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Email: yxu-at-thinfilm.com Name: yang xu
Organization: Applied ThinFilm Products
Title-Subject: [Filtered] Help to obtain Manuals for Cambridge S-600 SEM
Question: Please help us to obtain the User Manual and the Maintenance Manual for Cambridge S-600 SEM. Your kind help are greatly appreciated.
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Email: phankus-at-yahoo.com Name: pankaj
Organization: UGC INDORE INDIA
Title-Subject: [Filtered] ELECTRON DIFFRACTION SIMULATION SOFTWARE
Question: I AM LOOKING FOR A FREE ELECTRON DIFFRACTION SIMULATION PROGRAM/SOFTWARE (WINDOWS BASED) PLEASE LET ME KNOW FROM WHERE TO GET THE SAME. WITH REGARDS PANKAJ
On Dec 1, 2006, at 3:52 AM, phankus-at-yahoo.com wrote:
} Organization: UGC INDORE INDIA } } Title-Subject: [Filtered] ELECTRON DIFFRACTION SIMULATION SOFTWARE } } Question: I AM LOOKING FOR A FREE ELECTRON DIFFRACTION SIMULATION } PROGRAM/SOFTWARE (WINDOWS BASED) } PLEASE LET ME KNOW FROM WHERE TO GET THE SAME. } WITH REGARDS } PANKAJ } Dear Pankaj, The following was posted on the list on 25 Feb, 2003:
} I favor the plane-wave multislice implementation by Earl Kirkland. In } the frozen phonon approximation, it is arguably the most accurate } algorithm (see D. A. Muller et al Ultramicroscopy 86, 371 (2001)). } Best of all, you get the entire source code and executables for Mac } and Windows for the price of Kirkland's book! The book is "Advanced } Computing in Electron Microscopy", by Earl J. Kirkland, Plenum 1998, } ISBN 0-306-45936-1. It contains a concise summary of electron } scattering and image formation, an extensive treatment of the } plane-wave multislice image simulation method, and advice for doing } accurate simulations with examples. } } This package has one drawback for some users: the user-interface is } command-line only. There is no slick GUI and no graphical help } constructing atomic models. } } } } Good luck! } Paul Voyles } } Assistant Professor } Materials Science and Engineering Department } University of Wisconsin - Madison } 1509 University Ave. } Madison, WI 53706-1595 } Voice: (608) 265-6740 } Fax: (608) 262-8353 } voyles-at-engr.wisc.edu } www.engr.wisc.edu/mse/faculty/voyles_paul.html } It's not quite free, but the book may be worth it to you. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 5, 22 -- From tivol-at-caltech.edu Fri Dec 1 12:15:17 2006 5, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB1IFHkZ011398 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 1 Dec 2006 12:15:17 -0600 5, 22 -- Received: from fire-dog.caltech.edu (fire-dog [192.168.1.4]) 5, 22 -- by water-ox-postvirus (Postfix) with ESMTP 5, 22 -- id 83F3C2EFB5; Fri, 1 Dec 2006 10:15:12 -0800 (PST) 5, 22 -- Received: from [192.168.159.233] (jpix-01.caltech.edu [131.215.2.133]) 5, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP 5, 22 -- id E928D2EF14; Fri, 1 Dec 2006 10:15:07 -0800 (PST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v624) 5, 22 -- In-Reply-To: {200612011152.kB1BqvFN022849-at-ns.microscopy.com} 5, 22 -- References: {200612011152.kB1BqvFN022849-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 5, 22 -- Message-Id: {945f7a0f92105c08c9f0518f97fdbbf6-at-caltech.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 22 -- Subject: Re: [Microscopy] viaWWW: ELECTRON DIFFRACTION SIMULATION SOFTWARE 5, 22 -- Date: Fri, 1 Dec 2006 10:21:09 -0800 5, 22 -- To: microscopy-at-msa.microscopy.com, phankus-at-yahoo.com 5, 22 -- X-Mailer: Apple Mail (2.624) 5, 22 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.3.3 ==============================End of - Headers==============================
We have a Gatan Model 655 Dry Pumping Station (www.gatan.com/holders/655_drypumpingstation.html) equipped with pods for storage of TEM specimens. In particular, TEM cross-sections of integrated circuits using copper interconnects seem to be particularly vulnerable to corrosion in atmosphere. This vacuum station has been essential in protecting the specimens that we put so much work into making, and has been very successful for quite some time. The dry vacuum system consists of a 'drag' pump, which is essentially a turbo pump, backed by a diaphragm type rough pump. Last summer, one of the rubber diaphragms failed in the backing pump, and the system ran for a few days with degraded vacuum. After the diaphragms were replaced and the high vacuum restored to the E-5 Torr range, we found that TEM cross-sections of integrated circuits using copper interconnects were destroyed in just a few hours if stored in this vacuum system. EDX of the copper corrosion product showed the presence of sulfur. We have tried cleaning the storage pods, and part of the inside of the vacuum chamber, but have been totally frustrated in being able to restore the essential function of this vacuum storage system. Any suggestions as to what may have happened, and what we can do to clean up the system would be very much appreciated. Thank you.
John Mardinly Intel Corporation
Disclaimer: This is a communication from this author and does not represent an opinion of Intel Corporation.
==============================Original Headers============================== 6, 30 -- From john.mardinly-at-intel.com Fri Dec 1 13:35:49 2006 6, 30 -- Received: from mga01.intel.com (mga01.intel.com [192.55.52.88]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB1JZnHb024448 6, 30 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 1 Dec 2006 13:35:49 -0600 6, 30 -- Received: from fmsmga001.fm.intel.com ([10.253.24.23]) 6, 30 -- by mga01.intel.com with ESMTP; 01 Dec 2006 11:35:32 -0800 6, 30 -- Received: from scsmsx332.sc.intel.com (HELO scsmsx332.amr.corp.intel.com) ([10.3.90.6]) 6, 30 -- by fmsmga001.fm.intel.com with ESMTP; 01 Dec 2006 11:35:32 -0800 6, 30 -- X-ExtLoop1: 1 6, 30 -- X-IronPort-AV: i="4.09,486,1157353200"; 6, 30 -- d="scan'208"; a="171762165:sNHT19309206" 6, 30 -- Received: from scsmsx413.amr.corp.intel.com ([10.3.90.32]) by scsmsx332.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 6, 30 -- Fri, 1 Dec 2006 11:35:31 -0800 6, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 30 -- Content-class: urn:content-classes:message 6, 30 -- MIME-Version: 1.0 6, 30 -- Content-Type: text/plain; 6, 30 -- charset="us-ascii" 6, 30 -- Subject: Contamination of Vacuum Specimen Storage System 6, 30 -- Date: Fri, 1 Dec 2006 11:35:31 -0800 6, 30 -- Message-ID: {1DF4C4D62339DB4C9C98DF04213995B601F0A84F-at-scsmsx413.amr.corp.intel.com} 6, 30 -- X-MS-Has-Attach: 6, 30 -- X-MS-TNEF-Correlator: 6, 30 -- Thread-Topic: Contamination of Vacuum Specimen Storage System 6, 30 -- Thread-Index: AccVcgLpZR8yy6qqRpi55yhxXPXijQAAIjDgAAAaArAAAVCSIAAB3rtQ 6, 30 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 6, 30 -- To: {Microscopy-at-msa.microscopy.com} 6, 30 -- X-OriginalArrivalTime: 01 Dec 2006 19:35:31.0926 (UTC) FILETIME=[DD2F8F60:01C7157F] 6, 30 -- Content-Transfer-Encoding: 8bit 6, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kB1JZnHb024448 ==============================End of - Headers==============================
I have a chemist who wants to look at nanoparticle distribution in fully hydrated acrylamide gel. Unfortunately the particles are too small to image using cryoFESEM. He wanted to just dump the stuff on a grid and put it in the TEM. After my emphatic "NO", I finally persuaded him that it would have to be dehydrated, embedded and thin sectioned. Of course there will be the inevitable collapse of the gel when dehydrated.
Does anyone have suggestions as to ways to fix the gel to try to stabilize it even a little bit prior to dehydration?
Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 6, 21 -- From dsherman-at-purdue.edu Fri Dec 1 13:44:05 2006 6, 21 -- Received: from 1061exfe04.adpc.purdue.edu (1061exfe04.adpc.purdue.edu [128.210.63.227]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB1Ji5Jr002829 6, 21 -- for {microscopy-at-microscopy.com} ; Fri, 1 Dec 2006 13:44:05 -0600 6, 21 -- Received: from exchange.purdue.edu ([172.21.6.23]) by 1061exfe04.adpc.purdue.edu with Microsoft SMTPSVC(6.0.3790.211); 6, 21 -- Fri, 1 Dec 2006 14:44:05 -0500 6, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 6, 21 -- Fri, 1 Dec 2006 19:44:05 +0000 6, 21 -- User-Agent: Microsoft-Entourage/11.2.5.060620 6, 21 -- Date: Fri, 01 Dec 2006 14:44:04 -0500 6, 21 -- Subject: Embedding acrylamide gel 6, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 6, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 21 -- Message-ID: {C195F034.16EDA%dsherman-at-purdue.edu} 6, 21 -- Thread-Topic: Embedding acrylamide gel 6, 21 -- Thread-Index: AccVgQ5nTOjl94F0EduIegARJN08Mg== 6, 21 -- Mime-version: 1.0 6, 21 -- Content-type: text/plain; 6, 21 -- charset="US-ASCII" 6, 21 -- Content-transfer-encoding: 7bit 6, 21 -- X-OriginalArrivalTime: 01 Dec 2006 19:44:05.0395 (UTC) FILETIME=[0F3CB630:01C71581] ==============================End of - Headers==============================
Is contamination dry or rather oily? Does it look like corrosion (exhibits any adhesion to the surfaces?) Does it prefer to precipitate on any particular materials or at any particular locations?
What vacuum pumps are in the system? Oil diffusion? Oil sealed rotary? Turbo? If fluids are present, what fluids?
Was anything done recently with water cooling system? Did cooling accidents happen? If ODP is present, is ODP cooling line connected in series with electronics cooling line, or in parallel?
If SEM has pneumatic valves, do you check condition of air supply regularly? If water condenses into pneumatic lines, valves will not necessarily fail at once, but they will slow down - that almost certainly will cause vac. fluids surges into high vacuum areas, while ODP fluid itself could be damaged to various degrees.
Vitaly Feingold SIA 2773 Heath Line Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com ----- Original Message ----- X-from: {tstephen-at-mic.tamu.edu} To: {vitalylazar-at-att.net} Sent: Thursday, November 30, 2006 12:50 PM
Laboratory Technician, Microscopy & Imaging Facility, American Museum Of Natural History.
The Laboratory Technician will be responsible for assisting and/or training scientific staff with Scanning Electron Microscopy, X-ray microanalysis, Laser Scanning Confocal Microscopy, Cathodoluminescence Spectroscopy, specimen preparation and image processing and printing; maintain functionality and capability of lab systems to suit the needs of the scientific staff and emerging technologies; interact with curatorial staff and students on diverse projects using microscopy and digital imaging in the fields of zoology, paleontology, anthropology, archeology, and earth and planetary sciences. The ideal candidate will possess knowledge of Scanning Electron Beam instruments and microanalysis and computer hardware/software; display patience, willingness to learn, flexibility, excellent organizational and intra-personal skills. At least one year of full-time Scanning Electron Microscopy experience; BS required; major in biology preferred.
Full time, 35 hrs/week, excellent benefits. Salary: $34,000/year. Please send resumé and cover letter along with 2 letters of recommendation to hrdesk-at-amnh.org All other inquiries should be sent to hrdesk-at-amnh.org The American Museum of Natural History is an Equal Opportunity Employer.
==============================Original Headers============================== 4, 24 -- From mey-at-amnh.org Fri Dec 1 16:06:20 2006 4, 24 -- Received: from lepore.amnh.org (lepore.amnh.org [216.73.241.12]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB1M6Kvj028039 4, 24 -- for {Microscopy-at-microscopy.org} ; Fri, 1 Dec 2006 16:06:20 -0600 4, 24 -- Received: from localhost (localhost [127.0.0.1]) 4, 24 -- by lepore.amnh.org (Postfix) with ESMTP id CBE6B5B768 4, 24 -- for {Microscopy-at-microscopy.org} ; Fri, 1 Dec 2006 17:06:19 -0500 (EST) 4, 24 -- X-Virus-Scanned: amavisd-new at amnh.org 4, 24 -- Received: from lepore.amnh.org ([127.0.0.1]) 4, 24 -- by localhost (lepore.amnh.org [127.0.0.1]) (amavisd-new, port 10024) 4, 24 -- with ESMTP id 631RpXF5I2vq for {Microscopy-at-microscopy.org} ; 4, 24 -- Fri, 1 Dec 2006 17:06:18 -0500 (EST) 4, 24 -- Received: from [172.16.60.30] (216-73-249-246.dynamic.amnh.org [216.73.249.246]) 4, 24 -- by lepore.amnh.org (Postfix) with ESMTP id CEFDA5B765 4, 24 -- for {Microscopy-at-microscopy.org} ; Fri, 1 Dec 2006 17:06:17 -0500 (EST) 4, 24 -- Message-ID: {4570A759.5030709-at-amnh.org} 4, 24 -- Date: Fri, 01 Dec 2006 17:06:17 -0500 4, 24 -- From: Jacob Louis Mey {mey-at-amnh.org} 4, 24 -- User-Agent: Thunderbird 1.5.0.8 (Windows/20061025) 4, 24 -- MIME-Version: 1.0 4, 24 -- To: Microscopy-at-microscopy.org 4, 24 -- Subject: Job Posting: Lab Technician, American Museum of Natural History 4, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 24 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Including tannic acid in the primary fix, following with UrAc or OsO4, has a good reputation for enhancing resistance of lattices against shrinkage. We have used the TA-UrAc sequence in acetone for freeze-substitution of muscle fibers, slam frozen while supported on a thin underlying block of 2.5% agar gel. (Nylon spacers limited squashing during the impact). The agar gel mesh appeared quite reasonably preserved when sections were oriented to include it; I can't say we took any pictures of it, because our interest was in the muscle.
-mike reedy-
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
John, I think that the sulfur came from the rubber diaphragms. I'm surprised, because I thought that they would have been VitonR. Either that or lubrication oil from moving parts that came through the failed diaphragm and was exposed to the system. Was the diaphragm torn or had holes in it? Sulfur's atomic weight just might let it get through a turbo pump. Anyway, I think what you might be able to do is "getter" the sulfur in your system. Get some copper powder and put it in your system for a while. The smaller the powder particles and the more open the container, the better gettering that you will have. Silver ought to work also. It will take some time to getter the sulfur in your system out, but given some time, it should be able to do it. You will also be able to monitor the progress on the copper particles if you periodically change the copper.
In the mean time, you might want to consider storing your samples with our SampleSaverT system. We have a specially vented TEM storage box for the SampleSaverT container system that will take TEM grids. If the samples are FIB cuts, you can store them in the SampleSaverT container in our FortressT FIB holders that will also fit into the SampleSaverT container. The SampleSaverT containers worked for my cross section samples of Low-E glass where there are two layers of Ag exposed. Unfortunately, I haven't gotten any feedback yet from how well they work for copper metallization samples. I expect them to work as well there, but as I said, I don't have hard evidence, yet.
BTW, Generally, the suggested maintenance replacement time on diaphragms for pumps is about a year.
Disclaimer: South Bay Technology, Inc. makes and sells the SampleSaverT container and FortressT FIB holders.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com] Sent: Friday, December 01, 2006 11:40 AM To: Walck-at-SouthBayTech.com
We have a Gatan Model 655 Dry Pumping Station (www.gatan.com/holders/655_drypumpingstation.html) equipped with pods for storage of TEM specimens. In particular, TEM cross-sections of integrated circuits using copper interconnects seem to be particularly vulnerable to corrosion in atmosphere. This vacuum station has been essential in protecting the specimens that we put so much work into making, and has been very successful for quite some time. The dry vacuum system consists of a 'drag' pump, which is essentially a turbo pump, backed by a diaphragm type rough pump. Last summer, one of the rubber diaphragms failed in the backing pump, and the system ran for a few days with degraded vacuum. After the diaphragms were replaced and the high vacuum restored to the E-5 Torr range, we found that TEM cross-sections of integrated circuits using copper interconnects were destroyed in just a few hours if stored in this vacuum system. EDX of the copper corrosion product showed the presence of sulfur. We have tried cleaning the storage pods, and part of the inside of the vacuum chamber, but have been totally frustrated in being able to restore the essential function of this vacuum storage system. Any suggestions as to what may have happened, and what we can do to clean up the system would be very much appreciated. Thank you.
John Mardinly Intel Corporation
Disclaimer: This is a communication from this author and does not represent an opinion of Intel Corporation.
==============================Original Headers============================== 6, 30 -- From john.mardinly-at-intel.com Fri Dec 1 13:35:49 2006 6, 30 -- Received: from mga01.intel.com (mga01.intel.com [192.55.52.88]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB1JZnHb024448 6, 30 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 1 Dec 2006 13:35:49 -0600 6, 30 -- Received: from fmsmga001.fm.intel.com ([10.253.24.23]) 6, 30 -- by mga01.intel.com with ESMTP; 01 Dec 2006 11:35:32 -0800 6, 30 -- Received: from scsmsx332.sc.intel.com (HELO scsmsx332.amr.corp.intel.com) ([10.3.90.6]) 6, 30 -- by fmsmga001.fm.intel.com with ESMTP; 01 Dec 2006 11:35:32 -0800 6, 30 -- X-ExtLoop1: 1 6, 30 -- X-IronPort-AV: i="4.09,486,1157353200"; 6, 30 -- d="scan'208"; a="171762165:sNHT19309206" 6, 30 -- Received: from scsmsx413.amr.corp.intel.com ([10.3.90.32]) by scsmsx332.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 6, 30 -- Fri, 1 Dec 2006 11:35:31 -0800 6, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 30 -- Content-class: urn:content-classes:message 6, 30 -- MIME-Version: 1.0 6, 30 -- Content-Type: text/plain; 6, 30 -- charset="us-ascii" 6, 30 -- Subject: Contamination of Vacuum Specimen Storage System 6, 30 --
Dear Dr. Mardinly, I would suspect that the rubber in the seal that failed is the source of the sulphur and this will react with the copper strongly. Unfortunately, this will be distributed throughout the system by the oil back-streamed by the system failure and can only be removed by very thorough cleaning of all chambers and lines. You might try baking the system after cleaning with solvents to get rig of all the oil. Change the oil in the backing pump and run everything for a week to clean it up. The other thing I have used successfully to clean up an SEM that was contaminating badly was to bleed in a stream of nitrogen gas through a feed through I made with a bit of fine stainless steel tubing and a metering valve. The gas valve was turned on until the SEM baffle valve closed and a bit more. Leave for about one hour. Take a blank port cover, drill a small hole through, glue the SS tubing through the hole, swage the fine metering valve on to the SS tubing. Attach the other side of the metering valve to a bag you can fill with nitrogen gas. A bag fill will last more than an hour at the right gas flow rate.) You can use a freshly-polished piece of copper to test your system. Good luck, Mary Mager Electron Microscopist Materials Eng. UBC #419 - 6350 Stores Rd. Vancouver, B.C. V6T 1Z4 CANADA Phone: 604-822-5648 Fax: 604-822-3619 email: mager-at-interchange.ubc.ca
-----Original Message----- X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com] Sent: Friday, December 01, 2006 11:42 AM To: mager-at-interchange.ubc.ca
We have a Gatan Model 655 Dry Pumping Station (www.gatan.com/holders/655_drypumpingstation.html) equipped with pods for storage of TEM specimens. In particular, TEM cross-sections of integrated circuits using copper interconnects seem to be particularly vulnerable to corrosion in atmosphere. This vacuum station has been essential in protecting the specimens that we put so much work into making, and has been very successful for quite some time. The dry vacuum system consists of a 'drag' pump, which is essentially a turbo pump, backed by a diaphragm type rough pump. Last summer, one of the rubber diaphragms failed in the backing pump, and the system ran for a few days with degraded vacuum. After the diaphragms were replaced and the high vacuum restored to the E-5 Torr range, we found that TEM cross-sections of integrated circuits using copper interconnects were destroyed in just a few hours if stored in this vacuum system. EDX of the copper corrosion product showed the presence of sulfur. We have tried cleaning the storage pods, and part of the inside of the vacuum chamber, but have been totally frustrated in being able to restore the essential function of this vacuum storage system. Any suggestions as to what may have happened, and what we can do to clean up the system would be very much appreciated. Thank you.
John Mardinly Intel Corporation
Disclaimer: This is a communication from this author and does not represent an opinion of Intel Corporation.
==============================Original Headers============================== 6, 30 -- From john.mardinly-at-intel.com Fri Dec 1 13:35:49 2006 6, 30 -- Received: from mga01.intel.com (mga01.intel.com [192.55.52.88]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB1JZnHb024448 6, 30 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 1 Dec 2006 13:35:49 -0600 6, 30 -- Received: from fmsmga001.fm.intel.com ([10.253.24.23]) 6, 30 -- by mga01.intel.com with ESMTP; 01 Dec 2006 11:35:32 -0800 6, 30 -- Received: from scsmsx332.sc.intel.com (HELO scsmsx332.amr.corp.intel.com) ([10.3.90.6]) 6, 30 -- by fmsmga001.fm.intel.com with ESMTP; 01 Dec 2006 11:35:32 -0800 6, 30 -- X-ExtLoop1: 1 6, 30 -- X-IronPort-AV: i="4.09,486,1157353200"; 6, 30 -- d="scan'208"; a="171762165:sNHT19309206" 6, 30 -- Received: from scsmsx413.amr.corp.intel.com ([10.3.90.32]) by scsmsx332.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 6, 30 -- Fri, 1 Dec 2006 11:35:31 -0800 6, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 30 -- Content-class: urn:content-classes:message 6, 30 -- MIME-Version: 1.0 6, 30 -- Content-Type: text/plain; 6, 30 -- charset="us-ascii" 6, 30 -- Subject: Contamination of Vacuum Specimen Storage System 6, 30 --
On Dec 1, 2006, at 11:44 AM, dsherman-at-purdue.edu wrote:
} Does anyone have suggestions as to ways to fix the gel to try to } stabilize } it even a little bit prior to dehydration? } Dear Debby, You could try high-pressure freezing followed by either freeze substitution or cryosectioning. For that matter, if the gel doesn't form ice crystals, just freezing it then cryosectioning or freeze-sub could work. If you could soak the gel in a cryoprotectant without disturbing the nanoparticles, then there should be no trouble with the freezing, but I don't know how the presence of the cryoprotectant would affect subsequent steps. Good luck. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Fri Dec 1 17:50:06 2006 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB1No5GQ007704 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 1 Dec 2006 17:50:05 -0600 4, 22 -- Received: from water-dog.caltech.edu (water-dog [192.168.1.26]) 4, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id 4C59735818 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 1 Dec 2006 15:50:05 -0800 (PST) 4, 22 -- Received: from [192.168.159.233] (jpix-01.caltech.edu [131.215.2.133]) 4, 22 -- by wood-ox.its.caltech.edu (Postfix) with ESMTP id D588E2EEE2 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 1 Dec 2006 15:50:03 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v624) 4, 22 -- In-Reply-To: {200612011944.kB1JiAeL002991-at-ns.microscopy.com} 4, 22 -- References: {200612011944.kB1JiAeL002991-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {778b3010dd77a689e15cffb02c3535d5-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] Embedding acrylamide gel 4, 22 -- Date: Fri, 1 Dec 2006 15:56:05 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.624) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.3.3 ==============================End of - Headers==============================
We had tried covering the nanowires with G1 epoxy and it works fine for us. After the glue cures, grind and flatten the surface, removing the excess glue until the glue is around 0.1mm or less thick. Then prepare the cross-sectional sample as it were a thin film sample. You can glue it to a piece of silicon wafer, slice and disk, then polish it with dimpling machine. During ion milling, you want to use sample oscillation, with glue line perpendicular to the beam. The ion beam should be only allowed to bombard the sample from the backside of the substrate.
Another method is FIB (Focused Ion Beam). We haven't used this method, but it should be practical if you have access to FIB.
Chengyu Song National Center For Electron Microscopy Lawrence Berkeley National Laboratory Berkeley, CA 94720
catherine.bougerol-at-cea.fr wrote:
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==============================Original Headers============================== 7, 25 -- From csong-at-lbl.gov Fri Dec 1 18:40:43 2006 7, 25 -- Received: from mta2.lbl.gov (mta2.lbl.gov [128.3.41.12]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB20egSr019120 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 1 Dec 2006 18:40:42 -0600 7, 25 -- Received: from mta2.lbl.gov (localhost [127.0.0.1]) 7, 25 -- by mta2.lbl.gov (8.13.8/8.13.8) with ESMTP id kB20eeie012025 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 1 Dec 2006 16:40:41 -0800 (PST) 7, 25 -- Received: from lbl.gov (apple-0-5-2-e0-6a-f3.dhcp.lbl.gov [131.243.3.239]) 7, 25 -- by mta2.lbl.gov (8.13.8/8.13.8) with ESMTP id kB20edGN012021; 7, 25 -- Fri, 1 Dec 2006 16:40:39 -0800 (PST) 7, 25 -- Message-ID: {4570BD81.E85379F7-at-lbl.gov} 7, 25 -- Date: Fri, 01 Dec 2006 16:41:39 -0700 7, 25 -- From: chengyu song {csong-at-lbl.gov} 7, 25 -- Reply-To: csong-at-lbl.gov 7, 25 -- Organization: lbl 7, 25 -- X-Mailer: Mozilla 4.73C-CCK-MCD LBNL V4.73 Build 2 (Macintosh; U; PPC) 7, 25 -- X-Accept-Language: en,pdf 7, 25 -- MIME-Version: 1.0 7, 25 -- To: catherine.bougerol-at-cea.fr, microscopy-at-microscopy.com 7, 25 -- Subject: Re: [Microscopy] viaWWW: preparation of nanowires for TEM 7, 25 -- References: {200611281443.kASEhcEe032277-at-ns.microscopy.com} 7, 25 -- Content-Type: text/plain; charset=gb2312; x-mac-type="54455854"; x-mac-creator="4D4F5353" 7, 25 -- Content-Transfer-Encoding: 7bit 7, 25 -- X-Virus-Scanned: ClamAV 0.88.6/2269/Fri Dec 1 10:17:05 2006 on mta2 7, 25 -- X-Virus-Status: Clean ==============================End of - Headers==============================
In the UK 'school boards' offer nothing in the way of actual physical assistence with teaching - I suppose they may recommend specific slide sets if asked though. UK schools and teachers use their own budget and time to prepare lesson aids and buy science equipment. All the UK schools I have taught in had years-old boxes of prepared slides lying about (they keep for many years if prepared properly and not dropped or crushed under an objective by enthusiastic pupils) - so we never had to buy any new specimens. Lots of prepared slides are bit of snooze for under 16s though - a text book/internet search does it quicker and cheaper. Also try growing crystals on the slides as well.
Our lab technicians dealt with all the ordering anyway, we just had to make a general request for the item. You can also buy on ebay but the that is probably more for collectors of science equipment and quality may vary. However in the UK there is a lot of specialised suppliers of scientific equipment for secondary schools, who supply prepared slides. A small selection is:
I'm sure there's something linked in http://www.microscopy-uk.org.uk/
Plus for general microscope interest:
http://www.ukge.co.uk/UK/about.asp (for geological microscopes etc) http://science.nhmccd.edu/biol/slides.html a few pictures of slides + links http://micro.magnet.fsu.edu/ all you need to know about microscopes http://www.101science.com/Microscope.htm http://www.btinternet.com/~stephen.durr/ http://www.mccroneatlas.com http://www.diatoms.co.uk (just fun slides of diatoms and butteryfly scales)
I imagine there are school suppliers nearer to Cyprus, e.g. Greece or Turkey, but postage to Europe from the UK is cheap.
regards
Keith
--------------------------------------------
Dr Keith J Morris Manager Imaging Facilties Cell Biology Division The institute of Ophthalmology 11-43 Bath Street London EC1V 9EL
----- Original Message ----- X-from: {blegge-at-cwisp.ca} To: {keith.morris-at-ucl.ac.uk} Sent: Tuesday, November 28, 2006 7:08 PM
Debbie, Bill, et al
Been a couple of years since I've run an acrylamide gel, either for protein or sequencing. We fixed the gels in 30% Isopropanol/10%Acetic Acid prior to staining with coomassie blue.Destaining involves Acetone and Methanol, but i do not remember the precise information on the destaining solutions. After staining we destained with 16.5%Methanol/7.5% Acetic Acid. For sequencing I used 7% Methanol/5% Acetic acid. My memory is that some people used a formaldehyde fixation for sequencing gels, but I never tried that so do not know how well it would work.
Regards cryofixation/sectioning, you could do that, but it might be like using a 100megaton thermonuclear device to remove a tree stump. Remember that acrylamide is a plastic. Standard dehydration and embedding would work quite well as long as the target beads are held in place. Caveat, have never done it, so really do not know whether Bill or I am right. Maybe you do need the bomb.
This does not fit in our normal formaldehyde/glutaraldehyde fixation but it is consistent with fixation done for IF microscopy
==============================Original Headers============================== 4, 21 -- From paul_hazelton-at-umanitoba.ca Fri Dec 1 19:17:31 2006 4, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB21HV28008406 4, 21 -- for {microscopy-at-microscopy.com} ; Fri, 1 Dec 2006 19:17:31 -0600 4, 21 -- Received: from [130.179.152.89] (cvx-026.cc.umanitoba.ca [130.179.152.89]) 4, 21 -- (authenticated bits=0) 4, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id kB21HQeb006824; 4, 21 -- Fri, 1 Dec 2006 19:17:27 -0600 (CST) 4, 21 -- Message-ID: {4570D421.50609-at-umanitoba.ca} 4, 21 -- Date: Fri, 01 Dec 2006 19:17:21 -0600 4, 21 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca} 4, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 4, 21 -- X-Accept-Language: en-us, en 4, 21 -- MIME-Version: 1.0 4, 21 -- To: dsherman-at-purdue.edu, Microscopy Listserver {microscopy-at-microscopy.com} , 4, 21 -- Bill Tivol {tivol-at-caltech.edu} 4, 21 -- Subject: Re: [Microscopy] Embedding acrylamide gel 4, 21 -- References: {200612011945.kB1Jjmg0006334-at-ns.microscopy.com} 4, 21 -- In-Reply-To: {200612011945.kB1Jjmg0006334-at-ns.microscopy.com} 4, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
There are several suppliers in the US that can satisfy probably all of your needs.
One is Ward's Scientific and the other is Carolina Biological. Their slides range from $3US to about $15US depending on subject.
gary g.
At 11:06 AM 11/28/2006, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Fri Dec 1 19:28:55 2006 10, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kB21SsEs019214 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 1 Dec 2006 19:28:55 -0600 10, 20 -- Received: (qmail 30313 invoked from network); 1 Dec 2006 17:28:48 -0800 10, 20 -- Received: by simscan 1.1.0 ppid: 30277, pid: 30308, t: 0.1694s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp1 with SMTP; 1 Dec 2006 17:28:47 -0800 10, 20 -- Message-Id: {7.0.1.0.2.20061201172643.025ecbc0-at-gaugler.com} 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 10, 20 -- Date: Fri, 01 Dec 2006 17:28:51 -0800 10, 20 -- To: blegge-at-cwisp.ca 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] AskAMicroscopist: source for OM sample slides 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200611281906.kASJ61Fq004292-at-ns.microscopy.com} 10, 20 -- References: {200611281906.kASJ61Fq004292-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Mary, That might work, but one problem is that sulfur likes metal surfaces and it is very difficult to get it off. There was some work on it at Sandia National Lab in the early 80's that I am familiar with.
John, if you go this route, you might want to heat the nitrogen before going into the system. Run your N2 through a coiled tubing with heat tape on it. That will help strip the sulfur from the surface. You just want to make sure that you are in the viscous flow range which Mary's suggestion will do. Doing both her suggestion and mine together with the copper getter in the system might be a good way to get the sulfur out. I think that you will have to go longer than her suggested hour, though, probably overnight. Sulfur really likes those surfaces and has a high heat of absorption.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: mager-at-interchange.ubc.ca [mailto:mager-at-interchange.ubc.ca] Sent: Friday, December 01, 2006 2:52 PM To: Walck-at-SouthBayTech.com
Dear Dr. Mardinly, I would suspect that the rubber in the seal that failed is the source of the sulphur and this will react with the copper strongly. Unfortunately, this will be distributed throughout the system by the oil back-streamed by the system failure and can only be removed by very thorough cleaning of all chambers and lines. You might try baking the system after cleaning with solvents to get rig of all the oil. Change the oil in the backing pump and run everything for a week to clean it up. The other thing I have used successfully to clean up an SEM that was contaminating badly was to bleed in a stream of nitrogen gas through a feed through I made with a bit of fine stainless steel tubing and a metering valve. The gas valve was turned on until the SEM baffle valve closed and a bit more. Leave for about one hour. Take a blank port cover, drill a small hole through, glue the SS tubing through the hole, swage the fine metering valve on to the SS tubing. Attach the other side of the metering valve to a bag you can fill with nitrogen gas. A bag fill will last more than an hour at the right gas flow rate.) You can use a freshly-polished piece of copper to test your system. Good luck, Mary Mager Electron Microscopist Materials Eng. UBC #419 - 6350 Stores Rd. Vancouver, B.C. V6T 1Z4 CANADA Phone: 604-822-5648 Fax: 604-822-3619 email: mager-at-interchange.ubc.ca
-----Original Message----- X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com] Sent: Friday, December 01, 2006 11:42 AM To: mager-at-interchange.ubc.ca
We have a Gatan Model 655 Dry Pumping Station (www.gatan.com/holders/655_drypumpingstation.html) equipped with pods for storage of TEM specimens. In particular, TEM cross-sections of integrated circuits using copper interconnects seem to be particularly vulnerable to corrosion in atmosphere. This vacuum station has been essential in protecting the specimens that we put so much work into making, and has been very successful for quite some time. The dry vacuum system consists of a 'drag' pump, which is essentially a turbo pump, backed by a diaphragm type rough pump. Last summer, one of the rubber diaphragms failed in the backing pump, and the system ran for a few days with degraded vacuum. After the diaphragms were replaced and the high vacuum restored to the E-5 Torr range, we found that TEM cross-sections of integrated circuits using copper interconnects were destroyed in just a few hours if stored in this vacuum system. EDX of the copper corrosion product showed the presence of sulfur. We have tried cleaning the storage pods, and part of the inside of the vacuum chamber, but have been totally frustrated in being able to restore the essential function of this vacuum storage system. Any suggestions as to what may have happened, and what we can do to clean up the system would be very much appreciated. Thank you.
John Mardinly Intel Corporation
Disclaimer: This is a communication from this author and does not represent an opinion of Intel Corporation.
==============================Original Headers============================== 6, 30 -- From john.mardinly-at-intel.com Fri Dec 1 13:35:49 2006 6, 30 -- Received: from mga01.intel.com (mga01.intel.com [192.55.52.88]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB1JZnHb024448 6, 30 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 1 Dec 2006 13:35:49 -0600 6, 30 -- Received: from fmsmga001.fm.intel.com ([10.253.24.23]) 6, 30 -- by mga01.intel.com with ESMTP; 01 Dec 2006 11:35:32 -0800 6, 30 -- Received: from scsmsx332.sc.intel.com (HELO scsmsx332.amr.corp.intel.com) ([10.3.90.6]) 6, 30 -- by fmsmga001.fm.intel.com with ESMTP; 01 Dec 2006 11:35:32 -0800 6, 30 -- X-ExtLoop1: 1 6, 30 -- X-IronPort-AV: i="4.09,486,1157353200"; 6, 30 -- d="scan'208"; a="171762165:sNHT19309206" 6, 30 -- Received: from scsmsx413.amr.corp.intel.com ([10.3.90.32]) by scsmsx332.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 6, 30 -- Fri, 1 Dec 2006 11:35:31 -0800 6, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 30 -- Content-class: urn:content-classes:message 6, 30 -- MIME-Version: 1.0 6, 30 -- Content-Type: text/plain; 6, 30 -- charset="us-ascii" 6, 30 -- Subject: Contamination of Vacuum Specimen Storage System 6, 30 --
Debby- Polyacrylamide is one of the few plastics I have never found a way to section. Things I would try are: 1. lower the temperature to cryosection it (maybe below -100 C, which works for rubber) 2. embed the sample just like a biological tissue.
It would be surprising if you saw much, anyway. Proteins in a gel will make a hairpin shape due to SDS binding. Knowledge of the sample would help you to know what to expect. Carol
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-- Carol A. Heckman, Ph.D. Director, Center for Microscopy & Microanalysis and Professor of Biological Sciences Bowling Green State University Bowling Green, OH 43403 website: http://www.bgsu.edu/departments/biology/facilities/MnM
==============================Original Headers============================== 4, 18 -- From heckman-at-bgnet.bgsu.edu Sat Dec 2 13:51:29 2006 4, 18 -- Received: from smtp01.bgsu.edu (smtp.bgsu.edu [129.1.5.17]) 4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB2JpT4G027372 4, 18 -- for {microscopy-at-microscopy.com} ; Sat, 2 Dec 2006 13:51:29 -0600 4, 18 -- Received: from [129.1.85.81] (dhcp-85-81.bgsu.edu [129.1.85.81]) 4, 18 -- by smtp01.bgsu.edu (Switch-3.1.8/Switch-3.1.6) with ESMTP id kB2JpHrH003372; 4, 18 -- Sat, 2 Dec 2006 14:51:18 -0500 (EST) 4, 18 -- Mime-Version: 1.0 4, 18 -- X-Sender: heckman-at-mailstore.bgsu.edu 4, 18 -- Message-Id: {p04320403c197b32d3f44-at-[129.1.85.81]} 4, 18 -- In-Reply-To: {200612011944.kB1JiaXS004163-at-ns.microscopy.com} 4, 18 -- References: {200612011944.kB1JiaXS004163-at-ns.microscopy.com} 4, 18 -- Date: Sat, 2 Dec 2006 14:51:15 -0800 4, 18 -- To: dsherman-at-purdue.edu 4, 18 -- From: Carol Heckman {heckman-at-bgnet.bgsu.edu} 4, 18 -- Subject: Re: [Microscopy] Embedding acrylamide gel 4, 18 -- Cc: "Microscopy Listserver" {microscopy-at-microscopy.com} 4, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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Email: cjlcwright-at-wildblue.net Name: Christopher Wright
Title-Subject: [Filtered] Leo32 Windows 98 Control Software
Question: Hi,
I am trying to help a customer with an older Zeiss microscope. The issue is with the Leo32 software (I am actually a software techie, not a microscope tech.)
He had someone supporting him in the past, but they never gave him a copy of the Leo32 software, nor the administrator password into the software. Of course, they are not returning his calls.
So now that he is having all these problems, I am limited in what I can accomplish.
Is there any where I can get a hold of the original software or any easy way to "hack" the administrator password? (This is with the microscope owner's permission of course.)
He is several hours away, so I have only had a little time to look at this. I thought everything was working so I focused on getting good backups of the software, but he is calling now and it is still giving problems, so my backups are worthless. I just need to start over, or hack into the administrator setup.
I quite share this opinion, however it really depends on the kind of information this person needs. I don't think the gel morphology is of interest, but more the nanoparticles themselves. In this case one does not have to care too much about what happens to the gel right? It is only supposition here, I am afraid.
Perhaps one could just fix the gel as Paul described it and dry it with light heating and vacuum (like for protein gels). Perhaps it would possible to get some interesting image in SEM (BSE should show a big contrast between the gel and the particles), but perhaps the resolution would be an issue (and depth too). An alternative would be to embed the dried gel in resin (if it is possible, I have no idea but you'll know it only if you try it) and cut it for TEM observation.
Stephane
--- paul_hazelton-at-umanitoba.ca wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Debbie, Bill, et al } } Been a couple of years since I've run an acrylamide } gel, either for } protein or sequencing. We fixed the gels in 30% } Isopropanol/10%Acetic } Acid prior to staining with coomassie } blue.Destaining involves Acetone } and Methanol, but i do not remember the precise } information on the } destaining solutions. After staining we destained } with } 16.5%Methanol/7.5% Acetic Acid. For sequencing I } used 7% Methanol/5% } Acetic acid. My memory is that some people used a } formaldehyde fixation } for sequencing gels, but I never tried that so do } not know how well it } would work. } } Regards cryofixation/sectioning, you could do that, } but it might be like } using a 100megaton thermonuclear device to remove a } tree stump. } Remember that acrylamide is a plastic. Standard } dehydration and } embedding would work quite well as long as the } target beads are held in } place. Caveat, have never done it, so really do not } know whether Bill } or I am right. Maybe you do need the bomb. } } This does not fit in our normal } formaldehyde/glutaraldehyde fixation but } it is consistent with fixation done for IF } microscopy } } ==============================Original } Headers============================== } 4, 21 -- From paul_hazelton-at-umanitoba.ca Fri Dec 1 } 19:17:31 2006 } 4, 21 -- Received: from electra.cc.umanitoba.ca } (electra.cc.umanitoba.ca [130.179.16.23]) } 4, 21 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } kB21HV28008406 } 4, 21 -- for {microscopy-at-microscopy.com} ; Fri, 1 } Dec 2006 19:17:31 -0600 } 4, 21 -- Received: from [130.179.152.89] } (cvx-026.cc.umanitoba.ca [130.179.152.89]) } 4, 21 -- (authenticated bits=0) } 4, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) } with ESMTP id kB21HQeb006824; } 4, 21 -- Fri, 1 Dec 2006 19:17:27 -0600 (CST) } 4, 21 -- Message-ID: {4570D421.50609-at-umanitoba.ca} } 4, 21 -- Date: Fri, 01 Dec 2006 19:17:21 -0600 } 4, 21 -- From: Paul Hazelton } {paul_hazelton-at-umanitoba.ca} } 4, 21 -- User-Agent: Mozilla/5.0 (Windows; U; } Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 } Netscape/7.2 (ax) } 4, 21 -- X-Accept-Language: en-us, en } 4, 21 -- MIME-Version: 1.0 } 4, 21 -- To: dsherman-at-purdue.edu, Microscopy } Listserver {microscopy-at-microscopy.com} , } 4, 21 -- Bill Tivol {tivol-at-caltech.edu} } 4, 21 -- Subject: Re: [Microscopy] Embedding } acrylamide gel } 4, 21 -- References: } {200612011945.kB1Jjmg0006334-at-ns.microscopy.com} } 4, 21 -- In-Reply-To: } {200612011945.kB1Jjmg0006334-at-ns.microscopy.com} } 4, 21 -- Content-Type: text/plain; } charset=ISO-8859-1; format=flowed } 4, 21 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== }
____________________________________________________________________________________ Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail beta. http://new.mail.yahoo.com
==============================Original Headers============================== 11, 20 -- From nizets2-at-yahoo.com Mon Dec 4 07:26:48 2006 11, 20 -- Received: from web37412.mail.mud.yahoo.com (web37412.mail.mud.yahoo.com [209.191.91.144]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kB4DQlti028778 11, 20 -- for {microscopy-at-microscopy.com} ; Mon, 4 Dec 2006 07:26:48 -0600 11, 20 -- Received: (qmail 35682 invoked by uid 60001); 4 Dec 2006 13:26:47 -0000 11, 20 -- Message-ID: {20061204132647.35680.qmail-at-web37412.mail.mud.yahoo.com} 11, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 20 -- s=s1024; d=yahoo.com; 11, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 11, 20 -- b=AhInLYdcrjbZSTlCY/HP9I2NGTp2/upI1A5ByIr9XD+zbzP+Py1Y6W4rbHeJ7cNcBpSpCMF6uEPOwI/+scIJrqh+KOn5sRviYHiDnzGdjI36FOK6dE7bOeM0A2RlEVZj2Brkx5aaZpsozsJBc8DFnAdKhH8hQqLOniIzvyLVE5g=; 11, 20 -- X-YMail-OSG: j16WOLwVM1m4WwPBeVNIoFEbHe9FRwcB0JDJ9bHUcADDINulx4fHT0ICnEksPPX4QdK3hygATNt2Ywlr60_5xURHARjlfIhbfoM2Y8L_N.VuABBvWoPmsKz.7zF6s8PXtdsUYEkZ813kB1aEB0qmdY1t_k.TQyJ.liIVQtogPLTjGEXS1qosYUplUCYBDTXYa9pKj8c6 11, 20 -- Received: from [80.122.101.102] by web37412.mail.mud.yahoo.com via HTTP; Mon, 04 Dec 2006 05:26:47 PST 11, 20 -- Date: Mon, 4 Dec 2006 05:26:47 -0800 (PST) 11, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 11, 20 -- Subject: Re: [Microscopy] Re: Embedding acrylamide gel 11, 20 -- To: microscopy-at-microscopy.com 11, 20 -- In-Reply-To: {200612020121.kB21LZQD016441-at-ns.microscopy.com} 11, 20 -- MIME-Version: 1.0 11, 20 -- Content-Type: text/plain; charset=iso-8859-1 11, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Hercules Incorporated is a global solutions provider of specialty chemicals and materials, with 4200 employees world-wide and annual sales of $2 billion. In our core businesses of Aqualon, Pulp and Paper, and Pinova, the Company serves a large number of markets including pulp and paper, paints, adhesives, construction materials, food, pharmaceuticals and personal care.
The Analytical & Engineering Science Division of Hercules Incorporated has an immediate opening for an experimental microscopist with a strong background in structural and morphological characterization of materials to join the R&D team at our Corporate Research Center in Wilmington, DE.
The successful candidate will be responsible for optical and electron microscopy characterization and method development in support of Hercules Incorporated R&D and manufacturing activities. As a member of the Analytical & Engineering Science Division, the candidate will collaborate on a diverse set of challenging technical problems in support of all of Hercules' business units. We are seeking a team player with excellent communication skills.
Hercules manufactures chemical specialty products used in a variety of home, office, and industrial products. The corporation's focus is on sustainable, long-term growth in shareholder value, driven by concentration on the customer, new product growth, and continuous improvement in manufacturing costs. For more information, visit the Hercules website at www.herc.com.
Requirements:
Advanced degree in chemistry, chemical engineering, biology, geology, or equivalent practical experience in experimental optical and/or electron microscopy. In depth knowledge of structural and/or morphological characterization using microscopy techniques. A broad background in Materials Science is preferred. Excellent oral and written communication skills. Permanent residency status in the U.S. is required.
As with all Hercules employment practices, this process will comply with all applicable equal employment laws. No candidate will be discriminated against on the basis of race, sex, national origin, color, religion, veteran status, disability, or any other protected class.
Qualified candidates are encouraged to send an application letter and a detailed resume to Hercules Incorporated, Research Center, Attn: Human Resources, 500 Hercules Road, Wilmington, DE 19808-1599 or technology-at-herc.com. Refer to position Microscopist.
==============================Original Headers============================== 13, 24 -- From cni-at-udel.edu Mon Dec 4 08:19:59 2006 13, 24 -- Received: from md4.nss.udel.edu (md4.nss.udel.edu [128.175.1.14]) 13, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB4EJwOs008057 13, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 4 Dec 2006 08:19:59 -0600 13, 24 -- Received: from Suba (em076-119.engg.udel.edu [128.175.119.76]) 13, 24 -- by md4.nss.udel.edu (MOS 3.8.2-GA) 13, 24 -- with ESMTP id DPS84448; 13, 24 -- Mon, 4 Dec 2006 09:19:58 -0500 (EST) 13, 24 -- From: "Chaoying Ni" {cni-at-udel.edu} 13, 24 -- To: {Microscopy-at-microscopy.com} 13, 24 -- Subject: Microscopist position at Hercules Incorporated 13, 24 -- Date: Mon, 4 Dec 2006 09:19:58 -0500 13, 24 -- Organization: University of Delaware 13, 24 -- Message-ID: {002e01c717af$475c85b0$4c77af80-at-Suba} 13, 24 -- MIME-Version: 1.0 13, 24 -- Content-Type: text/plain; 13, 24 -- charset="us-ascii" 13, 24 -- X-Priority: 3 (Normal) 13, 24 -- X-MSMail-Priority: Normal 13, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 13, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 13, 24 -- Importance: Normal 13, 24 -- Content-Transfer-Encoding: 8bit 13, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kB4EJwOs008057 ==============================End of - Headers==============================
Dear John, One other possibility would be the XEI Scientific, Inc. decontaminator. It uses oxygen to clean your system and sample and might be more vigorous than the nitrogen at removing the sulphur residue. I have no affiliation with XEI, nor do I own one. Regards, Mary Mager
-----Original Message----- X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com] Sent: Friday, December 01, 2006 11:42 AM To: mager-at-interchange.ubc.ca
We have a Gatan Model 655 Dry Pumping Station (www.gatan.com/holders/655_drypumpingstation.html) equipped with pods for storage of TEM specimens. In particular, TEM cross-sections of integrated circuits using copper interconnects seem to be particularly vulnerable to corrosion in atmosphere. This vacuum station has been essential in protecting the specimens that we put so much work into making, and has been very successful for quite some time. The dry vacuum system consists of a 'drag' pump, which is essentially a turbo pump, backed by a diaphragm type rough pump. Last summer, one of the rubber diaphragms failed in the backing pump, and the system ran for a few days with degraded vacuum. After the diaphragms were replaced and the high vacuum restored to the E-5 Torr range, we found that TEM cross-sections of integrated circuits using copper interconnects were destroyed in just a few hours if stored in this vacuum system. EDX of the copper corrosion product showed the presence of sulfur. We have tried cleaning the storage pods, and part of the inside of the vacuum chamber, but have been totally frustrated in being able to restore the essential function of this vacuum storage system. Any suggestions as to what may have happened, and what we can do to clean up the system would be very much appreciated. Thank you.
John Mardinly Intel Corporation
Disclaimer: This is a communication from this author and does not represent an opinion of Intel Corporation.
==============================Original Headers============================== 6, 30 -- From john.mardinly-at-intel.com Fri Dec 1 13:35:49 2006 6, 30 -- Received: from mga01.intel.com (mga01.intel.com [192.55.52.88]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB1JZnHb024448 6, 30 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 1 Dec 2006 13:35:49 -0600 6, 30 -- Received: from fmsmga001.fm.intel.com ([10.253.24.23]) 6, 30 -- by mga01.intel.com with ESMTP; 01 Dec 2006 11:35:32 -0800 6, 30 -- Received: from scsmsx332.sc.intel.com (HELO scsmsx332.amr.corp.intel.com) ([10.3.90.6]) 6, 30 -- by fmsmga001.fm.intel.com with ESMTP; 01 Dec 2006 11:35:32 -0800 6, 30 -- X-ExtLoop1: 1 6, 30 -- X-IronPort-AV: i="4.09,486,1157353200"; 6, 30 -- d="scan'208"; a="171762165:sNHT19309206" 6, 30 -- Received: from scsmsx413.amr.corp.intel.com ([10.3.90.32]) by scsmsx332.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 6, 30 -- Fri, 1 Dec 2006 11:35:31 -0800 6, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 30 -- Content-class: urn:content-classes:message 6, 30 -- MIME-Version: 1.0 6, 30 -- Content-Type: text/plain; 6, 30 -- charset="us-ascii" 6, 30 -- Subject: Contamination of Vacuum Specimen Storage System 6, 30 --
Advances in Electron Crystallography of Membrane Proteins: Call for papers for a special issue of JSB Ben Hankamer, Henning Stahlberg and Bob Glaeser, Guest Editors
The Journal of Structural Biology invites submissions for a special issue focusing on recent ‘Advances in Electron Crystallography of Membrane Proteins’. Electron-crystallography offers many advantages for the determination of membrane proteins structures, which is one of the great challenges of structural and cell biology.
In recent years electron-crystallography of membrane proteins has undergone major developments, which enhance its natural advantages. These advantages include the fact that membrane proteins can be crystallized within a near-native lipid bilayer environment, that 2-D crystals may sometimes be obtained even though 3-D crystals have not been, and that the technique is well suited for the crystallization of small and hydrophobic integral membrane proteins as well as membrane associated proteins. Functionalized monolayer technology is also opening up the possibility of streamlining crystal production and of coupling this process to improved protein expression techniques. As a resolution of better than 2Å has now been reported, it is proven that data can be collected to determine not only protein structure but its detailed interplay with the components of the lipid bilayer.
This special edition aims to capture the key developments that will help to take the electron crystallography field to the next phase, from streamlined crystal production and structure determination to new levels of resolution. Manuscripts in the following areas are specifically (but not exclusively) invited:
1. Membrane protein expression 2. Membrane protein purification 3. 2D crystallization 4. Sample preparation for electron microscopy 5. Automated screening of crystallization trials 6. Computer image processing and software developments 7. Recent applications and new structures
Papers can be in the form of research articles or mini-reviews that summarize key areas of importance. Prior to submission of a minireview, or if you require any further information on the suitability of your manuscript for inclusion in this special issue, please contact Ben Hankamer (b.hankamer-at-imb.uq.edu.au), Henning Stahlberg (HStahlberg-at-ucdavis.edu), or Bob Glaeser (RMGlaeser-at-lbl.gov) .
A brief e-mail by 22 Dec 2006, stating your intent to submit a contribution would be greatly appreciated as it would help us to plan the format of the special issue. The deadline for receipt of manuscripts for the Special Issue is 1 May 2006. Manuscripts will be peer-reviewed using the criteria of the Journal of Structural Biology (see http://www.academicpress.com/jsb). Submissions should be made on-line via the normal JSB submission process. In the cover letter please state that you wish the article to be considered for the Special Issue on ‘Advances in Electron Crystallography’ and mark for the attention of Ben Hankamer, Henning Stahlberg or Bob Glaeser.
_____________________________________________________________ Henning Stahlberg, Molecular & Cellular Biology, Briggs Hall 5, University of California at Davis, 1 Shields Ave., Davis, CA 95616, USA Tel: +1-530-752 8282 (office), +1-530-754 8285 (lab) Fax: +1-530-752 3085 mailto:HStahlberg-at-ucdavis.edu http://stahlberglab.ucdavis.edu http://2dx.org _____________________________________________________________
==============================Original Headers============================== 17, 21 -- From HStahlberg-at-ucdavis.edu Mon Dec 4 12:47:54 2006 17, 21 -- Received: from warsaw.ucdavis.edu (warsaw.ucdavis.edu [128.120.32.41]) 17, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB4IlrhL006058 17, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 4 Dec 2006 12:47:53 -0600 17, 21 -- Received: from [169.237.214.65] ([169.237.214.65]) 17, 21 -- by warsaw.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id kB4IlJdD026384; 17, 21 -- Mon, 4 Dec 2006 10:47:19 -0800 (PST) 17, 21 -- Mime-Version: 1.0 (Apple Message framework v752.2) 17, 21 -- Content-Type: text/plain; charset=WINDOWS-1252; delsp=yes; format=flowed 17, 21 -- Message-Id: {FAD32BC8-BB32-48DE-80D6-32673C0C00B5-at-ucdavis.edu} 17, 21 -- Cc: Ben Hankamer {b.hankamer-at-imb.uq.edu.au} , 17, 21 -- Henning Stahlberg {HStahlberg-at-ucdavis.edu} , 17, 21 -- "Robert M. Glaeser" {RMGlaeser-at-lbl.gov} 17, 21 -- From: Henning Stahlberg {HStahlberg-at-ucdavis.edu} 17, 21 -- Subject: Advances in Electron Crystallography of Membrane Proteins: Call for papers for a special issue of JSB 17, 21 -- Date: Mon, 4 Dec 2006 10:47:18 -0800 17, 21 -- To: Microscopy-at-microscopy.com 17, 21 -- X-Mailer: Apple Mail (2.752.2) 17, 21 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.41 17, 21 -- Content-Transfer-Encoding: 8bit 17, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kB4IlrhL006058 ==============================End of - Headers==============================
Many thanks for all the replies. Your suggestions and experiences give me some more things to think about and run past the service engineers and users (who are being very cooperative).
I should have mentioned in my original post that this system is dry pumped so pumping oil is not in the picture (I don't know why I did not think to mention that since that would have been one of my first questions).
Several of you have suggested that I have the contamination analyzed with FTIR and I am in the process of arranging that.
Again, many thanks and I will let you know about any thing that comes to light.
Tom
Tom Stephens Assistant Research Scientist Microscopy and Imaging Center Texas A&M University College Station, TX 77843 office phone:979-845-1165 email:tstephen-at-mic.tamu.edu
==============================Original Headers============================== 8, 20 -- From tstephen-at-mic.tamu.edu Mon Dec 4 15:17:57 2006 8, 20 -- Received: from sr-5-int.cis.tamu.edu (smtp-relay.tamu.edu [165.91.22.120]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB4LHv2n020145 8, 20 -- for {Microscopy-at-Microscopy.Com} ; Mon, 4 Dec 2006 15:17:57 -0600 8, 20 -- Received: from localhost (localhost.tamu.edu [127.0.0.1]) 8, 20 -- by sr-5-int.cis.tamu.edu (Postfix) with ESMTP id 5D27F70EC 8, 20 -- for {Microscopy-at-Microscopy.Com} ; Mon, 4 Dec 2006 15:17:57 -0600 (CST) 8, 20 -- Received: from jntcspc.mic.tamu.edu (cyn6400.tamu.edu [165.91.109.143]) 8, 20 -- by sr-5-int.cis.tamu.edu (Postfix) with ESMTP id 9DEEC70E2 8, 20 -- for {Microscopy-at-Microscopy.Com} ; Mon, 4 Dec 2006 15:17:56 -0600 (CST) 8, 20 -- Message-Id: {5.2.0.9.0.20061201122251.02a969f0-at-mic.tamu.edu} 8, 20 -- X-Sender: tstephen-at-mic.tamu.edu (Unverified) 8, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.0.9 8, 20 -- Date: Mon, 04 Dec 2006 15:18:06 -0600 8, 20 -- To: Microscopy-at-Microscopy.Com 8, 20 -- From: Tom Stephens {tstephen-at-mic.tamu.edu} 8, 20 -- Subject: Re: Re: [Microscopy] SEM - Mystery contamination of FESEM 8, 20 -- Mime-Version: 1.0 8, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 20 -- X-Virus-Scanned: amavisd-new at tamu.edu ==============================End of - Headers==============================
Hello, I have noticed strange behavior of the Emission Meter of our Philips CM100 electron microscope. When the microscope is ON with HT switched OFF, the pointer of the meter is in the leftmost position indicating negative current!? It does not change even when HT is switched ON (40kV, 60kV, 80kV, 100kV). No change can also be observed when the filament is heated; the pointer stuck in leftmost position. However, when the microscope is in STAND BY, the pointer is in normal position indicating to zero.
I have changed the whenelt for the other one with a new cathode, but it did not help. Does anybody has an explanation for this? Thanking you in advance for any suggestion.
Oldrich
------------------------------------------ Oldrich Benada Institute of Microbiology Acad. Sci. CR Videnska 1083 CZ-142 20 Prague 4 Czech Republic
==============================Original Headers============================== 4, 20 -- From benada-at-biomed.cas.cz Tue Dec 5 08:20:58 2006 4, 20 -- Received: from biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB5EKvAF021183 4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 5 Dec 2006 08:20:58 -0600 4, 20 -- Received: from [147.231.44.101] (u117ob.mbu.cas.cz [147.231.44.101]) 4, 20 -- by biomed.cas.cz (8.13.3+Sun/8.13.3) with ESMTP id kB5EJvVf008075 4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 5 Dec 2006 15:20:01 +0100 (CET) 4, 20 -- From: benada-at-biomed.cas.cz 4, 20 -- To: microscopy-at-microscopy.com 4, 20 -- Date: Tue, 05 Dec 2006 15:20:48 +0100 4, 20 -- MIME-Version: 1.0 4, 20 -- Subject: Emission Meter - Philips CM100 4, 20 -- Message-ID: {45758E50.10737.568DD6-at-benada.biomed.cas.cz} 4, 20 -- Priority: normal 4, 20 -- X-mailer: Pegasus Mail for Windows (4.41) 4, 20 -- Content-type: text/plain; charset=US-ASCII 4, 20 -- Content-transfer-encoding: 7BIT 4, 20 -- Content-description: Mail message body 4, 20 -- X-Antivirus: avast! (VPS 0653-5, 05.12.2006), Outbound message 4, 20 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
I'm having a weird problem with the LN2 dipstick on my EDS detector. Background - this is a PGT Avalon system that uses an interface box between the dipstick and the Avalon electronics. The dipstick has a 1K ohm resister in the tip and a potentiometer in the interface box is used to set the low LN2 point. The low LN2 point is set correctly and when the thing is working correctly, removing the stick from LN2 will shut off the HV.
Here is the problem. When I drop the dipstick into the EDS detector dewar (full of LN2) the ratemeter on the Avalon electronics pegs to 100% deadtime. If I take it out the ratemeter the deadtime goes back to near 0. Repeating yields same results. Here is the mind bender - if I drop the dipstick into a vacuum flask full of LN2 the ratemeter stays at ~0% deadtime.
I have done the following;
Bypassed the dipstick and cabling by jumpering a 1k ohm resistor directly into the interface box. I drop the resistor into LN2 and I see no noise in ratemeter. Next I added back in the cable with the resistor, dip it into the dewar flask and it works fine. Next I add the dipstick and cable and they work fine. Next I drop the dipstick ( connected to interface box by cable) into the detector dewar and the ratemeter pegs 100% DT.
I've rebuilt the dipstick - new resistor, and wiring. New cable from dipstick to interface box. Still same problem.
Any ideas??
Owen Mills Michigan Tech University
} all done w/ no ebeam. } } 1. with dipstick in detector dewar DT=100%. } 2. remove dipstick and DT drops to ~0. } 3. this happens every time. } 4. drop dipstick into flask of ln2 - ~ 0% DT } } so what is different about the detector dewar? } } btw, if you drop a 1k ohm resistor into ln2 the resistance goes up } to 1.7k ohm. } } let me know if you can figure this out. } } Owen }
==============================Original Headers============================== 14, 31 -- From opmills-at-mtu.edu Tue Dec 5 09:04:09 2006 14, 31 -- Received: from node8.mtu.edu (node8.mtu.edu [141.219.69.8]) 14, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB5F49DS000647 14, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 5 Dec 2006 09:04:09 -0600 14, 31 -- Received: from node6.edge.dcsint.mtu.edu (node6.mtu.edu [141.219.69.6]) 14, 31 -- by node8.mtu.edu (8.13.1/8.13.1) with ESMTP id kB5F49Z1028389 14, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 5 Dec 2006 10:04:09 -0500 14, 31 -- Received: from node23.edge.dcsint.mtu.edu (node23.mtu.edu [141.219.69.23]) 14, 31 -- by node6.edge.dcsint.mtu.edu (8.12.11.20060308/8.12.8) with ESMTP id kB5F49qL013649 14, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 5 Dec 2006 10:04:09 -0500 14, 31 -- Received: from mail.mtu.edu (campus0.mtu.edu [141.219.70.8]) 14, 31 -- by node23.edge.dcsint.mtu.edu (8.12.11.20060614/8.12.11) with ESMTP id kB5F485b021445 14, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 5 Dec 2006 10:04:08 -0500 14, 31 -- (envelope-from opmills-at-mtu.edu) 14, 31 -- Received: from node6.edge.dcsint.mtu.edu (node6.mtu.edu [141.219.69.6]) 14, 31 -- by mail.mtu.edu (8.13.6+Sun/8.11.6) with ESMTP id kB5F47MF010989 14, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 5 Dec 2006 10:04:07 -0500 (EST) 14, 31 -- Received: from [141.219.192.45] (opmills.eecn.sabo.mtu.edu [141.219.192.45]) 14, 31 -- by node6.edge.dcsint.mtu.edu (8.12.11.20060308/8.12.3/auth_ssl.mc v1.0) with ESMTP id kB5F47GK013637 14, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 5 Dec 2006 10:04:07 -0500 14, 31 -- Mime-Version: 1.0 (Apple Message framework v752.3) 14, 31 -- Content-Transfer-Encoding: 7bit 14, 31 -- Message-Id: {16BADD7E-DC55-4F45-80E3-EE2B827AD956-at-mtu.edu} 14, 31 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 14, 31 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 14, 31 -- From: "Owen P. Mills" {opmills-at-mtu.edu} 14, 31 -- Subject: EDS dipstick conundrum 14, 31 -- Date: Tue, 5 Dec 2006 10:04:06 -0500 14, 31 -- X-Mailer: Apple Mail (2.752.3) 14, 31 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2006.12.5.63432 14, 31 -- X-PerlMx-Spam: Gauge=IIIIIII, Probability=7%, Report='__CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
If emission control still works, then troubleshoot emission meter circuit. It is hard to be more specific at this point. I don't think that cleaning and column alignment will help.
Vitaly Feingold SIA 2773 Heath Line Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com
----- Original Message ----- X-from: {benada-at-biomed.cas.cz} To: {vitalylazar-at-att.net} Sent: Tuesday, December 05, 2006 9:24 AM
Oldrich,
We have a Philips CM200 with similar issues with the emission meter. Our service engineer has told us that the emission meter has had a history of problems. His suggestion is to use CM Remote to read the emission current to an external PC.
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Hello, I have noticed strange behavior of the Emission Meter of our Philips CM100 electron microscope. When the microscope is ON with HT switched OFF, the pointer of the meter is in the leftmost position indicating negative current!? It does not change even when HT is switched ON (40kV, 60kV, 80kV, 100kV). No change can also be observed when the filament is heated; the pointer stuck in leftmost position. However, when the microscope is in STAND BY, the pointer is in normal position indicating to zero.
I have changed the whenelt for the other one with a new cathode, but it did not help. Does anybody has an explanation for this? Thanking you in advance for any suggestion.
Oldrich
------------------------------------------ Oldrich Benada Institute of Microbiology Acad. Sci. CR Videnska 1083 CZ-142 20 Prague 4 Czech Republic
==============================Original Headers============================== 12, 23 -- From david.r.hull-at-nasa.gov Tue Dec 5 12:19:06 2006 12, 23 -- Received: from ndjsvws04.ndc.nasa.gov (ndjsvws04.ndc.nasa.gov [198.120.25.84]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB5IJ5oo028662 12, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 5 Dec 2006 12:19:05 -0600 12, 23 -- Received: from ndjsxgw01.ndc.nasa.gov ([129.166.32.111]) by ndjsvws04.ndc.nasa.gov with InterScan Messaging Security Suite; Tue, 05 Dec 2006 12:19:04 -0600 12, 23 -- Received: from NDJSEVS16.ndc.nasa.gov ([129.166.32.126]) by ndjsxgw01.ndc.nasa.gov with Microsoft SMTPSVC(6.0.3790.1830); 12, 23 -- Tue, 5 Dec 2006 12:19:02 -0600 12, 23 -- Received: from 129.166.32.15 ([129.166.32.15]) by NDJSEVS16.ndc.nasa.gov ([129.166.32.136]) via Exchange Front-End Server mail01.ndc.nasa.gov ([129.166.32.105]) with Microsoft Exchange Server HTTP-DAV ; 12, 23 -- Tue, 5 Dec 2006 18:19:02 +0000 12, 23 -- User-Agent: Microsoft-Entourage/11.2.5.060620 12, 23 -- Date: Tue, 05 Dec 2006 13:21:09 -0500 12, 23 -- Subject: FW: [Microscopy] Emission Meter - Philips CM100 12, 23 -- From: "Hull, David R" {David.R.Hull-at-nasa.gov} 12, 23 -- To: {Microscopy-at-microscopy.com} 12, 23 -- Message-ID: {C19B22C5.2127%David.R.Hull-at-nasa.gov} 12, 23 -- Thread-Topic: [Microscopy] Emission Meter - Philips CM100 12, 23 -- Thread-Index: AccYmiK6YWXUH4SNEduBcAAKldSx3A== 12, 23 -- In-Reply-To: {200612051422.kB5EMcGw022425-at-ns.microscopy.com} 12, 23 -- Mime-version: 1.0 12, 23 -- Content-type: text/plain; 12, 23 -- charset="US-ASCII" 12, 23 -- Content-transfer-encoding: 7bit 12, 23 -- X-OriginalArrivalTime: 05 Dec 2006 18:19:02.0417 (UTC) FILETIME=[D7471010:01C71899] ==============================End of - Headers==============================
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Email: rra-at-stowers-institute.org Name: Rhonda Allen
Organization: Stowers Institute
Title-Subject: [Filtered] Leica EM-Stain
Question: Hello, I am interested in purchasing the Leica EM-Stain. Does anyone have any comments, positive and negative, that I should take into consideration before making such an investment? We will be using it on formvar coated slot grids. Thanks in advance, Rhonda Allen BA HT(ASCP)HTL, QIHC Stowers Institute for Medical Research Kansas City, Missouri 816-926-4346
Earth loop causing noise pickup etc into your analyser? When you put the dipstick into the detector dewar you may be inadvertently closing the loop somehow? Look for exposed or broken earthing braid etc?
Arthur.
} } All, } } I'm having a weird problem with the LN2 dipstick on my EDS detector. } Background - this is a PGT Avalon system that uses an interface box } between the dipstick and the Avalon electronics. The dipstick has a } 1K ohm resister in the tip and a potentiometer in the interface box } is used to set the low LN2 point. The low LN2 point is set correctly } and when the thing is working correctly, removing the stick from LN2 } will shut off the HV. } } Here is the problem. When I drop the dipstick into the EDS detector } dewar (full of LN2) the ratemeter on the Avalon electronics pegs to } 100% deadtime. If I take it out the ratemeter the deadtime goes back } to near 0. Repeating yields same results. Here is the mind bender - } if I drop the dipstick into a vacuum flask full of LN2 the ratemeter } stays at ~0% deadtime. } } I have done the following; } } Bypassed the dipstick and cabling by jumpering a 1k ohm resistor } directly into the interface box. I drop the resistor into LN2 and I } see no noise in ratemeter. Next I added back in the cable with the } resistor, dip it into the dewar flask and it works fine. Next I add } the dipstick and cable and they work fine. Next I drop the dipstick } ( connected to interface box by cable) into the detector dewar and } the ratemeter pegs 100% DT. } } I've rebuilt the dipstick - new resistor, and wiring. New cable from } dipstick to interface box. Still same problem. } } Any ideas?? } } Owen Mills } Michigan Tech University } } } } } } } } all done w/ no ebeam. } } } } 1. with dipstick in detector dewar DT=100%. } } 2. remove dipstick and DT drops to ~0. } } 3. this happens every time. } } 4. drop dipstick into flask of ln2 - ~ 0% DT } } } } so what is different about the detector dewar? } } } } btw, if you drop a 1k ohm resistor into ln2 the resistance goes up } } to 1.7k ohm. } } } } let me know if you can figure this out. } } } } Owen } } }
==============================Original Headers============================== 5, 27 -- From ard-at-ansto.gov.au Tue Dec 5 19:01:12 2006 5, 27 -- Received: from tachyon.gw.ansto.gov.au (tachyon.gw.ansto.gov.au [137.157.8.253]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB611AMs028183 5, 27 -- for {microscopy-at-microscopy.com} ; Tue, 5 Dec 2006 19:01:11 -0600 5, 27 -- Received: (from uucp-at-localhost) 5, 27 -- by tachyon.gw.ansto.gov.au (8.11.7p1+Sun/8.11.7) id kB6103o16961; 5, 27 -- Wed, 6 Dec 2006 12:00:03 +1100 (EST) 5, 27 -- Received: from jenner.ansto.gov.au(137.157.59.25) by tachyon.gw.ansto.gov.au via csmap (V6.0) 5, 27 -- id srcCAA5OaOeH; Wed, 6 Dec 06 12:00:03 +1100 5, 27 -- Received: from hadron.ansto.gov.au (hadron.ansto.gov.au [137.157.13.219]) 5, 27 -- by jenner.ansto.gov.au (8.12.10/8.12.10) with ESMTP id kB60umVr026928; 5, 27 -- Wed, 6 Dec 2006 11:56:48 +1100 5, 27 -- Received: from [137.157.95.82] (arthur.amat.ansto.gov.au [137.157.95.82]) 5, 27 -- by hadron.ansto.gov.au (8.9.3/8.9.3) with ESMTP id LAA26475; 5, 27 -- Wed, 6 Dec 2006 11:56:42 +1100 (EST) 5, 27 -- Mime-Version: 1.0 5, 27 -- X-Sender: ard-at-hadron.ansto.gov.au 5, 27 -- Message-Id: {v04210100c19bc495d087-at-[137.157.95.82]} 5, 27 -- In-Reply-To: {200612051505.kB5F54ks002572-at-ns.microscopy.com} 5, 27 -- References: {200612051505.kB5F54ks002572-at-ns.microscopy.com} 5, 27 -- Date: Wed, 6 Dec 2006 11:56:42 +1100 5, 27 -- To: {microscopy-at-microscopy.com} 5, 27 -- From: Arthur Day {ard-at-ansto.gov.au} 5, 27 -- Subject: Re: [Microscopy] EDS dipstick conundrum 5, 27 -- Cc: opmills-at-mtu.edu 5, 27 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 27 -- X-ANSTO-MailScanner: Found to be clean ==============================End of - Headers==============================
There is a possibility the problem is not electrical. The detector may have become more microphonic (or more heat is being liberated by the dipstick).
In this scenario, the dipstick causes boiling of the LN2 and the vibration is causing the deadtime increase. To test this, simply insert a room temperature metal rod to the same depth (e.g. a welding rod or two).
If the DT increases, boiling vibes are likely the trouble. If not, it is probably a (somehow) ground loop.
Good luck & best regards,
Woody White BWXT Services
==============================Original Headers============================== 9, 26 -- From nwwhite-at-bwxt.com Wed Dec 6 07:03:26 2006 9, 26 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB6D3PNV015277 9, 26 -- for {microscopy-at-msa.microscopy.com} ; Wed, 6 Dec 2006 07:03:26 -0600 9, 26 -- Received: from ([131.184.13.224]) 9, 26 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.3073251; 9, 26 -- Wed, 06 Dec 2006 08:03:03 -0500 9, 26 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); 9, 26 -- Wed, 6 Dec 2006 08:03:03 -0500 9, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 26 -- Content-class: urn:content-classes:message 9, 26 -- MIME-Version: 1.0 9, 26 -- Content-Type: text/plain; 9, 26 -- charset="us-ascii" 9, 26 -- Subject: RE: [Microscopy] EDS dipstick conundrum 9, 26 -- Date: Wed, 6 Dec 2006 08:03:02 -0500 9, 26 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB703D0E5-at-BWXSPO01.BWXS.BWXTECH.NET} 9, 26 -- X-MS-Has-Attach: 9, 26 -- X-MS-TNEF-Correlator: 9, 26 -- Thread-Topic: [Microscopy] EDS dipstick conundrum 9, 26 -- Thread-Index: AccYfrnYz830PGqvRPCOCPE8U2zxTgAtuHIg 9, 26 -- From: "White, Woody N." {NWWhite-at-bwxt.com} 9, 26 -- To: {opmills-at-mtu.edu} , "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 9, 26 -- X-OriginalArrivalTime: 06 Dec 2006 13:03:03.0316 (UTC) FILETIME=[DD2F9540:01C71936] 9, 26 -- Content-Transfer-Encoding: 8bit 9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kB6D3PNV015277 ==============================End of - Headers==============================
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Email: vthawfeek-at-yahoo.co.in Name: V.Thawfeek Mohammed
Organization: psg tech coll. of engg.
Title-Subject: [Filtered] optical physics
Question: why does a light beam diverge when it strikes a medium with greater refractive index than the medium ,in which it is.
unfortunately I am neither a light beam nor a medium with greater or smaller refractive index than the other medium.....so I can't tell you (truly) why (:-)) ...but I think you should have in mind the "refraction law" of Snellius (Snel's - Snell's - Snellius' Law, refraction law, law of refractive index) which is defined as:
n times sin alpha = n' times sin.ß
(I try to translate a text from German, apologize if not all terms are correct "English" : a ray/light beam incident under a certain angle } alpha { (with regard to the area of entry = incidence perpendicle) to the border/boundary of two optic media with refraction index n and n' respectively, will be bent/refracted. The resultant beam will transit through the second medium with refractory index n' under a certain angle ß with regard to the incidence perpendicle).
search Google for either term of SNELLIUS (see above) or directly ==} http://en.wikipedia.org/wiki/Snell's_law Hope this helps best regards,
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Question: why does a light beam diverge when it strikes a medium with greater refractive index than the medium, in which it is.
Scott; Thanks to you and everyone that responded with their thoughts on this problem. The feeling among our group now is that your thought that outgassing from the grease in the mechanical part of the diaphragm pumps can backstream when the diaphragms fail is the most likely diagnosis. This particular pump had a huge tear in the failed diaphragm, but this was a bit of a surprise, since our many Gatan PIPS have exactly the same pumps, and we routinely run them until diaphragm failure, although they tend to fail with smaller tears, and perhaps the gas load from the argon ion guns tends to keep them cleaner by purging backstreaming. We have cleaned the vacuum chamber with solvents (and it was a mess), but cleaning the turbo pump will be difficult, since it cannot be disassembled. A nitrogen purge will probably be the best method of cleaning the turbo. We also now intend to proactively change all of our diaphragms on an annual basis, rather than running them to failure, in the hope that this sort of mess never recurs.
John Mardinly Intel Corporation
Disclaimer: This is a communication from this author and does not represent an opinion of Intel Corporation.
-----Original Message----- X-from: Scott Walck [mailto:walck-at-southbaytech.com] Sent: Friday, December 01, 2006 2:43 PM To: Microscopy-at-microscopy.com Cc: Mardinly, John
We have a Gatan Model 655 Dry Pumping Station (www.gatan.com/holders/655_drypumpingstation.html) equipped with pods for storage of TEM specimens. In particular, TEM cross-sections of integrated circuits using copper interconnects seem to be particularly vulnerable to corrosion in atmosphere. This vacuum station has been essential in protecting the specimens that we put so much work into making, and has been very successful for quite some time. The dry vacuum system consists of a 'drag' pump, which is essentially a turbo pump, backed by a diaphragm type rough pump. Last summer, one of the rubber diaphragms failed in the backing pump, and the system ran for a few days with degraded vacuum. After the diaphragms were replaced and the high vacuum restored to the E-5 Torr range, we found that TEM cross-sections of integrated circuits using copper interconnects were destroyed in just a few hours if stored in this vacuum system. EDX of the copper corrosion product showed the presence of sulfur. We have tried cleaning the storage pods, and part of the inside of the vacuum chamber, but have been totally frustrated in being able to restore the essential function of this vacuum storage system. Any suggestions as to what may have happened, and what we can do to clean up the system would be very much appreciated. Thank you.
John Mardinly Intel Corporation
Disclaimer: This is a communication from this author and does not represent an opinion of Intel Corporation.
==============================Original Headers============================== 6, 30 -- From john.mardinly-at-intel.com Fri Dec 1 13:35:49 2006 6, 30 -- Received: from mga01.intel.com (mga01.intel.com [192.55.52.88]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB1JZnHb024448 6, 30 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 1 Dec 2006 13:35:49 -0600 6, 30 -- Received: from fmsmga001.fm.intel.com ([10.253.24.23]) 6, 30 -- by mga01.intel.com with ESMTP; 01 Dec 2006 11:35:32 -0800 6, 30 -- Received: from scsmsx332.sc.intel.com (HELO scsmsx332.amr.corp.intel.com) ([10.3.90.6]) 6, 30 -- by fmsmga001.fm.intel.com with ESMTP; 01 Dec 2006 11:35:32 -0800 6, 30 -- X-ExtLoop1: 1 6, 30 -- X-IronPort-AV: i="4.09,486,1157353200"; 6, 30 -- d="scan'208"; a="171762165:sNHT19309206" 6, 30 -- Received: from scsmsx413.amr.corp.intel.com ([10.3.90.32]) by scsmsx332.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 6, 30 -- Fri, 1 Dec 2006 11:35:31 -0800 6, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 30 -- Content-class: urn:content-classes:message 6, 30 -- MIME-Version: 1.0 6, 30 -- Content-Type: text/plain; 6, 30 -- charset="us-ascii" 6, 30 -- Subject: Contamination of Vacuum Specimen Storage System 6, 30 --
Electron Microscopy Specialist The Stowers Institute for Medical Research has an opening for an Electron Microscopy (EM) Specialist in the Histology Department. Responsibilities include providing high quality research EM services using established protocols; daily operation of the electron microscopy lab; using and maintaining the microscopes and ancillary equipment; assisting researchers with specimen preparation; sample preparation, including sample receipt, fixation, processing, and embedding; development of protocols; training SIMR research members and other users of techniques and equipment; development of seminars and/or presentations for internal training; purchasing supplies; and maintaining records/archives of the EM lab operation. In addition to excellent organizational, communication, and problem solving skills, the successful candidate should be familiar with operation of all applicable specimen preparation equipment and microscopes (TEM and SEM); skilled at standard specimen preparation protocols and identifying associated artifacts; have experience in high pressure freezing, cryoEM techniques, and immuno EM; be able to lift in excess of 30 pounds; and be able work overtime, including weekday, weekend, or on call work. The minimum requirements include an Associates Degree in a biological science and four years experience in research EM. Or a four year degree in a biological science and two years experience in research EM. Check out all our many benefits at www.stowers-institute.org. Rhonda Allen BA HT(ASCP)HTL, QIHC Histotechnology Specialist II Stowers Institute 1000 E. 50th Street Kansas City, MO 64110 816-926-4305
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Mohammed, If memory serves me, the bending occurs due to a change in the speed of light in different materials. A higher refractive index indicates a lower speed.
The speed of light that is immutable, according to Einstein, is the speed of light in a vacuum.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
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Email: vthawfeek-at-yahoo.co.in Name: V.Thawfeek Mohammed
Organization: psg tech coll. of engg.
Title-Subject: [Filtered] optical physics
Question: why does a light beam diverge when it strikes a medium with greater refractive index than the medium ,in which it is.
The answer is really quite simple: Snell's Law. The bending of light as it crosses the boundary from one RI to another is governed by the Laws of Refraction.
The bending discussed in your question requires that several conditions be met: First, that there be different RIs on each side of the boundary. Second, that the light approach the boundary at an angle.
To understand what happens, it is first necessary to understand that refractive index is actually a measure of the impact of interaction between the electric field of light and the electric field of matter. The greater the interaction, the more slowly light will travel through that material and the higher the refractive index. For instance: RI = velocity of light in air/velocity of light in material RI of air = 1, velocity of light = 300,000 km/s RI of water is 1.33, velocity of light is 225,000 km/s RI of immersion oil, glass, and many polymers is approximately 1.5, velocity of light = 200,000 km/sec.
As for refraction, the analogy which is often used is that of a person roller skating (or in this day and age, roller blading) from one surface to another. For example, from the concrete sidewalk onto grass. The smooth surface of the concrete is analogous to a material with low refractive index; the rougher surface of glass is analogous to a material with higher RI. If the skater approaches the sidewalk:grass boundary with both feet parallel, both feet will slow down by the same amount and the skater will continue skating in the same direction. However, if the skater approaches the boundary at an angle, one foot will slow down while the other remains at the original speed. That different in speed causes the skater to pivot toward the material of higher RI.
If you think of light in terms of a wave front rather than a simple ray, the analogy transfers easily. If the wave approaches a boundary at an angle, the edge of the front which hits the higher RI first will slow down, causing the whole wave front to pivot around that point. That's what happens, for example, when light travels from air (RI = 1.00) into a glass coverslip (RI ~1.5) or a droplet of water or a cell (RI ~1.33). You can use the optical axis of the microscope as a reference. In these cases, the light will bend in TOWARD the optical axis. Conversely, when light emerges from a glass slide or a droplet of water it will bend AWAY from the OA. Actually this is why we use oil immersion: to cause those rays which would normally bend away from the OA, causing a loss of both intensity and the critical contribution to resolution and edge definition, to bend back toward the OA, where they have an opportunity to be captured by the objective and make a positive contribution to better imaging.
All of this is explained in detail, with diagrams in Optimizing Light Microscopy. If you are interested in a copy, please contact Ken Piel here in the MME office for purchasing details (see below).
Hope this was helpful.
Best regards, Barbara Foster
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
MME is now scheduling customized, on-site courses through next April. Call us today for details.
P. S. Need a good general reference or light microscopy text for the Spring semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011 or email him at kenpiel-at-mme1.com
At 08:24 AM 12/6/2006, vthawfeek-at-yahoo.co.in wrote:
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==============================Original Headers============================== 20, 17 -- From bfoster-at-mme1.com Wed Dec 6 11:21:18 2006 20, 17 -- Received: from 5starpro.com (enterprise.5starpro.com [207.44.136.95]) 20, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB6HLIZk027160 20, 17 -- for {microscopy-at-microscopy.com} ; Wed, 6 Dec 2006 11:21:18 -0600 20, 17 -- Received: (qmail 23303 invoked by uid 2020); 6 Dec 2006 11:44:03 -0600 20, 17 -- Received: from host57-99.rancor.birch.net (HELO barbsd505.mme1.com) (65.17.57.99) 20, 17 -- by with (DHE-RSA-AES256-SHA encrypted) SMTP; 6 Dec 2006 11:44:02 -0600 20, 17 -- Message-Id: {7.0.1.0.0.20061206110350.01e57160-at-mme1.com} 20, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 20, 17 -- Date: Wed, 06 Dec 2006 11:20:51 -0600 20, 17 -- To: vthawfeek-at-yahoo.co.in, microscopy-at-microscopy.com 20, 17 -- From: Barbara Foster {bfoster-at-mme1.com} 20, 17 -- Subject: Re: [Microscopy] viaWWW: optical physics 20, 17 -- In-Reply-To: {200612061419.kB6EJhin013008-at-ns.microscopy.com} 20, 17 -- References: {200612061419.kB6EJhin013008-at-ns.microscopy.com} 20, 17 -- Mime-Version: 1.0 20, 17 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
OLYMPUS SOFT IMAGING SOLUTIONS 12596 West Bayaud Ave #300 Lakewood, CO 80228 USA Tel.: +1 (303) 234-9270 Fax.: +1 (303) 234-9271 E-mail: Mike.Bode-at-olympus-sis.com www.olympus-sis.com
-----Original Message----- X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] Sent: Wednesday, December 06, 2006 10:07 To: Mike Bode
Mohammed, If memory serves me, the bending occurs due to a change in the speed of light in different materials. A higher refractive index indicates a lower speed.
The speed of light that is immutable, according to Einstein, is the speed of light in a vacuum.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: vthawfeek-at-yahoo.co.in [mailto:vthawfeek-at-yahoo.co.in] Sent: Wednesday, December 06, 2006 9:20 AM To: kenconverse-at-qualityimages.biz
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying
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Email: vthawfeek-at-yahoo.co.in Name: V.Thawfeek Mohammed
Organization: psg tech coll. of engg.
Title-Subject: [Filtered] optical physics
Question: why does a light beam diverge when it strikes a medium with greater refractive index than the medium ,in which it is.
You have gotten some very good answers about why you see a light beam changes direction, or refracts, when hitting a medium with a different refractive index.
In your question, you use the word diverge. I'm not certain how you are using that word. But perhaps you want to know why the light beam changes size. Why light spreads out into a larger width, divergence, then the answer to that question adds in another aspect of refraction.
As you can read from the earlier responses, the reason that light refracts, or changes course, is a function of it's wavelength. A light beam is composed of many different wavelengths, depending upon it's source. Each wavelength diffracts slightly differently.
This will give you the change in the "size" of the light beam.
dj
On Wed, 6 Dec 2006, vthawfeek-at-yahoo.co.in wrote:
} --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both vthawfeek-at-yahoo.co.in as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: vthawfeek-at-yahoo.co.in } Name: V.Thawfeek Mohammed } } Organization: psg tech coll. of engg. } } Title-Subject: [Filtered] optical physics } } Question: why does a light beam diverge when it strikes a medium with } greater refractive index than the medium ,in which it is. }
==============================Original Headers============================== 9, 21 -- From "dljones-at-bestweb.net"-at-bestweb.net Wed Dec 6 12:01:52 2006 9, 21 -- Received: from mta2.srv.hcvlny.cv.net (mta2.srv.hcvlny.cv.net [167.206.4.197]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB6I1qEn017043 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 6 Dec 2006 12:01:52 -0600 9, 21 -- Received: from localhost (ool-44c62883.dyn.optonline.net [68.198.40.131]) 9, 21 -- by mta2.srv.hcvlny.cv.net 9, 21 -- (Sun Java System Messaging Server 6.2-6.01 (built Apr 3 2006)) 9, 21 -- with ESMTP id {0J9V007LC6310XD0-at-mta2.srv.hcvlny.cv.net} for 9, 21 -- microscopy-at-microscopy.com; Wed, 06 Dec 2006 13:01:51 -0500 (EST) 9, 21 -- Date: Wed, 06 Dec 2006 12:50:06 -0500 (Eastern Standard Time) 9, 21 -- From: "David L. Jones" {"dljones-at-bestweb.net"-at-bestweb.net} 9, 21 -- Subject: Re: [Microscopy] viaWWW: optical physics 9, 21 -- In-reply-to: {200612061421.kB6EL8M9015801-at-ns.microscopy.com} 9, 21 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 9, 21 -- To: vthawfeek-at-yahoo.co.in 9, 21 -- Cc: dljones-at-bestweb.net, microscopy-at-microscopy.com 9, 21 -- Message-id: {Pine.WNT.4.64.0612061235110.3120-at-dljtoshiba} 9, 21 -- MIME-version: 1.0 9, 21 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 9, 21 -- Content-transfer-encoding: 7BIT 9, 21 -- References: {200612061421.kB6EL8M9015801-at-ns.microscopy.com} ==============================End of - Headers==============================
This is a really fine answer. I would add one more analogy, since the question (I think) relates to the change in direction of the path of the light beam. In my classes, I refer to the image of a column of a marching band going from a concrete surface to a broken field. If they are exactly perpendicular to the boundary, they continue to go straight, although with some stumbles. If they enter at an angle, then those members who are on the "acute" side of the column slow down first. This "pulls the others along as well, bit by bit, and the overall direction of the march changes.
Date sent: Wed, 6 Dec 2006 11:21:25 -0600 To: jbs-at-temple.edu X-from: bfoster-at-mme1.com Send reply to: bfoster-at-mme1.com
Greetings, These and other wonderful answers to the Snell law question have all been wave-based. Is there an equivalent photon-based answer? Presumably, if you fired one photon at a time at an oblique surface you would get the most counts at the Snell angle; but, with single photons, how are we to understand lines of coherent marchers or even two feet on roller blades? Is this light being weird?
Just wondering... Tobias
} This is a really fine answer. I would add one more analogy, since } the question (I think) relates to the change in direction of the path } of the light beam. In my classes, I refer to the image of a column } of a marching band going from a concrete surface to a broken field. } If they are exactly perpendicular to the boundary, they continue to } go straight, although with some stumbles. If they enter at an angle, } then those members who are on the "acute" side of the column slow } down first. This "pulls the others along as well, bit by bit, and } the overall direction of the march changes. } } Date sent: Wed, 6 Dec 2006 11:21:25 -0600 } To: jbs-at-temple.edu } X-from: bfoster-at-mme1.com } Send reply to: bfoster-at-mme1.com } Subject: [Microscopy] Re: viaWWW: optical physics } } } } } } } } } } } } Hi, } } } } The answer is really quite simple: Snell's Law. The bending of } } light as it crosses the boundary from one RI to another is governed } } by the Laws of Refraction. } } } } The bending discussed in your question requires that several } } conditions be met: First, that there be different RIs on each side } } of the boundary. Second, that the light approach the boundary at } } an angle. } } } } To understand what happens, it is first necessary to understand } } that refractive index is actually a measure of the impact of } } interaction between the electric field of light and the electric } } field of matter. The greater the interaction, the more slowly } } light will travel through that material and the higher the } } refractive index. For instance: } } RI = velocity of light in air/velocity of light in material } } RI of air = 1, velocity of light = 300,000 km/s } } RI of water is 1.33, velocity of light is 225,000 km/s } } RI of immersion oil, glass, and many polymers is approximately } } 1.5, velocity of light = 200,000 km/sec. } } } } As for refraction, the analogy which is often used is that of a } } person roller skating (or in this day and age, roller blading) from } } one surface to another. For example, from the concrete sidewalk } } onto grass. The smooth surface of the concrete is analogous to a } } material with low refractive index; the rougher surface of glass is } } analogous to a material with higher RI. If the skater approaches } } the sidewalk:grass boundary with both feet parallel, both feet will } } slow down by the same amount and the skater will continue skating } } in the same direction. However, if the skater approaches the } } boundary at an angle, one foot will slow down while the other } } remains at the original speed. That different in speed causes the } } skater to pivot toward the material of higher RI. } } } } If you think of light in terms of a wave front rather than a } } simple ray, the analogy transfers easily. If the wave approaches a } } boundary at an angle, the edge of the front which hits the higher } } RI first will slow down, causing the whole wave front to pivot } } around that point. That's what happens, for example, when light } } travels from air (RI = 1.00) into a glass coverslip (RI ~1.5) or a } } droplet of water or a cell (RI ~1.33). You can use the optical } } axis of the microscope as a reference. In these cases, the light } } will bend in TOWARD the optical axis. } } Conversely, when light emerges from a glass slide or a droplet of } } water it will bend AWAY from the OA. Actually this is why we use } } oil immersion: to cause those rays which would normally bend away } } from the OA, causing a loss of both intensity and the critical } } contribution to resolution and edge definition, to bend back toward } } the OA, where they have an opportunity to be captured by the } } objective and make a positive contribution to better imaging. } } } } All of this is explained in detail, with diagrams in Optimizing } } Light Microscopy. If you are interested in a copy, please contact } } Ken Piel here in the MME office for purchasing details (see below). } } } } Hope this was helpful. } } } } Best regards, } } Barbara Foster } } } } Microscopy/Microscopy Education } } 313 S Jupiter Rd, Suite 100 } } Allen, TX 75002 } } P: 972-954-8011 } } W: www.MicroscopyEducation.com } } } } } } MME is now scheduling customized, on-site courses through next } } April. Call us today for details. } } } } P. S. } } Need a good general reference or light microscopy text for the } } Spring semester? Call us today to learn more about "Optimizing } } LIght Microscopy". Copies still available through MME... even for } } class-room lots ... and we give quantity discounts. Call Ken Piel } } at (972)954-8011 or email him at kenpiel-at-mme1.com } } } } } } } } At 08:24 AM 12/6/2006, vthawfeek-at-yahoo.co.in wrote: } } } } } } } } } } } } } This Question/Comment was submitted to the Microscopy Listserver } } } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } } } --------------------------------------------------------------------------- } } } Remember this posting is most likely not from a Subscriber, so } } when replying } } } please copy both vthawfeek-at-yahoo.co.in as well as the } } MIcroscopy Listserver } } } --------------------------------------------------------------------------- } } } } } } Email: vthawfeek-at-yahoo.co.in } } } Name: V.Thawfeek Mohammed } } } } } } Organization: psg tech coll. of engg. } } } } } } Title-Subject: [Filtered] optical physics } } } } } } Question: why does a light beam diverge when it strikes a medium } } with greater refractive index than the medium ,in which it is. } } } } } } --------------------------------------------------------------------------- } } }
On Dec 6, 2006, at 12:25 PM, baskin-at-bio.umass.edu wrote:
} These and other wonderful answers to the Snell law question } have all been wave-based. Is there an equivalent photon-based answer? } Presumably, if you fired one photon at a time at an oblique surface } you would get the most counts at the Snell angle; but, with single } photons, how are we to understand lines of coherent marchers or even } two feet on roller blades? Is this light being weird? } Dear Tobias, The way to look at a single photon is that it consists of a wave packet with a distribution of wavelengths, so that it is pretty much localized and has a reasonably defined momentum, but neither is exact. Then each wave that makes up the wave packet scatters independently resulting in a set of waves distributed around the Snell angle. These waves combine to give you the single photon in the second medium--remember, waves obey the law of superposition; the amplitudes add with the appropriate phases. There is an excellent analogy that you can demonstrate in your scope. If you are examining a crystal in diffraction mode with a beam current so low that only one electron is coming down the column at any time--calculations will convince you that this is achievable with small C2 aperture and high spot size in most scopes--the electron will scatter off the crystal and land on the detector, where it will register in one pixel (approximately, since the electron can scatter again and activate nearby pixels also). Thus, a simple ED experiment has the electron being produced at the gun as a particle, scattering off the specimen as a wave, then activating the detector as a particle again, a clear example of the wave-particle duality. Unless you are very comfortable with quantum mechanics, this is, indeed, weird. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Wed Dec 6 19:52:18 2006 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB71qHht031639 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 6 Dec 2006 19:52:18 -0600 4, 22 -- Received: from water-dog.caltech.edu (water-dog [192.168.1.26]) 4, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 565DD2EFC1 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 6 Dec 2006 17:52:13 -0800 (PST) 4, 22 -- Received: from [192.168.159.233] (jpix-01.caltech.edu [131.215.2.133]) 4, 22 -- by wood-ox.its.caltech.edu (Postfix) with ESMTP id 2F5092EEDB 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 6 Dec 2006 17:52:12 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v624) 4, 22 -- In-Reply-To: {200612062025.kB6KPbI8012001-at-ns.microscopy.com} 4, 22 -- References: {200612062025.kB6KPbI8012001-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {4edc36d2e3c337ee9d65283d4b16b9b3-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] optical physics:Challenge 4, 22 -- Date: Wed, 6 Dec 2006 17:58:16 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.624) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.3.3 ==============================End of - Headers==============================
I have 2 "blöde" questions, not directly related with microscopy, but perhaps one of you can still answer them:
1) I stored LM cryo-sections (unfixed, 5 µm thick) at -28°C for approx. 5 months and now I test the activity of alkaline phosphatase (very positive at the moment of cutting) I see no signal. Control show very strong signal, so it does not come from the technique. I wonder how I lost the enzyme activity. I know for proteins it is better to store à -80°C but I wouldn't expect to lose all the activity! The freezer is a "frost-free" freezer. Does someone know how these freezers work? Could the mechanism of frost-free freezers be detrimental to the enzymatic activity?
2) For the cell line OE21 in the ECACC catalog, I see "Hazard: CX". No other information! I couldn't find this cell line in the ATCC catalog and I couldn't find an explanation on the internet. What does it mean, CX?
About the cleaning of the turbo pump, Pfeiffer indicate for their pumps to soak them in Freon 113 or trichloréthylène, the high-vac side first, as deep as you reach the painted part of the boddy (that mean only the stainles steel part). By that way one can rince the rotor and stator of the pump. I've done this a lot of time, and at the first soak, the solvant become more or less yellow. You change then the solvent until it stays uncolored. Than you can blow hot air through the rouging port, or close the pump with an flange, and pump on it with a roughing pump, to evaporate the residue of solvent.
I suppose one can do that with all turbo pumps, at the condion that the bearing are mounted on both side of the motor. That the case in all with non-magnetic bearing. Pfeiffer's pumps have a magnetic bearing at the high-vac side of the rotor, and a ball bearing at the other end of the motor. Of coarse, one must choose a solvent which disolve the contaminent, and not the viton gasket which protect the bearing.
I had until now much success with that way, seeing a real difference in vaccum performence, as well in total pressure as in pollution measurement with a RGA.
Of coarse, for a complet cleaning, with a heavy pollution, on must desmantle the pump. That's an other stuff ! But it's worth to try the soaking methode, before sending the pump back to the manufacturer.
Hope it's help
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
john.mardinly-at-intel.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Scott; } Thanks to you and everyone that responded with their thoughts on } this problem. The feeling among our group now is that your thought that } outgassing from the grease in the mechanical part of the diaphragm pumps } can backstream when the diaphragms fail is the most likely diagnosis. } This particular pump had a huge tear in the failed diaphragm, but this } was a bit of a surprise, since our many Gatan PIPS have exactly the same } pumps, and we routinely run them until diaphragm failure, although they } tend to fail with smaller tears, and perhaps the gas load from the argon } ion guns tends to keep them cleaner by purging backstreaming. } We have cleaned the vacuum chamber with solvents (and it was a } mess), but cleaning the turbo pump will be difficult, since it cannot be } disassembled. A nitrogen purge will probably be the best method of } cleaning the turbo. We also now intend to proactively change all of our } diaphragms on an annual basis, rather than running them to failure, in } the hope that this sort of mess never recurs. } } John Mardinly } Intel Corporation } } Disclaimer: This is a communication from this author and does not } represent } an opinion of Intel Corporation. } } -----Original Message----- } X-from: Scott Walck [mailto:walck-at-southbaytech.com] } Sent: Friday, December 01, 2006 2:43 PM } To: Microscopy-at-microscopy.com } Cc: Mardinly, John } Subject: RE: [Microscopy] Contamination of Vacuum Specimen Storage } System } } John, } I think that the sulfur came from the rubber diaphragms. I'm surprised, } because I thought that they would have been VitonR. Either that or } lubrication oil from moving parts that came through the failed diaphragm } and } was exposed to the system. Was the diaphragm torn or had holes in it? } Sulfur's atomic weight just might let it get through a turbo pump. } Anyway, } I think what you might be able to do is "getter" the sulfur in your } system. } Get some copper powder and put it in your system for a while. The } smaller } the powder particles and the more open the container, the better } gettering } that you will have. Silver ought to work also. It will take some time } to } getter the sulfur in your system out, but given some time, it should be } able } to do it. You will also be able to monitor the progress on the copper } particles if you periodically change the copper. } } In the mean time, you might want to consider storing your samples with } our } SampleSaverT system. We have a specially vented TEM storage box for the } SampleSaverT container system that will take TEM grids. If the samples } are } FIB cuts, you can store them in the SampleSaverT container in our } FortressT } FIB holders that will also fit into the SampleSaverT container. The } SampleSaverT containers worked for my cross section samples of Low-E } glass } where there are two layers of Ag exposed. Unfortunately, I haven't } gotten } any feedback yet from how well they work for copper metallization } samples. } I expect them to work as well there, but as I said, I don't have hard } evidence, yet. } } BTW, Generally, the suggested maintenance replacement time on diaphragms } for } pumps is about a year. } } Disclaimer: South Bay Technology, Inc. makes and sells the SampleSaverT } container and FortressT FIB holders. } } } -Scott } } Scott D. Walck, Ph.D. } Technical Director } South Bay Technology, Inc. } 1120 Via Callejon } San Clemente, CA 92673 } } US Toll Free: 1-800-728-2233 } Tel: (949) 492-2600 } Fax: (949) 492-1499 } } www.southbaytech.com } walck-at-southbaytech.com } } -----Original Message----- } X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com] } Sent: Friday, December 01, 2006 11:40 AM } To: Walck-at-SouthBayTech.com } Subject: [Microscopy] Contamination of Vacuum Specimen Storage System } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } } } We have a Gatan Model 655 Dry Pumping Station } (www.gatan.com/holders/655_drypumpingstation.html) equipped with pods } for } storage of TEM specimens. In particular, TEM cross-sections of } integrated } circuits using copper interconnects seem to be particularly vulnerable } to } corrosion in atmosphere. This vacuum station has been essential in } protecting the specimens that we put so much work into making, and has } been } very successful for quite some time. The dry vacuum system consists of a } 'drag' pump, which is essentially a turbo pump, backed by a diaphragm } type } rough pump. Last summer, one of the rubber diaphragms failed in the } backing } pump, and the system ran for a few days with degraded vacuum. After the } diaphragms were replaced and the high vacuum restored to the E-5 Torr } range, } we found that TEM cross-sections of integrated circuits using copper } interconnects were destroyed in just a few hours if stored in this } vacuum } system. EDX of the copper corrosion product showed the presence of } sulfur. } We have tried cleaning the storage pods, and part of the inside of the } vacuum chamber, but have been totally frustrated in being able to } restore } the essential function of this vacuum storage system. Any suggestions as } to } what may have happened, and what we can do to clean up the system would } be } very much appreciated. Thank you. } } John Mardinly } Intel Corporation } } Disclaimer: This is a communication from this author and does not } represent } an opinion of Intel Corporation. } } } } ==============================Original } Headers============================== } 6, 30 -- From john.mardinly-at-intel.com Fri Dec 1 13:35:49 2006 6, 30 -- } Received: from mga01.intel.com (mga01.intel.com [192.55.52.88]) } 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id } kB1JZnHb024448 } 6, 30 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 1 Dec 2006 } 13:35:49 -0600 } 6, 30 -- Received: from fmsmga001.fm.intel.com ([10.253.24.23]) } 6, 30 -- by mga01.intel.com with ESMTP; 01 Dec 2006 11:35:32 -0800 } 6, 30 -- Received: from scsmsx332.sc.intel.com (HELO } scsmsx332.amr.corp.intel.com) ([10.3.90.6]) } 6, 30 -- by fmsmga001.fm.intel.com with ESMTP; 01 Dec 2006 11:35:32 } -0800 } 6, 30 -- X-ExtLoop1: 1 } 6, 30 -- X-IronPort-AV: i="4.09,486,1157353200"; } 6, 30 -- d="scan'208"; a="171762165:sNHT19309206" } 6, 30 -- Received: from scsmsx413.amr.corp.intel.com ([10.3.90.32]) by } scsmsx332.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); } 6, 30 -- Fri, 1 Dec 2006 11:35:31 -0800 } 6, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 30 -- } Content-class: urn:content-classes:message 6, 30 -- MIME-Version: 1.0 6, } 30 } -- Content-Type: text/plain; } 6, 30 -- charset="us-ascii" } 6, 30 -- Subject: Contamination of Vacuum Specimen Storage System 6, 30 } -- } Date: Fri, 1 Dec 2006 11:35:31 -0800 6, 30 -- Message-ID: } {1DF4C4D62339DB4C9C98DF04213995B601F0A84F-at-scsmsx413.amr.corp.intel.com} } 6, 30 -- X-MS-Has-Attach: } 6, 30 -- X-MS-TNEF-Correlator: } 6, 30 -- Thread-Topic: Contamination of Vacuum Specimen Storage System } 6, } 30 -- Thread-Index: } AccVcgLpZR8yy6qqRpi55yhxXPXijQAAIjDgAAAaArAAAVCSIAAB3rtQ } 6, 30 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 6, 30 -- To: } {Microscopy-at-msa.microscopy.com} 6, 30 -- X-OriginalArrivalTime: 01 Dec } 2006 } 19:35:31.0926 (UTC) FILETIME=[DD2F8F60:01C7157F] 6, 30 -- } Content-Transfer-Encoding: 8bit 6, 30 -- X-MIME-Autoconverted: from } quoted-printable to 8bit by ns.microscopy.com id kB1JZnHb024448 } ==============================End of - } Headers============================== } } } ==============================Original Headers============================== } 26, 30 -- From john.mardinly-at-intel.com Wed Dec 6 10:56:04 2006 } 26, 30 -- Received: from mga02.intel.com (mga02.intel.com [134.134.136.20]) } 26, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB6Gu3dq024503 } 26, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Dec 2006 10:56:03 -0600 } 26, 30 -- Received: from orsmga001.jf.intel.com ([10.7.209.18]) } 26, 30 -- by mga02.intel.com with ESMTP; 06 Dec 2006 08:51:57 -0800 } 26, 30 -- Received: from scsmsx331.sc.intel.com (HELO scsmsx331.amr.corp.intel.com) ([10.3.90.4]) } 26, 30 -- by orsmga001.jf.intel.com with ESMTP; 06 Dec 2006 08:51:55 -0800 } 26, 30 -- X-ExtLoop1: 1 } 26, 30 -- X-IronPort-AV: i="4.09,505,1157353200"; } 26, 30 -- d="scan'208"; a="171184232:sNHT45583097" } 26, 30 -- Received: from scsmsx413.amr.corp.intel.com ([10.3.90.32]) by scsmsx331.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); } 26, 30 -- Wed, 6 Dec 2006 08:51:52 -0800 } 26, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 26, 30 -- Content-class: urn:content-classes:message } 26, 30 -- MIME-Version: 1.0 } 26, 30 -- Content-Type: text/plain; } 26, 30 -- charset="us-ascii" } 26, 30 -- Subject: RE: [Microscopy] Contamination of Vacuum Specimen Storage System } 26, 30 -- Date: Wed, 6 Dec 2006 08:51:52 -0800 } 26, 30 -- Message-ID: {1DF4C4D62339DB4C9C98DF04213995B601FBA503-at-scsmsx413.amr.corp.intel.com} } 26, 30 -- X-MS-Has-Attach: } 26, 30 -- X-MS-TNEF-Correlator: } 26, 30 -- Thread-Topic: [Microscopy] Contamination of Vacuum Specimen Storage System } 26, 30 -- Thread-Index: AccVgIJVL6E6+CDvTlWJF2VEgMf9YAAE2UawAPA2/rA= } 26, 30 -- From: "Mardinly, John" {john.mardinly-at-intel.com} } 26, 30 -- To: "Scott Walck" {walck-at-southbaytech.com} , {Microscopy-at-microscopy.com} } 26, 30 -- X-OriginalArrivalTime: 06 Dec 2006 16:51:52.0884 (UTC) FILETIME=[D4A56740:01C71956] } 26, 30 -- Content-Transfer-Encoding: 8bit } 26, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kB6Gu3dq024503 } ==============================End of - Headers============================== }
==============================Original Headers============================== 12, 30 -- From jacques.faerber-at-ipcms.u-strasbg.fr Thu Dec 7 02:39:36 2006 12, 30 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.157]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB78dZ4e031374 12, 30 -- for {microscopy-at-microscopy.com} ; Thu, 7 Dec 2006 02:39:36 -0600 12, 30 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 12, 30 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id kB78dWTS099809 12, 30 -- for {microscopy-at-microscopy.com} ; Thu, 7 Dec 2006 09:39:32 +0100 (CET) 12, 30 -- Received: from [130.79.54.3] (odhinn.u-strasbg.fr [130.79.54.3]) 12, 30 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 7F4873EC114 12, 30 -- for {Microscopy-at-Microscopy.Com} ; Thu, 7 Dec 2006 09:38:48 +0100 (CET) 12, 30 -- Message-ID: {4577D32D.9040408-at-ipcms.u-strasbg.fr} 12, 30 -- Date: Thu, 07 Dec 2006 09:39:09 +0100 12, 30 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 12, 30 -- User-Agent: Thunderbird 1.5.0.8 (X11/20061117) 12, 30 -- MIME-Version: 1.0 12, 30 -- To: Microscopy-at-microscopy.com 12, 30 -- Subject: Re: [Microscopy] RE: Contamination of Vacuum Specimen Storage System 12, 30 -- + turbo pump 12, 30 -- References: {200612061703.kB6H3tTP011952-at-ns.microscopy.com} 12, 30 -- In-Reply-To: {200612061703.kB6H3tTP011952-at-ns.microscopy.com} 12, 30 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 30 -- Content-Transfer-Encoding: 8bit 12, 30 -- X-IPCMS-MailScanner: Found to be clean 12, 30 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 12, 30 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-2.0.2 (mailhost.u-strasbg.fr [130.79.200.157]); Thu, 07 Dec 2006 09:39:32 +0100 (CET) 12, 30 -- X-Virus-Scanned: ClamAV 0.88.5/2299/Thu Dec 7 08:36:50 2006 on mr7.u-strasbg.fr 12, 30 -- X-Virus-Status: Clean 12, 30 -- X-Spam-Status: No, score=-0.5 required=5.0 tests=AWL autolearn=disabled 12, 30 -- version=3.1.7 12, 30 -- X-Spam-Checker-Version: SpamAssassin 3.1.7 (2006-10-05) on mr7.u-strasbg.fr ==============================End of - Headers==============================
actually Newton came up with a particle-theory of refraction.
The photons are accelerated (Don't ask me how) at the surface from lower refractive index to higher. This acceleration is normal to the surface and leaves velocity-components parallel to the surface unaffected. Thus the velocity-vector changes direction towards the normal.
Notice that this theory requires higher velocity in the high-index medium, whereas the wave-expalnation requires lower velocity. The answer to this puzzle is that the wave-explanation uses the phase-velocity whereas, speaking in wave-terms, Newton would have been talking about the group-velocity, which Bill Tivol refers to in his mail. In fact in a dispersive medium (different colours have different phase-velocities) the group- and phase-velocities are different. The group-velocity can actually be larger, in support of Newton.
Philip
-----Original Message----- X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] Sent: 06 December 2006 21:28 To: Philip.Koeck-at-biosci.ki.se
Mohammed,
some clarification would be helpful. Do you really mean "diverge" or do you mean "split into different colors"?
An already divergent monochromatic beam should actually diverge less after passing into the higher refractive index.
Philip
-----Original Message----- X-from: dljones-at-bestweb.net [mailto:dljones-at-bestweb.net] Sent: 06 December 2006 19:04 To: Philip.Koeck-at-biosci.ki.se
This thread is following the route to a Nobel prize in Physics.. have a read of Richard Feynman's "QED-the strange theory of light and matter", which addresses exactly this point and is a summary of the work for which he was awarded his gong. I haven't read it for a few years, but I remember him starting with glass being 80% transmissive, and asking how could individual photons 'know' whether to cross the glass or not to give the right answer of 80%. Like most of his stuff it is very readable and entertaining. And yes, the world is actually weirder than you think it is..
-----Original Message----- X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] Sent: 06 December 2006 20:27 To: Richard Beanland
Greetings, These and other wonderful answers to the Snell law question have all been wave-based. Is there an equivalent photon-based answer? Presumably, if you fired one photon at a time at an oblique surface you would get the most counts at the Snell angle; but, with single photons, how are we to understand lines of coherent marchers or even two feet on roller blades? Is this light being weird?
Just wondering... Tobias
} This is a really fine answer. I would add one more analogy, since } the question (I think) relates to the change in direction of the path } of the light beam. In my classes, I refer to the image of a column } of a marching band going from a concrete surface to a broken field. } If they are exactly perpendicular to the boundary, they continue to } go straight, although with some stumbles. If they enter at an angle, } then those members who are on the "acute" side of the column slow } down first. This "pulls the others along as well, bit by bit, and } the overall direction of the march changes. } } Date sent: Wed, 6 Dec 2006 11:21:25 -0600 } To: jbs-at-temple.edu } X-from: bfoster-at-mme1.com } Send reply to: bfoster-at-mme1.com } Subject: [Microscopy] Re: viaWWW: optical physics } } } } } } } } } } } } Hi, } } } } The answer is really quite simple: Snell's Law. The bending of } } light as it crosses the boundary from one RI to another is governed } } by the Laws of Refraction. } } } } The bending discussed in your question requires that several } } conditions be met: First, that there be different RIs on each side } } of the boundary. Second, that the light approach the boundary at } } an angle. } } } } To understand what happens, it is first necessary to understand } } that refractive index is actually a measure of the impact of } } interaction between the electric field of light and the electric } } field of matter. The greater the interaction, the more slowly } } light will travel through that material and the higher the } } refractive index. For instance: } } RI = velocity of light in air/velocity of light in material } } RI of air = 1, velocity of light = 300,000 km/s } } RI of water is 1.33, velocity of light is 225,000 km/s } } RI of immersion oil, glass, and many polymers is approximately } } 1.5, velocity of light = 200,000 km/sec. } } } } As for refraction, the analogy which is often used is that of a } } person roller skating (or in this day and age, roller blading) from } } one surface to another. For example, from the concrete sidewalk } } onto grass. The smooth surface of the concrete is analogous to a } } material with low refractive index; the rougher surface of glass is } } analogous to a material with higher RI. If the skater approaches } } the sidewalk:grass boundary with both feet parallel, both feet will } } slow down by the same amount and the skater will continue skating } } in the same direction. However, if the skater approaches the } } boundary at an angle, one foot will slow down while the other } } remains at the original speed. That different in speed causes the } } skater to pivot toward the material of higher RI. } } } } If you think of light in terms of a wave front rather than a } } simple ray, the analogy transfers easily. If the wave approaches a } } boundary at an angle, the edge of the front which hits the higher } } RI first will slow down, causing the whole wave front to pivot } } around that point. That's what happens, for example, when light } } travels from air (RI = 1.00) into a glass coverslip (RI ~1.5) or a } } droplet of water or a cell (RI ~1.33). You can use the optical } } axis of the microscope as a reference. In these cases, the light } } will bend in TOWARD the optical axis. } } Conversely, when light emerges from a glass slide or a droplet of } } water it will bend AWAY from the OA. Actually this is why we use } } oil immersion: to cause those rays which would normally bend away } } from the OA, causing a loss of both intensity and the critical } } contribution to resolution and edge definition, to bend back toward } } the OA, where they have an opportunity to be captured by the } } objective and make a positive contribution to better imaging. } } } } All of this is explained in detail, with diagrams in Optimizing } } Light Microscopy. If you are interested in a copy, please contact } } Ken Piel here in the MME office for purchasing details (see below). } } } } Hope this was helpful. } } } } Best regards, } } Barbara Foster } } } } Microscopy/Microscopy Education } } 313 S Jupiter Rd, Suite 100 } } Allen, TX 75002 } } P: 972-954-8011 } } W: www.MicroscopyEducation.com } } } } } } MME is now scheduling customized, on-site courses through next } } April. Call us today for details. } } } } P. S. } } Need a good general reference or light microscopy text for the } } Spring semester? Call us today to learn more about "Optimizing } } LIght Microscopy". Copies still available through MME... even for } } class-room lots ... and we give quantity discounts. Call Ken Piel } } at (972)954-8011 or email him at kenpiel-at-mme1.com } } } } } } } } At 08:24 AM 12/6/2006, vthawfeek-at-yahoo.co.in wrote: } } } } } } } } } } } } } This Question/Comment was submitted to the Microscopy Listserver } } } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } } } ----------------------------------------------------------------------- ---- } } } Remember this posting is most likely not from a Subscriber, so } } when replying } } } please copy both vthawfeek-at-yahoo.co.in as well as the } } MIcroscopy Listserver } } } ----------------------------------------------------------------------- ---- } } } } } } Email: vthawfeek-at-yahoo.co.in } } } Name: V.Thawfeek Mohammed } } } } } } Organization: psg tech coll. of engg. } } } } } } Title-Subject: [Filtered] optical physics } } } } } } Question: why does a light beam diverge when it strikes a medium } } with greater refractive index than the medium ,in which it is. } } } } } } ----------------------------------------------------------------------- ---- } } }
==============================Original Headers============================== 6, 19 -- From baskin-at-bio.umass.edu Wed Dec 6 14:25:31 2006 6, 19 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB6KPVfD011840 6, 19 -- for {microscopy-at-microscopy.com} ; Wed, 6 Dec 2006 14:25:31 -0600 6, 19 -- Received: from [172.30.55.79] (eutopia [128.119.55.30]) 6, 19 -- by marlin.bio.umass.edu (8.13.6/8.13.6) with ESMTP id kB6KPQZB025363; 6, 19 -- Wed, 6 Dec 2006 15:25:27 -0500 (EST) 6, 19 -- Mime-Version: 1.0 6, 19 -- Message-Id: {p0623090dc19cd66dc686-at-[172.30.55.79]} 6, 19 -- In-Reply-To: {200612062000.kB6K0oST001676-at-ns.microscopy.com} 6, 19 -- References: {200612062000.kB6K0oST001676-at-ns.microscopy.com} 6, 19 -- Date: Wed, 6 Dec 2006 15:25:24 -0500 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 6, 19 -- Subject: optical physics:Challenge 6, 19 -- Cc: jbs-at-temple.edu 6, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 6, 19 -- X-Greylist: Sender succeded SMTP AUTH authentication, not delayed by milter-greylist-1.6 (marlin.bio.umass.edu [128.119.55.19]); Wed, 06 Dec 2006 15:25:29 -0500 (EST) 6, 19 -- X-Scanned-By: MIMEDefang 2.54 on 128.119.55.19 ==============================End of - Headers==============================
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==============================Original Headers============================== 15, 32 -- From richard.beanland-at-bookham.com Thu Dec 7 03:45:10 2006 15, 32 -- Received: from mail83.messagelabs.com (mail83.messagelabs.com [195.245.231.83]) 15, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kB79j90N032413 15, 32 -- for {microscopy-at-microscopy.com} ; Thu, 7 Dec 2006 03:45:10 -0600 15, 32 -- X-VirusChecked: Checked 15, 32 -- X-Env-Sender: richard.beanland-at-bookham.com 15, 32 -- X-Msg-Ref: server-15.tower-83.messagelabs.com!1165484708!42046564!1 15, 32 -- X-StarScan-Version: 5.5.10.7; banners=bookham.com,-,- 15, 32 -- X-Originating-IP: [213.249.209.179] 15, 32 -- Received: (qmail 31146 invoked from network); 7 Dec 2006 09:45:08 -0000 15, 32 -- Received: from unknown (HELO cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 15, 32 -- by server-15.tower-83.messagelabs.com with SMTP; 7 Dec 2006 09:45:08 -0000 15, 32 -- Received: from cas-smx-02.BOOKHAM.ENTERPRISE.PRI ([10.4.0.223]) by cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI with Microsoft SMTPSVC(6.0.3790.1830); 15, 32 -- Thu, 7 Dec 2006 09:45:15 +0000 15, 32 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 15, 32 -- Content-class: urn:content-classes:message 15, 32 -- MIME-Version: 1.0 15, 32 -- Content-Type: text/plain; 15, 32 -- charset="us-ascii" 15, 32 -- Subject: RE: [Microscopy] optical physics:Challenge 15, 32 -- Date: Thu, 7 Dec 2006 09:45:14 -0000 15, 32 -- Message-ID: {9645D3E33E4C6548B12A7B25F611533E2CC54D-at-cas-smx-02.BOOKHAM.ENTERPRISE.PRI} 15, 32 -- X-MS-Has-Attach: 15, 32 -- X-MS-TNEF-Correlator: 15, 32 -- Thread-Topic: [Microscopy] optical physics:Challenge 15, 32 -- thread-index: AccZdN5xFl4Td6sqTl6++eu/sEqL/AAbkU2w 15, 32 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 15, 32 -- To: {baskin-at-bio.umass.edu} 15, 32 -- Cc: {microscopy-at-microscopy.com} 15, 32 -- X-OriginalArrivalTime: 07 Dec 2006 09:45:15.0631 (UTC) FILETIME=[65E84FF0:01C719E4] 15, 32 -- Content-Transfer-Encoding: 8bit 15, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kB79j90N032413 ==============================End of - Headers==============================
Hello Stephane, The frost-free freezer may have been the culprit. They keep and average temp of -20, but the temp. cycles so that the frost can be cleared. I have one in my lab, too, but I keep anything that may be really temp. sensitive in a separate -20 that is definitely NOT frost free (we just spent 2 days defrosting it!). On the other hand, I have also kept cryo sections of chicken heart in the frost-free -20 for months and continued to have good immuno labelling. I think perhaps that the enzymes are more sensitve to freeze-thaw. Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175 http://www.cornellcelldevbiology.org http://www.cornellbiochem.org
==============================Original Headers============================== 1, 24 -- From lcgould-at-med.cornell.edu Thu Dec 7 08:42:56 2006 1, 24 -- Received: from smtp-gw1.med.cornell.edu (smtp-gw1.med.cornell.edu [140.251.3.74]) 1, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB7Egufc018796 1, 24 -- for {microscopy-at-microscopy.com} ; Thu, 7 Dec 2006 08:42:56 -0600 1, 24 -- Received: from mpx3.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) 1, 24 -- by smtp-gw1.med.cornell.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id kB7EgdjE011089; 1, 24 -- Thu, 7 Dec 2006 09:42:53 -0500 (EST) 1, 24 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23]) 1, 24 -- by mpx3.med.cornell.edu 1, 24 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 1, 24 -- with ESMTPA id {0J9W00GKVRIY1610-at-mpx3.med.cornell.edu} ; Thu, 1, 24 -- 07 Dec 2006 09:42:35 -0500 (EST) 1, 24 -- Date: Thu, 07 Dec 2006 09:42:32 -0500 1, 24 -- From: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu} 1, 24 -- Subject: Re: [Microscopy] 2 questions not directly related to microscopy 1, 24 -- In-reply-to: {200612070754.kB77sR43020952-at-ns.microscopy.com} 1, 24 -- Sender: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu} 1, 24 -- To: nizets2-at-yahoo.com, Microscopy Listserver {microscopy-at-microscopy.com} 1, 24 -- Message-id: {p06230905c19dd7efca5e-at-[140.251.48.23]} 1, 24 -- MIME-version: 1.0 1, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed 1, 24 -- Content-transfer-encoding: 7BIT 1, 24 -- References: {200612070754.kB77sR43020952-at-ns.microscopy.com} 1, 24 -- X-PMX-Version: 5.2.2.285561, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2006.12.7.62432 ==============================End of - Headers==============================
Rhonda, We have had our Leica EM Stain unit for about 2 years now. I love it and would recommend it's purchase. Just be aware of a number of things:
1. Leica can only ship bottled 0.5% uranyl stain. I feel my epoxy resins are too weakly stained with this so I modify to 2%. 2. I prefer the 40-grid Hiroaka Plates from Electron Microscopy Sciences rather than the Leica 25-grid plates. The Hiroaka plates seem to be made of a different plastic and maintain their flexibility longer (I discard after about 10 runs). Unfortunately I found the Leica plates start to loose their grip on the grids after a couple of uses. 3. I have set the system to use 15ml of stain per run but you have the option of 5-25ml in 5ml increments. 3. Adherence to a rigorous system cleaning schedule is extremely important for section cleanliness. The system should be stored in an "Extended Wash" cycle using 3% nitric acid. Prior to staining run, boil and cool ~2L dH2O. Finish the extended wash cycle, run and finish a new extended wash cycle, and only THEN run your wash cycle. This can take 1.5 hrs. Now you can stain (~1 hr procedure).
The times may sound long, but you simply walk away and do something else. You can even set it up with a delay function to start early the next morning and finish when just as you're walking in. If you want my system details, give a holler.
Best regards,
Walter F. Bobrowski Sr. Scientist Pfizer Global R&D 2800 Plymouth Rd. Ann Arbor, MI 48105
TEL: 734-622-7814 FAX: 734-622-5718
"The ultimate human freedom is the ability to choose one's attitude in a given set of circumstances." -Viktor Frankl
-----Original Message----- X-from: rra-at-stowers-institute.org [mailto:rra-at-stowers-institute.org] Sent: Tuesday, December 05, 2006 5:33 PM To: Bobrowski, Walter
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/ ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both rra-at-stowers-institute.org as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: rra-at-stowers-institute.org Name: Rhonda Allen
Organization: Stowers Institute
Title-Subject: [Filtered] Leica EM-Stain
Question: Hello, I am interested in purchasing the Leica EM-Stain. Does anyone have any comments, positive and negative, that I should take into consideration before making such an investment? We will be using it on formvar coated slot grids. Thanks in advance, Rhonda Allen BA HT(ASCP)HTL, QIHC Stowers Institute for Medical Research Kansas City, Missouri 816-926-4346
==============================Original Headers============================== 7, 12 -- From zaluzec-at-microscopy.com Tue Dec 5 16:26:17 2006 7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB5MQGaV013340 7, 12 -- for {microscopy-at-microscopy.com} ; Tue, 5 Dec 2006 16:26:17 -0600 7, 12 -- Mime-Version: 1.0 7, 12 -- X-Sender: (Unverified) 7, 12 -- Message-Id: {p06110400c19ba26526ef-at-[206.69.208.22]} 7, 12 -- Date: Tue, 5 Dec 2006 16:26:16 -0600 7, 12 -- To: microscopy-at-microscopy.com 7, 12 -- From: rra-at-stowers-institute.org (by way of MicroscopyListserver) 7, 12 -- Subject: viaWWW: Leica EM-Stain 7, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers============================== ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately.
==============================Original Headers============================== 22, 29 -- From Walter.Bobrowski-at-pfizer.com Thu Dec 7 08:51:14 2006 22, 29 -- Received: from gromsgom01.pfizer.com (gromsgo.pfizer.com [148.168.224.84]) 22, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB7EpE6t001284 22, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Dec 2006 08:51:14 -0600 22, 29 -- Received: from mopamrexc02.amer.pfizer.com (mopamrexc02.pfizer.com [170.116.30.68]) 22, 29 -- by gromsgom01.pfizer.com (8.13.4/8.13.4) with ESMTP id kB7Eol31014318; 22, 29 -- Thu, 7 Dec 2006 09:50:47 -0500 22, 29 -- Received: from mopamrexc01.amer.pfizer.com ([170.116.32.254]) by mopamrexc02.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.1830); 22, 29 -- Thu, 7 Dec 2006 09:51:13 -0500 22, 29 -- Received: from anaamrexm01.amer.pfizer.com ([162.48.101.10]) by mopamrexc01.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.1830); 22, 29 -- Thu, 7 Dec 2006 09:51:13 -0500 22, 29 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5.7226.0 22, 29 -- Content-class: urn:content-classes:message 22, 29 -- MIME-Version: 1.0 22, 29 -- Content-Type: text/plain; 22, 29 -- charset="US-ASCII" 22, 29 -- Subject: RE: [Microscopy] viaWWW: Leica EM-Stain 22, 29 -- Date: Thu, 7 Dec 2006 09:51:11 -0500 22, 29 -- Message-ID: {CF89AF6ABD93B746A55BCCD9480A4B3D082A1338-at-anaamrexm01.amer.pfizer.com} 22, 29 -- X-MS-Has-Attach: 22, 29 -- X-MS-TNEF-Correlator: 22, 29 -- Thread-Topic: [Microscopy] viaWWW: Leica EM-Stain 22, 29 -- Thread-Index: AccYvbSnWhtxHadlRT6pxmbEKRP8QgBReZmw 22, 29 -- From: "Bobrowski, Walter" {Walter.Bobrowski-at-pfizer.com} 22, 29 -- To: {rra-at-stowers-institute.org} , {Microscopy-at-microscopy.com} 22, 29 -- X-OriginalArrivalTime: 07 Dec 2006 14:51:13.0295 (UTC) FILETIME=[23ED89F0:01C71A0F] 22, 29 -- X-Proofpoint-Spam-Reason: safe 22, 29 -- Content-Transfer-Encoding: 8bit 22, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kB7EpE6t001284 ==============================End of - Headers==============================
Owen, Seal the BEEM cap with some nail polish (or whatever) and turn upside down. This will let the long axis of the wood be parallel to the surface. If you want endgrain endon, after polymerization, use a saw of some kind and cut a few mm "slice" off the end and then trim and mount upright on a blank block. Superglue bonds to epoxies really tightly so this works, though admittedly inelegant. You can also buy BEEM style caps (they are in fact TAAB capsules) that have flat bottoms so you don't have to seal the tops and flip.
Hope this helps, Tobias
} } All, } } How would one go about embedding wood? I am having trouble orienting } the end grain in a BEEM capsule. } } Owen Mills } Michigan Tech University }
7-Dec-2006 The Dow Chemical Company has an opening for a Ph.D. Chemist, Polymer Scientist or Materials Scientist in the microscopy discipline of our Global Analytical Sciences Laboratory.
Location of current position: Schkopau, Germany Job Title: Senior Research Chemist Degree: Ph.D. Discipline: Analytical Chemistry, Physical Chemistry, Materials Science, Physics, or Polymer Science
Job Description Overview: An immediate opening exists for a skilled microscopist in Dow's Global Analytical Sciences Laboratory within Dow's Corporate R&D function. We are seeking an individual with a passion for characterization science and a desire to both apply this science to complex industrial problems and to advance the technology. Candidate will be responsible for using microscopy and microanalysis to solve a wide range of materials problems in multiple application areas. The candidate will be expected to work as part of a cross-functional team in partnership with Dow R&D personnel in a variety of technology areas including catalysis, polymer science, and materials science.
For this position we are seeking candidates who are proficient in a variety of microscopy techniques. Working experience in electron microscopy and/or scanned probe microscopy is essential. Additional skills in ultramicrotomy, energy dispersive x-ray analysis, and capability in other microscopy techniques and surface science techniques such as optical, TEM, and XPS is preferred. Candidates must have excellent problem solving skills, a background in polymer science is a plus. Ph.D. in Analytical Chemistry, Physical Chemistry, Physics, Engineering or Materials Science is preferred, but an individual with a Masters degree and relevant work experience will also be considered. The position is located in Schkopau, Germany (Halle/Leipzig area). Fluency in English is required, fluency in German is a plus.
If you are highly qualified and are interested in this job, please submit your electronic resume and curriculum vitae directly to tdominowski-at-dow.com or send paper copy to: Dr. Georg Bar Dow Olefinverbund GmbH PF 1163, 06201 Merseburg, Germany.
Best Regards,
Bill William A. Heeschen, Ph.D. Microscopy, Digital Imaging The Dow Chemical Company Midland, MI 48674 waheeschen-at-dow.com
Dow is a leader in science and technology, providing innovative chemical, plastic and agricultural products and services to many essential consumer markets. With annual sales of $40 billion, Dow serves customers in 175 countries and a wide range of markets that are vital to human progress: food, transportation, health and medicine, personal and home care, and building and construction, among others. Committed to the principles of sustainable development, Dow and its 43,000 employees seek to balance economic, environmental and social responsibilities. References to "Dow" or the "Company" mean The Dow Chemical Company and its consolidated subsidiaries unless otherwise expressly noted.
==============================Original Headers============================== 12, 23 -- From WAHeeschen-at-dow.com Thu Dec 7 09:23:48 2006 12, 23 -- Received: from mail130.messagelabs.com (mail130.messagelabs.com [216.82.250.163]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kB7FNmEG030204 12, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Dec 2006 09:23:48 -0600 12, 23 -- X-VirusChecked: Checked 12, 23 -- X-Env-Sender: WAHeeschen-at-dow.com 12, 23 -- X-Msg-Ref: server-15.tower-130.messagelabs.com!1165505026!4106543!1 12, 23 -- X-StarScan-Version: 5.5.10.7; banners=-,-,- 12, 23 -- X-Originating-IP: [216.99.65.28] 12, 23 -- Received: (qmail 4543 invoked from network); 7 Dec 2006 15:23:47 -0000 12, 23 -- Received: from mail8.dow.com (HELO mante89.nam.dow.com) (216.99.65.28) 12, 23 -- by server-15.tower-130.messagelabs.com with SMTP; 7 Dec 2006 15:23:47 -0000 12, 23 -- Received: by mante89.nam.dow.com with Internet Mail Service (5.5.2658.3) 12, 23 -- id {W5R91SGC} ; Thu, 7 Dec 2006 10:23:46 -0500 12, 23 -- Message-ID: {0F0E6B7B4F6AE84CBC5E7AF57057FBA8084EB2-at-USMDLMDOWX022.dow.com} 12, 23 -- From: "Heeschen, Bill (WA)" {WAHeeschen-at-dow.com} 12, 23 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 12, 23 -- Subject: Position available: The Dow Chemical Company - Analytical Scienc 12, 23 -- es Career Opportunities 12, 23 -- Date: Thu, 7 Dec 2006 10:23:01 -0500 12, 23 -- MIME-Version: 1.0 12, 23 -- X-Mailer: Internet Mail Service (5.5.2658.3) 12, 23 -- Content-Type: text/plain ==============================End of - Headers==============================
Hello Everyone, I find that I need to start shopping for a cryo ultra microtome. Any advise the members of this list would care to share is welcome as would be product literature and price quotes from vendors. We are targeting 2008, but we need to start floating numbers and requirements in front of upper management now.
Please feel free (gasp!) to snail or E-mail me literature.
Thanks!!!
Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333 330-668-2235 Ext. 238 This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you.
==============================Original Headers============================== 19, 17 -- From frank.karl-at-degussa.com Thu Dec 7 14:19:11 2006 19, 17 -- Received: from framailout1.rz.itson.com (mailout2.degussa.com [149.216.91.173]) 19, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB7KJA0U017984 19, 17 -- for {microscopy-at-msa.microscopy.com} ; Thu, 7 Dec 2006 14:19:10 -0600 19, 17 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [10.88.88.98]) 19, 17 -- by framailout1.rz.itson.com (8.13.4/8.13.4/Debian-3sarge3) with SMTP id kB7KJ7dV028928 19, 17 -- for {microscopy-at-msa.microscopy.com} ; Thu, 7 Dec 2006 21:19:08 +0100 19, 17 -- Subject: request for product (microtomes) information and prices 19, 17 -- To: microscopy-at-msa.microscopy.com 19, 17 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 19, 17 -- Message-ID: {OF97E936E0.78C31E6A-ON8625723D.006EDC57-8525723D.006F9BC1-at-degussa.com} 19, 17 -- From: frank.karl-at-degussa.com 19, 17 -- Date: Thu, 7 Dec 2006 15:19:04 -0500 19, 17 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 19, 17 -- 12/07/2006 02:19:09 PM 19, 17 -- MIME-Version: 1.0 19, 17 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Post Doctoral Position in the NCSU Analytical Instrumentation Facility SIMS Laboratory
The Analytical Instrumentation Facility (AIF) of the NC State University College of Engineering has an opening for a postdoctoral research associate in its SIMS laboratory. The successful candidate will operate our magnetic sector IMS-6f magnetic sector and our PHI TRIFT I SIMS instruments. The successful candidate must be interested in learning and participating in the analysis of a wide variety of samples (semiconductors, metals, ceramics, polymers). Work in the laboratory will consist of a combination of SIMS analytical technique research, experimental design, instrument operation and data interpretation for university and industrial researchers. Training in SIMS and other analytical techniques and instrumentation (AFM, FIB, SEM, XPS, optical and stylus profilometry etc.) will be provided as needed for supporting SIMS research and analysis. For information on AIF, see our web site: http://www.ncsu.edu/aif/. A Ph.D in Analytical Chemistry, Applied Physics, Materials Science or a materials related discipline along with hands on experience with SIMS or other surface analysis or ion beam instrumentation is required. SIMS experience is highly desirable although training will be given to a suitable applicant. Experience in UHV equipment and/or electronics maintenance; operational and programming experience with Windows and Unix operating systems; and experience with analysis of semiconductor or other non biological materials are desirable.
Please apply on line at http://jobs.ncsu.edu ( please search position # 04-31-0601). Applicants will need to submit a cover letter, curriculum vitae and the names and addresses of three references. Review of applications will begin 1/08/2007, and this position will remain open until a suitable candidate is identified. NC State University is an OEO/AA employer. NC State welcomes all persons without regard to sexual orientation. ADA individuals desiring reasonable accommodations call 919-515-3148.
==============================Original Headers============================== 4, 19 -- From dale_batchelor-at-ncsu.edu Thu Dec 7 15:34:50 2006 4, 19 -- Received: from uni07mr.unity.ncsu.edu (uni07mr.unity.ncsu.edu [152.1.224.166]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB7LYoFf008807 4, 19 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 7 Dec 2006 15:34:50 -0600 4, 19 -- Received: from [152.14.52.68] (dbmacx.aif.ncsu.edu [152.14.52.68]) 4, 19 -- by uni07mr.unity.ncsu.edu (8.13.7/8.13.8/Nv5.2006.1109) with ESMTP id kB7LYmWI019058 4, 19 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 7 Dec 2006 16:34:49 -0500 (EST) 4, 19 -- Mime-Version: 1.0 (Apple Message framework v624) 4, 19 -- Content-Transfer-Encoding: 7bit 4, 19 -- Message-Id: {2d570ae9e86b73e2cd5e3cbe7707ae11-at-ncsu.edu} 4, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 19 -- To: Microscopy-at-msa.microscopy.com 4, 19 -- From: Dale Batchelor {dale_batchelor-at-ncsu.edu} 4, 19 -- Subject: [Microscopy] Post Doc Position 4, 19 -- Date: Thu, 7 Dec 2006 16:35:24 -0500 4, 19 -- X-Mailer: Apple Mail (2.624) 4, 19 -- X-PMX-Version: 5.2.1.279297, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2006.12.7.131933 4, 19 -- X-Spam-Status: No, Hits=7% 4, 19 -- X-Spam-Level: IIIIIII ==============================End of - Headers==============================
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Email: burrmich-at-msu.edu Name: Mike
Title-Subject: [Filtered] Sample prep for gels
Question: Can someone help me and suggest some good techniques for SEM/EDS analysis of gels. More specifically, i have a silicone based gel that i would like to run an EDS scan on without contaminating the chamber.
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Email: coridan-at-uiuc.edu Name: Robert Coridan
Organization: UIUC, IL, USA
Title-Subject: [Filtered] Vitrobot problems -- Base cracking?
Question: Hi---
We recently purchased an FEI Vitrobot and have had some success using it, but there are 2 problems that make working with it difficult.
1) The plungerod sticks: On ths model, there is a horizontal (left-to-right) adjustment to allow the operator to adjust the guidance of the plungerod. However, it seems that some front-and-back adjustments are required too. When we drop the plungerod, it is sometimes slowed (or stopped middrop) by the sliding washer because the rod is too far forward.
2) The foam base has cracked irreparably twice in the 2 months we have been using it. This is a newer design for the vitrobot base: the foam holds the LN2 and also insulates the brass ethane cup. We are using it according to the directions, but this foam is breaking more frequently than it should.
I have contacted FEI, but I also thought I'd check to see if any other Vitrobot users have experienced any of these problems.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both john.grace-at-abbott.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: john.grace-at-abbott.com Name: John Grace
Organization: Abbott Laboratories
Title-Subject: [Filtered] Frame Grabber boards for PCI Express Computers
Question: All,
I am replacing my aging G4 Image processing workstation. I have a Scion CG-7 framegrabber board, this will not work in the newer Mac workstations with PCI Express slots. Are there any framegrabbers that will work with Image j and a newer Mac available? Should I just get a PC with PCI slots?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mahonys-at-agr.gc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mahonys-at-agr.gc.ca Name: Shannon Mahony
Organization: Agriculture and Agri-Food Canada
Title-Subject: [Filtered] Imaging of structures in glycerin
Question: Hello all,
I have been attempting to take pictures (with the microscope) of insect genitalia in glycerin. I have seen excellent, sharp results before using this method. However, I am finding that even using a Z-stack and montaging the images with AutoMontage, I am not getting a clear, sharp result. I am confident that this is not due to vibration during the imaging. Does anyone else have any suggestions? Do I need to make specific adjustments because of the glycerin (i.e. aperture) or anything like that?
Mohammed's question ramifies much further than it seems at first sight. Most physics students tend to be dropped into the quantum quagmire without being shown how it relates to earlier stuff. I will give a brief history here:
In the classical world, Hero of Alexandria found that light, traveling from one point to another by a reflection from a plane mirror, always takes the shortest possible path.
What Hero did for reflection, Fermat did for refraction. He took Huygens’s view that light should travel more slowly in a denser medium, and reformulates the principle by postulating that the light travels in a path that takes the least time! In hindsight, if c is constant then Hero and Fermat are in complete agreement. Based on his reasoning, he is able to deduce both the law of reflection and Snell’s law (n1sinQ1 = n2sinQ2).
Unfortunately, this was not accepted. The continentals believed that light travelled faster in a denser medium. This is the same as Newton’s corpuscular theory of light, but that came later. Rather, it was accepting the philosophical conclusions of Descartes.
The PRINCIPLE OF LEAST ACTION. Maupertuis developed the principle in first in optics, to square the calculations of Fermat with the generally held belief that light travels faster in glass than air. He enunciated “In all changes that take place in the universe, the sum of products of the speed of each body and the distance it moves is the least possible.”
This worked, so he extended it to mechanics. [[Note for future reference that energy × time is the units of Planck’s constant.]] He didn’t give it a very solid math background, though, but what did for him was giving a theological rider “Action is minimized through the Wisdom of God”. This led Voltaire to satirize him, driving him out of Paris, and he died on the way to Berlin.
Euler picked up the principle and gave it sound mathematics, but being the generous man that he was, he ascribed the principle itself to Maupertuis. In 1755 the young Lagrange wrote to Euler with a much slicker (in the best sense) mathematical formulation, and Euler dropped his own formulation and took up Lagrange's. From this we get LAGRANGIAN mechanics.
In the early 1800's Thomas Young demonstrated the wave nature of light, and the corpuscular theory was swept under the carpet.
Around 1830 William Rowan Hamilton was developing a more advanced treatment of optics based on the wave formulation. He extended this to mechanics, and in 1834-5 he published two papers on which it is possible to base all of mechanics and most of classical physics. In 1857 the English mathematician Arthur Cayley gave this formulation the name HAMILTONIAN. It is the ancestor of the Hamiltonian in quantum mechanics.
Louis de Broglie is famous among physicists for giving us the first wave theory of the electron in the doctoral thesis in 1924. In his memoirs he states “ The theory of light was suffering from a strange illness, which took the form of an antagonistic dualism between the waves of Fresnel and Maxwell, one the one hand, and photons on the other”. … “Thus appeared the idea of extending to matter the corpuscles-waves duality which was essental to light”. ***** “The equation which expresses that the motion of an electron is quantised has … the following significance - that Maupertuis’ integral [sign]mvds taken along the closed trajectory is equal to an integral multiple of [Planck’s] constant h.”
----------------------------------- Robert H. Olley URL: http://www.rdg.ac.uk/~spsolley -----------------------------------
_________________________________________________________________ It's Hotmail's 10th Birthday! Come and play Pass the Parcel http://www.msnpasstheparcel.com
==============================Original Headers============================== 14, 21 -- From hinmeigeng-at-hotmail.com Fri Dec 8 09:18:54 2006 14, 21 -- Received: from bay0-omc2-s37.bay0.hotmail.com (bay0-omc2-s37.bay0.hotmail.com [65.54.246.173]) 14, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB8FIrsx028913 14, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 8 Dec 2006 09:18:53 -0600 14, 21 -- Received: from hotmail.com ([65.55.130.95]) by bay0-omc2-s37.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2668); 14, 21 -- Fri, 8 Dec 2006 07:18:53 -0800 14, 21 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 14, 21 -- Fri, 8 Dec 2006 07:18:52 -0800 14, 21 -- Message-ID: {BAY125-F15631D631515915344A32DCAD30-at-phx.gbl} 14, 21 -- Received: from 65.55.130.123 by by125fd.bay125.hotmail.msn.com with HTTP; 14, 21 -- Fri, 08 Dec 2006 15:18:46 GMT 14, 21 -- X-Originating-IP: [81.153.105.2] 14, 21 -- X-Originating-Email: [hinmeigeng-at-hotmail.com] 14, 21 -- X-Sender: hinmeigeng-at-hotmail.com 14, 21 -- From: "Robert H. Olley" {hinmeigeng-at-hotmail.com} 14, 21 -- To: Microscopy-at-MSA.Microscopy.Com 14, 21 -- Subject: Optical Physics 14, 21 -- Date: Fri, 08 Dec 2006 15:18:46 +0000 14, 21 -- Mime-Version: 1.0 14, 21 -- Content-Type: text/plain; format=flowed 14, 21 -- X-OriginalArrivalTime: 08 Dec 2006 15:18:52.0981 (UTC) FILETIME=[2B974650:01C71ADC] ==============================End of - Headers==============================
On Dec 7, 2006, at 3:59 PM, coridan-at-uiuc.edu wrote:
} 1) The plungerod sticks: On ths model, there is a horizontal } (left-to-right) adjustment to allow the operator to adjust the } guidance of the plungerod. However, it seems that some front-and-back } adjustments are required too. When we drop the plungerod, it is } sometimes slowed (or stopped middrop) by the sliding washer because } the rod is too far forward. } } 2) The foam base has cracked irreparably twice in the 2 months we have } been using it. This is a newer design for the vitrobot base: the foam } holds the LN2 and also insulates the brass ethane cup. We are using } it according to the directions, but this foam is breaking more } frequently than it should. } } I have contacted FEI, but I also thought I'd check to see if any other } Vitrobot users have experienced any of these problems. } Dear Rob, We have not had any problem with the plungerod on our Vitrobot, but we have had a problem with the cryogen cup occasionally moving forward even though the "semi-automated specimen transfer" box is not checked. This could cause the rod to become misaligned, but we have been lucky in that respect. I think our model does not have a plungerod adjustment that the user can operate. Several of our cryogen cups have cracked, so we always keep a spare. FEI is aware of the problem, and we are awaiting a solution. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Fri Dec 8 11:29:09 2006 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB8HT9rs021338 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 8 Dec 2006 11:29:09 -0600 4, 22 -- Received: from water-dog.caltech.edu (water-dog [192.168.1.26]) 4, 22 -- by earth-ox-postvirus (Postfix) with ESMTP id CCD5710A2A2 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 8 Dec 2006 09:29:08 -0800 (PST) 4, 22 -- Received: from [192.168.159.233] (jpix-01.caltech.edu [131.215.2.133]) 4, 22 -- by earth-ox.its.caltech.edu (Postfix) with ESMTP id E8BF510A2AE 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 8 Dec 2006 09:29:05 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v624) 4, 22 -- In-Reply-To: {200612072359.kB7Nx18t024356-at-ns.microscopy.com} 4, 22 -- References: {200612072359.kB7Nx18t024356-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {59df446f9d4362dde424aa7b07b4aac2-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] viaWWW: Vitrobot problems -- Base cracking? 4, 22 -- Date: Fri, 8 Dec 2006 09:35:13 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.624) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.3.3 ==============================End of - Headers==============================
Most "frost free" freezers have a timer that controls a heating element. The heating element is turned on briefly which warms the freezer enough to melt the frost which has formed. A tube carries any melt water to an evaporation pan found usually under the freezer. This means that the temperature inside the freezer cycles through warmer and colder phases. This temperature cycling is probably the cause of the loss of enzyme activity. Some influenza vaccine manufacturers provide special containers for vaccines where frost free freezers will be used. These containers stabilize the temperature inside and prolong the vaccine's life. A similar insulated box would likely prolong the enzyme activity.
Bob Carter 2000 Bayshore Road Lopez Island, WA 98261-8595
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Hi,
I have 2 "blöde" questions, not directly related with microscopy, but perhaps one of you can still answer them:
1) I stored LM cryo-sections (unfixed, 5 µm thick) at -28°C for approx. 5 months and now I test the activity of alkaline phosphatase (very positive at the moment of cutting) I see no signal. Control show very strong signal, so it does not come from the technique. I wonder how I lost the enzyme activity. I know for proteins it is better to store à -80°C but I wouldn't expect to lose all the activity! The freezer is a "frost-free" freezer. Does someone know how these freezers work? Could the mechanism of frost-free freezers be detrimental to the enzymatic activity?
2) For the cell line OE21 in the ECACC catalog, I see "Hazard: CX". No other information! I couldn't find this cell line in the ATCC catalog and I couldn't find an explanation on the internet. What does it mean, CX?
Stephane
==============================Original Headers============================== 12, 32 -- From bob-at-rockisland.com Fri Dec 8 12:26:41 2006 12, 32 -- Received: from mars.rockisland.com (dnscache2.rockisland.com [64.119.0.12]) 12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kB8IQeAA000754 12, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Dec 2006 12:26:40 -0600 12, 32 -- Received: from localhost (localhost [127.0.0.1]) 12, 32 -- by localhost.rockisland.com (Postfix) with ESMTP id 66DC85E49 12, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Dec 2006 10:26:40 -0800 (PST) 12, 32 -- (envelope-from bob-at-rockisland.com) 12, 32 -- X-Virus-Scanned: amavisd-new at rockisland.com 12, 32 -- Received: from mars.rockisland.com ([127.0.0.1]) 12, 32 -- by localhost (smtp.rockisland.com [127.0.0.1]) (amavisd-new, port 10024) 12, 32 -- with ESMTP id 4eiqsKXfgDiP for {Microscopy-at-microscopy.com} ; 12, 32 -- Fri, 8 Dec 2006 10:26:40 -0800 (PST) 12, 32 -- Received: from housecat (adsl-sj-15-153.rockisland.net [64.119.15.153]) 12, 32 -- by mars.rockisland.com (Postfix) with SMTP id 137035E47 12, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Dec 2006 10:26:40 -0800 (PST) 12, 32 -- (envelope-from bob-at-rockisland.com) 12, 32 -- Message-ID: {001901c71af6$6bcfb580$0302a8c0-at-housecat} 12, 32 -- From: "Bob Carter" {bob-at-rockisland.com} 12, 32 -- To: {Microscopy-at-microscopy.com} 12, 32 -- Subject: RE: 2 questions not directly relateed to microscopy 12, 32 -- Date: Fri, 8 Dec 2006 10:26:47 -0800 12, 32 -- MIME-Version: 1.0 12, 32 -- Content-Type: text/plain; 12, 32 -- format=flowed; 12, 32 -- charset="iso-8859-1"; 12, 32 -- reply-type=original 12, 32 -- Content-Transfer-Encoding: 8bit 12, 32 -- X-Priority: 3 12, 32 -- X-MSMail-Priority: Normal 12, 32 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2869 12, 32 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2962 ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (etienne.rivest-at-sympatico.ca) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, December 11, 2006 at 10:06:09 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both etienne.rivest-at-sympatico.ca as well as to the Microscopy Listserver ---------------------------------------------------------------------------
I am in the process of buying a microscope but, while browsing the web from ebay to majors manufacturers, I noted the wide variety of available items, specifications and price range. This poses some questions and I was glad to find your site that is the first ressource on my way that offers to give answers.
The first one is: what scope should I buy? Well, my goals in obtaining a microscope are primarely to observe potential bacterial activiy in home processed food preserves to establish preserving protocols (pressure canner sterelization, etc). The second is to observe beer yeasts in culture to determine its vitality (and purity of strain if possible). Lastly, to observe anything in the micro world for the only and pure fun of discovering hidden aspects of life and nature.
To clarify a bit, I would like to add that the more I read about microscopy (power range, types of eyepieces, objectives and condensers, numerical apperture, etc) the more I get confused. So, to be able to achieve my main expectations, what characteistics should I consider essential, considering that the microscope must also be financially affordable?
Thes last part of the preceding question leads to this one: beside the major makers who manufacture superb microscopes that I can in no way offer to myself, I saw lots of China (I guess) made kind of copies priced for less then half (when not the third) the price of what the majors ask. That's the case on ebay and a couple of sites I visited. Are those scopes adequate for private amateur home use? Would you consider it better not to have any microscope at all for a some years instead of having one of those in a few months or weeks?
Finally, what is the best way to find the microscope I need: new or used? popular sellers (like ebay)? Can you recommend a brand (or brands), a web site? I live in the country, relatively far from major cities, which is why I'm shoping through the web.
I thank you very much to offer the opportunity to ask you my questions. Thank you in advance for your help.
I am trying to help a colleague with autoradiography on a cancer project. I was intending to cut t 0.5 - 1.0 um cross sections of the tumors after embedding them in BMMA (butylmethylmethacrylate) which is a lot like JB-4 but easily extractable. I just learned that the tumors are bigger than I expected - between 1 and 2 cm. I need to cut the full width cross section since we are looking for distribution of a drug. I have always cut methacrylate resins on a standard ultramicrotome. I have heard but not seen a so-called JB-4 microtome. Is this a standard microtome with a metal blade or is it different? The lab I will be working in can get a Reichert Supercut 2050 microtome to use on this project. I see literature references where this microtome was used to cut JB-4. Is anyone familiar with this model? Is it a standard microtome with either disposable razor blade or paraffin microtome blade? Does anyone know how big (wide) a block you can cut on a standard microtome? Thanks, Tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
Has anyone cleaned the oil out of an oil mist filter from a rotary pump from an Hitachi TEM? Or any similar filter? The pump uses mineral oil. The instructions say to use Perkleen or similar defatting agent; don't use solvents. However I've never heard of Perkleen and have no idea what might be in it. Any suggestions?
Diana
==============================Original Headers============================== 3, 18 -- From dianavd-at-eye.usyd.edu.au Mon Dec 11 16:09:41 2006 3, 18 -- Received: from galen.med.usyd.edu.au (nugalen.med.usyd.edu.au [129.78.36.39]) 3, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBBM9fo9014444 3, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Dec 2006 16:09:41 -0600 3, 18 -- Received: from [129.78.203.144] (helo=[129.78.203.144]) 3, 18 -- by galen.med.usyd.edu.au with esmtp (Exim 4.43) 3, 18 -- id 1GttL9-0001tc-Ts 3, 18 -- for Microscopy-at-microscopy.com; Tue, 12 Dec 2006 09:09:40 +1100 3, 18 -- Mime-Version: 1.0 (Apple Message framework v752.2) 3, 18 -- Content-Transfer-Encoding: 7bit 3, 18 -- Message-Id: {15DFD0A3-0912-454D-A749-E69250CC17C1-at-eye.usyd.edu.au} 3, 18 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 3, 18 -- To: Microscopy {Microscopy-at-microscopy.com} 3, 18 -- From: Diana van Driel {dianavd-at-eye.usyd.edu.au} 3, 18 -- Subject: cleaning oil mist filter 3, 18 -- Date: Tue, 12 Dec 2006 09:09:34 +1100 3, 18 -- X-Mailer: Apple Mail (2.752.2) 3, 18 -- X-Spam-Score: -3.3 (---) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both qfxingtem-at-hotmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] High Tension Stability of Tecnai-F20ST
Question: Dear Listers:
Does anyone know the high-tension stability (change of the accelerating voltage versus time) of Tecnai-F20ST after setting to the voltage for 3 hours? I used 120kV. The nominal value at 200kV is aslo okay.
O} } Hi all, } } Has anyone cleaned the oil out of an oil mist filter from a rotary } pump from an Hitachi TEM? Or any similar filter? The pump uses } mineral oil. The instructions say to use Perkleen or similar } defatting agent; don't use solvents. However I've never heard of } Perkleen and have no idea what might be in it. Any suggestions? } } Diana }
I would guess that it's a trade name for perchloroethylene, which has been and still may be being used as a drycleaning solvent. It is, of course, a solvent, heaven only knows what they mean by 'don't use a solvent'. Like all halogenated solvents, it isn't a nice thing to absorb through the skin of your hands or your lungs.
cheers
rtch
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 6, 27 -- From r.sims-at-auckland.ac.nz Mon Dec 11 19:24:38 2006 6, 27 -- Received: from chico.itss.auckland.ac.nz (chico.itss.auckland.ac.nz [130.216.190.12]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBC1OaQa015014 6, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Dec 2006 19:24:37 -0600 6, 27 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 6, 27 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id 0D70C345B6; 6, 27 -- Tue, 12 Dec 2006 14:24:33 +1300 (NZDT) 6, 27 -- Received: from chico.itss.auckland.ac.nz ([127.0.0.1]) 6, 27 -- by localhost (smtpb.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 6, 27 -- with ESMTP id 10372-02; Tue, 12 Dec 2006 14:24:32 +1300 (NZDT) 6, 27 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) 6, 27 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id D96C2343F3; 6, 27 -- Tue, 12 Dec 2006 14:24:31 +1300 (NZDT) 6, 27 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 6, 27 -- To: dianavd-at-eye.usyd.edu.au 6, 27 -- Date: Tue, 12 Dec 2006 14:29:37 +1300 6, 27 -- MIME-Version: 1.0 6, 27 -- Subject: Re: [Microscopy] cleaning oil mist filter 6, 27 -- Cc: Microscopy-at-microscopy.com 6, 27 -- Message-ID: {457EBCD1.25576.174E2CD-at-localhost} 6, 27 -- Priority: normal 6, 27 -- In-reply-to: {200612112210.kBBMAbnJ016111-at-ns.microscopy.com} 6, 27 -- X-mailer: Pegasus Mail for Windows (4.21c) 6, 27 -- Content-type: text/plain; charset=US-ASCII 6, 27 -- Content-transfer-encoding: 7BIT 6, 27 -- Content-description: Mail message body 6, 27 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
2050s are heavy duty motorised microtomes designed to cope with the high cutting forces associated with resin sectioning so should suit your purpose well, assuming you can accommodate a glass, diamond or a tungsten carbide blade. They can also accomodate standard wax sectioning steel and disposable blades. All of these options depend on the type of knife stage your 2050 is fitted with.
I don't think that standard blades would stand up to sectioning large MMA blocks, but listers may know otherwise.
I regularly use a 2050 with glass triangular knives with a cutting edge the thickness of the glass (1cm) to cut GMA and MMA blocks 1-2 cm long - positioning the block with the narrow edge meeting the knife and with its leading edge trimmed to a point. This allows a gradual introduction of the cutting force, and gives you something to pick up with fine forceps without damaging the tissue if cutting the sections dry.
Tungsten carbide blades also work well for larger blocks. I have found that MMA sections best if you wet the block and blade with 70% EtOH and smooth the section flat with a fine artist brush before transferring to a water bath for collecting onto slides.
As MMA sections can be floated direct onto water, we use a histodiamond to provide best quality sections having first trimmed in with a glass or TC blade.
Get in touch if you need further info.
Good luck,
Alastair
At 15:23 11/12/2006 -0600, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Alastair McKinnon Histology & EM Facility Manager University of Aberdeen, Institute of Medical Science Foresterhill, Aberdeen, AB25 2ZD tel: +44(0) 1224 552923 (office); +44(0) 1224 555911 (lab) fax: +44(0) 1224 559533; email: a.d.mckinnon-at-abdn.ac.uk www.abdn.ac.uk/ims/h-em
==============================Original Headers============================== 16, 18 -- From a.d.mckinnon-at-abdn.ac.uk Tue Dec 12 03:19:20 2006 16, 18 -- Received: from mailhub2.abdn.ac.uk (mailhub2.abdn.ac.uk [139.133.7.24]) 16, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBC9JJGd004211 16, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Dec 2006 03:19:20 -0600 16, 18 -- Received: from clsm-002230.ims.abdn.ac.uk ([139.133.169.205] helo=CLSM-002230.abdn.ac.uk) 16, 18 -- by mailhub2.abdn.ac.uk with esmtp (Exim 4.52) 16, 18 -- id 1Gu3nC-0007hi-Fv; Tue, 12 Dec 2006 09:19:19 +0000 16, 18 -- Message-Id: {5.2.1.1.0.20061212084505.011d2040-at-mailms.abdn.ac.uk} 16, 18 -- X-Sender: pat081-at-mailms.abdn.ac.uk 16, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 16, 18 -- Date: Tue, 12 Dec 2006 09:19:15 +0000 16, 18 -- To: phillipst-at-missouri.edu 16, 18 -- From: Alastair McKinnon {a.d.mckinnon-at-abdn.ac.uk} 16, 18 -- Subject: Re: [Microscopy] JB-4, BMMA, Supercut 2050 microtome questions 16, 18 -- Cc: Microscopy-at-microscopy.com 16, 18 -- In-Reply-To: {200612112123.kBBLNsbs010977-at-ns.microscopy.com} 16, 18 -- Mime-Version: 1.0 16, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Have your voice heard among your peers and experts in nanotechnology!
Seeing at the Nanoscale, the fifth annual scientific conference focusing on nanostructural imaging, characterization, and modification using scanning probe microscopy (SPM) and related techniques, is now accepting papers for presentation consideration.
The conference location is Santa Barbara, California, June 24-27, 2007. Sponsored by Veeco Instruments and the California NanoSystems Institute (CNSI) at the University of California, Santa Barbara (UCSB), this two-and-one-half day event includes technical presentations, a nanotechnology poster contest, and a beach barbecue—Santa Barbara style.
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==============================Original Headers============================== 14, 20 -- From MCarlyle-at-veeco.com Tue Dec 12 09:27:04 2006 14, 20 -- Received: from sboexch2.int.veeco.com (SBOEXCH2.VEECO.com [68.111.35.167]) 14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBCFR3FW031607 14, 20 -- for {Microscopy-at-Microscopy.Com} ; Tue, 12 Dec 2006 09:27:03 -0600 14, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 14, 20 -- content-class: urn:content-classes:message 14, 20 -- MIME-Version: 1.0 14, 20 -- Content-Type: text/plain; 14, 20 -- charset="utf-8" 14, 20 -- Subject: AFM -- Seeing at the Nanoscale V Conference Call for Papers 14, 20 -- Date: Tue, 12 Dec 2006 07:27:02 -0800 14, 20 -- Message-ID: {625BD6DD96DE554DBB0558FFE70E0F420120016E-at-sboexch2.int.veeco.com} 14, 20 -- X-MS-Has-Attach: 14, 20 -- X-MS-TNEF-Correlator: 14, 20 -- Thread-Topic: AFM -- Seeing at the Nanoscale V Conference Call for Papers 14, 20 -- Thread-Index: AcceAfjYH90V77/ITkSawDkMxJD/Xg== 14, 20 -- From: "Marlene Carlyle" {MCarlyle-at-veeco.com} 14, 20 -- To: "Microscopy-at-Microscopy.Com" {'Microscopy-at-Microscopy.Com'} 14, 20 -- Content-Transfer-Encoding: 8bit 14, 20 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id kBCFR3FW031607 ==============================End of - Headers==============================
---------------------------------------------------------------------- FOCUS ON MICROSCOPY 2007 -- Valencia, Spain -- April 10-13, 2007 ----------------------------------------------------------------------
20th International Conference on 3D Image Processing in Microscopy 19th International Conference on Confocal Microscopy
Dear Colleagues,
January 15, 2007, the deadline for abstract submission for the Valencia FOM2007 conference is nearing. Please submit your abstract by that date.
The program for the conference will be finalized and available on our website on Feb. 6, 2007. Authors will be informed individually by E-mail on the placement of their contribution in the program. Abstracts for oral and poster presentations are sollicited. Submission preferably through the conference website: http://focusonmicroscopy.org/ where also the conference registration is open and hotel booking information is available.
After the successful FOM2006 conference held in Perth in April this year, the next conference: Focus on Microscopy 2007 will take place in Valencia, Spain from Tuesday April 10 to Friday April 13th, 2007. Please note that this is in the week after Easter 2007. As the next in a series of unique interdisciplinary meetings on advanced multidimensional light microscopy and image processing, the conference will be hosted by the University of Valencia.
Focus on Microscopy 2007 is the continuation of a conference series, started in 1988, presenting the latest innovations in optical microscopy and their application in biology, medicine and the material sciences. Key subjects for the conference are the theory and practice of 3D optical imaging, related 3D image processing, together with reporting on the ever increasing spatial resolutions and sophisticated imaging modes coming available in sectioning microscopy.
The conference series is in addition known for covering the rapid development of advanced fluorescence labeling techniques for confocal and multi-photon 3D imaging of -live- biological specimens. Laser light in combination with 3D microscopy is starting to play an increasingly important role as a tool at the sub micrometer scale in cell biology for dissection and isolation of structures of interest for subsequent analysis. Specific sessions on this subject and advances in endoscopic and non-invasive imaging are planned for FOM2007.
Important dates: Deadline for the submission of abstracts: January 15, 2007 Acceptance of contributions, draft program: February 6, 2007 Deadline for early registration: February 26, 2007
To stay informed about the conference please leave your name and email at http://www.FocusOnMicroscopy.org/stayinformed
Welcoming you to Valencia for the FOM2007 conference and exhibition, on behalf of the organising committee,
- Manuel Martinez-Corral, University of Valencia, Spain - Fred Brakenhoff, University of Amsterdam, The Netherlands -- E-mail: info2007-at-FocusOnMicroscopy.org Web: www.FocusOnMicroscopy.org
==============================Original Headers============================== 16, 27 -- From brakenho-at-science.uva.nl Tue Dec 12 10:30:07 2006 16, 27 -- Received: from imap.science.uva.nl (imap.science.uva.nl [146.50.4.51]) 16, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBCGU61b011653 16, 27 -- for {microscopy-at-msa.microscopy.com} ; Tue, 12 Dec 2006 10:30:07 -0600 16, 27 -- Received: from webmail.science.uva.nl [146.50.4.91] 16, 27 -- by imap.science.uva.nl with ESMTP (sendmail 8.11.6p2/config 11.38). 16, 27 -- id kBCGU5M11482; Tue, 12 Dec 2006 17:30:05 +0100 16, 27 -- X-Organisation: Faculty of Science, University of Amsterdam, The Netherlands 16, 27 -- X-URL: http://www.science.uva.nl/ 16, 27 -- Received: from localhost ([127.0.0.1]) 16, 27 -- (SquirrelMail authenticated user brakenho) 16, 27 -- by webmail.science.uva.nl with HTTP; 16, 27 -- Tue, 12 Dec 2006 17:30:05 +0100 (CET) 16, 27 -- Message-ID: {50121.145.18.160.47.1165941005.squirrel-at-webmail.science.uva.nl} 16, 27 -- Date: Tue, 12 Dec 2006 17:30:05 +0100 (CET) 16, 27 -- Subject: FOM2007 Abstract deadline 15 Jan. Valencia, Spain, April 10-13, 16, 27 -- Focus on Microscopy, Registration 16, 27 -- From: "Fred Brakenhoff" {brakenho-at-science.uva.nl} 16, 27 -- To: microscopy-at-msa.microscopy.com 16, 27 -- Cc: brakenhoff-at-science.uva.nl 16, 27 -- User-Agent: SquirrelMail/1.4.6-rc1 16, 27 -- MIME-Version: 1.0 16, 27 -- Content-Type: text/plain;charset=iso-8859-1 16, 27 -- Content-Transfer-Encoding: 8bit 16, 27 -- X-Priority: 3 (Normal) 16, 27 -- Importance: Normal 16, 27 -- X-Virus-Scanned: by amavisd-new ==============================End of - Headers==============================
The JB-4 is a retracting motorized microtome designed for semi-thin sections. Depending upon the available knife holders and your sample, it will use disposable steel, triangular glass, Ralph knives, tungsten carbide or diamond knives. We've used glass knives and Histodiamonds with ours to cut epoxy and methacrylate sections, 0.5 to 5 µm thick. As, Alastair says, a razor blade or a standard steel blade won't survive what you want to cut. That plastic will curl the edge or even pull out the metal.
Regards, Glen } } } --------------------------------------------------------------------- } } ------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------- } } ------- } } } } I am trying to help a colleague with autoradiography on a cancer } } project. I } } was intending to cut t 0.5 - 1.0 um cross sections of the tumors } } after } } embedding them in BMMA (butylmethylmethacrylate) which is a lot } } like JB-4 } } but easily extractable. I just learned that the tumors are bigger } } than I } } expected - between 1 and 2 cm. I need to cut the full width cross } } section } } since we are looking for distribution of a drug. I have always cut } } methacrylate resins on a standard ultramicrotome. I have heard } } but not } } seen a so-called JB-4 microtome. Is this a standard microtome } } with a metal } } blade or is it different? The lab I will be working in can get a } } Reichert } } Supercut 2050 microtome to use on this project. I see literature } } references } } where this microtome was used to cut JB-4. Is anyone familiar } } with this } } model? Is it a standard microtome with either disposable razor } } blade or } } paraffin microtome blade? Does anyone know how big (wide) a block } } you can } } cut on a standard microtome? Thanks, Tom } } } } Thomas E. Phillips, PhD } } Professor of Biological Sciences } } Director, Molecular Cytology Core } } 2 Tucker Hall } } University of Missouri } } Columbia, MO 65211-7400 } } } } 573-882-4712 (office) } } 573-882-0123 (fax) } } PhillipsT-at-missouri.edu } }
==============================Original Headers============================== 4, 27 -- From glenmac-at-u.washington.edu Tue Dec 12 12:42:42 2006 4, 27 -- Received: from mxout3.cac.washington.edu (mxout3.cac.washington.edu [140.142.32.166]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBCIgg5j025805 4, 27 -- for {microscopy-at-microscopy.com} ; Tue, 12 Dec 2006 12:42:42 -0600 4, 27 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.139]) 4, 27 -- by mxout3.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW06.09) with ESMTP id kBCIgf7j004248 4, 27 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 4, 27 -- for {microscopy-at-microscopy.com} ; Tue, 12 Dec 2006 10:42:41 -0800 4, 27 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) 4, 27 -- (authenticated authid=glenmac) 4, 27 -- by smtp.washington.edu (8.13.7+UW06.06/8.13.7+UW06.09) with ESMTP id kBCIgfPG001114 4, 27 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 4, 27 -- for {microscopy-at-microscopy.com} ; Tue, 12 Dec 2006 10:42:41 -0800 4, 27 -- Mime-Version: 1.0 (Apple Message framework v752.2) 4, 27 -- In-Reply-To: {200612120921.kBC9LRph006717-at-ns.microscopy.com} 4, 27 -- References: {200612120921.kBC9LRph006717-at-ns.microscopy.com} 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 4, 27 -- Message-Id: {749F3D15-7E41-447D-AB21-DFFECCAE58B7-at-u.washington.edu} 4, 27 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 4, 27 -- Subject: Re: [Microscopy] Re: JB-4, BMMA, Supercut 2050 microtome questions 4, 27 -- Date: Tue, 12 Dec 2006 10:42:35 -0800 4, 27 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} 4, 27 -- X-Mailer: Apple Mail (2.752.2) 4, 27 -- X-PMX-Version: 5.2.2.285561, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2006.12.12.102932 4, 27 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P1_5 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BADTHINGS 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' 4, 27 -- Content-Transfer-Encoding: 8bit 4, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kBCIgg5j025805 ==============================End of - Headers==============================
Thanks for all the recent advice on my question on the 2050 microtome and cutting JB-4 and BMMA. Today's question concerns squirt bottles for delivering ethanol (50, 70, 95 and 100%) to vials during the dehydration step prior to infiltration in resin. I have a set of squirt bottles with locking seals that are on their last legs and need to break down and get a net set. Does anyone know of an especially good type of squirt bottle that keeps the ethanol series from taking in water from the atmosphere? And yes, I know all about using dry ethanol and sieves, etc and that isn't part of this question! Thanks in advance, Tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
Hello, We are still using Vestopal W for embedding of our samples. During the years well polymerized blocks were transparent pink (aprox. color: #ffc0cb in hexadecimal) but now the polymerized block are transparent khaki to green. We have bought new tertiary butyl perbenzoate and cobalt naphthenate but the new mixture of Vestopal again polymerized in transparent khaki to green color. We have been using standard protocol temperature curring (60 oC) for years and we have never before observed such change in color of polymerized blocks. Please, could anybody explain it?
Thanking you in advance Oldrich
------------------------------------------ Oldrich Benada Institute of Microbiology, Acad. Sci. CR Videnska 1083 142 20 Praha 4 - Krc Czech Republic
==============================Original Headers============================== 4, 20 -- From benada-at-biomed.cas.cz Wed Dec 13 03:00:21 2006 4, 20 -- Received: from biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBD90KX1000564 4, 20 -- for {microscopy-at-microscopy.com} ; Wed, 13 Dec 2006 03:00:21 -0600 4, 20 -- Received: from [147.231.44.101] (u117ob.mbu.cas.cz [147.231.44.101]) 4, 20 -- by biomed.cas.cz (8.13.3+Sun/8.13.3) with ESMTP id kBD8xGsO004489 4, 20 -- for {microscopy-at-microscopy.com} ; Wed, 13 Dec 2006 09:59:16 +0100 (CET) 4, 20 -- From: benada-at-biomed.cas.cz 4, 20 -- To: microscopy-at-microscopy.com 4, 20 -- Date: Wed, 13 Dec 2006 10:00:16 +0100 4, 20 -- MIME-Version: 1.0 4, 20 -- Subject: Vestopal W question 4, 20 -- Message-ID: {457FCF30.29743.49651F-at-benada.biomed.cas.cz} 4, 20 -- Priority: normal 4, 20 -- X-mailer: Pegasus Mail for Windows (4.41) 4, 20 -- Content-type: text/plain; charset=US-ASCII 4, 20 -- Content-transfer-encoding: 7BIT 4, 20 -- Content-description: Mail message body 4, 20 -- X-Antivirus: avast! (VPS 0657-2, 12.12.2006), Outbound message 4, 20 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
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I've recently come into possesion of an old EM201. I was wondering if anyone out there has a PDF of the Installations manual (I have the other three, including Preinstallation). Also, does anyone know if the chilled water line can be filled with anything but water? I was wondering about an Ethanol solution or glycol (something with a depressed freezing point). It will be in a room that might get down to freezing temperatures if the electricity happens to fail. Will the seals stand up to chemicals other than water? Are there any other considerations for the cooling loop? Any help would be greatly appreciated. Thanks.
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Email: ht-at-nhm.ac.uk Name: Harry Taylor
Organization: NHM
Title-Subject: [Filtered] Wild M410 macroscope
Question: Hi there, Just wondering how easy it is to convert a Wild M410 macroscope body into the M420? I believe I just need to add the internal photo prism (my 410 already has the photo tube fitted) Does anybody know the part number of this item?......thanks in advance!
We get asked this fairly regularly by schools and parents (so we have stock replies). Often professional microscopists aren't the best source of information for cheap microscope's as all our optical systems are always over £10,000, and most lab mainstays like our three confocal microscopes come in at nearer £200,000 each (and users still complain about image quality). I assume you are asking about home use.
Cheap microscopes under £100 are always a disappointment and toy (often called student) microscopes can be very poor. You can easily buy second-hand via ebay, but again there are risks that the optics will be damaged or simply very dirty and difficult to clean and you may make an expensive mistake. Look for old branded 'laboratory' microscopes e.g. Bausch & Lomb, Prior, Leitz, Reichert, Baker, but not Tasco toys. Famous existing brands like Zeiss and Olympus attract a high premium. The sellers are often not microscopists though, and many are sold as collector's items and not intended for 'scientific' use. However many home users often get bored with new microscopes after a while, so there is a second hand market for cheaper school/college lab grade models. Also I would definitely try any local microscope enthusiast clubs - they aren't as common as the many excellent astronomy [telescope] clubs but they are about and have knowledgeable enthusiasts with an eye for low cost quality systems.
There are suppliers geared up to providing cheaper microscopes for schools & colleges, so you can ask around at school/college's science departments, but expect to pay nearer £500 each for a minimum quality setup (although with those like bottom end Meade [www.meade.com] at around £100 you can see something at low mag (~20x objective i.e. around 180x mag) with a quality stained section.
For pond life etc. a stereoscopic 'dissecting' microscope (40x to 120x mag) is ideal for home use. Also remember of course that you can get a really long way with a good large magnifying glass (not the really small hi-mag cheap folding lens ones, try before you buy) - I have a few excellent ones at home for £1 and a good low mag Osram one that includes an illuminating halogen bulb at £8. In general I would say a well made stereo dissecting microscope is a good choice (if not the best) for kids as it's great for viewing living things and enlarges what you can see already - look for 40x rather than 4x though. They are a bit expensive (£500+) though so you would probably have to buy secondhand (look out for branded ones like Bausch & Lomb, and Prior). These are ideal for colonies but unsuitable for viewing single cells where something like 400x to 600x is preferred (expensive 40 to 63x objectives), and requires a standard compound microscope.
Generally prepared slides can rapidly get very boring for all ages, but living or unusual things (even hamster fur) always attract an audience. Also try your flatbed film scanner, (not the LiDe types that have a very limited depth of focus, and any from around £60 upwards) that will be good for looking at soft static things: leaves, fruit, nuts, household objects (scan at max resolution and try reflected and 'film' mode). In the UK there are sites like http://www.wedgwood-group.com/microscopes_digital.htm that cater for schools and colleges, providing standard compound and stereo microscopes as well as cheap PC video based microscope solutions where the whole class of children can look at a computer screen with some pushing and shoving. Best to try them on 'approval' as many cheap microscopes can be very disappointing if you expect too much. For your use (living cells) you will probably want some form on contrast enhancement like Phase Contrast optics that adds to the complexity and expense (and starts to put the microscope in the £1,000+ category). Fixing & staining cells obviously reduces the need for any optical contrast enhancement.
Excellent pre-prepared stained slides of simple organisms, plant stems and leaves or bits of rats, insects etc.. can be bought via ebay, but they tend to be expensive and are easily broken by any age-group. Mounted slides keep well though, so 'vintage' ones even from 50 years ago can still look OK. Again school suppliers are geared up to provide for this sort of thing cheaply.
At home and our Primary school (under 11) we use the Digital Blue QX-5 (£70) - it's fun but pretty useless for serious microscope work as it's so low res (but at 640 x 480 better than the old Intel QX-3 it replaced). It puts the image on a PC screen. I have one at home for my kids (boy 10 and girl 12) but it only gets occasional use now the novelty has worn off. See http://micro.magnet.fsu.edu/optics/intelplay/index.html (not updated for the QX-5 but all applies - the site even discusses ways of contrast enhancement etc..). Once on the PC the 640x480 images can be manipulated and pasted etc, and the QX-5 does time-lapse for things like crystal growth. Living plants growing and small animals. This toy has nowhere near the resolution you require though. The similar but far better built Olympus MIC-D was great but being over £500 it was just too expensive for most schools and is now discontinued - there are other similar budget video microscope systems about though.
The macro on a good digital camera (like the image stabilised 5MP Canon S2IS - http://www.dpreview.com/reviews/canons2is)is also worth a try, particularly with a small tripod and halogen bendy desk lamp, if very close-up. I have an E500 digital SLR system with enlargement rings but they are more difficult to use. You can get quite reasonable pictures by resting a small compact digital camera lens against the eyepiece of a microscope. Plus you can use the camera for normal photography when you get bored with microscopy.
For details on your interest try books like the Bioaerosols Handbook by C.S. Cox. Granted this is for sampling bioaerosols (bacteria etc.. floating in air) not food as in your interest, but their must be similar books about for that. I mention the handbook as I wrote the chapter on microscopy and image analysis so I am very familiar with the book.
By the way, if you get a microscope, do try growing crystals on a slide, a few drops of a saturated solution of salt (NaCl) or copper sulphate will grow superb crystals on the surface of a slide when viewed under a microscope (but it takes a few hours for the crystals to form and they often look best before the liquids all gone). Just make sure you don't drive the objective tips into the solution. It's not biology but its fun (see http://micro.magnet.fsu.edu/.
Sorry I can't offer more specific help as our microscopes are rather more expensive than the one you wish to track down.
Regards
Keith
Try looking in Amazon.com for decent microscope books with lots of pictures - and they have a good customer review system for books and even microscopes (often viewing micrographs in books is better than trying to see it yourself down a microscope). Plus try web searches for general sites like these (and for more specific topics) :
http://www.101science.com/Microscope.htm http://micro.magnet.fsu.edu/ http://www.microscopy-uk.org.uk/index.html http://www.btinternet.com/~stephen.durr/ http://www.mccroneatlas.com http://www.diatoms.co.uk [for fun images made of diatoms]
Examples of sources for cheap school grade microscopes in the UK are:
Have an internet search for school/lab suppliers in Canada.
-----Original Message----- X-from: etienne.rivest-at-sympatico.ca [mailto:etienne.rivest-at-sympatico.ca] Sent: 11 December 2006 17:19 --------------------------------------------------------------------------- Please reply to both etienne.rivest-at-sympatico.ca as well as to the Microscopy Listserver ---------------------------------------------------------------------------
I am in the process of buying a microscope but, while browsing the web from ebay to majors manufacturers, I noted the wide variety of available items, specifications and price range. This poses some questions and I was glad to find your site that is the first ressource on my way that offers to give answers.
The first one is: what scope should I buy? Well, my goals in obtaining a microscope are primarely to observe potential bacterial activiy in home processed food preserves to establish preserving protocols (pressure canner sterelization, etc). The second is to observe beer yeasts in culture to determine its vitality (and purity of strain if possible). Lastly, to observe anything in the micro world for the only and pure fun of discovering hidden aspects of life and nature.
To clarify a bit, I would like to add that the more I read about microscopy (power range, types of eyepieces, objectives and condensers, numerical apperture, etc) the more I get confused. So, to be able to achieve my main expectations, what characteistics should I consider essential, considering that the microscope must also be financially affordable?
Thes last part of the preceding question leads to this one: beside the major makers who manufacture superb microscopes that I can in no way offer to myself, I saw lots of China (I guess) made kind of copies priced for less then half (when not the third) the price of what the majors ask. That's the case on ebay and a couple of sites I visited. Are those scopes adequate for private amateur home use? Would you consider it better not to have any microscope at all for a some years instead of having one of those in a few months or weeks?
Finally, what is the best way to find the microscope I need: new or used? popular sellers (like ebay)? Can you recommend a brand (or brands), a web site? I live in the country, relatively far from major cities, which is why I'm shoping through the web.
I thank you very much to offer the opportunity to ask you my questions. Thank you in advance for your help.
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Email: twigg-at-estd.nrl.navy.mil Name: Mark Twigg
Organization: Naval Research Laboratory
Title-Subject: [Filtered] Epoxy
Question: Generally my favorite epoxy for gluing down TEM specimens is Epotek H20E Silver Epoxy. This epoxy requires curing at about 120C, which is fine for most of my specimens. What I especially like about this epoxy is that it has a slightly pasty consistency, so that it does not suck the specimen in using capillary action. What I want to know is if there is another slightly pasty epoxy or glue out there which cures at room temperature. Some samples cannot endure curing at 120C, so it would be good have know of an alternative to Epotek H20E.
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Email: vchristie-at-altransolutions.com Name: Van Christie
Organization: Altran Solutions
Title-Subject: [Filtered] Zeiss vs JEOL
Question: We are ready to pull the trigger on a new VP SEM with an EDS system. I have decided on a Thermo EDS as I've had good experience with them. But the EM is a mystery. Both have great capabilities and similar features. The Zeiss is my first choice as in the VP mode, they have a vp secondary detector that will work with the BS detector. Four main boards control the whole instrument. One simple roughing pump, only a couple of valves and a turbo pump for HV.
So does anyone have experience with either of these newer machines? Any help is appreciated.
I am running a Zeiss EVO 40 with VPSE detector and am VERY happy with it (no analytical set-up). Our application is biological samples in biodiversity research (shells, tissues, resin casts, insects). When we wrote the NSF-MRI grant, I looked also at JEOL. JEOL came in second after the other common vendors for the following reasons. I selected the Zeiss for some of the reasons outlined below.
I do like the flexibility and ease of switching between modes, and that all variables can be continuously adjusted (15.00 kV or 15.01 kV is your choice, it is not 15 or 16 kV, and that for every parameter). Signal mixing (also continuous) is a great asset. I often do a 70-80% SE with 20-30% of 2 quadrants of BSE for "counter illumination".
No cooler required is another plus, just one less piece of equimpment to take care of. You also plug the instrument simply into a 110 outlet, no need getting 220/240V lines installed. So apart of the 4 boards, yet another way how the Zeiss is simple and robust.
Filament life is very good. If you check saturation about once a week, you can easily get 300-400 h out of a filament.
Macroprogramming is feasible, but not terribly intuive, but it can be done. I have some custom one-click-buttons for image taking (line integration, set of frame integration parameter pairs) with subsequent saving. Also did for one user a pull down-menue with standard magnifications.
Do get the keyboard with the adjustment buttons on it. You can then focus and magnify etc. at the same time. We do a bunch of student training and have visiting scientists, and the button-key-board makes it easier to get into the instrument.
Problems? We've had it running for 2 years with no significant down-time.
If you have any question please feel free to check back.
best wishes
Daniel
************************************************* Daniel L. Geiger, Ph.D. Research Curator of Electron Microscopy Santa Barbara Museum of Natural History - Invertebrate Zoology 2559 Puesta del Sol Road Santa Barbara, CA 93105 USA
On Thu Dec 14 5:47 , vchristie-at-altransolutions.com sent:
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==============================Original Headers============================== 2, 27 -- From geiger-at-vetigastropoda.com Thu Dec 14 10:00:03 2006 2, 27 -- Received: from st77.startlogic.com (st77.startlogic.com [72.22.71.31]) 2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kBEG02sD014797 2, 27 -- for {microscopy-at-microscopy.com} ; Thu, 14 Dec 2006 10:00:03 -0600 2, 27 -- Message-Id: {200612141600.kBEG02sD014797-at-ns.microscopy.com} 2, 27 -- Received: (qmail 78461 invoked by uid 3128); 14 Dec 2006 15:55:56 -0000 2, 27 -- Received: from 127.0.0.1 by st77.startlogic.com (envelope-from {geiger-at-vetigastropoda.com} , uid 1002) with qmail-scanner-1.25st 2, 27 -- (clamdscan: 0.88/1245. spamassassin: 3.1.0. perlscan: 1.25st. 2, 27 -- Clear:RC:1(127.0.0.1):SA:0(-0.4/5.0):. 2, 27 -- Processed in 2.156397 secs); 14 Dec 2006 15:55:56 -0000 2, 27 -- X-Spam-Status: No, hits=-0.4 required=5.0 2, 27 -- Received: from unknown (HELO st77.startlogic.com.com) (127.0.0.1) 2, 27 -- by st77.startlogic.com with SMTP; 14 Dec 2006 15:55:53 -0000 2, 27 -- Content-Disposition: inline 2, 27 -- Content-Type: text/plain; charset="iso-8859-1" 2, 27 -- MIME-Version: 1.0 2, 27 -- From: {geiger-at-vetigastropoda.com} 2, 27 -- To: vchristie-at-altransolutions.com, microscopy-at-microscopy.com 2, 27 -- Subject: Re: [Microscopy] viaWWW: Zeiss vs JEOL VPSEM 2, 27 -- Reply-To: geiger-at-vetigastropoda.com 2, 27 -- X-Origin: 69.229.108.153 2, 27 -- Date: Thu, 14 Dec 2006 07:55:53 -0800 2, 27 -- X-Uidl: 200612141347.kBEDl6Fu000837-at-ns.microscopy.com 2, 27 -- X-Mailer: AtMail 4.03 2, 27 -- X-Qmail-Scanner-Message-ID: {116611175392278416-at-st77.startlogic.com} 2, 27 -- Content-Transfer-Encoding: 8bit 2, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kBEG02sD014797 ==============================End of - Headers==============================
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: U of Id
Title-Subject: [Filtered] Cleaning a Lab6 emitter
Question: I am running a Denka Lab6 on an AMRAY 1830. We a lot of samples mounted in epoxy and the LaB6 now has a bad coating of what I think is epoxy residue (visible under a binoc). Does anyone have a suggestion on how to clean this safely?
You may use any organic solvent that is not corrosive to metals, Freon-113 or Chloroform are very good, tetrachloromethane is good, or any other chlorinated hydrocarbon if you can get any- these are usually restricted. Just soak the emitter, do not sonicate!
I dealt with a number oxidized LaB6 cathodes, when for example a user accidentally inserted specimen holder in the TEM without pre-pumping, while LaB6 was hot (some did so many times in row :-) Then vacuum system shots down, etc., and LaB6 catches some O2 from the air. All I do to cure that condition is to run LaB6 (for a day or so) at a slightly higher temperature at low KV and low emission to let poisoned layer of the crystal to evaporate. It usually works, but then there is a limit of how much abuse LaB6 can take.
However, I have doubts about the very cause of your LaB6 problem. What is the history of symptoms?
First, how did contamination got in the gun area? An SEM equipped for LaB6 use must be pumped differentially - the gun and column have dedicated IGP and are separated by differential aperture from the specimen chamber.
Second, I can hardly imagine organics condensing on an object heated to about 2000 degrees F.
History of the symptoms?
Vitaly Feingold SIA 2773 Heath Line Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com
----- Original Message ----- X-from: {tomw-at-uidaho.edu} To: {vitalylazar-at-att.net} Sent: Thursday, December 14, 2006 7:13 PM
I'm not sure about how to clean the LaB6 from Denka. But I have done this once with FEI cathodes.
What is puzzling is why you have to do this at all. The 1830 if set up for LaB6 has a 30L/m ion pump for the gun chamber. This should and does keep gun chamber vacuum really good. What IPG value are you currently reading? If you do not have a gun chamber ion pump, you cannot run LaB6. If you do have it, there is no obvious reason why the LaB6 would be contaminated. It makes no sense to me. I never had this happen. IPG was typically 40uA. Hopefully, your stand pipe valve is closed.
gary g.
At 04:13 PM 12/14/2006, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Thu Dec 14 19:55:14 2006 10, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kBF1tDLR029343 10, 20 -- for {microscopy-at-microscopy.com} ; Thu, 14 Dec 2006 19:55:13 -0600 10, 20 -- Received: (qmail 3838 invoked from network); 14 Dec 2006 17:50:37 -0800 10, 20 -- Received: by simscan 1.1.0 ppid: 3786, pid: 3833, t: 0.1689s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp4 with SMTP; 14 Dec 2006 17:50:36 -0800 10, 20 -- Message-Id: {7.0.1.0.2.20061214175110.02568880-at-gaugler.com} 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 10, 20 -- Date: Thu, 14 Dec 2006 17:55:08 -0800 10, 20 -- To: tomw-at-uidaho.edu 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] viaWWW: Cleaning a Lab6 emitter 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200612150013.kBF0DT36008436-at-ns.microscopy.com} 10, 20 -- References: {200612150013.kBF0DT36008436-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-20BE2D9D ==============================End of - Headers==============================
As we'll cecome next year a new TEM, we are studying the question to move all our microscopes in an other part of the building. But, if the place seems to have interesting side, obviously something must be wrong ! There is a power line which runs in a technical corridor in the underground, carrying some 800-1000 Amps for the supply of the whole building, and generating something like 50 mG in one of the possible room.
So, I have three questions :
First, is it possible to shield such a power line, to lower the field at it source (1" thick aluminum or cooper, or so) ? Is such a shielding technically possible, and not too expensive ? I think it would be better to try first to limit the perturbation at it source !
Secondly, what are the feedback from people working with dynamic magnetic field compensation ? How much do you have without it, and how does it work, with such a big field. Does it react fast enough, when the stray field changes.
Thirdly, a question for europeen lister, I'm looking for europeen manufactuerer of such dynamic compensation and/or static shielding. I know some, but I want to have several contacts.
Thanks in advance for help.
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
} Question: I am running a Denka Lab6 on an AMRAY 1830. We a } lot of samples mounted in epoxy and the LaB6 now has a bad } coating of what I think is epoxy residue (visible under a } binoc). Does anyone have a suggestion on how to clean this safely?
Are you referring to a residue on the Wehnelt or emitter? I have seen non-conductive residue deposited onto the Wehnelt by a LaB6 (as if LaB6 itself). It was relatively difficult to remove, but a weak acid solution helped (e.g., 10% HCl).
Genuinely, Michael Shaffer :o)
SEM/MLA Research Coordinator http://www.mun.ca/creait/maf/ Inco Innovation Centre Memorial University St. John's, NL
==============================Original Headers============================== 6, 20 -- From michael-at-shaffer.net Fri Dec 15 04:50:03 2006 6, 20 -- Received: from n034.sc1.cp.net (smtpout1448.sc1.he.tucows.com [64.97.157.148]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBFAo3jk029373 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 15 Dec 2006 04:50:03 -0600 6, 20 -- Received: from rarewolf (205.251.83.78) by n034.sc1.cp.net (7.2.069.1) (authenticated as michael-at-shaffer.net) 6, 20 -- id 457F403C00089034; Fri, 15 Dec 2006 10:49:56 +0000 6, 20 -- From: "michael shaffer" {michael-at-shaffer.net} 6, 20 -- To: {tomw-at-uidaho.edu} 6, 20 -- Cc: "MSA Microscopy list" {Microscopy-at-microscopy.com} 6, 20 -- Subject: RE: [Microscopy] viaWWW: Cleaning a Lab6 emitter 6, 20 -- Date: Fri, 15 Dec 2006 07:21:57 -0330 6, 20 -- Message-ID: {005301c72037$0b820930$4701a8c0-at-rarewolf} 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="us-ascii" 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- X-Mailer: Microsoft Office Outlook 11 6, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 6, 20 -- Thread-Index: Accf3W9d/0RRYqWPQwG+blCThWXrrAAWP7oA 6, 20 -- In-Reply-To: {200612150012.kBF0C8ZZ006213-at-ns.microscopy.com} ==============================End of - Headers==============================
Hello, I would not suggest to used 10% HCl for cleaning Whenelt assembly. 10% HCl solution might be too aggressive for highly polished stainless steel surface. In our lab, we routinely use ammonia solution. Is it also possible to use alcoholic solution of KOH. However, you can clean the stainless parts of Wehnelt, only. All brass components should be dismounted prior cleaning in ammonia or KOH solutions. Please, see the users guide for proper cleaning of your Wehnelt. If you would like, I can make a copy of cleaning procedure of Whenelt assembly from Philips CM100 users guide manual.
Best regards Oldrich
------------------------------------------ Oldrich Benada Institute of Microbiology, Acad. Sci. CR Videnska 1083 142 20 Praha 4 - Krc Czech Republic
On 15 Dec 2006 at 4:52, michael-at-shaffer.net wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Tom writes ... } } } Question: I am running a Denka Lab6 on an AMRAY 1830. We a } } lot of samples mounted in epoxy and the LaB6 now has a bad } } coating of what I think is epoxy residue (visible under a } } binoc). Does anyone have a suggestion on how to clean this safely? } } Are you referring to a residue on the Wehnelt or emitter? I have seen } non-conductive residue deposited onto the Wehnelt by a LaB6 (as if LaB6 } itself). It was relatively difficult to remove, but a weak acid solution } helped (e.g., 10% HCl). } } Genuinely, Michael Shaffer :o) } } SEM/MLA Research Coordinator } http://www.mun.ca/creait/maf/ } Inco Innovation Centre } Memorial University } St. John's, NL } } } ==============================Original Headers============================== } 6, 20 -- From michael-at-shaffer.net Fri Dec 15 04:50:03 2006 } 6, 20 -- Received: from n034.sc1.cp.net (smtpout1448.sc1.he.tucows.com [64.97.157.148]) } 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBFAo3jk029373 } 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 15 Dec 2006 04:50:03 -0600 } 6, 20 -- Received: from rarewolf (205.251.83.78) by n034.sc1.cp.net (7.2.069.1) (authenticated as michael-at-shaffer.net) } 6, 20 -- id 457F403C00089034; Fri, 15 Dec 2006 10:49:56 +0000 } 6, 20 -- From: "michael shaffer" {michael-at-shaffer.net} } 6, 20 -- To: {tomw-at-uidaho.edu} } 6, 20 -- Cc: "MSA Microscopy list" {Microscopy-at-microscopy.com} } 6, 20 -- Subject: RE: [Microscopy] viaWWW: Cleaning a Lab6 emitter } 6, 20 -- Date: Fri, 15 Dec 2006 07:21:57 -0330 } 6, 20 -- Message-ID: {005301c72037$0b820930$4701a8c0-at-rarewolf} } 6, 20 -- MIME-Version: 1.0 } 6, 20 -- Content-Type: text/plain; } 6, 20 -- charset="us-ascii" } 6, 20 -- Content-Transfer-Encoding: 7bit } 6, 20 -- X-Mailer: Microsoft Office Outlook 11 } 6, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 } 6, 20 -- Thread-Index: Accf3W9d/0RRYqWPQwG+blCThWXrrAAWP7oA } 6, 20 -- In-Reply-To: {200612150012.kBF0C8ZZ006213-at-ns.microscopy.com} } ==============================End of - Headers==============================
------------------------------------------ Oldrich Benada Mikrobiologický ústav AV CR Videnska 1083 142 20 Praha 4 - Krc
==============================Original Headers============================== 9, 24 -- From benada-at-biomed.cas.cz Fri Dec 15 05:39:05 2006 9, 24 -- Received: from biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBFBd4in009145 9, 24 -- for {microscopy-at-microscopy.com} ; Fri, 15 Dec 2006 05:39:04 -0600 9, 24 -- Received: from [147.231.44.101] (u117ob.mbu.cas.cz [147.231.44.101]) 9, 24 -- by biomed.cas.cz (8.13.4+Sun/8.13.3) with ESMTP id kBFBap7q017413; 9, 24 -- Fri, 15 Dec 2006 12:37:26 +0100 (CET) 9, 24 -- From: benada-at-biomed.cas.cz 9, 24 -- To: michael-at-shaffer.net, microscopy-at-microscopy.com 9, 24 -- Date: Fri, 15 Dec 2006 12:37:38 +0100 9, 24 -- MIME-Version: 1.0 9, 24 -- Subject: Re: [Microscopy] RE: viaWWW: Cleaning a Lab6 emitter 9, 24 -- Message-ID: {45829712.13334.E7C55F-at-benada.biomed.cas.cz} 9, 24 -- Return-receipt-to: benada-at-biomed.cas.cz 9, 24 -- Priority: normal 9, 24 -- In-reply-to: {200612151052.kBFAqgxb001492-at-ns.microscopy.com} 9, 24 -- References: {200612151052.kBFAqgxb001492-at-ns.microscopy.com} 9, 24 -- X-mailer: Pegasus Mail for Windows (4.41) 9, 24 -- Content-type: text/plain; charset=ISO-8859-2 9, 24 -- Content-description: Mail message body 9, 24 -- X-Antivirus: avast! (VPS 0659-0, 15.12.2006), Outbound message 9, 24 -- X-Antivirus-Status: Clean 9, 24 -- Content-Transfer-Encoding: 8bit 9, 24 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id kBFBd4in009145 ==============================End of - Headers==============================
Hello everyone, I routine clean my stainless steel Wehnelt with a little dilute NH3OH, but I'm removing tungsten, I'm not sure what you're clean off with a LaB6 filament.
But I would never use NH3OH or NaOH to clean brass parts. Most commercial brass and copper cleaners proudly exclaim "Does not contain ammonia." There is a reason for that. I sometime use an ammonia solution to strip copper fouling off steel, but I;m interested in cleaning the steel and not preserving the copper. I'm not saying you're wrong, but I would be very hesitant to clean brass in a basic solution.
Stay safe........ Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
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==============================Original Headers============================== 18, 18 -- From frank.karl-at-degussa.com Fri Dec 15 07:24:21 2006 18, 18 -- Received: from framailout1.rz.itson.com (mailout2.degussa.com [149.216.91.173]) 18, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBFDOKnR022563 18, 18 -- for {microscopy-at-msa.microscopy.com} ; Fri, 15 Dec 2006 07:24:21 -0600 18, 18 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [10.88.88.98]) 18, 18 -- by framailout1.rz.itson.com (8.13.4/8.13.4/Debian-3sarge3) with SMTP id kBFDOGoG004064 18, 18 -- for {microscopy-at-msa.microscopy.com} ; Fri, 15 Dec 2006 14:24:18 +0100 18, 18 -- In-Reply-To: {200612151140.kBFBeH0q011275-at-ns.microscopy.com} 18, 18 -- Subject: Re: [Microscopy] viaWWW: Cleaning a Lab6 emitter 18, 18 -- To: microscopy-at-msa.microscopy.com 18, 18 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 18, 18 -- Message-ID: {OFEF6D95E9.10C19786-ON86257245.00488E96-85257245.004996A5-at-degussa.com} 18, 18 -- From: frank.karl-at-degussa.com 18, 18 -- Date: Fri, 15 Dec 2006 08:23:48 -0500 18, 18 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 18, 18 -- 12/15/2006 07:24:19 AM 18, 18 -- MIME-Version: 1.0 18, 18 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
SEM/EDS has been important analytical instrumentation for the FBI for decades. We have recently expanded its utility in investigative roles, however, and therefore would like to ask the community for assistance. Whereas most SEM/EDS users need answers provided by quantitation and structural characterization, forensic inquiries generally necessitate “identifications†of questioned materials. This is generally possible only if the analyst is able to search his analytical results (spectra, primarily) against data from reference materials – as is the case with most other mature spectroscopies. We therefore have developed tools to archive and query the spectra and associated metadata of reference materials, and have produced a data set of thousands of materials in a variety of materials categories. Spectral and data queries against this database are consistently providing significant investigative direction. Of course quantitative analysis and other analytical techniques are additionally used where appropriate. Because the FBI has limited analytical capability and limited access to specific materials, I am appealing to you for either reference spectra that can be uploaded directly into our database, or materials that we can borrow and analyze at our facility (on a very limited, very specific basis). Literally all materials are viable candidates for inclusion – from commercial products to biologicals. Spectra must be exportable in EMSA format. Any data contributed will be restricted to FBI use only. If you would be willing to consider participating in this project, please contact me directly for details. We sincerely appreciate your consideration of this appeal!
Dennis Ward, FBI
Dennis Ward FBI Laboratory Chemistry Unit 2501 Investigation Parkway Quantico, VA 22135 v 703.632.7424
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The gun for the 1830 and associated components are compatible with Pol for cleaning and polishing. I used it for years on the gun, final apertures holder, anode aperture holder, etc.
Not sure about the LaB6 cathode however.
gary g.
At 05:27 AM 12/15/2006, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Fri Dec 15 11:32:57 2006 10, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kBFHWtMa017656 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 15 Dec 2006 11:32:56 -0600 10, 20 -- Received: (qmail 12969 invoked from network); 15 Dec 2006 09:28:18 -0800 10, 20 -- Received: by simscan 1.1.0 ppid: 12911, pid: 12967, t: 0.1692s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp4 with SMTP; 15 Dec 2006 09:28:17 -0800 10, 20 -- Message-Id: {7.0.1.0.2.20061215093045.02561ec0-at-gaugler.com} 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 10, 20 -- Date: Fri, 15 Dec 2006 09:32:50 -0800 10, 20 -- To: frank.karl-at-degussa.com 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] Re: viaWWW: Cleaning a Lab6 emitter 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200612151327.kBFDRlpq025527-at-ns.microscopy.com} 10, 20 -- References: {200612151327.kBFDRlpq025527-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-7F1D5C44 ==============================End of - Headers==============================
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JEOL use their systems regulary in the UK for TEM and FE-SEM installations. In simple cases, we install ourselves, for more complex situations with multiple microscopes and changing fields, we get them to come in and design an appropriate set of coils.
Cancellation is not instantaneous - can take 1-2 seconds. So, if fields are fairly stable and only change occassionally/slowly, it can probably be cancelled, always depending on how sensitive the TEM is. If you have rapidly changing fields, then it gets more difficult and tends to be less sucessful.
Best regards, -- Larry Stoter JEOL (UK) Ltd tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoluk.com
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==============================Original Headers============================== 6, 18 -- From larry-at-celtic.freewire.co.uk Fri Dec 15 11:40:08 2006 6, 18 -- Received: from get.freewire.net (get.freewire.net [86.54.106.202] (may be forged)) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBFHe8ST028543 6, 18 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 15 Dec 2006 11:40:08 -0600 6, 18 -- Received: from [194.164.78.187] (modem-rack4-modem187.netkonect.net [194.164.78.187]) 6, 18 -- by get.freewire.net (8.13.6/8.11.6) with ESMTP id kBFHe8mZ014452; 6, 18 -- Fri, 15 Dec 2006 17:40:09 GMT 6, 18 -- Mime-Version: 1.0 6, 18 -- Message-Id: {p06210200c1a88b415e82-at-[194.164.78.235]} 6, 18 -- In-Reply-To: {200612150815.kBF8FnX9025522-at-ns.microscopy.com} 6, 18 -- References: {200612150815.kBF8FnX9025522-at-ns.microscopy.com} 6, 18 -- Date: Fri, 15 Dec 2006 17:32:51 +0000 6, 18 -- To: jacques.faerber-at-ipcms.u-strasbg.fr, Microscopy-at-MSA.Microscopy.Com 6, 18 -- From: Larry Stoter {larry-at-celtic.freewire.co.uk} 6, 18 -- Subject: Re: [Microscopy] power line and stray field shielding 6, 18 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 6, 18 -- Content-Transfer-Encoding: 8bit 6, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kBFHe8ST028543 ==============================End of - Headers==============================
Tenure-track faculty vacancy in the Department of Materials Science and Engineering at Lehigh University.
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Details may be found at: http://www.lehigh.edu/~inmatsci/research/bio_search.html
For questions regarding the application process, contact the search coordinator: Sharon Coe at slc6-at-lehigh.edu.
........... Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
==============================Original Headers============================== 8, 21 -- From jae5-at-lehigh.edu Fri Dec 15 13:57:40 2006 8, 21 -- Received: from rain.CC.Lehigh.EDU (rain.CC.Lehigh.EDU [128.180.39.20]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBFJvdZF010748 8, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 15 Dec 2006 13:57:40 -0600 8, 21 -- Received: from [127.0.0.1] (Dyn055141.mat.Lehigh.EDU [128.180.55.141]) 8, 21 -- (authenticated bits=0) 8, 21 -- by rain.CC.Lehigh.EDU (8.13.8/8.13.8) with ESMTP id kBFJvX6I032405 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 15 Dec 2006 14:57:38 -0500 8, 21 -- Message-ID: {4582FE2D.3030804-at-lehigh.edu} 8, 21 -- Date: Fri, 15 Dec 2006 14:57:33 -0500 8, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu} 8, 21 -- Organization: Lehigh University 8, 21 -- User-Agent: Thunderbird 1.5.0.8 (Windows/20061025) 8, 21 -- MIME-Version: 1.0 8, 21 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com} 8, 21 -- Subject: Faculty Vacancy in Biomaterials 8, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 21 -- Content-Transfer-Encoding: 7bit 8, 21 -- X-Virus-Scanned: ClamAV version 0.88.7, clamav-milter version 0.88.7 on rain.CC.Lehigh.EDU 8, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
There are several suggestions for cleaning the interior parts of electron microscopes in Section 2.10.4c (pp. 71-74) of my book Vacuum Methods in Electron Microscopy (available from SPI Supplies, Ladd, M. E, Taylor, etc.) that might be useful in solving the present problem. Specifically, mentioned there is a recommendation from Peter B. Sewell of LAB-6 Inc. for removing LaB6 deposits by soaking for about a minute in a solution consisting of one part concentrated hydrochloric acid and 4 parts water.
Good luck, and Happy Holidays Wil Bigelow -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-engin.umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 2, 14 -- From bigelow-at-engin.umich.edu Fri Dec 15 14:58:47 2006 2, 14 -- Received: from srvr22.engin.umich.edu (srvr22.engin.umich.edu [141.213.75.21]) 2, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBFKwkVA023089 2, 14 -- for {microscopy-at-microscopy.com} ; Fri, 15 Dec 2006 14:58:47 -0600 2, 14 -- Received: from [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 2, 14 -- by srvr22.engin.umich.edu (8.13.6/8.13.6) with ESMTP id kBFKwi1c004942 2, 14 -- for {microscopy-at-microscopy.com} ; Fri, 15 Dec 2006 15:58:44 -0500 (EST) 2, 14 -- Mime-Version: 1.0 2, 14 -- Message-Id: {p06210203c1a8ba741ae0-at-[141.212.131.221]} 2, 14 -- Date: Fri, 15 Dec 2006 15:58:43 -0500 2, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 2, 14 -- From: Wil Bigelow {bigelow-at-engin.umich.edu} 2, 14 -- Subject: [Microscopy]RE: Cleaning LaB6 guns 2, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
50 mG tells me that you have a mis-wiring issue or a grounding of the neutral, or an unintentional grounding to metal to begin with. You should do an EMF survey (grid style) then hunt down the wiring problems. This should drop it to under 5 mG, likely under 3 mG.
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC PO Box 34140 Indianapolis, IN 46234 (317) 752-6386 off (317) 328-9594 fax (317) 409-3238 cell
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-----Original Message----- X-from: jacques.faerber-at-ipcms.u-strasbg.fr [mailto:jacques.faerber-at-ipcms.u-strasbg.fr] Sent: Friday, December 15, 2006 3:15 AM To: ph2-at-sprynet.com
Hi all
As we'll cecome next year a new TEM, we are studying the question to move all our microscopes in an other part of the building. But, if the place seems to have interesting side, obviously something must be wrong ! There is a power line which runs in a technical corridor in the underground, carrying some 800-1000 Amps for the supply of the whole building, and generating something like 50 mG in one of the possible room.
So, I have three questions :
First, is it possible to shield such a power line, to lower the field at it source (1" thick aluminum or cooper, or so) ? Is such a shielding technically possible, and not too expensive ? I think it would be better to try first to limit the perturbation at it source !
Secondly, what are the feedback from people working with dynamic magnetic field compensation ? How much do you have without it, and how does it work, with such a big field. Does it react fast enough, when the stray field changes.
Thirdly, a question for europeen lister, I'm looking for europeen manufactuerer of such dynamic compensation and/or static shielding. I know some, but I want to have several contacts.
Thanks in advance for help.
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
} First, is it possible to shield such a power line, to lower the } field at it source (1" thick aluminum or cooper, or so) ? Is such a } shielding technically possible, and not too expensive ? I think it would } be better to try first to limit the perturbation at it source ! }
Hi Larry,
I can only address the shielding... You can employ shielding if physically practical, but do not use Al or Cu. The conductors must be totally enclosed for best result. Annealed iron/steel is good. It does not have to be extremely thick. Best is a material called Mu-metal (a hydrogen annealed Ni/Fe alloy if memory serves. Mu-metal is a bit pricy, however, and for best results work-hardening during fabrication should be minimized.
Regards, Woody White BWXT Services
==============================Original Headers============================== 7, 27 -- From nwwhite-at-bwxt.com Mon Dec 18 07:07:25 2006 7, 27 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBID7Puk004511 7, 27 -- for {microscopy-at-msa.microscopy.com} ; Mon, 18 Dec 2006 07:07:25 -0600 7, 27 -- Received: from ([131.184.13.224]) 7, 27 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.3265256; 7, 27 -- Mon, 18 Dec 2006 08:07:03 -0500 7, 27 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); 7, 27 -- Mon, 18 Dec 2006 08:07:03 -0500 7, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 27 -- Content-class: urn:content-classes:message 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="us-ascii" 7, 27 -- Subject: RE: [Microscopy] Re: power line and stray field shielding 7, 27 -- Date: Mon, 18 Dec 2006 08:07:03 -0500 7, 27 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB703D0EF-at-BWXSPO01.BWXS.BWXTECH.NET} 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- Thread-Topic: [Microscopy] Re: power line and stray field shielding 7, 27 -- Thread-Index: AccgcCTK/AfNKel3R3StVZENGPeGGwCNFoyA 7, 27 -- From: "White, Woody N." {NWWhite-at-bwxt.com} 7, 27 -- To: {larry-at-celtic.freewire.co.uk} , 7, 27 -- "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 7, 27 -- X-OriginalArrivalTime: 18 Dec 2006 13:07:03.0682 (UTC) FILETIME=[69698620:01C722A5] 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kBID7Puk004511 ==============================End of - Headers==============================
I am hoping that someone could provide schematics for a Hitachi S-530 SEM, particularly for the vacuum controller. I'm helping a college revive an old instrument and don't have the documents for it. Presently stuck on the vacuum controller not completing a pumpdown sequence.
Allen R. Sampson, Owner Advanced Research Systems 317 North 4th. Street Saint Charles, Illinois 60174 phone (630) 513-7093 fax (630) 513-7092 email mailto:ars-at-sem.com web www.sem.com
==============================Original Headers============================== 5, 20 -- From ars-at-sem.com Tue Dec 19 03:11:00 2006 5, 20 -- Received: from mail.sem.com (ns2.sem.com [66.167.158.194]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBJ9B05f013031 5, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 19 Dec 2006 03:11:00 -0600 5, 20 -- Received: from VaioD (mars.sem.com [66.73.253.98]) 5, 20 -- by mail.sem.com (8.13.1/8.12.6) with SMTP id kBJ9PPKF042442 5, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 19 Dec 2006 03:25:25 -0600 (CST) 5, 20 -- (envelope-from ars-at-sem.com) 5, 20 -- Received: by localhost with Microsoft MAPI; Tue, 19 Dec 2006 03:11:22 -0600 5, 20 -- Message-ID: {01C7231B.5C779B20.ars-at-sem.com} 5, 20 -- From: "Allen R. Sampson" {ars-at-sem.com} 5, 20 -- Reply-To: "ars-at-sem.com" {ars-at-sem.com} 5, 20 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 5, 20 -- Subject: SEM seeking Hitachi S-530 schematics 5, 20 -- Date: Tue, 19 Dec 2006 03:11:21 -0600 5, 20 -- Organization: Advanced Research Systems 5, 20 -- X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; charset="us-ascii" 5, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I use Adobe Photoshop CS (v9) under Windows 2000. Yesterday, I encountered a problem when saving (as) files. Normally I selected the file type to save as, then typed in only the filename. PS would add the selected file extension (typically .TIF) as a default.
Now, if I do that, PS will save the file fine, but adds no file extention (e.g. .TIF). I have restarted the PC as well as reinstalled PS (installer-repair) and the problem persists. I tried selecting .JPG as the file type and the same symptom presented. ...No .JPG extension, only the filename. This is not a "viewing" problem. The file types are not hidden. Also, if I edit the filename and add .TIF to a previously saved file, all is well.
I have tried Adobe support to no avail. Their suggestion was to manually type in filename.TIF. This does work as long as what you type matches the actual file type, but I would feel much better to understand what is happening and make it right.
Suggestions?
Thanks, Woody White BWXT Services
==============================Original Headers============================== 8, 26 -- From nwwhite-at-bwxt.com Tue Dec 19 10:17:04 2006 8, 26 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBJGH3wa002967 8, 26 -- for {microscopy-at-msa.microscopy.com} ; Tue, 19 Dec 2006 10:17:03 -0600 8, 26 -- Received: from ([131.184.13.224]) 8, 26 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.3299847; 8, 26 -- Tue, 19 Dec 2006 11:16:49 -0500 8, 26 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); 8, 26 -- Tue, 19 Dec 2006 11:16:50 -0500 8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 26 -- Content-class: urn:content-classes:message 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-Type: text/plain; 8, 26 -- charset="us-ascii" 8, 26 -- Subject: Photoshop glitch - Opinions? 8, 26 -- Date: Tue, 19 Dec 2006 11:16:49 -0500 8, 26 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB703D0F3-at-BWXSPO01.BWXS.BWXTECH.NET} 8, 26 -- X-MS-Has-Attach: 8, 26 -- X-MS-TNEF-Correlator: 8, 26 -- Thread-Topic: Photoshop glitch - Opinions? 8, 26 -- Thread-Index: AccjiRaz9NHYiUO/QLKut+jTCbxnPA== 8, 26 -- From: "White, Woody N." {NWWhite-at-bwxt.com} 8, 26 -- To: "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 8, 26 -- X-OriginalArrivalTime: 19 Dec 2006 16:16:50.0229 (UTC) FILETIME=[16BC7650:01C72389] 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kBJGH3wa002967 ==============================End of - Headers==============================
The problem may be spyware. Try running anti-spyware programs like Spybot Search and Destroy and also LavaSoft Ad-Aware SE. Both programs are free. No single program seems to find all spyware/adware, so by running a couple one will catch what the other misses. If that doesn't solve the issue, then read on.
Software that is installed on a computer will develop errors over time where a zero stored in memory is replaced by a one or visa versa. It may happen when a cosmic ray particle strikes a bit thereby changing it's value or by some other random process. Over time a number of these errors will accumulate and glitches develop. This is sometimes called "bit rot". The best solution is to back up all your data, format the hard drive, and perform a fresh installation of Windows (or OSx for Mac). Then continue installing Photoshop and any other programs you use. Bit rot happens to all programs. The bigger and more complex ones have greater opportunity for errors to occur and so are more likely to develop glitches. Some computer gurus recommend a fresh installation of Windows at six month or one year intervals. Bit rot is another good reason for frequent data backups too.
Take care,
Bob Carter 2000 Bayshore Road Lopez Island, WA 98261-8595
Original Message ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hello All,
I use Adobe Photoshop CS (v9) under Windows 2000. Yesterday, I encountered a problem when saving (as) files. Normally I selected the file type to save as, then typed in only the filename. PS would add the selected file extension (typically .TIF) as a default.
Now, if I do that, PS will save the file fine, but adds no file extention (e.g. .TIF). I have restarted the PC as well as reinstalled PS (installer-repair) and the problem persists. I tried selecting .JPG as the file type and the same symptom presented. ...No .JPG extension, only the filename. This is not a "viewing" problem. The file types are not hidden. Also, if I edit the filename and add .TIF to a previously saved file, all is well.
I have tried Adobe support to no avail. Their suggestion was to manually type in filename.TIF. This does work as long as what you type matches the actual file type, but I would feel much better to understand what is happening and make it right.
Suggestions?
Thanks, Woody White BWXT Services
==============================Original Headers============================== 8, 26 -- From nwwhite-at-bwxt.com Tue Dec 19 10:17:04 2006 8, 26 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBJGH3wa002967 8, 26 -- for {microscopy-at-msa.microscopy.com} ; Tue, 19 Dec 2006 10:17:03 -0600 8, 26 -- Received: from ([131.184.13.224]) 8, 26 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.3299847; 8, 26 -- Tue, 19 Dec 2006 11:16:49 -0500 8, 26 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); 8, 26 -- Tue, 19 Dec 2006 11:16:50 -0500 8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 26 -- Content-class: urn:content-classes:message 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-Type: text/plain; 8, 26 -- charset="us-ascii" 8, 26 -- Subject: Photoshop glitch - Opinions? 8, 26 -- Date: Tue, 19 Dec 2006 11:16:49 -0500 8, 26 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB703D0F3-at-BWXSPO01.BWXS.BWXTECH.NET} 8, 26 -- X-MS-Has-Attach: 8, 26 -- X-MS-TNEF-Correlator: 8, 26 -- Thread-Topic: Photoshop glitch - Opinions? 8, 26 -- Thread-Index: AccjiRaz9NHYiUO/QLKut+jTCbxnPA== 8, 26 -- From: "White, Woody N." {NWWhite-at-bwxt.com} 8, 26 -- To: "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 8, 26 -- X-OriginalArrivalTime: 19 Dec 2006 16:16:50.0229 (UTC) FILETIME=[16BC7650:01C72389] 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kBJGH3wa002967 ==============================End of - Headers==============================
-- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.432 / Virus Database: 268.15.24/592 - Release Date: 12/18/2006 1:45 PM
==============================Original Headers============================== 20, 32 -- From bob-at-rockisland.com Tue Dec 19 11:41:09 2006 20, 32 -- Received: from mars.rockisland.com (dnscache2.rockisland.com [64.119.0.12]) 20, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBJHf9bW016186 20, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 19 Dec 2006 11:41:09 -0600 20, 32 -- Received: from localhost (localhost [127.0.0.1]) 20, 32 -- by localhost.rockisland.com (Postfix) with ESMTP id 0080D5976; 20, 32 -- Tue, 19 Dec 2006 09:41:08 -0800 (PST) 20, 32 -- (envelope-from bob-at-rockisland.com) 20, 32 -- X-Virus-Scanned: amavisd-new at rockisland.com 20, 32 -- Received: from mars.rockisland.com ([127.0.0.1]) 20, 32 -- by localhost (smtp.rockisland.com [127.0.0.1]) (amavisd-new, port 10024) 20, 32 -- with ESMTP id YFguJksJEHPG; Tue, 19 Dec 2006 09:41:08 -0800 (PST) 20, 32 -- Received: from housecat (adsl-sj-11-138.rockisland.net [64.119.11.138]) 20, 32 -- by mars.rockisland.com (Postfix) with SMTP id 6746E589B; 20, 32 -- Tue, 19 Dec 2006 09:41:08 -0800 (PST) 20, 32 -- (envelope-from bob-at-rockisland.com) 20, 32 -- Message-ID: {001001c72394$de2a8910$0302a8c0-at-housecat} 20, 32 -- From: "Bob Carter" {bob-at-rockisland.com} 20, 32 -- To: {Microscopy-at-microscopy.com} 20, 32 -- Cc: {NWWhite-at-bwxt.com} 20, 32 -- Subject: Photoshop glitch 20, 32 -- Date: Tue, 19 Dec 2006 09:41:08 -0800 20, 32 -- MIME-Version: 1.0 20, 32 -- Content-Type: text/plain; 20, 32 -- format=flowed; 20, 32 -- charset="iso-8859-1"; 20, 32 -- reply-type=original 20, 32 -- Content-Transfer-Encoding: 7bit 20, 32 -- X-Priority: 3 20, 32 -- X-MSMail-Priority: Normal 20, 32 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3028 20, 32 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 ==============================End of - Headers==============================
} I use Adobe Photoshop CS (v9) under Windows 2000. Yesterday, } I encountered a problem when saving (as) files. Normally I } selected the file type to save as, then typed in only the } filename. PS would add the selected file extension } (typically .TIF) as a default. } } Now, if I do that, PS will save the file fine, but adds no } file extention (e.g. .TIF). ...
Always your best bet for Photoshop issues is the sheer number of users who habit the Adobe forums ...
http://www.adobeforums.com
Registration is required, but it's a worthwhile resource of information.
cheerios :o) michael shaffer
SEM/MLA Research Coordinator {http://www.mun.ca/creait/maf/} Inco Innovation Centre Memorial University St. John's, Newfoundland
==============================Original Headers============================== 9, 20 -- From michael-at-shaffer.net Tue Dec 19 11:53:41 2006 9, 20 -- Received: from n034.sc1.cp.net (smtpout1471.sc1.he.tucows.com [64.97.157.171]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBJHrfCZ027344 9, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 19 Dec 2006 11:53:41 -0600 9, 20 -- Received: from roamingwolf (134.153.130.141) by n034.sc1.cp.net (7.2.069.1) (authenticated as Michael-at-Shaffer.net) 9, 20 -- id 4586853D00045DF2; Tue, 19 Dec 2006 17:52:26 +0000 9, 20 -- From: "michael shaffer" {michael-at-shaffer.net} 9, 20 -- To: {NWWhite-at-bwxt.com} 9, 20 -- Cc: "MSA Microscopy list" {Microscopy-at-microscopy.com} 9, 20 -- Subject: RE: [Microscopy] Photoshop glitch - Opinions? 9, 20 -- Date: Tue, 19 Dec 2006 14:22:24 -0330 9, 20 -- Message-ID: {001601c72396$71b3e4f0$8d829986-at-roamingwolf} 9, 20 -- MIME-Version: 1.0 9, 20 -- Content-Type: text/plain; 9, 20 -- charset="us-ascii" 9, 20 -- Content-Transfer-Encoding: 7bit 9, 20 -- X-Mailer: Microsoft Office Outlook 11 9, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 9, 20 -- Thread-Index: AccjiRYApncSYdNFSACOgwf3TaHIIwADNkog 9, 20 -- In-Reply-To: {200612191617.kBJGHw1t003710-at-ns.microscopy.com} ==============================End of - Headers==============================
Thanks for all the suggestions. When I have the chance, I will completely remove PS entirely and re-install fresh instead of an installer "repair". This is a pain since I have 3 upgrades - Have to start with 5.0 and work my way up :(
However, no fix yet. Was hoping the preference box had somehow un-checked itself. But in looking, I find the only choice given is upper or lower case, not whether or not to use an extension. ...Unless that choice is accidentally missing - see below.
Jim... I feel you pain! Several times when I have gone to edit} preferences} file handling, the window pops up with all the buttons there - but no text defining them! ...Resembles your problem. If I go to another preference, then return to the file window, the buttons *and* text are then displayed. Weird!
Will try the user group Michael suggested ASAP, but with the holidays approaching, may have to back-burner the problem and meet some customer deadlines. ...I am getting samples by the wheelbarrow load ;)
Hope you all have an enjoyable holiday.
Woody
==============================Original Headers============================== 9, 26 -- From nwwhite-at-bwxt.com Tue Dec 19 12:17:08 2006 9, 26 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBJIH8dR006212 9, 26 -- for {microscopy-at-msa.microscopy.com} ; Tue, 19 Dec 2006 12:17:08 -0600 9, 26 -- Received: from ([131.184.13.224]) 9, 26 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.3301690; 9, 26 -- Tue, 19 Dec 2006 13:16:45 -0500 9, 26 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); 9, 26 -- Tue, 19 Dec 2006 13:16:45 -0500 9, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 26 -- Content-class: urn:content-classes:message 9, 26 -- MIME-Version: 1.0 9, 26 -- Content-Type: text/plain; 9, 26 -- charset="us-ascii" 9, 26 -- Subject: RE: [Microscopy] Photoshop glitch - Opinions? 9, 26 -- Date: Tue, 19 Dec 2006 13:16:44 -0500 9, 26 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB703D0F4-at-BWXSPO01.BWXS.BWXTECH.NET} 9, 26 -- X-MS-Has-Attach: 9, 26 -- X-MS-TNEF-Correlator: 9, 26 -- Thread-Topic: [Microscopy] Photoshop glitch - Opinions? 9, 26 -- Thread-Index: Accji7D8AEEDX6wsR52QuX2QpZURFgACvHmw 9, 26 -- From: "White, Woody N." {NWWhite-at-bwxt.com} 9, 26 -- To: "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 9, 26 -- X-OriginalArrivalTime: 19 Dec 2006 18:16:45.0355 (UTC) FILETIME=[D75D8FB0:01C72399] 9, 26 -- Content-Transfer-Encoding: 8bit 9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kBJIH8dR006212 ==============================End of - Headers==============================
Many (if not most) computers today employ ECC or other types of parity checking memory. In these cases, bit level errors in memory are automatically corrected for.
I also have to say that while I've certainly experienced problems I could trace to malware or hard drive failures, I've never see never anything that I could attribute to so called "bit rot". john
At 09:47 AM 12/19/2006, you wrote: } Software that is installed on a computer will develop errors over time } where a zero stored in memory is replaced by a one or visa versa. It may } happen when a cosmic ray particle strikes a bit thereby changing it's value } or by some other random process. Over time a number of these errors will } accumulate and glitches develop. This is sometimes called "bit rot". The } best solution is to back up all your data, format the hard drive, and } perform a fresh installation of Windows (or OSx for Mac). Then continue } installing Photoshop and any other programs you use. Bit rot happens to all } programs. The bigger and more complex ones have greater opportunity for } errors to occur and so are more likely to develop glitches. Some computer } gurus recommend a fresh installation of Windows at six month or one year } intervals. Bit rot is another good reason for frequent data backups too. } } Take care, } } Bob Carter } 2000 Bayshore Road } Lopez Island, WA 98261-8595
John J. Donovan donovan-at-uoregon.edu University of Oregon (541) 346-4632 (office) 1260 Franklin Blvd (541) 346-4655 (probe) Eugene, OR (541) 346-4778 (SEM) 97403-1272 (541) 346-4692 (FAX)
Lab Web: http://epmalab.uoregon.edu/
==============================Original Headers============================== 6, 21 -- From donovan-at-uoregon.edu Tue Dec 19 13:39:07 2006 6, 21 -- Received: from smtp.uoregon.edu (mserv4.uoregon.edu [128.223.142.54]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBJJd7BX019221 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 19 Dec 2006 13:39:07 -0600 6, 21 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.92.75]) 6, 21 -- (authenticated bits=0) 6, 21 -- by smtp.uoregon.edu (8.13.7/8.13.7) with ESMTP id kBJJd6GB015528 6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 19 Dec 2006 11:39:06 -0800 6, 21 -- Message-Id: {7.0.1.0.0.20061219113100.03a03d20-at-uoregon.edu} 6, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 6, 21 -- Date: Tue, 19 Dec 2006 11:36:31 -0800 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- From: John Donovan {donovan-at-uoregon.edu} 6, 21 -- Subject: Re: [Microscopy] Photoshop glitch 6, 21 -- In-Reply-To: {200612191747.kBJHl1Tv025837-at-ns.microscopy.com} 6, 21 -- References: {200612191747.kBJHl1Tv025837-at-ns.microscopy.com} 6, 21 -- Mime-Version: 1.0 6, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 21 -- X-Virus-Scanned: ClamAV 0.88.7/2358/Tue Dec 19 08:15:46 2006 on mserv4 6, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Photoshop 9 aka CS2 DOES have a preference for whether or not to add the extension. In the file-handling preferences it is Append File Extension: Always/Never/Ask. Probably what has happened is simply that your preferences file has gotten corrupted. So throw it into the trash and let Photoshop create a new one when you next run it. Chances are that will fix your problem.
On Dec 19, 2006, at 1:17 PM, NWWhite-at-bwxt.com wrote:
} However, no fix yet. Was hoping the preference box had somehow } un-checked itself. But in looking, I find the only choice given is } upper } or lower case, not whether or not to use an extension. ...Unless that } choice is accidentally missing - see below.
John Russ
==============================Original Headers============================== 8, 21 -- From DrJohnRuss-at-aol.com Tue Dec 19 13:45:22 2006 8, 21 -- Received: from imo-m22.mail.aol.com (imo-m22.mx.aol.com [64.12.137.3]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBJJjMDY028450 8, 21 -- for {microscopy-at-msa.microscopy.com} ; Tue, 19 Dec 2006 13:45:22 -0600 8, 21 -- Received: from DrJohnRuss-at-aol.com 8, 21 -- by imo-m22.mx.aol.com (mail_out_v38_r7.6.) id 3.d0b.474f939 (49383); 8, 21 -- Tue, 19 Dec 2006 14:45:17 -0500 (EST) 8, 21 -- Received: from [192.168.123.187] (cpe-065-190-140-239.nc.res.rr.com [65.190.140.239]) by ciaaol-d06.mail.aol.com (v114.2) with ESMTP id MAILCIAAOLD065-c0e74588414a38d; Tue, 19 Dec 2006 14:45:15 -0500 8, 21 -- Mime-Version: 1.0 (Apple Message framework v752.2) 8, 21 -- In-Reply-To: {200612191817.kBJIHVG4006916-at-ns.microscopy.com} 8, 21 -- References: {200612191817.kBJIHVG4006916-at-ns.microscopy.com} 8, 21 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 8, 21 -- Message-Id: {60EFF99A-F17F-4BF6-9C37-5BDA0EF5F478-at-AOL.com} 8, 21 -- Content-Transfer-Encoding: 7bit 8, 21 -- From: John Russ {DrJohnRuss-at-aol.com} 8, 21 -- Subject: Re: [Microscopy] RE: Photoshop glitch - Opinions? 8, 21 -- Date: Tue, 19 Dec 2006 14:45:11 -0500 8, 21 -- To: NWWhite-at-bwxt.com, microscopy-at-msa.microscopy.com 8, 21 -- X-Mailer: Apple Mail (2.752.2) 8, 21 -- X-AOL-IP: 65.190.140.239 8, 21 -- X-Spam-Flag: NO ==============================End of - Headers==============================
I have the schematics for the S-520 SEM evacuating sequence. Is that what you're after?
John Brealey Queen Elizabeth Hospital EM Unit Adelaide, South Australia
Hi all.
I am hoping that someone could provide schematics for a Hitachi S-530 SEM, particularly for the vacuum controller. I'm helping a college revive an old instrument and don't have the documents for it. Presently stuck on the vacuum controller not completing a pumpdown sequence.
Allen R. Sampson, Owner Advanced Research Systems 317 North 4th. Street Saint Charles, Illinois 60174 phone (630) 513-7093 fax (630) 513-7092 email mailto:ars-at-sem.com web www.sem.com
==============================Original Headers============================== 13, 33 -- From john.brealey-at-imvs.sa.gov.au Tue Dec 19 17:21:31 2006 13, 33 -- Received: from adl0741.systems.sa.gov.au (adl0741.systems.sa.gov.au [143.216.236.20]) 13, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBJNLTv6013816 13, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 19 Dec 2006 17:21:30 -0600 13, 33 -- Received: from adl0741.systems.sa.gov.au (localhost [127.0.0.1]) 13, 33 -- by adl0741.systems.sa.gov.au OUTGOING (8.12.10/8.12.10) with ESMTP id kBJNLSPb002066 13, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 20 Dec 2006 09:51:28 +1030 (CST)' 13, 33 -- Received: from ablett.imvs.sa.gov.au (ablett.imvs.sa.gov.au [10.20.98.41]) 13, 33 -- by adl0741.systems.sa.gov.au INCOMING (8.12.10/8.12.10) with ESMTP id kBJNLSvW002061 13, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 20 Dec 2006 09:51:28 +1030 (CST)' 13, 33 -- Received: from localhost (mesh.imvs.sa.gov.au [10.20.98.37]) 13, 33 -- by ablett.imvs.sa.gov.au (Postfix) with ESMTP id 327C334BC0 13, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 20 Dec 2006 09:51:26 +1030 (CST) 13, 33 -- X-Virus-Scanned: by amavisd-new at imvs.sa.gov.au 13, 33 -- Received: from ablett.imvs.sa.gov.au ([10.20.98.41]) 13, 33 -- by localhost (.imvs.sa.gov.au [10.20.98.37]) (amavisd-new, port 10024) 13, 33 -- with LMTP id 6Nezsvk6VH0k for {Microscopy-at-MSA.Microscopy.Com} ; 13, 33 -- Wed, 20 Dec 2006 09:51:19 +1030 (CST) 13, 33 -- Received: from iqe36042 (iqepc200.imvs.sa.gov.au [10.20.138.200]) 13, 33 -- by ablett.imvs.sa.gov.au (Postfix) with SMTP id 7642B34BBC 13, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 20 Dec 2006 09:51:11 +1030 (CST) 13, 33 -- From: "John Brealey" {john.brealey-at-imvs.sa.gov.au} 13, 33 -- To: "Listserver" {Microscopy-at-MSA.Microscopy.Com} 13, 33 -- Subject: SEM Schematics 13, 33 -- Date: Wed, 20 Dec 2006 09:51:09 +1030 13, 33 -- Message-ID: {000601c723c4$5e3fcc30$c88a140a-at-iqe36042} 13, 33 -- MIME-Version: 1.0 13, 33 -- Content-Type: text/plain; 13, 33 -- charset="us-ascii" 13, 33 -- Content-Transfer-Encoding: 7bit 13, 33 -- X-Mailer: Microsoft Office Outlook 11 13, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1807 13, 33 -- Thread-Index: AccjxFFN34uPeaL4Qn6oMFIuzqfcPg== ==============================End of - Headers==============================
Hi, I have a failure analysis problem looking at GaAs devices. It seems to me that the best way of looking for the failure would be to make a relatively thick (~1um) plan TEM section which includes the appropriate parts of the device structure. However I have the feeling that my 200 kV JEOL 2011 may not have enough oomph to get clear images. Does anyone know of a higher voltage TEM in the UK which I could hire for a day or two? Relatively low mag imaging would be good too since these devices are tens of microns wide.
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==============================Original Headers============================== 9, 31 -- From richard.beanland-at-bookham.com Wed Dec 20 04:10:30 2006 9, 31 -- Received: from mail78.messagelabs.com (mail78.messagelabs.com [195.245.230.131]) 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id kBKAATOk006973 9, 31 -- for {microscopy-at-microscopy.com} ; Wed, 20 Dec 2006 04:10:29 -0600 9, 31 -- X-VirusChecked: Checked 9, 31 -- X-Env-Sender: richard.beanland-at-bookham.com 9, 31 -- X-Msg-Ref: server-7.tower-78.messagelabs.com!1166609427!47143150!1 9, 31 -- X-StarScan-Version: 5.5.10.7; banners=bookham.com,-,- 9, 31 -- X-Originating-IP: [213.249.209.179] 9, 31 -- Received: (qmail 8429 invoked from network); 20 Dec 2006 10:10:27 -0000 9, 31 -- Received: from unknown (HELO cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 9, 31 -- by server-7.tower-78.messagelabs.com with SMTP; 20 Dec 2006 10:10:27 -0000 9, 31 -- Received: from cas-smx-02.BOOKHAM.ENTERPRISE.PRI ([10.4.0.223]) by cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI with Microsoft SMTPSVC(6.0.3790.1830); 9, 31 -- Wed, 20 Dec 2006 10:10:50 +0000 9, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 31 -- Content-class: urn:content-classes:message 9, 31 -- MIME-Version: 1.0 9, 31 -- Content-Type: text/plain; 9, 31 -- charset="us-ascii" 9, 31 -- Subject: High voltage TEM in the UK 9, 31 -- Date: Wed, 20 Dec 2006 10:10:49 -0000 9, 31 -- Message-ID: {9645D3E33E4C6548B12A7B25F611533E2CCAAD-at-cas-smx-02.BOOKHAM.ENTERPRISE.PRI} 9, 31 -- X-MS-Has-Attach: 9, 31 -- X-MS-TNEF-Correlator: 9, 31 -- Thread-Topic: High voltage TEM in the UK 9, 31 -- thread-index: AcckHx9QMKNYlHg8RG6IQfpT5nHBBA== 9, 31 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 9, 31 -- To: {microscopy-at-microscopy.com} 9, 31 -- X-OriginalArrivalTime: 20 Dec 2006 10:10:50.0183 (UTC) FILETIME=[1FF11D70:01C7241F] 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kBKAATOk006973 ==============================End of - Headers==============================
Anyone know of someone capable of repairing an Ultratome V in the Southeastern US? Ours suddenly quit working, and I've exhausted the simple stuff. TIA Julian -- Julian P.S. Smith III Dept. of Biology Winthrop University 520 Cherry Rd. Rock Hill, SC 29733
Hi all, I want to set up a long-term in-vivo imaging experiment using Saivi715 microspheres (invitrogen). The fluorescence excitation and emission is 715/755 nm. Does anyone have experience with this or similar microparticles using confocal system? Thanks and greetings, Zsolt
************************************** Zsolt Lazar, PhD Molecular Cytology Core Facility Memorial Sloan-Kettering Cancer Center 415 East 68th Street, ZRC-1838 New York, NY 10021
==============================Original Headers============================== 3, 34 -- From lazarz-at-mskcc.org Wed Dec 20 13:10:20 2006 3, 34 -- Received: from extmail.mskcc.org (extmail.mskcc.org [140.163.0.99]) 3, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBKJAKxn010899 3, 34 -- for {microscopy-at-microscopy.com} ; Wed, 20 Dec 2006 13:10:20 -0600 3, 34 -- Received: from 140.163.141.235 by extmail.mskcc.org with ESMTP (ESMTP 3, 34 -- Relay); Wed, 20 Dec 2006 14:10:15 -0500 3, 34 -- X-Server-Uuid: 74E28B0A-64D9-4563-A4EB-AECFC40E1CE3 3, 34 -- Received: from smskpexmbx3.MSKCC.ROOT.MSKCC.ORG ([140.163.141.252]) by 3, 34 -- smskpexsmtp2.MSKCC.ROOT.MSKCC.ORG with Microsoft 3, 34 -- SMTPSVC(6.0.3790.1830); Wed, 20 Dec 2006 14:10:15 -0500 3, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 3, 34 -- Content-class: urn:content-classes:message 3, 34 -- MIME-Version: 1.0 3, 34 -- Subject: Saivi715 microspheres 3, 34 -- Date: Wed, 20 Dec 2006 14:10:14 -0500 3, 34 -- Message-ID: {E1675D0E71BA5844925CA7653D64193115AD90-at-SMSKPEXMBX3.MSKCC.ROOT.MSKCC.ORG} 3, 34 -- X-MS-Has-Attach: 3, 34 -- X-MS-TNEF-Correlator: 3, 34 -- Thread-Topic: Saivi715 microspheres 3, 34 -- Thread-Index: AcckanrIVwacOVBTSw2zEpQRpMafbg== 3, 34 -- From: LazarZ-at-mskcc.org 3, 34 -- To: microscopy-at-microscopy.com 3, 34 -- X-OriginalArrivalTime: 20 Dec 2006 19:10:15.0715 (UTC) 3, 34 -- FILETIME=[7B4D5F30:01C7246A] 3, 34 -- X-TMWD-Spam-Summary: TS=20061220191016; SEV=2.0.1; DFV=A2006122010; 3, 34 -- IFV=2.0.4,4.0-8; RPD=4.00.0004; ENG=IBF; 3, 34 -- RPDID=303030312E30413031303230332E34353839383933392E303033352D412D; 3, 34 -- CAT=NONE; CON=NONE 3, 34 -- X-MMS-Spam-Filter-ID: A2006122010_4.00.0004_4.0-8 3, 34 -- X-WSS-ID: 6997551D29O1381872-01-01 3, 34 -- Content-Type: text/plain; 3, 34 -- charset=iso-8859-2 3, 34 -- Content-Transfer-Encoding: 8bit 3, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kBKJAKxn010899 ==============================End of - Headers==============================
Hello, I am looking for the information of wedge polisher. I would appreciate If you could provide the information of T-tool or other wedge polisher. ( I already have the information on tripod).
Hiromi Konishi, Ph.D. University of Wisconsin-Madison
==============================Original Headers============================== 3, 30 -- From hikonishi-at-gmail.com Wed Dec 20 17:24:23 2006 3, 30 -- Received: from py-out-1112.google.com (py-out-1112.google.com [64.233.166.183]) 3, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBKNOM8u002335 3, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 20 Dec 2006 17:24:23 -0600 3, 30 -- Received: by py-out-1112.google.com with SMTP id b50so1359238pyh 3, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 20 Dec 2006 15:24:21 -0800 (PST) 3, 30 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 3, 30 -- s=beta; d=gmail.com; 3, 30 -- h=received:message-id:from:to:subject:date:mime-version:content-type:content-transfer-encoding:x-priority:x-msmail-priority:x-mailer:x-mimeole; 3, 30 -- b=fQiwynJBbLjEvx8NbYhgw0WATa5NZRVStAEOHR+Hal1IJU7j4ZVsVVnWOvpf31zWPJGcBNlCxm/Ez+XHL3zlqZ/20kt62Pismy+kXiwu3Llh35PxJp2G9t8rlnGSgmKhMKsiu/5ZdcPxnHRZI+95X7d55THWHKVSp8gLT7ZPBYk= 3, 30 -- Received: by 10.35.80.20 with SMTP id h20mr13861548pyl.1166657060945; 3, 30 -- Wed, 20 Dec 2006 15:24:20 -0800 (PST) 3, 30 -- Received: from HKONISHI ( [144.92.207.106]) 3, 30 -- by mx.google.com with ESMTP id f24sm12411396pyh.2006.12.20.15.24.20; 3, 30 -- Wed, 20 Dec 2006 15:24:20 -0800 (PST) 3, 30 -- Message-ID: {001301c7248d$fd25f3a0$6acf5c90-at-HKONISHI} 3, 30 -- From: "Hiromi Konishi" {hikonishi-at-gmail.com} 3, 30 -- To: {Microscopy-at-microscopy.com} 3, 30 -- Subject: wedge polisher 3, 30 -- Date: Wed, 20 Dec 2006 17:24:20 -0600 3, 30 -- MIME-Version: 1.0 3, 30 -- Content-Type: text/plain; 3, 30 -- format=flowed; 3, 30 -- charset="iso-2022-jp"; 3, 30 -- reply-type=original 3, 30 -- Content-Transfer-Encoding: 7bit 3, 30 -- X-Priority: 3 3, 30 -- X-MSMail-Priority: Normal 3, 30 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 3, 30 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
Many thanks to all who gave a advice about our tray field and shielding question. They confirmed our thought, and we are studying that question further to find a aceptable solution.
It's difficult to find "clean" rooms... and even more to convince non microcopists about the demands of our instruments !
Jacques
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Hi all, My Reichert Ultracut S is having a problem - the top fluorescent lights won't come on and the motor won't switch on - seems like a board in the controller unit must have gone out. Has anyone had the same experience and if so willing to give advice on fixing it?
Thanks in advance and Happy Holidays!
Beth
********************************************************************** Beth Richardson EM Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
Phone - (706) 542-1790 & FAX - (706) 542-1805
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
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==============================Original Headers============================== 11, 19 -- From beth-at-plantbio.uga.edu Thu Dec 21 12:29:14 2006 11, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBLITEXx019746 11, 19 -- for {microscopy-at-microscopy.com} ; Thu, 21 Dec 2006 12:29:14 -0600 11, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 11, 19 -- (authenticated user beth-at-plantbio.uga.edu) 11, 19 -- by dogwood.plantbio.uga.edu 11, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 11, 19 -- for microscopy-at-microscopy.com; 11, 19 -- Thu, 21 Dec 2006 13:29:10 -0500 11, 19 -- Mime-Version: 1.0 (Apple Message framework v752.2) 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- Message-Id: {49D8A591-4D3E-4CC6-8D5E-7D5639FF4074-at-plantbio.uga.edu} 11, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 11, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 11, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 11, 19 -- Subject: problem with an Ultracut S 11, 19 -- Date: Thu, 21 Dec 2006 13:29:10 -0500 11, 19 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
I have sent the schematics to your email address as listed below.
John Brealey
Might be the same, I'd have to see them. Any chance you could scan and email them?
Allen R. Sampson, Owner Advanced Research Systems 317 North 4th. Street Saint Charles, Illinois 60174 phone (630) 513-7093 fax (630) 513-7092 email mailto:ars-at-sem.com web www.sem.com
On Tuesday, December 19, 2006 5:24 PM, john.brealey-at-imvs.sa.gov.au [SMTP:john.brealey-at-imvs.sa.gov.au] wrote: } } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } I have the schematics for the S-520 SEM evacuating sequence. } Is that what you're after? } } John Brealey } Queen Elizabeth Hospital EM Unit } Adelaide, South Australia } } } } } } } Hi all. } } I am hoping that someone could provide schematics for a Hitachi S-530 } SEM, particularly for the vacuum controller. I'm helping a college } revive an old instrument and don't have the documents for it. } Presently stuck on the vacuum controller not completing a pumpdown sequence. } } Allen R. Sampson, Owner } Advanced Research Systems } 317 North 4th. Street } Saint Charles, Illinois 60174 } phone (630) 513-7093 fax (630) 513-7092 email mailto:ars-at-sem.com web } www.sem.com }
==============================Original Headers============================== 10, 33 -- From john.brealey-at-imvs.sa.gov.au Thu Dec 21 16:43:37 2006 10, 33 -- Received: from adl0741.systems.sa.gov.au (adl0741.systems.sa.gov.au [143.216.236.20]) 10, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBLMhakH004158 10, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 21 Dec 2006 16:43:37 -0600 10, 33 -- Received: from adl0741.systems.sa.gov.au (localhost [127.0.0.1]) 10, 33 -- by adl0741.systems.sa.gov.au OUTGOING (8.12.10/8.12.10) with ESMTP id kBLMhYni027848 10, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 22 Dec 2006 09:13:34 +1030 (CST)' 10, 33 -- Received: from ablett.imvs.sa.gov.au (ablett.imvs.sa.gov.au [10.20.98.41]) 10, 33 -- by adl0741.systems.sa.gov.au INCOMING (8.12.10/8.12.10) with ESMTP id kBLMhY7X027845 10, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 22 Dec 2006 09:13:34 +1030 (CST)' 10, 33 -- Received: from localhost (mesh.imvs.sa.gov.au [10.20.98.37]) 10, 33 -- by ablett.imvs.sa.gov.au (Postfix) with ESMTP id 203DC34BFA 10, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 22 Dec 2006 09:13:34 +1030 (CST) 10, 33 -- X-Virus-Scanned: by amavisd-new at imvs.sa.gov.au 10, 33 -- Received: from ablett.imvs.sa.gov.au ([10.20.98.41]) 10, 33 -- by localhost (.imvs.sa.gov.au [10.20.98.37]) (amavisd-new, port 10024) 10, 33 -- with LMTP id oBHrYgoDgyKE for {Microscopy-at-MSA.Microscopy.Com} ; 10, 33 -- Fri, 22 Dec 2006 09:13:26 +1030 (CST) 10, 33 -- Received: from iqe36042 (iqepc200.imvs.sa.gov.au [10.20.138.200]) 10, 33 -- by ablett.imvs.sa.gov.au (Postfix) with SMTP id 30E4E34B82 10, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 22 Dec 2006 09:13:26 +1030 (CST) 10, 33 -- From: "John Brealey" {john.brealey-at-imvs.sa.gov.au} 10, 33 -- To: "Listserver" {Microscopy-at-MSA.Microscopy.Com} 10, 33 -- Subject: Hitachi S-520 Schematics 10, 33 -- Date: Fri, 22 Dec 2006 09:13:24 +1030 10, 33 -- Message-ID: {000001c72551$6d1d3d80$c88a140a-at-iqe36042} 10, 33 -- MIME-Version: 1.0 10, 33 -- Content-Type: text/plain; 10, 33 -- charset="us-ascii" 10, 33 -- Content-Transfer-Encoding: 7bit 10, 33 -- X-Mailer: Microsoft Office Outlook 11 10, 33 -- Thread-Index: AcclUWAY1XiZQ4VBRMynH+qIOuItwA== 10, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1807 ==============================End of - Headers==============================
I just wanted to say to all that I bought this recommended book and it is absolutely wonderful.
Thank you very much. What a wonderful addition to my library.
dj
On Thu, 7 Dec 2006, richard.beanland-at-bookham.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This thread is following the route to a Nobel prize in Physics.. have a } read of Richard Feynman's "QED-the strange theory of light and matter", } which addresses exactly this point and is a summary of the work for } which he was awarded his gong. I haven't read it for a few years, but I } remember him starting with glass being 80% transmissive, and asking how } could individual photons 'know' whether to cross the glass or not to } give the right answer of 80%. Like most of his stuff it is very } readable and entertaining. And yes, the world is actually weirder than } you think it is.. } } Richard } } ________________________________________ } Richard Beanland } Materials Analysis } Bookham } Caswell } Towcester } Northants } NN12 8EQ } United Kingdom } Tel. +44 1327 356362 } Fax. +44 1327 356775 } http://www.bookham.com } ________________________________________ } } -----Original Message----- } X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] } Sent: 06 December 2006 20:27 } To: Richard Beanland } Subject: [Microscopy] optical physics:Challenge } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } Greetings, } These and other wonderful answers to the Snell law question } have all been wave-based. Is there an equivalent photon-based answer? } Presumably, if you fired one photon at a time at an oblique surface } you would get the most counts at the Snell angle; but, with single } photons, how are we to understand lines of coherent marchers or even } two feet on roller blades? Is this light being weird? } } Just wondering... } Tobias } } } } } This is a really fine answer. I would add one more analogy, since } } the question (I think) relates to the change in direction of the path } } of the light beam. In my classes, I refer to the image of a column } } of a marching band going from a concrete surface to a broken field. } } If they are exactly perpendicular to the boundary, they continue to } } go straight, although with some stumbles. If they enter at an angle, } } then those members who are on the "acute" side of the column slow } } down first. This "pulls the others along as well, bit by bit, and } } the overall direction of the march changes. } } } } Date sent: Wed, 6 Dec 2006 11:21:25 -0600 } } To: jbs-at-temple.edu } } X-from: bfoster-at-mme1.com } } Send reply to: bfoster-at-mme1.com } } Subject: [Microscopy] Re: viaWWW: optical physics } } } } } } } } } } } } } } } } } } } Hi, } } } } } } The answer is really quite simple: Snell's Law. The bending of } } } light as it crosses the boundary from one RI to another is governed } } } by the Laws of Refraction. } } } } } } The bending discussed in your question requires that several } } } conditions be met: First, that there be different RIs on each side } } } of the boundary. Second, that the light approach the boundary at } } } an angle. } } } } } } To understand what happens, it is first necessary to understand } } } that refractive index is actually a measure of the impact of } } } interaction between the electric field of light and the electric } } } field of matter. The greater the interaction, the more slowly } } } light will travel through that material and the higher the } } } refractive index. For instance: } } } RI = velocity of light in air/velocity of light in material } } } RI of air = 1, velocity of light = 300,000 km/s } } } RI of water is 1.33, velocity of light is 225,000 km/s } } } RI of immersion oil, glass, and many polymers is approximately } } } 1.5, velocity of light = 200,000 km/sec. } } } } } } As for refraction, the analogy which is often used is that of a } } } person roller skating (or in this day and age, roller blading) from } } } one surface to another. For example, from the concrete sidewalk } } } onto grass. The smooth surface of the concrete is analogous to a } } } material with low refractive index; the rougher surface of glass is } } } analogous to a material with higher RI. If the skater approaches } } } the sidewalk:grass boundary with both feet parallel, both feet will } } } slow down by the same amount and the skater will continue skating } } } in the same direction. However, if the skater approaches the } } } boundary at an angle, one foot will slow down while the other } } } remains at the original speed. That different in speed causes the } } } skater to pivot toward the material of higher RI. } } } } } } If you think of light in terms of a wave front rather than a } } } simple ray, the analogy transfers easily. If the wave approaches a } } } boundary at an angle, the edge of the front which hits the higher } } } RI first will slow down, causing the whole wave front to pivot } } } around that point. That's what happens, for example, when light } } } travels from air (RI = 1.00) into a glass coverslip (RI ~1.5) or a } } } droplet of water or a cell (RI ~1.33). You can use the optical } } } axis of the microscope as a reference. In these cases, the light } } } will bend in TOWARD the optical axis. } } } Conversely, when light emerges from a glass slide or a droplet of } } } water it will bend AWAY from the OA. Actually this is why we use } } } oil immersion: to cause those rays which would normally bend away } } } from the OA, causing a loss of both intensity and the critical } } } contribution to resolution and edge definition, to bend back toward } } } the OA, where they have an opportunity to be captured by the } } } objective and make a positive contribution to better imaging. } } } } } } All of this is explained in detail, with diagrams in Optimizing } } } Light Microscopy. If you are interested in a copy, please contact } } } Ken Piel here in the MME office for purchasing details (see below). } } } } } } Hope this was helpful. } } } } } } Best regards, } } } Barbara Foster } } } } } } Microscopy/Microscopy Education } } } 313 S Jupiter Rd, Suite 100 } } } Allen, TX 75002 } } } P: 972-954-8011 } } } W: www.MicroscopyEducation.com } } } } } } } } } MME is now scheduling customized, on-site courses through next } } } April. Call us today for details. } } } } } } P. S. } } } Need a good general reference or light microscopy text for the } } } Spring semester? Call us today to learn more about "Optimizing } } } LIght Microscopy". Copies still available through MME... even for } } } class-room lots ... and we give quantity discounts. Call Ken Piel } } } at (972)954-8011 or email him at kenpiel-at-mme1.com } } } } } } } } } } } } At 08:24 AM 12/6/2006, vthawfeek-at-yahoo.co.in wrote: } } } } } } } } } } } } } } } } } } } This Question/Comment was submitted to the Microscopy Listserver } } } } using the WWW based Form at } http://www.microscopy.com/MLFormMail.html } } } } } ----------------------------------------------------------------------- } ---- } } } } Remember this posting is most likely not from a Subscriber, so } } } when replying } } } } please copy both vthawfeek-at-yahoo.co.in as well as the } } } MIcroscopy Listserver } } } } } ----------------------------------------------------------------------- } ---- } } } } } } } } Email: vthawfeek-at-yahoo.co.in } } } } Name: V.Thawfeek Mohammed } } } } } } } } Organization: psg tech coll. of engg. } } } } } } } } Title-Subject: [Filtered] optical physics } } } } } } } } Question: why does a light beam diverge when it strikes a medium } } } with greater refractive index than the medium ,in which it is. } } } } } } } } } ----------------------------------------------------------------------- } ---- } } } } } } -- } _ ____ __ ____ } / \ / / \ / \ \ Tobias I. Baskin } / / / / \ \ \ Biology Department } /_ / __ /__ \ \ \__ 611 N. Pleasant St. } / / / \ \ \ University of } Massachusetts } / / / \ \ \ Amherst, MA, 01003 } / / ___ / \ \__/ \ ____ } http://www.bio.umass.edu/biology/baskin/ } Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243 } } ==============================Original } Headers============================== } 6, 19 -- From baskin-at-bio.umass.edu Wed Dec 6 14:25:31 2006 } 6, 19 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu } [128.119.55.19]) } 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id kB6KPVfD011840 } 6, 19 -- for {microscopy-at-microscopy.com} ; Wed, 6 Dec 2006 } 14:25:31 -0600 } 6, 19 -- Received: from [172.30.55.79] (eutopia [128.119.55.30]) } 6, 19 -- by marlin.bio.umass.edu (8.13.6/8.13.6) with ESMTP id } kB6KPQZB025363; } 6, 19 -- Wed, 6 Dec 2006 15:25:27 -0500 (EST) } 6, 19 -- Mime-Version: 1.0 } 6, 19 -- Message-Id: {p0623090dc19cd66dc686-at-[172.30.55.79]} } 6, 19 -- In-Reply-To: {200612062000.kB6K0oST001676-at-ns.microscopy.com} } 6, 19 -- References: {200612062000.kB6K0oST001676-at-ns.microscopy.com} } 6, 19 -- Date: Wed, 6 Dec 2006 15:25:24 -0500 } 6, 19 -- To: microscopy-at-microscopy.com } 6, 19 -- From: Tobias Baskin {baskin-at-bio.umass.edu} } 6, 19 -- Subject: optical physics:Challenge } 6, 19 -- Cc: jbs-at-temple.edu } 6, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } 6, 19 -- X-Greylist: Sender succeded SMTP AUTH authentication, not } delayed by milter-greylist-1.6 (marlin.bio.umass.edu [128.119.55.19]); } Wed, 06 Dec 2006 15:25:29 -0500 (EST) } 6, 19 -- X-Scanned-By: MIMEDefang 2.54 on 128.119.55.19 } ==============================End of - } Headers============================== } } ======================================================================= } This e-mail is intended for the person it is addressed to only. 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==============================Original Headers============================== 6, 19 -- From dljones-at-bestweb.net Fri Dec 22 09:43:57 2006 6, 19 -- Received: from vms042pub.verizon.net (vms042pub.verizon.net [206.46.252.42]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBMFhvFu006278 6, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 22 Dec 2006 09:43:57 -0600 6, 19 -- Received: from localhost ([71.249.4.219]) 6, 19 -- by vms042.mailsrvcs.net (Sun Java System Messaging Server 6.2-6.01 (built Apr 6, 19 -- 3 2006)) with ESMTPA id {0JAO00480MCXOUP7-at-vms042.mailsrvcs.net} for 6, 19 -- Microscopy-at-microscopy.com; Fri, 22 Dec 2006 09:43:50 -0600 (CST) 6, 19 -- Date: Fri, 22 Dec 2006 10:43:45 -0500 (Eastern Standard Time) 6, 19 -- From: "David L. Jones" {dljones-at-bestweb.net} 6, 19 -- Subject: Re: [Microscopy] RE: optical physics:Challenge 6, 19 -- In-reply-to: {200612070947.kB79lwSs006322-at-ns.microscopy.com} 6, 19 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 6, 19 -- To: richard.beanland-at-bookham.com 6, 19 -- Cc: Microscopy-at-microscopy.com 6, 19 -- Message-id: {Pine.WNT.4.64.0612221037410.3156-at-H-F1} 6, 19 -- MIME-version: 1.0 6, 19 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 6, 19 -- References: {200612070947.kB79lwSs006322-at-ns.microscopy.com} ==============================End of - Headers==============================
For those who would like to get this from the horses mouth, as it were, I discovered a link that Feynman gave at the University of Auckland in 1979. This one is about properties of light, and accounts for bending of light and lenses (see about 52 minutes into the lecture) in a way that attempts to resolve the particle/wave issue. http://www.vega.org.uk/video/programme/46
Joel
Date sent: Fri, 22 Dec 2006 09:44:08 -0600 To: jbs-at-temple.edu X-from: dljones-at-bestweb.net Send reply to: dljones-at-bestweb.net
This Question was submitted to Ask-A-Microscopist by (tauria-at-hotmail.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, December 27, 2006 at 05:19:51 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both tauria-at-hotmail.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: tauria-at-hotmail.com Name: DR. FRANCIS J. PRONESTI
Organization: WORLD ENERGY SERVICES, LTD.
Education: Graduate College
Location: CASTELLANA GROTTE, BARI, ITALY
Title: ZEISS TEM 805 DOCUMENTATION
Question: HELLO, I NEED DESPERATELY TO BUY, BORROW OR STEAL A COPY OF SETPUP, OPERATION AND MAINTENANCE FOR A CZRL ZEISS TEM MICROSCOPE, MODEL 805.
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Email: mark.grimson-at-ttu.edu Name: Mark Grimson
Organization: Texas Tech University
Title-Subject: [Filtered] 3-D reconstruction from serial paraffin sections
Question: I am attempting to follow the path of cotton fibers around a seed in an attempt to see how they pack as they develop. I have embeedded the samples in paraffin and serially sectioned through the bundle in 12 micron sections. As such, I have almost 900 sections. Is there a program or method that can generate a 3-D reconstruction from some or all of these 2-D sections via light microscopy with a digital camera? Thank you. Mark
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Question: I am looking for the information of wedge polisher. I would appreciate it if you could provide the information of T-tool or other wedge polisher. ( I already have the information on tripod).
Thank you, Hiromi Konishi, Ph.D. University of Wisconsin-Madison
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Email: pedromfjcosta-at-googlemail.com Name: Pedro MFJ Costa
Organization: National Institute of Materials Science
Title-Subject: [Filtered] TEM current density measurements
Question: Dear Listers,
As part of a series of studies I am doing on beam damage of semiconductor structures, I have recently taken some measurements of the electron current in our JEOL 300 kV microscope. For this I relied on a retractable Faraday cup (located in the viewing chamber). My approach was as follows: I decided to investigate two modes (Low Mag and Mag1). This choice is justified by the fact that these are the ones we use for the IV experiments. For each mode I chose some representative magnifications to see if the effect of changing Mag would make any difference (I was not expecting any, but I thought it would be advisable to check). Within each Magnification I took readings using the Faraday cup (current values were read from a picoammeter). Since the Faraday cup was out-centred relative to the optical axis of the microscope, I had to shift the beam in between readings so that it would be centred with the Faraday cup's entrance axis (the need for centring comes from the fact that the intensity distribution is not uniform on the illuminated area but rather likely to follow a Gaussian-like curve). Taking into consideration that the entrance hole in the Faraday cup has a diameter of 1 mm, this means that the collection area will be: A = î?.r2 = 0.007854 cm2. So, if we have, for instance, 10000 pA read from the picoammeter for a Mag = 300 in an area of 0.007854 cm2 this would directly correspond to 1.273 ??A/cm2. In my experiments I used different measures of beam spread (fully converged beam, spreaded beam ?¯ limited by the negative makrers in the vieweing chamber fluorescent screen, almost parallel beam ?¯ approximately the size of the chamber viewing screen. So, from the above, using the aforementioned conditions, we get a maximum current density of 8.913 ??A/cm2 and a minimum of 0.382 nA/cm2. If finally we consider the relation for current density, j = eNv, where j is current density (A/cm2), e is the charge of an electron (C), N is the number of electrons per unit volume (particles/cm3) and v is the velocity of the electron, then we can estimate the electron flux density (i.e. the number of electrons per unit area per unit time) and this will give us, Minimum = 2.38E109 electrons/cm2/s, Maximum = 5.56E1013 electrons/cm2/s. Now my problem is whether my reasoning is correct. This is because I have seen many studies in the literature where current density values can be much higher (order of A/cm2). True that I am using a condenser aperture and this limits the number of electrons that we get from the gun. Also I expect that the settings of the guns electrostatic lens would make a difference but this, I suppose, is not accessible to the common user to change it. So, basically, it is mostly a matter of how much the beam is converged when it hits the sample. In my case no sample was used so there are no problems as to the electrons being backscattered/Rutherford scattered/etc by the specimen. For instance, I would expect that most of the electrons that come out from the condenser aperture would ultimately be collected in the reading devices (focus screen and Faraday cup) particularly for a converged beam (naturally there may be a percentage of electrons lost on their way down the column as they backscatter through the different column components but I am assuming that these are not significant). I understand that the density of collected electrons is not going to be similar in the Faraday cup, focus screen and fluorescent screen, as all these are in different imaging planes. Ideally we would like to have a holder that seats at the same height as the sample with a Faraday cup in it and take our readings at the same conditions as the imaging experimental settings. I think nonetheless that it may still be possible to use these readings and at least show a fairly reliable interval of current densities for our measures. So my questions are: Are my calculations correct in terms procedure? How come the values I get are so different from other quoted figures?
My best wishes,
Pedro -- Pedro MFJ Costa National Institute of Materials Science 1-1 Namiki, Tsukuba Ibaraki 305-0044 Japan
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (lazarz-at-mskcc.org) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, December 20, 2006 at 13:00:52 ---------------------------------------------------------------------------
Email: lazarz-at-mskcc.org Name: Zsolt Lazar
Organization: Memorial Sloan-Kettering Cancer Center
Education: Graduate College
Location: New York, NY
Question: Hi all, I want to set up a long-term in-vivo imaging experiment using Saivi715 microspheres (invitrogen). The fluorescence excitation and emission is 715/755 nm. Does anyone have experience with this or similar microparticles using confocal system? Thanks and greetings, Zsolt
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Organization: Comision Nacional de Energia Atomica- Argentina
Title-Subject: [Filtered] Problems with the EMS on line
Question: Dear Listers I used to run the free EMS on line (http://cimesg1.epfl.ch/CIOL) to draw SAD and Kikuchi patterns, (hkl) distances, etc., but I am having problems now. The legend "This calcul has failed for an undefined reason"always appeared when I try to run a routine. Can any body help me with it? Thanks in advanced! Patricia
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Email: onkel98-at-yahoo.com Name: pipetman
Title-Subject: [Filtered] Q: understanding STED?
Question: I have a problem conceptually understanding STED.
I can see, how a diffraction-limited spot of excited fluorophores is shaped to narrow the PSF. As a result, I get something akin to a point source of emission. But as the emitted light travels back through the microscope's optics, it is subject to diffraction again (and should thus destroy the narrowed PSF).
Is there somewhere a Gaussian fit applied at the other end (similar to PALM) to trace back the original spot? Or am I missing something basic here?
Any explanation would be greatly appreciated. Thanks - p
Check out 3D Constructor from Media Cybernetics: http://www.mediacy.com/index.aspx?page=3DConstructor
Best regards,
Walter F. Bobrowski Sr. Scientist Pfizer Global R&D 2800 Plymouth Rd. Ann Arbor, MI 48105
TEL: 734-622-7814 FAX: 734-622-5718
"The ultimate human freedom is the ability to choose one's attitude in a given set of circumstances." -Viktor Frankl
-----Original Message----- X-from: mark.grimson-at-ttu.edu [mailto:mark.grimson-at-ttu.edu] Sent: Wednesday, December 27, 2006 9:08 AM To: Bobrowski, Walter
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both mark.grimson-at-ttu.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: mark.grimson-at-ttu.edu Name: Mark Grimson
Organization: Texas Tech University
Title-Subject: [Filtered] 3-D reconstruction from serial paraffin sections
Question: I am attempting to follow the path of cotton fibers around a seed in an attempt to see how they pack as they develop. I have embeedded the samples in paraffin and serially sectioned through the bundle in 12 micron sections. As such, I have almost 900 sections. Is there a program or method that can generate a 3-D reconstruction from some or all of these 2-D sections via light microscopy with a digital camera? Thank you. Mark
==============================Original Headers============================== 6, 12 -- From zaluzec-at-microscopy.com Wed Dec 27 07:59:57 2006 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBRDxujB025496 6, 12 -- for {microscopy-at-microscopy.com} ; Wed, 27 Dec 2006 07:59:56 -0600 6, 12 -- Mime-Version: 1.0 6, 12 -- X-Sender: (Unverified) 6, 12 -- Message-Id: {p06110406c1b82ccd87e2-at-[206.69.208.22]} 6, 12 -- Date: Wed, 27 Dec 2006 07:59:55 -0600 6, 12 -- To: microscopy-at-microscopy.com 6, 12 -- From: mark.grimson-at-ttu.edu (by way of MicroscopyListserver) 6, 12 -- Subject: viaWWW: 3-D reconstruction from serial paraffin sections 6, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers============================== ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately.
==============================Original Headers============================== 19, 29 -- From Walter.Bobrowski-at-pfizer.com Wed Dec 27 20:33:02 2006 19, 29 -- Received: from mopmsgoa01.pfizer.com (mopmsgo.pfizer.com [148.168.100.84]) 19, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBS2X16u015093 19, 29 -- for {microscopy-at-microscopy.com} ; Wed, 27 Dec 2006 20:33:02 -0600 19, 29 -- Received: from groamrexc01.amer.pfizer.com (groamrexc01.amer.pfizer.com [172.30.8.168]) 19, 29 -- by mopmsgoa01.pfizer.com (8.13.4/8.13.4) with ESMTP id kBS2ViuL003247; 19, 29 -- Wed, 27 Dec 2006 21:31:44 -0500 19, 29 -- Received: from mopamrexc03.amer.pfizer.com ([170.116.30.69]) by groamrexc01.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.1830); 19, 29 -- Wed, 27 Dec 2006 21:33:04 -0500 19, 29 -- Received: from anaamrexm01.amer.pfizer.com ([162.48.101.10]) by mopamrexc03.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.1830); 19, 29 -- Wed, 27 Dec 2006 21:33:03 -0500 19, 29 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5.7226.0 19, 29 -- Content-class: urn:content-classes:message 19, 29 -- MIME-Version: 1.0 19, 29 -- Content-Type: text/plain; 19, 29 -- charset="US-ASCII" 19, 29 -- Subject: RE: [Microscopy] viaWWW: 3-D reconstruction from serial paraffin sections 19, 29 -- Date: Wed, 27 Dec 2006 21:33:02 -0500 19, 29 -- Message-ID: {CF89AF6ABD93B746A55BCCD9480A4B3D0844FE99-at-anaamrexm01.amer.pfizer.com} 19, 29 -- X-MS-Has-Attach: 19, 29 -- X-MS-TNEF-Correlator: 19, 29 -- Thread-Topic: [Microscopy] viaWWW: 3-D reconstruction from serial paraffin sections 19, 29 -- Thread-Index: AccpwHkH8NeqHHlRRSyqJkKUBwET+QAZ2pfQ 19, 29 -- From: "Bobrowski, Walter" {Walter.Bobrowski-at-pfizer.com} 19, 29 -- To: {mark.grimson-at-ttu.edu} , {microscopy-at-microscopy.com} 19, 29 -- X-OriginalArrivalTime: 28 Dec 2006 02:33:03.0703 (UTC) FILETIME=[7FF2DE70:01C72A28] 19, 29 -- X-Proofpoint-Spam-Reason: safe 19, 29 -- Content-Transfer-Encoding: 8bit 19, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id kBS2X16u015093 ==============================End of - Headers==============================
You might look at Reconstruct, http://synapses.bu.edu/tools/index.htm
It focused on reconstruction of serial TEM images but would probably work well for this application.
- Davi
Davi Bock Ph.D. student, Program in Neuroscience Harvard University
On Wed, Dec 27, 2006 at 08:07:03AM -0600, mark.grimson-at-ttu.edu wrote: } } Email: mark.grimson-at-ttu.edu } Name: Mark Grimson } } Organization: Texas Tech University } } Title-Subject: [Filtered] 3-D reconstruction from serial paraffin sections } } Question: I am attempting to follow the path of cotton fibers around a seed in an attempt to see how they pack as they develop. I have embeedded the samples in paraffin and serially sectioned through the bundle in 12 micron sections. As such, I have almost 900 sections. Is there a program or method that can generate a 3-D reconstruction from some or all of these 2-D sections via light microscopy with a digital camera? Thank you. Mark }
==============================Original Headers============================== 6, 17 -- From dbock-at-bostoncoop.net Thu Dec 28 11:16:50 2006 6, 17 -- Received: from bostoncoop.net (outbound.bostoncoop.net [72.1.169.236]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id kBSHGobJ009093 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 28 Dec 2006 11:16:50 -0600 6, 17 -- Received: by bostoncoop.net (Postfix, from userid 1011) 6, 17 -- id 6BA12A0146; Thu, 28 Dec 2006 12:16:49 -0500 (EST) 6, 17 -- Date: Thu, 28 Dec 2006 12:16:49 -0500 6, 17 -- From: Davi Bock {dbock-at-hms.harvard.edu} 6, 17 -- To: microscopy-at-microscopy.com 6, 17 -- Subject: Re: [Microscopy] viaWWW: 3-D reconstruction from serial paraffin sections 6, 17 -- Message-ID: {20061228171649.GA20306-at-bostoncoop.net} 6, 17 -- References: {200612271407.kBRE73GO023123-at-ns.microscopy.com} 6, 17 -- Mime-Version: 1.0 6, 17 -- Content-Type: text/plain; charset=us-ascii 6, 17 -- Content-Disposition: inline 6, 17 -- In-Reply-To: {200612271407.kBRE73GO023123-at-ns.microscopy.com} 6, 17 -- User-Agent: Mutt/1.5.9i ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (wawennekes-at-woh.rr.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, December 29, 2006 at 20:19:28 ---------------------------------------------------------------------------
Email: wawennekes-at-woh.rr.com Name: Willem Wennekes
Organization: UES
Education: Graduate College
Location: Dayton, Ohio, USA
Question: What is the difference in performance between a solid state & a scintilater (Robinson) backscatter detector? I am very interested in a side by side comparison.