Microscopy ListServer Archives  


File Requested = 0602.txt
Retrival Software Version=NJZ07060908

From: hagglundk1-at-nku.edu
Date: Wed, 1 Feb 2006 08:09:03 -0600
Subject: [Microscopy] ESEM chamber gas choices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Shrunali

Yes it works fine (as do many anti-fadents e.g Citifluor
http://www.citifluor.co.uk ), although we rarely get problems with DAPI
bleaching - I won't mention Hoescht as I can never spell it. Normally the
likes of Cy5 or TRITC type fluorochromes bleach first, and anyway DAPI is
normally only there in our case to identify the animal cells more easily
(and it really makes specimen focussing a cinch with our 'low contrast'
samples). Vectashield antifadents stop samples fading really quickly rather
than eliminating fading. So you still have to minimise laser (and Hg lamp)
exposure times (e.g. increase scan speed, reduce image size & image
averaging, increase gain, and, normally in the last resort, open up the
confocal iris), particularly if you are doing Z stacks (naturally time-lapse
won't be a problem with fixed 'Vectashield' samples).

Being Cell Biology, most of our specimens are derived from cell cultures and
so have little contrast, making cell visual separation and location more
difficult even with DIC. We only have a little 5 Mw 'violet' 405nm laser
with our Leica SP2 AOBS confocal, but it works very well with nuclear stains
despite being at the very edge of the DAPI/Hoescht excitation spectrum. We
mostly use Mattek dishes (Petri dishes with a hole at the base and little
cover slips glued on - see http://www.glass-bottom-dishes.com/ ) as all our
microscopes are inverted and we only use high power oil objectives. They
have the advantage that we can simply drip the Vectashield into the Petri
dish on top of the fixed cells and replenish if it dries out (samples often
keep for days or longer in a darkened fridge). With glass slides, the
coverslip needs sealing on over the Vectashield with nail varnish, but the
nail varnish (and marker pen ink) often dissolve into the immersion oil
making a bit of a mess, particularly with the inverted microscopes we use.
The nail varnish also sticks to the stage slide holder (as the slide has to
be placed upside down in inverted systems) making a sticky mess and knocking
the sample out of level.

With regard to preparation, we don't deal with microbiological specimens,
just mammalian cells - so I won't offer any advise. There are plenty of
recipes on the internet and in books though, and you can try contacting
staff at another microbiological institutes via on-line searches or
traditional 'old boy' networks. This list server tends to be biased a little
towards electron microscopy. You reduce confocal image 'noise' in samples by
lowering the scan speed, increasing image averaging and upping laser power
on the confocal - the exact opposite of that required to minimise beaching.
So there is always a compromise between image quality and fluorochrome
bleaching. If the sample preparation has worked well your sample would be
expected to be quite bright initially (prior to bleaching) - often problems
with dark images are due to experimental failure rather than the confocal
microscope and it's software.

Regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk



----- Original Message -----
X-from: {aarti_harle-at-yahoo.co.in}
To: {keith.morris-at-ucl.ac.uk}
Sent: Tuesday, January 31, 2006 1:02 PM

Dear Shrunali,

I forgot to add it (although I expect you have the link already) : The full
details on Vectorshield products [e.g. Hardset and Mounting Medium versions]
can be found on the manufacturers website :

http://www.vectorlabs.com/products.asp?catID=279&locID=609338

It naturally has the 'test on an inconspicuous area before using'
disclaimer. Anti-fadents have been reported to occasionally cause a leaching
of stain and loss of fluorescence in some instances.

Regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk


----- Original Message -----
X-from: {aarti_harle-at-yahoo.co.in}
To: {keith.morris-at-ucl.ac.uk}
Sent: Tuesday, January 31, 2006 1:02 PM

I am interested in hearing what type of gases people are using in their
ESEM applications. We recently learned our silicon drift detector is
incompatible with water vapor, and we apparently cannot complete any
x-ray microanalysis while working in standard ESEM or VP modes (I was
pretty surprised to learn this). We are thinking that using a dry gas
such as nitrogen or helium will allow us to work in ESEM modes and use
our x-ray system as well, as there is no potential for moisture
condensing on the crystal surface.

Our ESEM is a Quanta 200, and it is not equipped with the peltier stage,
so we mainly use the environmental modes to alleviate charging in
uncoated samples. The x-ray system was purchased with the microscope
about four years ago. I would rather not discuss the name of the x-ray
vendor on the list, as we have a significant investment in this detector
and do not foresee finding the money to purchase a new one soon.

Our first concern is the impact of the gas on spectra. We can coat
samples and run them in high vacuum mode, but customers like spectra
that represent the sample and not the coating material. We periodically
have samples that cannot be coated, so ESEM becomes critical for our
imaging needs. If the gas is going to have a dramatic impact on signal,
are we better off coating and running high vacuum?

Is there a best choice for chamber gas? Our service engineer recommends
nitrogen as having benefits for keeping the system clean. We can set it
up fairly quickly, and I have a spare tank and regulator. I have heard
of people using helium and believe that I read somewhere that there was
some advantage to using helium.

I am still trying to get information from the vendor on whether this
will allow us to use the x-ray and ESEM simultaneously, or whether we
have options with different collimators or windows. Their responses
have been pretty slow coming.



Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238


==============================Original Headers==============================
9, 23 -- From hagglundk1-at-nku.edu Wed Feb 1 08:09:02 2006
9, 23 -- Received: from mailfe2.nku.edu (mailfe2.nku.edu [192.122.237.68])
9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k11E92h6029071
9, 23 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 08:09:02 -0600
9, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211);
9, 23 -- Wed, 1 Feb 2006 09:09:02 -0500
9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0
9, 23 -- Content-class: urn:content-classes:message
9, 23 -- MIME-Version: 1.0
9, 23 -- Content-Type: text/plain;
9, 23 -- charset="us-ascii"
9, 23 -- Subject: ESEM chamber gas choices
9, 23 -- Date: Wed, 1 Feb 2006 09:09:01 -0500
9, 23 -- Message-ID: {F01E4499C4EC5842A8AB7198141AC72F35861A-at-mailfac1.hh.nku.edu}
9, 23 -- X-MS-Has-Attach:
9, 23 -- X-MS-TNEF-Correlator:
9, 23 -- Thread-Topic: ESEM chamber gas choices
9, 23 -- Thread-Index: AcYnOQywNZQdWqyLTzaT/PcS/myvmA==
9, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu}
9, 23 -- To: {microscopy-at-microscopy.com}
9, 23 -- X-OriginalArrivalTime: 01 Feb 2006 14:09:02.0302 (UTC) FILETIME=[0DB1E7E0:01C62739]
9, 23 -- Content-Transfer-Encoding: 8bit
9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k11E92h6029071
==============================End of - Headers==============================




From: lesley.bechtold-at-jax.org
Date: Wed, 1 Feb 2006 09:23:30 -0600
Subject: [Microscopy] Seeking information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I am looking for information about staffing, equipment and general practices in microscopy facilities at other institutions. If anyone else is interested in the same sort of information, please reply to me directly. Thank you.

Lesley Bechtold


Lesley S. Bechtold
Senior Manager, Histopathology & Microscopy Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322



==============================Original Headers==============================
7, 21 -- From lesley.bechtold-at-jax.org Wed Feb 1 09:23:28 2006
7, 21 -- Received: from carmen.jax.org (carmen.jax.org [192.43.249.16])
7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k11FNO0c004460
7, 21 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 09:23:26 -0600
7, 21 -- Received: from jcs-mid-prod.jax.org (jcs-mid-prod.jax.org [192.43.249.134])
7, 21 -- by carmen.jax.org (8.12.11/8.12.11) with ESMTP id k11FLEmh001364
7, 21 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 10:23:14 -0500 (EST)
7, 21 -- (envelope-from lesley.bechtold-at-jax.org)
7, 21 -- Received: from spikey.jax.org by jcs-mid-prod.jax.org
7, 21 -- with ESMTP id 65124051138807281; Wed, 01 Feb 2006 10:21:21 -0500
7, 21 -- From: "Lesley Bechtold" {lesley.bechtold-at-jax.org}
7, 21 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
7, 21 -- Subject: Seeking information
7, 21 -- Date: Wed, 1 Feb 2006 10:20:25 -0500
7, 21 -- Message-ID: {20060201102025314.00000001972-at-spikey}
7, 21 -- X-Mailer: Oracle Connector for Outlook 10.1.1.0.5 71011 (11.0.6568)
7, 21 -- X-Accept-Language: en-us, en
7, 21 -- MIME-Version: 1.0
7, 21 -- Content-Type: text/plain; charset=us-ascii
7, 21 -- Content-Transfer-Encoding: 8bit
7, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k11FNO0c004460
==============================End of - Headers==============================




From: ptomic-at-ciclonsemi.com
Date: Wed, 1 Feb 2006 10:00:46 -0600
Subject: [Microscopy] viaWWW: Electrolytical Metal Analysis Tool

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ptomic-at-ciclonsemi.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: ptomic-at-ciclonsemi.com
Name: Peter Tomic

Organization: Ciclon Semiconductor Device Corp.

Title-Subject: [Filtered] ELYMA

Question: I would like to hear from people in the semiconductor industry on their experience with ELYMAT [Electrolytical Metal Analysis Tool]. This is an imaging system for the analysis of metal and oxygen contamination in Si wafer technology.

Peter Tomic
Ciclon Semiconductor Device Corp.

---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Wed Feb 1 10:00:45 2006
7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k11G0gZ1012598
7, 12 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 10:00:43 -0600
7, 12 -- Mime-Version: 1.0
7, 12 -- X-Sender: (Unverified)
7, 12 -- Message-Id: {p06110401c0068b902ff3-at-[206.69.208.22]}
7, 12 -- Date: Wed, 1 Feb 2006 10:00:41 -0600
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: ptomic-at-ciclonsemi.com (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: Electrolytical Metal Analysis Tool
7, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: twigg-at-estd.nrl.navy.mil
Date: Wed, 1 Feb 2006 10:08:11 -0600
Subject: [Microscopy] viaWWW: Postdoctural Position at NRL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both twigg-at-estd.nrl.navy.mil as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: twigg-at-estd.nrl.navy.mil
Name: Mark Twigg

Organization: Naval Research Laboratory

Title-Subject: [Filtered] Postdoctural Position

Question: Postdoctoral Research Position
Electronics Science and Technology Division
Naval Research Laboratory, Washington, DC

We are seeking a Postdoctoral fellow with a strong transmission electron microscopy and extended-defects/nanostructures background to join our electronic materials program at NRL. You will utilize your skills to characterize SiC and GaN epitaxial layers, as well as nanoelectronic materials based on colloidal gold. We have our own Hitachi H9000UHR HRTEM and sample preparation laboratory. We also have access to a Philips CM30, a JEOL 2200 FETEM with Omega energy filter and Z-contrast STEM, and an FEI Nova 600 Dual Beam FIB. The qualified candidate should have a Ph.D. in Materials Science, Physics, Chemistry, or a related physical science or engineering discipline. Experience in a variety of electron microscopy techniques including HRTEM, CBED, and EDXS, and EELS is important. Experience with FIB, XTEM, and mechanical lapping sample preparation techniques is also desirable.
Participants must be citizens or permanent residents of the United States. Two postdoctoral study programs are available at the Naval Research Laboratory: NRC and ASEE. NRC postdoctoral positions are administered by the National Research Council, a division of the National Academy of Sciences. The ASEE Postdoctoral Fellowship Program is administered by the American Society for Engineering Education. Further information about NRL's NRC post-doc program is found on the NRC web site (http://hroffice.nrl.navy.mil/jobs/postdoc.htm).
Fellowships are awarded for one year and may be extended for a second year. The base annual stipend for both programs the first year is $62,886.

Point of Contact: Dr. Mark E. Twigg
Naval Research Laboratory
Code 6812
4555 Overlook Avenue, S.W.
Washington, DC 20375-5320

Email: twigg-at-estd.nrl.navy.mil
tel: (202) 404-8543
fax: (202) 404-7194


---------------------------------------------------------------------------

==============================Original Headers==============================
10, 12 -- From zaluzec-at-microscopy.com Wed Feb 1 10:08:10 2006
10, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k11G85cf014529
10, 12 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 10:08:08 -0600
10, 12 -- Mime-Version: 1.0
10, 12 -- X-Sender: (Unverified)
10, 12 -- Message-Id: {p06110404c0068d49973d-at-[206.69.208.22]}
10, 12 -- Date: Wed, 1 Feb 2006 10:08:05 -0600
10, 12 -- To: microscopy-at-microscopy.com
10, 12 -- From: twigg-at-estd.nrl.navy.mil (by way of MicroscopyListserver)
10, 12 -- Subject: viaWWW: Postdoctural Position at NRL
10, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: dyel-at-mail.nih.gov
Date: Wed, 1 Feb 2006 11:14:32 -0600
Subject: [Microscopy] viaWWW: Tyrodes-Cacodylate Buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dyel-at-mail.nih.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dyel-at-mail.nih.gov
Name: Chip Dye

Organization: NIH-NICHD

Title-Subject: [Filtered] Tyrodes-Cacodylate Buffer

Question: Dear ListServer:

Has anyone used this buffer before? I plan on using this to make up a fixative to perfuse fix a rat. I will be looking at myelin of the sciatic nerve. Why does this buffer have such a high osmolarity? Any thoughts?

Thank you!

Tyrodes-Cacodylate Buffer (mammalian Ringers) (401 mOsmols)
1 liter dd H2O
NaCl 6 gm
KCl 0.2 gm
Na(CH3)2AsO2.3H2O 10.7 gm
MgCl2.6H2O 0.1 gm
NaHCO3 1.0 gm
Glucose 1.0 gm
CaCl2.2H2 0.2 gm
(adjust to pH 7.4 with 3.1 %HNO3)


Chip Dye

Microscopist
Microscopy & Imaging Core
NICHD, NIH
Bldg. 49, Room 5W14
Bethesda, Maryland 20892-4480
Phone: 301-496-3627
FAX: 301-496-9939
Email: dyel-at-mail.nih.gov
Pager : Dial 102, 11568, your phone number
Outside campus: Dial 1-800-NIH-BEEP, 11568, you phone number
http://mic.nichd.nih.gov


---------------------------------------------------------------------------

==============================Original Headers==============================
13, 12 -- From zaluzec-at-microscopy.com Wed Feb 1 11:14:30 2006
13, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
13, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k11HERJC001032
13, 12 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 11:14:28 -0600
13, 12 -- Mime-Version: 1.0
13, 12 -- X-Sender: (Unverified)
13, 12 -- Message-Id: {p06110407c0069cdf3ea4-at-[206.69.208.22]}
13, 12 -- Date: Wed, 1 Feb 2006 11:14:27 -0600
13, 12 -- To: microscopy-at-microscopy.com
13, 12 -- From: dyel-at-mail.nih.gov (by way of MicroscopyListserver)
13, 12 -- Subject: viaWWW: Tyrodes-Cacodylate Buffer
13, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Wed, 1 Feb 2006 12:54:15 -0600
Subject: [Microscopy] Re: viaWWW: Tyrodes-Cacodylate Buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Chip,
I've never used Tyrode's in conjunction with cacodylate. Years ago,
when the lab I was in was collaborating with an electrophysiologist
looking at structure-function relationships in heart muscle, we used
Tyrode's because it is a bicarbonate-buffered solution and he could
keep the isolated papillary muscles happy in it for hours as long as
he bubbled oxygen-CO2 through it. Lovely structure (see various
papers by Robinson, TR, etal during the 1980's).
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175
http://www.cornellcelldevbiology.org

==============================Original Headers==============================
1, 21 -- From lcgould-at-med.cornell.edu Wed Feb 1 12:54:15 2006
1, 21 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45])
1, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k11IsEXQ002540
1, 21 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 12:54:15 -0600
1, 21 -- Received: from mpx1.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120])
1, 21 -- by smtp-gw2.med.cornell.edu (Switch-3.1.7/Switch-3.1.7) with ESMTP id k11IsBig011013
1, 21 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 13:54:11 -0500 (EST)
1, 21 -- Received: from [140.251.48.23] by mpx1.med.cornell.edu
1, 21 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005))
1, 21 -- with ESMTP id {0IU0000UJV6ABKC0-at-mpx1.med.cornell.edu} for
1, 21 -- microscopy-at-microscopy.com; Wed, 01 Feb 2006 13:54:11 -0500 (EST)
1, 21 -- Date: Wed, 01 Feb 2006 13:50:59 -0500
1, 21 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
1, 21 -- Subject: Re: [Microscopy] viaWWW: Tyrodes-Cacodylate Buffer
1, 21 -- In-reply-to: {200602011727.k11HRkBD005210-at-ns.microscopy.com}
1, 21 -- To: dyel-at-mail.nih.gov, microscopy-at-microscopy.com
1, 21 -- Message-id: {p06200705c006b2a8a260-at-[140.251.48.23]}
1, 21 -- MIME-version: 1.0
1, 21 -- Content-type: text/plain; charset=us-ascii; format=flowed
1, 21 -- References: {200602011727.k11HRkBD005210-at-ns.microscopy.com}
1, 21 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.1.0.0, Antispam-Data: 2006.02.01.100606
==============================End of - Headers==============================




From: dyel-at-mail.nih.gov
Date: Wed, 1 Feb 2006 13:03:24 -0600
Subject: [Microscopy] viaWWW: Tyrodes-Cacodylate Buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both dyel-at-mail.nih.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: dyel-at-mail.nih.gov
Name: Chip Dye

Organization: NIH-NICHD

Title-Subject: [Filtered] Tyrodes-Cacodylate Buffer

Question: Dear ListServer:

Has anyone used this buffer before? I plan on using this to make up a fixative to perfuse fix a rat. I will be looking at myelin of the sciatic nerve. Why does this buffer have such a high osmolarity? Any thoughts?

Thank you!

Tyrodes-Cacodylate Buffer (mammalian Ringers) (401 mOsmols)
1 liter dd H2O
NaCl 6 gm
KCl 0.2 gm
Na(CH3)2AsO2.3H2O 10.7 gm
MgCl2.6H2O 0.1 gm
NaHCO3 1.0 gm
Glucose 1.0 gm
CaCl2.2H2 0.2 gm
(adjust to pH 7.4 with 3.1 %HNO3)


Chip Dye

Microscopist
Microscopy & Imaging Core
NICHD, NIH
Bldg. 49, Room 5W14
Bethesda, Maryland 20892-4480
Phone: 301-496-3627
FAX: 301-496-9939
Email: dyel-at-mail.nih.gov
Pager : Dial 102, 11568, your phone number
Outside campus: Dial 1-800-NIH-BEEP, 11568, you phone number
http://mic.nichd.nih.gov


---------------------------------------------------------------------------

==============================Original Headers==============================
13, 12 -- From zaluzec-at-microscopy.com Wed Feb 1 13:03:23 2006
13, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
13, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k11HERJC001032
13, 12 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 11:14:28 -0600
13, 12 -- Mime-Version: 1.0
13, 12 -- X-Sender: (Unverified)
13, 12 -- Message-Id: {p06110407c0069cdf3ea4-at-[206.69.208.22]}
13, 12 -- Date: Wed, 1 Feb 2006 11:14:27 -0600
13, 12 -- To: microscopy-at-microscopy.com
13, 12 -- From: dyel-at-mail.nih.gov (by way of MicroscopyListserver)
13, 12 -- Subject: viaWWW: Tyrodes-Cacodylate Buffer
13, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: jae5-at-lehigh.edu
Date: Wed, 1 Feb 2006 15:13:05 -0600
Subject: [Microscopy] Free TEM Workshop Materials and Geology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Pan American Advanced Studies Institute
on
Transmission Electron Microscopy in Materials Science

July 9 to July 22, 2006

Expenses paid
Further information: http://www.pasi-tem2006.cl

At the University of Chile - in Santiago, Chile - a new field-emission
TEM is being installed. This microscope will form the basis of a Pan
American Advanced Studies Institute which will cover principles and
applications of TEM to topics in materials and geological sciences.
The Institute is funded by the National Science Foundation of the United
States.

We invite applications from students and young researchers who wish to
participate in this two-week intensive workshop. APPLICANTS WHO ARE
ACCEPTED WILL HAVE THEIR EXPENSES PAID. The number of participants in
the workshop will be limited to 48.

The lectures and laboratories (presented by a distinguished group of
lecturers) will treat a wide range of topics: principles and
applications of transmission electron microscopy; microanalysis; latest
results and advances; and sample preparation.

The Institute is open to participants from any country in the Americas
(including the U.S.A.). Participation by women and members of minority
groups is greatly encouraged. Details of how to apply are given on the
web site:
http://www.pasi-tem2006.cl
The closing date for applications is March 13, 2006

--
...........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


==============================Original Headers==============================
10, 22 -- From jae5-at-lehigh.edu Wed Feb 1 15:13:05 2006
10, 22 -- Received: from rain.CC.Lehigh.EDU (rain.CC.Lehigh.EDU [128.180.39.20])
10, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k11LD4QL023398
10, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 15:13:04 -0600
10, 22 -- Received: from [128.180.55.141] (Dyn055141.mat.Lehigh.EDU [128.180.55.141])
10, 22 -- (authenticated bits=0)
10, 22 -- by rain.CC.Lehigh.EDU (8.13.5/8.13.5) with ESMTP id k11LD3Wn022378
10, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
10, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 16:13:03 -0500
10, 22 -- Message-ID: {43E1245F.1070609-at-lehigh.edu}
10, 22 -- Date: Wed, 01 Feb 2006 16:13:03 -0500
10, 22 -- From: Alwyn Eades {jae5-at-lehigh.edu}
10, 22 -- Organization: Lehigh University
10, 22 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317)
10, 22 -- X-Accept-Language: en-us, en
10, 22 -- MIME-Version: 1.0
10, 22 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com}
10, 22 -- Subject: Free TEM Workshop Materials and Geology
10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
10, 22 -- Content-Transfer-Encoding: 7bit
10, 22 -- X-Virus-Scanned: ClamAV version 0.88, clamav-milter version 0.87 on rain.CC.Lehigh.EDU
10, 22 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 1 Feb 2006 20:27:17 -0600
Subject: [Microscopy] Re: ESEM chamber gas choices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The Quanta 200 uses a W filament. Unless it is a SFEG.
If there are no ion pumps, you should be able to use
Nitrogen or He. If there is an ion pump, do not use He.
It will kill the pump.

I found that for VP (20-120Pa) that N2 works fine and is
better than air (less vacuum issues). So, for ESEM, I would
think that N2 ought to be the gas of choice as well.

For VP mode, I do quant with EDAX Genesis using collected
values at two pressure/vacuum values. This is their VIP
option. I find good correlation between it and high vacuum
mode. Which EDS are you using? Does it have an ESEM
option mode?

gary g.




At 06:45 AM 2/1/2006, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
12, 20 -- From gary-at-gaugler.com Wed Feb 1 20:27:16 2006
12, 20 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
12, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k122RF4w004457
12, 20 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 20:27:16 -0600
12, 20 -- Received: (qmail 10416 invoked from network); 1 Feb 2006 18:27:15 -0800
12, 20 -- Received: by simscan 1.1.0 ppid: 10403, pid: 10405, t: 0.1857s
12, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
12, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211)
12, 20 -- by qsmtp2 with SMTP; 1 Feb 2006 18:27:14 -0800
12, 20 -- Message-Id: {6.2.3.4.2.20060201181844.0204ac20-at-mail.calweb.com}
12, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4
12, 20 -- Date: Wed, 01 Feb 2006 18:27:16 -0800
12, 20 -- To: hagglundk1-at-nku.edu
12, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
12, 20 -- Subject: Re: [Microscopy] ESEM chamber gas choices
12, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
12, 20 -- In-Reply-To: {200602011445.k11EjI9l000396-at-ns.microscopy.com}
12, 20 -- References: {200602011445.k11EjI9l000396-at-ns.microscopy.com}
12, 20 -- Mime-Version: 1.0
12, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: W.Muss-at-salk.at
Date: Thu, 2 Feb 2006 09:16:29 -0600
Subject: [Microscopy] Re: Tyrodes-Cacodylate Buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Good morning,
dear Chip,

it is a long time ago I have used a Tyrode-GA-mixture as a perfusion
fixative for rat brain (selective hypothalamic target preparation
containing ventricle III as well as magnocellular nuclear regions,
providing "open" blood vessels/capillaries in that region; followed by a
3-dim reconstruction of those nuclear regions)....

Later on I had to use also such perfusion mixtures for retrograde perfusion
of pig (renal) arteries......
I don't know or have at hand now the original receipt of } Tyrode {'s
Mammalian Ringer solution, but have found in my methods/techniques
collection } old { descriptions and protocols of the respective solutions and
measurement data.

I have seen that your mixing formula varies only in the amount of
Glucose......you indicate: 1 gm, in my descriptions I do have listed 10
grms.

On the other hand, when working with Tyrode's, initially I haven't mixed
the buffer with sodium cacodylate, but instead with a very small amount of
sodium-dihydrogenphosphate as follows:
NaCl: 6.0 gm
KCl: 0.2 gm
CaCl2 (as CaCl2.2H2O) 0.2 (0.25g) gm
MgCl2 (as MgCl2.6H2O) 0.1 (0.2. g) gm
sodium-dihydrogenphosphate 0.05 gm
Sodium-hydrogencarbonate(NaHCO3) 1.0 gm
Glucose 10.0 gm
------------------------------------------------------------------------
----
ad 1000 ml A.bidest (DD A.dest.)
Phosphate(s) must be dissolved extra and should finally be added when all
other } powder-substances { (! especially CaCl2 and MgCl2) are readily
dissolved.

I have noted in the protocol:
initial pH of the resulting solution: 8.4, approx. 275-277 mosmol
(measuring also pH 8.14, mosmol: 310, 290, 285, 282, 295, 295).

pH-correction with approx. 2.95 ml 1 N HCl to pH. 7.2

The fixative for perfusion consisted of:
160 ml 25% glutaraldehyde (which means a final GA-concentration of 4% in
the fixative) to be mixed with the above mentioned Tyrode stock solution to
an endvolume of 1000 ml.
If there formed a pale/ milky precipitate (which sometimes occured, perhaps
due to a poor GA-quality with a high amount of aldehyde polymers - we had
at that time in the late 1970ies - but most probably due to the
Na2HPO4/NaH2PO4 !) the solution should be / has to be filtrated.

After doing so, I noted: pH ==} 8.4, 545-550 mosmol

Since the pH of that fix-mixture increased (????) to 8.4 with time again,
it was necessary to adjust again with some ml of 1 N HCl to a pH of at
least 7.7 (the solution now seemed to act quite well as a } buffer { system !
) and finally,
having adjusted the solution to pH 7.2 again, I then measured 1160 /1190 /
1200 mosmol.

As the following washing buffer I used at that time a Tyrode solution as
given above, but added Glucose 25 g ad 1000 ml solution (which perhaps was
wrong), and measured (after pH correction with approx. 1.3 ml of 1 N HCl to
pH 7.2) 360 / 370 / 375 mosmol.

The 4% glutaraldehyde perhaps will contribute approx. 280 mosmol to the
total osmolarity (if calculating from some own measurements and some tables
found in the literaturefor 1, 1.5, 2.0 and 5 % GA-Solutions, respectively),
the rest up to 1200 mosmol therefore must originate from the (more
complete) dissociation of total ions dissolved in the tyrode's solution.

You state that the Tyrode solution you measured had 400/401 mosmol and is
thought to be unusually high.

I assume that there is a contribution by the sodium-cacodylate you added
(which is, if you use 10.7 g / 1000 ml, a final molarity of 0.05 mol) which
could be in the range of 100 mosmol.

Consider also that due to more effective dissociation of ionic components
in the solution the more diluted your fluid is, osmolarity will increase -
not in a 1:1 mode
(eg. Na-phosphate ions in Na2HPO4 and NaH2PO4 0,1 M will not have double
the osmol amount of 0.05 M, which in fact will be higher due to more
effective dissociation).

In my files I have other/additional data concerning the use of Tyrode's
ringer solution, I you like, I could share those with you on request
offline.

Best regards,

Wolfgang Muss
OR Dr. Wolfgang Muss
EM-Lab
====} with pride: 25 years in operation by 2nd of Feb. 2006 {====
Institute of Pathology, SALK
(Salzburger Landeskliniken gemeinnuetzige GesmbH)
Muellner Hauptstrasse 48
A-5020 SALZBURG AUSTRIA/EUROPE

and/or/alternatively

Paracelsus Medical Private University (PMU)
Institute of Pathology
Electron Microscopy Lab
Muellner Hauptstrasse 48
A-5020 SALZBURG, Austria/Europe
Phone work: +43+662+4482+4720
Mobile phone work:+43+662+4482-57704
Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o
W.Muss")
E-Mail work: W.Muss-at-SALK.at

Mobile-phone private: ++43+676+5 369-456
E-Mail private: wij.Muss-at-aon.at
------------------------------------------------------------------------
--------------------------------Ankuendigung namens der (Information on
behalf of)
Society for Cutaneous Ultrastructure Research (SCUR)
PLEASE VISIT THE UPDATED WEBSITE of SCUR at
} http://www.scur.org {
-------------------------------------------------------------------------
Forthcoming Meetings:
33rd Annual Meeting of the SCUR, 8th-10th June, 2006, WARSZAW, Poland
WEBSITE, containing all FORMS: http://www.scur.org.pl
Additional informations: send an E-Mail
kwoznia-at-amwaw.edu.pl

34th Annual Meeting of the SCUR, 11th-12th May, 2007, PRAGUE
Czech Republic

35th Annual Meeting of the SCUR, 11th -12th May, 2008, NARA or KYOTO, Japan
Joint Meeting with the
JSUCB, the Japanese Society for Ultrastructural Cutaneous Biology









----------
Von: dyel-at-mail.nih.gov[SMTP:dyel-at-mail.nih.gov]
Antwort an: dyel-at-mail.nih.gov
Gesendet: Mittwoch, 01. Februar 2006 20:07
An: W.Muss-at-salk.at
Betreff: [Microscopy] Tyrodes-Cacodylate Buffer

------------------------------------------------------------------------
----
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both dyel-at-mail.nih.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------
Email: dyel-at-mail.nih.gov
Name: Chip Dye
Organization: NIH-NICHD
Title-Subject: [Filtered] Tyrodes-Cacodylate Buffer

Question: Dear ListServer:

Has anyone used this buffer before? I plan on using this to make up a
fixative to perfuse fix a rat. I will be looking at myelin of the sciatic
nerve. Why does this buffer have such a high osmolarity? Any thoughts?

Thank you!

Tyrodes-Cacodylate Buffer (mammalian Ringers) (401 mOsmols)
1 liter dd H2O
NaCl 6.0 gm
KCl 0.2 gm
Na(CH3)2AsO2.3H2O 10.7 gm
MgCl2.6H2O 0.1 gm
NaHCO3 1.0 gm
Glucose 1.0 gm
CaCl2.2H2 0.2 gm
(adjust to pH 7.4 with 3.1 %HNO3)


Chip Dye

Microscopist
Microscopy & Imaging Core
NICHD, NIH
Bldg. 49, Room 5W14
Bethesda, Maryland 20892-4480
Phone: 301-496-3627
FAX: 301-496-9939
Email: dyel-at-mail.nih.gov
Pager : Dial 102, 11568, your phone number
Outside campus: Dial 1-800-NIH-BEEP, 11568, you phone number
http://mic.nichd.nih.gov


---------------------------------------------------------------------------

==============================Original
Headers==============================
13, 12 -- From zaluzec-at-microscopy.com Wed Feb 1 13:03:23 2006
13, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com
[206.69.208.22])
13, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
k11HERJC001032
13, 12 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 11:14:28 -0600
13, 12 -- Mime-Version: 1.0
13, 12 -- X-Sender: (Unverified)
13, 12 -- Message-Id: {p06110407c0069cdf3ea4-at-[206.69.208.22]}
13, 12 -- Date: Wed, 1 Feb 2006 11:14:27 -0600
13, 12 -- To: microscopy-at-microscopy.com
13, 12 -- From: dyel-at-mail.nih.gov (by way of MicroscopyListserver)
13, 12 -- Subject: viaWWW: Tyrodes-Cacodylate Buffer
13, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of -
Headers==============================



==============================Original Headers==============================
46, 28 -- From W.Muss-at-salk.at Thu Feb 2 09:16:29 2006
46, 28 -- Received: from Hermes.salk.at (hermes.lks.at [193.170.167.9])
46, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k12FGQZ7030117
46, 28 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2006 09:16:28 -0600
46, 28 -- Received: from localhost (localhost [127.0.0.1])
46, 28 -- by hermes.lks.at (Postfix) with ESMTP id A5CC05A9044;
46, 28 -- Thu, 2 Feb 2006 16:15:58 +0100 (CET)
46, 28 -- Received: from hermes.lks.at ([127.0.0.1])
46, 28 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024)
46, 28 -- with ESMTP id 38598-03; Thu, 2 Feb 2006 16:15:58 +0100 (CET)
46, 28 -- Received: from c1pa003 (unknown [192.168.42.3])
46, 28 -- by hermes.lks.at (Postfix) with SMTP id 408075A9041;
46, 28 -- Thu, 2 Feb 2006 16:15:58 +0100 (CET)
46, 28 -- Received: by localhost with Microsoft MAPI; Thu, 2 Feb 2006 16:15:56 +0100
46, 28 -- Message-ID: {01C62813.F27E7020.W.Muss-at-salk.at}
46, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at}
46, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at}
46, 28 -- To: "'dyel-at-mail.nih.gov'" {dyel-at-mail.nih.gov}
46, 28 -- Cc: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
46, 28 -- Subject: [Microscopy] Re: Tyrodes-Cacodylate Buffer
46, 28 -- Date: Thu, 2 Feb 2006 16:15:55 +0100
46, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at}
46, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor
46, 28 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211
46, 28 -- MIME-Version: 1.0
46, 28 -- Content-Type: text/plain; charset="us-ascii"
46, 28 -- Content-Transfer-Encoding: 7bit
46, 28 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at
==============================End of - Headers==============================




From: jae5-at-lehigh.edu
Date: Thu, 2 Feb 2006 09:36:47 -0600
Subject: [Microscopy] Re: ESEM chamber gas choices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Water in an ESEM affecting EDS detectors?

There are some things that are not clear about this problem. The
question was written as if the use of water in an ESEM is a problem
specifically for silicon drift detectors. Surely the problem is the
same for all types of detector; the problem is not with the detector but
with the window. In a system working correctly, the vacuum of the
detector is quite separate from the vacuum of the chamber. So it does
not matter what gas you use in the ESEM as long as the window is intact.

Does water in an ESEM cause the window of the detector to develop holes
more quickly than, say, nitrogen in the ESEM? This could be the case,
depending on the window design. We did have window failures in our
ESEM, but we are told that this was a temporary problem and that the
windows being sold now are just fine in an ESEM using water. Does
anyone have specific knowledge on this?

Alwyn Eades

--
...........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


==============================Original Headers==============================
6, 25 -- From jae5-at-lehigh.edu Thu Feb 2 09:36:46 2006
6, 25 -- Received: from rain.CC.Lehigh.EDU (rain.CC.Lehigh.EDU [128.180.39.20])
6, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k12FakDr007269
6, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Feb 2006 09:36:46 -0600
6, 25 -- Received: from [128.180.55.141] (Dyn055141.mat.Lehigh.EDU [128.180.55.141])
6, 25 -- (authenticated bits=0)
6, 25 -- by rain.CC.Lehigh.EDU (8.13.5/8.13.5) with ESMTP id k12Fakid004491
6, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT);
6, 25 -- Thu, 2 Feb 2006 10:36:46 -0500
6, 25 -- Message-ID: {43E2270E.4050600-at-lehigh.edu}
6, 25 -- Date: Thu, 02 Feb 2006 10:36:46 -0500
6, 25 -- From: Alwyn Eades {jae5-at-lehigh.edu}
6, 25 -- Organization: Lehigh University
6, 25 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317)
6, 25 -- X-Accept-Language: en-us, en
6, 25 -- MIME-Version: 1.0
6, 25 -- To: hagglundk1-at-nku.edu,
6, 25 -- "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com}
6, 25 -- Subject: Re: [Microscopy] ESEM chamber gas choices
6, 25 -- References: {200602011533.k11FXYuO006193-at-ns.microscopy.com}
6, 25 -- In-Reply-To: {200602011533.k11FXYuO006193-at-ns.microscopy.com}
6, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
6, 25 -- Content-Transfer-Encoding: 7bit
6, 25 -- X-Virus-Scanned: ClamAV version 0.88, clamav-milter version 0.87 on rain.CC.Lehigh.EDU
6, 25 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: phillipst-at-missouri.edu
Date: Thu, 2 Feb 2006 11:40:15 -0600
Subject: [Microscopy] Cytoviva system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone have experience with the Cytoviva imaging system. It looks to
me (from their website at www.cytovita.com like it is a retrofit
condenser. They claim a resolution better than 50 nm. In fact, their
website says "The typical optical microscope provides a maximum theoretical
resolution limit of 240nm. With resolution below 100nm and detection below
50nm, CytoViva allows you to see details never before possible with
traditional microscopy. " Can someone explain to me how one can improve on
the maximum theoretical resolution?

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



==============================Original Headers==============================
5, 18 -- From PhillipsT-at-missouri.edu Thu Feb 2 11:40:15 2006
5, 18 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49])
5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k12HeEAh018288
5, 18 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 2 Feb 2006 11:40:14 -0600
5, 18 -- Received: from um-exproto9.um.umsystem.edu ([207.160.151.148]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830);
5, 18 -- Thu, 2 Feb 2006 11:40:14 -0600
5, 18 -- Received: from phillips-dell.missouri.edu ([128.206.81.132]) by um-exproto9.um.umsystem.edu over TLS secured channel with Microsoft SMTPSVC(6.0.3790.1830);
5, 18 -- Thu, 2 Feb 2006 11:40:13 -0600
5, 18 -- Message-Id: {6.0.0.22.2.20060202113632.020227f8-at-pop.missouri.edu}
5, 18 -- X-Sender: phillipst-at-pop.missouri.edu
5, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 6.0.0.22
5, 18 -- Date: Thu, 02 Feb 2006 11:42:13 -0600
5, 18 -- To: Microscopy-at-msa.microscopy.com
5, 18 -- From: Tom Phillips {phillipst-at-missouri.edu}
5, 18 -- Subject: Cytoviva system
5, 18 -- Mime-Version: 1.0
5, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
5, 18 -- X-OriginalArrivalTime: 02 Feb 2006 17:40:13.0843 (UTC) FILETIME=[B8EF5E30:01C6281F]
==============================End of - Headers==============================




From: W.Muss-at-salk.at
Date: Thu, 2 Feb 2006 12:40:31 -0600
Subject: [Microscopy] Re: Cytoviva system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Prof Phillips,
perhaps the instrument of Cytoviva ( http://www.cytoviva.com ) is a further
development of a technique I found in PNAS 2002 (if you want I can provide
you with a pdf of this article). Unfortunately I have not followed that
thread.....

FYI: Title of work:

Fast 100-nm resolution three-dimensional microscope reveals structural
plasticity of mitochondria in live yeast
Alexander Egner, Stefan Jakobs, and Stefan W. Hell*

3370-3375 PNAS March 19, 2002 vol. 99 no. 6
By introducing beam-scanning multifocal multiphoton 4Pi-confocal
microscopy, we have attained fast fluorescence imaging of live
cells with axial super resolution. Rapid scanning of up to 64 pairs
of interfering high-angle fields and subsequent confocal detection
enabled us to perform three to five times finer optical sectioning
than confocal microscopy. In conjunction with nonlinear image
restoration, we demonstrate, to our knowledge for the first time,
three-dimensional imaging of live eukaryotic cells at an equilateral
resolution of ~ 100 nm. This imaging mode allowed us to reveal the
morphology and size of the green fluorescent protein-labeled
mitochondrial compartment of live Saccharomyces cerevisiae (bakers'
yeast) growing on different carbon sources. Our studies show
that mitochondria of cells grown on medium containing glycerol as
the only carbon source, as opposed to glucose-grown cells, exhibit
a strongly branched tubular reticulum. We determine the average
tubular diameter and find that it increases from 339 +/- 5 nm to
360 +/- 4 nm when changing from glucose to glycerol, that is, from
a fermentable to a nonfermentable carbon source. Moreover, this
change is associated with a 2.8-fold increase of the surface of the
reticulum, resulting in an average increase in volume of the
mitochondrial compartment by a factor of 3.0 +/- 0.2.
Best regards

Wolfgang Muss
Salzburg



----------
Von: phillipst-at-missouri.edu[SMTP:phillipst-at-missouri.edu]
Antwort an: phillipst-at-missouri.edu
Gesendet: Donnerstag, 02. Februar 2006 18:44
An: W.Muss-at-salk.at
Betreff: [Microscopy] Cytoviva system

------------------------------------------------------------------------
----
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Does anyone have experience with the Cytoviva imaging system. It looks to
me (from their website at www.cytovita.com like it is a retrofit
condenser. They claim a resolution better than 50 nm. In fact, their
website says "The typical optical microscope provides a maximum theoretical
resolution limit of 240nm. With resolution below 100nm and detection below
50nm, CytoViva allows you to see details never before possible with
traditional microscopy. " Can someone explain to me how one can improve on
the maximum theoretical resolution?

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



==============================Original
Headers==============================
5, 18 -- From PhillipsT-at-missouri.edu Thu Feb 2 11:40:15 2006
5, 18 -- Received: from um-exproto9.um.umsystem.edu
(um-exproto9.um.umsystem.edu [207.160.151.49])
5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
k12HeEAh018288
5, 18 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 2 Feb 2006 11:40:14
-0600
5, 18 -- Received: from um-exproto9.um.umsystem.edu ([207.160.151.148]) by
um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830);
5, 18 -- Thu, 2 Feb 2006 11:40:14 -0600
5, 18 -- Received: from phillips-dell.missouri.edu ([128.206.81.132]) by
um-exproto9.um.umsystem.edu over TLS secured channel with Microsoft
SMTPSVC(6.0.3790.1830);
5, 18 -- Thu, 2 Feb 2006 11:40:13 -0600
5, 18 -- Message-Id: {6.0.0.22.2.20060202113632.020227f8-at-pop.missouri.edu}
5, 18 -- X-Sender: phillipst-at-pop.missouri.edu
5, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 6.0.0.22
5, 18 -- Date: Thu, 02 Feb 2006 11:42:13 -0600
5, 18 -- To: Microscopy-at-msa.microscopy.com
5, 18 -- From: Tom Phillips {phillipst-at-missouri.edu}
5, 18 -- Subject: Cytoviva system
5, 18 -- Mime-Version: 1.0
5, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
5, 18 -- X-OriginalArrivalTime: 02 Feb 2006 17:40:13.0843 (UTC)
FILETIME=[B8EF5E30:01C6281F]
==============================End of -
Headers==============================



==============================Original Headers==============================
17, 28 -- From W.Muss-at-salk.at Thu Feb 2 12:40:31 2006
17, 28 -- Received: from Hermes.salk.at (hermes.lks.at [193.170.167.9])
17, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k12IeUdb028307
17, 28 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 2 Feb 2006 12:40:30 -0600
17, 28 -- Received: from localhost (localhost [127.0.0.1])
17, 28 -- by hermes.lks.at (Postfix) with ESMTP id 23EC55A9044;
17, 28 -- Thu, 2 Feb 2006 19:07:53 +0100 (CET)
17, 28 -- Received: from hermes.lks.at ([127.0.0.1])
17, 28 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024)
17, 28 -- with ESMTP id 46646-04; Thu, 2 Feb 2006 19:07:52 +0100 (CET)
17, 28 -- Received: from c1pa003 (unknown [192.168.42.3])
17, 28 -- by hermes.lks.at (Postfix) with SMTP id CAA6D5A9041;
17, 28 -- Thu, 2 Feb 2006 19:07:52 +0100 (CET)
17, 28 -- Received: by localhost with Microsoft MAPI; Thu, 2 Feb 2006 19:07:48 +0100
17, 28 -- Message-ID: {01C6282B.F53DA340.W.Muss-at-salk.at}
17, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at}
17, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at}
17, 28 -- To: "'phillipst-at-missouri.edu'" {phillipst-at-missouri.edu}
17, 28 -- Cc: "'Microscopy-at-msa.microscopy.com'" {Microscopy-at-msa.microscopy.com}
17, 28 -- Subject: [Microscopy] Re: Cytoviva system
17, 28 -- Date: Thu, 2 Feb 2006 19:07:47 +0100
17, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at}
17, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor
17, 28 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211
17, 28 -- MIME-Version: 1.0
17, 28 -- Content-Type: text/plain; charset="us-ascii"
17, 28 -- Content-Transfer-Encoding: 7bit
17, 28 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at
==============================End of - Headers==============================




From: larry-at-cymru.freewire.co.uk
Date: Thu, 2 Feb 2006 14:51:23 -0600
Subject: [Microscopy] Re: ESEM chamber gas choices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hi Alwyn,

My information on this is not definite but, my understanding is that
different EDS manufacturers use different process for producing their
detector windows. Consequently, there are different consequences
between detectors depending on the gases used in the chamber. I can't
see that there would be a difference between Si(Li) and SDD but I can
understand a difference between manufacturers.

Best regards,
--
Larry Stoter
JEOL (UK) Ltd
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com

PLEASE NOTE
1. Any mail other than plain text will be automatically deleted.
2. Any mail, legitimate or not, apparently or actually from hotmail,
netscape, yahoo or excite will automatically be deleted.
3. Mail with no subject or without a clear subject will be ignored :-)

==============================Original Headers==============================
5, 16 -- From larry-at-cymru.freewire.co.uk Thu Feb 2 14:51:22 2006
5, 16 -- Received: from get.freewire.net (get.freewire.net [195.184.229.250])
5, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k12KpLst006559
5, 16 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 2 Feb 2006 14:51:22 -0600
5, 16 -- Received: from [217.154.254.216] ([217.154.254.216])
5, 16 -- by get.freewire.net (8.11.6/8.11.6) with ESMTP id k12KpH004925;
5, 16 -- Thu, 2 Feb 2006 20:51:17 GMT
5, 16 -- Mime-Version: 1.0
5, 16 -- Message-Id: {p06210200c0081f8a361d-at-[217.154.254.125]}
5, 16 -- In-Reply-To: {200602021537.k12FbpsN009683-at-ns.microscopy.com}
5, 16 -- References: {200602021537.k12FbpsN009683-at-ns.microscopy.com}
5, 16 -- Date: Thu, 2 Feb 2006 20:48:41 +0000
5, 16 -- To: jae5-at-lehigh.edu, Microscopy-at-MSA.Microscopy.Com
5, 16 -- From: Larry Stoter {larry-at-cymru.freewire.co.uk}
5, 16 -- Subject: [Microscopy] Re: ESEM chamber gas choices
5, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: michael-at-shaffer.net
Date: Thu, 2 Feb 2006 16:31:55 -0600
Subject: [Microscopy] RE: Re: ESEM chamber gas choices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Larry writes ...

} My information on this is not definite but, my understanding is that
} different EDS manufacturers use different process for producing their
} detector windows. Consequently, there are different consequences
} between detectors depending on the gases used in the chamber. I can't
} see that there would be a difference between Si(Li) and SDD but I can
} understand a difference between manufacturers.

To bring this back to SDD, my understanding is that all SDD detector chips
are manufactured by KETEK ... While individual SDD detector manufacturers
will choose to employ different windows. I am still waiting to find out the
source of information regarding a general query about SDDs' incompatibility
with water vapor.

Genuinely, Michael Shaffer :o)

SEM/MLA Laboratory Coordinator
http://www.mun.ca/creait/maf/
Inco Innovation Centre
Memorial University
St. John's, Newfoundland



==============================Original Headers==============================
7, 21 -- From michael-at-shaffer.net Thu Feb 2 16:31:55 2006
7, 21 -- Received: from ws6-5.us4.outblaze.com (ws6-5.us4.outblaze.com [205.158.62.152])
7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k12MVtbM017649
7, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Feb 2006 16:31:55 -0600
7, 21 -- Received: (qmail 9845 invoked from network); 2 Feb 2006 22:31:55 -0000
7, 21 -- Received: from unknown (HELO rarewolf1) (michael-at-shaffer.net-at-205.251.84.119)
7, 21 -- by ws6-5.us4.outblaze.com with SMTP; 2 Feb 2006 22:31:54 -0000
7, 21 -- From: "michael shaffer" {michael-at-shaffer.net}
7, 21 -- To: {larry-at-cymru.freewire.co.uk} ,
7, 21 -- "MSA Microscopy list" {Microscopy-at-microscopy.com}
7, 21 -- Subject: RE: [Microscopy] Re: ESEM chamber gas choices
7, 21 -- Date: Thu, 2 Feb 2006 19:01:39 -0330
7, 21 -- Message-ID: {000201c62848$706e48f0$4f01a8c0-at-rarewolf1}
7, 21 -- MIME-Version: 1.0
7, 21 -- Content-Type: text/plain;
7, 21 -- charset="us-ascii"
7, 21 -- Content-Transfer-Encoding: 7bit
7, 21 -- X-Mailer: Microsoft Office Outlook 11
7, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
7, 21 -- Thread-Index: AcYoOqzcXnO24mvMRVios5iM13rJLgADMGFA
7, 21 -- In-Reply-To: {200602022052.k12KqIrL007363-at-ns.microscopy.com}
==============================End of - Headers==============================




From: Judith_A_Ruiz-at-whirlpool.com
Date: Thu, 2 Feb 2006 19:41:52 -0600
Subject: [Microscopy] viaWWW: lab service for hire, How much do you charge

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Judith_A_Ruiz-at-whirlpool.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Judith_A_Ruiz-at-whirlpool.com
Name: Judith Ruiz

Organization: Whirlpool corporation

Title-Subject: [Filtered] How much do you charge

Question: For those who do lab service for hire, how much is the
going rate for SEM/EDX work? I only do work within our corporation
but we're putting together a cost of what our work would cost if it
was done outside.

Any help would be appreciated.

---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Thu Feb 2 19:41:52 2006
7, 12 -- Received: from [192.168.1.27] (msdvpn21.msd.anl.gov [130.202.238.85])
7, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k131fo0l029994
7, 12 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2006 19:41:51 -0600
7, 12 -- Mime-Version: 1.0
7, 12 -- X-Sender: (Unverified)
7, 12 -- Message-Id: {p06020405c0086544914c-at-[192.168.1.27]}
7, 12 -- Date: Fri, 3 Feb 2006 12:41:48 +1100
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: Judith_A_Ruiz-at-whirlpool.com (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: lab service for hire, How much do you charge
7, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: mmstuason-at-yahoo.ca
Date: Thu, 2 Feb 2006 19:42:16 -0600
Subject: [Microscopy] viaWWW: type of CO2 do you use for CPD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mmstuason-at-yahoo.ca as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mmstuason-at-yahoo.ca
Name: Lizette Tuason

Title-Subject: [Filtered] Type of CO2 for CPD

Question: Hi everyone,

What type of CO2 do you use for CPD? The CO2 we
currently have hooked up to our Denton DCP-1 is
supercritical fluid extraction grade but it costs
more than CAD$750. I looked up some online sites
where people mentioned that they use just
standard CO2 (in the US). Iím not sure what
standard CO2 is and what the equivalent would be
here. Iím buying from Praxair (Canada) and there
are several categories that Iím not sure which
one I should be choosing.

Could anyone whoís doing CPD tell me what CO2
you buy ñ company and catalog number?

Thanks.

Lizette Tuason

---------------------------------------------------------------------------


==============================Original Headers==============================
10, 14 -- From zaluzec-at-microscopy.com Thu Feb 2 19:42:16 2006
10, 14 -- Received: from [192.168.1.27] (msdvpn21.msd.anl.gov [130.202.238.85])
10, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k131gCML030359
10, 14 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2006 19:42:15 -0600
10, 14 -- Mime-Version: 1.0
10, 14 -- X-Sender: (Unverified)
10, 14 -- Message-Id: {p06020406c008656498d2-at-[192.168.1.27]}
10, 14 -- Date: Fri, 3 Feb 2006 12:42:10 +1100
10, 14 -- To: microscopy-at-microscopy.com
10, 14 -- From: mmstuason-at-yahoo.ca (by way of MicroscopyListserver)
10, 14 -- Subject: viaWWW: type of CO2 do you use for CPD
10, 14 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
10, 14 -- Content-Transfer-Encoding: 8bit
10, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k131gCML030359
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Thu, 2 Feb 2006 20:20:13 -0600
Subject: [Microscopy] Re: viaWWW: lab service for hire, How much do you

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

$200/hour....$150/hour.... $125/hour...$100/hour....pick a number.

This is a nice set of numbers but there is more to outsourcing than just
the hourly rate...IMO. If a specimen is prepared in-house and then
sent out-house, what is the out-house person to look for? Well, you
will have to spend x hours writing a detailed procedural instruction
for what you want to be analyzed. If the specimen deviates from this,
then what? What is your writing time worth? Nothing?

The point is that there is great value in having the requester sitting
by the SEM operator. Look here, look there, what is this, what is that?
If you outsource, you will get exactly or less than what you ask for
since the operator does not know what to do outside of your written
request. If the job is so mundane (how many are?) then this is not
a problem. The SEM is a very valuable tool for many areas of interest.
But given a 12mm diameter specimen stub, which 2u are of the most interest?
"I'll know it when I see it." Right. but they threw the specimen over
the wall to the SEM folks. The results thrown back may not match up
with what was needed. The variability of specimens means that there is
no simple approach to evaluation. Plus, the requester is not at the
SEM to know that they saw what they needed the first time.

gary g.

Disclaimer: I do not do this sort of ambiguous work. However, I do
perform SEM analysis of unknown specimens and known specimens but I
know what to look for.


At 05:44 PM 2/2/2006, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
11, 21 -- From gary-at-gaugler.com Thu Feb 2 20:20:12 2006
11, 21 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
11, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k132KCki016497
11, 21 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2006 20:20:12 -0600
11, 21 -- Received: (qmail 387 invoked from network); 2 Feb 2006 18:20:12 -0800
11, 21 -- Received: by simscan 1.1.0 ppid: 384, pid: 385, t: 0.1682s
11, 21 -- scanners: regex: 1.1.0 attach: 1.1.0
11, 21 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211)
11, 21 -- by qsmtp2 with SMTP; 2 Feb 2006 18:20:12 -0800
11, 21 -- Message-Id: {6.2.3.4.2.20060202180159.02002198-at-mail.calweb.com}
11, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4
11, 21 -- Date: Thu, 02 Feb 2006 18:20:14 -0800
11, 21 -- To: Judith_A_Ruiz-at-whirlpool.com
11, 21 -- From: Gary Gaugler {gary-at-gaugler.com}
11, 21 -- Subject: Re: [Microscopy] viaWWW: lab service for hire, How much do you
11, 21 -- charge
11, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com}
11, 21 -- In-Reply-To: {200602030144.k131ioQN004044-at-ns.microscopy.com}
11, 21 -- References: {200602030144.k131ioQN004044-at-ns.microscopy.com}
11, 21 -- Mime-Version: 1.0
11, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: bfoster-at-mme1.com
Date: Fri, 3 Feb 2006 03:05:42 -0600
Subject: [Microscopy] Re: Cytoviva system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Tom,

You are partially right about CytoViva: it is a retrofittable condenser fitted with special optics and an illuminator system.

Re: Resolution:
I worked with Aetos at some length on the launch of CytoViva and have seen better than 90nm resolution (as measured with a Richardson test slide).

Re: how it works
Dr. Vitaly Vodyanoy, the inventor currently has a paper in progress which will explain the physics. My personal observations lead me to believe that there is some sort of resonance effect rather than the traditional scattering or diffraction we normally use for imaging. The result looks a lot like fluorescence (bright object/dark background), without the need for staining. It also has an interesting ability to optically section (much like DIC or confocal). We were able to watch spirochetes actually invading cells as well as see more detail in bacteria and some special marine cells.

I encourage you to visit their website, if only to see some interesting images. Their technical applications specialist, Dr. Tom Hasling, is usually quite happy to run samples and Byron Cheatham, their sales manager, can probably arrange for a demo in your lab, if you are serious about potential purchase.


The system compares extremely well to other systems on the market in the $150K range, at about 10% the cost. ... and there's no cost for looking!

Hope this was helpful,
Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text for the Spring semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.







At 11:42 AM 2/2/2006, phillipst-at-missouri.edu wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
22, 17 -- From bfoster-at-mme1.com Fri Feb 3 03:05:42 2006
22, 17 -- Received: from smtp109.sbc.mail.mud.yahoo.com (smtp109.sbc.mail.mud.yahoo.com [68.142.198.208])
22, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1395gKj030224
22, 17 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 03:05:42 -0600
22, 17 -- Received: (qmail 98384 invoked from network); 3 Feb 2006 09:05:41 -0000
22, 17 -- Received: from unknown (HELO barbsd505.mme1.com) (bfostermme-at-sbcglobal.net-at-68.94.37.39 with login)
22, 17 -- by smtp109.sbc.mail.mud.yahoo.com with SMTP; 3 Feb 2006 09:05:41 -0000
22, 17 -- Message-Id: {7.0.1.0.0.20060203025348.0204e158-at-mme1.com}
22, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0
22, 17 -- Date: Fri, 03 Feb 2006 03:05:43 -0600
22, 17 -- To: phillipst-at-missouri.edu, Microscopy Listserver {microscopy-at-microscopy.com}
22, 17 -- From: Barbara Foster {bfoster-at-mme1.com}
22, 17 -- Subject: Re: [Microscopy] Cytoviva system
22, 17 -- In-Reply-To: {200602021742.k12HggDM022539-at-ns.microscopy.com}
22, 17 -- References: {200602021742.k12HggDM022539-at-ns.microscopy.com}
22, 17 -- Mime-Version: 1.0
22, 17 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: bfoster-at-mme1.com
Date: Fri, 3 Feb 2006 03:15:06 -0600
Subject: [Microscopy] Re: viaWWW: LM, Anyone with "Digital Microscope"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Brad

I'd recommend you also look into the Hi-Scope from Hirox-USA. They provide some really interesting imaging modes and have been well accepted by a number of major customers here in the US.

Caveat: MME has no financial interest in this product.

Hope this helpful,

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text for the Spring semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.



At 08:32 PM 1/30/2006, brad.huggins-at-bp.com wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
15, 17 -- From bfoster-at-mme1.com Fri Feb 3 03:15:06 2006
15, 17 -- Received: from smtp107.sbc.mail.mud.yahoo.com (smtp107.sbc.mail.mud.yahoo.com [68.142.198.206])
15, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k139F6LN007226
15, 17 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 03:15:06 -0600
15, 17 -- Received: (qmail 40367 invoked from network); 3 Feb 2006 09:15:06 -0000
15, 17 -- Received: from unknown (HELO barbsd505.mme1.com) (bfostermme-at-sbcglobal.net-at-68.94.37.39 with login)
15, 17 -- by smtp107.sbc.mail.mud.yahoo.com with SMTP; 3 Feb 2006 09:15:06 -0000
15, 17 -- Message-Id: {7.0.1.0.0.20060201092440.01ff2348-at-mme1.com}
15, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0
15, 17 -- Date: Fri, 03 Feb 2006 03:15:08 -0600
15, 17 -- To: brad.huggins-at-bp.com, Microscopy Listserver {microscopy-at-microscopy.com}
15, 17 -- From: Barbara Foster {bfoster-at-mme1.com}
15, 17 -- Subject: Re: [Microscopy] viaWWW: LM, Anyone with "Digital Microscope"
15, 17 -- In-Reply-To: {200601310232.k0V2WUBj013521-at-ns.microscopy.com}
15, 17 -- References: {200601310232.k0V2WUBj013521-at-ns.microscopy.com}
15, 17 -- Mime-Version: 1.0
15, 17 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: M_Jarnik-at-fccc.edu
Date: Fri, 3 Feb 2006 08:51:46 -0600
Subject: [Microscopy] Re: viaWWW: type of CO2 do you use for CPD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Lizette,

You don't need the really pure stuff. We use food-grade, and in one trial found it cleaner (less water, oil, and particulates) than the expensive, "pure" siphon CO2.
Just be sure to use a siphon-CO2 (comes in a cylinder with a siphon tube, so you get liquid, not gas, coming out), and spend the money for filters and dehydrating sieves.

Phil


We have the same Denton you do and use something what Airgas calls "bone
dry", cat. No. CDBD200S with siphon tube. In addition, we use the
Tousimis liquid CO2 filter (cat. No. 8784, I believe it helps). We pay
about US$ 65 for a cylinder, if I remember well. This seems to be giving
good results for CPD of biological material (mostly mouse embryos) for SEM.

Hope this helps,

Michal

mmstuason-at-yahoo.ca wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
7, 21 -- From M_Jarnik-at-fccc.edu Fri Feb 3 08:51:46 2006
7, 21 -- Received: from azah.fccc.edu (azah.fccc.edu [131.249.4.237])
7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k13EpjM8030588
7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 08:51:46 -0600
7, 21 -- Received: from fccc.edu (emf4.fccc.edu [131.249.9.170])
7, 21 -- by azah.fccc.edu (8.12.11/8.12.11) with ESMTP id k13Epj1t020965
7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 09:51:45 -0500 (EST)
7, 21 -- Message-ID: {43E36E02.2040108-at-fccc.edu}
7, 21 -- Date: Fri, 03 Feb 2006 09:51:46 -0500
7, 21 -- From: Michal Jarnik {M_Jarnik-at-fccc.edu}
7, 21 -- Reply-To: M_Jarnik-at-fccc.edu
7, 21 -- Organization: Fox Chase Cancer Center
7, 21 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.4) Gecko/20030624 Netscape/7.1
7, 21 -- X-Accept-Language: en,cs
7, 21 -- MIME-Version: 1.0
7, 21 -- To: microscopy-at-microscopy.com
7, 21 -- Subject: Re: [Microscopy] viaWWW: type of CO2 do you use for CPD
7, 21 -- References: {200602030142.k131gpqb032061-at-ns.microscopy.com}
7, 21 -- In-Reply-To: {200602030142.k131gpqb032061-at-ns.microscopy.com}
7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 21 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: Jones_e1269-at-yahoo.com
Date: Fri, 3 Feb 2006 15:44:52 -0600
Subject: [Microscopy] viaWWW: Immuno EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Jones_e1269-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: Jones_e1269-at-yahoo.com
Name: Jason Jones

Organization: The University of Texas Health Science Center at San Antonio

Title-Subject: [Filtered] Immuno EM

Question:

I am looking for a facility to do immuno-EM on the yeast Candida
albicans for us. We're interested in intracellular localization of a
soluble secretory
protein, secreted aspartyl protease (Sap2p) in wild-type and a
secretory mutant strain we have generated.

We have polyclonal antibodies to Sap2p, which have worked quite well
in the published literature for immunoEM, and we have also 6X-His
tagged
this protein in our wild-type and mutant strains.

IWe do not have immunoEM available as a core facility here.

Thank you.

Sincerely,

Jason Jones

---------------------------------------------------------------------------

==============================Original Headers==============================
12, 12 -- From zaluzec-at-microscopy.com Fri Feb 3 15:44:52 2006
12, 12 -- Received: from [203.98.91.190] (msdvpn30.msd.anl.gov [130.202.238.94])
12, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k13LiUlI012886
12, 12 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 15:44:39 -0600
12, 12 -- Mime-Version: 1.0
12, 12 -- X-Sender: (Unverified)
12, 12 -- Message-Id: {p06020402c0097f13cbab-at-[203.98.91.126]}
12, 12 -- Date: Sat, 4 Feb 2006 08:43:59 +1100
12, 12 -- To: microscopy-at-microscopy.com
12, 12 -- From: Jones_e1269-at-yahoo.com (by way of MicroscopyListserver)
12, 12 -- Subject: viaWWW: Immuno EM
12, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: df-at-donnforbes.com
Date: Fri, 3 Feb 2006 15:45:13 -0600
Subject: [Microscopy] viaWWW: SEM for BWA Detection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both df-at-donnforbes.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: df-at-donnforbes.com
Name: Donn Forbes

Organization: Arasil, Inc.

Title-Subject: [Filtered] SEM for BWA Detection

Question: Why isn't SEM a standard technology for detecting and
identifying viruses and bacteria used as biological warfare agents?

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Fri Feb 3 15:45:13 2006
6, 12 -- Received: from [203.98.91.190] (msdvpn30.msd.anl.gov [130.202.238.94])
6, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k13LiwrP012969
6, 12 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 15:45:07 -0600
6, 12 -- Mime-Version: 1.0
6, 12 -- X-Sender: (Unverified)
6, 12 -- Message-Id: {p06020403c0097f29d0ba-at-[203.98.91.190]}
6, 12 -- Date: Sat, 4 Feb 2006 08:44:35 +1100
6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: df-at-donnforbes.com (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: SEM for BWA Detection
6, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: bcarrington-at-slfc.org
Date: Fri, 3 Feb 2006 15:48:45 -0600
Subject: [Microscopy] AskAMicroscopist: K-8 Grade Grammar School

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (bcarrington-at-slfc.org) from
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Friday, February 3, 2006 at 09:15:17
---------------------------------------------------------------------------

Email: bcarrington-at-slfc.org
Name: Brenda Carrington

Organization: The Learning Center

Education: K-8 Grade Grammar School

Location: Chesterfeild MO (St. Louis area)

Question: We are haing a microscope day on Feb. 27 from 10:00-3:00.
I don't know how to contact a local person to assist us.
We have over 100 kids grades 2-9.
We will be studying microbes and microscopes and using the GEMS materials.



---------------------------------------------------------------------------

==============================Original Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Fri Feb 3 15:48:44 2006
9, 12 -- Received: from [203.98.91.190] (msdvpn30.msd.anl.gov [130.202.238.94])
9, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k13LmUOv018992
9, 12 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 15:48:38 -0600
9, 12 -- Mime-Version: 1.0
9, 12 -- X-Sender: (Unverified)
9, 12 -- Message-Id: {p06020404c009800d064d-at-[203.98.91.190]}
9, 12 -- Date: Sat, 4 Feb 2006 08:48:27 +1100
9, 12 -- To: microscopy-at-microscopy.com
9, 12 -- From: bcarrington-at-slfc.org (by way of Ask-A-Microscopist)
9, 12 -- Subject: AskAMicroscopist: K-8 Grade Grammar School
9, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: dsoren-at-umich.edu
Date: Fri, 3 Feb 2006 15:48:58 -0600
Subject: [Microscopy] Cleaning TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,

We have inherited lots of copper TEM grids that are decades old.
Many of them have a dark patina on them, and, on those grids, we have
been losing our sections during alcohol-based uranyl acetate
staining. The sections remain when we use water-based uranyl
acetate, however. We have tried sonicating the grids in ethanol,
acetone, or chloroform, none of which has solved our problem.

Does anyone have any suggestions for cleaning them so that we will
not lose our sections during staining, or should we pitch them? We
really do prefer to stain with alcohol- based uranyl acetate, since
it provides a more intense staining.

As always, thanks for any suggestions you might have.

Dotty Sorenson

Dorothy Sorenson
Microscopy and Image-analysis Laboratory
Department of Cell and Developmental Biology
University Of Michigan Medical School
4643 Medical Science Building II
1301 Catherine
Ann Arbor, MI 48109-0616
(734)763-1170
FAX (734)763-1166


==============================Original Headers==============================
7, 17 -- From dsoren-at-umich.edu Fri Feb 3 15:48:58 2006
7, 17 -- Received: from pushingtin.mr.itd.umich.edu (pushingtin.mr.itd.umich.edu [141.211.14.78])
7, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k13LmwV1020156
7, 17 -- for {microscopy-at-msa.microscopy.com} ; Fri, 3 Feb 2006 15:48:58 -0600
7, 17 -- Received: from [141.214.170.47] (host-47.subnet-170.med.umich.edu [141.214.170.47])
7, 17 -- by pushingtin.mr.itd.umich.edu (smtp) with ESMTP id k13LmvJZ004383;
7, 17 -- Fri, 3 Feb 2006 16:48:57 -0500
7, 17 -- Mime-Version: 1.0 (Apple Message framework v746.2)
7, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
7, 17 -- Message-Id: {F847072A-3C48-4995-B594-5900360673CB-at-umich.edu}
7, 17 -- Cc: Sasha Meshinchi {meshin-at-umich.edu}
7, 17 -- Content-Transfer-Encoding: 7bit
7, 17 -- From: Dorothy Sorenson {dsoren-at-umich.edu}
7, 17 -- Subject: Cleaning TEM grids
7, 17 -- Date: Fri, 3 Feb 2006 16:47:15 -0500
7, 17 -- To: microscopy-at-msa.microscopy.com
7, 17 -- X-Mailer: Apple Mail (2.746.2)
==============================End of - Headers==============================




From: GBurgess-at-exchange.hsc.mb.ca
Date: Fri, 3 Feb 2006 16:08:11 -0600
Subject: [Microscopy] RE: Cleaning TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Dotty,
I would try putting your grids into a drying oven for at least 5 minutes
after you cut your ultrathin sections. After they are totally dried down,
you can take them out. If you forget and leave your grids in the oven
overnight, it doesn't seem to hurt anything at all. The heat seems to bond
the sections to the grids very well, such that I've never had an experience
of sections coming off the grids, regardless of the state of the grids.

To clean my grids, I just dip them into concentrated sodium hydroxide for a
few seconds, and then rinse them by dipping them a few times in distilled
water just before I pick up the sections. It sort of etches the grids.

Garry Burgess
Charge Technologist - Electron Microscopy
Health Science Centre
Winnipeg, Canada



Dear listers,

We have inherited lots of copper TEM grids that are decades old.
Many of them have a dark patina on them, and, on those grids, we have
been losing our sections during alcohol-based uranyl acetate
staining. The sections remain when we use water-based uranyl
acetate, however. We have tried sonicating the grids in ethanol,
acetone, or chloroform, none of which has solved our problem.

Does anyone have any suggestions for cleaning them so that we will
not lose our sections during staining, or should we pitch them? We
really do prefer to stain with alcohol- based uranyl acetate, since
it provides a more intense staining.

As always, thanks for any suggestions you might have.

Dotty Sorenson

Dorothy Sorenson
Microscopy and Image-analysis Laboratory
Department of Cell and Developmental Biology
University Of Michigan Medical School
4643 Medical Science Building II
1301 Catherine
Ann Arbor, MI 48109-0616
(734)763-1170
FAX (734)763-1166


==============================Original Headers==============================
7, 17 -- From dsoren-at-umich.edu Fri Feb 3 15:48:58 2006
7, 17 -- Received: from pushingtin.mr.itd.umich.edu
(pushingtin.mr.itd.umich.edu [141.211.14.78])
7, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
k13LmwV1020156
7, 17 -- for {microscopy-at-msa.microscopy.com} ; Fri, 3 Feb 2006
15:48:58 -0600
7, 17 -- Received: from [141.214.170.47] (host-47.subnet-170.med.umich.edu
[141.214.170.47])
7, 17 -- by pushingtin.mr.itd.umich.edu (smtp) with ESMTP id
k13LmvJZ004383;
7, 17 -- Fri, 3 Feb 2006 16:48:57 -0500
7, 17 -- Mime-Version: 1.0 (Apple Message framework v746.2)
7, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes;
format=flowed
7, 17 -- Message-Id: {F847072A-3C48-4995-B594-5900360673CB-at-umich.edu}
7, 17 -- Cc: Sasha Meshinchi {meshin-at-umich.edu}
7, 17 -- Content-Transfer-Encoding: 7bit
7, 17 -- From: Dorothy Sorenson {dsoren-at-umich.edu}
7, 17 -- Subject: Cleaning TEM grids
7, 17 -- Date: Fri, 3 Feb 2006 16:47:15 -0500
7, 17 -- To: microscopy-at-msa.microscopy.com
7, 17 -- X-Mailer: Apple Mail (2.746.2)
==============================End of - Headers==============================

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

==============================Original Headers==============================
14, 20 -- From GBurgess-at-exchange.hsc.mb.ca Fri Feb 3 16:08:11 2006
14, 20 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca [142.233.100.122])
14, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k13M8A8U017896
14, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 16:08:11 -0600
14, 20 -- Received: from hscxntmx0007.hsc.mb.ca (unverified [172.16.6.179]) by
14, 20 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS 4.0.346.0) with ESMTP id
14, 20 -- {B0017955251-at-hscxntmx0003.hsc.mb.ca} for {Microscopy-at-microscopy.com} ;Fri,
14, 20 -- 3 Feb 2006 16:08:04 -0600
14, 20 -- Received: by hscxntmx0007.hsc.mb.ca with Internet Mail Service
14, 20 -- (5.5.2653.19)id {DXXMZXB1} ; Fri, 3 Feb 2006 16:08:43 -0600
14, 20 -- Message-ID:
14, 20 -- {00A937989100304A83A058F6C45873FF32A393-at-hscxntmx0005.hsc.mb.ca}
14, 20 -- Date: Fri, 3 Feb 2006 16:04:40 -0600
14, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca}
14, 20 -- Subject: RE: [Microscopy] Cleaning TEM grids
14, 20 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
14, 20 -- X-Mailer: Internet Mail Service (5.5.2653.19)
14, 20 -- MIME-Version: 1.0
14, 20 -- Content-Type: text/plain;
14, 20 -- charset="iso-8859-1"
==============================End of - Headers==============================




From: gstrout-at-ou.edu
Date: Fri, 3 Feb 2006 17:33:44 -0600
Subject: [Microscopy] Re: Cleaning TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We clean our grids in acid alcohol, a mixture of 3% HCL in 95% ethanol.
Cover the grids in a small shell vial with the acid alcohol, swirl
intermittantly for 30 to 40 seconds or so and rinse well with 95%
ethanol. If the grids have a very dark patina then clean for a longer
time. You'll know when they are done because they'll have a shiny,
bright copper finish.
We usually clean many grids at a time, but you could clean them one by
one by dipping the grid in the acid alcohol and then rinsing in a stream
of 95% ETOH just before you use them. This works better on grids that
have been cleaned, but have set around for a while, long enough to have
developed a small oxidized layer. Dark patinas like the grids you have
will probably need to be put into a vial and cleaned as described above.

dsoren-at-umich.edu wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

--
--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================



==============================Original Headers==============================
6, 21 -- From gstrout-at-ou.edu Fri Feb 3 17:33:43 2006
6, 21 -- Received: from c3p0.ou.edu (mail.zero.ou.edu [129.15.0.75])
6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k13NXhBV028012
6, 21 -- for {Microscopy-at-Sparc5.Microscopy.Com} ; Fri, 3 Feb 2006 17:33:43 -0600
6, 21 -- Received: from [127.0.0.1] ([129.15.38.202])
6, 21 -- by c3p0.ou.edu (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28
6, 21 -- 2005)) with ESMTP id {0IU400EHDXG5Q5A0-at-c3p0.ou.edu} for
6, 21 -- Microscopy-at-Sparc5.Microscopy.Com; Fri, 03 Feb 2006 17:33:41 -0600 (CST)
6, 21 -- Date: Fri, 03 Feb 2006 17:33:40 -0600
6, 21 -- From: Greg Strout {gstrout-at-ou.edu}
6, 21 -- Subject: Re: [Microscopy] Cleaning TEM grids
6, 21 -- In-reply-to: {200602032150.k13LoAHd025474-at-ns.microscopy.com}
6, 21 -- To: Microscopy List Server {Microscopy-at-ns.Microscopy.Com} , dsoren-at-umich.edu
6, 21 -- Message-id: {43E3E854.7040107-at-ou.edu}
6, 21 -- MIME-version: 1.0
6, 21 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed
6, 21 -- Content-transfer-encoding: 7BIT
6, 21 -- X-Accept-Language: en-us, en
6, 21 -- References: {200602032150.k13LoAHd025474-at-ns.microscopy.com}
6, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2)
6, 21 -- Gecko/20040804 Netscape/7.2 (ax)
==============================End of - Headers==============================




From: walck-at-southbaytech.com
Date: Fri, 3 Feb 2006 20:16:09 -0600
Subject: [Microscopy] Cleaning TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Try soaking a few grids in store grade ammonia for about 1/2 hr to 1 hr
to see if the patina is removed at all. Rinse well in distilled water
and see what happens.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: dsoren-at-umich.edu [mailto:dsoren-at-umich.edu]
Sent: Friday, February 03, 2006 1:53 PM
To: Walck-at-SouthBayTech.com

Dear listers,

We have inherited lots of copper TEM grids that are decades old.
Many of them have a dark patina on them, and, on those grids, we have
been losing our sections during alcohol-based uranyl acetate
staining. The sections remain when we use water-based uranyl
acetate, however. We have tried sonicating the grids in ethanol,
acetone, or chloroform, none of which has solved our problem.

Does anyone have any suggestions for cleaning them so that we will
not lose our sections during staining, or should we pitch them? We
really do prefer to stain with alcohol- based uranyl acetate, since
it provides a more intense staining.

As always, thanks for any suggestions you might have.

Dotty Sorenson

Dorothy Sorenson
Microscopy and Image-analysis Laboratory
Department of Cell and Developmental Biology
University Of Michigan Medical School
4643 Medical Science Building II
1301 Catherine
Ann Arbor, MI 48109-0616
(734)763-1170
FAX (734)763-1166


==============================Original
Headers==============================
7, 17 -- From dsoren-at-umich.edu Fri Feb 3 15:48:58 2006
7, 17 -- Received: from pushingtin.mr.itd.umich.edu
(pushingtin.mr.itd.umich.edu [141.211.14.78])
7, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
k13LmwV1020156
7, 17 -- for {microscopy-at-msa.microscopy.com} ; Fri, 3 Feb 2006
15:48:58 -0600
7, 17 -- Received: from [141.214.170.47]
(host-47.subnet-170.med.umich.edu [141.214.170.47])
7, 17 -- by pushingtin.mr.itd.umich.edu (smtp) with ESMTP id
k13LmvJZ004383;
7, 17 -- Fri, 3 Feb 2006 16:48:57 -0500
7, 17 -- Mime-Version: 1.0 (Apple Message framework v746.2)
7, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes;
format=flowed 7, 17 -- Message-Id:
{F847072A-3C48-4995-B594-5900360673CB-at-umich.edu}
7, 17 -- Cc: Sasha Meshinchi {meshin-at-umich.edu}
7, 17 -- Content-Transfer-Encoding: 7bit
7, 17 -- From: Dorothy Sorenson {dsoren-at-umich.edu}
7, 17 -- Subject: Cleaning TEM grids
7, 17 -- Date: Fri, 3 Feb 2006 16:47:15 -0500
7, 17 -- To: microscopy-at-msa.microscopy.com
7, 17 -- X-Mailer: Apple Mail (2.746.2)
==============================End of -
Headers==============================


==============================Original Headers==============================
17, 27 -- From walck-at-southbaytech.com Fri Feb 3 20:16:08 2006
17, 27 -- Received: from ylpvm15.prodigy.net (ylpvm15-ext.prodigy.net [207.115.57.46])
17, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k142G7Bg007081
17, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 20:16:08 -0600
17, 27 -- Received: from pimout7-ext.prodigy.net (pimout7-int.prodigy.net [207.115.4.147])
17, 27 -- by ylpvm15.prodigy.net (8.12.10 outbound/8.12.10) with ESMTP id k142GKEV029956
17, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 21:16:20 -0500
17, 27 -- X-ORBL: [64.169.193.90]
17, 27 -- Received: from dynamicbl8uno3 (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90])
17, 27 -- by pimout7-ext.prodigy.net (8.13.4 outbound domainkey aix/8.13.4) with ESMTP id k142Fvmf095186;
17, 27 -- Fri, 3 Feb 2006 21:16:02 -0500
17, 27 -- From: "Scott Walck" {walck-at-southbaytech.com}
17, 27 -- To: {dsoren-at-umich.edu}
17, 27 -- Cc: {Microscopy-at-microscopy.com}
17, 27 -- Subject: RE: [Microscopy] Cleaning TEM grids
17, 27 -- Date: Fri, 3 Feb 2006 18:16:01 -0800
17, 27 -- Message-ID: {007801c62930$f81347e0$7801a8c0-at-dynamicbl8uno3}
17, 27 -- MIME-Version: 1.0
17, 27 -- Content-Type: text/plain;
17, 27 -- charset="us-ascii"
17, 27 -- Content-Transfer-Encoding: 7bit
17, 27 -- X-Priority: 3 (Normal)
17, 27 -- X-MSMail-Priority: Normal
17, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.2627
17, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
17, 27 -- In-Reply-To: {200602032153.k13LrLc5007111-at-ns.microscopy.com}
17, 27 -- Importance: Normal
==============================End of - Headers==============================




From: dianavd-at-eye.usyd.edu.au
Date: Sun, 5 Feb 2006 22:06:54 -0600
Subject: [Microscopy] Re: Cleaning TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I've always cleaned grids by quickly passing through the flame of an
alcohol burner. Quick and easy. Works on my old grids, though they
aren't decades old!


Diana van Driel
Dept Ophthalmology
Sydney University
GPO Box 4337
Sydney, NSW
AUSTRALIA 2001

Phone 61 2 93827278
Mobile 0423 151614
FAX 61 2 93827318
On 4 Feb 2006, at 8:50 AM, dsoren-at-umich.edu wrote:

}
}
}
} -----------------------------------------------------------------------
} -----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} -----
}
} Dear listers,
}
} We have inherited lots of copper TEM grids that are decades old.
} Many of them have a dark patina on them, and, on those grids, we have
} been losing our sections during alcohol-based uranyl acetate
} staining. The sections remain when we use water-based uranyl
} acetate, however. We have tried sonicating the grids in ethanol,
} acetone, or chloroform, none of which has solved our problem.
}
} Does anyone have any suggestions for cleaning them so that we will
} not lose our sections during staining, or should we pitch them? We
} really do prefer to stain with alcohol- based uranyl acetate, since
} it provides a more intense staining.
}
} As always, thanks for any suggestions you might have.
}
} Dotty Sorenson
}
} Dorothy Sorenson
} Microscopy and Image-analysis Laboratory
} Department of Cell and Developmental Biology
} University Of Michigan Medical School
} 4643 Medical Science Building II
} 1301 Catherine
} Ann Arbor, MI 48109-0616
} (734)763-1170
} FAX (734)763-1166
}
}
} ==============================Original
} Headers==============================
} 7, 17 -- From dsoren-at-umich.edu Fri Feb 3 15:48:58 2006
} 7, 17 -- Received: from pushingtin.mr.itd.umich.edu
} (pushingtin.mr.itd.umich.edu [141.211.14.78])
} 7, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
} k13LmwV1020156
} 7, 17 -- for {microscopy-at-msa.microscopy.com} ; Fri, 3 Feb 2006
} 15:48:58 -0600
} 7, 17 -- Received: from [141.214.170.47]
} (host-47.subnet-170.med.umich.edu [141.214.170.47])
} 7, 17 -- by pushingtin.mr.itd.umich.edu (smtp) with ESMTP id
} k13LmvJZ004383;
} 7, 17 -- Fri, 3 Feb 2006 16:48:57 -0500
} 7, 17 -- Mime-Version: 1.0 (Apple Message framework v746.2)
} 7, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes;
} format=flowed
} 7, 17 -- Message-Id: {F847072A-3C48-4995-B594-5900360673CB-at-umich.edu}
} 7, 17 -- Cc: Sasha Meshinchi {meshin-at-umich.edu}
} 7, 17 -- Content-Transfer-Encoding: 7bit
} 7, 17 -- From: Dorothy Sorenson {dsoren-at-umich.edu}
} 7, 17 -- Subject: Cleaning TEM grids
} 7, 17 -- Date: Fri, 3 Feb 2006 16:47:15 -0500
} 7, 17 -- To: microscopy-at-msa.microscopy.com
} 7, 17 -- X-Mailer: Apple Mail (2.746.2)
} ==============================End of -
} Headers==============================
}


==============================Original Headers==============================
6, 20 -- From dianavd-at-eye.usyd.edu.au Sun Feb 5 22:06:54 2006
6, 20 -- Received: from galen.med.usyd.edu.au (machaon.med.usyd.edu.au [129.78.36.30])
6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1646r3u022150
6, 20 -- for {Microscopy-at-microscopy.com} ; Sun, 5 Feb 2006 22:06:53 -0600
6, 20 -- Received: from [129.78.203.144] (helo=[129.78.203.144])
6, 20 -- by galen.med.usyd.edu.au with esmtp (Exim 4.50)
6, 20 -- id 1F5xYY-0004NY-9a
6, 20 -- for Microscopy-at-microscopy.com; Mon, 06 Feb 2006 15:00:52 +1100
6, 20 -- Mime-Version: 1.0 (Apple Message framework v623)
6, 20 -- In-Reply-To: {200602032150.k13Loevb027732-at-ns.microscopy.com}
6, 20 -- References: {200602032150.k13Loevb027732-at-ns.microscopy.com}
6, 20 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
6, 20 -- Message-Id: {25a1f04e5a26d06c447521476709da97-at-eye.usyd.edu.au}
6, 20 -- Content-Transfer-Encoding: 7bit
6, 20 -- From: Diana van Driel {dianavd-at-eye.usyd.edu.au}
6, 20 -- Subject: Re: [Microscopy] Cleaning TEM grids
6, 20 -- Date: Mon, 6 Feb 2006 15:06:32 +1100
6, 20 -- To: Microscopy {Microscopy-at-microscopy.com}
6, 20 -- X-Mailer: Apple Mail (2.623)
6, 20 -- X-Spam-Score: -5.9 (-----)
==============================End of - Headers==============================




From: benada-at-biomed.cas.cz
Date: Mon, 6 Feb 2006 03:33:26 -0600
Subject: [Microscopy] Re: Cleaning TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dotty,
We were in the same situation some years ago. We cleaned the grids in the
following way:
- put the grids into small beaker (25ml) filled with 5% - 10% hydrochloric
acid
- let the grids to clean for some minutes, gently shake several times (at
the end of cleaning the grids should be shiny gold)
- remove the cleaning hydrochloric acid solution and wash the grids
several times with distilled water
- remove the distilled water as much as possible from the beaker and
replace it with acetone and let stand for some time
- pour the acetone with the grids into clean glass Petri dish filled with
filter paper
- remove the filter paper with the grids and put it into another glass
Petri dish and let dry out the rest of acetone
- that's all

The troubles with losing the sections during the staining could be
overcome by making the grids sticky:
- put 10 ml of chloroform into 25 ml glass beaker
- cut about 5 cm of 3M Magic Scotch tape and wash it in the chloroform
- remove the rest of the tape from the beaker
- put the grids onto filter paper and drop the "sticky solution" on them
using Pasteur pipette
- let the grids dry and use them for collecting the sections

Best regards from Prague
Oldrich
----------------------------------------
Oldrich Benada
Institute of Microbiology
Acad. Sci. CR
Videnska 1083
CZ - 142 20 Prague 4
Czech Republic
---------------------------------------




}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear listers,
}
} We have inherited lots of copper TEM grids that are decades old.
} Many of them have a dark patina on them, and, on those grids, we have
} been losing our sections during alcohol-based uranyl acetate
} staining. The sections remain when we use water-based uranyl
} acetate, however. We have tried sonicating the grids in ethanol,
} acetone, or chloroform, none of which has solved our problem.
}
} Does anyone have any suggestions for cleaning them so that we will
} not lose our sections during staining, or should we pitch them? We
} really do prefer to stain with alcohol- based uranyl acetate, since
} it provides a more intense staining.
}
} As always, thanks for any suggestions you might have.
}
} Dotty Sorenson
}
} Dorothy Sorenson
} Microscopy and Image-analysis Laboratory
} Department of Cell and Developmental Biology
} University Of Michigan Medical School
} 4643 Medical Science Building II
} 1301 Catherine
} Ann Arbor, MI 48109-0616
} (734)763-1170
} FAX (734)763-1166
}
}
} ==============================Original
} Headers==============================
} 7, 17 -- From dsoren-at-umich.edu Fri Feb 3 15:48:58 2006
} 7, 17 -- Received: from pushingtin.mr.itd.umich.edu
} (pushingtin.mr.itd.umich.edu [141.211.14.78])
} 7, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
} k13LmwV1020156
} 7, 17 -- for {microscopy-at-msa.microscopy.com} ; Fri, 3 Feb 2006 15:48:58
} -0600
} 7, 17 -- Received: from [141.214.170.47] (host-47.subnet-170.med.umich.edu
} [141.214.170.47])
} 7, 17 -- by pushingtin.mr.itd.umich.edu (smtp) with ESMTP id
} k13LmvJZ004383;
} 7, 17 -- Fri, 3 Feb 2006 16:48:57 -0500
} 7, 17 -- Mime-Version: 1.0 (Apple Message framework v746.2)
} 7, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes;
} format=flowed
} 7, 17 -- Message-Id: {F847072A-3C48-4995-B594-5900360673CB-at-umich.edu}
} 7, 17 -- Cc: Sasha Meshinchi {meshin-at-umich.edu}
} 7, 17 -- Content-Transfer-Encoding: 7bit
} 7, 17 -- From: Dorothy Sorenson {dsoren-at-umich.edu}
} 7, 17 -- Subject: Cleaning TEM grids
} 7, 17 -- Date: Fri, 3 Feb 2006 16:47:15 -0500
} 7, 17 -- To: microscopy-at-msa.microscopy.com
} 7, 17 -- X-Mailer: Apple Mail (2.746.2)
} ==============================End of -
} Headers==============================
}



==============================Original Headers==============================
9, 25 -- From benada-at-biomed.cas.cz Mon Feb 6 03:33:26 2006
9, 25 -- Received: from biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32])
9, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k169XPV1003261
9, 25 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2006 03:33:26 -0600
9, 25 -- Received: from mail2.biomed.cas.cz (localhost [127.0.0.1])
9, 25 -- by biomed.cas.cz (8.13.3+Sun/8.13.3) with ESMTP id k169V0cA006059;
9, 25 -- Mon, 6 Feb 2006 10:31:00 +0100 (CET)
9, 25 -- Received: from 147.231.44.104
9, 25 -- (SquirrelMail authenticated user benada)
9, 25 -- by mail2.biomed.cas.cz with HTTP;
9, 25 -- Mon, 6 Feb 2006 10:31:01 +0100 (CET)
9, 25 -- Message-ID: {1280.147.231.44.104.1139218261.squirrel-at-mail2.biomed.cas.cz}
9, 25 -- In-Reply-To: {200602032152.k13LqKBS002784-at-ns.microscopy.com}
9, 25 -- References: {200602032152.k13LqKBS002784-at-ns.microscopy.com}
9, 25 -- Date: Mon, 6 Feb 2006 10:31:01 +0100 (CET)
9, 25 -- Subject: Re: [Microscopy] Cleaning TEM grids
9, 25 -- From: =?iso-8859-2?Q?Old=F8ich_Benada?= {benada-at-biomed.cas.cz}
9, 25 -- To: dsoren-at-umich.edu
9, 25 -- Cc: microscopy-at-microscopy.com
9, 25 -- User-Agent: SquirrelMail/1.4.4
9, 25 -- MIME-Version: 1.0
9, 25 -- Content-Type: text/plain;charset=iso-8859-2
9, 25 -- Content-Transfer-Encoding: 8bit
9, 25 -- X-Priority: 3 (Normal)
9, 25 -- Importance: Normal
==============================End of - Headers==============================




From: dsams-at-schaferlabs.com
Date: Mon, 6 Feb 2006 19:16:53 -0600
Subject: [Microscopy] Mass Spectrometry Scientist / Senior Engineer at Schafer Corporation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Overview: Schafer Corporation, Sunol, California, is seeking a skilled and
innovative individual to add to our mass spectrometry group. SVL performs
materials characterization and related analytical services on commercial and
government contracts. The activities of the group include chemical and
elemental analysis of materials on a production basis, maintenance of
several mass spectrometers and ancillary equipment, development of new or
improved MS analysis techniques, and quality assurance of analytical data.

Schafer is a technically strong firm with a reputation for quality and
integrity. Schafer's reputation is a direct result of our dedicated,
motivated, talented, and creative staff that is responsible for developing
our outstanding business relationships with our customers. Our technical
capabilities are vast and growing to provide innovations for the future.

The successful candidate will work as part of a team responsible for
processing and analyzing small samples using laboratory instrumentation,
primarily Thermal Ionization and/or Secondary Ion Mass Spectrometers.

Responsibilities: Duties include instrument operation and data
interpretation; filament construction and preparation; standards loading; as
well as high vacuum, high voltage, and cryogenic system maintenance. The
successful candidate should have or be able to develop skills to process
samples, maintain equipment, and assist in developing and optimizing
analytical techniques.

Qualifications: The ideal candidate will have technical training in an
appropriate physical sciences field and related experience in a scientific
laboratory, preferably operating mass spectrometers or other analytical
equipment. Ability to work independently as well as part of a team is
required. Good customer service focus and commitment to quality are
required. Experience in the use of a light microscope, and sufficient
physical dexterity to perform micromanipulation tasks is highly desired, as
is knowledge of high vacuum and cryogenic principles.
Schafer is looking for people that have established safe laboratory skills
and exceptional aptitude for details including accurate record keeping.
Experience in laboratory data management (word processing, spread sheets and
databases) is also desired. Experience with mechanical systems or machining
skills would be helpful.
Other qualifications include:
. AA degree plus 10 years experience or Bachelor's degree in physical
science or engineering plus two years of technical experience.
. Demonstrated ability to solve technical problems.
. Experience in data evaluation and quality control.
. Must be a US citizen with the ability to obtain government security
clearance.
Apply at:
http://www.schafercorp.com/Careers/open.htm
http://jobs-schafer.icims.com/schafer_jobs/jobs/candidate/job.jsp?jobid=1106
&mode=view


==============================Original Headers==============================
6, 20 -- From dsams-at-schaferlabs.com Mon Feb 6 19:16:53 2006
6, 20 -- Received: from mail.schaferlabs.com (mail.schaferlabs.com [64.168.91.154])
6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k171GkxV006745
6, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Feb 2006 19:16:52 -0600
6, 20 -- Received: from dsamsws ([10.10.10.1])
6, 20 -- by mail.schaferlabs.com (8.12.11/8.12.11) with ESMTP id k171ANrk020002
6, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Feb 2006 17:10:34 -0800
6, 20 -- Message-Id: {200602070110.k171ANrk020002-at-mail.schaferlabs.com}
6, 20 -- From: "David Sams" {dsams-at-schaferlabs.com}
6, 20 -- To: {Microscopy-at-microscopy.com}
6, 20 -- Subject: Mass Spectrometry Scientist / Senior Engineer at Schafer Corporation
6, 20 -- Date: Mon, 6 Feb 2006 17:16:18 -0800
6, 20 -- MIME-Version: 1.0
6, 20 -- Content-Type: text/plain;
6, 20 -- charset="US-ASCII"
6, 20 -- Content-Transfer-Encoding: 7bit
6, 20 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353
6, 20 -- In-reply-to:
6, 20 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2670
6, 20 -- Thread-Index: AcVrdaWs9qR/q27tSgm/q8Axr3R/miwGADdQAAH4L3AAM6jdwAPH8fGQ
==============================End of - Headers==============================




From: RANGETS-at-AOL.COM
Date: Mon, 6 Feb 2006 22:27:40 -0600
Subject: [Microscopy] viaWWW: NEED SCHEMATICS LEO 440

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both RANGETS-at-AOL.COM as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: RANGETS-at-AOL.COM
Name: BEN GHAFFAARI

Organization: RANGE TECHNICAL

Title-Subject: [Filtered] NEED SCHEMATICS

Question: DOES ANYONE HAVE AN EXTRA SET OF "LEO" 440
SCHEMATICS, WE CAN BUY OR PURCHASE, WHEN WE TRY
TO CONTACT THE MFR, WE DO NOT GET A RESPONSE, SO
MAYBE WE ARE USING AN INCORRECT CONTACT. IF
SOMEONE KNOWS WHO WE CONTACT FOR "LEO" PARTS
ANY SUGGESTIONS WILL BE APPRECIATED

BEN

---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Mon Feb 6 22:27:40 2006
7, 12 -- Received: from [192.168.0.29] (msdvpn27.msd.anl.gov [130.202.238.91])
7, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k174RbvG018394
7, 12 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2006 22:27:39 -0600
7, 12 -- Mime-Version: 1.0
7, 12 -- X-Sender: (Unverified)
7, 12 -- Message-Id: {p06020401c00dd2278bca-at-[203.33.121.121]}
7, 12 -- Date: Tue, 7 Feb 2006 15:27:39 +1100
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: RANGETS-at-AOL.COM (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: NEED SCHEMATICS LEO 440
7, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: mcmahojt-at-ccf.org
Date: Mon, 6 Feb 2006 22:31:10 -0600
Subject: [Microscopy] viaWWW: Spurr Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mcmahojt-at-ccf.org as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mcmahojt-at-ccf.org
Name: Jim McMahon

Organization: Cleveland Clinic Foundation

Title-Subject: [Filtered] Spurr Resin

Question: We have been having great difficulty with the newly
formulated Spurr Resin and in particular the ERL component. Not only
is Spurr no longer low viscosity but we find that its penetration and
polymerization to be far inferior to the original. We are prepared
to abandon the resin in favor of another such as PolyBed or Araldite.
But first I would like to know if anyone else has had similar
problems and was able to solve them.

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Mon Feb 6 22:31:10 2006
6, 12 -- Received: from [192.168.0.29] (msdvpn27.msd.anl.gov [130.202.238.91])
6, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k174V8OU022423
6, 12 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2006 22:31:09 -0600
6, 12 -- Mime-Version: 1.0
6, 12 -- X-Sender: (Unverified)
6, 12 -- Message-Id: {p06020403c00dd302bf15-at-[192.168.0.29]}
6, 12 -- Date: Tue, 7 Feb 2006 15:31:10 +1100
6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: mcmahojt-at-ccf.org (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Spurr Resin
6, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Mon, 6 Feb 2006 23:49:50 -0600
Subject: [Microscopy] Re: viaWWW: Spurr Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

jim

for what it is worth, i've recently re-tried a commercial version of
Epon 812 on a cell suspension. i found the cells would not settle
through the resin mix, and could not be pelleted. a parallel embedment
in the new Spurr with ERL behaved very well. in short, whatever is said
about low viscosity editions of Epon, neither they, nor the original
were ever thin enough to allow embedments of free cells and i personally
found them more than a little difficult to cut. but then i've always
found Epon, in whatever formulation, prone to static and difficult to
section.

on the other hand, the problem may really be an issue of density of the
medium, causing the the cells to be bouyant in the medium. does anyone
want to contribute knowledge on that?

having said that, i do find the new ERL component to be a little
brittle, but very sectionable.

paul



==============================Original Headers==============================
7, 21 -- From paul_hazelton-at-umanitoba.ca Mon Feb 6 23:49:49 2006
7, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23])
7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k175niDC005523
7, 21 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2006 23:49:48 -0600
7, 21 -- Received: from [130.179.152.145] (cvx-082.cc.umanitoba.ca [130.179.152.145])
7, 21 -- (authenticated bits=0)
7, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id k175ndFk026699;
7, 21 -- Mon, 6 Feb 2006 23:49:40 -0600 (CST)
7, 21 -- Message-ID: {43E9865F.3010305-at-umanitoba.ca}
7, 21 -- Date: Tue, 07 Feb 2006 23:49:19 -0600
7, 21 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca}
7, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax)
7, 21 -- X-Accept-Language: en-us, en
7, 21 -- MIME-Version: 1.0
7, 21 -- To: mcmahojt-at-ccf.org
7, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com}
7, 21 -- Subject: Re: [Microscopy] viaWWW: Spurr Resin
7, 21 -- References: {200602070432.k174Wx2a027920-at-ns.microscopy.com}
7, 21 -- In-Reply-To: {200602070432.k174Wx2a027920-at-ns.microscopy.com}
7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 21 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: M_Jarnik-at-fccc.edu
Date: Tue, 7 Feb 2006 08:35:31 -0600
Subject: [Microscopy] Poly-L-lysine coated coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We would need some to improve cell attachment for a time course
experiment. Surprisingly, we were not able to locate any coverslips
(unlike slides). Does anybody have recommendations? (Of course, we can
always make our own.)

Thanks,

Michael




==============================Original Headers==============================
6, 19 -- From M_Jarnik-at-fccc.edu Tue Feb 7 08:35:31 2006
6, 19 -- Received: from azah.fccc.edu (azah.fccc.edu [131.249.4.237])
6, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k17EZVJJ022275
6, 19 -- for {microscopy-at-msa.microscopy.com} ; Tue, 7 Feb 2006 08:35:31 -0600
6, 19 -- Received: from fccc.edu (emf4.fccc.edu [131.249.9.170])
6, 19 -- by azah.fccc.edu (8.12.11/8.12.11) with ESMTP id k17EZU5d016976
6, 19 -- for {microscopy-at-msa.microscopy.com} ; Tue, 7 Feb 2006 09:35:31 -0500 (EST)
6, 19 -- Message-ID: {43E8B032.7060002-at-fccc.edu}
6, 19 -- Date: Tue, 07 Feb 2006 09:35:30 -0500
6, 19 -- From: Michal Jarnik {M_Jarnik-at-fccc.edu}
6, 19 -- Reply-To: M_Jarnik-at-fccc.edu
6, 19 -- Organization: Fox Chase Cancer Center
6, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.4) Gecko/20030624 Netscape/7.1
6, 19 -- X-Accept-Language: en,cs
6, 19 -- MIME-Version: 1.0
6, 19 -- To: microscopy-at-msa.microscopy.com
6, 19 -- Subject: Poly-L-lysine coated coverslips
6, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed
6, 19 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: mcauliff-at-umdnj.edu
Date: Tue, 7 Feb 2006 08:43:50 -0600
Subject: [Microscopy] Re: Spurr Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I have recently encountered problems with Spurr as well. I embed 100
micron thick Vibratome sections of brain so penetration should not be a
problem. I am using the same processing proceedure I have used for many,
many years on much larger tissue blocks. The center of the sections
seems to be poorly infiltrated and the block is very brittle. Cutting
intact 1 micron sections is nearly impossible. The viscosity seems
'normal', in other words, like I am used to with Spurr and I am not
familiar with any 'new' or 'improved' version of the mix or its
components. I will cut some more blocks and talk to my supplier (a very
reputable EM supplier I have used for 30 years) to see if I can shed
some light on this problem.

Geoff

mcmahojt-at-ccf.org wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



==============================Original Headers==============================
8, 32 -- From mcauliff-at-umdnj.edu Tue Feb 7 08:43:50 2006
8, 32 -- Received: from zix02.umdnj.edu (zix02.UMDNJ.EDU [130.219.34.125])
8, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k17Ehnhp031730
8, 32 -- for {microscopy-at-msa.microscopy.com} ; Tue, 7 Feb 2006 08:43:49 -0600
8, 32 -- Received: from zix02.umdnj.edu (ZixVPM [127.0.0.1])
8, 32 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 9D1FD4BE3B
8, 32 -- for {microscopy-at-msa.microscopy.com} ; Tue, 7 Feb 2006 08:43:49 -0600 (CST)
8, 32 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131])
8, 32 -- by zix02.umdnj.edu (Proprietary) with ESMTP id 59FA44BE34
8, 32 -- for {microscopy-at-msa.microscopy.com} ; Tue, 7 Feb 2006 08:43:48 -0600 (CST)
8, 32 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu
8, 32 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004))
8, 32 -- id {0IUB00D01MP310-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu)
8, 32 -- for microscopy-at-msa.microscopy.com; Tue, 07 Feb 2006 09:43:44 -0500 (EST)
8, 32 -- Received: from [127.0.0.1] ([10.138.2.240])
8, 32 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21
8, 32 -- 2004)) with ESMTP id {0IUB00BK4NAVAK-at-Polaris.umdnj.edu} ; Tue,
8, 32 -- 07 Feb 2006 09:37:43 -0500 (EST)
8, 32 -- Date: Tue, 07 Feb 2006 09:37:06 -0500
8, 32 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
8, 32 -- Subject: Re: [Microscopy] Spurr Resin
8, 32 -- In-reply-to: {200602070432.k174W3M3025365-at-ns.microscopy.com}
8, 32 -- To: mcmahojt-at-ccf.org, MicroscopyListserver {microscopy-at-msa.microscopy.com} ,
8, 32 -- paul_hazelton-at-umanitoba.ca
8, 32 -- Message-id: {43E8B092.8030903-at-umdnj.edu}
8, 32 -- MIME-version: 1.0
8, 32 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1
8, 32 -- Content-transfer-encoding: 7BIT
8, 32 -- X-Accept-Language: en-us, en
8, 32 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2)
8, 32 -- Gecko/20040804 Netscape/7.2 (ax)
8, 32 -- References: {200602070432.k174W3M3025365-at-ns.microscopy.com}
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Tue, 7 Feb 2006 08:53:50 -0600
Subject: [Microscopy] Re: Poly-L-lysine coated coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Michael

Depends on what you want to do. If it is for EM you can use the
Thermonox coverslips. I think all EM suppliers sell them, along with
the general suppliers.

However, if you want to do IF or confocal work the thermonox will quench
the reactions somewhat so you will have to do glass. I've used straight
glass, but many cell lines will not stick well to glass. Undoubtably
someone will provide the name of a supplier of pre-treated slips if they
are out there somewhere.

paul


Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926




==============================Original Headers==============================
9, 21 -- From paul_hazelton-at-umanitoba.ca Tue Feb 7 08:53:50 2006
9, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23])
9, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k17ErnB5008760
9, 21 -- for {microscopy-at-microscopy.com} ; Tue, 7 Feb 2006 08:53:49 -0600
9, 21 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160])
9, 21 -- (authenticated bits=0)
9, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id k17ErhaH011897;
9, 21 -- Tue, 7 Feb 2006 08:53:44 -0600 (CST)
9, 21 -- Message-ID: {43E8B474.8040005-at-umanitoba.ca}
9, 21 -- Date: Tue, 07 Feb 2006 08:53:40 -0600
9, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
9, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax)
9, 21 -- X-Accept-Language: en-us, en
9, 21 -- MIME-Version: 1.0
9, 21 -- To: M_Jarnik-at-fccc.edu
9, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com}
9, 21 -- Subject: Re: [Microscopy] Poly-L-lysine coated coverslips
9, 21 -- References: {200602071438.k17EcWLj026119-at-ns.microscopy.com}
9, 21 -- In-Reply-To: {200602071438.k17EcWLj026119-at-ns.microscopy.com}
9, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
9, 21 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: DRK-at-SHCC.org
Date: Tue, 7 Feb 2006 09:55:36 -0600
Subject: [Microscopy] viaWWW: Spurr Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Regarding the brittle nature of Spurrs resin due to the new ERL component,
we have solved our problems by combining the components according to the
"soft" recipe:

ERL 10g
DER 7g
NSA 26g
DMAE 0.4ml

This mixture results in blocks about as hard as the old "hard" formulation
using VCD. We have not noticed a lack of tissue penetration during
infiltration, but we may be less sensitive to this issue. Polymerization is
still at 70 deg C for 17 hours.

Best wishes,

Doug


Douglas R. Keene
Assistant Investigator
Micro-Imaging Center
Shriners Hospital for Children
3102 S.W. Sam Jackson Park Road
Portland, Oregon 97239
503-221-3434
drk-at-shcc.org

-----Original Message-----
X-from: mcmahojt-at-ccf.org [mailto:mcmahojt-at-ccf.org]
Sent: Monday, February 06, 2006 8:38 PM
To: drk-at-SHCC.org

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mcmahojt-at-ccf.org as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mcmahojt-at-ccf.org
Name: Jim McMahon

Organization: Cleveland Clinic Foundation

Title-Subject: [Filtered] Spurr Resin

Question: We have been having great difficulty with the newly
formulated Spurr Resin and in particular the ERL component. Not only
is Spurr no longer low viscosity but we find that its penetration and
polymerization to be far inferior to the original. We are prepared
to abandon the resin in favor of another such as PolyBed or Araldite.
But first I would like to know if anyone else has had similar
problems and was able to solve them.

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Mon Feb 6 22:31:10 2006
6, 12 -- Received: from [192.168.0.29] (msdvpn27.msd.anl.gov
[130.202.238.91])
6, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
k174V8OU022423
6, 12 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2006 22:31:09
-0600
6, 12 -- Mime-Version: 1.0
6, 12 -- X-Sender: (Unverified)
6, 12 -- Message-Id: {p06020403c00dd302bf15-at-[192.168.0.29]}
6, 12 -- Date: Tue, 7 Feb 2006 15:31:10 +1100
6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: mcmahojt-at-ccf.org (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Spurr Resin
6, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================


==============================Original Headers==============================
19, 21 -- From DRK-at-SHCC.org Tue Feb 7 09:55:31 2006
19, 21 -- Received: from shcc.org (mail.shcc.org [64.213.211.200])
19, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k17FtUia019073
19, 21 -- for {microscopy-at-microscopy.com} ; Tue, 7 Feb 2006 09:55:31 -0600
19, 21 -- Received: from DRK2 ("port 1330"-at-[64.213.211.3]) by SHCC.org (PMDF V6.1 #37411)
19, 21 -- with ESMTPA id {01LYOJA4JBAK001VP7-at-SHCC.org} for microscopy-at-microscopy.com;
19, 21 -- Tue, 07 Feb 2006 08:59:06 -0800 (PST)
19, 21 -- Date: Tue, 07 Feb 2006 07:52:26 -0800
19, 21 -- From: Doug Keene {DRK-at-SHCC.org}
19, 21 -- Subject: RE: [Microscopy] viaWWW: Spurr Resin
19, 21 -- In-reply-to: {200602070437.k174bXKq003143-at-ns.microscopy.com}
19, 21 -- To: mcmahojt-at-ccf.org
19, 21 -- Cc: microscopy-at-microscopy.com
19, 21 -- Reply-to: DRK-at-SHCC.org
19, 21 -- Message-id: {01LYOJA4NREM001VP7-at-SHCC.org}
19, 21 -- MIME-version: 1.0
19, 21 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
19, 21 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
19, 21 -- Content-type: text/plain; charset=us-ascii
19, 21 -- Content-transfer-encoding: 7BIT
19, 21 -- thread-index: AcYrqTjbOOT1TTgQRKCa9lWcvhqTMQAUyp7Q
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 7 Feb 2006 10:06:45 -0600
Subject: [Microscopy] Re: viaWWW: NEED SCHEMATICS LEO 440

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I think that they are responding in their own way.
Their answer is "No."

gary g.


At 08:30 PM 2/6/2006, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 20 -- From gary-at-gaugler.com Tue Feb 7 10:06:45 2006
9, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
9, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k17G6jtp028532
9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 7 Feb 2006 10:06:45 -0600
9, 20 -- Received: (qmail 12166 invoked from network); 7 Feb 2006 08:06:43 -0800
9, 20 -- Received: by simscan 1.1.0 ppid: 12163, pid: 12164, t: 0.1441s
9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211)
9, 20 -- by qsmtp1 with SMTP; 7 Feb 2006 08:06:43 -0800
9, 20 -- Message-Id: {6.2.3.4.2.20060207080513.02057d00-at-mail.calweb.com}
9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4
9, 20 -- Date: Tue, 07 Feb 2006 08:06:43 -0800
9, 20 -- To: RANGETS-at-AOL.COM
9, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 20 -- Subject: Re: [Microscopy] viaWWW: NEED SCHEMATICS LEO 440
9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 20 -- In-Reply-To: {200602070430.k174UXNn021696-at-ns.microscopy.com}
9, 20 -- References: {200602070430.k174UXNn021696-at-ns.microscopy.com}
9, 20 -- Mime-Version: 1.0
9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: scanning-at-fams.org
Date: Tue, 7 Feb 2006 12:54:34 -0600
Subject: [Microscopy] Scanning 2006 Abstract Deadline is February 18

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Group:

Kindly note the abstract submission deadline for SCANNING 2006 is
February 18. We hope to see you April 25-27 in D.C.!

Best regards,


Phaedra McGuinness
Managing Editor
SCANNING, The Journal of Scanning Microscopies
P.O. Box 485
Mahwah, NJ 07430
Tel: (201) 818-1010 * Fax: (201) 818-0086 *email: scanning-at-fams.org *
Web: www.scanning.org


==============================Original Headers==============================
6, 19 -- From scanning-at-fams.org Tue Feb 7 12:54:34 2006
6, 19 -- Received: from smtp-out2.oct.nac.net (smtp-out2.oct.nac.net [209.123.233.212])
6, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k17IsYVs008135
6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 7 Feb 2006 12:54:34 -0600
6, 19 -- Received: (qmail 72022 invoked from network); 7 Feb 2006 13:54:33 -0500
6, 19 -- Received: from unknown (HELO mail1.oct.nac.net) (209.123.233.241)
6, 19 -- by smtp-out2.oct.nac.net with SMTP; 7 Feb 2006 13:54:33 -0500
6, 19 -- Received: (qmail 80056 invoked from network); 7 Feb 2006 13:54:33 -0500
6, 19 -- Received: from unknown (HELO ?192.168.2.80?) (64.21.7.106)
6, 19 -- by mail1.oct.nac.net with SMTP; 7 Feb 2006 13:54:33 -0500
6, 19 -- Mime-Version: 1.0 (Apple Message framework v622)
6, 19 -- Content-Transfer-Encoding: 7bit
6, 19 -- Message-Id: {271a9b00f9889c59604a343d2719e43b-at-fams.org}
6, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
6, 19 -- To: Microscopy List Server Server {Microscopy-at-MSA.Microscopy.Com}
6, 19 -- From: Phaedra McGuinness {scanning-at-fams.org}
6, 19 -- Subject: Scanning 2006 Abstract Deadline is February 18
6, 19 -- Date: Tue, 7 Feb 2006 13:54:33 -0500
6, 19 -- X-Mailer: Apple Mail (2.622)
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Tue, 7 Feb 2006 15:19:26 -0600
Subject: [Microscopy] viaWWW: Spurr Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

doug

what you recommend in your note is the logical solution. actually, i
had meant to try the same change for my last embedment of monolayers to
correct for the hardness/brittleness. unfortunately, i got distracted
and forgot. i suspect that to get the medium hardness you may need 8-9
ml of the ERL component.

also, i used to use 0.4gm DMAE but changed that to .3gm to give a longer
pot life. also, higher catalyst concentration could have an effect on
viscosity and penetration.

paul



Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926




==============================Original Headers==============================
10, 21 -- From paul_hazelton-at-umanitoba.ca Tue Feb 7 15:19:25 2006
10, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23])
10, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k17LJPwp019538
10, 21 -- for {microscopy-at-microscopy.com} ; Tue, 7 Feb 2006 15:19:25 -0600
10, 21 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160])
10, 21 -- (authenticated bits=0)
10, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id k17LJFkD028260;
10, 21 -- Tue, 7 Feb 2006 15:19:24 -0600 (CST)
10, 21 -- Message-ID: {43E90ED0.7030901-at-umanitoba.ca}
10, 21 -- Date: Tue, 07 Feb 2006 15:19:12 -0600
10, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
10, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax)
10, 21 -- X-Accept-Language: en-us, en
10, 21 -- MIME-Version: 1.0
10, 21 -- To: DRK-at-SHCC.org
10, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com}
10, 21 -- Subject: Re: [Microscopy] RE: viaWWW: Spurr Resin
10, 21 -- References: {200602071558.k17Fw6YT022905-at-ns.microscopy.com}
10, 21 -- In-Reply-To: {200602071558.k17Fw6YT022905-at-ns.microscopy.com}
10, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
10, 21 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: WAHeeschen-at-dow.com
Date: Tue, 7 Feb 2006 16:05:53 -0600
Subject: [Microscopy] Job Posting from The Dow Chemical Company

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Job Posting from The Dow Chemical Company

An immediate opening exists for a skilled microscopist in Dow's Global
Analytical Sciences Laboratory part of Dow's Corporate R&D function. We are
seeking an individual with a passion for characterization science and a
desire to both apply this science to complex industrial problems and to
advance the technology. A Ph.D. in science is preferred, but an individual
with a Masters degree and relevant work experience will also be considered.
Working experience in electron microscopy is essential with skills in
scanning and transmission electron microscopy, focused ion beam methods,
energy dispersive x-ray analysis, electron energy loss spectroscopy,
electron diffraction and microtomy preferred. A background in catalysis is
a plus. Excellent written and oral communication skills (English) are
essential with an ability to work in a globally diverse team environment.
The position supports new product development activities working from a
laboratory with a FEG TEM-STEM, 2 TEMs, a FIBSEM, EPMA, FEGSEM with
cryotransfer system, a full complement of scanned probe and light
microscopes, SAXS, XPS and TOF-SIMS. The position is located in Midland,
Michigan. Applicants must have the ability to work in the USA.

Dow is a leader in science and technology, providing innovative chemical,
plastic and agricultural products and services to many essential consumer
markets. With annual sales of $40 billion, Dow serves customers in 175
countries and a wide range of markets that are vital to human progress:
food, transportation, health and medicine, personal and home care, and
building and construction, among others. Committed to the principles of
sustainable development, Dow and its 43,000 employees seek to balance
economic, environmental and social responsibilities. References to "Dow" or
the "Company" mean The Dow Chemical Company and its consolidated
subsidiaries unless otherwise expressly noted.

Please submit curriculum vitae and a list of references to Dr. John
Blackson, Building 1897, The Dow Chemical Company, Midland, MI 48667.

Best Regards,
Bill
William A. Heeschen, Ph.D.
Microscopy, Digital Imaging
The Dow Chemical Company
Midland, MI 48674
waheeschen-at-dow.com



==============================Original Headers==============================
7, 23 -- From WAHeeschen-at-dow.com Tue Feb 7 16:05:53 2006
7, 23 -- Received: from mail86.messagelabs.com (mail86.messagelabs.com [216.82.244.115])
7, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k17M5q3V029468
7, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Feb 2006 16:05:52 -0600
7, 23 -- X-VirusChecked: Checked
7, 23 -- X-Env-Sender: WAHeeschen-at-dow.com
7, 23 -- X-Msg-Ref: server-4.tower-86.messagelabs.com!1139349950!29545829!1
7, 23 -- X-StarScan-Version: 5.5.9.1; banners=-,-,-
7, 23 -- X-Originating-IP: [204.136.184.23]
7, 23 -- Received: (qmail 26574 invoked from network); 7 Feb 2006 22:05:51 -0000
7, 23 -- Received: from mail6.dow.com (HELO txnte41.nam.dow.com) (204.136.184.23)
7, 23 -- by server-4.tower-86.messagelabs.com with SMTP; 7 Feb 2006 22:05:51 -0000
7, 23 -- Received: by TXNTE41.nam.dow.com with Internet Mail Service (5.5.2658.3)
7, 23 -- id {1A02KCZD} ; Tue, 7 Feb 2006 16:05:50 -0600
7, 23 -- Message-ID: {CC55EF96AD3142438EF779F71571702084A20F-at-USMDLMDOWX004.dow.com}
7, 23 -- From: "Heeschen, Bill (WA)" {WAHeeschen-at-dow.com}
7, 23 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
7, 23 -- Subject: Job Posting from The Dow Chemical Company
7, 23 -- Date: Tue, 7 Feb 2006 16:05:31 -0600
7, 23 -- Expiry-Date: Tue, 6 Feb 2007 23:00:00 -0600
7, 23 -- MIME-Version: 1.0
7, 23 -- X-Mailer: Internet Mail Service (5.5.2658.3)
7, 23 -- Content-Type: text/plain
==============================End of - Headers==============================




From: scanning-at-fams.org
Date: Wed, 8 Feb 2006 08:04:08 -0600
Subject: [Microscopy] Abstract deadline for Scanning 2006

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Group:

Kindly note the abstract submission deadline for SCANNING 2006 is
February 18. We hope to see you April 25-27 in D.C.!

Best regards,


Phaedra McGuinness
Managing Editor
SCANNING, The Journal of Scanning Microscopies
P.O. Box 485
Mahwah, NJ 07430
Tel: (201) 818-1010 * Fax: (201) 818-0086 *email: scanning-at-fams.org *
Web: www.scanning.org


==============================Original Headers==============================
6, 19 -- From scanning-at-fams.org Wed Feb 8 08:04:08 2006
6, 19 -- Received: from smtp-out2.oct.nac.net (smtp-out2.oct.nac.net [209.123.233.212])
6, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k18E442Y016353
6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 8 Feb 2006 08:04:07 -0600
6, 19 -- Received: (qmail 75351 invoked from network); 8 Feb 2006 09:04:03 -0500
6, 19 -- Received: from unknown (HELO mail1.oct.nac.net) (209.123.233.241)
6, 19 -- by smtp-out2.oct.nac.net with SMTP; 8 Feb 2006 09:04:03 -0500
6, 19 -- Received: (qmail 57187 invoked from network); 8 Feb 2006 09:04:03 -0500
6, 19 -- Received: from unknown (HELO ?192.168.2.80?) (64.21.7.106)
6, 19 -- by mail1.oct.nac.net with SMTP; 8 Feb 2006 09:04:03 -0500
6, 19 -- Mime-Version: 1.0 (Apple Message framework v622)
6, 19 -- Content-Transfer-Encoding: 7bit
6, 19 -- Message-Id: {6ce4cc26ff1a65ca7cb029c9867f9bab-at-fams.org}
6, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
6, 19 -- To: Microscopy List Server Server {Microscopy-at-MSA.Microscopy.Com}
6, 19 -- From: Phaedra McGuinness {scanning-at-fams.org}
6, 19 -- Subject: Abstract deadline for Scanning 2006
6, 19 -- Date: Wed, 8 Feb 2006 08:58:37 -0500
6, 19 -- X-Mailer: Apple Mail (2.622)
==============================End of - Headers==============================




From: mcauliff-at-umdnj.edu
Date: Wed, 8 Feb 2006 15:25:23 -0600
Subject: [Microscopy] Spurr's resin update

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings all:

I just got off the phone with Stacy (the owner) at Electron
Microscopy Sciences. I discussed my recent problems with Spurr's resin
with her. She told me that one component, ERL 4206, is no longer
maunfactured due to its high toxicity. It has been replaced with ERL
4221. She thinks that this is the cause of recent problems with Spurr
embedments. She has discussed this with others (I did not ask who) and
the consensus recommendations are to prolong dehydration in graded
ethanols to 20 minutes each step, make the 1:1 prop. oxide:Spurr step 4
hours and make the first change of pure resin 6 hours or longer. Since
longer times in solvents are known to extract cytoplasmic components
(and I cannot imaging that a 100 micron Vibratome section of CNS needs
20 min. per change of graded ethanol) I am going back to Epon
substitutes. Also, my tissues are in a second change of fresh resin on a
rotator overnight and then spend at least 1 hour under vacuum, I can't
see how infiltration could be a problem. I have used both the soft and
firm mixtures with different tissues and had inconsistant block textures
and infiltration problems with both. I don't have these problems with an
Araldite kit I bought at the same time. I can't risk knock-out mice in
long-term treatment/recovery experiments to reagents I cannot be sure of.

Geoff

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



==============================Original Headers==============================
6, 29 -- From mcauliff-at-umdnj.edu Wed Feb 8 15:25:23 2006
6, 29 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126])
6, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k18LPNGG000454
6, 29 -- for {microscopy-at-msa.microscopy.com} ; Wed, 8 Feb 2006 15:25:23 -0600
6, 29 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1])
6, 29 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id BC9CB4BE54
6, 29 -- for {microscopy-at-msa.microscopy.com} ; Wed, 8 Feb 2006 16:25:22 -0500 (EST)
6, 29 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131])
6, 29 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 063404BE5C
6, 29 -- for {microscopy-at-msa.microscopy.com} ; Wed, 8 Feb 2006 16:25:22 -0500 (EST)
6, 29 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu
6, 29 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004))
6, 29 -- id {0IUD00E01ZO0X6-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu)
6, 29 -- for microscopy-at-msa.microscopy.com; Wed, 08 Feb 2006 16:25:21 -0500 (EST)
6, 29 -- Received: from [127.0.0.1] ([10.138.2.240])
6, 29 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21
6, 29 -- 2004)) with ESMTP id {0IUD00DAGZSNRH-at-Polaris.umdnj.edu} for
6, 29 -- microscopy-at-msa.microscopy.com; Wed, 08 Feb 2006 16:02:47 -0500 (EST)
6, 29 -- Date: Wed, 08 Feb 2006 16:02:08 -0500
6, 29 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
6, 29 -- Subject: Spurr's resin update
6, 29 -- To: MicroscopyListserver {microscopy-at-msa.microscopy.com}
6, 29 -- Message-id: {43EA5C50.6070607-at-umdnj.edu}
6, 29 -- MIME-version: 1.0
6, 29 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1
6, 29 -- Content-transfer-encoding: 7BIT
6, 29 -- X-Accept-Language: en-us, en
6, 29 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2)
6, 29 -- Gecko/20040804 Netscape/7.2 (ax)
==============================End of - Headers==============================




From: Daniel.Salamon-at-nrc-cnrc.gc.ca
Date: Wed, 8 Feb 2006 15:33:09 -0600
Subject: [Microscopy] S4800 STEM scintillator size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,

I will be experimenting with alternative scintillators for our Hitachi
S4800 STEM unit. As I was advised to no touch the scintillator in the
microscope, I hope someone will be able to offer measurements for the
original S4800 scintillator.

Thanks,
Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC
W6-017A ECERF Bldg, 9107-116 Street
Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632



==============================Original Headers==============================
5, 23 -- From Daniel.Salamon-at-nrc-cnrc.gc.ca Wed Feb 8 15:33:09 2006
5, 23 -- Received: from nrccenexf2.nrc.ca (nrccenexf2.nrc.ca [132.246.15.83])
5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k18LX8mG009977
5, 23 -- for {microscopy-at-microscopy.com} ; Wed, 8 Feb 2006 15:33:08 -0600
5, 23 -- Received: from nrccenexb2.nrc.ca ([132.246.15.98]) by nrccenexf2.nrc.ca with Microsoft SMTPSVC(6.0.3790.1830);
5, 23 -- Wed, 8 Feb 2006 16:33:07 -0500
5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
5, 23 -- Content-class: urn:content-classes:message
5, 23 -- MIME-Version: 1.0
5, 23 -- Content-Type: text/plain;
5, 23 -- charset="us-ascii"
5, 23 -- Subject: S4800 STEM scintillator size
5, 23 -- Date: Wed, 8 Feb 2006 16:32:19 -0500
5, 23 -- Message-ID: {923687253229634E96E808B8D3124C83557517-at-nrccenexb2.nrc.ca}
5, 23 -- X-MS-Has-Attach:
5, 23 -- X-MS-TNEF-Correlator:
5, 23 -- Thread-Topic: S4800 STEM scintillator size
5, 23 -- Thread-Index: AcYs9yOtLSo8zGW/Rh2wOcOIwp9aqA==
5, 23 -- From: "Salamon, Daniel" {Daniel.Salamon-at-nrc-cnrc.gc.ca}
5, 23 -- To: {microscopy-at-microscopy.com}
5, 23 -- X-OriginalArrivalTime: 08 Feb 2006 21:33:07.0447 (UTC) FILETIME=[4054A070:01C62CF7]
5, 23 -- Content-Transfer-Encoding: 8bit
5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k18LX8mG009977
==============================End of - Headers==============================




From: gerd.leitinger-at-meduni-graz.at
Date: Thu, 9 Feb 2006 06:45:00 -0600
Subject: [Microscopy] EM: precautions when en bloc staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear list,

We are just starting an experiment with en-bloc staining of tissue in uranyl
acetate in 70% ethanol.
It seems to me that since the blocks are larger than thin sections, they
probably take up more radioactive material- so should we take any
precautions when handling the blocks after the resin has cured? (i.e. store
the blocks in separate special containers, wear gloves when sectioning or
are there any special cleaning procedures for the knife?).

thank you for sharing your knowledge

Gerd

Dr. Gerd Leitinger

Institut für Zellbiologie, Histologie und Embryologie
Medizinische Universität Graz
Harrachgasse 21
A-8010 Graz
Austria

Tel. ++43 316 380 4237
Fax. ++43 316 380 9625
Mailto: Gerd.Leitinger-at-meduni-graz.at



==============================Original Headers==============================
8, 19 -- From gerd.leitinger-at-meduni-graz.at Thu Feb 9 06:45:00 2006
8, 19 -- Received: from herakles.kfunigraz.ac.at (HERAKLES.kfunigraz.ac.at [143.50.13.36])
8, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19CixHm007152
8, 19 -- for {microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 06:45:00 -0600
8, 19 -- Received: from [10.200.112.73] ([193.170.104.185]) by herakles.kfunigraz.ac.at with Microsoft SMTPSVC(5.0.2195.5329);
8, 19 -- Thu, 9 Feb 2006 13:44:54 +0100
8, 19 -- From: "Gerd Leitinger" {gerd.leitinger-at-meduni-graz.at}
8, 19 -- To: microscopy-at-microscopy.com
8, 19 -- Date: Thu, 09 Feb 2006 13:40:39 +0100
8, 19 -- MIME-Version: 1.0
8, 19 -- Subject: EM: precautions when en bloc staining
8, 19 -- Message-ID: {43EB4657.20348.F46BF7-at-gerd.leitinger.meduni-graz.at}
8, 19 -- Priority: normal
8, 19 -- X-mailer: Pegasus Mail for Windows (4.31, DE v4.31 R1)
8, 19 -- Content-type: text/plain; charset=ISO-8859-1
8, 19 -- Content-description: Mail message body
8, 19 -- X-OriginalArrivalTime: 09 Feb 2006 12:44:54.0222 (UTC) FILETIME=[A01C06E0:01C62D76]
8, 19 -- Content-Transfer-Encoding: 8bit
8, 19 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id k19CixHm007152
==============================End of - Headers==============================




From: donc-at-asmicro.com
Date: Thu, 9 Feb 2006 06:56:12 -0600
Subject: [Microscopy] AFM equipment wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I am seeking to buy NanoScope AFM equipment, such as MultiMode or Dimension,
to use with my Nanoscope IIIA controller. If you have anything to sell,
please contact me offline.

regards,
Don Chernoff
==================================
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]


==============================Original Headers==============================
3, 21 -- From donc-at-asmicro.com Thu Feb 9 06:56:12 2006
3, 21 -- Received: from smtp112.sbc.mail.re2.yahoo.com (smtp112.sbc.mail.re2.yahoo.com [68.142.229.93])
3, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k19CuBAg016578
3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 06:56:11 -0600
3, 21 -- Received: (qmail 34978 invoked from network); 9 Feb 2006 12:56:11 -0000
3, 21 -- Received: from unknown (HELO ASM11) (asmicro-at-sbcglobal.net-at-68.57.205.80 with login)
3, 21 -- by smtp112.sbc.mail.re2.yahoo.com with SMTP; 9 Feb 2006 12:56:11 -0000
3, 21 -- Message-ID: {006801c62d77$ebd28b80$c901a8c0-at-ASM11}
3, 21 -- Reply-To: "Don Chernoff at ASM" {donc-at-asmicro.com}
3, 21 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com}
3, 21 -- To: "Microscopy List" {microscopy-at-microscopy.com}
3, 21 -- Subject: AFM equipment wanted
3, 21 -- Date: Thu, 9 Feb 2006 07:54:03 -0500
3, 21 -- MIME-Version: 1.0
3, 21 -- Content-Type: text/plain;
3, 21 -- charset="iso-8859-1"
3, 21 -- Content-Transfer-Encoding: 7bit
3, 21 -- X-Priority: 3
3, 21 -- X-MSMail-Priority: Normal
3, 21 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1506
3, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1506
==============================End of - Headers==============================




From: cumings-at-umd.edu
Date: Thu, 9 Feb 2006 08:35:58 -0600
Subject: [Microscopy] Wanted: Gatan 622SC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello microscopists,

We are looking to purchase a second-hand Gatan 622SC camera
(200kV-compatible) in decent working condition to install on our JEOL
2100 TEM. We are hoping to find one with a working intensifier and an
intact scintillator, although any problems are possibly acceptable.
We would be able to pay for it using a University of Maryland PO. The
amount of money might allow you to upgrade your TEM to a digital CCD
camera from AMT.

Please do not reply to the listserver. Direct any inquiries or offers
directly to me at the email address below.

Many thanks,

-John

--
John Cumings
cumings-at-umd.edu
Assistant Professor
Department of Materials Science and Engineering
University of Maryland
College Park, MD 20742-2115

office (301) 405-0789 (1246 Kim Building)
fax (301) 314-8164
--


==============================Original Headers==============================
8, 25 -- From jcumings-at-gmail.com Thu Feb 9 08:35:58 2006
8, 25 -- Received: from uproxy.gmail.com (uproxy.gmail.com [66.249.92.204])
8, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19EZv1W027008
8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 08:35:58 -0600
8, 25 -- Received: by uproxy.gmail.com with SMTP id s2so260073uge
8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 09 Feb 2006 06:35:57 -0800 (PST)
8, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
8, 25 -- s=beta; d=gmail.com;
8, 25 -- h=received:message-id:date:from:reply-to:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition;
8, 25 -- b=J7Yh3j/yK8qwQ23qXD6S/Pwrua8D74E3rIXGt9kBx4eFw1tKTqNnPy7smG5lUD3ppuvFgteak9KXbxsNCbt+yyJ4pxWsBJTngWRcNa97f2Faq+nWy5TFTU80zgJW5XqVm16M9Jh1a1he3KQXpbV+6SwDYRIc7trYXy9XsKUAFs0=
8, 25 -- Received: by 10.48.223.10 with SMTP id v10mr796858nfg;
8, 25 -- Thu, 09 Feb 2006 06:35:56 -0800 (PST)
8, 25 -- Received: by 10.49.55.19 with HTTP; Thu, 9 Feb 2006 06:35:56 -0800 (PST)
8, 25 -- Message-ID: {c4e021530602090635k847eadej4ffd50ec558f97c0-at-mail.gmail.com}
8, 25 -- Date: Thu, 9 Feb 2006 09:35:56 -0500
8, 25 -- From: John Cumings {cumings-at-umd.edu}
8, 25 -- Reply-To: cumings-at-umd.edu
8, 25 -- Sender: jcumings-at-gmail.com
8, 25 -- To: Microscopy-at-microscopy.com
8, 25 -- Subject: Wanted: Gatan 622SC
8, 25 -- MIME-Version: 1.0
8, 25 -- Content-Type: text/plain; charset=ISO-8859-1
8, 25 -- Content-Disposition: inline
8, 25 -- Content-Transfer-Encoding: 8bit
8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k19EZv1W027008
==============================End of - Headers==============================




From: hyi-at-emory.edu
Date: Thu, 9 Feb 2006 10:06:53 -0600
Subject: [Microscopy] Bacterial Pili

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Microscopists:


We have a few users study bacterial pili using negative staining with
PTA (pH 6.5). We have got some pretty nice images. But often time, we
could not find pili even on the positive control.  When we did see
pili, they were not on all bacteria in the same sample. Is this a
common phenomenon? Do bacteria lose their pili easily when the external
condition is not favorable? if that is the case, what should be done to
minimize the loss.

Thank you in advance. 

Hong
Emory EM


==============================Original Headers==============================
5, 22 -- From hyi-at-emory.edu Thu Feb 9 10:06:53 2006
5, 22 -- Received: from virginia.cc.emory.edu (virginia.cc.emory.edu [170.140.8.222])
5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19G6qLb005145
5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 10:06:53 -0600
5, 22 -- Received: from [170.140.232.204] (localhost [127.0.0.1])
5, 22 -- by virginia.cc.emory.edu (8.13.4/8.13.4) with ESMTP id k19G6qIx024009
5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 11:06:52 -0500 (EST)
5, 22 -- Mime-Version: 1.0 (Apple Message framework v622)
5, 22 -- Message-Id: {56be70af163d5629d409dc9e17804801-at-emory.edu}
5, 22 -- Content-Type: text/plain;
5, 22 -- charset=ISO-8859-1;
5, 22 -- format=flowed
5, 22 -- To: Microscopy-at-microscopy.com
5, 22 -- From: Hong Yi {hyi-at-emory.edu}
5, 22 -- Subject: Bacterial Pili
5, 22 -- Date: Thu, 9 Feb 2006 11:06:51 -0500
5, 22 -- X-Mailer: Apple Mail (2.622)
5, 22 -- X-imss-version: 2.037
5, 22 -- X-imss-result: Passed
5, 22 -- X-imss-approveListMatch: *-at-emory.edu
5, 22 -- Content-Transfer-Encoding: 8bit
5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k19G6qLb005145
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Thu, 9 Feb 2006 10:24:23 -0600
Subject: [Microscopy] Bacterial Pili

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hong,

We often have this same problem. At least in our lab, flagella and pili seem to hide when we're trying to find them and show up when we don't care if we see them or not.

As a rule, they seem to be easily detached by rough handling, including centrifuging, and maybe by the staining process itself, since we often find them isolated from the bacteria on the grid.

One group of researchers gets around this problem by growing the bacteria on carbon coated grids and staining them in situ. Check JOURNAL OF BACTERIOLOGY, Feb. 2005, p. 1173-1181 for images. For preparation details this article references Brown et al, 2001. Immunocytochemical localization of HrpA and HrpZ supports a role for the Hrp pilus in the transfer of effector proteins from Pseudomonassyringae pv. tomato across the host plant cell wall. Mol. Plant-Microbe Interact. 14:394-404. I have not yet read the last article, so I can't comment on it.

Good luck.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







-----Original Message-----
X-from: hyi-at-emory.edu [mailto:hyi-at-emory.edu]
Sent: Thursday, February 09, 2006 10:08 AM
To: Tindall, Randy D.

Dear Microscopists:


We have a few users study bacterial pili using negative staining with PTA (pH 6.5). We have got some pretty nice images. But often time, we could not find pili even on the positive control.  When we did see pili, they were not on all bacteria in the same sample. Is this a common phenomenon? Do bacteria lose their pili easily when the external condition is not favorable? if that is the case, what should be done to minimize the loss.

Thank you in advance. 

Hong
Emory EM


==============================Original Headers==============================
5, 22 -- From hyi-at-emory.edu Thu Feb 9 10:06:53 2006 5, 22 -- Received: from virginia.cc.emory.edu (virginia.cc.emory.edu [170.140.8.222])
5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19G6qLb005145
5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 10:06:53 -0600
5, 22 -- Received: from [170.140.232.204] (localhost [127.0.0.1])
5, 22 -- by virginia.cc.emory.edu (8.13.4/8.13.4) with ESMTP id k19G6qIx024009
5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 11:06:52 -0500 (EST)
5, 22 -- Mime-Version: 1.0 (Apple Message framework v622) 5, 22 -- Message-Id: {56be70af163d5629d409dc9e17804801-at-emory.edu}
5, 22 -- Content-Type: text/plain;
5, 22 -- charset=ISO-8859-1;
5, 22 -- format=flowed
5, 22 -- To: Microscopy-at-microscopy.com
5, 22 -- From: Hong Yi {hyi-at-emory.edu}
5, 22 -- Subject: Bacterial Pili
5, 22 -- Date: Thu, 9 Feb 2006 11:06:51 -0500 5, 22 -- X-Mailer: Apple Mail (2.622) 5, 22 -- X-imss-version: 2.037 5, 22 -- X-imss-result: Passed 5, 22 -- X-imss-approveListMatch: *-at-emory.edu 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k19G6qLb005145 ==============================End of - Headers==============================


==============================Original Headers==============================
20, 24 -- From TindallR-at-missouri.edu Thu Feb 9 10:24:23 2006
20, 24 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49])
20, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19GOMnC014637
20, 24 -- for {microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 10:24:22 -0600
20, 24 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830);
20, 24 -- Thu, 9 Feb 2006 10:24:22 -0600
20, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
20, 24 -- Content-class: urn:content-classes:message
20, 24 -- MIME-Version: 1.0
20, 24 -- Content-Type: text/plain;
20, 24 -- charset="iso-8859-1"
20, 24 -- Subject: RE: [Microscopy] Bacterial Pili
20, 24 -- Date: Thu, 9 Feb 2006 10:24:21 -0600
20, 24 -- Message-ID: {BA876152E8653240BE8572E897083EE701849D33-at-UM-EMAIL09.um.umsystem.edu}
20, 24 -- X-MS-Has-Attach:
20, 24 -- X-MS-TNEF-Correlator:
20, 24 -- Thread-Topic: [Microscopy] Bacterial Pili
20, 24 -- thread-index: AcYtkwuBClshBOD6QsCkENhoqgFICQAAJ20Q
20, 24 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
20, 24 -- To: {hyi-at-emory.edu}
20, 24 -- Cc: {microscopy-at-microscopy.com}
20, 24 -- X-OriginalArrivalTime: 09 Feb 2006 16:24:22.0502 (UTC) FILETIME=[49041860:01C62D95]
20, 24 -- Content-Transfer-Encoding: 8bit
20, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k19GOMnC014637
==============================End of - Headers==============================




From: Edward.Calomeni-at-osumc.edu
Date: Thu, 9 Feb 2006 11:18:04 -0600
Subject: [Microscopy] Bacterial Pili

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hong,

Rough handling may contribute to loss of pili, however the PTA itself is
probably the culprit. Try changing the pH either up or down. Way back when,
I did a study with rotavirus and various negative stains. Bottom line is
that the length of time of staining, pH and concentration all contributed to
the destruction (preservation) of the viral particles. If I remember
correctly, the length of time was the most important factor. Also try using
either uranyl acetate or ammonium molybdate as the negative stain.

Best of luck,

Ed


Edward P. Calomeni
Director EM Lab - Pathology
The Ohio State University
M018 Starling Loving Hall
320 W. 10th Ave.
Columbus, OH 43210
614-293-5580 (office)
614-293-8806 (lab)
edward.calomeni-at-osumc.edu
-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: Thursday, February 09, 2006 11:29 AM
To: Edward Calomeni

Hong,

We often have this same problem. At least in our lab, flagella and pili seem
to hide when we're trying to find them and show up when we don't care if we
see them or not.

As a rule, they seem to be easily detached by rough handling, including
centrifuging, and maybe by the staining process itself, since we often find
them isolated from the bacteria on the grid.

One group of researchers gets around this problem by growing the bacteria on
carbon coated grids and staining them in situ. Check JOURNAL OF
BACTERIOLOGY, Feb. 2005, p. 1173-1181 for images. For preparation details
this article references Brown et al, 2001. Immunocytochemical localization
of HrpA and HrpZ supports a role for the Hrp pilus in the transfer of
effector proteins from Pseudomonassyringae pv. tomato across the host plant
cell wall. Mol. Plant-Microbe Interact. 14:394-404. I have not yet read the
last article, so I can't comment on it.

Good luck.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







-----Original Message-----
X-from: hyi-at-emory.edu [mailto:hyi-at-emory.edu]
Sent: Thursday, February 09, 2006 10:08 AM
To: Tindall, Randy D.

Dear Microscopists:


We have a few users study bacterial pili using negative staining with
PTA (pH 6.5). We have got some pretty nice images. But often time, we could
not find pili even on the positive control.  When we did see pili, they were
not on all bacteria in the same sample. Is this a common phenomenon? Do
bacteria lose their pili easily when the external condition is not
favorable? if that is the case, what should be done to minimize the loss.

Thank you in advance. 

Hong
Emory EM


==============================Original Headers==============================
5, 22 -- From hyi-at-emory.edu Thu Feb 9 10:06:53 2006 5, 22 -- Received: from
virginia.cc.emory.edu (virginia.cc.emory.edu [170.140.8.222])
5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
k19G6qLb005145
5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 10:06:53
-0600
5, 22 -- Received: from [170.140.232.204] (localhost [127.0.0.1])
5, 22 -- by virginia.cc.emory.edu (8.13.4/8.13.4) with ESMTP id
k19G6qIx024009
5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 11:06:52
-0500 (EST)
5, 22 -- Mime-Version: 1.0 (Apple Message framework v622) 5, 22 --
Message-Id: {56be70af163d5629d409dc9e17804801-at-emory.edu}
5, 22 -- Content-Type: text/plain;
5, 22 -- charset=ISO-8859-1;
5, 22 -- format=flowed
5, 22 -- To: Microscopy-at-microscopy.com
5, 22 -- From: Hong Yi {hyi-at-emory.edu}
5, 22 -- Subject: Bacterial Pili
5, 22 -- Date: Thu, 9 Feb 2006 11:06:51 -0500 5, 22 -- X-Mailer: Apple Mail
(2.622) 5, 22 -- X-imss-version: 2.037 5, 22 -- X-imss-result: Passed 5, 22
-- X-imss-approveListMatch: *-at-emory.edu 5, 22 -- Content-Transfer-Encoding:
8bit 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id k19G6qLb005145 ==============================End of -
Headers==============================


==============================Original Headers==============================
20, 24 -- From TindallR-at-missouri.edu Thu Feb 9 10:24:23 2006
20, 24 -- Received: from um-exproto9.um.umsystem.edu
(um-exproto9.um.umsystem.edu [207.160.151.49])
20, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
k19GOMnC014637
20, 24 -- for {microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 10:24:22
-0600
20, 24 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by
um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830);
20, 24 -- Thu, 9 Feb 2006 10:24:22 -0600
20, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
20, 24 -- Content-class: urn:content-classes:message
20, 24 -- MIME-Version: 1.0
20, 24 -- Content-Type: text/plain;
20, 24 -- charset="iso-8859-1"
20, 24 -- Subject: RE: [Microscopy] Bacterial Pili
20, 24 -- Date: Thu, 9 Feb 2006 10:24:21 -0600
20, 24 -- Message-ID:
{BA876152E8653240BE8572E897083EE701849D33-at-UM-EMAIL09.um.umsystem.edu}
20, 24 -- X-MS-Has-Attach:
20, 24 -- X-MS-TNEF-Correlator:
20, 24 -- Thread-Topic: [Microscopy] Bacterial Pili
20, 24 -- thread-index: AcYtkwuBClshBOD6QsCkENhoqgFICQAAJ20Q
20, 24 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
20, 24 -- To: {hyi-at-emory.edu}
20, 24 -- Cc: {microscopy-at-microscopy.com}
20, 24 -- X-OriginalArrivalTime: 09 Feb 2006 16:24:22.0502 (UTC)
FILETIME=[49041860:01C62D95]
20, 24 -- Content-Transfer-Encoding: 8bit
20, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id k19GOMnC014637
==============================End of - Headers==============================


==============================Original Headers==============================
31, 31 -- From Edward.Calomeni-at-osumc.edu Thu Feb 9 11:18:04 2006
31, 31 -- Received: from pluto.osumc.edu (pluto.osumc.edu [140.254.120.27])
31, 31 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19HI3nX024586
31, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 11:18:03 -0600
31, 31 -- Received: from localhost (unknown [127.0.0.1])
31, 31 -- by pfeg02.osumc.edu (Postfix) with ESMTP id 01EE2E22B
31, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 12:18:04 -0500 (EST)
31, 31 -- Received: from pfeg02.osumc.edu ([127.0.0.1])
31, 31 -- by localhost (pfeg02 [127.0.0.1]) (amavisd-new, port 10024) with LMTP
31, 31 -- id 04255-01-25 for {Microscopy-at-microscopy.com} ;
31, 31 -- Thu, 9 Feb 2006 17:18:03 +0000 (GMT)
31, 31 -- Received: from msxc01.OSUMC.EDU (unknown [10.127.29.33])
31, 31 -- by pfeg02.osumc.edu (Postfix) with ESMTP id D2A8BE03F
31, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 12:18:03 -0500 (EST)
31, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0
31, 31 -- Content-class: urn:content-classes:message
31, 31 -- MIME-Version: 1.0
31, 31 -- Content-Type: text/plain;
31, 31 -- charset="iso-8859-1"
31, 31 -- Subject: RE: Bacterial Pili
31, 31 -- Date: Thu, 9 Feb 2006 12:18:03 -0500
31, 31 -- Message-ID: {7A541592F9A53A4E86E7A3D5C4C7F68C27F9F4-at-MSXC01}
31, 31 -- X-MS-Has-Attach:
31, 31 -- X-MS-TNEF-Correlator:
31, 31 -- Thread-Topic: RE: Bacterial Pili
31, 31 -- Thread-Index: AcYtleiHrKzoLX0fRmSx0dkDmP6AHwABibyw
31, 31 -- From: "Edward Calomeni" {Edward.Calomeni-at-osumc.edu}
31, 31 -- To: {Microscopy-at-microscopy.com}
31, 31 -- X-Virus-Scanned: amavisd-new at osumc.edu
31, 31 -- Content-Transfer-Encoding: 8bit
31, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k19HI3nX024586
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Thu, 9 Feb 2006 12:48:20 -0600
Subject: [Microscopy] Re: EM: precautions when en bloc staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Feb 9, 2006, at 4:45 AM, gerd.leitinger-at-meduni-graz.at wrote:

} We are just starting an experiment with en-bloc staining of tissue in
} uranyl
} acetate in 70% ethanol.
} It seems to me that since the blocks are larger than thin sections,
} they
} probably take up more radioactive material- so should we take any
} precautions when handling the blocks after the resin has cured? (i.e.
} store
} the blocks in separate special containers, wear gloves when sectioning
} or
} are there any special cleaning procedures for the knife?).
}
} thank you for sharing your knowledge
}
Dear Gerd,
There is still a very small amount of radioactive material in the
block; furthermore, uranium has a very long half-life, thus very small
activity, and the alpha particles it emits have a short range, so any
decays except those nearer the surface of the block than the thickness
of the dead layer of your skin will not result in much radiation
leaving the block. You can easily measure this with an ionization
chamber (to detect the x- and gamma-radiation that make up most of the
escaping radiation). That said, the laws regarding the handling of
radioactive materials--even those with very little activity--vary from
country to country, locale to locale, and sometimes for different
institutions within a particular locale, so your safety office may
mandate special procedures for storage and handling.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
4, 22 -- From tivol-at-caltech.edu Thu Feb 9 12:48:19 2006
4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19ImIdP002791
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Thu, 9 Feb 2006 12:48:19 -0600
4, 22 -- Received: from localhost (water-dog [192.168.1.26])
4, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 33AB23422E
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Thu, 9 Feb 2006 10:48:18 -0800 (PST)
4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21])
4, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id 6844F33A1E
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Thu, 9 Feb 2006 10:48:17 -0800 (PST)
4, 22 -- Mime-Version: 1.0 (Apple Message framework v623)
4, 22 -- In-Reply-To: {200602091245.k19Cj91Q007324-at-ns.microscopy.com}
4, 22 -- References: {200602091245.k19Cj91Q007324-at-ns.microscopy.com}
4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
4, 22 -- Message-Id: {9fac5de4ab7271490d0c8faa23e1db2b-at-caltech.edu}
4, 22 -- Content-Transfer-Encoding: 7bit
4, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
4, 22 -- Subject: Re: [Microscopy] EM: precautions when en bloc staining
4, 22 -- Date: Thu, 9 Feb 2006 10:55:56 -0800
4, 22 -- To: microscopy-at-msa.microscopy.com
4, 22 -- X-Mailer: Apple Mail (2.623)
4, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.3.3
==============================End of - Headers==============================




From: nairvinods-at-gmail.com
Date: Thu, 9 Feb 2006 13:08:22 -0600
Subject: [Microscopy] Re: Bacterial Pili

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Hong,
I have been using 2% Uranyl acetate to visualize bacterial pili.

I have been using 2% Uranyl acetate to visualize pili. I have had to
play with the time of staining and rinsing in order to get a good
contrast to visualize the pili on different bacterial genera. I have
noticed that not all bacteria show the same distribution of pili.
I have also noticed that the way you grow the bacteria also affect
visualization of pili. For our bacteria I have noticed that I get far
better results when I grow them up in stationary cultures without
shaking them.

What I have been seeing it the bacteria behave differently when in
aggregation versus solitude. This is more so owing to the several
differnt kinds of pili each bacterial strain is capable of forming. If
the bacteria you are working with produces different types of pili
then you will see differences between the bacterial cells visualized
on the same grid.

I have had the same strain of bacteria producing really small pili
which were visualized only at a very high mag.
Hope that was helpful
regards,
Vinod
Graduate Student
Dept Of Biology
New Mexico State University
On 2/9/06, hyi-at-emory.edu {hyi-at-emory.edu} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Microscopists:
}
}
} We have a few users study bacterial pili using negative staining with
} PTA (pH 6.5). We have got some pretty nice images. But often time, we
} could not find pili even on the positive control. When we did see
} pili, they were not on all bacteria in the same sample. Is this a
} common phenomenon? Do bacteria lose their pili easily when the external
} condition is not favorable? if that is the case, what should be done to
} minimize the loss.
}
} Thank you in advance.
}
} Hong
} Emory EM
}
}
} ==============================Original Headers==============================
} 5, 22 -- From hyi-at-emory.edu Thu Feb 9 10:06:53 2006
} 5, 22 -- Received: from virginia.cc.emory.edu (virginia.cc.emory.edu
} [170.140.8.222])
} 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19G6qLb005145
} 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 10:06:53 -0600
} 5, 22 -- Received: from [170.140.232.204] (localhost [127.0.0.1])
} 5, 22 -- by virginia.cc.emory.edu (8.13.4/8.13.4) with ESMTP id
} k19G6qIx024009
} 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 11:06:52 -0500
} (EST)
} 5, 22 -- Mime-Version: 1.0 (Apple Message framework v622)
} 5, 22 -- Message-Id: {56be70af163d5629d409dc9e17804801-at-emory.edu}
} 5, 22 -- Content-Type: text/plain;
} 5, 22 -- charset=ISO-8859-1;
} 5, 22 -- format=flowed
} 5, 22 -- To: Microscopy-at-microscopy.com
} 5, 22 -- From: Hong Yi {hyi-at-emory.edu}
} 5, 22 -- Subject: Bacterial Pili
} 5, 22 -- Date: Thu, 9 Feb 2006 11:06:51 -0500
} 5, 22 -- X-Mailer: Apple Mail (2.622)
} 5, 22 -- X-imss-version: 2.037
} 5, 22 -- X-imss-result: Passed
} 5, 22 -- X-imss-approveListMatch: *-at-emory.edu
} 5, 22 -- Content-Transfer-Encoding: 8bit
} 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id k19G6qLb005145
} ==============================End of - Headers==============================
}


==============================Original Headers==============================
5, 25 -- From nairvinods-at-gmail.com Thu Feb 9 13:08:22 2006
5, 25 -- Received: from wproxy.gmail.com (wproxy.gmail.com [64.233.184.202])
5, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19J8Mrd012527
5, 25 -- for {microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 13:08:22 -0600
5, 25 -- Received: by wproxy.gmail.com with SMTP id i6so371281wra
5, 25 -- for {microscopy-at-microscopy.com} ; Thu, 09 Feb 2006 11:08:22 -0800 (PST)
5, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
5, 25 -- s=beta; d=gmail.com;
5, 25 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references;
5, 25 -- b=EaYibLaG48bwxRHn2D1rKg1YmKGyCmDnLAqNF+/f9UuSlcSOnBwQBCRPkBMXnmuuxJwTWlUo0r2HcibDDb78jLOk2qjdWIZ4b+Fwteoxowx/jA8BP9NFOLKwkpPzMMYrvgo4Ba23mGe3Ujr2MYVW72FvurUNj517iGySHrdgce8=
5, 25 -- Received: by 10.65.180.18 with SMTP id h18mr161301qbp;
5, 25 -- Thu, 09 Feb 2006 11:08:21 -0800 (PST)
5, 25 -- Received: by 10.64.203.13 with HTTP; Thu, 9 Feb 2006 11:08:21 -0800 (PST)
5, 25 -- Message-ID: {ea42a3900602091108occ4907aw88fdbc39652af5a0-at-mail.gmail.com}
5, 25 -- Date: Thu, 9 Feb 2006 12:08:21 -0700
5, 25 -- From: Vinod Nair {nairvinods-at-gmail.com}
5, 25 -- To: microscopy-at-microscopy.com
5, 25 -- Subject: Re: Bacterial Pili
5, 25 -- In-Reply-To: {200602091612.k19GC6Bt012799-at-ns.microscopy.com}
5, 25 -- MIME-Version: 1.0
5, 25 -- Content-Type: text/plain; charset=UTF-8
5, 25 -- Content-Disposition: inline
5, 25 -- References: {200602091612.k19GC6Bt012799-at-ns.microscopy.com}
5, 25 -- Content-Transfer-Encoding: 8bit
5, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id k19J8Mrd012527
==============================End of - Headers==============================




From: icmicroanalysis-at-cox.net
Date: Thu, 9 Feb 2006 13:09:33 -0600
Subject: [Microscopy] Stains/etches for delineating source/drains diffusions on semiconductor die cross sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I'm compiling a list of chemical stains/etches for delineating N+ and P+
source/drain implants/diffusions on semiconductor die cross sections. Any
suggestions?


==============================Original Headers==============================
3, 22 -- From icmicroanalysis-at-cox.net Thu Feb 9 13:09:32 2006
3, 22 -- Received: from fed1rmmtao03.cox.net (fed1rmmtao03.cox.net [68.230.241.36])
3, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19J9WI4014727
3, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 13:09:32 -0600
3, 22 -- Received: from officehp1 ([68.98.17.129]) by fed1rmmtao03.cox.net
3, 22 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with SMTP
3, 22 -- id {20060209190811.LEDO20875.fed1rmmtao03.cox.net-at-officehp1}
3, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 14:08:11 -0500
3, 22 -- From: "IC Microanalysis" {icmicroanalysis-at-cox.net}
3, 22 -- To: {Microscopy-at-microscopy.com}
3, 22 -- Subject: Stains/etches for delineating source/drains diffusions on semiconductor die cross sections
3, 22 -- Date: Thu, 9 Feb 2006 12:09:30 -0700
3, 22 -- Message-ID: {IKEHJCMBLNGNPJBGCPGJIEKACCAA.icmicroanalysis-at-cox.net}
3, 22 -- MIME-Version: 1.0
3, 22 -- Content-Type: text/plain;
3, 22 -- charset="iso-8859-1"
3, 22 -- Content-Transfer-Encoding: 7bit
3, 22 -- X-Priority: 3 (Normal)
3, 22 -- X-MSMail-Priority: Normal
3, 22 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2910.0)
3, 22 -- Importance: Normal
3, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
==============================End of - Headers==============================




From: hagglundk1-at-nku.edu
Date: Fri, 10 Feb 2006 10:11:00 -0600
Subject: [Microscopy] Follow up ESEM gas choices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I put together a summary of the responses to my query on ESEM gas
choices. People responded that they had experimented with Nitrogen,
Nitrous Oxide, Oxygen, and Helium. Opinions were mixed on the
performance of various gases (see below), but in general I feel
comfortable trying Nitrogen in the near future. I received warnings
that Helium can damage photomultipliers and Argon can damage ion pumps.
Because of safety issues, we will most likely avoid oxygen and nitrous
oxide for the time being.

FEI also contacted me and reminded that before experimenting with
various gases in the ESEM, I should check with them to make sure that
warranties are not voided by using any particular gas. If a gas could
damage your system or components, the manufacturer should be able to
tell you before you damage your system. Always check with the
manufacturer before changing major system parameters.

My detector issue drew a number of separate questions and queries, and
we are working separately with the manufacturers on this. We did learn
that we can collect spectra with the SDD crystal at room temperature.
This eliminates the potential for vapor condensation on the crystal
surface. There is some peak broadening and a slightly higher background
when we do this, but it does provide us with bulk data and may prove to
be our final solution.

I also learned that many bulk gases also contain amounts of moisture
that might condense under variable pressure conditions. Ultra high
purity, low moisture gas, is sometimes considerably more expensive than
bulk lab gas. I have to check with my vendor to find out what kind of
moisture my tank might contain before hooking it to the ESEM.

Thanks again for everyone's help on this. The list has proven itself
invaluable again.

Karl

-----
"I have used water vapor, N2 and Argon. None of them gave as good a
signal (image) as the water vapor did.... The Ionization of the other
gasses is apparently not that good and give no nice images"
-----
"I have been using dry nitrogen with my variable pressure instrument. We
vent the system with the same gas. My EDS system seems to work well but
have not run extensive tests to determine what effects the gas has other
than the beam scattering issues."
-----
" A disadvantage to using helium is your PMT's could be damaged, if it
is released into the room. Also, another gas you could be careful with
is argon, as it is a ion-pump poison (if released into a ion-pumped
system).
Nitrogen sounds friendly to me"
-----
"In my experience, if you can't use oxygen the next best gas to use is
argon - easily obtainable and provides good imaging conditions. Air and
nitrogen should be avoided, since the nitrogen breaks down at too low a
voltage and you can't get much signal. Some European groups looked at a
wider range of imaging gases and nitrous oxide I believe did very well,
although I have not tried it. I have tried oxygen and it works well, but
tends to ruin the pump oil fairly quickly."
-----
"The Quanta 200 uses a W filament. Unless it is a SFEG. If there are
no ion pumps, you should be able to use Nitrogen or He. If there is an
ion pump, do not use He. It will kill the pump.

I found that for VP (20-120Pa) that N2 works fine and is better than air
(less vacuum issues). So, for ESEM, I would think that N2 ought to be
the gas of choice as well."
-----
" We routinely use He in our Hitachi VP-SEM. It has a tungsten gun and
no ion pumps, so there is no problem with fouling those pumps as Gary
Gaugler mentioned.

If you consider that He is a monoatomic species with a weight of 4 and
that nitrogen is diatomic with a weight of 28, your gut can tell you
that He will scatter less than N2 or room air. That is our experience
and we can see the effect in iamges.

There is no effect on the x-ray signals from He that we have ever seen.
He x-rays are below our low-energy discriminator. I suppose one could
see N or O x-rays due to the gas, but I suppose their contribution is
much less than from the sample. You could arrange a test to evaluate
that, say you collected spectra from a beryllium or boron sample using
high vacuum, helium, and nitrogen."
-----
Original Post:
---
----

I am interested in hearing what type of gases people are using in their
ESEM applications. We recently learned our silicon drift detector is
incompatible with water vapor, and we apparently cannot complete any
x-ray microanalysis while working in standard ESEM or VP modes (I was
pretty surprised to learn this). We are thinking that using a dry gas
such as nitrogen or helium will allow us to work in ESEM modes and use
our x-ray system as well, as there is no potential for moisture
condensing on the crystal surface.

Our ESEM is a Quanta 200, and it is not equipped with the peltier stage,
so we mainly use the environmental modes to alleviate charging in
uncoated samples. The x-ray system was purchased with the microscope
about four years ago. I would rather not discuss the name of the x-ray
vendor on the list, as we have a significant investment in this detector
and do not foresee finding the money to purchase a new one soon.

Our first concern is the impact of the gas on spectra. We can coat
samples and run them in high vacuum mode, but customers like spectra
that represent the sample and not the coating material. We periodically
have samples that cannot be coated, so ESEM becomes critical for our
imaging needs. If the gas is going to have a dramatic impact on signal,
are we better off coating and running high vacuum?

Is there a best choice for chamber gas? Our service engineer recommends
nitrogen as having benefits for keeping the system clean. We can set it
up fairly quickly, and I have a spare tank and regulator. I have heard
of people using helium and believe that I read somewhere that there was
some advantage to using helium.

I am still trying to get information from the vendor on whether this
will allow us to use the x-ray and ESEM simultaneously, or whether we
have options with different collimators or windows. Their responses
have been pretty slow coming.
_____

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu



==============================Original Headers==============================
18, 23 -- From hagglundk1-at-nku.edu Fri Feb 10 10:10:59 2006
18, 23 -- Received: from mailfe2.nku.edu (exchange.nku.edu [192.122.237.68])
18, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1AGAxYh024896
18, 23 -- for {microscopy-at-microscopy.com} ; Fri, 10 Feb 2006 10:10:59 -0600
18, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211);
18, 23 -- Fri, 10 Feb 2006 11:10:59 -0500
18, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0
18, 23 -- Content-class: urn:content-classes:message
18, 23 -- MIME-Version: 1.0
18, 23 -- Content-Type: text/plain;
18, 23 -- charset="us-ascii"
18, 23 -- Subject: Follow up ESEM gas choices
18, 23 -- Date: Fri, 10 Feb 2006 11:10:56 -0500
18, 23 -- Message-ID: {F01E4499C4EC5842A8AB7198141AC72F358647-at-mailfac1.hh.nku.edu}
18, 23 -- X-MS-Has-Attach:
18, 23 -- X-MS-TNEF-Correlator:
18, 23 -- Thread-Topic: Follow up ESEM gas choices
18, 23 -- Thread-Index: AcYuXJL+d7H9dmpuRdS+OZyc5Rw51w==
18, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu}
18, 23 -- To: {microscopy-at-microscopy.com}
18, 23 -- X-OriginalArrivalTime: 10 Feb 2006 16:10:59.0208 (UTC) FILETIME=[94A0E880:01C62E5C]
18, 23 -- Content-Transfer-Encoding: 8bit
18, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1AGAxYh024896
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Fri, 10 Feb 2006 10:20:25 -0600
Subject: [Microscopy] S4800 STEM scintillator size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Daniel,
If you contact Gene Taylor at M. E. Taylor Engineering

Phone 301-774-6246

Fax 301-774-6711

www.semsupplies.com

I'm sure he can give you what you need. Scintillators have been his
specialty for decades. Also, many of the other EM supply companies should
be able to provide what you're looking for.

Disclaimer: A number of years ago when my business was larger, I was a
distributor of Taylor supplies and have many happy customers.

Ken Converse
Owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca [mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca]
Sent: Wednesday, February 08, 2006 4:35 PM
To: kenconverse-at-qualityimages.biz

Hi everyone,

I will be experimenting with alternative scintillators for our Hitachi
S4800 STEM unit. As I was advised to no touch the scintillator in the
microscope, I hope someone will be able to offer measurements for the
original S4800 scintillator.

Thanks,
Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC
W6-017A ECERF Bldg, 9107-116 Street
Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632



==============================Original Headers==============================
5, 23 -- From Daniel.Salamon-at-nrc-cnrc.gc.ca Wed Feb 8 15:33:09 2006
5, 23 -- Received: from nrccenexf2.nrc.ca (nrccenexf2.nrc.ca
[132.246.15.83])
5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
k18LX8mG009977
5, 23 -- for {microscopy-at-microscopy.com} ; Wed, 8 Feb 2006 15:33:08
-0600
5, 23 -- Received: from nrccenexb2.nrc.ca ([132.246.15.98]) by
nrccenexf2.nrc.ca with Microsoft SMTPSVC(6.0.3790.1830);
5, 23 -- Wed, 8 Feb 2006 16:33:07 -0500
5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
5, 23 -- Content-class: urn:content-classes:message
5, 23 -- MIME-Version: 1.0
5, 23 -- Content-Type: text/plain;
5, 23 -- charset="us-ascii"
5, 23 -- Subject: S4800 STEM scintillator size
5, 23 -- Date: Wed, 8 Feb 2006 16:32:19 -0500
5, 23 -- Message-ID:
{923687253229634E96E808B8D3124C83557517-at-nrccenexb2.nrc.ca}
5, 23 -- X-MS-Has-Attach:
5, 23 -- X-MS-TNEF-Correlator:
5, 23 -- Thread-Topic: S4800 STEM scintillator size
5, 23 -- Thread-Index: AcYs9yOtLSo8zGW/Rh2wOcOIwp9aqA==
5, 23 -- From: "Salamon, Daniel" {Daniel.Salamon-at-nrc-cnrc.gc.ca}
5, 23 -- To: {microscopy-at-microscopy.com}
5, 23 -- X-OriginalArrivalTime: 08 Feb 2006 21:33:07.0447 (UTC)
FILETIME=[4054A070:01C62CF7]
5, 23 -- Content-Transfer-Encoding: 8bit
5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id k18LX8mG009977
==============================End of - Headers==============================





___________________________________________________________
$0 Web Hosting with up to 200MB web space, 1000 MB Transfer
10 Personalized POP and Web E-mail Accounts, and much more.
Signup at www.doteasy.com



==============================Original Headers==============================
26, 24 -- From kenconverse-at-qualityimages.biz Fri Feb 10 10:20:24 2006
26, 24 -- Received: from qualityimages.biz (dpmail16.doteasy.com [65.61.209.16])
26, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1AGKLGN000476
26, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 10 Feb 2006 10:20:22 -0600
26, 24 -- Received: from Ken [68.64.242.101] by qualityimages.biz with ESMTP
26, 24 -- (SMTPD32-8.05) id ADB831560154; Fri, 10 Feb 2006 08:22:16 -0800
26, 24 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
26, 24 -- To: {Daniel.Salamon-at-nrc-cnrc.gc.ca} ,
26, 24 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com}
26, 24 -- Subject: RE: [Microscopy] S4800 STEM scintillator size
26, 24 -- Date: Fri, 10 Feb 2006 07:20:44 -0500
26, 24 -- Message-ID: {003b01c62e3c$746b43a0$6501a8c0-at-Ken}
26, 24 -- MIME-Version: 1.0
26, 24 -- Content-Type: text/plain;
26, 24 -- charset="us-ascii"
26, 24 -- X-Priority: 3 (Normal)
26, 24 -- X-MSMail-Priority: Normal
26, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.6626
26, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
26, 24 -- Importance: Normal
26, 24 -- In-Reply-To: {200602082135.k18LZD0D015228-at-ns.microscopy.com}
26, 24 -- X-IMSTrailer: __IMail_7__
26, 24 -- Content-Transfer-Encoding: 8bit
26, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1AGKLGN000476
==============================End of - Headers==============================




From: RossLM-at-missouri.edu
Date: Fri, 10 Feb 2006 11:55:40 -0600
Subject: [Microscopy] 2 contract positions at Monsanto in St. Louis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I am posting the following ad for Dr. Jingyue Liu at Monsanto. Please
contact Dr. Liu for further information, email address at the bottom
of the request.

Lou Ross


Two Contract Researchers Are Needed in the Catalyst Characterization
Group of Monsanto Company

Job Location: Creve Coeur Campus, St. Louis, Missouri 63167

The following two openings (one year contract researchers) are
immediately available:

1) Research associate for TEM sample preparation. Required skills:
extensive experience and expertise in ultramicrotoming thin sections
of catalyst powders or other nanophase materials for transmission
electron microscopy observation. This job requires strong hands-on
skills and new method development for unique samples. Experience
with sample preparation equipments such as high vacuum carbon
coating, embedding, fixing and staining biological tissues, and
maintenance of sample preparation facility is highly desirable.
Experience in operating SEM instruments is a plus.

2) Research associate for catalyst preparation, treatment and
characterization. Required skills: extensive experience in preparing
model and practical catalysts or nanoparticles and TEM
characterization of such samples. Experience in catalyst treatment
and testing is a plus. Strong hands-on skills and demonstrated
capability of designing complex experiments are required for this job.

Both jobs require extensive hands-on experiences in research labs.
The following competencies are required: innovation in solving
challenging problems; good communication and interpersonal skills;
teamwork skills and results orientation.

Since Monsanto does not directly hire contract researchers, the
selected candidates will work for a contract agency. Interested
parties please send your resume and application letter to:
Jingyue.liu-at-monsanto.com.

--
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=2227
email: rosslm-at-missouri.edu
http://www.emc.missouri.edu

==============================Original Headers==============================
11, 17 -- From RossLM-at-missouri.edu Fri Feb 10 11:55:40 2006
11, 17 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49])
11, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1AHtd3Q012471
11, 17 -- for {microscopy-at-microscopy.com} ; Fri, 10 Feb 2006 11:55:39 -0600
11, 17 -- Received: from um-exproto9.um.umsystem.edu ([207.160.151.148]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830);
11, 17 -- Fri, 10 Feb 2006 11:55:39 -0600
11, 17 -- Received: from [128.206.78.231] ([128.206.78.231]) by um-exproto9.um.umsystem.edu over TLS secured channel with Microsoft SMTPSVC(6.0.3790.1830);
11, 17 -- Fri, 10 Feb 2006 11:55:39 -0600
11, 17 -- Mime-Version: 1.0
11, 17 -- X-Sender: RossLM-at-pop.missouri.edu
11, 17 -- Message-Id: {p05200f30c0128247bb75-at-[128.206.78.231]}
11, 17 -- Date: Fri, 10 Feb 2006 11:55:37 -0600
11, 17 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com}
11, 17 -- From: Lou Ross {RossLM-at-missouri.edu}
11, 17 -- Subject: 2 contract positions at Monsanto in St. Louis
11, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
11, 17 -- X-OriginalArrivalTime: 10 Feb 2006 17:55:39.0395 (UTC) FILETIME=[33E96530:01C62E6B]
==============================End of - Headers==============================




From: ldmm-at-risc4.numis.northwestern.edu
Date: Fri, 10 Feb 2006 12:25:19 -0600
Subject: [Microscopy] Texts that combine TEM + scanning probe techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I am comtemplating broadening a mainly TEM undergrad course (with a lab)
to include some scanning probe techniques. Does anyone know of reasonable
texts which have some coverage?

-----------------------------------------------
Laurence Marks
Department of Materials Science and Engineering
MSE Rm 2036 Cook Hall
2220 N Campus Drive
Northwestern University
Evanston, IL 60201, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
email: L - marks -at- northwestern . edu
http://www.numis.northwestern.edu
-----------------------------------------------



==============================Original Headers==============================
4, 16 -- From ldmm-at-risc4.numis.northwestern.edu Fri Feb 10 12:25:19 2006
4, 16 -- Received: from risc4.numis.northwestern.edu (risc4.numis.northwestern.edu [129.105.122.70])
4, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1AIPJKL022849
4, 16 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Feb 2006 12:25:19 -0600
4, 16 -- Received: from localhost (ldmm-at-localhost)
4, 16 -- by risc4.numis.northwestern.edu (8.9.3 (PHNE_28760_binary)/8.9.3) with ESMTP id MAA24082
4, 16 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Feb 2006 12:25:14 -0600 (CST)
4, 16 -- Date: Fri, 10 Feb 2006 12:25:14 -0600 (CST)
4, 16 -- From: LDM Microscopy {ldmm-at-risc4.numis.northwestern.edu}
4, 16 -- To: MSA listserver {Microscopy-at-microscopy.com}
4, 16 -- Subject: Texts that combine TEM + scanning probe techniques
4, 16 -- In-Reply-To: {000001c494e3$2098d330$b99bd280-at-paklabpgrover}
4, 16 -- Message-ID: {Pine.GHP.4.63.0602101223340.24076-at-risc4.numis.northwestern.edu}
4, 16 -- References: {000001c494e3$2098d330$b99bd280-at-paklabpgrover}
4, 16 -- MIME-Version: 1.0
4, 16 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
==============================End of - Headers==============================




From: anna8261-at-yahoo.com
Date: Sat, 11 Feb 2006 17:21:03 -0600
Subject: [Microscopy] AskAMicroscopist: heterostructure InP/InGaAsP

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (anna8261-at-yahoo.com) from
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Friday, February 10, 2006 at 02:52:59
---------------------------------------------------------------------------

Email: anna8261-at-yahoo.com
Name: anna naghshegar

Organization: pooysh

Education: Graduate College

Location: tehran, iran

Question: what is stain solution for double heterostructure InP/InGaAsP?

---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Sat Feb 11 17:21:03 2006
7, 12 -- Received: from [203.98.91.39] (msdvpn27.msd.anl.gov [130.202.238.91])
7, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1BNKxBD017394
7, 12 -- for {microscopy-at-microscopy.com} ; Sat, 11 Feb 2006 17:21:01 -0600
7, 12 -- Mime-Version: 1.0
7, 12 -- X-Sender: (Unverified)
7, 12 -- Message-Id: {p06020404c01421c5adc8-at-[203.98.91.39]}
7, 12 -- Date: Sun, 12 Feb 2006 10:20:58 +1100
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: anna8261-at-yahoo.com (by way of Ask-A-Microscopist)
7, 12 -- Subject: AskAMicroscopist: heterostructure InP/InGaAsP
7, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: cbalane-at-wesleyan.edu
Date: Sun, 12 Feb 2006 01:38:59 -0600
Subject: [Microscopy] colloidal gold structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello listservers,
I realize that this might be a silly question but I was wondering if there
was anyone here who knows what the structure of colloidal gold is.

Thanks,
Carlo


--
Carlo Franco Bolivar Balane

Box 4058, 222 Church Street, or Wolfe Laboratory
Wesleyan University Station Rm. 157, HA Laboratories
Middletown, CT, 06459-4058 Wesleyan University



==============================Original Headers==============================
7, 32 -- From cbalane-at-wesleyan.edu Sun Feb 12 01:38:59 2006
7, 32 -- Received: from post1.wesleyan.edu (post1.wesleyan.edu [129.133.6.131])
7, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1C7cxSn031796
7, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 01:38:59 -0600
7, 32 -- Received: from pony1.wesleyan.edu (pony1.wesleyan.edu [129.133.6.192])
7, 32 -- by post1.wesleyan.edu (8.12.11/8.12.11) with ESMTP id k1C7dAPY015816
7, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 02:39:10 -0500
7, 32 -- Received: from pony1.wesleyan.edu (pony1.wesleyan.edu [127.0.0.1])
7, 32 -- by pony1.wesleyan.edu (8.12.11/8.12.11) with ESMTP id k1C7d045011036
7, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 02:39:00 -0500
7, 32 -- Received: (from apache-at-localhost)
7, 32 -- by pony1.wesleyan.edu (8.12.11/8.12.11/Submit) id k1C7d0xP011034;
7, 32 -- Sun, 12 Feb 2006 02:39:00 -0500
7, 32 -- Received: from 129.133.127.145
7, 32 -- (SquirrelMail authenticated user cbalane);
7, 32 -- by webmail.wesleyan.edu with HTTP;
7, 32 -- Sun, 12 Feb 2006 02:39:00 -0500 (EST)
7, 32 -- Message-ID: {2201.129.133.127.145.1139729940.squirrel-at-129.133.127.145}
7, 32 -- Date: Sun, 12 Feb 2006 02:39:00 -0500 (EST)
7, 32 -- Subject: colloidal gold structure
7, 32 -- From: "Carlo Franco Balane-Bolivar" {cbalane-at-wesleyan.edu}
7, 32 -- To: Microscopy-at-microscopy.com
7, 32 -- User-Agent: SquirrelMail/1.4.3a-0.e3.1
7, 32 -- X-Mailer: SquirrelMail/1.4.3a-0.e3.1
7, 32 -- MIME-Version: 1.0
7, 32 -- Content-Type: text/plain;charset=iso-8859-1
7, 32 -- Content-Transfer-Encoding: 8bit
7, 32 -- X-Priority: 3 (Normal)
7, 32 -- Importance: Normal
7, 32 -- X-Wesleyan-MailScanner-Information: Please contact the ISP for more information
7, 32 -- X-Wesleyan-MailScanner: Found to be clean
7, 32 -- X-MailScanner-From: cbalane-at-wesleyan.edu
==============================End of - Headers==============================




From: neil-at-young8696.freeserve.co.uk
Date: Sun, 12 Feb 2006 06:20:41 -0600
Subject: [Microscopy] RE: colloidal gold structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

In general multiply-twinned fcc domains I would have thought. Although depending on particle size, very small colloids can have structure forbidden by conventional bulk crystallography such as a 5-fold icosahedral or decahedral arrangements, plenty of literature around, both experimental and simulations. I seem to remember passivation of the gold 'cluster' can force the resulting colloid into certain structures. Generally Au over 3-4nm in diameter is mt-fcc though.

Neil Young
Cluster Physics / Electron Microscopy
Nanoscale Physics Research Laboratory
School of Physics and Astronomy
University of Birmingham
UK



} Message Received: Feb 12 2006, 07:42 AM
} From: cbalane-at-wesleyan.edu
} To: neil-at-young8696.freeserve.co.uk
} Cc:
} Subject: [Microscopy] colloidal gold structure
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello listservers,
} I realize that this might be a silly question but I was wondering if there
} was anyone here who knows what the structure of colloidal gold is.
}
} Thanks,
} Carlo
}
}
} --
} Carlo Franco Bolivar Balane
}
} Box 4058, 222 Church Street, or Wolfe Laboratory
} Wesleyan University Station Rm. 157, HA Laboratories
} Middletown, CT, 06459-4058 Wesleyan University
}
}
}
} ==============================Original Headers==============================
} 7, 32 -- From cbalane-at-wesleyan.edu Sun Feb 12 01:38:59 2006
} 7, 32 -- Received: from post1.wesleyan.edu (post1.wesleyan.edu [129.133.6.131])
} 7, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1C7cxSn031796
} 7, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 01:38:59 -0600
} 7, 32 -- Received: from pony1.wesleyan.edu (pony1.wesleyan.edu [129.133.6.192])
} 7, 32 -- by post1.wesleyan.edu (8.12.11/8.12.11) with ESMTP id k1C7dAPY015816
} 7, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 02:39:10 -0500
} 7, 32 -- Received: from pony1.wesleyan.edu (pony1.wesleyan.edu [127.0.0.1])
} 7, 32 -- by pony1.wesleyan.edu (8.12.11/8.12.11) with ESMTP id k1C7d045011036
} 7, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 02:39:00 -0500
} 7, 32 -- Received: (from apache-at-localhost)
} 7, 32 -- by pony1.wesleyan.edu (8.12.11/8.12.11/Submit) id k1C7d0xP011034;
} 7, 32 -- Sun, 12 Feb 2006 02:39:00 -0500
} 7, 32 -- Received: from 129.133.127.145
} 7, 32 -- (SquirrelMail authenticated user cbalane);
} 7, 32 -- by webmail.wesleyan.edu with HTTP;
} 7, 32 -- Sun, 12 Feb 2006 02:39:00 -0500 (EST)
} 7, 32 -- Message-ID: {2201.129.133.127.145.1139729940.squirrel-at-129.133.127.145}
} 7, 32 -- Date: Sun, 12 Feb 2006 02:39:00 -0500 (EST)
} 7, 32 -- Subject: colloidal gold structure
} 7, 32 -- From: "Carlo Franco Balane-Bolivar" {cbalane-at-wesleyan.edu}
} 7, 32 -- To: Microscopy-at-microscopy.com
} 7, 32 -- User-Agent: SquirrelMail/1.4.3a-0.e3.1
} 7, 32 -- X-Mailer: SquirrelMail/1.4.3a-0.e3.1
} 7, 32 -- MIME-Version: 1.0
} 7, 32 -- Content-Type: text/plain;charset=iso-8859-1
} 7, 32 -- Content-Transfer-Encoding: 8bit
} 7, 32 -- X-Priority: 3 (Normal)
} 7, 32 -- Importance: Normal
} 7, 32 -- X-Wesleyan-MailScanner-Information: Please contact the ISP for more information
} 7, 32 -- X-Wesleyan-MailScanner: Found to be clean
} 7, 32 -- X-MailScanner-From: cbalane-at-wesleyan.edu
} ==============================End of - Headers==============================
}
}


==============================Original Headers==============================
6, 25 -- From neil-at-young8696.freeserve.co.uk Sun Feb 12 06:20:41 2006
6, 25 -- Received: from smtp2.freeserve.com (smtp2.wanadoo.co.uk [193.252.22.157])
6, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1CCKd7F018464
6, 25 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 06:20:40 -0600
6, 25 -- Received: from me-wanadoo.net (localhost [127.0.0.1])
6, 25 -- by mwinf3106.me.freeserve.com (SMTP Server) with ESMTP id 266D41C00085
6, 25 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 13:20:37 +0100 (CET)
6, 25 -- Received: from wwinf3103 (wwinf3103 [172.22.158.30])
6, 25 -- by mwinf3106.me.freeserve.com (SMTP Server) with ESMTP id 205A51C00083
6, 25 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 13:20:37 +0100 (CET)
6, 25 -- X-ME-UUID: 20060212122037132.205A51C00083-at-mwinf3106.me.freeserve.com
6, 25 -- Message-ID: {30650493.1139746837118.JavaMail.www-at-wwinf3103}
6, 25 -- From: "Neil P. Young" {neil-at-young8696.freeserve.co.uk}
6, 25 -- Reply-To: neil-at-young8696.freeserve.co.uk
6, 25 -- To: Microscopy-at-microscopy.com
6, 25 -- Subject: RE: [Microscopy] colloidal gold structure
6, 25 -- Mime-Version: 1.0
6, 25 -- Content-Type: text/plain; charset=UTF-8
6, 25 -- Content-Transfer-Encoding: 7bit
6, 25 -- X-Originating-IP: [195.92.168.168]
6, 25 -- X-Wum-Nature: EMAIL-NATURE
6, 25 -- X-WUM-FROM: |~|
6, 25 -- X-WUM-TO: |~|
6, 25 -- X-WUM-REPLYTO: |~|
6, 25 -- Date: Sun, 12 Feb 2006 13:20:37 +0100 (CET)
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Sun, 12 Feb 2006 14:39:26 -0600
Subject: [Microscopy] SEM x-ray peak ratios

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Karl,
I hadn't seen any answer to your question so I'll give it a shot. For those
of us who grew up with Be windows, K bigger than L seems normal. However,
the norm for light element detectors is the opposite, at least if you
normally operate at 20kV or so. I'm wondering if you've had a long-standing
contamination problem with your light element window. Now that it's clean,
you may be seeing the correct ratio and may find that your light element
detection is also better.

The other thing to be certain of is that you are operating at the same kV as
you were earlier. Lower kV will favor lower energy peaks and higher kV will
favor higher energy peaks. It can be quite dramatic. Put a little graphite
on a piece of Cu tape and collect a spectrum from an area containing both.
Maintain a constant dead-time while collecting a spectrum at 30 kV and
another at about 2 kV. You'd never know it's the same sample.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: hagglundk1-at-nku.edu [mailto:hagglundk1-at-nku.edu]
Sent: Tuesday, January 24, 2006 10:33 AM
To: kenconverse-at-qualityimages.biz

I have a colleague who recently experienced some down time on his SEM.
The system went through repeated cycles of pump down, and venting and
was eventually left powered down while waiting on a computer
replacement.

After getting everything operating again, an oil film was found that had
built up on the x-ray detector thin window. This was initially causing
an unacceptable amount of background in the 0-3kV range of the x-ray
signal as well as an overall low count rate. The collimator was removed
and cleaned, which corrected the noise and count rate. They are in the
process of replacing the roughing lines and cleaning the chamber to
prevent further contamination. Now, when calibrating with a copper
standard, the ratio of the L line signal versus the K lines is
dramatically favoring the L. This was not the case before.

Any ideas why this might be happening and how to correct it?

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu


==============================Original Headers==============================
5, 23 -- From hagglundk1-at-nku.edu Tue Jan 24 09:02:19 2006
5, 23 -- Received: from mailfe2.nku.edu (mailfe2.nku.edu [192.122.237.68])
5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
k0OF2JlW001861
5, 23 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2006 09:02:19
-0600
5, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by
mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211);
5, 23 -- Tue, 24 Jan 2006 10:02:19 -0500
5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0
5, 23 -- Content-class: urn:content-classes:message
5, 23 -- MIME-Version: 1.0
5, 23 -- Content-Type: text/plain;
5, 23 -- charset="us-ascii"
5, 23 -- Subject: SEM x-ray peak ratios
5, 23 -- Date: Tue, 24 Jan 2006 10:02:18 -0500
5, 23 -- Message-ID:
{F01E4499C4EC5842A8AB7198141AC72F3585FA-at-mailfac1.hh.nku.edu}
5, 23 -- X-MS-Has-Attach:
5, 23 -- X-MS-TNEF-Correlator:
5, 23 -- Thread-Topic: SEM x-ray peak ratios
5, 23 -- Thread-Index: AcYg9yuMm942rGb1QfKCevfjxyMD/g==
5, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu}
5, 23 -- To: {microscopy-at-microscopy.com}
5, 23 -- X-OriginalArrivalTime: 24 Jan 2006 15:02:19.0222 (UTC)
FILETIME=[2BE72F60:01C620F7]
5, 23 -- Content-Transfer-Encoding: 8bit
5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id k0OF2JlW001861
==============================End of - Headers==============================





___________________________________________________________
$0 Web Hosting with up to 200MB web space, 1000 MB Transfer
10 Personalized POP and Web E-mail Accounts, and much more.
Signup at www.doteasy.com



==============================Original Headers==============================
22, 23 -- From kenconverse-at-qualityimages.biz Sun Feb 12 14:39:26 2006
22, 23 -- Received: from qualityimages.biz (dpmail16.doteasy.com [65.61.209.16])
22, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1CKdPhn012116
22, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sun, 12 Feb 2006 14:39:26 -0600
22, 23 -- Received: from Ken [68.64.242.101] by qualityimages.biz with ESMTP
22, 23 -- (SMTPD32-8.05) id AD6DA2CE00B8; Sun, 12 Feb 2006 12:41:17 -0800
22, 23 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
22, 23 -- To: {hagglundk1-at-nku.edu} , "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com}
22, 23 -- Subject: RE: [Microscopy] SEM x-ray peak ratios
22, 23 -- Date: Sun, 12 Feb 2006 15:38:54 -0500
22, 23 -- Message-ID: {001701c63014$5fed1550$6501a8c0-at-Ken}
22, 23 -- MIME-Version: 1.0
22, 23 -- Content-Type: text/plain;
22, 23 -- charset="us-ascii"
22, 23 -- X-Priority: 3 (Normal)
22, 23 -- X-MSMail-Priority: Normal
22, 23 -- X-Mailer: Microsoft Outlook, Build 10.0.6626
22, 23 -- Importance: Normal
22, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
22, 23 -- In-Reply-To: {200601241533.k0OFX9f9007423-at-ns.microscopy.com}
22, 23 -- X-IMSTrailer: __IMail_7__
22, 23 -- Content-Transfer-Encoding: 8bit
22, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1CKdPhn012116
==============================End of - Headers==============================




From: George.Theodossiou-at-amcor.com.au
Date: Sun, 12 Feb 2006 18:58:09 -0600
Subject: [Microscopy] Nikon Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

A colleague has a Nikon SMZ-2T Microscope that he is trying to rescue. He
is after a copy of the manual. If anyone can help pls contact him at
hbeh-at-hotmail.com or via myself.

Thank you for your help

Regards
George



************************************************************************
CAUTION - This message may contain privileged and confidential
information intended only for the use of the addressee named above.
If you are not the intended recipient of this message you are hereby
notified that any use, dissemination, distribution or reproduction of
this message is prohibited. If you have received this message in error
please notify AMCOR immediately.
Any views expressed in this message are those of the individual sender
and may not necessarily reflect the views of AMCOR.
************************************************************************


==============================Original Headers==============================
8, 27 -- From George.Theodossiou-at-amcor.com.au Sun Feb 12 18:58:09 2006
8, 27 -- Received: from aiti251.amcor.com.au (mail.amcor.com.au [202.14.180.248] (may be forged))
8, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1D0w78V024534
8, 27 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 18:58:08 -0600
8, 27 -- Received: from aadcex0002.amcor.net (unverified) by aiti251.amcor.com.au
8, 27 -- (Content Technologies SMTPRS 4.3.19) with ESMTP id
8, 27 -- {T766fb3d9f0a0de98c8538-at-aiti251.amcor.com.au} for
8, 27 -- {Microscopy-at-microscopy.com} ; Mon, 13 Feb 2006 12:03:18 +1100
8, 27 -- Received: from aadcex0004.amcor.net ([160.222.80.235]) by
8, 27 -- aadcex0002.amcor.net with Microsoft SMTPSVC (6.0.3790.211); Mon, 13 Feb
8, 27 -- 2006 11:58:05 +1100
8, 27 -- Received: from 160.222.214.35 ([160.222.214.35]) by aadcex0004.amcor.net
8, 27 -- ([160.222.80.235]) with Microsoft Exchange Server HTTP-DAV; Mon, 13 Feb
8, 27 -- 2006 00:58:05 +0000
8, 27 -- User-Agent: Microsoft-Entourage/11.2.1.051004
8, 27 -- Date: Mon, 13 Feb 2006 11:57:31 +1100
8, 27 -- Subject: Nikon Microscope
8, 27 -- From: "George.Theodossiou" {George.Theodossiou-at-amcor.com.au}
8, 27 -- To: {Microscopy-at-microscopy.com}
8, 27 -- Message-ID: {C01624AB.8E6%George.Theodossiou-at-Amcor.com.au}
8, 27 -- Thread-Topic: Nikon Microscope
8, 27 -- Thread-Index: AcYwOHehtgRappwrEdqDLgANkzYUMg==
8, 27 -- Mime-version: 1.0
8, 27 -- Content-type: text/plain; charset="US-ASCII"
8, 27 -- Content-transfer-encoding: 7bit
8, 27 -- X-OriginalArrivalTime: 13 Feb 2006 00:58:06.0019 (UTC)
8, 27 -- FILETIME=[8C80C930:01C63038]
==============================End of - Headers==============================




From: gerd.leitinger-at-meduni-graz.at
Date: Mon, 13 Feb 2006 04:22:44 -0600
Subject: [Microscopy] EM: precautions when en bloc staining summary

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Thank you everybody who replied to my question regarding safety
measures when working with uranyl acetate stained blocks.

To sum up the answers: The binding capacity of tissue to uranyl acetate is
apparently low and therefore very little (if any) radioactivity can escape
the blocks. However, when trimming I have been advised to wear gloves
and
a mask to prevent myself from inhaling chips from the specimen, and it
seems necessary that we collect the chips and dispose of them as
radioactive waste.

thank you

Gerd



Dr. Gerd Leitinger

Institut für Zellbiologie, Histologie und Embryologie
Medizinische Universität Graz
Harrachgasse 21
A-8010 Graz
Austria

Tel. ++43 316 380 4237
Fax. ++43 316 380 9625
Mailto: Gerd.Leitinger-at-meduni-graz.at



==============================Original Headers==============================
10, 19 -- From gerd.leitinger-at-meduni-graz.at Mon Feb 13 04:22:44 2006
10, 19 -- Received: from herakles.kfunigraz.ac.at (HERAKLES.kfunigraz.ac.at [143.50.13.36])
10, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1DAMhWM008929
10, 19 -- for {microscopy-at-microscopy.com} ; Mon, 13 Feb 2006 04:22:44 -0600
10, 19 -- Received: from [10.200.112.73] ([193.170.104.185]) by herakles.kfunigraz.ac.at with Microsoft SMTPSVC(5.0.2195.5329);
10, 19 -- Mon, 13 Feb 2006 11:22:20 +0100
10, 19 -- From: "Gerd Leitinger" {gerd.leitinger-at-meduni-graz.at}
10, 19 -- To: microscopy-at-microscopy.com
10, 19 -- Date: Mon, 13 Feb 2006 11:17:48 +0100
10, 19 -- MIME-Version: 1.0
10, 19 -- Subject: EM: precautions when en bloc staining summary
10, 19 -- Message-ID: {43F06ADD.13462.738363-at-gerd.leitinger.meduni-graz.at}
10, 19 -- Priority: normal
10, 19 -- X-mailer: Pegasus Mail for Windows (4.31, DE v4.31 R1)
10, 19 -- Content-type: text/plain; charset=ISO-8859-1
10, 19 -- Content-description: Mail message body
10, 19 -- X-OriginalArrivalTime: 13 Feb 2006 10:22:20.0817 (UTC) FILETIME=[5F890010:01C63087]
10, 19 -- Content-Transfer-Encoding: 8bit
10, 19 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id k1DAMhWM008929
==============================End of - Headers==============================




From: aarti_harle-at-yahoo.co.in
Date: Mon, 13 Feb 2006 04:53:08 -0600
Subject: [Microscopy] confocal microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear All

We are trying to viusalize infected erythrocytes
(malarial parasite) on confocal microscope by indirect
fluorescence without any fixation and washing is
carried out with PBS

The immages are not satisfactory because
fading/bleaching is fast though we are using % DABCO
in 50% glycerol and secondly lot of background noise.

Most of the literature shows the fixation with
ethanol/methenol but I would like to carry out the
fixation with paraformaldehyde for the obvious reason
and washing with MSM-PIPES with tris.

any suggestion????
or any body can describle the sample preparation for
infected erythrocytes using paraformaldehy as a
fixative and MSM PIPES as a washing buffer...

Does non fixation of specimen play any role in fast
bleaching???

Regards
Shrunali
Scientist
IMTECH, India



__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

==============================Original Headers==============================
10, 18 -- From aarti_harle-at-yahoo.co.in Mon Feb 13 04:53:08 2006
10, 18 -- Received: from web8308.mail.in.yahoo.com (web8308.mail.in.yahoo.com [202.43.219.220])
10, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1DAr74m018713
10, 18 -- for {Microscopy-at-Microscopy.com} ; Mon, 13 Feb 2006 04:53:07 -0600
10, 18 -- Received: (qmail 90502 invoked by uid 60001); 13 Feb 2006 10:53:05 -0000
10, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
10, 18 -- s=s1024; d=yahoo.co.in;
10, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
10, 18 -- b=XV1GTCKSYckbhHBodcI+9aRMHIyL3k7aSk/fx0siVLqY2GubzN4t3ETtsQ5SvUNHdvo0HZCbwY0LNwqIEVo5wG0A2w5hFGhRK2sdU4Sw5T+v7Wv8JD5Q0rRO14w39oy6TAwfWGnMr0IXewk2yCdWRCswRSZSwMNHWt722M1sryM= ;
10, 18 -- Message-ID: {20060213105305.90500.qmail-at-web8308.mail.in.yahoo.com}
10, 18 -- Received: from [203.197.210.215] by web8308.mail.in.yahoo.com via HTTP; Mon, 13 Feb 2006 02:53:05 PST
10, 18 -- Date: Mon, 13 Feb 2006 02:53:05 -0800 (PST)
10, 18 -- From: Aarti Harle {aarti_harle-at-yahoo.co.in}
10, 18 -- Subject: confocal microscopy
10, 18 -- To: Microscopy-at-Microscopy.com
10, 18 -- MIME-Version: 1.0
10, 18 -- Content-Type: text/plain; charset=iso-8859-1
10, 18 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: phillipst-at-missouri.edu
Date: Mon, 13 Feb 2006 08:58:53 -0600
Subject: [Microscopy] Re: confocal microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

You don't specify which fluorochrome you are using. If you are using FITC
or Rhodamine, that is one of the reasons you are getting rapid fading. The
Alexa488 and Alexa568 fluorochromes from Molecular Probes (Invitrogen) are
far superior in this regard - especially for confocal. Molecular Probes
also has a new anti-fade mounting medium called Prolong Gold but I don't
have enough experience at the moment to discuss its efficacy. Fixation
will not have any effect on bleaching but will contribute to background
noise (especially glutaraldehyde). Generally 2% PF is okay in regards to
background fluorescence and sometimes it is safe to add 0.1 - 02%
glutaraldehyde. Aldehyde fixatives may, however, interfere with your
antibody recognizing its epitope. good luck.

rote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



==============================Original Headers==============================
9, 21 -- From PhillipsT-at-missouri.edu Mon Feb 13 08:58:53 2006
9, 21 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49])
9, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1DEwqQ7031787
9, 21 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 13 Feb 2006 08:58:52 -0600
9, 21 -- Received: from um-exproto9.um.umsystem.edu ([207.160.151.148]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830);
9, 21 -- Mon, 13 Feb 2006 08:58:52 -0600
9, 21 -- Received: from phillips-dell.missouri.edu ([128.206.81.132]) by um-exproto9.um.umsystem.edu over TLS secured channel with Microsoft SMTPSVC(6.0.3790.1830);
9, 21 -- Mon, 13 Feb 2006 08:58:51 -0600
9, 21 -- Message-Id: {6.0.0.22.2.20060213085354.057faf00-at-pop.missouri.edu}
9, 21 -- X-Sender: phillipst-at-pop.missouri.edu
9, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 6.0.0.22
9, 21 -- Date: Mon, 13 Feb 2006 08:58:49 -0600
9, 21 -- To: aarti_harle-at-yahoo.co.in
9, 21 -- From: Tom Phillips {phillipst-at-missouri.edu}
9, 21 -- Subject: Re: [Microscopy] confocal microscopy
9, 21 -- Cc: Microscopy-at-msa.microscopy.com
9, 21 -- In-Reply-To: {200602131054.k1DAseRm020277-at-ns.microscopy.com}
9, 21 -- References: {200602131054.k1DAseRm020277-at-ns.microscopy.com}
9, 21 -- Mime-Version: 1.0
9, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
9, 21 -- X-OriginalArrivalTime: 13 Feb 2006 14:58:52.0132 (UTC) FILETIME=[00BAC240:01C630AE]
==============================End of - Headers==============================




From: dljones-at-bestweb.net
Date: Mon, 13 Feb 2006 09:22:45 -0600
Subject: [Microscopy] Zeiss 940A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I'm working on getting a Zeiss 940A up and running for my local high school. We
are working on a shoe-string budget as one may expect from a small public high
school.

I am looking for technical documentation for this instrument so that I can try
and get it functioning. If anyone has schematics or any other technical
documentation for instrument this I would greatly appreciate getting copies in
some way.

If anyone has a similar instrument that would be able to donate it to us for a
spare parts machine, we would come to pick it up. We are located in the Hudson
Valley.

I am willing to pay for copies if need be, as well as transportation costs.

Thank you in advance,

dj


==============================Original Headers==============================
7, 22 -- From dljones-at-bestweb.net Mon Feb 13 09:22:44 2006
7, 22 -- Received: from smtp2.bestweb.net (smtp2.bestweb.net [209.94.103.44])
7, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1DFMigH008985
7, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Feb 2006 09:22:44 -0600
7, 22 -- Received: from smtp2.bestweb.net (localhost [127.0.0.1])
7, 22 -- by smtp2.bestweb.net (Postfix) with ESMTP id 0659D1CD06
7, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Feb 2006 10:22:42 -0500 (EST)
7, 22 -- Received: from dlj (pool-71-249-9-96.nycmny.east.verizon.net [71.249.9.96])
7, 22 -- by smtp2.bestweb.net (Postfix) with ESMTP id 9164D1CCCB
7, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Feb 2006 10:22:41 -0500 (EST)
7, 22 -- Date: Mon, 13 Feb 2006 10:31:56 -0500 (Eastern Standard Time)
7, 22 -- From: "David L. Jones" {dljones-at-bestweb.net}
7, 22 -- To: Microscopy-at-microscopy.com
7, 22 -- Subject: Zeiss 940A
7, 22 -- Message-ID: {Pine.WNT.4.62.0602131022110.1512-at-dlj}
7, 22 -- X-X-Sender: dljones-at-pop-croton.bestweb.net
7, 22 -- MIME-Version: 1.0
7, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
7, 22 -- X-Spam-Checker-Version: SpamAssassin 3.0.2 (2004-11-16) on smtp2-1.bestweb.net
7, 22 -- X-Spam-Level:
7, 22 -- X-Spam-Status: No, score=-2.8 required=5.0 tests=ALL_TRUSTED autolearn=failed
7, 22 -- version=3.0.2
==============================End of - Headers==============================




From: roncastellano-at-adelphia.net
Date: Mon, 13 Feb 2006 14:43:56 -0600
Subject: [Microscopy] AskAMicroscopist: Assistance to setup a JOEL JSM-35C SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question was submitted to Ask-A-Microscopist by
(roncastellano-at-adelphia.net)
from
http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
on Monday, February 13, 2006 at 13:21:29
Remember to consider the Grade/Age of the student when considering the Question
---------------------------------------------------------------------------
Please reply to both roncastellano-at-adelphia.net as well as to the
Microscopy Listserver
---------------------------------------------------------------------------

Email: roncastellano-at-adelphia.net
Name: Ron Castellano

Organization: Wolfram Analytical Labs

Education: Undergraduate College

Location: 924 Fords Corner Road, Nanty Glo, PA 15943

Title: Assistance to setup a JOEL JSM-35C SEM

Question: I'm a retired assay technician(precious metal analysis)
I have purchased an old SEM for private use in a small lab I am building here.
I need someone to set up and test unit at my site. The unit is said
to be operational when de-installed, it appears to be in good shape.
Are there any technicians, perhaps a graduate student, who can assist
me. Of course I am willing to pay any reasonable fee for this
service. Ron Castellano

---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Mon Feb 13 14:43:55 2006
8, 12 -- Received: from [203.33.121.122] (msdvpn21.msd.anl.gov [130.202.238.85])
8, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1DKhmJ4023798
8, 12 -- for {microscopy-at-microscopy.com} ; Mon, 13 Feb 2006 14:43:50 -0600
8, 12 -- Mime-Version: 1.0
8, 12 -- X-Sender: (Unverified)
8, 12 -- Message-Id: {p06020409c0169fdebb84-at-[203.33.121.122]}
8, 12 -- Date: Tue, 14 Feb 2006 07:43:44 +1100
8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: roncastellano-at-adelphia.net (by way of Ask-A-Microscopist)
8, 12 -- Subject: AskAMicroscopist: Assistance to setup a JOEL JSM-35C SEM
8, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: ken.blight-at-cancer.org.uk
Date: Wed, 15 Feb 2006 09:24:56 -0600
Subject: [Microscopy] SEM of Protozoa

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

It is likely that I will need to view protozoa, in the SEM, in the near
future.Is there a special processing protocol or any problems to look out
for.


==============================Original Headers==============================
2, 19 -- From ken.blight-at-cancer.org.uk Wed Feb 15 09:24:56 2006
2, 19 -- Received: from crukexch1.crwin.crnet.org (mailexch.cancer.org.uk [143.65.1.2])
2, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1FFOtYt007581
2, 19 -- for {microscopy-at-microscopy.com} ; Wed, 15 Feb 2006 09:24:55 -0600
2, 19 -- Received: from crukexch3.crwin.crnet.org ([143.65.1.113]) by crukexch1.crwin.crnet.org with Microsoft SMTPSVC(6.0.3790.1830);
2, 19 -- Wed, 15 Feb 2006 15:24:54 +0000
2, 19 -- Received: from 143.65.59.12 ([143.65.59.12]) by crukexch3.crwin.crnet.org ([143.65.1.113]) with Microsoft Exchange Server HTTP-DAV ;
2, 19 -- Wed, 15 Feb 2006 15:24:53 +0000
2, 19 -- User-Agent: Microsoft-Entourage/11.0.0.040405
2, 19 -- Date: Wed, 15 Feb 2006 15:24:53 +0000
2, 19 -- Subject: SEM of Protozoa
2, 19 -- From: Ken Blight {ken.blight-at-cancer.org.uk}
2, 19 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
2, 19 -- Message-ID: {C018F845.76A%ken.blight-at-cancer.org.uk}
2, 19 -- Mime-version: 1.0
2, 19 -- Content-type: text/plain;
2, 19 -- charset="US-ASCII"
2, 19 -- Content-transfer-encoding: 7bit
2, 19 -- X-OriginalArrivalTime: 15 Feb 2006 15:24:54.0326 (UTC) FILETIME=[F8B22560:01C63243]
==============================End of - Headers==============================




From: eoptics-at-mcmaster.ca
Date: Wed, 15 Feb 2006 09:52:58 -0600
Subject: [Microscopy] TEM, Gatan 673 Wide Angle Camera, Looking for Spare Parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Good Day Readers:

I am in need of a working video card for a Gatan 673 Wide TV Camera,
vintage 1987.
This camera system is used on a Philips CM12 for teaching purposes.
The video card model is Schlumberger/Fairchild FAB-2-1-28 rev. F.

I will consider buying the controller if you do not wish to sell the card
separately.

If you wish you can contact me offline.

Thanks in advance,

Fred Pearson

*******************************************
Fred Pearson
McMaster University
Brockhouse Institute for Materials Research
ABB-B145
1280 Main Street West
Hamilton ON. Canada
L8S 4M1

email:eoptics-at-mcmaster.ca
phone: (905) 525-9140 ext.24609
fax: (905) 521-2773
*******************************************

==============================Original Headers==============================
9, 15 -- From eoptics-at-mcmaster.ca Wed Feb 15 09:52:58 2006
9, 15 -- Received: from cgpsrv2.cis.mcmaster.ca (univmail.CIS.mcmaster.ca [130.113.64.46])
9, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1FFqwVp017516
9, 15 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Feb 2006 09:52:58 -0600
9, 15 -- Received: from pc-pearson-f2.imr.McMaster.CA ([130.113.54.155] verified)
9, 15 -- by cgpsrv2.cis.mcmaster.ca (CommuniGate Pro SMTP 4.1.8)
9, 15 -- with ESMTP-TLS id 117876491 for Microscopy-at-microscopy.com; Wed, 15 Feb 2006 10:52:58 -0500
9, 15 -- Date: Wed, 15 Feb 2006 10:55:38 -0500 (Eastern Standard Time)
9, 15 -- From: Fred Pearson {eoptics-at-mcmaster.ca}
9, 15 -- To: Microscopy-at-microscopy.com
9, 15 -- Subject: TEM, Gatan 673 Wide Angle Camera, Looking for Spare Parts
9, 15 -- Message-ID: {Pine.WNT.4.58.0602151051420.484-at-tiberius}
9, 15 -- X-X-Sender: eoptics-at-univmail.cis.mcmaster.ca
9, 15 -- MIME-Version: 1.0
9, 15 -- Content-Type: TEXT/PLAIN; charset=US-ASCII
==============================End of - Headers==============================




From: wim5-at-lehigh.edu
Date: Wed, 15 Feb 2006 12:43:10 -0600
Subject: [Microscopy] JEOL JSM-6300f FEGSEM for sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Lehigh University, Center for Advanced Materials and Nanotechnology,
Bethlehem, PA is offering for sale a JEOL-6300F FEGSEM which will be
decommissioned in mid-March 2006. The Microscope includes an Oxford/ISIS
thin window EDS system, JEOL dry airlock and ARC64 digital imaging
system, solid state backscatter detector, IR chamberview camera. Asking
price: $60K Interested parties can contact Dr. Chris Kiely at
chk5-at-lehigh.edu.

For technical questions regarding the microscope please contact Bill
Mushock wim5-at-lehigh.edu

--




==============================Original Headers==============================
6, 22 -- From wim5-at-lehigh.edu Wed Feb 15 12:43:09 2006
6, 22 -- Received: from rain.CC.Lehigh.EDU (rain.CC.Lehigh.EDU [128.180.39.20])
6, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1FIh93u029868
6, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Feb 2006 12:43:09 -0600
6, 22 -- Received: from lehigh.edu (Dyn055133.mat.Lehigh.EDU [128.180.55.133])
6, 22 -- (authenticated bits=0)
6, 22 -- by rain.CC.Lehigh.EDU (8.13.5/8.13.5) with ESMTP id k1FIh9Uv018394
6, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
6, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Feb 2006 13:43:09 -0500
6, 22 -- Message-ID: {43F37517.5020702-at-lehigh.edu}
6, 22 -- Date: Wed, 15 Feb 2006 13:38:15 -0500
6, 22 -- From: William J Mushock {wim5-at-lehigh.edu}
6, 22 -- Organization: Lehigh University
6, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax)
6, 22 -- X-Accept-Language: en,en-US
6, 22 -- MIME-Version: 1.0
6, 22 -- To: Microscopy-at-microscopy.com
6, 22 -- Subject: JEOL JSM-6300f FEGSEM for sale
6, 22 -- Content-Type: text/plain; charset=us-ascii; format=flowed
6, 22 -- Content-Transfer-Encoding: 7bit
6, 22 -- X-Virus-Scanned: ClamAV version 0.88, clamav-milter version 0.87 on rain.CC.Lehigh.EDU
6, 22 -- X-Virus-Status: Clean
==============================End of - Headers==============================




From: tonygr-at-MIT.EDU
Date: Wed, 15 Feb 2006 13:26:25 -0600
Subject: [Microscopy] Transfer of used equipment originally bought with US funds

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I'm writing this as a response to Chris Kiely's post, but it is a
very general point that probably bears repeating from time to time.

If a piece of equipment has been purchased by US Government funds
(regardless of agency), then it is not permissible to use other US
Government funds to re-purchase the same piece of equipment
later. This is true even if title to the equipment has been passed
to the holding instrumentation.

To use the specific example of the Lehigh SEM, if it was purchased
through a government grant, I would not be able to buy it from them,
regardless of how much use it would be to me, because the only funds
I have available (at least for the purposes of my illustration!) are
NSF funds. This is a limitation enforced on me, as the spender of
Government funds, rather than on the seller (for they are -
presumably - selling their own property), but it does mean that I
need to know up front whether the instrument was purchased with any
US Government funds (even if other funds were used as well) before I
can decide whether I might be interested in it. I, and my
institution, would be in major hot water should an auditor discover I
had used government funds in this way (I suspect that here at MIT our
internal systems would catch this before the sale went through, but
that may not be true everywhere).

Tony Garratt-Reed.



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA

Tel: 617-253-4622
Fax: 617-258-6478
************************************************


==============================Original Headers==============================
10, 27 -- From tonygr-at-MIT.EDU Wed Feb 15 13:26:25 2006
10, 27 -- Received: from biscayne-one-station.mit.edu (BISCAYNE-ONE-STATION.MIT.EDU [18.7.7.80])
10, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1FJQOI2007557
10, 27 -- for {microscopy-at-microscopy.com} ; Wed, 15 Feb 2006 13:26:25 -0600
10, 27 -- Received: from outgoing.mit.edu (OUTGOING-AUTH.MIT.EDU [18.7.22.103])
10, 27 -- by biscayne-one-station.mit.edu (8.12.4/8.9.2) with ESMTP id k1FJN2lx025785
10, 27 -- for {microscopy-at-microscopy.com} ; Wed, 15 Feb 2006 14:23:02 -0500 (EST)
10, 27 -- Received: from emlab.mit.edu (EMLAB.MIT.EDU [18.82.0.121])
10, 27 -- (authenticated bits=0)
10, 27 -- (User authenticated as tonygr-at-ATHENA.MIT.EDU)
10, 27 -- by outgoing.mit.edu (8.13.1/8.12.4) with ESMTP id k1FJMxC5006737
10, 27 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
10, 27 -- for {microscopy-at-microscopy.com} ; Wed, 15 Feb 2006 14:23:00 -0500 (EST)
10, 27 -- Message-Id: {6.2.3.4.2.20060215140434.01df5468-at-po9.mit.edu}
10, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4
10, 27 -- Date: Wed, 15 Feb 2006 14:22:56 -0500
10, 27 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com}
10, 27 -- From: Anthony Garratt-Reed {tonygr-at-MIT.EDU}
10, 27 -- Subject: Transfer of used equipment originally bought with US funds
10, 27 -- In-Reply-To: {200602151851.k1FIpnQ5006790-at-ns.microscopy.com}
10, 27 -- References: {200602151851.k1FIpnQ5006790-at-ns.microscopy.com}
10, 27 -- Mime-Version: 1.0
10, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
10, 27 -- X-Spam-Score: 1.217
10, 27 -- X-Spam-Level: * (1.217)
10, 27 -- X-Spam-Flag: NO
10, 27 -- X-Scanned-By: MIMEDefang 2.42
==============================End of - Headers==============================




From: zaluzec-at-microscopy.com
Date: Wed, 15 Feb 2006 14:59:46 -0600
Subject: [Microscopy] Administrivia: please read the FAQ

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

Just a reminder to please read the FAQ if you not sure about a posting.

Posting of equipment/items for sale on the Listserver is against our rules.
You should use the MSA Surplus Equipment site for that function.

Nestor
Your Friendly Neighborhood SysOp

==============================Original Headers==============================
4, 12 -- From zaluzec-at-microscopy.com Wed Feb 15 14:59:45 2006
4, 12 -- Received: from [203.98.91.59] (msdvpn29.msd.anl.gov [130.202.238.93])
4, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1FKxbJL019125
4, 12 -- for {microscopy-at-microscopy.com} ; Wed, 15 Feb 2006 14:59:42 -0600
4, 12 -- Mime-Version: 1.0
4, 12 -- X-Sender: zaluzec-at-microscopy.com (Unverified)
4, 12 -- Message-Id: {p06020401c019461f7695-at-[203.98.91.59]}
4, 12 -- Date: Thu, 16 Feb 2006 07:59:36 +1100
4, 12 -- To: microscopy-at-microscopy.com
4, 12 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
4, 12 -- Subject: Administrivia: please read the FAQ
4, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: scanning-at-fams.org
Date: Thu, 16 Feb 2006 10:16:33 -0600
Subject: [Microscopy] SCANNING 2006 Abstract Deadline Extended to March 1

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Please note the following:

ABSTRACT DEADLINE EXTENDED:

Because it's abstract time for both SCANNING 2006 and M&M, we know
there is time pressure on all contributors. We are therefore extending
the abstract deadline for SCANNING 2006 to March 1. Abstracts will
appear in the Program Issue of SCANNING (March-April Issue) if received
by March 1. Thank you.

For current program information and to download the meeting and hotel
registration forms, please visit www.scanning.org.

Best regards,


Phaedra McGuinness
Managing Editor
SCANNING, The Journal of Scanning Microscopies
P.O. Box 485
Mahwah, NJ 07430
Tel: (201) 818-1010 * Fax: (201) 818-0086 *email: scanning-at-fams.org *
Web: www.scanning.org


==============================Original Headers==============================
9, 19 -- From scanning-at-fams.org Thu Feb 16 10:16:33 2006
9, 19 -- Received: from smtp-out1.oct.nac.net (smtp-out1.oct.nac.net [209.123.233.211])
9, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1GGGXMB020450
9, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 16 Feb 2006 10:16:33 -0600
9, 19 -- Received: (qmail 2746 invoked from network); 16 Feb 2006 16:16:32 -0000
9, 19 -- Received: from unknown (HELO mail1.oct.nac.net) (209.123.233.241)
9, 19 -- by smtp-out1.oct.nac.net with SMTP; 16 Feb 2006 11:16:32 -0500
9, 19 -- Received: (qmail 98875 invoked from network); 16 Feb 2006 11:16:32 -0500
9, 19 -- Received: from unknown (HELO ?192.168.2.80?) (64.21.7.106)
9, 19 -- by mail1.oct.nac.net with SMTP; 16 Feb 2006 11:16:32 -0500
9, 19 -- Mime-Version: 1.0 (Apple Message framework v622)
9, 19 -- Content-Transfer-Encoding: 7bit
9, 19 -- Message-Id: {ec57558c3794e12be5ee59db4270ee60-at-fams.org}
9, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
9, 19 -- To: Microscopy List Server Server {Microscopy-at-MSA.Microscopy.Com}
9, 19 -- From: Phaedra McGuinness {scanning-at-fams.org}
9, 19 -- Subject: SCANNING 2006 Abstract Deadline Extended to March 1
9, 19 -- Date: Thu, 16 Feb 2006 11:16:31 -0500
9, 19 -- X-Mailer: Apple Mail (2.622)
==============================End of - Headers==============================




From: grmitch-at-netzero.com
Date: Thu, 16 Feb 2006 14:48:15 -0600
Subject: [Microscopy] AskAMicroscopist: teaching a unit on microscopic life to 5th

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question was submitted to Ask-A-Microscopist by (grmitch-at-netzero.com)
from
http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
on Wednesday, February 15, 2006 at 20:08:01
Remember to consider the Grade/Age of the student when considering the Question
---------------------------------------------------------------------------
Please reply to both grmitch-at-netzero.com as well as to the
Microscopy Listserver
---------------------------------------------------------------------------

Email: grmitch-at-netzero.com
Name: Linda Mitchell

Organization: BPS Environmental Center

Education: K-8 Grade Grammar School

Location: Birmingham, Michigan USA

Title: Microorganisms!

Question: I will be teaching a unit on microscopic life to 5th
graders next month, March (quite a cold month here in Michigan). My
question for you:
What is the best way to keep my microorganisms alive throughout the
program? We obtain our samples directly from the 10 acres of the
property here at the nature center. One of the samples is from our
pond area and the second from the swamp woods area. We have no
difficulty in finding a healthy sample, yet the problem that we often
encounter is keeping them alive. We have supplies - lab pans,
microscopes, even an aquarium that is our "indoor pond" when the
water ices over. Do you have any feeding, climate tips that would
help in these areas? This is a program that is enjoyed by both the
staff and students - they are so amazed by what they find. Thanks for
your input.

---------------------------------------------------------------------------

==============================Original Headers==============================
8, 13 -- From zaluzec-at-microscopy.com Thu Feb 16 14:48:14 2006
8, 13 -- Received: from [203.33.121.155] (msdvpn28.msd.anl.gov [130.202.238.92])
8, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1GKm8MF002242
8, 13 -- for {microscopy-at-microscopy.com} ; Thu, 16 Feb 2006 14:48:12 -0600
8, 13 -- Mime-Version: 1.0
8, 13 -- X-Sender: (Unverified)
8, 13 -- Message-Id: {p06020403c01a95791353-at-[203.33.121.155]}
8, 13 -- Date: Fri, 17 Feb 2006 07:48:06 +1100
8, 13 -- To: microscopy-at-microscopy.com
8, 13 -- From: grmitch-at-netzero.com (by way of Ask-A-Microscopist)
8, 13 -- Subject: AskAMicroscopist: teaching a unit on microscopic life to 5th
8, 13 -- graders
8, 13 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: zaluzec-at-microscopy.com
Date: Thu, 16 Feb 2006 14:52:48 -0600
Subject: [Microscopy] MM2006 Server OverLoad - Deadline extended

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

I'm out of the country right now, but I have been getting multiple
questions about problems with the MM2006 server for submitting
papers.

Please note that there was a HUGE spike in the paper submissions
and the server is overloaded. As a consequence the MM2006
Executive committee has extended the deadline for paper submissions.

A note to this effect is appended below.

Nestor
Your Friendly Neighborhood SysOp (in Australia for the moment).


==================================================


The paper submission deadline for M&M 2006 in Chicago has been
extended two days until 5:00pm Pacific Standard Time on Friday
February 17, 2006. Due to an ongoing problem with the server today we
have decided to extend the deadline to allow everyone the opportunity
to submit their papers. Please try to access the paper submission
site {http://bono.cup.org/} http://bono.cup.org/ later to register and
submit your paper. Our sincerest apologies for any problems this may
have caused.

Thanks,
Paul Kotula
Microscopy &Microanalysis 2006 Program Chair

Paul G. Kotula, Ph.D.
Principal Member of Technical Staff
Materials Characterization Department
Sandia National Laboratories
PO Box 5800, MS 0886
Albuquerque, NM 87185-0886

ph:(505) 844-8947

==============================Original Headers==============================
12, 11 -- From zaluzec-at-microscopy.com Thu Feb 16 14:52:48 2006
12, 11 -- Received: from [203.33.121.155] (msdvpn28.msd.anl.gov [130.202.238.92])
12, 11 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1GKqh4n008778
12, 11 -- for {microscopy-at-microscopy.com} ; Thu, 16 Feb 2006 14:52:45 -0600
12, 11 -- Mime-Version: 1.0
12, 11 -- Message-Id: {p06020404c01a95b6217e-at-[203.33.121.155]}
12, 11 -- Date: Fri, 17 Feb 2006 07:52:41 +1100
12, 11 -- To: microscopy-at-microscopy.com
12, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
12, 11 -- Subject: MM2006 Server OverLoad - Deadline extended
12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: sue.tyler-at-noaa.gov
Date: Thu, 16 Feb 2006 14:56:50 -0600
Subject: [Microscopy] viaWWW: Glass Knife Breaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both sue.tyler-at-noaa.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: sue.tyler-at-noaa.gov
Name: Sue Tyler

Organization: NOAA

Title-Subject: [Filtered] Glass knife breaker

Question: I read the recent string on the glass knife makers and I
was interested in the conclusion. We are interested in purchasing
either the GKM or the Leica KMR. Any pros or cons would be
appreciated.

Regards-
Sue

---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Thu Feb 16 14:56:50 2006
7, 12 -- Received: from [203.33.121.155] (msdvpn28.msd.anl.gov [130.202.238.92])
7, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1GKuifr021053
7, 12 -- for {microscopy-at-microscopy.com} ; Thu, 16 Feb 2006 14:56:47 -0600
7, 12 -- Mime-Version: 1.0
7, 12 -- X-Sender: (Unverified)
7, 12 -- Message-Id: {p0602040cc01a97768aa1-at-[203.33.121.155]}
7, 12 -- Date: Fri, 17 Feb 2006 07:56:42 +1100
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: sue.tyler-at-noaa.gov (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: Glass Knife Breaker
7, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: diller-at-stefan-diller.com
Date: Thu, 16 Feb 2006 14:57:31 -0600
Subject: [Microscopy] viaWWW: TN2000 EDS System

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both diller-at-stefan-diller.com as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: diller-at-stefan-diller.com
Name: Stefan Diller

Organization: Scientific Photography

Title-Subject: [Filtered] Tracor Northern TN2000 EDS system

Question: Dear All,
is anybody out there knowing some details or having some images
available of an 1991 Tracor Northern TN2000 EDS system?

Is there still a possiblity to get service in Germany?
Is there any possibility to get the EDS data out of the analyzer into
a PC for saving, printing, emailing?
What is the latest version of software for the TN2000?

Please reply offline, if convenient...
I will do a summarizing for the list...

Best regards,
Stefan

---------------------------------------------------------------------------

==============================Original Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Thu Feb 16 14:57:30 2006
9, 12 -- Received: from [203.33.121.155] (msdvpn28.msd.anl.gov [130.202.238.92])
9, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1GKvKCm022320
9, 12 -- for {microscopy-at-microscopy.com} ; Thu, 16 Feb 2006 14:57:24 -0600
9, 12 -- Mime-Version: 1.0
9, 12 -- X-Sender: (Unverified)
9, 12 -- Message-Id: {p0602040dc01a97989292-at-[203.33.121.155]}
9, 12 -- Date: Fri, 17 Feb 2006 07:57:17 +1100
9, 12 -- To: microscopy-at-microscopy.com
9, 12 -- From: diller-at-stefan-diller.com (by way of MicroscopyListserver)
9, 12 -- Subject: viaWWW: TN2000 EDS System
9, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: soleimanij-at-tbzmed.ac.ir
Date: Thu, 16 Feb 2006 14:57:52 -0600
Subject: [Microscopy] viaWWW: cutting polymer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both soleimanij-at-tbzmed.ac.ir as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: soleimanij-at-tbzmed.ac.ir
Name: Jafar Soleimani Rad

Organization: University

Title-Subject: [Filtered] cutting a polymer

Question: We are having problem with cutting a polymer, Acrylonitril
Butadien Styrene (ABS.
After embedding in resin it dose not stick to it and becomes
separated when trying to cut with ultramicrotom. It is also
impossible to cut paraffin blocks. I would appreciate if you guide me
with your experiences in this matter.

Sincerely

JSRad


---------------------------------------------------------------------------

==============================Original Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Thu Feb 16 14:57:51 2006
9, 12 -- Received: from [203.33.121.155] (msdvpn28.msd.anl.gov [130.202.238.92])
9, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1GKvk9W023875
9, 12 -- for {microscopy-at-microscopy.com} ; Thu, 16 Feb 2006 14:57:49 -0600
9, 12 -- Mime-Version: 1.0
9, 12 -- X-Sender: (Unverified)
9, 12 -- Message-Id: {p0602040ec01a97b99a3c-at-[203.33.121.155]}
9, 12 -- Date: Fri, 17 Feb 2006 07:57:45 +1100
9, 12 -- To: microscopy-at-microscopy.com
9, 12 -- From: soleimanij-at-tbzmed.ac.ir (by way of MicroscopyListserver)
9, 12 -- Subject: viaWWW: cutting polymer
9, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: b.farwell-at-unf.edu
Date: Thu, 16 Feb 2006 14:59:17 -0600
Subject: [Microscopy] viaWWW: AFM controller connection problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both b.farwell-at-unf.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: b.farwell-at-unf.edu
Name: Brenton

Organization: University of N. Florida (student)

Title-Subject: [Filtered] AFM controller connection problems

Question: We are using a Molecular Imaging AFM and controller and are
using "MI Metrology Series 2000 AFM System" program to interface to
the AFM controller. The computer is having connection issues with
the controller. Cables have been checked, and the setup disk has run
on the controller, it gives 4 beeps, however the computer still can't
connect. Ping gives no response. Anybody have any ideas?
Thanks very much
Brenton

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Thu Feb 16 14:59:17 2006
6, 12 -- Received: from [203.33.121.155] (msdvpn28.msd.anl.gov [130.202.238.92])
6, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1GKxC0D030319
6, 12 -- for {microscopy-at-microscopy.com} ; Thu, 16 Feb 2006 14:59:15 -0600
6, 12 -- Mime-Version: 1.0
6, 12 -- X-Sender: (Unverified)
6, 12 -- Message-Id: {p0602040fc01a980dae0b-at-[203.33.121.155]}
6, 12 -- Date: Fri, 17 Feb 2006 07:59:10 +1100
6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: b.farwell-at-unf.edu (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: AFM controller connection problems
6, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: jcrum-at-ncmir.ucsd.edu
Date: Thu, 16 Feb 2006 15:52:20 -0600
Subject: [Microscopy] Wanted: Balzers MED 010 Manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

I recently acquired a used Balzers MED 010 of unknown vintage. I am in
need of the User Manual, or any such literature. I would like to get
this unit back to operating condition.

Thanks,

John Crum
NCMIR
UCSD

==============================Original Headers==============================
4, 18 -- From jcrum-at-ncmir.ucsd.edu Thu Feb 16 15:52:19 2006
4, 18 -- Received: from ncmir.ucsd.edu (ncmir.ucsd.edu [132.239.16.23])
4, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1GLqIWF027723
4, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Feb 2006 15:52:19 -0600
4, 18 -- Received: from [192.168.10.237] (capisce [132.239.16.109])
4, 18 -- by ncmir.ucsd.edu (8.11.7p1+Sun/8.11.7) with ESMTP id k1GLqIL04880
4, 18 -- for {Microscopy-at-Microscopy.Com} ; Thu, 16 Feb 2006 13:52:18 -0800 (PST)
4, 18 -- Message-ID: {43F4F412.1060004-at-ncmir.ucsd.edu}
4, 18 -- Date: Thu, 16 Feb 2006 13:52:18 -0800
4, 18 -- From: John Crum {jcrum-at-ncmir.ucsd.edu}
4, 18 -- Organization: University of California San Diego CRBS/NCMIR
4, 18 -- User-Agent: Mozilla Thunderbird 1.0.2 (Macintosh/20050317)
4, 18 -- X-Accept-Language: en-us, en
4, 18 -- MIME-Version: 1.0
4, 18 -- To: Microscopy-at-microscopy.com
4, 18 -- Subject: Wanted: Balzers MED 010 Manual
4, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 18 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: andy-at-psemdoctor.com
Date: Thu, 16 Feb 2006 17:20:44 -0600
Subject: [Microscopy] Looking for used PSEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a used PSEM (or parts) to purchase. Preferably this system
would be the original model manufactured by RJ Lee Instruments in the mid to
late 90’s. Anyone that can help me out, please email directly to
andy-at-psemdoctor.com.
Thank you,

Andrew L. Zobel
 
PSEMDOCTOR, LLC
117 Bryant Dr.
Pittsburgh, PA 15235
Tel. 412-215-8906
Fax 412-241-3598
WWW.PSEMDOCTOR.COM




==============================Original Headers==============================
6, 22 -- From andy-at-psemdoctor.com Thu Feb 16 17:20:44 2006
6, 22 -- Received: from rwcrmhc14.comcast.net (rwcrmhc14.comcast.net [204.127.192.84])
6, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1GNKinN025744
6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Feb 2006 17:20:44 -0600
6, 22 -- Received: from azdesktop (c-24-3-48-75.hsd1.pa.comcast.net[24.3.48.75])
6, 22 -- by comcast.net (rwcrmhc14) with SMTP
6, 22 -- id {20060216232043m1400qhsbqe} ; Thu, 16 Feb 2006 23:20:43 +0000
6, 22 -- Reply-To: {andy-at-psemdoctor.com}
6, 22 -- From: "Andrew L. Zobel" {andy-at-psemdoctor.com}
6, 22 -- To: {Microscopy-at-microscopy.com}
6, 22 -- Subject: [Microscopy] Looking for used PSEM
6, 22 -- Date: Thu, 16 Feb 2006 18:20:43 -0500
6, 22 -- Organization: PSEMDOCTOR, LLC
6, 22 -- Message-ID: {000701c6334f$9c923ba0$6501a8c0-at-azdesktop}
6, 22 -- MIME-Version: 1.0
6, 22 -- Content-Type: text/plain;
6, 22 -- charset="iso-8859-1"
6, 22 -- X-Mailer: Microsoft Office Outlook 11
6, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
6, 22 -- Thread-Index: AcYzTv46OmN0PyDUSIGoUkBwqvLP/AAAFumw
6, 22 -- Content-Transfer-Encoding: 8bit
6, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1GNKinN025744
==============================End of - Headers==============================




From: ech-at-interchange.ubc.ca
Date: Thu, 16 Feb 2006 19:56:45 -0600
Subject: [Microscopy] Re: viaWWW: Glass Knife Breaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Sue
We have the Leica glass knife breaker since 2001. We are very happy
with it. Reliable, good knives! Our users like it in preference to
our old workhorse LKB knife breakers.
Elaine

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
Dr. Elaine Humphrey
Director, BioImaging Facility
President, Microscopy Society of Canada (2003-2005)
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-interchange.ubc.ca
website: www.emlab.ubc.ca

==============================Original Headers==============================
4, 29 -- From ech-at-interchange.ubc.ca Thu Feb 16 19:56:45 2006
4, 29 -- Received: from mta1.mail-relay.ubc.ca (mta1.mail-relay.ubc.ca [137.82.45.2])
4, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1H1uiLA004198
4, 29 -- for {microscopy-at-microscopy.com} ; Thu, 16 Feb 2006 19:56:44 -0600
4, 29 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69])
4, 29 -- by mta1.mail-relay.ubc.ca (8.12.11/8.12.11) with ESMTP id k1H1ufaE023680
4, 29 -- for {microscopy-at-microscopy.com} ; Thu, 16 Feb 2006 17:56:41 -0800 (PST)
4, 29 -- (envelope-from ech-at-interchange.ubc.ca)
4, 29 -- Received: from [137.82.85.193] (echpowerbook.emlab.ubc.ca [137.82.85.193])
4, 29 -- by smtp.interchange.ubc.ca
4, 29 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003))
4, 29 -- with ESMTPA id {0IUT00CU86QGQU-at-smtp.interchange.ubc.ca} for
4, 29 -- microscopy-at-microscopy.com; Thu, 16 Feb 2006 17:56:41 -0800 (PST)
4, 29 -- Date: Thu, 16 Feb 2006 17:56:38 -0800
4, 29 -- From: Elaine Humphrey {ech-at-interchange.ubc.ca}
4, 29 -- Subject: Re: [Microscopy] viaWWW: Glass Knife Breaker
4, 29 -- In-reply-to: {200602162058.k1GKw9iM025479-at-ns.microscopy.com}
4, 29 -- X-Sender: ech-at-mail.interchange.ubc.ca
4, 29 -- To: microscopy-at-microscopy.com
4, 29 -- Cc: sue.tyler-at-noaa.gov
4, 29 -- Message-id: {a06100504c01adcc79da1-at-[137.82.85.193]}
4, 29 -- MIME-version: 1.0
4, 29 -- Content-type: text/plain; format=flowed; charset=us-ascii
4, 29 -- References: {200602162058.k1GKw9iM025479-at-ns.microscopy.com}
4, 29 -- X-UBC-Scanned: Sophos PureMessage 4.7.1.128075, Antispam-Engine: 2.1.0.0, Antispam-Data: 2006.02.16.164605
4, 29 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca
4, 29 -- X-PerlMx-Spam: Probability=7%, Report=IP_HTTP_ADDR 0, __C230066_P5 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_CRUFT 0
4, 29 -- X-Spam-Level:
4, 29 -- X-Spam-Flag: No
==============================End of - Headers==============================




From: jeff-at-metallography.com
Date: Fri, 17 Feb 2006 08:43:23 -0600
Subject: [Microscopy] Seeking manual for B&L Research I metallograph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

A colleague is looking for the instruction manual for a Bausch & Lomb
Research I metallograph. If anybody can help him in this quest please
respond directly to him, Gregory Dexter at greg-at-met-sol.com .

Thanks,

Jeff Stewart
Metallographic Laboratory Manager
Stern-Leach Company
49 Pearl Street
Attleboro, MA 02703
508-222-7400 x1329


==============================Original Headers==============================
5, 21 -- From jeff-at-metallography.com Fri Feb 17 08:43:23 2006
5, 21 -- Received: from Q1-ABI-TX.sanimail.com (mail6.mailsystem.us [67.97.234.238])
5, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HEhM02018323
5, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 08:43:23 -0600
5, 21 -- Received: (qmail 68083 invoked by uid 1010); 17 Feb 2006 15:00:57 -0000
5, 21 -- Received: from unknown (HELO jstewart) (4.154.213.130)
5, 21 -- by mail6.mailsystem.us with SMTP; 17 Feb 2006 15:00:57 -0000
5, 21 -- Message-ID: {001801c633d0$9895b500$959610ac-at-sternleach.com}
5, 21 -- Reply-To: "Jeff Stewart" {jeff-at-metallography.com}
5, 21 -- From: "Jeff Stewart" {jeff-at-metallography.com}
5, 21 -- To: {Microscopy-at-microscopy.com}
5, 21 -- Subject: Seeking manual for B&L Research I metallograph
5, 21 -- Date: Fri, 17 Feb 2006 09:41:06 -0500
5, 21 -- MIME-Version: 1.0
5, 21 -- Content-Type: text/plain;
5, 21 -- charset="iso-8859-1"
5, 21 -- Content-Transfer-Encoding: 7bit
5, 21 -- X-Priority: 3
5, 21 -- X-MSMail-Priority: Normal
5, 21 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1409
5, 21 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2800.1409
==============================End of - Headers==============================




From: dsherman-at-purdue.edu
Date: Fri, 17 Feb 2006 11:51:22 -0600
Subject: [Microscopy] Microscope cooling lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

For years we have been wondering where the dark material was coming from
that accumulated in our water filters. These are filters in the closed
circuit lines between our microscopes and water recirculating units. The
lines are mainly in the ceiling or totally insulated as they run down the
walls in the scope rooms. Imaging our surprise when we went to do a minor
repair on one and watched as the plumber removed a regulator and inserted a
piece of galvanized pipe.

Apparently when the building was built (20 years ago), the contractor
used galvanized pipe when copper had been specified. As it was hidden, we
did not know about the switch. All visible lines hooking up the chiller
compressor cooling with building water were copper.

Well now these galvanized lines are really breaking down and clogging the
water pumps. We intend to replace all but were questioning whether it would
be best to replace with copper or PVC piping. Any suggestions?

One concern was whether there would be the need to acid clean these lines in
the future (this is done routinely to the compressor lines to remove mineral
build-up). Since it is closed circuit, we should not accumulate large
amounts of minerals even though tap or deionized water will be used for the
system. We also can control algae growth with chemicals. Any suggestions on
this and should this dictate which material is used for the pipes?

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


==============================Original Headers==============================
8, 21 -- From dsherman-at-purdue.edu Fri Feb 17 11:51:22 2006
8, 21 -- Received: from exchange.purdue.edu (1061exfe02.adpc.purdue.edu [128.210.63.223])
8, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HHpMgE030390
8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 11:51:22 -0600
8, 21 -- Received: from EXCH02.purdue.lcl ([128.210.63.234]) by exchange.purdue.edu with Microsoft SMTPSVC(6.0.3790.211);
8, 21 -- Fri, 17 Feb 2006 12:51:21 -0500
8, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH02.purdue.lcl ([128.210.63.237]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.239]) with Microsoft Exchange Server HTTP-DAV ;
8, 21 -- Fri, 17 Feb 2006 17:51:21 +0000
8, 21 -- User-Agent: Microsoft-Entourage/11.2.1.051004
8, 21 -- Date: Fri, 17 Feb 2006 12:51:20 -0500
8, 21 -- Subject: Microscope cooling lines
8, 21 -- From: Debby Sherman {dsherman-at-purdue.edu}
8, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
8, 21 -- Message-ID: {C01B7748.D5AB%dsherman-at-purdue.edu}
8, 21 -- Thread-Topic: Microscope cooling lines
8, 21 -- Thread-Index: AcYz6sIxAPacp5/eEdqwBAARJN08Mg==
8, 21 -- Mime-version: 1.0
8, 21 -- Content-type: text/plain;
8, 21 -- charset="US-ASCII"
8, 21 -- Content-transfer-encoding: 7bit
8, 21 -- X-OriginalArrivalTime: 17 Feb 2006 17:51:21.0666 (UTC) FILETIME=[C32F5220:01C633EA]
==============================End of - Headers==============================




From: wpchan-at-u.washington.edu
Date: Fri, 17 Feb 2006 12:15:44 -0600
Subject: [Microscopy] manual for Denton DV-502A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi

I wonder if anyone has a manual for the Denton DV-502A vacuum evaporator
that I can borrow. Ours was lost during moving to storage and now I have
to set it up again. It would be nice to make sure I set it up correctly.
Denton can supply the manual for $250 so I am looking at other less
expensive ways first. Thanks a lot!

--
Pang (Wai Pang Chan, wpchan-at-u.washington.edu, PAB A087, 206-685-1519)
The Biology Imaging Facility (http://staff.washington.edu/wpchan/if/)

==============================Original Headers==============================
3, 19 -- From wpchan-at-u.washington.edu Fri Feb 17 12:15:44 2006
3, 19 -- Received: from mxout5.cac.washington.edu (mxout5.cac.washington.edu [140.142.32.135])
3, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HIFhWL007641
3, 19 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 12:15:44 -0600
3, 19 -- Received: from homer24.u.washington.edu (homer24.u.washington.edu [140.142.15.10])
3, 19 -- by mxout5.cac.washington.edu (8.13.5+UW05.10/8.13.5+UW05.09) with ESMTP id k1HIFg5m011897
3, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO)
3, 19 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 10:15:43 -0800
3, 19 -- Received: from localhost (wpchan-at-localhost)
3, 19 -- by homer24.u.washington.edu (8.13.5+UW05.10/8.13.5+Submit) with ESMTP id k1HIFgup027346
3, 19 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 10:15:42 -0800
3, 19 -- Date: Fri, 17 Feb 2006 10:15:42 -0800 (PST)
3, 19 -- From: "W. Chan" {wpchan-at-u.washington.edu}
3, 19 -- To: microscopy-at-microscopy.com
3, 19 -- Subject: manual for Denton DV-502A
3, 19 -- Message-ID: {Pine.LNX.4.64.0602171008220.7757-at-homer24.u.washington.edu}
3, 19 -- MIME-Version: 1.0
3, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
3, 19 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_7 0'
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Fri, 17 Feb 2006 12:57:04 -0600
Subject: [Microscopy] Re: Microscope cooling lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Debby,

For our new IMAGE building, I specified PVC piping for the closed
circ loop between the water chillers and scopes. We still notice a
greenish sludge building up in the water filters (takes about 6-8
months to become significant) but I am certain that this is coming
from the EM (copper cooling coils and iron
connections---} electrolytic reaction). The EM service people told us
that if we ever used acid to clean the lines that they would no
longer warranty the microscope. The PVC lines are perfectly clean.

JB




} For years we have been wondering where the dark material was coming from
} that accumulated in our water filters. These are filters in the closed
} circuit lines between our microscopes and water recirculating units. The
} lines are mainly in the ceiling or totally insulated as they run down the
} walls in the scope rooms. Imaging our surprise when we went to do a minor
} repair on one and watched as the plumber removed a regulator and inserted a
} piece of galvanized pipe.
}
} Apparently when the building was built (20 years ago), the contractor
} used galvanized pipe when copper had been specified. As it was hidden, we
} did not know about the switch. All visible lines hooking up the chiller
} compressor cooling with building water were copper.
}
} Well now these galvanized lines are really breaking down and clogging the
} water pumps. We intend to replace all but were questioning whether it would
} be best to replace with copper or PVC piping. Any suggestions?
}
} One concern was whether there would be the need to acid clean these lines in
} the future (this is done routinely to the compressor lines to remove mineral
} build-up). Since it is closed circuit, we should not accumulate large
} amounts of minerals even though tap or deionized water will be used for the
} system. We also can control algae growth with chemicals. Any suggestions on
} this and should this dictate which material is used for the pipes?
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
} ==============================Original Headers==============================
} 8, 21 -- From dsherman-at-purdue.edu Fri Feb 17 11:51:22 2006
} 8, 21 -- Received: from exchange.purdue.edu
} (1061exfe02.adpc.purdue.edu [128.210.63.223])
} 8, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
} k1HHpMgE030390
} 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006
} 11:51:22 -0600
} 8, 21 -- Received: from EXCH02.purdue.lcl ([128.210.63.234]) by
} exchange.purdue.edu with Microsoft SMTPSVC(6.0.3790.211);
} 8, 21 -- Fri, 17 Feb 2006 12:51:21 -0500
} 8, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by
} EXCH02.purdue.lcl ([128.210.63.237]) via Exchange Front-End Server
} exch.itap.purdue.edu ([128.210.63.239]) with Microsoft Exchange
} Server HTTP-DAV ;
} 8, 21 -- Fri, 17 Feb 2006 17:51:21 +0000
} 8, 21 -- User-Agent: Microsoft-Entourage/11.2.1.051004
} 8, 21 -- Date: Fri, 17 Feb 2006 12:51:20 -0500
} 8, 21 -- Subject: Microscope cooling lines
} 8, 21 -- From: Debby Sherman {dsherman-at-purdue.edu}
} 8, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
} 8, 21 -- Message-ID: {C01B7748.D5AB%dsherman-at-purdue.edu}
} 8, 21 -- Thread-Topic: Microscope cooling lines
} 8, 21 -- Thread-Index: AcYz6sIxAPacp5/eEdqwBAARJN08Mg==
} 8, 21 -- Mime-version: 1.0
} 8, 21 -- Content-type: text/plain;
} 8, 21 -- charset="US-ASCII"
} 8, 21 -- Content-transfer-encoding: 7bit
} 8, 21 -- X-OriginalArrivalTime: 17 Feb 2006 17:51:21.0666 (UTC)
} FILETIME=[C32F5220:01C633EA]
} ==============================End of - Headers==============================


--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################

==============================Original Headers==============================
9, 18 -- From bozzola-at-siu.edu Fri Feb 17 12:57:04 2006
9, 18 -- Received: from abbmta2.siu.edu (abbmta2.siu.edu [131.230.254.206])
9, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HIv3LI017656
9, 18 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 12:57:04 -0600
9, 18 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142])
9, 18 -- by abbmta2.siu.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id k1HIv252005395
9, 18 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 12:57:03 -0600 (CST)
9, 18 -- Mime-Version: 1.0
9, 18 -- X-Sender: bozzola-at-saluki-mail.siu.edu
9, 18 -- Message-Id: {p0611040bc01bcb431cdd-at-[131.230.177.142]}
9, 18 -- In-Reply-To: {200602171754.k1HHsAVw032278-at-ns.microscopy.com}
9, 18 -- References: {200602171754.k1HHsAVw032278-at-ns.microscopy.com}
9, 18 -- Date: Fri, 17 Feb 2006 12:57:00 -0600
9, 18 -- To: Microscopy-at-msa.microscopy.com
9, 18 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
9, 18 -- Subject: Re: [Microscopy] Microscope cooling lines
9, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
9, 18 -- X-MASF: 0.00%
==============================End of - Headers==============================




From: pgrover-at-bilbo.bio.purdue.edu
Date: Fri, 17 Feb 2006 13:19:13 -0600
Subject: [Microscopy] re: microscope cooling lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Debby,

Another material you may wish to consider is called PEX, which is a
cross-linked polyethylene. I don't know too much about its characteristics,
except that it's very smooth inside, which should retard crud accumulation
and it's more opaque than white pvc. It may be worth checking out.

Paul


--------------------------------------------------------------------------
Famous Last Words Department: "I did not get my Spaghetti-O's, I got
spaghetti. I want the press to know this."
~~ Thomas J. Grasso, d. March 20, 1995 Executed by injection, Oklahoma.



==============================Original Headers==============================
7, 21 -- From pgrover-at-bilbo.bio.purdue.edu Fri Feb 17 13:19:13 2006
7, 21 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128])
7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HJJCk8027344
7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 13:19:12 -0600
7, 21 -- Received: from paklabpgrover (dhcp155-122.bio.purdue.edu [128.210.155.122])
7, 21 -- by mailhub128.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id k1HJJCLp010612
7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 14:19:12 -0500
7, 21 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu}
7, 21 -- To: {microscopy-at-microscopy.com}
7, 21 -- Subject: re: microscope cooling lines
7, 21 -- Date: Fri, 17 Feb 2006 14:19:17 -0500
7, 21 -- Message-ID: {000301c633f7$0bb24910$7a9bd280-at-paklabpgrover}
7, 21 -- MIME-Version: 1.0
7, 21 -- Content-Type: text/plain;
7, 21 -- charset="us-ascii"
7, 21 -- Content-Transfer-Encoding: 7bit
7, 21 -- X-Mailer: Microsoft Office Outlook 11
7, 21 -- Thread-Index: AcYz9wt9iKTkG2SVRtGWvOlC3m8itw==
7, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
7, 21 -- X-PMX-Version: 4.7.1.128075
7, 21 -- X-PerlMx-Virus-Scanned: Yes
==============================End of - Headers==============================




From: dljones-at-bestweb.net
Date: Fri, 17 Feb 2006 13:24:27 -0600
Subject: [Microscopy] Re: Microscope cooling lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Debbie,

It would be good to know what is the operating temperature, pressure, flow rate
and the characteristics of the water feed for make-up.

There is no material in-compatibility between copper piping and PVC piping. They
can be used in the same water curcuit.

If you want to know if there is a problem using copper, you can take some of the
water out of the system and see how much copper is present. You need to have a
base line of copper from the water source, so you should take a water sample
from the source also. There is likely not a problem, it depends upon your water
chemistry. Knowing your water chemistry is fundamental to knowing what piping is
preferred.

You may have building code restrictions regarding PVC piping that is hidden,
which may be the reason the orginal contractor put in galvanized piping. You
will have to look at what codes apply to where you are.

Regarding acid cleaning, you should know what your deposits are in your piping
before deciding how to clean them. As an FYI, copper will generally corrode at
pH's below about 6.3 or so. There are low pH cleaners that can be used with
copper, but they contain corrosion inhibitors.

dj

On Fri, 17 Feb 2006 dsherman-at-purdue.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Listers,
}
} For years we have been wondering where the dark material was coming from
} that accumulated in our water filters. These are filters in the closed
} circuit lines between our microscopes and water recirculating units. The
} lines are mainly in the ceiling or totally insulated as they run down the
} walls in the scope rooms. Imaging our surprise when we went to do a minor
} repair on one and watched as the plumber removed a regulator and inserted a
} piece of galvanized pipe.
}
} Apparently when the building was built (20 years ago), the contractor
} used galvanized pipe when copper had been specified. As it was hidden, we
} did not know about the switch. All visible lines hooking up the chiller
} compressor cooling with building water were copper.
}
} Well now these galvanized lines are really breaking down and clogging the
} water pumps. We intend to replace all but were questioning whether it would
} be best to replace with copper or PVC piping. Any suggestions?
}
} One concern was whether there would be the need to acid clean these lines in
} the future (this is done routinely to the compressor lines to remove mineral
} build-up). Since it is closed circuit, we should not accumulate large
} amounts of minerals even though tap or deionized water will be used for the
} system. We also can control algae growth with chemicals. Any suggestions on
} this and should this dictate which material is used for the pipes?
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
} ==============================Original Headers==============================
} 8, 21 -- From dsherman-at-purdue.edu Fri Feb 17 11:51:22 2006
} 8, 21 -- Received: from exchange.purdue.edu (1061exfe02.adpc.purdue.edu [128.210.63.223])
} 8, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HHpMgE030390
} 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 11:51:22 -0600
} 8, 21 -- Received: from EXCH02.purdue.lcl ([128.210.63.234]) by exchange.purdue.edu with Microsoft SMTPSVC(6.0.3790.211);
} 8, 21 -- Fri, 17 Feb 2006 12:51:21 -0500
} 8, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH02.purdue.lcl ([128.210.63.237]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.239]) with Microsoft Exchange Server HTTP-DAV ;
} 8, 21 -- Fri, 17 Feb 2006 17:51:21 +0000
} 8, 21 -- User-Agent: Microsoft-Entourage/11.2.1.051004
} 8, 21 -- Date: Fri, 17 Feb 2006 12:51:20 -0500
} 8, 21 -- Subject: Microscope cooling lines
} 8, 21 -- From: Debby Sherman {dsherman-at-purdue.edu}
} 8, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
} 8, 21 -- Message-ID: {C01B7748.D5AB%dsherman-at-purdue.edu}
} 8, 21 -- Thread-Topic: Microscope cooling lines
} 8, 21 -- Thread-Index: AcYz6sIxAPacp5/eEdqwBAARJN08Mg==
} 8, 21 -- Mime-version: 1.0
} 8, 21 -- Content-type: text/plain;
} 8, 21 -- charset="US-ASCII"
} 8, 21 -- Content-transfer-encoding: 7bit
} 8, 21 -- X-OriginalArrivalTime: 17 Feb 2006 17:51:21.0666 (UTC) FILETIME=[C32F5220:01C633EA]
} ==============================End of - Headers==============================
}


==============================Original Headers==============================
10, 25 -- From dljones-at-bestweb.net Fri Feb 17 13:24:26 2006
10, 25 -- Received: from smtp2.bestweb.net (smtp2-2.bestweb.net [209.94.103.46])
10, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HJOOlm003358
10, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 13:24:26 -0600
10, 25 -- Received: from smtp2.bestweb.net (localhost [127.0.0.1])
10, 25 -- by smtp2.bestweb.net (Postfix) with ESMTP id 747541CD06;
10, 25 -- Fri, 17 Feb 2006 14:24:23 -0500 (EST)
10, 25 -- Received: from dlj (pool-71-249-9-96.nycmny.east.verizon.net [71.249.9.96])
10, 25 -- by smtp2.bestweb.net (Postfix) with ESMTP id CAD7C1CCC2;
10, 25 -- Fri, 17 Feb 2006 14:24:21 -0500 (EST)
10, 25 -- Date: Fri, 17 Feb 2006 14:33:39 -0500 (Eastern Standard Time)
10, 25 -- From: "David L. Jones" {dljones-at-bestweb.net}
10, 25 -- To: dsherman-at-purdue.edu
10, 25 -- cc: Microscopy-at-microscopy.com
10, 25 -- Subject: Re: [Microscopy] Microscope cooling lines
10, 25 -- In-Reply-To: {200602171802.k1HI2DeL005131-at-ns.microscopy.com}
10, 25 -- Message-ID: {Pine.WNT.4.62.0602171405030.1080-at-dlj}
10, 25 -- References: {200602171802.k1HI2DeL005131-at-ns.microscopy.com}
10, 25 -- X-X-Sender: dljones-at-pop-croton.bestweb.net
10, 25 -- MIME-Version: 1.0
10, 25 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
10, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.2 (2004-11-16) on smtp2-1.bestweb.net
10, 25 -- X-Spam-Level:
10, 25 -- X-Spam-Status: No, score=-2.8 required=5.0 tests=ALL_TRUSTED autolearn=failed
10, 25 -- version=3.0.2
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Fri, 17 Feb 2006 13:36:47 -0600
Subject: [Microscopy] Re: Microscope cooling lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Feb 17, 2006, at 9:51 AM, dsherman-at-purdue.edu wrote:

} For years we have been wondering where the dark material was coming
} from
} that accumulated in our water filters. These are filters in the closed
} circuit lines between our microscopes and water recirculating units.
} The
} lines are mainly in the ceiling or totally insulated as they run down
} the
} walls in the scope rooms. Imaging our surprise when we went to do a
} minor
} repair on one and watched as the plumber removed a regulator and
} inserted a
} piece of galvanized pipe.
}
} Apparently when the building was built (20 years ago), the
} contractor
} used galvanized pipe when copper had been specified. As it was
} hidden, we
} did not know about the switch. All visible lines hooking up the
} chiller
} compressor cooling with building water were copper.
}
} Well now these galvanized lines are really breaking down and clogging
} the
} water pumps. We intend to replace all but were questioning whether it
} would
} be best to replace with copper or PVC piping. Any suggestions?
}
} One concern was whether there would be the need to acid clean these
} lines in
} the future (this is done routinely to the compressor lines to remove
} mineral
} build-up). Since it is closed circuit, we should not accumulate large
} amounts of minerals even though tap or deionized water will be used
} for the
} system. We also can control algae growth with chemicals. Any
} suggestions on
} this and should this dictate which material is used for the pipes?
}
Dear Debby,
If you intend to clean the lines with acid, I suggest PVC, since Cu
can be etched at low pH. In addition to floating some dichlorophene
for algal control, we add a corrosion inhibitor. We have been using a
Mo-based formula, which was available from Aqua Labs on the East coast
and from Skasol on the West coast, so find a distributor in your area.
I think that either Aqua or Skasol would be able to give you that info.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
4, 22 -- From tivol-at-caltech.edu Fri Feb 17 13:36:46 2006
4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HJakQG013933
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 13:36:46 -0600
4, 22 -- Received: from localhost (earth-dog [192.168.1.3])
4, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id 040B535C5A
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 11:36:46 -0800 (PST)
4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21])
4, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id AC201340D7
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 11:36:43 -0800 (PST)
4, 22 -- Mime-Version: 1.0 (Apple Message framework v623)
4, 22 -- In-Reply-To: {200602171751.k1HHpVq9030559-at-ns.microscopy.com}
4, 22 -- References: {200602171751.k1HHpVq9030559-at-ns.microscopy.com}
4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
4, 22 -- Message-Id: {6f18d2977f3ac75cf6e6159ba16304e9-at-caltech.edu}
4, 22 -- Content-Transfer-Encoding: 7bit
4, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
4, 22 -- Subject: Re: [Microscopy] Microscope cooling lines
4, 22 -- Date: Fri, 17 Feb 2006 11:44:38 -0800
4, 22 -- To: microscopy-at-msa.microscopy.com
4, 22 -- X-Mailer: Apple Mail (2.623)
4, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.3.3
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Fri, 17 Feb 2006 13:42:58 -0600
Subject: [Microscopy] microscope cooling lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Feb 17, 2006, at 11:19 AM, pgrover-at-bilbo.bio.purdue.edu wrote:

} Another material you may wish to consider is called PEX, which is a
} cross-linked polyethylene. I don't know too much about its
} characteristics,
} except that it's very smooth inside, which should retard crud
} accumulation
} and it's more opaque than white pvc. It may be worth checking out.
}
Dear Paul,
PEX sounds like a better material than PVC. It should be essentially
inert--I expect that it will withstand concentrated bases and acids and
would be unaffected by organic solvents (not that one would want to run
those through the scope lines).
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
4, 22 -- From tivol-at-caltech.edu Fri Feb 17 13:42:57 2006
4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HJgvr6022901
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 13:42:57 -0600
4, 22 -- Received: from localhost (earth-dog [192.168.1.3])
4, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id 28C8235764
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 11:42:57 -0800 (PST)
4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21])
4, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id 4558E340D7
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 11:42:56 -0800 (PST)
4, 22 -- Mime-Version: 1.0 (Apple Message framework v623)
4, 22 -- In-Reply-To: {200602171919.k1HJJIaZ027514-at-ns.microscopy.com}
4, 22 -- References: {200602171919.k1HJJIaZ027514-at-ns.microscopy.com}
4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
4, 22 -- Message-Id: {929b43e7c910fd8036e0032d59e72528-at-caltech.edu}
4, 22 -- Content-Transfer-Encoding: 7bit
4, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
4, 22 -- Subject: Re: [Microscopy] re: microscope cooling lines
4, 22 -- Date: Fri, 17 Feb 2006 11:50:51 -0800
4, 22 -- To: microscopy-at-msa.microscopy.com
4, 22 -- X-Mailer: Apple Mail (2.623)
4, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.3.3
==============================End of - Headers==============================




From: dljones-at-bestweb.net
Date: Fri, 17 Feb 2006 13:43:51 -0600
Subject: [Microscopy] Microscope cooling lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

JB,

As an FYI, if you have copper based and iron based materials mixed in the same
system, the iron will corrode preferentially through galvanic coupling, not the
copper. In fact, the iron becomes a sacrifical anode protecting the copper from
corrosion. Any time you have those two materials in the same system, you should
have dielectric couplings between the two or you will actively corrode the
ferrous based material.

If you are having a copper corrosion problem, you should be looking elsewhere
for the cause...

dj

On Fri, 17 Feb 2006 bozzola-at-siu.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Debby,
}
} For our new IMAGE building, I specified PVC piping for the closed
} circ loop between the water chillers and scopes. We still notice a
} greenish sludge building up in the water filters (takes about 6-8
} months to become significant) but I am certain that this is coming
} from the EM (copper cooling coils and iron
} connections---} electrolytic reaction). The EM service people told us
} that if we ever used acid to clean the lines that they would no
} longer warranty the microscope. The PVC lines are perfectly clean.
}
} JB
}


==============================Original Headers==============================
7, 25 -- From dljones-at-bestweb.net Fri Feb 17 13:43:51 2006
7, 25 -- Received: from smtp2.bestweb.net (smtp2-2.bestweb.net [209.94.103.46])
7, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HJhoE0024591
7, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 13:43:51 -0600
7, 25 -- Received: from smtp2.bestweb.net (localhost [127.0.0.1])
7, 25 -- by smtp2.bestweb.net (Postfix) with ESMTP id EDFB81CD04;
7, 25 -- Fri, 17 Feb 2006 14:43:49 -0500 (EST)
7, 25 -- Received: from dlj (pool-71-249-9-96.nycmny.east.verizon.net [71.249.9.96])
7, 25 -- by smtp2.bestweb.net (Postfix) with ESMTP id 7E4831CCC2;
7, 25 -- Fri, 17 Feb 2006 14:43:48 -0500 (EST)
7, 25 -- Date: Fri, 17 Feb 2006 14:53:06 -0500 (Eastern Standard Time)
7, 25 -- From: "David L. Jones" {dljones-at-bestweb.net}
7, 25 -- To: bozzola-at-siu.edu
7, 25 -- cc: Microscopy-at-microscopy.com
7, 25 -- Subject: Re: [Microscopy] Re: Microscope cooling lines
7, 25 -- In-Reply-To: {200602171902.k1HJ2UNa024787-at-ns.microscopy.com}
7, 25 -- Message-ID: {Pine.WNT.4.62.0602171446420.1080-at-dlj}
7, 25 -- References: {200602171902.k1HJ2UNa024787-at-ns.microscopy.com}
7, 25 -- X-X-Sender: dljones-at-pop-croton.bestweb.net
7, 25 -- MIME-Version: 1.0
7, 25 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
7, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.2 (2004-11-16) on smtp2-1.bestweb.net
7, 25 -- X-Spam-Level:
7, 25 -- X-Spam-Status: No, score=-2.8 required=5.0 tests=ALL_TRUSTED autolearn=failed
7, 25 -- version=3.0.2
==============================End of - Headers==============================




From: jecalvin-at-vassar.edu
Date: Fri, 17 Feb 2006 14:40:00 -0600
Subject: [Microscopy] Re: Microscope cooling lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Along these lines, I noticed a lot more copper leaching into the
lines when I used deionized water than regular or filtered tap water.

Secondly, Why hasn't anyone suggested using antifreeze? Antifreeze
intended for cars should have corrosion inhibitors and should be
suitable for this purpose.

I have still gotten green particles regardless of whether antifreeze
was used or not and have regularly made it a practice to clean the
filters in the lines once a semester. Jerry Calvin



At 1:46 PM -0600 2/17/06, dljones-at-bestweb.net wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
****************************
Jerry G. Calvin
Science Support Technician
Box 0731 Biology Department
Vassar College
124 Raymond Avenue
Poughkeepsie, NY 12604-0731

(845) 437-7423 - Office
(845) 437-7424 - Confocal Room
FAX: (845) 437-7315
E-Mail: jecalvin-at-vassar.edu

==============================Original Headers==============================
9, 22 -- From jecalvin-at-vassar.edu Fri Feb 17 14:39:59 2006
9, 22 -- Received: from ironport02.vassar.edu (ironport02.vassar.edu [143.229.1.219])
9, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HKdxB5010878
9, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 14:39:59 -0600
9, 22 -- Received: from smtp01.vassar.edu ([143.229.1.141])
9, 22 -- by ironport02.vassar.edu with ESMTP; 17 Feb 2006 15:39:58 -0500
9, 22 -- X-IronPort-AV: i="4.02,124,1139202000";
9, 22 -- d="scan'208"; a="1488007:sNHT61685337"
9, 22 -- Received: from [143.229.41.193] (unknown [143.229.41.193])
9, 22 -- by smtp01.vassar.edu (Postfix) with ESMTP id 511702E594;
9, 22 -- Fri, 17 Feb 2006 14:34:27 -0500 (EST)
9, 22 -- Mime-Version: 1.0
9, 22 -- X-Sender: jecalvin-at-pop.vassar.edu
9, 22 -- Message-Id: {a06001203c01bde1ab81a-at-[143.229.41.193]}
9, 22 -- In-Reply-To: {200602171946.k1HJkq7E000347-at-ns.microscopy.com}
9, 22 -- References: {200602171946.k1HJkq7E000347-at-ns.microscopy.com}
9, 22 -- Date: Fri, 17 Feb 2006 15:39:58 -0500
9, 22 -- To: dljones-at-bestweb.net
9, 22 -- From: Jerry Calvin {jecalvin-at-vassar.edu}
9, 22 -- Subject: Re: [Microscopy] Microscope cooling lines
9, 22 -- Cc: Microscopy-at-microscopy.com
9, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: davilla-at-4pi.com
Date: Fri, 17 Feb 2006 15:17:27 -0600
Subject: [Microscopy] Re: microscope cooling lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

} PEX sounds like a better material than PVC. It should be essentially

Beware that PEX will degrade over time in sunlight (ultraviolet).
That said, I have PEX in my house instead of Cu, works well and is
easy to run, however all transitions through the walls are Cu
fittings so the PEX stays in the dark.

Scott
--
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com


==============================Original Headers==============================
4, 16 -- From davilla-at-4pi.com Fri Feb 17 15:17:27 2006
4, 16 -- Received: from mx.4pi.com (mx.4pi.com [24.172.19.59])
4, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1HLHQjh021101
4, 16 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 15:17:27 -0600
4, 16 -- Received: from [24.172.19.62] (HELO [192.168.9.10])
4, 16 -- by mx.4pi.com (Stalker SMTP Server 1.8b9d14)
4, 16 -- with ESMTP id S.0000850373 for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 16:17:26 -0500
4, 16 -- Mime-Version: 1.0
4, 16 -- Message-Id: {p06230957c01bece6d048-at-[192.168.9.10]}
4, 16 -- In-Reply-To: {200602171943.k1HJh1eC023022-at-ns.microscopy.com}
4, 16 -- References: {200602171943.k1HJh1eC023022-at-ns.microscopy.com}
4, 16 -- Date: Fri, 17 Feb 2006 16:17:38 -0500
4, 16 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com}
4, 16 -- From: "Scott D. Davilla" {davilla-at-4pi.com}
4, 16 -- Subject: Re: [Microscopy] microscope cooling lines
4, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: dljones-at-bestweb.net
Date: Fri, 17 Feb 2006 15:21:32 -0600
Subject: [Microscopy] Re: Microscope cooling lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Jerry,

Yes, it is not unusual to find more copper leaching into the lines with
deionized water than tap water. It does depend upon the tap water chemistry.

I would not recommend antifreeze for cars, however, I would recommend to use
HVAC grade propylene glycol. That would include inhibitors to protect the metals
in the system and it includes various stablizers for the glycol. I guess if
there are glycols for cars that are propylene glycol based with inhibitors,
those may be OK. I most certainly would not use ethylene glycol based
antifreeze.

I would ask you what kind of antifreeze you are using and have you done an EDS
spectrum of your green deposit from you filters? I would think further
discussion of your specifics may best be addressed off the list...

dj

On Fri, 17 Feb 2006, Jerry Calvin wrote:

} Along these lines, I noticed a lot more copper leaching into the lines when
} I used deionized water than regular or filtered tap water.
}
} Secondly, Why hasn't anyone suggested using antifreeze? Antifreeze intended
} for cars should have corrosion inhibitors and should be suitable for this
} purpose.
}
} I have still gotten green particles regardless of whether antifreeze was used
} or not and have regularly made it a practice to clean the filters in the
} lines once a semester. Jerry Calvin
}
}
}
} At 1:46 PM -0600 2/17/06, dljones-at-bestweb.net wrote:
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } JB,
} }
} } As an FYI, if you have copper based and iron based materials mixed in the
} } same
} } system, the iron will corrode preferentially through galvanic coupling, not
} } the
} } copper. In fact, the iron becomes a sacrifical anode protecting the copper
} } from
} } corrosion. Any time you have those two materials in the same system, you
} } should
} } have dielectric couplings between the two or you will actively corrode the
} } ferrous based material.
} }
} } If you are having a copper corrosion problem, you should be looking
} } elsewhere
} } for the cause...
} }
} } dj
} }
} } On Fri, 17 Feb 2006 bozzola-at-siu.edu wrote:
} }
} } }
} } }
} } }
} } } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } } America
} } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ----------------------------------------------------------------------------
} } }
} } } Debby,
} } }
} } } For our new IMAGE building, I specified PVC piping for the closed
} } } circ loop between the water chillers and scopes. We still notice a
} } } greenish sludge building up in the water filters (takes about 6-8
} } } months to become significant) but I am certain that this is coming
} } } from the EM (copper cooling coils and iron
} } } connections---} electrolytic reaction). The EM service people told us
} } } that if we ever used acid to clean the lines that they would no
} } } longer warranty the microscope. The PVC lines are perfectly clean.
} } }
} } } JB
} } }
} }
} }
} } ==============================Original
} } Headers==============================
} } 7, 25 -- From dljones-at-bestweb.net Fri Feb 17 13:43:51 2006
} } 7, 25 -- Received: from smtp2.bestweb.net (smtp2-2.bestweb.net
} } [209.94.103.46])
} } 7, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
} } k1HJhoE0024591
} } 7, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 13:43:51
} } -0600
} } 7, 25 -- Received: from smtp2.bestweb.net (localhost [127.0.0.1])
} } 7, 25 -- by smtp2.bestweb.net (Postfix) with ESMTP id EDFB81CD04;
} } 7, 25 -- Fri, 17 Feb 2006 14:43:49 -0500 (EST)
} } 7, 25 -- Received: from dlj (pool-71-249-9-96.nycmny.east.verizon.net
} } [71.249.9.96])
} } 7, 25 -- by smtp2.bestweb.net (Postfix) with ESMTP id 7E4831CCC2;
} } 7, 25 -- Fri, 17 Feb 2006 14:43:48 -0500 (EST)
} } 7, 25 -- Date: Fri, 17 Feb 2006 14:53:06 -0500 (Eastern Standard Time)
} } 7, 25 -- From: "David L. Jones" {dljones-at-bestweb.net}
} } 7, 25 -- To: bozzola-at-siu.edu
} } 7, 25 -- cc: Microscopy-at-microscopy.com
} } 7, 25 -- Subject: Re: [Microscopy] Re: Microscope cooling lines
} } 7, 25 -- In-Reply-To: {200602171902.k1HJ2UNa024787-at-ns.microscopy.com}
} } 7, 25 -- Message-ID: {Pine.WNT.4.62.0602171446420.1080-at-dlj}
} } 7, 25 -- References: {200602171902.k1HJ2UNa024787-at-ns.microscopy.com}
} } 7, 25 -- X-X-Sender: dljones-at-pop-croton.bestweb.net
} } 7, 25 -- MIME-Version: 1.0
} } 7, 25 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
} } 7, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.2 (2004-11-16) on
} } smtp2-1.bestweb.net
} } 7, 25 -- X-Spam-Level:
} } 7, 25 -- X-Spam-Status: No, score=-2.8 required=5.0 tests=ALL_TRUSTED
} } autolearn=failed
} } 7, 25 -- version=3.0.2
} } ==============================End of -
} } Headers==============================
}
}
} --
} ****************************
} Jerry G. Calvin
} Science Support Technician
} Box 0731 Biology Department
} Vassar College
} 124 Raymond Avenue
} Poughkeepsie, NY 12604-0731
}
} (845) 437-7423 - Office
} (845) 437-7424 - Confocal Room
} FAX: (845) 437-7315
} E-Mail: jecalvin-at-vassar.edu
}


==============================Original Headers==============================
8, 26 -- From dljones-at-bestweb.net Fri Feb 17 15:21:32 2006
8, 26 -- Received: from smtp2.bestweb.net (smtp2-2.bestweb.net [209.94.103.46])
8, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HLLVkH026422
8, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 15:21:31 -0600
8, 26 -- Received: from smtp2.bestweb.net (localhost [127.0.0.1])
8, 26 -- by smtp2.bestweb.net (Postfix) with ESMTP id C09831CCF1;
8, 26 -- Fri, 17 Feb 2006 16:21:30 -0500 (EST)
8, 26 -- Received: from dlj (pool-71-249-9-96.nycmny.east.verizon.net [71.249.9.96])
8, 26 -- by smtp2.bestweb.net (Postfix) with ESMTP id 27CFB1CCE0;
8, 26 -- Fri, 17 Feb 2006 16:21:30 -0500 (EST)
8, 26 -- Date: Fri, 17 Feb 2006 16:30:47 -0500 (Eastern Standard Time)
8, 26 -- From: "David L. Jones" {dljones-at-bestweb.net}
8, 26 -- To: Jerry Calvin {jecalvin-at-vassar.edu}
8, 26 -- cc: Microscopy-at-microscopy.com
8, 26 -- Subject: Re: [Microscopy] Microscope cooling lines
8, 26 -- In-Reply-To: {a06001203c01bde1ab81a-at-[143.229.41.193]}
8, 26 -- Message-ID: {Pine.WNT.4.62.0602171608170.1080-at-dlj}
8, 26 -- References: {200602171946.k1HJkq7E000347-at-ns.microscopy.com}
8, 26 -- {a06001203c01bde1ab81a-at-[143.229.41.193]}
8, 26 -- X-X-Sender: dljones-at-pop-croton.bestweb.net
8, 26 -- MIME-Version: 1.0
8, 26 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
8, 26 -- X-Spam-Checker-Version: SpamAssassin 3.0.2 (2004-11-16) on smtp2-1.bestweb.net
8, 26 -- X-Spam-Level:
8, 26 -- X-Spam-Status: No, score=-2.8 required=5.0 tests=ALL_TRUSTED autolearn=failed
8, 26 -- version=3.0.2
==============================End of - Headers==============================




From: bagnell-at-med.unc.edu
Date: Fri, 17 Feb 2006 16:01:57 -0600
Subject: [Microscopy] Floaters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

A colleague asked me why she is so much more aware of the
floaters in her eyes when she is using the microscope (or the
telescope) than at other times. I assured her that it wasn't just
her, it happens to a lot of us. But why floaters are so much more
noticeable when looking in the microscope I do not know? Any
suggestions?

Bob
--
C. Robert Bagnell, Jr., Ph.D.
Professor and
Director, Microscopy Services Laboratory
Department of Pathology and Laboratory Medicine
University of North Carolina at Chapel Hill
Chapel Hill, NC 27599
phone 919-966-2413
fax 919-966-6718
e-mail bagnell-at-med.unc.edu
web http://www.med.unc.edu/microscopy

==============================Original Headers==============================
2, 21 -- From bagnell-at-med.unc.edu Fri Feb 17 16:01:56 2006
2, 21 -- Received: from smtp-mx.med.unc.edu (fletcher.med.unc.edu [152.19.4.46])
2, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HM1u7m008604
2, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 16:01:56 -0600
2, 21 -- Received: from castlehayne.med.unc.edu (castlehayne.med.unc.edu [152.19.4.29])
2, 21 -- by smtp-mx.med.unc.edu (8.13.4+Sun/8.13.4) with ESMTP id k1HM1qc1025249
2, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 17:01:56 -0500 (EST)
2, 21 -- Received: from [152.19.80.19] by msrv0.med.unc.edu
2, 21 -- (Sun Java System Messaging Server 6.2-5.02 (built Dec 1 2005))
2, 21 -- with ESMTPA id {0IUU00C9DQJ236B0-at-msrv0.med.unc.edu} for
2, 21 -- microscopy-at-microscopy.com; Fri, 17 Feb 2006 17:01:51 -0500 (EST)
2, 21 -- Date: Fri, 17 Feb 2006 17:01:49 -0500
2, 21 -- From: Robert Bagnell {bagnell-at-med.unc.edu}
2, 21 -- Subject: Floaters
2, 21 -- X-Sender: rml-at-imap-ns.med.unc.edu
2, 21 -- To: microscopy-at-microscopy.com
2, 21 -- Message-id: {p06110400c01bdabc3b81-at-[152.19.80.19]}
2, 21 -- MIME-version: 1.0
2, 21 -- Content-type: text/plain; charset=us-ascii; format=flowed
2, 21 -- Content-transfer-encoding: 7BIT
2, 21 -- X-Scanned-By: UNC/SoM/OIS/Mail_Filter on 152.19.4.46
==============================End of - Headers==============================




From: jfactor-at-ns.purchase.edu
Date: Fri, 17 Feb 2006 16:02:30 -0600
Subject: [Microscopy] Re: Microscope cooling lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

My service engineer recommends neither tap nor distilled water, but
rather bottled spring water. Has anyone yet mentioned the possibility
that green sludge in the filter might be algae growing in the cooling
water or the cooling unit?
--Jan Factor

---------------------------------------
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College, State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------



==============================Original Headers==============================
4, 16 -- From jfactor-at-ns.purchase.edu Fri Feb 17 16:02:30 2006
4, 16 -- Received: from zephyr.ns.purchase.edu (zephyr.ns.purchase.edu [199.79.168.193])
4, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HM2PAx009529
4, 16 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 16:02:25 -0600
4, 16 -- Received: from [10.52.0.79] ([10.52.0.79])
4, 16 -- by zephyr.ns.purchase.edu (AIX5.1/8.11.6p2/8.11.0) with ESMTP id k1HM5lr17594;
4, 16 -- Fri, 17 Feb 2006 17:05:48 -0500
4, 16 -- Message-ID: {43F647F0.1070409-at-ns.purchase.edu}
4, 16 -- Date: Fri, 17 Feb 2006 17:02:24 -0500
4, 16 -- From: Jan Factor {jfactor-at-ns.purchase.edu}
4, 16 -- User-Agent: Thunderbird 1.5 (Windows/20051201)
4, 16 -- MIME-Version: 1.0
4, 16 -- To: microscopy-at-microscopy.com
4, 16 -- Subject: Re: Microscope cooling lines
4, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 16 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: leis-at-biochem.mpg.de
Date: Fri, 17 Feb 2006 16:07:44 -0600
Subject: [Microscopy] viaWWW: microtome section thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both leis-at-biochem.mpg.de as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: leis-at-biochem.mpg.de
Name: Andrew Leis

Organization: MPI for Biochemistry

Title-Subject: [Filtered] microtome section thickness

Question: Can anybody provide an explanation concerning the
refractive index -dependency of the interference colours seen in
charts for estimating the thickness of ultrathin sections? The small
print in such charts usually reads "valid for refractive index of ca.
1.5", which is fine for methacrylates and similar embedding media,
but what type of shift (if significant) would be observed for a lower
refractive index, say 1.3? I am not sure whether the usual charts are
valid for the interference colours given by cryosections.

Any assistance would be appreciated.

---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Fri Feb 17 16:07:44 2006
7, 12 -- Received: from [203.98.91.208] (msdvpn25.msd.anl.gov [130.202.238.89])
7, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HM7b5n021850
7, 12 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 16:07:39 -0600
7, 12 -- Mime-Version: 1.0
7, 12 -- X-Sender: (Unverified)
7, 12 -- Message-Id: {p06020404c01bf993fee5-at-[203.98.91.208]}
7, 12 -- Date: Sat, 18 Feb 2006 09:07:35 +1100
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: leis-at-biochem.mpg.de (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: microtome section thickness
7, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: milton.charlton-at-utoronto.ca
Date: Fri, 17 Feb 2006 16:08:13 -0600
Subject: [Microscopy] viaWWW: NTA-nanogold for His tagged protein in EM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both milton.charlton-at-utoronto.ca as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: milton.charlton-at-utoronto.ca
Name: Milton Charlton

Organization: University of Toronto

Title-Subject: [Filtered] NTA-nanogold for His tagged protein in EM?

Question: Has anynone had success using NTA-nanogold to disclose the
location of His-tagged proteins by electron microscopy? I would like
to attach the nanogold this way before injecting the protein into a
cell. An alternative would be to apply gold labelled primary
anti-His after fixation and permeabilization.

Thanks

Milton Charlton
University of Toronto

---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Fri Feb 17 16:08:12 2006
8, 12 -- Received: from [203.98.91.208] (msdvpn25.msd.anl.gov [130.202.238.89])
8, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HM88eB023657
8, 12 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 16:08:10 -0600
8, 12 -- Mime-Version: 1.0
8, 12 -- X-Sender: (Unverified)
8, 12 -- Message-Id: {p06020405c01bf9b005d8-at-[203.98.91.208]}
8, 12 -- Date: Sat, 18 Feb 2006 09:08:06 +1100
8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: milton.charlton-at-utoronto.ca (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: NTA-nanogold for His tagged protein in EM?
8, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: johnf-at-geology.wisc.edu
Date: Fri, 17 Feb 2006 16:21:54 -0600
Subject: [Microscopy] student scholarships: NIST/MAS Particle Workshop April 2006

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Please announce and distribute to students:

4 Student Scholarships ($500) Available for support for attending and
presenting a paper/poster

PARTICLE WORKSHOP 2006

A Joint NIST / Microbeam Analysis Society
Special Topics Workshop

Dates: April 24th - 26th 2006

Location: NIST, Gaithersburg, MD

The analysis of microscopic particles represents a measurement
challenge in a diverse range of scientific, technological and
environmental disciplines. Examples of areas of interest include
nanoscale particles as building blocks for nanotechnology,
contamination in high technology manufacturing, trace forensic
detection for homeland security, and environmental sampling and
monitoring. Characterization of particle specimens is complicated by
mass, volume and morphological effects that cause well established
bulk techniques to break down. In addition, while the measurement
types under consideration at this workshop are performed on a
particle-by-particle basis, the properties of interest are often
statistical measures of populations of similar particles.

This workshop will focus on the challenges that face industrial and
governmental application of microscopic particle measurement
techniques. The practical tone of the meeting will set by speakers
representing various industries and governmental organizations who
will provide insight into their particle measurement requirements.
These speakers will be followed by sessions that focus on sample
preparation and statistical experimental design and established &
emerging measurement techniques. Instrumentation to be discussed
include SEM/EDS, AEM, TOF/SIMS, optical, FIB and scanned probe.
Each of these sessions will be concluded by a round table discussion
in which attendees are encouraged to contribute.

Registration Deadline: March 31, 2006

Students interested in applying for one of the 4 student scholarships
should immediately contact

Nicholas W. M. Ritchie
NIST Microanalysis Research Group
100 Bureau Drive STOP 8371
Gaithersburg, MD 20899-8371
301-975-3929
nicholas.ritchie-at-nist.gov


There is no registration fee: the workshop is free to all attendees.
However, space is limited and pre-registration is required by NIST
Security for entry onto the NIST campus. This rule is strictly
enforced.

Full information at
www.cstl.nist.gov/div837/Division/meetings/particleworkshop/particle.htm

==============================Original Headers==============================
9, 24 -- From johnf-at-geology.wisc.edu Fri Feb 17 16:21:54 2006
9, 24 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14])
9, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HMLs1o013872
9, 24 -- for {Microscopy-at-Microscopy.Com} ; Fri, 17 Feb 2006 16:21:54 -0600
9, 24 -- Received: from localhost (localhost [127.0.0.1])
9, 24 -- by localhost (Postfix) with ESMTP id F0BEE20D01
9, 24 -- for {Microscopy-at-Microscopy.Com} ; Fri, 17 Feb 2006 16:21:53 -0600 (CST)
9, 24 -- Received: from ice.geology.wisc.edu ([127.0.0.1])
9, 24 -- by localhost (ice [127.0.0.1]) (amavisd-new, port 10024) with ESMTP
9, 24 -- id 07362-03 for {Microscopy-at-Microscopy.Com} ;
9, 24 -- Fri, 17 Feb 2006 16:21:49 -0600 (CST)
9, 24 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57])
9, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits))
9, 24 -- (No client certificate requested)
9, 24 -- by ice.geology.wisc.edu (Postfix) with ESMTP id 9E1EA20D16
9, 24 -- for {Microscopy-at-Microscopy.Com} ; Fri, 17 Feb 2006 16:21:49 -0600 (CST)
9, 24 -- Mime-Version: 1.0
9, 24 -- Message-Id: {p0623091dc01bfc64a0eb-at-[144.92.206.57]}
9, 24 -- Date: Fri, 17 Feb 2006 16:19:32 -0600
9, 24 -- To: Microscopy-at-Microscopy.Com
9, 24 -- From: John Fournelle {johnf-at-geology.wisc.edu}
9, 24 -- Subject: student scholarships: NIST/MAS Particle Workshop April 2006
9, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
9, 24 -- X-Virus-Scanned: by amavisd-new at geology.wisc.edu
==============================End of - Headers==============================




From: bfoster-at-mme1.com
Date: Fri, 17 Feb 2006 16:31:50 -0600
Subject: [Microscopy] Re: Floaters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Bob

It has to do with the physics of simple pinhole optics. Essentially,
when you are just in the right focal plane, you are doing an "entopic
exam" of your eye. You can also reproduce the experiment by putting
a small hole (about 1/8") into a piece of cardboard (1/2 of a file
folder) and staring through it at a neutral surface (blank wall; sky,
if not too bright). Adjust the distance between the card and your
eye and, at the right distance, you will see the internal structure.

I've had an interesting array of tiny cataracts for over 25 years and
keep track of their position and size using this method.

Hope this is helpful.

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text for the Spring
semester? Call us today to learn more about "Optimizing LIght
Microscopy". Copies still available through MME... even for
class-room lots ... and we give quantity discounts. Call Ken Piel at
(972)954-8011.



At 04:05 PM 2/17/2006, bagnell-at-med.unc.edu wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
15, 17 -- From bfoster-at-mme1.com Fri Feb 17 16:31:50 2006
15, 17 -- Received: from 5starpro.com (enterprise.5starpro.com [207.44.136.95])
15, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HMVnJe023336
15, 17 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 16:31:49 -0600
15, 17 -- Received: (qmail 19789 invoked from network); 17 Feb 2006 17:33:30 -0500
15, 17 -- Received: from host57-99.rancor.birch.net (HELO barbsd505.mme1.com) (65.17.57.99)
15, 17 -- by enterprise.5starpro.com with SMTP; 17 Feb 2006 17:33:30 -0500
15, 17 -- Message-Id: {7.0.1.0.0.20060217162833.01fc9ec0-at-mme1.com}
15, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0
15, 17 -- Date: Fri, 17 Feb 2006 16:31:54 -0600
15, 17 -- To: bagnell-at-med.unc.edu, Microscopy Listserver {microscopy-at-microscopy.com}
15, 17 -- From: Barbara Foster {bfoster-at-mme1.com}
15, 17 -- Subject: Re: [Microscopy] Floaters
15, 17 -- In-Reply-To: {200602172205.k1HM5BbH017110-at-ns.microscopy.com}
15, 17 -- References: {200602172205.k1HM5BbH017110-at-ns.microscopy.com}
15, 17 -- Mime-Version: 1.0
15, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Fri, 17 Feb 2006 16:58:51 -0600
Subject: [Microscopy] Microscope cooling lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I had the same sludge problem about 1.5 years ago in
a new Haskris chiller. Haskris recommends using only
distilled water. That lasted about three months and
the slime appeared. It was a combination of algae and
small particles. Dumped the water and replaced with
new distilled water and Skasol to flush. Then, new
distilled water and one half liter of Hexid A4 from
Applied Thermal Control Ltd. UK as supplied by SEM
service tech. Chiller seized after about three months.
Post mortem indicated that the impeller blades failed.
Most likely due to misalignment of motor and pump.

Haskris replaced motor and pump assembly. Fluid was
drained and replaced with distilled water and ethylene
glycol (.5G to 4.5G distilled water). Filters were
changed and no problems for about eight months. Liquid
is not starting to become darker and small build up of
stuff in chiller main filter. It is time to change liquid and
filters. Main filter is in the water tank and is spec'd
at about 50u. External toilet paper style filter is spec'd
at 2u. So both get changed at the same time.

The Haskris unit specifically says to not use automotive
antifreeze since it will deteriorate the BUNA N material in
the chiller. Some anti freeze contains ethylene glycol.
So I'm puzzled by the successful use of distilled water and
EG. Perhaps they meant to say not to use 100% antifreeze
rather than a diluted mix.

The other factor is that the SEM came with basically transparent
water hoses. This is not good since the light gets into them
and advances the algae. So this upcoming liquid and filter
replacement will include replacing the hoses with opaque ones.

Overall, there are three aspects to be concerned about:

1. chiller guts and pump
2. hoses
3. SEM items that get chilled water (TMP, coils, etc.)

I don't think that there is a single simple answer to this
problem since SEMs are different and chillers are different.

gary g.



At 02:04 PM 2/17/2006, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
15, 20 -- From gary-at-gaugler.com Fri Feb 17 16:58:51 2006
15, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
15, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1HMwoD3002918
15, 20 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 16:58:51 -0600
15, 20 -- Received: (qmail 24885 invoked from network); 17 Feb 2006 14:58:50 -0800
15, 20 -- Received: by simscan 1.1.0 ppid: 24881, pid: 24883, t: 0.0890s
15, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
15, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211)
15, 20 -- by qsmtp3 with SMTP; 17 Feb 2006 14:58:50 -0800
15, 20 -- Message-Id: {6.2.3.4.2.20060217143542.0205ed40-at-mail.calweb.com}
15, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4
15, 20 -- Date: Fri, 17 Feb 2006 14:58:51 -0800
15, 20 -- To: jfactor-at-ns.purchase.edu
15, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
15, 20 -- Subject: Re: [Microscopy] Re: Microscope cooling lines
15, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
15, 20 -- In-Reply-To: {200602172204.k1HM4d7s015164-at-ns.microscopy.com}
15, 20 -- References: {200602172204.k1HM4d7s015164-at-ns.microscopy.com}
15, 20 -- Mime-Version: 1.0
15, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: dljones-at-bestweb.net
Date: Fri, 17 Feb 2006 20:43:14 -0600
Subject: [Microscopy] Re: Microscope cooling lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Gary,

Just a couple of points I'd like to make w.r.t. your post.

I would not recommend using ethylene glycol as it is toxic. Propylene
glycol is non-toxic.

The problem with using "antifreeze" mixtures not intended for use in these
kinds of systems is that they do not usually contain the proper
additives. Glycol solutions that are not HVAC grade will deteriorate over
time through a type of polymerization that will plug things up and render
the system inoperable. The resulting deposits thus formed are very inert
and to my knowledge no one has ever found a way to clean them so you
basically have to replace the chiller system.

HVAC grade polypropylene contains additives to avoid that problem plus
inhibitors that stop corrosion of most commercially available materials in
piping. That also includes seal materials, but I'm not sure about
specifically N-buena seals. I'd have to look that up, but I would think it
also compatible being such a commonly used seal material.

Biofouling is quite common in closed loop systems. Using a biocide is
usually used in these systems to eliminate this problem.

The original poster to this thread likely is in a location where they have
a professional water treatment company taking care of large HVAC systems.
Perhaps they should talk with the representative of that company and find
out what is being done for chemical treatment of chilled water systems
there. They may be able to just get some of the proper chemicals that
likely exist on-site already.

I would also like to point out, there is really no reason to go through
the expense of using a glycol based system unless there is danger of
freezing the coolant for some reason. There are numerous other water
treatments that are much less expensive and work very well to keep a
closed loop system running well. If there is little to no make up water
needed for the closed system, once set-up properly, there is little more
to do other than enjoy a clean running system...

dj

On Fri, 17 Feb 2006, gary-at-gaugler.com wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I had the same sludge problem about 1.5 years ago in
} a new Haskris chiller. Haskris recommends using only
} distilled water. That lasted about three months and
} the slime appeared. It was a combination of algae and
} small particles. Dumped the water and replaced with
} new distilled water and Skasol to flush. Then, new
} distilled water and one half liter of Hexid A4 from
} Applied Thermal Control Ltd. UK as supplied by SEM
} service tech. Chiller seized after about three months.
} Post mortem indicated that the impeller blades failed.
} Most likely due to misalignment of motor and pump.
}
} Haskris replaced motor and pump assembly. Fluid was
} drained and replaced with distilled water and ethylene
} glycol (.5G to 4.5G distilled water). Filters were
} changed and no problems for about eight months. Liquid
} is not starting to become darker and small build up of
} stuff in chiller main filter. It is time to change liquid and
} filters. Main filter is in the water tank and is spec'd
} at about 50u. External toilet paper style filter is spec'd
} at 2u. So both get changed at the same time.
}
} The Haskris unit specifically says to not use automotive
} antifreeze since it will deteriorate the BUNA N material in
} the chiller. Some anti freeze contains ethylene glycol.
} So I'm puzzled by the successful use of distilled water and
} EG. Perhaps they meant to say not to use 100% antifreeze
} rather than a diluted mix.
}
} The other factor is that the SEM came with basically transparent
} water hoses. This is not good since the light gets into them
} and advances the algae. So this upcoming liquid and filter
} replacement will include replacing the hoses with opaque ones.
}
} Overall, there are three aspects to be concerned about:
}
} 1. chiller guts and pump
} 2. hoses
} 3. SEM items that get chilled water (TMP, coils, etc.)
}
} I don't think that there is a single simple answer to this
} problem since SEMs are different and chillers are different.
}
} gary g.
}
}
}
} At 02:04 PM 2/17/2006, you wrote:
}
}
}
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } My service engineer recommends neither tap nor distilled water, but
} } rather bottled spring water. Has anyone yet mentioned the possibility
} } that green sludge in the filter might be algae growing in the cooling
} } water or the cooling unit?
} } --Jan Factor
} }
} } ---------------------------------------
} } Jan Robert Factor, Ph.D.
} } Professor of Biology
} } ---------------------------------------
} } Natural Sciences
} } Purchase College, State University of New York
} } 735 Anderson Hill Rd.
} } Purchase, NY 10577
} } USA
} } ---------------------------------------
} } Office Tel: 914-251-6659
} } Office Fax: 914-251-6635
} } E-mail: jfactor-at-ns.purchase.edu
} } or- jan.factor-at-purchase.edu
} } ---------------------------------------
} }
} }
} }
} } ==============================Original Headers==============================
} } 4, 16 -- From jfactor-at-ns.purchase.edu Fri Feb 17 16:02:30 2006
} } 4, 16 -- Received: from zephyr.ns.purchase.edu
} } (zephyr.ns.purchase.edu [199.79.168.193])
} } 4, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
} } k1HM2PAx009529
} } 4, 16 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006
} } 16:02:25 -0600
} } 4, 16 -- Received: from [10.52.0.79] ([10.52.0.79])
} } 4, 16 -- by zephyr.ns.purchase.edu (AIX5.1/8.11.6p2/8.11.0)
} } with ESMTP id k1HM5lr17594;
} } 4, 16 -- Fri, 17 Feb 2006 17:05:48 -0500
} } 4, 16 -- Message-ID: {43F647F0.1070409-at-ns.purchase.edu}
} } 4, 16 -- Date: Fri, 17 Feb 2006 17:02:24 -0500
} } 4, 16 -- From: Jan Factor {jfactor-at-ns.purchase.edu}
} } 4, 16 -- User-Agent: Thunderbird 1.5 (Windows/20051201)
} } 4, 16 -- MIME-Version: 1.0
} } 4, 16 -- To: microscopy-at-microscopy.com
} } 4, 16 -- Subject: Re: Microscope cooling lines
} } 4, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} } 4, 16 -- Content-Transfer-Encoding: 7bit
} } ==============================End of - Headers==============================
}
}
} ==============================Original Headers==============================
} 15, 20 -- From gary-at-gaugler.com Fri Feb 17 16:58:51 2006
} 15, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
} 15, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1HMwoD3002918
} 15, 20 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 16:58:51 -0600
} 15, 20 -- Received: (qmail 24885 invoked from network); 17 Feb 2006 14:58:50 -0800
} 15, 20 -- Received: by simscan 1.1.0 ppid: 24881, pid: 24883, t: 0.0890s
} 15, 20 -- scanners: regex: 1.1.0 attach: 1.1.0
} 15, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211)
} 15, 20 -- by qsmtp3 with SMTP; 17 Feb 2006 14:58:50 -0800
} 15, 20 -- Message-Id: {6.2.3.4.2.20060217143542.0205ed40-at-mail.calweb.com}
} 15, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4
} 15, 20 -- Date: Fri, 17 Feb 2006 14:58:51 -0800
} 15, 20 -- To: jfactor-at-ns.purchase.edu
} 15, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
} 15, 20 -- Subject: Re: [Microscopy] Re: Microscope cooling lines
} 15, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com}
} 15, 20 -- In-Reply-To: {200602172204.k1HM4d7s015164-at-ns.microscopy.com}
} 15, 20 -- References: {200602172204.k1HM4d7s015164-at-ns.microscopy.com}
} 15, 20 -- Mime-Version: 1.0
} 15, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
} ==============================End of - Headers==============================
}


==============================Original Headers==============================
12, 21 -- From "dljones-at-bestweb.net"-at-bestweb.net Fri Feb 17 20:43:13 2006
12, 21 -- Received: from mta4.srv.hcvlny.cv.net (mta4.srv.hcvlny.cv.net [167.206.4.199])
12, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1I2hDlf008914
12, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 20:43:13 -0600
12, 21 -- Received: from localhost (ool-44c62883.dyn.optonline.net [68.198.40.131])
12, 21 -- by mta4.srv.hcvlny.cv.net
12, 21 -- (Sun Java System Messaging Server 6.2-4.03 (built Sep 22 2005))
12, 21 -- with ESMTP id {0IUV00D2N3JYUD00-at-mta4.srv.hcvlny.cv.net} for
12, 21 -- microscopy-at-microscopy.com; Fri, 17 Feb 2006 21:43:13 -0500 (EST)
12, 21 -- Date: Fri, 17 Feb 2006 21:43:12 -0500 (Eastern Standard Time)
12, 21 -- From: "David L. Jones" {"dljones-at-bestweb.net"-at-bestweb.net}
12, 21 -- Subject: Re: [Microscopy] Microscope cooling lines
12, 21 -- In-reply-to: {200602172305.k1HN5bhk012207-at-ns.microscopy.com}
12, 21 -- X-X-Sender: dljones-at-pop-croton.bestweb.net
12, 21 -- To: gary-at-gaugler.com
12, 21 -- Cc: microscopy-at-microscopy.com
12, 21 -- Message-id: {Pine.WNT.4.64.0602172109370.408-at-dljtoshiba}
12, 21 -- MIME-version: 1.0
12, 21 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed
12, 21 -- Content-transfer-encoding: 7BIT
12, 21 -- References: {200602172305.k1HN5bhk012207-at-ns.microscopy.com}
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Fri, 17 Feb 2006 20:51:47 -0600
Subject: [Microscopy] Re: Microscope cooling lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for the input.

If EG is toxic and PG is not, that is a factor. However,
I do not care if one or the other is toxic. I do not swim
in the 5 gallons of fluid. So, between the two glycols,
which is best for SEM? I do not know. What do you think?

So there are toxic issues, corrosive issues, etc., etc.
Well, then just dump the SEM, eh? No. I do not think so.
One must work with the materials at hand. So, what do you
think are the best materials for chiller fluid?

gary g.



At 06:43 PM 2/17/2006, you wrote:
} Gary,
}
} Just a couple of points I'd like to make w.r.t. your post.
}
} I would not recommend using ethylene glycol as it is toxic.
} Propylene glycol is non-toxic.
}
} The problem with using "antifreeze" mixtures not intended for use in
} these kinds of systems is that they do not usually contain the
} proper additives. Glycol solutions that are not HVAC grade will
} deteriorate over time through a type of polymerization that will
} plug things up and render the system inoperable. The resulting
} deposits thus formed are very inert and to my knowledge no one has
} ever found a way to clean them so you basically have to replace the
} chiller system.
}
} HVAC grade polypropylene contains additives to avoid that problem
} plus inhibitors that stop corrosion of most commercially available
} materials in piping. That also includes seal materials, but I'm not
} sure about specifically N-buena seals. I'd have to look that up, but
} I would think it also compatible being such a commonly used seal material.
}
} Biofouling is quite common in closed loop systems. Using a biocide
} is usually used in these systems to eliminate this problem.
}
} The original poster to this thread likely is in a location where
} they have a professional water treatment company taking care of
} large HVAC systems. Perhaps they should talk with the representative
} of that company and find out what is being done for chemical
} treatment of chilled water systems there. They may be able to just
} get some of the proper chemicals that likely exist on-site already.
}
} I would also like to point out, there is really no reason to go
} through the expense of using a glycol based system unless there is
} danger of freezing the coolant for some reason. There are numerous
} other water treatments that are much less expensive and work very
} well to keep a closed loop system running well. If there is little
} to no make up water needed for the closed system, once set-up
} properly, there is little more to do other than enjoy a clean running system...
}
} dj
}
} On Fri, 17 Feb 2006, gary-at-gaugler.com wrote:
}
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 21 -- From gary-at-gaugler.com Fri Feb 17 20:51:47 2006
9, 21 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
9, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1I2pkQd018305
9, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 20:51:47 -0600
9, 21 -- Received: (qmail 22693 invoked from network); 17 Feb 2006 18:51:46 -0800
9, 21 -- Received: by simscan 1.1.0 ppid: 22690, pid: 22691, t: 0.2511s
9, 21 -- scanners: regex: 1.1.0 attach: 1.1.0
9, 21 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211)
9, 21 -- by qsmtp3 with SMTP; 17 Feb 2006 18:51:46 -0800
9, 21 -- Message-Id: {6.2.3.4.2.20060217184632.02068418-at-mail.calweb.com}
9, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4
9, 21 -- Date: Fri, 17 Feb 2006 18:51:46 -0800
9, 21 -- To: "David L. Jones" {"dljones-at-bestweb.net"-at-bestweb.net}
9, 21 -- From: Gary Gaugler {gary-at-gaugler.com}
9, 21 -- Subject: Re: [Microscopy] Microscope cooling lines
9, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com}
9, 21 -- In-Reply-To: {Pine.WNT.4.64.0602172109370.408-at-dljtoshiba}
9, 21 -- References: {200602172305.k1HN5bhk012207-at-ns.microscopy.com}
9, 21 -- {Pine.WNT.4.64.0602172109370.408-at-dljtoshiba}
9, 21 -- Mime-Version: 1.0
9, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: tonygr-at-MIT.EDU
Date: Sat, 18 Feb 2006 07:54:12 -0600
Subject: [Microscopy] TEM film electrostatic discharging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This is a perennial problem, I know, but does anyone have any effective "pet" solutions to the problem of charging and subsequent electrostatic arcing of TEM film in the extremely dry air we get in New England typically in the winter, and some other people get at other seasons of the year?

The discharges can occur at almost any stage of the handling, from getting the film out of the vendor's packing, loading and unloading in the microscope carriers and loading in the developing racks (we use Lucite ones). We have considered a humidifier in the darkroom, but that would involve blocking off the ventilation (we have make-up air positively blown into to room and exhaust actively sucked out) to prevent our moisture from being simply blown away.

Any hints would be welcome, but switching to digital imaging is one that is not going to happen.

Tony.

*************************************
Anthony J. Garratt-Reed M.A. D.Phil.
MIT Room #13-1027
77 Massachusetts Avenue
Cambridge, Massachusetts 02139-4307
USA

Tel: (617) 253-4622
Fax: (617) 258-6478
*************************************


==============================Original Headers==============================
7, 26 -- From tonygr-at-MIT.EDU Sat Feb 18 07:54:11 2006
7, 26 -- Received: from biscayne-one-station.mit.edu (BISCAYNE-ONE-STATION.MIT.EDU [18.7.7.80])
7, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1IDsB7m003310
7, 26 -- for {microscopy-at-microscopy.com} ; Sat, 18 Feb 2006 07:54:11 -0600
7, 26 -- Received: from outgoing.mit.edu (OUTGOING-AUTH.MIT.EDU [18.7.22.103])
7, 26 -- by biscayne-one-station.mit.edu (8.12.4/8.9.2) with ESMTP id k1IDp3gq004012
7, 26 -- for {microscopy-at-microscopy.com} ; Sat, 18 Feb 2006 08:51:03 -0500 (EST)
7, 26 -- Received: from [192.168.1.47] (pool-72-70-109-28.bstnma.east.verizon.net [72.70.109.28])
7, 26 -- (authenticated bits=0)
7, 26 -- (User authenticated as tonygr-at-ATHENA.MIT.EDU)
7, 26 -- by outgoing.mit.edu (8.13.1/8.12.4) with ESMTP id k1IDosBT005294
7, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
7, 26 -- for {microscopy-at-microscopy.com} ; Sat, 18 Feb 2006 08:51:01 -0500 (EST)
7, 26 -- Message-ID: {43F7263D.9010808-at-mit.edu}
7, 26 -- Date: Sat, 18 Feb 2006 08:50:53 -0500
7, 26 -- From: Tony Garratt-Reed {tonygr-at-MIT.EDU}
7, 26 -- User-Agent: Thunderbird 1.5 (Windows/20051201)
7, 26 -- MIME-Version: 1.0
7, 26 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com}
7, 26 -- Subject: TEM film electrostatic discharging
7, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
7, 26 -- Content-Transfer-Encoding: 7bit
7, 26 -- X-Spam-Score: 1.217
7, 26 -- X-Spam-Level: * (1.217)
7, 26 -- X-Spam-Flag: NO
7, 26 -- X-Scanned-By: MIMEDefang 2.42
==============================End of - Headers==============================




From: dsherman-at-purdue.edu
Date: Sat, 18 Feb 2006 10:40:08 -0600
Subject: [Microscopy] Cooling lines-responses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

Below are responses (abbreviated) to my question about which to use, copper
or PVC, to replace water lines running from cooling units to microscopes. I
will run any suggestions by my service engineers just to make sure there are
no concerns regarding specific equipment. I do know that water pH is
important and that may play a role in determining what additives to use:


I am a service engineer who works on electron microscopes for the last
17 years and my point of view: I would go with copper this way no matter
what may or may not happen to the lines (cleaning out - whatever) you
have no worries. (Ray Spengler)

I do not think that there will be a problem using copper or PVC. But you
chould check with the eq maker to see if they have any issues.
We use Copper and have not had any problems. We do get build up over time
due to the hoses "rotting" over time and general scale buildup. (Karl Weiss)

USE PVC and make the final connection to the equipment with two coils of
rubber hose for vibration control. ( Fran Laabs)

For our new IMAGE building, I specified PVC piping for the closed
circ loop between the water chillers and scopes. We still notice a
greenish sludge building up in the water filters (takes about 6-8
months to become significant) but I am certain that this is coming
from the EM (copper cooling coils and iron
connections---} electrolytic reaction). The EM service people told us
that if we ever used acid to clean the lines that they would no
longer warranty the microscope. The PVC lines are perfectly clean. (J.
Bozzola)

Another material you may wish to consider is called PEX, which is a
cross-linked polyethylene. I don't know too much about its characteristics,
except that it's very smooth inside, which should retard crud accumulation
and it's more opaque than white pvc. It may be worth checking out. (P.
Grover)

As an FYI, if you have copper based and iron based materials mixed in the
same system, the iron will corrode preferentially through galvanic
coupling, not the copper. In fact, the iron becomes a sacrificial anode
protecting the
copper from corrosion. Any time you have those two materials in the same
system, you should have dielectric couplings between the two or you will
actively corrode the ferrous based material.
Regarding acid cleaning, you should know what your deposits are in your
piping before deciding how to clean them. As an FYI, copper will generally
corrode at pH's below about 6.3 or so. There are low pH cleaners that can be
used with copper, but they contain corrosion inhibitors. (D. Jones)

If you intend to clean the lines with acid, I suggest PVC, since Cu
can be etched at low pH. In addition to floating some dichlorophene
for algal control, we add a corrosion inhibitor. We have been using a
Mo-based formula, which was available from Aqua Labs on the East coast
and from Skasol on the West coast, so find a distributor in your area.
I think that either Aqua or Skasol would be able to give you that info.(B.
Tivol)

Along these lines, I noticed a lot more copper leaching into the
lines when I used deionized water than regular or filtered tap water.
Secondly, Why hasn't anyone suggested using antifreeze? Antifreeze
intended for cars should have corrosion inhibitors and should be
suitable for this purpose.
I have still gotten green particles regardless of whether antifreeze
was used or not and have regularly made it a practice to clean the
filters in the lines once a semester. (Jerry Calvin)

I have used the ethylene/glycol/water mix through
clear PVC piping between my circulator and TEM and
vacuum coating unit for six years now. There is a long
run of the piping in daylight. Absolutely no algae
Problem. Use 50/50 ethylene glycol*/water and PVC pipes and you
will never have a problem again.
Make sure that the chiller/recirculator manufacturer
OKs the use of ethylene glycol but I wouldn't
anticipate any problem. Even 20/80 EG/Water will work
well. (Ted Dunn)


Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy



==============================Original Headers==============================
17, 21 -- From dsherman-at-purdue.edu Sat Feb 18 10:40:08 2006
17, 21 -- Received: from exchange.purdue.edu (1061exfe02.adpc.purdue.edu [128.210.63.223])
17, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1IGe8dl014778
17, 21 -- for {microscopy-at-microscopy.com} ; Sat, 18 Feb 2006 10:40:08 -0600
17, 21 -- Received: from EXCH02.purdue.lcl ([128.210.63.234]) by exchange.purdue.edu with Microsoft SMTPSVC(6.0.3790.211);
17, 21 -- Sat, 18 Feb 2006 11:40:08 -0500
17, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH02.purdue.lcl ([128.210.63.237]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.239]) with Microsoft Exchange Server HTTP-DAV ;
17, 21 -- Sat, 18 Feb 2006 16:40:08 +0000
17, 21 -- User-Agent: Microsoft-Entourage/11.2.1.051004
17, 21 -- Date: Sat, 18 Feb 2006 11:40:07 -0500
17, 21 -- Subject: Cooling lines-responses
17, 21 -- From: Debby Sherman {dsherman-at-purdue.edu}
17, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
17, 21 -- Message-ID: {C01CB817.D64C%dsherman-at-purdue.edu}
17, 21 -- Thread-Topic: Cooling lines-responses
17, 21 -- Thread-Index: AcY0qfmyOEGiJKCdEdqwBAARJN08Mg==
17, 21 -- Mime-version: 1.0
17, 21 -- Content-type: text/plain;
17, 21 -- charset="US-ASCII"
17, 21 -- Content-transfer-encoding: 7bit
17, 21 -- X-OriginalArrivalTime: 18 Feb 2006 16:40:08.0197 (UTC) FILETIME=[FA697350:01C634A9]
==============================End of - Headers==============================




From: soleimanij-at-tbzmed.ac.ir
Date: Sat, 18 Feb 2006 16:31:57 -0600
Subject: [Microscopy] viaWWW: scale bar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both soleimanij-at-tbzmed.ac.ir as well as the
MIcroscopy Listserver
---------------------------------------------------------------------------

Email: soleimanij-at-tbzmed.ac.ir
Name: Jafar Soleimani Rad

Organization: University

Title-Subject: [Filtered] scale bar

Question: Hi
we are using A LEO 906 TEM, scale bar is not registered in the
microfilms. We are having problem to calculating the real sizes. any
help in this matter is appreciated.

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Sat Feb 18 16:31:56 2006
6, 12 -- Received: from [203.98.91.159] (msdvpn21.msd.anl.gov [130.202.238.85])
6, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1IMVpRQ027751
6, 12 -- for {microscopy-at-microscopy.com} ; Sat, 18 Feb 2006 16:31:54 -0600
6, 12 -- Mime-Version: 1.0
6, 12 -- X-Sender: (Unverified)
6, 12 -- Message-Id: {p06020402c01d50ba395b-at-[203.98.91.159]}
6, 12 -- Date: Sun, 19 Feb 2006 09:31:50 +1100
6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: soleimanij-at-tbzmed.ac.ir (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: scale bar
6, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: rpowell-at-nanoprobes.com
Date: Sat, 18 Feb 2006 16:32:54 -0600
Subject: [Microscopy] viaWWW: NTA-nanogold for His tagged protein in EM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rpowell-at-nanoprobes.com as well
as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rpowell-at-nanoprobes.com
Name: Rick Powell

Organization: Nanoprobes, Incorporated

Title-Subject: [Filtered] Re: [Microscopy]:
NTA-nanogold for His tagged protein in EM?

Question: [Commercial disclaimer - I work for
Nanoprobes, and we make NTA-Ni(II)-NanogoldÆ]

Hello Milton:

Here are a few recent references for EM using
NTA-Ni(II)-Nanogold, with links to articles on
our online newsletter that describe them:

(1) Wolfe, C. L.; Warrington, J. A.; Treadwell,
L., and Norcum, M. T.: A three-dimensional
working model of the multienzyme complex of
aminoacyl-tRNA synthetases based on electron
microscopic placements of tRNA and proteins. J.
Biol. Chem., 280, 38870-38878 (2005).

Newsletter article:
http://www.nanoprobes.com/Vol6_Iss12.html#3

(2) Bumba, L.; Tichy, M.; Dobakova, M.; Komenda,
J., and Vacha, F.: Localization of the PsbH
subunit in photosystem II from the Synechocystis
6803 using the His-tagged NiñNTA Nanogold
labeling. J. Struct. Biol., 152, 28-35 (2005).

Newsletter article:
http://www.nanoprobes.com/Vol6_Iss10.html#1

There are two more recent references in which the
reagent was used to label polyhistidine-tagged
components of viral capsids:

(3) Chatterji, A.; Ochoa, W. F.; Ueno, T.; Lin
T., and Johnson, J. E.: A virus-based nanoblock
with tunable electrostatic properties. Nano
Lett., 5, 597-602 (2005).

Newsletter article:
http://www.nanoprobes.com/Vol6_Iss5.html#1

(4) Collins, R. F.; Frye, S. A.; Balasingham, S.;
Ford, R. C.; Tonjum, T., and Derrick, J. P.:
Interaction with type IV pili induces structural
changes in the bacterial outer membrane secretin
PilQ. J. Biol. Chem., 280, 18923-18930 (2005).

Newsletter article:
http://www.nanoprobes.com/Vol6_Iss6.html#6

Details about the reagent are available on our web site:

Catalog and general info:
http://www.nanoprobes.com/NTAgold.html

Product info and instructions:
http://www.nanoprobes.com/Inf2080.html

We have found that the complexes of
NTA-Ni(II)-Nanogold bound to polyhis-tagged
proteins hold together well during chromtographic
separations (gel filtration), implying that they
should be injectable.

Hope some of these are helpful,

Rick Powell
Nanoprobes, Inc.
www.nanoprobes.com


At 05:09 PM 2/17/2006, you wrote:
Question: Has anynone had success using NTA-nanogold to disclose the
location of His-tagged proteins by electron microscopy? I would like
to attach the nanogold this way before injecting the protein into a
cell. An alternative would be to apply gold labelled primary
anti-His after fixation and permeabilization.

Thanks

Milton Charlton
University of Toronto

---------------------------------------------------------------------------


---------------------------------------------------------------------------


==============================Original Headers==============================
30, 14 -- From zaluzec-at-microscopy.com Sat Feb 18 16:32:54 2006
30, 14 -- Received: from [203.98.91.159] (msdvpn21.msd.anl.gov [130.202.238.85])
30, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1IMWnGA028544
30, 14 -- for {microscopy-at-microscopy.com} ; Sat, 18 Feb 2006 16:32:51 -0600
30, 14 -- Mime-Version: 1.0
30, 14 -- X-Sender: (Unverified)
30, 14 -- Message-Id: {p06020403c01d50e242aa-at-[203.98.91.159]}
30, 14 -- Date: Sun, 19 Feb 2006 09:32:47 +1100
30, 14 -- To: microscopy-at-microscopy.com
30, 14 -- From: rpowell-at-nanoprobes.com (by way of MicroscopyListserver)
30, 14 -- Subject: viaWWW: NTA-nanogold for His tagged protein in EM?
30, 14 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
30, 14 -- Content-Transfer-Encoding: 8bit
30, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1IMWnGA028544
==============================End of - Headers==============================




From: grmitch-at-netzero.com
Date: Sun, 19 Feb 2006 20:30:43 -0600
Subject: [Microscopy] AskAMicroscopist: teaching a unit on microscopic life to 5th

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question was submitted to Ask-A-Microscopist by (grmitch-at-netzero.com)
from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, February 15, 2006 at 20:08:01
Remember to consider the Grade/Age of the student when considering the Question
---------------------------------------------------------------------------
Please reply to both grmitch-at-netzero.com as well as to the Microscopy Listserver
---------------------------------------------------------------------------

Email: grmitch-at-netzero.com
Name: Linda Mitchell

Organization: BPS Environmental Center

Education: K-8 Grade Grammar School

Location: Birmingham, Michigan USA

Title: Microorganisms!

Question: I will be teaching a unit on microscopic life to 5th graders next month, March (quite a cold month here in Michigan). My question for you:
What is the best way to keep my microorganisms alive throughout the program? We obtain our samples directly from the 10 acres of the property here at the nature center. One of the samples is from our pond area and the second from the swamp woods area. We have no difficulty in finding a healthy sample, yet the problem that we often encounter is keeping them alive. We have supplies - lab pans, microscopes, even an aquarium that is our "indoor pond" when the water ices over. Do you have any feeding, climate tips that would help in these areas? This is a program that is enjoyed by both the staff and students - they are so amazed by what they find. Thanks for your input.

---------------------------------------------------------------------------

==============================Original Headers==============================
8, 13 -- From zaluzec-at-ultra5.microscopy.com Sun Feb 19 20:30:43 2006
8, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1K2Uei6000661
8, 13 -- for {microscopy-at-microscopy.com} ; Sun, 19 Feb 2006 20:30:42 -0600
8, 13 -- Mime-Version: 1.0
8, 13 -- X-Sender: zaluzec-at-ultra5.microscopy.com (Unverified)
8, 13 -- Message-Id: {p06110400c01eda3d4eac-at-[206.69.208.22]}
8, 13 -- Date: Sun, 19 Feb 2006 20:30:39 -0600
8, 13 -- To: microscopy-at-microscopy.com
8, 13 -- From: grmitch-at-netzero.com (by way of Ask-A-Microscopist)
8, 13 -- Subject: AskAMicroscopist: teaching a unit on microscopic life to 5th
8, 13 -- graders
8, 13 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: Laurine.Ottmar-at-millenniumchem.com
Date: Mon, 20 Feb 2006 06:02:31 -0600
Subject: [Microscopy] Employment Opportunity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Microscopist

Millennium Chemicals (a Lyondell Company), the world's second largest
producer of Titanium Dioxide, is seeking a microscopist with experience in
the areas of Scanning Electron Microscopy (SEM), Transmission Electron
Microscopy (TEM), Atomic Force Microscopy (AFM) and X-ray Diffraction (XRD)
to support R&D, Commercial and Manufacturing activities at its Baltimore
Research Center.

EDUCATIONAL REQUIREMENTS

Minimum M.S. in chemistry, mineralogy, material science or similar field,
with 7-10 years experience in an industrial R&D environment preferred.

DESCRIPTION:

The primary responsibility of the position is the application of advanced
microscopic techniques to investigate properties, mineralogy, phase
distribution, morphology and structure/function relationships of pigmentary
and catalytic TiO2 particles, catalysts and polymers. In addition to
conducting research and and support activities, the candidate will be
responsible for oversight and maintenance of a state-of-the-art microscopy
laboratory that includes:

*Olympus SZX7 stereoscope
*Olympus BX51 Optical Light Microscope
*Amray 1930 SEM with EDS (scheduled for replacement in 2006)
*Jeol 2000 FX2 with EDS and STEM
*Veeco Nanoscope 3A Dimension 3100 AFM
*Panalytical X'pert Pro XRD
*RMC PT-X Ultramicrotome with CR-X Cryosectioning system
*Gatan Model 691 Precision Ion Polishing System
*SPE Plasma Prep II Plasma Etcher/Asher
*Denton sputterer/coater

SALARY

Salary is commensurate with education and experience. Millennium Chemicals
(A Lyondell Company) offers a competitive benefits package including
relocation.

For more information and to submit a resume online, please visit our
website:

http://www.millenniumchem.com/Careers/Current+Opportunities/

Millennium Chemicals (a Lyondell Company) is an Equal Opportunity Employer
M/F/D/V






Lyondell Chemical Company

The information contained in this e-mail message and any attachments may be confidential. It is intended only for the use of the individual or entities named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail at the originating address.


==============================Original Headers==============================
20, 24 -- From Laurine.Ottmar-at-millenniumchem.com Mon Feb 20 06:02:30 2006
20, 24 -- Received: from edcpap01.lyo.com (mail3.lyondell.com [161.16.0.77])
20, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1KC2Ucr017418
20, 24 -- for {microscopy-at-microscopy.com} ; Mon, 20 Feb 2006 06:02:30 -0600
20, 24 -- Received: from edcexp02.lyondell.com ([161.16.33.40])
20, 24 -- by edcpap01.lyo.com (8.13.4/8.13.4) with ESMTP id k1KC2Nb9003328
20, 24 -- for {microscopy-at-microscopy.com} ; Mon, 20 Feb 2006 06:02:23 -0600
20, 24 -- Received: from amnhhtv01.millenniumchem.com ([172.19.0.46]) by edcexp02.lyondell.com with Microsoft SMTPSVC(5.0.2195.6713);
20, 24 -- Mon, 20 Feb 2006 06:02:24 -0600
20, 24 -- Subject: Employment Opportunity
20, 24 -- To: microscopy-at-microscopy.com
20, 24 -- X-Mailer: Lotus Notes Release 5.0.11 July 24, 2002
20, 24 -- Message-ID: {OF1550363E.97FA6230-ON8525711A.006B77CF-8525711B.0042227A-at-millenniumchem.com}
20, 24 -- From: Laurine.Ottmar-at-millenniumchem.com
20, 24 -- Date: Mon, 20 Feb 2006 07:02:22 -0500
20, 24 -- X-MIMETrack: Serialize by Router on AMNHHTV01/HUB/SRV/MIC(Release 5.0.11 |July 24, 2002) at
20, 24 -- 02/20/2006 07:02:24 AM
20, 24 -- MIME-Version: 1.0
20, 24 -- Content-type: text/plain; charset=iso-8859-1
20, 24 -- X-OriginalArrivalTime: 20 Feb 2006 12:02:24.0991 (UTC) FILETIME=[83317EF0:01C63615]
20, 24 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 mlx=0 adultscore=0 adjust=0 reason=mlx engine=3.1.0-06021401 definitions=3.0.0-06022000
20, 24 -- X-Proofpoint-OriginalSender: Laurine.Ottmar-at-millenniumchem.com
20, 24 -- Content-Transfer-Encoding: 8bit
20, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1KC2Ucr017418
==============================End of - Headers==============================




From: richard.beanland-at-bookham.com
Date: Mon, 20 Feb 2006 10:38:12 -0600
Subject: [Microscopy] Re: TEM film electrostatic discharging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Tony

one thing that occurs to me is that you will probably have vinyl
flooring in your darkroom. This may be a major source of static and it
may be possible to get some advice on low static flooring. I know we
had some installed in one of our microscope cubicles and it didn't seem
to be tremendously expensive at the time.

If you think that the floor is part of the problem then it might be
worth checking whether the soles of your shoes are man made (create
static) or leather.

If you want to go for a tried and tested electronics industry route
look for ionisers (rather than de-ionisers) I did a simple Google
search on ioniser and static and came up with a few using
keywords "ionisers static"
such as http://www.buystatic.com/static_eliminators.htm?ioniser.htm

I was hoping that you could get away with a domestic ioniser - the sort
they sell to simulate sea or mountain air and supposedly gets rid of
headaches. But apparently they aren't much good at eliminating static
whereas the industrial ones are.

Good luck

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK

e-mail: malcolm.haswell-at-sunderland.ac.uk

----- Original Message -----
X-from: tonygr-at-MIT.EDU

I have a friend who seems particularly prone to this even when the
humidity is not very low - she can produce amazing patterns of sparks
and spirals on a negative simply by waving her hands over them!

The simple way of getting rid of electrostatic problems in a
semiconductor company like mine is to 'borrow' an earth strap and mat
from the test area. It solved the problem completely. I guess you may
have to buy them..

Good luck

Richard

________________________________________
Richard Beanland
Analytical Services
Bookham Inc
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________

} -------------------------------------------------------------------
} ---------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} AmericaTo Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserverOn-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html------------
} ----------------------------------------------------------------
}
} This is a perennial problem, I know, but does anyone have any
} effective "pet" solutions to the problem of charging and
} subsequent electrostatic arcing of TEM film in the extremely dry
} air we get in New England typically in the winter, and some other
} people get at other seasons of the year?
}
} The discharges can occur at almost any stage of the handling, from
} getting the film out of the vendor's packing, loading and
} unloading in the microscope carriers and loading in the developing
} racks (we use Lucite ones). We have considered a humidifier in
} the darkroom, but that would involve blocking off the ventilation
} (we have make-up air positively blown into to room and exhaust
} actively sucked out) to prevent our moisture from being simply
} blown away.
}
} Any hints would be welcome, but switching to digital imaging is
} one that is not going to happen.
}
} Tony.
}
} *************************************
} Anthony J. Garratt-Reed M.A. D.Phil.
} MIT Room #13-1027
} 77 Massachusetts Avenue
} Cambridge, Massachusetts 02139-4307
} USA
}
} Tel: (617) 253-4622
} Fax: (617) 258-6478
} *************************************
}
}
} ==============================Original
} Headers==============================7, 26 -- From tonygr-at-MIT.EDU
} Sat Feb 18 07:54:11 2006
} 7, 26 -- Received: from biscayne-one-station.mit.edu (BISCAYNE-ONE-
} STATION.MIT.EDU [18.7.7.80])
} 7, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
} k1IDsB7m0033107, 26 -- for {microscopy-at-microscopy.com} ; Sat,
18
} Feb 2006 07:54:11 -0600
} 7, 26 -- Received: from outgoing.mit.edu (OUTGOING-AUTH.MIT.EDU
} [18.7.22.103])7, 26 -- by biscayne-one-station.mit.edu
} (8.12.4/8.9.2) with ESMTP id k1IDp3gq004012
} 7, 26 -- for {microscopy-at-microscopy.com} ; Sat, 18 Feb 2006
} 08:51:03 -0500 (EST)
} 7, 26 -- Received: from [192.168.1.47] (pool-72-70-109-
} 28.bstnma.east.verizon.net [72.70.109.28])
} 7, 26 -- (authenticated bits=0)
} 7, 26 -- (User authenticated as tonygr-at-ATHENA.MIT.EDU)
} 7, 26 -- by outgoing.mit.edu (8.13.1/8.12.4) with ESMTP id
} k1IDosBT0052947, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-
AES256-
} SHA bits=256 verify=NOT)
} 7, 26 -- for {microscopy-at-microscopy.com} ; Sat, 18 Feb 2006
} 08:51:01 -0500 (EST)
} 7, 26 -- Message-ID: {43F7263D.9010808-at-mit.edu}
} 7, 26 -- Date: Sat, 18 Feb 2006 08:50:53 -0500
} 7, 26 -- From: Tony Garratt-Reed {tonygr-at-MIT.EDU}
} 7, 26 -- User-Agent: Thunderbird 1.5 (Windows/20051201)
} 7, 26 -- MIME-Version: 1.0
} 7, 26 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com}
} 7, 26 -- Subject: TEM film electrostatic discharging
} 7, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} 7, 26 -- Content-Transfer-Encoding: 7bit
} 7, 26 -- X-Spam-Score: 1.217
} 7, 26 -- X-Spam-Level: * (1.217)
} 7, 26 -- X-Spam-Flag: NO
} 7, 26 -- X-Scanned-By: MIMEDefang 2.42
} ==============================End of -
} Headers==============================

==============================Original
Headers==============================
10, 35 -- From malcolm.haswell-at-sunderland.ac.uk Mon Feb 20 09:22:15 2006
10, 35 -- Received: from max1.sunderland.ac.uk (max1.sunderland.ac.uk
[157.228.29.83])
10, 35 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id
k1KFMEwG029654
10, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Feb 2006
09:22:15 -0600
10, 35 -- Received: (qmail 10493 invoked from network); 20 Feb 2006
15:22:10 -0000
10, 35 -- Received: from localhost (127.0.0.1)
10, 35 -- by max1.sunderland.ac.uk with SMTP; 20 Feb 2006 15:22:10
-0000
10, 35 -- Received: from max1.sunderland.ac.uk ([127.0.0.1])
10, 35 -- by localhost (max1.sunderland.ac.uk [127.0.0.1])
(amavisd-new, port 10024)
10, 35 -- with SMTP id 09230-09 for {Microscopy-at-microscopy.com} ;
10, 35 -- Mon, 20 Feb 2006 15:22:06 +0000 (GMT)
10, 35 -- Received: (qmail 10462 invoked by uid 516); 20 Feb 2006
15:22:06 -0000
10, 35 -- Received: from [157.228.37.117] (HELO hermes.sunderland.ac.uk)
(157.228.37.117)
10, 35 -- by max1.sunderland.ac.uk (qpsmtpd/0.28) with ESMTP; Mon,
20 Feb 2006 15:22:06 +0000
10, 35 -- Received: from sunderland.ac.uk (localhost [127.0.0.1])
10, 35 -- by hermes.sunderland.ac.uk
10, 35 -- (iPlanet Messaging Server 5.2 Patch 2 (built Jul 14 2004))
10, 35 -- with ESMTP id {0IUZ00MBGS0VEP-at-hermes.sunderland.ac.uk} for
10, 35 -- Microscopy-at-microscopy.com; Mon, 20 Feb 2006 15:22:07 +0000
(GMT)
10, 35 -- Received: from [157.228.15.253] by hermes.sunderland.ac.uk
(mshttpd); Mon,
10, 35 -- 20 Feb 2006 15:22:07 +0000 (GMT)
10, 35 -- Date: Mon, 20 Feb 2006 15:22:07 +0000 (GMT)
10, 35 -- From: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk}
10, 35 -- Subject: Re: [Microscopy] TEM film electrostatic discharging
10, 35 -- To: tonygr-at-MIT.EDU, Microscopy MSA {Microscopy-at-microscopy.com}
10, 35 -- Message-id: {b19b04b19e96.b19e96b19b04-at-sunderland.ac.uk}
10, 35 -- MIME-version: 1.0
10, 35 -- X-Mailer: iPlanet Messenger Express 5.2 Patch 2 (built Jul 14
2004)
10, 35 -- Content-type: text/plain; charset=us-ascii
10, 35 -- Content-language: en
10, 35 -- Content-transfer-encoding: 7BIT
10, 35 -- Content-disposition: inline
10, 35 -- X-Accept-Language: en
10, 35 -- Priority: normal
10, 35 -- X-Virus-Scanned: by iCritical at max1.sunderland.ac.uk
==============================End of -
Headers==============================

=======================================================================
This e-mail is intended for the person it is addressed to only. The
information contained in it may be confidential and/or protected by
law. If you are not the intended recipient of this message, you must
not make any use of this information, or copy or show it to any
person. Please contact us immediately to tell us that you have
received this e-mail, and return the original to us. Any use,
forwarding, printing or copying of this message is strictly prohibited.
No part of this message can be considered a request for goods or
services.
=======================================================================


==============================Original Headers==============================
8, 31 -- From richard.beanland-at-bookham.com Mon Feb 20 10:38:12 2006
8, 31 -- Received: from mail78.messagelabs.com (mail78.messagelabs.com [195.245.230.131])
8, 31 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1KGcBCj008019
8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 20 Feb 2006 10:38:11 -0600
8, 31 -- X-VirusChecked: Checked
8, 31 -- X-Env-Sender: richard.beanland-at-bookham.com
8, 31 -- X-Msg-Ref: server-4.tower-78.messagelabs.com!1140453489!7816629!1
8, 31 -- X-StarScan-Version: 5.5.9.1; banners=bookham.com,-,-
8, 31 -- X-Originating-IP: [213.249.209.179]
8, 31 -- Received: (qmail 28862 invoked from network); 20 Feb 2006 16:38:09 -0000
8, 31 -- Received: from unknown (HELO cas-smx-brdg-01.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179)
8, 31 -- by server-4.tower-78.messagelabs.com with SMTP; 20 Feb 2006 16:38:09 -0000
8, 31 -- Received: from cas-smx-02.BOOKHAM.ENTERPRISE.PRI ([10.4.0.223]) by cas-smx-brdg-01.BOOKHAM.ENTERPRISE.PRI with Microsoft SMTPSVC(6.0.3790.211);
8, 31 -- Mon, 20 Feb 2006 16:38:08 +0000
8, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0
8, 31 -- Content-class: urn:content-classes:message
8, 31 -- MIME-Version: 1.0
8, 31 -- Content-Type: text/plain;
8, 31 -- charset="us-ascii"
8, 31 -- Subject: Re: TEM film electrostatic discharging
8, 31 -- Date: Mon, 20 Feb 2006 16:38:08 -0000
8, 31 -- Message-ID: {9645D3E33E4C6548B12A7B25F611533E028190-at-cas-smx-02.BOOKHAM.ENTERPRISE.PRI}
8, 31 -- X-MS-Has-Attach:
8, 31 -- X-MS-TNEF-Correlator:
8, 31 -- Thread-Topic: TEM film electrostatic discharging
8, 31 -- Thread-Index: AcY2MdESLSSjQkN8S4i066gcnb8TQQACVscg
8, 31 -- From: "Richard Beanland" {richard.beanland-at-bookham.com}
8, 31 -- To: {microscopy-at-microscopy.com}
8, 31 -- X-OriginalArrivalTime: 20 Feb 2006 16:38:08.0664 (UTC) FILETIME=[07FDA980:01C6363C]
8, 31 -- Content-Transfer-Encoding: 8bit
8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1KGcBCj008019
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Mon, 20 Feb 2006 13:19:58 -0600
Subject: [Microscopy] TEM: Cell culture preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Advice needed on TEM sample prep for cultured cells.

We are growing skin fibroblasts in culture and would like to examine the cells with transmission electron microscopy. To date, our results have been disappointing. We grew the cells on Thermanox coverslips, fixed for 1 hr at room temp in cacodylate-buffered 1.5% glutaraldehyde, 1.5% paraformaldehyde. After buffer washes, the cells were post-fixed with 1% osmium tetroxide, then washed, dehydrated in acetone and embedded in Epon/Araldite.

The cells look OK in general, but the membranes (both plasma membrane and internal membranes) are all rather fuzzy and indistinct. The quality of the ultrastructure is much poorer that we obtain with tissues prepared using almost identical proceures. One would think that since the cells are only a monolayer, preservation would be excellent.

If you have experience with TEM of cultured cells and have obtained good results, I would appreciate any advice you can give me to get better quality ultrastructure.

Please respond to me directly at katzm-at-health.missouri.edu

Thanks,

Martin L. Katz, Ph.D.
Professor
Ophthalmology, Pathobiology, Neurosciences, Genetics
Mason Eye Institute
University of Missouri School of Medicine
Columbia, Missouri  65212
Phone (573) 882-8480
FAX (573) 884-4100
katzm-at-health.missouri.edu
 http://www.muhealth.org/~ophthalmology/Katz.htm



==============================Original Headers==============================
9, 23 -- From TindallR-at-missouri.edu Mon Feb 20 13:19:57 2006
9, 23 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49])
9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1KJJv9B019566
9, 23 -- for {microscopy-at-microscopy.com} ; Mon, 20 Feb 2006 13:19:57 -0600
9, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830);
9, 23 -- Mon, 20 Feb 2006 13:19:57 -0600
9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 23 -- Content-class: urn:content-classes:message
9, 23 -- MIME-Version: 1.0
9, 23 -- Content-Type: text/plain;
9, 23 -- charset="iso-8859-1"
9, 23 -- Subject: TEM: Cell culture preparation
9, 23 -- Date: Mon, 20 Feb 2006 13:19:56 -0600
9, 23 -- Message-ID: {BA876152E8653240BE8572E897083EE701849D7E-at-UM-EMAIL09.um.umsystem.edu}
9, 23 -- X-MS-Has-Attach:
9, 23 -- X-MS-TNEF-Correlator:
9, 23 -- Thread-Topic: TEM: Cell culture preparation
9, 23 -- Thread-Index: AcY2UqHzjBChwr/YROWHPWjAaQBpkg==
9, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
9, 23 -- To: {microscopy-at-microscopy.com}
9, 23 -- X-OriginalArrivalTime: 20 Feb 2006 19:19:57.0367 (UTC) FILETIME=[A2D42C70:01C63652]
9, 23 -- Content-Transfer-Encoding: 8bit
9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1KJJv9B019566
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Mon, 20 Feb 2006 13:57:20 -0600
Subject: [Microscopy] Re: TEM: Cell culture preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Randy/Martin,

You should be able to dispense with the formalin
(not "paraformaldehyde" -- that's the solid from
which fresh formalin is made). Monolayers of
cells generally do not need formalin.
Add 1% tannic acid to both the glut and osmium.
Mallinckrodt 1764; the monomeric form seems to
work better. You may only need the tannic acid in
one of the fixes, probably the glut, but putting
it in both the fixes won't hurt. You may need to
fix for 2 hours, but one hour should be enough.
Note: this is for high-resolution SEM, where any
holes in the plasma membrane are blatant, and it
works well up to mags } 100kX (and higher).
Also, you could try growing the cells on glass
coverslips, and digesting off the coverslip with
HF after fixation, but that's probably not
relevant to your problem.
Good luck.

Phil

} Advice needed on TEM sample prep for cultured cells.
}
} We are growing skin fibroblasts in culture and
} would like to examine the cells with
} transmission electron microscopy. To date, our
} results have been disappointing. We grew the
} cells on Thermanox coverslips, fixed for 1 hr at
} room temp in cacodylate-buffered 1.5%
} glutaraldehyde, 1.5% paraformaldehyde. After
} buffer washes, the cells were post-fixed with 1%
} osmium tetroxide, then washed, dehydrated in
} acetone and embedded in Epon/Araldite.
}
} The cells look OK in general, but the membranes
} (both plasma membrane and internal membranes)
} are all rather fuzzy and indistinct. The
} quality of the ultrastructure is much poorer
} that we obtain with tissues prepared using
} almost identical proceures. One would think
} that since the cells are only a monolayer,
} preservation would be excellent.
}
} If you have experience with TEM of cultured
} cells and have obtained good results, I would
} appreciate any advice you can give me to get
} better quality ultrastructure.
}
} Please respond to me directly at katzm-at-health.missouri.edu
}
} Thanks,
}
} Martin L. Katz, Ph.D.
} Professor
} Ophthalmology, Pathobiology, Neurosciences, Genetics
} Mason Eye Institute
} University of Missouri School of Medicine
} Columbia, Missouriİ 65212
} Phone (573) 882-8480
} FAX (573) 884-4100
} katzm-at-health.missouri.edu
} İhttp://www.muhealth.org/~ophthalmology/Katz.htm
--
Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
024C Brooks Hall
Mt. Pleasant, MI 48859
(989) 774-3576


==============================Original Headers==============================
5, 25 -- From oshel1pe-at-cmich.edu Mon Feb 20 13:57:20 2006
5, 25 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21])
5, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1KJvK7J029432
5, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Feb 2006 13:57:20 -0600
5, 25 -- Received: from egateb.central.cmich.local ([141.209.15.85])
5, 25 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id k1KKaO4l026727;
5, 25 -- Mon, 20 Feb 2006 15:36:24 -0500
5, 25 -- Received: from [141.209.160.132] ([141.209.160.132]) by egateb.central.cmich.local with Microsoft SMTPSVC(5.0.2195.6713);
5, 25 -- Mon, 20 Feb 2006 14:57:16 -0500
5, 25 -- Mime-Version: 1.0
5, 25 -- Message-Id: {f06230903c01fcdfc338c-at-[141.209.160.132]}
5, 25 -- In-Reply-To: {200602201925.k1KJPv8q027648-at-ns.microscopy.com}
5, 25 -- References: {200602201925.k1KJPv8q027648-at-ns.microscopy.com}
5, 25 -- Date: Mon, 20 Feb 2006 14:57:16 -0500
5, 25 -- To: TindallR-at-missouri.edu
5, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
5, 25 -- Subject: Re: [Microscopy] TEM: Cell culture preparation
5, 25 -- Cc: Microscopy-at-microscopy.com
5, 25 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
5, 25 -- X-OriginalArrivalTime: 20 Feb 2006 19:57:17.0011 (UTC) FILETIME=[D9C2BA30:01C63657]
5, 25 -- X-CanItPRO-Stream: default
5, 25 -- X-Spam-Score: -4 () L_EXCH_MF
5, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21
5, 25 -- Content-Transfer-Encoding: 8bit
5, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1KJvK7J029432
==============================End of - Headers==============================




From: mcauliff-at-umdnj.edu
Date: Mon, 20 Feb 2006 14:25:41 -0600
Subject: [Microscopy] Re: TEM: Cell culture preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

TindallR-at-missouri.edu wrote:

Hi Randy:

Many (and I do mean many) years ago I fixed cultures of retinal
pigment epithelial cells for TEM. I used a routine cacodylate-buffered
glut. fix, no formaldehyde although that should not matter. I did put 1%
tannic acid in the fix, both glut and osmium and it helped show the ECM
but I don' think it should be essential. I grew the cells in ordinary
culture dishes, no thermanox c'slips and embedded in Epon substitute. My
guess is that your membranes look fuzzy due to less than optimal
fixation of lipids (old glut and/or osmium?) or extraction of lipids
(too long in dehydration?). Why are you using acetone and not ethanol?
It is my understanding that acetone removes lipids faster than EtOH but
I have also heard that the converse it true. Certainly too long in
dehydration can degrade ultrastructure. Also, add 2mM Ca ions to the fix
to help stabilize the lipids.

Geoff

}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



==============================Original Headers==============================
7, 32 -- From mcauliff-at-umdnj.edu Mon Feb 20 14:25:41 2006
7, 32 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126])
7, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1KKPfHB006954
7, 32 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 14:25:41 -0600
7, 32 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1])
7, 32 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 058694BDF9
7, 32 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 15:25:41 -0500 (EST)
7, 32 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131])
7, 32 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 60A724BE2D
7, 32 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 15:25:40 -0500 (EST)
7, 32 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu
7, 32 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004))
7, 32 -- id {0IV0009015TECC-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu)
7, 32 -- for microscopy-at-msa.microscopy.com; Mon, 20 Feb 2006 15:25:40 -0500 (EST)
7, 32 -- Received: from [127.0.0.1] ([10.138.2.240])
7, 32 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21
7, 32 -- 2004)) with ESMTP id {0IV0001KL62R6J-at-Polaris.umdnj.edu} ; Mon,
7, 32 -- 20 Feb 2006 15:25:39 -0500 (EST)
7, 32 -- Date: Mon, 20 Feb 2006 15:24:54 -0500
7, 32 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
7, 32 -- Subject: Re: [Microscopy] TEM: Cell culture preparation
7, 32 -- In-reply-to: {200602201921.k1KJLIov021601-at-ns.microscopy.com}
7, 32 -- To: TindallR-at-missouri.edu,
7, 32 -- MicroscopyListserver {microscopy-at-msa.microscopy.com}
7, 32 -- Message-id: {43FA2596.8050405-at-umdnj.edu}
7, 32 -- MIME-version: 1.0
7, 32 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1
7, 32 -- Content-transfer-encoding: 7BIT
7, 32 -- X-Accept-Language: en-us, en
7, 32 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2)
7, 32 -- Gecko/20040804 Netscape/7.2 (ax)
7, 32 -- References: {200602201921.k1KJLIov021601-at-ns.microscopy.com}
==============================End of - Headers==============================




From: mcauliff-at-umdnj.edu
Date: Mon, 20 Feb 2006 15:10:53 -0600
Subject: [Microscopy] spam filters or ??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear List;

I see that several people are using spam filters on this list. I
have just received e-mail from 2 people supposedly on this list asking
me to click on a link to verify that I am really sending them a non-spam
e-mail. No way am I going to click on a link from someone I don't know!
What ARE these people thinking?

Geoff

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



==============================Original Headers==============================
6, 29 -- From mcauliff-at-umdnj.edu Mon Feb 20 15:10:53 2006
6, 29 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124])
6, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1KLArhW017219
6, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 15:10:53 -0600
6, 29 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1])
6, 29 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 1F1151C327F
6, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 16:10:53 -0500 (EST)
6, 29 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131])
6, 29 -- by zix01.umdnj.edu (Proprietary) with ESMTP id BEE40A7B41
6, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 16:10:51 -0500 (EST)
6, 29 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu
6, 29 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004))
6, 29 -- id {0IV000H017WH9Y-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu)
6, 29 -- for microscopy-at-msa.microscopy.com; Mon, 20 Feb 2006 16:10:51 -0500 (EST)
6, 29 -- Received: from [127.0.0.1] ([10.138.2.240])
6, 29 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21
6, 29 -- 2004)) with ESMTP id {0IV0001G880C6J-at-Polaris.umdnj.edu} for
6, 29 -- microscopy-at-msa.microscopy.com; Mon, 20 Feb 2006 16:07:24 -0500 (EST)
6, 29 -- Date: Mon, 20 Feb 2006 16:06:39 -0500
6, 29 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
6, 29 -- Subject: spam filters or ??
6, 29 -- To: MicroscopyListserver {microscopy-at-msa.microscopy.com}
6, 29 -- Message-id: {43FA2F5F.7050807-at-umdnj.edu}
6, 29 -- MIME-version: 1.0
6, 29 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1
6, 29 -- Content-transfer-encoding: 7BIT
6, 29 -- X-Accept-Language: en-us, en
6, 29 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2)
6, 29 -- Gecko/20040804 Netscape/7.2 (ax)
==============================End of - Headers==============================




From: david.knecht-at-uconn.edu
Date: Mon, 20 Feb 2006 15:32:19 -0600
Subject: [Microscopy] TIRF setup

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We recently saw a TIRF system demonstrated, and like other laser
based TIRF systems, it seems to use a small peripheral arc aperture
to allow the laser light through to part of the outside periphery of
the 1.45NA objective. Why would you not use a complete ring annulus
instead of an arc to illuminate the entire outside periphery of the
objective? If you simply put the correct sized annulus into the field
stop in the fluorescence path, would you get TIRF? Also, what is the
theoretical advantage of using a laser as opposed to an arc
illuminator. Thanks- Dave

Dr. David Knecht
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)


==============================Original Headers==============================
3, 19 -- From david.knecht-at-uconn.edu Mon Feb 20 15:32:18 2006
3, 19 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204])
3, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1KLWITb026790
3, 19 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 15:32:18 -0600
3, 19 -- Received: from [137.99.47.163] (d47h163.public.uconn.edu [137.99.47.163])
3, 19 -- by mail2.uits.uconn.edu (8.11.6/8.11.6) with ESMTP id k1KLW0m26333
3, 19 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 16:32:00 -0500
3, 19 -- Mime-Version: 1.0 (Apple Message framework v746.2)
3, 19 -- Content-Transfer-Encoding: 7bit
3, 19 -- Message-Id: {8E45F94A-D08D-4926-9B2F-ECE8BF9D3920-at-uconn.edu}
3, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
3, 19 -- To: Microscopy-at-msa.microscopy.com
3, 19 -- From: David Knecht {david.knecht-at-uconn.edu}
3, 19 -- Subject: TIRF setup
3, 19 -- Date: Mon, 20 Feb 2006 16:32:02 -0500
3, 19 -- X-Mailer: Apple Mail (2.746.2)
3, 19 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information.
3, 19 -- X-UConn-MailScanner: Found to be clean
3, 19 -- X-UConn-MailScanner-SpamCheck:
==============================End of - Headers==============================




From: rcommon-at-msu.edu
Date: Mon, 20 Feb 2006 16:01:59 -0600
Subject: [Microscopy] TEM film electrostatic discharging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

For the floor, there is a spray that can be put on it to temporarily
stop electrostatic buildup. I'm sorry, but I can't remember the name of
it, but I have used it in the past.

For electronic use, I found that taking my shoes off solves static
discharge problems. There is enough moisture on my feet to stop any
discharges.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: malcolm.haswell-at-sunderland.ac.uk
[mailto:malcolm.haswell-at-sunderland.ac.uk]
Sent: Monday, February 20, 2006 7:28 AM
To: Walck-at-SouthBayTech.com

Tony

one thing that occurs to me is that you will probably have vinyl
flooring in your darkroom. This may be a major source of static and it
may be possible to get some advice on low static flooring. I know we
had some installed in one of our microscope cubicles and it didn't seem
to be tremendously expensive at the time.

If you think that the floor is part of the problem then it might be
worth checking whether the soles of your shoes are man made (create
static) or leather.

If you want to go for a tried and tested electronics industry route
look for ionisers (rather than de-ionisers) I did a simple Google
search on ioniser and static and came up with a few using
keywords "ionisers static"
such as http://www.buystatic.com/static_eliminators.htm?ioniser.htm

I was hoping that you could get away with a domestic ioniser - the sort
they sell to simulate sea or mountain air and supposedly gets rid of
headaches. But apparently they aren't much good at eliminating static
whereas the industrial ones are.

Good luck

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK

e-mail: malcolm.haswell-at-sunderland.ac.uk

----- Original Message -----
X-from: tonygr-at-MIT.EDU

Some of the solutions suggested seem to be based on the idea that the
operator is picking up the static charge. I think the problem is much more
likely to be caused by static on the film being discharged while unloading
the exposed negatives. I now unload film wearing rubber gloves and rarely
get a static discharge pattern on my film.

Ralph Common
Dept. of Physiology
Michigan State University


==============================Original Headers==============================
3, 25 -- From rcommon-at-msu.edu Mon Feb 20 16:01:59 2006
3, 25 -- Received: from sys15.mail.msu.edu (sys15.mail.msu.edu [35.9.75.115])
3, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1KM1xUa013444
3, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Feb 2006 16:01:59 -0600
3, 25 -- Received: from [35.9.122.125] (helo=emlab)
3, 25 -- by sys15.mail.msu.edu with esmtpsa (Exim 4.52 #1)
3, 25 -- (TLSv1:RC4-MD5:128)
3, 25 -- id 1FBJ6U-0001zU-V8
3, 25 -- for Microscopy-at-microscopy.com; Mon, 20 Feb 2006 17:01:59 -0500
3, 25 -- From: "Ralph Common" {rcommon-at-msu.edu}
3, 25 -- To: {Microscopy-at-microscopy.com}
3, 25 -- Subject: TEM film electrostatic discharging
3, 25 -- Date: Mon, 20 Feb 2006 17:02:37 -0500
3, 25 -- Message-ID: {001401c63669$5dc89410$7d7a0923-at-msu.edu}
3, 25 -- MIME-Version: 1.0
3, 25 -- Content-Type: text/plain;
3, 25 -- charset="iso-8859-1"
3, 25 -- Content-Transfer-Encoding: 7bit
3, 25 -- X-Priority: 3 (Normal)
3, 25 -- X-MSMail-Priority: Normal
3, 25 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0)
3, 25 -- In-Reply-To: {200602201528.k1KFSxtR005488-at-ns.microscopy.com}
3, 25 -- Importance: Normal
3, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1506
3, 25 -- X-Virus: None found by Clam AV
==============================End of - Headers==============================




From: frank.karl-at-degussa.com
Date: Tue, 21 Feb 2006 07:09:26 -0600
Subject: [Microscopy] Re: viaWWW: microtome section thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Interesting question....
As I understand it the colors seen in thin sections are caused by
destructive interference. The equation:
Wavelength = 2nt/m describes the color seen. n = refractive index of film
t is thickness (nm)
M is any integer.

If so it seems that as refractive index decreases, wavelength will also
decrease for the same thickness film.

I hope this help and thank you for giving me a chance to thumb through my
old reference books, it brought back memories....

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
messages attached to it may contain information that is confidential or
legally privileged. If you are not the intended recipient, or a person
responsible for delivering it to the intended recipient, you are hereby
notified that you must not read this transmission and that any disclosure,
copying, printing, distribution or use of any of the information contained
in or attached to this transmission is STRICTLY PROHIBITED. If you have
received this transmission in error, please immediately notify the sender
by telephone or return e-mail and delete the original transmission and its
attachments without reading or saving in any manner. Thank you.


==============================Original Headers==============================
9, 18 -- From frank.karl-at-degussa.com Tue Feb 21 07:09:25 2006
9, 18 -- Received: from mailout1.degussa.com (mailout1.degussa.com [193.100.56.173])
9, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1LD9OXV002077
9, 18 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 07:09:25 -0600
9, 18 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [172.20.6.74])
9, 18 -- by mailout1.degussa.com (8.13.3/8.13.3/Debian-6) with SMTP id k1LD9B0P024169;
9, 18 -- Tue, 21 Feb 2006 14:09:17 +0100
9, 18 -- In-Reply-To: {200602172210.k1HMA6nj031675-at-ns.microscopy.com}
9, 18 -- Subject: Re: [Microscopy] viaWWW: microtome section thickness
9, 18 -- To: leis-at-biochem.mpg.de, microscopy-at-msa.microscopy.com
9, 18 -- X-Mailer: Lotus Notes Release 6.5.2 June 01, 2004
9, 18 -- Message-ID: {OFC1F9625B.D0797714-ON8525711C.004779CF-8525711C.004839F5-at-degussa.com}
9, 18 -- From: frank.karl-at-degussa.com
9, 18 -- Date: Tue, 21 Feb 2006 08:08:54 -0500
9, 18 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at
9, 18 -- 02/21/2006 07:09:18 AM
9, 18 -- MIME-Version: 1.0
9, 18 -- Content-type: text/plain; charset=US-ASCII
==============================End of - Headers==============================




From: David.Patton-at-uwe.ac.uk
Date: Tue, 21 Feb 2006 08:55:34 -0600
Subject: [Microscopy] spam filters or ??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I ignore these suspicious requests as well. I guess those folk don't
get much mail now.

Dave

-----Original Message-----
X-from: mcauliff-at-umdnj.edu [mailto:mcauliff-at-umdnj.edu]
Sent: 20 February 2006 21:15
To: David Patton

Dear List;

I see that several people are using spam filters on this list. I
have just received e-mail from 2 people supposedly on this list asking
me to click on a link to verify that I am really sending them a non-spam

e-mail. No way am I going to click on a link from someone I don't know!
What ARE these people thinking?

Geoff

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



==============================Original
Headers==============================
6, 29 -- From mcauliff-at-umdnj.edu Mon Feb 20 15:10:53 2006
6, 29 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU
[130.219.34.124])
6, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
k1KLArhW017219
6, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006
15:10:53 -0600
6, 29 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1])
6, 29 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id
1F1151C327F
6, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006
16:10:53 -0500 (EST)
6, 29 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU
[130.219.34.131])
6, 29 -- by zix01.umdnj.edu (Proprietary) with ESMTP id
BEE40A7B41
6, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006
16:10:51 -0500 (EST)
6, 29 -- Received: from conversion-daemon.Polaris.umdnj.edu by
Polaris.umdnj.edu
6, 29 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004))
6, 29 -- id {0IV000H017WH9Y-at-Polaris.umdnj.edu} (original mail from
mcauliff-at-umdnj.edu)
6, 29 -- for microscopy-at-msa.microscopy.com; Mon, 20 Feb 2006 16:10:51
-0500 (EST)
6, 29 -- Received: from [127.0.0.1] ([10.138.2.240])
6, 29 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02
(built Oct 21
6, 29 -- 2004)) with ESMTP id {0IV0001G880C6J-at-Polaris.umdnj.edu} for
6, 29 -- microscopy-at-msa.microscopy.com; Mon, 20 Feb 2006 16:07:24 -0500
(EST)
6, 29 -- Date: Mon, 20 Feb 2006 16:06:39 -0500
6, 29 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
6, 29 -- Subject: spam filters or ??
6, 29 -- To: MicroscopyListserver {microscopy-at-msa.microscopy.com}
6, 29 -- Message-id: {43FA2F5F.7050807-at-umdnj.edu}
6, 29 -- MIME-version: 1.0
6, 29 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1
6, 29 -- Content-transfer-encoding: 7BIT
6, 29 -- X-Accept-Language: en-us, en
6, 29 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US;
rv:1.7.2)
6, 29 -- Gecko/20040804 Netscape/7.2 (ax)
==============================End of -
Headers==============================


This incoming email to UWE has been independently scanned for viruses
and any virus detected has been removed using McAfee anti-virus software



This email has been independently scanned for viruses and any virus software has been removed using McAfee anti-virus software


==============================Original Headers==============================
20, 33 -- From David.Patton-at-uwe.ac.uk Tue Feb 21 08:55:34 2006
20, 33 -- Received: from mailapp01.uwe.ac.uk (mailapp01.uwe.ac.uk [164.11.132.61])
20, 33 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1LEtVGe013689
20, 33 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 08:55:33 -0600
20, 33 -- Received: from (164.11.132.60) by mailapp01.uwe.ac.uk via smtp
20, 33 -- id 4a43_94e119d4_a2ea_11da_89e4_0002b3c946e4;
20, 33 -- Tue, 21 Feb 2006 14:58:56 +0000
20, 33 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk
20, 33 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121])
20, 33 -- by mta01.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24
20, 33 -- 2005)) with ESMTP id {0IV1006AELGIO1-at-mta01.uwe.ac.uk} for
20, 33 -- microscopy-at-msa.microscopy.com; Tue, 21 Feb 2006 14:55:30 +0000 (GMT)
20, 33 -- Date: Tue, 21 Feb 2006 14:55:29 +0000
20, 33 -- From: David Patton {David.Patton-at-uwe.ac.uk}
20, 33 -- Subject: RE: [Microscopy] spam filters or ??
20, 33 -- To: microscopy-at-msa.microscopy.com
20, 33 -- Message-id: {F247F674896BE243AD8263C5280E2BDB022BB804-at-egen-uwe01}
20, 33 -- MIME-version: 1.0
20, 33 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5.6944.0
20, 33 -- Content-type: text/plain; charset=us-ascii
20, 33 -- Content-class: urn:content-classes:message
20, 33 -- Thread-topic: [Microscopy] spam filters or ??
20, 33 -- Thread-index: AcY2YzRIJ0lBMgRET9a4rhY9wkWYYgAk2+uQ
20, 33 -- X-MS-Has-Attach:
20, 33 -- X-MS-TNEF-Correlator:
20, 33 -- X-NAI-Spam-Level: *
20, 33 -- X-NAI-Spam-Score: 1.5
20, 33 -- X-NAI-Spam-Rules: 2 Rules triggered
20, 33 -- NAI_ZIP_CODE=4, BAYES_00=-2.5
20, 33 -- X-NAIMIME-Disclaimer: 1
20, 33 -- X-NAIMIME-Modified: 1
20, 33 -- Content-Transfer-Encoding: 8bit
20, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1LEtVGe013689
==============================End of - Headers==============================




From: hyi-at-emory.edu
Date: Tue, 21 Feb 2006 10:06:29 -0600
Subject: [Microscopy] Re: TEM: Cell culture preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Randy:

           I have heard other people report situation like this and I,
too, have experienced the same thing. Often time (not always) monolayer
cells showed very little membrane contrast, even though the tissue
processed same way had no problem. The problem with monolayer cells
seemed random regardless what types of cells were being processed. More
interestingly, I have not seen this problem with cell suspensions. In
the past, I tried to use freshly made OsO4 once when I had low membrane
contrast problem with monolayer cells, and that helped. But I still do
not understand why the problem only occurs in monolayer cells. I do not
think it is a reagent penetration issue, nor a problem of inadequate
processing protocol. Is it possible that some kind of coating material
used in culture makes it harder for OsO4 to react with lipid
molecules? 
Thank you.

Hong
Emory EM




==============================Original Headers==============================
5, 22 -- From hyi-at-emory.edu Tue Feb 21 10:06:29 2006
5, 22 -- Received: from virginia.cc.emory.edu (virginia.cc.emory.edu [170.140.8.222])
5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1LG6S2K024016
5, 22 -- for {microscopy-at-microscopy.com} ; Tue, 21 Feb 2006 10:06:28 -0600
5, 22 -- Received: from [170.140.232.202] (localhost [127.0.0.1])
5, 22 -- by virginia.cc.emory.edu (8.13.4/8.13.4) with ESMTP id k1LG6OIf028249
5, 22 -- for {microscopy-at-microscopy.com} ; Tue, 21 Feb 2006 11:06:28 -0500 (EST)
5, 22 -- Mime-Version: 1.0 (Apple Message framework v622)
5, 22 -- Message-Id: {5a5232b7e407dc1cfb2b84946528c088-at-emory.edu}
5, 22 -- Content-Type: text/plain;
5, 22 -- charset=ISO-8859-1;
5, 22 -- format=flowed
5, 22 -- To: microscopy-at-microscopy.com
5, 22 -- From: Hong Yi {hyi-at-emory.edu}
5, 22 -- Subject: Re: [Microscopy] TEM: Cell culture preparation
5, 22 -- Date: Tue, 21 Feb 2006 11:06:21 -0500
5, 22 -- X-Mailer: Apple Mail (2.622)
5, 22 -- X-imss-version: 2.037
5, 22 -- X-imss-result: Passed
5, 22 -- X-imss-approveListMatch: *-at-emory.edu
5, 22 -- Content-Transfer-Encoding: 8bit
5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1LG6S2K024016
==============================End of - Headers==============================




From: krueger.eugene-at-mayo.edu
Date: Tue, 21 Feb 2006 10:56:47 -0600
Subject: [Microscopy] Re: TEM: Cell culture preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I process monolayers of cells frequently for TEM. The problem of low membrane contrast is due to the lipids being extracted away during dehydration and embedding. Even membranes "stabilized" with OsO4 can be extracted with the long processing times used during "standard fixations". I typically dehydrate in EtOH for 1-2 minutes per EtOH grade, with my samples dehydrated from 100% water to 100% EtOH in 15-20 minutes. I also keep the time in liquid resin to a minimum...my whole embedding protocol takes less than 3 hours. After I shortened my times considerably, my membranes started looking very nice and crisp!
Good Luck
-Eugene

Eugene W. A. Krueger
Sr. Research Technologist in Cell Biology II
Center for Basic Research in Digestive Diseases
Mayo Clinic and Foundation
(507)284-0580 (lab)
(507)284-0762 (fax)
krueger.eugene-at-mayo.edu




} Hi, Randy:
}
} I have heard other people report situation like this and I,
} too, have experienced the same thing. Often time (not always) monolayer
} cells showed very little membrane contrast, even though the tissue
} processed same way had no problem. The problem with monolayer cells
} seemed random regardless what types of cells were being processed. More
} interestingly, I have not seen this problem with cell suspensions. In
} the past, I tried to use freshly made OsO4 once when I had low membrane
} contrast problem with monolayer cells, and that helped. But I still do
} not understand why the problem only occurs in monolayer cells. I do not
} think it is a reagent penetration issue, nor a problem of inadequate
} processing protocol. Is it possible that some kind of coating material
} used in culture makes it harder for OsO4 to react with lipid
} molecules?
} Thank you.
}
} Hong
} Emory EM
}
}
}
}
} ==============================Original Headers==============================
} 5, 22 -- From hyi-at-emory.edu Tue Feb 21 10:06:29 2006
} 5, 22 -- Received: from virginia.cc.emory.edu (virginia.cc.emory.edu [170.140.8.222])
} 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1LG6S2K024016
} 5, 22 -- for {microscopy-at-microscopy.com} ; Tue, 21 Feb 2006 10:06:28 -0600
} 5, 22 -- Received: from [170.140.232.202] (localhost [127.0.0.1])
} 5, 22 -- by virginia.cc.emory.edu (8.13.4/8.13.4) with ESMTP id k1LG6OIf028249
} 5, 22 -- for {microscopy-at-microscopy.com} ; Tue, 21 Feb 2006 11:06:28 -0500 (EST)
} 5, 22 -- Mime-Version: 1.0 (Apple Message framework v622)
} 5, 22 -- Message-Id: {5a5232b7e407dc1cfb2b84946528c088-at-emory.edu}
} 5, 22 -- Content-Type: text/plain;
} 5, 22 -- charset=ISO-8859-1;
} 5, 22 -- format=flowed
} 5, 22 -- To: microscopy-at-microscopy.com
} 5, 22 -- From: Hong Yi {hyi-at-emory.edu}
} 5, 22 -- Subject: Re: [Microscopy] TEM: Cell culture preparation
} 5, 22 -- Date: Tue, 21 Feb 2006 11:06:21 -0500
} 5, 22 -- X-Mailer: Apple Mail (2.622)
} 5, 22 -- X-imss-version: 2.037
} 5, 22 -- X-imss-result: Passed
} 5, 22 -- X-imss-approveListMatch: *-at-emory.edu
} 5, 22 -- Content-Transfer-Encoding: 8bit
} 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1LG6S2K024016
} ==============================End of - Headers==============================
}
}

==============================Original Headers==============================
6, 20 -- From krueger.eugene-at-mayo.edu Tue Feb 21 10:56:47 2006
6, 20 -- Received: from mail1.mayo.edu (mail1.mayo.edu [129.176.212.12])
6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1LGuloa001993
6, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Feb 2006 10:56:47 -0600
6, 20 -- Received: from mhro1a.mayo.edu ([129.176.212.53])
6, 20 -- by mail1.mayo.edu with ESMTP; 21 Feb 2006 10:56:46 -0600
6, 20 -- X-BrightmailFiltered: true
6, 20 -- X-Brightmail-Tracker: AAAAAA==
6, 20 -- Received: from excsrv01.mayo.edu (excsrv01.mayo.edu [129.176.235.101]) by mhro1a.mayo.edu with ESMTP for Microscopy-at-microscopy.com; Tue, 21 Feb 2006 10:56:46 -0600
6, 20 -- Received: by excsrv01.mayo.edu with Internet Mail Service (5.5.2657.72)
6, 20 -- id {FMHXTMZG} ; Tue, 21 Feb 2006 10:53:21 -0600
6, 20 -- Message-Id: {FC1B895EA707A940A6BD5F74106FAA9A269497-at-excsrv80.mayo.edu}
6, 20 -- From: "Krueger, Eugene W." {krueger.eugene-at-mayo.edu}
6, 20 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
6, 20 -- Subject: Re: TEM: Cell culture preparation
6, 20 -- Date: Tue, 21 Feb 2006 10:56:42 -0600
6, 20 -- MIME-Version: 1.0
6, 20 -- X-Mailer: Internet Mail Service (5.5.2657.72)
6, 20 -- Content-Type: text/plain;
6, 20 -- charset="iso-8859-1"
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Tue, 21 Feb 2006 13:32:32 -0600
Subject: [Microscopy] Re: TEM film electrostatic discharging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Feb 18, 2006, at 5:54 AM, tonygr-at-MIT.EDU wrote:

} This is a perennial problem, I know, but does anyone have any
} effective "pet" solutions to the problem of charging and subsequent
} electrostatic arcing of TEM film in the extremely dry air we get in
} New England typically in the winter, and some other people get at
} other seasons of the year?
}
} The discharges can occur at almost any stage of the handling, from
} getting the film out of the vendor's packing, loading and unloading in
} the microscope carriers and loading in the developing racks (we use
} Lucite ones). We have considered a humidifier in the darkroom, but
} that would involve blocking off the ventilation (we have make-up air
} positively blown into to room and exhaust actively sucked out) to
} prevent our moisture from being simply blown away.
}
} Any hints would be welcome, but switching to digital imaging is one
} that is not going to happen.
}
Dear Tony,
When removing the film from the envelopes, keep it in a pack, so none
of the films rubs along any other, then lift the cardboard without
rubbing. If you can maintain contact with a grounded metal
surface--the water pipes are good--this will help a lot. Try not to
let your lab coat (or other clothing for that matter) to move along
your body or other items of clothing. Always separate films by bending
the top one away from the others, then lifting. Trade your lucite
racks for metal ones. These procedures reduced, but did not completely
eliminate, the problem when I was in Albany. When using LoDose film in
total darkness, I could at least see the discharges when they occurred.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
4, 22 -- From tivol-at-caltech.edu Tue Feb 21 13:32:32 2006
4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1LJWVHu013187
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 13:32:32 -0600
4, 22 -- Received: from localhost (earth-dog [192.168.1.3])
4, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id 5719E35BB9
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 11:32:31 -0800 (PST)
4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21])
4, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id 2D62435A54
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 11:32:30 -0800 (PST)
4, 22 -- Mime-Version: 1.0 (Apple Message framework v623)
4, 22 -- In-Reply-To: {200602181354.k1IDsSQp003552-at-ns.microscopy.com}
4, 22 -- References: {200602181354.k1IDsSQp003552-at-ns.microscopy.com}
4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
4, 22 -- Message-Id: {8eab6d7bd81e0fcbc6fc662ef0c1c9fa-at-caltech.edu}
4, 22 -- Content-Transfer-Encoding: 7bit
4, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
4, 22 -- Subject: Re: [Microscopy] TEM film electrostatic discharging
4, 22 -- Date: Tue, 21 Feb 2006 11:40:34 -0800
4, 22 -- To: microscopy-at-msa.microscopy.com
4, 22 -- X-Mailer: Apple Mail (2.623)
4, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.3.3
==============================End of - Headers==============================




From: gwe-at-ufl.edu
Date: Tue, 21 Feb 2006 14:18:41 -0600
Subject: [Microscopy] MT-1 for free

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We have a vintage Sorvall MT-1 ultramicrotome available for the cost of
shipping. Manual included. Has no stereomicroscope with it. Great for
semithin plastic sections. All manual operation. It is headed for the
junk pile if no one adopts it.

--
Gregory W. Erdos, Ph.D,
Assistant Director, Biotechnology Program
Scientific Director, EM Core Lab
P.O. Box 118525
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
Phone: 352-392-1295
Fax: 352-846-0251


==============================Original Headers==============================
3, 22 -- From gwe-at-ufl.edu Tue Feb 21 14:18:40 2006
3, 22 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149])
3, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1LKIeL1023349
3, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 14:18:40 -0600
3, 22 -- Received: from [10.227.60.63] (empc14419.dhcp.clas.ufl.edu [10.227.60.63])
3, 22 -- (authenticated bits=0)
3, 22 -- by smtp.ufl.edu (8.13.4/8.13.4/2.5.1) with ESMTP id k1LKIacU4337810
3, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 15:18:37 -0500
3, 22 -- Message-ID: {43FB759D.6050002-at-ufl.edu}
3, 22 -- Date: Tue, 21 Feb 2006 15:18:37 -0500
3, 22 -- From: Greg Erdos {gwe-at-ufl.edu}
3, 22 -- User-Agent: Mozilla Thunderbird 1.0.7 (Windows/20050923)
3, 22 -- X-Accept-Language: en-us, en
3, 22 -- MIME-Version: 1.0
3, 22 -- To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com}
3, 22 -- Subject: MT-1 for free
3, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
3, 22 -- Content-Transfer-Encoding: 7bit
3, 22 -- X-Spam-Status: hits=-0.904, required=5, tests=BAYES_30
3, 22 -- X-UFL-Spam-Status: hits=-0.904, required=5, tests=BAYES_30
3, 22 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/)
3, 22 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/)
==============================End of - Headers==============================




From: gwe-at-ufl.edu
Date: Tue, 21 Feb 2006 14:45:20 -0600
Subject: [Microscopy] Free M-1 has already been claimed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The free MT-1 has already found a new home.

--
Gregory W. Erdos, Ph.D,
Assistant Director, Biotechnology Program
Scientific Director, EM Core Lab
P.O. Box 118525
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
Phone: 352-392-1295
Fax: 352-846-0251


==============================Original Headers==============================
3, 22 -- From gwe-at-ufl.edu Tue Feb 21 14:45:20 2006
3, 22 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149])
3, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1LKjKxX000550
3, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 14:45:20 -0600
3, 22 -- Received: from [10.227.60.63] (empc14419.dhcp.clas.ufl.edu [10.227.60.63])
3, 22 -- (authenticated bits=0)
3, 22 -- by smtp.ufl.edu (8.13.4/8.13.4/2.5.1) with ESMTP id k1LKjHAH3805302
3, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 15:45:18 -0500
3, 22 -- Message-ID: {43FB7BDE.8080103-at-ufl.edu}
3, 22 -- Date: Tue, 21 Feb 2006 15:45:18 -0500
3, 22 -- From: Greg Erdos {gwe-at-ufl.edu}
3, 22 -- User-Agent: Mozilla Thunderbird 1.0.7 (Windows/20050923)
3, 22 -- X-Accept-Language: en-us, en
3, 22 -- MIME-Version: 1.0
3, 22 -- To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com}
3, 22 -- Subject: Free M-1 has already been claimed
3, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
3, 22 -- Content-Transfer-Encoding: 7bit
3, 22 -- X-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED
3, 22 -- X-UFL-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED
3, 22 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/)
3, 22 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/)
==============================End of - Headers==============================




From: krueger.eugene-at-mayo.edu
Date: Tue, 21 Feb 2006 14:47:11 -0600
Subject: [Microscopy] embedding schedule for monolayers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I was asked to post my embedding schedule, so here it is:

When embedding monolayers (~60 - 90% confluent) in epoxy resin (I prefer Quetol 651) I follow this schedule:

graded series of EtOH, 25%, 50%, 70%, 95%, 100%, 100%, total dehydration time 15-20'
100%EtOH:Quetol 651 (1:1), ~25'
100% Quetol 651, ~50'
100% Quetol 651, ~100'
fresh 100% Quetol 651, then into 60C oven

I do my processing in a 6 or 12 well plate that sits on a shelf in the hood (NOT on a rotator), and I change to a new 6 or 12 well plate between changes of 100% EtOH. I also use minimal volumes of resin to decrease extraction of lipid components. If you use a more viscous resin, times may need to be adjusted longer.
Good Luck!

-Eugene
Eugene W. A. Krueger
Sr. Research Technologist in Cell Biology II
Center for Basic Research in Digestive Diseases
Mayo Clinic and Foundation
(507)284-0580 (lab)
(507)284-0762 (fax)
krueger.eugene-at-mayo.edu

==============================Original Headers==============================
5, 20 -- From krueger.eugene-at-mayo.edu Tue Feb 21 14:47:11 2006
5, 20 -- Received: from mail2.mayo.edu (mail2.mayo.edu [129.176.212.15])
5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1LKlBv7003576
5, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Feb 2006 14:47:11 -0600
5, 20 -- Received: from mhro1a.mayo.edu ([129.176.212.53])
5, 20 -- by mail2.mayo.edu with ESMTP; 21 Feb 2006 14:47:10 -0600
5, 20 -- X-BrightmailFiltered: true
5, 20 -- X-Brightmail-Tracker: AAAAAA==
5, 20 -- Received: from excsrv01.mayo.edu (excsrv01.mayo.edu [129.176.235.101]) by mhro1a.mayo.edu with ESMTP for Microscopy-at-microscopy.com; Tue, 21 Feb 2006 14:47:10 -0600
5, 20 -- Received: by excsrv01.mayo.edu with Internet Mail Service (5.5.2657.72)
5, 20 -- id {FMHXW34R} ; Tue, 21 Feb 2006 14:43:45 -0600
5, 20 -- Message-Id: {FC1B895EA707A940A6BD5F74106FAA9A269498-at-excsrv80.mayo.edu}
5, 20 -- From: "Krueger, Eugene W." {krueger.eugene-at-mayo.edu}
5, 20 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
5, 20 -- Subject: embedding schedule for monolayers
5, 20 -- Date: Tue, 21 Feb 2006 14:47:07 -0600
5, 20 -- MIME-Version: 1.0
5, 20 -- X-Mailer: Internet Mail Service (5.5.2657.72)
5, 20 -- Content-Type: text/plain;
5, 20 -- charset="iso-8859-1"
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Tue, 21 Feb 2006 18:39:29 -0600
Subject: [Microscopy] Re: viaWWW: scale bar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Feb 18, 2006, at 2:32 PM, soleimanij-at-tbzmed.ac.ir wrote:

} we are using A LEO 906 TEM, scale bar is not registered in the
} microfilms. We are having problem to calculating the real sizes. any
} help in this matter is appreciated.
}
Dear Jafar,
I would calibrate the magnification(s) of interest either with a
cross-grating replica or, preferably, a Mag*I*Cal, both of which are
available from many supply houses. If you need sizes on prints, there
is a calibration slide that can be photographed in an enlarger, with
which you can determine the ratio of print sizes to negative sizes; I
don't know where to get this, but it must be widely available.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
4, 22 -- From tivol-at-caltech.edu Tue Feb 21 18:39:28 2006
4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1M0dSPK023528
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 18:39:28 -0600
4, 22 -- Received: from localhost (water-dog [192.168.1.26])
4, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id 63DDE359F6
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 16:39:28 -0800 (PST)
4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21])
4, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id AF21035C10
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 16:39:16 -0800 (PST)
4, 22 -- Mime-Version: 1.0 (Apple Message framework v623)
4, 22 -- In-Reply-To: {200602182232.k1IMW6Zs027921-at-ns.microscopy.com}
4, 22 -- References: {200602182232.k1IMW6Zs027921-at-ns.microscopy.com}
4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
4, 22 -- Message-Id: {7bf40a7674c34bd31ebb3e601deaaae1-at-caltech.edu}
4, 22 -- Content-Transfer-Encoding: 7bit
4, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
4, 22 -- Subject: Re: [Microscopy] viaWWW: scale bar
4, 22 -- Date: Tue, 21 Feb 2006 16:47:18 -0800
4, 22 -- To: microscopy-at-msa.microscopy.com
4, 22 -- X-Mailer: Apple Mail (2.623)
4, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.3.3
==============================End of - Headers==============================




From: milesd-at-us.ibm.com
Date: Tue, 21 Feb 2006 20:05:08 -0600
Subject: [Microscopy] Re: spam filters or ??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

It is MOST likely that those emails did not come from the people they look
like they came from.

Darrell




mcauliff-at-umdnj.ed
u
To
02/20/2006 04:11 Darrell Miles/Fishkill/IBM-at-IBMUS
PM cc

Subject
Please respond to [Microscopy] spam filters or ??
mcauliff-at-umdnj.ed
u











----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Dear List;

I see that several people are using spam filters on this list. I
have just received e-mail from 2 people supposedly on this list asking
me to click on a link to verify that I am really sending them a non-spam
e-mail. No way am I going to click on a link from someone I don't know!
What ARE these people thinking?

Geoff

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



==============================Original
Headers==============================
6, 29 -- From mcauliff-at-umdnj.edu Mon Feb 20 15:10:53 2006
6, 29 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124])
6, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
k1KLArhW017219
6, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006
15:10:53 -0600
6, 29 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1])
6, 29 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id
1F1151C327F
6, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006
16:10:53 -0500 (EST)
6, 29 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU
[130.219.34.131])
6, 29 -- by zix01.umdnj.edu (Proprietary) with ESMTP id
BEE40A7B41
6, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006
16:10:51 -0500 (EST)
6, 29 -- Received: from conversion-daemon.Polaris.umdnj.edu by
Polaris.umdnj.edu
6, 29 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004))
6, 29 -- id {0IV000H017WH9Y-at-Polaris.umdnj.edu} (original mail from
mcauliff-at-umdnj.edu)
6, 29 -- for microscopy-at-msa.microscopy.com; Mon, 20 Feb 2006 16:10:51
-0500 (EST)
6, 29 -- Received: from [127.0.0.1] ([10.138.2.240])
6, 29 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02
(built Oct 21
6, 29 -- 2004)) with ESMTP id {0IV0001G880C6J-at-Polaris.umdnj.edu} for
6, 29 -- microscopy-at-msa.microscopy.com; Mon, 20 Feb 2006 16:07:24 -0500
(EST)
6, 29 -- Date: Mon, 20 Feb 2006 16:06:39 -0500
6, 29 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
6, 29 -- Subject: spam filters or ??
6, 29 -- To: MicroscopyListserver {microscopy-at-msa.microscopy.com}
6, 29 -- Message-id: {43FA2F5F.7050807-at-umdnj.edu}
6, 29 -- MIME-version: 1.0
6, 29 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1
6, 29 -- Content-transfer-encoding: 7BIT
6, 29 -- X-Accept-Language: en-us, en
6, 29 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US;
rv:1.7.2)
6, 29 -- Gecko/20040804 Netscape/7.2 (ax)
==============================End of -
Headers==============================



==============================Original Headers==============================
23, 27 -- From milesd-at-us.ibm.com Tue Feb 21 20:05:07 2006
23, 27 -- Received: from e3.ny.us.ibm.com (e3.ny.us.ibm.com [32.97.182.143])
23, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1M257Q0001451
23, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 20:05:07 -0600
23, 27 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236])
23, 27 -- by e3.ny.us.ibm.com (8.12.11/8.12.11) with ESMTP id k1M255Vc016162
23, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 21:05:05 -0500
23, 27 -- Received: from d01av01.pok.ibm.com (d01av01.pok.ibm.com [9.56.224.215])
23, 27 -- by d01relay04.pok.ibm.com (8.12.10/NCO/VERS6.8) with ESMTP id k1M2562i220526
23, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 21:05:06 -0500
23, 27 -- Received: from d01av01.pok.ibm.com (loopback [127.0.0.1])
23, 27 -- by d01av01.pok.ibm.com (8.12.11/8.13.3) with ESMTP id k1M255LU008116
23, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 21:05:05 -0500
23, 27 -- Received: from d01ml076.pok.ibm.com (d01ml076.pok.ibm.com [9.56.229.50])
23, 27 -- by d01av01.pok.ibm.com (8.12.11/8.12.11) with ESMTP id k1M2540P008094
23, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 21:05:04 -0500
23, 27 -- In-Reply-To: {200602202111.k1KLBUjW018639-at-ns.microscopy.com}
23, 27 -- Subject: Re: [Microscopy] spam filters or ??
23, 27 -- To: Microscopy-at-MSA.Microscopy.Com
23, 27 -- X-Mailer: Lotus Notes Release 7.0 HF85 November 04, 2005
23, 27 -- Message-ID: {OF22CCB88F.52878652-ON8525711D.000B000E-8525711D.000B7274-at-us.ibm.com}
23, 27 -- From: Darrell Miles {milesd-at-us.ibm.com}
23, 27 -- Date: Tue, 21 Feb 2006 21:05:03 -0500
23, 27 -- X-MIMETrack: Serialize by Router on D01ML076/01/M/IBM(Release 6.5.4FP2 HF4|December 20, 2005) at
23, 27 -- 02/21/2006 21:05:04
23, 27 -- MIME-Version: 1.0
23, 27 -- Content-type: text/plain; charset=US-ASCII
==============================End of - Headers==============================




From: mayas003-at-yahoo.com
Date: Tue, 21 Feb 2006 22:58:21 -0600
Subject: [Microscopy] Scanner for TEM Micrographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings:

I'm looking for a reliable scanner for TEM
Micrographs. For the past 4 years I used an old HP
scanner that was very good with 35mm and TEM
micrographs, unfortunate this scanner is no longer
working, now I need a scanner that can capture a
reliable image for routine everyday micrographs; in
order to shorten darkroom time. Any subjection?

Thanks in advance

Omayra Velez
Life Cell
Branchburg, NJ


==============================Original Headers==============================
5, 18 -- From mayas003-at-yahoo.com Tue Feb 21 22:58:21 2006
5, 18 -- Received: from web84109.mail.dcn.yahoo.com (web84109.mail.dcn.yahoo.com [209.73.179.120])
5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1M4wK2N012775
5, 18 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 22:58:20 -0600
5, 18 -- Received: (qmail 63646 invoked by uid 60001); 22 Feb 2006 04:58:20 -0000
5, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
5, 18 -- s=s1024; d=yahoo.com;
5, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
5, 18 -- b=l3I3qltvjW7C7MFLKskZiUVjJbPQ1cydee1f6xuI3x0YEOtyb1XLN+xZX7pO4ne8HOWyjClZfM4jfomwRvPZ1aIimFvPffyyv5mgzkS65fZVHYy/qLK30Kq3Ft6gNcfeVuPoHIlCg5oBNKSj3juJH9BgGJ/Hx0brg5OPd0fZ8gY= ;
5, 18 -- Message-ID: {20060222045820.63644.qmail-at-web84109.mail.dcn.yahoo.com}
5, 18 -- Received: from [68.239.240.252] by web84109.mail.dcn.yahoo.com via HTTP; Tue, 21 Feb 2006 20:58:20 PST
5, 18 -- Date: Tue, 21 Feb 2006 20:58:20 -0800 (PST)
5, 18 -- From: Omayra Velez {mayas003-at-yahoo.com}
5, 18 -- Subject: Scanner for TEM Micrographs
5, 18 -- To: Microscopy-at-MSA.Microscopy.Com
5, 18 -- MIME-Version: 1.0
5, 18 -- Content-Type: text/plain; charset=iso-8859-1
5, 18 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: mcauliff-at-umdnj.edu
Date: Wed, 22 Feb 2006 09:02:53 -0600
Subject: [Microscopy] Re: Scanner for TEM Micrographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Omayra,

Like microscopes, it really is the more you pay the better the result (even
my kids £60 DX5 microscope has 250x magnification and a CMOS 'camera').

However, from my experience even going to £2,000 for a scanner, the result
of a negative or slide is sometimes not as good as the original if you
really start magnifying it up (although I haven't tried the latest 4000 to
5000 dpi top of the range Nikon slide scanners). This is mainly due to film
grain size optical effects that even Digital GEM doesn't seem to wholly
eliminate (it is just a software image processing system even if embedded
into the scanner hardware & optimised for the scanner). Grain size optical
effects are such that a 400ASA negative colour scan at 2,700dpi can produce
an appallingly unusable image that image processing can't really save -
garbage in garbage out (whereas a reflective scan of the 6x4" print produces
a very reasonable A4 image). Higher resolutions approaching or exceeding the
grain size of the file (e.g. 4000 dpi and above) are supposed to reduce this
problem considerably but Nikon type slide scanners are expensive and very
limited in negative size options. In practice many problems in image quality
are as much due to ease of magnifying a digital image compared to the
negative - a few clicks and you have a 'print' the size of a wall.

However the scanned slide image from lower ASA 50-100 slides at 4000dpi is
probably better on the VDU than most standard (not enlargements) prints made
from the negative/slide and it can easily go up to A3 in output to a
printer, which is probably all that really matters (and you still have the
negative in the likely event the next generation scanners will get even
better for the price). However enlargement prints from the negative may be
better than a magnified scanned image. Naturally if you want to zoom in on a
negative, it would have been better to do this on the TEM in the first place
and take another picture instead (wise after the event).

In practice it might also be that our eyes prefer a fuzzy analogue gradation
of colour rather than the very defined little squares of a pixellated image
at the same resolution. We are also good at discerning contrast. Also
remember that digital camera's often do some image processing during capture
(e.g. noise reduction, colour correction and sometimes even sharpen) so you
have to work on the image after scanning to get the same result. Top of the
range scanner software and hardware can do this 'automatically' using
calibration targets and so forth (e.g. Digital GEM, SHO, ICE and Silverfast
Ai). This scan processing can triple scan times to 10 minutes or more, but
can save far more than that on manual Photoshop type processing times
afterwards (and make a better job of it). Note that we can only discern 191
grey levels so 8-bit (256 grey levels) B&W scans be fine for most uses
except perhaps image analysis - and this really reduced image file size.

So the scanned images can look very good at A3 and on a 22" monitor, and if
your negative archive is going the way of my collection of 1950's 35mm home
slides and literally disintegrating into brown mush, anything is an
improvement. Surprisingly the twain software can also have an effect on
scanned quality in some cases (cheaper slide scanners often benefit from
using Silverfast SE over the cheaper bundled software).

To scan many large negatives at high resolution via a 5000dpi Creo type
hi-resolution flatbed scanner would need an investment of £12k to £45 and
the massive files would be hard to manage and archive without image size
reduction and compression which renders the whole procedure rather
pointless. Given the cost it would seem to be preferable to pay someone to
scan the odd few negatives you need scanned at this resolution with their
Creo. Hi-resolution drum scanners up to 1500dpi cost nearer £70k but if your
collection is something like NASA's archive that price can be well worth
paying (see their results at http://grin.hq.nasa.gov). A 2k x 2k or greater
pixel image digital camera system for a TEM costs around £20k to £70k.

You can get pretty good results from the new breed of sub-£1,000 flatbed
scanners though, particularly with large format negatives (i.e. TEM). Have a
look at the reviews of these semi-pro flatbed scanners on the net (around
£300 to 800 to buy). Below is an excellent link for the Canon 9950F that
could be used for 4x5" or 9 x 6cm film. It also compares the results with
the similar Epson 4990 Photo.

http://www.photo-i.co.uk

I use the Canon 9950F (£260) at home for 35mm negatives and slides. The
Canon's main weakness is that it's twain interface can only scan to the
frame sizes (i.e. not to my square Kodak 125 slides, where it chops on the
top and bottom of the slides to fit it to 35mm - a real pain). With
Silverfast SE [twain] also provided, you can scan any film size. However
Canon won't share the FARE dust removal technology with Silverfast so FARE
isn't supported - not a problem with B&W negatives as FARE and ICE only work
with colour - although you can scan B&W negatives in 'colour' with FARE/ICE
and convert to B&W in Photoshop - but 'results may vary'. I have upgraded to
Silverfast Ai for $115 for my Canon 9950F which adds in auto multi-slide
scans (but not FARE). Canon no longer manufacture dedicated slide scanners
as they feel this flatbed is as good.

At work I use the Epson 4990 (£300). It has a better twain interface and has
an A4 negative scan feature that can scan any smaller negatives in multiples
if they don't fit the standard frames provided. Silverfast SE also provided
supports ICE infra-red dust removal - but dust removal can reduce image
quality a little if the negative isn't that dusty. I use a standard rubber
bulb to blow dust off before scanning - air jets canisters are gone in a day
and can squirt propellant over the film. It's image quality is identical to
Canon 9950F for 35mm slides, although the Epson can only scan 8 slides at
one go to the Canon's 12. Scan time is similar for both (very slow with
FARE/ICE - about 10 minutes a 35mm slide). However both these flatbeds are
really 2,400 dpi not 4800 dpi as claimed - if you scan 35mm at 4800 dpi the
image quality is virtually identical the 2,400 dpi scan. However you can't
regularly scan full TEM at 4,800 dpi and above as the image size would be
near 1 Gb - we scan at 800 to 1,200 dpi mainly - although you can of course
scan small areas at 2,400 dpi resolution for 'enlargements'. All uses say
they are happy with the TEM scan quality.

If you keep the negative anyway, I would have thought being able to print to
A3 size is adequate for most purposes. The cheap Canon 9950F flatbed has
replaced my 2,700 dpi SCSI Scanwit 2740s dedicated slide scanner at home
(largely as it's so much faster and easier to use). I have to say the image
quality of these flatbeds is a little out of focus (or 'soft' as we call it)
at full magnification, but the careful use of USM (unsharp mask) in
Photoshop can improve this a lot. But they are fine up to A3 at least (from
a 35mm slide). Flatbed scans always need a little more Twain and post scan
tweeking than dedicated film scanners. Leave things like USM and colour
balance to Photoshop but use the twain interface to set things like
brightness in dark negatives and dust ICE/FARE removal.

These scanners can come with expensive Silverfast Ai and targets though
(sometimes as a Pro version) or you can upgrade at silverfast.com. But a lot
of this is for accurate colour (not TEM's strong point) - although
Silverfast is a powerful & complicated Twain interface.

When going from 2,700 dpi of the old slide/negative scanners up to the 4,000
dpi of modern Nikon type slide/negative scanners many users report far
better image quality, and put it down to reduced effects from grain aliasing
e.g. http://hardware.mcse.ms/message144915.html
and presumably the same will be true of TEM negatives and the latest 4,000
dpi semi-pro flatbed scanners (that can take 4"x5") as well. At these
resolutions film grain is also very apparent though, as 35mm film grain has
a lower dpi.

There's also dedicated & cheap large film scanners like the Epson F3200
going to 4x5" film but again the resolution of the F3200 is quoted at 'only'
3200dpi, plus it got a duff review :
http://www.photo-i.co.uk/Reviews/interactive/Scanners/Epson_F3200/page-1.htm

In fact it is probably a little better for image quality (prior to USM) than
the flatbeds for large B&W negatives, but you may be limited to the frame
sizes so check if you have 6x9cm negatives rather than proper ¼ plate. It
seems that it can scan any size smaller than the largest frame - but
certainly there's no A4 negative or reflective scanning.

Have a look at:
http://www.photoscientia.co.uk/Grain.htm
for discussions of grain size.

Have a look at
http://www.datamind.co.uk/merchant/resolution.htm
for some chat on pixel and image file sizes.

Hope this is of some use.

Regards

Keith


PS. This a bit large to post on the listerver, but it gave me something to
do while travelling for 4 hours on the train to & from work each day. This
follows on from similar threads about scanner a few months ago.


----- Original Message -----
X-from: {mayas003-at-yahoo.com}
To: {keith.morris-at-ucl.ac.uk}
Sent: Wednesday, February 22, 2006 5:02 AM

I put my EM negatives on a light box, mask off all other areas with
black paper (so 'extra' light does not mislead the light meter), copy
with my digital camera, then invert the image to a positive and convert
to grayscale in PhotoShop. Fast, easy and excellent results.

Geoff

mayas003-at-yahoo.com wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



==============================Original Headers==============================
7, 31 -- From mcauliff-at-umdnj.edu Wed Feb 22 09:02:53 2006
7, 31 -- Received: from zix02.umdnj.edu (zix02.UMDNJ.EDU [130.219.34.125])
7, 31 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1MF2qZ5007257
7, 31 -- for {microscopy-at-msa.microscopy.com} ; Wed, 22 Feb 2006 09:02:52 -0600
7, 31 -- Received: from zix02.umdnj.edu (ZixVPM [127.0.0.1])
7, 31 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 586FC4BE3C
7, 31 -- for {microscopy-at-msa.microscopy.com} ; Wed, 22 Feb 2006 09:02:52 -0600 (CST)
7, 31 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131])
7, 31 -- by zix02.umdnj.edu (Proprietary) with ESMTP id 2EB6F4BE34
7, 31 -- for {microscopy-at-msa.microscopy.com} ; Wed, 22 Feb 2006 09:02:51 -0600 (CST)
7, 31 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu
7, 31 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004))
7, 31 -- id {0IV300E01GF9ER-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu)
7, 31 -- for microscopy-at-msa.microscopy.com; Wed, 22 Feb 2006 10:02:50 -0500 (EST)
7, 31 -- Received: from [127.0.0.1] ([10.138.2.240])
7, 31 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21
7, 31 -- 2004)) with ESMTP id {0IV300H8HGBMQ5-at-Polaris.umdnj.edu} ; Wed,
7, 31 -- 22 Feb 2006 09:59:46 -0500 (EST)
7, 31 -- Date: Wed, 22 Feb 2006 09:59:01 -0500
7, 31 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
7, 31 -- Subject: Re: [Microscopy] Scanner for TEM Micrographs
7, 31 -- In-reply-to: {200602220459.k1M4xdY3014788-at-ns.microscopy.com}
7, 31 -- To: mayas003-at-yahoo.com, MicroscopyListserver {microscopy-at-msa.microscopy.com}
7, 31 -- Message-id: {43FC7C35.5050601-at-umdnj.edu}
7, 31 -- MIME-version: 1.0
7, 31 -- Content-type: text/plain; format=flowed; charset=us-ascii
7, 31 -- Content-transfer-encoding: 7BIT
7, 31 -- X-Accept-Language: en-us, en
7, 31 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2)
7, 31 -- Gecko/20040804 Netscape/7.2 (ax)
7, 31 -- References: {200602220459.k1M4xdY3014788-at-ns.microscopy.com}
==============================End of - Headers==============================




From: heckman-at-bgnet.bgsu.edu
Date: Wed, 22 Feb 2006 14:22:27 -0600
Subject: [Microscopy] Fwd: Re: TEM: Cell culture preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I am not sure why these would not be legitimate. I have gotten such requests that cited my message of some date and some subject, so they already have my e-mail address. I can easily envision an anti-spam service where a person must respond to such a challenge to get added to a white-list of senders. I have replied to a couple of those posts as instructed and have not been asked to re-register.

However, I also sent them a note that such a service is not very compatible with membership in a list server like this. Everyone who ever posts to the list will get challenged to register their e-mail address. I doubt that many will. And if I declined to register and continued to get these registration requests, I would probably add that person to my junk mailers list.

If someone halfway around the world doesn't want to accept the knowledge that gets shared via this list then that is their problem. They should not use such a challenge system on the e-mail address they use for this or other listservers.

Warren
________________________________________
X-from: milesd-at-us.ibm.com [mailto:milesd-at-us.ibm.com]
Sent: Tue 2/21/2006 8:05 PM
To: wesaia-at-iastate.edu

Hi

from previous replies on the list it seems to come down to about 3-4
basic types of film scanner:
1. Very expensive dedicated drum scanners
2. Standard dedicated film scanners like the Nikon
3. A combined flatbed and glassless scanner (Agfa used to make the Duo-
scan)
4. Standard flatbed scanner with glass.

I think that Keith has covered everything but the 3. combined scanner.
I use a Microtek Scanmaker 8700 and find it useful because there is no
glass between the optics and the film which should reduce Newton's
Rings, dust and finger marks. The new Microtek is cheaper and more
powerful than mine was. It handles true 3200 x 6400 dpi scans, 4.2 Dmax
negatives, 48 bit colour and sells for under 700 UK pounds or about 600
US dollars. I think it still comes with Silversoft scanning software
and Digital ICE (scratch and dust reducing software/hardware). This
should give up to 16x enlargements with reasonably dense negatives up
to sizes of 10x 8 inches or more (about 4 of our 4489 negatives at
once).
See http://www.microtekusa.com/smi900.html

A lot depends on the size and nature of your negatives as to whether
they will fit into a particular scanner. If you produce a lot of dense
negatives (usually not a problem with biological samples) then maybe
you will need more than 4.2 Dmax.

I don't know of any of the off-the-shelf scanners which come with a
ready made holder for the larger e.m. negatives so you may have to get
something made up even if it's just out of bits of cardboard or plastic.

Good luck

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk



----- Original Message -----
X-from: keith.morris-at-ucl.ac.uk



While your observation is valid, in some cases those subscribing to
listservers have no control over how spam is filtered. Our email goes
through the corporate servers located over a mile away and through filters
set up by the corporate IT group. Some of their filters are rather
exasperating for us in the microscopy group. For example, ALL embedded
images from incoming emails are deleted by the corporate filters. What we
get in its place is the marker [IMAGE]. If we need to have images sent to
us, they must come as attachments. Again, this is out of our control. So
too with spam. Fortunately our corporate filters direct anything it thinks
is spam into a "spam folder". We can go through the emails in the folder
at our leisure and if it is from someone we know we can mark it and add it
to the list of allowed senders. But unlike the systems you are describing,
we, as the recipient, must accept the email. Nothing is sent to the poster
as far as I can tell. This is one of the reasons I like having the
[Microscopy] tag on the subject line. It makes it easier to pick out the
real emails in my horrendous list of spam in the morning.

My point in all this is we should not be too hard on those posters since
they may not have any control over the situation and may not even know it
is happening.


Sharon Goresh
Chemist
Engelhard Corp
Research Center
Iselin, NJ



wesaia-at-iastate.ed
u
To
02/22/06 10:13 AM sharon.goresh-at-engelhard.com
cc

Please respond to Subject
wesaia-at-iastate.ed [Microscopy] Re: spam filters or ??
u