At 13:16 25/07/2006 -0500, you wrote: Hi Dorota, I have a few things to suggest, possibly not required but this is not obvious from the information you provide.
Although the blocks seem hard the fact that they are sticky indicates that there is a polymerisation issue.
I would firstly check out the catalyst. It is supplied by many manufacturers in a very much different condition than previously and would probably be improved if you were to routinely dry it just prior to use (30mins in a c. 45'C oven). You may also be using a 'bad batch of catalyst. We routinely and regularly make our own GMA (JB4 equivalent) and had to return a recent batch of benzoyle peroxide (catalyst) to Sigma as polymerisation was drastically altered following a recent change in production method (despite assurances that it was a direct replacement for the previous chemical).
Your accelerator may have been contaminated or deteriorated during a lengthy period following initial opening of the bottle. We buy the minimum volume available, and replace all our accelerators on a 6 monthly basis - they definitely deteriotrate on storage and the purchase cost is negligible.
Polymerisation - you don't mention restricting air contact. Despite using vacuum, air contact should be minimised by capping or sealing the molds, or by polymerisation in an inert gas atmosphere (such as nitrogen).
Hope this helps, Alastair
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Alastair McKinnon Histology & EM Facility Manager University of Aberdeen, Institute of Medical Science Foresterhill, Aberdeen, AB25 2ZD tel: +44(0) 1224 552923 (office); +44(0) 1224 555911 (lab) fax: +44(0) 1224 559533; email: a.d.mckinnon-at-abdn.ac.uk www.abdn.ac.uk/ims/h-em
==============================Original Headers============================== 12, 18 -- From a.d.mckinnon-at-abdn.ac.uk Tue Aug 1 03:32:50 2006 12, 18 -- Received: from mailhub3.abdn.ac.uk (mailhub3.abdn.ac.uk [139.133.7.13]) 12, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k718Wosx020502 12, 18 -- for {Microscopy-at-Microscopy.Com} ; Tue, 1 Aug 2006 03:32:50 -0500 12, 18 -- Received: from med-0069.ims.abdn.ac.uk ([139.133.159.90] helo=med-0069.abdn.ac.uk) 12, 18 -- by mailhub3.abdn.ac.uk with esmtp (Exim 4.52) 12, 18 -- id 1G7pgF-0006Ur-0k; Tue, 01 Aug 2006 09:32:47 +0100 12, 18 -- Message-Id: {5.2.1.1.0.20060801090513.01118408-at-mailms.abdn.ac.uk} 12, 18 -- X-Sender: pat081-at-mailms.abdn.ac.uk 12, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 12, 18 -- Date: Tue, 01 Aug 2006 09:32:44 +0100 12, 18 -- To: wadowska-at-upei.ca 12, 18 -- From: Alastair McKinnon {a.d.mckinnon-at-abdn.ac.uk} 12, 18 -- Subject: Re: [Microscopy] JB-4 resin problems 12, 18 -- Cc: Microscopy-at-Microscopy.Com 12, 18 -- In-Reply-To: {200607251816.k6PIGI2o014502-at-ns.microscopy.com} 12, 18 -- Mime-Version: 1.0 12, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
The Faculty of Mathematics and Natural Sciences of the University of Groningen invites applications for a tenure track assistant professorship in Surface Science, with specialisation in scanning tunneling microscopy - for details about the position, requirements and application procedure see http://www.rug.nl/fwn/vacatures/vacaturesfwn/206151.
I would most grateful if you could kindly alert suitable candidates to this oppurtunity.Deadline is September 15th, 2006.
Please kindly note that this kind of position foresees the promotion to associate and further on to full professor if certain criteria are fullfilled.
Thank you very much in advance for your help.
Sincerely yours, Petra Rudolf
Prof. Dr. Petra Rudolf Materials Science Centre University of Groningen Nijenborgh 4 9747 AG Groningen, the Netherlands phone: +(31)50-363 4736 e-mail: P.Rudolf-at-rug.nl web page: http://www.surfacesthinfilms.fmns.rug.nl/
I have been curious for some time now about how the quality/age of a certain batch of G-1 (or its equivalents) affects cross-sectioning properties for TEM samples. The shelf life listed on the small bottles purchased from Gatan indicate 1 year, as well as the larger bottles from epo-tek. Has anyone been able to relate poor adhesion, poor ion-milling, etc. to the age or quality of the epoxy? I know people who have used the same bottles of epo-tek for years with no problems.
Thanks, Leslie
Leslie Krupp (Thompson) IBM Almaden Research 650 Harry Road, K19/D1 San Jose, CA 95120-6099 (408) 927-3856
==============================Original Headers============================== 4, 29 -- From lkrupp-at-us.ibm.com Tue Aug 1 16:05:11 2006 4, 29 -- Received: from e4.ny.us.ibm.com (e4.ny.us.ibm.com [32.97.182.144]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k71L5Avb024845 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Aug 2006 16:05:10 -0500 4, 29 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234]) 4, 29 -- by e4.ny.us.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id k71L56XP011677 4, 29 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL) 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Aug 2006 17:05:07 -0400 4, 29 -- Received: from d01av02.pok.ibm.com (d01av02.pok.ibm.com [9.56.224.216]) 4, 29 -- by d01relay02.pok.ibm.com (8.13.6/NCO/VER7.0) with ESMTP id k71L56cA168446 4, 29 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Aug 2006 17:05:06 -0400 4, 29 -- Received: from d01av02.pok.ibm.com (loopback [127.0.0.1]) 4, 29 -- by d01av02.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id k71L56op020767 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Aug 2006 17:05:06 -0400 4, 29 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) 4, 29 -- by d01av02.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id k71L56SH020745 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Aug 2006 17:05:06 -0400 4, 29 -- To: Microscopy-at-microscopy.com 4, 29 -- Subject: quality/shelf life of G-1 epoxy, epo-tek, etc. 4, 29 -- MIME-Version: 1.0 4, 29 -- X-Mailer: Lotus Notes Release 7.0 HF144 February 01, 2006 4, 29 -- Message-ID: {OF9A56A70D.BC20401E-ON852571BD.00702791-882571BD.0073D168-at-us.ibm.com} 4, 29 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} 4, 29 -- Date: Tue, 1 Aug 2006 14:05:03 -0700 4, 29 -- X-MIMETrack: Serialize by Router on D01ML604/01/M/IBM(Release 7.0.1HF269 | June 22, 2006) at 4, 29 -- 08/01/2006 17:05:06, 4, 29 -- Serialize complete at 08/01/2006 17:05:06 4, 29 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
I have used batches of Epo-Tek 353ND that I purchased for longer than two years. I started to increase the amount of harder (as measured by drops) when it got older. Actually, what I did was decrease the number of resin drops and kept the hardner drops to 1.
Finally I mixed what was left and cured it on a hot plate. Warning, if you do this, use a big enough container to catch the boil out. I destroyed the surface of a good hot plate.
If you store it at room temp and not in direct sunlight, I think that you can go about 2 years. After that replace it. I did not see a fall off in the adhesion capability of the epoxy as it aged.
In a past life, I've used the G1, with the same results. Since I believe that it is the same stuff as the 353-ND, I will not say any more about it.
SBT does not sell the Epo-Tek 353-ND, we refer our customers to Epoxy Technology, Inc whose website is www.epotek.com.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
-------- Original Message -------- } From: lkrupp-at-us.ibm.com } Sent: Tuesday, August 01, 2006 5:09 PM } To: Walck-at-SouthBayTech.com } Subject: [Microscopy] quality/shelf life of G-1 epoxy, epo-tek, etc. } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello listers- } } I have been curious for some time now about how the quality/age of a } certain batch of G-1 (or its equivalents) affects cross-sectioning } properties for TEM samples. The shelf life listed on the small bottles } purchased from Gatan indicate 1 year, as well as the larger bottles from } epo-tek. Has anyone been able to relate poor adhesion, poor ion-milling, } etc. to the age or quality of the epoxy? I know people who have used the } same bottles of epo-tek for years with no problems. } } Thanks, } Leslie } } Leslie Krupp (Thompson) } IBM Almaden Research } 650 Harry Road, K19/D1 } San Jose, CA 95120-6099 } (408) 927-3856 } } ==============================Original Headers============================== } 4, 29 -- From lkrupp-at-us.ibm.com Tue Aug 1 16:05:11 2006 } 4, 29 -- Received: from e4.ny.us.ibm.com (e4.ny.us.ibm.com [32.97.182.144]) } 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k71L5Avb024845 } 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Aug 2006 16:05:10 -0500 } 4, 29 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234]) } 4, 29 -- by e4.ny.us.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id k71L56XP011677 } 4, 29 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL) } 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Aug 2006 17:05:07 -0400 } 4, 29 -- Received: from d01av02.pok.ibm.com (d01av02.pok.ibm.com [9.56.224.216]) } 4, 29 -- by d01relay02.pok.ibm.com (8.13.6/NCO/VER7.0) with ESMTP id k71L56cA168446 } 4, 29 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) } 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Aug 2006 17:05:06 -0400 } 4, 29 -- Received: from d01av02.pok.ibm.com (loopback [127.0.0.1]) } 4, 29 -- by d01av02.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id k71L56op020767 } 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Aug 2006 17:05:06 -0400 } 4, 29 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) } 4, 29 -- by d01av02.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id k71L56SH020745 } 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Aug 2006 17:05:06 -0400 } 4, 29 -- To: Microscopy-at-microscopy.com } 4, 29 -- Subject: quality/shelf life of G-1 epoxy, epo-tek, etc. } 4, 29 -- MIME-Version: 1.0 } 4, 29 -- X-Mailer: Lotus Notes Release 7.0 HF144 February 01, 2006 } 4, 29 -- Message-ID: {OF9A56A70D.BC20401E-ON852571BD.00702791-882571BD.0073D168-at-us.ibm.com} } 4, 29 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} } 4, 29 -- Date: Tue, 1 Aug 2006 14:05:03 -0700 } 4, 29 -- X-MIMETrack: Serialize by Router on D01ML604/01/M/IBM(Release 7.0.1HF269 | June 22, 2006) at } 4, 29 -- 08/01/2006 17:05:06, } 4, 29 -- Serialize complete at 08/01/2006 17:05:06 } 4, 29 -- Content-Type: text/plain; charset="US-ASCII" } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 20 -- From walck-at-southbaytech.com Wed Aug 2 00:24:03 2006 5, 20 -- Received: from smtp14.safesecureweb.com (smtp14.safesecureweb.com [66.241.211.108]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k725O26x011759 5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 2 Aug 2006 00:24:02 -0500 5, 20 -- Received: from mail15.safesecureweb.com (ironhide.ad.safesecureweb.com [192.168.2.180]) 5, 20 -- by smtp14.safesecureweb.com (Spam Firewall) with ESMTP 5, 20 -- id B2B6F15BBCF2; Wed, 2 Aug 2006 01:24:01 -0400 (EDT) 5, 20 -- MIME-Version: 1.0 5, 20 -- Date: Wed, 2 Aug 2006 01:23:40 -0400 5, 20 -- Content-Type: text/plain; 5, 20 -- charset=iso-8859-1 5, 20 -- Subject: re: [Microscopy] quality/shelf life of G-1 epoxy, epo-tek, etc. 5, 20 -- From: Scott Walck {walck-at-southbaytech.com} 5, 20 -- Reply-To: Walck-at-southbaytech.com 5, 20 -- To: {lkrupp-at-us.ibm.com} , {microscopy-at-microscopy.com} 5, 20 -- CC: 5, 20 -- Message-ID: {5b68b38b80844900b11aa30fb3f195ea-at-southbaytech.com} 5, 20 -- X-Virus-Scanned: by Barracuda Spam Firewall at safesecureweb.com 5, 20 -- Content-Transfer-Encoding: 8bit 5, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k725O26x011759 ==============================End of - Headers==============================
I'm new to this group and the industry and am still on a learning curve. I want to learn about the field and need a good place to start. With that in mind, I'm hoping someone can refer me to some good sources of information in the field of confocal and in particular, imaging spectral microscopy.
X-from the technical side, I would like to get some overviews of the technology, the applications the suppliers and the pros and the cons. From the business side I'm looking for any reports on market sizes and where can I find general pricing information to get a sense of what things cost? Thanks in advance! I'm interested in any sources of information (i.e. people, websites, specific magazine issues and other literature).
Thanks in advance!
Alexandre Fong, Optronic Laboratories, Inc.
==============================Original Headers============================== 7, 25 -- From aFong-at-olinet.com Thu Aug 3 12:14:52 2006 7, 25 -- Received: from orlandotelco.net (sun-msg-1.orlandotelco.net [216.54.161.51]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k73HEnnF023720 7, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 3 Aug 2006 12:14:51 -0500 7, 25 -- Received: from VPSalesLT (unverified [64.132.123.34]) 7, 25 -- by orlandotelco.net (SurgeMail 3.7b6) with ESMTP id 13834118 7, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 03 Aug 2006 12:40:58 -0400 7, 25 -- Reply-To: {afong-at-olinet.com} 7, 25 -- From: "Alex Fong" {aFong-at-olinet.com} 7, 25 -- To: {Microscopy-at-Microscopy.Com} 7, 25 -- Subject: LM Need technical and business resources on hyper/multi-spectral imaging microscopy 7, 25 -- Date: Thu, 3 Aug 2006 13:14:49 -0400 7, 25 -- Organization: Optronic Laboratories 7, 25 -- Message-ID: {007801c6b720$536f5700$0c01a8c0-at-VPSalesLT} 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: text/plain; 7, 25 -- charset="us-ascii" 7, 25 -- X-Priority: 3 (Normal) 7, 25 -- X-MSMail-Priority: Normal 7, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 7, 25 -- Importance: Normal 7, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2869 7, 25 -- X-Server: High Performance Mail Server - http://surgemail.com r=809480741 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k73HEnnF023720 ==============================End of - Headers==============================
The Centre for Microscopy and Microanalysis at the University of Queensland (Brisbane, Australia) is seeking a Scientific Officer to work in the Cryo Electron Microscopy facility. The facility is well equipped with a Tecnai F30 (300kV, FEG, GIF), a Tecnai 12 (120kV, LaB6) and a JEOL 1011 TEM, and also a range of ancillary sample preparation equipment (High Pressure Freezers, Vitrobot, etc). The research performed here includes room temperature and cryo tomography, single particle analysis, energy filtered imaging of biological and some materials samples and routine biological TEM imaging.
The full job description can be obtained online at http://www.seek.com.au/users/apply/index.ascx?Sequence=10&PageNumber=1&JobID =7358227&msid=1&Keywords=
The closing date for applications is the 25th of August, 2006. Any questions regarding the position should be directed to me by email (j.riches-at-uq.edu.au).
Regards, Jamie
Jamie Riches Scientific/Technical Manager Centre for Microscopy and Microanalysis University of Queensland Ph: +61 7 3346 2935 Fax: +61 7 3346 2101 Email: j.riches-at-uq.edu.au
==============================Original Headers============================== 14, 22 -- From j.riches-at-uq.edu.au Fri Aug 4 00:04:57 2006 14, 22 -- Received: from imb.uq.edu.au (mail.imb.uq.edu.au [130.102.113.156]) 14, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7454sge013604 14, 22 -- for {microscopy-at-microscopy.com} ; Fri, 4 Aug 2006 00:04:57 -0500 14, 22 -- X-ExtScanner: Niversoft's FindAttachments (free) 14, 22 -- Received: from [130.102.116.81] (HELO AWMJAMIER) 14, 22 -- by imb.uq.edu.au (CommuniGate Pro SMTP 5.0.6) 14, 22 -- with ESMTP id 10616841; Fri, 04 Aug 2006 15:04:52 +1000 14, 22 -- Reply-To: {j.riches-at-uq.edu.au} 14, 22 -- From: "Jamie Riches" {j.riches-at-uq.edu.au} 14, 22 -- To: {microscopy-at-microscopy.com} 14, 22 -- Cc: "'Jill Lumsdale'" {j.lumsdale-at-uq.edu.au} 14, 22 -- Subject: Job Opening: Scientific Officer at Uni. of Queensland, Australia 14, 22 -- Date: Fri, 4 Aug 2006 15:00:02 +1000 14, 22 -- Message-ID: {00f501c6b782$d8201310$51746682-at-AWMJAMIER} 14, 22 -- MIME-Version: 1.0 14, 22 -- Content-Type: text/plain; 14, 22 -- charset="us-ascii" 14, 22 -- Content-Transfer-Encoding: 7bit 14, 22 -- X-Mailer: Microsoft Office Outlook 11 14, 22 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2869 14, 22 -- Thread-Index: Aca3gtf+DOLmfForS3m+MggQiGZosw== ==============================End of - Headers==============================
If anybody out there is using an HRI delay unit with the Picostar image intensifier, we would very much like to speak with you to compare technical details and to seek assistance on troubleshooting.
We'd be happy to communicate by email or to set up a time to call you.
Thank you!! ____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. URL: http://www.aecom.yu.edu/aif/
==============================Original Headers============================== 4, 22 -- From cammer-at-aecom.yu.edu Fri Aug 4 08:11:03 2006 4, 22 -- Received: from mailgw.aecom.yu.edu (mailgw.aecom.yu.edu [129.98.1.16]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k74DB2ul030854 4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 4 Aug 2006 08:11:03 -0500 4, 22 -- Received: from mailvx.aecom.yu.edu (mailvx.aecom.yu.edu [129.98.1.17]) 4, 22 -- by mailgw.aecom.yu.edu (8.12.11.20060308/8.12.11) with SMTP id k74DArGZ014121 4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 4 Aug 2006 09:11:02 -0400 4, 22 -- Received: from post.aecom.yu.edu ([129.98.1.100]) 4, 22 -- by mailvx.aecom.yu.edu (SAVSMTP 3.1.1.32) with SMTP id M2006080409110202270 4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 04 Aug 2006 09:11:02 -0400 4, 22 -- Received: from AIF3.aecom.yu.edu (aif3.aif.aecom.yu.edu [129.98.30.137]) 4, 22 -- by post.aecom.yu.edu (Postfix) with ESMTP id 77A8D25 4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 4 Aug 2006 09:11:02 -0400 (EDT) 4, 22 -- Message-Id: {5.2.1.1.2.20060804091011.011f1a28-at-mailserver.aecom.yu.edu} 4, 22 -- X-Sender: cammer-at-mailserver.aecom.yu.edu 4, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 4, 22 -- Date: Fri, 04 Aug 2006 09:13:06 -0400 4, 22 -- To: microscopy-at-microscopy.com 4, 22 -- From: Michael Cammer {cammer-at-aecom.yu.edu} 4, 22 -- Subject: HRI Picostar, Lavision software question 4, 22 -- Mime-Version: 1.0 4, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both javaidqazi-at-kemet.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: javaidqazi-at-kemet.com Name: javaid qazi
Organization: Kemet Electronics
Title-Subject: [Filtered] 3D stereo microscopy, Height/Roughness profile
Question: I am looking for a commercial lab which can do height profiles by optical/digital stereo microscopy on samples. Basically can give the depth/height of different features on the surface of a part. I know Keyence VHX series can do it, certainly there are other manufacturers as well. Just looking for a place where I can get this done. Preferably on east coast. Thanks a lot.
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Question: Re: Gordon Ante Vrodoljak / microwave fixation tests I am interested in your results with regard to fixation on isolated animal cells without the usual chemical fixatives.
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Email: smythen-at-musc.edu Name: Nancy Smythe
Organization: Medical University of South Carolina
Title-Subject: [Filtered] Processing tissue for light microscopy
Question: I need to process and section whole neonate mice for pariffin. Anyone have a protocol? I am also open for suggestions. I will be fixing over the weekend.
Thanks for your help.
Nancy
Nancy Smythe Department of Otolaryngology Head and Neck Surgery Medical University of South Carolina 843-792-8835 843-792-0368 Fax
Dear SEM techs: My 6100 has an intermittent in which the on-screen characters freeze up, the coarse probe current will not respond, but the focus and magnification controls still operate normally. Pressing the reset button only makes the CRT flicker once but nothing resets--i.e. the magnification and focus don't change, and the on-screen characters stay frozen. Typically, after powering the console off and on, the CRT is covered in random characters, and no image can be formed (i.e. the magnification and focus controls are unresponsive). The problem can disappear for hours at a time, or it can occur almost constantly.
We have tried all manner of reseating PCBs, reconnecting connectors, using freeze spray on the ICs, etc. Nothing affects it in any consistent way.
The Jeol schematics are similar to the actual circuits, but when examined more closely, actual the display circuit boards, for example, are quite different from the schematics, with more than double the number of ICs actually present than shown in the drawings.
Questions: 1. Is there a systematic methodology for isolating the trouble? 2. The local Jeol service office has advised that replacement PCBs are unavailable due to the age of the scope. Are there dealers in used SEM parts? I.e. SEM parts recyclers? 3. Does anyone have a service manual? 4. Are accurate schematics available? 5. Is there such a thing as a replacement console with modern electronics? 6. Is there a list server just for SEM techs?
I have nicely organized and annotated pdf versions of the schematics and the operator's manual that I can offer as a token of my appreciation for any help.
Thanks! Bernard Cuzzillo Berkeley, CA USA
==============================Original Headers============================== 6, 14 -- From bernard-at-bearinc.com Sun Aug 6 12:46:24 2006 6, 14 -- Received: from bearinc.com (bearinc.com [64.62.217.44]) 6, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k76HkNLq022494 6, 14 -- for {Microscopy-at-microscopy.com} ; Sun, 6 Aug 2006 12:46:24 -0500 6, 14 -- Received: from 24.7.101.58 ([24.7.101.58]) by bearinc.com for {Microscopy-at-microscopy.com} ; Sun, 6 Aug 2006 10:46:22 -0700 6, 14 -- Message-ID: {44D62AED.8000406-at-bearinc.com} 6, 14 -- Date: Sun, 06 Aug 2006 10:46:21 -0700 6, 14 -- From: Bernard Cuzzillo {bernard-at-bearinc.com} 6, 14 -- User-Agent: Thunderbird 1.5.0.4 (Windows/20060516) 6, 14 -- MIME-Version: 1.0 6, 14 -- To: Microscopy-at-microscopy.com 6, 14 -- Subject: Jeol JSM-6100 electronics troubleshooting 6, 14 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 14 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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I obviously have a vested interest but why don't you contact JEOL USA?
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==============================Original Headers============================== 4, 17 -- From larry-at-cymru.freewire.co.uk Sun Aug 6 14:22:20 2006 4, 17 -- Received: from get.freewire.net (get.freewire.net [86.54.106.202] (may be forged)) 4, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k76JMJPO001632 4, 17 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sun, 6 Aug 2006 14:22:20 -0500 4, 17 -- Received: from [217.154.254.152] ([217.154.254.152]) 4, 17 -- by get.freewire.net (8.13.6/8.11.6) with ESMTP id k76JMA4s021518; 4, 17 -- Sun, 6 Aug 2006 20:22:13 +0100 4, 17 -- Mime-Version: 1.0 4, 17 -- Message-Id: {p06210200c0fbf14d59bc-at-[217.154.252.73]} 4, 17 -- In-Reply-To: {200608061748.k76Hmr1I024797-at-ns.microscopy.com} 4, 17 -- References: {200608061748.k76Hmr1I024797-at-ns.microscopy.com} 4, 17 -- Date: Sun, 6 Aug 2006 20:21:15 +0100 4, 17 -- To: bernard-at-bearinc.com 4, 17 -- From: Larry Stoter {larry-at-cymru.freewire.co.uk} 4, 17 -- Subject: Re: [Microscopy] Jeol JSM-6100 electronics troubleshooting 4, 17 -- Cc: Microscopy-at-MSA.Microscopy.Com 4, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
If you run out of options try these guys. They do component level repair on almost everything. I had a problem with my Kevex monitor and they fixed it for a flat fee. They also fix all the old crap for the FAA. I have no association with them. I am setting up a JEOL 6300F and am looking for a manual if you come across any.
Thanks!
Tom Kaye
SMH Electronics West Wareham MA 508-291-7447
-----Original Message----- X-from: bernard-at-bearinc.com [mailto:bernard-at-bearinc.com] Sent: Sunday, August 06, 2006 12:54 PM To: tom-at-tomkaye.com
Dear SEM techs: My 6100 has an intermittent in which the on-screen characters freeze up, the coarse probe current will not respond, but the focus and magnification controls still operate normally. Pressing the reset button only makes the CRT flicker once but nothing resets--i.e. the magnification and focus don't change, and the on-screen characters stay frozen. Typically, after powering the console off and on, the CRT is covered in random characters, and no image can be formed (i.e. the magnification and focus controls are unresponsive). The problem can disappear for hours at a time, or it can occur almost constantly.
We have tried all manner of reseating PCBs, reconnecting connectors, using freeze spray on the ICs, etc. Nothing affects it in any consistent way.
The Jeol schematics are similar to the actual circuits, but when examined more closely, actual the display circuit boards, for example, are quite different from the schematics, with more than double the number of ICs actually present than shown in the drawings.
Questions: 1. Is there a systematic methodology for isolating the trouble? 2. The local Jeol service office has advised that replacement PCBs are unavailable due to the age of the scope. Are there dealers in used SEM parts? I.e. SEM parts recyclers? 3. Does anyone have a service manual? 4. Are accurate schematics available? 5. Is there such a thing as a replacement console with modern electronics? 6. Is there a list server just for SEM techs?
I have nicely organized and annotated pdf versions of the schematics and the operator's manual that I can offer as a token of my appreciation for any help.
Thanks! Bernard Cuzzillo Berkeley, CA USA
==============================Original Headers============================== 6, 14 -- From bernard-at-bearinc.com Sun Aug 6 12:46:24 2006 6, 14 -- Received: from bearinc.com (bearinc.com [64.62.217.44]) 6, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k76HkNLq022494 6, 14 -- for {Microscopy-at-microscopy.com} ; Sun, 6 Aug 2006 12:46:24 -0500 6, 14 -- Received: from 24.7.101.58 ([24.7.101.58]) by bearinc.com for {Microscopy-at-microscopy.com} ; Sun, 6 Aug 2006 10:46:22 -0700 6, 14 -- Message-ID: {44D62AED.8000406-at-bearinc.com} 6, 14 -- Date: Sun, 06 Aug 2006 10:46:21 -0700 6, 14 -- From: Bernard Cuzzillo {bernard-at-bearinc.com} 6, 14 -- User-Agent: Thunderbird 1.5.0.4 (Windows/20060516) 6, 14 -- MIME-Version: 1.0 6, 14 -- To: Microscopy-at-microscopy.com 6, 14 -- Subject: Jeol JSM-6100 electronics troubleshooting 6, 14 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 14 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 17, 21 -- From tom-at-tomkaye.com Sun Aug 6 19:09:22 2006 17, 21 -- Received: from tomkaye.com (mxgateway.techpro.com [64.4.207.8]) 17, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7709MbT015147 17, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 6 Aug 2006 19:09:22 -0500 17, 21 -- Received: from TKLAPTOP [70.213.198.83] by tomkaye.com with ESMTP 17, 21 -- (SMTPD32-8.14) id A4ADA410140; Sun, 06 Aug 2006 19:09:17 -0500 17, 21 -- From: "Tom" {tom-at-tomkaye.com} 17, 21 -- To: {Microscopy-at-microscopy.com} 17, 21 -- Subject: RE: [Microscopy] Jeol JSM-6100 electronics troubleshooting 17, 21 -- Date: Sun, 6 Aug 2006 19:09:19 -0500 17, 21 -- Message-ID: {LHECKJAKKIOGKDBHHJMLOEODCKAA.tom-at-tomkaye.com} 17, 21 -- MIME-Version: 1.0 17, 21 -- Content-Type: text/plain; 17, 21 -- charset="iso-8859-1" 17, 21 -- Content-Transfer-Encoding: 7bit 17, 21 -- X-Priority: 3 (Normal) 17, 21 -- X-MSMail-Priority: Normal 17, 21 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0) 17, 21 -- In-reply-to: {200608061753.k76Hro4V030285-at-ns.microscopy.com} 17, 21 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2800.1165 17, 21 -- Importance: Normal ==============================End of - Headers==============================
All, Sorry about that, I guess the way I listed it you could interpret it both ways. Yes that's the company and I don't have any involvement with SMH other than being a customer.
Tom
-----Original Message----- X-from: Ritchie Sims [mailto:r.sims-at-auckland.ac.nz] Sent: Sunday, August 06, 2006 8:28 PM To: tom-at-tomkaye.com
Scott, Leslie; Two years may be OK if you do not examine the specimen. We have repeatedly seen problems with surface reactions that produces contrast of apparent layers along the surface, and also blobs of dark contrast in the epoxy above the surface when it is over six months old. These epoxies cured fine, and did not delaminate or show other signs of reduced strength, but the specimens had to be prepped over again due to the artifact visible in the microscope.
John Mardinly Intel Corporation
The opinion of this author does not necessarily represent the opinion of Intel corporation.
-----Original Message----- X-from: walck-at-southbaytech.com [mailto:walck-at-southbaytech.com] Sent: Tuesday, August 01, 2006 10:24 PM To: Mardinly, John
Leslie,
I have used batches of Epo-Tek 353ND that I purchased for longer than two years. I started to increase the amount of harder (as measured by drops) when it got older. Actually, what I did was decrease the number of resin drops and kept the hardner drops to 1.
Finally I mixed what was left and cured it on a hot plate. Warning, if you do this, use a big enough container to catch the boil out. I destroyed the surface of a good hot plate.
If you store it at room temp and not in direct sunlight, I think that you can go about 2 years. After that replace it. I did not see a fall off in the adhesion capability of the epoxy as it aged.
In a past life, I've used the G1, with the same results. Since I believe that it is the same stuff as the 353-ND, I will not say any more about it.
SBT does not sell the Epo-Tek 353-ND, we refer our customers to Epoxy Technology, Inc whose website is www.epotek.com.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
-------- Original Message -------- } From: lkrupp-at-us.ibm.com } Sent: Tuesday, August 01, 2006 5:09 PM } To: Walck-at-SouthBayTech.com } Subject: [Microscopy] quality/shelf life of G-1 epoxy, epo-tek, etc. } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Hello listers- } } I have been curious for some time now about how the quality/age of a } certain batch of G-1 (or its equivalents) affects cross-sectioning } properties for TEM samples. The shelf life listed on the small bottles } purchased from Gatan indicate 1 year, as well as the larger bottles from } epo-tek. Has anyone been able to relate poor adhesion, poor ion-milling, } etc. to the age or quality of the epoxy? I know people who have used the } same bottles of epo-tek for years with no problems. } } Thanks, } Leslie } } Leslie Krupp (Thompson) } IBM Almaden Research } 650 Harry Road, K19/D1 } San Jose, CA 95120-6099 } (408) 927-3856 } } ==============================Original Headers============================== } 4, 29 -- From lkrupp-at-us.ibm.com Tue Aug 1 16:05:11 2006 } 4, 29 -- Received: from e4.ny.us.ibm.com (e4.ny.us.ibm.com [32.97.182.144]) } 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k71L5Avb024845 } 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Aug 2006 16:05:10 -0500 } 4, 29 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234]) } 4, 29 -- by e4.ny.us.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id k71L56XP011677 } 4, 29 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL) } 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Aug 2006 17:05:07 -0400 } 4, 29 -- Received: from d01av02.pok.ibm.com (d01av02.pok.ibm.com [9.56.224.216]) } 4, 29 -- by d01relay02.pok.ibm.com (8.13.6/NCO/VER7.0) with ESMTP id k71L56cA168446 } 4, 29 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) } 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Aug 2006 17:05:06 -0400 } 4, 29 -- Received: from d01av02.pok.ibm.com (loopback [127.0.0.1]) } 4, 29 -- by d01av02.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id k71L56op020767 } 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Aug 2006 17:05:06 -0400 } 4, 29 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) } 4, 29 -- by d01av02.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id k71L56SH020745 } 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Aug 2006 17:05:06 -0400 } 4, 29 -- To: Microscopy-at-microscopy.com } 4, 29 -- Subject: quality/shelf life of G-1 epoxy, epo-tek, etc. } 4, 29 -- MIME-Version: 1.0 } 4, 29 -- X-Mailer: Lotus Notes Release 7.0 HF144 February 01, 2006 } 4, 29 -- Message-ID: {OF9A56A70D.BC20401E-ON852571BD.00702791-882571BD.0073D168-at-us.ibm.com} } 4, 29 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} } 4, 29 -- Date: Tue, 1 Aug 2006 14:05:03 -0700 } 4, 29 -- X-MIMETrack: Serialize by Router on D01ML604/01/M/IBM(Release 7.0.1HF269 | June 22, 2006) at } 4, 29 -- 08/01/2006 17:05:06, } 4, 29 -- Serialize complete at 08/01/2006 17:05:06 } 4, 29 -- Content-Type: text/plain; charset="US-ASCII" } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 20 -- From walck-at-southbaytech.com Wed Aug 2 00:24:03 2006 5, 20 -- Received: from smtp14.safesecureweb.com (smtp14.safesecureweb.com [66.241.211.108]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k725O26x011759 5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 2 Aug 2006 00:24:02 -0500 5, 20 -- Received: from mail15.safesecureweb.com (ironhide.ad.safesecureweb.com [192.168.2.180]) 5, 20 -- by smtp14.safesecureweb.com (Spam Firewall) with ESMTP 5, 20 -- id B2B6F15BBCF2; Wed, 2 Aug 2006 01:24:01 -0400 (EDT) 5, 20 -- MIME-Version: 1.0 5, 20 -- Date: Wed, 2 Aug 2006 01:23:40 -0400 5, 20 -- Content-Type: text/plain; 5, 20 -- charset=iso-8859-1 5, 20 -- Subject: re: [Microscopy] quality/shelf life of G-1 epoxy, epo-tek, etc. 5, 20 -- From: Scott Walck {walck-at-southbaytech.com} 5, 20 -- Reply-To: Walck-at-southbaytech.com 5, 20 -- To: {lkrupp-at-us.ibm.com} , {microscopy-at-microscopy.com} 5, 20 -- CC: 5, 20 -- Message-ID: {5b68b38b80844900b11aa30fb3f195ea-at-southbaytech.com} 5, 20 -- X-Virus-Scanned: by Barracuda Spam Firewall at safesecureweb.com 5, 20 -- Content-Transfer-Encoding: 8bit 5, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k725O26x011759 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 30 -- From john.mardinly-at-intel.com Sun Aug 6 22:40:08 2006 23, 30 -- Received: from azsmga101-1.ch.intel.com (mga03.intel.com [143.182.124.21]) 23, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k773e8bn005695 23, 30 -- for {Microscopy-at-msa.microscopy.com} ; Sun, 6 Aug 2006 22:40:08 -0500 23, 30 -- Received: from azsmga001.ch.intel.com ([10.2.17.19]) 23, 30 -- by azsmga101-1.ch.intel.com with ESMTP; 06 Aug 2006 20:40:05 -0700 23, 30 -- Received: from scsmsx332.sc.intel.com (HELO scsmsx332.amr.corp.intel.com) ([10.3.90.6]) 23, 30 -- by azsmga001.ch.intel.com with ESMTP; 06 Aug 2006 20:38:47 -0700 23, 30 -- X-IronPort-AV: i="4.07,216,1151910000"; 23, 30 -- d="scan'208"; a="99391439:sNHT143958536988" 23, 30 -- Received: from scsmsx413.amr.corp.intel.com ([10.3.90.32]) by scsmsx332.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.211); 23, 30 -- Sun, 6 Aug 2006 20:38:47 -0700 23, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 23, 30 -- Content-class: urn:content-classes:message 23, 30 -- MIME-Version: 1.0 23, 30 -- Content-Type: text/plain; 23, 30 -- charset="us-ascii" 23, 30 -- Subject: RE: [Microscopy] re: quality/shelf life of G-1 epoxy, epo-tek, etc. 23, 30 -- Date: Sun, 6 Aug 2006 20:38:45 -0700 23, 30 -- Message-ID: {1DF4C4D62339DB4C9C98DF04213995B6B94D79-at-scsmsx413.amr.corp.intel.com} 23, 30 -- X-MS-Has-Attach: 23, 30 -- X-MS-TNEF-Correlator: 23, 30 -- Thread-Topic: [Microscopy] re: quality/shelf life of G-1 epoxy, epo-tek, etc. 23, 30 -- thread-index: Aca18/KSMdelTbaSTcacM7fxAxOYOAD3nDZQ 23, 30 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 23, 30 -- To: {walck-at-southbaytech.com} 23, 30 -- Cc: {Microscopy-at-msa.microscopy.com} 23, 30 -- X-OriginalArrivalTime: 07 Aug 2006 03:38:47.0492 (UTC) FILETIME=[FD8F1040:01C6B9D2] 23, 30 -- Content-Transfer-Encoding: 8bit 23, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k773e8bn005695 ==============================End of - Headers==============================
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Email: polo-at-cameron.net Name: doug
Title-Subject: [Filtered] Acridine Orange and wet mounts
Question: I would be most grateful for any help in formulating an Acridine Orange stain which I can use on blood wet mounts. Any suggestions will be most appreciated. thanks.
I'm a PhD student from the catholic university of Leuven, Belgium and I want to do a TEM on a blend of 2 polymers, filled with particles. The problem is that I have no expercience with this so that's why I send this message. Can somebody help me with my problem? I don't know how I have to prepare my sample. I will give some more information. First I want to put my sample in an oven under N2 for about 7 hours at 220°C. Thereafter, the sample must be cooled down very quickly to freeze the microstructure. After his, I would like to take a TEM picture of the structure. How do I have to prepare my sample to do this? If somebody can explain this in detail, thanks a lot!!
ITEM 1: Tutorial videos from M&M 2006 in Chicago will not be available for about two months. I will post a notice on this list when they are ready for sale.
ITEM 2: At the insistence of MSA leadership, prices for ALL videos will go up on August 15, 2006. Orders recieved prior to that date will be billed at the current price. Orders recieved after that date will be billed at the new prices.
Greg Erdos, MSA Video Wrangler -- Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program Scientific Director, EM Core Lab P.O. Box 118525 University of Florida Gainesville, FL 32611 gwe-at-ufl.edu Phone: 352-392-1295 Fax: 352-846-0251
==============================Original Headers============================== 3, 23 -- From gwe-at-ufl.edu Mon Aug 7 10:44:21 2006 3, 23 -- Received: from smtp.ufl.edu (smtp02.osg.ufl.edu [128.227.74.165]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k77FiIrK018434 3, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 7 Aug 2006 10:44:20 -0500 3, 23 -- Received: from [10.227.60.63] (empc14419.dhcp.clas.ufl.edu [10.227.60.63]) 3, 23 -- (authenticated bits=0) 3, 23 -- by smtp.ufl.edu (8.13.7/8.13.7/2.5.9) with ESMTP id k77FiDI95030110 3, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 7 Aug 2006 11:44:14 -0400 3, 23 -- Message-ID: {44D75FD2.20503-at-ufl.edu} 3, 23 -- Date: Mon, 07 Aug 2006 11:44:18 -0400 3, 23 -- From: Greg Erdos {gwe-at-ufl.edu} 3, 23 -- Reply-To: gwe-at-ufl.edu 3, 23 -- User-Agent: Mozilla Thunderbird 1.0.7 (Windows/20050923) 3, 23 -- X-Accept-Language: en-us, en 3, 23 -- MIME-Version: 1.0 3, 23 -- To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com} 3, 23 -- Subject: Tutorial videos 3, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 23 -- Content-Transfer-Encoding: 7bit 3, 23 -- X-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 3, 23 -- X-UFL-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 3, 23 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 3, 23 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
I'm trying to label the ER of my cell line using ER-Tracker Red (a glibenclamide molecule labelled with BODIP-TR from Molecular Probes). Cells are grown on round coverslips and stained with ER-Tracker and fixed as per the instructions. Unfortunately, I have found that although the ER-Tracker labels the ER it also sticks to the entire coverslip creating a bright red field. Reducing the ER-Tracker concentration reduces the background, but also the ER staining. Blocking with a range of reagents (eg. BSA, FCS, human sera, junk proteins, skim milk etc.) does not seem to reduce this non-specific binding to the coverslip at all.
Does anyone have any advice on how to reduce the background? If so, please let me know.
Thanks in advance, Damien Chong
Molecular Life Sciences Building Gate 8, Victoria Drive The University of Adelaide South Australia 5005
==============================Original Headers============================== 5, 29 -- From damien.chong-at-adelaide.edu.au Mon Aug 7 22:52:21 2006 5, 29 -- Received: from tosh.services.adelaide.edu.au (mailguard-send.adelaide.edu.au [192.43.227.21]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k783qJ4U008836 5, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Aug 2006 22:52:20 -0500 5, 29 -- Received: from stimpy.services.adelaide.edu.au ([10.0.10.10]) 5, 29 -- by tosh.services.adelaide.edu.au with ESMTP; 08 Aug 2006 13:22:18 +0930 5, 29 -- X-IronPort-Anti-Spam-Filtered: true 5, 29 -- X-IronPort-Anti-Spam-Result: AQAAAFyl10QN 5, 29 -- X-IronPort-AV: i="4.07,220,1151850600"; 5, 29 -- d="scan'208"; a="10210367:sNHT15184281" 5, 29 -- Received: from stimpy.services.adelaide.edu.au (localhost.localdomain [127.0.0.1]) 5, 29 -- by stimpy.services.adelaide.edu.au (8.13.1/8.13.1) with ESMTP id k783qI6P010129 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 8 Aug 2006 13:22:18 +0930 5, 29 -- Received: (from apache-at-localhost) 5, 29 -- by stimpy.services.adelaide.edu.au (8.13.1/8.13.1/Submit) id k783qHZb010119 5, 29 -- for Microscopy-at-Microscopy.Com; Tue, 8 Aug 2006 13:22:17 +0930 5, 29 -- Received: from molb00325.molecularbiosciences.adelaide.edu.au (molb00325.molecularbiosciences.adelaide.edu.au [129.127.106.33]) 5, 29 -- by webmail.adelaide.edu.au (IMP) with HTTP 5, 29 -- for {dchong01-at-localhost} ; Tue, 8 Aug 2006 13:22:17 +0930 5, 29 -- Message-ID: {1155009137.44d80a71af3be-at-webmail.adelaide.edu.au} 5, 29 -- Date: Tue, 8 Aug 2006 13:22:17 +0930 5, 29 -- From: Damien Chong {damien.chong-at-adelaide.edu.au} 5, 29 -- To: Microscopy-at-microscopy.com 5, 29 -- Subject: Fluorescence: ER-Tracker Red background 5, 29 -- MIME-Version: 1.0 5, 29 -- Content-Type: text/plain; charset=ISO-8859-1 5, 29 -- Content-Transfer-Encoding: 8bit 5, 29 -- User-Agent: Internet Messaging Program (IMP) 3.2.6 5, 29 -- X-Originating-IP: 129.127.106.33 ==============================End of - Headers==============================
some more info would be useful, e.g. is it a bulk specimen or a film, what kind of polymers are used and what kind of particles (soft/hard) of which size are incorporated?
Best regards,
Petra
--------------------------------------- Dr. Petra Wahlbring Goodyear S.A. Technical Center Analytical Test Laboratories L-7750 Colmar-Berg Luxembourg Tel +352 8199 3725 Fax +352 8199 3905 e-mail: petra.wahlbring-at-goodyear.com
- May Contain Confidential and/or Proprietary Information. May not be copied or disseminated without the expressed written consent of The Goodyear Tire & Rubber Company. -
Steven.Vandebril-at- cit.kuleuven.be To 08/07/06 04:20 PM petra.wahlbring-at-goodyear.com cc
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Hi all,
I'm a PhD student from the catholic university of Leuven, Belgium and I want to do a TEM on a blend of 2 polymers, filled with particles. The problem is that I have no expercience with this so that's why I send this message. Can somebody help me with my problem? I don't know how I have to prepare my sample. I will give some more information. First I want to put my sample in an oven under N2 for about 7 hours at 220°C. Thereafter, the sample must be cooled down very quickly to freeze the microstructure. After his, I would like to take a TEM picture of the structure. How do I have to prepare my sample to do this? If somebody can explain this in detail, thanks a lot!!
I will give some more information about my sample. Tha particles that I use are carbon black particles with a diameter of 20 nm, so TEM is actually the only option to visualize them. The blend is made of PMMA and PaMSAN, a phase separating blend. Thank you for your reactions!
Citeren petra.wahlbring-at-goodyear.com:
} Dear Steve, } } some more info would be useful, e.g. is it a bulk specimen or a film, } what } kind of polymers are used and what kind of particles (soft/hard) of } which } size are incorporated? } } Best regards, } } Petra } } --------------------------------------- } Dr. Petra Wahlbring } Goodyear S.A. Technical Center } Analytical Test Laboratories } L-7750 Colmar-Berg } Luxembourg } Tel +352 8199 3725 } Fax +352 8199 3905 } e-mail: petra.wahlbring-at-goodyear.com } } - May Contain Confidential and/or Proprietary Information. May not } be } copied or disseminated without the expressed written consent of The } Goodyear Tire & Rubber Company. - } } } } } } Steven.Vandebril-at- } } cit.kuleuven.be } } } To } 08/07/06 04:20 PM petra.wahlbring-at-goodyear.com } } } cc } } } Please respond to } Subject } Steven.Vandebril-at- [Microscopy] TEM: sample } } cit.kuleuven.be preparation polymer blend } } } } } } } } } } } } } } } } } } } } } --------------------------------------------------------------------- ------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } --------------------------------------------------------------------- ------- } } } Hi all, } } I'm a PhD student from the catholic university of Leuven, Belgium } and } I want to do a TEM on a blend of 2 polymers, filled with particles. } The problem is that I have no expercience with this so that's why I } send this message. Can somebody help me with my problem? I don't } know } how I have to prepare my sample. I will give some more information. } First I want to put my sample in an oven under N2 for about 7 hours } at } 220°C. Thereafter, the sample must be cooled down very quickly to } freeze the microstructure. After his, I would like to take a TEM } picture of the structure. How do I have to prepare my sample to do } this? If somebody can explain this in detail, thanks a lot!! } } Kind regards, } } Steven } } } Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm } } } ==============================Original } Headers============================== } 4, 30 -- From Steven.Vandebril-at-cit.kuleuven.be Mon Aug 7 09:16:42 } 2006 } 4, 30 -- Received: from thumbler.kulnet.kuleuven.ac.be } (thumbler.kulnet.kuleuven.ac.be [134.58.240.45]) } 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with } ESMTP id k77EGfwc006531 } 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 7 Aug 2006 } 09:16:41 -0500 } 4, 30 -- Received: from localhost (localhost [127.0.0.1]) } 4, 30 -- by thumbler.kulnet.kuleuven.ac.be (Postfix) with } ESMTP } id 44C45138148 } 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 7 Aug 2006 } 16:16:40 +0200 (CEST) } 4, 30 -- Received: from smtp02.kuleuven.be } (lepidus.kulnet.kuleuven.ac.be } [134.58.240.72]) } 4, 30 -- by thumbler.kulnet.kuleuven.ac.be (Postfix) with } ESMTP } id 64D61137E8B } 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 7 Aug 2006 } 16:16:38 +0200 (CEST) } 4, 30 -- Received: from smtp02.kuleuven.be (localhost.localdomain } [127.0.0.1]) } 4, 30 -- by smtp02.kuleuven.be (Postfix) with ESMTP id } 41B822CAA9B; } 4, 30 -- Mon, 7 Aug 2006 16:16:38 +0200 (CEST) } 4, 30 -- Received: from localhost (webmail2.cc.kuleuven.ac.be } [134.58.242.4]) } 4, 30 -- by smtp02.kuleuven.be (Postfix) with ESMTP id } 2E1A52CA9F6 } 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 7 Aug 2006 } 16:16:38 +0200 (CEST) } 4, 30 -- Received: from dhcp194.cit.kuleuven.be } (dhcp194.cit.kuleuven.be } [10.33.63.194]) } 4, 30 -- by webmail2.kuleuven.be (IMP) with HTTP } 4, 30 -- for {u0050286-at-127.0.0.1} ; Mon, 7 Aug 2006 } 16:16:39 } +0200 } 4, 30 -- Message-ID: {1154960199.44d74b4737263-at-webmail2.kuleuven.be} } 4, 30 -- Date: Mon, 7 Aug 2006 16:16:39 +0200 } 4, 30 -- From: Steven Vandebril {Steven.Vandebril-at-cit.kuleuven.be} } 4, 30 -- To: microscopy-at-microscopy.com } 4, 30 -- Subject: TEM: sample preparation polymer blend } 4, 30 -- MIME-Version: 1.0 } 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } 4, 30 -- Content-Transfer-Encoding: 8bit } 4, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2-cvs } 4, 30 -- X-Originating-IP: 10.33.63.194 } 4, 30 -- X-Virus-Scanned: by KULeuven Antivirus Cluster } ==============================End of - } Headers============================== } } }
-- Steven Vandebril Lab of applied rheology and polymer processing Chemical Engineering Department - K.U.Leuven W. de Croylaan 46 3001 Heverlee Tel.:0476 231925 Email:steven.vandebril-at-cit.kuleuven.be
with a soft filler like CB, I think that ultra-microtome cutting of thin sections (at room temperature since Tg's of both polymers are } 100°C) is the method of choice, followed by a Ruthenium Tetroxide staining in order to achieve a contrast between the PaMSAN (stained by RuO4 due to the aromatic ring in the a-methylstyrene) and the PMMA (less stained by RuO4). If you need more detailed information on RuO4 staining, you can contact me again.
With best regards,
Petra
P.S. This report might be of interest for you: http://sunsite.online.globule.org/iupac/publications/pac/1998/pdf/7008x1547.pdf
--------------------------------------- Dr. Petra Wahlbring Goodyear S.A. Technical Center Analytical Test Laboratories L-7750 Colmar-Berg Luxembourg Tel +352 8199 3725 Fax +352 8199 3905 e-mail: petra.wahlbring-at-goodyear.com
- May Contain Confidential and/or Proprietary Information. May not be copied or disseminated without the expressed written consent of The Goodyear Tire & Rubber Company. -
Steven Vandebril {Steven.Vandebril -at-cit.kuleuven.be} To petra.wahlbring-at-goodyear.com 08/08/06 09:08 AM cc Microscopy-at-Microscopy.Com Subject Re: [Microscopy] TEM: sample preparation polymer blend
Hello,
I will give some more information about my sample. Tha particles that I use are carbon black particles with a diameter of 20 nm, so TEM is actually the only option to visualize them. The blend is made of PMMA and PaMSAN, a phase separating blend. Thank you for your reactions!
Citeren petra.wahlbring-at-goodyear.com:
} Dear Steve, } } some more info would be useful, e.g. is it a bulk specimen or a film, } what } kind of polymers are used and what kind of particles (soft/hard) of } which } size are incorporated? } } Best regards, } } Petra } } --------------------------------------- } Dr. Petra Wahlbring } Goodyear S.A. Technical Center } Analytical Test Laboratories } L-7750 Colmar-Berg } Luxembourg } Tel +352 8199 3725 } Fax +352 8199 3905 } e-mail: petra.wahlbring-at-goodyear.com } } - May Contain Confidential and/or Proprietary Information. May not } be } copied or disseminated without the expressed written consent of The } Goodyear Tire & Rubber Company. - } } } } } } Steven.Vandebril-at- } } cit.kuleuven.be } } } To } 08/07/06 04:20 PM petra.wahlbring-at-goodyear.com } } } cc } } } Please respond to } Subject } Steven.Vandebril-at- [Microscopy] TEM: sample } } cit.kuleuven.be preparation polymer blend } } } } } } } } } } } } } } } } } } } } } --------------------------------------------------------------------- ------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } --------------------------------------------------------------------- ------- } } } Hi all, } } I'm a PhD student from the catholic university of Leuven, Belgium } and } I want to do a TEM on a blend of 2 polymers, filled with particles. } The problem is that I have no expercience with this so that's why I } send this message. Can somebody help me with my problem? I don't } know } how I have to prepare my sample. I will give some more information. } First I want to put my sample in an oven under N2 for about 7 hours } at } 220°C. Thereafter, the sample must be cooled down very quickly to } freeze the microstructure. After his, I would like to take a TEM } picture of the structure. How do I have to prepare my sample to do } this? If somebody can explain this in detail, thanks a lot!! } } Kind regards, } } Steven } } } Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm } } } ==============================Original } Headers============================== } 4, 30 -- From Steven.Vandebril-at-cit.kuleuven.be Mon Aug 7 09:16:42 } 2006 } 4, 30 -- Received: from thumbler.kulnet.kuleuven.ac.be } (thumbler.kulnet.kuleuven.ac.be [134.58.240.45]) } 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with } ESMTP id k77EGfwc006531 } 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 7 Aug 2006 } 09:16:41 -0500 } 4, 30 -- Received: from localhost (localhost [127.0.0.1]) } 4, 30 -- by thumbler.kulnet.kuleuven.ac.be (Postfix) with } ESMTP } id 44C45138148 } 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 7 Aug 2006 } 16:16:40 +0200 (CEST) } 4, 30 -- Received: from smtp02.kuleuven.be } (lepidus.kulnet.kuleuven.ac.be } [134.58.240.72]) } 4, 30 -- by thumbler.kulnet.kuleuven.ac.be (Postfix) with } ESMTP } id 64D61137E8B } 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 7 Aug 2006 } 16:16:38 +0200 (CEST) } 4, 30 -- Received: from smtp02.kuleuven.be (localhost.localdomain } [127.0.0.1]) } 4, 30 -- by smtp02.kuleuven.be (Postfix) with ESMTP id } 41B822CAA9B; } 4, 30 -- Mon, 7 Aug 2006 16:16:38 +0200 (CEST) } 4, 30 -- Received: from localhost (webmail2.cc.kuleuven.ac.be } [134.58.242.4]) } 4, 30 -- by smtp02.kuleuven.be (Postfix) with ESMTP id } 2E1A52CA9F6 } 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 7 Aug 2006 } 16:16:38 +0200 (CEST) } 4, 30 -- Received: from dhcp194.cit.kuleuven.be } (dhcp194.cit.kuleuven.be } [10.33.63.194]) } 4, 30 -- by webmail2.kuleuven.be (IMP) with HTTP } 4, 30 -- for {u0050286-at-127.0.0.1} ; Mon, 7 Aug 2006 } 16:16:39 } +0200 } 4, 30 -- Message-ID: {1154960199.44d74b4737263-at-webmail2.kuleuven.be} } 4, 30 -- Date: Mon, 7 Aug 2006 16:16:39 +0200 } 4, 30 -- From: Steven Vandebril {Steven.Vandebril-at-cit.kuleuven.be} } 4, 30 -- To: microscopy-at-microscopy.com } 4, 30 -- Subject: TEM: sample preparation polymer blend } 4, 30 -- MIME-Version: 1.0 } 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } 4, 30 -- Content-Transfer-Encoding: 8bit } 4, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2-cvs } 4, 30 -- X-Originating-IP: 10.33.63.194 } 4, 30 -- X-Virus-Scanned: by KULeuven Antivirus Cluster } ==============================End of - } Headers============================== } } }
-- Steven Vandebril Lab of applied rheology and polymer processing Chemical Engineering Department - K.U.Leuven W. de Croylaan 46 3001 Heverlee Tel.:0476 231925 Email:steven.vandebril-at-cit.kuleuven.be
MANAGER IN CRYOELECTRON MICROSCOPY NEW YORK STRUCTURAL BIOLOGY CENTER
The New York Structural Biology Center seeks a technical manager for a high-end, state or the art facility in Cryoelectron Microscopy. The facility includes four cryoelectron microscopes at 120, 200(2), and 300 kV, the last with a liquid-helium stage and an energy filter, as well as all necessary ancillary equipment. The facility is used by investigators from ten New York academic research institutions on a broad range of biological targets employing all of three CryoEM methodologies: tomography, single-particle analysis, and crystallography. The appointee will work with manufacturers in maintaining optimal performance of the instruments and will work with scientists in pursuing a broad range of biomedical applications, some of which are likely to require new developments. The appointee will supervise an existing team of technical staff with expertise in sample preparation and computational analysis. A strong technical background in electron microscopy is essential including familiarity with biological applications. Compensation will be appropriate and competitive for the candidate's level of experience and training. Send a biographical sketch, a brief statement of previous research experience, together with names and addresses of three individuals who can provide letters of recommendation. Applications should be sent as soon as possible to: David Stokes, at stokes-at-nysbc.org. For more information, see http://www.nysbc.org/facilities/CEM
-- David L. Stokes New York Structural Biology Center tel: 212-939-0660 x116 http://nysbc.org/ fax: 646-219-0300
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wim.vandenbroeck-at-UGent.be as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wim.vandenbroeck-at-UGent.be Name: Wim Van den Broeck
Organization: Department of Morphology, Ghent University
Title-Subject: [Filtered] TEM lab: organization
Question: Dear colleagues,
In the next months, I will have the budget to set up a TEM lab with the following instruments: EM tissue processor, knife maker, ultramicrotome and table, EM trimming instrument, EM staining instrument. In Belgium, as far as I know, Leica is the only company that distributes these instruments. Please, can you tell me if there are other companies, and what the the proís and conís are of the different instruments / different companies. A second question deals with the organization of the lab. I have a room of about 30 m¾ at my disposal with a solid lab table in the centre and two units with fumehoods at one side. Can you give my some tips how to organize the lab as efficiently as possible ?
Thanks in advance for your kind help. Wim Van den Broeck.
I am unable to maintain a high vacuum and the failure message on my Quanta 200 SEM reads that there is a gauge failure and the source is the vacuum. I suspect that it is the Penning gauge and would like to clean it. It is a Pfeiffer model that operates at approximately 2kV. The FEI service rep. refers to this particular gauge as being 'active'. Has anyone had success at dismantling and cleaning this type of gauge. The cost of having it serviced is considerable. Any advice or suggestions on how to proceed with cleaning, etc. can be made to me directly and 'off-line'.
Regards Deb
Debra L. Moreau, Ph.D. Electron Microscopy and Imaging Agriculture and Agri-Food Canada/ Agriculture et Agroalimentaire Canada Telephone/Téléphone: 902 679-6513 Facsimile/Télécopieur: 902 679-2311 32 Main Street Kentville, Nova Scotia B4N 1J5 moreaud-at-agr.gc.ca
==============================Original Headers============================== 6, 26 -- From MoreauD-at-AGR.GC.CA Wed Aug 9 08:33:53 2006 6, 26 -- Received: from agrpazsmtp2.agr.gc.ca (agrpazsmtp2.agr.gc.ca [192.197.71.137]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k79DXrfc005335 6, 26 -- for {Microscopy-at-Microscopy.Com} ; Wed, 9 Aug 2006 08:33:53 -0500 6, 26 -- Received: from onncrxcn4.AGR.GC.CA ([192.168.122.1]) 6, 26 -- by agrpazsmtp2.agr.gc.ca (8.13.6/8.13.3) with ESMTP id k79DUDLs003540 6, 26 -- for {Microscopy-at-Microscopy.Com} ; Wed, 9 Aug 2006 09:30:13 -0400 6, 26 -- Received: from onncrxms3.AGR.GC.CA ([10.117.15.30]) by onncrxcn4.AGR.GC.CA with Microsoft SMTPSVC(5.0.2195.6713); 6, 26 -- Wed, 9 Aug 2006 09:33:34 -0400 6, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 6, 26 -- content-class: urn:content-classes:message 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: text/plain; 6, 26 -- charset="iso-8859-1" 6, 26 -- Subject: Re: cleaning a Penning gauge 6, 26 -- Date: Wed, 9 Aug 2006 09:32:56 -0400 6, 26 -- Message-ID: {E91ED609E45D144C99D56492ABDF74C30352215E-at-onncrxms3.agr.gc.ca} 6, 26 -- X-MS-Has-Attach: 6, 26 -- X-MS-TNEF-Correlator: 6, 26 -- Thread-Topic: Re: cleaning a Penning gauge 6, 26 -- Thread-Index: Aca7uFLDcRLeCq7RT7WggBMv1EaM7A== 6, 26 -- From: "Moreau, Debbie" {MoreauD-at-AGR.GC.CA} 6, 26 -- To: {Microscopy-at-Microscopy.Com} 6, 26 -- X-OriginalArrivalTime: 09 Aug 2006 13:33:34.0340 (UTC) FILETIME=[69676C40:01C6BBB8] 6, 26 -- Content-Transfer-Encoding: 8bit 6, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k79DXrfc005335 ==============================End of - Headers==============================
Dear Wim First, let me congratulate you. In these times of slashed budgets its very good to hear of someone being able to set up a lab, rather than dismantle one! Try to get your hands on a copy of volume 4 of the "Practical Methods in Electron Microscopy" series edited by Audrey M. Glauert: Design of the Electron Microscope Laboratory" by Ronald H. Alderson. I think that its out of print (original copyright was 1975) but it is still an excellent guide and addresses the set up of labs of varying sizes.
Lee Cohen-Gould -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175 http://www.cornellcelldevbiology.org http://www.cornellbiochem.org
==============================Original Headers============================== 2, 24 -- From lcgould-at-med.cornell.edu Wed Aug 9 08:35:36 2006 2, 24 -- Received: from smtp-gw1.med.cornell.edu (smtp-gw1.med.cornell.edu [140.251.3.74]) 2, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k79DZYMU008686 2, 24 -- for {microscopy-at-microscopy.com} ; Wed, 9 Aug 2006 08:35:35 -0500 2, 24 -- Received: from mpx2.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) 2, 24 -- by smtp-gw1.med.cornell.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id k79DZVHA024590 2, 24 -- for {microscopy-at-microscopy.com} ; Wed, 9 Aug 2006 09:35:31 -0400 (EDT) 2, 24 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23]) 2, 24 -- by mpx2.med.cornell.edu 2, 24 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 2, 24 -- with ESMTPA id {0J3Q00GRXGF12240-at-mpx2.med.cornell.edu} for 2, 24 -- microscopy-at-microscopy.com; Wed, 09 Aug 2006 09:35:25 -0400 (EDT) 2, 24 -- Date: Wed, 09 Aug 2006 09:26:41 -0400 2, 24 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 2, 24 -- Subject: Re: [Microscopy] viaWWW: TEM lab: organization 2, 24 -- In-reply-to: {200608091315.k79DF5fv028307-at-ns.microscopy.com} 2, 24 -- Sender: lcgould-at-med.cornell.edu 2, 24 -- To: wim.vandenbroeck-at-UGent.be, 2, 24 -- Microscopy Listserver {microscopy-at-microscopy.com} 2, 24 -- Message-id: {p06230902c0ff91f46e6a-at-[140.251.48.23]} 2, 24 -- MIME-version: 1.0 2, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed 2, 24 -- References: {200608091315.k79DF5fv028307-at-ns.microscopy.com} 2, 24 -- X-PMX-Version: 5.2.0.264296, Antispam-Engine: 2.4.0.264935, Antispam-Data: 2006.8.9.62433 ==============================End of - Headers==============================
Dear All, is there anybody out there who has some layouts for the EDAX 184 pre-amp? I got an EDAX ECON detector which will be attached to an EDAX 9100. I need to know for which pots I need for trimming the pre-amp. There are two pots, one is black, the other gray... I also need a hint where to find the Gain and the Zero pots on the ADC board.
Listers: We would like to upgrade our color imaging system for histological slides - does anybody have any camera systems that they hate/love and could comment on? The Nikon CoolPix seems to be quite popular; how is the software? Can it be convinced to talk to MetaMorph? Has anybody used the MotiCam 2000? It looks spiffy, but I didn't see any references to it in the archives. What about the Orion astronomy camera? We have an old Kodak now that is no longer supported and the software is kind of hopeless, so the users are agitating for an upgrade. We don't want to spend a ton of money, of course...this is just for plain old histo.
Dear Deb, If your Penning gauge is the usual type: cold, with a strong magnet wrapped around it, then you need to take it off the SEM, disassemble it and clean all the bits inside. Whiskers form on the inside over time and these interfere with the ion flow that measures the vacuum. I cannot be more specific because I don't have one of that particular brand. The Edwards ones I have consist of several plates and a needle up the middle. The ion flow is between the needle and plates. The magnet extends the range of the vacuum reading. I just clean the plates and needle and inside of the cylindrical body with fine emery paper and metal polish and wash everything clean with acetone or lab alcohol, dry and re-assemble. Usually works. If you have the instruction manual that comes with the gauge, it may tell you how to clean it, or the SEM maintenance instructions. I hope this helps. Please feel free to contact me if you have any other questions. Regards, Mary Mager Electron Microscopist Department of Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA Tel: 604-822-5648 Fax: 604-822-3619 e-mail: mager-at-interchange.ubc.ca ----- Original Message ----- X-from: {MoreauD-at-AGR.GC.CA} To: {mager-at-interchange.ubc.ca} Sent: Wednesday, August 09, 2006 6:39 AM
Attendance is limited! Additional morning presentations are welcome- please contact John Donovan directly.
EDS Spectrum Imaging and Low Voltage (High Resolution) Analysis, University of Oregon, Eugene, OR
3 day workshop by Dale Newbury (National Institute of Standards & Technology), September, 12-14, 2006
Morning 1 talk: "Silicon Drift Detectors: Energy Dispersive X-ray Spectrometry and X-ray Spectrum Imaging at Output Count Rates Above 100 kHz, and What to Do with All This Data"
Afternoon Demos: Spectral Imaging and Mapping with a conventional EDS (Oxford and Thermo) Bruker Quad SDD and Thermo SDD doing high speed x-ray spectrum image mapping on an FEI Quanta SEM LISPIX hands-on software lab, with pre-recorded x-ray spectrum image data sets.
Morning 2 talk: "Challenges to Successful EDS X-ray Microanalysis in the Low Beam Energy (E0 { 5 keV) Regime"
Afternoon demos: Low Beam Energy SEM/EDS on selected specimens, e.g., SiC, BaTiO3, etc. Demonstration of conventional WDS in the low beam energy regime with BaTiO3 scanning the TiL-O K and BaM spectral regions.
Morning 3 talk: "The Perils of Automatic EDS Analysis: Blunders in Automated Peak Identification of Major, Minor, and Trace Constituents and the Reality of Standardless Analysis"
Afternoon demo: Testing Automatic Peak ID and Standardless Analysis with commercial software in the Laboratory User suggestions for testing (bring your own samples)
Check this page for registration and further details: http://epmalab.uoregon.edu/eds_workshop.htm
Respond to John Donovan (donovan-at-uoregon.edu) to confirm your participation. Registration is required by September 1, 2006. More to come...
John J. Donovan donovan-at-uoregon.edu University of Oregon (541) 346-4632 (office) 1260 Franklin Blvd (541) 346-4655 (probe) Eugene, OR (541) 346-4778 (SEM) 97403-1272 (541) 346-4692 (FAX) Lab Web: http://epmalab.uoregon.edu/
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dirk.durinck-at-mtm.kuleuven.be) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, August 10, 2006 at 04:46:59 ---------------------------------------------------------------------------
I am a PhD student at the Catholic University of Leuven (Belgium) in material sciences with a question about microscopy.
One of my collegues is exploring the capabilities of a CSLM to observe in-situ the high temperature behaviour of refractory materials. The material is not transparent to the laser light, so he can only see the surface of the sample. He is using the cslm because our institute does not have a optical microscope with a heating stage.
A problem he faces is that the image quality deteriorates quite rapidly when the sample reaches high temperatures (1600ƒC). The explanation we are thinking of, is that the sample roughens during the experiment. Measurement has shown that before the tests the roughness was about 0.2µm; after the tests is was 5µm. The small focus plane of the CSLM could thus cause blurring of the image.
In order to elaborate this explanation, we would like to have an idea about the axial (depth, vertical) resolution of the CSLM equipment and other imaging techniques (LOM, SEM). Can someone please suggest a good reference where we can find such data?
Usual failure mode of CCIG gages in SEM and FIB tools is dielectric deposition from hydrocarbon oils and precursor gases on electrodes of the sensor tube, and removal of these deposits fully restores work resource of gage. So far I cleaned hundreds (literally) of Edwards and Varian CCIG sensors by following procedure:
1. Dismount CCIG from the tool, remove magnet, and carefully take sensor tube apart.
2. Soak all the metal parts of the sensor tube overnight in 50/50 solution of Micro 90 and DI or distilled water. Micro 90 is available from Cole Parmer http://www.coleparmer.ca/catalog/product_view.asp?sku=1810001&pfx=EW and if you do not have DI then distilled water from supermarket works. Avoid tap water though.
3. In the morning sonic metal parts for 10 min. in the same solution in which they were soaking. At this point all deposits would be removed from the electrodes and you should see nice shiny metal surfaces.
4. Thoroughly rinse the parts, dry them (hot plate or oven to speed up), and put the sensor tube together - done.
Micro 90 cleaning is very gentle, does not scratch electrodes, and takes minimum of your own time - you can do other things while gage is soaking or drying.
If you do not mind time investment, cleaning by hand-polishing of the electrodes, which was already suggested, will also work.
The only thing I can not help you with is how to take apart Pfeiffer gage; I did cleaning of only Edwards and Varian gages so far.
The best for you would probably be to pre-clean one or two old gages, which you most likely have laying around the lab, and replace gage on the tool as needed.
Cheers, Valery
-----Original Message----- X-from: MoreauD-at-AGR.GC.CA [mailto:MoreauD-at-AGR.GC.CA] Sent: Wednesday, August 09, 2006 9:35 AM To: vray-at-partbeamsystech.com
Good morning.
I am unable to maintain a high vacuum and the failure message on my Quanta 200 SEM reads that there is a gauge failure and the source is the vacuum. I suspect that it is the Penning gauge and would like to clean it. It is a Pfeiffer model that operates at approximately 2kV. The FEI service rep. refers to this particular gauge as being 'active'. Has anyone had success at dismantling and cleaning this type of gauge. The cost of having it serviced is considerable. Any advice or suggestions on how to proceed with cleaning, etc. can be made to me directly and 'off-line'.
Regards Deb
Debra L. Moreau, Ph.D. Electron Microscopy and Imaging Agriculture and Agri-Food Canada/ Agriculture et Agroalimentaire Canada Telephone/Téléphone: 902 679-6513 Facsimile/Télécopieur: 902 679-2311 32 Main Street Kentville, Nova Scotia B4N 1J5 moreaud-at-agr.gc.ca
==============================Original Headers============================== 6, 26 -- From MoreauD-at-AGR.GC.CA Wed Aug 9 08:33:53 2006 6, 26 -- Received: from agrpazsmtp2.agr.gc.ca (agrpazsmtp2.agr.gc.ca [192.197.71.137]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k79DXrfc005335 6, 26 -- for {Microscopy-at-Microscopy.Com} ; Wed, 9 Aug 2006 08:33:53 -0500 6, 26 -- Received: from onncrxcn4.AGR.GC.CA ([192.168.122.1]) 6, 26 -- by agrpazsmtp2.agr.gc.ca (8.13.6/8.13.3) with ESMTP id k79DUDLs003540 6, 26 -- for {Microscopy-at-Microscopy.Com} ; Wed, 9 Aug 2006 09:30:13 -0400 6, 26 -- Received: from onncrxms3.AGR.GC.CA ([10.117.15.30]) by onncrxcn4.AGR.GC.CA with Microsoft SMTPSVC(5.0.2195.6713); 6, 26 -- Wed, 9 Aug 2006 09:33:34 -0400 6, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 6, 26 -- content-class: urn:content-classes:message 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: text/plain; 6, 26 -- charset="iso-8859-1" 6, 26 -- Subject: Re: cleaning a Penning gauge 6, 26 -- Date: Wed, 9 Aug 2006 09:32:56 -0400 6, 26 -- Message-ID: {E91ED609E45D144C99D56492ABDF74C30352215E-at-onncrxms3.agr.gc.ca} 6, 26 -- X-MS-Has-Attach: 6, 26 -- X-MS-TNEF-Correlator: 6, 26 -- Thread-Topic: Re: cleaning a Penning gauge 6, 26 -- Thread-Index: Aca7uFLDcRLeCq7RT7WggBMv1EaM7A== 6, 26 -- From: "Moreau, Debbie" {MoreauD-at-AGR.GC.CA} 6, 26 -- To: {Microscopy-at-Microscopy.Com} 6, 26 -- X-OriginalArrivalTime: 09 Aug 2006 13:33:34.0340 (UTC) FILETIME=[69676C40:01C6BBB8] 6, 26 -- Content-Transfer-Encoding: 8bit 6, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k79DXrfc005335 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 24 -- From vray-at-partbeamsystech.com Thu Aug 10 11:04:20 2006 26, 24 -- Received: from smtp106.plus.mail.re2.yahoo.com (smtp106.plus.mail.re2.yahoo.com [206.190.53.31]) 26, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k7AG4K6i014613 26, 24 -- for {microscopy-at-microscopy.com} ; Thu, 10 Aug 2006 11:04:20 -0500 26, 24 -- Message-Id: {200608101604.k7AG4K6i014613-at-ns.microscopy.com} 26, 24 -- Received: (qmail 64034 invoked from network); 10 Aug 2006 16:04:19 -0000 26, 24 -- Received: from unknown (HELO MEOMobile1) (partbeamsystech-at-200.88.244.199 with login) 26, 24 -- by smtp106.plus.mail.re2.yahoo.com with SMTP; 10 Aug 2006 16:04:19 -0000 26, 24 -- Reply-To: {vray-at-partbeamsystech.com} 26, 24 -- From: "Valery Ray" {vray-at-partbeamsystech.com} 26, 24 -- To: {MoreauD-at-AGR.GC.CA} 26, 24 -- Cc: {microscopy-at-microscopy.com} 26, 24 -- Subject: RE: [Microscopy] Re: cleaning a Penning gauge 26, 24 -- Date: Thu, 10 Aug 2006 12:04:31 -0400 26, 24 -- Organization: PBST / MEO Engineering 26, 24 -- MIME-Version: 1.0 26, 24 -- Content-Type: text/plain; 26, 24 -- charset="iso-8859-1" 26, 24 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 26, 24 -- Thread-Index: Aca7uJOFw7wknLrzSqW8BR6F3OFbbAA2NhNw 26, 24 -- In-Reply-To: {200608091334.k79DYi8Y007350-at-ns.microscopy.com} 26, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2869 26, 24 -- Content-Transfer-Encoding: 8bit 26, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k7AG4K6i014613 ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (cbennett-at-tulane.edu) from on Thursday, August 10, 2006 at 14:45:53 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both cbennett-at-tulane.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Title: cryoSEM of leukemia cell surface (membrane)
Question: I am attempting to visualize the cellular surface of leukemia cells (~10um) after treatment with a peptide using cryoSEM. I am interested in noting the changes in the membrane such as membrane blebbing or the formation of pores. Currently, I am incubating the cells with peptide in RPMI 1640 in the presence of polyK coated slides. After the assay is completed I transfer the slides to fresh RPMI. (I have tried tranferring into water, but the images are of poorer quality - more ice crystals) I perform a 10min sublimation at -95C for the samples. The images that I get vary from day to day and I was wondering if there was a better way to prepare my samples just prior to loading. Thank you.
The University of Missouri is looking for an ASSOCIATE DIRECTOR for a campus-wide light microscopy imaging facility.
Applicants should have experience in some or all of the following areas: * confocal scanning laser microscopy * bright field microscopy * wide field fluorescence microscopy * deconvolution * FRET/FLIM * image processing/analysis * all types of microtomy * immunocytochemistry * in situ hybridization
The Associate Director will be responsible for training users, maintaining instruments and developing protocols for a campus-wide multi-user facility. PhD desirable but not required for individuals with extensive experience. Although an ideal candidate would have experience in all of the areas listed above, candidates with extensive experience in selected areas and who have the desire and capacity to learn the additional areas will be considered. Excellent oral and written communication skills are essential. Experience in a multi-user core facility would be viewed positively. Electron microscopy is not a component of this core facility. Women and minority candidates are especially encouraged to apply. Review of applications will begin immediately and continue until an appropriate candidate is hired. This is a full-time, benefit eligible position. Opportunities for professional development will be encouraged.
The Core's web site can be viewed at www.biotech.missouri.edu/mcc/index.html--|http://www.biotech.missouri.edu/mcc/index.html
Available instrumentation includes: * Zeiss Meta NLO 2 Photon confocal system * BioRad Radiance 2000 confocal system * Leica DMI4000 B and Eppendorf microjection system * Olympus IX70 and Nikon Optiphot 2 widefield systems * Leica stereoscope equipped with epi-fluorescence excitation * Intavis InsituPro VS in situ hybridization robotic system * Autodeblur deconvolution software (Autoquant Inc.). * Metamorph, Imaris Suite, ImagePro image analysis and processing software. * Leica ultramicrotome, cryostat, paraffin microtomes Address applications (CV and 3 letters of reference) or inquires to:
Thomas E. Phillips, Ph.D. Division of Biological Sciences 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) PhillipsT-at-missouri.edu
==============================Original Headers============================== 7, 18 -- From PhillipsT-at-missouri.edu Fri Aug 11 08:59:53 2006 7, 18 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7BDxqY1024585 7, 18 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 11 Aug 2006 08:59:52 -0500 7, 18 -- Received: from um-nsmtpout1.um.umsystem.edu ([209.106.228.53]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 7, 18 -- Fri, 11 Aug 2006 08:59:52 -0500 7, 18 -- Received: from phillips-dell.missouri.edu ([128.206.81.132]) by um-nsmtpout1.um.umsystem.edu over TLS secured channel with Microsoft SMTPSVC(6.0.3790.1830); 7, 18 -- Fri, 11 Aug 2006 08:59:51 -0500 7, 18 -- Message-Id: {6.0.0.22.2.20060811085848.043acb80-at-pop.missouri.edu} 7, 18 -- X-Sender: phillipst-at-pop.missouri.edu 7, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 6.0.0.22 7, 18 -- Date: Fri, 11 Aug 2006 08:59:47 -0500 7, 18 -- To: Microscopy-at-msa.microscopy.com 7, 18 -- From: Tom Phillips {phillipst-at-missouri.edu} 7, 18 -- Subject: Associate Director position LM facility opening 7, 18 -- Mime-Version: 1.0 7, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 18 -- X-OriginalArrivalTime: 11 Aug 2006 13:59:51.0938 (UTC) FILETIME=[6A8D4620:01C6BD4E] ==============================End of - Headers==============================
I have a guy who wants to do diffraction on a protein fibril. We usually look at these things negatively stained with UA and just check for fibrils or no fibrils in the sample. The samples can be kind of variable and junky.
The fibrils are about 12 nm (can this be right?) wide by much longer. I'm not much good with diffraction so I told him I would scout around for someone who could help him.
We are located near the SF bay area. If you could help us with this project, let me know and I will pass along the info.
Thanks
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
==============================Original Headers============================== 8, 21 -- From jmkrupp-at-ucsc.edu Fri Aug 11 15:47:42 2006 8, 21 -- Received: from smtp-prod-mx1.ucsc.edu (smtp-prod-mx1.ucsc.edu [128.114.125.43]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7BKlg12009685 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 11 Aug 2006 15:47:42 -0500 8, 21 -- Received: from ucsc.edu (cruzmail-fe1.ucsc.edu [128.114.125.5]) 8, 21 -- by smtp-prod-mx1.ucsc.edu (8.13.7/8.13.7) with ESMTP id k7BKlOJv001025 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 11 Aug 2006 13:47:36 -0700 (PDT) 8, 21 -- Received: from [128.114.25.186] (account jmkrupp-at-ucsc.edu [128.114.25.186] verified) 8, 21 -- by copper.ucsc.edu (CommuniGate Pro SMTP 4.3.7) 8, 21 -- with ESMTPA id 65895025 for microscopy-at-microscopy.com; Fri, 11 Aug 2006 13:47:29 -0700 8, 21 -- Mime-Version: 1.0 8, 21 -- Message-Id: {p06230903c1029bf13108-at-[128.114.25.186]} 8, 21 -- Date: Fri, 11 Aug 2006 13:47:28 -0700 8, 21 -- To: microscopy-at-microscopy.com 8, 21 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 8, 21 -- Subject: Diffraction on protein fibril? 8, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 8, 21 -- X-UCSC-CATS-Information: This message was scanned by the ITS MailScanner 8, 21 -- X-UCSC-CATS-MailScanner: Found to be clean 8, 21 -- X-UCSC-CATS-MailScanner-SpamCheck: 8, 21 -- X-UCSC-CATS-MailScanner-From: jmkrupp-at-ucsc.edu ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (sawyert-at-science.oregonstate.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, August 11, 2006 at 10:38:32 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both sawyert-at-science.oregonstate.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: I have been working with gfp inserted into plant pathogenic bacterial. My current problem is photo bleaching. The cells have been fixed in para formaldehyde and washed but while trying to capture the image electronically the gfp rapidly bleaches. To quantify data I need an image so any suggestion will be helpful.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (gmerkiso-at-iupui.edu) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, August 12, 2006 at 12:09:54 ---------------------------------------------------------------------------
Email: gmerkiso-at-iupui.edu Name: George Merkison
Organization: Indiana Blood Centter
Education: Undergraduate College
Location: Indianapolis,IN
Question: When using a light microscope, I was taught to keep the condenser down for fluids, including urine sediment and cell counts on hemacytometer, and Nagoette chambers. Is this true and is there a reference to show other individuals? I can't find a reference. Thanks for your reply.
Although I understand the principle behind electron diffraction in TEM I have no idea about the kind of informations this technique gives and how to interpret the diffraction pattern. If you could give me a www address which explains that I would be much grateful.
Now a very practical problem could be perhaps used as an example: we have a mixture of aluminosilicate mineral (I have cut 70 nm sections) with approx. 90% of mordenite and 10% quarz. 1) Can I use e diffraction to distinguish both types of particles? (to verify the purity of the powder) 2) What kind of information about the cristal structure can e diffraction give me in this case? 3) Can I detect a change -and which change- to the cristal structure using this technique if the mineral is heat and treated with strong acids? (which actually modifies the structure)
We have also a tilt stage for tomography. Can this bring further informations?
Regards,
Stephane
__________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com
==============================Original Headers============================== 8, 18 -- From nizets2-at-yahoo.com Mon Aug 14 06:30:17 2006 8, 18 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138]) 8, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k7EBUGao010591 8, 18 -- for {microscopy-at-microscopy.com} ; Mon, 14 Aug 2006 06:30:17 -0500 8, 18 -- Received: (qmail 81606 invoked by uid 60001); 14 Aug 2006 11:30:15 -0000 8, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 8, 18 -- s=s1024; d=yahoo.com; 8, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 8, 18 -- b=rlmt7902ZNRzFTTFfQF7ruMzaWPunDe6Tu9YxDJ2pD9W3I+BsUwz4JFylLSb1NbV5Y1qFqa1jK6YE5BUUAa9EVhASSCrla6W/fKuGhTPt6o7J7paQwTF6sqlqLCbo9YrFe2LwGUS/eGKK8kO3I8ZVczxOMyA1VvnaLJ+c9MHHSw= ; 8, 18 -- Message-ID: {20060814113015.81604.qmail-at-web37406.mail.mud.yahoo.com} 8, 18 -- Received: from [80.122.101.102] by web37406.mail.mud.yahoo.com via HTTP; Mon, 14 Aug 2006 04:30:15 PDT 8, 18 -- Date: Mon, 14 Aug 2006 04:30:15 -0700 (PDT) 8, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} 8, 18 -- Subject: need infos about e diffraction in TEM 8, 18 -- To: microscopy-at-microscopy.com 8, 18 -- MIME-Version: 1.0 8, 18 -- Content-Type: text/plain; charset=iso-8859-1 8, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
The example you give, distinguishing between two phases of different structure in a single sample, is the most common use of electron diffraction. This is essentially a fingerprinting of a structure using a measure of angles and spacings provided by the pattern and fitting these to the geometry of a known unit cell. Strictly speaking, this can only reject a candidate phase by failure to fit, because you aren't really proving the presence of a particular structure, just showing that it's a plausible match to the data. This is nicely suited to your case of only having two structures to choose from.
If you've never done this before, you should try it first on a single-phase material (for example a finely ground silicon). This will give you some practice at orienting a zone axis (you will need to use a double-tilt holder, so this will eliminate the tomography holder unless yours can tilt on two axes). Once you have done this you will also know the camera constant of the microscope for a given camera length, which will provide essential information for distinguishing phases (relates the spacing in A to the distance of a reflection from the pattern origin).
One thing working to your advantage will be the large spacings present in mordenite and the absence of any very large spacings in (alpha) quartz. Because of this, any time you see a reflection indicating a spacing greater than about 4.5 A it must be the mordenite.
Structural changes may be difficult to detect depending on what they are. They would have to involve a fairly large change in the size and shape of the unit cell in order to detect with 'spot pattern' diffraction. If you have a material amenable to convergent beam diffraction you can have a lot more sensitivity to structural changes but your sample must be quite beam stable and have relatively small unit cell (neither is likely to be the case for your mordenite).
As far as references go, the old standard Hirsch et al (Electron Microscopy of Thin Crystals) is pretty good on basic spot pattern indexing - see chapter 5 and appendices 5 and 6 which show some worked examples. In fact, any TEM textbook should have at least some discussion of how to acquire and index spot patterns.
Good Luck, Wharton
************************************************************* Wharton Sinkler, PhD. UOP LLC 50 E. Algonquin Rd. Des Plaines IL 60017-5017 mailto: wharton.sinkler-at-uop.com tel 847-391-3878 fax 847-391-3719
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Monday, August 14, 2006 6:36 AM To: Sinkler, Wharton
Dear listers,
Although I understand the principle behind electron diffraction in TEM I have no idea about the kind of informations this technique gives and how to interpret the diffraction pattern. If you could give me a www address which explains that I would be much grateful.
Now a very practical problem could be perhaps used as an example: we have a mixture of aluminosilicate mineral (I have cut 70 nm sections) with approx. 90% of mordenite and 10% quarz. 1) Can I use e diffraction to distinguish both types of particles? (to verify the purity of the powder) 2) What kind of information about the cristal structure can e diffraction give me in this case? 3) Can I detect a change -and which change- to the cristal structure using this technique if the mineral is heat and treated with strong acids? (which actually modifies the structure)
We have also a tilt stage for tomography. Can this bring further informations?
Regards,
Stephane
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==============================Original Headers============================== 8, 18 -- From nizets2-at-yahoo.com Mon Aug 14 06:30:17 2006 8, 18 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138]) 8, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k7EBUGao010591 8, 18 -- for {microscopy-at-microscopy.com} ; Mon, 14 Aug 2006 06:30:17 -0500 8, 18 -- Received: (qmail 81606 invoked by uid 60001); 14 Aug 2006 11:30:15 -0000 8, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 8, 18 -- s=s1024; d=yahoo.com; 8, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Con tent-Transfer-Encoding; 8, 18 -- b=rlmt7902ZNRzFTTFfQF7ruMzaWPunDe6Tu9YxDJ2pD9W3I+BsUwz4JFylLSb1NbV5Y1qFq a1jK6YE5BUUAa9EVhASSCrla6W/fKuGhTPt6o7J7paQwTF6sqlqLCbo9YrFe2LwGUS/eGKK8 kO3I8ZVczxOMyA1VvnaLJ+c9MHHSw= ; 8, 18 -- Message-ID: {20060814113015.81604.qmail-at-web37406.mail.mud.yahoo.com} 8, 18 -- Received: from [80.122.101.102] by web37406.mail.mud.yahoo.com via HTTP; Mon, 14 Aug 2006 04:30:15 PDT 8, 18 -- Date: Mon, 14 Aug 2006 04:30:15 -0700 (PDT) 8, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} 8, 18 -- Subject: need infos about e diffraction in TEM 8, 18 -- To: microscopy-at-microscopy.com 8, 18 -- MIME-Version: 1.0 8, 18 -- Content-Type: text/plain; charset=iso-8859-1 8, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
==============================Original Headers============================== 23, 26 -- From Wharton.Sinkler-at-uop.com Mon Aug 14 07:37:28 2006 23, 26 -- Received: from AZ18CN849.global.ds.honeywell.com (tmpnat1.honeywell.com [199.64.0.252]) 23, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7ECbPBa021833 23, 26 -- for {microscopy-at-microscopy.com} ; Mon, 14 Aug 2006 07:37:26 -0500 23, 26 -- Received: from dplexch7.uop.com ([138.90.2.112]) by AZ18CN849.global.ds.honeywell.com with Microsoft SMTPSVC(6.0.3790.1830); 23, 26 -- Mon, 14 Aug 2006 05:37:24 -0700 23, 26 -- Received: from DPLEVS1.ad.uop.com ([138.90.2.100]) by dplexch7.uop.com with Microsoft SMTPSVC(6.0.3790.1830); 23, 26 -- Mon, 14 Aug 2006 07:37:23 -0500 23, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 23, 26 -- Content-class: urn:content-classes:message 23, 26 -- MIME-Version: 1.0 23, 26 -- Content-Type: text/plain; 23, 26 -- charset="US-ASCII" 23, 26 -- Subject: RE: [Microscopy] need infos about e diffraction in TEM 23, 26 -- Date: Mon, 14 Aug 2006 07:37:14 -0500 23, 26 -- Message-ID: {62C8A7975D4AE545A23A0CD278BB20A907012FFA-at-DPLEVS1.ad.uop.com} 23, 26 -- X-MS-Has-Attach: 23, 26 -- X-MS-TNEF-Correlator: 23, 26 -- Thread-Topic: [Microscopy] need infos about e diffraction in TEM 23, 26 -- Thread-Index: Aca/ldIkzvC5a3QATaCWBWLUP0Lg8QAA76kw 23, 26 -- From: "Sinkler, Wharton" {Wharton.Sinkler-at-uop.com} 23, 26 -- To: {nizets2-at-yahoo.com} 23, 26 -- Cc: {microscopy-at-microscopy.com} 23, 26 -- X-OriginalArrivalTime: 14 Aug 2006 12:37:23.0010 (UTC) FILETIME=[64000A20:01C6BF9E] 23, 26 -- Content-Transfer-Encoding: 8bit 23, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k7ECbPBa021833 ==============================End of - Headers==============================
I am interested to see if someone else from the list chimes in with an alternitive immulination setting specific for histo/path applications, but as far as I know, in terms of training, experiences, and how I teach my students you always set the illumination (condensor lens) for Köhler illumination. This will result in the best resolution.
Steps:
1) Focus sample
2) stop down the field diaphram until you can see it in through the eyepieces (the one closest to the lamp bulb, may not be present on lower cost scopes like general teaching lab scopes. The aperture closest to the condensrr lens is the stage diaphram or aperture diaphram). Note: If the condensor is extremely far from the sample as you indicate move it very close to the sample.
3) Move the condenor lens up and down to focus on the edges of the field diaphram.
4) Spread the field diaphram out to just inside the field of view through the eyepieces. And center it with the condensor centering knobs.
5) Spread the aperture just outside the filed of view.
---- You are now setup for Köhler illumination on that lens, You wil need to repeat this as you change lenses ----
6) For brightfield: Open the Stage Diaphram all the way. While watching through eyepieces close the stage diaphram until you just see a contrast change and stop (If you remove an eyepiece you will note that the stage diaphram will have cropped 20-30% of the filed of view, this is the 70-80% referred to in some texts)
On 12 Aug 2006, at 12:19, gmerkiso-at-iupui.edu wrote:
} } Email: gmerkiso-at-iupui.edu } Name: George Merkison } } Organization: Indiana Blood Centter } } Education: Undergraduate College } } Location: Indianapolis,IN } } Question: When using a light microscope, I was taught to keep the } condenser down for fluids, including urine sediment and cell counts on } hemacytometer, and Nagoette chambers. Is this true and is there a } reference to show other individuals? I can't find a reference. Thanks } for your reply. } } } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== 8, 12 -- From } zaluzec-at-microscopy.com Sat Aug 12 12:17:44 2006 8, 12 -- Received: } from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 8, 12 -- by } ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } k7CHHgWP017031 8, 12 -- for {microscopy-at-microscopy.com} ; Sat, 12 Aug } 2006 12:17:44 -0500 8, 12 -- Mime-Version: 1.0 8, 12 -- X-Sender: } (Unverified) 8, 12 -- Message-Id: } {p06110411c103bd8f2856-at-[206.69.208.22]} 8, 12 -- Date: Sat, 12 Aug } 2006 12:17:41 -0500 8, 12 -- To: microscopy-at-microscopy.com 8, 12 -- } From: gmerkiso-at-iupui.edu (by way of MicroscopyListserver) 8, 12 -- } Subject: AskAMicroscopist: light microscope & fluids 8, 12 -- } Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"A main-frame: The biggest PC peripheral available."
==============================Original Headers============================== 25, 25 -- From edelmare-at-muohio.edu Mon Aug 14 07:59:55 2006 25, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 25, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7ECxrOR032465 25, 25 -- for {microscopy-at-Microscopy.com} ; Mon, 14 Aug 2006 07:59:54 -0500 25, 25 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 25, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id k7ECxhgs000409; 25, 25 -- Mon, 14 Aug 2006 08:59:43 -0400 25, 25 -- Received: from emf03 ([134.53.14.97]) 25, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id k7ECxgqF031494; 25, 25 -- Mon, 14 Aug 2006 08:59:42 -0400 25, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 25, 25 -- To: gmerkiso-at-iupui.edu, microscopy-at-Microscopy.com 25, 25 -- Date: Mon, 14 Aug 2006 09:00:30 -0400 25, 25 -- MIME-Version: 1.0 25, 25 -- Content-type: text/plain; charset=ISO-8859-1 25, 25 -- Subject: Re: [Microscopy] AskAMicroscopist: light microscope & fluids 25, 25 -- Message-ID: {44E03BAE.18044.DB6F204-at-localhost} 25, 25 -- Priority: normal 25, 25 -- In-reply-to: {200608121719.k7CHJ6Ll018387-at-ns.microscopy.com} 25, 25 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 25, 25 -- X-Real-ConnectIP: 134.53.14.97 25, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 25, 25 -- X-Scanned-By: MIMEDefang 2.52 on 134.53.6.11 25, 25 -- Content-Transfer-Encoding: 8bit 25, 25 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id k7ECxrOR032465 ==============================End of - Headers==============================
Nestor J. Zaluzec wrote: } Colleagues... } } As Local Arrangements Co-Chair for the Microscopy & Microanalysis 2006 Meeting } I would like to take a moment on behalf of the LAC, your local host (MMMS) } and the Co-sponsoring societies (MSA, MAS, IMS, MSC/SMC) } to thank you for attending our meeting in Chicago. } } Attendance at the meeting broke all our records for the last decade with } 1873 Scientific registrants and 3173 total attendee's from 33 countries } discussing 938 presentations. Our exhibit featured over 110 companies presenting } the largest state-of-the-art instrumentation exhibit for microscopy and microanalysis } in the world. } } I hope you found the program stimulating, and the venue and ammenties in Chicagoland } to be both exciting and entertaining. The various committee's and meeting personnel } worked hard to make the conference a success and your participation in the symposia } and scientific exhibition was an essential part of this. } } If you have any suggestions and/or comments on any aspect of the meeting, please } feel free to email them to me (zaluzec-at-aaem.amc.anl.gov) and I will insure they } get collected and passed along to future organizers. } } In reminiscence of the meeting, one of the members of our LAC Team (Byran Rabatic) } has created a s video/slide show which superbly captures the spirit of the } MM2006 meeting . If you have a moment you might enjoy } viewing it. Please feel free to download a copy at the following URL: } } http://mm2006.microscopy.org/MM2006large.mov (~ 115 Mb) } or } http://mm2006.microscopy.org/MM2006small.mov ( ~ 28 Mb) } } make sure you have your audio on when you watch it, it is very well done. } } Again, thanks to all of you for attending MM2006 and we hope to see you } next year in Ft. Lauderdale, Florida (http://mm2007.microscopy.org) . } } Cheers.... } } Nestor Zaluzec } Your Friendly Neighborhood LAC Co-Chair } zaluzec-at-aaem.amc.anl.gov } } } ----------------------------------------------------------------------------------- } It's a 106 miles to Chicago. We got a full tank of gas, half a pack of cigarettes, it's dark and we're wearing sunglasses. } ----------------------------------------------------------------------------------- } } }
-- Gregory W. Erdos, Ph.D. Assistant Director, Biotechnology Program Scientific Director, EM Core Lab P.O. Box 118525 University of Florida Gainesville, FL 32611 gwe-at-ufl.edu Phone: 352-392-1295 Fax: 352-846-0251
==============================Original Headers============================== 3, 26 -- From gwe-at-ufl.edu Mon Aug 14 08:57:08 2006 3, 26 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7EDv5rP011210 3, 26 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 14 Aug 2006 08:57:07 -0500 3, 26 -- Received: from [10.227.60.63] (empc14419.dhcp.clas.ufl.edu [10.227.60.63]) 3, 26 -- (authenticated bits=0) 3, 26 -- by smtp.ufl.edu (8.13.7/8.13.7/2.5.9) with ESMTP id k7EDuuqj426042; 3, 26 -- Mon, 14 Aug 2006 09:56:57 -0400 3, 26 -- Message-ID: {44E08128.9070400-at-ufl.edu} 3, 26 -- Date: Mon, 14 Aug 2006 09:56:56 -0400 3, 26 -- From: Greg Erdos {gwe-at-ufl.edu} 3, 26 -- Reply-To: gwe-at-ufl.edu 3, 26 -- User-Agent: Mozilla Thunderbird 1.0.7 (Windows/20050923) 3, 26 -- X-Accept-Language: en-us, en 3, 26 -- MIME-Version: 1.0 3, 26 -- To: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} , 3, 26 -- "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com} 3, 26 -- Subject: Re: Thanks for Attending MM2006 3, 26 -- References: {p0611040fc103af90e074-at-[206.69.208.22]} 3, 26 -- In-Reply-To: {p0611040fc103af90e074-at-[206.69.208.22]} 3, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 26 -- Content-Transfer-Encoding: 7bit 3, 26 -- X-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 3, 26 -- X-UFL-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 3, 26 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 3, 26 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
Nice summary, but ... The lamp filament should be centered and in focus in the back focal plane of the objective. This can be done by using the Bertrand lens which places an additional lens in the light path. If you don't have one, an ocular can be removed and the back focal plane examined. A centered pin hole in a tube cap or covering of foil can be used to sharpen the image as can a focusing telescope used in phase contrast.
This is done with the specimen in place and in focus. After focusing and centering the filament, you perform the rest. Unfortunately many modern scope have no provision for this operation.
If you have a rotating stage or objectives which can be centered, life gets a little more complex, but the reward of good illumination is worth it!
Frank Karl Degussa Corporation Akron Technical Center 3500 Embassy Parkway Suite 100 Akron, Ohio 44333
330-668-2235 Ext. 238
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edelmare-at-muohio.e du To: frank.karl-at-degussa.com cc: 08/14/2006 09:02 Subject: [Microscopy] Re: AskAMicroscopist: light microscope & fluids AM Please respond to edelmare
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
George:
I am interested to see if someone else from the list chimes in
with an alternitive immulination setting specific for histo/path applications, but as far as I know, in terms of training, experiences, and how I teach my students you always set the illumination (condensor lens) for Köhler illumination. This will result in the best resolution.
Steps:
1) Focus sample
2) stop down the field diaphram until you can see it in through the eyepieces (the one closest to the lamp bulb, may not be present on lower cost scopes like general teaching lab scopes. The aperture closest to the condensrr lens is the stage diaphram or aperture diaphram). Note: If the condensor is extremely far from the sample as you indicate move it very close to the sample.
3) Move the condenor lens up and down to focus on the edges of the field diaphram.
4) Spread the field diaphram out to just inside the field of view through the eyepieces. And center it with the condensor centering knobs.
5) Spread the aperture just outside the filed of view.
---- You are now setup for Köhler illumination on that lens, You wil need to repeat this as you change lenses ----
6) For brightfield: Open the Stage Diaphram all the way. While watching through eyepieces close the stage diaphram until you just see a contrast change and stop (If you remove an eyepiece you will note that the stage diaphram will have cropped 20-30% of the filed of view, this is the 70-80% referred to in some texts)
On 12 Aug 2006, at 12:19, gmerkiso-at-iupui.edu wrote:
} } Email: gmerkiso-at-iupui.edu } Name: George Merkison } } Organization: Indiana Blood Centter } } Education: Undergraduate College } } Location: Indianapolis,IN } } Question: When using a light microscope, I was taught to keep the } condenser down for fluids, including urine sediment and cell counts on } hemacytometer, and Nagoette chambers. Is this true and is there a } reference to show other individuals? I can't find a reference. Thanks } for your reply. } } } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== 8, 12 -- From } zaluzec-at-microscopy.com Sat Aug 12 12:17:44 2006 8, 12 -- Received: } from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 8, 12 -- by } ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } k7CHHgWP017031 8, 12 -- for {microscopy-at-microscopy.com} ; Sat, 12 Aug } 2006 12:17:44 -0500 8, 12 -- Mime-Version: 1.0 8, 12 -- X-Sender: } (Unverified) 8, 12 -- Message-Id: } {p06110411c103bd8f2856-at-[206.69.208.22]} 8, 12 -- Date: Sat, 12 Aug } 2006 12:17:41 -0500 8, 12 -- To: microscopy-at-microscopy.com 8, 12 -- } From: gmerkiso-at-iupui.edu (by way of MicroscopyListserver) 8, 12 -- } Subject: AskAMicroscopist: light microscope & fluids 8, 12 -- } Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"A main-frame: The biggest PC peripheral available."
==============================Original Headers============================== 25, 25 -- From edelmare-at-muohio.edu Mon Aug 14 07:59:55 2006 25, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 25, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7ECxrOR032465 25, 25 -- for {microscopy-at-Microscopy.com} ; Mon, 14 Aug 2006 07:59:54 -0500 25, 25 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 25, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id k7ECxhgs000409; 25, 25 -- Mon, 14 Aug 2006 08:59:43 -0400 25, 25 -- Received: from emf03 ([134.53.14.97]) 25, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id k7ECxgqF031494; 25, 25 -- Mon, 14 Aug 2006 08:59:42 -0400 25, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 25, 25 -- To: gmerkiso-at-iupui.edu, microscopy-at-Microscopy.com 25, 25 -- Date: Mon, 14 Aug 2006 09:00:30 -0400 25, 25 -- MIME-Version: 1.0 25, 25 -- Content-type: text/plain; charset=ISO-8859-1 25, 25 -- Subject: Re: [Microscopy] AskAMicroscopist: light microscope & fluids 25, 25 -- Message-ID: {44E03BAE.18044.DB6F204-at-localhost} 25, 25 -- Priority: normal 25, 25 -- In-reply-to: {200608121719.k7CHJ6Ll018387-at-ns.microscopy.com} 25, 25 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 25, 25 -- X-Real-ConnectIP: 134.53.14.97 25, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 25, 25 -- X-Scanned-By: MIMEDefang 2.52 on 134.53.6.11 25, 25 -- Content-Transfer-Encoding: 8bit 25, 25 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id k7ECxrOR032465 ==============================End of - Headers==============================
==============================Original Headers============================== 51, 20 -- From frank.karl-at-degussa.com Mon Aug 14 09:13:44 2006 51, 20 -- Received: from framailout1.rz.itson.com (mailout2.degussa.com [149.216.91.173]) 51, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7EEDfZB018378 51, 20 -- for {microscopy-at-msa.microscopy.com} ; Mon, 14 Aug 2006 09:13:42 -0500 51, 20 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [172.20.6.74]) 51, 20 -- by framailout1.rz.itson.com (8.13.3/8.13.3/Debian-6) with SMTP id k7EEDRwh014908; 51, 20 -- Mon, 14 Aug 2006 16:13:28 +0200 51, 20 -- In-Reply-To: {200608141302.k7ED25W1002289-at-ns.microscopy.com} 51, 20 -- Subject: Re: [Microscopy] Re: AskAMicroscopist: light microscope & fluids 51, 20 -- To: edelmare-at-muohio.edu, microscopy-at-msa.microscopy.com 51, 20 -- X-Mailer: Lotus Notes Release 6.5.2 June 01, 2004 51, 20 -- Message-ID: {OFF522A4D7.949618D6-ON852571CA.004D2386-852571CA.004E1F47-at-degussa.com} 51, 20 -- From: frank.karl-at-degussa.com 51, 20 -- Date: Mon, 14 Aug 2006 10:13:18 -0400 51, 20 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 51, 20 -- 08/14/2006 09:13:31 AM 51, 20 -- MIME-Version: 1.0 51, 20 -- Content-type: text/plain; charset=ISO-8859-1 51, 20 -- Content-Transfer-Encoding: 8bit 51, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k7EEDfZB018378 ==============================End of - Headers==============================
I tried to send this in html with my comments in red but the server doesn't allow html so:
REGARDING:
Email: gmerkiso-at-iupui.edu Name: George Merkison Organization: Indiana Blood Centter Education: Undergraduate College Location: Indianapolis,IN
Question: When using a light microscope, I was taught to keep the condenser down for fluids, including urine sediment and cell counts on hemacytometer, and Nagoette chambers. Is this true and is there a reference to show other individuals? I can't find a reference. Thanks for your reply.
MY RESPONSE Are you speaking of a flip out condenser or the whole condenser?
1) Flip-out top piece (a separate lens over the main condenser):
If a flip out - it is useful at low mag (10-20X Obj) because it a) lets the light become more parallel to the axis increasing depth of field, and b) increases contrast, and c) allows the main condenser to stay at the appropriate height (condenser height should allow one to close the field diaphram at the base of the scope and have the edges of this iris clearly in view). However, the gain in contrast (which makes things appear to the eye better - enhances refractve index differences between the crystal or biological specimen and the fluid) through the effective lowering of the numerical aperature (cone of light) will decrease resolution of fine details. Generally one flips out the lens because the gross contrast is more important.
2) Whole condenser:
If it is the whole condenser - the answer is no. The condenser should be set at a particular height to properly converge the light upon the specimen in the correct plane. Lowering the condenser does allow the light to become more parallel to the axis as it penetrates the specimen - which then increases contrast, increases depth of field, but it does not do this as well as a flip out lens. It then places the condenser at the wrong height when switching back to do finer detail work. Also, lowering the whole condenser has a tendency to exaggerate speherical and chromatic aberrations in the lens, allow more scattering of light problems, lower overall light intensity and decrease even-ness (sp?) of background lighting. Not recomended. And certainly don't do it if you have a phase contrast scope as the phase ring and annulus aligment will not match in size and may not be axially aligned either.
3) Bottom Line:
If you don't have a flip-out condenser, just use the iris in the condenser to stop down the cone of light to the specimen (this lowers the effective numerical aperature). You will lose resolution, but gain contrast to see objects easier. You can move it back and forth as necessary.
4) As a side note, with the thickness of some hemacytometers and other chambers, you'll probably not be able to get the condenser close enough (high enough) to the specimen for perfection and lowering the condenser just makes it worse.
Tony
Ps After edelmare-at-muohio.edu's message: Most bioscopes do not allow Kohler illumination, they utilize diffuse illumination which is slightly different and attempts to even the light distribution in the image plane by scattering the light at the field diaphram plane (using ground glass or a diffuser over the light bulb). Kohler uses the plane of the light filament itself projected in the plane of the image. A better form of illumination is to remove the diffuser and place a fiberoptic head at the same location as the light bulb (this is what I do sometimes). It provides cool (thus protects filters) pretty even illumination.
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==============================Original Headers============================== 20, 32 -- From ph2-at-sprynet.com Mon Aug 14 09:39:06 2006 20, 32 -- Received: from elasmtp-dupuy.atl.sa.earthlink.net (elasmtp-dupuy.atl.sa.earthlink.net [209.86.89.62]) 20, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7EEd4Gv032317 20, 32 -- for {Microscopy-at-Microscopy.Com} ; Mon, 14 Aug 2006 09:39:05 -0500 20, 32 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 20, 32 -- s=dk20050327; d=sprynet.com; 20, 32 -- b=hEayTEkAYbd/cD2r7K5BkiGgt7S/RLrkO4HmEl7nkQOimU+NIEZb61tXntJhDIl+; 20, 32 -- h=Received:Reply-To:From:To:Subject:Date:Organization:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:X-MSMail-Priority:X-Mailer:Importance:X-MimeOLE:X-ELNK-Trace:X-Originating-IP; 20, 32 -- Received: from [4.224.108.253] (helo=yourw92p4bhlzg) 20, 32 -- by elasmtp-dupuy.atl.sa.earthlink.net with asmtp (TLSv1:RC4-MD5:128) 20, 32 -- (Exim 4.34) 20, 32 -- id 1GCdaj-0007gj-Vo 20, 32 -- for Microscopy-at-Microscopy.Com; Mon, 14 Aug 2006 10:39:00 -0400 20, 32 -- Reply-To: {ph2-at-sprynet.com} 20, 32 -- From: "Tony Havics" {ph2-at-sprynet.com} 20, 32 -- To: "Microscopy Listserve" {Microscopy-at-Microscopy.Com} 20, 32 -- Subject: RE: [Microscopy] AskAMicroscopist: light microscope & fluids 20, 32 -- Date: Mon, 14 Aug 2006 10:47:01 -0500 20, 32 -- Organization: pH2 20, 32 -- Message-ID: {000601c6bfb8$e4617e00$fd6ce004-at-QEPI.local} 20, 32 -- MIME-Version: 1.0 20, 32 -- Content-Type: text/plain; 20, 32 -- charset="utf-8" 20, 32 -- X-Priority: 3 (Normal) 20, 32 -- X-MSMail-Priority: Normal 20, 32 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 20, 32 -- Importance: Normal 20, 32 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2869 20, 32 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f94d0058a98e3ac958cb4740967cf4ba72350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 20, 32 -- X-Originating-IP: 4.224.108.253 20, 32 -- Content-Transfer-Encoding: 8bit 20, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k7EEd4Gv032317 ==============================End of - Headers==============================
"CCD Cameras for Ultra Low Light Microscopy: Technology Overview and Issues to Consider Before Buying."
This Friday (18-August) at 11:30 AM (New York time). See details below.
Admission is free, but connection lines are limited so reserve yours now.
-------------------------------------------------------------- TO RESERVE YOUR CONNECTION LINE -------------------------------------------------------------- Click this URL: https://premconf.webex.com/premconf/j.php?ED=87414217&RG=1
Click REGISTER and complete the requested information. You will be sent a link that gives you access to Friday's meeting.
Details: Cameras utilizing Intensification and Electron-Multiplication technologies are used by life and physical science microscopists to enable the imaging of dynamic events under the most challenging low-light conditions. QImaging is presenting a free, live, interactive, web-based seminar on these two signal amplification technologies on Friday (18-August) at 11:30 AM (New York time).
Who should attend: Anyone interested in learning how these technologies work, what their fundamental limitations are, and what should be considered before acquiring such a camera for their lab should attend. Details are below. There is no cost, but connection lines are limited so reserve yours now.
-------------------------------------------------------------- TO RESERVE YOUR CONNECTION LINE -------------------------------------------------------------- Click this URL: https://premconf.webex.com/premconf/j.php?ED=87414217&RG=1
Click REGISTER and complete the requested information. You will be sent a link that gives you access to Friday's meeting.
---------------------------------------- MEETING SUMMARY ---------------------------------------- Name: "CCD Cameras for Ultra Low Light Microscopy: Technology Overview and Issues to Consider Before Buying."
Hi Everyone, Can anyone recommend a good, easy to use, software package for creating reconstructions from serial TEM sections that will run a PC platform? Would prefer public domain but am open to inexpensive commercial options as well.
Please reply directly and not to the listserve.
Thanks John Shields EM Lab UGA Athens, GA jpshield-at-uga.edu John P. Shields Center for Ultrastructural Research 151 Barrow Hall University of Georgia Athens, GA 30602
706-542-4080
==============================Original Headers============================== 4, 21 -- From jpshield-at-uga.edu Mon Aug 14 14:21:15 2006 4, 21 -- Received: from puntd3.cc.uga.edu (puntd3.cc.uga.edu [128.192.1.109]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7EJLCK8024969 4, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 14 Aug 2006 14:21:14 -0500 4, 21 -- Received: from punts4.cc.uga.edu (punts4.cc.uga.edu [128.192.1.115]) 4, 21 -- by puntd3.cc.uga.edu (MOS 3.7.4b-GA) 4, 21 -- with ESMTP id ABJ51305; 4, 21 -- Mon, 14 Aug 2006 15:21:10 -0400 (EDT) 4, 21 -- Received: (from punts4.cc.uga.edu [128.192.63.198]) 4, 21 -- by punts4.cc.uga.edu (MOS 3.7.4c) 4, 21 -- with HTTPS/1.1 id BCQ04218 (AUTH jpshield-at-uga.edu); 4, 21 -- Mon, 14 Aug 2006 15:20:29 -0400 (EDT) 4, 21 -- From: John Shields {jpshield-at-uga.edu} 4, 21 -- Subject: TEM serial section 3D 4, 21 -- To: msa listserve {Microscopy-at-MSA.Microscopy.Com} 4, 21 -- X-Mailer: Mirapoint Webmail Direct 3.7.4c 4, 21 -- MIME-Version: 1.0 4, 21 -- Content-Type: text/plain; charset=us-ascii 4, 21 -- Content-Transfer-Encoding: 7bit 4, 21 -- Message-Id: {20060814152029.BCQ04218-at-punts4.cc.uga.edu} 4, 21 -- Date: Mon, 14 Aug 2006 15:20:29 -0400 (EDT) ==============================End of - Headers==============================
Anyone have a working LKB Bromma 2208 multiplate wax heater/hot plate warmer for donation or (cheap) sale? Thanks.
Phil -- Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462
==============================Original Headers============================== 3, 20 -- From oshel1pe-at-cmich.edu Mon Aug 14 14:33:51 2006 3, 20 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7EJXnLC029006 3, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Aug 2006 14:33:50 -0500 3, 20 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 3, 20 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id k7EK7IqG006280 3, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Aug 2006 16:07:18 -0400 3, 20 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 3, 20 -- Mon, 14 Aug 2006 15:33:44 -0400 3, 20 -- Mime-Version: 1.0 3, 20 -- Message-Id: {f06230917c106804d8e23-at-[141.209.160.249]} 3, 20 -- Date: Mon, 14 Aug 2006 15:33:41 -0400 3, 20 -- To: Microscopy-at-microscopy.com 3, 20 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 20 -- Subject: LKB 2208 wax heater 3, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 20 -- X-OriginalArrivalTime: 14 Aug 2006 19:33:44.0946 (UTC) FILETIME=[8E64F520:01C6BFD8] 3, 20 -- X-CanItPRO-Stream: default 3, 20 -- X-Spam-Score: -4 () L_EXCH_MF 3, 20 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
Dear Listserver: We have a very small electron microscopy laboratory with 1970;s state of the art equipment (Philips 201 and ETEC Autoscan). I am concerned about the dwindling shortage and increased costs of film, chemistry and paper. I would like to know if either or both of these instruments could be easily converted to digial from film recording. I have not kept up with the ccd camera prices and someone told me they wondered if a good digital camera with an adapter would be available. What kind of reasonable options are there for the old but very functional TEM and SEM? Thanks for your advice. Barbara Plowman
Barbara L. Plowman University of the Pacific Arthur A. Dugoni School of Dentistry 2155 Webster St. Rm 636B San Francisco, CA 94115 ph: 415-929-6692 email: Bplowman-at-pacific.edu
==============================Original Headers============================== 3, 16 -- From Bplowman-at-Pacific.edu Mon Aug 14 14:55:18 2006 3, 16 -- Received: from sfmail.dental.pacific.edu (sfmail.dental.uop.edu [138.9.130.8]) 3, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7EJtG5I013380 3, 16 -- for {Microscopy-at-microscopy.org} ; Mon, 14 Aug 2006 14:55:17 -0500 3, 16 -- Received: from SFUOP-MTA by sfmail.dental.pacific.edu 3, 16 -- with Novell_GroupWise; Mon, 14 Aug 2006 12:55:12 -0700 3, 16 -- Message-Id: {44E072A602000049000042E7-at-sfmail.dental.pacific.edu} 3, 16 -- X-Mailer: Novell GroupWise Internet Agent 7.0 3, 16 -- Date: Mon, 14 Aug 2006 12:55:02 -0700 3, 16 -- From: "Barbara Plowman" {Bplowman-at-Pacific.edu} 3, 16 -- To: {Microscopy-at-microscopy.org} 3, 16 -- Subject: Converting to digital 3, 16 -- Mime-Version: 1.0 3, 16 -- Content-Type: text/plain; charset=US-ASCII 3, 16 -- Content-Transfer-Encoding: 7bit 3, 16 -- Content-Disposition: inline ==============================End of - Headers==============================
Hi We do hundreds of 3D reconstruction from TEM. Just to make a stack of images I use QuickTime. That way people can 'scrub' through the data to see the TEMs. There is no package available (that I know of) that will take raw TEM data and output a 3D rendering that is useful. I use Volocity (not public domain, nor cheep) to do the 3D reconstructions and analytical work. Unless others have ideas, I think you will have to be doing some work with the raw data before the 3D work can be done. Feel free to contact me in you have any questions. If you are only doing a small number, I could do it for you. David
_____________________
David Elliott Ph.D. Assistant Professor Department of Cell Biology and Anatomy University of Arizona College of Medicine PO Box 245004 Tucson, AZ 85724
Voice: 520-626-7870 Fax: 520-626-2097
On Aug 14, 2006, at 12:37 PM, jpshield-at-uga.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi Everyone, } Can anyone recommend a good, easy to use, software package for } creating reconstructions from serial TEM sections that will run a } PC platform? } Would prefer public domain but am open to inexpensive commercial } options as well. } } Please reply directly and not to the listserve. } } Thanks } John Shields } EM Lab } UGA Athens, GA } jpshield-at-uga.edu } John P. Shields } Center for Ultrastructural Research } 151 Barrow Hall } University of Georgia } Athens, GA 30602 } } 706-542-4080 } } ==============================Original } Headers============================== } 4, 21 -- From jpshield-at-uga.edu Mon Aug 14 14:21:15 2006 } 4, 21 -- Received: from puntd3.cc.uga.edu (puntd3.cc.uga.edu } [128.192.1.109]) } 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id k7EJLCK8024969 } 4, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 14 Aug 2006 } 14:21:14 -0500 } 4, 21 -- Received: from punts4.cc.uga.edu (punts4.cc.uga.edu } [128.192.1.115]) } 4, 21 -- by puntd3.cc.uga.edu (MOS 3.7.4b-GA) } 4, 21 -- with ESMTP id ABJ51305; } 4, 21 -- Mon, 14 Aug 2006 15:21:10 -0400 (EDT) } 4, 21 -- Received: (from punts4.cc.uga.edu [128.192.63.198]) } 4, 21 -- by punts4.cc.uga.edu (MOS 3.7.4c) } 4, 21 -- with HTTPS/1.1 id BCQ04218 (AUTH jpshield-at-uga.edu); } 4, 21 -- Mon, 14 Aug 2006 15:20:29 -0400 (EDT) } 4, 21 -- From: John Shields {jpshield-at-uga.edu} } 4, 21 -- Subject: TEM serial section 3D } 4, 21 -- To: msa listserve {Microscopy-at-MSA.Microscopy.Com} } 4, 21 -- X-Mailer: Mirapoint Webmail Direct 3.7.4c } 4, 21 -- MIME-Version: 1.0 } 4, 21 -- Content-Type: text/plain; charset=us-ascii } 4, 21 -- Content-Transfer-Encoding: 7bit } 4, 21 -- Message-Id: {20060814152029.BCQ04218-at-punts4.cc.uga.edu} } 4, 21 -- Date: Mon, 14 Aug 2006 15:20:29 -0400 (EDT) } ==============================End of - } Headers==============================
==============================Original Headers============================== 10, 22 -- From Elliott-at-arizona.edu Mon Aug 14 15:03:35 2006 10, 22 -- Received: from smtpgate.email.arizona.edu (deagol.email.Arizona.EDU [128.196.133.142]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7EK3Xi0022963 10, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 14 Aug 2006 15:03:34 -0500 10, 22 -- Received: from localhost (gimli.email.arizona.edu [10.0.0.223]) 10, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 5B24BF1986A 10, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 14 Aug 2006 13:03:27 -0700 (MST) 10, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 10, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 9E47FF1888E 10, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 14 Aug 2006 12:55:15 -0700 (MST) 10, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 10, 22 -- In-Reply-To: {200608141937.k7EJbJ9U003496-at-ns.microscopy.com} 10, 22 -- References: {200608141937.k7EJbJ9U003496-at-ns.microscopy.com} 10, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 10, 22 -- Message-Id: {39C9C0E8-E1B9-4E04-BD57-06538D9199BF-at-arizona.edu} 10, 22 -- Content-Transfer-Encoding: 7bit 10, 22 -- From: David Elliott {Elliott-at-arizona.edu} 10, 22 -- Subject: Re: [Microscopy] TEM serial section 3D 10, 22 -- Date: Mon, 14 Aug 2006 12:55:14 -0700 10, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 10, 22 -- X-Mailer: Apple Mail (2.752.2) 10, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Dear Listserver: We have a very small electron microscopy laboratory with 1970;s state of the art equipment (Philips 201 and ETEC Autoscan). I am concerned about the dwindling shortage and increased costs of film, chemistry and paper. I would like to know if either or both of these instruments could be easily converted to digial from film recording. I have not kept up with the ccd camera prices and someone told me they wondered if a good digital camera with an adapter would be available. What kind of reasonable options are there for the old but very functional TEM and SEM? Thanks for your advice. Barbara Plowman
Barbara L. Plowman University of the Pacific Arthur A. Dugoni School of Dentistry 2155 Webster St. Rm 636B San Francisco, CA 94115 ph: 415-929-6692 email: Bplowman-at-pacific.edu
==============================Original Headers============================== 3, 16 -- From Bplowman-at-Pacific.edu Mon Aug 14 15:14:40 2006 3, 16 -- Received: from sfmail.dental.pacific.edu (sfmail.dental.uop.edu [138.9.130.8]) 3, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7EKEcV2001664 3, 16 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Aug 2006 15:14:39 -0500 3, 16 -- Received: from SFUOP-MTA by sfmail.dental.pacific.edu 3, 16 -- with Novell_GroupWise; Mon, 14 Aug 2006 13:14:37 -0700 3, 16 -- Message-Id: {44E0773302000049000042F2-at-sfmail.dental.pacific.edu} 3, 16 -- X-Mailer: Novell GroupWise Internet Agent 7.0 3, 16 -- Date: Mon, 14 Aug 2006 13:14:27 -0700 3, 16 -- From: "Barbara Plowman" {Bplowman-at-Pacific.edu} 3, 16 -- To: {Microscopy-at-microscopy.com} 3, 16 -- Subject: Converting to digital 3, 16 -- Mime-Version: 1.0 3, 16 -- Content-Type: text/plain; charset=US-ASCII 3, 16 -- Content-Transfer-Encoding: 7bit 3, 16 -- Content-Disposition: inline ==============================End of - Headers==============================
We prepare thin sections of epoxy embedded samples and came across an LKB 2188 microtome. We currently cut these with a wafering blade with great difficulty.
Would anyone be familiar with this unit? Can we get blades to section samples upwards of approximately 1/2 inch in diameter?
Thanks for any help.
Alan Stone
==============================Original Headers============================== 7, 19 -- From as-at-astonmet.com Mon Aug 14 20:19:24 2006 7, 19 -- Received: from outbound1.mail.tds.net (outbound1.mail.tds.net [216.170.230.91]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7F1JO14016683 7, 19 -- for {microscopy-at-microscopy.com} ; Mon, 14 Aug 2006 20:19:24 -0500 7, 19 -- Received: from outaamta01.mail.tds.net (outaamta01.mail.tds.net [216.170.230.31]) 7, 19 -- by outbound1.mail.tds.net (8.13.6/8.13.4) with ESMTP id k7F1JNWU027950 7, 19 -- for {microscopy-at-microscopy.com} ; Mon, 14 Aug 2006 20:19:23 -0500 7, 19 -- Received: from D95S7761.astonmet.com ([216.170.179.105]) 7, 19 -- by outaamta01.mail.tds.net with ESMTP 7, 19 -- id {20060815011923.INNR5875.outaamta01.mail.tds.net-at-D95S7761.astonmet.com} 7, 19 -- for {microscopy-at-microscopy.com} ; Mon, 14 Aug 2006 20:19:23 -0500 7, 19 -- Message-Id: {6.2.0.14.2.20060814195433.020a5fb8-at-pop.tds.net} 7, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 7, 19 -- Date: Mon, 14 Aug 2006 20:19:23 -0500 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- From: Alan Stone {as-at-astonmet.com} 7, 19 -- Subject: lkb 2188 microtome information 7, 19 -- Mime-Version: 1.0 7, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both alerch-at-mcw.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: alerch-at-mcw.edu Name: Alexandra Lerch-Gaggl
Question: We have a Leica TCS SP2 with a 568nm laser in our facility. We had the tube remanufactured recently and I find now that over the time of an experiment the laser output decreases significantly, so that the intense signal i had at the beginning of the session is so deminished that I completely loose the image. When I open the box, which houses the laser, the power supply and the ventilator (remark on the side: I am allowed to do that, since I direct the facility and take care of our equipment), I see the signal return to its original intensity in a matter of a minute. My question is now: Is this related to overheating due to unsufficient ventilation or ... what else? I would appreciate if you could share your ideas/solutions with me. Thank you.
Barbara L. Plowman wrote: ===================================================== We have a very small electron microscopy laboratory with 1970;s state of the art equipment (Philips 201 and ETEC Autoscan). I am concerned about the dwindling shortage and increased costs of film, chemistry and paper. I would like to know if either or both of these instruments could be easily converted to digial from film recording. I have not kept up with the ccd camera prices and someone told me they wondered if a good digital camera with an adapter would be available. What kind of reasonable options are there for the old but very functional TEM and SEM? Thanks for your advice. =====================================================
These are really two different issues. For the Philips 201 TEM, there are cameras available from several different manufacturers. For example, see URL http://www.sia-cam.com/ There is also the possibility of digial imaging plates. http://www.ditabis.de/
For the ETEC SEM, you can convert from analog to digital with a number of different software/hardware products, one being the Orion Frame Grabber System for SEMs. See URL http://www.2spi.com/catalog/instruments/ORION_Digital_Image_Acquisition_System.html
As is the case with most products in EM, there is a wide range of product pricing, depending on budget and application.
It is off the topic but these two instruments are excellent for teaching purposes. When a student learns how to master the operation of either, they really do know how a TEM or SEM really "works". There is no software to do the thinking for you........
Chuck
Disclaimer: SPI Supplies offers the Orion Frame Grabber System for SEMs via the website indicated above.
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
==============================Original Headers============================== 12, 26 -- From cgarber-at-2spi.com Mon Aug 14 21:33:50 2006 12, 26 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7F2XnDL005870 12, 26 -- for {microscopy-at-msa.microscopy.com} ; Mon, 14 Aug 2006 21:33:49 -0500 12, 26 -- Received: from yourb27fb1c401 (c-71-230-36-118.hsd1.pa.comcast.net [71.230.36.118]) 12, 26 -- (authenticated bits=0) 12, 26 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id k7F2XfxV000987 12, 26 -- for {microscopy-at-msa.microscopy.com} ; Mon, 14 Aug 2006 22:33:49 -0400 12, 26 -- X-IDV-FirstRcvd: c-71-230-36-118.hsd1.pa.comcast.net [71.230.36.118] 12, 26 -- X-IDV-HELO: yourb27fb1c401 12, 26 -- X-IDV-Authenticated-User: cgarber 12, 26 -- Message-ID: {026e01c6c013$3af93500$6401a8c0-at-yourb27fb1c401} 12, 26 -- From: "Garber, Charles A" {cgarber-at-2spi.com} 12, 26 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 12, 26 -- Subject: converting to digital 12, 26 -- Date: Mon, 14 Aug 2006 22:33:44 -0400 12, 26 -- MIME-Version: 1.0 12, 26 -- Content-Type: text/plain; 12, 26 -- format=flowed; 12, 26 -- charset="iso-8859-1"; 12, 26 -- reply-type=original 12, 26 -- Content-Transfer-Encoding: 7bit 12, 26 -- X-Priority: 3 12, 26 -- X-MSMail-Priority: Normal 12, 26 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2869 12, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 ==============================End of - Headers==============================
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Question: I am using Aurion protein-A conjugated to 10 nm gold beads to detect viral antigens in an infected cell line - ultrathin sectioned and immnunostained. I am seeing a capping at the poles of cell sections and am wondering if this can be due to the beads and/or to the presence of serum blocking antibodies. The EMS Immnunogold Product Newsletter talks of Ultra-small reagent use for "No capping or patching, each probe unit consists of a single ligand". By the way, I am not seeing capping with IgM conjugated to 10nm beads and am unsure at this time about IgG conjugated beads.
On Aug 14, 2006, at 4:30 AM, nizets2-at-yahoo.com wrote:
} 1) Can I use e diffraction to distinguish both types } of particles? (to verify the purity of the powder) } 2) What kind of information about the cristal } structure can e diffraction give me in this case? } 3) Can I detect a change -and which change- to the } cristal structure using this technique if the mineral } is heat and treated with strong acids? (which actually } modifies the structure) } Dear Stephane, Just an addition to Wharton's excellent response. If your specimen consists of crystals too small to isolate one or two, you will likely have a ring pattern--either a solid ring if the selected area contains very many crystals or a series of spots arranged on a ring if there are fewer crystals. Measuring the diameter of the ring and comparing it to that for a known substance will give you spacings, which you can fit to those in model structures, and changes in the relative intensities of the rings can give structural information also (This could be due to slight atomic displacements that do not affect the unit cell, but change the relationships of the scattering from the atoms in the crystal.) One of the recent publications from our lab (Wright, E. R., Iancu, C. V., Tivol, W. F., and Jensen, G. J., Observations on the Behavior of Vitreous Ice at ~82 and ~12 K. Journal of Structural Biology, (2006) in press.) used ED to determine the dose at which low density amorphous ice underwent a transition to high density amorphous ice, so you could expect to see similar changes. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Tue Aug 15 15:55:22 2006 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7FKtMhB012610 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 15 Aug 2006 15:55:22 -0500 4, 22 -- Received: from water-dog.caltech.edu (water-dog [192.168.1.26]) 4, 22 -- by earth-ox-postvirus (Postfix) with ESMTP id 177C3109AFE 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 15 Aug 2006 13:55:22 -0700 (PDT) 4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 4, 22 -- by earth-ox.its.caltech.edu (Postfix) with ESMTP id 4261A109B31 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 15 Aug 2006 13:55:16 -0700 (PDT) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v624) 4, 22 -- In-Reply-To: {200608141130.k7EBUZCV010760-at-ns.microscopy.com} 4, 22 -- References: {200608141130.k7EBUZCV010760-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {5f33891af715f7b0adde84f0aae90373-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] need infos about e diffraction in TEM 4, 22 -- Date: Tue, 15 Aug 2006 13:57:39 -0700 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.624) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.3.3 ==============================End of - Headers==============================
I have been imaging negatively stained virions and isolated viral proteins and was wondering if anyone has ever applied blind deconvolution to images of negatively stained samples? Are there any references or caveats? Thanks!
George
George P. Leser, PhD Dept. Biochemistry, Molecular Biology, and Cell Biology Northwestern University Hogan 2-100 2153 North Campus Drive Evanston, IL 60208
g-leser-at-northwestern.edu
==============================Original Headers============================== 8, 22 -- From g-leser-at-northwestern.edu Tue Aug 15 16:25:56 2006 8, 22 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7FLPuQS023253 8, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Aug 2006 16:25:56 -0500 8, 22 -- Received: from hal90003ecc03cf (dhcp-129-105-38-214.bmbcb.northwestern.edu [129.105.38.214]) 8, 22 -- by merle.it.northwestern.edu (Postfix) with SMTP id 447142F0B0 8, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Aug 2006 16:25:56 -0500 (CDT) 8, 22 -- Message-ID: {003001c6c0b1$694c72c0$d6266981-at-hal90003ecc03cf} 8, 22 -- From: "George P. Leser" {g-leser-at-northwestern.edu} 8, 22 -- To: "1A-Microscopy Listserv" {Microscopy-at-microscopy.com} 8, 22 -- Subject: deconvolution of negatively stained EM images 8, 22 -- Date: Tue, 15 Aug 2006 16:26:02 -0500 8, 22 -- MIME-Version: 1.0 8, 22 -- Content-Type: text/plain; 8, 22 -- format=flowed; 8, 22 -- charset="iso-8859-1"; 8, 22 -- reply-type=original 8, 22 -- Content-Transfer-Encoding: 7bit 8, 22 -- X-Priority: 3 8, 22 -- X-MSMail-Priority: Normal 8, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2869 8, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2869 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jchampagne-at-caseforensicscorp.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, August 15, 2006 at 14:06:51 ---------------------------------------------------------------------------
Email: jchampagne-at-caseforensicscorp.com Name: Joseph Champagne
Organization: CASE Forensics - Used Microscopes
Education: 9-12th Grade High School
Location: Leavenworth, WA
Question: I would like some advise and/or recommendations on purchasing a good used microscope. What manufacturer(s), magnification power, light capability, where to buy, etc? Mono vs. Stereo?
I want to use it with my high school age children to look at plant and animal cells, and disected samples.
In theory, any conjugate which has more than one binding site, could induce capping or patching. This is true for all the conventional immuno-reagents, i.e. the big particles that likely have more than one specific binding molecule adsorbed or bound to the particle's surface. Ultra small particle based reagents, as quoted from the EMS Newsletter, are of a different built: they are proteins bound with one (or more) ultra small particles as opposed to a 10nm particle which is coated with more than one protein molecule. If you used serum in your blocking/incubation solutions the protein A gold reagent may have reacted with available immunoglobulins, which in the end may be causing the phenomenon as well.
Capping and patching may also be induced by the primary antibody, especially when more than 1 epitope is involved.
With more info I may be able to give you a better idea. Please contact me off-list.
Jan Leunissen
Aurion - President Electron Microscopy Research Advisor Costerweg 5 Dept Anatomy and Structural Biology 6702 A Wageningen Otago School of Medicine The Netherlands Dunedin, New Zealand phone 31-317-497676 phone 64-3-4795465 fax 31-317-415955 fax 64-3-4797254 http://www.aurion.nl http://anatomy.otago.ac.nz ------------------------------------------------------------------------ --------
==============================Original Headers============================== 13, 20 -- From leunissen-at-aurion.nl Tue Aug 15 20:44:47 2006 13, 20 -- Received: from fep04.xtra.co.nz (fep04.xtra.co.nz [210.54.141.242]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7G1ikZi015313 13, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Aug 2006 20:44:46 -0500 13, 20 -- Received: from [192.168.1.50] (really [222.152.164.98]) by fep04.xtra.co.nz 13, 20 -- with ESMTP 13, 20 -- id {20060816014436.XEKQ19789.fep04.xtra.co.nz-at-[192.168.1.50]} ; 13, 20 -- Wed, 16 Aug 2006 13:44:36 +1200 13, 20 -- In-Reply-To: {200608160321.AA262209640-at-aurion.nl} 13, 20 -- References: {200608160321.AA262209640-at-aurion.nl} 13, 20 -- Mime-Version: 1.0 (Apple Message framework v752.2) 13, 20 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 13, 20 -- Message-Id: {262522CB-EC3C-4D2B-98E4-C6866BB65965-at-aurion.nl} 13, 20 -- Cc: Microscopy-at-microscopy.com 13, 20 -- Content-Transfer-Encoding: 7bit 13, 20 -- From: Jan Leunissen {leunissen-at-aurion.nl} 13, 20 -- Subject: Re: [Microscopy] viaWWW: Capping or Patching 13, 20 -- Date: Wed, 16 Aug 2006 13:44:35 +1200 13, 20 -- To: kmoulton-at-usm.maine.edu 13, 20 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
Hi I need help in processing lymph nodes for TEM. I need a specific protocol for Lymph Nodes. The protocol that I'm using hasn't worked for this tissue. This protocol works with other tissues such as skin and heart 1- chemical fixation (glutaraldehyde + formaldehyde + tannic acid in cacodylate buffer for one hour) 2- secondary fixation with osmium 3- uranyl acetate overnight 4- dehydrations with increasing concentrations of ethanol (20 minutes each step) 5- resin infiltration with increasing concentration of resin (1 hour each step) 6- overnight incubation with 100% resin 7- next day 1 hour incubation with 100% resin 8- polymerization of the blocks containing the samples for 2 days at 60 degrees celsius.
Thanks!
Jaime
Jaime Llodra Graduate Student Sackler Institute NYU School of Medicine NY, NY 10016
==============================Original Headers============================== 4, 23 -- From jal490-at-nyu.edu Tue Aug 15 20:51:02 2006 4, 23 -- Received: from mx4.nyu.edu (MX4.NYU.EDU [128.122.108.244]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7G1p15k025545 4, 23 -- for {Microscopy-at-Microscopy.com} ; Tue, 15 Aug 2006 20:51:02 -0500 4, 23 -- Received: from mail.nyu.edu (H3.HOME.NYU.EDU [128.122.108.43]) 4, 23 -- by mx4.nyu.edu (8.13.6/8.13.6) with ESMTP id k7G1p1a6028380 4, 23 -- for {Microscopy-at-Microscopy.com} ; Tue, 15 Aug 2006 21:51:01 -0400 (EDT) 4, 23 -- Received: from [216.165.126.103] by mail.alt.home.nyu.edu (mshttpd); Tue, 4, 23 -- 15 Aug 2006 21:51:01 -0400 4, 23 -- From: Jaime A Llodra {jal490-at-nyu.edu} 4, 23 -- To: Microscopy-at-Microscopy.com 4, 23 -- Message-ID: {d55d8f9f32fb.44e241c5-at-mail.nyu.edu} 4, 23 -- Date: Tue, 15 Aug 2006 21:51:01 -0400 4, 23 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-4.03 (built Sep 22 4, 23 -- 2005) 4, 23 -- MIME-Version: 1.0 4, 23 -- Content-Language: en 4, 23 -- Subject: TEM - lymph node processing 4, 23 -- X-Accept-Language: en 4, 23 -- Priority: normal 4, 23 -- Content-Type: text/plain; charset=us-ascii 4, 23 -- Content-Disposition: inline 4, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi, I would appreciate the advice on the thioflavin-S staining. I have two precious sections which were previously studied for presence of inorganic deposits. The 40 micron thick sections from 4% PFA fixed brain tissue were mounted on slide, air-dried, and evaluated by x-ray spectroscopy. Now I would like to demonstrate amyloid but do not want to take any chances with the very important sample. I am looking for the best protocol to use on the sections ?abused? by air-drying. Thank you for your consideration, Albina
-- MIKHAYLOVA,ALBINA, PhD Post Doctoral Research Associate Materials Science and Engineering University of Florida
==============================Original Headers============================== 4, 21 -- From amich-at-ufl.edu Tue Aug 15 21:45:27 2006 4, 21 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7G2jQJ7003922 4, 21 -- for {Microscopy-at-Microscopy.com} ; Tue, 15 Aug 2006 21:45:27 -0500 4, 21 -- Received: from osgjas01.cns.ufl.edu (osgjas01.cns.ufl.edu [128.227.74.131]) 4, 21 -- by smtp.ufl.edu (8.13.7/8.13.7/2.5.9) with ESMTP id k7G2jMKZ1974384 4, 21 -- for {Microscopy-at-Microscopy.com} ; Tue, 15 Aug 2006 22:45:22 -0400 4, 21 -- Message-ID: {1121615292.49521155696322076.JavaMail.osg-at-osgjas01.cns.ufl.edu} 4, 21 -- Date: Tue, 15 Aug 2006 22:45:22 -0400 (EDT) 4, 21 -- From: "MIKHAYLOVA,ALBINA" {amich-at-ufl.edu} 4, 21 -- To: Microscopy-at-Microscopy.com 4, 21 -- Subject: thioflavin-S 4, 21 -- MIME-Version: 1.0 4, 21 -- Content-Type: text/plain; format=flowed; charset=us-ascii 4, 21 -- Content-Transfer-Encoding: 7bit 4, 21 -- X-Mailer: GatorMail WebMail (http://GatorMail.sf.net/) 4, 21 -- X-Originating-IP: 68.220.153.163 [68.220.153.163] 4, 21 -- X-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 4, 21 -- X-UFL-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 4, 21 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 4, 21 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
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I'll be happy to provide specific advice, but I need to know more about your needs & experience. I'm reluctant to recommend a used scope unless you have enough experience to recognize problems, and to fix them. Does "plant and animal cells" mean purchased, prepared slides? And what do you mean by "dissected samples"? What is your budget?
Although the microscope-buying advice on the MICRO website (URL below) is intended for a younger age group, you may find it a useful place to begin. There are several books in the MICRO bibliography that can help; again, I need to know more to make suggestions.
Caroline
-- Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 5, 19 -- From schooley-at-mcn.org Tue Aug 15 23:50:47 2006 5, 19 -- Received: from dns4.mcn.org (dns4.mcn.org [216.150.240.31]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7G4olg8016555 5, 19 -- for {microscopy-at-microscopy.com} ; Tue, 15 Aug 2006 23:50:47 -0500 5, 19 -- Received: from [66.52.139.254] (helo=[10.0.1.2]) 5, 19 -- by dns4.mcn.org with esmtpa (Exim 4.60) 5, 19 -- (envelope-from {schooley-at-mcn.org} ) 5, 19 -- id J42QSJ-000HO6-4P; Tue, 15 Aug 2006 21:50:44 -0700 5, 19 -- Mime-Version: 1.0 5, 19 -- Message-Id: {a06200700c1084e7f6931-at-[10.0.1.2]} 5, 19 -- In-Reply-To: {200608160122.k7G1MtaK013462-at-ns.microscopy.com} 5, 19 -- References: {200608160122.k7G1MtaK013462-at-ns.microscopy.com} 5, 19 -- Date: Tue, 15 Aug 2006 21:44:17 -0700 5, 19 -- To: jchampagne-at-caseforensicscorp.com 5, 19 -- From: Caroline Schooley {schooley-at-mcn.org} 5, 19 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: Used Microscopes 5, 19 -- with my high school 5, 19 -- Cc: microscopy-at-microscopy.com 5, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
At 20:54 15/08/2006 -0500, you wrote: hi Jaime, If you were more specific it would be easier to help.
In what way has your current protocol not worked, e.g. did the blocks not section well? Is the ultrastructural preservation poor? Was there a lot of deposit on the sections?
I presume you are using epoxy resin processing, in which case I suggest the following:
Make sure you are working with tissue of the correct dimensions, particularly the thickness which should not exceed 1mm. Next I would leave out the tanic acid and formaldehyde and fix in fresh ultrastructural grade gluteraldehyde (2 - 2.5% in 0.1 M phosphtae or cac buffer) overnight at 4'C. Buffer rinse and secondary fix in 1% OsO4. Cut way down on the U/A time to about 30mins in 1% aqueous. Your dehydrations times are OK if you are using thin slices of node but make sure the final dehydrant is dry by keeping your ethanol or acetone over a molecular seive. If you are using ethanol you must follow this with a link reagent such as proylene oxide and use this to mix with the resin for your infiltrations. If using acetone you dont need a link reagent as you can dilute your resin infiltration solns with acetone. If you are having sectioning problems, a bit of heat (but don't exceed 30 mins at 60'C) plus vacuum helps with the infiltration.
Hope this helps, but get back to me with specifics if you need any more guidance.
Alastair
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Alastair McKinnon Histology & EM Facility Manager University of Aberdeen, Institute of Medical Science Foresterhill, Aberdeen, AB25 2ZD tel: +44(0) 1224 552923 (office); +44(0) 1224 555911 (lab) fax: +44(0) 1224 559533; email: a.d.mckinnon-at-abdn.ac.uk www.abdn.ac.uk/ims/h-em
==============================Original Headers============================== 12, 18 -- From a.d.mckinnon-at-abdn.ac.uk Wed Aug 16 03:01:35 2006 12, 18 -- Received: from mailhub1.abdn.ac.uk (mailhub1.abdn.ac.uk [139.133.7.28]) 12, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7G81Z9k029940 12, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 16 Aug 2006 03:01:35 -0500 12, 18 -- Received: from med-0069.ims.abdn.ac.uk ([139.133.159.90] helo=med-0069.abdn.ac.uk) 12, 18 -- by mailhub1.abdn.ac.uk with esmtp (Exim 4.52) 12, 18 -- id 1GDGLG-0000fE-EN; Wed, 16 Aug 2006 09:01:34 +0100 12, 18 -- Message-Id: {5.2.1.1.0.20060816084017.011188a8-at-mailms.abdn.ac.uk} 12, 18 -- X-Sender: pat081-at-mailms.abdn.ac.uk 12, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 12, 18 -- Date: Wed, 16 Aug 2006 09:01:35 +0100 12, 18 -- To: jal490-at-nyu.edu 12, 18 -- From: Alastair McKinnon {a.d.mckinnon-at-abdn.ac.uk} 12, 18 -- Subject: Re: [Microscopy] TEM - lymph node processing 12, 18 -- Cc: Microscopy-at-Microscopy.Com 12, 18 -- In-Reply-To: {200608160154.k7G1sWKD002024-at-ns.microscopy.com} 12, 18 -- Mime-Version: 1.0 12, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Thanks for the tip, but this book is not available at Amazon. I did a search on the www but I cannot find a distributor (it is probably too old).
I already spent one day on the link given by Sousan (very nice).
Would there be another good reference book (more recent perhaps) which does the same job as Hirsch's?
Regards,
Stephane
--- Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} wrote:
} Stephane } } Purchase a copy of Hirsch's book. } Go to Amazon.com. } Search for "transmission electron hirsch". } } regards, } } Jim } } } From mail-at-ns.microscopy.com Mon Aug 14 07:30:49 } 2006 } } Date: Mon, 14 Aug 2006 06:33:44 -0500 } } To: jquinn-at-www.matscieng.sunysb.edu } } From: nizets2-at-yahoo.com } } Reply-to: nizets2-at-yahoo.com } } X-Resent-From: "Microscopy Listserver" } {microscopy-at-microscopy.com} } } Subject: [Microscopy] need infos about e } diffraction in TEM } } Errors-To: } MicroscopyListSpamFilter-at-microscopy.com } } X-lewp: MicroscopyListSpam NAGS } } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Dear listers, } } } } Although I understand the principle behind } electron } } diffraction in TEM I have no idea about the kind } of } } informations this technique gives and how to } interpret } } the diffraction pattern. } } If you could give me a www address which explains } that } } I would be much grateful. } } } } Now a very practical problem could be perhaps } used as } } an example: we have a mixture of aluminosilicate } } mineral (I have cut 70 nm sections) with approx. } 90% } } of mordenite and 10% quarz. } } 1) Can I use e diffraction to distinguish both } types } } of particles? (to verify the purity of the } powder) } } 2) What kind of information about the cristal } } structure can e diffraction give me in this case? } } 3) Can I detect a change -and which change- to } the } } cristal structure using this technique if the } mineral } } is heat and treated with strong acids? (which } actually } } modifies the structure) } } } } We have also a tilt stage for tomography. Can } this } } bring further informations? } } } } Regards, } } } } Stephane } } } } } } } __________________________________________________ } } Do You Yahoo!? } } Tired of spam? Yahoo! Mail has the best spam } protection around } } http://mail.yahoo.com } } } } ==============================Original } Headers============================== } } 8, 18 -- From nizets2-at-yahoo.com Mon Aug 14 } 06:30:17 2006 } } 8, 18 -- Received: from } web37406.mail.mud.yahoo.com } (web37406.mail.mud.yahoo.com [209.191.91.138]) } } 8, 18 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with SMTP id } k7EBUGao010591 } } 8, 18 -- for {microscopy-at-microscopy.com} ; Mon, } 14 Aug 2006 06:30:17 -0500 } } 8, 18 -- Received: (qmail 81606 invoked by uid } 60001); 14 Aug 2006 11:30:15 -0000 } } 8, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; } c=nofws; } } 8, 18 -- s=s1024; d=yahoo.com; } } 8, 18 -- } h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; } } 8, 18 -- } b=rlmt7902ZNRzFTTFfQF7ruMzaWPunDe6Tu9YxDJ2pD9W3I+BsUwz4JFylLSb1NbV5Y1qFqa1jK6YE5BUUAa9EVhASSCrla6W/fKuGhTPt6o7J7paQwTF6sqlqLCbo9YrFe2LwGUS/eGKK8kO3I8ZVczxOMyA1VvnaLJ+c9MHHSw= } ; } } 8, 18 -- Message-ID: } {20060814113015.81604.qmail-at-web37406.mail.mud.yahoo.com} } } 8, 18 -- Received: from [80.122.101.102] by } web37406.mail.mud.yahoo.com via HTTP; Mon, 14 Aug } 2006 04:30:15 PDT } } 8, 18 -- Date: Mon, 14 Aug 2006 04:30:15 -0700 } (PDT) } } 8, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} } } 8, 18 -- Subject: need infos about e diffraction } in TEM } } 8, 18 -- To: microscopy-at-microscopy.com } } 8, 18 -- MIME-Version: 1.0 } } 8, 18 -- Content-Type: text/plain; } charset=iso-8859-1 } } 8, 18 -- Content-Transfer-Encoding: 8bit } } ==============================End of - } Headers============================== } } }
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==============================Original Headers============================== 9, 20 -- From nizets2-at-yahoo.com Thu Aug 17 01:48:40 2006 9, 20 -- Received: from web37408.mail.mud.yahoo.com (web37408.mail.mud.yahoo.com [209.191.91.140]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k7H6mdnF020219 9, 20 -- for {microscopy-at-microscopy.com} ; Thu, 17 Aug 2006 01:48:39 -0500 9, 20 -- Received: (qmail 65219 invoked by uid 60001); 17 Aug 2006 06:48:39 -0000 9, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 20 -- s=s1024; d=yahoo.com; 9, 20 -- h=Message-ID:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 9, 20 -- b=46+tg4vtVqYk4X30p1FZiDj9JUtYOUZ70i9iC+IOR1ImeYHrIORqoXUOjWeXobDcVAU02OuMvDMZ4v7ppX2u88xgiQDYbIyQvQgXztDbAN583tchZWrl6Hg9mt/w7+WBRWOLe0BnTVxaZ/lKCbshR91+u4UvaKgZf7+dhg1OGIU= ; 9, 20 -- Message-ID: {20060817064839.65217.qmail-at-web37408.mail.mud.yahoo.com} 9, 20 -- Received: from [80.122.101.102] by web37408.mail.mud.yahoo.com via HTTP; Wed, 16 Aug 2006 23:48:39 PDT 9, 20 -- Date: Wed, 16 Aug 2006 23:48:39 -0700 (PDT) 9, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 9, 20 -- Subject: Re: [Microscopy] need infos about e diffraction in TEM 9, 20 -- To: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 9, 20 -- Cc: microscopy-at-microscopy.com 9, 20 -- In-Reply-To: {200608141344.k7EDiSc09038-at-www.matscieng.sunysb.edu} 9, 20 -- MIME-Version: 1.0 9, 20 -- Content-Type: text/plain; charset=iso-8859-1 9, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
We get asked this fairly regularly by schools and parents (so we have stock replies). Often professional microscopists aren't the best source of information for cheap microscope's as all our optical systems are always over £10,000, and most lab mainstays like our three confocal microscopes come in at nearer £200,000 each (and users still complain about image quality). I assume you are asking about home use with your own kids.
Cheap microscopes under £100 are always a disappointment and toy (often called student) microscopes can be very poor. You can easily buy second-hand via ebay, but again there are risks that the optics will be damaged or simply very dirty and difficult to clean and you may make an expensive mistake. Look for old branded 'laboratory' microscopes e.g. Bausch & Lomb, Prior, Leitz, Reichert, Baker, but probably not Tasco toys. Famous existing brands like Zeiss and Olympus attract a high premium. The sellers are often not microscopists though, and many are sold as collector's items and not for 'scientific' use. Also try any local microscope enthusiast clubs - they aren't as common as the many excellent astronomy [telescope] clubs but they are about and have knowledgeable enthusiasts with an eye for low cost quality systems.
There are suppliers geared up to providing cheaper microscopes for schools, so you can ask around at school's science departments, but expect to pay nearer £500 each for a quality setup (although with those like bottom end Meade [www.meade.com] at around £100 you can see something at low mag (~20x objective i.e. around 180x mag) with a quality stained section.
For pond life etc. a stereoscopic 'dissecting' microscope (40x to 120x mag) is ideal, and of course you can get a really long way with a good magnifying glass (not the really small hi-mag cheap lens ones, try before you buy) - I have a few excellent ones at home for £1 and a good low mag Osram one that includes an illuminating halogen bulb at £8. In general I would say a good stereo dissecting microscope is a good choice for kids as it's great for viewing living things and enlarges what you can see already - look for 40x rather than 4x though.
Generally prepared slides can rapidly get very boring for under 14s, but living or unusual things (even hamster fur) always attract an audience. Also try your flatbed film scanner (not LiDe and from around £60 upwards) that will be good for looking at soft static things: leaves, fruit, nuts, household objects (scan at max resolution and try reflected and 'film' mode). In the UK there are sites like http://www.wedgwood-group.com/microscopes_digital.htm that cater for schools and colleges, providing standard compound and stereo microscopes as well as cheap PC video based microscope solutions where the whole class can look at a computer screen with some pushing and shoving. Best to try them on 'approval' as many cheap microscopes can be disappointing if you expect too much.
Excellent pre-prepared stained slides of plant stems and leaves or bits of rats, insects etc.. can be bought via ebay, but they tend to be expensive and are easily broken by any age-group. Mounted slides keep well though, so 'vintage' ones even from 50 years ago can still look OK - most schools will have some specimen slides knocking about.
At home and our Primary school (under 11) we use the Digital Blue QX-5 (£70) - it's fun but pretty useless for serious microscope work as it's so low res (but at 640 x 480 better than the old Intel QX-3 it replaced). It puts the image on a PC screen. I have one at home for my kids (boy 10 and girl 12) but it only gets occasional use now the novelty has worn off. See http://micro.magnet.fsu.edu/optics/intelplay/index.html (not updated for the QX-5 but all applies - the site even discusses ways of contrast enhancement etc..). Once on the PC the 640x480 images can be manipulated and pasted etc, and the QX-5 does time-lapse for things like crystal growth. living plants growing and small animals. The similar but far better built Olympus MIC-D was great but being over £500 it was just too expensive for most schools and is now discontinued - there are other similar budget systems about though.
The macro on a good digital camera (like the image stabilised 5MP Canon S2IS going cheap at £200 over here - http://www.dpreview.com/reviews/canons2is) is also worth a try, particularly with a small tripod and halogen bendy desk lamp if very close-up, but I'm not sure I'd like a class with 20 boys near my Olympus E500 digital SLR system though. You can get quite reasonable pictures by resting a small compact digital camera lens against the eyepiece of a microscope. Plus you can the camera for normal photography when you bored with microscopy.
By the way, if you get a microscope, do try growing crystals on a slide, a few drops of a saturated solution of salt (NaCl) or copper sulphate will grow superb crystals on the surface of a slide when viewed under a microscope (but it takes a few hours for the crystals to form and they often look best before the liquids all gone). Just make sure they don't drive the objective tips into the solution. It's not biology but its fun.
Regards
Keith
Try looking in Amazon.com for decent microscope books with lots of pictures (and they have a good customer review system). Plus try web searches for general sites like these (and for more specific topics) :
http://www.101science.com/Microscope.htm http://micro.magnet.fsu.edu/ http://www.microscopy-uk.org.uk/index.html http://www.btinternet.com/~stephen.durr/ http://www.mccroneatlas.com http://www.diatoms.co.uk [for fun images made of diatoms]
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
-----Original Message----- X-from: schooley-at-mcn.org [mailto:schooley-at-mcn.org] Sent: 16 August 2006 05:55 To: keith.morris-at-ucl.ac.uk
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I'll be happy to provide specific advice, but I need to know more about your needs & experience. I'm reluctant to recommend a used scope unless you have enough experience to recognize problems, and to fix them. Does "plant and animal cells" mean purchased, prepared slides? And what do you mean by "dissected samples"? What is your budget?
Although the microscope-buying advice on the MICRO website (URL below) is intended for a younger age group, you may find it a useful place to begin. There are several books in the MICRO bibliography that can help; again, I need to know more to make suggestions.
Caroline
-- Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 5, 19 -- From schooley-at-mcn.org Tue Aug 15 23:50:47 2006 5, 19 -- Received: from dns4.mcn.org (dns4.mcn.org [216.150.240.31]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7G4olg8016555 5, 19 -- for {microscopy-at-microscopy.com} ; Tue, 15 Aug 2006 23:50:47 -0500 5, 19 -- Received: from [66.52.139.254] (helo=[10.0.1.2]) 5, 19 -- by dns4.mcn.org with esmtpa (Exim 4.60) 5, 19 -- (envelope-from {schooley-at-mcn.org} ) 5, 19 -- id J42QSJ-000HO6-4P; Tue, 15 Aug 2006 21:50:44 -0700 5, 19 -- Mime-Version: 1.0 5, 19 -- Message-Id: {a06200700c1084e7f6931-at-[10.0.1.2]} 5, 19 -- In-Reply-To: {200608160122.k7G1MtaK013462-at-ns.microscopy.com} 5, 19 -- References: {200608160122.k7G1MtaK013462-at-ns.microscopy.com} 5, 19 -- Date: Tue, 15 Aug 2006 21:44:17 -0700 5, 19 -- To: jchampagne-at-caseforensicscorp.com 5, 19 -- From: Caroline Schooley {schooley-at-mcn.org} 5, 19 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: Used Microscopes 5, 19 -- with my high school 5, 19 -- Cc: microscopy-at-microscopy.com 5, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 33, 27 -- From keith.morris-at-ucl.ac.uk Thu Aug 17 04:23:33 2006 33, 27 -- Received: from vscani-a.ucl.ac.uk (vscani-a.ucl.ac.uk [144.82.108.29]) 33, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7H9NWRx000615 33, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Aug 2006 04:23:32 -0500 33, 27 -- Received: from cell23.ioo.ucl.ac.uk ([144.82.134.123] helo=keithhigrade) 33, 27 -- by vscani-a.ucl.ac.uk with esmtp (Exim 4.60) 33, 27 -- (envelope-from {keith.morris-at-ucl.ac.uk} ) 33, 27 -- id 1GDe64-0001dL-MG; Thu, 17 Aug 2006 10:23:28 +0100 33, 27 -- From: "Keith J Morris" {keith.morris-at-ucl.ac.uk} 33, 27 -- To: {jchampagne-at-caseforensicscorp.com} 33, 27 -- Cc: {Microscopy-at-microscopy.com} 33, 27 -- Subject: RE: [Microscopy] Re: [Filtered] AskAMicroscopist: Used Microscopes 33, 27 -- Date: Thu, 17 Aug 2006 10:23:27 +0100 33, 27 -- Message-ID: {001501c6c1de$cc1cfc70$7b865290-at-keithhigrade} 33, 27 -- MIME-Version: 1.0 33, 27 -- Content-Type: text/plain; 33, 27 -- charset="iso-8859-1" 33, 27 -- X-Mailer: Microsoft Office Outlook 11 33, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 33, 27 -- Thread-Index: AcbA8CYRO86f57EFRpe8Ilig1fK6ogA6gu0g 33, 27 -- In-Reply-To: {200608160455.k7G4t0xa023549-at-ns.microscopy.com} 33, 27 -- X-UCL-MailScanner-Information: Please contact the UCL Helpdesk, helpdesk-at-ucl.ac.uk for more information 33, 27 -- X-UCL-MailScanner: Found to be clean 33, 27 -- X-UCL-MailScanner-From: keith.morris-at-ucl.ac.uk 33, 27 -- X-Spam-Status: No 33, 27 -- Content-Transfer-Encoding: 8bit 33, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k7H9NWRx000615 ==============================End of - Headers==============================
A cautionary note about buying lab equipment on e-bay,etc:
A few years ago, we had a rash of small and not-so-small instrumentation going missing (pipettors, and microscope components). An astute pot-doc who was playing "let's see if e-bay any any _____" came across listings of items for sale, all by one seller, that very closely matched the missing material. He reported it to the med school's security department who investigated. It turned out that another post-doc had been spiriting items out of his lab and the labs of his PI's collaborators and supplementing his income by selling them on e-bay. e-bay was EXTREMELY cooperative about helping with the investigation and, needless to say that fellow's career was brought to a crashing halt. e-bay has grown so large that although it makes efforts to control what's on its site, clearly, it cannot do so 100%. I don't know the final outcome with regard to the stolen materials.
Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175 http://www.cornellcelldevbiology.org http://www.cornellbiochem.org
==============================Original Headers============================== 3, 23 -- From lcgould-at-med.cornell.edu Thu Aug 17 08:15:40 2006 3, 23 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7HDFdPY030757 3, 23 -- for {microscopy-at-microscopy.com} ; Thu, 17 Aug 2006 08:15:40 -0500 3, 23 -- Received: from mpx2.med.cornell.edu (pc113142-10.med.cornell.edu [140.251.11.119]) 3, 23 -- by smtp-gw2.med.cornell.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id k7HDFZx9027395 3, 23 -- for {microscopy-at-microscopy.com} ; Thu, 17 Aug 2006 09:15:36 -0400 (EDT) 3, 23 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23]) 3, 23 -- by mpx2.med.cornell.edu 3, 23 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 3, 23 -- with ESMTPA id {0J45003A78TZX220-at-mpx2.med.cornell.edu} for 3, 23 -- microscopy-at-microscopy.com; Thu, 17 Aug 2006 09:15:35 -0400 (EDT) 3, 23 -- Date: Thu, 17 Aug 2006 09:15:40 -0400 3, 23 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 3, 23 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: Used Microscopes 3, 23 -- In-reply-to: {200608170924.k7H9OKKr001891-at-ns.microscopy.com} 3, 23 -- Sender: lcgould-at-med.cornell.edu 3, 23 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 23 -- Message-id: {p06230900c10a1a54da70-at-[140.251.48.23]} 3, 23 -- MIME-version: 1.0 3, 23 -- Content-type: text/plain; charset=us-ascii; format=flowed 3, 23 -- References: {200608170924.k7H9OKKr001891-at-ns.microscopy.com} 3, 23 -- X-PMX-Version: 5.2.0.264296, Antispam-Engine: 2.4.0.264935, Antispam-Data: 2006.8.17.55442 ==============================End of - Headers==============================
Has anyone used this particular microscope EM-109 (Zeiss) and have had any problems with it? I can't seem to get the filament to work, and the condenser moves when I go to spread the beam out. Anyone have any suggestions as to how to fix it?
Yarn Dept of Agriculture
==============================Original Headers============================== 3, 19 -- From addlvirology-at-mail.agri.state.oh.us Thu Aug 17 08:36:10 2006 3, 19 -- Received: from ODANTMAIL.agri.state.oh.us (mail.agri.state.oh.us [198.234.149.236]) 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k7HDa97f011571 3, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Aug 2006 08:36:09 -0500 3, 19 -- Received: from 198.234.153.194 by ODANTMAIL.agri.state.oh.us (InterScan E-Mail VirusWall NT); Thu, 17 Aug 2006 09:30:54 -0400 3, 19 -- Received: from EAG13221 [172.16.6.11] by mail.agri.state.oh.us with ESMTP 3, 19 -- (SMTPD-8.21) id A1460200; Thu, 17 Aug 2006 09:38:14 -0400 3, 19 -- From: "addlvirology" {addlvirology-at-mail.agri.state.oh.us} 3, 19 -- To: {Microscopy-at-microscopy.com} 3, 19 -- Subject: EM-109 3, 19 -- Date: Thu, 17 Aug 2006 09:35:59 -0400 3, 19 -- MIME-Version: 1.0 3, 19 -- Content-Type: text/plain; 3, 19 -- charset="us-ascii" 3, 19 -- Content-Transfer-Encoding: 7bit 3, 19 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 3, 19 -- Thread-Index: AcbCAhL/QlttoiuUQ2CApHB7tMXKHA== 3, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1165 3, 19 -- Message-Id: {200608170938156.SM02148-at-EAG13221} ==============================End of - Headers==============================
Regards purchase on eBay of 'hot' items, always ask the seller for serial number information. This can provide information about the age and legitimacy of the item. If the seller will not provide information, it's probably best to not purchase.
As a further cautionary note - you have no guarantee that the equipment you purchase will work properly. Make sure that the seller is reputable and that you can service or repair whatever you buy. We recently purchased a microcentrifuge using eBay to replace equipment which went to another lab - totally legitimately, the other person had paid for it and the group was growing. The microcentrifuge works well. As a result, I am now thinking about purchasing variable volume pipetters. Again, to replace equipment which was moved to the other lab. In the first case, I followed the caveats of getting a history before bidding, knowing what the item cost new, knowing what it may cost to repair or calibrate the equipment, and establishing a maximum amount I'm was willing to bid. I will do the same if we decide to actually purchase pipetters.
Finally, remember that in many ways eBay is a game where people may get caught up in the thrill of the chase, and most of that chase occurs in the last 10 minutes. Stick to that maximum you set or you may get caught up in the game and pay too much.
Paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax: 204-789-3926
==============================Original Headers============================== 10, 20 -- From paul_hazelton-at-umanitoba.ca Thu Aug 17 09:46:21 2006 10, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7HEkJ9Q025211 10, 20 -- for {microscopy-at-microscopy.com} ; Thu, 17 Aug 2006 09:46:20 -0500 10, 20 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 10, 20 -- (authenticated bits=0) 10, 20 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id k7HEkHiZ009533; 10, 20 -- Thu, 17 Aug 2006 09:46:18 -0500 (CDT) 10, 20 -- Message-ID: {44E48135.2030802-at-umanitoba.ca} 10, 20 -- Date: Thu, 17 Aug 2006 09:46:13 -0500 10, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 10, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 10, 20 -- X-Accept-Language: en-us, en 10, 20 -- MIME-Version: 1.0 10, 20 -- CC: Microscopy Listserver {microscopy-at-microscopy.com} 10, 20 -- Subject: Re: [Microscopy] Re: [Filtered] AskAMicroscopist: Used Microscopes 10, 20 -- References: {200608171318.k7HDI9pv002426-at-ns.microscopy.com} 10, 20 -- In-Reply-To: {200608171318.k7HDI9pv002426-at-ns.microscopy.com} 10, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Does anyone know if dispersants used in making cement (yup...new sample for us biologists!) can act as cryo-protectants when freezing the sample using HPS? The dispersants are proprietary comb-type polycarboxylates with straight backbones and parallel short chains extending outward.
Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 4, 21 -- From dsherman-at-purdue.edu Thu Aug 17 10:26:48 2006 4, 21 -- Received: from 1061exfe03.adpc.purdue.edu (1061exfe03.adpc.purdue.edu [128.210.63.225]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7HFQma5007928 4, 21 -- for {microscopy-at-microscopy.com} ; Thu, 17 Aug 2006 10:26:48 -0500 4, 21 -- Received: from exchange.purdue.edu ([172.21.6.24]) by 1061exfe03.adpc.purdue.edu with Microsoft SMTPSVC(6.0.3790.211); 4, 21 -- Thu, 17 Aug 2006 11:26:47 -0400 4, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 4, 21 -- Thu, 17 Aug 2006 15:26:47 +0000 4, 21 -- User-Agent: Microsoft-Entourage/11.2.5.060620 4, 21 -- Date: Thu, 17 Aug 2006 11:26:46 -0400 4, 21 -- Subject: Cryo-protectant for cement 4, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 4, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 4, 21 -- Message-ID: {C10A02F6.137B7%dsherman-at-purdue.edu} 4, 21 -- Thread-Topic: Cryo-protectant for cement 4, 21 -- Thread-Index: AcbCEYzay2zl1C4EEduGmQARJN08Mg== 4, 21 -- Mime-version: 1.0 4, 21 -- Content-type: text/plain; 4, 21 -- charset="US-ASCII" 4, 21 -- Content-transfer-encoding: 7bit 4, 21 -- X-OriginalArrivalTime: 17 Aug 2006 15:26:47.0993 (UTC) FILETIME=[8E0A9A90:01C6C211] ==============================End of - Headers==============================
The University of Oregon is hosting a workshop for Energy Dispersive Analysis (EDS) which will focus of quantitative phase mapping and low voltage (high resolution) analysis of materials on the scanning electron microscope and electron microprobe analyzer. Topics will include identification of minor and trace phases and contaminates, phase distribution and modal abundance and quantitation of small particles and other embedded phases. This workshop will be a hands on exploration of the state of the art on several instruments offering the latest instrumental breakthroughs for quantitative analysis and x-ray mapping capabilities with one of the world's leading experts: Dale Newbury from the National Institute of Standards and Technology.
1. Zeiss Ultra with Oxford Inca 2. FEI Quanta with Bruker XFlash 3. Cameca SX100 with Thermo System Six
Attendance is limited! Additional morning presentations are welcome- please contact John Donovan directly.
EDS Spectrum Imaging and Low Voltage (High Resolution) Analysis, University of Oregon, Eugene, OR
3 day workshop by Dale Newbury (National Institute of Standards & Technology), September, 12-14, 2006
Morning 1 talk: "Silicon Drift Detectors: Energy Dispersive X-ray Spectrometry and X-ray Spectrum Imaging at Output Count Rates Above 100 kHz, and What to Do with All This Data"
Afternoon Demos: * Spectral Imaging and Mapping with a conventional EDS (Oxford and Thermo) * Bruker Quad SDD and Thermo SDD doing high speed x-ray spectrum image mapping on an FEI Quanta SEM * LISPIX hands-on software lab, with pre-recorded x-ray spectrum image data sets.
Morning 2 talk: "Challenges to Successful EDS X-ray Microanalysis in the Low Beam Energy (E0 { 5 keV) Regime"
Afternoon demos: * Low Beam Energy SEM/EDS on selected specimens, e.g., SiC, BaTiO3, etc. * Demonstration of conventional WDS in the low beam energy regime with BaTiO3 scanning the TiL-O K and BaM spectral regions.
Morning 3 talk: "The Perils of Automatic EDS Analysis: Blunders in Automated Peak Identification of Major, Minor, and Trace Constituents and the Reality of Standardless Analysis"
Afternoon demo: * Testing Automatic Peak ID and Standardless Analysis with commercial software in the Laboratory * User suggestions for testing (bring your own samples)
Check this page for registration and further details: http://epmalab.uoregon.edu/eds_workshop.htm
Respond to John Donovan (donovan-at-uoregon.edu) to confirm your participation. Registration is required by September 1, 2006. More to come...
==============================Original Headers============================== 15, 21 -- From donovan-at-uoregon.edu Thu Aug 17 16:30:24 2006 15, 21 -- Received: from smtp.uoregon.edu (mserv3.uoregon.edu [128.223.142.101]) 15, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7HLUNwO026397 15, 21 -- for {Microscopy-at-Microscopy.Com} ; Thu, 17 Aug 2006 16:30:23 -0500 15, 21 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.92.75]) 15, 21 -- (authenticated bits=0) 15, 21 -- by smtp.uoregon.edu (8.13.7/8.13.7) with ESMTP id k7HLUNtO031060 15, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 15, 21 -- Thu, 17 Aug 2006 14:30:23 -0700 15, 21 -- Message-Id: {7.0.1.0.0.20060817142610.03841d88-at-uoregon.edu} 15, 21 -- Message-Id: {7.0.1.0.0.20060817142352.03841268-at-uoregon.edu} 15, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 15, 21 -- Date: Thu, 17 Aug 2006 14:30:16 -0700 15, 21 -- To: Microscopy-at-Microscopy.Com 15, 21 -- From: John Donovan {donovan-at-uoregon.edu} 15, 21 -- Subject: EDS/Spectrum Imaging/Low Voltage Analysis Workshop, Eugene, OR 15, 21 -- Cc: dale.newbury-at-nist.gov 15, 21 -- Mime-Version: 1.0 15, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 15, 21 -- X-Virus-Scanned: ClamAV 0.88.4/1677/Thu Aug 17 06:56:09 2006 on mserv3 15, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
We are looking for opportunities to learn the techniques of electron diffractometry (by TEM). We are working with a Tecnai G20 and the main purpose is the analysis of natural mineral samples. We will consider any proposition from workshop to on-site training. A very important limitation is geography: we have no desire to leave our good old Europa (we are based in Austria).
Stephane
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==============================Original Headers============================== 5, 18 -- From nizets2-at-yahoo.com Fri Aug 18 01:57:03 2006 5, 18 -- Received: from web37414.mail.mud.yahoo.com (web37414.mail.mud.yahoo.com [209.191.91.146]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k7I6v3oZ013219 5, 18 -- for {microscopy-at-microscopy.com} ; Fri, 18 Aug 2006 01:57:03 -0500 5, 18 -- Received: (qmail 98216 invoked by uid 60001); 18 Aug 2006 06:57:02 -0000 5, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 18 -- s=s1024; d=yahoo.com; 5, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 5, 18 -- b=iabXjwC63yxnnEBcw+wnNbXh+IQ6RnQrzLu5jCuhyfBKcC37mcdH9DhbO72LON/aB66wEAa8lG4nhjXsxw4ZnLG+a33tSFw2B+11LRWijaEOH/q7+Z72n1LPfThhlHaXUIbAUH/qpHLs9vLZ7fZWoUMOWrAASul0enA7ici0XhA= ; 5, 18 -- Message-ID: {20060818065702.98214.qmail-at-web37414.mail.mud.yahoo.com} 5, 18 -- Received: from [80.122.101.102] by web37414.mail.mud.yahoo.com via HTTP; Thu, 17 Aug 2006 23:57:02 PDT 5, 18 -- Date: Thu, 17 Aug 2006 23:57:02 -0700 (PDT) 5, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 18 -- Subject: workshop/courses about electron diffractometry 5, 18 -- To: microscopy-at-microscopy.com 5, 18 -- MIME-Version: 1.0 5, 18 -- Content-Type: text/plain; charset=iso-8859-1 5, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Not sure which Hirsch book jim had in mind but maybe one of :
TOPICS IN ELECTRON DIFFRACTION AND MICROSCOPY OF MATERIALS (1999) Electron Microscopy of Thin Crystals (1965)
(transmission isn't in the title..)
nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Thanks for the tip, but this book is not available at } Amazon. I did a search on the www but I cannot find a } distributor (it is probably too old). } } I already spent one day on the link given by Sousan } (very nice). } } Would there be another good reference book (more } recent perhaps) which does the same job as Hirsch's? } } Regards, } } Stephane } } --- Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} wrote: } } } Stephane } } } } Purchase a copy of Hirsch's book. } } Go to Amazon.com. } } Search for "transmission electron hirsch". } } } } regards, } } } } Jim } } } } } From mail-at-ns.microscopy.com Mon Aug 14 07:30:49 } } 2006 } } } Date: Mon, 14 Aug 2006 06:33:44 -0500 } } } To: jquinn-at-www.matscieng.sunysb.edu } } } From: nizets2-at-yahoo.com } } } Reply-to: nizets2-at-yahoo.com } } } X-Resent-From: "Microscopy Listserver" } } {microscopy-at-microscopy.com} } } } Subject: [Microscopy] need infos about e } } diffraction in TEM } } } Errors-To: } } MicroscopyListSpamFilter-at-microscopy.com } } } X-lewp: MicroscopyListSpam NAGS } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The } } Microscopy Society of America } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ---------------------------------------------------------------------------- } } } } } } Dear listers, } } } } } } Although I understand the principle behind } } electron } } } diffraction in TEM I have no idea about the kind } } of } } } informations this technique gives and how to } } interpret } } } the diffraction pattern. } } } If you could give me a www address which explains } } that } } } I would be much grateful. } } } } } } Now a very practical problem could be perhaps } } used as } } } an example: we have a mixture of aluminosilicate } } } mineral (I have cut 70 nm sections) with approx. } } 90% } } } of mordenite and 10% quarz. } } } 1) Can I use e diffraction to distinguish both } } types } } } of particles? (to verify the purity of the } } powder) } } } 2) What kind of information about the cristal } } } structure can e diffraction give me in this case? } } } 3) Can I detect a change -and which change- to } } the } } } cristal structure using this technique if the } } mineral } } } is heat and treated with strong acids? (which } } actually } } } modifies the structure) } } } } } } We have also a tilt stage for tomography. Can } } this } } } bring further informations? } } } } } } Regards, } } } } } } Stephane } } } } } } } } } } } __________________________________________________ } } } Do You Yahoo!? } } } Tired of spam? Yahoo! Mail has the best spam } } protection around } } } http://mail.yahoo.com } } } } } } ==============================Original } } Headers============================== } } } 8, 18 -- From nizets2-at-yahoo.com Mon Aug 14 } } 06:30:17 2006 } } } 8, 18 -- Received: from } } web37406.mail.mud.yahoo.com } } (web37406.mail.mud.yahoo.com [209.191.91.138]) } } } 8, 18 -- by ns.microscopy.com } } (8.12.11.20060308/8.12.8) with SMTP id } } k7EBUGao010591 } } } 8, 18 -- for {microscopy-at-microscopy.com} ; Mon, } } 14 Aug 2006 06:30:17 -0500 } } } 8, 18 -- Received: (qmail 81606 invoked by uid } } 60001); 14 Aug 2006 11:30:15 -0000 } } } 8, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; } } c=nofws; } } } 8, 18 -- s=s1024; d=yahoo.com; } } } 8, 18 -- } } } h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; } } } 8, 18 -- } } } b=rlmt7902ZNRzFTTFfQF7ruMzaWPunDe6Tu9YxDJ2pD9W3I+BsUwz4JFylLSb1NbV5Y1qFqa1jK6YE5BUUAa9EVhASSCrla6W/fKuGhTPt6o7J7paQwTF6sqlqLCbo9YrFe2LwGUS/eGKK8kO3I8ZVczxOMyA1VvnaLJ+c9MHHSw= } } ; } } } 8, 18 -- Message-ID: } } } {20060814113015.81604.qmail-at-web37406.mail.mud.yahoo.com} } } } 8, 18 -- Received: from [80.122.101.102] by } } web37406.mail.mud.yahoo.com via HTTP; Mon, 14 Aug } } 2006 04:30:15 PDT } } } 8, 18 -- Date: Mon, 14 Aug 2006 04:30:15 -0700 } } (PDT) } } } 8, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} } } } 8, 18 -- Subject: need infos about e diffraction } } in TEM } } } 8, 18 -- To: microscopy-at-microscopy.com } } } 8, 18 -- MIME-Version: 1.0 } } } 8, 18 -- Content-Type: text/plain; } } charset=iso-8859-1 } } } 8, 18 -- Content-Transfer-Encoding: 8bit } } } ==============================End of - } } Headers============================== } } } } } } } } __________________________________________________ } Do You Yahoo!? } Tired of spam? Yahoo! Mail has the best spam protection around } http://mail.yahoo.com } } ==============================Original Headers============================== } 9, 20 -- From nizets2-at-yahoo.com Thu Aug 17 01:48:40 2006 } 9, 20 -- Received: from web37408.mail.mud.yahoo.com (web37408.mail.mud.yahoo.com [209.191.91.140]) } 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k7H6mdnF020219 } 9, 20 -- for {microscopy-at-microscopy.com} ; Thu, 17 Aug 2006 01:48:39 -0500 } 9, 20 -- Received: (qmail 65219 invoked by uid 60001); 17 Aug 2006 06:48:39 -0000 } 9, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 9, 20 -- s=s1024; d=yahoo.com; } 9, 20 -- h=Message-ID:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; } 9, 20 -- b=46+tg4vtVqYk4X30p1FZiDj9JUtYOUZ70i9iC+IOR1ImeYHrIORqoXUOjWeXobDcVAU02OuMvDMZ4v7ppX2u88xgiQDYbIyQvQgXztDbAN583tchZWrl6Hg9mt/w7+WBRWOLe0BnTVxaZ/lKCbshR91+u4UvaKgZf7+dhg1OGIU= ; } 9, 20 -- Message-ID: {20060817064839.65217.qmail-at-web37408.mail.mud.yahoo.com} } 9, 20 -- Received: from [80.122.101.102] by web37408.mail.mud.yahoo.com via HTTP; Wed, 16 Aug 2006 23:48:39 PDT } 9, 20 -- Date: Wed, 16 Aug 2006 23:48:39 -0700 (PDT) } 9, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 9, 20 -- Subject: Re: [Microscopy] need infos about e diffraction in TEM } 9, 20 -- To: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} } 9, 20 -- Cc: microscopy-at-microscopy.com } 9, 20 -- In-Reply-To: {200608141344.k7EDiSc09038-at-www.matscieng.sunysb.edu} } 9, 20 -- MIME-Version: 1.0 } 9, 20 -- Content-Type: text/plain; charset=iso-8859-1 } 9, 20 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
--
Andy Buckley
AB78-at-ESC.CAM.AC.UK DEPARTMENT OF EARTH SCIENCES UNIVERSITY OF CAMBRIDGE Tel +44 1223 333469 DOWNING STREET +44 1223 333400 CAMBRIDGE FAX +44 1223 333450 CB2 3EQ
Manager of XRD discussion list: http://www.jiscmail.ac.uk/lists/xrd.html
==============================Original Headers============================== 10, 22 -- From ab78-at-esc.cam.ac.uk Fri Aug 18 04:04:14 2006 10, 22 -- Received: from rock.esc.cam.ac.uk (rock.esc.cam.ac.uk [131.111.41.250]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7I94EdF025536 10, 22 -- for {microscopy-at-microscopy.com} ; Fri, 18 Aug 2006 04:04:14 -0500 10, 22 -- Received: from andyb.esc.cam.ac.uk ([192.168.17.155]) 10, 22 -- by rock.esc.cam.ac.uk with esmtps (TLSv1:AES256-SHA:256) 10, 22 -- (Exim 4.60) 10, 22 -- (envelope-from {ab78-at-esc.cam.ac.uk} ) 10, 22 -- id 1GE0Gv-0007Xu-UX; Fri, 18 Aug 2006 10:04:13 +0100 10, 22 -- Message-ID: {44E5824A.8000803-at-esc.cam.ac.uk} 10, 22 -- Date: Fri, 18 Aug 2006 10:03:06 +0100 10, 22 -- From: Andy Buckley {ab78-at-esc.cam.ac.uk} 10, 22 -- Reply-To: ab78-at-esc.cam.ac.uk 10, 22 -- User-Agent: Thunderbird 1.5.0.5 (Windows/20060719) 10, 22 -- MIME-Version: 1.0 10, 22 -- To: nizets2-at-yahoo.com, microscopy-at-microscopy.com, 10, 22 -- jquinn-at-www.matscieng.sunysb.edu 10, 22 -- Subject: Re: [Microscopy] Re: need infos about e diffraction in TEM 10, 22 -- References: {200608170654.k7H6sQM9026028-at-ns.microscopy.com} 10, 22 -- In-Reply-To: {200608170654.k7H6sQM9026028-at-ns.microscopy.com} 10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I was able to obtain a reprint of ELECTRON MICROSCOPY OF THIN CRYSTALS by Hirsch, et al. from Krieger Publishing Company, Malabar, Florida
Cheers
Roger A. Ristau, PhD Electron Microscopy Specialist Institute of Materials Science 97 North Eagleville Road University of Connecticut Storrs, CT 06269 vox: 860-486-5379 fax: 860-486-4745
} From: nizets2-at-yahoo.com } Reply-To: nizets2-at-yahoo.com } Date: Thu, 17 Aug 2006 01:57:38 -0500 } To: raristau-at-ims.uconn.edu } Subject: [Microscopy] Re: need infos about e diffraction in TEM } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Thanks for the tip, but this book is not available at } Amazon. I did a search on the www but I cannot find a } distributor (it is probably too old). } } I already spent one day on the link given by Sousan } (very nice). } } Would there be another good reference book (more } recent perhaps) which does the same job as Hirsch's? } } Regards, } } Stephane } } --- Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} wrote: } } } Stephane } } } } Purchase a copy of Hirsch's book. } } Go to Amazon.com. } } Search for "transmission electron hirsch". } } } } regards, } } } } Jim } } } } } From mail-at-ns.microscopy.com Mon Aug 14 07:30:49 } } 2006 } } } Date: Mon, 14 Aug 2006 06:33:44 -0500 } } } To: jquinn-at-www.matscieng.sunysb.edu } } } From: nizets2-at-yahoo.com } } } Reply-to: nizets2-at-yahoo.com } } } X-Resent-From: "Microscopy Listserver" } } {microscopy-at-microscopy.com} } } } Subject: [Microscopy] need infos about e } } diffraction in TEM } } } Errors-To: } } MicroscopyListSpamFilter-at-microscopy.com } } } X-lewp: MicroscopyListSpam NAGS } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The } } Microscopy Society of America } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ---------------------------------------------------------------------------- } } } } } } Dear listers, } } } } } } Although I understand the principle behind } } electron } } } diffraction in TEM I have no idea about the kind } } of } } } informations this technique gives and how to } } interpret } } } the diffraction pattern. } } } If you could give me a www address which explains } } that } } } I would be much grateful. } } } } } } Now a very practical problem could be perhaps } } used as } } } an example: we have a mixture of aluminosilicate } } } mineral (I have cut 70 nm sections) with approx. } } 90% } } } of mordenite and 10% quarz. } } } 1) Can I use e diffraction to distinguish both } } types } } } of particles? (to verify the purity of the } } powder) } } } 2) What kind of information about the cristal } } } structure can e diffraction give me in this case? } } } 3) Can I detect a change -and which change- to } } the } } } cristal structure using this technique if the } } mineral } } } is heat and treated with strong acids? (which } } actually } } } modifies the structure) } } } } } } We have also a tilt stage for tomography. Can } } this } } } bring further informations? } } } } } } Regards, } } } } } } Stephane } } } } } } } } } } } __________________________________________________ } } } Do You Yahoo!? } } } Tired of spam? Yahoo! Mail has the best spam } } protection around } } } http://mail.yahoo.com } } } } } } ==============================Original } } Headers============================== } } } 8, 18 -- From nizets2-at-yahoo.com Mon Aug 14 } } 06:30:17 2006 } } } 8, 18 -- Received: from } } web37406.mail.mud.yahoo.com } } (web37406.mail.mud.yahoo.com [209.191.91.138]) } } } 8, 18 -- by ns.microscopy.com } } (8.12.11.20060308/8.12.8) with SMTP id } } k7EBUGao010591 } } } 8, 18 -- for {microscopy-at-microscopy.com} ; Mon, } } 14 Aug 2006 06:30:17 -0500 } } } 8, 18 -- Received: (qmail 81606 invoked by uid } } 60001); 14 Aug 2006 11:30:15 -0000 } } } 8, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; } } c=nofws; } } } 8, 18 -- s=s1024; d=yahoo.com; } } } 8, 18 -- } } } h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-T } ransfer-Encoding; } } } 8, 18 -- } } } b=rlmt7902ZNRzFTTFfQF7ruMzaWPunDe6Tu9YxDJ2pD9W3I+BsUwz4JFylLSb1NbV5Y1qFqa1jK6Y } E5BUUAa9EVhASSCrla6W/fKuGhTPt6o7J7paQwTF6sqlqLCbo9YrFe2LwGUS/eGKK8kO3I8ZVczxOM } yA1VvnaLJ+c9MHHSw= } } ; } } } 8, 18 -- Message-ID: } } } {20060814113015.81604.qmail-at-web37406.mail.mud.yahoo.com} } } } 8, 18 -- Received: from [80.122.101.102] by } } web37406.mail.mud.yahoo.com via HTTP; Mon, 14 Aug } } 2006 04:30:15 PDT } } } 8, 18 -- Date: Mon, 14 Aug 2006 04:30:15 -0700 } } (PDT) } } } 8, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} } } } 8, 18 -- Subject: need infos about e diffraction } } in TEM } } } 8, 18 -- To: microscopy-at-microscopy.com } } } 8, 18 -- MIME-Version: 1.0 } } } 8, 18 -- Content-Type: text/plain; } } charset=iso-8859-1 } } } 8, 18 -- Content-Transfer-Encoding: 8bit } } } ==============================End of - } } Headers============================== } } } } } } } } __________________________________________________ } Do You Yahoo!? } Tired of spam? Yahoo! Mail has the best spam protection around } http://mail.yahoo.com } } ==============================Original Headers============================== } 9, 20 -- From nizets2-at-yahoo.com Thu Aug 17 01:48:40 2006 } 9, 20 -- Received: from web37408.mail.mud.yahoo.com } (web37408.mail.mud.yahoo.com [209.191.91.140]) } 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } k7H6mdnF020219 } 9, 20 -- for {microscopy-at-microscopy.com} ; Thu, 17 Aug 2006 01:48:39 -0500 } 9, 20 -- Received: (qmail 65219 invoked by uid 60001); 17 Aug 2006 06:48:39 } -0000 } 9, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 9, 20 -- s=s1024; d=yahoo.com; } 9, 20 -- } h=Message-ID:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content } -Type:Content-Transfer-Encoding; } 9, 20 -- } b=46+tg4vtVqYk4X30p1FZiDj9JUtYOUZ70i9iC+IOR1ImeYHrIORqoXUOjWeXobDcVAU02OuMvDMZ } 4v7ppX2u88xgiQDYbIyQvQgXztDbAN583tchZWrl6Hg9mt/w7+WBRWOLe0BnTVxaZ/lKCbshR91+u4 } UvaKgZf7+dhg1OGIU= ; } 9, 20 -- Message-ID: {20060817064839.65217.qmail-at-web37408.mail.mud.yahoo.com} } 9, 20 -- Received: from [80.122.101.102] by web37408.mail.mud.yahoo.com via } HTTP; Wed, 16 Aug 2006 23:48:39 PDT } 9, 20 -- Date: Wed, 16 Aug 2006 23:48:39 -0700 (PDT) } 9, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 9, 20 -- Subject: Re: [Microscopy] need infos about e diffraction in TEM } 9, 20 -- To: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} } 9, 20 -- Cc: microscopy-at-microscopy.com } 9, 20 -- In-Reply-To: {200608141344.k7EDiSc09038-at-www.matscieng.sunysb.edu} } 9, 20 -- MIME-Version: 1.0 } 9, 20 -- Content-Type: text/plain; charset=iso-8859-1 } 9, 20 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 20 -- From raristau-at-ims.uconn.edu Fri Aug 18 09:02:32 2006 7, 20 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7IE2T5i008464 7, 20 -- for {microscopy-at-microscopy.com} ; Fri, 18 Aug 2006 09:02:31 -0500 7, 20 -- Received: from [137.99.20.210] (d20h210.public.uconn.edu [137.99.20.210]) 7, 20 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id k7IE2NSh029812; 7, 20 -- Fri, 18 Aug 2006 10:02:23 -0400 7, 20 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 7, 20 -- Date: Fri, 18 Aug 2006 10:01:55 -0400 7, 20 -- Subject: Re: [Microscopy] Re: need infos about e diffraction in TEM 7, 20 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 7, 20 -- To: {nizets2-at-yahoo.com} , {microscopy-at-microscopy.com} 7, 20 -- Message-ID: {C10B4093.13EF%raristau-at-ims.uconn.edu} 7, 20 -- In-Reply-To: {200608170657.k7H6vcJb028789-at-ns.microscopy.com} 7, 20 -- Mime-version: 1.0 7, 20 -- Content-type: text/plain; charset="US-ASCII" 7, 20 -- Content-transfer-encoding: 7bit 7, 20 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information. 7, 20 -- X-UConn-MailScanner: Found to be clean 7, 20 -- X-UConn-MailScanner-SpamCheck: ==============================End of - Headers==============================
We are looking for solid state secondary electron detector that is similar to Si PIN type, but more sensitive (higher gain) than Si (not scintillator type (E-T type).
Joe Wang
Project Manager Hermes Microvision, Inc. 1595 McVandless Drive Milpitas, CA 95035 e-Mail: joe.wang-at-hermes-microvision.com
Office: (408)273-5855
==============================Original Headers============================== 5, 24 -- From joe.wang-at-hermes-microvision.com Fri Aug 18 12:10:48 2006 5, 24 -- Received: from mail.hermes-microvision.com (mail.hermes-microvision.com [65.244.115.229]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7IHAk8M021826 5, 24 -- for {microscopy-at-microscopy.com} ; Fri, 18 Aug 2006 12:10:47 -0500 5, 24 -- Received: from hmilap108 ([10.200.98.18]) 5, 24 -- (authenticated bits=0) 5, 24 -- by mail.hermes-microvision.com (8.12.11/8.12.11) with ESMTP id k7IHAeaa009721 5, 24 -- for {microscopy-at-microscopy.com} ; Fri, 18 Aug 2006 10:10:43 -0700 5, 24 -- Message-Id: {200608181710.k7IHAeaa009721-at-mail.hermes-microvision.com} 5, 24 -- From: "Joe Wang" {joe.wang-at-hermes-microvision.com} 5, 24 -- To: {microscopy-at-microscopy.com} 5, 24 -- Subject: Secondary electron detector 5, 24 -- Date: Fri, 18 Aug 2006 10:07:56 -0700 5, 24 -- Organization: HMI 5, 24 -- MIME-Version: 1.0 5, 24 -- Content-Type: text/plain; 5, 24 -- charset="us-ascii" 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353 5, 24 -- Thread-Index: AcbC6Nmt/yFpA4h5QTyYYNWtr4lquQ== 5, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 5, 24 -- X-Hermes-Microvision.-MailScanner-Information: Scanned at Hermes Microvision for your protection 5, 24 -- X-Hermes-Microvision.-MailScanner: Found to be clean 5, 24 -- X-MailScanner-From: joe.wang-at-hermes-microvision.com ==============================End of - Headers==============================
For a few days I have been trying to post a message that we are giving away our JEOL 100CX, but I guess it was always filtered as SPAM. So here it is again:
We are giving away our JEOL 100CX STEM. It is in running condition (or I should say was until about 9 months ago, we haven't used it since). We will keep the chiller. The new owner will be responsible for packing and shipping. The STEM is located in Lakewood, Colorado. No warranties or guarantees.
Let me know if you are interested. I'll make a decision next week.
Michael Bode, Ph.D.
General Manager
OLYMPUS SOFT IMAGING SOLUTIONS 12596 West Bayaud Ave #300 Lakewood, CO 80228 USA Tel.: +1 (303) 234-9270 Fax.: +1 (303) 234-9271 E-mail: Mike.Bode-at-olympus-sis.com www.olympus-sis.com
==============================Original Headers============================== 10, 23 -- From Mike.Bode-at-olympus-sis.com Fri Aug 18 17:17:57 2006 10, 23 -- Received: from mail.soft-imaging.de (ns.soft-imaging.de [62.180.61.130]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7IMHu4O005936 10, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Aug 2006 17:17:56 -0500 10, 23 -- Received: from muenster.soft-imaging.net (muenster.soft-imaging.de [10.26.20.9]) 10, 23 -- by mail.soft-imaging.de (8.13.3/8.13.3/SuSE Linux 0.7) with ESMTP id k7IMI5fV012729 10, 23 -- for {Microscopy-at-microscopy.com} ; Sat, 19 Aug 2006 00:18:07 +0200 10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 23 -- Content-class: urn:content-classes:message 10, 23 -- MIME-Version: 1.0 10, 23 -- Content-Type: text/plain; 10, 23 -- charset="us-ascii" 10, 23 -- Subject: FW: Anybody interested in a JEOL 100CX 10, 23 -- Date: Sat, 19 Aug 2006 00:15:55 +0200 10, 23 -- Message-ID: {78B53BA1C5A2D9449EDA30A98800EC9442B0F0-at-ms-s-gws.soft-imaging.net} 10, 23 -- X-MS-Has-Attach: 10, 23 -- X-MS-TNEF-Correlator: 10, 23 -- Thread-Topic: Anybody interested in a JEOL 100CX 10, 23 -- Thread-Index: AcbDEmrptU3OlfipRl+LPzeOmwR//gAAWgPQ 10, 23 -- From: "Mike Bode" {Mike.Bode-at-olympus-sis.com} 10, 23 -- To: {Microscopy-at-microscopy.com} 10, 23 -- Content-Transfer-Encoding: 8bit 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k7IMHu4O005936 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both zzhang-at-uwyo.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: zzhang-at-uwyo.edu Name: Zhaojie Zhang
Organization: University of Wyoming
Title-Subject: [Filtered] Question-Immuno-gold test
Question: Dear Listers:
I am having a problem with the secondary antibody - Immuno-gold conjugate (6 nm from Electron Microscopy Sciences). Here is what I did -
I run a test with a LR White embeded sample following the "standard" protocol for immuno-gold labeling. I could not find any gold particles - no labeling, no background. Trying to figure out what is wrong, I tested the secondary antibody alone - put a small drop of the original antibody (no dilution) directly onto a TEM grid, air dry and viewed with TEM. I could not find any 6 nm gold particles. I did find a few particles ranging from 50 nm to 150 nm.
I then called the company, explained my problem. I was told that that is NOT the way I should test the secondary antibody, instead, I should do a dot-spot test (with silver enhancement?)
My Question is: Has anyone had this problem before? How do you check if the secondary antibody (immuno-gold) works or not?
Thank you,
Zhaojie Zhang Microscopy Core Facility University of Wyoming
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Email: KLRadke-at-ucdavis.edu Name: Kathryn Radke
Organization: Univ. California, Davis
Title-Subject: [Filtered] LM - coupling digital camera back to Nikon Diaphot
Question: I'd like to use my Nikon D70s camera back on my lab's 1985 Nikon Diaphot inverted cell culture microscope. The Diaphot front port has a fitting that couples to Nikon film backs (e.g. FE2, FTN) having Nikon F bayonet type lens mount. When the microscope is set up properly, the image viewed through the eyepieces focused on the film.
The D70S back won't fit directly onto the Diaphot. A local camera store tells me that I could use a T mount adapter that is designed to couple a digital back with a telescope. With the T mount adapter in place, the digital back can be used in Manual mode and the shutter speed can be set using the "command dial" on the camera back. I would also need to purchase a remote cord to operate the back without shaking the microscope.
I would be most grateful if anyone can give some feedback about necessary parts, operation, etc. Are there websites that have relevant information?
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Judy Murphy, PhD Microscopy Training, Imaging, and Lab Design Stockton, CA 95219 murphyjudy-at-comcast.net
connie.a.cummings-at-gsk.com wrote: Organization: GSK --| --|Title-Subject: [Filtered] EM tech certification --| --|Question: I was wondering where do you get information on certification on EM certification. --|
==============================Original Headers============================== 10, 17 -- From murphyjudy-at-comcast.net Fri Aug 18 18:30:31 2006 10, 17 -- Received: from alnrmhc11.comcast.net (alnrmhc14.comcast.net [206.18.177.54]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7INUVZY024544 10, 17 -- for {microscopy-at-microscopy.com} ; Fri, 18 Aug 2006 18:30:31 -0500 10, 17 -- Received: from [192.168.1.106] (c-67-181-78-13.hsd1.ca.comcast.net[67.181.78.13]) 10, 17 -- by comcast.net (alnrmhc14) with ESMTP 10, 17 -- id {20060818233030b1400gl0sve} ; Fri, 18 Aug 2006 23:30:31 +0000 10, 17 -- Message-ID: {44E64D94.1030204-at-comcast.net} 10, 17 -- Date: Fri, 18 Aug 2006 16:30:28 -0700 10, 17 -- From: Judy Murphy {murphyjudy-at-comcast.net} 10, 17 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 10, 17 -- X-Accept-Language: en-us, en 10, 17 -- MIME-Version: 1.0 10, 17 -- To: microscopy-at-microscopy.com 10, 17 -- Subject: Re: viaWWW: EM tech certification 10, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed 10, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
There are two tests you can do when you don't get a positive result im immuno gold labelling and when you want to check whether the conjugate performs up to standards:
1. An activity test, using a dot-spot system In this test a dilution series of corresponding IgG (Rabbit IgG in the present case) is spotted on a strip of nitrocellulose, and after blocking the strip is incubated with the gold conjugate. Silver enhancement is only required if you would test an ultra small particle conjugate in which the gold does not significantly contribute to otain colored dots. A 6nm conjugate has 'sufficient color' and does not need enhancement. We can supply you with a procedure if you like, they are described extensively in our Newsletter 4. 2. A TEM test in which the gold conjugate is adsorbed (not dried from the stock solution) onto a grid that is filmed and coated with poly-L- lysine which by its positive charge will bind negatively charged gold particles. Grids are washed on distilled water after adsorption. Again, we can supply you with a procedure if you like.
Drying a small drop of undiluted conjugated onto a filmed grid makes it not easy, if not impossible, to see particles or to evaluate what particle sizes you have. After all there is buffer components and protecting protein in the conjugate solution that all dry onto the grid. Also, upon drying particles tend to aggregate and form clumps (that are usually not easy to dissolve completely again, so they are pretty solid) and which will give erroneous readings.
I will be happy to help trying to stablish whether antigens may have been damaged preventing positive results or whether the primary antibody or secondary antibody have lost activity.
Cheers, hope this helps, get in touch if you like. New Zealand is still good fun!!
Jan Leunissen
Aurion - President Electron Microscopy Research Advisor Costerweg 5 Dept Anatomy and Structural Biology 6702 A Wageningen Otago School of Medicine The Netherlands Dunedin, New Zealand phone 31-317-497676 phone 64-3-4795465 fax 31-317-415955 fax 64-3-4797254 http://www.aurion.nl http://anatomy.otago.ac.nz
==============================Original Headers============================== 10, 20 -- From leunissen-at-aurion.nl Fri Aug 18 18:43:14 2006 10, 20 -- Received: from fep06.xtra.co.nz (fep06.xtra.co.nz [210.54.141.240]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7INhDl6002485 10, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Aug 2006 18:43:14 -0500 10, 20 -- Received: from [192.168.1.50] (really [210.86.106.215]) by fep06.xtra.co.nz 10, 20 -- with ESMTP 10, 20 -- id {20060818234311.GTFE16670.fep06.xtra.co.nz-at-[192.168.1.50]} ; 10, 20 -- Sat, 19 Aug 2006 11:43:11 +1200 10, 20 -- In-Reply-To: {200608182305.k7IN5j1F017177-at-ns.microscopy.com} 10, 20 -- References: {200608182305.k7IN5j1F017177-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 (Apple Message framework v752.2) 10, 20 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 10, 20 -- Message-Id: {86E9F1BD-5415-4582-AD4D-201906E99C1A-at-aurion.nl} 10, 20 -- Cc: Microscopy-at-microscopy.com 10, 20 -- Content-Transfer-Encoding: 7bit 10, 20 -- From: Jan Leunissen {leunissen-at-aurion.nl} 10, 20 -- Subject: Re: [Microscopy] viaWWW: Question-Immuno-gold test 10, 20 -- Date: Sat, 19 Aug 2006 11:43:10 +1200 10, 20 -- To: zzhang-at-uwyo.edu 10, 20 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
I have a new topic that is not particularly relevant today but probably will be in the foreseeable future.
The newer SEMs are very advanced and make maximum use of PC interfacing. The PCs will age but should work OK or be replaced by faster models if necessary or due to supportability factors.
What about the SEM itself? These are now made with surface mount technology, flip chip BGAs and FPGAs.
Some makers are saying that they will support a specific product line for 5-10 more years, or less. After that, one needs to buy a new SEM. This is what they are saying....not all SEM makers. But enough to trigger the sensitivity of a potentially big bow wave of problems. The up-front cost of a tool really does not put it in the disposable category. Plus, in an industrial setting, how would depreciation factor in? But if the tool cannot be supported after say ten years, then it a disposable item if there are no other service resources. The dearth of schematics is a huge issue with me since I cannot talk to the service folks with knowledge and also impacts the future cut off of service. One is basically buying a $500K boat anchor that needs replacing every ten year or so. Yep, sounds like a boat problem.
Recall that the Amray and early JEOL tools were relatively easy to fix... electronics-wise. These newer systems are not at all easy to fix or troubleshoot.
I'm just blowing the whistle on what I think is a pending huge problem.
Alternate opinions?
gary g.
==============================Original Headers============================== 11, 17 -- From gary-at-gaugler.com Fri Aug 18 23:06:32 2006 11, 17 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k7J46Vmm017237 11, 17 -- for {microscopy-at-microscopy.com} ; Fri, 18 Aug 2006 23:06:31 -0500 11, 17 -- Received: (qmail 17545 invoked from network); 18 Aug 2006 21:06:31 -0700 11, 17 -- Received: by simscan 1.1.0 ppid: 17542, pid: 17543, t: 0.1494s 11, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 17 -- by qsmtp2 with SMTP; 18 Aug 2006 21:06:30 -0700 11, 17 -- Message-Id: {7.0.1.0.2.20060818204828.0247a458-at-gaugler.com} 11, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 11, 17 -- Date: Fri, 18 Aug 2006 21:06:33 -0700 11, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 11, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 17 -- Subject: New subject-service life of new SEMs 11, 17 -- Mime-Version: 1.0 11, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
All: Abebooks.com had several copies of both Hirsch books available, found by Advanced search on--- Author: hirsch Keywords: electron microscopy
-mike reedy .
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-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
Gary, As a co-owner of a Jeol 6100 with an intermittent problem, and a soon-to-be owner of an 840F, I could not agree more. My 6100 does have schematics, but at the board level they are not even the correct boards. In defense of the manufacturers, they have high development costs and very low unit sales. Plus, the market size is very limited. If they were making a big profit, many other competitors would jump in.
I wonder why there are not specialty shops that take existing SEMs, strip them down to the column and other mechanical hardware, and couple up wholly new electronics. (Maybe there are. If so, I'd like to hear about them.) Isn't it just a more (ok, way more) complicated version of taking an old loudspeaker and hooking up a new amplifier?
With the technical service info secret, the customer is really leasing the tool, not buying it, since the customer is entirely dependent on the manufacturer to keep it running, and the manufacturer can define its useful life.
Is there a SEM manufacturer who sells complete service literature?
Bernard Cuzzillo Berkeley, CA USA
==============================Original Headers============================== 5, 14 -- From bernard-at-bearinc.com Sat Aug 19 00:59:21 2006 5, 14 -- Received: from bearinc.com (bearinc.com [64.62.217.44]) 5, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k7J5xKvU006610 5, 14 -- for {Microscopy-at-microscopy.com} ; Sat, 19 Aug 2006 00:59:21 -0500 5, 14 -- Received: from 24.7.101.58 ([24.7.101.58]) by bearinc.com for {Microscopy-at-microscopy.com} ; Fri, 18 Aug 2006 22:59:20 -0700 5, 14 -- Message-ID: {44E6A8B3.6030407-at-bearinc.com} 5, 14 -- Date: Fri, 18 Aug 2006 22:59:15 -0700 5, 14 -- From: Bernard Cuzzillo {bernard-at-bearinc.com} 5, 14 -- User-Agent: Thunderbird 1.5.0.5 (Windows/20060719) 5, 14 -- MIME-Version: 1.0 5, 14 -- To: Microscopy-at-microscopy.com 5, 14 -- Subject: Service life of new SEMs -- Agreed! 5, 14 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 14 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
As one of the guys who can't afford the 500k for a new unit, I am looking forward to all of you dumping out of your 10 year old units when the time comes. My solution is simple, if the price is cheap enough you buy several for spares. Then you can remove-and-replace as required. I am currently keeping an old Kevex system running this way and have backup boards purchased for nothing on Ebay. I have also just purchased a complete JEOL 6100F for 800 bucks and am keeping an eye out for another. As a hobbyist this is my only choice so the less service the better for me.
On the other side of the coin, I would be blowing a fuse knowing my half mill investment would be worthless in 10 years. I agree with Gary that the schematics make all the difference.
Tom Kaye
==============================Original Headers============================== 5, 21 -- From tom-at-tomkaye.com Sat Aug 19 01:18:00 2006 5, 21 -- Received: from tomkaye.com (mxgateway.techpro.com [64.4.207.8]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7J6HxT6017057 5, 21 -- for {Microscopy-at-microscopy.com} ; Sat, 19 Aug 2006 01:18:00 -0500 5, 21 -- Received: from TKLAPTOP [65.39.118.10] by tomkaye.com with ESMTP 5, 21 -- (SMTPD32-8.14) id AD14220A00F4; Sat, 19 Aug 2006 01:17:56 -0500 5, 21 -- From: "Tom" {tom-at-tomkaye.com} 5, 21 -- To: {Microscopy-at-microscopy.com} 5, 21 -- Subject: RE: [Microscopy] Service life of new SEMs -- Agreed! 5, 21 -- Date: Sat, 19 Aug 2006 01:17:59 -0500 5, 21 -- Message-ID: {LHECKJAKKIOGKDBHHJMLMEDKCLAA.tom-at-tomkaye.com} 5, 21 -- MIME-Version: 1.0 5, 21 -- Content-Type: text/plain; 5, 21 -- charset="iso-8859-1" 5, 21 -- Content-Transfer-Encoding: 7bit 5, 21 -- X-Priority: 3 (Normal) 5, 21 -- X-MSMail-Priority: Normal 5, 21 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0) 5, 21 -- In-reply-to: {200608190604.k7J646Pb014317-at-ns.microscopy.com} 5, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1165 5, 21 -- Importance: Normal ==============================End of - Headers==============================
The chair of the Microscopy Society of America Certification Board is E. Ann Ellis at eann.ellis-at-worldnet.att.com
Good Luck!
connie.a.cummings-at-gsk.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both connie.a.cummings-at-gsk.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: connie.a.cummings-at-gsk.com } Name: Connie Cummings } } Organization: GSK } } Title-Subject: [Filtered] EM tech certification } } Question: I was wondering where do you get information on certification on EM certification. } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 12 -- From zaluzec-at-microscopy.com Fri Aug 18 18:06:15 2006 } 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7IN6FvF018864 } 6, 12 -- for {microscopy-at-microscopy.com} ; Fri, 18 Aug 2006 18:06:15 -0500 } 6, 12 -- Mime-Version: 1.0 } 6, 12 -- X-Sender: (Unverified) } 6, 12 -- Message-Id: {p06110402c10bf85b4848-at-[206.69.208.22]} } 6, 12 -- Date: Fri, 18 Aug 2006 18:06:13 -0500 } 6, 12 -- To: microscopy-at-microscopy.com } 6, 12 -- From: connie.a.cummings-at-gsk.com (by way of MicroscopyListserver) } 6, 12 -- Subject: viaWWW: EM tech certification } 6, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== } } }
==============================Original Headers============================== 4, 19 -- From randerson20-at-tampabay.rr.com Sat Aug 19 07:50:06 2006 4, 19 -- Received: from ms-smtp-05.tampabay.rr.com (ms-smtp-05.tampabay.rr.com [65.32.5.135]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7JCo6H7032338 4, 19 -- for {Microscopy-at-Microscopy.Com} ; Sat, 19 Aug 2006 07:50:06 -0500 4, 19 -- Received: from [127.0.0.1] (rrcs-24-73-73-214.se.biz.rr.com [24.73.73.214]) 4, 19 -- by ms-smtp-05.tampabay.rr.com (8.13.6/8.13.6) with ESMTP id k7JCo0WL023097; 4, 19 -- Sat, 19 Aug 2006 08:50:03 -0400 (EDT) 4, 19 -- Message-ID: {44E708F7.8070809-at-tampabay.rr.com} 4, 19 -- Date: Sat, 19 Aug 2006 08:49:59 -0400 4, 19 -- From: Ronald Anderson {randerson20-at-tampabay.rr.com} 4, 19 -- User-Agent: Thunderbird 1.5.0.5 (Windows/20060719) 4, 19 -- MIME-Version: 1.0 4, 19 -- To: connie.a.cummings-at-gsk.com, Listserver {Microscopy-at-Microscopy.Com} 4, 19 -- Subject: Re: [Microscopy] viaWWW: EM tech certification 4, 19 -- References: {200608182306.k7IN6PG5019414-at-ns.microscopy.com} 4, 19 -- In-Reply-To: {200608182306.k7IN6PG5019414-at-ns.microscopy.com} 4, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 19 -- Content-Transfer-Encoding: 7bit 4, 19 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (classyjay247-at-yahoo.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, August 19, 2006 at 08:46:19 ---------------------------------------------------------------------------
Email: classyjay247-at-yahoo.com Name: Uchenna Victor
Organization: federal university of technology Owerri
Education: Undergraduate College
Location: Abuja Nigeria
Question: what are the transmission patterns of electron gun
Hirsch has also just listed at amazon.com along with several other diffraction books. Interesting tidbit: there are over 750 titles on electron microscopy on amazon!!! I was amazed. Below are a few with links on diffraction that one lister asked about. Cheers, Judy
Judy Murphy, PhD Microscopy Training, Imaging, and Lab Design Stockton, CA 95219 murphyjudy-at-comcast.net
Hirsch EM of Thin Crystals http://s1.amazon.com/exec/varzea/ts/exchange-glance/Y04Y5461265Y9057909/002-0880518-7016064
Modern Crystallography http://www.amazon.com/exec/obidos/ASIN/0387100520/002-0880518-7016064
Diffraction (Student Micrograph in Physics) http://www.amazon.com/exec/obidos/ASIN/0852745796/002-0880518-7016064
Andrews Interpretation of Electron Diffraction Patterns http://www.amazon.com/exec/obidos/ASIN/0306305348/002-0880518-7016064
McCall Interpretive Techniques for Microstructural Analysis http://www.amazon.com/exec/obidos/ASIN/0306310368/002-0880518-7016064
Rostoker Interpretation of Metallographic Structures http://www.amazon.com/exec/obidos/ASIN/0125982550/002-0880518-7016064
RMS Series Intro to Crystallography http://www.amazon.com/exec/obidos/ASIN/0198564333/002-0880518-7016064
Transmission Electron Microscopy of Minerals and Rocks http://www.amazon.com/exec/obidos/ASIN/0521350980/002-0880518-7016064
mike.reedy-at-cellbio.duke.edu wrote:
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==============================Original Headers============================== 15, 19 -- From murphyjudy-at-comcast.net Sat Aug 19 12:19:15 2006 15, 19 -- Received: from rwcrmhc15.comcast.net (rwcrmhc15.comcast.net [204.127.192.85]) 15, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7JHJFnF030017 15, 19 -- for {Microscopy-at-microscopy.com} ; Sat, 19 Aug 2006 12:19:15 -0500 15, 19 -- Received: from [192.168.1.106] (c-67-181-78-13.hsd1.ca.comcast.net[67.181.78.13]) 15, 19 -- by comcast.net (rwcrmhc15) with ESMTP 15, 19 -- id {20060819171913m1500jrcbte} ; Sat, 19 Aug 2006 17:19:14 +0000 15, 19 -- Message-ID: {44E74811.2040307-at-comcast.net} 15, 19 -- Date: Sat, 19 Aug 2006 10:19:13 -0700 15, 19 -- From: Judy Murphy {murphyjudy-at-comcast.net} 15, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 15, 19 -- X-Accept-Language: en-us, en 15, 19 -- MIME-Version: 1.0 15, 19 -- To: mike.reedy-at-cellbio.duke.edu, Microscopy {Microscopy-at-microscopy.com} 15, 19 -- Subject: Re: [Microscopy] Re: need infos about e diffraction in TEM 15, 19 -- References: {200608190545.k7J5jNtW000349-at-ns.microscopy.com} 15, 19 -- In-Reply-To: {200608190545.k7J5jNtW000349-at-ns.microscopy.com} 15, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
You are right when you say that these are not disposable items. If manufacturers have the attitude that we in the academic world can simply conjure up $250-500,000 because they determine that the lifespan of their EM is 10 years, then the EM community collectively should send a strong message to the contrary. My attitude is this: if an EM manufacturer will not continue to support and keep my EM going until I am ready (and able) to replace it, then (when I do find the funds for a replacement) my next microscope will definitely not be one of theirs. There are companies that apparently stand up for their microscopes for the extended lifetimes they should be proud of. As an example, the lab I was trained in still has the microscope that I was trained on some 30 years ago (they added a digital camera, and I'm told it's one of the favored TEMs in the lab). --Jan Factor
--------------------------------------- Jan Robert Factor, Ph.D. Professor of Biology --------------------------------------- Natural Sciences Purchase College State University of New York 735 Anderson Hill Rd. Purchase, NY 10577 USA --------------------------------------- Office Tel: 914-251-6659 Office Fax: 914-251-6635 E-mail: jfactor-at-ns.purchase.edu or- jan.factor-at-purchase.edu ---------------------------------------
gary-at-gaugler.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Listers: } } I have a new topic that is not particularly relevant } today but probably will be in the foreseeable future. } } The newer SEMs are very advanced and make maximum use } of PC interfacing. The PCs will age but should work OK } or be replaced by faster models if necessary or due to } supportability factors. } } What about the SEM itself? These are now made with } surface mount technology, flip chip BGAs and FPGAs. } } Some makers are saying that they will support a specific } product line for 5-10 more years, or less. After that, one needs } to buy a new SEM. This is what they are saying....not } all SEM makers. But enough to trigger the sensitivity of } a potentially big bow wave of problems. The up-front cost } of a tool really does not put it in the disposable category. } Plus, in an industrial setting, how would depreciation factor in? } But if the tool cannot be supported after say ten years, then } it a disposable item if there are no other service resources. } The dearth of schematics is a huge issue with me since I cannot } talk to the service folks with knowledge and also impacts the } future cut off of service. One is basically buying a $500K } boat anchor that needs replacing every ten year or so. Yep, } sounds like a boat problem. } } Recall that the Amray and early JEOL tools were relatively } easy to fix... electronics-wise. These newer systems are not } at all easy to fix or troubleshoot. } } I'm just blowing the whistle on what I think is a pending } huge problem. } } Alternate opinions? } } gary g. } } } } ==============================Original Headers============================== } 11, 17 -- From gary-at-gaugler.com Fri Aug 18 23:06:32 2006 } 11, 17 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) } 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k7J46Vmm017237 } 11, 17 -- for {microscopy-at-microscopy.com} ; Fri, 18 Aug 2006 23:06:31 -0500 } 11, 17 -- Received: (qmail 17545 invoked from network); 18 Aug 2006 21:06:31 -0700 } 11, 17 -- Received: by simscan 1.1.0 ppid: 17542, pid: 17543, t: 0.1494s } 11, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 } 11, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) } 11, 17 -- by qsmtp2 with SMTP; 18 Aug 2006 21:06:30 -0700 } 11, 17 -- Message-Id: {7.0.1.0.2.20060818204828.0247a458-at-gaugler.com} } 11, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 } 11, 17 -- Date: Fri, 18 Aug 2006 21:06:33 -0700 } 11, 17 -- To: MSA listserver {microscopy-at-microscopy.com} } 11, 17 -- From: Gary Gaugler {gary-at-gaugler.com} } 11, 17 -- Subject: New subject-service life of new SEMs } 11, 17 -- Mime-Version: 1.0 } 11, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } ==============================End of - Headers============================== } } }
==============================Original Headers============================== 6, 20 -- From jfactor-at-ns.purchase.edu Sat Aug 19 16:53:27 2006 6, 20 -- Received: from mta1.srv.hcvlny.cv.net (mta1.srv.hcvlny.cv.net [167.206.4.196]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7JLrRoe012401 6, 20 -- for {microscopy-at-msa.microscopy.com} ; Sat, 19 Aug 2006 16:53:27 -0500 6, 20 -- Received: from [127.0.0.1] (ool-4357ef27.dyn.optonline.net [67.87.239.39]) 6, 20 -- by mta1.srv.hcvlny.cv.net 6, 20 -- (Sun Java System Messaging Server 6.2-6.01 (built Apr 3 2006)) 6, 20 -- with ESMTP id {0J4900GR5M6U8X40-at-mta1.srv.hcvlny.cv.net} for 6, 20 -- microscopy-at-msa.microscopy.com; Sat, 19 Aug 2006 17:54:35 -0400 (EDT) 6, 20 -- Date: Sat, 19 Aug 2006 17:53:39 -0400 6, 20 -- From: Jan Factor {jfactor-at-ns.purchase.edu} 6, 20 -- Subject: Re: [Microscopy] New subject-service life of new SEMs 6, 20 -- In-reply-to: {200608190407.k7J47bjo018742-at-ns.microscopy.com} 6, 20 -- To: gary-at-gaugler.com, Microscopy Listserver {microscopy-at-msa.microscopy.com} 6, 20 -- Message-id: {44E78863.4030605-at-ns.purchase.edu} 6, 20 -- MIME-version: 1.0 6, 20 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-transfer-encoding: 7BIT 6, 20 -- References: {200608190407.k7J47bjo018742-at-ns.microscopy.com} 6, 20 -- User-Agent: Thunderbird 1.5.0.5 (Windows/20060719) ==============================End of - Headers==============================
I agree as well. The problem is that the older systems were easier to maintain in major part due to the way they were made and the technology at that time--multiple pull out PC cards, socketed ICs, etc. That is all going away now as the makers use TSOP (small outline surface mount IC) ICs, chip resistors and capacitors and pack more functions (FPGAs) onto a single PC board. Result....fewer but more dense PC boards that are probably close to impossible or at best, difficult to repair. However, the other side of this more hands-off manufacturing is greater consistency. This results in fewer failures. The other detrimental factor though is the out-sourcing of portions of the system. The SEM makers build the frame, column and associated parts but have some other company do the electronics or portions of the electronics and/or software. In many cases, the field service folks do not have schematics that accurately reflect the machine they are working on--not to mention that they can no longer replace individual components. It is a replace the whole thing scenario now. The advent of advanced computer control seems to be to keep fixing bugs and adding new or different features to justify the software engineering department or source. Some of these software updates fix things that are so stupid and obvious it seems that software debugging is the job of the customer. That is very bad.
The Devil is in the details. The Devil here is dollars. If the systems are more reliable, people can keep them running for longer periods of time. Remember that most companies have two major cost centers--sales and service. So the sales side sells a new system which is then covered under a maintenance contract by the service side. With a reliable system, the service side makes a nice profit since there are fewer service calls than before. In the mean time, the sales side complains that they are not selling enough systems. The solution? Stop supporting fielded systems to force users to buy a new SEM.
Since I have no financial interest any more with KLA-Tencor I can use them as a good example of how I perceive the internal thinking (or lack thereof) is seen in action. I owned multiple Amray SEMs over the years and at first had Amray contracts. Then, when KLA bought out Amray, service moved to KLA. Over time, KLA began to drop service contracts for their thermionic SEMs. Since my last Amray SEM was an FE, I did not get dropped. Their support prices were quite reasonable compared to today's FESEM maintenance cost. Anyway, as they began dropping thermionic support they also started to lay off service personnel. An obvious cost cutting mentality. Then, as more Amray FE optics were put into KLA inspection tools, the Amray techs got pulled (sucked) into the tool side. Then, behold-- less support for Amray FE SEMs and less support for KLA tools since the techs left leaving a support vacuum. This was in 2003-2004. I suspect that since then, they have either stopped supporting FESEMs or soon will. That is too bad since the later systems were real work horses. I had very few problems with any of my Amray systems. But eventually, the performance just was not there. This was because Amray stopped developing new systems. Software development also stopped (it always was out-sourced). The current manufacturers are not in this non-developmental mode at all.
So the business model of continuous introduction of new models of SEMs and other systems is to "have a growth path" when in fact, earlier models do the intended job just fine, thank you. But they do need support from time to time. Agreed....some need a lot more support than others. But are they newer or older systems? Some are newer or newest, unfortunately. So the service side complains and the reaction from corporate is to cull their product line to simplify the field complement of systems. Optimal in their view is probably that every installation has the exact same system. This would reduce parts logistics, documentation deviations, software option switches, etc. Of course it would. But not all customers' needs are identical.
My gut feeling is--don't count on more than 7-10 years of support from the OEM. And there probably won't be second or third party options. Like I said initially--you are going to be stuck with a boat anchor. It is just a matter of time. This is true for older systems. But I think the time frame to become an anchor is getting shorter. I'm not sure that my crystal ball is any better than any one else's. But I came away from M&M 2006 with this gnawing uncomfortable feeling. Newer isn't always better or best. But in a competitive world, not producing newer may be fatal. If the OEM manufacturers don't survive, one certainly cannot get a service contract from them! It is a horrible business situation.
When buying a new system, you cannot count on determining how long they will support the system. The sales side will tell you whatever you want to hear. The only viable option IMO is to find out what and how old of systems they are currently supporting. Are the postings on this list for SEM/TEM support due to cost reduction at the customer side or lack of support options from the manufacturers? I don't know. That is a good question to ask.
It just seems to me that what has been rather common in the past is pretty quickly being obsoleted. This pertains to how systems are supported, for how long and by whom. But the business model seems to be the same. The issue or point of equipment saturation is quite valid. If all auto makers made cars that lasted 30 years, then who would be the volume new car buyers? As long as the car gets you from point A to point B and back, what is the advantage of a new car? The discriminator is gas mileage, features, etc. With SEM/TEM it is performance. As long as they do what is needed, a newer model might be nice but is not necessary to get the job done.
gary g.
At 02:53 PM 8/19/2006, you wrote: } You are right when you say that these are not disposable items. If } manufacturers have the attitude that we in the academic world can } simply conjure up $250-500,000 because they determine that the } lifespan of their EM is 10 years, then the EM community collectively } should send a strong message to the contrary. My attitude is this: } if an EM manufacturer will not continue to support and keep my EM } going until I am ready (and able) to replace it, then (when I do } find the funds for a } replacement) my next microscope will definitely not be one of } theirs. There are companies that apparently stand up for their } microscopes for the extended lifetimes they should be proud of. As } an example, the lab I was trained in still has the microscope that I } was trained on some 30 years ago (they added a digital camera, and } I'm told it's one of the favored TEMs in the lab). } --Jan Factor } } --------------------------------------- } Jan Robert Factor, Ph.D. } Professor of Biology } --------------------------------------- } Natural Sciences } Purchase College } State University of New York } 735 Anderson Hill Rd. } Purchase, NY 10577 } USA } --------------------------------------- } Office Tel: 914-251-6659 } Office Fax: 914-251-6635 } E-mail: jfactor-at-ns.purchase.edu } or- jan.factor-at-purchase.edu } --------------------------------------- } } } } gary-at-gaugler.com wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 13, 21 -- From gary-at-gaugler.com Sat Aug 19 19:36:32 2006 13, 21 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k7K0aWeh024692 13, 21 -- for {microscopy-at-microscopy.com} ; Sat, 19 Aug 2006 19:36:32 -0500 13, 21 -- Received: (qmail 31522 invoked from network); 19 Aug 2006 17:36:30 -0700 13, 21 -- Received: by simscan 1.1.0 ppid: 31519, pid: 31520, t: 0.2869s 13, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 13, 21 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 13, 21 -- by qsmtp1 with SMTP; 19 Aug 2006 17:36:30 -0700 13, 21 -- Message-Id: {7.0.1.0.2.20060819154105.02544cd0-at-gaugler.com} 13, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 13, 21 -- Date: Sat, 19 Aug 2006 17:36:33 -0700 13, 21 -- To: Jan Factor {jfactor-at-ns.purchase.edu} 13, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 13, 21 -- Subject: Re: [Microscopy] New subject-service life of new SEMs 13, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 13, 21 -- In-Reply-To: {44E78863.4030605-at-ns.purchase.edu} 13, 21 -- References: {200608190407.k7J47bjo018742-at-ns.microscopy.com} 13, 21 -- {44E78863.4030605-at-ns.purchase.edu} 13, 21 -- Mime-Version: 1.0 13, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Hi all, I have a friend who needs to make a montage of a large number of optical micrographs. He has about 500 images. Can anybody suggest a PC software package that is easy to use? He is currently using Photoshop. Freeware or something that can be paid for and downloaded would be especially useful. Thanks for any suggestions,
Jennifer Ray Sloppy Materials Science & Engineering, Ph.D. Candidate Pennsylvania State University
==============================Original Headers============================== 3, 17 -- From jds451-at-psu.edu Sun Aug 20 09:51:57 2006 3, 17 -- Received: from webmail19.cac.psu.edu (webmail19.cac.psu.edu [128.118.1.205]) 3, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7KEpvZI004541 3, 17 -- for {microscopy-at-microscopy.com} ; Sun, 20 Aug 2006 09:51:57 -0500 3, 17 -- Received: (from webmail-at-localhost) 3, 17 -- by webmail19.cac.psu.edu (8.9.3/8.9.0) id KAA20369; 3, 17 -- Sun, 20 Aug 2006 10:51:56 -0400 (EDT) 3, 17 -- Date: Sun, 20 Aug 2006 10:51:56 -0400 (EDT) 3, 17 -- Message-Id: {200608201451.KAA20369-at-webmail19.cac.psu.edu} 3, 17 -- To: microscopy-at-microscopy.com 3, 17 -- Subject: Software: Montage 3, 17 -- From: "Jen Sloppy" {jds451-at-psu.edu} 3, 17 -- X-Mailer: Penn State WebMail 3, 17 -- X-Sender: jds451 3, 17 -- X-Originating-IP: 207.255.156.167 3, 17 -- MIME-Version: 1.0 3, 17 -- Content-Type: text/plain ==============================End of - Headers==============================
I have used Panavue ImageAssembler (http://www.panavue.com/) with some success. You do have to load the images in row order. I've sometimes had to stitch the rows first then assemble the rows into the final image rather than assembling the whole image in one pass. ImageAssembler can use either automatic or manual registration for the stitching. The auto registration works reasonably well for image sets with reasonable overlap (20-25%) in the images and if the features are easily distinguishable and not too complex. (I suspect that it just does a cross-correlation.) It will do some image warping and brightness/contrast adjustment to better blend the photos in the stitching process.
The software is pretty easy to use and the price is reasonable ($64 for the standard edition and $129 for the professional edition). The professional version will handle larger images.
The software has worked for me. Your mileage may vary!
Usual disclaimer: I have no vested interest in the product or company.
Good Luck, Henk Colijn
At 10:55 AM 8/20/2006, jds451-at-psu.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hendrik O. Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility www.ceof.ohio-state.edu 040 Fontana Labs, 116 W. 19th Ave
==============================Original Headers============================== 13, 26 -- From colijn.1-at-osu.edu Sun Aug 20 20:19:13 2006 13, 26 -- Received: from ER6S1.ENG.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7L1JClO007521 13, 26 -- for {microscopy-at-microscopy.com} ; Sun, 20 Aug 2006 20:19:13 -0500 13, 26 -- Received: from CONVERSION-DAEMON.er6s1.eng.ohio-state.edu by 13, 26 -- er6s1.eng.ohio-state.edu (PMDF V6.2-1x11 #31056) 13, 26 -- id {01M689NUXPKGA8NPYQ-at-er6s1.eng.ohio-state.edu} for 13, 26 -- microscopy-at-microscopy.com; Sun, 20 Aug 2006 21:19:10 -0400 (EDT) 13, 26 -- Received: from HOC3.osu.edu 13, 26 -- (d149-67-33-174.col.wideopenwest.com [67.149.174.33]) 13, 26 -- by er6s1.eng.ohio-state.edu (PMDF V6.2-1x11 #31056) 13, 26 -- with ESMTPA id {01M689NU56XYA8MMTU-at-er6s1.eng.ohio-state.edu} ; Sun, 13, 26 -- 20 Aug 2006 21:19:10 -0400 (EDT) 13, 26 -- Date: Sun, 20 Aug 2006 21:18:00 -0400 13, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 13, 26 -- Subject: Re: [Microscopy] Software: Montage 13, 26 -- In-reply-to: {200608201455.k7KEtawS007511-at-ns.microscopy.com} 13, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 13, 26 -- To: jds451-at-psu.edu 13, 26 -- Cc: microscopy-at-microscopy.com 13, 26 -- Message-id: {7.0.1.0.2.20060820205737.022fe018-at-osu.edu} 13, 26 -- MIME-version: 1.0 13, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 13, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 13, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 13, 26 -- References: {200608201455.k7KEtawS007511-at-ns.microscopy.com} ==============================End of - Headers==============================
As you may know we run maintenance courses for all types of SEM and TEM and whilst I agree with most of what has been said may I add a point that may help those who are into the first few years of an instrument?
Today you may have PCs, memory, mice, keyboards, printers that are the same as your "new" microscope. In a few years or even weeks the PC you have will become obsolete - DO NOT THROW IT AWAY! In a few years time you may need parts for your microscope that will no longer be available - PCs, memory, mice, keyboards, printers - see what I mean?
Good luck enjoy your new machine, but think ahead!
Steve Chapman Senior Consultant Protrain For electron microscopy consultancy and training world wide Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967 Web www.emcourses.com
----- Original Message ----- X-from: {gary-at-gaugler.com} To: {protrain-at-emcourses.com} Sent: Sunday, August 20, 2006 1:37 AM
==============================Original Headers============================== 9, 33 -- From protrain-at-emcourses.com Mon Aug 21 07:24:34 2006 9, 33 -- Received: from rd01.mail.x-isp.net (rd01.mail.x-isp.net [213.253.171.197]) 9, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7LCOUlb027469 9, 33 -- for {microscopy-at-microscopy.com} ; Mon, 21 Aug 2006 07:24:33 -0500 9, 33 -- Received: from [62.69.65.166] (helo=smtp-c.mail.legend.co.uk) 9, 33 -- by rd01.mail.x-isp.net with smtp (Exim 4.60) 9, 33 -- (envelope-from {protrain-at-emcourses.com} ) 9, 33 -- id 1GF8pS-0005nc-0D 9, 33 -- for microscopy-at-microscopy.com; Mon, 21 Aug 2006 13:24:30 +0100 9, 33 -- Received: (qmail 24839 invoked from network); 21 Aug 2006 12:24:23 -0000 9, 33 -- Received: from unknown (HELO advent) (212.248.148.142) 9, 33 -- by 0 with SMTP; 21 Aug 2006 12:24:23 -0000 9, 33 -- Message-ID: {00db01c6c51c$f5f66680$0300a8c0-at-advent} 9, 33 -- Reply-To: "Steve Chapman" {protrain-at-emcourses.com} 9, 33 -- From: "Steve Chapman" {protrain-at-emcourses.com} 9, 33 -- To: {gary-at-gaugler.com} 9, 33 -- Cc: "American Soc" {microscopy-at-microscopy.com} 9, 33 -- References: {200608200037.k7K0bo8e026515-at-ns.microscopy.com} 9, 33 -- Subject: Re: [Microscopy] Re: New subject-service life of new SEMs 9, 33 -- Date: Mon, 21 Aug 2006 12:05:08 +0100 9, 33 -- Organization: Protrain 9, 33 -- MIME-Version: 1.0 9, 33 -- Content-Type: text/plain; 9, 33 -- format=flowed; 9, 33 -- charset="iso-8859-1"; 9, 33 -- reply-type=original 9, 33 -- Content-Transfer-Encoding: 7bit 9, 33 -- X-Priority: 3 9, 33 -- X-MSMail-Priority: Normal 9, 33 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2527 9, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2527 9, 33 -- X-tmReceived: from [62.69.65.166] (helo=smtp-c.mail.legend.co.uk) 9, 33 -- by rd01.mail.x-isp.net with smtp id 1GF8pS-0005nc-0D;Mon, 21 Aug 2006 13:24:30 +0100 ==============================End of - Headers==============================
Something sound wrong. We have a D50, a D200, and an FE these all use the standard nikon F-mount, and we have routinely switched between these without any problems. The D70 also has an F-mount, I have not tried it specifically but it should be a straight mount. You should NOT need a T-mount adapter (unless you actually have F-mount to T-mount adapters on your FE2 and FTN already), and your microscope phototube mount is a T-mount.
Now, what you will find is that the D70 (like the D50, D80, and D100), will only work in manual exposure mode, as it will treat the microscope mount as a Non-CPU lens.
Now for the D50 and D200, what we have are f-mounts on our phototubes (off of Nikon and Olympus scopes), 2X or 2.5X photo- eye pieces work well, and Nikon right-angle viewfinders. We use the cameras in tethered mode: AC-adapters, USB connections to computers, and run the Nikon Capture 4 software. The nikon software runs the camera directly from the computer (exposure, download, and shutter release). The software is not cheap (Capture 4 is $99, Capture NX is $149). The alternative is an electronic cable release, about $25, (You do NOT need the $150 Nikon remote release) but you still need to down load the images from the memory card.
Now a warning: The D50 has signifcant vibration due to the mirror, and like the D70, it does not have a mirror lock-up feature. When using the D50 on our Nikon Optiphot (very similar to the Diaphot) the vibrations are such that the images with 60X and 100X objectives are not useable, 40X is poor but useable. I do not know about the D70's vibration. We just got through testing the D200, and it inherently has much much lower vibration than the D50, and secondly it does have a "Mirror up, shutter delay".
Good luck.
On 18 Aug 2006, at 18:06, KLRadke-at-ucdavis.edu wrote:
} } Email: KLRadke-at-ucdavis.edu } Name: Kathryn Radke } } Organization: Univ. California, Davis } } Title-Subject: [Filtered] LM - coupling digital camera back to Nikon } Diaphot } } Question: I'd like to use my Nikon D70s camera back on my lab's 1985 } Nikon Diaphot inverted cell culture microscope. The Diaphot front port } has a fitting that couples to Nikon film backs (e.g. FE2, FTN) having } Nikon F bayonet type lens mount. When the microscope is set up } properly, the image viewed through the eyepieces focused on the film. } } The D70S back won't fit directly onto the Diaphot. A local camera } store tells me that I could use a T mount adapter that is designed to } couple a digital back with a telescope. With the T mount adapter in } place, the digital back can be used in Manual mode and the shutter } speed can be set using the "command dial" on the camera back. I would } also need to purchase a remote cord to operate the back without } shaking the microscope. } } I would be most grateful if anyone can give some feedback about } necessary parts, operation, etc. Are there websites that have } relevant information? } } Thank you. } Kathryn } } } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== 9, 12 -- From } zaluzec-at-microscopy.com Fri Aug 18 18:05:52 2006 9, 12 -- Received: } from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 9, 12 -- by } ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } k7IN5pPG017456 9, 12 -- for {microscopy-at-microscopy.com} ; Fri, 18 Aug } 2006 18:05:52 -0500 9, 12 -- Mime-Version: 1.0 9, 12 -- X-Sender: } (Unverified) 9, 12 -- Message-Id: } {p06110401c10bf8404212-at-[206.69.208.22]} 9, 12 -- Date: Fri, 18 Aug } 2006 18:05:50 -0500 9, 12 -- To: microscopy-at-microscopy.com 9, 12 -- } From: KLRadke-at-ucdavis.edu (by way of MicroscopyListserver) 9, 12 -- } Subject: viaWWW: LM - coupling digital camera back to Nikon Diaphot 9, } 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers==============================
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 13, 25 -- From edelmare-at-muohio.edu Mon Aug 21 08:14:58 2006 13, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7LDEwxr006149 13, 25 -- for {microscopy-at-Microscopy.com} ; Mon, 21 Aug 2006 08:14:58 -0500 13, 25 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 13, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id k7LDEuwv002611; 13, 25 -- Mon, 21 Aug 2006 09:14:56 -0400 13, 25 -- Received: from emf03 ([134.53.14.97]) 13, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id k7LDEto0008096; 13, 25 -- Mon, 21 Aug 2006 09:14:56 -0400 13, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 13, 25 -- To: KLRadke-at-ucdavis.edu 13, 25 -- Date: Mon, 21 Aug 2006 09:17:00 -0400 13, 25 -- MIME-Version: 1.0 13, 25 -- Content-type: text/plain; charset=US-ASCII 13, 25 -- Content-transfer-encoding: 7BIT 13, 25 -- Subject: Re: [Microscopy] viaWWW: LM - coupling digital camera back to Nikon Diaphot 13, 25 -- CC: microscopy-at-Microscopy.com 13, 25 -- Message-ID: {44E97A0C.28389.DCD8C08-at-localhost} 13, 25 -- Priority: normal 13, 25 -- In-reply-to: {200608182306.k7IN6bYr020112-at-ns.microscopy.com} 13, 25 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 13, 25 -- X-Real-ConnectIP: 134.53.14.97 13, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 13, 25 -- X-Scanned-By: MIMEDefang 2.52 on 134.53.6.11 ==============================End of - Headers==============================
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Email: aleb-at-nhm.ac.uk Name: Alex Ball
Organization: the Natural history Museum, London
Title-Subject: [Filtered] US - based EM suppliers
Question: Dear all,
With the current restrictions on flying with liquids/gels I am trying to get a list of US and European suppliers of EM chemicals, specifically fixatives.
I would be very grateful if you could forward to me details of suppliers who you think might be willing to send out EM grade glutaraldehyde in 10ml aliquots (glass ampoules).
I am aware of EMS and Ted Pella, although I do not have either of their US catalogues (I'll be writing to them formally in the near future).
Also, one of my colleagues spent some time working in a marine lab and was able to source pre-buffered glutaraldehyde, ready mixed to 2.5% concentration and 0.1M buffered to 7.2pH. Of course he forgot to take details of the supplier!!!! Has anyone seen this? I'd love to track this down as it would make supplying fixatives for our field researchers so easy.
Regards and thanks in advance,
Alex Ball E M unit manager, The Natural History Museum, London (Formerly B.M.(N.H.))
The coupling problem is probably caused by a ring around the camera mount attached to the microscope or camera adapter unit. The ring does not interfere with mounting the film cameras designed to mount on the microscope, but prevents mounting of some digital SLR F-mount cameras. The ring, which seems to be entirely cosmetic, is held in place with fine screws and is easily removed. Once the ring is removed the digital camera should lock in place properly.
The problem of camera vibration can be solved by setting the manual time exposure to some large value like 4 seconds and using one of the exposure control units made by Nikon (AFX, UFX etc) to control the exposure. Trigger the camera, wait a second, then trigger the exposure control unit which opens and closes the shutter in the camera adapter unit that was designed for 35mm film cameras. If you don't have one of these adapters and control units, they regularly show up on eBay.
The sensor unit of digital cameras is smaller than 35mm film format (except for the Kodak DCS 14 megapixel cameras) so you should use the 2.5x photo eyepiece rather than the 4x or 5x.
Ralph Common Division of Human Pathology Michigan State University
==============================Original Headers============================== 6, 24 -- From rcommon-at-msu.edu Mon Aug 21 11:07:23 2006 6, 24 -- Received: from sys21.mail.msu.edu (sys21.mail.msu.edu [35.9.75.121]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7LG7NuX029919 6, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Aug 2006 11:07:23 -0500 6, 24 -- Received: from [35.9.122.125] (helo=emlab) 6, 24 -- by sys21.mail.msu.edu with esmtpsa (Exim 4.52 #1) 6, 24 -- (TLSv1:RC4-MD5:128) 6, 24 -- id 1GFCJ9-00042W-CR 6, 24 -- for Microscopy-at-microscopy.com; Mon, 21 Aug 2006 12:07:23 -0400 6, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 6, 24 -- To: {Microscopy-at-microscopy.com} 6, 24 -- Subject: LM - coupling digital camera back to Nikon Diaphot 6, 24 -- Date: Mon, 21 Aug 2006 12:08:33 -0400 6, 24 -- Message-ID: {000001c6c53c$0e145140$7d7a0923-at-msu.edu} 6, 24 -- MIME-Version: 1.0 6, 24 -- Content-Type: text/plain; 6, 24 -- charset="iso-8859-1" 6, 24 -- Content-Transfer-Encoding: 7bit 6, 24 -- X-Priority: 3 (Normal) 6, 24 -- X-MSMail-Priority: Normal 6, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 6, 24 -- Importance: Normal 6, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1807 6, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
The general construction and functioning of cold cathode discharge gauges is discussed and illustrated in Sect. 3.2.2 (p. 99) of my book Vacuum Methods in Electron Microscopy (available from SPI Supplies, Ladd, M. E. Taylor, etc.). In particular, the photo of a disassembled gauge tube shown on page 101 might be helpful in giving you an idea of what to expect when taking your gauge tube apart for cleaning. The cleaning of Penning gauges is a rather common operation, so don't be afraid to undertake it - just be careful, and proceed with caution. In particular, don't get steel wool or any other magnetic cleaning agent in the neighborhood of the magnets that are part of the gauge, or you'll have a VERY difficult time getting the residue of the off.
The cleaning procedure recommended by Valery sounds like a simple one, so give it a try. You might also want to look over the comments about cleaning procedures in general given on page 70 of my book.
Good luck, WCB -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-engin.umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 3, 14 -- From bigelow-at-engin.umich.edu Mon Aug 21 15:02:45 2006 3, 14 -- Received: from srvr22.engin.umich.edu (srvr22.engin.umich.edu [141.213.75.21]) 3, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7LK2jxg011940 3, 14 -- for {microscopy-at-microscopy.com} ; Mon, 21 Aug 2006 15:02:45 -0500 3, 14 -- Received: from [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 3, 14 -- by srvr22.engin.umich.edu (8.13.6/8.13.6) with ESMTP id k7LK2iYV015998 3, 14 -- for {microscopy-at-microscopy.com} ; Mon, 21 Aug 2006 16:02:45 -0400 (EDT) 3, 14 -- Mime-Version: 1.0 3, 14 -- Message-Id: {p06210202c10fbcd1bac3-at-[141.212.131.221]} 3, 14 -- Date: Mon, 21 Aug 2006 16:02:43 -0400 3, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 14 -- From: Wil Bigelow {bigelow-at-engin.umich.edu} 3, 14 -- Subject: [Microscopy] RE: Cleaning Penning gauges 3, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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Email: a.ball-at-nhm.ac.uk Name: Alex Ball
Organization: Natural history Museum, London
Title-Subject: [Filtered] US - based EM suppliers - revised question
Question: Dear all,
Earlier today i posted a question about EM fixatives and i have had several useful replies. However, I don't think I was clear about why I wanted details of US suppliers.
Many of the researchers from the Natural History Museum conduct fieldwork overseas where they collect and fix specimens in the field or within other laboratories. In the past we supplied fixatives and buffers for them to take with them. The regulations over transport of small amounts of fixatives were sufficiently fuzzy for this type of material to be carried aboard aircraft without question.
Our post-911 world means this is no longer practical, so I was hoping to gather a list of useful contacts or suppliers so that researchers flying into the continental USA, including South America might be able to arrange for delivery of fixatives to them in order to still be able to fix material in the field.
Several people have sent me details of UK-based suppliers, but it's the the US-based ones that I really need to make contact with.
Sorry for the confusion and thanks for the leads sent to me so far,
Regards,
Alex
Dr Alex Ball Electron Microscope Unit Manager The Natural History Museum London SW7 9BD
Greetings, Can anyone tell me a reliable method to stain starch grains in sections of plant tissue embedded in Spurr's resin? Also useful to know whether polarized light would be suitable. I hope this is easy and I get lots of replies!
As ever, Tobias -- _ ____ __ ____ / \ / / \ / \ \ Tobias I. Baskin / / / / \ \ \ Biology Department /_ / __ /__ \ \ \__ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / / \ \ \ Amherst, MA, 01003 / / ___ / \ \__/ \ ____ http://www.bio.umass.edu/biology/baskin/ Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
==============================Original Headers============================== 2, 16 -- From baskin-at-bio.umass.edu Tue Aug 22 09:20:42 2006 2, 16 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 2, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MEKgaO016331 2, 16 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 09:20:42 -0500 2, 16 -- Received: from [172.30.55.79] (eutopia [128.119.55.30]) 2, 16 -- by marlin.bio.umass.edu (8.13.6/8.13.6) with ESMTP id k7MEKcMV012516 2, 16 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 10:20:39 -0400 (EDT) 2, 16 -- Mime-Version: 1.0 2, 16 -- Message-Id: {p06230906c110c25e16f3-at-[172.30.55.79]} 2, 16 -- Date: Tue, 22 Aug 2006 10:20:38 -0400 2, 16 -- To: microscopy-at-microscopy.com 2, 16 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 2, 16 -- Subject: staining starch in sections 2, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 2, 16 -- X-Greylist: Sender succeded SMTP AUTH authentication, not delayed by milter-greylist-1.6 (marlin.bio.umass.edu [128.119.55.19]); Tue, 22 Aug 2006 10:20:39 -0400 (EDT) 2, 16 -- X-Scanned-By: MIMEDefang 2.54 on 128.119.55.19 ==============================End of - Headers==============================
I think I have an easy solution for a common problem and I just wanted to share it. I am sorry if I bring nothing new, but perhaps it really helps. At least it cannot harm :-)
When working with my TEMcam Megaview III and the software AnalySIS (no personal interest blablabla...) the pictures are saved in 16bit TIFF format. This is a problem when you want to work with them because this format is not recognized by most image processing software (This problem is so common that it appears in the FAQ of AnalySIS). However when this format is loaded in Adobe Photoshop (no personal....) the picture comes completely dark. A rather simple solution to recover your picture is to use the "auto contrast" and/or "auto level" option and your nice picture appears in all its splendor right before your eyes!
Hope this was helpful. Have a nice day (for the american), a nice sleep (for the australian) or a nice evening (for the european).
Stephane
__________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com
==============================Original Headers============================== 7, 18 -- From nizets2-at-yahoo.com Tue Aug 22 10:22:55 2006 7, 18 -- Received: from web37410.mail.mud.yahoo.com (web37410.mail.mud.yahoo.com [209.191.91.142]) 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k7MFMtJ5027602 7, 18 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 10:22:55 -0500 7, 18 -- Received: (qmail 21837 invoked by uid 60001); 22 Aug 2006 15:22:55 -0000 7, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 18 -- s=s1024; d=yahoo.com; 7, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 7, 18 -- b=FP2WVswviXCCARHnXwNLh4HVu9wy6B+uLcz1srxmBXfOK7AOK1yXsSzEW/ImrlWN4trgP7WdCahgLGDn8k2mb/DEqR/6OtgyruYcPY4CSc5LB0WGkWkbw38+DvR5OuvAvRI51DYVL4elyoXJJaPVjoBn3cOnsVMP+LHoCAm6+yA= ; 7, 18 -- Message-ID: {20060822152255.21835.qmail-at-web37410.mail.mud.yahoo.com} 7, 18 -- Received: from [80.122.101.102] by web37410.mail.mud.yahoo.com via HTTP; Tue, 22 Aug 2006 08:22:55 PDT 7, 18 -- Date: Tue, 22 Aug 2006 08:22:55 -0700 (PDT) 7, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 18 -- Subject: 16bit TIFF images in Photoshop 7, 18 -- To: microscopy-at-microscopy.com 7, 18 -- MIME-Version: 1.0 7, 18 -- Content-Type: text/plain; charset=iso-8859-1 7, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Okay, 99% of all my ultra-microtomy has been done with epoxy/resin embedded 'biological' samples.
Recently I was asked about cutting a thin bi-metalic film on the surface of a silicon wafer.
My initial gut reaction to this was "cut a Si wafer in cross-section with one of our diamond knives? Are you kidding?"
For those out there more immersed in the materials side of TEM prep, can a Si wafer be cut in cross section with a regular 45º Ultra Diamond knife? Would thinning the wafer down prior to deposition make it easier to cut? Or easier on the knife? If possible to cut, would these sections just be floated off in water like standard samples? Or best to leave dry and manipulate with eye-lashes or Dalmatian hair.
Thank you for bearing with these questions on materials prep from a biological based pov. And no, this is the only way to get this specific film thin enough (cutting), Ion Mill, polishing, no other 'typical' thinning prep works.
Geoff Williams Leduc Bioimaging Facility Manager Brown University
16 bit TIFFs are a fairly common format for scientific image data, and there are a variety of programs intended for scientific use which support them. In addition to many commercial products both cheaper and more expensive than Photoshop (DigitalMicrograph, IDL, Igor Pro, etc. etc.) there is an excellent, free program called ImageJ available from
{http://rsb.info.nih.gov/ij/}
It reads and displays the 16 bit TIFF images from our MegaVIEW / AnalySIS system with no additional steps.
There is also a potentially serious drawback to the Photoshop procedure you suggest. Photoshop discards the original grey-scale values of the image when you use "Auto Levels", which breaks the quantitative connection between the grey-scale value in the image and the absolute number of electrons that struck the detector. This will often make quantitative analysis of the electron image or diffraction pattern impossible and makes it difficult to even judge the relative average brightness of two different images to guess, for example, the sample thickness.
I apologize for the long reply, but this is one of my pet peeves: what comes off the microscope has the potential to be quantitative data, not just pictures. Software developed for photographs and desktop publishing is not generally the best choice for dealing with scientific data.
Best wishes, Paul Voyles
Paul Voyles Materials Science and Engineering University of Wisconsin, Madison 1509 University Ave, Rm 223 Madison, WI 53706-1595 voice: (608) 265-6740 fax: (608) 262-8353 voyles-at-engr.wisc.edu http://tem.msae.wisc.edu
nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear listers, } } I think I have an easy solution for a common problem } and I just wanted to share it. I am sorry if I bring } nothing new, but perhaps it really helps. At least it } cannot harm :-) } } When working with my TEMcam Megaview III and the } software AnalySIS (no personal interest blablabla...) } the pictures are saved in 16bit TIFF format. This is a } problem when you want to work with them because this } format is not recognized by most image processing } software (This problem is so common that it appears in } the FAQ of AnalySIS). However when this format is } loaded in Adobe Photoshop (no personal....) the } picture comes completely dark. A rather simple } solution to recover your picture is to use the "auto } contrast" and/or "auto level" option and your nice } picture appears in all its splendor right before your } eyes! } } Hope this was helpful. } Have a nice day (for the american), a nice sleep (for } the australian) or a nice evening (for the european). } } Stephane } } } __________________________________________________ } Do You Yahoo!? } Tired of spam? Yahoo! Mail has the best spam protection around } http://mail.yahoo.com
==============================Original Headers============================== 11, 25 -- From voyles-at-engr.wisc.edu Tue Aug 22 10:45:08 2006 11, 25 -- Received: from mail.cae.wisc.edu (mail.cae.wisc.edu [144.92.13.31]) 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MFj8iP016024 11, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 10:45:08 -0500 11, 25 -- Received: from portkey.cae.wisc.edu (portkey.cae.wisc.edu [144.92.13.118]) 11, 25 -- by mail.cae.wisc.edu (8.13.3+Sun/8.13.4) with ESMTP id k7MFiwKs006707 11, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 10:44:58 -0500 (CDT) 11, 25 -- Received: from [128.104.200.57] (voylespad1.msae.wisc.edu [128.104.200.57]) 11, 25 -- (authenticated bits=0) 11, 25 -- by portkey.cae.wisc.edu (8.13.4/8.13.4/Debian-3sarge1) with ESMTP id k7MFiwoJ032645 11, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 11, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 10:44:58 -0500 11, 25 -- Message-ID: {44EB2674.5020705-at-engr.wisc.edu} 11, 25 -- Date: Tue, 22 Aug 2006 10:44:52 -0500 11, 25 -- From: Paul Voyles {voyles-at-engr.wisc.edu} 11, 25 -- User-Agent: Thunderbird 1.5.0.5 (Windows/20060719) 11, 25 -- MIME-Version: 1.0 11, 25 -- To: Microscopy-at-microscopy.com 11, 25 -- Subject: Re: [Microscopy] 16bit TIFF images in Photoshop 11, 25 -- References: {200608221524.k7MFO45e029784-at-ns.microscopy.com} 11, 25 -- In-Reply-To: {200608221524.k7MFO45e029784-at-ns.microscopy.com} 11, 25 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 11, 25 -- Content-Transfer-Encoding: 7bit 11, 25 -- X-CAE-MailScanner-Information: Please contact security-at-engr.wisc.edu if this message contains a virus or has been corrupted in delivery. 11, 25 -- X-CAE-MailScanner: Found to be clean (benji) ==============================End of - Headers==============================
I do not know exactly what I can divulge, but they have tried tripod polishing, and ion-milling but I am told they elevate the temperature and the layers need to be prepped and kept at room temps until imaging.
Does that say enough? I still feel it's a bit irrelevant to the initial question.
Is it possible to section a Si wafer? Am I correct in being skeptical? Or should I just kill a $4k knife or try an ancient already messed up and questionably anchored diamond in the attempt?
I just figured someone out there might be able to share a bit of practical experience.
Thanks!
Geoff Williams Leduc Bioimaging Facility Manager Brown University
Try the freeware Autostitch ( http://www.autostitch.net ). It is free, it works very well even if the interface of the demo version is crude and basic.
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Jennifer,
I have used Panavue ImageAssembler (http://www.panavue.com/) with some success. You do have to load the images in row order. I've sometimes had to stitch the rows first then assemble the rows into the final image rather than assembling the whole image in one pass. ImageAssembler can use either automatic or manual registration for the stitching. The auto registration works reasonably well for image sets with reasonable overlap (20-25%) in the images and if the features are easily distinguishable and not too complex. (I suspect that it just does a cross-correlation.) It will do some image warping and brightness/contrast adjustment to better blend the photos in the stitching process.
The software is pretty easy to use and the price is reasonable ($64 for the standard edition and $129 for the professional edition). The professional version will handle larger images.
The software has worked for me. Your mileage may vary!
Usual disclaimer: I have no vested interest in the product or company.
Good Luck, Henk Colijn
At 10:55 AM 8/20/2006, jds451-at-psu.edu wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility www.ceof.ohio-state.edu 040 Fontana Labs, 116 W. 19th Ave
==============================Original Headers============================== 13, 26 -- From colijn.1-at-osu.edu Sun Aug 20 20:19:13 2006 13, 26 -- Received: from ER6S1.ENG.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7L1JClO007521 13, 26 -- for {microscopy-at-microscopy.com} ; Sun, 20 Aug 2006 20:19:13 -0500 13, 26 -- Received: from CONVERSION-DAEMON.er6s1.eng.ohio-state.edu by 13, 26 -- er6s1.eng.ohio-state.edu (PMDF V6.2-1x11 #31056) 13, 26 -- id {01M689NUXPKGA8NPYQ-at-er6s1.eng.ohio-state.edu} for 13, 26 -- microscopy-at-microscopy.com; Sun, 20 Aug 2006 21:19:10 -0400 (EDT) 13, 26 -- Received: from HOC3.osu.edu 13, 26 -- (d149-67-33-174.col.wideopenwest.com [67.149.174.33]) 13, 26 -- by er6s1.eng.ohio-state.edu (PMDF V6.2-1x11 #31056) 13, 26 -- with ESMTPA id {01M689NU56XYA8MMTU-at-er6s1.eng.ohio-state.edu} ; Sun, 13, 26 -- 20 Aug 2006 21:19:10 -0400 (EDT) 13, 26 -- Date: Sun, 20 Aug 2006 21:18:00 -0400 13, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 13, 26 -- Subject: Re: [Microscopy] Software: Montage 13, 26 -- In-reply-to: {200608201455.k7KEtawS007511-at-ns.microscopy.com} 13, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 13, 26 -- To: jds451-at-psu.edu 13, 26 -- Cc: microscopy-at-microscopy.com 13, 26 -- Message-id: {7.0.1.0.2.20060820205737.022fe018-at-osu.edu} 13, 26 -- MIME-version: 1.0 13, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 13, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 13, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 13, 26 -- References: {200608201455.k7KEtawS007511-at-ns.microscopy.com} ==============================End of - Headers==============================
==============================Original Headers============================== 24, 26 -- From jean-paul.bailon-at-polymtl.ca Tue Aug 22 11:04:57 2006 24, 26 -- Received: from smtp.polymtl.ca (smtp.polymtl.ca [132.207.4.11]) 24, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MG4vVr002519 24, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 11:04:57 -0500 24, 26 -- Received: from jpbailon (jmdorlot.metal.polymtl.ca [132.207.53.29]) 24, 26 -- by smtp.polymtl.ca (8.13.6/8.13.6) with ESMTP id k7MG4qdA017147 24, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 12:04:56 -0400 24, 26 -- Return-Receipt-To: "Jean-Paul Bailon" {jean-paul.bailon-at-polymtl.ca} 24, 26 -- Reply-To: {jean-paul.bailon-at-polymtl.ca} 24, 26 -- From: "Jean-Paul Bailon" {jean-paul.bailon-at-polymtl.ca} 24, 26 -- To: {Microscopy-at-microscopy.com} 24, 26 -- Subject: RE: [Microscopy] Re: Software: Montage 24, 26 -- Date: Tue, 22 Aug 2006 12:05:05 -0400 24, 26 -- Organization: Ecole Polytechnique de Montreal 24, 26 -- Message-ID: {!&!AAAAAAAAAAAYAAAAAAAAAIv5ENKzUdMRv4cAUAR8QvvCgAAAEAAAAPPNakJfCY9HnJXfsHX2hyABAAAAAA==-at-polymtl.ca} 24, 26 -- MIME-Version: 1.0 24, 26 -- Content-Type: text/plain; 24, 26 -- charset="iso-8859-1" 24, 26 -- X-Mailer: Microsoft Office Outlook 11 24, 26 -- In-Reply-To: {200608210120.k7L1KdsH009154-at-ns.microscopy.com} 24, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 24, 26 -- Thread-Index: AcbEwAaXEu6ez8u1S7G5U6mzqzR6eABQ9fJw 24, 26 -- Disposition-Notification-To: "Jean-Paul Bailon" {jean-paul.bailon-at-polymtl.ca} 24, 26 -- X-Poly-FromMTA: (jmdorlot.metal.polymtl.ca [132.207.53.29]) at Tue, 22 Aug 2006 16:04:52 +0000 24, 26 -- Content-Transfer-Encoding: 8bit 24, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k7MG4vVr002519 ==============================End of - Headers==============================
Hi Geoffrey, A similar question came up a while ago about samples getting too hot during prep or in the TEM itself. I've managed to prepare Au:Ge and Pd:Zn:Au metal layers on III-Vs using fairly conventional techniques; these metals interdiffuse below 100C, so unless you have to keep the temperature really low for your samples it should be possible. The only difference to my standard protocol is to use superglue to mount the sample rather than thermoplastic wax, and soak the sample off in acetone (which I think is the standard practice for tripod polishing anyway?). Since I work with III-Vs like InP I always have to keep the energy going into the sample to a minimum during ion miling, but I find I can hit it with 6 kV Ar+ ions in a PIPS as long as I keep the incidence angle below 3 degrees. However when I was using an ion tech mill which didn't have a modulated beam or go below 10 degrees incidence I had to use LN2 cooling. Contact me off list if you want more details. As for microtomy, I have no idea. I have heard of it being done and read a paper or two, but I haven't got any further than being envious of the idea of making an electron transparent TEM sample in less that 10 mins.. or perhaps that's just my fantasy of how quick microtomy can be..
-----Original Message----- X-from: Geoffrey_Williams-at-brown.edu [mailto:Geoffrey_Williams-at-brown.edu] Sent: 22 August 2006 16:33 To: Richard Beanland
Okay, 99% of all my ultra-microtomy has been done with epoxy/resin embedded 'biological' samples.
Recently I was asked about cutting a thin bi-metalic film on the surface of a silicon wafer.
My initial gut reaction to this was "cut a Si wafer in cross-section with one of our diamond knives? Are you kidding?"
For those out there more immersed in the materials side of TEM prep, can a Si wafer be cut in cross section with a regular 45º Ultra Diamond knife? Would thinning the wafer down prior to deposition make it easier to cut? Or easier on the knife? If possible to cut, would these sections just be floated off in water like standard samples? Or best to leave dry and manipulate with eye-lashes or Dalmatian hair.
Thank you for bearing with these questions on materials prep from a biological based pov. And no, this is the only way to get this specific film thin enough (cutting), Ion Mill, polishing, no other 'typical' thinning prep works.
Geoff Williams Leduc Bioimaging Facility Manager Brown University
==============================Original Headers============================== 8, 29 -- From Geoffrey_Williams-at-brown.edu Tue Aug 22 10:32:17 2006 8, 29 -- Received: from draco.services.brown.edu (draco.services.brown.edu [128.148.106.172]) 8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MFWG1H005521 8, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 10:32:17 -0500 8, 29 -- Received: from ex-gateway2-out.AD.Brown.Edu (ex-gateway2.ad.brown.edu [128.148.21.53]) 8, 29 -- by draco.services.brown.edu (Switch-3.1.8/Switch-3.1.7/) with ESMTP id k7MFWGMw023143 8, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 11:32:16 -0400 (EDT) 8, 29 -- Received: from mail1.AD.Brown.Edu ([128.148.21.30]) by ex-gateway2-out.AD.Brown.Edu with Microsoft SMTPSVC(6.0.3790.1830); 8, 29 -- Tue, 22 Aug 2006 11:32:16 -0400 8, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 8, 29 -- Content-class: urn:content-classes:message 8, 29 -- MIME-Version: 1.0 8, 29 -- Content-Type: text/plain; 8, 29 -- charset="iso-8859-1" 8, 29 -- Subject: Sectioning Si Wafers 8, 29 -- Date: Tue, 22 Aug 2006 11:32:15 -0400 8, 29 -- Message-ID: {A1A84D541C161C4C988B4E0FB38F5A0F047F6786-at-MAIL1.AD.Brown.Edu} 8, 29 -- X-MS-Has-Attach: 8, 29 -- X-MS-TNEF-Correlator: 8, 29 -- Thread-Topic: Sectioning Si Wafers 8, 29 -- Thread-Index: AcbGACVCTVY7vLTfSbKUtWaWeb/iew== 8, 29 -- From: "Williams, Geoffrey" {Geoffrey_Williams-at-brown.edu} 8, 29 -- To: {Microscopy-at-microscopy.com} 8, 29 -- X-OriginalArrivalTime: 22 Aug 2006 15:32:16.0556 (UTC) FILETIME=[25F216C0:01C6C600] 8, 29 -- X-Brown-Proofpoint: Not Infected 8, 29 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 mlx=-1 adultscore=0 adjust=0 reason=safe engine=3.1.0-0608080000 definitions=main-0608220002 8, 29 -- X-Brown-MailScanner-SpamCheck: not spam, rule=notspam policy=default score=0 mlx=-1 adultscore=0 adjust=0 reason=safe engine=3.1.0-0608080000 definitions=main-0608220002 8, 29 -- Content-Transfer-Encoding: 8bit 8, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k7MFWG1H005521 ==============================End of - Headers==============================
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==============================Original Headers============================== 21, 32 -- From richard.beanland-at-bookham.com Tue Aug 22 11:24:29 2006 21, 32 -- Received: from mail80.messagelabs.com (mail80.messagelabs.com [195.245.230.163]) 21, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k7MGOSqH014905 21, 32 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 11:24:29 -0500 21, 32 -- X-VirusChecked: Checked 21, 32 -- X-Env-Sender: richard.beanland-at-bookham.com 21, 32 -- X-Msg-Ref: server-4.tower-80.messagelabs.com!1156263867!1189339!1 21, 32 -- X-StarScan-Version: 5.5.10.7; banners=bookham.com,-,- 21, 32 -- X-Originating-IP: [213.249.209.179] 21, 32 -- Received: (qmail 7650 invoked from network); 22 Aug 2006 16:24:27 -0000 21, 32 -- Received: from unknown (HELO cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 21, 32 -- by server-4.tower-80.messagelabs.com with SMTP; 22 Aug 2006 16:24:27 -0000 21, 32 -- Received: from cas-smx-02.BOOKHAM.ENTERPRISE.PRI ([10.4.0.223]) by cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI with Microsoft SMTPSVC(6.0.3790.1830); 21, 32 -- Tue, 22 Aug 2006 17:26:47 +0100 21, 32 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 21, 32 -- Content-class: urn:content-classes:message 21, 32 -- MIME-Version: 1.0 21, 32 -- Content-Type: text/plain; 21, 32 -- charset="iso-8859-1" 21, 32 -- Subject: RE: [Microscopy] Sectioning Si Wafers 21, 32 -- Date: Tue, 22 Aug 2006 17:26:49 +0100 21, 32 -- Message-ID: {9645D3E33E4C6548B12A7B25F611533E1BA33A-at-cas-smx-02.BOOKHAM.ENTERPRISE.PRI} 21, 32 -- X-MS-Has-Attach: 21, 32 -- X-MS-TNEF-Correlator: 21, 32 -- Thread-Topic: [Microscopy] Sectioning Si Wafers 21, 32 -- Thread-Index: AcbGAJfjm2P9z+WlQLCh7Ucg5/QC4QABIvnw 21, 32 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 21, 32 -- To: {Geoffrey_Williams-at-brown.edu} 21, 32 -- Cc: {microscopy-at-microscopy.com} 21, 32 -- X-OriginalArrivalTime: 22 Aug 2006 16:26:47.0846 (UTC) FILETIME=[C3C95460:01C6C607] 21, 32 -- Content-Transfer-Encoding: 8bit 21, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k7MGOSqH014905 ==============================End of - Headers==============================
Cryo ion-milling is one option, FIB liftout is another. A third option is the small-angle cleavage technique that John McCaffrey and Scott Walck developed. The cleavage technique requires some practice, but if done right the fracture should propagate right through the surface film and give you thin damage free samples without heating.
Scott is now at Southbay Technology (walck-at-southbaytech.com) but monitors the listserver. (Scott, Would you like to add anything?)
Cheers, Henk Colijn
At 12:01 PM 08/22/06, Geoffrey_Williams-at-brown.edu wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 11, 26 -- From colijn.1-at-osu.edu Tue Aug 22 12:50:09 2006 11, 26 -- Received: from ER6S1.ENG.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MHo91V027091 11, 26 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 12:50:09 -0500 11, 26 -- Received: from CONVERSION-DAEMON.er6s1.eng.ohio-state.edu by 11, 26 -- er6s1.eng.ohio-state.edu (PMDF V6.2-1x11 #31056) 11, 26 -- id {01M6AMKSUYR4A8MJ3P-at-er6s1.eng.ohio-state.edu} for 11, 26 -- microscopy-at-microscopy.com; Tue, 22 Aug 2006 13:50:08 -0400 (EDT) 11, 26 -- Received: from HOC1.ecr6.ohio-state.edu 11, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.eng.ohio-state.edu 11, 26 -- (PMDF V6.2-1x11 #31056) 11, 26 -- with ESMTPA id {01M6AMKRYQE0A8PT39-at-er6s1.eng.ohio-state.edu} ; Tue, 11, 26 -- 22 Aug 2006 13:50:07 -0400 (EDT) 11, 26 -- Date: Tue, 22 Aug 2006 13:51:34 -0400 11, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 11, 26 -- Subject: Re: [Microscopy] Sectioning Si, clarification 11, 26 -- In-reply-to: {200608221601.k7MG1qja029254-at-ns.microscopy.com} 11, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 11, 26 -- To: Geoffrey_Williams-at-brown.edu 11, 26 -- Cc: microscopy-at-microscopy.com, walck-at-southbaytech.com 11, 26 -- Message-id: {7.0.1.0.2.20060822134349.03791640-at-osu.edu} 11, 26 -- MIME-version: 1.0 11, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 11, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 11, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 11, 26 -- References: {200608221601.k7MG1qja029254-at-ns.microscopy.com} ==============================End of - Headers==============================
I was actually dumb enough to try microtoming thin films (non-metallic) on silicon wafers for awhile. I got the best results (this is a relative term) by first embedding small pieces of the wafer in epoxy (Embed 812), then using a polisher and diamond films to polish away most of the silicon from the back side. I think I wedged it slightly to obtain a red area of silicon . I then re-embedded the whole thing to encapsulate it in epoxy, then block trimmed to get to a nice thin area. You want to be cutting through the least amount of silicon possible. I used fairly slow cutting speeds and a water-filled boat. No problems picking up sections or placing on filmed grids, but the material beats up the knife fast so I kept having to move to another knife area.
As for the TEM results, we did get some useful pictures, but the silicon does not cut per se, it's more like a repeatable fracture. So you just look among the pieces for an intact place, which can take up some scope time just finding a good area. If you really cannot prepare your material with other methods (polishing/ion-milling, FIB), or you are trying to assess sample preparation artifacts from ion energies, then it can be useful. But it is tedious, both on the prep side and in the microscope.
I don't have experience with your type of material, but I would suggest first trying tripod polishing or dimpling, and low-energy/low-angle ion-milling.
Feel free to contact me offline if you would like more details.
Leslie
Leslie Krupp (Thompson) IBM Almaden Research 650 Harry Road, K19/D1 San Jose, CA 95120-6099 (408) 927-3856
==============================Original Headers============================== 7, 28 -- From lkrupp-at-us.ibm.com Tue Aug 22 12:59:55 2006 7, 28 -- Received: from e2.ny.us.ibm.com (e2.ny.us.ibm.com [32.97.182.142]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MHxtw5005044 7, 28 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 12:59:55 -0500 7, 28 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234]) 7, 28 -- by e2.ny.us.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id k7MHxoSn013452 7, 28 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 13:59:50 -0400 7, 28 -- Received: from d01av04.pok.ibm.com (d01av04.pok.ibm.com [9.56.224.64]) 7, 28 -- by d01relay02.pok.ibm.com (8.13.6/8.13.6/NCO v8.1.1) with ESMTP id k7MHxoX3294042 7, 28 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 13:59:50 -0400 7, 28 -- Received: from d01av04.pok.ibm.com (loopback [127.0.0.1]) 7, 28 -- by d01av04.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id k7MHxniq026488 7, 28 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 13:59:49 -0400 7, 28 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) 7, 28 -- by d01av04.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id k7MHxnur026485 7, 28 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 13:59:49 -0400 7, 28 -- In-Reply-To: {200608221631.k7MGVMQJ022467-at-ns.microscopy.com} 7, 28 -- To: microscopy-at-microscopy.com 7, 28 -- Subject: RE: Sectioning Si Wafers 7, 28 -- MIME-Version: 1.0 7, 28 -- X-Mailer: Lotus Notes Release 7.0 HF144 February 01, 2006 7, 28 -- Message-ID: {OFB5C3E18B.7BA6F5D9-ON852571D2.0062D1E7-882571D2.0062DCF0-at-us.ibm.com} 7, 28 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} 7, 28 -- Date: Tue, 22 Aug 2006 10:59:49 -0700 7, 28 -- X-MIMETrack: Serialize by Router on D01ML604/01/M/IBM(Release 7.0.1HF269 | June 22, 2006) at 7, 28 -- 08/22/2006 13:59:49, 7, 28 -- Serialize complete at 08/22/2006 13:59:49 7, 28 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
No you can't "cut" Si with a diamond knife. Si cleaves very easily. When the knife touches the Si, the Si cleaves along one of its preferred {111} crystallographic cleaving planes. That might be fine if you orient the Si such that a Si {111} crystallographic direction is perpendicular to the block face. Then if you are VERY lucky, you MIGHT get a cleaved piece of Si oriented with {111} faces (at the risk of your knife). Any article that I've seen that claims to have microtomed Si has shown lots of pictures of tiny {111} Si sections.
Assuming that you are successful, so what! There is no, or very little interest, in anything in the semiconductor world or in potentially oriented thin films deposited on Si, that is best displayed on top of a Si cross section with a {111} block face orientation. The angles are wrong.
Si wafers in semiconductor processing are almost all with (or very close to) a Si {001} direction perpendicular to the Si wafer surface. Devices fabricated in, or patterns deposited on, these wafers are not randomly oriented. They are almost always aligned with {011} planes perpendicular to the Si surface, i.e. the x, y orientations of the devices and patterns are parallel to {011} directions in the Si surface. Cross sections of interest for TEM or SEM analysis are oriented such that the microscope looks down on a {011} "block face." If you orient the specimen's block face so as to attempt to cut {011} cross sections, the diamond knife will touch the Si and immediately propagate a cleave down the nearest {111} plane despite the orientation of the block. As the specimen in the block is constrained by the epoxy holding it, the result is a flake of Si dust from just the surface. When the knife comes around again you get more dust.
Using a microtome to make Si {011}cross sections is like using a screw driver to drive a nail.
Instead of fighting the fact that Si cleaves, why not exploit this fact and prepare really great thin specimens by the microcleaving technique pioneered by McCaffrey and Igor. I'll copy them and invite them to expand on this suggestion. They'll point out that you can make nice thin sections of thin films on Si with a minimum of tools and at room temperature. There is a videotape that shows how to do it.
Ron Anderson
Geoffrey_Williams-at-brown.edu wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I do not know exactly what I can divulge, but they have tried tripod polishing, and ion-milling but I am told they elevate the temperature and the layers need to be prepped and kept at room temps until imaging. } } Does that say enough? I still feel it's a bit irrelevant to the initial question. } } Is it possible to section a Si wafer? Am I correct in being skeptical? Or should I just kill a $4k knife or try an ancient already messed up and questionably anchored diamond in the attempt? } } I just figured someone out there might be able to share a bit of practical experience. } } Thanks! } } Geoff Williams } Leduc Bioimaging Facility Manager } Brown University } } http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/ } } } } ==============================Original Headers============================== } 8, 29 -- From Geoffrey_Williams-at-brown.edu Tue Aug 22 10:59:55 2006 } 8, 29 -- Received: from draco.services.brown.edu (draco.services.brown.edu [128.148.106.172]) } 8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MFxtQK026422 } 8, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 10:59:55 -0500 } 8, 29 -- Received: from ex-gateway1-out.AD.Brown.Edu (ex-gateway1.ad.brown.edu [128.148.21.51]) } 8, 29 -- by draco.services.brown.edu (Switch-3.1.8/Switch-3.1.7/) with ESMTP id k7MFxs7W006189 } 8, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 11:59:54 -0400 (EDT) } 8, 29 -- Received: from mail1.AD.Brown.Edu ([128.148.21.30]) by ex-gateway1-out.AD.Brown.Edu with Microsoft SMTPSVC(6.0.3790.1830); } 8, 29 -- Tue, 22 Aug 2006 11:59:54 -0400 } 8, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 } 8, 29 -- Content-class: urn:content-classes:message } 8, 29 -- MIME-Version: 1.0 } 8, 29 -- Content-Type: text/plain; } 8, 29 -- charset="iso-8859-1" } 8, 29 -- Subject: Sectioning Si, clarification } 8, 29 -- Date: Tue, 22 Aug 2006 11:59:53 -0400 } 8, 29 -- Message-ID: {A1A84D541C161C4C988B4E0FB38F5A0F047F6793-at-MAIL1.AD.Brown.Edu} } 8, 29 -- X-MS-Has-Attach: } 8, 29 -- X-MS-TNEF-Correlator: } 8, 29 -- Thread-Topic: Sectioning Si, clarification } 8, 29 -- Thread-Index: AcbGBAF0V914xLqeQPmnA7STgTxe2Q== } 8, 29 -- From: "Williams, Geoffrey" {Geoffrey_Williams-at-brown.edu} } 8, 29 -- To: {Microscopy-at-microscopy.com} } 8, 29 -- X-OriginalArrivalTime: 22 Aug 2006 15:59:54.0245 (UTC) FILETIME=[02015B50:01C6C604] } 8, 29 -- X-Brown-Proofpoint: Not Infected } 8, 29 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 mlx=-1 adultscore=0 adjust=0 reason=safe engine=3.1.0-0608080000 definitions=main-0608220003 } 8, 29 -- X-Brown-MailScanner-SpamCheck: not spam, rule=notspam policy=default score=0 mlx=-1 adultscore=0 adjust=0 reason=safe engine=3.1.0-0608080000 definitions=main-0608220003 } 8, 29 -- Content-Transfer-Encoding: 8bit } 8, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k7MFxtQK026422 } ==============================End of - Headers============================== } } }
==============================Original Headers============================== 11, 21 -- From randerson20-at-tampabay.rr.com Tue Aug 22 13:16:47 2006 11, 21 -- Received: from ms-smtp-06.tampabay.rr.com (ms-smtp-06.tampabay.rr.com [65.32.5.136]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MIGj91015481 11, 21 -- for {Microscopy-at-Microscopy.Com} ; Tue, 22 Aug 2006 13:16:46 -0500 11, 21 -- Received: from [127.0.0.1] (rrcs-24-73-73-214.se.biz.rr.com [24.73.73.214]) 11, 21 -- by ms-smtp-06.tampabay.rr.com (8.13.6/8.13.6) with ESMTP id k7MIGVGD023421; 11, 21 -- Tue, 22 Aug 2006 14:16:37 -0400 (EDT) 11, 21 -- Message-ID: {44EB49FD.2020607-at-tampabay.rr.com} 11, 21 -- Date: Tue, 22 Aug 2006 14:16:29 -0400 11, 21 -- From: Ronald Anderson {randerson20-at-tampabay.rr.com} 11, 21 -- User-Agent: Thunderbird 1.5.0.5 (Windows/20060719) 11, 21 -- MIME-Version: 1.0 11, 21 -- To: Geoffrey_Williams-at-brown.edu, Listserver {Microscopy-at-Microscopy.Com} , 11, 21 -- John McCaffrey {mccaffrey2002-at-rogers.com} , 11, 21 -- Scott Walck {walck-at-southbaytech.com} 11, 21 -- Subject: Re: [Microscopy] Sectioning Si, clarification 11, 21 -- References: {200608221600.k7MG02nF026627-at-ns.microscopy.com} 11, 21 -- In-Reply-To: {200608221600.k7MG02nF026627-at-ns.microscopy.com} 11, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 21 -- Content-Transfer-Encoding: 7bit 11, 21 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
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Email: delsignore-at-gmail.com Name: Anthony Del Signore
Organization: Brown University
Education: Graduate College
Location: Providence, RI, 02865
Title: imaging of cellular aggregates
Question: I am trying to image an aggregate of cells that are producing GFP. I am running into problems when trying to image with Epifluorescent ant Confocal microscopes. I was wondering if I am using the right equipment. It seems that with the epifluor I get alot of deconvolved and out of focus light. With the confocal I am only able to image about 30 microns into the 200 micron spherical aggregate. I get a rim around the spheroid and then black within the aggregate. I am using a 10x objective. Are there any other methods for imaging... I have also heard of flow cytometry and two photon microscopy. Are these valid methods for imaging cellular aggregates emitting GFP? Any help, suggestions would be greatly appreciated. If you need additional information or clarification please let me know as well. Thanks and have a great day.
Metamorph from Molecular Devices works excellent but it is expensive.
You can try ImageJ (free), I have used for less than 50 images.
Zsolt
************************************** Zsolt Lazar Molecular Cytology Core Facility Memorial Sloan-Kettering Cancer Center 415 East 68th Street, ZRC-1838 New York, NY 10021
-----Eredeti üzenet----- Feladó: jds451-at-psu.edu [mailto:jds451-at-psu.edu] Küldve: V 8/20/06 11:04 CÃmzett: Lazar, Zsolt/Sloan-Kettering Institute Másolatot kap: Tárgy: [Microscopy] Software: Montage
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Hi all, I have a friend who needs to make a montage of a large number of optical micrographs. He has about 500 images. Can anybody suggest a PC software package that is easy to use? He is currently using Photoshop. Freeware or something that can be paid for and downloaded would be especially useful. Thanks for any suggestions,
Jennifer Ray Sloppy Materials Science & Engineering, Ph.D. Candidate Pennsylvania State University
==============================Original Headers============================== 3, 17 -- From jds451-at-psu.edu Sun Aug 20 09:51:57 2006 3, 17 -- Received: from webmail19.cac.psu.edu (webmail19.cac.psu.edu [128.118.1.205]) 3, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7KEpvZI004541 3, 17 -- for {microscopy-at-microscopy.com} ; Sun, 20 Aug 2006 09:51:57 -0500 3, 17 -- Received: (from webmail-at-localhost) 3, 17 -- by webmail19.cac.psu.edu (8.9.3/8.9.0) id KAA20369; 3, 17 -- Sun, 20 Aug 2006 10:51:56 -0400 (EDT) 3, 17 -- Date: Sun, 20 Aug 2006 10:51:56 -0400 (EDT) 3, 17 -- Message-Id: {200608201451.KAA20369-at-webmail19.cac.psu.edu} 3, 17 -- To: microscopy-at-microscopy.com 3, 17 -- Subject: Software: Montage 3, 17 -- From: "Jen Sloppy" {jds451-at-psu.edu} 3, 17 -- X-Mailer: Penn State WebMail 3, 17 -- X-Sender: jds451 3, 17 -- X-Originating-IP: 207.255.156.167 3, 17 -- MIME-Version: 1.0 3, 17 -- Content-Type: text/plain ==============================End of - Headers==============================
==============================Original Headers============================== 12, 35 -- From lazarz-at-mskcc.org Tue Aug 22 14:28:47 2006 12, 35 -- Received: from extmail.mskcc.org (mskresolve-9.mskcc.org [140.163.254.134]) 12, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MJSlVI015018 12, 35 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 14:28:47 -0500 12, 35 -- Received: from 140.163.141.235 by extmail.mskcc.org with ESMTP (ESMTP 12, 35 -- Relay); Tue, 22 Aug 2006 15:28:41 -0400 12, 35 -- X-Server-Uuid: 74E28B0A-64D9-4563-A4EB-AECFC40E1CE3 12, 35 -- Received: from SMSKPEXMBX6.MSKCC.ROOT.MSKCC.ORG ([140.163.141.233]) by 12, 35 -- smskpexsmtp2.MSKCC.ROOT.MSKCC.ORG with Microsoft 12, 35 -- SMTPSVC(5.0.2195.6713); Tue, 22 Aug 2006 15:28:41 -0400 12, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 12, 35 -- content-class: urn:content-classes:message 12, 35 -- MIME-Version: 1.0 12, 35 -- Subject: RE: [Microscopy] Software: Montage 12, 35 -- Date: Tue, 22 Aug 2006 15:28:41 -0400 12, 35 -- Message-ID: {0534480A8E1A0F4187DC6FFC3DDEECFF105D08-at-smskpexmbx6.mskcc.root.mskcc.org} 12, 35 -- X-MS-Has-Attach: 12, 35 -- X-MS-TNEF-Correlator: 12, 35 -- Thread-Topic: [Microscopy] Software: Montage 12, 35 -- Thread-Index: AcbEafXouMo6JtQqTTaLoK6w3FUoHwBtrb+m 12, 35 -- References: {200608201504.k7KF4Sea013592-at-ns.microscopy.com} 12, 35 -- From: LazarZ-at-mskcc.org 12, 35 -- To: microscopy-at-microscopy.com 12, 35 -- X-OriginalArrivalTime: 22 Aug 2006 19:28:41.0498 (UTC) 12, 35 -- FILETIME=[2CD477A0:01C6C621] 12, 35 -- X-TMWD-Spam-Summary: TS=20060822192842; SEV=2.0.1; DFV=A2006082207; 12, 35 -- IFV=2.0.4,4.0-8; RPD=4.00.0004; ENG=IBF; 12, 35 -- RPDID=303030312E30413031303230342E34344542353944432E303034392D412D; 12, 35 -- CAT=NONE; CON=NONE 12, 35 -- X-MMS-Spam-Filter-ID: A2006082207_4.00.0004_4.0-8 12, 35 -- X-WSS-ID: 68F585630YC1526875-01-01 12, 35 -- Content-Type: text/plain; 12, 35 -- charset=utf-8 12, 35 -- Content-Transfer-Encoding: 8bit 12, 35 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id k7MJSlVI015018 ==============================End of - Headers==============================
Listers, I had a response back on my Si microtoming tome from a biologist who wrote: "People like me (and Geoff) read this and go "OooKay ... what's a {111} plane and how would I know how I have such a thing aligned for anything, much less microtomy?"
Answer: Any Si semiconductor wafer you find will most likely have a {001} direction perpendicular to its surface. If it is a whole wafer there is probably a flat and a notch ground into the wafers edge at right angles to each other. The families of crystal {011} planes are oriented at 45 and 90 degrees with respect to each other. The flat is parallel to one of the {011} planes in the family. The notch is perpendicular to a second {011} plane at 90 degrees wrt to the flat. If it is a processed Si chip, look at the x, y coordinates defined on the surface of the chip. They are parallel to {011} planes. {111} planes are oriented at 35 and 90 degrees with respect to {011} planes. Try cleaving the wafer at 45 or 90 degrees wrt to the flat or notch. When you get a piece with a 35 degree slope wrt to the surface, the slope part will be a {111} face. OR, just hit the Si wafer with a hammer and look for a piece with shiny 35 degree sloped edges wrt the surface.
BUT, my biological friends, why are you making your heads hurt by reading this? The point of my original post was to explain why you won't want to fracture the edge of your diamond knife creating Si dust. Now you know that the dust particles will have 35 degree sloped sides! You can't microtome Si. Geoff, go read McCaffrey's paper on microcleaving. When Scott weighs in, he'll provide the reference, I hope.
Ron Anderson
==============================Original Headers============================== 5, 17 -- From randerson20-at-tampabay.rr.com Tue Aug 22 14:49:20 2006 5, 17 -- Received: from ms-smtp-01.tampabay.rr.com (ms-smtp-01.tampabay.rr.com [65.32.5.131]) 5, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MJnKuA025403 5, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 22 Aug 2006 14:49:20 -0500 5, 17 -- Received: from [127.0.0.1] (rrcs-24-73-73-214.se.biz.rr.com [24.73.73.214]) 5, 17 -- by ms-smtp-01.tampabay.rr.com (8.13.6/8.13.6) with ESMTP id k7MJnGaX023286 5, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 22 Aug 2006 15:49:19 -0400 (EDT) 5, 17 -- Message-ID: {44EB5FBA.2050008-at-tampabay.rr.com} 5, 17 -- Date: Tue, 22 Aug 2006 15:49:14 -0400 5, 17 -- From: Ronald Anderson {randerson20-at-tampabay.rr.com} 5, 17 -- User-Agent: Thunderbird 1.5.0.5 (Windows/20060719) 5, 17 -- MIME-Version: 1.0 5, 17 -- To: Listserver {Microscopy-at-Microscopy.Com} 5, 17 -- Subject: Microtoming Silicon 5, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 17 -- Content-Transfer-Encoding: 7bit 5, 17 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
I've never tried it on embedded and sectioned tissue, but wouldn't iodine work? It works well enough in fresh tissue, staining starch grains purple in light microscopy.
I do know that we've had a lot of trouble imaging tissue with starch granules in it, as they refract light, and mess up the signal in our confocal and tomography images. So polarized light might also work very well. Again I've never tried it.
I'm not sure if this helps, but there you go. Robin
--------------------------------- Robin Young, M.Sc. PhD Candidate Samuels and Haughn Labs Dept of Botany, UBC 6270 University Blvd. Vancouver, BC V6T 1Z4 fax: 604-822-6089
==============================Original Headers============================== 4, 27 -- From youngre-at-interchange.ubc.ca Tue Aug 22 15:04:55 2006 4, 27 -- Received: from mta4.mail-relay.ubc.ca (mta4.mail-relay.ubc.ca [137.82.45.8]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MK4s78003559 4, 27 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 15:04:55 -0500 4, 27 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 4, 27 -- by mta4.mail-relay.ubc.ca (8.12.11.20060308/8.12.11) with ESMTP id k7MK4icq006821 4, 27 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 13:04:44 -0700 (PDT) 4, 27 -- (envelope-from youngre-at-interchange.ubc.ca) 4, 27 -- Received: from [137.82.136.201] (robin.botany.ubc.ca [137.82.136.201]) 4, 27 -- by smtp.interchange.ubc.ca 4, 27 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 4, 27 -- with ESMTPSA id {0J4F00FD213W8U-at-smtp.interchange.ubc.ca} for 4, 27 -- microscopy-at-microscopy.com; Tue, 22 Aug 2006 13:04:44 -0700 (PDT) 4, 27 -- Date: Tue, 22 Aug 2006 13:04:44 -0700 4, 27 -- From: Robin Young {youngre-at-interchange.ubc.ca} 4, 27 -- Subject: Re: staining starch in sections 4, 27 -- To: microscopy-at-microscopy.com 4, 27 -- Message-id: {2EDB6CAF-30C7-4B9C-B0FB-1062C1634EB9-at-interchange.ubc.ca} 4, 27 -- MIME-version: 1.0 (Apple Message framework v752.2) 4, 27 -- X-Mailer: Apple Mail (2.752.2) 4, 27 -- Content-type: text/plain; format=flowed; delsp=yes; charset=US-ASCII 4, 27 -- Content-transfer-encoding: 7bit 4, 27 -- X-UBC-Scanned: Sophos PureMessage 4.7.1.128075, Antispam-Engine: 2.4.0.264935, Antispam-Data: 2006.8.22.115442 4, 27 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 4, 27 -- X-PerlMx-Spam: Probability=7%, Report=__C230066_P1_5 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_CRUFT 0 4, 27 -- X-Spam-Level: 4, 27 -- X-Spam-Flag: No ==============================End of - Headers==============================
Looking to digitize analog NTSC video tape (Hi-8 and VHS, so composite video, not S-video or component). Would like to stream the analog signal right in to digital format for later image analysis (so only need the video data no sound), and would like to work whole 60min tapes at a time. Since we’re looking to collect measurement data from video still images the higher the quality preserved the better. So looking for *specific* recommend ations as to the best method for doing this (specific as in model numbers).
Computer system (Windows PC) we have for this: has 1394, USB, 16x PCIe, x4 PCIe, 64-bit PCI, and 32-Bit PCI, with a RAID Array. Adobe Premiere reports system can sustain over 35MB/sec throughput.
Possible solutions:
1) A/D capture card, o.k., which one for running under Windows XP? Capturing composite NTSC video seems to be “old-tech” these days. Seemingly easy to find HDTV capture cards but not NTSC.
2) A/D capture “widgets” = Composite video in and IEEE 1394 output or USB? Seems like a very cost effective solution, can anyone recommend a model?
3) Using either a Digital8 camcorder or something like a Sony Digital8 Video Walkman to play the “tapes” out to 1394 and input the 1394 to the computer. (Note: Digital8 Players will play Hi-8 tapes). Will this work?
Video / Image Analysis: Once digitized the “video” will be broken up into digital stills (yes, 18 hours of still images at 29 frames / sec.). We actually only need 1 image from approximately every 4 seconds of video. Every 4 seconds the video image changes to one of 16 “views”. We will then sort the images into the individual 16 “views”. Each of the 16 “views” shows 7 developing seedlings. We will then analyze each of the 16 “views” (or sets of seedlings) for growth changes through time. Oh, yes after each set of 16 “views” the tape is “paused” for 10 mins, and then repeats the 16 view capture sequence again. So basically it is time lapse imaging, of 16 different subjects, all interleaved on one video stream.
And why collect data this way you ask? Because the data collection is all done automatically and remotely . . . From a minimum of 355 miles up in orbit on the International Space Station, on an automated growth and imaging system, which does not have room for 16 video cameras and video capture systems.
Any suggestions would be greatly appreciated.
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"A main-frame: The biggest PC peripheral available."
==============================Original Headers============================== 16, 24 -- From edelmare-at-muohio.edu Tue Aug 22 15:12:24 2006 16, 24 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 16, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MKCNVa013909 16, 24 -- for {microscopy-at-Microscopy.com} ; Tue, 22 Aug 2006 15:12:24 -0500 16, 24 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 16, 24 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id k7MKCBHt014555 16, 24 -- for {microscopy-at-Microscopy.com} ; Tue, 22 Aug 2006 16:12:12 -0400 16, 24 -- Received: from emf03 ([134.53.14.97]) 16, 24 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id k7MKC9EJ014949 16, 24 -- for {microscopy-at-Microscopy.com} ; Tue, 22 Aug 2006 16:12:09 -0400 16, 24 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 16, 24 -- To: microscopy-at-Microscopy.com 16, 24 -- Date: Tue, 22 Aug 2006 16:14:11 -0400 16, 24 -- MIME-Version: 1.0 16, 24 -- Content-type: text/plain; charset=ISO-8859-1 16, 24 -- Subject: Looking for some video capture recommendations 16, 24 -- Message-ID: {44EB2D53.3411.1471D88D-at-localhost} 16, 24 -- Priority: normal 16, 24 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 16, 24 -- X-Real-ConnectIP: 134.53.14.97 16, 24 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 16, 24 -- X-Scanned-By: MIMEDefang 2.52 on 134.53.6.11 16, 24 -- Content-Transfer-Encoding: 8bit 16, 24 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id k7MKCNVa013909 ==============================End of - Headers==============================
I've used Iodine-Potassium Iodide solution (dissolve 2g of potassium iodide in 100 ml water then dissolve 0.2g of iodine in this).
Ian
Ian Hallett Microscopy HortResearch Mt Albert Research Centre Private Bag 92 169, Mt Albert Auckland, New Zealand +64-9-815 4200 ext 7002
-----Original Message----- X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] Sent: Wednesday, 23 August 2006 2:26 a.m. To: Ian Hallett
Greetings, Can anyone tell me a reliable method to stain starch grains in sections of plant tissue embedded in Spurr's resin? Also useful to know whether polarized light would be suitable. I hope this is easy and I get lots of replies!
As ever, Tobias -- _ ____ __ ____ / \ / / \ / \ \ Tobias I. Baskin / / / / \ \ \ Biology Department /_ / __ /__ \ \ \__ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / / \ \ \ Amherst, MA, 01003 / / ___ / \ \__/ \ ____ http://www.bio.umass.edu/biology/baskin/ Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
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==============================Original Headers============================== 15, 30 -- From IHallett-at-hortresearch.co.nz Tue Aug 22 15:58:34 2006 15, 30 -- Received: from hortresearch.co.nz (mscan.hortresearch.co.nz [202.36.134.15]) 15, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MKwXec024715 15, 30 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 15:58:34 -0500 15, 30 -- Received: from aklexf01.hort.net.nz ([10.16.1.14]) by hortresearch.co.nz 15, 30 -- with HortResearch; Wed, 23 Aug 2006 09:09:55 +1200 15, 30 -- Received: from AKLEXB01.hort.net.nz ([10.16.1.15]) by aklexf01.hort.net.nz 15, 30 -- with Microsoft SMTPSVC(6.0.3790.1830); Wed, 23 Aug 2006 08:58:00 +1200 15, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 15, 30 -- Content-class: urn:content-classes:message 15, 30 -- MIME-Version: 1.0 15, 30 -- Content-Type: text/plain; 15, 30 -- charset=us-ascii 15, 30 -- Subject: RE: [Microscopy] staining starch in sections 15, 30 -- Date: Wed, 23 Aug 2006 08:57:59 +1200 15, 30 -- Message-ID: {D3BAD63C088F3C48AEB385E881359F870214C10A-at-AKLEXB01.hort.net.nz} 15, 30 -- In-Reply-To: {200608221425.k7MEPxFe020693-at-ns.microscopy.com} 15, 30 -- X-MS-Has-Attach: 15, 30 -- X-MS-TNEF-Correlator: 15, 30 -- Thread-Topic: [Microscopy] staining starch in sections 15, 30 -- Thread-Index: AcbF9uX7+gwr9oxdQyCAVrJmBhwvjQANh36A 15, 30 -- From: "Ian Hallett" {IHallett-at-hortresearch.co.nz} 15, 30 -- To: {microscopy-at-microscopy.com} 15, 30 -- X-OriginalArrivalTime: 22 Aug 2006 20:58:00.0040 (UTC) 15, 30 -- FILETIME=[A6C51680:01C6C62D] 15, 30 -- X-imss-version: 2.042 15, 30 -- X-imss-result: Passed 15, 30 -- X-imss-approveListMatch: *-at-hortresearch.co.nz 15, 30 -- Content-Transfer-Encoding: 8bit 15, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k7MKwXec024715 ==============================End of - Headers==============================
Have you considered the Thièry method, using periodate oxidation of vicinal hydroxyls in carbohydrates, reacting resulting aldehydes with thiocarbohydrazide or thiosemicarbazide followed by silver proteinate? It may not be the easiest method, though, but it works really well.
I don't have the original reference (1964 I believe), but it is a.o. described in JHC Volume 33, Issue 10, pp. 1007-1014, 1985
Jan Leunissen
Research Advisor Electron Microcopy Dept Anatomy / Structural Biology http://anatomy.otago.ac.nz/
==============================Original Headers============================== 9, 18 -- From leunissen-at-aurion.nl Tue Aug 22 16:05:30 2006 9, 18 -- Received: from fep04.xtra.co.nz (fep04.xtra.co.nz [210.54.141.242]) 9, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7ML5TnC002729 9, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 16:05:30 -0500 9, 18 -- Received: from [192.168.1.50] (really [222.152.176.211]) 9, 18 -- by fep04.xtra.co.nz with ESMTP 9, 18 -- id {20060822210523.KTBB6771.fep04.xtra.co.nz-at-[192.168.1.50]} 9, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Aug 2006 09:05:23 +1200 9, 18 -- Mime-Version: 1.0 (Apple Message framework v752.2) 9, 18 -- Message-Id: {C1FD8ECD-C854-4933-AA03-0FE421FB6BC6-at-aurion.nl} 9, 18 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 9, 18 -- To: Microscopy-at-microscopy.com 9, 18 -- From: Jan Leunissen {leunissen-at-aurion.nl} 9, 18 -- Subject: Re: [Microscopy] staining starch in sections 9, 18 -- Date: Wed, 23 Aug 2006 09:05:22 +1200 9, 18 -- X-Mailer: Apple Mail (2.752.2) 9, 18 -- Content-Transfer-Encoding: 8bit 9, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k7ML5TnC002729 ==============================End of - Headers==============================
Iodine would surely stain starch molecules. Many years ago, I used iodine to stain blends of polypropylene and ethylene vinyl alcohol (EVOH) for analysis by SEM and TEM. The iodine did provide excellent initial selective contrast for the EVOH. Unfortunately, iodine is volatile in the vacuum of the microscope. The iodine bound by the EVOH dissipated sufficiently rapidly that I saw a significant reduction in contrast during the microscopy session.
A better stain for examination of starch in electron microscopes probably osmium tetroxide. Osmium binds the hydroxyl sites irreversibly, thus providing excellent heavy metal contrast in the preparation.
Good luck,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
youngre-at-interc hange.ubc.ca To gary.m.brown-at-exxonmobil.com 08/22/06 03:07 cc PM Subject [Microscopy] Re: staining starch in Please respond sections to youngre-at-interc hange.ubc.ca
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I've never tried it on embedded and sectioned tissue, but wouldn't iodine work? It works well enough in fresh tissue, staining starch grains purple in light microscopy.
I do know that we've had a lot of trouble imaging tissue with starch granules in it, as they refract light, and mess up the signal in our confocal and tomography images. So polarized light might also work very well. Again I've never tried it.
I'm not sure if this helps, but there you go. Robin
--------------------------------- Robin Young, M.Sc. PhD Candidate Samuels and Haughn Labs Dept of Botany, UBC 6270 University Blvd. Vancouver, BC V6T 1Z4 fax: 604-822-6089
==============================Original Headers============================== 4, 27 -- From youngre-at-interchange.ubc.ca Tue Aug 22 15:04:55 2006 4, 27 -- Received: from mta4.mail-relay.ubc.ca (mta4.mail-relay.ubc.ca [137.82.45.8]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MK4s78003559 4, 27 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 15:04:55 -0500 4, 27 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 4, 27 -- by mta4.mail-relay.ubc.ca (8.12.11.20060308/8.12.11) with ESMTP id k7MK4icq006821 4, 27 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 13:04:44 -0700 (PDT) 4, 27 -- (envelope-from youngre-at-interchange.ubc.ca) 4, 27 -- Received: from [137.82.136.201] (robin.botany.ubc.ca [137.82.136.201]) 4, 27 -- by smtp.interchange.ubc.ca 4, 27 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 4, 27 -- with ESMTPSA id {0J4F00FD213W8U-at-smtp.interchange.ubc.ca} for 4, 27 -- microscopy-at-microscopy.com; Tue, 22 Aug 2006 13:04:44 -0700 (PDT) 4, 27 -- Date: Tue, 22 Aug 2006 13:04:44 -0700 4, 27 -- From: Robin Young {youngre-at-interchange.ubc.ca} 4, 27 -- Subject: Re: staining starch in sections 4, 27 -- To: microscopy-at-microscopy.com 4, 27 -- Message-id: {2EDB6CAF-30C7-4B9C-B0FB-1062C1634EB9-at-interchange.ubc.ca} 4, 27 -- MIME-version: 1.0 (Apple Message framework v752.2) 4, 27 -- X-Mailer: Apple Mail (2.752.2) 4, 27 -- Content-type: text/plain; format=flowed; delsp=yes; charset=US-ASCII 4, 27 -- Content-transfer-encoding: 7bit 4, 27 -- X-UBC-Scanned: Sophos PureMessage 4.7.1.128075, Antispam-Engine: 2.4.0.264935, Antispam-Data: 2006.8.22.115442 4, 27 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 4, 27 -- X-PerlMx-Spam: Probability=7%, Report=__C230066_P1_5 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_CRUFT 0 4, 27 -- X-Spam-Level: 4, 27 -- X-Spam-Flag: No ==============================End of - Headers==============================
==============================Original Headers============================== 23, 20 -- From gary.m.brown-at-exxonmobil.com Tue Aug 22 16:08:45 2006 23, 20 -- Received: from hoespc02.exxonmobil.com (hoespc02.exxonmobil.com [192.67.48.39]) 23, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7ML8iET011132 23, 20 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 16:08:45 -0500 23, 20 -- Received: from hounmg03.NA.XOM.COM (hounmg03.na.xom.com [158.35.101.41]) 23, 20 -- by hoespc02.exxonmobil.com (Switch-3.1.7/Switch-3.1.7) with ESMTP id k7ML8dQP027536; 23, 20 -- Tue, 22 Aug 2006 16:08:39 -0500 (CDT) 23, 20 -- In-Reply-To: {200608222007.k7MK7Ct3007202-at-ns.microscopy.com} 23, 20 -- Subject: Re: [Microscopy] Re: staining starch in sections 23, 20 -- Importance: 23, 20 -- To: youngre-at-interchange.ubc.ca, microscopy-at-microscopy.com 23, 20 -- Cc: donald.a.winesett-at-exxonmobil.com 23, 20 -- X-Mailer: Lotus Notes Release 6.5.2 June 01, 2004 23, 20 -- Message-ID: {OF254CE6BC.DC284982-ON862571D2.0072DBD2-862571D2.0074255C-at-exxonmobil.com} 23, 20 -- From: gary.m.brown-at-exxonmobil.com 23, 20 -- Date: Tue, 22 Aug 2006 16:08:37 -0500 23, 20 -- X-MIMETrack: Serialize by Router on Hounmg03.na.xom.com/S/ExxonMobil(652FP1HF193|March 23, 20 -- 02, 2006) at 08/22/2006 04:08:50 PM 23, 20 -- MIME-Version: 1.0 23, 20 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Thank you, Ron and Henk, for the plug. Sorry, I was on vacation or else I would have answered this.
I'd like to address the MicroCleave(TM) preparation at the end of this discussion so that it is closest to the disclaimer that I am going to put on it.
SDW on Microtoming: I've seen some images that Tom Malis collected of microtomed Si samples from different sources. True, you get a lot of mostly dust as Ron says, but apparently when you hit it using the right conditions, you can get little cracked pieces that compose the ribbon that have the film intact on them. If you are interested in only the film, you have a chance of getting what you need. However, for the most part, there are other methods for making Si cross sections that are much easier and much more likely for producing a usable sample.
SDW on Ion Milling: I have worked with thin Au-Ge-Ni multi-layers on GaAs in the past and you do have to worry about heating the sample as you indicate. You can use superglue in place of wax; you just have to be patient to get rid of the glue. If you ion mill your sample, you really have to worry about heating the sample during ion milling. The answer there is to use low angle, low energy, and it wouldn't hurt to cool the sample with a liquid nitrogen cooled stage. Since it is a competitor's product, I will not mention the name, but there is one popular ion mill on the market that you have to be particularly careful with. This ion mill does not have a sample holder that has a large heat sink and has a relatively poor thermal path through the holder. If you have this type of ion mill, you must significantly de-tune the ion guns or you can heat your samples very high even at low milling angles. Unfortunately, this "de-tuning" by lowering the ion energy and limiting the ion beam current will significantly impact the ion milling rate. I have melted glass cross sections with this ion mill. I have used other ion mills with the glass and for the most part, they are better in terms of not heating the glass samples simply because the stages dissipate the heat better, but you still have to be careful. In all of the conventional ion mills, i.e. non low-energy types, the beam current drops to essentially zero below about 3 kV. There are a number of studies that have been done concerning the heating of samples in ion mills. You can contact me off-line to talk about the low energy guns in the ion mills that we sell.
SDW on MicroCleaving: OK, let me sound off for the MicroCleave(TM), also known as the Small Angle Cleavage Technique. (You can even consider this coming from Igor Slobinsky and Dr. Cleavinstein so maybe I won't get in trouble for being too commercial.) I thought that this technique is the neatest thing since sliced bread (or sliced samples) when I started using it and I still do. This technique produces the best cross section samples of any available technique because there is simply no ion damage produced. In fact, a major disadvantage of these types of samples is that if the samples are fully crystalline and you are using a field emission gun equipped microscope, then there is no amorphous regions to help the microscopist focus and stigmate with. I suspect that with the new C(s)-corrected machines, more and more people will be discovering this technique.
For the metallization samples, the MicroCleave(TM) technique will work as long as the layers are very thin. Generally, they should be less than 1000A. They also must be fully adherent to the substrate and to the other layers. For as-deposited Au-Ge-Ni on GaAs samples with e-beam evaporated Ge layers that I mentioned above, I found that the Ge layers were weak and would split within the layer during the cleave. I've never looked at Ge layers put down by other methods, so I don't know whether that is a universal trait of Ge layers or not. If we heat treated them to alloy the layers, I had no such problem. Even a slight anneal where there was no apparent mixing of the layer materials would help prevent the Ge layer from splitting. I suspect that we might have been getting a little densification with the slight anneal. What happens with these samples during the procedure is that the thinnest parts of the samples would consist of any layers below the Ge and about half the Ge layer. Further back, all of the layers would be intact, but thicker. I can send a copy of a presentation on the MicroCleave technique that John McCaffrey and I did in "Cleave-Land" at an M&M meeting in 1997 to anyone that wants one. There are two places in the presentation that addresses temperature sensitive samples. First, as you point out, use superglue and then wait for the superglue to dissolve when finished instead of using the low temperature melting wax. The other place is that for the mounting epoxy, you need a viscous and slow setting epoxy to mount the samples onto the specially bent Veco 2x1 tabbed grids. Normally, Epoxy Technology's H-22 silver filled epoxy is used, but that requires heat to set. The 90 minute Super strength type of two part epoxies work well, but you have to give them a full day to set up. They are also nonconductive, but that didn't seem to be much of a problem. I've tried a number of different brands, and they seemed to work, but they have to be the 90 minute working time ones. Other epoxies flowed too much and did not stay in position to hold the sample. I also experimented with fillers for the epoxy with no success at all. There is also a temperature spike when an epoxy cures. When I used this on temperature sensitive samples, I tried to minimize the amount of epoxy on the grids so that the grid could act as a heat sink for the exothermic reaction and the samples would experience the minimum of a thermal excursion.
One other point of caution for about Si samples prepared this way. The samples can become very very thin and basically disappear into nothingness at the edges. I had a pulsed laser deposited diamond like carbon film that was deposited onto silicon and prepared this way that was too thin at the edges to do electron energy loss spectroscopy on and get sufficient signal! That's thin! What it means is that the samples prepared this way can be heated by a focused beam because there isn't enough thermal path to extract heat from the exposed area.
Here is a list of the references that I have. The "Update" paper by John and me also has an accompanying presentation that I can send to people in a PDF format that I can send to anyone that is interested. This presentation and the MRS paper by us have a pictorial outline of the technique in it. There are a lot of hands-on tips and tricks and details that we tried to put into the paper so that someone could try the technique without reinventing the wheel.
1) "Small Angle Cleavage of Semiconductors for TEM", J.P. McCaffrey, Ultramicroscopy 38, 1991, pp:149-157 2) "TEM Samples of Semiconductors Prepared by a Small Angle Cleavage Technique", J.P. McCaffrey, Mat. Res. Soc. Symp. Proc., Vol.254, 1992, pp:109-120 3) John P. McCaffrey, Microscopy Research and Technique, 24, (1993) 180. 4) "A Simplified Method for Modifying TEM Copper Grids For Use with the Small Angle Cleavage Technique", Scott D. Walck, Microscopy Today, (96-4), (1996). 5) The Small Angle Cleavage Technique Applied to Coatings and Thin Films, S. D. Walck and J. P. McCaffrey, Thin Solid Films, 308-309, (1997) 399. 6) "The Small Angle Cleavage Technique: An Update", Scott D. Walck, John P. McCaffrey, Mat.Res.Soc.Symp.Proc., Vol.480,1997,pp:149-170
OK, here is the disclaimer that I hope will keep me out of trouble. South Bay Technology Inc. makes and sells the Microcleave(TM) Kit. SBT also sells the Technoorg-Linda line of ion mills with the low energy ion gun.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: randerson20-at-tampabay.rr.com [mailto:randerson20-at-tampabay.rr.com] Sent: Tuesday, August 22, 2006 11:20 AM To: Walck-at-SouthBayTech.com
Geoff,
No you can't "cut" Si with a diamond knife. Si cleaves very easily. When the knife touches the Si, the Si cleaves along one of its preferred {111} crystallographic cleaving planes. That might be fine if you orient the Si such that a Si {111} crystallographic direction is perpendicular to the block face. Then if you are VERY lucky, you MIGHT get a cleaved piece of Si oriented with {111} faces (at the risk of your knife). Any article that I've seen that claims to have microtomed Si has shown lots of pictures of tiny {111} Si sections.
Assuming that you are successful, so what! There is no, or very little interest, in anything in the semiconductor world or in potentially oriented thin films deposited on Si, that is best displayed on top of a Si cross section with a {111} block face orientation. The angles are wrong.
Si wafers in semiconductor processing are almost all with (or very close to) a Si {001} direction perpendicular to the Si wafer surface. Devices fabricated in, or patterns deposited on, these wafers are not randomly oriented. They are almost always aligned with {011} planes perpendicular to the Si surface, i.e. the x, y orientations of the devices and patterns are parallel to {011} directions in the Si surface. Cross sections of interest for TEM or SEM analysis are oriented such that the microscope looks down on a {011} "block face." If you orient the specimen's block face so as to attempt to cut {011} cross sections, the diamond knife will touch the Si and immediately propagate a cleave down the nearest {111} plane despite the orientation of the block. As the specimen in the block is constrained by the epoxy holding it, the result is a flake of Si dust from just the surface. When the knife comes around again you get more dust.
Using a microtome to make Si {011}cross sections is like using a screw driver to drive a nail.
Instead of fighting the fact that Si cleaves, why not exploit this fact and prepare really great thin specimens by the microcleaving technique pioneered by McCaffrey and Igor. I'll copy them and invite them to expand on this suggestion. They'll point out that you can make nice thin sections of thin films on Si with a minimum of tools and at room temperature. There is a videotape that shows how to do it.
Ron Anderson
Geoffrey_Williams-at-brown.edu wrote: } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } I do not know exactly what I can divulge, but they have tried tripod polishing, and ion-milling but I am told they elevate the temperature and the layers need to be prepped and kept at room temps until imaging. } } Does that say enough? I still feel it's a bit irrelevant to the initial question. } } Is it possible to section a Si wafer? Am I correct in being skeptical? Or should I just kill a $4k knife or try an ancient already messed up and questionably anchored diamond in the attempt? } } I just figured someone out there might be able to share a bit of practical experience. } } Thanks! } } Geoff Williams } Leduc Bioimaging Facility Manager } Brown University } } http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/ } } } } ==============================Original } Headers============================== } 8, 29 -- From Geoffrey_Williams-at-brown.edu Tue Aug 22 10:59:55 2006 8, } 29 -- Received: from draco.services.brown.edu (draco.services.brown.edu [128.148.106.172]) } 8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MFxtQK026422 } 8, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 10:59:55 -0500 } 8, 29 -- Received: from ex-gateway1-out.AD.Brown.Edu (ex-gateway1.ad.brown.edu [128.148.21.51]) } 8, 29 -- by draco.services.brown.edu (Switch-3.1.8/Switch-3.1.7/) with ESMTP id k7MFxs7W006189 } 8, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 11:59:54 -0400 (EDT) } 8, 29 -- Received: from mail1.AD.Brown.Edu ([128.148.21.30]) by ex-gateway1-out.AD.Brown.Edu with Microsoft SMTPSVC(6.0.3790.1830); } 8, 29 -- Tue, 22 Aug 2006 11:59:54 -0400 } 8, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 8, 29 } -- Content-class: urn:content-classes:message 8, 29 -- MIME-Version: } 1.0 8, 29 -- Content-Type: text/plain; } 8, 29 -- charset="iso-8859-1" } 8, 29 -- Subject: Sectioning Si, clarification 8, 29 -- Date: Tue, 22 } Aug 2006 11:59:53 -0400 8, 29 -- Message-ID: } {A1A84D541C161C4C988B4E0FB38F5A0F047F6793-at-MAIL1.AD.Brown.Edu} } 8, 29 -- X-MS-Has-Attach: } 8, 29 -- X-MS-TNEF-Correlator: } 8, 29 -- Thread-Topic: Sectioning Si, clarification 8, 29 -- } Thread-Index: AcbGBAF0V914xLqeQPmnA7STgTxe2Q== 8, 29 -- From: } "Williams, Geoffrey" {Geoffrey_Williams-at-brown.edu} 8, 29 -- To: } {Microscopy-at-microscopy.com} 8, 29 -- X-OriginalArrivalTime: 22 Aug } 2006 15:59:54.0245 (UTC) FILETIME=[02015B50:01C6C604] 8, 29 -- } X-Brown-Proofpoint: Not Infected 8, 29 -- X-Proofpoint-Spam-Details: } rule=notspam policy=default score=0 mlx=-1 adultscore=0 adjust=0 } reason=safe engine=3.1.0-0608080000 definitions=main-0608220003 8, 29 } -- X-Brown-MailScanner-SpamCheck: not spam, rule=notspam } policy=default score=0 mlx=-1 adultscore=0 adjust=0 reason=safe } engine=3.1.0-0608080000 definitions=main-0608220003 8, 29 -- } Content-Transfer-Encoding: 8bit 8, 29 -- X-MIME-Autoconverted: from } quoted-printable to 8bit by ns.microscopy.com id k7MFxtQK026422 } ==============================End of - } Headers============================== } } }
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==============================Original Headers============================== 29, 22 -- From walck-at-southbaytech.com Tue Aug 22 18:18:57 2006 29, 22 -- Received: from ylpvm43.prodigy.net (ylpvm43-ext.prodigy.net [207.115.57.74]) 29, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7MNIv4K025370 29, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 18:18:57 -0500 29, 22 -- X-ORBL: [64.169.193.90] 29, 22 -- Received: from dynamicbl8uno3 (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90]) 29, 22 -- by ylpvm43.prodigy.net (8.13.7 out spool5000 dk/8.13.7) with ESMTP id k7MNIsbi030444; 29, 22 -- Tue, 22 Aug 2006 19:18:55 -0400 29, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} 29, 22 -- To: {Microscopy-at-microscopy.com} 29, 22 -- Cc: {randerson20-at-tampabay.rr.com} , "Henk Colijn" {colijn.1-at-osu.edu} 29, 22 -- Subject: RE: [Microscopy] Re: Sectioning Si, clarification 29, 22 -- Date: Tue, 22 Aug 2006 16:19:36 -0700 29, 22 -- Message-ID: {00a301c6c641$6f4255b0$7801a8c0-at-dynamicbl8uno3} 29, 22 -- MIME-Version: 1.0 29, 22 -- Content-Type: text/plain; 29, 22 -- charset="us-ascii" 29, 22 -- Content-Transfer-Encoding: 7bit 29, 22 -- X-Mailer: Microsoft Office Outlook 11 29, 22 -- In-Reply-To: {200608221820.k7MIKOHm021597-at-ns.microscopy.com} 29, 22 -- Thread-Index: AcbGF6KHgJf2sp57RhCWrZ22d+K1vQACaacw 29, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
If you don't want 16-bit TIFF, why not just save it as 8-bits? SaveAs/Options/Save16-bitAs8-bit.
Save the 16-bit as the original and save the 8-bit from analySIS as the 8-bit version. Dynamic range will be reduced but pixel loss will be reduced.
gary g.
At 08:24 AM 8/22/2006, you wrote:
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Not being a TEM person, I'm exactly sure what you need to have done.
Si wafers are easy to cut using a carbide tip pens available from Home Depot (~$6). You can buy pre-cut wafers from Ted Pella but you will get a lot of little die--probably more than you need.
The easiest way to cut coated Si material is to cleave it. This is perfect for cross section analysis.
If you don't get the Si material's lattice lined up properly, trying any variable cutting method will yield a pile of Si dust. You must be parallel or perpendicular to the wafer flat.
gary g.
At 08:33 AM 8/22/2006, you wrote:
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==============================Original Headers============================== 12, 22 -- From gary-at-gaugler.com Tue Aug 22 18:58:22 2006 12, 22 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k7MNwLpA013869 12, 22 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 18:58:22 -0500 12, 22 -- Received: (qmail 26517 invoked from network); 22 Aug 2006 16:58:20 -0700 12, 22 -- Received: by simscan 1.1.0 ppid: 26512, pid: 26515, t: 0.2015s 12, 22 -- scanners: regex: 1.1.0 attach: 1.1.0 12, 22 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 12, 22 -- by qsmtp1 with SMTP; 22 Aug 2006 16:58:20 -0700 12, 22 -- Message-Id: {7.0.1.0.2.20060822165222.0253aa78-at-gaugler.com} 12, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 12, 22 -- Date: Tue, 22 Aug 2006 16:58:21 -0700 12, 22 -- To: Geoffrey_Williams-at-brown.edu 12, 22 -- From: Gary Gaugler {gary-at-gaugler.com} 12, 22 -- Subject: Re: [Microscopy] Sectioning Si Wafers 12, 22 -- Cc: MSA listserver {microscopy-at-microscopy.com} 12, 22 -- In-Reply-To: {200608221533.k7MFXQG0008602-at-ns.microscopy.com} 12, 22 -- References: {200608221533.k7MFXQG0008602-at-ns.microscopy.com} 12, 22 -- Mime-Version: 1.0 12, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 12, 22 -- Content-Transfer-Encoding: 8bit 12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k7MNwLpA013869 ==============================End of - Headers==============================
I have a montage assembler that is based on Windows Explorer. The individual images must be of consistent size, have overlap between adjacent images and not exceed total available memory.
If you wish to see a sample, can you send me some files? 10x10 (100) images ought to show if this program will work.
The data so far has been collected on a Prior automated stage.
There is no user manual and the software could be free. But it should be tested to be sure that it will work for your application.
gary g.
At 07:55 AM 8/20/2006, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Tue Aug 22 19:04:39 2006 11, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id k7N04clj024143 11, 20 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 19:04:39 -0500 11, 20 -- Received: (qmail 29961 invoked from network); 22 Aug 2006 17:04:37 -0700 11, 20 -- Received: by simscan 1.1.0 ppid: 29958, pid: 29959, t: 0.1654s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 20 -- by qsmtp1 with SMTP; 22 Aug 2006 17:04:37 -0700 11, 20 -- Message-Id: {7.0.1.0.2.20060822165931.02559ba8-at-gaugler.com} 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 11, 20 -- Date: Tue, 22 Aug 2006 17:04:38 -0700 11, 20 -- To: jds451-at-psu.edu 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] Software: Montage 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200608201455.k7KEtlmP007749-at-ns.microscopy.com} 11, 20 -- References: {200608201455.k7KEtlmP007749-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
On Aug 22, 2006, at 11:46, voyles-at-engr.wisc.edu wrote:
} There is also a potentially serious drawback to the Photoshop } procedure } you suggest. Photoshop discards the original grey-scale values of the } image when you use "Auto Levels", which breaks the quantitative } connection between the grey-scale value in the image and the absolute } number of electrons that struck the detector. This will often make } quantitative analysis of the electron image or diffraction pattern } impossible and makes it difficult to even judge the relative average } brightness of two different images to guess, for example, the sample } thickness.
This is true. However, it is trivial to rectify the problem that he's having, which is that his system is saving 12-bit TIFFs. Simply use the Convolve function in Photoshop to multiply the image by 16 uniformly. This preserves the quantitative connection while allowing you to visually observe the image. (One notes that there was a very simple tutorial for this in Microscopy Today about two years ago... *g*)
} Software developed for photographs and desktop } publishing is not generally the best choice for dealing with } scientific } data.
I disagree with this statement. You can be perfectly quantitative in Photoshop, and in fact many people are (see all the Fovea Pro users, for example.) The issue at hand is one of needing to understand what one is doing to an image. But that issue is true no matter what software you're using. You can screw up data in ImagePro just as easily as you can in Photoshop.
Brent
-- Brent Neal Research Physicist Milliken Research Corporation
==============================Original Headers============================== 16, 27 -- From brentn-at-gmail.com Tue Aug 22 21:00:32 2006 16, 27 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.232]) 16, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id k7N20WNQ004147 16, 27 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 21:00:32 -0500 16, 27 -- Received: by wx-out-0506.google.com with SMTP id t11so2335081wxc 16, 27 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2006 19:00:32 -0700 (PDT) 16, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 16, 27 -- s=beta; d=gmail.com; 16, 27 -- h=received:mime-version:in-reply-to:references:content-type:message-id:content-transfer-encoding:from:subject:date:to:x-mailer:sender; 16, 27 -- b=bwBnQXHztGvZI8EEIWyvov8Ee15ACIWwMiqFhBFGAYTv5MRUOo4JrMAV1cfQmQFYZRdFGFOou/m5yfDCMpap52a0d9rYgyqs37+s94L4mUAp8mGdNm6+YG3F9XXcSmpXwxQyVGHePVwbBJ8K8MoDgFiV1y5lUy8IH4LRvAPpnnU= 16, 27 -- Received: by 10.70.90.18 with SMTP id n18mr12734220wxb; 16, 27 -- Tue, 22 Aug 2006 19:00:32 -0700 (PDT) 16, 27 -- Received: from ?192.168.123.152? ( [66.169.84.242]) 16, 27 -- by mx.gmail.com with ESMTP id 28sm254642wrl.2006.08.22.19.00.31; 16, 27 -- Tue, 22 Aug 2006 19:00:31 -0700 (PDT) 16, 27 -- Mime-Version: 1.0 (Apple Message framework v749.3) 16, 27 -- In-Reply-To: {200608221546.k7MFk6HI018268-at-ns.microscopy.com} 16, 27 -- References: {200608221546.k7MFk6HI018268-at-ns.microscopy.com} 16, 27 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 16, 27 -- Message-Id: {10DC6380-E144-47A9-B6DB-45921A38D022-at-freeshell.org} 16, 27 -- Content-Transfer-Encoding: 7bit 16, 27 -- From: Brent Neal {brentn-at-freeshell.org} 16, 27 -- Subject: Re: [Microscopy] Re: 16bit TIFF images in Photoshop 16, 27 -- Date: Tue, 22 Aug 2006 22:00:28 -0400 16, 27 -- To: microscopy-at-microscopy.com 16, 27 -- X-Mailer: Apple Mail (2.749.3) 16, 27 -- Sender: Brent Neal {brentn-at-gmail.com} ==============================End of - Headers==============================
Although this remark is right when one considers the auto-contrast option, I don't think you change the relative values of the pixels by adjusting the levels. As was described in another answer, reading this format in Photoshop compresses the values of the left side of the histogram. To recover a "normal" histogram, one has just to adjust the limits to exclude all the pixels with a 0 val