Over the Christmas break we routinely shut our microscopes down to run only on ion pumps as our cooling water can be a little "temperamental".
This year we had a power glitch which turned the microscopes completely off. To top it off our cooling water is out of action for maintenance until next week!! I was hoping to get all the ion pumps running on the microscopes to keep the vacuums at leat a bit clean and I have managed to get this done on all but our JEOL 2010. Does anyone know if it is possible to get just the ion pumps running without needing cooling water on a 2010?
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==============================Original Headers============================== 17, 28 -- From Colin.Veitch-at-csiro.au Mon Jan 2 18:38:05 2006 17, 28 -- Received: from vic-ironport-ext-out1.csiro.au (vic-ironport-ext-out1.csiro.au [150.229.64.37]) 17, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k030c3V4024434 17, 28 -- for {microscopy-at-msa.microscopy.com} ; Mon, 2 Jan 2006 18:38:04 -0600 17, 28 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=xU3RCN8AXAmWEvu85jV1wrWTuUHnFiSlBXAaGGnTue1S/WJESDcqcwHjBKBfIWsOg4hoNydsUlK2UaXg0lctHoNuIRakZsSu0pTgT/UHQM1uofzGf4egwYNOnYE3LgVO; 17, 28 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 17, 28 -- by vic-ironport-ext-out1.csiro.au with ESMTP; 03 Jan 2006 11:38:02 +1100 17, 28 -- X-IronPort-AV: i="3.99,323,1131282000"; 17, 28 -- d="scan'208"; a="63973513:sNHT23878160" 17, 28 -- Received: from exvic5-gex.nexus.csiro.au ([138.194.194.6]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 17, 28 -- Tue, 3 Jan 2006 11:38:02 +1100 17, 28 -- content-class: urn:content-classes:message 17, 28 -- MIME-Version: 1.0 17, 28 -- Content-Type: text/plain; 17, 28 -- charset="us-ascii" 17, 28 -- Subject: Restarting a JEOL 2010 on ion pumps 17, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 17, 28 -- Date: Tue, 3 Jan 2006 11:38:02 +1100 17, 28 -- Message-ID: {32CDDDAA7161394599F0025494915749051DD3-at-exvic5-gex.nexus.csiro.au} 17, 28 -- X-MS-Has-Attach: 17, 28 -- X-MS-TNEF-Correlator: 17, 28 -- Thread-Topic: Restarting a JEOL 2010 on ion pumps 17, 28 -- Thread-Index: AcYP/fQ3/LdcT+y2Rj221MWzoKvg+g== 17, 28 -- From: {Colin.Veitch-at-csiro.au} 17, 28 -- To: {microscopy-at-msa.microscopy.com} 17, 28 -- X-OriginalArrivalTime: 03 Jan 2006 00:38:02.0959 (UTC) FILETIME=[F47CA5F0:01C60FFD] 17, 28 -- Content-Transfer-Encoding: 8bit 17, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k030c3V4024434 ==============================End of - Headers==============================
FOCUS ON MICROSCOPY 2006, Perth, Australia, April 9-12, 2006 19th International Conference on 3D Image Processing in Microscopy 18th International Conference on Confocal Microscopy
Dear Colleagues
January 9, 2006, the deadline for abstract submission for the Perth conference is nearing.
Please submit your abstract by that date.
The program for the conference will be finalized and available on our website on Jan 23, 2006. Authors will also be informed individually by E-mail on the placement of their contribution in the program. Abstracts for oral and poster presentations are sollicited. Submission preferably through the conference website: http://focusonmicroscopy.org/ where also the conference registration is open and hotel booking information is available.
The earlier than usual deadline of 9 Jan. was chosen to give delegates sufficient time -after the program has been announced- to arrange their travel and acccommodation before the conference starting date of Sunday 9 April, 2006.
Please note that the conference will take place in the Esplanade Hotel in Fremantle, the historic waterfront suburb of Perth, See further our website.
The program will start on Sunday April 9, around 18 hours with an opening symposium in the Esplanade hotel followed by a welcome reception.
The conference Focuson Microscopy 2006 will take place as the next in a series of unique interdisciplinary meetings on advanced multidimensional light microscopy and image processing. The conference will be hosted by the University of Western Australia in Perth.
Focus on Microscopy 2006 is the continuation of a conference series presenting the latest innovations in optical microscopy and its applications in biology, medicine, material science, and information storage. 3D optical imaging and related theory are traditional subjects for the conference. The conference series is also known for covering the rapid development of advanced fluorescence labeling techniques for the confocal and multi-photon 3D imaging of -live- biological specimens. This year, in addition, special attention will be given to imaging in thick tissues.
Abstracts for contributions are invited and can now be submitted through the website:
www.FocusOnMicroscopy.org
where further information on the present and previous FOM conferences can be found.
Important dates:
Deadline for the submission of abstracts: January 9, 2006 Draft program available on the web: January 23, 2006 at website www.FocusOnMicroscopy.org Deadline for early registration: February 20, 2006
Welcoming you to beautiful Perth for the FOM2006 conference and exhibition.
With the best wishes for the New Year and on behalf of the organizing committee,
David Sampson, University of Western Australia, Perth, Australia
Fred Brakenhoff Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands
E-mail: info2006-at-FocusOnMicroscopy.org
Web: www.FocusOnMicroscopy.org
==============================Original Headers============================== 39, 30 -- From brakenho-at-science.uva.nl Tue Jan 3 02:18:50 2006 39, 30 -- Received: from imap.science.uva.nl (imap.science.uva.nl [146.50.4.51]) 39, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k038IluR006028 39, 30 -- for {microscopy-at-msa.microscopy.com} ; Tue, 3 Jan 2006 02:18:48 -0600 39, 30 -- Received: from webmail.science.uva.nl [146.50.4.91] 39, 30 -- by imap.science.uva.nl with ESMTP (sendmail 8.11.6p2/config 11.36). 39, 30 -- id k038Ij231650; Tue, 3 Jan 2006 09:18:46 +0100 39, 30 -- Received: from localhost 39, 30 -- by webmail.science.uva.nl (sendmail 8.11.6/config 11.35). 39, 30 -- id k038Ij232296; Tue, 3 Jan 2006 09:18:45 +0100 39, 30 -- X-Organisation: Faculty of Science, University of Amsterdam, The Netherlands 39, 30 -- X-URL: http://www.science.uva.nl/ 39, 30 -- Received: from localhost ([127.0.0.1]) 39, 30 -- (SquirrelMail authenticated user brakenho); 39, 30 -- by webmail.science.uva.nl with HTTP; 39, 30 -- Tue, 3 Jan 2006 09:18:45 +0100 (CET) 39, 30 -- Message-ID: {51496.84.254.54.91.1136276325.squirrel-at-84.254.54.91} 39, 30 -- Date: Tue, 3 Jan 2006 09:18:45 +0100 (CET) 39, 30 -- Subject: FOM2006 abstract deadline 9 Jan. near! FocusOnMicroscopy, Perth, 39, 30 -- April 9-14 2006 39, 30 -- From: "Fred Brakenhoff" {brakenho-at-science.uva.nl} 39, 30 -- To: microscopy-at-msa.microscopy.com 39, 30 -- User-Agent: SquirrelMail/1.4.3a 39, 30 -- X-Mailer: SquirrelMail/1.4.3a 39, 30 -- MIME-Version: 1.0 39, 30 -- Content-Type: text/plain;charset=iso-8859-1 39, 30 -- Content-Transfer-Encoding: 8bit 39, 30 -- X-Priority: 3 (Normal) 39, 30 -- Importance: Normal 39, 30 -- X-Virus-Scanned: by amavisd-new ==============================End of - Headers==============================
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Email: fisiltd-at-gmail.com Name: Howard Freeman
Organization: FISI Ltd
Title-Subject: [Filtered] O'Brien & McCully, Study of Plant Structure
Question: I have a copy of The study of Plant Structure, O'Brien & McCully, first printing, 1981 in very good condition. I used this for my teaching but have moved away from histology. If anyone is interested please send email to fisiltd-at-gmail.com
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Organization: pos-doc at INSA - Institut National des Sciences AppliquÈes de Lyon
Title-Subject: [Filtered] LM on 304L grey superficial layer and black lines
Question: I have shot peening 304L steel specimens, thickness about 5mm. Objective to visualise martensitic phase. I used tradional mechanical polishing, till 1200 grade, different etching based on water, HCl and potassium dissulfite (Berahas's tint). I did not clearly observe a martensitic layer but some insland, there is a dark grey to black layer on treated surface of about 10 microns, in case of highest resolution 200x I see it bright grey. What you believe it to be? I observe black flowing lines, variable lengths from 5 to 200 microns , paralel to surface through all specimen thickness and all its length. What it cab be? Some mention they are mechanical polishing effect others ferrite phase.
Thanks for whom took time reading or answering! Best new year wishes! Soraia Pirfo
Hi Sue, You give me an occasion to offer via the listserver some lore on MnO4 staining and epoxy resin adaptations to MnO4 staining that I provided off-listserver earlier this year in 2 other messages. I'll offer my message in three lengths.
SHORT VERSION is: Using epoxy embedding that excludes MNA (MNA?), MnO4 section staining followed by a lead stain (Sato's) can give fantastic contrast to everything --- not just membranes --- including any dirt/oil collected from the trough when picking up the sections, or rinsed off the pipette tip when dispensing drops of stain. The contrast is even greater if tannic acid has been used in the fix. Section staining of Epon-DDSA or Spurr embeddings is OK, but Araldite gives distinctly finer-grain higher-resolution staining.
MEDIUM VERSION: NMA renders use of KMnO4 as a section stain impossible; MnO4 reacts with the cured resin. Reedy, J Cell Biol 1965 26:309-11
We prefer the Mn-Pb sequence for higher contrast generally, and especially in the thinnest sections - 15-30 nm. So we gave up NMA about 30 years ago, and just heretically fiddled the resin-DDSA proportions until we got a hardness we liked; it worked just fine. With Epon that was that. We prefer Araldite because it is less even reactive than Epon or Spurr with MnO4. With Araldite-DDSA, we use Araldite 506. We found the best mixture still a bit too brittle, so added a bit of DER 736 as a "plasticizer", have used that for over 30 years. Araldite 506 50g 10g DDSA 75g 15g DER 736 10g 2g 1.8% v/v DMP-30 (range 1.5% to 2.0%). Reference Reedy et al (1983) J. Muscle Res. Cell Motil. 4:25-53.
LONG LONG VERSION (data dump): (This comes mostly from my off-listserver reply to some contributors to NetNotes in the May or June 2005 Microscopy today, regarding a query by Ursula J Potter about MnO4 use as a section stain.)
I have used 1-2% MnO4 section staining since 1964, almost exclusively since 1970, always followed by Pal's bleach (see below) to eliminate surface "pepper" of MnO2 ppt, followed then by a lead stain, of which Sato's appeals to us as the best and most storage-stable (Hanaichi et al, 1986, Proc 11th Internat'l EM Congress (Kyoto), p 2181). After publishing my brief note on the MNA problem (J Cell Biol, 1965, 26:309), I soon switched completely to Araldite-DDSA embedding, because it turned out that cured Araldite was much less reactive with MnO4 than any other epoxy resin mix I ever tested, including Spurr's when it later appeared. I used an extreme TEST, exposing cured blocks of various embedding resin mixtures to HOT KMnO4 solution (test tube immersed in boiling water) for 1 hr. In that test, the Epon-DDSA resin "preferred" in my Brief Note developed a blackened micro-fissured tree-bark surface (like that of MNA-Epon in unheated KMnO4, while the Araldite-DDSA resin was scarcely discolored at all. The Epon-DDSA sections typically presented a more nano-granular staining than did Araldite sections.
I think I gave up using barium MnO4 because it seemed to give blotchy staining more often than KMnO4. I also guessed that barium ion might capture carbonate as Pb did; Pb carbonate was the ubiquitous contaminant from hell with all the early lead stains that preceded lead citrate.
We wanted the maximum staining contrast that is provided by the Mn-Pb sequence because we mainly use 15-30 nm sections. Adding in UrAc to the sequence helps not at all. We do use UrAc as a block-stain, or as a secondary post-fixative (preferred to OsO4 in my lab) when our primary fixative is tannic acid with or (usually) without glutaraldehyde (TA-URAC works beautifully on myofilaments and on residual membranes in our permeabilized demembranated insect muscle, both in aqueous buffers at room temperature and as freeze-subst'n chemistry in acetone at -80 to -90°C). Recently, we have even found that the actin monomer substructure of thin myofilaments can be seen-- it appears to be relatively negatively stained, coated with metal derived from the TAURAC and Mn-Pb sequences (Fig 3, blue outlined: Fig 5 B; Liu et al, J Struct Biol, 2004, 147:268-282).
We had to experiment to find the optimum staining times for KMnO4, Pal's bleach washing, and lead stain needed to saturate contrast without "digesting" or extracting structures out of the sections. We learned by deliberately over-treating sections with each step. 60-90 min will remove Z-bands and (maybe) myofilaments from 100-150 nm Araldite sections, leaving a negative image, actually a cavity in the resin. Staining saturatees slower in Araldite than in Epon sections. 100 nm sections become stain-saturated slower than 25 nm sections. Cross-sections of muscle saturate much faster than longitudinal sections. 2-10 min in MnO4, 10-30s washing in Pal's bleach 1:100, and 1-5 min on Sato's Pb stain works for 25 nm longitudinal sections of muscle.
Stain apparently penetrates the section only where the pure embedding medium is modified by the presence of stained structures that intercept the section surface. The stains percolate only through the embedded structures, NOT through blank empty resin regions, as we found when sections had piled up or folded two-deep on one another; the covered section (sandwiched between carbon support film and an overlying surface section) would appear stained only within 0.1-0.2 um laterally from wherever the overlying section contained embedded structures such as filaments or membranes to conduct stain from the solution to the underlying structures in a covered section.
PAL's BLEACH, from the J. D. Robertson 1963 paper cited in my 1965 brief note: Distilled water 100 ml Potassium sulphite 0.5 g Oxalic acid 0.5 g dilute 1:100 for use (3-4 drops of above stock in 10 ml water), and run 10-20 drops over the grid after MnO4 staining. Water wash immediately after MnO4 and after Pal's.
All our staining is done by floating grids on small spherical drops of stain on fresh-washed Parafilm. (I'm not familiar with the vertical-insertion immersion staining of grids mentioned by Dr W. Muss in his helpful reply to you Dec 30.) Keys to clean staining: draw up the stains from under the surface in the stock bottles, discard the first 3-6 drops before putting the drop to be used down on a freshly washed Parafilm surface. Mistrust paranoically all unwashed surfaces on forceps and parafilm and pipette barrels as likely to have finger grease that will get on your sections or support films and become intensely stained contaminants. Wash everything you mistrust that will contact sections and grids with lavish flows and jets of deionized or distilled water. Stock lead stain needs replacing when it starts to "fails" in producing contrast, as often as every 4 weeks. Stock 2% KMnO4 lasts "forever"; just remember to respect and go below its MnO2-dirty surface when filling your pipette.
Around 1970 I switched to a lower-viscosity Araldite 506 mix (with DDSA, DER 732, DMP-30: see MEDIUM VERSION above for recipe and reference) that we have used ever since. It becomes as water-thin in viscosity as Spurr's when heated to 60°C, so we rotate vials on a small tilted platform in the oven for 20' x 3 changes to optimize infiltration, bypassing the 50:50 (etc) intermediate solvent-resin mixtures to go directly from solvent into 100% accelerated resin mix. We soon abandoned some of the other old-time religion as well, giving up propylene oxide completely to go directly from acetone or ethanol, after we tried deliberately curing 10-15 ml vials of accelerated resin with 10-20% solvent thoroughly blended in; the PO additive produced a much "cheesier" final cured resin than equivalent percentage of acetone or EtOH. (However, see my Dec 6 2005 on-listserver message RE: LR White contrast, calling attention to SW Brorson's use of propylene oxide and/or high [accelerator] to improve exposure of antigens in epoxy sections to immunolabeling). We also explored the epoxy-anhydride ratio over a wide and quite heretical range (30-70% v/v anhydride) to finally settle on a "nicer" final cured resin (less brittle during block-trimming than the gospel ratio) because we found acceptable cutting, staining, and structural preservation over the whole range we tried.
Some issues I consider still unfinished: 1) Can shorter or colder (etc) staining of Epon(replacement)-DDSA or Spurr sections eliminate the nano-granularity to approach the finer-grained stain we routinely get in Araldite? 2) Does our Mn-Pb section stain perhaps "clog" or "fill in" some fine structures in the 5 nm and smaller range that are more delicately and clearly brought out by the Ur-Pb staining sequence? I've yet to re-check my impression that the 4.8 nm axial repeat on thick filaments routinely showed up 30 years ago on optical diffraction of insect muscle section EMs, before we started using the MnPb exclusively; and that it never shows up now that we no longer use the weaker contrasting UrPb sequence.
-mike reedy-
At 9:28 PM -0600 12/29/05, susan.vanhorn-at-stonybrook.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
The easiest way to get a transmitted light image on the 510 is to just click on ChD, which is a transmitted light detector. This will collect all the light that passes through your sample AT THE SAME TIME you are collecting confocal fluorescent images. So no need to double-expose your sample. You will still need to set the condenser and neutral density filters, etc., for Koehler.
I am not sure if it is a bug that the HAL light goes off when scanning - I think it is probably a protection (safety) so the PMTs do not get blasted.
-Holly __________________ Holly L. Aaron CRL Molecular Imaging Center http://imaging.berkeley.edu
-----Original Message----- X-from: lubo-at-berkeley.edu [mailto:lubo-at-berkeley.edu] Sent: Wednesday, December 28, 2005 6:46 AM To: hollya-at-berkeley.edu
Thanks guys, I really apreciate it. I will try some of the mentioned methods and I will update you with the results and I will tell you which method(s) worked with me.
Best Regards
Hany
On 12/30/05, ramadanhany-at-gmail.com {ramadanhany-at-gmail.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Good morning, } } I grow tantalum oxide electrochemically on tantalum substrates, the } issue is that using SEM I can say that the surface of tantalum oxide } is full of defects "pin holes". I just use mechanical polishing of } tantalum before growing oxide, so I think that these defects result } from impurities of polishing sand papers. Is there any other way I can } polish tantalum to the finest grade avoiding these impurities? } } I appreciate your responses. } } Thanks } -- } ********************************************************** } Hany Ramadan } Graduate student } Chemistry department } McMaster university, Hamilton, Ontario, Canada } 905-525-9140 x: 26322 } elsayeh-at-mcmaster.ca } ********************************************************** } } } ==============================Original Headers============================== } 5, 23 -- From ramadanhany-at-gmail.com Fri Dec 30 10:10:44 2005 } 5, 23 -- Received: from zproxy.gmail.com (zproxy.gmail.com [64.233.162.201]) } 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBUGAiMs015284 } 5, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Dec 2005 10:10:44 -0600 } 5, 23 -- Received: by zproxy.gmail.com with SMTP id o37so2448119nzf } 5, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Dec 2005 08:10:44 -0800 (PST) } 5, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 5, 23 -- s=beta; d=gmail.com; } 5, 23 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 5, 23 -- b=PYN5psgGfgI0WLNifAGGkP00f9GUSHXDkWrIi+bDVwtHrgVUiPHtTSNzH8/qCj4iWo1PnzfQMBQZCsCEzGDhrrwV0seSVvIrg57GrNfpfp4NrW/hMtWkQ+jXcNmfpDmh6jp5Cvjb4kwfB1DUnbR47y1HRX3kvEdsyqmEtn0yvqE= } 5, 23 -- Received: by 10.65.231.8 with SMTP id i8mr4207763qbr; } 5, 23 -- Fri, 30 Dec 2005 08:10:44 -0800 (PST) } 5, 23 -- Received: by 10.65.156.18 with HTTP; Fri, 30 Dec 2005 08:10:44 -0800 (PST) } 5, 23 -- Message-ID: {8d8ce5a30512300810o3ce7e534tc4e1ac19820b4cd4-at-mail.gmail.com} } 5, 23 -- Date: Fri, 30 Dec 2005 11:10:44 -0500 } 5, 23 -- From: Hany Ramadan {ramadanhany-at-gmail.com} } 5, 23 -- To: Microscopy-at-microscopy.com } 5, 23 -- Subject: Tantalum polishing } 5, 23 -- MIME-Version: 1.0 } 5, 23 -- Content-Type: text/plain; charset=ISO-8859-1 } 5, 23 -- Content-Disposition: inline } 5, 23 -- Content-Transfer-Encoding: 8bit } 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jBUGAiMs015284 } ==============================End of - Headers============================== }
-- ********************************************************** Hany Ramadan Graduate student Chemistry department McMaster university, Hamilton, Ontario, Canada 905-525-9140 x: 26322 elsayeh-at-mcmaster.ca **********************************************************
==============================Original Headers============================== 7, 25 -- From ramadanhany-at-gmail.com Tue Jan 3 18:28:12 2006 7, 25 -- Received: from zproxy.gmail.com (zproxy.gmail.com [64.233.162.193]) 7, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k040SCVw032542 7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Jan 2006 18:28:12 -0600 7, 25 -- Received: by zproxy.gmail.com with SMTP id n29so4190029nzf 7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 03 Jan 2006 16:28:12 -0800 (PST) 7, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 25 -- s=beta; d=gmail.com; 7, 25 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 7, 25 -- b=jqvEITCtrHYGz22VW5v2TzDwTUPG6E7u8wxIz49Ct4T5ydsjuRU2DFVPLQemUrhx6KfdA71OlVgOpsJXvgrRMzHZ4Kd65rnXBquoj10M2tR4eawDB8YJdyC2xyIerEuZ1tzCcVsmxF6E6l/77PdQGmNx/sh55aJ32NRuNqyfplM= 7, 25 -- Received: by 10.65.177.14 with SMTP id e14mr4536714qbp; 7, 25 -- Tue, 03 Jan 2006 16:28:11 -0800 (PST) 7, 25 -- Received: by 10.65.156.18 with HTTP; Tue, 3 Jan 2006 16:28:11 -0800 (PST) 7, 25 -- Message-ID: {8d8ce5a30601031628o1cb70a8bre850d04cfd3b18d0-at-mail.gmail.com} 7, 25 -- Date: Tue, 3 Jan 2006 19:28:11 -0500 7, 25 -- From: Hany Ramadan {ramadanhany-at-gmail.com} 7, 25 -- To: Microscopy-at-microscopy.com 7, 25 -- Subject: Re: [Microscopy] Tantalum polishing 7, 25 -- In-Reply-To: {200512301648.jBUGmvLq022871-at-ns.microscopy.com} 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1 7, 25 -- Content-Disposition: inline 7, 25 -- References: {200512301648.jBUGmvLq022871-at-ns.microscopy.com} 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k040SCVw032542 ==============================End of - Headers==============================
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Title-Subject: [Filtered] SEMS annual meeting for 2006
Question: This year the annual meeting for the Southeastern Microscopy Society (SEMS) will be held jointly with the Association of Southeastern Biologists (ASB). The meeting will be March 29-April 1 in Gatlinburg, Tennessee at the Gatlinburg Convention Center. This event brings together approximately 800 biologists from across the southeastern United States. Interests are diverse, but range from genetics and molecular biology, to physiology and population biology, to community and ecosystem ecology.
Registration deadline is January 17, 2006 and information is available at the SEMS website:
http://www.semicroscopy.org/index.html or http://www.semicroscopy.org/meetings/call-for-papers.html
Cynthia S. Goldsmith Secretary, Southeastern Microscopy Society (SEMS) Mailstop G32 Centers for Disease Control and Prevention (CDC) Atlanta, GA 30333
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Title-Subject: [Filtered] Comparing cryo- and cryo-immuno diamond knives
Question: I would appreciate an advice from people who use cryo-immuno diamond knife from Diatome for the Tokuyasu technique. It is a newer model than their cryo diamond knife. Both can be seen at the following web site. http://www.emsdiasum.com/diatome/knife/immunocytochemistry.htm Is the newer model indeed better than the older one? Is there somebody in San Diego area who uses the cryo immuno knife? Thank you very much, Halina
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Email: rra-at-stowers-institute.org Name: Rhonda Allen
Organization: Stowers Institute
Title-Subject: [Filtered] glue to make ribbon's stick together
Question: Hello, I was wondering if any of you could tell me what kind of glue is used to make spurr's ribbon's stick together. I need to do serial sections and collect them all. Since I am new to this I am open for any and all suggestions. Thanks in adance. Rhonda Allen rra-at-stowers-institute.org
I seem to recall that diluted rubber cement can be used
rra-at-stowers-institute.org wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both rra-at-stowers-institute.org as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: rra-at-stowers-institute.org } Name: Rhonda Allen } } Organization: Stowers Institute } } Title-Subject: [Filtered] glue to make ribbon's stick together } } Question: Hello, } I was wondering if any of you could tell me what kind of glue is used to make spurr's ribbon's stick together. I need to do serial sections and collect them all. Since I am new to this I am open for any and all suggestions. } Thanks in adance. } Rhonda Allen } rra-at-stowers-institute.org } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 12 -- From zaluzec-at-microscopy.com Tue Jan 3 20:11:11 2006 } 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k042BADB010462 } 6, 12 -- for {microscopy-at-microscopy.com} ; Tue, 3 Jan 2006 20:11:10 -0600 } 6, 12 -- Mime-Version: 1.0 } 6, 12 -- X-Sender: (Unverified) } 6, 12 -- Message-Id: {p06110402bfe0df2a3482-at-[206.69.208.22]} } 6, 12 -- Date: Tue, 3 Jan 2006 20:11:08 -0600 } 6, 12 -- To: microscopy-at-microscopy.com } 6, 12 -- From: rra-at-stowers-institute.org (by way of MicroscopyListserver) } 6, 12 -- Subject: viaWWW: glue to make ribbon's stick together } 6, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
-- Greg Erdos 5410 SE 185th Ave. Micanopy, FL 32667
==============================Original Headers============================== 3, 24 -- From gwe-at-ufl.edu Wed Jan 4 16:03:58 2006 3, 24 -- Received: from smtp.ufl.edu (smtp02.osg.ufl.edu [128.227.74.165]) 3, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k04M3v0Z007902 3, 24 -- for {microscopy-at-microscopy.com} ; Wed, 4 Jan 2006 16:03:58 -0600 3, 24 -- Received: from [192.168.1.97] (adsl-152-56-36.gnv.bellsouth.net [70.152.56.36]) 3, 24 -- (authenticated bits=0) 3, 24 -- by smtp.ufl.edu (8.13.4/8.13.4/2.5.1) with ESMTP id k04M3tuh1663198; 3, 24 -- Wed, 4 Jan 2006 17:03:56 -0500 3, 24 -- Message-ID: {43BC4650.1010103-at-ufl.edu} 3, 24 -- Date: Wed, 04 Jan 2006 17:04:00 -0500 3, 24 -- From: greg erdos {gwe-at-ufl.edu} 3, 24 -- Reply-To: gwe-at-ufl.edu 3, 24 -- User-Agent: Thunderbird 1.5 (Windows/20051201) 3, 24 -- MIME-Version: 1.0 3, 24 -- To: rra-at-stowers-institute.org, microscopy-at-microscopy.com 3, 24 -- Subject: Re: [Microscopy] viaWWW: glue to make ribbon's stick together 3, 24 -- References: {200601040223.k042NquA014469-at-ns.microscopy.com} 3, 24 -- In-Reply-To: {200601040223.k042NquA014469-at-ns.microscopy.com} 3, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 24 -- Content-Transfer-Encoding: 7bit 3, 24 -- X-Spam-Status: hits=-1.524, required=5, tests=BAYES_01 3, 24 -- X-UFL-Spam-Status: hits=-1.524, required=5, tests=BAYES_01 3, 24 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 3, 24 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tsoumt-at-ilan.tpg.gov.tw as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tsoumt-at-ilan.tpg.gov.tw Name: Jack Tsou
Organization: I-lan Hospital, Taiwan
Title-Subject: [Filtered] Has anyone successfully mounted Canon pro1 onto trinocular port of Olympus BX51?
Question: I'm quite interested in the digital camera Canon pro1 and would like to know the detail if anybody had ever successfully mounted it onto the trinocular port of BX51. Thanks in advance!
If you haven't committed to the Canon Powershot Pro1 yet, I strongly recommend you move up to a camera with a removable lens like the Canon EOS 350D or The Nikon D50 - and use the Microscope as the lens.
On 5 Jan 2006, at 19:57, tsoumt-at-ilan.tpg.gov.tw wrote:
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } ---------------------------------------------------------------------- } ----- Remember this posting is most likely not from a Subscriber, so } when replying please copy both tsoumt-at-ilan.tpg.gov.tw as well as } the MIcroscopy Listserver } ---------------------------------------------------------------------- } ----- } } Email: tsoumt-at-ilan.tpg.gov.tw } Name: Jack Tsou } } Organization: I-lan Hospital, Taiwan } } Title-Subject: [Filtered] Has anyone successfully mounted Canon pro1 } onto trinocular port of Olympus BX51? } } Question: I'm quite interested in the digital camera Canon pro1 and } would like to know the detail if anybody had ever successfully mounted } it onto the trinocular port of BX51. Thanks in advance! } } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== 6, 12 -- From } zaluzec-at-microscopy.com Thu Jan 5 17:20:05 2006 6, 12 -- Received: } from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 6, 12 -- by } ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k05NJrdL005222 6, 12 } -- for {microscopy-at-microscopy.com} ; Thu, 5 Jan 2006 17:19:53 -0600 6, } 12 -- Mime-Version: 1.0 6, 12 -- X-Sender: (Unverified) 6, 12 -- } Message-Id: {p06110400bfe35a0001a2-at-[206.69.208.22]} 6, 12 -- Date: } Thu, 5 Jan 2006 17:19:47 -0600 6, 12 -- To: microscopy-at-microscopy.com } 6, 12 -- From: tsoumt-at-ilan.tpg.gov.tw (by way of MicroscopyListserver) } 6, 12 -- Subject: viaWWW: Canon pro1 onto trinocular port of Olympus } BX51 6, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"WE ARE MICROSOFT. RESISTANCE IS FUTILE. YOU WILL BE ASSIMILATED."
==============================Original Headers============================== 10, 25 -- From edelmare-at-muohio.edu Fri Jan 6 08:33:25 2006 10, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 10, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k06EXIIR006521 10, 25 -- for {microscopy-at-Microscopy.com} ; Fri, 6 Jan 2006 08:33:19 -0600 10, 25 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 10, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id k06EWWHB001896; 10, 25 -- Fri, 6 Jan 2006 09:32:32 -0500 10, 25 -- Received: from emf03 ([134.53.14.97]) 10, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id k06EWWSt016030; 10, 25 -- Fri, 6 Jan 2006 09:32:32 -0500 10, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 10, 25 -- To: tsoumt-at-ilan.tpg.gov.tw 10, 25 -- Date: Fri, 6 Jan 2006 09:33:29 -0500 10, 25 -- MIME-Version: 1.0 10, 25 -- Content-type: text/plain; charset=US-ASCII 10, 25 -- Content-transfer-encoding: 7BIT 10, 25 -- Subject: Re: [Microscopy] viaWWW: Canon pro1 onto trinocular port of Olympus BX51 10, 25 -- CC: microscopy-at-Microscopy.com 10, 25 -- Message-ID: {43BE3969.5908.ECA3945-at-localhost} 10, 25 -- Priority: normal 10, 25 -- In-reply-to: {200601060157.k061vebc022853-at-ns.microscopy.com} 10, 25 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 10, 25 -- X-Real-ConnectIP: 134.53.14.97 10, 25 -- X-Scanned-By: MIMEDefang 2.45 10, 25 -- X-Scanned-By: MIMEDefang 2.52 on 134.53.6.11 ==============================End of - Headers==============================
Hello everybody, One of our EM lab users wants to buy triangular tungsten carbide knives for serial sectioning. Is there any advantage in buying that kind of knife? What is a life span (how many blocks/sections can be cut before it becomes dull)? Can they be resharpen? Are they good for EM resins (Epon, Spurrs ect.)? Thanks Dorota
==============================Original Headers============================== 1, 22 -- From wadowska-at-avcn1.novell.upei.ca Fri Jan 6 11:00:05 2006 1, 22 -- Received: from mx.upei.ca (humboldt.cs.upei.ca [137.149.3.10]) 1, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k06H014x010343 1, 22 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jan 2006 11:00:02 -0600 1, 22 -- Received: from [137.149.64.250] (helo=acad1.cs.upei.ca) 1, 22 -- by mx.upei.ca with esmtp (Exim 3.35 #1 (Debian)) 1, 22 -- id 1EuuwX-00070Q-01 1, 22 -- for {microscopy-at-microscopy.com} ; Fri, 06 Jan 2006 12:59:57 -0400 1, 22 -- Received: from AVCN1/SpoolDir by acad1.cs.upei.ca (Mercury 1.48); 1, 22 -- 6 Jan 06 12:59:57 -0400 1, 22 -- Received: from SpoolDir by AVCN1 (Mercury 1.48); 6 Jan 06 12:59:36 -0400 1, 22 -- From: "Dorota Wadowska" {wadowska-at-upei.ca} 1, 22 -- Organization: University of P.E.I. 1, 22 -- To: microscopy-at-microscopy.com 1, 22 -- Date: Fri, 6 Jan 2006 12:10:46 -0400 1, 22 -- MIME-Version: 1.0 1, 22 -- Content-type: text/plain; charset=US-ASCII 1, 22 -- Content-transfer-encoding: 7BIT 1, 22 -- Subject: LM tungsten carbide knives 1, 22 -- Message-ID: {43BE5E46.13266.E4BB6B-at-localhost} 1, 22 -- Priority: normal 1, 22 -- X-mailer: Pegasus Mail for Win32 (v3.12c) ==============================End of - Headers==============================
For glue to get section ribbons to stick together, I like to use Weldwood Contact Cement (avialable at your local hardware store) diluted 1:1 in xylene. I picked up this tip somewhere a few years ago, but don't remember where. Maybe it was from Microscopy!
I assume you have trimed the block to be a 4 sided pyramid with a flat top being the blockface from which sections will be cut. To apply glue, use a small end of a toothpick, dip in diluted glue, and dab onto one of the 4 sides of the block face, either top or bottom relative to orientation of block during sectioning. I usually tilt the block face so that the side I choose is nearly horizontal so the glue doesn't run down the block side, or over the actual face of the block. Be careful so that glue does not get onto the blockface itself. Also, work quickly as the glue will dry fast. Then reorient the block for sectioning.
There is also a product out there called Tacky Wax, available from EM supply companies, which you dab onto the side of the block face. You may or may not need to apply gentle heat near it to get it to spread evenly over the side of the blockface. Perhaps others may wish to comment on the use of Tacky Wax.
Hope this helps you!
Gib ---- Gib Ahlstrand Imaging Center University of Minbnesota St. Paul, MN 55108 ahlst007-at-umn.eud http://www.cbs.umn.edu/ic
rra-at-stowers-institute.org wrote: } Email: rra-at-stowers-institute.org } Name: Rhonda Allen } } Organization: Stowers Institute } } Title-Subject: [Filtered] glue to make ribbon's stick together } } Question: Hello, } I was wondering if any of you could tell me what kind of glue is used to make spurr's ribbon's stick together. I need to do serial sections and collect them all. Since I am new to this I am open for any and all suggestions. } Thanks in adance. } Rhonda Allen } rra-at-stowers-institute.org
==============================Original Headers============================== 7, 20 -- From ahlst007-at-umn.edu Fri Jan 6 11:02:56 2006 7, 20 -- Received: from mtaout-m.tc.umn.edu (mtaout-m.tc.umn.edu [160.94.23.21]) 7, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k06H2oln010411 7, 20 -- for {Microscopy-at-Microscopy.com} ; Fri, 6 Jan 2006 11:02:55 -0600 7, 20 -- Received: from [160.94.39.28] (x160-94-39-28.cbs.umn.edu [160.94.39.28]) by mtaout-m.tc.umn.edu with ESMTP; Fri, 6 Jan 2006 11:02:44 -0600 (CST) 7, 20 -- X-Umn-Remote-Mta: [N] x160-94-39-28.cbs.umn.edu [160.94.39.28] #+LO+TS+AU+HN 7, 20 -- Message-ID: {43BEA2AF.7030808-at-umn.edu} 7, 20 -- Date: Fri, 06 Jan 2006 11:02:39 -0600 7, 20 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 7, 20 -- Reply-To: ahlst007-at-umn.edu 7, 20 -- Organization: Imaging Center UM 7, 20 -- User-Agent: Mozilla Thunderbird 1.0.7 (Macintosh/20050923) 7, 20 -- X-Accept-Language: en-us, en 7, 20 -- MIME-Version: 1.0 7, 20 -- To: rra-at-stowers-institute.org, Microscopy-at-Microscopy.com 7, 20 -- Subject: Re: [Microscopy] viaWWW: glue to make ribbon's stick together 7, 20 -- References: {200601040322.k043ML3t002256-at-ns.microscopy.com} 7, 20 -- In-Reply-To: {200601040322.k043ML3t002256-at-ns.microscopy.com} 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Job Opening #B005608; Lab Technician/Engineer at IBM
There is an opening at the Lab Technician/Engineer level within the Materials Characterization and Analysis Group in the Research Division of IBM at the Almaden Research Center in San Jose, California. The job is a long term (three years) supplemental position with benefits.This position involves technical support in an advanced electron microscopy laboratory which emphasizes transmission electron microscopy. The technician works as a team member with professional (Ph.D.) microscopists and other scientists in a research laboratory. Duties involve primarily sample preparation methods including the conventional grinding, polishing, and ion milling techniques, focused ion beam (FIB ) method, as well as Microtome, etc. In addition, the candidate must be able to operate and perform routine maintenance on specimen preparation equipment, interact with customers (professional scientists), and prepare reports. The candidate must be able to work independently within set priorities, to keep abreast of new advances, and to interact smoothly within a team.
Experience with sample preparation and basic TEM imaging is a plus.
IBM is an equal opportunity employer. Women and Minorities are encouraged to apply. Information on the Almaden Research Center can be found at www.almaden.ibm.com
Interested parties may call or send resumes to me at the following address:
Dr. Philip M. Rice IBM Research Division Almaden Research Center 650 Harry Road, K19B/D1 tel: (408) 927-1442 fax: (408) 927-2100 email: pmrice-at-almaden.ibm.com
==============================Original Headers============================== 10, 26 -- From pmrice-at-almaden.ibm.com Fri Jan 6 11:23:44 2006 10, 26 -- Received: from e2.ny.us.ibm.com (e2.ny.us.ibm.com [32.97.182.142]) 10, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k06HMhYt011005 10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jan 2006 11:23:10 -0600 10, 26 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234]) 10, 26 -- by e2.ny.us.ibm.com (8.12.11/8.12.11) with ESMTP id k06HMdHj029131 10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jan 2006 12:22:39 -0500 10, 26 -- Received: from d01av01.pok.ibm.com (d01av01.pok.ibm.com [9.56.224.215]) 10, 26 -- by d01relay02.pok.ibm.com (8.12.10/NCO/VERS6.8) with ESMTP id k06HMd87122936 10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jan 2006 12:22:39 -0500 10, 26 -- Received: from d01av01.pok.ibm.com (loopback [127.0.0.1]) 10, 26 -- by d01av01.pok.ibm.com (8.12.11/8.13.3) with ESMTP id k06HMdBn004984 10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jan 2006 12:22:39 -0500 10, 26 -- Received: from d01ml605.pok.ibm.com (d01ml605.pok.ibm.com [9.56.227.91]) 10, 26 -- by d01av01.pok.ibm.com (8.12.11/8.12.11) with ESMTP id k06HMck9004981 10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jan 2006 12:22:38 -0500 10, 26 -- Subject: TEM - Job Opening for TEM Specimen Preparation Lab Technician 10, 26 -- To: microscopy-at-microscopy.com 10, 26 -- X-Mailer: Lotus Notes Release 6.0.2CF1 June 9, 2003 10, 26 -- Message-ID: {OFBC79C14C.36D1DACC-ON882570EE.005F03ED-882570EE.005F72CD-at-us.ibm.com} 10, 26 -- From: Philip Rice {pmrice-at-almaden.ibm.com} 10, 26 -- Date: Fri, 6 Jan 2006 09:22:33 -0800 10, 26 -- X-MIMETrack: Serialize by Router on D01ML605/01/M/IBM(Release 7.0HF90 | November 16, 2005) at 10, 26 -- 01/06/2006 12:22:38 10, 26 -- MIME-Version: 1.0 10, 26 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gsosinsky-at-ucsd.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gsosinsky-at-ucsd.edu Name: Gina Sosinsky
Organization: University of California, San Diego
Title-Subject: [Filtered] 4th International Congress for Electron Tomography 2006
Question: **************** First Announcement *************** The Fourth International Congress on Electron Tomography, (4ICET), will be held at Paradise Point, San Diego November 5-8, 2006.
This congress brings together biologists, biophysicists, computer scientists, mathematicians, materials scientists and electron optical instrumentation specialists for an interdisciplinary exchange of ideas focusing on advancing methods of electron tomography (ET) in biology. ET has moved from a specialized experimental technique practiced by a few laboratories to one delivering critical information to a broad community of cell biologists, structural biologists, and neuroscientists. For students, this conference presents a unique opportunity to learn about cutting-edge advances in ET applications and methodologies.
Sessions will include:
ï Imaging of dynamic structures and correlative microscopy Co-Chairs: O. Medalia (Ben-Gurion Univ.); Jack Johnson (The Scripps Research Institute)
ï Advances in instrumentation Co-chairs: M. Ellisman (UCSD); Bram Koster (Univ. ofUltrect, Netherlands)
ï 3D reconstruction algorithms Co-chairs: N. Volkmann (Burnham Institute); TBA
ï Visualization & quantitative analysis Co-chairs: R. Whitaker (Univ. of Utah); D. Mastronarde (Univ. of Colorado)
ï Moving tomography to the mainstream: data sharing, data integration, & model building Co-chairs: M. Martone (UCSD); J-M. Carazo (Univ. of Madrid)
ï Emerging technologies for the multiscale: cell to tissue and molecule to cell Co-chairs: D. Hanein (Burnham Institute); C. Larabell (UCSF)
Sessions will include both invited speakers and talks selected from submitted abstracts.
For further information please check out our web site: http://www.4icet.org
or contact Mark H. Ellisman, Lead Congress Organizer (mark-at-ncmir.ucsd.edu) or Grace Osborne, Congress Coordinator (gosborne-at-ncmir,ucsd.edu)
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gsosinsky-at-ucsd.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gsosinsky-at-ucsd.edu Name: Gina Sosinsky
Organization: University of California, San Diego
Title-Subject: [Filtered] 4th International Congress for Electron Tomography 2006
Question: **************** First Announcement *************** The Fourth International Congress on Electron Tomography, (4ICET), will be held at Paradise Point, San Diego November 5-8, 2006.
This congress brings together biologists, biophysicists, computer scientists, mathematicians, materials scientists and electron optical instrumentation specialists for an interdisciplinary exchange of ideas focusing on advancing methods of electron tomography (ET) in biology. ET has moved from a specialized experimental technique practiced by a few laboratories to one delivering critical information to a broad community of cell biologists, structural biologists, and neuroscientists. For students, this conference presents a unique opportunity to learn about cutting-edge advances in ET applications and methodologies.
Sessions will include:
ï Imaging of dynamic structures and correlative microscopy Co-Chairs: O. Medalia (Ben-Gurion Univ.); Jack Johnson (The Scripps Research Institute)
ï Advances in instrumentation Co-chairs: M. Ellisman (UCSD); Bram Koster (Univ. ofUltrect, Netherlands)
ï 3D reconstruction algorithms Co-chairs: N. Volkmann (Burnham Institute); TBA
ï Visualization & quantitative analysis Co-chairs: R. Whitaker (Univ. of Utah); D. Mastronarde (Univ. of Colorado)
ï Moving tomography to the mainstream: data sharing, data integration, & model building Co-chairs: M. Martone (UCSD); J-M. Carazo (Univ. of Madrid)
ï Emerging technologies for the multiscale: cell to tissue and molecule to cell Co-chairs: D. Hanein (Burnham Institute); C. Larabell (UCSF)
Sessions will include both invited speakers and talks selected from submitted abstracts.
For further information please check out our web site: http://www.4icet.org
or contact Mark H. Ellisman, Lead Congress Organizer (mark-at-ncmir.ucsd.edu) or Grace Osborne, Congress Coordinator (gosborne-at-ncmir,ucsd.edu)
Year 10 was last year: I hope you can join us as the UBC Course enters its second decade.
Speaking for myself, and I am sure for our faculty, it is a source of great satisfaction to see how many of the contributors to this list are UBC Course Alumnae.
Thank you all.
Jim P.
Eleventh Annual INTERNATIONAL 12-Day Short Course on 3D Microscopy of Living Cells, June 10 - 22, 2006 (Pre-course: June 10)
Tenth, Post-course Workshop on 3D Image Processing, June 24 -26, 2006
Organized by Prof. James Pawley, (University of Wisconsin-Madison)
in association with the Departments of Pharmacology and Physiology and the Brain Research Centre, University of British Columbia, Vancouver, BC, Canada
DATES
Applications must be received by Tuesday, March 15, 2006 Deposit due Friday, April 15, 2006 Registration 5:00 - 7:00 PM Saturday, June 10, 2006 First Lecture 7:30 PM Saturday, June 10, 2006 Live-cell Course ends, noon Thursday, June 22, 2006 3D Image Processing Course, Saturday, June 24 - Monday, 26, 2006
APPLICATIONS DUE BY MARCH 15, 2006
APPLICATIONS Applicants must complete a questionnaire on the web to assess knowledge level, field of interest and proposed personal, live-cell, project. But don't let this put you off: if you plan to use 3D microscopy on living cells, we can usually find a way to make it work.
Enrollment will be limited to about 32 participants (exact number depends on number of 3D Systems available). Selection will be made on the basis of background and perceived need. Those without previous LM experience will be provided with access to basic texts to read before the course begins. Application forms may be down-loaded from the WWW site at
http://www.3dcourse.ubc.ca/application.htm , or obtained from:
Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 250 N. Mills Street, Madison, WI, 53706 JBPAWLEY-at-WISC.EDU
Additional information is available from: http://www.3dcourse.ubc.ca/brochure.htm and links.
We expect to have at least 11, 3D microscope workstations for student use and there will be an international faculty of 20.
Application deadlines:
Application forms should be received for screening by March 15, 2006. Successful applicants will be notified by April 1, and a deposit of 50% must be received by April 15, 2006. In general, refunds of the deposit will only be possible if someone on the waiting list can take the place but this has not been a problem in previous years. The remaining balance is due before Registration.
Pre-Course Tuition (1/2 day Basic Optical principles) $125 (US) 3D Live-cell Course Tuition (includes lunches, snacks, 3 dinners, incl. the NEW Third Edition of the Handbook of Biological Confocal Microscopy): $2,750 (US) Workshop Tuition (includes lunches and snacks): $1,100 (US)
Room/board about $40/day (US) depending on room type.
I hope that this includes all of the information that you need, but if not, please get back to me.
Jim Pawley
-- **************************************** Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-262-9083 250 N. Mills St., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU "A scientist is not one who can answer questions but one who can question answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39
==============================Original Headers============================== 27, 24 -- From jbpawley-at-wisc.edu Fri Jan 6 12:54:20 2006 27, 24 -- Received: from smtp4.wiscmail.wisc.edu (hermes.doit.wisc.edu [144.92.197.190]) 27, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k06IrMoO013779 27, 24 -- for {Microscopy-at-Microscopy.Com} ; Fri, 6 Jan 2006 12:53:44 -0600 27, 24 -- Received: from avs-daemon.smtp4.wiscmail.wisc.edu by smtp4.wiscmail.wisc.edu 27, 24 -- (iPlanet Messaging Server 5.2 HotFix 2.08 (built Sep 22 2005)) 27, 24 -- id {0ISO0090SPSXW6-at-smtp4.wiscmail.wisc.edu} for Microscopy-at-Microscopy.Com; 27, 24 -- Fri, 06 Jan 2006 12:53:21 -0600 (CST) 27, 24 -- Received: from [144.92.238.207] by smtp4.wiscmail.wisc.edu 27, 24 -- (iPlanet Messaging Server 5.2 HotFix 2.08 (built Sep 22 2005)) 27, 24 -- with ESMTPSA id {0ISO00LB4PSRMA-at-smtp4.wiscmail.wisc.edu} for 27, 24 -- Microscopy-at-Microscopy.Com; Fri, 06 Jan 2006 12:53:16 -0600 (CST) 27, 24 -- Date: Fri, 06 Jan 2006 12:53:14 -0600 27, 24 -- From: James Pawley {jbpawley-at-wisc.edu} 27, 24 -- Subject: First announcement: Eleventh International UBC Live-cell Course. 27, 24 -- X-Sender: jbpawley-at-wiscmail.wisc.edu 27, 24 -- To: "ListServer-at-MSA.Microscopy.Com" {Microscopy-at-Microscopy.Com} 27, 24 -- Message-id: {p06110415bfe46d18adc1-at-[144.92.238.207]} 27, 24 -- MIME-version: 1.0 27, 24 -- Content-type: text/plain; format=flowed; charset=us-ascii 27, 24 -- Content-transfer-encoding: 7BIT 27, 24 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=0.0.0.0 27, 24 -- X-Spam-PmxInfo: Server=avs-8, Version=5.1.1.222179, Antispam-Engine: 2.1.0.0, 27, 24 -- Antispam-Data: 2006.1.6.19, SenderIP=0.0.0.0 ==============================End of - Headers==============================
In our lab, there was a person using a thin coat of dental wax successfully on bottom side of a block. Melt dental in a beaker and apply a tiny bit to a shaped block with a warm spatula. You may have to trim out excess wax.
I have Tackiwax also. It came in a tiny bottle. It seems to me that it is repackaged dental wax without color. On the other hand, I may be wrong this material.
Ann Fook Yang EM Unit/ Unite EM AAFC/AAC 960 Carling Ave, Ottawa,Ontario Canada K1A 0C6 yanga-at-agr.gc.ca Telephone/Téléphone: 613-759-1638 Facsimile/Télécopieur: 613-759-1701
Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada
-----Original Message----- X-from: ahlst007-at-umn.edu [mailto:ahlst007-at-umn.edu] Sent: Friday, January 06, 2006 5:35 PM To: Yang, Ann-Fook
Rhonda,
For glue to get section ribbons to stick together, I like to use Weldwood Contact Cement (avialable at your local hardware store) diluted 1:1 in xylene. I picked up this tip somewhere a few years ago, but don't remember where. Maybe it was from Microscopy!
I assume you have trimed the block to be a 4 sided pyramid with a flat top being the blockface from which sections will be cut. To apply glue, use a small end of a toothpick, dip in diluted glue, and dab onto one of the 4 sides of the block face, either top or bottom relative to orientation of block during sectioning. I usually tilt the block face so that the side I choose is nearly horizontal so the glue doesn't run down the block side, or over the actual face of the block. Be careful so that glue does not get onto the blockface itself. Also, work quickly as the glue will dry fast. Then reorient the block for sectioning.
There is also a product out there called Tacky Wax, available from EM supply companies, which you dab onto the side of the block face. You may or may not need to apply gentle heat near it to get it to spread evenly over the side of the blockface. Perhaps others may wish to comment on the use of Tacky Wax.
Hope this helps you!
Gib ---- Gib Ahlstrand Imaging Center University of Minbnesota St. Paul, MN 55108 ahlst007-at-umn.eud http://www.cbs.umn.edu/ic
rra-at-stowers-institute.org wrote: } Email: rra-at-stowers-institute.org } Name: Rhonda Allen } } Organization: Stowers Institute } } Title-Subject: [Filtered] glue to make ribbon's stick together } } Question: Hello, } I was wondering if any of you could tell me what kind of glue is used to make spurr's ribbon's stick together. I need to do serial sections and collect them all. Since I am new to this I am open for any and all suggestions. } Thanks in adance. } Rhonda Allen } rra-at-stowers-institute.org
==============================Original Headers============================== 7, 20 -- From ahlst007-at-umn.edu Fri Jan 6 11:02:56 2006 7, 20 -- Received: from mtaout-m.tc.umn.edu (mtaout-m.tc.umn.edu [160.94.23.21]) 7, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k06H2oln010411 7, 20 -- for {Microscopy-at-Microscopy.com} ; Fri, 6 Jan 2006 11:02:55 -0600 7, 20 -- Received: from [160.94.39.28] (x160-94-39-28.cbs.umn.edu [160.94.39.28]) by mtaout-m.tc.umn.edu with ESMTP; Fri, 6 Jan 2006 11:02:44 -0600 (CST) 7, 20 -- X-Umn-Remote-Mta: [N] x160-94-39-28.cbs.umn.edu [160.94.39.28] #+LO+TS+AU+HN 7, 20 -- Message-ID: {43BEA2AF.7030808-at-umn.edu} 7, 20 -- Date: Fri, 06 Jan 2006 11:02:39 -0600 7, 20 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 7, 20 -- Reply-To: ahlst007-at-umn.edu 7, 20 -- Organization: Imaging Center UM 7, 20 -- User-Agent: Mozilla Thunderbird 1.0.7 (Macintosh/20050923) 7, 20 -- X-Accept-Language: en-us, en 7, 20 -- MIME-Version: 1.0 7, 20 -- To: rra-at-stowers-institute.org, Microscopy-at-Microscopy.com 7, 20 -- Subject: Re: [Microscopy] viaWWW: glue to make ribbon's stick together 7, 20 -- References: {200601040322.k043ML3t002256-at-ns.microscopy.com} 7, 20 -- In-Reply-To: {200601040322.k043ML3t002256-at-ns.microscopy.com} 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 16, 29 -- From YANGA-at-AGR.GC.CA Mon Jan 9 09:25:19 2006 16, 29 -- Received: from agroutsmtp1.agr.gc.ca (agroutsmtp1.agr.gc.ca [192.197.71.175]) 16, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k09FPHkv029897 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 09:25:18 -0600 16, 29 -- Received: from agrin1-old.agr.gc.ca (agrgate.agr.ca [192.197.71.189]) 16, 29 -- by agroutsmtp1.agr.gc.ca (8.12.8/8.12.8) with ESMTP id k09FPDHp017974 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 10:25:13 -0500 16, 29 -- Received: from onncrxcn1.AGR.GC.CA ([10.117.15.130]) 16, 29 -- by agrin1-old.agr.gc.ca (8.12.8/8.12.8) with ESMTP id k09FQiUY023252 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 10:26:45 -0500 16, 29 -- Received: from onncrxms3.AGR.GC.CA ([10.117.15.30]) by onncrxcn1.AGR.GC.CA with Microsoft SMTPSVC(5.0.2195.6713); 16, 29 -- Mon, 9 Jan 2006 10:25:08 -0500 16, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 16, 29 -- content-class: urn:content-classes:message 16, 29 -- MIME-Version: 1.0 16, 29 -- Content-Type: text/plain; 16, 29 -- charset="iso-8859-1" 16, 29 -- Subject: RE: [Microscopy] glue to make ribbon's stick together 16, 29 -- Date: Mon, 9 Jan 2006 10:25:06 -0500 16, 29 -- Message-ID: {E035A9C87303AE4AB9BA10FD8324DFB10243345A-at-onncrxms3.agr.gc.ca} 16, 29 -- X-MS-Has-Attach: 16, 29 -- X-MS-TNEF-Correlator: 16, 29 -- Thread-Topic: [Microscopy] glue to make ribbon's stick together 16, 29 -- Thread-Index: AcYT2iOC0AiPsPOzQuGBu5lpBYTxtgBUcMiw 16, 29 -- From: "Yang, Ann-Fook" {YANGA-at-AGR.GC.CA} 16, 29 -- To: {microscopy-at-microscopy.com} 16, 29 -- X-OriginalArrivalTime: 09 Jan 2006 15:25:08.0115 (UTC) FILETIME=[DFA16E30:01C61530] 16, 29 -- Content-Transfer-Encoding: 8bit 16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k09FPHkv029897 ==============================End of - Headers==============================
Does anyone know if the various glues suggested vaporise in the TEM? If so does this cause any problems?
Dave
-----Original Message----- X-from: YANGA-at-AGR.GC.CA [mailto:YANGA-at-AGR.GC.CA] Sent: 09 January 2006 15:28 To: David Patton
In our lab, there was a person using a thin coat of dental wax successfully on bottom side of a block. Melt dental in a beaker and apply a tiny bit to a shaped block with a warm spatula. You may have to trim out excess wax.
I have Tackiwax also. It came in a tiny bottle. It seems to me that it is repackaged dental wax without color. On the other hand, I may be wrong this material.
Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada
-----Original Message----- X-from: ahlst007-at-umn.edu [mailto:ahlst007-at-umn.edu] Sent: Friday, January 06, 2006 5:35 PM To: Yang, Ann-Fook
Rhonda,
For glue to get section ribbons to stick together, I like to use Weldwood Contact Cement (avialable at your local hardware store) diluted 1:1 in xylene. I picked up this tip somewhere a few years ago, but don't remember where. Maybe it was from Microscopy!
I assume you have trimed the block to be a 4 sided pyramid with a flat top being the blockface from which sections will be cut. To apply glue, use a small end of a toothpick, dip in diluted glue, and dab onto one of the 4 sides of the block face, either top or bottom relative to orientation of block during sectioning. I usually tilt the block face so that the side I choose is nearly horizontal so the glue doesn't run down the block side, or over the actual face of the block. Be careful so that glue does not get onto the blockface itself. Also, work quickly as the glue will dry fast. Then reorient the block for sectioning.
There is also a product out there called Tacky Wax, available from EM supply companies, which you dab onto the side of the block face. You may or may not need to apply gentle heat near it to get it to spread evenly over the side of the blockface. Perhaps others may wish to comment on the use of Tacky Wax.
Hope this helps you!
Gib ---- Gib Ahlstrand Imaging Center University of Minbnesota St. Paul, MN 55108 ahlst007-at-umn.eud http://www.cbs.umn.edu/ic
rra-at-stowers-institute.org wrote: } Email: rra-at-stowers-institute.org } Name: Rhonda Allen } } Organization: Stowers Institute } } Title-Subject: [Filtered] glue to make ribbon's stick together } } Question: Hello, } I was wondering if any of you could tell me what kind of glue is used to make spurr's ribbon's stick together. I need to do serial sections and collect them all. Since I am new to this I am open for any and all suggestions. } Thanks in adance. } Rhonda Allen } rra-at-stowers-institute.org
==============================Original Headers============================== 7, 20 -- From ahlst007-at-umn.edu Fri Jan 6 11:02:56 2006 7, 20 -- Received: from mtaout-m.tc.umn.edu (mtaout-m.tc.umn.edu [160.94.23.21]) 7, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k06H2oln010411 7, 20 -- for {Microscopy-at-Microscopy.com} ; Fri, 6 Jan 2006 11:02:55 -0600 7, 20 -- Received: from [160.94.39.28] (x160-94-39-28.cbs.umn.edu [160.94.39.28]) by mtaout-m.tc.umn.edu with ESMTP; Fri, 6 Jan 2006 11:02:44 -0600 (CST) 7, 20 -- X-Umn-Remote-Mta: [N] x160-94-39-28.cbs.umn.edu [160.94.39.28] #+LO+TS+AU+HN 7, 20 -- Message-ID: {43BEA2AF.7030808-at-umn.edu} 7, 20 -- Date: Fri, 06 Jan 2006 11:02:39 -0600 7, 20 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 7, 20 -- Reply-To: ahlst007-at-umn.edu 7, 20 -- Organization: Imaging Center UM 7, 20 -- User-Agent: Mozilla Thunderbird 1.0.7 (Macintosh/20050923) 7, 20 -- X-Accept-Language: en-us, en 7, 20 -- MIME-Version: 1.0 7, 20 -- To: rra-at-stowers-institute.org, Microscopy-at-Microscopy.com 7, 20 -- Subject: Re: [Microscopy] viaWWW: glue to make ribbon's stick together 7, 20 -- References: {200601040322.k043ML3t002256-at-ns.microscopy.com} 7, 20 -- In-Reply-To: {200601040322.k043ML3t002256-at-ns.microscopy.com} 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 16, 29 -- From YANGA-at-AGR.GC.CA Mon Jan 9 09:25:19 2006 16, 29 -- Received: from agroutsmtp1.agr.gc.ca (agroutsmtp1.agr.gc.ca [192.197.71.175]) 16, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k09FPHkv029897 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 09:25:18 -0600 16, 29 -- Received: from agrin1-old.agr.gc.ca (agrgate.agr.ca [192.197.71.189]) 16, 29 -- by agroutsmtp1.agr.gc.ca (8.12.8/8.12.8) with ESMTP id k09FPDHp017974 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 10:25:13 -0500 16, 29 -- Received: from onncrxcn1.AGR.GC.CA ([10.117.15.130]) 16, 29 -- by agrin1-old.agr.gc.ca (8.12.8/8.12.8) with ESMTP id k09FQiUY023252 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 10:26:45 -0500 16, 29 -- Received: from onncrxms3.AGR.GC.CA ([10.117.15.30]) by onncrxcn1.AGR.GC.CA with Microsoft SMTPSVC(5.0.2195.6713); 16, 29 -- Mon, 9 Jan 2006 10:25:08 -0500 16, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 16, 29 -- content-class: urn:content-classes:message 16, 29 -- MIME-Version: 1.0 16, 29 -- Content-Type: text/plain; 16, 29 -- charset="iso-8859-1" 16, 29 -- Subject: RE: [Microscopy] glue to make ribbon's stick together 16, 29 -- Date: Mon, 9 Jan 2006 10:25:06 -0500 16, 29 -- Message-ID: {E035A9C87303AE4AB9BA10FD8324DFB10243345A-at-onncrxms3.agr.gc.ca} 16, 29 -- X-MS-Has-Attach: 16, 29 -- X-MS-TNEF-Correlator: 16, 29 -- Thread-Topic: [Microscopy] glue to make ribbon's stick together 16, 29 -- Thread-Index: AcYT2iOC0AiPsPOzQuGBu5lpBYTxtgBUcMiw 16, 29 -- From: "Yang, Ann-Fook" {YANGA-at-AGR.GC.CA} 16, 29 -- To: {microscopy-at-microscopy.com} 16, 29 -- X-OriginalArrivalTime: 09 Jan 2006 15:25:08.0115 (UTC) FILETIME=[DFA16E30:01C61530] 16, 29 -- Content-Transfer-Encoding: 8bit 16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k09FPHkv029897 ==============================End of - Headers==============================
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==============================Original Headers============================== 29, 33 -- From David.Patton-at-uwe.ac.uk Mon Jan 9 09:32:45 2006 29, 33 -- Received: from mailapp02.uwe.ac.uk (mailapp02.uwe.ac.uk [164.11.132.63]) 29, 33 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k09FWhRa002940 29, 33 -- for {Microscopy-at-Microscopy.com} ; Mon, 9 Jan 2006 09:32:44 -0600 29, 33 -- Received: from (164.11.132.62) by mailapp02.uwe.ac.uk via smtp 29, 33 -- id 0461_8549066c_8125_11da_8b14_0002b3c90020; 29, 33 -- Mon, 09 Jan 2006 15:35:11 +0000 29, 33 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 29, 33 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121]) 29, 33 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 29, 33 -- 2005)) with ESMTP id {0ISU00H0T0IHDB-at-mta02.uwe.ac.uk} for 29, 33 -- Microscopy-at-Microscopy.com; Mon, 09 Jan 2006 15:32:41 +0000 (GMT) 29, 33 -- Date: Mon, 09 Jan 2006 15:32:40 +0000 29, 33 -- From: David Patton {David.Patton-at-uwe.ac.uk} 29, 33 -- Subject: RE: [Microscopy] RE: glue to make ribbon's stick together 29, 33 -- To: Microscopy-at-Microscopy.com 29, 33 -- Message-id: {F247F674896BE243AD8263C5280E2BDBF8507D-at-egen-uwe01} 29, 33 -- MIME-version: 1.0 29, 33 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5.6944.0 29, 33 -- Content-type: text/plain; 29, 33 -- charset="utf-8" 29, 33 -- Content-class: urn:content-classes:message 29, 33 -- Thread-topic: [Microscopy] RE: glue to make ribbon's stick together 29, 33 -- Thread-index: AcYVMVNhlhbSoF3kQxeNI9avIx8RPAAAF3QQ 29, 33 -- X-MS-Has-Attach: 29, 33 -- X-MS-TNEF-Correlator: 29, 33 -- X-NAI-Spam-Score: -0.3 29, 33 -- X-NAI-Spam-Rules: 1 Rules triggered 29, 33 -- BAYES_30=-0.3 29, 33 -- X-NAIMIME-Disclaimer: 1 29, 33 -- X-NAIMIME-Modified: 1 29, 33 -- Content-Transfer-Encoding: 8bit 29, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k09FWhRa002940 ==============================End of - Headers==============================
I posted this question a couple of weeks ago, but probably the timing was wrong (just before the holidays), no answers at all. So I am posting again:
I would need a tip on a good (commercially available) antibody against His tag for EM - Tokuyasu technique. It should tolerate at least light (0.1-0.2%) glutaraldehyde fixation. A rabbit polyclonal would be preferable, but a working monoclonal would do as well.
Another unrelated question - I would need to label macrophages in sections (again, Tokuyasu). Any idea for a good macrophage marker?
Thanks,
Michal
==============================Original Headers============================== 7, 19 -- From M_Jarnik-at-fccc.edu Mon Jan 9 10:03:32 2006 7, 19 -- Received: from azah.fccc.edu (azah.fccc.edu [131.249.4.237]) 7, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k09G3VhJ016139 7, 19 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 10:03:32 -0600 7, 19 -- Received: from fccc.edu (emf4.fccc.edu [131.249.9.170]) 7, 19 -- by azah.fccc.edu (8.12.11/8.12.11) with ESMTP id k09G3USd021508 7, 19 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 11:03:30 -0500 (EST) 7, 19 -- Message-ID: {43C28952.4040809-at-fccc.edu} 7, 19 -- Date: Mon, 09 Jan 2006 11:03:30 -0500 7, 19 -- From: Michal Jarnik {M_Jarnik-at-fccc.edu} 7, 19 -- Reply-To: M_Jarnik-at-fccc.edu 7, 19 -- Organization: Fox Chase Cancer Center 7, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 7, 19 -- X-Accept-Language: en,cs 7, 19 -- MIME-Version: 1.0 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- Subject: His Tag antibody for EM 7, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 7, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mjo10-at-psu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mjo10-at-psu.edu Name: Matthew Olszta
Organization: Penn State University
Title-Subject: [Filtered] Vanadium oxide thin films
Question: I wanted to know if anyone has had any experience with TEM sample preparation and analysis of vanadium oxide thin films. The reason I ask is that certain vanadium oxide crystal structures (VO2 and V2O5) are soluble or slightly soluble in water, but using water in polishing I have not yet seen any appreciable dissolution in my cross-sections.
If others have worked with these materials, or other water sensitive materials, do you have any suggestions or comments on sample preparation in order to avoid artifacts?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mjo10-at-psu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mjo10-at-psu.edu Name: Matthew Olszta
Organization: Penn State University
Title-Subject: [Filtered] Vanadium oxide thin films
Question: I wanted to know if anyone has had any experience with TEM sample preparation and analysis of vanadium oxide thin films. The reason I ask is that certain vanadium oxide crystal structures (VO2 and V2O5) are soluble or slightly soluble in water, but using water in polishing I have not yet seen any appreciable dissolution in my cross-sections.
If others have worked with these materials, or other water sensitive materials, do you have any suggestions or comments on sample preparation in order to avoid artifacts?
I am not sure about vaporization issues, but I have used another glue. Using a sharpened toothpick dipped in acetone, I have dissolved glue from 3M adhesive tape ("Magic Tape") to apply thin amounts of glue to the top surface of the block when trying to obtain serial sections. I have not noted any contamination problems, but clearly you want to refrain from getting any of this solvent on the cutting face.
However, when collecting serial sections, glue should only be your last resort. When properly trimmed with a fresh razor blade (I like the chromium steel blades from "Wilkinson Sword Blades") so that both top edge and bottom edge are parallel to each other and to the diamond knife edge, most Epon-like resins allow serial sections to stick together quite well. I don't know how well this applies to Spurr's in particular, but I expect the same results. The cleaner those edges are, and the more parallel they are to each other and to the diamond, the better the result. Acrylic resins like LR White or LR Gold tend to be much more brittle, and those would be more difficult to handle in series.
Dave Hall
For a more exhaustive discussion of serial sectioning, see Hall (1995) Methods in Cell Biology, C. elegans: Modern Biological Analysis of an Organism. Epstein and Shakes (eds) Academic Press. pp. 395-436. -- David H. Hall, Ph.D. Center for C. elegans Anatomy Department of Neuroscience Albert Einstein College of Medicine 1410 Pelham Parkway Bronx, NY 10461
www.wormatlas.org www.aecom.yu.edu/wormem
phone 718 430-2195 fax 718 430-2514
==============================Original Headers============================== 6, 21 -- From hall-at-aecom.yu.edu Tue Jan 10 10:06:01 2006 6, 21 -- Received: from mailgw.aecom.yu.edu (mailgw.aecom.yu.edu [129.98.1.16]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0AG61X7031584 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2006 10:06:01 -0600 6, 21 -- Received: from mailvx.aecom.yu.edu (mailvx.aecom.yu.edu [129.98.1.17]) 6, 21 -- by mailgw.aecom.yu.edu (8.12.11/8.12.11) with SMTP id k0AG60vS020160 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2006 11:06:00 -0500 6, 21 -- Received: from post.aecom.yu.edu ([129.98.1.100]) 6, 21 -- by mailvx.aecom.yu.edu (SAVSMTP 3.1.1.32) with SMTP id M2006011011062608439 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2006 11:06:26 -0500 6, 21 -- Received: from [129.98.90.160] (worm.aecom.yu.edu [129.98.90.160]) 6, 21 -- by post.aecom.yu.edu (Postfix) with ESMTP id 340A12FC0 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2006 11:06:00 -0500 (EST) 6, 21 -- Mime-Version: 1.0 6, 21 -- X-Sender: hall-at-mailserver.aecom.yu.edu 6, 21 -- Message-Id: {a05100316bfe98b41a356-at-[129.98.90.160]} 6, 21 -- Date: Tue, 10 Jan 2006 11:05:56 -0500 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- From: David Hall {hall-at-aecom.yu.edu} 6, 21 -- Subject: glue to make ribbon's stick together 6, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Matt, I don't have experience to work with vanadium oxide, but some superconductive materials which were not working well with water. If you use water only for your grinding procedure for your case, now you can try dry-grinding, at least for the final stage. or you can try leaving thicker for the final ion mill (like 45 micron) if it is not hard to be ion-milled under Ar+ ion beam, but this may cause some beam damage or contamination if there is not cold stage and the milling time is too long. again to try higher rate for the early milling stage and to reduce it at the final may help with this. or you could try methanol or ethanol for your grinding if the "wet" procedure is necessary. also you can try FIB. it is dry, but may consider its beam damage if your material is sensitive to this. Hope it can help
Sincerely Long Li _________________________________________________________ Materials Science and Engineering Department University of Pittsburgh 3700 O'Hara St., 848 Benedum Hall Pittsburgh, PA 15261
----- Original Message ----- X-from: {mjo10-at-psu.edu} To: {Longli_tem-at-hotmail.com} Sent: Monday, January 09, 2006 7:39 PM
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Email: mkamrath-at-nalco.com Name: Mike Kamrath
Organization: Nalco Co., Naperville IL
Title-Subject: [Filtered] Colloidal Silica Particle Size Distribution by TEM
Question: I was looking for help with a problem we have related to particle size distributions (PSD) with colloidal silicas as measured by TEM. We normally dilute a colloidal silica solution in an aqueous solution containing a few drops of nonionic surfactant to help disperse the particles (5-100 nm). After placing a drop on a copper grid (Formvar) we dry it at 100 oC for 10 minutes and place in the TEM for PSD. We are trying to automate the process with counting software but the software has problems identifying individual particles when they are touching one another. I have two questions related to this: 1) Does anyone know of software capable of easily separating touching particles and counting individually? We are currently using Image-Pro Plus and I am aware of free software from NIH (Scion) which seems to have similar problems. 2) Does anybody have suggestions for a better solvent system or surfactant that might separate the particles better? Or is the drying process forcing the particles together and would a vacuum drying or other method work better?
A couple of months ago, I repumped in situ my chronically leaky PGT Be-window EDS detector, and ran 50 deg C air into the dewar while re-evacuating using the chamber vacuum via a hose from the sample introduction port of the JEOL 840 on which the detector is mounted.
The re-evacuation worked well, in that the LN2 consumption is now back to normal, but after recooling and switching on the bias again, there was no appreciable low-energy response -- no visible Cu L peak at all!
I realised that grease from the O-ring sliding seal through which the tube of the detector enters the chamber had melted and run down onto the Be window. Cleaning of the window with Freon restored the Cu L intensity, and all was well, until it became obvious that the window was re-oiling again at a much faster rate than ever before.
Because we do quantitative mineral analysis, I keep a good handle on the window condition, evidenced by both Cu L and Na K responses. I have been using this detector for about five years and not found it neccesary to clean the window before, but now the Na response halves in a few weeks. Recleaning with Freon restores the response again.
In an effort to fix this, I have:-
- changed the rotary pump oil - installed an alumina-pellet foreline trap - changed the DP oil (Santovac, but the old charge was still light-colored)
but still the darned window is oiling up.
I'm running out of ideas. It seems that either the chamber atmosphere has become markedly oilier than before, or the detector window is now running colder than before.
My money is on the latter, as it seems too much of a coincidence for some change in the back-streaming performance of the 840 to have occurred at the same time as the repumping of the detector, but I can't see what might have happened.
Anyone got any suggestions?
Happy New Year
rtch
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 16, 26 -- From r.sims-at-auckland.ac.nz Tue Jan 10 19:00:56 2006 16, 26 -- Received: from chico.itss.auckland.ac.nz (chico.itss.auckland.ac.nz [130.216.190.12]) 16, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0B10td7001103 16, 26 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2006 19:00:56 -0600 16, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 16, 26 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id F369034958 16, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 14:00:53 +1300 (NZDT) 16, 26 -- Received: from chico.itss.auckland.ac.nz ([127.0.0.1]) 16, 26 -- by localhost (smtpb.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 16, 26 -- with ESMTP id 31308-14 for {microscopy-at-microscopy.com} ; 16, 26 -- Wed, 11 Jan 2006 14:00:46 +1300 (NZDT) 16, 26 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) 16, 26 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id 29EF235637 16, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 13:59:05 +1300 (NZDT) 16, 26 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 16, 26 -- To: microscopy-at-microscopy.com 16, 26 -- Date: Wed, 11 Jan 2006 13:59:04 +1300 16, 26 -- MIME-Version: 1.0 16, 26 -- Subject: Oil on detector window 16, 26 -- Message-ID: {43C50F28.11970.13D9DFF-at-localhost} 16, 26 -- Priority: normal 16, 26 -- X-mailer: Pegasus Mail for Windows (4.21c) 16, 26 -- Content-type: text/plain; charset=US-ASCII 16, 26 -- Content-transfer-encoding: 7BIT 16, 26 -- Content-description: Mail message body 16, 26 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
First, may I ask what you have done to ensure that your support films are hydrophilic? Carbon coated polyvinylformal supports become increasingly hydrophobic with age. We get good results by treating with spectro-grade acetone for 30 min prior to use. We have also used a short plasma-ash in air to improve wettability. Such treatments improves dispersion and reduces drying artifacts.
We use the analySIS Five software from Soft Imaging System for image analysis. This program has a useful separator based upon a watershed algorithm. I have also written a custom watershed module for some applications. We also use a guard frame to eliminate bias from the higher probability that larger particles touch the image boundaries compared to smaller particles. We prefer this approach to the Miles-Lantejoul algorithm. Using an extension to the analySIS Automater we can queue up images from several samples from any of our microscopes and let them run unattended.
We have compared results obtained by simply deleting agglomerates with the results obtained by separation. Even if we use a separator, we typically measure the maximum intrusion distance [this is a custom module we wrote] for each particle and flag those which are not convex as agglomerates. We always keep track of the area fraction of agglomerates and reject analyses where this is too high. Usually we can find preparation conditions to keep the area fraction of agglomerates below 10%.
One of the strengths of the analySIS program is that the macro language is almost a full subset of C and if one needs a computationally-intensive function (like the maximum intrusion distance) it is easy to import a C function from a custom DLL compiled with a good optimizing compiler, like Visual C++.
Disclaimer: I have no financial interest in Soft Imaging System, writing only as a satisfied user.
John R. Minter Eastman Kodak Co. Research Laboratories Foundation Science and Technology Center john.r.minter-at-kodak.com (work) jrminter-at-rochester.rr.com (home)
-----Original Message----- X-from: mkamrath-at-nalco.com [mailto:mkamrath-at-nalco.com] Sent: Tuesday, January 10, 2006 7:28 PM To: jrminter-at-rochester.rr.com
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please copy both mkamrath-at-nalco.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mkamrath-at-nalco.com Name: Mike Kamrath
Organization: Nalco Co., Naperville IL
Title-Subject: [Filtered] Colloidal Silica Particle Size Distribution by TEM
Question: I was looking for help with a problem we have related to particle size distributions (PSD) with colloidal silicas as measured by TEM. We normally dilute a colloidal silica solution in an aqueous solution containing a few drops of nonionic surfactant to help disperse the particles (5-100 nm). After placing a drop on a copper grid (Formvar) we dry it at 100 oC for 10 minutes and place in the TEM for PSD. We are trying to automate the process with counting software but the software has problems identifying individual particles when they are touching one another. I have two questions related to this: 1) Does anyone know of software capable of easily separating touching particles and counting individually? We are currently using Image-Pro Plus and I am aware of free software from NIH (Scion) which seems to have similar problems. 2) Does anybody have suggestions for a better solvent system or surfactant that might separate the particles better? Or is the drying p! rocess forcing the particles together and would a vacuum drying or other method work better?
I am looking for a simple probe-type sonicator that will be used to make holey films.
We have used an old one in the past that is no longer available. It is used to aerate a formvar/glycerol solution sufficiently to make very small bubbles. When a slide is dipped into the solution, the resulting film has holes of a size dependent on the length of time used for the sonication.
Any recommendations welcomed but hopefully I can find one on the less expensive side.
Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 6, 21 -- From dsherman-at-purdue.edu Wed Jan 11 08:11:13 2006 6, 21 -- Received: from exchange.purdue.edu (1061exfe03.adpc.purdue.edu [128.210.63.225]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0BEBDF0028321 6, 21 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 08:11:13 -0600 6, 21 -- Received: from EXCH02.purdue.lcl ([128.210.63.235]) by exchange.purdue.edu with Microsoft SMTPSVC(6.0.3790.211); 6, 21 -- Wed, 11 Jan 2006 09:10:23 -0500 6, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH02.purdue.lcl ([128.210.63.237]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.239]) with Microsoft Exchange Server HTTP-DAV ; 6, 21 -- Wed, 11 Jan 2006 14:10:15 +0000 6, 21 -- User-Agent: Microsoft-Entourage/11.2.1.051004 6, 21 -- Date: Wed, 11 Jan 2006 09:11:05 -0500 6, 21 -- Subject: Sonicator 6, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 6, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 21 -- Message-ID: {BFEA7C29.C52D%dsherman-at-purdue.edu} 6, 21 -- Thread-Topic: Sonicator 6, 21 -- Thread-Index: AcYWuNwnGsctLIKsEdqMVgARJN08Mg== 6, 21 -- Mime-version: 1.0 6, 21 -- Content-type: text/plain; 6, 21 -- charset="US-ASCII" 6, 21 -- Content-transfer-encoding: 7bit 6, 21 -- X-OriginalArrivalTime: 11 Jan 2006 14:10:23.0149 (UTC) FILETIME=[C3356DD0:01C616B8] ==============================End of - Headers==============================
sue- For what it's worth, and to get in synch with anyone out there trying tests such as i described, last week I repeated my 40 year old MnO4 tests in water on all the liquid resin components, adding 1-2 small drops to 2.5 ml dilute transparent purple MnO4 sol'n (2.5 ml water, 2 drops 2% KMnO4). I found that NMA instantly turns the sol'n to clear brown (guess I'd misremembered that it made a precipitate before), and DMP-30 does so almost as fast. All the other components, including Araldite 506, do the same thing over periods of 5-200 minutes, Araldite 506 clearly slower than Epon 812. Some make a precipitate, some don't. By next day, nothing retains any purple, all test sol'ns are brown. So the lesson is, MnO4 can act eventually destructively on any sections or their components, given time enough. -mike-
} hi mike...thanks for all your help!!! } sue
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
I am looking into acquiring a new field emission SEM which will be used mainly for failure analysis of metals, but also plastics, paper, an occasional drop of grease, and anything else that wanders through the lab (but rarely biological samples). The field emission SEMs that I am considering are the JEOL JSM-7000F, the ZEISS Supra 40 (VP), the Hitachi S-4300SE/N, and the FEI Quanta 600 FEG. For microanalysis systems, I am considering the newest versions from Thermoelectron, Oxford and EDAX. I welcome any comments that you can offer as to the function (or malfunction) of the above instruments and anaylsis systems, as well as possible electro-magnetic interference issues, user interface issues, interface problems between the SEM and microanalysis systems, and the reliability/response time of the service representatives from the above companies.
Thank you very much for your time!
Sincerely,
Robin Moresi General Dynamics - Advanced Information Systems 100 Plastics Ave. Pittsfield, MA 01201
Tel. (413) 662-2889
==============================Original Headers============================== 8, 26 -- From Robin.Moresi-at-gd-ais.com Wed Jan 11 11:21:00 2006 8, 26 -- Received: from mnblin01.mnb.gd-ais.com (mnblin01.mnb.gd-ais.com [206.11.149.28]) 8, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0BHKxPT012197 8, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 11:20:59 -0600 8, 26 -- Received: from mnbm01-fes01.ad.gd-ais.com (mnbm01-fes01.mnb.gd-ais.com [160.207.224.15]) 8, 26 -- by mnblin01.mnb.gd-ais.com (8.12.11/8.12.11) with SMTP id k0BHMC0b006944 8, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 11:22:12 -0600 8, 26 -- Received: from MAPF01-MAIL01.ad.gd-ais.com ([166.16.220.104]) by mnbm01-fes01.ad.gd-ais.com with Microsoft SMTPSVC(6.0.3790.1830); 8, 26 -- Wed, 11 Jan 2006 11:21:06 -0600 8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 8, 26 -- Content-class: urn:content-classes:message 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-Type: text/plain; 8, 26 -- charset="us-ascii" 8, 26 -- Subject: Field Emission SEM question 8, 26 -- Date: Wed, 11 Jan 2006 12:20:47 -0500 8, 26 -- Message-ID: {5DC7DC21AC968A46AEF8265C6D623C15957A70-at-MAPF01-MAIL01.ad.gd-ais.com} 8, 26 -- X-MS-Has-Attach: 8, 26 -- X-MS-TNEF-Correlator: 8, 26 -- Thread-Topic: Field Emission SEM question 8, 26 -- Thread-Index: AcYWzfPqVEoK4BtUSPGMgcimUwgWZgAADOoAAAEzsbA= 8, 26 -- From: "Moresi, Robin T." {Robin.Moresi-at-gd-ais.com} 8, 26 -- To: {microscopy-at-microscopy.com} 8, 26 -- X-OriginalArrivalTime: 11 Jan 2006 17:21:06.0964 (UTC) FILETIME=[68410540:01C616D3] 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0BHKxPT012197 ==============================End of - Headers==============================
I am looking for a manual for a SORVALL MT2-B ultra microtome. I will be happy to pay for copying and shipping costs, but would greatly appreciate if someone would be willing to copy their manual for me.
Thanks, Elke
Elke Buschbeck Biological Sciences University of Cincinnati Cincinnati, OH elke.buschbeck-at-uc.edu
==============================Original Headers============================== 6, 17 -- From elke.buschbeck-at-uc.edu Wed Jan 11 13:22:43 2006 6, 17 -- Received: from ms-smtp-02-eri0.ohiordc.rr.com (ms-smtp-02-smtplb.ohiordc.rr.com [65.24.5.136]) 6, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0BJMeEv026815 6, 17 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 13:22:42 -0600 6, 17 -- Received: from Mainbrain.uc.edu (cpe-65-27-235-87.cinci.res.rr.com [65.27.235.87]) 6, 17 -- by ms-smtp-02-eri0.ohiordc.rr.com (8.12.10/8.12.7) with ESMTP id k0BJMZg6002796 6, 17 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 14:22:37 -0500 (EST) 6, 17 -- Message-Id: {6.1.2.0.2.20060111141640.08deb160-at-ucmail8.uc.edu} 6, 17 -- X-Sender: buschbek-at-ucmail8.uc.edu 6, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 6.1.2.0 6, 17 -- Date: Wed, 11 Jan 2006 14:22:35 -0500 6, 17 -- To: Microscopy-at-microscopy.com 6, 17 -- From: Elke Buschbeck {elke.buschbeck-at-uc.edu} 6, 17 -- Subject: Need manual for Sorvall MT2-B ultra microtome 6, 17 -- Mime-Version: 1.0 6, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 17 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
What has happened to you has happened to many before you!
Once you reach the point of contaminating your window with RP fluid it will have contaminated the complete vacuum system. Two solutions. EITHER take everything apart and clean it, yes ALL the vacuum lines and column liners. OR use a hair drier to bake the pumping lines to drive off the contamination as best as possible, as well as cleaning the parts you are easily able to reach.
Many times in my service technician career I have been forced to take this route. It puts you out of action for a day or so whilst the system recovers but in the main it seems to be a cure. BUT if you do not tackle the fore line trap idea it will happen all over again - our chemical test said it was RP fluid by the way.
Good luck
Steve Chapman Senior Consultant Protrain For electron microscopy consultancy and training world wide Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7802 966067 Web www.emcourses.com
----- Original Message ----- X-from: {r.sims-at-auckland.ac.nz} To: {protrain-at-emcourses.com} Sent: Wednesday, January 11, 2006 1:02 AM
Will the person that wanted the MT2-B manual please contact me. I found the one I was looking for after I deleted your post.
Mannie Steglich msteglic-at-mdanderson.org
==============================Original Headers============================== 2, 15 -- From msteglic-at-mdanderson.org Wed Jan 11 15:43:11 2006 2, 15 -- Received: from UTM-MAIL04A.mdacc.tmc.edu (utmlnmail04nt.mdacc.tmc.edu [143.111.84.152]) 2, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0BLhB5n014618 2, 15 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 11 Jan 2006 15:43:11 -0600 2, 15 -- To: Microscopy-at-MSA.Microscopy.Com 2, 15 -- Subject: MT2-B Manual 2, 15 -- MIME-Version: 1.0 2, 15 -- X-Mailer: Lotus Notes Release 5.0.11 July 24, 2002 2, 15 -- Message-ID: {OF72D353B1.75A3682D-ON862570F3.007713B0-862570F3.00774DE6-at-mdacc.tmc.edu} 2, 15 -- From: msteglic-at-mdanderson.org 2, 15 -- Date: Wed, 11 Jan 2006 15:42:54 -0600 2, 15 -- X-MIMETrack: Serialize by Router on UTM-MAIL04A/HOU/UTMDACC(Release 5.0.11 |July 24, 2002) at 2, 15 -- 01/11/2006 03:42:58 PM, 2, 15 -- Serialize complete at 01/11/2006 03:42:58 PM 2, 15 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Many thanks for the replies and suggestions so far, however, please note that this is a new condition.
For the past five years the oiling has not been a problem, and it has started to occur only since the repumping operation described below in tedious detail.
There have been no changes to the system apart from the three steps taken, one at a time, in an attempt to fix the problem (changing the RP oil; installing the foreline trap; and changing the DP oil), and they have made no difference.
I think it would be too much of a coincidence for the cause of the oiling to be unrelated to the repumping operation, so I think that complex cures such as installing LN2 traps, plasma cleaning, complete stripping and cleaning, etc, do not address the question, which is "what has changed to accelerate the detector window oiling so greatly, and how can I get back to the previous state?"
I had thought that perhaps a drop of melted grease might have dripped into the DP, and be decomposing in the Santovac, which propelled me to change the Santovac, but I now realise that this is very unlikely to be the case, as the DP is not directly beneath the sample chamber, and even if it were, such a drop of molten grease would not make it through the water-cooled baffle above the DP. Plus, of course, the fact that very thoroughly cleaning both of the DPs and both of their water-cooled baffles has made no difference at all to the problem.
Incidentally, the Santovac, after five years' continuous operation, was only a pale straw color, boy, that stuff certainly is stable, isn't it?
I feel fairly sure that there is a simple solution to my problem, waiting out there to be noticed, so please keep the suggestions coming.
cheers
rtch
On 10 Jan 2006 at 19:02, r.sims-at-auckland.ac.nz wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Here's a problem for the New Year: } } A couple of months ago, I repumped in situ my chronically leaky PGT Be-window EDS } detector, and ran 50 deg C air into the dewar while re-evacuating using the chamber } vacuum via a hose from the sample introduction port of the JEOL 840 on which the } detector is mounted. } } The re-evacuation worked well, in that the LN2 consumption is now back to normal, but } after recooling and switching on the bias again, there was no appreciable low-energy } response -- no visible Cu L peak at all! } } I realised that grease from the O-ring sliding seal through which the tube of the detector } enters the chamber had melted and run down onto the Be window. Cleaning of the } window with Freon restored the Cu L intensity, and all was well, until it became obvious } that the window was re-oiling again at a much faster rate than ever before. } } Because we do quantitative mineral analysis, I keep a good handle on the window } condition, evidenced by both Cu L and Na K responses. I have been using this detector } for about five years and not found it neccesary to clean the window before, but now the } Na response halves in a few weeks. Recleaning with Freon restores the response } again. } } In an effort to fix this, I have:- } } - changed the rotary pump oil } - installed an alumina-pellet foreline trap } - changed the DP oil (Santovac, but the old charge was still light-colored) } } but still the darned window is oiling up. } } I'm running out of ideas. It seems that either the chamber atmosphere has become } markedly oilier than before, or the detector window is now running colder than before. } } My money is on the latter, as it seems too much of a coincidence for some change in } the back-streaming performance of the 840 to have occurred at the same time as the } repumping of the detector, but I can't see what might have happened. } } Anyone got any suggestions? } } Happy New Year } } rtch } } } -- } Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 } Microanalyst Fax : 64 9 3737435 } Department of Geology email : r.sims-at-auckland.ac.nz } The University of Auckland } Private Bag 92019 } Auckland } New Zealand } } } ==============================Original Headers============================== } 16, 26 -- From r.sims-at-auckland.ac.nz Tue Jan 10 19:00:56 2006 } 16, 26 -- Received: from chico.itss.auckland.ac.nz (chico.itss.auckland.ac.nz [130.216.190.12]) } 16, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0B10td7001103 } 16, 26 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2006 19:00:56 -0600 } 16, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 16, 26 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id F369034958 } 16, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 14:00:53 +1300 (NZDT) } 16, 26 -- Received: from chico.itss.auckland.ac.nz ([127.0.0.1]) } 16, 26 -- by localhost (smtpb.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) } 16, 26 -- with ESMTP id 31308-14 for {microscopy-at-microscopy.com} ; } 16, 26 -- Wed, 11 Jan 2006 14:00:46 +1300 (NZDT) } 16, 26 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) } 16, 26 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id 29EF235637 } 16, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 13:59:05 +1300 (NZDT) } 16, 26 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} } 16, 26 -- To: microscopy-at-microscopy.com } 16, 26 -- Date: Wed, 11 Jan 2006 13:59:04 +1300 } 16, 26 -- MIME-Version: 1.0 } 16, 26 -- Subject: Oil on detector window } 16, 26 -- Message-ID: {43C50F28.11970.13D9DFF-at-localhost} } 16, 26 -- Priority: normal } 16, 26 -- X-mailer: Pegasus Mail for Windows (4.21c) } 16, 26 -- Content-type: text/plain; charset=US-ASCII } 16, 26 -- Content-transfer-encoding: 7BIT } 16, 26 -- Content-description: Mail message body } 16, 26 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz } ==============================End of - Headers==============================
==============================Original Headers============================== 15, 27 -- From r.sims-at-auckland.ac.nz Wed Jan 11 17:09:46 2006 15, 27 -- Received: from groucho.itss.auckland.ac.nz (groucho.itss.auckland.ac.nz [130.216.190.11]) 15, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0BN9jBt024828 15, 27 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 17:09:46 -0600 15, 27 -- Received: from localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) 15, 27 -- by groucho.itss.auckland.ac.nz (Postfix) with ESMTP id 3333B352E7 15, 27 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 12:09:44 +1300 (NZDT) 15, 27 -- Received: from groucho.itss.auckland.ac.nz ([127.0.0.1]) 15, 27 -- by localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 15, 27 -- with ESMTP id 13091-10 for {microscopy-at-microscopy.com} ; 15, 27 -- Thu, 12 Jan 2006 12:09:44 +1300 (NZDT) 15, 27 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) 15, 27 -- by groucho.itss.auckland.ac.nz (Postfix) with ESMTP id 0E039343ED 15, 27 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 12:09:44 +1300 (NZDT) 15, 27 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 15, 27 -- To: microscopy-at-microscopy.com 15, 27 -- Date: Thu, 12 Jan 2006 12:12:02 +1300 15, 27 -- MIME-Version: 1.0 15, 27 -- Subject: More about oil on detector window 15, 27 -- Message-ID: {43C64792.29976.BC74A1-at-localhost} 15, 27 -- Priority: normal 15, 27 -- In-reply-to: {200601110102.k0B12645002985-at-ns.microscopy.com} 15, 27 -- X-mailer: Pegasus Mail for Windows (4.21c) 15, 27 -- Content-type: text/plain; charset=US-ASCII 15, 27 -- Content-transfer-encoding: 7BIT 15, 27 -- Content-description: Mail message body 15, 27 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
Dear Ritch, Does the snout of the EDS detector feel cool to the touch, when you vent the chamber? (Use the back of your hand to test it.) I had one detector that oiled very badly and it felt cool to the touch, so it acted as an oil trap for the chamber. I sent that detector to be completely rebuilt as a light element, high resolution detector and that problem has vanished, so it was a condition of the detector, not the SEM, since the SEM was not touched. It may be that the insulation between the liquid nitrogen or the cooled components and the snout of the detector has changed with your warm-up and re-pump. I am wondering if the copper braid that conducts the cool from the dewar to the nose of the detector has moved or is touching something. None of this helps you in any way. You can clean the Be-window detector with a trickle of pure ethanol or iso-propanol in the SEM, if you can get at it. ----- Original Message ----- X-from: {r.sims-at-auckland.ac.nz} To: {mager-at-interchange.ubc.ca} Sent: Wednesday, January 11, 2006 3:13 PM
Ritch, This is a long shot and you probably would have noticed the symptoms, but here goes:
I had one 840 that kept being "burped" first thing in the morning, but not after that. The key to finding the problem was that the LEDs on the vacuum control all cycled correctly, but it took me a while (since it only happened once a day) to notice that the actual valve operation for V2 (at the base of the second DP) was delayed just long enough to burp the DPs. Turns out the solenoid valve that operated V2 had Apiezon in it (a lot) and after sitting overnight would stick for about 2 seconds, only milliseconds longer than the delay for V5 to rough the load lock. This problem had been intermittent for years before I took on the service contract, but things work fine, now.
Perhaps you have a similar timing problem. Of course, the most obvious thing was that the DPs dumped and you don't seem to have had anything that severe happening.
Another thought: when you cleaned the DPs, did you also remove and clean the ballast tank located between them? Perhaps it has become too contaminated and is now backstreaming since there is only one DP between it and the chamber.
Do you have any additional gauging to monitor the actual pressure in the chamber? There might be some clues there, also. I'm thinking along the lines of a slightly leaky V4.
One last thought: Is the water flowing in the correct direction? An accidental reversal puts warm water running through the water baffles and virtually always results in oil on the EDS detector.
Best of luck, Ken Converse
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: r.sims-at-auckland.ac.nz [mailto:r.sims-at-auckland.ac.nz] Sent: Wednesday, January 11, 2006 6:12 PM To: kenconverse-at-qualityimages.biz
Many thanks for the replies and suggestions so far, however, please note that this is a new condition.
For the past five years the oiling has not been a problem, and it has started to occur only since the repumping operation described below in tedious detail.
There have been no changes to the system apart from the three steps taken, one at a time, in an attempt to fix the problem (changing the RP oil; installing the foreline trap; and changing the DP oil), and they have made no difference.
I think it would be too much of a coincidence for the cause of the oiling to be unrelated to the repumping operation, so I think that complex cures such as installing LN2 traps, plasma cleaning, complete stripping and cleaning, etc, do not address the question, which is "what has changed to accelerate the detector window oiling so greatly, and how can I get back to the previous state?"
I had thought that perhaps a drop of melted grease might have dripped into the DP, and be decomposing in the Santovac, which propelled me to change the Santovac, but I now realise that this is very unlikely to be the case, as the DP is not directly beneath the sample chamber, and even if it were, such a drop of molten grease would not make it through the water-cooled baffle above the DP. Plus, of course, the fact that very thoroughly cleaning both of the DPs and both of their water-cooled baffles has made no difference at all to the problem.
Incidentally, the Santovac, after five years' continuous operation, was only a pale straw color, boy, that stuff certainly is stable, isn't it?
I feel fairly sure that there is a simple solution to my problem, waiting out there to be noticed, so please keep the suggestions coming.
cheers
rtch
On 10 Jan 2006 at 19:02, r.sims-at-auckland.ac.nz wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Here's a problem for the New Year: } } A couple of months ago, I repumped in situ my chronically leaky PGT Be-window EDS } detector, and ran 50 deg C air into the dewar while re-evacuating using the chamber } vacuum via a hose from the sample introduction port of the JEOL 840 on which the } detector is mounted. } } The re-evacuation worked well, in that the LN2 consumption is now back to normal, but } after recooling and switching on the bias again, there was no appreciable low-energy } response -- no visible Cu L peak at all! } } I realised that grease from the O-ring sliding seal through which the tube of the detector } enters the chamber had melted and run down onto the Be window. Cleaning of the } window with Freon restored the Cu L intensity, and all was well, until it became obvious } that the window was re-oiling again at a much faster rate than ever before. } } Because we do quantitative mineral analysis, I keep a good handle on the window } condition, evidenced by both Cu L and Na K responses. I have been using this detector } for about five years and not found it neccesary to clean the window before, but now the } Na response halves in a few weeks. Recleaning with Freon restores the response } again. } } In an effort to fix this, I have:- } } - changed the rotary pump oil } - installed an alumina-pellet foreline trap } - changed the DP oil (Santovac, but the old charge was still light-colored) } } but still the darned window is oiling up. } } I'm running out of ideas. It seems that either the chamber atmosphere has become } markedly oilier than before, or the detector window is now running colder than before. } } My money is on the latter, as it seems too much of a coincidence for some change in } the back-streaming performance of the 840 to have occurred at the same time as the } repumping of the detector, but I can't see what might have happened. } } Anyone got any suggestions? } } Happy New Year } } rtch } } } -- } Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 } Microanalyst Fax : 64 9 3737435 } Department of Geology email : r.sims-at-auckland.ac.nz } The University of Auckland } Private Bag 92019 } Auckland } New Zealand } } } ==============================Original Headers============================== } 16, 26 -- From r.sims-at-auckland.ac.nz Tue Jan 10 19:00:56 2006 } 16, 26 -- Received: from chico.itss.auckland.ac.nz (chico.itss.auckland.ac.nz [130.216.190.12]) } 16, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0B10td7001103 } 16, 26 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2006 19:00:56 -0600 } 16, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 16, 26 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id F369034958 } 16, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 14:00:53 +1300 (NZDT) } 16, 26 -- Received: from chico.itss.auckland.ac.nz ([127.0.0.1]) } 16, 26 -- by localhost (smtpb.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) } 16, 26 -- with ESMTP id 31308-14 for {microscopy-at-microscopy.com} ; } 16, 26 -- Wed, 11 Jan 2006 14:00:46 +1300 (NZDT) } 16, 26 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) } 16, 26 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id 29EF235637 } 16, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 13:59:05 +1300 (NZDT) } 16, 26 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} } 16, 26 -- To: microscopy-at-microscopy.com } 16, 26 -- Date: Wed, 11 Jan 2006 13:59:04 +1300 } 16, 26 -- MIME-Version: 1.0 } 16, 26 -- Subject: Oil on detector window } 16, 26 -- Message-ID: {43C50F28.11970.13D9DFF-at-localhost} } 16, 26 -- Priority: normal } 16, 26 -- X-mailer: Pegasus Mail for Windows (4.21c) } 16, 26 -- Content-type: text/plain; charset=US-ASCII } 16, 26 -- Content-transfer-encoding: 7BIT } 16, 26 -- Content-description: Mail message body } 16, 26 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz } ==============================End of - Headers==============================
==============================Original Headers============================== 15, 27 -- From r.sims-at-auckland.ac.nz Wed Jan 11 17:09:46 2006 15, 27 -- Received: from groucho.itss.auckland.ac.nz (groucho.itss.auckland.ac.nz [130.216.190.11]) 15, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0BN9jBt024828 15, 27 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 17:09:46 -0600 15, 27 -- Received: from localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) 15, 27 -- by groucho.itss.auckland.ac.nz (Postfix) with ESMTP id 3333B352E7 15, 27 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 12:09:44 +1300 (NZDT) 15, 27 -- Received: from groucho.itss.auckland.ac.nz ([127.0.0.1]) 15, 27 -- by localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 15, 27 -- with ESMTP id 13091-10 for {microscopy-at-microscopy.com} ; 15, 27 -- Thu, 12 Jan 2006 12:09:44 +1300 (NZDT) 15, 27 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) 15, 27 -- by groucho.itss.auckland.ac.nz (Postfix) with ESMTP id 0E039343ED 15, 27 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 12:09:44 +1300 (NZDT) 15, 27 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 15, 27 -- To: microscopy-at-microscopy.com 15, 27 -- Date: Thu, 12 Jan 2006 12:12:02 +1300 15, 27 -- MIME-Version: 1.0 15, 27 -- Subject: More about oil on detector window 15, 27 -- Message-ID: {43C64792.29976.BC74A1-at-localhost} 15, 27 -- Priority: normal 15, 27 -- In-reply-to: {200601110102.k0B12645002985-at-ns.microscopy.com} 15, 27 -- X-mailer: Pegasus Mail for Windows (4.21c) 15, 27 -- Content-type: text/plain; charset=US-ASCII 15, 27 -- Content-transfer-encoding: 7BIT 15, 27 -- Content-description: Mail message body 15, 27 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
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==============================Original Headers============================== 36, 23 -- From kenconverse-at-qualityimages.biz Thu Jan 12 09:30:50 2006 36, 23 -- Received: from qualityimages.biz (dpmail16.doteasy.com [65.61.209.16]) 36, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0CFUnmn022474 36, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 12 Jan 2006 09:30:49 -0600 36, 23 -- Received: from Ken [68.64.242.101] by qualityimages.biz with ESMTP 36, 23 -- (SMTPD32-8.05) id A61691000C4; Thu, 12 Jan 2006 07:30:30 -0800 36, 23 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 36, 23 -- To: {r.sims-at-auckland.ac.nz} , "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 36, 23 -- Subject: RE: [Microscopy] More about oil on detector window 36, 23 -- Date: Thu, 12 Jan 2006 10:30:26 -0500 36, 23 -- Message-ID: {003701c6178d$2273cd80$6501a8c0-at-Ken} 36, 23 -- MIME-Version: 1.0 36, 23 -- Content-Type: text/plain; 36, 23 -- charset="us-ascii" 36, 23 -- X-Priority: 3 (Normal) 36, 23 -- X-MSMail-Priority: Normal 36, 23 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 36, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 36, 23 -- In-Reply-To: {200601112312.k0BNCQVD030081-at-ns.microscopy.com} 36, 23 -- Importance: Normal 36, 23 -- X-IMSTrailer: __IMail_7__ 36, 23 -- Content-Transfer-Encoding: 8bit 36, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0CFUnmn022474 ==============================End of - Headers==============================
Transmission Electron Microscopy (http://www.collegeofmicroscopy.com/courses/17.asp)
Both are hands-on, introductory level courses taught using the latest equipment available. Please follow the links provided for further details and registration information.
Elaine Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 11, 27 -- From eschumacher-at-mccrone.com Thu Jan 12 14:13:16 2006 11, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122]) 11, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0CKDGdt003847 11, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 12 Jan 2006 14:13:16 -0600 11, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 11, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 4FC601A800B 11, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 12 Jan 2006 14:13:17 -0600 (CST) 11, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 11, 27 -- by pgp.mccrone.com (PGP Universal service); 11, 27 -- Thu, 12 Jan 2006 14:13:17 -0600 11, 27 -- X-PGP-Universal: processed 11, 27 -- Content-class: urn:content-classes:message 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- charset="US-ASCII" 11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 11, 27 -- Subject: Short Course Announcement: Scientific Imaging, Transmission Electron Microscopy 11, 27 -- Date: Thu, 12 Jan 2006 14:13:06 -0600 11, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A74C30F7-at-MCCRONEMSG.tmg.mccrone.com} 11, 27 -- X-MS-Has-Attach: 11, 27 -- X-MS-TNEF-Correlator: 11, 27 -- Thread-Topic: Short Course Announcement: Scientific Imaging, Transmission Electron Microscopy 11, 27 -- Thread-Index: AcYXtJk/0FDZxb4QTf2pOACZsYbNJQ== 11, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 11, 27 -- To: {Microscopy-at-MSA.Microscopy.Com} 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0CKDGdt003847 ==============================End of - Headers==============================
Does anyone know of a reasonable alternative to the venerable Zero Stat static taming guns? We use them often but there's just got to be a better way. I used to smuggle squirt guns onto the school bus that were made better (the good old days), and they didn't cost over $100 for about $1 worth of components.
Thanks and Happy New Year to all.
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 9, 23 -- From TindallR-at-missouri.edu Thu Jan 12 16:59:22 2006 9, 23 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49]) 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0CMxMkH015190 9, 23 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 16:59:22 -0600 9, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 9, 23 -- Thu, 12 Jan 2006 16:59:22 -0600 9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 23 -- Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="us-ascii" 9, 23 -- Subject: Static guns? 9, 23 -- Date: Thu, 12 Jan 2006 16:59:21 -0600 9, 23 -- Message-ID: {BA876152E8653240BE8572E897083EE701849CB7-at-UM-EMAIL09.um.umsystem.edu} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: Static guns? 9, 23 -- Thread-Index: AcYXy9ND13Q0Mk2eRQC7DwCisMtGXA== 9, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 9, 23 -- To: {microscopy-at-microscopy.com} 9, 23 -- X-OriginalArrivalTime: 12 Jan 2006 22:59:22.0766 (UTC) FILETIME=[D3E862E0:01C617CB] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0CMxMkH015190 ==============================End of - Headers==============================
Job Opening #B005608; Lab Technician/Engineer at IBM
There is an opening at the Lab Technician/Engineer level within the Materials Characterization and Analysis Group in the Research Division of IBM at the Almaden Research Center in San Jose, California. The job is a long term (three years) supplemental position with benefits. A B.S. in material science or equivalent fields, or successful completion of a two year college program plus experience, is required.
This position involves technical support in an advanced electron microscopy laboratory which emphasizes transmission electron microscopy. The technician works as a team member with professional (Ph.D.) microscopists and other scientists in a research laboratory. Duties involve primarily sample preparation methods including the conventional grinding, polishing, and ion milling techniques, focused ion beam (FIB ) method, as well as Microtome, etc. In addition, the candidate must be able to operate and perform routine maintenance on specimen preparation equipment, interact with customers (professional scientists), and prepare reports. The candidate must be able to work independently within set priorities, to keep abreast of new advances, and to interact smoothly within a team.
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Dr. Philip M. Rice IBM Research Division Almaden Research Center 650 Harry Road, K19B/D1 tel: (408) 927-1442 fax: (408) 927-2100 email: pmrice-at-almaden.ibm.com
Dr. Philip M. Rice IBM Research Division Almaden Research Center 650 Harry Road, K19B/D1 San Jose, CA 95120-6099 tel: (408) 927-1442 fax: (408) 927-2100 e-mail: pmrice-at-almaden.ibm.com
==============================Original Headers============================== 10, 26 -- From pmrice-at-almaden.ibm.com Thu Jan 12 17:19:37 2006 10, 26 -- Received: from e6.ny.us.ibm.com (e6.ny.us.ibm.com [32.97.182.146]) 10, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0CNJaZ0024520 10, 26 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 17:19:36 -0600 10, 26 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236]) 10, 26 -- by e6.ny.us.ibm.com (8.12.11/8.12.11) with ESMTP id k0CNJZhi023925 10, 26 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 18:19:35 -0500 10, 26 -- Received: from d01av03.pok.ibm.com (d01av03.pok.ibm.com [9.56.224.217]) 10, 26 -- by d01relay04.pok.ibm.com (8.12.10/NCO/VERS6.8) with ESMTP id k0CNJZBB128016 10, 26 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 18:19:35 -0500 10, 26 -- Received: from d01av03.pok.ibm.com (loopback [127.0.0.1]) 10, 26 -- by d01av03.pok.ibm.com (8.12.11/8.13.3) with ESMTP id k0CNJZ9b030875 10, 26 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 18:19:35 -0500 10, 26 -- Received: from d01ml605.pok.ibm.com (d01ml605.pok.ibm.com [9.56.227.91]) 10, 26 -- by d01av03.pok.ibm.com (8.12.11/8.12.11) with ESMTP id k0CNJZ8m030872 10, 26 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 18:19:35 -0500 10, 26 -- Subject: TEM - Job opening for a TEM Specimen Preparation Lab Technician 10, 26 -- To: microscopy-at-microscopy.com 10, 26 -- X-Mailer: Lotus Notes Release 6.0.2CF1 June 9, 2003 10, 26 -- Message-ID: {OF50DE3E5B.2E1B3545-ON882570F4.007FA844-882570F4.008020F8-at-us.ibm.com} 10, 26 -- From: Philip Rice {pmrice-at-almaden.ibm.com} 10, 26 -- Date: Thu, 12 Jan 2006 15:19:30 -0800 10, 26 -- X-MIMETrack: Serialize by Router on D01ML605/01/M/IBM(Release 7.0HF90 | November 16, 2005) at 10, 26 -- 01/12/2006 18:19:34 10, 26 -- MIME-Version: 1.0 10, 26 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
I am trying to permeabilize some Haemophilus influenzae fixed in 2% paraformaldehyde + 0.2% glutaraldehyde so I can immunostain an intracellular protein. My first attempts to permeabilize with Triton X-100 were surprising unsuccessful. I tried both literature searches and googling this topic but a search of "bacteria" + "permeabilization" pulls up thousands on non-germane citations. I am interested in comments from anybody with expertise in permeabilization, especially in regard to the bacterial target. Thanks, Tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
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Email: aetmicro-at-optonline.net Name: Andrew Thelian
Title-Subject: [Filtered] Philips EM 300 beam issue
Question: Hi All,
I have hit yet another bump in the road...
I was having a brightness issue last week that i thought might be resolved with cleaning the wehnelt assembly...
Now when i energize the filament i get a very small circle on the view screen...it doesnt appear green it looks like a dim reddish color...it also seems to respond to turning the filament knob...
the condenser and deflection knobs dont cause any change...
I have removed all apertures... to see if there was a beam any where ... but no luck...
This Question was submitted to Ask-A-Microscopist by (dustin.adams-at-xerox.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, January 12, 2006 at 17:11:24 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both dustin.adams-at-xerox.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: Hello, I am going to be training soon to become a SEM microscopist for my company, and was curious to see what the salary amounts are for a typical person in my position. I have tried to look for this info online, but wasn't really able to find anything. If someone could provide that information or point me in the right direction; I would greatly appreciate it.
What you see is the light from filament, not e-beam. Whenever electron gun was disturbed, in your case for Wehnelt cleaning, gun tilt must be re-aligned, and perhaps gun shift too. Both these alignments are mechanical on Phil. EM300. Users manual will help if TEM is functioning properly.
Green light or not, you must be able to tell whether HT is present by watching emission current, and emission meter behavior: a) when HT turned on/off; b) emission setting changed while HT is on; c) and HV setting changed while HT is on.
If HT is present, if filament glows, and if emission current behaves normally, do the following: a) select lowest magnification - I believe it is SC (scan) position; b) move all apertures from the beam pass; c) if no beam yet, turn lens currents off one by one and you must see the beam. Be careful not to burn the screen (keep filament under-saturated). Just turning off C1 and C2 will likely bring the beam back, and then work from that point on.
Vitaly Feingold SIA 2773 Heath Line Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com
----- Original Message ----- X-from: {aetmicro-at-optonline.net} To: {vitalylazar-at-att.net} Sent: Thursday, January 12, 2006 9:23 PM
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Email: secr-at-eurmicsoc.org Name: Nick Schryvers
Organization: EMS
Title-Subject: [Filtered] EMS scholarships for IMC16 Msg 2006004
Question: The European Microscopy Society is pleased to be able to offer 4 scholarships of 500 Euro each to young researchers in support of attending the 16th International Microscopy Congress (http://www.imc16.jp/) in Sapporo, Japan, from Sep. 3 till Sep. 8, 2006. Applications should be sent to the EMS secretary (secr-at-eurmicsoc.org) and include a CV and list of publications and copies of the submitted abstract(s) at IMC16. Proof of acceptance of the abstract(s) should follow as soon as these are available.
Deadline of the applications is February 1, 2006 (same date as abstract submission for IMC16)
Only EMS members are eligible and priority will be given to people under the age of 35.
This support was made possible through a generous offer from JEOL Europe (http://www.jeoleuro.com/).
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both secr-at-eurmicsoc.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: secr-at-eurmicsoc.org Name: Nick Schryvers
Organization: EMS
Title-Subject: [Filtered] Royal Microscopical Society ( RMS) Spring School in Electron Microscopy
Royal Microscopical Society ( RMS) Spring School in Electron Microscopy 27-31 March 2006 University of Leeds, UK. Course Organiser: Prof. Rik Brydson
The RMS School consists of a number of parallel courses covering most aspects of electron microscopy in both the physical and life sciences. Students can attend either 4 or 5 days - the 5 day course includes a day of principles of EM lectures for beginners or as a refresher. During the week, students will have the opportunity to choose to attend lectures and workshops from either course, to tailor it to their specific needs.
'All those involved in the organisation of this event are to be congratulated. The complexity of running a school of electron microscopy covering both life and materials sciences has been surpassed with remarkable success with participants, speakers and organisers equally satisfied with the end results.'
Pedro Costa - Participant Spring School in EM 2005
For more information or to book for this course please visit the RMS website: {http://www.rms.org.uk/event_em_school.shtml} http://www.rms.org.uk/event_em_school.shtml
Victoria Lee Conference Manager Royal Microscopical Society 37/38 St Clements, Oxford OX4 1AJ
I always used a sealed Polonium-210 beta source for static problems - Nuclepore Corp used to make a Polonium-210 (sealed in beads) metal strip that had an open ladder arrangement at the top that you simply placed the Nuclepore filters on. It really reduced static. The only downside was the half-life of a few months that meant after a year or so you had to buy another. We also had Zero-Stat gun, but that wasn't used as it simply didn't work. The Nuclepore device seems to have been dropped now, but you can buy a brush that has Polonium-210 trapped within the bristles that should work on similar lines (NRD - StaticMaster Anti-Static Brush - 1" or 3") :
I know that photographers use them to reduce static on film. I've never tried this specific brush though as I have now moved out of this field. Our histologist used something similar on microtome surfaces when sectioning I remember.
Also have a look at ionisers like : http://www.etherton.co.uk/index.html http://www.2spi.com/catalog/photo/antistat.html
Alternatively you can spray a conducting coating onto the plastic to eliminate static (e.g. the Duron anti-static spray sold by Agar Scientific that "even allows uncoated samples to be viewed under 'low resolution' in an SEM").
There's also the anti-static wrist-strap (or just touching the metal earth of a plugged in PC case or wall socket screw head). Plus breathing on the plastic really removes the static (or earthing the material if it conducts), but that suits plastic record player Perspex covers more than scientific specimens. Keeping the room %RH constant and not going too low will also help. Some labs have a full antistatic grounding system with furniture, mats etc.. all 'grounded' with controlled %RH and temperatures, but we got by with antistatic mats, higher humidity and the polonium-210. We avoided using gloves as much as possible, using fine metal forceps for handling filters (I don't think we bothered with 'antistatic' forceps). Antistatic gloves weren't much use as we needed disposable ones. We used very thin sensitive clear plastic gloves that were probably something like Polyethylene, although they probably did have some static problems. Our Nuclepore filter samples were generally glassfibres recovered from lungs (fibre durability + toxicology studies) or aerosol particles, and obviously when using aqueous samples static was only a problem with the clean filter prior to filtration or after drying.
Regards
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
----- Original Message ----- X-from: {TindallR-at-missouri.edu} To: {keith.morris-at-ucl.ac.uk} Sent: Thursday, January 12, 2006 11:04 PM
I'll send you a PDF of the salary survey article that appeared in Microscopy Today last year. Also, if you are going to be a microscopist (congratulations!) you should sign up for a free subscription to Microscopy Today at http://www.microscopy-today.com.
Ron Anderson, Editor
dustin.adams-at-xerox.com wrote:
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==============================Original Headers============================== 6, 20 -- From microscopytoday-at-tampabay.rr.com Fri Jan 13 08:32:19 2006 6, 20 -- Received: from ms-smtp-03.tampabay.rr.com (ms-smtp-03-smtplb.tampabay.rr.com [65.32.5.133]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DEWIuY013660 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 13 Jan 2006 08:32:18 -0600 6, 20 -- Received: from [127.0.0.1] (rrcs-24-73-73-214.se.biz.rr.com [24.73.73.214]) 6, 20 -- by ms-smtp-03.tampabay.rr.com (8.13.4/8.13.4) with ESMTP id k0DEWDul022696; 6, 20 -- Fri, 13 Jan 2006 09:32:17 -0500 (EST) 6, 20 -- Message-ID: {43C7B9EC.8030201-at-tampabay.rr.com} 6, 20 -- Date: Fri, 13 Jan 2006 09:32:12 -0500 6, 20 -- From: Microscopy Today {microscopytoday-at-tampabay.rr.com} 6, 20 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 6, 20 -- X-Accept-Language: en-us, en 6, 20 -- MIME-Version: 1.0 6, 20 -- To: dustin.adams-at-xerox.com, Listserver {Microscopy-at-MSA.Microscopy.Com} 6, 20 -- Subject: Re: [Microscopy] AskAMicroscopist: SEM microscopist salaries 6, 20 -- References: {200601130224.k0D2Oc6V017874-at-ns.microscopy.com} 6, 20 -- In-Reply-To: {200601130224.k0D2Oc6V017874-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
Thanks for all the suggestions on static control! I learned about some new things to try for general use. I had forgotten about the polonium strip StaticMaster brushes from my days locked in a photo darkroom, but this may be a good alternative to these outrageously overpriced Zero Stat guns that don't work nearly as well as they did a few years ago.
Those StaticMaster brushes used to strike fear into us when we read the disposal instructions, which were something like "seal this strip inside a deep, deep mine and don't go anywhere near it for 5000 years", although we could use them in the darkroom with no special protection. They did work, though, except you couldn't spark your co-workers with them and make them squeal.
By the way, our most common use for these guns is somewhat trivial: getting grids down off the lid of the petri dish, where static electricity glues them, especially in winter. (Tamara, I agree with you about the static in New Mexico---I still have scars from getting zapped every time I'd get out of my car in Las Cruces!). We use the Diatome de-ionizer for microtomy, which works well.
Thanks again!
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 11, 23 -- From TindallR-at-missouri.edu Fri Jan 13 08:50:02 2006 11, 23 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49]) 11, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DEo1kl023115 11, 23 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 08:50:01 -0600 11, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 11, 23 -- Fri, 13 Jan 2006 08:49:59 -0600 11, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 23 -- Content-class: urn:content-classes:message 11, 23 -- MIME-Version: 1.0 11, 23 -- Content-Type: text/plain; 11, 23 -- charset="us-ascii" 11, 23 -- Subject: Static! 11, 23 -- Date: Fri, 13 Jan 2006 08:49:58 -0600 11, 23 -- Message-ID: {BA876152E8653240BE8572E897083EE701849CB9-at-UM-EMAIL09.um.umsystem.edu} 11, 23 -- X-MS-Has-Attach: 11, 23 -- X-MS-TNEF-Correlator: 11, 23 -- Thread-Topic: Static! 11, 23 -- Thread-Index: AcYYUKGubaVRxOz9TI+TEu+84a1Tdg== 11, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 11, 23 -- To: {microscopy-at-microscopy.com} 11, 23 -- X-OriginalArrivalTime: 13 Jan 2006 14:49:59.0341 (UTC) FILETIME=[A05B0DD0:01C61850] 11, 23 -- Content-Transfer-Encoding: 8bit 11, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0DEo1kl023115 ==============================End of - Headers==============================
You want static??!!! Try Manitoba in February. When it's -30C there's no such thing as moisture in the air. But then, not even Phil was willing to try Manitoba in June/July, even for real beer ;-) .
Great time to make formvar grids, though.
paul
} Paul R. Hazelton, PhD } Electron Microscope Unit } University of Manitoba } Department of Medical Microbiology } 531 Basic Medical Sciences Building } 730 William Avenue } Winnipeg, Manitoba, Canada, R3E 0W3 } e-mail: paul_hazelton-at-umanitoba.ca } Phone:204-789-3313 } Pager:204-931-9354 } Cell:204-781-1502 } Fax:204-789-3926
==============================Original Headers============================== 12, 20 -- From paul_hazelton-at-umanitoba.ca Fri Jan 13 09:59:12 2006 12, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 12, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DFwxMP001111 12, 20 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 09:59:01 -0600 12, 20 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 12, 20 -- (authenticated bits=0) 12, 20 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id k0DFwtYb015625; 12, 20 -- Fri, 13 Jan 2006 09:58:56 -0600 (CST) 12, 20 -- Message-ID: {43C7CE3B.4080709-at-umanitoba.ca} 12, 20 -- Date: Fri, 13 Jan 2006 09:58:51 -0600 12, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 12, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 12, 20 -- X-Accept-Language: en-us, en 12, 20 -- MIME-Version: 1.0 12, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 12, 20 -- Subject: Re: [Microscopy] Static! 12, 20 -- References: {200601131453.k0DErNj8026940-at-ns.microscopy.com} 12, 20 -- In-Reply-To: {200601131453.k0DErNj8026940-at-ns.microscopy.com} 12, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed 12, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Definition of milli-second is the amount of time between hitting send and realizing there is an error, da? Of course I meant phospholipid, not lipoprotein when mentioning the outer and cytoplasmic membranes. Mark it up to trying to do e-mail when the brain is turned off. Should Ron Anderson decide that this is a train worth following for Microscopy Today and want to use my response to you, perhaps he could make the correction, please??!!
Paul
} Paul R. Hazelton, PhD } Electron Microscope Unit } University of Manitoba } Department of Medical Microbiology } 531 Basic Medical Sciences Building } 730 William Avenue } Winnipeg, Manitoba, Canada, R3E 0W3 } e-mail: paul_hazelton-at-umanitoba.ca } Phone:204-789-3313 } Pager:204-931-9354 } Cell:204-781-1502 } Fax:204-789-3926
==============================Original Headers============================== 10, 20 -- From paul_hazelton-at-umanitoba.ca Fri Jan 13 09:59:41 2006 10, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 10, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DFxdMb001278 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 09:59:40 -0600 10, 20 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 10, 20 -- (authenticated bits=0) 10, 20 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id k0DFxcsx015957 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 09:59:38 -0600 (CST) 10, 20 -- Message-ID: {43C7CE65.7000006-at-umanitoba.ca} 10, 20 -- Date: Fri, 13 Jan 2006 09:59:33 -0600 10, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 10, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 10, 20 -- X-Accept-Language: en-us, en 10, 20 -- MIME-Version: 1.0 10, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 10, 20 -- Subject: Re: [Microscopy] bacterial permeabilization - further 10, 20 -- References: {200601130008.k0D08WwZ005845-at-ns.microscopy.com} 10, 20 -- In-Reply-To: {200601130008.k0D08WwZ005845-at-ns.microscopy.com} 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed 10, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Very interesting question. Made more interesting to me by the fact that I was discussing this same topic this afternoon, only more generically, and certainly not in reference to H. influenza. The problem you are faced with is bacterial structure. H. influenza is a Gram negative microorganism. Gram negative cells have two lipoprotein layers - the outer and cytoplasmic membranes. In addition, you have a layer of peptidoglycan between the outer and cytoplasmic membranes. To make it even more fun, there is frequently a lypopolysaccharide layer around the whole thing. You have to penetrate the LPS, permeabilize the outer membrane, traverse the periplasmic space (the volume between the outer and cytoplasmic membranes), cross the peptidoglycan layer and then penetrate the inner membrane, all with gentle treatment to allow the membranes to reorganize after removal of the detergent you use for permeabilization. What it adds up to is that permeabilization will be difficult at best, and most likely impossible. Probably the only way to get something reliably into the cytoplasm would be by active transport.
Having said that, knowing what I do about the structure of prokaryotic cells, I confess to having tried to permeabilize and do pre-embedding labeling on E. coli expressing a protein of interest (another gram negative bacterium). Didn't expect it, but tried anyway. Hey, dogma schmogma, give it a try, right? That's how the serendipitous findings occur. Anyway, used triton X, used saponin. Ugly. Did not look good. No luck either way. So logic and dogma were right this time.
At the same time I did LR white embedding on cells from the same broth culture. Included osmium fixation. Went back with metaperiodate followed by hydrogen peroxide etching and then did indirect IEM with 12nm gold for the label. Beautiful preservation. Got highly significant labeling of the expressed protein in the E. coli and in the wild type bacterium from which the protein of interest had been cloned. In fact, the protein of interest was hypothesized to be membrane inserted on the basis of amino acid sequence and the labeling of the wild type cells was primarily associated with the cytoplasmic membrane. In short, I did minor modification to the standard LR white embedding protocol and it worked great. I would give that a try.
I would like to give a reference for the work, but we are still waiting for the student to finish his thesis, not to mention the paper. And he has already left for his post-doc. If you have any questions, call. I would be glad to discuss the procedure I used further.
Paul
==============================Original Headers============================== 9, 21 -- From paul_hazelton-at-umanitoba.ca Fri Jan 13 10:12:07 2006 9, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 9, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DGC78e019513 9, 21 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 10:12:07 -0600 9, 21 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 9, 21 -- (authenticated bits=0) 9, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id k0DGC684025968; 9, 21 -- Fri, 13 Jan 2006 10:12:06 -0600 (CST) 9, 21 -- Message-ID: {43C7D151.2010503-at-umanitoba.ca} 9, 21 -- Date: Fri, 13 Jan 2006 10:12:01 -0600 9, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 9, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 9, 21 -- X-Accept-Language: en-us, en 9, 21 -- MIME-Version: 1.0 9, 21 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 9, 21 -- Subject: Re: [Microscopy] bacterial permeabilization 9, 21 -- References: {200601130008.k0D08WwZ005845-at-ns.microscopy.com} 9, 21 -- In-Reply-To: {200601130008.k0D08WwZ005845-at-ns.microscopy.com} 9, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0DGC78e019513 ==============================End of - Headers==============================
Hey! I would've been happy to go to Manitoba, even for real beer. Just kind of hard when I was busy fending off predatory administraitors. Gave up on that and just moved.
Phil
Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
Hi, Dustin
We did a salary survey for the society in conjunction with Microscopy Today late last year. Contact Ron Anderson for details. (see email above).
Hope this is helpful, Barbara Foster
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
P. S. Need a good general reference or light microscopy text for the Spring semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 08:25 PM 1/12/2006, dustin.adams-at-xerox.com wrote:
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==============================Original Headers============================== 13, 18 -- From bfoster-at-mme1.com Fri Jan 13 10:57:55 2006 13, 18 -- Received: from 5starpro.com (enterprise.5starpro.com [207.44.136.95]) 13, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DGvtFJ005902 13, 18 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 10:57:55 -0600 13, 18 -- Received: (qmail 27399 invoked from network); 13 Jan 2006 11:59:33 -0500 13, 18 -- Received: from host57-99.rancor.birch.net (HELO barbsd505.mme1.com) (65.17.57.99) 13, 18 -- by enterprise.5starpro.com with SMTP; 13 Jan 2006 11:59:33 -0500 13, 18 -- Message-Id: {6.1.2.0.0.20060113105646.0273a880-at-mail.mme1.com} 13, 18 -- X-Sender: bfoster-at-mail.mme1.com 13, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 6.1.2.0 13, 18 -- Date: Fri, 13 Jan 2006 10:57:56 -0600 13, 18 -- To: dustin.adams-at-xerox.com, microscopy-at-microscopy.com 13, 18 -- From: Barbara Foster {bfoster-at-mme1.com} 13, 18 -- Subject: Re: [Microscopy] AskAMicroscopist: SEM microscopist salaries 13, 18 -- In-Reply-To: {200601130225.k0D2PxKo021933-at-ns.microscopy.com} 13, 18 -- References: {200601130225.k0D2PxKo021933-at-ns.microscopy.com} 13, 18 -- Mime-Version: 1.0 13, 18 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
At 08:25 PM 1/12/2006, dustin.adams-at-xerox.com wrote:
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==============================Original Headers============================== 6, 18 -- From bfoster-at-mme1.com Fri Jan 13 11:03:21 2006 6, 18 -- Received: from 5starpro.com (enterprise.5starpro.com [207.44.136.95]) 6, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DH3KI6015095 6, 18 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 11:03:20 -0600 6, 18 -- Received: (qmail 27830 invoked from network); 13 Jan 2006 12:04:58 -0500 6, 18 -- Received: from host57-99.rancor.birch.net (HELO barbsd505.mme1.com) (65.17.57.99) 6, 18 -- by enterprise.5starpro.com with SMTP; 13 Jan 2006 12:04:58 -0500 6, 18 -- Message-Id: {6.1.2.0.0.20060113105606.026f6358-at-mail.mme1.com} 6, 18 -- X-Sender: bfoster-at-mail.mme1.com 6, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 6.1.2.0 6, 18 -- Date: Fri, 13 Jan 2006 11:03:21 -0600 6, 18 -- To: dustin.adams-at-xerox.com, microscopy-at-microscopy.com 6, 18 -- From: Barbara Foster {bfoster-at-mme1.com} 6, 18 -- Subject: Re: [Microscopy] AskAMicroscopist: SEM microscopist salaries 6, 18 -- In-Reply-To: {200601130225.k0D2PxKo021933-at-ns.microscopy.com} 6, 18 -- References: {200601130225.k0D2PxKo021933-at-ns.microscopy.com} 6, 18 -- Mime-Version: 1.0 6, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I am looking at getting a deeply cooled (-30 C) high sensitivity, high resolution digital camera. I am considering the following cameras : Hamamatsu Orca-AG Retiga SRV Photometrics CoolSnap HQ or K4
Any comments (public or private) on your experiences would be appreciated.
One source has told me that they see little practical difference in the Orca (cooled to -30) vs the Retiga EXi (cooled to 25 below ambient). Comments about this statement are also welcome.
Thanks, Tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
McCrone Associates, Inc., an innovative modern analytical and research laboratory, seeks a highly motivated electron microscopy technician with training in ultramicrotomy for materials science. The successful candidate will support our Electron Optics Group in sample preparation for SEM and TEM, as well as instrument maintenance. Ability to carry out routine SEM and TEM imaging and x-ray analysis is also desired. For more information on The McCrone Group, please visit www.mccrone.com.
The ideal candidate will have a B.S. degree with coursework in electron microscopy and microtomy, or comparable experience. Compensation is commensurate with education, experience and responsibilities.
Applicant selected must be a U.S. Citizen, will be subject to a government security investigation, and must meet eligibility requirements for access to classified information. McCrone is an Equal Opportunity Employer.
If you have microtomy experience and are looking for an interesting and unique career opportunity in materials analysis, send your resume with a letter describing your experience and interests to:
Careers McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, Illinois 60559
FAX: 630-887-7417 - Attn: Human Resources E-mail: careers-at-mccrone.com (e-mail is preferred)
Elaine Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 11, 27 -- From eschumacher-at-mccrone.com Fri Jan 13 12:14:59 2006 11, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122]) 11, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DIEwln001747 11, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 13 Jan 2006 12:14:59 -0600 11, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 11, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id DA9E61A800B 11, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 13 Jan 2006 12:14:58 -0600 (CST) 11, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 11, 27 -- by pgp.mccrone.com (PGP Universal service); 11, 27 -- Fri, 13 Jan 2006 12:14:58 -0600 11, 27 -- X-PGP-Universal: processed 11, 27 -- Content-class: urn:content-classes:message 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- charset="US-ASCII" 11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 11, 27 -- Subject: Open Position: Electron Microscopy Technician 11, 27 -- Date: Fri, 13 Jan 2006 12:14:48 -0600 11, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A74C3103-at-MCCRONEMSG.tmg.mccrone.com} 11, 27 -- X-MS-Has-Attach: 11, 27 -- X-MS-TNEF-Correlator: 11, 27 -- Thread-Topic: Open Position: Electron Microscopy Technician 11, 27 -- Thread-Index: AcYYbT0QYMn4lwyUQBa7kWOedjOuMA== 11, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 11, 27 -- To: {Microscopy-at-MSA.Microscopy.Com} 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0DIEwln001747 ==============================End of - Headers==============================
What you are seeing is the projected image of the hot filament (light). This means that although you have a hole down the column the electron gun is out of alignment. May I suggest the following.
At no more than 60kV switch off all the lenses and look for a very bright electron beam by adjusting the gun alignment controls. Once you find the beam switch each lens on one at a time, stepping back if the beam disappears.
Good luck
Steve Chapman Senior Consultant Protrain For electron microscopy consultancy and training world wide Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7802 966067 Web www.emcourses.com
----- Original Message ----- X-from: {aetmicro-at-optonline.net} To: {protrain-at-emcourses.com} Sent: Friday, January 13, 2006 2:22 AM
Group,
Our University in an effort of conservation of energy is making changes as to the HVAC conditions they can provide us. Requests as to equipment shuts downs during non use hours, shuting off computers every time not in use, and increasing lab temperatures beyound 78F are being asked for. Looking for documented case studies pro or con and anyones experience they would be willing to share offline on this issue. Would also like to hear from vendors as to desired environments for SEM and related equipment as well as stored chemicals.
Thanks
Roy Beavers
Southern Methodist University Department of Geological Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 7, 20 -- From rbeavers-at-mail.smu.edu Fri Jan 13 15:12:52 2006 7, 20 -- Received: from s31xe5.systems.smu.edu (s31xe5.systems.smu.edu [129.119.70.74]) 7, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DLCpEo022529 7, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 15:12:51 -0600 7, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 7, 20 -- Content-class: urn:content-classes:message 7, 20 -- MIME-Version: 1.0 7, 20 -- Content-Type: text/plain; 7, 20 -- charset="iso-8859-1" 7, 20 -- Subject: Lab Facilities Temperature 7, 20 -- Date: Fri, 13 Jan 2006 15:12:51 -0600 7, 20 -- Message-ID: {3A9F6E299461A44483961A57175598A703402D0F-at-s31xe5.systems.smu.edu} 7, 20 -- X-MS-Has-Attach: 7, 20 -- X-MS-TNEF-Correlator: 7, 20 -- Thread-Topic: Lab Facilities Temperature 7, 20 -- Thread-Index: AcYYhhysHdUwLzATSNSFiQlctfoZlA== 7, 20 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 7, 20 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0DLCpEo022529 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mswift-at-bunham.org) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, January 13, 2006 at 18:18:43 ---------------------------------------------------------------------------
Email: mswift-at-bunham.org Name: Mark Swift
Organization: Burnham Institute
Education: Graduate College
Location: San Diego, CA
Question: We have a Tecnai 12 from FEI, and we would like to know: How do we ensure that in Low Dose our Focus Substates 1 and 2 are on the tilt axis when Alpha angle is not 0 degrees?
I am seeking advice on obtaining a low cost polisher for SEM sample preparation. The samples will be coated thin metal cross-sections embedded in epoxy. The cross-sections will be analyzed by SEM to determine coating thickness. I have a diamond wet saw but I need a polisher but have very little funds available. I found a used Buehler Minimet polisher in my price range ~$1K. I am not familiar with this unit. I have used bigger units like the Buehler Powerpro and the Leco SS-2000. Is the difference mainly a matter of speed and sample size? I plan on doing only about one sample a week. Any advice will be appreciated. Thanks,
Scott LeMaster PPG Industries Inc. lemaster-at-ppg.com
==============================Original Headers============================== 4, 23 -- From lemaster-at-ppg.com Mon Jan 16 09:31:25 2006 4, 23 -- Received: from SGOFSMTP02.nac.ppg.com (smtpout.ppg.com [141.189.251.4]) 4, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0GFVMxY018188 4, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jan 2006 09:31:24 -0600 4, 23 -- Received: from SGOFUSR24.nac.ppg.com ([10.49.120.133]) by SGOFSMTP02.nac.ppg.com with Microsoft SMTPSVC(5.0.2195.6713); 4, 23 -- Mon, 16 Jan 2006 10:31:20 -0500 4, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 4, 23 -- Content-Class: urn:content-classes:message 4, 23 -- MIME-Version: 1.0 4, 23 -- Content-Type: text/plain; 4, 23 -- charset="us-ascii" 4, 23 -- Subject: SEM advice on low cost polisher 4, 23 -- Date: Mon, 16 Jan 2006 10:31:20 -0500 4, 23 -- Message-ID: {B92A132E126C4E45B3AAC00B5623D3E7015AF8CA-at-sgofusr24.nac.ppg.com} 4, 23 -- X-MS-Has-Attach: 4, 23 -- X-MS-TNEF-Correlator: 4, 23 -- Thread-Topic: SEM advice on low cost polisher 4, 23 -- thread-index: AcYaseX2g8/b/IiKQ069aQDRzXKGcA== 4, 23 -- From: "LeMaster, J. Scott" {lemaster-at-ppg.com} 4, 23 -- To: {Microscopy-at-microscopy.com} 4, 23 -- X-OriginalArrivalTime: 16 Jan 2006 15:31:20.0666 (UTC) FILETIME=[E69473A0:01C61AB1] 4, 23 -- Content-Transfer-Encoding: 8bit 4, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0GFVMxY018188 ==============================End of - Headers==============================
Scott: on the MiniMet, the sample moves and the polishing media stays still. This tool can take up to a 2 inch potted sample and uses 4-inch PSA grinding/polishing media on glass discs. It was designed to do rough and fine polish and it does that pretty well. On this tool, you drill a small blind hole in the top of your sample. This is where a spring-loaded arm fits to move the sample in an random orbital motion. You have some control over the pressure of the sample into the media and the speed of rotation. It can run unattended. This tool might be just what you need for one sample a week, if you can cut almost to the area of interest and then polish the rest of the way. On used tools, the spring force is usually the first thing to go due to rust, so be aware.
lemaster-at-ppg.com wrote:
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-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 5, 23 -- From r-holdford-at-ti.com Mon Jan 16 10:43:49 2006 5, 23 -- Received: from reloaded.ext.ti.com (reloaded.ext.ti.com [192.94.94.6]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0GGhmBh028324 5, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 16 Jan 2006 10:43:48 -0600 5, 23 -- Received: from dlep30.itg.ti.com ([157.170.170.32]) 5, 23 -- by reloaded.ext.ti.com (8.13.4/8.13.4) with ESMTP id k0GGh3mi021873; 5, 23 -- Mon, 16 Jan 2006 10:43:32 -0600 (CST) 5, 23 -- Received: from [156.117.194.120] (localhost [127.0.0.1]) 5, 23 -- by dlep30.itg.ti.com (8.12.11/8.12.11) with ESMTP id k0GGf6pY012416; 5, 23 -- Mon, 16 Jan 2006 10:41:07 -0600 (CST) 5, 23 -- Message-ID: {43CBCCA2.2060902-at-ti.com} 5, 23 -- Date: Mon, 16 Jan 2006 10:41:06 -0600 5, 23 -- From: Becky Holdford {r-holdford-at-ti.com} 5, 23 -- Organization: SC Packaging Development -- FA Development 5, 23 -- User-Agent: Mozilla Thunderbird 0.8 (Windows/20040913) 5, 23 -- X-Accept-Language: en-us, en 5, 23 -- MIME-Version: 1.0 5, 23 -- To: lemaster-at-ppg.com, MSA ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 23 -- Subject: Re: [Microscopy] SEM advice on low cost polisher 5, 23 -- References: {200601161533.k0GFXXfg018559-at-ns.microscopy.com} 5, 23 -- In-Reply-To: {200601161533.k0GFXXfg018559-at-ns.microscopy.com} 5, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The College of Microscopy will be offering a short course, Scanning Electron Microscopy, March 27-31, 2006, at our Westmont Facility. In addition to lectures, the course emphasizes hands-on training using five scanning electron microscopes and electron microprobe analyzers, and gives students the opportunity to work on their own samples. For further details and registration information, please follow the link below.
www.collegeofmicroscopy.com
Elaine Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 8, 27 -- From eschumacher-at-mccrone.com Mon Jan 16 11:56:32 2006 8, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122]) 8, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0GHuW0p005961 8, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 16 Jan 2006 11:56:32 -0600 8, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 8, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 797C11A800B 8, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 16 Jan 2006 11:56:34 -0600 (CST) 8, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 8, 27 -- by pgp.mccrone.com (PGP Universal service); 8, 27 -- Mon, 16 Jan 2006 11:56:34 -0600 8, 27 -- X-PGP-Universal: processed 8, 27 -- MIME-Version: 1.0 8, 27 -- Content-Type: text/plain; 8, 27 -- charset="US-ASCII" 8, 27 -- Content-class: urn:content-classes:message 8, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 8, 27 -- Subject: Short Course Announcement: Scanning Electron Microscopy 8, 27 -- Date: Mon, 16 Jan 2006 11:56:21 -0600 8, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A74C310A-at-MCCRONEMSG.tmg.mccrone.com} 8, 27 -- X-MS-Has-Attach: 8, 27 -- X-MS-TNEF-Correlator: 8, 27 -- Thread-Topic: Short Course Announcement: Scanning Electron Microscopy 8, 27 -- thread-index: AcYaxijJDPWYg/gFR2u07q3UCQ75Vw== 8, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 8, 27 -- To: {Microscopy-at-MSA.Microscopy.Com} 8, 27 -- Content-Transfer-Encoding: 8bit 8, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0GHuW0p005961 ==============================End of - Headers==============================
DISCLAIMER: South Bay Technology produces equipment and supplies as described below and, therefore, has a vested interest in promoting their use.
If you are mounting the sample in AcryliMet™ cold mount or something similar and can hold your sample by hand, then something like our Model 900 Grinder/Polisher would be ideal. A new Model 900 falls into about the same price range as the used Minimet you referred to. This system is often used for low volume polishing requirements - although usually for higher volume than just 1 sample per week. If you have unencapsulated samples or simply want more control over the polishing process, you can use the Model 900 together with something like our Tripod Polisher® Cross Section Polisher, our BiPod™ Cross Section Polisher or one of our other lapping and polishing fixtures. You can find information on the Model 900 Polisher at http://southbaytech.com/products_index.cfm?main_action=product_detail&ProductID=42.
If you do end up with the Minimet Polisher, I do have a large lot of Minimet Consumables available at a huge discount. If you have an interest in those, please let me know and I will send you the listing.
Best regards-
David
lemaster-at-ppg.com wrote:
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==============================Original Headers============================== 20, 21 -- From henriks-at-southbaytech.com Mon Jan 16 13:06:35 2006 20, 21 -- Received: from ylpvm25.prodigy.net (ylpvm25-ext.prodigy.net [207.115.57.56]) 20, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0GJ6ZDe016034 20, 21 -- for {microscopy-at-msa.microscopy.com} ; Mon, 16 Jan 2006 13:06:35 -0600 20, 21 -- Received: from southbaytech.com (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90]) 20, 21 -- (authenticated bits=0) 20, 21 -- by ylpvm25.prodigy.net (smtpauth/dk/flock 8.13.4/8.13.4) with ESMTP id k0GJ68Ik027256; 20, 21 -- Mon, 16 Jan 2006 14:06:10 -0500 20, 21 -- Message-ID: {43CBEEC8.3020505-at-southbaytech.com} 20, 21 -- Date: Mon, 16 Jan 2006 11:06:48 -0800 20, 21 -- From: David Henriks {henriks-at-southbaytech.com} 20, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 20, 21 -- X-Accept-Language: en-us, en 20, 21 -- MIME-Version: 1.0 20, 21 -- To: lemaster-at-ppg.com 20, 21 -- CC: Microscopy Listerver {microscopy-at-msa.microscopy.com} 20, 21 -- Subject: Re: [Microscopy] SEM advice on low cost polisher 20, 21 -- References: {200601161548.k0GFmtuG021248-at-ns.microscopy.com} 20, 21 -- In-Reply-To: {200601161548.k0GFmtuG021248-at-ns.microscopy.com} 20, 21 -- Content-Type: text/plain; charset=windows-1252; format=flowed 20, 21 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I just returned from a trip out of the country, and found an inquiry about starting up ion pumps in among 1200 other assorted e-mail messages (mostly spam), and so I apologize for being so delayed in answering.
I don't know the exact details of the arrangement of the vacuum system on a JEOL 2010, but in general there should be no requirement for having cooling water running while starting up or running a sputter ion pump. In fact, one of the principle advantages of these pumps is the fact that they do not need cooling of any sort for normal operation.
What you would need to do is to set the valves in the vacuum system so that the backing pump evacuates the region of the vacuum system served by the ion pumps, then evacuate this region to a vacuum in the low 10-3 torr range. Then, follow the start-up procedure recommended for your ion pumps. You want to minimize the time the backing pumps are operated in the low end of this pressure range to reduce the possibility of contaminating the system by back streaming of oil from them, otherwise, there should be no problem starting up the ion pumps. As soon as they do start you want to close whatever valves are available to isolate the part of the system they serve from the part evacuated by the other pumps. Once the supply of cooling water is restored, use the diffusion pump to evacuate the rest of the system to an operating level, and then open the valves to the ion-pumped region and resume normal operation.
If you can get hold of a copy of my book on Vacuum Methods in Electron Microscopy (ISBN 1 85578 053 4) you can find the matter of backstreaming from rotary vane pumps discussed in Section 4.1.5 on p. 144. The general operating characteristics of sputter ion pumps is discussed in Sections 7.1.6, p 290 and 7.5, p. 322. I believe the operating procedure for the vacuum system described in Section 9.3 on p. 392 is very similar to that for the 2010.
Good luck, Wil Bigelow
-- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-engin.umich.edu; Fx:734-763-4788; Ph:734-662-5237 Address mail to: 1136 Mixtwood Rd. Ann Arbor, MI 48103-3035
==============================Original Headers============================== 7, 14 -- From bigelow-at-engin.umich.edu Mon Jan 16 14:34:37 2006 7, 14 -- Received: from srvr22.engin.umich.edu (srvr22.engin.umich.edu [141.213.75.21]) 7, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0GKYZh8026672 7, 14 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jan 2006 14:34:36 -0600 7, 14 -- Received: from [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 7, 14 -- by srvr22.engin.umich.edu (8.12.10/8.12.10) with ESMTP id k0GKYYGU013312 7, 14 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jan 2006 15:34:34 -0500 (EST) 7, 14 -- Mime-Version: 1.0 7, 14 -- Message-Id: {p06210201bff1b09494cf-at-[141.212.131.221]} 7, 14 -- Date: Mon, 16 Jan 2006 15:34:33 -0500 7, 14 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 7, 14 -- From: Wil Bigelow {bigelow-at-engin.umich.edu} 7, 14 -- Subject: RE:[Microscopy] Starting Ion Pumps without cooling water 7, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I too am not aware of any water cooling for ion pumps. when first pumping, they do get warm. But their controller will shut them off for a cool down.
The main problem I have had in the past is getting them to start pumping. Initially, no pump current. This is fixed by heating the pump with a hair dryer. Then they fire and that is that. This is typical of the little 1-5L/m gun chamber pumps for LaB6. The larger 30L/m pumps don't seem to have this problem. This was with Varian pumps.
gary g.
At 12:53 PM 1/16/2006, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Mon Jan 16 15:40:09 2006 11, 20 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k0GLe9od004433 11, 20 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jan 2006 15:40:09 -0600 11, 20 -- Received: (qmail 5325 invoked from network); 16 Jan 2006 13:40:08 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 5322, pid: 5323, t: 0.1787s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 20 -- by qsmtp2 with SMTP; 16 Jan 2006 13:40:08 -0800 11, 20 -- Message-Id: {6.2.3.4.2.20060116133653.0206f088-at-mail.calweb.com} 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 11, 20 -- Date: Mon, 16 Jan 2006 13:40:08 -0800 11, 20 -- To: bigelow-at-engin.umich.edu 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] RE: Starting Ion Pumps without cooling water 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200601162053.k0GKrXr6030172-at-ns.microscopy.com} 11, 20 -- References: {200601162053.k0GKrXr6030172-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I have a multi-function gauge on my T300 that reads from 0-1. It is used with a rotating switch to measure all the voltages etc in the system. When its at full vacuum it reads 0.42 and the manual says it should be 6v which I assume is 0.6. I was wondering if anyone out there knows what this translates to in terms of Torr? Please reply off list if this is not of general interest.
Thanks in advance!
Tom Kaye
==============================Original Headers============================== 5, 20 -- From tom-at-tomkaye.com Mon Jan 16 20:44:11 2006 5, 20 -- Received: from tomkaye.com (mxgateway.techpro.com [64.4.207.8]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0H2iAoG017269 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jan 2006 20:44:10 -0600 5, 20 -- Received: from tkdell [67.165.188.91] by tomkaye.com with ESMTP 5, 20 -- (SMTPD32-8.14) id A9F8638023E; Mon, 16 Jan 2006 20:44:08 -0600 5, 20 -- From: "Tom" {tom-at-tomkaye.com} 5, 20 -- To: {Microscopy-at-microscopy.com} 5, 20 -- Subject: Question about vacuum gauge on JEOL T300 5, 20 -- Date: Mon, 16 Jan 2006 20:44:09 -0600 5, 20 -- Message-ID: {021901c61b0f$e4b081b0$030a1aac-at-tkdell} 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; 5, 20 -- charset="iso-8859-1" 5, 20 -- Content-Transfer-Encoding: 7bit 5, 20 -- X-Priority: 3 (Normal) 5, 20 -- X-MSMail-Priority: Normal 5, 20 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2910.0) 5, 20 -- Importance: Normal 5, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
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Email: cumings-at-umd.edu Name: John Cumings
Organization: University of Maryland
Title-Subject: [Filtered] Job Opening
Question: Dear Microscopy Community,
I would like to bring your attention to an open position in electron microscopy as the lab manager of a newly-created facility here at the University of Maryland. The position is part of the growing nanoscience center on campus, the Maryland Center for Integrated Nano Science and Engineering (M-CINSE).
More information can be found at http://mse.umd.edu/dept/positions/LabManagerUniversityMicroscopyFacility.htm
and applications can be submitted at https://apra.umd.edu/search.jsp?ID=ENMA000003
Please note that the "best consideration" deadline of Jan. 15th has just passed, so please apply soon if you are interested.
Best regards,
-John Cumings
-- John Cumings cumings-at-umd.edu Assistant Professor Department of Materials Science and Engineering University of Maryland College Park, MD 20742-2115
office (301) 405-0789 (1246 Kim Building) fax (301) 314-8164 --
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Email: nrvrctr-at-adelphia.net Name: Ed Beckett
Organization: MRI Virginia
Title-Subject: [Filtered] SEM
Question: I am searching for a Test engineer in Chicago with experience in SEM as well as other electrical and test abilities. Salary is about $60K. Contact me if you are aware of anyone or self interested. Thanks Ed Beckett 540-980-3100
I am interested to study magnetic structure (type II magnetic contrast) by SEM. Our SEM equipped with a Four Quadrant Backscattered Electron Detector. I will appreciate if you have experience using this type of detector for magnetic domain structure to guide us with the recommended working parameters as combinations of quadrant setting, working distance, tilt and etc.
Thank you in advanced,
Yossi.
==============================Original Headers============================== 5, 30 -- From yezer-at-cc.hut.fi Tue Jan 17 02:22:24 2006 5, 30 -- Received: from smtp-3.hut.fi (smtp-3.hut.fi [130.233.228.93]) 5, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0H8MN4R016682 5, 30 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Jan 2006 02:22:24 -0600 5, 30 -- Received: from localhost (putosiko.hut.fi [130.233.228.114]) 5, 30 -- by smtp-3.hut.fi (8.12.10/8.12.10) with ESMTP id k0H8MM6h013963 5, 30 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Jan 2006 10:22:22 +0200 5, 30 -- Received: from smtp-3.hut.fi ([130.233.228.93]) 5, 30 -- by localhost (putosiko.hut.fi [130.233.228.114]) (amavisd-new, port 10024) 5, 30 -- with LMTP id 22195-17-9 for {Microscopy-at-Microscopy.Com} ; 5, 30 -- Tue, 17 Jan 2006 10:22:22 +0200 (EET) 5, 30 -- Received: from baresi.hut.fi (baresi-m.hut.fi [130.233.228.121]) 5, 30 -- by smtp-3.hut.fi (8.12.10/8.12.10) with ESMTP id k0H8LuRu013837 5, 30 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Jan 2006 10:21:56 +0200 5, 30 -- Received: (from apache-at-localhost) 5, 30 -- by baresi.hut.fi (8.12.10/8.12.6/Submit) id k0H8LtkJ029142 5, 30 -- for Microscopy-at-Microscopy.Com; Tue, 17 Jan 2006 10:21:55 +0200 5, 30 -- To: Microscopy-at-Microscopy.Com 5, 30 -- Subject: Magnetic contrast with 4QBSED 5, 30 -- Message-ID: {1137486115.43cca9239de7c-at-webmail1.hut.fi} 5, 30 -- Date: Tue, 17 Jan 2006 10:21:55 +0200 (EET) 5, 30 -- From: yezer-at-cc.hut.fi 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Type: text/plain; charset=ISO-8859-1 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- User-Agent: HUT webmail, IMP 2.2.6 5, 30 -- X-Authenticated-Sender: yezer-at-cc.hut.fi 5, 30 -- X-Originating-IP: 130.233.108.169 5, 30 -- X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on putosiko.hut.fi 5, 30 -- X-TKK-Virus-Scanned: by amavisd-new-2.1.2-hutcc at putosiko.hut.fi ==============================End of - Headers==============================
The Naval Research Laboratory has a current opening for a Postdoctoral Fellow in the Joining and Transformations Section. The ideal candidate would have a strong background in both electron microscopy and materials science. The successful candidate will study the transformations that occur during joining processes and relate the microstructural features to observed mechanical properties. Current research interests center on the precipitation, dynamic recrystallization, and texture evolution that occur during the friction stir welding process.
The Naval Research Laboratory has an excellent array of microscopy facilities available. There are three transmission electron microscopes (a Phillips CM-30 analytical TEM, a Hitachi H-9000 high-resolution TEM, and a new, state-of-the-art JEOL 2200 energy filtered STEM/TEM), two scanning electron microscopes (a Leo 1550 SEM with electron backscattered diffraction analysis and energy dispersive spectrometry and a Hitachi FEG-SEM), and a dual-beam focused ion beam with EBSD that are available for use by the successful candidate. Additional facilities that may be used include a quenching and deformation dilatometer, a Gleeble thermomechanical simulator, x-ray diffractometers, and an automated microhardness tester.
Please note that this position is only open to US citizens or Green Card holders.
Please contact me by e-mail (Fonda-at-anvil.nrl.navy.mil) or phone (202-767-2622) for further details about this research program.
Sinceerely,
Richard Fonda -- ________________________________________________________________ Richard W. Fonda Naval Research Laboratory (202) 767-2622 Code 6324 (202) 767-2623 fax Washington DC 20375 ________________________________________________________________
==============================Original Headers============================== 6, 14 -- From fonda-at-anvil.nrl.navy.mil Tue Jan 17 06:21:09 2006 6, 14 -- Received: from anvil.nrl.navy.mil (anvil.nrl.navy.mil [132.250.127.188]) 6, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HCL8kO029509 6, 14 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Jan 2006 06:21:09 -0600 6, 14 -- Received: from [132.250.127.172] (rw-fonda.nrl.navy.mil [132.250.127.172]) 6, 14 -- by anvil.nrl.navy.mil (8.12.11/8.12.11) with ESMTP id k0HCL5eT022874 6, 14 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Jan 2006 07:21:06 -0500 (EST) 6, 14 -- Mime-Version: 1.0 6, 14 -- Message-Id: {p06230901bff2920cbcd0-at-[132.250.127.172]} 6, 14 -- Date: Tue, 17 Jan 2006 07:22:41 -0500 6, 14 -- To: Microscopy-at-microscopy.com 6, 14 -- From: "Richard W. Fonda" {fonda-at-anvil.nrl.navy.mil} 6, 14 -- Subject: Postdoctoral Fellowship opening at NRL 6, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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Email: lleroux-at-csir.co.za Name: Lukas le Roux
Organization: CSIR
Title-Subject: [Filtered] Edwards Sputter Coater
Question: I am trying to find an instruction manual for a model S150B Sputter Coater. Is there a website from where I can download it?
Is there a company that can provide room survey services in the Dallas, Texas area? Environmental testing for mechanical vibration and stray EMF parameters are desired.
Please respond directly off list.
Bob Roberts EM Lab Services, Inc. 449 NW 62nd St. Topeka, Kansas 66617-1780 785.246.1232 voice 785.246.0168 fax www.emlabservices.com
==============================Original Headers============================== 6, 23 -- From emlabservices-at-cox.net Tue Jan 17 07:35:50 2006 6, 23 -- Received: from centrmmtao06.cox.net (centrmmtao06.cox.net [70.168.83.78]) 6, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HDZm75007409 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Jan 2006 07:35:49 -0600 6, 23 -- Received: from EMLabServices ([24.255.213.54]) by centrmmtao06.cox.net 6, 23 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with SMTP 6, 23 -- id {20060117133551.RBDG4002.centrmmtao06.cox.net-at-EMLabServices} 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Jan 2006 08:35:51 -0500 6, 23 -- Message-ID: {008601c61b6a$f0ad2440$6400a8c0-at-EMLabServices} 6, 23 -- From: "EM Lab Services" {emlabservices-at-cox.net} 6, 23 -- To: {Microscopy-at-microscopy.com} 6, 23 -- Subject: TEM Room Survey Company 6, 23 -- Date: Tue, 17 Jan 2006 06:35:53 -0700 6, 23 -- MIME-Version: 1.0 6, 23 -- Content-Type: text/plain; 6, 23 -- format=flowed; 6, 23 -- charset="iso-8859-1"; 6, 23 -- reply-type=original 6, 23 -- Content-Transfer-Encoding: 7bit 6, 23 -- X-Priority: 3 6, 23 -- X-MSMail-Priority: Normal 6, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 6, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
"I am discussing proposals to install an imaging system here. It is one that can capture both FISH and brown/ H&E stains and then allow an operator, using selected software, to analyse them. The favoured system at present is the Applied Imaging one as it is now undergoing a major facelift.
I am just wondering if there are any other systems on the market right now that can load up 50 slides automatically overnight, scan & store so that an operator can then analyse the images captured the following morning. None of the other major suppliers here in the UK, such as ChromaVision, Meta-systems & Aperio can offer both overnight loading and scanning of both FISH and brown/ blue & pink stains at present."
Gareth Morgan MPhil MSc FIBMS, Department of Histo/cytopathology, Laboratory Medicine (Labmed), Karolinska Institute, Karolinska University Hospital at Huddinge, F46 SE 141 86 Stockholm Sweden
OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet för klinisk patologi och cytologi.
NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10. Clinical Histo- and Cytopathology Laboratory.
==============================Original Headers============================== 14, 18 -- From Gareth.Morgan-at-ki.se Tue Jan 17 10:10:41 2006 14, 18 -- Received: from humle.it.ki.se (humle.it.ki.se [130.237.101.252]) 14, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HGAeFM027877 14, 18 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2006 10:10:41 -0600 14, 18 -- Received: from lgtestdator.ki.se (hsg01.hs.se [193.10.76.5]) 14, 18 -- by humle.it.ki.se (8.13.1/8.13.1) with ESMTP id k0HGAeo4003655 14, 18 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2006 17:10:40 +0100 (MET) 14, 18 -- Message-Id: {7.0.0.16.0.20060117171215.020160a8-at-ki.se} 14, 18 -- Message-Id: {7.0.0.16.0.20060116170224.020ac968-at-ki.se} 14, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.0.16 14, 18 -- Date: Tue, 17 Jan 2006 17:12:17 +0100 14, 18 -- To: microscopy-at-microscopy.com 14, 18 -- From: Gareth Morgan {Gareth.Morgan-at-ki.se} 14, 18 -- Subject: Image analysis 14, 18 -- Mime-Version: 1.0 14, 18 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 14, 18 -- Content-Transfer-Encoding: 8bit 14, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0HGAeFM027877 ==============================End of - Headers==============================
We're having trouble with bright spots appearing on our tissue when viewing our grids in our new TEM. These spots may be described as bleaching, etching, burning or clearing. The bright spots appears after using high magnification to view an area or to focus. When we go back to a low magnification, we see the bright area.
This started happening with the installation of a new TEM. We haven't changed processing procedures and we are experienced instrument operators. The service engineer has determined that the TEM is working properly. The problem is not due to prolonged exposure to the electron beam. The spots occur within seconds. The filament is positioned correctly. Often there is variability in the intensity of the spotting even on the same grid. In other words, sometime the spot is very apparent and distinctive and other times it's more diffuse. We've tried using liquid nitrogen to improve the vacuum and adding a second 100% resin infiltration step to ensure removal of water from the specimen. The problem occurs with cells and tissues embedded in epoxy. The vendor has suggested working at 120kV and we're starting to do that now. It doesn't entirely correct the problem, but the spots are less focal.
Has anyone worked with a particular epoxy that stands up well or better than others under the electron beam? You can reply off list. Has anyone had this problem and been able find a solution?
Thank you in advance for your time
Marcia
Marcia Pitzenberger Pathology Laboratories Merck & Co., Inc. P.O. Box 4, WP45-104 West Point, PA 19486-0004 Tel 215 652-9767 Fax 215 652-7758 marcia_pitzenberger-at-merck.com
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==============================Original Headers============================== 13, 24 -- From marcia_pitzenberger-at-merck.com Tue Jan 17 11:12:37 2006 13, 24 -- Received: from usryim09.merck.com (taz.merck.com [155.91.6.20]) 13, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HHCaxL008406 13, 24 -- for {microscopy-at-msa.microscopy.com} ; Tue, 17 Jan 2006 11:12:36 -0600 13, 24 -- Received: from 155.91.2.6 by usryim09.merck.com with ESMTP (SMTP Relay); 13, 24 -- Tue, 17 Jan 2006 12:12:27 -0500 13, 24 -- X-Server-Uuid: 078441B8-8B94-4128-83AA-0F28411313D9 13, 24 -- Received: from 54.3.102.166 by USRYTW32.merck.com with ESMTP (Tumbleweed 13, 24 -- Email Firewall SMTP Relay (Email Firewall v6.1.1)); Tue, 17 Jan 2006 13, 24 -- 12:12:16 -0500 13, 24 -- X-Server-Uuid: 05354929-4888-4C60-B5F7-73C306CFF21D 13, 24 -- Received: by usrygw30.merck.com with Internet Mail Service (5.5.2658.27) 13, 24 -- id {C9J1FVY2} ; Tue, 17 Jan 2006 12:12:16 -0500 13, 24 -- Message-ID: {4F1089C79438304CB5A97E982A11AC1F05AF89-at-usctmx1118.merck.com} 13, 24 -- From: "Pitzenberger, Marcia H." {marcia_pitzenberger-at-merck.com} 13, 24 -- To: "'microscopy-at-msa.microscopy.com'" {microscopy-at-msa.microscopy.com} 13, 24 -- Subject: TEM - Electron Beam Clearing 13, 24 -- Date: Tue, 17 Jan 2006 12:12:07 -0500 13, 24 -- MIME-Version: 1.0 13, 24 -- X-Mailer: Internet Mail Service (5.5.2658.27) 13, 24 -- X-WSS-ID: 6FD3FAFA1X466977-01-01 13, 24 -- X-WSS-ID: 6FD3FAF12C419349-01-01 13, 24 -- Content-Type: text/plain 13, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
On Jan 14, 2006, at 5:35 AM, mswift-at-bunham.org wrote:
} Question: We have a Tecnai 12 from FEI, and we would like to know: How } do we ensure that in Low Dose our Focus Substates 1 and 2 are on the } tilt axis when Alpha angle is not 0 degrees? } Dear Mark, If you set the focus angles for the substates to 0 and 180 degrees, the displacements will be along the tilt axis. You can verify this by burning a small hole in the ice on a cryogrid in each of the focus substates, then going to a lower mag, where the holes are both visible in the exposure state, and tilting the grid. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Tue Jan 17 12:14:06 2006 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HIE5Ii019810 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 17 Jan 2006 12:14:05 -0600 4, 22 -- Received: from localhost (earth-dog [192.168.1.3]) 4, 22 -- by earth-ox-postvirus (Postfix) with ESMTP id 9B83B109E08 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 17 Jan 2006 10:14:03 -0800 (PST) 4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 4, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id B68A633C9D 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 17 Jan 2006 10:14:02 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v623) 4, 22 -- In-Reply-To: {200601141335.k0EDZ0gB029586-at-ns.microscopy.com} 4, 22 -- References: {200601141335.k0EDZ0gB029586-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {b7eeb9658bb9479e350b822656f74027-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] AskAMicroscopist: Tecnai 12 Focus Substates 4, 22 -- Date: Tue, 17 Jan 2006 10:20:58 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.623) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.3.3 ==============================End of - Headers==============================
I speak from a position of ignorance of your type of specimen, however I have picked up on 'This started happening with the installation of a new TEM'.
Are you using a much higher beam current at high mags than previously? Modern TEMs have much better condenser optics than the older ranges available in the 70s and 80s with less use of apertures to reduce spot size and better use of lenses. Have you changed from tungsten to LaB6 or FEG?
These changes can result in more electrons getting to the specimen. Can you compare the beam current that you use at high mag on the two TEMs? If you know typical exposure times of the two instruments do you have to spread the beam more on the new instument to get the same exposure time? Try using a smaller spot size or smaller condenser aperture and see if the effect improves.
Good luck, Ron
In message {200601171752.k0HHqH72018071-at-ns.microscopy.com} marcia_pitzenberger-at-merck.com writes: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } TEM User's - } } } We're having trouble with bright spots appearing on our tissue when viewing } our grids in our new TEM. These spots may be described as bleaching, } etching, burning or clearing. The bright spots appears after using high } magnification to view an area or to focus. When we go back to a low } magnification, we see the bright area. } } This started happening with the installation of a new TEM. We haven't } changed processing procedures and we are experienced instrument operators. } The service engineer has determined that the TEM is working properly. The } problem is not due to prolonged exposure to the electron beam. The spots } occur within seconds. The filament is positioned correctly. Often there is } variability in the intensity of the spotting even on the same grid. In } other words, sometime the spot is very apparent and distinctive and other } times it's more diffuse. We've tried using liquid nitrogen to improve the } vacuum and adding a second 100% resin infiltration step to ensure removal of } water from the specimen. The problem occurs with cells and tissues } embedded in epoxy. The vendor has suggested working at 120kV and we're } starting to do that now. It doesn't entirely correct the problem, but the } spots are less focal. } } Has anyone worked with a particular epoxy that stands up well or better than } others under the electron beam? You can reply off list. Has anyone had } this problem and been able find a solution? } } Thank you in advance for your time } } Marcia } } } } Marcia Pitzenberger } Pathology Laboratories } Merck & Co., Inc. } P.O. Box 4, WP45-104 } West Point, PA 19486-0004 } Tel 215 652-9767 } Fax 215 652-7758 } marcia_pitzenberger-at-merck.com } } } } } } ----------------------------------------------------------------------------- - } Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. } ----------------------------------------------------------------------------- - } } ==============================Original Headers============================== } 13, 24 -- From marcia_pitzenberger-at-merck.com Tue Jan 17 11:12:37 2006 } 13, 24 -- Received: from usryim09.merck.com (taz.merck.com [155.91.6.20]) } 13, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HHCaxL008406 } 13, 24 -- for {microscopy-at-msa.microscopy.com} ; Tue, 17 Jan 2006 11:12:36 -0600 } 13, 24 -- Received: from 155.91.2.6 by usryim09.merck.com with ESMTP (SMTP Relay); } 13, 24 -- Tue, 17 Jan 2006 12:12:27 -0500 } 13, 24 -- X-Server-Uuid: 078441B8-8B94-4128-83AA-0F28411313D9 } 13, 24 -- Received: from 54.3.102.166 by USRYTW32.merck.com with ESMTP (Tumbleweed } 13, 24 -- Email Firewall SMTP Relay (Email Firewall v6.1.1)); Tue, 17 Jan 2006 } 13, 24 -- 12:12:16 -0500 } 13, 24 -- X-Server-Uuid: 05354929-4888-4C60-B5F7-73C306CFF21D } 13, 24 -- Received: by usrygw30.merck.com with Internet Mail Service (5.5.2658.27) } 13, 24 -- id {C9J1FVY2} ; Tue, 17 Jan 2006 12:12:16 -0500 } 13, 24 -- Message-ID: {4F1089C79438304CB5A97E982A11AC1F05AF89-at-usctmx1118.merck.com} } 13, 24 -- From: "Pitzenberger, Marcia H." {marcia_pitzenberger-at-merck.com} } 13, 24 -- To: "'microscopy-at-msa.microscopy.com'" {microscopy-at-msa.microscopy.com} } 13, 24 -- Subject: TEM - Electron Beam Clearing } 13, 24 -- Date: Tue, 17 Jan 2006 12:12:07 -0500 } 13, 24 -- MIME-Version: 1.0 } 13, 24 -- X-Mailer: Internet Mail Service (5.5.2658.27) } 13, 24 -- X-WSS-ID: 6FD3FAFA1X466977-01-01 } 13, 24 -- X-WSS-ID: 6FD3FAF12C419349-01-01 } 13, 24 -- Content-Type: text/plain } 13, 24 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
-- Mr. Ron Doole Department of Materials Senior Instrumentation Engineer. University of Oxford. Phone +44 (0) 1865 273701 Parks Road. Oxford. OX1 3PH Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
==============================Original Headers============================== 7, 27 -- From ron.doole-at-materials.ox.ac.uk Tue Jan 17 12:26:00 2006 7, 27 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk [163.1.2.169]) 7, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HIPxqX022315 7, 27 -- for {microscopy-at-msa.microscopy.com} ; Tue, 17 Jan 2006 12:25:59 -0600 7, 27 -- Received: from smtp1.herald.ox.ac.uk ([163.1.0.247]) 7, 27 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.54) 7, 27 -- id 1EyvWo-0007nk-WD 7, 27 -- for microscopy-at-msa.microscopy.com; Tue, 17 Jan 2006 18:25:59 +0000 7, 27 -- Received: from webmail222.herald.ox.ac.uk ([163.1.0.222]) 7, 27 -- by smtp1.herald.ox.ac.uk with esmtp (Exim 3.36 #1) 7, 27 -- id 1EyvWo-0004Bf-01 7, 27 -- for microscopy-at-msa.microscopy.com; Tue, 17 Jan 2006 18:25:58 +0000 7, 27 -- Received: by webmail222.herald.ox.ac.uk (Postfix, from userid 101) 7, 27 -- id AD78A2A0B8; Tue, 17 Jan 2006 18:25:58 +0000 (GMT) 7, 27 -- Content-Type: text/plain 7, 27 -- Content-Disposition: inline 7, 27 -- Content-Transfer-Encoding: binary 7, 27 -- MIME-Version: 1.0 7, 27 -- X-Mailer: MIME-tools 5.411 (Entity 5.404) 7, 27 -- From: Ron Doole {ron.doole-at-materials.ox.ac.uk} 7, 27 -- Date: Tue, 17 Jan 2006 18:25:58 +0000 (GMT) 7, 27 -- In-Reply-To: {200601171752.k0HHqH72018071-at-ns.microscopy.com} 7, 27 -- To: microscopy-at-msa.microscopy.com 7, 27 -- Subject: Re: [Microscopy] TEM - Electron Beam Clearing 7, 27 -- X-Webmail-Originating-Ip: 192.76.27.49 7, 27 -- X-Webmail-Sender: rdoole 7, 27 -- Message-Id: {20060117182558.AD78A2A0B8-at-webmail222.herald.ox.ac.uk} ==============================End of - Headers==============================
Gary; Hitting ion pumps with a hammer also gets them started. The hammer is also very good at knocking loose whiskers that form in the ion pump.
John Mardinly Intel
The opinion of this author does not necessarily represent the opinion of Intel Corporation.
-----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: Monday, January 16, 2006 1:41 PM To: Mardinly, John
I too am not aware of any water cooling for ion pumps. when first pumping, they do get warm. But their controller will shut them off for a cool down.
The main problem I have had in the past is getting them to start pumping. Initially, no pump current. This is fixed by heating the pump with a hair dryer. Then they fire and that is that. This is typical of the little 1-5L/m gun chamber pumps for LaB6. The larger 30L/m pumps don't seem to have this problem. This was with Varian pumps.
gary g.
At 12:53 PM 1/16/2006, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Mon Jan 16 15:40:09 2006 11, 20 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k0GLe9od004433 11, 20 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jan 2006 15:40:09 -0600 11, 20 -- Received: (qmail 5325 invoked from network); 16 Jan 2006 13:40:08 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 5322, pid: 5323, t: 0.1787s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 20 -- by qsmtp2 with SMTP; 16 Jan 2006 13:40:08 -0800 11, 20 -- Message-Id: {6.2.3.4.2.20060116133653.0206f088-at-mail.calweb.com} 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 11, 20 -- Date: Mon, 16 Jan 2006 13:40:08 -0800 11, 20 -- To: bigelow-at-engin.umich.edu 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] RE: Starting Ion Pumps without cooling water 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200601162053.k0GKrXr6030172-at-ns.microscopy.com} 11, 20 -- References: {200601162053.k0GKrXr6030172-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
==============================Original Headers============================== 20, 34 -- From john.mardinly-at-intel.com Tue Jan 17 15:16:13 2006 20, 34 -- Received: from scsfmr003.sc.intel.com (fmr23.intel.com [143.183.121.15]) 20, 34 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HLGCFX009840 20, 34 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 17 Jan 2006 15:16:13 -0600 20, 34 -- Received: from scsfmr101.sc.intel.com (scsfmr101.sc.intel.com [10.3.253.10]) 20, 34 -- by scsfmr003.sc.intel.com (8.12.10/8.12.10/d: major-outer.mc,v 1.1 2004/09/17 17:50:56 root Exp $) with ESMTP id k0HLGCK7006008; 20, 34 -- Tue, 17 Jan 2006 21:16:12 GMT 20, 34 -- Received: from scsmsxvs041.sc.intel.com (scsmsxvs041.sc.intel.com [10.3.90.10]) 20, 34 -- by scsfmr101.sc.intel.com (8.12.10/8.12.10/d: major-inner.mc,v 1.2 2004/09/17 18:05:01 root Exp $) with SMTP id k0HLC3jR001619; 20, 34 -- Tue, 17 Jan 2006 21:12:06 GMT 20, 34 -- Received: from scsmsx331.amr.corp.intel.com ([10.3.90.4]) 20, 34 -- by scsmsxvs041.sc.intel.com (SAVSMTP 3.1.7.47) with SMTP id M2006011713160707553 20, 34 -- ; Tue, 17 Jan 2006 13:16:07 -0800 20, 34 -- Received: from scsmsx403.amr.corp.intel.com ([10.3.90.18]) by scsmsx331.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.211); 20, 34 -- Tue, 17 Jan 2006 13:16:07 -0800 20, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 20, 34 -- Content-class: urn:content-classes:message 20, 34 -- MIME-Version: 1.0 20, 34 -- Content-Type: text/plain; 20, 34 -- charset="us-ascii" 20, 34 -- Subject: RE: [Microscopy] Starting Ion Pumps without cooling water 20, 34 -- Date: Tue, 17 Jan 2006 13:16:03 -0800 20, 34 -- Message-ID: {9E1ED6A623D8CB44B4866ACF20ECDB2B08CC4267-at-scsmsx403.amr.corp.intel.com} 20, 34 -- X-MS-Has-Attach: 20, 34 -- X-MS-TNEF-Correlator: 20, 34 -- Thread-Topic: [Microscopy] Starting Ion Pumps without cooling water 20, 34 -- Thread-Index: AcYa5dJSmizC6M7cSkqL66kBhLViXgAxSKoQ 20, 34 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 20, 34 -- To: {gary-at-gaugler.com} 20, 34 -- Cc: {Microscopy-at-MSA.Microscopy.Com} 20, 34 -- X-OriginalArrivalTime: 17 Jan 2006 21:16:07.0634 (UTC) FILETIME=[3B630320:01C61BAB] 20, 34 -- X-Scanned-By: MIMEDefang 2.52 on 10.3.253.10 20, 34 -- Content-Transfer-Encoding: 8bit 20, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0HLGCFX009840 ==============================End of - Headers==============================
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Several glass plates ~5 mm thick and 15 cm square. Polishing clothes stuck to the glass plates. Glass plates inside large tray. Water spray to lubricate. Cost is almost nothing apart from the time. Depends how large the sample is and what finish you are working from. Fine grinding can be done by using aumina directly onto the glass. It's the way I used to prepare 3 mm steel discs for TEM, before a final electropolish. A 3 mm stainless steel disc takes ~15 mins to polish by hand from the alumina grinding.
-- Larry Stoter
PLEASE NOTE 1. Any mail other than plain text will be automatically deleted. 2. Any mail, legitimate or not, apparently or actually from hotmail, netscape, yahoo or excite will automatically be deleted. 3. Mail with no subject or without a clear subject will be ignored :-)
==============================Original Headers============================== 4, 16 -- From larry-at-cymru.freewire.co.uk Tue Jan 17 15:45:45 2006 4, 16 -- Received: from get.freewire.net (get.freewire.net [195.184.229.250]) 4, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HLjhPu015336 4, 16 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 17 Jan 2006 15:45:44 -0600 4, 16 -- Received: from [217.154.248.196] (th1dc-217-154-248-196.dial.mistral.co.uk [217.154.248.196]) 4, 16 -- by get.freewire.net (8.11.6/8.11.6) with ESMTP id k0HLjdC13695; 4, 16 -- Tue, 17 Jan 2006 21:45:40 GMT 4, 16 -- Mime-Version: 1.0 4, 16 -- Message-Id: {p06210200bff31079bb79-at-[217.154.248.193]} 4, 16 -- In-Reply-To: {200601161546.k0GFkBfX020754-at-ns.microscopy.com} 4, 16 -- References: {200601161546.k0GFkBfX020754-at-ns.microscopy.com} 4, 16 -- Date: Tue, 17 Jan 2006 21:33:35 +0000 4, 16 -- To: lemaster-at-ppg.com, Microscopy-at-MSA.Microscopy.Com 4, 16 -- From: Larry Stoter {larry-at-cymru.freewire.co.uk} 4, 16 -- Subject: Re: [Microscopy] SEM advice on low cost polisher 4, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Oh yeah. I forgot to mention that trick too. I usually use the handle end of a screwdriver. This also works for cold cathode sensors too.
gary g.
At 01:33 PM 1/17/2006, you wrote:
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==============================Original Headers============================== 8, 20 -- From gary-at-gaugler.com Tue Jan 17 17:13:09 2006 8, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 8, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k0HND8OF000462 8, 20 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2006 17:13:08 -0600 8, 20 -- Received: (qmail 2929 invoked from network); 17 Jan 2006 15:13:06 -0800 8, 20 -- Received: by simscan 1.1.0 ppid: 2926, pid: 2927, t: 0.2584s 8, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 8, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 8, 20 -- by qsmtp1 with SMTP; 17 Jan 2006 15:13:06 -0800 8, 20 -- Message-Id: {6.2.3.4.2.20060117151146.01febf50-at-mail.calweb.com} 8, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 8, 20 -- Date: Tue, 17 Jan 2006 15:13:07 -0800 8, 20 -- To: john.mardinly-at-intel.com 8, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 8, 20 -- Subject: Re: [Microscopy] RE: Starting Ion Pumps without cooling water 8, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 8, 20 -- In-Reply-To: {200601172133.k0HLXwjI013245-at-ns.microscopy.com} 8, 20 -- References: {200601172133.k0HLXwjI013245-at-ns.microscopy.com} 8, 20 -- Mime-Version: 1.0 8, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
With some degree of simplification- low signal electronic imaging is a race between noise accumulation and signal accumulation.
Some of the noise sources in the CCD are:
a) thermal noise (reduced by factor of 2 for every 6 to 7 degrees C temperature drop for silicone CCD), mostly correctable by dark frame subtraction; b) readout (pixel clock switching) noise also know as bias noise, somewhat reduced by cooling, correctable by bias frame subtraction; c) random (shot) noise - a random part of thermal noise (a) which can not be corrected by dark frame subtraction- this noise gets worse with pixels size shrinking (the larger the pixels the less random this value is)- a crude comparison is a mechanical vibration dumping by increasing weight (anti-vibration platform).
Assuming your signal source is set (optics, specimen, illumination):
a) the colder the sensor- the better S/N ratio is- noise accumulation slows down, while signal stays the same; b) with 30 deg. C difference noise will be different by factor of 32 (30deg. = 6deg.*5; noise drops twice per every 6 degrees, thus 2 power 5 = 32 which is a dramatic noise reduction, but noticeable only if light is dim and exposures are long. c) the larger the pixels- the better S/N ratio is- at the expense of spatial resolution- it could pay to have more smaller pixels and use binning instead when needed.
Type of sensor:
a) definitely CCD over CMOS; b) probably full frame CCD over interline CCD, (full frame has much better S/N ratio for a given pixel size), but interline CCD could be more convenient due to potentially faster frame rate, exposure time allowing; c) if exposure time must be long, interline CCD looses it's potential speed advantage, and it is always noisier than full frame one; d) interline CCD has typically 50% of it's light receiving area occupied by transfer gates, meaning that only half of it is light sensitive- this can be improved by micro lens array - most interline CCDs are available in such configuration), but sensitive area of the pixel is still half of what it could be in full frame CCD with same pixel size, which means higher noise .
Interline CCDs can use frame integration with less or no cooling and achieve decent noise reduction, but this is always inferior to deeply cooled full frame CCD in single frame (long exposure) integration mode, with respect to S/N.
I deliberately used S/N (signal to noise) ratio instead just "noise", because it is precisely ratio that counts. If signal is strong (bright light), then it accumulates much faster than noise. Then exposures are short, and no CCD cooling needed. There is no straight answer without knowing the numbers for a given application.
A specific application could be best served by either a CMOS, or a full frame CCD, or interline CCD sensor, cooled or not- many more variables must be taken into account.
Vitaly Feingold SIA 2773 Heath Line Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com
----- Original Message ----- X-from: {phillipst-at-missouri.edu} To: {vitalylazar-at-att.net} Sent: Friday, January 13, 2006 12:54 PM
Overview: Schafer Vallecitos Laboratory is seeking a Senior Engineer /Scientist to add to our mass spectrometry group. SVL performs materials characterization and related analytical services on commercial and government contracts. The activities of the group include chemical and elemental analysis of materials on a production basis, maintenance of several mass spectrometers and ancillary equipment, development of new or improved MS analysis techniques, and quality assurance of analytical data. Schafer is a technically strong firm with a reputation for quality and integrity. Schafer's reputation is a direct result of our dedicated, motivated, talented, and creative staff that is responsible for developing our outstanding business relationships with our customers. Our technical capabilities are vast and growing to provide innovations for the future.
Responsibilities: This position requires an individual who has proven technical abilities to function as an instrument designer, with emphasis on ion optics, electronics, and computer automation. Commercial design experience would be a plus, as the instrument should be maintainable and replicable, not a one-of-a-kind that requires a large staff to maintain. The ideal candidate must have a strong background in materials science or engineering, or a related field of physics, geology, chemistry, or metrology. Experience in design and operation of mass spectrometers, particularly TIMS, and other analytical instruments, is highly recommended. The candidate should have competence in vacuum technology, electronics, mechanical design, ion optics, and project administration.
Qualifications: Experience with analytical instrument control computer hardware and software is required. The candidate must be able to operate independently with occasional supervision.
Other qualifications include:
. Bachelor's degree in physical science or engineering. . Minimum 10 years of technical experience. . Demonstrated ability to solve technical problems. . Expertise in data evaluation and quality control. . Proven ability to write clear scientific reports and proposals. . Must be a US citizen with the ability to obtain government security clearance.
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There are two systems I know of that he may have overlooked:
One is the (dot)Scan, by Soft Imaging Systems The second is the Mirax system, by Carl Zeiss
I know they can physcially do what you require (overnight, automated loading and unloading), but I don't know their abilities in terms of stains.
Hope that helps a little bit! -Chris
--------------------------------- Christopher Hayden SPA Novartis Pharmaceuticals
Hi
Can anyone out there help a colleague?
"I am discussing proposals to install an imaging=20 system here. It is one that can capture both FISH=20 and brown/ H&E stains and then allow an operator,=20 using selected software, to analyse them. The=20 favoured system at present is the Applied Imaging=20 one as it is now undergoing a major facelift.
I am just wondering if there are any other=20 systems on the market right now that can load=20 up 50 slides automatically overnight, scan &=20 store so that an operator can then analyse the=20 images captured the following morning. None of=20 the other major suppliers here in the UK, such as=20 ChromaVision, Meta-systems & Aperio can offer=20 both overnight loading and scanning of both FISH=20 and brown/ blue & pink stains at present."
Dear Yossi, I have not seen a reply to your inquiry, so I will tell you what I know, which isn't much. Of all of the mechanisms for contrast in the back-scattered-electron (BSE) detector, the magnetic domain is the lowest. First is atomic number, second is channeling contrast and third is magnetic domain. This means you must eliminate all others to see the magnetic domain. The sample must be single phase, preferably single crystal and flat, parallel to the BSE detector, well-polished and, perhaps, electropolished. I have not done this, only seen talks about it. It takes a lot of beam current, an optimum working distance for the BSE (about 20 to 15 mm), all quadrants on and contrast on the BSE amplifier as high as practical. And a lot of patience. Good luck. Regards, Mary Mager Electron Microscopist Department of Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA Tel: 604-822-5648 Fax: 604-822-3619 e-mail: mager-at-interchange.ubc.ca ----- Original Message ----- X-from: {yezer-at-cc.hut.fi} To: {mager-at-interchange.ubc.ca} Sent: Tuesday, January 17, 2006 1:01 AM
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Email: bucana-at-audumla.mdacc.tmc.edu Name: Corazon D. Bucana
Organization: UT MD Anderson Cancer Center
Title-Subject: [Filtered] Separation of resin from glass tissue culture chamber slides
Question: We have emdedded tissue culture cells grown on tissue culture glass chambered slides using Polybed Epon 812, polymerized at 60C for 2 days. We tried to separate the glass by using liquid nitrogen followed by warm water bath but the glass did not separate from the polymerized resin. Can anyone suggest an alternative procedure to remove the glass from the block? Thanks.
We grow the cells on a coverslip (round coverslips are better and they will fit into the chamber) and remove them by hydrofluoric acid afterwards. Works very well, although the HF is not very pleasant to work with.
Good luck,
M.
-- Michael Jarnik, Ph.D. Electron Microscope Facility Fox Chase Cancer Center 7701 Burholme Ave. Philadelphia, PA 19111 Tel. 215-728-5675 Fax 215-728-2412
Name: Corazon D. Bucana
Organization: UT MD Anderson Cancer Center
Title-Subject: Separation of resin from glass tissue culture chamber slides
Question: We have emdedded tissue culture cells grown on tissue culture glass chambered slides using Polybed Epon 812, polymerized at 60C for 2 days. We tried to separate the glass by using liquid nitrogen followed by warm water bath but the glass did not separate from the polymerized resin. Can anyone suggest an alternative procedure to remove the glass from the block? Thanks.
==============================Original Headers============================== 13, 19 -- From M_Jarnik-at-fccc.edu Thu Jan 19 19:23:54 2006 13, 19 -- Received: from azah.fccc.edu (azah.fccc.edu [131.249.4.237]) 13, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0K1Nr1r023799 13, 19 -- for {microscopy-at-microscopy.com} ; Thu, 19 Jan 2006 19:23:53 -0600 13, 19 -- Received: from fccc.edu (emf4.fccc.edu [131.249.9.170]) 13, 19 -- by azah.fccc.edu (8.12.11/8.12.11) with ESMTP id k0K1Nrxr008126 13, 19 -- for {microscopy-at-microscopy.com} ; Thu, 19 Jan 2006 20:23:53 -0500 (EST) 13, 19 -- Message-ID: {43D03BA9.5060405-at-fccc.edu} 13, 19 -- Date: Thu, 19 Jan 2006 20:23:53 -0500 13, 19 -- From: Michal Jarnik {M_Jarnik-at-fccc.edu} 13, 19 -- Reply-To: M_Jarnik-at-fccc.edu 13, 19 -- Organization: Fox Chase Cancer Center 13, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 13, 19 -- X-Accept-Language: en,cs 13, 19 -- MIME-Version: 1.0 13, 19 -- To: microscopy-at-microscopy.com 13, 19 -- Subject: RE: Separation of resin from glass tissue culture chamber slides 13, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 13, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi. We too use concentrated hydrofluoric acid to dissolve glass coverslips. If you use plastic tripour beakers, plastic forceps, and plenty of rinse water, it doesn't cause problems. Since slides are thicker than coverslips, they may need may need longer time in the acid. Coverslips usually dissolve in 20 mins if all the edges are free of resin. To help the acid get at the free edges, file down the edges of the embedded slides using a metal file. Then immerse in acid under the fume hood in a plastic beaker. Check periodically by dipping in water and looking at the surface( wear nitrile gloves and work under the hood) The surface should be smooth and shiny with no patches of undissolved glass( looks like an iceberg melting). Rinse well under running water and dry in the embedding oven. Good luck!
JoAnn Buchanan Stanford University school of Medicine Stanford, CA 94305
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Title-Subject: [Filtered] Immunocytochemistry for TEM
Question: Greetings ListServers,
Does anyone have a general protocol for labelling anti-lucifer yellow using protein-A gold as a bridging antibody in the Locust (Orthopteran) brain? Should I try to immunogold pre-embedding, using a detergent such as Saponin? Should I dissect out the brain before fixation? I am hoping to use the immunogold labelling to help me visualize neuronal connections. Any advice is greatly appreciated.
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Title-Subject: [Filtered] liposomes analyzed by Freeze fracture SEM
Question: Hello,
I work for a company that is interested in getting some liposomes analyzed by Freeze fracture SEM or another SEM type technique. If there is any way you could put me into contact with some of your members who do this type of work or to post my request on a list serve, etc. it would be extremely helpful.
Thank you,
Michael Beverly Sirna Therapeutics, Inc. 303-546-8190
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Title-Subject: [Filtered] NANOBIO summer school in Cargese France
Question: Please find enclosed a flyer on the NANOBIO summer school in Cargese, Corsica, France from July 17th till 29th, 2006.
Watching single biological molecules at work is now possible and the unravelled features of the molecular machinery at the nanoscale are currently changing our view of the cell. Though, bridging the gap between nano- and micro-world is not easy: how to relate single molecule events to biological functions at the cell level? how do molecular structures assemble, coordinate and integrate in the wholeÖ? Thanks to physicists and biologists, we will address these questions and try to give some answers. Speakers will present recent concepts and methods in physics and biology dedicated to single molecule observation and description of the cell at the sub-micron level. Particular emphasis will be given to the optical methods, which allow the observation of live cells in physiological conditions.
This school is part of the Nanosciencestech Marie Curie Series of Events: http://www.france-optique.org/Nanosciencestech.html
The students, including PhDs, post-docs, and young researchers, will be encouraged to present their own work (also in adjacent fields) during poster sessions.
A European and more generally an international audience is targeted, and all lectures in the summer school will be given in English.
Important dates: Pre-registration: Application deadline 28 february 2006 Selected participant will be informed before the end of march
Registration and paying attendees deadline: 30 April 2006
All correspondence should be sent to
SociÈtÈ FranÁaise d'Optique Nanobio summer school c/o FranÁoise CHAVEL Centre Scientifique d'Orsay - B’t. 503 91403 ORSAY cedex France
next time, you should only polymerize for about 7-8 hours before popping the cells off. I then usually re-embed the pieces that come off and polymerize using standard times.
At 06:43 PM 01/19/06, you wrote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
Greetings ListServers, does anybody have any experience using TUNEL assay on plants? I use the kit from Roche, nucleotides dyed with TMR, and have a problem that all the seccions (free-hand,fixed,penetrated with Triton and pectinase/cellulase solution), even those treated just with labeling solution without enzymes, are TUNEL positive. Do you know any reasonable explanation how could the nucleotides bind on the nucleus (and slightly on plasmatic membrane) without enzymes added?
Best regards,
Zuzana Lenochova Charles University Prague
==============================Original Headers============================== 7, 21 -- From lenoska1-at-seznam.cz Fri Jan 20 09:10:21 2006 7, 21 -- Received: from mx1.seznam.cz (mx1.seznam.cz [212.80.76.26]) 7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k0KFAIIa017250 7, 21 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Jan 2006 09:10:19 -0600 7, 21 -- Received: (qmail 21368 invoked by uid 0); 20 Jan 2006 15:10:18 -0000 7, 21 -- To: Microscopy-at-Microscopy.Com 7, 21 -- Date: Fri, 20 Jan 2006 16:10:16 +0100 (CET) 7, 21 -- Reply-To: =?iso-8859-2?Q?Zuzana=20Lenochov=E1?= {lenoska1-at-seznam.cz} 7, 21 -- From: =?iso-8859-2?Q?Zuzana=20Lenochov=E1?= {lenoska1-at-seznam.cz} 7, 21 -- Received: from [195.113.47.73] 7, 21 -- by email.seznam.cz with HTTP 7, 21 -- for lenoska1-at-seznam.cz; 7, 21 -- Fri, 20 Jan 2006 16:02:18 +0100 (CET) 7, 21 -- Subject: =?us-ascii?Q?fluorescence=20microscopy?= 7, 21 -- Mime-Version: 1.0 7, 21 -- Message-Id: {4290.7421-27152-1432019944-1137769816-at-seznam.cz} 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- Content-Type: text/plain; 7, 21 -- charset="us-ascii" 7, 21 -- X-Abuse: helpdesk-at-seznam.cz 7, 21 -- X-Seznam-User: lenoska1-at-seznam.cz ==============================End of - Headers==============================
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I've never done this and I'm trying to remember an illustration from an old JEOL SEM brochure, which described an attachment for magnetic domain imaging.
From what I recall, the experimental setup was similar to that used now for EBSD. Sample at a high tilt with a detector to one side. In this case, a BSE detector. The idea was that the weak effect of the magnetic domains caused a small divergence of BS electrons. By positioning the detector a long way from the sample and at a high angle from the incident beam, the divergence was amplified. -- Larry Stoter
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Even though I am very experienced in Electron Microscopy, there are times when I cut LM sections that are extremely wrinkled, and they look like cracked glass. I normally don't experience this, but when it happens, even considering every possible variable, I'm still not exactly sure what might be at work here to give this sort of result.
I'm looking for ideas here as to what might be causing this effect. What do people think?
Garry Burgess Charge Technologist - Electron Microscopy Health Sciences Centre Winnipeg, Canada.
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==============================Original Headers============================== 6, 20 -- From GBurgess-at-exchange.hsc.mb.ca Fri Jan 20 16:10:02 2006 6, 20 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca [142.233.100.122]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0KMA1mn017769 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Jan 2006 16:10:01 -0600 6, 20 -- Received: from hscxntmx0007.hsc.mb.ca (unverified [172.16.6.179]) by 6, 20 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS 4.0.346.0) with ESMTP id 6, 20 -- {B0017584293-at-hscxntmx0003.hsc.mb.ca} for {Microscopy-at-microscopy.com} ;Fri, 6, 20 -- 20 Jan 2006 16:09:53 -0600 6, 20 -- Received: by hscxntmx0007.hsc.mb.ca with Internet Mail Service 6, 20 -- (5.5.2653.19)id {T7CLC1WX} ; Fri, 20 Jan 2006 16:10:27 -0600 6, 20 -- Message-ID: 6, 20 -- {00A937989100304A83A058F6C45873FF32A36C-at-hscxntmx0005.hsc.mb.ca} 6, 20 -- Date: Fri, 20 Jan 2006 16:06:48 -0600 6, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 6, 20 -- Subject: Wrinkled LM Sections 6, 20 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 6, 20 -- X-Mailer: Internet Mail Service (5.5.2653.19) 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="iso-8859-1" ==============================End of - Headers==============================
in my experience (I am familiar with large semithin sections = up to 5 x 5 mm from human diagnostic samples for some 20 years now) your question cannot be answered with one sentence. There are several parameters to be included in a thoroughly checking of causes, starting from the type of tissue, via fixation, (full) dehydration, intermedium (full evaporation/complete exchange by resin), type, quality [polymerisation, hardness] of resin, quality of knife edge, sectioning parameters (knife, cutting angle, speed) and type of transfer of sections from the water trough to the slide, and last but not least, "special treatment" of the floating section on a water drop (i.e., for example, trying to spread sections by xylene vapor [other - more healthier - vapors?] or gentle - longer - warming up by means of a light bulb positioned above the section/slide). So IMO, you have to discriminate the problem(s) from the whole processing schedule.......(;-(.....
You tell us that you face problems not everytime, but sometimes..... Could you give us some examples (tissue type, some hints on chemicals /resin you use?) where you get such results? (not to forget the dimensions of the tissue blocks and type of knife used)....
Perhaps there is a simple solution......who knows,
best regards and have a nice weekend,
Wolfgang Muss
Salzburg, Austria
Paracelsus Medical Private University (PMU) Institute of Pathology Electron Microscopy Lab Muellner Hauptstrasse 48 A-5020 SALZBURG, Austria/Europe Phone work: +43+662+4482+4720 Mobile phone work:+43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss") E-Mail work: W.Muss-at-SALK.at
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I'm looking for ideas here as to what might be causing this effect. What do people think?
Garry Burgess Charge Technologist - Electron Microscopy Health Sciences Centre Winnipeg, Canada.
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==============================Original Headers============================== 6, 20 -- From GBurgess-at-exchange.hsc.mb.ca Fri Jan 20 16:10:02 2006 6, 20 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca [142.233.100.122]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0KMA1mn017769 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Jan 2006 16:10:01 -0600 6, 20 -- Received: from hscxntmx0007.hsc.mb.ca (unverified [172.16.6.179]) by 6, 20 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS 4.0.346.0) with ESMTP id 6, 20 -- {B0017584293-at-hscxntmx0003.hsc.mb.ca} for {Microscopy-at-microscopy.com} ;Fri, 6, 20 -- 20 Jan 2006 16:09:53 -0600 6, 20 -- Received: by hscxntmx0007.hsc.mb.ca with Internet Mail Service 6, 20 -- (5.5.2653.19)id {T7CLC1WX} ; Fri, 20 Jan 2006 16:10:27 -0600 6, 20 -- Message-ID: 6, 20 -- {00A937989100304A83A058F6C45873FF32A36C-at-hscxntmx0005.hsc.mb.ca} 6, 20 -- Date: Fri, 20 Jan 2006 16:06:48 -0600 6, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 6, 20 -- Subject: Wrinkled LM Sections 6, 20 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 6, 20 -- X-Mailer: Internet Mail Service (5.5.2653.19) 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="iso-8859-1" ==============================End of - Headers==============================
==============================Original Headers============================== 25, 28 -- From W.Muss-at-salk.at Sat Jan 21 04:43:58 2006 25, 28 -- Received: from Hermes.salk.at (hermes.lks.at [193.170.167.9]) 25, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0LAhv9E003479 25, 28 -- for {Microscopy-at-microscopy.com} ; Sat, 21 Jan 2006 04:43:57 -0600 25, 28 -- Received: from localhost (localhost [127.0.0.1]) 25, 28 -- by hermes.lks.at (Postfix) with ESMTP id B185A5A9046; 25, 28 -- Sat, 21 Jan 2006 11:43:51 +0100 (CET) 25, 28 -- Received: from hermes.lks.at ([127.0.0.1]) 25, 28 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024) 25, 28 -- with ESMTP id 42900-09; Sat, 21 Jan 2006 11:43:51 +0100 (CET) 25, 28 -- Received: from c1pa003 (unknown [192.168.42.3]) 25, 28 -- by hermes.lks.at (Postfix) with SMTP id 754D65A9044; 25, 28 -- Sat, 21 Jan 2006 11:43:51 +0100 (CET) 25, 28 -- Received: by localhost with Microsoft MAPI; Sat, 21 Jan 2006 11:43:49 +0100 25, 28 -- Message-ID: {01C61E7F.F216D3E0.W.Muss-at-salk.at} 25, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at} 25, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at} 25, 28 -- To: "'GBurgess-at-exchange.hsc.mb.ca'" {GBurgess-at-exchange.hsc.mb.ca} , 25, 28 -- "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 25, 28 -- Subject: [Microscopy] Re: Wrinkled LM Sections 25, 28 -- Date: Sat, 21 Jan 2006 11:43:48 +0100 25, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at} 25, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor 25, 28 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211 25, 28 -- MIME-Version: 1.0 25, 28 -- Content-Type: text/plain; charset="us-ascii" 25, 28 -- Content-Transfer-Encoding: 7bit 25, 28 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at ==============================End of - Headers==============================
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Email: wong-at-msg.ucsf.edu Name: Mei Lie Wong
Title-Subject: [Filtered] formvar
Question: When using formvar in ethylene dichloride, is the any way to make the films slightly thicker. I know when using formvar in chloroform, you can just drain slower or faster to make films thinner or thicker. Is there something like this for the ethylene dichloride formula? I have tried several different times but so far haven't hit on anything totally satisfactory.
Mei Lie Wong wrote: ======================================================== Question: When using formvar in ethylene dichloride, is the any way to make the films slightly thicker. I know when using formvar in chloroform, you can just drain slower or faster to make films thinner or thicker. Is there something like this for the ethylene dichloride formula? I have tried several different times but so far haven't hit on anything totally satisfactory. ======================================================= This is discussed on http://www.2spi.com/catalog/submat/sup_film6.html
Disclaimer: SPI Supplies manufactures Formvar coated grids for customers and we disclose some of the tricks we use for making thicker or thinner films when using ethylene dichloride.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
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==============================Original Headers============================== 10, 25 -- From cgarber-at-2spi.com Sat Jan 21 10:41:36 2006 10, 25 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 10, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0LGfaP8025961 10, 25 -- for {microscopy-at-msa.microscopy.com} ; Sat, 21 Jan 2006 10:41:36 -0600 10, 25 -- Received: from ibm1x23g2abfyg ([71.225.72.4]) 10, 25 -- by diskless5.axs2000.net (8.12.11/8.12.11) with SMTP id k0LGfYOZ012677; 10, 25 -- Sat, 21 Jan 2006 11:41:35 -0500 10, 25 -- X-IDV-FirstRcvd: [71.225.72.4] 10, 25 -- X-IDV-HELO: ibm1x23g2abfyg 10, 25 -- Message-ID: {01e401c61ea9$883ec840$6401a8c0-at-ibm1x23g2abfyg} 10, 25 -- Reply-To: "Garber, Charles A." {cgarber-at-2spi.com} 10, 25 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 10, 25 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 10, 25 -- Subject: Formvar filmed grids: controlling thickness 10, 25 -- Date: Sat, 21 Jan 2006 11:41:30 -0500 10, 25 -- MIME-Version: 1.0 10, 25 -- Content-Type: text/plain; 10, 25 -- format=flowed; 10, 25 -- charset="Windows-1252"; 10, 25 -- reply-type=original 10, 25 -- Content-Transfer-Encoding: 7bit 10, 25 -- X-Priority: 3 10, 25 -- X-MSMail-Priority: Normal 10, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 10, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
I was interested in the comments about banging on the sides of a sputter ion pump with a hammer or screw driver to overcome the startup problem that arises when a whisker or flake of titanium has formed and shorted out the path between the cathode and anode of the pump. Another way to treat this problem is discussed on p. 295 of my book 'Vacuum Methods in Electron Microscopy' (available from SPI, Ladd, M.E. Taylor, etc. For a description see: www.2spi.com/catalog/books/book48.html). This involves allowing the pressure in the pump to rise slightly above 1 Pa (10-2 Torr) and then turning on the high voltage power for a few seconds for three or four times. Be careful not to do this long enough to damage the power supply. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-engin.umich.edu; Fx:734-763-4788; Ph:734-662-5237 Address mail to: 1136 Mixtwood Rd. Ann Arbor, MI 48103-3035
==============================Original Headers============================== 1, 14 -- From bigelow-at-engin.umich.edu Sat Jan 21 11:29:43 2006 1, 14 -- Received: from srvr22.engin.umich.edu (srvr22.engin.umich.edu [141.213.75.21]) 1, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0LHTgo2002820 1, 14 -- for {microscopy-at-microscopy.com} ; Sat, 21 Jan 2006 11:29:42 -0600 1, 14 -- Received: from [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 1, 14 -- by srvr22.engin.umich.edu (8.13.5/8.13.5) with ESMTP id k0LHTfRg008116 1, 14 -- for {microscopy-at-microscopy.com} ; Sat, 21 Jan 2006 12:29:42 -0500 (EST) 1, 14 -- Mime-Version: 1.0 1, 14 -- Message-Id: {p06210202bff81ae0e465-at-[141.212.131.221]} 1, 14 -- Date: Sat, 21 Jan 2006 12:29:41 -0500 1, 14 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 1, 14 -- From: Wil Bigelow {bigelow-at-engin.umich.edu} 1, 14 -- Subject: [Microscopy] RE: Starting Ion Pumps 1, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Yes, that might also work. However, if I just got through with an 8 hour bakeout, why would I want to degrade vacuum in the gun chamber? I would either never get the chamber pumped down or it would take a very long time to reach terminal vacuum.
The idea is to get vacuum as good as possible and then isolate the gun chamber before turning on the pump. Even so, startup current would be high.
I've used the hair dryer most of the time. Then, sometimes whack the pump with the screwdriver handle. This is only for the small pumps. For some reason, they don't start as readily as the larger ones.
gary g.
At 09:48 AM 1/21/2006, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Sat Jan 21 12:09:22 2006 10, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k0LI9KuQ010814 10, 20 -- for {microscopy-at-microscopy.com} ; Sat, 21 Jan 2006 12:09:21 -0600 10, 20 -- Received: (qmail 22469 invoked from network); 21 Jan 2006 10:09:20 -0800 10, 20 -- Received: by simscan 1.1.0 ppid: 22466, pid: 22467, t: 0.1656s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp1 with SMTP; 21 Jan 2006 10:09:20 -0800 10, 20 -- Message-Id: {6.2.3.4.2.20060121100351.020356f8-at-mail.calweb.com} 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 10, 20 -- Date: Sat, 21 Jan 2006 10:09:22 -0800 10, 20 -- To: bigelow-at-engin.umich.edu 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] RE: Starting Ion Pumps 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200601211748.k0LHmBJV006678-at-ns.microscopy.com} 10, 20 -- References: {200601211748.k0LHmBJV006678-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Celebration of life for Delbert E. Philpott September 24, 1923 - December 11, 2005
Yesterday at the Liberty Lodge Masonic Center in Santa Clara, CA, we honored the life and friendship of Dr. Del Philpott, a lifelong microscopist and soldier who truly believed in the perpetuation of peace and worked for that goal his entire life.
His wife, Donna, agreed to our reprinting of a few of his many achievements which she listed in the memorial pamphlet.
Del was born and raised in Omro, Wisconsin. Dr. Philpott was a World War II Veteran who served with the Fighting 69th Infantry Division as they raced across Europe to link up with the Russian 58th Guards at the Elbe River in Germany in April of 1945. The famous link-up photo, by photographer Allan Jackson of 3 Americans (including Del), and 3 Russians shaking hands was printed in newspapers across the United States. The soldiers however didn't see the photo because the war hadn't yet ended. Fellow Infantryman Sam Popkins, who had helped Allan recruit soldiers for the photo, later told Del about it and confirmed that Del was one the soldiers in the photo. Del was awarded the Bronze Star and Purple Heart Medals for his service.
After the war, Del completed his Bachelor's Degree in chemistry at Indiana University in 1948 and his Master's Degree in 1949. As a Research Associate at the University of Illinois Medical School in Chicago from 1949 to 1952, he established the first electron microscope facility for the medical school. He was Head of Electron Microscopy, Marine Biological Lab and Institute for Muscle Research, Woods Hole, MA from 1952 to 1963, where he established the electron microscope facility for the 2 Institutes.
A pioneer in the field of Electron Microscopy, he directed and ran research projects under Nobel Laureate Dr. Albert Szent-Gyorgyi in the winter and for the Marine Biological Lab at Woods Hole during the summer. He built his own ultramicrotome for ultra-thin sectioning.
He published the first paper on ultrastructure of the human heart with Dr. Bruno Kish and published the first electron micrographs of a protein crystal isolated from muscle. He demonstrated that ribosomes travel from the nucleus to the cytoplasm via the nuclear pores. Del got his PhD in cytology in 1963 at Boston University.
While Professor of Biochemistry at the University of Colorado from 1963 to 1965, he established and ran the electron microscope laboratory at the medical school. In 1966, he was Head of the Department of Electron Microscopy and Co-Director of the Institute for Biomedical Research at Mercy Hospital in Denver, CO.
Del came to California in the 60's to work as a Research Scientist as the Head of the Ultrastructure Laboratory at the Moffett Field NASA Ames Research Center. He inspected lunar soil for signs of life and had experiments flown on both American and Russian spacecraft including Apollo 17, Cosmos 736, Cosmos 936, and SL-3. He developed a fixative for space flight that preserves the ultrastructure of tissues for over 4 years without the need for embedding. Del retired from NASA in 1990, but worked under contract with the Lockheed Martin Company at NASA Ames as a Research Associate whose duties included assisting visiting Russian scientists with space related experiments.
During his professional career, Del authored over 230 scientific papers and wrote articles for several books and magazines.
In 1995, Del and his wife Donna co-edited the book "Hands Across The Elbe", stories by American and Russian veterans about the link up that cut Fascist Germany in half in April 1945.
Del was one of 10 veterans chosen to be guests at the Russian government's May 9, 2005, 60th Anniversary Celebration of the end of World War II. He was selected to be seated at the table with the President of China, President Putin, and President and Mrs. Bush at the Kremlin Reception following the Parade e in Red Square.
Del's interests included flying, raising dogs, ham radio, ceramics, spinning, photography, woodcarving, magic, and travel. He held offices in many organizations including several scientific societies, veterans' organizations, and The Unique Boutique at the Sunnyvale Senior Center. He was a member of Liberty Lodge $299, Valley Star Chapter #250 O.E.S., and the San Jose Scottish Rite.
He wanted to be remembered for his part in developing interaction between the American and Russian scientists in space research. He believed that interactions among the citizens of all nations are necessary for the perpetuation of peace and felt that the "Spirit of the Elbe" encourages this vision.
Thank you Donna for putting together such an information-packed bibliography.
I knew Del from the late 60's from the microscopy society. He was a wonderful friend and had an uncanny sense of humor. I would like to share 2 of Del's favorite stories. And for those of you who knew Del, you know he had LOTS of stories.
I can no longer remember the exact year, but it was in the 70's. Someone sent in a bogus abstract for the EMSA meeting with a crazy title and crazy names of authors. Unfortunately I no longer remember those either. However the following year, as you can imagine, EMSA was trying to review the abstracts or at least look more carefully at the titles and authors. That year Del sent in an abstract about research on lunar samples. Well, whoever reviewed his abstract decided that no one could possibly be named Delbert Philpott and certainly no one was looking at moon samples, SO, his paper was rejected!!
Del's sense of humor was ever present. Before he knew Donna, again in the 70's sometime, Del was at the EMSA meeting telling us about a Valentine's present he got for his girlfriend. He bought some dog biscuits (yes dog biscuits), had them chocolate coated, put them in those fancy white wrappers, and put them in a standard Valentine's Candy Box. He gave them to his girlfriend. The next year I saw Del, I asked him how his girlfriend liked her Valentine's gift. He said "Well, I actually haven't seen her since then. I guess she didn't like that brand dog biscuits"!!!!
Del is gone, but his loving sense of humor, and desire for the promotion of peace through interactions among citizens of all nations will always be with us. We love you Del, and will miss you always.
God Bless Us All, Judy Murphy
==============================Original Headers============================== 21, 17 -- From murphyjudy-at-comcast.net Sun Jan 22 17:23:21 2006 21, 17 -- Received: from sccrmhc11.comcast.net (sccrmhc11.comcast.net [63.240.77.81]) 21, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0MNNK1W018752 21, 17 -- for {Microscopy-at-microscopy.com} ; Sun, 22 Jan 2006 17:23:21 -0600 21, 17 -- Received: from [192.168.1.102] (c-67-181-78-13.hsd1.ca.comcast.net[67.181.78.13]) 21, 17 -- by comcast.net (sccrmhc11) with ESMTP 21, 17 -- id {2006012223231901100qjb1ie} ; Sun, 22 Jan 2006 23:23:19 +0000 21, 17 -- Message-ID: {43D413E6.6010102-at-comcast.net} 21, 17 -- Date: Sun, 22 Jan 2006 15:23:18 -0800 21, 17 -- From: Judy Murphy {murphyjudy-at-comcast.net} 21, 17 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 21, 17 -- X-Accept-Language: en-us, en 21, 17 -- MIME-Version: 1.0 21, 17 -- To: Microscopy {Microscopy-at-microscopy.com} 21, 17 -- Subject: We Remember Del Philpott 21, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 21, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Gary, I have been working with LR white and have been facing similar problems. They are more so only when I immuno label those sections. Then again my sections are more than 2 mm in size and they are thin sections. I have managed to reduce the wrinkles by changing the washing techniques between immunolabelling steps but that hasnt completely eliminated the wrinkles. I have observed that LR white section around 1mm wide didnt have so many wrinkles. Unfortunately for me I cant have thicker sections or smaller sections to avoid this problem. I cannot use a different resin since it interfers with my immunolabeling. SO the bottom line being, I have learnt to live with it unfortunately. But I have a feeling that smaller sections do better and the wrinsing the grids in a drop of water/ buffer on a petridish does help reduce the wrinkles greatly if not eliminate it.
Any advice on photoshop micracles of ironing out the wrinkles would be appreciated. I hope facelifting TEM pics will not be frowned upon by the microscopy community.
Looking forward for comments and suggestion.
Regards, Vinod Nair Graduate Student Dept. of Biology New Mexico State University
On 1/21/06, W.Muss-at-salk.at {W.Muss-at-salk.at} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Good morning, all, } } Dear Garry, } } in my experience (I am familiar with large semithin sections = up to 5 x 5 } mm from human diagnostic samples for some 20 years now) your question } cannot be answered with one sentence. } There are several parameters to be included in a thoroughly checking of } causes, starting from the type of tissue, via fixation, (full) dehydration, } intermedium (full evaporation/complete exchange by resin), type, quality } [polymerisation, hardness] of resin, quality of knife edge, sectioning } parameters (knife, cutting angle, speed) and type of transfer of sections } from the water trough to the slide, and last but not least, "special } treatment" of the floating section on a water drop (i.e., for example, } trying to spread sections by xylene vapor [other - more healthier - } vapors?] or gentle - longer - warming up by means of a light bulb } positioned above the section/slide). } So IMO, you have to discriminate the problem(s) from the whole processing } schedule.......(;-(..... } } You tell us that you face problems not everytime, but sometimes..... } Could you give us some examples (tissue type, some hints on chemicals } /resin you use?) where you get such results? (not to forget the dimensions } of the tissue blocks and type of knife used).... } } Perhaps there is a simple solution......who knows, } } } best regards and have a nice weekend, } } Wolfgang Muss } } Salzburg, Austria } } Paracelsus Medical Private University (PMU) } Institute of Pathology } Electron Microscopy Lab } Muellner Hauptstrasse 48 } A-5020 SALZBURG, Austria/Europe } Phone work: +43+662+4482+4720 } Mobile phone work:+43+662+4482-57704 } Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o } W.Muss") } E-Mail work: W.Muss-at-SALK.at } } Mobile-phone private: ++43+676+5 369-456 } E-Mail private: wij.Muss-at-aon.at } ------------------------------------------------------------------------ } --------------------------------Information on behalf of } Society for Cutaneous Ultrastructure Research (SCUR) } PLEASE VISIT THE UPDATED WEBSITE of SCUR at } } http://www.scur.org { } ------------------------------------------------------------------------- } Forthcoming Meetings: } 33rd Annual Meeting of the SCUR, 8th-10th June, 2006, WARSZAW, Poland } WEBSITE, containing all FORMS: http://www.scur.org.pl } Additional informations: send an E-Mail } kwoznia-at-amwaw.edu.pl } ---------------------- } 34th Annual Meeting of the SCUR, 11th-12th May, 2007, PRAGUE } Czech Republic } ---------------------- } 35th Annual Meeting of the SCUR, 11th -12th May, 2008, NARA or KYOTO, Japan } Joint Meeting with the } JSUCB, the Japanese Society for Ultrastructural Cutaneous Biology } } } } } } } ---------- } Von: GBurgess-at-exchange.hsc.mb.ca[SMTP:GBurgess-at-exchange.hsc.mb.ca] } Antwort an: GBurgess-at-exchange.hsc.mb.ca } Gesendet: Freitag, 20. Janner 2006 23:52 } An: W.Muss-at-salk.at } Betreff: [Microscopy] Wrinkled LM Sections } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } Even though I am very experienced in Electron Microscopy, there are times } when I cut LM sections that are extremely wrinkled, and they look like } cracked glass. I normally don't experience this, but when it happens, even } considering every possible variable, I'm still not exactly sure what might } be at work here to give this sort of result. } } I'm looking for ideas here as to what might be causing this effect. What } do } people think? } } } Garry Burgess } Charge Technologist - Electron Microscopy } Health Sciences Centre } Winnipeg, Canada. } } This e-mail and/or any documents in this transmission is intended for the } address(s) only and may contain legally privileged or confidential } information. Any unauthorized use, disclosure, distribution, copying or } dissemination is strictly prohibited. If you receive this transmission in } error, please notify the sender immediately and return the original. } } ==============================Original } Headers============================== } 6, 20 -- From GBurgess-at-exchange.hsc.mb.ca Fri Jan 20 16:10:02 2006 } 6, 20 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca } [142.233.100.122]) } 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } k0KMA1mn017769 } 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Jan 2006 16:10:01 -0600 } 6, 20 -- Received: from hscxntmx0007.hsc.mb.ca (unverified [172.16.6.179]) } by } 6, 20 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS 4.0.346.0) with ESMTP id } 6, 20 -- {B0017584293-at-hscxntmx0003.hsc.mb.ca} for } {Microscopy-at-microscopy.com} ;Fri, } 6, 20 -- 20 Jan 2006 16:09:53 -0600 } 6, 20 -- Received: by hscxntmx0007.hsc.mb.ca with Internet Mail Service } 6, 20 -- (5.5.2653.19)id {T7CLC1WX} ; Fri, 20 Jan 2006 16:10:27 -0600 } 6, 20 -- Message-ID: } 6, 20 -- {00A937989100304A83A058F6C45873FF32A36C-at-hscxntmx0005.hsc.mb.ca} } 6, 20 -- Date: Fri, 20 Jan 2006 16:06:48 -0600 } 6, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} } 6, 20 -- Subject: Wrinkled LM Sections } 6, 20 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 6, 20 -- X-Mailer: Internet Mail Service (5.5.2653.19) } 6, 20 -- MIME-Version: 1.0 } 6, 20 -- Content-Type: text/plain; } 6, 20 -- charset="iso-8859-1" } ==============================End of - } Headers============================== } } } } ==============================Original Headers============================== } 25, 28 -- From W.Muss-at-salk.at Sat Jan 21 04:43:58 2006 } 25, 28 -- Received: from Hermes.salk.at (hermes.lks.at [193.170.167.9]) } 25, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0LAhv9E003479 } 25, 28 -- for {Microscopy-at-microscopy.com} ; Sat, 21 Jan 2006 04:43:57 -0600 } 25, 28 -- Received: from localhost (localhost [127.0.0.1]) } 25, 28 -- by hermes.lks.at (Postfix) with ESMTP id B185A5A9046; } 25, 28 -- Sat, 21 Jan 2006 11:43:51 +0100 (CET) } 25, 28 -- Received: from hermes.lks.at ([127.0.0.1]) } 25, 28 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024) } 25, 28 -- with ESMTP id 42900-09; Sat, 21 Jan 2006 11:43:51 +0100 (CET) } 25, 28 -- Received: from c1pa003 (unknown [192.168.42.3]) } 25, 28 -- by hermes.lks.at (Postfix) with SMTP id 754D65A9044; } 25, 28 -- Sat, 21 Jan 2006 11:43:51 +0100 (CET) } 25, 28 -- Received: by localhost with Microsoft MAPI; Sat, 21 Jan 2006 11:43:49 +0100 } 25, 28 -- Message-ID: {01C61E7F.F216D3E0.W.Muss-at-salk.at} } 25, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at} } 25, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at} } 25, 28 -- To: "'GBurgess-at-exchange.hsc.mb.ca'" {GBurgess-at-exchange.hsc.mb.ca} , } 25, 28 -- "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 25, 28 -- Subject: [Microscopy] Re: Wrinkled LM Sections } 25, 28 -- Date: Sat, 21 Jan 2006 11:43:48 +0100 } 25, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at} } 25, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor } 25, 28 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211 } 25, 28 -- MIME-Version: 1.0 } 25, 28 -- Content-Type: text/plain; charset="us-ascii" } 25, 28 -- Content-Transfer-Encoding: 7bit } 25, 28 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 25 -- From nairvinods-at-gmail.com Sun Jan 22 17:45:05 2006 6, 25 -- Received: from zproxy.gmail.com (zproxy.gmail.com [64.233.162.195]) 6, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0MNj4D3022636 6, 25 -- for {microscopy-at-microscopy.com} ; Sun, 22 Jan 2006 17:45:04 -0600 6, 25 -- Received: by zproxy.gmail.com with SMTP id 9so805621nzo 6, 25 -- for {microscopy-at-microscopy.com} ; Sun, 22 Jan 2006 15:45:03 -0800 (PST) 6, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 25 -- s=beta; d=gmail.com; 6, 25 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 6, 25 -- b=iwLI/yOhyn8RBFdLsd4GrjjADMj8yG6x8a+cZRddAPXP313KWFTH8mhP3WJuqdwmBf56pLTJ6QHi6KG7BMIsRtxOEYY4fvwY5oFvcx+g2Qe17GhH6Q6gSFUGuaU44HZYNriuBxtZcmHsPNQ9H4Cu/ddrt5xiiMyNw1HrJ33u1+I= 6, 25 -- Received: by 10.64.251.4 with SMTP id y4mr2436900qbh; 6, 25 -- Sun, 22 Jan 2006 15:38:52 -0800 (PST) 6, 25 -- Received: by 10.64.201.14 with HTTP; Sun, 22 Jan 2006 15:38:52 -0800 (PST) 6, 25 -- Message-ID: {ea42a3900601221538n63c2c777pab547602772aa678-at-mail.gmail.com} 6, 25 -- Date: Sun, 22 Jan 2006 16:38:52 -0700 6, 25 -- From: Vinod Nair {nairvinods-at-gmail.com} 6, 25 -- To: microscopy-at-microscopy.com 6, 25 -- Subject: Re: [Microscopy] Re: Wrinkled LM Sections 6, 25 -- In-Reply-To: {200601211126.k0LBQBcT011510-at-ns.microscopy.com} 6, 25 -- MIME-Version: 1.0 6, 25 -- Content-Type: text/plain; charset=UTF-8 6, 25 -- Content-Disposition: inline 6, 25 -- References: {200601211126.k0LBQBcT011510-at-ns.microscopy.com} 6, 25 -- Content-Transfer-Encoding: 8bit 6, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id k0MNj4D3022636 ==============================End of - Headers==============================
Mei Lie, Making formvar films in ethylene dichloride We used these films for 30 yrs and controlled the thickness mostly by the percentage of formvar to ethylene dichloride. What percentage are you using?
Judy
Judy Murphy, PhD Microscopy Training, Imaging, and Lab Design Stockton, CA 95219 murphyjudy-at-comcast.net
wong-at-msg.ucsf.edu wrote:
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==============================Original Headers============================== 12, 19 -- From murphyjudy-at-comcast.net Sun Jan 22 22:53:43 2006 12, 19 -- Received: from sccrmhc12.comcast.net (sccrmhc12.comcast.net [63.240.77.82]) 12, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0N4rhWv009022 12, 19 -- for {microscopy-at-microscopy.com} ; Sun, 22 Jan 2006 22:53:43 -0600 12, 19 -- Received: from [192.168.1.102] (c-67-181-78-13.hsd1.ca.comcast.net[67.181.78.13]) 12, 19 -- by comcast.net (sccrmhc12) with ESMTP 12, 19 -- id {20060123045340012005vqqbe} ; Mon, 23 Jan 2006 04:53:41 +0000 12, 19 -- Message-ID: {43D46116.2070908-at-comcast.net} 12, 19 -- Date: Sun, 22 Jan 2006 20:52:38 -0800 12, 19 -- From: Judy Murphy {murphyjudy-at-comcast.net} 12, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 12, 19 -- X-Accept-Language: en-us, en 12, 19 -- MIME-Version: 1.0 12, 19 -- To: Microscopy List Server {microscopy-at-microscopy.com} 12, 19 -- Subject: Re: [Microscopy] viaWWW: Making formvar thicker 12, 19 -- References: {200601211425.k0LEP0O9019151-at-ns.microscopy.com} 12, 19 -- In-Reply-To: {200601211425.k0LEP0O9019151-at-ns.microscopy.com} 12, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
A web site was brought to my attention that is a collection of photographs by him (and several of him) during Del's time with Albert Szent-Gyorgyi. I found it very interesting and rich in the history of science.
Thank you Peter for sharing the information AND hold on to that signed copy of Crossing the Elbe!
murphyjudy-at-comcast.net wrote:
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==============================Original Headers============================== 8, 19 -- From murphyjudy-at-comcast.net Sun Jan 22 23:08:21 2006 8, 19 -- Received: from sccrmhc14.comcast.net (sccrmhc14.comcast.net [63.240.77.84]) 8, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0N58KXw011747 8, 19 -- for {microscopy-at-microscopy.com} ; Sun, 22 Jan 2006 23:08:20 -0600 8, 19 -- Received: from [192.168.1.102] (c-67-181-78-13.hsd1.ca.comcast.net[67.181.78.13]) 8, 19 -- by comcast.net (sccrmhc14) with ESMTP 8, 19 -- id {2006012305081301400kjrige} ; Mon, 23 Jan 2006 05:08:14 +0000 8, 19 -- Message-ID: {43D464BD.4010206-at-comcast.net} 8, 19 -- Date: Sun, 22 Jan 2006 21:08:13 -0800 8, 19 -- From: Judy Murphy {murphyjudy-at-comcast.net} 8, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 8, 19 -- X-Accept-Language: en-us, en 8, 19 -- MIME-Version: 1.0 8, 19 -- To: Microscopy List Server {microscopy-at-microscopy.com} 8, 19 -- Subject: Re: [Microscopy] We Remember Del Philpott 8, 19 -- References: {200601222350.k0MNoOo1024081-at-ns.microscopy.com} 8, 19 -- In-Reply-To: {200601222350.k0MNoOo1024081-at-ns.microscopy.com} 8, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi: I am restoring a Zeiss 109 TEM where the Leybold IZ-80 iongetter pump has been removed, however they left the magnets. The pump does not have to be in working condition. If you have on lying around and are willing to part with it or sell it please let me know. Peter Jordan/EMSI
==============================Original Headers============================== 2, 22 -- From pjordan-at-dslextreme.com Sun Jan 22 23:53:27 2006 2, 22 -- Received: from mail5.dslextreme.com (mail5.dslextreme.com [66.51.199.81]) 2, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k0N5rQiS023856 2, 22 -- for {microscopy-at-msa.microscopy.com} ; Sun, 22 Jan 2006 23:53:26 -0600 2, 22 -- Received: (qmail 30488 invoked from network); 23 Jan 2006 05:52:12 -0000 2, 22 -- Received: from unknown (HELO DCPZ5N31) (68.183.94.207) 2, 22 -- by mail5.dslextreme.com with SMTP; Sun, 22 Jan 2006 21:52:12 -0800 2, 22 -- Message-ID: {005401c61fe1$5218af90$cf5eb744-at-DCPZ5N31} 2, 22 -- From: "Peter Jordan" {pjordan-at-dslextreme.com} 2, 22 -- To: "Electron Microscopy" {Microscopy-at-msa.microscopy.com} 2, 22 -- Subject: Iongetter pump for Zeiss 109 2, 22 -- Date: Sun, 22 Jan 2006 21:53:22 -0800 2, 22 -- MIME-Version: 1.0 2, 22 -- Content-Type: text/plain; 2, 22 -- format=flowed; 2, 22 -- charset="iso-8859-1"; 2, 22 -- reply-type=original 2, 22 -- Content-Transfer-Encoding: 7bit 2, 22 -- X-Priority: 3 2, 22 -- X-MSMail-Priority: Normal 2, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 2, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
Dear Gary, Let me begin with apologising for not reading the email correctly. You can see what I have been pondering over from my reply with regards to LR white resin:( Guess it was desperation that made me misread LM as LR and link it to the wrinkeling problems I have been facing. Having said that I feel very foolish now for being hasty and replying to your email query. My sincere apologies.
But then again I am open for suggestions,comments or insight into the problem I am facing. regards, Vinod
==============================Original Headers============================== 3, 25 -- From nairvinods-at-gmail.com Mon Jan 23 00:03:21 2006 3, 25 -- Received: from wproxy.gmail.com (wproxy.gmail.com [64.233.184.196]) 3, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0N63JfS026967 3, 25 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2006 00:03:20 -0600 3, 25 -- Received: by wproxy.gmail.com with SMTP id i30so857046wra 3, 25 -- for {microscopy-at-microscopy.com} ; Sun, 22 Jan 2006 22:03:19 -0800 (PST) 3, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 3, 25 -- s=beta; d=gmail.com; 3, 25 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 3, 25 -- b=qLDCg7ZEROXSfD8aXh3sQ7gV08fkTVY98jzE998c6AOreIritxcbW8wfosf9yDu9vRIfZK7mOZoljeb0to6Wf+TmtvFGfObfREqnJAWvRbc9PTSIm6Ow+fqo0HEEIcqZzzbUyMrn61hqiKideomsMmSKQsDqs0bm8+Hne14//ZM= 3, 25 -- Received: by 10.64.143.19 with SMTP id q19mr271269qbd; 3, 25 -- Sun, 22 Jan 2006 21:57:15 -0800 (PST) 3, 25 -- Received: by 10.64.201.14 with HTTP; Sun, 22 Jan 2006 21:57:15 -0800 (PST) 3, 25 -- Message-ID: {ea42a3900601222157v6fe56138p3a4d08b1e4b0777a-at-mail.gmail.com} 3, 25 -- Date: Sun, 22 Jan 2006 22:57:15 -0700 3, 25 -- From: Vinod Nair {nairvinods-at-gmail.com} 3, 25 -- To: microscopy-at-microscopy.com 3, 25 -- Subject: Re: [Microscopy] Wrinkled LM Sections 3, 25 -- In-Reply-To: {200601230040.k0N0elR9004151-at-ns.microscopy.com} 3, 25 -- MIME-Version: 1.0 3, 25 -- Content-Type: text/plain; charset=UTF-8 3, 25 -- Content-Disposition: inline 3, 25 -- References: {200601230040.k0N0elR9004151-at-ns.microscopy.com} 3, 25 -- Content-Transfer-Encoding: 8bit 3, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id k0N63JfS026967 ==============================End of - Headers==============================
On Jan 21, 2006, at 6:03 AM, wong-at-msg.ucsf.edu wrote:
} Question: When using formvar in ethylene dichloride, is the any way to } make the films slightly thicker. I know when using formvar in } chloroform, you can just drain slower or faster to make films thinner } or thicker. Is there something like this for the ethylene dichloride } formula? I have tried several different times but so far haven't hit } on anything totally satisfactory. } Dear Mei Lie, Using a more concentrated formvar solution should do it, and if you only have the pre-disolved formvar, you could try either letting some of the solvent evaporate or double dipping. For finer variations you might try letting the formvar drain at a shallower angle, which will drain it slightly slower. Good luck. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Mon Jan 23 11:37:40 2006 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0NHbemg016835 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 23 Jan 2006 11:37:40 -0600 4, 22 -- Received: from localhost (fire-dog [192.168.1.4]) 4, 22 -- by earth-ox-postvirus (Postfix) with ESMTP id 7071C109F3B 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 23 Jan 2006 09:37:39 -0800 (PST) 4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 4, 22 -- by earth-ox.its.caltech.edu (Postfix) with ESMTP id E6173109E19 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 23 Jan 2006 09:37:37 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v623) 4, 22 -- In-Reply-To: {200601211403.k0LE3NrZ014761-at-ns.microscopy.com} 4, 22 -- References: {200601211403.k0LE3NrZ014761-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {6675a89478c5c6f5c36fc2d5e8dcd7a0-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] viaWWW: Making formvar thicker 4, 22 -- Date: Mon, 23 Jan 2006 09:44:45 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.623) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.3.3 ==============================End of - Headers==============================
Dear All, We have an older Zeiss Opni-1 surgical microscope and we'd like to replace its broken tungsten illuminator with a fiber optic illuminator. this would preserve the large illuminated field diameter and be adjustable in angle. the usual web searches have shown that retrofits were made for this scope, but haven't provided any useful information on performance or options. It is already used in conjunction with bifurcated light guides, but we have users who need the stock style of illumination.
Thanks, Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
************************************************************************ ****** The box said "Requires Windows 95 or better", so I bought a Macintosh. ************************************************************************ ******
==============================Original Headers============================== 7, 23 -- From glenmac-at-u.washington.edu Mon Jan 23 11:39:38 2006 7, 23 -- Received: from mxout2.cac.washington.edu (mxout2.cac.washington.edu [140.142.33.4]) 7, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0NHdbYl017204 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 11:39:37 -0600 7, 23 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.139]) 7, 23 -- by mxout2.cac.washington.edu (8.13.5+UW05.10/8.13.5+UW05.09) with ESMTP id k0NHdaNw018071 7, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 09:39:37 -0800 7, 23 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) 7, 23 -- (authenticated authid=glenmac) 7, 23 -- by smtp.washington.edu (8.13.5+UW05.10/8.13.5+UW05.09) with ESMTP id k0NHdac2004853 7, 23 -- (version=TLSv1/SSLv3 cipher=RC4-SHA bits=128 verify=NOT) 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 09:39:36 -0800 7, 23 -- Mime-Version: 1.0 (Apple Message framework v746.2) 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- Message-Id: {E3E326B4-892B-4DAE-BA4A-8D7CA81A632C-at-u.washington.edu} 7, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 23 -- To: MSA {Microscopy-at-MSA.Microscopy.Com} 7, 23 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 7, 23 -- Subject: fiber illuminator for Opni-1 7, 23 -- Date: Mon, 23 Jan 2006 09:39:32 -0800 7, 23 -- X-Mailer: Apple Mail (2.746.2) 7, 23 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_CRUFT 0, __STOCK_PHRASE_6 0' ==============================End of - Headers==============================
I need to measure thermal conductivity of thin, 50-200 nm film. I would appreciate any suggestions regarding to equipment, techniques, and literature references.
Thank you,
Victoria Fink vfink-at-shaw.ca
==============================Original Headers============================== 7, 28 -- From vfink-at-shaw.ca Mon Jan 23 12:04:15 2006 7, 28 -- Received: from pd5mo1so.prod.shaw.ca (shawidc-mo1.cg.shawcable.net [24.71.223.10]) 7, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0NI4FmR023679 7, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 12:04:15 -0600 7, 28 -- Received: from pd2mr7so.prod.shaw.ca (pd2mr7so-qfe3.prod.shaw.ca [10.0.141.10]) 7, 28 -- by l-daemon (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 15 2004)) 7, 28 -- with ESMTP id {0ITK00H9N4UXI290-at-l-daemon} for Microscopy-at-MSA.Microscopy.Com; 7, 28 -- Mon, 23 Jan 2006 11:04:09 -0700 (MST) 7, 28 -- Received: from pn2ml1so.prod.shaw.ca ([10.0.121.145]) 7, 28 -- by pd2mr7so.prod.shaw.ca (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 7, 28 -- 15 2004)) with ESMTP id {0ITK00JKF4UX7300-at-pd2mr7so.prod.shaw.ca} for 7, 28 -- Microscopy-at-MSA.Microscopy.Com; Mon, 23 Jan 2006 11:04:09 -0700 (MST) 7, 28 -- Received: from homep5whcwsdwx ([70.68.254.15]) 7, 28 -- by l-daemon (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 15 2004)) 7, 28 -- with SMTP id {0ITK001EW4UVGR10-at-l-daemon} for Microscopy-at-MSA.Microscopy.Com; 7, 28 -- Mon, 23 Jan 2006 11:04:09 -0700 (MST) 7, 28 -- Date: Mon, 23 Jan 2006 10:04:11 -0800 7, 28 -- From: Victoria Fink {vfink-at-shaw.ca} 7, 28 -- Subject: thermal conductivity of thin film measurement techniques 7, 28 -- To: MSA listserver {Microscopy-at-MSA.Microscopy.Com} 7, 28 -- Message-id: {042001c62047$69d10270$0ffe4446-at-homep5whcwsdwx} 7, 28 -- MIME-version: 1.0 7, 28 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 7, 28 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 7, 28 -- Content-type: text/plain; format=flowed; charset=iso-8859-1; reply-type=response 7, 28 -- Content-transfer-encoding: 7bit 7, 28 -- X-Priority: 3 7, 28 -- X-MSMail-priority: Normal ==============================End of - Headers==============================
Inked to measure thermal conductivity of thin, 50-200 nm film. I would appreciate any suggestions regarding to equipment, techniques, and literature references. Thank you,
Victoria Fink vfink-at-shaw.ca
==============================Original Headers============================== 6, 29 -- From vfink-at-shaw.ca Mon Jan 23 12:04:23 2006 6, 29 -- Received: from pd4mo1so.prod.shaw.ca (shawidc-mo1.cg.shawcable.net [24.71.223.10]) 6, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0NI4L6j023721 6, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 12:04:22 -0600 6, 29 -- Received: from pd3mr2so.prod.shaw.ca 6, 29 -- (pd3mr2so-qfe3.prod.shaw.ca [10.0.141.178]) by l-daemon 6, 29 -- (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 15 2004)) 6, 29 -- with ESMTP id {0ITK00MFH4TCBF80-at-l-daemon} for Microscopy-at-MSA.Microscopy.Com; 6, 29 -- Mon, 23 Jan 2006 11:03:12 -0700 (MST) 6, 29 -- Received: from pn2ml1so.prod.shaw.ca ([10.0.121.145]) 6, 29 -- by pd3mr2so.prod.shaw.ca (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 6, 29 -- 15 2004)) with ESMTP id {0ITK00BYS4TCXT10-at-pd3mr2so.prod.shaw.ca} for 6, 29 -- Microscopy-at-MSA.Microscopy.Com; Mon, 23 Jan 2006 11:03:12 -0700 (MST) 6, 29 -- Received: from homep5whcwsdwx ([70.68.254.15]) 6, 29 -- by l-daemon (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 15 2004)) 6, 29 -- with SMTP id {0ITK00FRN4TAXA50-at-l-daemon} for Microscopy-at-MSA.Microscopy.Com; 6, 29 -- Mon, 23 Jan 2006 11:03:12 -0700 (MST) 6, 29 -- Date: Mon, 23 Jan 2006 10:03:14 -0800 6, 29 -- From: Victoria Fink {vfink-at-shaw.ca} 6, 29 -- Subject: thermal conductivity of thin film measurement techniques 6, 29 -- To: MSA listserver {Microscopy-at-MSA.Microscopy.Com} 6, 29 -- Message-id: {041a01c62047$47e848d0$0ffe4446-at-homep5whcwsdwx} 6, 29 -- MIME-version: 1.0 6, 29 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 6, 29 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 6, 29 -- Content-type: text/plain; format=flowed; charset=iso-8859-1; reply-type=original 6, 29 -- Content-transfer-encoding: 7bit 6, 29 -- X-Priority: 3 6, 29 -- X-MSMail-priority: Normal ==============================End of - Headers==============================
A number of our customers for coated grids request a variety of thicknesses. What we do for thicker coated grids is increase the percentage of formvar in ethylene dichloride.
Mike Bouchard
Disclaimer: Ladd Research sells Formvar coated grids and the essentials to make your own.
At 09:46 AM 1/21/2006, wong-at-msg.ucsf.edu wrote:
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I am curious about the possible skinkage artifact that may or may not occur when cryoprotecting lightly fixed (4% para / 0.1% glut) watery tissues (embryonic skin) in 20% PVP / 1.7M sucrose for ultrathin cryomicrotomy? Does the cryoprotectant replace the water in a controlled way and keep the tissue fat and happy or does it draw the water out and collapse the tissue? I would love any information on this subject or a possible reference.
Robert Underwood University of Washington Dermatology
==============================Original Headers============================== 4, 20 -- From underwoo-at-u.washington.edu Mon Jan 23 13:08:13 2006 4, 20 -- Received: from mxout7.cac.washington.edu (mxout7.cac.washington.edu [140.142.32.178]) 4, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0NJ8Anx013006 4, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 13:08:11 -0600 4, 20 -- Received: from hymn02.u.washington.edu (hymn02.u.washington.edu [140.142.13.239]) 4, 20 -- by mxout7.cac.washington.edu (8.13.5+UW05.10/8.13.5+UW05.09) with ESMTP id k0NJ89DN018919 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 11:08:09 -0800 4, 20 -- Received: from localhost (localhost [127.0.0.1]) 4, 20 -- by hymn02.u.washington.edu (8.13.5+UW05.10/8.13.5+UW05.09) with ESMTP id k0NJ894u023894 4, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 11:08:09 -0800 4, 20 -- X-Auth-Received: from [128.208.106.163] by hymn02.u.washington.edu via HTTP; Mon, 23 Jan 2006 11:08:09 PST 4, 20 -- Date: Mon, 23 Jan 2006 11:08:09 -0800 (PST) 4, 20 -- From: Robert A Underwood {underwoo-at-u.washington.edu} 4, 20 -- To: Microscopy List {Microscopy-at-MSA.Microscopy.Com} 4, 20 -- Subject: [Microscopy] Cryoprotection causing shrinkage artifact 4, 20 -- Message-ID: {Pine.LNX.4.43.0601231108090.3305-at-hymn02.u.washington.edu} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 4, 20 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
Postdoctoral Position, Department of Materials Science and Engineering Lehigh University,
Available March 2006. Opening for post-doc to work on project involving processing of patterned sapphire substrates via oxidation and solid state conversion of a metallic Al coating. Person would be responsible for processing and EBSD/TEM characterization of both the substrates, together with TEM of MOCVD grown GaN layers to determine the defect density. Expertise in materials processing and electron microscopy required, experience with thin film processing a plus. Please forward resume by e-mail to Prof. Helen Chan (Helen.Chan-at-lehigh.edu) and / or Prof. Richard Vinci (rpv2-at-lehigh.edu).
...........
Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195
==============================Original Headers============================== 5, 22 -- From jae5-at-lehigh.edu Mon Jan 23 13:25:44 2006 5, 22 -- Received: from rain.CC.Lehigh.EDU (rain.CC.Lehigh.EDU [128.180.39.20]) 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0NJPhoS019690 5, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2006 13:25:44 -0600 5, 22 -- Received: from [128.180.55.141] (Dyn055141.mat.Lehigh.EDU [128.180.55.141]) 5, 22 -- (authenticated bits=0) 5, 22 -- by rain.CC.Lehigh.EDU (8.13.5/8.13.5) with ESMTP id k0NJPhEc031735 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 5, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2006 14:25:43 -0500 5, 22 -- Message-ID: {43D52DB7.9050707-at-lehigh.edu} 5, 22 -- Date: Mon, 23 Jan 2006 14:25:43 -0500 5, 22 -- From: Alwyn Eades {jae5-at-lehigh.edu} 5, 22 -- Organization: Lehigh University 5, 22 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 5, 22 -- X-Accept-Language: en-us, en 5, 22 -- MIME-Version: 1.0 5, 22 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com} 5, 22 -- Subject: Post doctoral position at Lehigh 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-Virus-Scanned: ClamAV version 0.88, clamav-milter version 0.87 on rain.CC.Lehigh.EDU 5, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I have a colleague who recently experienced some down time on his SEM. The system went through repeated cycles of pump down, and venting and was eventually left powered down while waiting on a computer replacement.
After getting everything operating again, an oil film was found that had built up on the x-ray detector thin window. This was initially causing an unacceptable amount of background in the 0-3kV range of the x-ray signal as well as an overall low count rate. The collimator was removed and cleaned, which corrected the noise and count rate. They are in the process of replacing the roughing lines and cleaning the chamber to prevent further contamination. Now, when calibrating with a copper standard, the ratio of the L line signal versus the K lines is dramatically favoring the L. This was not the case before.
Any ideas why this might be happening and how to correct it?
Karl Hagglund Biological Sciences, SC-102B Northern Kentucky University, Nunn Drive Highland Heights, KY 41099 859-572-5238 http://semlab.nku.edu
==============================Original Headers============================== 5, 23 -- From hagglundk1-at-nku.edu Tue Jan 24 09:02:19 2006 5, 23 -- Received: from mailfe2.nku.edu (mailfe2.nku.edu [192.122.237.68]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0OF2JlW001861 5, 23 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2006 09:02:19 -0600 5, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211); 5, 23 -- Tue, 24 Jan 2006 10:02:19 -0500 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 5, 23 -- Content-class: urn:content-classes:message 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="us-ascii" 5, 23 -- Subject: SEM x-ray peak ratios 5, 23 -- Date: Tue, 24 Jan 2006 10:02:18 -0500 5, 23 -- Message-ID: {F01E4499C4EC5842A8AB7198141AC72F3585FA-at-mailfac1.hh.nku.edu} 5, 23 -- X-MS-Has-Attach: 5, 23 -- X-MS-TNEF-Correlator: 5, 23 -- Thread-Topic: SEM x-ray peak ratios 5, 23 -- Thread-Index: AcYg9yuMm942rGb1QfKCevfjxyMD/g== 5, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu} 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- X-OriginalArrivalTime: 24 Jan 2006 15:02:19.0222 (UTC) FILETIME=[2BE72F60:01C620F7] 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0OF2JlW001861 ==============================End of - Headers==============================
Does anyone remember a quick and easy way to calibrate - it has what 25 taps - so if anyone remembers how to do the calculations, please let me know ASAP. Thanks Barbara
==============================Original Headers============================== 1, 20 -- From maloneyb-at-fiu.edu Tue Jan 24 13:40:30 2006 1, 20 -- Received: from imappopsmtp.fiu.edu (imappopsmtp.fiu.edu [131.94.74.203]) 1, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0OJeTel015388 1, 20 -- for {microscopy-at-sparc5.microscopy.com} ; Tue, 24 Jan 2006 13:40:30 -0600 1, 20 -- Received: from [131.94.95.156] by imappopsmtp.fiu.edu 1, 20 -- (InterMail vK.4.04.00.00 201-232-137 license dcade68804da15b88628cbdb48d26a54) 1, 20 -- with SMTP 1, 20 -- id {20060124194058.VXOU22222.imappopsmtp-at-[131.94.95.156]} 1, 20 -- for {microscopy-at-sparc5.microscopy.com} ; 1, 20 -- Tue, 24 Jan 2006 14:40:58 -0500 1, 20 -- Message-ID: {43D68175.1080106-at-fiu.edu} 1, 20 -- Date: Tue, 24 Jan 2006 14:35:17 -0500 1, 20 -- From: Barbara Maloney {maloneyb-at-fiu.edu} 1, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 1, 20 -- X-Accept-Language: en-us, en 1, 20 -- MIME-Version: 1.0 1, 20 -- To: "'microscopy-at-msa.microscopy.com'" {microscopy-at-ns.microscopy.com} 1, 20 -- Subject: Phillips 300 TEM calibration 1, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed 1, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have had a request from a user looking for a cryo- ultramicrotomy workshop. Is anyone offering one in the next year or know of one? Thanks!
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"WE ARE MICROSOFT. RESISTANCE IS FUTILE. YOU WILL BE ASSIMILATED."
==============================Original Headers============================== 5, 23 -- From edelmare-at-muohio.edu Tue Jan 24 14:11:13 2006 5, 23 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0OKBBkX020625 5, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 24 Jan 2006 14:11:12 -0600 5, 23 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 5, 23 -- by mulnx12.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id k0OKB14j016596 5, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 24 Jan 2006 15:11:01 -0500 5, 23 -- Received: from emf03 ([134.53.14.97]) 5, 23 -- by mulnx24.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id k0OKB002022369 5, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 24 Jan 2006 15:11:00 -0500 5, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 5, 23 -- To: microscopy-at-Microscopy.com 5, 23 -- Date: Tue, 24 Jan 2006 15:11:49 -0500 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-type: text/plain; charset=US-ASCII 5, 23 -- Content-transfer-encoding: 7BIT 5, 23 -- Subject: Cryo-ultramicrotomy workshop? 5, 23 -- Message-ID: {43D643B5.14764.643E209-at-localhost} 5, 23 -- Priority: normal 5, 23 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 5, 23 -- X-Real-ConnectIP: 134.53.14.97 5, 23 -- X-Scanned-By: MIMEDefang 2.45 5, 23 -- X-Scanned-By: MIMEDefang 2.52 on 134.53.6.11 ==============================End of - Headers==============================
Several years ago, Emispec Systems was purchased by FEI Company of Hillsboro, OR. Recently I've been contacted by several customers who in the past had purchased these data acquisition systems for their EM's and stated they are having difficulty making contact with the responsible person or department at FEI for upgrades and maintenance support.
I'm wondering if parties who are present owners of Emispec ES Vision systems have had success in seeking this support and can provide contact information or advise of appropriate channels to go through to obtain system support.
Please respond off list if you can shed some light on this.
Regards,
Bob Roberts EM Lab Services, Inc. 449 NW 62nd St. Topeka, Kansas 66617-1780 785.246.1232 voice 785.246.0168 fax www.emlabservices.com
==============================Original Headers============================== 8, 23 -- From emlabservices-at-cox.net Tue Jan 24 16:34:05 2006 8, 23 -- Received: from centrmmtao05.cox.net (centrmmtao05.cox.net [70.168.83.79]) 8, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0OMY4Mt004289 8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Jan 2006 16:34:05 -0600 8, 23 -- Received: from EMLabServices ([24.255.213.54]) by centrmmtao05.cox.net 8, 23 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with SMTP 8, 23 -- id {20060124223407.SQDC5868.centrmmtao05.cox.net-at-EMLabServices} 8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Jan 2006 17:34:07 -0500 8, 23 -- Message-ID: {012701c62136$49ad42c0$6400a8c0-at-EMLabServices} 8, 23 -- From: "EM Lab Services" {emlabservices-at-cox.net} 8, 23 -- To: {Microscopy-at-microscopy.com} 8, 23 -- Subject: Emispec Systems/FEI Support? 8, 23 -- Date: Tue, 24 Jan 2006 15:34:06 -0700 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- format=flowed; 8, 23 -- charset="iso-8859-1"; 8, 23 -- reply-type=original 8, 23 -- Content-Transfer-Encoding: 7bit 8, 23 -- X-Priority: 3 8, 23 -- X-MSMail-Priority: Normal 8, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 8, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (fmalherbe-at-swin.edu.au) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, January 24, 2006 at 18:23:45 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sallwein-at-ncifcrf.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Employment Opportunity to work in Support of the National Cancer Institute at SAIC-Frederick, Inc.
Question: SAIC-Frederick, Inc., working in support of the National Cancer Institute at Frederick, seeks an experienced Research Associate to be responsible for independent imaging TEM and SEM samples in a core facility, processing samples and coordinating GLP samples in the lab, processing, sectioning and imaging core samples, training, and conduct collaborative projects with the National Cancer Institute and the National Institutes of Health. Position requires a BS degree and at least four years of experience with TEM and/or SEM sample processing and imaging.
Please visit our web site at http://saic.ncifcrf.gov and refer to opportunity KMB134306 to apply on line.
==============================Original Headers============================== 7, 13 -- From zaluzec-at-microscopy.com Wed Jan 25 07:34:03 2006 7, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 7, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0PDY1FL024339 7, 13 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jan 2006 07:34:03 -0600 7, 13 -- Mime-Version: 1.0 7, 13 -- X-Sender: (Unverified) 7, 13 -- Message-Id: {p06110406bffd2eaa5f0c-at-[206.69.208.22]} 7, 13 -- Date: Wed, 25 Jan 2006 07:34:00 -0600 7, 13 -- To: microscopy-at-microscopy.com 7, 13 -- From: sallwein-at-ncifcrf.gov (by way of MicroscopyListserver) 7, 13 -- Subject: [Filtered] MicroscopyListserverviaWWW: Employment Opportunity to 7, 13 -- work in Support of the National Cancer Institute at SAIC-Frederick 7, 13 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
The 5th International Cryo-EM Course is I believe June 6-17, 2006 in Vancouver, Canada at University of British Columbia by Dr. Elaine Humphrey from UBC and Dr. Kent McDonald, from UC, Berkeley with international speakers. This is an excellent course and caters to the needs of the particular students. Be sure to check with Elaine about dates. For more info email Dr. Elaine Humphrey ech-at-interchange.ubc.ca
Information from the previous course is listed at http://www.emlab.ubc.ca/CryoEM2005/index.html
Judy
Judy Murphy, PhD Microscopy Training, Imaging, and Lab Design Stockton, CA 95219 murphyjudy-at-comcast.net
edelmare-at-muohio.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 14, 19 -- From murphyjudy-at-comcast.net Wed Jan 25 09:51:50 2006 14, 19 -- Received: from sccrmhc14.comcast.net (sccrmhc14.comcast.net [63.240.77.84]) 14, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0PFpnJP012033 14, 19 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jan 2006 09:51:50 -0600 14, 19 -- Received: from [192.168.1.102] (c-67-181-78-13.hsd1.ca.comcast.net[67.181.78.13]) 14, 19 -- by comcast.net (sccrmhc14) with ESMTP 14, 19 -- id {2006012515514401400eiimne} ; Wed, 25 Jan 2006 15:51:44 +0000 14, 19 -- Message-ID: {43D79E99.2010905-at-comcast.net} 14, 19 -- Date: Wed, 25 Jan 2006 07:51:53 -0800 14, 19 -- From: Judy Murphy {murphyjudy-at-comcast.net} 14, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 14, 19 -- X-Accept-Language: en-us, en 14, 19 -- MIME-Version: 1.0 14, 19 -- To: edelmare-at-muohio.edu, Microscopy List Server {microscopy-at-microscopy.com} 14, 19 -- Subject: Re: [Microscopy] Cryo-ultramicrotomy workshop? 14, 19 -- References: {200601242046.k0OKklJE029987-at-ns.microscopy.com} 14, 19 -- In-Reply-To: {200601242046.k0OKklJE029987-at-ns.microscopy.com} 14, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Link to current cryocourse June 6-15, 2006 at University of British Columbia, Vancouver, BC Here is the link to the current cryo course in 2006. http://www.emlab.ubc.ca/CryoEM2006/index.html
Judy Murphy wrote:
} The 5th International Cryo-EM Course is I believe June 6-17, 2006 in } Vancouver, Canada at University of British Columbia by Dr. Elaine } Humphrey from UBC and Dr. Kent McDonald, from UC, Berkeley with } international speakers. } This is an excellent course and caters to the needs of the particular } students. Be sure to check with Elaine about dates. } For more info email } Dr. Elaine Humphrey } ech-at-interchange.ubc.ca } } Information from the previous course is listed at } http://www.emlab.ubc.ca/CryoEM2005/index.html } } Judy } } } Judy Murphy, PhD } Microscopy Training, Imaging, and Lab Design } Stockton, CA 95219 } murphyjudy-at-comcast.net } } } } } } } } edelmare-at-muohio.edu wrote: } } } ---------------------------------------------------------------------------- } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } } } I have had a request from a user looking for a cryo- } } ultramicrotomy workshop. Is anyone offering one in the next year or } } know of one? Thanks! } } } } } } } } Richard E. Edelmann, Ph.D. } } Electron Microscopy Facility Director } } 364 Pearson Hall } } Miami University, Oxford, OH 45056 } } Ph: 513.529.5712 Fax: 513.529.4243 } } E-mail: edelmare-at-muohio.edu } } http://www.emf.muohio.edu } } } } "WE ARE MICROSOFT. } } RESISTANCE IS FUTILE. } } YOU WILL BE ASSIMILATED." } } } } ==============================Original } } Headers============================== } } 5, 23 -- From edelmare-at-muohio.edu Tue Jan 24 14:11:13 2006 } } 5, 23 -- Received: from mulnx12.mcs.muohio.edu } } (mulnx12.mcs.muohio.edu [134.53.6.67]) } } 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } } k0OKBBkX020625 } } 5, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 24 Jan 2006 } } 14:11:12 -0600 } } 5, 23 -- Received: from mulnx24.mcs.muohio.edu } } (mulnx24.mcs.muohio.edu [134.53.6.11]) } } 5, 23 -- by mulnx12.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) } } with ESMTP id k0OKB14j016596 } } 5, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 24 Jan 2006 } } 15:11:01 -0500 } } 5, 23 -- Received: from emf03 ([134.53.14.97]) } } 5, 23 -- by mulnx24.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) } } with ESMTP id k0OKB002022369 } } 5, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 24 Jan 2006 } } 15:11:00 -0500 } } 5, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} } } 5, 23 -- To: microscopy-at-Microscopy.com } } 5, 23 -- Date: Tue, 24 Jan 2006 15:11:49 -0500 } } 5, 23 -- MIME-Version: 1.0 } } 5, 23 -- Content-type: text/plain; charset=US-ASCII } } 5, 23 -- Content-transfer-encoding: 7BIT } } 5, 23 -- Subject: Cryo-ultramicrotomy workshop? } } 5, 23 -- Message-ID: {43D643B5.14764.643E209-at-localhost} } } 5, 23 -- Priority: normal } } 5, 23 -- X-mailer: Pegasus Mail for Win32 (v3.12c) } } 5, 23 -- X-Real-ConnectIP: 134.53.14.97 } } 5, 23 -- X-Scanned-By: MIMEDefang 2.45 } } 5, 23 -- X-Scanned-By: MIMEDefang 2.52 on 134.53.6.11 } } ==============================End of - } } Headers============================== } } } } } } } }
==============================Original Headers============================== 5, 19 -- From murphyjudy-at-comcast.net Wed Jan 25 09:57:45 2006 5, 19 -- Received: from sccrmhc14.comcast.net (sccrmhc14.comcast.net [204.127.202.59]) 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0PFviAM012953 5, 19 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jan 2006 09:57:44 -0600 5, 19 -- Received: from [192.168.1.102] (c-67-181-78-13.hsd1.ca.comcast.net[67.181.78.13]) 5, 19 -- by comcast.net (sccrmhc14) with ESMTP 5, 19 -- id {2006012515574101400ejlo9e} ; Wed, 25 Jan 2006 15:57:42 +0000 5, 19 -- Message-ID: {43D79FFF.4030800-at-comcast.net} 5, 19 -- Date: Wed, 25 Jan 2006 07:57:51 -0800 5, 19 -- From: Judy Murphy {murphyjudy-at-comcast.net} 5, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 5, 19 -- X-Accept-Language: en-us, en 5, 19 -- MIME-Version: 1.0 5, 19 -- To: Microscopy List Server {microscopy-at-microscopy.com} 5, 19 -- Subject: Re: [Microscopy] Cryo-ultramicrotomy workshop? 5, 19 -- References: {200601242046.k0OKklJE029987-at-ns.microscopy.com} {43D79E99.2010905-at-comcast.net} 5, 19 -- In-Reply-To: {43D79E99.2010905-at-comcast.net} 5, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I am in need of a manual for the Olympus BX41 microscope, particularly the epifluorescence unit, U-L100HGAPO. If anybody knows of on-line instructions, or how I could obtain a copy of the manuals, I would be very grateful. I will be happy to pay any copying and mailing charges, of course.
Ralph Common Dept. of Physiology Michigan State University
==============================Original Headers============================== 4, 24 -- From rcommon-at-msu.edu Wed Jan 25 10:07:41 2006 4, 24 -- Received: from sys18.mail.msu.edu (sys18.mail.msu.edu [35.9.75.118]) 4, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0PG7dLa015524 4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2006 10:07:39 -0600 4, 24 -- Received: from [35.9.122.125] (helo=emlab) 4, 24 -- by sys18.mail.msu.edu with esmtpsa (Exim 4.52 #1) 4, 24 -- (TLSv1:RC4-MD5:128) 4, 24 -- id 1F1nBK-0005iX-UD 4, 24 -- for Microscopy-at-microscopy.com; Wed, 25 Jan 2006 11:07:39 -0500 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: Olympus BX41 Manual 4, 24 -- Date: Wed, 25 Jan 2006 11:08:14 -0500 4, 24 -- Message-ID: {001201c621c9$8c9bed50$7d7a0923-at-msu.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="iso-8859-1" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 (Normal) 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1506 4, 24 -- Importance: Normal 4, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
Hello Everyone, Is there anyone out there who has experience etching Cu and Ni sulfides, in order to see compositional zoning ?
The phases I am interested in are: Cu2S and Ni3-XS2. In both these compounds Ni and Cu substitute for each other. It is these substitutions which I suspect change the etching behaviour of the compounds. Etching could thus be a quick and dirty alternative to excessive X-ray mapping/analysis.
I don't have the answer, but you may want to post this question on Metallography.com for wider exposure.
Stu Smalinskas, P.E. Metallurgist SKF Plymouth, Michigan
--- shem-at-laurentian.ca wrote: } } Hello Everyone, } Is there anyone out there who has experience etching } Cu and Ni sulfides, in order } to see compositional zoning ? } } The phases I am interested in are: Cu2S and Ni3-XS2. } In both these compounds Ni and Cu substitute } for each other. It is these substitutions which I } suspect change the etching behaviour of the } compounds. } Etching could thus be a quick and dirty alternative } to excessive X-ray mapping/analysis. } } Any input is welcome ! } } Thanks, } } Skage Hem } } } } _______________________________________________________________ } } Skage Hem } Research Scientist, Ph.D. } CAF, Department of Earth Sciences } Laurentian University } Ramsey Lake Road } Sudbury, ON } Canada } P3E 2C6 } } ph. 705-675-1151 x4040 } cell. 705-562-7321 } fax 705-675-4898 } } http://laurentian.ca/geology/Research/hem.html } }
__________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com
==============================Original Headers============================== 5, 20 -- From smalinskas-at-yahoo.com Wed Jan 25 15:39:12 2006 5, 20 -- Received: from web34105.mail.mud.yahoo.com (web34105.mail.mud.yahoo.com [66.163.178.103]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k0PLdBtD025507 5, 20 -- for {microscopy-at-sparc5.microscopy.com} ; Wed, 25 Jan 2006 15:39:11 -0600 5, 20 -- Received: (qmail 12361 invoked by uid 60001); 25 Jan 2006 21:39:10 -0000 5, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 20 -- s=s1024; d=yahoo.com; 5, 20 -- h=Message-ID:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 5, 20 -- b=1qmKDIUrud/YHnASQmqF6Vva92fi6hEC/jh1UNtRTvJH3OA4jU3Nb6FtrnQAiUR9lMgapzEfGIyQL+xNL6m1zL3xcbR+TJbBeW+komO12e06Smi5V2d6mx9x/L1/xO6qD4YjBOOzBjB4Ck7WEav53M6zgyoDWv+e+oZh+r9g318= ; 5, 20 -- Message-ID: {20060125213910.12359.qmail-at-web34105.mail.mud.yahoo.com} 5, 20 -- Received: from [141.151.33.213] by web34105.mail.mud.yahoo.com via HTTP; Wed, 25 Jan 2006 13:39:10 PST 5, 20 -- Date: Wed, 25 Jan 2006 13:39:10 -0800 (PST) 5, 20 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 5, 20 -- Subject: Re: [Microscopy] Etching of Cu-Ni matte for reflected light microscopy 5, 20 -- To: shem-at-laurentian.ca, 5, 20 -- "microscopy-at-sparc5.microscopy.com" {microscopy-at-ns.microscopy.com} 5, 20 -- In-Reply-To: {200601252114.k0PLE5YS017833-at-ns.microscopy.com} 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; charset=iso-8859-1 5, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
THE TEXAS SOCIETY FOR MICROSCOPY invites you to our Spring 2006 meeting on April 20-22, 2006 at Alcon Research Labs, 6201 South Freeway, Ft. Worth, TX 76134
All registration forms and lodging details are available on our web site: http://www.texasmicroscopy.org/
ABSTRACTS MUST BE RECEIVED BY: March 20, 2006 Advanced Registration Deadline: April 7, 2006. Advanced registration is strongly suggested to afford TSM an accurate participant count for event organization.
**Workshops— Thursday, April 20, 2006 (held at Alcon Labs, Ft. Worth)
“Microwave Immunology 101” Sponsored by Ted Pella, Inc. Speaker: Rick Giberson, Sr. Applications Engineer
“ESEM: not just for Biology Anymore” Sponsored by FEI Company Speaker: Daniel Phifer, Sr. Applications Engineer
**Guest Speaker — Friday, April 21, 2006
“Materials Science in Museums” Dr. Pamela Vandiver, Professor of Materials Science and Engineering and Archeology, Co-Director of Program in Heritage Conservation Science at the University of Arizona, and former Senior Research Scientist at the Smithsonian Institution’s Center for Materials Research and Education.
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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Invitation and Call for Papers Contamination Control in Electron and Ion Microscopy Microscopy and Microanalysis 2006, Chicago, IL Organizers: Ronald Vane (XEI Scientific) and András E. Vladár, Ph.D. (NIST) Session A17 (POSTERS ONLY) ABSTRACTS DUE February 15, 2006
This session is being organized to present the latest research on contamination sources and control techniques in charged beam microscopy. Herein we invite papers on all aspects of contamination control inside electron microscopes or focused ion beam tools including sources of contamination, the effects of contamination, contamination artifacts and interference on microscopy results, and contamination control methods and techniques. Contamination includes hydrocarbons, particulates, and other foreign matter that may interfere with imaging and analysis.
Traditionally, contamination control in SEMs has focused on pump oils, fingerprints, dirty specimens, and good vacuum practice in manufacturing and service. The use of dry pumps at all stages of the vacuum system of new FESEMs (Field Emission SEMs) and the use of better vacuum practices on the part on users and manufacturers have reduced contamination, but not eliminated problems. Most commonly, tools in the field begin to exhibit contamination artifacts after several months of use due to trace amounts of contaminants brought in with specimens. The Semiconductor and nano-sciences industries have demanded tools that can image structures {2 nm in size at { 2kV. Instrument manufacturers have responded with Field Emission tools that easily produce better than 400 kX magnification at high contrast with low kV beams. Control of contamination has assumed greater significance as semiconductor companies move to ever smaller dimensions. It is now common to observe features with {2 kV and {10 nm in size, close to these tools’ resolution limits. In such cases, the slightest amount of hydrocarbon (HC) contamination in the chamber can cause loss of resolution and contrast. The electron beam reacts with any stray HC in the beam path or on the surface creating HC ions that condense and form hydrocarbon deposits on the area being scanned. Even with baking, dry pumps and LN traps, artifacts and contamination haze remains.
What are the sources and what can be done? Some Research Topic Ideas:
* Do Hydrocarbons adsorbed on materials migrate on the surface to interact with charged beams to form deposits or is vapor phase transport more important? The effectiveness of cold fingers and Evactron(R) chamber cleaning suggests that vapor phase transport of contaminants is an important alternative mechanism to surface transport.
* Is the gas phase interaction of the electron beam and contaminant molecules important in SEM contamination buildup? Electron impact ionization is a common technique in mass spectrometry of organics. What is the importance of electron impact ionization on vapor phase organics in the SEM in causing contamination build up in scanned areas?
* What gives better results: plasma cleaning every specimen before introduction to the SEM chamber, Evactron chamber cleaning, or Evactron cleaning specimens in the chamber?
* Study Hydrocarbon removal rates for different decontamination methods, for different types of hydrocarbons and concentrations, under different Evactron De-Contaminator power and pressure settings, and for different pump speeds and flow rates during Evactron cleaning.
* How fast does AMC (atmospheric molecular contamination) accumulate on specimens in different environments after they are cleaned? Freshly prepared and cleaned specimens are known to be freer from contamination than specimens that have sat in room air for several days. Quantify this effect.
* Establish a standard procedure for depositing repeatable amounts of contamination for removal technique studies.
* Establish a protocol for measuring tool contamination that can be transferred between tools and used to compare tools or used to establish contamination standards or limits.
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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Email: kjl226-at-vt.edu Name: Kathy
Organization: Virginia Tech
Title-Subject: [Filtered] Embedding spores
Question: What is the best way to process and embed spores?
Why not just ask Olympus, I'm pretty sure they will have one as its still a current microscope. If you are happy with a photocopy expect to get it free of charge from your local rep (and I'm sure they will email you the pdf version as well).
Zeiss and Leica now have all the recent microscope manuals on-line, but I have obtained photocopied manuals for 20+ year old microscopes and fittings, and once even a BASIC program hardware/software guide for a very odd programmable HP+Zeiss motorised XY stage discontinued in the 1970's (in the 1990's). The most interesting bit was the BASIC 'Trace on' debugging command: 'TRON', but to be honest it worked better when Disney did it.
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
The session organizers for the Pharmaceutical Symposium of M&M 2006 are looking for additional speakers. Presentations should discuss biological or material science applications of significance to the pharmaceutical industry. The meeting will be held in Chicago, July 30 - Aug. 3. The abstract submission deadline is Feb. 15, 2006. If you are interested in submitting a platform or poster presentation, instructions for abstract submission are available at the meeting web site at http://mm2006.microscopy.org/ . For additional questions please contact the session chairs at the addresses below. Thanks.
Joe Neilly Abbott Laboratories 200 Abbott Park Rd. Bldg. AP31, Dept. R4R9 Abbott Park, IL 60064-6202 email: joe.neilly-at-abbott.com voice: 847-938-5024
Jim DiOreo Baxter Healthcare RL WG3-2S Route 120 and Wilson Road Round Lake, IL 60002 email: jim_dioreo-at-baxter.com voice: 847-270-4676
==============================Original Headers============================== 3, 18 -- From joe.p.neilly-at-abbott.com Thu Jan 26 07:59:45 2006 3, 18 -- Received: from abtmx5.abbott.com (abtmx5.abbott.com [130.36.44.95]) 3, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0QDxiEW028526 3, 18 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2006 07:59:45 -0600 3, 18 -- Received: from abtapn561.northamerica.intra.abbott.com (abtapn561.northamerica.intra.abbott.com [10.236.188.103]) 3, 18 -- by abtmx5.abbott.com (Switch-3.1.7/Switch-3.1.7) with ESMTP id k0QDxeU7010209 3, 18 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2006 07:59:40 -0600 (CST) 3, 18 -- To: microscopy-at-microscopy.com 3, 18 -- Subject: Request for Speakers at Pharmaceutical Symposium , M&M 2006 3, 18 -- X-Mailer: Lotus Notes Release 5.0.5 September 22, 2000 3, 18 -- Message-ID: {OFE0F856E6.F3ED62DE-ON86257102.004CC6F7-at-abbott.com} 3, 18 -- From: Joseph P Neilly {joe.p.neilly-at-abbott.com} 3, 18 -- Date: Thu, 26 Jan 2006 07:59:12 -0600 3, 18 -- X-MIMETrack: Serialize by Router on ABTAPN561/ESVR/ABBOTT(Release 6.5.4FP2|September 12, 2005) at 3, 18 -- 01/26/2006 07:59:42, 3, 18 -- Serialize complete at 01/26/2006 07:59:42 3, 18 -- MIME-Version: 1.0 3, 18 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
We have a networked color laser printer in our lab which we use to print routine image / working print from our SEM's and otehr microscopes. We print images and sectra, EBDS, etc. What we have found is that fewer and fewer folks actually want hardcopies. They want and use electronic versions, since even for publication nearly all of the journals these days want electronic only. My printer supplies are about 20% or lower of what they were 2-3 years ago (i.e. over an 80% decrease in printing - at the same time I've seen a 28% increase in user numbers).
For high quality and archive prints we use our Epson 9600 wide format printer - we generally use if for posters. I would not hesitate to get another Epson Pro series, perhaps in a smaller format for more routine size prints. Or look at the HP highend printers.
But remember the inks will dry in the printers if they are not regularly used. Some of the manufacturers have inks which are designed to last longer in the printer - but they are not the low- to medium end printers. Dry inks like laser printers and wax/polymer "phaser" printers have an advantage here.
FYI: We have a Lexmark C752 color laser printer. And whereas we've been very very happy with the durablity and quality of the Lexmark Black / Greyscale laser printers, we have not been thrilled with the image quality of the C752 prints, and yes we use the high quality color laser paper.
On 25 Jan 2006, at 7:44, fmalherbe-at-swin.edu.au wrote:
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (fmalherbe-at-swin.edu.au) from } http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html } on Tuesday, January 24, 2006 at 18:23:45 } ---------------------------------------------------------------------- } ----- } } Email: fmalherbe-at-swin.edu.au } Name: Francois Malherbe } } Organization: Swinburne University of Technology } } Education: Graduate College } } Location: Melbourne, Victoria, Australia } } Question: We are purchasing a new SEM, what are the basic specs you } would recommend for the printer: } } - inkjet, laser or other? } - USB, serial? } - dpi? } } Thank you for your help. } } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== 9, 12 -- From } zaluzec-at-ultra5.microscopy.com Wed Jan 25 07:33:17 2006 9, 12 -- } Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 9, } 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } k0PDXFin024204 9, 12 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jan } 2006 07:33:16 -0600 9, 12 -- Mime-Version: 1.0 9, 12 -- X-Sender: } zaluzec-at-ultra5.microscopy.com (Unverified) 9, 12 -- Message-Id: } {p06110405bffd2e8756cc-at-[206.69.208.22]} 9, 12 -- Date: Wed, 25 Jan } 2006 07:33:15 -0600 9, 12 -- To: microscopy-at-microscopy.com 9, 12 -- } From: fmalherbe-at-swin.edu.au (by way of Ask-A-Microscopist) 9, 12 -- } Subject: [Filtered] AskAMicroscopist: Printer for an SEM 9, 12 -- } Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"WE ARE MICROSOFT. RESISTANCE IS FUTILE. YOU WILL BE ASSIMILATED."
==============================Original Headers============================== 12, 25 -- From edelmare-at-muohio.edu Thu Jan 26 09:13:08 2006 12, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 12, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0QFD7wO013454 12, 25 -- for {microscopy-at-Microscopy.com} ; Thu, 26 Jan 2006 09:13:07 -0600 12, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 12, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id k0QFD5ac030704; 12, 25 -- Thu, 26 Jan 2006 10:13:05 -0500 12, 25 -- Received: from emf03 ([134.53.14.97]) 12, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id k0QFD4fg029547; 12, 25 -- Thu, 26 Jan 2006 10:13:04 -0500 12, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 12, 25 -- To: fmalherbe-at-swin.edu.au 12, 25 -- Date: Thu, 26 Jan 2006 10:13:59 -0500 12, 25 -- MIME-Version: 1.0 12, 25 -- Content-type: text/plain; charset=US-ASCII 12, 25 -- Content-transfer-encoding: 7BIT 12, 25 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: Printer for an SEM 12, 25 -- CC: microscopy-at-Microscopy.com 12, 25 -- Message-ID: {43D8A0E7.2878.F7FC50B-at-localhost} 12, 25 -- Priority: normal 12, 25 -- In-reply-to: {200601251344.k0PDiMAx027101-at-ns.microscopy.com} 12, 25 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 12, 25 -- X-Real-ConnectIP: 134.53.14.97 12, 25 -- X-Scanned-By: MIMEDefang 2.45 12, 25 -- X-Scanned-By: MIMEDefang 2.52 on 134.53.6.10 ==============================End of - Headers==============================
Do you mean using glass plates instead of film? If so ..... Plates are much thicker and heavier, these factors contribute to storage problems. Plates break when dropped, film does not. I know people who insist that plates give a better image but I have seen no evidence of that. The boxes that Kodak plates came in were great for storing lantern slides.
Geoff
Ken.Blight-at-cancer.org.uk wrote:
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-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 8, 32 -- From mcauliff-at-umdnj.edu Thu Jan 26 09:19:07 2006 8, 32 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126]) 8, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0QFJ71O014621 8, 32 -- for {microscopy-at-msa.microscopy.com} ; Thu, 26 Jan 2006 09:19:07 -0600 8, 32 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1]) 8, 32 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 263D14BE18 8, 32 -- for {microscopy-at-msa.microscopy.com} ; Thu, 26 Jan 2006 10:19:07 -0500 (EST) 8, 32 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 8, 32 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 785834BDF9 8, 32 -- for {microscopy-at-msa.microscopy.com} ; Thu, 26 Jan 2006 10:19:06 -0500 (EST) 8, 32 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 8, 32 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 8, 32 -- id {0ITP00L01GB01W-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 8, 32 -- for microscopy-at-msa.microscopy.com; Thu, 26 Jan 2006 10:19:06 -0500 (EST) 8, 32 -- Received: from [127.0.0.1] ([10.138.2.240]) 8, 32 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 8, 32 -- 2004)) with ESMTP id {0ITP00JRVGQKBF-at-Polaris.umdnj.edu} ; Thu, 8, 32 -- 26 Jan 2006 10:08:58 -0500 (EST) 8, 32 -- Date: Thu, 26 Jan 2006 10:08:10 -0500 8, 32 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 8, 32 -- Subject: Re: plates 8, 32 -- In-reply-to: {200601261358.k0QDwKjM028252-at-ns.microscopy.com} 8, 32 -- To: Ken.Blight-at-cancer.org.uk, 8, 32 -- MicroscopyListserver {microscopy-at-msa.microscopy.com} 8, 32 -- Message-id: {43D8E5DA.6030702-at-umdnj.edu} 8, 32 -- MIME-version: 1.0 8, 32 -- Content-type: text/plain; format=flowed; charset=us-ascii 8, 32 -- Content-transfer-encoding: 7BIT 8, 32 -- X-Accept-Language: en-us, en 8, 32 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 8, 32 -- Gecko/20040804 Netscape/7.2 (ax) 8, 32 -- References: {200601261358.k0QDwKjM028252-at-ns.microscopy.com} ==============================End of - Headers==============================
Sorry for the confusion caused -The imaging plates I am interested in are electron detection plates for high quality digital images. I have no other details otherwise I would not be asking the question!
ken
Ken Blight Senior Scientific Officer Electron Microscopy Cancer Research UK London England.
==============================Original Headers============================== 4, 14 -- From Ken.Blight-at-cancer.org.uk Thu Jan 26 09:29:15 2006 4, 14 -- Received: from magic.lif.icnet.uk (magic.lif.icnet.uk [143.65.100.6]) 4, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0QFTEVw017335 4, 14 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2006 09:29:14 -0600 4, 14 -- Received: from [143.65.59.12] ([143.65.59.12]) 4, 14 -- by magic.lif.icnet.uk (8.9.3p2/8.9.3) with ESMTP id PAA27526 4, 14 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2006 15:29:13 GMT 4, 14 -- Mime-Version: 1.0 4, 14 -- Message-Id: {a06200702bffe98674b18-at-[143.65.59.12]} 4, 14 -- Date: Thu, 26 Jan 2006 15:25:01 +0000 4, 14 -- To: microscopy-at-microscopy.com 4, 14 -- From: Ken Blight {Ken.Blight-at-cancer.org.uk} 4, 14 -- Subject: Imaging plates 4, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
What you want is described at http://www.berthold.com.au/imaging_pages/FDL-5000.html
and at http://www.ditabis.de/
I have no experience with use of these in EM. An impressive 3D cryo-EM paper based on use of the imaging plates was published by Holmes et al in Nature (2003), 425:423-427. Use of imaging plates requires drying the media before loading into the camera, and requires breaking camera vacuum and accessing the camera in order to remove media to "develop" (scan & digitize) the recorded images and regenerate the plate. Both steps are avoided when using CCD display and recording of EM images, which provide the other route for directly digitizing EM images without processing and scanning photographic negatives.
-mike reedy-
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-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
I hope you all will forgive this detour from microscopy. If you have a x-ray film processor in your lab and would care to comment on X-ray film processors I would love to hear from you off-list. We have an AFP Medical-Mini X-ray film processing system but we are looking into replacing it with a Konica SRX-101A desktop processor. Advice/ opinions on this equipment would be greatly appreciated. Thanks, Beth
********************************************************************** Beth Richardson EM Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
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Title-Subject: [Filtered] TEM - Seeking to acquire JEOL jem100c or equivalent.
Question: We are a small French company based in the Paris region, currently looking to replace our JEOL jem100c transmission electron microscope, which unfortunately, has broken down.
We need to acquire a second-hand microscope similar to the model we actually have but as we wish to use it mainly as an electron generator, to test the performance of the customized cameras we manufacture, we would accept a tem microscope, which does not offer all the requirements necessary for good quality imagery.
If you have such equipment and are willing to give us the opportunity of acquiring it, please contact us via our e-mail address: info-at-lheritier-sa.com.
It looks like we are losing the +24V section of the power supply on our Oxford ISIS EDS. Does anyone have a spare power supply, or even an entire ISIS unit they would be willing to part with? It probably should be a model 200 or 300.
Please contact me off-line and we can discuss the details.
Warren Straszheim Materials Analysis and Research Lab Iowa State University 515-294-8187
==============================Original Headers============================== 4, 29 -- From wesaia-at-iastate.edu Fri Jan 27 13:37:34 2006 4, 29 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15]) 4, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0RJbXOC001093 4, 29 -- for {Microscopy-at-Microscopy.Com} ; Fri, 27 Jan 2006 13:37:34 -0600 4, 29 -- Received: from mailout-2.iastate.edu (mailout-2.iastate.edu [129.186.140.2]) 4, 29 -- by mailhub-5.iastate.edu (8.12.11/8.12.10) with SMTP id k0RJbWHI009451 4, 29 -- for {Microscopy-at-Microscopy.Com} ; Fri, 27 Jan 2006 13:37:32 -0600 4, 29 -- Received: from owa.eng.iastate.edu(129.186.23.85) by mailout-2.iastate.edu via smtp 4, 29 -- id 7bca_5f49d450_8f6c_11da_9c60_003048290bef; 4, 29 -- Fri, 27 Jan 2006 13:37:37 -0600 4, 29 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.1830); 4, 29 -- Fri, 27 Jan 2006 13:37:32 -0600 4, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 4, 29 -- Content-class: urn:content-classes:message 4, 29 -- MIME-Version: 1.0 4, 29 -- Content-Type: text/plain; 4, 29 -- charset="us-ascii" 4, 29 -- Subject: Need ISIS power supply 4, 29 -- Date: Fri, 27 Jan 2006 13:38:47 -0600 4, 29 -- Message-ID: {16A330AC32056A40B32842EC4BB8D7275F1FFC-at-maire.eng.iastate.edu} 4, 29 -- X-MS-Has-Attach: 4, 29 -- X-MS-TNEF-Correlator: 4, 29 -- Thread-Topic: Need ISIS power supply 4, 29 -- Thread-Index: AcYjeUgRcQ/G34hJQ8agDRbdnqwxXA== 4, 29 -- From: "Straszheim, Warren E [CCE E]" {wesaia-at-iastate.edu} 4, 29 -- To: {Microscopy-at-Microscopy.Com} 4, 29 -- X-OriginalArrivalTime: 27 Jan 2006 19:37:33.0133 (UTC) FILETIME=[1E3347D0:01C62379] 4, 29 -- Content-Transfer-Encoding: 8bit 4, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0RJbXOC001093 ==============================End of - Headers==============================
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Question: Title: Advanced Techniques in Microscopy for Materials Characterization
Lecturers: David Joy, University of Tennessee Eric Lifshin, State University of New York George Vander Voort, Buehler Ltd. Raynald Gauvin, McGill University Brendan Griffin, University of Western Australia Rocco Cerchiara, Fischione Instruments Pierre Hovington, Hydro-Quebec Research Institute Tom Kelly, Imago Scientific Instruments Scott Sitzman, HKL Technology Marin Lagace, Hydro-Quebec Research Institute
When: May 8-12, 2006
Where: McGill University, Department of Mining, Metals and Materials Engineering M.H.Wong Building 3610 University Street Montreal, Quebec, Canada H3A 2B2
The worlds largest and most comprehensive microscopy and microanalysis meeting is coming to Chicago July 30-Aug 3, 2006. This is the perfect opportunity for both your and as well as undergraduate/graduate students to gain valuable insights to recent work by interacting with the worlds most prominent researchers, as well as learn about the latest techniques in the scientific symposia and in instrumentation.
In addition to more than 50 scientific sessions and symposia there will be a number of short courses/tutorials starting on the weekend and extending through the week.
The meeting also hosts the largest commerical exhibition of microscopy instrumentation at any venue.
This will be the perfect opportunity for you to take advantage of the premier international meeting happening in the USA as well as make valuable contacts for the future.
Of particular importance to students is the availability of scholarship, and other funding opportunities for students which will allow you to attend the meeting at reduced costs.
*Student receive reduced registration rates * Scholarships and Awards are available from the sponsoring societies * Poster awards are presented for the best student contributions * Student Bursaries (i.e. work part time during the meeting) can be used to help defray your expenses
A limited number of awards are also available for professional technical staff.
To be eligible for these opportunities you must submit a paper for either platform or poster presentation at the meeting. The deadline for submitting a paper to MM2006 is Feb. 15, 2006 (there are no extensions), which is only a few weeks away.
If you have any question please feel free to contact any of the Organizers, for detailed information please visit the meeting WWW site (http://mm2006.microscopy.org).
Again the paper submission deadline is Feb 15, 2006. I will also point out that the submission process is now entirely electronic so you still have plenty of time to prepare your work and submit it for review and consideration. All papers for accepted for the meeting are included in the proceedings which is published as supplement to the Journal of the Microscopy Society of America - Microscopy & Microanalysis.
Disclaimer: In additions to being your Friendly Neighborhood SysOp, I have the "honor" (cough) of being the Co-chair of the local arrangements committee for this meeting, thus I have a vested interest in getting you to come to Chicago!
Cheers...
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 16, 11 -- From zaluzec-at-microscopy.com Mon Jan 30 08:31:29 2006 16, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 16, 11 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0UEVSk1010268 16, 11 -- for {microscopy-at-microscopy.com} ; Mon, 30 Jan 2006 08:31:29 -0600 16, 11 -- Mime-Version: 1.0 16, 11 -- Message-Id: {p06110401c003ccb17092-at-[206.69.208.22]} 16, 11 -- Date: Mon, 30 Jan 2006 08:31:28 -0600 16, 11 -- To: microscopy-at-microscopy.com 16, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 16, 11 -- Subject: MM 2006 Paper Submission Deadline - Feb 15, 2006 16, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Hi Everyone I am looking for a EHT X-regulator for our old Phillips EM 300 TEM. Anybody knows a good place to buy this part or have such parts on sale please contact me at pjoshi-at-miami.edu Thank you
Pratik P. Joshi, Ph.D.
Assistant Scientist / Lab Manager Center for Advanced Microscopy Department of Chemistry, University of Miami 1301 Memorial Drive, Room 315 Miami, FL- 33146 Ph: (305)284-4736, Fax: (305)284-1880
==============================Original Headers============================== 2, 20 -- From pjoshi-at-miami.edu Mon Jan 30 08:58:40 2006 2, 20 -- Received: from EXCHANGE2.cgcent.miami.edu (ntexchsrv2.ir.miami.edu [129.171.32.59]) 2, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0UEwd88014815 2, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Jan 2006 08:58:39 -0600 2, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 20 -- Content-class: urn:content-classes:message 2, 20 -- MIME-Version: 1.0 2, 20 -- Content-Type: text/plain; 2, 20 -- charset="iso-8859-1" 2, 20 -- Subject: Re: Looking for EHT X Regulator unit for Phillips EM 300 TEM 2, 20 -- Date: Mon, 30 Jan 2006 09:58:34 -0500 2, 20 -- Message-ID: {B2BA593DA3EBE143A856ABA856D01F5D02DCFE55-at-EXCHANGE2.cgcent.miami.edu} 2, 20 -- X-MS-Has-Attach: 2, 20 -- X-MS-TNEF-Correlator: 2, 20 -- Thread-Topic: Looking for EHT X Regulator unit for Phillips EM 300 TEM 2, 20 -- Thread-Index: AcYlraSDK/Vfxr9oTDWLGaysH9W/UA== 2, 20 -- From: "Joshi, Pratik P" {pjoshi-at-miami.edu} 2, 20 -- To: {Microscopy-at-microscopy.com} 2, 20 -- Content-Transfer-Encoding: 8bit 2, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0UEwd88014815 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both brad.huggins-at-bp.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: brad.huggins-at-bp.com Name: Brad Huggins
Organization: BP Chemicals, Naperville, IL
Title-Subject: [Filtered] LM, Anyone with "Digital Microscope" familiarity/experience?
Question: (trouble with MS Outlook and spam errors, so I'm using this format)
For several remote, light microscope applications, I have been looking at the Keyence VHX-100, Digital Microscope. It looks to be a very capable and versatile tool. Aside from its portability, 3D imaging capability appears quite impressive to me. It looks capable of adding - very significantly - to the limited depth-of-focus imaging condition of our existing light microscope systems.
Does anyone on the ListServer have any experience (good, bad or otherwise) with this microscope? Or similar digital microscopes? If you would rather not respond online, please share with me off-line. I will share a summary of "anonymous responses" as appropriate.
Thanks in advance,
Brad Huggins
BP Chemicals Naperville, IL brad.huggins-at-bp.com 630 420-3668
I would like hear the comment on various sample preparation technique for confocal microscopy (indirect Immunofluorescence) which gives 1) best presarvation 2) minimum background noise
Is it advisible to dry the slide before switching over to 1 Ab and 2Ab. I mean to ask is this drying in between lead to some form of precipitate???
thanks in advance
Regards, Shrunali Kulkarni Scientist Institute of Microbial Technology India
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==============================Original Headers============================== 6, 18 -- From aarti_harle-at-yahoo.co.in Tue Jan 31 22:35:20 2006 6, 18 -- Received: from web8303.mail.in.yahoo.com (web8303.mail.in.yahoo.com [202.43.219.124]) 6, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k114ZGAT025969 6, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Jan 2006 22:35:17 -0600 6, 18 -- Received: (qmail 86863 invoked by uid 60001); 1 Feb 2006 04:35:18 -0000 6, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 18 -- s=s1024; d=yahoo.co.in; 6, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 6, 18 -- b=AkdqGUkec0jNq9kRo7Ys1VDUDL9lmWiPYTrbZimG2x1gKtvKw56/ZRooXkIC5egeuXa/ocAJv2H7KBo8JPcd2YKl7to7s51Bgw1hKsKk03kglfqBuZIch4AbZnTxASV5UcSCJ1oiDR9TfNDpAWfoQmdp+Ov+/Y2ZET4xGUPfwjE= ; 6, 18 -- Message-ID: {20060201043518.86861.qmail-at-web8303.mail.in.yahoo.com} 6, 18 -- Received: from [203.197.210.215] by web8303.mail.in.yahoo.com via HTTP; Tue, 31 Jan 2006 20:35:18 PST 6, 18 -- Date: Tue, 31 Jan 2006 20:35:18 -0800 (PST) 6, 18 -- From: Aarti Harle {aarti_harle-at-yahoo.co.in} 6, 18 -- Subject: Sample preparation technique for confocal microscopy 6, 18 -- To: Microscopy-at-microscopy.com 6, 18 -- MIME-Version: 1.0 6, 18 -- Content-Type: text/plain; charset=iso-8859-1 6, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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