z-contrast and phase contrast can normally be thought responsible for or capable of providing lattice resolution (you may argue with in the cases of imaging with several diffraction spots) or atomic resolution.
Happy holiday or vacation (to those who don't view Thanksgiving as a holiday)!
Chaoying Ni W.M. Keck Electron Microscopy Facility Facility location: 022 Spencer Laboratory Mailing address: 201 duPont Hall Dept. of Materials Sci. and Eng. University of Delaware Newark, DE 19716 (302) 831-2318 (Phone) (302) 831-4545 (Fax) http://eml.masc.udel.edu
-----Original Message----- X-from: malcolm.haswell-at-sunderland.ac.uk [mailto:malcolm.haswell-at-sunderland.ac.uk] Sent: Thursday, November 24, 2005 9:16 AM To: cni-at-UDel.Edu
Robert
I'm sure you will get much more detailed answers when the USA wakes up, but here's my two pence worth.
When you turn to the right and see a dark fringe at the edge of objects then it is overfocussed and a light fringe is underfocussed when you turn to the left. These are Fresnel fringes and are produced because of diffraction contrast which is a result of scattering of light/electrons at edges. It can and is quite often acceptable to defocus slightly to increase contrast but this should normally only be slightly underfocussed. The logic being that bright fringes will enhace the dark edges beside them, but dark fringes will introduce more apparent dark structures.
When you examine specimens at low magnification amplitude contrast makes a greater contribution to the image than phase contrast. Amplitude contrast is generated by the influence of the specimens atomic nuclei on electrons in the beam. The higher the mass of the nucleus the greater the 'scattering power' of that area of the specimen and the more electrons scattered out of the main beam path the darker that area. Amorphous samples simply produce more or less contrast by mass alone although crystals scatter electrons in much more defined ways due to the interaction of the 'wavelength' of the electrons, the lattice spacing in the crystal and its angle of tilt.
There are many good books on this subject, but here's the first one that came to hand: Principles and Practice of Electron Microscope Operation; A.W. Agar, R.H. Alderson and D. Chescoe; Pub North Holland (3rd print 1980) ISBN 0 72044255 9 where chapter 3 on image formation is particularly appropriate.
My apologies to crystallographers everywhere for a simple biologists explanation of diffraction contrast without once mentioning Bragg.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Sunderland SR1 3SD UK tel no: +44 (0)191 515 2872 e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: hinmeigeng-at-hotmail.com
Hi,
It seems about time to add a post from California (yes, some of us are finally awake on the left coast).
As far as I can see, both (all) of the contributed points of view are correct.
Underfocus by a few hundred Angstroms will indeed enhance contrast and improve resolution. The explanation was first given by Otto Scherzer in his famous "20/20" paper on page 20 of volume 20 of J. Appl. Phys. [Scherzer, O. (1949). "The theoretical resolution limit of the electron microscope" J. Appl. Phys. 20, 20-29].
Scherzer describes how an underfocus of minus sqrt (Cs * wavelength) optimizes transfer of spatial frequencies into the image from the electron wave leaving the specimen -- by balancing (positive) phase shifts due to spherical aberration with (negative) shifts from underfocus to form a kind of quarter-wave plate. Optimum underfocus ranges from -750 Angstrom for a spherical aberration of 1 mm at 100 kV to half that for a Cs of 0.5 mm at 300 kV.
Be aware that too much underfocus (or overfocus) will add too much negative (or positive) phase shift and "scramble" the image so it no longer shows a simple projection of the specimen.
There is more on high resolution and focus in many publications -- the one I like is the paper "Resolution in high-resolution electron microscopy", M.A. O'Keefe, Ultramicroscopy 47 (1992) 282-297. ;-)
For TEM of organic molecules see papers by John Fryer -- especially J R Fryer 1993 J. Phys. D: Appl. Phys. 26 B137-B144. There's also M.A. O'Keefe, J.R. Fryer and D.J. Smith, Acta Cryst. A39 (1983) 838-847.
Happy Thanksgiving, Mike
Michael A. O'Keefe National Center for Electron Microscopy Lawrence Berkeley National Laboratory Berkeley, CA 94720
----- Original Message ----- X-from: cni-at-udel.edu
p.s. If anyone would like a calculator program (PC only) that will compute several TEM parameters, such as optimum defocus, let me know and I'll email it to you. It uses reverse Polish -- to compute optimum defocus for a voltage of 300kV and Cs of 1.2 mm, you would enter "1.2 300 wavl opt". Mike ----- Original Message ----- X-from: MAOKeefe-at-lbl.gov
Hi,
You might want to have a look at a few useful webpages:
a focusing simulator: http://www.umsl.edu/~fraundor/epc/index.html
contrast transfer function: http://clik.to/ctfexplorer http://ncmi.bcm.tmc.edu/homs/wen/ctf/ctfapplet.html
As we grow older we acquire wisdom, but can't remember where we put it.- DA
Philip Koeck Karolinska Inst. and University College of S. Stockholm Dept. of Bioscience at Novum tel: +46 8 608 9186 fax: +46 8 608 9290 web: http://www.csb.ki.se/users/philip/philown.html
-----Original Message----- X-from: hinmeigeng-at-hotmail.com [mailto:hinmeigeng-at-hotmail.com] Sent: 24 November 2005 13:37 To: Philip.Koeck-at-biosci.ki.se
Hi All!
I've just been looking at some thin crystals of an organic compound, sitting
on a carbon film, under bright-field TEM. When in exact focus, they appear much less constrasty than when the focus knob is turned to the left of the right. When turned to the right, dark fringes appear around the crystals. I would like to ask:
(1) Which of the two , left or right, is over/under focus?
(2) Why are they more constrasty overall when over or under-focussed?
(3) Why are they more contrasty at lower magnification?
(Things were much easier when looking at shadowed-carbon replicas of polymer
surfaces!)
Any help would be much appreciated.
----------------------------------- Robert H. Olley Reply to: R.H.Olley-at-reading.ac.uk URL: http://www.rdg.ac.uk/~spsolley -----------------------------------
==============================Original Headers============================== 10, 21 -- From hinmeigeng-at-hotmail.com Thu Nov 24 06:32:50 2005 10, 21 -- Received: from hotmail.com (bay101-f6.bay101.hotmail.com [64.4.56.16]) 10, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAOCWoFP017008 10, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 24 Nov 2005 06:32:50 -0600 10, 21 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 10, 21 -- Thu, 24 Nov 2005 04:32:49 -0800 10, 21 -- Message-ID: {BAY101-F6240047D447DCDCC6C3EFCA540-at-phx.gbl} 10, 21 -- Received: from 64.4.56.200 by by101fd.bay101.hotmail.msn.com with HTTP; 10, 21 -- Thu, 24 Nov 2005 12:32:49 GMT 10, 21 -- X-Originating-IP: [134.225.1.161] 10, 21 -- X-Originating-Email: [hinmeigeng-at-hotmail.com] 10, 21 -- X-Sender: hinmeigeng-at-hotmail.com 10, 21 -- Reply-To: R.H.Olley-at-reading.ac.uk 10, 21 -- From: "Robert H. Olley" {hinmeigeng-at-hotmail.com} 10, 21 -- To: Microscopy-at-MSA.Microscopy.Com 10, 21 -- Cc: R.H.Olley-at-reading.ac.uk 10, 21 -- Subject: Contrast and focus in TEM 10, 21 -- Date: Thu, 24 Nov 2005 12:32:49 +0000 10, 21 -- Mime-Version: 1.0 10, 21 -- Content-Type: text/plain; format=flowed 10, 21 -- X-OriginalArrivalTime: 24 Nov 2005 12:32:49.0708 (UTC) FILETIME=[2E7536C0:01C5F0F3] ==============================End of - Headers==============================
==============================Original Headers============================== 27, 25 -- From Philip.Koeck-at-biosci.ki.se Fri Nov 25 02:27:07 2005 27, 25 -- Received: from smtp.biosci.ki.se (smtp.biosci.ki.se [130.237.109.196]) 27, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAP8R560027793 27, 25 -- for {microscopy-at-microscopy.com} ; Fri, 25 Nov 2005 02:27:07 -0600 27, 25 -- Received: from smtp.biosci.ki.se (localhost [127.0.0.1]) 27, 25 -- by localhost (Postfix) with ESMTP id 3AF2A4FF20; 27, 25 -- Fri, 25 Nov 2005 09:27:04 +0100 (CET) 27, 25 -- Received: from whale.csb.ki.se (whale.csb.ki.se [130.237.109.8]) 27, 25 -- by smtp.biosci.ki.se (Postfix) with SMTP id 2CAD24FEBC; 27, 25 -- Fri, 25 Nov 2005 09:27:04 +0100 (CET) 27, 25 -- Received: from rapana.csb.ki.se by whale.csb.ki.se (5.65v4.0/1.1.10.5/03Feb98-0237PM) 27, 25 -- id AA04454; Fri, 25 Nov 2005 09:27:03 +0100 27, 25 -- Message-Id: {10511250827.AA04454-at-whale.csb.ki.se} 27, 25 -- From: "Philip Koeck" {Philip.Koeck-at-biosci.ki.se} 27, 25 -- To: {hinmeigeng-at-hotmail.com} , {microscopy-at-microscopy.com} 27, 25 -- Subject: RE: [Microscopy] Contrast and focus in TEM 27, 25 -- Date: Fri, 25 Nov 2005 09:29:05 +0100 27, 25 -- Mime-Version: 1.0 27, 25 -- Content-Type: text/plain; 27, 25 -- charset="us-ascii" 27, 25 -- Content-Transfer-Encoding: 7bit 27, 25 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353 27, 25 -- X-Mimeole: Produced By Microsoft MimeOLE V6.00.2900.2180 27, 25 -- In-Reply-To: {200511241236.jAOCat0w022273-at-ns.microscopy.com} 27, 25 -- Thread-Index: AcXw88HatYz0ZHAOSvKvcyxk6n9xEAApVbbQ ==============================End of - Headers==============================
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Email: neapit-at-yahoo.com Name: Nea Pitulis
Organization: Medical school University of Athens-Greece
Title-Subject: [Filtered] Molecular biology
Question: Hi everybody. I'm a PhD student in the med school with a degree in chemistry. I want to know where I can found info about microscopy techniques in the field of molecular biology.
I'm looking for a reference sample for calibration of camera lenght for diffraction and magnification (mainly for High Resolution). I already tried several sample (sold by Agar scientific):
"Oriented single crystal gold foil" for camera length and high resolution "Evaporated aluminium film" for camera length "Evaporated Thallous chloride" for camera length and High Resolution "Graphitised Carbon Black" for camera length and High Resolution.
However I obtain some non negligible shift on dspacings between various calibration standards (around 1% or 2%) these shifts are reproducible and thus are not due to alignement problem or bad eucentric position.
As this sample are not sold with a control certifical does someone know their real accuracy? Or is their an explanation to this errors?
A more expensive calibration standard "MAG*I*CAL" is sold, does someone has experienced it?
Any help would be much appreciated.
Patrick Weisbecker LCTS / PESSAC FRANCE
___________________________________________________________________________ Appel audio GRATUIT partout dans le monde avec le nouveau Yahoo! Messenger Téléchargez cette version sur http://fr.messenger.yahoo.com
==============================Original Headers============================== 6, 18 -- From weis183-at-yahoo.fr Fri Nov 25 12:46:21 2005 6, 18 -- Received: from web26701.mail.ukl.yahoo.com (web26701.mail.ukl.yahoo.com [217.146.176.64]) 6, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jAPIkKVl029743 6, 18 -- for {microscopy-at-microscopy.com} ; Fri, 25 Nov 2005 12:46:21 -0600 6, 18 -- Received: (qmail 83462 invoked by uid 60001); 25 Nov 2005 18:46:20 -0000 6, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 18 -- s=s1024; d=yahoo.fr; 6, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 6, 18 -- b=zqvqj4MJ1ah3TmvbRRW6dLcUWGOX+CWo7IQE9wmSHS+b1aaLeYrLIr2b/PJ8RLPLX18MBGXefhPBC+zooLTxw82HpvxLeuq44++Ta8W0pm1S8OaC4sZaj0XYuxdUCH438ucPexJNpxCarQ+VC7Z/ACpD0EEX5S1Fbz2ChVT6Toc= ; 6, 18 -- Message-ID: {20051125184620.83460.qmail-at-web26701.mail.ukl.yahoo.com} 6, 18 -- Received: from [84.5.133.20] by web26701.mail.ukl.yahoo.com via HTTP; Fri, 25 Nov 2005 19:46:20 CET 6, 18 -- Date: Fri, 25 Nov 2005 19:46:20 +0100 (CET) 6, 18 -- From: Patrick Weisbecker {weis183-at-yahoo.fr} 6, 18 -- Subject: TEM calibration standard 6, 18 -- To: microscopy-at-microscopy.com 6, 18 -- MIME-Version: 1.0 6, 18 -- Content-Type: text/plain; charset=iso-8859-1 6, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I would like to start off with two questions. Is there any way that you might have prepared this sample without embedding and preparing it as a thin section? How are you sure that such C spheres are present?
I expect that the C spheres would virtually disappear in the epoxy embedding medium which prompts your question about staining. If no epoxy was present, I suspect the spheres would show up nicely against the light background of the meteorite.
Since you cannot very well be using BSE to image the sample, how do you know the spheres are present and how do you know their chemical nature or size? If someone else has suggested or claimed their presence, how did they perform the characterization?
I would be interested in hearing the outcome of this exercise. It sounds like a challenge to do this by BSE even in a FEG-SEM, but I could be mistaken.
I have a Robinson Detector that is problematic and in need of repair. Strangely enough, a search of the web comes up blank on the manufacturer or places that fix them.
Does anyone have contact info?
Great list by the way! As the rare hobbyist with an SEM, this is my only contact with the EM world and it's much appreciated.
Tom Kaye
==============================Original Headers============================== 8, 20 -- From tom-at-tomkaye.com Sat Nov 26 22:45:32 2005 8, 20 -- Received: from tomkaye.com (mxgateway.techpro.com [64.4.207.8]) 8, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAR4jWLu013484 8, 20 -- for {Microscopy-at-microscopy.com} ; Sat, 26 Nov 2005 22:45:32 -0600 8, 20 -- Received: from tkdell [24.13.155.164] by tomkaye.com with ESMTP 8, 20 -- (SMTPD32-8.14) id A9E9AF3B0108; Sat, 26 Nov 2005 22:45:29 -0600 8, 20 -- From: "Tom" {tom-at-tomkaye.com} 8, 20 -- To: {Microscopy-at-microscopy.com} 8, 20 -- Subject: Robinson Detector Repair?? 8, 20 -- Date: Sat, 26 Nov 2005 22:43:28 -0600 8, 20 -- Message-ID: {035701c5f30d$1d15cea0$030a1aac-at-tkdell} 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Type: text/plain; 8, 20 -- charset="iso-8859-1" 8, 20 -- Content-Transfer-Encoding: 7bit 8, 20 -- X-Priority: 3 (Normal) 8, 20 -- X-MSMail-Priority: Normal 8, 20 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2910.0) 8, 20 -- Importance: Normal 8, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
Tom Kaye wrote: ====================================================== Hello All,
I have a Robinson Detector that is problematic and in need of repair. Strangely enough, a search of the web comes up blank on the manufacturer or places that fix them.
Does anyone have contact info?
Great list by the way! As the rare hobbyist with an SEM, this is my only contact with the EM world and it's much appreciated. ===================================================== The website you are looking for is http://www.etpsemra.com.au/
Dr. Vivian Robinson runs the company "hands on" and if you contact them through their website in Australia, you could very well hear from Dr. Robinson himself.
SPI Supplies has been providing sales and service for their detectors, see URL http://www.2spi.com/catalog/instruments/robinson-backscattered-electron-BSE-detector.shtml
Alternatively, you can contact me off-line and I will try my best to give you the assistance you require.
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.2spi.com ######################## ============================================
==============================Original Headers============================== 16, 25 -- From cgarber-at-2spi.com Sun Nov 27 08:35:39 2005 16, 25 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 16, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAREZd0h003300 16, 25 -- for {microscopy-at-microscopy.com} ; Sun, 27 Nov 2005 08:35:39 -0600 16, 25 -- Received: from ibm1x23g2abfyg ([70.5.216.231]) 16, 25 -- by diskless5.axs2000.net (8.12.11/8.12.11) with SMTP id jAREZPWf031563; 16, 25 -- Sun, 27 Nov 2005 09:35:33 -0500 16, 25 -- X-IDV-FirstRcvd: [70.5.216.231] 16, 25 -- X-IDV-HELO: ibm1x23g2abfyg 16, 25 -- Message-ID: {004301c5f35f$ceab4260$e7d80546-at-ibm1x23g2abfyg} 16, 25 -- Reply-To: "Garber, Charles A." {cgarber-at-2spi.com} 16, 25 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 16, 25 -- To: {microscopy-at-microscopy.com} 16, 25 -- Subject: Robinson BSE detectors website 16, 25 -- Date: Sun, 27 Nov 2005 09:24:57 -0500 16, 25 -- MIME-Version: 1.0 16, 25 -- Content-Type: text/plain; 16, 25 -- format=flowed; 16, 25 -- charset="Windows-1252"; 16, 25 -- reply-type=original 16, 25 -- Content-Transfer-Encoding: 7bit 16, 25 -- X-Priority: 3 16, 25 -- X-MSMail-Priority: Normal 16, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 16, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
Colin Veitch wrote: ======================================================== I noticed that JEOL are selling a product called the Quantomix capsule which is a specimen holder which allows for the imaging of "wet" samples in an SEM. I'd be interested in any reports on these as it is something we are considering. ======================================================== The Quantomix® capsules certainly do "work". I have seen them demonstrated on several different occasions at trade shows and on the SEMs of several different manufacturers. The technique is very exciting.
However I mention there is an alternative way to make an environmental ( vacuum compatible) cell, and that is with the use of silicon nitride membrane window grids, two glued together, see URL http://www.2spi.com/catalog/instruments/silicon-nitride.shtml
There is an example of the use of the SPI Supplies® Brand silicon nitride membrane window grids toward the bottom of the page for an "environmental cell" application.
Disclaimer: SPI Supplies offers a competing system to the Quantomix capsules so we would have a vested interest in promoting the use of our SPI Supplies® Brand silicon nitride membrane window grid system instead of the polymeric window Quantomix capsules.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.2spi.com ######################## ============================================
==============================Original Headers============================== 14, 25 -- From cgarber-at-2spi.com Sun Nov 27 12:11:40 2005 14, 25 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 14, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jARIBeTx014197 14, 25 -- for {microscopy-at-msa.microscopy.com} ; Sun, 27 Nov 2005 12:11:40 -0600 14, 25 -- Received: from ibm1x23g2abfyg ([70.5.216.231]) 14, 25 -- by diskless5.axs2000.net (8.12.11/8.12.11) with SMTP id jARIBX2x009937 14, 25 -- for {microscopy-at-msa.microscopy.com} ; Sun, 27 Nov 2005 13:11:38 -0500 14, 25 -- X-IDV-FirstRcvd: [70.5.216.231] 14, 25 -- X-IDV-HELO: ibm1x23g2abfyg 14, 25 -- Message-ID: {011701c5f37d$ffcaa7a0$e7d80546-at-ibm1x23g2abfyg} 14, 25 -- Reply-To: "Garber, Charles A." {cgarber-at-2spi.com} 14, 25 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 14, 25 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 14, 25 -- Subject: =?Windows-1252?Q?Quantomix=AE_sample_holders?= 14, 25 -- Date: Sun, 27 Nov 2005 13:11:30 -0500 14, 25 -- MIME-Version: 1.0 14, 25 -- Content-Type: text/plain; 14, 25 -- format=flowed; 14, 25 -- charset="Windows-1252"; 14, 25 -- reply-type=original 14, 25 -- Content-Transfer-Encoding: 8bit 14, 25 -- X-Priority: 3 14, 25 -- X-MSMail-Priority: Normal 14, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 14, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
It's holiday shopping time again. Good books about magnification written for the beginning reader are scarce. If your young one will be getting a dissecting scope or a magnifying glass this year, there are two new small, inexpensive pamphlets that you should consider: "Looking Through a Microscope" & "You can Use a Magnifying Glass". You'll find information on both in the MICRO bibliography (URL below); enter "supplimental books", "for the primary grades", & "recommended" in the search engine. -- Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 1, 15 -- From schooley-at-mcn.org Sun Nov 27 13:56:52 2005 1, 15 -- Received: from dns3.mcn.org (dns3.mcn.org [216.150.240.32]) 1, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jARJupvr024677 1, 15 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sun, 27 Nov 2005 13:56:52 -0600 1, 15 -- Received: from [66.42.18.149] (helo=[10.0.1.2]) 1, 15 -- by dns3.mcn.org with esmtpa (Exim 4.43) 1, 15 -- id IQMQ2Q-000H1D-E1 1, 15 -- for Microscopy-at-MSA.Microscopy.Com; Sun, 27 Nov 2005 11:56:51 -0800 1, 15 -- Mime-Version: 1.0 1, 15 -- Message-Id: {a06200703bfafbc0e28d9-at-[10.0.1.2]} 1, 15 -- Date: Sun, 27 Nov 2005 11:52:59 -0800 1, 15 -- To: Microscopy-at-MSA.Microscopy.Com 1, 15 -- From: Caroline Schooley {schooley-at-mcn.org} 1, 15 -- Subject: [Microscopy] children's microscopy 1, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Surplus Shed (surplusshed.com) has folding doublet 10X magnifiers for $1.25 ea. (or 5/5.00). Glass lenses, aluminum & plastic housing. I like to drill a hole through plastic hinge area to attach a neck string. Great stocking stuffers, party favors, souvenirs of a field trip to your lab, or just carry a few around in your pocket to give to youngsters or leave them in strategic locations for someone to find (random act of kindness?). Price of a candy bar. I'm ordering my second 100 of them. I reckon if it gets one kid in 10 away from the video game or TV for a little while, it's money well spent.
Disclaimer: I have no connection to Surplus Shed; I'm just cheap, and like to pass along a 'steal' when I see one.
Paul
---------------------------------------------------------------------------- The world is so full of a number of things I wonder we're not all as happy as kings
--Robert Louis Stevenson
==============================Original Headers============================== 6, 24 -- From pgrover-at-bilbo.bio.purdue.edu Mon Nov 28 08:20:34 2005 6, 24 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 6, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jASEKYOd006024 6, 24 -- for {microscopy-at-microscopy.org} ; Mon, 28 Nov 2005 08:20:34 -0600 6, 24 -- Received: from paklabpgrover (dhcp155-92.bio.purdue.edu [128.210.155.92]) 6, 24 -- by mailhub128.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id jASEKXXj017266 6, 24 -- for {microscopy-at-microscopy.org} ; Mon, 28 Nov 2005 09:20:34 -0500 6, 24 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu} 6, 24 -- To: {microscopy-at-microscopy.org} 6, 24 -- Subject: $ 1 stocking stuffers 6, 24 -- Date: Mon, 28 Nov 2005 09:20:37 -0500 6, 24 -- Message-ID: {000001c5f426$e7916fd0$5c9bd280-at-paklabpgrover} 6, 24 -- MIME-Version: 1.0 6, 24 -- Content-Type: text/plain; 6, 24 -- charset="us-ascii" 6, 24 -- X-Priority: 3 (Normal) 6, 24 -- X-MSMail-Priority: Normal 6, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.4510 6, 24 -- Importance: Normal 6, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 6, 24 -- X-PMX-Version: 4.7.1.128075 6, 24 -- X-PerlMx-Virus-Scanned: Yes 6, 24 -- Content-Transfer-Encoding: 8bit 6, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jASEKYOd006024 ==============================End of - Headers==============================
***Second announcement--confirmations will start end of December***
We are still looking for some great ideas for symposia at M&M2007!
Microscopy & Microanalysis 2007 Meeting
August 6-9, 2007 Broward County Convention Center Ft. Lauderdale, Florida
Co-sponsored by
The Microscopy Society of America The Microbeam Analysis Society The International Metallographic Society
Proposals are coming in. We have suggestions for some of the customary symposia, but have signed up only four organizers of these. Otherwise, the program is still largely open at this time.
We would like the majority of the proposals to be submitted by the end of the year. We will start sending out acceptance letters in late December, and by mid-February we expect the program to be mostly filled.
This timetable is considerably accelerated in comparison with previous years, and we now require a description of 150-300 words for each proposed symposia. The description should take the form of those found in the Call for Papers and Expo of past years; it should be an announcement of the symposium and an invitation for contributions. The Program Committee will select symposia based on these descriptions, so that overlap will be minimized and symposia will complement each other to form a coherent overall program.
You need not be a member of MSA, MAS, or IMS to propose a symposium, although we hope that your experience with the M&M meeting will encourage you to join.
Please send your suggestion (complete with description) directly to the Program Chair, or to the M&M2007 Co-Chair of your Society.
The M&M2007 website is
http://mm2007.microscopy.org/
Early next year, symposia will be posted on the website as they are accepted.
Program contacts:
Mike Marko, Program Chair (marko-at-wadsworth.org) John Henry Scott, Vice Program Chair (johnhenry.scott-at-nist.gov) Ed Vincenzi, MAS Program Co-Chair (vicenzi-at-volcano.si.edu) Steve Dekanich, IMS Program Co-Chair (dekanichsj-at-y12.doe.gov)
Local Arrangments contact:
Lucille Giannuzzi, Local Arrangements Chair (lgiannuzzi-at-feico.com)
Meeting Management contact:
Phillip Ridley, Conference Manager Bostrom Corp 230 East Ohio, Suite 400 Chicago, IL 60611 Tel: 312-644-0828 Fax: 312-644-8557 Email: pridley-at-bostrom.com
==============================Original Headers============================== 23, 22 -- From marko-at-wadsworth.org Mon Nov 28 09:12:27 2005 23, 22 -- Received: from mailserv.wadsworth.org (mailserv.wadsworth.org [199.184.30.43]) 23, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jASFCRlm015534 23, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Nov 2005 09:12:27 -0600 23, 22 -- Received: from jinmen (pasteur-0253 [172.16.11.253]) 23, 22 -- by mailserv.wadsworth.org (8.12.10+Sun/8.12.10) with SMTP id jASFCOeZ012994 23, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Nov 2005 10:12:24 -0500 (EST) 23, 22 -- Message-Id: {3.0.6.32.20051128103531.00a43c40-at-pop3s.wadsworth.org} 23, 22 -- X-Sender: marko-at-pop3s.wadsworth.org 23, 22 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 23, 22 -- Date: Mon, 28 Nov 2005 10:35:31 -0800 23, 22 -- To: Microscopy-at-microscopy.com 23, 22 -- From: Michael Marko {marko-at-wadsworth.org} 23, 22 -- Subject: Invitation to organize symposia for M&M2007 23, 22 -- Mime-Version: 1.0 23, 22 -- Content-Type: text/plain; charset="us-ascii" 23, 22 -- X-Wadsworth-MailScanner-Information: Please contact CSS for more information 23, 22 -- X-Wadsworth-MailScanner: Found to be clean 23, 22 -- X-Wadsworth-MailScanner-SpamCheck: not spam, SpamAssassin (score=-98.901, 23, 22 -- required 5, AWL -0.86, DATE_IN_FUTURE_03_06 1.96, 23, 22 -- USER_IN_WHITELIST -100.00) 23, 22 -- X-MailScanner-From: marko-at-wadsworth.org ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (morgansbearhunter-at-yahoo.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, November 28, 2005 at 10:49:22 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both morgansbearhunter-at-yahoo.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: Lately when I embed clinical tissue in blocks I get tiny little holes all over the tissue. I changed the dehydration Etoh, the propylene oxide. Now I am wondering if it could be the spurrs NSA. We ordered it and received in 2002. Do you have any suggestions? Kathryn Privett
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both milton.charlton-at-utoronto.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Jena operating scope parts
Question: Could anyone tell me where to get a replacement rack and pinion foucusing mechanism for a Jena operating scope that was made in the 1960's? Thanks M. Charlton University of Toronto
Many thanks to y'all who replied to my question about under/over focus in TEM. I think I have enough of an idea now.
Now for a SEM question. I've seen many questions about grain size of sputtered gold on this list, but what about the tendency of gold films to greak up into a sub-micron "crazy-paving" structure? Does anyone know what causes this, and how to cure it? Often the crazy paving totally dominates the picture, and one has to mentally filter it out to see the underlying structure.
----------------------------------- Robert H. Olley Reply to: R.H.Olley-at-reading.ac.uk URL: http://www.rdg.ac.uk/~spsolley -----------------------------------
==============================Original Headers============================== 6, 21 -- From hinmeigeng-at-hotmail.com Mon Nov 28 14:15:30 2005 6, 21 -- Received: from hotmail.com (bay101-f39.bay101.hotmail.com [64.4.56.49]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jASKFR6p014247 6, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 28 Nov 2005 14:15:29 -0600 6, 21 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 6, 21 -- Mon, 28 Nov 2005 12:15:21 -0800 6, 21 -- Message-ID: {BAY101-F395BB7C8EC9FD2BC1019C2CA480-at-phx.gbl} 6, 21 -- Received: from 64.4.56.200 by by101fd.bay101.hotmail.msn.com with HTTP; 6, 21 -- Mon, 28 Nov 2005 20:15:21 GMT 6, 21 -- X-Originating-IP: [86.128.212.86] 6, 21 -- X-Originating-Email: [hinmeigeng-at-hotmail.com] 6, 21 -- X-Sender: hinmeigeng-at-hotmail.com 6, 21 -- Reply-To: R.H.Olley-at-reading.ac.uk 6, 21 -- From: "Robert H. Olley" {hinmeigeng-at-hotmail.com} 6, 21 -- To: Microscopy-at-MSA.Microscopy.Com 6, 21 -- Cc: R.H.Olley-at-reading.ac.uk 6, 21 -- Subject: Thanks (TEM) + Question (SEM) 6, 21 -- Date: Mon, 28 Nov 2005 20:15:21 +0000 6, 21 -- Mime-Version: 1.0 6, 21 -- Content-Type: text/plain; format=flowed 6, 21 -- X-OriginalArrivalTime: 28 Nov 2005 20:15:21.0496 (UTC) FILETIME=[75770180:01C5F458] ==============================End of - Headers==============================
One (prehaps the major) cause of the "crazy-paving" or "dried river bed" appearance of specimens that were coated with heavy metals for SEM viewing is the expansion and contraction of the underlying specimen. Since the metal coating has little flex/stretch capabilities, it will break (or shatter) if the underlying specimen flexes or expands. The causes of the expansion of the specimen may be: absorption/loss of moisture (as one goes in and out of the vacuum), heating/cooling, mechanical flexing. This can be minimized by keeping the specimens always in a dry environment, at a standard temperature and not bending/flexing them.
} Now for a SEM question. I've seen many questions about grain size of } sputtered gold on this list, but what about the tendency of gold films to } greak up into a sub-micron "crazy-paving" structure? Does anyone know what } causes this, and how to cure it? Often the crazy paving totally dominates } the picture, and one has to mentally filter it out to see the underlying } structure. } } ----------------------------------- } Robert H. Olley } Reply to: R.H.Olley-at-reading.ac.uk } URL: http://www.rdg.ac.uk/~spsolley } -----------------------------------
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==============================Original Headers============================== 4, 18 -- From bozzola-at-siu.edu Mon Nov 28 14:49:32 2005 4, 18 -- Received: from abbmta1.siu.edu (abbmta1.siu.edu [131.230.254.205]) 4, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jASKnWbG023585 4, 18 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 28 Nov 2005 14:49:32 -0600 4, 18 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) 4, 18 -- by abbmta1.siu.edu (Switch-3.1.6/Switch-3.1.0) with ESMTP id jASKnUno003303 4, 18 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 28 Nov 2005 14:49:31 -0600 (CST) 4, 18 -- Mime-Version: 1.0 4, 18 -- X-Sender: bozzola-at-saluki-mail.siu.edu 4, 18 -- Message-Id: {p06110401bfb11c2eeedd-at-[131.230.177.142]} 4, 18 -- In-Reply-To: {200511282019.jASKJ4VV016196-at-ns.microscopy.com} 4, 18 -- References: {200511282019.jASKJ4VV016196-at-ns.microscopy.com} 4, 18 -- Date: Mon, 28 Nov 2005 14:49:29 -0600 4, 18 -- To: Microscopy-at-msa.microscopy.com 4, 18 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 4, 18 -- Subject: Re: [Microscopy] Thanks (TEM) + Question (SEM) 4, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 18 -- X-MASF: 0.00% ==============================End of - Headers==============================
The quick answer to your question concerning the structure of gold coatings is to not use gold coatings. Gold is notorious for nucleating islands on the surface that grow and eventually coalesce to form the coating. This growth pattern results in your "crazy-paving" structure. In fact, the resolution test sample for SEM has traditionally been a gold coating evaporated onto a smooth carbon substrate and the minimum distance between adjacent gold islands is used as the acceptance measurement for the microscope. Au-Pd will offer a better coating on inexpensive desktop coaters than plain gold, but you will still see the grain structure at higher magnifications. Ion Sputter coating systems such as our IBS/e system can sputter numerous different materials. The materials that offer extremely good, uniform, and small structure suitable for high resolution SEM work are Pt, Pd, Cr, Ir, and W.
If you look up Elaine Humphrey's response to a similar question on this listserver from earlier this year, she gives a link to a web site that shows comparisons of coatings from different materials using one of our competitors' systems. (Sorry, I hope you understand why I don't give out the URL.)
-Scott
Disclaimer: South Bay Technology, Inc. manufactures and sells the IBS/e ion beam sputter deposition and etching system.
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: hinmeigeng-at-hotmail.com [mailto:hinmeigeng-at-hotmail.com] Sent: Monday, November 28, 2005 12:23 PM To: Walck-at-SouthBayTech.com
Many thanks to y'all who replied to my question about under/over focus in TEM. I think I have enough of an idea now.
Now for a SEM question. I've seen many questions about grain size of sputtered gold on this list, but what about the tendency of gold films to greak up into a sub-micron "crazy-paving" structure? Does anyone know what causes this, and how to cure it? Often the crazy paving totally dominates the picture, and one has to mentally filter it out to see the underlying
structure.
----------------------------------- Robert H. Olley Reply to: R.H.Olley-at-reading.ac.uk URL: http://www.rdg.ac.uk/~spsolley -----------------------------------
==============================Original Headers============================== 6, 21 -- From hinmeigeng-at-hotmail.com Mon Nov 28 14:15:30 2005 6, 21 -- Received: from hotmail.com (bay101-f39.bay101.hotmail.com [64.4.56.49]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jASKFR6p014247 6, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 28 Nov 2005 14:15:29 -0600 6, 21 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 6, 21 -- Mon, 28 Nov 2005 12:15:21 -0800 6, 21 -- Message-ID: {BAY101-F395BB7C8EC9FD2BC1019C2CA480-at-phx.gbl} 6, 21 -- Received: from 64.4.56.200 by by101fd.bay101.hotmail.msn.com with HTTP; 6, 21 -- Mon, 28 Nov 2005 20:15:21 GMT 6, 21 -- X-Originating-IP: [86.128.212.86] 6, 21 -- X-Originating-Email: [hinmeigeng-at-hotmail.com] 6, 21 -- X-Sender: hinmeigeng-at-hotmail.com 6, 21 -- Reply-To: R.H.Olley-at-reading.ac.uk 6, 21 -- From: "Robert H. Olley" {hinmeigeng-at-hotmail.com} 6, 21 -- To: Microscopy-at-MSA.Microscopy.Com 6, 21 -- Cc: R.H.Olley-at-reading.ac.uk 6, 21 -- Subject: Thanks (TEM) + Question (SEM) 6, 21 -- Date: Mon, 28 Nov 2005 20:15:21 +0000 6, 21 -- Mime-Version: 1.0 6, 21 -- Content-Type: text/plain; format=flowed 6, 21 -- X-OriginalArrivalTime: 28 Nov 2005 20:15:21.0496 (UTC) FILETIME=[75770180:01C5F458] ==============================End of - Headers==============================
==============================Original Headers============================== 19, 24 -- From walck-at-southbaytech.com Mon Nov 28 15:04:37 2005 19, 24 -- Received: from pimout7-ext.prodigy.net (pimout7-ext.prodigy.net [207.115.63.58]) 19, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jASL4aom000333 19, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Nov 2005 15:04:36 -0600 19, 24 -- X-ORBL: [64.169.193.90] 19, 24 -- Received: from dynamicbl8uno3 (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90]) 19, 24 -- by pimout7-ext.prodigy.net (8.13.4 outbound domainkey aix/8.13.4) with ESMTP id jASKvnOS116212; 19, 24 -- Mon, 28 Nov 2005 15:57:54 -0500 19, 24 -- From: "Scott Walck" {walck-at-southbaytech.com} 19, 24 -- To: {Microscopy-at-microscopy.com} 19, 24 -- Cc: {hinmeigeng-at-hotmail.com} 19, 24 -- Subject: RE: [Microscopy] Thanks (TEM) + Question (SEM) 19, 24 -- Date: Mon, 28 Nov 2005 12:58:19 -0800 19, 24 -- Message-ID: {000901c5f45e$7c20e180$7801a8c0-at-dynamicbl8uno3} 19, 24 -- MIME-Version: 1.0 19, 24 -- Content-Type: text/plain; 19, 24 -- charset="us-ascii" 19, 24 -- Content-Transfer-Encoding: 7bit 19, 24 -- X-Priority: 3 (Normal) 19, 24 -- X-MSMail-Priority: Normal 19, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 19, 24 -- Importance: Normal 19, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 19, 24 -- In-Reply-To: {200511282022.jASKMeGc020884-at-ns.microscopy.com} ==============================End of - Headers==============================
Kathryn My guess is, the holes will go away if you use a dehumidifier to bring the relative humidity in your work-room (or work-chamber) below 60-70% before, during and after the last change of 100% accelerated resin. We used to get episodes of holes in thin sections of our Araldite 506-DDSA-DER 736 resin mixture until we followed the advice we found in this paper:
(1977) H. D. Dellman and C. Pearson Better epoxy resin embedding for electron microscopy at low relative humidity. Stain Technol. 52:5-8.
This is essential for us, perhaps moreso than for most labs, because we need a dehumidified environment during the 15-90 minutes it may take us to lovingly manipulate fully infiltrated single muscle fibers and rafts of 3-5 single fibers into position on regions of dry substrate with only a minimal micro-meniscus of external resin; once thus positioned, they stick themselves nicely to each other and to the smooth substrate of polypropylene sheet (by surface tension) during the first 2-4 hours of cure at 60° C. We then invert a full BEEM capsule of liquid resin over the fiber or raft and finish the cure overnight at 80°C. This gives us superbly oriented single fibers exactly parallel to the flat surface of the cured block and lying within 3-5 microns of the resin surface; we like to use it to co-embed fibers from different experiments in a single raft, so we can make one longitudinal (or cross-) section of a such a combi-block do the work of 3-5 sections of separate blocks.
The very high surface-to-volume ratio of this "dry, flat" embedding procedure makes the resin very susceptible to humidity during the pre-cure manipulations. So we go for as low a humidity as our commercial dehumidifier can achieve in our small workroom, often below 50% if we run the thing all night in a closed room. Obviously even lower levels could be rapidly attained if the dehumidified outflow from the appliance were delivered into a small desktop working chamber, but we've not found that to be necessary for our work.
The curing oven itself is not a worry-- its internal RH is below 10%, if i recall our few measurements some years ago. So we typically accelerate infiltration itself in 100% accelerated resin mixture by putting the vials on a sloped rotator in the 60°C oven, and remembering to change the resin for fresh every 30 minutes, x2 or x3. In that heat, the resin mixture becomes so water-thin, and stays that way for at least 40 minutes, that I've long supposed even Spurr's or similar could not become significantly lower in viscosity or penetrate the tissue any more completely.
-mike reedy-
At 11:04 AM -0600 11/28/05, morgansbearhunter-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
On Nov 25, 2005, at 10:46 AM, weis183-at-yahoo.fr wrote:
} A more expensive calibration standard "MAG*I*CAL" is } sold, does someone has experienced it? } Dear Patrick, I have used the MAG*I*CAL, and it works very well. You can calibrate up to the highest mags on your scope using the Si lattice, and you can calibrate camera lengths. If you have a tilt-rotation stage, you can orient the MAG*I*CAL precisely to do all the calibrations, and with a single-tilt stage you will have to orient the grid within the stage for best results. The only problem with it is that it should be used at room temperature, which is not a big deal except for cryo facilities. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Mon Nov 28 17:50:45 2005 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jASNojN4020808 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 28 Nov 2005 17:50:45 -0600 4, 22 -- Received: from localhost (fire-dog [192.168.1.4]) 4, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 638FB33A11 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 28 Nov 2005 15:50:44 -0800 (PST) 4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 4, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id BF64535256 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 28 Nov 2005 15:50:43 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v623) 4, 22 -- In-Reply-To: {200511251846.jAPIkVQ6029917-at-ns.microscopy.com} 4, 22 -- References: {200511251846.jAPIkVQ6029917-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {bf7aaca7abba77473de68387f5743ba6-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] TEM calibration standard 4, 22 -- Date: Mon, 28 Nov 2005 15:55:59 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.623) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.3.2 ==============================End of - Headers==============================
I concur with Bill Tivol on the MAG*I*CAL calibration standard. We have been using it since it first came out several years ago and have been extremely pleased with it as it covers the entire magnification range of the TEM and can be used to calibrate camera lenth, etc.
You need to be very careful with it (not for students to manipulate) since it is brittle and easily broken. Yes, I broke one (a $900 mistake) when I put it back onto the membrane of the storage box. It is best to allow it to drop gently onto the membrane since if you misjudge the exact location of the membrane you can flex and break it.
Go for it and handle with care.
JB
} On Nov 25, 2005, at 10:46 AM, weis183-at-yahoo.fr wrote: } } } A more expensive calibration standard "MAG*I*CAL" is } } sold, does someone has experienced it? } } } Dear Patrick, } I have used the MAG*I*CAL, and it works very well. You can calibrate } up to the highest mags on your scope using the Si lattice, and you can } calibrate camera lengths. If you have a tilt-rotation stage, you can } orient the MAG*I*CAL precisely to do all the calibrations, and with a } single-tilt stage you will have to orient the grid within the stage for } best results. The only problem with it is that it should be used at } room temperature, which is not a big deal except for cryo facilities. } Yours, } Bill Tivol, PhD } EM Scientist and Manager } Cryo-Electron Microscopy Facility } Broad Center, Mail Code 114-96 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu } } } ==============================Original Headers============================== } 4, 22 -- From tivol-at-caltech.edu Mon Nov 28 17:50:45 2005 } 4, 22 -- Received: from outgoing-mail.its.caltech.edu } (outgoing-mail.its.caltech.edu [131.215.239.19]) } 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } jASNojN4020808 } 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 28 Nov 2005 } 17:50:45 -0600 } 4, 22 -- Received: from localhost (fire-dog [192.168.1.4]) } 4, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 638FB33A11 } 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 28 Nov 2005 } 15:50:44 -0800 (PST) } 4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) } 4, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id BF64535256 } 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 28 Nov 2005 } 15:50:43 -0800 (PST) } 4, 22 -- Mime-Version: 1.0 (Apple Message framework v623) } 4, 22 -- In-Reply-To: {200511251846.jAPIkVQ6029917-at-ns.microscopy.com} } 4, 22 -- References: {200511251846.jAPIkVQ6029917-at-ns.microscopy.com} } 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed } 4, 22 -- Message-Id: {bf7aaca7abba77473de68387f5743ba6-at-caltech.edu} } 4, 22 -- Content-Transfer-Encoding: 7bit } 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} } 4, 22 -- Subject: Re: [Microscopy] TEM calibration standard } 4, 22 -- Date: Mon, 28 Nov 2005 15:55:59 -0800 } 4, 22 -- To: microscopy-at-msa.microscopy.com } 4, 22 -- X-Mailer: Apple Mail (2.623) } 4, 22 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.3.2 } ==============================End of - Headers==============================
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==============================Original Headers============================== 10, 18 -- From bozzola-at-siu.edu Mon Nov 28 19:52:06 2005 10, 18 -- Received: from abbmta1.siu.edu (abbmta1.siu.edu [131.230.254.205]) 10, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAT1q6vh031052 10, 18 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 28 Nov 2005 19:52:06 -0600 10, 18 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) 10, 18 -- by abbmta1.siu.edu (Switch-3.1.6/Switch-3.1.0) with ESMTP id jAT1q4tC001730 10, 18 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 28 Nov 2005 19:52:05 -0600 (CST) 10, 18 -- Mime-Version: 1.0 10, 18 -- X-Sender: bozzola-at-saluki-mail.siu.edu 10, 18 -- Message-Id: {p06110404bfb163489911-at-[131.230.177.142]} 10, 18 -- In-Reply-To: {200511282351.jASNpkGF022709-at-ns.microscopy.com} 10, 18 -- References: {200511282351.jASNpkGF022709-at-ns.microscopy.com} 10, 18 -- Date: Mon, 28 Nov 2005 19:52:04 -0600 10, 18 -- To: Microscopy-at-msa.microscopy.com 10, 18 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 10, 18 -- Subject: Re: TEM calibration standard 10, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 10, 18 -- X-MASF: 0.00% ==============================End of - Headers==============================
Robert H. Olley wrote: ======================================================== Now for a SEM question. I've seen many questions about grain size of sputtered gold on this list, but what about the tendency of gold films to greak up into a sub-micron "crazy-paving" structure? Does anyone know what causes this, and how to cure it? Often the crazy paving totally dominates the picture, and one has to mentally filter it out to see the underlying structure. ======================================================== Our philosophy has always been that if this is happening, then you are probably putting on too much gold and/or exposing the sample to too much heat. Other factors can also increase the chances of seeing this kind of effect, for example, the presence of a thin lubricant coating such as on a storage media surface, catheter tubing or syringe needles. Additionally, certain materials like PTFE are especially prone to this kind of cracking. I have always assumed that this was at least in part due to the inherent "grain" structure of any sputtered coating.
A layer of osmium metal deposited in an OPC osmium plasma coater, see URL http://www.2spi.com/catalog/osmi-coat.html which is not a sputtered coating, has no grain size (at least no one has detected a grain size to our knowledge) and seems to be much more resistant to the "crazy-paving" structure pattern effect on the above mentioned samples. A comparison between different coating materials vs. osmium metal is shown on URL http://www.2spi.com/catalog/comparison-coating-results.html I propose that the osmium coating approach would qualify for the requested "cure".
Disclaimer: SPI Supplies is the worldwide distributor of the OPC line of osmium plasma coaters outside of Japan so we would naturally have a vested interest in promoting the use of the osmium coaters for SEM sample preparation.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
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==============================Original Headers============================== 12, 25 -- From cgarber-at-2spi.com Mon Nov 28 23:00:33 2005 12, 25 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 12, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAT50Wxh009515 12, 25 -- for {microscopy-at-msa.microscopy.com} ; Mon, 28 Nov 2005 23:00:33 -0600 12, 25 -- Received: from ibm1x23g2abfyg ([68.246.124.205]) 12, 25 -- by diskless5.axs2000.net (8.12.11/8.12.11) with SMTP id jAT50KqV005391; 12, 25 -- Tue, 29 Nov 2005 00:00:27 -0500 12, 25 -- X-IDV-FirstRcvd: [68.246.124.205] 12, 25 -- X-IDV-HELO: ibm1x23g2abfyg 12, 25 -- Message-ID: {010d01c5f4a1$cd9d5780$cd7cf644-at-ibm1x23g2abfyg} 12, 25 -- Reply-To: "Garber, Charles A." {cgarber-at-2spi.com} 12, 25 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 12, 25 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 12, 25 -- Subject: Crazy paving structures in gold sputtered films 12, 25 -- Date: Mon, 28 Nov 2005 23:59:36 -0500 12, 25 -- MIME-Version: 1.0 12, 25 -- Content-Type: text/plain; 12, 25 -- format=flowed; 12, 25 -- charset="Windows-1252"; 12, 25 -- reply-type=original 12, 25 -- Content-Transfer-Encoding: 7bit 12, 25 -- X-Priority: 3 12, 25 -- X-MSMail-Priority: Normal 12, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 12, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
I've seen this "spider man" appearance before and attribute it to sputtering at too high of pressure and too high of current. Au is not a great metal for high resolution SEM anyway...IMO. Au/Pd is better, and then consider Pt or Ir.
But it seems to me that low vacuum (15mT) and low current (9mA) makes a big difference. But you will not likely get 15mT or lower without a turbo pumped system.
Try your system at the lowest vacuum you can get and see what happens.
gary g.
At 12:20 PM 11/28/2005, you wrote:
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==============================Original Headers============================== 10, 24 -- From gary-at-gaugler.com Mon Nov 28 23:35:03 2005 10, 24 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jAT5Z2pG018811 10, 24 -- for {microscopy-at-microscopy.com} ; Mon, 28 Nov 2005 23:35:02 -0600 10, 24 -- Received: (qmail 28909 invoked from network); 28 Nov 2005 21:34:17 -0800 10, 24 -- Received: by simscan 1.1.0 ppid: 28900, pid: 28901, t: 5.4761s 10, 24 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1177 spam: 3.0.3 10, 24 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 24 -- by qsmtp3 with SMTP; 28 Nov 2005 21:34:11 -0800 10, 24 -- Message-Id: {6.2.3.4.2.20051128212957.02913530-at-mail.calweb.com} 10, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 10, 24 -- Date: Mon, 28 Nov 2005 21:35:00 -0800 10, 24 -- To: hinmeigeng-at-hotmail.com 10, 24 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 24 -- Subject: Re: [Microscopy] Thanks (TEM) + Question (SEM) 10, 24 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 24 -- In-Reply-To: {200511282020.jASKK7wY017560-at-ns.microscopy.com} 10, 24 -- References: {200511282020.jASKK7wY017560-at-ns.microscopy.com} 10, 24 -- Mime-Version: 1.0 10, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 10, 24 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp3.surewest.net 10, 24 -- X-Spam-Level: 10, 24 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 10, 24 -- version=3.0.3 ==============================End of - Headers==============================
Hi, The 1991 BJ item is a meeting abstract, not article. It did cite the prior work that inspired extensive trials we made of these methods in the late 1980s.
Today I scanned it and a companion abstract about TAOS and TAURAC fixation into a PDF and will separately email to you directly (no idea how or if to transmit attachments via ListServer). My Acrobat 6 OCR function refuses to produce searchable text on these so they are just images.; if i get an OCR to work, I will send that later.
David Popp and I planned to write up a methods paper with some results but never finished the effort. We found that TAURAC-fixed Araldite-embedded fibers of permeabilized, insect flight muscle (fixed by TAURAC with a glutaraldehyde fixation interposed between the TA and the UrAc fixes) still give fiber x-ray patterns showing axial reflections out to 1.3 nm, and this result we will report in a paper soon to be submitted by Kasim Sader (Univ. Leeds) et al.
The method can be cited from a 1994 peer-reviewed paper by H. Schmitz et al., where it was described and some results illustrated in Biophysical Journal (67:1620-1633).
-mike reedy-
At 10:26 AM -0600 11/29/05, Tindall, Randy D. wrote: } Hi, } } I read this great post with great interest and have succeeded in } tracking down two of the refs. I have been unable to locate the 1991 } Biophysical Journal article, however. Is the reference correct? Do you } maybe have a PDF of this you could share? We have a client who is } always looking for nifty ways to preserve biofilms and such. } } Thanks and Happy Holidays, } Randy } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W122 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } } } } -----Original Message----- } From: mike.reedy-at-cellbio.duke.edu [mailto:mike.reedy-at-cellbio.duke.edu] } Sent: Thursday, November 17, 2005 1:30 AM } To: Tindall, Randy D. } Subject: [Microscopy] Re: SEM imaging of bacteria encapsulation } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
I am doing some research with Tungsten Oxide. I got WO2.9 phase which is tetragonal phase with space group of P4/nmm (from XRD). I wonder if anybody have the atomic positions and Wykoff notations for this phase. I have searched the literature but no luck. Thank You
Jafar
==============================Original Headers============================== 5, 33 -- From jafarhan-at-rci.rutgers.edu Tue Nov 29 20:32:44 2005 5, 33 -- Received: from annwn1.rutgers.edu (nbcs-av.rutgers.edu [128.6.72.254]) 5, 33 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAU2WiH6003775 5, 33 -- for {microscopy-at-microscopy.com} ; Tue, 29 Nov 2005 20:32:44 -0600 5, 33 -- Received: from localhost (localhost.rutgers.edu [127.0.0.1]) 5, 33 -- by annwn1.rutgers.edu (Postfix) with ESMTP id 30022447A2 5, 33 -- for {microscopy-at-microscopy.com} ; Tue, 29 Nov 2005 21:32:42 -0500 (EST) 5, 33 -- Received: from annwn1.rutgers.edu ([127.0.0.1]) 5, 33 -- by localhost (annwn1.rutgers.edu [127.0.0.1]) (amavisd-new, port 10024) 5, 33 -- with ESMTP id 23890-07 for {microscopy-at-microscopy.com} ; 5, 33 -- Tue, 29 Nov 2005 21:32:42 -0500 (EST) 5, 33 -- Received: from rci.rutgers.edu (gehenna.rutgers.edu [128.6.72.72]) 5, 33 -- by annwn1.rutgers.edu (Postfix) with ESMTP id 06963447A0 5, 33 -- for {microscopy-at-microscopy.com} ; Tue, 29 Nov 2005 21:32:42 -0500 (EST) 5, 33 -- Received: from webmail.rci.rutgers.edu (gehenna.rutgers.edu [128.6.72.72]) 5, 33 -- by rci.rutgers.edu (Postfix) with ESMTP id 14C4710DA 5, 33 -- for {microscopy-at-microscopy.com} ; Tue, 29 Nov 2005 21:32:44 -0500 (EST) 5, 33 -- Received: from 172.23.41.187 5, 33 -- (SquirrelMail authenticated user jafarhan) 5, 33 -- by webmail.rci.rutgers.edu with HTTP; 5, 33 -- Tue, 29 Nov 2005 21:32:44 -0500 (EST) 5, 33 -- Message-ID: {37426.172.23.41.187.1133317964.squirrel-at-webmail.rci.rutgers.edu} 5, 33 -- Date: Tue, 29 Nov 2005 21:32:44 -0500 (EST) 5, 33 -- Subject: Atomic positions for WO2.9 5, 33 -- From: jafarhan-at-rci.rutgers.edu 5, 33 -- To: microscopy-at-microscopy.com 5, 33 -- User-Agent: SquirrelMail/1.4.5 5, 33 -- MIME-Version: 1.0 5, 33 -- Content-Type: text/plain;charset=iso-8859-1 5, 33 -- Content-Transfer-Encoding: 8bit 5, 33 -- X-Priority: 3 (Normal) 5, 33 -- Importance: Normal 5, 33 -- X-Virus-Scanned: Virus Scanned by NBCS ==============================End of - Headers==============================
I know that there are a number of WO3 and WO(3-x) compounds in the PDF database from when I worked on the oxidation of WS2 thin films. Have you checked whether this space group agrees with the WO3 phase? If it does, then the positions are the same and you have to think of the x=.1 as oxygen vacancies that are distributed through the material.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: jafarhan-at-rci.rutgers.edu [mailto:jafarhan-at-rci.rutgers.edu] Sent: Tuesday, November 29, 2005 6:37 PM To: Walck-at-SouthBayTech.com
Dear All,
I am doing some research with Tungsten Oxide. I got WO2.9 phase which is tetragonal phase with space group of P4/nmm (from XRD). I wonder if anybody have the atomic positions and Wykoff notations for this phase. I have searched the literature but no luck. Thank You
Jafar
==============================Original Headers============================== 5, 33 -- From jafarhan-at-rci.rutgers.edu Tue Nov 29 20:32:44 2005 5, 33 -- Received: from annwn1.rutgers.edu (nbcs-av.rutgers.edu [128.6.72.254]) 5, 33 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAU2WiH6003775 5, 33 -- for {microscopy-at-microscopy.com} ; Tue, 29 Nov 2005 20:32:44 -0600 5, 33 -- Received: from localhost (localhost.rutgers.edu [127.0.0.1]) 5, 33 -- by annwn1.rutgers.edu (Postfix) with ESMTP id 30022447A2 5, 33 -- for {microscopy-at-microscopy.com} ; Tue, 29 Nov 2005 21:32:42 -0500 (EST) 5, 33 -- Received: from annwn1.rutgers.edu ([127.0.0.1]) 5, 33 -- by localhost (annwn1.rutgers.edu [127.0.0.1]) (amavisd-new, port 10024) 5, 33 -- with ESMTP id 23890-07 for {microscopy-at-microscopy.com} ; 5, 33 -- Tue, 29 Nov 2005 21:32:42 -0500 (EST) 5, 33 -- Received: from rci.rutgers.edu (gehenna.rutgers.edu [128.6.72.72]) 5, 33 -- by annwn1.rutgers.edu (Postfix) with ESMTP id 06963447A0 5, 33 -- for {microscopy-at-microscopy.com} ; Tue, 29 Nov 2005 21:32:42 -0500 (EST) 5, 33 -- Received: from webmail.rci.rutgers.edu (gehenna.rutgers.edu [128.6.72.72]) 5, 33 -- by rci.rutgers.edu (Postfix) with ESMTP id 14C4710DA 5, 33 -- for {microscopy-at-microscopy.com} ; Tue, 29 Nov 2005 21:32:44 -0500 (EST) 5, 33 -- Received: from 172.23.41.187 5, 33 -- (SquirrelMail authenticated user jafarhan) 5, 33 -- by webmail.rci.rutgers.edu with HTTP; 5, 33 -- Tue, 29 Nov 2005 21:32:44 -0500 (EST) 5, 33 -- Message-ID: {37426.172.23.41.187.1133317964.squirrel-at-webmail.rci.rutgers.edu} 5, 33 -- Date: Tue, 29 Nov 2005 21:32:44 -0500 (EST) 5, 33 -- Subject: Atomic positions for WO2.9 5, 33 -- From: jafarhan-at-rci.rutgers.edu 5, 33 -- To: microscopy-at-microscopy.com 5, 33 -- User-Agent: SquirrelMail/1.4.5 5, 33 -- MIME-Version: 1.0 5, 33 -- Content-Type: text/plain;charset=iso-8859-1 5, 33 -- Content-Transfer-Encoding: 8bit 5, 33 -- X-Priority: 3 (Normal) 5, 33 -- Importance: Normal 5, 33 -- X-Virus-Scanned: Virus Scanned by NBCS ==============================End of - Headers==============================
==============================Original Headers============================== 15, 24 -- From walck-at-southbaytech.com Wed Nov 30 10:54:49 2005 15, 24 -- Received: from pimout5-ext.prodigy.net (pimout5-ext.prodigy.net [207.115.63.73]) 15, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jAUGsn8Z007872 15, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Nov 2005 10:54:49 -0600 15, 24 -- X-ORBL: [64.169.193.90] 15, 24 -- Received: from dynamicbl8uno3 (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90]) 15, 24 -- by pimout5-ext.prodigy.net (8.13.4 outbound domainkey aix/8.13.4) with ESMTP id jAUGqxs8088268; 15, 24 -- Wed, 30 Nov 2005 11:53:05 -0500 15, 24 -- From: "Scott Walck" {walck-at-southbaytech.com} 15, 24 -- To: {Microscopy-at-microscopy.com} 15, 24 -- Cc: {jafarhan-at-rci.rutgers.edu} 15, 24 -- Subject: RE: [Microscopy] Atomic positions for WO2.9 15, 24 -- Date: Wed, 30 Nov 2005 08:53:03 -0800 15, 24 -- Message-ID: {004901c5f5ce$8dcdf3c0$7801a8c0-at-dynamicbl8uno3} 15, 24 -- MIME-Version: 1.0 15, 24 -- Content-Type: text/plain; 15, 24 -- charset="US-ASCII" 15, 24 -- Content-Transfer-Encoding: 7bit 15, 24 -- X-Priority: 3 (Normal) 15, 24 -- X-MSMail-Priority: Normal 15, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 15, 24 -- Importance: Normal 15, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 15, 24 -- In-Reply-To: {200511300237.jAU2b7Os011591-at-ns.microscopy.com} ==============================End of - Headers==============================
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Email: mczech-at-sjm.com Name: Mike Czech
Organization: St. Jude Medical, Inc
Title-Subject: [Filtered] Eye Damage by Light intensity
Question: Are there any studies that relate light intensity to eye damage from long term microscope usage?
I have an unusual question, I will try to explain it as best I can.
A researcher has asked me to find out if it is reasonable to do anything like a quantitative comparison between fluorescence images of different samples.
She has thick sections, 20 um, on a confocal scope. She sees differences by eye between control and treatments, and she would like to quantify these differences in some way.
We have thought about setting up the confocal to record the brightest image, then without changing the settings, record an image of the other slides. The idea would be that she could do something like compare the brightness levels between them.
I don't know enough about other options or if this will even work to help her.
Does anyone do anything like this or is this not realistic.
Thanks
Jon
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-cats.ucsc.edu
==============================Original Headers============================== 11, 21 -- From jmkrupp-at-cats.ucsc.edu Wed Nov 30 18:36:09 2005 11, 21 -- Received: from cats-mx1.ucsc.edu (cats-mx1.ucsc.edu [128.114.125.34]) 11, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB10a8eu012422 11, 21 -- for {microscopy-at-microscopy.com} ; Wed, 30 Nov 2005 18:36:08 -0600 11, 21 -- Received: from [128.114.25.151] (dhcp-25-151.ucsc.edu [128.114.25.151]) 11, 21 -- by cats-mx1.ucsc.edu (8.13.1/8.13.1) with SMTP id jB10YHAJ002955 11, 21 -- for {microscopy-at-microscopy.com} ; Wed, 30 Nov 2005 16:34:21 -0800 (PST) 11, 21 -- X-Sender: jmkrupp-at-cruzmail.ucsc.edu 11, 21 -- Message-Id: {v01550100bfb3f2222c9e-at-[128.114.25.135]} 11, 21 -- Mime-Version: 1.0 11, 21 -- Content-Type: text/plain; charset="us-ascii" 11, 21 -- Date: Wed, 30 Nov 2005 16:29:42 -0800 11, 21 -- To: microscopy-at-microscopy.com 11, 21 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp) 11, 21 -- Subject: comparing images 11, 21 -- X-UCSC-CATS-Information: This message was scanned by the ITS MailScanner 11, 21 -- X-UCSC-CATS-MailScanner: Found to be clean 11, 21 -- X-UCSC-CATS-MailScanner-SpamCheck: not spam, spamassassin (score=1.57, 11, 21 -- required 8, autolearn=disabled, MISSING_SUBJECT 1.57) 11, 21 -- X-UCSC-CATS-MailScanner-SpamScore: s 11, 21 -- X-UCSC-CATS-MailScanner-From: jmkrupp-at-cats.ucsc.edu ==============================End of - Headers==============================
I'm soliciting the opinion of practicing SEM users regarding the materials to use for a proposed high-precision 200 to 300 nm two-dimensional pitch standard. The standard would be used to calibrate the horizontal scale (X and Y axes) of a SEM or AFM. As I am an AFM expert, I already know what I want in terms of the 3Dimension topography: the specimen will consist of an array of bumps, with height probably 30-70 nm. We are considering using etched silicon, silicon oxide on silicon, or silicon nitride. Do any of these materials have advantages or disadvantages for SEM use? Are some materials ok for low voltage ( { 1kV) and not ok for high voltage? Would it be a good idea to apply a metal coating? It seems obvious to me that all magnetic materials should be avoided, such as Ni, Fe, Cr, Co. Are there additional materials that are forbidden in some environments? For example, I have heard that certain other metals are not allowed in some IC fabs, but I don't know what they are.
I will be grateful to receive your input either via the list (which should stimulate some good discussion) or offline.
regards, Don Chernoff ================================== Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.]
==============================Original Headers============================== 5, 29 -- From donc-at-asmicro.com Wed Nov 30 22:27:31 2005 5, 29 -- Received: from s-utl01-dcpop.stsn.net (s-utl01-dcpop.stsn.net [72.255.0.17]) 5, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jB14RUcP016164 5, 29 -- for {microscopy-at-microscopy.com} ; Wed, 30 Nov 2005 22:27:31 -0600 5, 29 -- Received: from s-utl01-dcpop.stsn.net ([127.0.0.1]) 5, 29 -- by s-utl01-dcpop.stsn.net (SMSSMTP 4.1.2.20) with SMTP id M2005113023272626051 5, 29 -- for {microscopy-at-microscopy.com} ; Wed, 30 Nov 2005 23:27:26 -0500 5, 29 -- X-Spam-Status: No, hits=0.0 required=9.9 5, 29 -- tests=ALL_TRUSTED: -2.867,AWL: 0.306,BAYES_00: -1.665, 5, 29 -- SARE_RECV_ADDR: 0.027 5, 29 -- X-Spam-Level: 5, 29 -- Received: from ASM11 ([10.6.60.244]) 5, 29 -- by s-utl01-dcpop.stsn.net 5, 29 -- for microscopy-at-microscopy.com; 5, 29 -- Wed, 30 Nov 2005 23:27:25 -0500 5, 29 -- Message-ID: {004001c5f62f$80627880$c901a8c0-at-ASM11} 5, 29 -- Reply-To: "Don Chernoff at ASM" {donc-at-asmicro.com} 5, 29 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 5, 29 -- To: "Microscopy List" {microscopy-at-microscopy.com} 5, 29 -- Subject: SEM calibration standard 5, 29 -- Date: Wed, 30 Nov 2005 23:22:00 -0500 5, 29 -- MIME-Version: 1.0 5, 29 -- Content-Type: text/plain; 5, 29 -- charset="iso-8859-1" 5, 29 -- Content-Transfer-Encoding: 7bit 5, 29 -- X-Priority: 3 5, 29 -- X-MSMail-Priority: Normal 5, 29 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1506 5, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1506 ==============================End of - Headers==============================
Look at the Geller 3 and 4 standards. They ought to do what you need. If not, ask them for something else.
gary g.
At 08:47 PM 11/30/2005, you wrote:
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==============================Original Headers============================== 9, 24 -- From gary-at-gaugler.com Wed Nov 30 22:54:08 2005 9, 24 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jB14s76j023632 9, 24 -- for {microscopy-at-microscopy.com} ; Wed, 30 Nov 2005 22:54:07 -0600 9, 24 -- Received: (qmail 25356 invoked from network); 30 Nov 2005 20:52:57 -0800 9, 24 -- Received: by simscan 1.1.0 ppid: 25339, pid: 25340, t: 2.9264s 9, 24 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1142 spam: 3.0.3 9, 24 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 9, 24 -- by qsmtp2 with SMTP; 30 Nov 2005 20:52:54 -0800 9, 24 -- Message-Id: {6.2.3.4.2.20051130205226.028b2900-at-mail.calweb.com} 9, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 9, 24 -- Date: Wed, 30 Nov 2005 20:54:04 -0800 9, 24 -- To: donc-at-asmicro.com 9, 24 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 24 -- Subject: Re: [Microscopy] SEM calibration standard 9, 24 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 24 -- In-Reply-To: {200512010447.jB14lpg3021843-at-ns.microscopy.com} 9, 24 -- References: {200512010447.jB14lpg3021843-at-ns.microscopy.com} 9, 24 -- Mime-Version: 1.0 9, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 9, 24 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp2.surewest.net 9, 24 -- X-Spam-Level: 9, 24 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 9, 24 -- version=3.0.3 ==============================End of - Headers==============================
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