Here is the November 2006 Microscopy Today table of contents. I will close the subscription list for this issue on Monday, Nov 6th, 2006.
Microscopists in North America and MSA members anywhere can subscribe for free. Anyone else may subscribe for US$50 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com Thank you.
Ron Anderson, Editor ================================ Microscopes in Art Galleries? Stephen W. Carmichael, Mayo Clinic
Recent Advances in High-Speed Orientation Mapping Matthew M. Nowell1, Martina Chui-Sabourin2, and John O. Carpenter1 1 EDAX-TSL, Draper, UT, 2 EDAX-TSL, Mahwah, NJ
Diffracted Light Contrast: Improving the Resolution of a Basic Light Microscope by an Order of Magnitude W. Barry Piekos, Yale University, New Haven, CT
Nature’s Engineering Marvels: the Structure and Chemistry of a Butterfly Wing V.S. Smentkowski, S.G. Ostrowski, E.J. Olson, J. Cournoyer, K. Dovidenko, R.A. Potyrailo, General Electric Niskayuna, NY
Integrating High Resolution Light Microscopy and Real Time Observation of Fluorescent Labels Thomas A. Hasling, Aetos Technologies, Inc., Auburn, AL
Surface Rippling & Ion Etch Yields of Diamond Using a Focused Ion Beam: With or Without Enhanced-Chemistry, Aspect Ratio Regulates Ion Etching W. J. MoberlyChan, T. E. Felter, & M. A. Wall, Lawrence Livermore National Lab., Livermore, CA
Internet-Based Administration of Shared Instruments with Facility Online Manager Shu-You Li and Vinayak P. Dravid, Northwestern University, Evanston, IL
Andrew Paul Leonard: Capturing the Cover of Time Magazine Lise Millay Stevens, MA
Streamlining the Modern Lab Radhika Subramanian, Cornet Technology, Inc., Springfield, VA
Intermediate Magnification Imaging System for Whole Organs/Organisms Richard W. Cole, Carmen A. Mannella, Christian Renken, and James N. Turner, Wadsworth Center, Albany, NY
The NEST Laboratory: The Art of a Multi-User Facility Scott Streiker and Rachel Smith, University of Dayton, Dayton, OH
Butyl-methyl-methacrylate for Immunocytochemistry Through the Light Microscope Tobias I. Baskin, University of Massachusetts, Amherst, MA
How To Stick Loosely Adherent Cells To Glass Slides Martin Spitaler, Imperial College, London, UK
Just Say NO to Microtoming Silicon! Ron Anderson, Microscopy Today, Largo, FL
Industry News
Netnotes SPECIMEN PREPARATION - ventricle embedding problem SPECIMEN PREPARATION - sample preparation polymer blend SPECIMEN PREPARATION - Staining starch in sections SPECIMEN PREPARATION - staining methods SPECIMEN PREPARATION - Thiocarbohydrazide SPECIMEN PREPARATION - cotton fibers IMMUNOCYTOCHEMISTRY - Immunogold labeling & SEM IMMUNOCYTOCHEMISTRY – testing colloidal gold IMAGE ANALYSIS – object size EM - Venting EM Chambers TEM – electron diffraction TEM – Nickel grids & EDX SEM – Back scattered electrons and edge effects SEM – Backscattered electron images
Index of Advertisers
==============================Original Headers============================== 21, 18 -- From microscopytoday-at-tampabay.rr.com Wed Nov 1 12:34:25 2006 21, 18 -- Received: from ms-smtp-02.tampabay.rr.com (ms-smtp-02.tampabay.rr.com [65.32.5.132]) 21, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA1IYO6A024688 21, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 1 Nov 2006 12:34:25 -0600 21, 18 -- Received: from [127.0.0.1] (rrcs-24-73-73-214.se.biz.rr.com [24.73.73.214]) 21, 18 -- by ms-smtp-02.tampabay.rr.com (8.13.6/8.13.6) with ESMTP id kA1IYK4Y002917; 21, 18 -- Wed, 1 Nov 2006 13:34:22 -0500 (EST) 21, 18 -- Message-ID: {4548E8AB.4000108-at-tampabay.rr.com} 21, 18 -- Date: Wed, 01 Nov 2006 13:34:19 -0500 21, 18 -- From: Microscopy Today {microscopytoday-at-tampabay.rr.com} 21, 18 -- User-Agent: Thunderbird 1.5.0.7 (Windows/20060909) 21, 18 -- MIME-Version: 1.0 21, 18 -- To: Listserver {Microscopy-at-Microscopy.Com} , 21, 18 -- Confocal Listserver {confocal-at-listserv.buffalo.edu} 21, 18 -- Subject: Microscopy Today November 2006 Table of Contents 21, 18 -- Content-Type: text/plain; charset=windows-1252; format=flowed 21, 18 -- Content-Transfer-Encoding: 8bit 21, 18 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
I have a 1972 Edwards 306 coater, with the 'SEM Planetary Workholder', which is used to carbon-coat samples for EPMA analysis.
It often works OK, and I monitor the coating thickness by watching the color change of a polished brass surface which is in there with the samples. Sometimes to get a satisfactory coating I have to go through two or three cycles of: new carbon rod, evacuate, apply current to the rod until it burns out, bring up to atmospheric pressure, allow the rodholder to cool, which drives me a bit nuts, but it always gets there in the end.
It will coat up to three 47 x 27mm slides or six 1" diameter plugs, or 3 of each, at the same time.
I have two questions:
1 On the very left-hand side of the circuit diagram, the auxiliary contacts of 8-Amp circuit breaker CB2 supply mains power, through a few interlock switches, to a rectangular box labelled 'ILC'. What is this ILC thing? I have pored over the manual, searching for a clue, but have found none. Does anyone know either what ILC is, where it is located, or what current it draws? My problem is that CB2 has started to trip out from time to time, I would like to replace CB2, but I have no idea of the current rating needed for the auxiliary contacts. Also, what is the rectangular box 'AAV' just to the left of the Pirani gauge?
2 I have a window of opportunity to replace the whole unit, but the window will be open for only a short while, and I am not familiar with the current market in vacuum coaters. I would greatly appreciate any specific recommendations (directly to me, if you wish) for suitable replacements. The window is not open very wide, either, so my budget is limited to around US$35K.
thanks in anticipation
Ritchie Sims
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 16, 26 -- From r.sims-at-auckland.ac.nz Wed Nov 1 13:22:49 2006 16, 26 -- Received: from groucho.itss.auckland.ac.nz (groucho.itss.auckland.ac.nz [130.216.190.11]) 16, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA1JMlAC004287 16, 26 -- for {microscopy-at-msa.microscopy.com} ; Wed, 1 Nov 2006 13:22:48 -0600 16, 26 -- Received: from localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) 16, 26 -- by groucho.itss.auckland.ac.nz (Postfix) with ESMTP id 7FB8137933 16, 26 -- for {microscopy-at-msa.microscopy.com} ; Thu, 2 Nov 2006 08:22:45 +1300 (NZDT) 16, 26 -- Received: from groucho.itss.auckland.ac.nz ([127.0.0.1]) 16, 26 -- by localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 16, 26 -- with ESMTP id 04177-19 for {microscopy-at-msa.microscopy.com} ; 16, 26 -- Thu, 2 Nov 2006 08:22:45 +1300 (NZDT) 16, 26 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) 16, 26 -- by groucho.itss.auckland.ac.nz (Postfix) with ESMTP id 80F1937AE3 16, 26 -- for {microscopy-at-msa.microscopy.com} ; Thu, 2 Nov 2006 08:21:07 +1300 (NZDT) 16, 26 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 16, 26 -- To: microscopy-at-msa.microscopy.com 16, 26 -- Date: Thu, 02 Nov 2006 08:21:06 +1300 16, 26 -- MIME-Version: 1.0 16, 26 -- Subject: Carbon Coater questions 16, 26 -- Message-ID: {4549AA72.13398.2AA396-at-localhost} 16, 26 -- Priority: normal 16, 26 -- X-mailer: Pegasus Mail for Windows (4.21c) 16, 26 -- Content-type: text/plain; charset=US-ASCII 16, 26 -- Content-transfer-encoding: 7BIT 16, 26 -- Content-description: Mail message body 16, 26 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
The problem is potentially due to the carbon rods rather than the evaporator. Brown et al. 1991 (Carbon rod failure during carbon coating, The Microscope, v. 39, pp265-267) describe how the quality/composition of the carbon rod can significantly effect the longevity of carbon rods during carbon evaporation.
Cheers, Bryan Bandli
r.sims-at-auckland.ac.nz wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hi } } I have a 1972 Edwards 306 coater, with the 'SEM Planetary } Workholder', which is used to carbon-coat samples for EPMA analysis. } } It often works OK, and I monitor the coating thickness by watching the } color change of a polished brass surface which is in there with the } samples. Sometimes to get a satisfactory coating I have to go through } two or three cycles of: new carbon rod, evacuate, apply current to the } rod until it burns out, bring up to atmospheric pressure, allow the } rodholder to cool, which drives me a bit nuts, but it always gets there in } the end. } } It will coat up to three 47 x 27mm slides or six 1" diameter plugs, or 3 } of each, at the same time. } } I have two questions: } } 1 On the very left-hand side of the circuit diagram, the auxiliary } contacts of 8-Amp circuit breaker CB2 supply mains power, through a } few interlock switches, to a rectangular box labelled 'ILC'. What is this } ILC thing? I have pored over the manual, searching for a clue, but have } found none. Does anyone know either what ILC is, where it is located, } or what current it draws? My problem is that CB2 has started to trip out } from time to time, I would like to replace CB2, but I have no idea of the } current rating needed for the auxiliary contacts. Also, what is the } rectangular box 'AAV' just to the left of the Pirani gauge? } } 2 I have a window of opportunity to replace the whole unit, but the } window will be open for only a short while, and I am not familiar with } the current market in vacuum coaters. I would greatly appreciate any } specific recommendations (directly to me, if you wish) for suitable } replacements. The window is not open very wide, either, so my budget } is limited to around US$35K. } } } thanks in anticipation } } } Ritchie Sims } } } } -- } Ritchie Sims Ph D Phone : 64 9 } 3737599 ext 87713 } Microanalyst Fax : 64 9 3737435 } Department of Geology email : } r.sims-at-auckland.ac.nz } The University of Auckland } Private Bag 92019 } Auckland } New Zealand } } } ==============================Original Headers============================== } 16, 26 -- From r.sims-at-auckland.ac.nz Wed Nov 1 13:22:49 2006 } 16, 26 -- Received: from groucho.itss.auckland.ac.nz (groucho.itss.auckland.ac.nz [130.216.190.11]) } 16, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA1JMlAC004287 } 16, 26 -- for {microscopy-at-msa.microscopy.com} ; Wed, 1 Nov 2006 13:22:48 -0600 } 16, 26 -- Received: from localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) } 16, 26 -- by groucho.itss.auckland.ac.nz (Postfix) with ESMTP id 7FB8137933 } 16, 26 -- for {microscopy-at-msa.microscopy.com} ; Thu, 2 Nov 2006 08:22:45 +1300 (NZDT) } 16, 26 -- Received: from groucho.itss.auckland.ac.nz ([127.0.0.1]) } 16, 26 -- by localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) } 16, 26 -- with ESMTP id 04177-19 for {microscopy-at-msa.microscopy.com} ; } 16, 26 -- Thu, 2 Nov 2006 08:22:45 +1300 (NZDT) } 16, 26 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) } 16, 26 -- by groucho.itss.auckland.ac.nz (Postfix) with ESMTP id 80F1937AE3 } 16, 26 -- for {microscopy-at-msa.microscopy.com} ; Thu, 2 Nov 2006 08:21:07 +1300 (NZDT) } 16, 26 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} } 16, 26 -- To: microscopy-at-msa.microscopy.com } 16, 26 -- Date: Thu, 02 Nov 2006 08:21:06 +1300 } 16, 26 -- MIME-Version: 1.0 } 16, 26 -- Subject: Carbon Coater questions } 16, 26 -- Message-ID: {4549AA72.13398.2AA396-at-localhost} } 16, 26 -- Priority: normal } 16, 26 -- X-mailer: Pegasus Mail for Windows (4.21c) } 16, 26 -- Content-type: text/plain; charset=US-ASCII } 16, 26 -- Content-transfer-encoding: 7BIT } 16, 26 -- Content-description: Mail message body } 16, 26 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz } ==============================End of - Headers============================== } }
-- ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Bryan Bandli Research Microscopist MVA Scientific Consultants 3300 Breckinridge Blvd., Suite 400 (770) 662-8509 ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- The information in this email is confidential and may be legally privileged. It is intended solely for the addressee. If you are not the intended recipient, please delete the email and notify MVA Scientific Consultants of the transmission error. MVA Scientific Consultants - 3300 Breckinridge Blvd. Suite 400, Duluth, GA 30096 - (770)662-8509 -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
==============================Original Headers============================== 7, 20 -- From bbandli-at-mvainc.com Wed Nov 1 13:42:36 2006 7, 20 -- Received: from smtp02.atlngahp.sys.nuvox.net (smtp-out2.atlngahp.sys.nuvox.net [70.43.63.19]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA1JgYjP015107 7, 20 -- for {microscopy-at-msa.microscopy.com} ; Wed, 1 Nov 2006 13:42:35 -0600 7, 20 -- Received: from [192.168.1.95] (216.215.228.34.nw.nuvox.net [216.215.228.34]) 7, 20 -- by smtp02.atlngahp.sys.nuvox.net (8.13.1/8.13.1) with ESMTP id kA1JgOKH016050; 7, 20 -- Wed, 1 Nov 2006 14:42:25 -0500 7, 20 -- Message-ID: {4548F8A6.8040203-at-mvainc.com} 7, 20 -- Date: Wed, 01 Nov 2006 14:42:30 -0500 7, 20 -- From: bbandli {bbandli-at-mvainc.com} 7, 20 -- Reply-To: bbandli-at-mvainc.com 7, 20 -- Organization: MVA Scientific Consultants 7, 20 -- User-Agent: Thunderbird 1.5.0.7 (Windows/20060909) 7, 20 -- MIME-Version: 1.0 7, 20 -- To: r.sims-at-auckland.ac.nz, microscopy-at-msa.microscopy.com 7, 20 -- Subject: Re: [Microscopy] Carbon Coater questions 7, 20 -- References: {200611011923.kA1JNMMa005391-at-ns.microscopy.com} 7, 20 -- In-Reply-To: {200611011923.kA1JNMMa005391-at-ns.microscopy.com} 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cervantes-at-bendres.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] TEM- EDS on vitrified samples
Question: I'm trying to determine whether EDS analysis can be performed successfuly on vitrified cryo TEM samples. I have asked around, and done a few brief literature searches for information on this topic, and haven't got any good answers.
If anyone has any experience in this area, I'd love to hear from you.
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Email: dmcdaniel-at-usuhs.mil Name: Dennis McDaniel
Title-Subject: [Filtered] LR White flat embedding
Question: I have some fibroblasts which were grown on glass coverslips and fixed, dehydrated and infiltrated with LR White. I put a piece of aclar film over the top and polymerized the resin, but now I am having a very difficult time getting the LR White to separate from the coverslip so that I can glue it to an empty BEEM capsule. Does anyone know any good tricks? Thanks.
I have a box here with EM400 analytical pole pieces for +/- 60 degree tilt. At least that's what it says on the box. Is anyone interested in having them? Kim
-- Kim Rensing Ph.D. Manager, Microscopy and Imaging Facility
University of Calgary, Health Sciences Centre B129 - 3330 Hospital Drive NW Calgary, AB, Canada T2N 4N1
403-220-3488 krensing-at-ucalgary.ca
==============================Original Headers============================== 5, 25 -- From krensing-at-ucalgary.ca Thu Nov 2 10:52:48 2006 5, 25 -- Received: from mr1.ucalgary.ca (mr1.ucalgary.ca [136.159.34.165]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA2Gqj3c007053 5, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Nov 2006 10:52:48 -0600 5, 25 -- Received: from smtp2.ucalgary.ca (smtp2.ucalgary.ca [136.159.34.223]) 5, 25 -- by mr1.ucalgary.ca (Postfix) with ESMTP id 8A3157CA8 5, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Nov 2006 09:52:44 -0700 (MST) 5, 25 -- Received: from [192.168.0.100] ([136.159.164.171]) 5, 25 -- (authenticated (0 bits)) 5, 25 -- by smtp2.ucalgary.ca (8.11.7/8.11.6) with ESMTP id kA2GqhQ11327 5, 25 -- (using TLSv1/SSLv3 with cipher DHE-RSA-AES256-SHA (256 bits) verified NO) 5, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Nov 2006 09:52:43 -0700 5, 25 -- Message-ID: {454A2259.4060909-at-ucalgary.ca} 5, 25 -- Date: Thu, 02 Nov 2006 09:52:41 -0700 5, 25 -- From: Kim Rensing {krensing-at-ucalgary.ca} 5, 25 -- Organization: Microscopy and Imaging Facility, U. of Calgary 5, 25 -- User-Agent: Thunderbird 1.5.0.7 (Windows/20060909) 5, 25 -- MIME-Version: 1.0 5, 25 -- To: Microscopy-at-Microscopy.Com 5, 25 -- Subject: EM400 pole pieces 5, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 25 -- Content-Transfer-Encoding: 7bit 5, 25 -- X-UCalgary-MailScanner-Information: Please contact IT Help Desk at (403) 220-5555 for more information 5, 25 -- X-UCalgary-MailScanner: Found to be clean 5, 25 -- X-UCalgary-MailScanner-From: krensing-at-ucalgary.ca ==============================End of - Headers==============================
My samples are indeed particles, which are in an aqueous suspension. The suspensions are vitrified for cryo TEM imaging to observe particles in the pseudo-liquid state. The goal with EDS would be to get elemental mapping of the particles themselves. What I am concerned about is the degradation of the sample upon analysis with EDS, and if enough signal can be detected from particles that may be only 100 nm in diameter.
Our ultimate goal is to characterize differences between the surface of the particle and it's interior, in the solution state. To my limited knowledge, there is not a characterization technique out there that specifically addresses this problem. If there is, I'd be interested in hearing of it.
Thank you, Jessica
-----Original Message----- X-from: Chaoying Ni [mailto:cni-at-UDel.Edu] Sent: Thursday, November 02, 2006 6:12 AM To: Cervantes, Jessica
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Title-Subject: [Filtered] TEM- EDS on vitrified samples
Question: I'm trying to determine whether EDS analysis can be performed successfuly on vitrified cryo TEM samples. I have asked around, and done a few brief literature searches for information on this topic, and haven't got any good answers.
If anyone has any experience in this area, I'd love to hear from you.
Thanks, Jessica Cervantes
==============================Original Headers============================== 21, 14 -- From cervantes-at-bendres.com Thu Nov 2 11:57:34 2006 21, 14 -- Received: from BRIEX04A.bri.local (bc161112.bendcable.com [216.228.161.112]) 21, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA2HvYvp018608 21, 14 -- for {microscopy-at-microscopy.com} ; Thu, 2 Nov 2006 11:57:34 -0600 21, 14 -- MIME-Version: 1.0 21, 14 -- Content-Type: text/plain; 21, 14 -- charset="iso-8859-1" 21, 14 -- Subject: RE: [Microscopy]: TEM- EDS on vitrified samples 21, 14 -- Date: Thu, 2 Nov 2006 09:57:33 -0800 21, 14 -- Message-ID: {8943D65F9AD70E4488AD6DE09F15088768C17F-at-BRIEX04A} 21, 14 -- From: "Cervantes, Jessica" {cervantes-at-bendres.com} 21, 14 -- To: "Chaoying Ni" {cni-at-UDel.Edu} , {microscopy-at-microscopy.com} 21, 14 -- Content-Transfer-Encoding: 8bit 21, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA2HvYvp018608 ==============================End of - Headers==============================
OK. You are right about the possible complication you may run into while you focus a beam onto a particle you want to analyze. I can see the scenario that could happen assuming your particle is non-volatile: amorphous H2O --} crystal H2O + evaporation, left with your particle. I don't see a problem until you have to realize analyzing/mapping the interior and exterior composition of a 100nm solid particle itself sometimes could be a little tricky. If the particle itself is volatile, then good luck and the work should be publishable. -cni
-----Original Message----- X-from: Cervantes, Jessica [mailto:cervantes-at-bendres.com] Sent: Thursday, November 02, 2006 12:58 PM To: Chaoying Ni; microscopy-at-microscopy.com
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Title-Subject: [Filtered] TEM- EDS on vitrified samples
Question: I'm trying to determine whether EDS analysis can be performed successfuly on vitrified cryo TEM samples. I have asked around, and done a few brief literature searches for information on this topic, and haven't got any good answers.
If anyone has any experience in this area, I'd love to hear from you.
Thanks, Jessica Cervantes
==============================Original Headers============================== 25, 26 -- From cni-at-udel.edu Thu Nov 2 12:21:32 2006 25, 26 -- Received: from md3.nss.udel.edu (md3.nss.udel.edu [128.175.1.13]) 25, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA2ILVSE029456 25, 26 -- for {microscopy-at-microscopy.com} ; Thu, 2 Nov 2006 12:21:31 -0600 25, 26 -- Received: from Suba (em076-119.engg.udel.edu [128.175.119.76]) 25, 26 -- by md3.nss.udel.edu (MOS 3.8.2-GA) 25, 26 -- with ESMTP id DLQ55625; 25, 26 -- Thu, 2 Nov 2006 13:21:29 -0500 (EST) 25, 26 -- From: "Chaoying Ni" {cni-at-udel.edu} 25, 26 -- To: "'Cervantes, Jessica'" {cervantes-at-bendres.com} , 25, 26 -- {microscopy-at-microscopy.com} 25, 26 -- Subject: RE: [Microscopy]: TEM- EDS on vitrified samples 25, 26 -- Date: Thu, 2 Nov 2006 13:21:42 -0500 25, 26 -- Organization: University of Delaware 25, 26 -- Message-ID: {000a01c6feab$bf5b6b20$4c77af80-at-Suba} 25, 26 -- MIME-Version: 1.0 25, 26 -- Content-Type: text/plain; 25, 26 -- charset="us-ascii" 25, 26 -- X-Priority: 3 (Normal) 25, 26 -- X-MSMail-Priority: Normal 25, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 25, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 25, 26 -- Importance: Normal 25, 26 -- In-Reply-To: {8943D65F9AD70E4488AD6DE09F15088768C17F-at-BRIEX04A} 25, 26 -- Content-Transfer-Encoding: 8bit 25, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA2ILVSE029456 ==============================End of - Headers==============================
On Nov 1, 2006, at 7:33 PM, cervantes-at-bendres.com wrote:
} Question: I'm trying to determine whether EDS analysis can be } performed successfuly on vitrified cryo TEM samples. I have asked } around, and done a few brief literature searches for information on } this topic, and haven't got any good answers. } } If anyone has any experience in this area, I'd love to hear from you. } Dear Jessica, I did some EDS on the HVEM at Albany on cryo samples, but our set-up was somewhat unusual, so you may have some problems related to the geometry of your instrument. We had our detector mounted so that it looked upward at the specimen at an angle corresponding to a minimum in the brehmsstrahlung distribution, and we tilted the specimen to an angle so that the specimen plane bisected the angle between the beam direction and the detector angle. Our anticontaminator was a blade located between the specimen and the objective aperture--in the HVEM there is a lot of room between the pole pieces. The specimen was a foram with a lot of Si in it, so I got a very good peak and low background. There was essentially no difference between EDS on RT samples and this sample; however, since there is usually a lot of ice and relatively little specimen in a given volume, you may have some S/N problems unless you are looking for an element that is very abundant. Good luck. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Thu Nov 2 13:13:31 2006 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA2JDUBI008547 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Thu, 2 Nov 2006 13:13:31 -0600 4, 22 -- Received: from earth-dog.caltech.edu (earth-dog [192.168.1.3]) 4, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 534702EF1D 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Thu, 2 Nov 2006 11:13:30 -0800 (PST) 4, 22 -- Received: from [192.168.159.233] (jpix-01.caltech.edu [131.215.2.133]) 4, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id 7B3A235490 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Thu, 2 Nov 2006 11:13:24 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v624) 4, 22 -- In-Reply-To: {200611020333.kA23XvIL002787-at-ns.microscopy.com} 4, 22 -- References: {200611020333.kA23XvIL002787-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {b9f598e6c4674c961550c770691cf76b-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] viaWWW: TEM- EDS on vitrified samples 4, 22 -- Date: Thu, 2 Nov 2006 11:18:23 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.624) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.3.3 ==============================End of - Headers==============================
Jessica, not specifically cryo-TEM, but we performed EDS on lyophilized, vitrified (slam freezing) cryo-sections back in '84. Here is the reference:
Harding CV, Susan S, Bobrowski W Elemental profiles in cryosections and frozen-dried bulk specimens of the normal lens. Ophthalmic Res. 1984;16(5):276-83.
If you need it, I can send you a PDF of the publication. Best regards,
Walter F. Bobrowski Sr. Scientist Pfizer Global R&D 2800 Plymouth Rd. Ann Arbor, MI 48105
TEL: 734-622-7814 FAX: 734-622-5718
"The ultimate human freedom is the ability to choose one's attitude in a given set of circumstances." -Viktor Frankl
Title-Subject: [Filtered] TEM- EDS on vitrified samples
Question: I'm trying to determine whether EDS analysis can be performed successfuly on vitrified cryo TEM samples. I have asked around, and done a few brief literature searches for information on this topic, and haven't got any good answers.
If anyone has any experience in this area, I'd love to hear from you.
Thanks, Jessica Cervantes ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately.
==============================Original Headers============================== 15, 29 -- From Walter.Bobrowski-at-pfizer.com Thu Nov 2 13:39:15 2006 15, 29 -- Received: from gromsgoa01.pfizer.com (gromsgo.pfizer.com [148.168.224.84]) 15, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA2JdDpG019378 15, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Nov 2006 13:39:14 -0600 15, 29 -- Received: from groamrexc01.amer.pfizer.com (groamrexc01.amer.pfizer.com [172.30.8.168]) 15, 29 -- by gromsgoa01.pfizer.com (8.13.4/8.13.4) with ESMTP id kA2JcvJ0029861; 15, 29 -- Thu, 2 Nov 2006 14:38:57 -0500 15, 29 -- Received: from groamrexc01.amer.pfizer.com ([172.30.8.157]) by groamrexc01.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.211); 15, 29 -- Thu, 2 Nov 2006 14:39:10 -0500 15, 29 -- Received: from anaamrexm01.amer.pfizer.com ([162.48.101.10]) by groamrexc01.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.211); 15, 29 -- Thu, 2 Nov 2006 14:39:10 -0500 15, 29 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5.7226.0 15, 29 -- Content-class: urn:content-classes:message 15, 29 -- MIME-Version: 1.0 15, 29 -- Content-Type: text/plain; 15, 29 -- charset="US-ASCII" 15, 29 -- Subject: RE: [Microscopy] viaWWW: TEM- EDS on vitrified samples 15, 29 -- Date: Thu, 2 Nov 2006 14:39:10 -0500 15, 29 -- Message-ID: {CF89AF6ABD93B746A55BCCD9480A4B3D07EEC97E-at-anaamrexm01.amer.pfizer.com} 15, 29 -- X-MS-Has-Attach: 15, 29 -- X-MS-TNEF-Correlator: 15, 29 -- Thread-Topic: [Microscopy] viaWWW: TEM- EDS on vitrified samples 15, 29 -- Thread-Index: Acb+MUFW4fTyUaHnQfG07OhNW/5IdQAhJFIw 15, 29 -- From: "Bobrowski, Walter" {Walter.Bobrowski-at-pfizer.com} 15, 29 -- To: {cervantes-at-bendres.com} , {Microscopy-at-microscopy.com} 15, 29 -- X-OriginalArrivalTime: 02 Nov 2006 19:39:10.0711 (UTC) FILETIME=[919CC070:01C6FEB6] 15, 29 -- X-Proofpoint-Spam-Reason: safe 15, 29 -- Content-Transfer-Encoding: 8bit 15, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA2JdDpG019378 ==============================End of - Headers==============================
When I visited Zeiss in Germany, they had a reasonable size unit that polished small to large (metallurgical) samples using plasma. The unit I think sat on a table. It had a slide to the right cover to insert the specimen, then it would slide back and lock. There was a color flat panel LCD display on the far right of the unit to concoct and recall recipes. This was Zeiss' standard way of preparing and cleaning specimens. It was a very nice unit and was reported to be quite reliable and produced excellent results.
Does anyone know what this system might be? I did contact Heiner but so far, no response.
tnx, gary g.
==============================Original Headers============================== 5, 17 -- From gary-at-gaugler.com Thu Nov 2 15:43:17 2006 5, 17 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 5, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kA2LhGi8032617 5, 17 -- for {microscopy-at-microscopy.com} ; Thu, 2 Nov 2006 15:43:16 -0600 5, 17 -- Received: (qmail 17888 invoked from network); 2 Nov 2006 13:43:16 -0800 5, 17 -- Received: by simscan 1.1.0 ppid: 17858, pid: 17883, t: 0.1097s 5, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 5, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 5, 17 -- by qsmtp3 with SMTP; 2 Nov 2006 13:43:16 -0800 5, 17 -- Message-Id: {7.0.1.0.2.20061102133830.024c2d60-at-gaugler.com} 5, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 5, 17 -- Date: Thu, 02 Nov 2006 13:43:09 -0800 5, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 5, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 5, 17 -- Subject: EBSD polishing system 5, 17 -- Mime-Version: 1.0 5, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
} When I visited Zeiss in Germany, they had a reasonable size } unit that polished small to large (metallurgical) samples } using plasma. The unit I think sat on a table. } ... } } Does anyone know what this system might be? I did contact } Heiner but so far, no response.
It may have been something akin to the JEOL SM-09010 "cross section polisher"
Occasionally we encounter situations where, following pretreatments, we achieve successful immunolabeling on resin-embedded thick sectioned samples, but only in distinct areas of the tissue section, where as the rest of the section is unexpectedly unlabelled. What is interesting is that the pattern of (inconsistent) labeling is fairly consistent among all the replicate sections on the glass slide, which randomly dry down in various orientations. At other times, the entire tissue section is labeled successfully.
Has anyone encountered this, and if so, is there an explanation? We're assuming it's a technique issue rather than an issue with either the tissue itself or the resin matrix.
Best regards,
Walter F. Bobrowski Sr. Scientist Pfizer Global R&D 2800 Plymouth Rd. Ann Arbor, MI 48105
TEL: 734-622-7814 FAX: 734-622-5718
"The ultimate human freedom is the ability to choose one's attitude in a given set of circumstances." -Viktor Frankl
---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately.
==============================Original Headers============================== 8, 29 -- From Walter.Bobrowski-at-pfizer.com Fri Nov 3 07:57:47 2006 8, 29 -- Received: from gromsgom01.pfizer.com (gromsgo.pfizer.com [148.168.224.84]) 8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA3DvkvA007191 8, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Nov 2006 07:57:46 -0600 8, 29 -- Received: from groamrexc02.amer.pfizer.com (groamrexc02.amer.pfizer.com [172.30.8.169]) 8, 29 -- by gromsgom01.pfizer.com (8.13.4/8.13.4) with ESMTP id kA3DutjV032233 8, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Nov 2006 08:56:55 -0500 8, 29 -- Received: from mopamrexc02.amer.pfizer.com ([170.116.30.68]) by groamrexc02.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.211); 8, 29 -- Fri, 3 Nov 2006 08:57:45 -0500 8, 29 -- Received: from anaamrexm01.amer.pfizer.com ([162.48.101.10]) by mopamrexc02.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.1830); 8, 29 -- Fri, 3 Nov 2006 08:57:45 -0500 8, 29 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5.7226.0 8, 29 -- Content-class: urn:content-classes:message 8, 29 -- MIME-Version: 1.0 8, 29 -- Content-Type: text/plain; 8, 29 -- charset="US-ASCII" 8, 29 -- Subject: "Spotty" Immunolabeling on Resin-Embedded Samples 8, 29 -- Date: Fri, 3 Nov 2006 08:57:39 -0500 8, 29 -- Message-ID: {CF89AF6ABD93B746A55BCCD9480A4B3D07EECCF4-at-anaamrexm01.amer.pfizer.com} 8, 29 -- X-MS-Has-Attach: 8, 29 -- X-MS-TNEF-Correlator: 8, 29 -- Thread-Topic: "Spotty" Immunolabeling on Resin-Embedded Samples 8, 29 -- Thread-Index: Acb/UAaEstnS5mjmTXeuIO2rRkS3Pw== 8, 29 -- From: "Bobrowski, Walter" {Walter.Bobrowski-at-pfizer.com} 8, 29 -- To: {Microscopy-at-microscopy.com} 8, 29 -- X-OriginalArrivalTime: 03 Nov 2006 13:57:45.0667 (UTC) FILETIME=[09FCD930:01C6FF50] 8, 29 -- X-Proofpoint-Spam-Reason: safe 8, 29 -- Content-Transfer-Encoding: 8bit 8, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA3DvkvA007191 ==============================End of - Headers==============================
I need to quantify total specific staining on immunolabeled microscope slides. I did this many years ago with an old DOS image analysis program (Jandel's JAVA) using the Beer-Lambert equation to compute the total absorbance on a pixel by pixel basis. Does anybody know if there are plug-ins or macros available to do this with ImageJ? I would prefer to use software with a history of use and citation, if possible. Alternatively, is there a better way to quantify staining?
Ralph Common Michigan State University Dept. of Physiology
==============================Original Headers============================== 3, 24 -- From rcommon-at-msu.edu Fri Nov 3 09:13:08 2006 3, 24 -- Received: from sys19.mail.msu.edu (sys19.mail.msu.edu [35.9.75.119]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA3FD8SJ030040 3, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Nov 2006 09:13:08 -0600 3, 24 -- Received: from [35.9.122.125] (helo=emlab) 3, 24 -- by sys19.mail.msu.edu with esmtpsa (Exim 4.52 #1) 3, 24 -- (TLSv1:RC4-MD5:128) 3, 24 -- id 1Gg0jE-0004S4-3o 3, 24 -- for Microscopy-at-microscopy.com; Fri, 03 Nov 2006 10:13:08 -0500 3, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 3, 24 -- To: {Microscopy-at-microscopy.com} 3, 24 -- Subject: Quantifying specific staining 3, 24 -- Date: Fri, 3 Nov 2006 10:14:32 -0500 3, 24 -- Message-ID: {005a01c6ff5a$c4654af0$7d7a0923-at-msu.edu} 3, 24 -- MIME-Version: 1.0 3, 24 -- Content-Type: text/plain; 3, 24 -- charset="iso-8859-1" 3, 24 -- Content-Transfer-Encoding: 7bit 3, 24 -- X-Priority: 3 (Normal) 3, 24 -- X-MSMail-Priority: Normal 3, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 3, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1807 3, 24 -- Importance: Normal 3, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
Its purpose was to polish specimens. Now that I look at the several responses, it must have been an ion mill type of system. It looks similar to the Fishione system in size but the right half of the cover slides to the right to open the chamber. The control console is a flat panel LCD on an articulating arm. As I recall, it used only Ar.
gary g.
At 02:57 AM 11/3/2006, you wrote: } Dear Gary, } } Are you sure it polished as well? I've } seen cleaners (Oxygen plasma to remove surface } grot), but I've never heard of a } polisher/etcher for SEM work. [Although I have } done reactive ion etching of semiconductors using (halogenated) hydrocarbons.] } } Was it anything like the Fishione } instruments 1020? http://www.fischione.com/products/model_1020.asp } } Austin } } P.S. It's very unlikely, but was it was an ion-beam miller? } http://www.gatan.com/specimenprep/691_pips.html } You can do very nice EBSD prep on small areas } using a highly tilted specimen } 80°, low kV } ( {5keV) and a defocused beam. It can also remove surface grot. } } P.P.S. There are other plasma etcher manufacturers, e.g. SPI. } http://www.2spi.com/catalog/instruments/plasma-cleaner.shtml } } ----- Original Message ----- From: {gary-at-gaugler.com} } To: {AuntDaisy-at-gmail.com} } Sent: Thursday, November 02, 2006 9:49 PM } Subject: [Microscopy] EBSD polishing system } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 7, 23 -- From gary-at-gaugler.com Fri Nov 3 10:32:46 2006 7, 23 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kA3GWiTq011063 7, 23 -- for {microscopy-at-microscopy.com} ; Fri, 3 Nov 2006 10:32:45 -0600 7, 23 -- Received: (qmail 3496 invoked from network); 3 Nov 2006 08:32:43 -0800 7, 23 -- Received: by simscan 1.1.0 ppid: 3437, pid: 3494, t: 0.0975s 7, 23 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 23 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 7, 23 -- by qsmtp3 with SMTP; 3 Nov 2006 08:32:43 -0800 7, 23 -- Message-Id: {7.0.1.0.2.20061103082941.02612f20-at-gaugler.com} 7, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 7, 23 -- Date: Fri, 03 Nov 2006 08:32:37 -0800 7, 23 -- To: ADay {auntdaisy-at-gmail.com} 7, 23 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 23 -- Subject: Re: [Microscopy] EBSD polishing system 7, 23 -- Cc: MSA listserver {microscopy-at-microscopy.com} 7, 23 -- In-Reply-To: {005601c6ff36$d08b6130$0100a8c0-at-BigDell} 7, 23 -- References: {200611022149.kA2LnmBJ006384-at-ns.microscopy.com} 7, 23 -- {005601c6ff36$d08b6130$0100a8c0-at-BigDell} 7, 23 -- Mime-Version: 1.0 7, 23 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA3GWiTq011063 ==============================End of - Headers==============================
Gary: I'm wondering if it was an RES100 ion mill by Bal-Tec. I demo'd the RES100 back when it first came out and if my memory is reliable (sometimes not) it had a sliding cover. I don't think they sell the RES100 anymore. I recommend the RES101, the latest generation of that tool, which I have and use almost everyday. I use it for SEM sample clean-up and cross-section delineation. In the USA, Bal-Tec is rep'd by RMC Boeckler. http://www.baltec-rmc.com/cms/index.cfm/path/17933/26002/24159/25864/ PS: there are NO spaces in the URL and I have no financial interest in the company.
gary-at-gaugler.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Its purpose was to polish specimens. Now that I look } at the several responses, it must have been an ion mill } type of system. It looks similar to the Fishione system } in size but the right half of the cover slides to the right } to open the chamber. The control console is a flat panel } LCD on an articulating arm. As I recall, it used only } Ar. } } gary g. } } } } At 02:57 AM 11/3/2006, you wrote: } } } Dear Gary, } } } } Are you sure it polished as well? I've } } seen cleaners (Oxygen plasma to remove surface } } grot), but I've never heard of a } } polisher/etcher for SEM work. [Although I have } } done reactive ion etching of semiconductors using (halogenated) hydrocarbons.] } } } } Was it anything like the Fishione } } instruments 1020? http://www.fischione.com/products/model_1020.asp } } } } Austin } } } } P.S. It's very unlikely, but was it was an ion-beam miller? } } http://www.gatan.com/specimenprep/691_pips.html } } You can do very nice EBSD prep on small areas } } using a highly tilted specimen } 80°, low kV } } ( {5keV) and a defocused beam. It can also remove surface grot. } } } } P.P.S. There are other plasma etcher manufacturers, e.g. SPI. } } http://www.2spi.com/catalog/instruments/plasma-cleaner.shtml } } } } ----- Original Message ----- From: {gary-at-gaugler.com} } } To: {AuntDaisy-at-gmail.com} } } Sent: Thursday, November 02, 2006 9:49 PM } } Subject: [Microscopy] EBSD polishing system } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Hi Listers: } } } } } } When I visited Zeiss in Germany, they had a reasonable } } } size unit that polished small to large (metallurgical) } } } samples using plasma. The unit I think sat on a table. } } } It had a slide to the right cover to insert the specimen, } } } then it would slide back and lock. There was a color } } } flat panel LCD display on the far right of the unit to } } } concoct and recall recipes. This was Zeiss' standard } } } way of preparing and cleaning specimens. It was a very } } } nice unit and was reported to be quite reliable and produced } } } excellent results. } } } } } } Does anyone know what this system might be? I did } } } contact Heiner but so far, no response. } } } } } } tnx, } } } gary g. } } } } } } } } } ==============================Original Headers============================== } } } 5, 17 -- From gary-at-gaugler.com Thu Nov 2 15:43:17 2006 } } } 5, 17 -- Received: from qsmtp3.mc.surewest.net } } } (qsmtp.mc.surewest.net [66.60.130.145]) } } } 5, 17 -- by ns.microscopy.com } } } (8.12.11.20060308/8.12.8) with SMTP id kA2LhGi8032617 } } } 5, 17 -- for {microscopy-at-microscopy.com} ; Thu, 2 Nov 2006 15:43:16 -0600 } } } 5, 17 -- Received: (qmail 17888 invoked from } } } network); 2 Nov 2006 13:43:16 -0800 } } } 5, 17 -- Received: by simscan 1.1.0 ppid: 17858, pid: 17883, t: 0.1097s } } } 5, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 } } } 5, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) } } } 5, 17 -- by qsmtp3 with SMTP; 2 Nov 2006 13:43:16 -0800 } } } 5, 17 -- Message-Id: {7.0.1.0.2.20061102133830.024c2d60-at-gaugler.com} } } } 5, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 } } } 5, 17 -- Date: Thu, 02 Nov 2006 13:43:09 -0800 } } } 5, 17 -- To: MSA listserver {microscopy-at-microscopy.com} } } } 5, 17 -- From: Gary Gaugler {gary-at-gaugler.com} } } } 5, 17 -- Subject: EBSD polishing system } } } 5, 17 -- Mime-Version: 1.0 } } } 5, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } } } ==============================End of - Headers============================== } } } } } } } ==============================Original Headers============================== } 7, 23 -- From gary-at-gaugler.com Fri Nov 3 10:32:46 2006 } 7, 23 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) } 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kA3GWiTq011063 } 7, 23 -- for {microscopy-at-microscopy.com} ; Fri, 3 Nov 2006 10:32:45 -0600 } 7, 23 -- Received: (qmail 3496 invoked from network); 3 Nov 2006 08:32:43 -0800 } 7, 23 -- Received: by simscan 1.1.0 ppid: 3437, pid: 3494, t: 0.0975s } 7, 23 -- scanners: regex: 1.1.0 attach: 1.1.0 } 7, 23 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) } 7, 23 -- by qsmtp3 with SMTP; 3 Nov 2006 08:32:43 -0800 } 7, 23 -- Message-Id: {7.0.1.0.2.20061103082941.02612f20-at-gaugler.com} } 7, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 } 7, 23 -- Date: Fri, 03 Nov 2006 08:32:37 -0800 } 7, 23 -- To: ADay {auntdaisy-at-gmail.com} } 7, 23 -- From: Gary Gaugler {gary-at-gaugler.com} } 7, 23 -- Subject: Re: [Microscopy] EBSD polishing system } 7, 23 -- Cc: MSA listserver {microscopy-at-microscopy.com} } 7, 23 -- In-Reply-To: {005601c6ff36$d08b6130$0100a8c0-at-BigDell} } 7, 23 -- References: {200611022149.kA2LnmBJ006384-at-ns.microscopy.com} } 7, 23 -- {005601c6ff36$d08b6130$0100a8c0-at-BigDell} } 7, 23 -- Mime-Version: 1.0 } 7, 23 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed } 7, 23 -- Content-Transfer-Encoding: 8bit } 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA3GWiTq011063 } ==============================End of - Headers============================== } }
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Email: dgmorgan-at-ucdavis.edu Name: David Morgan
Organization: UC Davis
Title-Subject: [Filtered] Tantalum foils
Question: Hi,
I find myself in need of a tantalum foil suitable for TEM and since I am just getting into the field of materials science, I am hoping that this sort of sample is commercially available. Are such things available and can anyone suggest a vendor? Thanks in advance.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both elchem33-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: elchem33-at-gmail.com Name: Marco Puzi
Organization: Dep of Chemistry and Biochemistry, Univ Split, Croatia
Title-Subject: [Filtered] TEM alignment problem
Question: Hi!
During the alignment of our Jeol JEM-200CX TEM something has gone wrong. I suppose that user by mistake used the gun/beam tilt controls instead the shift (trans) controls, or he altered the sequence for gun and condenser alignment; after that, it seems that he used other controls (deflector coils X and Y corrector/compensator, beam displacement compensating coil... I even suspect that he mechanically moved the intermediate and projector lens) in attempt to align the microscope. As a result, the microscope is terribly misaligned and I can't seem to get it back in the aligned state following the alignment procedure given in Jeol manual. After the gun and condenser alignment steps everything seems fine (beam is centered on the screen and expands uniformly). However, when cond alignment wobbler is turned on, the beam separates into two spots, and one spot is blurred to aprox. 1 cm in diameter, while other remains focused! Also, when specimen is placed in microscope, image moves badly during focusing and becomes blurred during the change of illumination. I suppose that all this effects are due to the tilted beam.
Does anybody know the alignment procedure that works in similar situations?
Also, I will be grateful if anyone could explain me what beam displacement compensating coils (located between condenser and objective lens, just below beam deflector coils) exactly do (this topic is not covered in manual).
You can buy sheets of Tantalum metal at Goodfellow They sell small quantities with high purity for research.
Here is the link.
http://www.goodfellow.com/
Just search for tantalum foils.
These are generally suitable for you to electropolish to make TEM samples. Warning I believe anumber of the polishing solutions tend to use HF, nasty stuff. If your new to TEM sample method make sure you know the hazards of HF.
Nestor Your Friendly Neighborhood SysOp
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==============================Original Headers============================== 15, 13 -- From zaluzec-at-microscopy.com Sat Nov 4 12:44:14 2006 15, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 15, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA4IiEN1014959; 15, 13 -- Sat, 4 Nov 2006 12:44:14 -0600 15, 13 -- Mime-Version: 1.0 15, 13 -- Message-Id: {p06110402c1728d62bb1d-at-[206.69.208.22]} 15, 13 -- In-Reply-To: {200611041829.kA4ITo4u026249-at-ns.microscopy.com} 15, 13 -- References: {200611041829.kA4ITo4u026249-at-ns.microscopy.com} 15, 13 -- Date: Sat, 4 Nov 2006 12:44:14 -0600 15, 13 -- To: dgmorgan-at-ucdavis.edu, microscopy-at-microscopy.com 15, 13 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 15, 13 -- Subject: Re: [Microscopy] viaWWW: Tantalum foils 15, 13 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
D. Morgan asked: ============================================= I find myself in need of a tantalum foil suitable for TEM and since I am just getting into the field of materials science, I am hoping that this sort of sample is commercially available. Are such things available and can anyone suggest a vendor? Thanks in advance. ============================================= If you are talking about a tantalum foil on a TEM grid, see URL http://www.2spi.com/catalog/standards/aem.shtml
This is a standard foil product produced by SPI Supplies.
Disclaimer: SPI Supplies is the manufacturer of this foil sample.
Chuck =================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
==============================Original Headers============================== 11, 30 -- From cgarber-at-2spi.com Sat Nov 4 14:56:41 2006 11, 30 -- Received: from s-utl01-atpop.stsn.net (s-utl01-atpop.stsn.net [72.254.128.201]) 11, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kA4KueDT003474 11, 30 -- for {microscopy-at-msa.microscopy.com} ; Sat, 4 Nov 2006 14:56:40 -0600 11, 30 -- Received: from s-utl01-atpop.stsn.net ([127.0.0.1]) 11, 30 -- by s-utl01-atpop.stsn.net (SMSSMTP 4.1.2.20) with SMTP id M2006110415563923259 11, 30 -- for {microscopy-at-msa.microscopy.com} ; Sat, 04 Nov 2006 15:56:39 -0500 11, 30 -- X-Spam-Status: No, hits=0.0 required=9.9 11, 30 -- tests=ALL_TRUSTED: -2.867,AWL: 0.139,BAYES_00: -1.665, 11, 30 -- SARE_RECV_ADDR: 0.027 11, 30 -- X-Spam-Level: 11, 30 -- Received: from ibm1x23g2abfyg ([10.1.154.1]) 11, 30 -- by s-utl01-atpop.stsn.net 11, 30 -- for microscopy-at-msa.microscopy.com; 11, 30 -- Sat, 4 Nov 2006 15:56:38 -0500 11, 30 -- Message-ID: {001d01c70053$8daa43e0$019a010a-at-ibm1x23g2abfyg} 11, 30 -- Reply-To: "Garber, Charles A." {cgarber-at-2spi.com} 11, 30 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 11, 30 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 11, 30 -- Subject: Tantalum foil on a grid 11, 30 -- Date: Sat, 4 Nov 2006 15:55:15 -0500 11, 30 -- MIME-Version: 1.0 11, 30 -- Content-Type: text/plain; 11, 30 -- charset="Windows-1252" 11, 30 -- X-Priority: 3 11, 30 -- X-MSMail-Priority: Normal 11, 30 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2869 11, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 11, 30 -- Content-Transfer-Encoding: 8bit 11, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA4KueDT003474 ==============================End of - Headers==============================
Also available from other electron microscope supply companies. You might want to do a price check.
Ted Dunn The EMscope Company Ltd. Thailand
D. Morgan asked: ============================================= I find myself in need of a tantalum foil suitable for TEM
--- cgarber-at-2spi.com wrote:
============================================= } If you are talking about a tantalum foil on a TEM } grid, see URL } http://www.2spi.com/catalog/standards/aem.shtml } } This is a standard foil product produced by SPI } Supplies. } } Disclaimer: SPI Supplies is the manufacturer of } this foil sample. } } Chuck } =================================================== } Charles A. Garber, Ph. D. Ph: } 1-(610)-436-5400 } President } SPI SUPPLIES FAX: } 1-(610)-436-5755 } PO BOX 656 } e-mail: cgarber-at-2spi.com } West Chester, PA 19381-0656 USA Cust. Service: } spi2spi-at-2spi.com } } } Look for us! } ############################ } WWW: http://www.2spi.com } ############################ } ================================================== } } } } } } } ==============================Original } Headers============================== } 11, 30 -- From cgarber-at-2spi.com Sat Nov 4 14:56:41 } 2006 } 11, 30 -- Received: from s-utl01-atpop.stsn.net } (s-utl01-atpop.stsn.net [72.254.128.201]) } 11, 30 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with SMTP id } kA4KueDT003474 } 11, 30 -- for {microscopy-at-msa.microscopy.com} ; Sat, } 4 Nov 2006 14:56:40 -0600 } 11, 30 -- Received: from s-utl01-atpop.stsn.net } ([127.0.0.1]) } 11, 30 -- by s-utl01-atpop.stsn.net (SMSSMTP } 4.1.2.20) with SMTP id M2006110415563923259 } 11, 30 -- for {microscopy-at-msa.microscopy.com} ; Sat, } 04 Nov 2006 15:56:39 -0500 } 11, 30 -- X-Spam-Status: No, hits=0.0 required=9.9 } 11, 30 -- tests=ALL_TRUSTED: -2.867,AWL: } 0.139,BAYES_00: -1.665, } 11, 30 -- SARE_RECV_ADDR: 0.027 } 11, 30 -- X-Spam-Level: } 11, 30 -- Received: from ibm1x23g2abfyg } ([10.1.154.1]) } 11, 30 -- by s-utl01-atpop.stsn.net } 11, 30 -- for microscopy-at-msa.microscopy.com; } 11, 30 -- Sat, 4 Nov 2006 15:56:38 -0500 } 11, 30 -- Message-ID: } {001d01c70053$8daa43e0$019a010a-at-ibm1x23g2abfyg} } 11, 30 -- Reply-To: "Garber, Charles A." } {cgarber-at-2spi.com} } 11, 30 -- From: "Garber, Charles A." } {cgarber-at-2spi.com} } 11, 30 -- To: "Microscopy Listserver" } {microscopy-at-msa.microscopy.com} } 11, 30 -- Subject: Tantalum foil on a grid } 11, 30 -- Date: Sat, 4 Nov 2006 15:55:15 -0500 } 11, 30 -- MIME-Version: 1.0 } 11, 30 -- Content-Type: text/plain; } 11, 30 -- charset="Windows-1252" } 11, 30 -- X-Priority: 3 } 11, 30 -- X-MSMail-Priority: Normal } 11, 30 -- X-Mailer: Microsoft Outlook Express } 6.00.2900.2869 } 11, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE } V6.00.2900.2962 } 11, 30 -- Content-Transfer-Encoding: 8bit } 11, 30 -- X-MIME-Autoconverted: from } quoted-printable to 8bit by ns.microscopy.com id } kA4KueDT003474 } ==============================End of - } Headers============================== }
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==============================Original Headers============================== 10, 19 -- From drteddunne-at-yahoo.com Sat Nov 4 23:37:29 2006 10, 19 -- Received: from web33413.mail.mud.yahoo.com (web33413.mail.mud.yahoo.com [68.142.206.145]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kA55bTi5003526 10, 19 -- for {microscopy-at-microscopy.com} ; Sat, 4 Nov 2006 23:37:29 -0600 10, 19 -- Received: (qmail 75617 invoked by uid 60001); 5 Nov 2006 05:37:28 -0000 10, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 10, 19 -- s=s1024; d=yahoo.com; 10, 19 -- h=Message-ID:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 10, 19 -- b=yF+sNbUCO3kL7voiLV9qllMzj/R1qXJ8jPKtZGl+Y2lJdVeore8q+rD7mdjLjZIyeJnzqUxlPdmh4pqnRfZtQImH8hSQqePk1+aOWUfaem+JYntpIfzKtQpEG3ggsE7VoTinI09FLKnZby9QLTcjmcikwVVAOtqfndKzTzZFPxw= ; 10, 19 -- Message-ID: {20061105053728.75615.qmail-at-web33413.mail.mud.yahoo.com} 10, 19 -- Received: from [203.190.250.105] by web33413.mail.mud.yahoo.com via HTTP; Sat, 04 Nov 2006 21:37:28 PST 10, 19 -- Date: Sat, 4 Nov 2006 21:37:28 -0800 (PST) 10, 19 -- From: ted dunn {drteddunne-at-yahoo.com} 10, 19 -- Subject: Re: [Microscopy] Tantalum foil on a grid 10, 19 -- To: microscopy-at-microscopy.com 10, 19 -- In-Reply-To: {200611042059.kA4Kxi21008928-at-ns.microscopy.com} 10, 19 -- MIME-Version: 1.0 10, 19 -- Content-Type: text/plain; charset=iso-8859-1 10, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
However, things may not be as bad as you think. You say that the user may have adjusted the IL and PL mechanical alignments, that is possible but in over 35 years of looking after EMs in government and university labs I've never met a user who adjusted the mechanical alignment. You will soon tell if they have because the Mag images and Diff patterns will not be centered when you go down in mag or camera length. Yes - go down not up. If the image is not quite centered at 1K it will be a long way out at 100K but if it's a bit out at 100K it will be centered at 1K.
So to your Beam Compensation controls. You have two sets of 4 four coils one below the other, each set has 2 X coils and 2 Y coils with X and Y set at 90deg to each other. These are often called Beam Shift (top set) and Beam Tilt (lower set). However both sets are used to give shift and tilt. Imagine you have a beam running down the column and you pass a current through the top X set, this will deflect the beam through an angle - alpha. If you then pass an equal current in the opposite direction through the X coils in the lower set the beam will be deflected through an angle, alpha, but in the opposite direction. The beam will now be parallel with the original direction but moved (shifted) a little. Now apply a higher current through the lower set and the beam will be deflected through a greater angle. If higher current is set correctly the beam will pass through the same point in the specimen as the undefleted beam would have but now it is at an angle (beta) to the original beam. This is pure tilt, increase the current through both the upper and lower sets in the same ratio and the angle (beta) changes. The beam shift control adjusts the coils in the correct ratio to shift and the beam tilt control adjusts them in the correct ratio to tilt. This is a whole lot easier to understand if you draw it out.
With the beam compensators you are setting up pure tilt onto the specimen. The microscope wobbles the beam and you adjust the ratio until both spots coincide. Under this condition when you tilt the beam (eg. for dark field imaging) it does not move off the area of interest.
Now what about your problem - I think that the distortion you see is caused by the beam being deflected too far from the optic axis. First (assuming you have a side entry 200CX) make sure the specimen is at eucentric height (image does not move when you tilt the specimen rod) then focus the specimen. If you have a top entry 200CX then set the objective current to the correct value. Next set the beam tilts to their mid positions, that is when both indicator arrows are lit or when it changes from one to the other. If the beam tilt is a long way out the image will move a lot as you adjust the objective lens current.
Now try the beam compensation again. I suspect that either the objective lens current is wrong or the beam tilt is a long way out to begin with.
Of course it is always possible that the user has adjusted the mechanical alignments or that lenses, deflectors or HT are not working properly but in my experience that is less likely.
I hope this helps.
Good luck, Ron
In message {200611041839.kA4IdG3M012999-at-ns.microscopy.com} elchem33-at-gmail.com writes: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both elchem33-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: elchem33-at-gmail.com } Name: Marco Puzi } } Organization: Dep of Chemistry and Biochemistry, Univ Split, Croatia } } Title-Subject: [Filtered] TEM alignment problem } } Question: Hi! } } During the alignment of our Jeol JEM-200CX TEM something has gone wrong. I suppose that user by mistake used the gun/beam tilt controls instead the shift (trans) controls, or he altered the sequence for gun and condenser alignment; after that, it seems that he used other controls (deflector coils X and Y corrector/compensator, beam displacement compensating coil... I even suspect that he mechanically moved the intermediate and projector lens) in attempt to align the microscope. As a result, the microscope is terribly misaligned and I can't seem to get it back in the aligned state following the alignment procedure given in Jeol manual. After the gun and condenser alignment steps everything seems fine (beam is centered on the screen and expands uniformly). However, when cond alignment wobbler is turned on, the beam separates into two spots, and one spot is blurred to aprox. 1 cm in diameter, while other remains focused! Also, when specimen is placed in microscope, image moves ! } badly during focusing and becomes blurred during the change of illumination. I suppose that all this effects are due to the tilted beam. } } Does anybody know the alignment procedure that works in similar situations? } } Also, I will be grateful if anyone could explain me what beam displacement compensating coils (located between condenser and objective lens, just below beam deflector coils) exactly do (this topic is not covered in manual). } } Thanx in advance. } Marco } } } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 12, 14 -- From zaluzec-at-microscopy.com Sat Nov 4 12:31:07 2006 } 12, 14 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 12, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA4IV6TA027303 } 12, 14 -- for {microscopy-at-microscopy.com} ; Sat, 4 Nov 2006 12:31:07 -0600 } 12, 14 -- Mime-Version: 1.0 } 12, 14 -- X-Sender: (Unverified) } 12, 14 -- Message-Id: {p06110401c1728ca58ed7-at-[206.69.208.22]} } 12, 14 -- Date: Sat, 4 Nov 2006 12:31:07 -0600 } 12, 14 -- To: microscopy-at-microscopy.com } 12, 14 -- From: elchem33-at-gmail.com (by way of MicroscopyListserver) } 12, 14 -- Subject: viaWWW: TEM alignment problem Help needed } 12, 14 -- Content-Type: text/plain; charset="us-ascii" } 12, 14 -- Content-Transfer-Encoding: 8bit } 12, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA4IV6TA027303 } ==============================End of - Headers============================== }
-- Mr. Ron Doole Department of Materials Senior Instrumentation Engineer. University of Oxford. Phone +44 (0) 1865 273701 Parks Road. Oxford. OX1 3PH Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
==============================Original Headers============================== 14, 24 -- From ron.doole-at-materials.ox.ac.uk Sun Nov 5 15:48:48 2006 14, 24 -- Received: from relay0.mail.ox.ac.uk (relay0.mail.ox.ac.uk [129.67.1.161]) 14, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA5Lml3j013170 14, 24 -- for {microscopy-at-msa.microscopy.com} ; Sun, 5 Nov 2006 15:48:47 -0600 14, 24 -- Received: from webmail218.herald.ox.ac.uk ([163.1.0.218]) 14, 24 -- by relay0.mail.ox.ac.uk with esmtp (Exim 4.62) 14, 24 -- (envelope-from {ron.doole-at-materials.ox.ac.uk} ) 14, 24 -- id 1GgprC-0007IN-37; Sun, 05 Nov 2006 21:48:47 +0000 14, 24 -- Received: by webmail218.herald.ox.ac.uk (Postfix, from userid 33) 14, 24 -- id E75D65A078; Sun, 5 Nov 2006 21:48:46 +0000 (GMT) 14, 24 -- Content-Type: text/plain 14, 24 -- Content-Disposition: inline 14, 24 -- Content-Transfer-Encoding: 7bit 14, 24 -- MIME-Version: 1.0 14, 24 -- X-Mailer: MIME-tools 5.417 (Entity 5.417) 14, 24 -- From: Ron Doole {ron.doole-at-materials.ox.ac.uk} 14, 24 -- Date: Sun, 05 Nov 2006 21:48:46 +0000 14, 24 -- To: elchem33-at-gmail.com 14, 24 -- Cc: microscopy-at-msa.microscopy.com 14, 24 -- Subject: Re: [Microscopy] viaWWW: TEM alignment problem Help needed 14, 24 -- In-Reply-To: {200611041839.kA4IdG3M012999-at-ns.microscopy.com} 14, 24 -- X-Webmail-Sender: rdoole 14, 24 -- X-Webmail-Originating-Ip: 86.134.92.244 14, 24 -- Message-Id: {20061105214846.E75D65A078-at-webmail218.herald.ox.ac.uk} ==============================End of - Headers==============================
We shall be having an Open Day for students next week and one of the highlights this time is 'showcasing' our newly acquired SEM unit. Any ideas on how I could present the new toy to them and catch their attention? Posters or movies you could share perhaps? =)
Our unit is a FEI Quanta 200, by the way.
Thanks and best regards,
Melina L. Miralles PGSc Laboratory Technician The Petroleum Institute Tel: 02-5085497 (Office) Tel: 02-5085539/5481 (Lab) Fax : 02-5085423
==============================Original Headers============================== 9, 27 -- From mmiralles-at-pi.ac.ae Mon Nov 6 01:26:42 2006 9, 27 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA67QdON002122 9, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Nov 2006 01:26:41 -0600 9, 27 -- Received: from pi-exf.pi.ac.ae ([192.168.2.11]) 9, 27 -- by mx1.pi.ac.ae with ESMTP; 06 Nov 2006 11:29:33 +0400 9, 27 -- X-IronPort-AV: i="4.09,390,1157313600"; 9, 27 -- d="scan'208"; a="829514:sNHT21947008" 9, 27 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.1830); 9, 27 -- Mon, 6 Nov 2006 11:26:23 +0400 9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 27 -- Content-class: urn:content-classes:message 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Type: text/plain; 9, 27 -- charset="us-ascii" 9, 27 -- Subject: Open Day Ideas?? 9, 27 -- Date: Mon, 6 Nov 2006 11:26:23 +0400 9, 27 -- Message-ID: {D5603421C6303A46883F87E7968F285B01F69E28-at-pi-exm.PI.AC.AE} 9, 27 -- X-MS-Has-Attach: 9, 27 -- X-MS-TNEF-Correlator: 9, 27 -- Thread-Topic: Open Day Ideas?? 9, 27 -- Thread-Index: AccBdN0FLsnEqkD4SHGdRuMNTtUBeQ== 9, 27 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae} 9, 27 -- To: {Microscopy-at-microscopy.com} 9, 27 -- X-OriginalArrivalTime: 06 Nov 2006 07:26:23.0950 (UTC) FILETIME=[DD086EE0:01C70174] 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA67QdON002122 ==============================End of - Headers==============================
Hello all, we have following trouble: "Panasonic Lithium battery BR-2/3A 3V" which are used for battery back up in Philips CM12 electron microscope were exhausted after one year of use. This occurred for the second time. Is this normal ? Previously we had to replace them after several years but now we have to use the third set of batteries in two years. Has anybody any suggestion or hint? Thanking you in advance. Oldrich
----------------------------------------------- Oldrich Benada Institute of Microbiology, Acad. Sci. CR Videnska 1083 142 20 Prague 4 Czech Republic
==============================Original Headers============================== 5, 20 -- From benada-at-biomed.cas.cz Mon Nov 6 04:14:43 2006 5, 20 -- Received: from biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA6AEglN016074 5, 20 -- for {microscopy-at-microscopy.com} ; Mon, 6 Nov 2006 04:14:43 -0600 5, 20 -- Received: from [147.231.44.101] (u117ob.mbu.cas.cz [147.231.44.101]) 5, 20 -- by biomed.cas.cz (8.13.3+Sun/8.13.3) with ESMTP id kA6AEKDt010340 5, 20 -- for {microscopy-at-microscopy.com} ; Mon, 6 Nov 2006 11:14:20 +0100 (CET) 5, 20 -- From: benada-at-biomed.cas.cz 5, 20 -- To: microscopy-at-microscopy.com 5, 20 -- Date: Mon, 06 Nov 2006 11:14:39 +0100 5, 20 -- MIME-Version: 1.0 5, 20 -- Subject: Battery back up trouble 5, 20 -- Message-ID: {454F191F.4142.2BF28C-at-benada.biomed.cas.cz} 5, 20 -- Priority: normal 5, 20 -- X-mailer: Pegasus Mail for Windows (4.41) 5, 20 -- Content-type: text/plain; charset=US-ASCII 5, 20 -- Content-transfer-encoding: 7BIT 5, 20 -- Content-description: Mail message body 5, 20 -- X-Antivirus: avast! (VPS 0645-4, 03.11.2006), Outbound message 5, 20 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
I used a 1978 vintage Edwards 306A evaporator. I found a local microscopist with some 1980s E306A documentation and got some copies made. I have repaired or reconditioned at least three different evaporators over the years including an E306.
I believe CB1 and CB2 are 8 amp ganged double pole thermal circuit breakers that are manually reset and latched closed. This ganging on CB2 is set up so that tripping either CB2-8A -OR- CB2-I/LOCK will cut off power through the two ILC switch contacts (shown in diagrams) and that are controlled (not shown) by the ILC "box". The ITC box is a contactor relay coil. The only normal way to trip the CB2-8A half is to have a current over 8 amps. For example, the RP motor stalls out and the current rises. Breaking circuit continuity near CB2-8A or shorting across that half of CB2 will not trip CB2-I/LOCK. CB2-I/LOCK holds an electrical latch on the ILC "box" contactor coil and that keeps the power turned on to the E306 through both sets of contacts labeled ITC. Since that "relay" coil controls the ITC contacts, CB2-I/LOCK is a latched power supply safety interlock circuit switch. The shunt jumper above the ITC box is shown as an unlabeled wire between terminals with wires 2 and 8 on them in the terminal block diagram. The shunt is not shown as an interlock switch. The analysis of CB2-I/LOCK failure was sent separately. Basically any open in the ITC box branch circuit between wires 1 and 4 shuts off power. Any failure in the RP, DP, or Pirani section that causes a higher current draw through CB2-8A will shut down the power circuits. CB2-8A could be damaged and tripping out at a lower current, however. Monitor the current with a clamp-on ammeter to see how close to 8 amps it is running.
You asked about terms. These may be of general interest to E306A owners. AAV-Air Admit Valve HT acc- High Tension Accessory or voltage supply. (Shown as a dashed line rectangular box near the step down transformer, is located between wires 30 & 14, and not labeled as HT in some diagrams.) LTC- Low Tension Contactor. CB2 I/LOCK- InterLock branch of ganged Circuit Breaker two ILC box- InterLock Contactor unit. This is a double pole 230 VAC line voltage detection relay and is latched ON by pushing the CB2 reset. Circle X's- Neon indicator lights.
You said, "the auxiliary contacts of 8-Amp circuit breaker CB2 supply mains power, through a few interlock switches, to a rectangular box labeled 'ILC'." This might mean, "the auxiliary contacts (CB2-I/LOCK) of the dual 8-Amp circuit breaker CB2, supply mains power through an interlock switch (shunt jumper or link above the ILC box) to a rectangular box labeled 'ILC'. What is this ITC thing?" It's a contactor relay coil.
The ILC box should have a dashed line that goes from it to the dashed line that connects the two ganged ILC switch contacts in the main 230 VAC power line feeders. This ILC dashed line would connect near the words MAINS ON which is really a label for the MAINS neon indicator light on the top user's panel.
CB1-8A supplies power through "a few interlock switches", some door interlock switches and to a rectangular box labeled 'LTC' and another shunt. So what is this LTC thing? It is a contactor or coil that operates the LTC switch contacts at the far right, IMO. You turn on LT and those contacts close. That's another missing dashed line to signify remote control of relay contacts.
You mentioned carbon rod evaporations for a reason. I and others had problems on the 306A Edwards with the carbon rod holder too. Check all the connections and clean them, if that is also a problem. Clean the inside surfaces where the rods go. Most of these older stand alone evaporators are bulletproof and still valuable. I restored a second evaporator unit and converted it to the ball bearing operated LADD dual carbon rod and tungsten basket system. Earlier this year I was studying the theory of side emissions of electrons from heated tungsten wires and nichrome wires reputed to cause the false peak. When the wires failed to continue heating, it was always the tightness of the connections at the W wires that were the problem. I don't like to torque things too tight. My prolonged heating and "looser" mechanical connection caused contact failures at the set screws during this prolonged heating. It was not the fault of the LADD unit or my home-made tungsten hairpin holder. (I hate fishing out a slightly bent, jammed and broken W wire from a tiny hole in a wire holder in my garage.) I suspect the same type of contact connection problems for the Edwards carbon rod clamping collars. We really had to torque them very tight.
The rating on CB2-I/LOCK should not be that important but is probably 8 amps at 230 VAC or higher. The ratings should be printed on the items CB2, CB1, and the ILC contactor itself. I am not sure if CB2 is a slow blow circuit breaker or not. I'd call Edwards for a new CB2 unit.
HTH,
Paul
At 01:23 PM 11/1/06 -0600, you wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 11, 25 -- From beaurega-at-westol.com Mon Nov 6 08:54:56 2006 11, 25 -- Received: from smtp-gateway-2.winbeam.com (smtp-gateway-2.winbeam.com [64.84.97.67]) 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA6EstEG031824 11, 25 -- for {microscopy-at-msa.microscopy.com} ; Mon, 6 Nov 2006 08:54:55 -0600 11, 25 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 11, 25 -- by smtp-gateway-2.winbeam.com (8.13.1/8.12.8) with SMTP id kA6EruJ1011062 11, 25 -- for {microscopy-at-msa.microscopy.com} ; Mon, 6 Nov 2006 09:53:56 -0500 11, 25 -- Received: (qmail 17504 invoked by uid 89); 6 Nov 2006 14:53:53 -0000 11, 25 -- Received: from pitts-69-72-22-30.dynamic-dialup.coretel.net (HELO millenium) (69.72.22.30) 11, 25 -- by mail.winbeam.com with SMTP; 6 Nov 2006 14:53:53 -0000 11, 25 -- Message-Id: {3.0.6.32.20061106095416.007da390-at-pop3.norton.antivirus} 11, 25 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus (Unverified) 11, 25 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 11, 25 -- Date: Mon, 06 Nov 2006 09:54:16 -0500 11, 25 -- To: r.sims-at-auckland.ac.nz, microscopy-at-msa.microscopy.com 11, 25 -- From: Beaurega {beaurega-at-westol.com} 11, 25 -- Subject: Re: [Microscopy] Carbon Coater questions 11, 25 -- In-Reply-To: {200611011923.kA1JNFik005070-at-ns.microscopy.com} 11, 25 -- Mime-Version: 1.0 11, 25 -- Content-Type: text/plain; charset="us-ascii" 11, 25 -- X-Winbeam-MailScanner-Information: - Please contact Technical Support for more information 11, 25 -- X-Winbeam-MailScanner: Found to be clean [] (courtesy of Winbeam) 11, 25 -- X-Winbeam-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, 11, 25 -- score=-1.11, required 4, BAYES_05 -1.11) 11, 25 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com ==============================End of - Headers==============================
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Next WEEK? Well, maybe you can be ready for next year. Microscopy Today has David Scharf's posters, and Dennis Kunkel has both a nice poster (see his website) and a really excellent book, "Hidden Worlds". Elaine Humphrey has 3 delightful 3-D (red-green) books titled "Extreme 3-D: Your Body, Scary Bugs, & Weird Animals". You can find info about the books on the MICRO website (URL below). MICRO's site also has a long hotlinked list of websites that students can explore after their visit. If you want something to print & scatter around your walls, go to MICRO's collection of quotes about microscopy.
Caroline -- Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 3, 18 -- From schooley-at-mcn.org Mon Nov 6 10:55:16 2006 3, 18 -- Received: from dns4.mcn.org (dns4.mcn.org [216.150.240.31]) 3, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA6GtFaT012561 3, 18 -- for {microscopy-at-microscopy.com} ; Mon, 6 Nov 2006 10:55:16 -0600 3, 18 -- Received: from [66.52.138.44] (helo=[10.0.1.2]) 3, 18 -- by dns4.mcn.org with esmtpa (Exim 4.60) 3, 18 -- (envelope-from {schooley-at-mcn.org} ) 3, 18 -- id J8BJ01-000BW6-4C; Mon, 06 Nov 2006 08:55:14 -0800 3, 18 -- Mime-Version: 1.0 3, 18 -- Message-Id: {a06200700c175135ef7c4-at-[10.0.1.2]} 3, 18 -- In-Reply-To: {200611060733.kA67XouC010743-at-ns.microscopy.com} 3, 18 -- References: {200611060733.kA67XouC010743-at-ns.microscopy.com} 3, 18 -- Date: Mon, 6 Nov 2006 08:47:15 -0800 3, 18 -- To: mmiralles-at-pi.ac.ae 3, 18 -- From: Caroline Schooley {schooley-at-mcn.org} 3, 18 -- Subject: Re: [Microscopy] Open Day Ideas?? 3, 18 -- Cc: microscopy-at-microscopy.com 3, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Once again, I have been dipping in to "The New Science of Strong Materials" by J. E. Gordon. For those of you who do not know, this is an absolutely wonderful book that tells at a non-technical level what materials science is all about. Thoroughly recommended if you have not read it already.
Gordon says that, in ancient Egypt, mummy cases were made by making papier mâché with papyrus. Some of the papyrus will have writing on it that might be important. On the other hand, no one would want to break up the case of a famous mummy to get pieces of papyrus that might have nothing more on them than somebody's shopping list.
I would have thought that, by now, there ought to be some form of imaging that would let us read what is on the papyrus while it is still one of many layers in the unharmed case of the mummy. I would be pleased to hear either that it has already been done, or that someone is trying to do it.
Alwyn Eades
-- ........... Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
==============================Original Headers============================== 7, 24 -- From jae5-at-lehigh.edu Mon Nov 6 13:29:32 2006 7, 24 -- Received: from rain.CC.Lehigh.EDU (rain.CC.Lehigh.EDU [128.180.39.20]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA6JTVIf026459 7, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 6 Nov 2006 13:29:31 -0600 7, 24 -- Received: from [127.0.0.1] (Dyn055141.mat.Lehigh.EDU [128.180.55.141]) 7, 24 -- (authenticated bits=0) 7, 24 -- by rain.CC.Lehigh.EDU (8.13.8/8.13.8) with ESMTP id kA6JTO5W027395 7, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 6 Nov 2006 14:29:29 -0500 7, 24 -- Message-ID: {454F8D14.1010401-at-lehigh.edu} 7, 24 -- Date: Mon, 06 Nov 2006 14:29:24 -0500 7, 24 -- From: Alwyn Eades {jae5-at-lehigh.edu} 7, 24 -- Organization: Lehigh University 7, 24 -- User-Agent: Thunderbird 1.5.0.7 (Windows/20060909) 7, 24 -- MIME-Version: 1.0 7, 24 -- To: MSA listserver {Microscopy-at-MSA.Microscopy.Com} 7, 24 -- Subject: Reading inside a solid object 7, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 24 -- Content-Transfer-Encoding: 8bit 7, 24 -- X-Virus-Scanned: ClamAV version 0.88.6, clamav-milter version 0.88.6 on rain.CC.Lehigh.EDU 7, 24 -- X-Virus-Status: Clean 7, 24 -- X-Spam-Status: No, score=-1.4 required=5.0 tests=ALL_TRUSTED 7, 24 -- autolearn=disabled version=3.1.7 7, 24 -- X-Spam-Checker-Version: SpamAssassin 3.1.7 (2006-10-05) on rain.CC.Lehigh.EDU ==============================End of - Headers==============================
I remember someone posted a message last year asking for help with the low contrast problem he was having on his cultured cells. He stated, after receiving a number of replies, that he has been using the same protocol for years and never had problem until then. I have heard a few other people reporting the same thing to me. Personally, I had an episode like that years ago, but then resolved the problem by using freshly made osmium.
Right now I am experiencing the same problem again, and the problem persisted even when I used newly ordered osmium solution (4% aqueous solution). I also used very short dehydration time to minimize potential lipid lose, but no visible improvement in contrast.
I still think this problem has something to do with osmium. But I would like to see if anyone out there has any new insight.
Thank you in advance.
Hong
Emory SOM EM
==============================Original Headers============================== 10, 25 -- From hyi-at-emory.edu Mon Nov 6 14:44:39 2006 10, 25 -- Received: from nemausa.cc.emory.edu (nemausa.cc.emory.edu [170.140.8.220]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA6KicfL006121 10, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Nov 2006 14:44:39 -0600 10, 25 -- Received: from nemausa (nemausa [170.140.8.220]) 10, 25 -- by nemausa.cc.emory.edu (8.13.8/8.13.8) with ESMTP id kA6Kicf6019792 10, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Nov 2006 15:44:38 -0500 (EST) 10, 25 -- Received: from nemausa (unknown [127.0.0.1]) 10, 25 -- by nemausa (Symantec Mail Security) with ESMTP id 632A824F6 10, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Nov 2006 15:44:38 -0500 (EST) 10, 25 -- X-AuditID: aa8c08dc-000000090000171d-06-454f9eb6747b 10, 25 -- Received: from [170.140.233.153] (dhcp233153.wmb.emory.edu [170.140.233.153]) 10, 25 -- by nemausa (Symantec Mail Security) with ESMTP id 326BF24F0 10, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Nov 2006 15:44:38 -0500 (EST) 10, 25 -- Mime-Version: 1.0 (Apple Message framework v622) 10, 25 -- Message-Id: {d6203d1f421b9c295156fd4808ea80b7-at-emory.edu} 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 25 -- To: Microscopy-at-microscopy.com 10, 25 -- From: Hong Yi {hyi-at-emory.edu} 10, 25 -- Subject: (Microscopy) Low contrast 10, 25 -- Date: Mon, 6 Nov 2006 15:44:33 -0500 10, 25 -- X-Mailer: Apple Mail (2.622) 10, 25 -- X-Brightmail-Tracker: AAAAAA== 10, 25 -- Content-Transfer-Encoding: 8bit 10, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA6KicfL006121 ==============================End of - Headers==============================
Listers, I have just been given a Nanotech Plasmaprep 100 "Plasma Chemistry Unit" that may or may not be functional. It looks to be complete other than a vacuum pump. Does anyone have a manual or wiring diagrams for this unit? Actually, even someone's operating procedures would be better than nothing. Thanks for helping. Kim
-- Kim Rensing Ph.D. Manager, Microscopy and Imaging Facility
University of Calgary, Health Sciences Centre B129 - 3330 Hospital Drive NW Calgary, AB, Canada T2N 4N1
403-220-3488 krensing-at-ucalgary.ca
==============================Original Headers============================== 4, 25 -- From krensing-at-ucalgary.ca Mon Nov 6 16:56:27 2006 4, 25 -- Received: from mr2.ucalgary.ca (mr2.ucalgary.ca [136.159.34.166]) 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA6MuRPI019074 4, 25 -- for {microscopy-at-microscopy.com} ; Mon, 6 Nov 2006 16:56:27 -0600 4, 25 -- Received: from smtp3.ucalgary.ca (smtp3.ucalgary.ca [136.159.34.64]) 4, 25 -- by mr2.ucalgary.ca (Postfix) with ESMTP id 268B63669C 4, 25 -- for {microscopy-at-microscopy.com} ; Mon, 6 Nov 2006 15:56:26 -0700 (MST) 4, 25 -- Received: from [192.168.0.100] ([136.159.164.171]) 4, 25 -- (authenticated (0 bits)) 4, 25 -- by smtp3.ucalgary.ca (8.11.7/8.11.6) with ESMTP id kA6MuNc01446 4, 25 -- (using TLSv1/SSLv3 with cipher DHE-RSA-AES256-SHA (256 bits) verified NO) 4, 25 -- for {microscopy-at-microscopy.com} ; Mon, 6 Nov 2006 15:56:23 -0700 4, 25 -- Message-ID: {454FBD92.1020404-at-ucalgary.ca} 4, 25 -- Date: Mon, 06 Nov 2006 15:56:18 -0700 4, 25 -- From: Kim Rensing {krensing-at-ucalgary.ca} 4, 25 -- Organization: Microscopy and Imaging Facility, U. of Calgary 4, 25 -- User-Agent: Thunderbird 1.5.0.7 (Windows/20060909) 4, 25 -- MIME-Version: 1.0 4, 25 -- To: microscopy-at-microscopy.com 4, 25 -- Subject: nanotech plasmaprep 100 4, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 25 -- Content-Transfer-Encoding: 7bit 4, 25 -- X-UCalgary-MailScanner-Information: Please contact IT Help Desk at (403) 220-5555 for more information 4, 25 -- X-UCalgary-MailScanner: Found to be clean 4, 25 -- X-UCalgary-MailScanner-From: krensing-at-ucalgary.ca ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both klangwor-at-uoregon.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: klangwor-at-uoregon.edu Name: Kurt Langworhty
Organization: Univeristy of Oregon
Title-Subject: [Filtered] Surplus Equipment
Question: We are consolidating our confocal and EM labs to prepare for the construction of our Integrated Sciences Building.
I need to donate/sell some equipment to make room in the lab. I'm trying to find homes for:
Varian VE-10 Vacuum Evaporator
Hughes Laser Defractometer (with laser power supply included)
7 boxes of 4x5in Polaroid 55 SEM film (20/box)
Possibly more to come...
Any interested parties should contact me by email (klangwor-at-uoregon.edu)
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both zhang.zaoli-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: zhang.zaoli-at-gmail.com Name: Zaoli
Organization: Ulm univsersity
Title-Subject: [Filtered] TEM specimen preparation about Li compound
Question: Hi,
I am going to carry out the TEM investigation on Li compound single crystals, i.e. LiCoO, or LiFePO4 etc .. I am afraid that routine TEM sample preparation may bring the damage or remove active Li atoms from the sample. does anyone have experience or suggestions on how to prepare TEM samples of such Li compound crystals ? and what kind of damgage could be introduced if a routine TEM sample preparation used ?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pavi_micro-at-yahoo.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, November 6, 2006 at 23:57:52 ---------------------------------------------------------------------------
As a material science characterizations engineer with 5 years of work experience, I am searching for a SEM / TEM / SIMS / X-Ray microtomography position, in a laboratory or industrial lab. I have good skills in Electron Microscopy (materials analysis) and in X-Ray microtomography systems. Please ask me (mail adress : samuel_meulenyzer-at-yahoo.fr) my Curriculum Vitae and more details if you are intersted.
As an european citizen, I am free to work anywhere.
Thanks very much.
Samuel Meulenyzer
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==============================Original Headers============================== 11, 19 -- From samuel_meulenyzer-at-yahoo.fr Tue Nov 7 08:43:17 2006 11, 19 -- Received: from web27901.mail.ukl.yahoo.com (web27901.mail.ukl.yahoo.com [217.146.182.51]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kA7EhGbd025238 11, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Nov 2006 08:43:17 -0600 11, 19 -- Received: (qmail 85797 invoked by uid 60001); 7 Nov 2006 14:43:15 -0000 11, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 19 -- s=s1024; d=yahoo.fr; 11, 19 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 11, 19 -- b=zRvrQQoqkGTTnTqE7mh4hC90U/MgIPSjRyIVjf62AWYvMhljB3KdhONZG3DqbzuTvzHQreEXPeNpxIQ6yd/Z+8436Fx1uR8Q63DLwArLFHpfg+HaxNJ7FFggwzj7FDq9GmBDRGNCZ06pAK1zJcXCW9Isv5834zI4GvD7gKeqQtk= ; 11, 19 -- Message-ID: {20061107144315.85795.qmail-at-web27901.mail.ukl.yahoo.com} 11, 19 -- Received: from [193.190.208.38] by web27901.mail.ukl.yahoo.com via HTTP; Tue, 07 Nov 2006 14:43:15 GMT 11, 19 -- Date: Tue, 7 Nov 2006 14:43:15 +0000 (GMT) 11, 19 -- From: Samuel Meulenyzer {samuel_meulenyzer-at-yahoo.fr} 11, 19 -- Subject: [Microscopy] SEM (EDX + WDX) - TEM - X-Ray microtomography - Engineer in material characterizations, micro analysis technique 11, 19 -- To: Microscopy-at-microscopy.com 11, 19 -- MIME-Version: 1.0 11, 19 -- Content-Type: text/plain; charset=iso-8859-1 11, 19 -- Content-Transfer-Encoding: 8bit 11, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA7EhGbd025238 ==============================End of - Headers==============================
A Dark field scope produces an image of brightly illuminated objects on a dark background. It was one of the earliest attempts to increase the resolution and detectability of objects.
Abbe's theory of resolution states that for good resolution you need to capture two of three possible rays. This is assuming that each microscopic object acts as a diffraction grating. This isn't too bad of an assumption. The three rays are one direct, undeviated ray from the sample and two primary diffracted rays. (We like to think of these thought experiment and ray diagrams has two dimensional drawing on a sheet of paper). For some subjects the diffracted rays fall outside of the light accepting ability of the objective and these objects can not be resolved. (Bummer!)
One approach is to tilt or move the condense off center (Many of the old medical grade scopes had this ability). This allows the undeviated ray and one diffracted ray to enter the front lens of the objective. This produces a dark background and white illuminated subjects.
A second approach is to use a central stop in the condenser to block the central ray and allow only highly angled rays to illuminated the subject. The diffracted rays from these angled light rays form the image.
You often need a powerful light source or work in a very dark room with dark adapted eyes because you are throwing away a lot of light with a central stop. Special condensers were, and I believe, are still made to efficiently produce darkfield illumination.
Newer illuminating systems (like phase contrast) have replaced a lot of darkfield work. Still some incredible images can be formed with darkfield. One easy short cut is to use a phase contrast scope and select a condenser phase ring larger than the ring in your objective.
Best wishes...........
Frank Karl Degussa Coproration
pavi_micro-at-yahoo. com To: frank.karl-at-degussa.com cc: 11/07/2006 08:12 Subject: [Microscopy] AskAMicroscopist: dark field AM Please respond to pavi_micro
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pavi_micro-at-yahoo.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, November 6, 2006 at 23:57:52 ---------------------------------------------------------------------------
Microscopists, While Frank Karl is undoubtedly correct that the spur to invent dark field was based on resolution and tilting beams, I wanted to mention that there is a useful rubric for classifying types of microscopy, and that is based on contrast. Like this:
In each case, the microscope takes advantage of a specific kind of interaction between light and matter to generate contrast (of course there are overlaps, eg scattering contributes to contrast in a brightfield microscope, etc).
(The Nomarski one may be hard to understand, this kind of optics gives rise to a signal whenever the gradient in optical path in a local region is different than what the gradient is in the background).
Hope this helps, Tobias
} } } What an interesting question. } } A Dark field scope produces an image of brightly illuminated objects on a } dark background. It was one of the earliest attempts to increase the } resolution and detectability of objects. } } Abbe's theory of resolution states that for good resolution you need to } capture two of three possible rays. This is assuming that each microscopic } object acts as a diffraction grating. This isn't too bad of an assumption. } The three rays are one direct, undeviated ray from the sample and two } primary diffracted rays. (We like to think of these thought experiment and } ray diagrams has two dimensional drawing on a sheet of paper). For some } subjects the diffracted rays fall outside of the light accepting ability of } the objective and these objects can not be resolved. (Bummer!) } } One approach is to tilt or move the condense off center (Many of the old } medical grade scopes had this ability). This allows the undeviated ray and } one diffracted ray to enter the front lens of the objective. This produces } a dark background and white illuminated subjects. } } A second approach is to use a central stop in the condenser to block the } central ray and allow only highly angled rays to illuminated the subject. } The diffracted rays from these angled light rays form the image. } } You often need a powerful light source or work in a very dark room with } dark adapted eyes because you are throwing away a lot of light with a } central stop. Special condensers were, and I believe, are still made to } efficiently produce darkfield illumination. } } Newer illuminating systems (like phase contrast) have replaced a lot of } darkfield work. Still some incredible images can be formed with darkfield. } One easy short cut is to use a phase contrast scope and select a condenser } phase ring larger than the ring in your objective. } } Best wishes........... } } Frank Karl } Degussa Coproration } } } } } } } pavi_micro-at-yahoo. } com To: } frank.karl-at-degussa.com } } cc: } 11/07/2006 08:12 Subject: } [Microscopy] AskAMicroscopist: dark } field } } AM } Please respond } to } } pavi_micro } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Did anyone has an experience with LR White polymerization at the temperature range 45-55C? Could you, please, suggest the appropriate embedding/curing protocol and your vendor of choice? My protocol involves the cryofixation, cryo substitution with acetone (aldehydes or osmium fixatives are no-no). The UV polymerization could be difficult to achieve due to the technical limitations; and temperatures exceeding 55C are damaging for some of the structures. The 46-49C range is absolutely preferred. Thank you in advance, Albina
MIKHAYLOVA,ALBINA, PhD Materials Science and Engineering University of Florida PO Box 116400 Gainesville, Florida 32611 Phone: (352) 392-6533 Fax: (352) 392-3771 E-Mail: amich-at-ufl.edu
==============================Original Headers============================== 5, 22 -- From amich-at-ufl.edu Tue Nov 7 10:09:18 2006 5, 22 -- Received: from smtp.ufl.edu (smtp02.osg.ufl.edu [128.227.74.165]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA7G9I88004236 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Nov 2006 10:09:18 -0600 5, 22 -- Received: from osgjas02.cns.ufl.edu (osgjas02.cns.ufl.edu [128.227.74.132]) 5, 22 -- by smtp.ufl.edu (8.13.7/8.13.7/2.5.9) with ESMTP id kA7G992M1474576 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Nov 2006 11:09:10 -0500 5, 22 -- Message-ID: {1156372807.74371162915749374.JavaMail.osg-at-osgjas02.cns.ufl.edu} 5, 22 -- Date: Tue, 7 Nov 2006 11:09:09 -0500 (EST) 5, 22 -- From: "MIKHAYLOVA,ALBINA" {amich-at-ufl.edu} 5, 22 -- To: Microscopy-at-microscopy.com 5, 22 -- Subject: LR white question 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: text/plain; format=flowed; charset=us-ascii 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-Mailer: GatorMail WebMail (http://GatorMail.sf.net/) 5, 22 -- X-Originating-IP: 72.155.89.76 [72.155.89.76] 5, 22 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0rc3 (smtp.ufl.edu [128.227.74.56]); Tue, 07 Nov 2006 11:09:10 -0500 (EST) 5, 22 -- X-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 5, 22 -- X-UFL-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 5, 22 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 5, 22 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America Hi Alwyn,
I wonder if terahertz microwave imaging might work?
Airport security are experimenting with this in the UK - apparently, it can image, in real time, underneath people's clothes to see if hidden weapons, etc, are being carried. Seems a lot more sensitive and controllable than X-rays. Various concerns about security staff having fun watching 'naked' passengers ....
Perhaps some careful tuning would make it possible image at various depths and select different materials to 'see' this writing ...? -- Larry Stoter
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==============================Original Headers============================== 6, 18 -- From larry-at-cymru.freewire.co.uk Tue Nov 7 14:33:07 2006 6, 18 -- Received: from get.freewire.net (get.freewire.net [86.54.106.202] (may be forged)) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA7KX646030998 6, 18 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 7 Nov 2006 14:33:06 -0600 6, 18 -- Received: from [217.154.253.197] (th6dc-217-154-253-197.dial.mistral.co.uk [217.154.253.197]) 6, 18 -- by get.freewire.net (8.13.6/8.11.6) with ESMTP id kA7KWqDu017101; 6, 18 -- Tue, 7 Nov 2006 20:32:57 GMT 6, 18 -- Mime-Version: 1.0 6, 18 -- Message-Id: {p06210201c1769be24691-at-[217.154.250.120]} 6, 18 -- In-Reply-To: {200611061931.kA6JVuRr028680-at-ns.microscopy.com} 6, 18 -- References: {200611061931.kA6JVuRr028680-at-ns.microscopy.com} 6, 18 -- Date: Tue, 7 Nov 2006 20:28:44 +0000 6, 18 -- To: jae5-at-lehigh.edu, Microscopy-at-MSA.Microscopy.Com 6, 18 -- From: Larry Stoter {larry-at-cymru.freewire.co.uk} 6, 18 -- Subject: Re: [Microscopy] Reading inside a solid object 6, 18 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 6, 18 -- Content-Transfer-Encoding: 8bit 6, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA7KX646030998 ==============================End of - Headers==============================
My question is: What type of commercially available membrane such as a type of millipore filter could be used as a substrate to grow cells (keratinocytes) and subsequently fix, cryoprotect and ultrathin cryosection the membrane/cells intact? If anyone has done this, I would certainly appreciate some advice.
Robert Underwood Universtiy of Washington Dermatology
==============================Original Headers============================== 4, 21 -- From underwoo-at-u.washington.edu Tue Nov 7 15:45:12 2006 4, 21 -- Received: from mxout7.cac.washington.edu (mxout7.cac.washington.edu [140.142.32.178]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA7LjCYE010615 4, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 7 Nov 2006 15:45:12 -0600 4, 21 -- Received: from hymn10.u.washington.edu (hymn10.u.washington.edu [140.142.13.244]) 4, 21 -- by mxout7.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW06.09) with ESMTP id kA7LjB57010911 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 7 Nov 2006 13:45:11 -0800 4, 21 -- Received: from localhost (localhost [127.0.0.1]) 4, 21 -- by hymn10.u.washington.edu (8.13.7+UW06.06/8.13.7+UW06.09) with ESMTP id kA7LjBZF010953 4, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 7 Nov 2006 13:45:11 -0800 4, 21 -- X-Auth-Received: from [128.208.106.163] by hymn10.u.washington.edu via HTTP; Tue, 07 Nov 2006 13:45:11 PST 4, 21 -- Date: Tue, 7 Nov 2006 13:45:11 -0800 (PST) 4, 21 -- From: Robert A Underwood {underwoo-at-u.washington.edu} 4, 21 -- To: Microscopy List {Microscopy-at-MSA.Microscopy.Com} 4, 21 -- Subject: [Microscopy] cryothin sectioning millipore filter 4, 21 -- Message-ID: {Pine.LNX.4.43.0611071345110.13269-at-hymn10.u.washington.edu} 4, 21 -- MIME-Version: 1.0 4, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 4, 21 -- X-PMX-Version: 5.2.1.279297, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2006.11.7.132933 4, 21 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
A few more Comments on Darkfield - but I like the contrast comparison.
1. Part I - Darkfield history
Darkfield has been around a long time (1800s), including paraboloid and ellipsoid condenser uses.
Classically done with a stop in the condenser or with a reflecting objective.
But can be done with a stop on the objective, at the light source, or another focal plane before the eye.
Modifications of this are:
A) with dispersion staining [promoted by Crossman as well as Brown and McCrone in the 1960s, but actually first described by Dodge in Am. Min. in 1948]
or
B) critical darkfield (you can look up an article by Ted Clarke in Microscopy Today on critical Darkfield which comes from: Havics & Clarke: “Critical Darkfield and Its Application to Asbestos Analysis” presented at Inter/Micro-2002, June 24-27, 2002, Chicago, IL.; there are some nice diagrams of the process of light interaction)
2. Part II - Contrast Issues
Phase
I(x’) proportional to [1 - (2*(phase dif)(x’)) ] I = intensity Thus linear with phase change (RI*thickness) useful for up to 1/2 wavelength Best at {1/10 wavelength
Darkfield
I(x’) proportional to (phase dif)^2 (ingoring secondary terms) Thus not so linear with phase change And useful for outlines or very small particles/differences
Hoffman Modulation Contrast
dI = If*(Phase dif)*(TB - TG)*2cos(theta/w) TB = transmittance in bright area TG = transmittance in gray area W = width of slit Theta = angle from perpedicular to slit Thus non-linear but smoothed
Normaski DIC
I proportional to d(dif phase)/dx (simplified) useful from } 1/10 wavelength Up to 1 wavelength best above 1/2 wavelength
[Source for above: Havics: “Asbestos Fiber Counting by Different Optical Contrast Techniques”, presented at Inter/Micro-2003, July 7-10, 2003, Chicago, IL.; I'm trying to get an article out on this in the next 3 months or so which is why it's at my fingertips]
3. Part III additional Comments
Resolution of Darkfield} Hoffman} Phase } DIC } Brightfield based on my tests using a resolution test slide (HSE/NPL test slide). Fluorescence is too media dependent to make a broad statement but does better than Hoffman using fluorescent spheres and about the same as darkfield.
Resolution does not necessarily mean detectability. Contrast is better for detectability of an object whereas resolution is better for defining the structure once detected.
A Dark background in general increases detectability vs a bright background.
Tony
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-----Original Message----- X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] Sent: Tuesday, November 07, 2006 9:55 AM To: ph2-at-sprynet.com
Microscopists, While Frank Karl is undoubtedly correct that the spur to invent dark field was based on resolution and tilting beams, I wanted to mention that there is a useful rubric for classifying types of microscopy, and that is based on contrast. Like this:
In each case, the microscope takes advantage of a specific kind of interaction between light and matter to generate contrast (of course there are overlaps, eg scattering contributes to contrast in a brightfield microscope, etc).
(The Nomarski one may be hard to understand, this kind of optics gives rise to a signal whenever the gradient in optical path in a local region is different than what the gradient is in the background).
Hope this helps, Tobias
} } } What an interesting question. } } A Dark field scope produces an image of brightly illuminated objects } on a dark background. It was one of the earliest attempts to increase } the resolution and detectability of objects. } } Abbe's theory of resolution states that for good resolution you need to } capture two of three possible rays. This is assuming that each } microscopic object acts as a diffraction grating. This isn't too bad } of an assumption. The three rays are one direct, undeviated ray from } the sample and two primary diffracted rays. (We like to think of these } thought experiment and ray diagrams has two dimensional drawing on a } sheet of paper). For some subjects the diffracted rays fall outside of } the light accepting ability of the objective and these objects can not } be resolved. (Bummer!) } } One approach is to tilt or move the condense off center (Many of the } old medical grade scopes had this ability). This allows the undeviated } ray and one diffracted ray to enter the front lens of the objective. } This produces a dark background and white illuminated subjects. } } A second approach is to use a central stop in the condenser to block } the central ray and allow only highly angled rays to illuminated the } subject. The diffracted rays from these angled light rays form the } image. } } You often need a powerful light source or work in a very dark room with } dark adapted eyes because you are throwing away a lot of light with a } central stop. Special condensers were, and I believe, are still made } to efficiently produce darkfield illumination. } } Newer illuminating systems (like phase contrast) have replaced a lot of } darkfield work. Still some incredible images can be formed with } darkfield. One easy short cut is to use a phase contrast scope and } select a condenser phase ring larger than the ring in your objective. } } Best wishes........... } } Frank Karl } Degussa Coproration } } } } } } } pavi_micro-at-yahoo. } com To: } frank.karl-at-degussa.com } } cc: } 11/07/2006 08:12 Subject: } [Microscopy] AskAMicroscopist: dark } field } } AM } Please respond } to } } pavi_micro } } } } } } } } } } ----------------------------------------------------------------------- } ----- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
Does anyone know anything about this techinique for gradient shaving of thin films to expose the surface for FTIR and other analysis? I was approched about using my microtome for this, but from what I can gather about this machine it is much more specialized, and can measure forces experienced by the knife during cutting. They are only interested in exposing the face for analyis, so I am wondering what kind of knife is used, how the alignment is done, etc. Any information would be appreciated. The citation I have in a couple papers is for a machine by Daipla Wintes, an SAICAS CN-20.
Thanks, Leslie Krupp _____________________ Leslie Krupp IBM Almaden Research 650 Harry Road, K19/D1 San Jose, CA 95120-6099 (408) 927-3856
==============================Original Headers============================== 3, 34 -- From lkrupp-at-us.ibm.com Tue Nov 7 17:17:07 2006 3, 34 -- Received: from e4.ny.us.ibm.com (e4.ny.us.ibm.com [32.97.182.144]) 3, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA7NH75a004488 3, 34 -- for {microscopy-at-microscopy.com} ; Tue, 7 Nov 2006 17:17:07 -0600 3, 34 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236]) 3, 34 -- by e4.ny.us.ibm.com (8.13.8/8.12.11) with ESMTP id kA7NH7Pn008146 3, 34 -- for {microscopy-at-microscopy.com} ; Tue, 7 Nov 2006 18:17:07 -0500 3, 34 -- Received: from d01av01.pok.ibm.com (d01av01.pok.ibm.com [9.56.224.215]) 3, 34 -- by d01relay04.pok.ibm.com (8.13.6/8.13.6/NCO v8.1.1) with ESMTP id kA7NH6g3150320 3, 34 -- for {microscopy-at-microscopy.com} ; Tue, 7 Nov 2006 18:17:06 -0500 3, 34 -- Received: from d01av01.pok.ibm.com (loopback [127.0.0.1]) 3, 34 -- by d01av01.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id kA7NH6rr030368 3, 34 -- for {microscopy-at-microscopy.com} ; Tue, 7 Nov 2006 18:17:06 -0500 3, 34 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) 3, 34 -- by d01av01.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id kA7NH6Wh030365 3, 34 -- for {microscopy-at-microscopy.com} ; Tue, 7 Nov 2006 18:17:06 -0500 3, 34 -- To: microscopy-at-microscopy.com 3, 34 -- MIME-Version: 1.0 3, 34 -- Subject: Surface and Interfacial Cutting Analyis System? 3, 34 -- X-Mailer: Lotus Notes Release 7.0 HF277 June 21, 2006 3, 34 -- Message-ID: {OF62816355.46492074-ON8525721F.007FCF82-8825721F.007FEE8C-at-us.ibm.com} 3, 34 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} 3, 34 -- Date: Tue, 7 Nov 2006 18:16:59 -0500 3, 34 -- X-MIMETrack: S/MIME Sign by Notes Client on Leslie E Krupp/Almaden/IBM(Release 7.0 HF277|June 3, 34 -- 21, 2006) at 11/07/2006 03:17:21 PM, 3, 34 -- Serialize by Notes Client on Leslie E Krupp/Almaden/IBM(Release 7.0 HF277|June 3, 34 -- 21, 2006) at 11/07/2006 03:17:21 PM, 3, 34 -- Serialize complete at 11/07/2006 03:17:21 PM, 3, 34 -- S/MIME Sign failed at 11/07/2006 03:17:21 PM: The cryptographic key was not 3, 34 -- found, 3, 34 -- Serialize by Router on D01ML604/01/M/IBM(Release 7.0.2HF32 | October 17, 2006) at 3, 34 -- 11/07/2006 18:17:05, 3, 34 -- Serialize complete at 11/07/2006 18:17:05 3, 34 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
It is not easy to find a heat pen in this small isolated country named Austria ;-). The only one I found available is around 600 euros, which I find a LITTLE BIT expensive for such an instrument. Someone told me that a soldering iron would do the trick, and they are available at 10 euros in every general store. Has anyone experienced this electrician tool for EM work? Or perhaps someone has an idea where I could find a "true" heat pen at fair price in our old continent?
Regards,
Stephane
==============================Original Headers============================== 7, 19 -- From nizets2-at-yahoo.com Wed Nov 8 01:24:23 2006 7, 19 -- Received: from web37412.mail.mud.yahoo.com (web37412.mail.mud.yahoo.com [209.191.91.144]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kA87ONDc024130 7, 19 -- for {microscopy-at-microscopy.com} ; Wed, 8 Nov 2006 01:24:23 -0600 7, 19 -- Received: (qmail 19776 invoked by uid 60001); 8 Nov 2006 07:24:22 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 7, 19 -- b=CD/Knw0zoRhb0W0iMDibqVsUDkXBXPEl3Qh+txTK/Cr61x4Ncby+KJycSzmyJQDaWSvJLn+8yvk/mjn1mZff9q2+5umgUs4dJKAN8TokIMfik1JsS1gH4s1Nw8fhXB64ufi+djyUMhGvloAR/hit5BIpsAaQON7mVb6JCiQdQPE= ; 7, 19 -- Message-ID: {20061108072422.19774.qmail-at-web37412.mail.mud.yahoo.com} 7, 19 -- Received: from [80.122.101.102] by web37412.mail.mud.yahoo.com via HTTP; Tue, 07 Nov 2006 23:24:22 PST 7, 19 -- Date: Tue, 7 Nov 2006 23:24:22 -0800 (PST) 7, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 19 -- Subject: Heat pen 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=ascii 7, 19 -- Content-Transfer-Encoding: 8bit 7, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA87ONDc024130 ==============================End of - Headers==============================
Recently I tried successfully the following post-fixation:
3mls h2O 1ml 4% OsO4 0.03grams KFECN (329.26)
I warmly recommend it to any TEM microscopist. It is very easy to prepare and can only improve your image. I really improved my membrane staining, and I think it could improve the staining of glycogen too, but I need more time to appreciate that point.
Regards,
Stephane
----- Original Message ---- X-from: "hyi-at-emory.edu" {hyi-at-emory.edu} To: nizets2-at-yahoo.com Sent: Monday, November 6, 2006 9:49:54 PM
Dear Microscopy Researchers:
I remember someone posted a message last year asking for help with the low contrast problem he was having on his cultured cells. He stated, after receiving a number of replies, that he has been using the same protocol for years and never had problem until then. I have heard a few other people reporting the same thing to me. Personally, I had an episode like that years ago, but then resolved the problem by using freshly made osmium.
Right now I am experiencing the same problem again, and the problem persisted even when I used newly ordered osmium solution (4% aqueous solution). I also used very short dehydration time to minimize potential lipid lose, but no visible improvement in contrast.
I still think this problem has something to do with osmium. But I would like to see if anyone out there has any new insight.
Thank you in advance.
Hong
Emory SOM EM
==============================Original Headers============================== 10, 25 -- From hyi-at-emory.edu Mon Nov 6 14:44:39 2006 10, 25 -- Received: from nemausa.cc.emory.edu (nemausa.cc.emory.edu [170.140.8.220]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA6KicfL006121 10, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Nov 2006 14:44:39 -0600 10, 25 -- Received: from nemausa (nemausa [170.140.8.220]) 10, 25 -- by nemausa.cc.emory.edu (8.13.8/8.13.8) with ESMTP id kA6Kicf6019792 10, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Nov 2006 15:44:38 -0500 (EST) 10, 25 -- Received: from nemausa (unknown [127.0.0.1]) 10, 25 -- by nemausa (Symantec Mail Security) with ESMTP id 632A824F6 10, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Nov 2006 15:44:38 -0500 (EST) 10, 25 -- X-AuditID: aa8c08dc-000000090000171d-06-454f9eb6747b 10, 25 -- Received: from [170.140.233.153] (dhcp233153.wmb.emory.edu [170.140.233.153]) 10, 25 -- by nemausa (Symantec Mail Security) with ESMTP id 326BF24F0 10, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Nov 2006 15:44:38 -0500 (EST) 10, 25 -- Mime-Version: 1.0 (Apple Message framework v622) 10, 25 -- Message-Id: {d6203d1f421b9c295156fd4808ea80b7-at-emory.edu} 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 25 -- To: Microscopy-at-microscopy.com 10, 25 -- From: Hong Yi {hyi-at-emory.edu} 10, 25 -- Subject: (Microscopy) Low contrast 10, 25 -- Date: Mon, 6 Nov 2006 15:44:33 -0500 10, 25 -- X-Mailer: Apple Mail (2.622) 10, 25 -- X-Brightmail-Tracker: AAAAAA== 10, 25 -- Content-Transfer-Encoding: 8bit 10, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA6KicfL006121 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 20 -- From nizets2-at-yahoo.com Wed Nov 8 02:00:41 2006 24, 20 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138]) 24, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kA880f9x002864 24, 20 -- for {microscopy-at-microscopy.com} ; Wed, 8 Nov 2006 02:00:41 -0600 24, 20 -- Received: (qmail 6745 invoked by uid 60001); 8 Nov 2006 08:00:41 -0000 24, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 24, 20 -- s=s1024; d=yahoo.com; 24, 20 -- h=Message-ID:Received:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding; 24, 20 -- b=m+tVKJoxJmZo6qB1S4gez17fcczfstvUoxpjKJVS/Hvuh6ydfK2n6MnFxe0yERGWESlZk0LGRrvCiv2OOtmc5nwoAta7wyT9J58vagBfzwlkq126KmYXy2IjMQ12+4dUpEEnuedt/QGKJzzJZH83U1c8bk0IFGCxuPevLif3908= ; 24, 20 -- Message-ID: {20061108080041.6743.qmail-at-web37406.mail.mud.yahoo.com} 24, 20 -- Received: from [80.122.101.102] by web37406.mail.mud.yahoo.com via HTTP; Wed, 08 Nov 2006 00:00:41 PST 24, 20 -- Date: Wed, 8 Nov 2006 00:00:41 -0800 (PST) 24, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 24, 20 -- Subject: Re: [Microscopy] (Microscopy) Low contrast 24, 20 -- To: hyi-at-emory.edu 24, 20 -- Cc: microscopy-at-microscopy.com 24, 20 -- MIME-Version: 1.0 24, 20 -- Content-Type: text/plain; charset=ascii 24, 20 -- Content-Transfer-Encoding: 8bit 24, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA880f9x002864 ==============================End of - Headers==============================
Hi Tony, I thought about dispersion staining, but...
With darkfield we are attempting to manipulate the sample and illumination to get the best possible combination of resolution and detectability. The central stop (or annular) in the back focal plane of the objective helps increase the detectability of the dispersion of light in the sample and mounting media at the expense of resolution. All of the diffracted, image forming rays from the sample have been captured by the front lens. Manipulation of these image forming rays in the back focal plan can increase detectability, add color, make for powerful striking images, but I suspect no further improvement in resolution is possible. I've looked at a lot of chrysotile (as I sure you have) and I've detected very small amounts, but I have never seen an increase in resolution in dispersion staining darkfield or annular stop as compared to good kohler illumination.
Best wishes.....Frank
PS: good luck with the article!!!
ph2-at-sprynet.com To: frank.karl-at-degussa.com 11/07/2006 03:59 cc: PM Subject: [Microscopy] RE: dark field and beyond Please respond to ph2
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
A few more Comments on Darkfield - but I like the contrast comparison.
1. Part I - Darkfield history
Darkfield has been around a long time (1800s), including paraboloid and ellipsoid condenser uses.
Classically done with a stop in the condenser or with a reflecting objective.
But can be done with a stop on the objective, at the light source, or another focal plane before the eye.
Modifications of this are:
A) with dispersion staining [promoted by Crossman as well as Brown and McCrone in the 1960s, but actually first described by Dodge in Am. Min. in 1948]
or
B) critical darkfield (you can look up an article by Ted Clarke in Microscopy Today on critical Darkfield which comes from: Havics & Clarke: “Critical Darkfield and Its Application to Asbestos Analysis” presented at Inter/Micro-2002, June 24-27, 2002, Chicago, IL.; there are some nice diagrams of the process of light interaction)
2. Part II - Contrast Issues
Phase
I(x’) proportional to [1 - (2*(phase dif)(x’)) ] I = intensity Thus linear with phase change (RI*thickness) useful for up to 1/2 wavelength Best at {1/10 wavelength
Darkfield
I(x’) proportional to (phase dif)^2 (ingoring secondary terms) Thus not so linear with phase change And useful for outlines or very small particles/differences
Hoffman Modulation Contrast
dI = If*(Phase dif)*(TB - TG)*2cos(theta/w) TB = transmittance in bright area TG = transmittance in gray area W = width of slit Theta = angle from perpedicular to slit Thus non-linear but smoothed
Normaski DIC
I proportional to d(dif phase)/dx (simplified) useful from } 1/10 wavelength Up to 1 wavelength best above 1/2 wavelength
[Source for above: Havics: “Asbestos Fiber Counting by Different Optical Contrast Techniques”, presented at Inter/Micro-2003, July 7-10, 2003, Chicago, IL.; I'm trying to get an article out on this in the next 3 months or so which is why it's at my fingertips]
3. Part III additional Comments
Resolution of Darkfield} Hoffman} Phase } DIC } Brightfield based on my tests using a resolution test slide (HSE/NPL test slide). Fluorescence is too media dependent to make a broad statement but does better than Hoffman using fluorescent spheres and about the same as darkfield.
Resolution does not necessarily mean detectability. Contrast is better for detectability of an object whereas resolution is better for defining the structure once detected.
A Dark background in general increases detectability vs a bright background.
Tony
.......................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC PO Box 34140 Indianapolis, IN 46234 (317) 752-6386 (317) 409-3238 cell
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-----Original Message----- X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] Sent: Tuesday, November 07, 2006 9:55 AM To: ph2-at-sprynet.com
Microscopists, While Frank Karl is undoubtedly correct that the spur to invent dark field was based on resolution and tilting beams, I wanted to mention that there is a useful rubric for classifying types of microscopy, and that is based on contrast. Like this:
In each case, the microscope takes advantage of a specific kind of interaction between light and matter to generate contrast (of course there are overlaps, eg scattering contributes to contrast in a brightfield microscope, etc).
(The Nomarski one may be hard to understand, this kind of optics gives rise to a signal whenever the gradient in optical path in a local region is different than what the gradient is in the background).
Hope this helps, Tobias
} } } What an interesting question. } } A Dark field scope produces an image of brightly illuminated objects } on a dark background. It was one of the earliest attempts to increase } the resolution and detectability of objects. } } Abbe's theory of resolution states that for good resolution you need to } capture two of three possible rays. This is assuming that each } microscopic object acts as a diffraction grating. This isn't too bad } of an assumption. The three rays are one direct, undeviated ray from } the sample and two primary diffracted rays. (We like to think of these } thought experiment and ray diagrams has two dimensional drawing on a } sheet of paper). For some subjects the diffracted rays fall outside of } the light accepting ability of the objective and these objects can not } be resolved. (Bummer!) } } One approach is to tilt or move the condense off center (Many of the } old medical grade scopes had this ability). This allows the undeviated } ray and one diffracted ray to enter the front lens of the objective. } This produces a dark background and white illuminated subjects. } } A second approach is to use a central stop in the condenser to block } the central ray and allow only highly angled rays to illuminated the } subject. The diffracted rays from these angled light rays form the } image. } } You often need a powerful light source or work in a very dark room with } dark adapted eyes because you are throwing away a lot of light with a } central stop. Special condensers were, and I believe, are still made } to efficiently produce darkfield illumination. } } Newer illuminating systems (like phase contrast) have replaced a lot of } darkfield work. Still some incredible images can be formed with } darkfield. One easy short cut is to use a phase contrast scope and } select a condenser phase ring larger than the ring in your objective. } } Best wishes........... } } Frank Karl } Degussa Coproration } } } } }
} } pavi_micro-at-yahoo.
} com To: } frank.karl-at-degussa.com } } cc:
} 11/07/2006 08:12 Subject: } [Microscopy] AskAMicroscopist: dark } field } } AM
} Please respond } to
} } pavi_micro
} }
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} } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (pavi_micro-at-yahoo.com) from } http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on } Monday, November 6, 2006 at 23:57:52 } ----------------------------------------------------------------------- } ---- } } Email: pavi_micro-at-yahoo.com } Name: mrs.pavitra jain } } Organization: Microbiologist } } Education: Graduate College } } Location: Hubli,India } } Question: What is dark field microscope } } ----------------------------------------------------------------------- } ---- } } ==============================Original } Headers============================== } 7, 12 -- From zaluzec-at-ultra5.microscopy.com Tue Nov 7 08:10:19 2006 7, } 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id kA7EAJO5003959 } 7, 12 -- for {microscopy-at-microscopy.com} ; Tue, 7 Nov 2006 } 08:10:19 -0600 } 7, 12 -- Mime-Version: 1.0 } 7, 12 -- X-Sender: zaluzec-at-ultra5.microscopy.com (Unverified) 7, 12 -- } Message-Id: {p06110402c1764435990f-at-[206.69.208.22]} } 7, 12 -- Date: Tue, 7 Nov 2006 08:10:18 -0600 } 7, 12 -- To: microscopy-at-microscopy.com } 7, 12 -- From: pavi_micro-at-yahoo.com (by way of Ask-A-Microscopist) 7, } 12 -- Subject: AskAMicroscopist: dark field 7, 12 -- Content-Type: } text/plain; charset="us-ascii" ==============================End of - } Headers============================== } } } } } ==============================Original } Headers============================== } 32, 18 -- From frank.karl-at-degussa.com Tue Nov 7 08:48:20 2006 } 32, 18 -- Received: from malmailout1.rz.itson.com } (mailout1.degussa.com [193.100.56.173]) } 32, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id kA7EmJDj002211 } 32, 18 -- for {microscopy-at-msa.microscopy.com} ; Tue, 7 Nov 2006 } 08:48:20 -0600 } 32, 18 -- Received: from mobuscomm01.mail.degussa.com } (uss1026.applications.degussanet.com [10.88.88.98]) } 32, 18 -- by malmailout1.rz.itson.com } (8.13.4/8.13.4/Debian-3sarge3) with ESMTP id kA7EmH0A022231; } 32, 18 -- Tue, 7 Nov 2006 15:48:17 +0100 } 32, 18 -- In-Reply-To: {200611071412.kA7EC5LG007708-at-ns.microscopy.com} } 32, 18 -- Subject: Re: [Microscopy] AskAMicroscopist: dark field } 32, 18 -- To: pavi_micro-at-yahoo.com, microscopy-at-msa.microscopy.com } 32, 18 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 } 32, 18 -- Message-ID: } {OF1AB8A2F0.51F14B6F-ON8625721F.004E2BCB-8625721F.00515177-at-degussa.com} } 32, 18 -- From: frank.karl-at-degussa.com } 32, 18 -- Date: Tue, 7 Nov 2006 08:48:13 -0600 } 32, 18 -- X-MIMETrack: Serialize by Router on } MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at } 32, 18 -- 11/07/2006 08:48:18 AM } 32, 18 -- MIME-Version: 1.0 } 32, 18 -- Content-type: text/plain; charset=US-ASCII } ==============================End of - Headers==============================
(1) Does anyone have information about third-party backscatter electron detectors suitable for scanning Auger microprobes (compatible with ultra high vacuum)?
(2) Does anyone have an opinion about using an electron backscatter diffraction detector as the only backscatter electron detector in a microscope?
Clifford Todd The Dow Chemical Co. CTodd2-at-dow.com
==============================Original Headers============================== 4, 22 -- From CTodd2-at-dow.com Wed Nov 8 08:43:45 2006 4, 22 -- Received: from mail86.messagelabs.com (mail86.messagelabs.com [216.82.244.115]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kA8EhjB0008092 4, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Nov 2006 08:43:45 -0600 4, 22 -- X-VirusChecked: Checked 4, 22 -- X-Env-Sender: CTodd2-at-dow.com 4, 22 -- X-Msg-Ref: server-11.tower-86.messagelabs.com!1162997021!15799095!1 4, 22 -- X-StarScan-Version: 5.5.10.7; banners=-,-,- 4, 22 -- X-Originating-IP: [216.99.65.27] 4, 22 -- Received: (qmail 8643 invoked from network); 8 Nov 2006 14:43:41 -0000 4, 22 -- Received: from mail7.dow.com (HELO mante88.nam.dow.com) (216.99.65.27) 4, 22 -- by server-11.tower-86.messagelabs.com with SMTP; 8 Nov 2006 14:43:41 -0000 4, 22 -- Received: by mante88.nam.dow.com with Internet Mail Service (5.5.2658.3) 4, 22 -- id {W32RYJ9T} ; Wed, 8 Nov 2006 09:43:40 -0500 4, 22 -- Message-ID: {0F0E6B7B4F6AE84CBC5E7AF57057FBA8077B20-at-USMDLMDOWX022.dow.com} 4, 22 -- From: "Todd, Clifford (C)" {CTodd2-at-dow.com} 4, 22 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 4, 22 -- Subject: backscatter electron detectors 4, 22 -- Date: Wed, 8 Nov 2006 09:44:00 -0500 4, 22 -- MIME-Version: 1.0 4, 22 -- X-Mailer: Internet Mail Service (5.5.2658.3) 4, 22 -- Content-Type: text/plain ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlevin-at-forsyth.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mlevin-at-forsyth.org Name: Michael Levin
Organization: Forsyth Institute
Title-Subject: [Filtered] need recommendations for embedding medium
Question: Hello all -
I am a developmental biologisy and need a recommendation, to help me choose the right embedding medium. First and simplest, does anyone have a replacement for JB4 that is less toxic? I need something that is transparent (so that I can orient my embryos during embedding), and that I will cut with a vibratome later to make 20 micron sections. I currently use JB4 to section embryos after wholemount immunohistochemistry or in situ hybridization but I need something that is less toxic. Second, I'm looking for a different medium that meets the following criteria. I need to be able to embed soft tissues (frog and chick embryos) for sectioning via vibratome (10 microns to 60 microns thick). Unlike with the JB4, I want to do immunohistochemistry on these sections after cutting (but do not need electron microscopy). The embedding medium also needs to be
1) non-toxic (so that a fume hood is not needed during embedding) 2) solidifies into a block that can be cut with vibratome, but not so hard that I can't trim it later with a razor blade 3) is transparent, so that I can orient embryos while embedding
Can anyone suggest any kind of embedding mix that matches this description? Please email me at mlevin-at-forsyth.org. Thank you in advance!
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bclarke-at-agarscientific.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bclarke-at-agarscientific.com Name: Bill Clarke
Organization: Agar Scientific Ltd
Title-Subject: [Filtered] Job opportunity
Question: Technical Support Specialist
Agar Scientific is a leading international supplier of consumables, accessories and specialist equipment for all disciplines of microscopy.
The company has a high level of expertise with wide practical experience in specimen preparation and microscopy techniques.
Based in the UK, the primary role is to provide technical support across our diverse product range to our customers and distributors. The successful applicant will also be working closely with our sales and marketing team to develop new business and product lines. Some UK and international travel may be required.
This is a new appointment and the position offers an ideal opportunity for continued development in a small successful company.
Applicants should be of graduate level (or equivalent) in a science or technology related subject and have experience in electron and optical microscopy techniques. They should have a good 'hands on' approach, be confident in front of customers and have good IT and communication skills. The position would suit a scientist or technician who wishes to build on their existing skills in a commercial environment.
Please apply offline to Dr. Lynne Joyce ljoyce-at-agarscientific.com
Actually I already contacted the german distributor (I am in Autria, not Australia - too bad for me ;-)) and they told me that this material was for 110 volts only. Why the Homo sapiens cannot find a worldwide consensus on the current frequency is out of my capacity of comprehension but it is a fact I have to live with. Apparently I have no other choice than to order a portable heat pen and a kit of rechargeable batteries. Thanks to all for the replies and don't hesitate to add more remarks.
Regards,
Stephane
----- Original Message ---- X-from: "Clarkson, Donna R Contr USAMRD/MCMR-UWB-L" {Donna.Clarkson-at-BROOKS.AF.MIL} To: nizets2-at-yahoo.com Sent: Wednesday, November 8, 2006 3:39:00 PM
Dear colleagues,
It is not easy to find a heat pen in this small isolated country named Austria ;-). The only one I found available is around 600 euros, which I find a LITTLE BIT expensive for such an instrument. Someone told me that a soldering iron would do the trick, and they are available at 10 euros in every general store. Has anyone experienced this electrician tool for EM work? Or perhaps someone has an idea where I could find a "true" heat pen at fair price in our old continent?
Regards,
Stephane
==============================Original Headers============================== 7, 19 -- From nizets2-at-yahoo.com Wed Nov 8 01:24:23 2006 7, 19 -- Received: from web37412.mail.mud.yahoo.com (web37412.mail.mud.yahoo.com [209.191.91.144]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kA87ONDc024130 7, 19 -- for {microscopy-at-microscopy.com} ; Wed, 8 Nov 2006 01:24:23 -0600 7, 19 -- Received: (qmail 19776 invoked by uid 60001); 8 Nov 2006 07:24:22 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Con tent-Transfer-Encoding; 7, 19 -- b=CD/Knw0zoRhb0W0iMDibqVsUDkXBXPEl3Qh+txTK/Cr61x4Ncby+KJycSzmyJQDaWSvJLn +8yvk/mjn1mZff9q2+5umgUs4dJKAN8TokIMfik1JsS1gH4s1Nw8fhXB64ufi+djyUMhGvlo AR/hit5BIpsAaQON7mVb6JCiQdQPE= ; 7, 19 -- Message-ID: {20061108072422.19774.qmail-at-web37412.mail.mud.yahoo.com} 7, 19 -- Received: from [80.122.101.102] by web37412.mail.mud.yahoo.com via HTTP; Tue, 07 Nov 2006 23:24:22 PST 7, 19 -- Date: Tue, 7 Nov 2006 23:24:22 -0800 (PST) 7, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 19 -- Subject: Heat pen 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=ascii 7, 19 -- Content-Transfer-Encoding: 8bit 7, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA87ONDc024130 ==============================End of - Headers==============================
==============================Original Headers============================== 27, 19 -- From nizets2-at-yahoo.com Wed Nov 8 09:03:25 2006 27, 19 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 27, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kA8F3OAS028721 27, 19 -- for {microscopy-at-microscopy.com} ; Wed, 8 Nov 2006 09:03:25 -0600 27, 19 -- Received: (qmail 80265 invoked by uid 60001); 8 Nov 2006 15:03:24 -0000 27, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 27, 19 -- s=s1024; d=yahoo.com; 27, 19 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 27, 19 -- b=1t5mfm0CKFqmX5G6AXJVQ/v02pBiIc7EXU+mP6q6NvTygDMA0rOYm3169s56+1ymgAcPSWrbmtvsax5ezwEV1u0gZxFBFDTso0nHo5J3B0Sm0jpSZJLqFIojHvkcWH3PGEzdcNyKU5RFmu9Xlg2abOEp7XpjRKBYFxdFql7DuIo= ; 27, 19 -- Message-ID: {20061108150324.80263.qmail-at-web37405.mail.mud.yahoo.com} 27, 19 -- Received: from [80.122.101.102] by web37405.mail.mud.yahoo.com via HTTP; Wed, 08 Nov 2006 07:03:24 PST 27, 19 -- Date: Wed, 8 Nov 2006 07:03:24 -0800 (PST) 27, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 27, 19 -- Subject: Re: [Microscopy] Heat pen 27, 19 -- To: "Clarkson, Donna R Contr USAMRD/MCMR-UWB-L" {Donna.Clarkson-at-BROOKS.AF.MIL} 27, 19 -- MIME-Version: 1.0 27, 19 -- Content-Type: text/plain; charset=ascii 27, 19 -- Content-Transfer-Encoding: 8bit 27, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA8F3OAS028721 ==============================End of - Headers==============================
EMS in NJ sells a battery powered (wax) heat pen that is fairly cheap and works with thin sections or for wax sealing of boats. Go to http://www.emsdiasum.com. Search for heat pen. Catalog number 72679 $28 USD. Replacement tips: 72679-RT.
One could hook up a 3 volt DC power supply to replace the two AA batteries. I decided not to do that because I got "free" batteries from a storeroom supply and I wanted it to remain portable. It will eat up batteries fast, if used continuously or often.
I had one of those expensive ones with a big filament wire but I used this heat pen because it had a sharp pointed tip. It was small enough for heating thin sections in a diamond knife boat while looking at them through a microtome microscope. EMS shows a photograph of it on their web page.
HTH,
Paul
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==============================Original Headers============================== 7, 25 -- From beaurega-at-westol.com Wed Nov 8 10:03:59 2006 7, 25 -- Received: from smtp-gateway-5.winbeam.com (smtp-gateway-5.winbeam.com [64.84.97.70]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA8G3wdS019515 7, 25 -- for {microscopy-at-microscopy.com} ; Wed, 8 Nov 2006 10:03:58 -0600 7, 25 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 7, 25 -- by smtp-gateway-5.winbeam.com (8.13.2/8.12.8) with SMTP id kA8G0vHg002840 7, 25 -- for {microscopy-at-microscopy.com} ; Wed, 8 Nov 2006 11:00:58 -0500 7, 25 -- Received: (qmail 26781 invoked by uid 89); 8 Nov 2006 16:00:57 -0000 7, 25 -- Received: from pitts-69-72-21-212.dynamic-dialup.coretel.net (HELO millenium) (69.72.21.212) 7, 25 -- by mail.winbeam.com with SMTP; 8 Nov 2006 16:00:57 -0000 7, 25 -- Message-Id: {3.0.6.32.20061108110121.007f3410-at-pop3.norton.antivirus} 7, 25 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus 7, 25 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 7, 25 -- Date: Wed, 08 Nov 2006 11:01:21 -0500 7, 25 -- To: nizets2-at-yahoo.com, microscopy-at-microscopy.com 7, 25 -- From: Beaurega {beaurega-at-westol.com} 7, 25 -- Subject: Re: [Microscopy] Heat pen 7, 25 -- In-Reply-To: {200611080725.kA87P1Gw024912-at-ns.microscopy.com} 7, 25 -- Mime-Version: 1.0 7, 25 -- Content-Type: text/plain; charset="us-ascii" 7, 25 -- X-Winbeam-MailScanner-Information: smtp-gateway-5.winbeam.com - Please contact Technical Support for more information 7, 25 -- X-Winbeam-MailScanner: Found to be clean [smtp-gateway-5.winbeam.com] (courtesy of Winbeam) 7, 25 -- X-Winbeam-MailScanner-SpamCheck: not spam, SpamAssassin (cached, 7, 25 -- score=-2.599, required 4, BAYES_00 -2.60) 7, 25 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com ==============================End of - Headers==============================
EMS sells many fine products including this one but as an owner of this battery powered pen and a 115 V heat pen from Ted Pella, I find there is no comparison for the purpose of expanding sections. the 115 V pen is significantly better (and significantly more expensive). For wax sealing, the battery pen may be sufficient but I use nail polish so can't directly address that.
At 10:05 AM 11/08/06, you wrote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
The use of a central stop in the objective will decrease resolution.
The use of a stop either in the condenser or at the light source (strong light source) allows better resolution (I've tested this; Dodge also mentions a bit of this in 1948). Ted asked me to publish an article on our paper but after looking at numerous articles over the years, I thought it was rehashing history unless I apply it in more detail to sometime relevant (asbestos or pharmaceutical contaminants). Perhaps I'll finish it off in another year or two.
I believe the improvement is because if the high NA at the critical point, combined with uniformity of background (contrast to phase chnges).
Tony
.......................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC PO Box 34140 Indianapolis, IN 46234 (317) 752-6386 (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%â„
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-----Original Message----- X-from: frank.karl-at-degussa.com [mailto:frank.karl-at-degussa.com] Sent: Wednesday, November 08, 2006 6:58 AM To: ph2-at-sprynet.com; microscopy-at-msa.microscopy.com
A few more Comments on Darkfield - but I like the contrast comparison.
1. Part I - Darkfield history
Darkfield has been around a long time (1800s), including paraboloid and ellipsoid condenser uses.
Classically done with a stop in the condenser or with a reflecting objective.
But can be done with a stop on the objective, at the light source, or another focal plane before the eye.
Modifications of this are:
A) with dispersion staining [promoted by Crossman as well as Brown and McCrone in the 1960s, but actually first described by Dodge in Am. Min. in 1948]
or
B) critical darkfield (you can look up an article by Ted Clarke in Microscopy Today on critical Darkfield which comes from: Havics & Clarke: “Critical Darkfield and Its Application to Asbestos Analysis” presented at Inter/Micro-2002, June 24-27, 2002, Chicago, IL.; there are some nice diagrams of the process of light interaction)
2. Part II - Contrast Issues
Phase
I(x’) proportional to [1 - (2*(phase dif)(x’)) ] I = intensity Thus linear with phase change (RI*thickness) useful for up to 1/2 wavelength Best at {1/10 wavelength
Darkfield
I(x’) proportional to (phase dif)^2 (ingoring secondary terms) Thus not so linear with phase change And useful for outlines or very small particles/differences
Hoffman Modulation Contrast
dI = If*(Phase dif)*(TB - TG)*2cos(theta/w) TB = transmittance in bright area TG = transmittance in gray area W = width of slit Theta = angle from perpedicular to slit Thus non-linear but smoothed
Normaski DIC
I proportional to d(dif phase)/dx (simplified) useful from } 1/10 wavelength Up to 1 wavelength best above 1/2 wavelength
[Source for above: Havics: “Asbestos Fiber Counting by Different Optical Contrast Techniques”, presented at Inter/Micro-2003, July 7-10, 2003, Chicago, IL.; I'm trying to get an article out on this in the next 3 months or so which is why it's at my fingertips]
3. Part III additional Comments
Resolution of Darkfield} Hoffman} Phase } DIC } Brightfield based on my tests using a resolution test slide (HSE/NPL test slide). Fluorescence is too media dependent to make a broad statement but does better than Hoffman using fluorescent spheres and about the same as darkfield.
Resolution does not necessarily mean detectability. Contrast is better for detectability of an object whereas resolution is better for defining the structure once detected.
A Dark background in general increases detectability vs a bright background.
Tony
.......................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC PO Box 34140 Indianapolis, IN 46234 (317) 752-6386 (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%â„
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-----Original Message----- X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] Sent: Tuesday, November 07, 2006 9:55 AM To: ph2-at-sprynet.com
Microscopists, While Frank Karl is undoubtedly correct that the spur to invent dark field was based on resolution and tilting beams, I wanted to mention that there is a useful rubric for classifying types of microscopy, and that is based on contrast. Like this:
In each case, the microscope takes advantage of a specific kind of interaction between light and matter to generate contrast (of course there are overlaps, eg scattering contributes to contrast in a brightfield microscope, etc).
(The Nomarski one may be hard to understand, this kind of optics gives rise to a signal whenever the gradient in optical path in a local region is different than what the gradient is in the background).
Hope this helps, Tobias
} } } What an interesting question. } } A Dark field scope produces an image of brightly illuminated objects } on a dark background. It was one of the earliest attempts to increase } the resolution and detectability of objects. } } Abbe's theory of resolution states that for good resolution you need to } capture two of three possible rays. This is assuming that each } microscopic object acts as a diffraction grating. This isn't too bad } of an assumption. The three rays are one direct, undeviated ray from } the sample and two primary diffracted rays. (We like to think of these } thought experiment and ray diagrams has two dimensional drawing on a } sheet of paper). For some subjects the diffracted rays fall outside of } the light accepting ability of the objective and these objects can not } be resolved. (Bummer!) } } One approach is to tilt or move the condense off center (Many of the } old medical grade scopes had this ability). This allows the undeviated } ray and one diffracted ray to enter the front lens of the objective. } This produces a dark background and white illuminated subjects. } } A second approach is to use a central stop in the condenser to block } the central ray and allow only highly angled rays to illuminated the } subject. The diffracted rays from these angled light rays form the } image. } } You often need a powerful light source or work in a very dark room with } dark adapted eyes because you are throwing away a lot of light with a } central stop. Special condensers were, and I believe, are still made } to efficiently produce darkfield illumination. } } Newer illuminating systems (like phase contrast) have replaced a lot of } darkfield work. Still some incredible images can be formed with } darkfield. One easy short cut is to use a phase contrast scope and } select a condenser phase ring larger than the ring in your objective. } } Best wishes........... } } Frank Karl } Degussa Coproration } } } } }
} } pavi_micro-at-yahoo.
} com To: } frank.karl-at-degussa.com } } cc:
} 11/07/2006 08:12 Subject: } [Microscopy] AskAMicroscopist: dark } field } } AM
} Please respond } to
} } pavi_micro
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} } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (pavi_micro-at-yahoo.com) from } http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on } Monday, November 6, 2006 at 23:57:52 } ----------------------------------------------------------------------- } ---- } } Email: pavi_micro-at-yahoo.com } Name: mrs.pavitra jain } } Organization: Microbiologist } } Education: Graduate College } } Location: Hubli,India } } Question: What is dark field microscope } } ----------------------------------------------------------------------- } ---- } } ==============================Original } Headers============================== } 7, 12 -- From zaluzec-at-ultra5.microscopy.com Tue Nov 7 08:10:19 2006 7, } 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id kA7EAJO5003959 } 7, 12 -- for {microscopy-at-microscopy.com} ; Tue, 7 Nov 2006 } 08:10:19 -0600 } 7, 12 -- Mime-Version: 1.0 } 7, 12 -- X-Sender: zaluzec-at-ultra5.microscopy.com (Unverified) 7, 12 -- } Message-Id: {p06110402c1764435990f-at-[206.69.208.22]} } 7, 12 -- Date: Tue, 7 Nov 2006 08:10:18 -0600 } 7, 12 -- To: microscopy-at-microscopy.com } 7, 12 -- From: pavi_micro-at-yahoo.com (by way of Ask-A-Microscopist) 7, } 12 -- Subject: AskAMicroscopist: dark field 7, 12 -- Content-Type: } text/plain; charset="us-ascii" ==============================End of - } Headers============================== } } } } } ==============================Original } Headers============================== } 32, 18 -- From frank.karl-at-degussa.com Tue Nov 7 08:48:20 2006 32, 18 } -- Received: from malmailout1.rz.itson.com (mailout1.degussa.com } [193.100.56.173]) } 32, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id kA7EmJDj002211 } 32, 18 -- for {microscopy-at-msa.microscopy.com} ; Tue, 7 Nov 2006 } 08:48:20 -0600 } 32, 18 -- Received: from mobuscomm01.mail.degussa.com } (uss1026.applications.degussanet.com [10.88.88.98]) } 32, 18 -- by malmailout1.rz.itson.com } (8.13.4/8.13.4/Debian-3sarge3) with ESMTP id kA7EmH0A022231; } 32, 18 -- Tue, 7 Nov 2006 15:48:17 +0100 } 32, 18 -- In-Reply-To: {200611071412.kA7EC5LG007708-at-ns.microscopy.com} } 32, 18 -- Subject: Re: [Microscopy] AskAMicroscopist: dark field 32, 18 } -- To: pavi_micro-at-yahoo.com, microscopy-at-msa.microscopy.com 32, 18 -- } X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 32, 18 -- } Message-ID: } {OF1AB8A2F0.51F14B6F-ON8625721F.004E2BCB-8625721F.00515177-at-degussa.com} } 32, 18 -- From: frank.karl-at-degussa.com } 32, 18 -- Date: Tue, 7 Nov 2006 08:48:13 -0600 } 32, 18 -- X-MIMETrack: Serialize by Router on } MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 32, 18 -- } 11/07/2006 08:48:18 AM 32, 18 -- MIME-Version: 1.0 } 32, 18 -- Content-type: text/plain; charset=US-ASCII } ==============================End of - Headers==============================
(1) KE and Robinson make scintillator BSD units. The key I think for ultra high vacuum is the use of bellows rather than O-ring seals. Robinson makes this. Not sure about KE. The other option is a solid state BSD which just needs a feed through port for the diode signal wires. But performance is not as good as with scintillator units.
(2) Do you mean EBSD? If so, then the answer is no. EBSD is totally different from BSD. BSD is elemental contrast while EBSD is crystal lattice info. However, using EDS together with EBSD can produce very useful results for multi-phase specimens, if applicable. Note that EBSD does not work on amorphous specimens or areas.
gary g.
At 06:45 AM 11/8/2006, you wrote:
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==============================Original Headers============================== 9, 20 -- From gary-at-gaugler.com Wed Nov 8 10:45:30 2006 9, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kA8GjTa1019416 9, 20 -- for {microscopy-at-microscopy.com} ; Wed, 8 Nov 2006 10:45:29 -0600 9, 20 -- Received: (qmail 13091 invoked from network); 8 Nov 2006 08:45:29 -0800 9, 20 -- Received: by simscan 1.1.0 ppid: 13075, pid: 13088, t: 0.1712s 9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 9, 20 -- by qsmtp3 with SMTP; 8 Nov 2006 08:45:29 -0800 9, 20 -- Message-Id: {7.0.1.0.2.20061108083853.024c7570-at-gaugler.com} 9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 9, 20 -- Date: Wed, 08 Nov 2006 08:45:28 -0800 9, 20 -- To: CTodd2-at-dow.com 9, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 20 -- Subject: Re: [Microscopy] backscatter electron detectors 9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 20 -- In-Reply-To: {200611081445.kA8EjlZI011023-at-ns.microscopy.com} 9, 20 -- References: {200611081445.kA8EjlZI011023-at-ns.microscopy.com} 9, 20 -- Mime-Version: 1.0 9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I don't have a suggestion for a less toxic replacement for JB4 but agar may do the trick for your second embedding proposition. You could try embedding in 3% low gel point agar which liquifies fairly quickly in a waterbath at 60'C, stays liquid to about 35'C and is quite solid at RT. If necesary (and if it doesn't compromise your subsequent procedures), you can probably get better support / better sections by immersing in fixative (aldehyde or ethanol) to make the gel more solid.
Hope this helps,
Alastair At 09:06 08/11/2006 -0600, you wrote:
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Alastair McKinnon Histology & EM Facility Manager University of Aberdeen, Institute of Medical Science Foresterhill, Aberdeen, AB25 2ZD tel: +44(0) 1224 552923 (office); +44(0) 1224 555911 (lab) fax: +44(0) 1224 559533; email: a.d.mckinnon-at-abdn.ac.uk www.abdn.ac.uk/ims/h-em
==============================Original Headers============================== 10, 19 -- From a.d.mckinnon-at-abdn.ac.uk Wed Nov 8 11:38:32 2006 10, 19 -- Received: from mailhub3.abdn.ac.uk (mailhub3.abdn.ac.uk [139.133.7.13]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA8HcVRV031466 10, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Nov 2006 11:38:31 -0600 10, 19 -- Received: from med-0069.ims.abdn.ac.uk ([139.133.159.90] helo=med-0069.abdn.ac.uk) 10, 19 -- by mailhub3.abdn.ac.uk with esmtp (Exim 4.52) 10, 19 -- id 1GhrNV-0003dq-BE; Wed, 08 Nov 2006 17:38:21 +0000 10, 19 -- Message-Id: {5.2.1.1.0.20061108172609.011101d0-at-mailms.abdn.ac.uk} 10, 19 -- X-Sender: pat081-at-mailms.abdn.ac.uk 10, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 10, 19 -- Date: Wed, 08 Nov 2006 17:38:18 +0000 10, 19 -- To: mlevin-at-forsyth.org 10, 19 -- From: Alastair McKinnon {a.d.mckinnon-at-abdn.ac.uk} 10, 19 -- Subject: Re: [Microscopy] viaWWW: need recommendations for embedding 10, 19 -- medium 10, 19 -- Cc: Microscopy-at-microscopy.com 10, 19 -- In-Reply-To: {200611081506.kA8F6KGX005773-at-ns.microscopy.com} 10, 19 -- Mime-Version: 1.0 10, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Look around for a conversion transformer. These convert between 120 and 240 and vice versa. You would need a unit that would handle the VA or Watts or Amps that the pen requires.
I got one of these at our US Radio Shack store to convert 120 to 240 for an Olympus PM-10AD photo controller. It cost about $24US.
gary g.
At 07:06 AM 11/8/2006, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Wed Nov 8 11:58:58 2006 10, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kA8Hwv1i009955 10, 20 -- for {microscopy-at-microscopy.com} ; Wed, 8 Nov 2006 11:58:57 -0600 10, 20 -- Received: (qmail 26489 invoked from network); 8 Nov 2006 09:58:52 -0800 10, 20 -- Received: by simscan 1.1.0 ppid: 26411, pid: 26487, t: 0.2096s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp1 with SMTP; 8 Nov 2006 09:58:52 -0800 10, 20 -- Message-Id: {7.0.1.0.2.20061108095545.024e4118-at-gaugler.com} 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 10, 20 -- Date: Wed, 08 Nov 2006 09:58:51 -0800 10, 20 -- To: nizets2-at-yahoo.com 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] Re: Heat pen 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200611081506.kA8F63cG004626-at-ns.microscopy.com} 10, 20 -- References: {200611081506.kA8F63cG004626-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
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Title-Subject: [Filtered] Help with settings for Canon EOS D60 on Leica DM LB2
Question: I was wondering if someone would be able to help me sort out the settings for a microscope-camera set up I am trying to use to take images of diatoms.
The set up we have is a Leica DM LB2 High Powered Light Microscope with a Leica C Plan 100x1.25 Oil Magnifying Lens. On top is attachment EF-bayonet Leica HC 1.6x for Canon D-SLR Camera. We then have a Canon EOS D60 camera ontop (its quite an old one as the digital out is not USB!). We have been using the camera on AV as suggested in the bayonet instructions. However, what ever changes we make we cant seam to get the quality of image one would expect.
Does anyone have any ideas of what is needing too be changed?
My BSE system was made by a third party manufacturer, GW Electronics. GW disbanded and was sold to: http://www.kedev.co.uk/
I have not yet purchased anything from these folks, but perhaps they could help...
Woody White BWXT Services
-----Original Message----- X-from: CTodd2-at-dow.com [mailto:CTodd2-at-dow.com] Sent: Wednesday, November 08, 2006 9:44 AM To: White, Woody N.
(1) Does anyone have information about third-party backscatter electron detectors suitable for scanning Auger microprobes (compatible with ultra high vacuum)?
(2) Does anyone have an opinion about using an electron backscatter diffraction detector as the only backscatter electron detector in a microscope?
Clifford Todd The Dow Chemical Co. CTodd2-at-dow.com
==============================Original Headers============================== 4, 22 -- From CTodd2-at-dow.com Wed Nov 8 08:43:45 2006 4, 22 -- Received: from mail86.messagelabs.com (mail86.messagelabs.com [216.82.244.115]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kA8EhjB0008092 4, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Nov 2006 08:43:45 -0600 4, 22 -- X-VirusChecked: Checked 4, 22 -- X-Env-Sender: CTodd2-at-dow.com 4, 22 -- X-Msg-Ref: server-11.tower-86.messagelabs.com!1162997021!15799095!1 4, 22 -- X-StarScan-Version: 5.5.10.7; banners=-,-,- 4, 22 -- X-Originating-IP: [216.99.65.27] 4, 22 -- Received: (qmail 8643 invoked from network); 8 Nov 2006 14:43:41 -0000 4, 22 -- Received: from mail7.dow.com (HELO mante88.nam.dow.com) (216.99.65.27) 4, 22 -- by server-11.tower-86.messagelabs.com with SMTP; 8 Nov 2006 14:43:41 -0000 4, 22 -- Received: by mante88.nam.dow.com with Internet Mail Service (5.5.2658.3) 4, 22 -- id {W32RYJ9T} ; Wed, 8 Nov 2006 09:43:40 -0500 4, 22 -- Message-ID: {0F0E6B7B4F6AE84CBC5E7AF57057FBA8077B20-at-USMDLMDOWX022.dow.com} 4, 22 -- From: "Todd, Clifford (C)" {CTodd2-at-dow.com} 4, 22 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 4, 22 -- Subject: backscatter electron detectors 4, 22 -- Date: Wed, 8 Nov 2006 09:44:00 -0500 4, 22 -- MIME-Version: 1.0 4, 22 -- X-Mailer: Internet Mail Service (5.5.2658.3) 4, 22 -- Content-Type: text/plain ==============================End of - Headers==============================
==============================Original Headers============================== 17, 26 -- From nwwhite-at-bwxt.com Wed Nov 8 14:54:20 2006 17, 26 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA8KsIW7002395 17, 26 -- for {microscopy-at-msa.microscopy.com} ; Wed, 8 Nov 2006 14:54:20 -0600 17, 26 -- Received: from ([131.184.13.224]) 17, 26 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.2734684; 17, 26 -- Wed, 08 Nov 2006 15:37:49 -0500 17, 26 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.30]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); 17, 26 -- Wed, 8 Nov 2006 14:50:13 -0500 17, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 26 -- Content-class: urn:content-classes:message 17, 26 -- MIME-Version: 1.0 17, 26 -- Content-Type: text/plain; 17, 26 -- charset="us-ascii" 17, 26 -- Subject: RE: [Microscopy] backscatter electron detectors 17, 26 -- Date: Wed, 8 Nov 2006 13:49:32 -0500 17, 26 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB703D0D1-at-BWXSPO01.BWXS.BWXTECH.NET} 17, 26 -- X-MS-Has-Attach: 17, 26 -- X-MS-TNEF-Correlator: 17, 26 -- Thread-Topic: [Microscopy] backscatter electron detectors 17, 26 -- Thread-Index: AccDSQZuytlkuKLYTp2l93z27hSmnwAHSoIg 17, 26 -- From: "White, Woody N." {NWWhite-at-bwxt.com} 17, 26 -- To: {CTodd2-at-dow.com} , "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 17, 26 -- X-OriginalArrivalTime: 08 Nov 2006 19:50:13.0320 (UTC) FILETIME=[1B095C80:01C7036F] 17, 26 -- Content-Transfer-Encoding: 8bit 17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA8KsIW7002395 ==============================End of - Headers==============================
Mike A company called London Resin produce a series of acrylic resins including LR White. The resin is suitable for LM sections up to 15-20um or em sections, can be used in a lot of immuno labelling. It is transparent and has low toxicity (you may need to check if that's lower than JB4).
In the UK it is marketed by Agar Scientific Ltd, 66a Cambridge Road, Stansted, Essex, CM24 8DA, UK (Tel 0279 813519). But I'm sure that there will be a US outlet (if that's where you are).
The downside is: 1. it may be more expensive than JB4 2. it needs to be polymerised in the absence of air (oxygen). For e.m we use gelatine capsules filled up and a coverslip or lid on each one to exclude air. 3. it is recommended that thinner sections are cut on glass Ralph knives, but thick ones may not need this (I don't know).
I hope this helps.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: mlevin-at-forsyth.org
I know that it could seem very little professional... but I get really good pictures just positioning the digital camera on the ocular of the microscope. You just need a good screen on the camera to control focusing and maybe take more than one picture and choose later the best. I bypassed all problems on attachment and bayonets... I insert measure scale in pictures with ImageJ from a picture of a graduated slide. I am dealing mostly with mammal hair samples, but I tried just for fun with other kind of samples with the same good results
bye!
antonella ______________________________________ Antonella Stravisi Dipartimento di Scienze Animali Universitŕ di Udine via S. Mauro, 2 33010 Pagnacco (UD) Italy
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Email: dfine-at-seton.org Name: Diann Fine
Organization: Brackenridge Hospital
Education: Graduate College
Location: Austin, Texas, USA
Title: Leica EMPT
Question: I would appreciate some information from the EM labs using the Leica EMTP. Is it user-friendly and reliable? Would you purchase it again? Were there any problems or issues with it? Do you run 2 different processing programs together at the same time? Do you run separate procedures for kidney and muscle biopsies? Are different procedures run for each different tissue? Thanks for the replies.
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Email: mkelly-at-fs.fed.us Name: M
Organization: USDA
Title-Subject: [Filtered] LRWhite
Question: I have been using LR White resin for immunolabeling. I have problems with contrast in my samples. Sometimes several sections will be too poor a contrast to get pictures and sometimes part of a section will be just adequate contrast. I understand too long an infiltration time can give you contrast problems. I've cut this down but can only cut it back so far as my material is difficult to get embeded.I would sure appreciate any ideas . Thanks, M
Clifford Todd wrote: =============================================== (1) Does anyone have information about third-party backscatter electron detectors suitable for scanning Auger microprobes (compatible with ultra high vacuum)?
(2) Does anyone have an opinion about using an electron backscatter diffraction detector as the only backscatter electron detector in a microscope?
========================================= To answer the questions:
(1) The Robinson detector is not bakeable, but has been installed and successfully operated in microscopes with chamber vacuums in the vicinity of 10exp-8 torr. I am unaware of other BSE detectors that will operate at lower pressures. The detector is retractable and if you wish to have a bellows retraction mechanism, that is also available. See URL http://www.2spi.com/catalog/instruments/robinson-backscattered-electron-BSE-detector.shtml for further information.
(2) As has been pointed out, EBSD requires a different detector from a BSE detector. But I believe one could operate them simultaneously by the appropriate choice of detectors - a BSE detector above the specimen and an EBSD to one side of the specimen.
Disclaimer: SPI Supplies is the North American distributor for the line of Robinson BSE detectors so we are not exactly a disinterested third party.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
==============================Original Headers============================== 11, 25 -- From cgarber-at-2spi.com Thu Nov 9 08:56:19 2006 11, 25 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA9EuGI5013154 11, 25 -- for {microscopy-at-msa.microscopy.com} ; Thu, 9 Nov 2006 08:56:17 -0600 11, 25 -- Received: from webmail.idv.net (diskless6.external [209.120.196.50]) 11, 25 -- (authenticated bits=0) 11, 25 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id kA9EuCc1019000 11, 25 -- for {microscopy-at-msa.microscopy.com} ; Thu, 9 Nov 2006 09:56:16 -0500 11, 25 -- X-IDV-FirstRcvd: diskless6.external [209.120.196.50] 11, 25 -- X-IDV-HELO: webmail.idv.net 11, 25 -- X-IDV-Authenticated-User: cgarber 11, 25 -- Received: from 71.230.36.118 (auth. user cgarber-at-mail.2spi.com) 11, 25 -- by webmail.idv.net with HTTP; Thu, 09 Nov 2006 09:56:12 -0500 11, 25 -- To: microscopy-at-msa.microscopy.com 11, 25 -- Subject: BSE and EBSD detectors 11, 25 -- Date: Thu, 09 Nov 2006 09:56:12 -0500 11, 25 -- X-Mailer: IlohaMail/0.9.0 (On: webmail.idv.net) 11, 25 -- Message-ID: {a6Qga3Mz.1163084172.3252680.cgarber-at-mail.2spi.com} 11, 25 -- From: {cgarber-at-2spi.com} 11, 25 -- Bounce-To: {cgarber-at-2spi.com} 11, 25 -- Errors-To: {cgarber-at-2spi.com} 11, 25 -- MIME-Version: 1.0 11, 25 -- Content-Type: text/plain; charset=ISO-8859-1 11, 25 -- Content-Transfer-Encoding: 8bit 11, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kA9EuGI5013154 ==============================End of - Headers==============================
One way to add contrast without affecting antigenicity too much is to add ~0.2-2% tannic acid to fix or freeze-sub solvent, followed by a similar concentration of ferric chloride (rather than OsO4). How strong a concentration you use and for how long varies quite a bit with tissue, and I've only ever processed plant tissues - which often need reasonably long (2 days to a few weeks) infiltrations. I've also played around with the U acetate stain - 2% UA in 70% ethanol for not too long (2-5 min) worked reasonably well on thickish (dark gold) sections of medium grade LRWhite.
good luck,
Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 61 (0)2-6246 5475 GPO Box 1600 mob. 61 (0)402 835 973 Canberra, ACT 2601 fax. 61 (0)2-6246 5334 Australia
} From: mkelly-at-fs.fed.us } Reply-To: mkelly-at-fs.fed.us } Date: Thu, 9 Nov 2006 08:07:39 -0600 } To: rosemary.white-at-csiro.au } Subject: [Microscopy] viaWWW: LRWhite } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mkelly-at-fs.fed.us as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mkelly-at-fs.fed.us } Name: M } } Organization: USDA } } Title-Subject: [Filtered] LRWhite } } Question: I have been using LR White resin for immunolabeling. I have problems } with contrast in my samples. Sometimes several sections will be too poor a } contrast to get pictures and sometimes part of a section will be just adequate } contrast. I understand too long an infiltration time can give you contrast } problems. I've cut this down but can only cut it back so far as my material is } difficult to get embeded.I would sure appreciate any ideas . } Thanks, M } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 12 -- From zaluzec-at-microscopy.com Thu Nov 9 08:05:58 2006 } 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } kA9E5ulj001109 } 6, 12 -- for {microscopy-at-microscopy.com} ; Thu, 9 Nov 2006 08:05:57 -0600 } 6, 12 -- Mime-Version: 1.0 } 6, 12 -- X-Sender: (Unverified) } 6, 12 -- Message-Id: {p06110402c178e634940d-at-[206.69.208.22]} } 6, 12 -- Date: Thu, 9 Nov 2006 08:05:54 -0600 } 6, 12 -- To: microscopy-at-microscopy.com } 6, 12 -- From: mkelly-at-fs.fed.us (by way of MicroscopyListserver) } 6, 12 -- Subject: viaWWW: LRWhite } 6, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 23 -- From Rosemary.White-at-csiro.au Thu Nov 9 15:12:49 2006 5, 23 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au [150.229.7.37]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA9LCltm010426 5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 9 Nov 2006 15:12:48 -0600 5, 23 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=q0z5RimATmBXKJ7R1xHLFTOLggixmsPKlUOlrygPDY8OdeS+RoEhw7a+ojjYDF7CDtBZWZOntw44g44f1aObmG8G66+q0jFy1OjIOkgUXZGoAVQxiPVv6Sg8cWhOLKxu; 5, 23 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 5, 23 -- by act-MTAout1.csiro.au with ESMTP; 10 Nov 2006 08:12:46 +1100 5, 23 -- X-IronPort-AV: i="4.09,407,1157292000"; 5, 23 -- d="scan'208"; a="121760345:sNHT2960538686" 5, 23 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 5, 23 -- Fri, 10 Nov 2006 08:11:12 +1100 5, 23 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 5, 23 -- Date: Fri, 10 Nov 2006 08:13:46 +1100 5, 23 -- Subject: Re: [Microscopy] viaWWW: LRWhite 5, 23 -- From: Rosemary White {Rosemary.White-at-csiro.au} 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- CC: {mkelly-at-fs.fed.us} 5, 23 -- Message-ID: {C179E53A.1A2C0%Rosemary.White-at-csiro.au} 5, 23 -- In-Reply-To: {200611091407.kA9E7dih005553-at-ns.microscopy.com} 5, 23 -- Mime-version: 1.0 5, 23 -- Content-type: text/plain; charset="US-ASCII" 5, 23 -- Content-transfer-encoding: 7bit 5, 23 -- X-OriginalArrivalTime: 09 Nov 2006 21:11:13.0005 (UTC) FILETIME=[960C31D0:01C70443] ==============================End of - Headers==============================
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Email: mkelly-at-fs.fed.us Name: mkelly
Organization: USDA
Title-Subject: [Filtered] LR White
Question: In my earlier post I left out that I am using a TEM for my imunolabeling work. Thanks again for any help anyone can give.
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Email: martini-at-accurel.com Name: Martin Izquierdo
Organization: Accurel
Title-Subject: [Filtered] DualBeam Operator job
Question: Accurel Systems is currently looking for experienced Dual Beam operators. Accurel is located in Sunnyvale, CA
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Email: swtkeller-at-yahoo.com Name: Sandra Keller
Organization: TEM Lab
Title-Subject: [Filtered] TEM-Looking for a general shallow junction etch recipe for silicon
Question: Hi All: I am looking for a general shallow junction etch/stain recipe and procedure for use with silicon for use in TEM shallow junction delineation. Thanks for being such an important resource. Sandra Keller TEM Lab
How is temperature an issue in scanning Auger microprobes. I'm not familiar with these. If heat is an issue, then I agree that heat is not good for scintillator detectors since the detector tip is basically plastic. However, the current Robinsons are metal coated. But I doubt it is sufficient for very hot environments.
BSD for EBSD basically does not work. The killer is the high tilt angle (70 degrees) of the specimen. What does work is fitting the EBSD camera phosphor screen with a Forward Scatter Detector (FSD). The FSD works just like a BSD but at the high tilt. With properly prepared specimens, FSD images are like 3-D. Very good for estimating ROI and step size.
gary g.
At 06:58 AM 11/9/2006, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Thu Nov 9 15:53:13 2006 11, 20 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kA9LrD21021095 11, 20 -- for {microscopy-at-microscopy.com} ; Thu, 9 Nov 2006 15:53:13 -0600 11, 20 -- Received: (qmail 3044 invoked from network); 9 Nov 2006 13:53:12 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 3016, pid: 3042, t: 0.1728s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 20 -- by qsmtp2 with SMTP; 9 Nov 2006 13:53:12 -0800 11, 20 -- Message-Id: {7.0.1.0.2.20061109133741.025c7e70-at-gaugler.com} 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 11, 20 -- Date: Thu, 09 Nov 2006 13:53:07 -0800 11, 20 -- To: cgarber-at-2spi.com 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] BSE and EBSD detectors 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200611091458.kA9Ew8jc016105-at-ns.microscopy.com} 11, 20 -- References: {200611091458.kA9Ew8jc016105-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Gary Gaugler wrote: ================================================ } Chuck: } } How is temperature an issue in scanning Auger microprobes. } I'm not familiar with these. If heat is an issue, then } I agree that heat is not good for scintillator detectors } since the detector tip is basically plastic. However, the } current Robinsons are metal coated. But I doubt it is } sufficient for very hot environments. } } BSD for EBSD basically does not work. The killer is the } high tilt angle (70 degrees) of the specimen. What does } work is fitting the EBSD camera phosphor screen with a } Forward Scatter Detector (FSD). The FSD works just like } a BSD but at the high tilt. With properly prepared } specimens, FSD images are like 3-D. Very good for estimating } ROI and step size. ================================================== It has been my understanding that temperature can at times be important in an Auger system because the energy of Auger electrons is low and the Auger electrons come from very close to the surface, perhaps the top nm or so. If the surface is contaminated with a gas, or some other surface-sorbed species, the Auger electrons, which are related to the material composition of the surface, would only generate a signal from the contamination layer, not the material underneath that it was intended to study. The conventinal wisdom is that UHV and bake out assure low contamination at the surface to be analyzed.
Auger electrons also have a typical energy of 1 - 2 kV and as such are best excited by lower energy electron beams, usually less than 5 kV, and a BSE detector that responds well to low kV electrons is required for BSE imaging at those voltages.
None of the Robinson designed detectors, including the latest versions is good for heating and bakeout. I am reasonably certain this would be the case for any other BSE detector but of course, I could be wrong about that. For the Robinson BSE detectors, the maximum temperature they will withstand is slightly under 50 C.
I presume Dr. Robinson might be following this conversation and perhaps he can explain this better.
Disclaimer: My firm, SPI Supplies is the North American distributor for the Robinson BSE detector product line so we are not a disinterested third party.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
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==============================Original Headers============================== 11, 27 -- From cgarber-at-2spi.com Fri Nov 10 00:43:07 2006 11, 27 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAA6h6PR032238 11, 27 -- for {microscopy-at-msa.microscopy.com} ; Fri, 10 Nov 2006 00:43:07 -0600 11, 27 -- Received: from yourb27fb1c401 (c-71-230-36-118.hsd1.pa.comcast.net [71.230.36.118]) 11, 27 -- (authenticated bits=0) 11, 27 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id kAA6biNP016895; 11, 27 -- Fri, 10 Nov 2006 01:37:51 -0500 11, 27 -- X-IDV-FirstRcvd: c-71-230-36-118.hsd1.pa.comcast.net [71.230.36.118] 11, 27 -- X-IDV-HELO: yourb27fb1c401 11, 27 -- X-IDV-Authenticated-User: cgarber 11, 27 -- Message-ID: {00ff01c70492$bb974da0$6401a8c0-at-yourb27fb1c401} 11, 27 -- From: "Garber, Charles A" {cgarber-at-2spi.com} 11, 27 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 11, 27 -- References: {200611092155.kA9LtFHu023763-at-ns.microscopy.com} 11, 27 -- Subject: BSE and EBSD detectors 11, 27 -- Date: Fri, 10 Nov 2006 01:37:39 -0500 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- format=flowed; 11, 27 -- charset="iso-8859-1"; 11, 27 -- reply-type=original 11, 27 -- Content-Transfer-Encoding: 7bit 11, 27 -- X-Priority: 3 11, 27 -- X-MSMail-Priority: Normal 11, 27 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2869 11, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 ==============================End of - Headers==============================
Hi Sandra, As far as I'm aware, the only junction delineation stain that has been used successfully for TEM work is 0.5% HF in Nitric. This has been a well- established technique for looking at junctions in Si for years. A fairly recent example for shallow junctions is given in Yoo et al., Appl. Phys. Letts. 80, 2687 (2002). You do have to take some precautions to get a good result. For example, if your sample is mounted on a Cu grid, you have to protect this from the nitric acid by coating it with lacquer or something similar. I seem to remember Ron Anderson telling me he used to cool the etchant well below room temperature to slow the etch rate down and make it easier to control. HF is a particularly nasty acid, so if you have no experience of it make sure you are properly trained in its use before you start.
-----Original Message----- X-from: swtkeller-at-yahoo.com [mailto:swtkeller-at-yahoo.com] Sent: 09 November 2006 21:23 To: Richard Beanland
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Email: swtkeller-at-yahoo.com Name: Sandra Keller
Organization: TEM Lab
Title-Subject: [Filtered] TEM-Looking for a general shallow junction etch recipe for silicon
Question: Hi All: I am looking for a general shallow junction etch/stain recipe and procedure for use with silicon for use in TEM shallow junction delineation. Thanks for being such an important resource. Sandra Keller TEM Lab
==============================Original Headers============================== 6, 13 -- From zaluzec-at-microscopy.com Thu Nov 9 15:21:50 2006 6, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 6, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kA9LLnLE025324 6, 13 -- for {microscopy-at-microscopy.com} ; Thu, 9 Nov 2006 15:21:50 -0600 6, 13 -- Mime-Version: 1.0 6, 13 -- X-Sender: (Unverified) 6, 13 -- Message-Id: {p06110402c1794c59850a-at-[206.69.208.22]} 6, 13 -- Date: Thu, 9 Nov 2006 15:21:47 -0600 6, 13 -- To: microscopy-at-microscopy.com 6, 13 -- From: swtkeller-at-yahoo.com (by way of MicroscopyListserver) 6, 13 -- Subject: viaWWW: TEM-Looking for a general shallow junction etch recipe 6, 13 -- for silicon 6, 13 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
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Hi folks, I have a failure analysis job looking at a flip-chip Si die that has failed. It's been a (long) while since I did failure analysis looking at silicon chips and printed circuit boards.. What strategies are used to get a solder-bump chip off the board for further investigation? Various ploys using a soldering iron and tweezers come to mind but it would be nice to know how the experts do it, if you're willing to share the knowledge.
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==============================Original Headers============================== 9, 31 -- From richard.beanland-at-bookham.com Fri Nov 10 06:50:26 2006 9, 31 -- Received: from mail115.messagelabs.com (mail115.messagelabs.com [195.245.231.179]) 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kAACoPhP030876 9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 10 Nov 2006 06:50:26 -0600 9, 31 -- X-VirusChecked: Checked 9, 31 -- X-Env-Sender: richard.beanland-at-bookham.com 9, 31 -- X-Msg-Ref: server-9.tower-115.messagelabs.com!1163163024!16808388!1 9, 31 -- X-StarScan-Version: 5.5.10.7; banners=bookham.com,-,- 9, 31 -- X-Originating-IP: [213.249.209.179] 9, 31 -- Received: (qmail 10046 invoked from network); 10 Nov 2006 12:50:24 -0000 9, 31 -- Received: from unknown (HELO cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 9, 31 -- by server-9.tower-115.messagelabs.com with SMTP; 10 Nov 2006 12:50:24 -0000 9, 31 -- Received: from cas-smx-02.BOOKHAM.ENTERPRISE.PRI ([10.4.0.223]) by cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI with Microsoft SMTPSVC(6.0.3790.1830); 9, 31 -- Fri, 10 Nov 2006 12:54:27 +0000 9, 31 -- Content-class: urn:content-classes:message 9, 31 -- MIME-Version: 1.0 9, 31 -- Content-Type: text/plain; 9, 31 -- charset="us-ascii" 9, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 31 -- Subject: Failure analysis;flip-chip dies 9, 31 -- Date: Fri, 10 Nov 2006 12:54:30 -0000 9, 31 -- Message-ID: {9645D3E33E4C6548B12A7B25F611533E243B78-at-cas-smx-02.BOOKHAM.ENTERPRISE.PRI} 9, 31 -- X-MS-Has-Attach: 9, 31 -- X-MS-TNEF-Correlator: 9, 31 -- Thread-Topic: Failure analysis;flip-chip dies 9, 31 -- Thread-Index: AccEx10DsHw/ctWsTbCzJf2UytBnIg== 9, 31 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 9, 31 -- To: {microscopy-at-microscopy.com} 9, 31 -- X-OriginalArrivalTime: 10 Nov 2006 12:54:27.0385 (UTC) FILETIME=[5AED4290:01C704C7] 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAACoPhP030876 ==============================End of - Headers==============================
I would like to know the tricks for getting Word to write a number with a bar over it (as is used in crystallographic indices). I believe that this has been the subject of past posts and I have tried to find them in the archive but perhaps I am not using the right search terms. My apologies for bringing it up again.
I have had a solution that has served me well for a decade, but now I have a situation where it does not work. I recall that several solutions were proposed and I would like to try another. Help please.
If you are interested, what I have been doing until now is to create the symbol (eg bar-one) with the equation editor and then making an "autotext" entry to insert it.
Thanks, Alwyn -- ........... Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
==============================Original Headers============================== 5, 24 -- From jae5-at-lehigh.edu Fri Nov 10 07:47:51 2006 5, 24 -- Received: from rain.CC.Lehigh.EDU (rain.CC.Lehigh.EDU [128.180.39.20]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAADlo7W010196 5, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Nov 2006 07:47:51 -0600 5, 24 -- Received: from [127.0.0.1] (Dyn055141.mat.Lehigh.EDU [128.180.55.141]) 5, 24 -- (authenticated bits=0) 5, 24 -- by rain.CC.Lehigh.EDU (8.13.8/8.13.8) with ESMTP id kAADliKx014717 5, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 5, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Nov 2006 08:47:49 -0500 5, 24 -- Message-ID: {45548300.3090406-at-lehigh.edu} 5, 24 -- Date: Fri, 10 Nov 2006 08:47:44 -0500 5, 24 -- From: Alwyn Eades {jae5-at-lehigh.edu} 5, 24 -- Organization: Lehigh University 5, 24 -- User-Agent: Thunderbird 1.5.0.7 (Windows/20060909) 5, 24 -- MIME-Version: 1.0 5, 24 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com} 5, 24 -- Subject: Crystallographic indices 5, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- X-Virus-Scanned: ClamAV version 0.88.6, clamav-milter version 0.88.6 on rain.CC.Lehigh.EDU 5, 24 -- X-Virus-Status: Clean 5, 24 -- X-Spam-Status: No, score=-1.4 required=5.0 tests=ALL_TRUSTED 5, 24 -- autolearn=disabled version=3.1.7 5, 24 -- X-Spam-Checker-Version: SpamAssassin 3.1.7 (2006-10-05) on rain.CC.Lehigh.EDU ==============================End of - Headers==============================
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Email: ptomic-at-ciclonsemi.com Name: Peter Tomic
Organization: Ciclon Semiconductor Device Corp
Title-Subject: [Filtered] Failure analysis;flip-chip dies
Question: Richard,
A possible solution to this problem is a hot air stream system. If you know the solder composition, e.g. Pb-Sn or Pb-free, you can adjust the air temperature to accomodate. You're likely to fracture the device if you use a conventional soldering iron.
I have one other suggestion as an analysis tool prior to P.C. board removal. If the substrate is not too heavily doped, you may use NIR [Near Infra-Red] microscopy to image the device from the backside. Of course this is provided no NIR opaque materials are present. I'm assuming the device you are working with is Si, but NIR also works well for GaAs.
Regards,
Peter Tomic Ciclon Semiconductor Device Corp.
Hi folks, I have a failure analysis job looking at a flip-chip Si die that has failed. It's been a (long) while since I did failure analysis looking at silicon chips and printed circuit boards.. What strategies are used to get a solder-bump chip off the board for further investigation? Various ploys using a soldering iron and tweezers come to mind but it would be nice to know how the experts do it, if you're willing to share the knowledge.
Many thanks
Richard
________________________________________ Richard Beanland Materials Analysis Bookham Caswell Towcester Northants NN12 8EQ United Kingdom Tel. +44 1327 356362
Alwyn, One possible solution is to install in Windows a special crystallography fonts (crystallography.ttf) available at: http://x-seed.net/freestuff.html. I hope this helps, Leszek
Leszek Kepinski Division of Nanomaterials Chemistry and Catalysis Institute of Low Temperature and Structure Research, Polish Academy of Sciences, P.O. Box 1410, 50-950 Wroclaw, Poland e-mail: L.Kepinski-at-int.pan.wroc.pl
----- Original Message ----- X-from: {jae5-at-lehigh.edu} To: {L.Kepinski-at-int.pan.wroc.pl} Sent: Friday, November 10, 2006 2:50 PM
Alwyn, Word allows you to overwrite two characters in the same position by using one of the field code functions. To make "bar-1" you should:
(1) press ctrl-F9 (this will automatically insert two curly brackets { } in which you can type the field-code instruction; (2) inside the curly brackets type EQ \o(1,Ż) - you may need to play around to find the "overbar" symbol. On my system I can find this using Insert:Symbol (it is called the "Macron"). Alternatively you can try alt+0175 (using the number keypad). (3) press alt-F9 to display the text (you may need to do this twice). You can toggle between the field-code and the text using Alt-F9.
I seem to remember reading somewhere that for some language settings (where the decimal separator is defined as the comma?) you may need to use a semi-colon instead of a comma to separate the two characters (i.e. (1;Ż)).
----- Original Message ----- X-from: {jae5-at-lehigh.edu} To: {awgodfrey-at-mail.tsinghua.edu.cn} Sent: Friday, November 10, 2006 9:50 PM
Chuck,
I just would like to throw in a small clarification on AES as the posts seem to be a little unclears as far as heat usage.
In order to achieve UHV levels of vacuum, the systems have to be baked in order to get all the surfaces clean enough to get to that vacuum level. If you wanted a BSE detector in one of these, I would imagine it would have to be baked also, I don't see a way around that. However, the ability for the detector to withstand temperature, would be not when it's powered up but rather without any power to it. I don't know if this makes any difference with BSE detectors, but I thought it should be pointed out.
UHV is needed in AES so that the surfaces being analyzed are not contaminated by the gas molecules from the vacuum itself. In UHV, the time to form a monolayer of atoms deposited on the surface is a direct function of the level of vacuum acheived. Since AES is a surface analysis technique, meaning the signal is generated from the top 2 or 3 atomic layers (IIRC), it is imperative to be able to keep the surface clean during analysis.
How to achieve a "clean" surface for AES analysis can be complicated, however, I have not seen heating to be a normally used method except in special cases.
The BSE detctor would not have to be able to withstand heat while operating, only while idle and with no power to it.
dj
On Fri, 10 Nov 2006, cgarber-at-2spi.com wrote:
} It has been my understanding that temperature can at times be important in } an Auger system because the energy of Auger electrons is low and the Auger } electrons come from very close to the surface, perhaps the top nm or so. If } the surface is contaminated with a gas, or some other surface-sorbed } species, the Auger electrons, which are related to the material composition } of the surface, would only generate a signal from the contamination layer, } not the material underneath that it was intended to study. The conventinal } wisdom is that UHV and bake out assure low contamination at the surface to } be analyzed. } } Auger electrons also have a typical energy of 1 - 2 kV and as such are best } excited by lower energy electron beams, usually less than 5 kV, and } a BSE detector that responds well to low kV electrons is required for BSE } imaging at those voltages. } } None of the Robinson designed detectors, including the latest versions is } good for heating and bakeout. I am reasonably certain this would be the } case for any other BSE detector but of course, I could be wrong about that. } For the Robinson BSE detectors, the maximum temperature they will withstand } is slightly under 50 C. } } I presume Dr. Robinson might be following this conversation and perhaps he } can explain this better. } } Disclaimer: My firm, SPI Supplies is the North American distributor for the } Robinson BSE detector product line so we are not a disinterested third } party. } } Chuck } } =================================================== } Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 } President } SPI SUPPLIES FAX: 1-(610)-436-5755 } PO BOX 656 e-mail: cgarber-at-2spi.com } West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com } } } Look for us! } ############################ } WWW: http://www.2spi.com } ############################ } ================================================== } } } } ==============================Original Headers============================== } 11, 27 -- From cgarber-at-2spi.com Fri Nov 10 00:43:07 2006 } 11, 27 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) } 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAA6h6PR032238 } 11, 27 -- for {microscopy-at-msa.microscopy.com} ; Fri, 10 Nov 2006 00:43:07 -0600 } 11, 27 -- Received: from yourb27fb1c401 (c-71-230-36-118.hsd1.pa.comcast.net [71.230.36.118]) } 11, 27 -- (authenticated bits=0) } 11, 27 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id kAA6biNP016895; } 11, 27 -- Fri, 10 Nov 2006 01:37:51 -0500 } 11, 27 -- X-IDV-FirstRcvd: c-71-230-36-118.hsd1.pa.comcast.net [71.230.36.118] } 11, 27 -- X-IDV-HELO: yourb27fb1c401 } 11, 27 -- X-IDV-Authenticated-User: cgarber } 11, 27 -- Message-ID: {00ff01c70492$bb974da0$6401a8c0-at-yourb27fb1c401} } 11, 27 -- From: "Garber, Charles A" {cgarber-at-2spi.com} } 11, 27 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} } 11, 27 -- References: {200611092155.kA9LtFHu023763-at-ns.microscopy.com} } 11, 27 -- Subject: BSE and EBSD detectors } 11, 27 -- Date: Fri, 10 Nov 2006 01:37:39 -0500 } 11, 27 -- MIME-Version: 1.0 } 11, 27 -- Content-Type: text/plain; } 11, 27 -- format=flowed; } 11, 27 -- charset="iso-8859-1"; } 11, 27 -- reply-type=original } 11, 27 -- Content-Transfer-Encoding: 7bit } 11, 27 -- X-Priority: 3 } 11, 27 -- X-MSMail-Priority: Normal } 11, 27 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2869 } 11, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 } ==============================End of - Headers============================== }
==============================Original Headers============================== 11, 19 -- From dljones-at-bestweb.net Fri Nov 10 08:33:49 2006 11, 19 -- Received: from vms040pub.verizon.net (vms040pub.verizon.net [206.46.252.40]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAAEXni9020897 11, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Nov 2006 08:33:49 -0600 11, 19 -- Received: from localhost ([71.249.18.226]) 11, 19 -- by vms040.mailsrvcs.net (Sun Java System Messaging Server 6.2-6.01 (built Apr 11, 19 -- 3 2006)) with ESMTPA id {0J8I00K2KR3G1LO4-at-vms040.mailsrvcs.net} for 11, 19 -- Microscopy-at-microscopy.com; Fri, 10 Nov 2006 08:33:21 -0600 (CST) 11, 19 -- Date: Fri, 10 Nov 2006 09:46:24 -0500 (Eastern Standard Time) 11, 19 -- From: "David L. Jones" {dljones-at-bestweb.net} 11, 19 -- Subject: Re: [Microscopy] BSE and EBSD detectors 11, 19 -- In-reply-to: {200611100647.kAA6l98J006238-at-ns.microscopy.com} 11, 19 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 11, 19 -- To: cgarber-at-2spi.com 11, 19 -- Cc: Microscopy-at-microscopy.com 11, 19 -- Message-id: {Pine.WNT.4.64.0611100918540.1984-at-H-F1} 11, 19 -- MIME-version: 1.0 11, 19 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 11, 19 -- References: {200611100647.kAA6l98J006238-at-ns.microscopy.com} ==============================End of - Headers==============================
Could someone please explain the difference between phase contrast and dark field microcopy? What optic effects one get with one or the other? I see Frank Karl states in his very nice explanation of dark field sent to the list the following: "One easy short cut is to use a phase contrast scope and select a condenser phase ring larger than the ring in your objective". Can Phase contrast be transformed into darkfield then?
Thank you for any explanation
Antonio M Garcia
==============================Original Headers============================== 4, 22 -- From antoniomiguel.garcia-at-gmail.com Fri Nov 10 08:35:01 2006 4, 22 -- Received: from nf-out-0910.google.com (nf-out-0910.google.com [64.233.182.189]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAAEZ0xv023355 4, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Nov 2006 08:35:00 -0600 4, 22 -- Received: by nf-out-0910.google.com with SMTP id i2so1259922nfe 4, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Nov 2006 06:34:59 -0800 (PST) 4, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 4, 22 -- s=beta; d=gmail.com; 4, 22 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 22 -- b=qBFefrWuTv3IWzvNp1ZF8HbTVqo3g4aut/8tcZmpXvdrPU1Ht/+dTIizb+WnYe3ADfS4ao5Zp5cINzc3ZBTsAel0e4exv90YBcoN7NH3dBWM7ZDVMx4ximM+OIwuEUD6MUlgebaXMp/14FNqw/GgQ/mwqRZsoVorvJZJTWc90/8= 4, 22 -- Received: by 10.48.48.13 with SMTP id v13mr5455318nfv.1163169299662; 4, 22 -- Fri, 10 Nov 2006 06:34:59 -0800 (PST) 4, 22 -- Received: by 10.48.245.18 with HTTP; Fri, 10 Nov 2006 06:34:59 -0800 (PST) 4, 22 -- Message-ID: {703e88850611100634hddc2e5bofe27d044f29114e6-at-mail.gmail.com} 4, 22 -- Date: Fri, 10 Nov 2006 15:34:59 +0100 4, 22 -- From: "=?ISO-8859-1?Q?Antonio_Miguel_Garc=EDa?=" {antoniomiguel.garcia-at-gmail.com} 4, 22 -- To: Microscopy-at-microscopy.com 4, 22 -- Subject: AskAMicroscopist:Phase contrast vs Dark Field 4, 22 -- MIME-Version: 1.0 4, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- Content-Disposition: inline ==============================End of - Headers==============================
Antonio, Darkfield allows you to detect objects by virtue of their ability to scatter light. Scattering is weak so you have to "get rid" of the non-scattered, incident light. The way you do this is to have the light incident at an angle that is too wide for the lens to gather. Imagine a hollow cone of light coming up through the condenser that is at an angle too wide for the NA of the objective. This hollow cone of light converges on the specimen and carries on past the objective. If the specimen is empty then looking down the tubes you see darkness. That is where darkfield gets its name. But if you have some scatterers, you will see light from them against the dark background, and the more they scatter the brighter they will be. Darkfield is great for picking up dust and debris, among other things. That's the concept. Now, how do you get a hollow cone of light? Well you can see that the diffculty depends on the NA. The higher the NA of your objective the trickier the condenser design needs to be to achive an angle greater than the objective NA. So if you have a modest to low power objective, then a quick and easy way to get such a hollow cone is to use a phase ring designed for a high NA lens. Phase rings exactly provide hollow cones of light and by using a phase ring designed for a high NA objective (ie wide light cone) with a low power objective, the hollow cone of incident light passes that objective by and you get darkfield. Obviously, this approach won't work if you want to do darkfield with a high NA lens, and for that you need a specially designed darkfield condensor.
Now, phase contrast allows you do detect objects by virtue of their optical thickness, which changes the phase of the light passing through them compared to light that does not pass through them. The tricky bit is to convert that into something your eye can see. This is done also with a hollow cone of light but in this case the cone is gathered by the objective and in the rear focal plane of the lens is placed a ring that lines up with the cone. The ring in the obj rear focal plane changes the phase of this incident light, relative any light that goes elsewhere through the rear focal plane of the objective. That is ultimately how the phase difference between light that goes through the sample (and hence elsewhere in the rear focal plane) and the background (light through the phase ring) is detected.
The optics behind phase contrast are much trickier to grasp intuitively, indeed Zernicke won a Nobel prize for inventing it, whereas darkfield has been played with for ages.
hope this helps, Tobias
} } } Hello } } Could someone please explain the difference between phase contrast and dark } field microcopy? What optic effects one get with one or the other? I see Frank } Karl states in his very nice explanation of dark field sent to the list the } following: "One easy short cut is to use a phase contrast scope and select } a condenser phase ring larger than the ring in your objective". Can } Phase contrast } be transformed into darkfield then? } } Thank you for any explanation } } Antonio M Garcia }
At the risk of creating another bump and run... Phase contrast and dark field are different. Phase contrast converts phase differences between the object of interest and the surrounding material to amplitude difference which is what the human eye is sensitive to. One could think of darkfield as a way of increase detectability.
Using a large phase ring in the condenser is a trick. Just a short cut to get illuminated objects on a dark background.
I refer you to Tobias's excellent explanation.
antoniomiguel.garci a-at-gmail.com To: frank.karl-at-degussa.com cc: 11/10/2006 08:35 AM Subject: [Microscopy] AskAMicroscopist:Phase contrast vs Dark Field Please respond to antoniomiguel.garci a
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hello
Could someone please explain the difference between phase contrast and dark field microcopy? What optic effects one get with one or the other? I see Frank Karl states in his very nice explanation of dark field sent to the list the following: "One easy short cut is to use a phase contrast scope and select a condenser phase ring larger than the ring in your objective". Can Phase contrast be transformed into darkfield then?
Thank you for any explanation
Antonio M Garcia
==============================Original Headers============================== 4, 22 -- From antoniomiguel.garcia-at-gmail.com Fri Nov 10 08:35:01 2006 4, 22 -- Received: from nf-out-0910.google.com (nf-out-0910.google.com [64.233.182.189]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAAEZ0xv023355 4, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Nov 2006 08:35:00 -0600 4, 22 -- Received: by nf-out-0910.google.com with SMTP id i2so1259922nfe 4, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Nov 2006 06:34:59 -0800 (PST) 4, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 4, 22 -- s=beta; d=gmail.com; 4, 22 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition;
We have two LKB 7801A knifebreakers which I have been able to bring back up to full functionality, with the help of a lot of useful replies to a fairly recent posting of mine on this listserver. But I have one last question.
When you take the top off the machine, you can see the four heavy screws which hold the breaking head onto its heavy metal base (I'm not talking guitars here). But there are two other, lighter allen-head screws that appear to be there to adjust the height of the breaking head off the metal base when the four heavier screws are tightened down. Is that actually what they are for? Is this to adjust the amount of pressure necessary to break glass?
Thanks for any hints. This is fix-everything-broken week in our lab....
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 7, 23 -- From TindallR-at-missouri.edu Fri Nov 10 09:03:58 2006 7, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAAF3vZZ029981 7, 23 -- for {microscopy-at-microscopy.com} ; Fri, 10 Nov 2006 09:03:57 -0600 7, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 7, 23 -- Fri, 10 Nov 2006 09:03:57 -0600 7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="us-ascii" 7, 23 -- Subject: Knifebreaker question 7, 23 -- Date: Fri, 10 Nov 2006 09:03:56 -0600 7, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68B7C-at-UM-XMAIL08.um.umsystem.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- Thread-Topic: Knifebreaker question 7, 23 -- Thread-Index: AccE2XKSqTGHReuzTz+BnsM/bXsOFQ== 7, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 7, 23 -- To: {microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 10 Nov 2006 15:03:57.0012 (UTC) FILETIME=[71FC4140:01C704D9] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAAF3vZZ029981 ==============================End of - Headers==============================
Can anybody recommend a good primary antibody against rhodopsin in mice and/or dog retina? Preferably one that has been tested for EM immunogold labeling? My perusal of the online catalogs hasn't been very helpful so far.
Thanks much.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 6, 23 -- From TindallR-at-missouri.edu Fri Nov 10 10:13:13 2006 6, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAAGDDup011168 6, 23 -- for {microscopy-at-microscopy.com} ; Fri, 10 Nov 2006 10:13:13 -0600 6, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 6, 23 -- Fri, 10 Nov 2006 10:13:12 -0600 6, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 23 -- Content-class: urn:content-classes:message 6, 23 -- MIME-Version: 1.0 6, 23 -- Content-Type: text/plain; 6, 23 -- charset="us-ascii" 6, 23 -- Subject: TEM: Anti-rhodopsin ab 6, 23 -- Date: Fri, 10 Nov 2006 10:13:13 -0600 6, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68B80-at-UM-XMAIL08.um.umsystem.edu} 6, 23 -- X-MS-Has-Attach: 6, 23 -- X-MS-TNEF-Correlator: 6, 23 -- Thread-Topic: TEM: Anti-rhodopsin ab 6, 23 -- Thread-Index: AccE4x73PtzboLgUTfqUI9p9BKoOnw== 6, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 6, 23 -- To: {microscopy-at-microscopy.com} 6, 23 -- X-OriginalArrivalTime: 10 Nov 2006 16:13:12.0731 (UTC) FILETIME=[1EFCA6B0:01C704E3] 6, 23 -- Content-Transfer-Encoding: 8bit 6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAAGDDup011168 ==============================End of - Headers==============================
It would depend a great deal on the type of failure mode, or mechanism, you suspect. I have used a hot air heat gun to melt the solder balls and taped the part off the board. I have carefully cut away most of the circuit board, and then ground away the circuit board directly under the module, which would still be attached by the solder balls. I have also used single edge razor blades, or knife blades. Do you plan on powering up the module (or chip), and testing it, after you get it off the board, or is this strictly a physical failure analysis?
The sample prep must not alter/affect the failure, or the clues that are going help solve the puzzle. Heat can be a problem, as well as the water drip while grinding away the circuit board (may need to be done dry, which is difficult with ceramic substrates).
I hope this helps. If there are more specific questions, I will try to help. My 20+ years in FA has been varied, and quite interesting.
Darrell
richard.beanland-at-bookham.com wrote on 11/10/2006 07:51:52 AM:
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} } Hi folks, } I have a failure analysis job looking at a flip-chip Si die that } has failed. It's been a (long) while since I did failure analysis } looking at silicon chips and printed circuit boards.. What strategies } are used to get a solder-bump chip off the board for further } investigation? Various ploys using a soldering iron and tweezers come } to mind but it would be nice to know how the experts do it, if you're } willing to share the knowledge. } } Many thanks } } Richard } } } } ________________________________________ } Richard Beanland } Materials Analysis } Bookham } Caswell } Towcester } Northants } NN12 8EQ } United Kingdom } Tel. +44 1327 356362 } Fax. +44 1327 356775 } http://www.bookham.com } ________________________________________ } } } } ======================================================================= } This e-mail is intended for the person it is addressed to only. The } information contained in it may be confidential and/or protected by } law. If you are not the intended recipient of this message, you must } not make any use of this information, or copy or show it to any } person. Please contact us immediately to tell us that you have } received this e-mail, and return the original to us. Any use, } forwarding, printing or copying of this message is strictly prohibited. } No part of this message can be considered a request for goods or } services. } ======================================================================= } } } ==============================Original Headers============================== } 9, 31 -- From richard.beanland-at-bookham.com Fri Nov 10 06:50:26 2006 } 9, 31 -- Received: from mail115.messagelabs.com (mail115. } messagelabs.com [195.245.231.179]) } 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id kAACoPhP030876 } 9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 10 Nov 2006 06:50:26 -0600 } 9, 31 -- X-VirusChecked: Checked } 9, 31 -- X-Env-Sender: richard.beanland-at-bookham.com } 9, 31 -- X-Msg-Ref: server-9.tower-115.messagelabs.com!1163163024!16808388!1 } 9, 31 -- X-StarScan-Version: 5.5.10.7; banners=bookham.com,-,- } 9, 31 -- X-Originating-IP: [213.249.209.179] } 9, 31 -- Received: (qmail 10046 invoked from network); 10 Nov 2006 } 12:50:24 -0000 } 9, 31 -- Received: from unknown (HELO cas-smx-brdg-02.BOOKHAM. } ENTERPRISE.PRI) (213.249.209.179) } 9, 31 -- by server-9.tower-115.messagelabs.com with SMTP; 10 Nov } 2006 12:50:24 -0000 } 9, 31 -- Received: from cas-smx-02.BOOKHAM.ENTERPRISE.PRI ([10.4.0. } 223]) by cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI with Microsoft } SMTPSVC(6.0.3790.1830); } 9, 31 -- Fri, 10 Nov 2006 12:54:27 +0000 } 9, 31 -- Content-class: urn:content-classes:message } 9, 31 -- MIME-Version: 1.0 } 9, 31 -- Content-Type: text/plain; } 9, 31 -- charset="us-ascii" } 9, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 9, 31 -- Subject: Failure analysis;flip-chip dies } 9, 31 -- Date: Fri, 10 Nov 2006 12:54:30 -0000 } 9, 31 -- Message-ID: {9645D3E33E4C6548B12A7B25F611533E243B78-at-cas- } smx-02.BOOKHAM.ENTERPRISE.PRI} } 9, 31 -- X-MS-Has-Attach: } 9, 31 -- X-MS-TNEF-Correlator: } 9, 31 -- Thread-Topic: Failure analysis;flip-chip dies } 9, 31 -- Thread-Index: AccEx10DsHw/ctWsTbCzJf2UytBnIg== } 9, 31 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} } 9, 31 -- To: {microscopy-at-microscopy.com} } 9, 31 -- X-OriginalArrivalTime: 10 Nov 2006 12:54:27.0385 (UTC) } FILETIME=[5AED4290:01C704C7] } 9, 31 -- Content-Transfer-Encoding: 8bit } 9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns. } microscopy.com id kAACoPhP030876 } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 28 -- From milesd-at-us.ibm.com Fri Nov 10 10:59:05 2006 10, 28 -- Received: from e6.ny.us.ibm.com (e6.ny.us.ibm.com [32.97.182.146]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAAGx41h022762 10, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 10 Nov 2006 10:59:04 -0600 10, 28 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236]) 10, 28 -- by e6.ny.us.ibm.com (8.13.8/8.12.11) with ESMTP id kAAGxLQ0024998 10, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 10 Nov 2006 11:59:21 -0500 10, 28 -- Received: from d01av03.pok.ibm.com (d01av03.pok.ibm.com [9.56.224.217]) 10, 28 -- by d01relay04.pok.ibm.com (8.13.6/8.13.6/NCO v8.1.1) with ESMTP id kAAGwZwJ073774 10, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 10 Nov 2006 11:58:37 -0500 10, 28 -- Received: from d01av03.pok.ibm.com (loopback [127.0.0.1]) 10, 28 -- by d01av03.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id kAAGwZ6a011359 10, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 10 Nov 2006 11:58:35 -0500 10, 28 -- Received: from d01ml076.pok.ibm.com (d01ml076.pok.ibm.com [9.56.229.50]) 10, 28 -- by d01av03.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id kAAGwY1Y011343; 10, 28 -- Fri, 10 Nov 2006 11:58:34 -0500 10, 28 -- In-Reply-To: {200611101251.kAACpqON032202-at-ns.microscopy.com} 10, 28 -- Subject: Re: [Microscopy] Failure analysis;flip-chip dies 10, 28 -- To: richard.beanland-at-bookham.com 10, 28 -- Cc: Microscopy-at-MSA.Microscopy.Com 10, 28 -- X-Mailer: Lotus Notes Release 7.0 HF277 June 21, 2006 10, 28 -- Message-ID: {OF87F6222F.356C316C-ON85257222.005A9C65-85257222.005D407F-at-us.ibm.com} 10, 28 -- From: Darrell Miles {milesd-at-us.ibm.com} 10, 28 -- Date: Fri, 10 Nov 2006 11:58:33 -0500 10, 28 -- X-MIMETrack: Serialize by Router on D01ML076/01/M/IBM(Release 7.0.1FP1 HF1|June 27, 2006) at 10, 28 -- 11/10/2006 11:58:34 10, 28 -- MIME-Version: 1.0 10, 28 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Sent: Friday, November 10, 2006 7:52 AM To: White, Woody N.
Hi folks, I have a failure analysis job looking at a flip-chip Si die that has failed. It's been a (long) while since I did failure analysis looking at silicon chips and printed circuit boards.. What strategies are used to get a solder-bump chip off the board for further investigation? Various ploys using a soldering iron and tweezers come to mind but it would be nice to know how the experts do it, if you're willing to share the knowledge.
======================================================================= This e-mail is intended for the person it is addressed to only. The information contained in it may be confidential and/or protected by law. If you are not the intended recipient of this message, you must not make any use of this information, or copy or show it to any person. Please contact us immediately to tell us that you have received this e-mail, and return the original to us. Any use, forwarding, printing or copying of this message is strictly prohibited. No part of this message can be considered a request for goods or services. =======================================================================
==============================Original Headers============================== 9, 31 -- From richard.beanland-at-bookham.com Fri Nov 10 06:50:26 2006 9, 31 -- Received: from mail115.messagelabs.com (mail115.messagelabs.com [195.245.231.179]) 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kAACoPhP030876 9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 10 Nov 2006 06:50:26 -0600 9, 31 -- X-VirusChecked: Checked 9, 31 -- X-Env-Sender: richard.beanland-at-bookham.com 9, 31 -- X-Msg-Ref: server-9.tower-115.messagelabs.com!1163163024!16808388!1 9, 31 -- X-StarScan-Version: 5.5.10.7; banners=bookham.com,-,- 9, 31 -- X-Originating-IP: [213.249.209.179] 9, 31 -- Received: (qmail 10046 invoked from network); 10 Nov 2006 12:50:24 -0000 9, 31 -- Received: from unknown (HELO cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 9, 31 -- by server-9.tower-115.messagelabs.com with SMTP; 10 Nov 2006 12:50:24 -0000 9, 31 -- Received: from cas-smx-02.BOOKHAM.ENTERPRISE.PRI ([10.4.0.223]) by cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI with Microsoft SMTPSVC(6.0.3790.1830); 9, 31 -- Fri, 10 Nov 2006 12:54:27 +0000 9, 31 -- Content-class: urn:content-classes:message 9, 31 -- MIME-Version: 1.0 9, 31 -- Content-Type: text/plain; 9, 31 -- charset="us-ascii" 9, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 31 -- Subject: Failure analysis;flip-chip dies 9, 31 -- Date: Fri, 10 Nov 2006 12:54:30 -0000 9, 31 -- Message-ID: {9645D3E33E4C6548B12A7B25F611533E243B78-at-cas-smx-02.BOOKHAM.ENTERPRISE.PR I} 9, 31 -- X-MS-Has-Attach: 9, 31 -- X-MS-TNEF-Correlator: 9, 31 -- Thread-Topic: Failure analysis;flip-chip dies 9, 31 -- Thread-Index: AccEx10DsHw/ctWsTbCzJf2UytBnIg== 9, 31 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 9, 31 -- To: {microscopy-at-microscopy.com} 9, 31 -- X-OriginalArrivalTime: 10 Nov 2006 12:54:27.0385 (UTC) FILETIME=[5AED4290:01C704C7] 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAACoPhP030876 ==============================End of - Headers==============================
==============================Original Headers============================== 20, 27 -- From nwwhite-at-bwxt.com Fri Nov 10 12:10:19 2006 20, 27 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 20, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAAIAIJl002715 20, 27 -- for {microscopy-at-msa.microscopy.com} ; Fri, 10 Nov 2006 12:10:19 -0600 20, 27 -- Received: from ([131.184.13.224]) 20, 27 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.2751614; 20, 27 -- Fri, 10 Nov 2006 13:10:00 -0500 20, 27 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.30]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); 20, 27 -- Fri, 10 Nov 2006 12:51:20 -0500 20, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 20, 27 -- Content-class: urn:content-classes:message 20, 27 -- MIME-Version: 1.0 20, 27 -- Content-Type: text/plain; 20, 27 -- charset="us-ascii" 20, 27 -- Subject: RE: [Microscopy] Failure analysis;flip-chip dies 20, 27 -- Date: Fri, 10 Nov 2006 12:48:16 -0500 20, 27 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB703D0D5-at-BWXSPO01.BWXS.BWXTECH.NET} 20, 27 -- X-MS-Has-Attach: 20, 27 -- X-MS-TNEF-Correlator: 20, 27 -- Thread-Topic: [Microscopy] Failure analysis;flip-chip dies 20, 27 -- Thread-Index: AccEyK3fWatR3WJFSrqmIdB25W9RbQAJ0LJg 20, 27 -- From: "White, Woody N." {NWWhite-at-bwxt.com} 20, 27 -- To: {richard.beanland-at-bookham.com} , 20, 27 -- "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 20, 27 -- X-OriginalArrivalTime: 10 Nov 2006 17:51:20.0762 (UTC) FILETIME=[D486E1A0:01C704F0] 20, 27 -- Content-Transfer-Encoding: 8bit 20, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAAIAIJl002715 ==============================End of - Headers==============================
Richard; A lot depends on the exact method of manufacturing of the flip chip part. Every manufacturer does something a little different and then patents their method. Epoxy under fills are popular for taking thermally induced stresses away from the solder bumps. In that case, no amount of hot anything will get the part off. All analysis must be done from the back side. If this sounds difficult, it is. There are a lot of backside thinning techniques that have been developed to address this difficulty. They consume extensive resources but they do work. However, many are proprietary, and cannot be revealed on this forum.
John Mardinly Intel
This is the opinion of the author and does not necessarily represent the opinion of Intel Corp.
Sent: Friday, November 10, 2006 4:51 AM To: Mardinly, John
Hi folks, I have a failure analysis job looking at a flip-chip Si die that has failed. It's been a (long) while since I did failure analysis looking at silicon chips and printed circuit boards.. What strategies are used to get a solder-bump chip off the board for further investigation? Various ploys using a soldering iron and tweezers come to mind but it would be nice to know how the experts do it, if you're willing to share the knowledge.
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==============================Original Headers============================== 9, 31 -- From richard.beanland-at-bookham.com Fri Nov 10 06:50:26 2006 9, 31 -- Received: from mail115.messagelabs.com (mail115.messagelabs.com [195.245.231.179]) 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kAACoPhP030876 9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 10 Nov 2006 06:50:26 -0600 9, 31 -- X-VirusChecked: Checked 9, 31 -- X-Env-Sender: richard.beanland-at-bookham.com 9, 31 -- X-Msg-Ref: server-9.tower-115.messagelabs.com!1163163024!16808388!1 9, 31 -- X-StarScan-Version: 5.5.10.7; banners=bookham.com,-,- 9, 31 -- X-Originating-IP: [213.249.209.179] 9, 31 -- Received: (qmail 10046 invoked from network); 10 Nov 2006 12:50:24 -0000 9, 31 -- Received: from unknown (HELO cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 9, 31 -- by server-9.tower-115.messagelabs.com with SMTP; 10 Nov 2006 12:50:24 -0000 9, 31 -- Received: from cas-smx-02.BOOKHAM.ENTERPRISE.PRI ([10.4.0.223]) by cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI with Microsoft SMTPSVC(6.0.3790.1830); 9, 31 -- Fri, 10 Nov 2006 12:54:27 +0000 9, 31 -- Content-class: urn:content-classes:message 9, 31 -- MIME-Version: 1.0 9, 31 -- Content-Type: text/plain; 9, 31 -- charset="us-ascii" 9, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 31 -- Subject: Failure analysis;flip-chip dies 9, 31 -- Date: Fri, 10 Nov 2006 12:54:30 -0000 9, 31 -- Message-ID: {9645D3E33E4C6548B12A7B25F611533E243B78-at-cas-smx-02.BOOKHAM.ENTERPRISE.PR I} 9, 31 -- X-MS-Has-Attach: 9, 31 -- X-MS-TNEF-Correlator: 9, 31 -- Thread-Topic: Failure analysis;flip-chip dies 9, 31 -- Thread-Index: AccEx10DsHw/ctWsTbCzJf2UytBnIg== 9, 31 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 9, 31 -- To: {microscopy-at-microscopy.com} 9, 31 -- X-OriginalArrivalTime: 10 Nov 2006 12:54:27.0385 (UTC) FILETIME=[5AED4290:01C704C7] 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAACoPhP030876 ==============================End of - Headers==============================
==============================Original Headers============================== 19, 31 -- From john.mardinly-at-intel.com Fri Nov 10 12:24:09 2006 19, 31 -- Received: from mga03.intel.com (mga03.intel.com [143.182.124.21]) 19, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAAIO8So013467 19, 31 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 10 Nov 2006 12:24:09 -0600 19, 31 -- Received: from azsmga001.ch.intel.com ([10.2.17.19]) 19, 31 -- by mga03.intel.com with ESMTP; 10 Nov 2006 10:24:08 -0800 19, 31 -- Received: from scsmsx331.sc.intel.com (HELO scsmsx331.amr.corp.intel.com) ([10.3.90.4]) 19, 31 -- by azsmga001.ch.intel.com with ESMTP; 10 Nov 2006 10:24:07 -0800 19, 31 -- X-ExtLoop1: 1 19, 31 -- X-IronPort-AV: i="4.09,410,1157353200"; 19, 31 -- d="scan'208"; a="144318428:sNHT45184867" 19, 31 -- Received: from scsmsx413.amr.corp.intel.com ([10.3.90.32]) by scsmsx331.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.211); 19, 31 -- Fri, 10 Nov 2006 10:24:07 -0800 19, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 31 -- Content-class: urn:content-classes:message 19, 31 -- MIME-Version: 1.0 19, 31 -- Content-Type: text/plain; 19, 31 -- charset="us-ascii" 19, 31 -- Subject: RE: [Microscopy] Failure analysis;flip-chip dies 19, 31 -- Date: Fri, 10 Nov 2006 10:24:06 -0800 19, 31 -- Message-ID: {1DF4C4D62339DB4C9C98DF04213995B601BEBD2D-at-scsmsx413.amr.corp.intel.com} 19, 31 -- X-MS-Has-Attach: 19, 31 -- X-MS-TNEF-Correlator: 19, 31 -- Thread-Topic: [Microscopy] Failure analysis;flip-chip dies 19, 31 -- Thread-Index: AccExtPcbIhcvQzoQdyMy+YmQEJAhwALLuuA 19, 31 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 19, 31 -- To: {richard.beanland-at-bookham.com} 19, 31 -- Cc: {Microscopy-at-msa.microscopy.com} 19, 31 -- X-OriginalArrivalTime: 10 Nov 2006 18:24:07.0373 (UTC) FILETIME=[68B7E7D0:01C704F5] 19, 31 -- Content-Transfer-Encoding: 8bit 19, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAAIO8So013467 ==============================End of - Headers==============================
This can be (and usually is) a tricky proposition. It would help knowing a few details about your situation before plunging in to strategies.
First off, why do you need to do failure analysis on this IC? This is not meant to sound flippant. If you are really having to do FA on this IC, depending on its complexity, it can be a huge job and even bigger if you are not set up to do this sort of work on a more routine basis. Even so, different types of ICs tend to need different methods of FA. Based on un-installed identical ICs, what type of metal interconnects are used (Al or Cu), how many layers, ILD type and what method of planarization? Is this flip chip on a supporting substrate or flipped directly onto the main PCB? Is there any conformal coating on the board/IC?
What part number is the IC? This will tell what kind of base package it is--plastic or ceramic and the node size of the IC and if it is Pb-free or not. What kind of PCB is the part on? Is it multi-layer FR4 or ? What is the requirement for retaining the main PCB--i.e., can it be destroyed? Once removed from the main PCB, are you going to re-bump the package and do electrical testing? This would be a first look step to test the IC. The problem with this is that depending on the construction of the whole part, the die and/or package could be destroyed such that the whole thing would no longer work electrically.
gary g.
At 04:53 AM 11/10/2006, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Fri Nov 10 12:50:38 2006 11, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kAAIobdZ024534 11, 20 -- for {microscopy-at-microscopy.com} ; Fri, 10 Nov 2006 12:50:38 -0600 11, 20 -- Received: (qmail 6249 invoked from network); 10 Nov 2006 10:50:36 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 6195, pid: 6244, t: 0.1167s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 20 -- by qsmtp3 with SMTP; 10 Nov 2006 10:50:36 -0800 11, 20 -- Message-Id: {7.0.1.0.2.20061110103602.024d4cb8-at-gaugler.com} 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 11, 20 -- Date: Fri, 10 Nov 2006 10:50:31 -0800 11, 20 -- To: richard.beanland-at-bookham.com 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] Failure analysis;flip-chip dies 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200611101253.kAACrPpa001481-at-ns.microscopy.com} 11, 20 -- References: {200611101253.kAACrPpa001481-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
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Please see the following paper by Dr. Charles W. Pearce, "Defect Delineation and Junction Staining Techniques for Silicon Using Wet Chemistry", AT&T, Allentown, PA presented at the Electrochemical Society, October 1991. The author is just a few doors down from me right now so I can get you questions answered from the source, if need be.
The paper outlines and provides recipes for Sirtl, Secco, Wright-Jenkins, Yang's Etch, Schimmel, Seiter, Dash, Sato and Memc.
Hi Sandra, As far as I'm aware, the only junction delineation stain that has been used successfully for TEM work is 0.5% HF in Nitric. This has been a well- established technique for looking at junctions in Si for years. A fairly recent example for shallow junctions is given in Yoo et al., Appl. Phys. Letts. 80, 2687 (2002). You do have to take some precautions to get a good result. For example, if your sample is mounted on a Cu grid, you have to protect this from the nitric acid by coating it with lacquer or something similar. I seem to remember Ron Anderson telling me he used to cool the etchant well below room temperature to slow the etch rate down and make it easier to control. HF is a particularly nasty acid, so if you have no experience of it make sure you are properly trained in its use before you start.
I'll add another perspective on their descriptions:
Dark Field:
The cone of light that goes through the sample coming out of the condenser is more like a thin annulus. Think of it as a party hat (thin outer rim coming to a point). It is at a very high angle (outside the field of view). The angle that exits the sample layer is too high to be captured by the objective. Think of a party hat on top of another party hat point-to-point. The brim of the top party hat (that should get captured by the objective) is too big. Thus, when no sample is in the light path, the field of view is dark (dakfield). Small specs or the edges of samples (high change in phase) scatter the light so that it changes angle of the rays at that point and does get captured by the objective. One then sees white edges or spots (where this occurs in the sample) over a darkfield (where there is no scattering).
Phase Contrast:
In the most common use, the cone of light that goes through the sample coming out of the condenser is a broad annulus. Think of it as a party hat made out of very thick cardboard (outer rim coming to a point). It is at a moderate angle (inside the field of view). As the light passes through the specimen there are a) rays that are normally seen and do not touch any of the specimen and b) there are some that go through the specimen. The ones that go through the specimen are retarded (many biological specimens retard by 1/4 of a wavelength of light or within a wavelength range). As they come back through the objective, some encounter a dark (quasi opaque) annulus in the back focal plane that matches the optical size of the annulus of light from the condenser. This is called a phase ring. The phase ring set-up is of the correct thickness to either retard the rays by 1/4 of a wavelngth (positive phase contrast) or to advance them (negative phase contrast). The rays going through the specimen are then doubly retarded - hopefully many by 1/4 plus 1/4 = 1/2 wavelength, while other rays are not. When they recombine, those rays that are 1/2 wavelength in difference (out of phase) cancel each other producing darkness. Those that are in phase combine to be bright. This dark (black) vs bright differences provide contrast - hence phase contrast. The actual retardation (phase shift) is dependent upon thickness and refractive indices.
(For positive phase contrast) because many rays do not get their phase shifted, the background is bright with objects or edges dark. For negative phase contrast, the background is dark and the objects are light.
Let's take a phase annulus for phase contrast setup. Let's presume it was designed for an objective of higher numerical aperature (say NA = 0.85 for a 100X obj). If we use an 40X objective with an NA of 0.65, the only the light scattered from the specimen will reach the objective. This will create a darkfield effect. It will not be the optimum though - only the proper sizing will do that.
Another comment, because phase differences are related to thickness, one can see artificial repetitive lines (series or halos) from where thicknesses create phase shifts of 1/4 wavelength plus 1 wavelength, 1/4 wavelength plus 2 wavelengths, 1/4 wavelength plus 3 wavelengths, 1/4 wavelength plus 4 wavelengths, etc. One will also see some effects from 2nd and 3rd harmonics of the main constructive/destructive inference.
And one more comment, because of the phase shifting aspects enhancing edge effects, one can increase detection of Becke line for refractive index matching of isotropic materials (glasses for instances in forensic applications). [This can also be improved with Nomarski DIC under similar principles to an order mag better than typical brightfield]
Tony
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-----Original Message----- X-from: frank.karl-at-degussa.com [mailto:frank.karl-at-degussa.com] Sent: Friday, November 10, 2006 9:04 AM To: ph2-at-sprynet.com
At the risk of creating another bump and run... Phase contrast and dark field are different. Phase contrast converts phase differences between the object of interest and the surrounding material to amplitude difference which is what the human eye is sensitive to. One could think of darkfield as a way of increase detectability.
Using a large phase ring in the condenser is a trick. Just a short cut to get illuminated objects on a dark background.
I refer you to Tobias's excellent explanation.
antoniomiguel.garci a-at-gmail.com To: frank.karl-at-degussa.com cc: 11/10/2006 08:35 AM Subject: [Microscopy] AskAMicroscopist:Phase contrast vs Dark Field Please respond to antoniomiguel.garci a
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Hello
Could someone please explain the difference between phase contrast and dark field microcopy? What optic effects one get with one or the other? I see Frank Karl states in his very nice explanation of dark field sent to the list the following: "One easy short cut is to use a phase contrast scope and select a condenser phase ring larger than the ring in your objective". Can Phase contrast be transformed into darkfield then?
Thank you for any explanation
Antonio M Garcia
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Hi Fred, Off line I am sending you the instructions I wrote for a CM12 TEM. Good luck, Judy Murphy microscopyproducts Stockton, CA
fahayes-at-ucdavis.edu wrote:
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Just to give some clarification on this - the chip in question is a Si ASIC, I guess, mounted on a multilayer board which drives a laser, which sends light down an optical fibre; the board slots into a telecoms module. In fact you can see the board at http://www.bookham.com/common/trans_tunable.cfm, or http://www.bookham.com/documents/datasheets_trans/TL5000.pdf. No idea at the moment what the chip does, whether it's bipolar or CMOS, Al or Cu metallisation etc. etc.. There are 8 lead-free solder bumps and there doesn't appear to be any epoxy underfill; the board is expendable. Usually I work on the optoelectronic components produced at the fab here, more recently the miniature optical bench that surrounds it inside a 'gold box', but as we move more into systems rather than just components, things are getting more varied. The failure analysis request at the moment is just "we know this chip has stopped working, what's wrong with it?". I don't think we have the kit here to test the chip's functionality off the board, so if there's nothing obvious like a cracked solder bump or an obvious electrical overstress event I guess it will get pushed back to the PCB supplier.
Some very useful suggestions which I like the sound of: Infra-red through the back of the chip, thanks to Peter Tomic, since this is non-destructive and simple for me to do. Also fuming nitric/sulphuric to get rid of the PCB, thanks Valery Ray. I've already done real-time radiography to look for cracks in the solder and SEM looking around the edges. And perhaps even more important than any technique, knowing some of the right questions to ask about the chip which will make it a lot easier to get a meaningful result.
Thanks to all for a great response to my question,
-----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: 10 November 2006 18:52 To: Richard Beanland
This can be (and usually is) a tricky proposition. It would help knowing a few details about your situation before plunging in to strategies.
First off, why do you need to do failure analysis on this IC? This is not meant to sound flippant. If you are really having to do FA on this IC, depending on its complexity, it can be a huge job and even bigger if you are not set up to do this sort of work on a more routine basis. Even so, different types of ICs tend to need different methods of FA. Based on un-installed identical ICs, what type of metal interconnects are used (Al or Cu), how many layers, ILD type and what method of planarization? Is this flip chip on a supporting substrate or flipped directly onto the main PCB? Is there any conformal coating on the board/IC?
What part number is the IC? This will tell what kind of base package it is--plastic or ceramic and the node size of the IC and if it is Pb-free or not. What kind of PCB is the part on? Is it multi-layer FR4 or ? What is the requirement for retaining the main PCB--i.e., can it be destroyed? Once removed from the main PCB, are you going to re-bump the package and do electrical testing? This would be a first look step to test the IC. The problem with this is that depending on the construction of the whole part, the die and/or package could be destroyed such that the whole thing would no longer work electrically.
gary g.
At 04:53 AM 11/10/2006, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Fri Nov 10 12:50:38 2006 11, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kAAIobdZ024534 11, 20 -- for {microscopy-at-microscopy.com} ; Fri, 10 Nov 2006 12:50:38 -0600 11, 20 -- Received: (qmail 6249 invoked from network); 10 Nov 2006 10:50:36 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 6195, pid: 6244, t: 0.1167s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 20 -- by qsmtp3 with SMTP; 10 Nov 2006 10:50:36 -0800 11, 20 -- Message-Id: {7.0.1.0.2.20061110103602.024d4cb8-at-gaugler.com} 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 11, 20 -- Date: Fri, 10 Nov 2006 10:50:31 -0800 11, 20 -- To: richard.beanland-at-bookham.com 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] Failure analysis;flip-chip dies 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200611101253.kAACrPpa001481-at-ns.microscopy.com} 11, 20 -- References: {200611101253.kAACrPpa001481-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
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==============================Original Headers============================== 27, 31 -- From richard.beanland-at-bookham.com Mon Nov 13 04:42:29 2006 27, 31 -- Received: from mail80.messagelabs.com (mail80.messagelabs.com [195.245.230.163]) 27, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kADAgSh9030669 27, 31 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 04:42:29 -0600 27, 31 -- X-VirusChecked: Checked 27, 31 -- X-Env-Sender: richard.beanland-at-bookham.com 27, 31 -- X-Msg-Ref: server-10.tower-80.messagelabs.com!1163414545!49942741!1 27, 31 -- X-StarScan-Version: 5.5.10.7; banners=bookham.com,-,- 27, 31 -- X-Originating-IP: [213.249.209.179] 27, 31 -- Received: (qmail 9317 invoked from network); 13 Nov 2006 10:42:25 -0000 27, 31 -- Received: from unknown (HELO cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 27, 31 -- by server-10.tower-80.messagelabs.com with SMTP; 13 Nov 2006 10:42:25 -0000 27, 31 -- Received: from cas-smx-02.BOOKHAM.ENTERPRISE.PRI ([10.4.0.223]) by cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI with Microsoft SMTPSVC(6.0.3790.1830); 27, 31 -- Mon, 13 Nov 2006 10:46:33 +0000 27, 31 -- Content-class: urn:content-classes:message 27, 31 -- MIME-Version: 1.0 27, 31 -- Content-Type: text/plain; 27, 31 -- charset="us-ascii" 27, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 27, 31 -- Subject: RE: [Microscopy] Failure analysis;flip-chip dies 27, 31 -- Date: Mon, 13 Nov 2006 10:46:34 -0000 27, 31 -- Message-ID: {9645D3E33E4C6548B12A7B25F611533E243BCC-at-cas-smx-02.BOOKHAM.ENTERPRISE.PRI} 27, 31 -- X-MS-Has-Attach: 27, 31 -- X-MS-TNEF-Correlator: 27, 31 -- Thread-Topic: [Microscopy] Failure analysis;flip-chip dies 27, 31 -- Thread-Index: AccE+dRd2iIwlm4JQYiPjr78y7W6JwCEm24w 27, 31 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 27, 31 -- To: {microscopy-at-microscopy.com} 27, 31 -- X-OriginalArrivalTime: 13 Nov 2006 10:46:33.0171 (UTC) FILETIME=[FBFA6230:01C70710] 27, 31 -- Content-Transfer-Encoding: 8bit 27, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kADAgSh9030669 ==============================End of - Headers==============================
I manage a multi-user facility and are looking into on-line scheduling programs. I'd appreciate your input as to what's out there and your experiences.
Thanks in advance,
Kathy Troughton Senior Research Associate University of Tennessee, Memphis Memphis, TN 901-448-5976 ktrou-at-nb.utmem.edu
==============================Original Headers============================== 5, 26 -- From ktrou-at-nb.utmem.edu Mon Nov 13 08:32:43 2006 5, 26 -- Received: from mail2.utmem.edu (mail2.utmem.edu [132.192.6.164]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kADEWhlE014007 5, 26 -- for {Microscopy-at-Microscopy.Com} ; Mon, 13 Nov 2006 08:32:43 -0600 5, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 5, 26 -- by mail2.utmem.edu (SMTP Daemon) with ESMTP id 05DD734080 5, 26 -- for {Microscopy-at-Microscopy.Com} ; Mon, 13 Nov 2006 08:32:43 -0600 (CST) 5, 26 -- Received: from mail2.utmem.edu ([127.0.0.1]) 5, 26 -- by localhost (mail2.utmem.edu [127.0.0.1]) (amavisd-new, port 10025) 5, 26 -- with ESMTP id 21160-12 for {Microscopy-at-Microscopy.Com} ; 5, 26 -- Mon, 13 Nov 2006 08:32:42 -0600 (CST) 5, 26 -- Received: from nb.utmem.edu (nb.utmem.edu [132.192.43.15]) 5, 26 -- by mail2.utmem.edu (SMTP Daemon) with SMTP id 267B3340D5 5, 26 -- for {Microscopy-at-Microscopy.Com} ; Mon, 13 Nov 2006 08:32:42 -0600 (CST) 5, 26 -- Received: from [132.192.47.92] (b11701.utmem.edu) by nb.utmem.edu (5.0/SMI-SVR4) 5, 26 -- id AA20804; Mon, 13 Nov 06 07:24:07 CST 5, 26 -- Mime-Version: 1.0 (Apple Message framework v752.2) 5, 26 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 26 -- Message-Id: {E59D7F8E-054F-4801-9925-427F07728CBF-at-nb.utmem.edu} 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- From: Kathy Troughton {ktrou-at-nb.utmem.edu} 5, 26 -- Subject: Scheduling programs 5, 26 -- Date: Mon, 13 Nov 2006 08:32:38 -0600 5, 26 -- To: Microscopy-at-Microscopy.Com 5, 26 -- X-Mailer: Apple Mail (2.752.2) 5, 26 -- X-Virus-Scanned: by amavisd-new at utmem.edu ==============================End of - Headers==============================
I have designed a dedicated software for scientific multi-user facilities (Facility Online Manager). The detailed features of this software package is discussed in this month's issue of Microscopy Today (November 2006). This software has been used at Northwestern for 3+ years and has been installed in a couple of other institutions.
Take a look here: http://www.fom.northwestern.edu/
Please drop me email if you need demo accounts to the server.
Shuyou _____________________________ Shuyou Li, Ph.D. NUANCE Center Northwestern University 2220 Campus Drive, 1161 Cook Hall Evanston, IL 60208-3108, USA Ph: (847) 491-6723 Fax: (847) 467-6573 Email: syli-at-northwestern.edu http://www.nuance.northwestern.edu/
-----Original Message----- X-from: ktrou-at-nb.utmem.edu [mailto:ktrou-at-nb.utmem.edu] Sent: Monday, November 13, 2006 8:38 AM To: syli-at-northwestern.edu
Hello everyone,
I manage a multi-user facility and are looking into on-line scheduling programs. I'd appreciate your input as to what's out there and your experiences.
Thanks in advance,
Kathy Troughton Senior Research Associate University of Tennessee, Memphis Memphis, TN 901-448-5976 ktrou-at-nb.utmem.edu
==============================Original Headers============================== 5, 26 -- From ktrou-at-nb.utmem.edu Mon Nov 13 08:32:43 2006 5, 26 -- Received: from mail2.utmem.edu (mail2.utmem.edu [132.192.6.164]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kADEWhlE014007 5, 26 -- for {Microscopy-at-Microscopy.Com} ; Mon, 13 Nov 2006 08:32:43 -0600 5, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 5, 26 -- by mail2.utmem.edu (SMTP Daemon) with ESMTP id 05DD734080 5, 26 -- for {Microscopy-at-Microscopy.Com} ; Mon, 13 Nov 2006 08:32:43 -0600 (CST) 5, 26 -- Received: from mail2.utmem.edu ([127.0.0.1]) 5, 26 -- by localhost (mail2.utmem.edu [127.0.0.1]) (amavisd-new, port 10025) 5, 26 -- with ESMTP id 21160-12 for {Microscopy-at-Microscopy.Com} ; 5, 26 -- Mon, 13 Nov 2006 08:32:42 -0600 (CST) 5, 26 -- Received: from nb.utmem.edu (nb.utmem.edu [132.192.43.15]) 5, 26 -- by mail2.utmem.edu (SMTP Daemon) with SMTP id 267B3340D5 5, 26 -- for {Microscopy-at-Microscopy.Com} ; Mon, 13 Nov 2006 08:32:42 -0600 (CST) 5, 26 -- Received: from [132.192.47.92] (b11701.utmem.edu) by nb.utmem.edu (5.0/SMI-SVR4) 5, 26 -- id AA20804; Mon, 13 Nov 06 07:24:07 CST 5, 26 -- Mime-Version: 1.0 (Apple Message framework v752.2) 5, 26 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 26 -- Message-Id: {E59D7F8E-054F-4801-9925-427F07728CBF-at-nb.utmem.edu} 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- From: Kathy Troughton {ktrou-at-nb.utmem.edu} 5, 26 -- Subject: Scheduling programs 5, 26 -- Date: Mon, 13 Nov 2006 08:32:38 -0600 5, 26 -- To: Microscopy-at-Microscopy.Com 5, 26 -- X-Mailer: Apple Mail (2.752.2) 5, 26 -- X-Virus-Scanned: by amavisd-new at utmem.edu ==============================End of - Headers==============================
==============================Original Headers============================== 18, 20 -- From syli-at-northwestern.edu Mon Nov 13 12:25:01 2006 18, 20 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57]) 18, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kADIP1JV007199 18, 20 -- for {Microscopy-at-Microscopy.Com} ; Mon, 13 Nov 2006 12:25:01 -0600 18, 20 -- Received: from prep (orion.ms.northwestern.edu [129.105.37.66]) 18, 20 -- by merle.it.northwestern.edu (Postfix) with ESMTP id DCEFF2F05F; 18, 20 -- Mon, 13 Nov 2006 12:25:00 -0600 (CST) 18, 20 -- From: "Shuyou Li" {syli-at-northwestern.edu} 18, 20 -- To: {ktrou-at-nb.utmem.edu} , {Microscopy-at-Microscopy.Com} 18, 20 -- Subject: RE: [Microscopy] Scheduling programs 18, 20 -- Date: Mon, 13 Nov 2006 12:24:57 -0600 18, 20 -- Message-ID: {002a01c70751$05bc4b40$42256981-at-prep} 18, 20 -- MIME-Version: 1.0 18, 20 -- Content-Type: text/plain; 18, 20 -- charset="us-ascii" 18, 20 -- Content-Transfer-Encoding: 7bit 18, 20 -- X-Mailer: Microsoft Office Outlook 11 18, 20 -- Thread-Index: AccHMY1f+ngMqTFFSuywWWiDU87xXgAHcHTw 18, 20 -- In-Reply-To: {200611131438.kADEc20u021327-at-ns.microscopy.com} 18, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 ==============================End of - Headers==============================
I have a problem with PIPS (Gatan) in our lab. The samples (including metal and semiconductors) are not milled at all (like 10 hours at voltage 4.5). We just cleaned the penning gauge and two guns. The vacuum and current looks common. I wonder someone can give me suggestion what is the problem.
Thanks.
Jiaming
==============================Original Headers============================== 5, 16 -- From zhangj16-at-msu.edu Mon Nov 13 12:29:40 2006 5, 16 -- Received: from egr.msu.edu (jeeves.egr.msu.edu [35.9.37.127]) 5, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kADITeG3013018 5, 16 -- for {Microscopy-at-Microscopy.Com} ; Mon, 13 Nov 2006 12:29:40 -0600 5, 16 -- Received: from [127.0.0.1] (muran.dhcp.egr.msu.edu [35.9.138.199]) 5, 16 -- by egr.msu.edu (8.13.7/8.13.4) with ESMTP id kADITdGZ007932 5, 16 -- for {Microscopy-at-Microscopy.Com} ; Mon, 13 Nov 2006 13:29:41 -0500 (EST) 5, 16 -- Message-ID: {4558B992.6030803-at-msu.edu} 5, 16 -- Date: Mon, 13 Nov 2006 13:29:38 -0500 5, 16 -- From: Jiaming Zhang {zhangj16-at-msu.edu} 5, 16 -- User-Agent: Thunderbird 1.5.0.8 (Windows/20061025) 5, 16 -- MIME-Version: 1.0 5, 16 -- To: Microscopy-at-Microscopy.Com 5, 16 -- Subject: One problem with Ion milling 5, 16 -- Content-Type: text/plain; charset=GB2312 5, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I need to prepare a self supporting cross section of a multilayer film on a 1" silicon wafer. Problem is the material cannot be heated above room temperature. So as far as I know, I need to use something other than G-1 to prepare the sandwich and something other than crystalbond to do the polishing/dimpling. My first thought is M-bond 610 for the sandwich, I've heard this will cure overnight at RT? And superglue for the polishing, although this will require soaking off between switching sides. M-bond 610 is not soluble in acetone, right?
Any other ideas? Commercial responses are welcome.
The sample will be ion-milled at LN2 temperatures after the polishing/dimpling steps.
Thanks, Leslie _____________________ Leslie Krupp IBM Almaden Research 650 Harry Road, K19/D1 San Jose, CA 95120-6099 (408) 927-3856
==============================Original Headers============================== 5, 27 -- From lkrupp-at-us.ibm.com Mon Nov 13 13:37:14 2006 5, 27 -- Received: from e6.ny.us.ibm.com (e6.ny.us.ibm.com [32.97.182.146]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kADJbEPS029873 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 13:37:14 -0600 5, 27 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234]) 5, 27 -- by e6.ny.us.ibm.com (8.13.8/8.12.11) with ESMTP id kADJbXNw029806 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 14:37:33 -0500 5, 27 -- Received: from d01av02.pok.ibm.com (d01av02.pok.ibm.com [9.56.224.216]) 5, 27 -- by d01relay02.pok.ibm.com (8.13.6/8.13.6/NCO v8.1.1) with ESMTP id kADJbDu5181762 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 14:37:13 -0500 5, 27 -- Received: from d01av02.pok.ibm.com (loopback [127.0.0.1]) 5, 27 -- by d01av02.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id kADJbD9N006725 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 14:37:13 -0500 5, 27 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) 5, 27 -- by d01av02.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id kADJbD4q006713 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 14:37:13 -0500 5, 27 -- To: microscopy-at-microscopy.com 5, 27 -- MIME-Version: 1.0 5, 27 -- Subject: TEM sample prep: no heat adhesives required 5, 27 -- X-Mailer: Lotus Notes Release 7.0 HF277 June 21, 2006 5, 27 -- Message-ID: {OF98894D7C.38D025D7-ON85257225.006B12FC-88257225.006BC206-at-us.ibm.com} 5, 27 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} 5, 27 -- Date: Mon, 13 Nov 2006 11:36:31 -0800 5, 27 -- X-MIMETrack: Serialize by Router on D01ML604/01/M/IBM(Release 7.0.2HF32 | October 17, 2006) at 5, 27 -- 11/13/2006 14:37:12, 5, 27 -- Serialize complete at 11/13/2006 14:37:12 5, 27 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
I understand how and why to perform the diopter adjustment on the eyepieces of a light microscope but I must admit I don't get why this is called a "diopter adjustment". Can someone explain this terminology to me.
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
Since the IC is an 8-connection IC, it is most likely not far from a "trivial" job to analyze it. First off, I think it is not a flip chip but rather a BGA. This is quite a different matter and makes analysis easier. If I am right, the die is on a lead frame and wire bonded to an internal substrate that is then solder bumped. Since the main board is expendable, then just cut out the bad IC (main PCB and all). Then heat to about 125F and cleave it off of the main PCB. I assume that the device is small in size. the other option is to grind off the main PCB. However, if it is assembled as I suspect, the circuit side of the IC is up, not down.
A quick x-ray of the device will show how it is constructed. A side x-ray will reveal the cross section detail of the die and package. From there, the plastic encapsulation can be removed with hot sulfuric or SF6 + O2 plasma. Alternatively, if the die is small, a focused ion beam can selectively expose the die. With an exposed die, reverse engineering can take place and then analyze areas for FA.
If the device is simply not connected well to the main PCB, then C-SAM analysis will show this.
If you are not into FA and reverse engineering, the tools to do this vastly exceed the value of knowing why the part failed, IMO. I suspect that if you return the module to the vendor, they will just say that it failed and offer a replacement module. If the failure happens frequently, I would still revert this issue to the module maker. In all likelihood, they designed the ASIC.
A close up pix of the device would be helpful.
gary g.
At 02:45 AM 11/13/2006, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 16, 20 -- From gary-at-gaugler.com Mon Nov 13 15:28:28 2006 16, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 16, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kADLSSq7020755 16, 20 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 15:28:28 -0600 16, 20 -- Received: (qmail 28958 invoked from network); 13 Nov 2006 13:28:22 -0800 16, 20 -- Received: by simscan 1.1.0 ppid: 28935, pid: 28956, t: 0.3319s 16, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 16, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 16, 20 -- by qsmtp1 with SMTP; 13 Nov 2006 13:28:21 -0800 16, 20 -- Message-Id: {7.0.1.0.2.20061113131511.025c66f0-at-gaugler.com} 16, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 16, 20 -- Date: Mon, 13 Nov 2006 13:28:26 -0800 16, 20 -- To: richard.beanland-at-bookham.com 16, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 16, 20 -- Subject: Re: [Microscopy] RE: Failure analysis;flip-chip dies 16, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 16, 20 -- In-Reply-To: {200611131045.kADAj5Jb001196-at-ns.microscopy.com} 16, 20 -- References: {200611131045.kADAj5Jb001196-at-ns.microscopy.com} 16, 20 -- Mime-Version: 1.0 16, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
On Nov 13, 2006, at 12:58 PM, phillipst-at-missouri.edu wrote:
} I understand how and why to perform the diopter adjustment on the } eyepieces } of a light microscope but I must admit I don't get why this is called a } "diopter adjustment". Can someone explain this terminology to me. } Dear Thomas, A diopter is an inverse meter. (I think; the Handbook of Chemistry and Physics does not have the word in its glossary.) Lens power is measured in diopters, so the adjustment is just that power needed to have the eye pieces focus the image onto your retina. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Mon Nov 13 16:03:39 2006 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kADM3d9i032118 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 13 Nov 2006 16:03:39 -0600 4, 22 -- Received: from water-dog.caltech.edu (water-dog [192.168.1.26]) 4, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 9D0202EEF1 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 13 Nov 2006 14:03:38 -0800 (PST) 4, 22 -- Received: from [192.168.159.233] (jpix-01.caltech.edu [131.215.2.133]) 4, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id 304D43548F 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 13 Nov 2006 14:03:32 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v624) 4, 22 -- In-Reply-To: {200611132058.kADKwdpk009817-at-ns.microscopy.com} 4, 22 -- References: {200611132058.kADKwdpk009817-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {8d1af803286af031668ed3568704015c-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] diopter adjustment 4, 22 -- Date: Mon, 13 Nov 2006 14:08:55 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.624) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.3.3 ==============================End of - Headers==============================
I understand a diopter is the inverse of the focal length in meters (1/meter). I understand how a 2 diopter lens can combined with a 0.5 diopter lens to get a 2.5 diopter effect. but when you "focus" an eyepiece, aren't you simply raising or lowering the height of the ocular? I don't see how this is a diopter adjustment. Tom
At 04:04 PM 11/13/06, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
I assume that you have checked, and tuned, the gun alignment with the fluorescent screen stub and made sure your flow rates are optimised (both of these are in the manual). Another possible one is if you are using a one sided milling stub and both your guns are angled to come from underneath, sounds silly but it can happen!
Matt
} Hi Everyone } } I have a problem with PIPS (Gatan) in our lab. The samples (including } metal and semiconductors) are not milled at all (like 10 hours at } voltage 4.5). We just cleaned the penning gauge and two guns. The vacuum } and current looks common. I wonder someone can give me suggestion what } is the problem. } } Thanks. } } Jiaming
-- Dr M.Weyland, Electron Microscopist
Monash Centre for Electron Microscopy Monash University Clayton Campus VIC 3800 Australia
==============================Original Headers============================== 7, 26 -- From mw275-at-cornell.edu Mon Nov 13 16:31:41 2006 7, 26 -- Received: from ALPHA1.ITS.MONASH.EDU.AU (alpha1.its.monash.edu.au [130.194.1.1]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kADMVema021424 7, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 16:31:41 -0600 7, 26 -- Received: from larry.its.monash.edu.au ([130.194.13.82]) 7, 26 -- by vaxc.its.monash.edu.au (PMDF V6.1 #31276) 7, 26 -- with ESMTP id {01M9JQ18FVWI91WNUK-at-vaxc.its.monash.edu.au} for 7, 26 -- Microscopy-at-microscopy.com; Tue, 14 Nov 2006 09:31:35 +1100 7, 26 -- Received: from larry.its.monash.edu.au (localhost.localdomain [127.0.0.1]) 7, 26 -- by localhost (Postfix) with ESMTP id 1D39480002 for 7, 26 -- {Microscopy-at-microscopy.com} ; Tue, 14 Nov 2006 09:31:35 +1100 (EST) 7, 26 -- Received: from [130.194.139.201] 7, 26 -- (dyn-130-194-139-201.eng.monash.edu.au [130.194.139.201]) 7, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 7, 26 -- (No client certificate requested) (Authenticated sender: mweyland) 7, 26 -- by larry.its.monash.edu.au (Postfix) with ESMTP id D884C3C006 for 7, 26 -- {Microscopy-at-microscopy.com} ; Tue, 14 Nov 2006 09:31:34 +1100 (EST) 7, 26 -- Date: Tue, 14 Nov 2006 09:31:40 +1100 7, 26 -- From: Matthew Weyland {mw275-at-cornell.edu} 7, 26 -- Subject: Re:[Microscopy] One problem with Ion milling 7, 26 -- To: Microscopy-at-microscopy.com 7, 26 -- Message-id: {4558F24C.6000605-at-cornell.edu} 7, 26 -- MIME-version: 1.0 7, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 7, 26 -- Content-transfer-encoding: 7BIT 7, 26 -- User-Agent: Thunderbird 1.5.0.8 (Windows/20061025) ==============================End of - Headers==============================
We use DEVCON 5 minute epoxy to glue face-to-face, then use crazy bond (same as superglue, cyanoacrylate-base) for temporary fixture. Both gel-type or liquid type crazy bond worked fine for us. Acetone can be used to dissolve the bond. One word for DEVCON, it softens a little bit in Acetone, so don't put the ground piece too long time in acetone. M-bond will not be strong enough if it is not heated. Some others use UV-curing bonds as permanent gluing for some optical films and substrates. Good luck!
Young-Woon Kim Seoul National University School of Materials Science and Engineering Tel) +82-2-880-7977 Fax) +82-2-883-8197 e-mail) youngwk-at-snu.ac.kr
-----Original Message----- X-from: lkrupp-at-us.ibm.com [mailto:lkrupp-at-us.ibm.com] Sent: Tuesday, November 14, 2006 4:41 AM To: youngwk-at-snu.ac.kr
Hello-
I need to prepare a self supporting cross section of a multilayer film on a 1" silicon wafer. Problem is the material cannot be heated above room temperature. So as far as I know, I need to use something other than G-1 to prepare the sandwich and something other than crystalbond to do the polishing/dimpling. My first thought is M-bond 610 for the sandwich, I've heard this will cure overnight at RT? And superglue for the polishing, although this will require soaking off between switching sides. M-bond 610 is not soluble in acetone, right?
Any other ideas? Commercial responses are welcome.
The sample will be ion-milled at LN2 temperatures after the polishing/dimpling steps.
Thanks, Leslie _____________________ Leslie Krupp IBM Almaden Research 650 Harry Road, K19/D1 San Jose, CA 95120-6099 (408) 927-3856
==============================Original Headers============================== 5, 27 -- From lkrupp-at-us.ibm.com Mon Nov 13 13:37:14 2006 5, 27 -- Received: from e6.ny.us.ibm.com (e6.ny.us.ibm.com [32.97.182.146]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kADJbEPS029873 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 13:37:14 -0600 5, 27 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234]) 5, 27 -- by e6.ny.us.ibm.com (8.13.8/8.12.11) with ESMTP id kADJbXNw029806 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 14:37:33 -0500 5, 27 -- Received: from d01av02.pok.ibm.com (d01av02.pok.ibm.com [9.56.224.216]) 5, 27 -- by d01relay02.pok.ibm.com (8.13.6/8.13.6/NCO v8.1.1) with ESMTP id kADJbDu5181762 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 14:37:13 -0500 5, 27 -- Received: from d01av02.pok.ibm.com (loopback [127.0.0.1]) 5, 27 -- by d01av02.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id kADJbD9N006725 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 14:37:13 -0500 5, 27 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) 5, 27 -- by d01av02.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id kADJbD4q006713 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 14:37:13 -0500 5, 27 -- To: microscopy-at-microscopy.com 5, 27 -- MIME-Version: 1.0 5, 27 -- Subject: TEM sample prep: no heat adhesives required 5, 27 -- X-Mailer: Lotus Notes Release 7.0 HF277 June 21, 2006 5, 27 -- Message-ID: {OF98894D7C.38D025D7-ON85257225.006B12FC-88257225.006BC206-at-us.ibm.com} 5, 27 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} 5, 27 -- Date: Mon, 13 Nov 2006 11:36:31 -0800 5, 27 -- X-MIMETrack: Serialize by Router on D01ML604/01/M/IBM(Release 7.0.2HF32 | October 17, 2006) at 5, 27 -- 11/13/2006 14:37:12, 5, 27 -- Serialize complete at 11/13/2006 14:37:12 5, 27 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
==============================Original Headers============================== 20, 28 -- From youngwk-at-snu.ac.kr Mon Nov 13 18:04:46 2006 20, 28 -- Received: from nabi1.snu.ac.kr (nabi1.snu.ac.kr [147.46.100.51]) 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAE04jeD001805 20, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 18:04:45 -0600 20, 28 -- Received: from [147.46.100.31] ([147.46.100.31]) 20, 28 -- by nabi1.snu.ac.kr ([147.46.100.51]) 20, 28 -- with ESMTP id 2006111409:04:44:146893.21813.4849961 20, 28 -- for {Microscopy-at-microscopy.com} ; 20, 28 -- Tue, 14 Nov 2006 09:04:44 +0900 (KST) 20, 28 -- Received: from [147.46.233.36] ([147.46.233.36]) 20, 28 -- by auk1.snu.ac.kr ([147.46.100.31]) 20, 28 -- with ESMTP id 2006111409:04:37:580201.30788.353041328 20, 28 -- for {Microscopy-at-microscopy.com} ; 20, 28 -- Tue, 14 Nov 2006 09:04:37 +0900 (KST) 20, 28 -- From: "Young-Woon Kim" {youngwk-at-snu.ac.kr} 20, 28 -- To: {Microscopy-at-microscopy.com} 20, 28 -- Subject: RE: [Microscopy] TEM sample prep: no heat adhesives required 20, 28 -- Date: Tue, 14 Nov 2006 09:06:45 +0900 20, 28 -- Message-ID: {053501c70780$c58a1e00$24e92e93-at-young323} 20, 28 -- MIME-Version: 1.0 20, 28 -- Content-Type: text/plain; 20, 28 -- charset="us-ascii" 20, 28 -- Content-Transfer-Encoding: 7bit 20, 28 -- X-Mailer: Microsoft Office Outlook 11 20, 28 -- Thread-Index: AccHW5qRP/g/6POhSbiX7AjOtNlbuwAIf5JA 20, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 20, 28 -- X-TERRACE-SPAMMARK: NO (SR:22.71) 20, 28 -- (by Terrace) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both millerpod-at-hotmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: millerpod-at-hotmail.com Name: Andy Miller
Organization: Curtin Uni Western Australia
Title-Subject: [Filtered] phillips xl 30 quad current amplifier PCB
Question: Hi All
We have a problem with our Phillips XL 30.
The quad current amplifier PCB card that slots in the back was overheating and disrupts the scanning beam. We use a fan to cool it until we managed to get a replacement but that too had the same problem, which leads us to believe that it may not be a faulty card but a nearby component that is generating excess heat and in turn overheating the card.
Dose this sound feasible? Has anybody experienced this sort of problem? Could there be another reason aside from the remote chance that we got 2 dud cards?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both t.keller-at-uni-jena.de as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: t.keller-at-uni-jena.de Name: Thomas Keller
Organization: Institute of Materials Science / Friedrich-Schiller-University Jena
Title-Subject: [Filtered] Scheduling System
Question: Dear Kathy,
the Faces Scheduling System works fine:
http://faces.ccrc.uga.edu/
-- --------------------------------------------------- Dr. Thomas Keller Institute of Materials Science and Technology (IMT) Friedrich-Schiller-University Jena L–bdergraben 32 D-07743 Jena Germany Phone: ++ 49 3641 947742 Fax: ++ 49 3641 947732 Mobile: ++ 49 170 1439522 Internet: t.keller-at-uni-jena.de --------------------------------------------------- Visit us at http://www.uni-jena.de/matwi/
"Making Materials Science Work 4 U" ---------------------------------------------------
perhaps just some clarification (arguments are not mine.....):
Definitions for:
DIOPTER: A measure of the optical power of a lens, equal to the reciprocal of its focal length in meters.
DIOPTER ADJUSTER: A mechanism in a light microscope eyepiece for adjusting the position of the eyelens to compensate for differences in dioptric power between the eyes of the observer(s)
DIOPTRIC: Regarding an optical system containing refractive elements
Reference: HEATH Julian P (Ed): Dictionary of Microscopy, (Micr.& Analysis) John WILEY & Sons LTD, ISBN-10:0-470-01199-8 (ISBN-13: 978-0-470-01199-7), 2005 pp 357
Assuming you are talking about adjustment of the ocular(s) according to a /your (refraction) myopy or hypermetropy:
I guess, you won't/don't adjust the } ocular's height { (by raising or lowering the "diopter adjustment ring") but instead "realiter" change the focal length of the ocular lens system to adapt the correct centering of } sample/vision rays { ON your retina instead of their otherwise crossing / centering before (myopy) or behind (metropy) the retinal cells. ==} "Adjust the diopter to suit the observer's eyesight. The method differs according to the eyepiece used. Unless the diopter is adjusted, parfocality will not be maintained when the objective is changed. (When using e.g. WHK 10x eyepieces, the focus is adjusted with the focusing knob[s] whil observing through the right eyepiece. The diopter adjustment ring on the left side is then adjusted for maximum image clarity for the left eye)......slightly different adjustment for "finder"-eyepieces (in photomicroscopy)......" Reference: How to improve Photography through the Microscope, Olympus Optical Co.Ltd. brochure (end of 1980ies; M132E-1191T)
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I understand a diopter is the inverse of the focal length in meters (1/meter). I understand how a 2 diopter lens can be combined with a 0.5 diopter lens to get a 2.5 diopter effect. but when you "focus" an eyepiece, aren't you simply raising or lowering the height of the ocular? I don't see how this is a diopter adjustment. Tom
At 04:04 PM 11/13/06, you wrote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
I would like to use a thermoreversible gel (liquid at 4degree and under) to immobilise a multicellular aggregate to the membrane of a Quantomix capsule. This is then inserted in to a SEM.
Question, what is the internal temperature of a SEM?
Many thanks
==============================Original Headers============================== 6, 28 -- From Sam.Murray-at-port.ac.uk Tue Nov 14 08:20:51 2006 6, 28 -- Received: from betel.iso.port.ac.uk (betel.iso.port.ac.uk [148.197.254.2]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAEEKnfw031061 6, 28 -- for {Microscopy-at-Microscopy.Com} ; Tue, 14 Nov 2006 08:20:50 -0600 6, 28 -- Received: from ls04.nwservers.iso.port.ac.uk ([148.197.251.173]:14268) 6, 28 -- by betel.iso.port.ac.uk with smtp (Exim 4.60) 6, 28 -- (envelope-from {Sam.Murray-at-port.ac.uk} ) 6, 28 -- id 1Gjz9Y-0007Ki-IN 6, 28 -- for Microscopy-at-Microscopy.Com; Tue, 14 Nov 2006 14:20:44 +0000 6, 28 -- Received: from LS04/SpoolDir by ls04.nwservers.iso.port.ac.uk (Mercury 1.48); 6, 28 -- 14 Nov 06 14:20:44 +0000 6, 28 -- Received: from SpoolDir by LS04 (Mercury 1.48); 14 Nov 06 14:20:42 +0000 6, 28 -- From: "Samantha Murray" {Sam.Murray-at-port.ac.uk} 6, 28 -- Organization: University of Portsmouth 6, 28 -- To: Microscopy-at-Microscopy.Com 6, 28 -- Date: Tue, 14 Nov 2006 14:20:42 -0000 6, 28 -- MIME-Version: 1.0 6, 28 -- Subject: [Microscopy] Temperature inside SEM chamber? 6, 28 -- Message-ID: {4559D0BE.15056.1DBA01-at-localhost} 6, 28 -- X-Confirm-Reading-To: "Samantha Murray" {Sam.Murray-at-port.ac.uk} 6, 28 -- X-pmrqc: 1 6, 28 -- Return-receipt-to: "Samantha Murray" {Sam.Murray-at-port.ac.uk} 6, 28 -- Priority: normal 6, 28 -- X-mailer: Pegasus Mail for Windows (v4.12a) 6, 28 -- Content-type: text/plain; charset=US-ASCII 6, 28 -- Content-transfer-encoding: 7BIT 6, 28 -- Content-description: Mail message body 6, 28 -- X-Scan-Signature: 0fa7eb88a48416f360b5a84d0500e04d ==============================End of - Headers==============================
Either something is blocking the beams or, more likely, the beams are not aimed properly. Either problem is easy to diagnose as you can see the beams where they strike the sample if you darken the room. The sample holder should fluoresce by itself or paint a little aquadag on it. The beam aiming adjustments are discussed in the instruction book.
Ron Anderson
zhangj16-at-msu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Everyone } } I have a problem with PIPS (Gatan) in our lab. The samples (including } metal and semiconductors) are not milled at all (like 10 hours at } voltage 4.5). We just cleaned the penning gauge and two guns. The vacuum } and current looks common. I wonder someone can give me suggestion what } is the problem. } } Thanks. } } Jiaming } } } ==============================Original Headers============================== } 5, 16 -- From zhangj16-at-msu.edu Mon Nov 13 12:29:40 2006 } 5, 16 -- Received: from egr.msu.edu (jeeves.egr.msu.edu [35.9.37.127]) } 5, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kADITeG3013018 } 5, 16 -- for {Microscopy-at-Microscopy.Com} ; Mon, 13 Nov 2006 12:29:40 -0600 } 5, 16 -- Received: from [127.0.0.1] (muran.dhcp.egr.msu.edu [35.9.138.199]) } 5, 16 -- by egr.msu.edu (8.13.7/8.13.4) with ESMTP id kADITdGZ007932 } 5, 16 -- for {Microscopy-at-Microscopy.Com} ; Mon, 13 Nov 2006 13:29:41 -0500 (EST) } 5, 16 -- Message-ID: {4558B992.6030803-at-msu.edu} } 5, 16 -- Date: Mon, 13 Nov 2006 13:29:38 -0500 } 5, 16 -- From: Jiaming Zhang {zhangj16-at-msu.edu} } 5, 16 -- User-Agent: Thunderbird 1.5.0.8 (Windows/20061025) } 5, 16 -- MIME-Version: 1.0 } 5, 16 -- To: Microscopy-at-Microscopy.Com } 5, 16 -- Subject: One problem with Ion milling } 5, 16 -- Content-Type: text/plain; charset=GB2312 } 5, 16 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== } } }
==============================Original Headers============================== 5, 19 -- From randerson20-at-tampabay.rr.com Tue Nov 14 08:20:57 2006 5, 19 -- Received: from ms-smtp-02.tampabay.rr.com (ms-smtp-02.tampabay.rr.com [65.32.5.132]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAEEKucm031091 5, 19 -- for {Microscopy-at-Microscopy.Com} ; Tue, 14 Nov 2006 08:20:57 -0600 5, 19 -- Received: from [127.0.0.1] (rrcs-24-73-73-214.se.biz.rr.com [24.73.73.214]) 5, 19 -- by ms-smtp-02.tampabay.rr.com (8.13.6/8.13.6) with ESMTP id kAEEKq3q029407; 5, 19 -- Tue, 14 Nov 2006 09:20:54 -0500 (EST) 5, 19 -- Message-ID: {4559D0C3.1010001-at-tampabay.rr.com} 5, 19 -- Date: Tue, 14 Nov 2006 09:20:51 -0500 5, 19 -- From: Ronald Anderson {randerson20-at-tampabay.rr.com} 5, 19 -- User-Agent: Thunderbird 1.5.0.8 (Windows/20061025) 5, 19 -- MIME-Version: 1.0 5, 19 -- To: zhangj16-at-msu.edu, Listserver {Microscopy-at-Microscopy.Com} 5, 19 -- Subject: Re: [Microscopy] One problem with Ion milling 5, 19 -- References: {200611131829.kADITpAI013410-at-ns.microscopy.com} 5, 19 -- In-Reply-To: {200611131829.kADITpAI013410-at-ns.microscopy.com} 5, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
I'm just getting started with serious optical, SEM, and TEM work, but I _do_ have a bit of experience as an ion source designer (and sufferer), of nearly thirty years. I'm not familiar with the particular machine you are having trouble with, but it most likely uses a Kaufman low-energy-broad-beam ion source. I use them for other things, but the most frequent problem is that the two very delicate grids w/ all the tiny holes in them are shorted out by what is technically called 'crud' in the world of sourcery. There is a voltage potential between the two grids that extracts the ion plasma from within the source. It is easily diagnosed if you look at the current meter on the power supply, and see it is shorted out. The current draw will be essentially infinite, and the supply probably has an automatic cut-out to protect itself. If the grids are shorted, then that portion of the supply won't turn on. The remedy is dislodging the offending piece of 'crud' without just moving the problem to another spot. GENTLY try blowing a bit of nitrogen, (or any dry pure gas), over the face of the front grid into the source. If you are _extremely_ fortunate, it will dislodge the 'crud' and give you an open circuit between the two grids. If it doesn't work on the first try, try blowing through the grid from various angles and orientations. It is worth some patient effort, because taking the source apart is a delicate process, and gloves should _always_ be worn, to keep from contaminating the source with oil from fingerprints. There are probably some tiny, delicate, and very important parts involved, so this shouldn't be undertaken by anyone without considerable ion source experience, or you will probably cause even greater problems. On Tuesday, November 14, 2006, at 09:29AM, {randerson20-at-tampabay.rr.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 2, 22 -- From ionsourcerer-at-mac.com Tue Nov 14 11:52:35 2006 2, 22 -- Received: from smtpout.mac.com (smtpout.mac.com [17.250.248.46]) 2, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAEHqWar025148 2, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 14 Nov 2006 11:52:34 -0600 2, 22 -- Received: from mac.com (webmail040-S [10.13.128.40]) 2, 22 -- by smtpout.mac.com (Xserve/8.12.11/smtpout10/MantshX 4.0) with ESMTP id kAEHqP2G026524 2, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 14 Nov 2006 09:52:25 -0800 (PST) 2, 22 -- Received: from webmail040 (localhost [127.0.0.1]) 2, 22 -- by mac.com (8.13.8/webmail040/MantshX 4.0) with ESMTP id kAEHqOn0024793 2, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 14 Nov 2006 09:52:25 -0800 (PST) 2, 22 -- Date: Tue, 14 Nov 2006 09:52:24 -0800 2, 22 -- From: ionsourcerer {ionsourcerer-at-mac.com} 2, 22 -- To: Microscopy-at-Microscopy.Com 2, 22 -- Message-ID: {02BD3DE7-010E-1000-9762-5B3102984122-Webmail-10010-at-mac.com} 2, 22 -- Subject: Re: [Microscopy] Re: One problem with Ion milling 2, 22 -- MIME-Version: 1.0 2, 22 -- Content-Type: text/plain; charset=ISO-8859-1 2, 22 -- Content-Transfer-Encoding: 7bit 2, 22 -- X-Originating-IP: 72.93.106.133 2, 22 -- Received: from [72.93.106.133] from webmail.mac.com with HTTP; Tue, 14 Nov 2006 09:52:24 -0800 2, 22 -- X-Brightmail-Tracker: AAAAAA== 2, 22 -- X-Brightmail-scanned: yes ==============================End of - Headers==============================
Dear Leslie, When we were waiting for our M-610 to arrive, we used Devon 2-ton epoxy to make cross-section samples. It can be squeezed thin and is stronger than 5-minute epoxy, but takes longer to harden up than 5-minute. I think we left it overnight. Regards, Mary Mager Electron Microscopist Materials Eng. UBC #419 - 6350 Stores Rd. Vancouver, B.C. V6T 1Z4 CANADA Phone: 604-822-5648 Fax: 604-822-3619 email: mager-at-interchange.ubc.ca
-----Original Message----- X-from: lkrupp-at-us.ibm.com [mailto:lkrupp-at-us.ibm.com] Sent: Monday, November 13, 2006 11:43 AM To: mager-at-interchange.ubc.ca
Hello-
I need to prepare a self supporting cross section of a multilayer film on a 1" silicon wafer. Problem is the material cannot be heated above room temperature. So as far as I know, I need to use something other than G-1 to prepare the sandwich and something other than crystalbond to do the polishing/dimpling. My first thought is M-bond 610 for the sandwich, I've heard this will cure overnight at RT? And superglue for the polishing, although this will require soaking off between switching sides. M-bond 610 is not soluble in acetone, right?
Any other ideas? Commercial responses are welcome.
The sample will be ion-milled at LN2 temperatures after the polishing/dimpling steps.
Thanks, Leslie _____________________ Leslie Krupp IBM Almaden Research 650 Harry Road, K19/D1 San Jose, CA 95120-6099 (408) 927-3856
==============================Original Headers============================== 5, 27 -- From lkrupp-at-us.ibm.com Mon Nov 13 13:37:14 2006 5, 27 -- Received: from e6.ny.us.ibm.com (e6.ny.us.ibm.com [32.97.182.146]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kADJbEPS029873 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 13:37:14 -0600 5, 27 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234]) 5, 27 -- by e6.ny.us.ibm.com (8.13.8/8.12.11) with ESMTP id kADJbXNw029806 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 14:37:33 -0500 5, 27 -- Received: from d01av02.pok.ibm.com (d01av02.pok.ibm.com [9.56.224.216]) 5, 27 -- by d01relay02.pok.ibm.com (8.13.6/8.13.6/NCO v8.1.1) with ESMTP id kADJbDu5181762 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 14:37:13 -0500 5, 27 -- Received: from d01av02.pok.ibm.com (loopback [127.0.0.1]) 5, 27 -- by d01av02.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id kADJbD9N006725 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 14:37:13 -0500 5, 27 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) 5, 27 -- by d01av02.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id kADJbD4q006713 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 13 Nov 2006 14:37:13 -0500 5, 27 -- To: microscopy-at-microscopy.com 5, 27 -- MIME-Version: 1.0 5, 27 -- Subject: TEM sample prep: no heat adhesives required 5, 27 -- X-Mailer: Lotus Notes Release 7.0 HF277 June 21, 2006 5, 27 -- Message-ID: {OF98894D7C.38D025D7-ON85257225.006B12FC-88257225.006BC206-at-us.ibm.com} 5, 27 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} 5, 27 -- Date: Mon, 13 Nov 2006 11:36:31 -0800 5, 27 -- X-MIMETrack: Serialize by Router on D01ML604/01/M/IBM(Release 7.0.2HF32 | October 17, 2006) at 5, 27 -- 11/13/2006 14:37:12, 5, 27 -- Serialize complete at 11/13/2006 14:37:12 5, 27 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
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} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America I guess you actually mean the temperature under the beam ..... The temperature in the specimen chamber generally is going to be close to ambient (except for an ESEM, were the sample will be ~5 deg C)
Key things to consider are probe current, scan speed, magnification and thermal conduction path away from the imaged area. In the case of Quantomix capsules, with a FEG-SEM it is relatively easy to punch holes in the membrane with the beam, rather less so with W SEMs.
It is also relatively easy in most SEMs to find conditions which will melt lower temperature thermosetting polymers, so local sample temperatures in the 50 to 100 deg C aren't too difficult to generate, with the right sample.
Basically, I think there are so many variables involved, you're just going to have to try it.
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==============================Original Headers============================== 6, 16 -- From larry-at-celtic.freewire.co.uk Tue Nov 14 15:04:26 2006 6, 16 -- Received: from get.freewire.net (get.freewire.net [86.54.106.202] (may be forged)) 6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAEL4Nue017767 6, 16 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 14 Nov 2006 15:04:26 -0600 6, 16 -- Received: from [194.164.78.253] (modem-rack4-modem253.netkonect.net [194.164.78.253]) 6, 16 -- by get.freewire.net (8.13.6/8.11.6) with ESMTP id kAEL2b3B004597; 6, 16 -- Tue, 14 Nov 2006 21:03:31 GMT 6, 16 -- Mime-Version: 1.0 6, 16 -- Message-Id: {p06210202c17fd7b6ceb4-at-[194.164.78.26]} 6, 16 -- In-Reply-To: {200611141429.kAEETu5l019725-at-ns.microscopy.com} 6, 16 -- References: {200611141429.kAEETu5l019725-at-ns.microscopy.com} 6, 16 -- Date: Tue, 14 Nov 2006 20:38:18 +0000 6, 16 -- To: Sam.Murray-at-port.ac.uk, Microscopy-at-MSA.Microscopy.Com 6, 16 -- From: Larry Stoter {larry-at-celtic.freewire.co.uk} 6, 16 -- Subject: Re: [Microscopy] Temperature inside SEM chamber? 6, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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Email: pbgrover-at-yahoo.com Name: Paul Grover
Organization: Purdue University
Title-Subject: [Filtered] integrity in scientific research
Question: Dear Microscopists,
I find it difficult to understand, based on the responses, both on and = offlist, to the thread on research ethics back in April, that no one has = yet posted to the forum I recently set up (Yahoo group 'honestscience'). = Could this be because of concerns over anonymity? If so, please = contact me or someone else to find out how to post using an alias. = Sorry to waste more listserver space, but since quite a few people said = they would welcome such a forum, I just don't understand why there = hasn't been any activity. Best regards to you all.
Paul Grover, Ph.D. Owner, Grover Roofing and Remodeling and Chief Microscopist and Bottle Washer, Microvista Laboratory
After a computer crash and a restart of the system, I found that the beam alignment was much worse than before. I tried reloading previous alignment settings, but with no effect. The main problem is that when I change the magnification, the beam shifts, especially between the mag 44kx and 55kx. I did once the full alignment procedure, and I don't want to repeat it again (and then it is not necessary). I just need to know how to correct this specific problem, either using the direct alignment or within the alignment section. I tried centering the beam with the multifunction knobs using direct alignment/beam shift, saving the alignment for each magnification but it does not fix the problem. Does anyone have any idea?
Stephane
==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Wed Nov 15 08:53:48 2006 6, 19 -- Received: from web37413.mail.mud.yahoo.com (web37413.mail.mud.yahoo.com [209.191.91.145]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kAFErmx4030210 6, 19 -- for {microscopy-at-microscopy.com} ; Wed, 15 Nov 2006 08:53:48 -0600 6, 19 -- Received: (qmail 98133 invoked by uid 60001); 15 Nov 2006 14:53:48 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 6, 19 -- b=xnjd5drUu6sCGIGWFylc1gLavaxo/Ol50NhMMvmfvACvFxMpxxQVlDWA7ME905Kd2jDnm68OuWCo2xtZQFDDBr13561Rd/6NU9ySYJtuK3OXgwtLSa77s5S4/TedzoeSxVchaXkD9Vd+Ps/Sv4dcLhVjqrCraxEFcoJQTJiOvCQ= ; 6, 19 -- Message-ID: {20061115145348.98131.qmail-at-web37413.mail.mud.yahoo.com} 6, 19 -- Received: from [80.122.101.102] by web37413.mail.mud.yahoo.com via HTTP; Wed, 15 Nov 2006 06:53:48 PST 6, 19 -- Date: Wed, 15 Nov 2006 06:53:48 -0800 (PST) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 -- Subject: alignment with tecnai twin G20 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=ascii 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAFErmx4030210 ==============================End of - Headers==============================
I do not know how FEI tell you to align the lenses but set out below are generic lens alignment procedures - after all FEI produce a TEM so TEM procedures should work!
1. Switch between the two magnifications that are proving to be a problem. 2. Check the lens currents to find which lenses are activated during this change 3 If an intermediate lens look at alignment A if a projector lens look at alignment B.
A
A1 When switching the diffraction spot will flash on the screen. A2 Adjust the intermediate lens such that when switching in either direction the diffraction spot appears in exactly the same area, this may not be on the centre of the screen. A3 Adjust the lens above the problem lens to centre this diffraction spot on the screen. A4 Re check from A2.
B
B1 At the lower magnification align the first projector lens image centre on the centre of the screen. B2 Switch to the higher magnification and centre the second projector image centre on the centre of the screen. B3 Repeat until centres are exactly the same.
Hope this helps?
Steve Chapman Senior Consultant Protrain For electron microscopy consultancy and training world wide Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967 Web www.emcourses.com
----- Original Message ----- X-from: {nizets2-at-yahoo.com} To: {protrain-at-emcourses.com} Sent: Wednesday, November 15, 2006 2:54 PM
Hi,
If you mean "mechanical" adjustments of the lenses: you can't do this on a Tecnai.
Are you sure this shift wasn't always happening?
Regards, Sander
-----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: Wednesday, November 15, 2006 7:27 PM To: Stoks, Sander
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Email: j.janssen-at-nki.nl Name: Hans Janssen
Organization: Netherlands Cancer Institute
Title-Subject: [Filtered] low contrast on uranyl staining of tokoyasu sections
Question: For some time now we have problems with low contrast on our tokoyasu sections for immunoEM. After immune incubation we stain for 5 minutes with uranyloxalate pH 7, wash in H2O, followed by 0.6% uranylacetate in methylcellulose. This used to give a great contrast, but now membranes are hardly visible anymore. We tried solving this by using different brands and batches and higher percentages, but so far it did not get better. Maybe I open up an old discussion, but I am new to the listserver, so sorry in that case. Hope somebody can give some new insights.
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Email: pocohe-at-yahoo.com Name: Yong He
Organization:
Title-Subject: [Filtered] instruction for off-axis electron holography
Question: Dear lister, I am trying to research some magnetic materials by off-axis electron holography, but I am a new hand for using electron holography and still want to try it step by step if I can get one manual for electron holography. I will appreciate you if you can send me one copy by email.
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Email: wa5ekh-at-juno.com Name: charles Jeffrey Day
Question: The company I work for in N. Texas has a surplus E3 Electroscan ESEM, 19= 89. When they have decided to replace it with a new instrument, they qui= t repairing this instrument. The ESEM probably has a vacuum guage board = that needs repair and the EDS(Tracor Noran Voyager,Be Window, 1989) also= has a board issue. =
ALSO...I'll tell you working with water(vapor) detection at 1-20 torr ha= s been a complete surprise, or revelation!, to an older EM operator (mys= elf), who was for years been indoctrinated in "High Vac/High Voltage/LOW= Water Vapor,Low Hydrocarbon" EM technology. There is a great deal more = to this "Environmental EM" than I believe we realize. Not just the abili= ty to image uncoated samples! For instance "STEEM" (my favorite personal= term for Transmission ESEM) techniques for Ultrathin Cross Section imag= ing at 20 KV is very interesting example! Have you seen?? ....images fro= m HRETEM? I'd like to hear more from this community. You can contact me at 817-792-1434 (direct or voice mail). Jeff Day wa5ekh-at-juno.com (Charles Jeffrey Day) (note: I receive no personal profit from this.)
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Email: martini-at-accurel.com Name: Martin Izquierdo
Organization: Accurel
Title-Subject: [Filtered] Entry to Mid Level Dual Beam Operator
Question: Accurel Systems located in Sunnyvale, CA is currently looking for an entry to mid level Dual Beam operator.
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Email: DES-at-CDC.GOV Name: Diane Schwegler-Berry
Organization: NIOSH/CDC
Title-Subject: [Filtered] MSDS
Question: I have an old container of Silver tetraphenylporphin sulfanate and wish to dispose of it. I am looking for a MSDS for this chemical. Does anyone have a copy? Thank you.
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Email: smythen-at-musc.edu Name: Nancy Smythe
Organization: Medical University of South Carolina
Title-Subject: [Filtered] Uranyl Acetate staining
Question: We also had the problem several months ago and not just in my lab but the core facility at the university alse. We found, after much frustration, that the older the UA the better it works. Must be something in the processing of the newer batches that is causing this. Lucky for me I found some in the back of the cabinet that will keep me going for a long time.
We've got 5g of UA here, NOS unopened and dating to 1995, if I am reading the faded ink note correctly. It's free to anyone who can arrange for and pay for shipping.
Ron L
-----Original Message----- X-from: smythen-at-musc.edu [mailto:smythen-at-musc.edu] Sent: Thursday, November 16, 2006 9:29 AM To: lherault-at-bu.edu
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please copy both smythen-at-musc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: smythen-at-musc.edu Name: Nancy Smythe
Organization: Medical University of South Carolina
Title-Subject: [Filtered] Uranyl Acetate staining
Question: We also had the problem several months ago and not just in my lab but the core facility at the university alse. We found, after much frustration, that the older the UA the better it works. Must be something in the processing of the newer batches that is causing this. Lucky for me I found some in the back of the cabinet that will keep me going for a long time.
I found the same product as a zinc salt in Sigma (it is written "tetraphenylprophinE) I don't think silver is more toxic than zinc. Actually the product does not seem to be very dangerous. I don't know why the MSDS by Sigma is in french, but I can translate it for you since french is my mother language. I know that this is no sulfanate, but without CAS number, catalog number or molecular weight this is all I could find.
Disposal of the substance Dissolve or mix the product with a solvant and burn in an appropriate incinerator (appropriate for burning chimicals). Please follow the local rules (this is the sadly usual and helpless answer you ever get from Sigma when you ask how to dispose of a product. They sell them, and don't care what they become afterwards).
Hope this helped.
regards,
Stephane
----- Original Message ---- X-from: "DES-at-CDC.GOV" {DES-at-CDC.GOV} To: nizets2-at-yahoo.com Sent: Thursday, November 16, 2006 3:30:43 PM
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Email: DES-at-CDC.GOV Name: Diane Schwegler-Berry
Organization: NIOSH/CDC
Title-Subject: [Filtered] MSDS
Question: I have an old container of Silver tetraphenylporphin sulfanate and wish to dispose of it. I am looking for a MSDS for this chemical. Does anyone have a copy? Thank you.
unfortunately I don't have an MSDS in my relatively huge collection of....and, as you have perhaps also found, there are only a few article links for the stuff in the web online available, no hint for an MSDS....anyway...
Two suggestions (not based on a real "chemical knowledge" of the chemical, but comparing with substances like silver proteinate....SPI product info sheet states "not hazardous in terms of transportation"):
Perhaps either there would be a possibility of recycling the silver (cf. websource } Tape Data Recovery at Ilford { at http://www.data-recovery-london.com/Tape-Data-Recovery-at-Ilford.html , quoting also Silver-tetraphenylporphinsulfonate....but unfortunately there is no free access to the underlying data collection), or, to talk to your safety officer(s) concerning safe disposal (if you do have "analog"/"ancillary" photographic waste solutions perhaps you would like to add the substance to the hydrous waste solution of fixator ....?....but I not quite sure about it....)....
Hoping there is better help out there.... Good luck and best wishes
Wolfgang MUSS Salzburg-Austria
PS: perhaps you can use information by WIKIPEDIA, to evaluate a possible "toxicity" of the substance, searching e.g. for "porphine" and/or "pyrrole" (in the German Wikipedia I found (translated): Porphine, heterocyclic (contains 4 N-atoms), aromatic chemical substance, consisting of 4 Pyrrol-rings (==} Tetrapyrrol)...the Tetrapyrrol-ring forms the basic skeleton of the Haem, of all porphyrins and of chlorophylls....(so perhaps only "phenyl" is left to be examined for a toxicity.....) Sulfonic acids are strong acids, acids and sulfonates mostly are watersoluble and relativley well miscible with water).....
hope that adds some info valuable for getting rid of your problem.....
------------------------------------------------------------------------ ---- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Question: I have an old container of Silver tetraphenylporphin sulfanate and wish to dispose of it. I am looking for a MSDS for this chemical. Does anyone have a copy? Thank you.
MSDSonline - UCSF MSDS Management System http://www.msdsonline.com/accessaccountsearch/default.aspx?id=1000002
Iowa State University - Database of Material Safety Data Sheets http://www.ehs.iastate.edu/isumsds/ehsmsds.asp
Cornell University - Database of Material Safety Data Sheets http://msds.ehs.cornell.edu/msdssrch.asp
Arkansas State University - Database of Material Safety Data Sheets http://ehc.astate.edu/
Howard Hughes Medical Institute - Laboratory Chemical Safety Summaries http://www.hhmi.org/science/labsafe/lcss/index.html
US Environmental Protection Agency - Envirofacts Warehouse Chemical References Index http://www.epa.gov/enviro/html/emci/chemref/index.html
Micromedex Health Series - Summaries & detailed monographs for drugs, toxicological managements, & more.... http://www.thomsonhc.com/hcs/librarian/PFPUI/MY4CzEP1mg4dfj
Macquarie University - Material Safety Data Sheet Information http://www.chem.mq.edu.au/links.html#safety
University of Akron - Hazardous Chemical Database http://ull.chemistry.uakron.edu/erd/
The Vermont SIRI - Collection of Material Safety Data Sheets http://siri.org/msds/
Oxford University - Database of Material Safety Data Sheets http://physchem.ox.ac.uk/MSDS/
Sigma-Aldrich - Database of Material Safety Data Sheets http://www.sigmaaldrich.com/Area_of_Interest/The_Americas/United_States.html
-- Larry Ackerman, Associate Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
==============================Original Headers============================== 19, 30 -- From Larry.Ackerman-at-ucsf.edu Thu Nov 16 11:51:28 2006 19, 30 -- Received: from emfmcb02.ucsfmedicalcenter.org (EMFMCB02.ucsfmedicalcenter.org [64.54.46.98]) 19, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAGHpRtF032384 19, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Nov 2006 11:51:28 -0600 19, 30 -- Received: from 64.54.128.153 by emfmcb02.ucsfmedicalcenter.org with 19, 30 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.1.0)); 19, 30 -- Thu, 16 Nov 2006 10:03:40 -0800 19, 30 -- X-Server-Uuid: 44B63953-633F-4500-B2DA-A3E478718104 19, 30 -- Received: from [128.218.123.88] ([128.218.123.88]) by 19, 30 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.1830); Thu, 16 Nov 19, 30 -- 2006 09:51:22 -0800 19, 30 -- Message-ID: {455CA513.4020805-at-ucsf.edu} 19, 30 -- Date: Thu, 16 Nov 2006 09:51:15 -0800 19, 30 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 19, 30 -- Reply-to: larry.ackerman-at-ucsf.edu 19, 30 -- Organization: UCSF, NeuroAnatomy 19, 30 -- User-Agent: Mozilla Thunderbird 1.0.7 (Macintosh/20050923) 19, 30 -- X-Accept-Language: en-us, en 19, 30 -- MIME-Version: 1.0 19, 30 -- To: DES-at-CDC.GOV, Microscopy-at-microscopy.com 19, 30 -- Subject: Re: [Microscopy] MSDS 19, 30 -- References: {200611161430.kAGEUH1q010255-at-ns.microscopy.com} 19, 30 -- In-Reply-To: {200611161430.kAGEUH1q010255-at-ns.microscopy.com} 19, 30 -- X-OriginalArrivalTime: 16 Nov 2006 17:51:22.0482 (UTC) 19, 30 -- FILETIME=[D407D520:01C709A7] 19, 30 -- X-WSS-ID: 694278761IO4718182-01-01 19, 30 -- Content-Type: text/plain; 19, 30 -- charset=iso-8859-1; 19, 30 -- format=flowed 19, 30 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Greetings all, Does anyone happen to know what the numerical aperture of the c-mount standard for light microscopy is? Thanks in advance. Best Regards, Karl
-- Karl Garsha Head Applications Scientist Roper Bioscience 3440 E. Brittania Drive Tucson, AZ 85706 Office: 520-547-2704
==============================Original Headers============================== 3, 27 -- From garsha-at-itg.uiuc.edu Thu Nov 16 16:39:36 2006 3, 27 -- Received: from outmail129167.authsmtp.com (outmail129167.authsmtp.com [62.13.129.167]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAGMdY5m016846 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 16 Nov 2006 16:39:36 -0600 3, 27 -- Received: from outmail128183.authsmtp.com (outmail128183.authsmtp.com [62.13.128.183]) 3, 27 -- by punt3.authsmtp.com (8.13.8/8.13.8/Kp) with ESMTP id kAGMdXq2029374 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 16 Nov 2006 22:39:33 GMT 3, 27 -- Received: from [144.22.2.171] (66-162-43-45.static.twtelecom.net [66.162.43.45]) 3, 27 -- (authenticated bits=0) 3, 27 -- by mail.authsmtp.com (8.13.6/8.13.6/Kp) with ESMTP id kAGMdRXZ088059 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 16 Nov 2006 22:39:28 GMT 3, 27 -- Message-ID: {455CE89B.6000601-at-itg.uiuc.edu} 3, 27 -- Date: Thu, 16 Nov 2006 15:39:23 -0700 3, 27 -- From: Karl Garsha {garsha-at-itg.uiuc.edu} 3, 27 -- Reply-To: kgarsha-at-roperscientific.com 3, 27 -- Organization: Roper Scientific 3, 27 -- User-Agent: Thunderbird 1.5.0.8 (Windows/20061025) 3, 27 -- MIME-Version: 1.0 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: NA or F# of C-Mount Standard 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- X-Server-Quench: 51cc26a1-75c3-11db-b4dc-001185d377ca 3, 27 -- X-AuthRoute: OCdyaAgTClZaRR4B CiosDTNPDxgkOxYK DBMeOw5bK0AOTg9W KldyK1tYKloHTlZB SnhYBAkaUVtuJzwz dAhRbQNNYEhEXAxg UkAHRFRMFQNqHxgC GBobTRt3dQBZeDAr FDEfChIzNkRyfUZ4 SwBTFWpIYGYzO2IZ AUFFcAYHcx5Lf0sX dwJ7ACAQYWUGZ3Jl E1RsYDs4KxN2YCNP Ci9GZVUcCW1OOzkh QQxKJikmG0EMXSlb 3, 27 -- X-Authentic-SMTP: 61633138303639.squirrel.dmpriest.net.uk:315/Kp 3, 27 -- X-Report-SPAM: If SPAM / abuse - report it at: http://www.authsmtp.com/abuse 3, 27 -- X-Virus-Status: No virus detected - but ensure you scan with your own anti-virus system! ==============================End of - Headers==============================
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Title-Subject: [Filtered] particle tracking and stage movement
Question: I am trying to find out how to track particles under a microscope, and use the particle co-ordinates to reset the stage position (so that I can follow it over long periods of time). We use metamorph to run our microscope and stage, and ideally, I am looking for a solution using this software. Thanks!
Steven Lee Chief Technologist Electron Microscopy Laboratory Baylor University Medical Center 3500 Gaston Ave Dallas, TX 75246 Ph: 214.820.3302 Fx: 214.820.4110 Em: stevenle-at-baylorhealth.edu
-----Original Message----- X-from: W.Muss-at-salk.at [mailto:W.Muss-at-salk.at] Sent: Thursday, November 16, 2006 10:11 AM To: Lee, Steven
Dear Diane,
unfortunately I don't have an MSDS in my relatively huge collection of....and, as you have perhaps also found, there are only a few article links for the stuff in the web online available, no hint for an MSDS....anyway...
Two suggestions (not based on a real "chemical knowledge" of the chemical, but comparing with substances like silver proteinate....SPI product info
sheet states "not hazardous in terms of transportation"):
Perhaps either there would be a possibility of recycling the silver (cf. websource } Tape Data Recovery at Ilford { at http://www.data-recovery-london.com/Tape-Data-Recovery-at-Ilford.html , quoting also Silver-tetraphenylporphinsulfonate....but unfortunately there is no free access to the underlying data collection), or, to talk to your safety officer(s) concerning safe disposal (if you do have "analog"/"ancillary" photographic waste solutions perhaps you would
like to add the substance to the hydrous waste solution of fixator ....?....but I not quite sure about it....)....
Hoping there is better help out there.... Good luck and best wishes
Wolfgang MUSS Salzburg-Austria
PS: perhaps you can use information by WIKIPEDIA, to evaluate a possible
"toxicity" of the substance, searching e.g. for "porphine" and/or "pyrrole" (in the German Wikipedia I found (translated): Porphine, heterocyclic (contains 4 N-atoms), aromatic chemical substance, consisting of 4 Pyrrol-rings (==} Tetrapyrrol)...the Tetrapyrrol-ring forms the basic skeleton of the Haem, of all porphyrins and of chlorophylls....(so perhaps only "phenyl" is left to be examined for a toxicity.....) Sulfonic acids are strong acids, acids and sulfonates mostly are watersoluble and relativley well miscible with water).....
hope that adds some info valuable for getting rid of your problem.....
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---- This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both DES-at-CDC.GOV as well as the MIcroscopy Listserver ------------------------------------------------------------------------ --- Email: DES-at-CDC.GOV Name: Diane Schwegler-Berry Organization: NIOSH/CDC Title-Subject: [Filtered] MSDS
Question: I have an old container of Silver tetraphenylporphin sulfanate
and wish to dispose of it. I am looking for a MSDS for this chemical. Does anyone have a copy? Thank you.
==============================Original Headers============================== 6, 12 -- From zaluzec-at-microscopy.com Thu Nov 16 08:25:16 2006 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAGEPFBO027426 6, 12 -- for {microscopy-at-microscopy.com} ; Thu, 16 Nov 2006 08:25:16 -0600 6, 12 -- Mime-Version: 1.0 6, 12 -- X-Sender: (Unverified) 6, 12 -- Message-Id: {p0611040bc182253462f9-at-[206.69.208.22]} 6, 12 -- Date: Thu, 16 Nov 2006 08:25:14 -0600 6, 12 -- To: microscopy-at-microscopy.com 6, 12 -- From: DES-at-CDC.GOV (by way of MicroscopyListserver) 6, 12 -- Subject: viaWWW: MSDS Silver tetraphenylporphin 6, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
==============================Original Headers============================== 24, 39 -- From W.Muss-at-salk.at Thu Nov 16 10:03:20 2006 24, 39 -- Received: from hermes.salk.at (hermes.lks.at [193.170.167.9]) 24, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAGG3JAp017738 24, 39 -- for {microscopy-at-microscopy.com} ; Thu, 16 Nov 2006 10:03:19 -0600 24, 39 -- Received: from localhost (localhost [127.0.0.1]) 24, 39 -- by hermes.salk.at (Postfix) with ESMTP id 8F7E7C387E; 24, 39 -- Thu, 16 Nov 2006 17:03:16 +0100 (CET) 24, 39 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 24, 39 -- Received: from hermes.salk.at ([127.0.0.1]) 24, 39 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 24, 39 -- with ESMTP id oaQ9yAAWv++s; Thu, 16 Nov 2006 17:03:16 +0100 (CET) 24, 39 -- Received: from hermes.lks.at (hermes.salk.at [192.168.13.210]) 24, 39 -- by hermes.salk.at (Postfix) with ESMTP id 1878CC387A; 24, 39 -- Thu, 16 Nov 2006 17:03:16 +0100 (CET) 24, 39 -- Received: from localhost (localhost [127.0.0.1]) 24, 39 -- by hermes.lks.at (Postfix) with ESMTP id ED1985A9048; 24, 39 -- Thu, 16 Nov 2006 17:03:15 +0100 (CET) 24, 39 -- Received: from hermes.lks.at ([127.0.0.1]) 24, 39 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024) 24, 39 -- with ESMTP id 92792-06; Thu, 16 Nov 2006 17:03:15 +0100 (CET) 24, 39 -- Received: from c1pa003 (unknown [192.168.42.3]) 24, 39 -- by hermes.lks.at (Postfix) with SMTP id 4BD1D5A9044; 24, 39 -- Thu, 16 Nov 2006 17:03:15 +0100 (CET) 24, 39 -- Received: by localhost with Microsoft MAPI; Thu, 16 Nov 2006 17:03:11 +0100 24, 39 -- Message-ID: {01C709A1.18A1F3C0.W.Muss-at-salk.at} 24, 39 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at} 24, 39 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at} 24, 39 -- To: "'DES-at-CDC.GOV'" {DES-at-CDC.GOV} 24, 39 -- Cc: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 24, 39 -- Subject: [Microscopy] Re: MSDS Silver tetraphenylporphinsulfonate (S-TPPS) 24, 39 -- Date: Thu, 16 Nov 2006 17:03:09 +0100 24, 39 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at} 24, 39 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor 24, 39 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211 24, 39 -- MIME-Version: 1.0 24, 39 -- Content-Type: text/plain; charset="us-ascii" 24, 39 -- Content-Transfer-Encoding: 7bit 24, 39 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at 24, 39 -- X-Scanned-By: SALK-Content-Filter ==============================End of - Headers==============================
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==============================Original Headers============================== 41, 25 -- From StevenLe-at-BaylorHealth.edu Fri Nov 17 06:24:11 2006 41, 25 -- Received: from BHDAEXIMS01.bhcs.pvt (mailhost1.baylorhealth.edu [65.248.93.160]) 41, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAHCOAsc022852 41, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Nov 2006 06:24:11 -0600 41, 25 -- Received: from BHDAEXFE01.bhcs.pvt ([10.5.3.72]) by BHDAEXIMS01.bhcs.pvt with InterScan Messaging Security Suite; Fri, 17 Nov 2006 06:24:10 -0600 41, 25 -- Received: from BHDAEXCH11.bhcs.pvt ([10.5.3.67]) by BHDAEXFE01.bhcs.pvt with Microsoft SMTPSVC(6.0.3790.211); 41, 25 -- Fri, 17 Nov 2006 06:24:11 -0600 41, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 41, 25 -- Content-class: urn:content-classes:message 41, 25 -- MIME-Version: 1.0 41, 25 -- Content-Type: text/plain; 41, 25 -- charset="US-ASCII" 41, 25 -- Subject: RE: [Microscopy] Re: MSDS Silver tetraphenylporphinsulfonate (S-TPPS) 41, 25 -- Date: Fri, 17 Nov 2006 06:24:09 -0600 41, 25 -- Message-ID: {74A8B7A904152141BD5AA2761F5D223401FF9D78-at-BHDAEXCH11.bhcs.pvt} 41, 25 -- In-Reply-To: {200611161611.kAGGB54L027323-at-ns.microscopy.com} 41, 25 -- X-MS-Has-Attach: 41, 25 -- X-MS-TNEF-Correlator: 41, 25 -- Thread-Topic: [Microscopy] Re: MSDS Silver tetraphenylporphinsulfonate (S-TPPS) 41, 25 -- Thread-Index: AccJmeULrNy1zzVGRb+UE6aI/f8hagAqSk3g 41, 25 -- From: "Lee, Steven" {StevenLe-at-BaylorHealth.edu} 41, 25 -- To: {Microscopy-at-microscopy.com} 41, 25 -- X-OriginalArrivalTime: 17 Nov 2006 12:24:11.0967 (UTC) FILETIME=[49BEB4F0:01C70A43] 41, 25 -- Content-Transfer-Encoding: 8bit 41, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAHCOAsc022852 ==============================End of - Headers==============================
------------------------------------------------------------------------ ------------------------------------------------------------------------ ------------------------------------------------------------------------ ---------- The Italian Society for Pure and Applied Biophysics (SIBPA http://sibpa.itc.it/) in collaboration with: Istituto Veneto di Scienze Lettere ed Arti (IVSLA)
proudly announces the
XI SCHOOL OF PURE AND APPLIED BIOPHYSICS Advanced Optical Microscopy Methods in Biophysics
Venice, January 29 to February 2 - 2007, Palazzo Franchetti, the prestigious premises of the Istituto di Scienze Lettere e Arti (IVSLA) (http://www.ivsla.it).
Scientific Coordinators
* Giovanni Giacometti (IVSLA and University of Padova) * Giovanni Felice Azzone (IVSLA and University of Padova) * Alberto Diaspro (Dept. of Physics, University of Genova) * Cesare Usai (Institue of Biophysics, CNR)
Director of the School
* Giorgio M. Giacometti (IVSLA and University of Padova) E-mail: gcometti(at)bio.unipd.it
School overview and program Biophysics is a molecular science rapidly moving to the nanoscale. It seeks to explain biological function in terms of the molecular structures and properties of specific molecules. The size of these molecules varies dramatically, from small fatty acids and sugars (~1 nm = 10-9 m), to macromolecules like proteins (5-10 nm), starches (bigger than 1000 nm), and the enormously elongated DNA molecules. Much effort in biophysics is directed to determining the structure of specific biological molecules and of the larger structures into which they assemble. Some of this effort involves inventing new methods and building new instruments for monitoring these structures, and many of the exciting new developments in optical microscopy, in terms of imaging and manipulation, spectroscopy and visualization, are part of this effort.
The School is co-organized by Alberto Diaspro (Department of Physics, University of Genoa) and Cesare Usai (Institute of Biophysics, National Research Council). Topics of the School will be on: CARS (Coherent Anti Raman Scattering), TIRF (Total Internal Reflection Fluorescence), SHG-THG (Second-Third Harmonic Generation), Correlative microscopy, Multiphoton microscopy, Confocal Microscopy, 3D microscopy, Lifetime Imaging Microscopy, FRET (Forster resonance Enrgy Transfer), FRAP (Fluorescence Recovery After Photobleaching), SIngle molecule imaging, Tissue imaging, Cell imaging, Inverse problems in Optical Microscopy (Computational methods in image recovery), 7D Microscopy (adding new dimensions to x-y-z-t), Nanoscopy, Optical Tweezers, Complementary methods, Fluorescence Spectroscopy.
Provisional list of Lecturers includes:
* Wolfgang Becker (Becker-Hickl, Germany) * Paolo Bianchini (Universitŕ di Genova, Dipartimento di Fisica, Genova, I) * Ranieri Bizzarri (NEST-INFM, Scuola Normale Superiore, Pisa, I) * Rolf Th.Borlinghaus (Leica Microsystems CMS, Mannheim, Germany) * Fred Brakenhoff (University of Amsterdam, Swammerdam Institute for LIfe Sciences, NL) * Carlos Bustamante (UC Berkeley, Department of Molecular and Cellular Biology, CA, USA) * Valentina Caorsi (Universitŕ di Genova, Dipartimento di Fisica, Genova, I) * Giuseppe Chirico (Universitŕ di Milano-Bicocca, Dipartimento di Fisica, Milano, I) * Mario Faretta (IFOM-IEO Campus for Oncogenomics, European Institute of Oncology, Milano, I) * Stefan W. Hell (Max-Planck-Institute for Biophysical Chemistry, Dept. NanoBiophotonics, Goettingen, Germany) * Lucie Kubinova (Institute of Physiology, Academy of Sciences of the Czech Republic, Department of Biomathematics, Prague, Czech Republic) * Manuel Martinez Corral (University of Valencia, Department of Optics, ES) * Davide Mazza (Universitŕ di Genova, Dipartimento di Fisica, Genova, I) * Valentina Mussi (NANOMED, Universitŕ di Genova, I) * Erwin Neher (Max Planck Institute for Biophysical Chemistry, Goettingen, Germany) (to be confirmed) * Dario Parazzoli (IFOM-IEO Campus for Oncogenomics, European Institute of Oncology, Milano, I) * Francesco Pavone (LENS, Universitŕ di Firenze, I) * Tullio Pozzan (University of Padova, Department of Biomedical Sciences, Padova, I) * Franco Quercioli (Istituto dei Sistemi Complessi, CNR, Firenze, I) * Gimmi Ratto (Istituto di Neuroscienze, CNR, Pisa, I) * Peter Saggau (Baylor College of Medicine, Dept. Neuroscience, Houston, TX, USA) * Bruno Samorě (Universitŕ di Bologna, Dipartimento di Biochimica, Bologna, I) * Ilaria Testa (Universitŕ di Genova, Dipartimento di Fisica, Genova, I) * Giuseppe Vicidomini (Universitŕ di Genova, Dipartimento di Informatica e Scienze dell'Informazione, Genova, I) * Tony Wilson (University of Oxford, Department of Engineering Science, UK) * Fred Wouters (The Neuroscience Institute, European Neuroscience Institute, Goettingen, Germany)
MAIN SPONSOR: Leica Microsystems, Germany. SUPPORTING SPONSORS (to be updated): ISS, Urbana, Illinois; Becker and Hickl; OKO-LAB; Coherent; Springer and Verlag; Amici del Festival della Scienza.
REGISTRATION The number of students admitted is restricted to 30. The participation fee is 300 Euros which includes five nights accommodation and attendance at the lessons. The participation fee is reduced to 150 Euros in case the attendant does not need accommodation. The participation fee is reduced to 250 Euros for those registering by 15 December 2006. Applicants are admitted to the School by the Scientific Committee based on the information (short curriculum) provided on the pre-registration form, which must be submitted on line using the following application form. The admittance will be individually communicated via e-mail.
FURTHER DETAILS AND UPDATES http://sibpa.itc.it/ (section Biophysics School) http://www.lambs.it (section Events) ------------------------------------------------------------------------ ------------------------------------------------------------------------ ------------------------------------------------------------------------ ---------------------
--------------------------------------------- [...] Dance with wolves and count the stars, including the unseen...(L.Ferlinghetti, Challenges to young poet, 2001)
--------------------------------------------- Alberto Diaspro,LAMBS-IFOM MicroScoBIO Research Center, Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy facsimile +39-010314218 - voice +39-0103536426/480/309; URL: http://www.lambs.it ----------------------------------------------
==============================Original Headers============================== 24, 19 -- From diaspro-at-fisica.unige.it Fri Nov 17 09:51:34 2006 24, 19 -- Received: from phobos.ge.infm.it (phobos.ge.infm.it [193.205.153.2]) 24, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAHFpWw8005820; 24, 19 -- Fri, 17 Nov 2006 09:51:33 -0600 24, 19 -- Received: from [193.205.153.216] (albypc.ge.infm.it [193.205.153.216]) 24, 19 -- by phobos.ge.infm.it (8.11.6/8.11.0) with ESMTP id kAHGqnZ25981; 24, 19 -- Fri, 17 Nov 2006 17:52:49 +0100 24, 19 -- Mime-Version: 1.0 (Apple Message framework v752.2) 24, 19 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 24, 19 -- Message-Id: {05E62BDA-B9A3-4D0D-9BBD-6CECE6D56512-at-fisica.unige.it} 24, 19 -- Cc: MicroscopyListSpamFilter-at-Microscopy.Com, microscopy-at-Microscopy.Com 24, 19 -- From: diaspro {diaspro-at-fisica.unige.it} 24, 19 -- Subject: School of Biophysics 2007 on Advanced Optical Methods - Venice 29 Jan-2 Feb 2007 24, 19 -- Date: Fri, 17 Nov 2006 16:51:30 +0100 24, 19 -- To: mplsm-users-at-yahoogroups.com, 24, 19 -- Confocal List Microscopy {CONFOCAL-at-LISTSERV.BUFFALO.EDU} 24, 19 -- X-Mailer: Apple Mail (2.752.2) 24, 19 -- Content-Transfer-Encoding: 8bit 24, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAHFpWw8005820 ==============================End of - Headers==============================
An immediate opening exists for a skilled microscopist in Dow's Global Analytical Sciences Laboratory - part of Dow's Corporate R&D function. We are seeking an individual with a passion for characterization science and a desire to both apply this science to complex industrial problems and to advance the technology. A Ph.D. in science is preferred, but an individual with a Masters degree and relevant work experience will also be considered. Working experience in electron microscopy is essential with skills in scanning and transmission electron microscopy, energy dispersive x-ray analysis, electron energy loss spectroscopy, electron diffraction, electron tomography and ultramicrotomy preferred. A background in polymer science is a plus. Excellent written and oral communication skills (English) are essential with an ability to work in a globally-diverse team environment. The position supports new product development activities working from a laboratory with a FEG TEM-STEM, 2 TEMs, a FIBSEM, EPMA, FEGSEM with cryotransfer system, a full complement of scanned probe and light microscopes, SAXS, XPS and TOF-SIMS. Recent degree recipients as well as experienced practitioners are invited to apply for this position. The position is located in either Midland, Michigan or Freeport Texas. Applicants must have the ability to work in the USA.
Dow is a leader in science and technology, providing innovative chemical, plastic and agricultural products and services to many essential consumer markets. With annual sales of $40 billion, Dow serves customers in 175 countries and a wide range of markets that are vital to human progress: food, transportation, health and medicine, personal and home care, and building and construction, among others. Committed to the principles of sustainable development, Dow and its 43,000 employees seek to balance economic, environmental and social responsibilities. References to "Dow" or the "Company" mean The Dow Chemical Company and its consolidated subsidiaries unless otherwise expressly noted.
Please submit curriculum vitae and a list of references to Dr. John Blackson Building 1897 The Dow Chemical Company Midland, MI 48667.
Best Regards,
William A. Heeschen, Ph.D. Microscopy, Digital Imaging The Dow Chemical Company Midland, MI 48667 waheeschen-at-dow.com
==============================Original Headers============================== 8, 22 -- From WAHeeschen-at-dow.com Fri Nov 17 12:36:30 2006 8, 22 -- Received: from mail130.messagelabs.com (mail130.messagelabs.com [216.82.250.163]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kAHIaU1u020117 8, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Nov 2006 12:36:30 -0600 8, 22 -- X-VirusChecked: Checked 8, 22 -- X-Env-Sender: WAHeeschen-at-dow.com 8, 22 -- X-Msg-Ref: server-15.tower-130.messagelabs.com!1163788588!8040606!1 8, 22 -- X-StarScan-Version: 5.5.10.7; banners=-,-,- 8, 22 -- X-Originating-IP: [216.99.65.28] 8, 22 -- Received: (qmail 15936 invoked from network); 17 Nov 2006 18:36:29 -0000 8, 22 -- Received: from mail8.dow.com (HELO mante89.nam.dow.com) (216.99.65.28) 8, 22 -- by server-15.tower-130.messagelabs.com with SMTP; 17 Nov 2006 18:36:29 -0000 8, 22 -- Received: by mante89.nam.dow.com with Internet Mail Service (5.5.2658.3) 8, 22 -- id {W5R7YRAB} ; Fri, 17 Nov 2006 13:36:28 -0500 8, 22 -- Message-ID: {0F0E6B7B4F6AE84CBC5E7AF57057FBA8084E8A-at-USMDLMDOWX022.dow.com} 8, 22 -- From: "Heeschen, Bill (WA)" {WAHeeschen-at-dow.com} 8, 22 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 8, 22 -- Subject: Job Posting from Dow Chemical for a microscopist 8, 22 -- Date: Fri, 17 Nov 2006 13:36:50 -0500 8, 22 -- MIME-Version: 1.0 8, 22 -- X-Mailer: Internet Mail Service (5.5.2658.3) 8, 22 -- Content-Type: text/plain ==============================End of - Headers==============================
The EH&S MSDS requesting system is primarily intended for the use of departments and affiliates of Iowa State University. EH&S reserves the right to refuse off-campus requests at their discretion, and will attempt to contact the requester via email if their request is refused.
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-- Larry Ackerman, Associate Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
==============================Original Headers============================== 13, 28 -- From Larry.Ackerman-at-ucsf.edu Fri Nov 17 12:45:36 2006 13, 28 -- Received: from emfmcb02.ucsfmedicalcenter.org (emfmcb02.ucsfmedicalcenter.org [64.54.46.98]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAHIjZ0A030779 13, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Nov 2006 12:45:36 -0600 13, 28 -- Received: from 64.54.128.153 by emfmcb02.ucsfmedicalcenter.org with 13, 28 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.1.0)); 13, 28 -- Fri, 17 Nov 2006 10:57:48 -0800 13, 28 -- X-Server-Uuid: 44B63953-633F-4500-B2DA-A3E478718104 13, 28 -- Received: from [128.218.123.88] ([128.218.123.88]) by 13, 28 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.1830); Fri, 17 Nov 13, 28 -- 2006 10:45:04 -0800 13, 28 -- Message-ID: {455E032F.5090204-at-ucsf.edu} 13, 28 -- Date: Fri, 17 Nov 2006 10:45:03 -0800 13, 28 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 13, 28 -- Reply-to: larry.ackerman-at-ucsf.edu 13, 28 -- Organization: UCSF, NeuroAnatomy 13, 28 -- User-Agent: Mozilla Thunderbird 1.0.7 (Macintosh/20050923) 13, 28 -- X-Accept-Language: en-us, en 13, 28 -- MIME-Version: 1.0 13, 28 -- To: Microscopy-at-microscopy.com 13, 28 -- Subject: MSDS corrections 13, 28 -- X-OriginalArrivalTime: 17 Nov 2006 18:45:05.0819 (UTC) 13, 28 -- FILETIME=[7FB3C6B0:01C70A78] 13, 28 -- X-WSS-ID: 6940D9A61GO31294-11-01 13, 28 -- Content-Type: text/plain; 13, 28 -- charset=iso-8859-1; 13, 28 -- format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
When exposed to fluorescent illumination, will all fluorescent objects in the field of view bleach at the same extent? ie. will brilliant objects fade less than dim objects?
Thank you, have a nice week-end!
Marie-Claude Bélanger Montréal
_________________________________________________________________ Achetez ce que vous voulez, quand vous voulez sur Sympatico / MSN Magasiner http://magasiner.sympatico.msn.ca/content/shp/?ctId=101,ptnrid=176,ptnrdata=081805
==============================Original Headers============================== 6, 21 -- From mcbelanger6-at-hotmail.com Fri Nov 17 13:02:32 2006 6, 21 -- Received: from bay0-omc3-s5.bay0.hotmail.com (bay0-omc3-s5.bay0.hotmail.com [65.54.246.205]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAHJ2Wsn009317 6, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 17 Nov 2006 13:02:32 -0600 6, 21 -- Received: from hotmail.com ([64.4.17.41]) by bay0-omc3-s5.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.1830); 6, 21 -- Fri, 17 Nov 2006 11:02:31 -0800 6, 21 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 6, 21 -- Fri, 17 Nov 2006 11:02:31 -0800 6, 21 -- Message-ID: {BAY111-F31BD14A9D96DC5B8054BB7E3E80-at-phx.gbl} 6, 21 -- Received: from 64.4.17.200 by by111fd.bay111.hotmail.msn.com with HTTP; 6, 21 -- Fri, 17 Nov 2006 19:02:28 GMT 6, 21 -- X-Originating-IP: [66.46.83.20] 6, 21 -- X-Originating-Email: [mcbelanger6-at-hotmail.com] 6, 21 -- X-Sender: mcbelanger6-at-hotmail.com 6, 21 -- From: =?iso-8859-1?B?TWFyaWUtQ2xhdWRlIELpbGFuZ2Vy?= {mcbelanger6-at-hotmail.com} 6, 21 -- To: Microscopy-at-MSA.Microscopy.Com 6, 21 -- Subject: LM-Photobleaching 6, 21 -- Date: Fri, 17 Nov 2006 19:02:28 +0000 6, 21 -- Mime-Version: 1.0 6, 21 -- Content-Type: text/plain; charset=iso-8859-1; format=flowed 6, 21 -- X-OriginalArrivalTime: 17 Nov 2006 19:02:31.0424 (UTC) FILETIME=[EEEE7000:01C70A7A] ==============================End of - Headers==============================
Particle tracking is inbuilt into MetaMorph Premiere - applications Track Objects and Track Points are under Apps. With track points you manually click on the centre of the point (or the bit you are tracking. If the image is 'calibrated' under MetaMorph the App will give you micron distance travelled, velocity etc when logged to Excel, but to get a fix on the actual position you will need the X,Y co-ordinates from the data output (typically related to say the top left corner of the image and the pixel size - say 1024 x 1024). If you are just tracking within the image this is all you need (but you need to keep the pixel size of the images constant or calibrate/correct the image size). Time-lapse interval just needs to be set so that you can see which particle is which as they move.
If you are tracking beyond the image (using a motorised stage & perhaps raster scanning) then you need to figure in the motorised stage co-ordinates. To set the same 0,0 origin on a motorised stage I always used the same point on an England Finder slide (Graticules.com) but some use the X,Y stops which is probably not as accurate (I'll send my 'England Finder' help guide as well). Raster scans are set to the image size and so are easy to estimate distance across scans. For some odd reason our microscopes were bought without motorised stages [before my time], so I haven't used MetaMorph with a MetaMorph controlled motorised stage but I assume Metamorph can extrapolate the particles X,Y co-ordinates right back to this 0,0 origin on the slide (this may be beyond the Raster/snake scan and you may have to calculate relative to this start point). Track objects is similar etc track points except that this can use thresholding to locate the objects (not always successful so try everything like contrast enhancement (DIC/Phase), autofluorescence, dilution etc.. to get discreet particles). There are bugs in MetaMorph 6 (the version I use) regarding tracking co-ordinates so I'll email you my help pdf's and associated excel spreadsheet discussing this (later versions may have this bug fixed).
When manually clicking on a point in each image be careful of moving slightly back and forward across each subsequent image, as this will clock up a large distance travelled (back and forwards) even if the object hasn't moved at all - a time plot of all the movement X,Y points will demonstrate the object hasn't moved, and a calculation of the distance from the first point to last point will provide the actual distance moved - do this in Excel or MatLab. You may find that MatLab may be useful for calculations once you have the X,Y co-ordinates, but generally I have always got MetaMorph to do everything I need provided I spend a while thinking about it (some aspects of the software are rather poor - like no manual binary image editor for thresholded images - but it always gets the job done and it knocks the spots off simpler ImageJ (for a price) and MatLab (for ease of use and inbuilt image analysis routines). Do email MetaMorph support if you get into problems (or you local rep who will generally be very helpful).
Unfortunate my 5-year contract with the Institute of Opthalmology has just terminated (I'm 'between' jobs at the moment) - so I have lost access to my MetaMorph key, but hopefully my help guides will get you going (note that they are only written with screenshots to remind me what to do, they aren't proof read). Likewise my UCL email address will no doubt fail soon.
If you don't have the Apps (they may not be available under MetaMorph Basic) just use XY co-ordinates from Metamorph object threshold/measurements or perhaps manual count (I think that gives the X,Y co-ordinates if logged to Excel).
Hope this helps.
Keith
MetaMorph http://www.moleculardevices.com
--------------------------------------------
Dr Keith J Morris Manager Imaging Facilties Cell Biology Division The institute of Ophthalmology 11-43 Bath Street London EC1V 9EL
----- Original Message ----- X-from: {sharad-at-post.harvard.edu} To: {keith.morris-at-ucl.ac.uk} Sent: Friday, November 17, 2006 3:30 AM
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Title-Subject: [Filtered] measurement of fluorescence
Question: Occasionally the question of measuring a fluorescence signal inside a yeast cell arises (specifically gfp tagged protein localizing to an organelle). We have a Zeiss Axioskop and use the Axiovision software. Our axiocam takes b/w digital images. The aim to measure at least a hundred cells each from various mutants. Anyone have suggestions or advice?
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Butterflies "can fly because they take themselves lightly"
Nancy Smythe wrote: ==================================== Question: We also had the problem several months ago and not just in my lab but the core facility at the university alse. We found, after much frustration, that the older the UA the better it works. Must be something in the processing of the newer batches that is causing this. Lucky for me I found some in the back of the cabinet that will keep me going for a long time. ==================================== The uranyl acetate used in EM laboratories, does not all come from the same place. The problems that were experienced seem to have been traced to a particular source.
SPI Supplies, and presumably other suppliers, presently have in stock "fresh" product, it is very fast dissolving, and works just fine in the hands of customers who have purchased and used it. I don't think it is correct to say that "older is better" (unless you are comparing some of the "problem" lot material with older but good material.
If someone has had problems with the SPI-Chem brand of uranyl acetate from SPI Supplies, I would like to know about it, especially if such problems have been relatively recent. So far as I know, our "fresh" UA works just fine.
Disclaimer: SPI Supplies is a supplier of uranyl acetate for EM laboratories.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
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==============================Original Headers============================== 10, 26 -- From cgarber-at-2spi.com Mon Nov 20 02:18:51 2006 10, 26 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAK8Ipe5010961 10, 26 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Nov 2006 02:18:51 -0600 10, 26 -- Received: from yourb27fb1c401 (c-71-230-36-118.hsd1.pa.comcast.net [71.230.36.118]) 10, 26 -- (authenticated bits=0) 10, 26 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id kAK8Ii59021853; 10, 26 -- Mon, 20 Nov 2006 03:18:50 -0500 10, 26 -- X-IDV-FirstRcvd: c-71-230-36-118.hsd1.pa.comcast.net [71.230.36.118] 10, 26 -- X-IDV-HELO: yourb27fb1c401 10, 26 -- X-IDV-Authenticated-User: cgarber 10, 26 -- Message-ID: {01a601c70c7c$7f4c3fd0$6401a8c0-at-yourb27fb1c401} 10, 26 -- From: "Garber, Charles A" {cgarber-at-2spi.com} 10, 26 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 10, 26 -- Subject: Uranyl acetate 10, 26 -- Date: Mon, 20 Nov 2006 03:18:43 -0500 10, 26 -- MIME-Version: 1.0 10, 26 -- Content-Type: text/plain; 10, 26 -- format=flowed; 10, 26 -- charset="iso-8859-1"; 10, 26 -- reply-type=original 10, 26 -- Content-Transfer-Encoding: 7bit 10, 26 -- X-Priority: 3 10, 26 -- X-MSMail-Priority: Normal 10, 26 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2869 10, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 ==============================End of - Headers==============================
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A question for those who have an Iridium sputter coater for HR-SEM.
What are your usual sputter conditions for high resolution : target diameter, sample to target distance, current, voltage, gas pressure, runtime ?
Thanks
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
We are in need of a sample holder, single or double tilt, to fit a Philips/FEI EM-420. If anyone has one they are no longer using contact me offline. Thanks, David
****************************** David C. Garrett, Ph.D. Supervisor, Electron Microscopy Dept. Materials Science University of North Texas P.O. Box 305310 Denton, TX 76203-5310 940.565.3964 dgarrett-at-unt.edu ******************************
==============================Original Headers============================== 3, 27 -- From dgarrett-at-unt.edu Mon Nov 20 10:13:18 2006 3, 27 -- Received: from mailhost.unt.edu (mailhost.unt.edu [129.120.188.67]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAKGDIXu026134 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 20 Nov 2006 10:13:18 -0600 3, 27 -- Received: from iatro (localhost.localdomain [127.0.0.1]) 3, 27 -- by mail5 (Postfix) with SMTP id 31CD237B87 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 20 Nov 2006 10:12:47 -0600 (CST) 3, 27 -- X-Spam-Checker-Version: SpamAssassin 3.1.7 (2006-10-05) on 3, 27 -- mail5.ncsmail.private.unt.edu 3, 27 -- X-Spam-Level: 3, 27 -- X-Spam-Status: No, score=-6.0 required=6.0 tests=UNT_LOCAL_FROM 3, 27 -- autolearn=disabled version=3.1.7 3, 27 -- Received: from GWIA.unt.edu (gwia.unt.edu [129.120.221.21]) 3, 27 -- by mailhost.unt.edu (Postfix) with ESMTP id EC9D737BD7 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 20 Nov 2006 10:12:37 -0600 (CST) 3, 27 -- Received: from SMTP-MTA by GWIA.unt.edu 3, 27 -- with Novell_GroupWise; Mon, 20 Nov 2006 10:13:08 -0600 3, 27 -- Message-Id: {45617FAA020000CC0000AB9C-at-GWIA.unt.edu} 3, 27 -- X-Mailer: Novell GroupWise Internet Agent 7.0.1 3, 27 -- Date: Mon, 20 Nov 2006 10:12:58 -0600 3, 27 -- From: "David Garrett" {dgarrett-at-unt.edu} 3, 27 -- To: {microscopy-at-microscopy.com} 3, 27 -- Subject: Need: sample holder Philips/FEI 400 series 3, 27 -- Mime-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=US-ASCII 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Using a Denton Desk IV TSC, the target is 55mm diameter and distance to SEM stub is about 20mm.
Typical conditions are 15mT and 15mA (unknown voltage). Depending on rotation %, 45 seconds works well. Faster rotation seems to require longer time.
gary g.
At 07:45 AM 11/20/2006, you wrote:
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==============================Original Headers============================== 11, 22 -- From gary-at-gaugler.com Mon Nov 20 10:31:10 2006 11, 22 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kAKGV98d004776 11, 22 -- for {microscopy-at-microscopy.com} ; Mon, 20 Nov 2006 10:31:10 -0600 11, 22 -- Received: (qmail 8285 invoked from network); 20 Nov 2006 08:31:09 -0800 11, 22 -- Received: by simscan 1.1.0 ppid: 8252, pid: 8283, t: 0.1647s 11, 22 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 22 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 22 -- by qsmtp3 with SMTP; 20 Nov 2006 08:31:09 -0800 11, 22 -- Message-Id: {7.0.1.0.2.20061120082146.024e0448-at-gaugler.com} 11, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 11, 22 -- Date: Mon, 20 Nov 2006 08:31:02 -0800 11, 22 -- To: jacques.faerber-at-ipcms.u-strasbg.fr 11, 22 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 22 -- Subject: Re: [Microscopy] SEM Ir coating quest. 11, 22 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 22 -- In-Reply-To: {200611201545.kAKFjXDP017585-at-ns.microscopy.com} 11, 22 -- References: {200611201545.kAKFjXDP017585-at-ns.microscopy.com} 11, 22 -- Mime-Version: 1.0 11, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 11, 22 -- Content-Transfer-Encoding: 8bit 11, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAKGV98d004776 ==============================End of - Headers==============================
I'm making up a 5% ammonium molybdate / 1% trehalose w/v negative stain for a Special Project. Is there any trick to this? The solution is staying stubbornly milky so far. Does this clear up after doing a pH adjustment or is it just hard to get into solution?
Thanks!
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 6, 23 -- From TindallR-at-missouri.edu Mon Nov 20 14:47:28 2006 6, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAKKlRTs022628 6, 23 -- for {microscopy-at-microscopy.com} ; Mon, 20 Nov 2006 14:47:28 -0600 6, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 6, 23 -- Mon, 20 Nov 2006 14:47:27 -0600 6, 23 -- x-mimeole: Produced By Microsoft Exchange V6.5 6, 23 -- Content-class: urn:content-classes:message 6, 23 -- MIME-Version: 1.0 6, 23 -- Content-Type: text/plain; 6, 23 -- charset="us-ascii" 6, 23 -- Subject: Neg stain: ammonium molybdate 6, 23 -- Date: Mon, 20 Nov 2006 14:47:27 -0600 6, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68BDA-at-UM-XMAIL08.um.umsystem.edu} 6, 23 -- X-MS-Has-Attach: 6, 23 -- X-MS-TNEF-Correlator: 6, 23 -- Thread-Topic: Neg stain: ammonium molybdate 6, 23 -- Thread-Index: AccM5RWgDOs3TqeNTwO4ZsYvy8qGow== 6, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 6, 23 -- To: {microscopy-at-microscopy.com} 6, 23 -- X-OriginalArrivalTime: 20 Nov 2006 20:47:27.0450 (UTC) FILETIME=[16E503A0:01C70CE5] 6, 23 -- Content-Transfer-Encoding: 8bit 6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAKKlRTs022628 ==============================End of - Headers==============================
Why 5% AM. Way back when, I routinely used a 1% AM. If I remember correctly it did take a little while for the AM to dissolve, maybe overnight????
The sugar may also interfere with the dissolving of the AM, but I have no idea about that aspect.
Best of luck,
Ed
Edward P. Calomeni Director EM Lab - Pathology The Ohio State University M018 Starling Loving Hall 320 W. 10th Ave. Columbus, OH 43210 614-293-5580 (office) 614-293-8806 (lab) edward.calomeni-at-osumc.edu -----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: Monday, November 20, 2006 3:53 PM To: Calomeni, Edward
Dear Listers,
I'm making up a 5% ammonium molybdate / 1% trehalose w/v negative stain for a Special Project. Is there any trick to this? The solution is staying stubbornly milky so far. Does this clear up after doing a pH adjustment or is it just hard to get into solution?
Thanks!
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 6, 23 -- From TindallR-at-missouri.edu Mon Nov 20 14:47:28 2006 6, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAKKlRTs022628 6, 23 -- for {microscopy-at-microscopy.com} ; Mon, 20 Nov 2006 14:47:28 -0600 6, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 6, 23 -- Mon, 20 Nov 2006 14:47:27 -0600 6, 23 -- x-mimeole: Produced By Microsoft Exchange V6.5 6, 23 -- Content-class: urn:content-classes:message 6, 23 -- MIME-Version: 1.0 6, 23 -- Content-Type: text/plain; 6, 23 -- charset="us-ascii" 6, 23 -- Subject: Neg stain: ammonium molybdate 6, 23 -- Date: Mon, 20 Nov 2006 14:47:27 -0600 6, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68BDA-at-UM-XMAIL08.um.umsystem.edu} 6, 23 -- X-MS-Has-Attach: 6, 23 -- X-MS-TNEF-Correlator: 6, 23 -- Thread-Topic: Neg stain: ammonium molybdate 6, 23 -- Thread-Index: AccM5RWgDOs3TqeNTwO4ZsYvy8qGow== 6, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 6, 23 -- To: {microscopy-at-microscopy.com} 6, 23 -- X-OriginalArrivalTime: 20 Nov 2006 20:47:27.0450 (UTC) FILETIME=[16E503A0:01C70CE5] 6, 23 -- Content-Transfer-Encoding: 8bit 6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAKKlRTs022628 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 31 -- From Edward.Calomeni-at-osumc.edu Mon Nov 20 16:28:05 2006 18, 31 -- Received: from pluto.osumc.edu (pluto.osumc.edu [140.254.120.27]) 18, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAKMS47a003233 18, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Nov 2006 16:28:04 -0600 18, 31 -- Received: from localhost (unknown [127.0.0.1]) 18, 31 -- by pfeg01.osumc.edu (Postfix) with ESMTP id ABA5F14103; 18, 31 -- Mon, 20 Nov 2006 17:28:01 -0500 (EST) 18, 31 -- Received: from pfeg01.osumc.edu ([127.0.0.1]) 18, 31 -- by localhost (pfeg01.osumc.edu [127.0.0.1]) (amavisd-new, port 10024) 18, 31 -- with LMTP id 23883-01-66; Mon, 20 Nov 2006 17:28:01 -0500 (EST) 18, 31 -- Received: from msxc01.OSUMC.EDU (msxc01.osumc.edu [10.127.29.33]) 18, 31 -- by pfeg01.osumc.edu (Postfix) with ESMTP id 86C01140F7; 18, 31 -- Mon, 20 Nov 2006 17:28:01 -0500 (EST) 18, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 18, 31 -- Content-class: urn:content-classes:message 18, 31 -- MIME-Version: 1.0 18, 31 -- Content-Type: text/plain; 18, 31 -- charset="us-ascii" 18, 31 -- Subject: RE: [Microscopy] Neg stain: ammonium molybdate 18, 31 -- Date: Mon, 20 Nov 2006 17:28:01 -0500 18, 31 -- Message-ID: {7A541592F9A53A4E86E7A3D5C4C7F68CB4A9BC-at-msxc01.OSUMC.EDU} 18, 31 -- In-Reply-To: {200611202052.kAKKqZxi030051-at-ns.microscopy.com} 18, 31 -- X-MS-Has-Attach: 18, 31 -- X-MS-TNEF-Correlator: 18, 31 -- Thread-Topic: [Microscopy] Neg stain: ammonium molybdate 18, 31 -- Thread-Index: AccM5jQ5erKdqArMRT6NZ/2KK1EGCwADHCHg 18, 31 -- From: "Calomeni, Edward " {Edward.Calomeni-at-osumc.edu} 18, 31 -- To: {TindallR-at-missouri.edu} , {Microscopy-at-microscopy.com} 18, 31 -- X-Virus-Scanned: by amavisd-new at osumc.edu 18, 31 -- Content-Transfer-Encoding: 8bit 18, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAKMS47a003233 ==============================End of - Headers==============================
I've never had a problem with molybdate. The milkiness suggests two things, pH or concentration. Several thoughts which may help.
First, what is the pH you are adjusting to. Ammonium molubdate is naturally weakly acidic. I can adjust the pH to 6.0 readily, but I have found high pH (} 6.0) leads to poor solubility.
Second, the concentration I use is 8.75mM, or just over 1%. This is adjusted so that the number of dense atoms is controled between different stains - eg. the number of Mo atoms able to deflect the beam at 8.75mM is the same as the number of W in 2.5mM PTA or U in any formulation of 60mM uranyl stain (acetate, oxylate, formate, sulfate, etc) You are using 5% - pretty high. Because the stain will dry down, do you really need that high of a concentration.
Third. Never used trehalose, but been tempted to give it a try. Does 1% trehalose go into solution readily. Does it effect the pH of the solution - perhaps raising it above 7.0? Will trehalose go into solution at lower pH? Can you adjust the pH to between 3.0 and 6.0, and if you do, what happens?
Finally, when you mix the two compounds you will effect the saturation point for the two, and lower the relative solubility of each component. Solubility of AmMo is quite high. I don't know the solubility of trehalose, but Harris has used 10% solutions, and it's insoluble in organic solvents, and weakly soluble in alcohol. Perhaps you are affecting the saturation point of the trehalose by mixing with AmMo. I know Harris has done work as you describe, and believe Charles Humphrey at the CDC may have also, but I do not know if there are any side issues.
I have tried mixed stains. Many times they reacted and caused all sorts of precipitates, colloidal milkiness, etc. Maybe they just won't work together under the conditions you are using.
Paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-3926
==============================Original Headers============================== 14, 20 -- From paul_hazelton-at-umanitoba.ca Mon Nov 20 16:36:46 2006 14, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAKMajXK013864 14, 20 -- for {microscopy-at-microscopy.com} ; Mon, 20 Nov 2006 16:36:45 -0600 14, 20 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 14, 20 -- (authenticated bits=0) 14, 20 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id kAKMZM6N005739; 14, 20 -- Mon, 20 Nov 2006 16:35:23 -0600 (CST) 14, 20 -- Message-ID: {45622DA3.8080408-at-umanitoba.ca} 14, 20 -- Date: Mon, 20 Nov 2006 16:35:15 -0600 14, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 14, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 14, 20 -- X-Accept-Language: en-us, en 14, 20 -- MIME-Version: 1.0 14, 20 -- To: TindallR-at-missouri.edu, Microscopy Listserver {microscopy-at-microscopy.com} 14, 20 -- Subject: Re: [Microscopy] Neg stain: ammonium molybdate 14, 20 -- References: {200611202050.kAKKo09W026162-at-ns.microscopy.com} 14, 20 -- In-Reply-To: {200611202050.kAKKo09W026162-at-ns.microscopy.com} 14, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: jensim-at-inbox.com Name: J S
Title-Subject: [Filtered] Kalling's No 2 for duplex stainless steel.
Question: May you explain me the microstructure revealed in duplex stainless steel etched with Kalling's No2? Which phase is the ferrite and which the austenite? Thank you in advance for your time. I am looking forward to receiving a reply from you
This Question was submitted to Ask-A-Microscopist by (jcraft-at-memphis.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, November 20, 2006 at 10:55:06 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both jcraft-at-memphis.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: jcraft-at-memphis.edu Name: jackie williams
I'm puzzled by the interpretation and value of EBIC as specimen current monitor versus simple SE detection.
If a specimen is imaged with E-T SE and then with specimen current monitor (SCM) via stage current monitor, what might the differences be? I see them as a measure of conductivity for SCM whereas the SE is generation of reflected SEs.
Does the nature of reflected SEs directly correlate to the amount of SCM?
What info can be gleaned by using SCM versus SE?
All thoughts invited.
gary g.
==============================Original Headers============================== 8, 17 -- From gary-at-gaugler.com Mon Nov 20 20:32:59 2006 8, 17 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 8, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kAL2WwcS018371 8, 17 -- for {microscopy-at-microscopy.com} ; Mon, 20 Nov 2006 20:32:58 -0600 8, 17 -- Received: (qmail 2638 invoked from network); 20 Nov 2006 18:32:58 -0800 8, 17 -- Received: by simscan 1.1.0 ppid: 2617, pid: 2636, t: 0.0983s 8, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 8, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 8, 17 -- by qsmtp2 with SMTP; 20 Nov 2006 18:32:58 -0800 8, 17 -- Message-Id: {7.0.1.0.2.20061120182645.024cef88-at-gaugler.com} 8, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 8, 17 -- Date: Mon, 20 Nov 2006 18:32:51 -0800 8, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 8, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 8, 17 -- Subject: EBIC vs. SE 8, 17 -- Mime-Version: 1.0 8, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I need to prepare the LED chip for back side SIMS anaysis. SIMS depth profile resolution is significantly degraded if a large depth must be sputtered prior to the depth of analyticall interest. This problem can be exacerbated on LED chips which is covered thick electrode. When the LED chip sputtering which has thick eletrode exhibit strong nonuniform sputtering properties . These problems can be avoided if sufficient sample substrate material can be removed with adequate precision to allow SIMS depth profiling from the sample back side.
Does anyone have experience or suggestions on how to prepare back side SIMS samples? Method, Recipe for polishing step and so on..
Thanks in advance
Young Woo Jeong
==============================Original Headers============================== 10, 22 -- From jyw-at-lge.com Mon Nov 20 20:54:28 2006 10, 22 -- Received: from mail1.lge.co.kr (mail1.lge.co.kr [156.147.1.151]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAL2sR55029263 10, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Nov 2006 20:54:28 -0600 10, 22 -- Received: from [156.147.51.54] (jyw-at-lge.com) by 10, 22 -- mail1.lge.co.kr (Terrace MailWatcher) 10, 22 -- with ESMTP id 2006112111:54:22:863195.12230.155 10, 22 -- for {Microscopy-at-microscopy.com} ; 10, 22 -- Tue, 21 Nov 2006 11:54:22 +0900 (KST) 10, 22 -- Subject: TEM sample preparation for back side SIMS analysis 10, 22 -- Reply-To: "=?EUC-KR?B?waS/tb/s?=" {jyw-at-lge.com} 10, 22 -- From: "=?EUC-KR?B?waS/tb/s?=" {jyw-at-lge.com} 10, 22 -- To: Microscopy-at-microscopy.com 10, 22 -- Message-ID: {OF8DE4D82F.1F7B65DD-ON4925722D.000FF6CD-4925722D.000FF6CF-at-lge.com} 10, 22 -- Date: Tue, 21 Nov 2006 11:54:22 +0900 10, 22 -- Return-Receipt-To: "=?EUC-KR?B?waS/tb/s?=" {jyw-at-lge.com} 10, 22 -- X-MIMETrack: Serialize by Router on LGEKRHQMS03/LGE/LG Group(Release 6.5.4FP2HF559 | September 10, 22 -- 7, 2006) at 2006/11/21 11:54:22 10, 22 -- MIME-Version: 1.0 10, 22 -- Content-type: text/plain; charset=US-ASCII 10, 22 -- X-TERRACE-SPAMMARK: NOT spam-marked. 10, 22 -- (by Terrace) ==============================End of - Headers==============================
EBIC and specimen current are not the same thing. For specimen current imaging, you image the balance between the incident beam current (Ib), the current due to backscatters (Ibse), the current due to secondaries (Ise) and the specimen current (Isc), such that:
Ib=Ibse+Ise+Isc
Or, Isc = Ib - Ibse - Ise
Thus, specimen current is like looking at the opposite of the backscatter and secondary signals.
EBIC, on the other hand, is a semiconductor-specific technique. In specimen current, you put the current amplifier between the sample and ground. In EBIC, however, you put the current amplifier between the p- and n- sides of an electrically active diode. When beam electrons hit the sample, they can excite it in a way similar to light falling on a solar cell. The energy imparted by the beam causes current to flow through the external amplifier. EBIC then lets you image areas of high or low electron-hole-pair recombination activity in the imaged device. If you ground the n-side and operate above the unity yield point, you'll suppress most of the specimen current contribution and therefore most or all of the topography and get a purely semiconductor-device-physics signal.
EBIC signals will often be a few orders of magnitude higher than the beam current, whereas specimen currents tend to be on the same order as the beam current.
Chad Parish
==============================Original Headers============================== 8, 24 -- From chad.parish-at-gmail.com Tue Nov 21 06:15:44 2006 8, 24 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.232]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kALCFhDc018992 8, 24 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2006 06:15:43 -0600 8, 24 -- Received: by wx-out-0506.google.com with SMTP id h30so2392523wxd 8, 24 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2006 04:15:43 -0800 (PST) 8, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 8, 24 -- s=beta; d=gmail.com; 8, 24 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 8, 24 -- b=PhGzvNQ7UC97xmlnOuDy2I4ZtWOK7ralwb1CWANlAx7R7l4kvWqZsSJ71WhBVP7mDaXtIlVgsIxqCeSsTvrw+OpmJ0ORQCN+3vIOWlb8jbBJdm9ox0CIHAi3vBmSAPsVIPuU+mnlxJ8HZhs3zez4ZQ1Y8ND+kFMVxUWuzXdYoaM= 8, 24 -- Received: by 10.90.51.17 with SMTP id y17mr5096615agy.1164111343406; 8, 24 -- Tue, 21 Nov 2006 04:15:43 -0800 (PST) 8, 24 -- Received: by 10.35.93.9 with HTTP; Tue, 21 Nov 2006 04:15:42 -0800 (PST) 8, 24 -- Message-ID: {fd59a7d40611210415u5fac01c3m87d4af1b567b2cba-at-mail.gmail.com} 8, 24 -- Date: Tue, 21 Nov 2006 07:15:42 -0500 8, 24 -- From: "Chad Parish" {chad.parish-at-gmail.com} 8, 24 -- To: microscopy-at-microscopy.com 8, 24 -- Subject: Re: [Microscopy] EBIC vs. SE 8, 24 -- In-Reply-To: {200611210240.kAL2eRDe028476-at-ns.microscopy.com} 8, 24 -- MIME-Version: 1.0 8, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 24 -- Content-Transfer-Encoding: 7bit 8, 24 -- Content-Disposition: inline 8, 24 -- References: {200611210240.kAL2eRDe028476-at-ns.microscopy.com} ==============================End of - Headers==============================
Being somewhat experienced in TEM, I am starting to work on a SEM (Tescan). The SEM is equipped with rotatory pump and TMP. There are 3 detectors: SE, BSE and EDX. In the documentation (VEGA software) it is written that the microscope can work in 2 modes: low vacuum or high vacuum. The high vacuum is allowed under a vacuum of 5x10-3 Pa. When I open the manual valve (to allow connections between the column and the chamber, thus to work in high-vacuum mode) and start pumping, the vacuum reaches no better than 8x10-3 Pa. In this condition I can only work in low vacuum mode. Nothing seems to go wrong, I wonder why I cannot reach higher vacuum?? Also, can get good results with the EDX detector in low-vacuum mode (well it is still 8x10-3 Pa)?
Stephane
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==============================Original Headers============================== 6, 18 -- From nizets2-at-yahoo.com Tue Nov 21 06:22:37 2006 6, 18 -- Received: from web37412.mail.mud.yahoo.com (web37412.mail.mud.yahoo.com [209.191.91.144]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kALCMbth025087 6, 18 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2006 06:22:37 -0600 6, 18 -- Received: (qmail 9197 invoked by uid 60001); 21 Nov 2006 12:22:36 -0000 6, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 18 -- s=s1024; d=yahoo.com; 6, 18 -- h=Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 6, 18 -- b=W23HU9Dsq7JxrlCsa5+iOP+g9giBMBtLEH6CcWhPl5Z3uxG/LrUnQbfLP1Eva5kyblg52vIL4UpngrJGhzFHohPVFkEWjSrsiS/+hkPCJkEoX0KI/QsQOvzXvAGMf2uzhpMzjbkxdOfsgcksJPEw8vrZy9+f+I2hC1DvMwpcZB4=; 6, 18 -- Received: from [80.122.101.102] by web37412.mail.mud.yahoo.com via HTTP; Tue, 21 Nov 2006 04:22:36 PST 6, 18 -- Date: Tue, 21 Nov 2006 04:22:36 -0800 (PST) 6, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 18 -- Subject: Basic SEM questions 6, 18 -- To: microscopy-at-microscopy.com 6, 18 -- MIME-Version: 1.0 6, 18 -- Content-Type: text/plain; charset=iso-8859-1 6, 18 -- Content-Transfer-Encoding: 8bit 6, 18 -- Message-ID: {609645.8917.qm-at-web37412.mail.mud.yahoo.com} ==============================End of - Headers==============================
Being somewhat experienced in TEM, I am starting to work on a SEM (Tescan). The SEM is equipped with rotatory pump and TMP. There are 3 detectors: SE, BSE and EDX. In the documentation (VEGA software) it is written that the microscope can work in 2 modes: low vacuum or high vacuum. The high vacuum is allowed under a vacuum of 5x10-3 Pa. When I open the manual valve (to allow connections between the column and the chamber, thus to work in high-vacuum mode) and start pumping, the vacuum reaches no better than 8x10-3 Pa. In this condition I can only work in low vacuum mode. Nothing seems to go wrong, I wonder why I cannot reach higher vacuum?? Also, can get good results with the EDX detector in low-vacuum mode (well it is still 8x10-3 Pa)?
Stephane
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==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Tue Nov 21 06:22:40 2006 6, 19 -- Received: from web37411.mail.mud.yahoo.com (web37411.mail.mud.yahoo.com [209.191.91.143]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kALCMcBb025125 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2006 06:22:39 -0600 6, 19 -- Received: (qmail 39750 invoked by uid 60001); 21 Nov 2006 12:22:38 -0000 6, 19 -- Message-ID: {20061121122238.39748.qmail-at-web37411.mail.mud.yahoo.com} 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 6, 19 -- b=cfOvs4tchgPpQxEOAN2L8O38oH6JekU8ads8Smu5P1rdhw1aFEV78kZAXOpG1QlKCVJk4XSAZUczFHJVIsVi1Ui5zWWWlzuZ6kQPUdclIDOCVmV3ISvdVDJAW+fIREx4VC9botY8xyVxTNwKD9IlYXwxx3aHheu5hgTxuR4kKnk=; 6, 19 -- X-YMail-OSG: zxBn1kQVM1khwB2gYlNRp4xq0_aTuBa2ux8wx52BmaHO7PCUqyb8GuspW8MypmwodXaDY3nV7wM81XghDrZCNHl96_E5kC9A15mPeBrhKnv1zQncRXZTuWa22qgiTjhH5AwB_KlBA.CoM_k- 6, 19 -- Received: from [80.122.101.102] by web37411.mail.mud.yahoo.com via HTTP; Tue, 21 Nov 2006 04:22:38 PST 6, 19 -- Date: Tue, 21 Nov 2006 04:22:38 -0800 (PST) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 -- Subject: Basic SEM questions 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
My main advice would be: be careful with quantification of fluorescence. And also arm yourself with a lot of patience. If you consider that the amount of fluorescence will vary with the focus, you will have to focus each cell for the maximum fluorescence for a given organelle (don't measure several cells in one field). Don't use automatic exposition time, otherwise you cannot compare your results. Choose carefully one and only one exposition time and deal with it for ALL your cells. To avoid bias due to bleaching, you should determine a time period during which you make your adjustments (focus and field) before taking the picture and use the same time for ALL your cells (I think that photobleaching is an important factor with GPF constructs). Be careful not to choose a cell which was already exposed (in the field of view of another cell of interest). Now if you have an option to automatize the task, I guess these biases would be averaged by measuring several hundreds of cells (though bleaching will still be an issue, but you can control it).
Good luck Stephane
--- moakes-at-HCRcenter.com wrote:
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==============================Original Headers============================== 12, 20 -- From nizets2-at-yahoo.com Tue Nov 21 06:26:52 2006 12, 20 -- Received: from web37411.mail.mud.yahoo.com (web37411.mail.mud.yahoo.com [209.191.91.143]) 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kALCQq5p006396 12, 20 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2006 06:26:52 -0600 12, 20 -- Received: (qmail 40682 invoked by uid 60001); 21 Nov 2006 12:26:51 -0000 12, 20 -- Message-ID: {20061121122651.40680.qmail-at-web37411.mail.mud.yahoo.com} 12, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 20 -- s=s1024; d=yahoo.com; 12, 20 -- h=Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 12, 20 -- b=jLmF+79C7Rtea/6OjxjoumspTTzcIZ8DrQDhnQJomgduKgiC3NSFO1Ev7l28gt7kLgUn7cAky0fVTFPaYXY5QtNk6kopiqGOVknAz0Aga27S+SK9iBG6EjrHBV2/MWRmLQbs87wgoPZyhmrUW1y36ywhj2xEDVTV1hKkmuK/XcE=; 12, 20 -- Received: from [80.122.101.102] by web37411.mail.mud.yahoo.com via HTTP; Tue, 21 Nov 2006 04:26:51 PST 12, 20 -- Date: Tue, 21 Nov 2006 04:26:51 -0800 (PST) 12, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 12, 20 -- Subject: Re: [Microscopy] viaWWW: measurement of fluorescence 12, 20 -- To: moakes-at-HCRcenter.com 12, 20 -- Cc: microscopy-at-microscopy.com 12, 20 -- In-Reply-To: {200611181439.kAIEdBUC027262-at-ns.microscopy.com} 12, 20 -- MIME-Version: 1.0 12, 20 -- Content-Type: text/plain; charset=iso-8859-1 12, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I do not known the procedures on your microscope but others with a similar format would show this type of problem under the following conditions.
1. A genuine leak in the specimen chamber 2. The control valve for the VP (low vacuum) operation is not fully closed. 3. The valve between the VP pumping system and the chamber is not fully closed thus you are pumping against the second rotary pump.
Hope this may help?
Steve Chapman Senior Consultant Protrain For electron microscopy consultancy and training world wide Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967 Web www.emcourses.com
----- Original Message ----- X-from: {nizets2-at-yahoo.com} To: {protrain-at-emcourses.com} Sent: Tuesday, November 21, 2006 12:23 PM
X-from: "Dr. Michael Horst" {HORST_MN-at-Mercer.edu} To: "Greg Erdos" {gwe-at-ufl.edu} Sent: Tuesday, November 21, 2006 9:00 AM
Thanks to all who responded to my query about ammonium molybdate not wanting to dissolve. A couple people had questions about why I was using 5% AM, rather than 1-2%. The reason is simply that I was going by a recent Microscopy and Analysis May, 2006 article on developments in neg staining as my starting point. I may try a lesser concentration. It's experiment time.
Everything finally went into solution, by the way, especially after I adjusted the pH to 7.4 with NaOH.
I also tried 0.5% UA with 12mM oxalic acid, which supposedly allows pH adjustment into the physiological range. All was well until I got near ph 7.3, when it looked like all the UA precipitated out, leaving a nearly clear solution behind. I'm gonna try it anyway. You just never know.
Thanks again. If anyone's interested, I'll keep them posted on my negative (staining) results.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 7, 23 -- From TindallR-at-missouri.edu Tue Nov 21 08:50:37 2006 7, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kALEobiI020778 7, 23 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2006 08:50:37 -0600 7, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 7, 23 -- Tue, 21 Nov 2006 08:50:36 -0600 7, 23 -- x-mimeole: Produced By Microsoft Exchange V6.5 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="us-ascii" 7, 23 -- Subject: Negative staining: Thanks 7, 23 -- Date: Tue, 21 Nov 2006 08:50:36 -0600 7, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68BDC-at-UM-XMAIL08.um.umsystem.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- Thread-Topic: Negative staining: Thanks 7, 23 -- Thread-Index: AccNfGd/rQsnGPh5R9Kmljbz31d4dA== 7, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 7, 23 -- To: {microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 21 Nov 2006 14:50:36.0947 (UTC) FILETIME=[67A74630:01C70D7C] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kALEobiI020778 ==============================End of - Headers==============================
Hi Jackie I regularly work with formvar/carbon coated slot grids with over 100 serial sections on them. I feel your pain. I have done two things; 1) be very careful - you can use grid sticks or tweezers, but you have to be very careful 2) change your embedding techniques so that you do not have to stain on the grid.
I know that #1 is not very useful :-) I have developed an embedding technique that does not require staining on the grid. Both UA and Pb are done in-block. The 50nm sections have fine contrast. This means that I do not have to stain the grids and risk the sections or the formvar. If you want the protocol, let me know. David
_____________________
David Elliott Ph.D. Assistant Professor - Department of Cell Biology and Anatomy Director, Research Microscopy Core Service University of Arizona College of Medicine PO Box 245004 Tucson, AZ 85724
Voice: 520-626-7870 Fax: 520-626-2097
On Nov 20, 2006, at 5:17 PM, jcraft-at-memphis.edu wrote:
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==============================Original Headers============================== 12, 22 -- From Elliott-at-arizona.edu Tue Nov 21 09:39:23 2006 12, 22 -- Received: from smtpgate.email.arizona.edu (deagol.email.Arizona.EDU [128.196.133.142]) 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kALFdMVu032200 12, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Nov 2006 09:39:22 -0600 12, 22 -- Received: from localhost (amavis5.email.arizona.edu [10.0.0.208]) 12, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP 12, 22 -- id 7F296121CC52; Tue, 21 Nov 2006 08:39:22 -0700 (MST) 12, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 12, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP 12, 22 -- id 00767121C1FD; Tue, 21 Nov 2006 08:39:20 -0700 (MST) 12, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 12, 22 -- In-Reply-To: {200611210017.kAL0HKv5004869-at-ns.microscopy.com} 12, 22 -- References: {200611210017.kAL0HKv5004869-at-ns.microscopy.com} 12, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 12, 22 -- Message-Id: {0AAF81E5-6A33-4B38-8964-6739CAA635D9-at-arizona.edu} 12, 22 -- Content-Transfer-Encoding: 7bit 12, 22 -- From: David Elliott {Elliott-at-arizona.edu} 12, 22 -- Subject: Re: [Microscopy] AskAMicroscopist: staining multiple formvar/carbon coated slot 12, 22 -- Date: Tue, 21 Nov 2006 08:39:18 -0700 12, 22 -- To: jcraft-at-memphis.edu, Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 12, 22 -- X-Mailer: Apple Mail (2.752.2) 12, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
I still think in terms of Torr, so I had to convert your numbers over.
You say "high vacuum is allowed under a vacuum of 5x10-3 Pa". I convert that to about 4x10-5 Torr. You also say that you only get to 8x10-3 Pa when you open the manual valve between the column and the chamber. I convert that to 6x10-5 Torr.
That does sound like a poor vacuum. Our old JEOL 840A drops below 1x10-6 Torr on a good day with a diffusion pump. I think the safety interlock prevents operation above pressures of ~4x10-5 Torr. In that regard, the two microscopes seem consistent.
I agree with Chapman that this might indicate a leak in your chamber or perhaps you are not setting up the pumping properly. I have no idea what the proper procedure is for a Tescan. Our Hitachi S-2460N automates the process.
Regarding EDS, you should be able to use it quite well at 8x10-3 Pa. You will increasingly scatter the primary beam as the pressure increases. However, we do a lot of EDS work at 40 Pa (0.3 Torr). We simply have to take scattering into consideration. I greatly prefer "high" vacuum for quantitative EDS. It avoids complicating the spectrum of my analysis point with signal from the neighborhood.
Warren
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, November 21, 2006 6:24 AM To: wesaia-at-iastate.edu
I agree that EBIC is not SC. In my case, it is a matter of conduction rather than true EBIC. I'm not looking at junctions, etc. The idea is to measure and image using specimen current and compliment with SE and or BSE. I image with SC and mix with SE or BSE and get quite interesting results.
In some cases, I'm using non-conductive specimens. In these cases, the beam current conductive portions have higher SC than areas that are non-conductive. However, in working with STEM specimens of IC cross sections, the metal conducts a certain amount while the passivation and ILD allows the beam to pass through. Depending on many factors, the SC can be radically different.
I suppose I'm just thinking at the keyboard. But I do wonder what the physics is behind all of this practical application.
gary g.
At 04:18 AM 11/21/2006, you wrote:
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==============================Original Headers============================== 13, 20 -- From gary-at-gaugler.com Tue Nov 21 16:58:13 2006 13, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kALMwCaf002355 13, 20 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2006 16:58:13 -0600 13, 20 -- Received: (qmail 11399 invoked from network); 21 Nov 2006 14:58:06 -0800 13, 20 -- Received: by simscan 1.1.0 ppid: 11363, pid: 11396, t: 0.1894s 13, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 13, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 13, 20 -- by qsmtp1 with SMTP; 21 Nov 2006 14:58:06 -0800 13, 20 -- Message-Id: {7.0.1.0.2.20061121145655.025eaae0-at-gaugler.com} 13, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 13, 20 -- Date: Tue, 21 Nov 2006 14:58:05 -0800 13, 20 -- To: chad.parish-at-gmail.com 13, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 13, 20 -- Subject: Re: [Microscopy] Re: EBIC vs. SE 13, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 13, 20 -- In-Reply-To: {200611211218.kALCIn7D021934-at-ns.microscopy.com} 13, 20 -- References: {200611211218.kALCIn7D021934-at-ns.microscopy.com} 13, 20 -- Mime-Version: 1.0 13, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
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Email: eoptics-at-mcmaster.ca Name: Fred Pearson
Organization: McMaster University
Title-Subject: [Filtered] Require Manual for HB601
Question: We have acquired a Vacuum Generators HB601 and would like to obtain instructions for alignment and other operating conditions. The manual that arrived with the instrument is good but someone out there might have written a more detailed alignment procedure. You can contact me offline if you wish.
This Question was submitted to Ask-A-Microscopist by (patrick.mccurdy-at-colostate.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, November 21, 2006 at 10:51:23 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both patrick.mccurdy-at-colostate.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: patrick.mccurdy-at-colostate.edu Name: Pat McCurdy
Organization: Colorado State University
Education: Graduate College
Location: Fort Collins, CO, USA
Title: FE filiment lifetime
Question: According to Goldstein the life of a field emission Schotky filament is ~1 year. JEOL's service manual also states the lifetime of the emitter is ~1 year. The reason stated by both sources is the limited reservoir of zirconia surrounding the tip, which lowers the work function. Our emitter is now almost 5 years old. Why the discrepancy?
That is an interesting question. What I see is about two years of life. However, the variables are brand of FE tip and gun chamber vacuum and duty cycle of gun on/off. My gun chamber vacuum is about 1.5E-10 Torr.
For a Denka tip on all the time, I am seeing at least two years. The following SEM pix shows a Denka tip at two years and there is plenty of ZrO2. There have been FEI tips in my SEM but none worked. The way Zeiss does this is to cull out the losers (Denka and FEI) and only deliver the ones that pass their scrutiny. It does make a difference. I'm not sure what criteria they use but it works.
http://www.gaugler.com/fe%20tip-2.jpg
I was prepared for a tip replacement every year but do not experience this need at all. The gun is on 24/7. It is very stable and unflinching in Iext. This is in a Zeiss Supra 55VP.
Disclaimer: No financial interest in Zeiss, Denka or FEI other than to keep Zeiss supporting my Supra 55VP.
gary g.
At 05:40 PM 11/21/2006, you wrote:
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==============================Original Headers============================== 14, 20 -- From gary-at-gaugler.com Tue Nov 21 20:59:28 2006 14, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kAM2xSjS006453 14, 20 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2006 20:59:28 -0600 14, 20 -- Received: (qmail 31868 invoked from network); 21 Nov 2006 18:59:27 -0800 14, 20 -- Received: by simscan 1.1.0 ppid: 31817, pid: 31866, t: 0.1798s 14, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 14, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 14, 20 -- by qsmtp3 with SMTP; 21 Nov 2006 18:59:27 -0800 14, 20 -- Message-Id: {7.0.1.0.2.20061121184818.025be488-at-gaugler.com} 14, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 14, 20 -- Date: Tue, 21 Nov 2006 18:59:21 -0800 14, 20 -- To: patrick.mccurdy-at-colostate.edu 14, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 14, 20 -- Subject: Re: [Microscopy] AskAMicroscopist: FE filiment lifetime 14, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 14, 20 -- In-Reply-To: {200611220140.kAM1eiwf021916-at-ns.microscopy.com} 14, 20 -- References: {200611220140.kAM1eiwf021916-at-ns.microscopy.com} 14, 20 -- Mime-Version: 1.0 14, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
We have just installed our first Schottky FE (Hitachi 4300 SE/N.) There is plenty of current, and therefore a bit of temptation to wind the filament temperature back just a little to - maybe? - increase lifetime and resolution. Has any one tried this, does anyone know how the optimal temperature is determined? ZrO2 mobility, gas absorption, electron emission, energy spread? ...are there other factors?
Sally
Dr SJ Stowe Facility Coordinator ANU Electron Microscopy Unit ANU CRICOS#00120C
} -----Original Message----- } From: gary-at-gaugler.com [mailto:gary-at-gaugler.com] } Sent: Wednesday, 22 November 2006 2:00 PM } To: sally.stowe-at-anu.edu.au } Subject: [Microscopy] Re: AskAMicroscopist: FE filiment lifetime } } } } } } --------------------------------------------------------------- } ------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
==============================Original Headers============================== 10, 26 -- From sally.stowe-at-anu.edu.au Tue Nov 21 23:28:27 2006 10, 26 -- Received: from mail.rsbs.anu.edu.au (mail.rsbs.anu.edu.au [150.203.72.70]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAM5SQXO020020 10, 26 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2006 23:28:26 -0600 10, 26 -- Received: from rsbs2262 (rsbs2262.rsbs.anu.edu.au [150.203.36.144]) 10, 26 -- by mail.rsbs.anu.edu.au (Postfix) with ESMTP 10, 26 -- id C6360F44E6A; Wed, 22 Nov 2006 16:28:14 +1100 (EST) 10, 26 -- Reply-To: {sally.stowe-at-anu.edu.au} 10, 26 -- From: "Sally Stowe" {sally.stowe-at-anu.edu.au} 10, 26 -- To: {gary-at-gaugler.com} , {microscopy-at-microscopy.com} 10, 26 -- Subject: RE: [Microscopy] Re: AskAMicroscopist: FE filiment lifetime 10, 26 -- Date: Wed, 22 Nov 2006 16:28:14 +1100 10, 26 -- Message-ID: {004b01c70df7$022e4b20$9024cb96-at-rsbs.anu.edu.au} 10, 26 -- MIME-Version: 1.0 10, 26 -- Content-Type: text/plain; 10, 26 -- charset="us-ascii" 10, 26 -- Content-Transfer-Encoding: 7bit 10, 26 -- X-Priority: 3 (Normal) 10, 26 -- X-MSMail-Priority: Normal 10, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 10, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1506 10, 26 -- Importance: Normal 10, 26 -- In-Reply-To: {200611220259.kAM2xdeX006634-at-ns.microscopy.com} 10, 26 -- X-RSBS-MailScanner-Information: Please contact the ISP for more information 10, 26 -- X-RSBS-MailScanner: Found to be clean 10, 26 -- X-MailScanner-From: sally.stowe-at-anu.edu.au ==============================End of - Headers==============================
I wanted to thank you all for the numerous answers I got for my problem. Well I still had to go through the whole alignment procedure(what I wanted to avoid by mailing this list - I thought it was possible through the direct alignments) but the problem is fixed. Many thanks to the FEI people for their care.
Stephane
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==============================Original Headers============================== 6, 20 -- From nizets2-at-yahoo.com Wed Nov 22 06:41:50 2006 6, 20 -- Received: from web37401.mail.mud.yahoo.com (web37401.mail.mud.yahoo.com [209.191.91.133]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kAMCfnwk007431 6, 20 -- for {microscopy-at-microscopy.com} ; Wed, 22 Nov 2006 06:41:49 -0600 6, 20 -- Received: (qmail 70474 invoked by uid 60001); 22 Nov 2006 12:41:47 -0000 6, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 20 -- s=s1024; d=yahoo.com; 6, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 6, 20 -- b=C4oCxW3ghfm43r471SAVrnUKUd2vWPL0fpMc8aiYp7dST7jYaUHv1x31rSNBaezgpEMFNElJvKcUOHVhS73VfK9EoClf7F4uL4k1aSrnBnxFEPR6spIcj8v00mf6bq9yppzdKHy/ghwrrDEEBPoc/HbgYZs86v9eKcFBU1xghnM=; 6, 20 -- X-YMail-OSG: RbeIZuMVM1kf5RSAkb2d2u3j_lPRaQXsDTuIf2AD 6, 20 -- Received: from [80.122.101.102] by web37401.mail.mud.yahoo.com via HTTP; Wed, 22 Nov 2006 04:41:47 PST 6, 20 -- Date: Wed, 22 Nov 2006 04:41:47 -0800 (PST) 6, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 20 -- Subject: RE: [Microscopy] alignment with tecnai twin G20 - Thanks 6, 20 -- To: microscopy-at-microscopy.com 6, 20 -- In-Reply-To: {00948E5E66F1374FB9284A7B78138EB806E6CAD7-at-hlexc03.w2k.feico.com} 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; charset=iso-8859-1 6, 20 -- Content-Transfer-Encoding: 8bit 6, 20 -- Message-ID: {481904.70280.qm-at-web37401.mail.mud.yahoo.com} ==============================End of - Headers==============================
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Title-Subject: [Filtered] Insulin and glucagon staining in the pancreas
Question: Hi All. I was wanting to do some immunohistochemistry on paraffin pancreas sections. I would like to double stain the sections with insulin and glucagon, then count the number of islets relative to the whole area of the pancreas. Has anyone does such staining before using DAB? Thanks in advance, Emily
Sam, My experience is that Larry is absolutely correct. You'll be starting at room temperature and the temperature is likely to rise considerably and rapidly at the beam. On the other hand, holding a specimen below 4 C wouldn't be very difficult using a thermoelectric (Peltier) device. All that is needed is an electrical feedthrough (2 connections for the device and an optional 2 connections for a thermocouple to read temperature and/or provide feedback to an automatic controller) and a heatsink connection from the hot side of the device to the wall of the stage or chamber (several pieces of ground braid can provide a flexible thermal connection). This is assuming that you want to be able to vary the temperature at will.
On the other hand, if all you want to do is immobilize your sample either for a quick look, or to watch what happens as the sample becomes mobilized, than I would think that chilling the Quantomix capsule by some amount would take care of that. How much would most likely have to be determined by experiment.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: larry-at-celtic.freewire.co.uk [mailto:larry-at-celtic.freewire.co.uk] Sent: Tuesday, November 14, 2006 4:08 PM To: kenconverse-at-qualityimages.biz
} ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America I guess you actually mean the temperature under the beam ..... The temperature in the specimen chamber generally is going to be close to ambient (except for an ESEM, were the sample will be ~5 deg C)
Key things to consider are probe current, scan speed, magnification and thermal conduction path away from the imaged area. In the case of Quantomix capsules, with a FEG-SEM it is relatively easy to punch holes in the membrane with the beam, rather less so with W SEMs.
It is also relatively easy in most SEMs to find conditions which will melt lower temperature thermosetting polymers, so local sample temperatures in the 50 to 100 deg C aren't too difficult to generate, with the right sample.
Basically, I think there are so many variables involved, you're just going to have to try it.
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==============================Original Headers============================== 6, 16 -- From larry-at-celtic.freewire.co.uk Tue Nov 14 15:04:26 2006 6, 16 -- Received: from get.freewire.net (get.freewire.net [86.54.106.202] (may be forged)) 6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAEL4Nue017767 6, 16 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 14 Nov 2006 15:04:26 -0600 6, 16 -- Received: from [194.164.78.253] (modem-rack4-modem253.netkonect.net [194.164.78.253]) 6, 16 -- by get.freewire.net (8.13.6/8.11.6) with ESMTP id kAEL2b3B004597; 6, 16 -- Tue, 14 Nov 2006 21:03:31 GMT 6, 16 -- Mime-Version: 1.0 6, 16 -- Message-Id: {p06210202c17fd7b6ceb4-at-[194.164.78.26]} 6, 16 -- In-Reply-To: {200611141429.kAEETu5l019725-at-ns.microscopy.com} 6, 16 -- References: {200611141429.kAEETu5l019725-at-ns.microscopy.com} 6, 16 -- Date: Tue, 14 Nov 2006 20:38:18 +0000 6, 16 -- To: Sam.Murray-at-port.ac.uk, Microscopy-at-MSA.Microscopy.Com 6, 16 -- X-from: Larry Stoter {larry-at-celtic.freewire.co.uk} 6, 16 -- Subject: Re: [Microscopy] Temperature inside SEM chamber? 6, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 22, 28 -- From kenconverse-at-qualityimages.biz Wed Nov 22 09:16:09 2006 22, 28 -- Received: from dpmailmta02.doteasy.com (dpmailmta02.doteasy.com [65.61.209.84]) 22, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAMFG8BX032011 22, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 22 Nov 2006 09:16:09 -0600 22, 28 -- Received: from qualityimages.biz (unverified [192.168.101.16]) 22, 28 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id 22945706-1814644 22, 28 -- for multiple; Wed, 22 Nov 2006 07:29:24 -0800 22, 28 -- Received: from Ken [76.179.237.214] by qualityimages.biz with ESMTP 22, 28 -- (SMTPD32-8.05) id A99A2470218; Wed, 22 Nov 2006 07:15:38 -0800 22, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 22, 28 -- To: {larry-at-celtic.freewire.co.uk} , 22, 28 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 22, 28 -- Subject: RE: [Microscopy] Re: Temperature inside SEM chamber? 22, 28 -- Date: Wed, 22 Nov 2006 10:15:02 -0500 22, 28 -- Message-ID: {007701c70e49$08583b30$6401a8c0-at-Ken} 22, 28 -- MIME-Version: 1.0 22, 28 -- Content-Type: text/plain; 22, 28 -- charset="us-ascii" 22, 28 -- X-Priority: 3 (Normal) 22, 28 -- X-MSMail-Priority: Normal 22, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 22, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 22, 28 -- Importance: Normal 22, 28 -- In-Reply-To: {200611142108.kAEL85ZC022598-at-ns.microscopy.com} 22, 28 -- X-IP-stats: Incoming Last 0, First 55, in=1250933, out=0, spam=0 ip=192.168.101.16 22, 28 -- X-External-IP: 192.168.101.16 22, 28 -- Content-Transfer-Encoding: 8bit 22, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAMFG8BX032011 ==============================End of - Headers==============================
Our JEOL FETEM is on the third emitter in over nine years. We replaced one when moving the microscope (-at- three years), and another failed three years later. We believe JEOL has just been very conservative in rating the tip life. Five years, though, is extraordinary.
John Mardinly Intel
This is the opinion of the author and does not represent the position of Intel Corporation.
-----Original Message----- X-from: patrick.mccurdy-at-colostate.edu [mailto:patrick.mccurdy-at-colostate.edu] Sent: Tuesday, November 21, 2006 5:39 PM To: Mardinly, John
This Question was submitted to Ask-A-Microscopist by (patrick.mccurdy-at-colostate.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, November 21, 2006 at 10:51:23 Remember to consider the Grade/Age of the student when considering the Question ------------------------------------------------------------------------ --- Please reply to both patrick.mccurdy-at-colostate.edu as well as to the Microscopy Listserver ------------------------------------------------------------------------ ---
Email: patrick.mccurdy-at-colostate.edu Name: Pat McCurdy
Organization: Colorado State University
Education: Graduate College
Location: Fort Collins, CO, USA
Title: FE filiment lifetime
Question: According to Goldstein the life of a field emission Schotky filament is ~1 year. JEOL's service manual also states the lifetime of the emitter is ~1 year. The reason stated by both sources is the limited reservoir of zirconia surrounding the tip, which lowers the work function. Our emitter is now almost 5 years old. Why the discrepancy?
The emitter on our JEOL FETEM is now nearly 9 years old having been installed in January 1998 (71,763hrs). We operate at a lower emission than JEOL usually use (~110microA) in order to get reasonable EELS resolution and enough beam current for Z contrast STEM.
Regards
Alan
At 10:26 AM 11/22/2006 -0600, john.mardinly-at-intel.com wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Alan W Nicholls, PhD Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 100 Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
} -----Original Message----- } From: gary-at-gaugler.com [mailto:gary-at-gaugler.com] } Sent: Tuesday, November 21, 2006 9:00 PM } To: Dusevich, Vladimir } Subject: [Microscopy] Re: AskAMicroscopist: FE filiment lifetime } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } That is an interesting question. What I see is about two years of } life. However, the variables are brand of FE tip and gun chamber } vacuum and duty cycle of gun on/off. My gun chamber vacuum is about } 1.5E-10 Torr. } } For a Denka tip on all the time, I am seeing at least two years. The } following SEM pix shows a Denka tip at two years and there is plenty } of ZrO2. There have been FEI tips in my SEM but none worked. The way
} Zeiss does this is to cull out the losers (Denka and FEI) and only } deliver the ones that pass their scrutiny. It does make a difference. } I'm not sure what criteria they use but it works. } } http://www.gaugler.com/fe%20tip-2.jpg } } I was prepared for a tip replacement every year but do not experience } this need at all. The gun is on 24/7. It is very stable and } unflinching in Iext. This is in a Zeiss Supra 55VP. } } Disclaimer: No financial interest in Zeiss, Denka or FEI other than } to keep Zeiss supporting my Supra 55VP. } } gary g. } } }
==============================Original Headers============================== 8, 23 -- From DusevichV-at-umkc.edu Wed Nov 22 13:46:06 2006 8, 23 -- Received: from kc-msxproto1.kc.umkc.edu (kc-msxproto1.kc.umkc.edu [134.193.143.167]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAMJk6JG007769 8, 23 -- for {microscopy-at-msa.microscopy.com} ; Wed, 22 Nov 2006 13:46:06 -0600 8, 23 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.1830); 8, 23 -- Wed, 22 Nov 2006 13:46:06 -0600 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="us-ascii" 8, 23 -- Subject: FW: [Microscopy] Re: AskAMicroscopist: FE filiment lifetime 8, 23 -- Date: Wed, 22 Nov 2006 13:46:06 -0600 8, 23 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB33ADC5E-at-KC-MSX1.kc.umkc.edu} 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- Thread-Topic: [Microscopy] Re: AskAMicroscopist: FE filiment lifetime 8, 23 -- Thread-Index: AccN4lUdrO923mWuQCCZw9FJyaaOhgAZQarwAAnVnTA= 8, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 8, 23 -- To: {microscopy-at-msa.microscopy.com} 8, 23 -- X-OriginalArrivalTime: 22 Nov 2006 19:46:06.0102 (UTC) FILETIME=[D9776360:01C70E6E] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAMJk6JG007769 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bairdgb-at-uwec.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bairdgb-at-uwec.edu Name: Graham Baird
Organization: University of Wisconsin-Eau Claire
Title-Subject: [Filtered] Backscatter detector for Hatachi S510 SEM
Question: Does anyone know how much a backscatter detector for a Hatachi S510 SEM could cost? Or could even send me a quote? Thanks a lot. Cheers, Graham Baird UWEC Geology Dept.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wa5ekh-at-juno.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wa5ekh-at-juno.com Name: Charles Jeffrey Day
Organization: none
Title-Subject: [Filtered] Electroscan E3 Environmental SEM and Tracor Be-Window Xray Detector
Question: Last reminder: The company I work for has descided to buy a new SEM, so they will soon sell all or part of this ESEM and Xray system (1 board in each needs repair).You can contact me by direct email.(I have no financial interest in this instrument, but I know how hard it is to find parts for these, so this may have some of the parts you need..)
EBS is the US rep for KE Developments. SPI is the US rep for Robinson. If I recall correctly, a Robinson Model 8 is about $10K, depending on options.
gary g.
At 03:50 PM 11/22/2006, you wrote:
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==============================Original Headers============================== 8, 21 -- From gary-at-gaugler.com Wed Nov 22 18:14:53 2006 8, 21 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kAN0Erps024924 8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 22 Nov 2006 18:14:53 -0600 8, 21 -- Received: (qmail 29419 invoked from network); 22 Nov 2006 16:14:52 -0800 8, 21 -- Received: by simscan 1.1.0 ppid: 29398, pid: 29417, t: 0.1700s 8, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 8, 21 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 8, 21 -- by qsmtp3 with SMTP; 22 Nov 2006 16:14:52 -0800 8, 21 -- Message-Id: {7.0.1.0.2.20061122161252.025d4390-at-gaugler.com} 8, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 8, 21 -- Date: Wed, 22 Nov 2006 16:14:47 -0800 8, 21 -- To: bairdgb-at-uwec.edu 8, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 8, 21 -- Subject: Re: [Microscopy] viaWWW: Backscatter detector for Hatachi S510 8, 21 -- SEM 8, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 8, 21 -- In-Reply-To: {200611222350.kAMNojfg008234-at-ns.microscopy.com} 8, 21 -- References: {200611222350.kAMNojfg008234-at-ns.microscopy.com} 8, 21 -- Mime-Version: 1.0 8, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Roberto Garcia, Fred Stevie, Phil Russell and there may be more authors wrote a how-to-do this paper. They are in the Materials Science Dept at North Carolina State University. Unfortunately, I can't lay my hands on the paper or I would give you the proper citation. Basically, they use a puck that is compatible with the SIMS instrument and controllably grind the edges of a sample at the same angles on four corners. They measure the thickness of layers revealed at the corners to make their tilt corrections to find the polishing surface parallel to the backside and then grind away. When they get close, they use interference fringes to make their final tilt corrections. They use a competitor's product, but I believe that the same action could easily be accomplished in the same fashion with a Tripod PolisherR and at a significantly lower cost.
Disclaimer: South Bay Technology, Inc. makes and sells the Tripod PolisherR
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: jyw-at-lge.com [mailto:jyw-at-lge.com] Sent: Monday, November 20, 2006 6:58 PM To: Walck-at-SouthBayTech.com
Hello
I need to prepare the LED chip for back side SIMS anaysis. SIMS depth profile resolution is significantly degraded if a large depth must be sputtered prior to the depth of analyticall interest. This problem can be exacerbated on LED chips which is covered thick electrode. When the LED chip sputtering which has thick eletrode exhibit strong nonuniform sputtering properties . These problems can be avoided if sufficient sample substrate material can be removed with adequate precision to allow SIMS depth profiling from the sample back side.
Does anyone have experience or suggestions on how to prepare back side SIMS samples? Method, Recipe for polishing step and so on..
Thanks in advance
Young Woo Jeong
==============================Original Headers============================== 10, 22 -- From jyw-at-lge.com Mon Nov 20 20:54:28 2006 10, 22 -- Received: from mail1.lge.co.kr (mail1.lge.co.kr [156.147.1.151]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAL2sR55029263 10, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Nov 2006 20:54:28 -0600 10, 22 -- Received: from [156.147.51.54] (jyw-at-lge.com) by 10, 22 -- mail1.lge.co.kr (Terrace MailWatcher) 10, 22 -- with ESMTP id 2006112111:54:22:863195.12230.155 10, 22 -- for {Microscopy-at-microscopy.com} ; 10, 22 -- Tue, 21 Nov 2006 11:54:22 +0900 (KST) 10, 22 -- Subject: TEM sample preparation for back side SIMS analysis 10, 22 -- Reply-To: "=?EUC-KR?B?waS/tb/s?=" {jyw-at-lge.com} 10, 22 -- From: "=?EUC-KR?B?waS/tb/s?=" {jyw-at-lge.com} 10, 22 -- To: Microscopy-at-microscopy.com 10, 22 -- Message-ID: {OF8DE4D82F.1F7B65DD-ON4925722D.000FF6CD-4925722D.000FF6CF-at-lge.com} 10, 22 -- Date: Tue, 21 Nov 2006 11:54:22 +0900 10, 22 -- Return-Receipt-To: "=?EUC-KR?B?waS/tb/s?=" {jyw-at-lge.com} 10, 22 -- X-MIMETrack: Serialize by Router on LGEKRHQMS03/LGE/LG Group(Release 6.5.4FP2HF559 | September 10, 22 -- 7, 2006) at 2006/11/21 11:54:22 10, 22 -- MIME-Version: 1.0 10, 22 -- Content-type: text/plain; charset=US-ASCII 10, 22 -- X-TERRACE-SPAMMARK: NOT spam-marked. 10, 22 -- (by Terrace) ==============================End of - Headers==============================
==============================Original Headers============================== 19, 22 -- From walck-at-southbaytech.com Wed Nov 22 20:23:20 2006 19, 22 -- Received: from ylpvm12.prodigy.net (ylpvm12-ext.prodigy.net [207.115.57.43]) 19, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAN2NK5L011211 19, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Nov 2006 20:23:20 -0600 19, 22 -- X-ORBL: [64.169.193.90] 19, 22 -- Received: from dynamicbl8uno3 (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90]) 19, 22 -- by ylpvm12.prodigy.net (8.13.7 out spool5000 dk/8.13.7) with ESMTP id kAN2MYYM027762; 19, 22 -- Wed, 22 Nov 2006 21:22:35 -0500 19, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} 19, 22 -- To: {Microscopy-at-microscopy.com} 19, 22 -- Cc: {jyw-at-lge.com} 19, 22 -- Subject: RE: [Microscopy] TEM sample preparation for back side SIMS analysis 19, 22 -- Date: Wed, 22 Nov 2006 18:23:41 -0800 19, 22 -- Message-ID: {00aa01c70ea6$64e75270$7801a8c0-at-dynamicbl8uno3} 19, 22 -- MIME-Version: 1.0 19, 22 -- Content-Type: text/plain; 19, 22 -- charset="us-ascii" 19, 22 -- Content-Transfer-Encoding: 7bit 19, 22 -- X-Mailer: Microsoft Office Outlook 11 19, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 19, 22 -- Thread-Index: AccNGM/MlHmt7yWYRGSN6cTiH8iVoAAelYBA 19, 22 -- In-Reply-To: {200611210257.kAL2vfXA002764-at-ns.microscopy.com} ==============================End of - Headers==============================
Graham Baird wrote: =================================================== Does anyone know how much a backscatter detector for a Hatachi S510 SEM could cost? Or could even send me a quote? Thanks a lot. ================================================== Full information about the Robinson backscattered electron detector can be found on URL http://2spi.com/catalog/instruments/robinson-backscattered-electron-BSE-detector.shtml
There is an on-line questionnaire that can be filled out and once we have the details of your installation and requirements, we can give you a recommendation and firm price quote.
Chuck
PS: Remember that we are striving to be 100% paperless, therefore there are no paper copies kept of this correspondence. Please be sure to always reply by way of "reply" on your software so that the entire string of correspondence can be kept in one place.
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
==============================Original Headers============================== 10, 26 -- From cgarber-at-2spi.com Wed Nov 22 22:45:10 2006 10, 26 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAN4jArA027629 10, 26 -- for {microscopy-at-msa.microscopy.com} ; Wed, 22 Nov 2006 22:45:10 -0600 10, 26 -- Received: from ibm1x23g2abfyg (000-191-484.area3.spcsdns.net [68.246.233.20] (may be forged)) 10, 26 -- (authenticated bits=0) 10, 26 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id kAN4j2s9021132 10, 26 -- for {microscopy-at-msa.microscopy.com} ; Wed, 22 Nov 2006 23:45:09 -0500 10, 26 -- X-IDV-FirstRcvd: 000-191-484.area3.spcsdns.net [68.246.233.20] (may be forged) 10, 26 -- X-IDV-HELO: ibm1x23g2abfyg 10, 26 -- X-IDV-Authenticated-User: cgarber 10, 26 -- Message-ID: {00b501c70eba$244ab450$14e9f644-at-ibm1x23g2abfyg} 10, 26 -- Reply-To: "Garber, Charles A." {cgarber-at-2spi.com} 10, 26 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 10, 26 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 10, 26 -- Subject: Backscattered electron detectors 10, 26 -- Date: Wed, 22 Nov 2006 23:44:20 -0500 10, 26 -- MIME-Version: 1.0 10, 26 -- Content-Type: text/plain; 10, 26 -- charset="Windows-1252" 10, 26 -- X-Priority: 3 10, 26 -- X-MSMail-Priority: Normal 10, 26 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2869 10, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 10, 26 -- Content-Transfer-Encoding: 8bit 10, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAN4jArA027629 ==============================End of - Headers==============================
I finally found the necessary sequence to have "green light" for "high vacuum" mode. Funnily, the vacuum remains the same, but now I have a green signal and I can activate the HT and filament! Here are some few more questions:
1. In TEM one needs to tilt the sample to improve the signal by EDX. Is it the same in SEM? (considering that the detector is also at an angle above the sample) 2. Is it realistic to use HT of 5kV or 10kV to take EDX spectra by SEM? 3. Resolution issue: while observing mineral particules (quarz for example), when I reach a magnification of 2kx or higher (to observe particles several µm big for example), the image is not sharp. Using the mode which offers the maximum resolution (cleverly called “resolution mode”), the beam reaches a size of 60nm. In these conditions it is not surprising that the image is not sharp. The SEM allows magnification of up to 500kx!! What is the point to use so high magnification if the image is blurred? What is the expected resolution of a SEM?
Stephane
____________________________________________________________________________________ Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail beta. http://new.mail.yahoo.com
==============================Original Headers============================== 7, 19 -- From nizets2-at-yahoo.com Thu Nov 23 09:39:03 2006 7, 19 -- Received: from web37415.mail.mud.yahoo.com (web37415.mail.mud.yahoo.com [209.191.91.147]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kANFd2jH020698 7, 19 -- for {microscopy-at-microscopy.com} ; Thu, 23 Nov 2006 09:39:03 -0600 7, 19 -- Received: (qmail 87141 invoked by uid 60001); 23 Nov 2006 15:39:02 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 19 -- b=hVWsNBLdPT8OJ+cRYh7ebPum4IIFJrBmR3iGwec0wlKHA9DoHA/QWcz0Ny0TO9FKzCQRrE12i3MJSOdXHJM4OVfZ4rYzpf0z9rcmRh/NyJ3UWHQk9oIbGFPSeiECDSFAEmz9nmscWmfDHnv7NmVV0umhPc2YuFBrC2FUoLNlOJE=; 7, 19 -- X-YMail-OSG: XhFNP5wVM1mltPNRJpdw3q2cRh2TsqpyvuUNWwrM4nNJqo0e9k0iew.rE6kvTC5T8Ni3NYra.tcG2iUuqjZe7gs1DNcPR43b1HKoYNNKJeY93sCBEktlIJhBjuNmsOCDwjsJ2PAXT_qUFfM- 7, 19 -- Received: from [80.122.101.102] by web37415.mail.mud.yahoo.com via HTTP; Thu, 23 Nov 2006 07:39:02 PST 7, 19 -- Date: Thu, 23 Nov 2006 07:39:02 -0800 (PST) 7, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 19 -- Subject: Re: Basic SEM functions 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=iso-8859-1 7, 19 -- Content-Transfer-Encoding: 8bit 7, 19 -- Message-ID: {292322.86779.qm-at-web37415.mail.mud.yahoo.com} ==============================End of - Headers==============================
First off, is there an ion pump at the top of the column for the gun chamber? If there is, the I would assume that the vacuum sequence would be to open the isolation valve and let everything reach terminal vacuum, then close the isolation valve and turn on the gun chamber ion pump to achieve highest vacuum for the gun. If the SEM is of traditional design, it will have one or more differential pumping apertures in the column such that the gun will always be at high vac regardless of what is going on in the chamber. Low vac for the gun will likely kill it quickly.
1. If the EDS detector is tilted, the specimen can be horizontal. The effective take off angle (mechanical tilt + specimen tilt) will affect total counts per second at a particular filter time.
2. Yes. In some cases, it is required. This is a very specimen-specific issue. For example, if you are looking at high Z specimens, then KV needs to be about twice the highest Ma or La eV value. Take Cu for example. It has Ka peak at 8.040KeV and La peak at .930eV. However, Pr has an Ma peak at .929eV. It's unlikely that you would have Cu and Pr but point is that with double peak elements like this example, you would need about 18KV beam to bring up both Cu peaks to ensure Cu is what you have and for the system to quant. A smart system will complain that KV is too low for quant.
On the other hand, if you are looking at light elements like C, O, N, F, Al, these only have one peak of interest--Ka. F is especially tricky since in normal form, it will be burned by a strong beam at high KV. Thus, 5KV is a good value to use to avoid specimen damage, reduce volumetric interaction and still get enough counts to do quant. In IC processing, F is a frequently used gas. It used with Si and Al chucks and is supposed to be in some areas and not in others. If the KV is too high, the very thin layer of F will be overwhelmed by a high KV beam such that the layer is pierced and therefore producing a large peak at Si or Al and likely missing the F.
3. How do you know the beam is 60nm? Apart from this, does the system stigmate properly? Is the gun centered? Is the final aperture centered? Is the condenser lens open or reduced?
As far as SEM resolution is concerned, you have to ask what the expected resolution of "your" SEM should be. Resolution changes from maker-to-maker and model-to-model. It also changes with WD. Shorter WD, better resolution but at the expense of less SEs. FESEMs will have better resolution than thermionic SEMs. There are a whole bunch of variables that affect resolution. Another issue is size of final aperture. Smaller, better but reduced S/N.
Hope this helps.
gary g.
At 07:42 AM 11/23/2006, you wrote:
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==============================Original Headers============================== 15, 22 -- From gary-at-gaugler.com Thu Nov 23 11:17:57 2006 15, 22 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 15, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kANHHupW000751 15, 22 -- for {microscopy-at-microscopy.com} ; Thu, 23 Nov 2006 11:17:56 -0600 15, 22 -- Received: (qmail 12763 invoked from network); 23 Nov 2006 09:17:56 -0800 15, 22 -- Received: by simscan 1.1.0 ppid: 12735, pid: 12761, t: 0.2101s 15, 22 -- scanners: regex: 1.1.0 attach: 1.1.0 15, 22 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 15, 22 -- by qsmtp2 with SMTP; 23 Nov 2006 09:17:56 -0800 15, 22 -- Message-Id: {7.0.1.0.2.20061123084900.024eac80-at-gaugler.com} 15, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 15, 22 -- Date: Thu, 23 Nov 2006 09:17:51 -0800 15, 22 -- To: nizets2-at-yahoo.com 15, 22 -- From: Gary Gaugler {gary-at-gaugler.com} 15, 22 -- Subject: Re: [Microscopy] Re: Basic SEM functions 15, 22 -- Cc: MSA listserver {microscopy-at-microscopy.com} 15, 22 -- In-Reply-To: {200611231542.kANFgDEf023695-at-ns.microscopy.com} 15, 22 -- References: {200611231542.kANFgDEf023695-at-ns.microscopy.com} 15, 22 -- Mime-Version: 1.0 15, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 15, 22 -- Content-Transfer-Encoding: 8bit 15, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kANHHupW000751 ==============================End of - Headers==============================
I have marked my comments against your email below
Steve Chapman Senior Consultant Protrain For electron microscopy consultancy and training world wide Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967 Web www.emcourses.com
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Organization: Max-Planck-Institute of Biochemistry
Title-Subject: [Filtered] How to generate a simple excitation pattern with a fluorescent microscope
Question: Hi, all,
I am working with photoactivatable (ěcagedî) compounds and I am trying to create a simple pattern (a smiley face or for example the letter ëAí) of uncaged compound within a brain slice. This is just supposed to be a prove-of-principle to show the power and spatial resolution of photoactivation. So far, Iíve been using a 10x objective and a DAPI filter set on an old Zeiss Axiophot for uncaging. My idea was to stick a small blackened disc (with air-slits showing the respective pattern) in the field stop of the microscope so that the pattern should in principle be projected onto the brain slice as DAPI light. I had our machine shop make me 3 different discs with slit widths (length:1cm) of 200µm, 600µm, or 1mm to test this idea - but little success. If I use any of these discs to look at a more or less homogenous fluorescent sample, I only see a dimmed image of the entire sample but never a crisp bar of excitation/fluorescence across the specimen. I am wondering now if there is a conceptual mistake in my approach or if I am just not using the proper slits - basically I have no idea whatís going on. So Iíd be thrilled if someone could enlighten me! Also, is there a company out there that could produce discs with a little more elaboration such as a smiley face ? Thanks in advance.
I realize the question basically concerns EDX, but there is also one thing when observing the morphology of a tilted specimen. I ALWAYS advise against the of use the tilt-correction control, or you will get distortion: for example, it makes spheres (which should appear circular when tilted at any angle) come out as ellipsoids, and rectangular blocks will appear as parallelepipeds (lovely word!).
----------------------------------- Robert H. Olley URL: http://www.rdg.ac.uk/~spsolley -----------------------------------
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==============================Original Headers============================== 6, 23 -- From hinmeigeng-at-hotmail.com Fri Nov 24 09:48:37 2006 6, 23 -- Received: from bay0-omc2-s37.bay0.hotmail.com (bay0-omc2-s37.bay0.hotmail.com [65.54.246.173]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAOFmaFY026548 6, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 24 Nov 2006 09:48:36 -0600 6, 23 -- Received: from hotmail.com ([65.55.130.89]) by bay0-omc2-s37.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.1830); 6, 23 -- Fri, 24 Nov 2006 07:48:36 -0800 6, 23 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 6, 23 -- Fri, 24 Nov 2006 07:48:35 -0800 6, 23 -- Message-ID: {BAY125-F93FA77AEBAFE45FDB7E35CAE10-at-phx.gbl} 6, 23 -- Received: from 65.55.130.123 by by125fd.bay125.hotmail.msn.com with HTTP; 6, 23 -- Fri, 24 Nov 2006 15:48:30 GMT 6, 23 -- X-Originating-IP: [134.225.1.161] 6, 23 -- X-Originating-Email: [hinmeigeng-at-hotmail.com] 6, 23 -- X-Sender: hinmeigeng-at-hotmail.com 6, 23 -- Reply-To: R.H.Olley-at-reading.ac.uk 6, 23 -- From: "Robert H. Olley" {hinmeigeng-at-hotmail.com} 6, 23 -- To: Microscopy-at-MSA.Microscopy.Com 6, 23 -- Cc: hinmeigeng-at-hotmail.com, nizets2-at-yahoo.com 6, 23 -- Subject: Basic SEM functions -Tilt 6, 23 -- Date: Fri, 24 Nov 2006 15:48:30 +0000 6, 23 -- Mime-Version: 1.0 6, 23 -- Content-Type: text/plain; format=flowed 6, 23 -- X-OriginalArrivalTime: 24 Nov 2006 15:48:35.0728 (UTC) FILETIME=[00686100:01C70FE0] ==============================End of - Headers==============================
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Email: maloneyb-at-fiu.edu Name: Barbara Maloney
Organization: FIU
Title-Subject: [Filtered] Phillips 300 TEM
Question: Dear Group - I know everyone here in the US is probably on Thanksgiving holiday, but by chance anyone have an idea what year the Phillip 300 TEM was produced? It would be great if one could respond to this query ASAP - also anyone have any idea how many 300s are still operational? Thanks so much. Barbara
The Philips EM 300 was introduced in 1966. Versions of the EM300 with STEM were first sold in 1968.
Information found in "The "History of Electron MIcroscopes - 1986" from 11th International Congress on EM-Kyoto 1986
Nestor Your Friendly Neighborhood SysOp
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==============================Original Headers============================== 8, 13 -- From zaluzec-at-microscopy.com Sat Nov 25 08:14:45 2006 8, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 8, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAPEEjMC005395; 8, 13 -- Sat, 25 Nov 2006 08:14:45 -0600 8, 13 -- Mime-Version: 1.0 8, 13 -- Message-Id: {p06110401c18dff1f705c-at-[206.69.208.22]} 8, 13 -- In-Reply-To: {200611251408.kAPE8ME6032236-at-ns.microscopy.com} 8, 13 -- References: {200611251408.kAPE8ME6032236-at-ns.microscopy.com} 8, 13 -- Date: Sat, 25 Nov 2006 08:14:45 -0600 8, 13 -- To: maloneyb-at-fiu.edu, microscopy-at-microscopy.com 8, 13 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 8, 13 -- Subject: Re: viaWWW: Phillips 300 TEM 8, 13 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
I have accumulated a large number of colloidal gold conjugated antibodies and would like to determine if they are still good without having to screen each in some tedious immunocytochemical staining protocol. I assume one of two things can happen to inactivate an antibody-gold conjugate. The antibody could denature and make it ineffective. Alternatively, the antibody could disassociate from the colloidal gold particle and compete with bound antibody for the binding site which would result in reduced sensitivity. I blotted 50 ng (in 1 ul) of rabbit IgG onto nitrocellulose, blocked for several hours in 1% BSA and then incubated overnight at 4 C in 1:20 dilutions of my stock solutions. Some of my stocks dating back to 1998 still gave nice red dots after an overnight incubation. So at least some of the antibody conjugate is good. If I had been clever I would have tested each of these stocks against a dilution curve of antigen so I could evaluate whether there is reduced sensitivity. But I wasn't clever so now I am stuck trying to interpret which of these stocks are still usable. Does anyone know what level of antigen (i.e., target immunoglobulin) should be detectable by the various size gold particles (sensitivity in seeing blot bound colloidal gold must be linked to gold size). Is seeing a good spot at 50 ng/spot sufficient to proceed? In addition to my colloidal gold stocks, I tested 3 nanogold conjugates which were covalently linked. I naturally couldn't see then after the blot until I gold enhanced but since enhancement gave spots, they must be good since there can't be any dissociation - right? Is the minimally detectable concentration of IgG different for nanogold vs colloidal gold? Isn't this somewhat dependent on the enhancement step? Does anyone know if colloidal gold and/or nanogold conjugates can be kept at -20 C in 50% glycerol (my standard procedure for primary and fluorochrome conjugated antibodies?
I am interested in real world experience from everyone but the knowledgeable comments from Jan Leunissen and Rick Powell would be especially appreciated.
Tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
I am pleased and honored with your request to give you my comments and very much hope others might be willing to share their experiences. Colloid and adsorption physics are a very complicated business and not seldom have a lot of surprises.
In 1991 KRAMARCY and SEALOCK published a paper in JHC Vol. 39, No. 1, pp. 37-39, 1991: "Commercial Preparations of Colloidal Gold-Antibody Complexes Frequently Contain Free Active Antibody" Their data indicate that proteins adsorbed onto colloidal particles of 5nm and larger can dissociate from the particle surface with time and that, at times even shortly after manufacturing, colloidal gold reagents may contain free binding molecules. This is not necessarily the result of bad manufacturing practice as adsorption and desorption are in equilibrium at all times. Some proteins (there are even variations between antibodies from different animal species) are more liable to become dissociated than others and the conditions of coupling play a role as well. If dissociation does occur, than older conjugates will progressively loose activity as a result and this will be partly because of now less active reagent, and secondly because that reagent has now to compete with free (and thermodynamically more favorable) protein in solution.
Whether desorption will occur seems very much to be related to particle size. Ultra small conjugates prepared by adsorption have shown very consistent performance well beyond their shelf life. They do age well....As much as this may be a surprise, it is a pleasant one (at least for the user!) and I guess this illustrates that both adsorption based ultra small conjugates as well as antibodies can have a long and healthy life. Besides adsorption based production of gold reagents the covalent binding principle is used with small organo-metallic gold particles but I am not aware of this approach being used for larger particles.
How to test? What are sensible quality criteria? All producers of colloidal gold reagents (or any particle based immuno reagents for that matter) will have their own in house criteria, some may use additional enhancement even with large 'colored' particles, others may judge the performance exclusively based on the initial color.
So what does this mean in practice? With larger conjugates one should be able to easily detect spots "ŕ vue" when they contain between 1 and 10 ng of specific protein in a dot-spot test under appropriate conditions that comply with the rules of affinity/avidity binding. Using enhancement the levels may go down into the picogram range. If you (or anyone else) should be interested, we have a newsletter available by Peter van de Plas describing in detail how to perform simple tests that demonstrate activity and performance of gold conjugates, primary antibodies and enhancement reagents, even down to the level of antigen recognition.
Storing conjugates....whenever one removes water from a hydrophilic structure that has partly hydrophobic qualities (as antibodies do) the risk of clustering based on hydrophobic interactions will definitely increase. And especially with conjugates built around large gold particles London/van der Waals interactions come into play as well. In case I would find myself at some point with a lot of conjugate left that was still reasonably 'fresh', I would store it at -20°C as a liquid (without freezing!) as you suggest; maybe rather use sucrose, as glycerol may affect membranes. I have no solid data for this but generally speaking desorption will be much reduced with lower temperatures, and should any clustering result from this treatment, a short spin of a ready-to-use diluted conjugate in a microfuge may remove such clusters.
Some of the above is based on our in-house or personal experience and may not necessarily have been publicly documented. Hope the info is helpful nevertheless. And finally: in spite of all the criteria I mentioned: sometimes old conjugates do remarkably well, even though their performance in a dot spot test would make one expect them to fail. I would say: if possible it is always worth a try with a well know test specimen and primary antibody combination.
Good luck!
Jan Leunissen
Aurion - President Electron Microscopy Research Advisor Costerweg 5 Dept Anatomy and Structural Biology 6702 A Wageningen Otago School of Medicine The Netherlands Dunedin, New Zealand phone 31-317-497676 phone 64-3-4795465 fax 31-317-415955 fax 64-3-4797254 http://www.aurion.nl http://anatomy.otago.ac.nz
==============================Original Headers============================== 16, 21 -- From leunissen-at-aurion.nl Sun Nov 26 19:42:07 2006 16, 21 -- Received: from fep04.xtra.co.nz (fep04.xtra.co.nz [210.54.141.242]) 16, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAR1g52q028898 16, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 26 Nov 2006 19:42:06 -0600 16, 21 -- Received: from [192.168.1.50] (really [222.152.239.41]) by fep04.xtra.co.nz 16, 21 -- with ESMTP 16, 21 -- id {20061127014611.VYRD19621.fep04.xtra.co.nz-at-[192.168.1.50]} ; 16, 21 -- Mon, 27 Nov 2006 14:46:11 +1300 16, 21 -- In-Reply-To: {200611251919.kAPJJv2F029976-at-ns.microscopy.com} 16, 21 -- References: {200611251919.kAPJJv2F029976-at-ns.microscopy.com} 16, 21 -- Mime-Version: 1.0 (Apple Message framework v752.2) 16, 21 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 16, 21 -- Message-Id: {0505EFBA-F4F2-463D-BF56-E58660DAF0BE-at-aurion.nl} 16, 21 -- Cc: Microscopy-at-microscopy.com 16, 21 -- From: Jan Leunissen {leunissen-at-aurion.nl} 16, 21 -- Subject: Re: [Microscopy] colloidal gold stability 16, 21 -- Date: Mon, 27 Nov 2006 14:46:10 +1300 16, 21 -- To: Tom Phillips {phillipst-at-missouri.edu} 16, 21 -- X-Mailer: Apple Mail (2.752.2) 16, 21 -- Content-Transfer-Encoding: 8bit 16, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAR1g52q028898 ==============================End of - Headers==============================
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Email: neurowu-at-yahoo.com Name: mike
Organization: NDT
Title-Subject: [Filtered] price of used ccd camera
Question: Hi,
we are looking for a pre-owned ccd camera, such as optronics, Qimaging, Cooke, Roper, etc that can support our brightfield and/or fluorescent digital imagaing and quantitative analysis (volumentric analysis and cell, spine counting). Due to our budget limit, we can only offer less than USD1500. Please let me know if you have available one for sale or know the right party we can contact with, thanks!
Have you thought of an Astronomical camera? $1500 is tight, but it might cover an Artemis camera. I'd recommend one of the better Sony chips, e.g. ICX285AL or ICX285AQ. http://www.artemisccd.co.uk/icx285c.htm http://www.atik-instruments.com/
Good luck,
Austin
P.S. I'm not financially involved with Artemis; but I do have one of their excellent cameras.
----- Original Message ----- X-from: {neurowu-at-yahoo.com} To: {AuntDaisy-at-gmail.com} Sent: Monday, November 27, 2006 9:35 AM
Dear fellow microscopers,
Once again the efficiency of this list is breathtaking and I warmly thank all of you who spent some time explaining me the basics of SEM. I learned a lot in a short time and, though I had absolutely no experience in SEM, I can now run the microscope and even do EDX analysis (and EDX mapping! but this is thanks to the software). It goes without saying that I will need the help of an engineer or more experienced user to take the best of the instrument, but in the meantime I can get used to its manipulation.
Stephane
--- jmastrangelo-at-ultralifebatteries.com wrote:
} Hi Stephane, } } We purchased a TESCAN VEGAII last year, so I am by } no means an expert user } yet. Your problems with blurriness over 2Kx } magnification do sound familiar } to me, though. I was struggling with the same issues } when I was first } learning how to use the instrument. Have you had } training in the alignment / } beam optimization procedures yet? A few months after } the service tech } installed our instrument, TESCAN sent one of their } applications experts here } to our facilities for a couple days of training on } the instrument. He gave } me an alignment procedure that has greatly increased } the resolution in my } images. There is a lot of fine tuning to be done } with beam alignment and } with the stigmators to get good resolution at high } magnification. I do not } work at extremely high magnification but I have } acquired good images in the } 50-70Kx range. I have been able to resolve and image } particles of about } 20nm, although I believe the TESCAN people say the } instrument is good to } about 5nm for resolution. } } Hope this helps. If you like, I can send you the } general procedure I use to } optimize the imaging, although as I said- I am by no } means an expert user. } The guy at TESCAN that has been the greatest help to } me is Jack Mershon. He } could help you with more technical questions. Good } luck! } } Regards, } } ---------------------------------------- } Joe Mastrangelo } Chemical Lab Technician } Ultralife Batteries } 2000 Technology Parkway } Newark, NY 14513 } USA } } E-mail: jmastrangelo-at-ulbi.com } Phone: 315-359-6203 } Fax: 315-331-4264 } www.ultralifebatteries.com } } We. Are. Power. } } Want news, knowledge and commentary } from Ultralife experts? } Sign up for our Newsletter. } Subscribe at: www.ulbi.com/sign_up.php } } } } } } } -----Original Message----- } } From: nizets2-at-yahoo.com [SMTP:nizets2-at-yahoo.com] } } Sent: Thursday, November 23, 2006 10:48 AM } } To: jmastrangelo-at-ultralifebatteries.com } } Subject: [Microscopy] Re: Basic SEM functions } } } } } } } } } } } -------------------------------------------------------------------------- } } -- } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } -------------------------------------------------------------------------- } } -- } } } } I finally found the necessary sequence to have } "green } } light" for "high vacuum" mode. Funnily, the vacuum } } remains the same, but now I have a green signal } and I } } can activate the HT and filament! } } Here are some few more questions: } } } } } } 1. In TEM one needs to tilt the sample to improve } the } } signal by EDX. Is it the same in SEM? (considering } } that the detector is also at an angle above the } } sample) } } 2. Is it realistic to use HT of 5kV or 10kV to } take } } EDX spectra by SEM? } } 3. Resolution issue: while observing mineral } } particules (quarz for example), when I reach a } } magnification of 2kx or higher (to observe } particles } } several µm big for example), the image is not } sharp. } } Using the mode which offers the maximum resolution } } (cleverly called "resolution mode"), the beam } reaches } } a size of 60nm. In these conditions it is not } } surprising that the image is not sharp. The SEM } allows } } magnification of up to 500kx!! What is the point } to } } use so high magnification if the image is blurred? } } What is the expected resolution of a SEM? } } } } Stephane } } } } } } } } } } } __________________________________________________________________________ } } __________ } } Do you Yahoo!? } } Everyone is raving about the all-new Yahoo! Mail } beta. } } http://new.mail.yahoo.com } } } } ==============================Original } } Headers============================== } } 7, 19 -- From nizets2-at-yahoo.com Thu Nov 23 } 09:39:03 2006 } } 7, 19 -- Received: from } web37415.mail.mud.yahoo.com } } (web37415.mail.mud.yahoo.com [209.191.91.147]) } } 7, 19 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with SMTP id } } kANFd2jH020698 } } 7, 19 -- for {microscopy-at-microscopy.com} ; Thu, 23 } Nov 2006 09:39:03 } } -0600 } } 7, 19 -- Received: (qmail 87141 invoked by uid } 60001); 23 Nov 2006 } } 15:39:02 -0000 } } 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; } c=nofws; } } 7, 19 -- s=s1024; d=yahoo.com; } } 7, 19 -- } } } h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Cont } } ent-Transfer-Encoding:Message-ID; } } 7, 19 -- } } } b=hVWsNBLdPT8OJ+cRYh7ebPum4IIFJrBmR3iGwec0wlKHA9DoHA/QWcz0Ny0TO9FKzCQRrE12 } } } i3MJSOdXHJM4OVfZ4rYzpf0z9rcmRh/NyJ3UWHQk9oIbGFPSeiECDSFAEmz9nmscWmfDHnv7Nm } } VV0umhPc2YuFBrC2FUoLNlOJE=; } } 7, 19 -- X-YMail-OSG: } } } XhFNP5wVM1mltPNRJpdw3q2cRh2TsqpyvuUNWwrM4nNJqo0e9k0iew.rE6kvTC5T8Ni3NYra.t } } } cG2iUuqjZe7gs1DNcPR43b1HKoYNNKJeY93sCBEktlIJhBjuNmsOCDwjsJ2PAXT_qUFfM- } } 7, 19 -- Received: from [80.122.101.102] by } web37415.mail.mud.yahoo.com } } via HTTP; Thu, 23 Nov 2006 07:39:02 PST } } 7, 19 -- Date: Thu, 23 Nov 2006 07:39:02 -0800 } (PST) } } 7, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} } } 7, 19 -- Subject: Re: Basic SEM functions } } 7, 19 -- To: microscopy-at-microscopy.com } } 7, 19 -- MIME-Version: 1.0 } } 7, 19 -- Content-Type: text/plain; } charset=iso-8859-1 } } 7, 19 -- Content-Transfer-Encoding: 8bit } } 7, 19 -- Message-ID: } {292322.86779.qm-at-web37415.mail.mud.yahoo.com} } } ==============================End of - } } Headers============================== } } This email was scanned for viruses before leaving } the Ultralife Batteries, Inc. network. }
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==============================Original Headers============================== 11, 20 -- From nizets2-at-yahoo.com Mon Nov 27 09:34:48 2006 11, 20 -- Received: from web37407.mail.mud.yahoo.com (web37407.mail.mud.yahoo.com [209.191.91.139]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kARFYl1P016224 11, 20 -- for {microscopy-at-microscopy.com} ; Mon, 27 Nov 2006 09:34:47 -0600 11, 20 -- Received: (qmail 87231 invoked by uid 60001); 27 Nov 2006 15:34:46 -0000 11, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 20 -- s=s1024; d=yahoo.com; 11, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 11, 20 -- b=voxU3m4hEY08OVlwP6we+6HuVt7zCnElMbwksO2zAT0f+eBi+DTbSBVoXHzEHxg09pexxmoDKS+p9qkdcwsOMFFYcgMR2fzg/Nn34zRZzWG+4bpRQhKJoqbeQVxi+wu/azuKU7CDpHvGf5G+G+smkhpmiTMvU7arK8zXV3CgYB8=; 11, 20 -- X-YMail-OSG: 3ZZwG1cVM1lW482zaEfijpCY7KNryRqOGtikNSdlWdDPj76B0avT0EVO9Ic7Lwr9wKx9u.2eGWAVY5LMsgcSyqNgeVvIez_aIQzOWih_9s2850AnHXHckOLaB.gi3MhVu.PIxOJyXgrcJAEr5IJd94b35Y4JHT4QBoIqQQpVgICDBbXEyWTeddKuc_Zi2PZtmyG405Af 11, 20 -- Received: from [80.122.101.102] by web37407.mail.mud.yahoo.com via HTTP; Mon, 27 Nov 2006 07:34:46 PST 11, 20 -- Date: Mon, 27 Nov 2006 07:34:46 -0800 (PST) 11, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 11, 20 -- Subject: RE: [Microscopy] Re: Basic SEM functions - thank you 11, 20 -- To: microscopy-at-microscopy.com 11, 20 -- In-Reply-To: {A0C02A449BCBD311AEA10008C75C2A6C0856C1EB-at-PM_GENERAL} 11, 20 -- MIME-Version: 1.0 11, 20 -- Content-Type: text/plain; charset=iso-8859-1 11, 20 -- Content-Transfer-Encoding: 8bit 11, 20 -- Message-ID: {629664.87163.qm-at-web37407.mail.mud.yahoo.com} ==============================End of - Headers==============================
I am sorry if my email behavior caused anybody any discomfort. I don't understand why it is so, I don't understand what I do wrong and I cannot change it(sorry I am no email expert). All I can propose is to stop emailing me if you think that I do something wrong.
Stephane
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==============================Original Headers============================== 4, 20 -- From nizets2-at-yahoo.com Mon Nov 27 09:43:56 2006 4, 20 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kARFhtFw027246 4, 20 -- for {microscopy-at-microscopy.com} ; Mon, 27 Nov 2006 09:43:55 -0600 4, 20 -- Received: (qmail 37439 invoked by uid 60001); 27 Nov 2006 15:43:54 -0000 4, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 4, 20 -- s=s1024; d=yahoo.com; 4, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 4, 20 -- b=YuTFgvVAKgVH35/oOyFd1d83gVCYmpo5ES3B7lHZWqZhc5n/2TGGcHINOHeexesYlXrd31vAlGBmm/cXlc+V8UsGerEC/wM95Dbc7Tw5v+LpFJqL3OEJ40NGJc3j5CU/7YKa1w3bUFi0h6uALmUAw2M/u2WXdEApos5vd22p7Uo=; 4, 20 -- X-YMail-OSG: C1fKULEVM1kJ1ua5E47_75D8cGaWdcCXahIQREc2xGShe1.ptbPi6shtQriNq_lYmpfV8otvMpvHFYqxo7gmeExlaSBA7KHdaPc2xWPrjH2Ccg90iicrf2p.isKYrW0rT0tRxhHqH16PAw-- 4, 20 -- Received: from [80.122.101.102] by web37405.mail.mud.yahoo.com via HTTP; Mon, 27 Nov 2006 07:43:54 PST 4, 20 -- Date: Mon, 27 Nov 2006 07:43:54 -0800 (PST) 4, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 4, 20 -- Subject: Re: [Microscopy] Re: Basic SEM functions 4, 20 -- To: microscopy-at-microscopy.com 4, 20 -- In-Reply-To: {200611252217.kAPMHOt29404-at-www.matscieng.sunysb.edu} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: text/plain; charset=iso-8859-1 4, 20 -- Content-Transfer-Encoding: 8bit 4, 20 -- Message-ID: {688158.37253.qm-at-web37405.mail.mud.yahoo.com} ==============================End of - Headers==============================
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Email: rpowell-at-nanoprobes.com Name: Rick Powell
I'm also honored to be asked...our experience with conventional colloidal gold is a little less than Jan's, but from our experience, your test is a good one for conjugates of 5, 10 and 15 nm colloidal gold, where 50 ng of target gives a reasonably clear red spot (we obtain progressively lighter spots down to about 5 or 10 ng, sometimes 1 ng for 15 nm gold, for good preps); the spot will be darker for larger colloidal gold particles. A good deep red spot would be a good indication of a working conjugate.
With NanogoldĆ, for product testing purposes, we consider clear detection of 10 ng of target after silver enhancement to be acceptable (In practice, we can usually detect 0.1, and frequently 0.01 ng of target after silver enhancement). 50 ng of target is a little more than I would have chosen as a test amount - i.e. it may be enough to give you a signal even if the antibody has lost some native affinity (I would suggest 1 or 5 ng). However, as with colloidal gold, the spots become progressively lighter with smaller amounts of target, and if you compare your blots with one that shows how the spots look for a series of dilutions of target, it will give you a good qualitative idea of how good your conjugates are.
Our web site has a photo of a blot where we used Nanogold-labeled Fab' anti-mouse to detect serial dilutions of mouse IgG:
http://www.nanoprobes.com/NanoAb.html#blot
The first (darkest) pair of spots (top left) contain 10 ng of target IgG, reading left to right along the top row, the second pair contains 5 ng, and the third 1 ng; if your 50 ng spots look like any of these three after you develop them with gold enhancement, the conjugates are definitely still good. After silver or gold enhancement, the spots should be black (sometimes purplish, sometimes brownish) rather than deep red.
More images are available in Hainfeld and Furuya's paper describing Nanogold (Hainfeld, J.F. and Furuya, F.R. A 1.4nm Gold cluster covalently attached to antibodies improves immunolabeling, J. Histochem. Cytochem., 40, 177-184 (1992)); PDF reprint available at http://www.jhc.org/cgi/reprint/40/2/177.
We don't usually freeze Nanogold conjugates, but freezing at -20C in 30% glycerol is usually recommended to avoid ice crystal damage, and your procedure with 50% glycerol should be equally effective. Provided they are not allowed to dry out or become contaminated, Nanogold conjugates can last well beyond the year's shelf life if they are refrigerated at 4C; we have tested them after two or three years and found blot detection sensitivity to be unchanged (we include a bacterial inhibitor).
Hope this helps,
Rick Powell Nanoprobes, Incorporated www.nanoprobes.com
I have accumulated a large number of colloidal gold conjugated antibodies and would like to determine if they are still good without having to screen each in some tedious immunocytochemical staining protocol. I assume one of two things can happen to inactivate an antibody-gold conjugate. The antibody could denature and make it ineffective. Alternatively, the antibody could disassociate from the colloidal gold particle and compete with bound antibody for the binding site which would result in reduced sensitivity. I blotted 50 ng (in 1 ul) of rabbit IgG onto nitrocellulose, blocked for several hours in 1% BSA and then incubated overnight at 4 C in 1:20 dilutions of my stock solutions. Some of my stocks dating back to 1998 still gave nice red dots after an overnight incubation. So at least some of the antibody conjugate is good. If I had been clever I would have tested each of these stocks against a dilution curve of antigen so I could evaluate whether there is reduced sensitivity. But I wasn't clever so now I am stuck trying to interpret which of these stocks are still usable. Does anyone know what level of antigen (i.e., target immunoglobulin) should be detectable by the various size gold particles (sensitivity in seeing blot bound colloidal gold must be linked to gold size). Is seeing a good spot at 50 ng/spot sufficient to proceed? In addition to my colloidal gold stocks, I tested 3 nanogold conjugates which were covalently linked. I naturally couldn't see then after the blot until I gold enhanced but since enhancement gave spots, they must be good since there can't be any dissociation - right? Is the minimally detectable concentration of IgG different for nanogold vs colloidal gold? Isn't this somewhat dependent on the enhancement step? Does anyone know if colloidal gold and/or nanogold conjugates can be kept at -20 C in 50% glycerol (my standard procedure for primary and fluorochrome conjugated antibodies?
I am interested in real world experience from everyone but the knowledgeable comments from Jan Leunissen and Rick Powell would be especially appreciated.
Tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
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==============================Original Headers============================== 15, 20 -- From MCarlyle-at-veeco.com Mon Nov 27 13:26:34 2006 15, 20 -- Received: from sboexch2.int.veeco.com (SBOEXCH2.VEECO.com [68.111.35.167]) 15, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kARJQXj2022562 15, 20 -- for {Microscopy-at-Microscopy.Com} ; Mon, 27 Nov 2006 13:26:33 -0600 15, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 15, 20 -- content-class: urn:content-classes:message 15, 20 -- MIME-Version: 1.0 15, 20 -- Content-Type: text/plain; 15, 20 -- charset="iso-8859-1" 15, 20 -- Subject: AFM -- Seeing at the Nanoscale V Conference Call for Papers 15, 20 -- Date: Mon, 27 Nov 2006 11:26:32 -0800 15, 20 -- Message-ID: {625BD6DD96DE554DBB0558FFE70E0F4201AD58B2-at-sboexch2.int.veeco.com} 15, 20 -- X-MS-Has-Attach: 15, 20 -- X-MS-TNEF-Correlator: 15, 20 -- Thread-Topic: AFM -- Seeing at the Nanoscale V Conference Call for Papers 15, 20 -- Thread-Index: AccSWfHhVuXmyKsVTfiPxBebYfBLrw== 15, 20 -- From: "Marlene Carlyle" {MCarlyle-at-veeco.com} 15, 20 -- To: {Microscopy-at-Microscopy.Com} 15, 20 -- Content-Transfer-Encoding: 8bit 15, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kARJQXj2022562 ==============================End of - Headers==============================
Sometimes I want to get rid of mucous, but sometimes I want to preserve it. In this case the researchers want to look at microbes in the mucous surrounding corals, as well as microbes within the coral tissue with TEM. I have had pretty good success decalcifying corals for TEM, but it's been a long time since I wanted to keep slime. I know people use ruthenium red and Alcian blue to stain mucous, but do these preserve it throughout processing for TEM as well? I also have some ruthenium tetroxide in the fridge from the early 1990s; I think it was once used for preserving slimy biofilms.
Any suggestions welcomed!
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 6, 19 -- From tina-at-pbrc.hawaii.edu Mon Nov 27 19:35:11 2006 6, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAS1ZA94010133 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 27 Nov 2006 19:35:10 -0600 6, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id kAS1Z6x4011562 6, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 27 Nov 2006 15:35:06 -1000 (HST) 6, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id kAS1Z5B2011559 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 27 Nov 2006 15:35:05 -1000 (HST) 6, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 6, 19 -- Date: Mon, 27 Nov 2006 15:35:04 -1000 (HST) 6, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 19 -- X-Sender: tina-at-halia 6, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 6, 19 -- Subject: Preservation of microbes in external mucous 6, 19 -- Message-ID: {Pine.GSO.4.21.0611271530510.11499-100000-at-halia} 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
I am looking for a database for atomic positions (Wyckoff Positions ) for compounds (metals, oxides and ceramics) and software to construct structures to view planes, different sites (octahedral, tetrahedral, etc.), and diffraction simulation. Also any recommendations for a good book in practical electron microscopy which includes the new microscopy techniques. I would appreciate any information on the above issues. Thank you,
Can anyone point me to the specification of the Cambridge S90 SEM and when the machine was first marketed? I tried google but nothing relevant came uo. Thanks a lot.
-- Pang (Wai Pang Chan, wpchan-at-u.washington.edu, PAB A087, 206-685-1519) The Biology Imaging Facility (http://staff.washington.edu/wpchan/if/)
==============================Original Headers============================== 2, 20 -- From wpchan-at-u.washington.edu Mon Nov 27 22:54:41 2006 2, 20 -- Received: from mxout5.cac.washington.edu (mxout5.cac.washington.edu [140.142.32.135]) 2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAS4sfku003095 2, 20 -- for {microscopy-at-microscopy.com} ; Mon, 27 Nov 2006 22:54:41 -0600 2, 20 -- Received: from homer22.u.washington.edu (homer22.u.washington.edu [140.142.15.9]) 2, 20 -- by mxout5.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW06.09) with ESMTP id kAS4se9L018878 2, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 2, 20 -- for {microscopy-at-microscopy.com} ; Mon, 27 Nov 2006 20:54:40 -0800 2, 20 -- Received: from localhost (wpchan-at-localhost) 2, 20 -- by homer22.u.washington.edu (8.13.7+UW06.06/8.13.7+Submit) with ESMTP id kAS4seWC032644 2, 20 -- for {microscopy-at-microscopy.com} ; Mon, 27 Nov 2006 20:54:40 -0800 2, 20 -- Date: Mon, 27 Nov 2006 20:54:40 -0800 (PST) 2, 20 -- From: "W. Chan" {wpchan-at-u.washington.edu} 2, 20 -- To: microscopy-at-microscopy.com 2, 20 -- Subject: cambridge S90 2, 20 -- Message-ID: {Pine.LNX.4.64.0611272015270.28974-at-homer22.u.washington.edu} 2, 20 -- MIME-Version: 1.0 2, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 2, 20 -- X-PMX-Version: 5.2.2.285561, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2006.11.27.204432 2, 20 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
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Email: brunnerrobert-at-netzero.com Name: Robert Brunner
Organization: Surpass Inc.
Title-Subject: [Filtered] Tissue Prep and hold for TEM and or SEM
Question: I am working with a Scientist that wants to collect tissue from a study animal to hold for potential SEM and or TEM. I was wondering if anyone can provide any procedures for preparation of the tissue from collection to fixation to storage that would give optimal results for future SEM and TEM studies. It is not known how long the samples will be stored so please let me know if there is a time limit on the storage of the samples also.
I have found the ICSD database useful. You may have to subscribe first. The database is pretty complete and I've found it quite useful. See:
http://icsdweb.fiz-karlsruhe.de/index.php
The other place to look is Pearson's Handbook of Crystallographic Data for Intermetallic Phases(edited by Villars and Calvert), which is a particularly good reference for metals but is only partial for oxides.
Regards, Wharton
-----Original Message----- X-from: jafarhan-at-rci.rutgers.edu [mailto:jafarhan-at-rci.rutgers.edu] Sent: Monday, November 27, 2006 8:15 PM To: Sinkler, Wharton
Dear folks,
I am looking for a database for atomic positions (Wyckoff Positions ) for compounds (metals, oxides and ceramics) and software to construct structures to view planes, different sites (octahedral, tetrahedral, etc.), and diffraction simulation. Also any recommendations for a good book in practical electron microscopy which includes the new microscopy techniques. I would appreciate any information on the above issues. Thank you,
What tissue? What type of study? Can the tissues be freeze-fixed? I collected and processed tissues in Antarctica for study back in Chicago, but my methods may not be relevant to the studies. And given that one of the trips was done in the days of Pel-Dri, before it became illegal, that method is definitely not relevant. Hm. How does the tissue work with tert-butyl alcohol? Might try dehydrating with that, and then letting the tBA freeze (pretty much freezes at room temp.), and storing that way. I've never tried this and have no idea if it would work, but tBA has been used for light microscopy, and as a dehydrating/freeze-sublimation drying method for SEM (there's a reference for this, but I haven't found it in my horizontal filiing system). If the tissues can be freeze-fixed, a possible method would be EM freeze-fixation and storage well below the recrystallization temperature (I'd use -90 C or better) then careful rewarming and freeze-substitution. But again, I haven't tried that for storage.
Phil
} This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both brunnerrobert-at-netzero.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: brunnerrobert-at-netzero.com } Name: Robert Brunner } } Organization: Surpass Inc. } } Title-Subject: [Filtered] Tissue Prep and hold for TEM and or SEM } } Question: I am working with a Scientist that wants to collect tissue } from a study animal to hold for potential SEM and or TEM. I was } wondering if anyone can provide any procedures for preparation of the } tissue from collection to fixation to storage that would give optimal } results for future SEM and TEM studies. It is not known how long the } samples will be stored so please let me know if there is a time limit } on the storage of the samples also. } } Thanks for any help } } } Robert Brunner -- Philip Oshel Microscopy Facility Supervisor Biology Department Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462
==============================Original Headers============================== 3, 23 -- From oshel1pe-at-cmich.edu Tue Nov 28 07:52:35 2006 3, 23 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kASDqZaD014715 3, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Nov 2006 07:52:35 -0600 3, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 3, 23 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id kASEM2N4004123; 3, 23 -- Tue, 28 Nov 2006 09:22:09 -0500 3, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 3, 23 -- Tue, 28 Nov 2006 08:52:25 -0500 3, 23 -- Mime-Version: 1.0 3, 23 -- Message-Id: {f06230918c191eab08cbb-at-[141.209.160.249]} 3, 23 -- In-Reply-To: {200611280802.kAS82uiZ024523-at-ns.microscopy.com} 3, 23 -- References: {200611280802.kAS82uiZ024523-at-ns.microscopy.com} 3, 23 -- Date: Tue, 28 Nov 2006 08:52:24 -0500 3, 23 -- To: brunnerrobert-at-netzero.com 3, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 23 -- Subject: Re: [Microscopy] viaWWW: Tissue Prep and hold for TEM and or SEM 3, 23 -- Cc: Microscopy-at-microscopy.com 3, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 23 -- X-OriginalArrivalTime: 28 Nov 2006 13:52:25.0790 (UTC) FILETIME=[6FA73DE0:01C712F4] 3, 23 -- X-CanItPRO-Stream: default 3, 23 -- X-Spam-Score: -4 () L_EXCH_MF 3, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
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Title-Subject: [Filtered] preparation of nanowires for TEM
Question: I have got ZnSe nanowires grown on a GaAs substrate. Up to now, I have prepared them by putting a piece of the sample in methanol and letting it for one hour in an ultrasonic device ; them I put a drop of the solution on a copper carbon grid. With this method, the nanowires are broken and one cannot study the interface with the substrate. This is why I am trying to find another method of preparation keeping them on the substrate.
Besides the things you mentioned, there are techniques for "non-aqueous" fixation to preserve biofilms using fluorocarbon solvents, such as Fluorinert FC-72, which is manufactured by the 3M company. Call them for distributor information. Also high-pressure freezing and freeze-substitution are options, and dehydration in HMDS can help retain mucins for SEM studies.
References to check:
P. Allan-Wojtas et al., Micros. Res. Tech., 36:390-399 (1997)
Sims, DE et al. Biotech Histochem. 66(4):173-80 (1991)
Sanchez et al. J Comp Path. 117(2):165-70 (1997)
Sims and Horne Am J Physiol 273: L1036-L1041 (1997)
Bock et al. Biotech Histochem 74(5):244-7 (1999)
There....that ought to get you started. Hope it helps!
65 and muggy in Columbia.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: tina-at-pbrc.hawaii.edu [mailto:tina-at-pbrc.hawaii.edu] Sent: Monday, November 27, 2006 7:37 PM To: Tindall, Randy D.
Hi, All-
Sometimes I want to get rid of mucous, but sometimes I want to preserve it. In this case the researchers want to look at microbes in the mucous surrounding corals, as well as microbes within the coral tissue with TEM. I have had pretty good success decalcifying corals for TEM, but it's been a long time since I wanted to keep slime. I know people use ruthenium red and Alcian blue to stain mucous, but do these preserve it throughout processing for TEM as well? I also have some ruthenium tetroxide in the fridge from the early 1990s; I think it was once used for preserving slimy biofilms.
Any suggestions welcomed!
Aloha, Tina
************************************************************************ **** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ************************************************************************ ****
==============================Original Headers============================== 6, 19 -- From tina-at-pbrc.hawaii.edu Mon Nov 27 19:35:11 2006 6, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAS1ZA94010133 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 27 Nov 2006 19:35:10 -0600 6, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id kAS1Z6x4011562 6, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 27 Nov 2006 15:35:06 -1000 (HST) 6, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id kAS1Z5B2011559 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 27 Nov 2006 15:35:05 -1000 (HST) 6, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 6, 19 -- Date: Mon, 27 Nov 2006 15:35:04 -1000 (HST) 6, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 19 -- X-Sender: tina-at-halia 6, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 6, 19 -- Subject: Preservation of microbes in external mucous 6, 19 -- Message-ID: {Pine.GSO.4.21.0611271530510.11499-100000-at-halia} 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
==============================Original Headers============================== 27, 25 -- From TindallR-at-missouri.edu Tue Nov 28 08:54:49 2006 27, 25 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 27, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kASEsnKW005045 27, 25 -- for {microscopy-at-microscopy.com} ; Tue, 28 Nov 2006 08:54:49 -0600 27, 25 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 27, 25 -- Tue, 28 Nov 2006 08:54:48 -0600 27, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5 27, 25 -- Content-class: urn:content-classes:message 27, 25 -- MIME-Version: 1.0 27, 25 -- Content-Type: text/plain; 27, 25 -- charset="us-ascii" 27, 25 -- Subject: RE: [Microscopy] Preservation of microbes in external mucous 27, 25 -- Date: Tue, 28 Nov 2006 08:54:48 -0600 27, 25 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68C03-at-UM-XMAIL08.um.umsystem.edu} 27, 25 -- In-Reply-To: {200611280136.kAS1aqD6012158-at-ns.microscopy.com} 27, 25 -- X-MS-Has-Attach: 27, 25 -- X-MS-TNEF-Correlator: 27, 25 -- Thread-Topic: [Microscopy] Preservation of microbes in external mucous 27, 25 -- Thread-Index: AccSlDWQ1XmlUiCcSPOIQrO5ufSEGwAZIn2Q 27, 25 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 27, 25 -- To: {tina-at-pbrc.hawaii.edu} 27, 25 -- Cc: {microscopy-at-microscopy.com} 27, 25 -- X-OriginalArrivalTime: 28 Nov 2006 14:54:48.0039 (UTC) FILETIME=[26351F70:01C712FD] 27, 25 -- Content-Transfer-Encoding: 8bit 27, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kASEsnKW005045 ==============================End of - Headers==============================
A group in the Geology Department at Arizona State University maintains an internet site for the American Mineralogist Crystal Structure Database. It's free, contains atomic position data for most minerals and has useful information not in Pearson's Handbook. The address is rruff.geo.arizona.edu/AMS/amcsd.php.
Larry Thomas PNNL
On 11/28/06 5:23 AM, "Wharton.Sinkler-at-uop.com" {Wharton.Sinkler-at-uop.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Jafar, } } I have found the ICSD database useful. You may have to subscribe first. } The database is pretty complete and I've found it quite useful. See: } } http://icsdweb.fiz-karlsruhe.de/index.php } } The other place to look is Pearson's Handbook of Crystallographic Data } for Intermetallic Phases(edited by Villars and Calvert), which is a } particularly good reference for metals but is only partial for oxides. } } Regards, } Wharton } } -----Original Message----- } X-from: jafarhan-at-rci.rutgers.edu [mailto:jafarhan-at-rci.rutgers.edu] } Sent: Monday, November 27, 2006 8:15 PM } To: Sinkler, Wharton } Subject: [Microscopy] Atomic positions database and software } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } } Dear folks, } } I am looking for a database for atomic positions (Wyckoff Positions ) } for } compounds (metals, oxides and ceramics) and software to construct } structures to view planes, different sites (octahedral, tetrahedral, } etc.), and diffraction simulation. Also any recommendations for a good } book in practical electron microscopy which includes the new microscopy } techniques. } I would appreciate any information on the above issues. } Thank you, } } Jafar } } Materials Science and Engineering } Rutgers University, } Piscataway, NJ 08854 } Tel. 732 445 5615 } } ==============================Original } Headers============================== } 5, 21 -- From jafarhan-at-rci.rutgers.edu Mon Nov 27 20:13:28 2006 } 5, 21 -- Received: from rci.rutgers.edu (rci-out.rutgers.edu } [128.6.68.135]) } 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id kAS2DSdR021489 } 5, 21 -- for {microscopy-at-microscopy.com} ; Mon, 27 Nov 2006 } 20:13:28 -0600 } 5, 21 -- Received: by rci.rutgers.edu (Postfix, from userid 11335) } 5, 21 -- id DE1BA12D6; Mon, 27 Nov 2006 21:13:27 -0500 (EST) } 5, 21 -- Received: from 68.192.253.101 } 5, 21 -- (SquirrelMail authenticated user jafarhan) } 5, 21 -- by webmail.rci.rutgers.edu with HTTP; } 5, 21 -- Mon, 27 Nov 2006 21:13:27 -0500 (EST) } 5, 21 -- Message-ID: } {3589.68.192.253.101.1164680007.squirrel-at-webmail.rci.rutgers.edu} } 5, 21 -- Date: Mon, 27 Nov 2006 21:13:27 -0500 (EST) } 5, 21 -- Subject: Atomic positions database and software } 5, 21 -- From: jafarhan-at-rci.rutgers.edu } 5, 21 -- To: microscopy-at-microscopy.com } 5, 21 -- User-Agent: SquirrelMail/1.4.7 } 5, 21 -- MIME-Version: 1.0 } 5, 21 -- Content-Type: text/plain;charset=iso-8859-1 } 5, 21 -- Content-Transfer-Encoding: 8bit } 5, 21 -- X-Priority: 3 (Normal) } 5, 21 -- Importance: Normal } ==============================End of - } Headers============================== } } } ==============================Original Headers============================== } 16, 27 -- From Wharton.Sinkler-at-uop.com Tue Nov 28 07:18:21 2006 } 16, 27 -- Received: from AZ18CN849.global.ds.honeywell.com } (tmpnat1.honeywell.com [199.64.0.252]) } 16, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } kASDIKln003069 } 16, 27 -- for {microscopy-at-microscopy.com} ; Tue, 28 Nov 2006 07:18:21 -0600 } 16, 27 -- Received: from dplexch7.uop.com ([138.90.2.112]) by } AZ18CN849.global.ds.honeywell.com with Microsoft SMTPSVC(6.0.3790.1830); } 16, 27 -- Tue, 28 Nov 2006 06:18:20 -0700 } 16, 27 -- Received: from DPLEVS1.ad.uop.com ([138.90.2.100]) by } dplexch7.uop.com with Microsoft SMTPSVC(6.0.3790.1830); } 16, 27 -- Tue, 28 Nov 2006 07:18:19 -0600 } 16, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 16, 27 -- Content-class: urn:content-classes:message } 16, 27 -- MIME-Version: 1.0 } 16, 27 -- Content-Type: text/plain; } 16, 27 -- charset="us-ascii" } 16, 27 -- Subject: RE: [Microscopy] Atomic positions database and software } 16, 27 -- Date: Tue, 28 Nov 2006 07:19:00 -0600 } 16, 27 -- Message-ID: } {62C8A7975D4AE545A23A0CD278BB20A907DFB975-at-DPLEVS1.ad.uop.com} } 16, 27 -- In-Reply-To: {200611280215.kAS2FG1s023788-at-ns.microscopy.com} } 16, 27 -- X-MS-Has-Attach: } 16, 27 -- X-MS-TNEF-Correlator: } 16, 27 -- Thread-Topic: [Microscopy] Atomic positions database and software } 16, 27 -- Thread-Index: AccSkxDEYrvna2kATX2F0pAiPeAQBwAW/BHw } 16, 27 -- From: "Sinkler, Wharton" {Wharton.Sinkler-at-uop.com} } 16, 27 -- To: {jafarhan-at-rci.rutgers.edu} } 16, 27 -- Cc: {microscopy-at-microscopy.com} } 16, 27 -- X-OriginalArrivalTime: 28 Nov 2006 13:18:19.0919 (UTC) } FILETIME=[AC3819F0:01C712EF] } 16, 27 -- Content-Transfer-Encoding: 8bit } 16, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id kASDIKln003069 } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 26 -- From Larry.Thomas-at-pnl.gov Tue Nov 28 10:44:26 2006 6, 26 -- Received: from emailgw01.pnl.gov (emailgw01.pnl.gov [192.101.109.33]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kASGiPgB019046 6, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Nov 2006 10:44:26 -0600 6, 26 -- Received: from pnlmse21.pnl.gov ([130.20.128.55]) 6, 26 -- by emailgw01.pnl.gov with ESMTP; 28 Nov 2006 08:44:25 -0800 6, 26 -- X-IronPort-AV: i="4.09,470,1157353200"; 6, 26 -- d="scan'208"; a="12730052:sNHT31707347" 6, 26 -- Received: from EMAIL02.pnl.gov ([130.20.128.221]) by pnlmse21.pnl.gov with Microsoft SMTPSVC(6.0.3790.1830); 6, 26 -- Tue, 28 Nov 2006 08:44:25 -0800 6, 26 -- Received: from 130.20.26.238 ([130.20.26.238]) by EMAIL02.pnl.gov ([130.20.128.155]) via Exchange Front-End Server webmaili.pnl.gov ([130.20.248.53]) with Microsoft Exchange Server HTTP-DAV ; 6, 26 -- Tue, 28 Nov 2006 16:44:25 +0000 6, 26 -- User-Agent: Microsoft-Entourage/11.2.4.060510 6, 26 -- Date: Tue, 28 Nov 2006 08:44:24 -0800 6, 26 -- Subject: [Microscopy] RE: Atomic positions database and software 6, 26 -- From: "Thomas, Larry (PNNL)" {Larry.Thomas-at-pnl.gov} 6, 26 -- To: {Microscopy-at-microscopy.com} 6, 26 -- Message-ID: {C191A768.16DF%Larry.Thomas-at-pnl.gov} 6, 26 -- Thread-Topic: [Microscopy] RE: Atomic positions database and software 6, 26 -- Thread-Index: AccTDHXJtF8OZH7/EduQ8QAWyzlS+g== 6, 26 -- In-Reply-To: {200611281323.kASDNfSK008566-at-ns.microscopy.com} 6, 26 -- Mime-version: 1.0 6, 26 -- Content-type: text/plain; 6, 26 -- charset="US-ASCII" 6, 26 -- Content-transfer-encoding: 7bit 6, 26 -- X-OriginalArrivalTime: 28 Nov 2006 16:44:25.0222 (UTC) FILETIME=[76839A60:01C7130C] ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (blegge-at-cwisp.ca) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, November 28, 2006 at 12:21:31 ---------------------------------------------------------------------------
Email: blegge-at-cwisp.ca Name: Brian Legge
Education: 9-12th Grade High School
Location: Canada
Question: Hello I am looking for a source for OM sample slides. A school in Cyprus needs to upgrade their science studies facilities and slides are needed.They are using a British based curriculum. Human tissue- nerve, spinal chord,sperm and other mammal tissues etc as well as botany -roots, stems, leaves etc. Where do your school boards obtain slides for grades 9-12? Thank you
Hello All FUNDATEL Foundation, a non profit organization (www.fundatel.org.ar) , is looking for used ( and working ) TEM, SEM and SPM ( and related equipment ) to received it in DONATION. This equipment will be used in our R+D proyects in the biosensors and MEM´s area. Please contact us as soon as posible, we response inmediatly and pay all the costs of shipping and handling.
Best Regards
-- Fernando D. Balducci President FUNDATEL Foundation Parana - Entre Rios Argentina
email: fernando.balducci-at-fundatel.org.ar
==============================Original Headers============================== 7, 23 -- From fundatel-at-gmail.com Tue Nov 28 13:52:54 2006 7, 23 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.233]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kASJqr1o013219 7, 23 -- for {microscopy-at-microscopy.com} ; Tue, 28 Nov 2006 13:52:54 -0600 7, 23 -- Received: by wx-out-0506.google.com with SMTP id h30so2249292wxd 7, 23 -- for {microscopy-at-microscopy.com} ; Tue, 28 Nov 2006 11:52:52 -0800 (PST) 7, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 23 -- s=beta; d=gmail.com; 7, 23 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 7, 23 -- b=KyQO+kjrPiThHX3h73W9mnjo9NvjMyzojiCGBl3jG2HPUkGoX1PfyeYkiDA1MVR1JXW/54Fbs45mOEE5yuhRUHzXAyHlG4Edu8ZPz5ME5wTPmtPkUj3bISTyS9E3cltRzPxJB/QuSlhaEN5fFIuqeH5eVRPO2+GYiHI6xKmbGQg= 7, 23 -- Received: by 10.70.44.4 with SMTP id r4mr2353797wxr.1164743572268; 7, 23 -- Tue, 28 Nov 2006 11:52:52 -0800 (PST) 7, 23 -- Received: by 10.70.62.6 with HTTP; Tue, 28 Nov 2006 11:52:51 -0800 (PST) 7, 23 -- Message-ID: {715613900611281152n234253cma16cfdfcf8827a4a-at-mail.gmail.com} 7, 23 -- Date: Tue, 28 Nov 2006 16:52:51 -0300 7, 23 -- From: Fundatel {fundatel-at-gmail.com} 7, 23 -- To: microscopy-at-microscopy.com 7, 23 -- Subject: looking for SEM and TEM 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 23 -- Content-Disposition: inline 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kASJqr1o013219 ==============================End of - Headers==============================
Has anyone yet tried the newish Microsoft VX-6000 Webcam? It claims to give 5MP for stills, it's cheap, I'm tempted to get one, rip the lens off, and patch it onto the top of a microscope with sticky tape. Not much to lose.
cheers
rtch
O} } Dear Mike, } } Do you want colour or B&W + filters? } } Have you thought of an Astronomical camera? $1500 is tight, but } it } might cover an Artemis camera. I'd recommend one of the better Sony } chips, e.g. ICX285AL or ICX285AQ. } http://www.artemisccd.co.uk/icx285c.htm } http://www.atik-instruments.com/ } } Good luck, } } Austin } } P.S. I'm not financially involved with Artemis; but I do have one of } their excellent cameras. } } } Organization: NDT } } } } Title-Subject: [Filtered] price of used ccd camera } } } } Question: Hi, } } } } we are looking for a pre-owned ccd camera, such as optronics, } } Qimaging, Cooke, Roper, etc that can support our brightfield and/or } } fluorescent digital imagaing and quantitative analysis (volumentric } } analysis and cell, spine counting). Due to our budget limit, we can } } only offer less than USD1500. Please let me know if you have } } available one for sale or know the right party we can contact with, } } thanks! } } } } Mike } } } } -------------------------------------------------------------------- } } ------- } }
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 10, 27 -- From r.sims-at-auckland.ac.nz Tue Nov 28 14:04:05 2006 10, 27 -- Received: from harpo.itss.auckland.ac.nz (mailhost-old.auckland.ac.nz [130.216.190.13]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kASK44C9024006 10, 27 -- for {microscopy-at-microscopy.com} ; Tue, 28 Nov 2006 14:04:04 -0600 10, 27 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 10, 27 -- by harpo.itss.auckland.ac.nz (Postfix) with ESMTP id 5BB0F34E30; 10, 27 -- Wed, 29 Nov 2006 09:04:03 +1300 (NZDT) 10, 27 -- Received: from harpo.itss.auckland.ac.nz ([127.0.0.1]) 10, 27 -- by localhost (smtpc.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 10, 27 -- with ESMTP id 22013-22; Wed, 29 Nov 2006 09:04:03 +1300 (NZDT) 10, 27 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) 10, 27 -- by harpo.itss.auckland.ac.nz (Postfix) with ESMTP id 29B9B34C07; 10, 27 -- Wed, 29 Nov 2006 09:04:03 +1300 (NZDT) 10, 27 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 10, 27 -- To: auntdaisy-at-gmail.com 10, 27 -- Date: Wed, 29 Nov 2006 09:09:03 +1300 10, 27 -- MIME-Version: 1.0 10, 27 -- Subject: Re: [Microscopy] Re: viaWWW: used ccd camera 10, 27 -- Cc: microscopy-at-microscopy.com 10, 27 -- Message-ID: {456D4E2F.20779.D3D19-at-localhost} 10, 27 -- Priority: normal 10, 27 -- In-reply-to: {200611271425.kAREPLLD003633-at-ns.microscopy.com} 10, 27 -- X-mailer: Pegasus Mail for Windows (4.21c) 10, 27 -- Content-type: text/plain; charset=US-ASCII 10, 27 -- Content-transfer-encoding: 7BIT 10, 27 -- Content-description: Mail message body 10, 27 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
Two post-doctoral positions are open in the laboratory of Prof. H. Kumar Wickramasinghe at the University of California, Irvine. The field of research is the Development of Novel Scanning Probe Instruments in Biology. We are seeking skilled individuals with a solid background in Scanning Probe Microscopy, with specific focus on instrument development.
One position requires a Ph.D. in Electrical Engineering, Applied Physics or equivalent with an emphasis in Biology. http://www.eng.uci.edu/node/1103
One position requires a Ph.D. in Biophysics, Bioengineering, Biology or equivalent. http://www.eng.uci.edu/node/1105
Both positions require previous demonstrated accomplishments in scanning probe instrument development.
Application deadline: Open until filled
Please send your curriculum vitae, a list of publications, and names of at least three references to:
Professor H. Kumar Wickramasinghe Department of Electrical Engineering and Computer Science 325 Engineering Tower University of California, Irvine Irvine, CA 92696-2175
Alternatively, materials may be submitted electronically to: Professor H. Kumar Wickramasinghe at: hkwick-at-uci.edu
The University of California, Irvine is an equal opportunity employer committed to excellence through diversity.
==============================Original Headers============================== 13, 21 -- From kerem.unal-at-sbcglobal.net Tue Nov 28 15:57:32 2006 13, 21 -- Received: from smtp109.sbc.mail.mud.yahoo.com (smtp109.sbc.mail.mud.yahoo.com [68.142.198.208]) 13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kASLvTLe005069 13, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Nov 2006 15:57:31 -0600 13, 21 -- Received: (qmail 83042 invoked from network); 28 Nov 2006 21:57:26 -0000 13, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 13, 21 -- s=s1024; d=sbcglobal.net; 13, 21 -- h=Received:X-YMail-OSG:Mime-Version:Content-Transfer-Encoding:Message-Id:Content-Type:To:From:Subject:Date:X-Mailer; 13, 21 -- b=Cxndd+lNLpEhKFihvzOqkpbfNH2eMMlysku+X8Y5xxyoskAaVhls//w44kNGds9zqzo5737XTlwYiVnLQBfMbXEepTCRfMcgSHWtEFvYQLZfQYhlL/Omn67qHI3wOQaHy3xxbNyjQKDlEWUfBAQRbZZHwgllte3nxxAjlYo1Jkc= ; 13, 21 -- Received: from unknown (HELO ?128.200.211.163?) (kerem.unal-at-sbcglobal.net-at-128.200.211.163 with plain) 13, 21 -- by smtp109.sbc.mail.mud.yahoo.com with SMTP; 28 Nov 2006 21:57:26 -0000 13, 21 -- X-YMail-OSG: vkNzBH8VM1lmdspvE9aFCGVhYB7gYgVNBhHf_.o04Rgei1laIVH_sQDutSgOC8E8nWa72NCMraLCSB948Nt44YAwvu2AHGv.9wgWX7cV5sAke5TQjPlsjQ-- 13, 21 -- Mime-Version: 1.0 (Apple Message framework v752.3) 13, 21 -- Content-Transfer-Encoding: 7bit 13, 21 -- Message-Id: {B6027B1E-E269-4709-8588-9E67F496A7F7-at-sbcglobal.net} 13, 21 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 13, 21 -- To: Microscopy-at-microscopy.com 13, 21 -- From: Kerem Uenal {kerem.unal-at-sbcglobal.net} 13, 21 -- Subject: Two postdoctoral scholar positions available 13, 21 -- Date: Tue, 28 Nov 2006 13:57:15 -0800 13, 21 -- X-Mailer: Apple Mail (2.752.3) ==============================End of - Headers==============================
This message only applies to New Zealand laboratories.
Hello fellow New Zealand histologists
We urgently require some Shandon xylene substitute. Our order for new stock has been delayed and we have a researcher who is desperately seeking Shandon (sad title for a new movie maybe?). Can you help PLEASE!?!??
Kind Regards Matthew Downes -- Matthew Downes Electron Microscopy Technician Department of Zoology University of Otago P.O.Box 56 Dunedin New Zealand.
It doesn't say (or I couldn't find) what formats for images are supported. If it is JPG, you have compression artifacts in addition to the interpolation. If you do put that on a microscope, let me know how it works.
Mike
Michael Bode, Ph.D.
General Manager
OLYMPUS SOFT IMAGING SOLUTIONS 12596 West Bayaud Ave #300 Lakewood, CO 80228 USA Tel.: +1 (303) 234-9270 Fax.: +1 (303) 234-9271 E-mail: Mike.Bode-at-olympus-sis.com www.olympus-sis.com
-----Original Message----- X-from: r.sims-at-auckland.ac.nz [mailto:r.sims-at-auckland.ac.nz] Sent: Tuesday, November 28, 2006 13:09 To: Mike Bode
Has anyone yet tried the newish Microsoft VX-6000 Webcam? It claims to give 5MP for stills, it's cheap, I'm tempted to get one, rip the lens off, and patch it onto the top of a microscope with sticky tape. Not much to lose.
cheers
rtch
O} } Dear Mike, } } Do you want colour or B&W + filters? } } Have you thought of an Astronomical camera? $1500 is tight, but } it } might cover an Artemis camera. I'd recommend one of the better Sony } chips, e.g. ICX285AL or ICX285AQ. } http://www.artemisccd.co.uk/icx285c.htm } http://www.atik-instruments.com/ } } Good luck, } } Austin } } P.S. I'm not financially involved with Artemis; but I do have one of } their excellent cameras. } } } Organization: NDT } } } } Title-Subject: [Filtered] price of used ccd camera } } } } Question: Hi, } } } } we are looking for a pre-owned ccd camera, such as optronics, } } Qimaging, Cooke, Roper, etc that can support our brightfield and/or } } fluorescent digital imagaing and quantitative analysis (volumentric } } analysis and cell, spine counting). Due to our budget limit, we can
} } only offer less than USD1500. Please let me know if you have } } available one for sale or know the right party we can contact with, } } thanks! } } } } Mike } } } } -------------------------------------------------------------------- } } ------- } }
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 10, 27 -- From r.sims-at-auckland.ac.nz Tue Nov 28 14:04:05 2006 10, 27 -- Received: from harpo.itss.auckland.ac.nz (mailhost-old.auckland.ac.nz [130.216.190.13]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kASK44C9024006 10, 27 -- for {microscopy-at-microscopy.com} ; Tue, 28 Nov 2006 14:04:04 -0600 10, 27 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 10, 27 -- by harpo.itss.auckland.ac.nz (Postfix) with ESMTP id 5BB0F34E30; 10, 27 -- Wed, 29 Nov 2006 09:04:03 +1300 (NZDT) 10, 27 -- Received: from harpo.itss.auckland.ac.nz ([127.0.0.1]) 10, 27 -- by localhost (smtpc.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 10, 27 -- with ESMTP id 22013-22; Wed, 29 Nov 2006 09:04:03 +1300 (NZDT) 10, 27 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) 10, 27 -- by harpo.itss.auckland.ac.nz (Postfix) with ESMTP id 29B9B34C07; 10, 27 -- Wed, 29 Nov 2006 09:04:03 +1300 (NZDT) 10, 27 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 10, 27 -- To: auntdaisy-at-gmail.com 10, 27 -- Date: Wed, 29 Nov 2006 09:09:03 +1300 10, 27 -- MIME-Version: 1.0 10, 27 -- Subject: Re: [Microscopy] Re: viaWWW: used ccd camera 10, 27 -- Cc: microscopy-at-microscopy.com 10, 27 -- Message-ID: {456D4E2F.20779.D3D19-at-localhost} 10, 27 -- Priority: normal 10, 27 -- In-reply-to: {200611271425.kAREPLLD003633-at-ns.microscopy.com} 10, 27 -- X-mailer: Pegasus Mail for Windows (4.21c) 10, 27 -- Content-type: text/plain; charset=US-ASCII 10, 27 -- Content-transfer-encoding: 7BIT 10, 27 -- Content-description: Mail message body 10, 27 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
==============================Original Headers============================== 25, 24 -- From Mike.Bode-at-olympus-sis.com Tue Nov 28 17:23:41 2006 25, 24 -- Received: from mail.soft-imaging.de (ns.soft-imaging.de [62.180.61.130]) 25, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kASNNeiZ028611 25, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Nov 2006 17:23:41 -0600 25, 24 -- Received: from muenster.soft-imaging.net (muenster.soft-imaging.de [10.26.20.9]) 25, 24 -- by mail.soft-imaging.de (8.13.3/8.13.3/SuSE Linux 0.7) with ESMTP id kASNO7O1019517 25, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Nov 2006 00:24:09 +0100 25, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 25, 24 -- Content-class: urn:content-classes:message 25, 24 -- MIME-Version: 1.0 25, 24 -- Content-Type: text/plain; 25, 24 -- charset="us-ascii" 25, 24 -- Subject: RE: [Microscopy] viaWWW: used ccd camera 25, 24 -- Date: Wed, 29 Nov 2006 00:17:06 +0100 25, 24 -- Message-ID: {78B53BA1C5A2D9449EDA30A98800EC9462733C-at-ms-s-gws.soft-imaging.net} 25, 24 -- In-Reply-To: {200611282008.kASK8pmj032585-at-ns.microscopy.com} 25, 24 -- X-MS-Has-Attach: 25, 24 -- X-MS-TNEF-Correlator: 25, 24 -- Thread-Topic: [Microscopy] viaWWW: used ccd camera 25, 24 -- Thread-Index: AccTKQhK9v1TqI15Qr6b3migYulMYQAGVveg 25, 24 -- From: "Mike Bode" {Mike.Bode-at-olympus-sis.com} 25, 24 -- To: {Microscopy-at-microscopy.com} 25, 24 -- Content-Transfer-Encoding: 8bit 25, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kASNNeiZ028611 ==============================End of - Headers==============================
for $1,500 you might take a look at the Nikon D200 - Street pricing seems to be } $1400 but then you'd also need the opticla coupling if you no not already a 35mm / f-mount setup. The D200 has a very low vibration mirror/shutter system and mirror-up shutter delay on it (10.2megapixel ). Our tests here shows it does well on even light weight scopes (Nikon optiphot), and great job with general fluorescence. However it does not integrate with quantitative 3-D rendering software packages.
On 27 Nov 2006 at 3:32, neurowu-at-yahoo.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both neurowu-at-yahoo.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: neurowu-at-yahoo.com } Name: mike } } Organization: NDT } } Title-Subject: [Filtered] price of used ccd camera } } Question: Hi, } } we are looking for a pre-owned ccd camera, such as optronics, } Qimaging, Cooke, Roper, etc that can support our brightfield and/or } fluorescent digital imagaing and quantitative analysis (volumentric } analysis and cell, spine counting). Due to our budget limit, we can } only offer less than USD1500. Please let me know if you have } available one for sale or know the right party we can contact with, } thanks! } } Mike } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 12 -- From zaluzec-at-microscopy.com Mon Nov 27 03:31:04 2006 } 8, 12 -- Received: from [10.241.76.246] (mk194048137133.a1.net [194.48.137.133]) } 8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAR9V3G0016973 } 8, 12 -- for {microscopy-at-microscopy.com} ; Mon, 27 Nov 2006 03:31:03 -0600 } 8, 12 -- Mime-Version: 1.0 } 8, 12 -- X-Sender: (Unverified) } 8, 12 -- Message-Id: {p06020402c19060606e5d-at-[10.241.76.246]} } 8, 12 -- Date: Mon, 27 Nov 2006 03:29:36 -0600 } 8, 12 -- To: microscopy-at-microscopy.com } 8, 12 -- From: neurowu-at-yahoo.com (by way of MicroscopyListserver) } 8, 12 -- Subject: viaWWW: used ccd camera } 8, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"A main-frame: The biggest PC peripheral available."
==============================Original Headers============================== 9, 32 -- From edelmare-at-muohio.edu Wed Nov 29 14:26:44 2006 9, 32 -- Received: from spamfirewall.muohio.edu (wallaby.mcs.muohio.edu [134.53.6.28]) 9, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kATKQh0l010967 9, 32 -- for {microscopy-at-Microscopy.com} ; Wed, 29 Nov 2006 14:26:44 -0600 9, 32 -- X-ASG-Debug-ID: 1164832003-436c004a0000-Dem1zR 9, 32 -- X-Barracuda-URL: http://spamfirewall.muohio.edu:80/cgi-bin/mark.cgi 9, 32 -- X-Barracuda-Connect: mulnx23.mcs.muohio.edu[134.53.6.10] 9, 32 -- X-Barracuda-Start-Time: 1164832003 9, 32 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 9, 32 -- by spamfirewall.muohio.edu (Spam Firewall) with ESMTP 9, 32 -- id 40A23A0142; Wed, 29 Nov 2006 15:26:43 -0500 (EST) 9, 32 -- Received: from [192.168.1.23] ([134.53.14.105]) 9, 32 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id kATKQh2S014041; 9, 32 -- Wed, 29 Nov 2006 15:26:43 -0500 9, 32 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 9, 32 -- To: neurowu-at-yahoo.com 9, 32 -- Date: Wed, 29 Nov 2006 15:26:42 -0500 9, 32 -- MIME-Version: 1.0 9, 32 -- X-ASG-Orig-Subj: Re: [Microscopy] viaWWW: used ccd camera 9, 32 -- Subject: Re: [Microscopy] viaWWW: used ccd camera 9, 32 -- CC: microscopy-at-Microscopy.com 9, 32 -- Message-ID: {456DA6B2.30327.17AE951-at-edelmare.muohio.edu} 9, 32 -- Priority: normal 9, 32 -- In-reply-to: {200611270932.kAR9WH2H018275-at-ns.microscopy.com} 9, 32 -- References: {200611270932.kAR9WH2H018275-at-ns.microscopy.com} 9, 32 -- X-mailer: Pegasus Mail for Windows (4.41) 9, 32 -- Content-type: text/plain; charset=US-ASCII 9, 32 -- Content-transfer-encoding: 7BIT 9, 32 -- Content-description: Mail message body 9, 32 -- X-Barracuda-Virus-Scanned: by Barracuda Spam Firewall at muohio.edu 9, 32 -- X-Barracuda-Spam-Score: 0.00 9, 32 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=3.5 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=1000.0 ==============================End of - Headers==============================
Greetings Everyone, Recently, one of the three 24 volt lens power supplies on our five year old TF20 died. We found out that 24 volt replacements are no longer available and have not been manufactured for 5 or 6 years. The current replacement is two 38 volt power supplies. Consequently, we have two spare 24 volt units that we will not need. If someone out there would like to have them, please contact me. We just ask that you pay the shipping. Don
Mr. Donald Gantz Research Histologist/Electron Microscopist Dept. Physiology and Biophysics Center for Advanced Biomedical Research Boston University School of Medicine 715 Albany Street Boston, MA 02118 phone: 617-638-4017
==============================Original Headers============================== 3, 24 -- From gantz-at-bu.edu Wed Nov 29 14:39:38 2006 3, 24 -- Received: from relay9.bu.edu (relay9.bu.edu [128.197.27.239]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kATKdcGA022007 3, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 29 Nov 2006 14:39:38 -0600 3, 24 -- X-Envelope-From: gantz-at-bu.edu 3, 24 -- Received: from biophysics1.bumc.bu.edu (biophysics1.bumc.bu.edu [155.41.209.120]) 3, 24 -- by relay9.bu.edu (8.13.8/8.13.8) with ESMTP id kATKdIs4022494 3, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 29 Nov 2006 15:39:18 -0500 3, 24 -- Received: from gantz (dhcp209-127.bumc.bu.edu [155.41.209.127]) 3, 24 -- by biophysics1.bumc.bu.edu (8.13.8/8.12.11) with SMTP id kATKdIFw003810 3, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 29 Nov 2006 15:39:18 -0500 3, 24 -- Message-ID: {005e01c713f6$c4ff3290$7fd1299b-at-gantz} 3, 24 -- From: "Don Gantz" {gantz-at-bu.edu} 3, 24 -- To: {Microscopy-at-MSA.Microscopy.Com} 3, 24 -- Subject: TF20 24volt power supplies 3, 24 -- Date: Wed, 29 Nov 2006 15:41:38 -0500 3, 24 -- MIME-Version: 1.0 3, 24 -- Content-Type: text/plain; 3, 24 -- charset="iso-8859-1" 3, 24 -- Content-Transfer-Encoding: 7bit 3, 24 -- X-Priority: 3 3, 24 -- X-MSMail-Priority: Normal 3, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1807 3, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1807 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both doodyl-at-west-greene.k12.pa.us as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: West Greene High School in SW PA has a pSEM (EDS) operated by and for the students. It was purchased through a grant from the Appalachian Regional Commission and the help and continuing support of the RJ Lee Group, Inc. I am the Chemistry teacher and Curriculum Consultant responsible for planning and implementing curriculum with the SEM. Our current Grant through the ARC is to train other teachers in the tri-county area in using the SEM in the classroom; virtually, remotely, or physically through a Workshop in March-07, offered to the tri-county teachers (Greene, Washington, Fayette). Because this program is relatively new, I am open to ideas, coments, etc. Please feel free to contact me at any time. 724-499-5782 at the West Greene SEM Lab. Lurea Doody
"really old) information"??? Hey now I love the MT-2's! (The MT-2b is my favorite). Still have one in my lab. Did you get an answer to your question? If not I can work one up for you.
But basically at 500nm (0.5um) if you have to be accurate of 500nm - you need to use the section color. The adjustment wheel (On top of microtome) works interactively with the Thickness wheel on left front side (and they are calibrated in angstroms = 0.5um = 500nm = 5000Ĺ). HOWEVER, the zero point of the calibration of the top knob is usually very far off if anyone has ever taken the case cover off the microtome.
On 21 Nov 2006 at 8:30, gwe-at-ufl.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } X-from: "Dr. Michael Horst" {HORST_MN-at-Mercer.edu} } To: "Greg Erdos" {gwe-at-ufl.edu} } Sent: Tuesday, November 21, 2006 9:00 AM } Subject: Help with an MT-2 ultramicrotome } } } } Hi- } } I would like to ask if anyone can help me understand the relationship } } between the adjustment knob and the five horizontal lines scribed into the } } shaft of the pivot arm. My goal is to cut 0.5 micron sections but the } } Instruction Manual does not address the use of these five slashes and how } } they relate to the adustment knob. } } Any one out there remember this (really old) information ? If so, please } } email me at horst_mn-at-mercer.edu } } Thanks! } } } } Mike } } } } Michael N. Horst } } School of Medicine } } Mercer University } } 1550 College St. } } Macon GA 31207 } } } } } } } ==============================Original Headers============================== } 5, 27 -- From gwe-at-ufl.edu Tue Nov 21 08:30:13 2006 } 5, 27 -- Received: from imf20aec.mail.bellsouth.net (imf20aec.mail.bellsouth.net [205.152.59.68]) } 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kALEUDli009598 } 5, 27 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2006 08:30:13 -0600 } 5, 27 -- Received: from ibm64aec.bellsouth.net ([70.152.55.172]) } 5, 27 -- by imf20aec.mail.bellsouth.net with ESMTP } 5, 27 -- id {20061121143012.QISX23001.imf20aec.mail.bellsouth.net-at-ibm64aec.bellsouth.net} } 5, 27 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2006 09:30:12 -0500 } 5, 27 -- Received: from ufcdd0b59c8a4d ([70.152.55.172]) by ibm64aec.bellsouth.net } 5, 27 -- with SMTP } 5, 27 -- id {20061121143012.QLXU16860.ibm64aec.bellsouth.net-at-ufcdd0b59c8a4d} } 5, 27 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2006 09:30:12 -0500 } 5, 27 -- Message-ID: {00b301c70d79$87f0af10$4d399846-at-ufcdd0b59c8a4d} } 5, 27 -- From: "greg erdos" {gwe-at-ufl.edu} } 5, 27 -- To: {microscopy-at-microscopy.com} } 5, 27 -- Subject: Fw: Help with an MT-2 ultramicrotome } 5, 27 -- Date: Tue, 21 Nov 2006 09:30:02 -0500 } 5, 27 -- MIME-Version: 1.0 } 5, 27 -- Content-Type: text/plain; } 5, 27 -- format=flowed; } 5, 27 -- charset="iso-8859-1"; } 5, 27 -- reply-type=original } 5, 27 -- Content-Transfer-Encoding: 7bit } 5, 27 -- X-Priority: 3 } 5, 27 -- X-MSMail-Priority: Normal } 5, 27 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2905 } 5, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"A main-frame: The biggest PC peripheral available."
==============================Original Headers============================== 11, 33 -- From edelmare-at-muohio.edu Thu Nov 30 10:11:16 2006 11, 33 -- Received: from spamfirewall.muohio.edu (wallaby.mcs.muohio.edu [134.53.6.28]) 11, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAUGBG2s006889 11, 33 -- for {microscopy-at-Microscopy.com} ; Thu, 30 Nov 2006 10:11:16 -0600 11, 33 -- X-ASG-Debug-ID: 1164903075-209b01090000-Dem1zR 11, 33 -- X-Barracuda-URL: http://spamfirewall.muohio.edu:80/cgi-bin/mark.cgi 11, 33 -- X-Barracuda-Connect: mulnx24.mcs.muohio.edu[134.53.6.11] 11, 33 -- X-Barracuda-Start-Time: 1164903075 11, 33 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 11, 33 -- by spamfirewall.muohio.edu (Spam Firewall) with ESMTP 11, 33 -- id E5097B7D25; Thu, 30 Nov 2006 11:11:15 -0500 (EST) 11, 33 -- Received: from [192.168.1.23] ([134.53.14.105]) 11, 33 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id kAUGBFDS004845; 11, 33 -- Thu, 30 Nov 2006 11:11:15 -0500 11, 33 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 11, 33 -- To: HORST_MN-at-Mercer.edu 11, 33 -- Date: Thu, 30 Nov 2006 11:11:14 -0500 11, 33 -- MIME-Version: 1.0 11, 33 -- X-ASG-Orig-Subj: Re: [Microscopy] Fw: Help with an MT-2 ultramicrotome 11, 33 -- Subject: Re: [Microscopy] Fw: Help with an MT-2 ultramicrotome 11, 33 -- CC: gwe-at-ufl.edu, microscopy-at-Microscopy.com 11, 33 -- Message-ID: {456EBC52.17818.5B75BF6-at-edelmare.muohio.edu} 11, 33 -- Priority: normal 11, 33 -- In-reply-to: {200611211430.kALEUpgj010883-at-ns.microscopy.com} 11, 33 -- References: {200611211430.kALEUpgj010883-at-ns.microscopy.com} 11, 33 -- X-mailer: Pegasus Mail for Windows (4.41) 11, 33 -- Content-type: text/plain; charset=ISO-8859-1 11, 33 -- Content-description: Mail message body 11, 33 -- X-Barracuda-Virus-Scanned: by Barracuda Spam Firewall at muohio.edu 11, 33 -- X-Barracuda-Spam-Score: 0.00 11, 33 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=3.5 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=1000.0 11, 33 -- Content-Transfer-Encoding: 8bit 11, 33 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id kAUGBG2s006889 ==============================End of - Headers==============================
We have a field emission SEM that has massive contamination which appears as a yellowish residue in the specimen chamber and all the way up the column to the gun.
Mass spectroscopy of the residue gave the following results:
We have a number of users looking at polymers, the most likely suspects among the samples that go into the microscope, but so far there do not seem to be any that would result in the above contamination.
The real mystery is 1) all these samples are sputter coated with at least 3nm Pt before imaging. Considering the mobility of the contamination you would think problems would show up with the sputter coater even though vacuum and plasma energy are less than what the samples experience in the FESEM (most people are using low accelerating voltages of 1-5 keV by the way) 2)The yellowish color of the contamination seems odd, most of the samples are grey or black 3)The evidence at this point indicates a rapid rather than gradual rate of contamination, considering the amount of contamination it would seem an entire sample would have to vaporize before the user's eyes to provide enough material (of course they would have to admit to seeing this)
Has anyone else experienced anything like this (the rather seasoned service engineer has not)?
Many thanks for taking the time to read all of this.
Tom
} Tom Stephens } Assistant Research Scientist } Microscopy and Imaging Center } Texas A&M University } College Station, TX 77843 } office phone:979-845-1165 } email:tstephen-at-mic.tamu.edu
==============================Original Headers============================== 11, 20 -- From tstephen-at-mic.tamu.edu Thu Nov 30 11:48:14 2006 11, 20 -- Received: from sr-5-int.cis.tamu.edu (smtp-relay.tamu.edu [165.91.22.120]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAUHmEQ6019711 11, 20 -- for {Microscopy-at-Microscopy.Com} ; Thu, 30 Nov 2006 11:48:14 -0600 11, 20 -- Received: from localhost (localhost.tamu.edu [127.0.0.1]) 11, 20 -- by sr-5-int.cis.tamu.edu (Postfix) with ESMTP id 39F6C6AEF 11, 20 -- for {Microscopy-at-Microscopy.Com} ; Thu, 30 Nov 2006 11:48:14 -0600 (CST) 11, 20 -- Received: from jntcspc.mic.tamu.edu (cyn6400.tamu.edu [165.91.109.143]) 11, 20 -- by sr-5-int.cis.tamu.edu (Postfix) with ESMTP id AEC37730A 11, 20 -- for {Microscopy-at-Microscopy.Com} ; Thu, 30 Nov 2006 11:48:11 -0600 (CST) 11, 20 -- Message-Id: {5.2.0.9.0.20061129143701.02b26ef8-at-mic.tamu.edu} 11, 20 -- X-Sender: tstephen-at-mic.tamu.edu (Unverified) 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.0.9 11, 20 -- Date: Thu, 30 Nov 2006 11:48:13 -0600 11, 20 -- To: Microscopy-at-Microscopy.Com 11, 20 -- From: Tom Stephens {tstephen-at-mic.tamu.edu} 11, 20 -- Subject: SEM - Mystery contamination of FESEM 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 11, 20 -- X-Virus-Scanned: amavisd-new at tamu.edu ==============================End of - Headers==============================
} } Email: doodyl-at-west-greene.k12.pa.us } Name: Lurea Doody } } Organization: West Greene HS, Waynesburg College } } Title-Subject: [Filtered] SEM in my High School } } Question: West Greene High School in SW PA has a pSEM (EDS) operated } by and for the students. It was purchased through a grant from the } Appalachian Regional Commission and the help and continuing support } of the RJ Lee Group, Inc. I am the Chemistry teacher and Curriculum } Consultant responsible for planning and implementing curriculum with } the SEM. Our current Grant through the ARC is to train other teachers } in the tri-county area in using the SEM in the classroom; virtually, } remotely, or physically through a Workshop in March-07, offered to } the tri-county teachers (Greene, Washington, Fayette). Because this } program is relatively new, I am open to ideas, coments, etc. Please } feel free to contact me at any time. 724-499-5782 at the West Greene } SEM Lab. Lurea Doody } } --------------------------------------------------------------------------- } Laura -
Project MICRO is for middle schools, but I try to track what's happening at the high school level. Here's another SEM in a Pennsylvania high school:
"The 2 teachers who have been primarily in charge are Heather Fogel (hef-at-rlasd.k12.pa.us) and Marcie Walizer (mlw-at-rlasd.k12.pa.us). They both teach biology at Red Lion Area Sr. High School." ___________________ And this is from a contact in Ohio:
"To answer your questions, 1) Yes, the van is still active - it's called Tech Trek [A SEM in a truck] 2) There is an SEM still located at Patterson Co-Op High School but not sure if anyone still uses it. The base [Wright-Patterson AFB] also still runs it's SEMeDS program where once a month, classes are brought in the afternoons from 3-6PM to do SEM.
The contact for Tech Trek is: Sharon Nelson (937) 904-8626 Sharon.Nelson-at-wpafb.af.mil The contact for SEMeDS is: Kim Stultz (937) 674-8623 Kimberly.Stultz-at-wpafb.af.mil " _____________________
"David L. Jones" {dljones-at-bestweb.net} is trying to start a SEM program in New York state.
If you get any other responses to your inquiry, will you please let me know? A comprehensive list would help everyone.
-- Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 8, 18 -- From schooley-at-mcn.org Thu Nov 30 12:46:00 2006 8, 18 -- Received: from dns4.mcn.org (dns4.mcn.org [216.150.240.31]) 8, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAUIk05T031504 8, 18 -- for {microscopy-at-microscopy.com} ; Thu, 30 Nov 2006 12:46:00 -0600 8, 18 -- Received: from [66.52.139.70] (helo=[10.0.1.2]) 8, 18 -- by dns4.mcn.org with esmtpa (Exim 4.60) 8, 18 -- (envelope-from {schooley-at-mcn.org} ) 8, 18 -- id J9K44M-0003M3-3D; Thu, 30 Nov 2006 10:45:59 -0800 8, 18 -- Mime-Version: 1.0 8, 18 -- Message-Id: {a06200704c194cc3c076a-at-[10.0.1.2]} 8, 18 -- In-Reply-To: {200611300845.kAU8jUV2024078-at-ns.microscopy.com} 8, 18 -- References: {200611300845.kAU8jUV2024078-at-ns.microscopy.com} 8, 18 -- Date: Thu, 30 Nov 2006 10:27:06 -0800 8, 18 -- To: doodyl-at-west-greene.k12.pa.us 8, 18 -- From: Caroline Schooley {schooley-at-mcn.org} 8, 18 -- Subject: Re: [Microscopy] viaWWW: SEM in my High School 8, 18 -- Cc: microscopy-at-microscopy.com, tpepper-at-iastate.edu 8, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
And I'm sure there must be numerous other commercial sources. (By the way, I have no financial interest in either firm, but have made satisfactory small purchases from both.)
-------Roy Arrowood
==============================Original Headers============================== 7, 20 -- From arrowood-at-utep.edu Thu Nov 30 15:31:47 2006 7, 20 -- Received: from itdsrvmail00.utep.edu (itdsrvmail00.utep.edu [129.108.0.100]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kAULVl4C013220 7, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Nov 2006 15:31:47 -0600 7, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 20 -- Content-class: urn:content-classes:message 7, 20 -- MIME-Version: 1.0 7, 20 -- Content-Type: text/plain; 7, 20 -- charset="us-ascii" 7, 20 -- Subject: in reply to "source for OM sample slides" 7, 20 -- Date: Thu, 30 Nov 2006 14:31:46 -0700 7, 20 -- Message-ID: {8B48F5AF730998488AF7BC9D7D22172001D50946-at-itdsrvmail00.utep.edu} 7, 20 -- X-MS-Has-Attach: 7, 20 -- X-MS-TNEF-Correlator: 7, 20 -- Thread-Topic: in reply to "source for OM sample slides" 7, 20 -- Thread-Index: AccUxvAlZ80lyzGWTEe6c0IXXnyV+g== 7, 20 -- From: "Arrowood, Roy" {arrowood-at-utep.edu} 7, 20 -- To: {Microscopy-at-microscopy.com} 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kAULVl4C013220 ==============================End of - Headers==============================
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