Microscopy ListServer Archives  


File Requested = 0604.txt
Retrival Software Version=NJZ07060908

From: hinmeigeng-at-hotmail.com
Date: Sat, 1 Apr 2006 04:30:21 -0600
Subject: [Microscopy] Polyester Fibre Sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings All !

A colleague wants to prepare bundles of polyester fibres for SEM by
embedding and cutting with a Microm HM325 microtome using a steel knife.
The idea is to examine the cut surface of the block to study the
cross-sectional shape of the fibres.

Could you please recommend the best type of embedding material for the
purpose? It should not contain aggressive solvents, or be cured at too high
a temperature.

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------



==============================Original Headers==============================
6, 21 -- From hinmeigeng-at-hotmail.com Sat Apr 1 04:30:21 2006
6, 21 -- Received: from hotmail.com (bay101-f34.bay101.hotmail.com [64.4.56.44])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k31AUKTF011498
6, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sat, 1 Apr 2006 04:30:21 -0600
6, 21 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC;
6, 21 -- Sat, 1 Apr 2006 02:30:20 -0800
6, 21 -- Message-ID: {BAY101-F34425505819B73D3958F3ECAD70-at-phx.gbl}
6, 21 -- Received: from 64.4.56.200 by by101fd.bay101.hotmail.msn.com with HTTP;
6, 21 -- Sat, 01 Apr 2006 10:30:17 GMT
6, 21 -- X-Originating-IP: [86.128.212.193]
6, 21 -- X-Originating-Email: [hinmeigeng-at-hotmail.com]
6, 21 -- X-Sender: hinmeigeng-at-hotmail.com
6, 21 -- Reply-To: R.H.Olley-at-reading.ac.uk
6, 21 -- From: "Robert H. Olley" {hinmeigeng-at-hotmail.com}
6, 21 -- To: Microscopy-at-MSA.Microscopy.Com
6, 21 -- Cc: R.H.Olley-at-reading.ac.uk
6, 21 -- Subject: Polyester Fibre Sectioning
6, 21 -- Date: Sat, 01 Apr 2006 10:30:17 +0000
6, 21 -- Mime-Version: 1.0
6, 21 -- Content-Type: text/plain; format=flowed
6, 21 -- X-OriginalArrivalTime: 01 Apr 2006 10:30:20.0902 (UTC) FILETIME=[471A6860:01C65577]
==============================End of - Headers==============================




From: sally.stowe-at-anu.edu.au
Date: Sat, 1 Apr 2006 08:15:07 -0600
Subject: [Microscopy] Protrain SEM Workshop Canberra June 2006

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


SEM Course - Operation and Basic Maintenance.

The 2006 Workshop run by Steve Chapman (Protrain Ltd, UK) will be held in
Canberra at the Australian National University Electron Microscopy Unit.

The workshop will run over 5 days from the 12th to the 16th June 2006,
and will cover SEM operation and basic maintenance, with some EDXA and FIB
work.

The course in 2006 will be run in association with Anaspec cc Australia.
The cost will be $AUS800 for the 5-day course, which includes morning and
afternoon tea but no other meals or accommodation.

Contactfor details and bookings: Ruth O'Loughlin, Ruth-at-anaspec.co.za
Canberra travel and accommodation suggestions can be found on

These highly recommended courses are enjoyable, intensive and interactive.
Participants gain experience in getting the best possible performance from
a range of SEMs. Professional microscopists and actual and aspiring
"power users" are the main clientele, some people have so much fun they
come back to do the course twice!

Accommodation: at ANU http://accom.anu.edu.au/
Other local accommodation :
http://canberra.citysearch.com.au/section/visitor-guide/

Transport to Canberra - air, train, bus or
road...http://canberra.citysearch.com.au/feature/51/gettingThere.html

Sally Stowe

--
Dr SJ Stowe
ANU Electron Microscopy Unit
http://www.anu.edu.au/EMU/index.html

==============================Original Headers==============================
11, 26 -- From sally.stowe-at-anu.edu.au Sat Apr 1 08:15:07 2006
11, 26 -- Received: from mail.rsbs.anu.edu.au (mail.rsbs.anu.edu.au [150.203.72.70])
11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k31EF6D2023777
11, 26 -- for {microscopy-at-microscopy.com} ; Sat, 1 Apr 2006 08:15:07 -0600
11, 26 -- Received: from mail.rsbs.anu.edu.au (mail.rsbs.anu.edu.au [127.0.0.1])
11, 26 -- by mail.rsbs.anu.edu.au (Postfix) with SMTP
11, 26 -- id BD6AAF441E1; Sun, 2 Apr 2006 01:15:22 +1100 (EST)
11, 26 -- Received: from 203.113.234.139
11, 26 -- (SquirrelMail authenticated user u8411377)
11, 26 -- by mail.rsbs.anu.edu.au with HTTP;
11, 26 -- Sun, 2 Apr 2006 00:15:22 +1000 (EST)
11, 26 -- Message-ID: {1412.203.113.234.139.1143900922.squirrel-at-mail.rsbs.anu.edu.au}
11, 26 -- Date: Sun, 2 Apr 2006 00:15:22 +1000 (EST)
11, 26 -- Subject: Protrain SEM Workshop Canberra June 2006
11, 26 -- From: sally.stowe-at-anu.edu.au
11, 26 -- To: microscopy-at-microscopy.com, austem-at-anu.edu.au
11, 26 -- User-Agent: SquirrelMail/1.4.2-1
11, 26 -- MIME-Version: 1.0
11, 26 -- Content-Type: text/plain;charset=iso-8859-1
11, 26 -- Content-Transfer-Encoding: 8bit
11, 26 -- X-Priority: 3
11, 26 -- Importance: Normal
11, 26 -- X-RSBS-MailScanner-Information: Please contact the ISP for more information
11, 26 -- X-RSBS-MailScanner: Found to be clean
11, 26 -- X-RSBS-MailScanner-SpamScore: s
11, 26 -- X-MailScanner-From: sally.stowe-at-anu.edu.au
==============================End of - Headers==============================




From: Colin.Veitch-at-csiro.au
Date: Sat, 1 Apr 2006 15:31:33 -0600
Subject: [Microscopy] Polyester Fibre Sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

We routinely observe fibre (all kinds - polymers, wools, cotton etc) cross sections in the sem, but rather than embedding them in resin, we feed the the bundle through the heat shrink tubing used in the electronics industry (as narrow as possible), shrink the tubing and then cut them using a sharp blade (we have found injector blades to be the best) on a Hardy microtome or similiar.

We have found this to be far less time consuming than embedding in a resin with very similar results.

Cheers

Colin Veitch
CSIRO Textile and Fibre Technology
Geelong, Australia


-----Original Message-----
X-from: hinmeigeng-at-hotmail.com [mailto:hinmeigeng-at-hotmail.com]
Sent: Sat 1/04/2006 9:30 PM
To: Veitch, Colin (TFT, Geelong)
Cc:

Greetings All !

A colleague wants to prepare bundles of polyester fibres for SEM by
embedding and cutting with a Microm HM325 microtome using a steel knife.
The idea is to examine the cut surface of the block to study the
cross-sectional shape of the fibres.

Could you please recommend the best type of embedding material for the
purpose? It should not contain aggressive solvents, or be cured at too high
a temperature.

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------



==============================Original Headers==============================
6, 21 -- From hinmeigeng-at-hotmail.com Sat Apr 1 04:30:21 2006
6, 21 -- Received: from hotmail.com (bay101-f34.bay101.hotmail.com [64.4.56.44])
6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k31AUKTF011498
6, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sat, 1 Apr 2006 04:30:21 -0600
6, 21 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC;
6, 21 -- Sat, 1 Apr 2006 02:30:20 -0800
6, 21 -- Message-ID: {BAY101-F34425505819B73D3958F3ECAD70-at-phx.gbl}
6, 21 -- Received: from 64.4.56.200 by by101fd.bay101.hotmail.msn.com with HTTP;
6, 21 -- Sat, 01 Apr 2006 10:30:17 GMT
6, 21 -- X-Originating-IP: [86.128.212.193]
6, 21 -- X-Originating-Email: [hinmeigeng-at-hotmail.com]
6, 21 -- X-Sender: hinmeigeng-at-hotmail.com
6, 21 -- Reply-To: R.H.Olley-at-reading.ac.uk
6, 21 -- From: "Robert H. Olley" {hinmeigeng-at-hotmail.com}
6, 21 -- To: Microscopy-at-MSA.Microscopy.Com
6, 21 -- Cc: R.H.Olley-at-reading.ac.uk
6, 21 -- Subject: Polyester Fibre Sectioning
6, 21 -- Date: Sat, 01 Apr 2006 10:30:17 +0000
6, 21 -- Mime-Version: 1.0
6, 21 -- Content-Type: text/plain; format=flowed
6, 21 -- X-OriginalArrivalTime: 01 Apr 2006 10:30:20.0902 (UTC) FILETIME=[471A6860:01C65577]
==============================End of - Headers==============================





==============================Original Headers==============================
20, 31 -- From Colin.Veitch-at-csiro.au Sat Apr 1 15:31:24 2006
20, 31 -- Received: from vic-MTAout5.csiro.au (vic-MTAout5.csiro.au [150.229.64.42])
20, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k31LVL26020169
20, 31 -- for {Microscopy-at-microscopy.com} ; Sat, 1 Apr 2006 15:31:24 -0600
20, 31 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=Edr5xS+CNVwM1ZkcxZmzJTgVjkzESAVg+fBUD1PNJAo8d4oeaa9LLRw8nWUUBbDf/4eDecwdLsZ/L3OE5ysfljcOqt8MqrZIw1UdUsSO6VdhPf9assrLCiPpf+1hCYjZ;
20, 31 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56])
20, 31 -- by vic-MTAout5.csiro.au with ESMTP; 02 Apr 2006 07:31:20 +1000
20, 31 -- X-IronPort-AV: i="4.03,154,1141563600";
20, 31 -- d="scan'208"; a="73618511:sNHT60718480"
20, 31 -- Received: from exvicn2-mel.nexus.csiro.au ([138.194.3.62]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713);
20, 31 -- Sun, 2 Apr 2006 07:31:19 +1000
20, 31 -- Received: from exvic5-gex.nexus.csiro.au ([138.194.194.6]) by exvicn2-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713);
20, 31 -- Sun, 2 Apr 2006 07:31:19 +1000
20, 31 -- X-MIMEOLE: Produced By Microsoft Exchange V6.0.6603.0
20, 31 -- Content-Class: urn:content-classes:message
20, 31 -- MIME-Version: 1.0
20, 31 -- Content-Type: text/plain;
20, 31 -- charset="Windows-1252"
20, 31 -- Subject: RE: [Microscopy] Polyester Fibre Sectioning
20, 31 -- Date: Sun, 2 Apr 2006 07:31:17 +1000
20, 31 -- Message-ID: {32CDDDAA7161394599F002549491574902480B-at-exvic5-gex.nexus.csiro.au}
20, 31 -- X-MS-Has-Attach:
20, 31 -- X-MS-TNEF-Correlator:
20, 31 -- Thread-Topic: [Microscopy] Polyester Fibre Sectioning
20, 31 -- Thread-Index: AcZVd07U+l155sLxTiK9SMcIEvwpgAAW0W4b
20, 31 -- References: {200604011030.k31AUT1P011648-at-ns.microscopy.com}
20, 31 -- From: {Colin.Veitch-at-csiro.au}
20, 31 -- To: {hinmeigeng-at-hotmail.com} , {Microscopy-at-microscopy.com}
20, 31 -- X-OriginalArrivalTime: 01 Apr 2006 21:31:19.0827 (UTC) FILETIME=[9DAA1E30:01C655D3]
20, 31 -- Content-Transfer-Encoding: 8bit
20, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k31LVL26020169
==============================End of - Headers==============================




From: zaluzec-at-microscopy.com
Date: Sun, 2 Apr 2006 12:22:12 -0500
Subject: [Microscopy] Injector Blade?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


For general reference and because I'm just curious, what is an
"injector blade" and when would you use it in a microtome
instead of either diamond or glass blades.

Or is this blade not used with microtomes?



Nestor
Your Friendly Neighborhood SysOp

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Sun Apr 2 12:22:12 2006
6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k32HMBkP025186
6, 11 -- for {microscopy-at-microscopy.com} ; Sun, 2 Apr 2006 12:22:11 -0500
6, 11 -- Mime-Version: 1.0
6, 11 -- Message-Id: {p06110401c055b822c831-at-[206.69.208.22]}
6, 11 -- Date: Sun, 2 Apr 2006 12:22:10 -0500
6, 11 -- To: microscopy-at-microscopy.com
6, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
6, 11 -- Subject: Injector Blade?
6, 11 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: schooley-at-mcn.org
Date: Sun, 2 Apr 2006 12:59:09 -0500
Subject: [Microscopy] Microscopy] LM - OX3

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Some of you listers enjoy using the inexpensive children's digital
microscope, the QX3, as a home hobby item. It's gone thru several
changes, but it's still available, with improved software. There's a
new software package, available at
http://www.edhsw.com/mixscope/index.html

I have no interest in the company, and no personal opinion on the
product; I just want you to have fun...
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO

==============================Original Headers==============================
2, 16 -- From schooley-at-mcn.org Sun Apr 2 12:59:08 2006
2, 16 -- Received: from dns3.mcn.org (dns3.mcn.org [216.150.240.32])
2, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k32Hx8s4002705
2, 16 -- for {microscopy-at-microscopy.com} ; Sun, 2 Apr 2006 12:59:08 -0500
2, 16 -- Received: from [66.42.18.122] (helo=[10.0.1.2])
2, 16 -- by dns3.mcn.org with esmtpa (Exim 4.60)
2, 16 -- (envelope-from {schooley-at-mcn.org} )
2, 16 -- id IX3WMJ-000LAN-39; Sun, 02 Apr 2006 10:59:07 -0700
2, 16 -- Mime-Version: 1.0
2, 16 -- Message-Id: {a06200701c055be59a1a0-at-[10.0.1.2]}
2, 16 -- Date: Sun, 2 Apr 2006 10:56:56 -0700
2, 16 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com}
2, 16 -- From: Caroline Schooley {schooley-at-mcn.org}
2, 16 -- Subject: Microscopy] LM - OX3
2, 16 -- Cc: diamondsci-at-charter.net, schooley-at-mac.com
2, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: schooley-at-mcn.org
Date: Sun, 2 Apr 2006 12:59:11 -0500
Subject: [Microscopy] Re: Injector Blade?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Caroline

--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO

==============================Original Headers==============================
4, 18 -- From schooley-at-mcn.org Sun Apr 2 12:59:09 2006
4, 18 -- Received: from dns3.mcn.org (dns3.mcn.org [216.150.240.32])
4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k32Hx7mY002704;
4, 18 -- Sun, 2 Apr 2006 12:59:08 -0500
4, 18 -- Received: from [66.42.18.122] (helo=[10.0.1.2])
4, 18 -- by dns3.mcn.org with esmtpa (Exim 4.60)
4, 18 -- (envelope-from {schooley-at-mcn.org} )
4, 18 -- id IX3WMH-000LAN-2T; Sun, 02 Apr 2006 10:59:05 -0700
4, 18 -- Mime-Version: 1.0
4, 18 -- Message-Id: {a06200700c055bc5528ab-at-[10.0.1.2]}
4, 18 -- In-Reply-To: {200604021729.k32HTNCJ002059-at-ns.microscopy.com}
4, 18 -- References: {200604021729.k32HTNCJ002059-at-ns.microscopy.com}
4, 18 -- Date: Sun, 2 Apr 2006 10:45:51 -0700
4, 18 -- To: zaluzec-at-microscopy.com
4, 18 -- From: Caroline Schooley {schooley-at-mcn.org}
4, 18 -- Subject: Re: [Microscopy] Injector Blade?
4, 18 -- Cc: "Microscopy Listserver" {microscopy-at-microscopy.com}
4, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: phillipst-at-missouri.edu
Date: Sun, 2 Apr 2006 13:15:18 -0500
Subject: [Microscopy] Re: Injector Blade?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

One can use disposable injector blades from a dispenser quite similar to
what used to be used for razor blades for shaving in old-fashioned packages
for either paraffin microtomy or cryostat work. I do not believe there is
an application for ultramicrotomy where they would take the place of a
diamond or glass blade. Tom


At 12:23 PM 04/02/06, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



==============================Original Headers==============================
10, 21 -- From PhillipsT-at-missouri.edu Sun Apr 2 13:15:17 2006
10, 21 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49])
10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k32IFHVc021895
10, 21 -- for {Microscopy-at-msa.microscopy.com} ; Sun, 2 Apr 2006 13:15:17 -0500
10, 21 -- Received: from um-exproto9.um.umsystem.edu ([207.160.151.148]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830);
10, 21 -- Sun, 2 Apr 2006 13:15:14 -0500
10, 21 -- Received: from phillips-dell.missouri.edu ([128.206.81.132]) by um-exproto9.um.umsystem.edu over TLS secured channel with Microsoft SMTPSVC(6.0.3790.1830);
10, 21 -- Sun, 2 Apr 2006 13:15:12 -0500
10, 21 -- Message-Id: {6.0.0.22.2.20060402131021.056c5830-at-pop.missouri.edu}
10, 21 -- X-Sender: phillipst-at-pop.missouri.edu
10, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 6.0.0.22
10, 21 -- Date: Sun, 02 Apr 2006 13:13:08 -0500
10, 21 -- To: {zaluzec-at-microscopy.com}
10, 21 -- From: Tom Phillips {phillipst-at-missouri.edu}
10, 21 -- Subject: Re: [Microscopy] Injector Blade?
10, 21 -- Cc: Microscopy-at-msa.microscopy.com
10, 21 -- In-Reply-To: {200604021723.k32HNT3p026753-at-ns.microscopy.com}
10, 21 -- References: {200604021723.k32HNT3p026753-at-ns.microscopy.com}
10, 21 -- Mime-Version: 1.0
10, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
10, 21 -- X-OriginalArrivalTime: 02 Apr 2006 18:15:13.0248 (UTC) FILETIME=[62A66600:01C65681]
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Sun, 2 Apr 2006 20:24:51 -0500
Subject: [Microscopy] Re: Injector Blade?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Ah yes, how fondly i remember my old Schick injector. First razor -
took it to university with me. Then they got chromium edged blades,
Then someone decided we needed two blades, and the rest was history.
Now we can get 5 blades, with a motor of some sort, too. In spite of
the nostalgia, must confess that it did cut my face up all to heck and
gone. But i still have the old razor....


While I'm tired of cutting my face up, I still use injector blades in
the blade holder for my old ultratome III to trim blocks.


paul




Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Work Phone: 204-789-3313
Pager: 204-931-954
Home Phone: 204-489-6924
Cell: 204-781-1502
Fax: 204-789-3926/204-489-6924


==============================Original Headers==============================
10, 21 -- From paul_hazelton-at-umanitoba.ca Sun Apr 2 20:24:51 2006
10, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23])
10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k331OovE014494;
10, 21 -- Sun, 2 Apr 2006 20:24:50 -0500
10, 21 -- Received: from [130.179.152.71] (cvx-008.cc.umanitoba.ca [130.179.152.71])
10, 21 -- (authenticated bits=0)
10, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id k331OkFm012539;
10, 21 -- Sun, 2 Apr 2006 20:24:48 -0500 (CDT)
10, 21 -- Message-ID: {4430795C.9060407-at-umanitoba.ca}
10, 21 -- Date: Sun, 02 Apr 2006 20:24:44 -0500
10, 21 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca}
10, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax)
10, 21 -- X-Accept-Language: en-us, en
10, 21 -- MIME-Version: 1.0
10, 21 -- To: zaluzec-at-microscopy.com
10, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com}
10, 21 -- Subject: Re: [Microscopy] Injector Blade?
10, 21 -- References: {200604021725.k32HP0bj028969-at-ns.microscopy.com}
10, 21 -- In-Reply-To: {200604021725.k32HP0bj028969-at-ns.microscopy.com}
10, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
10, 21 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Mon, 3 Apr 2006 07:36:35 -0500
Subject: [Microscopy] Re: Injector Blade?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Take a look at the Tips page on our web site (set out below) to see a
technique that we have used for many years to determine the TRUE cross
section of a fibre or similar material.

I do not believe that any cutting method, other than embedding and
sectioning, truly demonstrates the cross section of a material, fracturing
does!

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com

----- Original Message -----
X-from: {hinmeigeng-at-hotmail.com}
To: {protrain-at-emcourses.com}
Sent: Saturday, April 01, 2006 11:31 AM

Nestor,

An injector blade is something we bearded types have long happily
forgotten about. It's the narrow, single-edge razor blade that used
to go into injector-razors. The main competitor to double-edge razors
Way Back When. The "injector" bit was because of how they were
supplied: in a little metal carrier with a key that slid into the
back of the razor. The blade was then pushed into the razor
("injected"), simultaneously pushing out the old blade.
I used Schick Platinum-Plus injector blades in a Vibratome, they
worked much better than the commerical microscopy-supply house
microtome blades and were much cheaper.

Phil

} For general reference and because I'm just curious, what is an
} "injector blade" and when would you use it in a microtome
} instead of either diamond or glass blades.
}
} Or is this blade not used with microtomes?
}
}
}
} Nestor
} Your Friendly Neighborhood SysOp
--
Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
024C Brooks Hall
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
4, 22 -- From oshel1pe-at-cmich.edu Mon Apr 3 07:36:34 2006
4, 22 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k33CaYxB010877
4, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Apr 2006 07:36:34 -0500
4, 22 -- Received: from egateb.central.cmich.local ([141.209.15.85])
4, 22 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id k33DE14p031280
4, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Apr 2006 09:14:07 -0400
4, 22 -- Received: from [141.209.160.132] ([141.209.160.132]) by egateb.central.cmich.local with Microsoft SMTPSVC(5.0.2195.6713);
4, 22 -- Mon, 3 Apr 2006 08:36:08 -0400
4, 22 -- Mime-Version: 1.0
4, 22 -- Message-Id: {f06230903c056c6481aa4-at-[141.209.160.132]}
4, 22 -- In-Reply-To: {200604021728.k32HSSGX000601-at-ns.microscopy.com}
4, 22 -- References: {200604021728.k32HSSGX000601-at-ns.microscopy.com}
4, 22 -- Date: Mon, 3 Apr 2006 08:36:28 -0400
4, 22 -- To: Microscopy-at-microscopy.com
4, 22 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 22 -- Subject: Re: [Microscopy] Injector Blade?
4, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 22 -- X-OriginalArrivalTime: 03 Apr 2006 12:36:08.0741 (UTC) FILETIME=[2ECABD50:01C6571B]
4, 22 -- X-CanItPRO-Stream: default
4, 22 -- X-Spam-Score: -4 () L_EXCH_MF
4, 22 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21
==============================End of - Headers==============================




From: hagglundk1-at-nku.edu
Date: Mon, 3 Apr 2006 08:20:04 -0500
Subject: [Microscopy] Re: Replacement Argon Valve THANKS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to all who responded to my inquiry regarding the Replacement
valve. I was able to find several sources for adequate replacements.
The key was adding the term "metering valve" to my growing vocabulary.
With the proper name and an internet connection, it only took a few
minutes to find exactly what I needed.

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu


==============================Original Headers==============================
3, 23 -- From hagglundk1-at-nku.edu Mon Apr 3 08:20:04 2006
3, 23 -- Received: from mailfe2.nku.edu (mailfe2.nku.edu [192.122.237.68])
3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k33DK4Qs021072
3, 23 -- for {microscopy-at-microscopy.com} ; Mon, 3 Apr 2006 08:20:04 -0500
3, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211);
3, 23 -- Mon, 3 Apr 2006 09:20:02 -0400
3, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0
3, 23 -- Content-class: urn:content-classes:message
3, 23 -- MIME-Version: 1.0
3, 23 -- Content-Type: text/plain;
3, 23 -- charset="us-ascii"
3, 23 -- Subject: Re: [Microscopy] Replacement Argon Valve THANKS
3, 23 -- Date: Mon, 3 Apr 2006 09:20:01 -0400
3, 23 -- Message-ID: {F01E4499C4EC5842A8AB7198141AC72F3586D1-at-mailfac1.hh.nku.edu}
3, 23 -- X-MS-Has-Attach:
3, 23 -- X-MS-TNEF-Correlator:
3, 23 -- Thread-Topic: Re: [Microscopy] Replacement Argon Valve THANKS
3, 23 -- Thread-Index: AcZXIVBc5msXIo1MShuTFFZwXIUP9A==
3, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu}
3, 23 -- To: {microscopy-at-microscopy.com}
3, 23 -- X-OriginalArrivalTime: 03 Apr 2006 13:20:02.0668 (UTC) FILETIME=[50BC1AC0:01C65721]
3, 23 -- Content-Transfer-Encoding: 8bit
3, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k33DK4Qs021072
==============================End of - Headers==============================




From: jbs-at-temple.edu
Date: Mon, 3 Apr 2006 10:05:26 -0500
Subject: [Microscopy] Re: Injector Blade?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

For those of you who haven't been able to visualize this blade, Here
is a link to an old ad by Schick. I haven't been able to find a link
to the original AO blade holder that used these blades, but I do have
two in my lab that we are still using, both for paraffin and frozen
sections. We do get strange looks at the local pharmacy, though,
when we ask for the blades.


http://www.rareads.com/scans/8792.jpg

Joel


}
}
}
} ----------------------------------------------------------------------
} ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
}
} For general reference and because I'm just curious, what is an
} "injector blade" and when would you use it in a microtome
} instead of either diamond or glass blades.
}
} Or is this blade not used with microtomes?
}
}
}
} Nestor
} Your Friendly Neighborhood SysOp
}
} ==============================Original
} Headers============================== 6, 11 -- From
} zaluzec-at-microscopy.com Sun Apr 2 12:22:12 2006 6, 11 -- Received:
} from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 6, 11 -- by
} ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} k32HMBkP025186 6, 11 -- for {microscopy-at-microscopy.com} ; Sun, 2 Apr
} 2006 12:22:11 -0500 6, 11 -- Mime-Version: 1.0 6, 11 -- Message-Id:
} {p06110401c055b822c831-at-[206.69.208.22]} 6, 11 -- Date: Sun, 2 Apr 2006
} 12:22:10 -0500 6, 11 -- To: microscopy-at-microscopy.com 6, 11 -- From:
} "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 6, 11 -- Subject:
} Injector Blade? 6, 11 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of -
} Headers==============================


Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs


==============================Original Headers==============================
9, 27 -- From jbs-at-temple.edu Mon Apr 3 10:05:26 2006
9, 27 -- Received: from po-smtp3.temple.edu (po-smtp3.temple.edu [155.247.166.231])
9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k33F5PBh032118;
9, 27 -- Mon, 3 Apr 2006 10:05:26 -0500
9, 27 -- Received: from [155.247.98.40] (jbs.bio.temple.edu [155.247.98.40])
9, 27 -- by po-smtp3.temple.edu (MOS 3.7.1-GA)
9, 27 -- with ESMTP id CLY03759 (AUTH jbs);
9, 27 -- Mon, 3 Apr 2006 11:05:23 -0400 (EDT)
9, 27 -- From: "Joel Sheffield" {jbs-at-temple.edu}
9, 27 -- To: zaluzec-at-microscopy.com, microscopy-at-microscopy.com
9, 27 -- Date: Mon, 03 Apr 2006 11:03:31 -0400
9, 27 -- MIME-Version: 1.0
9, 27 -- Subject: Re: [Microscopy] Injector Blade?
9, 27 -- Reply-to: jbs-at-temple.edu
9, 27 -- Message-ID: {44310103.18434.47483273-at-jbs.temple.edu}
9, 27 -- Priority: normal
9, 27 -- In-reply-to: {200604021722.k32HMRhS025399-at-ns.microscopy.com}
9, 27 -- X-mailer: Pegasus Mail for Windows (4.30 public beta 1)
9, 27 -- Content-type: text/plain; charset=US-ASCII
9, 27 -- Content-transfer-encoding: 7BIT
9, 27 -- Content-description: Mail message body
9, 27 -- X-Junkmail-Status: score=10/50, host=po-smtp3.temple.edu
9, 27 -- X-Junkmail-SD-Raw: score=unknown,
9, 27 -- refid=str=0001.0A090206.443138C4.0024,ss=1,fgs=0,
9, 27 -- ip=155.247.98.40,
9, 27 -- so=2005-09-30 22:39:37,
9, 27 -- dmn=5.1.5/2006-02-08
==============================End of - Headers==============================




From: milesd-at-us.ibm.com
Date: Mon, 3 Apr 2006 11:07:59 -0500
Subject: [Microscopy] Injector Blade?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

A little off topic repose, if you don't mind :o)

In my younger days, when the 2 blade razors were being advertised, I was
watching "Saturday Night Live". They would do some spoof ads, and they
would do a pretty good job of it. You could hardly tell the difference
between their ads, and the real ones. They put together a great ad for a 3
bladed razor that looked pretty real, compared to the 2 blade razor ads.
At the end of the ad, they ended with the line, "Because you'll believe
anything!" Years later, I saw a real ad for the triple edged razor, and
that old "Saturday Night Live" ad came back to me. Now they are selling 5
edged razors! They must work well, because they are selling. I wish I had
patented the idea all those years ago...

Have a good day (or evening)!

Darrell

paul_hazelton-at-umanitoba.ca wrote on 04/02/2006 09:26:13 PM:

}
}
}
}
----------------------------------------------------------------------------

} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------

}
} Ah yes, how fondly i remember my old Schick injector. First razor -
} took it to university with me. Then they got chromium edged blades,
} Then someone decided we needed two blades, and the rest was history.
} Now we can get 5 blades, with a motor of some sort, too. In spite of
} the nostalgia, must confess that it did cut my face up all to heck and
} gone. But i still have the old razor....
}
}
} While I'm tired of cutting my face up, I still use injector blades in
} the blade holder for my old ultratome III to trim blocks.
}
}
} paul
}
}
}
}
} Paul R. Hazelton, PhD
} Electron Microscope Unit
} University of Manitoba
} Department of Medical Microbiology
} 531 Basic Medical Sciences Building
} 730 William Avenue
} Winnipeg, Manitoba, Canada, R3E 0W3
} e-mail: paul_hazelton-at-umanitoba.ca
} Work Phone: 204-789-3313
} Pager: 204-931-954
} Home Phone: 204-489-6924
} Cell: 204-781-1502
} Fax: 204-789-3926/204-489-6924
}
}
} ==============================Original
Headers==============================
} 10, 21 -- From paul_hazelton-at-umanitoba.ca Sun Apr 2 20:24:51 2006
} 10, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.
} umanitoba.ca [130.179.16.23])
} 10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
} ESMTP id k331OovE014494;
} 10, 21 -- Sun, 2 Apr 2006 20:24:50 -0500
} 10, 21 -- Received: from [130.179.152.71] (cvx-008.cc.umanitoba.ca
} [130.179.152.71])
} 10, 21 -- (authenticated bits=0)
} 10, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP
} id k331OkFm012539;
} 10, 21 -- Sun, 2 Apr 2006 20:24:48 -0500 (CDT)
} 10, 21 -- Message-ID: {4430795C.9060407-at-umanitoba.ca}
} 10, 21 -- Date: Sun, 02 Apr 2006 20:24:44 -0500
} 10, 21 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca}
} 10, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-
} US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax)
} 10, 21 -- X-Accept-Language: en-us, en
} 10, 21 -- MIME-Version: 1.0
} 10, 21 -- To: zaluzec-at-microscopy.com
} 10, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com}
} 10, 21 -- Subject: Re: [Microscopy] Injector Blade?
} 10, 21 -- References: {200604021725.k32HP0bj028969-at-ns.microscopy.com}
} 10, 21 -- In-Reply-To: {200604021725.k32HP0bj028969-at-ns.microscopy.com}
} 10, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} 10, 21 -- Content-Transfer-Encoding: 7bit
} ==============================End of -
Headers==============================


==============================Original Headers==============================
9, 27 -- From milesd-at-us.ibm.com Mon Apr 3 11:07:58 2006
9, 27 -- Received: from e3.ny.us.ibm.com (e3.ny.us.ibm.com [32.97.182.143])
9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k33G7wbU010192
9, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 3 Apr 2006 11:07:58 -0500
9, 27 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234])
9, 27 -- by e3.ny.us.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id k33G7lZx008773
9, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 3 Apr 2006 12:07:47 -0400
9, 27 -- Received: from d01av04.pok.ibm.com (d01av04.pok.ibm.com [9.56.224.64])
9, 27 -- by d01relay02.pok.ibm.com (8.12.10/NCO/VER6.8) with ESMTP id k33G7arN249918
9, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 3 Apr 2006 12:07:37 -0400
9, 27 -- Received: from d01av04.pok.ibm.com (loopback [127.0.0.1])
9, 27 -- by d01av04.pok.ibm.com (8.12.11/8.13.3) with ESMTP id k33G7arZ012435
9, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 3 Apr 2006 12:07:36 -0400
9, 27 -- Received: from d01ml076.pok.ibm.com (d01ml076.pok.ibm.com [9.56.229.50])
9, 27 -- by d01av04.pok.ibm.com (8.12.11/8.12.11) with ESMTP id k33G7a3T012414
9, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 3 Apr 2006 12:07:36 -0400
9, 27 -- In-Reply-To: {200604030126.k331QDaD015919-at-ns.microscopy.com}
9, 27 -- Subject: Re: [Microscopy] Re: Injector Blade?
9, 27 -- To: Microscopy-at-MSA.Microscopy.Com
9, 27 -- X-Mailer: Lotus Notes Release 7.0 HF85 November 04, 2005
9, 27 -- Message-ID: {OFF56D0893.E91E22F3-ON85257145.0054402D-85257145.0058956C-at-us.ibm.com}
9, 27 -- From: Darrell Miles {milesd-at-us.ibm.com}
9, 27 -- Date: Mon, 3 Apr 2006 12:07:34 -0400
9, 27 -- X-MIMETrack: Serialize by Router on D01ML076/01/M/IBM(Release 6.5.4FP3 HF3|February 22, 2006) at
9, 27 -- 04/03/2006 12:07:35
9, 27 -- MIME-Version: 1.0
9, 27 -- Content-type: text/plain; charset=US-ASCII
==============================End of - Headers==============================




From: gvrdolja-at-nature.berkeley.edu
Date: Mon, 3 Apr 2006 12:12:13 -0500
Subject: [Microscopy] question about infinite focus stereo microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
I meant to look this up over the weekend, but my internet and phone was
down. I thought I would ask this here first.

I wanted to find papers or references on the infinite focus types of
stereo microscopes that combine in-focus regions of a series of images
into one image. A prodoct that is commercially available that is similar
is from alicona imaging called an infinite focus microscope.

Can anyone forward me references or journal references? Any guidance
greatly appreciated.
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

==============================Original Headers==============================
4, 24 -- From gvrdolja-at-nature.berkeley.edu Mon Apr 3 12:12:12 2006
4, 24 -- Received: from nature.Berkeley.EDU (nature.Berkeley.EDU [128.32.253.219])
4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k33HCBSh020695
4, 24 -- for {microscopy-at-microscopy.com} ; Mon, 3 Apr 2006 12:12:12 -0500
4, 24 -- Received: from localhost (localhost [127.0.0.1])
4, 24 -- by nature.Berkeley.EDU (Postfix) with ESMTP id 18394C1E70
4, 24 -- for {microscopy-at-microscopy.com} ; Mon, 3 Apr 2006 10:12:11 -0700 (PDT)
4, 24 -- Received: from nature.Berkeley.EDU ([127.0.0.1])
4, 24 -- by localhost (nature.berkeley.edu [127.0.0.1]) (amavisd-new, port 10024)
4, 24 -- with ESMTP id 15229-04-2 for {microscopy-at-microscopy.com} ;
4, 24 -- Mon, 3 Apr 2006 10:12:10 -0700 (PDT)
4, 24 -- Received: by nature.Berkeley.EDU (Postfix, from userid 7458)
4, 24 -- id A9935C1E5C; Mon, 3 Apr 2006 10:12:10 -0700 (PDT)
4, 24 -- Received: from localhost (localhost [127.0.0.1])
4, 24 -- by nature.Berkeley.EDU (Postfix) with ESMTP id 94CB7C1E57
4, 24 -- for {microscopy-at-microscopy.com} ; Mon, 3 Apr 2006 10:12:10 -0700 (PDT)
4, 24 -- Date: Mon, 3 Apr 2006 10:12:10 -0700 (PDT)
4, 24 -- From: Gordon Vrololjak {gvrdolja-at-nature.berkeley.edu}
4, 24 -- To: microscopy-at-microscopy.com
4, 24 -- Subject: question about infinite focus stereo microscopes
4, 24 -- Message-ID: {Pine.SOC.4.64.0604031008050.2468-at-nature.Berkeley.EDU}
4, 24 -- MIME-Version: 1.0
4, 24 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
4, 24 -- X-Virus-Scanned: amavisd-new at nature.berkeley.edu
==============================End of - Headers==============================




From: scanning-at-fams.org
Date: Mon, 3 Apr 2006 12:33:17 -0500
Subject: [Microscopy] SCANNING 2006 Final Program Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

The Final Program for SCANNING 2006 is now available at
www.scanning.org. The Registration Form is also available online and we
encourage you to register today to ensure your first choice for Short
Courses and Sessions.

We look forward to seeing you April 25-27 at the Hotel Washington in
Washington, D.C.

Best regards,


Phaedra McGuinness
Managing Editor
SCANNING, The Journal of Scanning Microscopies
P.O. Box 485
Mahwah, NJ 07430
Tel: (201) 818-1010 * Fax: (201) 818-0086 *email: scanning-at-fams.org *
Web: www.scanning.org


==============================Original Headers==============================
7, 19 -- From scanning-at-fams.org Mon Apr 3 12:33:17 2006
7, 19 -- Received: from smtp-out2.oct.nac.net (smtp-out2.oct.nac.net [209.123.233.212])
7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k33HXHtC030476
7, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 3 Apr 2006 12:33:17 -0500
7, 19 -- Received: (qmail 5277 invoked from network); 3 Apr 2006 13:33:14 -0400
7, 19 -- Received: from unknown (HELO mail1.oct.nac.net) (209.123.233.241)
7, 19 -- by smtp-out2.oct.nac.net with SMTP; 3 Apr 2006 13:33:14 -0400
7, 19 -- Received: (qmail 11080 invoked from network); 3 Apr 2006 13:33:13 -0400
7, 19 -- Received: from unknown (HELO ?192.168.2.80?) (64.21.7.106)
7, 19 -- by mail1.oct.nac.net with SMTP; 3 Apr 2006 13:33:13 -0400
7, 19 -- Mime-Version: 1.0 (Apple Message framework v623)
7, 19 -- Content-Transfer-Encoding: 7bit
7, 19 -- Message-Id: {5df5038113c67c6e148cc4f3c6328d07-at-fams.org}
7, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
7, 19 -- To: Microscopy List Server Server {Microscopy-at-MSA.Microscopy.Com}
7, 19 -- From: Phaedra McGuinness {scanning-at-fams.org}
7, 19 -- Subject: SCANNING 2006 Final Program Available
7, 19 -- Date: Mon, 3 Apr 2006 13:33:13 -0400
7, 19 -- X-Mailer: Apple Mail (2.623)
==============================End of - Headers==============================




From: andrewb-at-vsl.cua.edu
Date: Mon, 3 Apr 2006 13:01:37 -0500
Subject: [Microscopy] Injector Blade?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The blades are still available through microscopy supply houses. I
use them for many purposes myself. Maybe someone already
pointed this out, but I thought I'd mention it since it sounds like some
people are still looking for a source. There is even a picture of the
dispenser on the Ted Pella site/catalog.

On 3 Apr 2006 at 10:12, jbs-at-temple.edu wrote:

} For those of you who haven't been able to visualize this blade, Here
} is a link to an old ad by Schick. I haven't been able to find a link
} to the original AO blade holder that used these blades, but I do have
} two in my lab that we are still using, both for paraffin and frozen
} sections. We do get strange looks at the local pharmacy, though,
} when we ask for the blades.
}
}
} http://www.rareads.com/scans/8792.jpg
}
} Joel

All the best,

Andy Buechele, Washington, D.C., U.S.A.


==============================Original Headers==============================
6, 22 -- From andrewb-at-mail.vsl.cua.edu Mon Apr 3 13:01:36 2006
6, 22 -- Received: from hermes.vsl.cua.edu (interface.vsl.cua.edu [136.242.188.2])
6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k33I1XEC007894
6, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Apr 2006 13:01:34 -0500
6, 22 -- X-Filtered-With-Copfilter: Version 0.82 (ProxSMTP 1.3.91)
6, 22 -- X-Copfilter-Virus-Scanned: ClamAV 0.88/1370 - Mon Apr 3 12:31:59 2006
6, 22 -- X-Copfilter: Client is part of our network, skipped SpamAssassin
6, 22 -- Received: from andrewb_409 [136.242.189.249] by hermes.vsl.cua.edu with ESMTP
6, 22 -- (SMTPD-8.21) id A2FC0350; Mon, 03 Apr 2006 14:01:32 -0400
6, 22 -- From: "Andrew Buechele" {andrewb-at-vsl.cua.edu}
6, 22 -- To: Microscopy-at-microscopy.com
6, 22 -- Date: Mon, 03 Apr 2006 14:01:32 -0400
6, 22 -- MIME-Version: 1.0
6, 22 -- Subject: Re: [Microscopy] Re: Injector Blade?
6, 22 -- Reply-to: andrewb-at-vsl.cua.edu
6, 22 -- Message-ID: {44312ABC.32465.75EBF0-at-localhost}
6, 22 -- Priority: normal
6, 22 -- In-reply-to: {200604031512.k33FCsmB008434-at-ns.microscopy.com}
6, 22 -- X-mailer: Pegasus Mail for Windows (v4.02a)
6, 22 -- Content-type: text/plain; charset=US-ASCII
6, 22 -- Content-transfer-encoding: 7BIT
6, 22 -- Content-description: Mail message body
==============================End of - Headers==============================




From: chao.wang-at-materials.ox.ac.uk
Date: Mon, 3 Apr 2006 21:11:17 -0500
Subject: [Microscopy] viaWWW: help for MgO prepration and Fe oxidation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both chao.wang-at-materials.ox.ac.uk as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: chao.wang-at-materials.ox.ac.uk
Name: Chao Wang

Organization: Oxford Materials

Title-Subject: [Filtered] help for MgO prepration and Fe oxidation

Question: Dear All

I have three problems, could you tell me some suggestions:

1.single crystale MgO preparation

I need very good quality cross section TEM sample. I now manully polish it to 70 micron and dimple to abount 20 micron and then ion milling (PIPS). But it's not so good. The edge looks sharp and MgO cracks a lot when I ground lower then 60 micron on diamond papers. Do you have better ideas to get very thin specimen?

2. Fe oxidiation
Another problem is Fe oxidation (on top of MgO substrate), it seems after some time, the sample oxidized a lot, even I got very thin specimen, it looks like amorphous (it should be crystalline). How to keep it? (I use plasma cleaning, not working very well)

3. Beam sensitive to MgO sample

Sometime the beam in TEM is sensitive to MgO sample, how to get rid of this?

Your help is very appreicated!

best wishes

Chao

---------------------------------------------------------------------------

==============================Original Headers==============================
15, 12 -- From zaluzec-at-microscopy.com Mon Apr 3 21:11:17 2006
15, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
15, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k342BGYK026336
15, 12 -- for {microscopy-at-microscopy.com} ; Mon, 3 Apr 2006 21:11:17 -0500
15, 12 -- Mime-Version: 1.0
15, 12 -- X-Sender: (Unverified)
15, 12 -- Message-Id: {p06110402c057863616fc-at-[206.69.208.22]}
15, 12 -- Date: Mon, 3 Apr 2006 21:11:15 -0500
15, 12 -- To: microscopy-at-microscopy.com
15, 12 -- From: chao.wang-at-materials.ox.ac.uk (by way of MicroscopyListserver)
15, 12 -- Subject: viaWWW: help for MgO prepration and Fe oxidation
15, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: rra-at-stowers-institute.org
Date: Mon, 3 Apr 2006 21:12:03 -0500
Subject: [Microscopy] viaWWW: Leica EM-Stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rra-at-stowers-institute.org as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: rra-at-stowers-institute.org
Name: Rhonda Allen

Organization: Stowers Institute

Title-Subject: [Filtered] Leica EM-Stain

Question: Hello, I am interested in purchasing the Leica EM-Stain. Does anyone have any comments, positive and negative, that I should take into consideration before making such an investment? We will be using it on formvar coated slot grids. Thanks in advance, Rhonda Allen BA HT(ASCP)HTL, QIHC Stowers Institute for Medical Research Kansas City, Missouri 816-926-4346


---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Mon Apr 3 21:12:03 2006
7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k342C2tI026972
7, 12 -- for {microscopy-at-microscopy.com} ; Mon, 3 Apr 2006 21:12:02 -0500
7, 12 -- Mime-Version: 1.0
7, 12 -- X-Sender: (Unverified)
7, 12 -- Message-Id: {p06110403c0578663217d-at-[206.69.208.22]}
7, 12 -- Date: Mon, 3 Apr 2006 21:12:01 -0500
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: rra-at-stowers-institute.org (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: Leica EM-Stain
7, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: drm-at-ansto.gov.au
Date: Mon, 3 Apr 2006 23:52:46 -0500
Subject: [Microscopy] help for MgO prepration and Fe oxidati

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Chao Wang

I too have experienced similar problems in
preparing thin specimens from single crystal MgO.
I have had some success with plan view specimens
ie ion milling away the substrate to perforate
through into a thin oxide film grown on it (See
J. Cryst. Growth 285 (2005) p208-214). Here
samples were ground to about 80µms thick, then
dimpled. I never get too adventurous with
dimpling - aiming for a thickness of 30µms. Any
thinner and the MgO has a strong inclination to
fracture. All grinding and dimpling is done very
gently to avoid cracking the substrate. I then
ion mill in a PIPs at 5keV, 6 deg, until the
sample is near perforation, then reduce the angle
to 4 deg and the voltage to 3keV and then mill to
electron transparency. To liberate the specimen
from the mounting post I soak it in acetone till
it drops off rather than heating to soften the
crystal bond and slide it off - the latter is
guaranteed to break thin regions off. Tripod
Polishing was of no use at all, aside from the
coarse grinding step to get down to about 80µms.

Success was very hit and miss. I suspect my MgO
crystals had a high degrees of residual stress.
Cross sectioning is even harder and requires a
lot of patience to get any kind of result.
Specimens just disintegrate in the PIPS without
any mechanical handling. Focused ion beam milling
may help if you have access to one.

With regard to oxidation of Fe. My experience is
with electropolished foils rather than thin
films, but there are some similarities. Storage
in solvents like methanol is definitely not
recommended - such polar solvents are highly
corrosive to iron. Storage in a non-polar solvent
like a hydrocarbon may stop oxidation, but
cleaning it up for TEM examination could be
challenge without a plasma cleaner. Storage in
vacuum is surprisingly bad for electropolished
foils and they oxidise much more severely than
storage in a normal lab desiccator. I suspect the
protective hydrated film formed from
electropolishing dries out in vacuum and cracks
allowing oxidation to proceed. Of course your
films don't have such a layer, so vacuum storage
may be no worse or better than a good lab
desiccator. Probably the key factor governing
oxidation is the level of water vapour your
specimen gets exposed to.

With respect to beam sensitivity - I presume you
mean charging rather than beam damage? At 200keV
I did not experience significant damage in the
MgO substrate, but electron beam charging was a
major issue. I therefore always evaporate a very
thin layer of carbon (20Å) onto MgO specimens
prior to TEM examination. If the film grown on
the MgO is not very conductive, then I coat both
sides. In your case you have a conductive layer
on one side (Fe), so you may get away with
coating just the MgO side.

I hope this helps.

Regards

Dave Mitchell
--
Dr David Mitchell
ANSTO Materials
PMB 1
Menai
NSW 2234
Australia

tel 61 2 9717 3456
fax 61 2 9543 7179



==============================Original Headers==============================
11, 25 -- From drm-at-ansto.gov.au Mon Apr 3 23:52:41 2006
11, 25 -- Received: from tachyon.gw.ansto.gov.au (anstogw.gw.ansto.gov.au [137.157.8.253])
11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k344qejx015539
11, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Apr 2006 23:52:41 -0500
11, 25 -- Received: (from uucp-at-localhost)
11, 25 -- by tachyon.gw.ansto.gov.au (8.11.7p1+Sun/8.11.7) id k344qcC15261
11, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 14:52:38 +1000 (EST)
11, 25 -- Received: from jenner.ansto.gov.au(137.157.59.25) by tachyon.gw.ansto.gov.au via csmap (V6.0)
11, 25 -- id srcAAARlaGZD; Tue, 4 Apr 06 14:52:38 +1000
11, 25 -- Received: from hadron.ansto.gov.au (hadron.ansto.gov.au [137.157.13.219])
11, 25 -- by jenner.ansto.gov.au (8.12.10/8.12.10) with ESMTP id k344qXk2006413
11, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 14:52:33 +1000
11, 25 -- Received: from [137.157.94.26] (m0374m.ansto.gov.au [137.157.94.26])
11, 25 -- by hadron.ansto.gov.au (8.9.3/8.9.3) with ESMTP id OAA06227
11, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 14:52:31 +1000 (EST)
11, 25 -- Mime-Version: 1.0
11, 25 -- Message-Id: {a06230914c057a30b5d12-at-[137.157.94.26]}
11, 25 -- Date: Tue, 4 Apr 2006 14:52:37 +1000
11, 25 -- To: Microscopy-at-microscopy.com
11, 25 -- From: David Mitchell {drm-at-ansto.gov.au}
11, 25 -- Subject: help for MgO prepration and Fe oxidati
11, 25 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
11, 25 -- X-ANSTO-MailScanner: Found to be clean
11, 25 -- Content-Transfer-Encoding: 8bit
11, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k344qejx015539
==============================End of - Headers==============================




From: Colin.Veitch-at-csiro.au
Date: Tue, 4 Apr 2006 00:20:31 -0500
Subject: [Microscopy] Problem with a Philips EM410 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I have a Philips 410 TEM which has decided not to cooperate! I am not
overly familiar with this machine so I apologise if I have missed
something obvious.

When you turn on the system and begin the pumpdown sequence the rotary
pump starts and after around 30 seconds (the vacuum gauge drops to
around 10 on the scale) the rotary pump shuts off and that is it.

The vacuum system indicators (valve status) on the side panel all remain
off. I have checked all the fuses and they are OK. There is plenty of
cooling water (going in and coming out). The diffusion pump heater
seems to be OK (it is not open circuit, nor ohms resistance). The
pneumatic gas pressure is also OK.

Any help as to a possible cause/cure would be appreciated.

Cheers.

Colin Veitch

Electron Microscopist

CSIRO Textile and Fibre Technology

PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au

Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mob: 0438 538 475
Fax: +61 (0) 3 5246 4811



The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may
not copy, distribute or take any action in reliance on it. If you have
received this message in error, please telephone CSIRO Textile and Fibre
Technology on +61 3 5246 4000.



==============================Original Headers==============================
17, 30 -- From Colin.Veitch-at-csiro.au Tue Apr 4 00:20:22 2006
17, 30 -- Received: from vic-MTAout2.csiro.au (vic-MTAout2.csiro.au [150.229.64.38])
17, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k345KLVZ025429
17, 30 -- for {microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 00:20:21 -0500
17, 30 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=dBF7dfKXSlt81lkUABrPqUbsCIwjoURW1HJ9hzb0jZaxVI1s/fKCKoicAEX1Lot9kXcu1OaMU4mhpGKGJD9rj0Yt5OO80G/XIVKO1w6MjYmSjaEgTZ03epxNTfKPHGmp;
17, 30 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56])
17, 30 -- by vic-MTAout2.csiro.au with ESMTP; 04 Apr 2006 15:20:20 +1000
17, 30 -- X-IronPort-AV: i="4.03,160,1141563600";
17, 30 -- d="scan'208"; a="73879638:sNHT25511800"
17, 30 -- Received: from exvicn1-mel.nexus.csiro.au ([138.194.3.60]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713);
17, 30 -- Tue, 4 Apr 2006 15:20:19 +1000
17, 30 -- Received: from exvic5-gex.nexus.csiro.au ([138.194.194.6]) by exvicn1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713);
17, 30 -- Tue, 4 Apr 2006 15:20:19 +1000
17, 30 -- X-MIMEOLE: Produced By Microsoft Exchange V6.0.6603.0
17, 30 -- Content-Class: urn:content-classes:message
17, 30 -- MIME-Version: 1.0
17, 30 -- Content-Type: text/plain;
17, 30 -- charset="us-ascii"
17, 30 -- Subject: Problem with a Philips EM410 TEM
17, 30 -- Date: Tue, 4 Apr 2006 15:20:18 +1000
17, 30 -- Message-ID: {32CDDDAA7161394599F0025494915749024813-at-exvic5-gex.nexus.csiro.au}
17, 30 -- X-MS-Has-Attach:
17, 30 -- X-MS-TNEF-Correlator:
17, 30 -- Thread-Topic: Problem with a Philips EM410 TEM
17, 30 -- Thread-Index: AcZXp3YO7P7oXMhtRkmVma7B0e0d9A==
17, 30 -- From: {Colin.Veitch-at-csiro.au}
17, 30 -- To: {microscopy-at-microscopy.com}
17, 30 -- X-OriginalArrivalTime: 04 Apr 2006 05:20:19.0708 (UTC) FILETIME=[772AB3C0:01C657A7]
17, 30 -- Content-Transfer-Encoding: 8bit
17, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k345KLVZ025429
==============================End of - Headers==============================




From: opto-at-klughammer.de
Date: Tue, 4 Apr 2006 04:05:20 -0500
Subject: [Microscopy] Re: question about infinite focus stereo microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

please contact me offline.
I can send you information on how you can combine a stack of
stereomicroscope images and get a result image with "infinite" focus.

Regards

Anneliese Schmaus
Product Manager

klughammer bio gmbh
Strassbach 9
85229 Markt Indersdorf
Germany
Tel. +49 08136 6011
Fax +49 08136 7098
info-at-klughammer.de
www.klughammer.de

gvrdolja-at-nature.berkeley.edu schrieb:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America




==============================Original Headers==============================
10, 20 -- From opto-at-klughammer.de Tue Apr 4 04:05:20 2006
10, 20 -- Received: from moutng.kundenserver.de (moutng.kundenserver.de [212.227.126.171])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3495JqT005991
10, 20 -- for {microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 04:05:19 -0500
10, 20 -- Received: from [84.151.123.26] (helo=[192.168.2.101])
10, 20 -- by mrelayeu.kundenserver.de (node=mrelayeu10) with ESMTP (Nemesis),
10, 20 -- id 0ML31I-1FQhTS0Qsq-0003Zo; Tue, 04 Apr 2006 11:05:18 +0200
10, 20 -- Message-ID: {443236CB.8080305-at-klughammer.de}
10, 20 -- Date: Tue, 04 Apr 2006 11:05:15 +0200
10, 20 -- From: Klughammer GmbH {opto-at-klughammer.de}
10, 20 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716)
10, 20 -- X-Accept-Language: de-DE, de, en-us, en
10, 20 -- MIME-Version: 1.0
10, 20 -- To: microscopy-at-microscopy.com
10, 20 -- Subject: Re: [Microscopy] question about infinite focus stereo microscopes
10, 20 -- References: {200604031714.k33HEwk4024634-at-ns.microscopy.com}
10, 20 -- In-Reply-To: {200604031714.k33HEwk4024634-at-ns.microscopy.com}
10, 20 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed
10, 20 -- Content-Transfer-Encoding: 7bit
10, 20 -- X-Provags-ID: kundenserver.de abuse-at-kundenserver.de login:0ff4ff792c9129f92cf390aadeab6682
==============================End of - Headers==============================




From: christopher.hayden-at-novartis.com
Date: Tue, 4 Apr 2006 06:11:07 -0500
Subject: [Microscopy] Re: viaWWW: Leica EM-Stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Good Morning, Rhonda:

We have a Leica EM-Stain. We purchased it about a year ago in a
quest to make our staining absolutely consistent, and in regard to that,
it works wonderfully. Given all of the options for wetting times, stain
times, stain temperatures, etc, it's pretty versatile and dead-on
accurate, not to mention it frees up a lot of a technician's time!
However, we're had nothing but problems with the unit. We've gone
though 3 pumps already, numerous service calls, etc. In Leica's defense,
they may have had a bad batch of stain (high amounts of precipitate),
which is clogging up the pump and the filters. Speaking of the stain,
you're "required" to use their pre-made stains, else you void the warranty
on the unit. There's a bit of upkeep with the stainer as well, in that you
must perform a cleaning cycle at least once a week (which only takes a few
minutes to get running but someone still has to remember to do it), and it
uses 3% Nitric acid for the cleaning cycle, which possibly adds another
waste stream for your lab.
All in all, it would be a great unit if there weren't so many
problems with it. We may just have ended up with a lemon, though. Hard to
say.

-Chris






rra-at-stowers-institute.org
04/03/2006 10:20 PM
Please respond to rra


To: Christopher Hayden/PH/Novartis-at-PH
cc:
Subject: [Microscopy] viaWWW: Leica EM-Stain





----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both rra-at-stowers-institute.org as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: rra-at-stowers-institute.org
Name: Rhonda Allen

Organization: Stowers Institute

Title-Subject: [Filtered] Leica EM-Stain

Question: Hello, I am interested in purchasing the Leica EM-Stain. Does
anyone have any comments, positive and negative, that I should take into
consideration before making such an investment? We will be using it on
formvar coated slot grids. Thanks in advance, Rhonda Allen BA HT(ASCP)HTL,
QIHC Stowers Institute for Medical Research Kansas City, Missouri
816-926-4346


---------------------------------------------------------------------------

==============================Original
Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Mon Apr 3 21:12:03 2006
7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com
[206.69.208.22])
7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
with ESMTP id k342C2tI026972
7, 12 -- for {microscopy-at-microscopy.com} ; Mon, 3 Apr 2006
21:12:02 -0500
7, 12 -- Mime-Version: 1.0
7, 12 -- X-Sender: (Unverified)
7, 12 -- Message-Id: {p06110403c0578663217d-at-[206.69.208.22]}
7, 12 -- Date: Mon, 3 Apr 2006 21:12:01 -0500
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: rra-at-stowers-institute.org (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: Leica EM-Stain
7, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of -
Headers==============================




==============================Original Headers==============================
25, 30 -- From christopher.hayden-at-novartis.com Tue Apr 4 06:11:07 2006
25, 30 -- Received: from mail84.messagelabs.com (mail84.messagelabs.com [195.245.231.99])
25, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k34BB6Kf017329
25, 30 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 4 Apr 2006 06:11:06 -0500
25, 30 -- X-VirusChecked: Checked
25, 30 -- X-Env-Sender: christopher.hayden-at-novartis.com
25, 30 -- X-Msg-Ref: server-4.tower-84.messagelabs.com!1144149064!19910770!1
25, 30 -- X-StarScan-Version: 5.5.9.1; banners=-,-,-
25, 30 -- X-Originating-IP: [160.62.1.174]
25, 30 -- Received: (qmail 13192 invoked from network); 4 Apr 2006 11:11:05 -0000
25, 30 -- Received: from ch2ssaenov01.novartis.com (HELO ch2ssaenov01.novartis.com) (160.62.1.174)
25, 30 -- by server-4.tower-84.messagelabs.com with AES256-SHA encrypted SMTP; 4 Apr 2006 11:11:05 -0000
25, 30 -- Received: from mtap2.is.chbs ([192.37.33.19])
25, 30 -- by ch2ssaenov01.novartis.com (8.13.6/8.13.4) with ESMTP id k34B8vkO027268;
25, 30 -- Tue, 4 Apr 2006 13:08:57 +0200
25, 30 -- Received: from phchbs-s3025.EU.novartis.net (phchbs-s3025.eu.novartis.net [192.37.31.249])
25, 30 -- by mtap2.is.chbs (Switch-3.1.6/Switch-3.1.6) with ESMTP id k34BB3nH9486452;
25, 30 -- Tue, 4 Apr 2006 13:11:04 +0200
25, 30 -- To: Microscopy-at-msa.microscopy.com
25, 30 -- Cc: rra-at-stowers-institute.org
25, 30 -- Subject: Re: [Microscopy] viaWWW: Leica EM-Stain
25, 30 -- MIME-Version: 1.0
25, 30 -- X-Mailer: Lotus Notes Release 5.0.12 February 13, 2003
25, 30 -- Message-ID: {OF829E7CC2.942F7902-ON85257146.003C549D-85257146.003D705C-at-EU.novartis.net}
25, 30 -- From: christopher.hayden-at-novartis.com
25, 30 -- Date: Tue, 4 Apr 2006 07:11:20 -0400
25, 30 -- X-MIMETrack: Serialize by Router on CHBSSPH0/PH/Novartis(5012HF433 | October 14, 2003) at
25, 30 -- 04.04.2006 13:11:04,
25, 30 -- Serialize complete at 04.04.2006 13:11:04
25, 30 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Tue, 4 Apr 2006 07:14:43 -0500
Subject: [Microscopy] Re: Problem with a Philips EM410 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Colin,

I don't know the Philips 410, so this may be useless, but I did have
a similar problem with a JEOL SEM, and the cure I think may be
generic.
The issue is that if a given vacuum, as registered on the vacuum
control board as a given current (e.g. {50mA) or voltage (e.g.??), is
not shown, the system doesn't switch to the diff pump, but shuts
down. The cure was to go to manual pumping and allow the 'scope to
pump with the rotary pump for longer than the electronics would allow
(usually 2 to 5 minutes). Then manually go to high vacuum and let run
for a day or two. It should then cycle OK.
This was after the 'scope had been sitting with a static vacuum for a
few weeks.
There should be a test point on the vacuum board where the required
current (voltage) can be read, and this should be in the manual
somewhere. Servicing the vacuum system or the like, maybe
troubleshooting.
The other thought is, it's after April Fool's day, do you have any
pranksters in the department? In a previous incarnation, I had a
service engineer for a now-defunct EM company try to sell me a new
diff pump (for $12,000) by placing a nickel between the heater and
the pump bottom. The pump didn't heat as well, obviously, so the
vacuum was degraded, and there was a cheery red glow from the bottom
of the pump. (No, they didn't sell a pump.)

Phil
Now I know Oz is different -- you give your mobs numbers, and we give
our gangs names.

} Hi,
}
} I have a Philips 410 TEM which has decided not to cooperate! I am not
} overly familiar with this machine so I apologise if I have missed
} something obvious.
}
} When you turn on the system and begin the pumpdown sequence the rotary
} pump starts and after around 30 seconds (the vacuum gauge drops to
} around 10 on the scale) the rotary pump shuts off and that is it.
}
} The vacuum system indicators (valve status) on the side panel all remain
} off. I have checked all the fuses and they are OK. There is plenty of
} cooling water (going in and coming out). The diffusion pump heater
} seems to be OK (it is not open circuit, nor ohms resistance). The
} pneumatic gas pressure is also OK.
}
} Any help as to a possible cause/cure would be appreciated.
}
} Cheers.
}
} Colin Veitch
} Electron Microscopist
} CSIRO Textile and Fibre Technology
} PO Box 21, BELMONT, Vic. 3216. Australia.
} E-mail: colin.veitch-at-csiro.au
} Web: http://www.tft.csiro.au
} Tel: +61 (0) 3 5246 4000
} Mob: 0438 538 475
} Fax: +61 (0) 3 5246 4811
}
} The information contained in this e-mail message may be privileged or
} confidential information. If you are not an intended recipient, you may
} not copy, distribute or take any action in reliance on it. If you have
} received this message in error, please telephone CSIRO Textile and Fibre
} Technology on +61 3 5246 4000.
--
Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
024C Brooks Hall
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
4, 23 -- From oshel1pe-at-cmich.edu Tue Apr 4 07:14:43 2006
4, 23 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21])
4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k34CEho9027735
4, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 07:14:43 -0500
4, 23 -- Received: from egateb.central.cmich.local ([141.209.15.85])
4, 23 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id k34Cpx4p006142;
4, 23 -- Tue, 4 Apr 2006 08:52:08 -0400
4, 23 -- Received: from [141.209.160.132] ([141.209.160.132]) by egateb.central.cmich.local with Microsoft SMTPSVC(5.0.2195.6713);
4, 23 -- Tue, 4 Apr 2006 08:14:07 -0400
4, 23 -- Mime-Version: 1.0
4, 23 -- Message-Id: {f06230905c058111a17a7-at-[141.209.160.132]}
4, 23 -- In-Reply-To: {200604040525.k345PRid000860-at-ns.microscopy.com}
4, 23 -- References: {200604040525.k345PRid000860-at-ns.microscopy.com}
4, 23 -- Date: Tue, 4 Apr 2006 08:14:28 -0400
4, 23 -- To: Colin.Veitch-at-csiro.au
4, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
4, 23 -- Subject: Re: [Microscopy] Problem with a Philips EM410 TEM
4, 23 -- Cc: Microscopy-at-microscopy.com
4, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
4, 23 -- X-OriginalArrivalTime: 04 Apr 2006 12:14:07.0069 (UTC) FILETIME=[456D58D0:01C657E1]
4, 23 -- X-CanItPRO-Stream: default
4, 23 -- X-Spam-Score: -4 () L_EXCH_MF
4, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21
==============================End of - Headers==============================




From: tonygr-at-MIT.EDU
Date: Tue, 4 Apr 2006 08:09:08 -0500
Subject: [Microscopy] Re: help for MgO prepration and Fe oxidation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I don't quite understand the question you are asking about specimen
preparation - you say "cross-section", but cross section of what? -
so I won't address that part of your post.

I also don't understand where the Fe comes from. However, I do know
that over a period of (many) months, MgO samples transform into what
appears to be MgCO3. For a period of years, I had a student working
on polycystalline MgO. He made samples by ion milling. He did not
have to take special precautions with his samples if he examined them
days or weeks after they were made, but if we wanted to look at them
again a year or two later we had to "tickle" them with the ion mill
to clean them up. STEM analysis showed that the carbonate layer had
formed. We did not try to prevent this happening as it was not a
major problem.

MgO is known to be beam sensitive. A significant part of the damage
depends on the current density, so working in a FEG-STEM you can be
much worse-off than imaging or using a less intense electron
probe. But you can't eliminate the problem. Going to lower bean
voltages can (counter-intuitively) make the problem worse, as the
cross-section for various electron-sample interactions can actually
increase at lower voltages - though the total current in the electron
probe will also decrease, which may partially or totally compensate.

Tony Garratt-Reed

} At 10:24 PM 4/3/2006, you wrote:


Email: chao.wang-at-materials.ox.ac.uk
Name: Chao Wang

Organization: Oxford Materials

Title-Subject: [Filtered] help for MgO prepration and Fe oxidation

Question: Dear All

I have three problems, could you tell me some suggestions:

1.single crystale MgO preparation

I need very good quality cross section TEM sample. I now manully
polish it to 70 micron and dimple to abount 20 micron and then ion
milling (PIPS). But it's not so good. The edge looks sharp and MgO
cracks a lot when I ground lower then 60 micron on diamond papers. Do
you have better ideas to get very thin specimen?

2. Fe oxidiation
Another problem is Fe oxidation (on top of MgO substrate), it seems
after some time, the sample oxidized a lot, even I got very thin
specimen, it looks like amorphous (it should be crystalline). How to
keep it? (I use plasma cleaning, not working very well)

3. Beam sensitive to MgO sample

Sometime the beam in TEM is sensitive to MgO sample, how to get rid of this?

Your help is very appreicated!

best wishes

Chao

***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA

Tel: 617-253-4622
Fax: 617-258-6478
************************************************


==============================Original Headers==============================
22, 27 -- From tonygr-at-MIT.EDU Tue Apr 4 08:09:07 2006
22, 27 -- Received: from biscayne-one-station.mit.edu (BISCAYNE-ONE-STATION.MIT.EDU [18.7.7.80])
22, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k34D97Cn006158
22, 27 -- for {MICROSCOPY-at-MICROSCOPY.COM} ; Tue, 4 Apr 2006 08:09:07 -0500
22, 27 -- Received: from outgoing.mit.edu (OUTGOING-AUTH.MIT.EDU [18.7.22.103])
22, 27 -- by biscayne-one-station.mit.edu (8.13.6/8.9.2) with ESMTP id k34D6Rbk020108;
22, 27 -- Tue, 4 Apr 2006 09:06:28 -0400 (EDT)
22, 27 -- Received: from emlab.mit.edu (EMLAB.MIT.EDU [18.82.0.121])
22, 27 -- (authenticated bits=0)
22, 27 -- (User authenticated as tonygr-at-ATHENA.MIT.EDU)
22, 27 -- by outgoing.mit.edu (8.13.6/8.12.4) with ESMTP id k34D6Nue020175
22, 27 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT);
22, 27 -- Tue, 4 Apr 2006 09:06:26 -0400 (EDT)
22, 27 -- Message-Id: {6.2.3.4.2.20060404085115.01d7e4e8-at-po9.mit.edu}
22, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4
22, 27 -- Date: Tue, 04 Apr 2006 09:06:32 -0400
22, 27 -- To: chao.wang-at-materials.ox.ac.uk, MICROSCOPY-at-MICROSCOPY.COM
22, 27 -- From: Anthony Garratt-Reed {tonygr-at-MIT.EDU}
22, 27 -- Subject: Re: [Microscopy] help for MgO prepration and Fe oxidation
22, 27 -- In-Reply-To: {200604040224.k342OIvj011960-at-ns.microscopy.com}
22, 27 -- References: {200604040224.k342OIvj011960-at-ns.microscopy.com}
22, 27 -- Mime-Version: 1.0
22, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
22, 27 -- X-Spam-Score: 1.217
22, 27 -- X-Spam-Level: * (1.217)
22, 27 -- X-Spam-Flag: NO
22, 27 -- X-Scanned-By: MIMEDefang 2.42
==============================End of - Headers==============================




From: andromeda_tm-at-libero.it
Date: Tue, 4 Apr 2006 08:34:15 -0500
Subject: [Microscopy] viaWWW: Cutting angle with a flat razor blade

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both andromeda_tm-at-libero.it as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: andromeda_tm-at-libero.it
Name: Massimo TOSI

Organization: None

Title-Subject: [Filtered] Cutting angle with a flat razor blade

Question: Hi all,

I wonder if someone could give me some advice about the best cutting angle for specimens (mouse tissues) embedded into wax (Paraplast - melting point 56ƒC) at room temperature (about 18ƒC) with a microtome flat razor blade.
Thank you in advance.
Best Regards,

Massimo


---------------------------------------------------------------------------


==============================Original Headers==============================
10, 14 -- From zaluzec-at-microscopy.com Tue Apr 4 08:34:15 2006
10, 14 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
10, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k34DYE6o016109
10, 14 -- for {microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 08:34:14 -0500
10, 14 -- Mime-Version: 1.0
10, 14 -- X-Sender: (Unverified)
10, 14 -- Message-Id: {p06110400c05826409a1f-at-[206.69.208.22]}
10, 14 -- Date: Tue, 4 Apr 2006 08:34:13 -0500
10, 14 -- To: microscopy-at-microscopy.com
10, 14 -- From: andromeda_tm-at-libero.it (by way of MicroscopyListserver)
10, 14 -- Subject: viaWWW: Cutting angle with a flat razor blade
10, 14 -- Content-Type: text/plain; charset="iso-8859-1"
10, 14 -- Content-Transfer-Encoding: 8bit
10, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k34DYE6o016109
==============================End of - Headers==============================




From: sergei2-at-ornl.gov
Date: Tue, 4 Apr 2006 08:48:53 -0500
Subject: [Microscopy] FW: Resolution and information theory

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Good morning, everybody
            I am looking for advice on defining resolution and minimal feature size through information theory. In 1992, there was a paper by D. Van Dyck and A.F. de Jong (Ultramicroscopy 47, 266 (1992)), which addressed in some detail general principles on information theory as applied to image formation mechanism in electron microscopy. I am wondering what the current status of this field is.
            Thank you in advance
            Sergei  
 
 
--
Sergei V. Kalinin,
Oak Ridge National Laboratory,
1 Bethel Valley Rd,
Bldg. 3025, MS6030,
Oak Ridge, TN 37831
Phone: (865) 241-0236
FAX: (865) 574-4143
URL: sergei2.kalininweb.com
New: http://nanotransport.ornl.gov
 


==============================Original Headers==============================
2, 30 -- From sergei2-at-ornl.gov Tue Apr 4 08:48:53 2006
2, 30 -- Received: from emroute2.ornl.gov (emroute2.ornl.gov [160.91.86.17])
2, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k34Dmrqd023746
2, 30 -- for {microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 08:48:53 -0500
2, 30 -- Received: from emroute2.ornl.gov (localhost [127.0.0.1])
2, 30 -- by emroute2.ornl.gov (PMDF V6.2-1x9 #31038)
2, 30 -- with ESMTP id {0IX7000HIADGZ1-at-emroute2.ornl.gov} for
2, 30 -- microscopy-at-microscopy.com; Tue, 04 Apr 2006 09:48:53 -0400 (EDT)
2, 30 -- Received: from ORNLEXCHANGE.ornl.gov (ornlexchange2.ornl.gov [160.91.1.22])
2, 30 -- by emroute2.ornl.gov (PMDF V6.2-1x9 #31038)
2, 30 -- with ESMTP id {0IX700620ADG6P-at-emroute2.ornl.gov} for
2, 30 -- microscopy-at-microscopy.com; Tue, 04 Apr 2006 09:48:52 -0400 (EDT)
2, 30 -- Date: Tue, 04 Apr 2006 09:48:50 -0400
2, 30 -- From: "Kalinin, Sergei V." {sergei2-at-ornl.gov}
2, 30 -- Subject: FW: Resolution and information theory
2, 30 -- To: microscopy-at-microscopy.com
2, 30 -- Message-id: {3E4D6911DB2CA94DA31AC5E1CB522DBB42AEAF-at-ORNLEXCHANGE.ornl.gov}
2, 30 -- MIME-version: 1.0
2, 30 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5
2, 30 -- Content-type: text/plain; charset=iso-8859-1
2, 30 -- Importance: high
2, 30 -- Priority: Urgent
2, 30 -- X-Priority: 1
2, 30 -- Thread-Topic: Resolution and information theory
2, 30 -- Thread-Index: AcZX7Nd9csa8AnjnTyq53Z4QkequswAAZdTA
2, 30 -- Content-class: urn:content-classes:message
2, 30 -- X-MS-Has-Attach:
2, 30 -- X-MS-TNEF-Correlator:
2, 30 -- Content-Transfer-Encoding: 8bit
2, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k34Dmrqd023746
==============================End of - Headers==============================




From: raristau-at-ims.uconn.edu
Date: Tue, 4 Apr 2006 08:55:32 -0500
Subject: [Microscopy] Re: Problem with a Philips EM410 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Colin,

Based on my experience with a Philips EM 420, I have found the instrument to
be very sensitive to having proper water and pneumatic pressure.

I would first check the pneumatics to ensure that it is giving proper
pressure. Ours needs about 80 psi, or we get exactly the problem that you
describe. Unfortunately, we have that problem a lot because we rely on
'house' air and have no control over the source.

If that doesn't solve the problem, thoroughly examine the water circuit. It
may look like "plenty" of water, but it may not be enough. First check and
clean the water filter near where the water line enters the back of the
scope. It never ceases to surprise me how big a difference that makes!

If that doesn't work, there may be water 'loops' inside that do not have
sufficient pressure. The only way to check is to take apart the water lines
one at a time and measure the flow of each. (I did that by timing the flow
into an open container. The manual will list the specs.) I have found that
the isolation values clog over time and need to be replaced. Or do what I
did and simply by-pass the faulty valves. If you do that, you will have to
manually stop the flow in the correct lines when you do a bake-out.

Good luck!

--
Roger A. Ristau, PhD
TEM Specialist
Institute of Materials Science
97 North Eagleville Road
University of Connecticut
Storrs, CT 06269
vox: 860-486-5379
fax: 860-486-4745




} Hi,
}
} I have a Philips 410 TEM which has decided not to cooperate! I am not
} overly familiar with this machine so I apologise if I have missed
} something obvious.
}
} When you turn on the system and begin the pumpdown sequence the rotary
} pump starts and after around 30 seconds (the vacuum gauge drops to
} around 10 on the scale) the rotary pump shuts off and that is it.
}
} The vacuum system indicators (valve status) on the side panel all remain
} off. I have checked all the fuses and they are OK. There is plenty of
} cooling water (going in and coming out). The diffusion pump heater
} seems to be OK (it is not open circuit, nor ohms resistance). The
} pneumatic gas pressure is also OK.
}
} Any help as to a possible cause/cure would be appreciated.
}
} Cheers.
}
} Colin Veitch
}
} Electron Microscopist
}
} CSIRO Textile and Fibre Technology
}
} PO Box 21, BELMONT, Vic. 3216. Australia.
}
} E-mail: colin.veitch-at-csiro.au
}
} Web: http://www.tft.csiro.au
}
} Tel: +61 (0) 3 5246 4000
} Mob: 0438 538 475
} Fax: +61 (0) 3 5246 4811
}
}
}
} The information contained in this e-mail message may be privileged or
} confidential information. If you are not an intended recipient, you may
} not copy, distribute or take any action in reliance on it. If you have
} received this message in error, please telephone CSIRO Textile and Fibre
} Technology on +61 3 5246 4000.
}
}
}
} ==============================Original Headers==============================
} 17, 30 -- From Colin.Veitch-at-csiro.au Tue Apr 4 00:20:22 2006
} 17, 30 -- Received: from vic-MTAout2.csiro.au (vic-MTAout2.csiro.au
} [150.229.64.38])
} 17, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} k345KLVZ025429
} 17, 30 -- for {microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 00:20:21 -0500
} 17, 30 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns;
} b=dBF7dfKXSlt81lkUABrPqUbsCIwjoURW1HJ9hzb0jZaxVI1s/fKCKoicAEX1Lot9kXcu1OaMU4mh
} pGKGJD9rj0Yt5OO80G/XIVKO1w6MjYmSjaEgTZ03epxNTfKPHGmp;
} 17, 30 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56])
} 17, 30 -- by vic-MTAout2.csiro.au with ESMTP; 04 Apr 2006 15:20:20 +1000
} 17, 30 -- X-IronPort-AV: i="4.03,160,1141563600";
} 17, 30 -- d="scan'208"; a="73879638:sNHT25511800"
} 17, 30 -- Received: from exvicn1-mel.nexus.csiro.au ([138.194.3.60]) by
} exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713);
} 17, 30 -- Tue, 4 Apr 2006 15:20:19 +1000
} 17, 30 -- Received: from exvic5-gex.nexus.csiro.au ([138.194.194.6]) by
} exvicn1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713);
} 17, 30 -- Tue, 4 Apr 2006 15:20:19 +1000
} 17, 30 -- X-MIMEOLE: Produced By Microsoft Exchange V6.0.6603.0
} 17, 30 -- Content-Class: urn:content-classes:message
} 17, 30 -- MIME-Version: 1.0
} 17, 30 -- Content-Type: text/plain;
} 17, 30 -- charset="us-ascii"
} 17, 30 -- Subject: Problem with a Philips EM410 TEM
} 17, 30 -- Date: Tue, 4 Apr 2006 15:20:18 +1000
} 17, 30 -- Message-ID:
} {32CDDDAA7161394599F0025494915749024813-at-exvic5-gex.nexus.csiro.au}
} 17, 30 -- X-MS-Has-Attach:
} 17, 30 -- X-MS-TNEF-Correlator:
} 17, 30 -- Thread-Topic: Problem with a Philips EM410 TEM
} 17, 30 -- Thread-Index: AcZXp3YO7P7oXMhtRkmVma7B0e0d9A==
} 17, 30 -- From: {Colin.Veitch-at-csiro.au}
} 17, 30 -- To: {microscopy-at-microscopy.com}
} 17, 30 -- X-OriginalArrivalTime: 04 Apr 2006 05:20:19.0708 (UTC)
} FILETIME=[772AB3C0:01C657A7]
} 17, 30 -- Content-Transfer-Encoding: 8bit
} 17, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
} ns.microscopy.com id k345KLVZ025429
} ==============================End of - Headers==============================


==============================Original Headers==============================
12, 20 -- From raristau-at-ims.uconn.edu Tue Apr 4 08:55:32 2006
12, 20 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204])
12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k34DtWZC031023
12, 20 -- for {microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 08:55:32 -0500
12, 20 -- Received: from [137.99.44.238] (d44h238.public.uconn.edu [137.99.44.238])
12, 20 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id k34DtHDJ031700
12, 20 -- for {microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 09:55:18 -0400
12, 20 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0
12, 20 -- Date: Tue, 04 Apr 2006 09:54:52 -0400
12, 20 -- Subject: Re: [Microscopy] Problem with a Philips EM410 TEM
12, 20 -- From: Roger Ristau {raristau-at-ims.uconn.edu}
12, 20 -- To: {microscopy-at-microscopy.com}
12, 20 -- Message-ID: {C057F2EC.B25%raristau-at-ims.uconn.edu}
12, 20 -- In-Reply-To: {200604040525.k345PveJ002113-at-ns.microscopy.com}
12, 20 -- Mime-version: 1.0
12, 20 -- Content-type: text/plain; charset="US-ASCII"
12, 20 -- Content-transfer-encoding: 7bit
12, 20 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information.
12, 20 -- X-UConn-MailScanner: Found to be clean
12, 20 -- X-UConn-MailScanner-SpamCheck:
==============================End of - Headers==============================




From: keith.morris-at-ucl.ac.uk
Date: Tue, 4 Apr 2006 09:56:45 -0500
Subject: [Microscopy] Microscopy] LM - OX3

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Caroline has usefully pointed out that 'miXscope' software is now available
for the fun 'Digital Blue' QX5 children's microscope, which is an upgrade of
the older 'Intel' QX3 plastic microscope. Both are used in many homes and
schools. I suspect Caroline is an Apple user, as the highly regarded
miXscope software allows you to use the QX-5 (and the QX-3 and the MiScope
USB) with an Apple G3 to G5 computer - for $15 single, $20 schoolwide.

The QX-5 [and QX-3] is supplied with Windows only software and otherwise
won't work at all with an Apple MAC. At present miXscope doesn't seem to
support the new Intel version Apples though, and it requires Mac OS X 10.2.8
or later.

For those interested, I attach my 'Amazon.co.uk' review of the kid friendly
QX-5 microscope, based on my experience with my kid's one. The QX-5 site is
at http://www.playdigitalblue.com. Plus there's the superb
micro.magnet.fsu.edu/optics/intelplay site for the QX-3 (they are promising
to update it for the QX-5). There is also some QX-5 help forum at
http://www.expansys.com.

Regards

Keith


My amazon.co.uk Review of the Digital Blue QX-5 'microscope'.

This is a rather fun toy microscope that has a built in CMOS detector so
that images can only be viewed via a Windows PC. The all plastic
construction (including lenses) limits the accuracy of focussing and the
on-screen image resolution is adequate rather than good. This microscope was
originally marketed by Intel and built by toy manufacturer Mattel as the
QX-3. Now Digital Blue have taken it on after Intel discontinued production.
The QX-5 is an upgrade having 640 x 480 pixel resolution rather than just
352 x 288 in the original QX-3. Have a look at
micro.magnet.fsu.edu/optics/intelplay for very detailed scientific
description of the original QX-3 and advice on what to use it for. Every
school in the UK was given one of these in 2002.

I installed the QX-5 software under Windows XP Pro on a 1.2MHz Athlon PC and
the software worked fine. The only downside is that the software changes the
CRT screen refresh rate to 60Hz and doesn't switch it back to the flicker
free 85Hz. So a trip to 'Start, Control Panel, Display, Settings, Advanced,
Monitor' is required to set the graphics back to their correct setting
(check these before you run the software). Otherwise the software and USB
microscope run very well. It comes with a small prepared 'slide' (a
cardboard and plastic array of things like insect parts) plus a reasonable
archive of digital images which you can add to.

Once on the PC the 640x480 images can be manipulated and pasted etc, and it
does time-lapse for things like crystal growth (but there's not much control
of the time-lapse intervals). I have a QX-5 at home for the kids, but like
most kids with microscopes they can get bored with it after running out of
things to view - so web and book searches for ideas is useful.

The QX-5 has not got the resolution of even a standard 'school' compound
microscope though, largely because you see it all 'enlarged' on a large
computer screen, it uses plastic lenses and has a low resolution detector
(but you can share the view with friends). So you may find the QX-5 a real
disappointment if you expect too much of it in terms of image quality.
However it is rather fun to use and has transmission + reflection white LED
light sources built in to view specimens. The software is also very kid
friendly and the increased resolution over the QX-3 is very welcome. So
overall, recommended for pre-teen budding scientists.




----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk



-----Original Message-----
X-from: schooley-at-mcn.org [mailto:schooley-at-mcn.org]
Sent: 02 April 2006 19:04
To: keith.morris-at-ucl.ac.uk

Some of you listers enjoy using the inexpensive children's digital
microscope, the QX3, as a home hobby item. It's gone thru several
changes, but it's still available, with improved software. There's a
new software package, available at
http://www.edhsw.com/mixscope/index.html

I have no interest in the company, and no personal opinion on the
product; I just want you to have fun...
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO

==============================Original Headers==============================
2, 16 -- From schooley-at-mcn.org Sun Apr 2 12:59:08 2006
2, 16 -- Received: from dns3.mcn.org (dns3.mcn.org [216.150.240.32])
2, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
k32Hx8s4002705
2, 16 -- for {microscopy-at-microscopy.com} ; Sun, 2 Apr 2006 12:59:08
-0500
2, 16 -- Received: from [66.42.18.122] (helo=[10.0.1.2])
2, 16 -- by dns3.mcn.org with esmtpa (Exim 4.60)
2, 16 -- (envelope-from {schooley-at-mcn.org} )
2, 16 -- id IX3WMJ-000LAN-39; Sun, 02 Apr 2006 10:59:07 -0700
2, 16 -- Mime-Version: 1.0
2, 16 -- Message-Id: {a06200701c055be59a1a0-at-[10.0.1.2]}
2, 16 -- Date: Sun, 2 Apr 2006 10:56:56 -0700
2, 16 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com}
2, 16 -- From: Caroline Schooley {schooley-at-mcn.org}
2, 16 -- Subject: Microscopy] LM - OX3
2, 16 -- Cc: diamondsci-at-charter.net, schooley-at-mac.com
2, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================



==============================Original Headers==============================
26, 26 -- From keith.morris-at-ucl.ac.uk Tue Apr 4 09:56:45 2006
26, 26 -- Received: from vscani-a.ucl.ac.uk (vscani-a.ucl.ac.uk [144.82.108.29])
26, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k34EuiJ5013742
26, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 09:56:44 -0500
26, 26 -- Received: from cell23.ioo.ucl.ac.uk ([144.82.134.123] helo=keithhigrade)
26, 26 -- by vscani-a.ucl.ac.uk with esmtp (Exim 4.60)
26, 26 -- (envelope-from {keith.morris-at-ucl.ac.uk} )
26, 26 -- id 1FQmxV-0005J2-Uy
26, 26 -- for Microscopy-at-microscopy.com; Tue, 04 Apr 2006 15:56:42 +0100
26, 26 -- From: "Keith J Morris" {keith.morris-at-ucl.ac.uk}
26, 26 -- To: {Microscopy-at-microscopy.com}
26, 26 -- Subject: RE: [Microscopy] Digital Blue QX5
26, 26 -- Date: Tue, 4 Apr 2006 15:55:53 +0100
26, 26 -- Message-ID: {000001c657f7$ded138f0$7b865290-at-keithhigrade}
26, 26 -- MIME-Version: 1.0
26, 26 -- Content-Type: text/plain;
26, 26 -- charset="us-ascii"
26, 26 -- Content-Transfer-Encoding: 7bit
26, 26 -- X-Mailer: Microsoft Office Outlook 11
26, 26 -- Thread-Index: AcZWf83JPQah9yzESnqkYJFWEzw2+gBc+PHg
26, 26 -- In-Reply-To: {200604021803.k32I3poj017375-at-ns.microscopy.com}
26, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2670
26, 26 -- X-UCL-MailScanner-Information: Please contact the UCL Helpdesk, helpdesk-at-ucl.ac.uk for more information
26, 26 -- X-UCL-MailScanner: Found to be clean
26, 26 -- X-UCL-MailScanner-SpamCheck:
26, 26 -- X-MailScanner-From: keith.morris-at-ucl.ac.uk
==============================End of - Headers==============================




From: murraytm-at-u.washington.edu
Date: Tue, 4 Apr 2006 10:38:18 -0500
Subject: [Microscopy] Staining of Polymers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I have a student who is looking at particles with a polystyrene core
and a polyamine shell. He is interested in staining the particles so
that he can distinguish the two layers. Presently the particles are
on the order of 0.5 micron diameter but he is hoping to reduce this
down to a few tens of nm.

It sounds like he has some references that used either OsO4 or RuO4
as stains.

I understand that these stains are commonly used in the biology
world, I am a materials person and have no experience with these.
I'm looking for a couple pieces of info:

1) Recommendations for preferentially staining either the core or the
shell. (With the above stains or something else)

2) Cautions for using these stains, I understand they are quite toxic.

3) A reference which discusses all this would be great.

The student talks about looking at these in the SEM, but I think the
TEM will be more effective, specially if he manages to get his
particles as small as he wants. Is there a way to image the core vs
the shell in the SEM? I have a FESEM so it might be possible to
image the small particles he anticipates, but I don't think I can get
an image of the core through a 0.125 micron shell. Thoughts?

Thanks for your help.

Tom

------------------------------------------------------------------------
---------------------------------------------
Thomas M Murray email:
murraytm-at-u.washington.edu
Electron Microscopy Center Manager Phone: (206)543-2836
Materials Science & Engineering Fax: (206)543-3100
Box 352120 302 Roberts Hall Cell: (425)345-0083
University of Washington
Seattle, WA 98195


==============================Original Headers==============================
11, 24 -- From murraytm-at-u.washington.edu Tue Apr 4 10:38:18 2006
11, 24 -- Received: from mxout5.cac.washington.edu (mxout5.cac.washington.edu [140.142.32.135])
11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k34FcHCQ023942
11, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 10:38:18 -0500
11, 24 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.139])
11, 24 -- by mxout5.cac.washington.edu (8.13.6+UW06.03/8.13.5+UW06.03) with ESMTP id k34FbxIf022845
11, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK)
11, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 08:38:08 -0700
11, 24 -- X-Auth-Received: from [128.95.118.89] (tstoebe-mse.ad.engr.washington.edu [128.95.118.89])
11, 24 -- (authenticated authid=murraytm)
11, 24 -- by smtp.washington.edu (8.13.6+UW06.03/8.13.5+UW06.03) with ESMTP id k34FbxI1017103
11, 24 -- (version=TLSv1/SSLv3 cipher=RC4-SHA bits=128 verify=NOT)
11, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 08:37:59 -0700
11, 24 -- Mime-Version: 1.0 (Apple Message framework v749.3)
11, 24 -- X-Priority: 3
11, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
11, 24 -- Message-Id: {2575642D-F6A1-41C1-A8D4-B930EFFA8276-at-u.washington.edu}
11, 24 -- Content-Transfer-Encoding: 7bit
11, 24 -- From: Tom Murray {murraytm-at-u.washington.edu}
11, 24 -- Subject: Staining of Polymers
11, 24 -- Date: Tue, 4 Apr 2006 08:37:55 -0700
11, 24 -- To: "Microscopy ListServer (E-mail)" {Microscopy-at-microscopy.com}
11, 24 -- X-Mailer: Apple Mail (2.749.3)
11, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CP_MEDIA_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __HAS_X_PRIORITY 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_CRUFT 0'
==============================End of - Headers==============================




From: walck-at-southbaytech.com
Date: Tue, 4 Apr 2006 11:54:46 -0500
Subject: [Microscopy] viaWWW: help for MgO prepration and Fe oxidation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I think that I can address all of your questions to some extent:

1)
For the preparation of MgO by ion milling, you might consider the
sequence of sample preparation espoused by Arpa Barna and
Technoorg-Linde where samples are ground very thin in special titanium
grids made for cross section and using low angle milling. The complete
process is given in The Handbook of Microscopy, Applications in
Materials Science, Solid-State Physics, and Chemistry, edited by S.
Amelinckx, D. van Dyck, J. van Landuyt, G.van Tendeloo, and published by
VCH. Technoorg-Linde has a suite of instruments that are very useful
for cutting and mounting the samples for this process. This technique
will minimize surface thickness variations of different materials in a
cross section due to the geometrical factors affecting ion milled
samples.

I know that I have tried the small angle cleavage technique with MgO in
the past, but I can't remember whether I had success with it or not. If
anyone has an example of a TEM sample prepared by cleaving single
crystal MgO, I would be particularly interested in see those results.
If you are interested, we have the MicroCleave(TM) sample preparation
kit available.

2)
It is not surprising that a vacuum does not stop the oxidation process
of Fe. Water on the surface is important in the oxidation of iron
through the formation of hydroxyl species. Unless your vacuum is an
ultra high vacuum system and has been baked, you will always have water
absorbed on the surface. A desiccator that has only been rough pumped
or (heaven forbid) pumped using a house vacuum has plenty of both oxygen
and water available for oxidation. We have introduced a new product
called the SampleSaver(TM) Storage Container that will help in the
prevention of oxidation of samples. This unit uses an inert gas such as
Ar, N2, or CO2 to purge the volume of the container and then to compress
the inert gas slightly so to prevent diffusion into the container. I
have used this successfully with XTEM samples that readily oxidized when
made, but I have not tried it with Fe samples. We have a holder
specially designed for TEM samples. Contact me offline to discuss your
problem.

3)
There are a few things that can help with beam sensitive samples. When
I was with PPG, I had some problems with glass samples. First, what
accelerating voltage are you using? Increasing the voltage will
decrease the deposition of energy into the sample and will help stop
beam damage. 200 keV should be considered the minimum that you should
use. For the same reason, thinner samples will help. When I was forced
to use a 120 keV machine for the glass samples, I had some success with
putting a thin carbon coating on the two surfaces. I used both
evaporation and ion sputtering to put the carbon down. It is important
to put the right amount down, too much interferes too much with your
imaging and too little doesn't do much to help. I modified the
configuration of my ion mill for ion depositing the carbon by
sputtering. Alternatively, you could use a commercially available ion
sputter deposition system for high resolution SEM such as our IBS/e
system for putting a controlled uniform layer of carbon on the sample.

Disclaimer:
South Bay Technology (SBT), Inc. is the representative for
Technoorg-Linde products in the United States. SBT manufactures and
sells the MicroCleave(TM) kit, SampleSaver(TM) Storage Container, and
the IBS/e ion beam sputter deposition and etching system.



-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: chao.wang-at-materials.ox.ac.uk [mailto:chao.wang-at-materials.ox.ac.uk]

Sent: Monday, April 03, 2006 7:20 PM
To: Walck-at-SouthBayTech.com

This Question/Comment was submitted to the Microscopy Listserver using
the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both chao.wang-at-materials.ox.ac.uk as well as the
MIcroscopy Listserver
------------------------------------------------------------------------
---

Email: chao.wang-at-materials.ox.ac.uk
Name: Chao Wang

Organization: Oxford Materials

Title-Subject: [Filtered] help for MgO prepration and Fe oxidation

Question: Dear All

I have three problems, could you tell me some suggestions:

1.single crystale MgO preparation

I need very good quality cross section TEM sample. I now manully polish
it to 70 micron and dimple to abount 20 micron and then ion milling
(PIPS). But it's not so good. The edge looks sharp and MgO cracks a lot
when I ground lower then 60 micron on diamond papers. Do you have better
ideas to get very thin specimen?

2. Fe oxidiation
Another problem is Fe oxidation (on top of MgO substrate), it seems
after some time, the sample oxidized a lot, even I got very thin
specimen, it looks like amorphous (it should be crystalline). How to
keep it? (I use plasma cleaning, not working very well)

3. Beam sensitive to MgO sample

Sometime the beam in TEM is sensitive to MgO sample, how to get rid of
this?

Your help is very appreicated!

best wishes

Chao

------------------------------------------------------------------------
---

==============================Original
Headers==============================
15, 12 -- From zaluzec-at-microscopy.com Mon Apr 3 21:11:17 2006 15, 12 --
Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
15, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id k342BGYK026336
15, 12 -- for {microscopy-at-microscopy.com} ; Mon, 3 Apr 2006
21:11:17 -0500
15, 12 -- Mime-Version: 1.0
15, 12 -- X-Sender: (Unverified)
15, 12 -- Message-Id: {p06110402c057863616fc-at-[206.69.208.22]}
15, 12 -- Date: Mon, 3 Apr 2006 21:11:15 -0500
15, 12 -- To: microscopy-at-microscopy.com
15, 12 -- From: chao.wang-at-materials.ox.ac.uk (by way of
MicroscopyListserver) 15, 12 -- Subject: viaWWW: help for MgO prepration
and Fe oxidation 15, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of -
Headers==============================


==============================Original Headers==============================
32, 27 -- From walck-at-southbaytech.com Tue Apr 4 11:54:44 2006
32, 27 -- Received: from ylpvm12.prodigy.net (ylpvm12-ext.prodigy.net [207.115.57.43])
32, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k34GsgUd002016
32, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 11:54:43 -0500
32, 27 -- Received: from pimout6-ext.prodigy.net (pimout6-int.prodigy.net [207.115.4.22])
32, 27 -- by ylpvm12.prodigy.net (8.12.10 outbound/8.12.10) with ESMTP id k34GsYbL028578
32, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 12:54:35 -0400
32, 27 -- X-ORBL: [64.169.193.90]
32, 27 -- Received: from dynamicbl8uno3 (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90])
32, 27 -- by pimout6-ext.prodigy.net (8.13.4 outbound domainkey aix/8.13.4) with ESMTP id k34GsDbh025970;
32, 27 -- Tue, 4 Apr 2006 12:54:34 -0400
32, 27 -- From: "Scott Walck" {walck-at-southbaytech.com}
32, 27 -- To: {chao.wang-at-materials.ox.ac.uk}
32, 27 -- Cc: {Microscopy-at-microscopy.com}
32, 27 -- Subject: RE: [Microscopy] viaWWW: help for MgO prepration and Fe oxidation
32, 27 -- Date: Tue, 4 Apr 2006 09:54:17 -0700
32, 27 -- Message-ID: {002001c65808$788dcf20$7801a8c0-at-dynamicbl8uno3}
32, 27 -- MIME-Version: 1.0
32, 27 -- Content-Type: text/plain;
32, 27 -- charset="us-ascii"
32, 27 -- Content-Transfer-Encoding: 7bit
32, 27 -- X-Priority: 3 (Normal)
32, 27 -- X-MSMail-Priority: Normal
32, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.2627
32, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
32, 27 -- In-Reply-To: {200604040220.k342KKuf006805-at-ns.microscopy.com}
32, 27 -- Importance: Normal
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 4 Apr 2006 18:17:49 -0500
Subject: [Microscopy] Small vacuum box

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Listers:

I am looking for a small (lunch box size) vacuum
container. This is for transporting SEM specimens
under vacuum to avoid oxidation between sites.

I will be using a small diaphram or rotary pump with
KF15 fitting. The unit does not have to hold initial
vacuum for a long time. Plastic or metal is not a key
factor but durability is in construction and long term use.

Any ideas?

Vendor input is welcome.

gary g.


==============================Original Headers==============================
7, 17 -- From gary-at-gaugler.com Tue Apr 4 18:17:49 2006
7, 17 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
7, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k34NHlmV017348
7, 17 -- for {microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 18:17:48 -0500
7, 17 -- Received: (qmail 8197 invoked from network); 4 Apr 2006 16:16:48 -0700
7, 17 -- Received: by simscan 1.1.0 ppid: 8194, pid: 8195, t: 0.1041s
7, 17 -- scanners: regex: 1.1.0 attach: 1.1.0
7, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211)
7, 17 -- by qsmtp2 with SMTP; 4 Apr 2006 16:16:47 -0700
7, 17 -- Message-Id: {7.0.1.0.2.20060404161341.023138a0-at-gaugler.com}
7, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0
7, 17 -- Date: Tue, 04 Apr 2006 16:17:43 -0700
7, 17 -- To: MSA listserver {microscopy-at-microscopy.com}
7, 17 -- From: Gary Gaugler {gary-at-gaugler.com}
7, 17 -- Subject: Small vacuum box
7, 17 -- Mime-Version: 1.0
7, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: walck-at-southbaytech.com
Date: Tue, 4 Apr 2006 19:56:24 -0500
Subject: [Microscopy] Small vacuum box

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Gary,
I have tested our new SampleSaver(TM) Storage Container under vacuum
with a diaphragm pump and it holds a vacuum quite well for a number of
days (length of test). However, I have not tested it whether it will
protect samples from oxidation as well as purging the unit with N2 and I
have also not checked the pressure change during that time. We have a
number of inserts for the unit that will hold 1/8" pin SEM stubs and
3/8" SEM stubs as well as one that will hold three TEM grid boxes.
There is a insert unit available that will hold a 1-1/4" metallurgical
sized sample. The different SEM sample plates can be mixed and matched.
We have a larger unit that was designed for holding FIB Lift-out samples
for storage and transport that will also accept the 1/8" pins. The
units are light and perfect for transporting samples from site to site.

The advantage that I found for purging is that there is not always a
vacuum pump available at the other end of your trip when transporting
samples. In fact, there isn't always a spare pure Ar or N2 gas line
available either. That's why we also designed the Thing-A-Ma-Jug(TM)
gas supply system that will purge the unit with the head space from
liquid nitrogen which almost always available in the typical EM lab and
is also a source of pure N2. The Thing-A-Ma-Jug(TM) is also light
enough to be very portable. The SampleSaver(TM) is designed to
pressurize the container when the purging is done and thus inhibit gas
diffusion through the plastic container and O-rings.

If you have any questions on the system, please give me a call.

Disclaimer:
SBT makes and sells the SampleSaver(TM) storage container system and
Thing-A-Ma-Jug(TM) gas supply system.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com]
Sent: Tuesday, April 04, 2006 4:25 PM
To: Walck-at-SouthBayTech.com

Listers:

I am looking for a small (lunch box size) vacuum
container. This is for transporting SEM specimens
under vacuum to avoid oxidation between sites.

I will be using a small diaphram or rotary pump with
KF15 fitting. The unit does not have to hold initial
vacuum for a long time. Plastic or metal is not a key
factor but durability is in construction and long term use.

Any ideas?

Vendor input is welcome.

gary g.


==============================Original
Headers==============================
7, 17 -- From gary-at-gaugler.com Tue Apr 4 18:17:49 2006
7, 17 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net
[66.60.130.145])
7, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
id k34NHlmV017348
7, 17 -- for {microscopy-at-microscopy.com} ; Tue, 4 Apr 2006
18:17:48 -0500
7, 17 -- Received: (qmail 8197 invoked from network); 4 Apr 2006
16:16:48 -0700 7, 17 -- Received: by simscan 1.1.0 ppid: 8194, pid:
8195, t: 0.1041s
7, 17 -- scanners: regex: 1.1.0 attach: 1.1.0
7, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211)
7, 17 -- by qsmtp2 with SMTP; 4 Apr 2006 16:16:47 -0700
7, 17 -- Message-Id: {7.0.1.0.2.20060404161341.023138a0-at-gaugler.com}
7, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0
7, 17 -- Date: Tue, 04 Apr 2006 16:17:43 -0700
7, 17 -- To: MSA listserver {microscopy-at-microscopy.com}
7, 17 -- From: Gary Gaugler {gary-at-gaugler.com}
7, 17 -- Subject: Small vacuum box
7, 17 -- Mime-Version: 1.0
7, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of -
Headers==============================


==============================Original Headers==============================
20, 27 -- From walck-at-southbaytech.com Tue Apr 4 19:56:24 2006
20, 27 -- Received: from ylpvm01.prodigy.net (ylpvm01-ext.prodigy.net [207.115.57.32])
20, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k350uOEU028572
20, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 19:56:24 -0500
20, 27 -- Received: from pimout6-ext.prodigy.net (pimout6-int.prodigy.net [207.115.4.22])
20, 27 -- by ylpvm01.prodigy.net (8.12.10 outbound/8.12.10) with ESMTP id k350uLai022661
20, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Apr 2006 20:56:21 -0400
20, 27 -- X-ORBL: [64.169.193.90]
20, 27 -- Received: from dynamicbl8uno3 (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90])
20, 27 -- by pimout6-ext.prodigy.net (8.13.4 outbound domainkey aix/8.13.4) with ESMTP id k350uH66091756;
20, 27 -- Tue, 4 Apr 2006 20:56:22 -0400
20, 27 -- From: "Scott Walck" {walck-at-southbaytech.com}
20, 27 -- To: {gary-at-gaugler.com}
20, 27 -- Cc: {Microscopy-at-microscopy.com}
20, 27 -- Subject: RE: [Microscopy] Small vacuum box
20, 27 -- Date: Tue, 4 Apr 2006 17:56:23 -0700
20, 27 -- Message-ID: {006201c6584b$c5fe59d0$7801a8c0-at-dynamicbl8uno3}
20, 27 -- MIME-Version: 1.0
20, 27 -- Content-Type: text/plain;
20, 27 -- charset="us-ascii"
20, 27 -- Content-Transfer-Encoding: 7bit
20, 27 -- X-Priority: 3 (Normal)
20, 27 -- X-MSMail-Priority: Normal
20, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.2627
20, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
20, 27 -- In-Reply-To: {200604042324.k34NOWEc023825-at-ns.microscopy.com}
20, 27 -- Importance: Normal
==============================End of - Headers==============================




From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Wed, 5 Apr 2006 04:47:46 -0500
Subject: [Microscopy] Re: Small vacuum box

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Have a look at a vacuum components catalog, like Lesker, MDC, EVAC or
so. I have used KF40 or ISO-K pipes as container. You may find PVC, Al
alloy, or SS fittings. Durability is garanteed ! Can be cleaned with
solvants, baked for degasing, and some workshops can modify standard
components for cheap (directe weld a vaccum valve, for exemple). I have
a old zeolite foreline trap, which I will use next as container.

Hope it helps

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



gary-at-gaugler.com a écrit :

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
9, 30 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Apr 5 04:47:45 2006
9, 30 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.154])
9, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k359liAU013151
9, 30 -- for {microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 04:47:45 -0500
9, 30 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2])
9, 30 -- by mailhost.u-strasbg.fr (8.13.4/jtpda-5.5pre1) with ESMTP id k359lfWJ025271
9, 30 -- ; Wed, 5 Apr 2006 11:47:41 +0200 (CEST)
9, 30 -- Received: from [130.79.54.3] (odhinn.u-strasbg.fr [130.79.54.3])
9, 30 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id ED02F1000126;
9, 30 -- Wed, 5 Apr 2006 11:47:31 +0200 (CEST)
9, 30 -- Message-ID: {4433923F.3000501-at-ipcms.u-strasbg.fr}
9, 30 -- Date: Wed, 05 Apr 2006 11:47:43 +0200
9, 30 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr}
9, 30 -- User-Agent: Mozilla Thunderbird 1.0.7 (X11/20051011)
9, 30 -- X-Accept-Language: fr, en
9, 30 -- MIME-Version: 1.0
9, 30 -- To: gary-at-gaugler.com, Microscopy-at-microscopy.com
9, 30 -- Subject: Re: [Microscopy] Small vacuum box
9, 30 -- References: {200604042325.k34NPd5P025606-at-ns.microscopy.com}
9, 30 -- In-Reply-To: {200604042325.k34NPd5P025606-at-ns.microscopy.com}
9, 30 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
9, 30 -- Content-Transfer-Encoding: 8bit
9, 30 -- X-IPCMS-MailScanner: Found to be clean
9, 30 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr
9, 30 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-2.0.2 (mailhost.u-strasbg.fr [130.79.200.154]); Wed, 05 Apr 2006 11:47:41 +0200 (CEST)
9, 30 -- X-Virus-Scanned: ClamAV 0.88/1376/Wed Apr 5 07:51:25 2006 on mr4.u-strasbg.fr
9, 30 -- X-Virus-Status: Clean
9, 30 -- X-Spam-Status: No, score=-1.4 required=5.0 tests=ALL_TRUSTED,AWL
9, 30 -- autolearn=disabled version=3.1.0
9, 30 -- X-Spam-Checker-Version: SpamAssassin 3.1.0 (2005-09-13) on mr4.u-strasbg.fr
==============================End of - Headers==============================




From: gerd.leitinger-at-meduni-graz.at
Date: Wed, 5 Apr 2006 05:51:48 -0500
Subject: [Microscopy] Re: Leica EM-Stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Ronda,

We have had a similar experience as Chris with our EM stain.
We do use it regularly, though.

Gerd


Datum: Tue, 4 Apr 2006 06:15:36 -0500
An: Gerd.Leitinger-at-meduni-graz.at
Von: christopher.hayden-at-novartis.com
Antwort an: christopher.hayden-at-novartis.com
Betreff: [Microscopy] Re: viaWWW: Leica EM-Stain

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Good Morning, Rhonda:
}
} We have a Leica EM-Stain. We purchased it about a year ago in a
} quest to make our staining absolutely consistent, and in regard to that,
} it works wonderfully. Given all of the options for wetting times, stain
} times, stain temperatures, etc, it's pretty versatile and dead-on
} accurate, not to mention it frees up a lot of a technician's time!
} However, we're had nothing but problems with the unit. We've gone
} though 3 pumps already, numerous service calls, etc. In Leica's defense,
} they may have had a bad batch of stain (high amounts of precipitate),
} which is clogging up the pump and the filters. Speaking of the stain,
} you're "required" to use their pre-made stains, else you void the warranty
} on the unit. There's a bit of upkeep with the stainer as well, in that you
} must perform a cleaning cycle at least once a week (which only takes a few
} minutes to get running but someone still has to remember to do it), and it
} uses 3% Nitric acid for the cleaning cycle, which possibly adds another
} waste stream for your lab.
} All in all, it would be a great unit if there weren't so many
} problems with it. We may just have ended up with a lemon, though. Hard to
} say.
}
} -Chris
}
}
}
}
}
}
} rra-at-stowers-institute.org
} 04/03/2006 10:20 PM
} Please respond to rra
}
}
} To: Christopher Hayden/PH/Novartis-at-PH
} cc:
} Subject: [Microscopy] viaWWW: Leica EM-Stain
}
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both rra-at-stowers-institute.org as well as the MIcroscopy
} Listserver
} ---------------------------------------------------------------------------
}
} Email: rra-at-stowers-institute.org
} Name: Rhonda Allen
}
} Organization: Stowers Institute
}
} Title-Subject: [Filtered] Leica EM-Stain
}
} Question: Hello, I am interested in purchasing the Leica EM-Stain. Does
} anyone have any comments, positive and negative, that I should take into
} consideration before making such an investment? We will be using it on
} formvar coated slot grids. Thanks in advance, Rhonda Allen BA HT(ASCP)HTL,
} QIHC Stowers Institute for Medical Research Kansas City, Missouri
} 816-926-4346
}
}
} ---------------------------------------------------------------------------
}
} ==============================Original
} Headers==============================
} 7, 12 -- From zaluzec-at-microscopy.com Mon Apr 3 21:12:03 2006
} 7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} [206.69.208.22])
} 7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id k342C2tI026972
} 7, 12 -- for {microscopy-at-microscopy.com} ; Mon, 3 Apr 2006
} 21:12:02 -0500
} 7, 12 -- Mime-Version: 1.0
} 7, 12 -- X-Sender: (Unverified)
} 7, 12 -- Message-Id: {p06110403c0578663217d-at-[206.69.208.22]}
} 7, 12 -- Date: Mon, 3 Apr 2006 21:12:01 -0500
} 7, 12 -- To: microscopy-at-microscopy.com
} 7, 12 -- From: rra-at-stowers-institute.org (by way of MicroscopyListserver)
} 7, 12 -- Subject: viaWWW: Leica EM-Stain
} 7, 12 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of -
} Headers==============================
}
}
}
}
} ==============================Original Headers==============================
} 25, 30 -- From christopher.hayden-at-novartis.com Tue Apr 4 06:11:07 2006
} 25, 30 -- Received: from mail84.messagelabs.com (mail84.messagelabs.com [195.245.231.99])
} 25, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k34BB6Kf017329
} 25, 30 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 4 Apr 2006 06:11:06 -0500
} 25, 30 -- X-VirusChecked: Checked
} 25, 30 -- X-Env-Sender: christopher.hayden-at-novartis.com
} 25, 30 -- X-Msg-Ref: server-4.tower-84.messagelabs.com!1144149064!19910770!1
} 25, 30 -- X-StarScan-Version: 5.5.9.1; banners=-,-,-
} 25, 30 -- X-Originating-IP: [160.62.1.174]
} 25, 30 -- Received: (qmail 13192 invoked from network); 4 Apr 2006 11:11:05 -0000
} 25, 30 -- Received: from ch2ssaenov01.novartis.com (HELO ch2ssaenov01.novartis.com) (160.62.1.174)
} 25, 30 -- by server-4.tower-84.messagelabs.com with AES256-SHA encrypted SMTP; 4 Apr 2006 11:11:05 -0000
} 25, 30 -- Received: from mtap2.is.chbs ([192.37.33.19])
} 25, 30 -- by ch2ssaenov01.novartis.com (8.13.6/8.13.4) with ESMTP id k34B8vkO027268;
} 25, 30 -- Tue, 4 Apr 2006 13:08:57 +0200
} 25, 30 -- Received: from phchbs-s3025.EU.novartis.net (phchbs-s3025.eu.novartis.net [192.37.31.249])
} 25, 30 -- by mtap2.is.chbs (Switch-3.1.6/Switch-3.1.6) with ESMTP id k34BB3nH9486452;
} 25, 30 -- Tue, 4 Apr 2006 13:11:04 +0200
} 25, 30 -- To: Microscopy-at-msa.microscopy.com
} 25, 30 -- Cc: rra-at-stowers-institute.org
} 25, 30 -- Subject: Re: [Microscopy] viaWWW: Leica EM-Stain
} 25, 30 -- MIME-Version: 1.0
} 25, 30 -- X-Mailer: Lotus Notes Release 5.0.12 February 13, 2003
} 25, 30 -- Message-ID: {OF829E7CC2.942F7902-ON85257146.003C549D-85257146.003D705C-at-EU.novartis.net}
} 25, 30 -- From: christopher.hayden-at-novartis.com
} 25, 30 -- Date: Tue, 4 Apr 2006 07:11:20 -0400
} 25, 30 -- X-MIMETrack: Serialize by Router on CHBSSPH0/PH/Novartis(5012HF433 | October 14, 2003) at
} 25, 30 -- 04.04.2006 13:11:04,
} 25, 30 -- Serialize complete at 04.04.2006 13:11:04
} 25, 30 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of - Headers==============================



--
Dr. Gerd Leitinger

Institut für Zellbiologie, Histologie und Embryologie
Zentrum für Molekulare Medizin
Medizinische Universität Graz
Harrachgasse 21
A-8010 Graz
Austria

Tel. ++43 316 380 4237
Fax. ++43 316 380 9625
Mailto: Gerd.Leitinger-at-meduni-graz.at



==============================Original Headers==============================
13, 20 -- From gerd.leitinger-at-meduni-graz.at Wed Apr 5 05:51:48 2006
13, 20 -- Received: from herakles.kfunigraz.ac.at (HERAKLES.kfunigraz.ac.at [143.50.13.36])
13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k35Aplkv023601
13, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 05:51:47 -0500
13, 20 -- Received: from [10.200.112.73] ([193.170.104.112]) by herakles.kfunigraz.ac.at with Microsoft SMTPSVC(5.0.2195.6713);
13, 20 -- Wed, 5 Apr 2006 12:51:46 +0200
13, 20 -- From: "Gerd Leitinger" {gerd.leitinger-at-meduni-graz.at}
13, 20 -- To: Microscopy-at-microscopy.com
13, 20 -- Date: Wed, 05 Apr 2006 12:50:23 +0200
13, 20 -- MIME-Version: 1.0
13, 20 -- Subject: Re: Leica EM-Stain
13, 20 -- Message-ID: {4433BD0F.24346.F8BE7-at-gerd.leitinger.meduni-graz.at}
13, 20 -- Priority: normal
13, 20 -- In-reply-to: {200604041115.k34BFasV025469-at-ns.microscopy.com}
13, 20 -- X-mailer: Pegasus Mail for Windows (4.31, DE v4.31 R1)
13, 20 -- Content-type: text/plain; charset=ISO-8859-1
13, 20 -- Content-description: Mail message body
13, 20 -- X-OriginalArrivalTime: 05 Apr 2006 10:51:46.0674 (UTC) FILETIME=[EF22A520:01C6589E]
13, 20 -- Content-Transfer-Encoding: 8bit
13, 20 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id k35Aplkv023601
==============================End of - Headers==============================




From: a.d.mckinnon-at-abdn.ac.uk
Date: Wed, 5 Apr 2006 11:55:53 -0500
Subject: [Microscopy] viaWWW: Histology & ElectronMicroscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both a.d.mckinnon-at-abdn.ac.uk as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: a.d.mckinnon-at-abdn.ac.uk
Name: Alastair McKinnon

Organization: University of Aberdeen

Title-Subject: [Filtered] Histology & ElectronMicroscopy

Question: Can anyone suggest an effective algicide that is safe to use in a chiller circulator supplying water at 18-20'C for a Philips CM10 TEM.

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Wed Apr 5 11:55:52 2006
6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k35Gtp2k007755
6, 12 -- for {microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 11:55:52 -0500
6, 12 -- Mime-Version: 1.0
6, 12 -- X-Sender: (Unverified)
6, 12 -- Message-Id: {p0611040ec059a709cb16-at-[206.69.208.22]}
6, 12 -- Date: Wed, 5 Apr 2006 11:55:51 -0500
6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: a.d.mckinnon-at-abdn.ac.uk (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Histology & ElectronMicroscopy
6, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: xiuhuixin-at-yahoo.com.cn
Date: Wed, 5 Apr 2006 11:56:35 -0500
Subject: [Microscopy] viaWWW: Help for FIB prepared specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both xiuhuixin-at-yahoo.com.cn as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: xiuhuixin-at-yahoo.com.cn
Name: Huixin

Title-Subject: [Filtered] Help for FIB prepared specimens

Question: The first problem I met is that sometimes I lost my specimens. The machine I used is a single beam FIB and the material is GaN. After ex-situ lift-out, I put the specimens onto a copper mesh. I used membrane box to store the specimens. However, when I load the specimen into the TEM specimen holder, I cannot find the specimen in the microscope sometimes. Does anybody have a method to prevent specimens from being lost?

The second problem I met is that there is too much surface damage on my specimens. Does anybody have some tips to reduce the surface damage? Thanks all.


---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Wed Apr 5 11:56:35 2006
7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k35GuXa6008346
7, 12 -- for {microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 11:56:34 -0500
7, 12 -- Mime-Version: 1.0
7, 12 -- X-Sender: (Unverified)
7, 12 -- Message-Id: {p0611040fc059a72cd32b-at-[206.69.208.22]}
7, 12 -- Date: Wed, 5 Apr 2006 11:56:33 -0500
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: xiuhuixin-at-yahoo.com.cn (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: Help for FIB prepared specimens
7, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: marie.cheynet-at-ltpcm.inpg.fr
Date: Wed, 5 Apr 2006 11:57:01 -0500
Subject: [Microscopy] viaWWW: polycrystalline alumina cap

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both marie.cheynet-at-ltpcm.inpg.fr as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: marie.cheynet-at-ltpcm.inpg.fr
Name: Cheynet Marie

Organization: CNRS-France

Title-Subject: [Filtered] polycrystalline alumina cap

Question: Bonjour,

I am looking for a solvent to dissolve a 10 nm thick polycrystalline alumina cap deposited on a ultra-thin HfO2 dielectric layer.
Thanks for your suggestions.
marie


---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Wed Apr 5 11:57:01 2006
6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k35Guxx4009498
6, 12 -- for {microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 11:57:01 -0500
6, 12 -- Mime-Version: 1.0
6, 12 -- X-Sender: (Unverified)
6, 12 -- Message-Id: {p06110410c059a748d9ba-at-[206.69.208.22]}
6, 12 -- Date: Wed, 5 Apr 2006 11:56:59 -0500
6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: marie.cheynet-at-ltpcm.inpg.fr (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: polycrystalline alumina cap
6, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: mmiralles-at-pi.ac.ae
Date: Wed, 5 Apr 2006 11:57:32 -0500
Subject: [Microscopy] viaWWW: E-SEM Buy-off Checklist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mmiralles-at-pi.ac.ae as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: mmiralles-at-pi.ac.ae
Name: myleen

Title-Subject: [Filtered] E-SEM Buy-off Checklist

Question: hi,

we will be acquiring an E-SEM very soon (due for delivery this May 2006). just wondering if anyone has an equipment buy-off checklist i could use as a pattern for checking the machine?

thank you so much!

---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Wed Apr 5 11:57:31 2006
7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k35GvU5H011270
7, 12 -- for {microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 11:57:31 -0500
7, 12 -- Mime-Version: 1.0
7, 12 -- X-Sender: (Unverified)
7, 12 -- Message-Id: {p06110411c059a762dfc5-at-[206.69.208.22]}
7, 12 -- Date: Wed, 5 Apr 2006 11:57:29 -0500
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: mmiralles-at-pi.ac.ae (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: E-SEM Buy-off Checklist
7, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Wed, 5 Apr 2006 12:11:39 -0500
Subject: [Microscopy] Re: viaWWW: Histology & ElectronMicroscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Please reply to the list as well -- I have the same instrument, and
the same question.

Phil

} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both a.d.mckinnon-at-abdn.ac.uk as well as the
} MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: a.d.mckinnon-at-abdn.ac.uk
} Name: Alastair McKinnon
}
} Organization: University of Aberdeen
}
} Title-Subject: [Filtered] Histology & ElectronMicroscopy
}
} Question: Can anyone suggest an effective algicide that is safe to
} use in a chiller circulator supplying water at 18-20'C for a Philips
} CM10 TEM.
--
Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
024C Brooks Hall
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
3, 22 -- From oshel1pe-at-cmich.edu Wed Apr 5 12:11:38 2006
3, 22 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21])
3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k35HBcAD013817
3, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 12:11:38 -0500
3, 22 -- Received: from egateb.central.cmich.local ([141.209.15.85])
3, 22 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id k35Hn54l015106
3, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 13:49:05 -0400
3, 22 -- Received: from [141.209.160.132] ([141.209.160.132]) by egateb.central.cmich.local with Microsoft SMTPSVC(5.0.2195.6713);
3, 22 -- Wed, 5 Apr 2006 13:11:36 -0400
3, 22 -- Mime-Version: 1.0
3, 22 -- Message-Id: {f0623090ac059aa9c0b5f-at-[141.209.160.132]}
3, 22 -- In-Reply-To: {200604051705.k35H5f8q006805-at-ns.microscopy.com}
3, 22 -- References: {200604051705.k35H5f8q006805-at-ns.microscopy.com}
3, 22 -- Date: Wed, 5 Apr 2006 13:11:33 -0400
3, 22 -- To: Microscopy-at-microscopy.com
3, 22 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
3, 22 -- Subject: Re: [Microscopy] viaWWW: Histology & ElectronMicroscopy
3, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
3, 22 -- X-OriginalArrivalTime: 05 Apr 2006 17:11:36.0758 (UTC) FILETIME=[FF157D60:01C658D3]
3, 22 -- X-CanItPRO-Stream: default
3, 22 -- X-Spam-Score: -4 () L_EXCH_MF
3, 22 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Wed, 5 Apr 2006 13:09:37 -0500
Subject: [Microscopy] Re: viaWWW: Histology & ElectronMicroscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Apr 5, 2006, at 9:56 AM, a.d.mckinnon-at-abdn.ac.uk wrote:

} Question: Can anyone suggest an effective algicide that is safe to use
} in a chiller circulator supplying water at 18-20'C for a Philips CM10
} TEM.
}
Dear Alastair,
We sprinkle a small amount of
2,2'-Methylenebis(4-chlorophenol)--dichlorophene for short--onto the
surface of the chiller water. It is not very soluble, so it floats on
the surface in clumps. When we don't see any more clumps, we add a
little more dichlorophene. I also used this compound in the chillers
on the HVEM in Albany NY for more than 20 years, and there was no
observable harm to either the chillers or the scope; I measured the
water flow rates through the lenses annually, and there was essentially
no change over the entire time.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
4, 22 -- From tivol-at-caltech.edu Wed Apr 5 13:09:36 2006
4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k35I9a0Y024296
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 5 Apr 2006 13:09:36 -0500
4, 22 -- Received: from localhost (fire-dog [192.168.1.4])
4, 22 -- by water-ox-postvirus (Postfix) with ESMTP
4, 22 -- id 7E40B3496E; Wed, 5 Apr 2006 11:09:35 -0700 (PDT)
4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21])
4, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP
4, 22 -- id 2F7EF34A05; Wed, 5 Apr 2006 11:09:34 -0700 (PDT)
4, 22 -- Mime-Version: 1.0 (Apple Message framework v623)
4, 22 -- In-Reply-To: {200604051656.k35Gu7ik007931-at-ns.microscopy.com}
4, 22 -- References: {200604051656.k35Gu7ik007931-at-ns.microscopy.com}
4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
4, 22 -- Message-Id: {ea030c84e0b3f694978483884f24db9d-at-caltech.edu}
4, 22 -- Content-Transfer-Encoding: 7bit
4, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
4, 22 -- Subject: Re: [Microscopy] viaWWW: Histology & ElectronMicroscopy
4, 22 -- Date: Wed, 5 Apr 2006 11:19:02 -0700
4, 22 -- To: microscopy-at-msa.microscopy.com, a.d.mckinnon-at-abdn.ac.uk
4, 22 -- X-Mailer: Apple Mail (2.623)
4, 22 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.3.3
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Wed, 5 Apr 2006 13:10:17 -0500
Subject: [Microscopy] LM: flouresc cw stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

A colleage is looking for a red fluorescent dye to stain lignin (or
plant cell wall) for viewing in a confocal microscope. Any
suggestions would be appreciated.

Thank you.

JB
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################

==============================Original Headers==============================
3, 16 -- From bozzola-at-siu.edu Wed Apr 5 13:10:17 2006
3, 16 -- Received: from abbmta2.siu.edu (abbmta2.siu.edu [131.230.254.206])
3, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k35IAHpR025376
3, 16 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 5 Apr 2006 13:10:17 -0500
3, 16 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142])
3, 16 -- by abbmta2.siu.edu (Switch-3.1.8/Switch-3.1.6) with ESMTP id k35IAHpT011183
3, 16 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 5 Apr 2006 13:10:17 -0500 (CDT)
3, 16 -- Mime-Version: 1.0
3, 16 -- X-Sender: bozzola-at-saluki-mail.siu.edu
3, 16 -- Message-Id: {p06110408c059b8174ef1-at-[131.230.177.142]}
3, 16 -- Date: Wed, 5 Apr 2006 13:10:15 -0500
3, 16 -- To: Microscopy-at-msa.microscopy.com
3, 16 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
3, 16 -- Subject: LM: flouresc cw stain
3, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
3, 16 -- X-MASF: 0.00%
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Wed, 5 Apr 2006 13:12:00 -0500
Subject: [Microscopy] Re: viaWWW: polycrystalline alumina cap

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Apr 5, 2006, at 9:57 AM, marie.cheynet-at-ltpcm.inpg.fr wrote:

} I am looking for a solvent to dissolve a 10 nm thick polycrystalline
} alumina cap deposited on a ultra-thin HfO2 dielectric layer.
} Thanks for your suggestions.
}
Dear Marie,
I do not know what the effect would be on the HfO2, but Al2O3 will
dissolve in strong base.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
4, 22 -- From tivol-at-caltech.edu Wed Apr 5 13:12:00 2006
4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k35IBxMb030897
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 5 Apr 2006 13:12:00 -0500
4, 22 -- Received: from localhost (fire-dog [192.168.1.4])
4, 22 -- by earth-ox-postvirus (Postfix) with ESMTP
4, 22 -- id A5C0B10ACB5; Wed, 5 Apr 2006 11:11:59 -0700 (PDT)
4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21])
4, 22 -- by earth-ox.its.caltech.edu (Postfix) with ESMTP
4, 22 -- id B9D0810AC49; Wed, 5 Apr 2006 11:11:58 -0700 (PDT)
4, 22 -- Mime-Version: 1.0 (Apple Message framework v623)
4, 22 -- In-Reply-To: {200604051657.k35Gv9OF009943-at-ns.microscopy.com}
4, 22 -- References: {200604051657.k35Gv9OF009943-at-ns.microscopy.com}
4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
4, 22 -- Message-Id: {7e0081e0eed22f808166d15dd8fd0c14-at-caltech.edu}
4, 22 -- Content-Transfer-Encoding: 7bit
4, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
4, 22 -- Subject: Re: [Microscopy] viaWWW: polycrystalline alumina cap
4, 22 -- Date: Wed, 5 Apr 2006 11:21:25 -0700
4, 22 -- To: marie.cheynet-at-ltpcm.inpg.fr, microscopy-at-msa.microscopy.com
4, 22 -- X-Mailer: Apple Mail (2.623)
4, 22 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.3.3
==============================End of - Headers==============================




From: rhberg-at-danforthcenter.org
Date: Wed, 5 Apr 2006 14:14:55 -0500
Subject: [Microscopy] Re: LM: flouresc cw stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

John,

Congo Red binds cellulose and would therefore be a good fluorescence
stain for plant cell walls emitting in the red (emission~ 590 nm).
As for a fluorescent lignin stain, keep in mind that lignin is
autofluorescent (broadband emission ~470-520 nm). It could be
stained with a fluorescent Schiff's base (e.g., Auramine O) as well.

Hope this helps.

Howard


On Apr 5, 2006, at 1:11 PM, bozzola-at-siu.edu wrote:

}
}
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/
} MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} A colleage is looking for a red fluorescent dye to stain lignin (or
} plant cell wall) for viewing in a confocal microscope. Any
} suggestions would be appreciated.
}
} Thank you.
}
} JB
} --
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/~image/
} ##############################################################
}
} ==============================Original
} Headers==============================
} 3, 16 -- From bozzola-at-siu.edu Wed Apr 5 13:10:17 2006
} 3, 16 -- Received: from abbmta2.siu.edu (abbmta2.siu.edu
} [131.230.254.206])
} 3, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id k35IAHpR025376
} 3, 16 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 5 Apr 2006
} 13:10:17 -0500
} 3, 16 -- Received: from [131.230.177.142]
} (ws177142.microscope.siu.edu [131.230.177.142])
} 3, 16 -- by abbmta2.siu.edu (Switch-3.1.8/Switch-3.1.6) with ESMTP
} id k35IAHpT011183
} 3, 16 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 5 Apr 2006
} 13:10:17 -0500 (CDT)
} 3, 16 -- Mime-Version: 1.0
} 3, 16 -- X-Sender: bozzola-at-saluki-mail.siu.edu
} 3, 16 -- Message-Id: {p06110408c059b8174ef1-at-[131.230.177.142]}
} 3, 16 -- Date: Wed, 5 Apr 2006 13:10:15 -0500
} 3, 16 -- To: Microscopy-at-msa.microscopy.com
} 3, 16 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
} 3, 16 -- Subject: LM: flouresc cw stain
} 3, 16 -- Content-Type: text/plain; charset="us-ascii" ;
} format="flowed"
} 3, 16 -- X-MASF: 0.00%
} ==============================End of -
} Headers==============================



R. Howard Berg, Ph.D.
Director, Integrated Microscopy Facility
Danforth Plant Science Center
975 N. Warson Rd.
St. Louis, MO 63132

ph 314-587-1261 fx 314-587-1361 cell 314-378-2409
rhberg-at-danforthcenter.org www.danforthcenter.org
visit this educational resource: http://www.danforthcenter.org/Cells/


==============================Original Headers==============================
12, 23 -- From RHBerg-at-danforthcenter.org Wed Apr 5 14:14:55 2006
12, 23 -- Received: from spm1.ddpsc.org (spm1.danforthcenter.org [65.254.111.26])
12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k35JEs5w021491
12, 23 -- for {microscopy-at-sparc5.microscopy.com} ; Wed, 5 Apr 2006 14:14:54 -0500
12, 23 -- Received: from spm1.ddpsc.org (127.0.0.1) by spm1.ddpsc.org (MlfMTA v3.1r24) id h6gbis0171sp for {microscopy-at-sparc5.microscopy.com} ; Wed, 5 Apr 2006 14:14:53 -0500 (envelope-from {RHBerg-at-danforthcenter.org} )
12, 23 -- Received: from mail02.ddpsc.org ([10.101.0.23])
12, 23 -- by spm1.ddpsc.org (MailFrontier 4.5.7.7473)
12, 23 -- with ESMTP; Wed, 05 Apr 2006 14:14:53 -0500
12, 23 -- Received: from [10.14.0.20] ([10.14.0.20] unverified) by mail02.ddpsc.org with Microsoft SMTPSVC(5.0.2195.6713);
12, 23 -- Wed, 5 Apr 2006 14:14:53 -0500
12, 23 -- Mime-Version: 1.0 (Apple Message framework v746.3)
12, 23 -- In-Reply-To: {200604051811.k35IBiYY030143-at-ns.microscopy.com}
12, 23 -- References: {200604051811.k35IBiYY030143-at-ns.microscopy.com}
12, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
12, 23 -- Message-Id: {6DB37DB7-A5C8-4D9D-BED7-62E6916A3907-at-danforthcenter.org}
12, 23 -- Content-Transfer-Encoding: 7bit
12, 23 -- From: "R. Howard Berg" {rhberg-at-danforthcenter.org}
12, 23 -- Subject: Re: [Microscopy] LM: flouresc cw stain
12, 23 -- Date: Wed, 5 Apr 2006 14:14:50 -0500
12, 23 -- To: microscopy-at-ns.microscopy.com
12, 23 -- X-Mailer: Apple Mail (2.746.3)
12, 23 -- X-OriginalArrivalTime: 05 Apr 2006 19:14:53.0007 (UTC) FILETIME=[379785F0:01C658E5]
12, 23 -- X-Mlf-Version: 4.5.7.7473
==============================End of - Headers==============================




From: bigelow-at-engin.umich.edu
Date: Wed, 5 Apr 2006 14:55:40 -0500
Subject: [Microscopy] Specimen protection fro atmosphere

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Concerning Gary's inquiry about a small vacuum chamber for specimen transfer.

I have produced specimen rods for two models of TEMs that give
protection of a specimen from the atmosphere for a brief period while
being moved from a reaction chamber into the microscope specimen
stage, and also a small glass reaction chamber for use with such
holders - if such might be of interest in this type of application.
The glass reactor is small enough to be used for the kind of
operation mentioned.
--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-engin.umich.edu;
Fx:734-763-4788; Ph:734-662-5237
Address mail to: 1136 Mixtwood Rd.
Ann Arbor, MI 48103-3035

==============================Original Headers==============================
2, 14 -- From bigelow-at-engin.umich.edu Wed Apr 5 14:55:39 2006
2, 14 -- Received: from srvr22.engin.umich.edu (srvr22.engin.umich.edu [141.213.75.21])
2, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k35JtdTb031483
2, 14 -- for {microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 14:55:39 -0500
2, 14 -- Received: from [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221])
2, 14 -- by srvr22.engin.umich.edu (8.13.6/8.13.5) with ESMTP id k35JtcGY026563
2, 14 -- for {microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 15:55:38 -0400 (EDT)
2, 14 -- Mime-Version: 1.0
2, 14 -- Message-Id: {p06210202c059cf72df14-at-[141.212.131.221]}
2, 14 -- Date: Wed, 5 Apr 2006 15:55:37 -0400
2, 14 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com}
2, 14 -- From: Wil Bigelow {bigelow-at-engin.umich.edu}
2, 14 -- Subject: [Microscopy] Specimen protection fro atmosphere
2, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: avklaus-at-amnh.org
Date: Wed, 5 Apr 2006 15:48:54 -0500
Subject: [Microscopy] TEM protocol: male gametes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

Would anyone have a detailed protocol for preparing mouse sp*rm suspensions
for TEM that they would be willing to share? I've been attempting to
concentrate my sample by centrifugation, then resuspend in agar, but I'm not
getting very good results.

Many thanks in advance for any suggestions, comments, or protocols.

(Note: I seem to be having some trouble posting due to my subject matter.
Please forgive the work-around!)

Best regards,

Angela

--
Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West and 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-796-5977
Fax: 212-496-3480


==============================Original Headers==============================
8, 29 -- From avklaus-at-amnh.org Wed Apr 5 15:48:54 2006
8, 29 -- Received: from lepore.amnh.org (lepore.amnh.org [216.73.241.12])
8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k35KmsTM009271
8, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 15:48:54 -0500
8, 29 -- Received: from localhost (localhost [127.0.0.1])
8, 29 -- by lepore.amnh.org (Postfix) with ESMTP id D237F5B7A1;
8, 29 -- Wed, 5 Apr 2006 16:48:53 -0400 (EDT)
8, 29 -- Received: from lepore.amnh.org ([127.0.0.1])
8, 29 -- by localhost (lepore.amnh.org [127.0.0.1]) (amavisd-new, port 10024)
8, 29 -- with ESMTP id 01224-04; Wed, 5 Apr 2006 16:48:52 -0400 (EDT)
8, 29 -- Received: from webmail.amnh.org (cain.amnh.org [216.73.241.17])
8, 29 -- by lepore.amnh.org (Postfix) with ESMTP id C760B5B795;
8, 29 -- Wed, 5 Apr 2006 16:48:52 -0400 (EDT)
8, 29 -- Received: from 216.73.250.195
8, 29 -- (SquirrelMail authenticated user avklaus)
8, 29 -- by webmail.amnh.org with HTTP;
8, 29 -- Wed, 5 Apr 2006 16:48:52 -0400 (EDT)
8, 29 -- Message-ID: {2020.216.73.250.195.1144270132.squirrel-at-webmail.amnh.org}
8, 29 -- Date: Wed, 5 Apr 2006 16:48:52 -0400 (EDT)
8, 29 -- Subject: TEM protocol: male gametes
8, 29 -- From: "Angela V. Klaus" {avklaus-at-amnh.org}
8, 29 -- To: Microscopy-at-microscopy.com
8, 29 -- User-Agent: SquirrelMail/1.4.4
8, 29 -- MIME-Version: 1.0
8, 29 -- Content-Type: text/plain;charset=iso-8859-1
8, 29 -- Content-Transfer-Encoding: 8bit
8, 29 -- X-Priority: 3 (Normal)
8, 29 -- Importance: Normal
8, 29 -- X-Virus-Scanned: amavisd-new at amnh.org
==============================End of - Headers==============================




From: CavinM-at-vsl.cua.edu
Date: Wed, 5 Apr 2006 16:30:08 -0500
Subject: [Microscopy] Position available: Electron Microscopist/Lab Manager

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Electron Microscopist / Lab Manager
The Catholic University of America,
Vitreous State Laboratory, Washington, D.C.

Appointment Date/Term: Immediate/permanent

Salary and Benefits: Negotiable, 2x matching 403b, (up to 5% of salary after 1 year,) 21 days paid vacation, 15 days paid holiday, sick leave, health and life insurance, flexible spending account for medical/dental reimbursement, tuition reimbursement, (up to 6 credits per semester,) and relocation assistance negotiable.

Essential Duties: Microstructural characterization of materials utilizing OLM, SEM, EDS, WDS, TEM, STEM, and XRD. Provide technical expertise to researchers on equipment operation and microscopy laboratory protocols. Conduct basic EM maintenance on related equipment.

Qualifications: Candidate must either have a two-year degree in electron microscopy or a BS in physics, chemistry, or materials science with 3-5 years of hands-on electron microscopy experience. Candidate must have detailed operational knowledge of scanning and transmission electron microscopes, as well as, energy-dispersive spectroscopy systems, fundamental understanding of image processing and analysis techniques, capacity to prepare SEM specimens utilizing standard metallographic techniques, ability to prepare TEM specimens via tripod polishing, jet polishing, dimpling/ion milling, and extraction replication, a generally good understanding of electron microscopy lab maintenance, and strong verbal and written communication skills. EM lab management experience a major plus.

Instrumentation: JEOL 5900LV SEM with Oxford INCA ENERGY 300/WAVE 700 EDS/WDS systems, JEOL 35c SEM with two JEOL FCS WD-spectrometers interfaced to Noran Vantage EDS system, JEOL 2000EX / FX TEM/STEM w/Oxford INCA-ISIS ENERGY 200 EDS, Olympus upright light microscope w/ brightfield transmitted and reflected/polarizing light, Leica inverted stage w/ brightfield, darkfield, polarizing, Nomarski DIC.

Cavin T. F. Mooers
EM Facility Manager
The Catholic University of America
Vitreous State Laboratory
Hannan Hall Rm 433
Washington, D.C. 20064
(202) 319-6237 (Office)
(202) 319-5346 (Lab)
(202) 319-4469 (Fax)


Send resume and cover letter with salary requirements via email reply.



______________ ______________ ______________ ______________
Sent via VSL Webmail





==============================Original Headers==============================
12, 19 -- From CavinM-at-vsl.cua.edu Wed Apr 5 16:30:08 2006
12, 19 -- Received: from hermes.vsl.cua.edu (interface.vsl.cua.edu [136.242.188.2])
12, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k35LU4ZW019652
12, 19 -- for {microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 16:30:05 -0500
12, 19 -- X-Filtered-With-Copfilter: Version 0.82 (ProxSMTP 1.3.91)
12, 19 -- X-Copfilter-Virus-Scanned: ClamAV 0.88/1376 - Wed Apr 5 00:51:25 2006
12, 19 -- X-Copfilter: Client is part of our network, skipped SpamAssassin
12, 19 -- Received: from 136.242.189.141 with HTTP
12, 19 -- by webserver hermes.vsl.cua.edu (136.242.189.3) ; Wed, 5 Apr 2006 17:30:03 EDT
12, 19 -- Date: Wed, 5 Apr 2006 17:30:04 -0400
12, 19 -- Message-Id: {200604051730.AA171114822-at-hermes.vsl.cua.edu}
12, 19 -- Mime-Version: 1.0
12, 19 -- Content-Type: text/plain; charset=us-ascii
12, 19 -- From: "Cavin Mooers" {CavinM-at-vsl.cua.edu}
12, 19 -- Reply-To: {CavinM-at-vsl.cua.edu}
12, 19 -- X-Sender: {CavinM-at-vsl.cua.edu}
12, 19 -- To: {microscopy-at-microscopy.com}
12, 19 -- Subject: Position available: Electron Microscopist/Lab Manager
12, 19 -- X-Mailer: {IMail v8.21}
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Wed, 5 Apr 2006 19:23:51 -0500
Subject: [Microscopy] Re: Small vacuum box

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to all for the suggestions. I suppose that
I should have provided more info about what I seek.

I am looking for a small vacuum container that would hold
specimens in storage containers like Pella 16708 which
have plastic specimen locations like Pella 16166.

The plastic box is 3-11/16 x 2-11/16 x 1-1/2" and will
not fit in a KF25 pipe. So I need some little dessicator
unit that is easily transportable. The issue is getting
the specimen out of RIE and/or FIB tool over to EBSD tool
without growing too much oxide such that EBSD does not pattern.

gary g.


At 04:21 PM 4/4/2006, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
10, 19 -- From gary-at-gaugler.com Wed Apr 5 19:23:51 2006
10, 19 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145])
10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k360NouG031623
10, 19 -- for {microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 19:23:50 -0500
10, 19 -- Received: (qmail 26997 invoked from network); 5 Apr 2006 17:23:50 -0700
10, 19 -- Received: by simscan 1.1.0 ppid: 26994, pid: 26995, t: 0.1701s
10, 19 -- scanners: regex: 1.1.0 attach: 1.1.0
10, 19 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211)
10, 19 -- by qsmtp3 with SMTP; 5 Apr 2006 17:23:49 -0700
10, 19 -- Message-Id: {7.0.1.0.2.20060405171807.023852c0-at-gaugler.com}
10, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0
10, 19 -- Date: Wed, 05 Apr 2006 17:23:51 -0700
10, 19 -- To: MSA listserver {microscopy-at-microscopy.com}
10, 19 -- From: Gary Gaugler {gary-at-gaugler.com}
10, 19 -- Subject: Re: [Microscopy] Small vacuum box
10, 19 -- In-Reply-To: {200604042321.k34NLsTG020664-at-ns.microscopy.com}
10, 19 -- References: {200604042321.k34NLsTG020664-at-ns.microscopy.com}
10, 19 -- Mime-Version: 1.0
10, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: walck-at-southbaytech.com
Date: Wed, 5 Apr 2006 19:43:48 -0500
Subject: [Microscopy] viaWWW: Help for FIB prepared specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

FIB Lift-Out Protection:
At the MRS meeting in San Francisco (April 17-21), we are introducing
the large size SampleSaver(TM) Storage Container (model# SS-200) along
with FIB specimen holders with CastleGuard(TM) protection. These
holders are designed to hold a cut grid or an Omniprobe(R) grid in the
upright position for the in-situ lift-out process in the FIB. After
removal from the FIB, it can be stored in a numbered insert for the
SS-200 and held in place with a set screw so that the SampleSaver(TM)
unit can be transported. I have placed OmniProbe grids into these
holders and dropped them repeatedly from a height of six feet without
any of them falling out. The CastleGuard protection prevents damage to
the sample even if dropped to the floor head-first, i.e. specimen side
down. We are about to send some samples around the world to Hungary to
see if they can survive FedEx international handling. (Anybody want to
take bets?) If you are interested in this sample holder, I can send an
image if you respond to me offline.

FIB Damage:
To remove the surface damage from FIB samples, you need to ion mill at
low energies at low angles. We sell the Gentle Mill which was
specifically designed to remove the surface layer from FIB samples and
conventional ion milling samples for high resolution applications. Our
IV3/IV4 ion mill system can also be configured with a low energy gun.
This gun, designed and patented by Arpa Barna and associates, can
operate at an accelerating voltage from as low as 100 eV up to 2 kV. It
uses an electron source to increase ionization of the gas and an Einzel
lens to focus the ions at the sample to increase ion current density.
At 2 kV, this gun can rival the thinning rates of other guns operating
around 5 kV. If you visit our application notes section of our website,
we have an application note entitled, "Applications of the
GentleMill(TM) To FIB Prepared TEM Samples" that shows what it can do on
an FIB prepared TEM sample. Barna and Pecz (A. Barna and B. Pecz,
Ultramicroscopy 70, 1998) have shown that conventional ion milling with
3 keV Ar ions can give an amorphous layer of as much as 120 A on each
ion milled surface, but with 250 eV the layer is only about 10 A. I can
also send you the data for that paper if you contact me offline.


Disclaimer: We did not "Plant" these questions!! South Bay Technology,
Inc. manufactures and sells the SampleSaver(TM) storage container and
the FIB specimen holder with CastleGuard(TM) protection and sells the
Technoorg-Linde Gentle Mill(TM) and IV3/IV4 ion mills.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: xiuhuixin-at-yahoo.com.cn [mailto:xiuhuixin-at-yahoo.com.cn]
Sent: Wednesday, April 05, 2006 10:04 AM
To: Walck-at-SouthBayTech.com

This Question/Comment was submitted to the Microscopy Listserver using
the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both xiuhuixin-at-yahoo.com.cn as well as the MIcroscopy
Listserver
------------------------------------------------------------------------
---

Email: xiuhuixin-at-yahoo.com.cn
Name: Huixin

Title-Subject: [Filtered] Help for FIB prepared specimens

Question: The first problem I met is that sometimes I lost my specimens.
The machine I used is a single beam FIB and the material is GaN. After
ex-situ lift-out, I put the specimens onto a copper mesh. I used
membrane box to store the specimens. However, when I load the specimen
into the TEM specimen holder, I cannot find the specimen in the
microscope sometimes. Does anybody have a method to prevent specimens
from being lost?

The second problem I met is that there is too much surface damage on my
specimens. Does anybody have some tips to reduce the surface damage?
Thanks all.


------------------------------------------------------------------------
---

==============================Original
Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Wed Apr 5 11:56:35 2006 7, 12 --
Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id k35GuXa6008346
7, 12 -- for {microscopy-at-microscopy.com} ; Wed, 5 Apr 2006
11:56:34 -0500
7, 12 -- Mime-Version: 1.0
7, 12 -- X-Sender: (Unverified)
7, 12 -- Message-Id: {p0611040fc059a72cd32b-at-[206.69.208.22]}
7, 12 -- Date: Wed, 5 Apr 2006 11:56:33 -0500
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: xiuhuixin-at-yahoo.com.cn (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: Help for FIB prepared specimens 7, 12 --
Content-Type: text/plain; charset="us-ascii"
==============================End of -
Headers==============================


==============================Original Headers==============================
20, 27 -- From walck-at-southbaytech.com Wed Apr 5 19:43:47 2006
20, 27 -- Received: from ylpvm01.prodigy.net (ylpvm01-ext.prodigy.net [207.115.57.32])
20, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k360hlCB010418
20, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 19:43:47 -0500
20, 27 -- Received: from pimout5-ext.prodigy.net (pimout5-int.prodigy.net [207.115.4.21])
20, 27 -- by ylpvm01.prodigy.net (8.12.10 outbound/8.12.10) with ESMTP id k360hiag008115
20, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 20:43:44 -0400
20, 27 -- X-ORBL: [64.169.193.90]
20, 27 -- Received: from dynamicbl8uno3 (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90])
20, 27 -- by pimout5-ext.prodigy.net (8.13.4 outbound domainkey aix/8.13.4) with ESMTP id k360hfex087854;
20, 27 -- Wed, 5 Apr 2006 20:43:46 -0400
20, 27 -- From: "Scott Walck" {walck-at-southbaytech.com}
20, 27 -- To: {xiuhuixin-at-yahoo.com.cn}
20, 27 -- Cc: {Microscopy-at-microscopy.com}
20, 27 -- Subject: RE: [Microscopy] viaWWW: Help for FIB prepared specimens
20, 27 -- Date: Wed, 5 Apr 2006 17:43:52 -0700
20, 27 -- Message-ID: {009401c65913$30627f50$7801a8c0-at-dynamicbl8uno3}
20, 27 -- MIME-Version: 1.0
20, 27 -- Content-Type: text/plain;
20, 27 -- charset="us-ascii"
20, 27 -- Content-Transfer-Encoding: 7bit
20, 27 -- X-Priority: 3 (Normal)
20, 27 -- X-MSMail-Priority: Normal
20, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.2627
20, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
20, 27 -- In-Reply-To: {200604051703.k35H3f8L000752-at-ns.microscopy.com}
20, 27 -- Importance: Normal
==============================End of - Headers==============================




From: clei-at-uiuc.edu
Date: Wed, 5 Apr 2006 21:32:47 -0500
Subject: [Microscopy] Re: viaWWW: help for MgO prepration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I do not think it hard to prepare the cross-sectional MgO
samples. What you need is the patience. Gatan kits are very
convenient!

1) Prepare the sandwitch, and glue the slabs face-to-face.
The M-bond is good enough, but G-1 epoxy from Gatan is
better for MgO samples.

2) You should polish the 1st side very well. I usually use
the 5micron sand paper before I polish the sandwitch with 1
micron diamond paste. Just use the big polishing machine
which can be found almost each metallurgy lab. Since MgO is
very easy to be polished, you can directly go to 1 micron
after the grinding with sand paper. Please check with light
microscope at 200x, you should find no scratches.

3) After you turn to 2nd side, first grind the sandwitch to
about 100-80 microns. 5-micron sand paper should be the used
finally. Then you can mount the samples on dimpler, and
dimpler the disk down to 35 microns (3-micron diamond
paste). You may see some small scratches at the center due
to the dimpling with metal wheels.

4) The you can polish the samples down to 15 microns thick
on the dimpler machines. I usually use the large force and
high speed. First try 5 or 3 microns diamond paste, and then
1 micron finally. It usually takes more than half hour to
get the good samples. But it deserves!

5) when you glue the disk on the Cu-grid, you can either
glue the grid with 1 side or 2 side. I prefer to the 1 side,
as you do not need adjust the eucentric position much in TEM.
But you really need skills and practice.

6) I use the Gatan Dual ot Fishion miller to ion mill the
samples. Gatan pips works very faster. But use a little low
voltage and current.

Hope these words help you.

Changhui




---- Original message ----
} Date: Mon, 3 Apr 2006 21:13:39 -0500
} From: chao.wang-at-materials.ox.ac.uk
} Subject: [Microscopy] viaWWW: help for MgO prepration and
Fe oxidation
} To: clei-at-uiuc.edu
}
}
}
}
} ------------------------------------------------------------
----------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
Society of America


==============================Original Headers==============================
12, 27 -- From clei-at-uiuc.edu Wed Apr 5 21:32:46 2006
12, 27 -- Received: from expredir5.cites.uiuc.edu (expredir5.cites.uiuc.edu [128.174.5.96])
12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k362WkOr021892
12, 27 -- for {microscopy-at-microscopy.com} ; Wed, 5 Apr 2006 21:32:46 -0500
12, 27 -- Received: from expms6.cites.uiuc.edu (expms6.cites.uiuc.edu [128.174.5.43])
12, 27 -- by expredir5.cites.uiuc.edu (8.13.6/8.13.6) with ESMTP id k362WdCe019013;
12, 27 -- Wed, 5 Apr 2006 21:32:44 -0500 (CDT)
12, 27 -- Received: from expms6.cites.uiuc.edu (localhost.cites.uiuc.edu [127.0.0.1])
12, 27 -- by expms6.cites.uiuc.edu (MOS 3.4.8-GR)
12, 27 -- with ESMTP id BHB81957;
12, 27 -- Wed, 5 Apr 2006 21:32:33 -0500 (CDT)
12, 27 -- Received: from 128.174.5.212
12, 27 -- by expms6.cites.uiuc.edu (MOS 3.4.8-GR)
12, 27 -- with HTTP/1.1;
12, 27 -- Wed, 5 Apr 2006 20:32:33 -0600
12, 27 -- Date: Wed, 5 Apr 2006 20:32:33 -0600
12, 27 -- From: Changhui LEI {clei-at-uiuc.edu}
12, 27 -- Subject: Re: [Microscopy] viaWWW: help for MgO prepration
12, 27 -- and Fe oxidation
12, 27 -- To: chao.wang-at-materials.ox.ac.uk
12, 27 -- Cc: microscopy-at-microscopy.com
12, 27 -- Reply-To: clei-at-uiuc.edu
12, 27 -- X-Mailer: Webmail Mirapoint Direct 3.4.8-GR
12, 27 -- MIME-Version: 1.0
12, 27 -- Message-Id: {f27b5254.a4811668.8198300-at-expms6.cites.uiuc.edu}
12, 27 -- Content-Type: text/plain; charset=us-ascii
12, 27 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: gsosinsky-at-ucsd.edu
Date: Thu, 6 Apr 2006 17:34:01 -0500
Subject: [Microscopy] viaWWW: 4th International Congress on Electron Tomography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both gsosinsky-at-ucsd.edu as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: gsosinsky-at-ucsd.edu
Name: Gina Sosinsky

Organization: University of California, San Diego

Title-Subject: [Filtered] 4th International Congress on Electron Tomography

Question: *** On-line Registration and Abstract submission are now open for the 4th International Congress on Electron Tomography (4ICET) to be held Nov. 5-8, 2006 at Paradise Point Resort, San Diego, Ca. ***

Deadline for early registration September 1
Deadline for late registration October 15
Deadline for abstract submission June 15, 2006

Note: Rooms at Paradise Point are on a first come / first served basis.

Please forward this email to others.

===========
4ICET

This congress brings together biologists, biophysicists, computer
scientists, mathematicians, materials scientists and electron
optical instrumentation specialists for an interdisciplinary exchange of
ideas focusing on advancing methods of electron
tomography (ET) in biology. ET has moved from a specialized experimental
technique practiced by a few laboratories to one delivering critical
information to a broad community of cell biologists, structural
biologists, and neuroscientists. For students, this conference presents
a unique opportunity to learn about cutting-edge advances in ET
applications and methodologies.

Sessions will include:

ï Imaging of dynamic structures and correlative microscopy
Co-Chairs: O. Medalia (Ben-Gurion Univ.); Jack Johnson (The Scripps
Research Institute)

ï Advances in instrumentation
Co-chairs: M. Ellisman (UCSD); Bram Koster (Leiden University Medical Center, Netherlands)

ï 3D reconstruction algorithms
Co-chairs: N. Volkmann (Burnham Institute); Michael Rademacher (Univ. of Vermont)

ï Visualization & quantitative analysis
Co-chairs: R. Whitaker (Univ. of Utah); D. Mastronarde (Univ. of Colorado)

ï Moving tomography to the mainstream: data sharing, data integration, &
model building
Co-chairs: M. Martone (UCSD); J-M. Carazo (Univ. of Madrid)

ï Emerging technologies for the multiscale: cell to tissue and molecule
to cell
Co-chairs: D. Hanein (Burnham Institute); C. Larabell (UCSF)

Sessions will include both invited speakers and talks selected from
submitted abstracts.

For further information please check out our web site:
http://www.4icet.org or contact Mark H. Ellisman, Lead Congress
Organizer (mark-at-ncmir.ucsd.edu) or Grace Osborne, Congress Coordinator
(gosborne-at-ncmir,ucsd.edu)



---------------------------------------------------------------------------


==============================Original Headers==============================
23, 14 -- From zaluzec-at-microscopy.com Thu Apr 6 17:34:01 2006
23, 14 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
23, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k36MXxtq016564
23, 14 -- for {microscopy-at-microscopy.com} ; Thu, 6 Apr 2006 17:34:00 -0500
23, 14 -- Mime-Version: 1.0
23, 14 -- X-Sender: (Unverified)
23, 14 -- Message-Id: {p06110402c05b47986e5e-at-[206.69.208.22]}
23, 14 -- Date: Thu, 6 Apr 2006 17:33:58 -0500
23, 14 -- To: microscopy-at-microscopy.com
23, 14 -- From: gsosinsky-at-ucsd.edu (by way of MicroscopyListserver)
23, 14 -- Subject: viaWWW: 4th International Congress on Electron Tomography
23, 14 -- Content-Type: text/plain; charset="iso-8859-1"
23, 14 -- Content-Transfer-Encoding: 8bit
23, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k36MXxtq016564
==============================End of - Headers==============================




From: temanalysis-at-yahoo.com
Date: Thu, 6 Apr 2006 17:34:53 -0500
Subject: [Microscopy] viaWWW: TEM/FIB: FEI 200 single beam FIB question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both temanalysis-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: temanalysis-at-yahoo.com
Name: Sandra Keller

Organization: Independant Lab

Title-Subject: [Filtered] TEM/FIB: FEI 200 single beam FIB question

Question: Hi:
We are thinking of working a group that wants to add FIB capabilities to their lab to prepare semiconductor samples. They are looking into purchasing a pre-owned FEI 200 (single beam) with a Magnum column.

I have talked to a few of my contacts and they believe this instrument would be best suited to prepare TEM samples of larger technologies at best. They believe something like a pre-owned dual beam FEI 820 or similar might be a better choice for general TEM sample preparation. I don't really know the answer, but wanted to get actual users opninions.

Ideally, I would like to receive feedback of actual users of the FEI 200 with the Magnum column and see if they can easily prepare semiconductor TEM samples with smaller technologies and:
1) Approximately how long it takes to make a sample
2) What size geometries they can easily and routinely section.

If you do not use this particular instrument, but are a frequent FIB user and have a general opinion, I would like to hear from you also.

Thanks for being a great information resource,
Sandra Keller


---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Thu Apr 6 17:34:53 2006
7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k36MYp0S017153
7, 12 -- for {microscopy-at-microscopy.com} ; Thu, 6 Apr 2006 17:34:52 -0500
7, 12 -- Mime-Version: 1.0
7, 12 -- X-Sender: (Unverified)
7, 12 -- Message-Id: {p06110403c05b47dc7e50-at-[206.69.208.22]}
7, 12 -- Date: Thu, 6 Apr 2006 17:34:51 -0500
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: temanalysis-at-yahoo.com (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: TEM/FIB: FEI 200 single beam FIB question
7, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Fri, 7 Apr 2006 03:54:20 -0500
Subject: [Microscopy] Zeiss Axiovert 200M: objectives and axiovision questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,

I am currently using our Zeiss axiovert 200M to
observe cell comparments (mitochondria, golgi,
endosomes,...) by fluorescence.
For this purpose, the main "usable" objectives
available are:
- 40x/0,50 LD A-Plan
- 100x/1,3 Oil EC Plan Neofluar

The 40x LD is useful for our life cell imaging
experiments, so I don't think we should change it.
But I wonder if the 100x is really the best solution.
I must say that I am a little bit disappointed by the
quality of my images (I also try to improve the
preparation of the samples!). When i look to the
"images samples" on the CD which was included with the
axiovision soft, I notice that most of the pictures of
cells are taken with a 63x apochromat.
I searched the site of Zeiss and found an interesting
63x apochromat with a NA of 1.4 and corrected for
coverglass thickness (0,17).
What is your experience with the different Zeiss
objectives?

My second question concerns the Apotome feature of
Axiovision. It seems to do the same job as 3D
deconvolution, but how does it work? Why choose one or
the other?

Thank you all in advance.

Stephane-without-i

__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

==============================Original Headers==============================
7, 18 -- From nizets2-at-yahoo.com Fri Apr 7 03:54:20 2006
7, 18 -- Received: from web37402.mail.mud.yahoo.com (web37402.mail.mud.yahoo.com [209.191.87.55])
7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k378sKHp011534
7, 18 -- for {microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 03:54:20 -0500
7, 18 -- Received: (qmail 14286 invoked by uid 60001); 7 Apr 2006 08:54:20 -0000
7, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
7, 18 -- s=s1024; d=yahoo.com;
7, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
7, 18 -- b=OMweDxN77PPBKIar7kn7dRibjJrDypQNfuQV1OKdc7dEFVm651G6nJGH/gAhXPMCsfJkczLt1kWeVV636+rSHsiLxKVmUq/yKZTH+K6Amwn9GgQqnMwE/r83yKnGl3XwuhFTCtuPpjWtQU52gcIEsBoZCXUHXweWZgOVPgGLlkI= ;
7, 18 -- Message-ID: {20060407085420.14284.qmail-at-web37402.mail.mud.yahoo.com}
7, 18 -- Received: from [80.122.101.102] by web37402.mail.mud.yahoo.com via HTTP; Fri, 07 Apr 2006 01:54:20 PDT
7, 18 -- Date: Fri, 7 Apr 2006 01:54:20 -0700 (PDT)
7, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com}
7, 18 -- Subject: Zeiss Axiovert 200M: objectives and axiovision questions
7, 18 -- To: microscopy-at-microscopy.com
7, 18 -- MIME-Version: 1.0
7, 18 -- Content-Type: text/plain; charset=iso-8859-1
7, 18 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: Sven.Terclavers-at-med.kuleuven.be
Date: Fri, 7 Apr 2006 04:44:55 -0500
Subject: [Microscopy] Zeiss Axiovert 200M: objectives and axiovision

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Stephane,

Concerning the objectives, you might want to consider buying an EC
Plan-NeoFluar 40x 1,3 oil. A great objective for fluorescence (offers a
clear & intense image) that I even very often prefer instead of our 100x
oil.

The ApoTome is based on the grid projection theory (more details can be
found on the Zeiss webpage). The difference with Deconvolution software is
that this device (hardware) shows you immediately, while acquiring the
image, the result. The deconvolution software will only show you the result
after acquisition and running the program. If than your image does not turn
out nice, you'll have to restart from the acquisition on, whereas with the
ApoTome, you can immediately see the result and restart if necessary.

Hope it helps a bit!

Best,

Sven Terclavers

-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: vrijdag 7 april 2006 10:58
To: sven.terclavers-at-med.kuleuven.be

Dear listers,

I am currently using our Zeiss axiovert 200M to
observe cell comparments (mitochondria, golgi,
endosomes,...) by fluorescence.
For this purpose, the main "usable" objectives
available are:
- 40x/0,50 LD A-Plan
- 100x/1,3 Oil EC Plan Neofluar

The 40x LD is useful for our life cell imaging
experiments, so I don't think we should change it.
But I wonder if the 100x is really the best solution.
I must say that I am a little bit disappointed by the
quality of my images (I also try to improve the
preparation of the samples!). When i look to the
"images samples" on the CD which was included with the
axiovision soft, I notice that most of the pictures of
cells are taken with a 63x apochromat.
I searched the site of Zeiss and found an interesting
63x apochromat with a NA of 1.4 and corrected for
coverglass thickness (0,17).
What is your experience with the different Zeiss
objectives?

My second question concerns the Apotome feature of
Axiovision. It seems to do the same job as 3D
deconvolution, but how does it work? Why choose one or
the other?

Thank you all in advance.

Stephane-without-i

__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

==============================Original Headers==============================
7, 18 -- From nizets2-at-yahoo.com Fri Apr 7 03:54:20 2006
7, 18 -- Received: from web37402.mail.mud.yahoo.com
(web37402.mail.mud.yahoo.com [209.191.87.55])
7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
k378sKHp011534
7, 18 -- for {microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 03:54:20
-0500
7, 18 -- Received: (qmail 14286 invoked by uid 60001); 7 Apr 2006 08:54:20
-0000
7, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
7, 18 -- s=s1024; d=yahoo.com;
7, 18 --
h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content
-Transfer-Encoding;
7, 18 --
b=OMweDxN77PPBKIar7kn7dRibjJrDypQNfuQV1OKdc7dEFVm651G6nJGH/gAhXPMCsfJkczLt1k
WeVV636+rSHsiLxKVmUq/yKZTH+K6Amwn9GgQqnMwE/r83yKnGl3XwuhFTCtuPpjWtQU52gcIEsB
oZCXUHXweWZgOVPgGLlkI= ;
7, 18 -- Message-ID:
{20060407085420.14284.qmail-at-web37402.mail.mud.yahoo.com}
7, 18 -- Received: from [80.122.101.102] by web37402.mail.mud.yahoo.com via
HTTP; Fri, 07 Apr 2006 01:54:20 PDT
7, 18 -- Date: Fri, 7 Apr 2006 01:54:20 -0700 (PDT)
7, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com}
7, 18 -- Subject: Zeiss Axiovert 200M: objectives and axiovision questions
7, 18 -- To: microscopy-at-microscopy.com
7, 18 -- MIME-Version: 1.0
7, 18 -- Content-Type: text/plain; charset=iso-8859-1
7, 18 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================


Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm


==============================Original Headers==============================
21, 32 -- From Sven.Terclavers-at-med.kuleuven.be Fri Apr 7 04:44:54 2006
21, 32 -- Received: from nibbel.kulnet.kuleuven.ac.be (nibbel.kulnet.kuleuven.ac.be [134.58.240.41])
21, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k379irfc022136
21, 32 -- for {microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 04:44:54 -0500
21, 32 -- Received: from localhost (localhost [127.0.0.1])
21, 32 -- by nibbel.kulnet.kuleuven.ac.be (Postfix) with ESMTP id C841B4CFFF
21, 32 -- for {microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 11:44:52 +0200 (CEST)
21, 32 -- Received: from smtp02.kuleuven.be (lepidus.kulnet.kuleuven.ac.be [134.58.240.72])
21, 32 -- by nibbel.kulnet.kuleuven.ac.be (Postfix) with ESMTP id 235C14CF38
21, 32 -- for {microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 11:44:52 +0200 (CEST)
21, 32 -- Received: from smtp02.kuleuven.be (localhost.localdomain [127.0.0.1])
21, 32 -- by smtp02.kuleuven.be (Postfix) with ESMTP id E1E3E2CAAF8;
21, 32 -- Fri, 7 Apr 2006 11:44:51 +0200 (CEST)
21, 32 -- Received: from gwydion (unknown [134.58.245.176])
21, 32 -- by smtp02.kuleuven.be (Postfix) with ESMTP id BC7842CAAF7
21, 32 -- for {microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 11:44:51 +0200 (CEST)
21, 32 -- Reply-To: {Sven.Terclavers-at-med.kuleuven.be}
21, 32 -- From: "Sven Terclavers" {Sven.Terclavers-at-med.kuleuven.be}
21, 32 -- To: {microscopy-at-microscopy.com}
21, 32 -- Subject: RE: [Microscopy] Zeiss Axiovert 200M: objectives and axiovision questions
21, 32 -- Date: Fri, 7 Apr 2006 11:44:49 +0200
21, 32 -- Organization: VIB CTG-CMVB
21, 32 -- MIME-Version: 1.0
21, 32 -- Content-Type: text/plain;
21, 32 -- charset="us-ascii"
21, 32 -- Content-Transfer-Encoding: 7bit
21, 32 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510
21, 32 -- In-Reply-To: {200604070858.k378wBvh017438-at-ns.microscopy.com}
21, 32 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
21, 32 -- Thread-Index: AcZaIZP+SpOgffk7S42SyyUzGevulQAAMvYg
21, 32 -- Message-Id: {20060407094451.BC7842CAAF7-at-smtp02.kuleuven.be}
21, 32 -- X-Virus-Scanned: by KULeuven Antivirus Cluster
==============================End of - Headers==============================




From: mq7x-at-udcf.gla.ac.uk
Date: Fri, 7 Apr 2006 05:26:36 -0500
Subject: [Microscopy] Substitute for poly-L-lysine in TEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
Interested if anyone knows of a substitute for poly-L-lysine in TEM.
I am currently using it to coat carbon coated formvar coated Ni
grids and examine plasma membranes with immunolabelling.
Fingers crossed.
Martyn.

Martyn Quinn
Henry Wellcome Laboratory for Cell Biology
Division of Biochemistry & Molecular Biology
Davidson Building
Faculty of Biomedical and Life Sciences
University of Glasgow
G12 8QQ

==============================Original Headers==============================
1, 21 -- From mq7x-at-udcf.gla.ac.uk Fri Apr 7 05:26:35 2006
1, 21 -- Received: from hillhead.cent.gla.ac.uk (hillhead.cent.gla.ac.uk [130.209.16.101])
1, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k37AQZLs032136
1, 21 -- for {microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 05:26:35 -0500
1, 21 -- Received: from lenzie.cent.gla.ac.uk ([130.209.16.18])
1, 21 -- by hillhead.cent.gla.ac.uk with esmtp (Exim 4.10)
1, 21 -- id 1FRoAk-0000tA-00
1, 21 -- for microscopy-at-microscopy.com; Fri, 07 Apr 2006 11:26:34 +0100
1, 21 -- Received: from salt.ibls.gla.ac.uk ([130.209.54.27] helo=gould1)
1, 21 -- by lenzie.cent.gla.ac.uk with smtp (Exim 4.50)
1, 21 -- id 1FRoAk-00020g-BR
1, 21 -- for microscopy-at-microscopy.com; Fri, 07 Apr 2006 11:26:34 +0100
1, 21 -- Message-Id: {3.0.1.32.20060407112633.0089de10-at-udcf.gla.ac.uk}
1, 21 -- X-Sender: mq7x-at-udcf.gla.ac.uk
1, 21 -- X-Mailer: Windows Eudora Light Version 3.0.1 (32)
1, 21 -- Date: Fri, 07 Apr 2006 11:26:33 +0100
1, 21 -- To: microscopy-at-microscopy.com
1, 21 -- From: Martyn Quinn {mq7x-at-udcf.gla.ac.uk}
1, 21 -- Subject: Substitute for poly-L-lysine in TEM?
1, 21 -- Mime-Version: 1.0
1, 21 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: hubner-at-iod.krakow.pl
Date: Fri, 7 Apr 2006 05:47:31 -0500
Subject: [Microscopy] FOCOMP'06

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The 5-th International Conference Simulation, Designing and Control of
Foundry Processes in Kraków Poland

http://home.agh.edu.pl/~focomp/

best regards

chris hübner




==============================Original Headers==============================
7, 20 -- From hubner-at-iod.krakow.pl Fri Apr 7 05:47:31 2006
7, 20 -- Received: from mailhost.IOd.krakow.pl (sowa.IOd.krakow.pl [149.156.29.201])
7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k37AlPxX009516
7, 20 -- for {microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 05:47:26 -0500
7, 20 -- Received: from pc2 (pc2 [149.156.29.5])
7, 20 -- by mailhost.IOd.krakow.pl (8.12.10+Sun/8.12.10) with SMTP id k37AdrE7012545
7, 20 -- for {microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 12:40:02 +0200 (CEST)
7, 20 -- Message-ID: {003401c65a2d$aa5baa00$051d9c95-at-iod.krakow.pl}
7, 20 -- From: =?iso-8859-2?Q?KJ_H=FCbner?= {hubner-at-iod.krakow.pl}
7, 20 -- To: {microscopy-at-microscopy.com}
7, 20 -- Subject: FOCOMP'06
7, 20 -- Date: Fri, 7 Apr 2006 12:26:00 +0200
7, 20 -- MIME-Version: 1.0
7, 20 -- Content-Type: text/plain;
7, 20 -- charset="iso-8859-2"
7, 20 -- Content-Transfer-Encoding: 8bit
7, 20 -- X-Priority: 3
7, 20 -- X-MSMail-Priority: Normal
7, 20 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1437
7, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1441
==============================End of - Headers==============================




From: twigg-at-estd.nrl.navy.mil
Date: Fri, 7 Apr 2006 07:46:08 -0500
Subject: [Microscopy] viaWWW: Ion Milling of SiC TEM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both twigg-at-estd.nrl.navy.mil as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: twigg-at-estd.nrl.navy.mil
Name: Mark Twigg

Organization: Naval Research Laboratory

Title-Subject: [Filtered] Ion Milling of SiC TEM samples

Question: I have a perennial problem in ion milling 4H-SiC TEM samples: the thin edge of the electron-transparent region often suffers from crater-like variations in thickness. These divots seem to serve as the focus for bend contours and sample bending that make diffraction contrast analysis and HRTEM imaging difficult at best. Does anybody have any ion milling prescriptions to eliminate this problem?

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Fri Apr 7 07:46:08 2006
6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k37Ck5pJ020680
6, 12 -- for {microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 07:46:07 -0500
6, 12 -- Mime-Version: 1.0
6, 12 -- X-Sender: (Unverified)
6, 12 -- Message-Id: {p06110401c05c0f684418-at-[206.69.208.22]}
6, 12 -- Date: Fri, 7 Apr 2006 07:46:03 -0500
6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: twigg-at-estd.nrl.navy.mil (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Ion Milling of SiC TEM samples
6, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Fri, 7 Apr 2006 07:46:14 -0500
Subject: [Microscopy] Re: Zeiss Axiovert 200M: objectives and axiovision

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

HI Stephane,
We have a Zeiss Axiovert 100 and and Axiovert 200M in our Facility.
The 100 is our confocal instrument. We have the 63x/1.4 NA oil/DIC
lens on both microscopes. It performs beautifully.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

==============================Original Headers==============================
1, 24 -- From lcgould-at-med.cornell.edu Fri Apr 7 07:46:13 2006
1, 24 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45])
1, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k37CkDTs020777
1, 24 -- for {microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 07:46:13 -0500
1, 24 -- Received: from mpx2.med.cornell.edu (pc113142-10.med.cornell.edu [140.251.11.119])
1, 24 -- by smtp-gw2.med.cornell.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id k37CkCjv012304
1, 24 -- for {microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 08:46:12 -0400 (EDT)
1, 24 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23])
1, 24 -- by mpx2.med.cornell.edu
1, 24 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005))
1, 24 -- with ESMTPA id {0IXC007B7RGZ6Q20-at-mpx2.med.cornell.edu} for
1, 24 -- microscopy-at-microscopy.com; Fri, 07 Apr 2006 08:46:12 -0400 (EDT)
1, 24 -- Date: Fri, 07 Apr 2006 08:41:08 -0400
1, 24 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
1, 24 -- Subject: Re: [Microscopy] Zeiss Axiovert 200M: objectives and axiovision
1, 24 -- questions
1, 24 -- In-reply-to: {200604070855.k378tLoB012849-at-ns.microscopy.com}
1, 24 -- Sender: lcgould-at-med.cornell.edu
1, 24 -- To: nizets2-at-yahoo.com, Microscopy Listserver {microscopy-at-microscopy.com}
1, 24 -- Message-id: {p06230901c05c0daee468-at-[140.251.48.23]}
1, 24 -- MIME-version: 1.0
1, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed
1, 24 -- References: {200604070855.k378tLoB012849-at-ns.microscopy.com}
1, 24 -- X-PMX-Version: 5.1.2.240295, Antispam-Engine: 2.3.0.1, Antispam-Data: 2006.4.7.53106
==============================End of - Headers==============================




From: paul_hazelton-at-umanitoba.ca
Date: Fri, 7 Apr 2006 09:05:48 -0500
Subject: [Microscopy] Re: Substitute for poly-L-lysine in TEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Martyn

If what you want is to get rid of hydrophobicity, then Alcian Blue
works, float the grid for a couple of minutes on a 0.1% aqueous
solution, rinse briefly and dry. Other surfactant style wetting agents
are Bacitracin and BSA. Make your solution to 25 microgram/ml with
Bacitracin and mount your grids. Sorry, I don't remember the
concentration of BSA, but it would be about the same.

Alternatively, hydrophobicity 'fades', and if you wait several weeks
the grids are essentially the same as routing formvar.

If the issue is getting the material onto the grids you can try several
methods. First would be direct centrifugation to the grid. Use a
Beckman Airfuge with the EM 90 Rotor. Yeah, like no one saw that
reference coming, right. No, I have no commercial interest in Beckman,
but was involved in some of the work demonstrating utility of the
rotor. If you don't have an Airfuge available, try agar diffusion.
Both methods will concentrate the membranes down onto the grid, but
given the nature of the samples you are using, I suspect Agar diffusion
would work better, especially coupled with Bacitracin. References are
Hammond et al, 1981, J. Clin. Micro., 14:210-221, for the Airfuge, and
Anderson and Doane, 1972, Appl. Microbiol., 24:495-496.

If you want, I do have the concentration protocols in electronic file
form and can share them with you. Sorry, the others are not. Feel free
to call if you want to discuss the procedures.

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Work Phone: 204-789-3313
Pager: 204-931-954
Home Phone: 204-489-6924
Cell: 204-781-1502
Fax: 204-789-3926/204-489-6924







==============================Original Headers==============================
13, 20 -- From paul_hazelton-at-umanitoba.ca Fri Apr 7 09:05:48 2006
13, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23])
13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k37E5lvX008389
13, 20 -- for {microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 09:05:48 -0500
13, 20 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160])
13, 20 -- (authenticated bits=0)
13, 20 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id k37E5kgk012615;
13, 20 -- Fri, 7 Apr 2006 09:05:46 -0500 (CDT)
13, 20 -- Message-ID: {443671B6.5050109-at-umanitoba.ca}
13, 20 -- Date: Fri, 07 Apr 2006 09:05:42 -0500
13, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca}
13, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax)
13, 20 -- X-Accept-Language: en-us, en
13, 20 -- MIME-Version: 1.0
13, 20 -- To: mq7x-at-udcf.gla.ac.uk
13, 20 -- Subject: Re: [Microscopy] Substitute for poly-L-lysine in TEM?
13, 20 -- References: {200604071028.k37ASWrL003471-at-ns.microscopy.com}
13, 20 -- In-Reply-To: {200604071028.k37ASWrL003471-at-ns.microscopy.com}
13, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
13, 20 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: SBagley-at-PICR.man.ac.uk
Date: Fri, 7 Apr 2006 09:55:44 -0500
Subject: [Microscopy] Zeiss Axiovert 200M: objectives and axiovision

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Hi Stephane

I utilise the Alpha Plan-Fluar x100 1.45 NA objective lens on my
Axiovert 200M for live cell imaging of microtubual dynamics in S.pombe,
nuclear pore assembly and mitochondrial dynamics in single mammalian
cells, over time periods of an hour to three days. I must admit to
worrying about maintenance of the lens-oil-coverslip interface over such
a time frame but the oil becoming too runny or drying up over this time
frame does not seem to be a problem. Originally all of our work was
carried out using the x63 apochromat but the x100 1.45 NA lens is much
more light efficient.

I agree with Sven, the EC Plan-NeoFluar 40x 1.3 oil is a great lens, we
utilise this for cell invasion assays and can image samples for three to
five days. As well as heating the sample (Bioptechs), CO2/Air and
environmental chamber (Solent), we also heat the objective lens with the
Bioptechs objective heater.

We were so impressed with the alpha-plan lens we purchased another for
the Olympus based Deltavision system,

All the best

Steve





Steve Bagley
Associate Scientist
Advanced Imaging Facility
Paterson Institute for Cancer Research
Cancer Research UK
University of Manchester
Wilmslow Road
Manchester
M20 4BX, UK


http://www.paterson.man.ac.uk/facilities/advimg.jsp




-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: 07 April 2006 09:59
To: Steve Bagley

Dear listers,

I am currently using our Zeiss axiovert 200M to observe cell comparments
(mitochondria, golgi,
endosomes,...) by fluorescence.
For this purpose, the main "usable" objectives available are:
- 40x/0,50 LD A-Plan
- 100x/1,3 Oil EC Plan Neofluar

The 40x LD is useful for our life cell imaging experiments, so I don't
think we should change it.
But I wonder if the 100x is really the best solution.
I must say that I am a little bit disappointed by the quality of my
images (I also try to improve the preparation of the samples!). When i
look to the "images samples" on the CD which was included with the
axiovision soft, I notice that most of the pictures of cells are taken
with a 63x apochromat.
I searched the site of Zeiss and found an interesting 63x apochromat
with a NA of 1.4 and corrected for coverglass thickness (0,17).
What is your experience with the different Zeiss objectives?

My second question concerns the Apotome feature of Axiovision. It seems
to do the same job as 3D deconvolution, but how does it work? Why choose
one or the other?

Thank you all in advance.

Stephane-without-i

__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

==============================Original
Headers==============================
7, 18 -- From nizets2-at-yahoo.com Fri Apr 7 03:54:20 2006 7, 18 --
Received: from web37402.mail.mud.yahoo.com (web37402.mail.mud.yahoo.com
[209.191.87.55])
7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
id k378sKHp011534
7, 18 -- for {microscopy-at-microscopy.com} ; Fri, 7 Apr 2006
03:54:20 -0500
7, 18 -- Received: (qmail 14286 invoked by uid 60001); 7 Apr 2006
08:54:20 -0000 7, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
7, 18 -- s=s1024; d=yahoo.com;
7, 18 --
h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Con
tent-Transfer-Encoding;
7, 18 --
b=OMweDxN77PPBKIar7kn7dRibjJrDypQNfuQV1OKdc7dEFVm651G6nJGH/gAhXPMCsfJkcz
Lt1kWeVV636+rSHsiLxKVmUq/yKZTH+K6Amwn9GgQqnMwE/r83yKnGl3XwuhFTCtuPpjWtQU
52gcIEsBoZCXUHXweWZgOVPgGLlkI= ;
7, 18 -- Message-ID:
{20060407085420.14284.qmail-at-web37402.mail.mud.yahoo.com}
7, 18 -- Received: from [80.122.101.102] by web37402.mail.mud.yahoo.com
via HTTP; Fri, 07 Apr 2006 01:54:20 PDT 7, 18 -- Date: Fri, 7 Apr 2006
01:54:20 -0700 (PDT) 7, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com}
7, 18 -- Subject: Zeiss Axiovert 200M: objectives and axiovision
questions 7, 18 -- To: microscopy-at-microscopy.com 7, 18 -- MIME-Version:
1.0 7, 18 -- Content-Type: text/plain; charset=iso-8859-1 7, 18 --
Content-Transfer-Encoding: 8bit ==============================End of -
Headers==============================

--------------------------------------------------------


This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.



==============================Original Headers==============================
20, 28 -- From SBagley-at-PICR.man.ac.uk Fri Apr 7 09:55:44 2006
20, 28 -- Received: from probity.mcc.ac.uk (probity.mcc.ac.uk [130.88.200.94])
20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k37Eth0j018425
20, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 09:55:43 -0500
20, 28 -- Received: from jill.picr.man.ac.uk ([130.88.233.248] helo=sanmail.picr.man.ac.uk)
20, 28 -- by probity.mcc.ac.uk with esmtp (Exim 4.60 (FreeBSD))
20, 28 -- (envelope-from {SBagley-at-PICR.man.ac.uk} )
20, 28 -- id 1FRsND-0003su-3L
20, 28 -- for Microscopy-at-microscopy.com; Fri, 07 Apr 2006 15:55:43 +0100
20, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.1830
20, 28 -- Content-class: urn:content-classes:message
20, 28 -- MIME-Version: 1.0
20, 28 -- Content-Type: text/plain;
20, 28 -- charset="us-ascii"
20, 28 -- Importance: normal
20, 28 -- Priority: normal
20, 28 -- Subject: RE: [Microscopy] Zeiss Axiovert 200M: objectives and axiovision questions
20, 28 -- Date: Fri, 7 Apr 2006 15:55:42 +0100
20, 28 -- Message-ID: {BAA35444B19AD940997ED02A6996AAE002A7BE78-at-sanmail.picr.man.ac.uk}
20, 28 -- X-MS-Has-Attach:
20, 28 -- X-MS-TNEF-Correlator:
20, 28 -- Thread-Topic: [Microscopy] Zeiss Axiovert 200M: objectives and axiovision questions
20, 28 -- Thread-Index: AcZaIaYcLhOYRwlgSCGxIooq6mB5IwAChkrA
20, 28 -- From: "Steve Bagley" {SBagley-at-PICR.man.ac.uk}
20, 28 -- To: {Microscopy-at-microscopy.com}
20, 28 -- X-UoM: Scanned by the University Mail System. See http://www.itservices.manchester.ac.uk/email/filtering/information/ for details.
20, 28 -- Content-Transfer-Encoding: 8bit
20, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k37Eth0j018425
==============================End of - Headers==============================




From: amich-at-ufl.edu
Date: Fri, 7 Apr 2006 12:38:17 -0500
Subject: [Microscopy] bacterial adhesion to the substrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,
I need to demonstrate bacteria on the antibacterial cellulose
substrate; the antibacterial agent is assumingly kills bacteria on
the contact, and my goal is to demonstrate collapsed membrane. I
have problem with the E.coli and S.aureus attachment. Any
suggestions on the improving the adhesion would be highly
appreciated.
Thank you, Albina
PS thank you all for the helpful advice on vapor fixation!


--
MIKHAYLOVA,ALBINA, PhD
Post Doctoral Research Associate
Materials Science and Engineering
University of Florida


==============================Original Headers==============================
4, 21 -- From amich-at-ufl.edu Fri Apr 7 12:38:17 2006
4, 21 -- Received: from smtp.ufl.edu (smtp02.osg.ufl.edu [128.227.74.165])
4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k37HcHcV031129
4, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 12:38:17 -0500
4, 21 -- Received: from osgjas02.cns.ufl.edu (osgjas02.cns.ufl.edu [128.227.74.132])
4, 21 -- by smtp.ufl.edu (8.13.6/8.13.6/2.5.1) with ESMTP id k37HcD4i3170342
4, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Apr 2006 13:38:13 -0400
4, 21 -- Message-ID: {73562524.302501144431493039.JavaMail.osg-at-osgjas02.cns.ufl.edu}
4, 21 -- Date: Fri, 7 Apr 2006 13:38:13 -0400 (EDT)
4, 21 -- From: "MIKHAYLOVA,ALBINA" {amich-at-ufl.edu}
4, 21 -- To: Microscopy-at-microscopy.com
4, 21 -- Subject: bacterial adhesion to the substrate
4, 21 -- MIME-Version: 1.0
4, 21 -- Content-Type: text/plain; format=flowed; charset=us-ascii
4, 21 -- Content-Transfer-Encoding: 7bit
4, 21 -- X-Mailer: GatorMail WebMail (http://GatorMail.sf.net/)
4, 21 -- X-Originating-IP: 72.155.88.211 [72.155.88.211]
4, 21 -- X-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED
4, 21 -- X-UFL-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED
4, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/)
4, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/)
==============================End of - Headers==============================




From: bliss5-at-llnl.gov
Date: Sat, 8 Apr 2006 10:54:03 -0500
Subject: [Microscopy] viaWWW: Lacy grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both bliss5-at-llnl.gov as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: bliss5-at-llnl.gov
Name: R. Ann Bliss

Organization: Lawrence Livermore National Lab

Title-Subject: [Filtered] Lacy grids

Question: I was about to give a colleague ordering information for lacey grids when another mentioned finding silicone contamination on these grids in the past. Has anyone found this to be true?
Is there one brand better/worse than the others?

You could reply off-list if this is not an appropriate question for publication.

---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Sat Apr 8 10:54:03 2006
7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k38Fs1tk001565
7, 12 -- for {microscopy-at-microscopy.com} ; Sat, 8 Apr 2006 10:54:02 -0500
7, 12 -- Mime-Version: 1.0
7, 12 -- X-Sender: (Unverified)
7, 12 -- Message-Id: {p06110408c05d8d01b598-at-[206.69.208.22]}
7, 12 -- Date: Sat, 8 Apr 2006 10:54:01 -0500
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: bliss5-at-llnl.gov (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: Lacy grids
7, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: jerzy.gazda-at-ceriumlabs.com
Date: Sat, 8 Apr 2006 10:54:38 -0500
Subject: [Microscopy] viaWWW: CCD cameras for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jerzy.gazda-at-ceriumlabs.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: jerzy.gazda-at-ceriumlabs.com
Name: Jerzy Gazda

Organization: CeriumLabs

Title-Subject: [Filtered] CCD cameras for TEM

Question: Hi,
I am looking for suggestions (including from vendors - off list) for a digital camera for TEM imaging on JEM2010 used primary for metrology (2kx2K maximum, imaging speed is of essence). The camera needs to be installed below viewing chamber on the instrument and come with modern acquizition software. I am aware of three major competitors in that market, but might there be others?

User suggestiosn are specially welcome.

Thanks,

Jerzy

---------------------------------------------------------------------------

==============================Original Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Sat Apr 8 10:54:38 2006
9, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k38FsbBa001961
9, 12 -- for {microscopy-at-microscopy.com} ; Sat, 8 Apr 2006 10:54:37 -0500
9, 12 -- Mime-Version: 1.0
9, 12 -- X-Sender: (Unverified)
9, 12 -- Message-Id: {p06110409c05d8d20bd01-at-[206.69.208.22]}
9, 12 -- Date: Sat, 8 Apr 2006 10:54:36 -0500
9, 12 -- To: microscopy-at-microscopy.com
9, 12 -- From: jerzy.gazda-at-ceriumlabs.com (by way of MicroscopyListserver)
9, 12 -- Subject: viaWWW: CCD cameras for TEM
9, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: cgarber-at-2spi.com
Date: Sat, 8 Apr 2006 15:08:04 -0500
Subject: [Microscopy] Silicone contamination on lacey carbon filmed grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

R. Ann Bliss asked the following:
==================================================================
Question: I was about to give a colleague ordering information for lacey
grids when another mentioned finding silicone contamination on these grids
in the past. Has anyone found this to be true?
Is there one brand better/worse than the others?

You could reply off-list if this is not an appropriate question for
publication.
==================================================================
In my opinion, it is always an "appropriate" question for discussion if a
quality issue could have an impact, especially if undesirable, on the
quality of one's results.

It has been our experience that there are two main sources for potential Si
contamination on either carbon or holey carbon or lacey carbon coated grids:

a) the vacuum evaporator itself, if it has been used for the evaporation of
SiOx. It is extremely difficult to remove the last vestiges of the
evaporated SiOx from previous evaporations. Another, but not so common
source is the case where someone thinks they should be using silicone fluid
in a DP of a vacuum evaporator with the obvious potential for contamination.
It has not been uncommon for me to find that silicone grease instead of a
pure hydrocarbon grease (e.g. Apiezon M or L or Santovac 5GB high vacuum
grease) is sometimes being used on the bell jar o ring.

b) TEM grid storage boxes which apparently in some instances have been
molded by molders who, in an effort to speed up the molding, and reduce
costs, use a silicone release agent.

Another source for Si can be from the carbon rods if one is not using the
very highest spectrographic purity (which do of course cost more than rods
with lesser purity). As a further comment, most laboratories with vacuum
evaporators have only one, and with the passing of time, it is hard to know
exactly what has and has not been evaporated in them over the years. If the
system is turbo pumped, there would not be any chance of silicone fluid
contamination but when it comes to the carbon rods, we know that not all
laboratories are using rods of the highest purity for their evaporations. I
don't want to sound like I am promoting the use of turbo pumped systems
since we are hard pressed to show that there is any real difference in the
quality of the carbon films produced, turbo vs. DP systems.

SPI Supplies is a major manufacturer of custom coated grids for EM. All
carbon evaporations are done in high vacuum systems that have never been
used for SiOx evaporation and have never been used with silicone fluids or
greases. And grid storage boxes sold by SPI Supplies or BEEM, to our
knowledge, have never been molded with the aid of any silicone release
agents. The carbon source is carbon rods of spectrographic purity. We
have had, from time to time, and I mean perhaps once a year, a customer tell
us that they are detecting Si on their coated grids. But we have never been
able to reproduce, in our own TEM/EDS instrumentation, evidence for that
contamination ourselves using retained samples from the same batch. There
have been times when we have duplicated the observation of Si on coated
grids that were returned to us, but not from the retained samples, causing
us to conclude that the contamination occurred after the grids left SPI
Supplies.

We are as baffled by this as anyone else and any additional clues as to
where such Si contamination comes from would be helpful. So far as we know,
Si contamination has not been a problem associated with the coated grids
from SPI Supplies.

Disclaimer: SPI Supplies is a long time manufacturer of custom coated grids
and further information can be found on URL
http://www.2spi.com/catalog/grids/cusctgrd.html

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



==============================Original Headers==============================
15, 27 -- From cgarber-at-2spi.com Sat Apr 8 15:08:03 2006
15, 27 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43])
15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k38K83kE023640
15, 27 -- for {microscopy-at-msa.microscopy.com} ; Sat, 8 Apr 2006 15:08:03 -0500
15, 27 -- Received: from yourb27fb1c401 ([71.225.86.11])
15, 27 -- (authenticated bits=0)
15, 27 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id k38K7vWp024262;
15, 27 -- Sat, 8 Apr 2006 16:08:01 -0400
15, 27 -- X-IDV-FirstRcvd: [71.225.86.11]
15, 27 -- X-IDV-HELO: yourb27fb1c401
15, 27 -- X-IDV-Authenticated-User: cgarber
15, 27 -- Message-ID: {01d201c65b48$21a4f6f0$6501a8c0-at-yourb27fb1c401}
15, 27 -- From: "Garber, Charles A" {cgarber-at-2spi.com}
15, 27 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com}
15, 27 -- Cc: {bliss5-at-llnl.gov}
15, 27 -- Subject: Silicone contamination on lacey carbon filmed grids
15, 27 -- Date: Sat, 8 Apr 2006 16:07:57 -0400
15, 27 -- MIME-Version: 1.0
15, 27 -- Content-Type: text/plain;
15, 27 -- format=flowed;
15, 27 -- charset="iso-8859-1";
15, 27 -- reply-type=original
15, 27 -- Content-Transfer-Encoding: 7bit
15, 27 -- X-Priority: 3
15, 27 -- X-MSMail-Priority: Normal
15, 27 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180
15, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
==============================End of - Headers==============================




From: w_d_howell-at-yahoo.com
Date: Sat, 8 Apr 2006 19:38:35 -0500
Subject: [Microscopy] viaWWW: Comparison of Zeiss objectives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both w_d_howell-at-yahoo.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: w_d_howell-at-yahoo.com
Name: Bill Howell

Organization: Stanford

Title-Subject: [Filtered] Comparison of Zeiss objectives

Question: Hello all,

Is there any practical advantage to the Zeiss Plan-Apochromat 5X/0.16 M27 (420630-9900-000) relative to the Zeiss EC Plan-Neofluar 5X/0.15 M27 (420330-9900-000)? My intended application is general histology/pathology examining primarily H&E and immunohistochemistry slides, but also taking some low magnification digital photos. M27 refers to the screw thread on the objective.

Also, what are the relative merits of the Zeiss Plan-Apochromat 40X/0.95 Corr (dry) M27 (420660-9970-000) compared to Zeiss C-Plan-Apochromat 40X/1.20 Corr UV-VIS-IR (water) M27 (421767-9970-000)? The intended applications will be the same as above, but will definitely be used for digital photos and DIC. There is some possiblity that the objectives may be used for confocal or Apotome (structured illumination) work at some point in the future.

Thanks in advance for your responses.

Regards,

Bill Howell

---------------------------------------------------------------------------

==============================Original Headers==============================
11, 12 -- From zaluzec-at-microscopy.com Sat Apr 8 19:38:35 2006
11, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k390cZSv013833
11, 12 -- for {microscopy-at-microscopy.com} ; Sat, 8 Apr 2006 19:38:35 -0500
11, 12 -- Mime-Version: 1.0
11, 12 -- X-Sender: (Unverified)
11, 12 -- Message-Id: {p06110415c05e07f88826-at-[206.69.208.22]}
11, 12 -- Date: Sat, 8 Apr 2006 19:38:34 -0500
11, 12 -- To: microscopy-at-microscopy.com
11, 12 -- From: w_d_howell-at-yahoo.com (by way of MicroscopyListserver)
11, 12 -- Subject: viaWWW: Comparison of Zeiss objectives
11, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: zaluzec-at-microscopy.com
Date: Sun, 9 Apr 2006 09:38:11 -0500
Subject: [Microscopy] Si Contamination on Lacy grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

How was this Si contamination detected and/or characterized?

How were the grids stored. It is important to distinguish
possible manufacturing issues from inappropriate handling.

Nestor
Your Friendly Neighborhood SysOp



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
7, 13 -- From zaluzec-at-microscopy.com Sun Apr 9 09:38:10 2006
7, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k39EcAaJ010045
7, 13 -- for {microscopy-at-microscopy.com} ; Sun, 9 Apr 2006 09:38:10 -0500
7, 13 -- Mime-Version: 1.0
7, 13 -- Message-Id: {p06110417c05ecb795b4e-at-[206.69.208.22]}
7, 13 -- In-Reply-To: {200604081554.k38Fs3Mn001579-at-ns.microscopy.com}
7, 13 -- References: {200604081554.k38Fs3Mn001579-at-ns.microscopy.com}
7, 13 -- Date: Sun, 9 Apr 2006 09:38:09 -0500
7, 13 -- To: microscopy-at-microscopy.com
7, 13 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
7, 13 -- Subject: Si Contamination on Lacy grids
7, 13 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: dkoleary-at-verizon.net
Date: Sun, 9 Apr 2006 15:01:26 -0500
Subject: [Microscopy] LM, Polarized Light Microscopy Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

New York Microscopical Society
30 North Mountain Avenue
Montclair, NJ 07042

Bernard Friedman Memorial Workshop

Polarized Light Microscopy
April 29, May 6, 13 & 20, 2006

An advanced course on polarized light microscopy which will cover the
following topics:
The nature of polarized light
The origin and interpretation of interference colors
Birefringence and crystal orientation, The Indicatrix
Compensation and variable compensators
Interference figures and their interpretation

The workshop will consist of four Consecutive Saturdays of lectures and
hands on labs to cover the theoretical and practical aspects of polarized
light microscopy. The course instructors include Jan Hinsch of Leica, Inc.,
Mary McCann of McCann Imaging, John Reffner of SensIR and N.Y.M.S.
Instructor Don O'Leary.

WHEN: April 2, May 6, 13 & 20, 2006 from 10 A.M. to 4 P.M.

WHERE: 30 North Mountain Avenue, Montclair, NJ 07042. Phone (973) 744-0043

COST: $395 for N.Y.M.S. members, $425 for non-members (includes membership).
Lunch and course materials are included. Checks made out to N.Y.M.S.

WHO: advanced course for those who have completed "The Use of the
Microscope" or are experienced in microscopy and familiar with the theory of
its use.

HOW: Register using the form below. Limited to the first 12 registrants.
Return form to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.

FURTHER INFORMATION: Call D. O'Leary (201)797-8849 e-mail
dkoleary-at-verizon.net

PLEASE POST
--------------------------------------------------------------------------
Registration Form
Polarized Light Microscopy

N.Y.M.S. Member_________________ ($395) Non-Member__________($425)

Name_____________________________________________________________
Address___________________________________________________________
Phone (W)_________________________(H)______________________________
e-mail________________________________________




==============================Original Headers==============================
17, 19 -- From dkoleary-at-verizon.net Sun Apr 9 15:01:26 2006
17, 19 -- Received: from smtp101.vzn.mail.dcn.yahoo.com (smtp101.vzn.mail.dcn.yahoo.com [209.73.179.139])
17, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k39K1QN0023634
17, 19 -- for {Microscopy-at-Microscopy.Com} ; Sun, 9 Apr 2006 15:01:26 -0500
17, 19 -- Received: (qmail 7557 invoked from network); 9 Apr 2006 20:01:25 -0000
17, 19 -- Received: from unknown (HELO DonsLaptop) (dkoleary-at-verizon.net-at-71.250.121.73 with login)
17, 19 -- by smtp101.vzn.mail.dcn.yahoo.com with SMTP; 9 Apr 2006 20:01:25 -0000
17, 19 -- From: "Donald O'Leary" {dkoleary-at-verizon.net}
17, 19 -- To: "Microscopy List Server" {Microscopy-at-Microscopy.Com}
17, 19 -- Subject: LM, Polarized Light Microscopy Workshop
17, 19 -- Date: Sun, 9 Apr 2006 16:01:17 -0400
17, 19 -- Message-ID: {000001c65c10$634f95b0$0300a8c0-at-DonsLaptop}
17, 19 -- MIME-Version: 1.0
17, 19 -- Content-Type: text/plain;
17, 19 -- charset="us-ascii"
17, 19 -- Content-Transfer-Encoding: 7bit
17, 19 -- X-Mailer: Microsoft Office Outlook 11
17, 19 -- Thread-Index: AcZcEFzu5SQQMO+YRQq9YXnU4pP4cQ==
17, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2670
==============================End of - Headers==============================




From: mq7x-at-udcf.gla.ac.uk
Date: Mon, 10 Apr 2006 05:42:17 -0500
Subject: [Microscopy] Substitute for poly-L in TEM - more info.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
Many thanks for the suggestions and to those asking sensibly 'why?'.
To clarify a bit:
I am glow discharging the grids before applying the poly-L.
The poly-L is being used as an adhesive.
Unfortunately I may be getting some form of artefact as a result of
an interaction between the negative stain and the poly-L (?).
I was hoping to be able to utilize a different 'adhesive' and
pinpoint the problem.
Many thanks,
Martyn.
Martyn Quinn
Henry Wellcome Laboratory for Cell Biology
Division of Biochemistry & Molecular Biology
Davidson Building
Faculty of Biomedical and Life Sciences
University of Glasgow
G12 8QQ

==============================Original Headers==============================
1, 21 -- From mq7x-at-udcf.gla.ac.uk Mon Apr 10 05:42:16 2006
1, 21 -- Received: from hillend.cent.gla.ac.uk (hillend.cent.gla.ac.uk [130.209.16.102])
1, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3AAgGJT011349
1, 21 -- for {microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 05:42:16 -0500
1, 21 -- Received: from lenzie.cent.gla.ac.uk ([130.209.16.18])
1, 21 -- by hillend.cent.gla.ac.uk with esmtp (Exim 4.10)
1, 21 -- id 1FStqZ-0000eR-00
1, 21 -- for microscopy-at-microscopy.com; Mon, 10 Apr 2006 11:42:15 +0100
1, 21 -- Received: from db241-26.ibls.gla.ac.uk ([130.209.54.26] helo=lab204)
1, 21 -- by lenzie.cent.gla.ac.uk with smtp (Exim 4.50)
1, 21 -- id 1FStqX-0003R7-Hk
1, 21 -- for microscopy-at-microscopy.com; Mon, 10 Apr 2006 11:42:15 +0100
1, 21 -- Message-Id: {3.0.5.32.20060410114213.007f7330-at-udcf.gla.ac.uk}
1, 21 -- X-Sender: mq7x-at-udcf.gla.ac.uk
1, 21 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.5 (32)
1, 21 -- Date: Mon, 10 Apr 2006 11:42:13 +0100
1, 21 -- To: microscopy-at-microscopy.com
1, 21 -- From: Martyn Quinn {mq7x-at-udcf.gla.ac.uk}
1, 21 -- Subject: Substitute for poly-L in TEM - more info.
1, 21 -- Mime-Version: 1.0
1, 21 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: c.muncke-at-liverpool.ac.uk
Date: Mon, 10 Apr 2006 07:01:38 -0500
Subject: [Microscopy] Re: Substitute for poly-L in TEM - more info.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Martyn,

Ah, what kind of staining artefact are you getting then? And what
staining method do you use?

I have not yet experienced staining artefacts related to the poly-L-
lysine coating in plasma membrane sheet preparation, but maybe the
problem lies somewhere else in the method?

Regards,

Cornelia Muncke

EM-Unit
Physiological Laboratory
University of Liverpool
Crown Street
Liverpool
L69 3BX



}
} Hello,
} Many thanks for the suggestions and to those asking sensibly
} 'why?'.
} To clarify a bit:
} I am glow discharging the grids before applying the poly-L.
} The poly-L is being used as an adhesive.
} Unfortunately I may be getting some form of artefact as a
} result of
} an interaction between the negative stain and the poly-L (?).
} I was hoping to be able to utilize a different 'adhesive' and
} pinpoint the problem.
} Many thanks,
} Martyn.
} Martyn Quinn
} Henry Wellcome Laboratory for Cell Biology
} Division of Biochemistry & Molecular Biology
} Davidson Building
} Faculty of Biomedical and Life Sciences
} University of Glasgow
} G12 8QQ
}

==============================Original Headers==============================
9, 28 -- From c.muncke-at-liverpool.ac.uk Mon Apr 10 07:01:38 2006
9, 28 -- Received: from mx2.liv.ac.uk (mx2.liv.ac.uk [138.253.100.180])
9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3AC1cNM021883
9, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 10 Apr 2006 07:01:38 -0500
9, 28 -- Received: from mailhuba.liv.ac.uk ([138.253.100.36])
9, 28 -- by mx2.liv.ac.uk with esmtp (Exim 4.54)
9, 28 -- id 1FSv5J-0000ts-BO
9, 28 -- for Microscopy-at-Microscopy.Com; Mon, 10 Apr 2006 13:01:33 +0100
9, 28 -- Received: from localhost ([127.0.0.1] helo=mailhuba.liv.ac.uk)
9, 28 -- by mailhuba.liv.ac.uk with esmtp (Exim 4.54)
9, 28 -- id 1FSv5J-0000B5-AV
9, 28 -- for Microscopy-at-Microscopy.Com; Mon, 10 Apr 2006 13:01:33 +0100
9, 28 -- Received: from pc028200.med.liv.ac.uk ([138.253.28.200])
9, 28 -- by mailhuba.liv.ac.uk with esmtpsa (TLSv1:RC4-SHA:128)
9, 28 -- (Exim 4.54)
9, 28 -- id 1FSv5J-0000Av-9t
9, 28 -- for Microscopy-at-Microscopy.Com; Mon, 10 Apr 2006 13:01:33 +0100
9, 28 -- Mime-Version: 1.0 (Apple Message framework v749.3)
9, 28 -- In-Reply-To: {200604101049.k3AAnZSG020912-at-ns.microscopy.com}
9, 28 -- References: {200604101049.k3AAnZSG020912-at-ns.microscopy.com}
9, 28 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
9, 28 -- Message-Id: {FD2EFD1B-D948-46A2-B0E1-9FFA084BDBB5-at-liverpool.ac.uk}
9, 28 -- Content-Transfer-Encoding: 7bit
9, 28 -- From: Cornelia Muncke {c.muncke-at-liverpool.ac.uk}
9, 28 -- Subject: Re: [Microscopy] Substitute for poly-L in TEM - more info.
9, 28 -- Date: Mon, 10 Apr 2006 12:58:28 +0100
9, 28 -- To: Microscopy-at-Microscopy.Com
9, 28 -- X-Mailer: Apple Mail (2.749.3)
==============================End of - Headers==============================




From: anatolebeams-at-abdm.co.uk
Date: Mon, 10 Apr 2006 08:27:59 -0500
Subject: [Microscopy] viaWWW: Pixera digital cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both anatolebeams-at-abdm.co.uk as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: anatolebeams-at-abdm.co.uk
Name: Anatole Beams

Organization: Natural History Museum, London

Title-Subject: [Filtered] Pixera digital cameras

Question: We have a Pixera PVC100C camera that we haven't used for a while. Now that we come to need it the PhotoShop plug-in has been lost in an upgrade on the Mac it was originally installed on. Pixera won't supply one as they no longer support the camera. Would anyone, by any chance, be able to supply us with just the PhotoShop plug-in, or know where we might find one. I imagine it is more-or-less the same for all their cameras.

Many thanks

Anatole Beams

---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Mon Apr 10 08:27:59 2006
8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3ADRuRA032562
8, 12 -- for {microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 08:27:59 -0500
8, 12 -- Mime-Version: 1.0
8, 12 -- X-Sender: (Unverified)
8, 12 -- Message-Id: {p06110400c0600db5e2d6-at-[206.69.208.22]}
8, 12 -- Date: Mon, 10 Apr 2006 08:27:55 -0500
8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: anatolebeams-at-abdm.co.uk (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: Pixera digital cameras
8, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: raristau-at-ims.uconn.edu
Date: Mon, 10 Apr 2006 09:23:36 -0500
Subject: [Microscopy] re: Silicone contamination on lacey carbon filmed grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I contribute this note only to broaden the discussion a little.

I confess to have accused my grid supplier, some years ago, of substituting
SiOx coated grids for the C-coated grids I ordered. Further investigation of
our lab (not UConn) uncovered a TEM user who routinely stuck her grids to
the milling post with vacuum grease. This Si-based grease was then
transferred to the TEM sample holder.

A thorough cleaning of sample holder and change in sample prep protocol
cleared up this problem.

Cheers

--
Roger A. Ristau, PhD
TEM Specialist
Institute of Materials Science
97 North Eagleville Road
University of Connecticut
Storrs, CT 06269
vox: 860-486-5379
fax: 860-486-4745



==============================Original Headers==============================
7, 19 -- From raristau-at-ims.uconn.edu Mon Apr 10 09:23:36 2006
7, 19 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204])
7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3AENY9g010310
7, 19 -- for {microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 09:23:35 -0500
7, 19 -- Received: from [137.99.20.202] (d20h202.public.uconn.edu [137.99.20.202])
7, 19 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id k3AEN2J2026507
7, 19 -- for {microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 10:23:02 -0400
7, 19 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0
7, 19 -- Date: Mon, 10 Apr 2006 10:22:30 -0400
7, 19 -- Subject: re: Silicone contamination on lacey carbon filmed grids
7, 19 -- From: Roger Ristau {raristau-at-ims.uconn.edu}
7, 19 -- To: {microscopy-at-microscopy.com}
7, 19 -- Message-ID: {C05FE266.B79%raristau-at-ims.uconn.edu}
7, 19 -- Mime-version: 1.0
7, 19 -- Content-type: text/plain; charset="US-ASCII"
7, 19 -- Content-transfer-encoding: 7bit
7, 19 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information.
7, 19 -- X-UConn-MailScanner: Found to be clean
7, 19 -- X-UConn-MailScanner-SpamCheck:
==============================End of - Headers==============================




From: tonygr-at-MIT.EDU
Date: Mon, 10 Apr 2006 10:51:21 -0500
Subject: [Microscopy] NESM Woods Hole meeting, 2006

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The New England Society for Microscopy is pleased to announce its
annual spring meeting, incorporating a workshop and the Spring
Symposium, to be held at the Marine Biological Laboratory at Woods
Hole, MA, May 4-6 2006.

The workshop, held on Thursday 4th. May will, this year, be on the
topic of Confocal Microscopy. The symposium, held on Friday and
Saturday, May 5-6, will feature, amongst other things, a panel and
open discussion, moderated by John Mackenzie, on the topic of "The
ethics of digital imaging and storage". The usual exhibit by the
Society's corporate members will take place on Saturday
morning. There will be a poster session, for which submissions are solicited.

Full details of the meeting are available on the Society's web pages,
at http://nesm.cims.harvard.edu by following the link to "upcoming events".

Please note that advance registration for both events is
essential. The registration deadline for the workshop is Friday
April 21st. Registration for the symposium dinner must be received
by Thursday April 20th. Room reservations at MBL are available on a
first-come basis.

Tony Garratt-Reed - for NESM



***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA

Tel: 617-253-4622
Fax: 617-258-6478
************************************************


==============================Original Headers==============================
10, 25 -- From tonygr-at-MIT.EDU Mon Apr 10 10:51:21 2006
10, 25 -- Received: from biscayne-one-station.mit.edu (BISCAYNE-ONE-STATION.MIT.EDU [18.7.7.80])
10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3AFpKvc021176
10, 25 -- for {microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 10:51:21 -0500
10, 25 -- Received: from outgoing.mit.edu (OUTGOING-AUTH.MIT.EDU [18.7.22.103])
10, 25 -- by biscayne-one-station.mit.edu (8.13.6/8.9.2) with ESMTP id k3AFkIq4014679
10, 25 -- for {microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 11:46:18 -0400 (EDT)
10, 25 -- Received: from emlab.mit.edu (EMLAB.MIT.EDU [18.82.0.121])
10, 25 -- (authenticated bits=0)
10, 25 -- (User authenticated as tonygr-at-ATHENA.MIT.EDU)
10, 25 -- by outgoing.mit.edu (8.13.6/8.12.4) with ESMTP id k3AFkBYb014186
10, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
10, 25 -- for {microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 11:46:12 -0400 (EDT)
10, 25 -- Message-Id: {6.2.3.4.2.20060410113029.02e052b0-at-po9.mit.edu}
10, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4
10, 25 -- Date: Mon, 10 Apr 2006 11:46:09 -0400
10, 25 -- To: microscopy-at-microscopy.com
10, 25 -- From: Anthony Garratt-Reed {tonygr-at-MIT.EDU}
10, 25 -- Subject: NESM Woods Hole meeting, 2006
10, 25 -- Mime-Version: 1.0
10, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
10, 25 -- X-Spam-Score: 1.217
10, 25 -- X-Spam-Level: * (1.217)
10, 25 -- X-Spam-Flag: NO
10, 25 -- X-Scanned-By: MIMEDefang 2.42
==============================End of - Headers==============================




From: michael-at-Shaffer.net
Date: Mon, 10 Apr 2006 11:22:12 -0500
Subject: [Microscopy] Ethics & Digital Imaging (long)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The other day I mistakenly destroyed relavent information regarding a SEM
digital image. I instantly realized what I had done (... what a blunder!),
and I should have known better. The mistake reminded me I had intended to
read the article entitled "Ethics and Digital Imaging" (Microscopy Today,
V14,N1, Jan06).

The article's recommendations surprised me a bit relative to what I had
considered a severe blunder. That is, I had not altered the image in any
way, other than to make minor adjustments to brightness, contrast and gamma
(i.e., adjustments the article would lead us to believe are relatively
innocent, and do not significantly alter the image's statement of
evidence). I actually agree with the committee's recommendations; so, what
did I do that was so wrong? I SAVED the file with the SAME filename! In
doing so, I had completely wiped pertinent information in the file format
that the SEM had written to the TIFF format. Such information would have
been important for duplicating the image, and under what circumstances the
image was acquired (e.g., keV, mag, tilt, detector, chamber pressure, etc
... in fact, a veritable wealth of information).

Like many of you, I was aware of the information, and should have known
better. It is now my practice for the SEM to write to a protected
directory. Relative to user retrieval, is now a read only directory. This
subject I expect the MSA sub-committee will address in a future report.

Regarding the reproducibility of image presentations, I also believe it's
worth mentioning several other issues that should have been addressed:

The first issue is that softwares, like Photoshop, are capable of writing
the history of modifications to the file (as well to a separate text file).
Actually, I am not aware of any other software with this capability, but the
possibility is an open standard for the TIFF file format. It is there for
any other software to implement. It may be that the MSA sub-committee
didn't want to suggest that we use a specific software, but I do believe
they should have mentioned the possibility, and that it would have been that
more "ethical" to use software that enabled the capability. Not to suggest
this method is perfect, but it is much easier than making the same entries
in your lab notes.

My last issue is a long and more complex rant, but it is connected to what
makes documenting the file's history possible. It is also about the
microscope specific information I mistakenly (however "innocently")
obliterated. That is, I don't know of a single SEM manufacturer who takes
advantage of the TIFF file format such that these accidents do not happen.
The problem (IMHO) is that SEM manufacturers take advantage of the
flexibility of the TIF format, but in a way that only they know how to read.
Some SEMs will simply append the data onto the end of the file, and others
will embed it in the header (examples included below). There is at least
one 3rd party software that knows how to read at least one SEM TIFF format,
but given the popularity, unique versatility, and availability of Photoshop
(and other softwares), this data should be standardized and written to the
TIFF such that it will remain safely. A case in point is the metadata, made
popular by today's digital cameras, that most (if not all) TIFF softwares
respect and will re-write to the TIFF file, including JPEGs. Furthermore,
Photoshop does not need to know it is there. Photoshop will simply
recognize the beginning and end of the XMP data, and re-write it when the
file is saved If XMP fields are created and standardized, it will make it
easy for microscopy softwares (and Photoshop compatible plug-ins) to find
and use.

Lastly ... I have no connection with Adobe relative to recommending their
products. I am sure I'm not alone in recognizing Adobe's contributions to
digital photography, supported by a huge user-base and many professionals.
Adobe also comes up with good ideas and provides a open forum and a means
for creating standards when no one else will. For concluding my rant in a
small way, can I suggest we expand this MSA subcommittee's mandate to look
into, ask this microscope community for suggestions for the types of data,
and follow the likes of NASA who created standards for embedding GPS
information in their image files.

genuinely :o)
michael shaffer

SEM/MLA Research Coordinator
(709) 737-6799 (ofc)
(709) 737-6790 (lab)
(709) 737-6193 (FAX)
{http://www.mun.ca/creait/maf/}
{http://www.esd.mun.ca/epma/}

Inco Innovation Centre
c/o Memorial University
230 Elizabeth Avenue
P.O. Box 4200
St. John's, NL A1C 5S7


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
FEI data (appended to end of file)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
[User]
User=XTLIB
Usertext=Quanta W
UsertextUnicode=5100750061006E007400610020005700
Date=03/31/2004
Time=01:29PM

[SYSTEM]
DNumber=D7500
Software=2.2
Source=W-Tetrode
Column=W-ESEM
FinalLens=W-ESEM
Chamber=
Stage=
Pump=TMP
ESEM=
Aperture=Fixed
Scan=dispb1.0
Acq=ViperQuad1.0
EucWD=0.01

[Beam]
HV=20000
Spot=6
StigmatorX=0.39061
StigmatorY=0.446543
BeamShiftX=0
BeamShiftY=0
ScanRotation=0
ImageMode=HR

[Scan]
InternalScan=true
Dwelltime=0.0001
FrameTime=94.251
PixelHeight=2.14827e-006
PixelWidth=2.14827e-006
Horfieldsize=0.00219983
Verfieldsize=0.00189907
Average=0
Integrate=0

[Stage]
StageX=0.00296175
StageY=0.00145804
StageZ=0.0100002
StageR=3.14107
StageT=-0.000855211
Spectilt=
WorkingDistance=0.00999534

[Vacuum]
UserMode=Highvacuum
CHPressure=90
Gas=

[Specimen]
Temperature=

[Detectors]
Number=1
Name=SSD

[SSD]
Contrast=72.9
Brightness=39
Signal=BSE
Mix=100
State=true
Active=true
Mode=0
ContrastDB=72.9
BrightnessDB=39
SegmentMode=0
Setting=A+B
ContrastSpotsizeAlignment=1
MinimumDetectorDwellTime=1e-006
[PrivateFei]
BitShift=0
Databarheight=0
DataBarSelected=HV Spot WD Sig Mag HFW MicronBar Label
TimeOfCreation=31.03.2004 13:41:17


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Zeiss data (embedded in header)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
4.157207e-006
8.409719e+001
6
2.000000e+004
2.660000e+000
2.080923e-010
8.303549e-003
1
4.157207e-006
8.409719e+001
6
2.000000e+004
2.660000e+000
2.080923e-010
8.303549e-003
2
4.157207e-006
8.409719e+001
6
2.000000e+004
2.660000e+000
2.080923e-010
8.303549e-003
3
4.157207e-006
8.409719e+001
6
2.000000e+004
2.660000e+000
2.080923e-010
8.303549e-003
36
AP_APERTURESIZE
Aperture Size = 100.0 µm
AP_BEAM_CURRENT
Beam Current = 100.0 µA
DP_RUNUPSTATE
Beam State = Beam On
AP_BEAM_TIME
Beam Time = 106.72 Hours
AP_BRIGHTNESS
Brightness = 48.3 %
AP_CHAMBER_PRESSURE
Chamber = 1.30e-003 Pa
AP_COLLECTOR_BIAS
Collector Bias = 250 V
AP_CONTRAST
Contrast = 26.6 %
AP_FRAME_TIME
Cycle Time = 40.3 Secs
AP_DATE
Date :11 Mar 2004
AP_ACTUALKV
EHT = 20.00 kV
DP_FIXED_APERTURE2
EP Aperture = None
DP_EP_MODE
EP Mode = Dry
AP_ACTUALCURRENT
Fil I = 2.660 A
AP_FILAMENT_AGE
Filament Age = 18.42 Hours
DP_FILAMENT_TYPE
Filament Type = W (Agar A054)
DP_FIXED_APERTURE
Fixed Aperture (VP) = Yes
AP_FRAME_AVERAGE_COUNT
Frames to average = 1
AP_FRAME_INT_COUNT
Frames to Int. = 0
AP_IPROBE
I Probe = 208 pA
AP_LINE_AVERAGE_COUNT
Line Avg.Count = 4
AP_LINE_INT_COUNT
Line int. count = 0
AP_MAG
Mag = 84 X
DP_OUT_DEV
Output dev = 19/21 inch display
DP_OUT_TYPE
Output To = Display/File
AP_HCSTAGE_TEMP
Peltier Temp = 20.0 °C
DP_PIXEL_SIZE
Pix Size state = calibrated
AP_PIXEL_SIZE
Pixel Size = 4.157 µm
DP_DETECTOR_CHANNEL
Signal A = SE1
DP_IMPLIED_DETECTOR
Signal B = SE1
AP_STAGE_AT_X
Stage at X = 54.327 mm
AP_STAGE_AT_Y
Stage at Y = 40.286 mm
AP_STAGE_AT_Z
Stage at Z = 15.481 mm
DP_USER
User = Busy
SV_USER_NAME
User Name = CAMKR
SV_USER_TEXT
User Text = text



==============================Original Headers==============================
24, 20 -- From Michael-at-Shaffer.net Mon Apr 10 11:22:12 2006
24, 20 -- Received: from ws6-3.us4.outblaze.com (ws6-3.us4.outblaze.com [205.158.62.199])
24, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k3AGM9d5031317
24, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 11:22:10 -0500
24, 20 -- Received: (qmail 20070 invoked from network); 10 Apr 2006 16:22:08 -0000
24, 20 -- Received: from unknown (HELO roamingwolf) (Michael-at-Shaffer.net-at-134.153.130.141)
24, 20 -- by ws6-3.us4.outblaze.com with SMTP; 10 Apr 2006 16:22:06 -0000
24, 20 -- From: "michael shaffer" {michael-at-Shaffer.net}
24, 20 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com}
24, 20 -- Subject: Ethics & Digital Imaging (long)
24, 20 -- Date: Mon, 10 Apr 2006 13:52:04 -0230
24, 20 -- Message-ID: {002001c65cba$e8d76320$b995fea9-at-roamingwolf}
24, 20 -- MIME-Version: 1.0
24, 20 -- Content-Type: text/plain;
24, 20 -- charset="iso-8859-1"
24, 20 -- X-Mailer: Microsoft Office Outlook 11
24, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
24, 20 -- Thread-Index: AcZcuuc5vNdzeoaRTj+XFLjeiFV89g==
24, 20 -- Content-Transfer-Encoding: 8bit
24, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3AGM9d5031317
==============================End of - Headers==============================




From: jbs-at-temple.edu
Date: Mon, 10 Apr 2006 13:20:13 -0500
Subject: [Microscopy] Re: Ethics & Digital Imaging (long)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Michael,

Your points are very well taken. It turns out that ImageJ has a
"macro recorder" that will track operations that are carried out on
images and, just like Photoshop, allow you to convert that into a
programmable routine. It is also possible to save this record as a
text file, which is, however, independent of the original image file.
This, of course, applies specifically to the post-processing
component of image generation.


}
}
}
} ----------------------------------------------------------------------
} ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} The other day I mistakenly destroyed relavent information regarding a
} SEM digital image. I instantly realized what I had done (... what a
} blunder!), and I should have known better. The mistake reminded me I
} had intended to read the article entitled "Ethics and Digital Imaging"
} (Microscopy Today, V14,N1, Jan06).
}
} The article's recommendations surprised me a bit relative to what I
} had considered a severe blunder. That is, I had not altered the
} image in any way, other than to make minor adjustments to brightness,
} contrast and gamma (i.e., adjustments the article would lead us to
} believe are relatively innocent, and do not significantly alter the
} image's statement of evidence). I actually agree with the committee's
} recommendations; so, what did I do that was so wrong? I SAVED the
} file with the SAME filename! In doing so, I had completely wiped
} pertinent information in the file format that the SEM had written to
} the TIFF format. Such information would have been important for
} duplicating the image, and under what circumstances the image was
} acquired (e.g., keV, mag, tilt, detector, chamber pressure, etc ... in
} fact, a veritable wealth of information).
}
} Like many of you, I was aware of the information, and should have
} known better. It is now my practice for the SEM to write to a
} protected directory. Relative to user retrieval, is now a read only
} directory. This subject I expect the MSA sub-committee will address
} in a future report.
}
} Regarding the reproducibility of image presentations, I also believe
} it's worth mentioning several other issues that should have been
} addressed:
}
} The first issue is that softwares, like Photoshop, are capable of
} writing the history of modifications to the file (as well to a
} separate text file). Actually, I am not aware of any other software
} with this capability, but the possibility is an open standard for the
} TIFF file format. It is there for any other software to implement.
} It may be that the MSA sub-committee didn't want to suggest that we
} use a specific software, but I do believe they should have mentioned
} the possibility, and that it would have been that more "ethical" to
} use software that enabled the capability. Not to suggest this method
} is perfect, but it is much easier than making the same entries in your
} lab notes.
}
} My last issue is a long and more complex rant, but it is connected to
} what makes documenting the file's history possible. It is also about
} the microscope specific information I mistakenly (however
} "innocently") obliterated. That is, I don't know of a single SEM
} manufacturer who takes advantage of the TIFF file format such that
} these accidents do not happen. The problem (IMHO) is that SEM
} manufacturers take advantage of the flexibility of the TIF format, but
} in a way that only they know how to read. Some SEMs will simply append
} the data onto the end of the file, and others will embed it in the
} header (examples included below). There is at least one 3rd party
} software that knows how to read at least one SEM TIFF format, but
} given the popularity, unique versatility, and availability of
} Photoshop (and other softwares), this data should be standardized and
} written to the TIFF such that it will remain safely. A case in point
} is the metadata, made popular by today's digital cameras, that most
} (if not all) TIFF softwares respect and will re-write to the TIFF
} file, including JPEGs. Furthermore, Photoshop does not need to know
} it is there. Photoshop will simply recognize the beginning and end of
} the XMP data, and re-write it when the file is saved If XMP fields
} are created and standardized, it will make it easy for microscopy
} softwares (and Photoshop compatible plug-ins) to find and use.
}
} Lastly ... I have no connection with Adobe relative to recommending
} their products. I am sure I'm not alone in recognizing Adobe's
} contributions to digital photography, supported by a huge user-base
} and many professionals. Adobe also comes up with good ideas and
} provides a open forum and a means for creating standards when no one
} else will. For concluding my rant in a small way, can I suggest we
} expand this MSA subcommittee's mandate to look into, ask this
} microscope community for suggestions for the types of data, and follow
} the likes of NASA who created standards for embedding GPS information
} in their image files.
}
} genuinely :o)
} michael shaffer
}
} SEM/MLA Research Coordinator
} (709) 737-6799 (ofc)
} (709) 737-6790 (lab)
} (709) 737-6193 (FAX)
} {http://www.mun.ca/creait/maf/}
} {http://www.esd.mun.ca/epma/}
}
} Inco Innovation Centre
} c/o Memorial University
} 230 Elizabeth Avenue
} P.O. Box 4200
} St. John's, NL A1C 5S7
}
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} FEI data (appended to end of file)
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} [User]
} User=XTLIB
} Usertext=Quanta W
} UsertextUnicode=5100750061006E007400610020005700
} Date=03/31/2004
} Time=01:29PM
}
} [SYSTEM]
} DNumber=D7500
} Software=2.2
} Source=W-Tetrode
} Column=W-ESEM
} FinalLens=W-ESEM
} Chamber=
} Stage=
} Pump=TMP
} ESEM=
} Aperture=Fixed
} Scan=dispb1.0
} Acq=ViperQuad1.0
} EucWD=0.01
}
} [Beam]
} HV=20000
} Spot=6
} StigmatorX=0.39061
} StigmatorY=0.446543
} BeamShiftX=0
} BeamShiftY=0
} ScanRotation=0
} ImageMode=HR
}
} [Scan]
} InternalScan=true
} Dwelltime=0.0001
} FrameTime=94.251
} PixelHeight=2.14827e-006
} PixelWidth=2.14827e-006
} Horfieldsize=0.00219983
} Verfieldsize=0.00189907
} Average=0
} Integrate=0
}
} [Stage]
} StageX=0.00296175
} StageY=0.00145804
} StageZ=0.0100002
} StageR=3.14107
} StageT=-0.000855211
} Spectilt=
} WorkingDistance=0.00999534
}
} [Vacuum]
} UserMode=Highvacuum
} CHPressure=90
} Gas=
}
} [Specimen]
} Temperature=
}
} [Detectors]
} Number=1
} Name=SSD
}
} [SSD]
} Contrast=72.9
} Brightness=39
} Signal=BSE
} Mix=100
} State=true
} Active=true
} Mode=0
} ContrastDB=72.9
} BrightnessDB=39
} SegmentMode=0
} Setting=A+B
} ContrastSpotsizeAlignment=1
} MinimumDetectorDwellTime=1e-006
} [PrivateFei]
} BitShift=0
} Databarheight=0
} DataBarSelected=HV Spot WD Sig Mag HFW MicronBar Label
} TimeOfCreation=31.03.2004 13:41:17
}
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Zeiss data (embedded in header)
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} 4.157207e-006
} 8.409719e+001
} 6
} 2.000000e+004
} 2.660000e+000
} 2.080923e-010
} 8.303549e-003
} 1
} 4.157207e-006
} 8.409719e+001
} 6
} 2.000000e+004
} 2.660000e+000
} 2.080923e-010
} 8.303549e-003
} 2
} 4.157207e-006
} 8.409719e+001
} 6
} 2.000000e+004
} 2.660000e+000
} 2.080923e-010
} 8.303549e-003
} 3
} 4.157207e-006
} 8.409719e+001
} 6
} 2.000000e+004
} 2.660000e+000
} 2.080923e-010
} 8.303549e-003
} 36
} AP_APERTURESIZE
} Aperture Size = 100.0 µm
} AP_BEAM_CURRENT
} Beam Current = 100.0 µA
} DP_RUNUPSTATE
} Beam State = Beam On
} AP_BEAM_TIME
} Beam Time = 106.72 Hours
} AP_BRIGHTNESS
} Brightness = 48.3 %
} AP_CHAMBER_PRESSURE
} Chamber = 1.30e-003 Pa
} AP_COLLECTOR_BIAS
} Collector Bias = 250 V
} AP_CONTRAST
} Contrast = 26.6 %
} AP_FRAME_TIME
} Cycle Time = 40.3 Secs
} AP_DATE
} Date :11 Mar 2004
} AP_ACTUALKV
} EHT = 20.00 kV
} DP_FIXED_APERTURE2
} EP Aperture = None
} DP_EP_MODE
} EP Mode = Dry
} AP_ACTUALCURRENT
} Fil I = 2.660 A
} AP_FILAMENT_AGE
} Filament Age = 18.42 Hours
} DP_FILAMENT_TYPE
} Filament Type = W (Agar A054)
} DP_FIXED_APERTURE
} Fixed Aperture (VP) = Yes
} AP_FRAME_AVERAGE_COUNT
} Frames to average = 1
} AP_FRAME_INT_COUNT
} Frames to Int. = 0
} AP_IPROBE
} I Probe = 208 pA
} AP_LINE_AVERAGE_COUNT
} Line Avg.Count = 4
} AP_LINE_INT_COUNT
} Line int. count = 0
} AP_MAG
} Mag = 84 X
} DP_OUT_DEV
} Output dev = 19/21 inch display
} DP_OUT_TYPE
} Output To = Display/File
} AP_HCSTAGE_TEMP
} Peltier Temp = 20.0 °C
} DP_PIXEL_SIZE
} Pix Size state = calibrated
} AP_PIXEL_SIZE
} Pixel Size = 4.157 µm
} DP_DETECTOR_CHANNEL
} Signal A = SE1
} DP_IMPLIED_DETECTOR
} Signal B = SE1
} AP_STAGE_AT_X
} Stage at X = 54.327 mm
} AP_STAGE_AT_Y
} Stage at Y = 40.286 mm
} AP_STAGE_AT_Z
} Stage at Z = 15.481 mm
} DP_USER
} User = Busy
} SV_USER_NAME
} User Name = CAMKR
} SV_USER_TEXT
} User Text = text
}
}
}
} ==============================Original
} Headers============================== 24, 20 -- From
} Michael-at-Shaffer.net Mon Apr 10 11:22:12 2006 24, 20 -- Received: from
} ws6-3.us4.outblaze.com (ws6-3.us4.outblaze.com [205.158.62.199]) 24,
} 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
} k3AGM9d5031317 24, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Apr
} 2006 11:22:10 -0500 24, 20 -- Received: (qmail 20070 invoked from
} network); 10 Apr 2006 16:22:08 -0000 24, 20 -- Received: from unknown
} (HELO roamingwolf) (Michael-at-Shaffer.net-at-134.153.130.141) 24, 20 --
} by ws6-3.us4.outblaze.com with SMTP; 10 Apr 2006 16:22:06 -0000 24, 20
} -- From: "michael shaffer" {michael-at-Shaffer.net} 24, 20 -- To: "MSA
} Microscopy list" {Microscopy-at-microscopy.com} 24, 20 -- Subject: Ethics
} & Digital Imaging (long) 24, 20 -- Date: Mon, 10 Apr 2006 13:52:04
} -0230 24, 20 -- Message-ID:
} {002001c65cba$e8d76320$b995fea9-at-roamingwolf} 24, 20 -- MIME-Version:
} 1.0 24, 20 -- Content-Type: text/plain; 24, 20 --
} charset="iso-8859-1" 24, 20 -- X-Mailer: Microsoft Office Outlook 11
} 24, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 24,
} 20 -- Thread-Index: AcZcuuc5vNdzeoaRTj+XFLjeiFV89g== 24, 20 --
} Content-Transfer-Encoding: 8bit 24, 20 -- X-MIME-Autoconverted: from
} quoted-printable to 8bit by ns.microscopy.com id k3AGM9d5031317
} ==============================End of -
} Headers==============================


Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs



==============================Original Headers==============================
8, 28 -- From jbs-at-temple.edu Mon Apr 10 13:20:13 2006
8, 28 -- Received: from po-smtp4.temple.edu (po-smtp4.temple.edu [155.247.166.232])
8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3AIKAUi010611
8, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 13:20:12 -0500
8, 28 -- Received: from [155.247.98.40] (jbs.bio.temple.edu [155.247.98.40])
8, 28 -- by po-smtp4.temple.edu (MOS 3.7.1-GA)
8, 28 -- with ESMTP id CPX28192 (AUTH jbs);
8, 28 -- Mon, 10 Apr 2006 14:19:58 -0400 (EDT)
8, 28 -- From: "Joel Sheffield" {jbs-at-temple.edu}
8, 28 -- To: michael-at-Shaffer.net, Microscopy-at-microscopy.com
8, 28 -- Date: Mon, 10 Apr 2006 14:22:17 -0400
8, 28 -- MIME-Version: 1.0
8, 28 -- Subject: Re: [Microscopy] Ethics & Digital Imaging (long)
8, 28 -- Reply-to: jbs-at-temple.edu
8, 28 -- Message-ID: {443A6A19.30006.6C074275-at-jbs.temple.edu}
8, 28 -- Priority: normal
8, 28 -- In-reply-to: {200604101622.k3AGMMHs031528-at-ns.microscopy.com}
8, 28 -- X-mailer: Pegasus Mail for Windows (4.30 public beta 1)
8, 28 -- Content-type: text/plain; charset=ISO-8859-1
8, 28 -- Content-description: Mail message body
8, 28 -- X-Junkmail-Status: score=10/50, host=po-smtp4.temple.edu
8, 28 -- X-Junkmail-SD-Raw: score=unknown,
8, 28 -- refid=str=0001.0A090207.443AA0AF.006F,ss=1,fgs=0,
8, 28 -- ip=155.247.98.40,
8, 28 -- so=2005-09-30 22:39:37,
8, 28 -- dmn=5.1.5/2006-02-08
8, 28 -- Content-Transfer-Encoding: 8bit
8, 28 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id k3AIKAUi010611
==============================End of - Headers==============================




From: michael-at-Shaffer.net
Date: Mon, 10 Apr 2006 13:50:19 -0500
Subject: [Microscopy] RE: Ethics & Digital Imaging (long)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Joel Sheffield writes ...

} Michael,
}
} Your points are very well taken. It turns out that ImageJ
} has a "macro recorder" that will track operations that are
} carried out on images and, just like Photoshop, allow you to
} convert that into a programmable routine. It is also
} possible to save this record as a text file, which is,
} however, independent of the original image file.

Yes ... There many possibilities, and I use ImageJ as well. With respect
to a macro however, what if you made a call to an automatic binary
thresholding routine? I dare say it would not save the threshold value that
it had automatically determined. Photoshop document "history" however will.
But, like I said, this implimentation isn't perfect either because it won't
report the history IF the operations were done via an PS action (...ARGH!...
And I had such high hopes!). It will however report the name of the action
used.

michael shaffer :o)

} ----------------------------------------------------------------------
} } ------ The Microscopy ListServer -- CoSponsor: The
} Microscopy Society
} } of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver On-Line Help



==============================Original Headers==============================
7, 21 -- From Michael-at-Shaffer.net Mon Apr 10 13:50:19 2006
7, 21 -- Received: from ws6-3.us4.outblaze.com (ws6-3.us4.outblaze.com [205.158.62.199])
7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k3AIoIrM021257
7, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 13:50:19 -0500
7, 21 -- Received: (qmail 30715 invoked from network); 10 Apr 2006 18:50:18 -0000
7, 21 -- Received: from unknown (HELO roamingwolf) (Michael-at-Shaffer.net-at-134.153.130.141)
7, 21 -- by ws6-3.us4.outblaze.com with SMTP; 10 Apr 2006 18:50:18 -0000
7, 21 -- From: "michael shaffer" {michael-at-Shaffer.net}
7, 21 -- To: {jbs-at-temple.edu} , {Microscopy-at-microscopy.com}
7, 21 -- Subject: RE: [Microscopy] Ethics & Digital Imaging (long)
7, 21 -- Date: Mon, 10 Apr 2006 16:20:15 -0230
7, 21 -- Message-ID: {002f01c65ccf$9c811a10$b995fea9-at-roamingwolf}
7, 21 -- MIME-Version: 1.0
7, 21 -- Content-Type: text/plain;
7, 21 -- charset="iso-8859-1"
7, 21 -- X-Mailer: Microsoft Office Outlook 11
7, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
7, 21 -- In-Reply-To: {443A6A19.30006.6C074275-at-jbs.temple.edu}
7, 21 -- Thread-Index: AcZcy2RyuKJ3mMPNSIaeE1mjdAbW2AAA0a1g
7, 21 -- Content-Transfer-Encoding: 8bit
7, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3AIoIrM021257
==============================End of - Headers==============================




From: Geoffrey_Williams-at-brown.edu
Date: Mon, 10 Apr 2006 15:03:26 -0500
Subject: [Microscopy] Ethics & Digital Imaging (long)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

My big problem/peeve, with TIFF files and microscope formats are not limited to SEM issues.

I don't buy the proprietary sales pitch anymore. Fixing the code to embed the data in the header is trivial and could be done by a competent programmer in an afternoon. Do we as a collective group have enough sway with the industry to get them to agree and write the files with collection information included? One problem for the Hitachi 2700 we've got would be it is a passive scan system and only records what ever data was on the screen. A simple prompt much like AMT's form field might go along way to getting the data into the header file that might other wise be lost/forgotten. A counter point would certainly be that Hitachi, JEOL, FEI for example use different terminology for the same parameters. Which would become standard, if we went to a standard (i.e.: probe current, beam current, spot size).

Like I said initially, the format is minor compared to the variety of bit depth issues. Sure you can use Metamorph, or ImagePro, or ImageJ to look at a 12 bit image, but if you take the same file to Photoshop it's a hit or miss if the way the Bit Depth is recorded is compatible. What I mean is that some like Olympus write the 12 bit images along the 16 bit scale and the software knows it is an Olympus file and displays it properly, but photoshop *can* open it, and once rescaled in 16 bit mode it can be viewed easily. Leica (and Zeiss I believe) chose to create a '12-bit' TIFF file that is actually a 16 bit file. Photoshop freaks out when it tries to open it. And by freaks out I mean it displays this: "Could not compete your request because the TIFF file uses an unsupported bit depth." If you delve into the header in Metamorph (which has the programming to make sense of the crazy file formats) it finds the "Bits per Sample = 16" and the following line defining "Used Bits per Sample = 12." Yes there are reasons and some suggest that these are the 'best' ways around dealing with a system running with 12 bit hardware and the constraints of a 16 bit system. Maybe I'm too much of a perfectionist to take that as the answer. But the subject cuts to the core of Ethics and Digital Imaging (IMHO - or IMnsHO).

With that said, I am on a personal crusade against 8 bit, in an age where memory and drive space is at an all time low cost and transfer rates and processing speeds are at an incredible high (go try and copy a 1 meg file off a floppy drive), why are files still being saved at a sub-optimal bit depth? If the system collects data at a 12 or 16 bit depth, why cut the arms and legs off the image and truncate it to 8? The quip of "it is easier to use the 8 bit data" is most irksome. Users with that retort frustrate me, because the systems we have in the facility, for the most part, have software that the users can load in limited capacity on their own systems and review and edit their images. But framed in light of the previous paragraph, there is a bit of a balancing act in the middle favoring 8-bit that supports the *user's quips.*

The header/system info is a minor issues compared to bit-depth and image information. At least with film the choice of levels was acceptable even at the lowest/cheapest level.

One the positive side, I have noticed an increasing move towards more consistent TIFF file formats. It isn't great but it is better than 'back in the day' when Photoshop 4.0 was hot off the floppy disks.

(thank you for the opportunity to vent on a current frustration)
Oh and I'll go ahead and plug my NESM colleague's symposium at Woods Hole on "The Ethics of Digital Imaging and Storage." I for one am eager to hear what the panelists will be saying!

Geoff Williams
Leduc Bioimaging Facility Manager
Brown University

http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/

-----Original Message-----
X-from: michael-at-Shaffer.net [mailto:michael-at-Shaffer.net]
Sent: Monday, April 10, 2006 12:26 PM
To: Williams, Geoffrey

The other day I mistakenly destroyed relavent information regarding a SEM
digital image. I instantly realized what I had done (... what a blunder!),
and I should have known better. The mistake reminded me I had intended to
read the article entitled "Ethics and Digital Imaging" (Microscopy Today,
V14,N1, Jan06).

The article's recommendations surprised me a bit relative to what I had
considered a severe blunder. That is, I had not altered the image in any
way, other than to make minor adjustments to brightness, contrast and gamma
(i.e., adjustments the article would lead us to believe are relatively
innocent, and do not significantly alter the image's statement of
evidence). I actually agree with the committee's recommendations; so, what
did I do that was so wrong? I SAVED the file with the SAME filename! In
doing so, I had completely wiped pertinent information in the file format
that the SEM had written to the TIFF format. Such information would have
been important for duplicating the image, and under what circumstances the
image was acquired (e.g., keV, mag, tilt, detector, chamber pressure, etc
... in fact, a veritable wealth of information).

Like many of you, I was aware of the information, and should have known
better. It is now my practice for the SEM to write to a protected
directory. Relative to user retrieval, is now a read only directory. This
subject I expect the MSA sub-committee will address in a future report.

Regarding the reproducibility of image presentations, I also believe it's
worth mentioning several other issues that should have been addressed:

The first issue is that softwares, like Photoshop, are capable of writing
the history of modifications to the file (as well to a separate text file).
Actually, I am not aware of any other software with this capability, but the
possibility is an open standard for the TIFF file format. It is there for
any other software to implement. It may be that the MSA sub-committee
didn't want to suggest that we use a specific software, but I do believe
they should have mentioned the possibility, and that it would have been that
more "ethical" to use software that enabled the capability. Not to suggest
this method is perfect, but it is much easier than making the same entries
in your lab notes.

My last issue is a long and more complex rant, but it is connected to what
makes documenting the file's history possible. It is also about the
microscope specific information I mistakenly (however "innocently")
obliterated. That is, I don't know of a single SEM manufacturer who takes
advantage of the TIFF file format such that these accidents do not happen.
The problem (IMHO) is that SEM manufacturers take advantage of the
flexibility of the TIF format, but in a way that only they know how to read.
Some SEMs will simply append the data onto the end of the file, and others
will embed it in the header (examples included below). There is at least
one 3rd party software that knows how to read at least one SEM TIFF format,
but given the popularity, unique versatility, and availability of Photoshop
(and other softwares), this data should be standardized and written to the
TIFF such that it will remain safely. A case in point is the metadata, made
popular by today's digital cameras, that most (if not all) TIFF softwares
respect and will re-write to the TIFF file, including JPEGs. Furthermore,
Photoshop does not need to know it is there. Photoshop will simply
recognize the beginning and end of the XMP data, and re-write it when the
file is saved If XMP fields are created and standardized, it will make it
easy for microscopy softwares (and Photoshop compatible plug-ins) to find
and use.

Lastly ... I have no connection with Adobe relative to recommending their
products. I am sure I'm not alone in recognizing Adobe's contributions to
digital photography, supported by a huge user-base and many professionals.
Adobe also comes up with good ideas and provides a open forum and a means
for creating standards when no one else will. For concluding my rant in a
small way, can I suggest we expand this MSA subcommittee's mandate to look
into, ask this microscope community for suggestions for the types of data,
and follow the likes of NASA who created standards for embedding GPS
information in their image files.

genuinely :o)
michael shaffer

SEM/MLA Research Coordinator
(709) 737-6799 (ofc)
(709) 737-6790 (lab)
(709) 737-6193 (FAX)
{http://www.mun.ca/creait/maf/}
{http://www.esd.mun.ca/epma/}

Inco Innovation Centre
c/o Memorial University
230 Elizabeth Avenue
P.O. Box 4200
St. John's, NL A1C 5S7


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
FEI data (appended to end of file)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
[User]
User=XTLIB
Usertext=Quanta W
UsertextUnicode=5100750061006E007400610020005700
Date=03/31/2004
Time=01:29PM

[SYSTEM]
DNumber=D7500
Software=2.2
Source=W-Tetrode
Column=W-ESEM
FinalLens=W-ESEM
Chamber=
Stage=
Pump=TMP
ESEM=
Aperture=Fixed
Scan=dispb1.0
Acq=ViperQuad1.0
EucWD=0.01

[Beam]
HV=20000
Spot=6
StigmatorX=0.39061
StigmatorY=0.446543
BeamShiftX=0
BeamShiftY=0
ScanRotation=0
ImageMode=HR

[Scan]
InternalScan=true
Dwelltime=0.0001
FrameTime=94.251
PixelHeight=2.14827e-006
PixelWidth=2.14827e-006
Horfieldsize=0.00219983
Verfieldsize=0.00189907
Average=0
Integrate=0

[Stage]
StageX=0.00296175
StageY=0.00145804
StageZ=0.0100002
StageR=3.14107
StageT=-0.000855211
Spectilt=
WorkingDistance=0.00999534

[Vacuum]
UserMode=Highvacuum
CHPressure=90
Gas=

[Specimen]
Temperature=

[Detectors]
Number=1
Name=SSD

[SSD]
Contrast=72.9
Brightness=39
Signal=BSE
Mix=100
State=true
Active=true
Mode=0
ContrastDB=72.9
BrightnessDB=39
SegmentMode=0
Setting=A+B
ContrastSpotsizeAlignment=1
MinimumDetectorDwellTime=1e-006
[PrivateFei]
BitShift=0
Databarheight=0
DataBarSelected=HV Spot WD Sig Mag HFW MicronBar Label
TimeOfCreation=31.03.2004 13:41:17


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Zeiss data (embedded in header)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
4.157207e-006
8.409719e+001
6
2.000000e+004
2.660000e+000
2.080923e-010
8.303549e-003
1
4.157207e-006
8.409719e+001
6
2.000000e+004
2.660000e+000
2.080923e-010
8.303549e-003
2
4.157207e-006
8.409719e+001
6
2.000000e+004
2.660000e+000
2.080923e-010
8.303549e-003
3
4.157207e-006
8.409719e+001
6
2.000000e+004
2.660000e+000
2.080923e-010
8.303549e-003
36
AP_APERTURESIZE
Aperture Size = 100.0 µm
AP_BEAM_CURRENT
Beam Current = 100.0 µA
DP_RUNUPSTATE
Beam State = Beam On
AP_BEAM_TIME
Beam Time = 106.72 Hours
AP_BRIGHTNESS
Brightness = 48.3 %
AP_CHAMBER_PRESSURE
Chamber = 1.30e-003 Pa
AP_COLLECTOR_BIAS
Collector Bias = 250 V
AP_CONTRAST
Contrast = 26.6 %
AP_FRAME_TIME
Cycle Time = 40.3 Secs
AP_DATE
Date :11 Mar 2004
AP_ACTUALKV
EHT = 20.00 kV
DP_FIXED_APERTURE2
EP Aperture = None
DP_EP_MODE
EP Mode = Dry
AP_ACTUALCURRENT
Fil I = 2.660 A
AP_FILAMENT_AGE
Filament Age = 18.42 Hours
DP_FILAMENT_TYPE
Filament Type = W (Agar A054)
DP_FIXED_APERTURE
Fixed Aperture (VP) = Yes
AP_FRAME_AVERAGE_COUNT
Frames to average = 1
AP_FRAME_INT_COUNT
Frames to Int. = 0
AP_IPROBE
I Probe = 208 pA
AP_LINE_AVERAGE_COUNT
Line Avg.Count = 4
AP_LINE_INT_COUNT
Line int. count = 0
AP_MAG
Mag = 84 X
DP_OUT_DEV
Output dev = 19/21 inch display
DP_OUT_TYPE
Output To = Display/File
AP_HCSTAGE_TEMP
Peltier Temp = 20.0 °C
DP_PIXEL_SIZE
Pix Size state = calibrated
AP_PIXEL_SIZE
Pixel Size = 4.157 µm
DP_DETECTOR_CHANNEL
Signal A = SE1
DP_IMPLIED_DETECTOR
Signal B = SE1
AP_STAGE_AT_X
Stage at X = 54.327 mm
AP_STAGE_AT_Y
Stage at Y = 40.286 mm
AP_STAGE_AT_Z
Stage at Z = 15.481 mm
DP_USER
User = Busy
SV_USER_NAME
User Name = CAMKR
SV_USER_TEXT
User Text = text



==============================Original Headers==============================
24, 20 -- From Michael-at-Shaffer.net Mon Apr 10 11:22:12 2006
24, 20 -- Received: from ws6-3.us4.outblaze.com (ws6-3.us4.outblaze.com [205.158.62.199])
24, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k3AGM9d5031317
24, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 11:22:10 -0500
24, 20 -- Received: (qmail 20070 invoked from network); 10 Apr 2006 16:22:08 -0000
24, 20 -- Received: from unknown (HELO roamingwolf) (Michael-at-Shaffer.net-at-134.153.130.141)
24, 20 -- by ws6-3.us4.outblaze.com with SMTP; 10 Apr 2006 16:22:06 -0000
24, 20 -- From: "michael shaffer" {michael-at-Shaffer.net}
24, 20 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com}
24, 20 -- Subject: Ethics & Digital Imaging (long)
24, 20 -- Date: Mon, 10 Apr 2006 13:52:04 -0230
24, 20 -- Message-ID: {002001c65cba$e8d76320$b995fea9-at-roamingwolf}
24, 20 -- MIME-Version: 1.0
24, 20 -- Content-Type: text/plain;
24, 20 -- charset="iso-8859-1"
24, 20 -- X-Mailer: Microsoft Office Outlook 11
24, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
24, 20 -- Thread-Index: AcZcuuc5vNdzeoaRTj+XFLjeiFV89g==
24, 20 -- Content-Transfer-Encoding: 8bit
24, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3AGM9d5031317
==============================End of - Headers==============================


==============================Original Headers==============================
38, 29 -- From Geoffrey_Williams-at-brown.edu Mon Apr 10 15:03:25 2006
38, 29 -- Received: from pyxis.services.brown.edu (pyxis.services.brown.edu [128.148.106.154])
38, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3AK3Ppd000699
38, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 15:03:25 -0500
38, 29 -- Received: from ex-gateway2-out.AD.Brown.Edu (ex-gateway2.ad.brown.edu [128.148.21.53])
38, 29 -- by pyxis.services.brown.edu (Switch-3.1.8/Switch-3.1.7/) with ESMTP id k3AK3LoB023648
38, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 16:03:21 -0400 (EDT)
38, 29 -- Received: from mail1.AD.Brown.Edu ([128.148.21.31]) by ex-gateway2-out.AD.Brown.Edu with Microsoft SMTPSVC(6.0.3790.1830);
38, 29 -- Mon, 10 Apr 2006 16:03:21 -0400
38, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0
38, 29 -- Content-class: urn:content-classes:message
38, 29 -- MIME-Version: 1.0
38, 29 -- Content-Type: text/plain;
38, 29 -- charset="iso-8859-1"
38, 29 -- Subject: RE: [Microscopy] Ethics & Digital Imaging (long) (mine reply is a bit long too)
38, 29 -- Date: Mon, 10 Apr 2006 16:03:20 -0400
38, 29 -- Message-ID: {A1A84D541C161C4C988B4E0FB38F5A0F0433378E-at-MAIL1.AD.Brown.Edu}
38, 29 -- X-MS-Has-Attach:
38, 29 -- X-MS-TNEF-Correlator:
38, 29 -- Thread-Topic: [Microscopy] Ethics & Digital Imaging (long) (mine reply is a bit long too)
38, 29 -- Thread-Index: AcZcu4NHTPkBWxjxSlipd8DyO4umMgAGN24w
38, 29 -- From: "Williams, Geoffrey" {Geoffrey_Williams-at-brown.edu}
38, 29 -- To: {Microscopy-at-microscopy.com}
38, 29 -- X-OriginalArrivalTime: 10 Apr 2006 20:03:21.0678 (UTC) FILETIME=[D15C3EE0:01C65CD9]
38, 29 -- X-Brown-Proofpoint: Not Infected
38, 29 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 mlx=-1 adultscore=0 adjust=0 reason=safe engine=3.1.0-06040600 definitions=3.0.0-06041008
38, 29 -- X-Brown-MailScanner-SpamCheck: not spam, rule=notspam policy=default score=0 mlx=-1 adultscore=0 adjust=0 reason=safe engine=3.1.0-06040600 definitions=3.0.0-06041008
38, 29 -- Content-Transfer-Encoding: 8bit
38, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3AK3Ppd000699
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Mon, 10 Apr 2006 15:31:52 -0500
Subject: [Microscopy] Fluorescence/SEM correlative microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Ah, Mondays.

Does anyone have a favorite method by which the same cell in a culture
could be imaged by fluorescence microscopy and SEM? I'm scanning the
catalogs and databases for etched reference cover slips, etc., but maybe
someone has "the magic wand"?

Grateful as usual for any help!

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







==============================Original Headers==============================
9, 23 -- From TindallR-at-missouri.edu Mon Apr 10 15:31:51 2006
9, 23 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49])
9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3AKVoVX011025
9, 23 -- for {microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 15:31:51 -0500
9, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830);
9, 23 -- Mon, 10 Apr 2006 15:31:49 -0500
9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 23 -- Content-class: urn:content-classes:message
9, 23 -- MIME-Version: 1.0
9, 23 -- Content-Type: text/plain;
9, 23 -- charset="us-ascii"
9, 23 -- Subject: Fluorescence/SEM correlative microscopy
9, 23 -- Date: Mon, 10 Apr 2006 15:31:48 -0500
9, 23 -- Message-ID: {BA876152E8653240BE8572E897083EE701849ECE-at-UM-EMAIL09.um.umsystem.edu}
9, 23 -- X-MS-Has-Attach:
9, 23 -- X-MS-TNEF-Correlator:
9, 23 -- Thread-Topic: Fluorescence/SEM correlative microscopy
9, 23 -- thread-index: AcZc3cqML7w+B/piQpSnZR9KLyIx9w==
9, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
9, 23 -- To: {microscopy-at-microscopy.com}
9, 23 -- X-OriginalArrivalTime: 10 Apr 2006 20:31:49.0238 (UTC) FILETIME=[CB253960:01C65CDD]
9, 23 -- Content-Transfer-Encoding: 8bit
9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3AKVoVX011025
==============================End of - Headers==============================




From: pedromfjcosta-at-gmail.com
Date: Mon, 10 Apr 2006 23:44:01 -0500
Subject: [Microscopy] viaWWW: Tripod Polisher micrometers locking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both pedromfjcosta-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: pedromfjcosta-at-gmail.com
Name: Pedro MFJ Costa

Organization: University of Cambridge

Title-Subject: [Filtered] Tripod Polisher micrometers locking

Question: We have several tripod polishers in your lab and, unfortunately, we find that with time the precision micrometers tend to get locked up. In fact, despite trying to avoid excessive water infiltrating the micrometers they still become rusty.

I was wondering if anyone had a way to prevent this from happening or possibly to unlock them without incurring into further damage.


---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Mon Apr 10 23:44:01 2006
8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3B4i0Dm027217
8, 12 -- for {microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 23:44:00 -0500
8, 12 -- Mime-Version: 1.0
8, 12 -- X-Sender: (Unverified)
8, 12 -- Message-Id: {p06110403c060e47f14e0-at-[206.69.208.22]}
8, 12 -- Date: Mon, 10 Apr 2006 23:43:57 -0500
8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: pedromfjcosta-at-gmail.com (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: Tripod Polisher micrometers locking
8, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: coss.eps-at-ceu.es
Date: Tue, 11 Apr 2006 02:19:58 -0500
Subject: [Microscopy] Summerschool on Advanced data analysis and modelling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

San Pablo - CEU University in collaboration with other five universities
(Málaga, Politécnica de Madrid, País Vasco, Rey Juan Carlos, and
Castilla La Mancha), nine companies, CSIC and IEEE organizes a
summerschool on "Advanced Data Analysis and Modeling" in Madrid between
June 26th and July 27th. The full summerschool is 120 hours long and is
divided into 10 courses. Attendees may register in each course
independently. The deadline for registration is June 1st. For more
information, please, visit
http://biocomp.cnb.csic.es/~coss/Docencia/ADAM/ADAM.htm

Best regards, Carlos Oscar

*List of courses and brief description* (full description at
http://biocomp.cnb.csic.es/~coss/Docencia/ADAM/ADAM.htm)

Course 1. STATISTICAL INFERENCE (June 26th - June 29th)
Introduction, Some basic statistical tests, Simple linear regression.
Practical sessions: SPSS
Course 2. MULTIVARIATE DATA ANALYSIS (June 26th - June 29th)
Introduction, Data examination, Factor analysis, MANOVA,
Multidimensional scaling, Structural equation modeling. Practical
sessions: SPSS
Course 3. BAYESIAN NETWORKS (July 3rd - July 6th)
Basics, Inference in Bayesian networks, Learning Bayesian networks
from data. Practical sessions: Hugin, Elvira, Weka, LibB
Course 4. NEURAL NETWORKS (July 3rd - July 6th)
Introduction, Perceptron networks, The Hebb rule, Multivariate
optimization, Rule of Widrow-Hoff, Backpropagation. Practical sessions:
MATLAB
Course 5. ASSOCIATION RULES (July 10th - July 13th)
Introduction, Rule discovering, Knowledge discoverage in biological
data, Applications. Practical sessions: Bioinformatics tools
Course 6. EXPERT SYSTEMS (July 10th - July 13th)
Introduction, Expert system programming, Hybrid systems. Practical
sessions: CLIPS and JESS
Course 7. HIDDEN MARKOV MODELS (July 17th - July 20th)
Introduction, Discrete HMM, Basic algorithms, Semicontinuous HMMs,
Continuous HMMs, Clustering, Generalized HMMs. Practical sessions: HTK
Course 8. TIME SERIES ANALYSIS (July 17th - July 20th)
Introduction. Probability models, Regression and Fourier analysis,
Forecasting and Data mining. Practical sessions: MATLAB, R.
Course 9. DATA MINING (July 24th - July 27th)
Introduction, Exploring data, Classification, Cluster analysis,
Survival analysis, Anomaly detection. Practical sessions: R, WEKA
Course 10. PATTERN RECOGNITION (July 24th - July 27th)
Introduction, Performance of supervised classification, Preprocessing,
k-nearest neighbor, classification trees, logistic regression, rule
induction, combining classifiers, unsupervised classification. Practical
sessions: WEKA

--
-----------------------------------------------------------
Carlos Óscar Sánchez Sorzano coss.eps-at-ceu.es
Escuela Politécnica Superior Tel:+34 91 372 4034
Univ. San Pablo - CEU Fax:+34 91 372 4049
Campus Urb. Montepríncipe s/n
28668 Boadilla del Monte - Madrid http://www.uspceu.com
Spain
-----------------------------------------------------------




______________________________________________
LLama Gratis a cualquier PC del Mundo.
Llamadas a fijos y móviles desde 1 céntimo por minuto.
http://es.voice.yahoo.com

==============================Original Headers==============================
9, 17 -- From coss.eps-at-ceu.es Tue Apr 11 02:19:58 2006
9, 17 -- Received: from smtp106.plus.mail.mud.yahoo.com (smtp106.plus.mail.mud.yahoo.com [68.142.206.239])
9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k3B7Jwq5007236
9, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 11 Apr 2006 02:19:58 -0500
9, 17 -- Received: (qmail 21169 invoked from network); 11 Apr 2006 07:19:57 -0000
9, 17 -- Received: from unknown (HELO ?127.0.0.1?) (cos?sorzano-at-62.14.112.193 with plain)
9, 17 -- by smtp106.plus.mail.mud.yahoo.com with SMTP; 11 Apr 2006 07:19:57 -0000
9, 17 -- Message-ID: {443B595C.7070503-at-ceu.es}
9, 17 -- Date: Tue, 11 Apr 2006 09:23:08 +0200
9, 17 -- From: =?ISO-8859-1?Q?Carlos_Oscar_S=E1nchez_Sorzano?= {coss.eps-at-ceu.es}
9, 17 -- User-Agent: Mozilla Thunderbird 1.0.7 (Windows/20050923)
9, 17 -- X-Accept-Language: en-us, en
9, 17 -- MIME-Version: 1.0
9, 17 -- To: Microscopy-at-Microscopy.Com
9, 17 -- Subject: Summerschool on Advanced data analysis and modelling
9, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
9, 17 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: protrain-at-emcourses.com
Date: Tue, 11 Apr 2006 05:30:00 -0500
Subject: [Microscopy] Digital Imaging in the SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi All

World famous for throwing in a grenade (just ask my friends) it never ceases
to fascinate me of how popular the processing of SEM images seems to be. Go
to a conference and it is only the person demonstrating Photoshop who
manages to fill the lecture theatre!

Have we forgotten how to use the SEM, use the features correctly and
optimise the photographic/imaging contrast and brightness? In the days when
some of my clients produced 15,000 Polaroid pictures per year 90% of then
looked pretty good as they came off the machine, it seems that today 50%
have to be "improved" by artificial means?

Are we substituting talented computer operators for talented SEM operators,
it does not seem that we are scientists in microscopy any more?

Think on :)

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7711 606967 Web www.emcourses.com


==============================Original Headers==============================
7, 29 -- From protrain-at-emcourses.com Tue Apr 11 05:30:00 2006
7, 29 -- Received: from smtp01.x-mailer.co.uk (smtp01.x-mailer.co.uk [195.13.65.37])
7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3BAU0VE019274
7, 29 -- for {microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 05:30:00 -0500
7, 29 -- Received: from [62.69.65.169] (helo=smtp-f.mail.legend.co.uk)
7, 29 -- by smtp01.x-mailer.co.uk with smtp (Exim 4.60)
7, 29 -- (envelope-from {protrain-at-emcourses.com} )
7, 29 -- id 1FTG8E-0006LI-VV
7, 29 -- for microscopy-at-microscopy.com; Tue, 11 Apr 2006 11:29:59 +0100
7, 29 -- Received: (qmail 12648 invoked from network); 11 Apr 2006 10:29:48 -0000
7, 29 -- Received: from unknown (HELO advent) (212.248.148.141)
7, 29 -- by 0 with SMTP; 11 Apr 2006 10:29:48 -0000
7, 29 -- Message-ID: {00a801c65d53$50db83f0$3493f8d4-at-advent}
7, 29 -- Reply-To: "Steve Chapman" {protrain-at-emcourses.com}
7, 29 -- From: "Steve Chapman" {protrain-at-emcourses.com}
7, 29 -- To: "American Soc" {microscopy-at-microscopy.com}
7, 29 -- Subject: Digital Imaging in the SEM
7, 29 -- Date: Tue, 11 Apr 2006 10:42:37 +0100
7, 29 -- Organization: Protrain
7, 29 -- MIME-Version: 1.0
7, 29 -- Content-Type: text/plain;
7, 29 -- format=flowed;
7, 29 -- charset="iso-8859-1";
7, 29 -- reply-type=original
7, 29 -- Content-Transfer-Encoding: 7bit
7, 29 -- X-Priority: 3
7, 29 -- X-MSMail-Priority: Normal
7, 29 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2527
7, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2527
==============================End of - Headers==============================




From: frank.karl-at-degussa.com
Date: Tue, 11 Apr 2006 07:09:46 -0500
Subject: [Microscopy] Re: Digital Imaging in the SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

"Have we forgotten how to use the SEM, use the features correctly and
optimize the photographic/imaging contrast and brightness? In the days
when
some of my clients produced 15,000 Polaroid pictures per year 90% of then
looked pretty good as they came off the machine, it seems that today 50%
have to be "improved" by artificial means?

Are we substituting talented computer operators for talented SEM operators,

it does not seem that we are scientists in microscopy any more?"

Let me help you pull the pin........

When I studied photomicrography my instructor, John Delly, use to tell me
that a photomicrograph needs to be more than correctly exposed and
illuminated. More than simply demonstrating the feature or reason for the
exposure, each photograph it needs to be composed. It needs to be artistic
so the viewer wants to view the image. He admitted that not every
photograph will be a little artistic masterpiece, but an effort needs to be
made in every exposure. I never felt there should be a difference in this
approach with either light or electron images.

So the question is, if I compose, correctly expose, balance contrast and
illumination levels, do I Photoshop just to Photoshop? My personal answer
is that (assume correct documentation) if Photoshop, color washes, false
color, unsharpmask makes a feature which already exist easier to see or
understand, why not? My "client" need to understand what I see and
perceive. Did we not use different developers to produce different prints,
paste arrows on photos, hand tint, dot map with colored filters to enhance
the information and its understanding?

Few of us have the ability to ring slides, make our own polarizing filters
or weld filaments to posts or mix refractive index liquids, yet these were
skills once needed. We have moved on, so it will be with imaging.

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
messages attached to it may contain information that is confidential or
legally privileged. If you are not the intended recipient, or a person
responsible for delivering it to the intended recipient, you are hereby
notified that you must not read this transmission and that any disclosure,
copying, printing, distribution or use of any of the information contained
in or attached to this transmission is STRICTLY PROHIBITED. If you have
received this transmission in error, please immediately notify the sender
by telephone or return e-mail and delete the original transmission and its
attachments without reading or saving in any manner. Thank you.



protrain-at-emcourse
s.com To: frank.karl-at-degussa.com
cc:
04/11/2006 06:32 Subject: [Microscopy] Digital Imaging in the SEM
AM
Please respond to
protrain








----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Hi All

World famous for throwing in a grenade (just ask my friends) it never
ceases
to fascinate me of how popular the processing of SEM images seems to be.
Go
to a conference and it is only the person demonstrating Photoshop who
manages to fill the lecture theatre!



Think on :)

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7711 606967 Web www.emcourses.com


==============================Original
Headers==============================
7, 29 -- From protrain-at-emcourses.com Tue Apr 11 05:30:00 2006
7, 29 -- Received: from smtp01.x-mailer.co.uk (smtp01.x-mailer.co.uk
[195.13.65.37])
7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id k3BAU0VE019274
7, 29 -- for {microscopy-at-microscopy.com} ; Tue, 11 Apr 2006
05:30:00 -0500
7, 29 -- Received: from [62.69.65.169] (helo=smtp-f.mail.legend.co.uk)
7, 29 -- by smtp01.x-mailer.co.uk with smtp (Exim 4.60)
7, 29 -- (envelope-from {protrain-at-emcourses.com} )
7, 29 -- id 1FTG8E-0006LI-VV
7, 29 -- for microscopy-at-microscopy.com; Tue, 11 Apr 2006 11:29:59
+0100
7, 29 -- Received: (qmail 12648 invoked from network); 11 Apr 2006 10:29:48
-0000
7, 29 -- Received: from unknown (HELO advent) (212.248.148.141)
7, 29 -- by 0 with SMTP; 11 Apr 2006 10:29:48 -0000
7, 29 -- Message-ID: {00a801c65d53$50db83f0$3493f8d4-at-advent}
7, 29 -- Reply-To: "Steve Chapman" {protrain-at-emcourses.com}
7, 29 -- From: "Steve Chapman" {protrain-at-emcourses.com}
7, 29 -- To: "American Soc" {microscopy-at-microscopy.com}
7, 29 -- Subject: Digital Imaging in the SEM
7, 29 -- Date: Tue, 11 Apr 2006 10:42:37 +0100
7, 29 -- Organization: Protrain
7, 29 -- MIME-Version: 1.0
7, 29 -- Content-Type: text/plain;
7, 29 -- format=flowed;
7, 29 -- charset="iso-8859-1";
7, 29 -- reply-type=original
7, 29 -- Content-Transfer-Encoding: 7bit
7, 29 -- X-Priority: 3
7, 29 -- X-MSMail-Priority: Normal
7, 29 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2527
7, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2527
==============================End of -
Headers==============================




==============================Original Headers==============================
34, 18 -- From frank.karl-at-degussa.com Tue Apr 11 07:09:45 2006
34, 18 -- Received: from malmailout1.rz.itson.com (mailout1.degussa.com [193.100.56.173])
34, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3BC9hww030494
34, 18 -- for {microscopy-at-msa.microscopy.com} ; Tue, 11 Apr 2006 07:09:44 -0500
34, 18 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [172.20.6.74])
34, 18 -- by malmailout1.rz.itson.com (8.13.3/8.13.3/Debian-6) with SMTP id k3BC9agD001475;
34, 18 -- Tue, 11 Apr 2006 14:09:37 +0200
34, 18 -- In-Reply-To: {200604111032.k3BAWZIW021656-at-ns.microscopy.com}
34, 18 -- Subject: Re: [Microscopy] Digital Imaging in the SEM
34, 18 -- To: protrain-at-emcourses.com, microscopy-at-msa.microscopy.com
34, 18 -- X-Mailer: Lotus Notes Release 6.5.2 June 01, 2004
34, 18 -- Message-ID: {OF5D29A84A.80CBEE9B-ON8525714D.0040ADD7-8525714D.0042C5AE-at-degussa.com}
34, 18 -- From: frank.karl-at-degussa.com
34, 18 -- Date: Tue, 11 Apr 2006 08:09:19 -0400
34, 18 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at
34, 18 -- 04/11/2006 07:09:38 AM
34, 18 -- MIME-Version: 1.0
34, 18 -- Content-type: text/plain; charset=US-ASCII
==============================End of - Headers==============================




From: jrunions-at-brookes.ac.uk
Date: Tue, 11 Apr 2006 07:54:11 -0500
Subject: [Microscopy] Re: Digital Imaging in the SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


In agreement with what Frank says, a photoshop guru will tell you,
'Photoshop can make a good image better, but it can't make a bad image
good.' I find this to be true and I think we still need to instruct in
the art of exposure and composition. Ultimately, using Photoshop to
adjust levels is the same as using filters and dodging and burning in
the old days (5 years ago). What I don't understand is where all the
free time that was once spent in the darkroom has gone...

John.

frank.karl-at-degussa.com wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

--

*********************************
C. John Runions, Ph.D.
School of Biological and Molecular Sciences
Oxford Brookes University
Oxford, UK
OX3 0BP

email: jrunions-at-brookes.ac.uk
phone: +44 (0) 1865 483 964


==============================Original Headers==============================
9, 22 -- From jrunions-at-brookes.ac.uk Tue Apr 11 07:54:11 2006
9, 22 -- Received: from brookes.ac.uk (csmail1.brookes.ac.uk [161.73.1.23])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3BCsA80008421
9, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 11 Apr 2006 07:54:10 -0500
9, 22 -- Received: from [161.73.107.141] (bc8bf582.brookes.ac.uk [161.73.107.141])
9, 22 -- by brookes.ac.uk (8.13.6/8.13.6) with ESMTP id k3BCoYlM019225
9, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 11 Apr 2006 13:50:34 +0100 (BST)
9, 22 -- Message-ID: {443BA61B.6090906-at-brookes.ac.uk}
9, 22 -- Date: Tue, 11 Apr 2006 13:50:35 +0100
9, 22 -- From: John Runions {jrunions-at-brookes.ac.uk}
9, 22 -- User-Agent: Mozilla Thunderbird 1.0 (Windows/20041206)
9, 22 -- X-Accept-Language: en-us, en
9, 22 -- MIME-Version: 1.0
9, 22 -- To: Microscopy-at-Microscopy.Com
9, 22 -- Subject: Re: Digital Imaging in the SEM
9, 22 -- References: {200604111213.k3BCDrRF005518-at-ns.microscopy.com}
9, 22 -- In-Reply-To: {200604111213.k3BCDrRF005518-at-ns.microscopy.com}
9, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
9, 22 -- Content-Transfer-Encoding: 7bit
9, 22 -- X-MailScanner-Information: Oxford Brookes University MailScanner
9, 22 -- X-MailScanner: Clean
9, 22 -- X-MailScanner-From: jrunions-at-brookes.ac.uk
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Tue, 11 Apr 2006 08:57:13 -0500
Subject: [Microscopy] Digital Imaging in the SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

As someone who has spent a substantial portion of my life since age 12
in photographic darkrooms, as well as studying and admiring the works
and methods of the "masters" of photography, it has been my perception
that few prints make it out of the darkroom without being manipulated.
This applies, in my experience, to artistic as well as technical images.
There were a few master photographers who practiced "straight"
photography, whatever that means (there are NO unmanipulated images),
but wasn't it Ansel himself who compared a negative to a musical score
to be interpreted by the conductor-printer? At least I think it was old
Ansel. The Westons dodged and burned and manipulated freely, and it
certainly wasn't for lack of techical expertise in operating their
cameras and processing their films.

In my new, little has changed except the tools. We now use pixels and
electrons, where we once danced around the darkroom doing elaborate jigs
to cut a little light here and burn a highlight there, drawing complex
diagrams of how many seconds this area gets, what contrast filter
(gasp!!!! contrast filters!!!) that area gets, etc., etc., etc. In
the process, we went through uncounted sheets of expensive, silver-laden
paper and flushed untold gallons of nasty chemicals down the drain.

I miss all that sometimes, because there was something really peaceful
and fulfilling about producing a fine silver print, especially with the
soothing tones of Metallica playing in the background. But, I feel I
have better control now, with less waste and time, with often superior
results. If I ever get back into the darkroom, it will be as a hobby.

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







==============================Original Headers==============================
9, 23 -- From TindallR-at-missouri.edu Tue Apr 11 08:57:12 2006
9, 23 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49])
9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3BDvB7P019836
9, 23 -- for {microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 08:57:12 -0500
9, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830);
9, 23 -- Tue, 11 Apr 2006 08:57:10 -0500
9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
9, 23 -- Content-class: urn:content-classes:message
9, 23 -- MIME-Version: 1.0
9, 23 -- Content-Type: text/plain;
9, 23 -- charset="us-ascii"
9, 23 -- Subject: Digital Imaging in the SEM
9, 23 -- Date: Tue, 11 Apr 2006 08:57:09 -0500
9, 23 -- Message-ID: {BA876152E8653240BE8572E897083EE701849ED4-at-UM-EMAIL09.um.umsystem.edu}
9, 23 -- X-MS-Has-Attach:
9, 23 -- X-MS-TNEF-Correlator:
9, 23 -- Thread-Topic: Digital Imaging in the SEM
9, 23 -- thread-index: AcZdb9NJLUmpp7FfTHuo9NxbGK2YVQ==
9, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
9, 23 -- To: {microscopy-at-microscopy.com}
9, 23 -- X-OriginalArrivalTime: 11 Apr 2006 13:57:10.0871 (UTC) FILETIME=[D426E670:01C65D6F]
9, 23 -- Content-Transfer-Encoding: 8bit
9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3BDvB7P019836
==============================End of - Headers==============================




From: Geoffrey_Williams-at-brown.edu
Date: Tue, 11 Apr 2006 10:19:31 -0500
Subject: [Microscopy] Digital Imaging with PMTs (was SEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I morphed the subject to encompass all PMT collection systems, as that is really where my issue with 8 bit comes from. And it is not so much a problem with 8 bit.

That I think should be clarified.

It isn't so much a problem with the number of levels, but where the levels fall on the histogram and what the users 'attempt' to do with the levels once they collect the image.

Steve's grenade is a much appreciated catalyst. It isn't just SEM images that are the subject of over processing with Photoshop, Confocal images fit the same problem. And the systems compound the issue by pseudo coloring a black and white signal. At least in the SEM we can more intuitively relate to a black and white image (but why people would ever want to false color them for scientific purposes beyond combining various backscatter signals with a secondary signal is beyond me). Confocal users take the same PMT collection system, often with significantly fewer 'true' levels than 8-bit. So why the problem with 8 bit? Its much more challenging to get good distriminatory signal from a poor 8-bit image than a 12 or 16-bit image. 12 and 16 are exceedingly excessive bit-depths for nearly all output devices, especially for powerpoint display, so why use them?

Because students, researchers, and users don't have time, patience or the resources to learn how to do as John Delly instructed Frank to collect micrographs.

It is NOT challenging to open the awareness of the image. And it is the fundamental focus of my instruction on the SEM once we get past how to turn the beam on and align the system and focus. It absolutely is the Art in Science. And that is where 8-bit digital (not your ADC in the SEM) fails the general user population.

The faster way to correct the problem of poor images, and I don't need to tell this list that the good images are under-represented in the literature now, is increase digital bit depth storage and handling of all but the final version of the image. Coupled with a user who does not under- or over-saturate any part of the image, that will go a very long way to starting the microscopy and scientific community on the road back to aesthetically acceptable images.

The best impression "phrase" I use is to tell the people I am training, that their images reflect directly on them, their PI, and the university. If you strive to create beautiful images that have support their research the response from seminar attendees or poster session visitors will be much more positive than with a poor image. High quality, aesthetically pleasing, images will advance your career faster than poor images.

I feel we may move on (as Karl concludes) but, as long as the human eye is used for communication the demand for quality images will remain. It is only 'our' collective failing of our 'clients' that helps to accelerate the challenges in imaging.

The critical element of the discussion should not be that the darkroom was just as manipulative, because it wasn't. Darkrooms have one significant critical difference. You still (Polaroid excepted and that isn't darkroom) have an original negative to compare the information presented in the print. Also Randy's inclusion of music to the analogy begs to bring in MP3 sampling. At what sampling interval can you hear the digitization? That is a personal matter. Some people just want a bazillion songs, some want absolute clarity on the songs they have, then the speaker question little tiny ones or great big ones. All these variables make it easier a bit for many people to think in terms of photographic quality and what not.

(did I just detonate the grenade or am I just writing on borrowed time?)

Geoff Williams
Leduc Bioimaging Facility Manager
Brown University
 
http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/



==============================Original Headers==============================
14, 29 -- From Geoffrey_Williams-at-brown.edu Tue Apr 11 10:19:25 2006
14, 29 -- Received: from pyxis.services.brown.edu (pyxis.services.brown.edu [128.148.106.154])
14, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3BFJPft030567
14, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 10:19:25 -0500
14, 29 -- Received: from ex-gateway1-out.AD.Brown.Edu (ex-gateway1.ad.brown.edu [128.148.21.51])
14, 29 -- by pyxis.services.brown.edu (Switch-3.1.8/Switch-3.1.7/) with ESMTP id k3BFJHSg000256
14, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 11:19:23 -0400 (EDT)
14, 29 -- Received: from mail1.AD.Brown.Edu ([128.148.21.31]) by ex-gateway1-out.AD.Brown.Edu with Microsoft SMTPSVC(6.0.3790.1830);
14, 29 -- Tue, 11 Apr 2006 11:19:21 -0400
14, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0
14, 29 -- Content-class: urn:content-classes:message
14, 29 -- MIME-Version: 1.0
14, 29 -- Content-Type: text/plain;
14, 29 -- charset="iso-8859-1"
14, 29 -- Subject: Digital Imaging with PMTs (was SEM)
14, 29 -- Date: Tue, 11 Apr 2006 11:19:20 -0400
14, 29 -- Message-ID: {A1A84D541C161C4C988B4E0FB38F5A0F0433382D-at-MAIL1.AD.Brown.Edu}
14, 29 -- X-MS-Has-Attach:
14, 29 -- X-MS-TNEF-Correlator:
14, 29 -- Thread-Topic: Digital Imaging with PMTs (was SEM)
14, 29 -- Thread-Index: AcZde06Ll1Wxf21WQLSJoay+yf2Dhg==
14, 29 -- From: "Williams, Geoffrey" {Geoffrey_Williams-at-brown.edu}
14, 29 -- To: {Microscopy-at-microscopy.com}
14, 29 -- X-OriginalArrivalTime: 11 Apr 2006 15:19:21.0151 (UTC) FILETIME=[4ED3ECF0:01C65D7B]
14, 29 -- X-Brown-Proofpoint: Not Infected
14, 29 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 mlx=-1 adultscore=0 adjust=0 reason=safe engine=3.1.0-06040600 definitions=3.0.0-06041103
14, 29 -- X-Brown-MailScanner-SpamCheck: not spam, rule=notspam policy=default score=0 mlx=-1 adultscore=0 adjust=0 reason=safe engine=3.1.0-06040600 definitions=3.0.0-06041103
14, 29 -- Content-Transfer-Encoding: 8bit
14, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3BFJPft030567
==============================End of - Headers==============================




From: TindallR-at-missouri.edu
Date: Tue, 11 Apr 2006 10:23:59 -0500
Subject: [Microscopy] Digital Imaging with PMTs (was SEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Ha! I was listening to vinyl.

Now I've done it....

Randy

-----Original Message-----
X-from: Geoffrey_Williams-at-brown.edu [mailto:Geoffrey_Williams-at-brown.edu]
Sent: Tuesday, April 11, 2006 10:21 AM
To: Tindall, Randy D.

I morphed the subject to encompass all PMT collection systems, as that is really where my issue with 8 bit comes from. And it is not so much a problem with 8 bit.

That I think should be clarified.

It isn't so much a problem with the number of levels, but where the levels fall on the histogram and what the users 'attempt' to do with the levels once they collect the image.

Steve's grenade is a much appreciated catalyst. It isn't just SEM images that are the subject of over processing with Photoshop, Confocal images fit the same problem. And the systems compound the issue by pseudo coloring a black and white signal. At least in the SEM we can more intuitively relate to a black and white image (but why people would ever want to false color them for scientific purposes beyond combining various backscatter signals with a secondary signal is beyond me). Confocal users take the same PMT collection system, often with significantly fewer 'true' levels than 8-bit. So why the problem with 8 bit? Its much more challenging to get good distriminatory signal from a poor 8-bit image than a 12 or 16-bit image. 12 and 16 are exceedingly excessive bit-depths for nearly all output devices, especially for powerpoint display, so why use them?

Because students, researchers, and users don't have time, patience or the resources to learn how to do as John Delly instructed Frank to collect micrographs.

It is NOT challenging to open the awareness of the image. And it is the fundamental focus of my instruction on the SEM once we get past how to turn the beam on and align the system and focus. It absolutely is the Art in Science. And that is where 8-bit digital (not your ADC in the SEM) fails the general user population.

The faster way to correct the problem of poor images, and I don't need to tell this list that the good images are under-represented in the literature now, is increase digital bit depth storage and handling of all but the final version of the image. Coupled with a user who does not under- or over-saturate any part of the image, that will go a very long way to starting the microscopy and scientific community on the road back to aesthetically acceptable images.

The best impression "phrase" I use is to tell the people I am training, that their images reflect directly on them, their PI, and the university. If you strive to create beautiful images that have support their research the response from seminar attendees or poster session visitors will be much more positive than with a poor image. High quality, aesthetically pleasing, images will advance your career faster than poor images.

I feel we may move on (as Karl concludes) but, as long as the human eye is used for communication the demand for quality images will remain. It is only 'our' collective failing of our 'clients' that helps to accelerate the challenges in imaging.

The critical element of the discussion should not be that the darkroom was just as manipulative, because it wasn't. Darkrooms have one significant critical difference. You still (Polaroid excepted and that isn't darkroom) have an original negative to compare the information presented in the print. Also Randy's inclusion of music to the analogy begs to bring in MP3 sampling. At what sampling interval can you hear the digitization? That is a personal matter. Some people just want a bazillion songs, some want absolute clarity on the songs they have, then the speaker question little tiny ones or great big ones. All these variables make it easier a bit for many people to think in terms of photographic quality and what not.

(did I just detonate the grenade or am I just writing on borrowed time?)

Geoff Williams
Leduc Bioimaging Facility Manager
Brown University
 
http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/



==============================Original Headers==============================
14, 29 -- From Geoffrey_Williams-at-brown.edu Tue Apr 11 10:19:25 2006 14, 29 -- Received: from pyxis.services.brown.edu (pyxis.services.brown.edu [128.148.106.154])
14, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3BFJPft030567
14, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 10:19:25 -0500
14, 29 -- Received: from ex-gateway1-out.AD.Brown.Edu (ex-gateway1.ad.brown.edu [128.148.21.51])
14, 29 -- by pyxis.services.brown.edu (Switch-3.1.8/Switch-3.1.7/) with ESMTP id k3BFJHSg000256
14, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 11:19:23 -0400 (EDT)
14, 29 -- Received: from mail1.AD.Brown.Edu ([128.148.21.31]) by ex-gateway1-out.AD.Brown.Edu with Microsoft SMTPSVC(6.0.3790.1830);
14, 29 -- Tue, 11 Apr 2006 11:19:21 -0400
14, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 14, 29 -- Content-class: urn:content-classes:message 14, 29 -- MIME-Version: 1.0 14, 29 -- Content-Type: text/plain;
14, 29 -- charset="iso-8859-1"
14, 29 -- Subject: Digital Imaging with PMTs (was SEM) 14, 29 -- Date: Tue, 11 Apr 2006 11:19:20 -0400 14, 29 -- Message-ID: {A1A84D541C161C4C988B4E0FB38F5A0F0433382D-at-MAIL1.AD.Brown.Edu}
14, 29 -- X-MS-Has-Attach:
14, 29 -- X-MS-TNEF-Correlator:
14, 29 -- Thread-Topic: Digital Imaging with PMTs (was SEM) 14, 29 -- Thread-Index: AcZde06Ll1Wxf21WQLSJoay+yf2Dhg== 14, 29 -- From: "Williams, Geoffrey" {Geoffrey_Williams-at-brown.edu} 14, 29 -- To: {Microscopy-at-microscopy.com} 14, 29 -- X-OriginalArrivalTime: 11 Apr 2006 15:19:21.0151 (UTC) FILETIME=[4ED3ECF0:01C65D7B] 14, 29 -- X-Brown-Proofpoint: Not Infected 14, 29 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 mlx=-1 adultscore=0 adjust=0 reason=safe engine=3.1.0-06040600 definitions=3.0.0-06041103 14, 29 -- X-Brown-MailScanner-SpamCheck: not spam, rule=notspam policy=default score=0 mlx=-1 adultscore=0 adjust=0 reason=safe engine=3.1.0-06040600 definitions=3.0.0-06041103 14, 29 -- Content-Transfer-Encoding: 8bit 14, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3BFJPft030567 ==============================End of - Headers==============================


==============================Original Headers==============================
23, 24 -- From TindallR-at-missouri.edu Tue Apr 11 10:23:59 2006
23, 24 -- Received: from um-exproto8.um.umsystem.edu (um-exproto8.um.umsystem.edu [207.160.151.48])
23, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3BFNwh2005529
23, 24 -- for {microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 10:23:58 -0500
23, 24 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto8.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830);
23, 24 -- Tue, 11 Apr 2006 10:23:57 -0500
23, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
23, 24 -- Content-class: urn:content-classes:message
23, 24 -- MIME-Version: 1.0
23, 24 -- Content-Type: text/plain;
23, 24 -- charset="iso-8859-1"
23, 24 -- Subject: RE: [Microscopy] Digital Imaging with PMTs (was SEM)
23, 24 -- Date: Tue, 11 Apr 2006 10:23:56 -0500
23, 24 -- Message-ID: {BA876152E8653240BE8572E897083EE701849ED8-at-UM-EMAIL09.um.umsystem.edu}
23, 24 -- X-MS-Has-Attach:
23, 24 -- X-MS-TNEF-Correlator:
23, 24 -- Thread-Topic: [Microscopy] Digital Imaging with PMTs (was SEM)
23, 24 -- thread-index: AcZde5MhM12uce+QT36gAfhYjFSEyAAAEq/A
23, 24 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
23, 24 -- To: {Geoffrey_Williams-at-brown.edu}
23, 24 -- Cc: {microscopy-at-microscopy.com}
23, 24 -- X-OriginalArrivalTime: 11 Apr 2006 15:23:57.0689 (UTC) FILETIME=[F3A84690:01C65D7B]
23, 24 -- Content-Transfer-Encoding: 8bit
23, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3BFNwh2005529
==============================End of - Headers==============================




From: tonygr-at-MIT.EDU
Date: Tue, 11 Apr 2006 10:38:40 -0500
Subject: [Microscopy] Re: Digital Imaging in the SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Surely the simple act of selecting a contrast grade of printing paper
is "manipulating" the image????


Tony

At 10:02 AM 4/11/2006, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA

Tel: 617-253-4622
Fax: 617-258-6478
************************************************


==============================Original Headers==============================
10, 27 -- From tonygr-at-MIT.EDU Tue Apr 11 10:38:40 2006
10, 27 -- Received: from biscayne-one-station.mit.edu (BISCAYNE-ONE-STATION.MIT.EDU [18.7.7.80])
10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3BFcdjE017525
10, 27 -- for {microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 10:38:39 -0500
10, 27 -- Received: from outgoing.mit.edu (OUTGOING-AUTH.MIT.EDU [18.7.22.103])
10, 27 -- by biscayne-one-station.mit.edu (8.13.6/8.9.2) with ESMTP id k3BFYVSC012336
10, 27 -- for {microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 11:34:31 -0400 (EDT)
10, 27 -- Received: from emlab.mit.edu (EMLAB.MIT.EDU [18.82.0.121])
10, 27 -- (authenticated bits=0)
10, 27 -- (User authenticated as tonygr-at-ATHENA.MIT.EDU)
10, 27 -- by outgoing.mit.edu (8.13.6/8.12.4) with ESMTP id k3BFYSAg015475
10, 27 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT)
10, 27 -- for {microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 11:34:29 -0400 (EDT)
10, 27 -- Message-Id: {6.2.3.4.2.20060411113414.01e150e0-at-po9.mit.edu}
10, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4
10, 27 -- Date: Tue, 11 Apr 2006 11:35:18 -0400
10, 27 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com}
10, 27 -- From: Anthony Garratt-Reed {tonygr-at-MIT.EDU}
10, 27 -- Subject: Re: [Microscopy] Digital Imaging in the SEM
10, 27 -- In-Reply-To: {200604111402.k3BE2sO9028749-at-ns.microscopy.com}
10, 27 -- References: {200604111402.k3BE2sO9028749-at-ns.microscopy.com}
10, 27 -- Mime-Version: 1.0
10, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
10, 27 -- X-Spam-Score: 1.217
10, 27 -- X-Spam-Level: * (1.217)
10, 27 -- X-Spam-Flag: NO
10, 27 -- X-Scanned-By: MIMEDefang 2.42
==============================End of - Headers==============================




From: Mike.Bode-at-olympus-sis.com
Date: Tue, 11 Apr 2006 11:09:43 -0500
Subject: [Microscopy] Ethics & Digital Imaging (long)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Hello Michael,

There are other organizations that deal with this issue. Most of them (at least as far as I know) come to the same conclusions: The original image must be stored somewhere and must not be altered. What an original image is, however, is open to discussion. For example, a camera on a microscope may do some sharpening inside the camera. Sometimes that is not known to the users. On an SEM, you have options to influence the signal before it is shown on the screen or written to Hard disk (mix SE and BSE, for example). The consensus seems to be, that you need to store the image in that form that you first acquired and that you selected to make any sort of analysis of. In the case of an SEM this should be the image as you see it on the screen, for a camera likewise. Any processing that you do afterwards needs to be reported, but the exact details depend on who you are doing this for. I believe the forensics community has very strict guidelines regarding gamma and/or brightness, other communities may not.

How you actually achieve the above goal is probably not something a committee can make any suggestions about. Whether you store it in a read-only directory, make safety backups, or write it to CD-R directly depends on the technology you use, and any committee should not define which technology you use.

The same probably applies to making suggestions about writing the processing into a file or to the TIF header. I agree that it would be useful to have a standard way to do this, but there is no common language that can be used, nor are there even common names for the processes. One person calls a shading correction what another person calls unsharp masking. Some people use Basic as a language, others use C or Java. By defining a preference of one over the other, you would probably lose some of the capabilities that are available in different implementations. I don't think that that can be the goal of a committee. Incidentally, all our software products DO have the capability to record a history of all processing done to the images. You can even re-run the processing or apply it to another image. This is NOT a functionality that is limited to Photoshop. In addition, our software has many, many "import filters" for different SEMs, and we can probably read most of the formats out there and interpret the data correctly. If you put the images into a database that comes with the software, you can setup fields for the different pieces of information and get some safety that way. But rest assured, there is always a way someone can destroy data.

The TIF standard is very flexible, which may be the source of some of the problems, and can be confusing. For example, there is a public TIFF tag for "Resolution". However, if you use that and then try to print the image in, say, Microsoft Word, it will be interpreted literally. I.e., Word will try to print the image at the resolution that is stored there. In other words, you will see a tiny dot on the paper. That means, that applications have to find another way of storing that information, and for that they have to use "private tags" which can only be interpreted if the coding is known. I think, that a standardization of more TIF tags might help, but this then begs the next question: Image files get bigger and bigger (for example, we now acquire whole slide images, which can be GB in size). Those images cannot be dealt with effectively through TIF and other formats are needed. If a committee starts codifying the information and technology, it might stop or slow down progress in the field. Any committee has to be very careful with that. This also applies to selecting certain technologies. I have no doubt that Photoshop is a powerful product, but can it, for example, deal with GB datasets in an efficient way? Can it deal with 3D, 4D, 6D datasets (X,Y, X, time, spectral information, Fluorescence, etc.)? If not, then selecting this technology over other, perhaps not as widely accepted ones, would be a mistake.

The only way to deal with this is to allow people and companies to develop technology and let the users and markets decide what happens. This happened with TIF also. Before TIF was widely accepted, there were many different image formats, many of them proprietary. TIF had much to offer, and most companies moved to using TIF as a standard. If someone comes up with a brilliant new format that is better and more secure, I am sure that users will put pressure on companies to use that format, and the companies will eventually have to comply.

Sorry for rambling...

Michael Bode, Ph.D.

General Manager

OLYMPUS SOFT IMAGING SOLUTIONS
12596 West Bayaud Ave #300
Lakewood, CO 80228
USA
Tel.: +1 (303) 234-9270
Fax.: +1 (303) 234-9271
E-mail: Mike.Bode-at-olympus-sis.com
www.olympus-sis.com

-----Original Message-----
X-from: michael-at-Shaffer.net [mailto:michael-at-Shaffer.net]
Sent: Monday, April 10, 2006 10:25 AM
To: Mike Bode

The other day I mistakenly destroyed relavent information regarding a SEM digital image. I instantly realized what I had done (... what a blunder!), and I should have known better. The mistake reminded me I had intended to read the article entitled "Ethics and Digital Imaging" (Microscopy Today, V14,N1, Jan06).

The article's recommendations surprised me a bit relative to what I had considered a severe blunder. That is, I had not altered the image in any way, other than to make minor adjustments to brightness, contrast and gamma (i.e., adjustments the article would lead us to believe are relatively innocent, and do not significantly alter the image's statement of evidence). I actually agree with the committee's recommendations; so, what did I do that was so wrong? I SAVED the file with the SAME filename! In doing so, I had completely wiped pertinent information in the file format that the SEM had written to the TIFF format. Such information would have been important for duplicating the image, and under what circumstances the image was acquired (e.g., keV, mag, tilt, detector, chamber pressure, etc ... in fact, a veritable wealth of information).

Like many of you, I was aware of the information, and should have known better. It is now my practice for the SEM to write to a protected directory. Relative to user retrieval, is now a read only directory. This subject I expect the MSA sub-committee will address in a future report.

Regarding the reproducibility of image presentations, I also believe it's worth mentioning several other issues that should have been addressed:

The first issue is that softwares, like Photoshop, are capable of writing the history of modifications to the file (as well to a separate text file).
Actually, I am not aware of any other software with this capability, but the possibility is an open standard for the TIFF file format. It is there for any other software to implement. It may be that the MSA sub-committee didn't want to suggest that we use a specific software, but I do believe they should have mentioned the possibility, and that it would have been that more "ethical" to use software that enabled the capability. Not to suggest this method is perfect, but it is much easier than making the same entries in your lab notes.

My last issue is a long and more complex rant, but it is connected to what makes documenting the file's history possible. It is also about the microscope specific information I mistakenly (however "innocently") obliterated. That is, I don't know of a single SEM manufacturer who takes advantage of the TIFF file format such that these accidents do not happen.
The problem (IMHO) is that SEM manufacturers take advantage of the flexibility of the TIF format, but in a way that only they know how to read.
Some SEMs will simply append the data onto the end of the file, and others will embed it in the header (examples included below). There is at least one 3rd party software that knows how to read at least one SEM TIFF format, but given the popularity, unique versatility, and availability of Photoshop (and other softwares), this data should be standardized and written to the TIFF such that it will remain safely. A case in point is the metadata, made popular by today's digital cameras, that most (if not all) TIFF softwares respect and will re-write to the TIFF file, including JPEGs. Furthermore, Photoshop does not need to know it is there. Photoshop will simply recognize the beginning and end of the XMP data, and re-write it when the file is saved If XMP fields are created and standardized, it will make it easy for microscopy softwares (and Photoshop compatible plug-ins) to find and use.

Lastly ... I have no connection with Adobe relative to recommending their products. I am sure I'm not alone in recognizing Adobe's contributions to digital photography, supported by a huge user-base and many professionals.
Adobe also comes up with good ideas and provides a open forum and a means for creating standards when no one else will. For concluding my rant in a small way, can I suggest we expand this MSA subcommittee's mandate to look into, ask this microscope community for suggestions for the types of data, and follow the likes of NASA who created standards for embedding GPS information in their image files.

genuinely :o)
michael shaffer

SEM/MLA Research Coordinator
(709) 737-6799 (ofc)
(709) 737-6790 (lab)
(709) 737-6193 (FAX)
{http://www.mun.ca/creait/maf/}
{http://www.esd.mun.ca/epma/}

Inco Innovation Centre
c/o Memorial University
230 Elizabeth Avenue
P.O. Box 4200
St. John's, NL A1C 5S7


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
FEI data (appended to end of file)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
[User]
User=XTLIB
Usertext=Quanta W
UsertextUnicode=5100750061006E007400610020005700
Date=03/31/2004
Time=01:29PM

[SYSTEM]
DNumber=D7500
Software=2.2
Source=W-Tetrode
Column=W-ESEM
FinalLens=W-ESEM
Chamber=
Stage=
Pump=TMP
ESEM=
Aperture=Fixed
Scan=dispb1.0
Acq=ViperQuad1.0
EucWD=0.01

[Beam]
HV=20000
Spot=6
StigmatorX=0.39061
StigmatorY=0.446543
BeamShiftX=0
BeamShiftY=0
ScanRotation=0
ImageMode=HR

[Scan]
InternalScan=true
Dwelltime=0.0001
FrameTime=94.251
PixelHeight=2.14827e-006
PixelWidth=2.14827e-006
Horfieldsize=0.00219983
Verfieldsize=0.00189907
Average=0
Integrate=0

[Stage]
StageX=0.00296175
StageY=0.00145804
StageZ=0.0100002
StageR=3.14107
StageT=-0.000855211
Spectilt=
WorkingDistance=0.00999534

[Vacuum]
UserMode=Highvacuum
CHPressure=90
Gas=

[Specimen]
Temperature=

[Detectors]
Number=1
Name=SSD

[SSD]
Contrast=72.9
Brightness=39
Signal=BSE
Mix=100
State=true
Active=true
Mode=0
ContrastDB=72.9
BrightnessDB=39
SegmentMode=0
Setting=A+B
ContrastSpotsizeAlignment=1
MinimumDetectorDwellTime=1e-006
[PrivateFei]
BitShift=0
Databarheight=0
DataBarSelected=HV Spot WD Sig Mag HFW MicronBar Label
TimeOfCreation=31.03.2004 13:41:17


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Zeiss data (embedded in header)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
4.157207e-006
8.409719e+001
6
2.000000e+004
2.660000e+000
2.080923e-010
8.303549e-003
1
4.157207e-006
8.409719e+001
6
2.000000e+004
2.660000e+000
2.080923e-010
8.303549e-003
2
4.157207e-006
8.409719e+001
6
2.000000e+004
2.660000e+000
2.080923e-010
8.303549e-003
3
4.157207e-006
8.409719e+001
6
2.000000e+004
2.660000e+000
2.080923e-010
8.303549e-003
36
AP_APERTURESIZE
Aperture Size = 100.0 µm
AP_BEAM_CURRENT
Beam Current = 100.0 µA
DP_RUNUPSTATE
Beam State = Beam On
AP_BEAM_TIME
Beam Time = 106.72 Hours
AP_BRIGHTNESS
Brightness = 48.3 %
AP_CHAMBER_PRESSURE
Chamber = 1.30e-003 Pa
AP_COLLECTOR_BIAS
Collector Bias = 250 V
AP_CONTRAST
Contrast = 26.6 %
AP_FRAME_TIME
Cycle Time = 40.3 Secs
AP_DATE
Date :11 Mar 2004
AP_ACTUALKV
EHT = 20.00 kV
DP_FIXED_APERTURE2
EP Aperture = None
DP_EP_MODE
EP Mode = Dry
AP_ACTUALCURRENT
Fil I = 2.660 A
AP_FILAMENT_AGE
Filament Age = 18.42 Hours
DP_FILAMENT_TYPE
Filament Type = W (Agar A054)
DP_FIXED_APERTURE
Fixed Aperture (VP) = Yes
AP_FRAME_AVERAGE_COUNT
Frames to average = 1
AP_FRAME_INT_COUNT
Frames to Int. = 0
AP_IPROBE
I Probe = 208 pA
AP_LINE_AVERAGE_COUNT
Line Avg.Count = 4
AP_LINE_INT_COUNT
Line int. count = 0
AP_MAG
Mag = 84 X
DP_OUT_DEV
Output dev = 19/21 inch display
DP_OUT_TYPE
Output To = Display/File
AP_HCSTAGE_TEMP
Peltier Temp = 20.0 °C
DP_PIXEL_SIZE
Pix Size state = calibrated
AP_PIXEL_SIZE
Pixel Size = 4.157 µm
DP_DETECTOR_CHANNEL
Signal A = SE1
DP_IMPLIED_DETECTOR
Signal B = SE1
AP_STAGE_AT_X
Stage at X = 54.327 mm
AP_STAGE_AT_Y
Stage at Y = 40.286 mm
AP_STAGE_AT_Z
Stage at Z = 15.481 mm
DP_USER
User = Busy
SV_USER_NAME
User Name = CAMKR
SV_USER_TEXT
User Text = text



==============================Original Headers==============================
24, 20 -- From Michael-at-Shaffer.net Mon Apr 10 11:22:12 2006 24, 20 -- Received: from ws6-3.us4.outblaze.com (ws6-3.us4.outblaze.com [205.158.62.199])
24, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k3AGM9d5031317
24, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 11:22:10 -0500
24, 20 -- Received: (qmail 20070 invoked from network); 10 Apr 2006 16:22:08 -0000 24, 20 -- Received: from unknown (HELO roamingwolf) (Michael-at-Shaffer.net-at-134.153.130.141)
24, 20 -- by ws6-3.us4.outblaze.com with SMTP; 10 Apr 2006 16:22:06 -0000
24, 20 -- From: "michael shaffer" {michael-at-Shaffer.net} 24, 20 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com} 24, 20 -- Subject: Ethics & Digital Imaging (long) 24, 20 -- Date: Mon, 10 Apr 2006 13:52:04 -0230 24, 20 -- Message-ID: {002001c65cba$e8d76320$b995fea9-at-roamingwolf}
24, 20 -- MIME-Version: 1.0
24, 20 -- Content-Type: text/plain;
24, 20 -- charset="iso-8859-1"
24, 20 -- X-Mailer: Microsoft Office Outlook 11 24, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 24, 20 -- Thread-Index: AcZcuuc5vNdzeoaRTj+XFLjeiFV89g== 24, 20 -- Content-Transfer-Encoding: 8bit 24, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3AGM9d5031317 ==============================End of - Headers==============================


==============================Original Headers==============================
41, 23 -- From Mike.Bode-at-olympus-sis.com Tue Apr 11 11:09:43 2006
41, 23 -- Received: from mail.soft-imaging.de (mail.soft-imaging.de [62.180.61.131])
41, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3BG9gD6027584
41, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 11:09:43 -0500
41, 23 -- Received: from muenster.soft-imaging.net (muenster.soft-imaging.de [10.26.20.9])
41, 23 -- by mail.soft-imaging.de (8.11.6/8.11.6/SuSE Linux 0.5) with ESMTP id k3BG9g423642
41, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 18:09:42 +0200
41, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
41, 23 -- Content-class: urn:content-classes:message
41, 23 -- MIME-Version: 1.0
41, 23 -- Content-Type: text/plain;
41, 23 -- charset="iso-8859-1"
41, 23 -- Subject: Re: [Microscopy] Ethics & Digital Imaging (long)
41, 23 -- Date: Tue, 11 Apr 2006 18:06:00 +0200
41, 23 -- Message-ID: {78B53BA1C5A2D9449EDA30A98800EC94174CC8-at-ms-s-gws.soft-imaging.net}
41, 23 -- X-MS-Has-Attach:
41, 23 -- X-MS-TNEF-Correlator:
41, 23 -- Thread-Topic: Re: [Microscopy] Ethics & Digital Imaging (long)
41, 23 -- Thread-Index: AcZcu11KZEmu0TY5QBm2H0F4QYS2agAA3NuwAAHWLjAALsZUoA==
41, 23 -- From: "Mike Bode" {Mike.Bode-at-olympus-sis.com}
41, 23 -- To: {Microscopy-at-microscopy.com}
41, 23 -- Content-Transfer-Encoding: 8bit
41, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3BG9gD6027584
==============================End of - Headers==============================




From: michael-at-Shaffer.net
Date: Tue, 11 Apr 2006 11:13:36 -0500
Subject: [Microscopy] RE: Digital Imaging in the SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Steve :o)

Your comments are well taken ...

In my case, I get to blame it on the lousy monitor that came with my SEM.
It is the least expensive LCD that HP makes, and its gamma ranges from 2.0
to 2.5 depending on the angle of view ... and the dark shades are worse
straight-on!!! ... In any case ... A few tonal tweaks with Photoshop make
the images much better, and PS also interfaces much better with printers
than otherwise.

genuinely :o)
michael shaffer

SEM/MLA Research Coordinator
(709) 737-6799 (ofc)
(709) 737-6790 (lab)
(709) 737-6193 (FAX)
{http://www.mun.ca/creait/maf/}
{http://www.esd.mun.ca/epma/}

Inco Innovation Centre
c/o Memorial University
230 Elizabeth Avenue
P.O. Box 4200
St. John's, NL A1C 5S7



} -----Original Message-----
} From: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com]
} Sent: April 11, 2006 8:01 AM
} To: michael-at-shaffer.net
} Subject: [Microscopy] Digital Imaging in the SEM
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} Hi All
}
} World famous for throwing in a grenade (just ask my friends)
} it never ceases to fascinate me of how popular the processing
} of SEM images seems to be. Go to a conference and it is only
} the person demonstrating Photoshop who manages to fill the
} lecture theatre!
}
} Have we forgotten how to use the SEM, use the features
} correctly and optimise the photographic/imaging contrast and
} brightness? In the days when some of my clients produced
} 15,000 Polaroid pictures per year 90% of then looked pretty
} good as they came off the machine, it seems that today 50%
} have to be "improved" by artificial means?
}
} Are we substituting talented computer operators for talented
} SEM operators, it does not seem that we are scientists in
} microscopy any more?
}
} Think on :)
}
} Steve Chapman
} Senior Consultant Protrain
} For electron microscopy consultancy and training world wide
} Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711
} 606967 Web www.emcourses.com
}
}
} ==============================Original
} Headers==============================
} 7, 29 -- From protrain-at-emcourses.com Tue Apr 11 05:30:00 2006
} 7, 29 -- Received: from smtp01.x-mailer.co.uk
} (smtp01.x-mailer.co.uk [195.13.65.37])
} 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
} with ESMTP id k3BAU0VE019274
} 7, 29 -- for {microscopy-at-microscopy.com} ; Tue, 11 Apr
} 2006 05:30:00 -0500
} 7, 29 -- Received: from [62.69.65.169] (helo=smtp-f.mail.legend.co.uk)
} 7, 29 -- by smtp01.x-mailer.co.uk with smtp (Exim 4.60)
} 7, 29 -- (envelope-from {protrain-at-emcourses.com} )
} 7, 29 -- id 1FTG8E-0006LI-VV
} 7, 29 -- for microscopy-at-microscopy.com; Tue, 11 Apr 2006
} 11:29:59 +0100
} 7, 29 -- Received: (qmail 12648 invoked from network); 11 Apr
} 2006 10:29:48 -0000 7, 29 -- Received: from unknown (HELO
} advent) (212.248.148.141)
} 7, 29 -- by 0 with SMTP; 11 Apr 2006 10:29:48 -0000
} 7, 29 -- Message-ID: {00a801c65d53$50db83f0$3493f8d4-at-advent}
} 7, 29 -- Reply-To: "Steve Chapman" {protrain-at-emcourses.com}
} 7, 29 -- From: "Steve Chapman" {protrain-at-emcourses.com} 7, 29
} -- To: "American Soc" {microscopy-at-microscopy.com} 7, 29 --
} Subject: Digital Imaging in the SEM 7, 29 -- Date: Tue, 11
} Apr 2006 10:42:37 +0100 7, 29 -- Organization: Protrain 7, 29
} -- MIME-Version: 1.0 7, 29 -- Content-Type: text/plain;
} 7, 29 -- format=flowed;
} 7, 29 -- charset="iso-8859-1";
} 7, 29 -- reply-type=original
} 7, 29 -- Content-Transfer-Encoding: 7bit 7, 29 -- X-Priority:
} 3 7, 29 -- X-MSMail-Priority: Normal 7, 29 -- X-Mailer:
} Microsoft Outlook Express 6.00.2900.2527 7, 29 -- X-MimeOLE:
} Produced By Microsoft MimeOLE V6.00.2900.2527
} ==============================End of -
} Headers==============================
}


==============================Original Headers==============================
10, 20 -- From Michael-at-Shaffer.net Tue Apr 11 11:13:36 2006
10, 20 -- Received: from ws6-1.us4.outblaze.com (ws6-1.us4.outblaze.com [205.158.62.196])
10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k3BGDZBq000655
10, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 11:13:35 -0500
10, 20 -- Received: (qmail 25930 invoked from network); 11 Apr 2006 16:13:35 -0000
10, 20 -- Received: from unknown (HELO roamingwolf) (Michael-at-Shaffer.net-at-134.153.130.141)
10, 20 -- by ws6-1.us4.outblaze.com with SMTP; 11 Apr 2006 16:13:34 -0000
10, 20 -- From: "michael shaffer" {michael-at-Shaffer.net}
10, 20 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com}
10, 20 -- Subject: RE: [Microscopy] Digital Imaging in the SEM
10, 20 -- Date: Tue, 11 Apr 2006 13:43:33 -0230
10, 20 -- Message-ID: {002901c65d82$e224ec00$37079986-at-roamingwolf}
10, 20 -- MIME-Version: 1.0
10, 20 -- Content-Type: text/plain;
10, 20 -- charset="US-ASCII"
10, 20 -- Content-Transfer-Encoding: 7bit
10, 20 -- X-Mailer: Microsoft Office Outlook 11
10, 20 -- In-Reply-To: {200604111030.k3BAUs1p020086-at-ns.microscopy.com}
10, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
10, 20 -- Thread-Index: AcZdUwOOYC6gjj9qTYCyCv805nEsBgACIEEg
==============================End of - Headers==============================




From: bfoster-at-mme1.com
Date: Tue, 11 Apr 2006 12:00:50 -0500
Subject: [Microscopy] Re: Fluorescence/SEM correlative microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Randy

Not FL + SEM, but FL + AFM, simultaneously. What do you need to image?

If FL+SEM, I think that there are finder stages that can be used for both. Contact Bill Miller at microbill-at-mohawk.net. He is likely to have the answer.

Good hunting.

B
At 03:34 PM 4/10/2006, TindallR-at-missouri.edu wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 17 -- From bfoster-at-mme1.com Tue Apr 11 12:00:50 2006
9, 17 -- Received: from 5starpro.com (enterprise.5starpro.com [207.44.136.95])
9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3BH0oL3015012
9, 17 -- for {microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 12:00:50 -0500
9, 17 -- Received: (qmail 9533 invoked by uid 2020); 11 Apr 2006 13:02:14 -0400
9, 17 -- Received: from host57-99.rancor.birch.net (HELO barbsd505.mme1.com) (65.17.57.99)
9, 17 -- by enterprise.5starpro.com with (DHE-RSA-AES256-SHA encrypted) SMTP; 11 Apr 2006 13:02:14 -0400
9, 17 -- Message-Id: {7.0.1.0.0.20060411115858.020cd2b0-at-mme1.com}
9, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0
9, 17 -- Date: Tue, 11 Apr 2006 12:00:59 -0500
9, 17 -- To: TindallR-at-missouri.edu, Microscopy Listserver {microscopy-at-microscopy.com}
9, 17 -- From: Barbara Foster {bfoster-at-mme1.com}
9, 17 -- Subject: Re: [Microscopy] Fluorescence/SEM correlative microscopy
9, 17 -- In-Reply-To: {200604102034.k3AKYvun015359-at-ns.microscopy.com}
9, 17 -- References: {200604102034.k3AKYvun015359-at-ns.microscopy.com}
9, 17 -- Mime-Version: 1.0
9, 17 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: bfoster-at-mme1.com
Date: Tue, 11 Apr 2006 12:03:33 -0500
Subject: [Microscopy] Re: Digital Imaging in the SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

... Just a curious note on this subject: Ansel Adams used a microwave for final processing of many of his images and developed quite a protocol for setting and timing... Does that constitute "manipulation"?

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text for the Spring semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.

At 09:00 AM 4/11/2006, TindallR-at-missouri.edu wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
9, 17 -- From bfoster-at-mme1.com Tue Apr 11 12:03:33 2006
9, 17 -- Received: from 5starpro.com (enterprise.5starpro.com [207.44.136.95])
9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3BH3XeF018497
9, 17 -- for {microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 12:03:33 -0500
9, 17 -- Received: (qmail 9631 invoked by uid 2020); 11 Apr 2006 13:04:57 -0400
9, 17 -- Received: from host57-99.rancor.birch.net (HELO barbsd505.mme1.com) (65.17.57.99)
9, 17 -- by enterprise.5starpro.com with (DHE-RSA-AES256-SHA encrypted) SMTP; 11 Apr 2006 13:04:57 -0400
9, 17 -- Message-Id: {7.0.1.0.0.20060411120143.020cd2b0-at-mme1.com}
9, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0
9, 17 -- Date: Tue, 11 Apr 2006 12:03:40 -0500
9, 17 -- To: TindallR-at-missouri.edu, Microscopy Listserver {microscopy-at-microscopy.com}
9, 17 -- From: Barbara Foster {bfoster-at-mme1.com}
9, 17 -- Subject: Re: [Microscopy] Digital Imaging in the SEM
9, 17 -- In-Reply-To: {200604111400.k3BE0FKN024040-at-ns.microscopy.com}
9, 17 -- References: {200604111400.k3BE0FKN024040-at-ns.microscopy.com}
9, 17 -- Mime-Version: 1.0
9, 17 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: walck-at-southbaytech.com
Date: Tue, 11 Apr 2006 12:16:45 -0500
Subject: [Microscopy] viaWWW: Tripod Polisher micrometers locking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Pedro,

This is not only a common problem with micrometers on our Tripod(TM)
Polisher and competitor units alike, but also with some of the hand
grinding and lapping units that are on the market. As you mention, the
most important thing is to keep water from getting into the parts that
are susceptible to rusting. Don't turn them upside down when wet and
dry them after you are finished. I have owned and used Tripod(TM)
polishers and both the Gatan and the Fischione hand grinding units.
Because these would see use everyday, I took them apart and used a
Lithium grease on the threads. If I remember correctly, the Lithium
grease that I used was for outboard motors and is water repellent. (You
can get this type of grease almost anywhere.) By being careful and
periodically lubricating the threads and moving parts, I never had a
unit fail on me from corrosion. In fact, I never had a unit fail at
all. If you get a tube of this grease, with the amount that you will
use, it should last about a thousand years. In other words, just use it
very sparingly. If during a session, you think that you may have gotten
water into the sliding portion of the micrometers or onto the threads,
wait until after you are done with your session and when you are
cleaning up, disassemble the units, dry them, and then reapply the thin
layer of grease.

For completeness, on the South Bay Technology hand lapping fixtures, the
parts that could be exposed to water are all stainless steel and the
sliding surfaces have a solid film lubricant on them. With these
fixtures, they can get "sticky" if water is allowed to get into the
sliding surfaces. When this occurs, simply take them apart and wipe the
sliding surfaces with a dry cloth or paper towel and put them together
again. This will remove an oxide that forms on the solid lubricant due
to the water exposure and expose good solid lubricant again. The piston
should slide easily again.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: pedromfjcosta-at-gmail.com [mailto:pedromfjcosta-at-gmail.com]
Sent: Monday, April 10, 2006 9:48 PM
To: Walck-at-SouthBayTech.com

This Question/Comment was submitted to the Microscopy Listserver using
the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both pedromfjcosta-at-gmail.com as well as the MIcroscopy
Listserver
------------------------------------------------------------------------
---

Email: pedromfjcosta-at-gmail.com
Name: Pedro MFJ Costa

Organization: University of Cambridge

Title-Subject: [Filtered] Tripod Polisher micrometers locking

Question: We have several tripod polishers in your lab and,
unfortunately, we find that with time the precision micrometers tend to
get locked up. In fact, despite trying to avoid excessive water
infiltrating the micrometers they still become rusty.

I was wondering if anyone had a way to prevent this from happening or
possibly to unlock them without incurring into further damage.


------------------------------------------------------------------------
---

==============================Original
Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Mon Apr 10 23:44:01 2006 8, 12 --
Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id k3B4i0Dm027217
8, 12 -- for {microscopy-at-microscopy.com} ; Mon, 10 Apr 2006
23:44:00 -0500
8, 12 -- Mime-Version: 1.0
8, 12 -- X-Sender: (Unverified)
8, 12 -- Message-Id: {p06110403c060e47f14e0-at-[206.69.208.22]}
8, 12 -- Date: Mon, 10 Apr 2006 23:43:57 -0500
8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: pedromfjcosta-at-gmail.com (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: Tripod Polisher micrometers locking 8, 12 --
Content-Type: text/plain; charset="us-ascii"
==============================End of -
Headers==============================


==============================Original Headers==============================
20, 27 -- From walck-at-southbaytech.com Tue Apr 11 12:16:45 2006
20, 27 -- Received: from ylpvm12.prodigy.net (ylpvm12-ext.prodigy.net [207.115.57.43])
20, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3BHGiU3002118
20, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 12:16:44 -0500
20, 27 -- Received: from pimout7-ext.prodigy.net (pimout7-int.prodigy.net [207.115.4.147])
20, 27 -- by ylpvm12.prodigy.net (8.12.10 outbound/8.12.10) with ESMTP id k3BHGZbL009994
20, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 13:16:36 -0400
20, 27 -- X-ORBL: [64.169.193.90]
20, 27 -- Received: from dynamicbl8uno3 (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90])
20, 27 -- by pimout7-ext.prodigy.net (8.13.6 out.dk/8.13.6) with ESMTP id k3BHGaNm148486;
20, 27 -- Tue, 11 Apr 2006 13:16:41 -0400
20, 27 -- From: "Scott Walck" {walck-at-southbaytech.com}
20, 27 -- To: {pedromfjcosta-at-gmail.com}
20, 27 -- Cc: {Microscopy-at-microscopy.com}
20, 27 -- Subject: RE: [Microscopy] viaWWW: Tripod Polisher micrometers locking and hand lapping tools
20, 27 -- Date: Tue, 11 Apr 2006 10:16:40 -0700
20, 27 -- Message-ID: {010b01c65d8b$b6690110$7801a8c0-at-dynamicbl8uno3}
20, 27 -- MIME-Version: 1.0
20, 27 -- Content-Type: text/plain;
20, 27 -- charset="us-ascii"
20, 27 -- Content-Transfer-Encoding: 7bit
20, 27 -- X-Priority: 3 (Normal)
20, 27 -- X-MSMail-Priority: Normal
20, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.2627
20, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
20, 27 -- In-Reply-To: {200604110448.k3B4mMKx001120-at-ns.microscopy.com}
20, 27 -- Importance: Normal
==============================End of - Headers==============================




From: togo-at-uvic.ca
Date: Tue, 11 Apr 2006 12:31:43 -0500
Subject: [Microscopy] Ansel's microwave

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

At 12:06 PM 4/11/2006 -0500, Barbara Foster wrote:
} ... Just a curious note on this subject: Ansel Adams used a microwave for
} final processing of many of his images and developed quite a protocol for
} setting and timing... Does that constitute "manipulation"?

I heard Ansel Adams talk about this at a conference in Asilomar years ago,
but he only talked about used the microwave to quickly dry test strips
prints in order to see what subtile highlight changes would be found
between the wet print and the dry one.

He didn't actually manipulate with the microwave ; {)
_____________________________________
Tom Gore, Advanced Imaging Laboratory
Department of Biology University of Victoria
Box 3020 Station CSC
Victoria BC V8W 3N5 Canada
voice 250 721 7134 fax 250 721 7120
web: http://web.uvic.ca/ail/


==============================Original Headers==============================
4, 20 -- From togo-at-uvic.ca Tue Apr 11 12:31:43 2006
4, 20 -- Received: from castle.comp.uvic.ca (castle.comp.uvic.ca [142.104.5.97])
4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3BHVgp9012056
4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 12:31:43 -0500
4, 20 -- Received: from amidol (amidol.biol.uvic.ca [142.104.208.15])
4, 20 -- by castle.comp.uvic.ca (8.13.6/8.13.6) with ESMTP id k3BHVeLN3645616
4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 10:31:40 -0700
4, 20 -- Message-Id: {4.2.0.58.20060411102951.052980f0-at-pop.uvic.ca}
4, 20 -- X-Sender: togo-at-pop.uvic.ca
4, 20 -- X-Mailer: QUALCOMM Windows Eudora Pro Version 4.2.0.58
4, 20 -- Date: Tue, 11 Apr 2006 10:38:11 -0800
4, 20 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com}
4, 20 -- From: Tom Gore {togo-at-uvic.ca}
4, 20 -- Subject: Ansel's microwave
4, 20 -- In-Reply-To: {200604111706.k3BH6uKL029865-at-ns.microscopy.com}
4, 20 -- Mime-Version: 1.0
4, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
4, 20 -- X-UVic-Virus-Scanned: OK - Passed virus scan by Sophos (sophie) on castle
4, 20 -- X-UVic-Spam-Scan: castle.comp.uvic.ca Not_scanned_LOCAL
4, 20 -- X-Scanned-By: MIMEDefang 2.33 (www . roaringpenguin . com / mimedefang)
==============================End of - Headers==============================




From: tivol-at-caltech.edu
Date: Tue, 11 Apr 2006 12:58:44 -0500
Subject: [Microscopy] Re: Digital Imaging in the SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Barbara,

Interesting. What was the microwave used for? Heating the developer?

For any artistic images there is of course no protocol, and none would
make sense, so for purely artistic images you can do whatever you want
to.

For scientific images there is of course a higher standard. The basic
idea of science is that experiments can be verified. That requires
complete disclosure how data were obtained. For an image it means that
anybody who is somewhat knowledgeable in the technique must be able to
recreate all steps that lead to a certain conclusion. Since much of
image processing is a destructive process (information gets thrown away
and cannot be recovered), the only way to assure that is to keep a copy
of the "original" image and then describe what was done to the image, so
someone else can take the same image, apply the same steps and come to
the same conclusion.


Michael Bode, Ph.D.

General Manager

OLYMPUS SOFT IMAGING SOLUTIONS
12596 West Bayaud Ave #300
Lakewood, CO 80228
USA
Tel.: +1 (303) 234-9270
Fax.: +1 (303) 234-9271
E-mail: Mike.Bode-at-olympus-sis.com
www.olympus-sis.com

-----Original Message-----
X-from: bfoster-at-mme1.com [mailto:bfoster-at-mme1.com]
Sent: Tuesday, April 11, 2006 11:06 AM
To: Mike Bode

... Just a curious note on this subject: Ansel Adams used a microwave
for final processing of many of his images and developed quite a
protocol for setting and timing... Does that constitute "manipulation"?

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text for the Spring
semester? Call us today to learn more about "Optimizing LIght
Microscopy". Copies still available through MME... even for class-room
lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.

At 09:00 AM 4/11/2006, TindallR-at-missouri.edu wrote:



} -----------------------------------------------------------------------
} ----- The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver

} soothing tones of Metallica playing in the background. But, I feel I
} have better control now, with less waste and time, with often superior
} results. If I ever get back into the darkroom, it will be as a hobby.
}
} Cheers,
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
} ==============================Original
} Headers==============================
} 9, 23 -- From TindallR-at-missouri.edu Tue Apr 11 08:57:12 2006 9, 23 --
} Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu
[207.160.151.49])
} 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id k3BDvB7P019836
} 9, 23 -- for {microscopy-at-microscopy.com} ; Tue, 11 Apr 2006
08:57:12 -0500
} 9, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31])
by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830);
} 9, 23 -- Tue, 11 Apr 2006 08:57:10 -0500
} 9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 23 --
} Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0
} 9, 23 -- Content-Type: text/plain;
} 9, 23 -- charset="us-ascii"
} 9, 23 -- Subject: Digital Imaging in the SEM 9, 23 -- Date: Tue, 11 Apr

} 2006 08:57:09 -0500 9, 23 -- Message-ID:
} {BA876152E8653240BE8572E897083EE701849ED4-at-UM-EMAIL09.um.umsystem.edu}
} 9, 23 -- X-MS-Has-Attach:
} 9, 23 -- X-MS-TNEF-Correlator:
} 9, 23 -- Thread-Topic: Digital Imaging in the SEM 9, 23 --
} thread-index: AcZdb9NJLUmpp7FfTHuo9NxbGK2YVQ== 9, 23 -- From: "Tindall,

} Randy D." {TindallR-at-missouri.edu} 9, 23 -- To:
} {microscopy-at-microscopy.com} 9, 23 -- X-OriginalArrivalTime: 11 Apr 2006

} 13:57:10.0871 (UTC) FILETIME=[D426E670:01C65D6F] 9, 23 --
} Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from
} quoted-printable to 8bit by ns.microscopy.com id k3BDvB7P019836
} ==============================End of -
} Headers==============================


==============================Original
Headers==============================
9, 17 -- From bfoster-at-mme1.com Tue Apr 11 12:03:33 2006 9, 17 --
Received: from 5starpro.com (enterprise.5starpro.com [207.44.136.95])
9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id k3BH3XeF018497
9, 17 -- for {microscopy-at-microscopy.com} ; Tue, 11 Apr 2006
12:03:33 -0500
9, 17 -- Received: (qmail 9631 invoked by uid 2020); 11 Apr 2006
13:04:57 -0400 9, 17 -- Received: from host57-99.rancor.birch.net (HELO
barbsd505.mme1.com) (65.17.57.99)
9, 17 -- by enterprise.5starpro.com with (DHE-RSA-AES256-SHA
encrypted) SMTP; 11 Apr 2006 13:04:57 -0400
9, 17 -- Message-Id: {7.0.1.0.0.20060411120143.020cd2b0-at-mme1.com}
9, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 9, 17 --


On Apr 11, 2006, at 10:33 AM, Mike.Bode-at-olympus-sis.com wrote:

} Interesting. What was the microwave used for? Heating the developer?
}
Dear Mike,
After the prints were exposed and developed (and, I seem to remember,
fixed) they were microwaved for a time to enhance the contrast. The
process produced very sharp, good-looking prints. Ansel Adams was
known for the amount of time, care, and use of many procedures to make
what he considered the best-looking prints from his negatives.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original Headers==============================
4, 22 -- From tivol-at-caltech.edu Tue Apr 11 12:58:44 2006
4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3BHwh7X031687
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 11 Apr 2006 12:58:43 -0500
4, 22 -- Received: from localhost (earth-dog [192.168.1.3])
4, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 6606634B28
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 11 Apr 2006 10:58:43 -0700 (PDT)
4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21])
4, 22 -- by earth-ox.its.caltech.edu (Postfix) with ESMTP id 0B06D10AD59
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 11 Apr 2006 10:54:57 -0700 (PDT)
4, 22 -- Mime-Version: 1.0 (Apple Message framework v623)
4, 22 -- In-Reply-To: {200604111733.k3BHXWwO014846-at-ns.microscopy.com}
4, 22 -- References: {200604111733.k3BHXWwO014846-at-ns.microscopy.com}
4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
4, 22 -- Message-Id: {7effee60d68b87a824bbd09d1d080e5e-at-caltech.edu}
4, 22 -- Content-Transfer-Encoding: 7bit
4, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
4, 22 -- Subject: Re: [Microscopy] Digital Imaging in the SEM
4, 22 -- Date: Tue, 11 Apr 2006 11:04:37 -0700
4, 22 -- To: microscopy-at-msa.microscopy.com
4, 22 -- X-Mailer: Apple Mail (2.623)
4, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.3.3
==============================End of - Headers==============================




From: Mike.Bode-at-olympus-sis.com
Date: Tue, 11 Apr 2006 13:11:45 -0500
Subject: [Microscopy] Re: Digital Imaging in the SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

...and beautiful pictures they are. I have a couple of reprints in my
house. I didn't know that Adams was playing with microwaves...

Michael Bode

General Manager

OLYMPUS SOFT IMAGING SOLUTIONS CORP.
12596 West Bayaud Ave #300
Lakewood, CO 80228
USA
Tel.: +1 (303) 234-9270
Fax.: +1 (303) 234-9271
E-Mail: Mike.Bode-at-olympus-sis.net
www.olympus-sis.net

-----Original Message-----
X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu]
Sent: Tuesday, April 11, 2006 11:01 AM
To: Mike Bode


On Apr 11, 2006, at 10:33 AM, Mike.Bode-at-olympus-sis.com wrote:

} Interesting. What was the microwave used for? Heating the developer?
}
Dear Mike,
After the prints were exposed and developed (and, I seem to
remember,
fixed) they were microwaved for a time to enhance the contrast. The
process produced very sharp, good-looking prints. Ansel Adams was
known for the amount of time, care, and use of many procedures to make
what he considered the best-looking prints from his negatives.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


==============================Original
Headers==============================
4, 22 -- From tivol-at-caltech.edu Tue Apr 11 12:58:44 2006
4, 22 -- Received: from outgoing-mail.its.caltech.edu
(outgoing-mail.its.caltech.edu [131.215.239.19])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id k3BHwh7X031687
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 11 Apr 2006
12:58:43 -0500
4, 22 -- Received: from localhost (earth-dog [192.168.1.3])
4, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 6606634B28
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 11 Apr 2006
10:58:43 -0700 (PDT)
4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu
[131.215.2.21])
4, 22 -- by earth-ox.its.caltech.edu (Postfix) with ESMTP id
0B06D10AD59
4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 11 Apr 2006
10:54:57 -0700 (PDT)
4, 22 -- Mime-Version: 1.0 (Apple Message framework v623)
4, 22 -- In-Reply-To: {200604111733.k3BHXWwO014846-at-ns.microscopy.com}
4, 22 -- References: {200604111733.k3BHXWwO014846-at-ns.microscopy.com}
4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
4, 22 -- Message-Id: {7effee60d68b87a824bbd09d1d080e5e-at-caltech.edu}
4, 22 -- Content-Transfer-Encoding: 7bit
4, 22 -- From: Bill Tivol {tivol-at-caltech.edu}
4, 22 -- Subject: Re: [Microscopy] Digital Imaging in the SEM
4, 22 -- Date: Tue, 11 Apr 2006 11:04:37 -0700
4, 22 -- To: microscopy-at-msa.microscopy.com
4, 22 -- X-Mailer: Apple Mail (2.623)
4, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.3.3
==============================End of -
Headers==============================


==============================Original Headers==============================
12, 24 -- From Mike.Bode-at-olympus-sis.com Tue Apr 11 13:11:44 2006
12, 24 -- Received: from mail.soft-imaging.de (mail.soft-imaging.de [62.180.61.131])
12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3BIBiIt009063
12, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Apr 2006 13:11:44 -0500
12, 24 -- Received: from muenster.soft-imaging.net (muenster.soft-imaging.de [10.26.20.9])
12, 24 -- by mail.soft-imaging.de (8.11.6/8.11.6/SuSE Linux 0.5) with ESMTP id k3BIBi427099;
12, 24 -- Tue, 11 Apr 2006 20:11:44 +0200
12, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5
12, 24 -- Content-class: urn:content-classes:message
12, 24 -- MIME-Version: 1.0
12, 24 -- Content-Type: text/plain;
12, 24 -- charset="us-ascii"
12, 24 -- Subject: RE: [Microscopy] Re: Digital Imaging in the SEM
12, 24 -- Date: Tue, 11 Apr 2006 20:07:45 +0200
12, 24 -- Message-ID: {78B53BA1C5A2D9449EDA30A98800EC94174D00-at-ms-s-gws.soft-imaging.net}
12, 24 -- X-MS-Has-Attach:
12, 24 -- X-MS-TNEF-Correlator:
12, 24 -- Thread-Topic: [Microscopy] Re: Digital Imaging in the SEM
12, 24 -- Thread-Index: AcZdkfW6jQ7Z6VoWQAyKDyjGDSEXvgAALV2A
12, 24 -- From: "Mike Bode" {Mike.Bode-at-olympus-sis.com}
12, 24 -- To: {Microscopy-at-microscopy.com}
12, 24 -- Cc: {tivol-at-caltech.edu}
12, 24 -- Content-Transfer-Encoding: 8bit
12, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3BIBiIt009063
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Wed, 12 Apr 2006 13:11:08 -0500
Subject: [Microscopy] viaWWW: Tripod Polisher micrometers locking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Pedro,
Another possibility from the boating world is to use lanolin. Some consider
it to be the best rust preventive available (used on tools kept on board).
The one drawback I can see for micrometers is that the viscosity is very
high and could cause some problems with the tight clearances on a good
micrometer. Some experimentation would be in order. Otherwise, as
mentioned, lithium grease is considered to be very waterproof and has a low
viscosity.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: pedromfjcosta-at-gmail.com [mailto:pedromfjcosta-at-gmail.com]
Sent: Tuesday, April 11, 2006 12:48 AM
To: kenconverse-at-qualityimages.biz

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying

please copy both pedromfjcosta-at-gmail.com as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: pedromfjcosta-at-gmail.com
Name: Pedro MFJ Costa

Organization: University of Cambridge

Title-Subject: [Filtered] Tripod Polisher micrometers locking

Question: We have several tripod polishers in your lab and, unfortunately,
we find that with time the precision micrometers tend to get locked up. In
fact, despite trying to avoid excessive water infiltrating the micrometers
they still become rusty.

I was wondering if anyone had a way to prevent this from happening or
possibly to unlock them without incurring into further damage.


---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Mon Apr 10 23:44:01 2006
8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
k3B4i0Dm027217
8, 12 -- for {microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 23:44:00
-0500
8, 12 -- Mime-Version: 1.0
8, 12 -- X-Sender: (Unverified)
8, 12 -- Message-Id: {p06110403c060e47f14e0-at-[206.69.208.22]}
8, 12 -- Date: Mon, 10 Apr 2006 23:43:57 -0500
8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: pedromfjcosta-at-gmail.com (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: Tripod Polisher micrometers locking
8, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================





___________________________________________________________
$0 Web Hosting with up to 200MB web space, 1000 MB Transfer
10 Personalized POP and Web E-mail Accounts, and much more.
Signup at www.doteasy.com



==============================Original Headers==============================
25, 24 -- From kenconverse-at-qualityimages.biz Wed Apr 12 13:11:07 2006
25, 24 -- Received: from qualityimages.biz (dpmail16.doteasy.com [65.61.209.16])
25, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3CIB7ee022039
25, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 12 Apr 2006 13:11:07 -0500
25, 24 -- Received: from Ken [68.64.242.101] by qualityimages.biz with ESMTP
25, 24 -- (SMTPD32-8.05) id A2BD47030146; Wed, 12 Apr 2006 11:11:09 -0700
25, 24 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
25, 24 -- To: {pedromfjcosta-at-gmail.com} ,
25, 24 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com}
25, 24 -- Subject: RE: [Microscopy] viaWWW: Tripod Polisher micrometers locking
25, 24 -- Date: Wed, 12 Apr 2006 14:11:22 -0400
25, 24 -- Message-ID: {001401c65e5c$83c4e900$6501a8c0-at-Ken}
25, 24 -- MIME-Version: 1.0
25, 24 -- Content-Type: text/plain;
25, 24 -- charset="us-ascii"
25, 24 -- X-Priority: 3 (Normal)
25, 24 -- X-MSMail-Priority: Normal
25, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.6626
25, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180
25, 24 -- Importance: Normal
25, 24 -- In-Reply-To: {200604110447.k3B4lZhZ032373-at-ns.microscopy.com}
25, 24 -- X-IMSTrailer: __IMail_7__
25, 24 -- Content-Transfer-Encoding: 8bit
25, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3CIB7ee022039
==============================End of - Headers==============================




From: dale_batchelor-at-ncsu.edu
Date: Wed, 12 Apr 2006 17:11:14 -0500
Subject: [Microscopy] AFM Short Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

} AFM short course June 12 -16, 2006

} Avoid the rush and register now!
}
} "AFM and Other Scanned Probe Microscopies" presented at N.C. State
} University in Raleigh, NC by Prof. Phil Russell, Dr. Joe Griffith,
} Alexei Gruverman and others.
} Lab sessions will utilize instrumentation from most major
} instrumentation vendors.
}
} This one-week short course has evolved from the numerous Scanned Probe
} Microscopy courses developed and taught by Prof. Russell over the past
} 2 decades. It is designed for technicians, scientists, engineers, and
} researchers. The course includes laboratories with hands-on time using
} a variety of scanning probe microscope (SPM) systems. Each student
} will receive a notebook of all materials covered in the lectures and
} animation/simulation software covering AFM principles.

} For more information go to www.ncsu.edu/aif/afmcourse

Dale Batchelor
email: dale_batchelor-at-ncsu.edu
phone: 919-515-3841


==============================Original Headers==============================
5, 19 -- From dale_batchelor-at-ncsu.edu Wed Apr 12 17:11:14 2006
5, 19 -- Received: from uni05mr.unity.ncsu.edu (uni05mr.unity.ncsu.edu [152.1.224.164])
5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3CMBEHF002904
5, 19 -- for {Microscopy-at-MSA.Microscopy.com} ; Wed, 12 Apr 2006 17:11:14 -0500
5, 19 -- Received: from [152.14.52.68] (dbmacx.aif.ncsu.edu [152.14.52.68])
5, 19 -- by uni05mr.unity.ncsu.edu (8.13.6/8.13.4/Nv5.2006.0214) with ESMTP id k3CMBATg010334
5, 19 -- for {Microscopy-at-MSA.Microscopy.com} ; Wed, 12 Apr 2006 18:11:11 -0400 (EDT)
5, 19 -- Mime-Version: 1.0 (Apple Message framework v623)
5, 19 -- Content-Transfer-Encoding: 7bit
5, 19 -- Message-Id: {be4c9456fc38738559bd47557ee6c5cc-at-ncsu.edu}
5, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed
5, 19 -- To: Microscopy-at-MSA.Microscopy.com
5, 19 -- From: Dale Batchelor {dale_batchelor-at-ncsu.edu}
5, 19 -- Subject: AFM Short Course
5, 19 -- Date: Wed, 12 Apr 2006 18:16:48 -0400
5, 19 -- X-Mailer: Apple Mail (2.623)
5, 19 -- X-PMX-Version: 5.1.2.240295, Antispam-Engine: 2.3.0.1, Antispam-Data: 2006.4.12.145109
5, 19 -- X-Spam-Status: No, Hits=7%
5, 19 -- X-Spam-Level: IIIIIII
==============================End of - Headers==============================




From: nyilmaz-at-mersin.edu.tr
Date: Thu, 13 Apr 2006 20:52:10 -0500
Subject: [Microscopy] viaWWW: Biofilm Ultrastructure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both nyilmaz-at-mersin.edu.tr as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: nyilmaz-at-mersin.edu.tr
Name: Necat Yilmaz

Organization: Mersin University School of Medicine Histology & Embryology Dep.

Title-Subject: [Filtered] Biofilm Ultrastructure...

Question: Hello...

We're planning to study staph aureus biofilms by TEM on a latex material like examination gloves. Does anybody know any method for it and is ruthenium red an obligation for processing or can we do it by conventional methods?
Thanks in advance...


---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Thu Apr 13 20:52:08 2006
8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3E1q6a9003379
8, 12 -- for {microscopy-at-microscopy.com} ; Thu, 13 Apr 2006 20:52:07 -0500
8, 12 -- Mime-Version: 1.0
8, 12 -- X-Sender: (Unverified)
8, 12 -- Message-Id: {p06110401c064b0b6f654-at-[206.69.208.22]}
8, 12 -- Date: Thu, 13 Apr 2006 20:52:04 -0500
8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: nyilmaz-at-mersin.edu.tr (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: Biofilm Ultrastructure
8, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: robn-at-hyperbranch.com
Date: Fri, 14 Apr 2006 07:28:33 -0500
Subject: [Microscopy] viaWWW: cyro-SEM work

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both robn-at-hyperbranch.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: robn-at-hyperbranch.com
Name: Rob Naslund

Organization: hyperbranch medical technology

Title-Subject: [Filtered] SEM - cryo-SEM

Question: I am looking for a contract lab to do some cyro-SEM work on hydrogels. Any suggestions?

Thanks

---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Fri Apr 14 07:28:32 2006
7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3ECSWJi023757
7, 12 -- for {microscopy-at-microscopy.com} ; Fri, 14 Apr 2006 07:28:32 -0500
7, 12 -- Mime-Version: 1.0
7, 12 -- X-Sender: (Unverified)
7, 12 -- Message-Id: {p06110400c06545dfecab-at-[206.69.208.22]}
7, 12 -- Date: Fri, 14 Apr 2006 07:28:32 -0500
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: robn-at-hyperbranch.com (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: cyro-SEM work
7, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: dsherman-at-purdue.edu
Date: Fri, 14 Apr 2006 08:21:59 -0500
Subject: [Microscopy] Commercial EM service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

We need to contact for-profit EM service providers (sample preparation and
imaging, not repair and maintenance) for a financial study related to core
facilities being conducted by a professor in our management school.

Would those providing these types of services please contact me off-line.

Many thanks,

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


==============================Original Headers==============================
8, 21 -- From dsherman-at-purdue.edu Fri Apr 14 08:21:59 2006
8, 21 -- Received: from exchange.purdue.edu (1061exfe04.adpc.purdue.edu [128.210.63.227])
8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3EDLxsa001703
8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 14 Apr 2006 08:21:59 -0500
8, 21 -- Received: from EXCH02.purdue.lcl ([128.210.63.235]) by exchange.purdue.edu with Microsoft SMTPSVC(6.0.3790.211);
8, 21 -- Fri, 14 Apr 2006 09:21:56 -0400
8, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH02.purdue.lcl ([128.210.63.237]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.239]) with Microsoft Exchange Server HTTP-DAV ;
8, 21 -- Fri, 14 Apr 2006 13:21:56 +0000
8, 21 -- User-Agent: Microsoft-Entourage/11.2.3.060209
8, 21 -- Date: Fri, 14 Apr 2006 09:21:56 -0400
8, 21 -- Subject: Commercial EM service
8, 21 -- From: Debby Sherman {dsherman-at-purdue.edu}
8, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
8, 21 -- Message-ID: {C0651A34.EE99%dsherman-at-purdue.edu}
8, 21 -- Thread-Topic: Commercial EM service
8, 21 -- Thread-Index: AcZfxmbUpRgsyMu5EdqNzQARJN08Mg==
8, 21 -- Mime-version: 1.0
8, 21 -- Content-type: text/plain;
8, 21 -- charset="US-ASCII"
8, 21 -- Content-transfer-encoding: 7bit
8, 21 -- X-OriginalArrivalTime: 14 Apr 2006 13:21:56.0962 (UTC) FILETIME=[67673C20:01C65FC6]
==============================End of - Headers==============================




From: cammer-at-aecom.yu.edu
Date: Fri, 14 Apr 2006 11:38:21 -0500
Subject: [Microscopy] Re: Digital Imaging in the SEM and absolute truth in imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

To put it simply, no image is objective.

Think the nonlinear responses of film/paper vs. the nonlinear responses of
video vs. PMT point scanning vs. linearity of CCDs vs. wide dynamic range
of 12+ bits. And what you see in your darkroom or monitor is not what the
printer will reproduce in halftone and variable inks on variable papers or
scanned or resized and compressed and thrown up on the web.

The two biggest way I deal with this are:
1. Running tests to characterize the imaging technique.
2. Forcing researchers to do control samples and image them and
postprocess them at the same settings etc. as the experimental. They
should be doing this anyhow. Usually the real question isn't an objective
absolute answer but variations with conditions and how this fits into the
bigger picture, e.g. compared with gene expression or blots for proteins or
whole animal behavior etc. The photo of the KO animal's brain is
meaningless without the companion photo of the normal animal's brain. Very
likely, it's not so important whether the image originated as a 640 X 480
pixels digitized from video, 35mm Tri-X pan printed on Ilford grade 4 paper
or a 1315 X 1000 pixel 12 bits highly linear CCD. Can we resolve the
structures under scrutiny and are there meaningful differences in the biology?

In philosophy and the arts there are enormous bodies of literature that
uniformly debunk the notion of absolute meanings in
photographs. Regardless of the physics and purity and sanctity of the
absolute object, we are interpreting beings.
____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
URL: http://www.aecom.yu.edu/aif/


==============================Original Headers==============================
5, 22 -- From cammer-at-aecom.yu.edu Fri Apr 14 11:38:20 2006
5, 22 -- Received: from mailgw.aecom.yu.edu (mailgw.aecom.yu.edu [129.98.1.16])
5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3EGcJdJ013734
5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 14 Apr 2006 11:38:20 -0500
5, 22 -- Received: from mailvx.aecom.yu.edu (mailvx.aecom.yu.edu [129.98.1.17])
5, 22 -- by mailgw.aecom.yu.edu (8.12.11.20060308/8.12.11) with SMTP id k3EGc9oo026389
5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 14 Apr 2006 12:38:18 -0400
5, 22 -- Received: from post.aecom.yu.edu ([129.98.1.100])
5, 22 -- by mailvx.aecom.yu.edu (SAVSMTP 3.1.1.32) with SMTP id M2006041412381813819
5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 14 Apr 2006 12:38:18 -0400
5, 22 -- Received: from AIF3.aecom.yu.edu (aif3.aif.aecom.yu.edu [129.98.30.137])
5, 22 -- by post.aecom.yu.edu (Postfix) with ESMTP id ADE102FD0
5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 14 Apr 2006 12:38:18 -0400 (EDT)
5, 22 -- Message-Id: {5.2.1.1.2.20060414123130.02a88fa8-at-mailserver.aecom.yu.edu}
5, 22 -- X-Sender: cammer-at-mailserver.aecom.yu.edu
5, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1
5, 22 -- Date: Fri, 14 Apr 2006 12:36:18 -0400
5, 22 -- To: microscopy-at-microscopy.com
5, 22 -- From: Michael Cammer {cammer-at-aecom.yu.edu}
5, 22 -- Subject: Re: Digital Imaging in the SEM and absolute truth in imaging
5, 22 -- Mime-Version: 1.0
5, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed
==============================End of - Headers==============================




From: pengw.william-at-gmail.com
Date: Sat, 15 Apr 2006 08:58:13 -0500
Subject: [Microscopy] viaWWW: Profile data export in DigitalMicrograph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both pengw.william-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: pengw.william-at-gmail.com
Name: William Peng

Organization: Tsinghua University

Title-Subject: [Filtered] Profile data export in DigitalMicrograph

Question: hello,all

I want to export multiple line profile data in Gatan DigitalMicrograph to Origin software. I can change the display type from line plot to spreadsheet in object menu. A spreadsheet is showed only have intensity value and no X value. Could I get a data file about this kind of line profile?

Thanks in advance.

William Peng
pengw.william-at-gmail.com
-----------------------------------------------
Department of Materials Science & Engineering,
Tsinghua University
China

---------------------------------------------------------------------------

==============================Original Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Sat Apr 15 08:58:13 2006
9, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
9, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3FDwCrb024227
9, 12 -- for {microscopy-at-microscopy.com} ; Sat, 15 Apr 2006 08:58:13 -0500
9, 12 -- Mime-Version: 1.0
9, 12 -- X-Sender: (Unverified)
9, 12 -- Message-Id: {p06110401c066ac51f109-at-[206.69.208.22]}
9, 12 -- Date: Sat, 15 Apr 2006 08:58:11 -0500
9, 12 -- To: microscopy-at-microscopy.com
9, 12 -- From: pengw.william-at-gmail.com (by way of MicroscopyListserver)
9, 12 -- Subject: viaWWW: Profile data export in DigitalMicrograph
9, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: cgarber-at-2spi.com
Date: Sun, 16 Apr 2006 17:26:50 -0500
Subject: [Microscopy] Cryo-SEM on hydrogels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Rob Naslund wrote:
================================================================
Question: I am looking for a contract lab to do some cyro-SEM work on hydrogels. Any suggestions?
================================================================
Structure Probe, Inc has an Oxford cryo-SEM system interfaced to a JEOL Model 840 SEM. We have had experience characterizing hydrogels by this approach. Part of our firm is an independent analytical laboratory.

Contact me off-line for a proposal.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
Structure Probe, Inc. FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: www.2spi.com
############################
==================================================








==============================Original Headers==============================
13, 29 -- From cgarber-at-2spi.com Sun Apr 16 17:26:50 2006
13, 29 -- Received: from s-utl01-sjpop.stsn.net (s-utl01-sjpop.stsn.net [72.254.0.201])
13, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k3GMQnKb019913
13, 29 -- for {microscopy-at-msa.microscopy.com} ; Sun, 16 Apr 2006 17:26:49 -0500
13, 29 -- Received: from s-utl01-sjpop.stsn.net ([127.0.0.1])
13, 29 -- by s-utl01-sjpop.stsn.net (SMSSMTP 4.1.2.20) with SMTP id M2006041615264724786
13, 29 -- ; Sun, 16 Apr 2006 15:26:47 -0700
13, 29 -- X-Spam-Status: No, hits=0.0 required=9.9
13, 29 -- tests=ALL_TRUSTED: -2.867,AWL: 0.105,BAYES_00: -1.665,
13, 29 -- DATE_IN_PAST_03_06: 0.127,SARE_RECV_ADDR: 0.027
13, 29 -- X-Spam-Level:
13, 29 -- Received: from ibm1x23g2abfyg ([10.1.185.112])
13, 29 -- by s-utl01-sjpop.stsn.net;
13, 29 -- Sun, 16 Apr 2006 15:26:46 -0700
13, 29 -- Message-ID: {001601c661a4$d37615a0$70b9010a-at-ibm1x23g2abfyg}
13, 29 -- Reply-To: "Garber, Charles A." {cgarber-at-2spi.com}
13, 29 -- From: "Garber, Charles A." {cgarber-at-2spi.com}
13, 29 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com}
13, 29 -- Subject: Cryo-SEM on hydrogels
13, 29 -- Date: Sun, 16 Apr 2006 13:31:21 -0400
13, 29 -- MIME-Version: 1.0
13, 29 -- Content-Type: text/plain;
13, 29 -- charset="Windows-1252"
13, 29 -- X-Priority: 3
13, 29 -- X-MSMail-Priority: Normal
13, 29 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2869
13, 29 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2869
13, 29 -- Content-Transfer-Encoding: 8bit
13, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3GMQnKb019913
==============================End of - Headers==============================




From: pedromfjcosta-at-gmail.com
Date: Mon, 17 Apr 2006 17:57:55 -0500
Subject: [Microscopy] viaWWW: Grease for ion millers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

"Selecting a CCD Camera for Light Microscopy," a live, informative, and
interactive web-based seminar is being held this Friday (21-April) at 11:30
AM (New York time).

Details are below. There is no cost, but connection lines are limited so
reserve yours now.

--------------------------------------------------------------
TO RESERVE YOUR CONNECTION LINE
--------------------------------------------------------------
Click this URL:

https://premconf.webex.com/premconf/j.php?ED=86778962&RG=1

Click REGISTER and complete the requested information. You will be sent a
link that gives you access to Friday's meeting.

----------------------------------------
MEETING SUMMARY
----------------------------------------
Name: Selecting a CCD Camera for Light Microscopy

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both pedromfjcosta-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: pedromfjcosta-at-gmail.com
Name: Pedro MFJ Costa

Organization: University of Cambridge

Title-Subject: [Filtered] Grease for ion millers

Question: We have a Gatan PIPS ion miller in our lab which periodically needs maintenance of the O-rings. For sealing and lubricant purposes we usually use Fomblin Vac3 grease. However, we find that it tends to be a bit too sticky for O-rings of moving parts and forces us to be often cleaning the chamber shutter.
I would be grateful for suggestions on which is the best silicon-free grease to use in the Gatan PIPS, particularly as lubricant.


---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Mon Apr 17 17:57:55 2006
7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3HMvsoG014454
7, 12 -- for {microscopy-at-microscopy.com} ; Mon, 17 Apr 2006 17:57:55 -0500
7, 12 -- Mime-Version: 1.0
7, 12 -- X-Sender: (Unverified)
7, 12 -- Message-Id: {p06110401c069cdddd1bd-at-[206.69.208.22]}
7, 12 -- Date: Mon, 17 Apr 2006 17:57:54 -0500
7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: pedromfjcosta-at-gmail.com (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: Grease for ion millers
7, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: cgarber-at-2spi.com
Date: Tue, 18 Apr 2006 04:04:05 -0500
Subject: [Microscopy] Grease for ion millers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Pedro MFJ Costa wrote:
===========================================================
Question: We have a Gatan PIPS ion miller in our lab which periodically needs maintenance of the O-rings. For sealing and lubricant purposes we usually use Fomblin Vac3 grease. However, we find that it tends to be a bit too sticky for O-rings of moving parts and forces us to be often cleaning the chamber shutter.
I would be grateful for suggestions on which is the best silicon-free grease to use in the Gatan PIPS, particularly as lubricant
===========================================================
You are presently using a PFPE (perfluorinated polyether) grease. It is a two component system, PTFE particles dispersed in a perfluorinated liquid continuous phase. Although the vapor pressure of the liquid phase is quite low, and is in the range of a diffusion pump fluid, it does eventually "disappear".

Braycote Micronic 803 is similar in characteristics but the grease is filtered through a screen pack filter to remove any agglomerates of the PTFE particles larger than 1 um. It is a more homogeneous lubricant. It would seem that one could expect this Braycote product to perform more to your satisfaction for the above cited reasons. You can find out more about Braycote Micronic 803 at URL
http://www.2spi.com/catalog/vac/braycote-micronic-803.shtml

Chuck

PS: Remember that we are striving to be 100% paperless, therefore there
are no paper copies kept of this correspondence. Please be sure to always
reply by way of "reply" on your software so that the entire string of
correspondence can be kept in one place.
===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================





.





==============================Original Headers==============================
15, 30 -- From cgarber-at-2spi.com Tue Apr 18 04:04:05 2006
15, 30 -- Received: from s-utl01-sjpop.stsn.net (s-utl01-sjpop.stsn.net [72.254.0.201])
15, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k3I944ja000489
15, 30 -- for {microscopy-at-msa.microscopy.com} ; Tue, 18 Apr 2006 04:04:05 -0500
15, 30 -- Received: from s-utl01-sjpop.stsn.net ([127.0.0.1])
15, 30 -- by s-utl01-sjpop.stsn.net (SMSSMTP 4.1.2.20) with SMTP id M2006041802040430529
15, 30 -- for {microscopy-at-msa.microscopy.com} ; Tue, 18 Apr 2006 02:04:04 -0700
15, 30 -- X-Spam-Status: No, hits=0.0 required=9.9
15, 30 -- tests=ALL_TRUSTED: -2.867,AWL: 0.101,BAYES_00: -1.665,
15, 30 -- DATE_IN_PAST_03_06: 0.127,SARE_RECV_ADDR: 0.027
15, 30 -- X-Spam-Level:
15, 30 -- Received: from ibm1x23g2abfyg ([10.1.185.112])
15, 30 -- by s-utl01-sjpop.stsn.net
15, 30 -- for microscopy-at-msa.microscopy.com;
15, 30 -- Tue, 18 Apr 2006 02:04:04 -0700
15, 30 -- Message-ID: {001101c662c7$065beee0$70b9010a-at-ibm1x23g2abfyg}
15, 30 -- Reply-To: "Garber, Charles A." {cgarber-at-2spi.com}
15, 30 -- From: "Garber, Charles A." {cgarber-at-2spi.com}
15, 30 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com}
15, 30 -- Subject: Grease for ion millers
15, 30 -- Date: Mon, 17 Apr 2006 23:09:34 -0400
15, 30 -- MIME-Version: 1.0
15, 30 -- Content-Type: text/plain;
15, 30 -- charset="Windows-1252"
15, 30 -- X-Priority: 3
15, 30 -- X-MSMail-Priority: Normal
15, 30 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2869
15, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2869
15, 30 -- Content-Transfer-Encoding: 8bit
15, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3I944ja000489
==============================End of - Headers==============================




From: microscopytoday-at-tampabay.rr.com
Date: Tue, 18 Apr 2006 08:52:40 -0500
Subject: [Microscopy] Re: viaWWW: Tripod Polisher micrometers locking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Pedro,

The locked micrometers are probably toast. When you get new micrometers,
or if you want to protect ones that aren't rusted, unscrew the barrels
and rub the threads with real lanolin, not a chemical substitute.
Lanolin can be purchased in most drugstores. Sheep swear by the stuff!
Works for tripod polisher micrometers and the turnbuckles on my
sailboat, which are exposed to salt water.

Ron Anderson

pedromfjcosta-at-gmail.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both pedromfjcosta-at-gmail.com as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: pedromfjcosta-at-gmail.com
} Name: Pedro MFJ Costa
}
} Organization: University of Cambridge
}
} Title-Subject: [Filtered] Tripod Polisher micrometers locking
}
} Question: We have several tripod polishers in your lab and, unfortunately, we find that with time the precision micrometers tend to get locked up. In fact, despite trying to avoid excessive water infiltrating the micrometers they still become rusty.
}
} I was wondering if anyone had a way to prevent this from happening or possibly to unlock them without incurring into further damage.
}
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 12 -- From zaluzec-at-microscopy.com Mon Apr 10 23:44:01 2006
} 8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
} 8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3B4i0Dm027217
} 8, 12 -- for {microscopy-at-microscopy.com} ; Mon, 10 Apr 2006 23:44:00 -0500
} 8, 12 -- Mime-Version: 1.0
} 8, 12 -- X-Sender: (Unverified)
} 8, 12 -- Message-Id: {p06110403c060e47f14e0-at-[206.69.208.22]}
} 8, 12 -- Date: Mon, 10 Apr 2006 23:43:57 -0500
} 8, 12 -- To: microscopy-at-microscopy.com
} 8, 12 -- From: pedromfjcosta-at-gmail.com (by way of MicroscopyListserver)
} 8, 12 -- Subject: viaWWW: Tripod Polisher micrometers locking
} 8, 12 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of - Headers==============================
}
}
}


==============================Original Headers==============================
5, 19 -- From microscopytoday-at-tampabay.rr.com Tue Apr 18 08:52:40 2006
5, 19 -- Received: from ms-smtp-04.tampabay.rr.com (ms-smtp-04.tampabay.rr.com [65.32.5.134])
5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3IDqdrp018634
5, 19 -- for {Microscopy-at-Microscopy.Com} ; Tue, 18 Apr 2006 08:52:40 -0500
5, 19 -- Received: from [127.0.0.1] (rrcs-24-73-73-214.se.biz.rr.com [24.73.73.214])
5, 19 -- by ms-smtp-04.tampabay.rr.com (8.13.4/8.13.4) with ESMTP id k3IDqZwf020552;
5, 19 -- Tue, 18 Apr 2006 09:52:38 -0400 (EDT)
5, 19 -- Message-ID: {4444EF20.8040801-at-tampabay.rr.com}
5, 19 -- Date: Tue, 18 Apr 2006 09:52:32 -0400
5, 19 -- From: Microscopy Today {microscopytoday-at-tampabay.rr.com}
5, 19 -- User-Agent: Thunderbird 1.5 (Windows/20051201)
5, 19 -- MIME-Version: 1.0
5, 19 -- To: pedromfjcosta-at-gmail.com, Listserver {Microscopy-at-Microscopy.Com}
5, 19 -- Subject: Re: [Microscopy] viaWWW: Tripod Polisher micrometers locking
5, 19 -- References: {200604110444.k3B4iL5C027432-at-ns.microscopy.com}
5, 19 -- In-Reply-To: {200604110444.k3B4iL5C027432-at-ns.microscopy.com}
5, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
5, 19 -- Content-Transfer-Encoding: 7bit
5, 19 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine
==============================End of - Headers==============================




From: kenconverse-at-qualityimages.biz
Date: Tue, 18 Apr 2006 10:46:46 -0500
Subject: [Microscopy] Grease for ion millers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Pedro,
My experience is that Braycote 803 works great for static seals but, like
the Fomblin, becomes sticky (grabs) overnight. Braycote 602 is a different
formulation with about 2 decades lower vapor pressure with moly disulfide
included as a lubricant. It seems to be much better for dynamic seals.
It's also a lot more expensive, but IMHO is worth it. I believe Chuck has
it or can get it.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: cgarber-at-2spi.com [mailto:cgarber-at-2spi.com]
Sent: Tuesday, April 18, 2006 5:11 AM
To: kenconverse-at-qualityimages.biz

Pedro MFJ Costa wrote:
===========================================================
Question: We have a Gatan PIPS ion miller in our lab which periodically
needs maintenance of the O-rings. For sealing and lubricant purposes we
usually use Fomblin Vac3 grease. However, we find that it tends to be a bit
too sticky for O-rings of moving parts and forces us to be often cleaning
the chamber shutter.
I would be grateful for suggestions on which is the best silicon-free grease
to use in the Gatan PIPS, particularly as lubricant
===========================================================
You are presently using a PFPE (perfluorinated polyether) grease. It is a
two component system, PTFE particles dispersed in a perfluorinated liquid
continuous phase. Although the vapor pressure of the liquid phase is quite
low, and is in the range of a diffusion pump fluid, it does eventually
"disappear".

Braycote Micronic 803 is similar in characteristics but the grease is
filtered through a screen pack filter to remove any agglomerates of the PTFE
particles larger than 1 um. It is a more homogeneous lubricant. It would
seem that one could expect this Braycote product to perform more to your
satisfaction for the above cited reasons. You can find out more about
Braycote Micronic 803 at URL
http://www.2spi.com/catalog/vac/braycote-micronic-803.shtml

Chuck

PS: Remember that we are striving to be 100% paperless, therefore there
are no paper copies kept of this correspondence. Please be sure to always
reply by way of "reply" on your software so that the entire string of
correspondence can be kept in one place.
===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================





.





==============================Original Headers==============================
15, 30 -- From cgarber-at-2spi.com Tue Apr 18 04:04:05 2006
15, 30 -- Received: from s-utl01-sjpop.stsn.net (s-utl01-sjpop.stsn.net
[72.254.0.201])
15, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id
k3I944ja000489
15, 30 -- for {microscopy-at-msa.microscopy.com} ; Tue, 18 Apr 2006
04:04:05 -0500
15, 30 -- Received: from s-utl01-sjpop.stsn.net ([127.0.0.1])
15, 30 -- by s-utl01-sjpop.stsn.net (SMSSMTP 4.1.2.20) with SMTP id
M2006041802040430529
15, 30 -- for {microscopy-at-msa.microscopy.com} ; Tue, 18 Apr 2006 02:04:04
-0700
15, 30 -- X-Spam-Status: No, hits=0.0 required=9.9
15, 30 -- tests=ALL_TRUSTED: -2.867,AWL: 0.101,BAYES_00: -1.665,
15, 30 -- DATE_IN_PAST_03_06: 0.127,SARE_RECV_ADDR: 0.027
15, 30 -- X-Spam-Level:
15, 30 -- Received: from ibm1x23g2abfyg ([10.1.185.112])
15, 30 -- by s-utl01-sjpop.stsn.net
15, 30 -- for microscopy-at-msa.microscopy.com;
15, 30 -- Tue, 18 Apr 2006 02:04:04 -0700
15, 30 -- Message-ID: {001101c662c7$065beee0$70b9010a-at-ibm1x23g2abfyg}
15, 30 -- Reply-To: "Garber, Charles A." {cgarber-at-2spi.com}
15, 30 -- From: "Garber, Charles A." {cgarber-at-2spi.com}
15, 30 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com}
15, 30 -- Subject: Grease for ion millers
15, 30 -- Date: Mon, 17 Apr 2006 23:09:34 -0400
15, 30 -- MIME-Version: 1.0
15, 30 -- Content-Type: text/plain;
15, 30 -- charset="Windows-1252"
15, 30 -- X-Priority: 3
15, 30 -- X-MSMail-Priority: Normal
15, 30 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2869
15, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2869
15, 30 -- Content-Transfer-Encoding: 8bit
15, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by
ns.microscopy.com id k3I944ja000489
==============================End of - Headers==============================





___________________________________________________________
$0 Web Hosting with up to 200MB web space, 1000 MB Transfer
10 Personalized POP and Web E-mail Accounts, and much more.
Signup at www.doteasy.com



==============================Original Headers==============================
31, 24 -- From kenconverse-at-qualityimages.biz Tue Apr 18 10:46:46 2006
31, 24 -- Received: from qualityimages.biz (dpmail16.doteasy.com [65.61.209.16])
31, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3IFkjR9031673
31, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 18 Apr 2006 10:46:46 -0500
31, 24 -- Received: from Ken [68.64.242.101] by qualityimages.biz with ESMTP
31, 24 -- (SMTPD32-8.05) id A9C9280800BE; Tue, 18 Apr 2006 08:46:17 -0700
31, 24 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz}
31, 24 -- To: {cgarber-at-2spi.com} , {pedromfjcosta-at-gmail.com} ,
31, 24 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com}
31, 24 -- Subject: RE: [Microscopy] Grease for ion millers
31, 24 -- Date: Tue, 18 Apr 2006 11:46:11 -0400
31, 24 -- Message-ID: {001201c662ff$3d4f7d80$6401a8c0-at-Ken}
31, 24 -- MIME-Version: 1.0
31, 24 -- Content-Type: text/plain;
31, 24 -- charset="us-ascii"
31, 24 -- X-Priority: 3 (Normal)
31, 24 -- X-MSMail-Priority: Normal
31, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.6626
31, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2869
31, 24 -- Importance: Normal
31, 24 -- In-Reply-To: {200604180910.k3I9Aln0005869-at-ns.microscopy.com}
31, 24 -- X-IMSTrailer: __IMail_7__
31, 24 -- Content-Transfer-Encoding: 8bit
31, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3IFkjR9031673
==============================End of - Headers==============================




From: Mark.Clark-at-atdf.com
Date: Tue, 18 Apr 2006 11:06:32 -0500
Subject: [Microscopy] TEM Analyst Position Open at SEMATECH/ATDF in Austin, Texas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

A position is open for a Transmission Electron Microscopy Analyst in the Process Characterization Group at ATDF (www.atdf.com), a wholly-owned subsidiary of SEMATECH) in Austin, Texas.

Our group provides technical support to research and development projects for SEMATECH (www.sematech.org), ATDF, and ATDF customer funded projects in the field of semiconductor and nanomaterials development. The analyst would interface with engineers and project managers, determine analytical plans, conduct analyses, and interpret and present data. The work involves characterization of materials systems new to the semiconductor industry and there is significant opportunity to do interesting and publishable science. Our business plan allows significant opportunities for collaboration and visibility within the industry as well as the materials and microscopy academic communities.

Our toolset includes two 300 keV TEM's (one FEG, one LaB6), with SUTW-EDAX EDXS, GIF-2001 EELS, multiple HAADF-STEM and CCD cameras, and a biprism paired with a Lorentz lens. Our sample preparation toolset includes several broad-beam ion mills as well as 2 focused ion beam systems.

The optimal candidate should hold a PhD in Materials Science, Chemistry or Physics, plus have several years experience in TEM characterization of semiconductor materials. A strong understanding of wafer processing is favorable.

The successful candidate should look forward to becoming an important part of our analytical team, working to solve problems, publish and present at technical conferences, lead internal teams and act in the role as a mentor to support the development of others within our group.

Interested parties should send their CV by email to Mark.Clark-at-atdf.com



==============================Original Headers==============================
8, 41 -- From Mark.Clark-at-atdf.com Tue Apr 18 11:06:31 2006
8, 41 -- Received: from outbound2-kan-R.bigfish.com (outbound-kan.frontbridge.com [63.161.60.23])
8, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3IG6VfM009275
8, 41 -- for {Microscopy-at-Microscopy.Com} ; Tue, 18 Apr 2006 11:06:31 -0500
8, 41 -- Received: from outbound2-kan.bigfish.com (localhost.localdomain [127.0.0.1])
8, 41 -- by outbound2-kan-R.bigfish.com (Postfix) with ESMTP id 7213044C913
8, 41 -- for {Microscopy-at-Microscopy.Com} ; Tue, 18 Apr 2006 16:06:31 +0000 (UTC)
8, 41 -- Received: from mail4-kan-R.bigfish.com (unknown [172.18.7.1])
8, 41 -- by outbound2-kan.bigfish.com (Postfix) with ESMTP id 6AC7544C9DD
8, 41 -- for {Microscopy-at-Microscopy.Com} ; Tue, 18 Apr 2006 16:06:31 +0000 (UTC)
8, 41 -- Received: from mail4-kan.bigfish.com (localhost.localdomain [127.0.0.1])
8, 41 -- by mail4-kan-R.bigfish.com (Postfix) with ESMTP id 5F1AF377D15
8, 41 -- for {Microscopy-at-Microscopy.Com} ; Tue, 18 Apr 2006 16:06:31 +0000 (UTC)
8, 41 -- X-BigFish: VP
8, 41 -- Received: by mail4-kan (MessageSwitch) id 1145376391329369_22560; Tue, 18 Apr 2006 16:06:31 +0000 (UCT)
8, 41 -- Received: from mitch.eng.sematech.org (GATER5.SEMATECH.ORG [192.73.53.5])
8, 41 -- by mail4-kan.bigfish.com (Postfix) with ESMTP id 4734B376DCD
8, 41 -- for {Microscopy-at-Microscopy.Com} ; Tue, 18 Apr 2006 16:06:31 +0000 (UTC)
8, 41 -- Received: from CONVERSION-DAEMON.SEMATECH.Org by SEMATECH.Org
8, 41 -- (PMDF V6.2-X26 #31211) id {01M1EG5EO8KW00VB0A-at-SEMATECH.Org} for
8, 41 -- Microscopy-at-Microscopy.Com; Tue, 18 Apr 2006 11:06:30 -0500 (CDT)
8, 41 -- Received: from cod.logon.sematech.org (cod.logon.sematech.org [131.153.32.40])
8, 41 -- by SEMATECH.Org (PMDF V6.2-X26 #31211)
8, 41 -- with ESMTP id {01M1EG5EC7AW00VGBP-at-SEMATECH.Org} for Microscopy-at-Microscopy.Com;
8, 41 -- Tue, 18 Apr 2006 11:06:30 -0500 (CDT)
8, 41 -- Date: Tue, 18 Apr 2006 11:06:29 -0500
8, 41 -- From: Mark.Clark-at-atdf.com
8, 41 -- Subject: TEM Analyst Position Open at SEMATECH/ATDF in Austin, Texas
8, 41 -- To: Microscopy-at-Microscopy.Com
8, 41 -- Cc: Celina.Ortiz-at-atdf.com
8, 41 -- Message-id: {59157C31AE9B264D8CB608D6026FE53E016E3425-at-cod.logon.sematech.org}
8, 41 -- MIME-version: 1.0
8, 41 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5.7226.0
8, 41 -- Content-type: text/plain; charset=iso-8859-1
8, 41 -- Thread-Topic: TEM Analyst Position Open at SEMATECH/ATDF in Austin, Texas
8, 41 -- Thread-Index: AcZjAg2uUy4ImXZWSK+uFP9g/A6ReQ==
8, 41 -- Content-class: urn:content-classes:message
8, 41 -- X-MS-Has-Attach:
8, 41 -- X-MS-TNEF-Correlator:
8, 41 -- Content-Transfer-Encoding: 8bit
8, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3IG6VfM009275
==============================End of - Headers==============================




From: jmkrupp-at-ucsc.edu
Date: Tue, 18 Apr 2006 12:58:44 -0500
Subject: [Microscopy] Hitachi S-2700 LaB6 instructions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

I have just inherited a Hitachi S-2700 LaB6 SEM. It came from an
industrial setting and some of the instructions were missing. I think
they were in a desk in the room with the microscope and when the
company wanted to vacate the space ASAP the movers shoved out all the
easy to move furniture, including the desk with the instructions and
specimen holders, but they couldn't budge the microscope so it stayed
there until I picked it up.

So, I have the instructions for a tungsten filament unit, but this
one is clearly a LaB6 guy. I need a clue about how to open the gun,
set the tip, and if there are any operating changes for routine use.

Anybody got anything that would help?

The specimen holder stuff is all missing too. From the W instruction
book I can see that there was supposed to be a jig for adjusting
specimen height and some special intermediate pieces to mate the stub
with the stage. I have never had a Hitachi SEM before, so I and not
familiar with their system, any hints or clues would be helpful.

Thanks

Jon

--
I'm riding in the MS 150 Crusin' to the Coast bike ride May 13 - 14,
2006 to raise money for the National Multiple Sclerosis Society. You
can go to http://www.msconnection.org and follow the links to make an
ePledge if you wish.


Jonathan Krupp
Microscopy & Imaging Lab
C230 Earth & Marine Science
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-ucsc.edu

==============================Original Headers==============================
10, 21 -- From jmkrupp-at-ucsc.edu Tue Apr 18 12:58:43 2006
10, 21 -- Received: from smtp-prod-mx2.ucsc.edu (smtp-prod-mx2.ucsc.edu [128.114.125.44])
10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3IHwhQ1020869
10, 21 -- for {microscopy-at-microscopy.com} ; Tue, 18 Apr 2006 12:58:43 -0500
10, 21 -- Received: from ucsc.edu (cruzmail-fe2.ucsc.edu [128.114.125.2])
10, 21 -- by smtp-prod-mx2.ucsc.edu (8.13.6/8.13.1) with ESMTP id k3IHwXCe013575
10, 21 -- for {microscopy-at-microscopy.com} ; Tue, 18 Apr 2006 10:58:33 -0700 (PDT)
10, 21 -- Received: from [128.114.25.185] (account jmkrupp-at-ucsc.edu [128.114.25.185] verified)
10, 21 -- by silver.ucsc.edu (CommuniGate Pro SMTP 4.3.7)
10, 21 -- with ESMTPA id 52946994 for microscopy-at-microscopy.com; Tue, 18 Apr 2006 10:58:33 -0700
10, 21 -- Mime-Version: 1.0
10, 21 -- Message-Id: {p06230903c06ad716f8c0-at-[128.114.25.185]}
10, 21 -- Date: Tue, 18 Apr 2006 10:58:32 -0700
10, 21 -- To: microscopy-at-microscopy.com
10, 21 -- From: Jon Krupp {jmkrupp-at-ucsc.edu}
10, 21 -- Subject: Hitachi S-2700 LaB6 instructions
10, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
10, 21 -- X-UCSC-CATS-Information: This message was scanned by the ITS MailScanner
10, 21 -- X-UCSC-CATS-MailScanner: Found to be clean
10, 21 -- X-UCSC-CATS-MailScanner-SpamCheck:
10, 21 -- X-UCSC-CATS-MailScanner-From: jmkrupp-at-ucsc.edu
==============================End of - Headers==============================




From: Don.Becker-at-bruker-axs.com
Date: Tue, 18 Apr 2006 13:06:28 -0500
Subject: [Microscopy] Employment Opportunity at Bruker AXS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Bruker AXS Inc., a leading global provider of advanced X-ray solutions
for the life and advanced materials sciences, is seeking an experienced

Sr. Sales Engineer - Microanalysis
Southeast Territory

Secure sales for BAXS Microanalysis Products in the southeast U.S. and
act as company rep to existing customer base and prospective customers.
Responsibilities include: prospecting for new customers, following up
sales leads provided by the company, presenting and demonstrating
company products, supplying quotations and technical information,
formulating sales strategies, as well as negotiating and securing sales
orders. In addition, the Regional Sales Manager will maintain contact
with existing customers to assure their satisfaction, develop good
working relationships with OEM salespeople, collect and report market
information and provide routine sales forecasts.

Territory includes NC, SC, GA, FL, TN, AL, MS, AR, and LA. At least 50%
travel is required. Bachelor's degree (B.S.or B.A.) from four-year
college or university; or five years experience and/or training; or
equivalent combination of education and experience. Three to five years
experience in scientific equipment sales is a must. This position
requires excellent verbal communication and interaction skills.

Bruker AXS Inc. offers a competitive salary and comprehensive benefits
package including health and dental insurance, Flexible Spending
Account, company sponsored life and disability insurance, 401(k) plan
with company matching components, stock option plan, and vacation and
sick/personal days.

Qualified applicants should submit resume and salary requirements in
confidence to:

Bruker AXS Inc.
Attn: Don Becker, Sales Manager
1239 Parkway Ave. Suite 203
Ewing, NJ 08628
FAX#: 609-771-4411
E-mail: don.becker-at-bruker-axs.com

Equal Opportunity Employer




==============================Original Headers==============================
10, 23 -- From Don.Becker-at-bruker-axs.com Tue Apr 18 13:06:28 2006
10, 23 -- Received: from isa1.bruker-axs.com (host-216-153-173-238.mil.choiceone.net [216.153.173.238])
10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3II6QWu030668
10, 23 -- for {microscopy-at-microscopy.com} ; Tue, 18 Apr 2006 13:06:28 -0500
10, 23 -- Received: from mail1.bruker-axs.com ([172.16.0.32]) by isa1.bruker-axs.com with Microsoft SMTPSVC(6.0.3790.211);
10, 23 -- Tue, 18 Apr 2006 13:05:47 -0500
10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0
10, 23 -- Content-class: urn:content-classes:message
10, 23 -- MIME-Version: 1.0
10, 23 -- Content-Type: text/plain;
10, 23 -- charset="us-ascii"
10, 23 -- Subject: Employment Opportunity at Bruker AXS
10, 23 -- Date: Tue, 18 Apr 2006 13:05:46 -0500
10, 23 -- Message-ID: {235D0F9F0400B3449E8C229D9D24CC570242EDD5-at-mail1.bruker-axs.com}
10, 23 -- X-MS-Has-Attach:
10, 23 -- X-MS-TNEF-Correlator:
10, 23 -- Thread-Topic: Employment Opportunity at Bruker AXS
10, 23 -- Thread-Index: AcZjEreJTLN9eJJZQ16cOHsBpPFsSg==
10, 23 -- From: "Becker, Don" {Don.Becker-at-bruker-axs.com}
10, 23 -- To: {microscopy-at-microscopy.com}
10, 23 -- X-OriginalArrivalTime: 18 Apr 2006 18:05:47.0345 (UTC) FILETIME=[B7F47410:01C66312]
10, 23 -- Content-Transfer-Encoding: 8bit
10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3II6QWu030668
==============================End of - Headers==============================




From: kellie.l.garner-at-monsanto.com
Date: Tue, 18 Apr 2006 14:32:14 -0500
Subject: [Microscopy] LM paraffin section methods troubleshoot

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
Can someone please give me paraffin-sectioning advice? I am having problems
getting the paraffin to not split when I am sectioning. The sections are
supposed to be done at 8 microns. I have tried cooling the block, knife and
tweezers, still the sections split sometimes down the middle other times at
the sides. Today I heated the block a bit, and also tried 10 microns still
the sections are splitting.
Please HELP !!
Thank you,
Kellie

---------------------------------------------------------------------------------------------------------
This e-mail message may contain privileged and/or confidential information, and is intended to be received only by persons entitled to receive such information. If you have received this e-mail in error, please notify the sender immediately. Please delete it and all attachments from any servers, hard drives or any other media. Other use of this e-mail by you is strictly prohibited.


All e-mails and attachments sent and received are subject to monitoring, reading and archival by Monsanto. The recipient of this e-mail is solely responsible for checking for the presence of "Viruses" or other "Malware". Monsanto accepts no liability for any damage caused by any such code transmitted by or accompanying this e-mail or any attachment.
---------------------------------------------------------------------------------------------------------


==============================Original Headers==============================
5, 25 -- From kellie.l.garner-at-monsanto.com Tue Apr 18 14:32:14 2006
5, 25 -- Received: from gateway1.monsanto.com (gateway1.monsanto.com [199.89.234.134])
5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3IJWD7w010177
5, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Apr 2006 14:32:14 -0500
5, 25 -- Received: from agstlsmtp03.monsanto.com (agstlsmtp03.email.monsanto.com [10.30.65.106])
5, 25 -- by gateway1.monsanto.com (Switch-3.1.8/Switch-3.1.7) with ESMTP id k3IJWBrH003099
5, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Apr 2006 14:32:11 -0500 (CDT)
5, 25 -- thread-index: AcZjH0YzOS+sUgv2TgGWKHc9li8/7A==
5, 25 -- Received: from agstlhub02.monsanto.com ([10.30.64.99]) by agstlsmtp03.monsanto.com with Microsoft SMTPSVC(6.0.3790.1830); Tue, 18 Apr 2006 14:35:39 -0500
5, 25 -- Received: by agstlhub02.monsanto.com with Internet Mail Service (5.5.2653.19) id {J1VW8DGZ} ; Tue, 18 Apr 2006 14:28:21 -0500
5, 25 -- Message-ID: {30E69DC92141D14D93FAE0EC43BF9EEE0634A6-at-NA1000EXM02.na.ds.monsanto.com}
5, 25 -- From: "GARNER, KELLIE L [AG-Contractor/1000]" {kellie.l.garner-at-monsanto.com}
5, 25 -- To: {Microscopy-at-microscopy.com}
5, 25 -- Content-Transfer-Encoding: 7bit
5, 25 -- Subject: LM paraffin section methods troubleshoot
5, 25 -- Date: Tue, 18 Apr 2006 14:32:00 -0500
5, 25 -- MIME-Version: 1.0
5, 25 -- Content-Class: urn:content-classes:message
5, 25 -- X-Mailer: Internet Mail Service (5.5.2653.19)
5, 25 -- Importance: normal
5, 25 -- Priority: normal
5, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.2663
5, 25 -- Content-Type: text/plain;
5, 25 -- charset="iso-8859-1"
5, 25 -- X-OriginalArrivalTime: 18 Apr 2006 19:35:39.0734 (UTC) FILETIME=[4611B760:01C6631F]
==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Tue, 18 Apr 2006 15:05:38 -0500
Subject: [Microscopy] Re: LM paraffin section methods troubleshoot

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Kelly,

are the splits along a tissue-wax interface? If yes, then your
infiltration isn't complete.

Are you sure that you aren't leaving any tiny bits of razor blade or
other contaminants when you trim the face prior to sectioning?

Do the splits always occur at the same position ON THE KNIFE? If
yes, what type of knife are you using? If its disposable, dispose of
it and try a new one. If its the kind you resharpen, then it needs
to be resharpened. Even very tiny flaws in the knife edge can cause
scratches on the block face that translate to splits in the sections.

Can you adjust the clearance angle? The block could be rubbing
against the knife holder/stage once it sweeps past the knife edge
itself.

I know this sounds silly, but are you using the correct chuck holder?
It happened in my lab. My technician was using the chuck holder
designed for square blocks (as from Peel-Away molds) to clamp the
holder for the TissueTek bases. This resulted in the block extending
too far forward from the arm and rubbing against the knife stage.
She was frustrated by the scratched/splits in her sections, but no
one noticed the mistake until we had the microtome serviced and the
service guy asked us why we were doing that!

Those are my ideas. I'm sure you'll get others.
Good luck,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

==============================Original Headers==============================
7, 24 -- From lcgould-at-med.cornell.edu Tue Apr 18 15:05:38 2006
7, 24 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45])
7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3IK5cvG020895
7, 24 -- for {microscopy-at-microscopy.com} ; Tue, 18 Apr 2006 15:05:38 -0500
7, 24 -- Received: from mpx1.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120])
7, 24 -- by smtp-gw2.med.cornell.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id k3IK5b6p006081
7, 24 -- for {microscopy-at-microscopy.com} ; Tue, 18 Apr 2006 16:05:37 -0400 (EDT)
7, 24 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23])
7, 24 -- by mpx1.med.cornell.edu
7, 24 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005))
7, 24 -- with ESMTPA id {0IXX00DCXP5B6B60-at-mpx1.med.cornell.edu} for
7, 24 -- microscopy-at-microscopy.com; Tue, 18 Apr 2006 16:05:37 -0400 (EDT)
7, 24 -- Date: Tue, 18 Apr 2006 16:00:23 -0400
7, 24 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
7, 24 -- Subject: Re: [Microscopy] LM paraffin section methods troubleshoot
7, 24 -- In-reply-to: {200604181933.k3IJX0Vs011688-at-ns.microscopy.com}
7, 24 -- Sender: lcgould-at-med.cornell.edu
7, 24 -- To: kellie.l.garner-at-monsanto.com,
7, 24 -- Microscopy Listserver {microscopy-at-microscopy.com}
7, 24 -- Message-id: {p06230912c06af35bbe9d-at-[140.251.48.23]}
7, 24 -- MIME-version: 1.0
7, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed
7, 24 -- References: {200604181933.k3IJX0Vs011688-at-ns.microscopy.com}
7, 24 -- X-PMX-Version: 5.1.2.240295, Antispam-Engine: 2.3.0.1, Antispam-Data: 2006.4.18.124606
==============================End of - Headers==============================




From: RossLM-at-missouri.edu
Date: Tue, 18 Apr 2006 15:35:36 -0500
Subject: [Microscopy]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

4th Annual Short Course and Workshop on
Computer-Assisted Image Analysis and Measurement

Instructor: Dr. John C. Russ

June 28 -30, 2006

University of Missouri
Columbia, MO

Image processing and analysis are critical components of many fields of science
and engineering. This hands-on course, mixing step-by-step exercises, teaches
the fundamental principles and techniques that are essential to
obtain meaningful
and useful results to solve real world problems. With the small class size and
extended lab times, attendees are encouraged to bring their own images for
individualized instruction. Participants will receive a trial version
of the Fovea Pro
software (a comprehensive package of Photoshop plug-ins) including a complete
manual and all images used in the course, a road map guide to image
analysis, and
CEU credits.

Registration deadline: May 19, 2006 Enrollment limit: 15

For further information and an application form, visit our website:
www.emc.missouri.edu/works.htm Or contact
Lou Ross at (573) 882-4777 or at rosslm-at-missouri.edu

--
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=2227
email: rosslm-at-missouri.edu
http://www.emc.missouri.edu

==============================Original Headers==============================
8, 16 -- From RossLM-at-missouri.edu Tue Apr 18 15:35:36 2006
8, 16 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49])
8, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3IKZZIE031393
8, 16 -- for {microscopy-at-microscopy.com} ; Tue, 18 Apr 2006 15:35:35 -0500
8, 16 -- Received: from um-exproto9.um.umsystem.edu ([207.160.151.148]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830);
8, 16 -- Tue, 18 Apr 2006 15:35:34 -0500
8, 16 -- Received: from [128.206.79.116] ([128.206.78.230]) by um-exproto9.um.umsystem.edu over TLS secured channel with Microsoft SMTPSVC(6.0.3790.1830);
8, 16 -- Tue, 18 Apr 2006 15:35:31 -0500
8, 16 -- Mime-Version: 1.0
8, 16 -- X-Sender: RossLM-at-pop.missouri.edu
8, 16 -- Message-Id: {p05200f5fc06afdecd820-at-[128.206.79.116]}
8, 16 -- Date: Tue, 18 Apr 2006 15:35:35 -0500
8, 16 -- To: microscopy-at-microscopy.com
8, 16 -- From: Lou Ross {RossLM-at-missouri.edu}
8, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
8, 16 -- X-OriginalArrivalTime: 18 Apr 2006 20:35:31.0992 (UTC) FILETIME=[A338C980:01C66327]
==============================End of - Headers==============================




From: hagglundk1-at-nku.edu
Date: Wed, 19 Apr 2006 09:10:46 -0500
Subject: [Microscopy] analySIS contact information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I am having troubles with AnalySIS on my Quanta microscope. I would
love to get in touch with Mike Bode or someone in Support at Olympus SIS
to see if we can get it working again.

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu


==============================Original Headers==============================
3, 23 -- From hagglundk1-at-nku.edu Wed Apr 19 09:10:46 2006
3, 23 -- Received: from mailfe2.nku.edu (mailfe2.nku.edu [192.122.237.68])
3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3JEAjJ0022870
3, 23 -- for {microscopy-at-microscopy.com} ; Wed, 19 Apr 2006 09:10:46 -0500
3, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211);
3, 23 -- Wed, 19 Apr 2006 10:10:44 -0400
3, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0
3, 23 -- Content-class: urn:content-classes:message
3, 23 -- MIME-Version: 1.0
3, 23 -- Content-Type: text/plain;
3, 23 -- charset="us-ascii"
3, 23 -- Subject: analySIS contact information
3, 23 -- Date: Wed, 19 Apr 2006 10:10:43 -0400
3, 23 -- Message-ID: {F01E4499C4EC5842A8AB7198141AC72F3586FB-at-mailfac1.hh.nku.edu}
3, 23 -- X-MS-Has-Attach:
3, 23 -- X-MS-TNEF-Correlator:
3, 23 -- Thread-Topic: analySIS contact information
3, 23 -- Thread-Index: AcZjuwwNke2LtdYBRMu6bpKdwSrSLg==
3, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu}
3, 23 -- To: {microscopy-at-microscopy.com}
3, 23 -- X-OriginalArrivalTime: 19 Apr 2006 14:10:44.0544 (UTC) FILETIME=[0C718C00:01C663BB]
3, 23 -- Content-Transfer-Encoding: 8bit
3, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3JEAjJ0022870
==============================End of - Headers==============================




From: peter.heimann-at-uni-bielefeld.de
Date: Wed, 19 Apr 2006 09:47:52 -0500
Subject: [Microscopy] PCR-Diagnosis of aldehyde-fixed tissue possible ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues,
has anyone ever performed PCR-genotyping/analysis of tissue which was
fixed by formaldehyde- and glutaraldehyde (no contact with osmium)?
How do you treat / digest your fixed tissue sample?
Any advice or tips are welcome!
greetings,
Peter Heimann

====================================================

Dr. Peter Heimann Raum: W7-107 / Tel.: +49-(0)521-106-5628
Universitaet Bielefeld Fax: +49-(0)521-106-5654
"Zellbiologie / Cell Biology" W7-107
33501 Bielefeld, Germany www.uni-bielefeld.de/biologie/cellbio



==============================Original Headers==============================
4, 34 -- From peter.heimann-at-uni-bielefeld.de Wed Apr 19 09:47:51 2006
4, 34 -- Received: from smtp-relay1.uni-bielefeld.de (smtp-relay1.uni-bielefeld.de [129.70.4.97])
4, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3JElp6A001035
4, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2006 09:47:51 -0500
4, 34 -- Received: from pmxchannel-daemon.mail1.hrz.uni-bielefeld.de by
4, 34 -- mail1.hrz.uni-bielefeld.de
4, 34 -- (iPlanet Messaging Server 5.2 HotFix 2.09 (built Nov 18 2005))
4, 34 -- id {0IXZ0000153QHZ-at-mail1.hrz.uni-bielefeld.de} for Microscopy-at-microscopy.com;
4, 34 -- Wed, 19 Apr 2006 16:47:50 +0200 (MEST)
4, 34 -- Received: from [129.70.82.133] ([129.70.82.133]) by mail1.hrz.uni-bielefeld.de
4, 34 -- (iPlanet Messaging Server 5.2 HotFix 2.09 (built Nov 18 2005))
4, 34 -- with ESMTPP id {0IXZ00NWT53Q2G-at-mail1.hrz.uni-bielefeld.de} for
4, 34 -- Microscopy-at-microscopy.com; Wed, 19 Apr 2006 16:47:50 +0200 (MEST)
4, 34 -- Date: Wed, 19 Apr 2006 16:47:51 +0200
4, 34 -- From: PETER HEIMANN {peter.heimann-at-uni-bielefeld.de}
4, 34 -- Subject: PCR-Diagnosis of aldehyde-fixed tissue possible ?
4, 34 -- To: Microscopy-at-microscopy.com
4, 34 -- Reply-to: peter.heimann-at-uni-bielefeld.de
4, 34 -- Message-id: {44464D97.3070503-at-UNI-BIELEFELD.DE}
4, 34 -- Organization: Uni Bielefeld
4, 34 -- MIME-version: 1.0
4, 34 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed
4, 34 -- Content-transfer-encoding: 7BIT
4, 34 -- X-Accept-Language: en-us, en
4, 34 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317)
4, 34 -- X-Spam-Level:
4, 34 -- X-UniBi-Spam-Level: , 7%
4, 34 -- X-UniBi-Spam-Rules: __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0,
4, 34 -- __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0,
4, 34 -- __USER_AGENT 0
4, 34 -- X-UniBi-Deliver: Tag
4, 34 -- X-UniBi-EnvFrom: peter.heimann-at-uni-bielefeld.de
4, 34 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.3.0.1,
4, 34 -- Antispam-Data: 2006.4.19.73110, vscan3.hrz.uni-bielefeld.de
==============================End of - Headers==============================




From: mcauliff-at-umdnj.edu
Date: Wed, 19 Apr 2006 10:28:03 -0500
Subject: [Microscopy] spam on this list?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues:

I am getting more and more microscopy-related spam in the past month
or two, usually advertisements for commercial organizations I have never
dealt with or ever heard of. The latest is "Microanalysis News" from
Bruker AXS Microanalysis. I someone harvesting names from our list or what?

Geoff

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



==============================Original Headers==============================
6, 32 -- From mcauliff-at-umdnj.edu Wed Apr 19 10:28:03 2006
6, 32 -- Received: from zix04.umdnj.edu (zix04.UMDNJ.EDU [130.219.34.127])
6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3JFS3Cn011707
6, 32 -- for {microscopy-at-msa.microscopy.com} ; Wed, 19 Apr 2006 10:28:03 -0500
6, 32 -- Received: from zix04.umdnj.edu (ZixVPM [127.0.0.1])
6, 32 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id E95C24BE59
6, 32 -- for {microscopy-at-msa.microscopy.com} ; Wed, 19 Apr 2006 10:28:02 -0500 (CDT)
6, 32 -- Received: from umdnj.edu (imail.UMDNJ.EDU [130.219.34.129])
6, 32 -- by zix04.umdnj.edu (Proprietary) with ESMTP id C2A484BE32
6, 32 -- for {microscopy-at-msa.microscopy.com} ; Wed, 19 Apr 2006 10:28:01 -0500 (CDT)
6, 32 -- Received: from ([130.219.34.131])
6, 32 -- by imail.umdnj.edu with ESMTP id KP-BRACD.65045910;
6, 32 -- Wed, 19 Apr 2006 11:27:33 -0400
6, 32 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu
6, 32 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004))
6, 32 -- id {0IXZ002016E6HY-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu)
6, 32 -- for microscopy-at-msa.microscopy.com; Wed, 19 Apr 2006 11:27:33 -0400 (EDT)
6, 32 -- Received: from [127.0.0.1] ([10.138.2.123])
6, 32 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21
6, 32 -- 2004)) with ESMTP id {0IXZ00IQM6XWQN-at-Polaris.umdnj.edu} for
6, 32 -- microscopy-at-msa.microscopy.com; Wed, 19 Apr 2006 11:27:33 -0400 (EDT)
6, 32 -- Date: Wed, 19 Apr 2006 11:28:28 -0400
6, 32 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
6, 32 -- Subject: spam on this list?
6, 32 -- To: MicroscopyListserver {microscopy-at-msa.microscopy.com}
6, 32 -- Message-id: {4446571C.9090807-at-umdnj.edu}
6, 32 -- MIME-version: 1.0
6, 32 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1
6, 32 -- Content-transfer-encoding: 7BIT
6, 32 -- X-Accept-Language: en-us, en
6, 32 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2)
6, 32 -- Gecko/20040804 Netscape/7.2 (ax)
==============================End of - Headers==============================




From: r-holdford-at-ti.com
Date: Wed, 19 Apr 2006 11:01:12 -0500
Subject: [Microscopy] Re: spam on this list?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Geoff: I doubt Nestor's email list has been compromised. All the
microscopy-related spam I get is from people who already have my address
for one reason or another. The Bruker AXS people may have acquired your
address when they acquired the PGT business. Or got it from some other
source; it's so hard to keep track these days.

mcauliff-at-umdnj.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Colleagues:
}
} I am getting more and more microscopy-related spam in the past month
} or two, usually advertisements for commercial organizations I have never
} dealt with or ever heard of. The latest is "Microanalysis News" from
} Bruker AXS Microanalysis. I someone harvesting names from our list or what?
}
} Geoff
}
}

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


==============================Original Headers==============================
4, 22 -- From r-holdford-at-ti.com Wed Apr 19 11:01:12 2006
4, 22 -- Received: from reloaded.ext.ti.com (reloaded.ext.ti.com [192.94.94.6])
4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3JG1B2Y029391
4, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Apr 2006 11:01:11 -0500
4, 22 -- Received: from dlep31.itg.ti.com ([157.170.170.35])
4, 22 -- by reloaded.ext.ti.com (8.13.4/8.13.4) with ESMTP id k3JG0vBD006137;
4, 22 -- Wed, 19 Apr 2006 11:01:07 -0500 (CDT)
4, 22 -- Received: from [156.117.194.120] (localhost [127.0.0.1])
4, 22 -- by dlep31.itg.ti.com (8.12.11/8.12.11) with ESMTP id k3JG0vTK015025;
4, 22 -- Wed, 19 Apr 2006 11:00:57 -0500 (CDT)
4, 22 -- Message-ID: {44465EB9.8080107-at-ti.com}
4, 22 -- Date: Wed, 19 Apr 2006 11:00:57 -0500
4, 22 -- From: Becky Holdford {r-holdford-at-ti.com}
4, 22 -- Organization: SC Packaging Development -- FA Development
4, 22 -- User-Agent: Thunderbird 1.5 (Windows/20051201)
4, 22 -- MIME-Version: 1.0
4, 22 -- To: mcauliff-at-umdnj.edu, MSA ListServer {Microscopy-at-MSA.Microscopy.Com}
4, 22 -- Subject: Re: [Microscopy] spam on this list?
4, 22 -- References: {200604191528.k3JFSH3n012038-at-ns.microscopy.com}
4, 22 -- In-Reply-To: {200604191528.k3JFSH3n012038-at-ns.microscopy.com}
4, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 22 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: MCarlyle-at-veeco.com
Date: Wed, 19 Apr 2006 11:22:01 -0500
Subject: [Microscopy] AFM-STM-Seeing at the Nanoscale Conference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

---- Call for Papers Deadline Extended to May 5, 2006 ---

You still have time to submit an abstract for the Seeing at the Nanoscale IV International Conference, July 17-20, 2006, at the University of Pennsylvania, Philadelphia. The Abstract Submission deadline date has been extended to Friday, May 5.

The conference theme is Exploring Nanostructure Imaging, Characterization and Modification Using SPM and Related Techniques.

This event-filled, three-day conference provides an optimum forum for "scientists to speak to scientists" on a wide variety of cutting-edge nanotechnology topics, with technical sessions on:

SESSION 1:
Title: Nanomechanical & Local Property Measurements
Focus: Methods to measure static and dynamic nanoscale mechanical and tribological properties, including nanoindenting, scratching and nanoDMA. The session will also concentrate on molecular models and the understanding of fundamental properties in relation to the above measurement modes.
Chair: Greg Meyers, Dow Chemical Company
Guest Speaker: Ozgur Sahin, Harvard University

SESSION 2:
Title: Visualization I: Biomolecules & Biological Processes
Focus: Techniques to image cells, proteins, lipids, and tissue samples in physiologically relevant environments including high resolution imaging of static samples and visualization of dynamic events to measure inter- and intra-molecular forces.
Chair: Jan Hoh, Johns Hopkins School of Medicine
Guest Speaker: Alexander Malkin, Lawrence Livermore National Labs

SESSION 3:
Title: Visualization II: Materials & Polymer Systems
Focus: Methods to image and manipulate, from single macromolecule and functional self-assemblies to complete materials systems
Chair: Sergei Magonov, Veeco Instruments
Guest Speaker: Dimitri Ivanov, Institut de Chimie des Surfaces et Interfaces, France

SESSION 4:
Title: Measurements of Electrical, Optical, Magnetic & Thermal Properties of Materials at the Nanoscale
Focus: Materials characterization in nanometer and sub-micron scale with emphasis on electrical, optical, magnetic and thermal properties
Chair: Sergei Kalinin, Oak Ridge National Laboratory
Guest Speaker: Louis Brus, Columbia University

SESSION 5:
Title: Instrumentation: New Tools and Techniques for Nanoscience
Focus: Innovative and future developments of SPM tools and techniques, probes and sensors
Chair: Ning Xi, Michigan State University
Guest Speaker: Levent Degertekin, Georgia Tech

Contributed papers will be considered for either oral or poster presentation at the conference unless authors request a poster session. The session chairs will review all abstracts. Final abstracts will be posted on the conference website and printed in the conference program.

If you are interested in submitting an abstract, please see www.veeco.com/nanoconference/call_for_papers.asp for detailed submission guidelines and session-specific information.

We know this will be a very dynamic conference, and we look forward to your participation.



Questions: Contact Marlene Carlyle at mcarlyle-at-veeco.com

Veeco Instruments www.veeco.com/nanoconference


==============================Original Headers==============================
16, 20 -- From MCarlyle-at-veeco.com Wed Apr 19 11:22:01 2006
16, 20 -- Received: from sboexch2.int.veeco.com (SBOEXCH2.veeco.com [68.111.35.167])
16, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3JGM0RQ007701
16, 20 -- for {Microscopy-at-Microscopy.com} ; Wed, 19 Apr 2006 11:22:01 -0500
16, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0
16, 20 -- content-class: urn:content-classes:message
16, 20 -- MIME-Version: 1.0
16, 20 -- Content-Type: text/plain;
16, 20 -- charset="iso-8859-1"
16, 20 -- Subject: AFM-STM-Seeing at the Nanoscale Conference
16, 20 -- Date: Wed, 19 Apr 2006 09:22:00 -0700
16, 20 -- Message-ID: {625BD6DD96DE554DBB0558FFE70E0F42013AE7F3-at-sboexch2.int.veeco.com}
16, 20 -- X-MS-Has-Attach:
16, 20 -- X-MS-TNEF-Correlator:
16, 20 -- Thread-Topic: AFM-STM-Seeing at the Nanoscale Conference
16, 20 -- Thread-Index: AcZjzWKwuPsfUptYTomb1j+IYXg1pw==
16, 20 -- From: "Marlene Carlyle" {MCarlyle-at-veeco.com}
16, 20 -- To: {Microscopy-at-Microscopy.com}
16, 20 -- Content-Transfer-Encoding: 8bit
16, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3JGM0RQ007701
==============================End of - Headers==============================




From: hagglundk1-at-nku.edu
Date: Wed, 19 Apr 2006 11:22:46 -0500
Subject: [Microscopy] AnalySIS Thanks, we have contact

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Thanks for the help. I have already been on the phone with their
service department and we are working on it. Once again, the list saves
the day.

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu



==============================Original Headers==============================
4, 23 -- From hagglundk1-at-nku.edu Wed Apr 19 11:22:45 2006
4, 23 -- Received: from mailfe2.nku.edu (exchange.nku.edu [192.122.237.68])
4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3JGMjgW009370
4, 23 -- for {microscopy-at-microscopy.com} ; Wed, 19 Apr 2006 11:22:45 -0500
4, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211);
4, 23 -- Wed, 19 Apr 2006 12:22:45 -0400
4, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0
4, 23 -- Content-class: urn:content-classes:message
4, 23 -- MIME-Version: 1.0
4, 23 -- Content-Type: text/plain;
4, 23 -- charset="us-ascii"
4, 23 -- Subject: AnalySIS Thanks, we have contact
4, 23 -- Date: Wed, 19 Apr 2006 12:22:44 -0400
4, 23 -- Message-ID: {F01E4499C4EC5842A8AB7198141AC72F3586FE-at-mailfac1.hh.nku.edu}
4, 23 -- X-MS-Has-Attach:
4, 23 -- X-MS-TNEF-Correlator:
4, 23 -- Thread-Topic: AnalySIS Thanks, we have contact
4, 23 -- Thread-Index: AcZjzX1KHnP/r8cpTq2S6xCDkcgK6g==
4, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu}
4, 23 -- To: {microscopy-at-microscopy.com}
4, 23 -- X-OriginalArrivalTime: 19 Apr 2006 16:22:45.0363 (UTC) FILETIME=[7D9E9C30:01C663CD]
4, 23 -- Content-Transfer-Encoding: 8bit
4, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k3JGMjgW009370
==============================End of - Headers==============================




From: jmkrupp-at-ucsc.edu
Date: Wed, 19 Apr 2006 15:05:38 -0500
Subject: [Microscopy] Image database?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings:

Any opinions on databases for image collections? We have both Mac and
PC users, something simple would probably be the best.

Thanks

Jon

--
I'm riding in the MS 150 Crusin' to the Coast bike ride May 13 - 14,
2006 to raise money for the National Multiple Sclerosis Society. You
can go to http://www.msconnection.org and follow the links to make an
ePledge if you wish.


Jonathan Krupp
Microscopy & Imaging Lab
C230 Earth & Marine Science
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-ucsc.edu

==============================Original Headers==============================
7, 21 -- From jmkrupp-at-ucsc.edu Wed Apr 19 15:05:38 2006
7, 21 -- Received: from smtp-prod-mx3.ucsc.edu (smtp-prod-mx3.ucsc.edu [128.114.125.45])
7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k3JK5bH2030839
7, 21 -- for {microscopy-at-microscopy.com} ; Wed, 19 Apr 2006 15:05:38 -0500
7, 21 -- Received: from ucsc.edu (cruzmail-fe1.ucsc.edu [128.114.125.5])
7, 21 -- by smtp-prod-mx3.ucsc.edu (8.13.6/8.13.1) with ESMTP id k3JK5NOa024994
7, 21 -- for {microscopy-at-microscopy.com} ; Wed, 19 Apr 2006 13:05:31 -0700 (PDT)
7, 21 -- Received: from [128.114.25.185] (account jmkrupp-at-ucsc.edu [128.114.25.185] verified)
7, 21 -- by copper.ucsc.edu (CommuniGate Pro SMTP 4.3.7)
7, 21 -- with ESMTPA id 53005984 for microscopy-at-microscopy.com; Wed, 19 Apr 2006 13:05:22 -0700
7, 21 -- Mime-Version: 1.0
7, 21 -- Message-Id: {p06230903c06c47fce1bd-at-[128.114.25.185]}
7, 21 -- Date: Wed, 19 Apr 2006 13:05:21 -0700
7, 21 -- To: microscopy-at-microscopy.com
7, 21 -- From: Jon Krupp {jmkrupp-at-ucsc.edu}
7, 21 -- Subject: Image database?
7, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
7, 21 -- X-UCSC-CATS-Information: This message was scanned by the ITS MailScanner
7, 21 -- X-UCSC-CATS-MailScanner: Found to be clean
7, 21 -- X-UCSC-CATS-MailScanner-SpamCheck:
7, 21 -- X-UCSC-CATS-MailScanner-From: jmkrupp-at-ucsc.edu
==============================End of - Headers==============================




From: mcbelanger6-at-hotmail.com
Date: Wed, 19 Apr 2006 15:18:05 -0500
Subject: [Microscopy] Re: spam on this list?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Geoff,

I also received the spam from Bruker AXS Microanalysis, but at another
email address. I guess it has nothing to do with this list.


Marie-Claude Belanger
Montreal

}
} mcauliff-at-umdnj.edu wrote:
} }
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ----------------------------------------------------------------------------
} }
} } Dear Colleagues:
} }
} } I am getting more and more microscopy-related spam in the past month
} } or two, usually advertisements for commercial organizations I have never
} } dealt with or ever heard of. The latest is "Microanalysis News" from
} } Bruker AXS Microanalysis. I someone harvesting names from our list or
} what?
} }
} } Geoff
} }
} }
}
} --
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Becky Holdford (r-holdford-at-ti.com)
} 972-995-2360
} 972-648-8743 (pager)
} SC Packaging FA Development
} Texas Instruments, Inc.
} Dallas, TX
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
}
}
} ==============================Original
} Headers==============================
} 4, 22 -- From r-holdford-at-ti.com Wed Apr 19 11:01:12 2006
} 4, 22 -- Received: from reloaded.ext.ti.com (reloaded.ext.ti.com
} [192.94.94.6])
} 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} k3JG1B2Y029391
} 4, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Apr 2006 11:01:11
} -0500
} 4, 22 -- Received: from dlep31.itg.ti.com ([157.170.170.35])
} 4, 22 -- by reloaded.ext.ti.com (8.13.4/8.13.4) with ESMTP id
} k3JG0vBD006137;
} 4, 22 -- Wed, 19 Apr 2006 11:01:07 -0500 (CDT)
} 4, 22 -- Received: from [156.117.194.120] (localhost