Thanks for all answers about the MAG*I*CAL calibration standard. It seems that it is worth the value even if it must be handled with care (like most TEM thin sections eventually).
Patrick Weisbecker LCTS PESSAC/FRANCE
___________________________________________________________________________ Appel audio GRATUIT partout dans le monde avec le nouveau Yahoo! Messenger Téléchargez cette version sur http://fr.messenger.yahoo.com
==============================Original Headers============================== 6, 18 -- From weis183-at-yahoo.fr Thu Dec 1 01:32:49 2005 6, 18 -- Received: from web26704.mail.ukl.yahoo.com (web26704.mail.ukl.yahoo.com [217.146.176.67]) 6, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jB17WlLU023461 6, 18 -- for {microscopy-at-microscopy.com} ; Thu, 1 Dec 2005 01:32:48 -0600 6, 18 -- Received: (qmail 40705 invoked by uid 60001); 1 Dec 2005 07:32:47 -0000 6, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 18 -- s=s1024; d=yahoo.fr; 6, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 6, 18 -- b=o8gxr867bjO/bErfKTlQPu0qNwcSkqAzln3z39umFpAf04ceA+ajX+0popzy0ndRA2OizPNcDN3Dywp2FOv8vwKaMIf6CcdDEr/bJNtoR8kGpSJBrWkOCNzv9thhyI3SpwLE3fh/9kEwMLnuiq/nA4o7ABkUFOlGfy5QdsV3nis= ; 6, 18 -- Message-ID: {20051201073247.40703.qmail-at-web26704.mail.ukl.yahoo.com} 6, 18 -- Received: from [147.210.80.22] by web26704.mail.ukl.yahoo.com via HTTP; Thu, 01 Dec 2005 08:32:47 CET 6, 18 -- Date: Thu, 1 Dec 2005 08:32:47 +0100 (CET) 6, 18 -- From: Patrick Weisbecker {weis183-at-yahoo.fr} 6, 18 -- Subject: Thanks - TEM calibration standard 6, 18 -- To: microscopy-at-microscopy.com 6, 18 -- MIME-Version: 1.0 6, 18 -- Content-Type: text/plain; charset=iso-8859-1 6, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Hi Jon, the approach you describe to this problem would be sound if all other factors were controlled and, in fact, we use it sometimes. The problem with quantification of fluorescence is that there are so many parameters to control and there is the non-linearity in fluorescence emission to consider, i.e. is emission intensity linearly related to the amount of fluorochrome present? Assuming that physical parameters (preparation technique, section thickness, stain concentration / fluorescent protein expression levels etc.) are controlled, you can fairly easily say that one specimen is dimmer than another. One of the most important caveats is to ensure that the emission levels in the brighter image do not exceed 255. If they do, then you can not reasonably say how bright the brightest point really was. Use a 'glow' palette in the confocal software when setting gain levels to ensure that the brightest specimen levels remain just below saturation. What do you do next? The real trick is to describe why one specimen is brighter than another. Either it will have to do with the relative amounts of the stained tissue or the relative levels of gene expression. Both developmental problems that can be solved genetically or biochemically.
Good luck, John.
jmkrupp-at-cats.ucsc.edu wrote:
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********************************* C. John Runions, Ph.D. School of Biological and Molecular Sciences Oxford Brookes University Oxford, UK OX3 0BP
One way that you can do this is to use the Fluor ref slides to set your system to a specific intensity. If you have even a simple image analysis system, you should be able to take a point intensity reading. I'd recommend that you take it in the middle of the field since lamp to be consistent, since lamp alignment can have a big impact on eveness of illumination across the field.
The slides come in four different spectral responses, so you can probably find one close to the dye your colleague is using.
We have these slides at MME. You can arrange for purchase by contacting Ken Piel at (972)954-8011.
Hope this is helpful
Best regards, Barbara Foster
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P. S. Need a good general reference or light microscopy text for the Spring semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 07:03 PM 11/30/2005, jmkrupp-at-cats.ucsc.edu wrote:
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==============================Original Headers============================== 17, 18 -- From bfoster-at-mme1.com Thu Dec 1 03:40:38 2005 17, 18 -- Received: from 5starpro.com (enterprise.5starpro.com [207.44.136.95]) 17, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB19ea0P019787 17, 18 -- for {microscopy-at-microscopy.com} ; Thu, 1 Dec 2005 03:40:36 -0600 17, 18 -- Received: (qmail 26156 invoked from network); 1 Dec 2005 03:42:44 -0600 17, 18 -- Received: from unknown (HELO barbsd505.mme1.com) (66.153.100.53) 17, 18 -- by enterprise.5starpro.com with SMTP; 1 Dec 2005 03:42:44 -0600 17, 18 -- Message-Id: {6.1.2.0.0.20051201033548.102ebe58-at-mail.mme1.com} 17, 18 -- X-Sender: bfoster-at-mail.mme1.com 17, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 6.1.2.0 17, 18 -- Date: Thu, 01 Dec 2005 03:40:00 -0600 17, 18 -- To: jmkrupp-at-cats.ucsc.edu, microscopy-at-microscopy.com 17, 18 -- From: Barbara Foster {bfoster-at-mme1.com} 17, 18 -- Subject: Re: [Microscopy] comparing images 17, 18 -- In-Reply-To: {200512010103.jB113Zag019885-at-ns.microscopy.com} 17, 18 -- References: {200512010103.jB113Zag019885-at-ns.microscopy.com} 17, 18 -- Mime-Version: 1.0 17, 18 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
In theory measurement of the mean grey level of the relevant image region should give a guide to the concentration of the fluorochrome marker and hence what it is labelled to. Likewise total pixel brightness (sum of all pixel grey levels within the region of interest) should give a guide to the total mass of the fluorochrome marker and hence what it is labelled to. In practice there may be differences in labelling efficiency, plus the fluorescence may be quenched or bleached differently within the two samples, so it's not an exact measurement. You would have to maximise the threshold over the entire region of interest to include the darker areas, and selection of the region of interest can bias the results if you aren't careful. Also you could measure the total area of the pixels greater than a set grey level (thresholded) to give a bit more info on differences.
The freeware Image J (Windows) or its Mac sister NIHImage http://rsb.info.nih.gov/ij/ will do this for you, provided your images are converted to standard TIF etc..formats it can read, although you may need a few optional plug-ins (I use metaMorph and ImageProPlus here but they are many £1,000s per licence). You would need to measure twenty of so different images per group and do some t-test type stats to assess if there is any significant difference (using Excels Stats add-ins). Naturally avoid any post-capture image processing like brightness adjustment, sharpen etc.., and the images should be collected at exactly the same confocal settings for each fluorochrome - although confocal laser power, hardware and optics may drift a little over time so try and take the alternate measurements of control v exposed at the same sittings. The confocal pin-hole size and where you take the z slice within the specimen can naturally also be important.
You can get involved with optical density, but that's really used with densitometry measurements of light passing through tissue, and for that to work you need densimetric metric standards of known density to calibrate the transmission measurements (e.g. bone tissue sections with say polypropylene and aluminium disks etc..). It's difficult to get fluorescence standards to do a similar job for confocal fluorescence. Some suggest uranium glass slides to check if the image capture is 'linear' across the field of view (if you have one from the good old days). With regard to fluorescence calibration standards, Molecular Probes make some fluorescent gel standards that appear to suite. Alternatively you could try submicron fluorescent beads at various known concentrations in gels or similar mountant. I use Mattek dishes (a Petri dish with a hole cut-out and coverslip) with inverted microscopes, which is a bit easier than using slides for these standards. These standards can help check the 'linearity' of the confocal PMT detection system.
Regards Keith
PS. If you can have a look at Joece Loebl's Image Analysis - Principles and Practice. They made the Magiscan B&W and colour Image analysers (before Applied Imaging took them over) during the 1980's and early 1990's. It's long out of print so its library loan only, but it is very clearly written without to many equations.
----- Original Message ----- X-from: {jmkrupp-at-cats.ucsc.edu} To: {keith.morris-at-ucl.ac.uk} Sent: Thursday, December 01, 2005 1:26 AM
We have a Hitichi H500 that we are decommissioning. My question(s) is how much oil is in the high tension tank and also does anyone still use one and would they be interested in parts? Please reply to me via list server or nicholls-at-post.queensu.ca
Rod Nicholls Histology/Electron Microscopy Technician Department of Anatomy and Cell Biology Queen's University Kingston, Ontario K7L 3N6 Phone: 613- 533 6000 x 78265
==============================Original Headers============================== 3, 15 -- From nicholls-at-post.queensu.ca Thu Dec 1 08:33:19 2005 3, 15 -- Received: from post.queensu.ca (post.QueensU.CA [130.15.126.6]) 3, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB1EWvVr029660 3, 15 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Dec 2005 08:33:13 -0600 3, 15 -- Received: from EMSuite.post.queensu.ca (U55.N149.QueensU.CA [130.15.149.55]) 3, 15 -- by post.queensu.ca (8.13.1/8.13.1) with ESMTP id jB1EWvYv012180 3, 15 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Dec 2005 09:32:57 -0500 (EST) 3, 15 -- Message-Id: {6.2.3.4.0.20051201092602.01d04010-at-post.queensu.ca} 3, 15 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 3, 15 -- Date: Thu, 01 Dec 2005 09:32:51 -0500 3, 15 -- To: Microscopy-at-microscopy.com 3, 15 -- From: Rod Nicholls {nicholls-at-post.queensu.ca} 3, 15 -- Subject: Hitachi H500 TEM decommisioning 3, 15 -- Mime-Version: 1.0 3, 15 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Any comparison between images is complicated, as in most cases the acquisition conditions are not or can not be controlled so as to give you identical conditions. This is even more difficult in fluorescence images, as the flourescence can bleach over time, so not only the conditions, but also the timing must be right.
The first problem you can try to tackle by embedding some standards into the preparation. Perhaps some small fluorescent beads that you can then use to normalize the image. Instead of using the intensity itself, you would use the intensity ratio of sample/bead as a measure.
If your samples bleach, you can try to do severl things:
1) acquire a time series of each sample and measure the decay of the signal and extrapolate to time 0, or 2) acquire the image at precise times, for example 1 minute after turning on the illumination.
Good luck.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu] Sent: Wednesday, November 30, 2005 6:06 PM To: Mike Bode
I have an unusual question, I will try to explain it as best I can.
A researcher has asked me to find out if it is reasonable to do anything like a quantitative comparison between fluorescence images of different samples.
She has thick sections, 20 um, on a confocal scope. She sees differences by eye between control and treatments, and she would like to quantify these differences in some way.
We have thought about setting up the confocal to record the brightest image, then without changing the settings, record an image of the other slides. The idea would be that she could do something like compare the brightness levels between them.
I don't know enough about other options or if this will even work to help her.
Does anyone do anything like this or is this not realistic.
Thanks
Jon
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-cats.ucsc.edu
==============================Original Headers============================== 11, 21 -- From jmkrupp-at-cats.ucsc.edu Wed Nov 30 18:36:09 2005 11, 21 -- Received: from cats-mx1.ucsc.edu (cats-mx1.ucsc.edu [128.114.125.34]) 11, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB10a8eu012422 11, 21 -- for {microscopy-at-microscopy.com} ; Wed, 30 Nov 2005 18:36:08 -0600 11, 21 -- Received: from [128.114.25.151] (dhcp-25-151.ucsc.edu [128.114.25.151]) 11, 21 -- by cats-mx1.ucsc.edu (8.13.1/8.13.1) with SMTP id jB10YHAJ002955 11, 21 -- for {microscopy-at-microscopy.com} ; Wed, 30 Nov 2005 16:34:21 -0800 (PST) 11, 21 -- X-Sender: jmkrupp-at-cruzmail.ucsc.edu 11, 21 -- Message-Id: {v01550100bfb3f2222c9e-at-[128.114.25.135]} 11, 21 -- Mime-Version: 1.0 11, 21 -- Content-Type: text/plain; charset="us-ascii" 11, 21 -- Date: Wed, 30 Nov 2005 16:29:42 -0800 11, 21 -- To: microscopy-at-microscopy.com 11, 21 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp) 11, 21 -- Subject: comparing images 11, 21 -- X-UCSC-CATS-Information: This message was scanned by the ITS MailScanner 11, 21 -- X-UCSC-CATS-MailScanner: Found to be clean 11, 21 -- X-UCSC-CATS-MailScanner-SpamCheck: not spam, spamassassin (score=1.57, 11, 21 -- required 8, autolearn=disabled, MISSING_SUBJECT 1.57) 11, 21 -- X-UCSC-CATS-MailScanner-SpamScore: s 11, 21 -- X-UCSC-CATS-MailScanner-From: jmkrupp-at-cats.ucsc.edu ==============================End of - Headers==============================
==============================Original Headers============================== 27, 24 -- From Mike.Bode-at-soft-imaging.net Thu Dec 1 08:20:00 2005 27, 24 -- Received: from mail.soft-imaging.de (mail.soft-imaging.de [62.180.61.131]) 27, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB1EIwX9029278 27, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Dec 2005 08:19:18 -0600 27, 24 -- Received: from muenster.soft-imaging.net (muenster.soft-imaging.de [10.26.20.9]) 27, 24 -- by mail.soft-imaging.de (8.11.6/8.11.6/SuSE Linux 0.5) with ESMTP id jB1EIvP22915; 27, 24 -- Thu, 1 Dec 2005 15:18:57 +0100 27, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 27, 24 -- Content-class: urn:content-classes:message 27, 24 -- MIME-Version: 1.0 27, 24 -- Content-Type: text/plain; 27, 24 -- charset="iso-8859-1" 27, 24 -- Subject: RE: [Microscopy] comparing images 27, 24 -- Date: Thu, 1 Dec 2005 15:15:22 +0100 27, 24 -- Message-ID: {6D0150089E1EA046BD96C68C50608C23025CD2CB-at-ms-s-gws.soft-imaging.net} 27, 24 -- X-MS-Has-Attach: 27, 24 -- X-MS-TNEF-Correlator: 27, 24 -- Thread-Topic: [Microscopy] comparing images 27, 24 -- Thread-Index: AcX2E2LcjlZu1Kn/T/iQNGL86WOhHgAbR34A 27, 24 -- From: "Mike Bode" {Mike.Bode-at-soft-imaging.net} 27, 24 -- To: {Microscopy-at-microscopy.com} 27, 24 -- Cc: {jmkrupp-at-cats.ucsc.edu} 27, 24 -- Content-Transfer-Encoding: 8bit 27, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jB1EIwX9029278 ==============================End of - Headers==============================
Does anyone know if GFP fluorescence survives when formaldehyde-fixed tissues are permeabilized with Triton X-100? I need to double label a GFP expressing tissue. thanks, tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
We have a Hitichi H500 that we are decommissioning. My question(s) is how much oil is in the high tension tank and also does anyone still use one and would they be interested in parts? Please reply to me via list server or nicholls-at-post.queensu.ca
Rod Nicholls Histology/Electron Microscopy Technician Department of Anatomy and Cell Biology Queen's University Kingston, Ontario K7L 3N6 Phone: 613- 533 6000 x 78265
==============================Original Headers============================== 3, 15 -- From nicholls-at-post.queensu.ca Thu Dec 1 16:07:49 2005 3, 15 -- Received: from post.queensu.ca (post.QueensU.CA [130.15.126.6]) 3, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB1EWvVr029660 3, 15 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Dec 2005 08:33:13 -0600 3, 15 -- Received: from EMSuite.post.queensu.ca (U55.N149.QueensU.CA [130.15.149.55]) 3, 15 -- by post.queensu.ca (8.13.1/8.13.1) with ESMTP id jB1EWvYv012180 3, 15 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Dec 2005 09:32:57 -0500 (EST) 3, 15 -- Message-Id: {6.2.3.4.0.20051201092602.01d04010-at-post.queensu.ca} 3, 15 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 3, 15 -- Date: Thu, 01 Dec 2005 09:32:51 -0500 3, 15 -- To: Microscopy-at-microscopy.com 3, 15 -- From: Rod Nicholls {nicholls-at-post.queensu.ca} 3, 15 -- Subject: Hitachi H500 TEM decommisioning 3, 15 -- Mime-Version: 1.0 3, 15 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Dr Muss- Thanks for the paper and information about your uses of TA.
(The inserted PDF attachment you sent came to me through the list-server. How did that succeed? I will try here to to insert my PDF of the 1991 abstract (see below) and see if it passes to become available to other readers.)
I have been meaning for some time to try TA as a first-step section stain, as suggested by some papers of ~15 years ago (At the moment i can track down Haldar et al (J Clin Pathol, (1992), 45:633-635; use a MIX of TA + UrAc!) and Stirling (J.Histochem.Cytochem. (1993) 41:643-648.) Maybe I will finally do so, now that I know your procedure.
I'm interested that you keep it in the dark as a prepared solution. When we started using TA with GA in early 1979 (inspired by David Begg et al paper in J Cell Biol (1978) 79:846-852 we soon got the idea that we could not gain the benefits if improved contrast and better preservation if we stored TA as a prepared or stock solution. The color changed a bit after 1-2 days, became darker I think, and the structural preservation was not so nice. Ever since, we make it a habit to dissolve TA fresh, whether using it in a mix with GA or simply alone as primary fix, starting the fix no later than about 3 hours after dissolving the TA, same precaution whether in aqueous or cryo-substitution-acetone procedures. Perhaps keeping it in the dark might prolong its useful life in solution, but because we lost 2+ weeks of work back in 1979 before we learned our lesson, we chose after that not to explore the use of aged TA solutions.
The TA and TAMD dendromer paper you sent is interesting. We are at the moment more interested in low low MW mimics of TA like catechin, because even the best low MW TA seems unable to penetrate and carry stain mordanting properties to the interior of globular protein domains-- even if the UrAc or OsO4 is applied before the TA it does not attract the TA to the interior of myofilaments. The result is some degree of negative staining on the smallest scale; it appears to produce a dense nanoshell around actin monomers and myosin head globular domain in our most detailed EM tomography of thin sections from cryo-substituted insect flight muscle fibers (J Struct Biol. (2004) 147:268-282). (We have several more detailed and convincing density-contoured images than the published figure.) This shell remains more dens than any interior positive staining that may also occur, despite the very intense section staining produced by the permanganate -} Sato's Pb stain sequence we use. We suppose that TA is probably ALWAYS producing a surface coat on globular protein domains, regardless of whether it also may penetrate. Using catechin instead sometimes seemsed to make the staining more positive (by eye, not tomography) in some regions of sections from a muscle fiber so treated. TA may produce or allow more permeating and homogeneous staining of very slender 2-4 nm molecular strands like the lever-arm of the myosin heads, and even of much fatter structures, such as the M-region of thick filaments which often appear a glassy uniform gray despite the nano-negative staining of the adjacent thin filaments and the A-band.
All of this is to say why we are not immediately interested in trying the TA dendromers, and why we need to try TA (and probably also catehin) in a section staining procedure. Perhaps at the cut surface of a section it can penetrate internal regions of actin monomers and myosin heads and filament backbones that proved rather impenetrable when exposed to TA in liquid fixatives.
-mike reedy-
you must double-click on the apparently blank pdf just below to make it open in Acrobat and show its content.
} } } } Dear Dr. Reedy, } } I would like to thank you very much for your copy "service". } These abstracts I gladly shall integrate into my "library of EM-methods". } } I found TA (if "low molecular weight") very helpful in stabilizing } structures and demonstrating structure usually not "included" in stained } sections if processed the "normal" schedule (FA-GA,GA,OsO4,Dehydration). } } Also we (in a diagnostic EM-Lab) use this stuff within a pre-incubation } step (0.05-0.5% w/v hydrous solution, sonicated, filtered and stored in } plastic syringes in the dark, dispensed via a 0.25 or 0.42 ?m millipore } filternotch to grids, 5-15 min {usually} at room temperature), before } applying the classical UO2Ac-Lead-citrate staining sequence on to the } ultrathin sections. Then we will have (depending on application time, and } temperature, respectively) a very discrete to heavily e-dense staining of } e.g. collagen fibrils, glycogen (+/-"specifically") and some extracellular } matrix components usually not seen without that pretreatment. } } That effect is/will be increased by using a } para-phenylenediamine } (PPD) {step after osmication (i.e. OsO4 as usual, washing in buffer, EtOH } 50%, followed by an incubation of tissue samples in 1% PPD in 70%EtOH for } about 25-30 min-at-rtemp, washing several times unless solution is clear, } further dehydration-processing steps as usual). } Additionally, I found that such a treatment generally results in a better } stabilization of diseased tissue and therefore also better visualisation of } most tissue components at the ultrastructural level. } } I would like to send to you a paper as .pdf you perhaps might not have in } your library. } The article does not describe TA } used as a primary fixative in EM {, but, } instead, tells us about the mechanisms of effects exerted by TA-and } TA-mimicking dendrimers on to mucosal scaffolds for transplantations, as } evaluated also by TEM......interesting work..... } } } Best regards and wishes to you and yours, } } Wolfgang Muss } Salzburg/Austria } } ---------- } Von: Mike Reedy[SMTP:mike.reedy-at-cellbio.duke.edu] } Gesendet: Mittwoch, 30. November 2005 16:37 } An: W.Muss-at-salk.at } Betreff: Re: AW: [Microscopy] RE: Re: SEM imaging of bacteria } encapsulation } } { {Datei: 1991 BJ Abstracts -at- TAURAC, TAOS.pdf} } } } } } } Dear Dr Muss, } } Thank you for the kind holiday wishes. May yours be also wonderful! } Here is the PDF, with my hopes you find the method useful. } } -mike reedy- } } } } Good morning, } } } } dear Dr. Reedy, } } } } I greatly should appreciate receiving by e-mail the .pdf on the } } TAURAC-methods mentioned below (which by no means you will be able to } } transmit via the Listserver because any attachment will be blocked and } } deleted). } } } } Have a nice and calm "advent-" and a beautiful Christmas time, } } yours thankfully } } } } } } Wolfgang Muss PhD } } EM-Lab., PATHOLOGY SALK } } SALZBURG, Austria } } } } } } } } } } ---------- } } Von: mike.reedy-at-cellbio.duke.edu[SMTP:mike.reedy-at-cellbio.duke.edu] } } Antwort an: mike.reedy-at-cellbio.duke.edu } } Gesendet: Dienstag, 29. November 2005 23:22 } } An: W.Muss-at-salk.at } } Betreff: [Microscopy] Re: SEM imaging of bacteria encapsulation } } } } ------------------------------------------------------------------------ } } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
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Email: cadr-at-mba.ac.uk Name: catherine
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Title-Subject: [Filtered] Immunogold and marine larvae
Question: Hello,
I would like to perform an immunogold (TEM) on marine larvae. I am looking for some basic protocols.. .
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Email: kjl226-at-vt.edu Name: Kathy Lowe
Organization: Virginia Tec h, College of Vet. Med
Title-Subject: [Filtered] New TEM information
Question: I am interested in getting information from companies on their Transmission Electron Microscopes. We have a old Zeiss 10CA. I'm just interested in learning more about what's available. We deal mainly with biological samples with the exception of a few polymer samples.
Dear Don, The only comment that I might make is that silicon nitride and silicon are conductive, so they are suitable for SEM as is, whereas silicon oxide on silicon is an insulator and may cause charging problems in the SEM. Also, at low kVs the pure silicon will have a thin oxide layer that may also cause charging problems. Etching also tends to induce an oxide layer. Coating the sample will change its dimensions slightly. Regards, Mary Mager Electron Microscopist Department of Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA Tel: 604-822-5648 Fax: 604-822-3619 e-mail: mager-at-interchange.ubc.ca ----- Original Message ----- X-from: {donc-at-asmicro.com} To: {mager-at-interchange.ubc.ca} Sent: Wednesday, November 30, 2005 9:17 PM
Hi all, Does anyone have the Nikon DS - 5M camera on their light microscope? Is there a way to format the CF card with the DS Camera Control Unit DS-L1?
Beth
********************************************************************** Beth Richardson EM Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
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Does anyone know if GFP fluorescence survives when formaldehyde-fixed tissues are permeabilized with Triton X-100? I need to double label a GFP expressing tissue. thanks, tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
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Organization: Virginia Tec h, College of Vet. Med
Title-Subject: [Filtered] New TEM information
Question: I am interested in getting information from companies on their Transmission Electron Microscopes. We have a old Zeiss 10CA. I'm just interested in learning more about what's available. We deal mainly with biological samples with the exception of a few polymer samples.
Any comparison between images is complicated, as in most cases the acquisition conditions are not or can not be controlled so as to give you identical conditions. This is even more difficult in fluorescence images, as the flourescence can bleach over time, so not only the conditions, but also the timing must be right.
The first problem you can try to tackle by embedding some standards into the preparation. Perhaps some small fluorescent beads that you can then use to normalize the image. Instead of using the intensity itself, you would use the intensity ratio of sample/bead as a measure.
If your samples bleach, you can try to do severl things:
1) acquire a time series of each sample and measure the decay of the signal and extrapolate to time 0, or 2) acquire the image at precise times, for example 1 minute after turning on the illumination.
Good luck.
mike
Michael Bode, Ph.D. Soft Imaging System Corp. 12596 West Bayaud Avenue Suite 300 Lakewood, CO 80228 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- X-from: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu] Sent: Wednesday, November 30, 2005 6:06 PM To: Mike Bode
I have an unusual question, I will try to explain it as best I can.
A researcher has asked me to find out if it is reasonable to do anything like a quantitative comparison between fluorescence images of different samples.
She has thick sections, 20 um, on a confocal scope. She sees differences by eye between control and treatments, and she would like to quantify these differences in some way.
We have thought about setting up the confocal to record the brightest image, then without changing the settings, record an image of the other slides. The idea would be that she could do something like compare the brightness levels between them.
I don't know enough about other options or if this will even work to help her.
Does anyone do anything like this or is this not realistic.
Thanks
Jon
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-cats.ucsc.edu
==============================Original Headers============================== 11, 21 -- From jmkrupp-at-cats.ucsc.edu Wed Nov 30 18:36:09 2005 11, 21 -- Received: from cats-mx1.ucsc.edu (cats-mx1.ucsc.edu [128.114.125.34]) 11, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB10a8eu012422 11, 21 -- for {microscopy-at-microscopy.com} ; Wed, 30 Nov 2005 18:36:08 -0600 11, 21 -- Received: from [128.114.25.151] (dhcp-25-151.ucsc.edu [128.114.25.151]) 11, 21 -- by cats-mx1.ucsc.edu (8.13.1/8.13.1) with SMTP id jB10YHAJ002955 11, 21 -- for {microscopy-at-microscopy.com} ; Wed, 30 Nov 2005 16:34:21 -0800 (PST) 11, 21 -- X-Sender: jmkrupp-at-cruzmail.ucsc.edu 11, 21 -- Message-Id: {v01550100bfb3f2222c9e-at-[128.114.25.135]} 11, 21 -- Mime-Version: 1.0 11, 21 -- Content-Type: text/plain; charset="us-ascii" 11, 21 -- Date: Wed, 30 Nov 2005 16:29:42 -0800 11, 21 -- To: microscopy-at-microscopy.com 11, 21 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp) 11, 21 -- Subject: comparing images 11, 21 -- X-UCSC-CATS-Information: This message was scanned by the ITS MailScanner 11, 21 -- X-UCSC-CATS-MailScanner: Found to be clean 11, 21 -- X-UCSC-CATS-MailScanner-SpamCheck: not spam, spamassassin (score=1.57, 11, 21 -- required 8, autolearn=disabled, MISSING_SUBJECT 1.57) 11, 21 -- X-UCSC-CATS-MailScanner-SpamScore: s 11, 21 -- X-UCSC-CATS-MailScanner-From: jmkrupp-at-cats.ucsc.edu ==============================End of - Headers==============================
==============================Original Headers============================== 27, 24 -- From Mike.Bode-at-soft-imaging.net Thu Dec 1 16:28:01 2005 27, 24 -- Received: from mail.soft-imaging.de (mail.soft-imaging.de [62.180.61.131]) 27, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB1EIwX9029278 27, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Dec 2005 08:19:18 -0600 27, 24 -- Received: from muenster.soft-imaging.net (muenster.soft-imaging.de [10.26.20.9]) 27, 24 -- by mail.soft-imaging.de (8.11.6/8.11.6/SuSE Linux 0.5) with ESMTP id jB1EIvP22915; 27, 24 -- Thu, 1 Dec 2005 15:18:57 +0100 27, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 27, 24 -- Content-class: urn:content-classes:message 27, 24 -- MIME-Version: 1.0 27, 24 -- Content-Type: text/plain; 27, 24 -- charset="iso-8859-1" 27, 24 -- Subject: RE: [Microscopy] comparing images 27, 24 -- Date: Thu, 1 Dec 2005 15:15:22 +0100 27, 24 -- Message-ID: {6D0150089E1EA046BD96C68C50608C23025CD2CB-at-ms-s-gws.soft-imaging.net} 27, 24 -- X-MS-Has-Attach: 27, 24 -- X-MS-TNEF-Correlator: 27, 24 -- Thread-Topic: [Microscopy] comparing images 27, 24 -- Thread-Index: AcX2E2LcjlZu1Kn/T/iQNGL86WOhHgAbR34A 27, 24 -- From: "Mike Bode" {Mike.Bode-at-soft-imaging.net} 27, 24 -- To: {Microscopy-at-microscopy.com} 27, 24 -- Cc: {jmkrupp-at-cats.ucsc.edu} 27, 24 -- Content-Transfer-Encoding: 8bit 27, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jB1EIwX9029278 ==============================End of - Headers==============================
Dr Muss- Thanks for the paper and information about your uses of TA.
(The inserted PDF attachment you sent came to me through the list-server. How did that succeed? I will try here to to insert my PDF of the 1991 abstract (see below) and see if it passes to become available to other readers.)
I have been meaning for some time to try TA as a first-step section stain, as suggested by some papers of ~15 years ago (At the moment i can track down Haldar et al (J Clin Pathol, (1992), 45:633-635; use a MIX of TA + UrAc!) and Stirling (J.Histochem.Cytochem. (1993) 41:643-648.) Maybe I will finally do so, now that I know your procedure.
I'm interested that you keep it in the dark as a prepared solution. When we started using TA with GA in early 1979 (inspired by David Begg et al paper in J Cell Biol (1978) 79:846-852 we soon got the idea that we could not gain the benefits if improved contrast and better preservation if we stored TA as a prepared or stock solution. The color changed a bit after 1-2 days, became darker I think, and the structural preservation was not so nice. Ever since, we make it a habit to dissolve TA fresh, whether using it in a mix with GA or simply alone as primary fix, starting the fix no later than about 3 hours after dissolving the TA, same precaution whether in aqueous or cryo-substitution-acetone procedures. Perhaps keeping it in the dark might prolong its useful life in solution, but because we lost 2+ weeks of work back in 1979 before we learned our lesson, we chose after that not to explore the use of aged TA solutions.
The TA and TAMD dendromer paper you sent is interesting. We are at the moment more interested in low low MW mimics of TA like catechin, because even the best low MW TA seems unable to penetrate and carry stain mordanting properties to the interior of globular protein domains-- even if the UrAc or OsO4 is applied before the TA it does not attract the TA to the interior of myofilaments. The result is some degree of negative staining on the smallest scale; it appears to produce a dense nanoshell around actin monomers and myosin head globular domain in our most detailed EM tomography of thin sections from cryo-substituted insect flight muscle fibers (J Struct Biol. (2004) 147:268-282). (We have several more detailed and convincing density-contoured images than the published figure.) This shell remains more dens than any interior positive staining that may also occur, despite the very intense section staining produced by the permanganate -} Sato's Pb stain sequence we use. We suppose that TA is probably ALWAYS producing a surface coat on globular protein domains, regardless of whether it also may penetrate. Using catechin instead sometimes seemsed to make the staining more positive (by eye, not tomography) in some regions of sections from a muscle fiber so treated. TA may produce or allow more permeating and homogeneous staining of very slender 2-4 nm molecular strands like the lever-arm of the myosin heads, and even of much fatter structures, such as the M-region of thick filaments which often appear a glassy uniform gray despite the nano-negative staining of the adjacent thin filaments and the A-band.
All of this is to say why we are not immediately interested in trying the TA dendromers, and why we need to try TA (and probably also catehin) in a section staining procedure. Perhaps at the cut surface of a section it can penetrate internal regions of actin monomers and myosin heads and filament backbones that proved rather impenetrable when exposed to TA in liquid fixatives.
-mike reedy-
you must double-click on the apparently blank pdf just below to make it open in Acrobat and show its content.
} } } } Dear Dr. Reedy, } } I would like to thank you very much for your copy "service". } These abstracts I gladly shall integrate into my "library of EM-methods". } } I found TA (if "low molecular weight") very helpful in stabilizing } structures and demonstrating structure usually not "included" in stained } sections if processed the "normal" schedule (FA-GA,GA,OsO4,Dehydration). } } Also we (in a diagnostic EM-Lab) use this stuff within a pre-incubation } step (0.05-0.5% w/v hydrous solution, sonicated, filtered and stored in } plastic syringes in the dark, dispensed via a 0.25 or 0.42 ?m millipore } filternotch to grids, 5-15 min {usually} at room temperature), before } applying the classical UO2Ac-Lead-citrate staining sequence on to the } ultrathin sections. Then we will have (depending on application time, and } temperature, respectively) a very discrete to heavily e-dense staining of } e.g. collagen fibrils, glycogen (+/-"specifically") and some extracellular } matrix components usually not seen without that pretreatment. } } That effect is/will be increased by using a } para-phenylenediamine } (PPD) {step after osmication (i.e. OsO4 as usual, washing in buffer, EtOH } 50%, followed by an incubation of tissue samples in 1% PPD in 70%EtOH for } about 25-30 min-at-rtemp, washing several times unless solution is clear, } further dehydration-processing steps as usual). } Additionally, I found that such a treatment generally results in a better } stabilization of diseased tissue and therefore also better visualisation of } most tissue components at the ultrastructural level. } } I would like to send to you a paper as .pdf you perhaps might not have in } your library. } The article does not describe TA } used as a primary fixative in EM {, but, } instead, tells us about the mechanisms of effects exerted by TA-and } TA-mimicking dendrimers on to mucosal scaffolds for transplantations, as } evaluated also by TEM......interesting work..... } } } Best regards and wishes to you and yours, } } Wolfgang Muss } Salzburg/Austria } } ---------- } Von: Mike Reedy[SMTP:mike.reedy-at-cellbio.duke.edu] } Gesendet: Mittwoch, 30. November 2005 16:37 } An: W.Muss-at-salk.at } Betreff: Re: AW: [Microscopy] RE: Re: SEM imaging of bacteria } encapsulation } } { {Datei: 1991 BJ Abstracts -at- TAURAC, TAOS.pdf} } } } } } } Dear Dr Muss, } } Thank you for the kind holiday wishes. May yours be also wonderful! } Here is the PDF, with my hopes you find the method useful. } } -mike reedy- } } } } Good morning, } } } } dear Dr. Reedy, } } } } I greatly should appreciate receiving by e-mail the .pdf on the } } TAURAC-methods mentioned below (which by no means you will be able to } } transmit via the Listserver because any attachment will be blocked and } } deleted). } } } } Have a nice and calm "advent-" and a beautiful Christmas time, } } yours thankfully } } } } } } Wolfgang Muss PhD } } EM-Lab., PATHOLOGY SALK } } SALZBURG, Austria } } } } } } } } } } ---------- } } Von: mike.reedy-at-cellbio.duke.edu[SMTP:mike.reedy-at-cellbio.duke.edu] } } Antwort an: mike.reedy-at-cellbio.duke.edu } } Gesendet: Dienstag, 29. November 2005 23:22 } } An: W.Muss-at-salk.at } } Betreff: [Microscopy] Re: SEM imaging of bacteria encapsulation } } } } ------------------------------------------------------------------------ } } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
I would use an anti-GFP and do the labeling that way. There may be some GFP left after that treatment, but I would not count on it. GFP antibodies will work well. David
On Dec 1, 2005, at 3:23 PM, phillipst-at-missouri.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Does anyone know if GFP fluorescence survives when formaldehyde-fixed } tissues are permeabilized with Triton X-100? I need to double } label a GFP } expressing tissue. thanks, tom } } } } Thomas E. Phillips, PhD } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } } 573-882-4712 (office) } 573-882-0123 (fax) } PhillipsT-at-missouri.edu } } } } ==============================Original } Headers============================== } 7, 18 -- From PhillipsT-at-missouri.edu Thu Dec 1 16:21:09 2005 } 7, 18 -- Received: from um-exproto9.um.umsystem.edu (um- } exproto9.um.umsystem.edu [207.160.151.49]) } 7, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } jB1JEXqg013667 } 7, 18 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 1 Dec 2005 } 13:14:43 -0600 } 7, 18 -- Received: from um-exproto9.um.umsystem.edu } ([207.160.151.148]) by um-exproto9.um.umsystem.edu with Microsoft } SMTPSVC(6.0.3790.1830); } 7, 18 -- Thu, 1 Dec 2005 13:12:07 -0600 } 7, 18 -- Received: from phillips-dell.missouri.edu } ([128.206.81.132]) by um-exproto9.um.umsystem.edu over TLS secured } channel with Microsoft SMTPSVC(6.0.3790.1830); } 7, 18 -- Thu, 1 Dec 2005 13:12:06 -0600 } 7, 18 -- Message-Id: } {6.0.0.22.2.20051201131205.02007428-at-pop.missouri.edu} } 7, 18 -- X-Sender: phillipst-at-pop.missouri.edu } 7, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 6.0.0.22 } 7, 18 -- Date: Thu, 01 Dec 2005 13:13:45 -0600 } 7, 18 -- To: Microscopy-at-msa.microscopy.com } 7, 18 -- From: Tom Phillips {phillipst-at-missouri.edu} } 7, 18 -- Subject: GFP & Triton X-100 } 7, 18 -- Mime-Version: 1.0 } 7, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } 7, 18 -- X-OriginalArrivalTime: 01 Dec 2005 19:12:07.0052 (UTC) } FILETIME=[1F0A0CC0:01C5F6AB] } ==============================End of - } Headers============================== }
==============================Original Headers============================== 6, 18 -- From Elliott-at-Arizona.EDU Thu Dec 1 17:02:58 2005 6, 18 -- Received: from elliott-server.doctorelliott.us (doctorelliott.us [70.57.226.104]) 6, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB1N2wri016176 6, 18 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 1 Dec 2005 17:02:58 -0600 6, 18 -- Received: from [192.168.0.30] (unknown [192.168.0.30]) 6, 18 -- by elliott-server.doctorelliott.us (Postfix) with ESMTP id 221DD12186F 6, 18 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 1 Dec 2005 16:02:53 -0700 (MST) 6, 18 -- Mime-Version: 1.0 (Apple Message framework v734) 6, 18 -- In-Reply-To: {200512012223.jB1MNGGH014276-at-ns.microscopy.com} 6, 18 -- References: {200512012223.jB1MNGGH014276-at-ns.microscopy.com} 6, 18 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 18 -- Message-Id: {302A8F1D-B4C5-48BC-8CBE-7252FCDA2A61-at-Arizona.EDU} 6, 18 -- Content-Transfer-Encoding: 7bit 6, 18 -- From: David Elliott {Elliott-at-Arizona.EDU} 6, 18 -- Subject: Re: [Microscopy] GFP & Triton X-100 6, 18 -- Date: Thu, 1 Dec 2005 16:02:52 -0700 6, 18 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 6, 18 -- X-Mailer: Apple Mail (2.734) ==============================End of - Headers==============================
I'll be happy to help, but could you let me have some more info as to whether you need help on specimen preparation, or immunolabelling? There is a basic protocol on our website: http://www.aurion.nl via Technical Support} } Incubation Protocol that should work with any good quality gold conjugate.
Cheers
Jan Leunissen
Aurion - President Present Address: Costerweg 5 EM-Unit 6702 A Wageningen Otago School of Medicine The Netherlands Dunedin, New Zealand phone 31-317-497676 phone 64-3-4797109 fax 31-317-415955 fax 64-3-4797254 http://www.aurion.nl http://ocem.otago.ac.nz ------------------------------------------------------------------------ -------- "Light Microscopy? Is that for people on a diet? ". . . Eva Leunissen (12)
==============================Original Headers============================== 6, 24 -- From leunissen-at-aurion.nl Thu Dec 1 20:09:33 2005 6, 24 -- Received: from mta205-rme.xtra.co.nz (mta205-rme.xtra.co.nz [210.86.15.187]) 6, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB229VXm014120 6, 24 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 1 Dec 2005 20:09:32 -0600 6, 24 -- Received: from mta3-rme.xtra.co.nz ([210.86.15.192]) 6, 24 -- by mta205-rme.xtra.co.nz with ESMTP 6, 24 -- id {20051202020930.WTML1687.mta205-rme.xtra.co.nz-at-mta3-rme.xtra.co.nz} ; 6, 24 -- Fri, 2 Dec 2005 15:09:30 +1300 6, 24 -- Received: from [192.168.1.10] ([222.152.177.18]) by mta3-rme.xtra.co.nz 6, 24 -- with ESMTP 6, 24 -- id {20051202020930.JTDD14226.mta3-rme.xtra.co.nz-at-[192.168.1.10]} ; 6, 24 -- Fri, 2 Dec 2005 15:09:30 +1300 6, 24 -- In-Reply-To: {200512012211.jB1MBWf8005464-at-ns.microscopy.com} 6, 24 -- References: {200512012211.jB1MBWf8005464-at-ns.microscopy.com} 6, 24 -- Mime-Version: 1.0 (Apple Message framework v623) 6, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 24 -- Message-Id: {cefd22b6a02011e788510526aea5a0c5-at-aurion.nl} 6, 24 -- Content-Transfer-Encoding: 7bit 6, 24 -- Cc: cadr-at-mba.ac.uk 6, 24 -- From: Jan Leunissen {leunissen-at-aurion.nl} 6, 24 -- Subject: Re: [Microscopy] viaWWW: Immunogold and marine larvae 6, 24 -- Date: Fri, 2 Dec 2005 14:09:27 +1200 6, 24 -- To: "'microscopy-at-msa.microscopy.com'" {Microscopy-at-msa.microscopy.com} 6, 24 -- X-Mailer: Apple Mail (2.623) ==============================End of - Headers==============================
Last week we finished installation of a used JEOL 6100 SEM in our materials science department. But a scope only. Thus we need to purchase many items. We will do this one by one as necessary. Right now I am looking for a few essential equipments such as a sputter coater. Though we prefer to buy a used one, purchasing a new one can be an option.
So I would like to have your wise advice on purchasing a sputter coater. We need to do carbon coating and Au-Pd coating. Among mid to low price range, which brands have good reputations?
I used to use SEM(EBSD) and TEM while studying in U.S. Now I teach materials science in China. In this area, this is the first SEM installed. So many (colleges and local government) have interests in our SEM and services. Thank you very much in advance.
Elliot ++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Elliot K. Lee, Ph.D. Associate professor School of Materials, Mechanical & Automation Engineering Yanji city, Jilin Province CHINA (office) +86-433-291-2975 +++++++++++++++++++++++++++++++++++++++++++++++++++++++++
==============================Original Headers============================== 6, 20 -- From khlee-at-ybust.edu.cn Thu Dec 1 22:01:24 2005 6, 20 -- Received: from hope.yust.edu ([222.161.2.226]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB241KYB003551 6, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Dec 2005 22:01:22 -0600 6, 20 -- Message-Id: {200512020401.jB241KYB003551-at-ns.microscopy.com} 6, 20 -- Received: (qmail 27029 invoked by uid 89); 2 Dec 2005 04:13:41 -0000 6, 20 -- Received: from unknown (HELO DOWNTHERE) (127.0.0.1) 6, 20 -- by 0 with SMTP; 2 Dec 2005 04:13:41 -0000 6, 20 -- Received: from [222.161.2.237] by hope.yust.edu([222.161.2.226]); Fri, 02 Dec 2005 12:13:28 -0800 (CST) 6, 20 -- From: "Elliot Kyungho Lee" {khlee-at-ybust.edu.cn} 6, 20 -- To: {Microscopy-at-microscopy.com} 6, 20 -- Subject: SEM looking for a sputter coater 6, 20 -- Date: Fri, 2 Dec 2005 12:08:46 +0800 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="us-ascii" 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 6, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 6, 20 -- Thread-Index: AcX29hcBkBwP//E9TOyEtHkhKsv7Xw== ==============================End of - Headers==============================
first of all, thank you very much for your kind and informative reply.
I regret to say that for given reasons (see use of MSA Listserver) I did not respond to the MSA Listserver with an enclosed pdf, instead of, my reply only I directed to your personal e-mail-address. For all eventually interested in: The stated "TA and TAMD dendromer" paper entitled: Tannic acid mimicking dendrimers as small intestine submucosa stabilizing nanomordants by Vladimir Kasyanova, Jason Isenburg, Robert A. Draughn, Starr Hazard, Jason Hodde, Iveta Ozolanta, Modra Murovska, S. Bart Halkes, Ioannis Vrasidas, Rob M.J. Liskamp, Roland J. Pieters, Dan Simionescu, Roger R. Markwald, Vladimir Mironov*) has been published in Biomaterials, 27 (2006) 745-751 and there are copyrights attributed to Elsevier Publishers 2006, so I am not able to provide it on request.
So please, send your request to *) Corresponding author Tel.: +843792 7630; fax: +843 792 0664. E-mail address: mironovv-at-musc.edu (V. Mironov).
It is not the right time and place, perhaps, to go into further detail on all the very interesting facets of the information you gave on the use of TA in your lab....perhaps we can write offline about that. I would like to add here for all those interested the original reference for the ultrathin section pre-staining step (used before Uranyl-acetate-Lead citrate procedure) we use routinely since 1990 in our lab with very excellent results:
Rapid Contrasting of Extracellular Elements in Thin Sections Koert P. Dingernans and Marius A. van den Bergh Weerman Ultrastructural Pathology. 14/6:519-527, 1990 519-527
We use ultraclean glassware and stirrer, as well as triple distilled aqua for the solution of 0.05-05% TA (sonicating for 10 min or at least heating the solution, when stirring, up to approx. 45-50 degrees C) and store the TA-solution as described in a plastic syringe (25-30 ml) in the dark (several cautions at the time of filling as well of dispensing...i.e. filtering any way with a 0.25-0.45?m millipore filternotch) and in our experience the maximum storage time turns out to be approx. 3 months (storage at room temperature). Concerning the TA used (which in our experience is of utmost importance): since 1985 we use a batch of Tannic Acid of a defined Low Molecular Weight (approx. 1701, as we were told by the selling company MALLINCKRODT**), Order No. 1764, Tannic Acid Powder AR) and know that there are a lot of several } Tannic acid powders { out there which do not meet THAT low molecular weight......we think that this is a very important clue to good results. (Note: **) I do not have any interests in advertising that product or do have an affiliation with Mallinckrodt....perhaps other sources will have similar or even better product qualities).
I do have a .pdf version of this article which I scanned by myself for electronic storage in my "methods library" which is not printed quality but sufficient for reading the important steps necessary. Since the original publishing of that article is back now 16 years and as a private subscriber to } Ultrastructural Pathology { since 1988 I do hope not to hurt } copyrights {, if I respond to some colleagues requesting that .pdf for their (solely) personal use.
Best wishes and have all a beautiful "advent" and festive christmas time,
yours sincerely Wolfgang Muss SALZBURG, Austria (http://city.salzburg.com , German http://www2.salzburg.info// English)
---------- Von: Mike Reedy[SMTP:mike.reedy-at-cellbio.duke.edu] Gesendet: Donnerstag, 01. Dezember 2005 17:55 An: W.Muss-at-salk.at Cc: Microscopy Listserver Betreff: Re: AW: AW: [Microscopy] RE: Re: SEM imaging of bacteria encapsulation; tannic
Dr Muss- Thanks for the paper and information about your uses of TA.
(The inserted PDF attachment you sent came to me through the list-server. How did that succeed? I will try here to to insert my PDF of the 1991 abstract (see below) and see if it passes to become available to other readers.)
I have been meaning for some time to try TA as a first-step section stain, as suggested by some papers of ~15 years ago (At the moment i can track down Haldar et al (J Clin Pathol, (1992), 45:633-635; use a MIX of TA + UrAc!) and Stirling (J.Histochem.Cytochem. (1993) 41:643-648.) Maybe I will finally do so, now that I know your procedure.
I'm interested that you keep it in the dark as a prepared solution. When we started using TA with GA in early 1979 (inspired by David Begg et al paper in J Cell Biol (1978) 79:846-852 we soon got the idea that we could not gain the benefits if improved contrast and better preservation if we stored TA as a prepared or stock solution. The color changed a bit after 1-2 days, became darker I think, and the structural preservation was not so nice. Ever since, we make it a habit to dissolve TA fresh, whether using it in a mix with GA or simply alone as primary fix, starting the fix no later than about 3 hours after dissolving the TA, same precaution whether in aqueous or cryo-substitution-acetone procedures. Perhaps keeping it in the dark might prolong its useful life in solution, but because we lost 2+ weeks of work back in 1979 before we learned our lesson, we chose after that not to explore the use of aged TA solutions.
The TA and TAMD dendromer paper you sent is interesting. We are at the moment more interested in low low MW mimics of TA like catechin, because even the best low MW TA seems unable to penetrate and carry stain mordanting properties to the interior of globular protein domains-- even if the UrAc or OsO4 is applied before the TA it does not attract the TA to the interior of myofilaments. The result is some degree of negative staining on the smallest scale; it appears to produce a dense nanoshell around actin monomers and myosin head globular domain in our most detailed EM tomography of thin sections from cryo-substituted insect flight muscle fibers (J Struct Biol. (2004) 147:268-282). (We have several more detailed and convincing density-contoured images than the published figure.) This shell remains more dens than any interior positive staining that may also occur, despite the very intense section staining produced by the permanganate -} Sato's Pb stain sequence we use. We suppose that TA is probably ALWAYS producing a surface coat on globular protein domains, regardless of whether it also may penetrate. Using catechin instead sometimes seemsed to make the staining more positive (by eye, not tomography) in some regions of sections from a muscle fiber so treated. TA may produce or allow more permeating and homogeneous staining of very slender 2-4 nm molecular strands like the lever-arm of the myosin heads, and even of much fatter structures, such as the M-region of thick filaments which often appear a glassy uniform gray despite the nano-negative staining of the adjacent thin filaments and the A-band.
All of this is to say why we are not immediately interested in trying the TA dendromers, and why we need to try TA (and probably also catehin) in a section staining procedure. Perhaps at the cut surface of a section it can penetrate internal regions of actin monomers and myosin heads and filament backbones that proved rather impenetrable when exposed to TA in liquid fixatives.
-mike reedy-
you must double-click on the apparently blank pdf just below to make it open in Acrobat and show its content.
} } } } Dear Dr. Reedy, } } I would like to thank you very much for your copy "service". } These abstracts I gladly shall integrate into my "library of EM-methods". } } I found TA (if "low molecular weight") very helpful in stabilizing } structures and demonstrating structure usually not "included" in stained } sections if processed the "normal" schedule (FA-GA,GA,OsO4,Dehydration). } } Also we (in a diagnostic EM-Lab) use this stuff within a pre-incubation } step (0.05-0.5% w/v hydrous solution, sonicated, filtered and stored in } plastic syringes in the dark, dispensed via a 0.25 or 0.42 ?m millipore } filternotch to grids, 5-15 min {usually} at room temperature), before } applying the classical UO2Ac-Lead-citrate staining sequence on to the } ultrathin sections. Then we will have (depending on application time, and } temperature, respectively) a very discrete to heavily e-dense staining of } e.g. collagen fibrils, glycogen (+/-"specifically") and some extracellular } matrix components usually not seen without that pretreatment. } } That effect is/will be increased by using a } para-phenylenediamine } (PPD) {step after osmication (i.e. OsO4 as usual, washing in buffer, EtOH } 50%, followed by an incubation of tissue samples in 1% PPD in 70%EtOH for } about 25-30 min-at-rtemp, washing several times unless solution is clear, } further dehydration-processing steps as usual). } Additionally, I found that such a treatment generally results in a better } stabilization of diseased tissue and therefore also better visualisation of } most tissue components at the ultrastructural level. } } I would like to send to you a paper as .pdf you perhaps might not have in } your library. } The article does not describe TA } used as a primary fixative in EM {, but, } instead, tells us about the mechanisms of effects exerted by TA-and } TA-mimicking dendrimers on to mucosal scaffolds for transplantations, as } evaluated also by TEM......interesting work..... } } } Best regards and wishes to you and yours, } } Wolfgang Muss } Salzburg/Austria } } ---------- } Von: Mike Reedy[SMTP:mike.reedy-at-cellbio.duke.edu] } Gesendet: Mittwoch, 30. November 2005 16:37 } An: W.Muss-at-salk.at } Betreff: Re: AW: [Microscopy] RE: Re: SEM imaging of bacteria } encapsulation } } { {Datei: 1991 BJ Abstracts -at- TAURAC, TAOS.pdf} } } } } } } Dear Dr Muss, } } Thank you for the kind holiday wishes. May yours be also wonderful! } Here is the PDF, with my hopes you find the method useful. } } -mike reedy- } } } } Good morning, } } } } dear Dr. Reedy, } } } } I greatly should appreciate receiving by e-mail the .pdf on the } } TAURAC-methods mentioned below (which by no means you will be able to } } transmit via the Listserver because any attachment will be blocked and } } deleted). } } } } Have a nice and calm "advent-" and a beautiful Christmas time, } } yours thankfully } } } } } } Wolfgang Muss PhD } } EM-Lab., PATHOLOGY SALK } } SALZBURG, Austria } } } } } } } } } } ---------- } } Von: mike.reedy-at-cellbio.duke.edu[SMTP:mike.reedy-at-cellbio.duke.edu] } } Antwort an: mike.reedy-at-cellbio.duke.edu } } Gesendet: Dienstag, 29. November 2005 23:22 } } An: W.Muss-at-salk.at } } Betreff: [Microscopy] Re: SEM imaging of bacteria encapsulation } } } } ------------------------------------------------------------------------ } } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
For anyone interested in a calibration procedure, you can refer to ASTM E 766 (2003), "Standard Practice for Calibrating the Magnification of a Scanning Electron Microscope."
This procedure allows for the use of any suitable physical standard.
John Friel
==============================Original Headers============================== 4, 22 -- From jjf-at-pgt.com Fri Dec 2 06:33:39 2005 4, 22 -- Received: from smtp.enter.net (smtp.enter.net [216.193.128.24]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB2CXdIx023103 4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 2 Dec 2005 06:33:39 -0600 4, 22 -- Received: from localhost (mmail.enter.net [216.193.128.40]) 4, 22 -- by smtp.enter.net (Postfix) with ESMTP id 1417BCDE15 4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 2 Dec 2005 07:33:39 -0500 (EST) 4, 22 -- Received: from pgt.com (lvtnt-1-65.dialup.enter.net [216.193.144.75]) 4, 22 -- by smtp.enter.net (Postfix) with ESMTP id 5EC60CD8A5 4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 2 Dec 2005 07:33:38 -0500 (EST) 4, 22 -- Message-ID: {43903F46.5030709-at-pgt.com} 4, 22 -- Date: Fri, 02 Dec 2005 07:34:14 -0500 4, 22 -- From: John Friel {jjf-at-pgt.com} 4, 22 -- Reply-To: jjf-at-pgt.com 4, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.0.1) Gecko/20020823 Netscape/7.0 (nscd2) 4, 22 -- X-Accept-Language: en-us, en 4, 22 -- MIME-Version: 1.0 4, 22 -- To: microscopy-at-microscopy.com 4, 22 -- Subject: SEM Calibration Standard 4, 22 -- Content-Type: text/plain; charset=us-ascii; format=flowed 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- X-Virus-Checker-Version: Enter.Net Virus Scanner 1.1 ==============================End of - Headers==============================
X-from my limited experience with sputter coaters, I find that the Hummer brand is troublesome. The Denton brand seems to be favored among my colleagues.
Stu Smalinskas, P.E. Metallurgist SKF USA Plymouth, Michigan (734) 414-6862
--- khlee-at-ybust.edu.cn wrote:
} } Hi, } } Last week we finished installation of a used JEOL } 6100 SEM in our materials } science department. But a scope only. Thus we need } to purchase many items. } We will do this one by one as necessary. Right now I } am looking for a few } essential equipments such as a sputter coater. } Though we prefer to buy a } used one, purchasing a new one can be an option. } } So I would like to have your wise advice on } purchasing a sputter coater. We } need to do carbon coating and Au-Pd coating. Among } mid to low price range, } which brands have good reputations? } } I used to use SEM(EBSD) and TEM while studying in } U.S. Now I teach materials } science in China. In this area, this is the first } SEM installed. So many } (colleges and local government) have interests in } our SEM and services. } Thank you very much in advance. } } Elliot } ++++++++++++++++++++++++++++++++++++++++++++++++++++++++ } Elliot K. Lee, Ph.D. } Associate professor } School of Materials, Mechanical & Automation } Engineering } Yanji city, Jilin Province } CHINA } (office) +86-433-291-2975 } +++++++++++++++++++++++++++++++++++++++++++++++++++++++++ }
__________________________________ Start your day with Yahoo! - Make it your home page! http://www.yahoo.com/r/hs
==============================Original Headers============================== 7, 19 -- From smalinskas-at-yahoo.com Fri Dec 2 07:56:41 2005 7, 19 -- Received: from web34111.mail.mud.yahoo.com (web34111.mail.mud.yahoo.com [66.163.178.109]) 7, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jB2DueLX000627 7, 19 -- for {microscopy-at-sparc5.microscopy.com} ; Fri, 2 Dec 2005 07:56:40 -0600 7, 19 -- Received: (qmail 17592 invoked by uid 60001); 2 Dec 2005 13:56:40 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=Message-ID:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 7, 19 -- b=z0dGoNrq2+fkODDEe7S6DtF4PpTzm3dfoYVSoV6cs3Dx0eNhjhCjGJkGnzUgifkRbNEfjnj2X+urxOyJWHOC5WnHBL2vbmb53rWe4AH2b2sOXv2CQ674jCYLjxt5+ZOX+OqHYWkHVlTHzI7lPrwNIRfAk8GKLDYV5wfUwKeov38= ; 7, 19 -- Message-ID: {20051202135640.17590.qmail-at-web34111.mail.mud.yahoo.com} 7, 19 -- Received: from [141.151.33.213] by web34111.mail.mud.yahoo.com via HTTP; Fri, 02 Dec 2005 05:56:40 PST 7, 19 -- Date: Fri, 2 Dec 2005 05:56:40 -0800 (PST) 7, 19 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 7, 19 -- Subject: Re: [Microscopy] SEM looking for a sputter coater 7, 19 -- To: khlee-at-ybust.edu.cn, microscopy-at-ns.microscopy.com 7, 19 -- In-Reply-To: {200512020402.jB242xdM006554-at-ns.microscopy.com} 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=iso-8859-1 7, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both m_mo_shad-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: m_mo_shad-at-yahoo.com Name: Mary Shad
Title-Subject: [Filtered] casein micelles
Question: why should we use SEM and TEM both togather specially for studing casein micelles in milk?
what is the best way for determination the size of casein micelles by using elctron microscopy?
And what is the best way for sample preparation of casein micelles that we have a little change in casein micelle size?
I am doing some research with Tungsten Oxide. I got WO2.9 phase which is tetragonal phase with space group of P4/nmm (from XRD). I wonder if anybody have the atomic positions and Waykoff notations for this phase.
Thank You
Jafar
Rutgers University
==============================Original Headers============================== 7, 26 -- From jafarhan-at-rci.rutgers.edu Fri Dec 2 09:16:15 2005 7, 26 -- Received: from annwn1.rutgers.edu (nbcs-av.rutgers.edu [128.6.72.254]) 7, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB2FGFQl019697 7, 26 -- for {microscopy-at-microscopy.com} ; Fri, 2 Dec 2005 09:16:15 -0600 7, 26 -- Received: from localhost (localhost.rutgers.edu [127.0.0.1]) 7, 26 -- by annwn1.rutgers.edu (Postfix) with ESMTP id DA8A744E86 7, 26 -- for {microscopy-at-microscopy.com} ; Fri, 2 Dec 2005 10:15:59 -0500 (EST) 7, 26 -- Received: from annwn1.rutgers.edu ([127.0.0.1]) 7, 26 -- by localhost (annwn1.rutgers.edu [127.0.0.1]) (amavisd-new, port 10024) 7, 26 -- with ESMTP id 24046-05 for {microscopy-at-microscopy.com} ; 7, 26 -- Fri, 2 Dec 2005 10:15:59 -0500 (EST) 7, 26 -- Received: from rci.rutgers.edu (gehenna.rutgers.edu [128.6.72.72]) 7, 26 -- by annwn1.rutgers.edu (Postfix) with ESMTP id ADAF34423A 7, 26 -- for {microscopy-at-microscopy.com} ; Fri, 2 Dec 2005 10:15:59 -0500 (EST) 7, 26 -- Received: from galt.rci.rutgers.edu (jafarhan2.engr.rutgers.edu [128.6.22.35]) 7, 26 -- by rci.rutgers.edu (Postfix) with ESMTP id 5D54E12F6 7, 26 -- for {microscopy-at-microscopy.com} ; Fri, 2 Dec 2005 10:16:14 -0500 (EST) 7, 26 -- Message-Id: {6.0.1.1.0.20051129205315.01999ff0-at-rci.rutgers.edu} 7, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 6.0.1.1 7, 26 -- Date: Tue, 29 Nov 2005 21:24:01 -0500 7, 26 -- To: microscopy-at-microscopy.com 7, 26 -- From: Jafar Al-Sharab {jafarhan-at-rci.rutgers.edu} 7, 26 -- Subject: Atomic positions for WO2.9 7, 26 -- Mime-Version: 1.0 7, 26 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 26 -- X-Virus-Scanned: Virus Scanned by NBCS ==============================End of - Headers==============================
Does anyone have experience in contrasting LR white embedded thin sections with uranyl acetate and lead citrate?
I am new to using LR white, and tried halving the "normal" incubation times in uranyl acetate and lead citrate, but the sections are unusable- much too dark and full of stain deposits.
Can anybody with experience with LR White suggest a staining protocol?
thank you.
Dr. Gerd Leitinger
Institut für Zellbiologie, Histologie und Embryologie Medizinische Universität Graz Harrachgasse 21 A-8010 Graz Austria
Gerd, I contrast LR White thin sections with uranyl acetate (3% aqueous) for 5 minutes followed by 3 minutes in Pb citrate (Venable & Coggeshall). Wash thoroughly with water after the UA and with 0.01N NaOH followed by water after the lead. I have found this to give good contrast, and I can still see the gold label easily. Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175 http://www.cornellcelldevbiology.org
==============================Original Headers============================== 1, 21 -- From lcgould-at-med.cornell.edu Fri Dec 2 11:02:06 2005 1, 21 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45]) 1, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB2H2612006585 1, 21 -- for {microscopy-at-microscopy.com} ; Fri, 2 Dec 2005 11:02:06 -0600 1, 21 -- Received: from mpx2.med.cornell.edu (pc113142-10.med.cornell.edu [140.251.11.119]) 1, 21 -- by smtp-gw2.med.cornell.edu (Switch-3.1.7/Switch-3.1.7) with ESMTP id jB2H212c015313 1, 21 -- for {microscopy-at-microscopy.com} ; Fri, 2 Dec 2005 12:02:03 -0500 (EST) 1, 21 -- Received: from [140.251.145.131] by mpx2.med.cornell.edu 1, 21 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 1, 21 -- with ESMTP id {0IQV00HZZRBCDIC0-at-mpx2.med.cornell.edu} for 1, 21 -- microscopy-at-microscopy.com; Fri, 02 Dec 2005 12:02:01 -0500 (EST) 1, 21 -- Date: Fri, 02 Dec 2005 12:01:12 -0500 1, 21 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 1, 21 -- Subject: Re: [Microscopy] EM: LR White contrast 1, 21 -- In-reply-to: {200512021555.jB2FtsVY030538-at-ns.microscopy.com} 1, 21 -- To: gerd.leitinger-at-meduni-graz.at, microscopy-at-microscopy.com 1, 21 -- Message-id: {p06200705bfb62d3f0b94-at-[140.251.145.131]} 1, 21 -- MIME-version: 1.0 1, 21 -- Content-type: text/plain; charset=us-ascii; format=flowed 1, 21 -- References: {200512021555.jB2FtsVY030538-at-ns.microscopy.com} 1, 21 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.1.0.0, Antispam-Data: 2005.12.2.16 ==============================End of - Headers==============================
We are trying to decide whether to buy new or to have resharpened a couple of Diatome diamond knives. Has anyone had any experience with the Diatome resharpened knives? Are they as good as new? What was the turn-around time for resharpening?
Thanks for your comments,
Dotty Sorenson
Dorothy Sorenson Microscopy and Image-analysis Laboratory Department of Cell and Developmental Biology University Of Michigan Medical School 4643 Medical Science Building II 1301 Catherine Ann Arbor, MI 48109-0616 (734)763-1170 FAX (734)763-1166
==============================Original Headers============================== 6, 16 -- From dsoren-at-umich.edu Fri Dec 2 15:08:54 2005 6, 16 -- Received: from withoutevidence.mr.itd.umich.edu (withoutevidence.mr.itd.umich.edu [141.211.93.147]) 6, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB2L8sfr019545 6, 16 -- for {microscopy-at-msa.microscopy.com} ; Fri, 2 Dec 2005 15:08:54 -0600 6, 16 -- Received: from [141.214.169.65] (host-65.subnet-169.med.umich.edu [141.214.169.65]) 6, 16 -- by withoutevidence.mr.itd.umich.edu (smtp) with ESMTP id jB2L8rHf012732 6, 16 -- for {microscopy-at-msa.microscopy.com} ; Fri, 2 Dec 2005 16:08:53 -0500 6, 16 -- Mime-Version: 1.0 (Apple Message framework v746.2) 6, 16 -- Content-Transfer-Encoding: 7bit 6, 16 -- Message-Id: {7C7A493A-E001-4BD9-A314-FA08A858DFFA-at-umich.edu} 6, 16 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 16 -- To: microscopy-at-msa.microscopy.com 6, 16 -- From: Dorothy Sorenson {dsoren-at-umich.edu} 6, 16 -- Subject: diamond knives resharpening 6, 16 -- Date: Fri, 2 Dec 2005 16:07:25 -0500 6, 16 -- X-Mailer: Apple Mail (2.746.2) ==============================End of - Headers==============================
My understanding is that unless you specifically request re-sharpening, what you get is a new knife that is about the same size as what you started with. New diamond and new boat. It's well worth the money. David
On Dec 2, 2005, at 2:13 PM, dsoren-at-umich.edu wrote:
} } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Hello listers, } } We are trying to decide whether to buy new or to have resharpened a } couple of Diatome diamond knives. Has anyone had any experience with } the Diatome resharpened knives? Are they as good as new? What was } the turn-around time for resharpening? } } Thanks for your comments, } } Dotty Sorenson } } Dorothy Sorenson } Microscopy and Image-analysis Laboratory } Department of Cell and Developmental Biology } University Of Michigan Medical School } 4643 Medical Science Building II } 1301 Catherine } Ann Arbor, MI 48109-0616 } (734)763-1170 } FAX (734)763-1166 } } } ==============================Original } Headers============================== } 6, 16 -- From dsoren-at-umich.edu Fri Dec 2 15:08:54 2005 } 6, 16 -- Received: from withoutevidence.mr.itd.umich.edu } (withoutevidence.mr.itd.umich.edu [141.211.93.147]) } 6, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } jB2L8sfr019545 } 6, 16 -- for {microscopy-at-msa.microscopy.com} ; Fri, 2 Dec 2005 } 15:08:54 -0600 } 6, 16 -- Received: from [141.214.169.65] } (host-65.subnet-169.med.umich.edu [141.214.169.65]) } 6, 16 -- by withoutevidence.mr.itd.umich.edu (smtp) with ESMTP id } jB2L8rHf012732 } 6, 16 -- for {microscopy-at-msa.microscopy.com} ; Fri, 2 Dec 2005 } 16:08:53 -0500 } 6, 16 -- Mime-Version: 1.0 (Apple Message framework v746.2) } 6, 16 -- Content-Transfer-Encoding: 7bit } 6, 16 -- Message-Id: {7C7A493A-E001-4BD9-A314-FA08A858DFFA-at-umich.edu} } 6, 16 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; } format=flowed } 6, 16 -- To: microscopy-at-msa.microscopy.com } 6, 16 -- From: Dorothy Sorenson {dsoren-at-umich.edu} } 6, 16 -- Subject: diamond knives resharpening } 6, 16 -- Date: Fri, 2 Dec 2005 16:07:25 -0500 } 6, 16 -- X-Mailer: Apple Mail (2.746.2) } ==============================End of - } Headers==============================
==============================Original Headers============================== 5, 22 -- From Elliott-at-Arizona.edu Fri Dec 2 15:25:01 2005 5, 22 -- Received: from smtpgate.email.arizona.edu (deagol.email.Arizona.EDU [128.196.133.142]) 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB2LP0MC028671 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 2 Dec 2005 15:25:00 -0600 5, 22 -- Received: from localhost (faramir.email.arizona.edu [10.0.0.218]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 40CE9BDD82E 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 2 Dec 2005 14:25:00 -0700 (MST) 5, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id CAD38BD92A9 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 2 Dec 2005 14:24:58 -0700 (MST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v623) 5, 22 -- In-Reply-To: {200512022113.jB2LDoFu025369-at-ns.microscopy.com} 5, 22 -- References: {200512022113.jB2LDoFu025369-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {c097aa3fba018cdfdaa73f4e525951ad-at-Arizona.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: David Elliott {Elliott-at-Arizona.edu} 5, 22 -- Subject: Re: [Microscopy] diamond knives resharpening 5, 22 -- Date: Fri, 2 Dec 2005 14:24:58 -0700 5, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 22 -- X-Mailer: Apple Mail (2.623) 5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Gerd, I have been using LR white embedded animal tissue for my research. After having immuno labelled the grids, I stain them with 5% UA for 10 mins follwed by a 1 min rinse in distilled water. I use few pellets of NaOH in a covered petridish containing lead citrate drops and incubate the grids in lead citrate for 10 mins. Thereafter a quick rinse for 1 min in distilled water and view the grids after they have been airdried for about 5 mins. regards, Vinod Grad Student, Dept. Of Biology New Mexico State University,
On 12/2/05, lcgould-at-med.cornell.edu {lcgould-at-med.cornell.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Gerd, } I contrast LR White thin sections with uranyl acetate (3% aqueous) } for 5 minutes followed by 3 minutes in Pb citrate (Venable & } Coggeshall). Wash thoroughly with water after the UA and with 0.01N } NaOH followed by water after the lead. } I have found this to give good contrast, and I can still see the gold } label easily. } Lee } -- } Leona Cohen-Gould, M.S. } Sr. Staff Associate } Director, Electron Microscopy & Histology Core Facility } Manager, Optical Microscopy Core Facility } Joan & Sanford I. Weill Medical College } of Cornell University } voice (212)746-6146 } fax (212)746-8175 } http://www.cornellcelldevbiology.org } } ==============================Original Headers============================== } 1, 21 -- From lcgould-at-med.cornell.edu Fri Dec 2 11:02:06 2005 } 1, 21 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45]) } 1, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB2H2612006585 } 1, 21 -- for {microscopy-at-microscopy.com} ; Fri, 2 Dec 2005 11:02:06 -0600 } 1, 21 -- Received: from mpx2.med.cornell.edu (pc113142-10.med.cornell.edu [140.251.11.119]) } 1, 21 -- by smtp-gw2.med.cornell.edu (Switch-3.1.7/Switch-3.1.7) with ESMTP id jB2H212c015313 } 1, 21 -- for {microscopy-at-microscopy.com} ; Fri, 2 Dec 2005 12:02:03 -0500 (EST) } 1, 21 -- Received: from [140.251.145.131] by mpx2.med.cornell.edu } 1, 21 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) } 1, 21 -- with ESMTP id {0IQV00HZZRBCDIC0-at-mpx2.med.cornell.edu} for } 1, 21 -- microscopy-at-microscopy.com; Fri, 02 Dec 2005 12:02:01 -0500 (EST) } 1, 21 -- Date: Fri, 02 Dec 2005 12:01:12 -0500 } 1, 21 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} } 1, 21 -- Subject: Re: [Microscopy] EM: LR White contrast } 1, 21 -- In-reply-to: {200512021555.jB2FtsVY030538-at-ns.microscopy.com} } 1, 21 -- To: gerd.leitinger-at-meduni-graz.at, microscopy-at-microscopy.com } 1, 21 -- Message-id: {p06200705bfb62d3f0b94-at-[140.251.145.131]} } 1, 21 -- MIME-version: 1.0 } 1, 21 -- Content-type: text/plain; charset=us-ascii; format=flowed } 1, 21 -- References: {200512021555.jB2FtsVY030538-at-ns.microscopy.com} } 1, 21 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.1.0.0, Antispam-Data: 2005.12.2.16 } ==============================End of - Headers============================== }
==============================Original Headers============================== 3, 25 -- From nairvinods-at-gmail.com Fri Dec 2 17:12:15 2005 3, 25 -- Received: from wproxy.gmail.com (wproxy.gmail.com [64.233.184.198]) 3, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB2NCE8T010043 3, 25 -- for {microscopy-at-microscopy.com} ; Fri, 2 Dec 2005 17:12:14 -0600 3, 25 -- Received: by wproxy.gmail.com with SMTP id i28so218478wra 3, 25 -- for {microscopy-at-microscopy.com} ; Fri, 02 Dec 2005 15:12:14 -0800 (PST) 3, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 3, 25 -- s=beta; d=gmail.com; 3, 25 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 3, 25 -- b=mxlYlo5OEYJ7jeage4nYaxIxnxX+lPqA1yl3HTww/gNxwWl8KWCdh1CEAtvjyXegUeYkw07q7Mrb5lCpB4OCuAXD+0mUdC8P4wZvOrWzCYxbpTsTfXOl1pinzUGxEXTIchoLQ7RCL5E5BWtyWJaz6qIhbSUNQtayCGyAnfe6ZD0= 3, 25 -- Received: by 10.64.196.9 with SMTP id t9mr1864749qbf; 3, 25 -- Fri, 02 Dec 2005 15:12:14 -0800 (PST) 3, 25 -- Received: by 10.64.199.16 with HTTP; Fri, 2 Dec 2005 15:12:13 -0800 (PST) 3, 25 -- Message-ID: {ea42a3900512021512x5732a39frd8cca7a62eb2f9fa-at-mail.gmail.com} 3, 25 -- Date: Fri, 2 Dec 2005 16:12:13 -0700 3, 25 -- From: Vinod Nair {nairvinods-at-gmail.com} 3, 25 -- To: microscopy-at-microscopy.com 3, 25 -- Subject: Re: [Microscopy] Re: EM: LR White contrast 3, 25 -- In-Reply-To: {200512021706.jB2H6v97014428-at-ns.microscopy.com} 3, 25 -- MIME-Version: 1.0 3, 25 -- Content-Type: text/plain; charset=UTF-8 3, 25 -- Content-Disposition: inline 3, 25 -- References: {200512021706.jB2H6v97014428-at-ns.microscopy.com} 3, 25 -- Content-Transfer-Encoding: 8bit 3, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id jB2NCE8T010043 ==============================End of - Headers==============================
FEI Company (www.feicompany.com) is a leading supplier of "tools for Nanotechnology". We develop and manufacture tools that enable research, development and manufacture of Nanoscale features by helping our customers understand their three-dimensional structures. We serve the semiconductor, data storage, structural biology and industrial markets, where decreasing feature sizes drive the need for our structural process technology solutions. Our solutions are based on a combination of patented and proprietary technologies that provide industry-leading capabilities to view, measure, analyze, and modify physical structures at atomic, or nanometer scale resolutions. Simply put, FEI helps the world of nanotechnology develop products faster, control manufacturing processes better, and understand the structures of complex substances.
Job Location: Oregon, USA
Job Responsibilities:
The Senior Applications Engineer in the Dual Beam Group is responsible for providing technical expertise in the support of FEI's North American sales efforts. Primary responsibilities include the following:
* Performing high quality dual beam product demonstrations, including the development of new techniques and applications for FEI partners and customers. * Cultivating positive customer relationships and acting as a high level customer interface for specific instrument/applications issues, acting as a technical expert, explaining complex technology details, giving in depth presentations, and assisting in technique development. * Supporting and training customers after the sale has been made and the system is signed off. Supporting Service Engineers on instrument sign offs and specific application techniques. * Providing continuous feedback to the product groups on system performance, features, and problems while collaborating with marketing/development groups on future product developments.
Position Requirements -
This position is ideal for an experienced technologist wanting to be involved with a dynamic team and exposed to a constant variety of customer application areas. The successful candidate will possess the following combination of education and experience:
* At a minimum, a BS degree in materials, physics or chemical engineering. Higher degree and advanced knowledge about electrical engineering and/or Semiconductor Fabrication is greatly preferred. * 3 or more years experience in a relevant commercial/research establishment with recent experience utilizing Focused Ion Beam (FIB) and Scanning Electron Microscopy (SEM) technology in sample preparation, preferably across a variety of application areas, i.e., nanoelectronics, materials, thin films, etc. Additional experience in the areas of SEM/EDS, STEM and TEM is greatly preferred. * Working knowledge and confidence with PC platforms, scripting, Windows 2000 and PowerPoint desirable. * Ability to write technical papers, technical application reports and training manuals * Excellent, enthusiastic, clear communication skills with a diverse audience in a commercial sales focused environment is critical to the success of this position. * Ability to travel both domestically as well as internationally and possession of a valid passport.
Please send your resume to: Trish Rice, trice-at-feico.com
==============================Original Headers============================== 17, 20 -- From lgiannuzzi-at-adelphia.net Fri Dec 2 17:20:32 2005 17, 20 -- Received: from mta9.adelphia.net (mta9.adelphia.net [68.168.78.199]) 17, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB2NKWmn019007 17, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 2 Dec 2005 17:20:32 -0600 17, 20 -- Received: from Lu ([68.170.226.44]) by mta9.adelphia.net 17, 20 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 17, 20 -- id {20051202232032.HRRS19342.mta9.adelphia.net-at-Lu} 17, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 2 Dec 2005 18:20:32 -0500 17, 20 -- From: "Lucille A Giannuzzi" {lgiannuzzi-at-adelphia.net} 17, 20 -- To: {Microscopy-at-microscopy.com} 17, 20 -- Subject: 17, 20 -- Date: Fri, 2 Dec 2005 18:20:30 -0500 17, 20 -- MIME-Version: 1.0 17, 20 -- Content-Type: text/plain; 17, 20 -- charset="us-ascii" 17, 20 -- Content-Transfer-Encoding: 7bit 17, 20 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353 17, 20 -- Thread-Index: AcX3lvxwfbneGM5XQo2hQ+XkZG8UHw== 17, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 17, 20 -- Message-Id: {20051202232032.HRRS19342.mta9.adelphia.net-at-Lu} ==============================End of - Headers==============================
IMO, the Quantomix units are very nice but horribly expensive. If SPI has an equivalent but lower cost alternative, this is good.
The basic idea/concept is quite novel. I saw this at M&M 2005 and liked it. But the cost was a big detractor.
gary g.
At 10:14 AM 11/27/2005, you wrote:
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==============================Original Headers============================== 11, 26 -- From gary-at-gaugler.com Fri Dec 2 22:17:15 2005 11, 26 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jB34HFkB031275 11, 26 -- for {microscopy-at-microscopy.com} ; Fri, 2 Dec 2005 22:17:15 -0600 11, 26 -- Received: (qmail 25548 invoked from network); 2 Dec 2005 20:16:01 -0800 11, 26 -- Received: by simscan 1.1.0 ppid: 25541, pid: 25542, t: 3.7297s 11, 26 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1142 spam: 3.0.3 11, 26 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 26 -- by qsmtp2 with SMTP; 2 Dec 2005 20:15:57 -0800 11, 26 -- Message-Id: {6.2.3.4.2.20051202201427.02959e00-at-mail.calweb.com} 11, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 11, 26 -- Date: Fri, 02 Dec 2005 20:17:12 -0800 11, 26 -- To: cgarber-at-2spi.com 11, 26 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 26 -- Subject: =?iso-8859-1?Q?Re:_[Microscopy]_Quantomix=AE_sample_holders?= 11, 26 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 26 -- In-Reply-To: {200511271814.jARIE0Y0017468-at-ns.microscopy.com} 11, 26 -- References: {200511271814.jARIE0Y0017468-at-ns.microscopy.com} 11, 26 -- Mime-Version: 1.0 11, 26 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 11, 26 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp2.surewest.net 11, 26 -- X-Spam-Level: 11, 26 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00, 11, 26 -- MIME_QP_LONG_LINE autolearn=ham version=3.0.3 11, 26 -- Content-Transfer-Encoding: 8bit 11, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jB34HFkB031275 ==============================End of - Headers==============================
I echo this. I replaced two sequential Hummers with a new Denton Desk IV TSC. For ultra fine coating, the TSC turbo is essential. To absolutely get rid of hydrocarbon contamination, I had the standard oil diaphram pump replaced with an Edwards XDS5 dry scroll pump. It takes a little longer to coat from start to finish but the results are stunning.
The SEM is totally dry pumped.
Having said this, you cannot expect that your very special specimen will always be hydrocarbon-free. Being exposed to atmosphere for not all that long of time will compromise it. So get a dessicator or vacuum container to hold the specimens after coating and use. You WILL see a difference if not. The weak link in the chain is the oil mech pump for the dessicator unit. This can be solved by using an anti-backstreaming trap, dessicator chamber and other measures.
The key is keep oil and atmosphere away from the specimen. Otherwise use 1-3KV and deal with it. Wide field first, high mag last.
gary g.
At 05:58 AM 12/2/2005, you wrote:
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==============================Original Headers============================== 15, 24 -- From gary-at-gaugler.com Sat Dec 3 00:09:25 2005 15, 24 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 15, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jB369Pw8009101 15, 24 -- for {microscopy-at-microscopy.com} ; Sat, 3 Dec 2005 00:09:25 -0600 15, 24 -- Received: (qmail 7959 invoked from network); 2 Dec 2005 22:08:35 -0800 15, 24 -- Received: by simscan 1.1.0 ppid: 7948, pid: 7949, t: 3.7150s 15, 24 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1200 spam: 3.0.3 15, 24 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 15, 24 -- by qsmtp3 with SMTP; 2 Dec 2005 22:08:31 -0800 15, 24 -- Message-Id: {6.2.3.4.2.20051202215849.028ea4d0-at-mail.calweb.com} 15, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 15, 24 -- Date: Fri, 02 Dec 2005 22:09:23 -0800 15, 24 -- To: smalinskas-at-yahoo.com 15, 24 -- From: Gary Gaugler {gary-at-gaugler.com} 15, 24 -- Subject: Re: [Microscopy] Re: SEM looking for a sputter coater 15, 24 -- Cc: MSA listserver {microscopy-at-microscopy.com} 15, 24 -- In-Reply-To: {200512021358.jB2Dwj7W003927-at-ns.microscopy.com} 15, 24 -- References: {200512021358.jB2Dwj7W003927-at-ns.microscopy.com} 15, 24 -- Mime-Version: 1.0 15, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 15, 24 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp3.surewest.net 15, 24 -- X-Spam-Level: 15, 24 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 15, 24 -- version=3.0.3 ==============================End of - Headers==============================
Asylum Research will present "How to Choose an Atomic Force Microscope for Biological Research", tutorial at the American Society for Cell Biology Conference (ASCB).
Place: Moscone Convention Center, San Francisco
Additional information can be found at http://www.asylumresearch.com/ News/Events.shtml.
Regards, Terry Mehr Asylum Research www.AsylumResearch.com
==============================Original Headers============================== 5, 13 -- From terry-at-AsylumResearch.com Mon Dec 5 12:41:27 2005 5, 13 -- Received: from exchange.AsylumResearch.com (exchange.asylumresearch.com [207.154.79.129]) 5, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB5IfQwB010461 5, 13 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Dec 2005 12:41:27 -0600 5, 13 -- Mime-Version: 1.0 (Apple Message framework v746.2) 5, 13 -- Content-Transfer-Encoding: 7bit 5, 13 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 13 -- To: Microscopy-at-microscopy.com 5, 13 -- From: Terry Mehr {terry-at-AsylumResearch.com} 5, 13 -- Subject: Atomic Force Microscopy Tutorial at ASCB 5, 13 -- Date: Mon, 5 Dec 2005 10:41:24 -0800 5, 13 -- X-Mailer: Apple Mail (2.746.2) 5, 13 -- Message-ID: {1EjLHB-00030Z-BE-at-exchange.AsylumResearch.com} ==============================End of - Headers==============================
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Many thanks to all those who responded to my question about new vs resharpened diamond knives. The overwhelming consensus is that resharpened are as good as new and that the turn-around time for resharpening is around 4 weeks.
Dotty
Dorothy Sorenson Microscopy and Image-analysis Laboratory Department of Cell and Developmental Biology University Of Michigan Medical School 4643 Medical Science Building II 1301 Catherine Ann Arbor, MI 48109-0616 (734)763-1170 FAX (734)763-1166
==============================Original Headers============================== 4, 16 -- From dsoren-at-umich.edu Mon Dec 5 14:34:40 2005 4, 16 -- Received: from playinggod.mr.itd.umich.edu (playinggod.mr.itd.umich.edu [141.211.14.79]) 4, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB5KYeK1021559 4, 16 -- for {microscopy-at-microscopy.com} ; Mon, 5 Dec 2005 14:34:40 -0600 4, 16 -- Received: from [141.214.169.65] (host-65.subnet-169.med.umich.edu [141.214.169.65]) 4, 16 -- by playinggod.mr.itd.umich.edu (smtp) with ESMTP id jB5KYenY012122 4, 16 -- for {microscopy-at-microscopy.com} ; Mon, 5 Dec 2005 15:34:40 -0500 4, 16 -- Mime-Version: 1.0 (Apple Message framework v746.2) 4, 16 -- Content-Transfer-Encoding: 7bit 4, 16 -- Message-Id: {5D4D5E4B-7413-405F-8A2F-D0400C40D754-at-umich.edu} 4, 16 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 4, 16 -- To: microscopy-at-microscopy.com 4, 16 -- From: Dorothy Sorenson {dsoren-at-umich.edu} 4, 16 -- Subject: Diamond knives 4, 16 -- Date: Mon, 5 Dec 2005 15:33:10 -0500 4, 16 -- X-Mailer: Apple Mail (2.746.2) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jontes.1-at-osu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jontes.1-at-osu.edu Name: James Jontes
Organization: The Ohio State University
Title-Subject: [Filtered] RMC PoweTome X
Question: Hi, I am planning to buy a routine, room temperature ultramicrotome and am considering the UC6 or the PowerTome X. I don't know that much about RMC, but there is a $15,000 price differential. Is there anything wrong with the RMC? Does anyone have any horror stories?
There was a similar question a while ago, but I couldn't determine the answer from the posts.
James, I have a (now old) RMC7000 and a (also old) Leica Ultracut S. They both have their strengths and weaknesses. Both have needed repairs at one time or another. I have the RMC under a service contract, so its repairs were painless, in terms of cost. I inherited the Leica (I'm actually its 3rd home) and I have had a local service group give it a check up once in a while, and they replaced the mother board in the control unit when it literally went up in smoke earlier this year...very dramatic and not an inexpensive repair. The local service people I use are also the regional reps for the Leica ultra'tomes, so service is usually very quick (as fast as I can get a PO cut..faster if I use a credit card). RMC as a regional service person for the North East. He comes in for the annual PM and if I need service. In terms of performance, the 2 are comparable in my hands. I don't know how the newest models from each company compare to one another. Aside from cost (not an insubstantial consideration, and the reason I have the RMC in the first place), I would try to get some hands-on time on each for you or the person who will be using it most, and get a real sense for the ergonomics, etc. This comment comes from someone with 2 degenerating cervical disks....probably exacerbated by 25+ years of sitting at microtomes and microscopes of various types. I hope this is helpful, Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175 http://www.cornellcelldevbiology.org
==============================Original Headers============================== 1, 21 -- From lcgould-at-med.cornell.edu Tue Dec 6 08:14:40 2005 1, 21 -- Received: from smtp-gw1.med.cornell.edu (smtp-gw1.med.cornell.edu [140.251.3.74]) 1, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB6EEe7N022745 1, 21 -- for {microscopy-at-microscopy.com} ; Tue, 6 Dec 2005 08:14:40 -0600 1, 21 -- Received: from mpx1.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) 1, 21 -- by smtp-gw1.med.cornell.edu (Switch-3.1.7/Switch-3.1.7) with ESMTP id jB6EEblH003728 1, 21 -- for {microscopy-at-microscopy.com} ; Tue, 6 Dec 2005 09:14:38 -0500 (EST) 1, 21 -- Received: from [140.251.145.131] by mpx1.med.cornell.edu 1, 21 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 1, 21 -- with ESMTP id {0IR200A7QY8CLE70-at-mpx1.med.cornell.edu} for 1, 21 -- microscopy-at-microscopy.com; Tue, 06 Dec 2005 09:14:37 -0500 (EST) 1, 21 -- Date: Tue, 06 Dec 2005 09:13:09 -0500 1, 21 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 1, 21 -- Subject: Re: [Microscopy] viaWWW: RMC and PoweTomer X 1, 21 -- In-reply-to: {200512052352.jB5NqWqA001977-at-ns.microscopy.com} 1, 21 -- To: jontes.1-at-osu.edu, Microscopy Listserver {microscopy-at-microscopy.com} 1, 21 -- Message-id: {p06200702bfbb498113a5-at-[140.251.145.131]} 1, 21 -- MIME-version: 1.0 1, 21 -- Content-type: text/plain; charset=us-ascii; format=flowed 1, 21 -- References: {200512052352.jB5NqWqA001977-at-ns.microscopy.com} 1, 21 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.1.0.0, Antispam-Data: 2005.12.6.9 ==============================End of - Headers==============================
A vacancy for a microscopist in the Structural Virology Group of Purdue University is anticipated. This position is ideal for an individual interested in cryo-electron microscopy studies of viruses. The position will involve sample preparation, initial sample assessment, sample freezing, data collection, image analysis, three-dimensional modeling and interpretation. The ideal candidate will be expected to be involved in all aspects of the research including publishing. A BS with a major in biochemistry or a related area of biology and experience with transmission electron microscopy is considered a minimal requirement. Additional backgrounds in physics and computing along with a willingness to learn and the ability to balance multiple projects would be highly desirable. Employment will entail comprehensive training during the first year and extensive daily interactions with a team of graduate students, post-doctoral scholars and faculty. All levels of experience will be considered. Please contact Paul Chipman (765-494-1487, paulrc-at-purdue.edu) for further details.
Purdue University is located in West Lafayette, approximately 70 miles north of Indianapolis and 120 miles south of Chicago. As the major employer in the region with more than 10,000 faculty and staff, Purdue is also home to more than 38,000 students and extends a true "university town" feel to the twin cities of Lafayette and West Lafayette.
Thanks, Paul
Paul Chipman Director, Electron Microscopy Facility Project Leader, Structural Virology EM Studies Dept. of Biology, Purdue University Lilly Hall, Rm. B216 Phone: 765-494-1487 Fax: 765-496-1189
==============================Original Headers============================== 6, 14 -- From paulrc-at-bilbo.bio.purdue.edu Tue Dec 6 08:43:46 2005 6, 14 -- Received: from bilbo.bio.purdue.edu (bilbo.bio.purdue.edu [128.210.155.58]) 6, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB6Ehk2S032065 6, 14 -- for {microscopy-at-microscopy.com} ; Tue, 6 Dec 2005 08:43:46 -0600 6, 14 -- Received: from [128.210.155.233] (dhcp155-233.bio.purdue.edu [128.210.155.233]) 6, 14 -- by bilbo.bio.purdue.edu (Postfix) with ESMTP id 0D654BCF4 6, 14 -- for {microscopy-at-microscopy.com} ; Tue, 6 Dec 2005 09:43:45 -0500 (EST) 6, 14 -- Mime-Version: 1.0 6, 14 -- Message-Id: {a06110402bfbb53539c04-at-[128.210.155.233]} 6, 14 -- Date: Tue, 6 Dec 2005 09:43:44 -0500 6, 14 -- To: microscopy-at-microscopy.com 6, 14 -- From: Paul Chipman {paulrc-at-bilbo.bio.purdue.edu} 6, 14 -- Subject: open position 6, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Have you thought of using AFM in Phase mode instead? I think you would find that it would get around a lot of the sample prep issues and be much more direct.
Hope this is helpful, Barbara Foster
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
P. S. Need a good general reference or light microscopy text for the Spring semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 08:33 AM 12/2/2005, m_mo_shad-at-yahoo.com wrote:
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==============================Original Headers============================== 14, 18 -- From bfoster-at-mme1.com Tue Dec 6 10:03:49 2005 14, 18 -- Received: from 5starpro.com (enterprise.5starpro.com [207.44.136.95]) 14, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB6G3n1k009719 14, 18 -- for {microscopy-at-microscopy.com} ; Tue, 6 Dec 2005 10:03:49 -0600 14, 18 -- Received: (qmail 5691 invoked from network); 6 Dec 2005 10:05:55 -0600 14, 18 -- Received: from host57-99.rancor.birch.net (HELO barbsd505.mme1.com) (65.17.57.99) 14, 18 -- by enterprise.5starpro.com with SMTP; 6 Dec 2005 10:05:55 -0600 14, 18 -- Message-Id: {6.1.2.0.0.20051206100220.0277f418-at-mail.mme1.com} 14, 18 -- X-Sender: bfoster-at-mail.mme1.com 14, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 6.1.2.0 14, 18 -- Date: Tue, 06 Dec 2005 10:03:43 -0600 14, 18 -- To: m_mo_shad-at-yahoo.com, microscopy-at-microscopy.com 14, 18 -- From: Barbara Foster {bfoster-at-mme1.com} 14, 18 -- Subject: Re: [Microscopy] viaWWW: casein micelles 14, 18 -- In-Reply-To: {200512021433.jB2EXDu7014378-at-ns.microscopy.com} 14, 18 -- References: {200512021433.jB2EXDu7014378-at-ns.microscopy.com} 14, 18 -- Mime-Version: 1.0 14, 18 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Gerd- You might be interested In several papers by S.-H. Brorson in the journal Micron, most from the 1990s, all electronically retrievable as PDFs. I recently became interested in these because of some unusual insights (I have never seen these grouped together anywhere else!) that Brorson develops about how raising the content of accelerator 2-8% or of propylene oxide 0-10% can reduce crosslink density of the final cured epoxy, and the relationship of this to rubbery-versus-brittle macro properties, polymer bonding to side-chains of embedded biomolecules, ultramicrotomy cutting quality, post-embedding antigen exposure, and comparison of immuno-labeling with LR White.
He reports that reduced x-linking enhanced "antigen retrieval" (= antigen exposure to post-embedding immunolabels by incubation of sections in hot citrate ) in thin epoxy sections, such that immuno-labeling for larger antigens could become as efficient as in LR White (although he notes less or little success with smaller-smallest antigens).
Micron 29:89-95 (1998) is one of these. However, Micron 35:619-621 (2004) seems to hint that he may have given up the high-accelerator (8%) approach in favor of etching with citrate at 95-145 degrees C and immunostaining at 60°C. I can't tell for sure, so I will copy this message to Brorson himself, hoping for his answer.
You might also be interested in the obscurely published finding (Web of Science does not seem able retrieve it so i can't detect who might have cited it) that quite good structure and antigen survival can be achieved after using uranyl acetate as a primary fixative. (Fassel & Greaser, 1997, Microsc. Res Tech 37:600-601).
-mike reedy-
At 9:58 AM -0600 12/2/05, gerd.leitinger-at-meduni-graz.at wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
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Email: matthems-at-rose-hulman.edu Name: ann
Organization: rose hulman institute of technology
Title-Subject: [Filtered] Required Data
Question: HI Everyone,I am doing a reserach assignment for which i require the values of teh refractive index of mitochondria,nucleus,ribosome,cytoskeleton at various wavelengths.I have tried searching for these values,but have got unsatisfactory results.If anyone knows where i can get such data,could anyone plz help. Thanx
Matt, Most biological macromolecules, whether Lipid, CHO, NA or protein, have a specific refractive increment very near .0018, meaning that each 1.0 g of dry weight dissolved (or suspended) in 100 g of aqueous solution increases the averaged refractive index of that solution or of that volume occupied by the molecular assembly by .0018. Thus if water RI is 1.3300, 1% protein (w/w) is 1.3318. Dry 100% protein would be about 1.58 (1.33 + .185) if specific RI increment is .00185 (behavior is not reliably this linear though at high conc'ns). I learned about all this while doing interference microscopy of myofibrils about 25 year ago. In our lab we still have a usable Vickers M86 scanning microinterferometer microscope that the last user coupled nicely to a Macintosh program, but we haven't found a fundable use for it in the last 20 years.
See Barer and Joseph, Quart J Microsc Sci (1954) 95:399-423, for a discussion of refractometry of living cells and info about the specific refractive increments of solutions of the different macromolecules, varying in reality from perhaps.0014 to .0020. For more references of possible interest, including parts 2 and 3 of Barer and Joseph (1954-55), see the reference list in Joseph, 1981, J. Microscopy, 131:163-172. I'm not sure anyone ever measured isolated organelles except for myofibrils,. You can do it yourself using a phase contrast microscope and irrigating under the coverslip with impermeant immersion medium of graded dilutions to give graded steps in RI, in order to see which RI matches out the contrast of the isolated organelle of interest, making them almost phase-invisible. The two media I used for matching A-bands in myofibrils of insect flight muscle and rabbit psoas were Percoll, and Limulus hemocyanin, washed and finally concentrated by ultracentrifugation in the buffer of choice, then incrementally diluted and subject to RI measurementss in an Abbe refractometer (M. K. Reedy, C. Lucaveche, Adv Exp Med Biol 170:29-45 (1984)). Percoll was more innccuous than hemocyanin on rabbit fibrils; insect fibrils were unchanged by either one.
-mike reedy-
At 3:40 PM -0600 12/6/05, matthems-at-rose-hulman.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
Ann, One more reference ; in a 1966 book Barer wrote a more reader-friendly text on the method that might still give you what you want; I found it a pretty good read. The reference is 1. R. Barer, in Physical Techniques in Biological Research A. W. Pollister, Ed. (Academic Press, New York., 1966) pp. 1-56.
-mike reedy-
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************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
We seek help for the sample prepartion for AFM ananlysis. We are trying to scan the suspended nanoparticles in semicontact mode. Basically We made a smear on glass slide and observed but unable to obtain the good pictures.
Thanks in advance
Regards Shrunali Kulkarni, Scientist Institute of Microbial Technology India
__________________________________________ Yahoo! DSL – Something to write home about. Just $16.99/mo. or less. dsl.yahoo.com
==============================Original Headers============================== 7, 18 -- From aarti_harle-at-yahoo.co.in Wed Dec 7 01:41:05 2005 7, 18 -- Received: from web8305.mail.in.yahoo.com (web8305.mail.in.yahoo.com [202.43.219.217]) 7, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jB77f3Gh008784 7, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Dec 2005 01:41:04 -0600 7, 18 -- Received: (qmail 27124 invoked by uid 60001); 7 Dec 2005 07:41:02 -0000 7, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 18 -- s=s1024; d=yahoo.co.in; 7, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 7, 18 -- b=cMCDcYLKAP4uIwYuBNa9eI5TYLvjrcImQDyjaGDKBJQ9cHCiLlVdytwCUNTm/NbftvWEfKpy1f+t4IlQONrk2ecGxR/IO+RT1A/ZvKodIOMY6acIzNXJ3v/G8JiuDgkQi7e8dwLSN5fzKoLQZCLshb+pbH5Nf6CanUUMZMPPieo= ; 7, 18 -- Message-ID: {20051207074102.27122.qmail-at-web8305.mail.in.yahoo.com} 7, 18 -- Received: from [203.197.210.211] by web8305.mail.in.yahoo.com via HTTP; Tue, 06 Dec 2005 23:41:02 PST 7, 18 -- Date: Tue, 6 Dec 2005 23:41:02 -0800 (PST) 7, 18 -- From: Aarti Harle {aarti_harle-at-yahoo.co.in} 7, 18 -- Subject: AFM sample prepartion 7, 18 -- To: Microscopy-at-Microscopy.Com 7, 18 -- MIME-Version: 1.0 7, 18 -- Content-Type: text/plain; charset=iso-8859-1 7, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: tttan-at-simtech.a-star.edu.sg Name: Tan T. T.
Organization: SIMTech
Title-Subject: [Filtered] Custom Electron Gun developer
Question: Dear all,
I would like to know if anyone has contacts for custom electron gun development.
I need a 120kV field emission electron gun, no scan coil etc. Just the gun alone, with certain specifications.
Would appreciate if you could forward me the names of the companies that do this.
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Email: cheng.huang-at-anu.edu.au Name: Cheng
Organization: CSIRO
Title-Subject: [Filtered] Hard drive in the Link system
Question: Hi,
The hard drive of our old Link x-ray analysis system was crashed recently. We are looking for the replacement. Does anyone by any chance still have this system or hard drive (40 or 80 MB, SCSI) stashed somewhere and won't need anymore? We are happy to pay for it.
They still have one or 2 of the models I bought listed on their webpage - though they are in the UK.
cheng.huang-at-anu.edu.au wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both cheng.huang-at-anu.edu.au as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: cheng.huang-at-anu.edu.au } Name: Cheng } } Organization: CSIRO } } Title-Subject: [Filtered] Hard drive in the Link system } } Question: Hi, } } The hard drive of our old Link x-ray analysis system was crashed recently. We are looking for the replacement. Does anyone by any chance still have this system or hard drive (40 or 80 MB, SCSI) stashed somewhere and won't need anymore? We are happy to pay for it. } } Thanks, } } Cheng } } PI Industry } CSIRO } Australia } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 12 -- From zaluzec-at-microscopy.com Wed Dec 7 07:52:38 2005 } 11, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 11, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB7Dqad0002155 } 11, 12 -- for {microscopy-at-microscopy.com} ; Wed, 7 Dec 2005 07:52:38 -0600 } 11, 12 -- Mime-Version: 1.0 } 11, 12 -- X-Sender: (Unverified) } 11, 12 -- Message-Id: {p06110402bfbc9986fc37-at-[206.69.208.22]} } 11, 12 -- Date: Wed, 7 Dec 2005 07:52:35 -0600 } 11, 12 -- To: microscopy-at-microscopy.com } 11, 12 -- From: cheng.huang-at-anu.edu.au (by way of MicroscopyListserver) } 11, 12 -- Subject: viaWWW: Hard drive for the Link EDS system } 11, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
--
Andy Buckley
AB78-at-ESC.CAM.AC.UK DEPARTMENT OF EARTH SCIENCES UNIVERSITY OF CAMBRIDGE Tel +44 1223 333469 DOWNING STREET +44 1223 333400 CAMBRIDGE FAX +44 1223 333450 CB2 3EQ
Manager of XRD discussion list: http://www.jiscmail.ac.uk/lists/xrd.html
==============================Original Headers============================== 11, 21 -- From ab78-at-esc.cam.ac.uk Wed Dec 7 08:18:32 2005 11, 21 -- Received: from rock.esc.cam.ac.uk (rock.esc.cam.ac.uk [131.111.41.250]) 11, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB7EIWJf022242 11, 21 -- for {microscopy-at-microscopy.com} ; Wed, 7 Dec 2005 08:18:32 -0600 11, 21 -- Received: from andyb.esc.cam.ac.uk ([192.168.17.155]) 11, 21 -- by rock.esc.cam.ac.uk with esmtp (Exim 4.34) 11, 21 -- id 1Ek07r-0002d9-Mc; Wed, 07 Dec 2005 14:18:31 +0000 11, 21 -- Message-ID: {4396EED7.1070102-at-esc.cam.ac.uk} 11, 21 -- Date: Wed, 07 Dec 2005 14:16:55 +0000 11, 21 -- From: Andy Buckley {ab78-at-esc.cam.ac.uk} 11, 21 -- Reply-To: ab78-at-esc.cam.ac.uk 11, 21 -- User-Agent: Mozilla Thunderbird 1.0 (Windows/20041206) 11, 21 -- X-Accept-Language: en-us, en 11, 21 -- MIME-Version: 1.0 11, 21 -- To: microscopy-at-microscopy.com 11, 21 -- CC: cheng.huang-at-anu.edu.au 11, 21 -- Subject: Re: [Microscopy] viaWWW: Hard drive for the Link EDS system 11, 21 -- References: {200512071356.jB7DuFqp013756-at-ns.microscopy.com} 11, 21 -- In-Reply-To: {200512071356.jB7DuFqp013756-at-ns.microscopy.com} 11, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I don't have anything that small anymore. You might check around for some old Macintosh computers and see if you can find a suitable drive. The nice thing about SCSI is that it was a standard. The only rub might be matching up the connector, but I think most drives used the 50-pin rectangular connectors back in that day.
Good luck. Warren Straszheim
-----Original Message----- X-from: cheng.huang-at-anu.edu.au [mailto:cheng.huang-at-anu.edu.au] Sent: Wednesday, December 07, 2005 7:53 AM To: wesaia-at-iastate.edu
Hi, I am looking to acquire one or two WDS spectrometers that fit on any of these JEOL instruments: 733, 840, 6300, 6400, and the 8600. If you have any in your lab that you are not using I'd be very interested to hear from you!
Cheers Mike
-- ******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html ********************************************************************
==============================Original Headers============================== 5, 12 -- From mmcheath-at-mailbox.syr.edu Wed Dec 7 10:09:46 2005 5, 12 -- Received: from mailer.syr.edu (mailer.syr.edu [128.230.18.29]) 5, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB7G9k0m016171 5, 12 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Dec 2005 10:09:46 -0600 5, 12 -- Received: from [128.230.24.90] (www.geochemistry.syr.edu) by mailer.syr.edu (LSMTP for Windows NT v1.1b) with SMTP id {0.14C17796-at-mailer.syr.edu} ; Wed, 7 Dec 2005 11:09:46 -0500 5, 12 -- Mime-Version: 1.0 5, 12 -- Message-Id: {a06110411bfbcb80e2eef-at-[128.230.24.90]} 5, 12 -- Date: Wed, 7 Dec 2005 11:05:50 -0500 5, 12 -- To: Microscopy-at-Microscopy.Com 5, 12 -- From: Michael Cheatham {mmcheath-at-mailbox.syr.edu} 5, 12 -- Subject: uProbe: WDS detectors - availability 5, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Spin-coating would be helpful. I used to spin coat magnetic nanoparticles onto newly cleaved mica substrate. The results were amazing; there almost were not big aggregates on the substrate.
Alternatively, you may try direct deposition of your suspension on the substrate. However, in this case you have to remove excess solvent by rinsing it with the solvent used in your sample preparation. I successfully got individually distributed magnetic nanoparticles on mica, too.
Wish this helps,
Susheng Department of Chemistry Oklahoma State University Stillwater, OK 74075
--- aarti_harle-at-yahoo.co.in wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello } } We seek help for the sample prepartion for AFM } ananlysis. } We are trying to scan the suspended nanoparticles in } semicontact mode. Basically We made a smear on glass } slide and observed but unable to obtain the good } pictures. } } Thanks in advance } } Regards } Shrunali Kulkarni, Scientist } Institute of Microbial Technology } India } } } } __________________________________________ } Yahoo! DSL – Something to write home about. } Just $16.99/mo. or less. } dsl.yahoo.com } } } ==============================Original } Headers============================== } 7, 18 -- From aarti_harle-at-yahoo.co.in Wed Dec 7 } 01:41:05 2005 } 7, 18 -- Received: from web8305.mail.in.yahoo.com } (web8305.mail.in.yahoo.com [202.43.219.217]) } 7, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with
In the past I have used PVA dissolved in EtOH as an adhesive. (i.e., spread the liquid on a surface, and let dry ... which would leave a very thin film that would become tacky when heated).
That was then ... and now I'm not sure what "PVA" is. I thought it was polyvinyl alcohol, but it may be polyvinyl acetate. If anyone knows, please remind me ... and provide a source or manufacturer if possible. (I also note that Sigma-Aldrich lists 3 different PV acetates, differeng in mol.wt.)
PVA stands for polyvinylacetate. PVAH is usually used to distinguish polyvinylalcohol although people tend to be lax on the terminology. The acetate my be hydrolyzed to produce the alcohol. Commercial products often contain partially hydrolyzed versions in order to tailor their properties to a particular use, so both species may be present. If you look at the various PVA and PVAH spectra in an infrared database, you will often find them arranged both by molecular weight and by degree of hydrolysis, that is, by the proportion of esterified to hydroxylated units. As you would expect, the intensities of the hydroxyl and ester carbonyl peaks vary tremendously from 100% PVA to 100% PVAH. The greater the PVAH content, the less the polar organic solvents will dissolve it and the more readily it will dissolve in hydrogen-bonding solvents like alcohols. The common form of "white glue" is an aqueous dispersion of PVA colloids rather than a true solution and when dried cannot be readily redispersed. Unless the molecular weight is small, most PVAs are not soluble in large amounts in any single solvent. Methyl ethyl ketone tends to work best. If you have used ethanol in the past it sounds like the material that you were working with may have been largely PVAH.
John Twilley
-----Original Message----- X-from: michael-at-Shaffer.net Sent: Dec 7, 2005 11:50 AM To: jtwilley-at-sprynet.com
In the past I have used PVA dissolved in EtOH as an adhesive. (i.e., spread the liquid on a surface, and let dry ... which would leave a very thin film that would become tacky when heated).
That was then ... and now I'm not sure what "PVA" is. I thought it was polyvinyl alcohol, but it may be polyvinyl acetate. If anyone knows, please remind me ... and provide a source or manufacturer if possible. (I also note that Sigma-Aldrich lists 3 different PV acetates, differeng in mol.wt.)
---------------------------------------------------------------------- | FOCUS ON MICROSCOPY 2006 -- Perth, Australia -- April 9-12, 2006 | ----------------------------------------------------------------------
19th International Conference on 3D Image Processing in Microscopy 18th International Conference on Confocal Microscopy
Dear Colleagues,
We would like to announce that about 15 fee waivers will be available for students for attending the upcoming Focus on Microscopy conference courtesy of the generous support of the Australian Microscopy and Microanalysis Society.
For details see the website http://www.FocusOnMicroscopy.org at "student bursaries". The deadline for bursary application is Jan. 3, 2006.
As the next in a series of unique interdisciplinary meetings on advanced multidimensional light microscopy and image processing, the FOM 2006 conference will be hosted by the University of Western Australia in Perth. The conference will be located at the beautiful Esplanade Hotel in the historic waterfront suburb of Perth, Fremantle.
Focus on Microscopy 2006 is the continuation of a successful conference series presenting the latest innovations in optical microscopy and its applications in biology, medicine, material science, and information storage. 3D optical imaging and related theory are important subjects for the conference. The series is as relevant now as at any time in its history as the scientific and engineering communities strive to meet the needs of a surging life sciences sector, as well as respond to the sustained pressure for miniaturization in lithography and data storage.
The conference series is known for covering the rapid development of advanced fluorescence labeling techniques for the confocal and multi-photon 3D imaging of -live- biological specimens. This year, in addition, special attention will be given to imaging in thick tissues and the use of laser light as an active tool at sub-micrometer length scales for cell biology, nanobiotechnology, and medicine.
Abstracts for contributions are invited and can already be submitted through the website: http://www.FocusOnMicroscopy.org where further information on the present and previous FOM conferences can be found.
Important dates:
- Deadline for the submission of abstracts: January 9, 2006 - Acceptance of contributions and draft program: January 23, 2006 - Deadline for early registration: February 20, 2006
Welcoming you to beautiful Perth for the FOM2006 conference and exhibition.
On behalf of the organizing committee,
- David Sampson, University of Western Australia, Perth, Australia - Fred Brakenhoff, Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands -- E-mail: info2006-at-FocusOnMicroscopy.org Web: www.FocusOnMicroscopy.org
-- Prof. Dr. G.J. Brakenhoff Section of Molecular Cytology Centre for Advanced Microscopy Swammerdam Institute for Life Sciences University of Amsterdam Kruislaan 316 P.O.Box 94062 1090 GB Amsterdam Tel.: +31 - 20 – 525 5189 Fax.: +31 - 20 - 525 6271 e-mail: brakenhoff-at-science.uva.nl
-- Prof. Dr. G.J. Brakenhoff Section of Molecular Cytology Centre for Advanced Microscopy Swammerdam Institute for Life Sciences University of Amsterdam Kruislaan 316 P.O.Box 94062 1090 GB Amsterdam Tel.: +31 - 20 – 525 5189 Fax.: +31 - 20 - 525 6271 e-mail: brakenhoff-at-science.uva.nl
==============================Original Headers============================== 27, 30 -- From brakenho-at-science.uva.nl Thu Dec 8 03:33:14 2005 27, 30 -- Received: from smtp.science.uva.nl (smtp.science.uva.nl [146.50.4.84]) 27, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB89XDk1001391 27, 30 -- for {microscopy-at-msa.microscopy.com} ; Thu, 8 Dec 2005 03:33:13 -0600 27, 30 -- Received: from webmail.science.uva.nl [146.50.4.91] 27, 30 -- by smtp.science.uva.nl with ESMTP (sendmail 8.11.6p2/config 11.36). 27, 30 -- id jB89X8C04860; Thu, 8 Dec 2005 10:33:08 +0100 27, 30 -- Received: from localhost 27, 30 -- by webmail.science.uva.nl (sendmail 8.11.6/config 11.35). 27, 30 -- id jB89X7Q00300; Thu, 8 Dec 2005 10:33:07 +0100 27, 30 -- X-Organisation: Faculty of Science, University of Amsterdam, The Netherlands 27, 30 -- X-URL: http://www.science.uva.nl/ 27, 30 -- Received: from localhost ([127.0.0.1]) 27, 30 -- (SquirrelMail authenticated user brakenho); 27, 30 -- by webmail.science.uva.nl with HTTP; 27, 30 -- Thu, 8 Dec 2005 10:33:07 +0100 (CET) 27, 30 -- Message-ID: {49348.145.18.160.47.1134034387.squirrel-at-145.18.160.47} 27, 30 -- Date: Thu, 8 Dec 2005 10:33:07 +0100 (CET) 27, 30 -- Subject: FOM 2006, Student Bursaries, Focus on Microscopy 2006, Perth, 27, 30 -- Australia, April 9-12, 2006 27, 30 -- From: "Fred Brakenhoff" {brakenho-at-science.uva.nl} 27, 30 -- To: microscopy-at-msa.microscopy.com 27, 30 -- User-Agent: SquirrelMail/1.4.3a 27, 30 -- X-Mailer: SquirrelMail/1.4.3a 27, 30 -- MIME-Version: 1.0 27, 30 -- Content-Type: text/plain;charset=iso-8859-1 27, 30 -- Content-Transfer-Encoding: 8bit 27, 30 -- X-Priority: 3 (Normal) 27, 30 -- Importance: Normal 27, 30 -- X-Virus-Scanned: by amavisd-new ==============================End of - Headers==============================
I would recommend to use freshly cleaved mica as a substrate in place of a glass slide. The topography of glass slides can be as large as several 10nm if not some 100nm. Probably you will not be able to distinguish your nanopartilces on such a substrate.
Best regards,
Petra
--------------------------------------- Dr. Petra Wahlbring Goodyear S.A. Technical Center Analytical Test Laboratories L-7750 Colmar-Berg Luxembourg Tel +352 8199 3725 Fax +352 8199 3905 e-mail: petra.wahlbring-at-goodyear.com
aarti_harle-at-yahoo .co.in To 12/07/05 08:46 AM petra.wahlbring-at-goodyear.com cc
Please respond to Subject aarti_harle-at-yahoo [Microscopy] AFM sample prepartion .co.in
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Hello
We seek help for the sample prepartion for AFM ananlysis. We are trying to scan the suspended nanoparticles in semicontact mode. Basically We made a smear on glass slide and observed but unable to obtain the good pictures.
Thanks in advance
Regards Shrunali Kulkarni, Scientist Institute of Microbial Technology India
==============================Original Headers============================== 23, 15 -- From petra.wahlbring-at-goodyear.com Thu Dec 8 03:36:53 2005 23, 15 -- Received: from eclngm01.ec.goodyear.com (eclngm01.ec.goodyear.com [57.67.177.2] (may be forged)) 23, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB89aqOY002035 23, 15 -- for {Microscopy-at-Microscopy.Com} ; Thu, 8 Dec 2005 03:36:53 -0600 23, 15 -- In-Reply-To: {200512070746.jB77kAaH016708-at-ns.microscopy.com} 23, 15 -- Subject: Re: [Microscopy] AFM sample prepartion 23, 15 -- To: aarti_harle-at-yahoo.co.in, Microscopy-at-Microscopy.Com 23, 15 -- X-Mailer: Lotus Notes Release 6.5 September 26, 2003 23, 15 -- Message-ID: {OFB3509155.303AAC19-ONC12570D1.0033A249-C12570D1.0034C198-at-goodyear.com} 23, 15 -- From: petra.wahlbring-at-goodyear.com 23, 15 -- Date: Thu, 8 Dec 2005 10:36:13 +0100 23, 15 -- X-MIMETrack: Serialize by Router on ECLNGM01/EU/GDYRNET(Release 6.5.2|June 01, 2004) at 23, 15 -- 12/08/2005 10:36:54 AM 23, 15 -- MIME-Version: 1.0 23, 15 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Henrik.Kaker-at-guest.arnes.si as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Henrik.Kaker-at-guest.arnes.si Name: Henrik Kaker
Organization: SEM-EDS Lab
Title-Subject: [Filtered] Image distortion in Jeol JSM 35-CF
Question: Good Morning, All
In our old SEM we found image distortion. Please see the image of Cu grid at http://www.kaker.com/300x.jpg. Can anyone help me to correct this error in our SEM. Any assistance available is greatly appreciated.
EXTENDED ABSTRACT DEADLINE FOR STUDENT POSTER COMPETITION
To be held in conjunction with the March 24, 2006 meeting of the Midwest
Microscopy and Microanalysis Society
Affiliate of the Microscopy Society of America and the Microbeam Analysis Society of America
NEW ABSTRACT DEADLINE: Abstracts must be received in electronic format by 5PM, Monday, January 16, 2006.
The first quarter meeting of the Midwest Microscopy and Microanalysis Society will be held on Friday, March 24, 2006, in the Pancoe Life Sciences Building, Northwestern University, Evanston, Illinois. A student poster competition open to undergraduate and graduate students will be held in conjunction with the meeting. Posters should illustrate utilization of microscopy for either biological or materials science study. Prizes will be awarded as follows:
$300 for 1st place $200 for 2nd place $100 for 3rd place
Abstracts must be received in electronic format by 5PM on Monday, January 16, 2006. To be eligible for a prize, you must be first author on the poster, and you must be present at the meeting. You are encouraged to submit your entry as early as possible, as space may be limited. Abstracts from last year's competition, and an example of the judging worksheet can be found on the MMMS website:
-----Original Message----- X-from: Henrik.Kaker-at-guest.arnes.si [mailto:Henrik.Kaker-at-guest.arnes.si] Sent: Thursday, December 08, 2005 9:15 AM To: White, Woody N.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both Henrik.Kaker-at-guest.arnes.si as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: Henrik.Kaker-at-guest.arnes.si Name: Henrik Kaker
Organization: SEM-EDS Lab
Title-Subject: [Filtered] Image distortion in Jeol JSM 35-CF
Question: Good Morning, All
In our old SEM we found image distortion. Please see the image of Cu grid at http://www.kaker.com/300x.jpg. Can anyone help me to correct this error in our SEM. Any assistance available is greatly appreciated.
Check that the specimen is grounded. The stage grounding wire may have come loose or become broken. Confirm by directly grounding the specimen holder inside the chamber.
The other cause could be loss of AC line sync.
gary g.
At 06:28 AM 12/8/2005, you wrote:
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==============================Original Headers============================== 9, 25 -- From gary-at-gaugler.com Thu Dec 8 10:07:00 2005 9, 25 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jB8G6xuR019512 9, 25 -- for {microscopy-at-microscopy.com} ; Thu, 8 Dec 2005 10:06:59 -0600 9, 25 -- Received: (qmail 931 invoked from network); 8 Dec 2005 08:05:55 -0800 9, 25 -- Received: by simscan 1.1.0 ppid: 916, pid: 917, t: 3.3479s 9, 25 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1095 spam: 3.0.3 9, 25 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 9, 25 -- by qsmtp1 with SMTP; 8 Dec 2005 08:05:51 -0800 9, 25 -- Message-Id: {6.2.3.4.2.20051208080518.0298e580-at-mail.calweb.com} 9, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 9, 25 -- Date: Thu, 08 Dec 2005 08:06:56 -0800 9, 25 -- To: Henrik.Kaker-at-guest.arnes.si 9, 25 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 25 -- Subject: Re: [Microscopy] viaWWW: Image distortion in Jeol JSM 35-CF 9, 25 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 25 -- In-Reply-To: {200512081428.jB8ESt9w026925-at-ns.microscopy.com} 9, 25 -- References: {200512081428.jB8ESt9w026925-at-ns.microscopy.com} 9, 25 -- Mime-Version: 1.0 9, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 9, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on 9, 25 -- qsmtp1.mc.surewest.net 9, 25 -- X-Spam-Level: 9, 25 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 9, 25 -- version=3.0.3 ==============================End of - Headers==============================
Hi, a friend (mhg25-at-cam.ac.uk) is considering purchasing a low energy ion mill (e.g. the 'Gentle Mill', or the Fischione machine). Does anyone have any experience (good or bad) that they would like to share on the technique and the machines, which would help?
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==============================Original Headers============================== 7, 29 -- From richard.beanland-at-bookham.com Thu Dec 8 10:43:05 2005 7, 29 -- Received: from mail78.messagelabs.com (mail78.messagelabs.com [195.245.230.131]) 7, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jB8Gh3SN026765 7, 29 -- for {microscopy-at-microscopy.com} ; Thu, 8 Dec 2005 10:43:04 -0600 7, 29 -- X-VirusChecked: Checked 7, 29 -- X-Env-Sender: richard.beanland-at-bookham.com 7, 29 -- X-Msg-Ref: server-11.tower-78.messagelabs.com!1134060179!43966272!1 7, 29 -- X-StarScan-Version: 5.5.9.1; banners=bookham.com,-,- 7, 29 -- X-Originating-IP: [213.249.209.179] 7, 29 -- Received: (qmail 11863 invoked from network); 8 Dec 2005 16:43:01 -0000 7, 29 -- Received: from unknown (HELO cas-smx-brdg-01.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 7, 29 -- by server-11.tower-78.messagelabs.com with SMTP; 8 Dec 2005 16:43:01 -0000 7, 29 -- Content-class: urn:content-classes:message 7, 29 -- MIME-Version: 1.0 7, 29 -- Content-Type: text/plain; 7, 29 -- charset="iso-8859-1" 7, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 7, 29 -- Subject: Low energy ion milling 7, 29 -- Date: Thu, 8 Dec 2005 16:42:59 -0000 7, 29 -- Message-ID: {FF719652ABB4414D86916ACAAE5BBB24015FBED5-at-cas-smx-01.caswell1.europe.bkhm.net} 7, 29 -- X-MS-Has-Attach: 7, 29 -- X-MS-TNEF-Correlator: 7, 29 -- Thread-Topic: Low energy ion milling 7, 29 -- Thread-Index: AcX8Frizg6zsWvWNT9Wx1tgVzKkazQ== 7, 29 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 7, 29 -- To: {microscopy-at-microscopy.com} 7, 29 -- Cc: {mhg25-at-cam.ac.uk} 7, 29 -- Content-Transfer-Encoding: 8bit 7, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jB8Gh3SN026765 ==============================End of - Headers==============================
Hi all, Nestor, I hope I'm not violating any rules with this...
I just received this message from Sigma Xi:
SURPLUS EQUIPMENT FOR HURRICANE VICTIMS Do you have any surplus lab or research-related scientific equipment you could offer to university or other research labs affected by the hurricanes? You can list it on Sigma Xi Exchange at http://exchange.sigmaxi.org You can also search for equipment that has been listed.
Rather than setting up redundant services, perhaps anyone who can help can use this link.
Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175 http://www.cornellcelldevbiology.org
==============================Original Headers============================== 5, 21 -- From lcgould-at-med.cornell.edu Thu Dec 8 11:18:10 2005 5, 21 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45]) 5, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB8HI9sU002549 5, 21 -- for {microscopy-at-microscopy.com} ; Thu, 8 Dec 2005 11:18:09 -0600 5, 21 -- Received: from mpx2.med.cornell.edu (pc113142-10.med.cornell.edu [140.251.11.119]) 5, 21 -- by smtp-gw2.med.cornell.edu (Switch-3.1.7/Switch-3.1.7) with ESMTP id jB8HI2RZ026059 5, 21 -- for {microscopy-at-microscopy.com} ; Thu, 8 Dec 2005 12:18:08 -0500 (EST) 5, 21 -- Received: from [140.251.145.131] by mpx2.med.cornell.edu 5, 21 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 5, 21 -- with ESMTP id {0IR60035DW211R60-at-mpx2.med.cornell.edu} for 5, 21 -- microscopy-at-microscopy.com; Thu, 08 Dec 2005 12:18:02 -0500 (EST) 5, 21 -- Date: Thu, 08 Dec 2005 12:16:33 -0500 5, 21 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 5, 21 -- Subject: Re: surplus equipment for hurricane victims 5, 21 -- In-reply-to: {200512081653.jB8GrHBJ029463-at-ns.microscopy.com} 5, 21 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 5, 21 -- Message-id: {p06200707bfbe1a480237-at-[140.251.145.131]} 5, 21 -- MIME-version: 1.0 5, 21 -- Content-type: text/plain; charset=us-ascii; format=flowed 5, 21 -- References: {200512081653.jB8GrHBJ029463-at-ns.microscopy.com} 5, 21 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.1.0.0, Antispam-Data: 2005.12.8.15 ==============================End of - Headers==============================
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This is an entirely personal opinion ...
If I am misunderstanding this, I apologise - I'm British and the USA is a foreign country as far as I'm concerned, with a culture I don't pretend to fully understand.
And sorry, I'm probably violating list rules, hoiwever, I guess I can live with being excluded, if Nestor considers it necessary, but what on earth are colleges and laboratories in the richest and most powerful nation on the planet doing asking for donations?
Mexico, Cuba, Dominican Republic, Haiti and other Carribean nations, I could understand but come on, please, the USA, the wealthiest nation on the planet?
If you've got equipment to donate, send it to somewhere it really is needed.
-- Larry Stoter
PLEASE NOTE 1. Any mail other than plain text will be automatically deleted. 2. Any mail, legitimate or not, apparently or actually from hotmail, netscape, yahoo or excite will automatically be deleted. 3. Mail with no subject or without a clear subject will be ignored :-)
==============================Original Headers============================== 8, 16 -- From larry-at-cymru.freewire.co.uk Thu Dec 8 14:56:20 2005 8, 16 -- Received: from get.freewire.net (get.freewire.net [195.184.229.250]) 8, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB8KuItB019070 8, 16 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 8 Dec 2005 14:56:19 -0600 8, 16 -- Received: from [217.154.250.75] (th6dc-217-154-250-75.dial.mistral.co.uk [217.154.250.75] (may be forged)) 8, 16 -- by get.freewire.net (8.11.6/8.11.6) with ESMTP id jB8KtrQ08156; 8, 16 -- Thu, 8 Dec 2005 20:55:53 GMT 8, 16 -- Mime-Version: 1.0 8, 16 -- Message-Id: {p06210203bfbe4b076896-at-[217.154.255.34]} 8, 16 -- In-Reply-To: {200512081738.jB8HcIcZ007737-at-ns.microscopy.com} 8, 16 -- References: {200512081738.jB8HcIcZ007737-at-ns.microscopy.com} 8, 16 -- Date: Thu, 8 Dec 2005 20:55:56 +0000 8, 16 -- To: lcgould-at-med.cornell.edu, Microscopy-at-MSA.Microscopy.Com 8, 16 -- From: Larry Stoter {larry-at-cymru.freewire.co.uk} 8, 16 -- Subject: [Microscopy] Re: surplus equipment for hurricane victims 8, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Hi all I wanted to know what sort of fees various labs charge their users for services rendered. We are going to be able to offer edx and eels in the near future and we were wondering what sort of schemes other labs use for charging in house and external clients. I am wondering what other labs charge for: straight beam time, specimen prep, training, pictures (yes we still use film!) edx, eels etc for in house and external clients and whether some of these things are lumped together (for instance are pictures covered in the beam time price etc). Thanks in advance!
Rod Nicholls Histology/Electron Microscopy Technician Department of Anatomy and Cell Biology Queen's University Kingston, Ontario K7L 3N6 Phone: 613- 533 6000 x 78265
==============================Original Headers============================== 3, 15 -- From nicholls-at-post.queensu.ca Thu Dec 8 15:31:18 2005 3, 15 -- Received: from post.queensu.ca (post.QueensU.CA [130.15.126.6]) 3, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB8LVGCs025657 3, 15 -- for {microscopy-at-microscopy.com} ; Thu, 8 Dec 2005 15:31:16 -0600 3, 15 -- Received: from EMSuite.post.queensu.ca (U55.N149.QueensU.CA [130.15.149.55]) 3, 15 -- by post.queensu.ca (8.13.1/8.13.1) with ESMTP id jB8LVE3x026106 3, 15 -- for {microscopy-at-microscopy.com} ; Thu, 8 Dec 2005 16:31:14 -0500 (EST) 3, 15 -- Message-Id: {6.2.3.4.0.20051208153649.01cb7ff8-at-post.queensu.ca} 3, 15 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 3, 15 -- Date: Thu, 08 Dec 2005 16:31:08 -0500 3, 15 -- To: microscopy-at-microscopy.com 3, 15 -- From: Rod Nicholls {nicholls-at-post.queensu.ca} 3, 15 -- Subject: EM fees 3, 15 -- Mime-Version: 1.0 3, 15 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Larry, I'm replying off the list so as not to start a flame-throwing barrage. There may be one anyway, but I won't have contributed to it.... The area struck by Hurricane Katrina suffered severe damage. All of Tulane University is being housed by and taught at other institutions, and that's just one of the schools in the area. Also, most of the research at those schools is funded by money from the NIH and other granting institutions. The NIH funds come from US tax dollars, and the schools will be hard-pressed to replace much of their equipment when so much of their ready cash must be directed simply to making the buildings habitable/functional again. Usually, the US schools are generous about sending excess/older equipment out of the country. I know that Weill, where I am has sent things to Columbia and other SA countries in the past. This is a unique situation. Major portions of 3 states were devastated. To put it in terms you might better appreciate: imaging England, France and Germany being wiped out by a natural disaster. Where you guys have individual nations, we have states. Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175 http://www.cornellcelldevbiology.org
==============================Original Headers============================== 1, 22 -- From lcgould-at-med.cornell.edu Thu Dec 8 16:15:27 2005 1, 22 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45]) 1, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB8MFRGX003375 1, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 8 Dec 2005 16:15:27 -0600 1, 22 -- Received: from mpx1.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) 1, 22 -- by smtp-gw2.med.cornell.edu (Switch-3.1.7/Switch-3.1.7) with ESMTP id jB8MFOG7001681 1, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 8 Dec 2005 17:15:24 -0500 (EST) 1, 22 -- Received: from [140.251.145.131] by mpx1.med.cornell.edu 1, 22 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 1, 22 -- with ESMTP id {0IR7006PI9TN4S20-at-mpx1.med.cornell.edu} for 1, 22 -- Microscopy-at-MSA.Microscopy.Com; Thu, 08 Dec 2005 17:15:24 -0500 (EST) 1, 22 -- Date: Thu, 08 Dec 2005 17:13:55 -0500 1, 22 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 1, 22 -- Subject: [Microscopy] Re: surplus equipment for hurricane victims 1, 22 -- In-reply-to: {p06210203bfbe4b076896-at-[217.154.255.34]} 1, 22 -- To: Larry Stoter {larry-at-cymru.freewire.co.uk} , Microscopy-at-MSA.Microscopy.Com 1, 22 -- Message-id: {p0620070dbfbe5eea1829-at-[140.251.145.131]} 1, 22 -- MIME-version: 1.0 1, 22 -- Content-type: text/plain; charset=us-ascii; format=flowed 1, 22 -- References: {200512081738.jB8HcIcZ007737-at-ns.microscopy.com} 1, 22 -- {p06210203bfbe4b076896-at-[217.154.255.34]} 1, 22 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.1.0.0, Antispam-Data: 2005.12.8.26 ==============================End of - Headers==============================
Rod Nicholls wrote: ============================================== Hi all I wanted to know what sort of fees various labs charge their users for services rendered. We are going to be able to offer edx and eels in the near future and we were wondering what sort of schemes other labs use for charging in house and external clients. I am wondering what other labs charge for: straight beam time, specimen prep, training, pictures (yes we still use film!) edx, eels etc for in house and external clients and whether some of these things are lumped together (for instance are pictures covered in the beam time price etc). Thanks in advance! ============================================== There are certainly laws in the USA and I believe also in Canada and in most other countries that would make illegal competitors of a class from sitting down and discussing their fees and selling prices. Most governemental legal systems see this as "price fixing". I would respectfully suggest this should not be an appropriate topic for discussion on this listserver.
A more appropriate kind of discussion might relate to what would and would not constitute appropriate use of the institutional facilities for external users. After all, universities should not be in direct competition with for-profit tax-paying commerical firms. It might happen in certain university environments, but that does not make it right.
This does not mean that universities should not be doing work for external users. It only means that such work should be restricted to that which is consistent with educational objectives, namely that the work is basic and fundamental in nature, the results would be intended for prompt publication and the results would be suitable for inclusion in a student's thesis. Excluded would be work the results from which would become the private property of the sponsoring customer, would not be intended for prompt publication and would not be appropriate for inclusion in a student's thesis.
I would respectfully suggest that discussions about fees among those of us who privide services for a fee should not be a part of this listserver.
Disclaimer: For the 35+ years of the existence of my firm, I have had to face unfair competition from certain universities and other tax-exempt entities. So indeed I do have a vested interest in seeing that unviersities do not enter the marketplace of services that take unfair advantages of their tax status. Numerous independent laboratories exist globally to provide such services in the private sector, for example of some, see URL http://www.2spi.com/catalog/hot-service7.html
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President Structure Probe, Inc. FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
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==============================Original Headers============================== 12, 26 -- From cgarber-at-2spi.com Thu Dec 8 19:18:59 2005 12, 26 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 12, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB91IwqH016543 12, 26 -- for {microscopy-at-msa.microscopy.com} ; Thu, 8 Dec 2005 19:18:58 -0600 12, 26 -- Received: from Desktop ([71.225.125.97]) 12, 26 -- (authenticated bits=0) 12, 26 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id jB91IqHQ021366 12, 26 -- for {microscopy-at-msa.microscopy.com} ; Thu, 8 Dec 2005 20:18:58 -0500 12, 26 -- X-IDV-FirstRcvd: [71.225.125.97] 12, 26 -- X-IDV-HELO: Desktop 12, 26 -- X-IDV-Authenticated-User: cgarber 12, 26 -- Message-ID: {00c101c5fc5e$8a423f90$6301a8c0-at-Desktop} 12, 26 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 12, 26 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 12, 26 -- Subject: Lab fees 12, 26 -- Date: Thu, 8 Dec 2005 20:19:01 -0500 12, 26 -- MIME-Version: 1.0 12, 26 -- Content-Type: text/plain; 12, 26 -- format=flowed; 12, 26 -- charset="iso-8859-1"; 12, 26 -- reply-type=original 12, 26 -- Content-Transfer-Encoding: 7bit 12, 26 -- X-Priority: 3 12, 26 -- X-MSMail-Priority: Normal 12, 26 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2670 12, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2670 ==============================End of - Headers==============================
Lee Some numbers might help. Germany has only 50% of the land area of Texas (we all know it's big) but has 30% more population. The total population of the three countries you mention (191 million) is about three times the total population of the Gulf states Texas, Missouri, Georgia, Louisiana, Alabama, Florida (~64 million), living in about a quarter of the area.
Chris
----- Original Message ----- X-from: {lcgould-at-med.cornell.edu} To: {cjeffree-at-staffmail.ed.ac.uk} Sent: Thursday, December 08, 2005 10:20 PM
OOPS! I really did think that I'd wiped the list's address from my reply...sorry. Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175 http://www.cornellcelldevbiology.org
==============================Original Headers============================== 1, 21 -- From lcgould-at-med.cornell.edu Fri Dec 9 08:04:05 2005 1, 21 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45]) 1, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9E44TO011994 1, 21 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 08:04:05 -0600 1, 21 -- Received: from mpx1.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) 1, 21 -- by smtp-gw2.med.cornell.edu (Switch-3.1.7/Switch-3.1.7) with ESMTP id jB9E43OL000474 1, 21 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 09:04:04 -0500 (EST) 1, 21 -- Received: from [140.251.145.131] by mpx1.med.cornell.edu 1, 21 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 1, 21 -- with ESMTP id {0IR800J2BHQPQV40-at-mpx1.med.cornell.edu} for 1, 21 -- microscopy-at-microscopy.com; Fri, 09 Dec 2005 09:04:03 -0500 (EST) 1, 21 -- Date: Fri, 09 Dec 2005 09:02:34 -0500 1, 21 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 1, 21 -- Subject: Re: [Microscopy] Re: surplus equipment for hurricane victims 1, 21 -- In-reply-to: {43997908.20295.FB0644-at-localhost} 1, 21 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 1, 21 -- Message-id: {p06200702bfbf3eaa890d-at-[140.251.145.131]} 1, 21 -- MIME-version: 1.0 1, 21 -- Content-type: text/plain; charset=us-ascii; format=flowed 1, 21 -- References: {43997908.20295.FB0644-at-localhost} 1, 21 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.1.0.0, Antispam-Data: 2005.12.9.10 ==============================End of - Headers==============================
Yes of course, apologies for my dyslexia, I meant Mississippi
Setting geography aside, since Lee's cat is now out of the bag on this question of used/surplus equipment, it might be of interest to know what aid is being requested and proposed. I expect many would help out if they knew how to, and what resources are required.
Chris
----- Original Message ----- X-from: "Debby Sherman" {dsherman-at-purdue.edu} To: {c.jeffree-at-ed.ac.uk} Sent: Friday, December 09, 2005 1:40 PM
} This is a } unique situation. Major portions of 3 states were devastated. To } put it in terms you might better appreciate: imaging England, France } and Germany being wiped out by a natural disaster.
It's a long way out of proportions.
Vladimir
==============================Original Headers============================== 6, 23 -- From DusevichV-at-umkc.edu Fri Dec 9 09:11:32 2005 6, 23 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10]) 6, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9FBUef022457 6, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 9 Dec 2005 09:11:31 -0600 6, 23 -- Received: from KC-MAIL2.kc.umkc.edu ([134.193.32.1]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.1830); 6, 23 -- Fri, 9 Dec 2005 09:11:29 -0600 6, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 6, 23 -- Content-class: urn:content-classes:message 6, 23 -- MIME-Version: 1.0 6, 23 -- Content-Type: text/plain; 6, 23 -- charset="us-ascii" 6, 23 -- Subject: RE: [Microscopy] Re: surplus equipment for hurricane victims 6, 23 -- Date: Fri, 9 Dec 2005 09:11:29 -0600 6, 23 -- Message-ID: {4BF01C49DF1C4F4E883C3E25C670874933FF59-at-KC-MAIL1.kc.umkc.edu} 6, 23 -- X-MS-Has-Attach: 6, 23 -- X-MS-TNEF-Correlator: 6, 23 -- Thread-Topic: [Microscopy] Re: surplus equipment for hurricane victims 6, 23 -- Thread-Index: AcX8RlDJ7sXF59SfTFq34CKiNxzv6gAjGJBw 6, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 6, 23 -- To: {Microscopy-at-MSA.Microscopy.Com} 6, 23 -- X-OriginalArrivalTime: 09 Dec 2005 15:11:29.0952 (UTC) FILETIME=[D5296A00:01C5FCD2] 6, 23 -- Content-Transfer-Encoding: 8bit 6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jB9FBUef022457 ==============================End of - Headers==============================
} This just sounds like a little market research to me. } ---------------------------------------------------------------------------- } } Rod Nicholls wrote: } ============================================== } Hi all } I wanted to know what sort of fees various labs charge their users } for services rendered. We are going to be able to offer edx and eels } in the near future and we were wondering what sort of schemes other } labs use for charging in house and external clients. I am wondering } what other labs charge for: straight beam time, specimen prep, } training, pictures (yes we still use film!) edx, eels etc for in } house and external clients and whether some of these things are } lumped together (for instance are pictures covered in the beam time price } etc). } Thanks in advance! } ============================================== } There are certainly laws in the USA and I believe also in Canada and in most } other countries that would make illegal competitors of a class from sitting } down and discussing their fees and selling prices. Most governemental legal } systems see this as "price fixing". I would respectfully suggest this } should not be an appropriate topic for discussion on this listserver. } } A more appropriate kind of discussion might relate to what would and would } not constitute appropriate use of the institutional facilities for external } users. After all, universities should not be in direct competition with } for-profit tax-paying commerical firms. It might happen in certain } university environments, but that does not make it right. } } This does not mean that universities should not be doing work for external } users. It only means that such work should be restricted to that which is } consistent with educational objectives, namely that the work is basic and } fundamental in nature, the results would be intended for prompt publication } and the results would be suitable for inclusion in a student's thesis. } Excluded would be work the results from which would become the private } property of the sponsoring customer, would not be intended for prompt } publication and would not be appropriate for inclusion in a student's } thesis. } } I would respectfully suggest that discussions about fees among those of us } who privide services for a fee should not be a part of this listserver. } } Disclaimer: For the 35+ years of the existence of my firm, I have had to } face unfair competition from certain universities and other tax-exempt } entities. So indeed I do have a vested interest in seeing that unviersities } do not enter the marketplace of services that take unfair advantages of } their tax status. Numerous independent laboratories exist globally to } provide such services in the private sector, for example of some, see URL } http://www.2spi.com/catalog/hot-service7.html } } Chuck } } ============================================ } } Charles A. Garber, Ph. D. Ph: 1-610-436-5400 } President } Structure Probe, Inc. FAX: 1-610-436-5755 } PO BOX 656 e-mail:cgarber-at-2spi.com } West Chester, PA 19381-0656 USA } Cust.Service: spi2spi-at-2spi.com } } Look for us! } ######################## } WWW: http://www.2spi.com } ######################## } ============================================ } } } } } ==============================Original Headers============================== } 12, 26 -- From cgarber-at-2spi.com Thu Dec 8 19:18:59 2005 } 12, 26 -- Received: from diskless5.axs2000.net (mail.axs2000.net } [209.120.196.43]) } 12, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } jB91IwqH016543 } 12, 26 -- for {microscopy-at-msa.microscopy.com} ; Thu, 8 Dec 2005 } 19:18:58 -0600 } 12, 26 -- Received: from Desktop ([71.225.125.97]) } 12, 26 -- (authenticated bits=0) } 12, 26 -- by diskless5.axs2000.net (8.12.11/8.12.11) with } ESMTP id jB91IqHQ021366 } 12, 26 -- for {microscopy-at-msa.microscopy.com} ; Thu, 8 Dec 2005 } 20:18:58 -0500 } 12, 26 -- X-IDV-FirstRcvd: [71.225.125.97] } 12, 26 -- X-IDV-HELO: Desktop } 12, 26 -- X-IDV-Authenticated-User: cgarber } 12, 26 -- Message-ID: {00c101c5fc5e$8a423f90$6301a8c0-at-Desktop} } 12, 26 -- From: "Garber, Charles A." {cgarber-at-2spi.com} } 12, 26 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} } 12, 26 -- Subject: Lab fees } 12, 26 -- Date: Thu, 8 Dec 2005 20:19:01 -0500 } 12, 26 -- MIME-Version: 1.0 } 12, 26 -- Content-Type: text/plain; } 12, 26 -- format=flowed; } 12, 26 -- charset="iso-8859-1"; } 12, 26 -- reply-type=original } 12, 26 -- Content-Transfer-Encoding: 7bit } 12, 26 -- X-Priority: 3 } 12, 26 -- X-MSMail-Priority: Normal } 12, 26 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2670 } 12, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2670 } ==============================End of - Headers==============================
Greg Erdos 5410 SE 185th Ave Micanopy, FL 32667
==============================Original Headers============================== 4, 22 -- From gwe-at-ufl.edu Fri Dec 9 09:29:20 2005 4, 22 -- Received: from smtp.ufl.edu (sp11en1.nerdc.ufl.edu [128.227.74.11]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9FTJ51027307 4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 09:29:19 -0600 4, 22 -- Received: from uf-af2deb9258e4.ufl.edu (adsl-152-46-164.gnv.bellsouth.net [70.152.46.164]) 4, 22 -- (authenticated bits=0) 4, 22 -- by smtp.ufl.edu (8.13.1/8.13.4/2.5.1) with ESMTP id jB9FT6Ts090664 4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 10:29:07 -0500 4, 22 -- Message-Id: {6.2.3.4.2.20051209102828.01e918f8-at-biotech.ufl.edu} 4, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 4, 22 -- Date: Fri, 09 Dec 2005 10:29:17 -0500 4, 22 -- To: microscopy-at-microscopy.com 4, 22 -- From: Greg Erdos {gwe-at-ufl.edu} 4, 22 -- Subject: Re: [Microscopy] Lab fees 4, 22 -- In-Reply-To: {200512090126.jB91QA7R017945-at-ns.microscopy.com} 4, 22 -- References: {200512090126.jB91QA7R017945-at-ns.microscopy.com} 4, 22 -- Mime-Version: 1.0 4, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 4, 22 -- X-Spam-Status: hits=0, required=5, tests= 4, 22 -- X-UFL-Spam-Status: hits=0, required=5, tests= 4, 22 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 4, 22 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
} } I would respectfully suggest that discussions about fees among those of } } us } } who privide services for a fee should not be a part of this listserver.
Many of us run facilities, other shared services or businesses. It is not collusion to discuss rate structures in public. In fact, it is required by law that we post our rates in public.
It benefits all of us, including our clients and customers, when we discuss on this listserv any issues regarding microscopy or providing microscopy services, especially if they will enhance our knowledge and ability to provide better services. This helps EVERYBODY.
As a personal aside, it offends my libertarian bent when gov't competes in the free market, so I sort of agree with the screeds, but I'm really tired of the anti-university-facility screeds periodically posted to this listserv. Furthermore, I don't know about the rest of the world, but in New York it is extremely difficult for a microscopy service to balance a budget even with subsidization and I don't think there is much of a market for microscopy outside of universities and corporations that largely have their own equipment and expertise in house. In other words, this discussion is hypotheticals and philosophy, not practical concerns.
_________________________________________ Michael Cammer Analytical Imaging Facility and Dept. ASB Biophotonics Innovation Laboratory Albert Einstein College of Medicine 1300 Morris Park Avenue, Bronx, NY 10461 718-430-2890 Fax 718-430-8996 work: http://www.aecom.yu.edu/aif/ personal: http://coxcammer.com/
==============================Original Headers============================== 12, 31 -- From cammer-at-aecom.yu.edu Fri Dec 9 10:44:59 2005 12, 31 -- Received: from mailgw.aecom.yu.edu (mailgw.aecom.yu.edu [129.98.1.16]) 12, 31 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9GixJL016490 12, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 10:44:59 -0600 12, 31 -- Received: from mailvx.aecom.yu.edu (mailvx.aecom.yu.edu [129.98.1.17]) 12, 31 -- by mailgw.aecom.yu.edu (8.12.11/8.12.11) with SMTP id jB9Gimtd007014 12, 31 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 11:44:58 -0500 12, 31 -- Received: from netmail.aecom.yu.edu ([129.98.1.112]) 12, 31 -- by mailvx.aecom.yu.edu (SAVSMTP 3.1.1.32) with SMTP id M2005120911450015564 12, 31 -- ; Fri, 09 Dec 2005 11:45:00 -0500 12, 31 -- Received: from netmail.aecom.yu.edu (netmail [129.98.1.112]) 12, 31 -- by netmail.aecom.yu.edu (Postfix) with ESMTP id C78D021BE; 12, 31 -- Fri, 9 Dec 2005 11:44:58 -0500 (EST) 12, 31 -- Received: from 162.83.204.111 12, 31 -- (SquirrelMail authenticated user cammer) 12, 31 -- by netmail.aecom.yu.edu with HTTP; 12, 31 -- Fri, 9 Dec 2005 11:44:58 -0500 (EST) 12, 31 -- Message-ID: {50020.162.83.204.111.1134146698.squirrel-at-netmail.aecom.yu.edu} 12, 31 -- In-Reply-To: {200512091557.jB9FvOjV003369-at-ns.microscopy.com} 12, 31 -- References: {200512091557.jB9FvOjV003369-at-ns.microscopy.com} 12, 31 -- Date: Fri, 9 Dec 2005 11:44:58 -0500 (EST) 12, 31 -- Subject: Re: [Microscopy] Re: Lab fees 12, 31 -- From: "Michael Cammer" {cammer-at-aecom.yu.edu} 12, 31 -- To: microscopy-at-microscopy.com 12, 31 -- User-Agent: SquirrelMail/1.4.4 12, 31 -- MIME-Version: 1.0 12, 31 -- Content-Type: text/plain;charset=iso-8859-1 12, 31 -- X-Priority: 3 (Normal) 12, 31 -- Importance: Normal 12, 31 -- Content-Transfer-Encoding: 8bit 12, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jB9GixJL016490 ==============================End of - Headers==============================
The last time I looked I could not find my copy, but NSF long ago published guidelines regarding work by federally funded labs for outside users. I also can not find it on the NSF web site (which seems to have only much newer stuff).
As I remember them, the NSF guidelines said that any instruments funded by NSF may be used to perform work for users outside the institution in which they are located only in the following circumstances: 1 If no commercial analytical laboratory has equipment that can do the job. 2 If no commercial analytical laboratory wishes to do the job. 3 If the samples would be prejudiced by traveling to a commercial analytical laboratory that is further away. 4 If the work is performed as a genuine research collaboration between the outside user and the host institution. and that even if these conditions apply, the rates charged should be comparable with those of commercial laboratories.
My belief is that DOE refers the people it funds to the NSF guidelines, so that the guidelines can be considered rather general for all federally funded equipment. The idea is that any work not covered by these guidelines is unfair competition for the commercial laboratories. Note that it is necessary (under these guidelines) to charge as much as the commercial labs but that in itself is not enough.
For twenty years I have operated under these guidelines (at Illinois and at Lehigh) and on many occasions I have sent potential users to commercial labs (and thereby turned down funds that were very much needed). Even if the NSF document has become lost, I still use the principles it establishes because they seem well founded.
Note that in Chuck Garber's email he referred only to point 4, above. There are three other reasons that make it possible to do outside work. There are some grey areas though. For example, I have allowed outside work when the outside user insisted in operating the microscope themselves and the commercial labs would not allow that. Chuck implies that the research done must be publishable. I do not recall that from the guidelines, neither does it seem logical. Universities may choose to do classified research and that should surely be included under point 4.
Still the overall principle stands. Universities should not enter into unfair competition with commercial analytical laboratories and charging more than they do is not justification enough for taking on the work
Alwyn Eades
-- ........... Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
==============================Original Headers============================== 9, 22 -- From jae5-at-lehigh.edu Fri Dec 9 12:34:02 2005 9, 22 -- Received: from rain.CC.Lehigh.EDU (rain.CC.Lehigh.EDU [128.180.39.20]) 9, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9IY1fx027719 9, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 12:34:02 -0600 9, 22 -- Received: from [128.180.55.141] (Dyn055141.mat.Lehigh.EDU [128.180.55.141]) 9, 22 -- (authenticated bits=0) 9, 22 -- by rain.CC.Lehigh.EDU (8.13.5/8.13.5) with ESMTP id jB9IY18S018283 9, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 9, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 13:34:01 -0500 9, 22 -- Message-ID: {4399CE19.2080105-at-lehigh.edu} 9, 22 -- Date: Fri, 09 Dec 2005 13:34:01 -0500 9, 22 -- From: Alwyn Eades {jae5-at-lehigh.edu} 9, 22 -- Organization: Lehigh University 9, 22 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 9, 22 -- X-Accept-Language: en-us, en 9, 22 -- MIME-Version: 1.0 9, 22 -- To: Microscopy-at-microscopy.com 9, 22 -- Subject: Lab fees and outside use of university instruments 9, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 22 -- Content-Transfer-Encoding: 7bit 9, 22 -- X-Virus-Scanned: ClamAV version 0.87.1, clamav-milter version 0.87 on rain.CC.Lehigh.EDU 9, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Without commenting on the current discussion, I would like to suggest that people identify themselves when writing to the list (full name and affiliation). A first name and an often undecipherable email address do not tell us who sent a comment or with whom we are conversing. --Jan Factor
--------------------------------------- Jan Robert Factor, Ph.D. Professor of Biology --------------------------------------- Natural Sciences Purchase College, State University of New York 735 Anderson Hill Rd. Purchase, NY 10577 USA --------------------------------------- Office Tel: 914-251-6659 Office Fax: 914-251-6635 E-mail: jfactor-at-ns.purchase.edu or- jan.factor-at-purchase.edu ---------------------------------------
DusevichV-at-umkc.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 6, 19 -- From jfactor-at-ns.purchase.edu Fri Dec 9 12:56:22 2005 6, 19 -- Received: from zephyr.ns.purchase.edu (purvid.ns.purchase.edu [199.79.168.193]) 6, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9IuLxT032065 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 9 Dec 2005 12:56:21 -0600 6, 19 -- Received: from ns.purchase.edu ([10.52.0.79]) 6, 19 -- by zephyr.ns.purchase.edu (AIX5.1/8.11.6p2/8.11.0) with ESMTP id jB9IxEJ19786; 6, 19 -- Fri, 9 Dec 2005 13:59:14 -0500 6, 19 -- Message-ID: {4399D355.3030708-at-ns.purchase.edu} 6, 19 -- Date: Fri, 09 Dec 2005 13:56:21 -0500 6, 19 -- From: Jan Factor {jfactor-at-ns.purchase.edu} 6, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.6) Gecko/20040113 6, 19 -- X-Accept-Language: en-us, en 6, 19 -- MIME-Version: 1.0 6, 19 -- To: "'Microscopy list'" {Microscopy-at-MSA.Microscopy.Com} 6, 19 -- Subject: Re: [Microscopy] RE: Re: surplus equipment for hurricane victims 6, 19 -- References: {200512091523.jB9FNSB2025709-at-ns.microscopy.com} 6, 19 -- In-Reply-To: {200512091523.jB9FNSB2025709-at-ns.microscopy.com} 6, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 6, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I would not agree with most opinions expressed here so far.
If an analytical lab in a public university does work for outside users it SHOULD charge less than the prevailing commercial fees.
If a public university is charging the prevailing commercial rates then in these rates are included the taxes and benefits a commercial organization usually charges its customers. Since the public university lab does not need to pay taxes, benefits etc. it would generate unjustifiably higher profits if it charges commercial rates and this is what I would call unfair busines practice.
Krassimir Bozhilov
______________________________________________________ Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
tel 951 927 2998 fax 951 827 2489 bozhilov-at-ucr.edu _______________________________________
==============================Original Headers============================== 8, 19 -- From bozhilov-at-ucr.edu Fri Dec 9 13:18:05 2005 8, 19 -- Received: from sentinel.ucr.edu (sentinel.ucr.edu [138.23.226.228]) 8, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9JI2U9005354 8, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 13:18:03 -0600 8, 19 -- Received: from [138.23.185.162] (igppb116g.ucr.edu [138.23.185.162]) 8, 19 -- by sentinel.ucr.edu (MOS 3.5.9-GR) 8, 19 -- with ESMTP id DNU63483 (AUTH via LOGINBEFORESMTP) 8, 19 -- for {Microscopy-at-microscopy.com} ; 8, 19 -- Fri, 9 Dec 2005 11:17:56 -0800 (PST) 8, 19 -- Mime-Version: 1.0 (Apple Message framework v746.2) 8, 19 -- Content-Transfer-Encoding: 7bit 8, 19 -- Message-Id: {295451FC-E97E-44CC-8215-BBAB5C258032-at-ucr.edu} 8, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 8, 19 -- To: Microscopy-at-microscopy.com 8, 19 -- From: KN Bozhilov {bozhilov-at-ucr.edu} 8, 19 -- Subject: [Microscopy] Lab fees and outside use of university instruments 8, 19 -- Date: Fri, 9 Dec 2005 11:17:56 -0800 8, 19 -- X-Mailer: Apple Mail (2.746.2) 8, 19 -- X-Junkmail-Status: score=0/65, host=sentinel.ucr.edu ==============================End of - Headers==============================
I tend to listen to what anyone has to say, full professor from prestigeous university or humble amateur.
Paul Barton Grover, Jr., Ph.D. Chief Microscopist, Bottle Washer, and Grand Poobah 302 Murphy St. Lafayette, IN 47905
------------------------------------------------------------ The world is so full of a number of things I'm sure we should all be as happy as kings
--Robert Louis Stevenson
-----Original Message----- X-from: jfactor-at-ns.purchase.edu [mailto:jfactor-at-ns.purchase.edu] Sent: Friday, December 09, 2005 2:04 PM To: pgrover-at-bilbo.bio.purdue.edu
Without commenting on the current discussion, I would like to suggest that people identify themselves when writing to the list (full name and affiliation). A first name and an often undecipherable email address do not tell us who sent a comment or with whom we are conversing. --Jan Factor
--------------------------------------- Jan Robert Factor, Ph.D. Professor of Biology --------------------------------------- Natural Sciences Purchase College, State University of New York 735 Anderson Hill Rd. Purchase, NY 10577 USA --------------------------------------- Office Tel: 914-251-6659 Office Fax: 914-251-6635 E-mail: jfactor-at-ns.purchase.edu or- jan.factor-at-purchase.edu ---------------------------------------
DusevichV-at-umkc.edu wrote:
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==============================Original Headers============================== 6, 19 -- From jfactor-at-ns.purchase.edu Fri Dec 9 12:56:22 2005 6, 19 -- Received: from zephyr.ns.purchase.edu (purvid.ns.purchase.edu [199.79.168.193]) 6, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9IuLxT032065 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 9 Dec 2005 12:56:21 -0600 6, 19 -- Received: from ns.purchase.edu ([10.52.0.79]) 6, 19 -- by zephyr.ns.purchase.edu (AIX5.1/8.11.6p2/8.11.0) with ESMTP id jB9IxEJ19786; 6, 19 -- Fri, 9 Dec 2005 13:59:14 -0500 6, 19 -- Message-ID: {4399D355.3030708-at-ns.purchase.edu} 6, 19 -- Date: Fri, 09 Dec 2005 13:56:21 -0500 6, 19 -- From: Jan Factor {jfactor-at-ns.purchase.edu} 6, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.6) Gecko/20040113 6, 19 -- X-Accept-Language: en-us, en 6, 19 -- MIME-Version: 1.0 6, 19 -- To: "'Microscopy list'" {Microscopy-at-MSA.Microscopy.Com} 6, 19 -- Subject: Re: [Microscopy] RE: Re: surplus equipment for hurricane victims 6, 19 -- References: {200512091523.jB9FNSB2025709-at-ns.microscopy.com} 6, 19 -- In-Reply-To: {200512091523.jB9FNSB2025709-at-ns.microscopy.com} 6, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 6, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 18, 25 -- From pgrover-at-bilbo.bio.purdue.edu Fri Dec 9 13:40:21 2005 18, 25 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 18, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9JeKjG012316 18, 25 -- for {microscopy-at-microscopy.org} ; Fri, 9 Dec 2005 13:40:20 -0600 18, 25 -- Received: from paklabpgrover (dhcp155-122.bio.purdue.edu [128.210.155.122]) 18, 25 -- by mailhub129.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id jB9JeJ7E022894 18, 25 -- for {microscopy-at-microscopy.org} ; Fri, 9 Dec 2005 14:40:19 -0500 18, 25 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu} 18, 25 -- To: {microscopy-at-microscopy.org} 18, 25 -- Subject: RE: [Microscopy] Re: surplus equipment for hurricane victims 18, 25 -- Date: Fri, 9 Dec 2005 14:40:23 -0500 18, 25 -- Message-ID: {000101c5fcf8$659e0170$7a9bd280-at-paklabpgrover} 18, 25 -- MIME-Version: 1.0 18, 25 -- Content-Type: text/plain; 18, 25 -- charset="us-ascii" 18, 25 -- X-Priority: 3 (Normal) 18, 25 -- X-MSMail-Priority: Normal 18, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.4510 18, 25 -- Importance: Normal 18, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 18, 25 -- In-Reply-To: {200512091904.jB9J4PU9001826-at-ns.microscopy.com} 18, 25 -- X-PMX-Version: 4.7.1.128075 18, 25 -- X-PerlMx-Virus-Scanned: Yes 18, 25 -- Content-Transfer-Encoding: 8bit 18, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jB9JeKjG012316 ==============================End of - Headers==============================
re: rate structure for recharge and other "off-campus" stuff
You may want to check out the following: Office of Management Budget (OMB) Circular A-21, "Cost Principles for Colleges and Universities" http://www.whitehouse.gov/omb/circulars/a021/a021.html
regards,
Jim
********************************************************** Dr. Jim Quinn james.quinn-at-stonybrook.edu Materials Science 631-632-6663 FAX:8052 Stony Brook University www.matscieng.stonybrook.edu Stony Brook, New York 11794 - 2275 **********************************************************
==============================Original Headers============================== 10, 12 -- From jquinn-at-www.matscieng.sunysb.edu Fri Dec 9 13:59:04 2005 10, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 10, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9Jx0hS018148 10, 12 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 13:59:01 -0600 10, 12 -- Received: (from jquinn-at-localhost) 10, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id jB9JwFQ09081 10, 12 -- for microscopy-at-microscopy.com; Fri, 9 Dec 2005 14:58:15 -0500 10, 12 -- Date: Fri, 9 Dec 2005 14:58:15 -0500 10, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 10, 12 -- Message-Id: {200512091958.jB9JwFQ09081-at-www.matscieng.sunysb.edu} 10, 12 -- To: microscopy-at-microscopy.com 10, 12 -- Subject: re: rate structure ==============================End of - Headers==============================
} Without commenting on the current discussion, I would like to suggest } that people identify themselves when writing to the list } (full name and } affiliation). A first name and an often undecipherable email } address do } not tell us who sent a comment or with whom we are } conversing. --Jan Factor } } --------------------------------------- } Jan Robert Factor, Ph.D.
My apologies.
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
If you were a potential user, without regard to legality nor NFS/DOE restrictions, would you choose the more expensive commercial lab or the cheaper university lab?
Alan Stone
At 01:41 PM 12/9/2005, you wrote:
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Alan Stone ASTON Metallurgical Services Co., Inc. 200 Larkin Drive Ste A Wheeling, IL 60090 847/353-8100 www.astonmet.com
==============================Original Headers============================== 13, 23 -- From as-at-astonmet.com Fri Dec 9 14:15:27 2005 13, 23 -- Received: from outbound2.mail.tds.net (outbound2.mail.tds.net [216.170.230.92]) 13, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9KFQO7024095 13, 23 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 14:15:26 -0600 13, 23 -- Received: from outaamta02.mail.tds.net (outaamta02.mail.tds.net [216.170.230.32]) 13, 23 -- by outbound2.mail.tds.net (8.13.4/8.12.2) with ESMTP id jB9KFLaN012475; 13, 23 -- Fri, 9 Dec 2005 14:15:23 -0600 (CST) 13, 23 -- Received: from AlansXPS.astonmet.com ([69.11.219.4]) 13, 23 -- by outaamta02.mail.tds.net with ESMTP 13, 23 -- id {20051209201520.NNZG23063.outaamta02.mail.tds.net-at-AlansXPS.astonmet.com} ; 13, 23 -- Fri, 9 Dec 2005 14:15:20 -0600 13, 23 -- Message-Id: {6.2.0.14.2.20051209141255.02535098-at-pop.tds.net} 13, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 13, 23 -- Date: Fri, 09 Dec 2005 14:15:20 -0600 13, 23 -- To: bozhilov-at-ucr.edu 13, 23 -- From: Alan Stone {as-at-astonmet.com} 13, 23 -- Subject: Re: [Microscopy] Lab fees and outside use of university 13, 23 -- instruments 13, 23 -- Cc: microscopy-at-microscopy.com 13, 23 -- In-Reply-To: {200512091941.jB9JfEYJ012547-at-ns.microscopy.com} 13, 23 -- References: {200512091941.jB9JfEYJ012547-at-ns.microscopy.com} 13, 23 -- Mime-Version: 1.0 13, 23 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
} Furthermore, I don't know about the rest of the world, but in } New York it is extremely difficult for a microscopy service to balance a } budget even with subsidization and I don't think there is much of a market } for microscopy outside of universities and corporations that largely have } their own equipment and expertise in house.
As the manager of a commercial laboratory offering SEM and light microscopy services, I can assure you that there is a considerable market for microscopy services outside of universities and large corporate labs. Our lab has two modern SEMs, one variable pressure and one field emission, that stay busy enough to be viable as a commercial enterprise despite (IMHO) unfair competition from federally subsidized university laboratories.
I understand the need for microscopy services at research universities and am sympathic to the challenges of maintaining instruments on a limited budget. But I agree that following the NSF guidelines is the ethical path for facilities with public funding.
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
==============================Original Headers============================== 5, 22 -- From hanke-at-mee-inc.com Fri Dec 9 15:05:31 2005 5, 22 -- Received: from mail2.atl.registeredsite.com (mail2.atl.registeredsite.com [64.224.219.76]) 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9L5UkE010559 5, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 15:05:30 -0600 5, 22 -- Received: from netmail.mail.registeredsite.com (netmail2.mail.registeredsite.com [216.122.69.15]) 5, 22 -- by mail2.atl.registeredsite.com (8.12.11/8.12.11) with ESMTP id jB9L5TBu016819 5, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 16:05:29 -0500 5, 22 -- Received: (qmail 42619 invoked by uid 89); 9 Dec 2005 21:15:19 -0000 5, 22 -- Received: from unknown (HELO ?192.168.1.109?) (216.43.123.204) 5, 22 -- by 216.122.69.20 with SMTP; 9 Dec 2005 21:15:19 -0000 5, 22 -- Message-ID: {4399F193.5000609-at-mee-inc.com} 5, 22 -- Date: Fri, 09 Dec 2005 15:05:23 -0600 5, 22 -- From: Larry Hanke {hanke-at-mee-inc.com} 5, 22 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 5, 22 -- X-Accept-Language: en-us, en 5, 22 -- MIME-Version: 1.0 5, 22 -- To: Microscopy-at-microscopy.com 5, 22 -- Subject: Re: Lab fees and outside use of university instruments 5, 22 -- References: {200512091932.jB9JWVH9009839-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {200512091932.jB9JWVH9009839-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
If you look back in time, you will see that when Nestor changed the filtering scheme on 3 July 2005, people's names were dropped from the posting header. What was left was their e-mail address... their "Reply To" address. Some of these are obvious while some are not. So I suppose for those that are not obvious what their name is, it is a good idea to put one's name in their posting. However, if you look at the bottom of each posting, the old name header info is still there. Same for you as for Vladimir.
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==============================Original Headers============================== 11, 24 -- From gary-at-gaugler.com Fri Dec 9 15:11:38 2005 11, 24 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jB9LBZb8012909 11, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 15:11:36 -0600 11, 24 -- Received: (qmail 17175 invoked from network); 9 Dec 2005 13:10:37 -0800 11, 24 -- Received: by simscan 1.1.0 ppid: 17162, pid: 17163, t: 3.1763s 11, 24 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1204 spam: 3.0.3 11, 24 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 24 -- by qsmtp3 with SMTP; 9 Dec 2005 13:10:34 -0800 11, 24 -- Message-Id: {6.2.3.4.2.20051209130306.028e6448-at-mail.calweb.com} 11, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 11, 24 -- Date: Fri, 09 Dec 2005 13:11:32 -0800 11, 24 -- To: jfactor-at-ns.purchase.edu 11, 24 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 24 -- Subject: Re: [Microscopy] Re: surplus equipment for hurricane victims 11, 24 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 24 -- In-Reply-To: {200512091923.jB9JMxdw006914-at-ns.microscopy.com} 11, 24 -- References: {200512091923.jB9JMxdw006914-at-ns.microscopy.com} 11, 24 -- Mime-Version: 1.0 11, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 11, 24 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp3.surewest.net 11, 24 -- X-Spam-Level: 11, 24 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 11, 24 -- version=3.0.3 ==============================End of - Headers==============================
My facility does pay state sales tax on all purchases and also charges state sales tax on any invoices. We also include an overhead charge for all invoices outside the university.
Even with those additional charges our cost would still be well below the cost for the same service at a corporation. The main reasons are:
The money to acquire capital equipment is from taxes in the form of grants. This is money that does not need to be recovered. Salaries at a public university tend to be somewhat less than in the corporate world. We are non-profit and only have to cover out costs.
These items give the university a totally unfair advantage over a for profit corporation. Another aspect is the unfair situation of the University using tax money, some of which is paid by the corporation it would be competing with, to undercut corporations.
Our policy is to charge a substantially larger rate for non- university work, onto which is added an overhead charge and sales tax.
The possible advantages to using this University's facility would be:
We have equipment that is not available locally. We are willing to train users and allow them to run the equipment. We have a pool of knowledge readily available.
Pricing is not an advantage, since we have to charge at least the going rate in the corporate world.
All that being said, My facility does very little work for non- university entities.
Tom
On Dec 9, 2005, at 11:33 AM, bozhilov-at-ucr.edu wrote:
} } I would not agree with most opinions expressed here so far. } } If an analytical lab in a public university does work for outside } users it SHOULD charge less than the prevailing commercial fees. } } If a public university is charging the prevailing commercial rates } then in these rates are included the taxes and benefits a commercial } organization usually charges its customers. Since the public } university lab does not need to pay taxes, benefits etc. it would } generate unjustifiably higher profits if it charges commercial rates } and this is what I would call unfair busines practice. } } Krassimir Bozhilov } } ______________________________________________________ } Central Facility for Advanced Microscopy and Microanalysis } University of California } Riverside, CA 92521 } } tel 951 927 2998 } fax 951 827 2489 } bozhilov-at-ucr.edu } _______________________________________ ------------------------------------------------------------------------ --------------------------------------------- Thomas M Murray email: murraytm-at-u.washington.edu Electron Microscopy Center Manager Phone: (206)543-2836 Materials Science & Engineering Fax: (206)543-3100 Box 352120 302 Roberts Hall Cell: (425)345-0083 University of Washington Seattle, WA 98195
==============================Original Headers============================== 14, 26 -- From murraytm-at-u.washington.edu Fri Dec 9 15:42:47 2005 14, 26 -- Received: from mxout4.cac.washington.edu (mxout4.cac.washington.edu [140.142.33.19]) 14, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9LgijU024221 14, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 15:42:45 -0600 14, 26 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.33.9]) 14, 26 -- by mxout4.cac.washington.edu (8.13.5+UW05.10/8.13.5+UW05.09) with ESMTP id jB9LghD1029536 14, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 14, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 13:42:43 -0800 14, 26 -- X-Auth-Received: from [128.95.118.89] (tstoebe-mse.ad.engr.washington.edu [128.95.118.89]) 14, 26 -- (authenticated authid=murraytm) 14, 26 -- by smtp.washington.edu (8.13.5+UW05.10/8.13.5+UW05.09) with ESMTP id jB9LggQU030216 14, 26 -- (version=TLSv1/SSLv3 cipher=RC4-SHA bits=128 verify=NOT) 14, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 13:42:43 -0800 14, 26 -- In-Reply-To: {200512091933.jB9JXXLe010156-at-ns.microscopy.com} 14, 26 -- References: {200512091933.jB9JXXLe010156-at-ns.microscopy.com} 14, 26 -- Mime-Version: 1.0 (Apple Message framework v746.2) 14, 26 -- X-Priority: 3 14, 26 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 14, 26 -- Message-Id: {7A1E0D8C-243C-4F03-8E7E-4C7614B9727B-at-u.washington.edu} 14, 26 -- Content-Transfer-Encoding: 7bit 14, 26 -- From: Tom Murray {murraytm-at-u.washington.edu} 14, 26 -- Subject: Re: [Microscopy] Lab fees and outside use of university instruments 14, 26 -- Date: Fri, 9 Dec 2005 13:42:40 -0800 14, 26 -- To: "Microscopy ListServer (E-mail)" {Microscopy-at-microscopy.com} 14, 26 -- X-Mailer: Apple Mail (2.746.2) 14, 26 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CHILD_PORN_NOT_1 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __HAS_X_PRIORITY 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_CRUFT 0' ==============================End of - Headers==============================
Paul, True, but it often helps one to understand the thrust of some of these posts if you have a better idea of who they are and where they're from.
What I find frustrating is when someone posts a need for service and all that is on the posting for identification is a name and -at-hotmail.com. Is this person down the street from me, across the country or are customs and immigration a major consideration (not to mention export restrictions on some things to some places)?
Simply having a "signature" automatically appended to your emails takes care of the problem and keeps everyone informed.
My $0.02
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: pgrover-at-bilbo.bio.purdue.edu [mailto:pgrover-at-bilbo.bio.purdue.edu] Sent: Friday, December 09, 2005 3:50 PM To: kenconverse-at-qualityimages.biz
I tend to listen to what anyone has to say, full professor from prestigeous university or humble amateur.
Paul Barton Grover, Jr., Ph.D. Chief Microscopist, Bottle Washer, and Grand Poobah 302 Murphy St. Lafayette, IN 47905
------------------------------------------------------------ The world is so full of a number of things I'm sure we should all be as happy as kings
--Robert Louis Stevenson
-----Original Message----- X-from: jfactor-at-ns.purchase.edu [mailto:jfactor-at-ns.purchase.edu] Sent: Friday, December 09, 2005 2:04 PM To: pgrover-at-bilbo.bio.purdue.edu
Without commenting on the current discussion, I would like to suggest that people identify themselves when writing to the list (full name and affiliation). A first name and an often undecipherable email address do not tell us who sent a comment or with whom we are conversing. --Jan Factor
--------------------------------------- Jan Robert Factor, Ph.D. Professor of Biology --------------------------------------- Natural Sciences Purchase College, State University of New York 735 Anderson Hill Rd. Purchase, NY 10577 USA --------------------------------------- Office Tel: 914-251-6659 Office Fax: 914-251-6635 E-mail: jfactor-at-ns.purchase.edu or- jan.factor-at-purchase.edu ---------------------------------------
DusevichV-at-umkc.edu wrote:
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==============================Original Headers============================== 6, 19 -- From jfactor-at-ns.purchase.edu Fri Dec 9 12:56:22 2005 6, 19 -- Received: from zephyr.ns.purchase.edu (purvid.ns.purchase.edu [199.79.168.193]) 6, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9IuLxT032065 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 9 Dec 2005 12:56:21 -0600 6, 19 -- Received: from ns.purchase.edu ([10.52.0.79]) 6, 19 -- by zephyr.ns.purchase.edu (AIX5.1/8.11.6p2/8.11.0) with ESMTP id jB9IxEJ19786; 6, 19 -- Fri, 9 Dec 2005 13:59:14 -0500 6, 19 -- Message-ID: {4399D355.3030708-at-ns.purchase.edu} 6, 19 -- Date: Fri, 09 Dec 2005 13:56:21 -0500 6, 19 -- From: Jan Factor {jfactor-at-ns.purchase.edu} 6, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.6) Gecko/20040113 6, 19 -- X-Accept-Language: en-us, en 6, 19 -- MIME-Version: 1.0 6, 19 -- To: "'Microscopy list'" {Microscopy-at-MSA.Microscopy.Com} 6, 19 -- Subject: Re: [Microscopy] RE: Re: surplus equipment for hurricane victims 6, 19 -- References: {200512091523.jB9FNSB2025709-at-ns.microscopy.com} 6, 19 -- In-Reply-To: {200512091523.jB9FNSB2025709-at-ns.microscopy.com} 6, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 6, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 18, 25 -- From pgrover-at-bilbo.bio.purdue.edu Fri Dec 9 13:40:21 2005 18, 25 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 18, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9JeKjG012316 18, 25 -- for {microscopy-at-microscopy.org} ; Fri, 9 Dec 2005 13:40:20 -0600 18, 25 -- Received: from paklabpgrover (dhcp155-122.bio.purdue.edu [128.210.155.122]) 18, 25 -- by mailhub129.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id jB9JeJ7E022894 18, 25 -- for {microscopy-at-microscopy.org} ; Fri, 9 Dec 2005 14:40:19 -0500 18, 25 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu} 18, 25 -- To: {microscopy-at-microscopy.org} 18, 25 -- Subject: RE: [Microscopy] Re: surplus equipment for hurricane victims 18, 25 -- Date: Fri, 9 Dec 2005 14:40:23 -0500 18, 25 -- Message-ID: {000101c5fcf8$659e0170$7a9bd280-at-paklabpgrover} 18, 25 -- MIME-Version: 1.0 18, 25 -- Content-Type: text/plain; 18, 25 -- charset="us-ascii" 18, 25 -- X-Priority: 3 (Normal) 18, 25 -- X-MSMail-Priority: Normal 18, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.4510 18, 25 -- Importance: Normal 18, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 18, 25 -- In-Reply-To: {200512091904.jB9J4PU9001826-at-ns.microscopy.com} 18, 25 -- X-PMX-Version: 4.7.1.128075 18, 25 -- X-PerlMx-Virus-Scanned: Yes 18, 25 -- Content-Transfer-Encoding: 8bit 18, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jB9JeKjG012316 ==============================End of - Headers==============================
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==============================Original Headers============================== 37, 24 -- From kenconverse-at-qualityimages.biz Fri Dec 9 17:13:03 2005 37, 24 -- Received: from qualityimages.biz (dpmail16.doteasy.com [65.61.209.16]) 37, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9ND2Fj021886 37, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 9 Dec 2005 17:13:02 -0600 37, 24 -- Received: from Ken [68.64.242.101] by qualityimages.biz with ESMTP 37, 24 -- (SMTPD32-8.05) id AF7ABDA0106; Fri, 09 Dec 2005 15:12:58 -0800 37, 24 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 37, 24 -- To: {pgrover-at-bilbo.bio.purdue.edu} , 37, 24 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 37, 24 -- Subject: RE: [Microscopy] RE: Re: surplus equipment for hurricane victims 37, 24 -- Date: Fri, 9 Dec 2005 18:12:48 -0500 37, 24 -- Message-ID: {001b01c5fd16$1736eab0$1b00000a-at-Ken} 37, 24 -- MIME-Version: 1.0 37, 24 -- Content-Type: text/plain; 37, 24 -- charset="us-ascii" 37, 24 -- X-Priority: 3 (Normal) 37, 24 -- X-MSMail-Priority: Normal 37, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 37, 24 -- x-mimeole: Produced By Microsoft MimeOLE V6.00.2900.2180 37, 24 -- Importance: Normal 37, 24 -- In-Reply-To: {200512092050.jB9Ko56s004791-at-ns.microscopy.com} 37, 24 -- X-IMSTrailer: __IMail_7__ 37, 24 -- Content-Transfer-Encoding: 8bit 37, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jB9ND2Fj021886 ==============================End of - Headers==============================
There are many issues being brought up with this seemingly simple subject. Judging by the number of times the subject gets aired, these issues never appear to be resolved.
Should universities encourage outside users? Probably not. Despite the push from administrators to "encourage" EM facilities to make money, these facilities are being massively subsidize. Subsidies come from university support for salaries, presumably in the form of student fees and other income, as well as from government grants awarded to perform specific research work.
Should all EM users pay the real costs for their work? In a university setting where the subsidies are in place in part to encourage faculty members to make use of shared facilities, the answer is "probably not". Low cost EM availability is often used as a recruiting tool by universities so it may be unreasonable for users to pay the actual costs to keep shared imaging facilities running. I am sure that all academic EM labs would soon disappear if such a charging scale was put in place.
Should EM users know what the real costs are? Absolutely. As a community we have the responsibility of knowing how much, in real terms, we are worth. Only then can we start to expect our colleagues to appreciate the costs of EM. However, government granting systems are such that there is no built in way of meeting actual costs, hence the concept of a shared facility.
Personally, I think that university imaging facilities attracting outside, commercial users is a bad thing. If there is insufficient demand for the facility within the university, then perhaps a re-evaluation for the need of such a facility should be made.
Although much blame for academic institutions poaching work from commercial laboratories can be put on administrative pressures to make money, people in academic environments should also be responsible enough to admit to some of the blame. Academic labs should be offering something that is unavailable elsewhere and is so good that there is a queue of faculty, post-docs and students waiting outside every morning. I must admit that I see little of this at the moment.
Regards,
Paul Webster
(and I agree completely that we should place an identifier at the end of each message - not to be snobbish but to understand a little extra of the message context)
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} Furthermore, I don't know about the rest of the world, but in } New York it is extremely difficult for a microscopy service to balance a } budget even with subsidization and I don't think there is much of a market } for microscopy outside of universities and corporations that largely have } their own equipment and expertise in house.
As the manager of a commercial laboratory offering SEM and light microscopy services, I can assure you that there is a considerable market for microscopy services outside of universities and large corporate labs. Our lab has two modern SEMs, one variable pressure and one field emission, that stay busy enough to be viable as a commercial enterprise despite (IMHO) unfair competition from federally subsidized university laboratories.
I understand the need for microscopy services at research universities and am sympathic to the challenges of maintaining instruments on a limited budget. But I agree that following the NSF guidelines is the ethical path for facilities with public funding.
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
==============================Original Headers============================== 19, 18 -- From PWebster-at-hei.org Fri Dec 9 17:23:49 2005 19, 18 -- Received: from hi0sml1.hei.org (House-Ear-Institute-1114861.cust-rtr.pacbell.net [71.136.31.242]) 19, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB9NNmWH024876 19, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 17:23:48 -0600 19, 18 -- Received: from 10.10.42.113 ([10.10.42.113]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 19, 18 -- Fri, 9 Dec 2005 23:22:41 +0000 19, 18 -- User-Agent: Microsoft-Entourage/11.2.1.051004 19, 18 -- Date: Fri, 09 Dec 2005 15:23:46 -0800 19, 18 -- Subject: Re: Lab fees and outside use of university instruments 19, 18 -- From: "Webster, Paul" {PWebster-at-hei.org} 19, 18 -- To: {Microscopy-at-microscopy.com} 19, 18 -- Message-ID: {BFBF5202.6C37%PWebster-at-hei.org} 19, 18 -- Thread-Topic: Lab fees and outside use of university instruments 19, 18 -- Thread-Index: AcX9F5oE2HVWpGkKEdqp1AANk7Zh7g== 19, 18 -- Mime-version: 1.0 19, 18 -- Content-type: text/plain; 19, 18 -- charset="US-ASCII" 19, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
My lab has more than it can handle serving my own institution. The rare outside contracts come through collaborations with our faculty or by word of mouth from former students or faculty who have been pleased with our work. We certainly do not solicit external users. Our administration has told us that our first obligation is to our internal users. We get requests for service from around the country and around the world. For biologists, distance makes the process extremely difficult and I always encourage folks that they find resources closer to home, be it in the private sector or at a University. They are usually persistent in wanting us to do the work, having spent some effort in an unsuccessful search for services elsewhere. The outside clients that we do serve are seeking quality service and technical expertise, but more important to them is the wide variety of scientific talent that can be tapped at a large research university. So basically we are in a different kind of business than the commercial labs.
At 05:34 PM 12/9/2005 -0600, you wrote:
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Gregory W. Erdos, Ph.D. Assistant Director, Interdisciplinary Center for Biotechnology Research Scientific Director, Electron Microscopy Core Lab P.O. Box 118525 University of Florida Gainesville, FL 32611 Phone: 352-392-1295 Fax: 352-846-0251 Email: gwe-at-ufl.edu
==============================Original Headers============================== 7, 23 -- From gwe-at-ufl.edu Fri Dec 9 18:15:07 2005 7, 23 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 7, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBA0F5Xg006514 7, 23 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2005 18:15:06 -0600 7, 23 -- Received: from uf-af2deb9258e4.ufl.edu (adsl-152-46-164.gnv.bellsouth.net [70.152.46.164]) 7, 23 -- (authenticated bits=0) 7, 23 -- by smtp.ufl.edu (8.13.4/8.13.4/2.5.1) with ESMTP id jBA0F06N2633906; 7, 23 -- Fri, 9 Dec 2005 19:15:01 -0500 7, 23 -- Message-Id: {6.2.3.4.2.20051209185832.01ea2cd8-at-biotech.ufl.edu} 7, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 7, 23 -- Date: Fri, 09 Dec 2005 19:15:13 -0500 7, 23 -- To: PWebster-at-hei.org, microscopy-at-microscopy.com 7, 23 -- From: Greg Erdos {gwe-at-ufl.edu} 7, 23 -- Subject: Re: [Microscopy] Re: Lab fees and outside use of university 7, 23 -- instruments 7, 23 -- In-Reply-To: {200512092334.jB9NYpuB028205-at-ns.microscopy.com} 7, 23 -- References: {200512092334.jB9NYpuB028205-at-ns.microscopy.com} 7, 23 -- Mime-Version: 1.0 7, 23 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 23 -- X-Spam-Status: hits=0, required=5, tests= 7, 23 -- X-UFL-Spam-Status: hits=0, required=5, tests= 7, 23 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 7, 23 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
Jim Quinn wrote: ======================================= Folks re: rate structure for recharge and other "off-campus" stuff
You may want to check out the following: Office of Management Budget (OMB) Circular A-21, "Cost Principles for Colleges and Universities" http://www.whitehouse.gov/omb/circulars/a021/a021.html ============================================== I could not find any place in this document where is addressed the issue of universities competing with for-profit tax-paying entities.
But I would suggest that anyone interested in seeing the divergence in views between what some believe to be the case vs. what is actually the case, check out URL http://prism.mit.edu/nsf.in91/in91.htm
You can see the full text of the original NSF Important Notice 91 and the updated document that replaced it, NSF Important Notice 122.
And while I realize I am taking it a bit out of context, one quote from Important Notice 91: ------------------ NSF-supported instrumentation or facilities may be used by or for the for-profit sector only when such use does not constitute provision of services equivalent to services available on a commercial basis. -------------
And a quote from the more recent and current Important Notice 122: ------------------- It is contrary to the NSF's intent for grantees to use NSF-supported research instrumentation or facilities to provide services for a fee in competition with private companies in a manner that is prohibited by OMB Circular A-110. ------------
Note that NSF is not saying it is OK to compete provided one charges a commercial rate. Using the instrumentation in competition with private firms is contrary to NSF intent. That is a line drawn in the sand. But the documents also recognize, as do commercial laboratories, that there are times, such as for perishable samples, or a unique instrumentation capability where such instrumentation use would be acceptable. But ask any representative from a commercial laboratory, the rub is not with these almost "outlier" situations, the problem is with the routine repetitive kinds of inspection and microscopy and failure analysis work that come up and get done every day.
When a university accepts an NSF grant, their administrators are required to sign off on a document certifying that they are in compliance with Important Notice 122 (and before that, Important Notice 91). I would think that enforcement people at NSF, if any are on this list, would find quite interesting how some employees of their grantee institutions interpret (and follow) published NSF guidelines.
Disclaimer: Structure Probe, Inc. is a for-profit tax-paying laboratory that experiences unfair competition from certain universities on an almost daily basis so we have a vested interest in seeing that something is done to reduce its frequency of occurrence.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
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==============================Original Headers============================== 15, 26 -- From cgarber-at-2spi.com Fri Dec 9 22:03:58 2005 15, 26 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 15, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBA43wtR021820 15, 26 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Dec 2005 22:03:58 -0600 15, 26 -- Received: from Desktop ([71.225.125.97]) 15, 26 -- (authenticated bits=0) 15, 26 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id jBA43sRN010774 15, 26 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Dec 2005 23:03:57 -0500 15, 26 -- X-IDV-FirstRcvd: [71.225.125.97] 15, 26 -- X-IDV-HELO: Desktop 15, 26 -- X-IDV-Authenticated-User: cgarber 15, 26 -- Message-ID: {0d0801c5fd3e$ae4b7e70$6301a8c0-at-Desktop} 15, 26 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 15, 26 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 15, 26 -- Subject: More on lab fees and outside users 15, 26 -- Date: Fri, 9 Dec 2005 23:03:29 -0500 15, 26 -- MIME-Version: 1.0 15, 26 -- Content-Type: text/plain; 15, 26 -- format=flowed; 15, 26 -- charset="iso-8859-1"; 15, 26 -- reply-type=original 15, 26 -- Content-Transfer-Encoding: 7bit 15, 26 -- X-Priority: 3 15, 26 -- X-MSMail-Priority: Normal 15, 26 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2670 15, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2670 ==============================End of - Headers==============================
I was going to let this go but in the spirit of fueling controversy, I congratulate you for doing something I have still not managed to do.
That is to create enough income to pay for my salary, my benefits (retirement and health), the salaries and benefits for all the people working for me, the service contracts for the machines, supplies, utility costs (electrical, water and waste) and the other related costs for the building. Put into that the costs for improving buildings, equipment inventory and repairs, and it is a truly wonderful achievement.
All replies and comments are welcomed because I probably deserve to be told to mind my own business.
Regards,
Paul Webster
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles CA 90057 (213) 273 8026.
-----Original Message----- X-from: Lou Ann Miller [mailto:lamiller-at-uiuc.edu] Sent: Fri 12/9/2005 7:15 PM To: Webster, Paul
I cannot get any response from universities or businesses to provide bio hazard human pathogen SEM specimens...dead of course. I seek specimens that are characteristic of the pathogens: bacteria, fungi, parasites, that are of interest to humans. These can be SEM specimens, STEM specimens or TEM negs. Several postings have resulted in no responses. So I guess the academic labs cannot offer anything. And I don't know where else they might be offered.
I am willing to pay for the specimens...no takers. why not? I don't know why. There are many human-related specimens that are probably used and dumped. Pity.
In this case, I think that the universities are better set up and qualified to deal with this sort of material...IMO. So how does this factor into the fee situation? No product at any price is quite a different matter.
gary g.
At 03:49 PM 12/9/2005, you wrote: } [snip] } } Although much blame for academic institutions poaching work from commercial } laboratories can be put on administrative pressures to make money, people in } academic environments should also be responsible enough to admit to some of } the blame. Academic labs should be offering something that is unavailable } elsewhere and is so good that there is a queue of faculty, post-docs and } students waiting outside every morning. I must admit that I see little of } this at the moment. } } Regards, } } Paul Webster
==============================Original Headers============================== 9, 25 -- From gary-at-gaugler.com Sat Dec 10 00:20:49 2005 9, 25 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jBA6KlpV006852 9, 25 -- for {microscopy-at-microscopy.com} ; Sat, 10 Dec 2005 00:20:47 -0600 9, 25 -- Received: (qmail 7986 invoked from network); 9 Dec 2005 22:19:22 -0800 9, 25 -- Received: by simscan 1.1.0 ppid: 7973, pid: 7974, t: 5.9773s 9, 25 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1142 spam: 3.0.3 9, 25 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 9, 25 -- by qsmtp2 with SMTP; 9 Dec 2005 22:19:16 -0800 9, 25 -- Message-Id: {6.2.3.4.2.20051209220822.028e1fc0-at-mail.calweb.com} 9, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 9, 25 -- Date: Fri, 09 Dec 2005 22:20:43 -0800 9, 25 -- To: PWebster-at-hei.org 9, 25 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 25 -- Subject: Re: [Microscopy] Re: Lab fees and outside use of university 9, 25 -- instruments 9, 25 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 25 -- In-Reply-To: {200512092349.jB9Nnw0s032317-at-ns.microscopy.com} 9, 25 -- References: {200512092349.jB9Nnw0s032317-at-ns.microscopy.com} 9, 25 -- Mime-Version: 1.0 9, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 9, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on qsmtp2.surewest.net 9, 25 -- X-Spam-Level: 9, 25 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 9, 25 -- version=3.0.3 ==============================End of - Headers==============================
FYI, here is a brief exerpt from the NSF WWW Site, which you can download as http://www.nsf.gov/pubs/gc1/gc1_605.pdf
Grant General Conditions (GC-1), June 15, 2005 Document Type: Policies and Procedures Document Number: gc1605 Document History: Posted June 10, 2005.
======================================================================== " The grantee shall not use equipment acquired with Federal funds to provide services to non-Federal outside organizations for a fee that is less than private companies charge for equivalent services, unless specifically authorized by statute in accordance with 2 CFR §215.34(b). " ========================================================================
Alternatively you can refer to: http://whitehouse.fed.us/omb/fedreg/2004/040511_grants.pdf which documents statue 2 CFR §215.34(b). which says essentially the same thing as the above NSF quotation.
Of course, the loop holes here are the phrases " equivalent services", "outside organizations" and " specifically authorized by statute".
Note this does not prohibit outside (non-Federal non-organizational) users, it just sets the boundary conditions on rates which must be charged. It also does not specify the rates that you charge internal (organizational) users.
With these points in mind, discussing rates that are charged to your institutional users and how one determines those rates is not illegal and is a valid discussion topic for this listserver. This does not violate any NSF rules that I currently know about.
Nestor Your Friendly Neighborhood SysOp
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Lab Electron Microscopy Center Materials Science Division/Bldg 212 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov =========================================== TPMLab: http://tpm.amc.anl.gov MMSite: http://www.amc.anl.gov ===========================================
The box said ... "This program requires Win 95/98/NT or better..." So I bought a Mac !
===========================================
==============================Original Headers============================== 14, 16 -- From zaluzec-at-aaem.amc.anl.gov Sat Dec 10 10:06:05 2005 14, 16 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3]) 14, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBAG64Fp001397 14, 16 -- for {microscopy-at-microscopy.com} ; Sat, 10 Dec 2005 10:06:04 -0600 14, 16 -- Received: from [146.139.72.105] (aem105.amc.anl.gov [146.139.72.105]) 14, 16 -- by aaem.amc.anl.gov (8.12.11/8.12.10) with ESMTP id jBAG64lH027708 14, 16 -- for {microscopy-at-microscopy.com} ; Sat, 10 Dec 2005 10:06:04 -0600 14, 16 -- Mime-Version: 1.0 14, 16 -- Message-Id: {p06110400bfc0a86b864c-at-[146.139.72.105]} 14, 16 -- Date: Sat, 10 Dec 2005 10:06:03 -0600 14, 16 -- To: microscopy-at-microscopy.com 14, 16 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} 14, 16 -- Subject: NSF Rules on Fees/Charges for Instrument Usage 14, 16 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 14, 16 -- Content-Transfer-Encoding: 8bit 14, 16 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jBAG64Fp001397 ==============================End of - Headers==============================
Since 1990, I have operated an independent, for-profit analytical laboratory specializing in Atomic Force Microscopy. Competition with universities offering like services at lower than commercial rates has been a serious problem for us. I would like to comment on a few of the posts in this discussion.
Jim Quinn of Stony Brook Univ. wrote:
"re: rate structure for recharge and other "off-campus" stuff You may want to check out the following:
Office of Management Budget (OMB) Circular A-21, "Cost Principles for Colleges and Universities" http://www.whitehouse.gov/omb/circulars/a021/a021.html"
--This document is very large. Can you point out the specific sections that relate to this discussion?
Alwyn Eades, Department of Materials Science and Engineering, Lehigh University wrote:
"Universities should not enter into unfair competition with commercial analytical laboratories and charging more than they do is not justification enough for taking on the work"
--I found this particularly ironic, since one of my prospective customers is sending routine AFM analyses to Lehigh University. He buys blocks of instrument time under the guise of supporting Lehigh's research as an "Industrial Affiliate."
Gregory W. Erdos, Ph.D., of Interdisciplinary Center for Biotechnology Research, University of Florida wrote:
"My lab has more than it can handle serving my own institution. The rare outside contracts come through collaborations with our faculty or by word of mouth from former students or faculty who have been pleased with our work. We certainly do not solicit external users. The outside clients that we do serve are seeking quality service and technical expertise, but more important to them is the wide variety of scientific talent that can be tapped at a large research university. So basically we are in a different kind of business than the commercial labs."
--I submit that the universities providing analytical services are in a substantially similar business as the commercial labs. The top commercial lab managers network with other commercial laboratories which offer related services. In this manner, one lab can manage a project drawing on the wide variety of scientific talent that exists nationwide and worldwide.
Although Dr. Erdos does not solicit outside work, he does some and thereby contributes to our problem. My observation of the economics of the commercial labs providing AFM services is that, as a group, we are suffering the "death of a thousand cuts". When 1000 university labs take on 1 outside job a year, this corresponds to 10 full-time analytical scientists in the commercial labs.
How does my company survive? The answer is superior quality of service. One of our overseas customers told me he had tried various university labs in his region of the United Kingdom. He found the AFM at one university was not working, at another it was not calibrated, at a third there were no unused probe tips. What we provide our customers is this: -a working, calibrated instrument
-a plentiful supply of probe tips, with much variety
-unique test methods and know-how
-experienced scientists operate the AFM and write the reports. We know what a good image looks like so that bad imaging conditions can be corrected "on the fly". We interpret the images so that the customer gets clear answers to the questions being investigated.
regards, Don Chernoff ================================== Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.]
==============================Original Headers============================== 27, 23 -- From donc-at-asmicro.com Sun Dec 11 22:36:04 2005 27, 23 -- Received: from smtp104.sbc.mail.re2.yahoo.com (smtp104.sbc.mail.re2.yahoo.com [68.142.229.101]) 27, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jBC4a4cW003298 27, 23 -- for {microscopy-at-microscopy.com} ; Sun, 11 Dec 2005 22:36:04 -0600 27, 23 -- Received: (qmail 67543 invoked from network); 12 Dec 2005 04:36:03 -0000 27, 23 -- Received: from unknown (HELO ASM11) (asmicro-at-sbcglobal.net-at-68.57.205.80 with login) 27, 23 -- by smtp104.sbc.mail.re2.yahoo.com with SMTP; 12 Dec 2005 04:36:03 -0000 27, 23 -- Message-ID: {002301c5fed5$7bb5d350$c901a8c0-at-ASM11} 27, 23 -- Reply-To: "Don Chernoff at ASM" {donc-at-asmicro.com} 27, 23 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 27, 23 -- To: {jquinn-at-www.matscieng.sunysb.edu} , 27, 23 -- "Microscopy List" {microscopy-at-microscopy.com} 27, 23 -- References: {200512092126.jB9LQ03w017866-at-ns.microscopy.com} 27, 23 -- Subject: Re: [Microscopy] rate structure 27, 23 -- Date: Sun, 11 Dec 2005 23:34:35 -0500 27, 23 -- MIME-Version: 1.0 27, 23 -- Content-Type: text/plain; 27, 23 -- charset="iso-8859-1" 27, 23 -- Content-Transfer-Encoding: 7bit 27, 23 -- X-Priority: 3 27, 23 -- X-MSMail-Priority: Normal 27, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1506 27, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1506 ==============================End of - Headers==============================
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Question: We highly apprecite if some can provide us commercial support to analyze following samples by SEM-EDS-WDS-EBSD or TEM.
1. Analyze Mn and C segregation present in steel samples. We tried to analyze the samples by SEM-EDS but unsuccessful due to poor static and low concentration difference between the matrix (0.8%) and the bands (1.2%) and bad contrast in image.
2. Analyze and quantify the phases (Ferrite, Martensite and Bainite) present in steel samples.
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==============================Original Headers============================== 8, 19 -- From eggert-at-mikroanalytik.de Mon Dec 12 09:35:11 2005 8, 19 -- Received: from Mail1.KONTENT.De (mail1.kontent.de [81.88.34.36]) 8, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBCFZADW010965 8, 19 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2005 09:35:10 -0600 8, 19 -- Received: from mikroanalytik.de (p54BDDA8A.dip.t-dialin.net [84.189.218.138]) 8, 19 -- by Mail1.KONTENT.De (Postfix) with ESMTP id 18AB410DA961; 8, 19 -- Mon, 12 Dec 2005 16:33:35 +0100 (CET) 8, 19 -- Message-ID: {439D9A2D.6090609-at-mikroanalytik.de} 8, 19 -- Date: Mon, 12 Dec 2005 16:41:33 +0100 8, 19 -- From: Frank Eggert {eggert-at-mikroanalytik.de} 8, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; de-DE; rv:1.4) Gecko/20030619 Netscape/7.1 (ax) 8, 19 -- X-Accept-Language: de, de-de, en 8, 19 -- MIME-Version: 1.0 8, 19 -- To: microscopy-at-microscopy.com, ahmadsam-at-sabic.com 8, 19 -- Subject: Re: [Microscopy] viaWWW: Locate Analysis Facility 8, 19 -- References: {200512121437.jBCEbL9n003533-at-ns.microscopy.com} 8, 19 -- In-Reply-To: {200512121437.jBCEbL9n003533-at-ns.microscopy.com} 8, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 8, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Over here at the University of California at Davis, we have our own microscopy center (SEMs, TEMs, etc). The administrator running the facility is trying to get a little more organized in terms of inventory, purchases, scheduling, etc. I'd written a note to our local computer list asking if anyone knew of a useful software package for organizing such a place...Quickbooks, for example, might do the trick. Someone mentioned there are packages geared specifically towards microscopy areas, and suggested I ask this list.
So, does anyone have any thoughts or recommendations on software to handle inventory, purchasing, users, etc? We actually have online scheduling...this would be more for the money side of things.
Thank you for your time,
Christopher Derr UC Davis
==============================Original Headers============================== 5, 20 -- From cmderr-at-ucdavis.edu Mon Dec 12 10:26:35 2005 5, 20 -- Received: from mx4.ucdavis.edu (mx4.ucdavis.edu [169.237.104.14]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBCGQYtG021902 5, 20 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2005 10:26:34 -0600 5, 20 -- Received: from [169.237.64.27] (lymond.engr.ucdavis.edu [169.237.64.27]) 5, 20 -- (authenticated bits=0) 5, 20 -- by mx4.ucdavis.edu (8.13.3/8.13.1/it-defang-5.4.0) with ESMTP id jBCGQUDt016299 5, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 5, 20 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2005 08:26:34 -0800 (PST) 5, 20 -- Message-ID: {439DA4CE.2050100-at-ucdavis.edu} 5, 20 -- Date: Mon, 12 Dec 2005 08:26:54 -0800 5, 20 -- From: Christopher Derr {cmderr-at-ucdavis.edu} 5, 20 -- User-Agent: Mozilla Thunderbird 1.0.7 (Windows/20050923) 5, 20 -- X-Accept-Language: en-us, en 5, 20 -- MIME-Version: 1.0 5, 20 -- To: microscopy-at-microscopy.com 5, 20 -- Subject: Small Business Software 5, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 20 -- Content-Transfer-Encoding: 7bit 5, 20 -- X-Scanned-By: MIMEDefang 2.49 on 169.237.104.14 ==============================End of - Headers==============================
Henrik, The image shows a problem with orthagonality. I'm assuming that the specimen is NOT tilted. If that is the case, then an oscilloscope would show that the X signal has a significant amount of Y summed to it.
I checked my schematics for a 35GF and there doesn't appear to be any correction adjustment for orthagonality. It would be located in the magnification section if present.
Do you have a scan rotation and tilt correction module? I don't seem to have a schematic for it but this module could malfunction in such a way as to give you exactly what you are seeing. It could be as simple as a dirty switch or a stuck relay.
Try turning the rotation function on and off, also try rotating the dial and see if the distortion changes. If it does, the problem is in the scan rotation, tilt correction module. If the distortion doesn't change, my best guess is that there is some cross-talk between the X and Y signals in the vicinity of the magnification section. It could be a cabling error, given the magnitude of the distortion.
Good luck.
Ken converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: Henrik.Kaker-at-guest.arnes.si [mailto:Henrik.Kaker-at-guest.arnes.si] Sent: Thursday, December 08, 2005 9:40 AM To: kenconverse-at-qualityimages.biz
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Email: Henrik.Kaker-at-guest.arnes.si Name: Henrik Kaker
Organization: SEM-EDS Lab
Title-Subject: [Filtered] Image distortion in Jeol JSM 35-CF
Question: Good Morning, All
In our old SEM we found image distortion. Please see the image of Cu grid at http://www.kaker.com/300x.jpg. Can anyone help me to correct this error in our SEM. Any assistance available is greatly appreciated.
==============================Original Headers============================== 9, 12 -- From zaluzec-at-microscopy.com Thu Dec 8 08:10:10 2005 9, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 9, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jB8EA8XL023126 9, 12 -- for {microscopy-at-microscopy.com} ; Thu, 8 Dec 2005 08:10:09 -0600 9, 12 -- Mime-Version: 1.0 9, 12 -- X-Sender: (Unverified) 9, 12 -- Message-Id: {p06110402bfbdef1e0d76-at-[206.69.208.22]} 9, 12 -- Date: Thu, 8 Dec 2005 08:10:06 -0600 9, 12 -- To: microscopy-at-microscopy.com 9, 12 -- From: Henrik.Kaker-at-guest.arnes.si (by way of MicroscopyListserver) 9, 12 -- Subject: viaWWW: Image distortion in Jeol JSM 35-CF 9, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
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==============================Original Headers============================== 30, 24 -- From kenconverse-at-qualityimages.biz Mon Dec 12 11:14:48 2005 30, 24 -- Received: from qualityimages.biz (dpmail16.doteasy.com [65.61.209.16]) 30, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBCHEkBe001240 30, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 12 Dec 2005 11:14:47 -0600 30, 24 -- Received: from Ken [68.64.242.101] by qualityimages.biz with ESMTP 30, 24 -- (SMTPD32-8.05) id AFF71C3E00A8; Mon, 12 Dec 2005 09:14:31 -0800 30, 24 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 30, 24 -- To: {Henrik.Kaker-at-guest.arnes.si} , 30, 24 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 30, 24 -- Subject: RE: [Microscopy] viaWWW: Image distortion in Jeol JSM 35-CF 30, 24 -- Date: Mon, 12 Dec 2005 12:14:19 -0500 30, 24 -- Message-ID: {001601c5ff3f$84224c70$6501a8c0-at-Ken} 30, 24 -- MIME-Version: 1.0 30, 24 -- Content-Type: text/plain; 30, 24 -- charset="us-ascii" 30, 24 -- X-Priority: 3 (Normal) 30, 24 -- X-MSMail-Priority: Normal 30, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 30, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 30, 24 -- Importance: Normal 30, 24 -- In-Reply-To: {200512081440.jB8EeBti029116-at-ns.microscopy.com} 30, 24 -- X-IMSTrailer: __IMail_7__ 30, 24 -- Content-Transfer-Encoding: 8bit 30, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jBCHEkBe001240 ==============================End of - Headers==============================
We've recently upgraded our JEOL 5600 computer and software, now using v. 5.26 of the GUI. Since it no longer seems fashionable to talk about "bugs" in software, I'd be interested in contacting someone with this version of software to see if the "feature" I have occurs on every machine, or just a peculiarity of my upgrade. This involves instruments that have the PRD (photo recording device) installed, or at least with that option enabled in software. Please contact me off-list.
Thanks in advance,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University Sackville, NB E4L 1G7 CANADA
Does anyone have tips for preparing cross-sectional TEM samples of multi-layers on MgO? These are 1" single crystal wafers. I need to use a non-FIB method like dimpling or tripod polishing.
Thanks, Leslie
Leslie Krupp (Thompson) IBM Almaden Research 650 Harry Road, K19/D2 San Jose, CA 95120-6099
==============================Original Headers============================== 6, 26 -- From lkrupp-at-us.ibm.com Mon Dec 12 12:50:02 2005 6, 26 -- Received: from e5.ny.us.ibm.com (e5.ny.us.ibm.com [32.97.182.145]) 6, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBCIo1Wh013083 6, 26 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2005 12:50:02 -0600 6, 26 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236]) 6, 26 -- by e5.ny.us.ibm.com (8.12.11/8.12.11) with ESMTP id jBCInwRZ018650 6, 26 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2005 13:49:58 -0500 6, 26 -- Received: from d01av04.pok.ibm.com (d01av04.pok.ibm.com [9.56.224.64]) 6, 26 -- by d01relay04.pok.ibm.com (8.12.10/NCO/VERS6.8) with ESMTP id jBCInxHD115666 6, 26 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2005 13:49:59 -0500 6, 26 -- Received: from d01av04.pok.ibm.com (loopback [127.0.0.1]) 6, 26 -- by d01av04.pok.ibm.com (8.12.11/8.13.3) with ESMTP id jBCInwVG001994 6, 26 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2005 13:49:58 -0500 6, 26 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) 6, 26 -- by d01av04.pok.ibm.com (8.12.11/8.12.11) with ESMTP id jBCInw1U001988 6, 26 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2005 13:49:58 -0500 6, 26 -- Subject: TEM cross-sections of MgO substrates 6, 26 -- To: microscopy-at-microscopy.com 6, 26 -- X-Mailer: Lotus Notes Release 6.0 September 26, 2002 6, 26 -- Message-ID: {OF96CE800B.EC603043-ON852570D5.0066D91A-882570D5.0067530A-at-us.ibm.com} 6, 26 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} 6, 26 -- Date: Mon, 12 Dec 2005 10:49:57 -0800 6, 26 -- X-MIMETrack: Serialize by Router on D01ML604/01/M/IBM(Release 7.0HF90 | November 16, 2005) at 6, 26 -- 12/12/2005 13:49:58 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
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Question: Hi there, I am looking for information about magnetic etching and I found in this web site a quite old message about it. The thing is that I cannot access to the Metals Handbook is recommended there. I would like to use Ferrofluids EMG 708 and 308 for visualizing the martensite phase transformed in a 304LN at the tip of a fatigue crack. My questions are: -The sample is electropolished before the fatigue test, do I have to chemically etch the surface around the crack before applying the Ferrofluids? -How big should be the applied magnetic field to magnetize the martensite before applying the Ferrofluids? -I have read in a paper I have to dilute the Ferrofluids in water and to add a wetting agent. What is this wetting agent? Which is its function? Thank you very much, Sonia
I apologize if this has been send before, but I just heard of Stan Erlandsen's death on Monday December 5th 2005. An MSA supporter and past-president, Stan will be remembered by many in the microscopy community. Here is the HCS web page with more details:
-- John Mansfield PhD CPhys CSci MInstP North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 (Leaving a phone message at 936-3352 is preferable to 834-3913) Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: Lat. 42° 16' 48" Long. 83° 43' 48" AIM: thejfmjfm Yahoo: thejfmjfm Skype: thejfmjfm
==============================Original Headers============================== 6, 18 -- From jfmjfm-at-umich.edu Tue Dec 13 10:09:57 2005 6, 18 -- Received: from smtp.engin.umich.edu (smtp.engin.umich.edu [141.213.75.24]) 6, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBDG9uLW001427 6, 18 -- for {microscopy-at-microscopy.com} ; Tue, 13 Dec 2005 10:09:57 -0600 6, 18 -- Received: from [141.213.21.60] (jfm-mac.engin.umich.edu [141.213.21.60]) 6, 18 -- (authenticated bits=0) 6, 18 -- by smtp.engin.umich.edu (8.12.11/8.12.10) with ESMTP id jBDG9u0g014508 6, 18 -- for {microscopy-at-microscopy.com} ; Tue, 13 Dec 2005 11:09:56 -0500 (EST) 6, 18 -- Mime-Version: 1.0 (Apple Message framework v746.2) 6, 18 -- Message-Id: {5F01C654-BB9A-4D45-8468-ACA0F15D85CB-at-umich.edu} 6, 18 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 6, 18 -- To: microscopy-at-microscopy.com 6, 18 -- From: John Mansfield {jfmjfm-at-umich.edu} 6, 18 -- Subject: Sad News - Stanley L. Erlandsen 6, 18 -- Date: Tue, 13 Dec 2005 11:09:36 -0500 6, 18 -- X-Mailer: Apple Mail (2.746.2) 6, 18 -- Content-Transfer-Encoding: 8bit 6, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jBDG9uLW001427 ==============================End of - Headers==============================
Sonia Mato asked about the use of ferrofluid to visualize magnetic phases. She asked 3 questions. My responses are inserted after each one. My questions are: -The sample is electropolished before the fatigue test, do I have to chemically etch the surface around the crack before applying the Ferrofluids? --I think this depends on what resolution you want to achieve when you image the surface. I assume that the martensite phase is produced by the fatigue process, not the chemical etch. Therefore, I suggest try first without etching. -How big should be the applied magnetic field to magnetize the martensite before applying the Ferrofluids? --try a strong disc-shaped permanent magnet, like the rare earth magnets sold by Edmund Scientific. Try to hold it about 1-2 mm above the sample surface. -I have read in a paper I have to dilute the Ferrofluids in water and to add a wetting agent. What is this wetting agent? Which is its function? --In our lab we use "Joy" brand liquid dish detergent as the wetting agent and dilute at least 10x. The purpose is to keep the ferrofluid particles from clumping together. The particle size might be 50 nm or smaller, which allows high resolution imaging of magnetic domains by optical microscope and SEM. Magnetic domains can be imaged with or without ferrofluid particles using AFM in Magnetic Force Microscopy mode. I hope this helps. regards, Don Chernoff ================================== Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.]
----- Original Message ----- From: sonia.mato-at-upc.edu To: donc-at-asmicro.com Sent: Monday, December 12, 2005 4:55 PM Subject: [a] [Microscopy] viaWWW: magnetic etching
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Question: Hi there, I am looking for information about magnetic etching and I found in this web site a quite old message about it. The thing is that I cannot access to the Metals Handbook is recommended there. I would like to use Ferrofluids EMG 708 and 308 for visualizing the martensite phase transformed in a 304LN at the tip of a fatigue crack. My questions are: -The sample is electropolished before the fatigue test, do I have to chemically etch the surface around the crack before applying the Ferrofluids? -How big should be the applied magnetic field to magnetize the martensite before applying the Ferrofluids? -I have read in a paper I have to dilute the Ferrofluids in water and to add a wetting agent. What is this wetting agent? Which is its function? Thank you very much, Sonia
O.k., here's a shot in the dark. On eof the labs here has been using Ralph knives with a rotary microtome. (Ralph Knives: The glass knives broken with a 25mm long edge rather than the 6-9mm thickness). But now they are trying to cut 1-3um sections, and they cut fine but the block face scrapes the backside of the knive on the return stroke. So looking for solutions:
(1) Ultramicrotomes with their "d" motion (backup before return stroke) work ideally but they do not hold knives large enough for the desired sections (i.e. ralph knives). Does any one know of a method of using ralph knives in an ultramicrotome?
(2) It seems once apon a time there was a rotary microtome which pulled the samples back before the return stroke. Does anyone out there happen to have such a beast sitting around gathering dust they would consider oarting with?
(3) Does anyone have any other suggestions?
Thanks!
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"WE ARE MICROSOFT. RESISTANCE IS FUTILE. YOU WILL BE ASSIMILATED."
==============================Original Headers============================== 9, 23 -- From edelmare-at-muohio.edu Tue Dec 13 16:18:04 2005 9, 23 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBDMI4mA021536 9, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 13 Dec 2005 16:18:04 -0600 9, 23 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 9, 23 -- by mulnx11.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id jBDMI2u7015219 9, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 13 Dec 2005 17:18:02 -0500 9, 23 -- Received: from emf03 ([134.53.14.97]) 9, 23 -- by mulnx24.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id jBDMI29p025463 9, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 13 Dec 2005 17:18:02 -0500 9, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 9, 23 -- To: microscopy-at-Microscopy.com 9, 23 -- Date: Tue, 13 Dec 2005 17:19:41 -0500 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-type: text/plain; charset=US-ASCII 9, 23 -- Content-transfer-encoding: 7BIT 9, 23 -- Subject: Ralph knives . . . 9, 23 -- Message-ID: {439F02AD.29038.19CB052-at-localhost} 9, 23 -- Priority: normal 9, 23 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 9, 23 -- X-Real-ConnectIP: 134.53.14.97 9, 23 -- X-Scanned-By: MIMEDefang 2.45 9, 23 -- X-Scanned-By: MIMEDefang 2.52 on 134.53.6.11 ==============================End of - Headers==============================
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Richard -
We had our shop machine a more-or-less "T" shaped aluminum block that clamped in the knife holder of an ancient MT-2. It projected slightly over the front of the knife holder. We warmed it on a hot plate & added the Ralph with a bit of dental wax. Worked fine. Design one!
Caroline -- Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html
==============================Original Headers============================== 4, 17 -- From schooley-at-mcn.org Tue Dec 13 17:39:10 2005 4, 17 -- Received: from dns3.mcn.org (dns3.mcn.org [216.150.240.32]) 4, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBDNd91F003460 4, 17 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 13 Dec 2005 17:39:09 -0600 4, 17 -- Received: from [66.42.18.105] (helo=[10.0.1.2]) 4, 17 -- by dns3.mcn.org with esmtpa (Exim 4.43) 4, 17 -- id IRGN16-0008KF-IN; Tue, 13 Dec 2005 15:39:07 -0800 4, 17 -- Mime-Version: 1.0 4, 17 -- Message-Id: {a06200701bfc508de4024-at-[10.0.1.2]} 4, 17 -- In-Reply-To: {200512132313.jBDND6F2002519-at-ns.microscopy.com} 4, 17 -- References: {200512132313.jBDND6F2002519-at-ns.microscopy.com} 4, 17 -- Date: Tue, 13 Dec 2005 15:34:52 -0800 4, 17 -- To: edelmare-at-muohio.edu 4, 17 -- From: Caroline Schooley {schooley-at-mcn.org} 4, 17 -- Subject: Re: [Microscopy] Ralph knives . . . 4, 17 -- Cc: Microscopy-at-MSA.Microscopy.Com 4, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Hi listers, I have a contamination issue which I'm thinking of tackling using replication. I know the principles, roughly. There used to be plenty of expertise here when SEMs and AFMs weren't invented and we used tape and TEM to look at surface structure (I still have some lovely images from the 1960's) but it's no surprise that the people with the knowledge are long gone. So. I still have a packet of replicating tape, the Edwards 12E6 coating unit and a TEM. I have surfaces contaminated with particles a few nm in size - my hope is that they will come off in the replicating tape and be transferred to the carbon film for some nice clean EDX analysis without any ion milling or other specimen prep artefacts. Can anyone give me some advice on how to use the replicating tape? (By the way it is probably about 15 years old, will it still be any good?) And hints on getting the carbon film onto a grid would be good too, I know this is one of those techniques where little tricks make a big difference.
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==============================Original Headers============================== 7, 28 -- From richard.beanland-at-bookham.com Wed Dec 14 04:42:48 2005 7, 28 -- Received: from mail72.messagelabs.com (mail72.messagelabs.com [193.109.255.147]) 7, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jBEAglqS021420 7, 28 -- for {microscopy-at-microscopy.com} ; Wed, 14 Dec 2005 04:42:48 -0600 7, 28 -- X-VirusChecked: Checked 7, 28 -- X-Env-Sender: richard.beanland-at-bookham.com 7, 28 -- X-Msg-Ref: server-14.tower-72.messagelabs.com!1134556966!32343935!1 7, 28 -- X-StarScan-Version: 5.5.9.1; banners=bookham.com,-,- 7, 28 -- X-Originating-IP: [213.249.209.179] 7, 28 -- Received: (qmail 1415 invoked from network); 14 Dec 2005 10:42:46 -0000 7, 28 -- Received: from unknown (HELO cas-smx-brdg-01.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 7, 28 -- by server-14.tower-72.messagelabs.com with SMTP; 14 Dec 2005 10:42:46 -0000 7, 28 -- Content-class: urn:content-classes:message 7, 28 -- MIME-Version: 1.0 7, 28 -- Content-Type: text/plain; 7, 28 -- charset="iso-8859-1" 7, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 7, 28 -- Subject: RE: TEM - replicating tape 7, 28 -- Date: Wed, 14 Dec 2005 10:42:46 -0000 7, 28 -- Message-ID: {FF719652ABB4414D86916ACAAE5BBB24015FBEF0-at-cas-smx-01.caswell1.europe.bkhm.net} 7, 28 -- X-MS-Has-Attach: 7, 28 -- X-MS-TNEF-Correlator: 7, 28 -- Thread-Topic: TEM - replicating tape 7, 28 -- Thread-Index: AcYAmuLa8ncHMxNATPOd4gnXlH2NGQAABv8Q 7, 28 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 7, 28 -- To: {microscopy-at-microscopy.com} 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jBEAglqS021420 ==============================End of - Headers==============================
Thank you Caroline. I was wondering if something along these lines would work - nice to know it can work before getting one made.
On 13 Dec 2005, at 15:34, Caroline Schooley wrote:
} } --------------------------------------------------------------------- } } ------- The Microscopy ListServer -- CoSponsor: The Microscopy } } Society of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver On-Line Help } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------- } } ------- } } } } O.k., here's a shot in the dark. On eof the labs here has been } } using Ralph knives with a rotary microtome. (Ralph Knives: The glass } } knives broken with a 25mm long edge rather than the 6-9mm thickness). } } But now they are trying to cut 1-3um sections, and they cut fine but } } the block face scrapes the backside of the knive on the return } } stroke. So looking for solutions: } } } } (1) Ultramicrotomes with their "d" motion (backup before return } } stroke) work ideally but they do not hold knives large enough for the } } desired sections (i.e. ralph knives). Does any one know of a method } } of using ralph knives in an ultramicrotome? } } } } (2) It seems once apon a time there was a rotary microtome which } } pulled the samples back before the return stroke. Does anyone out } } there happen to have such a beast sitting around gathering dust they } } would consider oarting with? } } } } (3) Does anyone have any other suggestions? } } } } Richard E. Edelmann, Ph.D. } } Richard - } } We had our shop machine a more-or-less "T" shaped aluminum block that } clamped in the knife holder of an ancient MT-2. It projected slightly } over the front of the knife holder. We warmed it on a hot plate & } added the Ralph with a bit of dental wax. Worked fine. Design one! } } Caroline } -- } Caroline Schooley } Project MICRO Coordinator } Microscopy Society of America } Box 117, 45301 Caspar Point Road } Caspar, CA 95420 } Phone/FAX (707)964-9460 } Project MICRO: http://www.microscopy.org/ProjectMICRO } Intertidal invertebrates: } http://www.fortbragg.k12.ca.us/AG/marinelab.html
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"WE ARE MICROSOFT. RESISTANCE IS FUTILE. YOU WILL BE ASSIMILATED."
==============================Original Headers============================== 8, 26 -- From edelmare-at-muohio.edu Wed Dec 14 07:30:17 2005 8, 26 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 8, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBEDUHoT032556 8, 26 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 14 Dec 2005 07:30:17 -0600 8, 26 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 8, 26 -- by mulnx12.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id jBEDUEWe004380; 8, 26 -- Wed, 14 Dec 2005 08:30:15 -0500 8, 26 -- Received: from emf03 ([134.53.14.97]) 8, 26 -- by mulnx23.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id jBEDUE8Y010781; 8, 26 -- Wed, 14 Dec 2005 08:30:14 -0500 8, 26 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 8, 26 -- To: Caroline Schooley {schooley-at-mcn.org} 8, 26 -- Date: Wed, 14 Dec 2005 08:31:56 -0500 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-type: text/plain; charset=US-ASCII 8, 26 -- Content-transfer-encoding: 7BIT 8, 26 -- Subject: Re: [Microscopy] Ralph knives . . . 8, 26 -- CC: Microscopy-at-MSA.Microscopy.Com 8, 26 -- Message-ID: {439FD87C.427.4DFD012-at-localhost} 8, 26 -- Priority: normal 8, 26 -- In-reply-to: {a06200701bfc508de4024-at-[10.0.1.2]} 8, 26 -- References: {200512132313.jBDND6F2002519-at-ns.microscopy.com} 8, 26 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 8, 26 -- X-Real-ConnectIP: 134.53.14.97 8, 26 -- X-Scanned-By: MIMEDefang 2.45 8, 26 -- X-Scanned-By: MIMEDefang 2.52 on 134.53.6.10 ==============================End of - Headers==============================
Dear Richard, You do not mention what the surface is that you are removing the particles from. We have had some success here removing tiny precipitates from steel by lightly etching the steel, carbon coating it directly, scoring the carbon coat, etching the steel again and then placing it in distilled water. The carbon squares float up to be caught on a plain grid and examined in the TEM. The precipitates are free of the steel matrix for EDX and diffraction. The replicating tape was used for SEM, but should work the same way. Soften the tape in acetone and put some acetone on the surface to be replicated. Place the tape on the surface and wait about half an hour for the tape to harden up again. Place the tape, surface-of-interest-side up and carbon coat. I have not tried releasing the carbon film from replicating tape, but whatever solvent you used to soften it should dissolve it and release the carbon film. Good luck. Regards, Mary Mager Electron Microscopist Department of Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA Tel: 604-822-5648 Fax: 604-822-3619 e-mail: mager-at-interchange.ubc.ca ----- Original Message ----- X-from: {richard.beanland-at-bookham.com} To: {mager-at-interchange.ubc.ca} Sent: Wednesday, December 14, 2005 3:26 AM
Light Microscopy Technologist
There is a regular, full-time position available for a Light Microscopy Technologist available in the Light/Confocal Microscopy Service at The Jackson Laboratory in Bar Harbor, ME. Primary duties include direct operation of the various microscope systems, cameras and computer workstations including upright, inverted, confocal, laser capture microdissection and spectral karyotyping systems; providing training for customers in the use of the microscopy equipment; assisting with image capture and analysis; preparing samples for imaging (i.e. immunofluorescent labeling, spectral karyotyping, laser capture microdissection); laboratory maintenance and administrative duties (i.e. ordering supplies, monthly billing).
The position requires a BS in a biological field, preferably with a strong background in genetics and molecular biology with a good working knowledge of microscopy principles and system operations specifically for light, fluorescent and confocal microscopy as well as a strong background in immunohistochemical techniques. A strong proficiency in current computer applications is required, including operational experience with both Macintosh and PCs along with familiarity with standard imaging programs such as Adobe Photoshop and Metamorph. The applicant should have good written and verbal communication skills as well as the ability to work both independently and as part of a team supporting a variety of users in a multi-user core facility. Interested candidates should apply on-line at www.jax.org and refer to job requisition # 0337.
Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322
==============================Original Headers============================== 8, 23 -- From lesley.bechtold-at-jax.org Thu Dec 15 07:03:22 2005 8, 23 -- Received: from carmen.jax.org (carmen.jax.org [192.43.249.16]) 8, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBFD3L9M006562 8, 23 -- for {microscopy-at-microscopy.com} ; Thu, 15 Dec 2005 07:03:21 -0600 8, 23 -- Received: from jcs-mid-prod.jax.org (jcs-mid-prod.jax.org [192.43.249.134]) 8, 23 -- by carmen.jax.org (8.12.11/8.12.11) with ESMTP id jBFCxHnZ011512; 8, 23 -- Thu, 15 Dec 2005 08:03:18 -0500 (EST) 8, 23 -- (envelope-from lesley.bechtold-at-jax.org) 8, 23 -- Received: from spikey.jax.org by jcs-mid-prod.jax.org 8, 23 -- with ESMTP id 54474141134651777; Thu, 15 Dec 2005 08:02:57 -0500 8, 23 -- From: "Lesley Bechtold" {lesley.bechtold-at-jax.org} 8, 23 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 8, 23 -- "Confocal 8, 23 -- Network" {listserv-at-listserv.buffalo.edu} 8, 23 -- Subject: Position for Light/Confocal Microscopist at The Jackson Laboratory 8, 23 -- Date: Thu, 15 Dec 2005 08:02:57 -0500 8, 23 -- Message-ID: {20051215080257128.00000003236-at-spikey} 8, 23 -- X-Mailer: Oracle Connector for Outlook 10.1.1.0.5 71011 (11.0.6568) 8, 23 -- X-Accept-Language: en-us, en 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; charset=us-ascii 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jBFD3L9M006562 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fvillalovoz-at-deltacollege.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: fvillalovoz-at-deltacollege.edu Name: Frank Villalovoz
Question: Electron Microscopy adjunct instructor, one to two courses. Contact Frank Villalovoz at San Joaquin Delta College, Stockton, California Telephone (209) 954-5249 or fvillalovoz-at-deltacollege.edu to obtain more information.
I was just shown a Motic brand inverted microscope, and wondered whether anyone on the list has experience with the brand. My first impression is good, especially on price. The optics seem nice, and construction sound. Our application would be mainly in undergraduate microbiology instruction, but also for some research.
Are there any opinions out there? Feel free to reply on or off list.
Karl Hagglund Biological Sciences, SC-102B Northern Kentucky University, Nunn Drive Highland Heights, KY 41099 859-572-5238 http://semlab.nku.edu
==============================Original Headers============================== 5, 23 -- From hagglundk1-at-nku.edu Thu Dec 15 10:51:10 2005 5, 23 -- Received: from mailfe2.nku.edu (exchange.nku.edu [192.122.237.68]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBFGpAPx028651 5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 15 Dec 2005 10:51:10 -0600 5, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211); 5, 23 -- Thu, 15 Dec 2005 11:51:08 -0500 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 5, 23 -- Content-class: urn:content-classes:message 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="US-ASCII" 5, 23 -- Subject: LM MOTIC inverted scope? 5, 23 -- Date: Thu, 15 Dec 2005 11:51:09 -0500 5, 23 -- Message-ID: {F01E4499C4EC5842A8AB7198141AC72F3585C8-at-mailfac1.hh.nku.edu} 5, 23 -- X-MS-Has-Attach: 5, 23 -- X-MS-TNEF-Correlator: 5, 23 -- Thread-Topic: LM MOTIC inverted scope? 5, 23 -- Thread-Index: AcYBl7+uTS2NjIArTaGsSOndhO0D4g== 5, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu} 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- X-OriginalArrivalTime: 15 Dec 2005 16:51:08.0652 (UTC) FILETIME=[BF3922C0:01C60197] 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jBFGpAPx028651 ==============================End of - Headers==============================
I have to mount SWCNT on formvar grids for TEM examination, I have small amounts (about 0.1g) in dry form. Any suggestions for mounting would be most appreciated. If you could reply off list that would be great.
Thanks in advance,
Mike Ganger Cornell Medical College
==============================Original Headers============================== 6, 20 -- From mganger-at-optonline.net Thu Dec 15 11:19:58 2005 6, 20 -- Received: from mta5.srv.hcvlny.cv.net (mta5.srv.hcvlny.cv.net [167.206.4.200]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBFHJtIl001791 6, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 15 Dec 2005 11:19:57 -0600 6, 20 -- Received: from [127.0.0.1] (ool-43511f6b.dyn.optonline.net [67.81.31.107]) 6, 20 -- by mta5.srv.hcvlny.cv.net 6, 20 -- (Sun Java System Messaging Server 6.2-4.03 (built Sep 22 2005)) 6, 20 -- with ESMTP id {0IRJ00I8PUT42CF1-at-mta5.srv.hcvlny.cv.net} for 6, 20 -- Microscopy-at-microscopy.com; Thu, 15 Dec 2005 12:19:54 -0500 (EST) 6, 20 -- Date: Thu, 15 Dec 2005 12:19:51 -0500 6, 20 -- From: Michael Ganger {mganger-at-optonline.net} 6, 20 -- Subject: Mounting Carbon Nanotubes 6, 20 -- To: Microscopy List Server {Microscopy-at-microscopy.com} 6, 20 -- Message-id: {43A1A5B7.80006-at-optonline.net} 6, 20 -- MIME-version: 1.0 6, 20 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-transfer-encoding: 7BIT 6, 20 -- X-Accept-Language: en-us, en 6, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 6, 20 -- Gecko/20040804 Netscape/7.2 (ax) ==============================End of - Headers==============================
I was pointed to the NIST site for some info and don't seem to see it pop out at me. Does anyone have some pointers for performing residual stress analysis with TSL EBSD? I'd like to do similar reports to those of XRD. Pseudo color plots would be ideal along with quant data.
TIA, gary g.
==============================Original Headers============================== 4, 22 -- From gary-at-gaugler.com Thu Dec 15 16:25:00 2005 4, 22 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jBFMOuZl019810 4, 22 -- for {microscopy-at-microscopy.com} ; Thu, 15 Dec 2005 16:24:58 -0600 4, 22 -- Received: (qmail 5034 invoked from network); 15 Dec 2005 14:23:40 -0800 4, 22 -- Received: by simscan 1.1.0 ppid: 5027, pid: 5028, t: 0.6785s 4, 22 -- scanners: regex: 1.1.0 attach: 1.1.0 clamav: 0.84/m:34/d:1095 spam: 3.0.3 4, 22 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 4, 22 -- by qsmtp1 with SMTP; 15 Dec 2005 14:23:39 -0800 4, 22 -- Message-Id: {6.2.3.4.2.20051215142235.028ca8f0-at-mail.calweb.com} 4, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 4, 22 -- Date: Thu, 15 Dec 2005 14:24:50 -0800 4, 22 -- To: MSA listserver {microscopy-at-microscopy.com} 4, 22 -- From: Gary Gaugler {gary-at-gaugler.com} 4, 22 -- Subject: Residual Stress with EBSD 4, 22 -- Mime-Version: 1.0 4, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 4, 22 -- X-Spam-Checker-Version: SpamAssassin 3.0.3 (2005-04-27) on 4, 22 -- qsmtp1.mc.surewest.net 4, 22 -- X-Spam-Level: 4, 22 -- X-Spam-Status: No, score=-2.5 required=5.0 tests=AWL,BAYES_00 autolearn=ham 4, 22 -- version=3.0.3 ==============================End of - Headers==============================
I would consider the following web page for some information: {http://www.boulder.nist.gov/div853/Program1_Reliability_Dimensionally_Constrained.htm}
Hope this helps (and Happy Holidays)
At 05:33 PM 12/15/2005, gary-at-gaugler.com wrote:
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==============================Original Headers============================== 8, 20 -- From bonevich-at-nist.gov Fri Dec 16 08:46:53 2005 8, 20 -- Received: from smtp.nist.gov (rimp2.nist.gov [129.6.16.227]) 8, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBGEkqGN010566 8, 20 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2005 08:46:52 -0600 8, 20 -- Received: from postmark.nist.gov (pushme.nist.gov [129.6.16.92]) 8, 20 -- by smtp.nist.gov (8.13.1/8.13.1) with ESMTP id jBGEkooi026256 8, 20 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2005 09:46:50 -0500 8, 20 -- Received: from h180168.nist.gov (h180168.nist.gov [129.6.180.168]) 8, 20 -- by postmark.nist.gov (8.12.5/8.12.5) with ESMTP id jBGEjw3v026760 8, 20 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2005 09:45:58 -0500 (EST) 8, 20 -- Message-Id: {6.2.1.2.2.20051216094533.0395fd80-at-email.nist.gov} 8, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2 8, 20 -- Date: Fri, 16 Dec 2005 09:45:46 -0500 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- From: bonevich {bonevich-at-nist.gov} 8, 20 -- Subject: Re: [Microscopy] Residual Stress with EBSD 8, 20 -- Mime-Version: 1.0 8, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 20 -- X-NIST-MailScanner: Found to be clean 8, 20 -- X-NIST-MailScanner-From: bonevich-at-nist.gov ==============================End of - Headers==============================
Our new Electron Microscopy facility will be hosting two TEM's (Jeol 2200FS, Hitachi HF-3300), two SEM's (Hitachi S4800, S3000N) and maybe a dual-column FIB. There is also the possibility we will be expanding in the future.
We are looking for software that allows users to save data from any of the machines to a local server and be able to retrieve the images from their own computers (secure online storage). The ideal system would have search capabilities and would be able to display image previews.
So far, the only suitable software we found is "Quartz PCI" and we want to analyze all other options.
Thank you again for all your help. This list rocks!
Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC W6-017A ECERF Bldg, 9107-116 Street Edmonton, AB. T6G 2V4
Our folks save images locally and then we post them at an FTP site in a folder with their name. They can just bring up the site in their web browser and drag the images to their desktop and save them on their own site. Some folks want the stuff password protected, but most people do not care. We leave the images up for one month and then archive to DVD. So theoretically both the lab and the user have copies. We used to maintain our own FTP server, but now we use a departmental server that has a lot more capacity and is maintained by our IT people. Others send images directly to their own FTP site and take us out of the loop. In that case we do not archive and they are fully responsible for their data. And yet others will write a DVD or CD before they leave the lab and take images with them.
At 11:07 AM 12/16/2005 -0600, you wrote:
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Greg Erdos 5410 SE 185th Ave Micanopy, FL 32667
==============================Original Headers============================== 7, 22 -- From gwe-at-ufl.edu Fri Dec 16 11:21:11 2005 7, 22 -- Received: from smtp.ufl.edu (smtp02.osg.ufl.edu [128.227.74.165]) 7, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBGHLAbQ025499 7, 22 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2005 11:21:10 -0600 7, 22 -- Received: from uf-af2deb9258e4.ufl.edu (adsl-152-46-164.gnv.bellsouth.net [70.152.46.164]) 7, 22 -- (authenticated bits=0) 7, 22 -- by smtp.ufl.edu (8.13.4/8.13.4/2.5.1) with ESMTP id jBGHL7i51896652; 7, 22 -- Fri, 16 Dec 2005 12:21:08 -0500 7, 22 -- Message-Id: {6.2.3.4.2.20051216121447.01e87690-at-biotech.ufl.edu} 7, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 7, 22 -- Date: Fri, 16 Dec 2005 12:21:23 -0500 7, 22 -- To: Daniel.Salamon-at-nrc-cnrc.gc.ca, microscopy-at-microscopy.com 7, 22 -- From: Greg Erdos {gwe-at-ufl.edu} 7, 22 -- Subject: Re: [Microscopy] online image server 7, 22 -- In-Reply-To: {200512161707.jBGH7QrJ022741-at-ns.microscopy.com} 7, 22 -- References: {200512161707.jBGH7QrJ022741-at-ns.microscopy.com} 7, 22 -- Mime-Version: 1.0 7, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 22 -- X-Spam-Status: hits=-1.428, required=5, tests=BAYES_20 7, 22 -- X-UFL-Spam-Status: hits=-1.428, required=5, tests=BAYES_20 7, 22 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 7, 22 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
On Dec 16, 2005, at 9:01 AM, Daniel.Salamon-at-nrc-cnrc.gc.ca wrote:
} We are looking for software that allows users to save data from any of } the machines to a local server and be able to retrieve the images from } their own computers (secure online storage). The ideal system would } have } search capabilities and would be able to display image previews. } Dear Daniel, Leginon is capable of this and a lot more. Bridget Carragher and Clint Potter distribute the program and run workshops at The Scripps Research Institute. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Fri Dec 16 11:46:06 2005 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBGHk5uc032536 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 16 Dec 2005 11:46:05 -0600 4, 22 -- Received: from localhost (fire-dog [192.168.1.4]) 4, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id 84EAA3538C 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 16 Dec 2005 09:46:04 -0800 (PST) 4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 4, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id D39C133C38 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 16 Dec 2005 09:46:01 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v623) 4, 22 -- In-Reply-To: {200512161701.jBGH18Cp021545-at-ns.microscopy.com} 4, 22 -- References: {200512161701.jBGH18Cp021545-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {46bbd92793a473abd6ac32e6828f2476-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] online image server 4, 22 -- Date: Fri, 16 Dec 2005 09:51:49 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.623) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.3.2 ==============================End of - Headers==============================
---Disclaimer: I represent a commercial interest with this post.----
Soft Imaging System offers this functionality with our software. We have iTEM for TEM applications, Scandium for SEM applications, and software for LM applications as well. All versions of the software are compatible with each other and can write to a central, secure database.
In an effort to keep this from being too commercial, I will respond with further information off list.
Regards, Jason Wickersham
Jason Wickersham Sales Engineer 84 E Grand Avenue Montvale, NJ 07645 Jason.Wickersham-at-Soft-Imaging.net 551-804-1845
-----Original Message----- X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca [mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca] Sent: Friday, December 16, 2005 12:50 PM To: Jason Wickersham
Hi everyone,
Our new Electron Microscopy facility will be hosting two TEM's (Jeol 2200FS, Hitachi HF-3300), two SEM's (Hitachi S4800, S3000N) and maybe a dual-column FIB. There is also the possibility we will be expanding in the future.
We are looking for software that allows users to save data from any of the machines to a local server and be able to retrieve the images from their own computers (secure online storage). The ideal system would have search capabilities and would be able to display image previews.
So far, the only suitable software we found is "Quartz PCI" and we want to analyze all other options.
Thank you again for all your help. This list rocks!
Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC W6-017A ECERF Bldg, 9107-116 Street Edmonton, AB. T6G 2V4
I am a bit confused on whether the Ge EDS detector can have the warming up cycle or not. Our detector is dead after one warming up. What is the mechanism of the failure if it warms up? I was never told that the detector should never be warmed up by the manufacturer technical support. Is it a common knowledge that Ge detector should NEVER be warmed up once it is cold?
Thanks
Yan Xin, Ph.D National High Magnetic Field Laboratory Tallahassee, FL 32310
==============================Original Headers============================== 5, 22 -- From xin-at-magnet.fsu.edu Fri Dec 16 13:38:26 2005 5, 22 -- Received: from mail.magnet.fsu.edu (mail.magnet.fsu.edu [146.201.250.9]) 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBGJcP4h026535 5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2005 13:38:26 -0600 5, 22 -- Received: from xinlt.magnet.fsu.edu (a2-dhcp90.magnet.fsu.edu [146.201.233.90]) 5, 22 -- (authenticated bits=0) 5, 22 -- by mail.magnet.fsu.edu (8.13.1/8.13.1) with ESMTP id jBGJcKgX024543 5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2005 14:38:20 -0500 5, 22 -- Message-Id: {6.2.1.2.2.20051216141609.01dd3e38-at-mail.magnet.fsu.edu} 5, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2 5, 22 -- Date: Fri, 16 Dec 2005 14:37:18 -0500 5, 22 -- To: microscopy-at-microscopy.com 5, 22 -- From: Yan Xin {xin-at-magnet.fsu.edu} 5, 22 -- Subject: problem with PGT pure Ge EDS detector 5, 22 -- Mime-Version: 1.0 5, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 5, 22 -- X-NHMFL-MailScanner: Found to be clean 5, 22 -- X-MailScanner-MCPCheck: MCP-Clean, MCP-Checker (score=0, required 1) 5, 22 -- X-NHMFL-MailScanner-SpamCheck: not spam, SpamAssassin (score=-5.748, 5, 22 -- required 5, autolearn=not spam, ALL_TRUSTED -3.30, AWL 0.15, 5, 22 -- BAYES_00 -2.60) 5, 22 -- X-MailScanner-From: xin-at-magnet.fsu.edu ==============================End of - Headers==============================
Is it dead, or is it just the vacuum? I had a PGT Si(li) detector that PGT told me could safely be warmed up for the Christmas holidays, so, after switching off the HV, I did so. However, on re-cooling a couple of weeks later, the vacuum had drastically deteriorated to the extent that the detector had to be re-pumped. It may be that some detectors, or some manufacturer's detectors, are more likely to be damaged by warming, regardless of the manufacturer's claims or advice.
cheers
rtch
Quoting xin-at-magnet.fsu.edu:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, } } I am a bit confused on whether the Ge EDS detector can have the warming up } cycle or not. Our detector is dead after one warming up. What is the } mechanism of the failure if it warms up? I was never told that the } detector should never be warmed up by the manufacturer technical } support. Is it a common knowledge that Ge detector should NEVER be } warmed up once it is cold? } } Thanks } } Yan Xin, Ph.D } National High Magnetic Field Laboratory } Tallahassee, FL 32310 } } } ==============================Original Headers============================== } 5, 22 -- From xin-at-magnet.fsu.edu Fri Dec 16 13:38:26 2005 } 5, 22 -- Received: from mail.magnet.fsu.edu (mail.magnet.fsu.edu } [146.201.250.9]) } 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBGJcP4h026535 } 5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2005 13:38:26 -0600 } 5, 22 -- Received: from xinlt.magnet.fsu.edu (a2-dhcp90.magnet.fsu.edu } [146.201.233.90]) } 5, 22 -- (authenticated bits=0) } 5, 22 -- by mail.magnet.fsu.edu (8.13.1/8.13.1) with ESMTP id jBGJcKgX024543 } 5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2005 14:38:20 -0500 } 5, 22 -- Message-Id: {6.2.1.2.2.20051216141609.01dd3e38-at-mail.magnet.fsu.edu} } 5, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2 } 5, 22 -- Date: Fri, 16 Dec 2005 14:37:18 -0500 } 5, 22 -- To: microscopy-at-microscopy.com } 5, 22 -- From: Yan Xin {xin-at-magnet.fsu.edu} } 5, 22 -- Subject: problem with PGT pure Ge EDS detector } 5, 22 -- Mime-Version: 1.0 } 5, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } 5, 22 -- X-NHMFL-MailScanner: Found to be clean } 5, 22 -- X-MailScanner-MCPCheck: MCP-Clean, MCP-Checker (score=0, required 1) } 5, 22 -- X-NHMFL-MailScanner-SpamCheck: not spam, SpamAssassin (score=-5.748, } 5, 22 -- required 5, autolearn=not spam, ALL_TRUSTED -3.30, AWL 0.15, } 5, 22 -- BAYES_00 -2.60) } 5, 22 -- X-MailScanner-From: xin-at-magnet.fsu.edu } ==============================End of - Headers============================== }
------------------------------------------------- This mail sent through University of Auckland http://www.auckland.ac.nz/
==============================Original Headers============================== 15, 35 -- From r.sims-at-auckland.ac.nz Fri Dec 16 14:02:10 2005 15, 35 -- Received: from chico.itss.auckland.ac.nz (mailhost.auckland.ac.nz [130.216.190.12]) 15, 35 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBGK28mT032032 15, 35 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2005 14:02:09 -0600 15, 35 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 15, 35 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id 0A5353560A; 15, 35 -- Sat, 17 Dec 2005 09:02:08 +1300 (NZDT) 15, 35 -- Received: from chico.itss.auckland.ac.nz ([127.0.0.1]) 15, 35 -- by localhost (smtpb.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 15, 35 -- with ESMTP id 03694-10; Sat, 17 Dec 2005 09:02:07 +1300 (NZDT) 15, 35 -- Received: from motoko.itss.auckland.ac.nz (motoko.itss.auckland.ac.nz [130.216.191.146]) 15, 35 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id A4D6F3480F; 15, 35 -- Sat, 17 Dec 2005 09:02:07 +1300 (NZDT) 15, 35 -- Received: (from apache-at-localhost) 15, 35 -- by motoko.itss.auckland.ac.nz (8.11.6/8.11.6) id jBGK26424010; 15, 35 -- Sat, 17 Dec 2005 09:02:06 +1300 15, 35 -- Received: from 202-180-110-48.jetbuster.co.nz 15, 35 -- (202-180-110-48.jetbuster.co.nz [202.180.110.48]) by webmail.auckland.ac.nz 15, 35 -- (Horde) with HTTP for {rsim007-at-webmail.auckland.ac.nz} ; Sat, 17 Dec 2005 15, 35 -- 09:02:06 +1300 15, 35 -- Message-ID: {1134763326.d3e086a5a6ea1-at-webmail.auckland.ac.nz} 15, 35 -- Date: Sat, 17 Dec 2005 09:02:06 +1300 15, 35 -- From: r.sims-at-auckland.ac.nz 15, 35 -- To: xin-at-magnet.fsu.edu 15, 35 -- Cc: microscopy-at-microscopy.com 15, 35 -- Subject: Re: [Microscopy] problem with PGT pure Ge EDS detector 15, 35 -- References: {200512161950.jBGJoBnr029404-at-ns.microscopy.com} 15, 35 -- In-Reply-To: {200512161950.jBGJoBnr029404-at-ns.microscopy.com} 15, 35 -- MIME-Version: 1.0 15, 35 -- Content-Type: text/plain; charset="ISO-8859-1" 15, 35 -- Content-Disposition: inline 15, 35 -- Content-Transfer-Encoding: 7bit 15, 35 -- User-Agent: Internet Messaging Program (IMP) 4.0-cvs 15, 35 -- X-Originating-IP: 202.180.110.48 15, 35 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
I am in search of a solution to replace the anti-roll plate for my Leitz Kryostat 1720 cryostat. There is no source for new ones, does anyone have a solution? source? Know a guy? etc.
thanks
==============================Original Headers============================== 6, 22 -- From kayton-at-ohsu.edu Fri Dec 16 15:44:56 2005 6, 22 -- Received: from twmms01.ohsu.edu (twmms01.ohsu.edu [137.53.207.89]) 6, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBGLitwX014769 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2005 15:44:55 -0600 6, 22 -- Received: from 137.53.4.234 by twmms01.ohsu.edu with ESMTP (Tumbleweed 6, 22 -- MMS SMTP Relay (MMS v5.6.3)); Fri, 16 Dec 2005 13:44:46 -0800 6, 22 -- X-Server-Uuid: E8EE9E4E-CE80-4A3D-84F1-1599A05BA687 6, 22 -- Received: from [137.53.83.30] (udp01522846uds.ohsu.edu [137.53.83.30]) 6, 22 -- by ngw34.ohsu.edu; Fri, 16 Dec 2005 13:45:08 -0800 6, 22 -- MIME-Version: 1.0 6, 22 -- Message-ID: {23B167A2-F182-4063-A1D1-3A95AFE2D167-at-ohsu.edu} 6, 22 -- To: "Electron Microscopy Society" {microscopy-at-microscopy.com} 6, 22 -- From: "Robert Kayton" {kayton-at-ohsu.edu} 6, 22 -- Subject: Anti-Roll Plate 6, 22 -- Date: Fri, 16 Dec 2005 13:42:13 -0800 6, 22 -- X-Mailer: Apple Mail (2.734) 6, 22 -- X-WSS-ID: 6FBDEAC42BS3018807-01-01 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Content-Type: text/plain; 6, 22 -- charset=us-ascii; 6, 22 -- delsp=yes; 6, 22 -- format=flowed ==============================End of - Headers==============================
It will be interesting to hear what the manufacturers have to say officially about this. Meanwhile, I can share our story.
We had a Ge detector on an Oxford EDS system. It had served us well for years, but it appears that increasing LN2 consumption got ahead of us on one long interval between fills and the detector warmed up - at least some. We got it cooled down again and tried it out. There were peaks where they were supposed to be, but there was also a lot of intensity tailing off to the low energy side. I suppose that the crystal broke during warm up and we were having difficulty collecting all the energy from the x-rays and it was a function of the relation between where they arrived and the fracture(s) in the crystal. We had to trash that crystal and replace it.
Bottom line - I would not try warming up any detector without the express guarantee of the vendor that it won't be damaged in the process.
Warren Straszheim Iowa State University
-----Original Message----- X-from: xin-at-magnet.fsu.edu [mailto:xin-at-magnet.fsu.edu] Sent: Friday, December 16, 2005 1:48 PM To: wesaia-at-iastate.edu
Hi, Jim,
HT/HV is working, but it does seem the vacuum isn't as good as before. Usually the TEM column vacuum is below 1x10-4 after boiling off LN2 using ACD, but now it is 2x10-4. I don't know whether it is due to the detector.
The detector behaves like this with all the right HT, vacuum, beam current. When I start to collect spectrum by pressing the start button on the imix, it immediately gives 100% deadtime. Maybe I should check the counts.
Regards Yan Xin
At 04:39 PM 12/16/2005, you wrote: } Xin Yan } } I doubt that one warming cycle would } intentionally kill a EDS detector, } whether it is SiLi, GeLi, or another. } } It was probably a fluke. } } Even if the HV/HT was on, the } detector would not fail. Instead, } it would be inaccurate, once recooled. } } } Did you check that the HT/HV is working? } } Have you check the counts on an oscope? } } How about the vacuum in the dewar? } } regards, } } Jim } } } } From mail-at-ns.microscopy.com Fri Dec 16 14:46:46 2005 } } Date: Fri, 16 Dec 2005 13:47:47 -0600 } } To: jquinn-at-www.matscieng.sunysb.edu } } From: xin-at-magnet.fsu.edu } } Reply-to: xin-at-magnet.fsu.edu } } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } } Subject: [Microscopy] problem with PGT pure Ge EDS detector } } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } } X-lewp: MicroscopyListSpam NAGS } } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Hi, } } } } I am a bit confused on whether the Ge EDS detector can have the } warming up } } cycle or not. Our detector is dead after one warming up. What is the } } mechanism of the failure if it warms up? I was never told that the } } detector should never be warmed up by the manufacturer technical } } support. Is it a common knowledge that Ge detector should NEVER be } } warmed up once it is cold? } } } } Thanks } } } } Yan Xin, Ph.D } } National High Magnetic Field Laboratory } } Tallahassee, FL 32310 } } } } } } ==============================Original } Headers============================== } } 5, 22 -- From xin-at-magnet.fsu.edu Fri Dec 16 13:38:26 2005 } } 5, 22 -- Received: from mail.magnet.fsu.edu (mail.magnet.fsu.edu } [146.201.250.9]) } } 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } jBGJcP4h026535 } } 5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2005 } 13:38:26 -0600 } } 5, 22 -- Received: from xinlt.magnet.fsu.edu (a2-dhcp90.magnet.fsu.edu } [146.201.233.90]) } } 5, 22 -- (authenticated bits=0) } } 5, 22 -- by mail.magnet.fsu.edu (8.13.1/8.13.1) with ESMTP id } jBGJcKgX024543 } } 5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2005 } 14:38:20 -0500 } } 5, 22 -- Message-Id: } {6.2.1.2.2.20051216141609.01dd3e38-at-mail.magnet.fsu.edu} } } 5, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2 } } 5, 22 -- Date: Fri, 16 Dec 2005 14:37:18 -0500 } } 5, 22 -- To: microscopy-at-microscopy.com } } 5, 22 -- From: Yan Xin {xin-at-magnet.fsu.edu} } } 5, 22 -- Subject: problem with PGT pure Ge EDS detector } } 5, 22 -- Mime-Version: 1.0 } } 5, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } } 5, 22 -- X-NHMFL-MailScanner: Found to be clean } } 5, 22 -- X-MailScanner-MCPCheck: MCP-Clean, MCP-Checker (score=0, } required 1) } } 5, 22 -- X-NHMFL-MailScanner-SpamCheck: not spam, SpamAssassin } (score=-5.748, } } 5, 22 -- required 5, autolearn=not spam, ALL_TRUSTED -3.30, AWL 0.15, } } 5, 22 -- BAYES_00 -2.60) } } 5, 22 -- X-MailScanner-From: xin-at-magnet.fsu.edu } } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 6, 24 -- From xin-at-magnet.fsu.edu Fri Dec 16 16:09:54 2005 6, 24 -- Received: from mail.magnet.fsu.edu (mail.magnet.fsu.edu [146.201.250.9]) 6, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBGM9qLc020600 6, 24 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2005 16:09:53 -0600 6, 24 -- Received: from xinlt.magnet.fsu.edu (a2-dhcp90.magnet.fsu.edu [146.201.233.90]) 6, 24 -- (authenticated bits=0) 6, 24 -- by mail.magnet.fsu.edu (8.13.1/8.13.1) with ESMTP id jBGM9mjE016159; 6, 24 -- Fri, 16 Dec 2005 17:09:48 -0500 6, 24 -- Message-Id: {6.2.1.2.2.20051216164156.01e4a890-at-mail.magnet.fsu.edu} 6, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2 6, 24 -- Date: Fri, 16 Dec 2005 17:09:39 -0500 6, 24 -- To: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} , microscopy-at-microscopy.com 6, 24 -- From: Yan Xin {xin-at-magnet.fsu.edu} 6, 24 -- Subject: Re: [Microscopy] problem with PGT pure Ge EDS detector 6, 24 -- In-Reply-To: {200512162139.jBGLdnE31922-at-www.matscieng.sunysb.edu} 6, 24 -- References: {200512162139.jBGLdnE31922-at-www.matscieng.sunysb.edu} 6, 24 -- Mime-Version: 1.0 6, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 24 -- X-NHMFL-MailScanner: Found to be clean 6, 24 -- X-MailScanner-MCPCheck: MCP-Clean, MCP-Checker (score=0, required 1) 6, 24 -- X-NHMFL-MailScanner-SpamCheck: not spam, SpamAssassin (score=-5.749, 6, 24 -- required 5, autolearn=not spam, ALL_TRUSTED -3.30, AWL 0.15, 6, 24 -- BAYES_00 -2.60) 6, 24 -- X-MailScanner-From: xin-at-magnet.fsu.edu ==============================End of - Headers==============================
xin-at-magnet.fsu.edu wrote: } } } The detector behaves like this with all the right HT, vacuum, beam current. } When I start to collect spectrum by pressing the start button on the imix, } it immediately gives 100% deadtime.
You don't by any chance have an IR camera on in the chamber, do you? Detectors have no sense of humor about infrared.
Rick Mott
==============================Original Headers============================== 2, 19 -- From rickmott-at-alumni.princeton.edu Fri Dec 16 16:49:15 2005 2, 19 -- Received: from n016.sc0.cp.net (smtpout1089.sc0.cp.net [64.97.144.89]) 2, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBGMnDRC000577 2, 19 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2005 16:49:15 -0600 2, 19 -- Received: from alumni.princeton.edu (24.225.218.94) by n016.sc0.cp.net (7.2.069.1) (authenticated as rickmott-at-alumni.princeton.edu) 2, 19 -- id 438B8B5D0027018A for microscopy-at-microscopy.com; Fri, 16 Dec 2005 22:49:09 +0000 2, 19 -- Sender: rick-at-ns.microscopy.com 2, 19 -- Message-ID: {43A3444C.3E347525-at-alumni.princeton.edu} 2, 19 -- Date: Fri, 16 Dec 2005 17:48:44 -0500 2, 19 -- From: Rick Mott {rickmott-at-alumni.princeton.edu} 2, 19 -- Organization: Acuity Engineering 2, 19 -- X-Mailer: Mozilla 4.78 [en] (X11; U; Linux 2.4.7-10 i686) 2, 19 -- X-Accept-Language: en 2, 19 -- MIME-Version: 1.0 2, 19 -- To: microscopy-at-microscopy.com 2, 19 -- Subject: Re: [Microscopy] Re: problem with PGT pure Ge EDS detector 2, 19 -- References: {200512162228.jBGMScPf026441-at-ns.microscopy.com} 2, 19 -- Content-Type: text/plain; charset=us-ascii 2, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
As a general comment, I have had Si detectors warm up now and then, never during actual use though, as I always check LN levels before turning it on. I then re-cool the detector and don't turn the power on until the next day, just to be safe, and get normal functioning back.
More pertinent to your observation below of 100% dead time, one time after a warm-up and subsequent cooling down, I too saw the high deadtime, due to very high noise count, as it turned out. Of course, I "flipped out" thinking the detector was damaged! But eventually I realized that the calibration of the energy axis had "slipped" to the very low energy side of the axis and I was picking up very high noise counts from that region of the energy axis of 1 to about 0.4 KeV. This very low energy region of the system is usually blocked out by the baseline setting for normal functioning. After new calibration of energy axis using copper (just nicking the edge of a copper grid with the e-beam to get Cu-L xrays at low energy end and Cu-K xrays at high energy end), using my system's calibration program, the noise went away and normal functioning was restored.
So I suggest trying to recalibrate your energy axis. Maybe that will work for you too.
Good luck!
Gib Ahlstrand Imaging Center University of Minnesota St. Paul, MN
xin-at-magnet.fsu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, Jim, } } HT/HV is working, but it does seem the vacuum isn't as good as } before. Usually the TEM column vacuum is below 1x10-4 after boiling off } LN2 using ACD, but now it is 2x10-4. I don't know whether it is due to the } detector. } } The detector behaves like this with all the right HT, vacuum, beam current. } When I start to collect spectrum by pressing the start button on the imix, } it immediately gives 100% deadtime. } Maybe I should check the counts. } } Regards } Yan Xin
==============================Original Headers============================== 9, 20 -- From ahlst007-at-umn.edu Fri Dec 16 17:50:58 2005 9, 20 -- Received: from mtaout-c.tc.umn.edu (mtaout-c.tc.umn.edu [160.94.128.21]) 9, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBGNov5K018424 9, 20 -- for {Microscopy-at-Microscopy.com} ; Fri, 16 Dec 2005 17:50:57 -0600 9, 20 -- Received: from [160.94.39.28] (x160-94-39-28.cbs.umn.edu [160.94.39.28]) by mtaout-c.tc.umn.edu with ESMTP for Microscopy-at-Microscopy.com; Fri, 16 Dec 2005 17:50:57 -0600 (CST) 9, 20 -- X-Umn-Remote-Mta: [N] x160-94-39-28.cbs.umn.edu [160.94.39.28] #+LO+TS+AU+HN 9, 20 -- Message-ID: {43A352E1.1030707-at-umn.edu} 9, 20 -- Date: Fri, 16 Dec 2005 17:50:57 -0600 9, 20 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 9, 20 -- Reply-To: ahlst007-at-umn.edu 9, 20 -- Organization: Imaging Center UM 9, 20 -- User-Agent: Mozilla Thunderbird 1.0.7 (Macintosh/20050923) 9, 20 -- X-Accept-Language: en-us, en 9, 20 -- MIME-Version: 1.0 9, 20 -- To: Microscopy-at-Microscopy.com 9, 20 -- Subject: Re: [Microscopy] Re: problem with PGT pure Ge EDS detector 9, 20 -- References: {200512162315.jBGNFGES009279-at-ns.microscopy.com} 9, 20 -- In-Reply-To: {200512162315.jBGNFGES009279-at-ns.microscopy.com} 9, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Does anyone know how to make a filter that will eject all wavelengths of light from IR through UV, except for the hydrogen alpha band?
Mel Pollinger New York Microscopical Society
==============================Original Headers============================== 3, 13 -- From zaluzec-at-microscopy.com Mon Dec 19 08:03:16 2005 3, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 3, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBJE3Gtc016990 3, 13 -- for {microscopy-at-microscopy.com} ; Mon, 19 Dec 2005 08:03:16 -0600 3, 13 -- Mime-Version: 1.0 3, 13 -- X-Sender: (Unverified) 3, 13 -- Message-Id: {p06110400bfcc6dd5ebfd-at-[206.69.208.22]} 3, 13 -- Date: Mon, 19 Dec 2005 08:03:15 -0600 3, 13 -- To: microscopy-at-microscopy.com 3, 13 -- From: "MEL POLLINGER" {pollingmel-at-verizon.net} (by way of 3, 13 -- MicroscopyListserver) 3, 13 -- Subject: viaWWW: Microscope as telescope Hydrogen Alpha filter 3, 13 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dwaugh-at-kent.edu) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, December 18, 2005 at 20:52:20 ---------------------------------------------------------------------------
Email: dwaugh-at-kent.edu Name: David Waugh
Organization: Kent State University
Education: Graduate College
Location: Kent Ohio
Question: SEM sample replication
First I want to thank all the people who have helped me in the past, I have not always thanked them individually via email, but people on this list have been exceedingly helpful.
I am beginning a project that will involve the replication of fossil and rock surfaces for examination under the SEM (gold coated, in most cases well under 1000X). I was going to try and use a replication compound made by Struers (Repliset) and a dental casting compound Examix NDS (Hydrophilic Vinyl Polysiloxane). For making positive replicas I was going to try Epothin epoxy and SPI positive replica powder (polyethylene homopolymer), and the casting compounds themselves. Does anyone have experience using any of these compounds or know of better alternatives? The casts need to be flexible so that they can be removed from undercuts. The epoxy I have read about is Araldite, I could not find out what model number they used, or if it is some kind of special epoxy? Thanks, Have a good holiday! -David
David A. Waugh Kent State University Department of Geology Kent, Ohio 44242 dwaugh-at-kent.edu Http://www.personal.kent.edu/~dwaugh/
Do you need to make one? Why not just buy a H-alpha filter from one of the ads in "Astronomy" or "Sky and Telescope"? Or, a store that sells telescopes to amateur astronomers. They're relatively cheap, and can have sharp cut-offs.
Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
-----Original Message----- X-from: pollingmel-at-verizon.net [mailto:pollingmel-at-verizon.net] Sent: Mon 05/12/19 10:11 To: Oshel, Philip Eugene
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Does anyone know how to make a filter that will eject all wavelengths of light from IR through UV, except for the hydrogen alpha band?
Mel Pollinger New York Microscopical Society
==============================Original Headers============================== 3, 13 -- From zaluzec-at-microscopy.com Mon Dec 19 08:03:16 2005 3, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 3, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBJE3Gtc016990 3, 13 -- for {microscopy-at-microscopy.com} ; Mon, 19 Dec 2005 08:03:16 -0600 3, 13 -- Mime-Version: 1.0 3, 13 -- X-Sender: (Unverified) 3, 13 -- Message-Id: {p06110400bfcc6dd5ebfd-at-[206.69.208.22]} 3, 13 -- Date: Mon, 19 Dec 2005 08:03:15 -0600 3, 13 -- To: microscopy-at-microscopy.com 3, 13 -- From: "MEL POLLINGER" {pollingmel-at-verizon.net} (by way of 3, 13 -- MicroscopyListserver) 3, 13 -- Subject: viaWWW: Microscope as telescope Hydrogen Alpha filter 3, 13 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
==============================Original Headers============================== 15, 30 -- From oshel1pe-at-cmich.edu Mon Dec 19 09:14:47 2005 15, 30 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 15, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBJFEkE3002577 15, 30 -- for {microscopy-at-microscopy.com} ; Mon, 19 Dec 2005 09:14:46 -0600 15, 30 -- Received: from egateb.central.cmich.local ([141.209.15.85]) 15, 30 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id jBJFuA4l029784 15, 30 -- for {microscopy-at-microscopy.com} ; Mon, 19 Dec 2005 10:56:10 -0500 15, 30 -- Received: from cmail4.central.cmich.local ([141.209.15.84]) by egateb.central.cmich.local with Microsoft SMTPSVC(5.0.2195.6713); 15, 30 -- Mon, 19 Dec 2005 10:14:31 -0500 15, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 15, 30 -- Content-class: urn:content-classes:message 15, 30 -- MIME-Version: 1.0 15, 30 -- Content-Type: text/plain; 15, 30 -- charset="iso-8859-1" 15, 30 -- Subject: RE: [Microscopy] viaWWW: Microscope as telescope Hydrogen Alpha filter 15, 30 -- Date: Mon, 19 Dec 2005 10:11:59 -0500 15, 30 -- Message-ID: {07BD45BB3EE8A0468C94776E7A0C660E3A3192-at-cmail4.central.cmich.local} 15, 30 -- X-MS-Has-Attach: 15, 30 -- X-MS-TNEF-Correlator: 15, 30 -- Thread-Topic: [Microscopy] viaWWW: Microscope as telescope Hydrogen Alpha filter 15, 30 -- Thread-Index: AcYErn8Y+49RitsySl+kaumd/OCO2wAAA+IU 15, 30 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 15, 30 -- To: {pollingmel-at-verizon.net} 15, 30 -- Cc: {microscopy-at-microscopy.com} 15, 30 -- X-OriginalArrivalTime: 19 Dec 2005 15:14:31.0968 (UTC) FILETIME=[E9C85A00:01C604AE] 15, 30 -- X-CanItPRO-Stream: default 15, 30 -- X-Spam-Score: -4 () L_EXCH_MF 15, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 15, 30 -- Content-Transfer-Encoding: 8bit 15, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jBJFEkE3002577 ==============================End of - Headers==============================
I have used both the Repliset and dental casting coupounds extensively and with good success. I believe that the repliset is a silicone material and it stays very flexible. A positive can be made with Repliset on a Repliset original replica if you don't let the polymer cure too long. Most of the other replicating materials that I have used cannot be used to make a positive from a like material. The Repliset material is very easy to work with in that it comes with a very nice mixing dispenser. The down side of the Repliset is a relatively high cost.
The dental casting compounds and similar products sold as machinist mold making compounds (ReproRubber Thin Pour Metrology Grade) also work well and are much cheaper. I have done comparisons using SEM and quantitative surface measurements of Repliset and ReproRubber and found good correlations with the original surfaces and the replicas for features in the size range that you would be seeing at 1000X.
I have also used acrylic compounds, but this material would be too stiff and brittle for replicating surfaces with undercuts. I expect that Epothin epoxy may have the same problem, but I have never used this material or other epoxies for replicas. Acrylics also have a problem with beam damage in the SEM. Some of these materials, such as the silicones, will outgas so watch your total replica size, especially if you will be using a high vacuum SEM.
Hope this helps.
} I am beginning a project that will involve the replication of fossil } and rock surfaces for examination under the SEM (gold coated, in most } cases well under 1000X). I was going to try and use a replication } compound made by Struers (Repliset) and a dental casting compound } Examix NDS (Hydrophilic Vinyl Polysiloxane). For making positive } replicas I was going to try Epothin epoxy and SPI positive replica } powder (polyethylene homopolymer), and the casting compounds } themselves. Does anyone have experience using any of these compounds } or know of better alternatives? The casts need to be flexible so that } they can be removed from undercuts. The epoxy I have read about is } Araldite, I could not find out what model number they used, or if it } is some kind of special epoxy? Thanks, Have a good holiday! } -David }
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
==============================Original Headers============================== 8, 22 -- From hanke-at-mee-inc.com Mon Dec 19 16:52:42 2005 8, 22 -- Received: from mail7.atl.registeredsite.com (mail7.atl.registeredsite.com [64.224.219.81]) 8, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBJMqfZq019698 8, 22 -- for {microscopy-at-microscopy.com} ; Mon, 19 Dec 2005 16:52:42 -0600 8, 22 -- Received: from netmail.mail.registeredsite.com (netmail2.mail.registeredsite.com [216.122.69.15]) 8, 22 -- by mail7.atl.registeredsite.com (8.12.11/8.12.11) with ESMTP id jBJMqe1K013875 8, 22 -- for {microscopy-at-microscopy.com} ; Mon, 19 Dec 2005 17:52:40 -0500 8, 22 -- Received: (qmail 76283 invoked by uid 89); 19 Dec 2005 23:02:56 -0000 8, 22 -- Received: from unknown (HELO ?192.168.1.109?) (216.43.123.204) 8, 22 -- by mail.saharagrp.com with SMTP; 19 Dec 2005 23:02:56 -0000 8, 22 -- Message-ID: {43A739B7.8060607-at-mee-inc.com} 8, 22 -- Date: Mon, 19 Dec 2005 16:52:39 -0600 8, 22 -- From: Larry Hanke {hanke-at-mee-inc.com} 8, 22 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 8, 22 -- X-Accept-Language: en-us, en 8, 22 -- MIME-Version: 1.0 8, 22 -- To: dwaugh-at-kent.edu, microscopy-at-microscopy.com 8, 22 -- Subject: Re: AskAMicroscopist: SEM sample replication 8, 22 -- References: {200512191415.jBJEFBwk020054-at-ns.microscopy.com} 8, 22 -- In-Reply-To: {200512191415.jBJEFBwk020054-at-ns.microscopy.com} 8, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rk.tiwari-at-ncl.res.in as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rk.tiwari-at-ncl.res.in Name: rajkiran
Organization: national chemical laboratory
Title-Subject: [Filtered] ultramicrotomy
Question: Hi all, I am Rajkiran R Tiwari from NCL, Pune. I am doing microtoming mostly on polymer samples at room temp. I have some doubts which I want to clarify. I am mentioning them below: 1. I usually keep diamond knife at 5 deg. whether it is ok?? 2. Even after fine polishing the samples with glass knife, the sections with diamond knife doesn't come uniformly.. Like in one round full trapezoid will get and in next cutting only part of trapezoid will cut. What may be reason for it? 3. I cut the sample 100nm and below with diamond knife. Should I polish the sample again with diamond knife at 500nm to make tarpezoid surface uniform?? 4. Which side of grid should be used to to collect sample, since in literature people use generally shiny side. Does mess also affect the imaging. Is 400 mess grid is ok to collect the sections?
Good morning, good afternoon, good evening (as applicable)
Dear Listers, ( see also general informations on SCUR-Meeting 2006 below )
dear R. R. Tiwari,
I am not able now to comment on your questions because it might need some time to formulate "reasons" (there are some) why you are facing the problems you describe. Unfortunately I am not aware of a publication in English commenting generally on } Ultramicrotomy, frequent problems and faults/imperfections {.
I only do have and could provide you with a 12 pages GERMAN publication on that, written by one of the "fathers" of modern ultramicrotomy, H. SITTE (January 1982: Ultramikrotomie - Haeufige Probleme und Fehler, GIT Verlg Darmstadt ). If you would like to have a .pdf of that publication, please let me know.
All the best wishes to you and yours, MERRY CHRISTMAS/Seasons Greetings and A HAPPY, HEALTHY and PROSPEROUS NEW YEAR 2006
best regards
Wolfgang MUSS Salzburg, Austria ( http://www.salzburgcb.com/en/salzburg/salzburg.htm )
I am still alive but again struggling and fighting now for the further existence of the EM-Lab here (February 2nd 2006 it still will have operated 25 years).
OR Dr. Wolfgang Muss Member of MSA, SUP, SCUR, FRMS..... EM-Lab Institute of Pathology, SALK (Salzburger Landeskliniken gemeinnuetzige GesmbH) Muellner Hauptstrasse 48 A-5020 SALZBURG AUSTRIA/EUROPE
and/or/alternatively Paracelsus Medical Private University (PMU) Institute of Pathology - Electron Microscopy Lab Muellner Hauptstrasse 48 A-5020 SALZBURG, Austria/Europe Phone work: +43+662+4482+4720 Mobile phone work:+43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss") E-Mail work: W.Muss-at-SALK.at
---------------------------------------------------------------------- ------- Information on behalf of Society for Cutaneous Ultrastructure Research (SCUR) PLEASE VISIT THE UPDATED WEBSITE of SCUR at } http://www.scur.org.pl { ------------------------------------------------------------------------- Forthcoming Meetings: 33rd Annual Meeting of the SCUR, 8th-10th June, 2006, WARSZAW, Poland Additional Informations: send an E-Mail kwoznia-at-amwaw.edu.pl
34th Annual Meeting of the SCUR, 11th-12th May, 2007, PRAGUE Czech Republic
35th Annual Meeting of the SCUR, 11th -12th May, 2008, NARA or KYOTO, Japan Joint Meeting with the JSUCB, the Japanese Society for Ultrastructural Cutaneous Biology
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Title-Subject: [Filtered] ultramicrotomy
Question: Hi all, I am Rajkiran R Tiwari from NCL, Pune. I am doing microtoming mostly on polymer samples at room temp. I have some doubts which I want to clarify. I am mentioning them below: 1. I usually keep diamond knife at 5 deg. whether it is ok?? 2. Even after fine polishing the samples with glass knife, the sections with diamond knife doesn't come uniformly.. Like in one round full trapezoid will get and in next cutting only part of trapezoid will cut. What may be reason for it? 3. I cut the sample 100nm and below with diamond knife. Should I polish the sample again with diamond knife at 500nm to make tarpezoid surface uniform?? 4. Which side of grid should be used to to collect sample, since in literature people use generally shiny side. Does mess also affect the imaging. Is 400 mess grid is ok to collect the sections?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (yjzhang-at-ciac.jl.cn) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, December 21, 2005 at 08:08:46 ---------------------------------------------------------------------------
Email: yjzhang-at-ciac.jl.cn Name: Yuanjian Zhang
Organization: Chinese Academy of Sciences
Education: Graduate College
Location: Changchun, Jilin, P.R. China
Question: Hi,
I want to quantify the elements (standardless) from EDAX spectra (created by GENESIS Software, EDAX, Inc in .spc format). But EDAX, Inc only supplies free Spectrum Viewer software, which can not do quantification. I am outside of my institute now, and then I wonder is there another way to quantify from these spectra by myself?
I regret to inform you that John C. Wheatley, lab manager for the John M. Cowley Center for High Resolution Electron Microscopy at Arizona State University, died on Sunday, December 18th, 2005. John worked in the field of electron microscopy for over 35 years and was a long-time member of this list.
A Celebration & Memorial Service for John Wheatley will be held at
First Baptist Church of Tempe 4525 S McClintock Dr Tempe, AZ 85282 (480) 839-0926 SE Corner of McClintock and US-60
On Thursday, December 22nd, at 7:00 PM
Flowers to the above or Mrs. Peggy Wheatley 1139 W Madero Circle Mesa, AZ 85210
e-Paul Paul R. Perkes Principal Technical Support Analyst Arizona State University Center for Solid State Science Phone: (480) 965-5218 Wireless: (602) 999-4781 E-mail: paul.perkes-at-asu.edu
==============================Original Headers============================== 2, 26 -- From Paul.Perkes-at-asu.edu Wed Dec 21 11:38:47 2005 2, 26 -- Received: from post5.inre.asu.edu (post5.inre.asu.edu [129.219.110.120]) 2, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBLHck7P010788 2, 26 -- for {microscopy-at-microscopy.org} ; Wed, 21 Dec 2005 11:38:46 -0600 2, 26 -- Received: from conversion.post5.inre.asu.edu by asu.edu (PMDF V6.1-1X6 #30769) 2, 26 -- id {0IRU00601ZJQK7-at-asu.edu} for microscopy-at-microscopy.org; Wed, 2, 26 -- 21 Dec 2005 10:35:50 -0700 (MST) 2, 26 -- Received: from EX02.asurite.ad.asu.edu 2, 26 -- (excl0-a1.asurite.ad.asu.edu [129.219.12.200]) 2, 26 -- by asu.edu (PMDF V6.1-1X6 #30769) with ESMTP id {0IRU005NXZJQCE-at-asu.edu} for 2, 26 -- microscopy-at-microscopy.org; Wed, 21 Dec 2005 10:35:50 -0700 (MST) 2, 26 -- Date: Wed, 21 Dec 2005 10:35:53 -0700 2, 26 -- From: Paul Perkes {Paul.Perkes-at-asu.edu} 2, 26 -- Subject: Death of John C. Wheatley 2, 26 -- To: microscopy-at-microscopy.org 2, 26 -- Message-id: {7CB75848EFAAC84D81B000C6A3A35178A6BF17-at-EX02.asurite.ad.asu.edu} 2, 26 -- MIME-version: 1.0 2, 26 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 2, 26 -- Content-type: text/plain; charset=us-ascii 2, 26 -- Thread-Topic: Death of John C. Wheatley 2, 26 -- Thread-Index: AcYGVP3eGdYF9qR3SQ6/gsIZ2Z0vnw== 2, 26 -- Content-class: urn:content-classes:message 2, 26 -- X-MS-Has-Attach: 2, 26 -- X-MS-TNEF-Correlator: 2, 26 -- Content-Transfer-Encoding: 8bit 2, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jBLHck7P010788 ==============================End of - Headers==============================
1. Diamond knife angle Most diamond knifes have a setting of 4 degress, but then again Ihave seen a variation in this angle. The setting is usually mentioned on the original dimond knife box. 2. Glass knife and incomplete trapezoids Are polishing your block face with the glass knife at the same angle as that of the diamond knife? If not, that might be the reason for you incomplete sections 3. Grid surface It is different for different grids.If you are using a formvar coated grid then you collect sections on the shiny side, while using a copper grid (uncoated) you collect on the dull side.
Hope that was helpful. regards, Vinod Nair Graduate Student Dept. of Biology New mexico State University
On 12/20/05, rk.tiwari-at-ncl.res.in {rk.tiwari-at-ncl.res.in} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both rk.tiwari-at-ncl.res.in as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: rk.tiwari-at-ncl.res.in } Name: rajkiran } } Organization: national chemical laboratory } } Title-Subject: [Filtered] ultramicrotomy } } Question: Hi all, } I am Rajkiran R Tiwari from NCL, Pune. } I am doing microtoming mostly on polymer samples at room temp. I have some doubts which I want to clarify. } I am mentioning them below: } 1. I usually keep diamond knife at 5 deg. whether it is ok?? } 2. Even after fine polishing the samples with glass knife, the sections with diamond knife doesn't come uniformly.. Like in one round full trapezoid will get and in next cutting only part of trapezoid will cut. What may be reason for it? } 3. I cut the sample 100nm and below with diamond knife. Should I polish the sample again with diamond knife at 500nm to make tarpezoid surface uniform?? } 4. Which side of grid should be used to to collect sample, since in literature people use generally shiny side. Does mess also affect the imaging. Is 400 mess grid is ok to collect the sections? } } Kindly help me by clarifying the doubts. } } with regards } Rajkiran } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 12 -- From zaluzec-at-microscopy.com Tue Dec 20 18:06:04 2005 } 8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBL06205000790 } 8, 12 -- for {microscopy-at-microscopy.com} ; Tue, 20 Dec 2005 18:06:03 -0600 } 8, 12 -- Mime-Version: 1.0 } 8, 12 -- X-Sender: (Unverified) } 8, 12 -- Message-Id: {p06110401bfce4cd8790d-at-[206.69.208.22]} } 8, 12 -- Date: Tue, 20 Dec 2005 18:06:01 -0600 } 8, 12 -- To: microscopy-at-microscopy.com } 8, 12 -- From: rk.tiwari-at-ncl.res.in (by way of MicroscopyListserver) } 8, 12 -- Subject: [Filtered] MicroscopyListserverviaWWW: ultramicrotomy questions } 8, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 25 -- From nairvinods-at-gmail.com Wed Dec 21 11:48:37 2005 5, 25 -- Received: from zproxy.gmail.com (zproxy.gmail.com [64.233.162.202]) 5, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBLHmX1E012735 5, 25 -- for {microscopy-at-microscopy.com} ; Wed, 21 Dec 2005 11:48:35 -0600 5, 25 -- Received: by zproxy.gmail.com with SMTP id s18so254509nze 5, 25 -- for {microscopy-at-microscopy.com} ; Wed, 21 Dec 2005 09:48:32 -0800 (PST) 5, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 25 -- s=beta; d=gmail.com; 5, 25 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 5, 25 -- b=IHiViVk85wBtO8l+LXGTwq2GdAB7ln4DgQAT1KTGnns1qpP1RiIZxRdrtXtHooaERZZe7amKpWNBzqWk+rDqnVCFU8wpu7gd3wBmTj1rQAzfOWeK+xQdf/yB3WshBx5ao+u0VoVyUEOoN4MgA+U6pjipMvGavTMoq+3FPXgWjCY= 5, 25 -- Received: by 10.64.203.5 with SMTP id a5mr693136qbg; 5, 25 -- Wed, 21 Dec 2005 09:48:32 -0800 (PST) 5, 25 -- Received: by 10.64.201.14 with HTTP; Wed, 21 Dec 2005 09:48:32 -0800 (PST) 5, 25 -- Message-ID: {ea42a3900512210948n3a331926m30178f7b9bbc04c9-at-mail.gmail.com} 5, 25 -- Date: Wed, 21 Dec 2005 10:48:32 -0700 5, 25 -- From: Vinod Nair {nairvinods-at-gmail.com} 5, 25 -- To: microscopy-at-microscopy.com 5, 25 -- Subject: Re: [Microscopy] [Filtered] MicroscopyListserverviaWWW: ultramicrotomy questions 5, 25 -- In-Reply-To: {200512210051.jBL0pAwt009376-at-ns.microscopy.com} 5, 25 -- MIME-Version: 1.0 5, 25 -- Content-Type: text/plain; charset=UTF-8 5, 25 -- Content-Disposition: inline 5, 25 -- References: {200512210051.jBL0pAwt009376-at-ns.microscopy.com} 5, 25 -- Content-Transfer-Encoding: 8bit 5, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id jBLHmX1E012735 ==============================End of - Headers==============================
Anyone ever have problems using permount and FITC? I was asked if there was any issues about transmitability or other problems that could arise. I had no evidence to support my premise but it seemed that to me that the index of refraction shouldn't present a problem so it should work?
Happy Holidays to all you Listers!
Histology/Electron Microscopy Technician Department of Anatomy and Cell Biology Queen's University Kingston, Ontario K7L 3N6 Phone: 613- 533 6000 x 78265
==============================Original Headers============================== 4, 15 -- From nicholls-at-post.queensu.ca Thu Dec 22 09:44:13 2005 4, 15 -- Received: from post.queensu.ca (post.QueensU.CA [130.15.126.6]) 4, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBMFiC5K023260 4, 15 -- for {microscopy-at-microscopy.com} ; Thu, 22 Dec 2005 09:44:12 -0600 4, 15 -- Received: from EMSuite.post.queensu.ca (U55.N149.QueensU.CA [130.15.149.55]) 4, 15 -- by post.queensu.ca (8.13.1/8.13.1) with ESMTP id jBMFiBNt000125 4, 15 -- for {microscopy-at-microscopy.com} ; Thu, 22 Dec 2005 10:44:12 -0500 (EST) 4, 15 -- Message-Id: {6.2.3.4.0.20051222103523.01d51f88-at-post.queensu.ca} 4, 15 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 4, 15 -- Date: Thu, 22 Dec 2005 10:44:25 -0500 4, 15 -- To: microscopy-at-microscopy.com 4, 15 -- From: Rod Nicholls {nicholls-at-post.queensu.ca} 4, 15 -- Subject: Permount and FITC 4, 15 -- Mime-Version: 1.0 4, 15 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Just wonder if anyone has a tip on a good (commercially available) antibody against His tag for EM - Tokuyasu technique. Should tolerate at least light (0.1-0.2%) glutaraldehyde fixation. A rabbit polyclonal would be preferable, but a working monoclonal would do as well.
Another unrelated question - I would need to label macrophages in sections (again, Tokuyasu). Any idea for a good macrophage marker?
Thanks and happy Holidays,
Michal
==============================Original Headers============================== 5, 19 -- From M_Jarnik-at-fccc.edu Thu Dec 22 10:02:57 2005 5, 19 -- Received: from azah.fccc.edu (azah.fccc.edu [131.249.4.237]) 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBMG2t5J026707 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 22 Dec 2005 10:02:56 -0600 5, 19 -- Received: from fccc.edu (emf4.fccc.edu [131.249.9.170]) 5, 19 -- by azah.fccc.edu (8.12.11/8.12.11) with ESMTP id jBMG2taq015813 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 22 Dec 2005 11:02:55 -0500 (EST) 5, 19 -- Message-ID: {43AACE3E.7060404-at-fccc.edu} 5, 19 -- Date: Thu, 22 Dec 2005 11:03:10 -0500 5, 19 -- From: Michal Jarnik {M_Jarnik-at-fccc.edu} 5, 19 -- Reply-To: M_Jarnik-at-fccc.edu 5, 19 -- Organization: Fox Chase Cancer Center 5, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 5, 19 -- X-Accept-Language: en,cs 5, 19 -- MIME-Version: 1.0 5, 19 -- To: microscopy-at-microscopy.com 5, 19 -- Subject: His Tag antibody for EM 5, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 5, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello all, I have a Reichert-Jung Frigocut 2800 cryostat and I need to replace the light bulb.
Radium Relux 11W/21 Germany 2x4
Haven't had any luck doing a google search so any advice would be greatly appreciated. happy holidays, Beth
********************************************************************** Beth Richardson EM Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
One doesn't generally used organic solvent based mounting media with fluorochrome labeled specimens. The xylene in Permount would destroy the fluorescence. You need one of the many commerical preparations (e.g., Prolong Gold from Molecular Probes) or Mowiol (google for the recipe on line). Good luck.
At 09:54 AM 12/22/05, you wrote:
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Thanks for all the suggestions! Calling companies worked the best rather than trying websites. The folks at Bulbman were very helpful. I appreciate the advice! best, Beth
********************************************************************** Beth Richardson EM Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
==============================Original Headers============================== 8, 18 -- From beth-at-plantbio.uga.edu Thu Dec 22 15:40:47 2005 8, 18 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 8, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBMLekiO031833 8, 18 -- for {microscopy-at-microscopy.com} ; Thu, 22 Dec 2005 15:40:47 -0600 8, 18 -- Received: from plantbio.uga.edu ([128.192.26.46]) 8, 18 -- by dogwood.plantbio.uga.edu 8, 18 -- (using TLSv1/SSLv3 with cipher DES-CBC3-SHA (168 bits)) 8, 18 -- for microscopy-at-microscopy.com; 8, 18 -- Thu, 22 Dec 2005 16:40:41 -0500 8, 18 -- Date: Thu, 22 Dec 2005 16:40:43 -0500 8, 18 -- Mime-Version: 1.0 (Apple Message framework v553) 8, 18 -- Content-Type: text/plain; delsp=yes; charset=US-ASCII; format=flowed 8, 18 -- Subject: thanks - cryostat bulb 8, 18 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 8, 18 -- To: microscopy microscopy {microscopy-at-microscopy.com} 8, 18 -- Content-Transfer-Encoding: 7bit 8, 18 -- Message-Id: {9AC1BCBE-7333-11DA-88D0-000393137C00-at-plantbio.uga.edu} 8, 18 -- X-Mailer: Apple Mail (2.553) ==============================End of - Headers==============================
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Hi thanks to all for their valuable suggestions.
rajkiran
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==============================Original Headers============================== 5, 26 -- From rk.tiwari-at-ncl.res.in Fri Dec 23 00:39:52 2005 5, 26 -- Received: from chandra.ncl.res.in ([202.54.11.141]) 5, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBN6doxZ019378 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 23 Dec 2005 00:39:51 -0600 5, 26 -- Received: from raman (raman.ncl.res.in [172.16.2.25]) 5, 26 -- by chandra.ncl.res.in (8.12.8/8.12.8) with ESMTP id jBN6s51h021650 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 23 Dec 2005 12:24:06 +0530 5, 26 -- Received: (qmail 20269 invoked by uid 0); 23 Dec 2005 06:21:48 -0000 5, 26 -- Received: from 172.16.53.190 by sandesh.ncl.res.in (envelope-from {rk.tiwari-at-ncl.res.in} , uid 0) with qmail-scanner-1.25 5, 26 -- (clamscan: 0.60. 5, 26 -- Clear:RC:0(172.16.53.190):. 5, 26 -- Processed in 0.866507 secs); 23 Dec 2005 06:21:46 -0000 5, 26 -- Received: from unknown (HELO [127.0.0.1]) (rk.tiwari-at-ncl.res.in-at-[172.16.53.190]) 5, 26 -- (envelope-sender {rk.tiwari-at-ncl.res.in} ) 5, 26 -- by 0 (qmail-ldap-1.03) with SMTP 5, 26 -- for {microscopy-at-microscopy.com} ; 23 Dec 2005 06:21:45 -0000 5, 26 -- Message-ID: {43AB9BBF.5010900-at-ncl.res.in} 5, 26 -- Date: Fri, 23 Dec 2005 12:09:59 +0530 5, 26 -- From: Rajkiran Tiwari {rk.tiwari-at-ncl.res.in} 5, 26 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 5, 26 -- X-Accept-Language: en-us, en 5, 26 -- MIME-Version: 1.0 5, 26 -- To: microscopy-at-microscopy.com 5, 26 -- Subject: thanks 5, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
David Waugh wrote: ===================================================== I am beginning a project that will involve the replication of fossil and rock surfaces for examination under the SEM (gold coated, in most cases well under 1000X). I was going to try and use a replication compound made by Struers (Repliset) and a dental casting compound Examix NDS (Hydrophilic Vinyl Polysiloxane). For making positive replicas I was going to try Epothin epoxy and SPI positive replica powder (polyethylene homopolymer), and the casting compounds themselves. Does anyone have experience using any of these compounds or know of better alternatives? The casts need to be flexible so that they can be removed from undercuts. The epoxy I have read about is Araldite, I could not find out what model number they used, or if it is some kind of special epoxy? ===================================================== The selection of a replication system depends on your application.
For example, a) ultrafast cure can be important, such as for the replication of human skin (and resolution is less important) b) high resolution characteristics of the replication resin can be the most important c) the surface chemical characteristics and adhesion characteristics can be important so that when the replica is removed, it does not remove part of the sample with it. d) dimensional integrity of the replica can be important as is needed in dentistry but not necessarily so much in microscopy d) robust enough so that when a positive replica is made, fine features on the silicone don't get pulled off by the positive when separated.
For the replication of geological surfaces, we have found the SPI "Wet Replica" kit to work nicely, but there are trade offs. You will get less resolution in the negative (vs. epoxy), but when it lifts off, it is the least likely of the possibilities to remove surface debris and fine features just barely hanging onto the surface. In order to get the replicating silicone "into" the sample, we sometimes use a "duster" to sort of help blast the liquid into the crevices, etc. Now obviously there are limits to what one can do, even with this particular silicone, but it seems to work quite well. But since the silicone at that point and in its cured state, might be tissue-paper thin, very fragile and hard to handle, there is nothing that prevents one from adding a "second stage', that is, another layer of the resin to give the high resolution "film" more dimensional rigidity and overall making it much less fragile. Since the resin is white, the picking off of pieces from the sample which usually show up "black" can be used as an assessment as to how much surface material did get removed by the resin. You can also look at the generally dark colored or black geological sample and see how much "white" is showing up as a measure of how much of the replica could not be pulled off of the surface.
The downside to the SPI wet replica kit is that after about 700x in the SEM, one starts to see artifact structure from the replicating resin itself.
Other information about the SPI Wet Replica Kit can be found at URL http://www.2spi.com/catalog/spec_prep/wet_rep_kits.shtml
We like the low MW polyolefin "positive" replicating powder which leads to a good positive replica of the surface. It also separates very easily from the silicone negative, even when the positive material has to go into small crevices and convolutions on the negative replica surface.
Disclaimer: SPI Supplies manufactures the SPI Wet Replica Kit.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.2spi.com ######################## ============================================
==============================Original Headers============================== 15, 30 -- From cgarber-at-2spi.com Sun Dec 25 16:26:51 2005 15, 30 -- Received: from s-utl02-dcpop.stsn.net (s-utl02-dcpop.stsn.net [72.255.0.202]) 15, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id jBPMQp7j031277 15, 30 -- for {microscopy-at-msa.microscopy.com} ; Sun, 25 Dec 2005 16:26:51 -0600 15, 30 -- Received: from s-utl02-dcpop.stsn.net ([127.0.0.1]) 15, 30 -- by s-utl02-dcpop.stsn.net (SMSSMTP 4.1.2.20) with SMTP id M2005122517265018020 15, 30 -- for {microscopy-at-msa.microscopy.com} ; Sun, 25 Dec 2005 17:26:50 -0500 15, 30 -- X-Spam-Status: No, hits=0.0 required=9.9 15, 30 -- tests=ALL_TRUSTED: -2.4,AWL: 0.222,SARE_RECV_ADDR: 0.027 15, 30 -- X-Spam-Level: 15, 30 -- Received: from ibm1x23g2abfyg ([10.6.28.167]) 15, 30 -- by s-utl02-dcpop.stsn.net 15, 30 -- for microscopy-at-msa.microscopy.com; 15, 30 -- Sun, 25 Dec 2005 17:26:49 -0500 15, 30 -- Message-ID: {027a01c609a2$4adbab60$a71c060a-at-ibm1x23g2abfyg} 15, 30 -- Reply-To: "Garber, Charles A." {cgarber-at-2spi.com} 15, 30 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 15, 30 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 15, 30 -- Subject: Replicating systems for SEM 15, 30 -- Date: Sun, 25 Dec 2005 17:26:37 -0500 15, 30 -- MIME-Version: 1.0 15, 30 -- Content-Type: text/plain; 15, 30 -- format=flowed; 15, 30 -- charset="Windows-1252"; 15, 30 -- reply-type=original 15, 30 -- Content-Transfer-Encoding: 7bit 15, 30 -- X-Priority: 3 15, 30 -- X-MSMail-Priority: Normal 15, 30 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 15, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
Thank you to all of you who responded to my inquiry. The general response was that you can mount them by mixing either an ethanol/methanol mixture or distilled water with the dry tubes and mount them either on lacey film or formvar, with the majority suggesting lacey film.
I tried both and had the best success with the formvar. Thank you all who gave your suggestions, they were invaluable.
Mike Ganger Weill Cornell Medical College
==============================Original Headers============================== 6, 20 -- From mganger-at-optonline.net Sun Dec 25 19:00:48 2005 6, 20 -- Received: from mta2.srv.hcvlny.cv.net (mta2.srv.hcvlny.cv.net [167.206.4.197]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBQ10lX6008832 6, 20 -- for {Microscopy-at-microscopy.com} ; Sun, 25 Dec 2005 19:00:47 -0600 6, 20 -- Received: from [127.0.0.1] (ool-43511f6b.dyn.optonline.net [67.81.31.107]) 6, 20 -- by mta2.srv.hcvlny.cv.net 6, 20 -- (Sun Java System Messaging Server 6.2-4.03 (built Sep 22 2005)) 6, 20 -- with ESMTP id {0IS200D5FYT9YFAJ-at-mta2.srv.hcvlny.cv.net} for 6, 20 -- Microscopy-at-microscopy.com; Sun, 25 Dec 2005 20:00:47 -0500 (EST) 6, 20 -- Date: Sun, 25 Dec 2005 20:00:43 -0500 6, 20 -- From: Michael Ganger {mganger-at-optonline.net} 6, 20 -- Subject: Carbon NanoTubes Thanks 6, 20 -- To: Microscopy List Server {Microscopy-at-microscopy.com} 6, 20 -- Message-id: {43AF40BB.8010401-at-optonline.net} 6, 20 -- MIME-version: 1.0 6, 20 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-transfer-encoding: 7BIT 6, 20 -- X-Accept-Language: en-us, en 6, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 6, 20 -- Gecko/20040804 Netscape/7.2 (ax) ==============================End of - Headers==============================
Hi all, We are trying to do Ecoli fixate. I am looking for a procedure of how to make polylysine coverslips and slides. I would also like to know if these are commercially available.
Thanks
Eli
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lubo-at-berkeley.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: lubo-at-berkeley.edu Name: Lubo
Organization: UCB
Title-Subject: [Filtered] Trasmitted light in scanning mode: Zeiss LSM510 (meta)
Question: Hello, I'd like to get a brightfield image - polarized light - subsequently after a confocal image is taken from the same specimen. Trivial enough.
Here is the problem: the Zeiss LSM510 (meta) we have just shuts the transmitted light off once the lever is pulled all the way out (in the LSM - scanning mode). According to the manual having the transmitted light on while acquiring an image is a feasible thing to do. The light path is set correctly I think: Microscope Control panel } transmitted light On, Field Stop iris needs & Filter at 100%, tried with variety of transmitted light settings from 100-0%.
I wonder if shutting the transmitted light off once the light pass lever is pulled out for the scanning mode is a software bug or there is some obscure setting I'm missing?
This is a message posted at the request of a colleague.
A ZEISS EM-900 TEM is available for a good home that probably just needs to cover the cost for dismantling and shipping.
For more information, please contact Dr. Shu-Chun Su at
SSu-at-Herc.com (302) 995-3498 (phone)
**************************************** Chaoying Ni The W.M. Keck Electron Microscope Facility Facility location: 022 Spencer Laboratory Mailing address: 201 duPont Hall Dept of Matls Sci & Eng University of Delaware Newark, DE 19716 (302) 831-2318(L); -4545(Fax) http://eml.masc.udel.edu *****************************************
==============================Original Headers============================== 6, 18 -- From cni-at-UDel.Edu Wed Dec 28 09:05:18 2005 6, 18 -- Received: from copland.udel.edu (copland.udel.edu [128.175.13.92]) 6, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBSF5I9r011833 6, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Dec 2005 09:05:18 -0600 6, 18 -- Received: from copland.udel.edu (localhost [127.0.0.1]) 6, 18 -- by copland.udel.edu (8.12.11/8.12.11) with ESMTP id jBSF5Hmr011267 6, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Dec 2005 10:05:17 -0500 (EST) 6, 18 -- Received: from localhost (cni-at-localhost) 6, 18 -- by copland.udel.edu (8.12.11/8.12.11/Submit) with ESMTP id jBSF5HWs011264 6, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Dec 2005 10:05:17 -0500 (EST) 6, 18 -- Date: Wed, 28 Dec 2005 10:05:17 -0500 (EST) 6, 18 -- From: Chaoying Ni {cni-at-UDel.Edu} 6, 18 -- To: Microscopy-at-microscopy.com 6, 18 -- Subject: ZEISS EM-900 TEM for good home 6, 18 -- Message-ID: {Pine.SOL.4.60L.0512280950390.9952-at-copland.udel.edu} 6, 18 -- MIME-Version: 1.0 6, 18 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 6, 18 -- X-Scanned-By: MIMEDefang 2.52 on 128.175.13.92 ==============================End of - Headers==============================
Here is the January 2006 Microscopy Today table of contents. I will close the subscription list for this issue on Tuesday, January 3, 2006.
Microscopists in North America and MSA members anywhere may have free subscriptions. Anyone else may subscribe for US$50 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com Thank you.
Ron Anderson, Editor =================== Why Flies Walk with Wet Feet Stephen W. Carmichael, Mayo Clinic
Advanced Confocal Microscopy An Essential Technique for Microfluidics Development Terence Lundy, Hyphenated-Systems, Burlingame, CA
The Staining of Polymers II R. W. Smith and V. Bryg,* Lake Havasu City, AZ and *Richfield, OH
Low Voltage FESEM of Geological Materials C. Ma and G. Rossman, California Institute of Technology, Pasadena, CA
Temperature Monitoring of an EM Environment D. Fellmann, R. Bañez, B. Carragher and C. S. Potter, The Scripps Research Institute, La Jolla, CA
Practical Issues for Quantitative X-ray Microanalysis in SEM at Low kV Peter Statham, Oxford Instruments Analytical Limited, High Wycombe, Bucks U.K.
Mounting Media and Antifade Reagents Compiled by Tony J. Collins, Wright Cell Imaging Facility, Toronto Western Research Institute, Canada
Ethics and Digital Imaging J. M. Mackenzie, M. G. Burke, T. Carvalho and A. Eades, MSA Sub Committee on the Ethics of Digital Imaging
Investigating the Microstructure of a Newly Developed Aluminum Alloy Through X-ray Microanalysis P. Camus and D. Rohde, Thermo Electron Corporation, Madison, WI
Fostering LIMS Development Through Open Standards: Part II – Ontologies and Business Process Avrum Goodblatt, PathBioResource, U. PENN School of Medicine
Freezing Biological Samples Charles W. Scouten & Miles Cunningham, myNeuroLab.com, St. Louis, MO
Industry News
NetNotes
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both susan.vanhorn-at-stonybrook.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: susan.vanhorn-at-stonybrook.edu Name: Sue Van Horn
Organization: SUNY-at-StonyBrook
Title-Subject: [Filtered] Potassium Permanganate
Question: I am looking for a protocol using potassium permanganate to enhance membrane contrast...any suggestions??? thanks sue
dear Sue, unfortunately you did not specify wether you look for a fixation OR a staining protocol nor which tissue type (human, animal, plant tissue) you are going to investigate. May I add some Infos for you, hoping that you will communicate the answer results of your questions to me too. Thank you in advance for this.
Therefore I cite: PLATTNER H., ZINGSHEIM H.P: Elektronenmikroskopische Methodik in der Zell- und Molekularbiologie (GERMAN, G. FISCHER, Stuttgart, 1987), ISBN 3-437-30494-1, pp. 41 ff, 282, 284
Version 1). by KMnO4-Fixation: unfortunately I do not have at hand a protocol which was described for human tissue fixation.....but - meanwhile I was writing this message, my PC-"search programme" has found some references and articles dealing with KMnO4 either as fixation or as contrast/staining agent, at least one in NetNotes in Micr. Today, 13, No 3, p. 66 - 67 (May 2005), a word.doc I could send to you and everybody requesting such documents.
} } translated { { Permanganate-ions (Mn(VII)O -4) react similarly like OsO4. KMnO4 as a fixative was introduced by LUFT 1956 for animal tissue and MOLLENHAUER 1959 for EM of plant cells and tissues. The origial fixation technique still is in use with quite good results for plant tissue, since the fixative penetrates the cell wall very well leaving the cells undestroyed by the cell wall structure. Animalic cells and tissues most often will be destroyed or at least be damaged by KMnO4 especially if one does not take special care in the fixation procedure [ no specification given which parameters therefore are important ]. Ribosomes under any circumstances will be destroyed completely. On the other hand, KMnO4 produces well stained "unit-membranes" without the necessity of a further staining the ultrathin sections. The "term" } } unit-membrane { { originally was deduced from permanganate-fixed material (ROBERTSON 1958)
KMnO4-FIXATION (MOLLENHAUER 1959) for plant tissue Pretreatment: reduce size of specimen to 0.3 mm x 0.3mm x 0.3 mm Method: fixative consists of 2-5 % KMno$-solution, either unbuffered or buffered with Veronal-Acetate to a pH of 6.0. Fixation at 0?C or room temperature, for some minutes up to 2 h. Tips & Results: heavy membrane contrast. Secondary staining usually not necessary. Ribosomes totally are destroyed/unvisible. For washing one should use either buffer solution or 25% dehydration medium, e.g. Aceton. Fixation for animal tissue possible, but sometime this results in destroyed overall ultrastructural preservation of cellular structures.
Version 2) KMnO4 as a staining agent for ultrathin sections } } BRAY & WAGENAAR (1978) have reinstated the section staining by permanganate. The combination with alkaline Pb-citrate section staining will result in well stained biomembranes. Method (BRAY and WAGENAAR 1978): 1% unbuffered KMnO4 (hydrous) solution to be applicated 5-20 min (-at-room temperature = RT). Important to know: grids with sections should be immersed in the solution by pushing them in a vertical orientation through the KMnO4-solution's surface [my personal note: cave: surface tension, perhaps formvar mounting will be destroyed]. For LowicrylK4M sections, Plattner&Zingsheim recommend [article KELLENBERGER et al. 1980, PLT-progressive lowering temperature-technique for L-4KM] also 1% (w/vol) hydrous KMnO4 for 5-10 min -at- RT After incubation pull out grids also in a vertical orientation, wash thoroughly with/in A. bidest and counterstain with alkaline Pb-citrate (e.g. Reynolds, Venable&Coggeshall).
The complete bibliographic references as cited here you can get on request.
Hope that you will be lucky with your results
ALL BEST WISHES for a HAPPY, HEALTHY, PROSPEROUS and SUCCESSFUL NEW YEAR 2006
best regards,
Wolfgang MUSS SALZBURG, AUSTRIA
OR Dr. Wolfgang Muss Member of MSA, FRMS.... EM-Lab Institute of Pathology, SALK (Salzburger Landeskliniken gemeinnuetzige GesmbH) Muellner Hauptstrasse 48 A-5020 SALZBURG AUSTRIA/EUROPE ----------- and/or/alternatively ------------- Paracelsus Medical Private University (PMU) Institute of Pathology Electron Microscopy Lab Muellner Hauptstrasse 48 A-5020 SALZBURG, Austria/Europe Phone work: +43+662+4482+4720 Mobile phone work:+43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss") E-Mail work: W.Muss-at-SALK.at Mobile-phone private: ++43+676+5 369-456 E-Mail private: wij.Muss-at-aon.at ------------------------------------------------------------------------ ------------------------------- Ankuendigung namens der (Information on behalf of) Society for Cutaneous Ultrastructure Research (SCUR) PLEASE VISIT THE UPDATED WEBSITE of SCUR at } http://www.scur.org { ------------------------------------------------------------------------- Forthcoming Meetings: 33rd Annual Meeting of the SCUR, 8th-10th June, 2006, WARSZAW, Poland WEBSITE, containing all FORMS: http://www.scur.org.pl Additional informations: send an E-Mail kwoznia-at-amwaw.edu.pl
34th Annual Meeting of the SCUR, 11th-12th May, 2007, PRAGUE Czech Republic
35th Annual Meeting of the SCUR, 11th -12th May, 2008, NARA or KYOTO, Japan Joint Meeting with the JSUCB, the Japanese Society for Ultrastructural Cutaneous Biology
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Question: I am looking for a protocol using potassium permanganate to enhance membrane contrast...any suggestions??? thanks sue
I grow tantalum oxide electrochemically on tantalum substrates, the issue is that using SEM I can say that the surface of tantalum oxide is full of defects "pin holes". I just use mechanical polishing of tantalum before growing oxide, so I think that these defects result from impurities of polishing sand papers. Is there any other way I can polish tantalum to the finest grade avoiding these impurities?
I appreciate your responses.
Thanks -- ********************************************************** Hany Ramadan Graduate student Chemistry department McMaster university, Hamilton, Ontario, Canada 905-525-9140 x: 26322 elsayeh-at-mcmaster.ca **********************************************************
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Pure Tantalum is soft and generally difficult to polish metallographically. Embedment of abrasive particles is one of the problems.
First, can you verify the problem of particle embedment by examining the surface after polishing and before growing the oxide?... either with a metallograph or using SEM/EDS?
Though I've personally never polished Tantalum, my source, "Metallography Principles and Practise" by George Vander Voort, recommends using a chemical attack-polish after diamond polishing to prepare soft Tantalum surfaces.
A number of recipes are offered:
1.
15 g fine alumina 35 ml water 5 ml 20% CrO3 in water napped cloth, 1750 rpm medium to heavy pressure recharge periodically
2.
Solution 1 2-5% aq. CrO3 alumina abrasive
Solution 2 50 ml lactic acid 30 ml HNO3 2 ml HF
napped cloth, 1750 rpm with solution 1 follow with chemical polishing with solution 2
3.
100 ml acetic acid 60 ml HNO3 3 ml HF alpha alumina polish through 6 micron diamond add solution to napped cloth, add dry abrasive use 30 psi pressure for 8 min then 10 psi for 1 min
Stu Smalinskas, P.E. Metallurgist SKF Plymouth, Michigan (734) 414-6862
--- ramadanhany-at-gmail.com wrote: } } Good morning, } } I grow tantalum oxide electrochemically on tantalum } substrates, the } issue is that using SEM I can say that the surface } of tantalum oxide } is full of defects "pin holes". I just use } mechanical polishing of } tantalum before growing oxide, so I think that these } defects result } from impurities of polishing sand papers. Is there } any other way I can } polish tantalum to the finest grade avoiding these } impurities? } } I appreciate your responses. } } Thanks } -- } ********************************************************** } Hany Ramadan } Graduate student } Chemistry department } McMaster university, Hamilton, Ontario, Canada } 905-525-9140 x: 26322 } elsayeh-at-mcmaster.ca } ********************************************************** }
__________________________________ Yahoo! for Good - Make a difference this year. http://brand.yahoo.com/cybergivingweek2005/
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1. 90% sulphuric, 10% hydrofluoric acid (40%), electropolish with C or Pt cathode at 12 to 20 V.
2. 5% sulphuric, 1.25% hydrofluoric acid (40%), 93.75% methanol, electropolish at 50-70 V.
3. 50% nitric, 50% hydrofluoric (48%), immersion chemical polish.
-- Larry Stoter
PLEASE NOTE 1. Any mail other than plain text will be automatically deleted. 2. Any mail, legitimate or not, apparently or actually from hotmail, netscape, yahoo or excite will automatically be deleted. 3. Mail with no subject or without a clear subject will be ignored :-)
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