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From: vladislav_speransky-at-nih.gov
Date: Thu, 1 Mar 2007 10:17:20 -0600
Subject: [Microscopy] opinions wanted on lab refrigerator

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Thanks to Roger, Steve Chapman and Bill Tivol for your replies - I do
seem to remember knowing at one time that the objective and selected
area apertures did opposite things when the objective lens is turned
off, but it has been a while..
I did find I can get strong contrast in low mag (LM) mode using the SA
aperture and have an acceptable field of view, although I couldn't get
dislocations to be visible at 1000x in LM mode when they are blindingly
obvious in standard mode at 2000x. Also a very significant increase in
brightness if I turn C1 down below it's normal minimum using free lens
control. Unfortunately, to see the dislocations I have to have a small
aperture in the diffraction plane, so it's no use using larger
apertures. So some more experimentation is needed, if I do get a good
setup usilg FLC I'm happy to pass the information on to anyone else who
finds they have the same problem.

Richard

________________________________________
Richard Beanland
Materials Analysis
Bookham
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________


-----Original Message-----
X-from: Roger Ristau [mailto:raristau-at-ims.uconn.edu]
Sent: 28 February 2007 16:37
To: Richard Beanland

Dear all,

Can anybody share their recent experience buying a new lab
refrigerator? One of ours, a rather old machine, just broke, and we
started looking up in the catalogs, so many choices there...
Maybe someone has recently done the research and would be willing to
share? I would appreciate specific comments.

This fridge is to be used for storing EM-related stuff, including
antibodies, in the fridge and in the freezer compartment. I do
realize, that people often want to avoid the models with auto
defrost, because those actually warm up for some periods. But I used
to think that putting the vials/tubes into one of those special
Nalgene containers would protect the stuff from melting? Is that
really true? Because if it is true, then it seems more convenient to
get an auto one. Any experience on that?

Thanks!
Vlad


________________________________________________
Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
DBEPS/ORS, National Institutes of Health
13 South Dr, Rm. 3N17 MSC 5766
Bethesda, MD 20892
301 496-3989
vladislav_speransky-at-nih.gov


==============================Original Headers==============================
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7, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov}
7, 22 -- Subject: opinions wanted on lab refrigerator
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From: rcmoretz-at-gmail.com
Date: Thu, 1 Mar 2007 10:49:49 -0600
Subject: [Microscopy] Re: opinions wanted on lab refrigerator

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Vladislav:

You do not want a standard refrigerator. It has to be explosion
proof, which automatically rules out auto defrost. And no, the Nalge
containers do not protect against the freeze/thaw cycle--particularly
bad if you are storing any biologicals.
One question--what temperature do you want/need your freezer to be?
-10C? -20C? We just recently got a Barnstead with the -20C freezer.
I think it's a monster, but it wasn't my decision.... There has been
a long discussion about the negatives for auto defrost freezers on the
histonet listserve. Google the listserve and search the archives. It
was a lively and thorough discussion.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals
Ridgefield, CT

On 3/1/07, vladislav_speransky-at-nih.gov {vladislav_speransky-at-nih.gov} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear all,
}
} Can anybody share their recent experience buying a new lab
} refrigerator? One of ours, a rather old machine, just broke, and we
} started looking up in the catalogs, so many choices there...
} Maybe someone has recently done the research and would be willing to
} share? I would appreciate specific comments.
}
} This fridge is to be used for storing EM-related stuff, including
} antibodies, in the fridge and in the freezer compartment. I do
} realize, that people often want to avoid the models with auto
} defrost, because those actually warm up for some periods. But I used
} to think that putting the vials/tubes into one of those special
} Nalgene containers would protect the stuff from melting? Is that
} really true? Because if it is true, then it seems more convenient to
} get an auto one. Any experience on that?
}
} Thanks!
} Vlad
}
}
} ________________________________________________
} Vlad Speransky, Staff Scientist
} Supramolecular Structure and Function Resource
} DBEPS/ORS, National Institutes of Health
} 13 South Dr, Rm. 3N17 MSC 5766
} Bethesda, MD 20892
} 301 496-3989
} vladislav_speransky-at-nih.gov
}
}
} ==============================Original Headers==============================
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} 7, 22 -- Subject: opinions wanted on lab refrigerator
} 7, 22 -- Date: Thu, 1 Mar 2007 11:15:52 -0500
} 7, 22 -- X-Mailer: Apple Mail (2.752.2)
} ==============================End of - Headers==============================
}

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From: dennis1231-at-yahoo.com
Date: Thu, 1 Mar 2007 11:00:04 -0600
Subject: [Microscopy] viaWWW: immunoEM antibody

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Email: dennis1231-at-yahoo.com
Name: Dennis McDaniel

Title-Subject: [Filtered] immunoEM antibody

Question: Hello,

I am doing some immunoEM work for another researcher, and so far none
of the antibodies we have tried have been successful. The researcher
would simply like to see the correct immunolocalization of anything
in order to validate the fixation and labeling methods we are using.

I am in search of a commercially available antibody suitable for
immunoEM in cells/tissues that have been fixed in 4%
formaldehyde/0.5% glutaraldehyde. Ideally, the antibody would raised
against an epitope that is present in most or all sections of most
cells and that is localized to structures that may be easily
discerned in cells that have not undergone optimal fixation (i.e. no
OsO4 post-fix). I am thinking of something like an antibody to a
mitochodrial or nuclear component. While I realize that no antibody
will work in all cells, I was hoping someone may know of an antibody
to a highly conserved protein that has worked in several different
cell types.

Any suggestions?


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From: phillipst-at-missouri.edu
Date: Thu, 1 Mar 2007 12:14:31 -0600
Subject: [Microscopy] Re: viaWWW: immunoEM antibody

Contents Retrieved from Microscopy Listserver Archives
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First you should realize getting one antibody to work on a tissue fixed and
embedded in a particular manner doesn't mean other antibodies will work in
a similar fashion. Second, you fail to mention what species and tissue you
are working on which makes suggestions of an antibody difficult. It is
often easiest to look for a Medline or PubMed search of your tissue and
species to see what antibodies have worked in EM immuno studies. Sometimes
I just search the Journal of Histochemistry & Cytochemistry for a
particular tissue since most of their papers do either LM or EM
immuno. good luck.



At 11:00 AM 03/01/07, you wrote:



} ----------------------------------------------------------------------------
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Thomas E. Phillips, PhD
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From: PWebster-at-hei.org
Date: Thu, 1 Mar 2007 12:29:37 -0600
Subject: [Microscopy] Re: viaWWW: immunoEM antibody

Contents Retrieved from Microscopy Listserver Archives
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Dear Dennis,

To assist you we really need more information. How are you attempting to
perform the immunolabeling? Are you applying the antibodies to
resin-embedded sections, cryosections or are you performing a pre-embedding
protocol?

When you say that the labeling you are doing doesn't work, do you mean that
the antibody is not labeling at all, is there too much label, or is the
label in the "wrong" place?

There are a very large number of antibodies that do label tissues after been
fixation using your fixative so it is easy to suspect that something is
going on with your specimen preparation.

Try this: fix your tissues with formaldehyde alone and prepare the specimens
for EM post-embedding (i.e. Labeling sections). Prepare semi-thin sections,
mount them on glass coverslips (or slides) and immunolabel for light
microscopy. You should be able to see a signal if you use fluorescent
secondaries on LR White or Lowicryl-embedded material and cryosectioned
specimens.

Contact me if you need more help.

Regards,

Paul Webster.




Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
(213) 273 8026
pwebster-at-hei.org


} From: {dennis1231-at-yahoo.com}
} Reply-To: {dennis1231-at-yahoo.com}
} Date: Thu, 1 Mar 2007 11:03:42 -0600
} To: {pwebster-at-hei.org}
} Subject: [Microscopy] viaWWW: immunoEM antibody
}
}
}
}
} ----------------------------------------------------------------------------
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} ---------------------------------------------------------------------------
}
} Email: dennis1231-at-yahoo.com
} Name: Dennis McDaniel
}
} Title-Subject: [Filtered] immunoEM antibody
}
} Question: Hello,
}
} I am doing some immunoEM work for another researcher, and so far none
} of the antibodies we have tried have been successful. The researcher
} would simply like to see the correct immunolocalization of anything
} in order to validate the fixation and labeling methods we are using.
}
} I am in search of a commercially available antibody suitable for
} immunoEM in cells/tissues that have been fixed in 4%
} formaldehyde/0.5% glutaraldehyde. Ideally, the antibody would raised
} against an epitope that is present in most or all sections of most
} cells and that is localized to structures that may be easily
} discerned in cells that have not undergone optimal fixation (i.e. no
} OsO4 post-fix). I am thinking of something like an antibody to a
} mitochodrial or nuclear component. While I realize that no antibody
} will work in all cells, I was hoping someone may know of an antibody
} to a highly conserved protein that has worked in several different
} cell types.
}
} Any suggestions?
}
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 9, 12 -- From zaluzec-at-microscopy.com Thu Mar 1 11:00:04 2007
} 9, 12 -- Received: from [172.16.1.63] (msdvpn8.msd.anl.gov [130.202.238.72])
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} 9, 12 -- From: dennis1231-at-yahoo.com (by way of MicroscopyListserver)
} 9, 12 -- Subject: viaWWW: immunoEM antibody
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==============================Original Headers==============================
15, 20 -- From PWebster-at-hei.org Thu Mar 1 12:29:36 2007
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15, 20 -- Subject: Re: [Microscopy] viaWWW: immunoEM antibody
15, 20 -- From: "Webster, Paul" {PWebster-at-hei.org}
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From: sarj0007-at-unf.edu
Date: Thu, 1 Mar 2007 16:48:55 -0600
Subject: [Microscopy] viaWWW: Drying out live cells

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Email: sarj0007-at-unf.edu
Name: Jason Saredy

Organization: University of North Florida

Title-Subject: [Filtered] Drying out live cells

Question: I have some cells in a MEM(mostly salts and amino acids and
the like) that I would like to image with our ESEM in environmental
mode. This is my first time trying to view live material and spent
all afternoon trying to slowly dry out the sample and ended up over
drying and lysing the cells. Does anyone with experience in this
area know what would be the optimal way to slowly dry the sample in
the chamber (ie what RH, how long, pressure)? Or perhaps a good
reference on how to do this? Thank you. And I apologize if this
went out three times, i tried emailing the listserv directly and it
kept bouncing back.



-Jason Saredy



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From: PWebster-at-hei.org
Date: Thu, 1 Mar 2007 17:01:22 -0600
Subject: [Microscopy] EMBO World Course 2007

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

In case anyone is interested, the European Molecular Biology Organization is
funding an electron microscopy practical course in Singapore this year.

There are no fees for the course but applicants are chosen on the quality of
their scientific proposals.

The web site provides more details than I can cover here.

http://cwp.embo.org/wpc07-04/

Regards,

Paul Webster.


Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
(213) 273 8026
pwebster-at-hei.org




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From: dcromey-at-email.arizona.edu
Date: Thu, 1 Mar 2007 17:36:24 -0600
Subject: [Microscopy] imaging membrane migration assays

Contents Retrieved from Microscopy Listserver Archives
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A colleague is doing migration assays using transwell plates. The
smaller transwells are darn near impossible to image because the wells
are both too deep for an upright scope and not deep enough for an
inverted. I suppose we could cut the membranes out of the wells and
mount them on slides, but I seem to remember that they have the nasty
habit of rolling up when you cut them out.

Does anyone have any good tricks/tips for imaging these membranes? Is
there a mounting media with an RI that will make the membrane almost
disappear?

Thanks,
Doug

I asked what brand plates they were using and the response was:

BD Falcon* Cell Culture Companion Plates for Inserts - 24well
BD Falcon* Cell Culture Inserts

--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Douglas W. Cromey, M.S. - Assistant Scientific Investigator
Dept. of Cell Biology & Anatomy, University of Arizona
1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA

office: AHSC 4212 email: Cromey-at-Arizona.edu
voice: 520-626-2824 fax: 520-626-2097

http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"


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From: phillipst-at-missouri.edu
Date: Thu, 1 Mar 2007 17:53:09 -0600
Subject: [Microscopy] Re: viaWWW: Drying out live cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I am not 100% clear on your protocol but if you dry out cells in MEM, you
are exposing them to a high osmotic buffer in the process and therefore it
would be surprising if they didn't lyse. The water will evaporate but the
salts simply concentrate.


At 04:50 PM 03/01/07, you wrote:



} ----------------------------------------------------------------------------
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9, 21 -- Subject: Re: [Microscopy] viaWWW: Drying out live cells
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From: musselmannk-at-mail.nih.gov
Date: Fri, 2 Mar 2007 00:15:23 -0600
Subject: [Microscopy] \viaWWW: Fluorescence staining of frozen sections

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Email: musselmannk-at-mail.nih.gov
Name: Kurt Musselmann

Organization: NIH/NIDCR

Title-Subject: [Filtered] Fluorescence staining of frozen sections

Question: Hi all

I am trying to do fluorescence staining of frozen sections (10 um
thick). Do I have to include a step in which I wash the OCT off the
slides? or will fixing them in acetone do?

Alternatively, can I just fix the tissue in ethanol and proceed in
the same fashion that I would for normal staining? (H&E, for
example?). (ethanol, then wash with water to get rid of the OCT,
then staining).

Thanks

Kurt


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From: David.Llewellyn-at-anu.edu.au
Date: Fri, 2 Mar 2007 00:15:58 -0600
Subject: [Microscopy] viaWWW: Equipment Wanted

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Email: David.Llewellyn-at-anu.edu.au
Name: David Llewellyn

Organization: Australian National University

Title-Subject: [Filtered] Equipment Wanted

Question: Would anyone have for sale any TEM Xsection prep.
equipment? wanted to start up a new lab. Ion Beam
mill,polishers,dimplers etc.

---------------------------------------------------------------------------

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From: Elliott-at-arizona.edu
Date: Fri, 2 Mar 2007 11:31:17 -0600
Subject: [Microscopy] Inkjet printers for EM, LM, Confocal, or Scientific Photography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi John
Thank you for that info. I did not know about turning off the
printer to decrease the ink drying question.
David


On Mar 2, 2007, at 10:00 AM, John Mackenzie wrote:

}
} David:
}
} Yes and no. The C80 series is fairly stable but needs to be exercised
} once a week. There are two things that are worth noting. The printer
} retracts the ink when the printer is turned off USING THE BUTTON ON
} THE
} TOP. (Using a strip to kill power does not) If you leave the
} printer on
} all the time the ink is sitting at the ready and dries out faster. The
} Epson system although the best does suffer from this problem. My
} C80 was
} more prone to this problem than the current C88. The C88 plus is so
} fast
} that it is worth the $80 to replace even a C88 let alone a C80. My
} advice would be to use the ink you have then upgrade (For about the
} cost
} of inks you get new printer). It is REALLY fast.
}
} This info might be useful to others so if it is ok with you, you might
} want to post it to the web.
}
}
}
} John
}
}
}
} David Elliott wrote:
} } Hi John
} }
} } I have a question about inkjet printers. I have a C80 that does
} } fine when it is used regularly, but if it is not used for a few
} } days the ink jets clog up and much ink is wasted cleaning the
} } print head.
} } Is this the same for all inkjets? Is there something that I can
} } do to keep my underused printers working?
} }
} } Thank you
} } David
} }
} }
} } On Feb 26, 2007, at 12:00 PM, john_mackenzie-at-ncsu.edu wrote:
} }
} } }
} } }
} } }
} } } --------------------------------------------------------------------
} } } --------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society
} } } of America
} } } To Subscribe/Unsubscribe -- http://www.microscopy.com/
} } } MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } --------------------------------------------------------------------
} } } --------
} } }
} } } Hi all:
} } }
} } } The needs of scientific digital imaging are not the same as they
} } } are for
} } } photography. We have done extensive testing of the printers and
} } } it boils
} } } down to two inkjets and one Laserjet
} } }
} } } The Epson C88 plus.. The best printer in the world for printing
} } } on plain
} } } paper C88 plus for a whopping $88.00 Nothing competes with it on
} } } plain
} } } paper. Total cost of a color print $0.17 with ink full coverage
} } }
} } } The best printer for black and white printing, and color
} } } printing on
} } } special papers is the Epson R2400. $700-800 range.
} } }
} } } The inks that Epson uses are very, very specialized and PATENTED.
} } } All
} } } "third party" inks are grossly inferior. ( Yes we have tested
} } } several
} } } but it requires purchasing flushing cartridges to switch so we
} } } rarely do
} } } it now)
} } }
} } } We really were impressed with the prints that John Cone was able to
} } } produce.( Piezography B/W )They were striking HOWEVER the print
} } } driver is seriously flawed
} } } and will not give you results that are scientifically valid. The
} } } new R2400 has
} } } a dedicated K3 mode that beats the Cone system running away.It is
} } } scientifically valid. I have both The Cone idea of using
} } } multiple blacks is a
} } } good one but Epsons implementation is the only one to use. Epson
} } } has added
} } } a B/W Tint mode that allows you to fine tune the BW so that it
} } } looks just like
} } } Agfa Boviera prints Longevity 200 years
} } }
} } } An 8 x 10 B/W print was $2 to $3 many years ago . If you actually
} } } do the
} } } experiment (which photographers do not) an 8 x 11 print on Premium
} } } photo glossy paper with full coverage is $0.50 for the paper and
} } } $0.15
} } } for the ink. If you use inferior ink with no tested longevity and
} } } unknown fading characteristics you might save $0.10. This makes
} } } no sense
} } } to me.( We by the way use our darkroom to store inks and paper)
} } }
} } } We do most of our work prints on our HP Laserjet in high resolution
} } } graphics mode (2400 dpi, 150 lpi ) at 2 cents a page (including
} } } toner).
} } } Also because the resolution is three times the advertised
} } } resolution,
} } } the you can't use refilled cartridges here either
} } }
} } } If you need a and a better print then use the c88. Save the best
} } } for
} } } the R2400. The three printers cost less than $2000. All
} } } computers have
} } } at least these three.
} } }
} } } In my workshops and my classes we spend 3-5 hours on this very
} } } subject.
} } } I know it is not simple and that this information is mainly the
} } } bullet
} } } conclusions.
} } }
} } } There are actually several other reasons for never using refilled
} } } inks
} } } or toners bottom line is that it is a bad practice and we never
} } } do it.
} } } We always use Epson papers for inkjets and we use Hammermill
} } } Laser for
} } } Lasrjets. (Sometimes Epson Bright white which costs the same)
} } }
} } } John
} } }
} } } --John Mackenzie, Jr.
} } } Coordinator for the Center for Electron Microscopy
} } } Professor of Microbiology
} } } North Carolina State University
} } } Phone (919) 515-2664 Fax (919) 515-8293
} } }
} } }
} } }
} } } ==============================Original
} } } Headers==============================
} } } 15, 19 -- From john_mackenzie-at-ncsu.edu Mon Feb 26 12:56:34 2007
} } } 15, 19 -- Received: from uni05mr.unity.ncsu.edu
} } } (uni05mr.unity.ncsu.edu [152.1.224.164])
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} } } Nv5.2006.1109) with ESMTP id l1QIuWM1007166
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} } } 13:56:33 -0500 (EST)
} } } 15, 19 -- Message-ID: {45E32D65.9070004-at-ncsu.edu}
} } } 15, 19 -- Date: Mon, 26 Feb 2007 13:56:37 -0500
} } } 15, 19 -- From: John Mackenzie {john_mackenzie-at-ncsu.edu}
} } } 15, 19 -- User-Agent: Thunderbird 1.5.0.9 (Windows/20061207)
} } } 15, 19 -- MIME-Version: 1.0
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} } } 15, 19 -- Subject: Re: Inkjet printers for EM, LM, Confocal, or
} } } Scientific Photography
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} } } format=flowed
} } } 15, 19 -- Content-Transfer-Encoding: 7bit
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} } } 15, 19 -- X-Spam-Level: IIIIIII
} } } ==============================End of -
} } } Headers==============================
} }
}
} --
} John M. Mackenzie Jr., PhD
} Professor of Microbology
} Coordinator, Center for Electron Microscopy
} Phone 919-515-2664 Fax 919-515-8293
}
}


==============================Original Headers==============================
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From: dac-at-research.umass.edu
Date: Fri, 2 Mar 2007 12:11:19 -0600
Subject: [Microscopy] Source of supplies for Balzers/Baltec (FF, evaporators)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I know that Baltec has changed distribution in the last year or so. I
was recently quoted a price for oscillator crystals of 2x what I had
paid to the previous supplier. Fortunately I located some old stock
(they were stored well, silver is not oxidized...) at the previous
pricing. Double is quite a price jump.

Is there an alternative to these doubled prices? Does anyone using
Baltec supplies have suggestions for supplies and support that haven't
taken such a steep jump. We have a Freeze-Fracture and a turbo-pumped
evaporator unit, and use the usual supplies for E-beam evaporators, film
thickness monitors, and the like.

Thanks,

Dale Callaham

==============================Original Headers==============================
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From: togo-at-uvic.ca
Date: Fri, 2 Mar 2007 13:19:37 -0600
Subject: [Microscopy] LM video cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We are looking at putting new video cameras on our teaching microscopes and
would like to be able to use as much of the resolution of the data
projectors in our classrooms as possible. In the past we've used small CCD
NTSC cameras that were intended for security purposes, but now we'd like
to be able to get something in the order of 1280 by 1024 pixels onto the
screen.

We'd be very grateful for suggestions of what other users have found worked
well for this purpose. Thanks for your thoughts.
_____________________________________
Tom Gore | Advanced Imaging Laboratory
Department of Biology | University of Victoria
Box 3020 Station CSC
Victoria BC V8W 3N5 Canada
voice 250 721 7134 fax 250 721 7120
web: http://web.uvic.ca/ail/


==============================Original Headers==============================
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From: microscopytoday-at-tampabay.rr.com
Date: Fri, 2 Mar 2007 13:22:06 -0600
Subject: [Microscopy] Microscopy Today Table of Contents March 2007

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

Here is the March 2007 Microscopy Today table of contents. I will close
the subscription list for this issue on Wednesday, Mar. 7th, 2007.

Microscopists in North America and MSA members anywhere can subscribe
for free. Anyone else may subscribe for US$50 per year (to PARTIALLY
cover postage). All subscriptions at http://www.microscopy-today.com
Thank you.

Ron Anderson, Editor
================================
Microscopy Reveals Early Neolithic Dentistry
Stephen W. Carmichael, Mayo Clinic

Microscopic Analysis of Metal Recovered from the Wreck of RMS Titanic
J.J. Hooper McCarty1 and T. Foecke1,2, 1The Johns Hopkins University,
Baltimore, MD, 2NIST, Gaithersburg, MD

STEM Imaging of Lattice Fringes and beyond in a UHR In-Lens
Field-Emission SEM
Vinh Van Ngo, Mike Hernandez, Bill Roth, and David C Joy,* Hitachi High
Technologies America, Inc., *University of Tennessee, Knoxville, TN

From the McArthur to the Millennium Health Microscope (MHM): Future
Developments in Microscope Miniaturization for International Health
Keith Dunning1 & J. Russell Stothard2, 1Dunning Associates, Bedford,
UK., 2Natural History Museum, London, UK

A Novel Technique of Hair Removal to Examine the Cuticle of Arthropods
B. N. Philip and C. Shillington, Eastern Michigan University, Ypsilanti, MI

Site Specific Three-dimensional Structural Analysis in Tissues and Cells
Using Automated DualBeam Slice &View
Ben Lich, FEI Company, Eindhoven, The Netherlands

Feature Characterization of Microfluidic Channels Created Using Direct
Laser Ablation
John Little* and Dan Borah,** *Hyphenated Systems, LLC, Burlingame, CA,
**University of Mass., Dartmouth, MA

Negatice Stiffness Vibration Isolation Technology for Nanotechnology
David L. Platus, Minus K Technology, Inc., Inglewood, CA

Improving Membrane Staining of Cultured Cells Using Ferrocyanide as a
Post-Fixative
Stphane Nizet

The Preparation of Mg, Cd and Zn Samples for Crystal Orientation Mapping
with BKD in an SEM
R.A. Schwarzer, Clausthal Univ. of Technology, Clausthal-Zellerfeld, Germany

Visualisation of Native Surfaces by Two-Step Molding
Stanislav N. Gorb, Max Planck Inst. for Metals Research, Stuttgart, Germany

Cold Temperature Preparation of XTEM Specimens of Embedded Metallic
Nanoparticles
Bernt Johannessen, David J. Llewellyn, Patrick Kluth, and Mark C.
Ridgway, Australian National University, Canberra, Australia

Industry News
NetNotes
SAMPLE PREPARATION - frozen tissue for TEM
SAMPLE PREPARATION - fixation of whole mosquitoes
SAMPLE PREPARATION - fixation for mitochondria
SAMPLE PREPARATION - fixation of mouse brain tissue
SAMPLE PREPARATION - purpose of PVP
SAMPLE PREPARATION - maleate buffers
SAMPLE PREPARATION - Vibratome sections
SAMPLE PREPARATION - LR White polymerization problem
SAMPLE PREPARATION SEM of cells without critical point drying
SAMPLE PREPARATION - SEM of cells in monolayer
SAMPLE PREPARATION - SEM of Zebra fish
SAMPLE PREPARATION - critical point drying
MICROTOMY - cryo-ultramicrotomy
MICROTOMY - specimen advance
LM - S waves in phase contrast optics
LM - 3-D reconstruction from serial paraffin sections
MICROSCOPY - LASIK, floaters, and posterior vitreous detachment
TEM - defect density threshold
TEM - effect of high temperature on grids
TEM - cleaning a LaB6 emitter
TEM - power line and stray field shielding
SEM - solid state versus a scintillator
SEM - low vs. high vacuum mode
SEM - beam penetration
EBL/ PMMA Mask

Index of Advertisers


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From: gary-at-gaugler.com
Date: Fri, 2 Mar 2007 14:08:56 -0600
Subject: [Microscopy] Re: LM video cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

If you find something like this, that would be
interesting to know. The only ones I know of
are not composite output but rather separate
colors and sync and require a control box and
special monitor (i.e., pricey).

By definition, NTSC is 768x494 and usually winds
up being 640x480.

gary g.


At 11:21 AM 3/2/2007, you wrote:




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From: r-holdford-at-ti.com
Date: Fri, 2 Mar 2007 14:53:18 -0600
Subject: [Microscopy] Re: LM video cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I like the Jenoptik ProgRes series of cameras
(http://www.jenoptik-los.de/cms.php?pageid=707&lang=1). Admittedly,
I've never tried projecting my data but it looks good on the computer
screen, especially with the CapturePro software that comes with the
camera. We have the FireWire models and use them in materials research.

togo-at-uvic.ca wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} We are looking at putting new video cameras on our teaching microscopes and
} would like to be able to use as much of the resolution of the data
} projectors in our classrooms as possible. In the past we've used small CCD
} NTSC cameras that were intended for security purposes, but now we'd like
} to be able to get something in the order of 1280 by 1024 pixels onto the
} screen.
}
} We'd be very grateful for suggestions of what other users have found worked
} well for this purpose. Thanks for your thoughts.
} _____________________________________
} Tom Gore | Advanced Imaging Laboratory
} Department of Biology | University of Victoria
} Box 3020 Station CSC
} Victoria BC V8W 3N5 Canada
} voice 250 721 7134 fax 250 721 7120
} web: http://web.uvic.ca/ail/
}
}
} ==============================Original Headers==============================
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} 3, 19 -- Subject: LM video cameras
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--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


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From: TindallR-at-missouri.edu
Date: Fri, 2 Mar 2007 15:17:07 -0600
Subject: [Microscopy] Dryer for TEM films?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers,

By some happy chance, might somebody in Listland have surplus film
drying cabinet? We are making room for a new microscope and need to get
rid of our BIG film dryer and hope to replace it with one of a size more
suited to the new world of digital micrography----i.e., much smaller.

We would be happy to pay shipping and a reasonable price for a
dust-discouraging dryer, ideally with a fan.

Thanks,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


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From: mager-at-interchange.ubc.ca
Date: Fri, 2 Mar 2007 15:54:37 -0600
Subject: [Microscopy] LM video cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Tom,
I just purchased a Motic (www.motic.com) microscope camera to replace the
old Pixera camera that died. These are not video cameras, but frame capture
cameras that capture a 1.3 megapixel colour image directly into a computer
over about 10 seconds. The focus mode is a faster scan, but not as high
resolution. The whole kit was only $430 CAD and included about five adapters
to fit the camera to the light microscope, all cables and software. I think
you will have a problem finding a high-resolution video camera and it might
be very expensive.
Having said that, you can now purchase a consumer HD video camera for less
than $1000. If it has a live output to the computer and you can make an
adaptor to fit it to the microscope, it should work. If you don't really
need video capture speeds, you will get more for less money with a frame
capture system.
My two pixels worth.
Regards,

Mary Mager
Electron Microscopist
Materials Eng. UBC
#309 - 6350 Stores Road
Vancouver, B.C. V6T 1Z4
Canada
Tel: 604-822-5648
Fax: 604-822-3619
email: mager-at-interchange.ubc.ca

-----Original Message-----
X-from: togo-at-uvic.ca [mailto:togo-at-uvic.ca]
Sent: March 2, 2007 11:25 AM
To: mager-at-interchange.ubc.ca

We are looking at putting new video cameras on our teaching microscopes and
would like to be able to use as much of the resolution of the data
projectors in our classrooms as possible. In the past we've used small CCD
NTSC cameras that were intended for security purposes, but now we'd like
to be able to get something in the order of 1280 by 1024 pixels onto the
screen.

We'd be very grateful for suggestions of what other users have found worked
well for this purpose. Thanks for your thoughts.
_____________________________________
Tom Gore | Advanced Imaging Laboratory
Department of Biology | University of Victoria
Box 3020 Station CSC
Victoria BC V8W 3N5 Canada
voice 250 721 7134 fax 250 721 7120
web: http://web.uvic.ca/ail/


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From: kraftpiano-at-gmail.com
Date: Fri, 2 Mar 2007 17:10:39 -0600
Subject: [Microscopy] SEM EBIC module & Carbon accessory extra to my needs. Trade?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I have an ISI ABT-SX40A SEM EBIC module, including the following:

EMF-II IE Amp (Unit from inside the chamber)

NIIOREOIP option board (Controller for the EBIC Image contrast)

connecting cables to connect into an AMS-110 option box.

All was working when microscope was decomissioned. I will include the
control module box if necessary, but if you don't need it, let me know.

I also have an extra Denton Desk II Carbon Accessory. I don't have the
sputter coater, so this is extra to my needs.

I have no use for these, but if you would like them, let me know what you
have to trade.

I run a non-profit organization which allows middle and high school students
to submit research proposals and complete research using our lab facility.

I would really like some kind of digital imaging system (Any kind that will
work on an older analog SEM), backscattered electron detector, or a sputter
coater.


Thanks,

Justin A. Kraft
Director
Center for Inquiry-Based Science Education

==============================Original Headers==============================
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12, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com}
12, 26 -- To: Microscopy-at-microscopy.com
12, 26 -- Subject: SEM EBIC module & Carbon accessory extra to my needs. Trade?
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From: gwe-at-ufl.edu
Date: Sat, 3 Mar 2007 17:57:05 -0600
Subject: [Microscopy] Microsopy Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

} ASSISTANT PROFESSOR MICROBIOLOGY AND CELL SCIENCE
}
} and
}
} DIRECTOR of ELECTRON MICROSCOPY and BIOIMAGING LABORATORY in the
INTERDISCIPLINARY CENTER FOR BIOTECHNOLOGY RESEARCH (ICBR)
}
}
} The Department of Microbiology and Cell Science, in partnership with
the Interdisciplinary Center for Biotechnology Research (ICBR) at the
University of Florida, is seeking a dynamic individual for a tenure
accruing faculty position and the head of an electron microscopy and
bioimaging core laboratory.
}
} The successful candidate will be expected to develop and maintain a
strong, externally-funded research program in microbiology or cell
science, participate in teaching, and direct the Electron Microscopy and
Bioimaging Laboratory (EMBL) within UFs ICBR, Technical support staff
will be provided to perform the daily service tasks of this ICBR
research facility. As the director, the incumbent will be charged with
setting the vision and acquiring the resources to make the ICBR EMBL
among the best in the nation. Candidates with interests in applying
modern bioimaging technology, including electron microscopy, to
important problems in cellular or molecular biology are especially
encouraged to apply.
}
}
} This is a 12-month tenure-accruing position that has 50% research,
25% service, and 25% instructional components. This assignment may
change in accordance with the needs of the units. Instructional
responsibilities will be to participate in teaching undergraduate and
graduate courses. The faculty member will also supervise graduate
student theses and dissertations.
}
}
} Required qualifications are a Ph.D. degree in cell biology or related
field, at least two years of postdoctoral research experience, and a
significant record of productivity, as demonstrated through refereed
publications, including published productivity in bioimaging.
}
} Salary and startup package will be competitive.
}
}
} To apply, submit a cover letter, curriculum vitae, a statement of
research and teaching interests in one electronic file to the email
below. Request official transcripts of your graduate academic work to be
sent directly from institution to the address below. Also request that 3
referees send letters of recommendation to the email below and a signed
copy of the letter to the address below. Review of applications will
begin March 15, 2007. Nominations of candidates are encouraged. Women
and minorities are encouraged to apply. Please forward all
applications, nominations and inquiries to:
}
}
} Dr. Peter Kima
}
} Chair, Search and Screen Committee
}
} Department of Microbiology and Cell Science
}
} University of Florida, PO Box 110700, Gainesville, FL 32611-0700
}
} Phone: (352)392-0384; Fax: (352)392-5922. E-mail: pkima-at-ufl.edu
}
}
} BACKGROUND INFORMATION: The Department of Microbiology and Cell
Science is an academic unit in the College of Agricultural and Life
Sciences within the Institute of Food and Agricultural Sciences (IFAS)
of the University of Florida (http://microcell.ufl.edu). It has well
established and extramurally funded programs in the areas of immunology,
microbial genetics, biochemistry, and physiology, as well as
host-parasite relationships, innate immunity, and biotechnology. The
Department offers B.S., M.S. and Ph.D. degrees through the College of
Agricultural and Life Sciences; the undergraduate degree is also offered
through the College of Liberal Arts and Sciences. Research programs are
sponsored through the Florida Agricultural Experiment Station. The
University of Florida, a land-grant university and member of the
Association of American Universities, enrolls approximately 48,000
students in seventeen academic units, including the Colleges of
Agricultural and Life Sciences, Dentistry, Engineering, Liberal Arts and
Sciences, Medicine, and Veterinary Medicine, and the School Of Natural
Resources and Environment.
}
}
} The ICBR is a campus-wide center that provides state-of-the-art
scientific expertise, training, instrumentation, and technologies to
faculty, staff, graduate students, and other research partners
throughout the university, state and nation (www.biotech.ufl.edu). The
ICBR Core Laboratories, including the Electron Microscopy Bioimaging
Laboratory, are staffed by ICBR personnel who are expert in each of the
research laboratory technologies (genomics, proteomics, sequencing, gene
expression, mass spectrometry, microarrays, hybridoma, flow cytometry,
electron microscopy, molecular biomarkers, genetic analysis, and
bioinformatics). The staff also advises and offers scientific and
technical consultation for investigators, and is strongly committed to
developing new advances in their technology areas and to passing this
knowledge on to faculty users.
}
} The University is located in Gainesville, Florida, a community with a
population of approximately 150,000 people in the metropolitan area,
located in North Central Florida, approximately mid-way between the
Atlantic Ocean and the Gulf of Mexico.
}


--
Greg Erdos
University of Florida, Retired
Micanopy, Florida

==============================Original Headers==============================
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From: nizets2-at-yahoo.com
Date: Sun, 4 Mar 2007 13:33:37 -0600
Subject: [Microscopy] mosquito by SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

A friend of mine needs a picture of a mosquito by SEM
to illustrate a presentation about mosquitoes.
Does anyone knows where to find good quality pictures
for free?

Best regards,

Stephane



____________________________________________________________________________________
Get your own web address.
Have a HUGE year through Yahoo! Small Business.
http://smallbusiness.yahoo.com/domains/?p=BESTDEAL

==============================Original Headers==============================
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6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com}
6, 19 -- Subject: mosquito by SEM
6, 19 -- To: microscopy-at-microscopy.com
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From: henriks-at-cox.net
Date: Sun, 4 Mar 2007 14:02:32 -0600
Subject: [Microscopy] mosquito by SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Stephane:

Try www.scharfphoto.com.

Alternatively, you can do a Google image search to see what happens.

Good luck.

David Henriks
Henriks-at-cox.net

-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Sunday, March 04, 2007 11:43 AM
To: Henriks-at-cox.net

Dear colleagues,

A friend of mine needs a picture of a mosquito by SEM
to illustrate a presentation about mosquitoes.
Does anyone knows where to find good quality pictures
for free?

Best regards,

Stephane



____________________________________________________________________________
________
Get your own web address.
Have a HUGE year through Yahoo! Small Business.
http://smallbusiness.yahoo.com/domains/?p=BESTDEAL

==============================Original Headers==============================
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6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com}
6, 19 -- Subject: mosquito by SEM
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==============================Original Headers==============================
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17, 26 -- Subject: RE: [Microscopy] mosquito by SEM
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From: dac-at-research.umass.edu
Date: Sun, 4 Mar 2007 22:05:58 -0600
Subject: [Microscopy] Re: Fw: Source of supplies for Balzers/Baltec (FF, evaporators)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Well, I don't think my weekend would have been complete without the
"wisdom" and nasty-toned comments of Mr. Smith. As a simple technician
trying to hold the line on costs for our clients, I think of information
exchange via the web as a very good basis for letting the "free-market"
operate. It worked wonders in remote villages of India where access to
telephones allowed poor farmers to get proper competitive prices for
their crops. And maybe I'm really out of touch, but salaries and
benefits in my neighborhood have been doing a lot of things BESIDES
keeping up.

I wish JERRY SMITH well in his search for full actualization using both
UPPER and lower case character coding.......

Dale Callaham

JERRY SMITH wrote:
}
} ----- Original Message ----- From: "JERRY SMITH" {JSMIT51-at-tampabay.rr.com}
} To: "JERRY SMITH" {JSMIT51-at-tampabay.rr.com}
} Sent: Saturday, March 03, 2007 10:32 AM
} Subject: Re: [Microscopy] Source of supplies for Balzers/Baltec (FF,
} evaporators)
}
}
} } BUT SUPPLIES HAVE ALWAYS BEEN KIND OF ERRATICALLY PRICED, SOMETIMES NOT
} } INCREASING FOR 6 YEARS AND THEN GOING CONSIDERABLY HIGHER, I GUESS
} } THAT IS THE FREE MARKET SYSTEM
} } ----- Original Message ----- From: "JERRY SMITH"
} } {JSMIT51-at-tampabay.rr.com}
} } To: {dac-at-research.umass.edu}
} } Sent: Saturday, March 03, 2007 10:26 AM
} } Subject: Re: [Microscopy] Source of supplies for Balzers/Baltec (FF,
} } evaporators)
} }
} }
} } } I GUESS THEY THINK ONLY THEIR SALARIES
} } } AND BENEFITS GO UP AND EVERYONE
} } } ELSE SHOULD STICK TO THE OLD PRICE FOR A HUNDRED YEARS
} } } ----- Original Message ----- From: {dac-at-research.umass.edu}
} } } To: {JSMIT51-at-TAMPABAY.RR.COM}
} } } Sent: Friday, March 02, 2007 1:12 PM
} } } Subject: [Microscopy] Source of supplies for Balzers/Baltec (FF,
} } } evaporators)
} } }
} } }
} } } }
} } } }
} } } }
} } } } ----------------------------------------------------------------------------
} } } }
} } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } } } America
} } } } To Subscribe/Unsubscribe --
} } } } http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } } ----------------------------------------------------------------------------
} } } }
} } } }
} } } } Hi all,
} } } }
} } } } I know that Baltec has changed distribution in the last year or so. I
} } } } was recently quoted a price for oscillator crystals of 2x what I had
} } } } paid to the previous supplier. Fortunately I located some old stock
} } } } (they were stored well, silver is not oxidized...) at the previous
} } } } pricing. Double is quite a price jump.
} } } }
} } } } Is there an alternative to these doubled prices? Does anyone using
} } } } Baltec supplies have suggestions for supplies and support that haven't
} } } } taken such a steep jump. We have a Freeze-Fracture and a turbo-pumped
} } } } evaporator unit, and use the usual supplies for E-beam evaporators,
} } } } film
} } } } thickness monitors, and the like.
} } } }
} } } } Thanks,
} } } }
} } } } Dale Callaham
} } } }
} } } } ==============================Original
} } } } Headers==============================
} } } } 5, 19 -- From dac-at-research.umass.edu Fri Mar 2 12:11:18 2007
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From: amich-at-ufl.edu
Date: Mon, 5 Mar 2007 13:45:14 -0600
Subject: [Microscopy] SEM of bacterial adhesion to soft contact lenses

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Dear Servers,

I would like to assess the level of bacterial adhesion to the soft
contact lenses surface with the SEM. Unfortunately I don?t have an
access to environmental mode. Could anyone suggest the reliable
approach to dehydrate soft contact lens without compromising its
surface?
Thank you in advance,

Albina


--
MIKHAYLOVA,ALBINA, PhD


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From: twigg-at-estd.nrl.navy.mil
Date: Tue, 6 Mar 2007 08:32:00 -0600
Subject: [Microscopy] viaWWW: Electrically Insulating Lacqer or Paint

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Name: Mark Twigg

Organization: NRL

Title-Subject: [Filtered] Electrically Insulating Lacqer or Paint

Question: I am looking for an electrically-insulating lacquer or paint that does not need to be baked or heated in order to adhere to a metal surface.

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From: dac-at-research.umass.edu
Date: Tue, 6 Mar 2007 08:59:45 -0600
Subject: [Microscopy] Re: viaWWW: Electrically Insulating Lacqer or Paint

Contents Retrieved from Microscopy Listserver Archives
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You haven't stated the full details of your application so my suggestion
may be useless. Does this need to go into a vacuum? Temperature range?
Voltage you are insulating? Resistivity required?

I would suggest looking into the compounds used for insulating HV
connections to avoid breakdown; generically these are called DAG or
Electro-DAG; electronics supply houses or Amateur Radio suppliers have it.

Or check these for higher tech possibilities:
http://www.cotronics.com/vo/cotr/
http://www.masterbond.com/

I have no interest in these companies....

Dale Callaham

twigg-at-estd.nrl.navy.mil wrote:
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} Title-Subject: [Filtered] Electrically Insulating Lacqer or Paint
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} Question: I am looking for an electrically-insulating lacquer or paint that does not need to be baked or heated in order to adhere to a metal surface.
}
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} ==============================Original Headers==============================
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==============================Original Headers==============================
6, 21 -- From dac-at-research.umass.edu Tue Mar 6 08:59:45 2007
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From: werner-at-rosharon.oilfield.slb.com
Date: Tue, 6 Mar 2007 09:21:24 -0600
Subject: [Microscopy] Re: viaWWW: Electrically Insulating Lacqer or Paint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Try http://www.glyptal.com/ for glyptal insulating lacquer.

You can probably buy it at the electrical supply store, but the web site
has neat history and a list of products.

Note: I do not work for them or have any financial interest, but I do use
their insulating lacquer.

Oh - and it is very low vapor pressure when dry. Would not use it on a
microscope vacuum system, but it used to be known as "the young
metallurgist's friend" because when you simply could not find the leak in
your vacuum furnace, the trick was to paint all soldered joints with
glyptal while under vacuum; no more leak.

Regards,
Andrew


At 08:33 AM 3/6/2007, you wrote:



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From: kenconverse-at-qualityimages.biz
Date: Tue, 6 Mar 2007 10:23:18 -0600
Subject: [Microscopy] viaWWW: Electrically Insulating Lacqer or Paint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Mark,
Like the others, since we're lacking some details, I can't promise that this
is your solution, but there are specialty spray acrylics available from the
likes of Newark Electronics that insulate at about 1kV/mil. I've had good
luck with Krylon clear acrylic spray paint. I don't think it's
substantially different (although we have some paint folks on the
listserver who can correct me if I'm wrong) and it's cheap and easy to find.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz



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Title-Subject: [Filtered] Electrically Insulating Lacqer or Paint

Question: I am looking for an electrically-insulating lacquer or paint that
does not need to be baked or heated in order to adhere to a metal surface.

---------------------------------------------------------------------------

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==============================Original Headers==============================
24, 30 -- From kenconverse-at-qualityimages.biz Tue Mar 6 10:23:18 2007
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From: mager-at-interchange.ubc.ca
Date: Tue, 6 Mar 2007 12:02:36 -0600
Subject: [Microscopy] viaWWW: Electrically Insulating Lacqer or Paint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Mark,
The classic for this purpose is stopping lacquer, available from suppliers
of electro-polishing and polishing equipment. We get ours from Tolber. It is
a red lacquer, thinned and/or removed with acetone, that is painted on to
mask off parts that are not to be electro-polished.
Good luck,

Mary Mager
Electron Microscopist
Materials Eng. UBC
#309 - 6350 Stores Road
Vancouver, B.C. V6T 1Z4
Canada
Tel: 604-822-5648
Fax: 604-822-3619
email: mager-at-interchange.ubc.ca

-----Original Message-----
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Title-Subject: [Filtered] Electrically Insulating Lacqer or Paint

Question: I am looking for an electrically-insulating lacquer or paint that
does not need to be baked or heated in order to adhere to a metal surface.

---------------------------------------------------------------------------

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From: twigg-at-estd.nrl.navy.mil
Date: Tue, 6 Mar 2007 12:54:21 -0600
Subject: [Microscopy] viaWWW: More 0n - Electrically Insulating Lacquer or Paint

Contents Retrieved from Microscopy Listserver Archives
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Email: twigg-at-estd.nrl.navy.mil
Name: Mark Twigg

Organization: NRL

Title-Subject: [Filtered] Electrically Insulating Lacquer or Paint

Question: I agree with the inquiries that I should have provided a more detailed description of my needs. The lacquer or paint that I need should have the following attributes:

1. Electrically isulating, but it only needs to stand up to 20 V.

2. Low vapor pressure for high vacuum use--it will coat a small region (~3 mm x 3mm) of a transmission electron microscope sample holder.

3. Must go on without heat or baking--I don't want to bake my sample holder.

4. It will not need to withstand high temperatures. It will be used at room temperature.

Thanks again,

Mark

---------------------------------------------------------------------------

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From: kenconverse-at-qualityimages.biz
Date: Tue, 6 Mar 2007 14:17:29 -0600
Subject: [Microscopy] viaWWW: More 0n - Electrically Insulating Lacquer or

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Mark,
I'd be inclined to go with the clear acrylic spray paint. You can keep the
layer extremely thin to avoid any serious outgassing. I'm assuming that the
beam is not going to hit this area, since you said the temperature would be
room temperature. The other items mentioned would probably also work well,
although you might have to deal with a considerably thicker layer and,
therefore, more significant outgassing initially.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz



-----Original Message-----
X-from: twigg-at-estd.nrl.navy.mil [mailto:twigg-at-estd.nrl.navy.mil]
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To: kenconverse-at-qualityimages.biz

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Email: twigg-at-estd.nrl.navy.mil
Name: Mark Twigg

Organization: NRL

Title-Subject: [Filtered] Electrically Insulating Lacquer or Paint

Question: I agree with the inquiries that I should have provided a more
detailed description of my needs. The lacquer or paint that I need should
have the following attributes:

1. Electrically isulating, but it only needs to stand up to 20 V.

2. Low vapor pressure for high vacuum use--it will coat a small region (~3
mm x 3mm) of a transmission electron microscope sample holder.

3. Must go on without heat or baking--I don't want to bake my sample
holder.

4. It will not need to withstand high temperatures. It will be used at
room temperature.

Thanks again,

Mark

---------------------------------------------------------------------------

==============================Original Headers==============================
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30, 30 -- From kenconverse-at-qualityimages.biz Tue Mar 6 14:17:29 2007
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From: dac-at-research.umass.edu
Date: Tue, 6 Mar 2007 14:48:14 -0600
Subject: [Microscopy] viaWWW: More 0n - Electrically Insulating Lacquer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Mark,

Since it is a low voltage insulation, what about just a dab of formvar
in chloroform or 1,2-dichlorethane? Make it a bit thick (2% - 5% ?) so
it doesn't flow too much. Formvar is the insulation once (still?) used
on "magnet wire" - they probably use more advanced things for hi-temp
purposes these days. But it should give reasonable adhesion to metal (as
in the wire usage), should dry/outgas at RT and be OK in a high vacuum
(as in thin support films). And most EM labs have some. In a pinch,
polystyrene dissolved in CHCl3 will do the same - people use it for
films (here you could use a bit of dispo petri dish...).

Dale


kenconverse-at-qualityimages.biz wrote:
} ----------------------------------------------------------------------------
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}
} Mark,
} I'd be inclined to go with the clear acrylic spray paint. You can keep the
} layer extremely thin to avoid any serious outgassing. I'm assuming that the
} beam is not going to hit this area, since you said the temperature would be
} room temperature. The other items mentioned would probably also work well,
} although you might have to deal with a considerably thicker layer and,
} therefore, more significant outgassing initially.
}
} Ken Converse
} owner
}
} QUALITY IMAGES
} Servicing Scanning Electron Microscopes
} Since 1981
} 474 So. Bridgton Rd.
} Bridgton, ME 04009
} 207-647-4348
} Fax 207-647-2688
} kenconverse-at-qualityimages.biz
} qualityimages.biz
}
}
}
} -----Original Message-----
} X-from: twigg-at-estd.nrl.navy.mil [mailto:twigg-at-estd.nrl.navy.mil]
} Sent: Tuesday, March 06, 2007 1:57 PM
} To: kenconverse-at-qualityimages.biz
} Subject: [Microscopy] viaWWW: More 0n - Electrically Insulating Lacquer or
} Paint
}
}
}
}
}
} ----------------------------------------------------------------------------
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} Email: twigg-at-estd.nrl.navy.mil
} Name: Mark Twigg
}
} Organization: NRL
}
} Title-Subject: [Filtered] Electrically Insulating Lacquer or Paint
}
} Question: I agree with the inquiries that I should have provided a more
} detailed description of my needs. The lacquer or paint that I need should
} have the following attributes:
}
} 1. Electrically isulating, but it only needs to stand up to 20 V.
}
} 2. Low vapor pressure for high vacuum use--it will coat a small region (~3
} mm x 3mm) of a transmission electron microscope sample holder.
}
} 3. Must go on without heat or baking--I don't want to bake my sample
} holder.
}
} 4. It will not need to withstand high temperatures. It will be used at
} room temperature.
}
} Thanks again,
}
} Mark
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
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} 30, 30 -- From kenconverse-at-qualityimages.biz Tue Mar 6 14:17:29 2007
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5, 22 -- From dac-at-research.umass.edu Tue Mar 6 14:48:14 2007
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From: gcouger-at-science-info.net
Date: Tue, 6 Mar 2007 15:11:03 -0600
Subject: [Microscopy] Re: viaWWW: Electrically Insulating Lacqer or Paint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

McMaster Carr www.mcmaster.com has 3 in spray cans 7437K17 7437K16
7437K18 and if it doesn't need to flex the age old solution of Shellac
should work as well.

Gordon
Gordon Couger
Stillwater OK
www.science-info.net

twigg-at-estd.nrl.navy.mil wrote:
} Email: twigg-at-estd.nrl.navy.mil
} Name: Mark Twigg
}
} Organization: NRL
}
} Title-Subject: [Filtered] Electrically Insulating Lacqer or Paint
}
} Question: I am looking for an electrically-insulating lacquer or paint that does not need to be baked or heated in order to adhere to a metal surface.
}
} -


==============================Original Headers==============================
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From: garygill-at-dcla.com
Date: Wed, 7 Mar 2007 09:22:31 -0600
Subject: [Microscopy] LM: camera adaptor for photomicrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Can anyone recommend an inexpensive adaptor that will allow me to couple my
Fuji FinePix E550 digital camera to the trinocular port of my Olympus BX40?

Thanks.

Gary Gill



==============================Original Headers==============================
2, 17 -- From garygill-at-dcla.com Wed Mar 7 09:22:30 2007
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2, 17 -- From: Gary Gill {garygill-at-dcla.com}
2, 17 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-Microscopy.Com}
2, 17 -- Subject: LM: camera adaptor for photomicrography
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From: NWWhite-at-bwxt.com
Date: Wed, 7 Mar 2007 09:58:38 -0600
Subject: [Microscopy] LM: camera adaptor for photomicrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I have not used them, nor have any interest, but you might check with:
www.scopetronix.com

Woody White
BWXT Services


-----Original Message-----
X-from: garygill-at-dcla.com [mailto:garygill-at-dcla.com]
Sent: Wednesday, March 07, 2007 10:23 AM
To: White, Woody N.

Can anyone recommend an inexpensive adaptor that will allow me to couple my
Fuji FinePix E550 digital camera to the trinocular port of my Olympus BX40?

Thanks.

Gary Gill



==============================Original Headers==============================
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==============================Original Headers==============================
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From: microscopytoday-at-tampabay.rr.com
Date: Wed, 7 Mar 2007 10:26:29 -0600
Subject: [Microscopy] LM: camera adaptor for photomicrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Scopetronix apparently doesn't exist anymore. Their address is an empty
building, no one answers their phone. If anyone turns them up, please
let me know.

Ron Anderson, Editor
Microscopy Today

NWWhite-at-bwxt.com wrote:
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I have not used them, nor have any interest, but you might check with:
} www.scopetronix.com
}
} Woody White
} BWXT Services
}
}
} -----Original Message-----
} X-from: garygill-at-dcla.com [mailto:garygill-at-dcla.com]
} Sent: Wednesday, March 07, 2007 10:23 AM
} To: White, Woody N.
} Subject: [Microscopy] LM: camera adaptor for photomicrography
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Can anyone recommend an inexpensive adaptor that will allow me to couple my
} Fuji FinePix E550 digital camera to the trinocular port of my Olympus BX40?
}
} Thanks.
}
} Gary Gill
}
}
}
} ==============================Original Headers==============================
} 2, 17 -- From garygill-at-dcla.com Wed Mar 7 09:22:30 2007
} 2, 17 -- Received: from sturgeon.dcla.com (dcla.com [207.67.84.53] (may be forged))
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} 2, 17 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-Microscopy.Com}
} 2, 17 -- Subject: LM: camera adaptor for photomicrography
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From: mike.reedy-at-cellbio.duke.edu
Date: Wed, 7 Mar 2007 11:21:47 -0600
Subject: [Microscopy] Re: LM: camera adaptor for photomicrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Look into both Scopetronix and the Microscope Store CamAdapter kits.

Latter is at www.microscope-store.com/index.php/cPath/12_15

We have both kits available, but haven't had time
to evaluate carefully side-by-side. I think the
latter may include some features and a range of
specific add-on adapters that cover more ground;
add-on kit for your Fuji is listed at
www.microscope-store.com/index.php/cPath/12_19

I recommend you talk to both companies by phone and take notes.
-mike reedy-



At 9:27 AM -0600 3/7/07, garygill-at-dcla.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
Dept. Cell Biology, Box 3011 (for U.S. Mail)
458 Alex Sands Bldg, Research Dr (courier)
Durham, NC 27710

Office 919-668-2534
Lab 919-684-5674
Fax 919-681-9929
mike.reedy-at-cellbio.duke.edu
http://note.cellbio.duke.edu/Faculty/Research/Reedy.html


==============================Original Headers==============================
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12, 18 -- From: Mike Reedy {mike.reedy-at-cellbio.duke.edu}
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From: smithj-at-exchange.winthrop.edu
Date: Wed, 7 Mar 2007 11:26:06 -0600
Subject: [Microscopy] RE: LM: camera adaptor for photomicrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Try Bobby Martin at Martin Microscope (www.martinmicroscope.com). I
don't know about the FinePix, but they make and sell an adapter for
the Canon digital cameras.
Disclaimer: No commercial connection except that of a satisfied customer.
Julian

} } Can anyone recommend an inexpensive adaptor that will allow me to couple my
} } Fuji FinePix E550 digital camera to the trinocular port of my Olympus BX40?
} }
} } Thanks.
} }
} } Gary Gill
} }

--
Julian P.S. Smith III
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC 29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)

==============================Original Headers==============================
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From: garygill-at-dcla.com
Date: Wed, 7 Mar 2007 11:32:14 -0600
Subject: Re: [Microscopy] LM: camera adaptor for photomicrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Someone at The Microscope Store is emailing me information. Total cost is
about $300. Thanks for the leads, Mike and everyone.

Gary Gill

-----Original Message-----
X-from: Mike Reedy [mailto:mike.reedy-at-cellbio.duke.edu]
Sent: Wednesday, March 07, 2007 12:22 PM
To: garygill-at-dcla.com
Cc: Microscopy Listserver


--
-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
Dept. Cell Biology, Box 3011 (for U.S. Mail)
458 Alex Sands Bldg, Research Dr (courier)
Durham, NC 27710

Office 919-668-2534
Lab 919-684-5674
Fax 919-681-9929
mike.reedy-at-cellbio.duke.edu
http://note.cellbio.duke.edu/Faculty/Research/Reedy.html


==============================Original Headers==============================
15, 20 -- From garygill-at-dcla.com Wed Mar 7 11:32:14 2007
15, 20 -- Received: from sturgeon.dcla.com (dcla.com [207.67.84.53] (may be forged))
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15, 20 -- From: Gary Gill {garygill-at-dcla.com}
15, 20 -- To: "'Mike Reedy'" {mike.reedy-at-cellbio.duke.edu} ,
15, 20 -- Gary Gill
15, 20 -- {garygill-at-dcla.com}
15, 20 -- Cc: Microscopy Listserver {microscopy-at-microscopy.com}
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From: PWebster-at-hei.org
Date: Wed, 7 Mar 2007 11:37:08 -0600
Subject: [Microscopy] EM & LM Digital database

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

We need a way to catalog stored images (EM & LM) together with experimental
data (text) for access by in-house researchers as well as off-site users.

Does anyone know of any commercial products available that would fit our
needs?

We have someone here who would like to try to develop this but it will be a
waste of time and money if something is already available.

Regards,

Paul.


Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
(213) 273 8026
pwebster-at-hei.org



==============================Original Headers==============================
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10, 18 -- User-Agent: Microsoft-Entourage/11.3.3.061214
10, 18 -- Date: Wed, 07 Mar 2007 09:37:06 -0800
10, 18 -- Subject: EM & LM Digital database
10, 18 -- From: "Webster, Paul" {PWebster-at-hei.org}
10, 18 -- To: {Microscopy-at-Microscopy.Com}
10, 18 -- Message-ID: {C2143842.FBF9%PWebster-at-hei.org}
10, 18 -- Thread-Topic: EM & LM Digital database
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From: edelmare-at-muohio.edu
Date: Wed, 7 Mar 2007 12:11:44 -0600
Subject: [Microscopy] EM Field sources

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

O.k., I'm looking for some help in understanding electro-magnetic
fields, and the sources generating them.

Here's the issue in my lab we've gone from 0.46mG to 8.7mG in X-axis
EMF-AC, and 1.1mG to 15.2mG in Z-axis EMF-AC. These obviously push
beyond specs for the SEM in the room.

So I am looking for an education. I have no idea of the context to
put this in. What would or could cause these kinds of EM Field
increases (15 to 20 times)? A single new 110V outlet? A new 1000W
UPS in a near by room? A 480V feeder line? A new HVAC blower motor?

These were measurements were made in the middle of a 10ft x 10ft x
9ft room (3.2m x 3.2m x 3m) Next to an SEM, in the Off state (no
power). I operate the rooms on two sides and below, and I have
checked the labs on the other lateral sides and the floor above - no
new equipment or significant electrical services within at least 15
to 50 feet in these spaces. BUT "signifcant" what is significant?
Am I looking for a new 110v outlet? or a computer UPS? Or am I
looking for something in the next building? Or 150feet down the
hall? I have not dug through the ceilings yet to look for any
"hidden" changes but again I do not know what I should be looking
for.

Am I looking for an induced current coming through an eithernet
cable?

I do not have a hand held gauss-meter to track things down. Do I
need one?

Any help would be great. Thanks folks.


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"A main-frame: The biggest PC peripheral available."


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From: tivol-at-caltech.edu
Date: Wed, 7 Mar 2007 12:38:41 -0600
Subject: [Microscopy] Re: EM & LM Digital database

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 7, 2007, at 9:37 AM, PWebster-at-hei.org wrote:

} We need a way to catalog stored images (EM & LM) together with
} experimental
} data (text) for access by in-house researchers as well as off-site
} users.
}
} Does anyone know of any commercial products available that would fit
} our
} needs?
}
} We have someone here who would like to try to develop this but it will
} be a
} waste of time and money if something is already available.
}
Dear Paul,
Are you talking about film or digital images? If the latter, Leginon
not only collects data automatically from a selection of targets, but
it also archives all the data in a database--I think that feature alone
is worth using Leginon for. The program is free, although someone from
your lab will have to attend a training class (also free). Leginon
requires pretty large computer resources, but if you are archiving a
lot of images you will need that in any case. I am not connected with
the purveyors (Clint Potter & Bridget Carrigher) of Leginon except as a
satisfied user, but, in the interest of full disclosure, Christian
Suloway, the developer of many of the modules in Leginon, is a graduate
student here, so we do have better access to fixing bugs than the
average installation.
Yours,
Bill Tivol, PhD
EM Scientist
Electron Cryo-Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


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From: martini-at-accurel.com
Date: Wed, 7 Mar 2007 16:38:30 -0600
Subject: [Microscopy] viaWWW: Gatan duomill spares - Available

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Email: martini-at-accurel.com
Name: Martin Izquierdo

Organization: Accurel

Title-Subject: [Filtered] Gatan duomill spares

Question: After rummaging through some cabinets I found some Gatan Duomill spares. I have some o-rings, cathodes, even a autoterminator (and other misc duomill related things).

All the items are "free", but you do have to pay for the shipping.

First person who emails me get it.

Regards,

Martin

---------------------------------------------------------------------------

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10, 12 -- Subject: viaWWW: Gatan duomill spares - Available
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From: greta.rennings-at-web.de
Date: Wed, 7 Mar 2007 16:40:40 -0600
Subject: [Microscopy] AskAMicroscopist: analysis of paper

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (greta.rennings-at-web.de) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, March 7, 2007 at 13:41:04
---------------------------------------------------------------------------

Email: greta.rennings-at-web.de
Name: Greta Rennings

Organization: KU Leuven

Education: Graduate College

Location: Leuven, Brabant, Belgium

Question: Dear ladies and gentlemen,

do you have an idea which microscopic technique would be suitable for analysis of paper?
Firstly for 3D I did trials with CLSM, which were not truly successful as grey scale differences were too low. Other techniques I know would require splitting or sectioning, as far as I know- do you have further experience?
Secondly for 2D I tried light microscopy and SEM, but grey scale differences were to low here too in order to differentiate between components. Could TEM be useful? What could help to get better distinguishable features?

Thank you very much in advance for your support!
Kind regards,
Greta

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From: paul.gerroir-at-xrcc.xeroxlabs.com
Date: Thu, 8 Mar 2007 07:38:36 -0600
Subject: [Microscopy] Darkroom Equipment - Surplus Enlargers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Good morning,

To those of you who have gone digital and may have an enlarger
collecting dust I might like to hear from you. I have rekindled an
interest in B&W photography and am in need of a used enlarger. Because
shipping will be a major expense I would initially like to limit my
search to the southern Ontario/ upper New York state areas. If you have
an enlarger that you have been considering for disposal please contact
me.



Thanks,

Paul


Paul J. Gerroir

Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: 905-823-7091, ext.216
FAX: 905-822-7022
e-mail: paul.gerroir-at-xrcc.xeroxlabs.com




==============================Original Headers==============================
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From: jcoleman-at-rdg.boehringer-ingelheim.com
Date: Thu, 8 Mar 2007 07:49:04 -0600
Subject: [Microscopy] EM Field sources

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Jim


-----Original Message-----
X-from: Jones,Dr.,Paul-James AN BIP-US-R
Sent: Thursday, March 08, 2007 7:26 AM
To: Coleman,Dr.,James AN BIP-US-R

O.k., I'm looking for some help in understanding electro-magnetic
fields, and the sources generating them.

Here's the issue in my lab we've gone from 0.46mG to 8.7mG in X-axis
EMF-AC, and 1.1mG to 15.2mG in Z-axis EMF-AC. These obviously push
beyond specs for the SEM in the room.

So I am looking for an education. I have no idea of the context to
put this in. What would or could cause these kinds of EM Field
increases (15 to 20 times)? A single new 110V outlet? A new 1000W
UPS in a near by room? A 480V feeder line? A new HVAC blower motor?

These were measurements were made in the middle of a 10ft x 10ft x
9ft room (3.2m x 3.2m x 3m) Next to an SEM, in the Off state (no
power). I operate the rooms on two sides and below, and I have
checked the labs on the other lateral sides and the floor above - no
new equipment or significant electrical services within at least 15
to 50 feet in these spaces. BUT "signifcant" what is significant?
Am I looking for a new 110v outlet? or a computer UPS? Or am I
looking for something in the next building? Or 150feet down the
hall? I have not dug through the ceilings yet to look for any
"hidden" changes but again I do not know what I should be looking
for.

Am I looking for an induced current coming through an eithernet
cable?

I do not have a hand held gauss-meter to track things down. Do I
need one?

Any help would be great. Thanks folks.


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"A main-frame: The biggest PC peripheral available."


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From: martini-at-accurel.com
Date: Thu, 8 Mar 2007 07:59:19 -0600
Subject: [Microscopy] viaWWW: Gatan duomill spares - Gone

Contents Retrieved from Microscopy Listserver Archives
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Email: martini-at-accurel.com
Name: Martin Izquierdo

Organization: Accurel

Title-Subject: [Filtered] Gatan duomill spares - Gone

Question: Wow, I received plenty of responses regarding the Duomill spares and I do have a winner.

Regards,

Martin

---------------------------------------------------------------------------

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From: colijn.1-at-osu.edu
Date: Thu, 8 Mar 2007 08:22:48 -0600
Subject: [Microscopy] Re: EM Field sources

Contents Retrieved from Microscopy Listserver Archives
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Richard,

We've had to fight field issues in an old building. We've found that
most of our fields can be accounted for by someone making a
ground-neutral bond, i.e. tying a neutral to a ground line. This,
while it is low voltage, can generate large currents and hence large
fields. Neutral-ground bonds are against the electrical code but
they still occur.

I can recommend a hand-held gaussmeter which is good to 0.01mG. We
got ours from
http://www.lessemf.com/combi.html. Scroll down to the model
A480. $169 (just a satisfied customer)

It is a single axis meter which we have found to be more useful in
tracking down stray fields than a tri-axial unit. It has been
indispensable, particularly at the price.

Good luck,
Henk

At 01:13 PM 03/07/07, edelmare-at-muohio.edu wrote:



} ----------------------------------------------------------------------------
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Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


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From: ceren_b68-at-hotmail.com
Date: Thu, 8 Mar 2007 08:28:26 -0600
Subject: [Microscopy] viaWWW: Lectin for FtsZ protein

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Email: ceren_b68-at-hotmail.com
Name: Ceren Bykkayalar

Organization: Gebze Institute of Technology

Title-Subject: [Filtered] Lectin for FtsZ protein

Question: Hi, everyone
I am a M.S student and I would like to study the bacterial cytoskeletal elements for various bacteria (E. coli for the moment). I would like to label FtsZ, MreB or such proteins with lectins and then label them with gold or directly label these proteins with gold-labelled lectins and then observe the results in TEM.

Does anyone know any specific lectins for bacterial cytoskeletal proteins or anything about this subject?

Any help is appreciated
Thank you in advance


Ceren Buyukkayalar
M.S student
Gebze Institute of Technology
Turkey

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From: john.mardinly-at-intel.com
Date: Thu, 8 Mar 2007 10:51:47 -0600
Subject: [Microscopy] EM Field sources

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Richard;
A cheap ( {$100), readily available handheld field meter like the
Extech 480823, as recommended by Professor David Muller, will help you
locate the source of the fields.
http://www.extech.com/instrument/products/451_499/480823.html


John Mardinly
Intel Corporation

This comment does not represent an opinion of Intel Corporation.

-----Original Message-----
X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu]
Sent: Wednesday, March 07, 2007 10:12 AM
To: Mardinly, John

O.k., I'm looking for some help in understanding
electro-magnetic
fields, and the sources generating them.

Here's the issue in my lab we've gone from 0.46mG to 8.7mG in
X-axis
EMF-AC, and 1.1mG to 15.2mG in Z-axis EMF-AC. These obviously push
beyond specs for the SEM in the room.

So I am looking for an education. I have no idea of the context
to
put this in. What would or could cause these kinds of EM Field
increases (15 to 20 times)? A single new 110V outlet? A new 1000W
UPS in a near by room? A 480V feeder line? A new HVAC blower motor?

These were measurements were made in the middle of a 10ft x 10ft
x
9ft room (3.2m x 3.2m x 3m) Next to an SEM, in the Off state (no
power). I operate the rooms on two sides and below, and I have
checked the labs on the other lateral sides and the floor above - no
new equipment or significant electrical services within at least 15
to 50 feet in these spaces. BUT "signifcant" what is significant?
Am I looking for a new 110v outlet? or a computer UPS? Or am I
looking for something in the next building? Or 150feet down the
hall? I have not dug through the ceilings yet to look for any
"hidden" changes but again I do not know what I should be looking
for.

Am I looking for an induced current coming through an eithernet
cable?

I do not have a hand held gauss-meter to track things down. Do
I
need one?

Any help would be great. Thanks folks.


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"A main-frame: The biggest PC peripheral available."


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From: rothbardd-at-netscape.net
Date: Thu, 8 Mar 2007 11:52:01 -0600
Subject: [Microscopy] AskAMicroscopist: analysis of paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

If you what you mean by "analysis of paper" is a full 3D structure, the
best I have ever seen was done by a company offering a proprietary
service where serial sections are imaged on the blockface. I have no
commercial interest and am not endorsing them in any way.

http://www.microsciencegroup.com/applications_publications.htm
This page links to their applications papers. Select the one called
"Filtration + Separation" for a Sept 2001 publication showing an
example of filter paper. [I was surprised to see they also had a link
to a Newsweek story in which I was interviewed.]

I, and many others, have had various degrees of success cutting and
registering serial microtome sections of embedded paper. My preferred
current method is SEM imaging after cross sections are prepared by
embedding, polishing, and etching. It is illustrated in my abstract in
the 2002 MSA Annual Meeting, Page 178. A key reference for the method is
G.J. Williams and J.G. Drummond, J. Pulp and Paper Science, V26 (2000),
P. 188

Final note. Surface structure is very well characterized by some of the
modern white light interferometers. No preparation needed.

David R. Rothbard, Ph.D.
BEP

-----Original Message-----
X-from: greta.rennings-at-web.de
To: rothbardd-at-netscape.net
Sent: Wed, 7 Mar 2007 5:40 PM

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by
(greta.rennings-at-web.de) from
http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Wednesday, March 7, 2007 at 13:41:04
-------------------------------------------------------------------------
--

Email: greta.rennings-at-web.de
Name: Greta Rennings

Organization: KU Leuven

Education: Graduate College

Location: Leuven, Brabant, Belgium

Question: Dear ladies and gentlemen,

do you have an idea which microscopic technique would be suitable for
analysis
of paper?
Firstly for 3D I did trials with CLSM, which were not truly successful
as grey
scale differences were too low. Other techniques I know would require
splitting
or sectioning, as far as I know- do you have further experience?
Secondly for 2D I tried light microscopy and SEM, but grey scale
differences
were to low here too in order to differentiate between components.
Could TEM be
useful? What could help to get better distinguishable features?

Thank you very much in advance for your support!
Kind regards,
Greta

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From: bozzola-at-siu.edu
Date: Thu, 8 Mar 2007 14:05:51 -0600
Subject: [Microscopy] CMOS versus CCD cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

What are the advantages/disadvantages of CMOS compared to CCD cameras?

Are they comparable in terms of sensitivity, resolution and durability?

Thank you.

JB
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
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From: Mike.Bode-at-olympus-sis.com
Date: Thu, 8 Mar 2007 14:46:20 -0600
Subject: [Microscopy] CMOS versus CCD cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Both CMOS and CCD sensors convert light into electrical signals that
can be understood and interpreted by a computer, and they both use the
same physical effects for the conversion, so the underlying physical
limitations are the same. The difference is the technology used:

In a CCD chip, all the collected information in the many pixels of the
chip are processed by a very limited number of electronic elements
(voltage amplifier, etc.), often only one. While that can be a drawback
in terms of speed, it does help with the uniformity of the signal. Plus,
the individual pixels of the chip can be almost entirely used for
converting photons to electrons, i.e., the CCD chips use almost 100% of
the available area as light sensitive areas. For even higher sensitivity
one can thin down the chips and illuminate them from the back side.

A CMOS (Complementary Metal-Oxide Semiconductor) chip uses a different
technology for the fabrication. It is actually the same process that is
used for regular electronics, so it is easy to also integrate some
electronic devices on the chip. In a CMOS chip, each pixel has its own
voltage amplifier and perhaps other electronics. In comparison to a CCD
chip then, the uniformity is usually worse (many amplifiers as opposed
to one), and the sensitivity is not as good (some "dead area" for each
pixel). On the other hand, CMOS chips can integrate other electronics on
the same chip ("camera on a chip"), and they can be much more energy
efficient (hence their use in consumer type cameras that depend on
batteries). The fact that the electronic circuits on each pixel eat up
some space made CMOS quite useless for light sensitive chips until a few
years ago, when the dimensions of those electronic circuits had shrunk
to a size that allowed a significant area of each pixel to be used as
light sensitive area.

Factors like resolution are not quite as simple as they appear. You can
make cameras with really small pixels, but what happens is that the
number of electrons you can store in each pixel becomes smaller and
smaller. Comparing this number to the spontaneous generation of
electrons (noise), you can see that the dynamic range of the camera gets
reduced. For example, if you can store 100,000 electrons, and your noise
level is 50 electrons, you can distinguish 2000 intensity levels, or
about 11 bits. If your storage size shrinks to 10,000 electrons, you end
up with 200 levels, or less than 8 bit. On the other hand, if you
increase the pixel size, it takes longer to read them out, and the chip
gets a lot bigger (=$$). But this applies to both CMOS and CCD. So, for
any given set of parameters there is a different chip that is best. That
could be a CMOS or CCD chip.

My personal opinion is that we will see more development in the CMOS
sector (due to their use in consumer cameras), and they will start to
eat into the CCD market. But I don't think that the CCD market will
totally go away, especially in the scientific arena, where it is often
necessary to squeeze some signal out of the last photon.

As far as durability goes, I don't see a reason why a CMOS chip should
have a durability that is different from a CCD chip.

If you need more information, just google "CMOS CCD", and you'll get a
lot of information that goes deeper than what I wrote up here.



Michael Bode, Ph.D.

General Manager

OLYMPUS SOFT IMAGING SOLUTIONS
12596 West Bayaud Ave #300
Lakewood, CO 80228
USA
Tel.: +1 (303) 234-9270
Fax.: +1 (303) 234-9271
E-mail: Mike.Bode-at-olympus-sis.com
www.olympus-sis.com

-----Original Message-----
X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
Sent: Thursday, March 08, 2007 13:11
To: Mike Bode

What are the advantages/disadvantages of CMOS compared to CCD cameras?

Are they comparable in terms of sensitivity, resolution and durability?

Thank you.

JB
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750
Communications Drive - MC 4402 Southern Illinois University Carbondale,
IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
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From: PWebster-at-hei.org
Date: Thu, 8 Mar 2007 14:52:18 -0600
Subject: [Microscopy] RE; EM & LM Digital database

Contents Retrieved from Microscopy Listserver Archives
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Hello again,

Many thanks for the replies concerning digital imaging software. I received
many replies, and also many requests to share the information I collected.

I also met up with a few people I have not contacted in a while and caught
up on a bit of gossip. To all - please stay in contact.

Below is a condensed summary of the replies I received. I have removed
identifiers so that contributors can remain anonymous.

I would say that Hitachi and Olympus (SIS) have been very good at supplying
detail of the software they produce, and I thank them for that. I would
suggest that anyone with specific interests in those software packages
contact thir local people. I can also provide contact info if needed.

There is much more available than I imagined so the message is long.

Many thanks to eveyone who took the time to reply, and best wishes to you
all.

Regards,

Paul

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
(213) 273 8026
pwebster-at-hei.org


Summary:
The quick answer is a database (ie Filemaker Pro Server, etc) but for a
valadation product BioImagene Scientific Image Management System has a
good product - BUT it costs.
__________________________________________________________________________
Are you talking about film or digital images? If the latter, Leginon
not only collects data automatically from a selection of targets, but
it also archives all the data in a database--I think that feature alone
is worth using Leginon for. The program is free, although someone from
your lab will have to attend a training class (also free). Leginon
requires pretty large computer resources, but if you are archiving a
lot of images you will need that in any case. I am not connected with
the purveyors (Clint Potter & Bridget Carrigher) of Leginon except as a
satisfied user.
__________________________________________________________________________
I think Quartz PCI software, http://www.qrtz.com/network.html ,may be
just what you are looking for.
__________________________________________________________________________
Extensis Portfolio 8 is one Strong system. I know several of our
Histology / Pathology EM users have a hospital wide system that is very
secure and very stable. The system is available for individual users,
workgroups and as Client/Server solutions (but big $$ for the Bigger
systems).
__________________________________________________________________________
Extensis Portfolio 8 is one Strong system. I know several of our
Histology / Pathology EM users have a hospital wide system that is very
secure and very stable. The system is available for individual users,
workgroups and as Client/Server solutions (but big $$ for the Bigger
systems).
__________________________________________________________________________
Aperio: http://www.aperio.com/productsservices/prod-spectrum.asp
__________________________________________________________________________
Media Cybernetics' IQBase:
http://www.mediacy.com/index.aspx?page=IQBase
__________________________________________________________________________
Have you considered OME at http://www.openmicroscopy.org/
__________________________________________________________________________
I would contact:
OLYMPUS SOFT IMAGING SOLUTIONS
12596 West Bayaud Ave #300
Lakewood, CO 80228
USA
Tel.: +1 (303) 234-9270
Fax.: +1 (303) 234-9271
E-mail: Mike.Bode-at-olympus-sis.com
Or Jason Wickersham {Jason.Wickersham-at-olympus-sis.com}
www.olympus-sis.com
__________________________________________________________________________
see http://www.cerious.com {http://www.cerious.com/} . ThumbsPlus should
do what you want - if not you may need to go to something like Image Central
- http://www.AIC-ImageCentral.com
__________________________________________________________________________

You're after "digital asset management software," yes, DAM for short
You can google the term to get a bunch of vendors. You really don't need
to have your IT department develop the software for you. In addition,
most vendors will let you try out their product for 2 weeks or so before
you buy - I strongly recommend simultaneously trying out several so that
you can compare them.

CanvasX may be what you need. The price is certainly right (~$600). I
don't have experience with it, but it's aimed at a technical work group
market, people doing technical drawings, that sort of thing, from what I
see, it looks like the images can be extensively annotated. You want to
see how many sites can upload images; I suspect it's only one. It's OK
if it's all within one lab, but difficult if several labs want to load
images to the same site. If you're considering putting up videos in
addition to still images, you want to make sure it can handle them.
Check it out at: http://www.acdamerica.com/

Image folio looks like it's aimed at a commercial market (ie, companies
selling images), but it may suit your needs, with the same things to
look for as with CanvasX - I wasn't convinced that this package offers
much in the way of annotation capability (there are 3 packages, from $89
to $750): http://imagefolio.com/

We're using Contentdm (see our site http://cellimages.ascb.org/) -
$10,000 initial license for up to 10,000 images, annual maintenance of ~
$2,000, with lots of tools and considerable flexibility, you can have
password controlled access, intended for display over the Internet:
http://www.dimema.com/ If you have this much $$$, you could create
collections for every user. The license comes with 50 "Acquisitions
Stations," which I believe means that you can have 50 computers with the
capability to upload images. Take a look at our site, we've configured
it to handle video as well as still images. You can also put together
what they call "compound objects" - it's a way of grouping related items
(eg, all the images from a given experiment) so that they all come up
together. We can certainly help you out if you decide to go this
route.

This is a nice grouping of a number of other DAM systems:
http://web.reed.edu/digital_asset_mgmt/systems.html


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From: gary-at-gaugler.com
Date: Thu, 8 Mar 2007 14:59:08 -0600
Subject: [Microscopy] Re: CMOS versus CCD cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

As usual, it depends.

Here is a link to a good comparison:

http://www.dalsa.com/markets/ccd_vs_cmos.asp

The uniformity of CCD imaging is a big deal,
as well as lower noise (which the link does not
really address).

This link:

http://www.imaging-resource.com/PRODS/D30/D30A4.HTM

does discuss noise. Both links discuss the added
external circuitry needed to read out the analog
data from CCD. But having on-chip A/D increases
chip size and also puts pressure on being able to
reduce pixel dimensions. The recent availability
of smaller feature size CMOS helps in this respect.
It also helps with off-chip signal processing which
both sensors have to have.

The choice depends on application and target price.
For imaging satellites, CCD is the choice. So too
for high quality telescope cameras (Peltier cooled).
To my knowledge, no one has made a cooled CMOS sensor.
CMOS has inherently more shot and thermal noise than
CCD. This does not really affect the consumer market.
But for professional users, their cameras use CCD.
The use of CMOS by Canon in an SLR is a first and will be
interesting to follow.

Hope this helps.

gary g.



At 12:07 PM 3/8/2007, you wrote:




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From: camiller-at-anatomy.iupui.edu
Date: Thu, 8 Mar 2007 17:34:53 -0600
Subject: [Microscopy] viaWWW: abstract deadline for joint LAS meeting

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Email: camiller-at-anatomy.iupui.edu
Name: Caroline Miller

Organization: Indiana Microscopy Society

Title-Subject: [Filtered] reminder of abstract deadline for joint LAS meeting

Question: A reminder of abstract deadline for the Joint LAS spring meeting, "Imaging for Nanotechnology," being held in Indianapolis on April 20th and 21st. Deadline has been extended to March 15th.

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From: javaidqazi-at-kemet.com
Date: Thu, 8 Mar 2007 17:35:18 -0600
Subject: [Microscopy] viaWWW: OM Calibration standard Z direction

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Email: javaidqazi-at-kemet.com
Name: Javaid Qazi

Title-Subject: [Filtered] OM Calibration standard Z direction

Question: I am looking for a Z direction Calibration standard for an optical microscope which can do surface profiles, mag ranges from 20 to 1000x.

Please let me know offline what are my options.


Thanks

Javaid


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From: brayandtavsmom-at-comcast.net
Date: Thu, 8 Mar 2007 23:13:28 -0600
Subject: [Microscopy] viaWWW: EDS/WDS systems

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Email: brayandtavsmom-at-comcast.net
Name: Adena Rollins

Organization: Electron Microscopy Program (Delta College)

Title-Subject: [Filtered] EDS/WDS systems

Question: Hello all! I am doing a presentation on EDS and WDS systems and I can't seem to find any information on how much these systems would cost, ready for installation on any TEM/SEM. I understand that there are various types of detector systems..I would just like a ballpark estimate on what these systems might cost. Any information would be greatly appreciated. Thank you

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From: johnf-at-geology.wisc.edu
Date: Fri, 9 Mar 2007 09:31:20 -0600
Subject: [Microscopy] Carbon evaporation coaters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

A colleague recently told me that a carbon evaporation coater (for
sem/epma samples) now lists for over ~US$55K (full scale model-not
tabletop, with diffusion pump, no thickness monitor). This is
essentially the same unit that I purchased 13 years ago for ~$12K.

Does anyone have any idea why the price for such a unit has increased
by } 400% in 13 years? Are there still "simple full scale" (not
tabletop) models that sell at more "reasonable" prices? (He asked me
if I thought an NSF proposal reviewer would look askance as such a
high cost of a coater in a proposal...)

Thanks.

John
--
========================================================
John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480
Cameron Electron Microprobe Lab lab: (608) 265-4798
Dept of Geology & Geophysics fax: (608) 262-0693
University of Wisconsin home: (608) 274-2245
1215 West Dayton St. email: johnf-at-geology.wisc.edu
Madison, WI 53706 amateur radio: WA3BTA
Personal http://www.geology.wisc.edu/~johnf/
Probe lab http://www.geology.wisc.edu/~johnf/sx51.html
Probe Sign Up Calender:
http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi

"The first rule of all intelligent tinkering is to save every cog and
wheel." -- Aldo Leopold

"For a successful technology, reality must take precedence over
public relations, for Nature cannot be fooled." -- Richard P.
Feynman

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From: nairvinods-at-gmail.com
Date: Fri, 9 Mar 2007 12:06:46 -0600
Subject: [Microscopy] posting for a friend of a friend :)

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Dear Colleagues,

I am posting this on behalf of my friend.

Dr. Godfrey Mbah, faculty at a local college needs to use a Jumbo
Supercritical Point Dryer for his research. If anyone has one within
driving distance from Columbia, SC, please contact him directly. He is
willing to pay for use of the instrument. Godfrey can be reached at
Mbahg-at-benedict.edu.

Thanks for your help.

Soumitra

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From: tivol-at-caltech.edu
Date: Fri, 9 Mar 2007 13:12:58 -0600
Subject: [Microscopy] Re: Carbon evaporation coaters

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On Mar 9, 2007, at 7:31 AM, johnf-at-geology.wisc.edu wrote:

} A colleague recently told me that a carbon evaporation coater (for
} sem/epma samples) now lists for over ~US$55K (full scale model-not
} tabletop, with diffusion pump, no thickness monitor). This is
} essentially the same unit that I purchased 13 years ago for ~$12K.
}
} Does anyone have any idea why the price for such a unit has increased
} by } 400% in 13 years? Are there still "simple full scale" (not
} tabletop) models that sell at more "reasonable" prices? (He asked me
} if I thought an NSF proposal reviewer would look askance as such a
} high cost of a coater in a proposal...)
}
Hi John,
About 3 years ago I got a Cressington 208 evaporation system (both
carbon and the optional metal power supplies) from Pella, and have been
quite satisfied. The cost was about half your quote (but may have
increased). The web site is:

http://www.tedpella.com/cressing_html/intro.html

I have no relationship to Pella except as a satisfied user.
Yours,
Bill Tivol, PhD
EM Scientist
Electron Cryo-Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


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From: beaurega-at-westol.com
Date: Fri, 9 Mar 2007 14:02:51 -0600
Subject: [Microscopy] Re: Carbon evaporation coaters

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Check out Ladd Research for a free standing evaporator for a lot less than
$55K.
http://www.laddresearch.com

FYI: Ladd Research was the only manufacturer or supplier of a rubber L
gasket with a large lip on the bottom to hold the older and thicker walled
glass bell jars. I tried 5-8 suppliers.
They also supplied me with custom made leads on their dual evaporation unit
for retrofitting to my Cooke thermal boat evaporator. I would ask any
supplier about carbon tread flash evaporations and/or accessories.

Disclaimer: I am just a satified customer that got great customer support
and products.

Paul

At 09:32 AM 3/9/07 -0600, you wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

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From: mager-at-interchange.ubc.ca
Date: Fri, 9 Mar 2007 14:03:24 -0600
Subject: [Microscopy] viaWWW: OM Calibration standard Z direction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Javaid,
The method I know of for calibrating the fine focus knob of the optical
microscope is to measure the thickness of a microscope glass slide with a
fine micrometer, put a mark with felt pen on both sides of the slide, offset
from each other a bit, then record the fine focus reading for the focus on
one mark and the fine focus reading for the other mark. Do this for each
objective. The calibration is then based on the micrometer you use.
Hope this helps.
Regards,

Mary Mager
Electron Microscopist
Materials Eng. UBC
#309 - 6350 Stores Road
Vancouver, B.C. V6T 1Z4
Canada
Tel: 604-822-5648
Fax: 604-822-3619
email: mager-at-interchange.ubc.ca

-----Original Message-----
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Email: javaidqazi-at-kemet.com
Name: Javaid Qazi

Title-Subject: [Filtered] OM Calibration standard Z direction

Question: I am looking for a Z direction Calibration standard for an optical
microscope which can do surface profiles, mag ranges from 20 to 1000x.

Please let me know offline what are my options.


Thanks

Javaid


---------------------------------------------------------------------------

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From: vray-at-partbeamsystech.com
Date: Sat, 10 Mar 2007 03:31:29 -0600
Subject: [Microscopy] viaWWW: OM Calibration standard Z direction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Mary,

Are you taking into account index of refraction of the glass, while
measuring fine focus of the marks on both sides of the slide? My guess is
that accuracy of such calibration will depend not only on micrometer, but
also on how accurately the index of refraction is known; it should be close
to 1.2 - 1.5 but probably will vary depending on source of the glass, etc.

You probably could use height standards made for AFM, some even NIST
traceable, there are lots of sources, just Google. Of cause the accuracy of
calibration will still depend on the operator...

Cheers,
Valery

-----Original Message-----
X-from: mager-at-interchange.ubc.ca [mailto:mager-at-interchange.ubc.ca]
Sent: Friday, March 09, 2007 3:04 PM
To: vray-at-partbeamsystech.com

Dear Javaid,
The method I know of for calibrating the fine focus knob of the optical
microscope is to measure the thickness of a microscope glass slide with a
fine micrometer, put a mark with felt pen on both sides of the slide, offset
from each other a bit, then record the fine focus reading for the focus on
one mark and the fine focus reading for the other mark. Do this for each
objective. The calibration is then based on the micrometer you use.
Hope this helps.
Regards,

Mary Mager
Electron Microscopist
Materials Eng. UBC
#309 - 6350 Stores Road
Vancouver, B.C. V6T 1Z4
Canada
Tel: 604-822-5648
Fax: 604-822-3619
email: mager-at-interchange.ubc.ca

-----Original Message-----
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---------------------------------------------------------------------------
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---------------------------------------------------------------------------

Email: javaidqazi-at-kemet.com
Name: Javaid Qazi

Title-Subject: [Filtered] OM Calibration standard Z direction

Question: I am looking for a Z direction Calibration standard for an optical
microscope which can do surface profiles, mag ranges from 20 to 1000x.

Please let me know offline what are my options.


Thanks

Javaid


---------------------------------------------------------------------------

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From: richard-at-torland.demon.co.uk
Date: Sat, 10 Mar 2007 06:22:29 -0600
Subject: [Microscopy] Re: TEM: Free lens control on a JEOL 2011

Contents Retrieved from Microscopy Listserver Archives
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Hi Richard

as Steve points out the OL is turned off in LOW MAG mode. In this case
you use the SAD aperture for contrast not the OL aperture.

Low mag is one of the strengths of this machine.

In Mag Mode if you cannot get good images below 100k you should use
different alpha angles, alpha 3 for the low end and alpha 1 for high
mag, high resolution. This allows you to control beam divergence to suit
the magnification,

Best Regards

Richard
/_____________________________________________________________________________________/
//
/Richard Hey Principal Technical Support Engineer/
/Jeol (UK) Ltd/
//
/Phone: +44 1707377117/
/Fax: +44 1707373255/



protrain-at-emcourses.com wrote:
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} Hi
}
} I have not used the JEOL 2011 but it is a TEM so it must follow standard TEM
} principles.
}
} Instruments usually switch off the objective lens to achieve very low
} magnifications. They use the diffraction lens to focus and gain some
} contrast through the inclusion of the intermediate or diffraction aperture.
}
} Try switching off the objective (some drop to 20% rather then switch off)
} and use the diffraction lens to focus. Balance the remaining lenses to
} reduce distortion if this arrangement causes problems. Introduce the
} diffraction aperture once you have a reasonable image.
}
} The image quality will not be good, probably in excess of 3nm resolution,
} due to the very long focal length required.
}
} Best of luck but if you need more help I am only in Buckingham?
}
} Steve Chapman
} Protrain for EM training & consultancy world wide
} www.emcourses.com
} Tel +44 1280 816512 Fax +44 1280 814007
} Mobile +44 7711 606967
}
}
}
}
} ----- Original Message -----
} X-from: {richard.beanland-at-bookham.com}
} To: {protrain-at-emcourses.com}
} Sent: Wednesday, February 21, 2007 11:56 AM
} Subject: [Microscopy] TEM: Free lens control on a JEOL 2011
}
}
}
} }
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} }
} } Dear list readers,
} } Having struggled with my 'new' microscope for a few years now I
} } am reaching the limits of my knowledge. I am looking at relatively
} } large GaAs devices (} 100um in diameter) and need to be able to take
} } diffraction contrast images of the whole thing. The 2011 is great at
} } magnifications } 100,000x but if I try to get an image at 100x all I see
} } is a tiny bright spot corresponding to the objective aperture. I
} } suspect this means that the objective aperture is nowhere near the back
} } focal plane of the objective lens in low mag mode. Now, I know the kind
} } of image I want was easy to get on my 1979 vintage 120CX, with a 2-stage
} } condenser and one objective lens, whereas this beast has a three stage
} } condenser plus a condenser and objective mini-lenses.
} } This morning I managed to get a reasonable low mag diffraction
} } contrast image by playing with the free lens controls, (essentially
} } turning off some lenses so it behaved more like my old machine). My
} } question is: has anyone done this in a more systematic manner and could
} } give me some directions on which lenses to vary to get what I want? I
} } could just about work it out myself with 3 lenses but I have no idea
} } when there are 5. Not to mention 2 more in the gun, 3 intermediates and
} } a projector, plus alignment lenses...
} }
} } Many TIA
} }
} } Richard
} }
} } ________________________________________
} } Richard Beanland
} } Materials Analysis
} } Bookham
} } Caswell
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} } United Kingdom
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} 15, 27 -- From protrain-at-emcourses.com Wed Feb 21 13:34:56 2007
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6, 19 -- From richard-at-torland.demon.co.uk Sat Mar 10 06:22:29 2007
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From: W8KXR-at-neo.rr.com
Date: Sat, 10 Mar 2007 07:05:35 -0600
Subject: [Microscopy] LM Mecury Burner data - Thanks -

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Gents and Ladies,

Quick note of thanks to those who provided data and advice on power
supply requirements for the HBO 50 Mercury burner. I now have enough
info to build a power supply and am in the search mode for components.

Again, thanks for the help..

Best Regards,

Gene

==============================Original Headers==============================
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From: jquinn-at-www.matscieng.sunysb.edu
Date: Sat, 10 Mar 2007 14:06:16 -0600
Subject: [Microscopy] Carbon evaporation coaters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Paul and listservers:

Duniway sells L-gaskets for Bell Jars.
As does Kurt J. Lesker, NeVac, Huntington,
Key High Vacuum, MDC, CalSeal, GreatGlass, etc.....

regards,

Jim


} From mail-at-ns.microscopy.com Fri Mar 9 14:55:36 2007
} Date: Fri, 9 Mar 2007 14:03:48 -0600
} To: jquinn-at-www.matscieng.sunysb.edu
} From: beaurega-at-westol.com
} Reply-to: beaurega-at-westol.com
} X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com}
} Subject: [Microscopy] Re: Carbon evaporation coaters
} Errors-To: MicroscopyListSpamFilter-at-microscopy.com
} X-lewp: MicroscopyListSpam NAGS
}
}
}
}
} ----------------------------------------------------------------------------
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} ----------------------------------------------------------------------------
}
} Hi,
}
} Check out Ladd Research for a free standing evaporator for a lot less than
} $55K.
} http://www.laddresearch.com
}
} FYI: Ladd Research was the only manufacturer or supplier of a rubber L
} gasket with a large lip on the bottom to hold the older and thicker walled
} glass bell jars. I tried 5-8 suppliers.
} They also supplied me with custom made leads on their dual evaporation unit
} for retrofitting to my Cooke thermal boat evaporator. I would ask any
} supplier about carbon tread flash evaporations and/or accessories.
}
} Disclaimer: I am just a satified customer that got great customer support
} and products.
}
} Paul
}
} At 09:32 AM 3/9/07 -0600, you wrote:
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} } ----------------------------------------------------------------------------
} }
} } A colleague recently told me that a carbon evaporation coater (for
} } sem/epma samples) now lists for over ~US$55K (full scale model-not
} } tabletop, with diffusion pump, no thickness monitor). This is
} } essentially the same unit that I purchased 13 years ago for ~$12K.
} }
} } Does anyone have any idea why the price for such a unit has increased
} } by } 400% in 13 years? Are there still "simple full scale" (not
} } tabletop) models that sell at more "reasonable" prices? (He asked me
} } if I thought an NSF proposal reviewer would look askance as such a
} } high cost of a coater in a proposal...)
} }
} } Thanks.
} }
} } John
} } --
} } ========================================================
} } John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480
} } Cameron Electron Microprobe Lab lab: (608) 265-4798
} } Dept of Geology & Geophysics fax: (608) 262-0693
} } University of Wisconsin home: (608) 274-2245
} } 1215 West Dayton St. email: johnf-at-geology.wisc.edu
} } Madison, WI 53706 amateur radio: WA3BTA
} } Personal http://www.geology.wisc.edu/~johnf/
} } Probe lab http://www.geology.wisc.edu/~johnf/sx51.html
} } Probe Sign Up Calender:
} } http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi
} }
} } "The first rule of all intelligent tinkering is to save every cog and
} } wheel." -- Aldo Leopold
} }
} } "For a successful technology, reality must take precedence over
} } public relations, for Nature cannot be fooled." -- Richard P.
} } Feynman
} }
} } ==============================Original Headers==============================
} } 6, 24 -- From johnf-at-geology.wisc.edu Fri Mar 9 09:31:20 2007
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} } 6, 24 -- To: microscopy-at-microscopy.com
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8, 12 -- From jquinn-at-www.matscieng.sunysb.edu Sat Mar 10 14:06:16 2007
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8, 12 -- Date: Sat, 10 Mar 2007 14:57:49 -0500
8, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu}
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8, 12 -- Subject: Re: [Microscopy] Re: Carbon evaporation coaters
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From: donc-at-asmicro.com
Date: Sat, 10 Mar 2007 22:15:17 -0600
Subject: [Microscopy] T, C, CS and other mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I browsed with interest the links mentioned recently concerning digital
camera adapters for photomicrography. On the vendor web pages, I see
mentioned T-mount, C-mount, CS-mount etc. Can anyone point me to a source
that defines the standard dimensions of various camera mounts such as these?

regards,
Don Chernoff
==================================
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]



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From: Michal.Jarnik-at-fccc.edu
Date: Mon, 12 Mar 2007 09:12:18 -0500
Subject: [Microscopy] Nucleolar markers - antibodies for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

A colleague needs to label nucleoli in mouse thymus and so far I was not
able to find a good antibody (tried a couple). I will be using Tokuyasu
cryosections fixed with 6% formaldehyde. I would prefer commercially
rabbit polyclonal antibody against something really abundant
(fibrillarin, nucleolin or...?). Any tips?

Thanks,

Michal


==============================Original Headers==============================
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From: edelmare-at-muohio.edu
Date: Mon, 12 Mar 2007 09:51:47 -0500
Subject: [Microscopy] Re: T, C, CS and other mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Don:

A quick internet search showed the following:

http://www.k3pgp.org/ccsmount.htm

What is the Difference Between C & CS Mount lens?

The physical difference is the CS mount lens is designed to be
mounted ~5mm closer to the image sensor than a C mount lens. (C-mount
lenses are designed to be mounted 17.526mm in front of the image
sensor vs. 12.5mm for CS-mount.) You can always use a C mount lens on
a CS mount camera by using a 5mm spacer ring (many cameras now have
C/CS selectable adjustment screws or rings). You can never use a CS
mount lens on an older style C mount camera unless you are willing to
physically modify the camera. Cost wise the CS mount lens is much
less expensive since it uses fewer glass elements. Quality of image
is the same. C mounts are becoming less and less popular and are
generally only used on the more telephoto focal lengths such as 25,
50 and 75mm, and bigger zooms.

Both the C and CS mount are 1 inch wide (25.4mm) with 32 threads per
inch (0.03125 inches or 0.79375mm). This dimension comes in handy if
you need to insert a spacer to obtain proper focus. Unscrew the lens
(or unscrew the camera from the mount in the case of telescope use
and count the turns until proper focus is obtained. Multiply the
above dimension by the number of turns to obtain the needed spacer or
washer. (Washers are sometimes used as spacers if there are enough
threads available.) Example: 1.25 turns x 0.79 mm = 0.9875 or ~1 mm.
Many cameras (especially newer ones) have set screws to allow small
adjustments in the distance between the lens and the image sensor.

AND

Definitions of CS-MOUNT on the Web:

* A relatively new industry standard for mounting a lens to a
camera where a 1" X 32 thread is employed and the distance from the
image plane from the shoulder of the lens is 12.52mm. A CS-mount lens
may NOT be used on a C-mount camera.
www.cbcamerica.com/cctvprod/glossary.htm

* "CS-mount" lenses have a flange back distance of 12.5mm vs.
17.526mm for "C-mount" lenses. Because of the shorter back focal
distance, CS-mount lenses can only be used on CS-mount cameras. Your
picture will be out of focus if you use a CS-mount lens on a C-mount
camera.
www.rainbowcctv.com/tech/lensterm.html

T-MOunt

(http://photonotes.org/cgi-bin/entry.pl?id=Tmount)

T-mount.

A threaded (screwmount) lens mount system developed by Tamron for
use with older manual focus lenses and still commonly seen today on
telescope to camera adapters (prime focus photography).

T-mounts are 42mm in diameter with a 0.75 mm thread pitch. They
are thus not compatible with M42 mounts with their 1mm thread pitch,
though they may look the same. You can cause damage to your equipment
if you try to mate the wrong sized components. The "T" stands for
Tamron and not telescope.

cf. astrophotography, M42, prime focus, thread pitch.




On 10 Mar 2007 at 22:16, donc-at-asmicro.com wrote:

}
}
}
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} ----------------------------------------------------------------------------
}
} I browsed with interest the links mentioned recently concerning digital
} camera adapters for photomicrography. On the vendor web pages, I see
} mentioned T-mount, C-mount, CS-mount etc. Can anyone point me to a source
} that defines the standard dimensions of various camera mounts such as these?
}
} regards,
} Don Chernoff
} ==================================
} Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
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Richard E. Edelmann, Ph.D.
EXPO Editor, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu



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From: rcommon-at-msu.edu
Date: Mon, 12 Mar 2007 10:23:54 -0500
Subject: [Microscopy] LR White polymerization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I am new to LR White. The instructions for "Electron Microscopy" strongly
recommends curing 20-24 hrs at 60 degrees plus or minus 2 degrees and warns
of over brittle blocks if this is not followed. But the instructions for
"Electron Microscopic Immunocytochemistry" recommends 50 degrees for 24
hours. My clients want LR White embedding for immunogold staining. I would
appreciate feedback from regular users as to what temperature and curing
time they use. Thanks.

Ralph Common
Michigan State University
Dept. of Physiology


==============================Original Headers==============================
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From: David.Patton-at-uwe.ac.uk
Date: Mon, 12 Mar 2007 10:34:59 -0500
Subject: [Microscopy] LR White polymerization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We got it to polymerise at 52-53 degrees for 24hrs. Worth trying 50
degrees with a blank block. We did not notice any sectioning problems.

dave

-----Original Message-----
X-from: rcommon-at-msu.edu [mailto:rcommon-at-msu.edu]
Sent: 12 March 2007 15:28
To: David Patton


I am new to LR White. The instructions for "Electron Microscopy"
strongly
recommends curing 20-24 hrs at 60 degrees plus or minus 2 degrees and
warns
of over brittle blocks if this is not followed. But the instructions
for
"Electron Microscopic Immunocytochemistry" recommends 50 degrees for 24
hours. My clients want LR White embedding for immunogold staining. I
would
appreciate feedback from regular users as to what temperature and curing
time they use. Thanks.

Ralph Common
Michigan State University
Dept. of Physiology


==============================Original
Headers==============================
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From: lcgould-at-med.cornell.edu
Date: Mon, 12 Mar 2007 10:48:23 -0500
Subject: [Microscopy] Re: LR White polymerization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I polymerize LRW at 50 deg. C for immuno-gold labelling. It cuts
nicely and has good beam stability.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Weill-Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

==============================Original Headers==============================
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From: kraftpiano-at-gmail.com
Date: Mon, 12 Mar 2007 11:11:14 -0500
Subject: [Microscopy] SEM Specimens.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This is a call out to all who might have spare specimens laying around.

We are a middle and high school with an SEM but no sample preparation
equipment. So, until we can get some, I am asking for anyone who may
have some spare samples that they are finished with (Biologicals
especially this year! I would really like a specimen of a neuron of
some variety to show the structure in 3D) or that are hanging around
if they could send them our way so I can have a library of specimens
to use in demonstrations. I can adapt any mount to our mount.

Please email me off list if you have something that you would be
wiling to part with.

Thanks,

Justin A. Kraft
Director
Center for Inquiry-Based Science Education

==============================Original Headers==============================
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From: a.d.mckinnon-at-abdn.ac.uk
Date: Mon, 12 Mar 2007 12:09:27 -0500
Subject: [Microscopy] LR White polymerization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ralph,

Worthwhile bearing in mind the possibility of protein/antigen extraction
during long infiltration periods in LR White and during slow
polymerization at 50 degrees.

I (and others) have found that using LR White accelerator (1.5ul per ml)
and immersing the molds in a crushed ice slush to be a better means of
minimizing crosslinkage of the resin and thereby optimizing antibody
access to the antigen.

A full description of this method and rational can be found in my
immunogold review article in the Journal of Histotechnology/ vol 16, no
3/ Sept 1993.

Get back to me if you have difficulty in accessing this and want further
details.

Regards,

Alastair
Alastair McKinnon
Histology & EM Facility Manager
University of Aberdeen, Institute of Medical Science
Foresterhill, Aberdeen, AB25 2ZD
tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab)
fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk
www.abdn.ac.uk/ims/h-em
-----Original Message-----
X-from: rcommon-at-msu.edu [mailto:rcommon-at-msu.edu]
Sent: 12 March 2007 15:28
To: Mckinnon, Alastair D.


I am new to LR White. The instructions for "Electron Microscopy"
strongly recommends curing 20-24 hrs at 60 degrees plus or minus 2
degrees and warns of over brittle blocks if this is not followed. But
the instructions for "Electron Microscopic Immunocytochemistry"
recommends 50 degrees for 24 hours. My clients want LR White embedding
for immunogold staining. I would appreciate feedback from regular users
as to what temperature and curing time they use. Thanks.

Ralph Common
Michigan State University
Dept. of Physiology


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From: DusevichV-at-umkc.edu
Date: Mon, 12 Mar 2007 13:39:25 -0500
Subject: [Microscopy] Re: AskAMicroscopist: analysis of paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Paper is one of my favorite specimens (the other two are insects and
glass) for the initial demonstration of SEM capabilities to students. It
always has a beautiful structure whether coated or non-coated (observed
in low voltage or environmental mode), and it is very easy to handle.
So, I do not understand why for you "grey scale differences were to
low". If you can send me your images off-line we could discuss them in
more detail.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy


}
} Email: greta.rennings-at-web.de
} Name: Greta Rennings
}
} Organization: KU Leuven
}
} Education: Graduate College
}
} Location: Leuven, Brabant, Belgium
}
} Question: Dear ladies and gentlemen,
}
} do you have an idea which microscopic technique would be suitable for
} analysis of paper?
} Firstly for 3D I did trials with CLSM, which were not truly successful

} as grey scale differences were too low. Other techniques I know would
} require splitting or sectioning, as far as I know- do you have further

} experience?
} Secondly for 2D I tried light microscopy and SEM, but grey scale
} differences were to low here too in order to differentiate between
} components. Could TEM be useful? What could help to get better
} distinguishable features?
}
} Thank you very much in advance for your support!
} Kind regards,
} Greta
}
} --------------------------------------------------------------
} -------------
}
} ==============================Original
} Headers==============================
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From: edelmare-at-muohio.edu
Date: Mon, 12 Mar 2007 15:03:53 -0500
Subject: [Microscopy] Re: EM Field sources

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Once again the list comes through with very valuable information.
Thank you, everyone.

I do not have the answer yet, but as several folks asked when I come
to some conclusions I will post back to the list.


Richard E. Edelmann, Ph.D.
EXPO Editor, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu


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From: gary-at-gaugler.com
Date: Tue, 13 Mar 2007 00:50:44 -0500
Subject: [Microscopy] Re: SEM Specimens.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Good luck.

I have solicited similar requests and will pay for them.

The well is dry.

gary g.


At 08:12 AM 3/12/2007, you wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


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From: nizets2-at-yahoo.com
Date: Tue, 13 Mar 2007 02:59:37 -0500
Subject: [Microscopy] Re: mosquito by SEM - better late than never

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Sorry to reply so late but my private life took most
of my time lately (no I was not in vacations ;-)).
I just wanted to warmly thank all those who
contacted me or replied to my query and apologize in
the case I did not send a personal reply to everybody.

Stephane




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From: Pamela.Lloyd-at-WPAFB.AF.MIL
Date: March 28, 2007
Subject: [Microscopy] MSORV Spring Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

MSORV (the Microscopy Society of the Ohio River Valley) is having their
Spring Meeting on Wednesday, March 28, 2007 in Dayton, OH. The group
has been in an "inactive status" for over 3 years and this will be their
first meeting since the Fall of 2003. This first meeting is going to be
shorter than normal (4 hours) beginning with a mixer/networking social
hour, a Business meeting to discuss what direction and shape the society
will take, and two talks. The Agenda is below. If you have questions
or would like to be placed on our e-mail list to receive directions etc.
please contact either myself or Dave Tomlin. Our e-mail addresses are:
pamela.lloyd-at-wpafb.af.mil or david.tomlin-at-wpafb.af.mil.

Spring MSORV Meeting
Location: UES, Inc.
4401 Dayton-Xenia Rd. Dayton, OH 45432

AGENDA
*3-4 PM Opening Events:

Registration and Membership Applications
Mixer (Sponsored by JEOL, Ltd.)

*4-5 PM Business Meeting:

MSORV:
Role and Value of a Local Society
Needs and Concerns of Members
Format of Future Meetings
Treasurer's Report: Dave Tomlin (UES / WPAFB)
Chair: Matt Chestnut (Procter & Gamble)

*5-7 PM Scientific Presentations:

"WDS X-ray Analysis with Parallel Beam Spectrometers on
Scanning Electron Microscopes" - Alan
Sandborg (EDAX)

"Frontiers in Microscopy for Biological Specimens" -
Wally Ip and Bob Hennigan (UC Medical School)




Pamela F. Lloyd
Materials Engineer
UES, Inc.
AFRL/MLPJE
Bldg. 651, Room 82
3005 Hobson Way
WPAFB, OH 45433
Tel: 937-255-9413
Fax: 937-656-7292
e-mail: pamela.lloyd-at-wpafb.af.mil



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From: rcommon-at-msu.edu
Date: Tue, 13 Mar 2007 10:14:51 -0500
Subject: [Microscopy] LR White polymerization

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Many thanks to everyone who replied, on or off-line, to my question about LR
White polymerization. Most agreed that LR White polymerizes well at 50
degrees in 24 hours, and that sectioning and beam stability are good. There
were, however, some interesting variations suggested. One respondent uses
microwave polymerization. Another uses UV polymerization at 4 degrees.
Another suggested that polymerization at 37 degrees for 3 days might reduce
loss of antigenicity. Several people emphasized the importance of excluding
oxygen and using gelatin capsules when using heat to polymerize, and one
suggested degassing the resin prior to use.

The most interesting suggestions involved using the "cold cure" method. The
instructions that come with the LR White kit recommend not using this method
for immunogold because the exothermic reaction can heat the resin above 60
degrees. But Dr. McKinnon (J. Histotechnology 1993: 16(3)) and others
report superior results with the cold method. Apparently, if the resin can
be kept cold during curing, the low temperature and shorter curing time
reduce loss of antigenicity.

A newer protocol using PTA during processing was also suggested. See Arch
Histol Cytol 68 (5), 337-347 (2005).

Ralph Common
Michigan State University
Dept. of Physiology


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From: DusevichV-at-umkc.edu
Date: Tue, 13 Mar 2007 10:45:45 -0500
Subject: [Microscopy] AskAMicroscopist: analysis of paper

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} -----Original Message-----
} From: Peter Tomic [mailto:peter.tomic-at-renwireless.com]
} Sent: Monday, March 12, 2007 1:57 PM
} To: Dusevich, Vladimir
} Subject: Re: [Microscopy] Re: AskAMicroscopist: analysis of paper
}
} Vladimir,
}
} Just out of curiosity, what do you find interesting about glass?
} Fracture surfaces?
}
} Best regards,
}
} Peter Tomic

For my first demonstration of SEM for students I start usually with
insects. They are supermodels, divas of SEM imaging, they unfailingly
get students excited and prepare them to consume information. Then comes
paper. Nice images of fibers, difference in morphology of writing paper
and filter paper, analysis of filler particles with BSE and EDS. Then I
use three pieces of glass (coverslips): one clean (coated), one "washed"
with tap water and air dried (coated) and one not coated.

On clean glass I start with focusing on dust particle, then move to a
place without any particles, and students are somewhat surprised to see
that there are nothing to look at: just dull monitor with the same
brightness all over. So, I have to remind them, that when we look at
glass with our eyes we do not see any features of its surface, but just
light reflections. It is a good starting point for a brief discussion of
a probe (whether electron beam or light) interaction with specimen and
dependence of a resulting signal on topography.

On "washed" glass students can see a lot of crystals of salts. It leads
to discussion of artifacts in microscopy and importance of proper
specimen preparation.

Not coated glass, of course, is good for demonstration of charging.
Different levels of charging - at 15 kV, when all we see are artifacts,
and at 500 V, when we can get pretty decent image of glass surface (with
dust particles). Finally I demonstrate the extreme case of charging. I
switch off beam, move to a not charged (not previously observed) place
on glass, set high voltage at 15-25 kV and scanning to a spot mode, turn
beam on and charge glass for a few seconds. Then I set voltage to 2-5 kV
and get image of a specimen chamber. Really nice "fish eye" view of a
specimen chamber with an eye in a place of a specimen. I can change
magnification, move image, focusing on some details, such as detectors,
wires, etc. Students, seeing that with simple manipulations we converted
our specimen in a device for observation of specimen chamber, get
excited again, almost as much as when seeing insects. And excitement, I
believe, really helps to remember lesson.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} DusevichV-at-umkc.edu wrote:
} }
} ----------------------------------------------------------------------
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} }
} } Paper is one of my favorite specimens (the other two are insects and
} } glass) for the initial demonstration of SEM capabilities to
} students.
} } It always has a beautiful structure whether coated or non-coated
} } (observed in low voltage or environmental mode), and it is
} very easy to handle.
} } So, I do not understand why for you "grey scale differences were to
} } low". If you can send me your images off-line we could
} discuss them in
} } more detail.
} }
} } Vladimir
} }
} } Vladimir M. Dusevich, Ph.D.
} } Electron Microscope Lab Manager
} } 371 School of Dentistry
} } 650 E. 25th Street
} } Kansas City, MO 64108-2784
} }
} } Phone: (816) 235-2072
} } Fax: (816) 235-5524
} } Web: http://www.umkc.edu/dentistry/microscopy
} }
} }
} } } Email: greta.rennings-at-web.de
} } } Name: Greta Rennings
} } }
} } } Organization: KU Leuven
} } }
} } } Education: Graduate College
} } }
} } } Location: Leuven, Brabant, Belgium
} } }
} } } Question: Dear ladies and gentlemen,
} } }
} } } do you have an idea which microscopic technique would be
} suitable for
} } } analysis of paper?
} } } Firstly for 3D I did trials with CLSM, which were not truly
} } } successful
} }
} } } as grey scale differences were too low. Other techniques I
} know would
} } } require splitting or sectioning, as far as I know- do you have
} } } further
} }
} } } experience?
} } } Secondly for 2D I tried light microscopy and SEM, but grey scale
} } } differences were to low here too in order to differentiate between
} } } components. Could TEM be useful? What could help to get better
} } } distinguishable features?
} } }
} } } Thank you very much in advance for your support!
} } } Kind regards,
} } } Greta
} } }
} } } --------------------------------------------------------------
} } } -------------
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From: jd-at-laddresearch.com
Date: Tue, 13 Mar 2007 12:07:00 -0500
Subject: [Microscopy] Re: Carbon evaporation coaters

Contents Retrieved from Microscopy Listserver Archives
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There are several U.S. manufacturers of floor model (full scale with
diffusion pump) vacuum evaporators and I'd be surprised if they cost
anywhere close to 55K.

The Ladd digital vacuum system, complete with mechanical and
diffusion pumps built in sells for less than 20K. In fact you could
add a turbo and quartz thickness monitor and still be only about 30K.

In the 1960's when we started building our original vacuum systems
the price was about 6K so they really haven't increased that much in 40 years.

John Arnott

Ladd Research
83 Holly Court
Williston, VT 05945

On-line Catalog: www.laddresearch.com

Telephone: 1-802-658-4961 (anywhere)
Toll Free 1-800-451-3406 (US)
Fax: 1-802-660-8859

e-mail: sales-at-laddresearch.com

Disclaimer: Ladd Research sells microscopy supplies and accessories
including vacuum evaporation systems .






---- Original Message ----- From: {beaurega-at-westol.com}
Sent: Friday, March 09, 2007 4:04 PM






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From: bozzola-at-siu.edu
Date: Tue, 13 Mar 2007 16:14:49 -0500
Subject: [Microscopy] Re: Carbon Evaporation Coaters

Contents Retrieved from Microscopy Listserver Archives
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Some evaporation (Brand X) units are quite expensive. I remember
pricing a Brand X evaporator several years ago and nearly passed out
when the quotation came back (over $30K). We decided to keep our
nearly 40 yr old (yet still serviceable) Brand X unit that was
purring alongside our 35 yr old Ladd unit (that cost around $8K). (We
needed two units since one was used for ultraclean evaporative work
and the other by trainees.)

As an aside: several months after I took my first job in Philadelphia
(in 1976), an enormous wooden box arrived in my laboratory. After
unpacking it, I discovered a Ladd evaporator that Margaret Ladd had
kindly sent for me to "try out" for several months (no strings
attached). After 6 months, I passed it along to another investigator.
That certainly made a lasting impression on me. To this day, I have
no idea how she found out that I was a microscopist working at The
Medical College of Pennsylvania. I've never had such an offer since
then.

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
##############################################################

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From: Jerry.L.Lehman-at-NXP.com
Date: Tue, 13 Mar 2007 18:50:16 -0500
Subject: [Microscopy] [FilterviaWWW: Position Available: Failure Analysis Engineer

Contents Retrieved from Microscopy Listserver Archives
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This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
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Email: Jerry.L.Lehman-at-NXP.com
Name: Jerry L. Lehman

Organization: NXP Semiconductors

Title-Subject: [Filtered] Position Available: Failure Analysis Engineer

Question: NXP Semiconductors (Formerly Philips Semiconductors) is expanding its FA Department and is advertising the following position (Job # 2088) at Fishkill, NY, USA:

Job Responsibilities:
Physical and electrical failure analyses and reporting of analogue and digital circuit ICs
Support of design, yield and reliability improvements for products and processes
Support of development projects
Support of quality initiatives including automotive/zero defect
Procedurization and continuous improvement of analysis methods


Experience Profile:
Bachelor/ Master Degree in Electrical Engineering, Materials Science, Communication Engineering, Electronics or Physics
Detailed knowledge in semiconductor physics and technology, analogue and digital signal processing and communication engineering
1-2yrs internship or other experience in Failure Analysis
Some knowledge of TEM and/or FIB is a plus
Fluent English conversation and writing
Good knowledge in standard office tools
Excellent analytical reasoning, communication skills, and organizational skills
Team oriented, customer-centric quality mindset

If interested, please submit your resume thru the following link: http://www.nxp.com/jobs/search/index.html and search by JOB ID # 2088


---------------------------------------------------------------------------


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From: marissagowrie-at-yahoo.com
Date: Tue, 13 Mar 2007 18:50:44 -0500
Subject: [Microscopy] viaWWW: Preparing Pollen Grains for Electron Microscopy

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Email: marissagowrie-at-yahoo.com
Name: Marissa Gowrie

Organization: University of the West Indies St. Augustine

Title-Subject: [Filtered] Preparing Pollen Grains for Electron Microscopy

Question: Hello, I am a post graduate student at the University presently studying the transport of pollen grains in dust. I have been using the Burkard 7 day Spore Sampler to trap the pollen grains and stain them for viewing under the light microscope. I am presently exploring taking electron micrographs of the pollen grains but this would require removing the grains from the greased melinex tape of the sampler and mounting it onto the swab. I have come across the acetolysis process but this does not involve the removal of the pollen grain off the greased melinex tape. Does anyone know of a technique that can be used to remove the pollen from the tape (by dissolving the tape perphaps?) so that they can be placed on the swab?
I look forward to your feedback. Thanks
Marissa

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From: AMCGroup2-at-aol.com
Date: Tue, 13 Mar 2007 18:51:18 -0500
Subject: [Microscopy] viaWWW: TEM image cataloging-archiveing software

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Email: AMCGroup2-at-aol.com
Name: Jim Glossinger

Organization: AMC Group

Title-Subject: [Filtered] TEM image cataloging-archiveing software

Question: Dear all,

We are currently evaluating multi-user, multi-location access Linux-based software products for cataloging-archiving our TEM image database.

Your input is greatly appreciated.

Regards,

Jim Glossinger, Ph.D.
Principal Scientist
AMC Group

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From: kraftpiano-at-gmail.com
Date: Tue, 13 Mar 2007 21:41:43 -0500
Subject: [Microscopy] SEM: Coater home-brew?

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First of all, thank you to everyone who has replied with offers of
specimens! You guys are fantastic!

Now the fun bit:

I was sorting through a closet, and I found the Denton Desk II Carbon
Accessory I have (I believe I offered it up in a previous post as
trade for something useful...)

Anyway, I was wondering if there were a way to use this to do some
carbon coating without the Denton Desk II Base unit. It's got the
power supply, and I can fashion a chamber of some sort over the
business end, but can it be done? I have an ample supply of carbon
rods that came with it.

--Justin A. Kraft
Director
Center for Inquiry-Based Science Education

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From: gary-at-gaugler.com
Date: Tue, 13 Mar 2007 22:46:56 -0500
Subject: [Microscopy] Re: SEM: Coater home-brew?

Contents Retrieved from Microscopy Listserver Archives
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I'm puzzled by this.

I posted for specimens for payment and got no responses.

Should I have posted for them as free? I seek bio
specimens (bacteria, parasites) and I will pay for them.
I do not have CPD and HMDS, etc. capability. If free
is better and more successful than paid, let me know.

gary g.


At 06:43 PM 3/13/2007, you wrote:




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From: jzheng-at-uci.edu
Date: Tue, 13 Mar 2007 23:09:09 -0500
Subject: [Microscopy] best supporting film for protein TEM

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Hi, There,

This may be a simple question for those who are working on protein
observed by TEM. What is the best supporting film for protein TEM work,
silicon monoxide grids or ultra thin carbon film grids? If you can tell me
the reason and the products your are using, it will be very helpful.
Thanks, Jeffery


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From: hyi-at-emory.edu
Date: Wed, 14 Mar 2007 00:17:31 -0500
Subject: [Microscopy] Workshop Announcement

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Dear Researchers:

Emory University School of Medicine Microscopy Core is hosting a
Cryo Technique and Immunogold labeling hands on workshop from Aug. 12
through Aug. 16. Here is some logistical information. Please
contact Hong Yi at hyi-at-emory.edu or (404) 712-8491 for more
information and registration procedure.

1. Date and Curriculum

Aug. 12-13:
Cryo-ultramicrotomy
A new cryo-fixation method
Set up for cryo-substitution
Aug. 14-16:
The properties of gold particles and their protein conjugates.
Theories underlying immunogold labeling protocols.
Silver enhancement of gold particles
Imunogold labeling on a variety of sample preparations for LM.
Immunogold labeling for EM
a. Pre-embedding immunogold labeling using ultrasmall gold conjugates
and silver enhancement.
b. Post-embedding immunogold labeling using conventional colloidal
gold conjugates and ultrasmall gold conjugates.

Numerous biological microscopy techniques will be also
demonstrated during the workshop. Details TBA

2. Main Instructors and Sponsors

Dr. Jan Leunissen, Aurion
Mr. Helmut Gngi, Diatome
Hong Yi, Emory University

Leica Microsystems
Aurion ImmunoGold Reagents
Electron Microscopy Sciences/Diatome
Hitachi High Technologies America, Inc

3. Fees

Session A: Cryo-technique: $500
Session B: Immunogold: $500
Session A and B: $800

Participants can sign up for either the entire workshop
or a particular session of the workshop. If desired, participants
who sign up for cryo-techniques will have additional practice time
after lectures and training during the first two days. Applicants
signing up for both sessions will be given first priority for
enrollment.

4. Participants

The enrollment is open to anyone with interest to learn
regardless of previous experience. However, due to limited space
availability, the number of participants will be limited to 12 for
the cryo-techniques and 20 for immunogold labeling.

5. Lodging

Participants are responsible for making hotel
reservation themselves. The workshop will block a number of rooms at
the following hotels

Villa International: (404) 633-6783, $24/night/person (double
occupancy), or $36/night/person (single occupancy)

This hotel is cozy and clean and often used by Emory to house
temporary or visiting employees. However, TV and phone are only
available in the hotel common room.

Emory Inn: (800) 933-6679, $107/night or higher


Hong Yi
Emory SOM EM

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From: reinhard.rachel-at-biologie.uni-regensburg.de
Date: Wed, 14 Mar 2007 04:59:55 -0500
Subject: [Microscopy] Re: best supporting film for protein TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jeffery,
} What is the best supporting film for protein TEM work,
} silicon monoxide grids or ultra thin carbon film grids? If you can
} tell me
} the reason and the products your are using, it will be very helpful.

in our experience, the best film is a home-made carbon-film (evaporated
onto freshly cleaved mica sheets; by resistence evaporation or even
better by electron gun evaporation), and putting them onto 400 or 600
mesh copper grids.
Before applying the sample: glow-discharge the grid.

We have no experience with silicon monoxide grids.

best regards,
Reinhard Rachel

----------------------
PD Dr. Reinhard Rachel
Universitaet Regensburg
Centre for EM - NWF III -
Lehrstuhl fuer Anatomie
Universitaetsstr. 31
D-93053 Regensburg - Germany
tel +49 941 943 2837, 1720, 1666(TEM)
fax +49 941 943 2868
mail reinhard.rachel-at-biologie.uni-r.de
office: VKL 3.1.29



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From: frank.karl-at-degussa.com
Date: Wed, 14 Mar 2007 06:57:46 -0500
Subject: [Microscopy] Re: viaWWW: Preparing Pollen Grains for Electron Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Marissa,
I have to admit I'm a little surprised. When I was more active with pollen
collecting, I found Ronald Kapp's acetolysis method to dissolve/attack just
about everything accept the pollen exine wall! The problem, is after
acetolysis you can only compare those grains to reference grains which have
been prepared the same way. When I examine air dried pollen from dust, I
see that in many cases it's not a very good match to my acetolysis
collection or to the key in Kapp's book. Still, it's better than nothing.

Not being familiar with the system you are using I can't comment on
solvents, but I would try solvent, followed by dehydrating agents to remove
any water condensed from the evaporative cooling of the solvents and
compare those samples to air dried reference samples.

Have fun and let us know how it turns out for you.

stay safe............Frank





marissagowrie-at-yah
oo.com To: frank.karl-at-degussa.com
cc:
03/13/2007 07:52 Subject: [Microscopy] viaWWW: Preparing Pollen Grains for Electron Microscopy
PM
Please respond to
marissagowrie








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Email: marissagowrie-at-yahoo.com
Name: Marissa Gowrie

Organization: University of the West Indies St. Augustine

Title-Subject: [Filtered] Preparing Pollen Grains for Electron Microscopy

Question: Hello, I am a post graduate student at the University presently
studying the transport of pollen grains in dust. I have been using the
Burkard 7 day Spore Sampler to trap the pollen grains and stain them for
viewing under the light microscope. I am presently exploring taking
electron micrographs of the pollen grains but this would require removing
the grains from the greased melinex tape of the sampler and mounting it
onto the swab. I have come across the acetolysis process but this does not
involve the removal of the pollen grain off the greased melinex tape. Does
anyone know of a technique that can be used to remove the pollen from the
tape (by dissolving the tape perphaps?) so that they can be placed on the
swab?
I look forward to your feedback. Thanks
Marissa

---------------------------------------------------------------------------

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==============================Original Headers==============================
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From: cgarber-at-2spi.com
Date: Wed, 14 Mar 2007 08:49:48 -0500
Subject: [Microscopy] TEM support films for "protein TEM work"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Jeffrey Zheng wrote:
=====================================================
This may be a simple question for those who are working on protein
observed by TEM. What is the best supporting film for protein TEM work,
silicon monoxide grids or ultra thin carbon film grids? If you can tell me
the reason and the products your are using, it will be very helpful.
==================================================
There is a third option, that being the use of silicon nitride membrane
window grids, see URL
http://www.2spi.com/catalog/instruments/silicon-nitride-membrane-window-grids-slot-square.html
If you are not familiar with these grids, the above website page should
answer your questions.

The 20 or 30 nm thick membrane grids are used for this kind of work. At
that thickness, they are very electron transparent and have the added
advantage (over either silicon monoxide/silicon dioxide or ultra thin or
any other carbon filmed grids) of not having any grain structure to
interfere with the visualization of your protein samples.

Another advantage of the silicon nitride membrane window grids is that
there is no "grid sag" as would normally occur with any "filmed"
grid, be it silicon monoxide/silicon dioxide or carbon. With "sag",
one never knows for sure what really is the magnification, or putting it
another way, the sag introduces an error in any ultimate magnification
calculation. The membrane window grids however have outstanding
flatness and therefore (in comparison) no "sag" and this error in
magnification measurement can be eliminated.

Finally, the membrane window grids are inherently more stable in the
electron beam and one can expect lower numbers for image drift under
exposure to the electron beam.

Disclaimer: SPI Supplies offers a full range of silicon nitride and
silicon oxide membrane window grids so we would have a vested interest
in seeing more people using them. The information given above is
somewhat of a composite of what we have been told by present customers.

Chuck
==================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================


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From: tivol-at-caltech.edu
Date: Wed, 14 Mar 2007 11:51:11 -0500
Subject: [Microscopy] Re: best supporting film for protein TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 13, 2007, at 9:09 PM, jzheng-at-uci.edu wrote:

} This may be a simple question for those who are working on protein
} observed by TEM. What is the best supporting film for protein TEM work,
} silicon monoxide grids or ultra thin carbon film grids? If you can
} tell me
} the reason and the products your are using, it will be very helpful.
}
Dear Jeffery,
To an extent, the preferred support film is specimen and technique
dependent. For cryoEM of soluble proteins, a holey film is best, and
one images in the holes, so the image has no film in it. For cryoEM of
membrane proteins, the thin carbon evaporated on mica--either on a
fine-mesh grid or an underlying holey film--works well. I have not
compared the results using thin carbon to those using the Si compounds
Chuck Garber recommends. For negative stained specimens, a continuous
film is necessary, and formvar/carbon works well, since the stain
provides large contrast and the effect of the film is insignificant.
Yours,
Bill Tivol, PhD
EM Scientist
Electron Cryo-Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


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From: jd-at-laddresearch.com
Date: Wed, 14 Mar 2007 12:24:39 -0500
Subject: [Microscopy] Re: best supporting film for protein TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jeffrey,

Without any additional information we would suggest you try the SiO
substrates. We produce a lot of SiO substrates and do not always
know how they are being used but, anecdotally we believe many of them
are being used for protein TEM work.

Are your samples protein crystals?

John Arnott

Ladd Research
83 Holly Court
Williston, VT 05945

On-line Catalog: www.laddresearch.com

Telephone: 1-802-658-4961 (anywhere)
Toll Free 1-800-451-3406 (US)
Fax: 1-802-660-8859

e-mail: sales-at-laddresearch.com

At 12:13 AM 3/14/2007, you wrote:



} ----------------------------------------------------------------------------
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From: jd-at-laddresearch.com
Date: Wed, 14 Mar 2007 12:29:12 -0500
Subject: [Microscopy] Re: best supporting film for protein TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Sorry - I accidentally left off the following disclaimer from my
previous posting:
Ladd Research sells SiO substrates, carbon substrates, support films,
grids and associated products


Hi Jeffrey,

Without any additional information we would suggest you try the SiO
substrates. We produce a lot of SiO substrates and do not always
know how they are being used but, anecdotally we believe many of them
are being used for protein TEM work.

Are your samples protein crystals?

John Arnott

Ladd Research
83 Holly Court
Williston, VT 05945

On-line Catalog: www.laddresearch.com

Telephone: 1-802-658-4961 (anywhere)
Toll Free 1-800-451-3406 (US)
Fax: 1-802-660-8859

e-mail: sales-at-laddresearch.com

At 12:13 AM 3/14/2007, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


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From: marksmsa-at-gmail.com
Date: Wed, 14 Mar 2007 13:44:18 -0500
Subject: [Microscopy] EMMM2007, call for papers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleague,

ELECTRON MICROSCOPY AND MULTISCALE MODELLING,
Moscow, Russia, September 3-7, 2007

http://www.crys.ras.ru/EMMM07/index.en.htm
EMAIL: emmm-07-at-ns.crys.ras.ru

submission of abstracts until 10 May 2007
reduced rate registration until 20 June 2007

This conference, sponsored by the International Union of Crystallography,
will bring together experts in methods of interrogating structures at nano-
and meso-scales, and multiscale materials modelling.

Topics of the conference include:

- electron diffraction imaging
- electron spectroscopy
- density functional methods
- structure of defects and dislocations
- dynamical properties of defects
- dislocation dynamics
- surfaces and defects on surfaces
- structure of grain boundaries
- atomistic modelling of diffusion of defects
- modelling microstructure on nano- and mesoscales
- imaging and spectroscopy of nanostructures
- magnetic effects in materials
- diffuse and small angle scattering
- charge density measurements
- advanced materials for power generation
- applications of electron microscopy
- neutron and X-ray diffraction methods

September is the best time for visiting the city of Moscow to enjoy
the numerous sights and attractions that it has to offer.

We are looking forward to welcoming you to EMMM-07

Prof. Anatoly Avilov
emmm-07-at-ns.crys.ras.ru

Vice Chair, Organizing Committee of EMMM-2007, Institute of
Crystallography, Russian Academy of Sciences, Leninsky Prospect,
Moscow, Russian Federation

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From: eschumacher-at-mccrone.com
Date: Wed, 14 Mar 2007 15:50:09 -0500
Subject: [Microscopy] Meeting: Midwest Microscopy and Microanalysis Society

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Colleagues,

The first 2007 meeting of the Midwest Microscopy and Microanalysis
Society, held jointly with the Biological Imaging Facility at
Northwestern University in Evanston, IL, will be held on Friday, March
23rd. Please follow the link below and click on Meetings for details of
the program and registration information. Note that the M3S website
address has changed:

www.midwestmicroscopy.org

We look forward to seeing you at this and future meetings.

Regards,

Elaine Schumacher
President, Midwest Microscopy and Microanalysis Society
Elaine-at-midwestmicroscopy.org



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From: schramv-at-mail.nih.gov
Date: Wed, 14 Mar 2007 18:18:05 -0500
Subject: [Microscopy] viaWWW: Zeiss workshop in Bethesda (MD) March 27 - 28

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This Question/Comment was submitted to the Microscopy Listserver
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Email: schramv-at-mail.nih.gov
Name: Vincent Schram

Organization: NIH / NICHD

Title-Subject: [Filtered] Zeiss workshop in Bethesda (MD) March 27 - 28

Question: The NICHD Microscopy & Imaging Core is organizing with Carl Zeiss on March 27 and 28 a two-day workshop on deconvolution, long-term incubation and image analysis. The event will be held on the NIH Bethesda campus in Maryland. Anyone using or planning to use a conventional fluorescence microscope is invited to attend.

The workshop includes seminars held in B49 / rm 1A51 and equipment demonstration in B49 / rm 6C72. Please contact Ruth Redman (rredman-at-zeiss.com) to schedule time on the equipment.

The full program is below:


3/27- 10-11AM LECTURE: Wide field Optical Sectioning Techniques for Fluorescent Specimens: Structured Illumination with Apotome and 3D Deconvolution
Demonstrations of these techniques can be scheduled on a hourly basis from 11:30-4:30 that afternoon in B49 / rm 6C72.

3/28- 10-11AM LECTURE: High Speed Physiological Imaging and Analysis with Long Term Incubation
General demonstration immediately following

3/28 1-2PM LECTURE: Automated Image Analysis and Particle Tracking
You are invited to bring your own images for analysis following the lecture.

All lectures to be held in Bldg 49 Room 1A51


---------------------------------------------------------------------------

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From: stevem-at-vt.edu
Date: Wed, 14 Mar 2007 18:18:37 -0500
Subject: [Microscopy] viaWWW: position is open at Va Tech

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Email: stevem-at-vt.edu
Name: Stephen McCartney

Organization: VaTech University

Title-Subject: [Filtered] job posting

Question: The following position is open at Va Tech:

Research Associate/Senior Research Associate, Institute for Critical Technology & Applied Sciences.

Posting Number 070224

Operate instrumentation in the Nanoscale Characterization and Fabrication Laboratory. Maintain and operate the FEI Titan Transmission Electron Microscope (TEM) and the Helios Nanolab 600 Focused Ion Beam (FIB) in the NCFL.

Use this link, click on 'Search and Apply for Faculty Staff and Wage Positions' then use the above Posting number for more info.

http://www.hr.vt.edu/employment/

---------------------------------------------------------------------------

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From: mgb-at-ansto.gov.au
Date: Wed, 14 Mar 2007 22:58:32 -0500
Subject: [Microscopy] viaWWW: TEM aperture questions

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Email: mgb-at-ansto.gov.au
Name: Mark Blackford

Organization: ANSTO

Title-Subject: [Filtered] TEM aperture questions

Question: Hi All,

A colleague is developing a DigitalMicrograph script to help align
our JEOL 2010F TEM. This requires a selected area aperture which is
as close to circular as possible (better than 0.5%) to provide a
reference image.

Can anyone tell me how TEM apertures are manufactured?
How close to a perfect circle can they be made?

Cheers,

Mark Blackford

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From: mark.talbot-at-csiro.au
Date: Wed, 14 Mar 2007 23:26:28 -0500
Subject: [Microscopy] EM & LM Digital database

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Paul,

I have exactly the same question. I am trying to find a database that
will coordinate the cataloguing of images from SEM, confocal and light
microscopes from a large number of regular and temporary users. The
software not only needs to catalogue the images but also accommodate
updateable links to electronic 'notebooks' which describe experimental
details and results (which can be accessed over a network and edited by
people associated with the project). It is also preferable to database
such things as linked PDFs (relevant literature) and data files such as
Excel spreadsheets.

I have so far not managed to find one single program that can do all of
this! There's a great database program called Pax-It, but this is a
little bit user-unfriendly (one important stipulation is that the
program needs to be user-friendly since it would be used by a large mix
of people with a gradation of computer literacy) and so far I can't see
any ability to database together with electronic notebooks. But I may be
asking too much? If anyone could help with this, I would be very happy
to hear from you (and Paul as well).

Thanks,

Mark


Dr. Mark Talbot
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia
mark.talbot-at-csiro.au

ph. 61 (0)2-6246 5256

fax. 61 (0)2-6246 5334

-----Original Message-----
X-from: PWebster-at-hei.org [mailto:PWebster-at-hei.org]
Sent: Thursday, 8 March 2007 4:42 AM
To: Talbot, Mark (PI, Black Mountain)

Hi,

We need a way to catalog stored images (EM & LM) together with
experimental
data (text) for access by in-house researchers as well as off-site
users.

Does anyone know of any commercial products available that would fit our
needs?

We have someone here who would like to try to develop this but it will
be a
waste of time and money if something is already available.

Regards,

Paul.


Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
(213) 273 8026
pwebster-at-hei.org



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From: mnesta-at-ebsciences.com
Date: Thu, 15 Mar 2007 08:24:16 -0500
Subject: [Microscopy] Re: viaWWW: TEM aperture questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Mark,

Molybdenum and platinum apertures are drilled and thin foil apertures
are made with a deposition process using a mask. We regularly perform
SEM inspections on all types of apertures and measure for things like
roundness and concentricity. From our experience, I can tell you that
getting any aperture or apertures that will meet your spec is going to
be hit or miss, but probably more miss. At a maximum 1/2% variation for
roundness, just measuring it presents a challenge.

Sorry I don't have better news.

Best of luck,
Mike Nesta

--
Michael R. Nesta
Managing Director
Energy Beam Sciences, Inc.
Tel: 860 653-0411
Fax: 860 653-0422
MNesta-at-ebsciences.com
www.ebsciences.com
ADDING BRILLIANCE TO YOUR VISION



mgb-at-ansto.gov.au wrote:
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} Email: mgb-at-ansto.gov.au
} Name: Mark Blackford
}
} Organization: ANSTO
}
} Title-Subject: [Filtered] TEM aperture questions
}
} Question: Hi All,
}
} A colleague is developing a DigitalMicrograph script to help align
} our JEOL 2010F TEM. This requires a selected area aperture which is
} as close to circular as possible (better than 0.5%) to provide a
} reference image.
}
} Can anyone tell me how TEM apertures are manufactured?
} How close to a perfect circle can they be made?
}
} Cheers,
}
} Mark Blackford
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
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From: Pascal.Lorentz-at-unibas.ch
Date: Thu, 15 Mar 2007 08:50:38 -0500
Subject: [Microscopy] Axio Vision 3.1

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

we are running the Zeiss Axio Vision 3.1 software on WindowsNT Workstation. Now,
we would like to install Windows XP on our system.
Does anybody know if Axio Vision 3.1 will still run on WinXP?
Any hints will be welcomed.

Best wishes

Pascal

--
Pascal Lorentz
Institute of Biochemistry and Genetics
Department of Clinical-Biological Sciences
University of Basel
Mattenstrasse 28
4058 Basel
Switzerland

Tel: +41 61 695 30 54
Fax: +41 61 267 35 66
E-mail: Pascal.Lorentz-at-unibas.ch

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From: stmccart-at-vt.edu
Date: Thu, 15 Mar 2007 11:56:02 -0500
Subject: [Microscopy] 3d optical microscope

Contents Retrieved from Microscopy Listserver Archives
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Hello: I am looking for information on an optical system that can take 3D
images and also measure X-Y and Z. thanks. Steve

Stephen McCartney
Senior Research Associate
Macromolecules and Interfaces Institute
2108 Hahn Hall
Va Tech
Blacksburg, VA 24061
540-231-9765 - phone
540-231-8517 - FAX


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From: dkoleary-at-verizon.net
Date: Thu, 15 Mar 2007 13:00:35 -0500
Subject: [Microscopy] LM: Polarized Light Microscopy workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Bernard Friedman Memorial Workshop
Polarized Light Microscopy
May 5, 12, 19 & 26, 2007
An advanced course on polarized light microscopy which will cover the following topics:
The nature of polarized light
The origin and interpretation of interference colors
Birefringence and crystal orientation, The Indicatrix
Compensation and variable compensators
Interference figures and their interpretation
The workshop will consist of four consecutive Saturdays of lectures and hands on labs to cover the theoretical and practical aspects of polarized light microscopy. The course instructors include Jan Hinsch formerly of Leica Microsystems, Mary McCann of McCann Imaging, John Reffner of Smiths Detection and N.Y.M.S. Instructor Don O'Leary.
WHEN: May 5, 12, 19 & 26, 2006 from 10 A.M. to 4 P.M.
WHERE: 30 North Mountain Avenue, Montclair, NJ 07042. Phone (973) 744-0043
COST: $395 for N.Y.M.S. members, $425 for non-members (includes membership). Lunch and course materials are included. Checks made out to N.Y.M.S.
WHO: Advanced course for those who have completed "The Use of the Microscope" or are experienced in microscopy and familiar with the theory of its use.
HOW: Register using the form below. Limited to the first 12 registrants.
Return form to Don O'Leary, 10 Sampson Street, Unit 113, Saddle Brook, NJ 07663
FURTHER INFORMATION: Call D. O'Leary (201)368-8849 e-mail dkoleary-at-verizon.net
PLEASE POST
---------------------------------------------------------------------------------------------------------------------------
Registration Form, Polarized Light Microscopy
N.Y.M.S. Member_________________ ($395) Non-Member__________($425)
Name_____________________________________________________________
Address___________________________________________________________
City__________________State____________ZIP______________
Phone (W)_________________________(H)______________________________
e-mail________________________________________


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From: jbpawley-at-wisc.edu
Date: Thu, 15 Mar 2007 13:01:43 -0500
Subject: [Microscopy] RE: EM & LM Digital database

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

As there seems a lot of interest in this, I have coped below a
section on pp 865 of the 3rd Edition of the Handbook of Biological
Confocal Microscopy (Springer, 2005) that might be of some use.

It comes from Chapter 50 on "Databases for Two- and Three-Dimensional
Microscopical
Images in Biology" by Steffen Lindek, Nicholas J. Salmon, and Ernst
H.K. Stelzer.

I trust they will approve.

I personally have no knowledge of this topic.

BioImage
BioImage is a database of multi-dimensional digital images for the
life sciences that at the time of writing is being restructured but
will soon accept image data from a variety of instruments (from
microscopy to satellite remote sensing) relating to all aspects of
biology (from ultrastructural biology to wildlife conservation). In
its first phase (1996-1999), six European research groups and two
industrial partners collaborated on a publicly-funded project investigating
the possibility of storing, in a single database, data generated
from very different specimens (ranging in size from whole
biological organisms down to macromolecules) using very different
microscopes (light, electron, and atomic force microscopes,
etc.).
The aim was to design a database system providing hitherto
unprecedented levels of comparison and data access to emphasize
different, and complementary structural aspects of similar objects.
During the transitional period that followed (2000-2001), the
consortium partners looked for a new orientation of the database
that might lead to a sustainable business model. This led to the
integration of BioImage into the ORIEL project (2002-2004):
Online Research Information Environment for the Life Sciences
(http://www.oriel.org), an EC-funded E-BioSci research project to
integrate internet-based biological information resources for the
scientific community. Because E-BioSci embraces all life sciences
topics, this meant expanding the scope of the BioImage Database
to cover non-microscopical image data such as wild-life photography,
behavioral biology, ecology, etc. At the same time, the
ability to store detailed technical information was reduced.

Cheers,

Jim P.

**********************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building,
FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706
JBPAWLEY-at-WISC.EDU
3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver Canada
Info: http://www.3dcourse.ubc.ca/ Applications still being accepted.
"If it ain't diffraction, it must be statistics." Anon.

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
**********************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building,
FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706
JBPAWLEY-at-WISC.EDU
3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver Canada
Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2007
"If it ain't diffraction, it must be statistics." Anon.

==============================Original Headers==============================
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12, 27 -- Subject: [Microscopy] RE: EM & LM Digital database
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From: jd-at-laddresearch.com
Date: Thu, 15 Mar 2007 14:17:28 -0500
Subject: [Microscopy] Re: viaWWW: TEM aperture questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Mark,

An aperture with a circularity tolerance of 0.5% can be done. We
micro-machine most of the standard EM/FIB apertures because they need
to be burr and flashing free.

With a tolerance of 0.5% you'd have a substantial loss rate so moly,
TA or a metal other than platinum might be a better choice to reduce
the costs.

Depending on your hole size requirements we have other techniques we
could use. We now do apertures/slits for xenon-ion propulsion
satellites. These require extremely tight tolerances and we do them
with a proprietary technique. If you want to send me a drawing we
can let you know it it's possible.

John Arnott

Ladd Research
83 Holly Court
Williston, VT 05945

On-line Catalog: www.laddresearch.com

Telephone: 1-802-658-4961 (anywhere)
Toll Free 1-800-451-3406 (US)
Fax: 1-802-660-8859

e-mail: sales-at-laddresearch.com

Disclaimer: Ladd manufactures apertures, pinholes, micro-holes, slits, etc.

At 12:08 AM 3/15/2007, you wrote:
} ----------------------------------------------------------------------------
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From: mcauliff-at-umdnj.edu
Date: Thu, 15 Mar 2007 14:47:32 -0500
Subject: [Microscopy] rejected as spam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Nestor:

I do not understand why my responses to postings are rejected as spam.

Geoff

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



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From: jpshield-at-uga.edu
Date: Thu, 15 Mar 2007 14:59:22 -0500
Subject: [Microscopy] Re: LR White polymerization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We routinely use 50C for 24 hrs. No problem - must be polymerized in airtight containers though. We've only used gelatin capsules, but I think there is a PCR microfuge tube we tried a long time ago that also worked. There are commercial capsules that are more transparent for UV polymerization.

Any LR White exposed to air (oxygen) will not polymerize, so even in the gelatin capsule, where the lid will have a small bubble, there will be a small amount of liquid to remove.

The other method mentioned above - if the antigen appears to be sensitive to heating and no labeling occurs, you can try to "cold" polymerize by placing the material in capsules that are UV transparent. Place in a container with dry ice and a UV bulb. There are several commercial companies that produce these special beer coolers with fans and lights and reflectors for even polymerization, etc...

John Shields
Univ. of Georgia
EM Lab


---- Original message ----
} Date: Mon, 12 Mar 2007 10:26:14 -0500
} From: rcommon-at-msu.edu
} Subject: [Microscopy] LR White polymerization
} To: jpshield-at-uga.edu
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

706-542-4080

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From: ionsourcerer-at-mac.com
Date: Thu, 15 Mar 2007 15:41:15 -0500
Subject: [Microscopy] Home-brew Coater - The Fun Bit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Don't know why this was just rejected as spam; no bad words, and not
selling anything.

I'll try reformatting it w/o all the prior stuff.

b.


Hi Justin,

Strictly fwiw: Regarding the " Fun Bit ".

There really isn't a lot to these things if my old Hummer II is at
all representative.

When I got mine at a yard sale, I immediately converted it into a
simple sputtering
system for doing some micro-lithographic jewelry I used to make years
ago.
www.refractal.com Eventually, I built a proper system, but still
have the
Hummer around somewhere, and planning to use it someday for teaching.

When all is said and done, a small roughing pump, TC gauge, neon
transformer,
and an egg timer is all you need if you already have the chamber.
Send me a
picture of what you have, and I'll help you with the rest.

You may have most of the bits lying around somewhere already.

Cheers,

b.

Rick Becker
Cluster Sciences, L.L.C.
Borolene Metamaterials
39 Topsfield Rd.
Ipswich, MA 01938 US
978-337-9009
ionsourcerer-at-mac.com

If you don't know where you are going, call it "exploration".
If you don't know what you are doing, call it "research".




On Mar 13, 2007, at 11:51 PM, gary-at-gaugler.com wrote:

--|
--|
--|
--|
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--| America
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--|
--| I'm puzzled by this.
--|
--| I posted for specimens for payment and got no responses.
--|
--| Should I have posted for them as free? I seek bio
--| specimens (bacteria, parasites) and I will pay for them.
--| I do not have CPD and HMDS, etc. capability. If free
--| is better and more successful than paid, let me know.
--|
--| gary g.


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From: jzheng-at-uci.edu
Date: Fri, 16 Mar 2007 00:20:56 -0500
Subject: [Microscopy] Materials Characterization Specialist position at University of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, There,
This position was posted on Feb 7. You still have a very short period of
time to apply it. You should contact Ms. Dorothy Miles instead of me. If
you have applied it, your documents should be properly filed. You will be
informed in due time. Best regards, Jian-Guo

Materials Characterization Specialist
University of California, Irvine
Salary: Commensurate with experience

The University of California, Irvine is seeking a materials
characterization specialist to work in the campus-wide Nanomaterials
Characterization and Fabrication Facility (NCF2). The successful candidate
is expected to have an earned PhD degree in a relevant field, possess an
extensive knowledge in analytical instrumentation (SEM, XRD, AFM, TEM,
FTIR, TGA, DSC) and research implementation, and have rich experience in
sample preparation. He/she should have either extensive knowledge of
techniques and protocols in soft materials characterization, or
demonstrate a desire to acquire such knowledge. The applicant should also
have excellent writing and inter-personal communication skills, and strong
team spirit. Good computing skills are also desirable.
The materials characterization specialist's responsibilities include
carrying out day-to-day operations of analytical equipments including, but
not limited to, SEM, XRD, TEM and AFM/STM. He/she will also be responsible
for (1) maintaining all instruments in good working condition, (2)
training and helping users in the use of analytical equipments, (3)
preparing samples and providing help to users in sample preparation, (4)
assisting in courses related to analytical instrumentation, (5) conducting
service for off-campus and industrial users, (6) maintaining the facility
infrastructure, and (7) carrying out miscellaneous facility-related tasks
assigned by the facility director. The specialist will report to the
director of NCF2. Salary is commensurate with experience. This position
will open immediately and remain open until filled.

Please include in the application the following materials:
Curriculum Vita
2-3 publications
Three letters of recommendation letters (may be submitted shortly after
the submission of the application)

Ms. Dorothy Miles
4100 Calit2 Bldg
Irvine, CA 92697-2800
djmiles-at-uci.edu
ph: 949/824-5178
fax: 949/824-4403

The University of California, Irvine is an equal opportunity employer
committed to excellence through diversity.



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8, 21 -- California, Irvine
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From: keith.morris-at-ucl.ac.uk
Date: Fri, 16 Mar 2007 06:27:10 -0500
Subject: [Microscopy] RE: EM & LM Digital database

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

Has anyone tried the Open Microscopy Environment database system? It is
freeware opensource Linux server based software, and works with ImagJ and
commercial image analysis plugins, plus "the Java-based server system is for
visualizing, managing, and annotating microscope images and metadata". See:

http://openmicroscopy.org/

"OME is a collaborative effort among academic labs and a number of
commercial entities. All OME formats are open and available for use by the
community. All OME source code is available under the GNU library general
public license (LGPL), but OME is designed to interact with new and existing
commercial software".

I saw a presentation on the software a few years ago and it seemed quite
good, but no-one here is really interested in sharing images on this scale.
I wondered if anyone has any experience to share on the suitability of this
open-source software for a Biology optical microscope facility (it seems
literally made for this, but requires a free PC to convert to a Linux
server).

Any thoughts?

Regards

Keith

---------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology
Institute of Ophthalmology
UCL, London EC1V 9EL


-----Original Message-----
X-from: jbpawley-at-wisc.edu [mailto:jbpawley-at-wisc.edu]
Sent: 15 March 2007 18:05
To: keith.morris-at-ucl.ac.uk

Hi all,

As there seems a lot of interest in this, I have coped below a
section on pp 865 of the 3rd Edition of the Handbook of Biological
Confocal Microscopy (Springer, 2005) that might be of some use.

It comes from Chapter 50 on "Databases for Two- and Three-Dimensional
Microscopical
Images in Biology" by Steffen Lindek, Nicholas J. Salmon, and Ernst
H.K. Stelzer.

I trust they will approve.

I personally have no knowledge of this topic.

BioImage
BioImage is a database of multi-dimensional digital images for the
life sciences that at the time of writing is being restructured but
will soon accept image data from a variety of instruments (from
microscopy to satellite remote sensing) relating to all aspects of
biology (from ultrastructural biology to wildlife conservation). In
its first phase (1996-1999), six European research groups and two
industrial partners collaborated on a publicly-funded project investigating
the possibility of storing, in a single database, data generated
from very different specimens (ranging in size from whole
biological organisms down to macromolecules) using very different
microscopes (light, electron, and atomic force microscopes,
etc.).
The aim was to design a database system providing hitherto
unprecedented levels of comparison and data access to emphasize
different, and complementary structural aspects of similar objects.
During the transitional period that followed (2000-2001), the
consortium partners looked for a new orientation of the database
that might lead to a sustainable business model. This led to the
integration of BioImage into the ORIEL project (2002-2004):
Online Research Information Environment for the Life Sciences
(http://www.oriel.org), an EC-funded E-BioSci research project to
integrate internet-based biological information resources for the
scientific community. Because E-BioSci embraces all life sciences
topics, this meant expanding the scope of the BioImage Database
to cover non-microscopical image data such as wild-life photography,
behavioral biology, ecology, etc. At the same time, the
ability to store detailed technical information was reduced.

Cheers,

Jim P.

**********************************************
Prof. James B. Pawley, Ph.
608-263-3147
Room 223, Zoology Research Building,
FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706
JBPAWLEY-at-WISC.EDU
3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver
Canada
Info: http://www.3dcourse.ubc.ca/ Applications still being
accepted.
"If it ain't diffraction, it must be statistics." Anon.

} ---------------------------------------------------------------------------
-
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
**********************************************
Prof. James B. Pawley, Ph.
608-263-3147
Room 223, Zoology Research Building,
FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706
JBPAWLEY-at-WISC.EDU
3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver
Canada
Info: http://www.3dcourse.ubc.ca/ Applications due by March 15,
2007
"If it ain't diffraction, it must be statistics." Anon.

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From: rjharris-at-uwo.ca
Date: Fri, 16 Mar 2007 10:26:29 -0500
Subject: [Microscopy] EM & LM Digital database

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The Open Microscopy Environment (OME) System is being used by labs for their research, a few examples are here: http://www.openmicroscopy.org/use/ OME is being actively developed and while it has several components such as Bio-Formats (http://www.loci.wisc.edu/ome/formats.html) that are being actively used and deployed by non-developer microscopists much of the current OME system is geared towards labs with developer experience. There are active efforts to improve functionality, user installation and performance. One example is the OMERO project which I have pasted below from a recent announcement to the confocal list. For a lab with no development experience looking for a off the shelf solution with the functionality that the original poster requested, the OME system is not currently the answer but will be in the future as it continues to develop (I should note that the focus is now on optical microscopy but we hope to eventually target electron microscopy data as well). The
OME Group gives frequent presentations to update the community on its progress and in fact there will be such a update meeting in Paris on March 30th. I have pasted a announcement about this below. We also have a update meeting about OME in the US at the yearly American Society for Cell Biology Meeting. There are active OME listserves that anyone can join at the www.openmicroscopy.org webpage which also has frequent updates and summaries of meetings. For labs with development interest or companies interested in helping we encourage and need additional OME developer collaborators. Please feel free to contact myself or Jason Swedlow with any questions or comments.

best,
kevin

**********
The OME project is pleased to announce the release of OMERO3.0-
Beta1. OMERO is a Java Enterprise port of the data management and
visualization functions of the perl-based OME Server. The OMERO
Server ships with a JBOSS Application Server, PostgreSQL RDMS, and
uses three client applications: OMERO.insight, OMERO.importer, and
OMERO.admin.

All OMERO resources are available at our newly designed software
download page:

http://openmicroscopy.org/software/

For new initiates, User Guides for OMERO client applications are
available at our MilestoneDownloads page:

http://trac.openmicroscopy.org.uk/omero/wiki/MilestoneDownloads

OMERO installation on OS X is all by Mac packages and should be
automatic. There are manual install instructions for Linux and OS X
geeks.

Please let us know any problems, wishes etc.

**************

Some background and resources:

OMERO3 is a Java EE (formerly J2EE) application designed for the
JBoss application server. OMERO3 runs under Linux and OS X and uses
Hibernate for mapping to a PostgreSQL database (support for MySQL and
Oracle are in progress, but have been demonstrated; contact us if
interested). OMERO3 requires Java1.5. Our testing has all occurred
on Pentium IV or AMD 250-series systems, with 1-2 GB RAM and a Gbit
network connection. The OMERO3 docs page is at:

http://cvs.openmicroscopy.org.uk/tiki/tiki-index.php?page=Omero

Note that this is really for developers.

OMERO.insight is our cross-platform Java client that uses an OMERO3
server. OMERO.insight is our first client; OMERO3 is designed to
support a number of different clients and client platforms (we are
considering our long-term strategies for client environments).
OMERO.insight seems to run well under Linux (so far, tested on RHE3),
Windows XP-SP2, and OS X; the package requires Java1.5. We have
used OMERO.insight on laptops with G4, Intel for Mac, and Intel P4
processors, usually with 0.5 - 1 GB RAM and 100Mbit or Gbit NICs.

OMERO.importer is our data importer. This is a Java client (requires
Java1.5), that currently imports data from a client filesystem into
an OMERO3 server. For import of external file formats, the OMERO
project is using BioFormats- see http://loci.wisc.edu/ome/
formats.html. Currently, OMEROImport supports two file formats--
APLLC DV and MetaMorph's STK. We are of course working to include
OMERO.importer as a component of OMERO.insight, as well as supporting
server-side import. A major goal is the extension of proprietary
file format support in OMERO.importer to include all files supported
in Bio-Formats. We, along with our colleagues at LOCI, are working
hard to expand the range of proprietary file formats supported in
BioFormats. Stay tuned!

What does OMERO do? Mostly, image visualization and image data
management. There is not yet support for any management of image
analysis results and data-- this continues to be one of the hallmarks
of the released OME 2.x server (OME2.6.0 is now available at http://
cvs.openmicroscopy.org.uk; we use this system in the lab for large-
scale image analysis). The OMERO3-Beta1 package provides the ability
to organize image data into Projects and Datasets and also user-
defined CategoryGroups and Categories (see http://openmicroscopy.org/
getting-started/manual_classification.html). In addition, our
server-based Rendering Engine provides image visualization functions
in a remote client environment. Our testing suggests that this that
is a very useful tool for visualising image data in a client-server
environment.

Note that the OME and OMERO Servers are parallel, complementary
projects. They are currently focussing on different function sets--
see http://www.openmicroscopy.org/software/why2servers.html for more
info.

We have recognized the importance of getting feedback on the design,
development, and documentation of OMERO and its clients. OMERO
clients include an automatic bug logging system-- using this helps us
to help you. As the above web pages suggest, we target our work
around a series of milestones, and try to produce packages for
testing these milestones. These should not be seen as finished,
released software, but they are tested and (largely) work as
promised. They are beta and should demonstrate what OMERO can achieve.

Thanks again for your interest and support.

Cheers,

Jason Swedlow
Open Microscopy Environment: http://openmicroscopy.org

********
The OME project is pleased to announce the next OME European Users
Meeting, to be held at the Le Meditel Club in Paris, in association
with Institut Pasteur, March 29/30, 2007. Spencer Shorte, the
Director of the Plateforme d'Imagerie Dynamique at the Institut
Pasteur has kindly agreed to host the meeting.

A draft meeting programme is at the OME website (http://
openmicroscopy.org/latest/euro2007-03.html). We will start the
morning of March 29 with presentations of newly released software,
including the new OME Server2.6.0, the new Java-based OMERO Server
and its clients (see http://openmicroscopy.org/software/), the OME
file formats (http://www.loci.wisc.edu/ome/formats.html & http://
www.loci.wisc.edu/ome/ome-tiff-spec.html), format conversion library
(Bio-Formats; http://loci.wisc.edu/ome/formats.html) and our efforts
to drive usability of our tools (http://www.usableimage.com). The
current programme also includes presentations from Anne Carpenter,
from the Broad Institute on CellProfiler an CellVisualizer (http://
www.cellprofiler.org/), Curtis Rueden from LOCI, Univ of Wisconsin,
Madison (http://www.loci.wisc.edu) and Patrick Courtney (PerkinElmer
LifeSciences).

March 30 will focus on defining goals for critical functionality for
OME and other tools, using breakout groups led by experts in data
models, file formats, usability testing and software development. We
hope to generate a broad roadmap that will define this community's
focus for the next year.

Currently, we have about 30 attendees signed up form US, UK, France,
Switzerland from both academia and commercial institutions.

If you are interested in attending , please send your name,
institution, and contact details to:

Mme Christiane Pacaud (the Imagopole Administrative Assistant),
chrispac-at-pasteur.fr

It will help us if you tell us when you will be arriving and leaving
Paris (to organise airport/train transport, if possible-- no
guarantees, but we will try).

Any requests for presentations or other programme issues can be
addressed to myself and Spencer.

We regret we have no funds to pay for travel or accommodation of
participants, but we will cover coffee and lunch during the meeting.

See you in Paris!

Cheers,

Jason
************

----- Original Message -----
X-from: keith.morris-at-ucl.ac.uk

Hello Mark & Paul
We've just gone through this exercise for the Biotron (here at University of
Western Ontario). Our requirements were for a robust internet accessible
data base that could handle and correlate data in many different formats
(confocal, LM, TEM, SEM, flow cytometery, GS/MS, spectral data, spreadsheet
output from analysis systems and point data such as temperatures, humidity,
etc.) we were also looking for a system that could handle a large ( 10's -
100's of TB) data base information and also allow sophisticated queries and
provide for video, audio and on-line chats all with a (reasonably) user
friendly interface.
We looked at and evaluated a large selection of 'off the shelf' products and
a panoply of custom programmed systems. We chose a product marketed by
Hitachi High Technologies - the Quartz/PCI Wide Area Microscopy suite (no
financial interests in this group - but we are satisfied with both the
product and our relation with Hitachi).
Feel free to contact me off list for more detailed discussions.

Rick
Richard Harris
Manager - Imaging and Data Systems
The Biotron Climate Change Research Facility
The University of Western Ontario
London ON CA
www.biotron.uwo.ca



-----Original Message-----
X-from: mark.talbot-at-csiro.au [mailto:mark.talbot-at-csiro.au]
Sent: Thursday, March 15, 2007 12:30 AM
To: rjharris-at-uwo.ca

Hi Paul,

I have exactly the same question. I am trying to find a database that
will coordinate the cataloguing of images from SEM, confocal and light
microscopes from a large number of regular and temporary users. The
software not only needs to catalogue the images but also accommodate
updateable links to electronic 'notebooks' which describe experimental
details and results (which can be accessed over a network and edited by
people associated with the project). It is also preferable to database
such things as linked PDFs (relevant literature) and data files such as
Excel spreadsheets.

I have so far not managed to find one single program that can do all of
this! There's a great database program called Pax-It, but this is a
little bit user-unfriendly (one important stipulation is that the
program needs to be user-friendly since it would be used by a large mix
of people with a gradation of computer literacy) and so far I can't see
any ability to database together with electronic notebooks. But I may be
asking too much? If anyone could help with this, I would be very happy
to hear from you (and Paul as well).

Thanks,

Mark


Dr. Mark Talbot
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia
mark.talbot-at-csiro.au

ph. 61 (0)2-6246 5256

fax. 61 (0)2-6246 5334

-----Original Message-----
X-from: PWebster-at-hei.org [mailto:PWebster-at-hei.org]
Sent: Thursday, 8 March 2007 4:42 AM
To: Talbot, Mark (PI, Black Mountain)

Hi,

We need a way to catalog stored images (EM & LM) together with
experimental
data (text) for access by in-house researchers as well as off-site
users.

Does anyone know of any commercial products available that would fit our
needs?

We have someone here who would like to try to develop this but it will
be a
waste of time and money if something is already available.

Regards,

Paul.


Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
(213) 273 8026
pwebster-at-hei.org



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From: opmills-at-mtu.edu
Date: Fri, 16 Mar 2007 13:16:20 -0500
Subject: [Microscopy] shorted leadwires - current contrast?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

All,

We have a shorted biomedical leadwire. We've used test equipment to
verify the short. What we would like to do is to visually identify
the location of the short - in the SEM. The leadwire consists of 4
ea ~1mm insulated wires that are then sheathed in insulation. We can
sacrifice the outer insulation but need to retain insulation on the
inner wires. I've imagined that if we power the cables inside the
SEM, we might see current contrast that would divert from one
conductor to another at the short.

Is this experiment as easy as;

building a vacuum feedthrough,
connecting a external bench power supply
wiring the cable to the feedthrough inside the SEM
turn it all on and... WAH LAH! current contrast???

Probably not that easy, so what am I missing?

Owen Mills
Michigan TEch University

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From: bfoster-at-mme1.com
Date: Fri, 16 Mar 2007 14:13:24 -0500
Subject: [Microscopy] Re: 3d optical microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Steve

Can you describe your application a little further? If you need
something that will measure at the macromolecular level, try AFM. If
on a larger scale, both interferometry and confocal microscopy may be
good choices.

Hope this is helpful,
Barbara Foster, President

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com


MME is now scheduling customized, on-site courses through July. Call
us today for details.

P. S.
Need a good general reference or light microscopy text for next
semester? Call us today to learn more about "Optimizing LIght
Microscopy". Copies still available through MME... even for
class-room lots ... and we give quantity discounts. Call Ken Piel at
(972)954-8011.


At 11:31 AM 3/16/2007, stmccart-at-vt.edu wrote:



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From: gary-at-gaugler.com
Date: Fri, 16 Mar 2007 17:04:46 -0500
Subject: [Microscopy] Re: shorted leadwires - current contrast?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Thinking about your problem, I don't think that SEM
is the answer. When you run current through the wires,
it will generate heat, not electrons. If your SEM
has CL, then it probably would work.

I think the only practical (but potentially destructive)
method is to pass enough current to cause the shorted
area to heat up and burn. Or, run as much current as
you can such that the wires do not burn and use an IR
laser temperature gun and scan the wires for a drop in
temperature. Just at this point is past the short.

The amount of current needed is of course dependent on
the resistance of the input end of the wires.

gary g.


At 10:19 AM 3/16/2007, you wrote:




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From: jpchandl-at-mines.edu
Date: Fri, 16 Mar 2007 17:18:38 -0500
Subject: [Microscopy] Re: shorted leadwires - current contrast?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Owen,

Real-time x-ray imaging will give you the answer you need. Many
universities and commercial failure analysis labs have the capability.

--John

John Chandler
Colorado School of Mines
jpchandl-at-mines.edu
303-384-2203

-----Original Message-----
X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com]
Sent: Friday, March 16, 2007 4:11 PM
To: jpchandl-at-mines.edu

Thinking about your problem, I don't think that SEM
is the answer. When you run current through the wires,
it will generate heat, not electrons. If your SEM
has CL, then it probably would work.

I think the only practical (but potentially destructive)
method is to pass enough current to cause the shorted
area to heat up and burn. Or, run as much current as
you can such that the wires do not burn and use an IR
laser temperature gun and scan the wires for a drop in
temperature. Just at this point is past the short.

The amount of current needed is of course dependent on
the resistance of the input end of the wires.

gary g.


At 10:19 AM 3/16/2007, you wrote:




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From: gary-at-gaugler.com
Date: Fri, 16 Mar 2007 17:20:42 -0500
Subject: [Microscopy] Faraday cup readings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi all:

I'm stumped by Faraday cup readings versus Zeiss
Specimen Current Meter (SCM) readings.

For any aperture and any KV, SCM reads 75pA.
If I use the GW EBIC SCM, the values change
as expected. A Keithly picoampmeter also confirms
this.

Why does the Zeiss SCM (Supra 55VP) not change?
It does on a specimen but not into the Faraday cup.

gary g.


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From: kraftpiano-at-gmail.com
Date: Fri, 16 Mar 2007 17:40:22 -0500
Subject: [Microscopy] New SEM donated to our lab- Can you identify the accessory?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

We have a new SEM on the way to us, it's a JEOL JSM-840. All I have
at this point are a couple of pictures, and I am a little flummoxed
about the accessories that are added on this instrument. I recognize
the secondary electron detector, and I believe that there is a
backscatter detector on it, but I'm not sure what the other
accessories are. Also, the photos that I have seen of the basic 840s
don't include a box sitting on top of the column, but this one has it.
It's a silver-ish box with black sides, and is barely visible in one
of the photos, but it is there, at a slight angle. (Which leads me to
the next question- how should it be secured for shipping?)

The whole microscope is being packed by people who don't necessarily
know how to package it. Do any of you have specific suggestions of
what they could do to prevent damage in shipping?

Here is the URL to the photos of the microscope (Hosted on my personal
page which has not been updated in a long time...)
http://www.jkraft.net/JEOL/

Thanks,

Justin A. Kraft
Director
Center for Inquiry-Based Science Education

==============================Original Headers==============================
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6, 26 -- Date: Fri, 16 Mar 2007 18:40:19 -0400
6, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com}
6, 26 -- To: microscopy-at-microscopy.com
6, 26 -- Subject: New SEM donated to our lab- Can you identify the accessory?
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From: jbpawley-at-wisc.edu
Date: Fri, 16 Mar 2007 19:06:50 -0500
Subject: [Microscopy] Re: shorted leadwires - current contrast?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Your 840 has been configured with microprobe capability. I think that this
is called the 840A configuration, but you'll have to check with that. You
have a wavelength dispersive spectrometer, an optical microscope for setting
the focus working distance for the spectrometer, and I believe that there is
a probe current meter there, but I can't make it out well.


-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com

-----Original Message-----
X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
Sent: Friday, March 16, 2007 2:44 PM
To: Walck-at-SouthBayTech.com

Hello all,

We have a new SEM on the way to us, it's a JEOL JSM-840. All I have at this
point are a couple of pictures, and I am a little flummoxed about the
accessories that are added on this instrument. I recognize the secondary
electron detector, and I believe that there is a backscatter detector on it,
but I'm not sure what the other accessories are. Also, the photos that I
have seen of the basic 840s don't include a box sitting on top of the
column, but this one has it.
It's a silver-ish box with black sides, and is barely visible in one of the
photos, but it is there, at a slight angle. (Which leads me to the next
question- how should it be secured for shipping?)

The whole microscope is being packed by people who don't necessarily know
how to package it. Do any of you have specific suggestions of what they
could do to prevent damage in shipping?

Here is the URL to the photos of the microscope (Hosted on my personal page
which has not been updated in a long time...) http://www.jkraft.net/JEOL/

Thanks,

Justin A. Kraft
Director
Center for Inquiry-Based Science Education

==============================Original Headers==============================
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6, 26 -- Date: Fri, 16 Mar 2007 18:40:19 -0400 6, 26 -- From: "Justin Kraft"
{kraftpiano-at-gmail.com} 6, 26 -- To: microscopy-at-microscopy.com 6, 26 --

In the SEM, the secondary electron signal is roughly proportional to
the potential the specimen surface.

Jim P.

Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building,
FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706
JBPAWLEY-at-WISC.EDU
3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver Canada
Info: http://www.3dcourse.ubc.ca/ Applications due by March 20, 2007
"If it ain't diffraction, it must be statistics." Anon.


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**********************************************
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3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver Canada
Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2007
"If it ain't diffraction, it must be statistics." Anon.

==============================Original Headers==============================
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From: kenconverse-at-qualityimages.biz
Date: Sat, 17 Mar 2007 11:36:36 -0500
Subject: [Microscopy] New SEM donated to our lab- Can you identify the

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Justin,
I believe what you have is a JXA-840 (microprobe) with 2 wavelength
spectrometers and a light microscope for establishing the proper WD for
using those spectrometers. The Box on top is an ion pump so that the system
can be used with a LaB6 cathode, if you choose (and have received the
correct wehnelt cap).

Whether it is an 840 or 840A could be told by a look at the electronics
console, but you don't have any pictures of that or the electronics rack
that should be present for the spectometers. I do see the power supply
console in a couple of pictures. Does it include the rotary pump and
possibly compressor?

As far as prepping for shipping, the first thing that jumps out at me is
that the table isn't bolted down! At a minimum you need to get 4
M16x2.00x40mm bolts and 16mm washers to secure the table. You also need to
remove the magnet from the ion pump. Actually, most of the column should be
removed and packed separately unless you're planning to move it only a few
miles at very low speed. I also suspect that the WDS units and light
microscope should be removed. All 3 are complex and fragile.

Is it diffusion pumped or turbo pumped? If it's turbo pumped, the pump must
be removed before shipping. Also the high voltage oil tank in the
electronics console must be removed and the circuit board on its side
protected from damage.

This is going to the W. Palm Beach area of Florida. Where is it currently
located? I would highly recommend sending someone to pack it properly. How
is it going to be shipped? If it's going by truck, it must be "padded van"
unless you're going to have a lot more of it disassembled, crated and put on
pallets.

With more info, I might be of more help.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz



-----Original Message-----
X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
Sent: Friday, March 16, 2007 6:43 PM
To: kenconverse-at-qualityimages.biz

Hello all,

We have a new SEM on the way to us, it's a JEOL JSM-840. All I have at this
point are a couple of pictures, and I am a little flummoxed about the
accessories that are added on this instrument. I recognize the secondary
electron detector, and I believe that there is a backscatter detector on it,
but I'm not sure what the other accessories are. Also, the photos that I
have seen of the basic 840s don't include a box sitting on top of the
column, but this one has it. It's a silver-ish box with black sides, and is
barely visible in one of the photos, but it is there, at a slight angle.
(Which leads me to the next question- how should it be secured for
shipping?)

The whole microscope is being packed by people who don't necessarily know
how to package it. Do any of you have specific suggestions of what they
could do to prevent damage in shipping?

Here is the URL to the photos of the microscope (Hosted on my personal page
which has not been updated in a long time...) http://www.jkraft.net/JEOL/

Thanks,

Justin A. Kraft
Director
Center for Inquiry-Based Science Education

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28, 29 -- From kenconverse-at-qualityimages.biz Sat Mar 17 11:36:36 2007
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From: richard.beanland-at-bookham.com
Date: Mon, 19 Mar 2007 04:03:45 -0500
Subject: [Microscopy] shorted leadwires - current contrast?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Owen,
just to expand on James Pawley's comment - yes, you can see
different _potentials_ (not currents) in SEM (just see what happens when
you have an insulator and it charges up). This is great for looking at
open circuit problems, since you can see a contrast change where the
break in the circuit lies - when you apply a few volts to one side or
the other of the break. Also for seeing surface potentials due to
doping in semiconductors if you can keep the surface clean enough.
However if you have a short circuit everything will be at the same
potential and you won't be able to see the problem. I agree with John
Chandler that real time X-ray radiography is the best approach. If you
still want to try SEM you could connect one end of the circuit to the
specimen stage and image using the specimen current signal. However you
could only do this if the insulation is thin enough to get some of the
electron beam through to the wires, which is probably not possible.

Richard



________________________________________
Richard Beanland
Materials Analysis
Bookham
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________

-----Original Message-----
X-from: opmills-at-mtu.edu [mailto:opmills-at-mtu.edu]
Sent: 16 March 2007 18:18
To: Richard Beanland

All,

We have a shorted biomedical leadwire. We've used test equipment to
verify the short. What we would like to do is to visually identify
the location of the short - in the SEM. The leadwire consists of 4
ea ~1mm insulated wires that are then sheathed in insulation. We can
sacrifice the outer insulation but need to retain insulation on the
inner wires. I've imagined that if we power the cables inside the
SEM, we might see current contrast that would divert from one
conductor to another at the short.

Is this experiment as easy as;

building a vacuum feedthrough,
connecting a external bench power supply
wiring the cable to the feedthrough inside the SEM
turn it all on and... WAH LAH! current contrast???

Probably not that easy, so what am I missing?

Owen Mills
Michigan TEch University

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From: beaurega-at-westol.com
Date: Mon, 19 Mar 2007 11:06:31 -0500
Subject: [Microscopy] Re: shorted leadwires - current contrast?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Owen,

I recently was asked by someone that knew my background in materials,
chemistry and electronics to consult on a problem involving very expensive
printed circuit boards, pro bono. Anyway, I requested to use their real
time video optical microscope to examine the defects and was also given a
brief tour of the production facilities at one point.

One of the pieces of equipment I saw was a transmission X-ray microscope.
It operated just like a transmission electron microscope with a real time
on-line image analysis system and had impressive magnifications. I was
shown a high density 12" by 16" circuit board with an surface mount
technology (SMT) microprocessor socket on it. The large pin grid array
socket pads were soldered underneath and normally invisible. This machine
showed an X-ray view of how every SMT pad looked and if it was isolated,
poorly soldered, non-wetted or shorted. the scan was stopped and I was
shown a complete detailed morphology of a single soldered pad on a CRT as a
demo.
The image analysis process was highly automated and very fast. The
operator could stop the machine and perform a manual inspection of any
solder joint.

This machine surely is the way for you to go. It is nondestructive, fast,
requires no coating, and they could save the real time images. I hope I
gave you a taste of how powerful this system was. The image analysis
software was incredible!

My point is that you can contact a surface mount printed circuit board
manufacturer in your area and see they have this equipment. I am sure they
could look at your wires with a very minimal disruption of their process.
The question is, "Would they accept outside analytical work?" Some places
won't because of legal issues.

Of course you could contact an X-ray microscope manufacturer and ask them
for a demo on your real world sample.
At the time, I was fascinated by how much this "table top" transmission
X-ray microscope could do and how it worked just like my old CM-12 TEM with
real time Quantimet on-line imaging. I never thought to get the
manufacturer's name. It was a commercially built unit though.

Maybe someone on the list could arrange to run a sample for you as a
"professional courtesy" at their technical center. Somebody in the
electronics field and on this list must have access to one of these at
their company or could arrange to run a sample for you. Intel comes to mind.

HTH,

Paul Beauregard
724-834-2247


}
} -----Original Message-----
} X-from: opmills-at-mtu.edu [mailto:opmills-at-mtu.edu]
} Sent: 16 March 2007 18:18
} To: Richard Beanland
} Subject: [Microscopy] shorted leadwires - current contrast?
}
}
}
}
} ------------------------------------------------------------------------
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
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From: edelmare-at-muohio.edu
Date: Mon, 19 Mar 2007 12:19:25 -0500
Subject: [Microscopy] Re: Faraday cup readings

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Gary:

We have a Supra 35VP, and just to let you know our SCM works as you
expect - i.e. like your GW Faraday cup, changes due to KeV and
apertures.

Secondly, I just checked, our SCM reads -2.5pA to -300fA without
the KeV, and Status = underrange (vs Status = Normal).

May not help a lot but at least your know what normal seems to be.



On 16 Mar 2007 at 17:21, gary-at-gaugler.com wrote:

}
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} Hi all:
}
} I'm stumped by Faraday cup readings versus Zeiss
} Specimen Current Meter (SCM) readings.
}
} For any aperture and any KV, SCM reads 75pA.
} If I use the GW EBIC SCM, the values change
} as expected. A Keithly picoampmeter also confirms
} this.
}
} Why does the Zeiss SCM (Supra 55VP) not change?
} It does on a specimen but not into the Faraday cup.
}
} gary g.
}
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Richard E. Edelmann, Ph.D.
EXPO Editor, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
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From: gary-at-gaugler.com
Date: Mon, 19 Mar 2007 12:47:59 -0500
Subject: [Microscopy] Faraday cup readings

Contents Retrieved from Microscopy Listserver Archives
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Thanks for the feedback. The analysis was done
into a Zeiss Faraday cup PN 348342-8055-000.

Zeiss SCM reads 75pA no matter what. GW SCM and Keithly
read according to KV and apertures. But the Zeiss
SCM reads OK onto regular specimens. Odd.

This could be a stage issue. I have the earlier
Klein 5 stage and it leaves a lot to be desired.
It is rather sloppy and will un-initialize at times.
Zeiss said they were going to replace it but that
never happened. My Amray 1910FE had an aperture
in the stage for Faraday cup use and it worked
fine using the external current meters. perhaps
there is something wrong with the Zeiss Faraday
cup. It is new and seems/looks OK. If I can find
another supplier, I will try that and compare.

Since your Zeiss SCM works, it is puzzling at my end.

gary g.



At 09:21 AM 3/19/2007, you wrote:




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From: connellyps-at-mail.nih.gov
Date: Mon, 19 Mar 2007 14:17:13 -0500
Subject: [Microscopy] [Filtered] AskAMicroscopist: RCM MTXL ultra-microtome Light

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (connellyps-at-mail.nih.gov) from
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Monday, March 19, 2007 at 12:56:49
---------------------------------------------------------------------------

Email: connellyps-at-mail.nih.gov
Name: Pat Connelly

Organization: NIH

Education: Graduate College

Location: Bethesda, MD

Question: The light on an RCM MTXL ultra-microtome from 1998 has gone
out. I have replaced the light and althought the fiber optic light
still works there is no light coming from the replaced bulb and the
one removed does not seem to be burnt. Fuses were supplied with the
microtome so I tried to locate them. The Operator's Manual does not
show any fuse locations. There are no fuses in the control box nor
any external fuse ports on the microtome body. Can anyone tell me
where a fuse is and how to get to it. I have not taken an RCM
microtome apart before. The support arm for the binoculars - does
this need to be removed before taking off the housing? Help by phone
or email would be most appreciated.

Thank you,
Pat Connelly
connellyps-at-mail.nih.gov
301-496-3491

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From: pmccurdy-at-lamar.colostate.edu
Date: Mon, 19 Mar 2007 14:28:00 -0500
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Imaging blood cells on

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Email: pmccurdy-at-lamar.colostate.edu
Name: Patrick McCurdy

Organization: Colorado State University

Title-Subject: [Filtered] Imaging blood cells on vinyl

Question: -What does it take to get pictures of cells (WBC, RBC,
platelets) on the
surface of vinyl film and depth filter media?
-Is there an easy way to prepare the samples so the cells aren't
significantly affected?
-What do I need to do to be able to see the cell shape, cell types, and
attachment through this filter depth media that has fibers ~ 2 microns
in diameter?
-What do I need to see the cells piled up in the texture on a vinyl
film?
-Would this all be possible with fluorescence microscope?
-Can I scan a sample at low magnification ~300x to find areas of
interest and look deeper?


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From: lkrupp-at-us.ibm.com
Date: Mon, 19 Mar 2007 18:57:21 -0500
Subject: [Microscopy] ruthenium tetroxide staining recipe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone have a protocol for staining a 2 phase polymer film with
ruthenium tetroxide? I need to try to stain a FIB cross section mounted
on an omniprobe grid, so I'm thinking vapor staining, or possibly 'en
bloc' staining of the film before I FIB it, it's about 30 nm thick on top
of a silicon wafer. The finished cross section is roughly 50 nm or so
sample thickness.

We've already tried vapor staining with OsO4, it has no interaction with
this particular system.

Thank you,
Leslie
_____________________
Leslie Krupp
IBM Almaden Research
650 Harry Road, K19/D1
San Jose, CA 95120-6099
(408) 927-3856

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From: paigelassen-at-yahoo.com
Date: Mon, 19 Mar 2007 21:39:55 -0500
Subject: [Microscopy] [Filtered] AskAMicroscopist: appraisal of vintage microscope

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This Question was submitted to Ask-A-Microscopist by (paigelassen-at-yahoo.com)
from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, March 9, 2007 at 10:09:46
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Email: paigelassen-at-yahoo.com
Name: Paige Lassen

Organization: KCUMB

Education: Graduate College

Location: Newark, NJ, USA

Title: appraisal of vintage microscope

Question: Good morning. I have inherited a microscope and considering selling it. My dad acquired it from a pathologist for medical school back in 1960. If you could provide information about the microscope or direct me to a service that appraises microscopes, it would be much appreciated.
It is an Ernst Leitz GmbH/Wetzlar Laborlux. Serial #(?) is 519 465. Has all original components, slides, box, and papers written in German. Thank you in advance for any recommendations.

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From: pmoeck-at-pdx.edu
Date: Mon, 19 Mar 2007 22:19:12 -0500
Subject: [Microscopy] open position postdoc STEM/EELS, HRTEM, electron diffraction up to

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Postdoc (USA West coast based) for a collaboration between Portland
State University, the University of California at Davis, the University
of Washington at Seattle, and the Pacific NorthWest National Laboratory

Initially for one year at $35,500 plus fringe benefits (health
insurance, retirement benefits, etc.), available from May 1st, 2007,
onwards, extendable up to 3 years by mutual agreement.

------------------------------------------------------------------------

A group of collaborators from Portland State University, the University
of California at Davis, the University of Washington at Seattle, and the
Pacific Northwest National Laboratory is seeking a (male/female) postdoc
for a project on Crystallographic and spectroscopic analyses of
ferromagnetic semiconducting nanoparticle aggregates with Curie
temperatures well above room temperature
{http://www.physics.pdx.edu/%7Epmoeck/projects/Daniels%20project%20short.htm} .

A background in materials physics, materials chemistry, crystallography,
or materials science and engineering is required. Familiarity with
Z-contrast (HAADF) imaging in scanning transmission electron microscopes
and associated electron energy loss spectroscopy are essential. Skills
in high resolution phase-contrast transmission electron microscopy,
electron diffraction and crystallography, Rietveld analysis, and
crystallographic image processing will be appreciated.

The search will be open until the position has been filled. Applications
(CV, list of referees, reasons for coming to the US for applicants from
aboard, list of publications if applicable, etc.) should be sent to any
or both the following collaborators:


Prof. Peter Moeck {http://www.physics.pdx.edu/%7Epmoeck/}

Department of Physics
Portland State University
P.O. Box 751
Portland, Oregon 97207-0751
Tel.: 503 725 4227
Fax: 503 725 2815

e-mail: pmoeck-at-pdx.edu {mailto:pmoeck-at-pdx.edu}

research group: Nanocrystallography Group
{http://www.physics.pdx.edu/%7Egirish/nanogroup/}


free crystallographic on-line databases
{http://nanocrystallography.research.pdx.edu/} with approximately 10,000
structures


Prof. Nigel D. Browning {http://www.chms.ucdavis.edu/research/web/browning}


Department of Chemical Engineering and Materials Science
University of California-Davis
One Shields Ave
Davis, CA 95616

Tel: 530-754-5358 (Davis)
Fax: 530-752-9554 (Davis)
e-mail: nbrowning-at-ucdavis.edu {mailto:nbrowning-at-ucdavis.edu}


research group: Interface Physics Group
{http://www.chms.ucdavis.edu/research/web/browning}



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From: charlesping-at-hotmail.com
Date: Tue, 20 Mar 2007 05:25:38 -0500
Subject: [Microscopy] JEOL 6400F SEM need help for the interface

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi there

Does anyone use the Jeol JSM6400F SEM? I am going to attach a Auger
Spectrumeter to this SEM, but I don't know how to control the SEM, for
example, controling the electron beam scanning, the magnification etc.
There is another EDS instrument connected to this SEM. It use the 'JA2' port
in the SEM,which is a 14 pins interface. This interface looks like a GPIB
one from the outlook.
If anyone know anything concerning this interface and the way to control the
SEM, please give me a hand.

Many thanks
Charles

_________________________________________________________________
Watch free concerts with Pink, Rod Stewart, Oasis and more. Visit MSN
Presents today.
http://music.msn.com/presents?icid=ncmsnpresentstagline&ocid=T002MSN03A07001


==============================Original Headers==============================
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From: nizets2-at-yahoo.com
Date: Tue, 20 Mar 2007 07:03:53 -0500
Subject: [Microscopy] Re: [Filtered] MicroscopyListserverviaWWW: Imaging blood cells on

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi!

Personally I wonder why it would not be possible to do
it with a confocal scanning microscope. Molecular
probes makes whole cell fluorescent markers.
Although I have never done 3D reconstruction with a
confocal microscope, it is pretty well adapted for
this purpose. But you have to consider the penetration
of the laser beam in the filter.

See for ex.:

Rothen-Rutishauser B et al.
Formation of multilayers in the caco-2 cell culture
model: a confocal laser scanning microscopy study.
Pharm Res. 2000 Apr;17(4):460-5.

regards,

Stephane

--- pmccurdy-at-lamar.colostate.edu wrote:

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} Email: pmccurdy-at-lamar.colostate.edu
} Name: Patrick McCurdy
}
} Organization: Colorado State University
}
} Title-Subject: [Filtered] Imaging blood cells on
} vinyl
}
} Question: -What does it take to get pictures of
} cells (WBC, RBC,
} platelets) on the
} surface of vinyl film and depth filter media?
} -Is there an easy way to prepare the samples so the
} cells aren't
} significantly affected?
} -What do I need to do to be able to see the cell
} shape, cell types, and
} attachment through this filter depth media that has
} fibers ~ 2 microns
} in diameter?
} -What do I need to see the cells piled up in the
} texture on a vinyl
} film?
} -Would this all be possible with fluorescence
} microscope?
} -Can I scan a sample at low magnification ~300x to
} find areas of
} interest and look deeper?
}
}
}
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____________________________________________________________________________________
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From: dljones-at-bestweb.net
Date: Tue, 20 Mar 2007 07:48:50 -0500
Subject: [Microscopy] Re: JEOL 6400F SEM need help for the interface

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Charles,

I'm not intimately familiar with the SEM you are referencing, but my first
question is: Does this SEM run in the ultra-high vacuum range? I was not
aware that it could.

dj

On Tue, 20 Mar 2007, charlesping-at-hotmail.com wrote:

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}
} Hi there
}
} Does anyone use the Jeol JSM6400F SEM? I am going to attach a Auger
} Spectrumeter to this SEM, but I don't know how to control the SEM, for
} example, controling the electron beam scanning, the magnification etc.
} There is another EDS instrument connected to this SEM. It use the 'JA2' port
} in the SEM,which is a 14 pins interface. This interface looks like a GPIB
} one from the outlook.
} If anyone know anything concerning this interface and the way to control the
} SEM, please give me a hand.
}
} Many thanks
} Charles
}
} _________________________________________________________________
} Watch free concerts with Pink, Rod Stewart, Oasis and more. Visit MSN
} Presents today.
} http://music.msn.com/presents?icid=ncmsnpresentstagline&ocid=T002MSN03A07001
}
}
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From: colijn.1-at-osu.edu
Date: Tue, 20 Mar 2007 08:45:55 -0500
Subject: [Microscopy] Re: JEOL 6400F SEM need help for the interface

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Charles,

Does your JEOL SEM have a UHV sample chamber? Auger spectrometers
are generally used only in UHV systems. Since the sampling depth is
on the order of a few nm (or less), any surface layers will affect
your results. An old vacuum rule of thumb is that you get 1
monolayer of contamination (oxide, etc.) per second at e-6
torr. Hence at 1e-9 torr, you have ~1000 seconds (20 minutes) before
your pristine surface is no more. Most Auger systems also have Ar
sputtering guns to clean the surface just before the analysis.

The vacuum in most SEM chambers is not adequate to do Auger work.

Cheers,
Henk

At 06:28 AM 03/20/07, you wrote:



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Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


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From: schooley-at-mcn.org
Date: Tue, 20 Mar 2007 10:48:56 -0500
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: appraisal of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Justin,
Unless they are paying for the shipping, you're going to be out a sizeable
chunk of change just in the shipping costs. If someone doesn't spend at
least a few hours properly packing the system for however it is going to be
shipped (and how it will be shipped makes a difference in how it is packed),
you will receive a large anchor for your boat. Unless you have someone at
your end who is experienced and willing to donate their time for getting
this system back up and running, you probably don't have the budget to get
it running if you don't have the budget to have it packed properly.

I hate to be so down on this because I currently support an SEM at a high
school because I firmly believe that having the students have the
opportunity to play with the toys that we get to play with in the real world
is a great way to inspire them to get the education they need. However, in
addition to not seeing some major components to the system in your photos
(you also need someone to evalute what's there), I can tell you that I once
packed a system for a customer on a tight budget. I packed it for shipment
by padded van (which is a much less intensive packing job than for a basic
truck), but they decided to just put it on an Estes 18 wheeler despite what
I had told them. By the time it had gone from PA to CA it was trashed.
Penny wise and pound foolish. And they had PAID for that system!

Unfortunately aquiring a free SEM is an expensive affair unless you can find
someone to do it all properly because THEY are also committed to the
students. It's a sizeable commitment to take on even one SEM pro-bono, but
maybe someone in your area could contact you, if they're interested.
Personally, I would like to see the manufacturers take on a high school
system pro-bono for every x number of field service engineers, but that
probably won't ever happen. (Are you guys paying attention, here?).

I sincerely hope that someone DOES contact you and I also hope that my doom
and gloom is off the mark.

Best wishes
Ken Converse
Owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz



-----Original Message-----
X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com]
Sent: Saturday, March 17, 2007 2:28 PM
To: kenconverse-at-qualityimages.biz

} This Question was submitted to Ask-A-Microscopist by (paigelassen-at-yahoo.com)
} from
} http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
} on Friday, March 9, 2007 at 10:09:46
} Remember to consider the Grade/Age of the student when considering
} the Question
} ---------------------------------------------------------------------------
} Please reply to both paigelassen-at-yahoo.com as well as to the
} Microscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: paigelassen-at-yahoo.com
} Name: Paige Lassen
}
} Organization: KCUMB
}
} Education: Graduate College
}
} Location: Newark, NJ, USA
}
} Title: appraisal of vintage microscope
}
} Question: Good morning. I have inherited a microscope and
} considering selling it. My dad acquired it from a pathologist for
} medical school back in 1960. If you could provide information about
} the microscope or direct me to a service that appraises microscopes,
} it would be much appreciated.
} It is an Ernst Leitz GmbH/Wetzlar Laborlux. Serial #(?) is 519 465.
} Has all original components, slides, box, and papers written in
} German. Thank you in advance for any recommendations.
}
} ---------------------------------------------------------------------------
}
The New York Microscopy Society meets in Montclair, New Jersey; they
have an extensive microscope collection and a lot of expertise.
Contact Don Oleary {donoleary-at-worldnet.att.net} for advice.
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO

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From: gary.m.brown-at-exxonmobil.com
Date: Tue, 20 Mar 2007 11:44:50 -0500
Subject: [Microscopy] Re: ruthenium tetroxide staining recipe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Leslie,

The recipe for preparing ruthenium tetroxide in-situ, as well as other
important tips, follows.

Important safety note: RuO4 vapors is a very strong oxidizer. Therefore,
all work involved in the preparation, use and disposal RuO4 must be done in
a good exhaust hood (} 100 cfm exhaust). Safety glasses with side-shields
(or goggles) and gloves (nitrile is best) is a must.

To make the stain, weigh 0.02g of ruthenium trichloride hydrate
(RuCl3-xH2O, CAS 14898-67-0) into a small (5 ml) vial. Add add 1 ml of
(NaOCl (10-13 %, CAS 7681-52-9) and agitate with a Pasteur pipet until the
RuCl3-xH2O has dissolved. The resulting solution should be deep reddish
brown. Cap the vial immediately. I generally affix the sample to be
stained to the inside of the vial cap and stain for the prescribed amount
of time. The duration of staining for your polymer blend will have to be
determined empirically. To safely dispose the stain following use, reduce
by adding an excess of 10-15% aqueous sodium bisulfite (NaHSO3, CAS
7631-90-5); the reduced solution will become pale green to blue overnight,
at which time it can be properly disposed.

All chemicals are available from Sigma-Aldrich and (presumably) other
vendors. Although I recommend the NaOCl noted above, in a pinch, household
bleach may be used instead of the reagent grade variety. The concentration
of household bleach does decrease with shelf life so be sure to purchase a
new jug with a long expiration date. If you plan to use this procedure
over a period of time, purchase the good stuff and keep it refrigerated.
The advantage of using 10-13% reagent-grade NaOCl is that a usable
concentration of the NaOCl remains in solution over a longer period of
time. Always refrigerate to prolong shelf-life.

Prior to staining, one must first know the composition of the polymer blend
and which phase is preferentially stains by RuO4. You will have to
empirically determine whether staining should be performed en bloc or
following FIB milling.

Several resources on RuO4 staining of polymers are available to you: (1) A
good reference for the selectivity of various stains is "Polymer
Microscopy" by Sawyer and Grubbs (3rd ed.?). (2) Two papers on the subject
are authored by Trent et al. and Montezinos and are referenced in the book.
(3) I included an in-depth appendix on the details of stain preparation and
use in G. M. Brown and J. H. Butler, “New method for the characterization
of domain morphology of polymer blends using ruthenium tetroxide staining
and low voltage scanning electron microscopy (LVSEM)”, Polymer 38 (15),
3937 (1997).

You may write or call me off-line as needed.

Regards,

Gary M. Brown
Microscopy Specialist
ExxonMobil Chemical Company
Baytown Technology & Engineering - West
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: 281 834 2387
fax: 281 834 2395
e-mail: Gary.M.Brown-at-ExxonMobil.com

"Happiness equals reality minus expectations." Tom Magliozzi





lkrupp-at-us.ibm.
com
To
gary.m.brown-at-exxonmobil.com
03/19/07 07:00 cc
PM
Subject
[Microscopy] ruthenium tetroxide
Please respond staining recipe
to
lkrupp-at-us.ibm.
com










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Does anyone have a protocol for staining a 2 phase polymer film with
ruthenium tetroxide? I need to try to stain a FIB cross section mounted
on an omniprobe grid, so I'm thinking vapor staining, or possibly 'en
bloc' staining of the film before I FIB it, it's about 30 nm thick on top
of a silicon wafer. The finished cross section is roughly 50 nm or so
sample thickness.

We've already tried vapor staining with OsO4, it has no interaction with
this particular system.

Thank you,
Leslie
_____________________
Leslie Krupp
IBM Almaden Research
650 Harry Road, K19/D1
San Jose, CA 95120-6099
(408) 927-3856

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From: dljones-at-bestweb.net
Date: Tue, 20 Mar 2007 11:45:51 -0500
Subject: [Microscopy] Re: New SEM donated to our lab- Can you identify the

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Justin,

It has been my experience that Ken is quite knowledgeable on this subject.
His observations and suggestions I would take very seriously. He has
clearly taken some time to look over your pictures and gave you some
excellent comments.

I would also reiterate that, calling it the doom and gloom scenario or
not, it would be very difficult for this instrument to arrive without
damage if it is not packaged correctly.

No matter how it is shipped, it will cost money, likely in the 4 digit
range of dollars. Hopefully for you, in the lower end of that range...

X-from personal experience, I can only suggest that either you find someone
that can package this for you, or do it yourself. I don't know if you have
the knowledge or experience. If you don't, some of us would be willing to
give you free advice as to how better to try and have this instrument
arrive in working order.

I have shipped many kinds of instruments from across the hall to across
the ocean. Moving them presents problems. Proper packaging helps minimize
those problems. Lack of packaging is a recipe for disaster.

I have gotten two SEM's donated to my local high school. In both cases, I
personally went to where they were, packaged them myself and shipped them
myself. It was the only way I could afford to do it. It worked. My
daughter's high school can now give their students college credits
straight from high school.

It is not easy. These instruments are not greeted necessarily with open
arms by the administrators of schools. They are expensive to run, maintain
and require specialized knowledge to operate them.

I really like Ken's comment on having SEM manufacture's donate technical
expertise to schools to get these integrated into high schools. Our
children will do nothing but benefit from this. Our industry will do
nothing but benefit from this. The SEM manufacturers will benefit from
this. It is a win-win situation.

dj

On Tue, 20 Mar 2007, kenconverse-at-qualityimages.biz wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
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} ----------------------------------------------------------------------------
}
} Justin,
} Unless they are paying for the shipping, you're going to be out a sizeable
} chunk of change just in the shipping costs. If someone doesn't spend at
} least a few hours properly packing the system for however it is going to be
} shipped (and how it will be shipped makes a difference in how it is packed),
} you will receive a large anchor for your boat. Unless you have someone at
} your end who is experienced and willing to donate their time for getting
} this system back up and running, you probably don't have the budget to get
} it running if you don't have the budget to have it packed properly.
}
} I hate to be so down on this because I currently support an SEM at a high
} school because I firmly believe that having the students have the
} opportunity to play with the toys that we get to play with in the real world
} is a great way to inspire them to get the education they need. However, in
} addition to not seeing some major components to the system in your photos
} (you also need someone to evalute what's there), I can tell you that I once
} packed a system for a customer on a tight budget. I packed it for shipment
} by padded van (which is a much less intensive packing job than for a basic
} truck), but they decided to just put it on an Estes 18 wheeler despite what
} I had told them. By the time it had gone from PA to CA it was trashed.
} Penny wise and pound foolish. And they had PAID for that system!
}
} Unfortunately aquiring a free SEM is an expensive affair unless you can find
} someone to do it all properly because THEY are also committed to the
} students. It's a sizeable commitment to take on even one SEM pro-bono, but
} maybe someone in your area could contact you, if they're interested.
} Personally, I would like to see the manufacturers take on a high school
} system pro-bono for every x number of field service engineers, but that
} probably won't ever happen. (Are you guys paying attention, here?).
}
} I sincerely hope that someone DOES contact you and I also hope that my doom
} and gloom is off the mark.
}
} Best wishes
} Ken Converse
} Owner
}
} QUALITY IMAGES
} Servicing Scanning Electron Microscopes
} Since 1981
} 474 So. Bridgton Rd.
} Bridgton, ME 04009
} 207-647-4348
} Fax 207-647-2688
} kenconverse-at-qualityimages.biz
} qualityimages.biz
}
}
}
} -----Original Message-----
} X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com]
} Sent: Saturday, March 17, 2007 2:28 PM
} To: kenconverse-at-qualityimages.biz
} Subject: Re: [Microscopy] RE: New SEM donated to our lab- Can you identify
} the accessory?
}
}
} It's in New Jersey right now, and unfortunately we don't have the budget to
} send someone up there to pack it properly. The company who is donating it
} will not spend more than a few minutes prepping it (They aren't making money
} off of it, and they are electronics recyclers, so they just see it as a pile
} of metal) so I need to make instructions as simple and clear as possible,
} without having too much for them to do, or they may just decide to Ebay the
} whole thing.
}
} --Justin.
}
} On 3/17/07, kenconverse-at-qualityimages.biz {kenconverse-at-qualityimages.biz}
} wrote:
} }
} }
} }
} } ----------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
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} }
} ----------------------------------------------------------------------------
} }
} } Justin,
} } I believe what you have is a JXA-840 (microprobe) with 2 wavelength
} } spectrometers and a light microscope for establishing the proper WD
} } for using those spectrometers. The Box on top is an ion pump so that
} } the system can be used with a LaB6 cathode, if you choose (and have
} } received the correct wehnelt cap).
} }
} } Whether it is an 840 or 840A could be told by a look at the
} } electronics console, but you don't have any pictures of that or the
} } electronics rack that should be present for the spectometers. I do
} } see the power supply console in a couple of pictures. Does it include
} } the rotary pump and possibly compressor?
} }
} } As far as prepping for shipping, the first thing that jumps out at me
} } is that the table isn't bolted down! At a minimum you need to get 4
} } M16x2.00x40mm bolts and 16mm washers to secure the table. You also
} } need to remove the magnet from the ion pump. Actually, most of the
} } column should be removed and packed separately unless you're planning
} } to move it only a few miles at very low speed. I also suspect that
} } the WDS units and light microscope should be removed. All 3 are
} } complex and fragile.
} }
} } Is it diffusion pumped or turbo pumped? If it's turbo pumped, the
} } pump must be removed before shipping. Also the high voltage oil tank
} } in the electronics console must be removed and the circuit board on
} } its side protected from damage.
} }
} } This is going to the W. Palm Beach area of Florida. Where is it
} } currently located? I would highly recommend sending someone to pack
} } it properly. How is it going to be shipped? If it's going by truck,
} } it must be "padded van" unless you're going to have a lot more of it
} } disassembled, crated and put on pallets.
} }
} } With more info, I might be of more help.
} }
} } Ken Converse
} } owner
} }
} } QUALITY IMAGES
} } Servicing Scanning Electron Microscopes
} } Since 1981
} } 474 So. Bridgton Rd.
} } Bridgton, ME 04009
} } 207-647-4348
} } Fax 207-647-2688
} } kenconverse-at-qualityimages.biz
} } qualityimages.biz
} }
} }
} }
} } -----Original Message-----
} } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
} } Sent: Friday, March 16, 2007 6:43 PM
} } To: kenconverse-at-qualityimages.biz
} } Subject: [Microscopy] New SEM donated to our lab- Can you identify the
} } accessory?
} }
} }
} }
} }
} }
} } ----------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
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} }
} ----------------------------------------------------------------------------
} }
} } Hello all,
} }
} } We have a new SEM on the way to us, it's a JEOL JSM-840. All I have
} } at this point are a couple of pictures, and I am a little flummoxed
} } about the accessories that are added on this instrument. I recognize
} } the secondary electron detector, and I believe that there is a
} } backscatter detector on it, but I'm not sure what the other
} } accessories are. Also, the photos that I have seen of the basic 840s
} } don't include a box sitting on top of the column, but this one has it.
} } It's a silver-ish box with black sides, and is barely visible in one
} } of the photos, but it is there, at a slight angle. (Which leads me to
} } the next question- how should it be secured for
} } shipping?)
} }
} } The whole microscope is being packed by people who don't necessarily
} } know how to package it. Do any of you have specific suggestions of
} } what they could do to prevent damage in shipping?
} }
} } Here is the URL to the photos of the microscope (Hosted on my personal
} } page which has not been updated in a long time...)
} } http://www.jkraft.net/JEOL/
} }
} } Thanks,
} }
} } Justin A. Kraft
} } Director
} } Center for Inquiry-Based Science Education
} }
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} } 6, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com}
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} } 6, 26 -- Subject: New SEM donated to our lab- Can you identify the
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} } 28, 29 -- From kenconverse-at-qualityimages.biz Sat Mar 17 11:36:36 2007
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} } 28, 29 -- To: {kraftpiano-at-gmail.com} , "MSA Listserver"
} {Microscopy-at-MSA.Microscopy.Com}
} } 28, 29 -- Subject: RE: [Microscopy] New SEM donated to our lab- Can you
} identify the accessory?
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} 19, 30 -- From kenconverse-at-qualityimages.biz Tue Mar 20 10:06:44 2007
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} 19, 30 -- To: "'Justin Kraft'" {kraftpiano-at-gmail.com} ,
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} 19, 30 -- Subject: RE: [Microscopy] RE: New SEM donated to our lab- Can you identify the accessory?
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From: bozzola-at-siu.edu
Date: Tue, 20 Mar 2007 14:01:00 -0500
Subject: [Microscopy] Re: New SEM donated to our lab- Can you identify

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Ken,

You make a VERY IMPORTANT point here: "Getting the instrument is the
easy part. Getting it up and running consistently well takes a lot of
work."

In my SEM course, I always take time to point out to my students that
they may be in a situation where someone wants to donate an EM to
their school. No matter how well it is working when the donation is
accepted, there is no guarantee that it will ever work properly in
the new location unless professionals are involved in the packing,
shipping and installation. I always recommend having $5-10K
available. Hopefully, it will never be needed. My analogy is: "It's
like having a baby. The easy part is producing the baby. Caring for
it is a labor (hopefully of love)."

JB


} Justin,
} Unless they are paying for the shipping, you're going to be out a sizeable
} chunk of change just in the shipping costs. If someone doesn't spend at
} least a few hours properly packing the system for however it is going to be
} shipped (and how it will be shipped makes a difference in how it is packed),
} you will receive a large anchor for your boat. Unless you have someone at
} your end who is experienced and willing to donate their time for getting
} this system back up and running, you probably don't have the budget to get
} it running if you don't have the budget to have it packed properly.
}
} I hate to be so down on this because I currently support an SEM at a high
} school because I firmly believe that having the students have the
} opportunity to play with the toys that we get to play with in the real world
} is a great way to inspire them to get the education they need. However, in
} addition to not seeing some major components to the system in your photos
} (you also need someone to evalute what's there), I can tell you that I once
} packed a system for a customer on a tight budget. I packed it for shipment
} by padded van (which is a much less intensive packing job than for a basic
} truck), but they decided to just put it on an Estes 18 wheeler despite what
} I had told them. By the time it had gone from PA to CA it was trashed.
} Penny wise and pound foolish. And they had PAID for that system!
}
} Unfortunately aquiring a free SEM is an expensive affair unless you can find
} someone to do it all properly because THEY are also committed to the
} students. It's a sizeable commitment to take on even one SEM pro-bono, but
} maybe someone in your area could contact you, if they're interested.
} Personally, I would like to see the manufacturers take on a high school
} system pro-bono for every x number of field service engineers, but that
} probably won't ever happen. (Are you guys paying attention, here?).
}
} I sincerely hope that someone DOES contact you and I also hope that my doom
} and gloom is off the mark.
}
} Best wishes
} Ken Converse
} Owner
}
} QUALITY IMAGES
} Servicing Scanning Electron Microscopes
} Since 1981
} 474 So. Bridgton Rd.
} Bridgton, ME 04009
} 207-647-4348
} Fax 207-647-2688
} kenconverse-at-qualityimages.biz
} qualityimages.biz
}
}
}
} -----Original Message-----
} X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com]
} Sent: Saturday, March 17, 2007 2:28 PM
} To: kenconverse-at-qualityimages.biz
} Subject: Re: [Microscopy] RE: New SEM donated to our lab- Can you identify
} the accessory?
}
}
} It's in New Jersey right now, and unfortunately we don't have the budget to
} send someone up there to pack it properly. The company who is donating it
} will not spend more than a few minutes prepping it (They aren't making money
} off of it, and they are electronics recyclers, so they just see it as a pile
} of metal) so I need to make instructions as simple and clear as possible,
} without having too much for them to do, or they may just decide to Ebay the
} whole thing.
}
} --Justin.
}
} On 3/17/07, kenconverse-at-qualityimages.biz {kenconverse-at-qualityimages.biz}
} wrote:
} }
} }
} }
} } ----------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ----------------------------------------------------------------------------
} }
} } Justin,
} } I believe what you have is a JXA-840 (microprobe) with 2 wavelength
} } spectrometers and a light microscope for establishing the proper WD
} } for using those spectrometers. The Box on top is an ion pump so that
} } the system can be used with a LaB6 cathode, if you choose (and have
} } received the correct wehnelt cap).
} }
} } Whether it is an 840 or 840A could be told by a look at the
} } electronics console, but you don't have any pictures of that or the
} } electronics rack that should be present for the spectometers. I do
} } see the power supply console in a couple of pictures. Does it include
} } the rotary pump and possibly compressor?
} }
} } As far as prepping for shipping, the first thing that jumps out at me
} } is that the table isn't bolted down! At a minimum you need to get 4
} } M16x2.00x40mm bolts and 16mm washers to secure the table. You also
} } need to remove the magnet from the ion pump. Actually, most of the
} } column should be removed and packed separately unless you're planning
} } to move it only a few miles at very low speed. I also suspect that
} } the WDS units and light microscope should be removed. All 3 are
} } complex and fragile.
} }
} } Is it diffusion pumped or turbo pumped? If it's turbo pumped, the
} } pump must be removed before shipping. Also the high voltage oil tank
} } in the electronics console must be removed and the circuit board on
} } its side protected from damage.
} }
} } This is going to the W. Palm Beach area of Florida. Where is it
} } currently located? I would highly recommend sending someone to pack
} } it properly. How is it going to be shipped? If it's going by truck,
} } it must be "padded van" unless you're going to have a lot more of it
} } disassembled, crated and put on pallets.
} }
} } With more info, I might be of more help.
} }
} } Ken Converse
} } owner
} }
} } QUALITY IMAGES
} } Servicing Scanning Electron Microscopes
} } Since 1981
} } 474 So. Bridgton Rd.
} } Bridgton, ME 04009
} } 207-647-4348
} } Fax 207-647-2688
} } kenconverse-at-qualityimages.biz
} } qualityimages.biz
} }
} }
} }
} } -----Original Message-----
} } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
} } Sent: Friday, March 16, 2007 6:43 PM
} } To: kenconverse-at-qualityimages.biz
} } Subject: [Microscopy] New SEM donated to our lab- Can you identify the
} } accessory?
} }
} }
} }
} }
} }
} } ----------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ----------------------------------------------------------------------------
} }
} } Hello all,
} }
} } We have a new SEM on the way to us, it's a JEOL JSM-840. All I have
} } at this point are a couple of pictures, and I am a little flummoxed
} } about the accessories that are added on this instrument. I recognize
} } the secondary electron detector, and I believe that there is a
} } backscatter detector on it, but I'm not sure what the other
} } accessories are. Also, the photos that I have seen of the basic 840s
} } don't include a box sitting on top of the column, but this one has it.
} } It's a silver-ish box with black sides, and is barely visible in one
} } of the photos, but it is there, at a slight angle. (Which leads me to
} } the next question- how should it be secured for
} } shipping?)
} }
} } The whole microscope is being packed by people who don't necessarily
} } know how to package it. Do any of you have specific suggestions of
} } what they could do to prevent damage in shipping?
} }
} } Here is the URL to the photos of the microscope (Hosted on my personal
} } page which has not been updated in a long time...)
} } http://www.jkraft.net/JEOL/
} }
} } Thanks,
} }
} } Justin A. Kraft
} } Director
} } Center for Inquiry-Based Science Education
} }
} } ==============================Original
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} Can you identify the accessory?
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From: keith.morris-at-ucl.ac.uk
Date: Tue, 20 Mar 2007 14:23:07 -0500
Subject: [Microscopy] [Filtered] AskAMicroscopist: appraisal of vintage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Paige,

Ernst Leitz GmbH is now called 'Leica'. Leica is now three companies: Leica
Camera AG, which produces cameras; Leica Geosystems AG which produces
geosurvey equipment; and Leica Microsystems GmbH, which produces
microscopes.

Leica Microsystems GmbH is the owner of the Leica brand, and grants licenses
to Leica Camera AG and Leica Geosystems. I've not seen any new microscopes
under the Leitz brand recently, they all seem to now go under the Leica
name, although there are many modernish equivalents of your Laborlux about,
e.g.

http://www.saulresearch.com/photomicroscopy.htm

Which seem to be from the 1980s.

Yours is no doubt an older variety of the brand, and they kept the Laborlux
name for new models. e.g.

http://www.microscopesfromnightingale.com/cgi-bin/shop/pid_55.htm

http://www.microscopen-specialist.nl/fs_polarisatie.html?=polarisatie-micros
coop.html

Microscopes can come with a lot of optional accessories for illumination,
contrast enhancement etc.. so yours may or may not have these (and this
affects value as all parts are rather expensive on a microscope and add up).
Even the magnifying objectives are bought separately (as they are expensive
and you buy those most suited to your needs and pocket). www.leica.com
[Microsystems] should be able to provide you with some details, like the
year of manufacture, and perhaps even a pamphlet/brochure scan. They no
doubt have an archivist/museum but possibly not much free time pass on info.

If you want the microscope cleaned/serviced there will be local microscope
independents who specialise in this sort of thing - these undercut the
manufacturers who are very expensive (300+ just to arrive). However all are
geared towards laboratories who have deeper pockets. As mentioned by
Caroline, a local microscope society will be a far better option, as these
enthusiasts are keen to help and have a sounder knowledge of old (cheap) kit
than a professional microscopist. All our systems are over 10,000 and many
mainstay systems are 250,000 (e.g. 'confocal' microscopes with multiple
lasers as the light source and all controlled by PCs). Thus we aren't a lot
of help in this case, as few of us will have held or used a microscope like
yours (it's even before my time), although we may have seen similar in the
lab cupboard as a youth.

If the microscope is serviceable you may just need to clean the eyepieces
and objectives if dirty, and perhaps lubricate the mechanical parts (those
microscope society chaps can advise here). This needs some care and
specialised equipment/fluids though and I can advise if you want to clean
the parts yourself for personal use. Otherwise I would sell it 'as is' -
microscopes are as often bought as much for display as a working scientific
instrument, although yours has the potential to be used for fun amateur
study if clean/working (and similar ones are still used professionally in
the field rather than in the microscope lab). If sold 'as is', the buyer
then takes responsibility for cleaning (and any damage that results).

If you want to sell, Leitz is a quality brand that will attract a premium.
There are many examples of Leitz microscopes second hand on the web and on
ebay. Have a look and get a feel for the going price (dealers may make
extortionate mark-ups though). Also track a few Leitz microscopes through
ebay.com (there's no rush to sell). I would expect the microscope to be
easily valued at well over 200, and rather more if it is in excellent
serviceable condition and is complete. It will have probably cost several
thousand pounds new in today's money (or 100 in 1950's money). Antique high
quality microscopes (older than 1900, attract a serious collectors attention
and a premium, but a 1950's model is often a little less valuable as it has
less pretty brass and is just a bit large to put in a display cabinet. But
like most things, it just depends on it's condition and how much someone is
prepared to pay for it on the day.

Regards

Keith

---------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology
Institute of Ophthalmology
UCL, London EC1V 9EL

There are a few sites on the web interested in old microscopes, so have a
search. I found a few:

http://www.xmission.com/~psneeley/Personal/Microscope.htm

http://www.smecc.org/rca_emt_tabletop.htm (the mind boggles)

https://www.minresco.com/microscopes/micscope.htm

http://www.bartsandthelondon.org.uk/aboutus/recentacqusitions2.asp


There is a Leitz Laborlux 12 POL Microscope Brouchure and a more modern
Labolux microscope on ebay.com at the moment that looks quite decent (no
takers for the $1,300 price tag yet).


-----Original Message-----
X-from: paigelassen-at-yahoo.com [mailto:paigelassen-at-yahoo.com]
Sent: 20 March 2007 02:44
To: keith.morris-at-ucl.ac.uk

This Question was submitted to Ask-A-Microscopist by (paigelassen-at-yahoo.com)

from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on
Friday, March 9, 2007 at 10:09:46
Remember to consider the Grade/Age of the student when considering the
Question
---------------------------------------------------------------------------
Please reply to both paigelassen-at-yahoo.com as well as to the Microscopy
Listserver
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Email: paigelassen-at-yahoo.com
Name: Paige Lassen

Organization: KCUMB

Education: Graduate College

Location: Newark, NJ, USA

Title: appraisal of vintage microscope

Question: Good morning. I have inherited a microscope and considering
selling it. My dad acquired it from a pathologist for medical school back in
1960. If you could provide information about the microscope or direct me to
a service that appraises microscopes, it would be much appreciated.
It is an Ernst Leitz GmbH/Wetzlar Laborlux. Serial #(?) is 519 465. Has all
original components, slides, box, and papers written in German. Thank you in
advance for any recommendations.

---------------------------------------------------------------------------

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From: walck-at-southbaytech.com
Date: Tue, 20 Mar 2007 15:22:30 -0500
Subject: [Microscopy] Re: New SEM donated to our lab- Can you identify

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

You all have to hire the guys that moved the microscope on the TV show,
"Crossing Jordan".

In the story line, they had moved the EM out of the lab for cost cutting and
had it in the basement. When they needed it, the boss told them to "go get
my microscope" and they had it up and running in less than an hour.

Well, I thought it was funny!


-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com

-----Original Message-----
X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
Sent: Tuesday, March 20, 2007 11:06 AM
To: Walck-at-SouthBayTech.com

Ken,

You make a VERY IMPORTANT point here: "Getting the instrument is the easy
part. Getting it up and running consistently well takes a lot of work."

In my SEM course, I always take time to point out to my students that they
may be in a situation where someone wants to donate an EM to their school.
No matter how well it is working when the donation is accepted, there is no
guarantee that it will ever work properly in the new location unless
professionals are involved in the packing, shipping and installation. I
always recommend having $5-10K available. Hopefully, it will never be
needed. My analogy is: "It's like having a baby. The easy part is producing
the baby. Caring for it is a labor (hopefully of love)."

JB


} Justin,
} Unless they are paying for the shipping, you're going to be out a
} sizeable chunk of change just in the shipping costs. If someone
} doesn't spend at least a few hours properly packing the system for
} however it is going to be shipped (and how it will be shipped makes a
} difference in how it is packed), you will receive a large anchor for
} your boat. Unless you have someone at your end who is experienced and
} willing to donate their time for getting this system back up and
} running, you probably don't have the budget to get it running if you don't
have the budget to have it packed properly.
}
} I hate to be so down on this because I currently support an SEM at a
} high school because I firmly believe that having the students have the
} opportunity to play with the toys that we get to play with in the real
} world is a great way to inspire them to get the education they need.
} However, in addition to not seeing some major components to the system
} in your photos (you also need someone to evalute what's there), I can
} tell you that I once packed a system for a customer on a tight budget.
} I packed it for shipment by padded van (which is a much less intensive
} packing job than for a basic truck), but they decided to just put it on
} an Estes 18 wheeler despite what I had told them. By the time it had gone
from PA to CA it was trashed.
} Penny wise and pound foolish. And they had PAID for that system!
}
} Unfortunately aquiring a free SEM is an expensive affair unless you can
} find someone to do it all properly because THEY are also committed to
} the students. It's a sizeable commitment to take on even one SEM
} pro-bono, but maybe someone in your area could contact you, if they're
interested.
} Personally, I would like to see the manufacturers take on a high school
} system pro-bono for every x number of field service engineers, but that
} probably won't ever happen. (Are you guys paying attention, here?).
}
} I sincerely hope that someone DOES contact you and I also hope that my
} doom and gloom is off the mark.
}
} Best wishes
} Ken Converse
} Owner
}
} QUALITY IMAGES
} Servicing Scanning Electron Microscopes Since 1981
} 474 So. Bridgton Rd.
} Bridgton, ME 04009
} 207-647-4348
} Fax 207-647-2688
} kenconverse-at-qualityimages.biz
} qualityimages.biz
}
}
}
} -----Original Message-----
} X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com]
} Sent: Saturday, March 17, 2007 2:28 PM
} To: kenconverse-at-qualityimages.biz
} Subject: Re: [Microscopy] RE: New SEM donated to our lab- Can you
} identify the accessory?
}
}
} It's in New Jersey right now, and unfortunately we don't have the
} budget to send someone up there to pack it properly. The company who
} is donating it will not spend more than a few minutes prepping it (They
} aren't making money off of it, and they are electronics recyclers, so
} they just see it as a pile of metal) so I need to make instructions as
} simple and clear as possible, without having too much for them to do,
} or they may just decide to Ebay the whole thing.
}
} --Justin.
}
} On 3/17/07, kenconverse-at-qualityimages.biz
} {kenconverse-at-qualityimages.biz}
} wrote:
} }
} }
} }
} }
} } ---------------------------------------------------------------------
} } -
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------
} -----
} }
} } Justin,
} } I believe what you have is a JXA-840 (microprobe) with 2 wavelength
} } spectrometers and a light microscope for establishing the proper WD
} } for using those spectrometers. The Box on top is an ion pump so that
} } the system can be used with a LaB6 cathode, if you choose (and have
} } received the correct wehnelt cap).
} }
} } Whether it is an 840 or 840A could be told by a look at the
} } electronics console, but you don't have any pictures of that or the
} } electronics rack that should be present for the spectometers. I do
} } see the power supply console in a couple of pictures. Does it
} } include the rotary pump and possibly compressor?
} }
} } As far as prepping for shipping, the first thing that jumps out at
} } me is that the table isn't bolted down! At a minimum you need to
} } get 4 M16x2.00x40mm bolts and 16mm washers to secure the table. You
} } also need to remove the magnet from the ion pump. Actually, most of
} } the column should be removed and packed separately unless you're
} } planning to move it only a few miles at very low speed. I also
} } suspect that the WDS units and light microscope should be removed.
} } All 3 are complex and fragile.
} }
} } Is it diffusion pumped or turbo pumped? If it's turbo pumped, the
} } pump must be removed before shipping. Also the high voltage oil tank
} } in the electronics console must be removed and the circuit board on
} } its side protected from damage.
} }
} } This is going to the W. Palm Beach area of Florida. Where is it
} } currently located? I would highly recommend sending someone to pack
} } it properly. How is it going to be shipped? If it's going by truck,
} } it must be "padded van" unless you're going to have a lot more of it
} } disassembled, crated and put on pallets.
} }
} } With more info, I might be of more help.
} }
} } Ken Converse
} } owner
} }
} } QUALITY IMAGES
} } Servicing Scanning Electron Microscopes Since 1981
} } 474 So. Bridgton Rd.
} } Bridgton, ME 04009
} } 207-647-4348
} } Fax 207-647-2688
} } kenconverse-at-qualityimages.biz
} } qualityimages.biz
} }
} }
} }
} } -----Original Message-----
} } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
} } Sent: Friday, March 16, 2007 6:43 PM
} } To: kenconverse-at-qualityimages.biz
} } Subject: [Microscopy] New SEM donated to our lab- Can you identify the
} } accessory?
} }
} }
} }
} }
} }
} } ----------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ---------------------------------------------------------------------------
-
} }
} } Hello all,
} }
} } We have a new SEM on the way to us, it's a JEOL JSM-840. All I have
} } at this point are a couple of pictures, and I am a little flummoxed
} } about the accessories that are added on this instrument. I recognize
} } the secondary electron detector, and I believe that there is a
} } backscatter detector on it, but I'm not sure what the other
} } accessories are. Also, the photos that I have seen of the basic 840s
} } don't include a box sitting on top of the column, but this one has it.
} } It's a silver-ish box with black sides, and is barely visible in one
} } of the photos, but it is there, at a slight angle. (Which leads me to
} } the next question- how should it be secured for
} } shipping?)
} }
} } The whole microscope is being packed by people who don't necessarily
} } know how to package it. Do any of you have specific suggestions of
} } what they could do to prevent damage in shipping?
} }
} } Here is the URL to the photos of the microscope (Hosted on my personal
} } page which has not been updated in a long time...)
} } http://www.jkraft.net/JEOL/
} }
} } Thanks,
} }
} } Justin A. Kraft
} } Director
} } Center for Inquiry-Based Science Education
} }
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} } 28, 29 -- Subject: RE: [Microscopy] New SEM donated to our lab- Can you
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} Can you identify the accessory?
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From: kraftpiano-at-gmail.com
Date: Tue, 20 Mar 2007 15:55:28 -0500
Subject: [Microscopy] Re: New SEM donated to our lab- Moving a microscope -For Fun

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I was very amused by that quip as well. Of course, I was also amused
that they kept calling it an "Electron Scanner Microscope."

Oh, well.

Thanks for all of the replies, guys. Unfortunately, I have little to
no control over the packing. They will take basic suggestions, and do
some basic things, but generally they are in a different line of
business- electronics recycling. They were put in the position of
having it and not knowing how to sell it, so they ended up giving it
to me. The way I figure, I'll have them batten it down as best as
they are willing to do, and hope. (Logically, since according to them
it was taken out some 50 miles or so away from where they are, and
they took it out, I figure that any damage that will be done to it has
already been done.)

They're splitting the shipping costs with us as well, so the total out
of pocket expense for us is $300.

Worst case scenario I end up with a bunch of broken parts, but the
basis for putting together a working instrument over the next several
years. My degree is in physics, and so I will at least enjoy the
challenge of figuring it out from a circuits and wires point of view.

They don't have any manuals or anything- all I'm getting is the
column, the display console, and the power supply box. They sold the
pump box (Probably because that was something they recognized) on
Ebay, so my first challenge will be getting a pump up and running. I
might steal the one off of the ISI SX-40A I have at home for a little
while. After that, there is an air conditioning service vacuum sales
representative who is willing to donate one of his pumps and some
gauges and such.

When the SEM gets here, I'll post regular updates (If you're
interested) on our progress. One of my fortes is documentation, so I
will at least document the whole process on a website.

Doom and gloom predictions aside now, let's all hold our breaths and
cross our collective fingers and toes.

--Justin.

On 3/20/07, walck-at-southbaytech.com {walck-at-southbaytech.com} wrote:
}
}
}
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}
} You all have to hire the guys that moved the microscope on the TV show,
} "Crossing Jordan".
}
} In the story line, they had moved the EM out of the lab for cost cutting and
} had it in the basement. When they needed it, the boss told them to "go get
} my microscope" and they had it up and running in less than an hour.
}
} Well, I thought it was funny!
}
}
} -Scott
}
} Scott D. Walck, Ph.D.
} Technical Director
} South Bay Technology, Inc.
} 1120 Via Callejon
} San Clemente, CA 92673
}
} US Toll Free: 1-800-728-2233
} Tel: (949) 492-2600
} Fax: (949) 492-1499
}
} www.southbaytech.com
} walck-at-southbaytech.com
}
} -----Original Message-----
} X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
} Sent: Tuesday, March 20, 2007 11:06 AM
} To: Walck-at-SouthBayTech.com
} Subject: [Microscopy] Re: New SEM donated to our lab- Can you identify
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Ken,
}
} You make a VERY IMPORTANT point here: "Getting the instrument is the easy
} part. Getting it up and running consistently well takes a lot of work."
}
} In my SEM course, I always take time to point out to my students that they
} may be in a situation where someone wants to donate an EM to their school.
} No matter how well it is working when the donation is accepted, there is no
} guarantee that it will ever work properly in the new location unless
} professionals are involved in the packing, shipping and installation. I
} always recommend having $5-10K available. Hopefully, it will never be
} needed. My analogy is: "It's like having a baby. The easy part is producing
} the baby. Caring for it is a labor (hopefully of love)."
}
} JB
}
}
} } Justin,
} } Unless they are paying for the shipping, you're going to be out a
} } sizeable chunk of change just in the shipping costs. If someone
} } doesn't spend at least a few hours properly packing the system for
} } however it is going to be shipped (and how it will be shipped makes a
} } difference in how it is packed), you will receive a large anchor for
} } your boat. Unless you have someone at your end who is experienced and
} } willing to donate their time for getting this system back up and
} } running, you probably don't have the budget to get it running if you don't
} have the budget to have it packed properly.
} }
} } I hate to be so down on this because I currently support an SEM at a
} } high school because I firmly believe that having the students have the
} } opportunity to play with the toys that we get to play with in the real
} } world is a great way to inspire them to get the education they need.
} } However, in addition to not seeing some major components to the system
} } in your photos (you also need someone to evalute what's there), I can
} } tell you that I once packed a system for a customer on a tight budget.
} } I packed it for shipment by padded van (which is a much less intensive
} } packing job than for a basic truck), but they decided to just put it on
} } an Estes 18 wheeler despite what I had told them. By the time it had gone
} from PA to CA it was trashed.
} } Penny wise and pound foolish. And they had PAID for that system!
} }
} } Unfortunately aquiring a free SEM is an expensive affair unless you can
} } find someone to do it all properly because THEY are also committed to
} } the students. It's a sizeable commitment to take on even one SEM
} } pro-bono, but maybe someone in your area could contact you, if they're
} interested.
} } Personally, I would like to see the manufacturers take on a high school
} } system pro-bono for every x number of field service engineers, but that
} } probably won't ever happen. (Are you guys paying attention, here?).
} }
} } I sincerely hope that someone DOES contact you and I also hope that my
} } doom and gloom is off the mark.
} }
} } Best wishes
} } Ken Converse
} } Owner
} }
} } QUALITY IMAGES
} } Servicing Scanning Electron Microscopes Since 1981
} } 474 So. Bridgton Rd.
} } Bridgton, ME 04009
} } 207-647-4348
} } Fax 207-647-2688
} } kenconverse-at-qualityimages.biz
} } qualityimages.biz
} }
} }
} }
} } -----Original Message-----
} } X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com]
} } Sent: Saturday, March 17, 2007 2:28 PM
} } To: kenconverse-at-qualityimages.biz
} } Subject: Re: [Microscopy] RE: New SEM donated to our lab- Can you
} } identify the accessory?
} }
} }
} } It's in New Jersey right now, and unfortunately we don't have the
} } budget to send someone up there to pack it properly. The company who
} } is donating it will not spend more than a few minutes prepping it (They
} } aren't making money off of it, and they are electronics recyclers, so
} } they just see it as a pile of metal) so I need to make instructions as
} } simple and clear as possible, without having too much for them to do,
} } or they may just decide to Ebay the whole thing.
} }
} } --Justin.
} }
} } On 3/17/07, kenconverse-at-qualityimages.biz
} } {kenconverse-at-qualityimages.biz}
} } wrote:
} } }
} } }
} } }
} } }
} } } ---------------------------------------------------------------------
} } } -
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} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } } America
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} } }
} } -----------------------------------------------------------------------
} } -----
} } }
} } } Justin,
} } } I believe what you have is a JXA-840 (microprobe) with 2 wavelength
} } } spectrometers and a light microscope for establishing the proper WD
} } } for using those spectrometers. The Box on top is an ion pump so that
} } } the system can be used with a LaB6 cathode, if you choose (and have
} } } received the correct wehnelt cap).
} } }
} } } Whether it is an 840 or 840A could be told by a look at the
} } } electronics console, but you don't have any pictures of that or the
} } } electronics rack that should be present for the spectometers. I do
} } } see the power supply console in a couple of pictures. Does it
} } } include the rotary pump and possibly compressor?
} } }
} } } As far as prepping for shipping, the first thing that jumps out at
} } } me is that the table isn't bolted down! At a minimum you need to
} } } get 4 M16x2.00x40mm bolts and 16mm washers to secure the table. You
} } } also need to remove the magnet from the ion pump. Actually, most of
} } } the column should be removed and packed separately unless you're
} } } planning to move it only a few miles at very low speed. I also
} } } suspect that the WDS units and light microscope should be removed.
} } } All 3 are complex and fragile.
} } }
} } } Is it diffusion pumped or turbo pumped? If it's turbo pumped, the
} } } pump must be removed before shipping. Also the high voltage oil tank
} } } in the electronics console must be removed and the circuit board on
} } } its side protected from damage.
} } }
} } } This is going to the W. Palm Beach area of Florida. Where is it
} } } currently located? I would highly recommend sending someone to pack
} } } it properly. How is it going to be shipped? If it's going by truck,
} } } it must be "padded van" unless you're going to have a lot more of it
} } } disassembled, crated and put on pallets.
} } }
} } } With more info, I might be of more help.
} } }
} } } Ken Converse
} } } owner
} } }
} } } QUALITY IMAGES
} } } Servicing Scanning Electron Microscopes Since 1981
} } } 474 So. Bridgton Rd.
} } } Bridgton, ME 04009
} } } 207-647-4348
} } } Fax 207-647-2688
} } } kenconverse-at-qualityimages.biz
} } } qualityimages.biz
} } }
} } }
} } }
} } } -----Original Message-----
} } } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
} } } Sent: Friday, March 16, 2007 6:43 PM
} } } To: kenconverse-at-qualityimages.biz
} } } Subject: [Microscopy] New SEM donated to our lab- Can you identify the
} } } accessory?
} } }
} } }
} } }
} } }
} } }
} } } ----------------------------------------------------------------------
} } } ------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
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} } }
} } ---------------------------------------------------------------------------
} -
} } }
} } } Hello all,
} } }
} } } We have a new SEM on the way to us, it's a JEOL JSM-840. All I have
} } } at this point are a couple of pictures, and I am a little flummoxed
} } } about the accessories that are added on this instrument. I recognize
} } } the secondary electron detector, and I believe that there is a
} } } backscatter detector on it, but I'm not sure what the other
} } } accessories are. Also, the photos that I have seen of the basic 840s
} } } don't include a box sitting on top of the column, but this one has it.
} } } It's a silver-ish box with black sides, and is barely visible in one
} } } of the photos, but it is there, at a slight angle. (Which leads me to
} } } the next question- how should it be secured for
} } } shipping?)
} } }
} } } The whole microscope is being packed by people who don't necessarily
} } } know how to package it. Do any of you have specific suggestions of
} } } what they could do to prevent damage in shipping?
} } }
} } } Here is the URL to the photos of the microscope (Hosted on my personal
} } } page which has not been updated in a long time...)
} } } http://www.jkraft.net/JEOL/
} } }
} } } Thanks,
} } }
} } } Justin A. Kraft
} } } Director
} } } Center for Inquiry-Based Science Education
} } }
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} } } 6, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com}
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} } } 6, 26 -- Subject: New SEM donated to our lab- Can you identify the
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} } } 28, 29 -- From kenconverse-at-qualityimages.biz Sat Mar 17 11:36:36 2007
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} } {Microscopy-at-MSA.Microscopy.Com}
} } } 28, 29 -- Subject: RE: [Microscopy] New SEM donated to our lab- Can you
} } identify the accessory?
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} } 19, 30 -- Subject: RE: [Microscopy] RE: New SEM donated to our lab-
} } Can you identify the accessory?
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From: gary-at-gaugler.com
Date: Tue, 20 Mar 2007 16:22:15 -0500
Subject: [Microscopy] New SEM donated to our lab- Moving a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


If the column has a turbo, they need to tape it
in the center position using duct tape. One
basically make an X arrangement using lots of
tape around the pump and side steel columns.
This has worked for me in the past several moves.
If they don't do this, you can kiss the bellows
goodbye.

If you can't get a good mech pump, let me know.
I have several lying around here that are not being
used. I have a Varian and some other brand
that are the same or greater capacity as Edwards
RV8. Worst case is shipping cost and potential
need to rebuild the pump vanes. Pump is free.
You can get rebuild kits from Dunniway Vacuum.

gary g.


At 12:57 PM 3/20/2007, you wrote:




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11, 21 -- microscope -For Fun
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From: kenconverse-at-qualityimages.biz
Date: Tue, 20 Mar 2007 17:05:59 -0500
Subject: [Microscopy] Re: New SEM donated to our lab- Moving a microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Justin,
I'm feeling better since you seem to have an idea of what you're up against.
Enough doom and gloom - I'm keeping my fingers crossed for you.

As to the pump: JEOL automatically vents the RP line upon shut-down and
normally also shuts off the RP. Their RPs (rotary pumps, same as MP -
mechanical pump) run on 200VAC. A 240VAC motor may complain terminally.
One alternative I have used is to build a relay box that uses their 200VAC
control signal but allows you to use a 120VAC Welch, Edwards, Alcatel (pick
your flavor) pump and not end up with the system shutting down and your pump
running continuously on a major leak. I can send you some info on that. I
suppose a buck/boost transformer might also do the trick.

It sound like you aren't getting the JEOL toolkit, which is too bad. If you
send me your snailmail address I will send you schematics. I will also
throw in a CD with both the user's manual and the service manual. This will
open on MS Word document reader. If you need Adobe Acrobat, let me know but
it will take a little longer, but I can do that for you.

We can make school fun!

Ken converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz



-----Original Message-----
X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
Sent: Tuesday, March 20, 2007 4:58 PM
To: kenconverse-at-qualityimages.biz

I was very amused by that quip as well. Of course, I was also amused that
they kept calling it an "Electron Scanner Microscope."

Oh, well.

Thanks for all of the replies, guys. Unfortunately, I have little to no
control over the packing. They will take basic suggestions, and do some
basic things, but generally they are in a different line of
business- electronics recycling. They were put in the position of having it
and not knowing how to sell it, so they ended up giving it to me. The way I
figure, I'll have them batten it down as best as they are willing to do, and
hope. (Logically, since according to them it was taken out some 50 miles or
so away from where they are, and they took it out, I figure that any damage
that will be done to it has already been done.)

They're splitting the shipping costs with us as well, so the total out of
pocket expense for us is $300.

Worst case scenario I end up with a bunch of broken parts, but the basis for
putting together a working instrument over the next several years. My
degree is in physics, and so I will at least enjoy the challenge of figuring
it out from a circuits and wires point of view.

They don't have any manuals or anything- all I'm getting is the column, the
display console, and the power supply box. They sold the pump box (Probably
because that was something they recognized) on Ebay, so my first challenge
will be getting a pump up and running. I might steal the one off of the ISI
SX-40A I have at home for a little while. After that, there is an air
conditioning service vacuum sales representative who is willing to donate
one of his pumps and some gauges and such.

When the SEM gets here, I'll post regular updates (If you're
interested) on our progress. One of my fortes is documentation, so I will
at least document the whole process on a website.

Doom and gloom predictions aside now, let's all hold our breaths and cross
our collective fingers and toes.

--Justin.

On 3/20/07, walck-at-southbaytech.com {walck-at-southbaytech.com} wrote:
}
}
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} You all have to hire the guys that moved the microscope on the TV
} show, "Crossing Jordan".
}
} In the story line, they had moved the EM out of the lab for cost
} cutting and had it in the basement. When they needed it, the boss
} told them to "go get my microscope" and they had it up and running in
} less than an hour.
}
} Well, I thought it was funny!
}
}
} -Scott
}
} Scott D. Walck, Ph.D.
} Technical Director
} South Bay Technology, Inc.
} 1120 Via Callejon
} San Clemente, CA 92673
}
} US Toll Free: 1-800-728-2233
} Tel: (949) 492-2600
} Fax: (949) 492-1499
}
} www.southbaytech.com
} walck-at-southbaytech.com
}
} -----Original Message-----
} X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
} Sent: Tuesday, March 20, 2007 11:06 AM
} To: Walck-at-SouthBayTech.com
} Subject: [Microscopy] Re: New SEM donated to our lab- Can you identify
}
}
}
}
} ----------------------------------------------------------------------
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}
} Ken,
}
} You make a VERY IMPORTANT point here: "Getting the instrument is the
} easy part. Getting it up and running consistently well takes a lot of
} work."
}
} In my SEM course, I always take time to point out to my students that
} they may be in a situation where someone wants to donate an EM to
} their school. No matter how well it is working when the donation is
} accepted, there is no guarantee that it will ever work properly in the
} new location unless professionals are involved in the packing,
} shipping and installation. I always recommend having $5-10K available.
} Hopefully, it will never be needed. My analogy is: "It's like having a
} baby. The easy part is producing the baby. Caring for it is a labor
} (hopefully of love)."
}
} JB
}
}
} } Justin,
} } Unless they are paying for the shipping, you're going to be out a
} } sizeable chunk of change just in the shipping costs. If someone
} } doesn't spend at least a few hours properly packing the system for
} } however it is going to be shipped (and how it will be shipped makes a
} } difference in how it is packed), you will receive a large anchor for
} } your boat. Unless you have someone at your end who is experienced
} } and willing to donate their time for getting this system back up and
} } running, you probably don't have the budget to get it running if you
} } don't
} have the budget to have it packed properly.
} }
} } I hate to be so down on this because I currently support an SEM at a
} } high school because I firmly believe that having the students have
} } the opportunity to play with the toys that we get to play with in the
} } real world is a great way to inspire them to get the education they
} } need. However, in addition to not seeing some major components to the
} } system in your photos (you also need someone to evalute what's
} } there), I can tell you that I once packed a system for a customer on
} } a tight budget. I packed it for shipment by padded van (which is a
} } much less intensive packing job than for a basic truck), but they
} } decided to just put it on an Estes 18 wheeler despite what I had told
} } them. By the time it had gone
} from PA to CA it was trashed.
} } Penny wise and pound foolish. And they had PAID for that system!
} }
} } Unfortunately aquiring a free SEM is an expensive affair unless you
} } can find someone to do it all properly because THEY are also
} } committed to the students. It's a sizeable commitment to take on
} } even one SEM pro-bono, but maybe someone in your area could contact
} } you, if they're
} interested.
} } Personally, I would like to see the manufacturers take on a high
} } school system pro-bono for every x number of field service engineers,
} } but that probably won't ever happen. (Are you guys paying attention,
} } here?).
} }
} } I sincerely hope that someone DOES contact you and I also hope that
} } my doom and gloom is off the mark.
} }
} } Best wishes
} } Ken Converse
} } Owner
} }
} } QUALITY IMAGES
} } Servicing Scanning Electron Microscopes Since 1981
} } 474 So. Bridgton Rd.
} } Bridgton, ME 04009
} } 207-647-4348
} } Fax 207-647-2688
} } kenconverse-at-qualityimages.biz
} } qualityimages.biz
} }
} }
} }
} } -----Original Message-----
} } X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com]
} } Sent: Saturday, March 17, 2007 2:28 PM
} } To: kenconverse-at-qualityimages.biz
} } Subject: Re: [Microscopy] RE: New SEM donated to our lab- Can you
} } identify the accessory?
} }
} }
} } It's in New Jersey right now, and unfortunately we don't have the
} } budget to send someone up there to pack it properly. The company who
} } is donating it will not spend more than a few minutes prepping it
} } (They aren't making money off of it, and they are electronics
} } recyclers, so they just see it as a pile of metal) so I need to make
} } instructions as simple and clear as possible, without having too much
} } for them to do, or they may just decide to Ebay the whole thing.
} }
} } --Justin.
} }
} } On 3/17/07, kenconverse-at-qualityimages.biz
} } {kenconverse-at-qualityimages.biz}
} } wrote:
} } }
} } }
} } }
} } }
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} } } America
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From: paul-at-biochem.bumc.bu.edu
Date: Tue, 20 Mar 2007 18:52:56 -0500
Subject: [Microscopy] AskAMicroscopist: Plastic Embedment of Mouse Lens & Eye for TEM

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Email: paul-at-biochem.bumc.bu.edu
Name: Paul Toselli

Organization: Boston University Medical School

Education: Graduate College

Location: Boston, MA

Title: Plastic Embedment of Mouse Lens & Eye for TEM

Question: I would like a method to fix and Araldite/DDSA-embed a mouse eye for examination under a transmission electron microscope. The mouse would be approximately one-month of age. Would you please help me? Thank you.

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==============================Original Headers==============================
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8, 12 -- Subject: AskAMicroscopist: Plastic Embedment of Mouse Lens & Eye for TEM
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From: charlesping-at-hotmail.com
Date: Wed, 21 Mar 2007 05:53:56 -0500
Subject: [Microscopy] JEOL 6400F SEM need help for the interface

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, all

Thank you all for your responses.

The new Auger Spectrometer is a newly designed one and we just want to test
it to get some obvious Auger peaks, like the carbon or oxide. And the
availability of Auger Spectrometer in a SEM vacuum is one purpose of this
design as well. I know it is hard to work in that vacuum pressure, but we
do provide some methods to clean the sample in situ.

Cheers

Charles

} From: colijn.1-at-osu.edu
} Reply-To: colijn.1-at-osu.edu
} To: charlesping-at-hotmail.com
} Subject: [Microscopy] Re: JEOL 6400F SEM need help for the interface
} Date: Tue, 20 Mar 2007 08:56:02 -0500
}
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From: johnf-at-geology.wisc.edu
Date: Wed, 21 Mar 2007 10:27:55 -0500
Subject: [Microscopy] HKL EBSD with Hitachi SEMs

Contents Retrieved from Microscopy Listserver Archives
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Could folks with HKL EBSD systems with Hitachi SEMs please contact me
directly (johnf-at-geology.wisc.edu). I am particularly interested in
which version of the KE bse amplifier they were supplied with.
Apparently there are different KE versions built for different sems.
We are continuing to try to determine the source of 200 KHZ noise on
bse images coming from that amplifier.

John
--
========================================================
John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480
Cameron Electron Microprobe Lab lab: (608) 265-4798
Dept of Geology & Geophysics fax: (608) 262-0693
University of Wisconsin home: (608) 274-2245
1215 West Dayton St. email: johnf-at-geology.wisc.edu
Madison, WI 53706 amateur radio: WA3BTA
Personal http://www.geology.wisc.edu/~johnf/
Probe lab http://www.geology.wisc.edu/~johnf/sx51.html
Probe Sign Up Calender:
http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi

"The first rule of all intelligent tinkering is to save every cog and
wheel." -- Aldo Leopold

"For a successful technology, reality must take precedence over
public relations, for Nature cannot be fooled." -- Richard P.
Feynman

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From: kraftpiano-at-gmail.com
Date: Wed, 21 Mar 2007 14:01:09 -0500
Subject: [Microscopy] AAArgh: SEM Donation gone horribly awry! Cany anyone help?!?!?

Contents Retrieved from Microscopy Listserver Archives
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Ok. The company has contacted me and told me that I need to pay more
money to have the microscope packaged, otherwise they will just strap
it to the pallets and ship it out. Is there anybody in or around
Brockport, NY who wouldn't mind going over and taking the ion pump off
the top of the instrument? They refuse to do it unless I pay extra.
("I can only guess that it will take three to four hours for someone
to do that")

The ion pump is hanging at a strange angle, and I'm worried that it
will do major damage if left on.

Help!

--Justin A. Kraft
Director
Center for Inquiry-Based Science Education

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4, 26 -- Date: Wed, 21 Mar 2007 15:01:07 -0400
4, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com}
4, 26 -- To: microscopy-at-microscopy.com
4, 26 -- Subject: AAArgh: SEM Donation gone horribly awry! Cany anyone help?!?!?
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From: kraftpiano-at-gmail.com
Date: Wed, 21 Mar 2007 16:18:30 -0500
Subject: [Microscopy] Update: Microscope saved by savvy straight-talk.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I just got off of a lengthy phone conversation with the gentleman at
the warehouse who is shipping out our new SEM. He was insisting that
he needed more money to remove the magnets from the ion pump, since he
didn't even know what it was.

He walked out to the instrument in his warehouse, and I explained it
to him, at which point he removed the magnets personally since THERE
WERE NO SCREWS HOLDING THEM IN!

After which he replied "Was that it?"

I thanked him, and our microscope will be beginning its journey on Friday.

Everyone can breathe a sigh of relief for a moment, but keep the
fingers crossed about the WDS crystals...

--Justin A. Kraft

==============================Original Headers==============================
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6, 26 -- Date: Wed, 21 Mar 2007 17:18:20 -0400
6, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com}
6, 26 -- To: microscopy-at-microscopy.com
6, 26 -- Subject: Update: Microscope saved by savvy straight-talk.
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From: zhang.zaoli-at-google.com
Date: Wed, 21 Mar 2007 17:42:46 -0500
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: From PEELS in Tia to

Contents Retrieved from Microscopy Listserver Archives
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Email: zhang.zaoli-at-google.com
Name: zaoli zhang

Organization: Ulm university, electron microscopy center

Title-Subject: [Filtered] From PEELS in Tia to EELS in Digital Micrograph

Question: Dear Colleagues

I acquired EELS spectra in STEM mode using TIA software, I have no idea on how to transfer EELS spectra in TIA(STEM) to Digital Micrograph for further processing, Does anyone know how to do that ?

thanks in advance ,


Zaoli Zhang




---------------------------------------------------------------------------

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13, 12 -- From: zhang.zaoli-at-google.com (by way of MicroscopyListserver)
13, 12 -- Subject: [Filtered] MicroscopyListserverviaWWW: From PEELS in Tia to
13, 12 -- EELS in Digital Micrograph
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==============================End of - Headers==============================




From: rosenj03-at-med.nyu.edu
Date: Wed, 21 Mar 2007 17:43:34 -0500
Subject: [Microscopy] viaWWW: Sorval MT2 and MT5000 microtomes

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Email: rosenj03-at-med.nyu.edu
Name: J. Rosenbluth

Organization: NYU School of Med.

Title-Subject: [Filtered] TEM

Question: Looking for someone to service Sorval MT2 and MT5000 microtomes in New York City (Manhattan).

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Wed Mar 21 17:43:34 2007
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6, 11 -- From: rosenj03-at-med.nyu.edu (by way of MicroscopyListserver)
6, 11 -- Subject: viaWWW: Sorval MT2 and MT5000 microtomes
6, 11 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: RCsencsits-at-lbl.gov
Date: Wed, 21 Mar 2007 18:09:21 -0500
Subject: [Microscopy] Re: [Filtered] MicroscopyListserverviaWWW: From PEELS in Tia to

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

FEI should supply that information or insist that you want to acquire
data with digitalMicrosgraph!!

Customer demands move company hands and heads!

Roseann Csencsits


On Mar 21, 2007, at 3:49 PM, zhang.zaoli-at-google.com wrote:

}
}
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} using the WWW based Form at http://microscopy.com/
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} replying
} please copy both zhang.zaoli-at-google.com as well as the
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}
} Email: zhang.zaoli-at-google.com
} Name: zaoli zhang
}
} Organization: Ulm university, electron microscopy center
}
} Title-Subject: [Filtered] From PEELS in Tia to EELS in Digital
} Micrograph
}
} Question: Dear Colleagues
}
} I acquired EELS spectra in STEM mode using TIA software, I have no
} idea on how to transfer EELS spectra in TIA(STEM) to Digital
} Micrograph for further processing, Does anyone know how to do that ?
}
} thanks in advance ,
}
}
} Zaoli Zhang
}
}
}
}
} ----------------------------------------------------------------------
} -----
}
} ==============================Original
} Headers==============================
} 13, 12 -- From zaluzec-at-microscopy.com Wed Mar 21 17:42:46 2007
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} ESMTP id l2LMgjmu015635
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From: lovett-at-tcnj.edu
Date: Thu, 22 Mar 2007 07:34:34 -0500
Subject: [Microscopy] Re: viaWWW: Sorval MT2 and MT5000 microtomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Question: Looking for someone to service Sorval MT2 and MT5000
microtomes in New York City (Manhattan).

We have been very satisfied with:

MOC (Microscopical Optical Consulting, Inc)
P.O. Box 586
Valley Cottage, NY 10989

MOCLeica.aol.com

Phone: either 845-268-6450 or 914-268-6450
(I have two cards, and I think the 845 area code is the more recent

Excellent service, good pricing

Don


--
_____________________________________________________________
Donald L. Lovett E-mail: Lovett-at-tcnj.edu
Professor Phone: 609-771-2876
Department of Biology Fax: 609-637-5118
The College of New Jersey
P.O. Box 7718
Ewing, NJ 08628-0718


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From: mcauliff-at-umdnj.edu
Date: Thu, 22 Mar 2007 09:08:11 -0500
Subject: [Microscopy] Re: AskAMicroscopist: Plastic Embedment of Mouse Lens &

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings!

Do you really want to fix and embed the whole eye?
Even assuming you could cut wrinkle-free thin sections of such a large
block I don't think the section would fit on a grid. AND, with such a
large section, you would never find what you were looking for in the EM.
I suggest defining your research target more precisely. Also consider
colaborating with an anatomist/morphologist skilled in EM.
If you want to look at the lens you should know that the lens is very
difficult to infiltrate so Araddite/DDSA may not be the best choice of
epoxy.
Go the to library and get a good textbook of ophthamology (or even your
old histology text from medical school). The references will tell you
what those who have already attacked this problem have done as far as
fixation, embedding, etc. Why re-invent the wheel?

Geoff

paul-at-biochem.bumc.bu.edu wrote:

} ----------------------------------------------------------------------------
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--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



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9, 36 -- Subject: Re: [Microscopy] AskAMicroscopist: Plastic Embedment of Mouse Lens &
9, 36 -- Eye for TEM
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From: MSHERWOOD-at-PARTNERS.ORG
Date: Thu, 22 Mar 2007 09:15:11 -0500
Subject: [Microscopy] viaWWW: Sorval MT2 and MT5000 microtomes

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To: 'rosenj03-at-med.nyu.edu'

This Question/Comment was submitted to the Microscopy Listserver
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Email: rosenj03-at-med.nyu.edu
Name: J. Rosenbluth

Organization: NYU School of Med.

Title-Subject: [Filtered] TEM

Question: Looking for someone to service Sorval MT2 and MT5000 microtomes in New
York City (Manhattan).

---------------------------------------------------------------------------

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From: a.d.mckinnon-at-abdn.ac.uk
Date: Thu, 22 Mar 2007 10:41:18 -0500
Subject: [Microscopy] Re: AskAMicroscopist: Plastic Embedment of Mouse

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Paul,

Whilst this is good advice from Geoff, fixing and processing the whole
eye has its advantages in minimising disruption to the internal
structures, and in particular the retinal layers.

Whole eye however, is extremely difficult to infiltrate and the great
variation in structure density makes this a challenging proposition.

The following procedure improved our LM sectioning results, and I would
suggest giving it a try for EM processing.

Following initial fixation at 4'C in 2.5% glut, trim a superficial slice
off one side of the eye - just enough to expose the inner cavity. We use
indented bluetack to hold the eye in position whilst slicing with a
razor blade in a line parallel to the plane of the optic nerve which is
pointing down into the bluetack.

Don't be tempted to go for a second parallel slice as this causes a lot
of disruption to the internal structures, and return the eye to fix for
a further overnight period at 4'C. Follow this with a prolonged
processing schedule using a low viscosity epoxy resin.

If (safe) Spurr is no longer available you could try TAAB's low
viscosity epoxy resin (www.taab.co.uk). We have already tested this
product as an alternative to Spurr for when our supplies run out. It is
slightly more viscous than Spurr but readily available.

I would then suggest doing a final dissection to remove the lens (if not
required) and to obtain blocks that are of a reasonable size for
ultrathin sectioning and to include your area of interest. If this
includes the retinal layer, it would probably be useful to include a bit
of the optic nerve as an identifier. I would then suggest returning the
samples to fresh resin for a final infiltration with pure resin (under
vacuum at 50'C) for an hour or so, prior to transferring to embedding
moulds and polymerising at 60'C for 24-48hrs. Flat embedding will
probably work best if it is a cross section you are going for.

Contact me direct if you want to follow our prolonged epoxy resin
schedule.

Hope this helps.

Alastair

Alastair McKinnon
Histology & EM Facility Manager
University of Aberdeen, Institute of Medical Science
Foresterhill, Aberdeen, AB25 2ZD
tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab)
fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk
www.abdn.ac.uk/ims/h-em
-----Original Message-----
X-from: mcauliff-at-umdnj.edu [mailto:mcauliff-at-umdnj.edu]
Sent: 22 March 2007 14:14
To: Mckinnon, Alastair D.

Greetings!

Do you really want to fix and embed the whole eye?
Even assuming you could cut wrinkle-free thin sections of such a large
block I don't think the section would fit on a grid. AND, with such a
large section, you would never find what you were looking for in the EM.
I suggest defining your research target more precisely. Also consider
colaborating with an anatomist/morphologist skilled in EM.
If you want to look at the lens you should know that the lens is very
difficult to infiltrate so Araddite/DDSA may not be the best choice of
epoxy.
Go the to library and get a good textbook of ophthamology (or even your
old histology text from medical school). The references will tell you
what those who have already attacked this problem have done as far as
fixation, embedding, etc. Why re-invent the wheel?

Geoff

paul-at-biochem.bumc.bu.edu wrote:

} -----------------------------------------------------------------------
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--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



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Mar 2007 10:09:20 -0400 9, 36 -- From: Geoff McAuliffe
{mcauliff-at-umdnj.edu} 9, 36 -- Subject: Re: [Microscopy]
AskAMicroscopist: Plastic Embedment of Mouse Lens & 9, 36 -- Eye for
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From: gary-at-gaugler.com
Date: Thu, 22 Mar 2007 13:11:00 -0500
Subject: [Microscopy] FIB-SIMS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi listers:

There are references to FIB-SIMS I have found
but so far, no specifics about what SIMS.

Does anyone know who makes a SIMS that might
fit on a FEI Nova 600 FIB? Main injector is Ga.

tnx,
gary g.


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From: rosenj03-at-med.nyu.edu
Date: Thu, 22 Mar 2007 17:23:55 -0500
Subject: [Microscopy] viaWWW: TEM Service

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Email: rosenj03-at-med.nyu.edu
Name: J. Rosenbluth

Organization: NYU School of Medicine

Title-Subject: [Filtered] TEM

Question: Would appreciate suggestions re organizations competent to service:
1. JEOL CX100
2. Philips EM300


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From: ani.issaian-at-csun.edu
Date: Thu, 22 Mar 2007 17:46:53 -0500
Subject: [Microscopy] Re: viaWWW: Sorval MT2 and MT5000

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Does any one know of service for the same microtomes and a LKB Bromma 8300
in the Los Angeles area? Thanks,


Ani
}
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From: Tanya.Hayes-at-northampton.ac.uk
Date: Fri, 23 Mar 2007 07:43:16 -0500
Subject: [Microscopy] viaWWW: SEM: lines on slow scan/capture

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Email: Tanya.Hayes-at-northampton.ac.uk
Name: Tanya Hayes

Organization: The University of Northampton

Title-Subject: [Filtered] SEM: lines on slow scan/capture

Question: We have an Hitachi S3000N SEM. We have intermittent lines appearing across our images, mainly sourcing in bands from brighter areas, all the way across the image. This has got much worse over the last few weeks. We have ruled out electrical interference & vibrations. Has anyone got any ideas?

Tanya Hayes
British School of Leather Technology
The University of Northampton
Northampton
United Kingdom


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From: raristau-at-ims.uconn.edu
Date: Fri, 23 Mar 2007 08:57:06 -0500
Subject: [Microscopy] Re: New SEM donated to our lab- Moving a microscope -For Fun

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Tanya

if you have ruled out simple charging effects then maybe you have a
similar fault to us.

We have a similar intermittent problem on our S3000N which is associated
with fluctuation in the filament emission readout. It doesn't seem to be
specific to either high pressure or high vacuum mode but sometimes will
stabilise if run at a higher voltage and dropped back down - makes it
seem like minor contamination. Generally this "flickering" effect gets
progressively worse with the age of the filament (but is it an effect of
age or is the flickering affecting the life of the filament?).

The trouble is the effect could be self-fulfilling because the shortened
filament life increases gun contamination and so may help to induce the
effect.

The erratic nature of the problem has made it difficult to solve or
indeed know if it has really gone away. I had hoped that it might either
go away or deteriorate to a point where the cause could be found.

Sorry - no real answers and I can't be sure from your description if we
even have a similar fault. But I will be interested in any progress you
make or suggestions from the readership.

Oh one thing that seems to make the problem worse (although may not be
the only cause) is that the top of the Wenhelt cap can slowly work a
little loose and although it may not be the only cause just gently
nipping it tight can help.

Good luck and if there's anything else that you need help with I'm sure
I can be equally as informative (or not). Please contact me directly by
email if you want a phone number.

Malcolm

Malcolm Haswell
e.m. unit
Chemispec
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: Tanya.Hayes-at-northampton.ac.uk

Just in case the enclosed link is unknown to the listserver, I am sending it
along on this thread. I think anyone who is attempting to reassemble an old
EM should get a smile and perhaps inspiration from the sincere efforts of
this fellow:
http://www.home.neab.net/gandalf/EM-lab/TEM100CX/

I would also be interested in learning if anyone has found similar diary's
on the web that could be shared with the listserver.

Cheers

Roger A. Ristau, PhD
Electron Microscopy Specialist
Institute of Materials Science
97 North Eagleville Road
University of Connecticut
Storrs, CT 06269
vox: 860-486-5379
fax: 860-486-4745



} From: kraftpiano-at-gmail.com
} Reply-To: kraftpiano-at-gmail.com
} Date: Tue, 20 Mar 2007 16:00:13 -0500
} To: raristau-at-ims.uconn.edu
} Subject: [Microscopy] Re: New SEM donated to our lab- Moving a microscope -For
} Fun
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I was very amused by that quip as well. Of course, I was also amused
} that they kept calling it an "Electron Scanner Microscope."
}
} Oh, well.
}
} Thanks for all of the replies, guys. Unfortunately, I have little to
} no control over the packing. They will take basic suggestions, and do
} some basic things, but generally they are in a different line of
} business- electronics recycling. They were put in the position of
} having it and not knowing how to sell it, so they ended up giving it
} to me. The way I figure, I'll have them batten it down as best as
} they are willing to do, and hope. (Logically, since according to them
} it was taken out some 50 miles or so away from where they are, and
} they took it out, I figure that any damage that will be done to it has
} already been done.)
}
} They're splitting the shipping costs with us as well, so the total out
} of pocket expense for us is $300.
}
} Worst case scenario I end up with a bunch of broken parts, but the
} basis for putting together a working instrument over the next several
} years. My degree is in physics, and so I will at least enjoy the
} challenge of figuring it out from a circuits and wires point of view.
}
} They don't have any manuals or anything- all I'm getting is the
} column, the display console, and the power supply box. They sold the
} pump box (Probably because that was something they recognized) on
} Ebay, so my first challenge will be getting a pump up and running. I
} might steal the one off of the ISI SX-40A I have at home for a little
} while. After that, there is an air conditioning service vacuum sales
} representative who is willing to donate one of his pumps and some
} gauges and such.
}
} When the SEM gets here, I'll post regular updates (If you're
} interested) on our progress. One of my fortes is documentation, so I
} will at least document the whole process on a website.
}
} Doom and gloom predictions aside now, let's all hold our breaths and
} cross our collective fingers and toes.
}
} --Justin.
}
} On 3/20/07, walck-at-southbaytech.com {walck-at-southbaytech.com} wrote:
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
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} } ----------------------------------------------------------------------------
} }
} } You all have to hire the guys that moved the microscope on the TV show,
} } "Crossing Jordan".
} }
} } In the story line, they had moved the EM out of the lab for cost cutting and
} } had it in the basement. When they needed it, the boss told them to "go get
} } my microscope" and they had it up and running in less than an hour.
} }
} } Well, I thought it was funny!
} }
} }
} } -Scott
} }
} } Scott D. Walck, Ph.D.
} } Technical Director
} } South Bay Technology, Inc.
} } 1120 Via Callejon
} } San Clemente, CA 92673
} }
} } US Toll Free: 1-800-728-2233
} } Tel: (949) 492-2600
} } Fax: (949) 492-1499
} }
} } www.southbaytech.com
} } walck-at-southbaytech.com
} }
} } -----Original Message-----
} } X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
} } Sent: Tuesday, March 20, 2007 11:06 AM
} } To: Walck-at-SouthBayTech.com
} } Subject: [Microscopy] Re: New SEM donated to our lab- Can you identify
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Ken,
} }
} } You make a VERY IMPORTANT point here: "Getting the instrument is the easy
} } part. Getting it up and running consistently well takes a lot of work."
} }
} } In my SEM course, I always take time to point out to my students that they
} } may be in a situation where someone wants to donate an EM to their school.
} } No matter how well it is working when the donation is accepted, there is no
} } guarantee that it will ever work properly in the new location unless
} } professionals are involved in the packing, shipping and installation. I
} } always recommend having $5-10K available. Hopefully, it will never be
} } needed. My analogy is: "It's like having a baby. The easy part is producing
} } the baby. Caring for it is a labor (hopefully of love)."
} }
} } JB
} }
} }
} } } Justin,
} } } Unless they are paying for the shipping, you're going to be out a
} } } sizeable chunk of change just in the shipping costs. If someone
} } } doesn't spend at least a few hours properly packing the system for
} } } however it is going to be shipped (and how it will be shipped makes a
} } } difference in how it is packed), you will receive a large anchor for
} } } your boat. Unless you have someone at your end who is experienced and
} } } willing to donate their time for getting this system back up and
} } } running, you probably don't have the budget to get it running if you don't
} } have the budget to have it packed properly.
} } }
} } } I hate to be so down on this because I currently support an SEM at a
} } } high school because I firmly believe that having the students have the
} } } opportunity to play with the toys that we get to play with in the real
} } } world is a great way to inspire them to get the education they need.
} } } However, in addition to not seeing some major components to the system
} } } in your photos (you also need someone to evalute what's there), I can
} } } tell you that I once packed a system for a customer on a tight budget.
} } } I packed it for shipment by padded van (which is a much less intensive
} } } packing job than for a basic truck), but they decided to just put it on
} } } an Estes 18 wheeler despite what I had told them. By the time it had gone
} } from PA to CA it was trashed.
} } } Penny wise and pound foolish. And they had PAID for that system!
} } }
} } } Unfortunately aquiring a free SEM is an expensive affair unless you can
} } } find someone to do it all properly because THEY are also committed to
} } } the students. It's a sizeable commitment to take on even one SEM
} } } pro-bono, but maybe someone in your area could contact you, if they're
} } interested.
} } } Personally, I would like to see the manufacturers take on a high school
} } } system pro-bono for every x number of field service engineers, but that
} } } probably won't ever happen. (Are you guys paying attention, here?).
} } }
} } } I sincerely hope that someone DOES contact you and I also hope that my
} } } doom and gloom is off the mark.
} } }
} } } Best wishes
} } } Ken Converse
} } } Owner
} } }
} } } QUALITY IMAGES
} } } Servicing Scanning Electron Microscopes Since 1981
} } } 474 So. Bridgton Rd.
} } } Bridgton, ME 04009
} } } 207-647-4348
} } } Fax 207-647-2688
} } } kenconverse-at-qualityimages.biz
} } } qualityimages.biz
} } }
} } }
} } }
} } } -----Original Message-----
} } } X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com]
} } } Sent: Saturday, March 17, 2007 2:28 PM
} } } To: kenconverse-at-qualityimages.biz
} } } Subject: Re: [Microscopy] RE: New SEM donated to our lab- Can you
} } } identify the accessory?
} } }
} } }
} } } It's in New Jersey right now, and unfortunately we don't have the
} } } budget to send someone up there to pack it properly. The company who
} } } is donating it will not spend more than a few minutes prepping it (They
} } } aren't making money off of it, and they are electronics recyclers, so
} } } they just see it as a pile of metal) so I need to make instructions as
} } } simple and clear as possible, without having too much for them to do,
} } } or they may just decide to Ebay the whole thing.
} } }
} } } --Justin.
} } }
} } } On 3/17/07, kenconverse-at-qualityimages.biz
} } } {kenconverse-at-qualityimages.biz}
} } } wrote:
} } } }
} } } }
} } } }
} } } }
} } } } ---------------------------------------------------------------------
} } } } -
} } } } ------
} } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } } } America
} } } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
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} } } }
} } } -----------------------------------------------------------------------
} } } -----
} } } }
} } } } Justin,
} } } } I believe what you have is a JXA-840 (microprobe) with 2 wavelength
} } } } spectrometers and a light microscope for establishing the proper WD
} } } } for using those spectrometers. The Box on top is an ion pump so that
} } } } the system can be used with a LaB6 cathode, if you choose (and have
} } } } received the correct wehnelt cap).
} } } }
} } } } Whether it is an 840 or 840A could be told by a look at the
} } } } electronics console, but you don't have any pictures of that or the
} } } } electronics rack that should be present for the spectometers. I do
} } } } see the power supply console in a couple of pictures. Does it
} } } } include the rotary pump and possibly compressor?
} } } }
} } } } As far as prepping for shipping, the first thing that jumps out at
} } } } me is that the table isn't bolted down! At a minimum you need to
} } } } get 4 M16x2.00x40mm bolts and 16mm washers to secure the table. You
} } } } also need to remove the magnet from the ion pump. Actually, most of
} } } } the column should be removed and packed separately unless you're
} } } } planning to move it only a few miles at very low speed. I also
} } } } suspect that the WDS units and light microscope should be removed.
} } } } All 3 are complex and fragile.
} } } }
} } } } Is it diffusion pumped or turbo pumped? If it's turbo pumped, the
} } } } pump must be removed before shipping. Also the high voltage oil tank
} } } } in the electronics console must be removed and the circuit board on
} } } } its side protected from damage.
} } } }
} } } } This is going to the W. Palm Beach area of Florida. Where is it
} } } } currently located? I would highly recommend sending someone to pack
} } } } it properly. How is it going to be shipped? If it's going by truck,
} } } } it must be "padded van" unless you're going to have a lot more of it
} } } } disassembled, crated and put on pallets.
} } } }
} } } } With more info, I might be of more help.
} } } }
} } } } Ken Converse
} } } } owner
} } } }
} } } } QUALITY IMAGES
} } } } Servicing Scanning Electron Microscopes Since 1981
} } } } 474 So. Bridgton Rd.
} } } } Bridgton, ME 04009
} } } } 207-647-4348
} } } } Fax 207-647-2688
} } } } kenconverse-at-qualityimages.biz
} } } } qualityimages.biz
} } } }
} } } }
} } } }
} } } } -----Original Message-----
} } } } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
} } } } Sent: Friday, March 16, 2007 6:43 PM
} } } } To: kenconverse-at-qualityimages.biz
} } } } Subject: [Microscopy] New SEM donated to our lab- Can you identify the
} } } } accessory?
} } } }
} } } }
} } } }
} } } }
} } } }
} } } } ----------------------------------------------------------------------
} } } } ------
} } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
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} } } }
} } } ---------------------------------------------------------------------------
} } -
} } } }
} } } } Hello all,
} } } }
} } } } We have a new SEM on the way to us, it's a JEOL JSM-840. All I have
} } } } at this point are a couple of pictures, and I am a little flummoxed
} } } } about the accessories that are added on this instrument. I recognize
} } } } the secondary electron detector, and I believe that there is a
} } } } backscatter detector on it, but I'm not sure what the other
} } } } accessories are. Also, the photos that I have seen of the basic 840s
} } } } don't include a box sitting on top of the column, but this one has it.
} } } } It's a silver-ish box with black sides, and is barely visible in one
} } } } of the photos, but it is there, at a slight angle. (Which leads me to
} } } } the next question- how should it be secured for
} } } } shipping?)
} } } }
} } } } The whole microscope is being packed by people who don't necessarily
} } } } know how to package it. Do any of you have specific suggestions of
} } } } what they could do to prevent damage in shipping?
} } } }
} } } } Here is the URL to the photos of the microscope (Hosted on my personal
} } } } page which has not been updated in a long time...)
} } } } http://www.jkraft.net/JEOL/
} } } }
} } } } Thanks,
} } } }
} } } } Justin A. Kraft
} } } } Director
} } } } Center for Inquiry-Based Science Education
} } } }
} } } } ==============================Original
} } } } Headers==============================
} } } } 6, 26 -- From kraftpiano-at-gmail.com Fri Mar 16 17:40:22 2007
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7, 17 -- From raristau-at-ims.uconn.edu Fri Mar 23 08:57:06 2007
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From: dac-at-research.umass.edu
Date: Fri, 23 Mar 2007 09:34:26 -0500
Subject: [Microscopy] Re: viaWWW: SEM: lines on slow scan/capture

Contents Retrieved from Microscopy Listserver Archives
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Hi Tanya,

Since you say it starts over bright objects, and is worse at slower scan
rates it sounds like charging to me.

A couple of suggestions. Have you changed to a different type of
specimen lately? Go back to an object you have successfully imaged
before, or one that should have no chance of charging issues - like a
calibration grating, or stick a copper TEM grid to a stub with silver or
graphite paint, even just the aluminum stub surface (rough up with
sandpaper to give a coarse texture), and see if it is still present. If
gone, it was charging and it could be something in the specimen type
(round objects or powders have contact/grounding issues and will be
worse) or something in the prep - maybe something in the sputtering?
Does the sample look reasonable - like it actually got sputtered? Note
that samples will look different for the same sputtered layer if smooth
vs. textured, white, etc.

If it is still present with samples that absolutely should not charge,
then it is in the instrument; is the stage grounding good?

I had bands all the way across, not just from prominent bright details,
and it was the spring contacts holding the filament - weak/oxidized -
causing fluctuations. If you can rule out the sample, it could be any
number of instrument related problems. But the "sourcing in bands from
brighter areas" - if I understand your meaning -sounds like charging.

Dale



Tanya.Hayes-at-northampton.ac.uk wrote:
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} Email: Tanya.Hayes-at-northampton.ac.uk
} Name: Tanya Hayes
}
} Organization: The University of Northampton
}
} Title-Subject: [Filtered] SEM: lines on slow scan/capture
}
} Question: We have an Hitachi S3000N SEM. We have intermittent lines appearing across our images, mainly sourcing in bands from brighter areas, all the way across the image. This has got much worse over the last few weeks. We have ruled out electrical interference & vibrations. Has anyone got any ideas?
}
} Tanya Hayes
} British School of Leather Technology
} The University of Northampton
} Northampton
} United Kingdom
}
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 7, 11 -- From zaluzec-at-microscopy.com Fri Mar 23 07:43:15 2007
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9, 22 -- From dac-at-research.umass.edu Fri Mar 23 09:34:25 2007
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From: mager-at-interchange.ubc.ca
Date: Fri, 23 Mar 2007 11:03:36 -0500
Subject: [Microscopy] viaWWW: SEM: lines on slow scan/capture

Contents Retrieved from Microscopy Listserver Archives
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Dear Tanya and other Hitachi S3000N owners,
I had exactly the same problem on my S3000N last year. Because it persisted
through filament and gun changes I knew it wasn't Wehnelt instability. I
checked the stage grounding, the BNC cap for the stage ground and used a
pure metal, conductive sample and still the problem was there. I checked the
objective and final aperture for dirt. I cleaned the column and nothing
seemed to help. I noticed the problem did not show up in the BSE imaging, so
then I looked at the secondary electron detector itself. When I removed it,
the cap over the fluorescent button was a bit loose and the button itself
was charging up. I tightened the cap and put a small dab of conductive paint
in the corner to make sure there was a path to ground for the electrons that
hit the button. I have not seen the problem since. The front of the button
has aluminum on it for grounding, but if there is a crack or not good
contact between the button and the rest of the SE detector, charge can build
up in the button itself, because it is made of a non-conductive plastic.
I hope this works for you.
Regards,

Mary Mager
Electron Microscopist
Materials Eng. UBC
#309 - 6350 Stores Road
Vancouver, B.C. V6T 1Z4
Canada
Tel: 604-822-5648
Fax: 604-822-3619
email: mager-at-interchange.ubc.ca

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Email: Tanya.Hayes-at-northampton.ac.uk
Name: Tanya Hayes

Organization: The University of Northampton

Title-Subject: [Filtered] SEM: lines on slow scan/capture

Question: We have an Hitachi S3000N SEM. We have intermittent lines
appearing across our images, mainly sourcing in bands from brighter areas,
all the way across the image. This has got much worse over the last few
weeks. We have ruled out electrical interference & vibrations. Has anyone
got any ideas?

Tanya Hayes
British School of Leather Technology
The University of Northampton
Northampton
United Kingdom


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From: jbpawley-at-wisc.edu
Date: Fri, 23 Mar 2007 13:03:42 -0500
Subject: [Microscopy] RE: viaWWW: SEM: lines on slow scan/capture/ Scintillator?

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Hi All

Well done Mary the give away here is the discharge line will have a little
dart on it when it is scintillator discharge. Sure you get white lines just
like charging but in this case the little dart is displayed on the lines.

Steve Chapman
Protrain for EM training & consultancy world wide
www.emcourses.com
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7711 606967

----- Original Message -----
X-from: {mager-at-interchange.ubc.ca}
To: {protrain-at-emcourses.com}
Sent: Friday, March 23, 2007 4:04 PM

I like Mary's solution.

I just wanted to mention a few things about the "button".

I assume that this Button is in fact the Scintillator that converts
the incoming accelerated SE into light that can then be conveyed to
and amplified by the PMT.

At one time the scintillators were made out of fluorescent plastic
but they had quite a short service lifetime (100 hrs) and only about
10 hours if used with high beam currents (nano-amps). The problem was
the immensely high radiation damage as the incoming SE signal, now
accelerated to about 10-15kV, smashed into the outer few microns of
the plastic.

Later, manufacturers switched to powdered-phosphor-deposited-on-glass
scintillators (usually P-47). This inorganic scintillator gave a much
longer service lifetime (1,000s of hours). However, as the powder was
an insulator, the deposited powder layer had to be covered with a
floated-on carbon or Formvar film and this was then coated with Al to
provide conductivity. (Don't put the Al directly onto the powder, it
keeps the light from getting out of the grains.). Sometimes the
glass-blank below was given a transparent "NESA" coating.

So now the service lifetime limitation became the Al-coated films.
They can be damaged by the incoming ionizing radiation and also by
mechanical forces associated with vacuum cycling, especially if any
air gets behind the film.


The point of this whole rant is that, if the film over the phosphor
is "cracked" or otherwise damaged, you will lose a lot of signal. SE
may land, but if the surface is negatively charged by previous SE,
they will not land with enough energy to make much light and hence
the SE signal will seem weak. This can happen so slowly that you
don't notice it unless you occasionally calibrate your SE signal from
a known clean conducting specimen (Si wafer?), with fixed kV, working
distance, etc.

A drop of silver may help but if the paint covers the center of the
scintillator where most of the signal arrives, it will prevent the SE
from reaching the phosphor and making light. i.e., use the paint
sparingly and only around the edge.

Cheers,

Jim P.

**********************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building,
FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706
JBPAWLEY-at-WISC.EDU
3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver Canada
Info: http://www.3dcourse.ubc.ca/ Applications still
being accepted
"If it ain't diffraction, it must be statistics." Anon.

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From: ck-at-ifam.fraunhofer.de
Date: Sat, 24 Mar 2007 08:43:13 -0500
Subject: [Microscopy] viaWWW: From PEELS in Tia to EELS in Digital Micrograph

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Email: ck-at-ifam.fraunhofer.de
Name: Christian Kbel

Organization: Fraunhofer IFAM

Title-Subject: [Filtered] Re: From PEELS in Tia to EELS in Digital Micrograph

Question: Hi,

It is very simply to transfer EELS data from TIA to DM. Click on the spectrum with the right mouse button, select 'export data' and select the MSA/MAS format. Just open this file in DM and all data including the calibration will be loaded.

Best regards,

Christian Kbel


-----------------------------------------
Dr. Christian Kuebel
Head Electron Microscopy

Fraunhofer IFAM
Wiener Strasse 12
28359 Bremen
Germany

mail: ckuebel-at-ifam.fraunhofer.de
web: http://www.ifam.fraunhofer.de/index.php?seite=/2804/analytik/tem/


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From: RossLM-at-missouri.edu
Date: Sun, 25 Mar 2007 17:24:22 -0500
Subject: [Microscopy] viaWWW: 2007 MAS Topical Workshop Postponed

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The 2007 MAS Topical Workshop on Hyperspectral Imaging (HI-II) has been postponed until October. You can find the new dates on the workshop home page:

http://www.microprobe.org/workshops/HI-II/ {http://www.microprobe.org/workshops/HI-II/} . Pre-registrants were contacted last week informing them of the change, but we realize that there may be individuals who had not pre-registered by might have been planning to attend. Please forward this e-mail to anyone who is not a current member of the society - along with an encouragement to join! - who might have been interested in attending.

As you may be aware, HI-II is to be the first topical workshop to be held at NIST for which MAS has taken the lead. NIST has had a long string of successful workshops, which MAS has been pleased to co-sponsor, and we certainly hope to build on that success while using the venue to promote the values and objectives of MAS. One key strategic goal for the HI-II workshop was that 25% of registrants would be students and postdocs, and a key reason for postponing the workshop was disappointing pre-registration numbers from these early career scientists. The organizers expect that the M&M 2007 meeting will provide a venue to make direct contact with students and their advisors, in order to improve student representation at the October workshop. However, we challenge all MAS members to do their part in promoting MAS and its functions among early career scientists.

Sincerely yours,

Paul Kotula
HI-II co-organizer and MAS President
--
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=2227
email: rosslm-at-missouri.edu
http://www.emc.missouri.edu

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From: ramadanhany-at-gmail.com
Date: Mon, 26 Mar 2007 16:41:31 -0500
Subject: [Microscopy] Annealing tantalum.........

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Hi all,
A very quick question, I would like to anneal tantalum and I wonder if
there is a big difference between "annealing tantalum under vacuum"
and "annealing under argon atmosphere in a tube furnace". I have the
second option available and I am not sure if there is still a chance
of growing a thin oxide film, other than the native oxide thin film,
on top of tantalum surface.

Many thanks in advance

Hany

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3, 26 -- Date: Mon, 26 Mar 2007 17:41:22 -0400
3, 26 -- From: "Hany Ramadan" {ramadanhany-at-gmail.com}
3, 26 -- To: Microscopy-at-microscopy.com
3, 26 -- Subject: Annealing tantalum.........
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From: smalinskas-at-yahoo.com
Date: Mon, 26 Mar 2007 17:57:13 -0500
Subject: [Microscopy] Re: Annealing tantalum.........

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Hany,

At temperature, tantalum has a very high affinity for
oxygen. The accepted practise is to anneal tantalum
under vacuum.

First, the tantalum must be clean. It should be
degreased, then can be chemically etched with a
solution of 60 HNO3, 20 HF and 20 H2SO4.

After the tantalum is vacuum annealed, and the
temperature has dropped below 1000C, the chamber can
be backfilled with 15 mm Hg high-purity (99.995%)
argon.

It must cool to below 200C before removing from
furnace.

Stu Smalinskas, P.E.
Metallurgist
SKF USA
Plymouth, Michigan

--- ramadanhany-at-gmail.com wrote:

}
} Hi all,
} A very quick question, I would like to anneal
} tantalum and I wonder if
} there is a big difference between "annealing
} tantalum under vacuum"
} and "annealing under argon atmosphere in a tube
} furnace". I have the
} second option available and I am not sure if there
} is still a chance
} of growing a thin oxide film, other than the native
} oxide thin film,
} on top of tantalum surface.
}
} Many thanks in advance
}
} Hany
}




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From: bressan-at-smt.zeiss.com
Date: Mon, 26 Mar 2007 19:06:13 -0500
Subject: [Microscopy] viaWWW:Job Opening TEM FIELD SERVICE ENGINEERS

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Email: bressan-at-smt.zeiss.com
Name: Beth Bressan

Organization: Carl Zeiss SMT Inc.

Title-Subject: [Filtered] Job Opening

Question: TEM FIELD SERVICE ENGINEERS

Due to expansion of our product portfolio, Carl Zeiss SMT Inc. seeks experienced TEM field service engineers w/apps experience in NY, CA & OH Engineers are responsible for maintenance, defect finding, repair & install of TEMs. A technical degree, Windows & knowledge of physics & vacuum technology is needed. We offer a competitive salary & comprehensive benefits package as well as a promising career opportunity. Send your resume in confidence to: bressan-at-smt.zeiss.com. EOE M/F




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From: bressan-at-smt.zeiss.com
Date: Mon, 26 Mar 2007 19:06:41 -0500
Subject: [Microscopy] viaWWW: Job Opening Applications Engineer

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Email: bressan-at-smt.zeiss.com
Name: Beth Bressan

Organization: Carl Zeiss SMT Inc.

Title-Subject: [Filtered] Applications Engineer Position

Question: Carl Zeiss SMT, the industry leader in e-beam repair products for the mask industry, has an immediate opening for a application specialist in Boise, ID.The sucessful candidate needs an understanding of ebeam technology & a technical bachelors degree. Responsibilities include obtaining market knowledge for all Zeiss SMS ebeam products, supporting customers in their application knowledge, ensuring source & final acceptance of ebeam products,assisting service, managing the escalation process to ensure customer problems are resolved efficiently & assisting in the selling process. Salary is commensurate with experience. Send resume in confidence to: bressan-at-smt.zeiss.com EEO M/F

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From: Aleksandr.Mironov-at-manchester.ac.uk
Date: Tue, 27 Mar 2007 08:24:42 -0500
Subject: [Microscopy] viaWWW: TEM: immunoTEM and BSA

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Email: Aleksandr.Mironov-at-manchester.ac.uk
Name: Aleksandr Mironov

Organization: University of Manchester

Title-Subject: [Filtered] TEM: immunoTEM and BSA

Question: Dear listers,

BSA is often used as a blocking agent in immunoEM protocols (fraction V as I remember). However, there are many kinds of BSA products like: cold ethanol precipitated, heat-shocked processed, IgG free, globulin free, fatty acid free. Are there any differences between them that matter in immunolabelling? Or just buy the cheapest one and it will be OK?

Sincerely,

Aleksandr Mironov
Experimental Officer
EM Unit, Faculty of Life Sciences
University of Manchester
M13 9PT
UK

E-mail: Aleksandr.Mironov-at-manchester.ac.uk

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From: mboucher-at-umn.edu
Date: Tue, 27 Mar 2007 08:32:07 -0500
Subject: [Microscopy] Position open: Electron Microscopy Specialist

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We are advertising for a Electron Microscopy Specialist.
Please see our ad on the MSA web site at:
http://www.microscopy.org/MSAUnits/PlacementOffice/JobListings.html

or go to the University of MN job site link for more details:
http://employment.umn.edu/applicants/Central?quickFind=60679

or you can contact me directly for more information.

Mike
******************************************************************
Michael L. Boucher Sr. mboucher-at-tc.umn.edu
Lab Manager Rm 18 Office Ph 612-624-6590
I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
MiNTeC node of the National Nanotechnology Infrastructure Network

University of MN Fax 612-625-5368
12 Shepherd Labs
100 Union Street S.E.
Minneapolis, MN 55455 http://www.charfac.umn.edu
********************************************************************



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From: phillipst-at-missouri.edu
Date: Tue, 27 Mar 2007 10:38:21 -0500
Subject: [Microscopy] Re: viaWWW: TEM: immunoTEM and BSA

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Let me start by admitting I have not actually done a rigorous test of this,
but I generally buy the IgG free version for all my immunocytochemistry
work. I do a lot of immuno-staining of 0.5 um semi-thick sections on glass
slides and a fair amount of EM grids and a bottle lasts a long time. If I
was doing several Western blots a day, I would be more concerned with
cost. If you look at the specifications of many "high quality" BSA's, they
are 97-99% pure. That is a great level of purity for many applications but
the 1-3% impurities are generally immunoglobulin. IgG is 15% of the
protein in human serum. If you are using secondary antibodies that don't
cross react with bovine IgG, it might be unimportant. If you are using
protein A or protein G, it could start to be significant. Good luck, Tom


At 08:27 AM 03/27/07, you wrote:



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From: tbargar-at-unmc.edu
Date: Tue, 27 Mar 2007 14:54:47 -0500
Subject: [Microscopy] vibratome use

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I would like to get in contact with someone who has experience using a
vibratome.


Tom Bargar
University of Nebraska Medical Center
Core Electron Microscopy Research Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


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From: mdufraine-at-ebsciences.com
Date: Tue, 27 Mar 2007 15:10:35 -0500
Subject: [Microscopy] Re: vibratome use

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Tom-

Energy Beam Sciences is a distributor of the Vibratome line of
equipment, if you contact me
I'm sure we can help,and/or put you in contact with a user.

Mike Dufraine

tbargar-at-unmc.edu wrote:

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TEL 800-992-9037 X340
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From: rcmoretz-at-gmail.com
Date: Tue, 27 Mar 2007 15:13:10 -0500
Subject: [Microscopy] Re: vibratome use

Contents Retrieved from Microscopy Listserver Archives
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What do you need, Tom? I have used the vibratome quite a bit and can
offer some general help but it would help if you can be more specific.

Roger Moretz, Ph.D.
Dept. of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
Ridgefield, CT

On 3/27/07, tbargar-at-unmc.edu {tbargar-at-unmc.edu} wrote:
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} I would like to get in contact with someone who has experience using a
} vibratome.
}
}
} Tom Bargar
} University of Nebraska Medical Center
} Core Electron Microscopy Research Facility
} 986395 Nebraska Medical Center
} Omaha, NE 68198-6395
} 402-559-7347
} tbargar-at-unmc.edu
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From: jmkrupp-at-ucsc.edu
Date: Tue, 27 Mar 2007 17:46:46 -0500
Subject: [Microscopy] Digital camera to stereoscope

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Hi:

OK, so I am lazy and want to mine the group before doing it the hard way.

I have an old Wild M5A that had a Wild film camera on it. Now
everyone wants to use their digital camera in place of the old film
camera.

I have resisted getting a digital set up because the market was so
fluid. I tried once, but by the time I figured everything out, the
camera was obsolete and I needed a new computer to run it anyway.

So now I have a user who has a nice Nikon digital SLR who wants to
hook it up to the Wild.

I need some guidance helping her find the right adapters. She got a
T-mount, but that only goes halfway to the solution. The camera tube
on the Wild is something like 35 mm diameter. I have a C-mount
adapter for a video camera that fits this scope. What do I need to
get her SLR to work?

Thanks

Jon
--

Jonathan Krupp
Microscopy & Imaging Lab
C230 Earth & Marine Science
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-ucsc.edu

I am riding in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise
money for the San Francisco AIDS Foundation. Visit
http://www.aidslifecycle.org for more information about the ride or
http://www.aidslifecycle.org/5482 to make a donation.

==============================Original Headers==============================
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From: marty.schreck-at-thermofisher.com
Date: Tue, 27 Mar 2007 18:31:52 -0500
Subject: [Microscopy] viaWWW: Job Opening Application Scientist (East Coast)

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Email: marty.schreck-at-thermofisher.com
Name: Marty Schreck

Organization: Thermo Fisher Scientific

Title-Subject: [Filtered] Application Scientist (East Coast)

Question: This is a key position within our organization. The individual will be responsible primarily for providing application support to showcase our complete line of EDS (Microanalysis) and Energy Dispersive X-ray Fluorescence (EDXRF) products. Prior experience using EDS, SEM and EDXRF techniques as well as other metrology techniques will be important. The prime roles will include generating application data and reports to be used in promoting our EDS and X-ray systems, working with customers and the local sales engineer to provide proof data to enable funding justification and generating novel application ideas for utilizing our EDS and X-ray technology. It is also expected that the individual will aide in visiting potential customers with the sales engineer to review technical requirements related to the purchasing of our product, along with performing demos at the customer site or demo facility. Contribute to providing scientific presentations in collaboration with our customers as well as on their own at seminars and conferences. A minimum of a BS in Scientific Field, 2 or more years experience in customer support is required although a applicant with an Associates Degree and 5 years experience in the SEM and XRF field will be considered. This position requires outstanding written and oral communication skills. Excellent software skills and knowledge of Microsoft Office as well as Statistical Control packages is expected.

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From: stmccart-at-vt.edu
Date: Wed, 28 Mar 2007 08:12:04 -0500
Subject: [Microscopy] Job Opening, Research Associate at VA Tech

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Virginia Tech is seeking an Instrument specialist for its new Nanoscale
Characterization and Fabrication Laboratory. The selected candidate will:
Maintain and operate the X-ray Photoelectron Spectrometer (XPS) and
Secondary Ion Mass Spectrometer (SIMS) in the NCFL. Keep the laboratory up
to date in the methods and techniques used with these instruments. Perform
TEM and FIB analyses and provide assessments of the results, support
individual research with training on the instruments and guidance on the
techniques. Provide analytical service to industrial clients.

For more information or to apply, go to www.jobs.vt.edu, posting number
070280. Complete the faculty application and attach a cover letter, resume
or CV, and a list of professional references. Individuals desiring
accommodation in the application process should notify Christie Thompson,
cthomp-at-vt.edu or 540-231-5495. Review of applications will begin on April
5, 2007.


Stephen McCartney
Senior Research Associate
Macromolecules and Interfaces Institute
2108 Hahn Hall
Va Tech
Blacksburg, VA 24061
540-231-9765 - phone
540-231-8517 - FAX


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From: edelmare-at-muohio.edu
Date: Wed, 28 Mar 2007 15:14:55 -0500
Subject: [Microscopy] Re: Digital camera to stereoscope

Contents Retrieved from Microscopy Listserver Archives
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Jon:

We have a poster coming up at M&M 2007 for this very thing . . .

But you want an answer now. The Nikon: I assume this is one of the
SLR's (i.e. D50, D80, D100, D200?). Note the D50 shakes due to
shutter/mirror slap and will be a problem at higher mags. Get the
Nikon software and USB cable, run directly through the computer
(rather than collecting on flashcard) - well worth the $100.

You are right you need to use the 35mm mount actually an F-mount
(not T-mount) You can get a T- to Nikon (F-) adapter at a Photo shop
BHPhoto.com has them ~ $15-20 get a better one.

OR you need the mount from the photo port on the Wild to F-mount?
Do you have any Photoeyepieces?

Unless you can find the 35-mm camera parts in a drawer somewhere or
someone else on the list getting Wild parts will be hard. However,
Diagnostics seems to make pretty good couplers.

See: http://www.diaginc.com/accessories/coupler/CouplerPA.shtml




On 27 Mar 2007 at 17:47, jmkrupp-at-ucsc.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
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} ----------------------------------------------------------------------------
}
} Hi:
}
} OK, so I am lazy and want to mine the group before doing it the hard way.
}
} I have an old Wild M5A that had a Wild film camera on it. Now
} everyone wants to use their digital camera in place of the old film
} camera.
}
} I have resisted getting a digital set up because the market was so
} fluid. I tried once, but by the time I figured everything out, the
} camera was obsolete and I needed a new computer to run it anyway.
}
} So now I have a user who has a nice Nikon digital SLR who wants to
} hook it up to the Wild.
}
} I need some guidance helping her find the right adapters. She got a
} T-mount, but that only goes halfway to the solution. The camera tube
} on the Wild is something like 35 mm diameter. I have a C-mount
} adapter for a video camera that fits this scope. What do I need to
} get her SLR to work?
}
} Thanks
}
} Jon
} --
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} C230 Earth & Marine Science
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-ucsc.edu
}
} I am riding in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise
} money for the San Francisco AIDS Foundation. Visit
} http://www.aidslifecycle.org for more information about the ride or
} http://www.aidslifecycle.org/5482 to make a donation.
}
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Richard E. Edelmann, Ph.D.
EXPO Editor, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu


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From: bfoster-at-mme1.com
Date: Wed, 28 Mar 2007 15:35:06 -0500
Subject: [Microscopy] Digital camera to stereoscope

Contents Retrieved from Microscopy Listserver Archives
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Hi, Jon

Also, you want to remember that YOU see stereo because of your brain
combining two images which come from a separation of approximately
12-15 degrees. Please warn your user that the image collected
through the photographic system will only be "mono", not
stereo. This difference in viewpoint causes a lot of frustration for
folks beginning photography through a stereo microscope, whether it
be to film or digital format.

Hope this was helpful!

Barbara Foster, President

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com


MME is now scheduling customized, on-site courses through July. Call
us today for details.

P. S.
Need a good general reference or light microscopy text for next
semester? Call us today to learn more about "Optimizing LIght
Microscopy". Copies still available through MME... even for
class-room lots ... and we give quantity discounts. Call Ken Piel at
(972)954-8011.


At 02:20 PM 3/28/2007, edelmare-at-muohio.edu wrote:



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From: cammer-at-aecom.yu.edu
Date: Wed, 28 Mar 2007 16:01:48 -0500
Subject: [Microscopy] Digital camera to stereoscope

Contents Retrieved from Microscopy Listserver Archives
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The D70 moves too because of the shutter.

I don't know whether the D80 moves or not, but as a camera, it's a
lot better than the D70.

We found that people found the D70 unusable not just because the
shutter/mirror made the microscope shake, but because people just can't focus.

They much prefer the lower resolution Axiocam that they can focus on
the screen. For most users, convenience trumps quality.

-Michael

At 03:16 PM 03/28/07, you wrote:
} SLR's (i.e. D50, D80, D100, D200?). Note the D50 shakes due to
} shutter/mirror slap and will be a problem at higher mags. Get the

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
URL: http://www.aecom.yu.edu/aif/


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From: chumbley-at-iastate.edu
Date: Wed, 28 Mar 2007 18:12:07 -0500
Subject: [Microscopy] viaWWW: Looking for used TEM

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Email: chumbley-at-iastate.edu
Name: Scott Chumbley

Organization: Iowa State University

Title-Subject: [Filtered] Looking for used TEM

Question: Does anyone have a used Philips / FEI CM-type TEM they are looking to get rid of? ISU is looking to buy a CM12, CM20 or CM30 in case somebody has upgraded to a newer instrument and is looking to sell their older microscope.

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From: hfong11-at-yahoo.com
Date: Wed, 28 Mar 2007 18:12:36 -0500
Subject: [Microscopy] viaWWW: Parts for TEM and Ion Mill

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Email: hfong11-at-yahoo.com
Name: Hanson Fong

Organization: University of Washington

Title-Subject: [Filtered] Parts for TEM and Ion Mill

Question: The Department of Materials Sci & Engineering at University of Washington is looking for the following parts:
1. single tilt holder for Phillips EM420 (1 or more)
2. a diffusion pump for a Gatan Model 600 Dual Ion Mill

If someone has these parts in good working condition and is willing to donate or sell at a reasonable price, please contact Hanson Fong at:

hfong-at-u.washington.edu
or
hfong11-at-yahoo.com (preferred)

Thank you,
Hanson Fong, Ph. D.
University of Washington
Materials Sci & Eng
302 Roberts Hall
Box 352120
Seattle, WA 98195


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From: dang-at-oakland.edu
Date: Wed, 28 Mar 2007 18:15:58 -0500
Subject: [Microscopy] AskAMicroscopist: morphology under Confocal Microscope

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This Question was submitted to Ask-A-Microscopist by (dang-at-oakland.edu)
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Email: dang-at-oakland.edu
Name: LOAN DANG

Organization: Oakland University

Education: Graduate College

Location: Rochester Hills michigan 48309

Title: To obtain the morphology under Confocal Microscope

Question: I have sample (cartilage sample) I would like to see the structure under Confocal Microscope .
Do I need to stain the sample in order to see it .
I really appreciate your response,
Sincerely
LOAN DANG

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From: MCarlyle-at-veeco.com
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Subject: [Microscopy] SPM - Seeing at the Nanoscale conference

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From: Carol.Evered-at-warwick.ac.uk
Date: Thu, 29 Mar 2007 03:48:04 -0500
Subject: [Microscopy] TEM help with pre-embedding cells in agar

Contents Retrieved from Microscopy Listserver Archives
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I have been given a protocol for embedding Cyanobacterium synechocystis from
liquid culture, after secondary fixation the cells are embedded in 2% agar
prior to dehydration and resin embedding.
When I have tried this method before the agar has set so rapidly I have had
insufficient time to incorporate the cell suspension evenly within the agar.
Details of the type of agar, temperatures and method of incorporation are
absent so any help on the practicalities of achieving a fairly uniform
distribution of cells would be gratefully received. I usually work with the
cell suspensions in Eppendorf (micro-centrifuge) tubes.
Thanks in advance.

Carol Evered
Research Imaging
Warwick HRI
University of Warwick
Wellesbourne
Warwick
CV35 9EF
UK

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From: frank.karl-at-degussa.com
Date: Thu, 29 Mar 2007 07:42:34 -0500
Subject: [Microscopy] Digital cameras and the TEM

Contents Retrieved from Microscopy Listserver Archives
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Carol

I have normally just embedded a spun down pellet in agar so the problem
of dispersing is not normally an issue.

I have read however of a method that involves:
1. melt 2% agar and then store in 50 deg C water bath
2. take a fixed and washed pellet (in eppendorf) and warm in 50 deg C
water bath
3. add warm agar to pellet and re-suspend
4. then leave for 5 mins in water bath
5. spin down rapidly 30 secs to 1 min - any longer and agar may set too
soon. I am not sure if you want to keep yours dispersed though.
6. cool in refrigerator or ice bath
7. chop up agar as required

The original method is:
Hirsch JG & Fedorko ME (1968), Journal
of Cell Biology 38:615.

But is cited in a large double
volume:
Procedures in Electron Microscopy
A.W. Robards & A.J. Wilson
(editors)
Pub 1993 John Wiley
ISBN 0 471 92853 4
pages 5:9.3-4

There are
different melting point agars so it might be possible to experiment with
temperatures or you could even try acrylamide gels which are cold setting.

Hope this helps.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk

----- Original Message -----
X-from: Carol.Evered-at-warwick.ac.uk

Let me float an question to the list. What is the effect of changing a one
meg camera to a three meg camera on a TEM?

I'm asked some fundamental questions, and I can't seem to answer them to
this person's satisfaction, which implies I don't have a good grasp of the
question or answers.

My immediate response is you would increase the resolution. I can
envision the image size on the monitor changing, but if the resolution of
the screen is lower than the captured image and if the computer/imaging
software wants to display all the image captured, will not any feature at a
specific magnification have the resolution of the monitor? It seems the
same is true for the printer. I can't simply expand the size of the paper
at will, so the software will either reduce the printed image magnification
or print just a smaller section of the total image. Again, since the
printer has a fixed resolution will not the printed image resolution will
be limited by the printer's (This sounds like a Hi-Fi discussion from the
early 60's... just change the words...) upper limit?

So why capture high resolution images? My response is it allows you to
post process and expand the image to examine one feature and have
sufficient "stored" resolution to display the image without empty
magnification. This also implies (to me at least) if I want to measure
from point A to point B, the more camera pixels I have the better I can
resolve where point A starts and point B ends which should allow me to have
better confidence in my measurements. I believe that imaging software
works on the image in memory and not the image displayed on the screen so
size of the print or screen has little to do with data obtained. It's more
a function of the size of the captured image?

Lastly.....

If I feel the need to have at least 1000 pixels in an image feature, and
due to my camera I can only capture 500 at a magnification X, is there any
reason not to simply increase the magnification so I have a larger feature
which now occupies more pixels. I realize I haven't increased the
resolution, but if my software need 999 pixels to "recognize" a feature
haven't I met this requirement?

Thank in advance!
Frank (I miss film) Karl




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From: dsherman-at-purdue.edu
Date: Thu, 29 Mar 2007 08:55:34 -0500
Subject: [Microscopy] Re: TEM help with pre-embedding cells in agar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Carol,

You should try a low temperature gelling agarose. We use Sigma Type VII
regularly. Keep the agarose at about 40oC until needed. Fix the cells as
desired while in suspension. Use the agarose as the last step prior to
dehydration so you are sure all cells are fully exposed to fix and washing.
Spin the cells down in the Eppendorf tube and remove the supernatant. Then
add ~0.5 ml agarose. Gently stir the cells up a little to get the agarose
to enrobe them while keeping the tubes in warm water so the agarose remains
liquid. Spin and the cells should have plenty of time to pellet again
before the agarose sets.

Trick is to not try to resuspend the cells completely in the agarose or the
ones at the top will not have time to get to the bottom before the agarose
sets up.

We then cool the tubes in ice (or very cool water) for a few minutes. Inject
water or low percentage ETOH toward the bottom of the tube by slipping the
pipette down the side of the agarose plug. If you do this gently you can get
the plug to release and float up so that it can be dumped out and then the
pellet sliced into appropriate sized pieces for dehydration and
infiltration.

Debby

Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy


} From: {Carol.Evered-at-warwick.ac.uk}
} Reply-To: {Carol.Evered-at-warwick.ac.uk}
} Date: Thu, 29 Mar 2007 03:50:39 -0500
} To: {dsherman-at-purdue.edu}
} Subject: [Microscopy] TEM help with pre-embedding cells in agar
}
}
}
}
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} I have been given a protocol for embedding Cyanobacterium synechocystis from
} liquid culture, after secondary fixation the cells are embedded in 2% agar
} prior to dehydration and resin embedding.
} When I have tried this method before the agar has set so rapidly I have had
} insufficient time to incorporate the cell suspension evenly within the agar.
} Details of the type of agar, temperatures and method of incorporation are
} absent so any help on the practicalities of achieving a fairly uniform
} distribution of cells would be gratefully received. I usually work with the
} cell suspensions in Eppendorf (micro-centrifuge) tubes.
} Thanks in advance.
}
} Carol Evered
} Research Imaging
} Warwick HRI
} University of Warwick
} Wellesbourne
} Warwick
} CV35 9EF
} UK
}
} ==============================Original Headers==============================
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From: Elliott-at-arizona.edu
Date: Thu, 29 Mar 2007 10:36:58 -0500
Subject: [Microscopy] Re: TEM help with pre-embedding cells in agar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Carol

I have done this many times. I use Low Melting point Agarose. Heat
the Eppendorph tube (with the bugs in it) up to 37-40C. Mix your
bugs and agarose in the warm tube. Once the bugs are mixed (pipet up
and down a few times) then put the sample on ice to cool the bugs and
harden the agarose.
If you need to, you can heat the tube more, which will give you more
time to work until the agarose hardens. If you work fast and ice the
sample immediately after working then the increased temp has not
caused me problems.

FYI I have used the same technique for many kinds of suspended cells.

I have found this to work well.

David



_____________________

David Elliott Ph.D.
Assistant Professor - Department of Cell Biology and Anatomy
Director, Research Microscopy Core Service
University of Arizona College of Medicine
PO Box 245004
Tucson, AZ 85724

Voice: 520-626-7870
Fax: 520-626-2097


On Mar 29, 2007, at 1:51 AM, Carol.Evered-at-warwick.ac.uk wrote:

}
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} ----------------------------------------------------------------------
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} I have been given a protocol for embedding Cyanobacterium
} synechocystis from
} liquid culture, after secondary fixation the cells are embedded in
} 2% agar
} prior to dehydration and resin embedding.
} When I have tried this method before the agar has set so rapidly I
} have had
} insufficient time to incorporate the cell suspension evenly within
} the agar.
} Details of the type of agar, temperatures and method of
} incorporation are
} absent so any help on the practicalities of achieving a fairly uniform
} distribution of cells would be gratefully received. I usually work
} with the
} cell suspensions in Eppendorf (micro-centrifuge) tubes.
} Thanks in advance.
}
} Carol Evered
} Research Imaging
} Warwick HRI
} University of Warwick
} Wellesbourne
} Warwick
} CV35 9EF
} UK
}
} ==============================Original
} Headers==============================
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From: cervantes-at-bendres.com
Date: Thu, 29 Mar 2007 10:49:49 -0500
Subject: [Microscopy] TEM help with pre-embedding cells in agar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Carol -
Yet another protocol for agarose embedding is this one, that I got from the University of Bristol Veterinary Pathology website (http://www.bristol.ac.uk/vetpath/cpl/emtechs.htm):

Prepare a 1.5% Solution of Agarose (Sigma Type VII is what I use) in distilled water by bringing to the boil while stirring.
Spin samples at 5,000 rpm for 5 minutes. Decant supernatant from sample tubes and take them and the agar solution to the centrifuge.
When the agar has cooled to ~60C, quickly fill each tube with it, resuspend the samples (vortex briefly) and spin them at full speed for 30 seconds to 1 minute (I do a minute at 13,000 rpm).
Do a max of 4 at a time or the agar will set before the sample can be spun down to the bottom of the tube.
Cool the tubes by putting in a fridge (or you can use a beaker of ice water).

I remove the agar plug by cutting the Eppendorf tube side with a razor blade and pulling out the agar. However, this is not the safest procedure, and I like Debbie Sherman's suggestion about using water or EtOH to remove the plug. I then cut off the end containing the sample, and cut up the sample end into cubes.

Jessica Cervantes
Bend Research Inc
Bend, Oregon 97701

-----Original Message-----
X-from: Carol.Evered-at-warwick.ac.uk [mailto:Carol.Evered-at-warwick.ac.uk]
Sent: Thursday, March 29, 2007 1:55 AM
To: Cervantes, Jessica

I have been given a protocol for embedding Cyanobacterium synechocystis from
liquid culture, after secondary fixation the cells are embedded in 2% agar
prior to dehydration and resin embedding.
When I have tried this method before the agar has set so rapidly I have had
insufficient time to incorporate the cell suspension evenly within the agar.
Details of the type of agar, temperatures and method of incorporation are
absent so any help on the practicalities of achieving a fairly uniform
distribution of cells would be gratefully received. I usually work with the
cell suspensions in Eppendorf (micro-centrifuge) tubes.
Thanks in advance.

Carol Evered
Research Imaging
Warwick HRI
University of Warwick
Wellesbourne
Warwick
CV35 9EF
UK

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From: gmartens-at-interchange.ubc.ca
Date: Thu, 29 Mar 2007 10:57:55 -0500
Subject: [Microscopy] Re: TEM help with pre-embedding cells in agar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Carol,

We have also done this technique several times with great success
using both low melting point agarose and sometimes using 12% gelatin.
The concern with using agarose is the potential to introduce bubbles
which sometimes don't move during centrifugation. Using gelatin is
sometimes easier because of the lower viscosity, even at 12% and as
long as you keep the gelatin below 30 C it will stay solid. We buy
our gelatin from the supermarket, yes, the same stuff used for
cooking and making jellies (we use Knox gelatin). This evolved from
using gelatin for the Tokuyasu technique.

Good luck,

Garnet

--
Garnet Martens

Research Manager
BioImaging Facility
University of British Columbia
6270 University Blvd.
Vancouver, B.C.
Canada
V6T 1Z4

phone 604-822-3354

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From: Mike.Bode-at-olympus-sis.com
Date: Thu, 29 Mar 2007 11:03:02 -0500
Subject: [Microscopy] Digital cameras and the TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Frank,

Perhaps this can help:

A digital TEM camera essentially consists of a screen that converts
electrons to photons, and a camera system that takes images of that
screen. There are several resolution limiting elements in this chain.
The fist is the TEM itself. Let's say it has a resolution of 0.2 nm.
Then there is the phosophor screen (in most cases) that converts the
electrons to photons. Each electron scatters in the screen and creates
multiple photons. The efect is that a single electron creates a light
spot of a size that depends on the thickness and composition of the
film, the accelerating voltage, etc. For simplicity, let's say that this
light spot is 10 microns. Finally, you have the camera system that
records these light spots. It has a resolution itself, which depends on
pixel size and optics. All of this is governed by the Nyquist or Shannon
theorem which set theoretical limits to the resolution.

The critical part in this chain is the phosphor. If you have a 10 micron
resolution, and you are working at, let's say, 1000x, a 10 micron spot
on the phosphor will show a roughly 10 nm spot of the sample. In other
words, in this situation your resolution will be limited to 10 nm, no
matter how good the resolution of your TEM is. Only if you work above
50,000X does the resolution of the microscope start playing a role.

So, if you have a camera that is designed for highest resolution
(meaning that it can record all the information that comes from the
phosphor at the Nyqust limit), and you increase the number of pixels,
you will not gain an increase in resolution, but in field of view. If,
on the other hand, the camera was designed for maximum field of view,
and you keep the field of view, an increase in the number of pixels
might result in an increase in resolution, if the smaller camera did not
meet the Nyquist limit. This is the case as long as the resolution
limiting factor is the phosphor. If you go way up in magnification, the
resolution limiting factor might be the TEM, and in those cases it is
possible that adding pixels only adds empty resolution.

As far as your "1000" pixel question is concerned, the answer is again
2-fold. If you are in a magnification range where the phosphor is the
limiting factor, an increase in magnification will also increase the
resolution. If you are in a range where the TEM limits the resolution,
you will only get a larger image of the same blurry blob, and sacrifice
field of view. In that case it would be better to take the "500 pixel"
image, and simply have a computer interpolate the data to increase the
number of pixels and then use that as a source of the analysis. (you
would have to document that, though, to avoid charges of "unethical
image processing")




Michael Bode, Ph.D.

General Manager

OLYMPUS SOFT IMAGING SOLUTIONS
12596 West Bayaud Ave #300
Lakewood, CO 80228
USA
Tel.: +1 (303) 234-9270
Fax.: +1 (303) 234-9271
E-mail: Mike.Bode-at-olympus-sis.com
www.olympus-sis.com

-----Original Message-----
X-from: frank.karl-at-degussa.com [mailto:frank.karl-at-degussa.com]
Sent: Thursday, March 29, 2007 06:49
To: Mike Bode

Let me float an question to the list. What is the effect of changing a
one meg camera to a three meg camera on a TEM?

I'm asked some fundamental questions, and I can't seem to answer them
to this person's satisfaction, which implies I don't have a good grasp
of the question or answers.

My immediate response is you would increase the resolution. I can
envision the image size on the monitor changing, but if the resolution
of the screen is lower than the captured image and if the
computer/imaging software wants to display all the image captured, will
not any feature at a
specific magnification have the resolution of the monitor? It seems
the
same is true for the printer. I can't simply expand the size of the
paper at will, so the software will either reduce the printed image
magnification or print just a smaller section of the total image.
Again, since the printer has a fixed resolution will not the printed
image resolution will be limited by the printer's (This sounds like a
Hi-Fi discussion from the early 60's... just change the words...) upper
limit?

So why capture high resolution images? My response is it allows you to
post process and expand the image to examine one feature and have
sufficient "stored" resolution to display the image without empty
magnification. This also implies (to me at least) if I want to measure
from point A to point B, the more camera pixels I have the better I can
resolve where point A starts and point B ends which should allow me to
have better confidence in my measurements. I believe that imaging
software works on the image in memory and not the image displayed on the
screen so size of the print or screen has little to do with data
obtained. It's more a function of the size of the captured image?

Lastly.....

If I feel the need to have at least 1000 pixels in an image feature, and
due to my camera I can only capture 500 at a magnification X, is there
any reason not to simply increase the magnification so I have a larger
feature which now occupies more pixels. I realize I haven't increased
the resolution, but if my software need 999 pixels to "recognize" a
feature haven't I met this requirement?

Thank in advance!
Frank (I miss film) Karl




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From: PWebster-at-hei.org
Date: Thu, 29 Mar 2007 11:50:58 -0500
Subject: [Microscopy] TEM help with pre-embedding cells in agar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Gelatin or agarose can be used to support cell suspensions for subsequent
sectioning. One advantage of gelatin over agarose is that if the gel sets
before the cells have been pelleted down, the gelatin can be easily
liquefied by warming to 37 degrees. Agarose needs a little more heating to
liquefy.

One important point to remember is that if the cells have been fixed in
aldehyde, residual aldehyde has to be either removed or quenched or it will
cross-link gelatin or agarose before you are ready to let it gel. Wash the
cells in a low concentration of ammonium chloride, lycine or glycine before
embedding in the gel.

Regards,

Paul Webster.


Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
(213) 273 8026
pwebster-at-hei.org


________________________________________________________________________

Hi Carol,

We have also done this technique several times with great success
using both low melting point agarose and sometimes using 12% gelatin.
The concern with using agarose is the potential to introduce bubbles
which sometimes don't move during centrifugation. Using gelatin is
sometimes easier because of the lower viscosity, even at 12% and as
long as you keep the gelatin below 30 C it will stay solid. We buy
our gelatin from the supermarket, yes, the same stuff used for
cooking and making jellies (we use Knox gelatin). This evolved from
using gelatin for the Tokuyasu technique.

Good luck,

Garnet


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From: ahlst007-at-umn.edu
Date: Thu, 29 Mar 2007 11:59:29 -0500
Subject: [Microscopy] Re: TEM help with pre-embedding cells in agar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Carol,

I have read with interest this thread on
embedding cells in agar. The procedure I have
used successfully for years is quite close to
that described by Debby Sherman. As she and
others have pointed out, its necessary to keep
the melted agar, fixed and washed cell pellets,
pipets, tubes, etc warm to prevent premature
freezing of the agar, so it goes into the
microfuge at about 40 C. I then spin down for 10
minutes at 14,000 rpm, a bit more than others
have recommended, but I want to be sure that I
get those puppies down!

I'm not sure why you want to evenly disperse the
cells into the agar. Usually a tight, enrobed
pellet is desired so that when you view sections
you will see lots of cells fairly close
together. But it is necessary to gently mix the
cells into the agar just a little to effectively
enrobe them but without diluting the pellet too
much for the above reason.

I mix my low melting point agarose (Sigma, #
A9414) to 2% w/v, and keep my water bath for
keeping the melted agar and cell pellets warm
in 1.5 ml Eppendorf tubes at about 42C. Between
use, I store the dissolved agarose stock in the
freezer.

The only other thing I would add to this
discussion is to point out a paper by Jaqueline
Wood and Karen Klomparens in which agarose, agar
and gelatin were compared as encapsulating media
for bacteria, yeast and mitochondria. They
conclude that agarose has advantages over the
other two, mainly that it contributes the least
background density in the TEM image. As a result
of reading this paper, I switched from agar to
agarose. The reference is:

Wood, Jacqueline I. and Klomparens, Karen L.
1993. Characterization of agarose as an
encapsulation medium for particulate specimens
for transmission electron microscopy. Microscopy
Research and Technique 25:267-275.

Good luck!

Gib
---------
Gilbert (Gib) Ahlstrand, Electron Microscopist,
Imaging Center, University of Minnesota
123 Snyder Hall
St. Paul, MN 55108
http://www.cbs.umn.edu/ic
----------

Carol.Evered-at-warwick.ac.uk wrote:
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} I have been given a protocol for embedding Cyanobacterium synechocystis from
} liquid culture, after secondary fixation the cells are embedded in 2% agar
} prior to dehydration and resin embedding.
} When I have tried this method before the agar has set so rapidly I have had
} insufficient time to incorporate the cell suspension evenly within the agar.
} Details of the type of agar, temperatures and method of incorporation are
} absent so any help on the practicalities of achieving a fairly uniform
} distribution of cells would be gratefully received. I usually work with the
} cell suspensions in Eppendorf (micro-centrifuge) tubes.
} Thanks in advance.
}
} Carol Evered
} Research Imaging
} Warwick HRI
} University of Warwick
} Wellesbourne
} Warwick
} CV35 9EF
} UK
}

==============================Original Headers==============================
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From: dac-at-research.umass.edu
Date: Thu, 29 Mar 2007 13:08:34 -0500
Subject: [Microscopy] Re: TEM help with pre-embedding cells in agar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Carol,

I would just like to add that we use 2% "ultra-low gelling agarose"
Sigma Type IX. Mix in 2x strength buffer and use vol equal to cell
suspension. This agarose melts at ~50C but only gels at 8C-17C, allowing
you to work with tissues at room temp and then gel when you are ready by
placing on ice or in refrigerator a few minutes. For very sensitive
materials, working at ~25C may be an advantage. In other respects it is
handled just as detailed in all the other replies. I use a piece of
sheet Teflon from Small Parts Inc. to work on and the agarose drops
easily float off when set.

Dale

Carol.Evered-at-warwick.ac.uk wrote:
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} ----------------------------------------------------------------------------
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} I have been given a protocol for embedding Cyanobacterium synechocystis from
} liquid culture, after secondary fixation the cells are embedded in 2% agar
} prior to dehydration and resin embedding.
} When I have tried this method before the agar has set so rapidly I have had
} insufficient time to incorporate the cell suspension evenly within the agar.
} Details of the type of agar, temperatures and method of incorporation are
} absent so any help on the practicalities of achieving a fairly uniform
} distribution of cells would be gratefully received. I usually work with the
} cell suspensions in Eppendorf (micro-centrifuge) tubes.
} Thanks in advance.
}
} Carol Evered
} Research Imaging
} Warwick HRI
} University of Warwick
} Wellesbourne
} Warwick
} CV35 9EF
} UK
}
} ==============================Original Headers==============================
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From: sergei2-at-ornl.gov
Date: Thu, 29 Mar 2007 14:14:03 -0500
Subject: [Microscopy] MRS Fall 2007 - Symposium on Scanning Probe Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues
We would like to bring to your attention the Symposium on
Scanning Probe Microscopy applications for characterization of nanoscale
phenomena in functional nanomaterials, to be held at the Materials
Research Society 2007 Fall meeting in Boston, MA. The full text for call
for papers is available at
http://www.mrs.org/s_mrs/sec.asp?CID=8005&DID=192095 and is also listed
below. The deadline for abstract submission is June 20.
Looking forward to seeing you in Boston!
On behalf of the organizers
Sergei V. Kalinin

MRS 2007 Fall meeting - Symposium B

Nanoscale Phenomena in Functional Materials by Scanning Probe Microscopy

The last decade has witnessed spectacular progress in the development
and applications of scanning probe microscopy (SPM)-based nanoscale
imaging techniques. The combination of high spatial resolution and
sensitivity to local electronic, optical, and mechanical properties
places these techniques among the most versatile tools for nanoscience,
biology, physics, and materials science. Atomic and electronic structure
of surfaces, vibrational excitations, energy flow, and local materials
properties on the molecular level has become accessible with the advent
of high-resolution SPMs. Electrostatic SPMs are being established as
powerful techniques for spatially resolved studies of electronic
transport on the nanometer level at electroactive interfaces and in
molecular electronic devices such as carbon nanotubes. Dynamic SPM modes
and scanning indentation techniques allow mechanical compliance and
surface energy to be investigated at the nanoscale with applications to
the emerging fields of nanotribology, nanofluidics, nanocomposites, and
NEMS. Novel optical imaging modes, such as tip-enhanced spectroscopy,
solid immersion microscopy, and apertureless scanning optical
microscopy, have joined the now-established near-field scanning optical
microscopy (NSOM), bringing the resolution of optical spectroscopy into
the nanoscale regime and complementing local electronic measurements of
materials with the STM family of instruments. These new measurement
techniques were necessitated by the growing need for materials
characterization on the nanoscale and have in turn led to the discovery
of new nanoscale phenomena. Finally, the SPM has been used to manipulate
and fabricate materials at the nanoscale.

It is the goal of this symposium to provide a multidisciplinary forum
for scanning-probe-based materials and nanoscience in order to
demonstrate the latest achievements in technique developments and
materials applications that have led to scientific discoveries. The
symposium will include two types of sessions: One will be dedicated to
the recent advances in technique development of interest to the
materials community and will bring together specialists in practical and
theoretical aspects of SPM imaging. The second will focus on specific
materials-related phenomena, including nanotubes and nanowires, quantum
dots, surfaces, interfaces, and biological systems studied by local
probe techniques.

The topics of the symposium will include, but not be limited to:

- Imaging, manipulation, and energy transfer on the atomic and molecular
level by atomic resolution NC-AFM and STM
- Local optical and electronic properties and excitations, e.g.,
plasmons measured with SPMs
- Defects, impurities, dopants, and transport in semiconductor
nanostructures, nanotubes, and nanowires
- Mechanics and electromechanics on the nanoscale by SPM and
nanoindentation
- Mechanical and voltage nanolithography and surface modification
- Energy flows and dissipation in materials, devices, and nanostructures

- Transport in single-molecule devices and carbon nanotubes
- Imaging and characterization of ferroelectric materials
- Electronic properties of semiconductor heterostructures
- Imaging and characterization of biological systems
- Dynamics and imaging of polymers and soft materials

Invited speakers include: Robert Carpick (Univ. of Pennsylvania), Levent
Degertekin (Georgia Inst. of Technology), Dennis Discher (Univ. of
Pennsylvania), Ricardo Garcia (Univ. Madrid, Spain), Franz Giessibl
(Univ. Augsburg, Germany), Venkat Gopalan (Pennsylvania State Univ.),
Jan Hoh (Johns Hopkins Univ.), Ernesto Joselevich (Weizmann Inst. of
Science, Israel), Maki Kawai (RIKEN, Japan), L. Kuipers (FOM, The
Netherlands), Alexander Malkin (Lawrence Livermore National Lab), Lukas
Novotny (Univ. of Rochester), E. Ward Plummer (Univ. of Tennessee/Oak
Ridge National Lab), V. Sandoghdar (ETH Zurich, Switzerland), M.
Tomitori (JAIST, Japan), and S. Wilks (Swansea Univ., United Kingdom).

Symposium Organizers

Dawn Bonnell
University of Pennsylvania, Nano/Bio Interface Center, 3231 Walnut St.,
Philadelphia, PA 19104
Tel 215-898-6231, Fax 215-746-3204, bonnell-at-lrsm.upenn.edu

Sergei V. Kalinin
Oak Ridge National Laboratory, Materials Sciences and Technology
Division and Center for Nanophase Materials Sciences, 1 Bethel Valley
Rd., Oak Ridge, TN 37831
Tel 865-241-0236, Fax 865-574-4143, sergei2-at-ornl.gov

Sidney R. Cohen
Weizmann Institute of Science, Surface Analysis Laboratory, Rehovot
76100 Israel
Tel 972-8-934-2703/3422, Fax 972-8-934-4137, sidney.cohen-at-weizmann.ac.il


Richard E. Palmer
University of Birmingham, School of Physics and Astronomy, Nanoscale
Physics Research Laboratory, Birmingham B15 2TT, United Kingdom Tel
44-121-414-4653, Fax 44-121-414-7327, r.e.palmer-at-bham.ac.uk



--
Sergei V. Kalinin,
Oak Ridge National Laboratory,
1 Bethel Valley Rd,
Bldg. 3025, MS6030,
Oak Ridge, TN 37831
Phone: (865) 241-0236
FAX: (865) 574-4143
URL: sergei2.kalininweb.com
New: http://nanotransport.ornl.gov



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From: marksmsa-at-gmail.com
Date: Thu, 29 Mar 2007 18:38:10 -0500
Subject: [Microscopy] EDM 2.0.1

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Carol

As I have mentioned in an earlier post an alternative that is to
encapsulate in alginate, we have used this to encapsulate both
individual cells and tissues. The advantage is that you work at ambient
temperature (no heating), disadvantage that you introduce calcium ions
into the system which you may not want to do). One method we used was to
use a 2% solution of sodium alginate and solidify by dropping into or
flooding with 50 mM Calcium Chloride.

I have used this with plant cell suspensions in the past after the
primary fixation.

Ian

Ian Hallett
Sensory and Consumer Science - Microscopy
HortResearch, Mt Albert Research Centre
Private Bag 92 169, Auckland Mail Centre
Auckland 1142, New Zealand
+64-9-815 4200 ext 7002

-----Original Message-----
X-from: Carol.Evered-at-warwick.ac.uk [mailto:Carol.Evered-at-warwick.ac.uk]
Sent: Thursday, 29 March 2007 8:51 p.m.
To: Ian Hallett

I have been given a protocol for embedding Cyanobacterium synechocystis
from liquid culture, after secondary fixation the cells are embedded in
2% agar prior to dehydration and resin embedding.
When I have tried this method before the agar has set so rapidly I have
had insufficient time to incorporate the cell suspension evenly within
the agar.
Details of the type of agar, temperatures and method of incorporation
are absent so any help on the practicalities of achieving a fairly
uniform distribution of cells would be gratefully received. I usually
work with the cell suspensions in Eppendorf (micro-centrifuge) tubes.
Thanks in advance.

Carol Evered
Research Imaging
Warwick HRI
University of Warwick
Wellesbourne
Warwick
CV35 9EF
UK

==============================Original
Headers==============================
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"'Microscopy-at-Microscopy.Com'" {Microscopy-at-microscopy.com} 1, 26 --

Version 2.0.1 of the edm software has been officially released. The
main changes are:
1) Much better (and faster) analysis of images and diffraction patterns
2) Reasonable structure completion code
3) CBED simulation
4) Anonymous CVS, Windows versions
5) All for exactly $0.00

For details see www.numis.northwestern.edu/edm and
http://www.numis.northwestern.edu/edm/documentation/edm.htm

==============================Original Headers==============================
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From: jmastrangelo-at-ulbi.com
Date: Thu, 29 Mar 2007 20:14:23 -0500
Subject: [Microscopy] viaWWW: OM: Info needed Old Microscopes

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Email: jmastrangelo-at-ulbi.com
Name: Joe Mastrangelo

Organization: Ultralife Batteries

Title-Subject: [Filtered] Old Microscopes

Question: We have a couple of older looking microscopes gathering dust in the back of our lab that look like they may have been very nice instruments at one time. Unfortunately, I have no idea how old they are or whether they are fully operational. I have scoured the lab for the manuals, but haven't had any luck finding any useful information on them.

The microscopes are an Olympus BH-2 and a Nikon EPIPHOT.

My hope is to clean them up and get them fully operational again, but I do not have extensive experience working with these older optical microscopes. My questions to the list are as follows:

1) Does anyone have information or manuals on either of these scopes that they would be willing to share?

2) Is it possible to still obtain parts or service for these scopes, if needed?

3) The Olympus scope looks like it has a camera mounted on the top of the optical column. If I can get this scope working, will I be able to find film that still works in this camera?

Thanks so much in advance for your help. This list is a wonderful resource.

Best regards,

Joe Mastrangelo

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From: rra-at-stowers-institute.org
Date: Thu, 29 Mar 2007 20:14:47 -0500
Subject: [Microscopy] viaWWW: Leica EM-Stain

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Email: rra-at-stowers-institute.org
Name: Rhonda Allen

Organization: Stowers Institute

Title-Subject: [Filtered] Leica EM-Stain

Question: Hello, I am interested in purchasing the Leica EM-Stain. Does anyone have any comments, positive and negative, that I should take into consideration before making such an investment? We will be using it on formvar coated slot grids. Thanks in advance, Rhonda Allen BA HT(ASCP)HTL, QIHC Stowers Institute for Medical Research Kansas City, Missouri 816-926-4346


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From: bernard-at-berkeleyrc.com
Date: Thu, 29 Mar 2007 21:04:44 -0500
Subject: [Microscopy] Jeol DSG1 Plus frame grabber

Contents Retrieved from Microscopy Listserver Archives
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Anyone using this system? I'm using mine with a Jeol 6100 SEM, and
it works but there are image defects--different ones for each mode:

In raw mode, the picture is noisy.

In average mode, the noise is much better, but it has a bug in which
pure white (value 255) is instead represented as pure black (value 0).
This is an obvious bug caused by numerical "wrap around."

In integrate mode, the picture suffers from random horizontal skewing,
which both other modes have also, but it's worse in Integrate mode.

Has anyone else encounters such problems with this system and
developed a work around?

Does anyone have a software manual? Does it say more than the software's Help?

I do have the installation manual. And I know that the Olympus Adda
III would make a great replacement.

Thanks!

--
Bernard R. Cuzzillo, Ph.D., P.E.
President, Mechanical Engineer, and Fire Scientist
Berkeley Research Company (BRC)
600 Addison Street
Berkeley, CA 94710-1920
USA

bernard-at-berkeleyrc.com

Cell phone: 510.821.2499

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From: alan.wood-at-JUSTIS.COM
Date: Fri, 30 Mar 2007 02:14:18 -0500
Subject: [Microscopy] RE: viaWWW: OM: Info needed Old Microscopes

Contents Retrieved from Microscopy Listserver Archives
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Joe Mastrangelo wrote:

} The microscopes are an Olympus BH-2 and a Nikon EPIPHOT.
}
} 1) Does anyone have information or manuals on either of these
} scopes that they would be willing to share?

You can find a catalogue and a manual for the BH-2 here:

{http://www.alanwood.net/downloads/olympus-bh-2-brochure.pdf} (5.41 MB)

{http://www.alanwood.net/downloads/olympus-bh-2-bht-manual.pdf} (1.26 MB)

Alan Wood
http://www.alanwood.net (Unicode, special characters, pesticide names)

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From: Sander.Stoks-at-fei.com
Date: Fri, 30 Mar 2007 03:28:56 -0500
Subject: [Microscopy] Digital cameras and the TEM

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Hello Frank,

Most of your questions will resolve themselves once you stop using the concept of "magnification". It's a fundamental issue: When people attach "databars" to images and insist on having the "magnification" number in there, this number will be wrong for every image size but the exact size at which it was taken. If you use a bigger monitor, or display the image with a projector, the magnification is changed.

A much better measure is "pixel size in nanometer" or "horizontal field width" etc.; these numbers are properties of the _image_ and not of the _display_.

So if you use a camera with a higher "resolution" (another term which is overloaded: it can mean both "number of pixels in a sensor" and "resolving power", but I mean "more pixels" here), you are simply able to have more pixels per nanometer of image data. It may not matter much for your screen, but printers typically have a much higher resolution (in the dpi meaning), so you'll be able to print out the same image with much more detail.

Your reasoning that you can also simply increase the magnification of the microscope only works so far. In physics, you can often check the validity of an assumption by trying "edge cases" (like infinite or zero). So, take a hypothetical CCD camera with only a single pixel. You can increase the magnification of your TEM until the cows come home, but you'll never be able to take an image with sufficient data to perform your post processing with.

If your post-processing software needs "1000 pixels" (say a 33x33 square) to recognize a feature, and you have a "one kilopixel camera", then you can only take pictures of one single feature at a time, if you make sure it fills the entire sensor. If you have a 1 megapixel camera, you need not be so accurate when acquiring the images as the software can crop out the interesting areas and still have plenty of pixels to do its recognition thing.

Hope that helps,
Regards,
Sander Stoks

________________________________

X-from: frank.karl-at-degussa.com [mailto:frank.karl-at-degussa.com]
Sent: Thu 3/29/2007 2:49 PM
To: Stoks, Sander

Let me float an question to the list. What is the effect of changing a one
meg camera to a three meg camera on a TEM?

I'm asked some fundamental questions, and I can't seem to answer them to
this person's satisfaction, which implies I don't have a good grasp of the
question or answers.

My immediate response is you would increase the resolution. I can
envision the image size on the monitor changing, but if the resolution of
the screen is lower than the captured image and if the computer/imaging
software wants to display all the image captured, will not any feature at a
specific magnification have the resolution of the monitor? It seems the
same is true for the printer. I can't simply expand the size of the paper
at will, so the software will either reduce the printed image magnification
or print just a smaller section of the total image. Again, since the
printer has a fixed resolution will not the printed image resolution will
be limited by the printer's (This sounds like a Hi-Fi discussion from the
early 60's... just change the words...) upper limit?

So why capture high resolution images? My response is it allows you to
post process and expand the image to examine one feature and have
sufficient "stored" resolution to display the image without empty
magnification. This also implies (to me at least) if I want to measure
from point A to point B, the more camera pixels I have the better I can
resolve where point A starts and point B ends which should allow me to have
better confidence in my measurements. I believe that imaging software
works on the image in memory and not the image displayed on the screen so
size of the print or screen has little to do with data obtained. It's more
a function of the size of the captured image?

Lastly.....

If I feel the need to have at least 1000 pixels in an image feature, and
due to my camera I can only capture 500 at a magnification X, is there any
reason not to simply increase the magnification so I have a larger feature
which now occupies more pixels. I realize I haven't increased the
resolution, but if my software need 999 pixels to "recognize" a feature
haven't I met this requirement?

Thank in advance!
Frank (I miss film) Karl




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From: dax_uktc-at-abv.bg
Date: Fri, 30 Mar 2007 07:44:12 -0500
Subject: [Microscopy] viaWWW: Reichert Zetopan Manuals

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Email: dax_uktc-at-abv.bg
Name: Yordan Naydenov

Organization: Faculty of Biology, University of Sofia

Title-Subject: [Filtered] Reichert Zetopan Manuals

Question: Hello,

Our lab obtained a Reichert Zetopan microscope (combined with Binolux III twin lamp unit). Unfortunately, we don't have any instruction/exploitation manuals both for the microscope and the unit.
I have searched the archives of this site (http://www.microscopy.com) and found that others also had asked for such manuals. Some people had put materials in the web space but this sources are not available at present.
Could anyone provide us with such documents (in electronic format or xeroxed; preferably in English)? If so, please, contact me at dax_uktc-at-abv.bg to discuss the details of submitting.

Thanks in advance,
Yordan Naydenov

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From: rachid_sakly-at-yahoo.fr
Date: Fri, 30 Mar 2007 07:44:34 -0500
Subject: [Microscopy] viaWWW: sodium rhodizonate test

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Email: rachid_sakly-at-yahoo.fr
Name: rachid

Organization: Ecole Sup des Sci et Tech de la Sant

Title-Subject: [Filtered] sodium rhodizonate test

Question: I need to know the details of sodium rhodizonate test allowing to highlight lead crystals in testes and other organs in rats.

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From: MTLab-at-comcast.net
Date: Fri, 30 Mar 2007 08:52:51 -0500
Subject: [Microscopy] viaWWW: Manual for Robinson Detector

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Email: MTLab-at-comcast.net
Name: Roy Nelson

Organization: Material Testing Laboratory

Title-Subject: [Filtered] Manual for Robinson Detector

Question: We have started using a Robinson detector (model RDEM II) that came with a used SEM. A manual would great either in electronic or paper form. I have already searched the archives and sent an E-mail to SEMRA. Please contact me directly at MTLab-at- comcast.net or (609) 730-0575.

Thanks.

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7, 11 -- Subject: viaWWW: Manual for Robinson Detector
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From: marksmsa-at-gmail.com
Date: Fri, 30 Mar 2007 09:22:48 -0500
Subject: [Microscopy] Electron Microscopy Software Listing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The Commission on Electron Diffraction of the IUCR has setup a web
page which contains several things which may be of interest to members
of this list:

1) A listing of software for electron microscopy at
http://www.numis.northwestern.edu/IUCR_CED/software/html/ecsoftware.htm
The idea is that people can add their software to this list, which is
currently rather small.

2) Some information about different techniques at
http://www.numis.northwestern.edu/IUCR_CED/details.htm
At the moment this is very short -- contributions welcome.

3) A short list of upcoming conferences; please send information to my
real email (in signature) to add.

Other information will be added in the future, for instance how to
apply for IUCR support for workshops or schools on electron
crystallography.

--
Laurence Marks
Department of Materials Science and Engineering
MSE Rm 2036 Cook Hall
2220 N Campus Drive
Northwestern University
Evanston, IL 60208, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
email: L-marks at northwestern dot edu
Web: www.numis.northwestern.edu
EMM2007 http://ns.crys.ras.ru/EMMM07/
Commission on Electron Diffraction of IUCR
www.numis.northwestern.edu/IUCR_CED

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From: bfoster-at-mme1.com
Date: Fri, 30 Mar 2007 13:06:56 -0500
Subject: [Microscopy] Re: viaWWW: Reichert Zetopan Manuals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Yordan

I have some of these manuals (also, a Zetapan which I treasure!), but will need some time to find them. When I got my Zetapan (in the early 1980's) I had a chance to raid the Reichert office in Slough, England, for manuals, etc.

I will be out of the office until next Tuesday afternoon. If you email me next week, I'll see what I can do for you.

Best regards,

Barbara Foster, President

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com


MME is now scheduling customized, on-site courses through July. Call us today for details.

P. S.
Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.



At 12:01 PM 3/30/2007, dax_uktc-at-abv.bg wrote:



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From: gcouger-at-science-info.net
Date: Fri, 30 Mar 2007 15:35:06 -0500
Subject: [Microscopy] Re: viaWWW: Reichert Zetopan Manuals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Yordan,

I have Zetopan manuals you can download free at
www.science-info.net/docs/reichert
{http://www.science-info.net/docs/reichert/} I believe I have both
English and German copays there.

I collect manuals for old microscope and related equipment and keep for
download at:
www.science-info.net {http://www.science-info.net}

Most of the manuals come from others in a group effort to make the
available for all.


Gordon
Stillwater OK

dax_uktc-at-abv.bg wrote:
}
} Email: dax_uktc-at-abv.bg
} Name: Yordan Naydenov
}
} Organization: Faculty of Biology, University of Sofia
}
} Title-Subject: [Filtered] Reichert Zetopan Manuals
}
} Question: Hello,
}
} Our lab obtained a Reichert Zetopan microscope (combined with Binolux III twin lamp unit). Unfortunately, we don't have any instruction/exploitation manuals both for the microscope and the unit.
} I have searched the archives of this site (http://www.microscopy.com) and found that others also had asked for such manuals. Some people had put materials in the web space but this sources are not available at present.
} Could anyone provide us with such documents (in electronic format or xeroxed; preferably in English)? If so, please, contact me at dax_uktc-at-abv.bg to discuss the details of submitting.
}
} Thanks in advance,
} Yordan N