Welcome to the 14th year of the Microscopy Listserver operation.
The complete searchable Microscopy Listserver archive for 2006 (~11.8 Mb) is now on-line at:
http://www.microscopy.com
Last year we delivered nearly 4.8 Million messages to subscribers around the world (~198.4 Gbits of traffic). I hate to say it but as in preceeding years spam attacks continue to increase. In 2006, the custom filters on the Listserver blocked 127,504 suspect postings for an average of 349.3 suspect spam messages/day. Just imagine what that would have done to your in-boxes as well as to the utility of this server.
As you probably realize, a infinitesimal number of those suspect Emails were actually from subscribers. The major problem continues to be hidden attachments.
Please if your message is rejected, read the entire rejection message for the reason and remember check the settings on your Email client program to insure that it is set to send only plain text messages. Most Email clients send formatted text as a hidden attachment to your mail, and this will be stopped by the Email filters. As always, there are no exceptions allowed. If you can't change the settings use the WWW based posting form at http://www.microscopy.com, this will strip any attachments from your message.
Cheers,
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 9, 11 -- From zaluzec-at-microscopy.com Mon Jan 1 10:48:51 2007 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l01GmpmK003674 9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 1 Jan 2007 10:48:51 -0600 9, 11 -- Mime-Version: 1.0 9, 11 -- Message-Id: {p06110400c1bee40981cb-at-[206.69.208.22]} 9, 11 -- Date: Mon, 1 Jan 2007 10:48:50 -0600 9, 11 -- To: microscopy-at-microscopy.com 9, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 9, 11 -- Subject: Adminsitrivia: 2006 Listserver Archives on-line 9, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Here is the January 2007 Microscopy Today table of contents. I will close the subscription list for this issue on Monday, Jan. 8th, 2007.
Microscopists in North America and MSA members anywhere can subscribe for free. Anyone else may subscribe for US$50 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com Thank you.
Ron Anderson, Editor ================================
Why Penguin Beaks are Sexy! Stephen W. Carmichael, ,Mayo Clinic
A ‘Different’ Kind of Microscopy Fred Schamber and Kai van Beek, Aspex Corporation
Microwave Myths and Tissue Processing Phillip McArdle, Energy Beam Sciences, East Granby, CT
Recent Developments in CrossBeam® Technology A. Thesen, H. Hoffmeister, M. Schumann, P. Gnauck, Carl Zeiss SMT Oberkochen, Germany
Heated-Tip AFM: Applications in Nanocomposite Polymer Membranes and Energetic Materials Jason P. Killgore1, William King2, Kevin Kjoller3 and René M. Overney1, 1 U. of Washington, Seattle, WA, 2 U. of Illinois at Urbana-Champaign, IL, 3 Anasys Instruments, Santa Barbara, CA
Automated S/TEM Sample Preparation for Semiconductor Process Support Greg Cuti* and Taha Jabbar**, *Sela USA, Inc. Sunnyvale, CA, and **Athenian Institute, Danville, CA
Serial Sectioning via Microtomy (or, How To Get Over 100 Consecutive Serial Sections On One TEM Gird) David Elliott, University of Arizona, Tucson, AZ
A Combined In-situ and Electron Tomography Holder for (S)TEM C. Mitterbauer*, N.D. Browning*,** and P. V. Deshmukh***,*U. of California, Davis, CA, **Lawrence Livermore National Lab., CA, ***E.A. Fischione Instruments, Inc., Export, PA
Infrared Laser Confocal Microscopy: Fast, Flexible, Cost-Effective Inspection, and Metrology Tool for Microelectronic Manufacturing David Rideout, Olympus Micro-Imaging Orangeburg, NY
New Approaches to Managing, Marketing, and Money for Maintaining a Core Facility (D. Sherman, Organizer) Part Ia: Case Study: Strategic plan for an EM Facility Elaine Humphrey
Part Ib: How to Make a Business Plan for Facility Maintenance and Growth Donald A. Blewett, Purdue University
A Note on Storing and Testing Gold Conjugates Jan Leunissen, Aurion, Costerweg, The Netherlands
Cross-sectional TEM Sample Preparation for Nanowires or Porous Films Grown on a Substrate Chengyu Song, NCEM, Lawrence Berkeley National Laboratory
New & Interesting at Cell Biology and Industry News
Netnotes SAMPLE PREPARATION – buffers for fixation SAMPLE PREPARATION – high pH buffer for fixation SAMPLE PREPARATION – cells grown on collagen gels SAMPLE PREPARATION - preservation of microbes in external mucous SAMPLE PREPARATION – charcoal and wood SAMPLE PREPARATION - embedding wood SAMPLE PREPARATION - propylene oxide vs. acetone SAMPLE PREPARATION - LR White flat embedding SAMPLE PREPARATION - LR White contrast SAMPLE PREPARATION – previously frozen tissues SAMPLE PREPARATION - embedding acrylamide gel SAMPLE PREPARATION – oil-in-water nanoemulsion SAMPLE PREPARATION - cross section of multilayers on stainless substrate SAMPLE PREPARATION – negative staining with ammonium molybdate LM - dark field microscopy LM - phase contrast vs. dark field TEM – digital cameras Crystallographic indices EBSD - sharpness EBIC vs. SE
Index of Advertisers
==============================Original Headers============================== 21, 18 -- From microscopytoday-at-tampabay.rr.com Mon Jan 1 11:32:58 2007 21, 18 -- Received: from ms-smtp-01.tampabay.rr.com (ms-smtp-01.tampabay.rr.com [65.32.5.131]) 21, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l01HWvZp014982 21, 18 -- for {Microscopy-at-Microscopy.Com} ; Mon, 1 Jan 2007 11:32:57 -0600 21, 18 -- Received: from [127.0.0.1] (rrcs-24-73-73-214.se.biz.rr.com [24.73.73.214]) 21, 18 -- by ms-smtp-01.tampabay.rr.com (8.13.6/8.13.6) with ESMTP id l01HWrsE002406; 21, 18 -- Mon, 1 Jan 2007 12:32:55 -0500 (EST) 21, 18 -- Message-ID: {459945C2.1020703-at-tampabay.rr.com} 21, 18 -- Date: Mon, 01 Jan 2007 12:32:50 -0500 21, 18 -- From: Microscopy Today {microscopytoday-at-tampabay.rr.com} 21, 18 -- User-Agent: Thunderbird 1.5.0.9 (Windows/20061207) 21, 18 -- MIME-Version: 1.0 21, 18 -- To: Listserver {Microscopy-at-Microscopy.Com} , 21, 18 -- Confocal Listserver {confocal-at-listserv.buffalo.edu} 21, 18 -- Subject: Microscopy Today January 2007 Table of Contents 21, 18 -- Content-Type: text/plain; charset=windows-1252; format=flowed 21, 18 -- Content-Transfer-Encoding: 8bit 21, 18 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both carnahan-at-edison-labs.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: carnahan-at-edison-labs.com Name: Jim Carnahan
Organization: Edison Analytical Laboratories, Inc.
Question: The computer, including the 8" floppy drives, on our ancient Tracor-Northern (Noran) TN-5500 detector died some time ago but now we would like to rejuvenate the instrument. I recall some years ago a company was selling a PC upgrade that used the existing HV, pulse processor and other boards but with a modern PC interface and software. Does anyone know if that product still exists and know the source?
An alternative for us would be if anyone has written a Labview (National Instruments) driver for these old instruments that we could implement.
Jim, One company that several of my customers have been happy with is IXRF http://www.ixrfsystems.com/ You can include a digital imaging option with it. No financial interest just good experience with them.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: carnahan-at-edison-labs.com [mailto:carnahan-at-edison-labs.com] Sent: Tuesday, January 02, 2007 9:32 AM To: kenconverse-at-qualityimages.biz
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying
please copy both carnahan-at-edison-labs.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: carnahan-at-edison-labs.com Name: Jim Carnahan
Organization: Edison Analytical Laboratories, Inc.
Question: The computer, including the 8" floppy drives, on our ancient Tracor-Northern (Noran) TN-5500 detector died some time ago but now we would like to rejuvenate the instrument. I recall some years ago a company was selling a PC upgrade that used the existing HV, pulse processor and other boards but with a modern PC interface and software. Does anyone know if that product still exists and know the source?
An alternative for us would be if anyone has written a Labview (National Instruments) driver for these old instruments that we could implement.
Original problem: File type omitted when saving images(E.G. picture instead of picture.tif). Also some menu windows had all the graphics, but omitted the text describing the "radio buttons" function. No other programs were malfunctioning. A photoshop reinstall did not fix the issue.
The problem was finally traced to something odd in the Windows 2000 user file. Have not figured out what or why, however. I installed an older hard drive clone, updated the windows install and now PS works fine.
Plan to clone the now functional (was backup clone) drive onto the problematic one. Looks like I will never find out what exactly happened - but it works so...
Thanks for all the suggestions,
Woody White BWXT Services
==============================Original Headers============================== 8, 26 -- From nwwhite-at-bwxt.com Tue Jan 2 12:19:19 2007 8, 26 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l02IJJgY013057 8, 26 -- for {microscopy-at-msa.microscopy.com} ; Tue, 2 Jan 2007 12:19:19 -0600 8, 26 -- Received: from ([131.184.13.224]) 8, 26 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.3446807; 8, 26 -- Tue, 02 Jan 2007 13:18:50 -0500 8, 26 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); 8, 26 -- Tue, 2 Jan 2007 13:18:50 -0500 8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 26 -- Content-class: urn:content-classes:message 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-Type: text/plain; 8, 26 -- charset="us-ascii" 8, 26 -- Subject: Photoshop glitch - Followup 8, 26 -- Date: Tue, 2 Jan 2007 13:18:49 -0500 8, 26 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB703D0FE-at-BWXSPO01.BWXS.BWXTECH.NET} 8, 26 -- X-MS-Has-Attach: 8, 26 -- X-MS-TNEF-Correlator: 8, 26 -- Thread-Topic: Photoshop glitch - Followup 8, 26 -- Thread-Index: AccumnNqN1dvz9CRRFOy9vWqnW5ZCg== 8, 26 -- From: "White, Woody N." {NWWhite-at-bwxt.com} 8, 26 -- To: "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 8, 26 -- X-OriginalArrivalTime: 02 Jan 2007 18:18:50.0495 (UTC) FILETIME=[73BCE8F0:01C72E9A] 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l02IJJgY013057 ==============================End of - Headers==============================
Hi listers! I used to run the free EMS on line (http://cimesg1.epfl.ch/CIOL) to draw SAD and Kikuchi patterns, (hkl) distances, indexing SAD patterns, etc., but I am having problems now. The legend "This calcul has failed for an undefined reason" always appeared when I try to run a routine. Can anybody help me with it? Thanks in advanced! Patricia
--------------------------------------------- Dra. Patricia B.Bozzano Lab. de Microscopia Electrónica Centro Atómico Constituyentes Comisión Nacional de Energía Atómica Buenos Aires, Argentina pbozzano-at-cnea.gov.ar Tel: 54 11 6772 7395 Fax: 54 11 6772 7362
==============================Original Headers============================== 7, 26 -- From pbozzano-at-cnea.gov.ar Tue Jan 2 12:26:16 2007 7, 26 -- Received: from cnea.gov.ar (cnea.gov.ar [168.96.65.229]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l02IQEb8019562 7, 26 -- for {Microscopy-at-Microscopy.Com} ; Tue, 2 Jan 2007 12:26:15 -0600 7, 26 -- Received: from cnea-mail.cnea.gov.ar (cnea-mail.cnea.gov.ar [168.96.68.244]) 7, 26 -- by cnea.gov.ar (8.11.2/8.11.2) with ESMTP id l02IRPh19720 7, 26 -- for {Microscopy-at-Microscopy.Com} ; Tue, 2 Jan 2007 15:27:25 -0300 7, 26 -- Received: from uam61 (uam61.cnea.gov.ar [168.96.65.126]) 7, 26 -- by cnea-mail.cnea.gov.ar (8.12.10/8.12.10) with ESMTP id l02IU3Fn008495 7, 26 -- for {Microscopy-at-Microscopy.Com} ; Tue, 2 Jan 2007 15:30:03 -0300 7, 26 -- From: "Patricia Bozzano" {pbozzano-at-cnea.gov.ar} 7, 26 -- To: {Microscopy-at-Microscopy.Com} 7, 26 -- Subject: EMS on line 7, 26 -- Date: Tue, 2 Jan 2007 15:26:46 -0300 7, 26 -- Message-ID: {000001c72e9b$8fd4e050$7e4160a8-at-uam61} 7, 26 -- MIME-Version: 1.0 7, 26 -- Content-Type: text/plain; 7, 26 -- charset="iso-8859-1" 7, 26 -- X-Priority: 3 (Normal) 7, 26 -- X-MSMail-Priority: Normal 7, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 7, 26 -- Importance: Normal 7, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 7, 26 -- X-RAVMilter-Version: 8.4.4(snapshot 20030410) (cnea-mail) 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l02IQEb8019562 ==============================End of - Headers==============================
} } Email: carnahan-at-edison-labs.com } Name: Jim Carnahan } } Organization: Edison Analytical Laboratories, Inc. } } Title-Subject: [Filtered] Tracor-Northern Xray detector. } } Question: The computer, including the 8" floppy drives, on our } ancient Tracor-Northern (Noran) TN-5500 detector died some time ago } but now we would like to rejuvenate the instrument. I recall some } years ago a company was selling a PC upgrade that used the existing } HV, pulse processor and other boards but with a modern PC interface } and software. Does anyone know if that product still exists and } know the source? } } An alternative for us would be if anyone has written a Labview } (National Instruments) driver for these old instruments that we } could implement. } } Thanks in advance, } } Jim Carnahan
Jim
One company that provides the service you mentioned is TN Analyzer, www.tnas.net. (run by a former Tracor-Northern engineer). We upgraded to their WinEDS system several years ago, at a very reasonable cost, and are happy with the product.
John -- ======================================================== John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480 Cameron Electron Microprobe Lab lab: (608) 265-4798 Dept of Geology & Geophysics fax: (608) 262-0693 University of Wisconsin home: (608) 274-2245 1215 West Dayton St. email: johnf-at-geology.wisc.edu Madison, WI 53706 amateur radio: WA3BTA Personal http://www.geology.wisc.edu/~johnf/ Probe lab http://www.geology.wisc.edu/~johnf/sx51.html Probe Sign Up Calender: http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi
"The first rule of all intelligent tinkering is to save every cog and wheel." -- Aldo Leopold
"For a successful technology, reality must take precedence over public relations, for Nature cannot be fooled." -- Richard P. Feynman
==============================Original Headers============================== 6, 26 -- From johnf-at-geology.wisc.edu Tue Jan 2 13:20:16 2007 6, 26 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l02JKGwm003181 6, 26 -- for {microscopy-at-microscopy.com} ; Tue, 2 Jan 2007 13:20:16 -0600 6, 26 -- Received: from localhost (localhost [127.0.0.1]) 6, 26 -- by localhost (Postfix) with ESMTP id 2D83320D1F; 6, 26 -- Tue, 2 Jan 2007 13:20:16 -0600 (CST) 6, 26 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) 6, 26 -- by localhost (geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 6, 26 -- with ESMTP id 09133-06; Tue, 2 Jan 2007 13:20:02 -0600 (CST) 6, 26 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57]) 6, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 6, 26 -- (No client certificate requested) 6, 26 -- by ice.geology.wisc.edu (Postfix) with ESMTP id 23EA020D05; 6, 26 -- Tue, 2 Jan 2007 13:20:02 -0600 (CST) 6, 26 -- Mime-Version: 1.0 6, 26 -- Message-Id: {p06230908c1c05f72f433-at-[144.92.206.57]} 6, 26 -- In-Reply-To: {200701021437.l02EbDLL027026-at-ns.microscopy.com} 6, 26 -- References: {200701021437.l02EbDLL027026-at-ns.microscopy.com} 6, 26 -- Date: Tue, 2 Jan 2007 13:16:25 -0600 6, 26 -- To: carnahan-at-edison-labs.com 6, 26 -- From: John Fournelle {johnf-at-geology.wisc.edu} 6, 26 -- Subject: Re: [Microscopy] viaWWW: Tracor-Northern Xray detector 6, 26 -- Cc: microscopy-at-microscopy.com 6, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 6, 26 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu ==============================End of - Headers==============================
We have both types of detector on one of our SEMs. While I can offer you some distinctions, others may have newer models and care to join the discussion.
Our SEM was originally supplied with a Robinson scintillator style detector. Its strengths were fast response for rapid scan rates up to and including TV-rate. It is good for general imaging and gives a nice topographic effect.
We later purchased a solid-sate detector for particular use for image analysis. Our application required lower voltage (6kV) than normal. We also required an even response across the field of view. For that, the solid-state detector was considerably better, but it has a slower response than the Robinson detector.
So the better detector will depend on your requirements. As it is, we run the solid state most of the time.
Warren Straszheim
________________________________________ X-from: wawennekes-at-woh.rr.com [mailto:wawennekes-at-woh.rr.com] Sent: Sat 12/30/2006 8:59 AM To: wesaia-at-iastate.edu
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (wawennekes-at-woh.rr.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, December 29, 2006 at 20:19:28 ---------------------------------------------------------------------------
Email: wawennekes-at-woh.rr.com Name: Willem Wennekes
Organization: UES
Education: Graduate College
Location: Dayton, Ohio, USA
Question: What is the difference in performance between a solid state & a scintilater (Robinson) backscatter detector? I am very interested in a side by side comparison.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sales-at-tradezone.net as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: At one time Evex (www.evex.com) of Princeton, NJ supplied x-ray microanalysis systems that interfaced to older systems like your Tracor 5500.
FYI: Though we currently don't have an inventory of tracor parts, you may want to check back with us in a few weeks to see if that changes.
Michael S. Used Laboratory Equipment Broker sales-at-TradeZone.net
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Martin.Hoppe-at-leica-microsystems.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Martin.Hoppe-at-leica-microsystems.com Name: Martin Hoppe
Organization: Leica Microsystems
Title-Subject: [Filtered] Re: Understanding STED
Question: In order to increase resolution with STED, it is not necessary to have a diffraction-unlimited detection point spread function (PSF). In STED, the diffraction-unlimited sharp excitation PSF is superimposed by the larger diffraction-limited detection PSF. In order to separate (=resolve) 2 adjacent points or molecules, they can both be located within the larger detection PSF. In STED, these points are sequentially excited due to the sharp excitation PSF in conjunction with scanning the beam across the specimen.
Regards Martin
-------------------------------------------------------- Martin Hoppe, Ph.D. Global Market Manager Advanced Fluorescence Systems Leica Microsystems CMS GmbH Am Friedensplatz 3 D 68165 Mannheim / Germany
Question: I have a problem conceptually understanding STED.
I can see, how a diffraction-limited spot of excited fluorophores is shaped to narrow the PSF. As a result, I get something akin to a point source of emission. But as the emitted light travels back through the microscope's optics, it is subject to diffraction again (and should thus destroy the narrowed PSF).
Is there somewhere a Gaussian fit applied at the other end (similar to PALM) to trace back the original spot? Or am I missing something basic here?
Any explanation would be greatly appreciated. Thanks - p
Recently I made an "appeal for spectra" to this listserve. To those who responded, we sincerely appreciate your contributions, and enthusiasm for the project. To those who did not respond because of my use of a personal email address, I have included my "FBI domain" address in the signature below. At the risk of redundancy I will repeat my original appeal (OK, Nestor?). Again, thank you.
} } } SEM/EDS has been important analytical instrumentation for the FBI for decades. We have recently expanded its utility in investigative roles, however, and therefore would like to ask the community for assistance. Whereas most SEM/EDS users need answers provided by quantitation and structural characterization, forensic inquiries generally necessitate "identifications"of questioned materials. This is generally possible only if the analyst is able to search his analytical results (spectra, primarily) against data from reference materials, as is the case with most other mature spectroscopies. We therefore have developed tools to archive and query the spectra and associated metadata of reference materials, and have produced a data set of thousands of materials in a variety of materials categories. Spectral and data queries against this database are consistently providing significant investigative direction. Of course quantitative analysis and other analytical techniques are additionally used where appropriate. Because the FBI has limited analytical capability and limited access to specific materials, I am appealing to you for either reference spectra that can be uploaded directly into our database, or materials that we can borrow and analyze at our facility (on a very limited, very specific basis). Literally all materials are viable candidates for inclusion, from commercial products to biologicals. Spectra must be exportable in EMSA format. Any data contributed will be restricted to FBI use only. If you would be willing to consider participating in this project, please contact me directly for details. We sincerely appreciate your consideration of this appeal!
Dennis Ward, FBI
Dennis Ward FBI Laboratory Chemistry Unit 2501 Investigation Parkway Quantico, VA 22135 v 703.632.7424
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mrsean-at-u.washington.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mrsean-at-u.washington.edu Name: Sean Kelly
Organization: University of Washington
Title-Subject: [Filtered] Can I process frozen tissue for TEM?
Question: I have some tissue that is embedded in OCT and stored at -70C and want to know if it can be processed for TEM.
Any experience with this or relevent publications would be greatly appreciated.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stuart-at-foto45.co.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: stuart-at-foto45.co.uk Name: Stuart Handley
Title-Subject: [Filtered] Photography
Question: Hi,the reason for ask a massive favour is, I am starting a masters degree in photography and related media in a few days time and need to put my proposal in for my research project. I am going to propose images and related images on the subject of mines and minerals. This will involve photography underground and of samples that I collect, my hope is that I can find an electron microscopists who can help me to get down to the micro level of the minerals. The photography has to be different or done in a way that has not been done before so by combining the conventional and the electro scanning microscope images plus studio images of minerals I will be able to achive my set goal. Can any one out there help in any way or put me on to some one who maybe able to. Finding access to such equipment is going to be hard, any help most appreciated, I'm doing the MA part time over three years. Stuart Handley
Hi Sean, Can you process it? Yes. Should you process it? Maybe. Will you get good ultrastrucure? No.
A lot will depend on how the tissue was treated before it was frozen: was it flash frozen without any fixation or cryo-protection? It will have a lot of freezing artefacts at the EM level...ice crystal damage to membranes, etc. If it was lightly fixed (eg:paraformaldehyde), it might fair a bit better. If it was fixed AND cryo-protected (eg: treated with sucrose), you might get some decent structure.
You'll have to cut away as much of the OCT as you can and then thaw the tissue. I would advise thawing it in a buffered glut. solution so that it fixes as it thaws. Then process for TEM as usual and keep you fingers crossed. Over the years, I've had to process tissues that were not originally intended for EM and I've been surprised by what may be rescued. Good luck, Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175 http://www.cornellcelldevbiology.org http://www.cornellbiochem.org
==============================Original Headers============================== 3, 24 -- From lcgould-at-med.cornell.edu Thu Jan 4 08:06:52 2007 3, 24 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l04E6qHt017830 3, 24 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jan 2007 08:06:52 -0600 3, 24 -- Received: from mpx1.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) 3, 24 -- by smtp-gw2.med.cornell.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l04E6dIe025222; 3, 24 -- Thu, 4 Jan 2007 09:06:50 -0500 (EST) 3, 24 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23]) 3, 24 -- by mpx1.med.cornell.edu 3, 24 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 3, 24 -- with ESMTPA id {0JBC009AJKJ2MF10-at-mpx1.med.cornell.edu} ; Thu, 3, 24 -- 04 Jan 2007 09:06:39 -0500 (EST) 3, 24 -- Date: Thu, 04 Jan 2007 09:06:36 -0500 3, 24 -- From: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu} 3, 24 -- Subject: Re: [Microscopy] Can I process frozen tissue for TEM? 3, 24 -- In-reply-to: {200701040120.l041K3Md005942-at-ns.microscopy.com} 3, 24 -- Sender: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu} 3, 24 -- To: Microscopy Listserver {microscopy-at-microscopy.com} , mrsean-at-u.washington.edu 3, 24 -- Message-id: {p0623092ac1c2b8f49172-at-[140.251.48.23]} 3, 24 -- MIME-version: 1.0 3, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed 3, 24 -- Content-transfer-encoding: 7BIT 3, 24 -- References: {200701040120.l041K3Md005942-at-ns.microscopy.com} 3, 24 -- X-PMX-Version: 5.2.2.285561, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.1.4.55433 ==============================End of - Headers==============================
If you are looking for art, I'd really suggest that you consider polarized light (an optical microscopy technique). The sample prep is about the same and the results are incredibly beautiful.
I have a colleague in the UK who can probably help. Contact me off-line for further info.
Good hunting! Barbara Foster
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
MME is now scheduling customized, on-site courses through April. Call us today for details.
P. S. Need a good general reference or light microscopy text for the Spring semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 11:26 AM 1/4/2007, stuart-at-foto45.co.uk wrote:
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There is a neat book: "Photography Through The Microscope" By J. G. Delly and Eastman Kodak. It has many examples of photos taken through light microscopes along with easy to follow 'how to...' instructions. My copy was published in 1988. I don't know if the book is still in print, but your University library can most likely has it or find a copy for you.
Take care, Bob Carter 2000 Bayshore Road Lopez Island, WA 98261-8595
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This Question was submitted to Ask-A-Microscopist by (steve.klingaman-at-normandale.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, January 4, 2007 at 14:07:06 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both steve.klingaman-at-normandale.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: steve.klingaman-at-normandale.edu Name: Steve Klingaman
Organization: Normandale Community College
Education: Undergraduate College
Location: Bloomington, MN USA
Title: SEM
Question: Can you make a recommendation as to an entry-level SEM for teaching purposes to be used in a life sciences lab? Would a "table top" model suffice?
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Question: Hi folks, I am trying to buy a used Reichert/Leica Cryo-ultramicrotome. I really only need the cryokit if you have one lying around or in storage. Both of our units have failed and we cant get the parts to fix them!!!! Built in obsolescence I guess. If you have a system, or know someone who would like to sell one to my group, please contact me offline Simon
Simon C. Watkins Ph.D. FRC Path. Professor, Cell Biology and Physiology and Immunology Director Graduate Program in Cell Biology and Physiology Vice Chair, Cell Biology and Physiology Director Center for Biologic Imaging BSTS 225 University of Pittsburgh 3500 Terrace St. Pittsburgh PA 15261 Tel:412-648-3051 Fax:412-648-2797 URL: http://www.cbi.pitt.edu
Question: Hi, Folks, I have a Philips CM20 traditional TEM with an EDS system. It is about 15 years old, but has a good working condition. Could you please tell me how much it is worth now? Thanks, Jian-Guo
------------------------------------------------------------------ Jian-Guo Zheng, PhD Director, Nanomaterials Characterization and Fabrication Facility (NCF2) University of California, Irvine 3415 Calit2 Building Irvine, CA 92697-2800 phone: 949-824-0441 (office), 949-468-9980 (cell) fax: 949-824-8197 email: jzheng-at-calit2.uci.edu ----------------------------------------------------------------
==============================Original Headers============================== 5, 20 -- From jzheng-at-uci.edu Thu Jan 4 19:39:19 2007 5, 20 -- Received: from relay2.es.uci.edu (relay2.es.uci.edu [128.200.80.28]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l051dIBg022095 5, 20 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jan 2007 19:39:19 -0600 5, 20 -- Received: from webmail.uci.edu (webmail2.es.uci.edu [128.200.80.37]) 5, 20 -- by relay2.es.uci.edu (8.13.1/8.13.1) with ESMTP id l051dHai009969 5, 20 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jan 2007 17:39:18 -0800 5, 20 -- Received: from 128.195.177.193 5, 20 -- (SquirrelMail authenticated user jzheng) 5, 20 -- by webmail.uci.edu with HTTP; 5, 20 -- Thu, 4 Jan 2007 17:39:18 -0800 (PST) 5, 20 -- Message-ID: {4616.128.195.177.193.1167961158.squirrel-at-webmail.uci.edu} 5, 20 -- Date: Thu, 4 Jan 2007 17:39:18 -0800 (PST) 5, 20 -- Subject: question: value of CM20 TEM 5, 20 -- From: jzheng-at-uci.edu 5, 20 -- To: microscopy-at-microscopy.com 5, 20 -- User-Agent: SquirrelMail/1.4.9a 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain;charset=iso-8859-1 5, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Back again with my basic questions about SEM ;-) I took pictures using both low vac mode (1 Pa) and high vac mode (8x10^-3 Pa) at magnifications from 3kX up to 25kX with my tescan SEM (at 10kV with quartz). I can't see the slightest improvement in image quality! The only difference is a slight increase in contrast at higher vacuum (which is not necessarily better because low-contrasted parts are not visible).
Is it normal? What would be the use of high vacuum? Is it useful for EDX analysis but not normal scanning?
Stephane
__________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com
==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Fri Jan 5 04:04:12 2007 6, 19 -- Received: from web37415.mail.mud.yahoo.com (web37415.mail.mud.yahoo.com [209.191.91.147]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l05A4BRJ008576 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jan 2007 04:04:12 -0600 6, 19 -- Received: (qmail 71687 invoked by uid 60001); 5 Jan 2007 10:04:11 -0000 6, 19 -- Message-ID: {20070105100411.71685.qmail-at-web37415.mail.mud.yahoo.com} 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 6, 19 -- b=6Gx+ph2PmuD2WzkOGIN+ZlNa+5yDUpTAndMMum4S8pYohGncsSeRk3Y7gMi6xMHk2L8saJZQYR9Ua9syw5S6OPvXMIxaLqqTfcgXnIml3pIjv4hRBM8f/yFB8L0wO40wgWkLiX5hO0T1umkDdEV/F9lswkOv0zraYu2BOPpL2kM=; 6, 19 -- X-YMail-OSG: Dytz84gVM1n9PsZNUfrMuvo5W9jrKdFdIN.p9A8I14W61PGS.hkHntDikHM.T.jW9sjQILdZH0BQYhaajWnaO7Gq3f6jKqQbihJLFOF4BgLBiSDijNJINair5E1.bkxQcrxj9g4_hWHDowA- 6, 19 -- Received: from [80.122.101.102] by web37415.mail.mud.yahoo.com via HTTP; Fri, 05 Jan 2007 02:04:11 PST 6, 19 -- Date: Fri, 5 Jan 2007 02:04:11 -0800 (PST) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 -- Subject: low vac SEM questions 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Conc: } Zeil Neelson {, } Zeil-Neilson {, } Zeil Neelsen {, } Zeil-Neilsen {, } Ziehl-Nielson {: which "name"/"term" one is correct
Dear all, I would like to ask a question which came into minds when ToC-reading the title of apreviousely published article on: } Zeil Neelson [sic!]and Wade-Fite stains to demonstrate medlar bodies of chromoblastomycosis {...
Can anybody of the group could tell or inform me wether a } Zeil-Neelson {(SIC!) stain (for TB and mycobacterium, acid fast bacilli) really does exist or is an other, classical, histochemical, specific stain - I always thought to correctly be called "Ziehl-Nielson"....
When googleing shortly for } Zeil-Neelson { I found out that there exist some other "terms" sounding very similar....see above....
Have I missed something??
Any information gladly is appreciated,
regards and THANK YOU,
Wolfgang Muss Salzburg-AUSTRIA
OR Dr. Wolfgang Muss Head of EM-Lab {=with pride: 25 years in operation by 2nd of Feb.2006=} Institute of Pathology, SALK (Salzburger Landeskliniken gemeinnuetzige GesmbH) (privatized General Hospital Fed. State of Salzburg) Muellner Hauptstrasse 48 A-5020 SALZBURG AUSTRIA/EUROPE
and/or/alternatively (same Lab, same address)
Paracelsus Medical Private University (PMU) Institute of Pathology Electron Microscopy Lab Muellner Hauptstrasse 48 A-5020 SALZBURG, Austria/Europe Phone work: +43+662+4482+4720 Mobile phone work:+43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss") E-Mail work: W.Muss-at-SALK.at
Ankuendigung namens der (Information on behalf of) Society for Cutaneous Ultrastructure Research (SCUR) PLEASE VISIT THE UPDATED WEBSITE of SCUR at } http://www.scur.org { ------------------------------------------------------------------------- post-congress information: 33rd Annual Meeting of the SCUR, 8th-10th June, 2006, WARSZAW, Poland For information visit the WEBSITE, http://www.scur.org/ Click: Meetings ==} Previous Meetings ==} 33rd. Ann. Meeting or, directly: http://orgs.dermis.net/content/e04scur/e03meetings/e775/e896/index_ger.html
Forthcoming Meetings: 34th Annual Meeting of the SCUR, June 14-16, 2007,PRAGUE Czech Republic Local Host: Prof. Dr. Peter ARENBERGER e-mail: pa-at-verum.cz
correct dates of meeting to be updated ASAP 35th Annual Meeting of the SCUR, ?11th -12th? May, 2008, OTHSU near KYOTO, Japan Joint Meeting with the JSUCB, the Japanese Society for Ultrastructural Cutaneous Biology
==============================Original Headers============================== 23, 40 -- From W.Muss-at-salk.at Fri Jan 5 04:13:00 2007 23, 40 -- Received: from hermes.salk.at (hermes.lks.at [193.170.167.9]) 23, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l05ACxrH017009 23, 40 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jan 2007 04:13:00 -0600 23, 40 -- Received: from localhost (localhost [127.0.0.1]) 23, 40 -- by hermes.salk.at (Postfix) with ESMTP id 5EE56C386F 23, 40 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jan 2007 11:12:58 +0100 (CET) 23, 40 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 23, 40 -- Received: from hermes.salk.at ([127.0.0.1]) 23, 40 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 23, 40 -- with ESMTP id 0BokYcsAqnZH for {microscopy-at-microscopy.com} ; 23, 40 -- Fri, 5 Jan 2007 11:12:58 +0100 (CET) 23, 40 -- Received: from hermes.lks.at (hermes.salk.at [192.168.13.210]) 23, 40 -- by hermes.salk.at (Postfix) with ESMTP id F39AAC3868 23, 40 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jan 2007 11:12:57 +0100 (CET) 23, 40 -- Received: from localhost (localhost [127.0.0.1]) 23, 40 -- by hermes.lks.at (Postfix) with ESMTP id DC0445A9022 23, 40 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jan 2007 11:12:57 +0100 (CET) 23, 40 -- Received: from hermes.lks.at ([127.0.0.1]) 23, 40 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024) 23, 40 -- with ESMTP id 13523-01 for {microscopy-at-microscopy.com} ; 23, 40 -- Fri, 5 Jan 2007 11:12:57 +0100 (CET) 23, 40 -- Received: from c1pa003 (unknown [192.168.42.3]) 23, 40 -- by hermes.lks.at (Postfix) with SMTP id 8F8075A901F 23, 40 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jan 2007 11:12:57 +0100 (CET) 23, 40 -- Received: by localhost with Microsoft MAPI; Fri, 5 Jan 2007 11:12:56 +0100 23, 40 -- Message-ID: {01C730BA.73B46140.W.Muss-at-salk.at} 23, 40 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at} 23, 40 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at} 23, 40 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 23, 40 -- Subject: [Microscopy] STAINING: Zeil Neelson, Zeil-Neilson,Zeil Neelsen, Zeil Neilsen, Ziehl-Nielson: which one is correct 23, 40 -- Date: Fri, 5 Jan 2007 11:12:55 +0100 23, 40 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at} 23, 40 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor 23, 40 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211 23, 40 -- MIME-Version: 1.0 23, 40 -- Content-Type: text/plain; charset="us-ascii" 23, 40 -- Content-Transfer-Encoding: 7bit 23, 40 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at 23, 40 -- X-Scanned-By: SALK-Content-Filter ==============================End of - Headers==============================
} Back again with my basic questions about SEM ;-) I took } pictures using both low vac mode (1 Pa) and high vac mode } (8x10^-3 Pa) at magnifications from 3kX up to 25kX with my } tescan SEM (at 10kV with quartz). I can't see the slightest } improvement in image quality! } The only difference is a slight increase in contrast at } higher vacuum (which is not necessarily better because } low-contrasted parts are not visible).
You do not give any indication of the beam current you are using (possibly the 'spot size' adjustment for your SEM). I suspect you will not realize the real advantages of employing high vacuum unless the beam scuurent (and spot size) is small. Better vacuum will also provide better contrast ... But exactly what you see is specimen and preparation dependent. I suspect the samples are coated ... But with ??
cheerios :o) michael shaffer
SEM/MLA Research Coordinator {http://www.mun.ca/creait/maf/} Inco Innovation Centre Memorial University St. John's, Newfoundland
==============================Original Headers============================== 6, 20 -- From michael-at-shaffer.net Fri Jan 5 07:06:48 2007 6, 20 -- Received: from n034.sc1.he.tucows.com (fh1021.dia.cp.net [64.97.168.31] (may be forged)) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l05D6mgZ001124 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jan 2007 07:06:48 -0600 6, 20 -- Received: from roamingwolf (134.153.130.141) by n034.sc1.he.tucows.com (7.2.069.1) (authenticated as Michael-at-Shaffer.net) 6, 20 -- id 458A9BAC0021124E; Fri, 5 Jan 2007 13:06:47 +0000 6, 20 -- From: "michael shaffer" {michael-at-shaffer.net} 6, 20 -- To: {nizets2-at-yahoo.com} 6, 20 -- Cc: "MSA Microscopy list" {Microscopy-at-microscopy.com} 6, 20 -- Subject: RE: [Microscopy] low vac SEM questions 6, 20 -- Date: Fri, 5 Jan 2007 09:36:34 -0330 6, 20 -- Message-ID: {001501c730ca$5b1b47b0$8d829986-at-roamingwolf} 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="US-ASCII" 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- X-Mailer: Microsoft Office Outlook 11 6, 20 -- In-Reply-To: {200701051004.l05A4oPC009282-at-ns.microscopy.com} 6, 20 -- Thread-Index: AccwsLAWLLyNoD6YR6+msTLKPCGAKgAGNgFA 6, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 ==============================End of - Headers==============================
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Question: Dear Listers: The Link exL hard drive crashed a month ago. We need an inexpensive temporarily solution. If anyone have a it somewhere in the lab, please let me know. It is a 40S 40MB Quantum Prodrive. I could purchase an HD online but I am not sure if it can be installed with Link software. Thank you.
In cleaning out the lab I came across a drawer full of small cylinders that have "Polaroid Print Coater" embossed on one side and "For Black & White Pictures Only". Inside is a fluid drenched material with a handy little holder along one side. I've never used them and we no longer have need to process film in the dark room but I believe the material is used to add a protective coating to the processed photographs. Is there anyone that would be interested in having some or all of these materials? In addition we have some powdered Kodak Developer D-19 and while I can't find an expiration date I'm sure it is several years old (I've been here over 5 years and it was purchased before my arrival.) There is also some EM film I'm sure has expired as well as some polaroid film and even a role of film called "Kodachrome 64". I also came across some liquid activator and fixer that was never opened. If you are interested in any or all of these items please contact me directly for more details.
Thanks! Angie
==============================Original Headers============================== 2, 23 -- From Anjeanette.Ormonde-at-unilever.com Fri Jan 5 07:58:28 2007 2, 23 -- Received: from ntrsegw30001.s3.ms.unilever.com (mailout02.unilever.com [204.110.170.4]) 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l05DwSSp023886 2, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jan 2007 07:58:28 -0600 2, 23 -- Received: from NTRSEVS30001.s3.ms.unilever.com ([162.87.40.60]) by ntrsegw30001.s3.ms.unilever.com with Microsoft SMTPSVC(6.0.3790.1830); 2, 23 -- Fri, 5 Jan 2007 08:58:28 -0500 2, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 23 -- Content-class: urn:content-classes:message 2, 23 -- MIME-Version: 1.0 2, 23 -- Content-Type: text/plain; 2, 23 -- charset="US-ASCII" 2, 23 -- Subject: Supplies for film processing to give away 2, 23 -- Date: Fri, 5 Jan 2007 08:58:26 -0500 2, 23 -- Message-ID: {DBE4410766F3FE4991E69A1CD3CA13AA01240A51-at-NTRSEVS30001.s3.ms.unilever.com} 2, 23 -- X-MS-Has-Attach: 2, 23 -- X-MS-TNEF-Correlator: 2, 23 -- Thread-Topic: Supplies for film processing to give away 2, 23 -- Thread-Index: AccwINGrFgMmmlrVRx28VMx/O/3OsAAsCWdQ 2, 23 -- From: "Ormonde, Anjeanette" {Anjeanette.Ormonde-at-unilever.com} 2, 23 -- To: {Microscopy-at-microscopy.com} 2, 23 -- X-OriginalArrivalTime: 05 Jan 2007 13:58:28.0302 (UTC) FILETIME=[936CAEE0:01C730D1] 2, 23 -- Content-Transfer-Encoding: 8bit 2, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l05DwSSp023886 ==============================End of - Headers==============================
First of all, my best wishes for 2007 - May the Light shine through your Microscope!
As often in this lab however, the beginning of a new year brings new challenges. We are looking for software to perform stereology analysis (Cavalieri, Fractionator, ...). At the moment we are using already one packge from Zeiss, incorporated into their KS300 software, however, we would like to expand the analysis to more pc's. Therefore the following question: is someone out there using the stereology modules for Image J? And if so, is there a manual available or could you pass some tips? Many thanks in advance,
One of the problems with assessing SEM performance is often the specimen you select. I travel around the world looking at customers instruments training the people to obtain more from their instrument. In order to judge the performance of the instruments I spent a number of months experimenting with test specimens until a came up with one where the image always changed in some way when I changed parameters.
I remember a ListServer posting some time ago when a scientist commented that the new FEG instruments are amazing as they are just as good (with their specimen) at 1kV as 30kV! In my mind the test specimen was not testing and I feel this could be the reason that you do not see changes when you vary the parameters in your instrument.
I poor quality vacuum is great to reduce charge. But a poor vacuum causes beam spread, a problem that will increase the spot size and, if sufficient performance is demanded, ultimately degrade the image quality.
Steve Chapman Senior Consultant Protrain For electron microscopy consultancy and training world wide Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967 Web www.emcourses.com
----- Original Message ----- X-from: {nizets2-at-yahoo.com} To: {protrain-at-emcourses.com} Sent: Friday, January 05, 2007 10:05 AM
Dear Stephane, There are several fundamental differences between high vac and low vac that make each unique in application. 1. low vac can only use BSE or special low vac SE-type detectors that make traditional, high-resolution SE images impossible. Low vac is usually 10 to 200 Pa. Usually these detectors require higher beam voltage (10 to 20 kV) and beam current than high-vac SE imaging. 2. High vac requires that a sample be conductive. Try your experiment with a freshly-plucked flower, looking at the stamen and pollen grains and you will see that it is impossible in high vac mode but quite possible in low vac. You may have to increase the gas pressure to 20 to 100 Pa to eliminate charging. If you need to do high resolution imaging of the small features on a sample surface, only high vac will allow you to use {5 kV, small aperture, short working distance and low beam current to get detailed SE images of the sample. 3. The scattering of the electron beam in low vac mode gives EDS contributions from areas away from where you may want to analyse. It is nice to be able to do EDS on anything without coating, but if you need to analyse one small spot without contribution from neighbouring areas, you need high vac. Both modes have their place and strengths and it is nice to have the choice. Regards, Mary Mager Electron Microscopist Materials Eng. UBC #419 - 6350 Stores Rd. Vancouver, B.C. V6T 1Z4 CANADA Phone: 604-822-5648 Fax: 604-822-3619 email: mager-at-interchange.ubc.ca
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Friday, January 05, 2007 2:14 AM To: mager-at-interchange.ubc.ca
Hi!
Back again with my basic questions about SEM ;-) I took pictures using both low vac mode (1 Pa) and high vac mode (8x10^-3 Pa) at magnifications from 3kX up to 25kX with my tescan SEM (at 10kV with quartz). I can't see the slightest improvement in image quality! The only difference is a slight increase in contrast at higher vacuum (which is not necessarily better because low-contrasted parts are not visible).
Is it normal? What would be the use of high vacuum? Is it useful for EDX analysis but not normal scanning?
Stephane
__________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com
==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Fri Jan 5 04:04:12 2007 6, 19 -- Received: from web37415.mail.mud.yahoo.com (web37415.mail.mud.yahoo.com [209.191.91.147]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l05A4BRJ008576 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jan 2007 04:04:12 -0600 6, 19 -- Received: (qmail 71687 invoked by uid 60001); 5 Jan 2007 10:04:11 -0000 6, 19 -- Message-ID: {20070105100411.71685.qmail-at-web37415.mail.mud.yahoo.com} 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Conten t-Transfer-Encoding:Message-ID; 6, 19 -- b=6Gx+ph2PmuD2WzkOGIN+ZlNa+5yDUpTAndMMum4S8pYohGncsSeRk3Y7gMi6xMHk2L8saJZQYR 9Ua9syw5S6OPvXMIxaLqqTfcgXnIml3pIjv4hRBM8f/yFB8L0wO40wgWkLiX5hO0T1umkDdEV/F9 lswkOv0zraYu2BOPpL2kM=; 6, 19 -- X-YMail-OSG: Dytz84gVM1n9PsZNUfrMuvo5W9jrKdFdIN.p9A8I14W61PGS.hkHntDikHM.T.jW9sjQILdZH0BQ YhaajWnaO7Gq3f6jKqQbihJLFOF4BgLBiSDijNJINair5E1.bkxQcrxj9g4_hWHDowA- 6, 19 -- Received: from [80.122.101.102] by web37415.mail.mud.yahoo.com via HTTP; Fri, 05 Jan 2007 02:04:11 PST 6, 19 -- Date: Fri, 5 Jan 2007 02:04:11 -0800 (PST) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 --
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Email: renaudgeological-at-execulink.com Name: Jim Renaud
Organization: Renaud Geological Consulting Ltd.
Title-Subject: [Filtered] WIN 30 PGH Board
Question: I am looking for a WIN 30 PGH board for the digital imaging system on my electron microprobe. I have checked with the manufacturer and this is a discontinued product. If you have one of these boards and would like to sell it or know here I may be able to find one, I would appreciate any information you could share.
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Email: eoptics-at-mcmaster.ca Name: Fred Pearson
Organization: McMaster Unviersity/Canadian Center for Electron Microscopy
Title-Subject: [Filtered] Conical Dark Field Imaging
Question: Any there any descriptive articles available for Conical Dark Field imaging and its advantages?
This Question was submitted to Ask-A-Microscopist by (Lori_mahaffey-at-yahoo.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, January 7, 2007 at 17:14:47 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both Lori_mahaffey-at-yahoo.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
We have a brass coupler with threads to put a Nikon objective on an Olympus nosepiece. Don't know where it came from, but it looks homemade.
We need a few more.
Before asking our machine shop to duplicate it, we were wondering if anybody knows whether we can purchase them.
Thanks. ____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. URL: http://www.aecom.yu.edu/aif/
==============================Original Headers============================== 5, 30 -- From cammer-at-aecom.yu.edu Mon Jan 8 09:04:27 2007 5, 30 -- Received: from mx1.aecom.yu.edu (mx1.aecom.yu.edu [129.98.1.51]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l08F4R3n003670 5, 30 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 09:04:27 -0600 5, 30 -- Received: from draco.aecom.yu.edu (draco.aecom.yu.edu [129.98.1.160]) 5, 30 -- by mx1.aecom.yu.edu (Postfix) with ESMTP id CABCA9F0015 5, 30 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 10:04:26 -0500 (EST) 5, 30 -- Received: from draco.aecom.yu.edu (unknown [127.0.0.1]) 5, 30 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id EB05D8B4003 5, 30 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 09:53:57 -0500 (EST) 5, 30 -- X-AuditID: 816201a0-9f8c8bb000000505-bb-45a25b05cc87 5, 30 -- Received: from post.aecom.yu.edu (post.aecom.yu.edu [129.98.1.100]) 5, 30 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id B48FE718002 5, 30 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 09:53:57 -0500 (EST) 5, 30 -- Received: from AIF3.aecom.yu.edu (aif3.aif.aecom.yu.edu [129.98.80.70]) 5, 30 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 5, 30 -- (No client certificate requested) 5, 30 -- by post.aecom.yu.edu (Postfix) with ESMTP id 6038026 5, 30 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 10:04:26 -0500 (EST) 5, 30 -- Message-Id: {7.0.1.0.2.20070108100226.0532b168-at-aecom.yu.edu} 5, 30 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 5, 30 -- Date: Mon, 08 Jan 2007 10:04:28 -0500 5, 30 -- To: microscopy-at-microscopy.com 5, 30 -- From: Michael Cammer {cammer-at-aecom.yu.edu} 5, 30 -- Subject: Nikon to Olympus objective converter 5, 30 -- In-Reply-To: {200701052049.l05KnBOD018011-at-ns.microscopy.com} 5, 30 -- References: {200701052049.l05KnBOD018011-at-ns.microscopy.com} 5, 30 -- Mime-Version: 1.0 5, 30 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 5, 30 -- X-Brightmail-Tracker: AAAAAA== ==============================End of - Headers==============================
You should be able to see them fairly easily at 10-40x, if they are there, as they are often over 200um long - you need a decent microscope though. You can chemically test for their presence using a chemical card. They need 70% humidity and food to thrive - so an old unused airing cupboard pillow might not provide any living specimens - perhaps try a mattress that's in use. Other than allergenic dust problems with their faeces & dead mites (which can be severe) they are harmless.
A 4 to 120x stereo microscope should also work well, although they are a little translucent, and they are odd looking beasties. Rather than repeat other on-line info, have a look at:
Dr Keith J Morris Imaging Facility Manager Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
email: keith.Morris-at-ucl.ac.uk
-----Original Message----- X-from: Lori_mahaffey-at-yahoo.com [mailto:Lori_mahaffey-at-yahoo.com] Sent: 08 January 2007 14:12 To: keith.morris-at-ucl.ac.uk
This Question was submitted to Ask-A-Microscopist by (Lori_mahaffey-at-yahoo.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, January 7, 2007 at 17:14:47 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both Lori_mahaffey-at-yahoo.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
We also decomissioned our darkroom several years ago, since we had only one remaining client that wanted prints, and she only wanted one or two 8x10's per year. Our Durst Labrator (sp?) went to another building and is still in use, I'm told.
I purchased an inkjet, "photo-quality" printer, in case anyone really needed prints, but it was never used and the ink cartridges eventually went bad.
I also paid my darkroom dues over many years. I think every piece of work clothing I owned had developer stains and I always squinted in normal light. I will always have a fond place in my heart for silver-based photography, and may even go back to it someday for the artsy-fartsy part of my life, but I will not shed a tear for it in research work. We will soon replace our old TEM with a film- and digital-capable new one, but I expect that we will go from thousands of sheets of 4489 per year to tens----if that.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: Debby Sherman [mailto:dsherman-at-purdue.edu] Sent: Thursday, January 04, 2007 4:13 PM To: Alan Nicholls Cc: Christopher J. Gilpin; Angela Klaus; Anlee Krupp; Ann Lehman; Anne-Marie Girard; Annette Andrews; Anthony McCormick; Bobbie Schneider; Brigitte Shaw; Bryony James; Chere Petty; Clive Wells; David Dorward; David Hall; Dee Breger; Donald Gantz; Douglas Cromey; Edward Seijo; Elaine Humphrey; Elison Blancaflor; Evelyn York; Miller, F. Scott; Frank Macaluso; Gary Chandler; Geoffrey Williams; Halina Witkiewicz; Hendrik Colijn; Jacob Mey; Jaime Dant; Janice Pennington; Jeffrey Hanson; Jim Conner; John Bozzola; John Curulli; John Gardner; Jon Mulholland; Jonathan Krupp; Joseph Kulik; Judith Drazba; Julie Getz; Karen Kelley; Karen Stevenson; Karl Wendt; Laura Raymond; Leona Cohen-Gould; Longzhou Ma; Louis Kerr; Luisa Dempere; Margaret Bisher; Margaret Sherwood; Marilyn Dunlap; Mark Walters; Melanie Barfels; Michael Goheen; Michelle Ocana; Missy Hazen; Mitchell McCartney; Neelima Shah; Norman Olson; Patricia Connelly; Patricia Jansma; Paula Allan-Wojtas; Philip Oshel; Phillip Russell; Rakesh Bhatnagar; Rebecca Stearns; Richard Gursky; Robin Griffin; Rogelio Pescador; Sardiaa Plaud; Steven Barlow; Stuart McKernan; Thomas Freeman; Thomas Murray; Timothy Maugel; Tina Carvalho; Tsuey-Chen Long; Wendy Salmon; William L'Amoreaux; William Russin; Zsolt Lazar; Anita K. McCauley PhD; Chunfei Li Ph.D.; Earnest W. Truby Ph. D.; Edwina W. Westbrook (Winnie); Gary M. Brown; James Hayden (Jamie); Kenneth L. Tiekotter; Leslie M. Eibest; Mayandi Sivaguru Ph.D. (Shiv); Qian-Chun Yu MB Ph.D.; R. Holland Cheng; S. Kelly Sears Ph.D. B.F.A.; Sousan Abolhassani Ph.D.; Warren Straszheim Ph.D.; Barbara maloney; Ben Fowler; Beverly Giammara; Bill Tivol; Caroline Miller; Chaoying Ni PhD; Christian Mellen; Christine Davis; Donald Robertson; Eduardo Rosa-Molinar; Fred Monson PhD; Greg Ning; Hilary Holloway; Jacqueline Mills; Jan Redick; Jeff Braswell; Jeff Horn; Judy Murphy; Kent McDonald; Kim Christensen; Lois Anderson; Ross Jr, Louis M.; Marc Pypaert; Marilee Sellers; Michael Dunlap; Mike Gemble; Randy Nessler; Tindall, Randy D.; Reinhard Rachel; Richard Schalek; Ron Anderson; Rosemary White; Sara Miller; Steve Chapman; Phillips, Thomas E.
Dear colleeagues:
Anyone have a protocol for the decalcification of adult zebrafish?
Geoff
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 6, 33 -- From mcauliff-at-umdnj.edu Mon Jan 8 09:35:21 2007 6, 33 -- Received: from zix02.umdnj.edu (zix02.UMDNJ.EDU [130.219.34.125]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l08FZLfS004547 6, 33 -- for {microscopy-at-msa.microscopy.com} ; Mon, 8 Jan 2007 09:35:21 -0600 6, 33 -- Received: from zix02.umdnj.edu (ZixVPM [127.0.0.1]) 6, 33 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id BEE984BE2E 6, 33 -- for {microscopy-at-msa.microscopy.com} ; Mon, 8 Jan 2007 10:35:20 -0500 (EST) 6, 33 -- Received: from umdnj.edu (imail.UMDNJ.EDU [130.219.34.129]) 6, 33 -- by zix02.umdnj.edu (Proprietary) with ESMTP id 855C44BE76 6, 33 -- for {microscopy-at-msa.microscopy.com} ; Mon, 8 Jan 2007 10:35:19 -0500 (EST) 6, 33 -- Received: from ([130.219.34.131]) 6, 33 -- by imail.umdnj.edu with ESMTP id KP-BRACD.123713466; 6, 33 -- Mon, 08 Jan 2007 10:35:00 -0500 6, 33 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 6, 33 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 6, 33 -- id {0JBK005012VZK1-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 6, 33 -- for microscopy-at-msa.microscopy.com; Mon, 08 Jan 2007 10:35:00 -0500 (EST) 6, 33 -- Received: from [127.0.0.1] ([10.138.2.123]) 6, 33 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 6, 33 -- 2004)) with ESMTP id {0JBK003FJ3A7Y9-at-Polaris.umdnj.edu} ; Mon, 6, 33 -- 08 Jan 2007 10:34:56 -0500 (EST) 6, 33 -- Date: Mon, 08 Jan 2007 10:36:17 -0500 6, 33 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 6, 33 -- Subject: decalcification of Zebrafish 6, 33 -- To: MicroscopyListserver {microscopy-at-msa.microscopy.com} , 6, 33 -- Histonet {histonet-at-pathology.swmed.edu} 6, 33 -- Message-id: {45A264F1.4000904-at-umdnj.edu} 6, 33 -- MIME-version: 1.0 6, 33 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 6, 33 -- Content-transfer-encoding: 7BIT 6, 33 -- X-Accept-Language: en-us, en 6, 33 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 6, 33 -- Gecko/20040804 Netscape/7.2 (ax) ==============================End of - Headers==============================
Having been trained in classic darkroom B&W procedures back in late 70's/early 80's (TEM 4x5, 35mm (Plus-X, Tri-X), -- and Polaroid Type 55), we decommissioned our darkroom ~8 years ago, having moved to digital TEM and LM imaging two years prior to that. We stocked color film (Kodachrome, Ektachrome, Kodacolor) but that was always sent out for processing.
Basic photographic theory and practice still applies. The only thing that has changed is the workflow, with more emphasis at the point of capture, rather than after-the-fact processing/printing. In fact, we perform very little printing, as 99% of needs are in digital format. Any prints are done with a high end Fuji Pictrography printer (silver halide based digital print system that rivals a classical photograph!)
Also, as long as you understand the technology, an inkjet can equal/exceed a photograph. You must carefully choose the printer & corresponding paper, and ink media. For B&W photography, one should examine quad tone inks (multi-tone black inks).
With digital, there is a learning curve to understand concepts of camera sensors (what the camera is actually recording), image pixels, resolution, how to match the proper amount of digital information for a particular output, and file storage formats (TIF, JPG, etc) and advantages/disadvantages of each. Everything you ever did in the darkroom is right there in your image editor (like the consumer Photoshop Elements or even professional strength Photoshop CS).
Best regards,
Walter F. Bobrowski Sr. Scientist Pfizer Global R&D 2800 Plymouth Rd. Ann Arbor, MI 48105
TEL: 734-622-7814 FAX: 734-622-5718
"The ultimate human freedom is the ability to choose one's attitude in a given set of circumstances." -Viktor Frankl
-----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: Monday, January 08, 2007 10:33 AM To: Bobrowski, Walter
Alan,
We also decomissioned our darkroom several years ago, since we had only one remaining client that wanted prints, and she only wanted one or two 8x10's per year. Our Durst Labrator (sp?) went to another building and is still in use, I'm told.
I purchased an inkjet, "photo-quality" printer, in case anyone really needed prints, but it was never used and the ink cartridges eventually went bad.
I also paid my darkroom dues over many years. I think every piece of work clothing I owned had developer stains and I always squinted in normal light. I will always have a fond place in my heart for silver-based photography, and may even go back to it someday for the artsy-fartsy part of my life, but I will not shed a tear for it in research work. We will soon replace our old TEM with a film- and digital-capable new one, but I expect that we will go from thousands of sheets of 4489 per year to tens----if that.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
Hi,
We had to make a few of these up in our in-shouse shop too. However, Thorlabs now sells RMS to M25 (and reverse) commercially for cheap
cammer-at-aecom.yu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We have a brass coupler with threads to put a Nikon objective on an } Olympus nosepiece. Don't know where it came from, but it looks homemade. } } We need a few more. } } Before asking our machine shop to duplicate it, we were wondering if } anybody knows whether we can purchase them. } } Thanks. } ____________________________________________________________________________ } Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. } URL: http://www.aecom.yu.edu/aif/ } } } ==============================Original Headers============================== } 5, 30 -- From cammer-at-aecom.yu.edu Mon Jan 8 09:04:27 2007 } 5, 30 -- Received: from mx1.aecom.yu.edu (mx1.aecom.yu.edu [129.98.1.51]) } 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l08F4R3n003670 } 5, 30 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 09:04:27 -0600 } 5, 30 -- Received: from draco.aecom.yu.edu (draco.aecom.yu.edu [129.98.1.160]) } 5, 30 -- by mx1.aecom.yu.edu (Postfix) with ESMTP id CABCA9F0015 } 5, 30 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 10:04:26 -0500 (EST) } 5, 30 -- Received: from draco.aecom.yu.edu (unknown [127.0.0.1]) } 5, 30 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id EB05D8B4003 } 5, 30 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 09:53:57 -0500 (EST) } 5, 30 -- X-AuditID: 816201a0-9f8c8bb000000505-bb-45a25b05cc87 } 5, 30 -- Received: from post.aecom.yu.edu (post.aecom.yu.edu [129.98.1.100]) } 5, 30 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id B48FE718002 } 5, 30 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 09:53:57 -0500 (EST) } 5, 30 -- Received: from AIF3.aecom.yu.edu (aif3.aif.aecom.yu.edu [129.98.80.70]) } 5, 30 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) } 5, 30 -- (No client certificate requested) } 5, 30 -- by post.aecom.yu.edu (Postfix) with ESMTP id 6038026 } 5, 30 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 10:04:26 -0500 (EST) } 5, 30 -- Message-Id: {7.0.1.0.2.20070108100226.0532b168-at-aecom.yu.edu} } 5, 30 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 } 5, 30 -- Date: Mon, 08 Jan 2007 10:04:28 -0500 } 5, 30 -- To: microscopy-at-microscopy.com } 5, 30 -- From: Michael Cammer {cammer-at-aecom.yu.edu} } 5, 30 -- Subject: Nikon to Olympus objective converter } 5, 30 -- In-Reply-To: {200701052049.l05KnBOD018011-at-ns.microscopy.com} } 5, 30 -- References: {200701052049.l05KnBOD018011-at-ns.microscopy.com} } 5, 30 -- Mime-Version: 1.0 } 5, 30 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } 5, 30 -- X-Brightmail-Tracker: AAAAAA== } ==============================End of - Headers============================== }
-- --------------------------------------------------------------------------- Justin E. Jureller, Ph.D. Technical Director, IBD NanoBiology Facility, The University of Chicago Gordon Center for Integrative Science, 929 E 57th Street, Chicago, IL 60637 office: GCIS ESB06B lab: GCIS ESB18 phone: (773) 834-3864 fax: (773) 834-1917 email: jureller-at-uchicago.edu web: http://nanobio.uchicago.edu/ ---------------------------------------------------------------------------
==============================Original Headers============================== 6, 18 -- From jureller-at-uchicago.edu Mon Jan 8 11:17:03 2007 6, 18 -- Received: from relay01.uchicago.edu (relay01.uchicago.edu [128.135.12.136]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l08HH2GA026129 6, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 11:17:03 -0600 6, 18 -- Received: from [128.135.35.206] (godard.uchicago.edu [128.135.35.206]) 6, 18 -- by relay01.uchicago.edu (8.13.6.20060614/8.12.9) with ESMTP id l08HH2TI011530 6, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 11:17:02 -0600 (CST) 6, 18 -- Message-ID: {45A27C94.3090200-at-uchicago.edu} 6, 18 -- Date: Mon, 08 Jan 2007 11:17:08 -0600 6, 18 -- From: j jureller {jureller-at-uchicago.edu} 6, 18 -- User-Agent: Thunderbird 1.5.0.9 (Windows/20061207) 6, 18 -- MIME-Version: 1.0 6, 18 -- To: Microscopy-at-microscopy.com 6, 18 -- Subject: Re: [Microscopy] Nikon to Olympus objective converter 6, 18 -- References: {200701081509.l08F97Vd011986-at-ns.microscopy.com} 6, 18 -- In-Reply-To: {200701081509.l08F97Vd011986-at-ns.microscopy.com} 6, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
try B&H Photo-Video http://www.bhphotovideo.com/bnh/controller/home?O=NavBar&A=FetchChildren&Q=&ci=9824 , check "Lens to Body Adapters" and "Lens Adapters and Mounts" under "General Lens Accessories".
Even if they don't have Olympus body to Nikon lens adapter, you may combine necessary parts from 2 adapters, these are cheap.
Vitaly Feingold SIA 2773 Heath Line Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com ----- Original Message ----- X-from: {cammer-at-aecom.yu.edu} To: {vitalylazar-at-att.net} Sent: Monday, January 08, 2007 10:05 AM
never mind, I miss-understood the posting.
Vitaly Feingold SIA 2773 Heath Line Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com ----- Original Message ----- X-from: {vitalylazar-at-att.net} To: {vitalylazar-at-att.net} Sent: Monday, January 08, 2007 12:23 PM
We have an Olympus IX 70 that is about six years old that we want to rebuild for a custom laser application.
There are two optics in the light path we need to identify. We would greatly appreciate if anybody knows the answer to please let us know. (The alternative it to disassemble the microscope completely which we'd rather not do.)
The light path from the sample to the detector through the side port is as follows:
Objective lens to window to lens to prism to window. We need to identify the lens (B) and the prism (C). B and C are diagrammed at http://cammer.net/temp/IX70question/
Is the lens what Olympus calls the "tube lens"? What focal length is the lens? How far into the UV and into the IR does the lens pass } 70%?
Similarly, for the prism, how far into the UV and into the IR does the glass pass } 70%?
Thanks!
-Michael ____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. URL: http://www.aecom.yu.edu/aif/
==============================Original Headers============================== 9, 28 -- From cammer-at-aecom.yu.edu Mon Jan 8 15:14:55 2007 9, 28 -- Received: from mx1.aecom.yu.edu (mx1.aecom.yu.edu [129.98.1.51]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l08LEsvP002842 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 15:14:54 -0600 9, 28 -- Received: from draco.aecom.yu.edu (draco.aecom.yu.edu [129.98.1.160]) 9, 28 -- by mx1.aecom.yu.edu (Postfix) with ESMTP id 621A39F0072 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 16:14:54 -0500 (EST) 9, 28 -- Received: from draco.aecom.yu.edu (unknown [127.0.0.1]) 9, 28 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 5531D8B4002 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 16:04:24 -0500 (EST) 9, 28 -- X-AuditID: 816201a0-9da69bb000000505-eb-45a2b1d82c74 9, 28 -- Received: from post.aecom.yu.edu (post.aecom.yu.edu [129.98.1.100]) 9, 28 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 1C3E7718002 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 16:04:24 -0500 (EST) 9, 28 -- Received: from AIF3.aecom.yu.edu (aif3.aif.aecom.yu.edu [129.98.80.70]) 9, 28 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 9, 28 -- (No client certificate requested) 9, 28 -- by post.aecom.yu.edu (Postfix) with ESMTP id DBFA326; 9, 28 -- Mon, 8 Jan 2007 16:14:53 -0500 (EST) 9, 28 -- Message-Id: {7.0.1.0.2.20070108161224.026baad0-at-aecom.yu.edu} 9, 28 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 9, 28 -- Date: Mon, 08 Jan 2007 16:14:37 -0500 9, 28 -- To: microscopy-at-microscopy.com 9, 28 -- From: Michael Cammer {cammer-at-aecom.yu.edu} 9, 28 -- Subject: Olympus IX70 technical question 9, 28 -- Mime-Version: 1.0 9, 28 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 9, 28 -- X-Brightmail-Tracker: AAAAAA== ==============================End of - Headers==============================
Have I become a wimp? I cannot face the new year without my TEM digital camera.
Here is the story:
About 4 years ago a PI on campus bought a digital camera for our central campus lab's TEM. She said anyone could use it, no problem. She got a lot of use from it and so did everyone else.
Now she is leaving and wants to take the camera with her, leaving me without one. None of the users who got used to the camera want to go to film, I don't miss the chemical mixing, disposal, and darkroom time. Users really got used to taking their pictures with them at the end of a session on a memory stick instead of waiting half a day to see if anything turned out and then having to figure out a filing system for all their negatives.
What would you do if someone pulled the rug, er, uh, camera, out from under you? I need to convince our Dean that it is really the only way to go now a days. Is that true or have I become too lazy?
There is a chance that we might be able to cut a deal with the owner of the camera if we offer her a fair price for it. It is a Gatan 1K x 1K Bioscan mounted at the 35 mm port. Any guesses on current value? I know it is kind of old and might be obsolete in some circles, but it's the only one I have and I would like to keep it until we can afford something newer. What would be a fair offer to the owner?
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
==============================Original Headers============================== 10, 21 -- From jmkrupp-at-ucsc.edu Mon Jan 8 16:12:03 2007 10, 21 -- Received: from smtp-prod-mx3.ucsc.edu (smtp-prod-mx3.ucsc.edu [128.114.125.45]) 10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l08MC3PU014681 10, 21 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 16:12:03 -0600 10, 21 -- Received: from ucsc.edu (cruzmail-fe2.ucsc.edu [128.114.125.2]) 10, 21 -- by smtp-prod-mx3.ucsc.edu (8.13.8/8.13.8) with ESMTP id l08MBng5007837 10, 21 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jan 2007 14:12:02 -0800 (PST) 10, 21 -- Received: from [128.114.25.227] (account jmkrupp-at-ucsc.edu HELO [128.114.25.227]) 10, 21 -- by silver.ucsc.edu (CommuniGate Pro SMTP 4.3.7) 10, 21 -- with ESMTPA id 95721644 for microscopy-at-microscopy.com; Mon, 08 Jan 2007 14:11:54 -0800 10, 21 -- Mime-Version: 1.0 10, 21 -- Message-Id: {p06230909c1c86f391353-at-[128.114.25.227]} 10, 21 -- Date: Mon, 8 Jan 2007 14:11:53 -0800 10, 21 -- To: microscopy-at-microscopy.com 10, 21 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 10, 21 -- Subject: digital camera withdrawals 10, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 10, 21 -- X-UCSC-EDU-MIMEDefang: Checked 10, 21 -- X-UCSC-EDU-Sender: {jmkrupp-at-ucsc.edu} 10, 21 -- X-UCSC-CATS-MailScanner-From: {jmkrupp-at-ucsc.edu} 10, 21 -- X-Scanned-By: MIMEDefang 2.58 on 128.114.125.45 ==============================End of - Headers==============================
This is somewhat of a solicitation for information from the cryo TEM community. I'm imaging small (small = less than 20 nm) polymeric nanoparticles with cryo TEM, and as always, am striving for the best image possible. I'd like to obtain good (with respect to characteristics such as resolution and signal/noise ratio) images of small, low contrast, spherical particles, to serve as comparisons for images I generate . Journal and book references would be gladly appreciated. Conventional TEM images, with or without staining, would also be very helpful.
Jessica Cervantes Research Chemist Bend Research, Inc 64550 Research Rd Bend, OR 97701 (541) 382-4100 (541) 382-0212 x240 (541) 382-6177 fax
LEGAL NOTICE: This message (and/or any attachments accompanying it) is confidential and proprietary. It is intended for the addressee(s) only. Access to this e-mail by anyone else is unauthorized. If you are not an addressee any disclosure, copying, or distribution of the contents of this e-mail (and any attachments) or any action taken (or not taken) in reliance upon this message is unauthorized and may be unlawful. If you are not an addressee, please contact the sender immediately by calling (541) 382-4100.
==============================Original Headers============================== 5, 14 -- From cervantes-at-bendres.com Tue Jan 9 10:51:07 2007 5, 14 -- Received: from BRIEX04A.bri.local (bc161112.bendcable.com [216.228.161.112]) 5, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l09Gp5Qd030963 5, 14 -- for {microscopy-at-microscopy.org} ; Tue, 9 Jan 2007 10:51:06 -0600 5, 14 -- MIME-Version: 1.0 5, 14 -- Content-Type: text/plain; 5, 14 -- charset="iso-8859-1" 5, 14 -- Subject: Cryo TEM Imaging of Nanoparticles 5, 14 -- Date: Tue, 9 Jan 2007 08:51:03 -0800 5, 14 -- Message-ID: {8943D65F9AD70E4488AD6DE09F15088768C25E-at-BRIEX04A} 5, 14 -- From: "Cervantes, Jessica" {cervantes-at-bendres.com} 5, 14 -- To: {microscopy-at-microscopy.org} 5, 14 -- Content-Transfer-Encoding: 8bit 5, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l09Gp5Qd030963 ==============================End of - Headers==============================
University of Connecticut Institute of Materials Science
Position in Scanning Electron Microscopy and Optical Microscopy
The Institute of Materials Science (IMS) at UConn is an interdisciplinary center with the threefold mission of fostering education, research and outreach in all areas of the materials sciences. The Microscopy Laboratory is a user facility which houses the main research microscopes (optical, SEM and TEM) for the IMS. There is an opening in the Laboratory for a Scanning Electron Microscopy / Optical Microscopy specialist. The appointee will be involved in a range of academic and industrial projects, and will assist in the operation of the Laboratory including performing routine maintenance and training/assisting users of the microscopes.
Candidates should hold a higher degree (MS or PhD) in Materials Science or a related discipline and must have extensive hands-on SEM and optical microscopy experience. Experience in maintenance of electron microscopes, use of transmission electron microscopy, microscopy of soft materials and/or microtomy would also be beneficial. This is a fixed-term appointment and is available from February 1st. Screening of the applications will begin immediately and will continue until the post is filled. Applications from under-represented groups, including minorities, women and persons with disabilities are encouraged.
Interested candidates should send a curriculum vitae and the names of at least three referees with postal addresses, telephone numbers and Email addresses to: Prof. Mark Aindow, Institute of Materials Science, University of Connecticut, 97 North Eagleville Road, Unit 3136, Storrs, CT 06269-3136 USA. Email: m.aindow-at-uconn.edu
==============================Original Headers============================== 5, 22 -- From m.aindow-at-uconn.edu Tue Jan 9 14:31:49 2007 5, 22 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l09KVnTP014869 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 9 Jan 2007 14:31:49 -0600 5, 22 -- Received: from [137.99.20.179] (d20h179.public.uconn.edu [137.99.20.179]) 5, 22 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id l09KViqW010917; 5, 22 -- Tue, 9 Jan 2007 15:31:44 -0500 5, 22 -- User-Agent: Microsoft-Entourage/11.3.2.061213 5, 22 -- Date: Tue, 09 Jan 2007 15:31:48 -0500 5, 22 -- Subject: Postdoctoral Position 5, 22 -- From: Mark Aindow {m.aindow-at-uconn.edu} 5, 22 -- To: {Microscopy-at-MSA.Microscopy.Com} 5, 22 -- Message-ID: {C1C965E4.1623B%m.aindow-at-uconn.edu} 5, 22 -- Thread-Topic: Postdoctoral Position 5, 22 -- Thread-Index: Acc0LS+XbfUTZaAgEdu8nQARJEXN3A== 5, 22 -- Mime-version: 1.0 5, 22 -- Content-type: text/plain; 5, 22 -- charset="US-ASCII" 5, 22 -- Content-transfer-encoding: 7bit 5, 22 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information. 5, 22 -- X-UConn-MailScanner: Found to be clean 5, 22 -- X-UConn-MailScanner-SpamCheck: ==============================End of - Headers==============================
Try staining the polymeric sections/particles with OsO4 at different times, ie., 5, 7, 10min, look at them with the TEM and select the best results.
Ani
---- Original message ---- } Date: Tue, 9 Jan 2007 11:03:32 -0600 } From: cervantes-at-bendres.com } Subject: [Microscopy] Cryo TEM Imaging of Nanoparticles } To: ani.issaian-at-csun.edu } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 4, 29 -- From ani.issaian-at-csun.edu Tue Jan 9 18:13:45 2007 4, 29 -- Received: from plover.csun.edu (plover.csun.edu [130.166.1.24]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0A0DjED002579 4, 29 -- for {microscopy-at-microscopy.org} ; Tue, 9 Jan 2007 18:13:45 -0600 4, 29 -- Received: from puffin.csun.edu (puffin.csun.edu [130.166.1.21]) 4, 29 -- by plover.csun.edu (MOS 3.8.2-GA) 4, 29 -- with ESMTP id DLF31054 4, 29 -- for {microscopy-at-microscopy.org} ; 4, 29 -- Tue, 9 Jan 2007 16:13:44 -0800 (PST) 4, 29 -- Received: from cuckoo.csun.edu (cuckoo.csun.edu [130.166.1.230]) 4, 29 -- by puffin.csun.edu (MOS 3.7.5a-GA) 4, 29 -- with ESMTP id FIS39177 4, 29 -- for {microscopy-at-microscopy.org} ; 4, 29 -- Tue, 9 Jan 2007 16:13:43 -0800 (PST) 4, 29 -- Received: (from cuckoo.csun.edu [130.166.114.202]) 4, 29 -- by cuckoo.csun.edu (MOS 3.8.2-GA) 4, 29 -- with HTTPS/1.1 id AQU44765 (AUTH ami24015); 4, 29 -- Tue, 9 Jan 2007 16:13:43 -0800 (PST) 4, 29 -- From: Ani M Issaian {ani.issaian-at-csun.edu} 4, 29 -- Subject: Re: [Microscopy] Cryo TEM Imaging of Nanoparticles 4, 29 -- To: microscopy-at-microscopy.org 4, 29 -- Reply-To: ani.issaian-at-csun.edu 4, 29 -- X-Mailer: Mirapoint Webmail Direct 3.8.2-GA 4, 29 -- MIME-Version: 1.0 4, 29 -- Content-Type: text/plain; charset=us-ascii 4, 29 -- Content-Transfer-Encoding: 7bit 4, 29 -- Message-Id: {20070109161343.AQU44765-at-cuckoo.csun.edu} 4, 29 -- Date: Tue, 9 Jan 2007 16:13:43 -0800 (PST) 4, 29 -- X-Junkmail-Whitelist: YES (by domain whitelist at plover.csun.edu) ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (connellyps-at-mail.nih.gov) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, January 9, 2007 at 17:33:37 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both connellyps-at-mail.nih.gov as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: connellyps-at-mail.nih.gov Name: Pat Connelly
Organization: NIH
Education: Graduate College
Location: Bethesda, MD
Title: fixation for mitochondria
Question: Dear Fellow Experts,
I am in need of the "best" fixation for mitochondria in mouse and rat muscle and kidney. Past experiments have yeilded OK but not great results using 2.5%glut with and without paraformaldehyde in sodium cacodylate buffer followed by 1% OsO4 and UA block stain. I'd like to be saying "Wow, Look at these!" Any suggestions or references? Today I did a Pub Med search and most of what I found was the same as I have been doing.
Microscopy Technologist Position at The Dow Chemical Company in Freeport, Texas
The Dow Chemical Company, an equal opportunity employer, is seeking applications for an immediate opening for a microscopy technologist. Candidates should have training in light and electron microscopies and related sample preparation methods. The applicant should have a minimum of an Associate Degree in Science. The successful applicant will work under the direction of a Dow Researcher to deliver microscopy related solutions to real world industrial problems. The job focus will be primarily related to microscopy of polymers including; light microscopy, scanning electron microscopy and transmission electron microscopy and soft material sample preparation to include; ultramicrotomy and cryo-ultramicrotomy. The position will be at Dow's Freeport, Texas Analytical Laboratory, which houses modern LM, SEM, TEM, SPM and surface science equipment.
The salary is commensurate with experience. Please send hardcopy resume' to: Dr. John Blackson Building 1897 E29C The Dow Chemical Company Midland, MI 48667
Electronic resume' can be sent to tdominowski-at-dow.com
Best Regards,
Bill William A. Heeschen, Ph.D. Building 1897 E84 The Dow Chemical Company Midland, MI 48667 waheeschen-at-dow.com
Dow is a leader in science and technology, providing innovative chemical, plastic and agricultural products and services to many essential consumer markets. With annual sales of $40 billion, Dow serves customers in 175 countries and a wide range of markets that are vital to human progress: food, transportation, health and medicine, personal and home care, and building and construction, among others. Committed to the principles of sustainable development, Dow and its 43,000 employees seek to balance economic, environmental and social responsibilities. References to "Dow" or the "Company" mean The Dow Chemical Company and its consolidated subsidiaries unless otherwise expressly noted.
==============================Original Headers============================== 8, 23 -- From WAHeeschen-at-dow.com Wed Jan 10 07:53:49 2007 8, 23 -- Received: from mail86.messagelabs.com (mail86.messagelabs.com [216.82.244.115]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0ADrmHG005876 8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Jan 2007 07:53:49 -0600 8, 23 -- X-VirusChecked: Checked 8, 23 -- X-Env-Sender: WAHeeschen-at-dow.com 8, 23 -- X-Msg-Ref: server-13.tower-86.messagelabs.com!1168437227!28046730!1 8, 23 -- X-StarScan-Version: 5.5.10.7; banners=-,-,- 8, 23 -- X-Originating-IP: [216.99.65.27] 8, 23 -- Received: (qmail 19531 invoked from network); 10 Jan 2007 13:53:47 -0000 8, 23 -- Received: from mail7.dow.com (HELO mante88.nam.dow.com) (216.99.65.27) 8, 23 -- by server-13.tower-86.messagelabs.com with SMTP; 10 Jan 2007 13:53:47 -0000 8, 23 -- Received: by mante88.nam.dow.com with Internet Mail Service (5.5.2658.3) 8, 23 -- id {C4JXN6G9} ; Wed, 10 Jan 2007 08:53:46 -0500 8, 23 -- Message-ID: {9FAC91DDE67EF3448238E5E33D3E5AEF05383A-at-USMDLMDOWX026.dow.com} 8, 23 -- From: "Heeschen, Bill (WA)" {WAHeeschen-at-dow.com} 8, 23 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 8, 23 -- Subject: Job Posting: Microscopy technologist for Dow Chemical in Freeport 8, 23 -- , TX 8, 23 -- Date: Wed, 10 Jan 2007 08:53:37 -0500 8, 23 -- MIME-Version: 1.0 8, 23 -- X-Mailer: Internet Mail Service (5.5.2658.3) 8, 23 -- Content-Type: text/plain ==============================End of - Headers==============================
I had problems with Hitachi H800 TEM vacuum sequences now. From start up of Evac, three rough pumps(RP) start to work. RP1 and 2 pump the valve of two diffusion pump to open. RP3 cause the pre evac and a valve of column to open. Then the vacuum level of column and pre evac are just stop at around 8*10^-1 pa, no further progress can be reach.
Please share your idea what may be the problems. Thanks.
Jiaming Zhang
Research Assistant Department of Chem. Eng. & Mater. Sci. Michigan State University Tel: 517-231-8013 Email: zhangj16-at-egr.msu.edu
==============================Original Headers============================== 7, 16 -- From zhangj16-at-msu.edu Wed Jan 10 14:04:17 2007 7, 16 -- Received: from sys30.mail.msu.edu (sys30.mail.msu.edu [35.9.75.130]) 7, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0AK4H5S026401 7, 16 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Jan 2007 14:04:17 -0600 7, 16 -- Received: from zhangj16 by sys30.mail.msu.edu with local (Exim 4.52 #1) 7, 16 -- id 1H4jgG-0007Ed-VX 7, 16 -- for Microscopy-at-microscopy.com; Wed, 10 Jan 2007 15:04:17 -0500 7, 16 -- From: "Jiaming Zhang" {zhangj16-at-msu.edu} 7, 16 -- To: Microscopy-at-microscopy.com 7, 16 -- Subject: Hitachi H800 vacuum problem 7, 16 -- Date: Wed, 10 Jan 2007 15:04:07 -0500 7, 16 -- Mime-Version: 1.0 7, 16 -- Content-Type: text/plain; format=flowed; charset="utf-8" 7, 16 -- Content-Transfer-Encoding: 7bit 7, 16 -- Message-Id: {E1H4jgG-0007Ed-VX-at-sys30.mail.msu.edu} 7, 16 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
It sounds as though your vacuum system has basically the same configuration as the one shown in Figure 9.5 of my book, Vacuum Methods in Electron Microscopy (available from SPI Supplies, M. E. Taylor, Ladd, etc.), except that you have diffusion pumps rather than the turbomolecular pumps shown in this figure. The discussion given of the operation of the system in this figure should give you a pretty good understanding of how your system should operate.
In particular, I suspect that RP3 on your system might serve two functions: 1. it might be used to rough pump the column during an initial pump down operation, and 2. it probably serves to evacuate the specimen chamber during specimen exchange operations. If these assumptions are correct then the valves between RP3 and the column should open during the initial stages of pumpdown, but once a suitable vacuum (about 10-1 Pa) is reached in the column the valves between RP3 and the column should close and remain closed, except during specimen change operations. You might want to check the operation of these valves on your system to be sure that they are operating properly. If they do not close when they should they could cause the column pressure to remain too high, as you describe. If you check with your department's electrician he will have a gadget that he can simply clamp around the wires leading to these valves that will read the current flowing to them, and thus be able to determine when they are activated and when they are not.
If the problem does not involve the RP3 system, then there must be something wrong with the diffusion pumps. Either the valves between them and the column are not opening as they should or the diffusion pumps themselves are not working properly. There are many potential problems with diffusion pumps, too many to discuss here; however, most of them are discussed in considerable detail in Section 5.8 (p. 214) of my book. First check to be sure the valves in these systems are operating properly, next check to be sure the DPs are getting proper electrical power , then check that the flow of cooling water is as it should be. Beyond that you have a really serious problem and probably should call in the Hitachi service representative.
Good luck,
-- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-engin.umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 5, 14 -- From bigelow-at-engin.umich.edu Wed Jan 10 15:13:36 2007 5, 14 -- Received: from srvr22.engin.umich.edu (srvr22.engin.umich.edu [141.213.75.21]) 5, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0ALDZug006727 5, 14 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jan 2007 15:13:36 -0600 5, 14 -- Received: from [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 5, 14 -- by srvr22.engin.umich.edu (8.13.6/8.13.6) with ESMTP id l0ALDYrn016264 5, 14 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jan 2007 16:13:34 -0500 (EST) 5, 14 -- Mime-Version: 1.0 5, 14 -- Message-Id: {p06210200c1cafe775158-at-[141.212.131.221]} 5, 14 -- Date: Wed, 10 Jan 2007 16:13:33 -0500 5, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 5, 14 -- From: Wil Bigelow {bigelow-at-engin.umich.edu} 5, 14 -- Subject: RE: H800 vacuum problem 5, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
The vacuum level stated in your message indicates only roughing pressure. I would check to see if both diffusion pump heaters are hot (don't touch these directly with your hand; see if you can sense warmth by holding your hand an inch or so away from the base of the pump), the sides of the diffusion pumps are cool (confirming you have adequate cooling water; again be cautious), and if all this seems ok, clean your penning head as the vacuum system sequence relies on this reading to continue on to high vacuum pumping and appropriate valving. I think your microscope instruction manual details a way to do this cleaning.
Good luck !
Bob Roberts EM Lab Services, Inc. 449 NW 62nd St. Topeka, Kansas 66617-1780 785.246.1232 voice 785.246.0168 fax www.emlabservices.com
==============================Original Headers============================== 6, 26 -- From emlabservices-at-cox.net Wed Jan 10 16:03:41 2007 6, 26 -- Received: from centrmmtao02.cox.net (centrmmtao02.cox.net [70.168.83.82]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0AM3eWM018806 6, 26 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jan 2007 16:03:41 -0600 6, 26 -- Received: from eastrmimpo01.cox.net ([68.1.16.119]) by centrmmtao02.cox.net 6, 26 -- (InterMail vM.6.01.06.03 201-2131-130-104-20060516) with ESMTP 6, 26 -- id {20070110220340.MQPF25837.centrmmtao02.cox.net-at-eastrmimpo01.cox.net} 6, 26 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jan 2007 17:03:40 -0500 6, 26 -- Received: from EMLabServices ([24.255.213.54]) 6, 26 -- by eastrmimpo01.cox.net with bizsmtp 6, 26 -- id 9a2A1W0081AzDvc0000000; Wed, 10 Jan 2007 17:02:17 -0500 6, 26 -- Message-ID: {006c01c73503$2f0836f0$6400a8c0-at-EMLabServices} 6, 26 -- From: "EM Lab Services" {emlabservices-at-cox.net} 6, 26 -- To: {Microscopy-at-microscopy.com} 6, 26 -- Subject: [Microscopy] Hitachi H800 vacuum problem 6, 26 -- Date: Wed, 10 Jan 2007 16:03:36 -0600 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: text/plain; 6, 26 -- format=flowed; 6, 26 -- charset="iso-8859-1"; 6, 26 -- reply-type=original 6, 26 -- Content-Transfer-Encoding: 7bit 6, 26 -- X-Priority: 3 6, 26 -- X-MSMail-Priority: Normal 6, 26 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3028 6, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 ==============================End of - Headers==============================
Post-Doctoral Fellowship in TEM and Materials Characterization Materials Science and Engineering Department University of Missouri-Rolla
The University of Missouri-Rolla, Department of Materials Science and Engineering, is seeking candidates for a postdoctoral fellowship position in transmission electron microscopy of thin films and hard materials. In addition to experience in TEM sample preparation and analysis, the ability to utilize other materials characterization techniques such as Auger and x-ray photoelectron spectroscopies and scanning electron microscopy is also desired. Experience in the characterization of interfaces is also beneficial, though not required. Candidate must be able to work independently and in a group, have excellent communication skills, and provide guidance to team members. The project is a part of a multi-university research initiative on diamond coatings.
A Ph.D. degree in materials science or a related field is preferred; persons with a MS degree may be acceptable with extensive background in TEM. A minimum of 2 years of experience operating a TEM, analyzing images and electron diffraction results, and preparation of TEM samples is required.
Interested candidates should send a Resume/Curriculum Vitae, Letter of Intent, and References to: Prof. Matt O'Keefe, 302 Materials Research Center, University of Missouri-Rolla, Rolla, MO 65409 USA. Email: mjokeefe-at-umr.edu
==============================Original Headers============================== 6, 32 -- From smiller-at-umr.edu Wed Jan 10 17:13:26 2007 6, 32 -- Received: from smtpout2.cc.umr.edu (smtpout2.cc.umr.edu [131.151.0.94]) 6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0ANDNov031485 6, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Jan 2007 17:13:25 -0600 6, 32 -- Received: (qmail 20051 invoked from network); 10 Jan 2007 23:13:20 -0000 6, 32 -- Received: from scansrv2.cc.umr.edu (131.151.1.114) 6, 32 -- by smtpout-ipvs.cc.umr.edu with SMTP; 10 Jan 2007 23:13:20 -0000 6, 32 -- Received: (qmail 25870 invoked from network); 10 Jan 2007 23:13:19 -0000 6, 32 -- Received: from smtp1.cc.umr.edu (131.151.1.43) 6, 32 -- by scanout-ipvs.cc.umr.edu with SMTP; 10 Jan 2007 23:13:19 -0000 6, 32 -- Received: from umr-exproto2.cc.umr.edu (umr-exproto2.cc.umr.edu [131.151.0.192]) 6, 32 -- by smtp1.cc.umr.edu (8.13.1/8.13.1) with ESMTP id l0ANDJ2V008604 6, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Jan 2007 17:13:19 -0600 6, 32 -- Received: from UMR-CMAIL3.umr.edu ([131.151.1.77]) by umr-exproto2.cc.umr.edu with Microsoft SMTPSVC(6.0.3790.1830); 6, 32 -- Wed, 10 Jan 2007 17:13:19 -0600 6, 32 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 32 -- Content-class: urn:content-classes:message 6, 32 -- MIME-Version: 1.0 6, 32 -- Content-Type: text/plain; 6, 32 -- charset="iso-8859-1" 6, 32 -- Subject: TEM postdoctoral position 6, 32 -- Date: Wed, 10 Jan 2007 17:13:18 -0600 6, 32 -- Message-ID: {0FE4BF38C44B644F8E12B86EFC392F86031EAA-at-UMR-CMAIL3.umr.edu} 6, 32 -- X-MS-Has-Attach: 6, 32 -- X-MS-TNEF-Correlator: 6, 32 -- Thread-Topic: TEM postdoctoral position 6, 32 -- Thread-Index: Acc1DOmyuPrjYjTFEce63WJENA16mA== 6, 32 -- From: "Miller, F. Scott" {smiller-at-umr.edu} 6, 32 -- To: {Microscopy-at-microscopy.com} 6, 32 -- X-OriginalArrivalTime: 10 Jan 2007 23:13:19.0166 (UTC) FILETIME=[EA6425E0:01C7350C] 6, 32 -- Content-Transfer-Encoding: 8bit 6, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0ANDNov031485 ==============================End of - Headers==============================
If anyone on the list is using the HKL ebsd system, please contact me at johnf-at-geology.wisc.edu I have a question regarding the quality of backscatter vs forescatter image display from the detectors sitting above and below the camera.
Thanks.
John
==============================Original Headers============================== 3, 24 -- From johnf-at-geology.wisc.edu Thu Jan 11 05:16:37 2007 3, 24 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0BBGbkp022802 3, 24 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jan 2007 05:16:37 -0600 3, 24 -- Received: from localhost (localhost [127.0.0.1]) 3, 24 -- by localhost (Postfix) with ESMTP id BCBF520D1F 3, 24 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jan 2007 05:16:36 -0600 (CST) 3, 24 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) 3, 24 -- by localhost (geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 3, 24 -- with ESMTP id 02001-04 for {microscopy-at-microscopy.com} ; 3, 24 -- Thu, 11 Jan 2007 05:16:31 -0600 (CST) 3, 24 -- Received: from [192.168.0.122] (dyn-192-16.vpn.wisc.edu [146.151.192.16]) 3, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 3, 24 -- (No client certificate requested) 3, 24 -- by ice.geology.wisc.edu (Postfix) with ESMTP id 9664720D07 3, 24 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jan 2007 05:16:31 -0600 (CST) 3, 24 -- Mime-Version: 1.0 3, 24 -- Message-Id: {p06210202c1cbcc7bd7f3-at-[192.168.0.122]} 3, 24 -- Date: Thu, 11 Jan 2007 07:46:27 -0330 3, 24 -- To: microscopy-at-microscopy.com 3, 24 -- From: John Fournelle {johnf-at-geology.wisc.edu} 3, 24 -- Subject: HKL ebsd image question 3, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 24 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu ==============================End of - Headers==============================
If what you mean by "best fixation" is a fixation which does not allow artifacts or difformations of the structure, apart from cryo-fixation I think that your fixation is optimal (and is pretty classical). Personally I have a preference for the addition of a low concentration of formaldehyde (2%) because it penetrates faster than glutar in the tissue. It acts as a sort of "pre-fixative" before the glutar comes and stabilizes the structures.
If you mean "increasing contrasting of the membranes", you could consider using ferrocyanide (which I tried successfully) and/or tannic acid (which I didn't try but is well documented).
I found this reference for you but there are probably others: Berryman MA, et al (1992) Effects of tannic acid on antigenicity and membrane contrast in ultrastructural immunocytochemistry. J Histochem Cytochem 40, 6, 845-857
Regards or LG as they say here in Austria
Stephane
--- connellyps-at-mail.nih.gov wrote:
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==============================Original Headers============================== 14, 20 -- From nizets2-at-yahoo.com Thu Jan 11 09:05:42 2007 14, 20 -- Received: from web37404.mail.mud.yahoo.com (web37404.mail.mud.yahoo.com [209.191.91.136]) 14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0BF5fSZ005801 14, 20 -- for {microscopy-at-microscopy.com} ; Thu, 11 Jan 2007 09:05:42 -0600 14, 20 -- Received: (qmail 63482 invoked by uid 60001); 11 Jan 2007 15:05:39 -0000 14, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 14, 20 -- s=s1024; d=yahoo.com; 14, 20 -- h=Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 14, 20 -- b=o5tVbGn23hSH3z6CRVLjoyWzV9top0IRPXe4qip8oENP+LnVHbJP57XOFCof212p1Im11YjpytjOf23oojveAba5ip3SQjwKsxfioVmRLAcvjgQDw0Aj+eg8kRUpq3REL9enFbZNtV/pQr0HWktUlPrCiohcY16p5ZHr+VAo6ic=; 14, 20 -- Received: from [80.122.101.102] by web37404.mail.mud.yahoo.com via HTTP; Thu, 11 Jan 2007 07:05:39 PST 14, 20 -- Date: Thu, 11 Jan 2007 07:05:39 -0800 (PST) 14, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 14, 20 -- Subject: Re: [Microscopy] AskAMicroscopist: fixation for mitochondria 14, 20 -- To: connellyps-at-mail.nih.gov 14, 20 -- Cc: microscopy-at-microscopy.com 14, 20 -- In-Reply-To: {200701100139.l0A1dAlE022824-at-ns.microscopy.com} 14, 20 -- MIME-Version: 1.0 14, 20 -- Content-Type: text/plain; charset=iso-8859-1 14, 20 -- Content-Transfer-Encoding: 8bit 14, 20 -- Message-ID: {729801.62970.qm-at-web37404.mail.mud.yahoo.com} ==============================End of - Headers==============================
I agree with Stephane, there is nothing wrong with the fixative you are using. If more contrast is what you seek you could also try a post-fix or mordant in potassium dichromate. Substituting Dalton's chrome-osmium fix for your regular osmium post-fix might be worth a try. Alternatively, you could try a "light" fix followed by histochemistry to demo mitochondrial enzymes, they post fix with osmium. This would be more work.
Geoff
connellyps-at-mail.nih.gov wrote:
} } Email: connellyps-at-mail.nih.gov } Name: Pat Connelly } } Organization: NIH } } Education: Graduate College } } Location: Bethesda, MD } } Title: fixation for mitochondria } } Question: Dear Fellow Experts, } } I am in need of the "best" fixation for mitochondria in mouse and rat muscle and kidney. Past experiments have yeilded OK but not great results using 2.5%glut with and without paraformaldehyde in sodium cacodylate buffer followed by 1% OsO4 and UA block stain. I'd like to be saying "Wow, Look at these!" } Any suggestions or references? Today I did a Pub Med search and most of what I found was the same as I have been doing. } } Thanks, } Pat Connelly } connellyps-at-mail.nih.gov } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 10, 12 -- From zaluzec-at-ultra5.microscopy.com Tue Jan 9 19:35:00 2007 } 10, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 10, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0A1Yxi3014862 } 10, 12 -- for {microscopy-at-microscopy.com} ; Tue, 9 Jan 2007 19:35:00 -0600 } 10, 12 -- Mime-Version: 1.0 } 10, 12 -- X-Sender: zaluzec-at-ultra5.microscopy.com (Unverified) } 10, 12 -- Message-Id: {p06110401c1c9f33021fc-at-[206.69.208.22]} } 10, 12 -- Date: Tue, 9 Jan 2007 19:34:58 -0600 } 10, 12 -- To: microscopy-at-microscopy.com } 10, 12 -- From: connellyps-at-mail.nih.gov (by way of Ask-A-Microscopist) } 10, 12 -- Subject: AskAMicroscopist: fixation for mitochondria } 10, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== } } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 9, 35 -- From mcauliff-at-umdnj.edu Thu Jan 11 09:29:07 2007 9, 35 -- Received: from zix04.umdnj.edu (zix04.UMDNJ.EDU [130.219.34.127]) 9, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0BFT6Ri017223 9, 35 -- for {microscopy-at-msa.microscopy.com} ; Thu, 11 Jan 2007 09:29:06 -0600 9, 35 -- Received: from zix04.umdnj.edu (ZixVPM [127.0.0.1]) 9, 35 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 395204BEB8 9, 35 -- for {microscopy-at-msa.microscopy.com} ; Thu, 11 Jan 2007 10:29:06 -0500 (EST) 9, 35 -- Received: from umdnj.edu (imail.UMDNJ.EDU [130.219.34.129]) 9, 35 -- by zix04.umdnj.edu (Proprietary) with ESMTP id 8AF234BECD 9, 35 -- for {microscopy-at-msa.microscopy.com} ; Thu, 11 Jan 2007 10:29:01 -0500 (EST) 9, 35 -- Received: from ([130.219.34.131]) 9, 35 -- by imail.umdnj.edu with ESMTP id KP-BRACD.124903795; 9, 35 -- Thu, 11 Jan 2007 10:27:54 -0500 9, 35 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 9, 35 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 9, 35 -- id {0JBP00F01MQTBQ-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 9, 35 -- for microscopy-at-msa.microscopy.com; Thu, 11 Jan 2007 10:27:53 -0500 (EST) 9, 35 -- Received: from [127.0.0.1] ([10.138.2.123]) 9, 35 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 9, 35 -- 2004)) with ESMTP id {0JBP00C2JMYA9J-at-Polaris.umdnj.edu} ; Thu, 9, 35 -- 11 Jan 2007 10:27:47 -0500 (EST) 9, 35 -- Date: Thu, 11 Jan 2007 10:29:07 -0500 9, 35 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 9, 35 -- Subject: Re: fixation for mitochondria 9, 35 -- In-reply-to: {200701100136.l0A1aDAo016699-at-ns.microscopy.com} 9, 35 -- To: connellyps-at-mail.nih.gov, 9, 35 -- MicroscopyListserver {microscopy-at-msa.microscopy.com} 9, 35 -- Message-id: {45A657C3.4040907-at-umdnj.edu} 9, 35 -- MIME-version: 1.0 9, 35 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 9, 35 -- Content-transfer-encoding: 7BIT 9, 35 -- X-Accept-Language: en-us, en 9, 35 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 9, 35 -- Gecko/20040804 Netscape/7.2 (ax) 9, 35 -- References: {200701100136.l0A1aDAo016699-at-ns.microscopy.com} ==============================End of - Headers==============================
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Email: sshroff-at-bmrn.com Name: Shilpa
Organization: BioMarin
Title-Subject: [Filtered] peptide diffusion
Question: Hi -
I am looking at antigen presentation on the surface of a cell line. I am first incubating the cell with a protein (for about 2hrs). The cells are fixed after the incubation in 4% paraformaldehyde, permeabilized in TritonX.
If my protein were fragmented, would I have to worry about loosing the small peptides even though I fixed in paraformaldehyde? I am staining the protien with a fluorescent polyclonal to check its internalization
One other concern would be that your protein was cross-linked to another protein that was then extracted by the Triton X-100.
At 06:17 PM 01/11/07, you wrote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
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Email: almecid-at-tcd.ie Name: Dorothee
Organization: Trinity College Dublin
Title-Subject: [Filtered] TEM grids
Question: Can carbon TEM grids withstand 450 degrees in a furnace?
I consider myself pretty skilled in Photoshop but there is one simple thing I don't know how to do and it drives me crazy. I have searched the Photoshop online help and other websites to no avail. I want to keep the images anchored to the Photoshop screen frame. The problem is that I start to zoom an image and it grows to cover the menu bar. This makes subsequent manipulation tough. I am sure there is just some simple thing to toggle but I can't find it. I use Photoshop CS2 (version 9). thanks for any tips you have. tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
Select the magnifying glass icon. In the options bar that appears across the top of your screen, uncheck the "resize images to fit" item. When you zoom the image the window in which it appears will not change size or position.
On Jan 12, 2007, at 11:01 AM, phillipst-at-missouri.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } I consider myself pretty skilled in Photoshop but there is one } simple thing } I don't know how to do and it drives me crazy. I have searched the } Photoshop online help and other websites to no avail. I want to } keep the } images anchored to the Photoshop screen frame. The problem is that } I start } to zoom an image and it grows to cover the menu bar. This makes } subsequent } manipulation tough. I am sure there is just some simple thing to } toggle but } I can't find it. I use Photoshop CS2 (version 9). thanks for any } tips you } have. tom } } } } Thomas E. Phillips, PhD } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } } 573-882-4712 (office) } 573-882-0123 (fax) } PhillipsT-at-missouri.edu } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } }
==============================Original Headers============================== 7, 22 -- From DrJohnRuss-at-aol.com Fri Jan 12 10:05:40 2007 7, 22 -- Received: from imo-d05.mx.aol.com (imo-d05.mx.aol.com [205.188.157.37]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0CG5dBk004437 7, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 12 Jan 2007 10:05:39 -0600 7, 22 -- Received: from DrJohnRuss-at-aol.com 7, 22 -- by imo-d05.mx.aol.com (mail_out_v38_r7.6.) id f.c14.e14d6c9 (52468); 7, 22 -- Fri, 12 Jan 2007 11:05:32 -0500 (EST) 7, 22 -- Received: from [192.168.123.187] (cpe-065-190-140-239.nc.res.rr.com [65.190.140.239]) by ciaaol-d09.mx.aol.com (v114.2) with ESMTP id MAILCIAAOLD097-ccf445a7b1c931f; Fri, 12 Jan 2007 11:05:30 -0500 7, 22 -- In-Reply-To: {200701121601.l0CG1LCo030612-at-ns.microscopy.com} 7, 22 -- References: {200701121601.l0CG1LCo030612-at-ns.microscopy.com} 7, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 7, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 22 -- Message-Id: {3EF10D56-909A-4B3D-91CA-0CAD4B91154B-at-AOL.com} 7, 22 -- Cc: microscopy-at-msa.microscopy.com 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- From: John Russ {DrJohnRuss-at-aol.com} 7, 22 -- Subject: Re: [Microscopy] Photoshop question 7, 22 -- Date: Fri, 12 Jan 2007 11:05:25 -0500 7, 22 -- To: phillipst-at-missouri.edu 7, 22 -- X-Mailer: Apple Mail (2.752.2) 7, 22 -- X-AOL-IP: 65.190.140.239 7, 22 -- X-Spam-Flag: NO ==============================End of - Headers==============================
Does anyone have a protocol for effective infiltration of lenses encased in retinas? Pretty please?? We have tried extended infiltration times (up to three days) and microwave-assisted infiltrations with Epon/Spurrs and pure Spurrs and have still not achieved good infiltration. There are holes on either side of the retinas to help solutions move in and out, but the lenses are still crumbling during sectioning. The retinas are okay.
I just know somebody's out there who can help with this......
Thanks much.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 7, 23 -- From TindallR-at-missouri.edu Fri Jan 12 10:18:55 2007 7, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0CGItPZ019583 7, 23 -- for {microscopy-at-microscopy.com} ; Fri, 12 Jan 2007 10:18:55 -0600 7, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 7, 23 -- Fri, 12 Jan 2007 10:18:54 -0600 7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="us-ascii" 7, 23 -- Subject: TEM: Infiltration of lens 7, 23 -- Date: Fri, 12 Jan 2007 10:18:54 -0600 7, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68CD1-at-UM-XMAIL08.um.umsystem.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- Thread-Topic: TEM: Infiltration of lens 7, 23 -- Thread-Index: Acc2ZVpHGt3f/kCtSteTmqnj0MoVsw== 7, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 7, 23 -- To: {microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 12 Jan 2007 16:18:54.0545 (UTC) FILETIME=[5ABF9410:01C73665] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0CGItPZ019583 ==============================End of - Headers==============================
National Institute for Biological Standards and Control
Hertfordshire, UK
Post-doctoral Researcher
Division of Cell Biology and Imaging
As part of a DTI initiative in Regenerative Medicine Technologies a three year post-doctoral research position has become available. The project is part of a consortium bringing together strengths in advanced imaging, robotic microwell-based engineering and combinatorial techniques. The programme aims to create a leading technology for quickly and efficiently examining large numbers of linked variables, addressing a central issue of regenerative medicine; the capacity to reproducibly control the differentiation of a high proportion of stem cells. The successful candidate will undertake studies to investigate differentiation of cells and propose solutions to the technical challenges for scaling the patented concept. Work will require development and study of differentiation systems . Interaction between cells, the cells and carrier beads, effects of culture conditions, environment, differentiation media and the functional capacity of cells in biological assays will be considered. We are seeking an enthusiastic post-doctoral scientist capable of pursuing productive research in molecular cell biology. You will have a Life Science PhD or appropriate subject with at least 3 years experience in microscopy in a research environment. It is essential you have experience of confocal and/or electron microscopy. In addition, you will require experience of one or more: immuno-gold/fluorescent, confocal, live cell, cryo-SEM or cryo-TEM techniques. You will be based in the recently refurbished imaging facility equipped with new high resolution electron microscopes and supporting specimen preparation equipment.
Salary will be within Pay Band E on a scale from £24,815 up to £28,478, dependent on experience.
Please see our website www.nibsc.ac.uk for a full job description.
To apply, please submit a full Curriculum Vitae (2 copies), including the names and addresses of two referees, to the Human Resources Department, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG , or email HR-at-nibsc.ac.uk Closing date for applications is 1 February 2007. Please quote reference SU.CB.567 clearly on your application.
==============================Original Headers============================== 11, 25 -- From RFleck-at-nibsc.ac.uk Fri Jan 12 10:36:24 2007 11, 25 -- Received: from jack.nibsc.ac.uk (jack.nibsc.ac.uk [212.219.216.54]) 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0CGaOHi030862 11, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 12 Jan 2007 10:36:24 -0600 11, 25 -- Received: from postbox.nibsc.ac.uk ([212.219.216.15] helo=postbox.ad.nibsc.ac.uk) 11, 25 -- by jack.nibsc.ac.uk with esmtp (Exim 4.63) 11, 25 -- (envelope-from {RFleck-at-nibsc.ac.uk} ) 11, 25 -- id 1H5PO3-0002OS-Pp 11, 25 -- for Microscopy-at-microscopy.com; Fri, 12 Jan 2007 16:36:15 +0000 11, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 25 -- Content-class: urn:content-classes:message 11, 25 -- MIME-Version: 1.0 11, 25 -- Content-Type: text/plain; 11, 25 -- charset="iso-8859-1" 11, 25 -- Subject: Postdoctoral Research Opportunity at NIBSC 11, 25 -- Date: Fri, 12 Jan 2007 16:37:18 -0000 11, 25 -- Message-ID: {0DBB443159C8DA4C83E7B8B6546FC4370520AA68-at-postbox.ad.nibsc.ac.uk} 11, 25 -- X-MS-Has-Attach: 11, 25 -- X-MS-TNEF-Correlator: 11, 25 -- Thread-Topic: Postdoctoral Research Opportunity at NIBSC 11, 25 -- Thread-Index: Acc2Z+ynoEP81rtpTj6tLL+f0lJ3bA== 11, 25 -- From: "Roland Fleck" {RFleck-at-nibsc.ac.uk} 11, 25 -- To: {Microscopy-at-microscopy.com} 11, 25 -- Content-Transfer-Encoding: 8bit 11, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0CGaOHi030862 ==============================End of - Headers==============================
Hi, I am new at using the TEM and preparing my adherent culture cells for this type of imaging so am hoping that someone may have experience with this unusual artifact that showed up in some of my cells. I had 2 sets of cells. One set was untreated and the others were treated with membrane permeate pseudosubstrate peptides and or nocodazole. The cells that were not treated in any way appeared normal upon imaging but the cells that had been treated had fiber-like artifacts throughout the cytoplasm and nuclei. The "fibers" were localized mainly within the cells and appeared to be consistently 0.1umin length. I am assuming the artifact is originating from the peptides but was wondering if anyone else has had this experience. I also repeated the post staining process with new sections to rule out any problems with lead citrate or uranyl acetate precipitants but had the same problem. Thanks for any advice. Page
Page Baluch Arizona State University/SoLS P.O. Box 4601 Tempe, AZ 85287-4601 Lab: 480-965-7011 Fax: 480-965-7599
==============================Original Headers============================== 3, 22 -- From pbaluch-at-imap3.asu.edu Fri Jan 12 11:41:28 2007 3, 22 -- Received: from epo-int1.asu.edu (epo-int1.asu.edu [129.219.187.20]) 3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0CHfR3c010746 3, 22 -- for {microscopy-at-microscopy.com} ; Fri, 12 Jan 2007 11:41:28 -0600 3, 22 -- Received: from webmail2.asu.edu (webmail2.asu.edu [129.219.117.231]) 3, 22 -- by epo-int1.asu.edu (Switch-3.1.8/Switch-3.1.7/asu-postoffice-prod) with ESMTP id l0CHewwh028440 3, 22 -- for {microscopy-at-microscopy.com} ; Fri, 12 Jan 2007 10:40:58 -0700 3, 22 -- Received: (from emma-at-localhost) 3, 22 -- by webmail2.asu.edu (8.12.9/8.12.9/Submit) id l0CHfMsW011067 3, 22 -- for microscopy-at-microscopy.com; Fri, 12 Jan 2007 10:41:23 -0700 3, 22 -- X-Authentication-Warning: webmail2.asu.edu: emma set sender to pbaluch-at-imap3.asu.edu using -f 3, 22 -- To: microscopy-at-microscopy.com 3, 22 -- Subject: TEM artifact ? 3, 22 -- Message-ID: {1168623682.45a7c842ea514-at-webmail.asu.edu} 3, 22 -- Date: Fri, 12 Jan 2007 10:41:22 -0700 (MST) 3, 22 -- From: PAGE.BALUCH-at-asu.edu 3, 22 -- MIME-Version: 1.0 3, 22 -- Content-Type: text/plain; charset=ISO-8859-1 3, 22 -- Content-Transfer-Encoding: 8bit 3, 22 -- User-Agent: IMP/PHP IMAP webmail program 2.2.3 3, 22 -- X-Originating-IP: 149.169.133.32 3, 22 -- X-Virus-Scanned: by amavisd-new ==============================End of - Headers==============================
On Jan 12, 2007, at 6:08 AM, almecid-at-tcd.ie wrote:
} Question: Can carbon TEM grids withstand 450 degrees in a furnace? } Dear Dorothee, Carbon can certainly withstand 450 C, so if the grid itself is made of carbon, the answer should be "yes", assuming that there are no problems with heating or cooling at a rate such that thermal stresses are not introduced. Carbon films on metal grids could be a different story, however. The large difference between the coefficients of expansion of metal and carbon will very likely cause problems. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Fri Jan 12 11:45:49 2007 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0CHjnIu016042 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 12 Jan 2007 11:45:49 -0600 4, 22 -- Received: from water-dog.caltech.edu (water-dog [192.168.1.26]) 4, 22 -- by wood-ox-postvirus (Postfix) with ESMTP 4, 22 -- id 86AF82EF5D; Fri, 12 Jan 2007 09:45:43 -0800 (PST) 4, 22 -- Received: from [192.168.159.233] (jpix-01.caltech.edu [131.215.2.133]) 4, 22 -- by wood-ox.its.caltech.edu (Postfix) with ESMTP 4, 22 -- id 470BA2EF19; Fri, 12 Jan 2007 09:45:42 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v624) 4, 22 -- In-Reply-To: {200701121408.l0CE8ujR016675-at-ns.microscopy.com} 4, 22 -- References: {200701121408.l0CE8ujR016675-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {261ad5a527abf386fcf548bbf0241ba5-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] viaWWW: Carbon Grids and Furnace 4, 22 -- Date: Fri, 12 Jan 2007 09:53:11 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com, almecid-at-tcd.ie 4, 22 -- X-Mailer: Apple Mail (2.624) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.3.3 ==============================End of - Headers==============================
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Email: ian.anderson-at-nist.gov Name: Ian Anderson
Organization: NIST
Title-Subject: [Filtered] Microbeam Analysis Society (MAS) 2007 Topical Workshop
Question: Microbeam Analysis Society (MAS) 2007 Topical Workshop Hyperspectral Imaging II Advanced Measurement Laboratory (AML) May 1 ñ 4, 2007 ? NIST, Gaithersburg, MD, USA
Special provisions for students and Special provisions for students and postdocs postdocs!
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Email: cosandey-at-rci.rutgers.edu Name: Fred Cosandey
Organization: Rutgers University
Title-Subject: [Filtered] PostDoc Research Associate Position
Question: Research Associate Position Transmission Electron Microscopy
The Department of Materials Science and Engineering at Rutgers University is currently seeking a candidate for a Research Associate position in Transmission Electron Microscopy. The successful candidate should have a PhD degree in Materials Science or related discipline and have extensive experience in the use of transmission electron microscope including HAADF, GIF and EELS spectroscopy techniques. Prior experience with JEOL 2010F STEM would be preferable. Specific projects involve STEM analysis of nanostructured electrode materials used in Li-Ion batteries, including EELS determination of valence state and structural as well as chemical analysis during Li-induced phase transformations. Also, chemical and electronic structure determination of interfaces in oxides and carbide systems.
The appointment will be for one year and may be renewable for subsequent years. Interested candidate should send their CV with three references to:
Professor Frederic Cosandey Department of Materials Science and Engineering Rutgers University 607 Taylor Rd. Piscataway, NJ 08854-8065, cosandey-at-rci.rutgers.edu, 732-445-4942 (phone), 732-445-5977 (fax)
Rutgers University is an equal opportunity/affirmative action employer.
} Special provisions for students and Special provisions for } students and postdocs postdocs! } } } http://www.microbeamanalysis.org/workshops/HI /workshops/HI-II }
==============================Original Headers============================== 5, 21 -- From michael-at-shaffer.net Sat Jan 13 06:09:16 2007 5, 21 -- Received: from n034.sc1.he.tucows.com (smtpout1433.sc1.he.tucows.com [64.97.157.133]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0DC9GvD018476 5, 21 -- for {Microscopy-at-microscopy.com} ; Sat, 13 Jan 2007 06:09:16 -0600 5, 21 -- Received: from rarewolf (205.251.83.78) by n034.sc1.he.tucows.com (7.2.069.1) (authenticated as michael-at-shaffer.net) 5, 21 -- id 45A8B00F00003B89; Sat, 13 Jan 2007 12:09:06 +0000 5, 21 -- From: "michael shaffer" {michael-at-shaffer.net} 5, 21 -- To: {ian.anderson-at-nist.gov} 5, 21 -- Cc: "MSA Microscopy list" {Microscopy-at-microscopy.com} 5, 21 -- References: {200701130000.l0D00QeE007702-at-ns.microscopy.com} 5, 21 -- Subject: RE: [Microscopy] viaWWW: Microbeam Analysis Society (MAS) 2007 Topical Workshop 5, 21 -- Date: Sat, 13 Jan 2007 08:37:05 -0330 5, 21 -- Message-ID: {005201c7370b$58db3f40$4701a8c0-at-rarewolf} 5, 21 -- MIME-Version: 1.0 5, 21 -- Content-Type: text/plain; 5, 21 -- charset="US-ASCII" 5, 21 -- Content-Transfer-Encoding: 7bit 5, 21 -- X-Mailer: Microsoft Office Outlook 11 5, 21 -- In-Reply-To: {200701130000.l0D00QeE007702-at-ns.microscopy.com} 5, 21 -- Thread-Index: Acc2pZAxXxlbkOJ4SA6cOAtSV283HAAZYHmA 5, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 ==============================End of - Headers==============================
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Email: jl523136-at-gmail.com Name: Juntao Li
Organization: NY
Title-Subject: [Filtered] EXELFS analysis to obtain RDF
Question: Hi, everybody, I have a question about the EXELFS analysis. I try to use EXELFS analysis to obtain radial distribution function of amorphous materials. I followed the standard procedure, 1. Acquisition of high quality edge, deconvolution and background removal 2. Isolation of the oscillatory part of the intensity (normalization) 3. Scale conversion —(k) of into k space —(k) 4. Truncation of —(k) 5. Fourier distribution gives raw Radial Distribution Function (RDF) 6. Correction for phase shifts
} From step 4 to step 5 I was confused, I use Origin to the FFT, but in the image which I got, the X-axies is frequency, but in many papers I found that they got the RDF, where the x-axis is radial distance,the unit is angstrom or nm. I can not understand this, is there anybody can tell me how to get the RDF? Thanks a lot.
Although I have no direct experience to answer this question, on the basis of what is known about PFA fixation I would say there is a chance you lose a non-negligible part of your peptides. Do you retain enough to allow immunodetection is a question you can only answer by trying. A negative signal will be hard to interpret, though, because it could indeed come from this technical limitation. If I were you, I'd include as a control cells incubated with your protein at 4°C, this would allow the protein to bind its receptor but would block the internalization and subsequent processing (if you can block the processing after internalization it is even better, but you have to prove that the processing is actually blocked - with a gel for example). Your antibody should recognize the peptide sequence in the context of the full protein (?) so this would allow you to confirm that the immunodetection actually works.
Good luck,
Stephane
--- sshroff-at-bmrn.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the } Microscopy Listserver } using the WWW based Form at } --------------------------------------------------------------------------- } Remember this posting is most likely not from a } Subscriber, so when replying } please copy both sshroff-at-bmrn.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: sshroff-at-bmrn.com } Name: Shilpa } } Organization: BioMarin } } Title-Subject: [Filtered] peptide diffusion } } Question: Hi - } } I am looking at antigen presentation on the surface } of a cell line. I am first incubating the cell with } a protein (for about 2hrs). The cells are fixed } after the incubation in 4% paraformaldehyde, } permeabilized in TritonX. } } If my protein were fragmented, would I have to worry } about loosing the small peptides even though I fixed } in paraformaldehyde? I am staining the protien with } a fluorescent polyclonal to check its } internalization } } thanks } -shilpa } } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 9, 12 -- From zaluzec-at-microscopy.com Thu Jan 11 } 18:16:31 2007 } 9, 12 -- Received: from [206.69.208.22] } (mac22.zaluzec.com [206.69.208.22]) } 9, 12 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l0C0GUsX008394 } 9, 12 -- for {microscopy-at-microscopy.com} ; Thu, 11 } Jan 2007 18:16:31 -0600 } 9, 12 -- Mime-Version: 1.0 } 9, 12 -- X-Sender: (Unverified) } 9, 12 -- Message-Id: } {p06110402c1cc83ce9c1b-at-[206.69.208.22]} } 9, 12 -- Date: Thu, 11 Jan 2007 18:16:29 -0600 } 9, 12 -- To: microscopy-at-microscopy.com } 9, 12 -- From: sshroff-at-bmrn.com (by way of } MicroscopyListserver) } 9, 12 -- Subject: viaWWW: peptide diffusion } 9, 12 -- Content-Type: text/plain; } charset="us-ascii" } ==============================End of - } Headers============================== }
____________________________________________________________________________________ Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. http://answers.yahoo.com/dir/?link=list&sid=396545367
==============================Original Headers============================== 9, 21 -- From nizets2-at-yahoo.com Mon Jan 15 06:32:38 2007 9, 21 -- Received: from web37404.mail.mud.yahoo.com (web37404.mail.mud.yahoo.com [209.191.91.136]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0FCWbn4006357 9, 21 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jan 2007 06:32:37 -0600 9, 21 -- Received: (qmail 30535 invoked by uid 60001); 15 Jan 2007 12:32:37 -0000 9, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 21 -- s=s1024; d=yahoo.com; 9, 21 -- h=X-YMail-OSG:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 9, 21 -- b=1QY6nQMhnY626Zkh0FP5cmfWRlgq6SFa023kgy8Lp2m/0GYH6mx/X6I8z9QYh3Gc2jDf5jXwMrR6b3WvNfLqkSYbboPhADryPgBSlXz9OJS4DYIaGRUDKfifmSHx5fYNGCsUa3/ofQb+L1zDrz1aP6iGdLFvaRrU3Ghah4O7l14=; 9, 21 -- X-YMail-OSG: RhmN7WoVM1mmd.rM9qtcIRwllhN7nwPsG5qu.xGbhh2yvctn97Ttg_KaczWezN2myw-- 9, 21 -- Received: from [80.122.101.102] by web37404.mail.mud.yahoo.com via HTTP; Mon, 15 Jan 2007 04:32:36 PST 9, 21 -- Date: Mon, 15 Jan 2007 04:32:36 -0800 (PST) 9, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 9, 21 -- Subject: Re: [Microscopy] viaWWW: peptide diffusion 9, 21 -- To: sshroff-at-bmrn.com 9, 21 -- Cc: microscopy-at-microscopy.com 9, 21 -- In-Reply-To: {200701120024.l0C0OVwg019903-at-ns.microscopy.com} 9, 21 -- MIME-Version: 1.0 9, 21 -- Content-Type: text/plain; charset=iso-8859-1 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- Message-ID: {949223.29988.qm-at-web37404.mail.mud.yahoo.com} ==============================End of - Headers==============================
Atom Probe Engineers - 2 positions available Electron Microscope Unit at the University of Sydney, Australia
The Electron Microscope Unit (www.emu.usyd.edu.au) is located on the main campus of the University of Sydney and incorporates the Australian Key Centre for Microscopy and Microanalysis as its research and training arm. The Key Centre is a research leader in the field and has some of the most advanced facilities in the world for microscopy and microanalysis. As such, it seeks to develop techniques, expertise and understanding of the relationships between the structure and function of physical, chemical, biological and other systems. It also aims to provide leadership in the development of innovation and ingenuity in Australian science and engineering, and is seeking to recruit two outstanding Atom Probe Engineers to advance this aim.
The Atom Probe Engineers will provide the national user community with technical support for research using atom probe tomography, particularly as the Key Centre becomes the headquarters of a new National Microscopy and Microanalysis Research Facility during 2007. The Engineers will work closely with the Director and all unit staff to assure the success of the centre's research services and programs, and their integration with the new research facility, including national and international collaborations. They will provide technical support to users during their training and operation of the existing advanced atom probe and the forthcoming laser atom probe platform.
In this position, the Engineers will create solutions to specimen fabrication, determine sound running conditions for data acquisition, and conduct data reconstruction and analysis. So far as is possible, we prefer to train users in the relevant areas. In addition, there is scope for the appointees to undertake their own research in collaboration with the Director, and leading edge projects are available for immediate commencement. These exciting positions will require a commitment to technical excellence and will expose the appointees to cutting-edge research and development in nanotechnology. The advanced atom probe platforms are Imago LEAP(tm) instruments (www.imago.com).
The Engineers will have exceptional communication, interpersonal and problem solving skills, and the capacity to operate sophisticated scientific instrumentation. Skills in MS Office, email and internet will be essential, as will strong organisational and presentation skills and the ability to work both independently and in a team. In addition, the appointees will be committed to understanding and servicing the technical requirements of users, and be willing to undertake national and international travel.
To succeed, the appointees will have a Bachelor degree with Honours, and a background in applied physics, computer systems engineering, electronic engineering, mechanical/mechatronic engineering or materials engineering. A PhD in these areas will be highly advantageous.
These positions are full-time fixed term for five years, subject to the completion of a satisfactory probation period for new appointees. Following the initial five years, longer term appointments may be possible subject to available funding and facility demand. Membership of a University-approved superannuation scheme is a condition of employment for new appointees.
Remuneration package: AU$57,161- $64,050 p.a. (which includes a base salary Level 5 $48,302 - $54,123 p.a., leave loading and up to 17% employer's contribution to superannuation)
Depending on expertise, skills and qualifications, the remuneration package can be potentially higher than Level 5. This will be negotiated with quality candidates with relevant experience.
Closing: 2 February 2007
All applications must be completed online at the following url: http://positions.usyd.edu.au, search by position reference number 92079. Specific enquiries about the role can be directed to Kim Kiely on +61 2 9561 9068 or k.kiely-at-usyd.edu.au.
Kim Kiely Strategic Sourcing Specialist
E kim.kiely-at-hrx.com.au T 02 9561 9068 ABN 97 116 399 690 Level 6, 213 Miller Street, North Sydney, NSW 2060
==============================Original Headers============================== 15, 20 -- From kim.kiely-at-hrx.com.au Tue Jan 16 01:01:16 2007 15, 20 -- Received: from mail.hrx.com.au (210-193-133-5.macquarie.net.au [210.193.133.5] (may be forged)) 15, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0G71FtJ006159 15, 20 -- for {Microscopy-at-Microscopy.Com} ; Tue, 16 Jan 2007 01:01:16 -0600 15, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 15, 20 -- Content-class: urn:content-classes:message 15, 20 -- MIME-Version: 1.0 15, 20 -- Content-Type: text/plain; 15, 20 -- charset="us-ascii" 15, 20 -- Subject: Atom Probe Engineer at the University of Sydney 15, 20 -- Date: Tue, 16 Jan 2007 18:02:54 +1100 15, 20 -- Message-ID: {310E171DC233684F9BFBEBC32170AFFB7F7534-at-syd01ex0001.hrx.com.au} 15, 20 -- X-MS-Has-Attach: 15, 20 -- X-MS-TNEF-Correlator: 15, 20 -- Thread-Topic: Atom Probe Engineer at the University of Sydney 15, 20 -- Thread-Index: Acc5PFhFtzyt6o1VTvuaYkXKuJ5Bug== 15, 20 -- From: "Kim Kiely" {kim.kiely-at-hrx.com.au} 15, 20 -- To: {Microscopy-at-Microscopy.Com} 15, 20 -- Content-Transfer-Encoding: 8bit 15, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0G71FtJ006159 ==============================End of - Headers==============================
Research Associate - Nanostructural Analysis of Steels The Australian Key Centre for Microscopy and Microanalysis
The Australian Key Centre for Microscopy and Microanalysis (www.emu.usyd.edu.au) intends to appoint an outstanding Research Associate to conduct excellent research on a project to be conducted in collaboration with BlueScope Steel (www.bluescopesteel.com.au). The Research Associate will be based at the University of Sydney, and work closely with the staff at the BlueScope Steel Research Centre in Wollongong. The project contains scope for international travel in the form of visits to the United States for production trials and attendance at international conferences worldwide.
Castrip(r) is a revolutionary new method of producing steel strip directly from liquid steel that BlueScope Steel plan to commercialise in Australia. Currently, only plain carbon steels are produced using this method. This project will investigate microalloying additions known to be highly effective in conventional steels with an emphasis on nanoscale microstructure using advanced microscopy techniques including transmission electron microscopy (TEM) and atom probe tomography (APT). For the first time, we will explore and optimise the effect of these alloying additions in strip cast steels where dramatically higher cooling rates and special deoxidation practices present opportunities for innovative new design of steel nanostructure. Ultimately we will perform production trials of the most promising alloys with a view to developing design concepts for a new class of strip cast steels.
Located on the University's Camperdown campus, the Key Centre for Microscopy and Microanalysis headquarters the National Microscopy and Microanalysis Research Facility and is a major node of the ARC Centre of Excellence for Design in Light Metals. Researchers have access to an outstanding array of nanostructural analysis equipment, both within the Unit and at our partner nodes.
The successful applicant will be expected to engage in all aspects of the academic life of the facility. This includes approved collaboration with facility users, other facility staff and approved involvement with training activities. In this way, excellent opportunities for an academically-rich experience are available through this position. In addition, the appointee will be expected to write quality manuscripts that are suitable for publication in peer-reviewed journals.
To succeed, a recent PhD in metallurgy, materials science, engineering, physics, chemistry or a related discipline is essential. The position is full-time fixed term for 2 years, subject to the completion of a satisfactory probation period for new appointees. There is the possibility of further offers of employment for 12 months, subject to funding and need. Membership of a University approved superannuation scheme is a condition of employment for new appointees. The appointment can only commence after the Collaborating Organisation Agreement is signed with the BlueScope Steel.
Remuneration package: AU$72,327 - $77,638 p.a. (which includes a base salary Level A $61,117 - $65,605 p.a., leave loading and up to 17% employer's contribution to superannuation)
Closing: 2nd February 2007
All applications must be completed online at the following url: http://positions.usyd.edu.au and search by position reference number, 92070. Specific enquiries about the role can be directed to Kim Kiely on +61 2 9561 9068 or k.kiely-at-usyd.edu.au.
==============================Original Headers============================== 15, 20 -- From kim.kiely-at-hrx.com.au Tue Jan 16 01:03:08 2007 15, 20 -- Received: from mail.hrx.com.au (ns1.recruitasp.com.au [210.193.133.5]) 15, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0G7365f007628 15, 20 -- for {Microscopy-at-Microscopy.Com} ; Tue, 16 Jan 2007 01:03:07 -0600 15, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 15, 20 -- Content-class: urn:content-classes:message 15, 20 -- MIME-Version: 1.0 15, 20 -- Content-Type: text/plain; 15, 20 -- charset="us-ascii" 15, 20 -- Subject: Nanostructural Analysis of Steels 15, 20 -- Date: Tue, 16 Jan 2007 18:04:46 +1100 15, 20 -- Message-ID: {310E171DC233684F9BFBEBC32170AFFB7F7536-at-syd01ex0001.hrx.com.au} 15, 20 -- X-MS-Has-Attach: 15, 20 -- X-MS-TNEF-Correlator: 15, 20 -- Thread-Topic: Nanostructural Analysis of Steels 15, 20 -- Thread-Index: Acc5PJsRgXhhdXR+TAOBpeT6bUDfpQ== 15, 20 -- From: "Kim Kiely" {kim.kiely-at-hrx.com.au} 15, 20 -- To: {Microscopy-at-Microscopy.Com} 15, 20 -- Content-Transfer-Encoding: 8bit 15, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0G7365f007628 ==============================End of - Headers==============================
Research Associate - Advanced Microscopy The Australian Key Centre for Microscopy and Microanalysis
The Australian Key Centre for Microscopy and Microanalysis (www.emu.usyd.edu.au) currently has an outstanding opportunity available for a results-driven Research Associate to conduct specialised research investigating grain boundary chemistry using state-of-the-art microscopy and microanalysis techniques.
Located on the University's Camperdown campus, the Key Centre hosts world-class facilities, and is renowned for its research excellence. The Centre headquarters the National Microscopy and Microanalysis Research Facility and is a major node of the ARC Centre of Excellence for Design in Light Metals. Researchers have access to an outstanding array of nanostructural analysis equipment, both within the Unit and at our partner nodes.
The appointee will be involved in the study of the relationship between solute atmospheres at grain boundaries and the rate of grain boundary migration during annealing in practically important alloy systems. He or she will lead in the development of advanced experimental techniques utilising Focused Ion Beam (FIB) technology, Transmission Electron Microscopy (TEM) and Atom Probe Tomography (APT). This research project will be carried out in collaboration with Imago Scientific Instruments (www.imago.com), and contains scope for international travel in the form of research visits to the United States and attendance at international conferences worldwide.
The Key Centre attracts high-profile scientists and research groups, so an ability to communicate and work with others will be crucial. In addition, the appointee will need an ability to write quality manuscripts that are suitable for publication in peer-reviewed journals.
To succeed, a recent PhD in materials science, engineering, physics, chemistry or a related discipline is essential. This is the perfect opportunity for a recent graduate to conduct innovative research in a specialised field that attracts significant research coverage and funding.
The position is full-time fixed term for 2 years, subject to the completion of a satisfactory probation period for new appointees. There is the possibility of further offers of employment for 12 months, subject to funding and need. Membership of a University approved superannuation scheme is a condition of employment for new appointees.
Remuneration package: AU$72,327 - $77,638 p.a. (which includes a base salary Level A $61,117 - $65,605 p.a., leave loading and up to 17% employer's contribution to superannuation)
Closing: 2nd Febaury 2007
All applications must be completed online at the following url: http://positions.usyd.edu.au and search by position reference number, 92070. Specific enquiries about the role can be directed to Kim Kiely on +61 2 9561 9068 or k.kiely-at-usyd.edu.au.
==============================Original Headers============================== 12, 20 -- From kim.kiely-at-hrx.com.au Tue Jan 16 01:05:39 2007 12, 20 -- Received: from mail.hrx.com.au (ns1.recruitasp.com.au [210.193.133.5]) 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0G75bZP013668 12, 20 -- for {Microscopy-at-Microscopy.Com} ; Tue, 16 Jan 2007 01:05:38 -0600 12, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 20 -- Content-class: urn:content-classes:message 12, 20 -- MIME-Version: 1.0 12, 20 -- Content-Type: text/plain; 12, 20 -- charset="us-ascii" 12, 20 -- Subject: Research Associate in Advanced Microscopy 12, 20 -- Date: Tue, 16 Jan 2007 18:07:17 +1100 12, 20 -- Message-ID: {310E171DC233684F9BFBEBC32170AFFB7F7537-at-syd01ex0001.hrx.com.au} 12, 20 -- X-MS-Has-Attach: 12, 20 -- X-MS-TNEF-Correlator: 12, 20 -- Thread-Topic: Research Associate in Advanced Microscopy 12, 20 -- Thread-Index: Acc5PPUzjV1w16yVQWSc36zx9JRJmw== 12, 20 -- From: "Kim Kiely" {kim.kiely-at-hrx.com.au} 12, 20 -- To: {Microscopy-at-Microscopy.Com} 12, 20 -- Content-Transfer-Encoding: 8bit 12, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0G75bZP013668 ==============================End of - Headers==============================
Electron Microscope Unit (EMU) Careers in Microscopy and Microanalysis
Established in 1958, The University of Sydney's Electron Microscope Unit (EMU) produces quality research services, leading research programs, and internationally recognised research training in microscopy and microanalysis. It serves as national headquarters for the NANO Major National Research Facility (see: www.nano.org.au). The Unit recognises the substantial role that microscopy and microanalysis is set to play in the next decade, as biotechnology and nanoscience have increasing impact on science and society. The Unit has recently received significant competitive research grant funding and is undergoing major expansion. As a result, we are now seeking to recruit quality engineers and scientists with backgrounds in materials science and engineering, nanotechnology, physics, chemistry or other relevant engineering disciplines to undertake research in the Australian Key Centre for Microscopy & Microanalysis, the research and training arm of the EMU. We are presently mapping program and project requirements and have a number of positions to fill, including Postdoctoral Research Fellowships, Research Assistantships, Technical Staff Positions and PhD. Scholarships. A range of tenure in these positions is available, up to 5 years. We are interested to hear from recent graduates at the Bachelor or PhD. level that have a passion for research and are interested in investigating structure-property relationships in advanced materials using state-of-the-art microscopy and microanalysis approaches. A common theme in this research is to understand the structure and dynamics of materials structure towards the atomic level, and to relate to the engineering design and performance of materials. Necessarily, this will involve advances in microscopy research and techniques as well as advances in design in materials. This research builds on national and international collaborations, so we are looking for researchers that are also willing to undertake travel and short secondments in the laboratories of our colleagues.
The projects relate to design in:
* Light Alloys (alloys of Mg, Ti and Al) * Novel Steels Systems * Spintronic Systems * Other Nanomaterials
Demonstrated research potential is essential Research experience in relevant fields will be required in some of these positions. For more details on possible projects, see www.emu.usyd.edu.au and follow the "career" link. For more details on selection criteria, see the attachment "EOI - Generic Selection Criteria" below.
To register interest for one of these positions, follow the link http://positions.usyd.edu.au and search by position reference number 92067. For enquiries about these roles please call Fabrice Noël on +61 2 9036 7295 or f.noel-at-usyd.edu.au. Closing: Interested parties are urged to express interest as soon as possible to feed into the research mapping process, certainly by no later than Friday, 2 February, 2007.
==============================Original Headers============================== 12, 20 -- From kim.kiely-at-hrx.com.au Tue Jan 16 01:08:15 2007 12, 20 -- Received: from mail.hrx.com.au (210-193-133-5.macquarie.net.au [210.193.133.5] (may be forged)) 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0G78DPC022723 12, 20 -- for {Microscopy-at-Microscopy.Com} ; Tue, 16 Jan 2007 01:08:14 -0600 12, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 20 -- Content-class: urn:content-classes:message 12, 20 -- MIME-Version: 1.0 12, 20 -- Content-Type: text/plain; 12, 20 -- charset="iso-8859-1" 12, 20 -- Subject: Microscopy and Microanalysis at the University of Sydney 12, 20 -- Date: Tue, 16 Jan 2007 18:09:53 +1100 12, 20 -- Message-ID: {310E171DC233684F9BFBEBC32170AFFB7F7538-at-syd01ex0001.hrx.com.au} 12, 20 -- X-MS-Has-Attach: 12, 20 -- X-MS-TNEF-Correlator: 12, 20 -- Thread-Topic: Microscopy and Microanalysis at the University of Sydney 12, 20 -- Thread-Index: Acc5PVIfM3GFH90GSwyP4NvVLCUdHw== 12, 20 -- From: "Kim Kiely" {kim.kiely-at-hrx.com.au} 12, 20 -- To: {Microscopy-at-Microscopy.Com} 12, 20 -- Content-Transfer-Encoding: 8bit 12, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0G78DPC022723 ==============================End of - Headers==============================
I have a request for experts in SEM who have some experience with eukaryotic cells. We have a basic SEM which is normally used to study minerals, so we are not equipped with critical point dryers and the like. Now I would like to have a look at some cells in culture with the SEM but I have no idea how I could prepare the cells without critical point dryer. Of course if I simply fix and dehydrate in acetone, then air dry the cells I expect the structure to crumble. Well perhaps it won't give nice pictures but perhaps it will be enough for what I want to see. On the other hand perhaps I could keep them in a semi-hydrated state and look at them at 10 Pa vaccum (whatever "semi-hydrated state means ;-)).
I would be grateful if you cared to share your opinion on this subjet with me.
Stephane
____________________________________________________________________________________ Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. http://games.yahoo.com/games/front
==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Tue Jan 16 03:14:48 2007 6, 19 -- Received: from web37411.mail.mud.yahoo.com (web37411.mail.mud.yahoo.com [209.191.91.143]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0G9Emoo019552 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jan 2007 03:14:48 -0600 6, 19 -- Received: (qmail 62303 invoked by uid 60001); 16 Jan 2007 09:14:47 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 6, 19 -- b=kpkcnoBDUHMcVkk3aIfi3ch5Ffk324ZCkbIAnLfSxMO5yzLGCpa74RgxqXcpb6rCkUqNh6CevqxvS783hV8rc5yRiXjGPlhG+sMnVSwX/qheKVKYH8tg8dOuIcXQ0xvXvOS/v0G8SSlqv8teAEkzdXQKlgJxBBldpWTS2jBvJxk=; 6, 19 -- X-YMail-OSG: 6E_tvvQVM1lhn5yjbcRFZ33VO8xzQy.Y0.qekDzkGSSd9Vs3SDF0WHtJsnif0Jao410DY2csNk2MjHuUwU1.68o6dDetO9BirDlwIulsuZg6gJF0FQgVpzzHlnJ9St8l8kxEzjvOlJUvb3w- 6, 19 -- Received: from [80.122.101.102] by web37411.mail.mud.yahoo.com via HTTP; Tue, 16 Jan 2007 01:14:47 PST 6, 19 -- Date: Tue, 16 Jan 2007 01:14:47 -0800 (PST) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 -- Subject: preparation of cells for SEM - question 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- Message-ID: {636541.62156.qm-at-web37411.mail.mud.yahoo.com} ==============================End of - Headers==============================
Stephane, I have always found your posts interesting so it is nice to be able to help you. I think critical point drying is best for cell preparation but we tend to use a hexamethyldisilazane (HMDS) dehydration protocol as it is much easier.
I think you will find looking at wet cells problematic. With a tungsten gun XL30 ESEM I find it very difficult to get adequate resolution with cultured cells.
My HMDS protocol is given below. If anyone would like to suggest improvements please fire away. Although fixing in PBS goes against EM principals the results for modest magnification SEM are often good.
Dave
Preparation of dry samples for SEM using HMDS
Safety Work in fume hood and use gloves Retain ethanol and HMDS (hexamethyldisilazane) for disposal in non-chlorinated waste bottle
Fixation
Fix in 4% glutaraldehyde in buffer (usually 0.1M )* Leave for 1 hr at room temperature or 24hrs in the fridge Rinse in buffer x3 (total storage time should exceed fixation time by a factor of 3).
SEM Dehydration
5m in 20% ethanol 5m in 30% ethanol 5m in 50% ethanol 5m in 70% ethanol 5m in 90% ethanol 5m in 100% ethanol 5m in 100% ethanol
5m in 100% ethanol / HMDS (2:1) 5m in 100% ethanol / HMDS (2:2) 5m in 100% ethanol / HMDS (1:2) 5m in 100% HMDS 5m in 100% HMDS 5m in 100% HMDS
Remove specimen from HMDS and place on filter paper in a petri dish and leave in fume hood until dry.
*For 4% glutaraldehyde in 0.1M buffer Take, say, 50mls of 0.2M buffer, 34ml d water and 16ml of 25% glutaraldehyde.
0.1M Phosphate buffer or sodium cacodylate are used.
Fixation Using PBS 8.4ml PBS 1.6ml glutaraldehyde
Gives 10ml of a 4% glutaraldehyde fixative
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: 16 January 2007 09:20 To: David Patton
Dear listers,
I have a request for experts in SEM who have some experience with eukaryotic cells. We have a basic SEM which is normally used to study minerals, so we are not equipped with critical point dryers and the like. Now I would like to have a look at some cells in culture with the SEM but I have no idea how I could prepare the cells without critical point dryer. Of course if I simply fix and dehydrate in acetone, then air dry the cells I expect the structure to crumble. Well perhaps it won't give nice pictures but perhaps it will be enough for what I want to see. On the other hand perhaps I could keep them in a semi-hydrated state and look at them at 10 Pa vaccum (whatever "semi-hydrated state means ;-)).
I would be grateful if you cared to share your opinion on this subjet with me.
Stephane
________________________________________________________________________ ____________ Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. http://games.yahoo.com/games/front
==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Tue Jan 16 03:14:48 2007 6, 19 -- Received: from web37411.mail.mud.yahoo.com (web37411.mail.mud.yahoo.com [209.191.91.143]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0G9Emoo019552 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jan 2007 03:14:48 -0600 6, 19 -- Received: (qmail 62303 invoked by uid 60001); 16 Jan 2007 09:14:47 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Co ntent-Transfer-Encoding:Message-ID; 6, 19 -- b=kpkcnoBDUHMcVkk3aIfi3ch5Ffk324ZCkbIAnLfSxMO5yzLGCpa74RgxqXcpb6rCkUqNh6 CevqxvS783hV8rc5yRiXjGPlhG+sMnVSwX/qheKVKYH8tg8dOuIcXQ0xvXvOS/v0G8SSlqv8 teAEkzdXQKlgJxBBldpWTS2jBvJxk=; 6, 19 -- X-YMail-OSG: 6E_tvvQVM1lhn5yjbcRFZ33VO8xzQy.Y0.qekDzkGSSd9Vs3SDF0WHtJsnif0Jao410DY2cs Nk2MjHuUwU1.68o6dDetO9BirDlwIulsuZg6gJF0FQgVpzzHlnJ9St8l8kxEzjvOlJUvb3w- 6, 19 -- Received: from [80.122.101.102] by web37411.mail.mud.yahoo.com via HTTP; Tue, 16 Jan 2007 01:14:47 PST 6, 19 -- Date: Tue, 16 Jan 2007 01:14:47 -0800 (PST) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 -- Subject: preparation of cells for SEM - question 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- Message-ID: {636541.62156.qm-at-web37411.mail.mud.yahoo.com} ==============================End of - Headers==============================
This incoming email to UWE has been independently scanned for viruses by McAfee anti-virus software and none were detected
This email was independently scanned for viruses by McAfee anti-virus software and none were found
==============================Original Headers============================== 38, 34 -- From David.Patton-at-uwe.ac.uk Tue Jan 16 03:36:06 2007 38, 34 -- Received: from mailapp03.uwe.ac.uk (mailapp03.uwe.ac.uk [164.11.132.65]) 38, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0G9a5Gk030756 38, 34 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jan 2007 03:36:05 -0600 38, 34 -- Received: from (mta02.uwe.ac.uk [164.11.132.62]) by mailapp03.uwe.ac.uk with smtp 38, 34 -- id 2d0f_31ed9552_a544_11db_97a3_00142221cca9; 38, 34 -- Tue, 16 Jan 2007 09:30:35 +0000 38, 34 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 38, 34 -- (egen-uwe01.uwe.ac.uk [164.11.249.121]) 38, 34 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 38, 34 -- 2005)) with ESMTP id {0JBY00AR2FZU3S-at-mta02.uwe.ac.uk} for 38, 34 -- microscopy-at-microscopy.com; Tue, 16 Jan 2007 09:35:54 +0000 (GMT) 38, 34 -- Date: Tue, 16 Jan 2007 09:35:06 +0000 38, 34 -- From: David Patton {David.Patton-at-uwe.ac.uk} 38, 34 -- Subject: RE: [Microscopy] preparation of cells for SEM - question 38, 34 -- In-reply-to: {200701160920.l0G9KGNq026487-at-ns.microscopy.com} 38, 34 -- To: nizets2-at-yahoo.com 38, 34 -- Cc: microscopy-at-microscopy.com 38, 34 -- Message-id: {F247F674896BE243AD8263C5280E2BDB02B0AECD-at-egen-uwe01} 38, 34 -- MIME-version: 1.0 38, 34 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 38, 34 -- Content-type: text/plain; charset=us-ascii 38, 34 -- Content-class: urn:content-classes:message 38, 34 -- Thread-topic: [Microscopy] preparation of cells for SEM - question 38, 34 -- Thread-index: Acc5T4/BzWF0tJWbTBOyK0ths3Tq+wAAIe4Q 38, 34 -- X-MS-Has-Attach: 38, 34 -- X-MS-TNEF-Correlator: 38, 34 -- X-NAIMIME-Disclaimer: 1 38, 34 -- X-NAIMIME-Modified: 1 38, 34 -- X-NAI-Spam-Score: -0.3 38, 34 -- X-NAI-Spam-Rules: 1 Rules triggered 38, 34 -- BAYES_30=-0.3 38, 34 -- Content-Transfer-Encoding: 8bit 38, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0G9a5Gk030756 ==============================End of - Headers==============================
If your cells are in suspension I would coat cover slips with poly-l-lysine (1mg/ml in distilled water), place a drop of the suspension onto the cover slips and allow to settle for up to an hour in a humidity chamber. Then, after the cells attach, just gently rinse the cover slips, fix, and dehydrate using David's HMDS protocol.
I would suggest 2% glutaraldehyde in buffer (0.1M phosphate, cacodylate, or common buffer for biological EM) for 10-15 minutes, followed by three or more buffer rinses, post-fixation in 1% osmium tetroxide (buffered or aqueous) for the same time, followed by water rinses for 5-10 minutes each, then into your dehydration schedule. Sputter coat and mount.
Good luck.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, January 16, 2007 3:16 AM To: Tindall, Randy D.
Dear listers,
I have a request for experts in SEM who have some experience with eukaryotic cells. We have a basic SEM which is normally used to study minerals, so we are not equipped with critical point dryers and the like. Now I would like to have a look at some cells in culture with the SEM but I have no idea how I could prepare the cells without critical point dryer. Of course if I simply fix and dehydrate in acetone, then air dry the cells I expect the structure to crumble. Well perhaps it won't give nice pictures but perhaps it will be enough for what I want to see. On the other hand perhaps I could keep them in a semi-hydrated state and look at them at 10 Pa vaccum (whatever "semi-hydrated state means ;-)).
I would be grateful if you cared to share your opinion on this subjet with me.
Stephane
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==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Tue Jan 16 03:14:48 2007 6, 19 -- Received: from web37411.mail.mud.yahoo.com (web37411.mail.mud.yahoo.com [209.191.91.143]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0G9Emoo019552 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jan 2007 03:14:48 -0600 6, 19 -- Received: (qmail 62303 invoked by uid 60001); 16 Jan 2007 09:14:47 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Co ntent-Transfer-Encoding:Message-ID; 6, 19 -- b=kpkcnoBDUHMcVkk3aIfi3ch5Ffk324ZCkbIAnLfSxMO5yzLGCpa74RgxqXcpb6rCkUqNh6 CevqxvS783hV8rc5yRiXjGPlhG+sMnVSwX/qheKVKYH8tg8dOuIcXQ0xvXvOS/v0G8SSlqv8 teAEkzdXQKlgJxBBldpWTS2jBvJxk=; 6, 19 -- X-YMail-OSG: 6E_tvvQVM1lhn5yjbcRFZ33VO8xzQy.Y0.qekDzkGSSd9Vs3SDF0WHtJsnif0Jao410DY2cs Nk2MjHuUwU1.68o6dDetO9BirDlwIulsuZg6gJF0FQgVpzzHlnJ9St8l8kxEzjvOlJUvb3w- 6, 19 -- Received: from [80.122.101.102] by web37411.mail.mud.yahoo.com via HTTP; Tue, 16 Jan 2007 01:14:47 PST 6, 19 -- Date: Tue, 16 Jan 2007 01:14:47 -0800 (PST) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 -- Subject: preparation of cells for SEM - question 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- Message-ID: {636541.62156.qm-at-web37411.mail.mud.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 20, 26 -- From TindallR-at-missouri.edu Tue Jan 16 12:22:32 2007 20, 26 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 20, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0GIMWsj023906 20, 26 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jan 2007 12:22:32 -0600 20, 26 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 20, 26 -- Tue, 16 Jan 2007 12:22:31 -0600 20, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 20, 26 -- Content-class: urn:content-classes:message 20, 26 -- MIME-Version: 1.0 20, 26 -- Content-Type: text/plain; 20, 26 -- charset="us-ascii" 20, 26 -- Subject: RE: [Microscopy] preparation of cells for SEM - question 20, 26 -- Date: Tue, 16 Jan 2007 12:22:31 -0600 20, 26 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68CE3-at-UM-XMAIL08.um.umsystem.edu} 20, 26 -- in-reply-to: {200701160916.l0G9GKG9021537-at-ns.microscopy.com} 20, 26 -- X-MS-Has-Attach: 20, 26 -- X-MS-TNEF-Correlator: 20, 26 -- Thread-Topic: [Microscopy] preparation of cells for SEM - question 20, 26 -- Thread-Index: Acc5Tv0//VNi6xXfS1e9n6fo+SQCaAAScKjQ 20, 26 -- References: {200701160916.l0G9GKG9021537-at-ns.microscopy.com} 20, 26 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 20, 26 -- To: {nizets2-at-yahoo.com} 20, 26 -- Cc: {microscopy-at-microscopy.com} 20, 26 -- X-OriginalArrivalTime: 16 Jan 2007 18:22:31.0760 (UTC) FILETIME=[4967C500:01C7399B] 20, 26 -- Content-Transfer-Encoding: 8bit 20, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0GIMWsj023906 ==============================End of - Headers==============================
On Jan 16, 2007, at 1:14 AM, nizets2-at-yahoo.com wrote:
} I have a request for experts in SEM who have some } experience with eukaryotic cells. } We have a basic SEM which is normally used to study } minerals, so we are not equipped with critical point } dryers and the like. Now I would like to have a look } at some cells in culture with the SEM but I have no } idea how I could prepare the cells without critical } point dryer. Of course if I simply fix and dehydrate } in acetone, then air dry the cells I expect the } structure to crumble. Well perhaps it won't give nice } pictures but perhaps it will be enough for what I want } to see. On the other hand perhaps I could keep them in } a semi-hydrated state and look at them at 10 Pa vaccum } (whatever "semi-hydrated state means ;-)). } } I would be grateful if you cared to share your opinion } on this subjet with me. } Dear Stephane, When I was at the high-voltage TEM, I designed a hydration stage, which allowed me to examine cells that were fully hydrated--as determined by the fact that upon removal from the scope, at least some of the cells were still viable. These were prepared by placing formvar/carbon coated gold grids in the culture dish, so the cells would grow on the grids, then just removing the grids from the dish, placing them in a 100% humidity chamber, blotting off the excess fluid, and transferring the grids to the hydration stage maintaining the humidity as close to 100% as possible. If you can operate your VPSEM at the vapor pressure of water for the temperature of the specimen chamber (~25 torr), then you can look at them fully hydrated. I do not know, however, whether you will see anything of interest, or just the surface of the thin film of water that will still coat the cells. Good luck. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Tue Jan 16 18:33:28 2007 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0H0XRtM009854 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 16 Jan 2007 18:33:28 -0600 4, 22 -- Received: from water-dog.caltech.edu (water-dog [192.168.1.26]) 4, 22 -- by earth-ox-postvirus (Postfix) with ESMTP id 386C510A4AD 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 16 Jan 2007 16:15:49 -0800 (PST) 4, 22 -- Received: from [192.168.159.233] (jpix-01.caltech.edu [131.215.2.133]) 4, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id C24DE35B20 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 16 Jan 2007 15:55:41 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v624) 4, 22 -- In-Reply-To: {200701160914.l0G9EvlZ019715-at-ns.microscopy.com} 4, 22 -- References: {200701160914.l0G9EvlZ019715-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {10f5fb65833744fd4afb91860f232bc1-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] preparation of cells for SEM - question 4, 22 -- Date: Tue, 16 Jan 2007 16:03:20 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.624) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.3.3 ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (chappell.mark-at-epa.gov) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, January 16, 2007 at 13:59:24 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both chappell.mark-at-epa.gov as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: chappell.mark-at-epa.gov Name: Mark Chappell
Organization: U.S. EPA
Education: Graduate College
Location: Cincinnati, OH
Title: SEM/EDX of bird feather
Question: I want to map the Hg in a contaminated bird feather using SEM/EDX/WDS. What's the best way to prepare the sample to minimize charging?
First look at Hg Z=80. So, high Z. M alpha=2.191KeV. L alpha=9.986KeV.
So, for a good results you can do 5KV and look for the Ma or do 20KV and look for Ma and La.
Coat the specimen with Pd, Ir, C, but not Au (La=9.710KeV). Doing as suggested will or should put Hg by itself as a EDS peak.
gary g.
At 06:02 PM 1/16/2007, you wrote:
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==============================Original Headers============================== 13, 20 -- From gary-at-gaugler.com Tue Jan 16 20:22:49 2007 13, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0H2Mmjm000978 13, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jan 2007 20:22:48 -0600 13, 20 -- Message-Id: {200701170222.l0H2Mmjm000978-at-ns.microscopy.com} 13, 20 -- Received: (qmail 15696 invoked from network); 16 Jan 2007 18:22:39 -0800 13, 20 -- Received: by simscan 1.1.0 ppid: 15678, pid: 15693, t: 0.1742s 13, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 13, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 13, 20 -- by qsmtp1 with SMTP; 16 Jan 2007 18:22:39 -0800 13, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 20 -- Date: Tue, 16 Jan 2007 18:24:43 -0800 13, 20 -- To: chappell.mark-at-epa.gov 13, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 13, 20 -- Subject: Re: [Microscopy] AskAMicroscopist: SEM/EDX of bird feather 13, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 13, 20 -- In-Reply-To: {200701170202.l0H22ZtI025116-at-ns.microscopy.com} 13, 20 -- References: {200701170202.l0H22ZtI025116-at-ns.microscopy.com} 13, 20 -- Mime-Version: 1.0 13, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-57F5541 ==============================End of - Headers==============================
As I think about this....this may not be possible.
The amount of Hg in the feathers are probably PPM. EDS will not show this. You would need WDS or FTIR to show up the small amounts....unless the feathers are really loaded with Hg. The shape of the feathers will also affect collection due to difflection of the x-rays. But you should still get decent results to show if any TRACE amounts of Hg are present.
gary g.
At 06:02 PM 1/16/2007, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Tue Jan 16 20:27:47 2007 10, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0H2RkcY011922 10, 20 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jan 2007 20:27:46 -0600 10, 20 -- Message-Id: {200701170227.l0H2RkcY011922-at-ns.microscopy.com} 10, 20 -- Received: (qmail 1348 invoked from network); 16 Jan 2007 18:22:21 -0800 10, 20 -- Received: by simscan 1.1.0 ppid: 1336, pid: 1346, t: 0.1706s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp4 with SMTP; 16 Jan 2007 18:22:21 -0800 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Tue, 16 Jan 2007 18:29:42 -0800 10, 20 -- To: chappell.mark-at-epa.gov 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] AskAMicroscopist: SEM/EDX of bird feather 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200701170202.l0H22ZtI025116-at-ns.microscopy.com} 10, 20 -- References: {200701170202.l0H22ZtI025116-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-57F5541 ==============================End of - Headers==============================
I am looking for the information on BALTEK - RES-010 Benchtop Rapid Etching System. If you have any experience with the ion milling such as artifact and over milling, please provide the information.
We have an old ion milling from BALTEK, but I heard that it always overmills. Now our instrument has some problems, but no one wants to fix it because of over milling problem. I looked at the instrument and I found that the gun is much similar to the gun from Technoorg-Linda IV3. Based on some experience with IV3, I think that people at our university might not use proper way to operate it.
If you use BALTEK - RES-010, I would like to know the milling conditions such as angle, voltage, and current. Also, I would like to know if you have any experience of serious artifacts such as reaction, decomposition or phase transformation. Thank you,
Hiromi Konishi, Ph.D. UW-Madison
==============================Original Headers============================== 6, 30 -- From hikonishi-at-gmail.com Tue Jan 16 23:20:03 2007 6, 30 -- Received: from py-out-1112.google.com (py-out-1112.google.com [64.233.166.178]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0H5K2dO027924 6, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 16 Jan 2007 23:20:03 -0600 6, 30 -- Received: by py-out-1112.google.com with SMTP id b50so1226286pyh 6, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 16 Jan 2007 21:20:00 -0800 (PST) 6, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 30 -- d=gmail.com; s=beta; 6, 30 -- h=received:message-id:from:to:subject:date:mime-version:content-type:content-transfer-encoding:x-priority:x-msmail-priority:x-mailer:x-mimeole; 6, 30 -- b=IaBQGRKwpfKAkRx8L8wGRHk3RnDqEJpDCCZNNKQSAZdIlxSXe9NMlY+KDGtAFfFdFcRKMHSloGXp0vQZeSKX66l0oB81xuJkUkNI9T+GJnlg+eWHE+xuejwjiX/j/yHPAzTSu/qcbAw7NztRXONuqzPOQItfD5YjXCZx5JNNoNk= 6, 30 -- Received: by 10.35.103.12 with SMTP id f12mr11756749pym.1169011200239; 6, 30 -- Tue, 16 Jan 2007 21:20:00 -0800 (PST) 6, 30 -- Received: from HKONISHI ( [216.165.154.16]) 6, 30 -- by mx.google.com with ESMTP id f57sm9087298pyh.2007.01.16.21.19.59; 6, 30 -- Tue, 16 Jan 2007 21:19:59 -0800 (PST) 6, 30 -- Message-ID: {000901c739f7$2d245690$0200a8c0-at-HKONISHI} 6, 30 -- From: "Hiromi Konishi" {hikonishi-at-gmail.com} 6, 30 -- To: {Microscopy-at-microscopy.com} 6, 30 -- Subject: BALTEK - RES-010 Benchtop Rapid Etching System 6, 30 -- Date: Tue, 16 Jan 2007 23:20:10 -0600 6, 30 -- MIME-Version: 1.0 6, 30 -- Content-Type: text/plain; 6, 30 -- format=flowed; 6, 30 -- charset="iso-2022-jp"; 6, 30 -- reply-type=original 6, 30 -- Content-Transfer-Encoding: 7bit 6, 30 -- X-Priority: 3 6, 30 -- X-MSMail-Priority: Normal 6, 30 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 6, 30 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
} } } Question: I want to map the Hg in a contaminated bird feather using } SEM/EDX/WDS. What's the best way to prepare the sample to minimize } charging?
When you say "contaminated" do you mean massive contamination, millions of times greater than what a human would get in their hair (thru metabolism) eating fish that had "high" levels from Hg used in adjacent gold mining/extraction? Only in that case would an electron-based microanalytical technique be of any use.
I attempted to measure Hg in hair from native people from Brazil who lived near gold extraction areas.The idea was to see if we could see variations in the hair along length (thus, versus time). Using WDS EPMA. I knew the approximate levels (thru some technique like INAA or ICPMS or XRF, I forget) and it was high ppb or low ppm (again, I forget the details). But I could never put enough current (I probably went up to 10 or 20 nA) to see anything.
John
==============================Original Headers============================== 5, 25 -- From johnf-at-geology.wisc.edu Wed Jan 17 07:12:42 2007 5, 25 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0HDCfcB014198 5, 25 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jan 2007 07:12:42 -0600 5, 25 -- Received: from localhost (localhost [127.0.0.1]) 5, 25 -- by localhost (Postfix) with ESMTP id 134F720D31; 5, 25 -- Wed, 17 Jan 2007 07:12:40 -0600 (CST) 5, 25 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) 5, 25 -- by localhost (geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 5, 25 -- with ESMTP id 27330-08; Wed, 17 Jan 2007 07:12:34 -0600 (CST) 5, 25 -- Received: from [192.168.1.103] (24-183-52-127.dhcp.mdsn.wi.charter.com [24.183.52.127]) 5, 25 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 5, 25 -- (No client certificate requested) 5, 25 -- by ice.geology.wisc.edu (Postfix) with ESMTP id 5B2A320D08; 5, 25 -- Wed, 17 Jan 2007 07:12:34 -0600 (CST) 5, 25 -- Mime-Version: 1.0 5, 25 -- Message-Id: {p06210201c1d3cfb242f0-at-[192.168.0.122]} 5, 25 -- In-Reply-To: {200701170205.l0H2595J030194-at-ns.microscopy.com} 5, 25 -- References: {200701170205.l0H2595J030194-at-ns.microscopy.com} 5, 25 -- Date: Wed, 17 Jan 2007 07:14:58 -0600 5, 25 -- To: chappell.mark-at-epa.gov, microscopy-at-microscopy.com 5, 25 -- From: John Fournelle {johnf-at-geology.wisc.edu} 5, 25 -- Subject: Re: SEM/EDX of bird feather 5, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 25 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu ==============================End of - Headers==============================
Wow, Some really different answers. I seem to remember Hg (2.19) falls close to S (2.30), so I would use 25 kv because I would want to see both the 2.19 M line and 9.8 L line. I like to be able to see at least two lines, when possible, for any element. I would skip coating, having tried it and always unhappy with my results and go with low pressure.
I suspect the comments about detectable level as are on target, but you could very well surprise us.
Please let me know how this works out as I played with feathers using light and SEM and have an interest.
chappell.mark-at-epa .gov To: frank.karl-at-degussa.com cc: 01/16/2007 09:02 Subject: [Microscopy] AskAMicroscopist: SEM/EDX of bird feather PM Please respond to chappell.mark
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This Question was submitted to Ask-A-Microscopist by (chappell.mark-at-epa.gov) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, January 16, 2007 at 13:59:24 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both chappell.mark-at-epa.gov as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: chappell.mark-at-epa.gov Name: Mark Chappell
Organization: U.S. EPA
Education: Graduate College
Location: Cincinnati, OH
Title: SEM/EDX of bird feather
Question: I want to map the Hg in a contaminated bird feather using SEM/EDX/WDS. What's the best way to prepare the sample to minimize charging?
I apologise for taking space for this request, but I have looked many places without success. Perhaps someone here may be able to give me some pointers.
I'm looking for a keyboard for a SEM: a Zeiss DSM 9XX A. It could be from a 940, 950, or 960 as I believe these are all the same keyboards. This is for a SEM that is being used in my local high school so I'm looking for a used, functioning keyboard that is not expensive.
If anyone knows where I might find this, please contact me. As I imagine this is of no interest to the group, please respond to me directly.
I'm located in Cold Spring, New York USA.
Thank you,
dj
==============================Original Headers============================== 7, 16 -- From dljones-at-bestweb.net Wed Jan 17 08:01:51 2007 7, 16 -- Received: from vms044pub.verizon.net (vms044pub.verizon.net [206.46.252.44]) 7, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0HE1pkq004628 7, 16 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Jan 2007 08:01:51 -0600 7, 16 -- Received: from localhost ([71.247.123.130]) 7, 16 -- by vms044.mailsrvcs.net (Sun Java System Messaging Server 6.2-6.01 (built Apr 7, 16 -- 3 2006)) with ESMTPA id {0JC000NCFMYU4B30-at-vms044.mailsrvcs.net} for 7, 16 -- Microscopy-at-microscopy.com; Wed, 17 Jan 2007 08:01:47 -0600 (CST) 7, 16 -- Date: Wed, 17 Jan 2007 09:00:49 -0500 (Eastern Standard Time) 7, 16 -- From: "David L. Jones" {dljones-at-bestweb.net} 7, 16 -- Subject: looking for parts 7, 16 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 7, 16 -- To: Microscopy-at-microscopy.com 7, 16 -- Message-id: {Pine.WNT.4.64.0701170841590.2632-at-H-F1} 7, 16 -- MIME-version: 1.0 7, 16 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
While our main sputter coater is out for a much-needed overhaul, we are relying on a backup coater, namely a Polaron E5100. The unit is operating beautifully, except that the voltage dial has worked loose at some point and I can no longer tell where the zero point, and thus the proper operating voltage, is located on the scale. This is because the scale starts at 2.0, not zero, and I have no reference to line up the indicator with. I tried looking at the set-screw scar on the knob shaft, but it didn't help. Am I missing something obvious? (Wouldn't be the first time.....)
f you will go to http://www.emc.missouri.edu/lookatthis.htm to see the images of this coater hopefully this will make sense.
Can someone with a similar unit kindly let me know about where the zero point would be on this machine? I can get it to coat by trial and error, but it would be nice to have a baseline to experiment from.
Many thanks!
Searching for zero, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 9, 23 -- From TindallR-at-missouri.edu Wed Jan 17 09:39:47 2007 9, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0HFdlGO018946 9, 23 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jan 2007 09:39:47 -0600 9, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 9, 23 -- Wed, 17 Jan 2007 09:39:47 -0600 9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 23 -- Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="us-ascii" 9, 23 -- Subject: Polaron E5100 sputter coater question 9, 23 -- Date: Wed, 17 Jan 2007 09:39:46 -0600 9, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68CF1-at-UM-XMAIL08.um.umsystem.edu} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: Polaron E5100 sputter coater question 9, 23 -- Thread-Index: Acc6TbbzCo8D3MqPS+a3y10wR2ngWQ== 9, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 9, 23 -- To: {microscopy-at-microscopy.com} 9, 23 -- X-OriginalArrivalTime: 17 Jan 2007 15:39:47.0209 (UTC) FILETIME=[B7B14790:01C73A4D] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0HFdlGO018946 ==============================End of - Headers==============================
There may be a better way to do this- nondestructively- using x-rays from the Advanced Photon Source Users' Facility at Argonne National Laboratory. See this analysis of lead in human hair (a rather famous, human, at that):
If you are interested, I can put you in contact with the investigators on this project.
Jeffrey A. Fortner, Ph.D. Chemical Engineering Division Argonne National Laboratory Argonne, IL 60439 fortner(at)cmt.anl.gov
. -----Original Message----- X-from: chappell.mark-at-epa.gov [mailto:chappell.mark-at-epa.gov] Sent: Tuesday, January 16, 2007 8:02 PM To: Fortner, Jeffrey A.
This Question was submitted to Ask-A-Microscopist by (chappell.mark-at-epa.gov) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, January 16, 2007 at 13:59:24 Remember to consider the Grade/Age of the student when considering the Question ------------------------------------------------------------------------ --- Please reply to both chappell.mark-at-epa.gov as well as to the Microscopy Listserver ------------------------------------------------------------------------ ---
Email: chappell.mark-at-epa.gov Name: Mark Chappell
Organization: U.S. EPA
Education: Graduate College
Location: Cincinnati, OH
Title: SEM/EDX of bird feather
Question: I want to map the Hg in a contaminated bird feather using SEM/EDX/WDS. What's the best way to prepare the sample to minimize charging?
Work on elemental composition in hair was done back in the 70s by Dr. Walter McCrone using neutron activation. This was used to map elements by concentration along the shaft. The basis was to look at hair differentiation in forensics.
I recall Walter mentioning it in a short presentation at Inter-Micro ca. 2000? as well as in a conversation I had with him. I believe he also published a piece in the The Microscope.
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
This message is from pH2. This message and any attachments may contain legally privileged or confidential information, and are intended only for the individual or entity identified above as the addressee. If you are not the addressee, or if this message has been addressed to you in error, you are not authorized to read, copy, or distribute this message and any attachments, and we ask that you please delete this message and attachments (including all copies) and notify the sender by return e-mail or by phone at 317-718-7020. Delivery of this message and any attachments to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or a privilege. All personal messages express views only of the sender, which are not to be attributed to pH2 and may not be copied or distributed without this statement.
-----Original Message----- X-from: johnf-at-geology.wisc.edu [mailto:johnf-at-geology.wisc.edu] Sent: Wednesday, January 17, 2007 8:22 AM To: ph2-at-sprynet.com
} } } Question: I want to map the Hg in a contaminated bird feather using } SEM/EDX/WDS. What's the best way to prepare the sample to minimize } charging?
When you say "contaminated" do you mean massive contamination, millions of times greater than what a human would get in their hair (thru metabolism) eating fish that had "high" levels from Hg used in adjacent gold mining/extraction? Only in that case would an electron-based microanalytical technique be of any use.
I attempted to measure Hg in hair from native people from Brazil who lived near gold extraction areas.The idea was to see if we could see variations in the hair along length (thus, versus time). Using WDS EPMA. I knew the approximate levels (thru some technique like INAA or ICPMS or XRF, I forget) and it was high ppb or low ppm (again, I forget the details). But I could never put enough current (I probably went up to 10 or 20 nA) to see anything.
John
==============================Original Headers============================== 5, 25 -- From johnf-at-geology.wisc.edu Wed Jan 17 07:12:42 2007 5, 25 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0HDCfcB014198 5, 25 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jan 2007 07:12:42 -0600 5, 25 -- Received: from localhost (localhost [127.0.0.1]) 5, 25 -- by localhost (Postfix) with ESMTP id 134F720D31; 5, 25 -- Wed, 17 Jan 2007 07:12:40 -0600 (CST) 5, 25 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) 5, 25 -- by localhost (geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 5, 25 -- with ESMTP id 27330-08; Wed, 17 Jan 2007 07:12:34 -0600 (CST) 5, 25 -- Received: from [192.168.1.103] (24-183-52-127.dhcp.mdsn.wi.charter.com [24.183.52.127]) 5, 25 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 5, 25 -- (No client certificate requested) 5, 25 -- by ice.geology.wisc.edu (Postfix) with ESMTP id 5B2A320D08; 5, 25 -- Wed, 17 Jan 2007 07:12:34 -0600 (CST) 5, 25 -- Mime-Version: 1.0 5, 25 -- Message-Id: {p06210201c1d3cfb242f0-at-[192.168.0.122]} 5, 25 -- In-Reply-To: {200701170205.l0H2595J030194-at-ns.microscopy.com} 5, 25 -- References: {200701170205.l0H2595J030194-at-ns.microscopy.com} 5, 25 -- Date: Wed, 17 Jan 2007 07:14:58 -0600 5, 25 -- To: chappell.mark-at-epa.gov, microscopy-at-microscopy.com 5, 25 -- From: John Fournelle {johnf-at-geology.wisc.edu} 5, 25 -- Subject: Re: SEM/EDX of bird feather 5, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 25 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu ==============================End of - Headers==============================
==============================Original Headers============================== 18, 26 -- From ph2-at-sprynet.com Wed Jan 17 10:03:46 2007 18, 26 -- Received: from elasmtp-kukur.atl.sa.earthlink.net (elasmtp-kukur.atl.sa.earthlink.net [209.86.89.65]) 18, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0HG3kNk009005 18, 26 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jan 2007 10:03:46 -0600 18, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 18, 26 -- s=dk20050327; d=sprynet.com; 18, 26 -- b=tBJkEKzzh1IzjTxhOotRSKxWhIDpta24Q7RcnMnOCMDojh+ZqkmliVFRswecmYB1; 18, 26 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-MimeOLE:Thread-Index:In-Reply-To:Message-ID:X-ELNK-Trace:X-Originating-IP; 18, 26 -- Received: from [75.61.18.94] (helo=user915fa8f284) 18, 26 -- by elasmtp-kukur.atl.sa.earthlink.net with asmtp (Exim 4.34) 18, 26 -- id 1H7DGL-0000o2-J8; Wed, 17 Jan 2007 11:03:45 -0500 18, 26 -- From: "Tony Havics" {ph2-at-sprynet.com} 18, 26 -- To: {johnf-at-geology.wisc.edu} , {microscopy-at-microscopy.com} 18, 26 -- Subject: RE: [Microscopy] Re: SEM/EDX of bird feather 18, 26 -- Date: Wed, 17 Jan 2007 11:03:41 -0500 18, 26 -- MIME-Version: 1.0 18, 26 -- Content-Type: text/plain; 18, 26 -- charset="us-ascii" 18, 26 -- Content-Transfer-Encoding: 7bit 18, 26 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 18, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 18, 26 -- Thread-Index: Acc6OnDmWnJkLwdaTQCNI5up6obfkgAFThXg 18, 26 -- In-Reply-To: {200701171321.l0HDLlSR028784-at-ns.microscopy.com} 18, 26 -- Message-ID: {E1H7DGL-0000o2-J8-at-elasmtp-kukur.atl.sa.earthlink.net} 18, 26 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f9d351a46f1ff38ab561926f01341a0e9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 18, 26 -- X-Originating-IP: 75.61.18.94 ==============================End of - Headers==============================
McCrone, Walter, and Michael Bayard: Individualization of Hair. Microscope 47(3):129-133. 1999.
Good article. Ion microprobe.
Walter passed away a few years ago, but Mike Bayard is still around (Bayard consulting).
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
This message is from pH2. This message and any attachments may contain legally privileged or confidential information, and are intended only for the individual or entity identified above as the addressee. If you are not the addressee, or if this message has been addressed to you in error, you are not authorized to read, copy, or distribute this message and any attachments, and we ask that you please delete this message and attachments (including all copies) and notify the sender by return e-mail or by phone at 317-718-7020. Delivery of this message and any attachments to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or a privilege. All personal messages express views only of the sender, which are not to be attributed to pH2 and may not be copied or distributed without this statement.
-----Original Message----- X-from: johnf-at-geology.wisc.edu [mailto:johnf-at-geology.wisc.edu] Sent: Wednesday, January 17, 2007 8:22 AM To: ph2-at-sprynet.com
} } } Question: I want to map the Hg in a contaminated bird feather using } SEM/EDX/WDS. What's the best way to prepare the sample to minimize } charging?
When you say "contaminated" do you mean massive contamination, millions of times greater than what a human would get in their hair (thru metabolism) eating fish that had "high" levels from Hg used in adjacent gold mining/extraction? Only in that case would an electron-based microanalytical technique be of any use.
I attempted to measure Hg in hair from native people from Brazil who lived near gold extraction areas.The idea was to see if we could see variations in the hair along length (thus, versus time). Using WDS EPMA. I knew the approximate levels (thru some technique like INAA or ICPMS or XRF, I forget) and it was high ppb or low ppm (again, I forget the details). But I could never put enough current (I probably went up to 10 or 20 nA) to see anything.
John
==============================Original Headers============================== 5, 25 -- From johnf-at-geology.wisc.edu Wed Jan 17 07:12:42 2007 5, 25 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0HDCfcB014198 5, 25 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jan 2007 07:12:42 -0600 5, 25 -- Received: from localhost (localhost [127.0.0.1]) 5, 25 -- by localhost (Postfix) with ESMTP id 134F720D31; 5, 25 -- Wed, 17 Jan 2007 07:12:40 -0600 (CST) 5, 25 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) 5, 25 -- by localhost (geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 5, 25 -- with ESMTP id 27330-08; Wed, 17 Jan 2007 07:12:34 -0600 (CST) 5, 25 -- Received: from [192.168.1.103] (24-183-52-127.dhcp.mdsn.wi.charter.com [24.183.52.127]) 5, 25 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 5, 25 -- (No client certificate requested) 5, 25 -- by ice.geology.wisc.edu (Postfix) with ESMTP id 5B2A320D08; 5, 25 -- Wed, 17 Jan 2007 07:12:34 -0600 (CST) 5, 25 -- Mime-Version: 1.0 5, 25 -- Message-Id: {p06210201c1d3cfb242f0-at-[192.168.0.122]} 5, 25 -- In-Reply-To: {200701170205.l0H2595J030194-at-ns.microscopy.com} 5, 25 -- References: {200701170205.l0H2595J030194-at-ns.microscopy.com} 5, 25 -- Date: Wed, 17 Jan 2007 07:14:58 -0600 5, 25 -- To: chappell.mark-at-epa.gov, microscopy-at-microscopy.com 5, 25 -- From: John Fournelle {johnf-at-geology.wisc.edu} 5, 25 -- Subject: Re: SEM/EDX of bird feather 5, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 25 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu ==============================End of - Headers==============================
==============================Original Headers============================== 20, 26 -- From ph2-at-sprynet.com Wed Jan 17 10:19:49 2007 20, 26 -- Received: from elasmtp-kukur.atl.sa.earthlink.net (elasmtp-kukur.atl.sa.earthlink.net [209.86.89.65]) 20, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0HGJm4f020222 20, 26 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jan 2007 10:19:48 -0600 20, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 20, 26 -- s=dk20050327; d=sprynet.com; 20, 26 -- b=j37OA7fofM46Bxvdk/AJjcToLbIYpxUbWPBhBeMlbU3bmT9mNy7DHXey1mELx3Q6; 20, 26 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-MimeOLE:Thread-Index:In-Reply-To:Message-ID:X-ELNK-Trace:X-Originating-IP; 20, 26 -- Received: from [75.61.18.94] (helo=user915fa8f284) 20, 26 -- by elasmtp-kukur.atl.sa.earthlink.net with asmtp (Exim 4.34) 20, 26 -- id 1H7DVr-0000Gl-Ck; Wed, 17 Jan 2007 11:19:47 -0500 20, 26 -- From: "Tony Havics" {ph2-at-sprynet.com} 20, 26 -- To: {johnf-at-geology.wisc.edu} , {microscopy-at-microscopy.com} 20, 26 -- Subject: RE: [Microscopy] Re: SEM/EDX of bird feather 20, 26 -- Date: Wed, 17 Jan 2007 11:19:43 -0500 20, 26 -- MIME-Version: 1.0 20, 26 -- Content-Type: text/plain; 20, 26 -- charset="us-ascii" 20, 26 -- Content-Transfer-Encoding: 7bit 20, 26 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 20, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 20, 26 -- Thread-Index: Acc6OnDmWnJkLwdaTQCNI5up6obfkgAGG2UQ 20, 26 -- In-Reply-To: {200701171321.l0HDLlSR028784-at-ns.microscopy.com} 20, 26 -- Message-ID: {E1H7DVr-0000Gl-Ck-at-elasmtp-kukur.atl.sa.earthlink.net} 20, 26 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f92216719cbc12cfa5f0f1f9848c34fed8350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 20, 26 -- X-Originating-IP: 75.61.18.94 ==============================End of - Headers==============================
The maximum voltage from the HV transformer is 2800 volts so it would be safe to set the control knob shaft to the fully clockwise position and place the knob at the 2.8 kV end of the scale. For reference, this is a very old coater (discontinued in around 1988) but some parts are still available. For further support please contact our US distributor Energy Beam Sciences (http://www.quorumtech.com/contact_us/usa.htm).
You can also find a download of the E5100 manual on the technical support pages of our website (http://www.quorumtech.com/Tech_Support/polaron-range-technical-support. htm)
I hope this helps.
Best regards,
Mike Wombwell Quorum Technologies Newhaven, East Sussex, UK Tel: +44(0)1273 510535 Fax: +44(0)1273 510536 mike.wombwell-at-quorumtech.com http://www.quorumtech.com
E & O E
-----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: 17 January 2007 15:44 To: Mike Wombwell
Dear Listers,
While our main sputter coater is out for a much-needed overhaul, we are relying on a backup coater, namely a Polaron E5100. The unit is operating beautifully, except that the voltage dial has worked loose at some point and I can no longer tell where the zero point, and thus the proper operating voltage, is located on the scale. This is because the scale starts at 2.0, not zero, and I have no reference to line up the indicator with. I tried looking at the set-screw scar on the knob shaft, but it didn't help. Am I missing something obvious? (Wouldn't be the first time.....)
f you will go to http://www.emc.missouri.edu/lookatthis.htm to see the images of this coater hopefully this will make sense.
Can someone with a similar unit kindly let me know about where the zero point would be on this machine? I can get it to coat by trial and error, but it would be nice to have a baseline to experiment from.
Many thanks!
Searching for zero, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 9, 23 -- From TindallR-at-missouri.edu Wed Jan 17 09:39:47 2007 9, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0HFdlGO018946 9, 23 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jan 2007 09:39:47 -0600 9, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 9, 23 -- Wed, 17 Jan 2007 09:39:47 -0600 9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 23 -- Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="us-ascii" 9, 23 -- Subject: Polaron E5100 sputter coater question 9, 23 -- Date: Wed, 17 Jan 2007 09:39:46 -0600 9, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68CF1-at-UM-XMAIL08.um.umsystem.edu} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: Polaron E5100 sputter coater question 9, 23 -- Thread-Index: Acc6TbbzCo8D3MqPS+a3y10wR2ngWQ== 9, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 9, 23 -- To: {microscopy-at-microscopy.com} 9, 23 -- X-OriginalArrivalTime: 17 Jan 2007 15:39:47.0209 (UTC) FILETIME=[B7B14790:01C73A4D] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0HFdlGO018946 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 24 -- From mike.wombwell-at-quorumtech.com Wed Jan 17 10:21:13 2007 23, 24 -- Received: from thb-mta-10.emailfiltering.com (thb-mta-10.emailfiltering.com [213.166.4.221]) 23, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0HGLAhi021429 23, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Jan 2007 10:21:11 -0600 23, 24 -- Received: from host213-123-204-94.in-addr.btopenworld.com ([213.123.204.94]) 23, 24 -- by thb-mta-10.emailfiltering.com with emfmta (version 3.2.1.3113.11.rd-3.2.2-libc2.3.1) vanilla id 174099874 23, 24 -- ; Wed, 17 Jan 2007 16:21:06 +0000 23, 24 -- Content-class: urn:content-classes:message 23, 24 -- Subject: RE: [Microscopy] Polaron E5100 sputter coater question 23, 24 -- MIME-Version: 1.0 23, 24 -- Content-Type: text/plain; 23, 24 -- charset="us-ascii" 23, 24 -- Date: Wed, 17 Jan 2007 16:24:41 -0000 23, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 23, 24 -- Message-ID: {4287884323A072458FB10232AA8FDB39293848-at-server01.QuorumTechnologiesLtd.local} 23, 24 -- X-MS-Has-Attach: 23, 24 -- X-MS-TNEF-Correlator: 23, 24 -- Thread-Topic: [Microscopy] Polaron E5100 sputter coater question 23, 24 -- Thread-Index: Acc6TsbyCrZmWbmeQWCcQtMDRN+h8wAAlovg 23, 24 -- From: "Mike Wombwell" {mike.wombwell-at-quorumtech.com} 23, 24 -- To: {TindallR-at-missouri.edu} 23, 24 -- Cc: {Microscopy-at-microscopy.com} 23, 24 -- Content-Transfer-Encoding: 8bit 23, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0HGLAhi021429 ==============================End of - Headers==============================
I would also try to use laboratory micro-XRF before using a synchrotron source. The advantages against common SEM excitation are much better detection limits (because there is no bremsstrahlung in spectra background) and no need of vacuum. I think, micro-XRF meets the needs of your analysis goals.
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==============================Original Headers============================== 8, 19 -- From eggert-at-mikroanalytik.de Wed Jan 17 10:22:15 2007 8, 19 -- Received: from smtp-out.kontent.com (smtp-out001.kontent.com [81.88.40.215]) 8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0HGME90024076 8, 19 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jan 2007 10:22:14 -0600 8, 19 -- Received: from mikroanalytik.de (p54BDC949.dip0.t-ipconnect.de [84.189.201.73]) 8, 19 -- by smtp-out.kontent.com (Postfix) with ESMTP id 7C0A137C197; 8, 19 -- Wed, 17 Jan 2007 17:22:09 +0100 (CET) 8, 19 -- Message-ID: {45AE4CD6.2040607-at-mikroanalytik.de} 8, 19 -- Date: Wed, 17 Jan 2007 17:20:38 +0100 8, 19 -- From: Frank Eggert {eggert-at-mikroanalytik.de} 8, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; de-DE; rv:1.4) Gecko/20030619 Netscape/7.1 (ax) 8, 19 -- X-Accept-Language: de, de-de, en 8, 19 -- MIME-Version: 1.0 8, 19 -- To: microscopy-at-microscopy.com, chappell.mark-at-epa.gov 8, 19 -- Subject: Re: [Microscopy] FW: AskAMicroscopist: SEM/EDX of bird feather 8, 19 -- References: {200701171600.l0HG06OH001278-at-ns.microscopy.com} 8, 19 -- In-Reply-To: {200701171600.l0HG06OH001278-at-ns.microscopy.com} 8, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 8, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Your link didn't work, but I was able to get to the manual with http://www.quorumtech.com/Manuals/E5100-manual.pdf. Thanks very much for this. I'm posting this to the listserve, in case others have this problem also.
I think we've got the problem solved with your suggestion of setting the maximum setting at 2.8 on the dial. Full counterclockwise with this pot then comes out at about 3:00, as Richard Kucklinski also noted in a off-list posting to me. This makes sense now.
I'm suspecting an argon leak, since with the argon turned fully OFF, I'm still getting } 40 mA coating current with the dial set at 2.0-2.2. I'll experiment around with it to get it fine-tuned a bit better, but it's coating reliably now.
Many thanks to all who emailed and phoned. What a resource!!
Sent: Wednesday, January 17, 2007 10:22 AM To: Tindall, Randy D.
Dear Randy,
The maximum voltage from the HV transformer is 2800 volts so it would be safe to set the control knob shaft to the fully clockwise position and place the knob at the 2.8 kV end of the scale. For reference, this is a very old coater (discontinued in around 1988) but some parts are still available. For further support please contact our US distributor Energy Beam Sciences (http://www.quorumtech.com/contact_us/usa.htm).
You can also find a download of the E5100 manual on the technical support pages of our website (http://www.quorumtech.com/Tech_Support/polaron-range-technical-support. htm)
I hope this helps.
Best regards,
Mike Wombwell Quorum Technologies Newhaven, East Sussex, UK Tel: +44(0)1273 510535 Fax: +44(0)1273 510536 mike.wombwell-at-quorumtech.com http://www.quorumtech.com
E & O E
-----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: 17 January 2007 15:44 To: Mike Wombwell
Dear Listers,
While our main sputter coater is out for a much-needed overhaul, we are relying on a backup coater, namely a Polaron E5100. The unit is operating beautifully, except that the voltage dial has worked loose at some point and I can no longer tell where the zero point, and thus the proper operating voltage, is located on the scale. This is because the scale starts at 2.0, not zero, and I have no reference to line up the indicator with. I tried looking at the set-screw scar on the knob shaft, but it didn't help. Am I missing something obvious? (Wouldn't be the first time.....)
f you will go to http://www.emc.missouri.edu/lookatthis.htm to see the images of this coater hopefully this will make sense.
Can someone with a similar unit kindly let me know about where the zero point would be on this machine? I can get it to coat by trial and error, but it would be nice to have a baseline to experiment from.
Many thanks!
Searching for zero, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 9, 23 -- From TindallR-at-missouri.edu Wed Jan 17 09:39:47 2007 9, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0HFdlGO018946 9, 23 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jan 2007 09:39:47 -0600 9, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 9, 23 -- Wed, 17 Jan 2007 09:39:47 -0600 9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 23 -- Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="us-ascii" 9, 23 -- Subject: Polaron E5100 sputter coater question 9, 23 -- Date: Wed, 17 Jan 2007 09:39:46 -0600 9, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68CF1-at-UM-XMAIL08.um.umsystem.edu} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: Polaron E5100 sputter coater question 9, 23 -- Thread-Index: Acc6TbbzCo8D3MqPS+a3y10wR2ngWQ== 9, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 9, 23 -- To: {microscopy-at-microscopy.com} 9, 23 -- X-OriginalArrivalTime: 17 Jan 2007 15:39:47.0209 (UTC) FILETIME=[B7B14790:01C73A4D] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0HFdlGO018946 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 24 -- From mike.wombwell-at-quorumtech.com Wed Jan 17 10:21:13 2007 23, 24 -- Received: from thb-mta-10.emailfiltering.com (thb-mta-10.emailfiltering.com [213.166.4.221]) 23, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0HGLAhi021429 23, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Jan 2007 10:21:11 -0600 23, 24 -- Received: from host213-123-204-94.in-addr.btopenworld.com ([213.123.204.94]) 23, 24 -- by thb-mta-10.emailfiltering.com with emfmta (version 3.2.1.3113.11.rd-3.2.2-libc2.3.1) vanilla id 174099874 23, 24 -- ; Wed, 17 Jan 2007 16:21:06 +0000 23, 24 -- Content-class: urn:content-classes:message 23, 24 -- Subject: RE: [Microscopy] Polaron E5100 sputter coater question 23, 24 -- MIME-Version: 1.0 23, 24 -- Content-Type: text/plain; 23, 24 -- charset="us-ascii" 23, 24 -- Date: Wed, 17 Jan 2007 16:24:41 -0000 23, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 23, 24 -- Message-ID: {4287884323A072458FB10232AA8FDB39293848-at-server01.QuorumTechnologiesLtd.l ocal} 23, 24 -- X-MS-Has-Attach: 23, 24 -- X-MS-TNEF-Correlator: 23, 24 -- Thread-Topic: [Microscopy] Polaron E5100 sputter coater question 23, 24 -- Thread-Index: Acc6TsbyCrZmWbmeQWCcQtMDRN+h8wAAlovg 23, 24 -- From: "Mike Wombwell" {mike.wombwell-at-quorumtech.com} 23, 24 -- To: {TindallR-at-missouri.edu} 23, 24 -- Cc: {Microscopy-at-microscopy.com} 23, 24 -- Content-Transfer-Encoding: 8bit 23, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0HGLAhi021429 ==============================End of - Headers==============================
==============================Original Headers============================== 36, 26 -- From TindallR-at-missouri.edu Wed Jan 17 10:44:53 2007 36, 26 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 36, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0HGiquc021008 36, 26 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jan 2007 10:44:53 -0600 36, 26 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 36, 26 -- Wed, 17 Jan 2007 10:44:52 -0600 36, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 36, 26 -- Content-class: urn:content-classes:message 36, 26 -- MIME-Version: 1.0 36, 26 -- Content-Type: text/plain; 36, 26 -- charset="us-ascii" 36, 26 -- Subject: RE: [Microscopy] RE: Polaron E5100 sputter coater question 36, 26 -- Date: Wed, 17 Jan 2007 10:44:51 -0600 36, 26 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68CF3-at-UM-XMAIL08.um.umsystem.edu} 36, 26 -- in-reply-to: {200701171622.l0HGMF4f024191-at-ns.microscopy.com} 36, 26 -- X-MS-Has-Attach: 36, 26 -- X-MS-TNEF-Correlator: 36, 26 -- Thread-Topic: [Microscopy] RE: Polaron E5100 sputter coater question 36, 26 -- Thread-Index: Acc6U6huRQGSU+asQaSz9+NIox/asQAAclSA 36, 26 -- References: {200701171622.l0HGMF4f024191-at-ns.microscopy.com} 36, 26 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 36, 26 -- To: {mike.wombwell-at-quorumtech.com} 36, 26 -- Cc: {microscopy-at-microscopy.com} 36, 26 -- X-OriginalArrivalTime: 17 Jan 2007 16:44:52.0515 (UTC) FILETIME=[CF6FAF30:01C73A56] 36, 26 -- Content-Transfer-Encoding: 8bit 36, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0HGiquc021008 ==============================End of - Headers==============================
Dear Mark, I have done this in the past, for the Royal Museum in Victoria, BC. They were interested in the contamination of feathers and fur in old stuffed animals and birds, because mercury and arsenic used to be used to preserve museum samples many years ago and the museum wanted to know if these exhibits were contaminated with heavy elements before they were put on display or exposed them to the public. The heavy elements were expected to be applied to the feathers, so the amounts were large, if they were there. I used the SEM in variable pressure mode, because fluffy things are so difficult to coat effectively, and just scanned around with the back-scattered detector, looking for bright things. Any I found I took a picture of and analysed with EDX. I have also detected elevated levels of copper in someone's hair using SEM+EDX. Looking for smaller amounts of heavy elements or organically-bound material in the interior of the sample would require the sample to be mounted in epoxy, polished, carbon-coated and then careful WDX analysis. Good luck, Mary Mager Electron Microscopist Materials Eng. UBC #419 - 6350 Stores Rd. Vancouver, B.C. V6T 1Z4 CANADA Phone: 604-822-5648 Fax: 604-822-3619 email: mager-at-interchange.ubc.ca
-----Original Message----- X-from: chappell.mark-at-epa.gov [mailto:chappell.mark-at-epa.gov] Sent: Tuesday, January 16, 2007 6:06 PM To: mager-at-interchange.ubc.ca
This Question was submitted to Ask-A-Microscopist by (chappell.mark-at-epa.gov) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, January 16, 2007 at 13:59:24 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both chappell.mark-at-epa.gov as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: chappell.mark-at-epa.gov Name: Mark Chappell
Organization: U.S. EPA
Education: Graduate College
Location: Cincinnati, OH
Title: SEM/EDX of bird feather
Question: I want to map the Hg in a contaminated bird feather using SEM/EDX/WDS. What's the best way to prepare the sample to minimize charging?
Actually, the work done by Dr. McCrone in the early 1970's on a forensic hair case from Detroit, used an ion microprobe/secondary ion mass spectrometry (SIMS) rather than neutron activation analysis (NAA). We have abandoned the idea of mapping the trace elemental concentrations along the shaft of the hair due to many difficulties with that approach. More recently we concentrated trace elements in hairs by low temperature ashing (LTA) and used SIMS to identify and measure trace element concentrations in the ash. The quantification isn't very accurate and the levels need to be compared with good positive and negative controls because there is no baseline data for comparison. In other words, you might determine that a questioned hair contains levels of lead, for example, greater than expected in hairs from normal people where they have not received significant exposure to lead. We presume the same approach could be used for feathers. Of course, the other option is to use a bulk method such as XRF at Argonne. For more information contact Dick Bisbing off-line at dbisbing-at-mccrone.com.
Regards,
Elaine ********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
-----Original Message----- X-from: chappell.mark-at-epa.gov [mailto:chappell.mark-at-epa.gov] Sent: Tuesday, January 16, 2007 8:06 PM To: Elaine F. Schumacher
This Question was submitted to Ask-A-Microscopist by (chappell.mark-at-epa.gov) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, January 16, 2007 at 13:59:24 Remember to consider the Grade/Age of the student when considering the Question ------------------------------------------------------------------------ --- Please reply to both chappell.mark-at-epa.gov as well as to the Microscopy Listserver ------------------------------------------------------------------------ ---
Email: chappell.mark-at-epa.gov Name: Mark Chappell
Organization: U.S. EPA
Education: Graduate College
Location: Cincinnati, OH
Title: SEM/EDX of bird feather
Question: I want to map the Hg in a contaminated bird feather using SEM/EDX/WDS. What's the best way to prepare the sample to minimize charging?
If I remember correctly the top end of the E5000 series variac scale was 2.8kV. Best results, if you do not want to cook your specimen, are achieved at 20mA and around 800 volts in other words about 1/3 to 1/2 scale from minimum.
Hope this helps?
Steve Chapman Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
----- Original Message ----- X-from: {TindallR-at-missouri.edu} To: {protrain-at-emcourses.com} Sent: Wednesday, January 17, 2007 4:45 PM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sue.tyler-at-noaa.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sue.tyler-at-noaa.gov Name: Sue Tyler
Organization: Cooperative Oxford Laboratory
Title-Subject: [Filtered] TEM and ecological assesment
Question: My laboratory is changing directions and shifting from disease research to ecosystem health assessment. The past users of the TEM lab have asked me to justify the need for TEM in this new line of work. I am the technician and not aware of current research in the field of ecosystem health. I have received a few papers off the internet. There are a few institutions who use TEM in that field, but I was wondering if there are anymore out there? Can you point me in the right direction?
With the wealth of knowlege in "microscopy land" I am sure someone can help me.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both klangwor-at-uoregon.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: klangwor-at-uoregon.edu Name: Kurt Langworthy
Organization: University of Oregon
Title-Subject: [Filtered] EBL/ PMMA Mask
Question: All, I'm looking for a gold etching recipt that will allow me to use PMMA as a mask. KCN looks promising, but is highly toxic. Does anyone have any better ideas? Thanks, Kurt
And along a completely different line, we've been asked if we can do a "spark emission test". Since I don't know what that is, and Google hasn't helped, I don't know if we can or not.
Has anybody heard of this test? Seems to be a way of checking sample composition at concentrations below those attainable by EDS, but that's about all I can tell.
Sorry if this isn't microscopy-related, but I don't even know that, at this point. We'll see what the collective comes up with on this one!
Thanks to all.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 7, 23 -- From TindallR-at-missouri.edu Thu Jan 18 10:12:19 2007 7, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0IGCJAl020222 7, 23 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 10:12:19 -0600 7, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 7, 23 -- Thu, 18 Jan 2007 10:12:19 -0600 7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="us-ascii" 7, 23 -- Subject: Spark emission test? 7, 23 -- Date: Thu, 18 Jan 2007 10:12:18 -0600 7, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68D0E-at-UM-XMAIL08.um.umsystem.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- Thread-Topic: Spark emission test? 7, 23 -- Thread-Index: Acc7G207OlT1I53OTyafRC/FuYiYDg== 7, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 7, 23 -- To: {microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 18 Jan 2007 16:12:19.0651 (UTC) FILETIME=[6DDA0930:01C73B1B] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0IGCJAl020222 ==============================End of - Headers==============================
I don't have one, but am familiar with the technique. It is not uncommon in foundry and other metallurgical areas. The instrument generates an electric arc to a (typically metallic) specimen and "looks" at the resulting emission spectrum. It is not useful for microanalysis, but the larger analysis volume works pretty well for average compositions. I don't remember the detection limit(s), but it is pretty low. Another advantage is that it is quick and there is little sample preparation required.
Hope this brief response is helpful... Woody White BWXT Services
-----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: Thursday, January 18, 2007 11:13 AM To: White, Woody N.
And along a completely different line, we've been asked if we can do a "spark emission test". Since I don't know what that is, and Google hasn't helped, I don't know if we can or not.
Has anybody heard of this test? Seems to be a way of checking sample composition at concentrations below those attainable by EDS, but that's about all I can tell.
Sorry if this isn't microscopy-related, but I don't even know that, at this point. We'll see what the collective comes up with on this one!
Thanks to all.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 7, 23 -- From TindallR-at-missouri.edu Thu Jan 18 10:12:19 2007 7, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0IGCJAl020222 7, 23 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 10:12:19 -0600 7, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 7, 23 -- Thu, 18 Jan 2007 10:12:19 -0600 7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="us-ascii" 7, 23 -- Subject: Spark emission test? 7, 23 -- Date: Thu, 18 Jan 2007 10:12:18 -0600 7, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68D0E-at-UM-XMAIL08.um.umsystem.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- Thread-Topic: Spark emission test? 7, 23 -- Thread-Index: Acc7G207OlT1I53OTyafRC/FuYiYDg== 7, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 7, 23 -- To: {microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 18 Jan 2007 16:12:19.0651 (UTC) FILETIME=[6DDA0930:01C73B1B] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0IGCJAl020222 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 27 -- From nwwhite-at-bwxt.com Thu Jan 18 10:24:47 2007 18, 27 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 18, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0IGOkGM031352 18, 27 -- for {microscopy-at-msa.microscopy.com} ; Thu, 18 Jan 2007 10:24:46 -0600 18, 27 -- Received: from ([131.184.13.224]) 18, 27 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.3675195; 18, 27 -- Thu, 18 Jan 2007 11:24:20 -0500 18, 27 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); 18, 27 -- Thu, 18 Jan 2007 11:24:20 -0500 18, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 18, 27 -- Content-class: urn:content-classes:message 18, 27 -- MIME-Version: 1.0 18, 27 -- Content-Type: text/plain; 18, 27 -- charset="us-ascii" 18, 27 -- Subject: RE: [Microscopy] Spark emission test? 18, 27 -- Date: Thu, 18 Jan 2007 11:24:20 -0500 18, 27 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB703D110-at-BWXSPO01.BWXS.BWXTECH.NET} 18, 27 -- X-MS-Has-Attach: 18, 27 -- X-MS-TNEF-Correlator: 18, 27 -- Thread-Topic: [Microscopy] Spark emission test? 18, 27 -- Thread-Index: Acc7G5uvDzuNapjlSNqW5XCH7jMkNAAACgIg 18, 27 -- From: "White, Woody N." {NWWhite-at-bwxt.com} 18, 27 -- To: {TindallR-at-missouri.edu} , 18, 27 -- "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 18, 27 -- X-OriginalArrivalTime: 18 Jan 2007 16:24:20.0707 (UTC) FILETIME=[1BA27330:01C73B1D] 18, 27 -- Content-Transfer-Encoding: 8bit 18, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0IGOkGM031352 ==============================End of - Headers==============================
Randy, While this could be some type of laser emission spectroscopy test, I have also read about spark test for metals. As I remember it different steels and other metals will shoot out different colored, shaped, morphology , behavior (you catch the drift ?) sparks when touch to a grind wheel. It's been reported that an experienced operator can tell what grade of stainless steel based on the properties of sparks.
Never tried this myself.......... Too hard to get the grinding wheel under my stereomicroscope......
TindallR-at-missouri .edu To: frank.karl-at-degussa.com cc: 01/18/2007 11:14 Subject: [Microscopy] Spark emission test? AM Please respond to TindallR
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
And along a completely different line, we've been asked if we can do a "spark emission test". Since I don't know what that is, and Google hasn't helped, I don't know if we can or not.
Has anybody heard of this test? Seems to be a way of checking sample composition at concentrations below those attainable by EDS, but that's about all I can tell.
Sorry if this isn't microscopy-related, but I don't even know that, at this point. We'll see what the collective comes up with on this one!
Thanks to all.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 7, 23 -- From TindallR-at-missouri.edu Thu Jan 18 10:12:19 2007 7, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0IGCJAl020222 7, 23 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 10:12:19 -0600 7, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 7, 23 -- Thu, 18 Jan 2007 10:12:19 -0600 7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="us-ascii" 7, 23 -- Subject: Spark emission test? 7, 23 -- Date: Thu, 18 Jan 2007 10:12:18 -0600 7, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68D0E-at-UM-XMAIL08.um.umsystem.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- Thread-Topic: Spark emission test? 7, 23 -- Thread-Index: Acc7G207OlT1I53OTyafRC/FuYiYDg== 7, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 7, 23 -- To: {microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 18 Jan 2007 16:12:19.0651 (UTC) FILETIME=[6DDA0930:01C73B1B] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0IGCJAl020222 ==============================End of - Headers==============================
==============================Original Headers============================== 25, 18 -- From frank.karl-at-degussa.com Thu Jan 18 10:33:12 2007 25, 18 -- Received: from framailout1.rz.itson.com (mailout2.degussa.com [149.216.91.173]) 25, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0IGXCkx009902 25, 18 -- for {microscopy-at-msa.microscopy.com} ; Thu, 18 Jan 2007 10:33:12 -0600 25, 18 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [10.88.88.98]) 25, 18 -- by framailout1.rz.itson.com (8.13.4/8.13.4/Debian-3sarge3) with SMTP id l0IGX9ae009353 25, 18 -- for {microscopy-at-msa.microscopy.com} ; Thu, 18 Jan 2007 17:33:09 +0100 25, 18 -- In-Reply-To: {200701181614.l0IGET2g022368-at-ns.microscopy.com} 25, 18 -- Subject: Re: [Microscopy] Spark emission test? 25, 18 -- To: TindallR-at-missouri.edu, microscopy-at-msa.microscopy.com 25, 18 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 25, 18 -- Message-ID: {OFFEA8A4ED.CFFC3950-ON86257267.005A4DCA-85257267.005AEB26-at-degussa.com} 25, 18 -- From: frank.karl-at-degussa.com 25, 18 -- Date: Thu, 18 Jan 2007 11:33:04 -0500 25, 18 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 25, 18 -- 01/18/2007 10:33:11 AM 25, 18 -- MIME-Version: 1.0 25, 18 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Your question of "spark emission test" sounds familiar but I can't put my finger on it. I can think of three techniques that may be what is being asked: Spark Testing, Spark Source Mass Spectrometery, or Optical Emission Spectroscopy.
Spark Testing - this is a technique that simply uses a grinder. A piece of metal is pushed against the grinder. The sparks that fly off are indicative of what the alloy is. I only know of it being used in metal alloys. It would be the kind of test someone dealing with a scrap yard would find useful. It can basically tell you what grade of metal you are dealing with. It takes a fair amount of practice to be able to visually know what alloy the spark pattern is indicating. People that do this test frequently can do quite well with it as far as getting a good handle on what alloy they have in their hand.
Spark Source Mass Spectrometery - This is used for a wide range of samples, but tends to do better with samples that are conductive, although that is not necessarily a limitation. There are two other techniques that are related to this: Laser Ionization Mass Spectrometery and Inductively Coupled Plasma Atomic Emission.
Optical Emission Spectroscopy - I'm not too familiar with this technique, but I seem to recall they will often use a spark source in the process. When they do that, I think this is then called Spark Emission Spectroscopy. You might want to check out Bruker AXS Microanalysis, I believe they make some of these instruments.
Perhaps if you post what they are testing and what they want from the analysis, that may help. I hope I've given you at least some food for thought.
Good luck,
dj
On Thu, 18 Jan 2007, TindallR-at-missouri.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } And along a completely different line, we've been asked if we can do a } "spark emission test". Since I don't know what that is, and Google } hasn't helped, I don't know if we can or not. } } Has anybody heard of this test? Seems to be a way of checking sample } composition at concentrations below those attainable by EDS, but that's } about all I can tell. } } Sorry if this isn't microscopy-related, but I don't even know that, at } this point. We'll see what the collective comes up with on this one! } } Thanks to all. } } Randy } } Randy Tindall } Senior EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } On-line calendar: } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } Week&NavType=Both&Type=TimePlan } } } ==============================Original Headers============================== } 7, 23 -- From TindallR-at-missouri.edu Thu Jan 18 10:12:19 2007 } 7, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) } 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0IGCJAl020222 } 7, 23 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 10:12:19 -0600 } 7, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); } 7, 23 -- Thu, 18 Jan 2007 10:12:19 -0600 } 7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 7, 23 -- Content-class: urn:content-classes:message } 7, 23 -- MIME-Version: 1.0 } 7, 23 -- Content-Type: text/plain; } 7, 23 -- charset="us-ascii" } 7, 23 -- Subject: Spark emission test? } 7, 23 -- Date: Thu, 18 Jan 2007 10:12:18 -0600 } 7, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68D0E-at-UM-XMAIL08.um.umsystem.edu} } 7, 23 -- X-MS-Has-Attach: } 7, 23 -- X-MS-TNEF-Correlator: } 7, 23 -- Thread-Topic: Spark emission test? } 7, 23 -- Thread-Index: Acc7G207OlT1I53OTyafRC/FuYiYDg== } 7, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 7, 23 -- To: {microscopy-at-microscopy.com} } 7, 23 -- X-OriginalArrivalTime: 18 Jan 2007 16:12:19.0651 (UTC) FILETIME=[6DDA0930:01C73B1B] } 7, 23 -- Content-Transfer-Encoding: 8bit } 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0IGCJAl020222 } ==============================End of - Headers============================== }
==============================Original Headers============================== 13, 19 -- From dljones-at-bestweb.net Thu Jan 18 11:15:31 2007 13, 19 -- Received: from vms044pub.verizon.net (vms044pub.verizon.net [206.46.252.44]) 13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0IHFULY021977 13, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 11:15:31 -0600 13, 19 -- Received: from localhost ([71.247.249.221]) 13, 19 -- by vms044.mailsrvcs.net (Sun Java System Messaging Server 6.2-6.01 (built Apr 13, 19 -- 3 2006)) with ESMTPA id {0JC200NM7QL94A26-at-vms044.mailsrvcs.net} for 13, 19 -- Microscopy-at-microscopy.com; Thu, 18 Jan 2007 11:15:14 -0600 (CST) 13, 19 -- Date: Thu, 18 Jan 2007 12:14:16 -0500 (Eastern Standard Time) 13, 19 -- From: "David L. Jones" {dljones-at-bestweb.net} 13, 19 -- Subject: Re: [Microscopy] Spark emission test? 13, 19 -- In-reply-to: {200701181618.l0IGIW0e026577-at-ns.microscopy.com} 13, 19 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 13, 19 -- To: TindallR-at-missouri.edu 13, 19 -- Cc: Microscopy-at-microscopy.com 13, 19 -- Message-id: {Pine.WNT.4.64.0701181137340.3320-at-H-F1} 13, 19 -- MIME-version: 1.0 13, 19 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 13, 19 -- References: {200701181618.l0IGIW0e026577-at-ns.microscopy.com} ==============================End of - Headers==============================
dj is right, when metallurgists say "spark test" they generally mean optical emission spectroscopy (OES). OES can give reliable sulfur and phosphorous (for instance) readings at low concentrations I would never have the chutzpa to report from EDS.
The grinding wheel test can distinguish high-carbon (short sparks, in dense clusters) from low-carbon steels (few sparks, long, and not in clusters). I do not think it can distinguish whether any other alloying elements are present or not. There is a great ASTM document - STP 550 "Nondestructive Rapid Identification of Metals and Alloys by Spot Test" from 1974 that covers chemical spot tests for qualitative alloy i.d. but that is 'way off-topic.
Regards, Andrew
At 11:16 AM 1/18/2007, you wrote:
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==============================Original Headers============================== 8, 36 -- From werner-at-rosharon.oilfield.slb.com Thu Jan 18 13:55:02 2007 8, 36 -- Received: from mail.slb.com (nammta01.sugar-land.nam.slb.com [163.188.150.130]) 8, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0IJt2Wl003343 8, 36 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 13:55:02 -0600 8, 36 -- Received: from pmxchannel_int-daemon.nammta01.sugar-land.nam.slb.com by 8, 36 -- nammta01.sugar-land.nam.slb.com 8, 36 -- (iPlanet Messaging Server 5.2 Patch 2 (built Jul 14 2004)) 8, 36 -- id {0JC200301XENHX-at-nammta01.sugar-land.nam.slb.com} for 8, 36 -- microscopy-at-microscopy.com; Thu, 18 Jan 2007 19:42:23 +0000 (GMT) 8, 36 -- Received: from usxsl052.slb.atosorigin-asp.com 8, 36 -- (usxsl052.slb.atosorigin-asp.com [199.6.136.167]) 8, 36 -- by nammta01.sugar-land.nam.slb.com 8, 36 -- (iPlanet Messaging Server 5.2 Patch 2 (built Jul 14 2004)) 8, 36 -- with ESMTP id {0JC200G99XEN8P-at-nammta01.sugar-land.nam.slb.com} for 8, 36 -- microscopy-at-microscopy.com; Thu, 18 Jan 2007 19:42:23 +0000 (GMT) 8, 36 -- Received: from pmxchannel-daemon.us085mbx01.slb.atosorigin-asp.com by 8, 36 -- us085mbx01.slb.atosorigin-asp.com 8, 36 -- (Sun Java System Messaging Server 6.2-1 (built Feb 24 2005)) 8, 36 -- id {0JC20092VXEJW500-at-us085mbx01.slb.atosorigin-asp.com} for 8, 36 -- microscopy-at-microscopy.com; Thu, 18 Jan 2007 19:42:19 +0000 (GMT) 8, 36 -- Received: from WERNER1-OFS.rosharon.oilfield.slb.com ([163.188.46.5]) 8, 36 -- by us085mbx01.slb.atosorigin-asp.com 8, 36 -- (Sun Java System Messaging Server 6.2-1 (built Feb 24 2005)) 8, 36 -- with ESMTPSA id {0JC2003Z9XEJQX50-at-us085mbx01.slb.atosorigin-asp.com} for 8, 36 -- microscopy-at-microscopy.com; Thu, 18 Jan 2007 19:42:19 +0000 (GMT) 8, 36 -- Date: Thu, 18 Jan 2007 13:42:18 -0600 8, 36 -- From: Andrew Werner {werner-at-rosharon.oilfield.slb.com} 8, 36 -- Subject: Re: [Microscopy] Re: Spark emission test? 8, 36 -- In-reply-to: {200701181716.l0IHGH9F023234-at-ns.microscopy.com} 8, 36 -- To: microscopy-at-microscopy.com 8, 36 -- Message-id: {6.2.1.2.2.20070118133232.03439d28-at-us1061-pop3.mail.slb.com} 8, 36 -- MIME-version: 1.0 8, 36 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2 8, 36 -- Content-type: text/plain; charset=us-ascii; format=flowed 8, 36 -- Content-transfer-encoding: 7BIT 8, 36 -- References: {200701181716.l0IHGH9F023234-at-ns.microscopy.com} ==============================End of - Headers==============================
In an ancient edition of The Metals Handbook (Amer Soc for Materials, ASM) I found the following :
"The most widely used nonelectrolytic etch is a mixture of equal volumes of 10% ammonium persulfate in water with 10% KCN in water. Although the separate solutions are stable in air, the mixture must be used within a few minutes of mixing. Since this solution and the fumes from it are poisonous, it should be used under a hood (and with other relevant precautions) . . . . . . When swabbed on specimens of the usual gold alloys and palladium alloys the mixture acts rapidly and smoothly. It may also be used on silver and certain nickle alloys. For more resistant alloys, 20% solutions may be employed"
"The same metals can be etched when made the anode in a 5% KCN solution" (I'd suggest a voltage of about 5 V). Also mentioned is electrolytic etching using an a.c. voltage (you can probably use a variac as a source for this, again about 5 volts) with a 20% solution of hydrochloric acid saturated with sodium chloride.
The great advantage of Gold and the other noble metals is their chemical stabilite, and so your task is a non-trivial one.
Good luck, -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-engin.umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 6, 14 -- From bigelow-at-engin.umich.edu Thu Jan 18 13:59:24 2007 6, 14 -- Received: from srvr20.engin.umich.edu (srvr20.engin.umich.edu [141.213.75.22]) 6, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0IJxNTZ009333 6, 14 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 13:59:23 -0600 6, 14 -- Received: from [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 6, 14 -- by srvr20.engin.umich.edu (8.13.6/8.13.6) with ESMTP id l0IJxNcC020136 6, 14 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 14:59:23 -0500 (EST) 6, 14 -- Mime-Version: 1.0 6, 14 -- Message-Id: {p06210200c1d5793b7bb7-at-[141.212.131.221]} 6, 14 -- Date: Thu, 18 Jan 2007 14:59:22 -0500 6, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 6, 14 -- From: Wil Bigelow {bigelow-at-engin.umich.edu} 6, 14 -- Subject: [Microscopy] RE: Etching gold 6, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
This sounds like optical emission arc-spectrometry. It sparks a metal sample and the different light wavelengths are channeled and read to quantify the elements. We use it in our lab for accurate measurements of elemental content in different metal alloys. This technique is considered very accurate, considerably better and more reliable than EDS techniques. Some elements can be detected in the ppm range.
Stu Smalinskas, P.E. Metallurgist SKF Plymouth, Michigan (734) 414-6862
--- frank.karl-at-degussa.com wrote:
} Randy, } While this could be some type of laser emission } spectroscopy test, I have } also read about spark test for metals. As I } remember it different steels } and other metals will shoot out different colored, } shaped, morphology , } behavior (you catch the drift ?) sparks when touch } to a grind wheel. It's } been reported that an experienced operator can tell } what grade of stainless } steel based on the properties of sparks. } } Never tried this myself.......... Too hard to get } the grinding wheel under } my stereomicroscope...... } } } } } --- Randy wrote: } } And along a completely different line, we've been } asked if we can do a } "spark emission test". Since I don't know what that } is, and Google } hasn't helped, I don't know if we can or not. } } Has anybody heard of this test? Seems to be a way } of checking sample } composition at concentrations below those attainable } by EDS, but that's } about all I can tell. } } Sorry if this isn't microscopy-related, but I don't } even know that, at } this point. We'll see what the collective comes up } with on this one! } } Thanks to all. } } Randy } } Randy Tindall } Senior EM Specialist } Electron Microscopy Core Facility---We Do Small } Well! } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } On-line calendar: } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } Week&NavType=Both&Type=TimePlan }
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==============================Original Headers============================== 7, 20 -- From smalinskas-at-yahoo.com Thu Jan 18 14:22:27 2007 7, 20 -- Received: from web34413.mail.mud.yahoo.com (web34413.mail.mud.yahoo.com [66.163.178.162]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0IKMR5h025518 7, 20 -- for {microscopy-at-sparc5.microscopy.com} ; Thu, 18 Jan 2007 14:22:27 -0600 7, 20 -- Received: (qmail 39218 invoked by uid 60001); 18 Jan 2007 20:22:26 -0000 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 20 -- s=s1024; d=yahoo.com; 7, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 20 -- b=JZCRqLAkID61w/jC3In0bdqAW1jS4FD/LY3jw0bryfCxQF+q2vP9FngOg80uM5vKgEnp8Q9++rmbWpADMESwX3ks63T3RBAwdQehrREbEgjl+6MCtcyl0vSQg7XQnVAOAZCkdoks5Aybt+5jFGcuiUGjOogDL50jj/kdRlmyeJo=; 7, 20 -- X-YMail-OSG: VRGraHoVM1mlDkQhOovEDc2WkHKrPO363WKRTxaY 7, 20 -- Received: from [141.151.33.213] by web34413.mail.mud.yahoo.com via HTTP; Thu, 18 Jan 2007 12:22:26 PST 7, 20 -- Date: Thu, 18 Jan 2007 12:22:26 -0800 (PST) 7, 20 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 7, 20 -- Subject: Re: [Microscopy] Re: Spark emission test? 7, 20 -- To: microscopy-at-ns.microscopy.com, tindallr-at-missouri.edu 7, 20 -- In-Reply-To: {200701181634.l0IGYKL8012393-at-ns.microscopy.com} 7, 20 -- MIME-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=iso-8859-1 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- Message-ID: {748483.38927.qm-at-web34413.mail.mud.yahoo.com} ==============================End of - Headers==============================
Many thanks to all who responded to my query about spark emission testing. I have passed on the information to the client who requested it, and even found a local source to do the test----right here in the UM system.
Sorry for cluttering up the list with what turned out to be a non-microscopy issue, but I learned something new.
Next question: is there anything the listserve denizens can't answer?
Thanks again!
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 7, 23 -- From TindallR-at-missouri.edu Thu Jan 18 14:34:50 2007 7, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0IKYoNV004299 7, 23 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 14:34:50 -0600 7, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 7, 23 -- Thu, 18 Jan 2007 14:34:49 -0600 7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="us-ascii" 7, 23 -- Subject: Spark emission test 7, 23 -- Date: Thu, 18 Jan 2007 14:34:49 -0600 7, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68D1F-at-UM-XMAIL08.um.umsystem.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- Thread-Topic: Spark emission test 7, 23 -- Thread-Index: Acc7QBmlkLDae5eJSMug3056bJpIBw== 7, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 7, 23 -- To: {microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 18 Jan 2007 20:34:50.0159 (UTC) FILETIME=[19E2D3F0:01C73B40] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0IKYoNV004299 ==============================End of - Headers==============================
Actually, the grinding wheel test can tell a lot more than one would think. You can tell stainless steels, high speed steels, if the alloy contains cobalt, and more. I used to have an old text that listed the spark patterns for a wide range of alloys. But I haven't been able to find it in years. It was handed out in a technical course on welding I took many years ago at a community college. This was an excellent reference that had several pages of spark patterns in color identifying the various alloys that each spark test indicated. It has to be in color as color is one of the identifiers. If anyone knows of a reference that has an extensive color chart like this, can you please send me the reference? I've been looking for a long time to find this again.
Randy,
You didn't say what the specific test was you have found to be it. Is it indeed OES?
dj
On Thu, 18 Jan 2007, werner-at-rosharon.oilfield.slb.com wrote:
} Date: Thu, 18 Jan 2007 13:59:42 -0600 } From: werner-at-rosharon.oilfield.slb.com } To: dljones-at-bestweb.net } Subject: [Microscopy] Spark emission test? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } dj is right, when metallurgists say "spark test" they generally mean } optical emission spectroscopy (OES). OES can give reliable sulfur and } phosphorous (for instance) readings at low concentrations I would never } have the chutzpa to report from EDS. } } The grinding wheel test can distinguish high-carbon (short sparks, in dense } clusters) from low-carbon steels (few sparks, long, and not in } clusters). I do not think it can distinguish whether any other alloying } elements are present or not. There is a great ASTM document - STP 550 } "Nondestructive Rapid Identification of Metals and Alloys by Spot Test" } from 1974 that covers chemical spot tests for qualitative alloy i.d. but } that is 'way off-topic. } } Regards, } Andrew } } At 11:16 AM 1/18/2007, you wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Randy, } } } } Your question of "spark emission test" sounds familiar but I can't put my } } finger on it. I can think of three techniques that may be what is being } } asked: Spark Testing, Spark Source Mass Spectrometery, or Optical Emission } } Spectroscopy. } } } } Spark Testing - this is a technique that simply uses a grinder. A piece of } } metal is pushed against the grinder. The sparks that fly off are } } indicative of what the alloy is. I only know of it being used in metal } } alloys. It would be the kind of test someone dealing with a scrap yard } } would find useful. It can basically tell you what grade of metal you are } } dealing with. It takes a fair amount of practice to be able to visually } } know what alloy the spark pattern is indicating. People that do this test } } frequently can do quite well with it as far as getting a good handle on } } what alloy they have in their hand. } } } } Spark Source Mass Spectrometery - This is used for a wide range of } } samples, but tends to do better with samples that are conductive, although } } that is not necessarily a limitation. There are two other techniques that } } are related to this: Laser Ionization Mass Spectrometery and Inductively } } Coupled Plasma Atomic Emission. } } } } Optical Emission Spectroscopy - I'm not too familiar with this technique, } } but I seem to recall they will often use a spark source in the process. } } When they do that, I think this is then called Spark Emission } } Spectroscopy. You might want to check out Bruker AXS Microanalysis, I } } believe they make some of these instruments. } } } } Perhaps if you post what they are testing and what they want from the } } analysis, that may help. I hope I've given you at least some food for } } thought. } } } } Good luck, } } } } dj } } } } } } } } On Thu, 18 Jan 2007, TindallR-at-missouri.edu wrote: } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } ---------------------------------------------------------------------------- } } } } } } And along a completely different line, we've been asked if we can do a } } } "spark emission test". Since I don't know what that is, and Google } } } hasn't helped, I don't know if we can or not. } } } } } } Has anybody heard of this test? Seems to be a way of checking sample } } } composition at concentrations below those attainable by EDS, but that's } } } about all I can tell. } } } } } } Sorry if this isn't microscopy-related, but I don't even know that, at } } } this point. We'll see what the collective comes up with on this one! } } } } } } Thanks to all. } } } } } } Randy } } } } } } Randy Tindall } } } Senior EM Specialist } } } Electron Microscopy Core Facility---We Do Small Well! } } } W125 Veterinary Medicine } } } University of Missouri } } } Columbia, MO 65211 } } } Tel: (573) 882-8304 } } } Fax: (573) 884-2227 } } } Email: tindallr-at-missouri.edu } } } Web: http://www.emc.missouri.edu } } } On-line calendar: } } } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } } } Week&NavType=Both&Type=TimePlan } } } } } } } } } ==============================Original } } Headers============================== } } } 7, 23 -- From TindallR-at-missouri.edu Thu Jan 18 10:12:19 2007 } } } 7, 23 -- Received: from um-tsmtpout1.um.umsystem.edu } } (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) } } } 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id l0IGCJAl020222 } } } 7, 23 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 } } 10:12:19 -0600 } } } 7, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) } } by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); } } } 7, 23 -- Thu, 18 Jan 2007 10:12:19 -0600 } } } 7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } } } 7, 23 -- Content-class: urn:content-classes:message } } } 7, 23 -- MIME-Version: 1.0 } } } 7, 23 -- Content-Type: text/plain; } } } 7, 23 -- charset="us-ascii" } } } 7, 23 -- Subject: Spark emission test? } } } 7, 23 -- Date: Thu, 18 Jan 2007 10:12:18 -0600 } } } 7, 23 -- Message-ID: } } {91108EF9255B394CBF8B7E3789814A41C68D0E-at-UM-XMAIL08.um.umsystem.edu} } } } 7, 23 -- X-MS-Has-Attach: } } } 7, 23 -- X-MS-TNEF-Correlator: } } } 7, 23 -- Thread-Topic: Spark emission test? } } } 7, 23 -- Thread-Index: Acc7G207OlT1I53OTyafRC/FuYiYDg== } } } 7, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } } } 7, 23 -- To: {microscopy-at-microscopy.com} } } } 7, 23 -- X-OriginalArrivalTime: 18 Jan 2007 16:12:19.0651 (UTC) } } FILETIME=[6DDA0930:01C73B1B] } } } 7, 23 -- Content-Transfer-Encoding: 8bit } } } 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id l0IGCJAl020222 } } } ==============================End of - } } Headers============================== } } } } } } } } } ==============================Original Headers============================== } } 13, 19 -- From dljones-at-bestweb.net Thu Jan 18 11:15:31 2007 } } 13, 19 -- Received: from vms044pub.verizon.net (vms044pub.verizon.net } } [206.46.252.44]) } } 13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id l0IHFULY021977 } } 13, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 11:15:31 } } -0600 } } 13, 19 -- Received: from localhost ([71.247.249.221]) } } 13, 19 -- by vms044.mailsrvcs.net (Sun Java System Messaging Server } } 6.2-6.01 (built Apr } } 13, 19 -- 3 2006)) with ESMTPA id {0JC200NM7QL94A26-at-vms044.mailsrvcs.net} for } } 13, 19 -- Microscopy-at-microscopy.com; Thu, 18 Jan 2007 11:15:14 -0600 (CST) } } 13, 19 -- Date: Thu, 18 Jan 2007 12:14:16 -0500 (Eastern Standard Time) } } 13, 19 -- From: "David L. Jones" {dljones-at-bestweb.net} } } 13, 19 -- Subject: Re: [Microscopy] Spark emission test? } } 13, 19 -- In-reply-to: {200701181618.l0IGIW0e026577-at-ns.microscopy.com} } } 13, 19 -- X-X-Sender: dljones-at-pop-croton.bestweb.net } } 13, 19 -- To: TindallR-at-missouri.edu } } 13, 19 -- Cc: Microscopy-at-microscopy.com } } 13, 19 -- Message-id: {Pine.WNT.4.64.0701181137340.3320-at-H-F1} } } 13, 19 -- MIME-version: 1.0 } } 13, 19 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed } } 13, 19 -- References: {200701181618.l0IGIW0e026577-at-ns.microscopy.com} } } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 8, 36 -- From werner-at-rosharon.oilfield.slb.com Thu Jan 18 13:55:02 2007 } 8, 36 -- Received: from mail.slb.com (nammta01.sugar-land.nam.slb.com [163.188.150.130]) } 8, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0IJt2Wl003343 } 8, 36 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 13:55:02 -0600 } 8, 36 -- Received: from pmxchannel_int-daemon.nammta01.sugar-land.nam.slb.com by } 8, 36 -- nammta01.sugar-land.nam.slb.com } 8, 36 -- (iPlanet Messaging Server 5.2 Patch 2 (built Jul 14 2004)) } 8, 36 -- id {0JC200301XENHX-at-nammta01.sugar-land.nam.slb.com} for } 8, 36 -- microscopy-at-microscopy.com; Thu, 18 Jan 2007 19:42:23 +0000 (GMT) } 8, 36 -- Received: from usxsl052.slb.atosorigin-asp.com } 8, 36 -- (usxsl052.slb.atosorigin-asp.com [199.6.136.167]) } 8, 36 -- by nammta01.sugar-land.nam.slb.com } 8, 36 -- (iPlanet Messaging Server 5.2 Patch 2 (built Jul 14 2004)) } 8, 36 -- with ESMTP id {0JC200G99XEN8P-at-nammta01.sugar-land.nam.slb.com} for } 8, 36 -- microscopy-at-microscopy.com; Thu, 18 Jan 2007 19:42:23 +0000 (GMT) } 8, 36 -- Received: from pmxchannel-daemon.us085mbx01.slb.atosorigin-asp.com by } 8, 36 -- us085mbx01.slb.atosorigin-asp.com } 8, 36 -- (Sun Java System Messaging Server 6.2-1 (built Feb 24 2005)) } 8, 36 -- id {0JC20092VXEJW500-at-us085mbx01.slb.atosorigin-asp.com} for } 8, 36 -- microscopy-at-microscopy.com; Thu, 18 Jan 2007 19:42:19 +0000 (GMT) } 8, 36 -- Received: from WERNER1-OFS.rosharon.oilfield.slb.com ([163.188.46.5]) } 8, 36 -- by us085mbx01.slb.atosorigin-asp.com } 8, 36 -- (Sun Java System Messaging Server 6.2-1 (built Feb 24 2005)) } 8, 36 -- with ESMTPSA id {0JC2003Z9XEJQX50-at-us085mbx01.slb.atosorigin-asp.com} for } 8, 36 -- microscopy-at-microscopy.com; Thu, 18 Jan 2007 19:42:19 +0000 (GMT) } 8, 36 -- Date: Thu, 18 Jan 2007 13:42:18 -0600 } 8, 36 -- From: Andrew Werner {werner-at-rosharon.oilfield.slb.com} } 8, 36 -- Subject: Re: [Microscopy] Re: Spark emission test? } 8, 36 -- In-reply-to: {200701181716.l0IHGH9F023234-at-ns.microscopy.com} } 8, 36 -- To: microscopy-at-microscopy.com } 8, 36 -- Message-id: {6.2.1.2.2.20070118133232.03439d28-at-us1061-pop3.mail.slb.com} } 8, 36 -- MIME-version: 1.0 } 8, 36 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2 } 8, 36 -- Content-type: text/plain; charset=us-ascii; format=flowed } 8, 36 -- Content-transfer-encoding: 7BIT } 8, 36 -- References: {200701181716.l0IHGH9F023234-at-ns.microscopy.com} } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 20 -- From "dljones-at-bestweb.net"-at-bestweb.net Thu Jan 18 15:57:08 2007 9, 20 -- Received: from mta5.srv.hcvlny.cv.net (mta5.srv.hcvlny.cv.net [167.206.4.200]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0ILv7Cf016738 9, 20 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 15:57:08 -0600 9, 20 -- Received: from localhost (ool-44c62883.dyn.optonline.net [68.198.40.131]) 9, 20 -- by mta5.srv.hcvlny.cv.net 9, 20 -- (Sun Java System Messaging Server 6.2-6.01 (built Apr 3 2006)) 9, 20 -- with ESMTP id {0JC300E9X3MIVOF0-at-mta5.srv.hcvlny.cv.net} for 9, 20 -- microscopy-at-microscopy.com; Thu, 18 Jan 2007 16:56:59 -0500 (EST) 9, 20 -- Date: Thu, 18 Jan 2007 16:48:42 -0500 (Eastern Standard Time) 9, 20 -- From: "David L. Jones" {"dljones-at-bestweb.net"-at-bestweb.net} 9, 20 -- Subject: Re: [Microscopy] Spark emission test? 9, 20 -- In-reply-to: {200701181959.l0IJxg9t010372-at-ns.microscopy.com} 9, 20 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 9, 20 -- Cc: microscopy-at-microscopy.com 9, 20 -- Message-id: {Pine.WNT.4.64.0701181516340.176-at-dljtoshiba} 9, 20 -- MIME-version: 1.0 9, 20 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 9, 20 -- Content-transfer-encoding: 7BIT 9, 20 -- References: {200701181959.l0IJxg9t010372-at-ns.microscopy.com} ==============================End of - Headers==============================
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Email: gregm-at-andersonlabs.com Name: Greg Mann
Title-Subject: [Filtered] Spark Spectroscopy
Question: Optical Emission Spectroscopy (OES) commonly uses an AC spark source to excite a small volume of metallic sample in a coronal discharge. In one application a high frequency AC source strikes an arc with a tungsten electrode. Basically the excited electrons have jumped an electron shell, and on return to their relaxed state, they emit a photon of light characteristic of their source. The light generated is directed into an evacuated spectral box, passed through a grating and separated into its spectral components. Each wavelength of interest (i.e. element of interest) is detected. Solid state detectors have can be nearly continuous in wavelength detection, while traditional photo multipliers are discreet in location, often with greater sensitivity. Other OES techniques may employ a DC arc, glow discharge source, or Inductively coupled plasma (ICP). Not to be confused the older generation spark station software (I believe it was Leica Cambridge). Or the grinding wheel test that looks at the color of the spark, and the lengths of the fingers on the generated spark to estimate manganese and carbon contents.
For more information, contact me offline, as we do quite a bit of these bulk analysis metals.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dainis-at-red5wood.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: Dear Microscopists Does anyone have a spare (or is prepared to copy) manual for the Leica 1400 microtome please? Also has anyone used ImageJ with the CRI RGB Micro Color filter? Thanks Dainis
We need to dispose our working 1979 vintage JEOL 200CX to make way for a new instrument. The microscope will be taken out of service by of February 2007. The complete instrument or parts will be available to anyone who is interested. We have the manuals, service diagrams, a variety of holders as well as a few spare parts for this instrument. Please contact me offline if you are interested.
Thanks in advance.
Mohamed
************************************ Mohamed Jaffer Electron Microscope Unit University of Cape Town Private Bag Rondebosch, 7701 South Africa
Tel: +27 21 6503354 Fax: + 27 21 6891528
Email: mjaffer-at-science.uct.ac.za
************************************
==============================Original Headers============================== 13, 20 -- From mjaffer-at-science.uct.ac.za Fri Jan 19 01:21:29 2007 13, 20 -- Received: from mail.uct.ac.za (mail.uct.ac.za [137.158.128.3]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0J7LSSJ025726 13, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Jan 2007 01:21:29 -0600 13, 20 -- Received: from [137.158.37.13] (helo=science.uct.ac.za) 13, 20 -- by mail.uct.ac.za with esmtp (Exim 4.44 (FreeBSD)) 13, 20 -- id 1H7o3z-000ANv-VP 13, 20 -- for Microscopy-at-microscopy.com; Fri, 19 Jan 2007 09:21:28 +0200 13, 20 -- Message-ID: {45B07177.A37A6686-at-science.uct.ac.za} 13, 20 -- Date: Fri, 19 Jan 2007 09:21:27 +0200 13, 20 -- From: Mohamed A Jaffer {mjaffer-at-science.uct.ac.za} 13, 20 -- Reply-To: mjaffer-at-science.uct.ac.za 13, 20 -- Organization: University of Cape Town 13, 20 -- X-Mailer: Mozilla 4.75 [en] (Windows NT 5.0; U) 13, 20 -- X-Accept-Language: en 13, 20 -- MIME-Version: 1.0 13, 20 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 13, 20 -- Subject: Disposal of JEOL 200CX 13, 20 -- Content-Type: text/plain; charset=us-ascii 13, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: nmedvitz-at-bostwicklaboratories.com Name: Neil Medvitz
Organization: Bostwick Laboratories
Title-Subject: [Filtered] TEM job opening
Question: I am looking to hire a couple people to do EM in a renal pathology lab in the Richmond Virginia area. Please contact mr via e-mail if interested.
Neil Medvitz Electron Microscopist Bostwick Laboratoriesô For Absolute ConfidenceÆ
4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 x 1488 Toll Free: (800) 214-6628 Email: nmedvitz-at-bostwicklaboratories.com
-----Original Message----- X-from: dljones-at-bestweb.net [mailto:dljones-at-bestweb.net] Sent: Thursday, January 18, 2007 5:05 PM To: Fred Schamber
Andrew,
Actually, the grinding wheel test can tell a lot more than one would think. You can tell stainless steels, high speed steels, if the alloy contains cobalt, and more. I used to have an old text that listed the spark patterns for a wide range of alloys. But I haven't been able to find it in years. It was handed out in a technical course on welding I took many years ago at a community college. This was an excellent reference that had several pages of spark patterns in color identifying the various alloys that each spark test indicated. It has to be in color as color is
one of the identifiers. If anyone knows of a reference that has an extensive color chart like this, can you please send me the reference? I've been looking for a long time to find this again.
Randy,
You didn't say what the specific test was you have found to be it. Is it
indeed OES?
dj
On Thu, 18 Jan 2007, werner-at-rosharon.oilfield.slb.com wrote:
} Date: Thu, 18 Jan 2007 13:59:42 -0600 } From: werner-at-rosharon.oilfield.slb.com } To: dljones-at-bestweb.net } Subject: [Microscopy] Spark emission test? } } } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } dj is right, when metallurgists say "spark test" they generally mean } optical emission spectroscopy (OES). OES can give reliable sulfur and } phosphorous (for instance) readings at low concentrations I would never } have the chutzpa to report from EDS. } } The grinding wheel test can distinguish high-carbon (short sparks, in dense } clusters) from low-carbon steels (few sparks, long, and not in } clusters). I do not think it can distinguish whether any other alloying } elements are present or not. There is a great ASTM document - STP 550 } "Nondestructive Rapid Identification of Metals and Alloys by Spot Test" } from 1974 that covers chemical spot tests for qualitative alloy i.d. but } that is 'way off-topic. } } Regards, } Andrew } } At 11:16 AM 1/18/2007, you wrote: } } } } } ------------------------------------------------------------------------ ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ------------------------------------------------------------------------ ---- } } } } Randy, } } } } Your question of "spark emission test" sounds familiar but I can't put my } } finger on it. I can think of three techniques that may be what is being } } asked: Spark Testing, Spark Source Mass Spectrometery, or Optical Emission } } Spectroscopy. } } } } Spark Testing - this is a technique that simply uses a grinder. A piece of } } metal is pushed against the grinder. The sparks that fly off are } } indicative of what the alloy is. I only know of it being used in metal } } alloys. It would be the kind of test someone dealing with a scrap yard } } would find useful. It can basically tell you what grade of metal you are } } dealing with. It takes a fair amount of practice to be able to visually } } know what alloy the spark pattern is indicating. People that do this test } } frequently can do quite well with it as far as getting a good handle on } } what alloy they have in their hand. } } } } Spark Source Mass Spectrometery - This is used for a wide range of } } samples, but tends to do better with samples that are conductive, although } } that is not necessarily a limitation. There are two other techniques that } } are related to this: Laser Ionization Mass Spectrometery and Inductively } } Coupled Plasma Atomic Emission. } } } } Optical Emission Spectroscopy - I'm not too familiar with this technique, } } but I seem to recall they will often use a spark source in the process. } } When they do that, I think this is then called Spark Emission } } Spectroscopy. You might want to check out Bruker AXS Microanalysis, I } } believe they make some of these instruments. } } } } Perhaps if you post what they are testing and what they want from the } } analysis, that may help. I hope I've given you at least some food for } } thought. } } } } Good luck, } } } } dj } } } } } } } } On Thu, 18 Jan 2007, TindallR-at-missouri.edu wrote: } } } } } } } } } } } } } } } } ------------------------------------------------------------------------ ---- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } ------------------------------------------------------------------------ ---- } } } } } } And along a completely different line, we've been asked if we can do a } } } "spark emission test". Since I don't know what that is, and Google } } } hasn't helped, I don't know if we can or not. } } } } } } Has anybody heard of this test? Seems to be a way of checking sample } } } composition at concentrations below those attainable by EDS, but that's } } } about all I can tell. } } } } } } Sorry if this isn't microscopy-related, but I don't even know that, at } } } this point. We'll see what the collective comes up with on this one! } } } } } } Thanks to all. } } } } } } Randy } } } } } } Randy Tindall } } } Senior EM Specialist } } } Electron Microscopy Core Facility---We Do Small Well! } } } W125 Veterinary Medicine } } } University of Missouri } } } Columbia, MO 65211 } } } Tel: (573) 882-8304 } } } Fax: (573) 884-2227 } } } Email: tindallr-at-missouri.edu } } } Web: http://www.emc.missouri.edu } } } On-line calendar: } } } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } } } Week&NavType=Both&Type=TimePlan } } } } } } } } } ==============================Original } } Headers============================== } } } 7, 23 -- From TindallR-at-missouri.edu Thu Jan 18 10:12:19 2007 } } } 7, 23 -- Received: from um-tsmtpout1.um.umsystem.edu } } (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) } } } 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id l0IGCJAl020222 } } } 7, 23 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 } } 10:12:19 -0600 } } } 7, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) } } by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); } } } 7, 23 -- Thu, 18 Jan 2007 10:12:19 -0600 } } } 7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } } } 7, 23 -- Content-class: urn:content-classes:message } } } 7, 23 -- MIME-Version: 1.0 } } } 7, 23 -- Content-Type: text/plain; } } } 7, 23 -- charset="us-ascii" } } } 7, 23 -- Subject: Spark emission test? } } } 7, 23 -- Date: Thu, 18 Jan 2007 10:12:18 -0600 } } } 7, 23 -- Message-ID: } } {91108EF9255B394CBF8B7E3789814A41C68D0E-at-UM-XMAIL08.um.umsystem.edu} } } } 7, 23 -- X-MS-Has-Attach: } } } 7, 23 -- X-MS-TNEF-Correlator: } } } 7, 23 -- Thread-Topic: Spark emission test? } } } 7, 23 -- Thread-Index: Acc7G207OlT1I53OTyafRC/FuYiYDg== } } } 7, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } } } 7, 23 -- To: {microscopy-at-microscopy.com} } } } 7, 23 -- X-OriginalArrivalTime: 18 Jan 2007 16:12:19.0651 (UTC) } } FILETIME=[6DDA0930:01C73B1B] } } } 7, 23 -- Content-Transfer-Encoding: 8bit } } } 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id l0IGCJAl020222 } } } ==============================End of - } } Headers============================== } } } } } } } } } ==============================Original Headers============================== } } 13, 19 -- From dljones-at-bestweb.net Thu Jan 18 11:15:31 2007 } } 13, 19 -- Received: from vms044pub.verizon.net (vms044pub.verizon.net } } [206.46.252.44]) } } 13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id l0IHFULY021977 } } 13, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 11:15:31 } } -0600 } } 13, 19 -- Received: from localhost ([71.247.249.221]) } } 13, 19 -- by vms044.mailsrvcs.net (Sun Java System Messaging Server } } 6.2-6.01 (built Apr } } 13, 19 -- 3 2006)) with ESMTPA id {0JC200NM7QL94A26-at-vms044.mailsrvcs.net} for } } 13, 19 -- Microscopy-at-microscopy.com; Thu, 18 Jan 2007 11:15:14 -0600 (CST) } } 13, 19 -- Date: Thu, 18 Jan 2007 12:14:16 -0500 (Eastern Standard Time) } } 13, 19 -- From: "David L. Jones" {dljones-at-bestweb.net} } } 13, 19 -- Subject: Re: [Microscopy] Spark emission test? } } 13, 19 -- In-reply-to: {200701181618.l0IGIW0e026577-at-ns.microscopy.com} } } 13, 19 -- X-X-Sender: dljones-at-pop-croton.bestweb.net } } 13, 19 -- To: TindallR-at-missouri.edu } } 13, 19 -- Cc: Microscopy-at-microscopy.com } } 13, 19 -- Message-id: {Pine.WNT.4.64.0701181137340.3320-at-H-F1} } } 13, 19 -- MIME-version: 1.0 } } 13, 19 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed } } 13, 19 -- References: {200701181618.l0IGIW0e026577-at-ns.microscopy.com} } } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 8, 36 -- From werner-at-rosharon.oilfield.slb.com Thu Jan 18 13:55:02 2007 } 8, 36 -- Received: from mail.slb.com (nammta01.sugar-land.nam.slb.com [163.188.150.130]) } 8, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0IJt2Wl003343 } 8, 36 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 13:55:02 -0600 } 8, 36 -- Received: from pmxchannel_int-daemon.nammta01.sugar-land.nam.slb.com by } 8, 36 -- nammta01.sugar-land.nam.slb.com } 8, 36 -- (iPlanet Messaging Server 5.2 Patch 2 (built Jul 14 2004)) } 8, 36 -- id {0JC200301XENHX-at-nammta01.sugar-land.nam.slb.com} for } 8, 36 -- microscopy-at-microscopy.com; Thu, 18 Jan 2007 19:42:23 +0000 (GMT) } 8, 36 -- Received: from usxsl052.slb.atosorigin-asp.com } 8, 36 -- (usxsl052.slb.atosorigin-asp.com [199.6.136.167]) } 8, 36 -- by nammta01.sugar-land.nam.slb.com } 8, 36 -- (iPlanet Messaging Server 5.2 Patch 2 (built Jul 14 2004)) } 8, 36 -- with ESMTP id {0JC200G99XEN8P-at-nammta01.sugar-land.nam.slb.com} for } 8, 36 -- microscopy-at-microscopy.com; Thu, 18 Jan 2007 19:42:23 +0000 (GMT) } 8, 36 -- Received: from pmxchannel-daemon.us085mbx01.slb.atosorigin-asp.com by } 8, 36 -- us085mbx01.slb.atosorigin-asp.com } 8, 36 -- (Sun Java System Messaging Server 6.2-1 (built Feb 24 2005)) } 8, 36 -- id {0JC20092VXEJW500-at-us085mbx01.slb.atosorigin-asp.com} for } 8, 36 -- microscopy-at-microscopy.com; Thu, 18 Jan 2007 19:42:19 +0000 (GMT) } 8, 36 -- Received: from WERNER1-OFS.rosharon.oilfield.slb.com ([163.188.46.5]) } 8, 36 -- by us085mbx01.slb.atosorigin-asp.com } 8, 36 -- (Sun Java System Messaging Server 6.2-1 (built Feb 24 2005)) } 8, 36 -- with ESMTPSA id {0JC2003Z9XEJQX50-at-us085mbx01.slb.atosorigin-asp.com} for } 8, 36 -- microscopy-at-microscopy.com; Thu, 18 Jan 2007 19:42:19 +0000 (GMT) } 8, 36 -- Date: Thu, 18 Jan 2007 13:42:18 -0600 } 8, 36 -- From: Andrew Werner {werner-at-rosharon.oilfield.slb.com} } 8, 36 -- Subject: Re: [Microscopy] Re: Spark emission test? } 8, 36 -- In-reply-to: {200701181716.l0IHGH9F023234-at-ns.microscopy.com} } 8, 36 -- To: microscopy-at-microscopy.com } 8, 36 -- Message-id: {6.2.1.2.2.20070118133232.03439d28-at-us1061-pop3.mail.slb.com} } 8, 36 -- MIME-version: 1.0 } 8, 36 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2 } 8, 36 -- Content-type: text/plain; charset=us-ascii; format=flowed } 8, 36 -- Content-transfer-encoding: 7BIT } 8, 36 -- References: {200701181716.l0IHGH9F023234-at-ns.microscopy.com} } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 20 -- From "dljones-at-bestweb.net"-at-bestweb.net Thu Jan 18 15:57:08 2007 9, 20 -- Received: from mta5.srv.hcvlny.cv.net (mta5.srv.hcvlny.cv.net [167.206.4.200]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0ILv7Cf016738 9, 20 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jan 2007 15:57:08 -0600 9, 20 -- Received: from localhost (ool-44c62883.dyn.optonline.net [68.198.40.131]) 9, 20 -- by mta5.srv.hcvlny.cv.net 9, 20 -- (Sun Java System Messaging Server 6.2-6.01 (built Apr 3 2006)) 9, 20 -- with ESMTP id {0JC300E9X3MIVOF0-at-mta5.srv.hcvlny.cv.net} for 9, 20 -- microscopy-at-microscopy.com; Thu, 18 Jan 2007 16:56:59 -0500 (EST) 9, 20 -- Date: Thu, 18 Jan 2007 16:48:42 -0500 (Eastern Standard Time) 9, 20 -- From: "David L. Jones" {"dljones-at-bestweb.net"-at-bestweb.net} 9, 20 -- Subject: Re: [Microscopy] Spark emission test? 9, 20 -- In-reply-to: {200701181959.l0IJxg9t010372-at-ns.microscopy.com} 9, 20 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 9, 20 -- Cc: microscopy-at-microscopy.com 9, 20 -- Message-id: {Pine.WNT.4.64.0701181516340.176-at-dljtoshiba} 9, 20 -- MIME-version: 1.0 9, 20 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 9, 20 -- Content-transfer-encoding: 7BIT 9, 20 -- References: {200701181959.l0IJxg9t010372-at-ns.microscopy.com} ==============================End of - Headers==============================
==============================Original Headers============================== 19, 20 -- From fschamber-at-aspexcorp.com Fri Jan 19 10:36:01 2007 19, 20 -- Received: from aspexcorp2k6.local (mail.aspexcorp.com [67.141.199.81]) 19, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0JGa0R0025169 19, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Jan 2007 10:36:00 -0600 19, 20 -- Content-class: urn:content-classes:message 19, 20 -- MIME-Version: 1.0 19, 20 -- Content-Type: text/plain; 19, 20 -- charset="us-ascii" 19, 20 -- Subject: RE: [Microscopy] Re: Spark emission test? 19, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 20 -- Date: Fri, 19 Jan 2007 11:35:50 -0500 19, 20 -- Message-ID: {65657329C810FE4B96B17390F9BE463E06BF3E-at-ASPEXCORP2K3.aspexcorp2k6.local} 19, 20 -- X-MS-Has-Attach: 19, 20 -- X-MS-TNEF-Correlator: 19, 20 -- Thread-Topic: [Microscopy] Re: Spark emission test? 19, 20 -- Thread-Index: Acc7TLT9vKeUQ6lMRFO7a5jCaV9I6QAmY4Pg 19, 20 -- From: "Fred Schamber" {fschamber-at-aspexcorp.com} 19, 20 -- To: {Microscopy-at-microscopy.com} 19, 20 -- Content-Transfer-Encoding: 8bit 19, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0JGa0R0025169 ==============================End of - Headers==============================
Kurt: Since my company banned cyanide-based etches some years back. one of my favorite gold etches is as follows:
4.6 grams potassium iodide 1.3 grams Iodine 100 milliliters of DI water (ratios can be increased/decreased as needed)
Use at room temp. This removes 2-4 microns of Au in around 5 minutes, according to my notes. You might want to test it on something expendable before you use it on the real deal. It will store a long time.
klangwor-at-uoregon.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both klangwor-at-uoregon.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: klangwor-at-uoregon.edu } Name: Kurt Langworthy } } Organization: University of Oregon } } Title-Subject: [Filtered] EBL/ PMMA Mask } } Question: All, } I'm looking for a gold etching recipt that will allow me to use PMMA as a mask. KCN looks promising, but is highly toxic. Does anyone have any better ideas? } Thanks, } Kurt } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 12 -- From zaluzec-at-microscopy.com Wed Jan 17 18:34:22 2007 } 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0I0YJlb010881 } 6, 12 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jan 2007 18:34:21 -0600 } 6, 12 -- Mime-Version: 1.0 } 6, 12 -- X-Sender: (Unverified) } 6, 12 -- Message-Id: {p06110407c1d470f44a74-at-[206.69.208.22]} } 6, 12 -- Date: Wed, 17 Jan 2007 18:34:19 -0600 } 6, 12 -- To: microscopy-at-microscopy.com } 6, 12 -- From: klangwor-at-uoregon.edu (by way of MicroscopyListserver) } 6, 12 -- Subject: viaWWW: EBL/ PMMA Mask } 6, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== } }
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 6, 23 -- From r-holdford-at-ti.com Fri Jan 19 17:40:58 2007 6, 23 -- Received: from calf.ext.ti.com (calf.ext.ti.com [198.47.26.144]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0JNew1s012201 6, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 19 Jan 2007 17:40:58 -0600 6, 23 -- Received: from dlep33.itg.ti.com ([157.170.170.112]) 6, 23 -- by calf.ext.ti.com (8.13.7/8.13.7) with ESMTP id l0JNeRND006255 6, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 6, 23 -- Fri, 19 Jan 2007 17:40:38 -0600 6, 23 -- Received: from [156.117.194.120] (localhost [127.0.0.1]) 6, 23 -- by dlep33.itg.ti.com (8.13.7/8.13.7) with ESMTP id l0JNeMol019754; 6, 23 -- Fri, 19 Jan 2007 17:40:22 -0600 (CST) 6, 23 -- Message-ID: {45B156E5.4050106-at-ti.com} 6, 23 -- Date: Fri, 19 Jan 2007 17:40:21 -0600 6, 23 -- From: Becky Holdford {r-holdford-at-ti.com} 6, 23 -- Organization: SC Packaging Development -- FA Development 6, 23 -- User-Agent: Thunderbird 1.5.0.9 (Windows/20061207) 6, 23 -- MIME-Version: 1.0 6, 23 -- To: klangwor-at-uoregon.edu, MSA ListServer {Microscopy-at-MSA.Microscopy.Com} 6, 23 -- Subject: Re: [Microscopy] viaWWW: EBL/ PMMA Mask 6, 23 -- References: {200701180034.l0I0YWPP011425-at-ns.microscopy.com} 6, 23 -- In-Reply-To: {200701180034.l0I0YWPP011425-at-ns.microscopy.com} 6, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jeremy.wilburn-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jeremy.wilburn-at-gmail.com Name: Jeremy Wilburn
Question: I am a chemistry grad student working with both live cells and cultured pancreatic islets. More specifically I do electrochemical (electrophysiology) measurements with ultramicroelectrodes, and am needing to put together an instrument similar to a patch-clamp on an inverted microscope. I am in the market for an inverted microscope now, but am limited in funding (~$8-10k).
My problem is the following. Since I use a capillary electrode body (which must remain completely vertical, unlike a patch pipette), I am unable to use the top condenser for imaging (due to both space restraints and shadowing from the electrode body). I was thinking to get a fluorescence-type inverted microscope and remove the filter, making a bottom-illumination similar to a metallurgical microscope, but would still let me do fluorescence at some point...would this work?
My second option would be a more expensive scope (like Olympus IX71) that has multiple light ports...
Any other suggestions (including a good scope to look for) would be greatly appreciated.
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Organization: EMAT, University of Antwerp, Belgium
Title-Subject: [Filtered] Electropolishing TiNiAu
Question: Dear microscopists,
I am currently looking for a suitable electropolishing solution for the ternary shape-memory alloys TiNiAu in order to have a thinned sample for electron microscopy.
I already tried several solutions that are known to work well with TiNi. Unfortunately, the added gold at a concentration around 15 at% seems to severely hamper the electro-polishing: the same conditions as for Ni-Ti give etched specimens with bad edges of the hole. We've played with tension, temperature, fluid speed,etc.. but without success.
Solutions tried that work with NiTi but not with my sample:
I already looked up in the literature and couldn't find anything close to my alloy. So I was wondering if you might have a suggestion of an alternative, eg, adding an extra acid to one of the above baths or maybe something completely different.
Many thanks in advance,
RÈmi Delville
EMAT - Electron Microscopy for Material Science - University of Antwerp, Belgium
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Email: peter.tomic-at-renwireless.com Name: Peter Tomic
Organization: Renaissance Wireless
Title-Subject: [Filtered] FEI XL-50 Experience
Question: Listers,
I'd like to hear from anyone that has owned/operated an FEI XL50 SEM. I have one we are bringing up in our MEMS lab. There are some unusual aspects to this instrument such as the exchange port.
My understanding is that there were not very many XL-50's made. Does anyone currently own one?
You may respond on-list or off-list if you care to.
Peter Tomic Renaissance Wireless Corp. Somerset, New Jersey, USA
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Email: liang-at-saturn.med.nyu.edu Name: Alice Liang
Organization: NYU school of Medicine
Title-Subject: [Filtered] Research Technician in EM core facility
Question: Research Technician ñ Electron Microscopy The Image Core Facility of Skirball Institute, NYU School of Medicine
A full - time research technician position is currently available in the Image Core Facility at Skirball Institute of NYU School of Medicine. The facility provides essential electron microscopic and structural analysis support for school of medicine and local community. The successful candidate must have at least a B.S. degree, appropriate training and significant biomedical research experience in histology, electron microscopy (TEM) and tissue sample preparation. Candidates with an excellent communication skill and experience in instrument maintenance are encouraged to apply. Skirball is an academic organization and offers competitive salaries and excellent fringe benefits. Interested individuals should send resume and three references to:
Alice Liang, Ph.D. Director of Image Core Facility Skirball Institute of Biomolecular Medicine Skirball 2nd floor, EM Suite NYU School of Medicine New York, NY 10016 Tel: 212-263-7644 (o); 212-263-7099 (Lab) Fax: 212-263-7643 E-mail: liang-at-saturn.med.nyu.edu http://saturn.med.nyu.edu/facilities/imagecore/
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Title-Subject: [Filtered] Positions available: Technical University of Denmark
Question: Immediate opening:
----------------------------------------------------------------- Senior scientists in Electron Microscopy at the Center for Electron Nanoscopy, Technical University of Denmark
Apply before: 12.02.2007 at 12:00 (noon)
The Center for Electron Nanoscopy (CEN) at the Technical University of Denmark (DTU), which has been made possible by a generous donation from the A.P. Moller Foundation, seeks to appoint one or more senior scientists to permanent positions in advanced electron microscopy for materials science and nanotechnology. The number of appointments will depend on the quality and experience of the applicants.
CEN-DTU will be fully operational in 2007 and will contain seven new electron microscopes. There will be two aberration-corrected monochromated TEMs, one of which will be equipped with a gas reaction capability.
Applicants must have outstanding international research reputations and hands-on expertise in a wide range of advanced electron microscopy techniques. They will be expected to perform experimental work with users of the facility, and to assist CEN's director in raising research funding and in developing an internationally leading program of advanced research.
Expertise in one or more of the following areas is essential: gas reaction TEM; aberration-correction; monochromated EELS; electron tomography; electron holography; direct methods; advanced image analysis and simulation for quantitative electron microscopy.
Positions are to be filled in 2007, on dates to be agreed with the successful applicants.
The salary and appointment terms will be based on the current collective agreement for Danish University faculty members.
All interested candidates, irrespective of age, gender, race, religion and ethnic background, are invited to apply.
Further information can be obtained from CEN's director Rafal Dunin-Borkowski (rafaldb-at-gmail.com).
Applications, which should include a resumÈ, list of publications and statement of research interests, should be sent to
The Rector Technical University of Denmark Building 101 A DK-2800 Lyngby, Denmark
and must be received before 12:00 on 12 February 2007.
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This message is in response to the thread about Polaron E5100 sputter coaters and operating conditions: HV knob setting and voltages to be used. I saw mention that finer grain size of the sputtered film is achieved when a lower voltage (I think 700V was mentioned) is used, and I have seen this elsewhere. I'm trying to come to terms with this and sometimes it is worth going back to basics.
Several sources show graphs of the Voltage and Current relationships in a DC plasma:
http://science-education.pppl.gov/SummerInst/SGershman/Structure_of_Glow_Discharge.pdf (Sophia Gershman must be one heck of a High School teacher!)
These data show that for the range of conditions typically used in a sputter coater the actual voltage that exists in the plasma is clamped by the physics of the plasma and while the knob may set 700V or 2.2kV, the difference between the set voltage and the voltage across the plasma must be accounted for by IR drop in the winding resistance and any ballasting/limiting resistors in the circuit (the E5100 has resistance in the primary leads, 5k ohm in the secondary lead, plus the considerable winding resistance of the secondary HV winding). The description of the behaviour of the plasma is that the current increases by increasing the volume of the plasma - any you can see this for yourself - but as best I can judge by the graphs in the referenced papers, the voltage may increase only very slightly, and it is difficult for me to see that it would change the energy of electrons bombarding the target significantly.
Is the "finer grain with lower voltage" real? If it is real, what is the basis for it? Have I completely misinterpreted the data?
The reflected light imaging will work, but your biggest issue will be contrast in the biological samples. And for reflected light imaging you do NOT need or want a high brightness light source like those used for florescence work. Cost savings ($100 vs $5000).
As for contrast reflected light DIC might be ideal however since it has depth of focus issues seeing the electrode as it approaches the cell would be problematic. And it is costly. I suggest you look at reflected light dark-field. A rare beast but might be what you need.
Another approach would be oblique illumination. I seem to remember that some inverted scopes had/have tilting lamp/condensors so you can tilt them off axis 35? 45? 60-degrees or so. . . . in fact I just went and checked my IX-81 illumination column tilts backwards, and the manual states "Even with the illumination column tilted the specimen surface will be illuminated, which is convient for rough confirmation of the specimen location on initial positioning of the specimen." My guess would be that setting Köhler illumination for highest resolution would be an issue but would still allow very reasonable bright-field imaging.
good luck.
On 20 Jan 2007 at 9:29, jeremy.wilburn-at-gmail.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both jeremy.wilburn-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: jeremy.wilburn-at-gmail.com } Name: Jeremy Wilburn } } Organization: Vanderbilt University } } Title-Subject: [Filtered] biological microscopy question } } Question: I am a chemistry grad student working with both live cells and cultured pancreatic islets. More specifically I do electrochemical (electrophysiology) measurements with ultramicroelectrodes, and am needing to put together an instrument similar to a patch-clamp on an inverted microscope. I am in the market for an inverted microscope now, but am limited in funding (~$8-10k). } } My problem is the following. Since I use a capillary electrode body (which must remain completely vertical, unlike a patch pipette), I am unable to use the top condenser for imaging (due to both space restraints and shadowing from the electrode body). I was thinking to get a fluorescence-type inverted microscope and remove the filter, making a bottom-illumination similar to a metallurgical microscope, but would still let me do fluorescence at some point...would this work? } } My second option would be a more expensive scope (like Olympus IX71) that has multiple light ports... } } Any other suggestions (including a good scope to look for) would be greatly appreciated. } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 12 -- From zaluzec-at-microscopy.com Sat Jan 20 09:27:37 2007 } 9, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0KFRbth004257 } 9, 12 -- for {microscopy-at-microscopy.com} ; Sat, 20 Jan 2007 09:27:37 -0600 } 9, 12 -- Mime-Version: 1.0 } 9, 12 -- X-Sender: (Unverified) } 9, 12 -- Message-Id: {p06110404c1d7e559607a-at-[206.69.208.22]} } 9, 12 -- Date: Sat, 20 Jan 2007 09:27:35 -0600 } 9, 12 -- To: microscopy-at-microscopy.com } 9, 12 -- From: jeremy.wilburn-at-gmail.com (by way of MicroscopyListserver) } 9, 12 -- Subject: viaWWW: biological microscopy question } 9, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"A main-frame: The biggest PC peripheral available."
==============================Original Headers============================== 15, 33 -- From edelmare-at-muohio.edu Sat Jan 20 14:54:54 2007 15, 33 -- Received: from spamfirewall.muohio.edu (walrus.mcs.muohio.edu [134.53.6.27]) 15, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0KKsrKX009982 15, 33 -- for {microscopy-at-Microscopy.com} ; Sat, 20 Jan 2007 14:54:53 -0600 15, 33 -- X-ASG-Debug-ID: 1169326488-3fd400980000-Dem1zR 15, 33 -- X-Barracuda-URL: http://spamfirewall.muohio.edu:80/cgi-bin/mark.cgi 15, 33 -- X-Barracuda-Connect: mulnx24.mcs.muohio.edu[134.53.6.11] 15, 33 -- X-Barracuda-Start-Time: 1169326488 15, 33 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 15, 33 -- by spamfirewall.muohio.edu (Spam Firewall) with ESMTP 15, 33 -- id A4F9033C62D; Sat, 20 Jan 2007 15:54:48 -0500 (EST) 15, 33 -- Received: from [192.168.1.23] ([134.53.14.105]) 15, 33 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l0KKsmU4020217; 15, 33 -- Sat, 20 Jan 2007 15:54:48 -0500 15, 33 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 15, 33 -- To: jeremy.wilburn-at-gmail.com 15, 33 -- Date: Sat, 20 Jan 2007 15:54:46 -0500 15, 33 -- MIME-Version: 1.0 15, 33 -- X-ASG-Orig-Subj: Re: [Microscopy] viaWWW: biological microscopy question 15, 33 -- Subject: Re: [Microscopy] viaWWW: biological microscopy question 15, 33 -- CC: microscopy-at-Microscopy.com 15, 33 -- Message-ID: {45B23B46.5131.11055C7D-at-edelmare.muohio.edu} 15, 33 -- Priority: normal 15, 33 -- In-reply-to: {200701201529.l0KFTDqB008077-at-ns.microscopy.com} 15, 33 -- References: {200701201529.l0KFTDqB008077-at-ns.microscopy.com} 15, 33 -- X-mailer: Pegasus Mail for Windows (4.41) 15, 33 -- Content-type: text/plain; charset=ISO-8859-1 15, 33 -- Content-description: Mail message body 15, 33 -- X-Barracuda-Virus-Scanned: by Barracuda Spam Firewall at muohio.edu 15, 33 -- X-Barracuda-Spam-Score: 0.00 15, 33 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=3.5 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=1000.0 15, 33 -- Content-Transfer-Encoding: 8bit 15, 33 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id l0KKsrKX009982 ==============================End of - Headers==============================
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Email: mgb-at-ansto.gov.au Name: Mark Blackford
Organization: Australian Nuclear Science and Technology Organisation
Title-Subject: [Filtered] Wanted: anode chamber for JEOL 2000fxII TEM
Question: Hi All,
I'm hoping someone may be able to help locate a working anode chamber (electron gun) for a JEOL 2000fxII TEM. Our microscope can only be operated at 100kV due to electrical discharging in the anode chamber at higher accelerating voltages. Several attempts at cleaning the internal components have not been entirely successful.
A fully refurbished unit can be supplied by JEOL, but not cheaply.
I would like to contact anyone who has a suitable fully functional anode chamber, capable of working at 200kV, that they don't happen to need anymore. Please contact me off-line. Cheers,
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Email: dmcdaniel-at-usuhs.mil Name: Dennis McDaniel
Title-Subject: [Filtered] LR White polymerization problem
Question: I am having difficulty polymerizing a sample in LR White. The cells (macropahges and dendritic cells) were grown on a piece of aclar, fixed with formaldehyde/glutaraldehyde, post-fixed with uranyl acetate, dehydrated in a graduated series of EtOH, and infiltrated with LR White. I then sandwiched the aclar film containing the cells between two larger pieces of aclar (since the edges don't polymerize due to air exposure) and put the sample in the oven at 55C. After 48h, the resin immediately around the piece of aclar containing the cells appears very fragmented and cracked and is obviously unsuitable for sectioning, while the resin further from the cells appears to have polymerized properly. At first I thought it was an infiltration problem, but I did several changes of 100% resin and even left the samples overnight on a rotator at room temperature in 100% resin to ensure complete infiltration. Does anyone have any idea what they problem might be? Thanks.
Dennis, I think that your problem is a result of incomplete dehydration. LR White is supposed to handle some water without problem, but when you sandwich the pieces, you create a limited volume for diffusion and the water becomes too great locally. Make sure your ethanol is dry (on molecular sieve or freshly opened). That's my theory. Kim
dmcdaniel-at-usuhs.mil wrote:
} Email: dmcdaniel-at-usuhs.mil } Name: Dennis McDaniel } } Title-Subject: [Filtered] LR White polymerization problem } } Question: I am having difficulty polymerizing a sample in LR White. The cells (macropahges and dendritic cells) were grown on a piece of aclar, fixed with formaldehyde/glutaraldehyde, post-fixed with uranyl acetate, dehydrated in a graduated series of EtOH, and infiltrated with LR White. I then sandwiched the aclar film containing the cells between two larger pieces of aclar (since the edges don't polymerize due to air exposure) and put the sample in the oven at 55C. After 48h, the resin immediately around the piece of aclar containing the cells appears very fragmented and cracked and is obviously unsuitable for sectioning, while the resin further from the cells appears to have polymerized properly. At first I thought it was an infiltration problem, but I did several changes of 100% resin and even left the samples overnight on a rotator at room temperature in 100% resin to ensure complete infiltration. Does anyone have any idea what they problem might be? Thanks. } } ---------------------------------------------------------------------------
-- Kim Rensing Ph.D. Manager, Microscopy and Imaging Facility
University of Calgary, Health Sciences Centre B129 - 3330 Hospital Drive NW Calgary, AB, Canada T2N 4N1 403-220-3488 krensing-at-ucalgary.ca
==============================Original Headers============================== 6, 22 -- From krensing-at-ucalgary.ca Mon Jan 22 09:55:23 2007 6, 22 -- Received: from smtp2.ucalgary.ca (smtp2.ucalgary.ca [136.159.34.223]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0MFtNkD018643 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 22 Jan 2007 09:55:23 -0600 6, 22 -- Received: from [136.159.164.163] (unknown [136.159.164.163]) 6, 22 -- by smtp2.ucalgary.ca (Postfix) with ESMTP id 501331004B; 6, 22 -- Mon, 22 Jan 2007 08:55:22 -0700 (MST) 6, 22 -- Message-ID: {45B4DE64.1000509-at-ucalgary.ca} 6, 22 -- Date: Mon, 22 Jan 2007 08:55:16 -0700 6, 22 -- From: Kim Rensing {krensing-at-ucalgary.ca} 6, 22 -- Organization: Microscopy and Imaging Facility, U. of Calgary 6, 22 -- User-Agent: Thunderbird 1.5.0.9 (Windows/20061207) 6, 22 -- MIME-Version: 1.0 6, 22 -- To: dmcdaniel-at-usuhs.mil, microscopy-at-microscopy.com 6, 22 -- Subject: Re: [Microscopy] viaWWW: LR White polymerization problem 6, 22 -- References: {200701221414.l0MEEtPY012668-at-ns.microscopy.com} 6, 22 -- In-Reply-To: {200701221414.l0MEEtPY012668-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-UCalgary-MailScanner-Information: Please contact IT Help Desk at (403) 220-5555 for more information 6, 22 -- X-UCalgary-MailScanner: Found to be clean 6, 22 -- X-UCalgary-MailScanner-From: krensing-at-ucalgary.ca ==============================End of - Headers==============================
I suspect that your polymerization problems arise from entrapped air. I eliminate that problem by purging my oven with nitrogen, thus alleviating other more tedious approaches. Works every time.
Regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
dmcdaniel-at-usuh s.mil To gary.m.brown-at-exxonmobil.com 01/22/07 08:08 cc AM Subject [Microscopy] viaWWW: LR White Please respond polymerization problem to dmcdaniel-at-usuh s.mil
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Email: dmcdaniel-at-usuhs.mil Name: Dennis McDaniel
Title-Subject: [Filtered] LR White polymerization problem
Question: I am having difficulty polymerizing a sample in LR White. The cells (macropahges and dendritic cells) were grown on a piece of aclar, fixed with formaldehyde/glutaraldehyde, post-fixed with uranyl acetate, dehydrated in a graduated series of EtOH, and infiltrated with LR White. I then sandwiched the aclar film containing the cells between two larger pieces of aclar (since the edges don't polymerize due to air exposure) and put the sample in the oven at 55C. After 48h, the resin immediately around the piece of aclar containing the cells appears very fragmented and cracked and is obviously unsuitable for sectioning, while the resin further from the cells appears to have polymerized properly. At first I thought it was an infiltration problem, but I did several changes of 100% resin and even left the samples overnight on a rotator at room temperature in 100% resin to ensure complete infiltration. Does anyone have any idea what they problem might be? Thanks.
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Title-Subject: [Filtered] Part for print processor
Question: Does anyone have a MohrPro8, model ME-42 print processor they are no longer using? I need to obtain the roller assembly that processes the print through the water and drying. My roller assembly is broken beyond repair and this model is no longer made. I am willing to pay for shipment.
Thanks, Patricia Zerfas National Institute of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 USA ph # (301) 496-4464
I regret to inform you of the passing of fellow electron microscopist, friend, neighbor and mentor, James Hillier. James Hillier of Princeton, NJ developed the first operational electron microscope in 1938, died Monday January 15, 2007 at University Medical Center at Princeton. He was 91. He was a senior scientist and vice president at the Radio Corporation of America and a director of RCA's David Sarnoff Research Center.
As a graduate student at the University of Toronto in 1938, under Professor Eli Franklin Burton, Mr. Hillier and fellow student Albert Prebus designed and built the first north American commercial electron microscope prototype.
After obtaining a master's degree and a doctorate in 1941 from the University of Toronto, he was immediately hired by The Radio Corporation of America in Camden, where he developed the first commercially available electron microscope, later developing RCA's videodisc, a precursor to the DVD. He rose through the corporate ranks to become executive vice president for research and engineering and senior scientist at RCA in 1969. He also served as the director of the David Sarnoff Research Center in West Windsor.
Mr. Hillier held 41 patents for devices and processes, the most significant for the electron microscope. After receiving a joint award from the American Public Health Association and the Albert and Mary Lasker Foundation for medical research in 1960, Mr. Hillier said to a reporter from Time magazine, "The electron microscope is like the monkey wrench on the garage wall; what you do with it is the important thing."
After retiring from RCA in 1977, he focused his attention on the National Inventors Hall of Fame in Akron, Ohio, and later The James Hillier Foundation for Science Education, dedicated to funding the college education of bright scientifically oriented students. In 1993, he established the James Hillier Foundation, which awards scholarships each year to science students from Brant County, Ontario.
Dr. Hillier received honorary degrees from New Jersey Institute of Technology and the University of Toronto. A public school in Brantford, Ontario, his birthplace, has been named in his honor. He was one of the first scientists elected to the National Inventors Hall of Fame in 1980. In 1997, he was made officer of the Order of Canada, the country's highest civilian honor.
His late wife owned and managed the Flower Basket in Princeton and later owned two additional Princeton flower shops. Both artists, Mr. Hillier made photo-realistic pastels and drawings while his wife was an abstract artist and an award-winning flower arranger. Son of the late James and Ethel Cooke Hillier, husband of the late Florence M. Bell Hillier, who died in 1992 after 55 years of marriage, father of the late William W. Hillier, who died in 2002, he is survived by his son, architect James Robert Hillier of New Hope, Pa.; sisters May Hillier of Brantford, Ontario, and Thelma Henshaw of Naples, Fla.; three grandsons; a granddaughter; and three great-grandchildren.
In lieu of flowers, memorial contributions may be made to the James Hillier Foundation, 34 Hill Ave., Brantford, Ontario, Canada, N3R 4HI. {http://comdir.bfree.on.ca/hillier/index.html} http://comdir.bfree.on.ca/hillier/index.html
Sincerely,
Peter P. Tarquinio
Evex Inc., Peter Tarquinio 857 State Road Princeton, NJ 08540
We are re-building and replacing the gun FE tip on an Amray 1850 FE. Question: What is the tolerance, i.e., the distance from the suppressor to the extractor? We have a sketch showing 0.765mm, but no plus or minus tolerance. Does anyone know what the tolerance should be?
Thank you for your help.
Larry Zagorski Sr. Metallurgist FAI Materials Testing Laboratory 825 Chance Road Marietta, GA 30066 770-928-1930 larryz-at-fai.us
==============================Original Headers============================== 5, 17 -- From LarryZ-at-fai.us Tue Jan 23 13:04:08 2007 5, 17 -- Received: from mx.cbeyond.com (mx.cbeyond.com [66.180.96.58]) 5, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0NJ478I032500 5, 17 -- for {Microscopy-at-microscopy.com} ; Tue, 23 Jan 2007 13:04:08 -0600 5, 17 -- Received: from [69.15.9.254] (port=3292 helo=[127.0.0.1]) 5, 17 -- by mx.cbeyond.com with asmtp (Exim 4.34) 5, 17 -- id 1H9QwB-0006SF-EU 5, 17 -- for Microscopy-at-microscopy.com; Tue, 23 Jan 2007 14:04:07 -0500 5, 17 -- Message-ID: {45B660B7.4080308-at-fai.us} 5, 17 -- Date: Tue, 23 Jan 2007 14:23:35 -0500 5, 17 -- From: Larry Zagorski {LarryZ-at-fai.us} 5, 17 -- User-Agent: Thunderbird 1.5.0.9 (Windows/20061207) 5, 17 -- MIME-Version: 1.0 5, 17 -- To: Microscopy-at-microscopy.com 5, 17 -- Subject: Rebuilding Amray 1850 FE Gun 5, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I was going through some cabinets and came across a Vigor TW-1000 tweezer sharpener (sold by Pella, apparently, a long time ago, judging by the yellowing of the box) sans instructions. I think I've figured out how the thing works, but would like a copy of the instructions to see if I have all the parts and all those parts in the correct location. Is there anyone out there in the ether who has a copy they could scan, xerox, or otherwise send to me? The beer is on me and I'll show you the fantastically high tides if you ever find yourself in Sackville, New Brunswick (doesn't everybody wish they were here?).
Thank in advance,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University Sackville, NB E4L 1G7 CANADA
Did you try contacting Ted Pella? They may be able to help.
Mannie Steglich
jehrman-at-mta.ca
01/24/2007 12:30 PM Please respond to jehrman
To: msteglic-at-mdanderson.org cc:
Greetings listers,
I was going through some cabinets and came across a Vigor TW-1000 tweezer sharpener (sold by Pella, apparently, a long time ago, judging by the yellowing of the box) sans instructions. I think I've figured out how the thing works, but would like a copy of the instructions to see if I have all the parts and all those parts in the correct location. Is there anyone out there in the ether who has a copy they could scan, xerox, or otherwise send to me? The beer is on me and I'll show you the fantastically high tides if you ever find yourself in Sackville, New Brunswick (doesn't everybody wish they were here?).
Thank in advance,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University Sackville, NB E4L 1G7 CANADA
After the usual glut of "Out of Office" replies (gotta love those people -- can't shoot 'em) I have a contact who'll send me a copy of the instructions. Thanks for the other offers.
Cheers,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University Sackville, NB E4L 1G7 CANADA
Does anyone have any pet techniques/fixatives for TEM processing of brain tissue? Specifically mouse brain, if it matters. We just did a run with less than stellar results----poor membranes, etc. We are now trying a couple different things, but if anyone has some tried-and-true tips, it might save us a ton of time. No immunolabeling, just ultrastructure.
Some recipes call for picric acid. Can anyone tell me what purpose this serves?
Thanks a heap, as usual!
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 7, 23 -- From TindallR-at-missouri.edu Wed Jan 24 13:44:05 2007 7, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0OJi4V8007057 7, 23 -- for {microscopy-at-microscopy.com} ; Wed, 24 Jan 2007 13:44:05 -0600 7, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 7, 23 -- Wed, 24 Jan 2007 13:44:04 -0600 7, 23 -- x-mimeole: Produced By Microsoft Exchange V6.5 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="us-ascii" 7, 23 -- Subject: TEM: Fixation of mouse brain tissue 7, 23 -- Date: Wed, 24 Jan 2007 13:44:04 -0600 7, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68D40-at-UM-XMAIL08.um.umsystem.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- Thread-Topic: TEM: Fixation of mouse brain tissue 7, 23 -- Thread-Index: Acc/8AJnCy/SuSXRTS2WyasNFvjxlQ== 7, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 7, 23 -- To: {microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 24 Jan 2007 19:44:04.0365 (UTC) FILETIME=[00EE0BD0:01C73FF0] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0OJi4V8007057 ==============================End of - Headers==============================
CNS tissue is best fixed by perfusion, without a doubt. Conventional procedures (excision) hardly ever give very good results with CNS.
So, my first question is: did you use perfusion?
JB
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-- ############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Email: bozzola-at-siu.edu ##############################################################
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I use 2-3% glutaraldehyde in 0.1M phosphate buffer at room temp., pH 7.3-7.4, perfused via the left ventricle. While I have a small peristaltic pump I have used gravity with excellent results. While some will argue otherwise, one does need a fancy apparatus or high pressure, I don't even measure the pressure. I use just a few ml of the same buffer (without fancy additives) as a washout and at least 50-75 ml of fix perfused in 5 minutes. In my experience most labs with less than optimal results use 1. a tiny little needle in the ventricle 2. an inadquate rate of flow and 3. way too much washout. Use an 18-20 g needle (same inside diameter as the aorta!) inserted into the vent. then have the assistant just nick the r. atrium, being careful not to go too deep and cut the aorta .......-at-#$%^!! Going too far into the vent. can be a problem so put a piece of cork or tygon tubing aroung the shaft of the needle so you don't go too deep. Make everything fresh. Fix for a few hours at room temp., multiple rinses in buffer, osmicate (reasonably fresh osmium) thin slices/small pieces in the same buffer for several hours, multiple buffer rinses, store in buffer if needed. If you must keep orientation cut 200 micron Vibratome sections (before osmication) so you can see the antomy after osmium turns everything black. Dehydrate rapidly in graded ethanols (too much time in ethanols will remove cytoplasm, Hyatt's book has the illustrations and refs. to prove this), clear, infiltrate and embed. Uranyl acetate (after multiple washes in sodium acetate to remove phosphates) will improve contrast. I have not used K-ferricyanide to improve osmium staining. Some labs use a little bit of picric acid as a protein coagulator I suppose. Plenty of people get good results without it.
Geoff
TindallR-at-missouri.edu wrote:
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-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 10, 35 -- From mcauliff-at-umdnj.edu Wed Jan 24 14:30:54 2007 10, 35 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 10, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0OKUrTU029852 10, 35 -- for {microscopy-at-msa.microscopy.com} ; Wed, 24 Jan 2007 14:30:54 -0600 10, 35 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 10, 35 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 6A61E1C3348 10, 35 -- for {microscopy-at-msa.microscopy.com} ; Wed, 24 Jan 2007 15:30:52 -0500 (EST) 10, 35 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) 10, 35 -- by zix01.umdnj.edu (Proprietary) with ESMTP id AD722A7B9B 10, 35 -- for {microscopy-at-msa.microscopy.com} ; Wed, 24 Jan 2007 15:30:50 -0500 (EST) 10, 35 -- Received: from ([130.219.34.131]) 10, 35 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.70631915; 10, 35 -- Wed, 24 Jan 2007 15:30:29 -0500 10, 35 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 10, 35 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 10, 35 -- id {0JCE00I013A2PW-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 10, 35 -- for microscopy-at-msa.microscopy.com; Wed, 24 Jan 2007 15:30:29 -0500 (EST) 10, 35 -- Received: from [127.0.0.1] ([10.138.2.123]) 10, 35 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 10, 35 -- 2004)) with ESMTP id {0JCE00AMD3ML7H-at-Polaris.umdnj.edu} ; Wed, 10, 35 -- 24 Jan 2007 15:30:21 -0500 (EST) 10, 35 -- Date: Wed, 24 Jan 2007 15:31:45 -0500 10, 35 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 10, 35 -- Subject: Re: [Microscopy] TEM: Fixation of mouse brain tissue 10, 35 -- In-reply-to: {200701241945.l0OJjGp8008888-at-ns.microscopy.com} 10, 35 -- To: TindallR-at-missouri.edu, 10, 35 -- MicroscopyListserver {microscopy-at-msa.microscopy.com} 10, 35 -- Message-id: {45B7C231.2020903-at-umdnj.edu} 10, 35 -- MIME-version: 1.0 10, 35 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 10, 35 -- Content-transfer-encoding: 7BIT 10, 35 -- X-Accept-Language: en-us, en 10, 35 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 10, 35 -- Gecko/20040804 Netscape/7.2 (ax) 10, 35 -- References: {200701241945.l0OJjGp8008888-at-ns.microscopy.com} ==============================End of - Headers==============================
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Email: smythen-at-musc.edu Name: Nancy Smythe
Organization: Medical University of South Carolina
Title-Subject: [Filtered] vigor tweezer sharpener
Question: Good luck. I bought one about 6 years ago without instructions. But I do get results, so if you don't get the instructions let me know and I will tell you my techniques and you can share what you know and between the two of us we can maybe get it straight!
On Jan 24, 2007, at 4:40 PM, smythen-at-musc.edu wrote:
} Good luck. I bought one about 6 years ago without instructions. But } I do get results, so if you don't get the instructions let me know and } I will tell you my techniques and you can share what you know and } between the two of us we can maybe get it straight! } Dear Jim & Nancy, Could you please post your techniques to the list? TIA. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 24 -- From tivol-at-caltech.edu Wed Jan 24 19:02:20 2007 4, 24 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0P12K85024375 4, 24 -- for {microscopy-at-msa.microscopy.com} ; Wed, 24 Jan 2007 19:02:20 -0600 4, 24 -- Received: from earth-dog.caltech.edu (earth-dog [192.168.1.3]) 4, 24 -- by earth-ox-postvirus (Postfix) with ESMTP 4, 24 -- id EEBAF10A099; Wed, 24 Jan 2007 17:02:19 -0800 (PST) 4, 24 -- Received: from [192.168.159.233] (jpix-01.caltech.edu [131.215.2.133]) 4, 24 -- (using TLSv1 with cipher RC4-SHA (128/128 bits)) 4, 24 -- (No client certificate requested) 4, 24 -- by water-ox.its.caltech.edu (Postfix) with ESMTP 4, 24 -- id 093E52EEEE; Wed, 24 Jan 2007 17:02:19 -0800 (PST) 4, 24 -- Mime-Version: 1.0 (Apple Message framework v624) 4, 24 -- In-Reply-To: {200701250040.l0P0et4L013176-at-ns.microscopy.com} 4, 24 -- References: {200701250040.l0P0et4L013176-at-ns.microscopy.com} 4, 24 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 24 -- Message-Id: {c986f3c20b4cc3e981ac867fdf0820cb-at-caltech.edu} 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 24 -- Subject: Re: [Microscopy] viaWWW: vigor tweezer sharpener 4, 24 -- Date: Wed, 24 Jan 2007 17:10:13 -0800 4, 24 -- To: microscopy-at-msa.microscopy.com, smythen-at-musc.edu 4, 24 -- X-Mailer: Apple Mail (2.624) 4, 24 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.3.3 ==============================End of - Headers==============================
In the 'ready' state all is well, with all the valve lights as they should be, and a pressure of around 10 - 5 in the chamber (there are 3 WDS spectros leaking away into the chamber) and around 5 x 10 - 6 in the gun.
When I push the GUN button to vent the gun, the lights do what they should, including the opening of LV1, but the gun pressure comes not up to atmospheric but to about 100 mbar, and the pressure in the vacuum ballast tank starts climbing, until the PiG3 light comes on and the system devotes itself, as it should, to re-evacuating the ballast tank.
Am I correct in guessing that V1B, the gun pumpdown valve, is not closing properly and is allowing the atmosphere to leak via LV1, the gun, and V1B, into the ballast tank?
I would welcome support (or, I suppose, otherwise) in this guess before I start ripping things apart.
There is a time constraint here, as we have the NZ EM conference in 10 days time and there's to be a teaching workshop involving the 840.
Help!
cheers
rtch
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 12, 27 -- From r.sims-at-auckland.ac.nz Wed Jan 24 19:30:06 2007 12, 27 -- Received: from harpo.itss.auckland.ac.nz (harpo.itss.auckland.ac.nz [130.216.190.13]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0P1U513003330 12, 27 -- for {microscopy-at-msa.microscopy.com} ; Wed, 24 Jan 2007 19:30:06 -0600 12, 27 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 12, 27 -- by harpo.itss.auckland.ac.nz (Postfix) with ESMTP id 5A6193485B 12, 27 -- for {microscopy-at-msa.microscopy.com} ; Thu, 25 Jan 2007 14:30:04 +1300 (NZDT) 12, 27 -- Received: from harpo.itss.auckland.ac.nz ([127.0.0.1]) 12, 27 -- by localhost (smtpc.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 12, 27 -- with ESMTP id 23181-05 for {microscopy-at-msa.microscopy.com} ; 12, 27 -- Thu, 25 Jan 2007 14:30:04 +1300 (NZDT) 12, 27 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) 12, 27 -- by harpo.itss.auckland.ac.nz (Postfix) with ESMTP id 317AE3485A 12, 27 -- for {microscopy-at-msa.microscopy.com} ; Thu, 25 Jan 2007 14:30:03 +1300 (NZDT) 12, 27 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 12, 27 -- To: microscopy-at-msa.microscopy.com 12, 27 -- Date: Thu, 25 Jan 2007 14:30:24 +1300 12, 27 -- MIME-Version: 1.0 12, 27 -- Subject: 840 vacuum problem 12, 27 -- Message-ID: {45B8BF00.12939.1379784-at-localhost} 12, 27 -- Priority: normal 12, 27 -- In-reply-to: {200701250103.l0P135c6026032-at-ns.microscopy.com} 12, 27 -- X-mailer: Pegasus Mail for Windows (4.21c) 12, 27 -- Content-type: text/plain; charset=US-ASCII 12, 27 -- Content-transfer-encoding: 7BIT 12, 27 -- Content-description: Mail message body 12, 27 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
Hi Randy, Geoff McCauliff's tips are my experiences too. a minute comment: use gravity perfusion; a wonderful & detailed instruction is given in:
Forssmann, W.G., Ito, S., Weihe, E., Aoki, A., Dym, M. and Fawcett, D.W. (1977) Anat Rec 188, 307-14.
a small but important tip: Filtrate ALL solutions used for perfusion through a 1 (or 2) mikrometer filter before use!
If you ever have seen a capillary in cross section and measured its diameter, you know why.
Use the two resp. tree-step technique described in the paper, in my experience perfusion with the "rinse" solution for 20-30 seconds followed by a single or two-step perfusion (3-5 minutes) with a mixture of freshly (!) prepared form- and glutaraldehyde (the latter prepared by the manufacturer according to the Anderson-technique) is sufficient for an adult wildtype mouse.
good luck, peter heimann
==============================Original Headers============================== 7, 31 -- From peter.heimann-at-uni-bielefeld.de Thu Jan 25 02:46:56 2007 7, 31 -- Received: from smtp-relay.uni-bielefeld.de (mux2-unibi-smtp.hrz.uni-bielefeld.de [129.70.204.73]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0P8ktmQ031325 7, 31 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jan 2007 02:46:56 -0600 7, 31 -- Received: from pmxchannel-daemon.mux2-unibi-smtp.hrz.uni-bielefeld.de by 7, 31 -- mux2-unibi-smtp.hrz.uni-bielefeld.de 7, 31 -- (Sun Java System Messaging Server 6.2-8.02 (built Dec 21 2006)) 7, 31 -- id {0JCF003011Q6FR00-at-mux2-unibi-smtp.hrz.uni-bielefeld.de} for 7, 31 -- microscopy-at-microscopy.com; Thu, 25 Jan 2007 09:46:54 +0100 (CET) 7, 31 -- Received: from [129.70.82.133] by mux2-unibi-smtp.hrz.uni-bielefeld.de 7, 31 -- (Sun Java System Messaging Server 6.2-8.02 (built Dec 21 2006)) 7, 31 -- with ESMTP id {0JCF000A41Q5BH80-at-mux2-unibi-smtp.hrz.uni-bielefeld.de} for 7, 31 -- microscopy-at-microscopy.com; Thu, 25 Jan 2007 09:46:54 +0100 (CET) 7, 31 -- Date: Thu, 25 Jan 2007 09:46:52 +0100 7, 31 -- From: PETER HEIMANN {peter.heimann-at-uni-bielefeld.de} 7, 31 -- Subject: Re: TEM: Fixation of mouse brain tissue 7, 31 -- In-reply-to: {200701241944.l0OJiRh8007561-at-ns.microscopy.com} 7, 31 -- To: microscopy-at-microscopy.com 7, 31 -- Reply-to: peter.heimann-at-uni-bielefeld.de 7, 31 -- Message-id: {45B86E7C.20404-at-UNI-BIELEFELD.DE} 7, 31 -- Organization: Uni Bielefeld 7, 31 -- MIME-version: 1.0 7, 31 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 7, 31 -- Content-transfer-encoding: 7BIT 7, 31 -- X-Accept-Language: en-us, en 7, 31 -- X-EnvFrom: peter.heimann-at-uni-bielefeld.de 7, 31 -- X-PMX-Version: 5.2.1.279297, Antispam-Engine: 2.5.0.283055, 7, 31 -- Antispam-Data: 2007.1.25.3433, pmx1.hrz.uni-bielefeld.de 7, 31 -- X-Connecting-IP: 127.0.0.1 7, 31 -- References: {200701241944.l0OJiRh8007561-at-ns.microscopy.com} 7, 31 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) ==============================End of - Headers==============================
Vacuum systems have high and low speed pumping areas their problems show up as set out below, high (1) and low (2).
1. If a reasonable vacuum is held in the high vacuum area but the rotary pump or the backing tank show a considerable decrease in their vacuum level, the problem is in an area with close proximity to a main pumping line
2. If a poor vacuum is held in the high vacuum area and the rotary pump or the backing tank also show a considerable decrease in its vacuum level, the problem is in an area with some distance (vacuum wise) to a main pumping line.
Always ask what did I do last and it seems that here lies the problem, first reverse the action does the system recover? It would suggest a leak between the gun, trying to reach atmospheric, and the column trying to retain its high vacuum.
Good luck
Steve Chapman Senior Consultant Protrain For electron microscopy consultancy and training world wide Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967 Web www.emcourses.com
----- Original Message ----- X-from: {r.sims-at-auckland.ac.nz} To: {protrain-at-emcourses.com} Sent: Thursday, January 25, 2007 1:30 AM
We found that the red (typically rhodamine) images taken with our Zeiss Axiocam MRc are fuzzy. We looked at the blue and found this a problem too, but since in fluorescence people typically use the blue just as a marker for nucleii rather than as stain for fine structure, they didn't notice this problem. (Illustrations at http://www.aecom.yu.edu/aif/temp/axiocamproblem/ ).
This made us worry about the sharpness of images of histological stained samples, such as H&E.
First we checked that this isn't a problem due to the fluorescent filter sets. To do this, we filtered the light to be red, green or blue at the halogen lamp using RGB filters removed from an old 35mm film recorder and imaged the sample with no filters in the light path between the sample and the camera. The result was that the red and blue images were very fuzzy.
Shooting in the b/w mode solves this problem, but then the images are not color. Also, this may solve the problem for fluorescence, but what about brightfield or other techniques that involve colors?
Can anyone explain why this happens and how to fix it? Is this a problem with our sytem uniquely or is this a problem with the Axiocam in general?
Thanks!
-Michael ____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. URL: http://www.aecom.yu.edu/aif/
==============================Original Headers============================== 8, 29 -- From cammer-at-aecom.yu.edu Thu Jan 25 10:17:49 2007 8, 29 -- Received: from mx1.aecom.yu.edu (mx1.aecom.yu.edu [129.98.1.51]) 8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0PGHnp3030006 8, 29 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jan 2007 10:17:49 -0600 8, 29 -- Received: from draco.aecom.yu.edu (draco.aecom.yu.edu [129.98.1.160]) 8, 29 -- by mx1.aecom.yu.edu (Postfix) with ESMTP id 5A9FA9F00EF; 8, 29 -- Thu, 25 Jan 2007 11:17:49 -0500 (EST) 8, 29 -- Received: from draco.aecom.yu.edu (unknown [127.0.0.1]) 8, 29 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 8D5C38B4006; 8, 29 -- Thu, 25 Jan 2007 11:06:04 -0500 (EST) 8, 29 -- X-AuditID: 816201a0-a13c8bb000007022-82-45b8d56c712a 8, 29 -- Received: from post.aecom.yu.edu (post.aecom.yu.edu [129.98.1.100]) 8, 29 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 4E7FE718002; 8, 29 -- Thu, 25 Jan 2007 11:06:04 -0500 (EST) 8, 29 -- Received: from AIF3.aecom.yu.edu (aif3.aif.aecom.yu.edu [129.98.80.70]) 8, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 8, 29 -- (No client certificate requested) 8, 29 -- by post.aecom.yu.edu (Postfix) with ESMTP id C3E7E26; 8, 29 -- Thu, 25 Jan 2007 11:17:43 -0500 (EST) 8, 29 -- Message-Id: {7.0.1.0.2.20070125111540.04f87920-at-aecom.yu.edu} 8, 29 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 8, 29 -- Date: Thu, 25 Jan 2007 11:17:43 -0500 8, 29 -- To: microscopy-at-microscopy.com 8, 29 -- From: Michael Cammer {cammer-at-aecom.yu.edu} 8, 29 -- Subject: color CCD Zeiss AxioCam MRc problem 8, 29 -- Cc: yadeng-at-aecom.yu.edu, rleonard-at-aecom.yu.edu, jmorreale-at-zeiss.com 8, 29 -- Mime-Version: 1.0 8, 29 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 29 -- X-Brightmail-Tracker: AAAAAA== ==============================End of - Headers==============================
If you use the "VENT" button rather than the "GUN VENT" to vent the whole column and the ballast pressure holds up, your problem is most likely that the V7 valve O-ring is leaking between the gun and specimen chambers. This would also eliminate V1B as a possibility.
Unfortunately, you will have to tear down the column to get to the V7 O-ring. The good news is, this valve should only present a problem when doing a gun vent. To avoid introducing more serious problems I would just use the VENT button temporarily when needed and postpone the repair until after your conference.
Regards, Bill
On 1/24/07, r.sims-at-auckland.ac.nz {r.sims-at-auckland.ac.nz} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Here's one for all the 840 afficianados: } } In the 'ready' state all is well, with all the valve lights as they should be, and a } pressure of around 10 - 5 in the chamber (there are 3 WDS spectros leaking away } into the chamber) and around 5 x 10 - 6 in the gun. } } When I push the GUN button to vent the gun, the lights do what they should, including } the opening of LV1, but the gun pressure comes not up to atmospheric but to about } 100 mbar, and the pressure in the vacuum ballast tank starts climbing, until the PiG3 } light comes on and the system devotes itself, as it should, to re-evacuating the ballast } tank. } } Am I correct in guessing that V1B, the gun pumpdown valve, is not closing properly } and is allowing the atmosphere to leak via LV1, the gun, and V1B, into the ballast } tank? } } I would welcome support (or, I suppose, otherwise) in this guess before I start ripping } things apart. } } There is a time constraint here, as we have the NZ EM conference in 10 days time } and there's to be a teaching workshop involving the 840. } } Help! } } cheers } } rtch } } -- } Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 } Microanalyst Fax : 64 9 3737435 } Department of Geology email : r.sims-at-auckland.ac.nz } The University of Auckland } Private Bag 92019 } Auckland } New Zealand } } } ==============================Original Headers============================== } 12, 27 -- From r.sims-at-auckland.ac.nz Wed Jan 24 19:30:06 2007 } 12, 27 -- Received: from harpo.itss.auckland.ac.nz (harpo.itss.auckland.ac.nz [130.216.190.13]) } 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0P1U513003330 } 12, 27 -- for {microscopy-at-msa.microscopy.com} ; Wed, 24 Jan 2007 19:30:06 -0600 } 12, 27 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 12, 27 -- by harpo.itss.auckland.ac.nz (Postfix) with ESMTP id 5A6193485B } 12, 27 -- for {microscopy-at-msa.microscopy.com} ; Thu, 25 Jan 2007 14:30:04 +1300 (NZDT) } 12, 27 -- Received: from harpo.itss.auckland.ac.nz ([127.0.0.1]) } 12, 27 -- by localhost (smtpc.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) } 12, 27 -- with ESMTP id 23181-05 for {microscopy-at-msa.microscopy.com} ; } 12, 27 -- Thu, 25 Jan 2007 14:30:04 +1300 (NZDT) } 12, 27 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) } 12, 27 -- by harpo.itss.auckland.ac.nz (Postfix) with ESMTP id 317AE3485A } 12, 27 -- for {microscopy-at-msa.microscopy.com} ; Thu, 25 Jan 2007 14:30:03 +1300 (NZDT) } 12, 27 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} } 12, 27 -- To: microscopy-at-msa.microscopy.com } 12, 27 -- Date: Thu, 25 Jan 2007 14:30:24 +1300 } 12, 27 -- MIME-Version: 1.0 } 12, 27 -- Subject: 840 vacuum problem } 12, 27 -- Message-ID: {45B8BF00.12939.1379784-at-localhost} } 12, 27 -- Priority: normal } 12, 27 -- In-reply-to: {200701250103.l0P135c6026032-at-ns.microscopy.com} } 12, 27 -- X-mailer: Pegasus Mail for Windows (4.21c) } 12, 27 -- Content-type: text/plain; charset=US-ASCII } 12, 27 -- Content-transfer-encoding: 7BIT } 12, 27 -- Content-description: Mail message body } 12, 27 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz } ==============================End of - Headers============================== }
-- William J Mushock Lehigh University Whitaker Laboratory 5 East Packer Ave. Bethlehem, PA 18015 (610)-758-4283
==============================Original Headers============================== 6, 26 -- From bill.mushock-at-gmail.com Thu Jan 25 11:05:58 2007 6, 26 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.238]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0PH5vu1009442 6, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Jan 2007 11:05:58 -0600 6, 26 -- Received: by wx-out-0506.google.com with SMTP id s16so560115wxc 6, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Jan 2007 09:05:57 -0800 (PST) 6, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 26 -- d=gmail.com; s=beta; 6, 26 -- h=received:message-id:date:from:sender:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references:x-google-sender-auth; 6, 26 -- b=h0j2UNl6VAYGJsJMfcp6v4S6qjd3GX2nVyxcRD1aMv1iWfUnmyVZqRueusr+RCakUcYzcwEYfi47DV+xGNb0YL9S6D4qj9Few4Tc2PwMrJ9s2u727BsUP1Ii9OjoJiXwOW3INQKMRfxu+cPJrH6LXK2Vc1mvqQLQbs4W96Sxerc= 6, 26 -- Received: by 10.70.52.5 with SMTP id z5mr4060864wxz.1169744757551; 6, 26 -- Thu, 25 Jan 2007 09:05:57 -0800 (PST) 6, 26 -- Received: by 10.70.36.7 with HTTP; Thu, 25 Jan 2007 09:05:57 -0800 (PST) 6, 26 -- Message-ID: {61452d440701250905w34ff2e77j4a56c18c6f15e33c-at-mail.gmail.com} 6, 26 -- Date: Thu, 25 Jan 2007 12:05:57 -0500 6, 26 -- From: "William J Mushock" {wim5-at-lehigh.edu} 6, 26 -- Sender: bill.mushock-at-gmail.com 6, 26 -- To: Microscopy-at-microscopy.com 6, 26 -- Subject: Re: [Microscopy] 840 vacuum problem 6, 26 -- In-Reply-To: {200701250132.l0P1Whdp007019-at-ns.microscopy.com} 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 26 -- Content-Transfer-Encoding: 7bit 6, 26 -- Content-Disposition: inline 6, 26 -- References: {200701250132.l0P1Whdp007019-at-ns.microscopy.com} 6, 26 -- X-Google-Sender-Auth: 7b3ead8b693005a4 ==============================End of - Headers==============================
I am trying to order photographic paper racks which are used inside a dryer box after printing. If anyone knows were I can buy them, please let me know. Thanks,
ANI M ISSAIAN Electron Microscopy Facility Manager California State University, Northridge 18111 Nordhoff street, Northridge, CA 91330-8303 Biology dept., MC 8303, Room CS2205 Phone: (818) 677-3383 Fax (818) 677-2034
==============================Original Headers============================== 4, 28 -- From ani.issaian-at-csun.edu Thu Jan 25 11:14:15 2007 4, 28 -- Received: from plover.csun.edu (plover.csun.edu [130.166.1.24]) 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0PHEDfV020498 4, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Jan 2007 11:14:14 -0600 4, 28 -- Received: from puffin.csun.edu (puffin.csun.edu [130.166.1.21]) 4, 28 -- by plover.csun.edu (MOS 3.8.2-GA) 4, 28 -- with ESMTP id DMG61952 4, 28 -- for {Microscopy-at-microscopy.com} ; 4, 28 -- Thu, 25 Jan 2007 09:14:11 -0800 (PST) 4, 28 -- Received: from cuckoo.csun.edu (cuckoo.csun.edu [130.166.1.230]) 4, 28 -- by puffin.csun.edu (MOS 3.7.5a-GA) 4, 28 -- with ESMTP id FJW26576 4, 28 -- for {Microscopy-at-microscopy.com} ; 4, 28 -- Thu, 25 Jan 2007 09:14:10 -0800 (PST) 4, 28 -- Received: (from cuckoo.csun.edu [130.166.114.202]) 4, 28 -- by cuckoo.csun.edu (MOS 3.8.2-GA) 4, 28 -- with HTTPS/1.1 id ARZ65214 (AUTH ami24015); 4, 28 -- Thu, 25 Jan 2007 09:14:10 -0800 (PST) 4, 28 -- From: Ani M Issaian {ani.issaian-at-csun.edu} 4, 28 -- To: Microscopy-at-microscopy.com 4, 28 -- Reply-To: ani.issaian-at-csun.edu 4, 28 -- X-Mailer: Mirapoint Webmail Direct 3.8.2-GA 4, 28 -- MIME-Version: 1.0 4, 28 -- Content-Type: text/plain; charset=us-ascii 4, 28 -- Content-Transfer-Encoding: 7bit 4, 28 -- Message-Id: {20070125091410.ARZ65214-at-cuckoo.csun.edu} 4, 28 -- Date: Thu, 25 Jan 2007 09:14:10 -0800 (PST) 4, 28 -- X-Junkmail-Whitelist: YES (by domain whitelist at plover.csun.edu) ==============================End of - Headers==============================
All, Can anyone tell me of a source for a nitrogen free epoxy? I realize that may be a contradiction in terms, but I need to find a mounting media that can hold a water soluble polymer coated wire for cross sectioning for subsequent nitrogen analysis by EPMA.
Thanks! john
John J. Donovan donovan-at-uoregon.edu University of Oregon (541) 346-4632 (office) 1260 Franklin Blvd (541) 346-4655 (probe) Eugene, OR (541) 346-4778 (SEM) 97403-1272 (541) 346-4692 (FAX)
"Aristotle presented with a moon rock would have no difficulty in discerning it as an object not fundamentally different from terrestrial materials. So much for Thomas Kuhn."
"I'd rather be uncertain and wrong, than be certain and wrong."
==============================Original Headers============================== 9, 20 -- From donovan-at-uoregon.edu Thu Jan 25 11:24:37 2007 9, 20 -- Received: from smtp.uoregon.edu (mserv2.uoregon.edu [128.223.142.41]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0PHOadg031693 9, 20 -- for {Microscopy-at-Microscopy.Com} ; Thu, 25 Jan 2007 11:24:36 -0600 9, 20 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.92.75]) 9, 20 -- (authenticated bits=0) 9, 20 -- by smtp.uoregon.edu (8.13.8/8.13.8) with ESMTP id l0PHOahW007001 9, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 9, 20 -- for {Microscopy-at-Microscopy.Com} ; Thu, 25 Jan 2007 09:24:36 -0800 9, 20 -- Message-Id: {7.0.1.0.0.20070125092420.039399b8-at-uoregon.edu} 9, 20 -- Message-Id: {7.0.1.0.0.20070125091904.038e7cd0-at-uoregon.edu} 9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 9, 20 -- Date: Thu, 25 Jan 2007 09:24:32 -0800 9, 20 -- To: Microscopy-at-Microscopy.Com 9, 20 -- From: John Donovan {donovan-at-uoregon.edu} 9, 20 -- Subject: Nitrogen free epoxy? 9, 20 -- Mime-Version: 1.0 9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 9, 20 -- X-Virus-Scanned: ClamAV 0.88.7/2489/Thu Jan 25 04:35:24 2007 on mserv2 9, 20 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Our current computer (Acer Altos 11000) that controls FEI's Tecnai 12 TEM is having serious problems. I am looking for an used Acer Altos 11000, or 12000, or 21000 to replace our current one. If you have one for sale or if you would like to upgrade yours, could you please let me know? I will pay for the computer, handling and shipping cost.
Thank you, Ping
-- Ping Li, Ph.D. Director, Scientific Imaging Suite Department of Biology Dalhousie University Halifax, NS B3H 4J1 Canada
With somewhat mixed emotions, we finally decommissioned our darkroom and transferred the (nearly new) 4x5 setup to the Fine Arts Department. This move was prompted by the upgrade of our JEOL 5600 computer system, which essentially disabled the photo system (not that we ever used it). On a recent service visit I had the engineer remove the system entirely. JEOL Canada doesn't have any interest in retrieving it, so on the off chance that somebody desperately needs a replacement, I'm offering it to anyone who will pay shipping for the thing. Let me know relatively quickly - it's probably destined for the dumpster if I can't find someone to give it to. There's a new Polaroid back on it, if that makes it any more attractive.
Cheers,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University Sackville, NB E4L 1G7 CANADA
The College of Staten Island of The City University of New York has an opening for a Research Associate to serve as a Facilities Manager for the Program in Neuroscience and Advanced Imaging Facility. A description of the position can be found at:
William L'Amoreaux, Ph.D. Associate Professor and Deputy Chair Director, Advanced Imaging Facility CUNY College of Staten Island 2800 Victory Blvd. Staten Island, NY 10314 (718) 982-3864 - office (718) 982-3852
==============================Original Headers============================== 5, 20 -- From lamoreaux-at-mail.csi.cuny.edu Thu Jan 25 14:40:10 2007 5, 20 -- Received: from mail.csi.cuny.edu (mail.csi.cuny.edu [163.238.8.100]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0PKe9JI003792 5, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Jan 2007 14:40:09 -0600 5, 20 -- Received: from D7K4H8C1 [163.238.22.9] by mail.csi.cuny.edu with ESMTP 5, 20 -- (SMTPD-8.22) id A5A90440; Thu, 25 Jan 2007 15:40:09 -0500 5, 20 -- Reply-To: {lamoreaux-at-mail.csi.cuny.edu} 5, 20 -- From: "William L'Amoreaux" {lamoreaux-at-mail.csi.cuny.edu} 5, 20 -- To: {Microscopy-at-microscopy.com} 5, 20 -- Subject: research associate postion 5, 20 -- Date: Thu, 25 Jan 2007 15:40:05 -0500 5, 20 -- Organization: College of Staten Island 5, 20 -- Message-ID: {001c01c740c0$febe95b0$0916eea3-at-D7K4H8C1} 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; 5, 20 -- charset="us-ascii" 5, 20 -- Content-Transfer-Encoding: 7bit 5, 20 -- X-Mailer: Microsoft Office Outlook 11 5, 20 -- thread-index: AcdAwP6ki/NffyhDTIecNLGTCYQiEg== 5, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 ==============================End of - Headers==============================
Twelfth Annual INTERNATIONAL 12-Day Short Course on 3D Microscopy of Living Cells, June 17 - 28, 2007 (Pre-course: June 16)
Eleventh, Post-course Workshop on 3D Image Processing, June 30 -July 2
Organized by Prof. James Pawley, (University of Wisconsin-Madison)
in association with the Departments of Pharmacology and Physiology and the Brain Research Centre, University of British Columbia, Vancouver, BC, Canada
DATES
Applications must be received by Tuesday, March 15, 2007 Deposit due Friday, April 15, 2007 Registration 5:00 - 7:00 PM Saturday, June 16, 2007 First Lecture 7:30 PM Saturday, June 16, 2007 Live-cell Course ends, noon Thursday, June 28, 2007 3D Image Processing Course, Saturday, June 30 - Monday, July 2, 2007
APPLICATIONS DUE BY MARCH 15, 2007
APPLICATIONS Applicants must complete a questionnaire on the web to assess knowledge level, field of interest and proposed personal, live-cell, project. But don't let this put you off: if you plan to use 3D microscopy on living cells, we can usually find a way to make it work.
Enrollment will be limited to about 32 participants (exact number depends on number of 3D Systems available). Selection will be made on the basis of background and perceived need. Those without previous LM experience will be provided with access to basic texts to read before the course begins. Application forms may be down-loaded from the WWW site at
http://www.3dcourse.ubc.ca/application.htm , or obtained from:
Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 250 N. Mills Street, Madison, WI, 53706 JBPAWLEY-at-WISC.EDU
Additional information is available from: http://www.3dcourse.ubc.ca/brochure.htm and links.
We expect to have at least 13, 3D microscope workstations for student use and there will be an international faculty of 22.
Application deadlines:
Application forms should be received for screening by March 15, 2007. Successful applicants will be notified by April 1, and a deposit of 50% must be received by April 15, 2007. In general, refunds of the deposit will only be possible if someone on the waiting list can take your place but this has not been a problem in previous years. The remaining balance is due before Registration.
Pre-Course Tuition (1/2 day Basic Optical principles) $150 (US) 3D Live-cell Course Tuition (includes lunches, 2 big snacks, 3 dinners, incl. the NEW Third Edition of the Handbook of Biological Confocal Microscopy): $2,850 (US) Workshop Tuition (includes lunches, snacks, final dinner): $1,200 (US)
Room/board about $40/day (US) depending on room type.
I hope that this includes all of the information that you need, but if not, please get back to me.
Jim Pawley
-- **************************************** Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU "A scientist is not one who can answer questions but one who can question answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39
==============================Original Headers============================== 23, 12 -- From zaluzec-at-microscopy.com Thu Jan 25 19:18:49 2007 23, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 23, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0Q1ImSV019471 23, 12 -- for {microscopy-at-microscopy.com} ; Thu, 25 Jan 2007 19:18:49 -0600 23, 12 -- Mime-Version: 1.0 23, 12 -- X-Sender: (Unverified) 23, 12 -- Message-Id: {p06110400c1df0711bdc4-at-[206.69.208.22]} 23, 12 -- Date: Thu, 25 Jan 2007 19:18:48 -0600 23, 12 -- To: microscopy-at-microscopy.com 23, 12 -- From: James Pawley {jbpawley-at-wisc.edu} (by way of MicroscopyListserver) 23, 12 -- Subject: Twelfth International UBC 3D LiveCell Course 23, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Hi rtch, The problem could well be V1B or V7 (column isolation). I haven't had much opportunity to work on JEOL spectrometers, but the question arises: is there a thin window detector and could its vacuum manifold be part of the problem?
The part that bothers me the most is that the gun is being held to about 100mB (75 Torr) while being vented (this is a huge gas load to handle and maintain that kind of pressure) and PiG 3 is ***not*** being dumped instantaneously along with a big blast of DP oil being sent into the specimen chamber.
I would be looking for a combination of a leak in V1B or V7 and either a restriction in the nitrogen line to LV1 or perhaps its timer is shutting it very quickly.
Btw what additional gauging have you got that would let you know that the gun is at about 100mB? Do you have a convection gauge or Bourdon Tube gauge attached to the gun?
I also strongly second Jim's comment about "what was the last thing you did before you had the problem?", but I bet you already know that.
A very interesting problem. Keep us posted on what you find.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: r.sims-at-auckland.ac.nz [mailto:r.sims-at-auckland.ac.nz] Sent: Wednesday, January 24, 2007 8:33 PM To: kenconverse-at-qualityimages.biz
Here's one for all the 840 afficianados:
In the 'ready' state all is well, with all the valve lights as they should be, and a pressure of around 10 - 5 in the chamber (there are 3 WDS spectros leaking away into the chamber) and around 5 x 10 - 6 in the gun.
When I push the GUN button to vent the gun, the lights do what they should, including the opening of LV1, but the gun pressure comes not up to atmospheric but to about 100 mbar, and the pressure in the vacuum ballast tank starts climbing, until the PiG3 light comes on and the system devotes itself, as it should, to re-evacuating the ballast tank.
Am I correct in guessing that V1B, the gun pumpdown valve, is not closing properly and is allowing the atmosphere to leak via LV1, the gun, and V1B, into the ballast tank?
I would welcome support (or, I suppose, otherwise) in this guess before I start ripping things apart.
There is a time constraint here, as we have the NZ EM conference in 10 days time and there's to be a teaching workshop involving the 840.
Help!
cheers
rtch
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 12, 27 -- From r.sims-at-auckland.ac.nz Wed Jan 24 19:30:06 2007 12, 27 -- Received: from harpo.itss.auckland.ac.nz (harpo.itss.auckland.ac.nz [130.216.190.13]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0P1U513003330 12, 27 -- for {microscopy-at-msa.microscopy.com} ; Wed, 24 Jan 2007 19:30:06 -0600 12, 27 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 12, 27 -- by harpo.itss.auckland.ac.nz (Postfix) with ESMTP id 5A6193485B 12, 27 -- for {microscopy-at-msa.microscopy.com} ; Thu, 25 Jan 2007 14:30:04 +1300 (NZDT) 12, 27 -- Received: from harpo.itss.auckland.ac.nz ([127.0.0.1]) 12, 27 -- by localhost (smtpc.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 12, 27 -- with ESMTP id 23181-05 for {microscopy-at-msa.microscopy.com} ; 12, 27 -- Thu, 25 Jan 2007 14:30:04 +1300 (NZDT) 12, 27 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) 12, 27 -- by harpo.itss.auckland.ac.nz (Postfix) with ESMTP id 317AE3485A 12, 27 -- for {microscopy-at-msa.microscopy.com} ; Thu, 25 Jan 2007 14:30:03 +1300 (NZDT) 12, 27 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 12, 27 -- To: microscopy-at-msa.microscopy.com 12, 27 -- Date: Thu, 25 Jan 2007 14:30:24 +1300 12, 27 -- MIME-Version: 1.0 12, 27 -- Subject: 840 vacuum problem 12, 27 -- Message-ID: {45B8BF00.12939.1379784-at-localhost} 12, 27 -- Priority: normal 12, 27 -- In-reply-to: {200701250103.l0P135c6026032-at-ns.microscopy.com} 12, 27 -- X-mailer: Pegasus Mail for Windows (4.21c) 12, 27 -- Content-type: text/plain; charset=US-ASCII 12, 27 -- Content-transfer-encoding: 7BIT 12, 27 -- Content-description: Mail message body 12, 27 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
_________________________________________________________________ Need personalized email and website? Look no further. It's easy with Doteasy $0 Web Hosting! Learn more at www.doteasy.com
==============================Original Headers============================== 34, 28 -- From kenconverse-at-qualityimages.biz Thu Jan 25 19:43:35 2007 34, 28 -- Received: from dpmailmta02.doteasy.com (dpmailmta02.doteasy.com [65.61.209.88]) 34, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0Q1hZAY030854 34, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 25 Jan 2007 19:43:35 -0600 34, 28 -- Received: from qualityimages.biz (unverified [192.168.101.16]) 34, 28 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id 52497752-1814644 34, 28 -- for multiple; Thu, 25 Jan 2007 18:16:15 -0800 34, 28 -- Received: from Ken [76.179.237.214] by qualityimages.biz with ESMTP 34, 28 -- (SMTPD32-8.05) id AD2972C0138; Thu, 25 Jan 2007 17:45:13 -0800 34, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 34, 28 -- To: {r.sims-at-auckland.ac.nz} , "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 34, 28 -- Subject: RE: [Microscopy] 840 vacuum problem 34, 28 -- Date: Thu, 25 Jan 2007 20:43:06 -0500 34, 28 -- Message-ID: {002201c740eb$55a753b0$6401a8c0-at-Ken} 34, 28 -- MIME-Version: 1.0 34, 28 -- Content-Type: text/plain; 34, 28 -- charset="us-ascii" 34, 28 -- X-Priority: 3 (Normal) 34, 28 -- X-MSMail-Priority: Normal 34, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 34, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 34, 28 -- Importance: Normal 34, 28 -- In-Reply-To: {200701250133.l0P1XAjA008071-at-ns.microscopy.com} 34, 28 -- X-IMSTrailer: __IMail_7__ 34, 28 -- X-IP-stats: Incoming Last 0, First 120, in=2905538, out=0, spam=0 ip=192.168.101.16 34, 28 -- X-External-IP: 192.168.101.16 34, 28 -- Content-Transfer-Encoding: 8bit 34, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0Q1hZAY030854 ==============================End of - Headers==============================
We have the same camera and I must say that I never noticed that. We have the B/W axiocam too and since this one is more sensitive than the color one, I usually use it for fluorescence work. Of course each wavelength having a specific convergence angle it is necessary to refocus each time you change the filter, however this does not explain why you get a sharp image in BW mode (except if you refocused in this mode). The color camera is used for more "classical" stainings like HE. In general, I find the image pretty grainy. For example the digital zoom is absolutely useless because the image becomes too "pixely". Perhaps it is becaused I am used to the high definition of TEM images, perhaps it is because I do most of the LM work on single cell imaging (reaching the physical limits of LM). To answer this question rationally I should try a higher resolution camera, however I am pretty sure I won't have the credits just to answer this question ;-) I questioned the Zeiss team about the quality of my images and they say it is normal, so I have to accept it somehow.
Regards,
Stephane
--- cammer-at-aecom.yu.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We found that the red (typically rhodamine) images } taken with our } Zeiss Axiocam MRc are fuzzy. We looked at the blue } and found this a } problem too, but since in fluorescence people } typically use the blue } just as a marker for nucleii rather than as stain } for fine structure, } they didn't notice this problem. (Illustrations at } http://www.aecom.yu.edu/aif/temp/axiocamproblem/ ). } } This made us worry about the sharpness of images of } histological } stained samples, such as H&E. } } First we checked that this isn't a problem due to } the fluorescent } filter sets. To do this, we filtered the light to } be red, green or } blue at the halogen lamp using RGB filters removed } from an old 35mm } film recorder and imaged the sample with no filters } in the light path } between the sample and the camera. The result was } that the red and } blue images were very fuzzy. } } Shooting in the b/w mode solves this problem, but } then the images are } not color. Also, this may solve the problem for } fluorescence, but } what about brightfield or other techniques that } involve colors? } } Can anyone explain why this happens and how to fix } it? Is this a } problem with our sytem uniquely or is this a problem } with the Axiocam } in general? } } Thanks! } } -Michael } ____________________________________________________________________________ } Michael Cammer Analytical Imaging Facility } Albert Einstein Coll. of Med. } URL: http://www.aecom.yu.edu/aif/ } } } ==============================Original } Headers============================== } 8, 29 -- From cammer-at-aecom.yu.edu Thu Jan 25 } 10:17:49 2007 } 8, 29 -- Received: from mx1.aecom.yu.edu } (mx1.aecom.yu.edu [129.98.1.51]) } 8, 29 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l0PGHnp3030006 } 8, 29 -- for {microscopy-at-microscopy.com} ; Thu, 25 } Jan 2007 10:17:49 -0600 } 8, 29 -- Received: from draco.aecom.yu.edu } (draco.aecom.yu.edu [129.98.1.160]) } 8, 29 -- by mx1.aecom.yu.edu (Postfix) with ESMTP } id 5A9FA9F00EF; } 8, 29 -- Thu, 25 Jan 2007 11:17:49 -0500 (EST) } 8, 29 -- Received: from draco.aecom.yu.edu (unknown } [127.0.0.1]) } 8, 29 -- by draco.aecom.yu.edu (Symantec Mail } Security) with ESMTP id 8D5C38B4006; } 8, 29 -- Thu, 25 Jan 2007 11:06:04 -0500 (EST) } 8, 29 -- X-AuditID: } 816201a0-a13c8bb000007022-82-45b8d56c712a } 8, 29 -- Received: from post.aecom.yu.edu } (post.aecom.yu.edu [129.98.1.100]) } 8, 29 -- by draco.aecom.yu.edu (Symantec Mail } Security) with ESMTP id 4E7FE718002; } 8, 29 -- Thu, 25 Jan 2007 11:06:04 -0500 (EST) } 8, 29 -- Received: from AIF3.aecom.yu.edu } (aif3.aif.aecom.yu.edu [129.98.80.70]) } 8, 29 -- (using TLSv1 with cipher } DHE-RSA-AES256-SHA (256/256 bits)) } 8, 29 -- (No client certificate requested) } 8, 29 -- by post.aecom.yu.edu (Postfix) with ESMTP } id C3E7E26; } 8, 29 -- Thu, 25 Jan 2007 11:17:43 -0500 (EST) } 8, 29 -- Message-Id: } {7.0.1.0.2.20070125111540.04f87920-at-aecom.yu.edu} } 8, 29 -- X-Mailer: QUALCOMM Windows Eudora Version } 7.0.1.0 } 8, 29 -- Date: Thu, 25 Jan 2007 11:17:43 -0500 } 8, 29 -- To: microscopy-at-microscopy.com } 8, 29 -- From: Michael Cammer {cammer-at-aecom.yu.edu} } 8, 29 -- Subject: color CCD Zeiss AxioCam MRc } problem } 8, 29 -- Cc: yadeng-at-aecom.yu.edu, } rleonard-at-aecom.yu.edu, jmorreale-at-zeiss.com } 8, 29 -- Mime-Version: 1.0 } 8, 29 -- Content-Type: text/plain; } charset="us-ascii"; format=flowed } 8, 29 -- X-Brightmail-Tracker: AAAAAA== } ==============================End of - } Headers============================== }
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==============================Original Headers============================== 9, 21 -- From nizets2-at-yahoo.com Fri Jan 26 05:18:28 2007 9, 21 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0QBIS9A018787 9, 21 -- for {microscopy-at-microscopy.com} ; Fri, 26 Jan 2007 05:18:28 -0600 9, 21 -- Received: (qmail 59506 invoked by uid 60001); 26 Jan 2007 11:18:27 -0000 9, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 21 -- s=s1024; d=yahoo.com; 9, 21 -- h=X-YMail-OSG:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 9, 21 -- b=5xtcf3ab0P0lvV3K+ARcllmK8q6VYiM3tFHdaaU9YMHNcynCgtt8AtrwkadChoB48V9fHRqgnjT2hFc80sg7QCTCUxdGGuVZ2dieoeTuh512WNx6H6DYINpd2aLwLEXgohXdfvfVvdpop6u5zg9cE2Wwyos45HA5OIej83X74Cw=; 9, 21 -- X-YMail-OSG: SZCGGSkVM1noy50s_7cZ2wnm8LdubtYip1L_5vpDShbX6U7dBg4FRh6O6uKFabbS1HS9sigpJZ_ZeFDNi4ox5DeVVRbvsBNAEV5GY7ehgdUTd2605rU1mIulPbxrFnVU09VtKetok5m6wGA13kX.WhyKaaX3wjd5l1fmckaPYiKo 9, 21 -- Received: from [80.122.101.102] by web37405.mail.mud.yahoo.com via HTTP; Fri, 26 Jan 2007 03:18:27 PST 9, 21 -- Date: Fri, 26 Jan 2007 03:18:27 -0800 (PST) 9, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 9, 21 -- Subject: Re: [Microscopy] color CCD Zeiss AxioCam MRc problem 9, 21 -- To: cammer-at-aecom.yu.edu 9, 21 -- Cc: microscopy-at-microscopy.com 9, 21 -- In-Reply-To: {200701251623.l0PGNVuH005535-at-ns.microscopy.com} 9, 21 -- MIME-Version: 1.0 9, 21 -- Content-Type: text/plain; charset=iso-8859-1 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- Message-ID: {648803.48936.qm-at-web37405.mail.mud.yahoo.com} ==============================End of - Headers==============================
I really know little of what you are doing, but I haven't seen any answers to your question so I thought I'd throw something back at you.
Can you use something other than epoxy? For example a polyester mounting material?
The polyester poly(ethyene terephthalate) doesn't have any nitrogen in it, for example. I think several other forms of polyesters also do not contain nitrogen, but I am no expert in that field.
There is a commercial product from Extec that is a polyester mount material. I don't know if it's nitrogen free, but you could ask them for the MSDS of the product and see. A link to their web page where they have their cold mounting supplies is:
http://www.extec.com/coldmounting.htm
Disclaimer: I have no commercial interest in this company or it's products.
I do use their polyester mounting material when I have samples I need to look at close to the edge with the mount and I don't want all the added elements usually found in epoxies. But I've not yet been concerned with nitrogen as one of those elements...
Good luck, and I would like to know what you do end up finding if you wouldn't mind posting your results...
dj
On Thu, 25 Jan 2007, donovan-at-uoregon.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } All, } Can anyone tell me of a source for a nitrogen free epoxy? I realize } that may be a contradiction in terms, but I need to find a mounting } media that can hold a water soluble polymer coated wire for cross } sectioning for subsequent nitrogen analysis by EPMA. } } Thanks! } john } } John J. Donovan donovan-at-uoregon.edu } University of Oregon (541) 346-4632 (office) } 1260 Franklin Blvd (541) 346-4655 (probe) } Eugene, OR (541) 346-4778 (SEM) } 97403-1272 (541) 346-4692 (FAX) } } Lab Web: http://epmalab.uoregon.edu/ } EPMA (SX100)Schedule: http://sweetwater.uoregon.edu/sx100 } EPMA (SX50) Schedule: http://sweetwater.uoregon.edu/epma } SEM (Ultra) Schedule: http://sweetwater.uoregon.edu/zeiss } SEM (Quanta) Schedule: http://sweetwater.uoregon.edu/sem } Remote Access: http://epmalab.uoregon.edu/howto.htm } Personal: http://www.uoregon.edu/~donovan/ } } "Aristotle presented with a moon rock would have no difficulty in } discerning it as an object not fundamentally different from } terrestrial materials. So much for Thomas Kuhn." } } "I'd rather be uncertain and wrong, than be certain and wrong." } } } } } ==============================Original Headers============================== } 9, 20 -- From donovan-at-uoregon.edu Thu Jan 25 11:24:37 2007 } 9, 20 -- Received: from smtp.uoregon.edu (mserv2.uoregon.edu [128.223.142.41]) } 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0PHOadg031693 } 9, 20 -- for {Microscopy-at-Microscopy.Com} ; Thu, 25 Jan 2007 11:24:36 -0600 } 9, 20 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.92.75]) } 9, 20 -- (authenticated bits=0) } 9, 20 -- by smtp.uoregon.edu (8.13.8/8.13.8) with ESMTP id l0PHOahW007001 } 9, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } 9, 20 -- for {Microscopy-at-Microscopy.Com} ; Thu, 25 Jan 2007 09:24:36 -0800 } 9, 20 -- Message-Id: {7.0.1.0.0.20070125092420.039399b8-at-uoregon.edu} } 9, 20 -- Message-Id: {7.0.1.0.0.20070125091904.038e7cd0-at-uoregon.edu} } 9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 } 9, 20 -- Date: Thu, 25 Jan 2007 09:24:32 -0800 } 9, 20 -- To: Microscopy-at-Microscopy.Com } 9, 20 -- From: John Donovan {donovan-at-uoregon.edu} } 9, 20 -- Subject: Nitrogen free epoxy? } 9, 20 -- Mime-Version: 1.0 } 9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } 9, 20 -- X-Virus-Scanned: ClamAV 0.88.7/2489/Thu Jan 25 04:35:24 2007 on mserv2 } 9, 20 -- X-Virus-Status: Clean } ==============================End of - Headers============================== }
==============================Original Headers============================== 13, 19 -- From dljones-at-bestweb.net Fri Jan 26 08:21:45 2007 13, 19 -- Received: from vms046pub.verizon.net (vms046pub.verizon.net [206.46.252.46]) 13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0QELjiD000634 13, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Jan 2007 08:21:45 -0600 13, 19 -- Received: from localhost ([71.247.249.221]) 13, 19 -- by vms046.mailsrvcs.net (Sun Java System Messaging Server 6.2-6.01 (built Apr 13, 19 -- 3 2006)) with ESMTPA id {0JCH00AC2BVXV1B7-at-vms046.mailsrvcs.net} for 13, 19 -- Microscopy-at-microscopy.com; Fri, 26 Jan 2007 08:21:39 -0600 (CST) 13, 19 -- Date: Fri, 26 Jan 2007 09:20:48 -0500 (Eastern Standard Time) 13, 19 -- From: "David L. Jones" {dljones-at-bestweb.net} 13, 19 -- Subject: Re: [Microscopy] Nitrogen free epoxy? 13, 19 -- In-reply-to: {200701251728.l0PHSolQ005552-at-ns.microscopy.com} 13, 19 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 13, 19 -- To: donovan-at-uoregon.edu 13, 19 -- Cc: Microscopy-at-microscopy.com 13, 19 -- Message-id: {Pine.WNT.4.64.0701260828200.4048-at-H-F1} 13, 19 -- MIME-version: 1.0 13, 19 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 13, 19 -- References: {200701251728.l0PHSolQ005552-at-ns.microscopy.com} ==============================End of - Headers==============================
We are planning to buy an ultramicrotome at a high probability, and we would like to microtome some materials for testing. If some labs in the manufacturers can help us, please contact me by email. Our materials are metal and perhaps ceramics.
Hiromi Konishi, Ph.D. Laboratory Manager The S.W. Bailey X-ray Diffraction Laboratory Room A353 Weeks Hall Voice: (608) 262-9784, (608) 262-0915 Fax: (608) 262-0693 Department of Geology and Geophysics University of Wisconsin-Madison 1215 W Dayton St. Madison WI 53706
==============================Original Headers============================== 6, 30 -- From hikonishi-at-gmail.com Fri Jan 26 12:35:31 2007 6, 30 -- Received: from py-out-1112.google.com (py-out-1112.google.com [64.233.166.178]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0QIZVU7018415 6, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Jan 2007 12:35:31 -0600 6, 30 -- Received: by py-out-1112.google.com with SMTP id b50so462752pyh 6, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Jan 2007 10:35:29 -0800 (PST) 6, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 30 -- d=gmail.com; s=beta; 6, 30 -- h=received:message-id:from:to:subject:date:mime-version:content-type:content-transfer-encoding:x-priority:x-msmail-priority:x-mailer:x-mimeole; 6, 30 -- b=IjEz3uvHL3yMtLR2aQgYhYeow1xdq1tuZWN/O3m2vm2bY4s2STDk26QWQYb5deLstS3b1VIuiWSX6CZLdqS57JePH8+w5Sz2HnT7kMIIEDxGrS++WwNcifsqytrVTfbhJXSW9MAG8DcA0cvztNFlkzcF7KBmV7EqV0U98x3iiJI= 6, 30 -- Received: by 10.35.96.11 with SMTP id y11mr4404012pyl.1169836529656; 6, 30 -- Fri, 26 Jan 2007 10:35:29 -0800 (PST) 6, 30 -- Received: from HKONISHI ( [144.92.207.106]) 6, 30 -- by mx.google.com with ESMTP id z52sm2803277pyg.2007.01.26.10.35.28; 6, 30 -- Fri, 26 Jan 2007 10:35:28 -0800 (PST) 6, 30 -- Message-ID: {000501c74178$bd28b0f0$6acf5c90-at-HKONISHI} 6, 30 -- From: "Hiromi Konishi" {hikonishi-at-gmail.com} 6, 30 -- To: {Microscopy-at-microscopy.com} 6, 30 -- Subject: Ultramicrotomy testing 6, 30 -- Date: Fri, 26 Jan 2007 12:35:05 -0600 6, 30 -- MIME-Version: 1.0 6, 30 -- Content-Type: text/plain; 6, 30 -- format=flowed; 6, 30 -- charset="iso-2022-jp"; 6, 30 -- reply-type=original 6, 30 -- Content-Transfer-Encoding: 7bit 6, 30 -- X-Priority: 3 6, 30 -- X-MSMail-Priority: Normal 6, 30 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 6, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
Hello list. I have a question that's just for fun. I'm using a Nikon TE-2000 wth all 4 ports and a beam splitter that can direct light 50/50 split between two ports. Is there a way to set up a camera to image not a sample but specifically the image that I'm seeing? I have a minor defect in my eye, a wrinkle caused by slight detachment of the vitreous (I think due to having LASIK done). When I look through the scope at a bright field, I can see an image of the wrinkle. I'm curious to know if I can capture it, but my guess is that the image only exists in the occulars? Maybe I could somehow use a dichroic mirror?
Thanks, Jessica
==============================Original Headers============================== 4, 37 -- From Jessica.Wagner-at-childrens.harvard.edu Fri Jan 26 14:24:29 2007 4, 37 -- Received: from mail2.childrenshospital.org (mail2.childrenshospital.org [134.174.20.64]) 4, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0QKOTAx003925 4, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Jan 2007 14:24:29 -0600 4, 37 -- Received: from tumsmtp1.CHBOSTON.ORG (tumsmtp1.tch.harvard.edu [10.1.101.46]) 4, 37 -- by mail2.childrenshospital.org (Postfix) with ESMTP id B770480F1 4, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Jan 2007 15:24:26 -0500 (EST) 4, 37 -- Received: from 10.1.102.175 by tumsmtp1.CHBOSTON.ORG with ESMTP (MMS 4, 37 -- SMTP Relay (Email Firewall v6.3.0)); Fri, 26 Jan 2007 15:24:13 -0500 4, 37 -- X-Server-Uuid: 1F337096-A893-456A-BE4C-C6341410F3EE 4, 37 -- Received: from CHEXV4.CHBOSTON.ORG ([10.1.102.188]) by 4, 37 -- chexsmtp2.CHBOSTON.ORG with Microsoft SMTPSVC(6.0.3790.1830); Fri, 26 4, 37 -- Jan 2007 15:24:13 -0500 4, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 4, 37 -- Content-class: urn:content-classes:message 4, 37 -- MIME-Version: 1.0 4, 37 -- Subject: imaging the occular view through another port? 4, 37 -- Date: Fri, 26 Jan 2007 15:24:12 -0500 4, 37 -- Message-ID: {6B335BE3804E0A40978750E51EFC076108B83B-at-CHEXV4.CHBOSTON.ORG} 4, 37 -- X-MS-Has-Attach: 4, 37 -- X-MS-TNEF-Correlator: 4, 37 -- Thread-Topic: imaging the occular view through another port? 4, 37 -- Thread-Index: AcdBh/FJTzzdmMO7TlybuNWpOytJvw== 4, 37 -- From: "Wagner, Jessica" {Jessica.Wagner-at-childrens.harvard.edu} 4, 37 -- To: Microscopy-at-microscopy.com 4, 37 -- X-OriginalArrivalTime: 26 Jan 2007 20:24:13.0614 (UTC) 4, 37 -- FILETIME=[F1C7C4E0:01C74187] 4, 37 -- X-TMWD-Spam-Summary: TS=20070126202415; SEV=2.2.0; DFV=B2007012608; 4, 37 -- IFV=2.0.4,4.0-9; AIF=B2007012608; RPD=5.02.0004; ENG=IBF; 4, 37 -- RPDID=7374723D303030312E30413031303230332E34354241363336462E303032442C73733D312C6667733D30; 4, 37 -- CAT=NONE; CON=NONE 4, 37 -- X-MMS-Spam-Filter-ID: B2007012608_5.02.0004_4.0-9 4, 37 -- X-WSS-ID: 69A4BCE73B47225184-01-01 4, 37 -- Content-Type: text/plain; 4, 37 -- charset=iso-8859-1 4, 37 -- Content-Transfer-Encoding: 8bit 4, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0QKOTAx003925 ==============================End of - Headers==============================
I have experienced this same phenomenon with "floaters" in my eyes. Under the right conditions, I can see the floaters beautifully and sometimes even the corneal surface. As explained to me by an ophthomologist, this is due to the projection of a shadow (of the objects) onto the retina. Therefore, you would not be able to "go the other way" and see the image in a viewing port. I believe that the only way to image this would be with a slit lamp (as in an ophthomological examination).
Maybe others on the list would care to comment since I am certainly no expert but speaking only from personal experience.
JB
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==============================Original Headers============================== 5, 20 -- From bozzola-at-siu.edu Fri Jan 26 15:01:39 2007 5, 20 -- Received: from abbmta1.siu.edu (abbmta1.siu.edu [131.230.254.205]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0QL1dYK003114 5, 20 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 26 Jan 2007 15:01:39 -0600 5, 20 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) 5, 20 -- by abbmta1.siu.edu (Switch-3.1.11/Switch-3.1.11) with ESMTP id l0QL15wG014885 5, 20 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 26 Jan 2007 15:01:16 -0600 (CST) 5, 20 -- Mime-Version: 1.0 5, 20 -- X-Sender: bozzola-at-saluki-mail.siu.edu 5, 20 -- Message-Id: {p06110401c1e01ae71b78-at-[131.230.177.142]} 5, 20 -- In-Reply-To: {200701262026.l0QKQHhM006040-at-ns.microscopy.com} 5, 20 -- References: {200701262026.l0QKQHhM006040-at-ns.microscopy.com} 5, 20 -- Date: Fri, 26 Jan 2007 15:01:04 -0600 5, 20 -- To: Microscopy-at-msa.microscopy.com 5, 20 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 5, 20 -- Subject: Re: [Microscopy] imaging the occular view through another port? 5, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 20 -- X-Spam-Score: 0.00% 5, 20 -- X-MASF: 0.00% 5, 20 -- X-Whitelist: 0.00% ==============================End of - Headers==============================
On Jan 26, 2007, at 12:24 PM, Jessica.Wagner-at-childrens.harvard.edu wrote:
} } Hello list. I have a question that's just for fun. I'm using a } } Nikon TE-2000 wth all 4 ports and a beam splitter that can direct } } light 50/50 split between two ports. Is there a way to set up a } } camera to image not a sample but specifically the image that I'm } } seeing? I have a minor defect in my eye, a wrinkle caused by slight } } detachment of the vitreous (I think due to having LASIK done). When } } I look through the scope at a bright field, I can see an image of the } } wrinkle. I'm curious to know if I can capture it, but my guess is } } that the image only exists in the occulars? Maybe I could somehow } } use a dichroic mirror? } } } I have experienced this same phenomenon with "floaters" in my eyes. } Under the right conditions, I can see the floaters beautifully and } sometimes even the corneal surface. As explained to me by an } ophthomologist, this is due to the projection of a shadow (of the } objects) onto the retina. Therefore, you would not be able to "go the } other way" and see the image in a viewing port. I believe that the } only way to image this would be with a slit lamp (as in an } ophthomological examination). } } Maybe others on the list would care to comment since I am certainly } no expert but speaking only from personal experience.
Dear Jessica and John, The image "that I'm seeing" can be thought of as the image that a user with normal sight would see transformed by the effect of the defect, so although it is easy to see the "normal" image, and as John suggests, you could see the defect with a slit lamp, you could not see the perturbation caused by the defect (although there are ways in theory to calculate it from the structure of the defect). Of course, you could look at a grid or some such test object and draw what you see, so the distortions noted in the grid will tell you what distortions to expect in a more complex image. You could even look at the grid separately with each eye and compare the image from the undamaged eye to that from the eye with the defect, and, if your brain has not already adapted to seeing the distorted image, looking at a grid that has been distorted in one eye could be interpreted by your brain as a 3D image of the grid that appears to be closer to you in some places than in others, like the effect of viewing stereo images, but much more subtle. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 5, 24 -- From tivol-at-caltech.edu Fri Jan 26 15:39:40 2007 5, 24 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0QLdexu017495 5, 24 -- for {microscopy-at-msa.microscopy.com} ; Fri, 26 Jan 2007 15:39:40 -0600 5, 24 -- Received: from water-dog.caltech.edu (water-dog [192.168.1.26]) 5, 24 -- by water-ox-postvirus (Postfix) with ESMTP id D8F352EF8F 5, 24 -- for {microscopy-at-msa.microscopy.com} ; Fri, 26 Jan 2007 13:39:35 -0800 (PST) 5, 24 -- Received: from [192.168.159.233] (jpix-01.caltech.edu [131.215.2.133]) 5, 24 -- (using TLSv1 with cipher RC4-SHA (128/128 bits)) 5, 24 -- (No client certificate requested) 5, 24 -- by earth-ox.its.caltech.edu (Postfix) with ESMTP id 9BF4810A109 5, 24 -- for {microscopy-at-msa.microscopy.com} ; Fri, 26 Jan 2007 13:39:34 -0800 (PST) 5, 24 -- Mime-Version: 1.0 (Apple Message framework v624) 5, 24 -- In-Reply-To: {200701262024.l0QKOc5u004144-at-ns.microscopy.com} 5, 24 -- References: {200701262024.l0QKOc5u004144-at-ns.microscopy.com} 5, 24 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 5, 24 -- Message-Id: {9a126178c30930f80e890b1ee25dc61d-at-caltech.edu} 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 24 -- Subject: Re: [Microscopy] imaging the occular view through another port? 5, 24 -- Date: Fri, 26 Jan 2007 13:47:32 -0800 5, 24 -- To: microscopy-at-msa.microscopy.com 5, 24 -- X-Mailer: Apple Mail (2.624) 5, 24 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.3.3 ==============================End of - Headers==============================
Unless you will be looking for trace amounts of nitrogen in your polymer, you might be able to get away with using one of the Epon epoxy substitutes, like EMbed 812.
Of its four components, only the accelerator DMP-30 has any N, 3 atoms per molecule, only a small amount is used in the mix, about 1.25% v/v, and the mass fraction of N in that 1.25% is even less. Another accelerator that can be used with EMbed812, BDMA, has only one N atom per molecule.
As it happened, I was scheduled to do some SEM-EDS this morning, so I took a look at a sample of an EMbed 812 block, hardened with DMP-30. I could see no detectable N peak in the spectra. I'll send you a JPG of a spectra off-List.
As you will be doing EPMA, your sensitivity for N maybe be greater. But you could just check some epoxy resins to see if there is enough N present to be a problem for your polymer work.
But of course, there is nothing sweeter than a nice, clean elemental analysis!
Good luck in your search for an N-free medium.
Gib
Gilbert (Gib) Ahlstrand, Electron Microscopist, Imaging Center, University of Minnesota 123 Snyder Hall St. Paul, MN 55108 http://www.cbs.umn.edu/ic
donovan-at-uoregon.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } All, } Can anyone tell me of a source for a nitrogen free epoxy? I realize } that may be a contradiction in terms, but I need to find a mounting } media that can hold a water soluble polymer coated wire for cross } sectioning for subsequent nitrogen analysis by EPMA. } } Thanks! } john } } John J. Donovan donovan-at-uoregon.edu } University of Oregon (541) 346-4632 (office) } 1260 Franklin Blvd (541) 346-4655 (probe) } Eugene, OR (541) 346-4778 (SEM) } 97403-1272 (541) 346-4692 (FAX)
==============================Original Headers============================== 10, 21 -- From ahlst007-at-umn.edu Fri Jan 26 15:46:29 2007 10, 21 -- Received: from mta-m2.tc.umn.edu (mta-m2.tc.umn.edu [160.94.23.21]) 10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0QLkTOO028495 10, 21 -- for {Microscopy-at-Microscopy.com} ; Fri, 26 Jan 2007 15:46:29 -0600 10, 21 -- Received: from [160.94.39.28] (x160-94-39-28.cbs.umn.edu [160.94.39.28]) 10, 21 -- by mta-m2.tc.umn.edu (UMN smtpd) with ESMTP 10, 21 -- for {Microscopy-at-Microscopy.com} ; Fri, 26 Jan 2007 15:46:28 -0600 (CST) 10, 21 -- X-Umn-Remote-Mta: [N] x160-94-39-28.cbs.umn.edu [160.94.39.28] #+LO+TS+AU+HN 10, 21 -- Message-ID: {45BA766B.7040308-at-umn.edu} 10, 21 -- Date: Fri, 26 Jan 2007 15:45:15 -0600 10, 21 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 10, 21 -- Reply-To: ahlst007-at-umn.edu 10, 21 -- Organization: Imaging Center UM 10, 21 -- User-Agent: Thunderbird 1.5.0.9 (Macintosh/20061207) 10, 21 -- MIME-Version: 1.0 10, 21 -- To: Microscopy-at-Microscopy.com 10, 21 -- Subject: Re: [Microscopy] Nitrogen free epoxy? 10, 21 -- References: {200701251728.l0PHSYC9005007-at-ns.microscopy.com} 10, 21 -- In-Reply-To: {200701251728.l0PHSYC9005007-at-ns.microscopy.com} 10, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
You probably would not like one possible approach to solving this imaging problem. I am thinking of Kevin Kline's solution to a similar problem in the movie "Wild Wild West".
Ralph Common
==============================Original Headers============================== 4, 24 -- From rcommon-at-msu.edu Fri Jan 26 15:58:38 2007 4, 24 -- Received: from sys28.mail.msu.edu (sys28.mail.msu.edu [35.9.75.128]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0QLwcbH007237 4, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Jan 2007 15:58:38 -0600 4, 24 -- Received: from [35.9.122.125] (helo=emlab) 4, 24 -- by sys28.mail.msu.edu with esmtpsa (Exim 4.52 #1) 4, 24 -- (TLSv1:RC4-MD5:128) 4, 24 -- id 1HAZ5i-00010e-9d 4, 24 -- for Microscopy-at-microscopy.com; Fri, 26 Jan 2007 16:58:38 -0500 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: imaging the occular view through another port? 4, 24 -- Date: Fri, 26 Jan 2007 17:00:10 -0500 4, 24 -- Message-ID: {006801c74195$5abe6c30$7d7a0923-at-msu.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="iso-8859-1" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 (Normal) 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1896 4, 24 -- Importance: Normal 4, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
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Title-Subject: [Filtered] Southeastern Microscopy Society annual meetin
Question: The 2007 annual meeting for the Southeastern Microscopy Society (SEMS) will be held April 11-13 in historic Decatur, Georgia, a suburb of Atlanta. We have an exciting program planned, including pre-meeting workshops on Bio-TEM Imaging, QuantomiX Wet SEM, Cryoultramicrotomy, and Deconvolution Microscopy. In addition, invited speakers will present information on such topics as the examination of the Gospel of Judas, World Trade Center dust, and macrophage cholesterol metabolism.
Deadlines for registration and abstract submission are February 23, 2007. There is additional meeting information available at the SEMS website at www.southeasternmicroscopy.org.
Cynthia S. Goldsmith Secretary, Southeastern Microscopy Society (SEMS)
I guess this is my month for desperate listserve queries, so here I go again.
We are trying to follow a not-very-detailed protocol for brain tissue which utilizes maleic acid buffer for wash steps before and after the "uranyl acetate colorization solution". One would presume that this refers to en bloc staining also using this buffer, but no details are given as to molarity, buffer composition, etc. We have no experience with this buffer, although I've heard of it, and are having a terrible time finding good references regarding its use and formulation.
My research in our reference library and online shows many instances of en bloc staining using maleic acid buffer, tris-maleate buffer, or simply maleic acid, so my questions are:
1) Are these terms pretty much interchangeable (i.e., is tris-maleate buffer just maleic acid buffer with the addition of tris)?
2) Do you all have any preferred formulations for this buffer in the UA step? Most of the refs I've seen use 0.05M - 0.2M buffer with 0.5-1% UA.
3) Is this buffer pretty much restricted to en bloc staining, at least for use in TEM studies?
4) What are the advantages of this buffer over aqueous en bloc staining with UA?
Thanks to all.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 12, 23 -- From TindallR-at-missouri.edu Mon Jan 29 09:38:23 2007 12, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0TFcNVY008362 12, 23 -- for {microscopy-at-microscopy.com} ; Mon, 29 Jan 2007 09:38:23 -0600 12, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 12, 23 -- Mon, 29 Jan 2007 09:38:23 -0600 12, 23 -- x-mimeole: Produced By Microsoft Exchange V6.5 12, 23 -- Content-class: urn:content-classes:message 12, 23 -- MIME-Version: 1.0 12, 23 -- Content-Type: text/plain; 12, 23 -- charset="us-ascii" 12, 23 -- Subject: TEM:Maleic vs. maleate vs. malic acid buffers 12, 23 -- Date: Mon, 29 Jan 2007 09:38:22 -0600 12, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68D7A-at-UM-XMAIL08.um.umsystem.edu} 12, 23 -- X-MS-Has-Attach: 12, 23 -- X-MS-TNEF-Correlator: 12, 23 -- Thread-Topic: TEM:Maleic vs. maleate vs. malic acid buffers 12, 23 -- Thread-Index: AcdDu4WL6a4MycTjQd+Res2DXMOOtQ== 12, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 12, 23 -- To: {microscopy-at-microscopy.com} 12, 23 -- X-OriginalArrivalTime: 29 Jan 2007 15:38:23.0068 (UTC) FILETIME=[827F61C0:01C743BB] 12, 23 -- Content-Transfer-Encoding: 8bit 12, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0TFcNVY008362 ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (mconstan-at-princeton.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, January 29, 2007 at 02:58:11 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both mconstan-at-princeton.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: mconstan-at-princeton.edu Name: Michael
Organization: Princeton University
Education: Undergraduate College
Location: Princeton, NJ, USA
Title: SEM beam penetration
Question: Hi. I'm an undergrad working in a lab and I would like to use SEM to view gold nanoparticles embedded in agarose gel. About how far could the SEM beam penetrate into 1% agarose (I'm guessing I would have to let the water in the gel evaporate)? Would it be better to use SE or BSE? What kV should I try? Thanks for any help you can give me!
Assume uranyl acetate stock is 5%. Dilute 1 part + 9 parts of 50 mM sodium maleate
En bloc staining protocol:
After osmium, rinse tissue 3x 5 min each in dH2O
rinse tissue 3x 5 min each in 50 mM sodium maleate, pH 5.2
incubate tissue for 1 hr to overnight in 0.5% - 1.0% uranyl acetate in 50 mM sodium maleate, pH 5.2 (for overnight, probably should use 0.5% UA, for 1 hr probably should use 1% UA).
rinse 3x dH2O for 10 min
50% ethanol 10 min
70% ethanol 10 min
95% ethanol 10 min
3x 100% ethanol 10 min
start resin infiltration
==============================Original Headers============================== 15, 19 -- From PhillipsT-at-missouri.edu Mon Jan 29 10:17:23 2007 15, 19 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 15, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0TGHMKd031221 15, 19 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 29 Jan 2007 10:17:23 -0600 15, 19 -- Received: from um-tsmtpout1.um.umsystem.edu ([209.106.228.23]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 15, 19 -- Mon, 29 Jan 2007 10:17:22 -0600 15, 19 -- Received: from phillips-dell.missouri.edu ([128.206.81.132]) by um-tsmtpout1.um.umsystem.edu over TLS secured channel with Microsoft SMTPSVC(6.0.3790.1830); 15, 19 -- Mon, 29 Jan 2007 10:17:21 -0600 15, 19 -- Message-Id: {6.0.0.22.2.20070129101755.04c27060-at-pop.missouri.edu} 15, 19 -- X-Sender: phillipst-at-pop.missouri.edu 15, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 6.0.0.22 15, 19 -- Date: Mon, 29 Jan 2007 10:19:18 -0600 15, 19 -- To: Microscopy-at-msa.microscopy.com 15, 19 -- From: Tom Phillips {phillipst-at-missouri.edu} 15, 19 -- Subject: Re: Maleate buffer 15, 19 -- Cc: tindallr-at-missouri.edu 15, 19 -- Mime-Version: 1.0 15, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 15, 19 -- X-OriginalArrivalTime: 29 Jan 2007 16:17:22.0173 (UTC) FILETIME=[F4B67AD0:01C743C0] ==============================End of - Headers==============================
What kind of detail do you need? That will probably determine your conditions.
Understand that the beam will start scattering and interacting once it encounters the first surface which will be agarose. Secondary electrons are limited to escaping from the first few nm of surface, so even if they are generated at some depth, they will not escape to be detected. I expect you would see primarily the agarose surface.
There is also the question of imaging the agarose at all. I presume it would charge without coating and would need to be examined in variable pressure or environmental mode. That would eliminate the choice of SE unless you have a gaseous SE detector, in which case the previous comments still apply.
BSE could give you some information from some depth below the surface. It will still be difficult and there will be some scattering.
Also, what happens to the structure of the agarose under vacuum? I suppose there will be appreciable loss of moisture, so what you see may not be anything like what you had. I would check the mass loss as a result of exposure to vacuum. It might be too extreme.
I would probably start at 20kV for voltage and set the current to give a decent BSE image. You will probably have to experiment from there.
Warren Straszheim
-----Original Message----- X-from: mconstan-at-princeton.edu [mailto:mconstan-at-princeton.edu] Sent: Monday, January 29, 2007 10:05 AM To: wesaia-at-iastate.edu
Jessica; Some time ago, there was a thread about LASIK and microscopists. I do not recall any of the reports mentioning this sort of post-surgical vision difficulty. Is this perhaps a subject that should be re-visited?
John Mardinly Intel
Disclaimer: The opinions of this author do not represent the opinions of Intel Corporation.
-----Original Message----- X-from: Jessica.Wagner-at-childrens.harvard.edu [mailto:Jessica.Wagner-at-childrens.harvard.edu] Sent: Friday, January 26, 2007 12:25 PM To: Mardinly, John
Hello list. I have a question that's just for fun. I'm using a Nikon TE-2000 wth all 4 ports and a beam splitter that can direct light 50/50 split between two ports. Is there a way to set up a camera to image not a sample but specifically the image that I'm seeing? I have a minor defect in my eye, a wrinkle caused by slight detachment of the vitreous (I think due to having LASIK done). When I look through the scope at a bright field, I can see an image of the wrinkle. I'm curious to know if I can capture it, but my guess is that the image only exists in the occulars? Maybe I could somehow use a dichroic mirror?
Thanks, Jessica
==============================Original Headers============================== 4, 37 -- From Jessica.Wagner-at-childrens.harvard.edu Fri Jan 26 14:24:29 2007 4, 37 -- Received: from mail2.childrenshospital.org (mail2.childrenshospital.org [134.174.20.64]) 4, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0QKOTAx003925 4, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Jan 2007 14:24:29 -0600 4, 37 -- Received: from tumsmtp1.CHBOSTON.ORG (tumsmtp1.tch.harvard.edu [10.1.101.46]) 4, 37 -- by mail2.childrenshospital.org (Postfix) with ESMTP id B770480F1 4, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Jan 2007 15:24:26 -0500 (EST) 4, 37 -- Received: from 10.1.102.175 by tumsmtp1.CHBOSTON.ORG with ESMTP (MMS 4, 37 -- SMTP Relay (Email Firewall v6.3.0)); Fri, 26 Jan 2007 15:24:13 -0500 4, 37 -- X-Server-Uuid: 1F337096-A893-456A-BE4C-C6341410F3EE 4, 37 -- Received: from CHEXV4.CHBOSTON.ORG ([10.1.102.188]) by 4, 37 -- chexsmtp2.CHBOSTON.ORG with Microsoft SMTPSVC(6.0.3790.1830); Fri, 26 4, 37 -- Jan 2007 15:24:13 -0500 4, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 4, 37 -- Content-class: urn:content-classes:message 4, 37 -- MIME-Version: 1.0 4, 37 -- Subject: imaging the occular view through another port? 4, 37 -- Date: Fri, 26 Jan 2007 15:24:12 -0500 4, 37 -- Message-ID: {6B335BE3804E0A40978750E51EFC076108B83B-at-CHEXV4.CHBOSTON.ORG} 4, 37 -- X-MS-Has-Attach: 4, 37 -- X-MS-TNEF-Correlator: 4, 37 -- Thread-Topic: imaging the occular view through another port? 4, 37 -- Thread-Index: AcdBh/FJTzzdmMO7TlybuNWpOytJvw== 4, 37 -- From: "Wagner, Jessica" {Jessica.Wagner-at-childrens.harvard.edu} 4, 37 -- To: Microscopy-at-microscopy.com 4, 37 -- X-OriginalArrivalTime: 26 Jan 2007 20:24:13.0614 (UTC) 4, 37 -- FILETIME=[F1C7C4E0:01C74187] 4, 37 -- X-TMWD-Spam-Summary: TS=20070126202415; SEV=2.2.0; DFV=B2007012608; 4, 37 -- IFV=2.0.4,4.0-9; AIF=B2007012608; RPD=5.02.0004; ENG=IBF; 4, 37 -- RPDID=7374723D303030312E30413031303230332E34354241363336462E303032442C73 733D312C6667733D30; 4, 37 -- CAT=NONE; CON=NONE 4, 37 -- X-MMS-Spam-Filter-ID: B2007012608_5.02.0004_4.0-9 4, 37 -- X-WSS-ID: 69A4BCE73B47225184-01-01 4, 37 -- Content-Type: text/plain; 4, 37 -- charset=iso-8859-1 4, 37 -- Content-Transfer-Encoding: 8bit 4, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0QKOTAx003925 ==============================End of - Headers==============================
==============================Original Headers============================== 13, 34 -- From john.mardinly-at-intel.com Mon Jan 29 11:51:39 2007 13, 34 -- Received: from mga02.intel.com (mga02.intel.com [134.134.136.20]) 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0THpd49013923 13, 34 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 29 Jan 2007 11:51:39 -0600 13, 34 -- Received: from orsmga001.jf.intel.com ([10.7.209.18]) 13, 34 -- by mga02.intel.com with ESMTP; 29 Jan 2007 09:51:39 -0800 13, 34 -- Received: from fmsmsx333.amr.corp.intel.com ([132.233.42.2]) 13, 34 -- by orsmga001.jf.intel.com with ESMTP; 29 Jan 2007 09:51:38 -0800 13, 34 -- X-ExtLoop1: 1 13, 34 -- X-IronPort-AV: i="4.13,253,1167638400"; 13, 34 -- d="scan'208"; a="190293831:sNHT77980763" 13, 34 -- Received: from scsmsx411.amr.corp.intel.com ([10.3.90.30]) by fmsmsx333.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 13, 34 -- Mon, 29 Jan 2007 09:51:31 -0800 13, 34 -- Received: from scsmsx413.amr.corp.intel.com ([10.3.90.32]) by scsmsx411.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 13, 34 -- Mon, 29 Jan 2007 09:51:30 -0800 13, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 13, 34 -- Content-class: urn:content-classes:message 13, 34 -- MIME-Version: 1.0 13, 34 -- Content-Type: text/plain; 13, 34 -- charset="us-ascii" 13, 34 -- Subject: RE: [Microscopy] imaging the occular view through another port? 13, 34 -- Date: Mon, 29 Jan 2007 09:51:29 -0800 13, 34 -- Message-ID: {1DF4C4D62339DB4C9C98DF04213995B60263F0B5-at-scsmsx413.amr.corp.intel.com} 13, 34 -- In-Reply-To: {200701262024.l0QKObkm004099-at-ns.microscopy.com} 13, 34 -- X-MS-Has-Attach: 13, 34 -- X-MS-TNEF-Correlator: 13, 34 -- Thread-Topic: [Microscopy] imaging the occular view through another port? 13, 34 -- Thread-Index: AcdBiAPf8S8hIZO1T+u/pIAfVESIzACRKOmg 13, 34 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 13, 34 -- To: {Jessica.Wagner-at-childrens.harvard.edu} 13, 34 -- Cc: {Microscopy-at-msa.microscopy.com} 13, 34 -- X-OriginalArrivalTime: 29 Jan 2007 17:51:30.0799 (UTC) FILETIME=[1B8E67F0:01C743CE] 13, 34 -- Content-Transfer-Encoding: 8bit 13, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0THpd49013923 ==============================End of - Headers==============================
Another possibility is to use a mounting material from long,long ago. This is Wood's metal, a quaternary eutectic of Bi/50%, Pb/25%, Sn12.5%, Cd12.5%. It's used for sprinkler plugs in automatic systems. If your polymer can stand 70'C, (melting point of the alloy) it.s a good way to go since you be sure there's no nitrogen in it. The alloy is fairly soft and it wet's well.
Vern Griffiths Montana Tech
==============================Original Headers============================== 3, 23 -- From VGriffiths-at-mtech.edu Mon Jan 29 12:00:34 2007 3, 23 -- Received: from cug1.umt.edu (cug1.umt.edu [150.131.192.195]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0TI0XPf001959 3, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 29 Jan 2007 12:00:33 -0600 3, 23 -- Received: from mtmailr3.butte.campus (mtmailr3.mtech.edu [10.34.34.114]) 3, 23 -- by cug1.umt.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l0TI0WJh012580 3, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 29 Jan 2007 11:00:32 -0700 (MST) 3, 23 -- Content-class: urn:content-classes:message 3, 23 -- MIME-Version: 1.0 3, 23 -- Content-Type: text/plain; 3, 23 -- charset="us-ascii" 3, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 3, 23 -- Subject: Epoxy free mounting material 3, 23 -- Date: Mon, 29 Jan 2007 11:00:32 -0700 3, 23 -- Message-ID: {137E940998125E4FBEE1F53EBA11D800E5BB5E-at-mtmailr3.butte.campus} 3, 23 -- X-MS-Has-Attach: 3, 23 -- X-MS-TNEF-Correlator: 3, 23 -- Thread-Topic: Epoxy free mounting material 3, 23 -- Thread-Index: AcdDz15U04uodbytS1WUWB3KRqeNbw== 3, 23 -- From: "Griffiths, Vern" {VGriffiths-at-mtech.edu} 3, 23 -- To: {Microscopy-at-microscopy.com} 3, 23 -- Content-Transfer-Encoding: 8bit 3, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0TI0XPf001959 ==============================End of - Headers==============================
I'm teaching a bioimaging course for undergraduates. We are now discussing the lens equations that relate object and image distance to focal length:
1/p + 1/q = 1/f
and magnification:
M = q/p
where p is the object distance and q is the image distance.
We've demonstrated these relationships using lenses on an optical bench, and now I want to transfer to real microscopes.
The students have access to Nikon Alphaphot microscopes with a fixed tube length of 160 mm, which I understand is measured from the nosepiece to the eyepiece mount. The eyepiece mount is approximately the same as the primary image plane on these 'scopes. So does that mean that the image distance for each lens should be 160 mm plus the distance to the middle of the objective lens? Or is it more complicated than that, because the lens equations don't work out very well using those values for image distance.
For example, the 10x objective should then have an image distance of about 160 + 38 mm = 198 mm. Which would then predict an object distance of 19.8 mm and a focal length of 18 mm. All of that makes sense since the object should be just beyond the front focal point of the objective to make a real inverted image at the primary image plane.
But in practice, the working distance of this lens is 6.1 mm, which puts the actual object distance at around 6.3 mm, well inside the presumed focal point, and puts the image distance at around 63 mm for a 10 magnification
Something doesn't add up. What am I missing?
Gary P. Radice gradice-at-richmond.edu Department of Biology 804-289-8107 (voice) University of Richmond 804-289-8233 (FAX) Richmond VA 23173 http://www.richmond.edu/~gradice
==============================Original Headers============================== 17, 21 -- From gradice-at-richmond.edu Mon Jan 29 14:05:38 2007 17, 21 -- Received: from spandex.richmond.edu (spandex.richmond.edu [141.166.24.58]) 17, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0TK5XPQ015998 17, 21 -- for {microscopy-at-microscopy.com} ; Mon, 29 Jan 2007 14:05:36 -0600 17, 21 -- Received: from rayon.richmond.edu (rayon.richmond.edu [141.166.30.10]) 17, 21 -- by spandex.richmond.edu (8.13.1/8.13.1) with ESMTP id l0TK5WbO021856 17, 21 -- for {microscopy-at-microscopy.com} ; Mon, 29 Jan 2007 15:05:32 -0500 17, 21 -- Received: from [141.166.185.175] (ffa185175.richmond.edu [141.166.185.175]) 17, 21 -- by rayon.richmond.edu (8.12.11.20060308/8.12.11) with ESMTP id l0TK5WUW007000 17, 21 -- for {microscopy-at-microscopy.com} ; Mon, 29 Jan 2007 15:05:32 -0500 17, 21 -- Mime-Version: 1.0 (Apple Message framework v752.2) 17, 21 -- Content-Transfer-Encoding: 7bit 17, 21 -- Message-Id: {38ED7F44-618C-4970-9B9E-C5BC26FAE970-at-richmond.edu} 17, 21 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 17, 21 -- To: microscopy-at-microscopy.com 17, 21 -- From: gradice {gradice-at-richmond.edu} 17, 21 -- Subject: real microscopes vs lens equations 17, 21 -- Date: Mon, 29 Jan 2007 15:04:25 -0500 17, 21 -- X-Mailer: Apple Mail (2.752.2) 17, 21 -- X-Spam-Detail: -1.44 ALL_TRUSTED 17, 21 -- X-Scanned-By: MIMEDefang 2.49 on 141.166.30.10 ==============================End of - Headers==============================
I missed that thread, but I'll be happy to add my two cents now. Assuming the PVD (posterior vitreous detachment) doesn't get any worse, for me the benefits of LASIK still outweigh this minor negative. The 'floater' that I see is an annoyance, but it doesn't truly inhibit my vision through the scope (or outside the scope for that matter).
But I had LASIK only a year ago, and was never warned PVD could be a complication, so this says to me that there is still much unknown about the procedure and it's results. Unfortunately, complications of laser eye surgeries really aren't tracked all that well; doctors are not required to report them, unless they are related to a device, but even then, many doctors are not aware of FDA regulations about device event reporting or how/to whom they should report adverse events.
Here is an article about PVD's and LASIK: http://www.springerlink.com/content/j3100858467pu5k1/
And thanks to everyone who offered imaging advice!
Jessica
-----Original Message----- X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com] Sent: Monday, January 29, 2007 12:56 PM To: Wagner, Jessica
Jessica; Some time ago, there was a thread about LASIK and microscopists. I do not recall any of the reports mentioning this sort of post-surgical vision difficulty. Is this perhaps a subject that should be re-visited?
John Mardinly Intel
Disclaimer: The opinions of this author do not represent the opinions of Intel Corporation.
-----Original Message----- X-from: Jessica.Wagner-at-childrens.harvard.edu [mailto:Jessica.Wagner-at-childrens.harvard.edu] Sent: Friday, January 26, 2007 12:25 PM To: Mardinly, John
Hello list. I have a question that's just for fun. I'm using a Nikon TE-2000 wth all 4 ports and a beam splitter that can direct light 50/50 split between two ports. Is there a way to set up a camera to image not a sample but specifically the image that I'm seeing? I have a minor defect in my eye, a wrinkle caused by slight detachment of the vitreous (I think due to having LASIK done). When I look through the scope at a bright field, I can see an image of the wrinkle. I'm curious to know if I can capture it, but my guess is that the image only exists in the occulars? Maybe I could somehow use a dichroic mirror?
Thanks, Jessica
==============================Original Headers============================== 4, 37 -- From Jessica.Wagner-at-childrens.harvard.edu Fri Jan 26 14:24:29 2007 4, 37 -- Received: from mail2.childrenshospital.org (mail2.childrenshospital.org [134.174.20.64]) 4, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0QKOTAx003925 4, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Jan 2007 14:24:29 -0600 4, 37 -- Received: from tumsmtp1.CHBOSTON.ORG (tumsmtp1.tch.harvard.edu [10.1.101.46]) 4, 37 -- by mail2.childrenshospital.org (Postfix) with ESMTP id B770480F1 4, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Jan 2007 15:24:26 -0500 (EST) 4, 37 -- Received: from 10.1.102.175 by tumsmtp1.CHBOSTON.ORG with ESMTP (MMS 4, 37 -- SMTP Relay (Email Firewall v6.3.0)); Fri, 26 Jan 2007 15:24:13 -0500 4, 37 -- X-Server-Uuid: 1F337096-A893-456A-BE4C-C6341410F3EE 4, 37 -- Received: from CHEXV4.CHBOSTON.ORG ([10.1.102.188]) by 4, 37 -- chexsmtp2.CHBOSTON.ORG with Microsoft SMTPSVC(6.0.3790.1830); Fri, 26 4, 37 -- Jan 2007 15:24:13 -0500 4, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 4, 37 -- Content-class: urn:content-classes:message 4, 37 -- MIME-Version: 1.0 4, 37 -- Subject: imaging the occular view through another port? 4, 37 -- Date: Fri, 26 Jan 2007 15:24:12 -0500 4, 37 -- Message-ID: {6B335BE3804E0A40978750E51EFC076108B83B-at-CHEXV4.CHBOSTON.ORG} 4, 37 -- X-MS-Has-Attach: 4, 37 -- X-MS-TNEF-Correlator: 4, 37 -- Thread-Topic: imaging the occular view through another port? 4, 37 -- Thread-Index: AcdBh/FJTzzdmMO7TlybuNWpOytJvw== 4, 37 -- From: "Wagner, Jessica" {Jessica.Wagner-at-childrens.harvard.edu} 4, 37 -- To: Microscopy-at-microscopy.com 4, 37 -- X-OriginalArrivalTime: 26 Jan 2007 20:24:13.0614 (UTC) 4, 37 -- FILETIME=[F1C7C4E0:01C74187] 4, 37 -- X-TMWD-Spam-Summary: TS=20070126202415; SEV=2.2.0; DFV=B2007012608; 4, 37 -- IFV=2.0.4,4.0-9; AIF=B2007012608; RPD=5.02.0004; ENG=IBF; 4, 37 -- RPDID=7374723D303030312E30413031303230332E34354241363336462E303032442C73 733D312C6667733D30; 4, 37 -- CAT=NONE; CON=NONE 4, 37 -- X-MMS-Spam-Filter-ID: B2007012608_5.02.0004_4.0-9 4, 37 -- X-WSS-ID: 69A4BCE73B47225184-01-01 4, 37 -- Content-Type: text/plain; 4, 37 -- charset=iso-8859-1 4, 37 -- Content-Transfer-Encoding: 8bit 4, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0QKOTAx003925 ==============================End of - Headers==============================
==============================Original Headers============================== 13, 34 -- From john.mardinly-at-intel.com Mon Jan 29 11:51:39 2007 13, 34 -- Received: from mga02.intel.com (mga02.intel.com [134.134.136.20]) 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0THpd49013923 13, 34 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 29 Jan 2007 11:51:39 -0600 13, 34 -- Received: from orsmga001.jf.intel.com ([10.7.209.18]) 13, 34 -- by mga02.intel.com with ESMTP; 29 Jan 2007 09:51:39 -0800 13, 34 -- Received: from fmsmsx333.amr.corp.intel.com ([132.233.42.2]) 13, 34 -- by orsmga001.jf.intel.com with ESMTP; 29 Jan 2007 09:51:38 -0800 13, 34 -- X-ExtLoop1: 1 13, 34 -- X-IronPort-AV: i="4.13,253,1167638400"; 13, 34 -- d="scan'208"; a="190293831:sNHT77980763" 13, 34 -- Received: from scsmsx411.amr.corp.intel.com ([10.3.90.30]) by fmsmsx333.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 13, 34 -- Mon, 29 Jan 2007 09:51:31 -0800 13, 34 -- Received: from scsmsx413.amr.corp.intel.com ([10.3.90.32]) by scsmsx411.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 13, 34 -- Mon, 29 Jan 2007 09:51:30 -0800 13, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 13, 34 -- Content-class: urn:content-classes:message 13, 34 -- MIME-Version: 1.0 13, 34 -- Content-Type: text/plain; 13, 34 -- charset="us-ascii" 13, 34 -- Subject: RE: [Microscopy] imaging the occular view through another port? 13, 34 -- Date: Mon, 29 Jan 2007 09:51:29 -0800 13, 34 -- Message-ID: {1DF4C4D62339DB4C9C98DF04213995B60263F0B5-at-scsmsx413.amr.corp.intel.com} 13, 34 -- In-Reply-To: {200701262024.l0QKObkm004099-at-ns.microscopy.com} 13, 34 -- X-MS-Has-Attach: 13, 34 -- X-MS-TNEF-Correlator: 13, 34 -- Thread-Topic: [Microscopy] imaging the occular view through another port? 13, 34 -- Thread-Index: AcdBiAPf8S8hIZO1T+u/pIAfVESIzACRKOmg 13, 34 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 13, 34 -- To: {Jessica.Wagner-at-childrens.harvard.edu} 13, 34 -- Cc: {Microscopy-at-msa.microscopy.com} 13, 34 -- X-OriginalArrivalTime: 29 Jan 2007 17:51:30.0799 (UTC) FILETIME=[1B8E67F0:01C743CE] 13, 34 -- Content-Transfer-Encoding: 8bit 13, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0THpd49013923 ==============================End of - Headers==============================
==============================Original Headers============================== 28, 37 -- From Jessica.Wagner-at-childrens.harvard.edu Mon Jan 29 16:18:54 2007 28, 37 -- Received: from mail2.childrenshospital.org (mail2.childrenshospital.org [134.174.20.64]) 28, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0TMIrbi030608 28, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 29 Jan 2007 16:18:53 -0600 28, 37 -- Received: from tumsmtp1.CHBOSTON.ORG (tumsmtp1.tch.harvard.edu [10.1.101.46]) 28, 37 -- by mail2.childrenshospital.org (Postfix) with ESMTP id C3D458082 28, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 29 Jan 2007 17:18:50 -0500 (EST) 28, 37 -- Received: from 10.1.102.174 by tumsmtp1.CHBOSTON.ORG with ESMTP (MMS 28, 37 -- SMTP Relay (Email Firewall v6.3.0)); Mon, 29 Jan 2007 17:18:43 -0500 28, 37 -- X-Server-Uuid: 1F337096-A893-456A-BE4C-C6341410F3EE 28, 37 -- Received: from CHEXV4.CHBOSTON.ORG ([10.1.102.188]) by 28, 37 -- chexsmtp1.CHBOSTON.ORG with Microsoft SMTPSVC(6.0.3790.1830); Mon, 29 28, 37 -- Jan 2007 17:18:43 -0500 28, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 28, 37 -- Content-class: urn:content-classes:message 28, 37 -- MIME-Version: 1.0 28, 37 -- Subject: LASIK and microscopists 28, 37 -- Date: Mon, 29 Jan 2007 17:18:42 -0500 28, 37 -- Message-ID: {6B335BE3804E0A40978750E51EFC076107BF91-at-CHEXV4.CHBOSTON.ORG} 28, 37 -- X-MS-Has-Attach: 28, 37 -- X-MS-TNEF-Correlator: 28, 37 -- Thread-Topic: LASIK and microscopists 28, 37 -- Thread-Index: AcdD829TWE3SHiDVT4OxJPpkd6QQAg== 28, 37 -- From: "Wagner, Jessica" {Jessica.Wagner-at-childrens.harvard.edu} 28, 37 -- To: Microscopy-at-microscopy.com 28, 37 -- X-OriginalArrivalTime: 29 Jan 2007 22:18:43.0039 (UTC) 28, 37 -- FILETIME=[6F8416F0:01C743F3] 28, 37 -- X-TMWD-Spam-Summary: TS=20070129221845; SEV=2.2.0; DFV=B2007012910; 28, 37 -- IFV=2.0.4,4.0-9; AIF=B2007012910; RPD=5.02.0004; ENG=IBF; 28, 37 -- RPDID=7374723D303030312E30413031303230312E34354245373243342E303046372C73733D312C6667733D30; 28, 37 -- CAT=NONE; CON=NONE 28, 37 -- X-MMS-Spam-Filter-ID: B2007012910_5.02.0004_4.0-9 28, 37 -- X-WSS-ID: 69A0AD493B47707430-01-01 28, 37 -- Content-Type: text/plain; 28, 37 -- charset=us-ascii 28, 37 -- Content-Transfer-Encoding: 8bit 28, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0TMIrbi030608 ==============================End of - Headers==============================
One of my users is trying to visualize virus particles by negative stain, normally a routine procedure here. However, her sample is straight off a cesium chloride purification. Will the CsCl solution inhibit binding of the virus particles to coated grids, or should she be able to use standard neg. staining procedures to see her particles. She didn't see any particles in her first attempts. While trying to calculate virus concentrations to determine if her sample is concentrated enough, I wondered if the CsCl will inhibit virus sticking to the grids--I have no experience with virus samples that have not been purified into some other buffer system.
Anyone have a protocol we can use for such a sample, or does she need to get rid of the CsCl before she tries to do any negative stain imaging.
Your advice is appreciated.
Steve -- Dr. Steven Barlow EM Facility/Biology Dept. San Diego State University 5500 Campanile Drive San Diego CA 92182-4614 phone: (619) 594-4523 fax: (619) 594-5676
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Question: ...Try#2: Prior attempt lost line-breaks... {br /}
With light, fluorescence or confocal microscopy experience, a microscopy specialist is sought to provide user support in Johns Hopkins School of Medicine Microscope Facility (http://www.hopkinsmedicine.org/micfac/). This facility provides light, fluorescence and electron microscopy services to more than 250 users throughout Johns Hopkins. As part of a team with other full-time staff, the candidateís main duties are to help assist in training and supervising new users, as well as help users troubleshoot experiments. Because of the diversity of equipment within the facility, we will provide initial training as necessary. Consequently, the candidate must display resourceful independence, a willingness to learn and have strong analytical and computer skills. {br /}
Please refer to JHUjobs (http://jobs.jhu.edu/) posting #26703 (https://hrnt.jhu.edu/jhujobs/job_view.cfm?view_req_id=26703) for details about minimal qualifications and application process. More qualified individuals will be considered for appropriate compensation. {br /}
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Email: swtkeller-at-yahoo.com Name: Sandra Keller
Organization: TA/SICCO
Title-Subject: [Filtered] TEM-Defect density Threshold
Question: Hi All: I was asked by a colleague of mine to inquire from the Listserver: What is the approximate defect density in silicon, one can easily visualize using a 120-200 KV TEM (plane view and cross-sectional view)?
Thanks in advance for being such a valuable resource, Sandra Keller
This Question was submitted to Ask-A-Microscopist by (mconstan-at-princeton.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, January 29, 2007 at 02:58:11 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both mconstan-at-princeton.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: mconstan-at-princeton.edu Name: Michael
Organization: Princeton University
Education: Undergraduate College
Location: Princeton, NJ, USA
Title: SEM beam penetration
Question: Hi. I'm an undergrad working in a lab and I would like to use SEM to view gold nanoparticles embedded in agarose gel. About how far could the SEM beam penetrate into 1% agarose (I'm guessing I would have to let the water in the gel evaporate)? Would it be better to use SE or BSE? What kV should I try? Thanks for any help you can give me!
Hi Sandra, It's an easy calculation to do if you make some rough assumptions about the amount of thin material in your TEM specimen and the visibility of defects. For a standard cross section which has roughly 100um of electron transparent material away from the edges of the hole and is on average 1um thick, you have 0.01 x 0.0001 = 0.000001 cm^2 of the original wafer surface in your cross-section specimen. So if you see just one defect you have a density of 10^6 defects cm^-2. If your defects are only visible in thin material then revise the number upwards. If you have a FIB section (say 30um wide and 0.3 um thick) you need over 10^8 defects cm^-2 to catch one in your specimen.
For a plan view specimen which has the same 100um electron transparent area and average thickness of 1um, say around a hole 50um wide then the amount of the original wafer surface you can see is pi*(150um)^2-pi*(50um)^2 = 0.0006 cm^2. So if you see one defect you have a density of about 1600 defects cm^-2.
Having said that I know from experience it can take some time to find defects in plan view specimens even when the density as high as 10^5 defects cm^-2, simply because the specimen is always bent and you have to tilt the specimen through a diffraction condition to see them.
Defect etching is very useful for seeing low densities of defects if processing allows. You can even do a light defect etch and then make a TEM specimen - in which case you will find that not all of the defects actually produce an etch pit, which just goes to show that there is no perfect technique for measuring defect densities.
-----Original Message----- X-from: swtkeller-at-yahoo.com [mailto:swtkeller-at-yahoo.com] Sent: 30 January 2007 03:08 To: Richard Beanland
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both swtkeller-at-yahoo.com as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: swtkeller-at-yahoo.com Name: Sandra Keller
Organization: TA/SICCO
Title-Subject: [Filtered] TEM-Defect density Threshold
Question: Hi All: I was asked by a colleague of mine to inquire from the Listserver: What is the approximate defect density in silicon, one can easily visualize using a 120-200 KV TEM (plane view and cross-sectional view)?
Thanks in advance for being such a valuable resource, Sandra Keller
==============================Original Headers============================== 6, 12 -- From zaluzec-at-microscopy.com Mon Jan 29 21:06:45 2007 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0U36i9C026687 6, 12 -- for {microscopy-at-microscopy.com} ; Mon, 29 Jan 2007 21:06:44 -0600 6, 12 -- Mime-Version: 1.0 6, 12 -- X-Sender: (Unverified) 6, 12 -- Message-Id: {p06110401c1e466b52e6d-at-[206.69.208.22]} 6, 12 -- Date: Mon, 29 Jan 2007 21:06:42 -0600 6, 12 -- To: microscopy-at-microscopy.com 6, 12 -- From: swtkeller-at-yahoo.com (by way of MicroscopyListserver) 6, 12 -- Subject: viaWWW: TEM-Defect density Threshold 6, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
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==============================Original Headers============================== 22, 32 -- From richard.beanland-at-bookham.com Tue Jan 30 03:10:23 2007 22, 32 -- Received: from mail82.messagelabs.com (mail82.messagelabs.com [195.245.231.67]) 22, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0U9AMfR031960 22, 32 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 03:10:22 -0600 22, 32 -- X-VirusChecked: Checked 22, 32 -- X-Env-Sender: richard.beanland-at-bookham.com 22, 32 -- X-Msg-Ref: server-6.tower-82.messagelabs.com!1170148220!1610727!3 22, 32 -- X-StarScan-Version: 5.5.10.7.1; banners=bookham.com,-,- 22, 32 -- X-Originating-IP: [213.249.209.179] 22, 32 -- Received: (qmail 23692 invoked from network); 30 Jan 2007 09:10:20 -0000 22, 32 -- Received: from unknown (HELO cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 22, 32 -- by server-6.tower-82.messagelabs.com with SMTP; 30 Jan 2007 09:10:20 -0000 22, 32 -- Received: from cas-smx-02.BOOKHAM.ENTERPRISE.PRI ([10.4.0.223]) by cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI with Microsoft SMTPSVC(6.0.3790.1830); 22, 32 -- Tue, 30 Jan 2007 09:11:38 +0000 22, 32 -- Content-class: urn:content-classes:message 22, 32 -- MIME-Version: 1.0 22, 32 -- Content-Type: text/plain; 22, 32 -- charset="us-ascii" 22, 32 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 22, 32 -- Subject: RE: [Microscopy] viaWWW: TEM-Defect density Threshold 22, 32 -- Date: Tue, 30 Jan 2007 09:11:35 -0000 22, 32 -- Message-ID: {9645D3E33E4C6548B12A7B25F611533E353709-at-cas-smx-02.BOOKHAM.ENTERPRISE.PRI} 22, 32 -- X-MS-Has-Attach: 22, 32 -- X-MS-TNEF-Correlator: 22, 32 -- Thread-Topic: [Microscopy] viaWWW: TEM-Defect density Threshold 22, 32 -- Thread-Index: AcdEG/1pUaZIhLZCRG2NwtjBllRCOwAL5JIw 22, 32 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 22, 32 -- To: {swtkeller-at-yahoo.com} 22, 32 -- Cc: {microscopy-at-microscopy.com} 22, 32 -- X-OriginalArrivalTime: 30 Jan 2007 09:11:38.0341 (UTC) FILETIME=[A5D0C950:01C7444E] 22, 32 -- Content-Transfer-Encoding: 8bit 22, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0U9AMfR031960 ==============================End of - Headers==============================
The simplest solution to your problem is to down load one of the basic Monte Carlo simulations and consider your material to be Carbon. The Joy-Nockolds version is on our web site under hints and tips.
Depending upon the microscope that you are using, the magnification, the kV and the working distance, you could find the so called "secondary electron signal" (everhart-Thornley detector) carries sufficient backscattered information to provide the data that you require. Be aware of the fall off in resolution when using dedicated backscatter as the performance may fall below that required to visualise your particles. You should also consider that depending on the microscope and your own skill the size of particles may be too small to visualise?
You should try the complete range of kV as the information will always be better at one particular kV. It is impossible perhaps foolish to try and guess what that may be. You may need higher kVs for penetration but too much will fog the image with unimportant sub surface data, whilst too low a kV may not provide the resolution that you require. Microscopists are scientists we should experiment!
Good luck
Steve Chapman Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
----- Original Message ----- X-from: {mconstan-at-princeton.edu} To: {protrain-at-emcourses.com} Sent: Tuesday, January 30, 2007 3:10 AM
Hello colleagues,
I have (again) a pretty basic, non-life-threatening question about ultramicrotomy (with a Leica EM UC6 in my case). When I am cutting ultrathin sections it may happen I have to pause for a while (because I have to sign autographs, or to pick up sections from water). After the pause when I start the cutting cycle again, it very often does not cut immediately but I have to cut 500nm or more to touch the block again. I wondered if this was a sort of automatic protection from the machine, which retracts a little after some time of inactivity to avoid damage to the knife, or if this a the normal consequence of an effect of physics. Any comment on this?
Stephane
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==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Tue Jan 30 04:07:40 2007 6, 19 -- Received: from web37407.mail.mud.yahoo.com (web37407.mail.mud.yahoo.com [209.191.91.139]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0UA7eBg022570 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 04:07:40 -0600 6, 19 -- Received: (qmail 41697 invoked by uid 60001); 30 Jan 2007 10:07:40 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 6, 19 -- b=1+gBHYOW2Ee1SsEBAqxWuYWO5/nL8nn468i6i/JHanUAFCuYaxMvYlJD69RYqw8wN8Ck9AxdtzwfdWjcEPbq8VT8OKfknMmjNnA6uBWbdWqpeBvEE2CUqDpIOntBmBc9KIeJGijGR+OPFEeRXti883DSnoTmaxRoxUIQ3t718kI=; 6, 19 -- X-YMail-OSG: F4N2dwUVM1kyUlEszoDiJrQ93JszCzNI5jTxknPqdu3C.NSUiFGAGTXveQLO_7tHUG3Bz6CcIkJT4uNj48uba2WMjJiJYFTgohvdDVQ.1dKt8T8WSpk- 6, 19 -- Received: from [80.122.101.102] by web37407.mail.mud.yahoo.com via HTTP; Tue, 30 Jan 2007 02:07:40 PST 6, 19 -- Date: Tue, 30 Jan 2007 02:07:40 -0800 (PST) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 -- Subject: basic question ultramicrotopy 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- Message-ID: {389124.40167.qm-at-web37407.mail.mud.yahoo.com} ==============================End of - Headers==============================
Stephane, you are lucky - no accidental 1000nm sections! When I stop to sign autographs or explain to new users what is going on, the block in my Ultracut E sneaks forward and I demonstrate how to ruin the knife with a thick section. I accept this is probably due to operator error although an expert suggested that the block is compressed during sectioning and if left for a while will expand forward.
Dave
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: 30 January 2007 10:12 To: David Patton
Hello colleagues,
I have (again) a pretty basic, non-life-threatening question about ultramicrotomy (with a Leica EM UC6 in my case). When I am cutting ultrathin sections it may happen I have to pause for a while (because I have to sign autographs, or to pick up sections from water). After the pause when I start the cutting cycle again, it very often does not cut immediately but I have to cut 500nm or more to touch the block again. I wondered if this was a sort of automatic protection from the machine, which retracts a little after some time of inactivity to avoid damage to the knife, or if this a the normal consequence of an effect of physics. Any comment on this?
Stephane
________________________________________________________________________ ____________ It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. http://tools.search.yahoo.com/toolbar/features/mail/
==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Tue Jan 30 04:07:40 2007 6, 19 -- Received: from web37407.mail.mud.yahoo.com (web37407.mail.mud.yahoo.com [209.191.91.139]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0UA7eBg022570 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 04:07:40 -0600 6, 19 -- Received: (qmail 41697 invoked by uid 60001); 30 Jan 2007 10:07:40 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Co ntent-Transfer-Encoding:Message-ID; 6, 19 -- b=1+gBHYOW2Ee1SsEBAqxWuYWO5/nL8nn468i6i/JHanUAFCuYaxMvYlJD69RYqw8wN8Ck9A xdtzwfdWjcEPbq8VT8OKfknMmjNnA6uBWbdWqpeBvEE2CUqDpIOntBmBc9KIeJGijGR+OPFE eRXti883DSnoTmaxRoxUIQ3t718kI=; 6, 19 -- X-YMail-OSG: F4N2dwUVM1kyUlEszoDiJrQ93JszCzNI5jTxknPqdu3C.NSUiFGAGTXveQLO_7tHUG3Bz6Cc IkJT4uNj48uba2WMjJiJYFTgohvdDVQ.1dKt8T8WSpk- 6, 19 -- Received: from [80.122.101.102] by web37407.mail.mud.yahoo.com via HTTP; Tue, 30 Jan 2007 02:07:40 PST 6, 19 -- Date: Tue, 30 Jan 2007 02:07:40 -0800 (PST) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 -- Subject: basic question ultramicrotopy 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- Message-ID: {389124.40167.qm-at-web37407.mail.mud.yahoo.com} ==============================End of - Headers==============================
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==============================Original Headers============================== 18, 34 -- From David.Patton-at-uwe.ac.uk Tue Jan 30 05:57:16 2007 18, 34 -- Received: from mailapp03.uwe.ac.uk (mailapp03.uwe.ac.uk [164.11.132.65]) 18, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0UBvFst002960 18, 34 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 05:57:16 -0600 18, 34 -- Received: from (mta02.uwe.ac.uk [164.11.132.62]) by mailapp03.uwe.ac.uk with smtp 18, 34 -- id 6e3e_2833e114_b058_11db_8e32_00142221cca9; 18, 34 -- Tue, 30 Jan 2007 11:51:15 +0000 18, 34 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 18, 34 -- (egen-uwe01.uwe.ac.uk [164.11.249.121]) 18, 34 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 18, 34 -- 2005)) with ESMTP id {0JCO00GXCJV0WN-at-mta02.uwe.ac.uk} for 18, 34 -- microscopy-at-microscopy.com; Tue, 30 Jan 2007 11:57:01 +0000 (GMT) 18, 34 -- Date: Tue, 30 Jan 2007 11:51:13 +0000 18, 34 -- From: David Patton {David.Patton-at-uwe.ac.uk} 18, 34 -- Subject: RE: [Microscopy] basic question ultramicrotopy 18, 34 -- In-reply-to: {200701301011.l0UABfML029533-at-ns.microscopy.com} 18, 34 -- To: nizets2-at-yahoo.com 18, 34 -- Cc: microscopy-at-microscopy.com 18, 34 -- Message-id: {F247F674896BE243AD8263C5280E2BDB02B0B1E5-at-egen-uwe01} 18, 34 -- MIME-version: 1.0 18, 34 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 18, 34 -- Content-type: text/plain; charset=us-ascii 18, 34 -- Content-class: urn:content-classes:message 18, 34 -- Thread-topic: [Microscopy] basic question ultramicrotopy 18, 34 -- Thread-index: AcdEVxCgRwxzSHS8QpGuRKg0YxRSOwACYQBA 18, 34 -- X-MS-Has-Attach: 18, 34 -- X-MS-TNEF-Correlator: 18, 34 -- X-NAIMIME-Disclaimer: 1 18, 34 -- X-NAIMIME-Modified: 1 18, 34 -- X-NAI-Spam-Score: -2.5 18, 34 -- X-NAI-Spam-Rules: 1 Rules triggered 18, 34 -- BAYES_00=-2.5 18, 34 -- Content-Transfer-Encoding: 8bit 18, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0UBvFst002960 ==============================End of - Headers==============================
I have the opposite problem, maybe because I'm usually asking for autographs, instead of signing them. If I take a break, I learned the hard way that the first swipe of the block over the knife will usually cut off a section about as thick as a slice of bread. I've often wondered why----especially after one time losing a single layer of cells which floated off into the sunset. (I had spares, so no worries, mate.) We have Leica UCTs.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, January 30, 2007 4:08 AM To: Tindall, Randy D.
Hello colleagues,
I have (again) a pretty basic, non-life-threatening question about ultramicrotomy (with a Leica EM UC6 in my case). When I am cutting ultrathin sections it may happen I have to pause for a while (because I have to sign autographs, or to pick up sections from water). After the pause when I start the cutting cycle again, it very often does not cut immediately but I have to cut 500nm or more to touch the block again. I wondered if this was a sort of automatic protection from the machine, which retracts a little after some time of inactivity to avoid damage to the knife, or if this a the normal consequence of an effect of physics. Any comment on this?
Stephane
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==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Tue Jan 30 04:07:40 2007 6, 19 -- Received: from web37407.mail.mud.yahoo.com (web37407.mail.mud.yahoo.com [209.191.91.139]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0UA7eBg022570 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 04:07:40 -0600 6, 19 -- Received: (qmail 41697 invoked by uid 60001); 30 Jan 2007 10:07:40 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Co ntent-Transfer-Encoding:Message-ID; 6, 19 -- b=1+gBHYOW2Ee1SsEBAqxWuYWO5/nL8nn468i6i/JHanUAFCuYaxMvYlJD69RYqw8wN8Ck9A xdtzwfdWjcEPbq8VT8OKfknMmjNnA6uBWbdWqpeBvEE2CUqDpIOntBmBc9KIeJGijGR+OPFE eRXti883DSnoTmaxRoxUIQ3t718kI=; 6, 19 -- X-YMail-OSG: F4N2dwUVM1kyUlEszoDiJrQ93JszCzNI5jTxknPqdu3C.NSUiFGAGTXveQLO_7tHUG3Bz6Cc IkJT4uNj48uba2WMjJiJYFTgohvdDVQ.1dKt8T8WSpk- 6, 19 -- Received: from [80.122.101.102] by web37407.mail.mud.yahoo.com via HTTP; Tue, 30 Jan 2007 02:07:40 PST 6, 19 -- Date: Tue, 30 Jan 2007 02:07:40 -0800 (PST) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 -- Subject: basic question ultramicrotopy 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- Message-ID: {389124.40167.qm-at-web37407.mail.mud.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 17, 26 -- From TindallR-at-missouri.edu Tue Jan 30 08:19:51 2007 17, 26 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0UEJpVw016138 17, 26 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 08:19:51 -0600 17, 26 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 17, 26 -- Tue, 30 Jan 2007 08:19:50 -0600 17, 26 -- x-mimeole: Produced By Microsoft Exchange V6.5 17, 26 -- Content-class: urn:content-classes:message 17, 26 -- MIME-Version: 1.0 17, 26 -- Content-Type: text/plain; 17, 26 -- charset="us-ascii" 17, 26 -- Subject: RE: [Microscopy] basic question ultramicrotopy 17, 26 -- Date: Tue, 30 Jan 2007 08:19:51 -0600 17, 26 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68D85-at-UM-XMAIL08.um.umsystem.edu} 17, 26 -- In-Reply-To: {200701301008.l0UA8QDp024543-at-ns.microscopy.com} 17, 26 -- X-MS-Has-Attach: 17, 26 -- X-MS-TNEF-Correlator: 17, 26 -- Thread-Topic: [Microscopy] basic question ultramicrotopy 17, 26 -- Thread-Index: AcdEVpXqhzJvu355StiQwG7GHLeYTAAIprEg 17, 26 -- References: {200701301008.l0UA8QDp024543-at-ns.microscopy.com} 17, 26 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 17, 26 -- To: {nizets2-at-yahoo.com} 17, 26 -- Cc: {microscopy-at-microscopy.com} 17, 26 -- X-OriginalArrivalTime: 30 Jan 2007 14:19:50.0827 (UTC) FILETIME=[B43237B0:01C74479] 17, 26 -- Content-Transfer-Encoding: 8bit 17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0UEJpVw016138 ==============================End of - Headers==============================
I don't really think this is an operator error, it does happen all the time, standard or cryo, I am not sure why. After breaking a couple of cryoblocks (not ruining any diamond on plastic, so far), I always go 200-400 nm back (using coarse advance) when pausing for section retrieval. Usually I have to wait for 3-4 cycles for the microtome to start cutting again, but it solves the problem.
Michal
nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello colleagues, } } I have (again) a pretty basic, non-life-threatening } question about ultramicrotomy (with a Leica EM UC6 in } my case). } When I am cutting ultrathin sections it may happen I } have to pause for a while (because I have to sign } autographs, or to pick up sections from water). After } the pause when I start the cutting cycle again, it } very often does not cut immediately but I have to cut } 500nm or more to touch the block again. } I wondered if this was a sort of automatic protection } from the machine, which retracts a little after some } time of inactivity to avoid damage to the knife, or if } this a the normal consequence of an effect of physics. } Any comment on this? } } Stephane } } } } } ____________________________________________________________________________________ } It's here! Your new message! } Get new email alerts with the free Yahoo! Toolbar. } http://tools.search.yahoo.com/toolbar/features/mail/ } } ==============================Original Headers============================== } 6, 19 -- From nizets2-at-yahoo.com Tue Jan 30 04:07:40 2007 } 6, 19 -- Received: from web37407.mail.mud.yahoo.com (web37407.mail.mud.yahoo.com [209.191.91.139]) } 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l0UA7eBg022570 } 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 04:07:40 -0600 } 6, 19 -- Received: (qmail 41697 invoked by uid 60001); 30 Jan 2007 10:07:40 -0000 } 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 6, 19 -- s=s1024; d=yahoo.com; } 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; } 6, 19 -- b=1+gBHYOW2Ee1SsEBAqxWuYWO5/nL8nn468i6i/JHanUAFCuYaxMvYlJD69RYqw8wN8Ck9AxdtzwfdWjcEPbq8VT8OKfknMmjNnA6uBWbdWqpeBvEE2CUqDpIOntBmBc9KIeJGijGR+OPFEeRXti883DSnoTmaxRoxUIQ3t718kI=; } 6, 19 -- X-YMail-OSG: F4N2dwUVM1kyUlEszoDiJrQ93JszCzNI5jTxknPqdu3C.NSUiFGAGTXveQLO_7tHUG3Bz6CcIkJT4uNj48uba2WMjJiJYFTgohvdDVQ.1dKt8T8WSpk- } 6, 19 -- Received: from [80.122.101.102] by web37407.mail.mud.yahoo.com via HTTP; Tue, 30 Jan 2007 02:07:40 PST } 6, 19 -- Date: Tue, 30 Jan 2007 02:07:40 -0800 (PST) } 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 6, 19 -- Subject: basic question ultramicrotopy } 6, 19 -- To: microscopy-at-microscopy.com } 6, 19 -- MIME-Version: 1.0 } 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 } 6, 19 -- Content-Transfer-Encoding: 8bit } 6, 19 -- Message-ID: {389124.40167.qm-at-web37407.mail.mud.yahoo.com} } ==============================End of - Headers============================== }
==============================Original Headers============================== 3, 19 -- From Michal.Jarnik-at-fccc.edu Tue Jan 30 08:40:45 2007 3, 19 -- Received: from azah.fccc.edu (azah.fccc.edu [131.249.4.237]) 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0UEejgq027634 3, 19 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 08:40:45 -0600 3, 19 -- Received: from [131.249.6.85] (emf1.fccc.edu [131.249.6.85]) 3, 19 -- (authenticated bits=0) 3, 19 -- by azah.fccc.edu (8.13.6/8.13.6) with ESMTP id l0UEejK7011668 3, 19 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 09:40:45 -0500 (EST) 3, 19 -- Message-ID: {45BF58E9.4050809-at-fccc.edu} 3, 19 -- Date: Tue, 30 Jan 2007 09:40:41 -0500 3, 19 -- From: Michal Jarnik {Michal.Jarnik-at-fccc.edu} 3, 19 -- User-Agent: Thunderbird 1.5.0.5 (Macintosh/20060719) 3, 19 -- MIME-Version: 1.0 3, 19 -- To: microscopy-at-microscopy.com 3, 19 -- Subject: Re: [Microscopy] basic question ultramicrotopy 3, 19 -- References: {200701301013.l0UADfhx032601-at-ns.microscopy.com} 3, 19 -- In-Reply-To: {200701301013.l0UADfhx032601-at-ns.microscopy.com} 3, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I must be doing something very wrong - I'm only called away from the microtome to be informed of malfunctioning microscopes/gel boxes/processors/etc. - never to sign autographs. When I return from the crisis, sometimes the block is further forward, sometimes back - I always retract the knife out of paranoia. I think the block moving away from the knife is due to thermal effects; you may generate enough friction during cutting to cause thermal expansion of the block, which then shrinks back when you stop cutting. At least, that is what I was told when I was learning to cut. I think it moves forward out of pure contrariness. I know on the old thermal advance microtomes you were pretty much guaranteed a trashed block/knife if you didn't retract when re-starting...there were all kinds of odd behaviors associated with those machines.
Tamara
On Tue, 30 Jan 2007 04:08:34 -0600 nizets2-at-yahoo.com wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello colleagues, } } I have (again) a pretty basic, non-life-threatening } question about ultramicrotomy (with a Leica EM UC6 in } my case). } When I am cutting ultrathin sections it may happen I } have to pause for a while (because I have to sign } autographs, or to pick up sections from water). After } the pause when I start the cutting cycle again, it } very often does not cut immediately but I have to cut } 500nm or more to touch the block again. } I wondered if this was a sort of automatic protection } from the machine, which retracts a little after some } time of inactivity to avoid damage to the knife, or if } this a the normal consequence of an effect of physics. } Any comment on this? } } Stephane } } } } } ____________________________________________________________________________________ } It's here! Your new message! } Get new email alerts with the free Yahoo! Toolbar. } http://tools.search.yahoo.com/toolbar/features/mail/ } } ==============================Original } Headers============================== } 6, 19 -- From nizets2-at-yahoo.com Tue Jan 30 04:07:40 2007 } 6, 19 -- Received: from web37407.mail.mud.yahoo.com } (web37407.mail.mud.yahoo.com [209.191.91.139]) } 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
On a related theme. Without pausing to sign autographs etc has anyone demonstrated that turning the "wheel" only moves the block up and down, only to have puzzled students say "But it is cutting sections."?
Dave
-----Original Message----- X-from: thoward-at-unm.edu [mailto:thoward-at-unm.edu] Sent: 30 January 2007 15:06 To: David Patton
I must be doing something very wrong - I'm only called away from the microtome to be informed of malfunctioning microscopes/gel boxes/processors/etc. - never to sign autographs. When I return from the crisis, sometimes the block is further forward, sometimes back - I always retract the knife out of paranoia. I think the block moving away from the knife is due to thermal effects; you may generate enough friction during cutting to cause thermal expansion of the block, which then shrinks back when you stop cutting. At least, that is what I was told when I was learning to cut. I think it moves forward out of pure contrariness. I know on the old thermal advance microtomes you were pretty much guaranteed a trashed block/knife if you didn't retract when re-starting...there were all kinds of odd behaviors associated with those machines.
Tamara
On Tue, 30 Jan 2007 04:08:34 -0600 nizets2-at-yahoo.com wrote: } } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Hello colleagues, } } I have (again) a pretty basic, non-life-threatening } question about ultramicrotomy (with a Leica EM UC6 in } my case). } When I am cutting ultrathin sections it may happen I } have to pause for a while (because I have to sign } autographs, or to pick up sections from water). After } the pause when I start the cutting cycle again, it } very often does not cut immediately but I have to cut } 500nm or more to touch the block again. } I wondered if this was a sort of automatic protection } from the machine, which retracts a little after some } time of inactivity to avoid damage to the knife, or if } this a the normal consequence of an effect of physics. } Any comment on this? } } Stephane } } } } } ________________________________________________________________________ ____________ } It's here! Your new message! } Get new email alerts with the free Yahoo! Toolbar. } http://tools.search.yahoo.com/toolbar/features/mail/ } } ==============================Original } Headers============================== } 6, 19 -- From nizets2-at-yahoo.com Tue Jan 30 04:07:40 2007 } 6, 19 -- Received: from web37407.mail.mud.yahoo.com } (web37407.mail.mud.yahoo.com [209.191.91.139]) } 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
The chucked block in the unattended ultramicrotome obeys the Hehesenberg Uncertainty Principle. As I trust you remember the position of a chucked block whose momentary momentum is zero will come to rest at a less accurate location. When the circular motion of the microtome wheel approaches zero then the product of the position and momentum approaches the Kerplank's constant. Thus using more mathematical rigor the conjugate quantity of the sections becomes the standard deviation from the property of being silver. Associated with this pause in the angular momentum of the microtome will be section chatter due to wave-particle duality. Ultramicrotome manufactures have tried to address this problem with an adaptor known as the von Neumann measurement, which according to some is better than the Landau calculation.
I hope recalling this information lays to rest in no uncertain terms the phenomenon of zero momentum block creep or in some cases inverse block creep.
Bob Blystone
==============================Original Headers============================== 6, 17 -- From rblyston-at-trinity.edu Tue Jan 30 09:31:11 2007 6, 17 -- Received: from ms-smtp-04.texas.rr.com (ms-smtp-04.texas.rr.com [24.93.47.43]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0UFVBCS029656 6, 17 -- for {Microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 09:31:11 -0600 6, 17 -- Received: from [192.168.0.2] (cpe-66-69-93-33.satx.res.rr.com [66.69.93.33]) 6, 17 -- by ms-smtp-04.texas.rr.com (8.13.6/8.13.6) with ESMTP id l0UFV7IW003436 6, 17 -- for {Microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 09:31:08 -0600 (CST) 6, 17 -- Mime-Version: 1.0 (Apple Message framework v752.2) 6, 17 -- Content-Transfer-Encoding: 7bit 6, 17 -- Message-Id: {32791431-6E70-43AE-BC6B-BCEF6BEFCD19-at-trinity.edu} 6, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 17 -- To: Microscopy-at-microscopy.com 6, 17 -- From: Robert Blystone {rblyston-at-trinity.edu} 6, 17 -- Subject: Differential Ultramicrotomy 6, 17 -- Date: Tue, 30 Jan 2007 09:31:04 -0600 6, 17 -- X-Mailer: Apple Mail (2.752.2) 6, 17 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
-----Original Message----- X-from: rblyston-at-trinity.edu [mailto:rblyston-at-trinity.edu] Sent: Tuesday, January 30, 2007 9:32 AM To: Tindall, Randy D.
To all:
The chucked block in the unattended ultramicrotome obeys the Hehesenberg Uncertainty Principle. As I trust you remember the position of a chucked block whose momentary momentum is zero will come to rest at a less accurate location. When the circular motion of the microtome wheel approaches zero then the product of the position and momentum approaches the Kerplank's constant. Thus using more mathematical rigor the conjugate quantity of the sections becomes the standard deviation from the property of being silver. Associated with this pause in the angular momentum of the microtome will be section chatter due to wave-particle duality. Ultramicrotome manufactures have tried to address this problem with an adaptor known as the von Neumann measurement, which according to some is better than the Landau calculation.
I hope recalling this information lays to rest in no uncertain terms the phenomenon of zero momentum block creep or in some cases inverse block creep.
Bob Blystone
==============================Original Headers============================== 6, 17 -- From rblyston-at-trinity.edu Tue Jan 30 09:31:11 2007 6, 17 -- Received: from ms-smtp-04.texas.rr.com (ms-smtp-04.texas.rr.com [24.93.47.43]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0UFVBCS029656 6, 17 -- for {Microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 09:31:11 -0600 6, 17 -- Received: from [192.168.0.2] (cpe-66-69-93-33.satx.res.rr.com [66.69.93.33]) 6, 17 -- by ms-smtp-04.texas.rr.com (8.13.6/8.13.6) with ESMTP id l0UFV7IW003436 6, 17 -- for {Microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 09:31:08 -0600 (CST) 6, 17 -- Mime-Version: 1.0 (Apple Message framework v752.2) 6, 17 -- Content-Transfer-Encoding: 7bit 6, 17 -- Message-Id: {32791431-6E70-43AE-BC6B-BCEF6BEFCD19-at-trinity.edu} 6, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 17 -- To: Microscopy-at-microscopy.com 6, 17 -- From: Robert Blystone {rblyston-at-trinity.edu} 6, 17 -- Subject: Differential Ultramicrotomy 6, 17 -- Date: Tue, 30 Jan 2007 09:31:04 -0600 6, 17 -- X-Mailer: Apple Mail (2.752.2) 6, 17 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
==============================Original Headers============================== 15, 26 -- From TindallR-at-missouri.edu Tue Jan 30 09:36:46 2007 15, 26 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 15, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0UFakrK007626 15, 26 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 09:36:46 -0600 15, 26 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 15, 26 -- Tue, 30 Jan 2007 09:36:46 -0600 15, 26 -- x-mimeole: Produced By Microsoft Exchange V6.5 15, 26 -- Content-class: urn:content-classes:message 15, 26 -- MIME-Version: 1.0 15, 26 -- Content-Type: text/plain; 15, 26 -- charset="us-ascii" 15, 26 -- Subject: RE: [Microscopy] Differential Ultramicrotomy 15, 26 -- Date: Tue, 30 Jan 2007 09:36:46 -0600 15, 26 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68D88-at-UM-XMAIL08.um.umsystem.edu} 15, 26 -- In-Reply-To: {200701301532.l0UFWJKo031669-at-ns.microscopy.com} 15, 26 -- X-MS-Has-Attach: 15, 26 -- X-MS-TNEF-Correlator: 15, 26 -- Thread-Topic: [Microscopy] Differential Ultramicrotomy 15, 26 -- Thread-Index: AcdEg9Tipa8emWkETBKffY/6uJLcTQAAF7eA 15, 26 -- References: {200701301532.l0UFWJKo031669-at-ns.microscopy.com} 15, 26 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 15, 26 -- To: {rblyston-at-trinity.edu} 15, 26 -- Cc: {microscopy-at-microscopy.com} 15, 26 -- X-OriginalArrivalTime: 30 Jan 2007 15:36:46.0011 (UTC) FILETIME=[730F68B0:01C74484] 15, 26 -- Content-Transfer-Encoding: 8bit 15, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0UFakrK007626 ==============================End of - Headers==============================
And I thought it was loads more complicated than that!
Pet
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-- Peter Bond Scientific Officer Plymouth Electron Microscopy Centre University of Plymouth Drake Circus Plymouth Devon UK PL4 8AA Tel: 44 (0)1752 233092 pbond-at-plymouth.ac.uk http://www.plymouth.ac.uk/emc
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During the cutting process, the blade is compressing the remainder of the block, which then expands afterwards. My mentors led me to believe the overlap with the knife was due to expansion of the block whenever it was allowed to 'rest'. The forces equalize when sectioning steadily. This is one reason microtomes for ulta-thin or semi-thin ( {3 µm) sections retract during the return stroke. I don't know if this is really the case, but it does seem to account for the fact that we can break knives and blocks if not separated before resuming sectioning.
Regards, Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
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On Jan 30, 2007, at 2:09 AM, nizets2-at-yahoo.com wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hello colleagues, } } I have (again) a pretty basic, non-life-threatening } question about ultramicrotomy (with a Leica EM UC6 in } my case). } When I am cutting ultrathin sections it may happen I } have to pause for a while (because I have to sign } autographs, or to pick up sections from water). After } the pause when I start the cutting cycle again, it } very often does not cut immediately but I have to cut } 500nm or more to touch the block again. } I wondered if this was a sort of automatic protection } from the machine, which retracts a little after some } time of inactivity to avoid damage to the knife, or if } this a the normal consequence of an effect of physics. } Any comment on this? } } Stephane } } } } } ______________________________________________________________________ } ______________ } It's here! Your new message! } Get new email alerts with the free Yahoo! Toolbar. } http://tools.search.yahoo.com/toolbar/features/mail/ } } ==============================Original } Headers============================== } 6, 19 -- From nizets2-at-yahoo.com Tue Jan 30 04:07:40 2007 } 6, 19 -- Received: from web37407.mail.mud.yahoo.com } (web37407.mail.mud.yahoo.com [209.191.91.139]) } 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id l0UA7eBg022570 } 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 } 04:07:40 -0600 } 6, 19 -- Received: (qmail 41697 invoked by uid 60001); 30 Jan 2007 } 10:07:40 -0000 } 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 6, 19 -- s=s1024; d=yahoo.com; } 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME- } Version:Content-Type:Content-Transfer-Encoding:Message-ID; } 6, 19 -- b=1+gBHYOW2Ee1SsEBAqxWuYWO5/nL8nn468i6i/ } JHanUAFCuYaxMvYlJD69RYqw8wN8Ck9AxdtzwfdWjcEPbq8VT8OKfknMmjNnA6uBWbdWqp } eBvEE2CUqDpIOntBmBc9KIeJGijGR+OPFEeRXti883DSnoTmaxRoxUIQ3t718kI=; } 6, 19 -- X-YMail-OSG: } F4N2dwUVM1kyUlEszoDiJrQ93JszCzNI5jTxknPqdu3C.NSUiFGAGTXveQLO_7tHUG3Bz6 } CcIkJT4uNj48uba2WMjJiJYFTgohvdDVQ.1dKt8T8WSpk- } 6, 19 -- Received: from [80.122.101.102] by } web37407.mail.mud.yahoo.com via HTTP; Tue, 30 Jan 2007 02:07:40 PST } 6, 19 -- Date: Tue, 30 Jan 2007 02:07:40 -0800 (PST) } 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 6, 19 -- Subject: basic question ultramicrotopy } 6, 19 -- To: microscopy-at-microscopy.com } 6, 19 -- MIME-Version: 1.0 } 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 } 6, 19 -- Content-Transfer-Encoding: 8bit } 6, 19 -- Message-ID: {389124.40167.qm-at-web37407.mail.mud.yahoo.com} } ==============================End of - } Headers==============================
==============================Original Headers============================== 9, 27 -- From glenmac-at-u.washington.edu Tue Jan 30 11:47:30 2007 9, 27 -- Received: from mxout2.cac.washington.edu (mxout2.cac.washington.edu [140.142.33.4]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0UHlUkJ001376 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 11:47:30 -0600 9, 27 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.139]) 9, 27 -- by mxout2.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW06.09) with ESMTP id l0UHlTxE009278 9, 27 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 09:47:29 -0800 9, 27 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) 9, 27 -- (authenticated authid=glenmac) 9, 27 -- by smtp.washington.edu (8.13.7+UW06.06/8.13.7+UW06.09) with ESMTP id l0UHlTqn003865 9, 27 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 09:47:29 -0800 9, 27 -- Mime-Version: 1.0 (Apple Message framework v752.2) 9, 27 -- In-Reply-To: {200701301009.l0UA9Yqu025080-at-ns.microscopy.com} 9, 27 -- References: {200701301009.l0UA9Yqu025080-at-ns.microscopy.com} 9, 27 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 9, 27 -- Message-Id: {F61A3F01-BA57-4E26-8E7C-5FF4E04D8C08-at-u.washington.edu} 9, 27 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 9, 27 -- Subject: Re: [Microscopy] basic question ultramicrotopy 9, 27 -- Date: Tue, 30 Jan 2007 09:47:24 -0800 9, 27 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} 9, 27 -- X-Mailer: Apple Mail (2.752.2) 9, 27 -- X-PMX-Version: 5.3.0.289146, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.1.30.93432 9, 27 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0' 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0UHlUkJ001376 ==============================End of - Headers==============================
Our digital TEM camera is dead, the TEM is on it's last leg so I am not going to have the camera repaired. We use this TEM for teaching and it is difficult to teach without a camera. I am thinking that there may be a way to install a removable low cost video camera on the binocular eyepiece for some applications - like teaching. Has anyone done this?
Owen
Owen P. Mills Director, Materials Characterization & Fabrication Facilities Electron Optics Engineer, Applied Chemical & Morphological Analysis Laboratory
Materials Science & Engineering Michigan Technological University Rm 512 M&M Bldg. Houghton, MI 49931 PH 906-369-1875 FAX 906-487-2934 mailto:opmills-at-mtu.edu http://www.mm.mtu.edu/~opmills
==============================Original Headers============================== 7, 31 -- From opmills-at-mtu.edu Tue Jan 30 12:05:14 2007 7, 31 -- Received: from node8.mtu.edu (node8.mtu.edu [141.219.69.8]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0UI5EhD012715 7, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 12:05:14 -0600 7, 31 -- Received: from node5.edge.dcsint.mtu.edu (node5.mtu.edu [141.219.69.5]) 7, 31 -- by node8.mtu.edu (8.13.1/8.13.1) with ESMTP id l0UI5Cbh028990 7, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 13:05:12 -0500 7, 31 -- Received: from node34.edge.dcsint.mtu.edu (node34.mtu.edu [141.219.69.34]) 7, 31 -- by node5.edge.dcsint.mtu.edu (8.12.11.20060308/8.12.8) with ESMTP id l0UI5CDK016955 7, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 13:05:12 -0500 7, 31 -- Received: from mail.mtu.edu (campus0.mtu.edu [141.219.70.8]) 7, 31 -- by node34.edge.dcsint.mtu.edu (8.13.8/8.13.8) with ESMTP id l0UI5B9s005195 7, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 13:05:11 -0500 7, 31 -- (envelope-from opmills-at-mtu.edu) 7, 31 -- Received: from node6.edge.dcsint.mtu.edu (node6.mtu.edu [141.219.69.6]) 7, 31 -- by mail.mtu.edu (8.13.6+Sun/8.11.6) with ESMTP id l0UI5BHC004120 7, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 13:05:11 -0500 (EST) 7, 31 -- Received: from [141.219.192.45] (opmills.eecn.sabo.mtu.edu [141.219.192.45]) 7, 31 -- by node6.edge.dcsint.mtu.edu (8.12.11.20060308/8.12.3/auth_ssl.mc v1.0) with ESMTP id l0UI5BwO030489 7, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 13:05:11 -0500 7, 31 -- Mime-Version: 1.0 (Apple Message framework v752.3) 7, 31 -- Content-Transfer-Encoding: 7bit 7, 31 -- Message-Id: {248461F6-4D9A-4071-8C6C-8B30D858EE0E-at-mtu.edu} 7, 31 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 31 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 31 -- From: "Owen P. Mills" {opmills-at-mtu.edu} 7, 31 -- Subject: poor mans TEM camera 7, 31 -- Date: Tue, 30 Jan 2007 13:05:08 -0500 7, 31 -- X-Mailer: Apple Mail (2.752.3) 7, 31 -- X-PMX-Version: 5.3.0.289146, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.1.30.95432 7, 31 -- X-PerlMx-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
Does anybody happen to know of a reference or resource for the size and tolerance specifications for a conventional 25x75 mm microscope slide? Thanks in advance.
-- Karl Garsha Head Applications Scientist Roper Bioscience Advanced Microimaging Group 3440 E. Brittania Drive Tucson, AZ 85706 Office: 520-547-2704
==============================Original Headers============================== 3, 27 -- From garsha-at-itg.uiuc.edu Tue Jan 30 14:55:31 2007 3, 27 -- Received: from outmail128166.authsmtp.net (outmail128166.authsmtp.net [62.13.128.166]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0UKtUAi028471 3, 27 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 14:55:31 -0600 3, 27 -- Received: from outmail128185.authsmtp.co.uk (outmail128185.authsmtp.co.uk [62.13.128.185]) 3, 27 -- by punt2.authsmtp.com (8.13.8/8.13.8/Kp) with ESMTP id l0UKtSv8057612 3, 27 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 20:55:29 GMT 3, 27 -- Received: from [144.22.2.112] (66-162-43-45.static.twtelecom.net [66.162.43.45]) 3, 27 -- (authenticated bits=0) 3, 27 -- by mail.authsmtp.com (8.13.8/8.13.8/Kp) with ESMTP id l0UKtM3P044076 3, 27 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 20:55:23 GMT 3, 27 -- Message-ID: {45BFB0B6.709-at-itg.uiuc.edu} 3, 27 -- Date: Tue, 30 Jan 2007 13:55:18 -0700 3, 27 -- From: Karl Garsha {garsha-at-itg.uiuc.edu} 3, 27 -- Reply-To: kgarsha-at-roperscientific.com 3, 27 -- Organization: Roper Scientific 3, 27 -- User-Agent: Thunderbird 1.5.0.9 (Windows/20061207) 3, 27 -- MIME-Version: 1.0 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: tolerance specifications for standard microscope slide? 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- X-Server-Quench: 34aa796d-b0a4-11db-8638-001185d377ca 3, 27 -- X-AuthRoute: OCdyaAgTClZaRR4B CiosDTNPDxgkOxYK DBMeOw5bK0AOTg9W KldyK1tYKloHTlZB SnhYBAkaU11uIzIr dAhRbQJNYEpEWgBg UkwHRFRMFQNqHxgC GBobTRt3dQBZeDAs ZSMZGg1YPER+cUZ6 RABTE25IZmMxOWcW VhRFJQMFdB5Lf0sX d1h5V3sQYWUGZ3Jl E1RsYDs4KylYLClP SwcGIBoJWUsAHzA9 TBkeHDIpBgULQD97 NAAvLFIVBkpZN0Q5 K1w6UlUAPlcJDgxS EhYl 3, 27 -- X-Authentic-SMTP: 61633138303639.squirrel.dmpriest.net.uk:536/Kp 3, 27 -- X-Report-SPAM: If SPAM / abuse - report it at: http://www.authsmtp.com/abuse 3, 27 -- X-Virus-Status: No virus detected - but ensure you scan with your own anti-virus system! ==============================End of - Headers==============================
I've had it both ways; block retracts and when I restart it misses, or block expands and when I restart it whacks the knife. Depends on the type of resin, how hard it is, the sample in the resin, temperature, humidity, all kinds of things. Some of my stuff compresses with lengthy sectioning and the "relaxes" forward when I stop. Other times the block seems to heat up and be expanding during sectioning, then cools off and retracts when I stop. In all cases I have learned to retract the knife before starting again, just in case. Annoying, yes, but better safe than sorry.
I had one customer who had lots of material embedded in LR White many, many years ago, and brought them in whenever she wanted to try a new antibody. I had become familiar with them and knew that over time the tissue (squid parts) expanded out of the face of the blocks. I guess the tissue was softer than the surrounding resin. I used this "feature" once when all the TEMs in the state were down and they were desparate to see if they had immunogold staining. I put the labeled grids in the SEM and was able to see the features of the tissue by topography, and the colloidal gold sitting on top.
Please; no autographs!
Aloha, Tina
} I have (again) a pretty basic, non-life-threatening question about } ultramicrotomy (with a Leica EM UC6 in my case). } When I am cutting ultrathin sections it may happen I have to pause for a } while (because I have to sign autographs, or to pick up sections from } water). After the pause when I start the cutting cycle again, it very } often does not cut immediately but I have to cut 500nm or more to touch } the block again. } I wondered if this was a sort of automatic protection from the machine, } which retracts a little after some time of inactivity to avoid damage to } the knife, or if this a the normal consequence of an effect of physics. } Any comment on this? } } Stephane } } } } } ________________________________________________________________________ } ____________ } It's here! Your new message! } Get new email alerts with the free Yahoo! Toolbar. } http://tools.search.yahoo.com/toolbar/features/mail/ } } ==============================Original } Headers============================== } 6, 19 -- From nizets2-at-yahoo.com Tue Jan 30 04:07:40 2007 6, 19 -- } Received: from web37407.mail.mud.yahoo.com (web37407.mail.mud.yahoo.com } [209.191.91.139]) } 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id l0UA7eBg022570 } 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 } 04:07:40 -0600 } 6, 19 -- Received: (qmail 41697 invoked by uid 60001); 30 Jan 2007 } 10:07:40 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 6, 19 -- s=s1024; d=yahoo.com; } 6, 19 -- } h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Co } ntent-Transfer-Encoding:Message-ID; } 6, 19 -- } b=1+gBHYOW2Ee1SsEBAqxWuYWO5/nL8nn468i6i/JHanUAFCuYaxMvYlJD69RYqw8wN8Ck9A } xdtzwfdWjcEPbq8VT8OKfknMmjNnA6uBWbdWqpeBvEE2CUqDpIOntBmBc9KIeJGijGR+OPFE } eRXti883DSnoTmaxRoxUIQ3t718kI=; } 6, 19 -- X-YMail-OSG: } F4N2dwUVM1kyUlEszoDiJrQ93JszCzNI5jTxknPqdu3C.NSUiFGAGTXveQLO_7tHUG3Bz6Cc } IkJT4uNj48uba2WMjJiJYFTgohvdDVQ.1dKt8T8WSpk- } 6, 19 -- Received: from [80.122.101.102] by web37407.mail.mud.yahoo.com } via HTTP; Tue, 30 Jan 2007 02:07:40 PST 6, 19 -- Date: Tue, 30 Jan 2007 } 02:07:40 -0800 (PST) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 6, 19 -- Subject: basic question ultramicrotopy 6, 19 -- To: } microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- } Content-Type: text/plain; charset=iso-8859-1 6, 19 -- } Content-Transfer-Encoding: 8bit 6, 19 -- Message-ID: } {389124.40167.qm-at-web37407.mail.mud.yahoo.com} } ==============================End of - } Headers============================== } } } } ==============================Original Headers============================== } 17, 26 -- From TindallR-at-missouri.edu Tue Jan 30 08:19:51 2007 } 17, 26 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) } 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0UEJpVw016138 } 17, 26 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jan 2007 08:19:51 -0600 } 17, 26 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); } 17, 26 -- Tue, 30 Jan 2007 08:19:50 -0600 } 17, 26 -- x-mimeole: Produced By Microsoft Exchange V6.5 } 17, 26 -- Content-class: urn:content-classes:message } 17, 26 -- MIME-Version: 1.0 } 17, 26 -- Content-Type: text/plain; } 17, 26 -- charset="us-ascii" } 17, 26 -- Subject: RE: [Microscopy] basic question ultramicrotopy } 17, 26 -- Date: Tue, 30 Jan 2007 08:19:51 -0600 } 17, 26 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68D85-at-UM-XMAIL08.um.umsystem.edu} } 17, 26 -- In-Reply-To: {200701301008.l0UA8QDp024543-at-ns.microscopy.com} } 17, 26 -- X-MS-Has-Attach: } 17, 26 -- X-MS-TNEF-Correlator: } 17, 26 -- Thread-Topic: [Microscopy] basic question ultramicrotopy } 17, 26 -- Thread-Index: AcdEVpXqhzJvu355StiQwG7GHLeYTAAIprEg } 17, 26 -- References: {200701301008.l0UA8QDp024543-at-ns.microscopy.com} } 17, 26 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 17, 26 -- To: {nizets2-at-yahoo.com} } 17, 26 -- Cc: {microscopy-at-microscopy.com} } 17, 26 -- X-OriginalArrivalTime: 30 Jan 2007 14:19:50.0827 (UTC) FILETIME=[B43237B0:01C74479] } 17, 26 -- Content-Transfer-Encoding: 8bit } 17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l0UEJpVw016138 } ==============================End of - Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 10, 19 -- From tina-at-pbrc.hawaii.edu Tue Jan 30 14:59:18 2007 10, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0UKxHKw032484 10, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 30 Jan 2007 14:59:18 -0600 10, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 10, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id l0UKxDEw029309 10, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 10, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 30 Jan 2007 10:59:14 -1000 (HST) 10, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id l0UKxAOc029306 10, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 30 Jan 2007 10:59:13 -1000 (HST) 10, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 10, 19 -- Date: Tue, 30 Jan 2007 10:59:10 -1000 (HST) 10, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 10, 19 -- X-Sender: tina-at-halia 10, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 10, 19 -- Subject: Re: [Microscopy] RE: basic question ultramicrotopy (fwd) 10, 19 -- Message-ID: {Pine.GSO.4.21.0701301045190.28756-100000-at-halia} 10, 19 -- MIME-Version: 1.0 10, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
Your question has three answers. The block can move away from the knife edge, towards it, or stay at the same distance. The main governing factors are the mechanical advance setting, temperature, and other heating (or cooling) affects.
Most microtomists would like to believe that a mechanical advance setting on a microtome determines the final thickness of sections. The mechanical advance is a guideline or course adjustment of how thick your sections are. The final thickness is the sum of the positive mechanical advance and the ± thermal advance. The section's interference color tells you how thick the section really is as it floats on water. This assumes you are not cutting something like polyurethanes or high index eyewear lenses which "mess up" or shift the interference colors on a thickness chart.
Your gap is caused by a discontinuous use of the mechanical advance and block cutting while thermal affects continue to operate and you sign your notebook. If the block, arm or stage are warm and cooling, the gap will be seen as you described. If the block is cold and warming up, then the thin sections will be too thick. If all the temperatures of all the equipment, the air, and your body & fingers are the same; then the mechanical advance setting will probably be real.
It was not unusual for me to see the block retreat from the knife edge at first, later stabilize, and finally advance towards the knife edge during a thin sectioning session on one block. You have to remember that your body is giving off heat, you are touching adjustments on the chuck or arc segment holder, the air temp might be varying, you picked up the diamond knife and mounted it, you put cooler water in the knife boat, the internal electronics are heating the cutting arm, water from the boat cools the knife edge and block face, or maybe you just took off a cryo unit and the arm and stage are still warming up. These thermal conditions have affects on thickness' (and your gap).
If you are going to stop cutting for a few minutes, you should always back up the knife about 5-10 microns and place the arm in the recommended position. The knife should never be parked in front of the block face. Failure to do so can result in a block of resin being shattered and/or a knife being ruined.
One time I was cutting a very long and 0.25 mm wide block of an automotive coating in cross section. My lab was located on bedrock and the microtome was on an isolation table. My sections were being cut and I was getting bright gold sections. I started the next section and it was gold. A guy walked up to me and stood next to the microtome table but off to the side. He was three feet away. The middle of that section changed to another interference color. Just his standing there made a difference in the interference color on the rest of that cut. I usually told these people to not move and finished the next two sections and stopped.
Once I had stopped cutting a block and backed up the knife. When I returned, the block face did not face off uniformly. It would cut a wedge shape from right to left. That meant the block was deforming under just the pressure of the clamping and I was using a "hard" Epon 812 clone epoxy formulation.
The number of variables in use during microtoming of blocks for TEM quality thin sections is high and sometimes subtle.
AO Reichert sold or gave away at their convention booths, a nice booklet on microtoming. I think it was called "Ultramicrotomy---Faults and Problems". It was a blue color and old. Maybe someone on the list can tell you how to get a copy. It's worth getting but it is not a textbook. It was more like a reprint of an article.
Microtomy is a learned skill just like riding a bicycle, only harder. Some people just can't do it very well. Others could and we said they had "the touch".
Paul
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Try http://www.supercircuits.com/ and type PC164C in search box. Sensitivity will be adequate. Resolution is NTSC with composite video out. Can be viewed on TV security monitor and/or digitized with inexpensive USB digitizer from a computer store. It will work on a binocular with eyepiece removed. You may have to use an adapter for an eyepiece (as for microscope/telescope) if you care about particular field of view.
I would use instead a suitable C-mount lens and a gooseneck support from a desk lamp - camera weight including lens is only a couple of ounces. Position camera next to binoculars, and point it at a (tilted forward) large viewing screen.
Should fit within $350 budget (camera, lens, power supply, USB digitizer or a TV security monitor). Will work with some limitations...
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com ----- Original Message ----- X-from: {opmills-at-mtu.edu} To: {vitalylazar-at-att.net} Sent: Tuesday, January 30, 2007 1:06 PM
Karl Garsha wrote: ============================================== Does anybody happen to know of a reference or resource for the size and tolerance specifications for a conventional 25x75 mm microscope slide? Thanks in advance. =============================================== What is "conventional" in one part of the world is not necessarily the same as what would be "conventional" in some other part of the world. So-called standard microscope slides are made both as 1" x 3" and in other instances, 25 x 75 mm. It usually does not make a difference but if in your case it does, it is less a matter of talking about tolerances than it is in terms of specifying the right size. Probably the 1" x 3" are more commonly available.
There are other instances in the microscopy world where we have a similar kind of "situation". For example, the small "JEOL" SEM mounts, in North America are specified as 3/8" but in other countries, they are specified as 10 mm. In general, the mounts are interchangeable in the various SEMs but the problem comes when one wants to put these mounts into storage boxes. Those made for 3/8" won't accommodate a 10 mm mount, and vice versa.
Chuck
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Title-Subject: [Filtered] Job outlook for microscopy technicians
Question: Hello. I am considering undertaking a two-year associate degree program to become a microscopy technician. I was wondering if anyone would be willing to comment on the job prospects I might have as new graduate with no science experience beyond this associate degree. The college's placement stats. look good but I would like a second opinion. Also, I am in my early forties and wonder if that would work against me finding a job (one of the draws of microscopy is that it is such a specialized skill that I would think employers would look beyond age). Thanks for any help you can give me.
Dear Bea, I can address your concern from my perspective as the director of a Core microscopy facility at an Ivy League Med school. When I am in the market for new staff, it is like a breath of fresh air to find someone with training. In the past 6 years, I've hired 2 techs, both of whom were fresh out of college (4-year). Each of them had exposure to, but no experience in microscopy and needed training from the ground up. I was fortunate that tech #2 started working while tech #1 was still here, so that #1 could train #2, with less imput from me. The first time around, I had to do all of the hands-on training, and it took me away from much of my other work. Finding someone with a microscopy background would be a godsend. Good luck, Lee Cohen-Gould -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Weill-Cornell Medical College
I agree with Leona that the job possibilities in microscopy are good for people with experience and training, and I suspect that being in your 40's would not be a significant factor. It may even work in your favor, since people (at least the ones who have never met me) might assume that age equals maturity, stability, responsibility, etc.
That said, there is a factor that is seldom discussed, but definitely exists. While virtually all job advertisements ask for experience and training, it is possible to end up in a situation where procedures and attitudes in a lab are so set in stone that much of the training you bring to a job will have to be thrown out the window. This is sometimes due to good reasons, i.e., standard operating procedures are in place for tracking, verification, and certification reasons, or a lab does a certain type of work and has really worked out the best ways to efficiently get the job done that leave little room for variation. Other times this can simply be a result of micromanagement and other less-noble reasons. In cases like these, it may be that what the lab REALLY wants is to train someone from the ground up, but they didn't really know that. It can be a shocker when it happens.
My point is that I would encourage you to get your degree and go for it. The microscopy world is full of wonderful and rewarding careers. But be prepared to be flexible and open to suggestion when joining a lab. My experience is that virtually everybody has their own, sometimes radically different, ways of doing things and many people don't accept change easily or quickly. Be prepared to blend in and as time goes on you may find that your ideas and training will be allowed to express themselves, or you may find that the people that hire you have better ideas that you can learn from. Or, and this is where it gets good, you will likely find that your new associates are interested in the new ideas and attitudes you bring to the lab, as well as passing along their own hard-won experience to you.
Best of luck, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: beadrysdale-at-yahoo.com [mailto:beadrysdale-at-yahoo.com] Sent: Wednesday, January 31, 2007 8:33 AM To: Tindall, Randy D.
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Title-Subject: [Filtered] Job outlook for microscopy technicians
Question: Hello. I am considering undertaking a two-year associate degree program to become a microscopy technician. I was wondering if anyone would be willing to comment on the job prospects I might have as new graduate with no science experience beyond this associate degree. The college's placement stats. look good but I would like a second opinion. Also, I am in my early forties and wonder if that would work against me finding a job (one of the draws of microscopy is that it is such a specialized skill that I would think employers would look beyond age). Thanks for any help you can give me.
Hi Bea, I'm always glad to see people go into microscopy, regardless of their age. And yes I believe employer looks beyond age as it take years to acquire the experience and background to be a general purpose microscopist. You must never stop learning. From this point on, is where I differ from most of the feed back I suspect you're going to get. It's just my experience and my opinion. Please send death threats off line to prevent soaking up the bandwidth.
In todays market I am seeing BSs hired to do the work that technicians did. I see MSs hired to do the job that chemists with BSs did 20 years ago. It appears that only PhDs count. This seem to be the trend in large companies, especial those with government contracts or grants. Many companies will assume that you can teach microscopy to anyone off the street because you're just lookin' at stuff. SEM/EDS/TEM/STEM/microtome, well they are computer run aren't they? Ever manager I know thinks they can interpret photomicrographs, especially after I annotate them.
I have seem incredible bright technicians, masters of their trade, people capable of good scientific, independent work locked into positions that they have outgrown and are actively working at a significantly higher level but frozen to title and pay grade (and privileges) because they don't have a BS or better.
I am and remain a microscopist because I love the work and windows to the world it opens for me. I have had the opportunity to meet and hob nob with some of the best. It's the best job in the world and I really love my current position, but I think you should realize that some opportunities and options will remain closed to you with only a two year degree, just as some remain closed with a BS or MS.
Stay safe............Frank
beadrysdale-at-yahoo .com To: frank.karl-at-degussa.com cc: 01/31/2007 09:32 Subject: [Microscopy] viaWWW: Outlook for microscopy technicians AM Please respond to beadrysdale
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Title-Subject: [Filtered] Job outlook for microscopy technicians
Question: Hello. I am considering undertaking a two-year associate degree program to become a microscopy technician. I was wondering if anyone would be willing to comment on the job prospects I might have as new graduate with no science experience beyond this associate degree. The college's placement stats. look good but I would like a second opinion. Also, I am in my early forties and wonder if that would work against me finding a job (one of the draws of microscopy is that it is such a specialized skill that I would think employers would look beyond age). Thanks for any help you can give me.
This reply is dry and not passionate - don't let that fool you, I really love microscopy and my job.
When you mention a lack of science background - are you talking about biological or electronics type of science? If you have no biological science experience, you could look for work in a clinical lab where protocols are pretty well established and have to be standardized. If the research field appeals to you, this may prove to be challenging and you may want to increase your biology background as much as possible - for example, to be able to interpret what you see in a microscope and figure out whether an image is 'real' and significant or whether the equipment needs a tune-up. It helps if you understand whatever you see.
These days of digital imaging and complex microscope systems, an understanding of the electronics is also an asset - make sure your course covers that adequately. Another area of microscopy is in the material sciences for which biology is not essential. This also requires some knowledge of different areas of science. Also, there are always jobs working for one of the microscope manufacturers or retailers.
The age factor is not an issue these days, competence is more important. There is always a need for this specialty and actually older people have more experience of having come across a particular problem or have done a specific technique - so keep your eyes open especially for troubleshooting tricks.
Let me know if you need any information, encouragement, whatever. Good luck. Judy
Title-Subject: [Filtered] Job outlook for microscopy technicians
Question: Hello. I am considering undertaking a two-year associate degree program to become a microscopy technician. I was wondering if anyone would be willing to comment on the job prospects I might have as new graduate with no science experience beyond this associate degree. The college's placement stats. look good but I would like a second opinion. Also, I am in my early forties and wonder if that would work against me finding a job (one of the draws of microscopy is that it is such a specialized skill that I would think employers would look beyond age). Thanks for any help you can give me.
Judy Trogadis Bio-Imaging Coordinator St. Michael's Hospital, 7Queen 30 Bond St. Toronto, ON M5B 1W8, Canada ph: 416-864-6060 x6337 pager: 416-685-9219 fax: 416-864-6043 trogadisj-at-smh.toronto.on.ca
==============================Original Headers============================== 14, 19 -- From trogadisj-at-smh.toronto.on.ca Wed Jan 31 10:23:30 2007 14, 19 -- Received: from smh.toronto.on.ca (mail.smh.toronto.on.ca [199.71.175.103]) 14, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0VGNTjs026960 14, 19 -- for {microscopy-at-microscopy.com} ; Wed, 31 Jan 2007 10:23:30 -0600 14, 19 -- Received: from ([172.27.12.43]) 14, 19 -- by beethoven.smh.toronto.on.ca with ESMTP id KP-GCT47.46619052; 14, 19 -- Wed, 31 Jan 2007 11:23:00 -0500 14, 19 -- Received: from SMTPOUT-MTA by beethoven.smh.toronto.on.ca 14, 19 -- with Novell_GroupWise; Wed, 31 Jan 2007 11:23:00 -0500 14, 19 -- Message-Id: {s5c07c14.028-at-beethoven.smh.toronto.on.ca} 14, 19 -- X-Mailer: Novell GroupWise Internet Agent 6.5.2 14, 19 -- Date: Wed, 31 Jan 2007 11:22:26 -0500 14, 19 -- From: "Judy Trogadis" {TrogadisJ-at-smh.toronto.on.ca} 14, 19 -- To: {Microscopy-at-microscopy.com} , {beadrysdale-at-yahoo.com} 14, 19 -- Subject: [Microscopy] Re: viaWWW: Outlook for microscopy technicians 14, 19 -- Mime-Version: 1.0 14, 19 -- Content-Type: text/plain; charset=US-ASCII 14, 19 -- Content-Transfer-Encoding: 7bit 14, 19 -- Content-Disposition: inline ==============================End of - Headers==============================
Thanks to all for your answers. I don't know why but the vast majority didn't take seriously the possibility that I may stop cutting to sign autographs. I will remember this ;-)
In a room with air conditioning, all doors closed and where all conditions are there to offer an optimal stable environment, I couldn't believe that temperature changes could have such effects. Apparently I was wrong, as most of you point this as the main cause, even if other obscure reasons have been proposed too (and even if I would tend to believe the second category more than the first one). It is strange to see how a so common technique which delivers such high quality data can be subject to the same common uncontrollable events. Just to be fair I'll finish by pointing out that my Leica ultramicrotome is near from perfection, it is a real delight to use and cannot be taken responsible for this problem.
Stephane
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One thing I might add to the comments you have already gotten is that you need to be prepared to relocate, Perhaps clear across the country. My last hire was living in California and came to work here in Florida for an entry level job. Second, lack of experience may be to your advantage since many hires are at entry level and folks with more experience are going to want more money. You are also going to find that jobs in the engineering area will pay higher than those in the biological area.
Greg Erdos University of Florida, Retired.
beadrysdale-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both beadrysdale-at-yahoo.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: beadrysdale-at-yahoo.com } Name: Bea Drysdale } } Organization: unaffiliated } } Title-Subject: [Filtered] Job outlook for microscopy technicians } } Question: Hello. I am considering undertaking a two-year associate degree program to become a microscopy technician. I was wondering if anyone would be willing to comment on the job prospects I might have as new graduate with no science experience beyond this associate degree. The college's placement stats. look good but I would like a second opinion. Also, I am in my early forties and wonder if that would work against me finding a job (one of the draws of microscopy is that it is such a specialized skill that I would think employers would look beyond age). Thanks for any help you can give me. } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 12 -- From zaluzec-at-microscopy.com Wed Jan 31 08:30:49 2007 } 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0VEUm8u012460 } 6, 12 -- for {microscopy-at-microscopy.com} ; Wed, 31 Jan 2007 08:30:49 -0600 } 6, 12 -- Mime-Version: 1.0 } 6, 12 -- X-Sender: (Unverified) } 6, 12 -- Message-Id: {p06110400c1e65873d8f2-at-[206.69.208.22]} } 6, 12 -- Date: Wed, 31 Jan 2007 08:30:46 -0600 } 6, 12 -- To: microscopy-at-microscopy.com } 6, 12 -- From: beadrysdale-at-yahoo.com (by way of MicroscopyListserver) } 6, 12 -- Subject: viaWWW: Outlook for microscopy technicians } 6, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 3, 27 -- From gwe-at-ufl.edu Wed Jan 31 11:30:39 2007 3, 27 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0VHUcUk018130 3, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 31 Jan 2007 11:30:38 -0600 3, 27 -- Received: from [10.228.0.96] (ssrb-vpn1-0-96.vpn.ufl.edu [10.228.0.96]) 3, 27 -- (authenticated bits=0) 3, 27 -- by smtp.ufl.edu (8.13.7/8.13.7/2.5.9) with ESMTP id l0VHUatn1224878; 3, 27 -- Wed, 31 Jan 2007 12:30:36 -0500 3, 27 -- Message-ID: {45C0D25C.4050509-at-ufl.edu} 3, 27 -- Date: Wed, 31 Jan 2007 12:31:08 -0500 3, 27 -- From: Greg Erdos {gwe-at-ufl.edu} 3, 27 -- Reply-To: gwe-at-ufl.edu 3, 27 -- User-Agent: Mozilla Thunderbird 1.0.7 (Windows/20050923) 3, 27 -- X-Accept-Language: en-us, en 3, 27 -- MIME-Version: 1.0 3, 27 -- To: beadrysdale-at-yahoo.com, 3, 27 -- "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com} 3, 27 -- Subject: Re: [Microscopy] viaWWW: Outlook for microscopy technicians 3, 27 -- References: {200701311431.l0VEVtwG013608-at-ns.microscopy.com} 3, 27 -- In-Reply-To: {200701311431.l0VEVtwG013608-at-ns.microscopy.com} 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- X-Greylist: Sender succeeded SMTP AUTH authentication, not delayed by milter-greylist-3.0rc3 (smtp.ufl.edu [128.227.74.56]); Wed, 31 Jan 2007 12:30:37 -0500 (EST) 3, 27 -- X-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 3, 27 -- X-UFL-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 3, 27 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 3, 27 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both iancozine-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: iancozine-at-gmail.com Name: Ian Cozine
Organization: MATC
Title-Subject: [Filtered] Job outlook for microscopy technicians
Question: Bea,
I am a 2nd year student at the EM program at MATC in Madison, Wisconsin and I graduate this May. While I can't add many informed comments about the outlook for technicians yet, I can say that I have thoroughly enjoyed the program. It is a small program so there is plenty of one on one instruction. We get to spend several hours a week in the lab, and from day one we are working with the instruments. I think this is one advantage over a B.S. degree. Upon graduating I will have 2 years working with all sorts of equipment and techniques and I think that most 4 year degrees aren't going to provide that much hands on experience.
I do know that the placement rates are quite good. If you graduate from the program and are reasonably proficient you will get a job. Our instructors do a really great job of locating job leads for us all over the country. One consideration, however, is that chances are you will be moving for your first job.
I would be hard-pressed to think of another 2 year program where upon graduation you will get to do such interesting work. I would not hesitate recommending a microscopy degree to anyone.
Title-Subject: [Filtered] Job outlook for microscopy technicians
Question: Hello. I am considering undertaking a two-year associate degree program to become a microscopy technician. I was wondering if anyone would be willing to comment on the job prospects I might have as new graduate with no science experience beyond this associate degree. The college's placement stats. look good but I would like a second opinion. Also, I am in my early forties and wonder if that would work against me finding a job (one of the draws of microscopy is that it is such a specialized skill that I would think employers would look beyond age). Thanks for any help you can give me.
Hello: I use a 3.2 Megapixel Digital camera PN OMCAM32 from The Microscope Store for OPTICAL microscopy with great results. The camera mounts thru the eyepiece and has an LCD so you can see exactly what you are getting. The brighter the light the better the image but I get images in dimmer light that I can use. The pictures are stored on an internal memory card which is then downloaded to a PC via cable. It requires no special software for download.
I talked to Eric Naff. The OMCAM32 is not listed on the web site, but the store will reply "yes" to an e-mail request regarding it.
The Microscope Store 316 Windy Pines Lane Rocky Mount, VA 24151 Voice (877) 409-3556 FAX (540) 489-4785 2rhonda-at-microscope-store.com www.microscope-store.com
I hope this helps,
John R Willis Tech Writer Valcor Eng Corp 2 Lawrence Rd Springfield, NJ 07081 Voice (973) 467-8400 X 7257 FAX (973) 467-8382 johnwillis-at-valcor.com www.valcor.com
==============================Original Headers============================== 6, 32 -- From johnwillis-at-valcor.com Wed Jan 31 14:45:47 2007 6, 32 -- Received: from mail.valcor.com (mail.valcor.com [12.30.108.82]) 6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l0VKjlWA011519 6, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 31 Jan 2007 14:45:47 -0600 6, 32 -- Received: from mail.valcor.com (localhost.localdomain [127.0.0.1]) 6, 32 -- by mail.valcor.com (8.13.7/8.13.4) with ESMTP id l0VKmG60026624 6, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 31 Jan 2007 15:48:17 -0500 6, 32 -- Received: from JohnWillis ([192.168.1.68])(authenticated bits=0)by 6, 32 -- mail.valcor.com (8.13.7/8.13.4) with ESMTP id l0VKli7W026491;Wed, 31 Jan 6, 32 -- 2007 15:47:55 -0500 6, 32 -- Message-ID: {024501c74578$b585b7e0$4401a8c0-at-valcor.local} 6, 32 -- From: "John Willis" {johnwillis-at-valcor.com} 6, 32 -- To: {Microscopy-at-microscopy.com} 6, 32 -- Cc: {johnwillis-at-valcor.com} 6, 32 -- Subject: Camera 6, 32 -- Date: Wed, 31 Jan 2007 15:45:04 -0500 6, 32 -- MIME-Version: 1.0 6, 32 -- Content-Type: text/plain; 6, 32 -- format=flowed; 6, 32 -- charset=iso-8859-1; 6, 32 -- reply-type=original 6, 32 -- Content-Transfer-Encoding: 7bit 6, 32 -- X-Priority: 3 6, 32 -- X-MSMail-Priority: Normal 6, 32 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3028 6, 32 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 6, 32 -- X-imss-version: 2.045 6, 32 -- X-imss-result: Passed 6, 32 -- X-imss-scanInfo: M:P L:E SM:0 6, 32 -- X-imss-tmaseResult: TT:0 TS:0.0000 TC:00 TRN:0 TV:3.6.1039(14870.003) 6, 32 -- X-imss-scores: Clean:57.20883 C:2 M:3 S:5 R:5 6, 32 -- X-imss-settings: Baseline:4 C:3 M:3 S:3 R:3 (1.0000 1.0000) ==============================End of - Headers==============================
Are there any ultrastructural differences between monocytes and monocyte-derived dendritic cells? I've been looking at marmoset monocytes, immature dendritic cells and matrure dendritic cells and am having difficulties differentiating between them at the ultrastructural level.
Are there any references available to view images of these cells?
Regards,
John Brealey EM Unit Queen Elizabeth Hospital Adelaide South Australia
==============================Original Headers============================== 6, 33 -- From john.brealey-at-imvs.sa.gov.au Wed Jan 31 19:12:51 2007 6, 33 -- Received: from adl0741.systems.sa.gov.au (adl0741.systems.sa.gov.au [143.216.236.20]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l111Cnln029344 6, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 31 Jan 2007 19:12:50 -0600 6, 33 -- Received: from adl0741.systems.sa.gov.au (localhost [127.0.0.1]) 6, 33 -- by adl0741.systems.sa.gov.au OUTGOING (8.12.10/8.12.10) with ESMTP id l111Clji025278 6, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 1 Feb 2007 11:42:47 +1030 (CST)' 6, 33 -- Received: from ablett.imvs.sa.gov.au (ablett.imvs.sa.gov.au [10.20.98.41]) 6, 33 -- by adl0741.systems.sa.gov.au INCOMING (8.12.10/8.12.10) with ESMTP id l111ClR6025275 6, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 1 Feb 2007 11:42:47 +1030 (CST)' 6, 33 -- Received: from localhost (mesh.imvs.sa.gov.au [10.20.98.37]) 6, 33 -- by ablett.imvs.sa.gov.au (Postfix) with ESMTP id 9A4AC34DA4 6, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 1 Feb 2007 11:42:47 +1030 (CST) 6, 33 -- X-Virus-Scanned: by amavisd-new at imvs.sa.gov.au 6, 33 -- Received: from ablett.imvs.sa.gov.au ([10.20.98.41]) 6, 33 -- by localhost (.imvs.sa.gov.au [10.20.98.37]) (amavisd-new, port 10024) 6, 33 -- with LMTP id 5Q4x0tFg1s63 for {Microscopy-at-MSA.Microscopy.Com} ; 6, 33 -- Thu, 1 Feb 2007 11:42:41 +1030 (CST) 6, 33 -- Received: from iqe36042 (iqepc200.imvs.sa.gov.au [10.20.138.200]) 6, 33 -- by ablett.imvs.sa.gov.au (Postfix) with SMTP id B38F234DB4 6, 33 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 1 Feb 2007 11:42:41 +1030 (CST) 6, 33 -- From: "John Brealey" {john.brealey-at-imvs.sa.gov.au} 6, 33 -- To: "Listserver" {Microscopy-at-MSA.Microscopy.Com} 6, 33 -- Subject: Ultratsructure of Dendritic Cells 6, 33 -- Date: Thu, 1 Feb 2007 11:42:40 +1030 6, 33 -- Message-ID: {000001c7459e$125729c0$c88a140a-at-iqe36042} 6, 33 -- MIME-Version: 1.0 6, 33 -- Content-Type: text/plain; 6, 33 -- charset="us-ascii" 6, 33 -- Content-Transfer-Encoding: 7bit 6, 33 -- X-Mailer: Microsoft Office Outlook 11 6, 33 -- Thread-Index: AcdFnflmKAUnplW8Rb6sBxBTxsNV+A== 6, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1807 ==============================End of - Headers==============================
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