Thanks to Roger, Steve Chapman and Bill Tivol for your replies - I do seem to remember knowing at one time that the objective and selected area apertures did opposite things when the objective lens is turned off, but it has been a while.. I did find I can get strong contrast in low mag (LM) mode using the SA aperture and have an acceptable field of view, although I couldn't get dislocations to be visible at 1000x in LM mode when they are blindingly obvious in standard mode at 2000x. Also a very significant increase in brightness if I turn C1 down below it's normal minimum using free lens control. Unfortunately, to see the dislocations I have to have a small aperture in the diffraction plane, so it's no use using larger apertures. So some more experimentation is needed, if I do get a good setup usilg FLC I'm happy to pass the information on to anyone else who finds they have the same problem.
-----Original Message----- X-from: Roger Ristau [mailto:raristau-at-ims.uconn.edu] Sent: 28 February 2007 16:37 To: Richard Beanland
Dear all,
Can anybody share their recent experience buying a new lab refrigerator? One of ours, a rather old machine, just broke, and we started looking up in the catalogs, so many choices there... Maybe someone has recently done the research and would be willing to share? I would appreciate specific comments.
This fridge is to be used for storing EM-related stuff, including antibodies, in the fridge and in the freezer compartment. I do realize, that people often want to avoid the models with auto defrost, because those actually warm up for some periods. But I used to think that putting the vials/tubes into one of those special Nalgene containers would protect the stuff from melting? Is that really true? Because if it is true, then it seems more convenient to get an auto one. Any experience on that?
Thanks! Vlad
________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource DBEPS/ORS, National Institutes of Health 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
==============================Original Headers============================== 7, 22 -- From vladislav_speransky-at-nih.gov Thu Mar 1 10:17:19 2007 7, 22 -- Received: from nihrelayxway.hub.nih.gov (nihrelayxway.hub.nih.gov [128.231.90.106]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l21GHJAF025752 7, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Mar 2007 10:17:19 -0600 7, 22 -- Received: from helix.nih.gov ([128.231.2.3]) 7, 22 -- by nihrelayxway.hub.nih.gov with ESMTP; 01 Mar 2007 11:17:19 -0500 7, 22 -- X-IronPortListener: NIH_Relay 7, 22 -- X-SBRS: None 7, 22 -- X-IronPort-AV: i="4.14,237,1170651600"; 7, 22 -- d="scan'208"; a="503510330:sNHT54953196" 7, 22 -- Received: from [156.40.102.124] ([156.40.102.124]) 7, 22 -- by helix.nih.gov (8.13.6/8.11.7/2SCANNER) with ESMTP id l21GHIOw50174634 7, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Mar 2007 11:17:18 -0500 (EST) 7, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- Message-Id: {CF3B016A-AE11-4A9D-9CD8-6DBF1220A2FB-at-nih.gov} 7, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 22 -- To: Microscopy-at-microscopy.com 7, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 7, 22 -- Subject: opinions wanted on lab refrigerator 7, 22 -- Date: Thu, 1 Mar 2007 11:15:52 -0500 7, 22 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
You do not want a standard refrigerator. It has to be explosion proof, which automatically rules out auto defrost. And no, the Nalge containers do not protect against the freeze/thaw cycle--particularly bad if you are storing any biologicals. One question--what temperature do you want/need your freezer to be? -10C? -20C? We just recently got a Barnstead with the -20C freezer. I think it's a monster, but it wasn't my decision.... There has been a long discussion about the negatives for auto defrost freezers on the histonet listserve. Google the listserve and search the archives. It was a lively and thorough discussion.
Roger Moretz, Ph.D. Dept of Toxicology Boehringer Ingelheim Pharmaceuticals Ridgefield, CT
On 3/1/07, vladislav_speransky-at-nih.gov {vladislav_speransky-at-nih.gov} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } } Can anybody share their recent experience buying a new lab } refrigerator? One of ours, a rather old machine, just broke, and we } started looking up in the catalogs, so many choices there... } Maybe someone has recently done the research and would be willing to } share? I would appreciate specific comments. } } This fridge is to be used for storing EM-related stuff, including } antibodies, in the fridge and in the freezer compartment. I do } realize, that people often want to avoid the models with auto } defrost, because those actually warm up for some periods. But I used } to think that putting the vials/tubes into one of those special } Nalgene containers would protect the stuff from melting? Is that } really true? Because if it is true, then it seems more convenient to } get an auto one. Any experience on that? } } Thanks! } Vlad } } } ________________________________________________ } Vlad Speransky, Staff Scientist } Supramolecular Structure and Function Resource } DBEPS/ORS, National Institutes of Health } 13 South Dr, Rm. 3N17 MSC 5766 } Bethesda, MD 20892 } 301 496-3989 } vladislav_speransky-at-nih.gov } } } ==============================Original Headers============================== } 7, 22 -- From vladislav_speransky-at-nih.gov Thu Mar 1 10:17:19 2007 } 7, 22 -- Received: from nihrelayxway.hub.nih.gov (nihrelayxway.hub.nih.gov [128.231.90.106]) } 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l21GHJAF025752 } 7, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Mar 2007 10:17:19 -0600 } 7, 22 -- Received: from helix.nih.gov ([128.231.2.3]) } 7, 22 -- by nihrelayxway.hub.nih.gov with ESMTP; 01 Mar 2007 11:17:19 -0500 } 7, 22 -- X-IronPortListener: NIH_Relay } 7, 22 -- X-SBRS: None } 7, 22 -- X-IronPort-AV: i="4.14,237,1170651600"; } 7, 22 -- d="scan'208"; a="503510330:sNHT54953196" } 7, 22 -- Received: from [156.40.102.124] ([156.40.102.124]) } 7, 22 -- by helix.nih.gov (8.13.6/8.11.7/2SCANNER) with ESMTP id l21GHIOw50174634 } 7, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Mar 2007 11:17:18 -0500 (EST) } 7, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) } 7, 22 -- Content-Transfer-Encoding: 7bit } 7, 22 -- Message-Id: {CF3B016A-AE11-4A9D-9CD8-6DBF1220A2FB-at-nih.gov} } 7, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed } 7, 22 -- To: Microscopy-at-microscopy.com } 7, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} } 7, 22 -- Subject: opinions wanted on lab refrigerator } 7, 22 -- Date: Thu, 1 Mar 2007 11:15:52 -0500 } 7, 22 -- X-Mailer: Apple Mail (2.752.2) } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 29 -- From rcmoretz-at-gmail.com Thu Mar 1 10:49:49 2007 4, 29 -- Received: from nf-out-0910.google.com (nf-out-0910.google.com [64.233.182.185]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l21GnmPn005405 4, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Mar 2007 10:49:49 -0600 4, 29 -- Received: by nf-out-0910.google.com with SMTP id a4so992045nfc 4, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 01 Mar 2007 08:49:48 -0800 (PST) 4, 29 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 4, 29 -- d=gmail.com; s=beta; 4, 29 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 4, 29 -- b=dXntUg6z4cE503+KsKEQBlTbHZE8Q2ByzXz8v5mF7ynIDjssD/7pUH8gwB+cr+Co5yM0TfTJS06hxcvPXAw1gplg3LuPOyMLRVkOwwK8Ravu66D8xMQAVJqYWr6IaXW+2yFxoYksz30DfzEKFSd92JTk0ehGc+1UFhmDnvg+RGI= 4, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 29 -- d=gmail.com; s=beta; 4, 29 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 4, 29 -- b=LNu51tm+jZhSPwHg2hAAGEqMxlk2ZUZALIvHte0yOG7OnavLIPWaGSVaRqhQJcRnGT0cVlhlKkNCKbmqtpYDnm5uARd/jMs2Ico1gkMHG6JKQozp07ZmvYO07a4s520sjTtZZIlxHj15Gtai0Gi52kcZmrrpQx3MG/97Rzignik= 4, 29 -- Received: by 10.82.167.5 with SMTP id p5mr654577bue.1172767787783; 4, 29 -- Thu, 01 Mar 2007 08:49:47 -0800 (PST) 4, 29 -- Received: by 10.82.167.20 with HTTP; Thu, 1 Mar 2007 08:49:47 -0800 (PST) 4, 29 -- Message-ID: {950e3cfd0703010849l5f71a650l79da171725fbc44a-at-mail.gmail.com} 4, 29 -- Date: Thu, 1 Mar 2007 11:49:47 -0500 4, 29 -- From: "Roger Moretz" {rcmoretz-at-gmail.com} 4, 29 -- To: vladislav_speransky-at-nih.gov, 4, 29 -- "Microscopy Listserv" {Microscopy-at-microscopy.com} 4, 29 -- Subject: Re: [Microscopy] opinions wanted on lab refrigerator 4, 29 -- In-Reply-To: {200703011624.l21GOk2h001675-at-ns.microscopy.com} 4, 29 -- MIME-Version: 1.0 4, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 29 -- Content-Transfer-Encoding: 7bit 4, 29 -- Content-Disposition: inline 4, 29 -- References: {200703011624.l21GOk2h001675-at-ns.microscopy.com} ==============================End of - Headers==============================
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Email: dennis1231-at-yahoo.com Name: Dennis McDaniel
Title-Subject: [Filtered] immunoEM antibody
Question: Hello,
I am doing some immunoEM work for another researcher, and so far none of the antibodies we have tried have been successful. The researcher would simply like to see the correct immunolocalization of anything in order to validate the fixation and labeling methods we are using.
I am in search of a commercially available antibody suitable for immunoEM in cells/tissues that have been fixed in 4% formaldehyde/0.5% glutaraldehyde. Ideally, the antibody would raised against an epitope that is present in most or all sections of most cells and that is localized to structures that may be easily discerned in cells that have not undergone optimal fixation (i.e. no OsO4 post-fix). I am thinking of something like an antibody to a mitochodrial or nuclear component. While I realize that no antibody will work in all cells, I was hoping someone may know of an antibody to a highly conserved protein that has worked in several different cell types.
First you should realize getting one antibody to work on a tissue fixed and embedded in a particular manner doesn't mean other antibodies will work in a similar fashion. Second, you fail to mention what species and tissue you are working on which makes suggestions of an antibody difficult. It is often easiest to look for a Medline or PubMed search of your tissue and species to see what antibodies have worked in EM immuno studies. Sometimes I just search the Journal of Histochemistry & Cytochemistry for a particular tissue since most of their papers do either LM or EM immuno. good luck.
At 11:00 AM 03/01/07, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
To assist you we really need more information. How are you attempting to perform the immunolabeling? Are you applying the antibodies to resin-embedded sections, cryosections or are you performing a pre-embedding protocol?
When you say that the labeling you are doing doesn't work, do you mean that the antibody is not labeling at all, is there too much label, or is the label in the "wrong" place?
There are a very large number of antibodies that do label tissues after been fixation using your fixative so it is easy to suspect that something is going on with your specimen preparation.
Try this: fix your tissues with formaldehyde alone and prepare the specimens for EM post-embedding (i.e. Labeling sections). Prepare semi-thin sections, mount them on glass coverslips (or slides) and immunolabel for light microscopy. You should be able to see a signal if you use fluorescent secondaries on LR White or Lowicryl-embedded material and cryosectioned specimens.
Contact me if you need more help.
Regards,
Paul Webster.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
} From: {dennis1231-at-yahoo.com} } Reply-To: {dennis1231-at-yahoo.com} } Date: Thu, 1 Mar 2007 11:03:42 -0600 } To: {pwebster-at-hei.org} } Subject: [Microscopy] viaWWW: immunoEM antibody } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both dennis1231-at-yahoo.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: dennis1231-at-yahoo.com } Name: Dennis McDaniel } } Title-Subject: [Filtered] immunoEM antibody } } Question: Hello, } } I am doing some immunoEM work for another researcher, and so far none } of the antibodies we have tried have been successful. The researcher } would simply like to see the correct immunolocalization of anything } in order to validate the fixation and labeling methods we are using. } } I am in search of a commercially available antibody suitable for } immunoEM in cells/tissues that have been fixed in 4% } formaldehyde/0.5% glutaraldehyde. Ideally, the antibody would raised } against an epitope that is present in most or all sections of most } cells and that is localized to structures that may be easily } discerned in cells that have not undergone optimal fixation (i.e. no } OsO4 post-fix). I am thinking of something like an antibody to a } mitochodrial or nuclear component. While I realize that no antibody } will work in all cells, I was hoping someone may know of an antibody } to a highly conserved protein that has worked in several different } cell types. } } Any suggestions? } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 12 -- From zaluzec-at-microscopy.com Thu Mar 1 11:00:04 2007 } 9, 12 -- Received: from [172.16.1.63] (msdvpn8.msd.anl.gov [130.202.238.72]) } 9, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l21H03xN017153 } 9, 12 -- for {microscopy-at-microscopy.com} ; Thu, 1 Mar 2007 11:00:03 -0600 } 9, 12 -- Mime-Version: 1.0 } 9, 12 -- X-Sender: (Unverified) } 9, 12 -- Message-Id: {p06020401c20cb8c68041-at-[172.16.1.63]} } 9, 12 -- Date: Thu, 1 Mar 2007 12:07:33 -0500 } 9, 12 -- To: microscopy-at-microscopy.com } 9, 12 -- From: dennis1231-at-yahoo.com (by way of MicroscopyListserver) } 9, 12 -- Subject: viaWWW: immunoEM antibody } 9, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
==============================Original Headers============================== 15, 20 -- From PWebster-at-hei.org Thu Mar 1 12:29:36 2007 15, 20 -- Received: from hi0sml1.hei.org (House-Ear-Institute-1114861.cust-rtr.pacbell.net [71.136.31.242]) 15, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l21ITaPB009259 15, 20 -- for {microscopy-at-microscopy.com} ; Thu, 1 Mar 2007 12:29:36 -0600 15, 20 -- Received: from 10.10.42.79 ([10.10.42.79]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 15, 20 -- Thu, 1 Mar 2007 18:29:35 +0000 15, 20 -- User-Agent: Microsoft-Entourage/11.3.3.061214 15, 20 -- Date: Thu, 01 Mar 2007 10:29:34 -0800 15, 20 -- Subject: Re: [Microscopy] viaWWW: immunoEM antibody 15, 20 -- From: "Webster, Paul" {PWebster-at-hei.org} 15, 20 -- To: {dennis1231-at-yahoo.com} 15, 20 -- CC: {microscopy-at-microscopy.com} 15, 20 -- Message-ID: {C20C5B8E.FA8A%PWebster-at-hei.org} 15, 20 -- Thread-Topic: [Microscopy] viaWWW: immunoEM antibody 15, 20 -- Thread-Index: AcdcL49BzZYFusgiEdui0AANk7Zh7g== 15, 20 -- In-Reply-To: {200703011703.l21H3gGW024034-at-ns.microscopy.com} 15, 20 -- Mime-version: 1.0 15, 20 -- Content-type: text/plain; 15, 20 -- charset="US-ASCII" 15, 20 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
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Email: sarj0007-at-unf.edu Name: Jason Saredy
Organization: University of North Florida
Title-Subject: [Filtered] Drying out live cells
Question: I have some cells in a MEM(mostly salts and amino acids and the like) that I would like to image with our ESEM in environmental mode. This is my first time trying to view live material and spent all afternoon trying to slowly dry out the sample and ended up over drying and lysing the cells. Does anyone with experience in this area know what would be the optimal way to slowly dry the sample in the chamber (ie what RH, how long, pressure)? Or perhaps a good reference on how to do this? Thank you. And I apologize if this went out three times, i tried emailing the listserv directly and it kept bouncing back.
A colleague is doing migration assays using transwell plates. The smaller transwells are darn near impossible to image because the wells are both too deep for an upright scope and not deep enough for an inverted. I suppose we could cut the membranes out of the wells and mount them on slides, but I seem to remember that they have the nasty habit of rolling up when you cut them out.
Does anyone have any good tricks/tips for imaging these membranes? Is there a mounting media with an RI that will make the membrane almost disappear?
Thanks, Doug
I asked what brand plates they were using and the response was:
-- ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Douglas W. Cromey, M.S. - Assistant Scientific Investigator Dept. of Cell Biology & Anatomy, University of Arizona 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
I am not 100% clear on your protocol but if you dry out cells in MEM, you are exposing them to a high osmotic buffer in the process and therefore it would be surprising if they didn't lyse. The water will evaporate but the salts simply concentrate.
At 04:50 PM 03/01/07, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
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Email: musselmannk-at-mail.nih.gov Name: Kurt Musselmann
Organization: NIH/NIDCR
Title-Subject: [Filtered] Fluorescence staining of frozen sections
Question: Hi all
I am trying to do fluorescence staining of frozen sections (10 um thick). Do I have to include a step in which I wash the OCT off the slides? or will fixing them in acetone do?
Alternatively, can I just fix the tissue in ethanol and proceed in the same fashion that I would for normal staining? (H&E, for example?). (ethanol, then wash with water to get rid of the OCT, then staining).
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Email: David.Llewellyn-at-anu.edu.au Name: David Llewellyn
Organization: Australian National University
Title-Subject: [Filtered] Equipment Wanted
Question: Would anyone have for sale any TEM Xsection prep. equipment? wanted to start up a new lab. Ion Beam mill,polishers,dimplers etc.
Hi John Thank you for that info. I did not know about turning off the printer to decrease the ink drying question. David
On Mar 2, 2007, at 10:00 AM, John Mackenzie wrote:
} } David: } } Yes and no. The C80 series is fairly stable but needs to be exercised } once a week. There are two things that are worth noting. The printer } retracts the ink when the printer is turned off USING THE BUTTON ON } THE } TOP. (Using a strip to kill power does not) If you leave the } printer on } all the time the ink is sitting at the ready and dries out faster. The } Epson system although the best does suffer from this problem. My } C80 was } more prone to this problem than the current C88. The C88 plus is so } fast } that it is worth the $80 to replace even a C88 let alone a C80. My } advice would be to use the ink you have then upgrade (For about the } cost } of inks you get new printer). It is REALLY fast. } } This info might be useful to others so if it is ok with you, you might } want to post it to the web. } } } } John } } } } David Elliott wrote: } } Hi John } } } } I have a question about inkjet printers. I have a C80 that does } } fine when it is used regularly, but if it is not used for a few } } days the ink jets clog up and much ink is wasted cleaning the } } print head. } } Is this the same for all inkjets? Is there something that I can } } do to keep my underused printers working? } } } } Thank you } } David } } } } } } On Feb 26, 2007, at 12:00 PM, john_mackenzie-at-ncsu.edu wrote: } } } } } } } } } } } } } } -------------------------------------------------------------------- } } } -------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society } } } of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } } MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } -------------------------------------------------------------------- } } } -------- } } } } } } Hi all: } } } } } } The needs of scientific digital imaging are not the same as they } } } are for } } } photography. We have done extensive testing of the printers and } } } it boils } } } down to two inkjets and one Laserjet } } } } } } The Epson C88 plus.. The best printer in the world for printing } } } on plain } } } paper C88 plus for a whopping $88.00 Nothing competes with it on } } } plain } } } paper. Total cost of a color print $0.17 with ink full coverage } } } } } } The best printer for black and white printing, and color } } } printing on } } } special papers is the Epson R2400. $700-800 range. } } } } } } The inks that Epson uses are very, very specialized and PATENTED. } } } All } } } "third party" inks are grossly inferior. ( Yes we have tested } } } several } } } but it requires purchasing flushing cartridges to switch so we } } } rarely do } } } it now) } } } } } } We really were impressed with the prints that John Cone was able to } } } produce.( Piezography B/W )They were striking HOWEVER the print } } } driver is seriously flawed } } } and will not give you results that are scientifically valid. The } } } new R2400 has } } } a dedicated K3 mode that beats the Cone system running away.It is } } } scientifically valid. I have both The Cone idea of using } } } multiple blacks is a } } } good one but Epsons implementation is the only one to use. Epson } } } has added } } } a B/W Tint mode that allows you to fine tune the BW so that it } } } looks just like } } } Agfa Boviera prints Longevity 200 years } } } } } } An 8 x 10 B/W print was $2 to $3 many years ago . If you actually } } } do the } } } experiment (which photographers do not) an 8 x 11 print on Premium } } } photo glossy paper with full coverage is $0.50 for the paper and } } } $0.15 } } } for the ink. If you use inferior ink with no tested longevity and } } } unknown fading characteristics you might save $0.10. This makes } } } no sense } } } to me.( We by the way use our darkroom to store inks and paper) } } } } } } We do most of our work prints on our HP Laserjet in high resolution } } } graphics mode (2400 dpi, 150 lpi ) at 2 cents a page (including } } } toner). } } } Also because the resolution is three times the advertised } } } resolution, } } } the you can't use refilled cartridges here either } } } } } } If you need a and a better print then use the c88. Save the best } } } for } } } the R2400. The three printers cost less than $2000. All } } } computers have } } } at least these three. } } } } } } In my workshops and my classes we spend 3-5 hours on this very } } } subject. } } } I know it is not simple and that this information is mainly the } } } bullet } } } conclusions. } } } } } } There are actually several other reasons for never using refilled } } } inks } } } or toners bottom line is that it is a bad practice and we never } } } do it. } } } We always use Epson papers for inkjets and we use Hammermill } } } Laser for } } } Lasrjets. (Sometimes Epson Bright white which costs the same) } } } } } } John } } } } } } --John Mackenzie, Jr. } } } Coordinator for the Center for Electron Microscopy } } } Professor of Microbiology } } } North Carolina State University } } } Phone (919) 515-2664 Fax (919) 515-8293 } } } } } } } } } } } } ==============================Original } } } Headers============================== } } } 15, 19 -- From john_mackenzie-at-ncsu.edu Mon Feb 26 12:56:34 2007 } } } 15, 19 -- Received: from uni05mr.unity.ncsu.edu } } } (uni05mr.unity.ncsu.edu [152.1.224.164]) } } } 15, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } } ESMTP id l1QIuXJ4002045 } } } 15, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Feb 2007 } } } 12:56:34 -0600 } } } 15, 19 -- Received: from [127.0.0.1] (cord1.mbio.ncsu.edu } } } [152.1.178.41]) } } } 15, 19 -- by uni05mr.unity.ncsu.edu (8.13.7/8.13.8/ } } } Nv5.2006.1109) with ESMTP id l1QIuWM1007166 } } } 15, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Feb 2007 } } } 13:56:33 -0500 (EST) } } } 15, 19 -- Message-ID: {45E32D65.9070004-at-ncsu.edu} } } } 15, 19 -- Date: Mon, 26 Feb 2007 13:56:37 -0500 } } } 15, 19 -- From: John Mackenzie {john_mackenzie-at-ncsu.edu} } } } 15, 19 -- User-Agent: Thunderbird 1.5.0.9 (Windows/20061207) } } } 15, 19 -- MIME-Version: 1.0 } } } 15, 19 -- To: Microscopy-at-microscopy.com } } } 15, 19 -- Subject: Re: Inkjet printers for EM, LM, Confocal, or } } } Scientific Photography } } } 15, 19 -- Content-Type: text/plain; charset=ISO-8859-1; } } } format=flowed } } } 15, 19 -- Content-Transfer-Encoding: 7bit } } } 15, 19 -- X-PMX-Version: 5.3.1.294258, Antispam-Engine: } } } 2.5.0.283055, Antispam-Data: 2007.2.26.104434 } } } 15, 19 -- X-Spam-Status: No, Hits=7% } } } 15, 19 -- X-Spam-Level: IIIIIII } } } ==============================End of - } } } Headers============================== } } } } -- } John M. Mackenzie Jr., PhD } Professor of Microbology } Coordinator, Center for Electron Microscopy } Phone 919-515-2664 Fax 919-515-8293 } }
==============================Original Headers============================== 5, 22 -- From Elliott-at-arizona.edu Fri Mar 2 11:31:17 2007 5, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.132.44]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l22HVHoq018215 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 2 Mar 2007 11:31:17 -0600 5, 22 -- Received: from gandalfs_amavis (amavis1.email.arizona.edu [10.0.0.204]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id D456912FB2 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 2 Mar 2007 10:31:16 -0700 (MST) 5, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id A8DD91200D 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 2 Mar 2007 10:31:12 -0700 (MST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 5, 22 -- In-Reply-To: {45E8583F.8040707-at-ncsu.edu} 5, 22 -- References: {200702261900.l1QJ0l3A018426-at-ns.microscopy.com} {B3EC61A2-85A7-49E5-A031-2AB04C1725DD-at-arizona.edu} {45E8583F.8040707-at-ncsu.edu} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {4C652ED0-293E-43C2-B961-3E7F413C31CE-at-arizona.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: David Elliott {Elliott-at-arizona.edu} 5, 22 -- Subject: Re: [Microscopy] Re: Inkjet printers for EM, LM, Confocal, or Scientific Photography 5, 22 -- Date: Fri, 2 Mar 2007 10:31:11 -0700 5, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 22 -- X-Mailer: Apple Mail (2.752.2) 5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
I know that Baltec has changed distribution in the last year or so. I was recently quoted a price for oscillator crystals of 2x what I had paid to the previous supplier. Fortunately I located some old stock (they were stored well, silver is not oxidized...) at the previous pricing. Double is quite a price jump.
Is there an alternative to these doubled prices? Does anyone using Baltec supplies have suggestions for supplies and support that haven't taken such a steep jump. We have a Freeze-Fracture and a turbo-pumped evaporator unit, and use the usual supplies for E-beam evaporators, film thickness monitors, and the like.
Thanks,
Dale Callaham
==============================Original Headers============================== 5, 19 -- From dac-at-research.umass.edu Fri Mar 2 12:11:18 2007 5, 19 -- Received: from race6.oit.umass.edu (race6.oit.umass.edu [128.119.101.44]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l22IBIGl030481 5, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 2 Mar 2007 12:11:18 -0600 5, 19 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 5, 19 -- (authenticated bits=0) 5, 19 -- by race6.oit.umass.edu (8.13.7/8.13.7) with ESMTP id l22IBHmZ029310 5, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 5, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 2 Mar 2007 13:11:18 -0500 5, 19 -- Message-ID: {45E86934.40705-at-research.umass.edu} 5, 19 -- Date: Fri, 02 Mar 2007 13:13:08 -0500 5, 19 -- From: Dale Callaham {dac-at-research.umass.edu} 5, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.2pre) Gecko/20070111 SeaMonkey/1.1 5, 19 -- MIME-Version: 1.0 5, 19 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 5, 19 -- Subject: Source of supplies for Balzers/Baltec (FF, evaporators) 5, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- X-Whitelist: TRUE ==============================End of - Headers==============================
We are looking at putting new video cameras on our teaching microscopes and would like to be able to use as much of the resolution of the data projectors in our classrooms as possible. In the past we've used small CCD NTSC cameras that were intended for security purposes, but now we'd like to be able to get something in the order of 1280 by 1024 pixels onto the screen.
We'd be very grateful for suggestions of what other users have found worked well for this purpose. Thanks for your thoughts. _____________________________________ Tom Gore | Advanced Imaging Laboratory Department of Biology | University of Victoria Box 3020 Station CSC Victoria BC V8W 3N5 Canada voice 250 721 7134 fax 250 721 7120 web: http://web.uvic.ca/ail/
==============================Original Headers============================== 3, 19 -- From togo-at-uvic.ca Fri Mar 2 13:19:36 2007 3, 19 -- Received: from castle.comp.uvic.ca (castle.comp.uvic.ca [142.104.5.97]) 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l22JJa4T010676 3, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 2 Mar 2007 13:19:36 -0600 3, 19 -- Received: from Amidol (amidol.biol.uvic.ca [142.104.208.15]) 3, 19 -- by castle.comp.uvic.ca (8.13.8/8.13.8) with ESMTP id l22JJHtk3252224 3, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 2 Mar 2007 11:19:17 -0800 3, 19 -- Message-Id: {4.2.0.58.20070302111549.03216880-at-pop.uvic.ca} 3, 19 -- X-Sender: togo-at-pop.uvic.ca 3, 19 -- X-Mailer: QUALCOMM Windows Eudora Pro Version 4.2.0.58 3, 19 -- Date: Fri, 02 Mar 2007 11:21:18 -0800 3, 19 -- To: Microscopy-at-microscopy.com 3, 19 -- From: Tom Gore {togo-at-uvic.ca} 3, 19 -- Subject: LM video cameras 3, 19 -- Mime-Version: 1.0 3, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 3, 19 -- X-UVic-Virus-Scanned: OK - Passed virus scan by Sophos (sophie) on castle 3, 19 -- X-UVic-Spam-Scan: castle.comp.uvic.ca Not_scanned_NO_SA 3, 19 -- X-Scanned-By: MIMEDefang 2.33 (www . roaringpenguin . com / mimedefang) ==============================End of - Headers==============================
Here is the March 2007 Microscopy Today table of contents. I will close the subscription list for this issue on Wednesday, Mar. 7th, 2007.
Microscopists in North America and MSA members anywhere can subscribe for free. Anyone else may subscribe for US$50 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com Thank you.
Ron Anderson, Editor ================================ Microscopy Reveals Early Neolithic Dentistry Stephen W. Carmichael, Mayo Clinic
Microscopic Analysis of Metal Recovered from the Wreck of RMS Titanic J.J. Hooper McCarty1 and T. Foecke1,2, 1The Johns Hopkins University, Baltimore, MD, 2NIST, Gaithersburg, MD
STEM Imaging of Lattice Fringes and beyond in a UHR In-Lens Field-Emission SEM Vinh Van Ngo, Mike Hernandez, Bill Roth, and David C Joy,* Hitachi High Technologies America, Inc., *University of Tennessee, Knoxville, TN
From the McArthur to the Millennium Health Microscope (MHM): Future Developments in Microscope Miniaturization for International Health Keith Dunning1 & J. Russell Stothard2, 1Dunning Associates, Bedford, UK., 2Natural History Museum, London, UK
A Novel Technique of Hair Removal to Examine the Cuticle of Arthropods B. N. Philip and C. Shillington, Eastern Michigan University, Ypsilanti, MI
Site Specific Three-dimensional Structural Analysis in Tissues and Cells Using Automated DualBeam Slice &View Ben Lich, FEI Company, Eindhoven, The Netherlands
Feature Characterization of Microfluidic Channels Created Using Direct Laser Ablation John Little* and Dan Borah,** *Hyphenated Systems, LLC, Burlingame, CA, **University of Mass., Dartmouth, MA
Negatice Stiffness Vibration Isolation Technology for Nanotechnology David L. Platus, Minus K Technology, Inc., Inglewood, CA
Improving Membrane Staining of Cultured Cells Using Ferrocyanide as a Post-Fixative Stéphane Nizet
The Preparation of Mg, Cd and Zn Samples for Crystal Orientation Mapping with BKD in an SEM R.A. Schwarzer, Clausthal Univ. of Technology, Clausthal-Zellerfeld, Germany
Visualisation of Native Surfaces by Two-Step Molding Stanislav N. Gorb, Max Planck Inst. for Metals Research, Stuttgart, Germany
Cold Temperature Preparation of XTEM Specimens of Embedded Metallic Nanoparticles Bernt Johannessen, David J. Llewellyn, Patrick Kluth, and Mark C. Ridgway, Australian National University, Canberra, Australia
Industry News NetNotes SAMPLE PREPARATION - frozen tissue for TEM SAMPLE PREPARATION - fixation of whole mosquitoes SAMPLE PREPARATION - fixation for mitochondria SAMPLE PREPARATION - fixation of mouse brain tissue SAMPLE PREPARATION - purpose of PVP SAMPLE PREPARATION - maleate buffers SAMPLE PREPARATION - Vibratome sections SAMPLE PREPARATION - LR White polymerization problem SAMPLE PREPARATION – SEM of cells without critical point drying SAMPLE PREPARATION - SEM of cells in monolayer SAMPLE PREPARATION - SEM of Zebra fish SAMPLE PREPARATION - critical point drying MICROTOMY - cryo-ultramicrotomy MICROTOMY - specimen advance LM - S waves in phase contrast optics LM - 3-D reconstruction from serial paraffin sections MICROSCOPY - LASIK, floaters, and posterior vitreous detachment TEM - defect density threshold TEM - effect of high temperature on grids TEM - cleaning a LaB6 emitter TEM - power line and stray field shielding SEM - solid state versus a scintillator SEM - low vs. high vacuum mode SEM - beam penetration EBL/ PMMA Mask
Index of Advertisers
==============================Original Headers============================== 18, 18 -- From microscopytoday-at-tampabay.rr.com Fri Mar 2 13:22:05 2007 18, 18 -- Received: from ms-smtp-03.tampabay.rr.com (ms-smtp-03.tampabay.rr.com [65.32.5.133]) 18, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l22JM5ox015372 18, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 2 Mar 2007 13:22:05 -0600 18, 18 -- Received: from [127.0.0.1] (rrcs-24-73-73-214.se.biz.rr.com [24.73.73.214]) 18, 18 -- by ms-smtp-03.tampabay.rr.com (8.13.6/8.13.6) with ESMTP id l22JM0eU012630; 18, 18 -- Fri, 2 Mar 2007 14:22:03 -0500 (EST) 18, 18 -- Message-ID: {45E87955.8010509-at-tampabay.rr.com} 18, 18 -- Date: Fri, 02 Mar 2007 14:21:57 -0500 18, 18 -- From: Microscopy Today {microscopytoday-at-tampabay.rr.com} 18, 18 -- User-Agent: Thunderbird 1.5.0.10 (Windows/20070221) 18, 18 -- MIME-Version: 1.0 18, 18 -- To: Listserver {Microscopy-at-Microscopy.Com} , 18, 18 -- Confocal Listserver {confocal-at-listserv.buffalo.edu} 18, 18 -- Subject: Microscopy Today Table of Contents March 2007 18, 18 -- Content-Type: text/plain; charset=windows-1252; format=flowed 18, 18 -- Content-Transfer-Encoding: 8bit 18, 18 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
If you find something like this, that would be interesting to know. The only ones I know of are not composite output but rather separate colors and sync and require a control box and special monitor (i.e., pricey).
By definition, NTSC is 768x494 and usually winds up being 640x480.
gary g.
At 11:21 AM 3/2/2007, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Fri Mar 2 14:08:55 2007 10, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l22K8tjj001834 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 2 Mar 2007 14:08:55 -0600 10, 20 -- Message-Id: {200703022008.l22K8tjj001834-at-ns.microscopy.com} 10, 20 -- Received: (qmail 19934 invoked from network); 2 Mar 2007 12:08:53 -0800 10, 20 -- Received: by simscan 1.1.0 ppid: 19931, pid: 19932, t: 0.1781s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp3 with SMTP; 2 Mar 2007 12:08:53 -0800 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Fri, 02 Mar 2007 12:06:50 -0800 10, 20 -- To: togo-at-uvic.ca 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] LM video cameras 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200703021921.l22JL8bf013579-at-ns.microscopy.com} 10, 20 -- References: {200703021921.l22JL8bf013579-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-549A7A2C ==============================End of - Headers==============================
I like the Jenoptik ProgRes series of cameras (http://www.jenoptik-los.de/cms.php?pageid=707&lang=1). Admittedly, I've never tried projecting my data but it looks good on the computer screen, especially with the CapturePro software that comes with the camera. We have the FireWire models and use them in materials research.
togo-at-uvic.ca wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We are looking at putting new video cameras on our teaching microscopes and } would like to be able to use as much of the resolution of the data } projectors in our classrooms as possible. In the past we've used small CCD } NTSC cameras that were intended for security purposes, but now we'd like } to be able to get something in the order of 1280 by 1024 pixels onto the } screen. } } We'd be very grateful for suggestions of what other users have found worked } well for this purpose. Thanks for your thoughts. } _____________________________________ } Tom Gore | Advanced Imaging Laboratory } Department of Biology | University of Victoria } Box 3020 Station CSC } Victoria BC V8W 3N5 Canada } voice 250 721 7134 fax 250 721 7120 } web: http://web.uvic.ca/ail/ } } } ==============================Original Headers============================== } 3, 19 -- From togo-at-uvic.ca Fri Mar 2 13:19:36 2007 } 3, 19 -- Received: from castle.comp.uvic.ca (castle.comp.uvic.ca [142.104.5.97]) } 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l22JJa4T010676 } 3, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 2 Mar 2007 13:19:36 -0600 } 3, 19 -- Received: from Amidol (amidol.biol.uvic.ca [142.104.208.15]) } 3, 19 -- by castle.comp.uvic.ca (8.13.8/8.13.8) with ESMTP id l22JJHtk3252224 } 3, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 2 Mar 2007 11:19:17 -0800 } 3, 19 -- Message-Id: {4.2.0.58.20070302111549.03216880-at-pop.uvic.ca} } 3, 19 -- X-Sender: togo-at-pop.uvic.ca } 3, 19 -- X-Mailer: QUALCOMM Windows Eudora Pro Version 4.2.0.58 } 3, 19 -- Date: Fri, 02 Mar 2007 11:21:18 -0800 } 3, 19 -- To: Microscopy-at-microscopy.com } 3, 19 -- From: Tom Gore {togo-at-uvic.ca} } 3, 19 -- Subject: LM video cameras } 3, 19 -- Mime-Version: 1.0 } 3, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } 3, 19 -- X-UVic-Virus-Scanned: OK - Passed virus scan by Sophos (sophie) on castle } 3, 19 -- X-UVic-Spam-Scan: castle.comp.uvic.ca Not_scanned_NO_SA } 3, 19 -- X-Scanned-By: MIMEDefang 2.33 (www . roaringpenguin . com / mimedefang) } ==============================End of - Headers============================== } }
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 4, 23 -- From r-holdford-at-ti.com Fri Mar 2 14:53:18 2007 4, 23 -- Received: from soda.ext.ti.com (soda.ext.ti.com [198.47.26.145]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l22KrIhA014141 4, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 2 Mar 2007 14:53:18 -0600 4, 23 -- Received: from dlep34.itg.ti.com ([157.170.170.115]) 4, 23 -- by soda.ext.ti.com (8.13.7/8.13.7) with ESMTP id l22Kqlah022844 4, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 4, 23 -- Fri, 2 Mar 2007 14:52:58 -0600 4, 23 -- Received: from [156.117.194.120] (localhost [127.0.0.1]) 4, 23 -- by dlep34.itg.ti.com (8.13.7/8.13.7) with ESMTP id l22KqgSZ005306; 4, 23 -- Fri, 2 Mar 2007 14:52:42 -0600 (CST) 4, 23 -- Message-ID: {45E88E99.10601-at-ti.com} 4, 23 -- Date: Fri, 02 Mar 2007 14:52:41 -0600 4, 23 -- From: Becky Holdford {r-holdford-at-ti.com} 4, 23 -- Organization: SC Packaging Development -- FA Development 4, 23 -- User-Agent: Thunderbird 1.5.0.10 (Windows/20070221) 4, 23 -- MIME-Version: 1.0 4, 23 -- To: togo-at-uvic.ca, MSA ListServer {Microscopy-at-MSA.Microscopy.Com} 4, 23 -- Subject: Re: [Microscopy] LM video cameras 4, 23 -- References: {200703021919.l22JJoe4010984-at-ns.microscopy.com} 4, 23 -- In-Reply-To: {200703021919.l22JJoe4010984-at-ns.microscopy.com} 4, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
By some happy chance, might somebody in Listland have surplus film drying cabinet? We are making room for a new microscope and need to get rid of our BIG film dryer and hope to replace it with one of a size more suited to the new world of digital micrography----i.e., much smaller.
We would be happy to pay shipping and a reasonable price for a dust-discouraging dryer, ideally with a fan.
Thanks, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 6, 23 -- From TindallR-at-missouri.edu Fri Mar 2 15:17:07 2007 6, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l22LH70q025606 6, 23 -- for {microscopy-at-microscopy.com} ; Fri, 2 Mar 2007 15:17:07 -0600 6, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 6, 23 -- Fri, 2 Mar 2007 15:17:06 -0600 6, 23 -- x-mimeole: Produced By Microsoft Exchange V6.5 6, 23 -- Content-class: urn:content-classes:message 6, 23 -- MIME-Version: 1.0 6, 23 -- Content-Type: text/plain; 6, 23 -- charset="us-ascii" 6, 23 -- Subject: Dryer for TEM films? 6, 23 -- Date: Fri, 2 Mar 2007 15:17:06 -0600 6, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A41C68E8D-at-UM-XMAIL08.um.umsystem.edu} 6, 23 -- X-MS-Has-Attach: 6, 23 -- X-MS-TNEF-Correlator: 6, 23 -- Thread-Topic: Dryer for TEM films? 6, 23 -- Thread-Index: AcddECF6/HGLumWARk2WxLhOrDI7sw== 6, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 6, 23 -- To: {microscopy-at-microscopy.com} 6, 23 -- X-OriginalArrivalTime: 02 Mar 2007 21:17:07.0047 (UTC) FILETIME=[21C07770:01C75D10] 6, 23 -- Content-Transfer-Encoding: 8bit 6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l22LH70q025606 ==============================End of - Headers==============================
Dear Tom, I just purchased a Motic (www.motic.com) microscope camera to replace the old Pixera camera that died. These are not video cameras, but frame capture cameras that capture a 1.3 megapixel colour image directly into a computer over about 10 seconds. The focus mode is a faster scan, but not as high resolution. The whole kit was only $430 CAD and included about five adapters to fit the camera to the light microscope, all cables and software. I think you will have a problem finding a high-resolution video camera and it might be very expensive. Having said that, you can now purchase a consumer HD video camera for less than $1000. If it has a live output to the computer and you can make an adaptor to fit it to the microscope, it should work. If you don't really need video capture speeds, you will get more for less money with a frame capture system. My two pixels worth. Regards,
-----Original Message----- X-from: togo-at-uvic.ca [mailto:togo-at-uvic.ca] Sent: March 2, 2007 11:25 AM To: mager-at-interchange.ubc.ca
We are looking at putting new video cameras on our teaching microscopes and would like to be able to use as much of the resolution of the data projectors in our classrooms as possible. In the past we've used small CCD NTSC cameras that were intended for security purposes, but now we'd like to be able to get something in the order of 1280 by 1024 pixels onto the screen.
We'd be very grateful for suggestions of what other users have found worked well for this purpose. Thanks for your thoughts. _____________________________________ Tom Gore | Advanced Imaging Laboratory Department of Biology | University of Victoria Box 3020 Station CSC Victoria BC V8W 3N5 Canada voice 250 721 7134 fax 250 721 7120 web: http://web.uvic.ca/ail/
==============================Original Headers============================== 3, 19 -- From togo-at-uvic.ca Fri Mar 2 13:19:36 2007 3, 19 -- Received: from castle.comp.uvic.ca (castle.comp.uvic.ca [142.104.5.97]) 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l22JJa4T010676 3, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 2 Mar 2007 13:19:36 -0600 3, 19 -- Received: from Amidol (amidol.biol.uvic.ca [142.104.208.15]) 3, 19 -- by castle.comp.uvic.ca (8.13.8/8.13.8) with ESMTP id l22JJHtk3252224 3, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 2 Mar 2007 11:19:17 -0800 3, 19 -- Message-Id: {4.2.0.58.20070302111549.03216880-at-pop.uvic.ca} 3, 19 -- X-Sender: togo-at-pop.uvic.ca 3, 19 -- X-Mailer: QUALCOMM Windows Eudora Pro Version 4.2.0.58 3, 19 -- Date: Fri, 02 Mar 2007 11:21:18 -0800 3, 19 -- To: Microscopy-at-microscopy.com 3, 19 -- From: Tom Gore {togo-at-uvic.ca} 3, 19 -- Subject: LM video cameras 3, 19 -- Mime-Version: 1.0 3, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 3, 19 -- X-UVic-Virus-Scanned: OK - Passed virus scan by Sophos (sophie) on castle 3, 19 -- X-UVic-Spam-Scan: castle.comp.uvic.ca Not_scanned_NO_SA 3, 19 -- X-Scanned-By: MIMEDefang 2.33 (www . roaringpenguin . com / mimedefang) ==============================End of - Headers==============================
==============================Original Headers============================== 11, 33 -- From mager-at-interchange.ubc.ca Fri Mar 2 15:54:36 2007 11, 33 -- Received: from mta3.mail-relay.ubc.ca (mta3.mail-relay.ubc.ca [137.82.45.6]) 11, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l22LsaZe005106 11, 33 -- for {microscopy-at-microscopy.com} ; Fri, 2 Mar 2007 15:54:36 -0600 11, 33 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 11, 33 -- by mta3.mail-relay.ubc.ca (8.12.11.20060308/8.12.11) with ESMTP id l22LsZTt019853 11, 33 -- for {microscopy-at-microscopy.com} ; Fri, 2 Mar 2007 13:54:35 -0800 (PST) 11, 33 -- (envelope-from mager-at-interchange.ubc.ca) 11, 33 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 11, 33 -- by smtp.interchange.ubc.ca 11, 33 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 11, 33 -- with ESMTPS id {0JEA00INJQ6Z53-at-smtp.interchange.ubc.ca} for 11, 33 -- microscopy-at-microscopy.com; Fri, 02 Mar 2007 13:54:35 -0800 (PST) 11, 33 -- Date: Fri, 02 Mar 2007 13:54:34 -0800 11, 33 -- From: Mary Mager {mager-at-interchange.ubc.ca} 11, 33 -- Subject: RE: [Microscopy] LM video cameras 11, 33 -- In-reply-to: {200703021925.l22JPBCv025819-at-ns.microscopy.com} 11, 33 -- To: togo-at-uvic.ca 11, 33 -- Cc: "'Microscopy'" {microscopy-at-microscopy.com} 11, 33 -- Reply-to: mager-at-interchange.ubc.ca 11, 33 -- Message-id: {0JEA00INKQ6Z53-at-smtp.interchange.ubc.ca} 11, 33 -- Organization: Materials Eng. UBC 11, 33 -- MIME-version: 1.0 11, 33 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 11, 33 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 11, 33 -- Content-type: text/plain; charset=us-ascii 11, 33 -- Content-transfer-encoding: 7bit 11, 33 -- Thread-index: AcddAH9FZwlsfb61RAOkGtnQVruF/gAAcbIA 11, 33 -- X-UBC-Scanned: Sophos PureMessage 4.7.1.128075, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.3.2.123934 11, 33 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 11, 33 -- X-PerlMx-Spam: Probability=7%, Report=__C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_LOC 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_PHRASE3 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __USER_AGENT_MS_GENERIC 0 11, 33 -- X-Spam-Level: 11, 33 -- X-Spam-Flag: No ==============================End of - Headers==============================
I have an ISI ABT-SX40A SEM EBIC module, including the following:
EMF-II IE Amp (Unit from inside the chamber)
NIIOREOIP option board (Controller for the EBIC Image contrast)
connecting cables to connect into an AMS-110 option box.
All was working when microscope was decomissioned. I will include the control module box if necessary, but if you don't need it, let me know.
I also have an extra Denton Desk II Carbon Accessory. I don't have the sputter coater, so this is extra to my needs.
I have no use for these, but if you would like them, let me know what you have to trade.
I run a non-profit organization which allows middle and high school students to submit research proposals and complete research using our lab facility.
I would really like some kind of digital imaging system (Any kind that will work on an older analog SEM), backscattered electron detector, or a sputter coater.
Thanks,
Justin A. Kraft Director Center for Inquiry-Based Science Education
==============================Original Headers============================== 12, 26 -- From kraftpiano-at-gmail.com Fri Mar 2 17:10:38 2007 12, 26 -- Received: from ug-out-1314.google.com (ug-out-1314.google.com [66.249.92.169]) 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l22NAcOW018000 12, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 2 Mar 2007 17:10:38 -0600 12, 26 -- Received: by ug-out-1314.google.com with SMTP id m2so822937ugc 12, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 02 Mar 2007 15:10:37 -0800 (PST) 12, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 12, 26 -- d=gmail.com; s=beta; 12, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 12, 26 -- b=SFP2GxMvsDbVtr1xb3xdafig0IiEgz5E2lO30IkH9yk38wUKRUmn4946ovy1xXDv9YY+lu34qxCL3qJO+GUFGoP2WSBbmQzzPpIpT45TRAt+hqpLCWa1uh6bmPMnQb8oTOqvfya1PKaLF5+AVY6yX1yJvLhp3mVUFic3A82KO84= 12, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 12, 26 -- d=gmail.com; s=beta; 12, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 12, 26 -- b=D6L1vmkdhzPsSRXq3aozyS13Sc2k/K/eiTDcQOqYejHEoMPL1IOb+Olk6YAapBYx4o0It6cDdYsEXdC3zs5vxtB1XDIxfS+j32Vu48Z/gO9+LW00cuqpL4NNumq1k7BM9h5QM0aGbv/4fnhLAu4HWGYEGTdi3uPSsBoEtIkF+cM= 12, 26 -- Received: by 10.115.108.1 with SMTP id k1mr435305wam.1172877036997; 12, 26 -- Fri, 02 Mar 2007 15:10:36 -0800 (PST) 12, 26 -- Received: by 10.114.79.12 with HTTP; Fri, 2 Mar 2007 15:10:36 -0800 (PST) 12, 26 -- Message-ID: {25e2b0d20703021510u119ef2bh58f3ed315c047181-at-mail.gmail.com} 12, 26 -- Date: Fri, 2 Mar 2007 18:10:36 -0500 12, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 12, 26 -- To: Microscopy-at-microscopy.com 12, 26 -- Subject: SEM EBIC module & Carbon accessory extra to my needs. Trade? 12, 26 -- MIME-Version: 1.0 12, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 26 -- Content-Transfer-Encoding: 7bit 12, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
} ASSISTANT PROFESSOR – MICROBIOLOGY AND CELL SCIENCE } } and } } DIRECTOR of ELECTRON MICROSCOPY and BIOIMAGING LABORATORY in the INTERDISCIPLINARY CENTER FOR BIOTECHNOLOGY RESEARCH (ICBR) } } } The Department of Microbiology and Cell Science, in partnership with the Interdisciplinary Center for Biotechnology Research (ICBR) at the University of Florida, is seeking a dynamic individual for a tenure accruing faculty position and the head of an electron microscopy and bioimaging core laboratory. } } The successful candidate will be expected to develop and maintain a strong, externally-funded research program in microbiology or cell science, participate in teaching, and direct the Electron Microscopy and Bioimaging Laboratory (EMBL) within UF’s ICBR, Technical support staff will be provided to perform the daily service tasks of this ICBR research facility. As the director, the incumbent will be charged with setting the vision and acquiring the resources to make the ICBR EMBL among the best in the nation. Candidates with interests in applying modern bioimaging technology, including electron microscopy, to important problems in cellular or molecular biology are especially encouraged to apply. } } } This is a 12-month tenure-accruing position that has 50% research, 25% service, and 25% instructional components. This assignment may change in accordance with the needs of the units. Instructional responsibilities will be to participate in teaching undergraduate and graduate courses. The faculty member will also supervise graduate student theses and dissertations. } } } Required qualifications are a Ph.D. degree in cell biology or related field, at least two years of postdoctoral research experience, and a significant record of productivity, as demonstrated through refereed publications, including published productivity in bioimaging. } } Salary and startup package will be competitive. } } } To apply, submit a cover letter, curriculum vitae, a statement of research and teaching interests in one electronic file to the email below. Request official transcripts of your graduate academic work to be sent directly from institution to the address below. Also request that 3 referees send letters of recommendation to the email below and a signed copy of the letter to the address below. Review of applications will begin March 15, 2007. Nominations of candidates are encouraged. Women and minorities are encouraged to apply. Please forward all applications, nominations and inquiries to: } } } Dr. Peter Kima } } Chair, Search and Screen Committee } } Department of Microbiology and Cell Science } } University of Florida, PO Box 110700, Gainesville, FL 32611-0700 } } Phone: (352)392-0384; Fax: (352)392-5922. E-mail: pkima-at-ufl.edu } } } BACKGROUND INFORMATION: The Department of Microbiology and Cell Science is an academic unit in the College of Agricultural and Life Sciences within the Institute of Food and Agricultural Sciences (IFAS) of the University of Florida (http://microcell.ufl.edu). It has well established and extramurally funded programs in the areas of immunology, microbial genetics, biochemistry, and physiology, as well as host-parasite relationships, innate immunity, and biotechnology. The Department offers B.S., M.S. and Ph.D. degrees through the College of Agricultural and Life Sciences; the undergraduate degree is also offered through the College of Liberal Arts and Sciences. Research programs are sponsored through the Florida Agricultural Experiment Station. The University of Florida, a land-grant university and member of the Association of American Universities, enrolls approximately 48,000 students in seventeen academic units, including the Colleges of Agricultural and Life Sciences, Dentistry, Engineering, Liberal Arts and Sciences, Medicine, and Veterinary Medicine, and the School Of Natural Resources and Environment. } } } The ICBR is a campus-wide center that provides state-of-the-art scientific expertise, training, instrumentation, and technologies to faculty, staff, graduate students, and other research partners throughout the university, state and nation (www.biotech.ufl.edu). The ICBR Core Laboratories, including the Electron Microscopy Bioimaging Laboratory, are staffed by ICBR personnel who are expert in each of the research laboratory technologies (genomics, proteomics, sequencing, gene expression, mass spectrometry, microarrays, hybridoma, flow cytometry, electron microscopy, molecular biomarkers, genetic analysis, and bioinformatics). The staff also advises and offers scientific and technical consultation for investigators, and is strongly committed to developing new advances in their technology areas and to passing this knowledge on to faculty users. } } The University is located in Gainesville, Florida, a community with a population of approximately 150,000 people in the metropolitan area, located in North Central Florida, approximately mid-way between the Atlantic Ocean and the Gulf of Mexico. }
-- Greg Erdos University of Florida, Retired Micanopy, Florida
==============================Original Headers============================== 3, 23 -- From gwe-at-ufl.edu Sat Mar 3 17:57:05 2007 3, 23 -- Received: from smtp.ufl.edu (smtp02.osg.ufl.edu [128.227.74.165]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l23Nv4BF025660 3, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sat, 3 Mar 2007 17:57:05 -0600 3, 23 -- Received: from [10.228.0.2] (ssrb-vpn1-0-2.vpn.ufl.edu [10.228.0.2]) 3, 23 -- (authenticated bits=0) 3, 23 -- by smtp.ufl.edu (8.13.7/8.13.7/2.5.9) with ESMTP id l23NuxSH3973160 3, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sat, 3 Mar 2007 18:57:01 -0500 3, 23 -- Message-ID: {45EA0B58.3000707-at-ufl.edu} 3, 23 -- Date: Sat, 03 Mar 2007 18:57:12 -0500 3, 23 -- From: Greg Erdos {gwe-at-ufl.edu} 3, 23 -- Reply-To: gwe-at-ufl.edu 3, 23 -- User-Agent: Thunderbird 1.5.0.10 (Windows/20070221) 3, 23 -- MIME-Version: 1.0 3, 23 -- To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com} 3, 23 -- Subject: Microsopy Position 3, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 3, 23 -- Content-Transfer-Encoding: 8bit 3, 23 -- X-Greylist: Sender succeeded SMTP AUTH authentication, not delayed by milter-greylist-3.0rc3 (smtp.ufl.edu [128.227.74.56]); Sat, 03 Mar 2007 18:57:03 -0500 (EST) 3, 23 -- X-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 3, 23 -- X-UFL-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 3, 23 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 3, 23 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
A friend of mine needs a picture of a mosquito by SEM to illustrate a presentation about mosquitoes. Does anyone knows where to find good quality pictures for free?
Best regards,
Stephane
____________________________________________________________________________________ Get your own web address. Have a HUGE year through Yahoo! Small Business. http://smallbusiness.yahoo.com/domains/?p=BESTDEAL
==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Sun Mar 4 13:33:37 2007 6, 19 -- Received: from web37415.mail.mud.yahoo.com (web37415.mail.mud.yahoo.com [209.191.91.147]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l24JXaoa004562 6, 19 -- for {microscopy-at-microscopy.com} ; Sun, 4 Mar 2007 13:33:36 -0600 6, 19 -- Received: (qmail 51090 invoked by uid 60001); 4 Mar 2007 19:33:48 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 6, 19 -- b=FqZJEHm63q0QA5xJ/BJ0IQHhBPT4ngOhZoRz+6p0TxeJzICJIy2iSqoRH5bsY1X69HdMlxiIGwEV6/gR1ES3iNId0lTpTf8GTfiWL9lStMv8pj2ZRzDh4K+uSdjjwDXgJv/J0wCVTQN3xlFbH6GuwS0ro9hpCgwRMpK5zxWZM5I=; 6, 19 -- X-YMail-OSG: I8j9VwIVM1nmzRq49U_93L2pHUKGCEcxckLJjPyal4UcVJxl8kcmzXLi_hzK1v0AKM_1l37AlD1Pj697tUXUj0WN9YKl2do3HYiVP4mTbVrBP2tzHD5jUHj_qfGFcoKQp5KFA2YAuiVZiJ8- 6, 19 -- Received: from [80.121.15.13] by web37415.mail.mud.yahoo.com via HTTP; Sun, 04 Mar 2007 11:33:48 PST 6, 19 -- Date: Sun, 4 Mar 2007 11:33:48 -0800 (PST) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 -- Subject: mosquito by SEM 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- Message-ID: {643889.51079.qm-at-web37415.mail.mud.yahoo.com} ==============================End of - Headers==============================
Alternatively, you can do a Google image search to see what happens.
Good luck.
David Henriks Henriks-at-cox.net
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Sunday, March 04, 2007 11:43 AM To: Henriks-at-cox.net
Dear colleagues,
A friend of mine needs a picture of a mosquito by SEM to illustrate a presentation about mosquitoes. Does anyone knows where to find good quality pictures for free?
Best regards,
Stephane
____________________________________________________________________________ ________ Get your own web address. Have a HUGE year through Yahoo! Small Business. http://smallbusiness.yahoo.com/domains/?p=BESTDEAL
==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Sun Mar 4 13:33:37 2007 6, 19 -- Received: from web37415.mail.mud.yahoo.com (web37415.mail.mud.yahoo.com [209.191.91.147]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l24JXaoa004562 6, 19 -- for {microscopy-at-microscopy.com} ; Sun, 4 Mar 2007 13:33:36 -0600 6, 19 -- Received: (qmail 51090 invoked by uid 60001); 4 Mar 2007 19:33:48 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Conten t-Transfer-Encoding:Message-ID; 6, 19 -- b=FqZJEHm63q0QA5xJ/BJ0IQHhBPT4ngOhZoRz+6p0TxeJzICJIy2iSqoRH5bsY1X69HdMlxiIGw EV6/gR1ES3iNId0lTpTf8GTfiWL9lStMv8pj2ZRzDh4K+uSdjjwDXgJv/J0wCVTQN3xlFbH6GuwS 0ro9hpCgwRMpK5zxWZM5I=; 6, 19 -- X-YMail-OSG: I8j9VwIVM1nmzRq49U_93L2pHUKGCEcxckLJjPyal4UcVJxl8kcmzXLi_hzK1v0AKM_1l37AlD1P j697tUXUj0WN9YKl2do3HYiVP4mTbVrBP2tzHD5jUHj_qfGFcoKQp5KFA2YAuiVZiJ8- 6, 19 -- Received: from [80.121.15.13] by web37415.mail.mud.yahoo.com via HTTP; Sun, 04 Mar 2007 11:33:48 PST 6, 19 -- Date: Sun, 4 Mar 2007 11:33:48 -0800 (PST) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 -- Subject: mosquito by SEM 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- Message-ID: {643889.51079.qm-at-web37415.mail.mud.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 17, 26 -- From henriks-at-cox.net Sun Mar 4 14:02:32 2007 17, 26 -- Received: from fed1rmmtao102.cox.net (fed1rmmtao102.cox.net [68.230.241.44]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l24K2WLd016148 17, 26 -- for {microscopy-at-microscopy.com} ; Sun, 4 Mar 2007 14:02:32 -0600 17, 26 -- Received: from fed1rmimpo02.cox.net ([70.169.32.72]) 17, 26 -- by fed1rmmtao102.cox.net 17, 26 -- (InterMail vM.7.05.02.00 201-2174-114-20060621) with ESMTP 17, 26 -- id {20070304200243.BGNG26279.fed1rmmtao102.cox.net-at-fed1rmimpo02.cox.net} ; 17, 26 -- Sun, 4 Mar 2007 15:02:43 -0500 17, 26 -- Received: from DellE310 ([70.181.99.26]) 17, 26 -- by fed1rmimpo02.cox.net with bizsmtp 17, 26 -- id Wk2e1W00h0a9jum0000000; Sun, 04 Mar 2007 15:02:44 -0500 17, 26 -- From: "David Henriks" {henriks-at-cox.net} 17, 26 -- To: {nizets2-at-yahoo.com} 17, 26 -- Cc: {microscopy-at-microscopy.com} 17, 26 -- Subject: RE: [Microscopy] mosquito by SEM 17, 26 -- Date: Sun, 4 Mar 2007 12:01:06 -0800 17, 26 -- Message-ID: {01c101c75e97$d9040360$3c01a8c0-at-DellE310} 17, 26 -- MIME-Version: 1.0 17, 26 -- Content-Type: text/plain; 17, 26 -- charset="us-ascii" 17, 26 -- Content-Transfer-Encoding: 7bit 17, 26 -- X-Mailer: Microsoft Office Outlook 11 17, 26 -- Thread-Index: AcdelVkFy75k6veRTjaZi+o47rALMQAAknKQ 17, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 17, 26 -- In-Reply-To: AAAAAMY7gB18BfNPgPDRzX6JX+Hk3iMA ==============================End of - Headers==============================
Well, I don't think my weekend would have been complete without the "wisdom" and nasty-toned comments of Mr. Smith. As a simple technician trying to hold the line on costs for our clients, I think of information exchange via the web as a very good basis for letting the "free-market" operate. It worked wonders in remote villages of India where access to telephones allowed poor farmers to get proper competitive prices for their crops. And maybe I'm really out of touch, but salaries and benefits in my neighborhood have been doing a lot of things BESIDES keeping up.
I wish JERRY SMITH well in his search for full actualization using both UPPER and lower case character coding.......
Dale Callaham
JERRY SMITH wrote: } } ----- Original Message ----- From: "JERRY SMITH" {JSMIT51-at-tampabay.rr.com} } To: "JERRY SMITH" {JSMIT51-at-tampabay.rr.com} } Sent: Saturday, March 03, 2007 10:32 AM } Subject: Re: [Microscopy] Source of supplies for Balzers/Baltec (FF, } evaporators) } } } } BUT SUPPLIES HAVE ALWAYS BEEN KIND OF ERRATICALLY PRICED, SOMETIMES NOT } } INCREASING FOR 6 YEARS AND THEN GOING CONSIDERABLY HIGHER, I GUESS } } THAT IS THE FREE MARKET SYSTEM } } ----- Original Message ----- From: "JERRY SMITH" } } {JSMIT51-at-tampabay.rr.com} } } To: {dac-at-research.umass.edu} } } Sent: Saturday, March 03, 2007 10:26 AM } } Subject: Re: [Microscopy] Source of supplies for Balzers/Baltec (FF, } } evaporators) } } } } } } } I GUESS THEY THINK ONLY THEIR SALARIES } } } AND BENEFITS GO UP AND EVERYONE } } } ELSE SHOULD STICK TO THE OLD PRICE FOR A HUNDRED YEARS } } } ----- Original Message ----- From: {dac-at-research.umass.edu} } } } To: {JSMIT51-at-TAMPABAY.RR.COM} } } } Sent: Friday, March 02, 2007 1:12 PM } } } Subject: [Microscopy] Source of supplies for Balzers/Baltec (FF, } } } evaporators) } } } } } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } } } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } } America } } } } To Subscribe/Unsubscribe -- } } } } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } ---------------------------------------------------------------------------- } } } } } } } } } } } } Hi all, } } } } } } } } I know that Baltec has changed distribution in the last year or so. I } } } } was recently quoted a price for oscillator crystals of 2x what I had } } } } paid to the previous supplier. Fortunately I located some old stock } } } } (they were stored well, silver is not oxidized...) at the previous } } } } pricing. Double is quite a price jump. } } } } } } } } Is there an alternative to these doubled prices? Does anyone using } } } } Baltec supplies have suggestions for supplies and support that haven't } } } } taken such a steep jump. We have a Freeze-Fracture and a turbo-pumped } } } } evaporator unit, and use the usual supplies for E-beam evaporators, } } } } film } } } } thickness monitors, and the like. } } } } } } } } Thanks, } } } } } } } } Dale Callaham } } } } } } } } ==============================Original } } } } Headers============================== } } } } 5, 19 -- From dac-at-research.umass.edu Fri Mar 2 12:11:18 2007 } } } } 5, 19 -- Received: from race6.oit.umass.edu (race6.oit.umass.edu } } } } [128.119.101.44]) } } } } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } } } id l22IBIGl030481 } } } } 5, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 2 Mar 2007 12:11:18 } } } } -0600 } } } } 5, 19 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu } } } } [128.119.55.30]) } } } } 5, 19 -- (authenticated bits=0) } } } } 5, 19 -- by race6.oit.umass.edu (8.13.7/8.13.7) with ESMTP id } } } } l22IBHmZ029310 } } } } 5, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 } } } } verify=NOT) } } } } 5, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 2 Mar 2007 13:11:18 } } } } -0500 } } } } 5, 19 -- Message-ID: {45E86934.40705-at-research.umass.edu} } } } } 5, 19 -- Date: Fri, 02 Mar 2007 13:13:08 -0500 } } } } 5, 19 -- From: Dale Callaham {dac-at-research.umass.edu} } } } } 5, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; } } } } rv:1.8.1.2pre) Gecko/20070111 SeaMonkey/1.1 } } } } 5, 19 -- MIME-Version: 1.0 } } } } 5, 19 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} } } } } 5, 19 -- Subject: Source of supplies for Balzers/Baltec (FF, } } } } evaporators) } } } } 5, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } } } 5, 19 -- Content-Transfer-Encoding: 7bit } } } } 5, 19 -- X-Whitelist: TRUE } } } } ==============================End of - } } } } Headers============================== } } } } } } } } } } }
==============================Original Headers============================== 4, 20 -- From dac-at-research.umass.edu Sun Mar 4 22:05:56 2007 4, 20 -- Received: from race6.oit.umass.edu (race6.oit.umass.edu [128.119.101.44]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2545u7s006167 4, 20 -- for {microscopy-at-microscopy.com} ; Sun, 4 Mar 2007 22:05:56 -0600 4, 20 -- Received: from [192.168.1.101] (68-112-237-5.dhcp.oxfr.ma.charter.com [68.112.237.5]) 4, 20 -- (authenticated bits=0) 4, 20 -- by race6.oit.umass.edu (8.13.7/8.13.7) with ESMTP id l2545oeh014738 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 4, 20 -- for {microscopy-at-microscopy.com} ; Sun, 4 Mar 2007 23:05:50 -0500 4, 20 -- Message-ID: {45EB97C2.7030608-at-research.umass.edu} 4, 20 -- Date: Sun, 04 Mar 2007 23:08:34 -0500 4, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 4, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.2) Gecko/20070222 SeaMonkey/1.1.1 4, 20 -- MIME-Version: 1.0 4, 20 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 4, 20 -- Subject: Re: Fw: [Microscopy] Source of supplies for Balzers/Baltec (FF, evaporators) 4, 20 -- References: {005c01c75da9$5be292a0$4e195c18-at-muchie} 4, 20 -- In-Reply-To: {005c01c75da9$5be292a0$4e195c18-at-muchie} 4, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I would like to assess the level of bacterial adhesion to the soft contact lenses surface with the SEM. Unfortunately I don?t have an access to environmental mode. Could anyone suggest the reliable approach to dehydrate soft contact lens without compromising its surface? Thank you in advance,
Albina
-- MIKHAYLOVA,ALBINA, PhD
==============================Original Headers============================== 6, 22 -- From amich-at-ufl.edu Mon Mar 5 13:45:14 2007 6, 22 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l25JjDwX003507 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 5 Mar 2007 13:45:14 -0600 6, 22 -- Received: from osgjas04.cns.ufl.edu (osgjas04.cns.ufl.edu [128.227.74.134]) 6, 22 -- by smtp.ufl.edu (8.13.7/8.13.7/2.5.9) with ESMTP id l25Jj6MA3076154 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 5 Mar 2007 14:45:07 -0500 6, 22 -- Message-ID: {1142537439.23911173123906749.JavaMail.osg-at-osgjas04.cns.ufl.edu} 6, 22 -- Date: Mon, 5 Mar 2007 14:45:06 -0500 (EST) 6, 22 -- From: "MIKHAYLOVA,ALBINA" {amich-at-ufl.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- Subject: SEM of bacterial adhesion to soft contact lenses 6, 22 -- MIME-Version: 1.0 6, 22 -- Content-Type: text/plain; format=flowed; charset=us-ascii 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-Mailer: GatorMail WebMail (http://GatorMail.sf.net/) 6, 22 -- X-Originating-IP: 70.152.33.129 [70.152.33.129] 6, 22 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0rc3 (smtp.ufl.edu [128.227.74.56]); Mon, 05 Mar 2007 14:45:07 -0500 (EST) 6, 22 -- X-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 6, 22 -- X-UFL-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 6, 22 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 6, 22 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both twigg-at-estd.nrl.navy.mil as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: twigg-at-estd.nrl.navy.mil Name: Mark Twigg
Organization: NRL
Title-Subject: [Filtered] Electrically Insulating Lacqer or Paint
Question: I am looking for an electrically-insulating lacquer or paint that does not need to be baked or heated in order to adhere to a metal surface.
You haven't stated the full details of your application so my suggestion may be useless. Does this need to go into a vacuum? Temperature range? Voltage you are insulating? Resistivity required?
I would suggest looking into the compounds used for insulating HV connections to avoid breakdown; generically these are called DAG or Electro-DAG; electronics supply houses or Amateur Radio suppliers have it.
Or check these for higher tech possibilities: http://www.cotronics.com/vo/cotr/ http://www.masterbond.com/
I have no interest in these companies....
Dale Callaham
twigg-at-estd.nrl.navy.mil wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both twigg-at-estd.nrl.navy.mil as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: twigg-at-estd.nrl.navy.mil } Name: Mark Twigg } } Organization: NRL } } Title-Subject: [Filtered] Electrically Insulating Lacqer or Paint } } Question: I am looking for an electrically-insulating lacquer or paint that does not need to be baked or heated in order to adhere to a metal surface. } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 12 -- From zaluzec-at-microscopy.com Tue Mar 6 08:32:00 2007 } 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l26EVx7k007200 } 6, 12 -- for {microscopy-at-microscopy.com} ; Tue, 6 Mar 2007 08:32:00 -0600 } 6, 12 -- Mime-Version: 1.0 } 6, 12 -- X-Sender: (Unverified) } 6, 12 -- Message-Id: {p06110407c2132bd02de0-at-[206.69.208.22]} } 6, 12 -- Date: Tue, 6 Mar 2007 08:31:59 -0600 } 6, 12 -- To: microscopy-at-microscopy.com } 6, 12 -- From: twigg-at-estd.nrl.navy.mil (by way of MicroscopyListserver) } 6, 12 -- Subject: viaWWW: Electrically Insulating Lacqer or Paint } 6, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 21 -- From dac-at-research.umass.edu Tue Mar 6 08:59:45 2007 6, 21 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l26ExjTk019449 6, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Mar 2007 08:59:45 -0600 6, 21 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 6, 21 -- (authenticated bits=0) 6, 21 -- by race1.oit.umass.edu (8.13.7/8.13.7) with ESMTP id l26Exc0W010924 6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 6, 21 -- Tue, 6 Mar 2007 09:59:38 -0500 6, 21 -- Message-ID: {45ED824F.3050905-at-research.umass.edu} 6, 21 -- Date: Tue, 06 Mar 2007 10:01:35 -0500 6, 21 -- From: Dale Callaham {dac-at-research.umass.edu} 6, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.2pre) Gecko/20070111 SeaMonkey/1.1 6, 21 -- MIME-Version: 1.0 6, 21 -- To: twigg-at-estd.nrl.navy.mil, Microscopy Listserver {Microscopy-at-microscopy.com} 6, 21 -- Subject: Re: [Microscopy] viaWWW: Electrically Insulating Lacqer or Paint 6, 21 -- References: {200703061442.l26EgUQM017415-at-ns.microscopy.com} 6, 21 -- In-Reply-To: {200703061442.l26EgUQM017415-at-ns.microscopy.com} 6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Try http://www.glyptal.com/ for glyptal insulating lacquer.
You can probably buy it at the electrical supply store, but the web site has neat history and a list of products.
Note: I do not work for them or have any financial interest, but I do use their insulating lacquer.
Oh - and it is very low vapor pressure when dry. Would not use it on a microscope vacuum system, but it used to be known as "the young metallurgist's friend" because when you simply could not find the leak in your vacuum furnace, the trick was to paint all soldered joints with glyptal while under vacuum; no more leak.
Regards, Andrew
At 08:33 AM 3/6/2007, you wrote:
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==============================Original Headers============================== 11, 32 -- From werner-at-rosharon.oilfield.slb.com Tue Mar 6 09:21:24 2007 11, 32 -- Received: from mail.slb.com (nammta01.sugar-land.nam.slb.com [163.188.150.130]) 11, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l26FLOie031234 11, 32 -- for {microscopy-at-microscopy.com} ; Tue, 6 Mar 2007 09:21:24 -0600 11, 32 -- Received: from pmxchannel_int-daemon.nammta01.sugar-land.nam.slb.com by 11, 32 -- nammta01.sugar-land.nam.slb.com 11, 32 -- (iPlanet Messaging Server 5.2 Patch 2 (built Jul 14 2004)) 11, 32 -- id {0JEH00D0DMNOIU-at-nammta01.sugar-land.nam.slb.com} for 11, 32 -- microscopy-at-microscopy.com; Tue, 06 Mar 2007 15:21:24 +0000 (GMT) 11, 32 -- Received: from usxsl052.slb.atosorigin-asp.com 11, 32 -- (usxsl052.slb.atosorigin-asp.com [199.6.136.167]) 11, 32 -- by nammta01.sugar-land.nam.slb.com 11, 32 -- (iPlanet Messaging Server 5.2 Patch 2 (built Jul 14 2004)) 11, 32 -- with ESMTP id {0JEH00BX6MNBTR-at-nammta01.sugar-land.nam.slb.com} for 11, 32 -- microscopy-at-microscopy.com; Tue, 06 Mar 2007 15:21:12 +0000 (GMT) 11, 32 -- Received: from WERNER1-OFS.rosharon.oilfield.slb.com ([163.188.46.5]) 11, 32 -- by us085mbx01.slb.atosorigin-asp.com 11, 32 -- (Sun Java System Messaging Server 6.2-1 (built Feb 24 2005)) 11, 32 -- with ESMTPSA id {0JEH00HC7MN81II0-at-us085mbx01.slb.atosorigin-asp.com} for 11, 32 -- microscopy-at-microscopy.com; Tue, 06 Mar 2007 15:21:09 +0000 (GMT) 11, 32 -- Date: Tue, 06 Mar 2007 09:21:07 -0600 11, 32 -- From: Andrew Werner {werner-at-rosharon.oilfield.slb.com} 11, 32 -- Subject: Re: [Microscopy] viaWWW: Electrically Insulating Lacqer or Paint 11, 32 -- In-reply-to: {200703061433.l26EXIxb008446-at-ns.microscopy.com} 11, 32 -- To: twigg-at-estd.nrl.navy.mil 11, 32 -- Cc: microscopy-at-microscopy.com 11, 32 -- Message-id: {6.2.1.2.2.20070306090900.0318d1c0-at-us1061-pop3.mail.slb.com} 11, 32 -- MIME-version: 1.0 11, 32 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2 11, 32 -- Content-type: text/plain; charset=us-ascii; format=flowed 11, 32 -- Content-transfer-encoding: 7BIT 11, 32 -- References: {200703061433.l26EXIxb008446-at-ns.microscopy.com} ==============================End of - Headers==============================
Mark, Like the others, since we're lacking some details, I can't promise that this is your solution, but there are specialty spray acrylics available from the likes of Newark Electronics that insulate at about 1kV/mil. I've had good luck with Krylon clear acrylic spray paint. I don't think it's substantially different (although we have some paint folks on the listserver who can correct me if I'm wrong) and it's cheap and easy to find.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: twigg-at-estd.nrl.navy.mil [mailto:twigg-at-estd.nrl.navy.mil] Sent: Tuesday, March 06, 2007 9:37 AM To: kenconverse-at-qualityimages.biz
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Email: twigg-at-estd.nrl.navy.mil Name: Mark Twigg
Organization: NRL
Title-Subject: [Filtered] Electrically Insulating Lacqer or Paint
Question: I am looking for an electrically-insulating lacquer or paint that does not need to be baked or heated in order to adhere to a metal surface.
==============================Original Headers============================== 6, 12 -- From zaluzec-at-microscopy.com Tue Mar 6 08:32:00 2007 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l26EVx7k007200 6, 12 -- for {microscopy-at-microscopy.com} ; Tue, 6 Mar 2007 08:32:00 -0600 6, 12 -- Mime-Version: 1.0 6, 12 -- X-Sender: (Unverified) 6, 12 -- Message-Id: {p06110407c2132bd02de0-at-[206.69.208.22]} 6, 12 -- Date: Tue, 6 Mar 2007 08:31:59 -0600 6, 12 -- To: microscopy-at-microscopy.com 6, 12 -- From: twigg-at-estd.nrl.navy.mil (by way of MicroscopyListserver) 6, 12 -- Subject: viaWWW: Electrically Insulating Lacqer or Paint 6, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
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==============================Original Headers============================== 24, 30 -- From kenconverse-at-qualityimages.biz Tue Mar 6 10:23:18 2007 24, 30 -- Received: from dpmailmta02.doteasy.com (dpmailmta02.doteasy.com [65.61.209.90]) 24, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l26GNHVU011421 24, 30 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 6 Mar 2007 10:23:18 -0600 24, 30 -- Received: from qualityimages.biz (unverified [192.168.101.16]) 24, 30 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id 64712674-1814644 24, 30 -- for multiple; Tue, 06 Mar 2007 09:24:25 -0800 24, 30 -- Received: from Ken [76.179.237.214] by qualityimages.biz with ESMTP 24, 30 -- (SMTPD32-8.05) id A56A6C4C0120; Tue, 06 Mar 2007 08:23:06 -0800 24, 30 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 24, 30 -- To: {twigg-at-estd.nrl.navy.mil} , 24, 30 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 24, 30 -- Subject: RE: [Microscopy] viaWWW: Electrically Insulating Lacqer or Paint 24, 30 -- Date: Tue, 6 Mar 2007 11:22:46 -0500 24, 30 -- Message-ID: {00d301c7600b$af1d2ca0$6401a8c0-at-Ken} 24, 30 -- MIME-Version: 1.0 24, 30 -- Content-Type: text/plain; 24, 30 -- charset="us-ascii" 24, 30 -- X-Priority: 3 (Normal) 24, 30 -- X-MSMail-Priority: Normal 24, 30 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 24, 30 -- Importance: Normal 24, 30 -- Thread-Index: Acdf/PYu9Jvs/7ZUTdulwHU7yvnqHwADDdPg 24, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 24, 30 -- In-Reply-To: {200703061437.l26EbK8w011961-at-ns.microscopy.com} 24, 30 -- X-IMSTrailer: __IMail_7__ 24, 30 -- X-IP-stats: Incoming Last 0, First 159, in=3583038, out=0, spam=0 ip=192.168.101.16 24, 30 -- X-Originating-IP: 192.168.101.16 24, 30 -- Content-Transfer-Encoding: 8bit 24, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l26GNHVU011421 ==============================End of - Headers==============================
Dear Mark, The classic for this purpose is stopping lacquer, available from suppliers of electro-polishing and polishing equipment. We get ours from Tolber. It is a red lacquer, thinned and/or removed with acetone, that is painted on to mask off parts that are not to be electro-polished. Good luck,
-----Original Message----- X-from: twigg-at-estd.nrl.navy.mil [mailto:twigg-at-estd.nrl.navy.mil] Sent: March 6, 2007 6:42 AM To: mager-at-interchange.ubc.ca
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Email: twigg-at-estd.nrl.navy.mil Name: Mark Twigg
Organization: NRL
Title-Subject: [Filtered] Electrically Insulating Lacqer or Paint
Question: I am looking for an electrically-insulating lacquer or paint that does not need to be baked or heated in order to adhere to a metal surface.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both twigg-at-estd.nrl.navy.mil as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: twigg-at-estd.nrl.navy.mil Name: Mark Twigg
Organization: NRL
Title-Subject: [Filtered] Electrically Insulating Lacquer or Paint
Question: I agree with the inquiries that I should have provided a more detailed description of my needs. The lacquer or paint that I need should have the following attributes:
1. Electrically isulating, but it only needs to stand up to 20 V.
2. Low vapor pressure for high vacuum use--it will coat a small region (~3 mm x 3mm) of a transmission electron microscope sample holder.
3. Must go on without heat or baking--I don't want to bake my sample holder.
4. It will not need to withstand high temperatures. It will be used at room temperature.
Mark, I'd be inclined to go with the clear acrylic spray paint. You can keep the layer extremely thin to avoid any serious outgassing. I'm assuming that the beam is not going to hit this area, since you said the temperature would be room temperature. The other items mentioned would probably also work well, although you might have to deal with a considerably thicker layer and, therefore, more significant outgassing initially.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: twigg-at-estd.nrl.navy.mil [mailto:twigg-at-estd.nrl.navy.mil] Sent: Tuesday, March 06, 2007 1:57 PM To: kenconverse-at-qualityimages.biz
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying
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Email: twigg-at-estd.nrl.navy.mil Name: Mark Twigg
Organization: NRL
Title-Subject: [Filtered] Electrically Insulating Lacquer or Paint
Question: I agree with the inquiries that I should have provided a more detailed description of my needs. The lacquer or paint that I need should have the following attributes:
1. Electrically isulating, but it only needs to stand up to 20 V.
2. Low vapor pressure for high vacuum use--it will coat a small region (~3 mm x 3mm) of a transmission electron microscope sample holder.
3. Must go on without heat or baking--I don't want to bake my sample holder.
4. It will not need to withstand high temperatures. It will be used at room temperature.
==============================Original Headers============================== 12, 12 -- From zaluzec-at-microscopy.com Tue Mar 6 12:54:21 2007 12, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 12, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l26IsLrh004927 12, 12 -- for {microscopy-at-microscopy.com} ; Tue, 6 Mar 2007 12:54:21 -0600 12, 12 -- Mime-Version: 1.0 12, 12 -- X-Sender: (Unverified) 12, 12 -- Message-Id: {p06110400c2136945452a-at-[206.69.208.22]} 12, 12 -- Date: Tue, 6 Mar 2007 12:54:21 -0600 12, 12 -- To: microscopy-at-microscopy.com 12, 12 -- From: twigg-at-estd.nrl.navy.mil (by way of MicroscopyListserver) 12, 12 -- Subject: viaWWW: More 0n - Electrically Insulating Lacquer or Paint 12, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
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==============================Original Headers============================== 30, 30 -- From kenconverse-at-qualityimages.biz Tue Mar 6 14:17:29 2007 30, 30 -- Received: from dpmailmta02.doteasy.com (dpmailmta02.doteasy.com [65.61.209.90]) 30, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l26KHT8i017551 30, 30 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 6 Mar 2007 14:17:29 -0600 30, 30 -- Received: from qualityimages.biz (unverified [192.168.101.16]) 30, 30 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id 64759480-1814644 30, 30 -- for multiple; Tue, 06 Mar 2007 13:18:40 -0800 30, 30 -- Received: from Ken [76.179.237.214] by qualityimages.biz with ESMTP 30, 30 -- (SMTPD32-8.05) id AC53709F0080; Tue, 06 Mar 2007 12:17:23 -0800 30, 30 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 30, 30 -- To: {twigg-at-estd.nrl.navy.mil} , 30, 30 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 30, 30 -- Subject: RE: [Microscopy] viaWWW: More 0n - Electrically Insulating Lacquer or Paint 30, 30 -- Date: Tue, 6 Mar 2007 15:16:58 -0500 30, 30 -- Message-ID: {011701c7602c$6a0e5460$6401a8c0-at-Ken} 30, 30 -- MIME-Version: 1.0 30, 30 -- Content-Type: text/plain; 30, 30 -- charset="us-ascii" 30, 30 -- X-Priority: 3 (Normal) 30, 30 -- X-MSMail-Priority: Normal 30, 30 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 30, 30 -- Importance: Normal 30, 30 -- Thread-Index: AcdgITYHr00ilmc1QtOqW7egb+T0sQACoRKQ 30, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 30, 30 -- In-Reply-To: {200703061856.l26Iuo7j009617-at-ns.microscopy.com} 30, 30 -- X-IMSTrailer: __IMail_7__ 30, 30 -- X-IP-stats: Incoming Last 0, First 159, in=3585585, out=0, spam=0 ip=192.168.101.16 30, 30 -- X-Originating-IP: 192.168.101.16 30, 30 -- Content-Transfer-Encoding: 8bit 30, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l26KHT8i017551 ==============================End of - Headers==============================
Since it is a low voltage insulation, what about just a dab of formvar in chloroform or 1,2-dichlorethane? Make it a bit thick (2% - 5% ?) so it doesn't flow too much. Formvar is the insulation once (still?) used on "magnet wire" - they probably use more advanced things for hi-temp purposes these days. But it should give reasonable adhesion to metal (as in the wire usage), should dry/outgas at RT and be OK in a high vacuum (as in thin support films). And most EM labs have some. In a pinch, polystyrene dissolved in CHCl3 will do the same - people use it for films (here you could use a bit of dispo petri dish...).
Dale
kenconverse-at-qualityimages.biz wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Mark, } I'd be inclined to go with the clear acrylic spray paint. You can keep the } layer extremely thin to avoid any serious outgassing. I'm assuming that the } beam is not going to hit this area, since you said the temperature would be } room temperature. The other items mentioned would probably also work well, } although you might have to deal with a considerably thicker layer and, } therefore, more significant outgassing initially. } } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } } -----Original Message----- } X-from: twigg-at-estd.nrl.navy.mil [mailto:twigg-at-estd.nrl.navy.mil] } Sent: Tuesday, March 06, 2007 1:57 PM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] viaWWW: More 0n - Electrically Insulating Lacquer or } Paint } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver using the } WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } } please copy both twigg-at-estd.nrl.navy.mil as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: twigg-at-estd.nrl.navy.mil } Name: Mark Twigg } } Organization: NRL } } Title-Subject: [Filtered] Electrically Insulating Lacquer or Paint } } Question: I agree with the inquiries that I should have provided a more } detailed description of my needs. The lacquer or paint that I need should } have the following attributes: } } 1. Electrically isulating, but it only needs to stand up to 20 V. } } 2. Low vapor pressure for high vacuum use--it will coat a small region (~3 } mm x 3mm) of a transmission electron microscope sample holder. } } 3. Must go on without heat or baking--I don't want to bake my sample } holder. } } 4. It will not need to withstand high temperatures. It will be used at } room temperature. } } Thanks again, } } Mark } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 12, 12 -- From zaluzec-at-microscopy.com Tue Mar 6 12:54:21 2007 12, 12 -- } Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 12, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l26IsLrh004927 } 12, 12 -- for {microscopy-at-microscopy.com} ; Tue, 6 Mar 2007 12:54:21 } -0600 } 12, 12 -- Mime-Version: 1.0 } 12, 12 -- X-Sender: (Unverified) } 12, 12 -- Message-Id: {p06110400c2136945452a-at-[206.69.208.22]} } 12, 12 -- Date: Tue, 6 Mar 2007 12:54:21 -0600 } 12, 12 -- To: microscopy-at-microscopy.com } 12, 12 -- From: twigg-at-estd.nrl.navy.mil (by way of MicroscopyListserver) 12, } 12 -- Subject: viaWWW: More 0n - Electrically Insulating Lacquer or Paint } 12, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== } } } } } } _________________________________________________________________ } Need personalized email and website? Look no further. It's easy } with Doteasy $0 Web Hosting! Learn more at www.doteasy.com } } } ==============================Original Headers============================== } 30, 30 -- From kenconverse-at-qualityimages.biz Tue Mar 6 14:17:29 2007 } 30, 30 -- Received: from dpmailmta02.doteasy.com (dpmailmta02.doteasy.com [65.61.209.90]) } 30, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l26KHT8i017551 } 30, 30 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 6 Mar 2007 14:17:29 -0600 } 30, 30 -- Received: from qualityimages.biz (unverified [192.168.101.16]) } 30, 30 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id 64759480-1814644 } 30, 30 -- for multiple; Tue, 06 Mar 2007 13:18:40 -0800 } 30, 30 -- Received: from Ken [76.179.237.214] by qualityimages.biz with ESMTP } 30, 30 -- (SMTPD32-8.05) id AC53709F0080; Tue, 06 Mar 2007 12:17:23 -0800 } 30, 30 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } 30, 30 -- To: {twigg-at-estd.nrl.navy.mil} , } 30, 30 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} } 30, 30 -- Subject: RE: [Microscopy] viaWWW: More 0n - Electrically Insulating Lacquer or Paint } 30, 30 -- Date: Tue, 6 Mar 2007 15:16:58 -0500 } 30, 30 -- Message-ID: {011701c7602c$6a0e5460$6401a8c0-at-Ken} } 30, 30 -- MIME-Version: 1.0 } 30, 30 -- Content-Type: text/plain; } 30, 30 -- charset="us-ascii" } 30, 30 -- X-Priority: 3 (Normal) } 30, 30 -- X-MSMail-Priority: Normal } 30, 30 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 } 30, 30 -- Importance: Normal } 30, 30 -- Thread-Index: AcdgITYHr00ilmc1QtOqW7egb+T0sQACoRKQ } 30, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 } 30, 30 -- In-Reply-To: {200703061856.l26Iuo7j009617-at-ns.microscopy.com} } 30, 30 -- X-IMSTrailer: __IMail_7__ } 30, 30 -- X-IP-stats: Incoming Last 0, First 159, in=3585585, out=0, spam=0 ip=192.168.101.16 } 30, 30 -- X-Originating-IP: 192.168.101.16 } 30, 30 -- Content-Transfer-Encoding: 8bit } 30, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l26KHT8i017551 } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 22 -- From dac-at-research.umass.edu Tue Mar 6 14:48:14 2007 5, 22 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l26KmD0a029578 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Mar 2007 14:48:13 -0600 5, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 5, 22 -- (authenticated bits=0) 5, 22 -- by race4.oit.umass.edu (8.13.7/8.13.7) with ESMTP id l26KmDMc031233 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Mar 2007 15:48:13 -0500 5, 22 -- Message-ID: {45EDD402.4060909-at-research.umass.edu} 5, 22 -- Date: Tue, 06 Mar 2007 15:50:10 -0500 5, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 5, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.2pre) Gecko/20070111 SeaMonkey/1.1 5, 22 -- MIME-Version: 1.0 5, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 5, 22 -- Subject: Re: [Microscopy] RE: viaWWW: More 0n - Electrically Insulating Lacquer 5, 22 -- or Paint 5, 22 -- References: {200703062023.l26KNQNU027537-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {200703062023.l26KNQNU027537-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
McMaster Carr www.mcmaster.com has 3 in spray cans 7437K17 7437K16 7437K18 and if it doesn't need to flex the age old solution of Shellac should work as well.
Gordon Gordon Couger Stillwater OK www.science-info.net
twigg-at-estd.nrl.navy.mil wrote: } Email: twigg-at-estd.nrl.navy.mil } Name: Mark Twigg } } Organization: NRL } } Title-Subject: [Filtered] Electrically Insulating Lacqer or Paint } } Question: I am looking for an electrically-insulating lacquer or paint that does not need to be baked or heated in order to adhere to a metal surface. } } -
==============================Original Headers============================== 4, 19 -- From gcouger-at-science-info.net Tue Mar 6 15:11:03 2007 4, 19 -- Received: from smtp106.biz.mail.mud.yahoo.com (smtp106.biz.mail.mud.yahoo.com [68.142.200.254]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l26LB3rQ008723 4, 19 -- for {microscopy-at-microscopy.com} ; Tue, 6 Mar 2007 15:11:03 -0600 4, 19 -- Received: (qmail 90518 invoked from network); 6 Mar 2007 21:11:02 -0000 4, 19 -- Received: from unknown (HELO ?192.168.1.115?) (gcouger-at-science-info.net-at-74.195.225.38 with plain) 4, 19 -- by smtp106.biz.mail.mud.yahoo.com with SMTP; 6 Mar 2007 21:11:02 -0000 4, 19 -- X-YMail-OSG: oaKlNNQVM1kckHRUX3iOWLKeA1pAErDRiVICtdaDttmpiDDlTdXlN99JzqiUeGYT08zC393xjX_ApgKyTq7RzPRIiAZUSQTjSYaRoeKlCSUVgBZbtbVZU33jvUPuOWeyXQJhpLV7k82p_xc- 4, 19 -- Message-ID: {45EDD8E7.9040605-at-science-info.net} 4, 19 -- Date: Tue, 06 Mar 2007 15:11:03 -0600 4, 19 -- From: Gordon Couger {gcouger-at-science-info.net} 4, 19 -- User-Agent: Thunderbird 1.5.0.10 (Macintosh/20070221) 4, 19 -- MIME-Version: 1.0 4, 19 -- To: twigg-at-estd.nrl.navy.mil, microscopy-at-microscopy.com 4, 19 -- Subject: Re: [Microscopy] viaWWW: Electrically Insulating Lacqer or Paint 4, 19 -- References: {200703061443.l26Ehk3A018660-at-ns.microscopy.com} 4, 19 -- In-Reply-To: {200703061443.l26Ehk3A018660-at-ns.microscopy.com} 4, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Can anyone recommend an inexpensive adaptor that will allow me to couple my Fuji FinePix E550 digital camera to the trinocular port of my Olympus BX40?
Thanks.
Gary Gill
==============================Original Headers============================== 2, 17 -- From garygill-at-dcla.com Wed Mar 7 09:22:30 2007 2, 17 -- Received: from sturgeon.dcla.com (dcla.com [207.67.84.53] (may be forged)) 2, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l27FMUu3007323 2, 17 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Mar 2007 09:22:30 -0600 2, 17 -- Received: by sturgeon.dcla.com with Internet Mail Service (5.5.2653.19) 2, 17 -- id {GD4DS61B} ; Wed, 7 Mar 2007 10:22:24 -0500 2, 17 -- Message-ID: {0420BA36C221714593AA90E7F92CAF4706E9E1BC-at-sturgeon.dcla.com} 2, 17 -- From: Gary Gill {garygill-at-dcla.com} 2, 17 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-Microscopy.Com} 2, 17 -- Subject: LM: camera adaptor for photomicrography 2, 17 -- Date: Wed, 7 Mar 2007 10:22:15 -0500 2, 17 -- MIME-Version: 1.0 2, 17 -- X-Mailer: Internet Mail Service (5.5.2653.19) 2, 17 -- Content-Type: text/plain; 2, 17 -- charset="iso-8859-1" 2, 17 -- Content-Transfer-Encoding: 8bit 2, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l27FMUu3007323 ==============================End of - Headers==============================
I have not used them, nor have any interest, but you might check with: www.scopetronix.com
Woody White BWXT Services
-----Original Message----- X-from: garygill-at-dcla.com [mailto:garygill-at-dcla.com] Sent: Wednesday, March 07, 2007 10:23 AM To: White, Woody N.
Can anyone recommend an inexpensive adaptor that will allow me to couple my Fuji FinePix E550 digital camera to the trinocular port of my Olympus BX40?
Thanks.
Gary Gill
==============================Original Headers============================== 2, 17 -- From garygill-at-dcla.com Wed Mar 7 09:22:30 2007 2, 17 -- Received: from sturgeon.dcla.com (dcla.com [207.67.84.53] (may be forged)) 2, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l27FMUu3007323 2, 17 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Mar 2007 09:22:30 -0600 2, 17 -- Received: by sturgeon.dcla.com with Internet Mail Service (5.5.2653.19) 2, 17 -- id {GD4DS61B} ; Wed, 7 Mar 2007 10:22:24 -0500 2, 17 -- Message-ID: {0420BA36C221714593AA90E7F92CAF4706E9E1BC-at-sturgeon.dcla.com} 2, 17 -- From: Gary Gill {garygill-at-dcla.com} 2, 17 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-Microscopy.Com} 2, 17 -- Subject: LM: camera adaptor for photomicrography 2, 17 -- Date: Wed, 7 Mar 2007 10:22:15 -0500 2, 17 -- MIME-Version: 1.0 2, 17 -- X-Mailer: Internet Mail Service (5.5.2653.19) 2, 17 -- Content-Type: text/plain; 2, 17 -- charset="iso-8859-1" 2, 17 -- Content-Transfer-Encoding: 8bit 2, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l27FMUu3007323 ==============================End of - Headers==============================
==============================Original Headers============================== 11, 28 -- From nwwhite-at-bwxt.com Wed Mar 7 09:58:38 2007 11, 28 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l27FwboS019356 11, 28 -- for {microscopy-at-msa.microscopy.com} ; Wed, 7 Mar 2007 09:58:37 -0600 11, 28 -- Received: from ([131.184.13.224]) 11, 28 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.4224394; 11, 28 -- Wed, 07 Mar 2007 10:58:22 -0500 11, 28 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); 11, 28 -- Wed, 7 Mar 2007 10:58:22 -0500 11, 28 -- Content-class: urn:content-classes:message 11, 28 -- MIME-Version: 1.0 11, 28 -- Content-Type: text/plain; 11, 28 -- charset="iso-8859-1" 11, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 28 -- Subject: RE: [Microscopy] LM: camera adaptor for photomicrography 11, 28 -- Date: Wed, 7 Mar 2007 10:58:22 -0500 11, 28 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB703D15E-at-BWXSPO01.BWXS.BWXTECH.NET} 11, 28 -- In-Reply-To: {200703071523.l27FNT8B008153-at-ns.microscopy.com} 11, 28 -- X-MS-Has-Attach: 11, 28 -- X-MS-TNEF-Correlator: 11, 28 -- Thread-Topic: [Microscopy] LM: camera adaptor for photomicrography 11, 28 -- Thread-Index: AcdgzMBxTOLx2DbPQXu83oGj/oL8NAABFkCg 11, 28 -- From: "White, Woody N." {NWWhite-at-bwxt.com} 11, 28 -- To: {garygill-at-dcla.com} , 11, 28 -- "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 11, 28 -- X-OriginalArrivalTime: 07 Mar 2007 15:58:22.0923 (UTC) FILETIME=[6EF379B0:01C760D1] 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l27FwboS019356 ==============================End of - Headers==============================
Scopetronix apparently doesn't exist anymore. Their address is an empty building, no one answers their phone. If anyone turns them up, please let me know.
Ron Anderson, Editor Microscopy Today
NWWhite-at-bwxt.com wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I have not used them, nor have any interest, but you might check with: } www.scopetronix.com } } Woody White } BWXT Services } } } -----Original Message----- } X-from: garygill-at-dcla.com [mailto:garygill-at-dcla.com] } Sent: Wednesday, March 07, 2007 10:23 AM } To: White, Woody N. } Subject: [Microscopy] LM: camera adaptor for photomicrography } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Can anyone recommend an inexpensive adaptor that will allow me to couple my } Fuji FinePix E550 digital camera to the trinocular port of my Olympus BX40? } } Thanks. } } Gary Gill } } } } ==============================Original Headers============================== } 2, 17 -- From garygill-at-dcla.com Wed Mar 7 09:22:30 2007 } 2, 17 -- Received: from sturgeon.dcla.com (dcla.com [207.67.84.53] (may be forged)) } 2, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l27FMUu3007323 } 2, 17 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Mar 2007 09:22:30 -0600 } 2, 17 -- Received: by sturgeon.dcla.com with Internet Mail Service (5.5.2653.19) } 2, 17 -- id {GD4DS61B} ; Wed, 7 Mar 2007 10:22:24 -0500 } 2, 17 -- Message-ID: {0420BA36C221714593AA90E7F92CAF4706E9E1BC-at-sturgeon.dcla.com} } 2, 17 -- From: Gary Gill {garygill-at-dcla.com} } 2, 17 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-Microscopy.Com} } 2, 17 -- Subject: LM: camera adaptor for photomicrography } 2, 17 -- Date: Wed, 7 Mar 2007 10:22:15 -0500 } 2, 17 -- MIME-Version: 1.0 } 2, 17 -- X-Mailer: Internet Mail Service (5.5.2653.19) } 2, 17 -- Content-Type: text/plain; } 2, 17 -- charset="iso-8859-1" } 2, 17 -- Content-Transfer-Encoding: 8bit } 2, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l27FMUu3007323 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 11, 28 -- From nwwhite-at-bwxt.com Wed Mar 7 09:58:38 2007 } 11, 28 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) } 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l27FwboS019356 } 11, 28 -- for {microscopy-at-msa.microscopy.com} ; Wed, 7 Mar 2007 09:58:37 -0600 } 11, 28 -- Received: from ([131.184.13.224]) } 11, 28 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.4224394; } 11, 28 -- Wed, 07 Mar 2007 10:58:22 -0500 } 11, 28 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); } 11, 28 -- Wed, 7 Mar 2007 10:58:22 -0500 } 11, 28 -- Content-class: urn:content-classes:message } 11, 28 -- MIME-Version: 1.0 } 11, 28 -- Content-Type: text/plain; } 11, 28 -- charset="iso-8859-1" } 11, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 11, 28 -- Subject: RE: [Microscopy] LM: camera adaptor for photomicrography } 11, 28 -- Date: Wed, 7 Mar 2007 10:58:22 -0500 } 11, 28 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB703D15E-at-BWXSPO01.BWXS.BWXTECH.NET} } 11, 28 -- In-Reply-To: {200703071523.l27FNT8B008153-at-ns.microscopy.com} } 11, 28 -- X-MS-Has-Attach: } 11, 28 -- X-MS-TNEF-Correlator: } 11, 28 -- Thread-Topic: [Microscopy] LM: camera adaptor for photomicrography } 11, 28 -- Thread-Index: AcdgzMBxTOLx2DbPQXu83oGj/oL8NAABFkCg } 11, 28 -- From: "White, Woody N." {NWWhite-at-bwxt.com} } 11, 28 -- To: {garygill-at-dcla.com} , } 11, 28 -- "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} } 11, 28 -- X-OriginalArrivalTime: 07 Mar 2007 15:58:22.0923 (UTC) FILETIME=[6EF379B0:01C760D1] } 11, 28 -- Content-Transfer-Encoding: 8bit } 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l27FwboS019356 } ==============================End of - Headers============================== } } }
==============================Original Headers============================== 4, 20 -- From microscopytoday-at-tampabay.rr.com Wed Mar 7 10:26:28 2007 4, 20 -- Received: from ms-smtp-07.tampabay.rr.com (ms-smtp-07.tampabay.rr.com [65.32.5.139]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l27GQSwl031145 4, 20 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Mar 2007 10:26:28 -0600 4, 20 -- Received: from [127.0.0.1] (rrcs-24-73-73-214.se.biz.rr.com [24.73.73.214]) 4, 20 -- by ms-smtp-07.tampabay.rr.com (8.13.6/8.13.6) with ESMTP id l27GQN4V022319; 4, 20 -- Wed, 7 Mar 2007 11:26:25 -0500 (EST) 4, 20 -- Message-ID: {45EEE7AE.3070304-at-tampabay.rr.com} 4, 20 -- Date: Wed, 07 Mar 2007 11:26:22 -0500 4, 20 -- From: Microscopy Today {microscopytoday-at-tampabay.rr.com} 4, 20 -- User-Agent: Thunderbird 1.5.0.10 (Windows/20070221) 4, 20 -- MIME-Version: 1.0 4, 20 -- To: NWWhite-at-bwxt.com, garygill-at-dcla.com, 4, 20 -- Listserver {Microscopy-at-Microscopy.Com} 4, 20 -- Subject: Re: [Microscopy] RE: LM: camera adaptor for photomicrography 4, 20 -- References: {200703071558.l27FwtcP019558-at-ns.microscopy.com} 4, 20 -- In-Reply-To: {200703071558.l27FwtcP019558-at-ns.microscopy.com} 4, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 20 -- Content-Transfer-Encoding: 7bit 4, 20 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
Look into both Scopetronix and the Microscope Store CamAdapter kits.
Latter is at www.microscope-store.com/index.php/cPath/12_15
We have both kits available, but haven't had time to evaluate carefully side-by-side. I think the latter may include some features and a range of specific add-on adapters that cover more ground; add-on kit for your Fuji is listed at www.microscope-store.com/index.php/cPath/12_19
I recommend you talk to both companies by phone and take notes. -mike reedy-
At 9:27 AM -0600 3/7/07, garygill-at-dcla.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
Try Bobby Martin at Martin Microscope (www.martinmicroscope.com). I don't know about the FinePix, but they make and sell an adapter for the Canon digital cameras. Disclaimer: No commercial connection except that of a satisfied customer. Julian
} } Can anyone recommend an inexpensive adaptor that will allow me to couple my } } Fuji FinePix E550 digital camera to the trinocular port of my Olympus BX40? } } } } Thanks. } } } } Gary Gill } }
-- Julian P.S. Smith III Dept. of Biology Winthrop University 520 Cherry Rd. Rock Hill, SC 29733
Someone at The Microscope Store is emailing me information. Total cost is about $300. Thanks for the leads, Mike and everyone.
Gary Gill
-----Original Message----- X-from: Mike Reedy [mailto:mike.reedy-at-cellbio.duke.edu] Sent: Wednesday, March 07, 2007 12:22 PM To: garygill-at-dcla.com Cc: Microscopy Listserver
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
O.k., I'm looking for some help in understanding electro-magnetic fields, and the sources generating them.
Here's the issue in my lab we've gone from 0.46mG to 8.7mG in X-axis EMF-AC, and 1.1mG to 15.2mG in Z-axis EMF-AC. These obviously push beyond specs for the SEM in the room.
So I am looking for an education. I have no idea of the context to put this in. What would or could cause these kinds of EM Field increases (15 to 20 times)? A single new 110V outlet? A new 1000W UPS in a near by room? A 480V feeder line? A new HVAC blower motor?
These were measurements were made in the middle of a 10ft x 10ft x 9ft room (3.2m x 3.2m x 3m) Next to an SEM, in the Off state (no power). I operate the rooms on two sides and below, and I have checked the labs on the other lateral sides and the floor above - no new equipment or significant electrical services within at least 15 to 50 feet in these spaces. BUT "signifcant" what is significant? Am I looking for a new 110v outlet? or a computer UPS? Or am I looking for something in the next building? Or 150feet down the hall? I have not dug through the ceilings yet to look for any "hidden" changes but again I do not know what I should be looking for.
Am I looking for an induced current coming through an eithernet cable?
I do not have a hand held gauss-meter to track things down. Do I need one?
Any help would be great. Thanks folks.
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"A main-frame: The biggest PC peripheral available."
==============================Original Headers============================== 11, 29 -- From edelmare-at-muohio.edu Wed Mar 7 12:11:44 2007 11, 29 -- Received: from spamfirewall.muohio.edu (wallaby.mcs.muohio.edu [134.53.6.28]) 11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l27IBiOK024783 11, 29 -- for {microscopy-at-Microscopy.com} ; Wed, 7 Mar 2007 12:11:44 -0600 11, 29 -- X-ASG-Debug-ID: 1173291103-52a900520000-Dem1zR 11, 29 -- X-Barracuda-URL: http://134.53.6.28:80/cgi-bin/mark.cgi 11, 29 -- X-Barracuda-Connect: mulnx23.mcs.muohio.edu[134.53.6.10] 11, 29 -- X-Barracuda-Start-Time: 1173291103 11, 29 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 11, 29 -- by spamfirewall.muohio.edu (Spam Firewall) with ESMTP id 94100494B7 11, 29 -- for {microscopy-at-Microscopy.com} ; Wed, 7 Mar 2007 13:11:43 -0500 (EST) 11, 29 -- Received: from [192.168.1.23] ([134.53.14.105]) 11, 29 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l27IBhLq020342 11, 29 -- for {microscopy-at-Microscopy.com} ; Wed, 7 Mar 2007 13:11:43 -0500 11, 29 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 11, 29 -- To: microscopy-at-Microscopy.com 11, 29 -- Date: Wed, 07 Mar 2007 13:11:43 -0500 11, 29 -- MIME-Version: 1.0 11, 29 -- X-ASG-Orig-Subj: EM Field sources 11, 29 -- Subject: EM Field sources 11, 29 -- Message-ID: {45EEBA0F.27061.1EA342A-at-edelmare.muohio.edu} 11, 29 -- Priority: normal 11, 29 -- X-mailer: Pegasus Mail for Windows (4.41) 11, 29 -- Content-type: text/plain; charset=US-ASCII 11, 29 -- Content-transfer-encoding: 7BIT 11, 29 -- Content-description: Mail message body 11, 29 -- X-Barracuda-Virus-Scanned: by Barracuda Spam Firewall at muohio.edu 11, 29 -- X-Barracuda-Spam-Score: 0.00 11, 29 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=3.5 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=1000.0 ==============================End of - Headers==============================
On Mar 7, 2007, at 9:37 AM, PWebster-at-hei.org wrote:
} We need a way to catalog stored images (EM & LM) together with } experimental } data (text) for access by in-house researchers as well as off-site } users. } } Does anyone know of any commercial products available that would fit } our } needs? } } We have someone here who would like to try to develop this but it will } be a } waste of time and money if something is already available. } Dear Paul, Are you talking about film or digital images? If the latter, Leginon not only collects data automatically from a selection of targets, but it also archives all the data in a database--I think that feature alone is worth using Leginon for. The program is free, although someone from your lab will have to attend a training class (also free). Leginon requires pretty large computer resources, but if you are archiving a lot of images you will need that in any case. I am not connected with the purveyors (Clint Potter & Bridget Carrigher) of Leginon except as a satisfied user, but, in the interest of full disclosure, Christian Suloway, the developer of many of the modules in Leginon, is a graduate student here, so we do have better access to fixing bugs than the average installation. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Wed Mar 7 12:38:41 2007 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l27Icew6004076 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 7 Mar 2007 12:38:40 -0600 4, 22 -- Received: from earth-dog.caltech.edu (earth-dog [192.168.1.3]) 4, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 861182F500 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 7 Mar 2007 10:38:39 -0800 (PST) 4, 22 -- Received: from [192.168.159.233] (jpix-01.caltech.edu [131.215.2.133]) 4, 22 -- by wood-ox.its.caltech.edu (Postfix) with ESMTP id 482582F3CF 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 7 Mar 2007 10:38:38 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v624) 4, 22 -- In-Reply-To: {200703071737.l27HbFNP010033-at-ns.microscopy.com} 4, 22 -- References: {200703071737.l27HbFNP010033-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {f2166ae2d56bcdafdfe6f4034a14e9e0-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] EM & LM Digital database 4, 22 -- Date: Wed, 7 Mar 2007 10:47:57 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.624) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.3.3 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both martini-at-accurel.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: martini-at-accurel.com Name: Martin Izquierdo
Organization: Accurel
Title-Subject: [Filtered] Gatan duomill spares
Question: After rummaging through some cabinets I found some Gatan Duomill spares. I have some o-rings, cathodes, even a autoterminator (and other misc duomill related things).
All the items are "free", but you do have to pay for the shipping.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (greta.rennings-at-web.de) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, March 7, 2007 at 13:41:04 ---------------------------------------------------------------------------
Email: greta.rennings-at-web.de Name: Greta Rennings
Organization: KU Leuven
Education: Graduate College
Location: Leuven, Brabant, Belgium
Question: Dear ladies and gentlemen,
do you have an idea which microscopic technique would be suitable for analysis of paper? Firstly for 3D I did trials with CLSM, which were not truly successful as grey scale differences were too low. Other techniques I know would require splitting or sectioning, as far as I know- do you have further experience? Secondly for 2D I tried light microscopy and SEM, but grey scale differences were to low here too in order to differentiate between components. Could TEM be useful? What could help to get better distinguishable features?
Thank you very much in advance for your support! Kind regards, Greta
To those of you who have gone digital and may have an enlarger collecting dust I might like to hear from you. I have rekindled an interest in B&W photography and am in need of a used enlarger. Because shipping will be a major expense I would initially like to limit my search to the southern Ontario/ upper New York state areas. If you have an enlarger that you have been considering for disposal please contact me.
Thanks,
Paul
Paul J. Gerroir
Microscopy Materials Characterization Xerox Research Centre of Canada 2660 Speakman Drive Mississauga, Ontario L5K 2L1
-----Original Message----- X-from: Jones,Dr.,Paul-James AN BIP-US-R Sent: Thursday, March 08, 2007 7:26 AM To: Coleman,Dr.,James AN BIP-US-R
O.k., I'm looking for some help in understanding electro-magnetic fields, and the sources generating them.
Here's the issue in my lab we've gone from 0.46mG to 8.7mG in X-axis EMF-AC, and 1.1mG to 15.2mG in Z-axis EMF-AC. These obviously push beyond specs for the SEM in the room.
So I am looking for an education. I have no idea of the context to put this in. What would or could cause these kinds of EM Field increases (15 to 20 times)? A single new 110V outlet? A new 1000W UPS in a near by room? A 480V feeder line? A new HVAC blower motor?
These were measurements were made in the middle of a 10ft x 10ft x 9ft room (3.2m x 3.2m x 3m) Next to an SEM, in the Off state (no power). I operate the rooms on two sides and below, and I have checked the labs on the other lateral sides and the floor above - no new equipment or significant electrical services within at least 15 to 50 feet in these spaces. BUT "signifcant" what is significant? Am I looking for a new 110v outlet? or a computer UPS? Or am I looking for something in the next building? Or 150feet down the hall? I have not dug through the ceilings yet to look for any "hidden" changes but again I do not know what I should be looking for.
Am I looking for an induced current coming through an eithernet cable?
I do not have a hand held gauss-meter to track things down. Do I need one?
Any help would be great. Thanks folks.
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"A main-frame: The biggest PC peripheral available."
==============================Original Headers============================== 11, 29 -- From edelmare-at-muohio.edu Wed Mar 7 12:11:44 2007 11, 29 -- Received: from spamfirewall.muohio.edu (wallaby.mcs.muohio.edu [134.53.6.28]) 11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l27IBiOK024783 11, 29 -- for {microscopy-at-Microscopy.com} ; Wed, 7 Mar 2007 12:11:44 -0600 11, 29 -- X-ASG-Debug-ID: 1173291103-52a900520000-Dem1zR 11, 29 -- X-Barracuda-URL: http://134.53.6.28:80/cgi-bin/mark.cgi 11, 29 -- X-Barracuda-Connect: mulnx23.mcs.muohio.edu[134.53.6.10] 11, 29 -- X-Barracuda-Start-Time: 1173291103 11, 29 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 11, 29 -- by spamfirewall.muohio.edu (Spam Firewall) with ESMTP id 94100494B7 11, 29 -- for {microscopy-at-Microscopy.com} ; Wed, 7 Mar 2007 13:11:43 -0500 (EST) 11, 29 -- Received: from [192.168.1.23] ([134.53.14.105]) 11, 29 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l27IBhLq020342 11, 29 -- for {microscopy-at-Microscopy.com} ; Wed, 7 Mar 2007 13:11:43 -0500 11, 29 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 11, 29 -- To: microscopy-at-Microscopy.com 11, 29 -- Date: Wed, 07 Mar 2007 13:11:43 -0500 11, 29 -- MIME-Version: 1.0 11, 29 -- X-ASG-Orig-Subj: EM Field sources 11, 29 -- Subject: EM Field sources 11, 29 -- Message-ID: {45EEBA0F.27061.1EA342A-at-edelmare.muohio.edu} 11, 29 -- Priority: normal 11, 29 -- X-mailer: Pegasus Mail for Windows (4.41) 11, 29 -- Content-type: text/plain; charset=US-ASCII 11, 29 -- Content-transfer-encoding: 7BIT 11, 29 -- Content-description: Mail message body 11, 29 -- X-Barracuda-Virus-Scanned: by Barracuda Spam Firewall at muohio.edu 11, 29 -- X-Barracuda-Spam-Score: 0.00 11, 29 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=3.5 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=1000.0 ==============================End of - Headers==============================
==============================Original Headers============================== 39, 39 -- From jcoleman-at-rdg.boehringer-ingelheim.com Thu Mar 8 07:49:04 2007 39, 39 -- Received: from rdg3v.boehringer-ingelheim.com (rdg3v.boehringer-ingelheim.com [148.188.144.50]) 39, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l28Dn3GB006945 39, 39 -- for {microscopy-at-Microscopy.com} ; Thu, 8 Mar 2007 07:49:03 -0600 39, 39 -- Received: from no.name.available by rdg3v.boehringer-ingelheim.com 39, 39 -- via smtpd (for microscopy.com [206.69.208.10]) with SMTP; Thu, 8 Mar 2007 09:01:30 -0500 39, 39 -- Received: from 148.189.144.34 by RDGES01.am.boehringer.com with ESMTP ( 39, 39 -- SMTP Relay); Thu, 08 Mar 2007 08:48:10 -0500 39, 39 -- X-Server-Uuid: 703CFCED-3C12-4D6E-8600-87AEF438FF4D 39, 39 -- Received: from RDGEXB01.am.boehringer.com ([148.189.116.104]) by 39, 39 -- RDGEXH01.boehringer.com with Microsoft SMTPSVC(6.0.3790.1830); Thu, 8 39, 39 -- Mar 2007 08:48:10 -0500 39, 39 -- Received: from RDGEXM05.am.boehringer.com ([148.189.119.245]) by 39, 39 -- RDGEXB01.am.boehringer.com with Microsoft SMTPSVC(6.0.3790.1830); Thu, 39, 39 -- 8 Mar 2007 08:48:09 -0500 39, 39 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 39, 39 -- Content-class: urn:content-classes:message 39, 39 -- MIME-Version: 1.0 39, 39 -- Subject: FW: [Microscopy] EM Field sources 39, 39 -- Date: Thu, 8 Mar 2007 08:48:09 -0500 39, 39 -- Message-ID: {EAB939ABFC28BB478C1E48035CAD851AC667BC-at-RDGEXM05.am.boehringer.com} 39, 39 -- X-MS-Has-Attach: 39, 39 -- X-MS-TNEF-Correlator: 39, 39 -- Thread-Topic: [Microscopy] EM Field sources 39, 39 -- Thread-Index: Acdg5NHmelv4aHgnS6mNjMghBanvVAAk7lQwAABzZZAAA2hkMA== 39, 39 -- From: jcoleman-at-rdg.boehringer-ingelheim.com 39, 39 -- To: microscopy-at-Microscopy.com 39, 39 -- X-OriginalArrivalTime: 08 Mar 2007 13:48:09.0773 (UTC) 39, 39 -- FILETIME=[685D25D0:01C76188] 39, 39 -- X-TMWD-Spam-Summary: TS=20070308134813; SEV=2.2.0; DFV=B2007030806; 39, 39 -- IFV=2.0.4,4.0-9; AIF=B2007030806; RPD=5.02.0004; ENG=IBF; 39, 39 -- RPDID=7374723D303030312E30413031303230372E34354630313431432E303133392C73733D312C6667733D30; 39, 39 -- CAT=NONE; CON=NONE 39, 39 -- X-MMS-Spam-Filter-ID: B2007030806_5.02.0004_4.0-9 39, 39 -- X-WSS-ID: 69EECB904501226357-01-01 39, 39 -- Content-Type: text/plain; 39, 39 -- charset=us-ascii 39, 39 -- Content-Transfer-Encoding: 8bit 39, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l28Dn3GB006945 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both martini-at-accurel.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: martini-at-accurel.com Name: Martin Izquierdo
We've had to fight field issues in an old building. We've found that most of our fields can be accounted for by someone making a ground-neutral bond, i.e. tying a neutral to a ground line. This, while it is low voltage, can generate large currents and hence large fields. Neutral-ground bonds are against the electrical code but they still occur.
I can recommend a hand-held gaussmeter which is good to 0.01mG. We got ours from http://www.lessemf.com/combi.html. Scroll down to the model A480. $169 (just a satisfied customer)
It is a single axis meter which we have found to be more useful in tracking down stray fields than a tri-axial unit. It has been indispensable, particularly at the price.
Good luck, Henk
At 01:13 PM 03/07/07, edelmare-at-muohio.edu wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 11, 26 -- From colijn.1-at-osu.edu Thu Mar 8 08:22:48 2007 11, 26 -- Received: from ER6S1.ENG.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l28EMmGn030265 11, 26 -- for {microscopy-at-microscopy.com} ; Thu, 8 Mar 2007 08:22:48 -0600 11, 26 -- Received: from CONVERSION-DAEMON.er6s1.eng.ohio-state.edu by 11, 26 -- er6s1.eng.ohio-state.edu (PMDF V6.2-1x11 #31056) 11, 26 -- id {01MDYYXQJ9UOAA9N3S-at-er6s1.eng.ohio-state.edu} for 11, 26 -- microscopy-at-microscopy.com; Thu, 08 Mar 2007 09:22:47 -0500 (EST) 11, 26 -- Received: from HOC1.ecr6.ohio-state.edu 11, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.eng.ohio-state.edu 11, 26 -- (PMDF V6.2-1x11 #31056) 11, 26 -- with ESMTPA id {01MDYYXQ4C26AA7H8P-at-er6s1.eng.ohio-state.edu} ; Thu, 11, 26 -- 08 Mar 2007 09:22:47 -0500 (EST) 11, 26 -- Date: Thu, 08 Mar 2007 09:25:55 -0500 11, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 11, 26 -- Subject: Re: [Microscopy] EM Field sources 11, 26 -- In-reply-to: {200703071813.l27IDxh8027478-at-ns.microscopy.com} 11, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 11, 26 -- To: edelmare-at-muohio.edu 11, 26 -- Cc: microscopy-at-microscopy.com 11, 26 -- Message-id: {7.0.1.0.2.20070308090804.03704a68-at-osu.edu} 11, 26 -- MIME-version: 1.0 11, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 11, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 11, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 11, 26 -- References: {200703071813.l27IDxh8027478-at-ns.microscopy.com} ==============================End of - Headers==============================
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Question: Hi, everyone I am a M.S student and I would like to study the bacterial cytoskeletal elements for various bacteria (E. coli for the moment). I would like to label FtsZ, MreB or such proteins with lectins and then label them with gold or directly label these proteins with gold-labelled lectins and then observe the results in TEM.
Does anyone know any specific lectins for bacterial cytoskeletal proteins or anything about this subject?
Any help is appreciated Thank you in advance
Ceren Buyukkayalar M.S student Gebze Institute of Technology Turkey
Richard; A cheap ( {$100), readily available handheld field meter like the Extech 480823, as recommended by Professor David Muller, will help you locate the source of the fields. http://www.extech.com/instrument/products/451_499/480823.html
John Mardinly Intel Corporation
This comment does not represent an opinion of Intel Corporation.
-----Original Message----- X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu] Sent: Wednesday, March 07, 2007 10:12 AM To: Mardinly, John
O.k., I'm looking for some help in understanding electro-magnetic fields, and the sources generating them.
Here's the issue in my lab we've gone from 0.46mG to 8.7mG in X-axis EMF-AC, and 1.1mG to 15.2mG in Z-axis EMF-AC. These obviously push beyond specs for the SEM in the room.
So I am looking for an education. I have no idea of the context to put this in. What would or could cause these kinds of EM Field increases (15 to 20 times)? A single new 110V outlet? A new 1000W UPS in a near by room? A 480V feeder line? A new HVAC blower motor?
These were measurements were made in the middle of a 10ft x 10ft x 9ft room (3.2m x 3.2m x 3m) Next to an SEM, in the Off state (no power). I operate the rooms on two sides and below, and I have checked the labs on the other lateral sides and the floor above - no new equipment or significant electrical services within at least 15 to 50 feet in these spaces. BUT "signifcant" what is significant? Am I looking for a new 110v outlet? or a computer UPS? Or am I looking for something in the next building? Or 150feet down the hall? I have not dug through the ceilings yet to look for any "hidden" changes but again I do not know what I should be looking for.
Am I looking for an induced current coming through an eithernet cable?
I do not have a hand held gauss-meter to track things down. Do I need one?
Any help would be great. Thanks folks.
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"A main-frame: The biggest PC peripheral available."
==============================Original Headers============================== 11, 29 -- From edelmare-at-muohio.edu Wed Mar 7 12:11:44 2007 11, 29 -- Received: from spamfirewall.muohio.edu (wallaby.mcs.muohio.edu [134.53.6.28]) 11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l27IBiOK024783 11, 29 -- for {microscopy-at-Microscopy.com} ; Wed, 7 Mar 2007 12:11:44 -0600 11, 29 -- X-ASG-Debug-ID: 1173291103-52a900520000-Dem1zR 11, 29 -- X-Barracuda-URL: http://134.53.6.28:80/cgi-bin/mark.cgi 11, 29 -- X-Barracuda-Connect: mulnx23.mcs.muohio.edu[134.53.6.10] 11, 29 -- X-Barracuda-Start-Time: 1173291103 11, 29 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 11, 29 -- by spamfirewall.muohio.edu (Spam Firewall) with ESMTP id 94100494B7 11, 29 -- for {microscopy-at-Microscopy.com} ; Wed, 7 Mar 2007 13:11:43 -0500 (EST) 11, 29 -- Received: from [192.168.1.23] ([134.53.14.105]) 11, 29 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l27IBhLq020342 11, 29 -- for {microscopy-at-Microscopy.com} ; Wed, 7 Mar 2007 13:11:43 -0500 11, 29 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 11, 29 -- To: microscopy-at-Microscopy.com 11, 29 -- Date: Wed, 07 Mar 2007 13:11:43 -0500 11, 29 -- MIME-Version: 1.0 11, 29 -- X-ASG-Orig-Subj: EM Field sources 11, 29 -- Subject: EM Field sources 11, 29 -- Message-ID: {45EEBA0F.27061.1EA342A-at-edelmare.muohio.edu} 11, 29 -- Priority: normal 11, 29 -- X-mailer: Pegasus Mail for Windows (4.41) 11, 29 -- Content-type: text/plain; charset=US-ASCII 11, 29 -- Content-transfer-encoding: 7BIT 11, 29 -- Content-description: Mail message body 11, 29 -- X-Barracuda-Virus-Scanned: by Barracuda Spam Firewall at muohio.edu 11, 29 -- X-Barracuda-Spam-Score: 0.00 11, 29 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=3.5 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=1000.0 ==============================End of - Headers==============================
==============================Original Headers============================== 21, 34 -- From john.mardinly-at-intel.com Thu Mar 8 10:51:46 2007 21, 34 -- Received: from mga02.intel.com (mga02.intel.com [134.134.136.20]) 21, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l28Gpkiq023469 21, 34 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 8 Mar 2007 10:51:46 -0600 21, 34 -- Received: from orsmga001.jf.intel.com ([10.7.209.18]) 21, 34 -- by mga02.intel.com with ESMTP; 08 Mar 2007 08:51:38 -0800 21, 34 -- Received: from fmsmsx334.amr.corp.intel.com ([132.233.42.1]) 21, 34 -- by orsmga001.jf.intel.com with ESMTP; 08 Mar 2007 08:51:38 -0800 21, 34 -- X-ExtLoop1: 1 21, 34 -- X-IronPort-AV: i="4.14,264,1170662400"; 21, 34 -- d="scan'208"; a="206074648:sNHT138287702" 21, 34 -- Received: from scsmsx411.amr.corp.intel.com ([10.3.90.30]) by fmsmsx334.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 21, 34 -- Thu, 8 Mar 2007 08:51:34 -0800 21, 34 -- Received: from scsmsx415.amr.corp.intel.com ([10.3.90.34]) by scsmsx411.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 21, 34 -- Thu, 8 Mar 2007 08:51:34 -0800 21, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 21, 34 -- Content-class: urn:content-classes:message 21, 34 -- MIME-Version: 1.0 21, 34 -- Content-Type: text/plain; 21, 34 -- charset="us-ascii" 21, 34 -- Subject: RE: [Microscopy] EM Field sources 21, 34 -- Date: Thu, 8 Mar 2007 08:51:33 -0800 21, 34 -- Message-ID: {F3CB8931ABF8294DB889E977150CAD6811A60A-at-scsmsx415.amr.corp.intel.com} 21, 34 -- In-Reply-To: {200703071811.l27IBnJH024910-at-ns.microscopy.com} 21, 34 -- X-MS-Has-Attach: 21, 34 -- X-MS-TNEF-Correlator: 21, 34 -- Thread-Topic: [Microscopy] EM Field sources 21, 34 -- thread-index: Acdg5CAa8oSLKGHSR1ufXM3otX3IHAAvUqAA 21, 34 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 21, 34 -- To: {edelmare-at-muohio.edu} 21, 34 -- Cc: {Microscopy-at-msa.microscopy.com} 21, 34 -- X-OriginalArrivalTime: 08 Mar 2007 16:51:34.0049 (UTC) FILETIME=[076C6910:01C761A2] 21, 34 -- Content-Transfer-Encoding: 8bit 21, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l28Gpkiq023469 ==============================End of - Headers==============================
If you what you mean by "analysis of paper" is a full 3D structure, the best I have ever seen was done by a company offering a proprietary service where serial sections are imaged on the blockface. I have no commercial interest and am not endorsing them in any way.
http://www.microsciencegroup.com/applications_publications.htm This page links to their applications papers. Select the one called "Filtration + Separation" for a Sept 2001 publication showing an example of filter paper. [I was surprised to see they also had a link to a Newsweek story in which I was interviewed.]
I, and many others, have had various degrees of success cutting and registering serial microtome sections of embedded paper. My preferred current method is SEM imaging after cross sections are prepared by embedding, polishing, and etching. It is illustrated in my abstract in the 2002 MSA Annual Meeting, Page 178. A key reference for the method is G.J. Williams and J.G. Drummond, J. Pulp and Paper Science, V26 (2000), P. 188
Final note. Surface structure is very well characterized by some of the modern white light interferometers. No preparation needed.
David R. Rothbard, Ph.D. BEP
-----Original Message----- X-from: greta.rennings-at-web.de To: rothbardd-at-netscape.net Sent: Wed, 7 Mar 2007 5:40 PM
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (greta.rennings-at-web.de) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, March 7, 2007 at 13:41:04 ------------------------------------------------------------------------- --
Email: greta.rennings-at-web.de Name: Greta Rennings
Organization: KU Leuven
Education: Graduate College
Location: Leuven, Brabant, Belgium
Question: Dear ladies and gentlemen,
do you have an idea which microscopic technique would be suitable for analysis of paper? Firstly for 3D I did trials with CLSM, which were not truly successful as grey scale differences were too low. Other techniques I know would require splitting or sectioning, as far as I know- do you have further experience? Secondly for 2D I tried light microscopy and SEM, but grey scale differences were to low here too in order to differentiate between components. Could TEM be useful? What could help to get better distinguishable features?
Thank you very much in advance for your support! Kind regards, Greta
Both CMOS and CCD sensors convert light into electrical signals that can be understood and interpreted by a computer, and they both use the same physical effects for the conversion, so the underlying physical limitations are the same. The difference is the technology used:
In a CCD chip, all the collected information in the many pixels of the chip are processed by a very limited number of electronic elements (voltage amplifier, etc.), often only one. While that can be a drawback in terms of speed, it does help with the uniformity of the signal. Plus, the individual pixels of the chip can be almost entirely used for converting photons to electrons, i.e., the CCD chips use almost 100% of the available area as light sensitive areas. For even higher sensitivity one can thin down the chips and illuminate them from the back side.
A CMOS (Complementary Metal-Oxide Semiconductor) chip uses a different technology for the fabrication. It is actually the same process that is used for regular electronics, so it is easy to also integrate some electronic devices on the chip. In a CMOS chip, each pixel has its own voltage amplifier and perhaps other electronics. In comparison to a CCD chip then, the uniformity is usually worse (many amplifiers as opposed to one), and the sensitivity is not as good (some "dead area" for each pixel). On the other hand, CMOS chips can integrate other electronics on the same chip ("camera on a chip"), and they can be much more energy efficient (hence their use in consumer type cameras that depend on batteries). The fact that the electronic circuits on each pixel eat up some space made CMOS quite useless for light sensitive chips until a few years ago, when the dimensions of those electronic circuits had shrunk to a size that allowed a significant area of each pixel to be used as light sensitive area.
Factors like resolution are not quite as simple as they appear. You can make cameras with really small pixels, but what happens is that the number of electrons you can store in each pixel becomes smaller and smaller. Comparing this number to the spontaneous generation of electrons (noise), you can see that the dynamic range of the camera gets reduced. For example, if you can store 100,000 electrons, and your noise level is 50 electrons, you can distinguish 2000 intensity levels, or about 11 bits. If your storage size shrinks to 10,000 electrons, you end up with 200 levels, or less than 8 bit. On the other hand, if you increase the pixel size, it takes longer to read them out, and the chip gets a lot bigger (=$$). But this applies to both CMOS and CCD. So, for any given set of parameters there is a different chip that is best. That could be a CMOS or CCD chip.
My personal opinion is that we will see more development in the CMOS sector (due to their use in consumer cameras), and they will start to eat into the CCD market. But I don't think that the CCD market will totally go away, especially in the scientific arena, where it is often necessary to squeeze some signal out of the last photon.
As far as durability goes, I don't see a reason why a CMOS chip should have a durability that is different from a CCD chip.
If you need more information, just google "CMOS CCD", and you'll get a lot of information that goes deeper than what I wrote up here.
Michael Bode, Ph.D.
General Manager
OLYMPUS SOFT IMAGING SOLUTIONS 12596 West Bayaud Ave #300 Lakewood, CO 80228 USA Tel.: +1 (303) 234-9270 Fax.: +1 (303) 234-9271 E-mail: Mike.Bode-at-olympus-sis.com www.olympus-sis.com
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: Thursday, March 08, 2007 13:11 To: Mike Bode
What are the advantages/disadvantages of CMOS compared to CCD cameras?
Are they comparable in terms of sensitivity, resolution and durability?
Thank you.
JB -- ############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Email: bozzola-at-siu.edu Web: http://www.siu.edu/~image/ ##############################################################
==============================Original Headers============================== 4, 18 -- From bozzola-at-siu.edu Thu Mar 8 14:05:50 2007 4, 18 -- Received: from abbmta2.siu.edu (abbmta2.siu.edu [131.230.254.206]) 4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l28K5oCA017235 4, 18 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 8 Mar 2007 14:05:50 -0600 4, 18 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) 4, 18 -- by abbmta2.siu.edu (Switch-3.1.11/Switch-3.1.11) with ESMTP id l28K5mJL017312 4, 18 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 8 Mar 2007 14:05:49 -0600 (CST) 4, 18 -- Mime-Version: 1.0 4, 18 -- X-Sender: bozzola-at-saluki-mail.siu.edu 4, 18 -- Message-Id: {p0611044cc2161c78b6b8-at-[131.230.177.142]} 4, 18 -- Date: Thu, 8 Mar 2007 14:05:44 -0600 4, 18 -- To: Microscopy-at-msa.microscopy.com 4, 18 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 4, 18 -- Subject: CMOS versus CCD cameras 4, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 18 -- X-Spam-Score: 0.00% 4, 18 -- X-MASF: 0.00% 4, 18 -- X-Whitelist: 0.00% ==============================End of - Headers==============================
==============================Original Headers============================== 22, 25 -- From Mike.Bode-at-olympus-sis.com Thu Mar 8 14:46:19 2007 22, 25 -- Received: from mail.soft-imaging.de (ns.soft-imaging.de [62.180.61.130]) 22, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l28KkJAg029215 22, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Mar 2007 14:46:19 -0600 22, 25 -- Received: from muenster.olympus-sis.com (muenster.olympus-sis.com [10.26.20.9]) 22, 25 -- by mail.soft-imaging.de (8.13.3/8.13.3/SuSE Linux 0.7) with ESMTP id l28KlEQm031653 22, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Mar 2007 21:47:17 +0100 22, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 22, 25 -- Content-class: urn:content-classes:message 22, 25 -- MIME-Version: 1.0 22, 25 -- Content-Type: text/plain; 22, 25 -- charset="us-ascii" 22, 25 -- Subject: RE: [Microscopy] CMOS versus CCD cameras 22, 25 -- Date: Thu, 8 Mar 2007 21:44:39 +0100 22, 25 -- Message-ID: {78B53BA1C5A2D9449EDA30A98800EC947FCD43-at-ms-s-gws.soft-imaging.net} 22, 25 -- In-Reply-To: {200703082011.l28KBLt6025602-at-ns.microscopy.com} 22, 25 -- X-MS-Has-Attach: 22, 25 -- X-MS-TNEF-Correlator: 22, 25 -- Thread-Topic: [Microscopy] CMOS versus CCD cameras 22, 25 -- Thread-Index: AcdhvfONo18KdfHKQAusDXJv1TstMwAAK7PA 22, 25 -- References: {200703082011.l28KBLt6025602-at-ns.microscopy.com} 22, 25 -- From: "Mike Bode" {Mike.Bode-at-olympus-sis.com} 22, 25 -- To: {Microscopy-at-microscopy.com} 22, 25 -- Content-Transfer-Encoding: 8bit 22, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l28KkJAg029215 ==============================End of - Headers==============================
Many thanks for the replies concerning digital imaging software. I received many replies, and also many requests to share the information I collected.
I also met up with a few people I have not contacted in a while and caught up on a bit of gossip. To all - please stay in contact.
Below is a condensed summary of the replies I received. I have removed identifiers so that contributors can remain anonymous.
I would say that Hitachi and Olympus (SIS) have been very good at supplying detail of the software they produce, and I thank them for that. I would suggest that anyone with specific interests in those software packages contact thir local people. I can also provide contact info if needed.
There is much more available than I imagined so the message is long.
Many thanks to eveyone who took the time to reply, and best wishes to you all.
Regards,
Paul
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
Summary: The quick answer is a database (ie Filemaker Pro Server, etc) but for a valadation product BioImagene Scientific Image Management System has a good product - BUT it costs. __________________________________________________________________________ Are you talking about film or digital images? If the latter, Leginon not only collects data automatically from a selection of targets, but it also archives all the data in a database--I think that feature alone is worth using Leginon for. The program is free, although someone from your lab will have to attend a training class (also free). Leginon requires pretty large computer resources, but if you are archiving a lot of images you will need that in any case. I am not connected with the purveyors (Clint Potter & Bridget Carrigher) of Leginon except as a satisfied user. __________________________________________________________________________ I think Quartz PCI software, http://www.qrtz.com/network.html ,may be just what you are looking for. __________________________________________________________________________ Extensis Portfolio 8 is one Strong system. I know several of our Histology / Pathology EM users have a hospital wide system that is very secure and very stable. The system is available for individual users, workgroups and as Client/Server solutions (but big $$ for the Bigger systems). __________________________________________________________________________ Extensis Portfolio 8 is one Strong system. I know several of our Histology / Pathology EM users have a hospital wide system that is very secure and very stable. The system is available for individual users, workgroups and as Client/Server solutions (but big $$ for the Bigger systems). __________________________________________________________________________ Aperio: http://www.aperio.com/productsservices/prod-spectrum.asp __________________________________________________________________________ Media Cybernetics' IQBase: http://www.mediacy.com/index.aspx?page=IQBase __________________________________________________________________________ Have you considered OME at http://www.openmicroscopy.org/ __________________________________________________________________________ I would contact: OLYMPUS SOFT IMAGING SOLUTIONS 12596 West Bayaud Ave #300 Lakewood, CO 80228 USA Tel.: +1 (303) 234-9270 Fax.: +1 (303) 234-9271 E-mail: Mike.Bode-at-olympus-sis.com Or Jason Wickersham {Jason.Wickersham-at-olympus-sis.com} www.olympus-sis.com __________________________________________________________________________ see http://www.cerious.com {http://www.cerious.com/} . ThumbsPlus should do what you want - if not you may need to go to something like Image Central - http://www.AIC-ImageCentral.com __________________________________________________________________________
You're after "digital asset management software," yes, DAM for short You can google the term to get a bunch of vendors. You really don't need to have your IT department develop the software for you. In addition, most vendors will let you try out their product for 2 weeks or so before you buy - I strongly recommend simultaneously trying out several so that you can compare them.
CanvasX may be what you need. The price is certainly right (~$600). I don't have experience with it, but it's aimed at a technical work group market, people doing technical drawings, that sort of thing, from what I see, it looks like the images can be extensively annotated. You want to see how many sites can upload images; I suspect it's only one. It's OK if it's all within one lab, but difficult if several labs want to load images to the same site. If you're considering putting up videos in addition to still images, you want to make sure it can handle them. Check it out at: http://www.acdamerica.com/
Image folio looks like it's aimed at a commercial market (ie, companies selling images), but it may suit your needs, with the same things to look for as with CanvasX - I wasn't convinced that this package offers much in the way of annotation capability (there are 3 packages, from $89 to $750): http://imagefolio.com/
We're using Contentdm (see our site http://cellimages.ascb.org/) - $10,000 initial license for up to 10,000 images, annual maintenance of ~ $2,000, with lots of tools and considerable flexibility, you can have password controlled access, intended for display over the Internet: http://www.dimema.com/ If you have this much $$$, you could create collections for every user. The license comes with 50 "Acquisitions Stations," which I believe means that you can have 50 computers with the capability to upload images. Take a look at our site, we've configured it to handle video as well as still images. You can also put together what they call "compound objects" - it's a way of grouping related items (eg, all the images from a given experiment) so that they all come up together. We can certainly help you out if you decide to go this route.
This is a nice grouping of a number of other DAM systems: http://web.reed.edu/digital_asset_mgmt/systems.html
==============================Original Headers============================== 18, 18 -- From PWebster-at-hei.org Thu Mar 8 14:52:18 2007 18, 18 -- Received: from hi0sml1.hei.org (House-Ear-Institute-1114861.cust-rtr.pacbell.net [71.136.31.242]) 18, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l28KqGBu008148 18, 18 -- for {Microscopy-at-Microscopy.Com} ; Thu, 8 Mar 2007 14:52:17 -0600 18, 18 -- Received: from 10.10.42.96 ([10.10.42.96]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 18, 18 -- Thu, 8 Mar 2007 20:52:15 +0000 18, 18 -- User-Agent: Microsoft-Entourage/11.3.3.061214 18, 18 -- Date: Thu, 08 Mar 2007 12:52:15 -0800 18, 18 -- Subject: RE; EM & LM Digital database 18, 18 -- From: "Webster, Paul" {PWebster-at-hei.org} 18, 18 -- To: "microscopy-at-microscopy.com" {Microscopy-at-Microscopy.Com} 18, 18 -- Message-ID: {C215B77F.FCB5%PWebster-at-hei.org} 18, 18 -- Thread-Topic: RE; EM & LM Digital database 18, 18 -- Thread-Index: Acdhw6bm5YngKs22EduUUwANk7Zh7g== 18, 18 -- Mime-version: 1.0 18, 18 -- Content-type: text/plain; 18, 18 -- charset="US-ASCII" 18, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
does discuss noise. Both links discuss the added external circuitry needed to read out the analog data from CCD. But having on-chip A/D increases chip size and also puts pressure on being able to reduce pixel dimensions. The recent availability of smaller feature size CMOS helps in this respect. It also helps with off-chip signal processing which both sensors have to have.
The choice depends on application and target price. For imaging satellites, CCD is the choice. So too for high quality telescope cameras (Peltier cooled). To my knowledge, no one has made a cooled CMOS sensor. CMOS has inherently more shot and thermal noise than CCD. This does not really affect the consumer market. But for professional users, their cameras use CCD. The use of CMOS by Canon in an SLR is a first and will be interesting to follow.
Hope this helps.
gary g.
At 12:07 PM 3/8/2007, you wrote:
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==============================Original Headers============================== 18, 20 -- From gary-at-gaugler.com Thu Mar 8 14:59:08 2007 18, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 18, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l28Kx7J8019672 18, 20 -- for {microscopy-at-microscopy.com} ; Thu, 8 Mar 2007 14:59:08 -0600 18, 20 -- Message-Id: {200703082059.l28Kx7J8019672-at-ns.microscopy.com} 18, 20 -- Received: (qmail 25268 invoked from network); 8 Mar 2007 12:52:25 -0800 18, 20 -- Received: by simscan 1.1.0 ppid: 25262, pid: 25264, t: 0.1032s 18, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 18, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 18, 20 -- by qsmtp4 with SMTP; 8 Mar 2007 12:52:24 -0800 18, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 18, 20 -- Date: Thu, 08 Mar 2007 12:57:08 -0800 18, 20 -- To: bozzola-at-siu.edu 18, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 18, 20 -- Subject: Re: [Microscopy] CMOS versus CCD cameras 18, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 18, 20 -- In-Reply-To: {200703082007.l28K7mHn020146-at-ns.microscopy.com} 18, 20 -- References: {200703082007.l28K7mHn020146-at-ns.microscopy.com} 18, 20 -- Mime-Version: 1.0 18, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-47706DF4 ==============================End of - Headers==============================
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Email: camiller-at-anatomy.iupui.edu Name: Caroline Miller
Organization: Indiana Microscopy Society
Title-Subject: [Filtered] reminder of abstract deadline for joint LAS meeting
Question: A reminder of abstract deadline for the Joint LAS spring meeting, "Imaging for Nanotechnology," being held in Indianapolis on April 20th and 21st. Deadline has been extended to March 15th.
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Email: javaidqazi-at-kemet.com Name: Javaid Qazi
Title-Subject: [Filtered] OM Calibration standard Z direction
Question: I am looking for a Z direction Calibration standard for an optical microscope which can do surface profiles, mag ranges from 20 to 1000x.
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Organization: Electron Microscopy Program (Delta College)
Title-Subject: [Filtered] EDS/WDS systems
Question: Hello all! I am doing a presentation on EDS and WDS systems and I can't seem to find any information on how much these systems would cost, ready for installation on any TEM/SEM. I understand that there are various types of detector systems..I would just like a ballpark estimate on what these systems might cost. Any information would be greatly appreciated. Thank you
A colleague recently told me that a carbon evaporation coater (for sem/epma samples) now lists for over ~US$55K (full scale model-not tabletop, with diffusion pump, no thickness monitor). This is essentially the same unit that I purchased 13 years ago for ~$12K.
Does anyone have any idea why the price for such a unit has increased by } 400% in 13 years? Are there still "simple full scale" (not tabletop) models that sell at more "reasonable" prices? (He asked me if I thought an NSF proposal reviewer would look askance as such a high cost of a coater in a proposal...)
Thanks.
John -- ======================================================== John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480 Cameron Electron Microprobe Lab lab: (608) 265-4798 Dept of Geology & Geophysics fax: (608) 262-0693 University of Wisconsin home: (608) 274-2245 1215 West Dayton St. email: johnf-at-geology.wisc.edu Madison, WI 53706 amateur radio: WA3BTA Personal http://www.geology.wisc.edu/~johnf/ Probe lab http://www.geology.wisc.edu/~johnf/sx51.html Probe Sign Up Calender: http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi
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==============================Original Headers============================== 6, 24 -- From johnf-at-geology.wisc.edu Fri Mar 9 09:31:20 2007 6, 24 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l29FVKUv021817 6, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 Mar 2007 09:31:20 -0600 6, 24 -- Received: from localhost (localhost [127.0.0.1]) 6, 24 -- by localhost (Postfix) with ESMTP id F257A20D10 6, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 Mar 2007 09:31:19 -0600 (CST) 6, 24 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) 6, 24 -- by localhost (geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 6, 24 -- with ESMTP id 24815-03-5 for {microscopy-at-microscopy.com} ; 6, 24 -- Fri, 9 Mar 2007 09:31:08 -0600 (CST) 6, 24 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57]) 6, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 6, 24 -- (No client certificate requested) 6, 24 -- by ice.geology.wisc.edu (Postfix) with ESMTP id E6A9220D04 6, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 Mar 2007 09:30:50 -0600 (CST) 6, 24 -- Mime-Version: 1.0 6, 24 -- Message-Id: {p06230905c2172cf20092-at-[144.92.206.57]} 6, 24 -- Date: Fri, 9 Mar 2007 09:30:47 -0600 6, 24 -- To: microscopy-at-microscopy.com 6, 24 -- From: John Fournelle {johnf-at-geology.wisc.edu} 6, 24 -- Subject: Carbon evaporation coaters 6, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 6, 24 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu ==============================End of - Headers==============================
Dr. Godfrey Mbah, faculty at a local college needs to use a Jumbo Supercritical Point Dryer for his research. If anyone has one within driving distance from Columbia, SC, please contact him directly. He is willing to pay for use of the instrument. Godfrey can be reached at Mbahg-at-benedict.edu.
Thanks for your help.
Soumitra
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On Mar 9, 2007, at 7:31 AM, johnf-at-geology.wisc.edu wrote:
} A colleague recently told me that a carbon evaporation coater (for } sem/epma samples) now lists for over ~US$55K (full scale model-not } tabletop, with diffusion pump, no thickness monitor). This is } essentially the same unit that I purchased 13 years ago for ~$12K. } } Does anyone have any idea why the price for such a unit has increased } by } 400% in 13 years? Are there still "simple full scale" (not } tabletop) models that sell at more "reasonable" prices? (He asked me } if I thought an NSF proposal reviewer would look askance as such a } high cost of a coater in a proposal...) } Hi John, About 3 years ago I got a Cressington 208 evaporation system (both carbon and the optional metal power supplies) from Pella, and have been quite satisfied. The cost was about half your quote (but may have increased). The web site is:
http://www.tedpella.com/cressing_html/intro.html
I have no relationship to Pella except as a satisfied user. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Fri Mar 9 13:12:58 2007 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l29JCwP3016811 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Mar 2007 13:12:58 -0600 6, 22 -- Received: from water-dog.caltech.edu (water-dog [192.168.1.26]) 6, 22 -- by wood-ox-postvirus (Postfix) with ESMTP id 8CAFA2EF07 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Mar 2007 11:12:57 -0800 (PST) 6, 22 -- Received: from [192.168.159.233] (jpix-01.caltech.edu [131.215.2.133]) 6, 22 -- by wood-ox.its.caltech.edu (Postfix) with ESMTP id 2D3B72EF05 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Mar 2007 11:12:54 -0800 (PST) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v624) 6, 22 -- In-Reply-To: {200703091531.l29FVUdA021972-at-ns.microscopy.com} 6, 22 -- References: {200703091531.l29FVUdA021972-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 6, 22 -- Message-Id: {fbb1aa8772195af89212fb367271a0ff-at-caltech.edu} 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- Subject: Re: [Microscopy] Carbon evaporation coaters 6, 22 -- Date: Fri, 9 Mar 2007 11:22:18 -0800 6, 22 -- To: microscopy-at-msa.microscopy.com 6, 22 -- X-Mailer: Apple Mail (2.624) 6, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.3.3 ==============================End of - Headers==============================
Check out Ladd Research for a free standing evaporator for a lot less than $55K. http://www.laddresearch.com
FYI: Ladd Research was the only manufacturer or supplier of a rubber L gasket with a large lip on the bottom to hold the older and thicker walled glass bell jars. I tried 5-8 suppliers. They also supplied me with custom made leads on their dual evaporation unit for retrofitting to my Cooke thermal boat evaporator. I would ask any supplier about carbon tread flash evaporations and/or accessories.
Disclaimer: I am just a satified customer that got great customer support and products.
Paul
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Dear Javaid, The method I know of for calibrating the fine focus knob of the optical microscope is to measure the thickness of a microscope glass slide with a fine micrometer, put a mark with felt pen on both sides of the slide, offset from each other a bit, then record the fine focus reading for the focus on one mark and the fine focus reading for the other mark. Do this for each objective. The calibration is then based on the micrometer you use. Hope this helps. Regards,
-----Original Message----- X-from: javaidqazi-at-kemet.com [mailto:javaidqazi-at-kemet.com] Sent: March 8, 2007 3:43 PM To: mager-at-interchange.ubc.ca
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Email: javaidqazi-at-kemet.com Name: Javaid Qazi
Title-Subject: [Filtered] OM Calibration standard Z direction
Question: I am looking for a Z direction Calibration standard for an optical microscope which can do surface profiles, mag ranges from 20 to 1000x.
Are you taking into account index of refraction of the glass, while measuring fine focus of the marks on both sides of the slide? My guess is that accuracy of such calibration will depend not only on micrometer, but also on how accurately the index of refraction is known; it should be close to 1.2 - 1.5 but probably will vary depending on source of the glass, etc.
You probably could use height standards made for AFM, some even NIST traceable, there are lots of sources, just Google. Of cause the accuracy of calibration will still depend on the operator...
Cheers, Valery
-----Original Message----- X-from: mager-at-interchange.ubc.ca [mailto:mager-at-interchange.ubc.ca] Sent: Friday, March 09, 2007 3:04 PM To: vray-at-partbeamsystech.com
Dear Javaid, The method I know of for calibrating the fine focus knob of the optical microscope is to measure the thickness of a microscope glass slide with a fine micrometer, put a mark with felt pen on both sides of the slide, offset from each other a bit, then record the fine focus reading for the focus on one mark and the fine focus reading for the other mark. Do this for each objective. The calibration is then based on the micrometer you use. Hope this helps. Regards,
-----Original Message----- X-from: javaidqazi-at-kemet.com [mailto:javaidqazi-at-kemet.com] Sent: March 8, 2007 3:43 PM To: mager-at-interchange.ubc.ca
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Email: javaidqazi-at-kemet.com Name: Javaid Qazi
Title-Subject: [Filtered] OM Calibration standard Z direction
Question: I am looking for a Z direction Calibration standard for an optical microscope which can do surface profiles, mag ranges from 20 to 1000x.
as Steve points out the OL is turned off in LOW MAG mode. In this case you use the SAD aperture for contrast not the OL aperture.
Low mag is one of the strengths of this machine.
In Mag Mode if you cannot get good images below 100k you should use different alpha angles, alpha 3 for the low end and alpha 1 for high mag, high resolution. This allows you to control beam divergence to suit the magnification,
Best Regards
Richard /_____________________________________________________________________________________/ // /Richard Hey Principal Technical Support Engineer/ /Jeol (UK) Ltd/ // /Phone: +44 1707377117/ /Fax: +44 1707373255/
protrain-at-emcourses.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi } } I have not used the JEOL 2011 but it is a TEM so it must follow standard TEM } principles. } } Instruments usually switch off the objective lens to achieve very low } magnifications. They use the diffraction lens to focus and gain some } contrast through the inclusion of the intermediate or diffraction aperture. } } Try switching off the objective (some drop to 20% rather then switch off) } and use the diffraction lens to focus. Balance the remaining lenses to } reduce distortion if this arrangement causes problems. Introduce the } diffraction aperture once you have a reasonable image. } } The image quality will not be good, probably in excess of 3nm resolution, } due to the very long focal length required. } } Best of luck but if you need more help I am only in Buckingham? } } Steve Chapman } Protrain for EM training & consultancy world wide } www.emcourses.com } Tel +44 1280 816512 Fax +44 1280 814007 } Mobile +44 7711 606967 } } } } } ----- Original Message ----- } X-from: {richard.beanland-at-bookham.com} } To: {protrain-at-emcourses.com} } Sent: Wednesday, February 21, 2007 11:56 AM } Subject: [Microscopy] TEM: Free lens control on a JEOL 2011 } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear list readers, } } Having struggled with my 'new' microscope for a few years now I } } am reaching the limits of my knowledge. I am looking at relatively } } large GaAs devices (} 100um in diameter) and need to be able to take } } diffraction contrast images of the whole thing. The 2011 is great at } } magnifications } 100,000x but if I try to get an image at 100x all I see } } is a tiny bright spot corresponding to the objective aperture. I } } suspect this means that the objective aperture is nowhere near the back } } focal plane of the objective lens in low mag mode. Now, I know the kind } } of image I want was easy to get on my 1979 vintage 120CX, with a 2-stage } } condenser and one objective lens, whereas this beast has a three stage } } condenser plus a condenser and objective mini-lenses. } } This morning I managed to get a reasonable low mag diffraction } } contrast image by playing with the free lens controls, (essentially } } turning off some lenses so it behaved more like my old machine). My } } question is: has anyone done this in a more systematic manner and could } } give me some directions on which lenses to vary to get what I want? I } } could just about work it out myself with 3 lenses but I have no idea } } when there are 5. Not to mention 2 more in the gun, 3 intermediates and } } a projector, plus alignment lenses... } } } } Many TIA } } } } Richard } } } } ________________________________________ } } Richard Beanland } } Materials Analysis } } Bookham } } Caswell } } Towcester } } Northants } } NN12 8EQ } } United Kingdom } } Tel. +44 1327 356362 } } Fax. +44 1327 356775 } } http://www.bookham.com } } ________________________________________ } } } } } } ======================================================================= } } This e-mail is intended for the person it is addressed to only. The } } information contained in it may be confidential and/or protected by } } law. 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Any use, } } forwarding, printing or copying of this message is strictly prohibited. } } No part of this message can be considered a request for goods or } } services. } } ======================================================================= } } } } } } ==============================Original } } Headers============================== } } 6, 31 -- From richard.beanland-at-bookham.com Wed Feb 21 05:53:31 2007 } } 6, 31 -- Received: from mail115.messagelabs.com (mail115.messagelabs.com } } [195.245.231.179]) } } 6, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } } l1LBrSVJ010996 } } 6, 31 -- for {microscopy-at-microscopy.com} ; Wed, 21 Feb 2007 05:53:30 -0600 } } 6, 31 -- X-VirusChecked: Checked } } 6, 31 -- X-Env-Sender: richard.beanland-at-bookham.com } } 6, 31 -- X-Msg-Ref: } } server-14.tower-115.messagelabs.com!1172058777!30935280!1 } } 6, 31 -- X-StarScan-Version: 5.5.10.7.1; banners=bookham.com,-,- } } 6, 31 -- X-Originating-IP: [213.249.209.179] } } 6, 31 -- Received: (qmail 8028 invoked from network); 21 Feb 2007 } } 11:52:57 -0000 } } 6, 31 -- Received: from unknown (HELO } } cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) } } 6, 31 -- by server-14.tower-115.messagelabs.com with SMTP; 21 Feb 2007 } } 11:52:57 -0000 } } 6, 31 -- Received: from cas-smx-02.BOOKHAM.ENTERPRISE.PRI ([10.4.0.223]) } } by cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI with Microsoft } } SMTPSVC(6.0.3790.1830); } } 6, 31 -- Wed, 21 Feb 2007 11:54:43 +0000 } } 6, 31 -- Content-class: urn:content-classes:message } } 6, 31 -- MIME-Version: 1.0 } } 6, 31 -- Content-Type: text/plain; } } 6, 31 -- charset="us-ascii" } } 6, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } } 6, 31 -- Subject: TEM: Free lens control on a JEOL 2011 } } 6, 31 -- Date: Wed, 21 Feb 2007 11:54:42 -0000 } } 6, 31 -- Message-ID: } } {9645D3E33E4C6548B12A7B25F611533E3E8561-at-cas-smx-02.BOOKHAM.ENTERPRISE.PRI} } } 6, 31 -- X-MS-Has-Attach: } } 6, 31 -- X-MS-TNEF-Correlator: } } 6, 31 -- Thread-Topic: TEM: Free lens control on a JEOL 2011 } } 6, 31 -- Thread-Index: AcdVrxKD+5inJvYkRR6cl3TvhcEmkA== } } 6, 31 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} } } 6, 31 -- To: {microscopy-at-microscopy.com} } } 6, 31 -- X-OriginalArrivalTime: 21 Feb 2007 11:54:43.0238 (UTC) } } FILETIME=[13281460:01C755AF] } } 6, 31 -- Content-Transfer-Encoding: 8bit } } 6, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id l1LBrSVJ010996 } } ==============================End of - } } Headers============================== } } } } } } } } } } ==============================Original Headers============================== } 15, 27 -- From protrain-at-emcourses.com Wed Feb 21 13:34:56 2007 } 15, 27 -- Received: from smtp2.pri.skybb.uk.easynet.net (smtp2.pri.skybb.uk.easynet.net [87.86.189.70]) } 15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l1LJYs1v010369 } 15, 27 -- for {microscopy-at-microscopy.com} ; Wed, 21 Feb 2007 13:34:55 -0600 } 15, 27 -- Received: from 5ac27b43.bb.sky.com ([90.194.123.67] helo=HP6220) } 15, 27 -- by smtp2.pri.skybb.uk.easynet.net with smtp (Exim 4.62) } 15, 27 -- (envelope-from {protrain-at-emcourses.com} ) } 15, 27 -- id 1HJxEp-0003ph-10; Wed, 21 Feb 2007 19:34:52 +0000 } 15, 27 -- Message-ID: {001001c755ef$5a56c850$0200a8c0-at-HP6220} } 15, 27 -- Reply-To: "Steve Chapman" {protrain-at-emcourses.com} } 15, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com} } 15, 27 -- To: {richard.beanland-at-bookham.com} } 15, 27 -- Cc: "Microscopical Society America" {microscopy-at-microscopy.com} } 15, 27 -- References: {200702211156.l1LBuVPi012770-at-ns.microscopy.com} } 15, 27 -- Subject: Re: [Microscopy] TEM: Free lens control on a JEOL 2011 } 15, 27 -- Date: Wed, 21 Feb 2007 19:34:46 -0000 } 15, 27 -- Organization: Protrain } 15, 27 -- MIME-Version: 1.0 } 15, 27 -- Content-Type: text/plain; } 15, 27 -- format=flowed; } 15, 27 -- charset="iso-8859-1"; } 15, 27 -- reply-type=original } 15, 27 -- Content-Transfer-Encoding: 7bit } 15, 27 -- X-Priority: 3 } 15, 27 -- X-MSMail-Priority: Normal } 15, 27 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3028 } 15, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 } ==============================End of - Headers============================== } } }
==============================Original Headers============================== 6, 19 -- From richard-at-torland.demon.co.uk Sat Mar 10 06:22:29 2007 6, 19 -- Received: from anchor-post-31.mail.demon.net (anchor-post-31.mail.demon.net [194.217.242.89]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2ACMSMc006854 6, 19 -- for {microscopy-at-microscopy.com} ; Sat, 10 Mar 2007 06:22:29 -0600 6, 19 -- Received: from torland.demon.co.uk ([80.177.208.173] helo=[127.0.0.1]) 6, 19 -- by anchor-post-31.mail.demon.net with esmtp (Exim 4.42) 6, 19 -- id 1HQ0ah-000L2s-5V 6, 19 -- for microscopy-at-microscopy.com; Sat, 10 Mar 2007 12:22:27 +0000 6, 19 -- Message-ID: {45F2A303.4080400-at-torland.demon.co.uk} 6, 19 -- Date: Sat, 10 Mar 2007 12:22:27 +0000 6, 19 -- From: Richard {richard-at-torland.demon.co.uk} 6, 19 -- User-Agent: Thunderbird 1.5.0.10 (Windows/20070221) 6, 19 -- MIME-Version: 1.0 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- Subject: Re: TEM: Free lens control on a JEOL 2011 6, 19 -- References: {200702211937.l1LJbS9M015538-at-ns.microscopy.com} 6, 19 -- In-Reply-To: {200702211937.l1LJbS9M015538-at-ns.microscopy.com} 6, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Quick note of thanks to those who provided data and advice on power supply requirements for the HBO 50 Mercury burner. I now have enough info to build a power supply and am in the search mode for components.
Again, thanks for the help..
Best Regards,
Gene
==============================Original Headers============================== 6, 18 -- From W8KXR-at-neo.rr.com Sat Mar 10 07:05:35 2007 6, 18 -- Received: from ms-smtp-02.ohiordc.rr.com (ms-smtp-02.ohiordc.rr.com [65.24.5.136]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2AD5ZVZ028352 6, 18 -- for {Microscopy-at-microscopy.com} ; Sat, 10 Mar 2007 07:05:35 -0600 6, 18 -- Received: from [192.168.1.100] (cpe-75-179-26-108.neo.res.rr.com [75.179.26.108]) 6, 18 -- by ms-smtp-02.ohiordc.rr.com (8.13.6/8.13.6) with ESMTP id l2AD5YvP018636 6, 18 -- for {Microscopy-at-microscopy.com} ; Sat, 10 Mar 2007 08:05:34 -0500 (EST) 6, 18 -- Message-ID: {45F2AD1C.3000704-at-neo.rr.com} 6, 18 -- Date: Sat, 10 Mar 2007 08:05:32 -0500 6, 18 -- From: Gene Beckwith {W8KXR-at-neo.rr.com} 6, 18 -- Reply-To: W8KXR-at-neo.rr.com 6, 18 -- User-Agent: Thunderbird 1.5.0.10 (Windows/20070221) 6, 18 -- MIME-Version: 1.0 6, 18 -- To: Microscopy Reflector {Microscopy-at-microscopy.com} 6, 18 -- Subject: LM Mecury Burner data - Thanks - 6, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 18 -- Content-Transfer-Encoding: 7bit 6, 18 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
Duniway sells L-gaskets for Bell Jars. As does Kurt J. Lesker, NeVac, Huntington, Key High Vacuum, MDC, CalSeal, GreatGlass, etc.....
regards,
Jim
} From mail-at-ns.microscopy.com Fri Mar 9 14:55:36 2007 } Date: Fri, 9 Mar 2007 14:03:48 -0600 } To: jquinn-at-www.matscieng.sunysb.edu } From: beaurega-at-westol.com } Reply-to: beaurega-at-westol.com } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] Re: Carbon evaporation coaters } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, } } Check out Ladd Research for a free standing evaporator for a lot less than } $55K. } http://www.laddresearch.com } } FYI: Ladd Research was the only manufacturer or supplier of a rubber L } gasket with a large lip on the bottom to hold the older and thicker walled } glass bell jars. I tried 5-8 suppliers. } They also supplied me with custom made leads on their dual evaporation unit } for retrofitting to my Cooke thermal boat evaporator. I would ask any } supplier about carbon tread flash evaporations and/or accessories. } } Disclaimer: I am just a satified customer that got great customer support } and products. } } Paul } } At 09:32 AM 3/9/07 -0600, you wrote: } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } A colleague recently told me that a carbon evaporation coater (for } } sem/epma samples) now lists for over ~US$55K (full scale model-not } } tabletop, with diffusion pump, no thickness monitor). This is } } essentially the same unit that I purchased 13 years ago for ~$12K. } } } } Does anyone have any idea why the price for such a unit has increased } } by } 400% in 13 years? Are there still "simple full scale" (not } } tabletop) models that sell at more "reasonable" prices? (He asked me } } if I thought an NSF proposal reviewer would look askance as such a } } high cost of a coater in a proposal...) } } } } Thanks. } } } } John } } -- } } ======================================================== } } John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480 } } Cameron Electron Microprobe Lab lab: (608) 265-4798 } } Dept of Geology & Geophysics fax: (608) 262-0693 } } University of Wisconsin home: (608) 274-2245 } } 1215 West Dayton St. email: johnf-at-geology.wisc.edu } } Madison, WI 53706 amateur radio: WA3BTA } } Personal http://www.geology.wisc.edu/~johnf/ } } Probe lab http://www.geology.wisc.edu/~johnf/sx51.html } } Probe Sign Up Calender: } } http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi } } } } "The first rule of all intelligent tinkering is to save every cog and } } wheel." -- Aldo Leopold } } } } "For a successful technology, reality must take precedence over } } public relations, for Nature cannot be fooled." -- Richard P. } } Feynman } } } } ==============================Original Headers============================== } } 6, 24 -- From johnf-at-geology.wisc.edu Fri Mar 9 09:31:20 2007 } } 6, 24 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu } [144.92.206.14]) } } 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l29FVKUv021817 } } 6, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 Mar 2007 09:31:20 -0600 } } 6, 24 -- Received: from localhost (localhost [127.0.0.1]) } } 6, 24 -- by localhost (Postfix) with ESMTP id F257A20D10 } } 6, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 Mar 2007 09:31:19 -0600 } (CST) } } 6, 24 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) } } 6, 24 -- by localhost (geology.wisc.edu [127.0.0.1]) (amavisd-new, port } 10024) } } 6, 24 -- with ESMTP id 24815-03-5 for {microscopy-at-microscopy.com} ; } } 6, 24 -- Fri, 9 Mar 2007 09:31:08 -0600 (CST) } } 6, 24 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu } [144.92.206.57]) } } 6, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) } } 6, 24 -- (No client certificate requested) } } 6, 24 -- by ice.geology.wisc.edu (Postfix) with ESMTP id E6A9220D04 } } 6, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 Mar 2007 09:30:50 -0600 } (CST) } } 6, 24 -- Mime-Version: 1.0 } } 6, 24 -- Message-Id: {p06230905c2172cf20092-at-[144.92.206.57]} } } 6, 24 -- Date: Fri, 9 Mar 2007 09:30:47 -0600 } } 6, 24 -- To: microscopy-at-microscopy.com } } 6, 24 -- From: John Fournelle {johnf-at-geology.wisc.edu} } } 6, 24 -- Subject: Carbon evaporation coaters } } 6, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } } 6, 24 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu } } ==============================End of - Headers============================== } } } } } } } } ==============================Original Headers============================== } 5, 24 -- From beaurega-at-westol.com Fri Mar 9 14:02:50 2007 } 5, 24 -- Received: from smtp-gateway-5.winbeam.com (smtp-gateway-5.winbeam.com [64.84.97.70]) } 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l29K2oVE028755 } 5, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 Mar 2007 14:02:50 -0600 } 5, 24 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) } 5, 24 -- by smtp-gateway-5.winbeam.com (8.13.2/8.12.8) with SMTP id l29K0PrX005785 } 5, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 Mar 2007 15:00:26 -0500 } 5, 24 -- Received: (qmail 28598 invoked by uid 89); 9 Mar 2007 20:00:27 -0000 } 5, 24 -- Received: from pitts-69-72-117-129.dynamic-dialup.coretel.net (HELO millenium) (69.72.117.129) } 5, 24 -- by mail.winbeam.com with SMTP; 9 Mar 2007 20:00:27 -0000 } 5, 24 -- Message-Id: {3.0.6.32.20070309150108.007ee100-at-pop3.norton.antivirus} } 5, 24 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus } 5, 24 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) } 5, 24 -- Date: Fri, 09 Mar 2007 15:01:08 -0500 } 5, 24 -- To: johnf-at-geology.wisc.edu, microscopy-at-microscopy.com } 5, 24 -- From: Beaurega {beaurega-at-westol.com} } 5, 24 -- Subject: Re: [Microscopy] Carbon evaporation coaters } 5, 24 -- In-Reply-To: {200703091532.l29FW0Hq022583-at-ns.microscopy.com} } 5, 24 -- Mime-Version: 1.0 } 5, 24 -- Content-Type: text/plain; charset="us-ascii" } 5, 24 -- X--MailScanner-Information: - Please contact Technical Support for more information } 5, 24 -- X--MailScanner: Found to be clean (courtesy of MailScanner) } 5, 24 -- X--MailScanner-SpamCheck: not spam, SpamAssassin (not cached, timed out) } 5, 24 -- X--MailScanner-From: beaurega-at-westol.com } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 12 -- From jquinn-at-www.matscieng.sunysb.edu Sat Mar 10 14:06:16 2007 8, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2AK6F5h000308 8, 12 -- for {microscopy-at-microscopy.com} ; Sat, 10 Mar 2007 14:06:15 -0600 8, 12 -- Received: (from jquinn-at-localhost) 8, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id l2AJvnj09518; 8, 12 -- Sat, 10 Mar 2007 14:57:49 -0500 8, 12 -- Date: Sat, 10 Mar 2007 14:57:49 -0500 8, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 8, 12 -- Message-Id: {200703101957.l2AJvnj09518-at-www.matscieng.sunysb.edu} 8, 12 -- To: beaurega-at-westol.com, microscopy-at-microscopy.com 8, 12 -- Subject: Re: [Microscopy] Re: Carbon evaporation coaters ==============================End of - Headers==============================
I browsed with interest the links mentioned recently concerning digital camera adapters for photomicrography. On the vendor web pages, I see mentioned T-mount, C-mount, CS-mount etc. Can anyone point me to a source that defines the standard dimensions of various camera mounts such as these?
regards, Don Chernoff ================================== Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.]
==============================Original Headers============================== 4, 21 -- From donc-at-asmicro.com Sat Mar 10 22:15:16 2007 4, 21 -- Received: from smtp102.sbc.mail.mud.yahoo.com (smtp102.sbc.mail.mud.yahoo.com [68.142.198.201]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2B4FGbO017284 4, 21 -- for {microscopy-at-microscopy.com} ; Sat, 10 Mar 2007 22:15:16 -0600 4, 21 -- Received: (qmail 65445 invoked from network); 11 Mar 2007 04:15:21 -0000 4, 21 -- Received: from unknown (HELO ASM11) (asmicro-at-sbcglobal.net-at-68.57.205.80 with login) 4, 21 -- by smtp102.sbc.mail.mud.yahoo.com with SMTP; 11 Mar 2007 04:15:21 -0000 4, 21 -- X-YMail-OSG: 9QfmSEEVM1mbmoDSpk6B4Vk5UjNxazracQdSwvndGVowl5w3OHA9afZnedto1BgUyYj65NfW3wktWrFHZpCmmEFqcR1ycLl1Mll4GGdIADjodr6SFn0YjkplxDEIJYtbJMRS2tL_ntHE_a4- 4, 21 -- Message-ID: {006301c76393$8e29e0c0$3301a8c0-at-ASM11} 4, 21 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 4, 21 -- To: "Microscopy List" {microscopy-at-microscopy.com} 4, 21 -- Subject: T, C, CS and other mounts 4, 21 -- Date: Sat, 10 Mar 2007 23:11:24 -0500 4, 21 -- MIME-Version: 1.0 4, 21 -- Content-Type: text/plain; 4, 21 -- charset="iso-8859-1" 4, 21 -- Content-Transfer-Encoding: 7bit 4, 21 -- X-Priority: 3 4, 21 -- X-MSMail-Priority: Normal 4, 21 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1807 4, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1896 ==============================End of - Headers==============================
A colleague needs to label nucleoli in mouse thymus and so far I was not able to find a good antibody (tried a couple). I will be using Tokuyasu cryosections fixed with 6% formaldehyde. I would prefer commercially rabbit polyclonal antibody against something really abundant (fibrillarin, nucleolin or...?). Any tips?
Thanks,
Michal
==============================Original Headers============================== 5, 17 -- From Michal.Jarnik-at-fccc.edu Mon Mar 12 09:12:18 2007 5, 17 -- Received: from azah.fccc.edu (azah.fccc.edu [131.249.4.237]) 5, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2CECIQB021101 5, 17 -- for {microscopy-at-msa.microscopy.com} ; Mon, 12 Mar 2007 09:12:18 -0500 5, 17 -- Received: from [10.40.12.212] (emf1.dyn.fccc.edu [10.40.12.212]) 5, 17 -- (authenticated bits=0) 5, 17 -- by azah.fccc.edu (8.13.6/8.13.6) with ESMTP id l2CECDDl026992 5, 17 -- for {microscopy-at-msa.microscopy.com} ; Mon, 12 Mar 2007 10:12:17 -0400 (EDT) 5, 17 -- Message-ID: {45F55FBD.4050101-at-fccc.edu} 5, 17 -- Date: Mon, 12 Mar 2007 10:12:13 -0400 5, 17 -- From: Michal Jarnik {Michal.Jarnik-at-fccc.edu} 5, 17 -- User-Agent: Thunderbird 1.5.0.5 (Macintosh/20060719) 5, 17 -- MIME-Version: 1.0 5, 17 -- To: microscopy-at-msa.microscopy.com 5, 17 -- Subject: Nucleolar markers - antibodies for TEM 5, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The physical difference is the CS mount lens is designed to be mounted ~5mm closer to the image sensor than a C mount lens. (C-mount lenses are designed to be mounted 17.526mm in front of the image sensor vs. 12.5mm for CS-mount.) You can always use a C mount lens on a CS mount camera by using a 5mm spacer ring (many cameras now have C/CS selectable adjustment screws or rings). You can never use a CS mount lens on an older style C mount camera unless you are willing to physically modify the camera. Cost wise the CS mount lens is much less expensive since it uses fewer glass elements. Quality of image is the same. C mounts are becoming less and less popular and are generally only used on the more telephoto focal lengths such as 25, 50 and 75mm, and bigger zooms.
Both the C and CS mount are 1 inch wide (25.4mm) with 32 threads per inch (0.03125 inches or 0.79375mm). This dimension comes in handy if you need to insert a spacer to obtain proper focus. Unscrew the lens (or unscrew the camera from the mount in the case of telescope use and count the turns until proper focus is obtained. Multiply the above dimension by the number of turns to obtain the needed spacer or washer. (Washers are sometimes used as spacers if there are enough threads available.) Example: 1.25 turns x 0.79 mm = 0.9875 or ~1 mm. Many cameras (especially newer ones) have set screws to allow small adjustments in the distance between the lens and the image sensor.
AND
Definitions of CS-MOUNT on the Web:
* A relatively new industry standard for mounting a lens to a camera where a 1" X 32 thread is employed and the distance from the image plane from the shoulder of the lens is 12.52mm. A CS-mount lens may NOT be used on a C-mount camera. www.cbcamerica.com/cctvprod/glossary.htm
* "CS-mount" lenses have a flange back distance of 12.5mm vs. 17.526mm for "C-mount" lenses. Because of the shorter back focal distance, CS-mount lenses can only be used on CS-mount cameras. Your picture will be out of focus if you use a CS-mount lens on a C-mount camera. www.rainbowcctv.com/tech/lensterm.html
A threaded (screwmount) lens mount system developed by Tamron for use with older manual focus lenses and still commonly seen today on telescope to camera adapters (prime focus photography).
T-mounts are 42mm in diameter with a 0.75 mm thread pitch. They are thus not compatible with M42 mounts with their 1mm thread pitch, though they may look the same. You can cause damage to your equipment if you try to mate the wrong sized components. The "T" stands for Tamron and not telescope.
cf. astrophotography, M42, prime focus, thread pitch.
On 10 Mar 2007 at 22:16, donc-at-asmicro.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I browsed with interest the links mentioned recently concerning digital } camera adapters for photomicrography. On the vendor web pages, I see } mentioned T-mount, C-mount, CS-mount etc. Can anyone point me to a source } that defines the standard dimensions of various camera mounts such as these? } } regards, } Don Chernoff } ================================== } Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com } 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 } INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) } web: http://www.asmicro.com Fax: 317-895-5652 } [business activities: analytical services in AFM, AFM probes, consulting, } training, } calibration and test specimens, calibration and measurement software, } used NanoScope equipment.] } } } } ==============================Original Headers============================== } 4, 21 -- From donc-at-asmicro.com Sat Mar 10 22:15:16 2007 } 4, 21 -- Received: from smtp102.sbc.mail.mud.yahoo.com (smtp102.sbc.mail.mud.yahoo.com [68.142.198.201]) } 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2B4FGbO017284 } 4, 21 -- for {microscopy-at-microscopy.com} ; Sat, 10 Mar 2007 22:15:16 -0600 } 4, 21 -- Received: (qmail 65445 invoked from network); 11 Mar 2007 04:15:21 -0000 } 4, 21 -- Received: from unknown (HELO ASM11) (asmicro-at-sbcglobal.net-at-68.57.205.80 with login) } 4, 21 -- by smtp102.sbc.mail.mud.yahoo.com with SMTP; 11 Mar 2007 04:15:21 -0000 } 4, 21 -- X-YMail-OSG: 9QfmSEEVM1mbmoDSpk6B4Vk5UjNxazracQdSwvndGVowl5w3OHA9afZnedto1BgUyYj65NfW3wktWrFHZpCmmEFqcR1ycLl1Mll4GGdIADjodr6SFn0YjkplxDEIJYtbJMRS2tL_ntHE_a4- } 4, 21 -- Message-ID: {006301c76393$8e29e0c0$3301a8c0-at-ASM11} } 4, 21 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} } 4, 21 -- To: "Microscopy List" {microscopy-at-microscopy.com} } 4, 21 -- Subject: T, C, CS and other mounts } 4, 21 -- Date: Sat, 10 Mar 2007 23:11:24 -0500 } 4, 21 -- MIME-Version: 1.0 } 4, 21 -- Content-Type: text/plain; } 4, 21 -- charset="iso-8859-1" } 4, 21 -- Content-Transfer-Encoding: 7bit } 4, 21 -- X-Priority: 3 } 4, 21 -- X-MSMail-Priority: Normal } 4, 21 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1807 } 4, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1896 } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 25, 33 -- From edelmare-at-muohio.edu Mon Mar 12 09:51:47 2007 25, 33 -- Received: from spamfirewall.muohio.edu (walrus.mcs.muohio.edu [134.53.6.27]) 25, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2CEpl6e000835 25, 33 -- for {microscopy-at-Microscopy.com} ; Mon, 12 Mar 2007 09:51:47 -0500 25, 33 -- X-ASG-Debug-ID: 1173711103-40a2005d0000-Dem1zR 25, 33 -- X-Barracuda-URL: http://spamfirewall.muohio.edu:80/cgi-bin/mark.cgi 25, 33 -- X-Barracuda-Connect: mulnx23.mcs.muohio.edu[134.53.6.10] 25, 33 -- X-Barracuda-Start-Time: 1173711103 25, 33 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 25, 33 -- by spamfirewall.muohio.edu (Spam Firewall) with ESMTP 25, 33 -- id 8EF02A3951A; Mon, 12 Mar 2007 10:51:43 -0400 (EDT) 25, 33 -- Received: from [192.168.1.23] ([134.53.14.105]) 25, 33 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l2CEphoh024477; 25, 33 -- Mon, 12 Mar 2007 10:51:43 -0400 25, 33 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 25, 33 -- To: donc-at-asmicro.com 25, 33 -- Date: Mon, 12 Mar 2007 10:51:42 -0400 25, 33 -- MIME-Version: 1.0 25, 33 -- X-ASG-Orig-Subj: Re: [Microscopy] T, C, CS and other mounts 25, 33 -- Subject: Re: [Microscopy] T, C, CS and other mounts 25, 33 -- CC: microscopy-at-Microscopy.com 25, 33 -- Message-ID: {45F530BE.15067.5C3DCC-at-edelmare.muohio.edu} 25, 33 -- Priority: normal 25, 33 -- In-reply-to: {200703110416.l2B4GfDQ018549-at-ns.microscopy.com} 25, 33 -- References: {200703110416.l2B4GfDQ018549-at-ns.microscopy.com} 25, 33 -- X-mailer: Pegasus Mail for Windows (4.41) 25, 33 -- Content-type: text/plain; charset=ISO-8859-1 25, 33 -- Content-description: Mail message body 25, 33 -- X-Barracuda-Virus-Scanned: by Barracuda Spam Firewall at muohio.edu 25, 33 -- X-Barracuda-Spam-Score: 0.00 25, 33 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=3.5 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=1000.0 25, 33 -- Content-Transfer-Encoding: 8bit 25, 33 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id l2CEpl6e000835 ==============================End of - Headers==============================
I am new to LR White. The instructions for "Electron Microscopy" strongly recommends curing 20-24 hrs at 60 degrees plus or minus 2 degrees and warns of over brittle blocks if this is not followed. But the instructions for "Electron Microscopic Immunocytochemistry" recommends 50 degrees for 24 hours. My clients want LR White embedding for immunogold staining. I would appreciate feedback from regular users as to what temperature and curing time they use. Thanks.
Ralph Common Michigan State University Dept. of Physiology
==============================Original Headers============================== 4, 24 -- From rcommon-at-msu.edu Mon Mar 12 10:23:54 2007 4, 24 -- Received: from sys19.mail.msu.edu (sys19.mail.msu.edu [35.9.75.119]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2CFNsF8013069 4, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Mar 2007 10:23:54 -0500 4, 24 -- Received: from [35.9.122.125] (helo=emlab) 4, 24 -- by sys19.mail.msu.edu with esmtpsa (Exim 4.52 #1) 4, 24 -- (TLSv1:RC4-MD5:128) 4, 24 -- id 1HQmNO-0006Xv-50 4, 24 -- for Microscopy-at-microscopy.com; Mon, 12 Mar 2007 11:23:54 -0400 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: LR White polymerization 4, 24 -- Date: Mon, 12 Mar 2007 10:25:29 -0500 4, 24 -- Message-ID: {000201c764ba$ab504e50$7d7a0923-at-msu.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="iso-8859-1" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 (Normal) 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1896 4, 24 -- Importance: Normal 4, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
We got it to polymerise at 52-53 degrees for 24hrs. Worth trying 50 degrees with a blank block. We did not notice any sectioning problems.
dave
-----Original Message----- X-from: rcommon-at-msu.edu [mailto:rcommon-at-msu.edu] Sent: 12 March 2007 15:28 To: David Patton
I am new to LR White. The instructions for "Electron Microscopy" strongly recommends curing 20-24 hrs at 60 degrees plus or minus 2 degrees and warns of over brittle blocks if this is not followed. But the instructions for "Electron Microscopic Immunocytochemistry" recommends 50 degrees for 24 hours. My clients want LR White embedding for immunogold staining. I would appreciate feedback from regular users as to what temperature and curing time they use. Thanks.
Ralph Common Michigan State University Dept. of Physiology
==============================Original Headers============================== 4, 24 -- From rcommon-at-msu.edu Mon Mar 12 10:23:54 2007 4, 24 -- Received: from sys19.mail.msu.edu (sys19.mail.msu.edu [35.9.75.119]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2CFNsF8013069 4, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Mar 2007 10:23:54 -0500 4, 24 -- Received: from [35.9.122.125] (helo=emlab) 4, 24 -- by sys19.mail.msu.edu with esmtpsa (Exim 4.52 #1) 4, 24 -- (TLSv1:RC4-MD5:128) 4, 24 -- id 1HQmNO-0006Xv-50 4, 24 -- for Microscopy-at-microscopy.com; Mon, 12 Mar 2007 11:23:54 -0400 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: LR White polymerization 4, 24 -- Date: Mon, 12 Mar 2007 10:25:29 -0500 4, 24 -- Message-ID: {000201c764ba$ab504e50$7d7a0923-at-msu.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="iso-8859-1" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 (Normal) 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1896 4, 24 -- Importance: Normal 4, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
This incoming email to UWE has been independently scanned for viruses by McAfee anti-virus software and none were detected
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==============================Original Headers============================== 16, 34 -- From David.Patton-at-uwe.ac.uk Mon Mar 12 10:34:59 2007 16, 34 -- Received: from mailapp04.uwe.ac.uk (mailapp04.uwe.ac.uk [164.11.132.66]) 16, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2CFYweW024545 16, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Mar 2007 10:34:59 -0500 16, 34 -- Received: from (mta01.uwe.ac.uk [164.11.132.60]) by mailapp04.uwe.ac.uk with smtp 16, 34 -- id 64d3_81517678_d0ae_11db_8c49_00142223915c; 16, 34 -- Mon, 12 Mar 2007 15:30:02 +0000 16, 34 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 16, 34 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121]) 16, 34 -- by mta01.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 16, 34 -- 2005)) with ESMTP id {0JES0047WR9RI4-at-mta01.uwe.ac.uk} for 16, 34 -- Microscopy-at-microscopy.com; Mon, 12 Mar 2007 15:34:39 +0000 (GMT) 16, 34 -- Date: Mon, 12 Mar 2007 15:31:46 +0000 16, 34 -- From: David Patton {David.Patton-at-uwe.ac.uk} 16, 34 -- Subject: RE: [Microscopy] LR White polymerization 16, 34 -- In-reply-to: {200703121527.l2CFRgFG019963-at-ns.microscopy.com} 16, 34 -- To: rcommon-at-msu.edu 16, 34 -- Cc: Microscopy-at-microscopy.com 16, 34 -- Message-id: {F247F674896BE243AD8263C5280E2BDB02D90A32-at-egen-uwe01} 16, 34 -- MIME-version: 1.0 16, 34 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 16, 34 -- Content-type: text/plain; charset=us-ascii 16, 34 -- Content-class: urn:content-classes:message 16, 34 -- Thread-topic: [Microscopy] LR White polymerization 16, 34 -- Thread-index: AcdkuwKboce7DUaCSvyQVl+dawHyuQAADhpg 16, 34 -- X-MS-Has-Attach: 16, 34 -- X-MS-TNEF-Correlator: 16, 34 -- X-NAIMIME-Disclaimer: 1 16, 34 -- X-NAIMIME-Modified: 1 16, 34 -- X-NAI-Spam-Score: -1.2 16, 34 -- X-NAI-Spam-Rules: 1 Rules triggered 16, 34 -- BAYES_01=-1.2 16, 34 -- Content-Transfer-Encoding: 8bit 16, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2CFYweW024545 ==============================End of - Headers==============================
I polymerize LRW at 50 deg. C for immuno-gold labelling. It cuts nicely and has good beam stability. Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Weill-Cornell Medical College
This is a call out to all who might have spare specimens laying around.
We are a middle and high school with an SEM but no sample preparation equipment. So, until we can get some, I am asking for anyone who may have some spare samples that they are finished with (Biologicals especially this year! I would really like a specimen of a neuron of some variety to show the structure in 3D) or that are hanging around if they could send them our way so I can have a library of specimens to use in demonstrations. I can adapt any mount to our mount.
Please email me off list if you have something that you would be wiling to part with.
Thanks,
Justin A. Kraft Director Center for Inquiry-Based Science Education
==============================Original Headers============================== 5, 26 -- From kraftpiano-at-gmail.com Mon Mar 12 11:11:14 2007 5, 26 -- Received: from an-out-0708.google.com (an-out-0708.google.com [209.85.132.244]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2CGBEn6017097 5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 12 Mar 2007 11:11:14 -0500 5, 26 -- Received: by an-out-0708.google.com with SMTP id b20so1418101ana 5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 12 Mar 2007 09:11:13 -0700 (PDT) 5, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 5, 26 -- d=gmail.com; s=beta; 5, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 26 -- b=IkqS/zwR1tUYnP9Jv95mqECIt6gJdDaCw1pS92UR41M60zBqFAZ3Vf1TEX5bXTB6bLdYO7pWVzimolP9GIDb09IO9dRlkkUNAEWk5VLsTfak3g2j8OxqQfXtbZd8JaC+L1l5flaTm344egwoT8PFTSmMFUbB7ggblnIT8kl/n/c= 5, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 26 -- d=gmail.com; s=beta; 5, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 26 -- b=CV6j8l0Fkool+h1iel+apzF04QTUz2JDl9P3qaU/OUzUje39NW8E0TnWoDvTZZvl01eTA6SGVS8tZLyjr1rtlsJCoWkc0s9QQwwAhAlC0GLboLl5YrhZ9BQkwhTZ7MLb/rercMaz0UD4D49e9y//LxpZ6JRI138ELsNiGpJgrhA= 5, 26 -- Received: by 10.114.202.15 with SMTP id z15mr1791965waf.1173715873258; 5, 26 -- Mon, 12 Mar 2007 09:11:13 -0700 (PDT) 5, 26 -- Received: by 10.114.79.12 with HTTP; Mon, 12 Mar 2007 09:11:13 -0700 (PDT) 5, 26 -- Message-ID: {25e2b0d20703120911j348918eexd3eedecb5f0e5ddf-at-mail.gmail.com} 5, 26 -- Date: Mon, 12 Mar 2007 12:11:13 -0400 5, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 5, 26 -- To: microscopy-at-microscopy.com 5, 26 -- Subject: SEM Specimens. 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
Worthwhile bearing in mind the possibility of protein/antigen extraction during long infiltration periods in LR White and during slow polymerization at 50 degrees.
I (and others) have found that using LR White accelerator (1.5ul per ml) and immersing the molds in a crushed ice slush to be a better means of minimizing crosslinkage of the resin and thereby optimizing antibody access to the antigen.
A full description of this method and rational can be found in my immunogold review article in the Journal of Histotechnology/ vol 16, no 3/ Sept 1993.
Get back to me if you have difficulty in accessing this and want further details.
Regards,
Alastair Alastair McKinnon Histology & EM Facility Manager University of Aberdeen, Institute of Medical Science Foresterhill, Aberdeen, AB25 2ZD tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk www.abdn.ac.uk/ims/h-em -----Original Message----- X-from: rcommon-at-msu.edu [mailto:rcommon-at-msu.edu] Sent: 12 March 2007 15:28 To: Mckinnon, Alastair D.
I am new to LR White. The instructions for "Electron Microscopy" strongly recommends curing 20-24 hrs at 60 degrees plus or minus 2 degrees and warns of over brittle blocks if this is not followed. But the instructions for "Electron Microscopic Immunocytochemistry" recommends 50 degrees for 24 hours. My clients want LR White embedding for immunogold staining. I would appreciate feedback from regular users as to what temperature and curing time they use. Thanks.
Ralph Common Michigan State University Dept. of Physiology
==============================Original Headers============================== 4, 24 -- From rcommon-at-msu.edu Mon Mar 12 10:23:54 2007 4, 24 -- Received: from sys19.mail.msu.edu (sys19.mail.msu.edu [35.9.75.119]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2CFNsF8013069 4, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Mar 2007 10:23:54 -0500 4, 24 -- Received: from [35.9.122.125] (helo=emlab) 4, 24 -- by sys19.mail.msu.edu with esmtpsa (Exim 4.52 #1) 4, 24 -- (TLSv1:RC4-MD5:128) 4, 24 -- id 1HQmNO-0006Xv-50 4, 24 -- for Microscopy-at-microscopy.com; Mon, 12 Mar 2007 11:23:54 -0400 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: LR White polymerization 4, 24 -- Date: Mon, 12 Mar 2007 10:25:29 -0500 4, 24 -- Message-ID: {000201c764ba$ab504e50$7d7a0923-at-msu.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="iso-8859-1" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 (Normal) 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1896 4, 24 -- Importance: Normal 4, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
==============================Original Headers============================== 17, 24 -- From a.d.mckinnon-at-abdn.ac.uk Mon Mar 12 12:09:26 2007 17, 24 -- Received: from mailhub1.abdn.ac.uk (mailhub1.abdn.ac.uk [139.133.7.28]) 17, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2CH9Q6R030138 17, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Mar 2007 12:09:26 -0500 17, 24 -- Received: from ew-mail-a.uoa.abdn.ac.uk ([139.133.15.20] helo=VMAIL1.uoa.abdn.ac.uk) 17, 24 -- by mailhub1.abdn.ac.uk with esmtp (Exim 4.52) 17, 24 -- id 1HQo1V-0003hE-Ia; Mon, 12 Mar 2007 17:09:25 +0000 17, 24 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 17, 24 -- Content-class: urn:content-classes:message 17, 24 -- MIME-Version: 1.0 17, 24 -- Content-Type: text/plain; 17, 24 -- charset="us-ascii" 17, 24 -- Subject: RE: [Microscopy] LR White polymerization 17, 24 -- Date: Mon, 12 Mar 2007 17:08:54 -0000 17, 24 -- Message-ID: {7CA99BD491B8EF45BBF158CAEF7465336B100F-at-VMAIL1.uoa.abdn.ac.uk} 17, 24 -- X-MS-Has-Attach: 17, 24 -- X-MS-TNEF-Correlator: 17, 24 -- Thread-Topic: [Microscopy] LR White polymerization 17, 24 -- Thread-Index: Acdkuw7UxcPmA5FCTLKSSXX5Ss2v+AAB6c3w 17, 24 -- From: "Mckinnon, Alastair D." {a.d.mckinnon-at-abdn.ac.uk} 17, 24 -- To: {rcommon-at-msu.edu} 17, 24 -- Cc: {Microscopy-at-microscopy.com} 17, 24 -- Content-Transfer-Encoding: 8bit 17, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2CH9Q6R030138 ==============================End of - Headers==============================
Paper is one of my favorite specimens (the other two are insects and glass) for the initial demonstration of SEM capabilities to students. It always has a beautiful structure whether coated or non-coated (observed in low voltage or environmental mode), and it is very easy to handle. So, I do not understand why for you "grey scale differences were to low". If you can send me your images off-line we could discuss them in more detail.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} } Email: greta.rennings-at-web.de } Name: Greta Rennings } } Organization: KU Leuven } } Education: Graduate College } } Location: Leuven, Brabant, Belgium } } Question: Dear ladies and gentlemen, } } do you have an idea which microscopic technique would be suitable for } analysis of paper? } Firstly for 3D I did trials with CLSM, which were not truly successful
} as grey scale differences were too low. Other techniques I know would } require splitting or sectioning, as far as I know- do you have further
} experience? } Secondly for 2D I tried light microscopy and SEM, but grey scale } differences were to low here too in order to differentiate between } components. Could TEM be useful? What could help to get better } distinguishable features? } } Thank you very much in advance for your support! } Kind regards, } Greta } } -------------------------------------------------------------- } ------------- } } ==============================Original } Headers============================== } 9, 12 -- From zaluzec-at-ultra5.microscopy.com Wed Mar 7 } 16:40:39 2007 9, 12 -- Received: from [206.69.208.22] } (mac22.zaluzec.com [206.69.208.22]) } 9, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id l27MebWY022766 } 9, 12 -- for {microscopy-at-microscopy.com} ; Wed, 7 Mar } 2007 16:40:39 -0600 } 9, 12 -- Mime-Version: 1.0 } 9, 12 -- X-Sender: zaluzec-at-ultra5.microscopy.com (Unverified) 9, 12 --
} Message-Id: {p06110403c214efc7cdbb-at-[206.69.208.22]} } 9, 12 -- Date: Wed, 7 Mar 2007 16:40:36 -0600 9, 12 -- To: } microscopy-at-microscopy.com 9, 12 -- From: } greta.rennings-at-web.de (by way of Ask-A-Microscopist) 9, 12 -- } Subject: AskAMicroscopist: analysis of paper 9, 12 -- } Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers============================== }
==============================Original Headers============================== 9, 23 -- From DusevichV-at-umkc.edu Mon Mar 12 13:39:24 2007 9, 23 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2CIdOCu011491 9, 23 -- for {microscopy-at-msa.microscopy.com} ; Mon, 12 Mar 2007 13:39:24 -0500 9, 23 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.1830); 9, 23 -- Mon, 12 Mar 2007 13:39:22 -0500 9, 23 -- x-mimeole: Produced By Microsoft Exchange V6.5 9, 23 -- Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="us-ascii" 9, 23 -- Subject: Re: AskAMicroscopist: analysis of paper 9, 23 -- Date: Mon, 12 Mar 2007 13:39:22 -0500 9, 23 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB33ADC84-at-KC-MSX1.kc.umkc.edu} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: Re: AskAMicroscopist: analysis of paper 9, 23 -- Thread-Index: AcdhCbXz1hhH3w6QQKSIgGEH91N0IQDymeoAAABieNA= 9, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 9, 23 -- To: {microscopy-at-msa.microscopy.com} 9, 23 -- X-OriginalArrivalTime: 12 Mar 2007 18:39:22.0812 (UTC) FILETIME=[C0C24FC0:01C764D5] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2CIdOCu011491 ==============================End of - Headers==============================
Once again the list comes through with very valuable information. Thank you, everyone.
I do not have the answer yet, but as several folks asked when I come to some conclusions I will post back to the list.
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 5, 29 -- From edelmare-at-muohio.edu Mon Mar 12 15:03:53 2007 5, 29 -- Received: from spamfirewall.muohio.edu (wallaby.mcs.muohio.edu [134.53.6.28]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2CK3rc1024588 5, 29 -- for {microscopy-at-Microscopy.com} ; Mon, 12 Mar 2007 15:03:53 -0500 5, 29 -- X-ASG-Debug-ID: 1173729832-57e000100000-Dem1zR 5, 29 -- X-Barracuda-URL: http://spamfirewall.muohio.edu:80/cgi-bin/mark.cgi 5, 29 -- X-Barracuda-Connect: mulnx23.mcs.muohio.edu[134.53.6.10] 5, 29 -- X-Barracuda-Start-Time: 1173729832 5, 29 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 5, 29 -- by spamfirewall.muohio.edu (Spam Firewall) with ESMTP id 99BEE166FAE 5, 29 -- for {microscopy-at-Microscopy.com} ; Mon, 12 Mar 2007 16:03:52 -0400 (EDT) 5, 29 -- Received: from [192.168.1.23] ([134.53.14.105]) 5, 29 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l2CK3q2q027264 5, 29 -- for {microscopy-at-Microscopy.com} ; Mon, 12 Mar 2007 16:03:52 -0400 5, 29 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 5, 29 -- To: microscopy-at-Microscopy.com 5, 29 -- Date: Mon, 12 Mar 2007 16:03:52 -0400 5, 29 -- MIME-Version: 1.0 5, 29 -- X-ASG-Orig-Subj: Re: [Microscopy] EM Field sources 5, 29 -- Subject: Re: [Microscopy] EM Field sources 5, 29 -- Message-ID: {45F579E8.20526.17A04AA-at-edelmare.muohio.edu} 5, 29 -- Priority: normal 5, 29 -- X-mailer: Pegasus Mail for Windows (4.41) 5, 29 -- Content-type: text/plain; charset=US-ASCII 5, 29 -- Content-transfer-encoding: 7BIT 5, 29 -- Content-description: Mail message body 5, 29 -- X-Barracuda-Virus-Scanned: by Barracuda Spam Firewall at muohio.edu 5, 29 -- X-Barracuda-Spam-Score: 0.00 5, 29 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=3.5 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=1000.0 ==============================End of - Headers==============================
I have solicited similar requests and will pay for them.
The well is dry.
gary g.
At 08:12 AM 3/12/2007, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 7, 20 -- From gary-at-gaugler.com Tue Mar 13 00:50:44 2007 7, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2D5ohSF015285 7, 20 -- for {microscopy-at-microscopy.com} ; Tue, 13 Mar 2007 00:50:44 -0500 7, 20 -- Message-Id: {200703130550.l2D5ohSF015285-at-ns.microscopy.com} 7, 20 -- Received: (qmail 5488 invoked from network); 12 Mar 2007 22:49:47 -0800 7, 20 -- Received: by simscan 1.1.0 ppid: 5485, pid: 5486, t: 0.0889s 7, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 7, 20 -- by qsmtp4 with SMTP; 12 Mar 2007 22:49:47 -0800 7, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 20 -- Date: Mon, 12 Mar 2007 22:50:37 -0800 7, 20 -- To: kraftpiano-at-gmail.com 7, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 20 -- Subject: Re: [Microscopy] SEM Specimens. 7, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 7, 20 -- In-Reply-To: {200703121612.l2CGCshm020119-at-ns.microscopy.com} 7, 20 -- References: {200703121612.l2CGCshm020119-at-ns.microscopy.com} 7, 20 -- Mime-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-6C3B75C7 ==============================End of - Headers==============================
Sorry to reply so late but my private life took most of my time lately (no I was not in vacations ;-)). I just wanted to warmly thank all those who contacted me or replied to my query and apologize in the case I did not send a personal reply to everybody.
Stephane
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==============================Original Headers============================== 5, 20 -- From nizets2-at-yahoo.com Tue Mar 13 02:59:37 2007 5, 20 -- Received: from web37408.mail.mud.yahoo.com (web37408.mail.mud.yahoo.com [209.191.91.140]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2D7xadx028542 5, 20 -- for {microscopy-at-microscopy.com} ; Tue, 13 Mar 2007 02:59:37 -0500 5, 20 -- Received: (qmail 78051 invoked by uid 60001); 13 Mar 2007 07:59:36 -0000 5, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 20 -- s=s1024; d=yahoo.com; 5, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 5, 20 -- b=UYEdYTLCdScxGv9DDU3TPe7tAIpKXNWy/ecSkoJkSV8aybViwrHn841v3lA6A5k/h3fNZAAR2kuyXX3xodEdMNfRC7mLZdO2W+1f5JSR4/3IfcmkeWZq3t9FC/LVjwTTGUVKIS2A242DQj5iU8EOk14HZgHNA2+RyyvF4IG0ybg=; 5, 20 -- X-YMail-OSG: oooXQjcVM1kgbPQvuwTkYfl7Qw9UO4Jg_rY_QFuRGYAsWDvKBZyATDL0WBkEzmFbgMJacAu08UhlWy1M7aPqF5rUiBXu3RNQt0.u6Vgrqd.DS8t4MMw3FbaDVgZWL6QkrnVLK1dEFQADgzhhku2xIm3J 5, 20 -- Received: from [80.122.101.102] by web37408.mail.mud.yahoo.com via HTTP; Tue, 13 Mar 2007 00:59:36 PDT 5, 20 -- Date: Tue, 13 Mar 2007 00:59:36 -0700 (PDT) 5, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 20 -- Subject: Re: [Microscopy] mosquito by SEM - better late than never 5, 20 -- To: microscopy-at-microscopy.com 5, 20 -- In-Reply-To: {662078.15253.qm-at-web37411.mail.mud.yahoo.com} 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; charset=iso-8859-1 5, 20 -- Content-Transfer-Encoding: 8bit 5, 20 -- Message-ID: {524143.61873.qm-at-web37408.mail.mud.yahoo.com} ==============================End of - Headers==============================
MSORV (the Microscopy Society of the Ohio River Valley) is having their Spring Meeting on Wednesday, March 28, 2007 in Dayton, OH. The group has been in an "inactive status" for over 3 years and this will be their first meeting since the Fall of 2003. This first meeting is going to be shorter than normal (4 hours) beginning with a mixer/networking social hour, a Business meeting to discuss what direction and shape the society will take, and two talks. The Agenda is below. If you have questions or would like to be placed on our e-mail list to receive directions etc. please contact either myself or Dave Tomlin. Our e-mail addresses are: pamela.lloyd-at-wpafb.af.mil or david.tomlin-at-wpafb.af.mil.
Spring MSORV Meeting Location: UES, Inc. 4401 Dayton-Xenia Rd. Dayton, OH 45432
AGENDA *3-4 PM Opening Events:
Registration and Membership Applications Mixer (Sponsored by JEOL, Ltd.)
*4-5 PM Business Meeting:
MSORV: Role and Value of a Local Society Needs and Concerns of Members Format of Future Meetings Treasurer's Report: Dave Tomlin (UES / WPAFB) Chair: Matt Chestnut (Procter & Gamble)
*5-7 PM Scientific Presentations:
"WDS X-ray Analysis with Parallel Beam Spectrometers on Scanning Electron Microscopes" - Alan Sandborg (EDAX)
"Frontiers in Microscopy for Biological Specimens" - Wally Ip and Bob Hennigan (UC Medical School)
Pamela F. Lloyd Materials Engineer UES, Inc. AFRL/MLPJE Bldg. 651, Room 82 3005 Hobson Way WPAFB, OH 45433 Tel: 937-255-9413 Fax: 937-656-7292 e-mail: pamela.lloyd-at-wpafb.af.mil
==============================Original Headers============================== 14, 27 -- From Pamela.Lloyd-at-WPAFB.AF.MIL Tue Mar 13 06:05:33 2007 14, 27 -- Received: from fohfwb003.oh.afmc.af.mil (fohfwb003.oh.afmc.af.mil [131.28.29.206]) 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2DB5Xei011460 14, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Mar 2007 06:05:33 -0500 14, 27 -- Received: from fohmlrl04 (fohmlrl04.enterprise.afmc.ds.af.mil [131.28.34.158]) 14, 27 -- by fohfwb003.oh.afmc.af.mil with ESMTP id l2DB5WVD012573 14, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Mar 2007 07:05:32 -0400 (EDT) 14, 27 -- X-AuditID: 831c229e-000008d0000002d4-76-45f6857c179e 14, 27 -- Received: from VFOHMLAO13.Enterprise.afmc.ds.af.mil ([131.28.34.134]) by fohmlrl04 with Microsoft SMTPSVC(6.0.3790.1830); 14, 27 -- Tue, 13 Mar 2007 06:05:32 -0500 14, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 14, 27 -- Content-class: urn:content-classes:message 14, 27 -- MIME-Version: 1.0 14, 27 -- Content-Type: text/plain; 14, 27 -- charset="us-ascii" 14, 27 -- Subject: MSORV Spring Meeting 14, 27 -- Date: Tue, 13 Mar 2007 07:05:32 -0400 14, 27 -- Message-ID: {29A7C5F65D54744CBFD97CD62A33DEE90173DCA1-at-VFOHMLAO13.Enterprise.afmc.ds.af.mil} 14, 27 -- X-MS-Has-Attach: 14, 27 -- X-MS-TNEF-Correlator: 14, 27 -- Thread-Topic: MSORV Spring Meeting 14, 27 -- Thread-Index: AcdlX4SW0e+mqLk7RM2kQzDOzyXiSA== 14, 27 -- From: "Lloyd, Pamela F CTR USAF AFRL/MLPJE" {Pamela.Lloyd-at-WPAFB.AF.MIL} 14, 27 -- To: {Microscopy-at-microscopy.com} 14, 27 -- X-Brightmail-Tracker: AAAAAA== 14, 27 -- Content-Transfer-Encoding: 8bit 14, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2DB5Xei011460 ==============================End of - Headers==============================
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2DFEpud028868 for {MicroscopyListserverArchive-at-microscopy.com} ; Tue, 13 Mar 2007 10:14:51 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id l2DFEpgQ028866; Tue, 13 Mar 2007 10:14:51 -0500
Many thanks to everyone who replied, on or off-line, to my question about LR White polymerization. Most agreed that LR White polymerizes well at 50 degrees in 24 hours, and that sectioning and beam stability are good. There were, however, some interesting variations suggested. One respondent uses microwave polymerization. Another uses UV polymerization at 4 degrees. Another suggested that polymerization at 37 degrees for 3 days might reduce loss of antigenicity. Several people emphasized the importance of excluding oxygen and using gelatin capsules when using heat to polymerize, and one suggested degassing the resin prior to use.
The most interesting suggestions involved using the "cold cure" method. The instructions that come with the LR White kit recommend not using this method for immunogold because the exothermic reaction can heat the resin above 60 degrees. But Dr. McKinnon (J. Histotechnology 1993: 16(3)) and others report superior results with the cold method. Apparently, if the resin can be kept cold during curing, the low temperature and shorter curing time reduce loss of antigenicity.
A newer protocol using PTA during processing was also suggested. See Arch Histol Cytol 68 (5), 337-347 (2005).
Ralph Common Michigan State University Dept. of Physiology
==============================Original Headers============================== 5, 24 -- From rcommon-at-msu.edu Tue Mar 13 10:14:51 2007 5, 24 -- Received: from sys29.mail.msu.edu (sys29.mail.msu.edu [35.9.75.129]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2DFEpSp028863 5, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Mar 2007 10:14:51 -0500 5, 24 -- Received: from [35.9.122.125] (helo=emlab) 5, 24 -- by sys29.mail.msu.edu with esmtpsa (Exim 4.52 #1) 5, 24 -- (TLSv1:RC4-MD5:128) 5, 24 -- id 1HR8iB-0002Ia-6P 5, 24 -- for Microscopy-at-microscopy.com; Tue, 13 Mar 2007 11:14:51 -0400 5, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 5, 24 -- To: {Microscopy-at-microscopy.com} 5, 24 -- Subject: LR White polymerization 5, 24 -- Date: Tue, 13 Mar 2007 10:16:26 -0500 5, 24 -- Message-ID: {003501c76582$9212d4d0$7d7a0923-at-msu.edu} 5, 24 -- MIME-Version: 1.0 5, 24 -- Content-Type: text/plain; 5, 24 -- charset="iso-8859-1" 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- X-Priority: 3 (Normal) 5, 24 -- X-MSMail-Priority: Normal 5, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 5, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1896 5, 24 -- Importance: Normal 5, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
} -----Original Message----- } From: Peter Tomic [mailto:peter.tomic-at-renwireless.com] } Sent: Monday, March 12, 2007 1:57 PM } To: Dusevich, Vladimir } Subject: Re: [Microscopy] Re: AskAMicroscopist: analysis of paper } } Vladimir, } } Just out of curiosity, what do you find interesting about glass? } Fracture surfaces? } } Best regards, } } Peter Tomic
For my first demonstration of SEM for students I start usually with insects. They are supermodels, divas of SEM imaging, they unfailingly get students excited and prepare them to consume information. Then comes paper. Nice images of fibers, difference in morphology of writing paper and filter paper, analysis of filler particles with BSE and EDS. Then I use three pieces of glass (coverslips): one clean (coated), one "washed" with tap water and air dried (coated) and one not coated.
On clean glass I start with focusing on dust particle, then move to a place without any particles, and students are somewhat surprised to see that there are nothing to look at: just dull monitor with the same brightness all over. So, I have to remind them, that when we look at glass with our eyes we do not see any features of its surface, but just light reflections. It is a good starting point for a brief discussion of a probe (whether electron beam or light) interaction with specimen and dependence of a resulting signal on topography.
On "washed" glass students can see a lot of crystals of salts. It leads to discussion of artifacts in microscopy and importance of proper specimen preparation.
Not coated glass, of course, is good for demonstration of charging. Different levels of charging - at 15 kV, when all we see are artifacts, and at 500 V, when we can get pretty decent image of glass surface (with dust particles). Finally I demonstrate the extreme case of charging. I switch off beam, move to a not charged (not previously observed) place on glass, set high voltage at 15-25 kV and scanning to a spot mode, turn beam on and charge glass for a few seconds. Then I set voltage to 2-5 kV and get image of a specimen chamber. Really nice "fish eye" view of a specimen chamber with an eye in a place of a specimen. I can change magnification, move image, focusing on some details, such as detectors, wires, etc. Students, seeing that with simple manipulations we converted our specimen in a device for observation of specimen chamber, get excited again, almost as much as when seeing insects. And excitement, I believe, really helps to remember lesson.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} DusevichV-at-umkc.edu wrote: } } } ---------------------------------------------------------------------- } } ------ The Microscopy ListServer -- CoSponsor: The } Microscopy Society } } of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------- } } ------ } } } } Paper is one of my favorite specimens (the other two are insects and } } glass) for the initial demonstration of SEM capabilities to } students. } } It always has a beautiful structure whether coated or non-coated } } (observed in low voltage or environmental mode), and it is } very easy to handle. } } So, I do not understand why for you "grey scale differences were to } } low". If you can send me your images off-line we could } discuss them in } } more detail. } } } } Vladimir } } } } Vladimir M. Dusevich, Ph.D. } } Electron Microscope Lab Manager } } 371 School of Dentistry } } 650 E. 25th Street } } Kansas City, MO 64108-2784 } } } } Phone: (816) 235-2072 } } Fax: (816) 235-5524 } } Web: http://www.umkc.edu/dentistry/microscopy } } } } } } } Email: greta.rennings-at-web.de } } } Name: Greta Rennings } } } } } } Organization: KU Leuven } } } } } } Education: Graduate College } } } } } } Location: Leuven, Brabant, Belgium } } } } } } Question: Dear ladies and gentlemen, } } } } } } do you have an idea which microscopic technique would be } suitable for } } } analysis of paper? } } } Firstly for 3D I did trials with CLSM, which were not truly } } } successful } } } } } as grey scale differences were too low. Other techniques I } know would } } } require splitting or sectioning, as far as I know- do you have } } } further } } } } } experience? } } } Secondly for 2D I tried light microscopy and SEM, but grey scale } } } differences were to low here too in order to differentiate between } } } components. Could TEM be useful? What could help to get better } } } distinguishable features? } } } } } } Thank you very much in advance for your support! } } } Kind regards, } } } Greta } } } } } } -------------------------------------------------------------- } } } ------------- } } } } } } ==============================Original } } } Headers============================== } } } 9, 12 -- From zaluzec-at-ultra5.microscopy.com Wed Mar 7 } } } 16:40:39 2007 9, 12 -- Received: from [206.69.208.22] } } } (mac22.zaluzec.com [206.69.208.22]) } } } 9, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } } } with ESMTP id l27MebWY022766 } } } 9, 12 -- for {microscopy-at-microscopy.com} ; Wed, 7 Mar } } } 2007 16:40:39 -0600 } } } 9, 12 -- Mime-Version: 1.0 } } } 9, 12 -- X-Sender: zaluzec-at-ultra5.microscopy.com } (Unverified) 9, 12 } } } -- } } } } } Message-Id: {p06110403c214efc7cdbb-at-[206.69.208.22]} } } } 9, 12 -- Date: Wed, 7 Mar 2007 16:40:36 -0600 9, 12 -- To: } } } microscopy-at-microscopy.com 9, 12 -- From: } } } greta.rennings-at-web.de (by way of Ask-A-Microscopist) 9, 12 -- } } } Subject: AskAMicroscopist: analysis of paper 9, 12 -- } } } Content-Type: text/plain; charset="us-ascii" } } } ==============================End of - } } } Headers============================== } } } } } } } } } ==============================Original } } Headers============================== } } 9, 23 -- From DusevichV-at-umkc.edu Mon Mar 12 13:39:24 2007 9, 23 -- } } Received: from kc-msxproto3.kc.umkc.edu } (kc-msxproto3.kc.umkc.edu [134.193.44.10]) } } 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id l2CIdOCu011491 } } 9, 23 -- for {microscopy-at-msa.microscopy.com} ; Mon, 12 } Mar 2007 13:39:24 -0500 } } 9, 23 -- Received: from KC-MSX1.kc.umkc.edu } ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft } SMTPSVC(6.0.3790.1830); } } 9, 23 -- Mon, 12 Mar 2007 13:39:22 -0500 } } 9, 23 -- x-mimeole: Produced By Microsoft Exchange V6.5 9, 23 -- } } Content-class: urn:content-classes:message 9, 23 -- } MIME-Version: 1.0 } } 9, 23 -- Content-Type: text/plain; } } 9, 23 -- charset="us-ascii" } } 9, 23 -- Subject: Re: AskAMicroscopist: analysis of paper 9, 23 -- } } Date: Mon, 12 Mar 2007 13:39:22 -0500 9, 23 -- Message-ID: } } {032EC4F75A527A4FA58C5B1B5DECFBB33ADC84-at-KC-MSX1.kc.umkc.edu} } } 9, 23 -- X-MS-Has-Attach: } } 9, 23 -- X-MS-TNEF-Correlator: } } 9, 23 -- Thread-Topic: Re: AskAMicroscopist: analysis of } paper 9, 23 } } -- Thread-Index: AcdhCbXz1hhH3w6QQKSIgGEH91N0IQDymeoAAABieNA= } } 9, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 9, } 23 -- To: } } {microscopy-at-msa.microscopy.com} 9, 23 -- } X-OriginalArrivalTime: 12 Mar } } 2007 18:39:22.0812 (UTC) FILETIME=[C0C24FC0:01C764D5] 9, 23 -- } } Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from } } quoted-printable to 8bit by ns.microscopy.com id l2CIdOCu011491 } } ==============================End of - } } Headers============================== } } -- } This email message is for the sole use of the intended } recipient(s) and may contain confidential and privileged } information. 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==============================Original Headers============================== 12, 25 -- From DusevichV-at-umkc.edu Tue Mar 13 10:45:45 2007 12, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (kc-msxproto2.kc.umkc.edu [134.193.143.155]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2DFjg9s008935 12, 25 -- for {microscopy-at-msa.microscopy.com} ; Tue, 13 Mar 2007 10:45:43 -0500 12, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.1830); 12, 25 -- Tue, 13 Mar 2007 10:45:40 -0500 12, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5 12, 25 -- Content-class: urn:content-classes:message 12, 25 -- MIME-Version: 1.0 12, 25 -- Content-Type: text/plain; 12, 25 -- charset="us-ascii" 12, 25 -- Subject: RE: [Microscopy] Re: AskAMicroscopist: analysis of paper 12, 25 -- Date: Tue, 13 Mar 2007 10:45:40 -0500 12, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB33ADC85-at-KC-MSX1.kc.umkc.edu} 12, 25 -- In-Reply-To: {45F5A26C.2040405-at-renwireless.com} 12, 25 -- X-MS-Has-Attach: 12, 25 -- X-MS-TNEF-Correlator: 12, 25 -- Thread-Topic: [Microscopy] Re: AskAMicroscopist: analysis of paper 12, 25 -- Thread-Index: Acdk2CB9JGzXhAH+TlyW5ulgMm8sCAArgOkg 12, 25 -- References: {200703121848.l2CIm2Rs022011-at-ns.microscopy.com} {45F5A26C.2040405-at-renwireless.com} 12, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 12, 25 -- To: {microscopy-at-msa.microscopy.com} 12, 25 -- X-OriginalArrivalTime: 13 Mar 2007 15:45:40.0989 (UTC) FILETIME=[A74812D0:01C76586] 12, 25 -- Content-Transfer-Encoding: 8bit 12, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2DFjg9s008935 ==============================End of - Headers==============================
There are several U.S. manufacturers of floor model (full scale with diffusion pump) vacuum evaporators and I'd be surprised if they cost anywhere close to 55K.
The Ladd digital vacuum system, complete with mechanical and diffusion pumps built in sells for less than 20K. In fact you could add a turbo and quartz thickness monitor and still be only about 30K.
In the 1960's when we started building our original vacuum systems the price was about 6K so they really haven't increased that much in 40 years.
Some evaporation (Brand X) units are quite expensive. I remember pricing a Brand X evaporator several years ago and nearly passed out when the quotation came back (over $30K). We decided to keep our nearly 40 yr old (yet still serviceable) Brand X unit that was purring alongside our 35 yr old Ladd unit (that cost around $8K). (We needed two units since one was used for ultraclean evaporative work and the other by trainees.)
As an aside: several months after I took my first job in Philadelphia (in 1976), an enormous wooden box arrived in my laboratory. After unpacking it, I discovered a Ladd evaporator that Margaret Ladd had kindly sent for me to "try out" for several months (no strings attached). After 6 months, I passed it along to another investigator. That certainly made a lasting impression on me. To this day, I have no idea how she found out that I was a microscopist working at The Medical College of Pennsylvania. I've never had such an offer since then.
-- ############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Email: bozzola-at-siu.edu ##############################################################
==============================Original Headers============================== 4, 18 -- From bozzola-at-siu.edu Tue Mar 13 16:14:48 2007 4, 18 -- Received: from abbmta2.siu.edu (abbmta2.siu.edu [131.230.254.206]) 4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2DLEmaZ005806 4, 18 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 13 Mar 2007 16:14:48 -0500 4, 18 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) 4, 18 -- by abbmta2.siu.edu (Switch-3.1.11/Switch-3.1.11) with ESMTP id l2DLElRA026510 4, 18 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 13 Mar 2007 16:14:48 -0500 (CDT) 4, 18 -- Mime-Version: 1.0 4, 18 -- X-Sender: bozzola-at-saluki-mail.siu.edu 4, 18 -- Message-Id: {p06110400c21cbece6752-at-[131.230.177.142]} 4, 18 -- Date: Tue, 13 Mar 2007 16:14:46 -0500 4, 18 -- To: Microscopy-at-msa.microscopy.com 4, 18 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 4, 18 -- Subject: Re: Carbon Evaporation Coaters 4, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 18 -- X-Spam-Score: 0.00% 4, 18 -- X-MASF: 0.00% 4, 18 -- X-Whitelist: 0.00% ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Jerry.L.Lehman-at-NXP.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Jerry.L.Lehman-at-NXP.com Name: Jerry L. Lehman
Organization: NXP Semiconductors
Title-Subject: [Filtered] Position Available: Failure Analysis Engineer
Question: NXP Semiconductors (Formerly Philips Semiconductors) is expanding its FA Department and is advertising the following position (Job # 2088) at Fishkill, NY, USA:
Job Responsibilities: ï Physical and electrical failure analyses and reporting of analogue and digital circuit IC¥s ï Support of design, yield and reliability improvements for products and processes ï Support of development projects ï Support of quality initiatives including automotive/zero defect ï Procedurization and continuous improvement of analysis methods
Experience Profile: Bachelor/ Master Degree in Electrical Engineering, Materials Science, Communication Engineering, Electronics or Physics ï Detailed knowledge in semiconductor physics and technology, analogue and digital signal processing and communication engineering ï 1-2yrs internship or other experience in Failure Analysis ï Some knowledge of TEM and/or FIB is a plus ï Fluent English conversation and writing ï Good knowledge in standard office tools ï Excellent analytical reasoning, communication skills, and organizational skills ï Team oriented, customer-centric quality mindset
If interested, please submit your resume thru the following link: http://www.nxp.com/jobs/search/index.html and search by JOB ID # 2088
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both marissagowrie-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: University of the West Indies St. Augustine
Title-Subject: [Filtered] Preparing Pollen Grains for Electron Microscopy
Question: Hello, I am a post graduate student at the University presently studying the transport of pollen grains in dust. I have been using the Burkard 7 day Spore Sampler to trap the pollen grains and stain them for viewing under the light microscope. I am presently exploring taking electron micrographs of the pollen grains but this would require removing the grains from the greased melinex tape of the sampler and mounting it onto the swab. I have come across the acetolysis process but this does not involve the removal of the pollen grain off the greased melinex tape. Does anyone know of a technique that can be used to remove the pollen from the tape (by dissolving the tape perphaps?) so that they can be placed on the swab? I look forward to your feedback. Thanks Marissa
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both AMCGroup2-at-aol.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: AMCGroup2-at-aol.com Name: Jim Glossinger
Organization: AMC Group
Title-Subject: [Filtered] TEM image cataloging-archiveing software
Question: Dear all,
We are currently evaluating multi-user, multi-location access Linux-based software products for cataloging-archiving our TEM image database.
Your input is greatly appreciated.
Regards,
Jim Glossinger, Ph.D. Principal Scientist AMC Group
First of all, thank you to everyone who has replied with offers of specimens! You guys are fantastic!
Now the fun bit:
I was sorting through a closet, and I found the Denton Desk II Carbon Accessory I have (I believe I offered it up in a previous post as trade for something useful...)
Anyway, I was wondering if there were a way to use this to do some carbon coating without the Denton Desk II Base unit. It's got the power supply, and I can fashion a chamber of some sort over the business end, but can it be done? I have an ample supply of carbon rods that came with it.
--Justin A. Kraft Director Center for Inquiry-Based Science Education
==============================Original Headers============================== 5, 26 -- From kraftpiano-at-gmail.com Tue Mar 13 21:41:43 2007 5, 26 -- Received: from nf-out-0910.google.com (nf-out-0910.google.com [64.233.182.191]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2E2fhrF024063 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 13 Mar 2007 21:41:43 -0500 5, 26 -- Received: by nf-out-0910.google.com with SMTP id a4so25176nfc 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 13 Mar 2007 19:41:42 -0700 (PDT) 5, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 5, 26 -- d=gmail.com; s=beta; 5, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 26 -- b=jcdp+8oRNQbbVhO4XkRXzVFmDomQ5nYmnpOnnfGs5ebnuGputXTsMvH4Jk6ZaiGkLv+l7LIAV1zB+jxXV0Y9UZh101vwd/JlHsDxWPb3yYVQ9b7n/s9/fWPQwaT6Pc3tuOXaMuUUlzccnVHRol9oYixstW6Vip0a6BfIt+TeWIU= 5, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 26 -- d=gmail.com; s=beta; 5, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 26 -- b=W5dq9XJauWnREIZ1juJnpVYutThrOJeHZ5/7vPYK6k/pyeDy3+702nL+z//NOBuGyeaEK6jo7aOL3bz5E/UedlFMQcdiG75jnTpEYXDM6jGU9j9ib3p0iYIyjXX5aspn8SpTWfCJoAfAwQ4ejo/M1UENwO9lTsGn++2Im9kBQw0= 5, 26 -- Received: by 10.114.60.19 with SMTP id i19mr2726745waa.1173840101648; 5, 26 -- Tue, 13 Mar 2007 19:41:41 -0700 (PDT) 5, 26 -- Received: by 10.114.79.12 with HTTP; Tue, 13 Mar 2007 19:41:41 -0700 (PDT) 5, 26 -- Message-ID: {25e2b0d20703131941t70909692tfac06d96b790808d-at-mail.gmail.com} 5, 26 -- Date: Tue, 13 Mar 2007 22:41:41 -0400 5, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 5, 26 -- To: microscopy-at-microscopy.com 5, 26 -- Subject: SEM: Coater home-brew? 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
I posted for specimens for payment and got no responses.
Should I have posted for them as free? I seek bio specimens (bacteria, parasites) and I will pay for them. I do not have CPD and HMDS, etc. capability. If free is better and more successful than paid, let me know.
gary g.
At 06:43 PM 3/13/2007, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Tue Mar 13 22:46:55 2007 11, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2E3ktHv004665 11, 20 -- for {microscopy-at-microscopy.com} ; Tue, 13 Mar 2007 22:46:55 -0500 11, 20 -- Message-Id: {200703140346.l2E3ktHv004665-at-ns.microscopy.com} 11, 20 -- Received: (qmail 14738 invoked from network); 13 Mar 2007 20:46:54 -0700 11, 20 -- Received: by simscan 1.1.0 ppid: 14735, pid: 14736, t: 0.0999s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 20 -- by qsmtp4 with SMTP; 13 Mar 2007 20:46:54 -0700 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Tue, 13 Mar 2007 20:46:49 -0800 11, 20 -- To: kraftpiano-at-gmail.com 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] SEM: Coater home-brew? 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200703140243.l2E2hcBU026979-at-ns.microscopy.com} 11, 20 -- References: {200703140243.l2E2hcBU026979-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-4D996365 ==============================End of - Headers==============================
This may be a simple question for those who are working on protein observed by TEM. What is the best supporting film for protein TEM work, silicon monoxide grids or ultra thin carbon film grids? If you can tell me the reason and the products your are using, it will be very helpful. Thanks, Jeffery
==============================Original Headers============================== 3, 20 -- From jzheng-at-uci.edu Tue Mar 13 23:09:08 2007 3, 20 -- Received: from relay2.es.uci.edu (relay2.es.uci.edu [128.200.80.28]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2E498Jo016244 3, 20 -- for {microscopy-at-microscopy.com} ; Tue, 13 Mar 2007 23:09:08 -0500 3, 20 -- Received: from webmail.uci.edu (webmail4.es.uci.edu [128.200.80.39]) 3, 20 -- by relay2.es.uci.edu (8.13.1/8.13.1) with ESMTP id l2E49700027854 3, 20 -- for {microscopy-at-microscopy.com} ; Tue, 13 Mar 2007 21:09:07 -0700 3, 20 -- Received: from 128.195.177.193 3, 20 -- (SquirrelMail authenticated user jzheng) 3, 20 -- by webmail.uci.edu with HTTP; 3, 20 -- Tue, 13 Mar 2007 21:09:07 -0700 (PDT) 3, 20 -- Message-ID: {4245.128.195.177.193.1173845347.squirrel-at-webmail.uci.edu} 3, 20 -- Date: Tue, 13 Mar 2007 21:09:07 -0700 (PDT) 3, 20 -- Subject: best supporting film for protein TEM 3, 20 -- From: jzheng-at-uci.edu 3, 20 -- To: microscopy-at-microscopy.com 3, 20 -- User-Agent: SquirrelMail/1.4.9a 3, 20 -- MIME-Version: 1.0 3, 20 -- Content-Type: text/plain;charset=iso-8859-1 3, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Emory University School of Medicine Microscopy Core is hosting a Cryo Technique and Immunogold labeling hands on workshop from Aug. 12 through Aug. 16. Here is some logistical information. Please contact Hong Yi at hyi-at-emory.edu or (404) 712-8491 for more information and registration procedure.
1. Date and Curriculum
Aug. 12-13: · Cryo-ultramicrotomy · A new cryo-fixation method · Set up for cryo-substitution Aug. 14-16: · The properties of gold particles and their protein conjugates. · Theories underlying immunogold labeling protocols. · Silver enhancement of gold particles · Imunogold labeling on a variety of sample preparations for LM. · Immunogold labeling for EM a. Pre-embedding immunogold labeling using ultrasmall gold conjugates and silver enhancement. b. Post-embedding immunogold labeling using conventional colloidal gold conjugates and ultrasmall gold conjugates.
Numerous biological microscopy techniques will be also demonstrated during the workshop. Details TBA
2. Main Instructors and Sponsors
Dr. Jan Leunissen, Aurion Mr. Helmut Gnägi, Diatome Hong Yi, Emory University
Leica Microsystems Aurion ImmunoGold Reagents Electron Microscopy Sciences/Diatome Hitachi High Technologies America, Inc
3. Fees
Session A: Cryo-technique: $500 Session B: Immunogold: $500 Session A and B: $800
Participants can sign up for either the entire workshop or a particular session of the workshop. If desired, participants who sign up for cryo-techniques will have additional practice time after lectures and training during the first two days. Applicants signing up for both sessions will be given first priority for enrollment.
4. Participants
The enrollment is open to anyone with interest to learn regardless of previous experience. However, due to limited space availability, the number of participants will be limited to 12 for the cryo-techniques and 20 for immunogold labeling.
5. Lodging
Participants are responsible for making hotel reservation themselves. The workshop will block a number of rooms at the following hotels
Villa International: (404) 633-6783, $24/night/person (double occupancy), or $36/night/person (single occupancy)
This hotel is cozy and clean and often used by Emory to house temporary or visiting employees. However, TV and phone are only available in the hotel common room.
Emory Inn: (800) 933-6679, $107/night or higher
Hong Yi Emory SOM EM
==============================Original Headers============================== 20, 17 -- From hyi-at-emory.edu Wed Mar 14 00:17:30 2007 20, 17 -- Received: from rwcrmhc12.comcast.net (rwcrmhc12.comcast.net [204.127.192.82]) 20, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2E5HUuS028547 20, 17 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2007 00:17:30 -0500 20, 17 -- Received: from [76.17.86.213] (c-76-17-86-213.hsd1.ga.comcast.net[76.17.86.213]) 20, 17 -- by comcast.net (rwcrmhc12) with SMTP 20, 17 -- id {20070314051729m1200ik7b1e} ; Wed, 14 Mar 2007 05:17:29 +0000 20, 17 -- Mime-Version: 1.0 (Apple Message framework v752.2) 20, 17 -- Message-Id: {3B613301-3BB5-4110-949A-D912AFA996E6-at-emory.edu} 20, 17 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 20, 17 -- To: Microscopy-at-microscopy.com 20, 17 -- From: Hong Yi {hyi-at-emory.edu} 20, 17 -- Subject: Workshop Announcement 20, 17 -- Date: Wed, 14 Mar 2007 01:17:27 -0400 20, 17 -- X-Mailer: Apple Mail (2.752.2) 20, 17 -- Content-Transfer-Encoding: 8bit 20, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2E5HUuS028547 ==============================End of - Headers==============================
Hi Jeffery, } What is the best supporting film for protein TEM work, } silicon monoxide grids or ultra thin carbon film grids? If you can } tell me } the reason and the products your are using, it will be very helpful.
in our experience, the best film is a home-made carbon-film (evaporated onto freshly cleaved mica sheets; by resistence evaporation or even better by electron gun evaporation), and putting them onto 400 or 600 mesh copper grids. Before applying the sample: glow-discharge the grid.
We have no experience with silicon monoxide grids.
best regards, Reinhard Rachel
---------------------- PD Dr. Reinhard Rachel Universitaet Regensburg Centre for EM - NWF III - Lehrstuhl fuer Anatomie Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720, 1666(TEM) fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-r.de office: VKL 3.1.29
==============================Original Headers============================== 7, 26 -- From reinhard.rachel-at-biologie.uni-regensburg.de Wed Mar 14 04:59:54 2007 7, 26 -- Received: from rrzmta1.rz.uni-regensburg.de (rrzmta1.rz.uni-regensburg.de [194.94.155.51]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2E9xsbl012286 7, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2007 04:59:54 -0500 7, 26 -- Received: from rrzmta1.rz.uni-regensburg.de (localhost [127.0.0.1]) 7, 26 -- by localhost (Postfix) with SMTP id 4A2611E7A95; 7, 26 -- Wed, 14 Mar 2007 10:59:58 +0100 (CET) 7, 26 -- Received: from localhost (donald1.rz.uni-regensburg.de [132.199.4.91]) 7, 26 -- by rrzmta1.rz.uni-regensburg.de (Postfix) with ESMTP id 38EA61E950C; 7, 26 -- Wed, 14 Mar 2007 10:59:58 +0100 (CET) 7, 26 -- Received: from dhcp8003.rz.uni-regensburg.de (dhcp8003.rz.uni-regensburg.de [132.199.87.3]) 7, 26 -- by webmail.uni-regensburg.de (IMP) with HTTP 7, 26 -- for {rar04520-at-rrzlic2.uni-regensburg.de} ; Wed, 14 Mar 2007 10:59:53 +0100 7, 26 -- Message-ID: {1173866393.45f7c79969bf4-at-webmail.uni-regensburg.de} 7, 26 -- Date: Wed, 14 Mar 2007 10:59:53 +0100 7, 26 -- From: reinhard rachel {reinhard.rachel-at-biologie.uni-regensburg.de} 7, 26 -- To: jzheng-at-uci.edu 7, 26 -- Cc: Microscopy-at-microscopy.com 7, 26 -- Subject: Re: [Microscopy] best supporting film for protein TEM 7, 26 -- References: {200703140409.l2E49Mvo016721-at-ns.microscopy.com} 7, 26 -- In-Reply-To: {200703140409.l2E49Mvo016721-at-ns.microscopy.com} 7, 26 -- MIME-Version: 1.0 7, 26 -- Content-Type: text/plain; charset=ISO-8859-1 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 7, 26 -- X-Originating-IP: 132.199.87.3 ==============================End of - Headers==============================
Hi Marissa, I have to admit I'm a little surprised. When I was more active with pollen collecting, I found Ronald Kapp's acetolysis method to dissolve/attack just about everything accept the pollen exine wall! The problem, is after acetolysis you can only compare those grains to reference grains which have been prepared the same way. When I examine air dried pollen from dust, I see that in many cases it's not a very good match to my acetolysis collection or to the key in Kapp's book. Still, it's better than nothing.
Not being familiar with the system you are using I can't comment on solvents, but I would try solvent, followed by dehydrating agents to remove any water condensed from the evaporative cooling of the solvents and compare those samples to air dried reference samples.
Have fun and let us know how it turns out for you.
stay safe............Frank
marissagowrie-at-yah oo.com To: frank.karl-at-degussa.com cc: 03/13/2007 07:52 Subject: [Microscopy] viaWWW: Preparing Pollen Grains for Electron Microscopy PM Please respond to marissagowrie
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Organization: University of the West Indies St. Augustine
Title-Subject: [Filtered] Preparing Pollen Grains for Electron Microscopy
Question: Hello, I am a post graduate student at the University presently studying the transport of pollen grains in dust. I have been using the Burkard 7 day Spore Sampler to trap the pollen grains and stain them for viewing under the light microscope. I am presently exploring taking electron micrographs of the pollen grains but this would require removing the grains from the greased melinex tape of the sampler and mounting it onto the swab. I have come across the acetolysis process but this does not involve the removal of the pollen grain off the greased melinex tape. Does anyone know of a technique that can be used to remove the pollen from the tape (by dissolving the tape perphaps?) so that they can be placed on the swab? I look forward to your feedback. Thanks Marissa
Jeffrey Zheng wrote: ===================================================== This may be a simple question for those who are working on protein observed by TEM. What is the best supporting film for protein TEM work, silicon monoxide grids or ultra thin carbon film grids? If you can tell me the reason and the products your are using, it will be very helpful. ================================================== There is a third option, that being the use of silicon nitride membrane window grids, see URL http://www.2spi.com/catalog/instruments/silicon-nitride-membrane-window-grids-slot-square.html If you are not familiar with these grids, the above website page should answer your questions.
The 20 or 30 nm thick membrane grids are used for this kind of work. At that thickness, they are very electron transparent and have the added advantage (over either silicon monoxide/silicon dioxide or ultra thin or any other carbon filmed grids) of not having any grain structure to interfere with the visualization of your protein samples.
Another advantage of the silicon nitride membrane window grids is that there is no "grid sag" as would normally occur with any "filmed" grid, be it silicon monoxide/silicon dioxide or carbon. With "sag", one never knows for sure what really is the magnification, or putting it another way, the sag introduces an error in any ultimate magnification calculation. The membrane window grids however have outstanding flatness and therefore (in comparison) no "sag" and this error in magnification measurement can be eliminated.
Finally, the membrane window grids are inherently more stable in the electron beam and one can expect lower numbers for image drift under exposure to the electron beam.
Disclaimer: SPI Supplies offers a full range of silicon nitride and silicon oxide membrane window grids so we would have a vested interest in seeing more people using them. The information given above is somewhat of a composite of what we have been told by present customers.
Chuck ================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
==============================Original Headers============================== 9, 25 -- From cgarber-at-2spi.com Wed Mar 14 08:49:48 2007 9, 25 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2EDnlpP007326 9, 25 -- for {microscopy-at-msa.microscopy.com} ; Wed, 14 Mar 2007 08:49:47 -0500 9, 25 -- Received: from webmail.idv.net (diskless6.external [209.120.196.50]) 9, 25 -- (authenticated bits=0) 9, 25 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id l2EDnlCp019684 9, 25 -- for {microscopy-at-msa.microscopy.com} ; Wed, 14 Mar 2007 09:49:47 -0400 9, 25 -- X-IDV-FirstRcvd: diskless6.external [209.120.196.50] 9, 25 -- X-IDV-HELO: webmail.idv.net 9, 25 -- X-IDV-Authenticated-User: cgarber 9, 25 -- Received: from 218.106.175.34 (auth. user cgarber-at-mail.2spi.com) 9, 25 -- by webmail.idv.net with HTTP; Wed, 14 Mar 2007 08:49:47 -0500 9, 25 -- To: microscopy-at-msa.microscopy.com 9, 25 -- Subject: TEM support films for "protein TEM work" 9, 25 -- Date: Wed, 14 Mar 2007 08:49:47 -0500 9, 25 -- X-Mailer: IlohaMail/0.9.0 (On: webmail.idv.net) 9, 25 -- Message-ID: {k8HxdWzS.1173880187.5829640.cgarber-at-mail.2spi.com} 9, 25 -- From: {cgarber-at-2spi.com} 9, 25 -- Bounce-To: {cgarber-at-2spi.com} 9, 25 -- Errors-To: {cgarber-at-2spi.com} 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Type: text/plain; charset=ISO-8859-1 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2EDnlpP007326 ==============================End of - Headers==============================
On Mar 13, 2007, at 9:09 PM, jzheng-at-uci.edu wrote:
} This may be a simple question for those who are working on protein } observed by TEM. What is the best supporting film for protein TEM work, } silicon monoxide grids or ultra thin carbon film grids? If you can } tell me } the reason and the products your are using, it will be very helpful. } Dear Jeffery, To an extent, the preferred support film is specimen and technique dependent. For cryoEM of soluble proteins, a holey film is best, and one images in the holes, so the image has no film in it. For cryoEM of membrane proteins, the thin carbon evaporated on mica--either on a fine-mesh grid or an underlying holey film--works well. I have not compared the results using thin carbon to those using the Si compounds Chuck Garber recommends. For negative stained specimens, a continuous film is necessary, and formvar/carbon works well, since the stain provides large contrast and the effect of the film is insignificant. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Wed Mar 14 11:51:11 2007 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2EGpAeS022650 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 14 Mar 2007 11:51:10 -0500 4, 22 -- Received: from fire-dog.caltech.edu (fire-dog [192.168.1.4]) 4, 22 -- by earth-ox-postvirus (Postfix) with ESMTP id 3CB3936F44 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 14 Mar 2007 09:51:10 -0700 (PDT) 4, 22 -- Received: from [192.168.159.233] (jpix-01.caltech.edu [131.215.2.133]) 4, 22 -- by earth-ox.its.caltech.edu (Postfix) with ESMTP id 7DB1C3703A 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 14 Mar 2007 09:51:05 -0700 (PDT) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v624) 4, 22 -- In-Reply-To: {200703140409.l2E49EH3016398-at-ns.microscopy.com} 4, 22 -- References: {200703140409.l2E49EH3016398-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {5a3bc06cadfcf6a1fe19acfad6af98ff-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] best supporting film for protein TEM 4, 22 -- Date: Wed, 14 Mar 2007 10:00:40 -0700 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.624) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.3.3 ==============================End of - Headers==============================
Without any additional information we would suggest you try the SiO substrates. We produce a lot of SiO substrates and do not always know how they are being used but, anecdotally we believe many of them are being used for protein TEM work.
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==============================Original Headers============================== 13, 27 -- From jd-at-laddresearch.com Wed Mar 14 12:24:38 2007 13, 27 -- Received: from aston.electric.net (aston.electric.net [216.129.90.209]) 13, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2EHOcWZ002053 13, 27 -- for {microscopy-at-microscopy.com} ; Wed, 14 Mar 2007 12:24:38 -0500 13, 27 -- Received: from root by aston.electric.net with emc1-ok (Exim 4.62) 13, 27 -- (envelope-from {jd-at-laddresearch.com} ) 13, 27 -- id 1HRXDJ-00008I-TP; Wed, 14 Mar 2007 10:24:37 -0700 13, 27 -- Received: by emcmailer; Wed, 14 Mar 2007 10:24:37 -0700 13, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 13, 27 -- by aston.electric.net with esmtps (TLSv1:AES256-SHA:256) 13, 27 -- (Exim 4.62) 13, 27 -- (envelope-from {jd-at-laddresearch.com} ) 13, 27 -- id 1HRXDI-0008Pe-Tt; Wed, 14 Mar 2007 10:24:36 -0700 13, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 27 -- Date: Wed, 14 Mar 2007 13:24:10 -0400 13, 27 -- To: jzheng-at-uci.edu 13, 27 -- From: jd {jd-at-laddresearch.com} 13, 27 -- Subject: Re: [Microscopy] best supporting film for protein TEM 13, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 13, 27 -- In-Reply-To: {200703140413.l2E4DgvA027251-at-ns.microscopy.com} 13, 27 -- References: {200703140413.l2E4DgvA027251-at-ns.microscopy.com} 13, 27 -- Mime-Version: 1.0 13, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 13, 27 -- X-Outbound-IP: 216.204.198.170 13, 27 -- X-Env-From: jd-at-laddresearch.com 13, 27 -- X-Virus-Status: Scanned by VirusSMART (c) 13, 27 -- Message-Id: {E1HRXDJ-00008I-TP-at-aston.electric.net} ==============================End of - Headers==============================
Sorry - I accidentally left off the following disclaimer from my previous posting: Ladd Research sells SiO substrates, carbon substrates, support films, grids and associated products
Hi Jeffrey,
Without any additional information we would suggest you try the SiO substrates. We produce a lot of SiO substrates and do not always know how they are being used but, anecdotally we believe many of them are being used for protein TEM work.
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 15, 25 -- From jd-at-laddresearch.com Wed Mar 14 12:29:12 2007 15, 25 -- Received: from aston.electric.net (aston.electric.net [216.129.90.209]) 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2EHTC6d012282 15, 25 -- for {microscopy-at-microscopy.com} ; Wed, 14 Mar 2007 12:29:12 -0500 15, 25 -- Received: from root by aston.electric.net with emc1-ok (Exim 4.62) 15, 25 -- (envelope-from {jd-at-laddresearch.com} ) 15, 25 -- id 1HRXHj-0003Ox-TY; Wed, 14 Mar 2007 10:29:11 -0700 15, 25 -- Received: by emcmailer; Wed, 14 Mar 2007 10:29:11 -0700 15, 25 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 15, 25 -- by aston.electric.net with esmtps (TLSv1:AES256-SHA:256) 15, 25 -- (Exim 4.62) 15, 25 -- (envelope-from {jd-at-laddresearch.com} ) 15, 25 -- id 1HRXHh-0003Gy-Up; Wed, 14 Mar 2007 10:29:10 -0700 15, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 15, 25 -- Date: Wed, 14 Mar 2007 13:28:45 -0400 15, 25 -- To: jzheng-at-uci.edu 15, 25 -- From: jd {jd-at-laddresearch.com} 15, 25 -- Subject: Re: [Microscopy] best supporting film for protein TEM 15, 25 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 15, 25 -- Mime-Version: 1.0 15, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 15, 25 -- X-Outbound-IP: 216.204.198.170 15, 25 -- X-Env-From: jd-at-laddresearch.com 15, 25 -- X-Virus-Status: Scanned by VirusSMART (c) 15, 25 -- Message-Id: {E1HRXHj-0003Ox-TY-at-aston.electric.net} ==============================End of - Headers==============================
submission of abstracts until 10 May 2007 reduced rate registration until 20 June 2007
This conference, sponsored by the International Union of Crystallography, will bring together experts in methods of interrogating structures at nano- and meso-scales, and multiscale materials modelling.
Topics of the conference include:
- electron diffraction imaging - electron spectroscopy - density functional methods - structure of defects and dislocations - dynamical properties of defects - dislocation dynamics - surfaces and defects on surfaces - structure of grain boundaries - atomistic modelling of diffusion of defects - modelling microstructure on nano- and mesoscales - imaging and spectroscopy of nanostructures - magnetic effects in materials - diffuse and small angle scattering - charge density measurements - advanced materials for power generation - applications of electron microscopy - neutron and X-ray diffraction methods
September is the best time for visiting the city of Moscow to enjoy the numerous sights and attractions that it has to offer.
We are looking forward to welcoming you to EMMM-07
Prof. Anatoly Avilov emmm-07-at-ns.crys.ras.ru
Vice Chair, Organizing Committee of EMMM-2007, Institute of Crystallography, Russian Academy of Sciences, Leninsky Prospect, Moscow, Russian Federation
==============================Original Headers============================== 11, 26 -- From marksmsa-at-gmail.com Wed Mar 14 13:44:18 2007 11, 26 -- Received: from nf-out-0910.google.com (nf-out-0910.google.com [64.233.182.186]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2EIiH3u026311 11, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2007 13:44:18 -0500 11, 26 -- Received: by nf-out-0910.google.com with SMTP id a4so333727nfc 11, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2007 11:44:16 -0700 (PDT) 11, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 11, 26 -- d=gmail.com; s=beta; 11, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 11, 26 -- b=OV9HNICAV3vQ1/7xbDEYUxQksASiB97gK41a32sHRF4B/JWKlqHdDzyIJuh6iZmhwWbCeaQaiQe/ida0d76xm4eTbwhODmB84vBAYctmT4NrMf8+OojWns7rsv6jRM0JoWMLbCHgb5GskADfyFoBsqQaPZq8sTwuDAG2vHezg44= 11, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 11, 26 -- d=gmail.com; s=beta; 11, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 11, 26 -- b=Z9n7ijJbW6aDFK6if5J00sF0fHCbEwHXULy45LWPig9dEsuGzLMwfUje6iZrVO96lFzSU2apH+0tai8XOICVkWQl6KnFLrKYFCCVWE2wHsyJyul3L0vjgF6novY3VI19RyGQ5GCiTGq+jQeeRyRayhJ6oY0BNRmH/DOIV5aiYI8= 11, 26 -- Received: by 10.78.136.7 with SMTP id j7mr1379301hud.1173897855459; 11, 26 -- Wed, 14 Mar 2007 11:44:15 -0700 (PDT) 11, 26 -- Received: by 10.78.47.7 with HTTP; Wed, 14 Mar 2007 11:44:15 -0700 (PDT) 11, 26 -- Message-ID: {e13ba6260703141144j3d266273pccd9bce4f2880f03-at-mail.gmail.com} 11, 26 -- Date: Wed, 14 Mar 2007 13:44:15 -0500 11, 26 -- From: "L Marks" {marksmsa-at-gmail.com} 11, 26 -- To: Microscopy-at-microscopy.com 11, 26 -- Subject: EMMM2007, call for papers 11, 26 -- MIME-Version: 1.0 11, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 26 -- Content-Transfer-Encoding: 7bit 11, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
The first 2007 meeting of the Midwest Microscopy and Microanalysis Society, held jointly with the Biological Imaging Facility at Northwestern University in Evanston, IL, will be held on Friday, March 23rd. Please follow the link below and click on Meetings for details of the program and registration information. Note that the M3S website address has changed:
www.midwestmicroscopy.org
We look forward to seeing you at this and future meetings.
Regards,
Elaine Schumacher President, Midwest Microscopy and Microanalysis Society Elaine-at-midwestmicroscopy.org
==============================Original Headers============================== 8, 27 -- From eschumacher-at-mccrone.com Wed Mar 14 15:50:09 2007 8, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2EKo8CP009774 8, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 14 Mar 2007 15:50:09 -0500 8, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 8, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id E42F01A800B 8, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 14 Mar 2007 14:50:09 -0600 (CST) 8, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 8, 27 -- by pgp.mccrone.com (PGP Universal service); 8, 27 -- Wed, 14 Mar 2007 14:50:09 -0600 8, 27 -- X-PGP-Universal: processed 8, 27 -- Content-class: urn:content-classes:message 8, 27 -- MIME-Version: 1.0 8, 27 -- Content-Type: text/plain; 8, 27 -- charset="us-ascii" 8, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 27 -- Subject: Meeting: Midwest Microscopy and Microanalysis Society 8, 27 -- Date: Wed, 14 Mar 2007 15:49:58 -0500 8, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A7B974F4-at-MCCRONEMSG.tmg.mccrone.com} 8, 27 -- X-MS-Has-Attach: 8, 27 -- X-MS-TNEF-Correlator: 8, 27 -- Thread-Topic: Meeting: Midwest Microscopy and Microanalysis Society 8, 27 -- Thread-Index: AcdmelPRH7eFnkpsR1apPoFTyq7bRw== 8, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 8, 27 -- To: {Microscopy-at-MSA.Microscopy.Com} 8, 27 -- Content-Transfer-Encoding: 8bit 8, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2EKo8CP009774 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both schramv-at-mail.nih.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: schramv-at-mail.nih.gov Name: Vincent Schram
Organization: NIH / NICHD
Title-Subject: [Filtered] Zeiss workshop in Bethesda (MD) March 27 - 28
Question: The NICHD Microscopy & Imaging Core is organizing with Carl Zeiss on March 27 and 28 a two-day workshop on deconvolution, long-term incubation and image analysis. The event will be held on the NIH Bethesda campus in Maryland. Anyone using or planning to use a conventional fluorescence microscope is invited to attend.
The workshop includes seminars held in B49 / rm 1A51 and equipment demonstration in B49 / rm 6C72. Please contact Ruth Redman (rredman-at-zeiss.com) to schedule time on the equipment.
The full program is below:
3/27- 10-11AM LECTURE: Wide field Optical Sectioning Techniques for Fluorescent Specimens: Structured Illumination with Apotome and 3D Deconvolution Demonstrations of these techniques can be scheduled on a hourly basis from 11:30-4:30 that afternoon in B49 / rm 6C72.
3/28- 10-11AM LECTURE: High Speed Physiological Imaging and Analysis with Long Term Incubation General demonstration immediately following
3/28 1-2PM LECTURE: Automated Image Analysis and Particle Tracking You are invited to bring your own images for analysis following the lecture.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stevem-at-vt.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: stevem-at-vt.edu Name: Stephen McCartney
Organization: VaTech University
Title-Subject: [Filtered] job posting
Question: The following position is open at Va Tech:
Research Associate/Senior Research Associate, Institute for Critical Technology & Applied Sciences.
Posting Number 070224
Operate instrumentation in the Nanoscale Characterization and Fabrication Laboratory. Maintain and operate the FEI Titan Transmission Electron Microscope (TEM) and the Helios Nanolab 600 Focused Ion Beam (FIB) in the NCFL.
Use this link, click on 'Search and Apply for Faculty Staff and Wage Positions' then use the above Posting number for more info.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mgb-at-ansto.gov.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mgb-at-ansto.gov.au Name: Mark Blackford
Organization: ANSTO
Title-Subject: [Filtered] TEM aperture questions
Question: Hi All,
A colleague is developing a DigitalMicrograph script to help align our JEOL 2010F TEM. This requires a selected area aperture which is as close to circular as possible (better than 0.5%) to provide a reference image.
Can anyone tell me how TEM apertures are manufactured? How close to a perfect circle can they be made?
I have exactly the same question. I am trying to find a database that will coordinate the cataloguing of images from SEM, confocal and light microscopes from a large number of regular and temporary users. The software not only needs to catalogue the images but also accommodate updateable links to electronic 'notebooks' which describe experimental details and results (which can be accessed over a network and edited by people associated with the project). It is also preferable to database such things as linked PDFs (relevant literature) and data files such as Excel spreadsheets.
I have so far not managed to find one single program that can do all of this! There's a great database program called Pax-It, but this is a little bit user-unfriendly (one important stipulation is that the program needs to be user-friendly since it would be used by a large mix of people with a gradation of computer literacy) and so far I can't see any ability to database together with electronic notebooks. But I may be asking too much? If anyone could help with this, I would be very happy to hear from you (and Paul as well).
Thanks,
Mark
Dr. Mark Talbot CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia mark.talbot-at-csiro.au
ph. 61 (0)2-6246 5256
fax. 61 (0)2-6246 5334
-----Original Message----- X-from: PWebster-at-hei.org [mailto:PWebster-at-hei.org] Sent: Thursday, 8 March 2007 4:42 AM To: Talbot, Mark (PI, Black Mountain)
Hi,
We need a way to catalog stored images (EM & LM) together with experimental data (text) for access by in-house researchers as well as off-site users.
Does anyone know of any commercial products available that would fit our needs?
We have someone here who would like to try to develop this but it will be a waste of time and money if something is already available.
Regards,
Paul.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
==============================Original Headers============================== 10, 18 -- From PWebster-at-hei.org Wed Mar 7 11:37:08 2007 10, 18 -- Received: from hi0sml1.hei.org (House-Ear-Institute-1114861.cust-rtr.pacbell.net [71.136.31.242]) 10, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l27Hb7C7009704 10, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Mar 2007 11:37:08 -0600 10, 18 -- Received: from 10.10.42.96 ([10.10.42.96]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 10, 18 -- Wed, 7 Mar 2007 17:37:07 +0000 10, 18 -- User-Agent: Microsoft-Entourage/11.3.3.061214 10, 18 -- Date: Wed, 07 Mar 2007 09:37:06 -0800 10, 18 -- Subject: EM & LM Digital database 10, 18 -- From: "Webster, Paul" {PWebster-at-hei.org} 10, 18 -- To: {Microscopy-at-Microscopy.Com} 10, 18 -- Message-ID: {C2143842.FBF9%PWebster-at-hei.org} 10, 18 -- Thread-Topic: EM & LM Digital database 10, 18 -- Thread-Index: Acdg3zlheCaVzszSEduDkgANk7Zh7g== 10, 18 -- Mime-version: 1.0 10, 18 -- Content-type: text/plain; 10, 18 -- charset="US-ASCII" 10, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 26, 29 -- From mark.talbot-at-csiro.au Wed Mar 14 23:26:27 2007 26, 29 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au [150.229.7.37]) 26, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2F4QQbo030308 26, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2007 23:26:26 -0500 26, 29 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=fwsYT0BHKV9uRk3G2Zb9vXZzBv50fmCgoBpKe7raTqZcQ0qgmGrpUUXWToE6c+4dSEhBfr1Asv4ExfnNdtE5HitW3rhFLOXGI/QAtVUxglK3mxy8fGN5tmVFK8r2eyBE; 26, 29 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 26, 29 -- by act-ironport-int.csiro.au with ESMTP; 15 Mar 2007 15:26:26 +1100 26, 29 -- X-IronPort-AV: i="4.14,287,1170594000"; 26, 29 -- d="scan'208"; a="148460283:sNHT38399564" 26, 29 -- Received: from exnswn1-syd.nexus.csiro.au ([130.155.3.31]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 26, 29 -- Thu, 15 Mar 2007 15:26:25 +1100 26, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 26, 29 -- content-class: urn:content-classes:message 26, 29 -- MIME-Version: 1.0 26, 29 -- Content-Type: text/plain; 26, 29 -- charset="us-ascii" 26, 29 -- Subject: RE: [Microscopy] EM & LM Digital database 26, 29 -- Date: Thu, 15 Mar 2007 15:26:25 +1100 26, 29 -- Message-ID: {BF94750921492E4AA5B825026FCE6A240148A07E-at-exnswn1-syd.nexus.csiro.au} 26, 29 -- X-MS-Has-Attach: 26, 29 -- X-MS-TNEF-Correlator: 26, 29 -- Thread-Topic: [Microscopy] EM & LM Digital database 26, 29 -- Thread-Index: Acdg3+YELiupnji8T5ObR1cNPxj8XgAY6BYg 26, 29 -- References: {200703071741.l27Hfq0E022243-at-ns.microscopy.com} 26, 29 -- From: {mark.talbot-at-csiro.au} 26, 29 -- To: {Microscopy-at-microscopy.com} 26, 29 -- X-OriginalArrivalTime: 15 Mar 2007 04:26:25.0357 (UTC) FILETIME=[17DBA7D0:01C766BA] 26, 29 -- Content-Transfer-Encoding: 8bit 26, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2F4QQbo030308 ==============================End of - Headers==============================
Molybdenum and platinum apertures are drilled and thin foil apertures are made with a deposition process using a mask. We regularly perform SEM inspections on all types of apertures and measure for things like roundness and concentricity. From our experience, I can tell you that getting any aperture or apertures that will meet your spec is going to be hit or miss, but probably more miss. At a maximum 1/2% variation for roundness, just measuring it presents a challenge.
Sorry I don't have better news.
Best of luck, Mike Nesta
-- Michael R. Nesta Managing Director Energy Beam Sciences, Inc. Tel: 860 653-0411 Fax: 860 653-0422 MNesta-at-ebsciences.com www.ebsciences.com “ADDING BRILLIANCE TO YOUR VISION”
mgb-at-ansto.gov.au wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mgb-at-ansto.gov.au as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mgb-at-ansto.gov.au } Name: Mark Blackford } } Organization: ANSTO } } Title-Subject: [Filtered] TEM aperture questions } } Question: Hi All, } } A colleague is developing a DigitalMicrograph script to help align } our JEOL 2010F TEM. This requires a selected area aperture which is } as close to circular as possible (better than 0.5%) to provide a } reference image. } } Can anyone tell me how TEM apertures are manufactured? } How close to a perfect circle can they be made? } } Cheers, } } Mark Blackford } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 10, 12 -- From zaluzec-at-microscopy.com Wed Mar 14 22:58:31 2007 } 10, 12 -- Received: from [192.168.2.1] ([206.69.208.26]) } 10, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2F3wVk2018640 } 10, 12 -- for {microscopy-at-microscopy.com} ; Wed, 14 Mar 2007 22:58:31 -0500 } 10, 12 -- Mime-Version: 1.0 } 10, 12 -- X-Sender: (Unverified) } 10, 12 -- Message-Id: {p06020402c21e76805813-at-[192.168.2.1]} } 10, 12 -- Date: Wed, 14 Mar 2007 23:06:28 -0500 } 10, 12 -- To: microscopy-at-microscopy.com } 10, 12 -- From: mgb-at-ansto.gov.au (by way of MicroscopyListserver) } 10, 12 -- Subject: viaWWW: TEM aperture questions } 10, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 26 -- From mnesta-at-ebsciences.com Thu Mar 15 08:24:15 2007 9, 26 -- Received: from mx-outbound01.easydns.com (mailout.easydns.com [205.210.42.54]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2FDOFHM020698 9, 26 -- for {microscopy-at-microscopy.com} ; Thu, 15 Mar 2007 08:24:15 -0500 9, 26 -- Received: from mail.ebsciences.com (unknown [69.182.224.2]) 9, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 9, 26 -- (No client certificate requested) 9, 26 -- by mx-outbound01.easydns.com (Postfix) with ESMTP id 226B67FBD; 9, 26 -- Thu, 15 Mar 2007 09:22:21 -0400 (EDT) 9, 26 -- Received: from vtelinet-209-134-41-220.vermontel.net ([209.134.41.220] helo=[192.168.1.5]) 9, 26 -- by mail.ebsciences.com with esmtpsa (TLSv1:AES256-SHA:256) 9, 26 -- (Exim 4.62) 9, 26 -- (envelope-from {mnesta-at-ebsciences.com} ) 9, 26 -- id 1HRpw9-0003RL-7F; Thu, 15 Mar 2007 08:24:09 -0500 9, 26 -- Message-ID: {45F9490B.7080409-at-ebsciences.com} 9, 26 -- Date: Thu, 15 Mar 2007 09:24:27 -0400 9, 26 -- From: Mike Nesta {mnesta-at-ebsciences.com} 9, 26 -- Organization: Energy Beam Sciences 9, 26 -- User-Agent: Thunderbird 1.5.0.10 (Windows/20070221) 9, 26 -- MIME-Version: 1.0 9, 26 -- To: mgb-at-ansto.gov.au, microscopy-at-microscopy.com 9, 26 -- Subject: Re: [Microscopy] viaWWW: TEM aperture questions 9, 26 -- References: {200703150404.l2F4432c023676-at-ns.microscopy.com} 9, 26 -- In-Reply-To: {200703150404.l2F4432c023676-at-ns.microscopy.com} 9, 26 -- Content-Type: text/plain; charset=windows-1252; format=flowed 9, 26 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
we are running the Zeiss Axio Vision 3.1 software on WindowsNT Workstation. Now, we would like to install Windows XP on our system. Does anybody know if Axio Vision 3.1 will still run on WinXP? Any hints will be welcomed.
Best wishes
Pascal
-- Pascal Lorentz Institute of Biochemistry and Genetics Department of Clinical-Biological Sciences University of Basel Mattenstrasse 28 4058 Basel Switzerland
Hello: I am looking for information on an optical system that can take 3D images and also measure X-Y and Z. thanks. Steve
Stephen McCartney Senior Research Associate Macromolecules and Interfaces Institute 2108 Hahn Hall Va Tech Blacksburg, VA 24061 540-231-9765 - phone 540-231-8517 - FAX
==============================Original Headers============================== 3, 20 -- From stmccart-at-vt.edu Thu Mar 15 11:56:02 2007 3, 20 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu [198.82.162.213]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2FGu21V016239 3, 20 -- for {microscopy-at-microscopy.com} ; Thu, 15 Mar 2007 11:56:02 -0500 3, 20 -- Received: from dagger.cc.vt.edu (IDENT:mirapoint-at-evil-dagger.cc.vt.edu [10.1.1.11]) 3, 20 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id l2FGtmwM015541 3, 20 -- for {microscopy-at-microscopy.com} ; Thu, 15 Mar 2007 12:56:01 -0400 3, 20 -- Received: from mri-system.vt.edu (h80ada7f8.dhcp.vt.edu [128.173.167.248]) 3, 20 -- by dagger.cc.vt.edu (MOS 3.8.2-GA) 3, 20 -- with ESMTP id HHL09434; 3, 20 -- Thu, 15 Mar 2007 12:56:01 -0400 (EDT) 3, 20 -- Message-Id: {5.2.1.1.0.20070315125409.05347588-at-pop.vt.edu} 3, 20 -- X-Sender: stmccart-at-pop.vt.edu 3, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 3, 20 -- Date: Thu, 15 Mar 2007 12:56:01 -0400 3, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 20 -- From: Stephen McCartney {stmccart-at-vt.edu} 3, 20 -- Subject: 3d optical microscope 3, 20 -- Mime-Version: 1.0 3, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Bernard Friedman Memorial Workshop Polarized Light Microscopy May 5, 12, 19 & 26, 2007 An advanced course on polarized light microscopy which will cover the following topics: The nature of polarized light The origin and interpretation of interference colors Birefringence and crystal orientation, The Indicatrix Compensation and variable compensators Interference figures and their interpretation The workshop will consist of four consecutive Saturdays of lectures and hands on labs to cover the theoretical and practical aspects of polarized light microscopy. The course instructors include Jan Hinsch formerly of Leica Microsystems, Mary McCann of McCann Imaging, John Reffner of Smiths Detection and N.Y.M.S. Instructor Don O'Leary. WHEN: May 5, 12, 19 & 26, 2006 from 10 A.M. to 4 P.M. WHERE: 30 North Mountain Avenue, Montclair, NJ 07042. Phone (973) 744-0043 COST: $395 for N.Y.M.S. members, $425 for non-members (includes membership). Lunch and course materials are included. Checks made out to N.Y.M.S. WHO: Advanced course for those who have completed "The Use of the Microscope" or are experienced in microscopy and familiar with the theory of its use. HOW: Register using the form below. Limited to the first 12 registrants. Return form to Don O'Leary, 10 Sampson Street, Unit 113, Saddle Brook, NJ 07663 FURTHER INFORMATION: Call D. O'Leary (201)368-8849 e-mail dkoleary-at-verizon.net PLEASE POST --------------------------------------------------------------------------------------------------------------------------- Registration Form, Polarized Light Microscopy N.Y.M.S. Member_________________ ($395) Non-Member__________($425) Name_____________________________________________________________ Address___________________________________________________________ City__________________State____________ZIP______________ Phone (W)_________________________(H)______________________________ e-mail________________________________________
==============================Original Headers============================== 2, 17 -- From dkoleary-at-verizon.net Thu Mar 15 13:00:35 2007 2, 17 -- Received: from web84001.mail.mud.yahoo.com (web84001.mail.mud.yahoo.com [68.142.206.171]) 2, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2FI0ZuN029124 2, 17 -- for {Microscopy-at-sparc5.microscopy.com} ; Thu, 15 Mar 2007 13:00:35 -0500 2, 17 -- Received: (qmail 83378 invoked by uid 60001); 15 Mar 2007 18:00:34 -0000 2, 17 -- X-YMail-OSG: Ykx1SPIVM1kDbKyasxZ1NBMbSMTUKiQ5wAERtE6JNOWJUV10dHEjurIVhYhBPcFTcZ3YlacdkyISUQgWl7rZazqs6LQhdhYT5ECn8_UOSwAw8pTTTy2XnuwIGmpCMYX5tyVFNb7DKkTLn7cTB9HNTTHmTw-- 2, 17 -- Received: from [71.122.14.7] by web84001.mail.mud.yahoo.com via HTTP; Thu, 15 Mar 2007 11:00:34 PDT 2, 17 -- X-Mailer: YahooMailRC/368.8 YahooMailWebService/0.6.132.8 2, 17 -- Date: Thu, 15 Mar 2007 11:00:34 -0700 (PDT) 2, 17 -- From: DONALD OLEARY {dkoleary-at-verizon.net} 2, 17 -- Subject: LM: Polarized Light Microscopy workshop 2, 17 -- To: Microscopy List Server {Microscopy-at-ns.microscopy.com} 2, 17 -- MIME-Version: 1.0 2, 17 -- Content-Type: text/plain; charset=iso-8859-1 2, 17 -- Message-ID: {816775.72247.qm-at-web84001.mail.mud.yahoo.com} 2, 17 -- Content-Transfer-Encoding: 8bit 2, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2FI0ZuN029124 ==============================End of - Headers==============================
As there seems a lot of interest in this, I have coped below a section on pp 865 of the 3rd Edition of the Handbook of Biological Confocal Microscopy (Springer, 2005) that might be of some use.
It comes from Chapter 50 on "Databases for Two- and Three-Dimensional Microscopical Images in Biology" by Steffen Lindek, Nicholas J. Salmon, and Ernst H.K. Stelzer.
I trust they will approve.
I personally have no knowledge of this topic.
BioImage BioImage is a database of multi-dimensional digital images for the life sciences that at the time of writing is being restructured but will soon accept image data from a variety of instruments (from microscopy to satellite remote sensing) relating to all aspects of biology (from ultrastructural biology to wildlife conservation). In its first phase (1996-1999), six European research groups and two industrial partners collaborated on a publicly-funded project investigating the possibility of storing, in a single database, data generated from very different specimens (ranging in size from whole biological organisms down to macromolecules) using very different microscopes (light, electron, and atomic force microscopes, etc.). The aim was to design a database system providing hitherto unprecedented levels of comparison and data access to emphasize different, and complementary structural aspects of similar objects. During the transitional period that followed (2000-2001), the consortium partners looked for a new orientation of the database that might lead to a sustainable business model. This led to the integration of BioImage into the ORIEL project (2002-2004): Online Research Information Environment for the Life Sciences (http://www.oriel.org), an EC-funded E-BioSci research project to integrate internet-based biological information resources for the scientific community. Because E-BioSci embraces all life sciences topics, this meant expanding the scope of the BioImage Database to cover non-microscopical image data such as wild-life photography, behavioral biology, ecology, etc. At the same time, the ability to store detailed technical information was reduced.
Cheers,
Jim P.
********************************************** Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU 3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver Canada Info: http://www.3dcourse.ubc.ca/ Applications still being accepted. "If it ain't diffraction, it must be statistics." Anon.
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-- ********************************************** Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU 3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver Canada Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2007 "If it ain't diffraction, it must be statistics." Anon.
==============================Original Headers============================== 12, 27 -- From jbpawley-at-wisc.edu Thu Mar 15 13:01:43 2007 12, 27 -- Received: from adsum.doit.wisc.edu (adsum.doit.wisc.edu [144.92.197.210]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2FI1gZh031081 12, 27 -- for {microscopy-at-microscopy.com} ; Thu, 15 Mar 2007 13:01:42 -0500 12, 27 -- Received: from avs-daemon.smtpauth1.wiscmail.wisc.edu by 12, 27 -- smtpauth1.wiscmail.wisc.edu 12, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 12, 27 -- id {0JEY00403I2UGJ00-at-smtpauth1.wiscmail.wisc.edu} for 12, 27 -- microscopy-at-microscopy.com; Thu, 15 Mar 2007 13:01:42 -0500 (CDT) 12, 27 -- Received: from [144.92.238.207] by smtpauth1.wiscmail.wisc.edu 12, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 12, 27 -- with ESMTPSA id {0JEY001E1I2SFJ10-at-smtpauth1.wiscmail.wisc.edu} ; Thu, 12, 27 -- 15 Mar 2007 13:01:40 -0500 (CDT) 12, 27 -- Date: Thu, 15 Mar 2007 13:01:26 -0500 12, 27 -- From: James Pawley {jbpawley-at-wisc.edu} 12, 27 -- Subject: [Microscopy] RE: EM & LM Digital database 12, 27 -- In-reply-to: {200703150430.l2F4UvOb008596-at-ns.microscopy.com} 12, 27 -- To: mark.talbot-at-csiro.au, Microscopy Listserver {microscopy-at-microscopy.com} 12, 27 -- Message-id: {p06240800c21f3890b1ef-at-[144.92.238.207]} 12, 27 -- MIME-version: 1.0 12, 27 -- Content-type: text/plain; charset=us-ascii; format=flowed 12, 27 -- Content-transfer-encoding: 7BIT 12, 27 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=144.92.238.207 12, 27 -- X-Spam-PmxInfo: Server=avs-5, Version=5.2.1.279297, 12, 27 -- Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.3.15.104934, 12, 27 -- SenderIP=144.92.238.207 12, 27 -- References: {200703150430.l2F4UvOb008596-at-ns.microscopy.com} ==============================End of - Headers==============================
An aperture with a circularity tolerance of 0.5% can be done. We micro-machine most of the standard EM/FIB apertures because they need to be burr and flashing free.
With a tolerance of 0.5% you'd have a substantial loss rate so moly, TA or a metal other than platinum might be a better choice to reduce the costs.
Depending on your hole size requirements we have other techniques we could use. We now do apertures/slits for xenon-ion propulsion satellites. These require extremely tight tolerances and we do them with a proprietary technique. If you want to send me a drawing we can let you know it it's possible.
Disclaimer: Ladd manufactures apertures, pinholes, micro-holes, slits, etc.
At 12:08 AM 3/15/2007, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 12, 27 -- From jd-at-laddresearch.com Thu Mar 15 14:17:28 2007 12, 27 -- Received: from arrows.electric.net (arrows.electric.net [216.129.90.200]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2FJHRb0020826 12, 27 -- for {microscopy-at-microscopy.com} ; Thu, 15 Mar 2007 14:17:27 -0500 12, 27 -- Received: from root by arrows.electric.net with emc1-ok (Exim 4.62) 12, 27 -- (envelope-from {jd-at-laddresearch.com} ) 12, 27 -- id 1HRvS0-0002b3-W3; Thu, 15 Mar 2007 12:17:25 -0700 12, 27 -- Received: by emcmailer; Thu, 15 Mar 2007 11:17:24 -0800 12, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 12, 27 -- by arrows.electric.net with esmtps (TLSv1:AES256-SHA:256) 12, 27 -- (Exim 4.62) 12, 27 -- (envelope-from {jd-at-laddresearch.com} ) 12, 27 -- id 1HRvRz-0002Za-VE; Thu, 15 Mar 2007 12:17:24 -0700 12, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 12, 27 -- Date: Thu, 15 Mar 2007 15:17:12 -0400 12, 27 -- To: mgb-at-ansto.gov.au 12, 27 -- From: jd {jd-at-laddresearch.com} 12, 27 -- Subject: Re: [Microscopy] viaWWW: TEM aperture questions 12, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 12, 27 -- In-Reply-To: {200703150408.l2F48eQX029669-at-ns.microscopy.com} 12, 27 -- References: {200703150408.l2F48eQX029669-at-ns.microscopy.com} 12, 27 -- Mime-Version: 1.0 12, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 12, 27 -- X-Outbound-IP: 216.204.198.170 12, 27 -- X-Env-From: jd-at-laddresearch.com 12, 27 -- X-Virus-Status: Scanned by VirusSMART (c) 12, 27 -- Message-Id: {E1HRvS0-0002b3-W3-at-arrows.electric.net} ==============================End of - Headers==============================
We routinely use 50C for 24 hrs. No problem - must be polymerized in airtight containers though. We've only used gelatin capsules, but I think there is a PCR microfuge tube we tried a long time ago that also worked. There are commercial capsules that are more transparent for UV polymerization.
Any LR White exposed to air (oxygen) will not polymerize, so even in the gelatin capsule, where the lid will have a small bubble, there will be a small amount of liquid to remove.
The other method mentioned above - if the antigen appears to be sensitive to heating and no labeling occurs, you can try to "cold" polymerize by placing the material in capsules that are UV transparent. Place in a container with dry ice and a UV bulb. There are several commercial companies that produce these special beer coolers with fans and lights and reflectors for even polymerization, etc...
John Shields Univ. of Georgia EM Lab
---- Original message ---- } Date: Mon, 12 Mar 2007 10:26:14 -0500 } From: rcommon-at-msu.edu } Subject: [Microscopy] LR White polymerization } To: jpshield-at-uga.edu } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
706-542-4080
==============================Original Headers============================== 7, 21 -- From jpshield-at-uga.edu Thu Mar 15 14:59:22 2007 7, 21 -- Received: from puntd5.cc.uga.edu (puntd5.cc.uga.edu [128.192.1.108]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2FJxMZu011683 7, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 15 Mar 2007 14:59:22 -0500 7, 21 -- Received: from punts4.cc.uga.edu (punts4.cc.uga.edu [128.192.1.115]) 7, 21 -- by puntd5.cc.uga.edu (MOS 3.7.4b-GA) 7, 21 -- with ESMTP id BBW03825; 7, 21 -- Thu, 15 Mar 2007 15:59:20 -0400 (EDT) 7, 21 -- Received: (from punts4.cc.uga.edu [63.111.81.131]) 7, 21 -- by punts4.cc.uga.edu (MOS 3.7.4c) 7, 21 -- with HTTPS/1.1 id ARM80353 (AUTH jpshield-at-uga.edu); 7, 21 -- Thu, 15 Mar 2007 15:59:19 -0400 (EDT) 7, 21 -- From: John Shields {jpshield-at-uga.edu} 7, 21 -- Subject: Re: [Microscopy] LR White polymerization 7, 21 -- To: rcommon-at-msu.edu, msa listserve {Microscopy-at-MSA.Microscopy.Com} 7, 21 -- X-Mailer: Mirapoint Webmail Direct 3.7.4c 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: text/plain; charset=us-ascii 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- Message-Id: {20070315155919.ARM80353-at-punts4.cc.uga.edu} 7, 21 -- Date: Thu, 15 Mar 2007 15:59:19 -0400 (EDT) ==============================End of - Headers==============================
Don't know why this was just rejected as spam; no bad words, and not selling anything.
I'll try reformatting it w/o all the prior stuff.
b.
Hi Justin,
Strictly fwiw: Regarding the " Fun Bit ".
There really isn't a lot to these things if my old Hummer II is at all representative.
When I got mine at a yard sale, I immediately converted it into a simple sputtering system for doing some micro-lithographic jewelry I used to make years ago. www.refractal.com Eventually, I built a proper system, but still have the Hummer around somewhere, and planning to use it someday for teaching.
When all is said and done, a small roughing pump, TC gauge, neon transformer, and an egg timer is all you need if you already have the chamber. Send me a picture of what you have, and I'll help you with the rest.
You may have most of the bits lying around somewhere already.
Cheers,
b.
Rick Becker Cluster Sciences, L.L.C. Borolene Metamaterials 39 Topsfield Rd. Ipswich, MA 01938 US 978-337-9009 ionsourcerer-at-mac.com
If you don't know where you are going, call it "exploration". If you don't know what you are doing, call it "research".
On Mar 13, 2007, at 11:51 PM, gary-at-gaugler.com wrote:
--| --| --| --| ---------------------------------------------------------------------- --| ------ --| The Microscopy ListServer -- CoSponsor: The Microscopy Society of --| America --| To Subscribe/Unsubscribe -- http://www.microscopy.com/ --| MicroscopyListserver --| On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html --| ---------------------------------------------------------------------- --| ------ --| --| I'm puzzled by this. --| --| I posted for specimens for payment and got no responses. --| --| Should I have posted for them as free? I seek bio --| specimens (bacteria, parasites) and I will pay for them. --| I do not have CPD and HMDS, etc. capability. If free --| is better and more successful than paid, let me know. --| --| gary g.
==============================Original Headers============================== 20, 25 -- From ionsourcerer-at-mac.com Thu Mar 15 15:41:14 2007 20, 25 -- Received: from smtpout.mac.com (smtpout.mac.com [17.250.248.173]) 20, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2FKfEtO023847 20, 25 -- for {microscopy-at-microscopy.com} ; Thu, 15 Mar 2007 15:41:14 -0500 20, 25 -- Received: from mac.com (smtpin05-en2 [10.13.10.150]) 20, 25 -- by smtpout.mac.com (Xserve/smtpout03/MantshX 4.0) with ESMTP id l2FKfEOU003647 20, 25 -- for {microscopy-at-microscopy.com} ; Thu, 15 Mar 2007 13:41:14 -0700 (PDT) 20, 25 -- Received: from [192.168.1.2] (pool-72-93-106-133.bstnma.fios.verizon.net [72.93.106.133]) 20, 25 -- (authenticated bits=0) 20, 25 -- by mac.com (Xserve/smtpin05/MantshX 4.0) with ESMTP id l2FKfAw2026360 20, 25 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO); 20, 25 -- Thu, 15 Mar 2007 13:41:12 -0700 (PDT) 20, 25 -- In-Reply-To: {200703152033.l2FKXYav023743-at-ns.microscopy.com} 20, 25 -- References: {200703152033.l2FKXYav023743-at-ns.microscopy.com} 20, 25 -- Mime-Version: 1.0 (Apple Message framework v752.2) 20, 25 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 20, 25 -- Message-Id: {0E3E9352-D655-4424-B6E7-3A03D3B6019C-at-mac.com} 20, 25 -- Content-Transfer-Encoding: 7bit 20, 25 -- From: Rick Becker {ionsourcerer-at-mac.com} 20, 25 -- Subject: Home-brew Coater - The Fun Bit 20, 25 -- Date: Thu, 15 Mar 2007 16:41:10 -0400 20, 25 -- To: microscopy-at-microscopy.com 20, 25 -- X-Mailer: Apple Mail (2.752.2) 20, 25 -- X-Brightmail-Tracker: AAAAAA== 20, 25 -- X-Brightmail-scanned: yes ==============================End of - Headers==============================
Hi, There, This position was posted on Feb 7. You still have a very short period of time to apply it. You should contact Ms. Dorothy Miles instead of me. If you have applied it, your documents should be properly filed. You will be informed in due time. Best regards, Jian-Guo
Materials Characterization Specialist University of California, Irvine Salary: Commensurate with experience
The University of California, Irvine is seeking a materials characterization specialist to work in the campus-wide Nanomaterials Characterization and Fabrication Facility (NCF2). The successful candidate is expected to have an earned PhD degree in a relevant field, possess an extensive knowledge in analytical instrumentation (SEM, XRD, AFM, TEM, FTIR, TGA, DSC) and research implementation, and have rich experience in sample preparation. He/she should have either extensive knowledge of techniques and protocols in soft materials characterization, or demonstrate a desire to acquire such knowledge. The applicant should also have excellent writing and inter-personal communication skills, and strong team spirit. Good computing skills are also desirable. The materials characterization specialist's responsibilities include carrying out day-to-day operations of analytical equipments including, but not limited to, SEM, XRD, TEM and AFM/STM. He/she will also be responsible for (1) maintaining all instruments in good working condition, (2) training and helping users in the use of analytical equipments, (3) preparing samples and providing help to users in sample preparation, (4) assisting in courses related to analytical instrumentation, (5) conducting service for off-campus and industrial users, (6) maintaining the facility infrastructure, and (7) carrying out miscellaneous facility-related tasks assigned by the facility director. The specialist will report to the director of NCF2. Salary is commensurate with experience. This position will open immediately and remain open until filled.
Please include in the application the following materials: • Curriculum Vita • 2-3 publications • Three letters of recommendation letters (may be submitted shortly after the submission of the application)
Ms. Dorothy Miles 4100 Calit2 Bldg Irvine, CA 92697-2800 djmiles-at-uci.edu ph: 949/824-5178 fax: 949/824-4403
The University of California, Irvine is an equal opportunity employer committed to excellence through diversity.
==============================Original Headers============================== 8, 21 -- From jzheng-at-uci.edu Fri Mar 16 00:20:56 2007 8, 21 -- Received: from relay2.es.uci.edu (relay2.es.uci.edu [128.200.80.28]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2G5KtG3013639 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 00:20:56 -0500 8, 21 -- Received: from webmail.uci.edu (webmail7.es.uci.edu [128.200.80.187]) 8, 21 -- by relay2.es.uci.edu (8.13.1/8.13.1) with ESMTP id l2G5KsYM017290 8, 21 -- for {microscopy-at-microscopy.com} ; Thu, 15 Mar 2007 22:20:55 -0700 8, 21 -- Received: from 128.195.177.193 8, 21 -- (SquirrelMail authenticated user jzheng) 8, 21 -- by webmail.uci.edu with HTTP; 8, 21 -- Thu, 15 Mar 2007 22:20:55 -0700 (PDT) 8, 21 -- Message-ID: {2506.128.195.177.193.1174022455.squirrel-at-webmail.uci.edu} 8, 21 -- Date: Thu, 15 Mar 2007 22:20:55 -0700 (PDT) 8, 21 -- Subject: Materials Characterization Specialist position at University of 8, 21 -- California, Irvine 8, 21 -- From: jzheng-at-uci.edu 8, 21 -- To: microscopy-at-microscopy.com 8, 21 -- User-Agent: SquirrelMail/1.4.9a 8, 21 -- MIME-Version: 1.0 8, 21 -- Content-Type: text/plain;charset=iso-8859-1 8, 21 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Has anyone tried the Open Microscopy Environment database system? It is freeware opensource Linux server based software, and works with ImagJ and commercial image analysis plugins, plus "the Java-based server system is for visualizing, managing, and annotating microscope images and metadata". See:
http://openmicroscopy.org/
"OME is a collaborative effort among academic labs and a number of commercial entities. All OME formats are open and available for use by the community. All OME source code is available under the GNU library general public license (LGPL), but OME is designed to interact with new and existing commercial software".
I saw a presentation on the software a few years ago and it seemed quite good, but no-one here is really interested in sharing images on this scale. I wondered if anyone has any experience to share on the suitability of this open-source software for a Biology optical microscope facility (it seems literally made for this, but requires a free PC to convert to a Linux server).
Any thoughts?
Regards
Keith
--------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Institute of Ophthalmology UCL, London EC1V 9EL
-----Original Message----- X-from: jbpawley-at-wisc.edu [mailto:jbpawley-at-wisc.edu] Sent: 15 March 2007 18:05 To: keith.morris-at-ucl.ac.uk
Hi all,
As there seems a lot of interest in this, I have coped below a section on pp 865 of the 3rd Edition of the Handbook of Biological Confocal Microscopy (Springer, 2005) that might be of some use.
It comes from Chapter 50 on "Databases for Two- and Three-Dimensional Microscopical Images in Biology" by Steffen Lindek, Nicholas J. Salmon, and Ernst H.K. Stelzer.
I trust they will approve.
I personally have no knowledge of this topic.
BioImage BioImage is a database of multi-dimensional digital images for the life sciences that at the time of writing is being restructured but will soon accept image data from a variety of instruments (from microscopy to satellite remote sensing) relating to all aspects of biology (from ultrastructural biology to wildlife conservation). In its first phase (1996-1999), six European research groups and two industrial partners collaborated on a publicly-funded project investigating the possibility of storing, in a single database, data generated from very different specimens (ranging in size from whole biological organisms down to macromolecules) using very different microscopes (light, electron, and atomic force microscopes, etc.). The aim was to design a database system providing hitherto unprecedented levels of comparison and data access to emphasize different, and complementary structural aspects of similar objects. During the transitional period that followed (2000-2001), the consortium partners looked for a new orientation of the database that might lead to a sustainable business model. This led to the integration of BioImage into the ORIEL project (2002-2004): Online Research Information Environment for the Life Sciences (http://www.oriel.org), an EC-funded E-BioSci research project to integrate internet-based biological information resources for the scientific community. Because E-BioSci embraces all life sciences topics, this meant expanding the scope of the BioImage Database to cover non-microscopical image data such as wild-life photography, behavioral biology, ecology, etc. At the same time, the ability to store detailed technical information was reduced.
Cheers,
Jim P.
********************************************** Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU 3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver Canada Info: http://www.3dcourse.ubc.ca/ Applications still being accepted. "If it ain't diffraction, it must be statistics." Anon.
} --------------------------------------------------------------------------- - } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- ********************************************** Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU 3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver Canada Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2007 "If it ain't diffraction, it must be statistics." Anon.
==============================Original Headers============================== 12, 27 -- From jbpawley-at-wisc.edu Thu Mar 15 13:01:43 2007 12, 27 -- Received: from adsum.doit.wisc.edu (adsum.doit.wisc.edu [144.92.197.210]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2FI1gZh031081 12, 27 -- for {microscopy-at-microscopy.com} ; Thu, 15 Mar 2007 13:01:42 -0500 12, 27 -- Received: from avs-daemon.smtpauth1.wiscmail.wisc.edu by 12, 27 -- smtpauth1.wiscmail.wisc.edu 12, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 12, 27 -- id {0JEY00403I2UGJ00-at-smtpauth1.wiscmail.wisc.edu} for 12, 27 -- microscopy-at-microscopy.com; Thu, 15 Mar 2007 13:01:42 -0500 (CDT) 12, 27 -- Received: from [144.92.238.207] by smtpauth1.wiscmail.wisc.edu 12, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 12, 27 -- with ESMTPSA id {0JEY001E1I2SFJ10-at-smtpauth1.wiscmail.wisc.edu} ; Thu, 12, 27 -- 15 Mar 2007 13:01:40 -0500 (CDT) 12, 27 -- Date: Thu, 15 Mar 2007 13:01:26 -0500 12, 27 -- From: James Pawley {jbpawley-at-wisc.edu} 12, 27 -- Subject: [Microscopy] RE: EM & LM Digital database 12, 27 -- In-reply-to: {200703150430.l2F4UvOb008596-at-ns.microscopy.com} 12, 27 -- To: mark.talbot-at-csiro.au, Microscopy Listserver {microscopy-at-microscopy.com} 12, 27 -- Message-id: {p06240800c21f3890b1ef-at-[144.92.238.207]} 12, 27 -- MIME-version: 1.0 12, 27 -- Content-type: text/plain; charset=us-ascii; format=flowed 12, 27 -- Content-transfer-encoding: 7BIT 12, 27 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=144.92.238.207 12, 27 -- X-Spam-PmxInfo: Server=avs-5, Version=5.2.1.279297, 12, 27 -- Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.3.15.104934, 12, 27 -- SenderIP=144.92.238.207 12, 27 -- References: {200703150430.l2F4UvOb008596-at-ns.microscopy.com} ==============================End of - Headers==============================
==============================Original Headers============================== 29, 24 -- From keith.morris-at-ucl.ac.uk Fri Mar 16 06:27:10 2007 29, 24 -- Received: from smtp3.global.net.uk (smtp3.global.net.uk [80.189.92.91]) 29, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2GBR9SF031047 29, 24 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 06:27:09 -0500 29, 24 -- Received: from 46.178.adsl.brightview.com ([80.189.178.46] helo=loungepc) 29, 24 -- by smtp3.global.net.uk with esmtp (Exim 4.66 (FreeBSD)) 29, 24 -- (envelope-from {keith.morris-at-ucl.ac.uk} ) 29, 24 -- id 1HSAaS-000DEs-2Z 29, 24 -- for microscopy-at-microscopy.com; Fri, 16 Mar 2007 11:27:08 +0000 29, 24 -- From: "Keith Morris" {keith.morris-at-ucl.ac.uk} 29, 24 -- To: {microscopy-at-microscopy.com} 29, 24 -- References: {200703151805.l2FI5Ehk009865-at-ns.microscopy.com} 29, 24 -- Subject: RE: [Microscopy] RE: EM & LM Digital database 29, 24 -- Date: Fri, 16 Mar 2007 11:27:14 -0000 29, 24 -- Message-ID: {000301c767be$0c085bb0$0201a8c0-at-loungepc} 29, 24 -- MIME-Version: 1.0 29, 24 -- Content-Type: text/plain; 29, 24 -- charset="us-ascii" 29, 24 -- Content-Transfer-Encoding: 7bit 29, 24 -- X-Mailer: Microsoft Office Outlook 11 29, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 29, 24 -- Thread-Index: AcdnLIFezV/xBxWMSoOqaTcKUbAXGgAj0rwg 29, 24 -- In-Reply-To: {200703151805.l2FI5Ehk009865-at-ns.microscopy.com} 29, 24 -- Authenticated-Sender: ==============================End of - Headers==============================
The Open Microscopy Environment (OME) System is being used by labs for their research, a few examples are here: http://www.openmicroscopy.org/use/ OME is being actively developed and while it has several components such as Bio-Formats (http://www.loci.wisc.edu/ome/formats.html) that are being actively used and deployed by non-developer microscopists much of the current OME system is geared towards labs with developer experience. There are active efforts to improve functionality, user installation and performance. One example is the OMERO project which I have pasted below from a recent announcement to the confocal list. For a lab with no development experience looking for a off the shelf solution with the functionality that the original poster requested, the OME system is not currently the answer but will be in the future as it continues to develop (I should note that the focus is now on optical microscopy but we hope to eventually target electron microscopy data as well). The OME Group gives frequent presentations to update the community on its progress and in fact there will be such a update meeting in Paris on March 30th. I have pasted a announcement about this below. We also have a update meeting about OME in the US at the yearly American Society for Cell Biology Meeting. There are active OME listserves that anyone can join at the www.openmicroscopy.org webpage which also has frequent updates and summaries of meetings. For labs with development interest or companies interested in helping we encourage and need additional OME developer collaborators. Please feel free to contact myself or Jason Swedlow with any questions or comments.
best, kevin
********** The OME project is pleased to announce the release of OMERO3.0- Beta1. OMERO is a Java Enterprise port of the data management and visualization functions of the perl-based OME Server. The OMERO Server ships with a JBOSS Application Server, PostgreSQL RDMS, and uses three client applications: OMERO.insight, OMERO.importer, and OMERO.admin.
All OMERO resources are available at our newly designed software download page:
http://openmicroscopy.org/software/
For new initiates, User Guides for OMERO client applications are available at our MilestoneDownloads page:
OMERO installation on OS X is all by Mac packages and should be automatic. There are manual install instructions for Linux and OS X geeks.
Please let us know any problems, wishes etc.
**************
Some background and resources:
OMERO3 is a Java EE (formerly J2EE) application designed for the JBoss application server. OMERO3 runs under Linux and OS X and uses Hibernate for mapping to a PostgreSQL database (support for MySQL and Oracle are in progress, but have been demonstrated; contact us if interested). OMERO3 requires Java1.5. Our testing has all occurred on Pentium IV or AMD 250-series systems, with 1-2 GB RAM and a Gbit network connection. The OMERO3 docs page is at:
OMERO.insight is our cross-platform Java client that uses an OMERO3 server. OMERO.insight is our first client; OMERO3 is designed to support a number of different clients and client platforms (we are considering our long-term strategies for client environments). OMERO.insight seems to run well under Linux (so far, tested on RHE3), Windows XP-SP2, and OS X; the package requires Java1.5. We have used OMERO.insight on laptops with G4, Intel for Mac, and Intel P4 processors, usually with 0.5 - 1 GB RAM and 100Mbit or Gbit NICs.
OMERO.importer is our data importer. This is a Java client (requires Java1.5), that currently imports data from a client filesystem into an OMERO3 server. For import of external file formats, the OMERO project is using BioFormats- see http://loci.wisc.edu/ome/ formats.html. Currently, OMEROImport supports two file formats-- APLLC DV and MetaMorph's STK. We are of course working to include OMERO.importer as a component of OMERO.insight, as well as supporting server-side import. A major goal is the extension of proprietary file format support in OMERO.importer to include all files supported in Bio-Formats. We, along with our colleagues at LOCI, are working hard to expand the range of proprietary file formats supported in BioFormats. Stay tuned!
What does OMERO do? Mostly, image visualization and image data management. There is not yet support for any management of image analysis results and data-- this continues to be one of the hallmarks of the released OME 2.x server (OME2.6.0 is now available at http:// cvs.openmicroscopy.org.uk; we use this system in the lab for large- scale image analysis). The OMERO3-Beta1 package provides the ability to organize image data into Projects and Datasets and also user- defined CategoryGroups and Categories (see http://openmicroscopy.org/ getting-started/manual_classification.html). In addition, our server-based Rendering Engine provides image visualization functions in a remote client environment. Our testing suggests that this that is a very useful tool for visualising image data in a client-server environment.
Note that the OME and OMERO Servers are parallel, complementary projects. They are currently focussing on different function sets-- see http://www.openmicroscopy.org/software/why2servers.html for more info.
We have recognized the importance of getting feedback on the design, development, and documentation of OMERO and its clients. OMERO clients include an automatic bug logging system-- using this helps us to help you. As the above web pages suggest, we target our work around a series of milestones, and try to produce packages for testing these milestones. These should not be seen as finished, released software, but they are tested and (largely) work as promised. They are beta and should demonstrate what OMERO can achieve.
Thanks again for your interest and support.
Cheers,
Jason Swedlow Open Microscopy Environment: http://openmicroscopy.org
******** The OME project is pleased to announce the next OME European Users Meeting, to be held at the Le Meditel Club in Paris, in association with Institut Pasteur, March 29/30, 2007. Spencer Shorte, the Director of the Plateforme d'Imagerie Dynamique at the Institut Pasteur has kindly agreed to host the meeting.
A draft meeting programme is at the OME website (http:// openmicroscopy.org/latest/euro2007-03.html). We will start the morning of March 29 with presentations of newly released software, including the new OME Server2.6.0, the new Java-based OMERO Server and its clients (see http://openmicroscopy.org/software/), the OME file formats (http://www.loci.wisc.edu/ome/formats.html & http:// www.loci.wisc.edu/ome/ome-tiff-spec.html), format conversion library (Bio-Formats; http://loci.wisc.edu/ome/formats.html) and our efforts to drive usability of our tools (http://www.usableimage.com). The current programme also includes presentations from Anne Carpenter, from the Broad Institute on CellProfiler an CellVisualizer (http:// www.cellprofiler.org/), Curtis Rueden from LOCI, Univ of Wisconsin, Madison (http://www.loci.wisc.edu) and Patrick Courtney (PerkinElmer LifeSciences).
March 30 will focus on defining goals for critical functionality for OME and other tools, using breakout groups led by experts in data models, file formats, usability testing and software development. We hope to generate a broad roadmap that will define this community's focus for the next year.
Currently, we have about 30 attendees signed up form US, UK, France, Switzerland from both academia and commercial institutions.
If you are interested in attending , please send your name, institution, and contact details to:
Mme Christiane Pacaud (the Imagopole Administrative Assistant), chrispac-at-pasteur.fr
It will help us if you tell us when you will be arriving and leaving Paris (to organise airport/train transport, if possible-- no guarantees, but we will try).
Any requests for presentations or other programme issues can be addressed to myself and Spencer.
We regret we have no funds to pay for travel or accommodation of participants, but we will cover coffee and lunch during the meeting.
See you in Paris!
Cheers,
Jason ************
----- Original Message ----- X-from: keith.morris-at-ucl.ac.uk
Hello Mark & Paul We've just gone through this exercise for the Biotron (here at University of Western Ontario). Our requirements were for a robust internet accessible data base that could handle and correlate data in many different formats (confocal, LM, TEM, SEM, flow cytometery, GS/MS, spectral data, spreadsheet output from analysis systems and point data such as temperatures, humidity, etc.) we were also looking for a system that could handle a large ( 10's - 100's of TB) data base information and also allow sophisticated queries and provide for video, audio and on-line chats all with a (reasonably) user friendly interface. We looked at and evaluated a large selection of 'off the shelf' products and a panoply of custom programmed systems. We chose a product marketed by Hitachi High Technologies - the Quartz/PCI Wide Area Microscopy suite (no financial interests in this group - but we are satisfied with both the product and our relation with Hitachi). Feel free to contact me off list for more detailed discussions.
Rick Richard Harris Manager - Imaging and Data Systems The Biotron Climate Change Research Facility The University of Western Ontario London ON CA www.biotron.uwo.ca
-----Original Message----- X-from: mark.talbot-at-csiro.au [mailto:mark.talbot-at-csiro.au] Sent: Thursday, March 15, 2007 12:30 AM To: rjharris-at-uwo.ca
Hi Paul,
I have exactly the same question. I am trying to find a database that will coordinate the cataloguing of images from SEM, confocal and light microscopes from a large number of regular and temporary users. The software not only needs to catalogue the images but also accommodate updateable links to electronic 'notebooks' which describe experimental details and results (which can be accessed over a network and edited by people associated with the project). It is also preferable to database such things as linked PDFs (relevant literature) and data files such as Excel spreadsheets.
I have so far not managed to find one single program that can do all of this! There's a great database program called Pax-It, but this is a little bit user-unfriendly (one important stipulation is that the program needs to be user-friendly since it would be used by a large mix of people with a gradation of computer literacy) and so far I can't see any ability to database together with electronic notebooks. But I may be asking too much? If anyone could help with this, I would be very happy to hear from you (and Paul as well).
Thanks,
Mark
Dr. Mark Talbot CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia mark.talbot-at-csiro.au
ph. 61 (0)2-6246 5256
fax. 61 (0)2-6246 5334
-----Original Message----- X-from: PWebster-at-hei.org [mailto:PWebster-at-hei.org] Sent: Thursday, 8 March 2007 4:42 AM To: Talbot, Mark (PI, Black Mountain)
Hi,
We need a way to catalog stored images (EM & LM) together with experimental data (text) for access by in-house researchers as well as off-site users.
Does anyone know of any commercial products available that would fit our needs?
We have someone here who would like to try to develop this but it will be a waste of time and money if something is already available.
Regards,
Paul.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
==============================Original Headers============================== 10, 18 -- From PWebster-at-hei.org Wed Mar 7 11:37:08 2007 10, 18 -- Received: from hi0sml1.hei.org (House-Ear-Institute-1114861.cust-rtr.pacbell.net [71.136.31.242]) 10, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l27Hb7C7009704 10, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Mar 2007 11:37:08 -0600 10, 18 -- Received: from 10.10.42.96 ([10.10.42.96]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 10, 18 -- Wed, 7 Mar 2007 17:37:07 +0000 10, 18 -- User-Agent: Microsoft-Entourage/11.3.3.061214 10, 18 -- Date: Wed, 07 Mar 2007 09:37:06 -0800 10, 18 -- Subject: EM & LM Digital database 10, 18 -- From: "Webster, Paul" {PWebster-at-hei.org} 10, 18 -- To: {Microscopy-at-Microscopy.Com} 10, 18 -- Message-ID: {C2143842.FBF9%PWebster-at-hei.org} 10, 18 -- Thread-Topic: EM & LM Digital database 10, 18 -- Thread-Index: Acdg3zlheCaVzszSEduDkgANk7Zh7g== 10, 18 -- Mime-version: 1.0 10, 18 -- Content-type: text/plain; 10, 18 -- charset="US-ASCII" 10, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
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==============================Original Headers============================== 36, 26 -- From rjharris-at-uwo.ca Fri Mar 16 10:26:28 2007 36, 26 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146]) 36, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2GFQRSe027413 36, 26 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 16 Mar 2007 10:26:28 -0500 36, 26 -- Received: from zeppo.mail.uwo.pri (whelan.mail.uwo.pri [172.29.32.40]) 36, 26 -- by zeppo.mail.uwo.pri 36, 26 -- (Sun Java System Messaging Server 6.2-8.04 (built Feb 28 2007)) 36, 26 -- with ESMTP id {0JF0000Y45K2DND0-at-zeppo.mail.uwo.pri} for 36, 26 -- Microscopy-at-MSA.Microscopy.Com; Fri, 16 Mar 2007 11:26:26 -0400 (EDT) 36, 26 -- Received: from RICKNOTEBOOK ([129.100.68.106]) 36, 26 -- by zeppo.mail.uwo.pri (Sun Java System Messaging Server 6.2-8.04 (built Feb 28 36, 26 -- 2007)) with ESMTPS id {0JF0002OA5K2P0D0-at-zeppo.mail.uwo.pri} for 36, 26 -- Microscopy-at-MSA.Microscopy.Com; Fri, 16 Mar 2007 11:26:26 -0400 (EDT) 36, 26 -- Date: Fri, 16 Mar 2007 11:26:26 -0400 36, 26 -- From: Richard Harris {rjharris-at-uwo.ca} 36, 26 -- Subject: RE: [Microscopy] RE: EM & LM Digital database 36, 26 -- In-reply-to: {200703150430.l2F4UR8r007222-at-ns.microscopy.com} 36, 26 -- To: mark.talbot-at-csiro.au 36, 26 -- Cc: MSA Listserver {Microscopy-at-MSA.Microscopy.Com} 36, 26 -- Message-id: {0JF0002OB5K2P0D0-at-zeppo.mail.uwo.pri} 36, 26 -- MIME-version: 1.0 36, 26 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 36, 26 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 36, 26 -- Content-type: text/plain; charset=us-ascii 36, 26 -- Content-transfer-encoding: 7BIT 36, 26 -- Thread-index: Acdmuqh8kpDxEa/FQemo6D+ooAH3bQBHteFA ==============================End of - Headers==============================
We have a shorted biomedical leadwire. We've used test equipment to verify the short. What we would like to do is to visually identify the location of the short - in the SEM. The leadwire consists of 4 ea ~1mm insulated wires that are then sheathed in insulation. We can sacrifice the outer insulation but need to retain insulation on the inner wires. I've imagined that if we power the cables inside the SEM, we might see current contrast that would divert from one conductor to another at the short.
Is this experiment as easy as;
building a vacuum feedthrough, connecting a external bench power supply wiring the cable to the feedthrough inside the SEM turn it all on and... WAH LAH! current contrast???
Probably not that easy, so what am I missing?
Owen Mills Michigan TEch University
==============================Original Headers============================== 6, 31 -- From opmills-at-mtu.edu Fri Mar 16 13:16:20 2007 6, 31 -- Received: from node8.mtu.edu (node8.mtu.edu [141.219.69.8]) 6, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2GIGKkL010117 6, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 13:16:20 -0500 6, 31 -- Received: from node6.edge.dcsint.mtu.edu (node6.mtu.edu [141.219.69.6]) 6, 31 -- by node8.mtu.edu (8.13.1/8.13.1) with ESMTP id l2GIGKJB020251 6, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 14:16:20 -0400 6, 31 -- Received: from node34.edge.dcsint.mtu.edu (node34.mtu.edu [141.219.69.34]) 6, 31 -- by node6.edge.dcsint.mtu.edu (8.12.11.20060308/8.12.8) with ESMTP id l2GIGJjb012892 6, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 14:16:19 -0400 6, 31 -- Received: from mail.mtu.edu (campus0.mtu.edu [141.219.70.8]) 6, 31 -- by node34.edge.dcsint.mtu.edu (8.13.8/8.13.8) with ESMTP id l2GIGJGP018320 6, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 14:16:19 -0400 6, 31 -- (envelope-from opmills-at-mtu.edu) 6, 31 -- Received: from node6.edge.dcsint.mtu.edu (node6.mtu.edu [141.219.69.6]) 6, 31 -- by mail.mtu.edu (8.13.6+Sun/8.11.6) with ESMTP id l2GIGJ21000702 6, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 14:16:19 -0400 (EDT) 6, 31 -- Received: from [141.219.192.45] (opmills.eecn.sabo.mtu.edu [141.219.192.45]) 6, 31 -- by node6.edge.dcsint.mtu.edu (8.12.11.20060308/8.12.3/auth_ssl.mc v1.0) with ESMTP id l2GIGJpk012886 6, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 14:16:19 -0400 6, 31 -- Mime-Version: 1.0 (Apple Message framework v752.3) 6, 31 -- Content-Transfer-Encoding: 7bit 6, 31 -- Message-Id: {3742B1C4-988E-47E2-A3F5-696F1263F1CE-at-mtu.edu} 6, 31 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 31 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 31 -- From: "Owen P. Mills" {opmills-at-mtu.edu} 6, 31 -- Subject: shorted leadwires - current contrast? 6, 31 -- Date: Fri, 16 Mar 2007 14:16:17 -0400 6, 31 -- X-Mailer: Apple Mail (2.752.3) 6, 31 -- X-PMX-Version: 5.3.1.294258, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.3.16.110433 6, 31 -- X-PerlMx-Spam: Gauge=IIIIIII, Probability=7%, Report='__CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
Can you describe your application a little further? If you need something that will measure at the macromolecular level, try AFM. If on a larger scale, both interferometry and confocal microscopy may be good choices.
Hope this is helpful, Barbara Foster, President
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
MME is now scheduling customized, on-site courses through July. Call us today for details.
P. S. Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 11:31 AM 3/16/2007, stmccart-at-vt.edu wrote:
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Thinking about your problem, I don't think that SEM is the answer. When you run current through the wires, it will generate heat, not electrons. If your SEM has CL, then it probably would work.
I think the only practical (but potentially destructive) method is to pass enough current to cause the shorted area to heat up and burn. Or, run as much current as you can such that the wires do not burn and use an IR laser temperature gun and scan the wires for a drop in temperature. Just at this point is past the short.
The amount of current needed is of course dependent on the resistance of the input end of the wires.
gary g.
At 10:19 AM 3/16/2007, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Fri Mar 16 17:04:46 2007 11, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2GM4jZ7004675 11, 20 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 17:04:46 -0500 11, 20 -- Message-Id: {200703162204.l2GM4jZ7004675-at-ns.microscopy.com} 11, 20 -- Received: (qmail 13567 invoked from network); 16 Mar 2007 15:04:45 -0700 11, 20 -- Received: by simscan 1.1.0 ppid: 13564, pid: 13565, t: 0.0947s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 20 -- by qsmtp1 with SMTP; 16 Mar 2007 15:04:45 -0700 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Fri, 16 Mar 2007 15:04:40 -0800 11, 20 -- To: opmills-at-mtu.edu 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] shorted leadwires - current contrast? 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200703161819.l2GIJQOJ013114-at-ns.microscopy.com} 11, 20 -- References: {200703161819.l2GIJQOJ013114-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-449A46B0 ==============================End of - Headers==============================
Real-time x-ray imaging will give you the answer you need. Many universities and commercial failure analysis labs have the capability.
--John
John Chandler Colorado School of Mines jpchandl-at-mines.edu 303-384-2203
-----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: Friday, March 16, 2007 4:11 PM To: jpchandl-at-mines.edu
Thinking about your problem, I don't think that SEM is the answer. When you run current through the wires, it will generate heat, not electrons. If your SEM has CL, then it probably would work.
I think the only practical (but potentially destructive) method is to pass enough current to cause the shorted area to heat up and burn. Or, run as much current as you can such that the wires do not burn and use an IR laser temperature gun and scan the wires for a drop in temperature. Just at this point is past the short.
The amount of current needed is of course dependent on the resistance of the input end of the wires.
gary g.
At 10:19 AM 3/16/2007, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Fri Mar 16 17:04:46 2007 11, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2GM4jZ7004675 11, 20 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 17:04:46 -0500 11, 20 -- Message-Id: {200703162204.l2GM4jZ7004675-at-ns.microscopy.com} 11, 20 -- Received: (qmail 13567 invoked from network); 16 Mar 2007 15:04:45 -0700 11, 20 -- Received: by simscan 1.1.0 ppid: 13564, pid: 13565, t: 0.0947s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 20 -- by qsmtp1 with SMTP; 16 Mar 2007 15:04:45 -0700 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Fri, 16 Mar 2007 15:04:40 -0800 11, 20 -- To: opmills-at-mtu.edu 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] shorted leadwires - current contrast? 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200703161819.l2GIJQOJ013114-at-ns.microscopy.com} 11, 20 -- References: {200703161819.l2GIJQOJ013114-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-449A46B0 ==============================End of - Headers==============================
==============================Original Headers============================== 21, 21 -- From jpchandl-at-mines.edu Fri Mar 16 17:18:38 2007 21, 21 -- Received: from inception.Mines.EDU (inception.Mines.EDU [138.67.130.4]) 21, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2GMIcpY016201 21, 21 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 17:18:38 -0500 21, 21 -- Received: from emlab1 (emlab1.Mines.EDU [138.67.28.173]) 21, 21 -- by inception.Mines.EDU (8.13.1/8.13.1) with ESMTP id l2GMIU7V010806; 21, 21 -- Fri, 16 Mar 2007 16:18:30 -0600 21, 21 -- From: "John Chandler" {jpchandl-at-mines.edu} 21, 21 -- To: {gary-at-gaugler.com} , {microscopy-at-microscopy.com} 21, 21 -- References: {200703162210.l2GMAXcX014697-at-ns.microscopy.com} 21, 21 -- Subject: RE: [Microscopy] Re: shorted leadwires - current contrast? 21, 21 -- Date: Fri, 16 Mar 2007 16:18:30 -0600 21, 21 -- Message-ID: {001601c76819$072efee0$ad1c438a-at-mines.edu} 21, 21 -- MIME-Version: 1.0 21, 21 -- Content-Type: text/plain; 21, 21 -- charset="us-ascii" 21, 21 -- Content-Transfer-Encoding: 7bit 21, 21 -- X-Mailer: Microsoft Office Outlook 11 21, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 21, 21 -- Thread-Index: AcdoF/BkCzweFlYPQR2yjawxZbdn1AAAIYwQ 21, 21 -- In-Reply-To: {200703162210.l2GMAXcX014697-at-ns.microscopy.com} ==============================End of - Headers==============================
We have a new SEM on the way to us, it's a JEOL JSM-840. All I have at this point are a couple of pictures, and I am a little flummoxed about the accessories that are added on this instrument. I recognize the secondary electron detector, and I believe that there is a backscatter detector on it, but I'm not sure what the other accessories are. Also, the photos that I have seen of the basic 840s don't include a box sitting on top of the column, but this one has it. It's a silver-ish box with black sides, and is barely visible in one of the photos, but it is there, at a slight angle. (Which leads me to the next question- how should it be secured for shipping?)
The whole microscope is being packed by people who don't necessarily know how to package it. Do any of you have specific suggestions of what they could do to prevent damage in shipping?
Here is the URL to the photos of the microscope (Hosted on my personal page which has not been updated in a long time...) http://www.jkraft.net/JEOL/
Thanks,
Justin A. Kraft Director Center for Inquiry-Based Science Education
==============================Original Headers============================== 6, 26 -- From kraftpiano-at-gmail.com Fri Mar 16 17:40:22 2007 6, 26 -- Received: from ik-out-1112.google.com (ik-out-1112.google.com [66.249.90.177]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2GMeL9w006622 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 17:40:22 -0500 6, 26 -- Received: by ik-out-1112.google.com with SMTP id b32so541347ika 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 15:40:20 -0700 (PDT) 6, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 6, 26 -- d=gmail.com; s=beta; 6, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 6, 26 -- b=s1DejygYHazI0gOXcGdlH3aFhc31xI8o6mt+j5GOcTzrxtJPW9PLWzsfWcdgsZQjbUfGwio8NUweB8pHDxwgatI0zP5kv+TIfnL1G2A6U75mJ90Wu2/xog0gZFV7g5sBV3vJe5jiayVV4dGSkp74tvq2VQyDtZdd3Icvrv93WZs= 6, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 26 -- d=gmail.com; s=beta; 6, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 6, 26 -- b=pR3ppcp1LBgkdhNL2mxb8fxkll/KXvwAFHADQW/u6FINK4J15EsY55ex1+B2+g0JNvff5T3gaaYDWFX6PzBDM720L+slCXtxDVdzFMBAPEocfGL+xIvvPiSQg1ACUQ8+ujyW+Q/J0di/cxKyHEXk7+jE105VLOBzCHBFkml2E+0= 6, 26 -- Received: by 10.114.171.1 with SMTP id t1mr918673wae.1174084819773; 6, 26 -- Fri, 16 Mar 2007 15:40:19 -0700 (PDT) 6, 26 -- Received: by 10.114.79.12 with HTTP; Fri, 16 Mar 2007 15:40:19 -0700 (PDT) 6, 26 -- Message-ID: {25e2b0d20703161540k140001d5k6170427b9d645d6b-at-mail.gmail.com} 6, 26 -- Date: Fri, 16 Mar 2007 18:40:19 -0400 6, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 6, 26 -- To: microscopy-at-microscopy.com 6, 26 -- Subject: New SEM donated to our lab- Can you identify the accessory? 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 26 -- Content-Transfer-Encoding: 7bit 6, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
Your 840 has been configured with microprobe capability. I think that this is called the 840A configuration, but you'll have to check with that. You have a wavelength dispersive spectrometer, an optical microscope for setting the focus working distance for the spectrometer, and I believe that there is a probe current meter there, but I can't make it out well.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Friday, March 16, 2007 2:44 PM To: Walck-at-SouthBayTech.com
Hello all,
We have a new SEM on the way to us, it's a JEOL JSM-840. All I have at this point are a couple of pictures, and I am a little flummoxed about the accessories that are added on this instrument. I recognize the secondary electron detector, and I believe that there is a backscatter detector on it, but I'm not sure what the other accessories are. Also, the photos that I have seen of the basic 840s don't include a box sitting on top of the column, but this one has it. It's a silver-ish box with black sides, and is barely visible in one of the photos, but it is there, at a slight angle. (Which leads me to the next question- how should it be secured for shipping?)
The whole microscope is being packed by people who don't necessarily know how to package it. Do any of you have specific suggestions of what they could do to prevent damage in shipping?
Here is the URL to the photos of the microscope (Hosted on my personal page which has not been updated in a long time...) http://www.jkraft.net/JEOL/
Thanks,
Justin A. Kraft Director Center for Inquiry-Based Science Education
==============================Original Headers============================== 6, 26 -- From kraftpiano-at-gmail.com Fri Mar 16 17:40:22 2007 6, 26 -- Received: from ik-out-1112.google.com (ik-out-1112.google.com [66.249.90.177]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2GMeL9w006622 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 17:40:22 -0500 6, 26 -- Received: by ik-out-1112.google.com with SMTP id b32so541347ika 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 15:40:20 -0700 (PDT) 6, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 6, 26 -- d=gmail.com; s=beta; 6, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime -version:content-type:content-transfer-encoding:content-disposition; 6, 26 -- b=s1DejygYHazI0gOXcGdlH3aFhc31xI8o6mt+j5GOcTzrxtJPW9PLWzsfWcdgsZQjbUfGwio8NU weB8pHDxwgatI0zP5kv+TIfnL1G2A6U75mJ90Wu2/xog0gZFV7g5sBV3vJe5jiayVV4dGSkp74tv q2VQyDtZdd3Icvrv93WZs= 6, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 26 -- d=gmail.com; s=beta; 6, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content -transfer-encoding:content-disposition; 6, 26 -- b=pR3ppcp1LBgkdhNL2mxb8fxkll/KXvwAFHADQW/u6FINK4J15EsY55ex1+B2+g0JNvff5T3gaa YDWFX6PzBDM720L+slCXtxDVdzFMBAPEocfGL+xIvvPiSQg1ACUQ8+ujyW+Q/J0di/cxKyHEXk7+ jE105VLOBzCHBFkml2E+0= 6, 26 -- Received: by 10.114.171.1 with SMTP id t1mr918673wae.1174084819773; 6, 26 -- Fri, 16 Mar 2007 15:40:19 -0700 (PDT) 6, 26 -- Received: by 10.114.79.12 with HTTP; Fri, 16 Mar 2007 15:40:19 -0700 (PDT) 6, 26 -- Message-ID: {25e2b0d20703161540k140001d5k6170427b9d645d6b-at-mail.gmail.com} 6, 26 -- Date: Fri, 16 Mar 2007 18:40:19 -0400 6, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 6, 26 -- To: microscopy-at-microscopy.com 6, 26 --
In the SEM, the secondary electron signal is roughly proportional to the potential the specimen surface.
Jim P.
Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU 3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver Canada Info: http://www.3dcourse.ubc.ca/ Applications due by March 20, 2007 "If it ain't diffraction, it must be statistics." Anon.
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-- ********************************************** Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU 3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver Canada Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2007 "If it ain't diffraction, it must be statistics." Anon.
==============================Original Headers============================== 7, 27 -- From jbpawley-at-wisc.edu Fri Mar 16 19:06:49 2007 7, 27 -- Received: from agogare.doit.wisc.edu (agogare.doit.wisc.edu [144.92.197.211]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2H06mhx030314 7, 27 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 19:06:49 -0500 7, 27 -- Received: from avs-daemon.smtpauth2.wiscmail.wisc.edu by 7, 27 -- smtpauth2.wiscmail.wisc.edu 7, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 7, 27 -- id {0JF000E01TNBPH00-at-smtpauth2.wiscmail.wisc.edu} for 7, 27 -- microscopy-at-microscopy.com; Fri, 16 Mar 2007 19:06:47 -0500 (CDT) 7, 27 -- Received: from [144.92.238.207] by smtpauth2.wiscmail.wisc.edu 7, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 7, 27 -- with ESMTPSA id {0JF0007UZTNAHH40-at-smtpauth2.wiscmail.wisc.edu} for 7, 27 -- microscopy-at-microscopy.com; Fri, 16 Mar 2007 19:06:47 -0500 (CDT) 7, 27 -- Date: Fri, 16 Mar 2007 19:06:45 -0500 7, 27 -- From: James Pawley {jbpawley-at-wisc.edu} 7, 27 -- Subject: [Microscopy] Re: shorted leadwires - current contrast? 7, 27 -- In-reply-to: {200703162210.l2GMAtvC015439-at-ns.microscopy.com} 7, 27 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 7, 27 -- Message-id: {p06240806c220e0d75262-at-[144.92.238.207]} 7, 27 -- MIME-version: 1.0 7, 27 -- Content-type: text/plain; charset=us-ascii; format=flowed 7, 27 -- Content-transfer-encoding: 7BIT 7, 27 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=144.92.238.207 7, 27 -- X-Spam-PmxInfo: Server=avs-12, Version=5.3.0.289146, 7, 27 -- Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.3.16.165433, 7, 27 -- SenderIP=144.92.238.207 7, 27 -- References: {200703162210.l2GMAtvC015439-at-ns.microscopy.com} ==============================End of - Headers==============================
Justin, I believe what you have is a JXA-840 (microprobe) with 2 wavelength spectrometers and a light microscope for establishing the proper WD for using those spectrometers. The Box on top is an ion pump so that the system can be used with a LaB6 cathode, if you choose (and have received the correct wehnelt cap).
Whether it is an 840 or 840A could be told by a look at the electronics console, but you don't have any pictures of that or the electronics rack that should be present for the spectometers. I do see the power supply console in a couple of pictures. Does it include the rotary pump and possibly compressor?
As far as prepping for shipping, the first thing that jumps out at me is that the table isn't bolted down! At a minimum you need to get 4 M16x2.00x40mm bolts and 16mm washers to secure the table. You also need to remove the magnet from the ion pump. Actually, most of the column should be removed and packed separately unless you're planning to move it only a few miles at very low speed. I also suspect that the WDS units and light microscope should be removed. All 3 are complex and fragile.
Is it diffusion pumped or turbo pumped? If it's turbo pumped, the pump must be removed before shipping. Also the high voltage oil tank in the electronics console must be removed and the circuit board on its side protected from damage.
This is going to the W. Palm Beach area of Florida. Where is it currently located? I would highly recommend sending someone to pack it properly. How is it going to be shipped? If it's going by truck, it must be "padded van" unless you're going to have a lot more of it disassembled, crated and put on pallets.
With more info, I might be of more help.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Friday, March 16, 2007 6:43 PM To: kenconverse-at-qualityimages.biz
Hello all,
We have a new SEM on the way to us, it's a JEOL JSM-840. All I have at this point are a couple of pictures, and I am a little flummoxed about the accessories that are added on this instrument. I recognize the secondary electron detector, and I believe that there is a backscatter detector on it, but I'm not sure what the other accessories are. Also, the photos that I have seen of the basic 840s don't include a box sitting on top of the column, but this one has it. It's a silver-ish box with black sides, and is barely visible in one of the photos, but it is there, at a slight angle. (Which leads me to the next question- how should it be secured for shipping?)
The whole microscope is being packed by people who don't necessarily know how to package it. Do any of you have specific suggestions of what they could do to prevent damage in shipping?
Here is the URL to the photos of the microscope (Hosted on my personal page which has not been updated in a long time...) http://www.jkraft.net/JEOL/
Thanks,
Justin A. Kraft Director Center for Inquiry-Based Science Education
==============================Original Headers============================== 6, 26 -- From kraftpiano-at-gmail.com Fri Mar 16 17:40:22 2007 6, 26 -- Received: from ik-out-1112.google.com (ik-out-1112.google.com [66.249.90.177]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2GMeL9w006622 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 17:40:22 -0500 6, 26 -- Received: by ik-out-1112.google.com with SMTP id b32so541347ika 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 15:40:20 -0700 (PDT) 6, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 6, 26 -- d=gmail.com; s=beta; 6, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime -version:content-type:content-transfer-encoding:content-disposition; 6, 26 -- b=s1DejygYHazI0gOXcGdlH3aFhc31xI8o6mt+j5GOcTzrxtJPW9PLWzsfWcdgsZQjbUfGwio8NU weB8pHDxwgatI0zP5kv+TIfnL1G2A6U75mJ90Wu2/xog0gZFV7g5sBV3vJe5jiayVV4dGSkp74tv q2VQyDtZdd3Icvrv93WZs= 6, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 26 -- d=gmail.com; s=beta; 6, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content -transfer-encoding:content-disposition; 6, 26 -- b=pR3ppcp1LBgkdhNL2mxb8fxkll/KXvwAFHADQW/u6FINK4J15EsY55ex1+B2+g0JNvff5T3gaa YDWFX6PzBDM720L+slCXtxDVdzFMBAPEocfGL+xIvvPiSQg1ACUQ8+ujyW+Q/J0di/cxKyHEXk7+ jE105VLOBzCHBFkml2E+0= 6, 26 -- Received: by 10.114.171.1 with SMTP id t1mr918673wae.1174084819773; 6, 26 -- Fri, 16 Mar 2007 15:40:19 -0700 (PDT) 6, 26 -- Received: by 10.114.79.12 with HTTP; Fri, 16 Mar 2007 15:40:19 -0700 (PDT) 6, 26 -- Message-ID: {25e2b0d20703161540k140001d5k6170427b9d645d6b-at-mail.gmail.com} 6, 26 -- Date: Fri, 16 Mar 2007 18:40:19 -0400 6, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 6, 26 -- To: microscopy-at-microscopy.com 6, 26 -- Subject: New SEM donated to our lab- Can you identify the accessory? 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 26 -- Content-Transfer-Encoding: 7bit 6, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
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==============================Original Headers============================== 28, 29 -- From kenconverse-at-qualityimages.biz Sat Mar 17 11:36:36 2007 28, 29 -- Received: from dpmailmta02.doteasy.com (dpmailmta02.doteasy.com [65.61.209.90]) 28, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2HGaaU1026076 28, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sat, 17 Mar 2007 11:36:36 -0500 28, 29 -- Received: from qualityimages.biz (unverified [192.168.101.16]) 28, 29 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id 67056218-1814644 28, 29 -- for multiple; Sat, 17 Mar 2007 09:46:58 -0800 28, 29 -- Received: from Ken [76.179.237.214] by qualityimages.biz with ESMTP 28, 29 -- (SMTPD32-8.05) id A90E1B650056; Sat, 17 Mar 2007 09:36:30 -0700 28, 29 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 28, 29 -- To: {kraftpiano-at-gmail.com} , "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 28, 29 -- Subject: RE: [Microscopy] New SEM donated to our lab- Can you identify the accessory? 28, 29 -- Date: Sat, 17 Mar 2007 12:36:18 -0400 28, 29 -- Message-ID: {004001c768b2$654b4e90$6401a8c0-at-Ken} 28, 29 -- MIME-Version: 1.0 28, 29 -- Content-Type: text/plain; 28, 29 -- charset="us-ascii" 28, 29 -- X-Priority: 3 (Normal) 28, 29 -- X-MSMail-Priority: Normal 28, 29 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 28, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 28, 29 -- Thread-Index: AcdoHH2p3h+gQ0uxT2OvjUqyLu6bXwAkPKTg 28, 29 -- Importance: Normal 28, 29 -- In-Reply-To: {200703162243.l2GMhGZg011302-at-ns.microscopy.com} 28, 29 -- X-IMSTrailer: __IMail_7__ 28, 29 -- X-IP-stats: Incoming Last 0, First 170, in=3711794, out=0, spam=0 ip=192.168.101.16 28, 29 -- X-Originating-IP: 192.168.101.16 28, 29 -- Content-Transfer-Encoding: 8bit 28, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2HGaaU1026076 ==============================End of - Headers==============================
Hi Owen, just to expand on James Pawley's comment - yes, you can see different _potentials_ (not currents) in SEM (just see what happens when you have an insulator and it charges up). This is great for looking at open circuit problems, since you can see a contrast change where the break in the circuit lies - when you apply a few volts to one side or the other of the break. Also for seeing surface potentials due to doping in semiconductors if you can keep the surface clean enough. However if you have a short circuit everything will be at the same potential and you won't be able to see the problem. I agree with John Chandler that real time X-ray radiography is the best approach. If you still want to try SEM you could connect one end of the circuit to the specimen stage and image using the specimen current signal. However you could only do this if the insulation is thin enough to get some of the electron beam through to the wires, which is probably not possible.
-----Original Message----- X-from: opmills-at-mtu.edu [mailto:opmills-at-mtu.edu] Sent: 16 March 2007 18:18 To: Richard Beanland
All,
We have a shorted biomedical leadwire. We've used test equipment to verify the short. What we would like to do is to visually identify the location of the short - in the SEM. The leadwire consists of 4 ea ~1mm insulated wires that are then sheathed in insulation. We can sacrifice the outer insulation but need to retain insulation on the inner wires. I've imagined that if we power the cables inside the SEM, we might see current contrast that would divert from one conductor to another at the short.
Is this experiment as easy as;
building a vacuum feedthrough, connecting a external bench power supply wiring the cable to the feedthrough inside the SEM turn it all on and... WAH LAH! current contrast???
Probably not that easy, so what am I missing?
Owen Mills Michigan TEch University
==============================Original Headers============================== 6, 31 -- From opmills-at-mtu.edu Fri Mar 16 13:16:20 2007 6, 31 -- Received: from node8.mtu.edu (node8.mtu.edu [141.219.69.8]) 6, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2GIGKkL010117 6, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 13:16:20 -0500 6, 31 -- Received: from node6.edge.dcsint.mtu.edu (node6.mtu.edu [141.219.69.6]) 6, 31 -- by node8.mtu.edu (8.13.1/8.13.1) with ESMTP id l2GIGKJB020251 6, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 14:16:20 -0400 6, 31 -- Received: from node34.edge.dcsint.mtu.edu (node34.mtu.edu [141.219.69.34]) 6, 31 -- by node6.edge.dcsint.mtu.edu (8.12.11.20060308/8.12.8) with ESMTP id l2GIGJjb012892 6, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 14:16:19 -0400 6, 31 -- Received: from mail.mtu.edu (campus0.mtu.edu [141.219.70.8]) 6, 31 -- by node34.edge.dcsint.mtu.edu (8.13.8/8.13.8) with ESMTP id l2GIGJGP018320 6, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 14:16:19 -0400 6, 31 -- (envelope-from opmills-at-mtu.edu) 6, 31 -- Received: from node6.edge.dcsint.mtu.edu (node6.mtu.edu [141.219.69.6]) 6, 31 -- by mail.mtu.edu (8.13.6+Sun/8.11.6) with ESMTP id l2GIGJ21000702 6, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 14:16:19 -0400 (EDT) 6, 31 -- Received: from [141.219.192.45] (opmills.eecn.sabo.mtu.edu [141.219.192.45]) 6, 31 -- by node6.edge.dcsint.mtu.edu (8.12.11.20060308/8.12.3/auth_ssl.mc v1.0) with ESMTP id l2GIGJpk012886 6, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 14:16:19 -0400 6, 31 -- Mime-Version: 1.0 (Apple Message framework v752.3) 6, 31 -- Content-Transfer-Encoding: 7bit 6, 31 -- Message-Id: {3742B1C4-988E-47E2-A3F5-696F1263F1CE-at-mtu.edu} 6, 31 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 31 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 31 -- From: "Owen P. Mills" {opmills-at-mtu.edu} 6, 31 -- Subject: shorted leadwires - current contrast? 6, 31 -- Date: Fri, 16 Mar 2007 14:16:17 -0400 6, 31 -- X-Mailer: Apple Mail (2.752.3) 6, 31 -- X-PMX-Version: 5.3.1.294258, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.3.16.110433 6, 31 -- X-PerlMx-Spam: Gauge=IIIIIII, Probability=7%, Report='__CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
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==============================Original Headers============================== 17, 34 -- From richard.beanland-at-bookham.com Mon Mar 19 04:03:45 2007 17, 34 -- Received: from mail82.messagelabs.com (mail82.messagelabs.com [195.245.231.67]) 17, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2J93iRr027812 17, 34 -- for {microscopy-at-microscopy.com} ; Mon, 19 Mar 2007 04:03:45 -0500 17, 34 -- X-VirusChecked: Checked 17, 34 -- X-Env-Sender: richard.beanland-at-bookham.com 17, 34 -- X-Msg-Ref: server-15.tower-82.messagelabs.com!1174294990!31407709!7 17, 34 -- X-StarScan-Version: 5.5.10.7.1; banners=bookham.com,-,- 17, 34 -- X-Originating-IP: [213.249.209.179] 17, 34 -- Received: (qmail 27253 invoked from network); 19 Mar 2007 09:03:42 -0000 17, 34 -- Received: from unknown (HELO cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 17, 34 -- by server-15.tower-82.messagelabs.com with SMTP; 19 Mar 2007 09:03:42 -0000 17, 34 -- Received: from cas-smx-02.BOOKHAM.ENTERPRISE.PRI ([10.4.0.223]) by cas-smx-brdg-02.BOOKHAM.ENTERPRISE.PRI with Microsoft SMTPSVC(6.0.3790.1830); 17, 34 -- Mon, 19 Mar 2007 09:03:18 +0000 17, 34 -- Content-class: urn:content-classes:message 17, 34 -- MIME-Version: 1.0 17, 34 -- Content-Type: text/plain; 17, 34 -- charset="us-ascii" 17, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 34 -- Subject: RE: [Microscopy] shorted leadwires - current contrast? 17, 34 -- Date: Mon, 19 Mar 2007 09:03:16 -0000 17, 34 -- Message-ID: {9645D3E33E4C6548B12A7B25F611533E3E8EDB-at-cas-smx-02.BOOKHAM.ENTERPRISE.PRI} 17, 34 -- In-Reply-To: {200703161818.l2GIIQbo012103-at-ns.microscopy.com} 17, 34 -- X-MS-Has-Attach: 17, 34 -- X-MS-TNEF-Correlator: 17, 34 -- Thread-Topic: [Microscopy] shorted leadwires - current contrast? 17, 34 -- Thread-Index: Acdn94DfadbR11ASRxGhiQ8uwbRbBQCDFKNQ 17, 34 -- References: {200703161818.l2GIIQbo012103-at-ns.microscopy.com} 17, 34 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 17, 34 -- To: {opmills-at-mtu.edu} 17, 34 -- Cc: {microscopy-at-microscopy.com} 17, 34 -- X-OriginalArrivalTime: 19 Mar 2007 09:03:18.0883 (UTC) FILETIME=[6FF18B30:01C76A05] 17, 34 -- Content-Transfer-Encoding: 8bit 17, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2J93iRr027812 ==============================End of - Headers==============================
I recently was asked by someone that knew my background in materials, chemistry and electronics to consult on a problem involving very expensive printed circuit boards, pro bono. Anyway, I requested to use their real time video optical microscope to examine the defects and was also given a brief tour of the production facilities at one point.
One of the pieces of equipment I saw was a transmission X-ray microscope. It operated just like a transmission electron microscope with a real time on-line image analysis system and had impressive magnifications. I was shown a high density 12" by 16" circuit board with an surface mount technology (SMT) microprocessor socket on it. The large pin grid array socket pads were soldered underneath and normally invisible. This machine showed an X-ray view of how every SMT pad looked and if it was isolated, poorly soldered, non-wetted or shorted. the scan was stopped and I was shown a complete detailed morphology of a single soldered pad on a CRT as a demo. The image analysis process was highly automated and very fast. The operator could stop the machine and perform a manual inspection of any solder joint.
This machine surely is the way for you to go. It is nondestructive, fast, requires no coating, and they could save the real time images. I hope I gave you a taste of how powerful this system was. The image analysis software was incredible!
My point is that you can contact a surface mount printed circuit board manufacturer in your area and see they have this equipment. I am sure they could look at your wires with a very minimal disruption of their process. The question is, "Would they accept outside analytical work?" Some places won't because of legal issues.
Of course you could contact an X-ray microscope manufacturer and ask them for a demo on your real world sample. At the time, I was fascinated by how much this "table top" transmission X-ray microscope could do and how it worked just like my old CM-12 TEM with real time Quantimet on-line imaging. I never thought to get the manufacturer's name. It was a commercially built unit though.
Maybe someone on the list could arrange to run a sample for you as a "professional courtesy" at their technical center. Somebody in the electronics field and on this list must have access to one of these at their company or could arrange to run a sample for you. Intel comes to mind.
HTH,
Paul Beauregard 724-834-2247
} } -----Original Message----- } X-from: opmills-at-mtu.edu [mailto:opmills-at-mtu.edu] } Sent: 16 March 2007 18:18 } To: Richard Beanland } Subject: [Microscopy] shorted leadwires - current contrast? } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America
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We have a Supra 35VP, and just to let you know our SCM works as you expect - i.e. like your GW Faraday cup, changes due to KeV and apertures.
Secondly, I just checked, our SCM reads -2.5pA to -300fA without the KeV, and Status = underrange (vs Status = Normal).
May not help a lot but at least your know what normal seems to be.
On 16 Mar 2007 at 17:21, gary-at-gaugler.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all: } } I'm stumped by Faraday cup readings versus Zeiss } Specimen Current Meter (SCM) readings. } } For any aperture and any KV, SCM reads 75pA. } If I use the GW EBIC SCM, the values change } as expected. A Keithly picoampmeter also confirms } this. } } Why does the Zeiss SCM (Supra 55VP) not change? } It does on a specimen but not into the Faraday cup. } } gary g. } } } ==============================Original Headers============================== } 6, 17 -- From gary-at-gaugler.com Fri Mar 16 17:20:41 2007 } 6, 17 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) } 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2GMKfZK019796 } 6, 17 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 17:20:41 -0500 } 6, 17 -- Message-Id: {200703162220.l2GMKfZK019796-at-ns.microscopy.com} } 6, 17 -- Received: (qmail 17853 invoked from network); 16 Mar 2007 15:20:41 -0700 } 6, 17 -- Received: by simscan 1.1.0 ppid: 17848, pid: 17849, t: 0.1790s } 6, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 } 6, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) } 6, 17 -- by qsmtp1 with SMTP; 16 Mar 2007 15:20:41 -0700 } 6, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 6, 17 -- Date: Fri, 16 Mar 2007 15:20:35 -0800 } 6, 17 -- To: MSA listserver {microscopy-at-microscopy.com} } 6, 17 -- From: Gary Gaugler {gary-at-gaugler.com} } 6, 17 -- Subject: Faraday cup readings } 6, 17 -- Mime-Version: 1.0 } 6, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-ADA515D } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 11, 30 -- From edelmare-at-muohio.edu Mon Mar 19 12:19:25 2007 11, 30 -- Received: from spamfirewall.muohio.edu (wallaby.mcs.muohio.edu [134.53.6.28]) 11, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2JHJOSX032607 11, 30 -- for {microscopy-at-Microscopy.com} ; Mon, 19 Mar 2007 12:19:25 -0500 11, 30 -- X-ASG-Debug-ID: 1174324764-0ae4009e0000-Dem1zR 11, 30 -- X-Barracuda-URL: http://spamfirewall.muohio.edu:80/cgi-bin/mark.cgi 11, 30 -- X-Barracuda-Connect: mulnx23.mcs.muohio.edu[134.53.6.10] 11, 30 -- X-Barracuda-Start-Time: 1174324764 11, 30 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 11, 30 -- by spamfirewall.muohio.edu (Spam Firewall) with ESMTP 11, 30 -- id 699811A93A5; Mon, 19 Mar 2007 13:19:24 -0400 (EDT) 11, 30 -- Received: from [192.168.1.23] ([134.53.14.105]) 11, 30 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l2JHJLxg026272; 11, 30 -- Mon, 19 Mar 2007 13:19:23 -0400 11, 30 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 11, 30 -- To: gary-at-gaugler.com 11, 30 -- Date: Mon, 19 Mar 2007 13:19:24 -0400 11, 30 -- MIME-Version: 1.0 11, 30 -- X-ASG-Orig-Subj: Re: [Microscopy] Faraday cup readings 11, 30 -- Subject: Re: [Microscopy] Faraday cup readings 11, 30 -- CC: microscopy-at-Microscopy.com 11, 30 -- Message-ID: {45FE8DDC.7331.62D71A-at-edelmare.muohio.edu} 11, 30 -- Priority: normal 11, 30 -- In-reply-to: {200703162221.l2GMLOwL022138-at-ns.microscopy.com} 11, 30 -- References: {200703162221.l2GMLOwL022138-at-ns.microscopy.com} 11, 30 -- X-mailer: Pegasus Mail for Windows (4.41) 11, 30 -- Content-type: text/plain; charset=US-ASCII 11, 30 -- Content-transfer-encoding: 7BIT 11, 30 -- Content-description: Mail message body 11, 30 -- X-Barracuda-Virus-Scanned: by Barracuda Spam Firewall at muohio.edu ==============================End of - Headers==============================
Thanks for the feedback. The analysis was done into a Zeiss Faraday cup PN 348342-8055-000.
Zeiss SCM reads 75pA no matter what. GW SCM and Keithly read according to KV and apertures. But the Zeiss SCM reads OK onto regular specimens. Odd.
This could be a stage issue. I have the earlier Klein 5 stage and it leaves a lot to be desired. It is rather sloppy and will un-initialize at times. Zeiss said they were going to replace it but that never happened. My Amray 1910FE had an aperture in the stage for Faraday cup use and it worked fine using the external current meters. perhaps there is something wrong with the Zeiss Faraday cup. It is new and seems/looks OK. If I can find another supplier, I will try that and compare.
Since your Zeiss SCM works, it is puzzling at my end.
gary g.
At 09:21 AM 3/19/2007, you wrote:
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==============================Original Headers============================== 13, 20 -- From gary-at-gaugler.com Mon Mar 19 12:47:59 2007 13, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2JHlwPq012010 13, 20 -- for {microscopy-at-microscopy.com} ; Mon, 19 Mar 2007 12:47:58 -0500 13, 20 -- Message-Id: {200703191747.l2JHlwPq012010-at-ns.microscopy.com} 13, 20 -- Received: (qmail 22012 invoked from network); 19 Mar 2007 10:47:58 -0700 13, 20 -- Received: by simscan 1.1.0 ppid: 22009, pid: 22010, t: 0.0688s 13, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 13, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 13, 20 -- by qsmtp1 with SMTP; 19 Mar 2007 10:47:58 -0700 13, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 20 -- Date: Mon, 19 Mar 2007 10:47:54 -0800 13, 20 -- To: edelmare-at-muohio.edu 13, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 13, 20 -- Subject: Re: [Microscopy] Re: Faraday cup readings 13, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 13, 20 -- In-Reply-To: {200703191721.l2JHL9hP003072-at-ns.microscopy.com} 13, 20 -- References: {200703191721.l2JHL9hP003072-at-ns.microscopy.com} 13, 20 -- Mime-Version: 1.0 13, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-41A95EA2 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (connellyps-at-mail.nih.gov) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, March 19, 2007 at 12:56:49 ---------------------------------------------------------------------------
Email: connellyps-at-mail.nih.gov Name: Pat Connelly
Organization: NIH
Education: Graduate College
Location: Bethesda, MD
Question: The light on an RCM MTXL ultra-microtome from 1998 has gone out. I have replaced the light and althought the fiber optic light still works there is no light coming from the replaced bulb and the one removed does not seem to be burnt. Fuses were supplied with the microtome so I tried to locate them. The Operator's Manual does not show any fuse locations. There are no fuses in the control box nor any external fuse ports on the microtome body. Can anyone tell me where a fuse is and how to get to it. I have not taken an RCM microtome apart before. The support arm for the binoculars - does this need to be removed before taking off the housing? Help by phone or email would be most appreciated.
Thank you, Pat Connelly connellyps-at-mail.nih.gov 301-496-3491
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pmccurdy-at-lamar.colostate.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: pmccurdy-at-lamar.colostate.edu Name: Patrick McCurdy
Organization: Colorado State University
Title-Subject: [Filtered] Imaging blood cells on vinyl
Question: -What does it take to get pictures of cells (WBC, RBC, platelets) on the surface of vinyl film and depth filter media? -Is there an easy way to prepare the samples so the cells aren't significantly affected? -What do I need to do to be able to see the cell shape, cell types, and attachment through this filter depth media that has fibers ~ 2 microns in diameter? -What do I need to see the cells piled up in the texture on a vinyl film? -Would this all be possible with fluorescence microscope? -Can I scan a sample at low magnification ~300x to find areas of interest and look deeper?
Does anyone have a protocol for staining a 2 phase polymer film with ruthenium tetroxide? I need to try to stain a FIB cross section mounted on an omniprobe grid, so I'm thinking vapor staining, or possibly 'en bloc' staining of the film before I FIB it, it's about 30 nm thick on top of a silicon wafer. The finished cross section is roughly 50 nm or so sample thickness.
We've already tried vapor staining with OsO4, it has no interaction with this particular system.
Thank you, Leslie _____________________ Leslie Krupp IBM Almaden Research 650 Harry Road, K19/D1 San Jose, CA 95120-6099 (408) 927-3856
==============================Original Headers============================== 3, 27 -- From lkrupp-at-us.ibm.com Mon Mar 19 18:57:21 2007 3, 27 -- Received: from e2.ny.us.ibm.com (e2.ny.us.ibm.com [32.97.182.142]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2JNvKEM021603 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 19 Mar 2007 18:57:20 -0500 3, 27 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236]) 3, 27 -- by e2.ny.us.ibm.com (8.13.8/8.13.8) with ESMTP id l2JNvJod016416 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 19 Mar 2007 19:57:19 -0400 3, 27 -- Received: from d01av01.pok.ibm.com (d01av01.pok.ibm.com [9.56.224.215]) 3, 27 -- by d01relay04.pok.ibm.com (8.13.8/8.13.8/NCO v8.3) with ESMTP id l2JNvJBj314862 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 19 Mar 2007 19:57:19 -0400 3, 27 -- Received: from d01av01.pok.ibm.com (loopback [127.0.0.1]) 3, 27 -- by d01av01.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id l2JNvJYr003569 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 19 Mar 2007 19:57:19 -0400 3, 27 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) 3, 27 -- by d01av01.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id l2JNvJWf003566 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 19 Mar 2007 19:57:19 -0400 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- MIME-Version: 1.0 3, 27 -- Subject: ruthenium tetroxide staining recipe 3, 27 -- X-Mailer: Lotus Notes Release 7.0 HF277 June 21, 2006 3, 27 -- Message-ID: {OF3787E42C.EEA72620-ON852572A3.00833C42-882572A3.00839A7E-at-us.ibm.com} 3, 27 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} 3, 27 -- Date: Mon, 19 Mar 2007 16:57:18 -0700 3, 27 -- X-MIMETrack: Serialize by Router on D01ML604/01/M/IBM(Build V80_M4_03042007|March 04, 2007) at 3, 27 -- 03/19/2007 19:57:19, 3, 27 -- Serialize complete at 03/19/2007 19:57:19 3, 27 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (paigelassen-at-yahoo.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, March 9, 2007 at 10:09:46 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both paigelassen-at-yahoo.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: Good morning. I have inherited a microscope and considering selling it. My dad acquired it from a pathologist for medical school back in 1960. If you could provide information about the microscope or direct me to a service that appraises microscopes, it would be much appreciated. It is an Ernst Leitz GmbH/Wetzlar Laborlux. Serial #(?) is 519 465. Has all original components, slides, box, and papers written in German. Thank you in advance for any recommendations.
Postdoc – (USA West coast based) – for a collaboration between Portland State University, the University of California at Davis, the University of Washington at Seattle, and the Pacific NorthWest National Laboratory
Initially for one year at $35,500 plus fringe benefits (health insurance, retirement benefits, etc.), available from May 1st, 2007, onwards, extendable up to 3 years by mutual agreement.
A group of collaborators from Portland State University, the University of California at Davis, the University of Washington at Seattle, and the Pacific Northwest National Laboratory is seeking a (male/female) postdoc for a project on Crystallographic and spectroscopic analyses of ferromagnetic semiconducting nanoparticle aggregates with Curie temperatures well above room temperature {http://www.physics.pdx.edu/%7Epmoeck/projects/Daniels%20project%20short.htm} .
A background in materials physics, materials chemistry, crystallography, or materials science and engineering is required. Familiarity with Z-contrast (HAADF) imaging in scanning transmission electron microscopes and associated electron energy loss spectroscopy are essential. Skills in high resolution phase-contrast transmission electron microscopy, electron diffraction and crystallography, Rietveld analysis, and crystallographic image processing will be appreciated.
The search will be open until the position has been filled. Applications (CV, list of referees, reasons for coming to the US for applicants from aboard, list of publications if applicable, etc.) should be sent to any or both the following collaborators:
Prof. Peter Moeck {http://www.physics.pdx.edu/%7Epmoeck/}
Department of Physics Portland State University P.O. Box 751 Portland, Oregon 97207-0751 Tel.: 503 725 4227 Fax: 503 725 2815
Does anyone use the Jeol JSM6400F SEM? I am going to attach a Auger Spectrumeter to this SEM, but I don't know how to control the SEM, for example, controling the electron beam scanning, the magnification etc. There is another EDS instrument connected to this SEM. It use the 'JA2' port in the SEM,which is a 14 pins interface. This interface looks like a GPIB one from the outlook. If anyone know anything concerning this interface and the way to control the SEM, please give me a hand.
Many thanks Charles
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==============================Original Headers============================== 6, 21 -- From charlesping-at-hotmail.com Tue Mar 20 05:25:38 2007 6, 21 -- Received: from bay0-omc2-s6.bay0.hotmail.com (bay0-omc2-s6.bay0.hotmail.com [65.54.246.142]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2KAPc88003660 6, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Mar 2007 05:25:38 -0500 6, 21 -- Received: from hotmail.com ([64.4.51.14]) by bay0-omc2-s6.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2668); 6, 21 -- Tue, 20 Mar 2007 03:25:37 -0700 6, 21 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 6, 21 -- Tue, 20 Mar 2007 03:25:37 -0700 6, 21 -- Message-ID: {BAY107-F4A025540553145DFAF712BD750-at-phx.gbl} 6, 21 -- Received: from 64.4.51.220 by by107fd.bay107.hotmail.msn.com with HTTP; 6, 21 -- Tue, 20 Mar 2007 10:25:35 GMT 6, 21 -- X-Originating-IP: [144.32.136.9] 6, 21 -- X-Originating-Email: [charlesping-at-hotmail.com] 6, 21 -- X-Sender: charlesping-at-hotmail.com 6, 21 -- From: "xiaoping zha" {charlesping-at-hotmail.com} 6, 21 -- To: Microscopy-at-microscopy.com 6, 21 -- Subject: JEOL 6400F SEM need help for the interface 6, 21 -- Date: Tue, 20 Mar 2007 10:25:35 +0000 6, 21 -- Mime-Version: 1.0 6, 21 -- Content-Type: text/plain; format=flowed 6, 21 -- X-OriginalArrivalTime: 20 Mar 2007 10:25:37.0843 (UTC) FILETIME=[1A34C830:01C76ADA] ==============================End of - Headers==============================
Personally I wonder why it would not be possible to do it with a confocal scanning microscope. Molecular probes makes whole cell fluorescent markers. Although I have never done 3D reconstruction with a confocal microscope, it is pretty well adapted for this purpose. But you have to consider the penetration of the laser beam in the filter.
See for ex.:
Rothen-Rutishauser B et al. Formation of multilayers in the caco-2 cell culture model: a confocal laser scanning microscopy study. Pharm Res. 2000 Apr;17(4):460-5.
regards,
Stephane
--- pmccurdy-at-lamar.colostate.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the } Microscopy Listserver } using the WWW based Form at } http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a } Subscriber, so when replying } please copy both pmccurdy-at-lamar.colostate.edu as } well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: pmccurdy-at-lamar.colostate.edu } Name: Patrick McCurdy } } Organization: Colorado State University } } Title-Subject: [Filtered] Imaging blood cells on } vinyl } } Question: -What does it take to get pictures of } cells (WBC, RBC, } platelets) on the } surface of vinyl film and depth filter media? } -Is there an easy way to prepare the samples so the } cells aren't } significantly affected? } -What do I need to do to be able to see the cell } shape, cell types, and } attachment through this filter depth media that has } fibers ~ 2 microns } in diameter? } -What do I need to see the cells piled up in the } texture on a vinyl } film? } -Would this all be possible with fluorescence } microscope? } -Can I scan a sample at low magnification ~300x to } find areas of } interest and look deeper? } } } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 7, 13 -- From zaluzec-at-microscopy.com Mon Mar 19 } 14:28:00 2007 } 7, 13 -- Received: from [172.21.1.183] } (msdvpn8.msd.anl.gov [130.202.238.72]) } 7, 13 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l2JJRtBK004727 } 7, 13 -- for {microscopy-at-microscopy.com} ; Mon, 19 } Mar 2007 14:27:56 -0500 } 7, 13 -- Mime-Version: 1.0 } 7, 13 -- X-Sender: (Unverified) } 7, 13 -- Message-Id: } {p06020403c2249669f9da-at-[172.21.1.183]} } 7, 13 -- Date: Mon, 19 Mar 2007 14:35:20 -0500 } 7, 13 -- To: microscopy-at-microscopy.com } 7, 13 -- From: pmccurdy-at-lamar.colostate.edu (by way } of MicroscopyListserver) } 7, 13 -- Subject: [Filtered] } MicroscopyListserverviaWWW: Imaging blood cells on } 7, 13 -- vinyl } 7, 13 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } ==============================End of - } Headers============================== }
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==============================Original Headers============================== 11, 21 -- From nizets2-at-yahoo.com Tue Mar 20 07:03:53 2007 11, 21 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2KC3rng017060 11, 21 -- for {microscopy-at-microscopy.com} ; Tue, 20 Mar 2007 07:03:53 -0500 11, 21 -- Received: (qmail 20310 invoked by uid 60001); 20 Mar 2007 12:03:52 -0000 11, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 21 -- s=s1024; d=yahoo.com; 11, 21 -- h=X-YMail-OSG:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 11, 21 -- b=DgQUSOWo3vOEPt0Xuu0tDDMF+zkpcO5oMYwaP+kdTThB9rLXX8WuKT4qDL53SRZHwczudHZu6TjXy8a8YpX0N1EqhY48d6wDgmD/BSfG/c9rQ4ptomECL7MuJxlEIRKWAIaHYnG/qP5JlDN2P5u7Um2S+ohxSlj1Y+wYTO/U1rw=; 11, 21 -- X-YMail-OSG: fNAiDRsVM1k31XHEq7uk3qVRtkx9AyKZoDuFxjUQDSgYX96uKj1VlkvMupTtaChlJ1Xr35apThVMP8yRwZyMApR9klgNNiZcPfDr7ybVB4.fWtRr4A1LOceZXP1NP1XCP5fXRfzKIZtlOGHqE8V4FmwObGHANLCfbjmkSQ0wWnLhoLVJtPCXoX0Oo0ojDZ4Mw9HrVEaS0fDY7nVx.f4- 11, 21 -- Received: from [80.122.101.102] by web37406.mail.mud.yahoo.com via HTTP; Tue, 20 Mar 2007 05:03:52 PDT 11, 21 -- Date: Tue, 20 Mar 2007 05:03:52 -0700 (PDT) 11, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 11, 21 -- Subject: Re: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Imaging blood cells on 11, 21 -- To: pmccurdy-at-lamar.colostate.edu 11, 21 -- Cc: microscopy-at-microscopy.com 11, 21 -- In-Reply-To: {200703191931.l2JJV2A2012566-at-ns.microscopy.com} 11, 21 -- MIME-Version: 1.0 11, 21 -- Content-Type: text/plain; charset=iso-8859-1 11, 21 -- Content-Transfer-Encoding: 8bit 11, 21 -- Message-ID: {786929.19631.qm-at-web37406.mail.mud.yahoo.com} ==============================End of - Headers==============================
I'm not intimately familiar with the SEM you are referencing, but my first question is: Does this SEM run in the ultra-high vacuum range? I was not aware that it could.
dj
On Tue, 20 Mar 2007, charlesping-at-hotmail.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hi there } } Does anyone use the Jeol JSM6400F SEM? I am going to attach a Auger } Spectrumeter to this SEM, but I don't know how to control the SEM, for } example, controling the electron beam scanning, the magnification etc. } There is another EDS instrument connected to this SEM. It use the 'JA2' port } in the SEM,which is a 14 pins interface. This interface looks like a GPIB } one from the outlook. } If anyone know anything concerning this interface and the way to control the } SEM, please give me a hand. } } Many thanks } Charles } } _________________________________________________________________ } Watch free concerts with Pink, Rod Stewart, Oasis and more. Visit MSN } Presents today. } http://music.msn.com/presents?icid=ncmsnpresentstagline&ocid=T002MSN03A07001 } } } ==============================Original Headers============================== } 6, 21 -- From charlesping-at-hotmail.com Tue Mar 20 05:25:38 2007 } 6, 21 -- Received: from bay0-omc2-s6.bay0.hotmail.com (bay0-omc2-s6.bay0.hotmail.com [65.54.246.142]) } 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2KAPc88003660 } 6, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Mar 2007 05:25:38 -0500 } 6, 21 -- Received: from hotmail.com ([64.4.51.14]) by bay0-omc2-s6.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2668); } 6, 21 -- Tue, 20 Mar 2007 03:25:37 -0700 } 6, 21 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; } 6, 21 -- Tue, 20 Mar 2007 03:25:37 -0700 } 6, 21 -- Message-ID: {BAY107-F4A025540553145DFAF712BD750-at-phx.gbl} } 6, 21 -- Received: from 64.4.51.220 by by107fd.bay107.hotmail.msn.com with HTTP; } 6, 21 -- Tue, 20 Mar 2007 10:25:35 GMT } 6, 21 -- X-Originating-IP: [144.32.136.9] } 6, 21 -- X-Originating-Email: [charlesping-at-hotmail.com] } 6, 21 -- X-Sender: charlesping-at-hotmail.com } 6, 21 -- From: "xiaoping zha" {charlesping-at-hotmail.com} } 6, 21 -- To: Microscopy-at-microscopy.com } 6, 21 -- Subject: JEOL 6400F SEM need help for the interface } 6, 21 -- Date: Tue, 20 Mar 2007 10:25:35 +0000 } 6, 21 -- Mime-Version: 1.0 } 6, 21 -- Content-Type: text/plain; format=flowed } 6, 21 -- X-OriginalArrivalTime: 20 Mar 2007 10:25:37.0843 (UTC) FILETIME=[1A34C830:01C76ADA] } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 19 -- From dljones-at-bestweb.net Tue Mar 20 07:48:50 2007 6, 19 -- Received: from vms048pub.verizon.net (vms048pub.verizon.net [206.46.252.48]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2KCmoA6029414 6, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Mar 2007 07:48:50 -0500 6, 19 -- Received: from localhost ([162.84.216.151]) 6, 19 -- by vms048.mailsrvcs.net (Sun Java System Messaging Server 6.2-6.01 (built Apr 6, 19 -- 3 2006)) with ESMTPA id {0JF700BAACWXXNUI-at-vms048.mailsrvcs.net} for 6, 19 -- Microscopy-at-microscopy.com; Tue, 20 Mar 2007 07:48:38 -0500 (CDT) 6, 19 -- Date: Tue, 20 Mar 2007 08:48:36 -0400 (Eastern Daylight Time) 6, 19 -- From: "David L. Jones" {dljones-at-bestweb.net} 6, 19 -- Subject: Re: [Microscopy] JEOL 6400F SEM need help for the interface 6, 19 -- In-reply-to: {200703201031.l2KAVI8B009988-at-ns.microscopy.com} 6, 19 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 6, 19 -- To: charlesping-at-hotmail.com 6, 19 -- Cc: Microscopy-at-microscopy.com 6, 19 -- Message-id: {Pine.WNT.4.64.0703200837450.3356-at-H-F1} 6, 19 -- MIME-version: 1.0 6, 19 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 6, 19 -- References: {200703201031.l2KAVI8B009988-at-ns.microscopy.com} ==============================End of - Headers==============================
Does your JEOL SEM have a UHV sample chamber? Auger spectrometers are generally used only in UHV systems. Since the sampling depth is on the order of a few nm (or less), any surface layers will affect your results. An old vacuum rule of thumb is that you get 1 monolayer of contamination (oxide, etc.) per second at e-6 torr. Hence at 1e-9 torr, you have ~1000 seconds (20 minutes) before your pristine surface is no more. Most Auger systems also have Ar sputtering guns to clean the surface just before the analysis.
The vacuum in most SEM chambers is not adequate to do Auger work.
Cheers, Henk
At 06:28 AM 03/20/07, you wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 10, 26 -- From colijn.1-at-osu.edu Tue Mar 20 08:45:55 2007 10, 26 -- Received: from ER6S1.ENG.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2KDjt9x009991 10, 26 -- for {microscopy-at-microscopy.com} ; Tue, 20 Mar 2007 08:45:55 -0500 10, 26 -- Received: from CONVERSION-DAEMON.er6s1.eng.ohio-state.edu by 10, 26 -- er6s1.eng.ohio-state.edu (PMDF V6.2-1x11 #31056) 10, 26 -- id {01MEFR8JOOKGAAKJ0D-at-er6s1.eng.ohio-state.edu} for 10, 26 -- microscopy-at-microscopy.com; Tue, 20 Mar 2007 09:45:54 -0400 (EDT) 10, 26 -- Received: from HOC1.ecr6.ohio-state.edu 10, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.eng.ohio-state.edu 10, 26 -- (PMDF V6.2-1x11 #31056) 10, 26 -- with ESMTPA id {01MEFR8J9382AANWZ3-at-er6s1.eng.ohio-state.edu} ; Tue, 10, 26 -- 20 Mar 2007 09:45:54 -0400 (EDT) 10, 26 -- Date: Tue, 20 Mar 2007 09:49:07 -0400 10, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 10, 26 -- Subject: Re: [Microscopy] JEOL 6400F SEM need help for the interface 10, 26 -- In-reply-to: {200703201028.l2KASKlv006378-at-ns.microscopy.com} 10, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 10, 26 -- To: charlesping-at-hotmail.com 10, 26 -- Cc: microscopy-at-microscopy.com 10, 26 -- Message-id: {7.0.1.0.2.20070320093445.03469f78-at-osu.edu} 10, 26 -- MIME-version: 1.0 10, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 10, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 10, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 10, 26 -- References: {200703201028.l2KASKlv006378-at-ns.microscopy.com} ==============================End of - Headers==============================
Justin, Unless they are paying for the shipping, you're going to be out a sizeable chunk of change just in the shipping costs. If someone doesn't spend at least a few hours properly packing the system for however it is going to be shipped (and how it will be shipped makes a difference in how it is packed), you will receive a large anchor for your boat. Unless you have someone at your end who is experienced and willing to donate their time for getting this system back up and running, you probably don't have the budget to get it running if you don't have the budget to have it packed properly.
I hate to be so down on this because I currently support an SEM at a high school because I firmly believe that having the students have the opportunity to play with the toys that we get to play with in the real world is a great way to inspire them to get the education they need. However, in addition to not seeing some major components to the system in your photos (you also need someone to evalute what's there), I can tell you that I once packed a system for a customer on a tight budget. I packed it for shipment by padded van (which is a much less intensive packing job than for a basic truck), but they decided to just put it on an Estes 18 wheeler despite what I had told them. By the time it had gone from PA to CA it was trashed. Penny wise and pound foolish. And they had PAID for that system!
Unfortunately aquiring a free SEM is an expensive affair unless you can find someone to do it all properly because THEY are also committed to the students. It's a sizeable commitment to take on even one SEM pro-bono, but maybe someone in your area could contact you, if they're interested. Personally, I would like to see the manufacturers take on a high school system pro-bono for every x number of field service engineers, but that probably won't ever happen. (Are you guys paying attention, here?).
I sincerely hope that someone DOES contact you and I also hope that my doom and gloom is off the mark.
Best wishes Ken Converse Owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com] Sent: Saturday, March 17, 2007 2:28 PM To: kenconverse-at-qualityimages.biz
} This Question was submitted to Ask-A-Microscopist by (paigelassen-at-yahoo.com) } from } http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html } on Friday, March 9, 2007 at 10:09:46 } Remember to consider the Grade/Age of the student when considering } the Question } --------------------------------------------------------------------------- } Please reply to both paigelassen-at-yahoo.com as well as to the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: paigelassen-at-yahoo.com } Name: Paige Lassen } } Organization: KCUMB } } Education: Graduate College } } Location: Newark, NJ, USA } } Title: appraisal of vintage microscope } } Question: Good morning. I have inherited a microscope and } considering selling it. My dad acquired it from a pathologist for } medical school back in 1960. If you could provide information about } the microscope or direct me to a service that appraises microscopes, } it would be much appreciated. } It is an Ernst Leitz GmbH/Wetzlar Laborlux. Serial #(?) is 519 465. } Has all original components, slides, box, and papers written in } German. Thank you in advance for any recommendations. } } --------------------------------------------------------------------------- } The New York Microscopy Society meets in Montclair, New Jersey; they have an extensive microscope collection and a lot of expertise. Contact Don Oleary {donoleary-at-worldnet.att.net} for advice. -- Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 1, 19 -- From schooley-at-mcn.org Tue Mar 20 10:48:56 2007 1, 19 -- Received: from dns4.mcn.org (dns4.mcn.org [216.150.240.31]) 1, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2KFmuYi003640 1, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 20 Mar 2007 10:48:56 -0500 1, 19 -- Received: from [66.52.170.119] 1, 19 -- by dns4.mcn.org with esmtpa (Exim 4.60) 1, 19 -- (envelope-from {schooley-at-mcn.org} ) 1, 19 -- id JF7L9I-000DH3-4D; Tue, 20 Mar 2007 08:48:55 -0700 1, 19 -- Mime-Version: 1.0 1, 19 -- Message-Id: {a06200700c225b1f30927-at-[66.52.170.114]} 1, 19 -- In-Reply-To: {200703200244.l2K2iQVL011942-at-ns.microscopy.com} 1, 19 -- References: {200703200244.l2K2iQVL011942-at-ns.microscopy.com} 1, 19 -- Date: Tue, 20 Mar 2007 08:50:17 -0700 1, 19 -- To: paigelassen-at-yahoo.com 1, 19 -- From: Caroline Schooley {schooley-at-mcn.org} 1, 19 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: appraisal of 1, 19 -- vintage microscope 1, 19 -- Cc: Microscopy-at-MSA.Microscopy.Com 1, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
The recipe for preparing ruthenium tetroxide in-situ, as well as other important tips, follows.
Important safety note: RuO4 vapors is a very strong oxidizer. Therefore, all work involved in the preparation, use and disposal RuO4 must be done in a good exhaust hood (} 100 cfm exhaust). Safety glasses with side-shields (or goggles) and gloves (nitrile is best) is a must.
To make the stain, weigh 0.02g of ruthenium trichloride hydrate (RuCl3-xH2O, CAS 14898-67-0) into a small (5 ml) vial. Add add 1 ml of (NaOCl (10-13 %, CAS 7681-52-9) and agitate with a Pasteur pipet until the RuCl3-xH2O has dissolved. The resulting solution should be deep reddish brown. Cap the vial immediately. I generally affix the sample to be stained to the inside of the vial cap and stain for the prescribed amount of time. The duration of staining for your polymer blend will have to be determined empirically. To safely dispose the stain following use, reduce by adding an excess of 10-15% aqueous sodium bisulfite (NaHSO3, CAS 7631-90-5); the reduced solution will become pale green to blue overnight, at which time it can be properly disposed.
All chemicals are available from Sigma-Aldrich and (presumably) other vendors. Although I recommend the NaOCl noted above, in a pinch, household bleach may be used instead of the reagent grade variety. The concentration of household bleach does decrease with shelf life so be sure to purchase a new jug with a long expiration date. If you plan to use this procedure over a period of time, purchase the good stuff and keep it refrigerated. The advantage of using 10-13% reagent-grade NaOCl is that a usable concentration of the NaOCl remains in solution over a longer period of time. Always refrigerate to prolong shelf-life.
Prior to staining, one must first know the composition of the polymer blend and which phase is preferentially stains by RuO4. You will have to empirically determine whether staining should be performed en bloc or following FIB milling.
Several resources on RuO4 staining of polymers are available to you: (1) A good reference for the selectivity of various stains is "Polymer Microscopy" by Sawyer and Grubbs (3rd ed.?). (2) Two papers on the subject are authored by Trent et al. and Montezinos and are referenced in the book. (3) I included an in-depth appendix on the details of stain preparation and use in G. M. Brown and J. H. Butler, “New method for the characterization of domain morphology of polymer blends using ruthenium tetroxide staining and low voltage scanning electron microscopy (LVSEM)â€, Polymer 38 (15), 3937 (1997).
You may write or call me off-line as needed.
Regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Happiness equals reality minus expectations." Tom Magliozzi
lkrupp-at-us.ibm. com To gary.m.brown-at-exxonmobil.com 03/19/07 07:00 cc PM Subject [Microscopy] ruthenium tetroxide Please respond staining recipe to lkrupp-at-us.ibm. com
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Does anyone have a protocol for staining a 2 phase polymer film with ruthenium tetroxide? I need to try to stain a FIB cross section mounted on an omniprobe grid, so I'm thinking vapor staining, or possibly 'en bloc' staining of the film before I FIB it, it's about 30 nm thick on top of a silicon wafer. The finished cross section is roughly 50 nm or so sample thickness.
We've already tried vapor staining with OsO4, it has no interaction with this particular system.
Thank you, Leslie _____________________ Leslie Krupp IBM Almaden Research 650 Harry Road, K19/D1 San Jose, CA 95120-6099 (408) 927-3856
==============================Original Headers============================== 3, 27 -- From lkrupp-at-us.ibm.com Mon Mar 19 18:57:21 2007 3, 27 -- Received: from e2.ny.us.ibm.com (e2.ny.us.ibm.com [32.97.182.142]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2JNvKEM021603 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 19 Mar 2007 18:57:20 -0500 3, 27 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236]) 3, 27 -- by e2.ny.us.ibm.com (8.13.8/8.13.8) with ESMTP id l2JNvJod016416 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 19 Mar 2007 19:57:19 -0400 3, 27 -- Received: from d01av01.pok.ibm.com (d01av01.pok.ibm.com [9.56.224.215]) 3, 27 -- by d01relay04.pok.ibm.com (8.13.8/8.13.8/NCO v8.3) with ESMTP id l2JNvJBj314862 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 19 Mar 2007 19:57:19 -0400 3, 27 -- Received: from d01av01.pok.ibm.com (loopback [127.0.0.1]) 3, 27 -- by d01av01.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id l2JNvJYr003569 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 19 Mar 2007 19:57:19 -0400 3, 27 -- Received: from d01ml604.pok.ibm.com (d01ml604.pok.ibm.com [9.56.227.90]) 3, 27 -- by d01av01.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id l2JNvJWf003566 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 19 Mar 2007 19:57:19 -0400 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- MIME-Version: 1.0 3, 27 -- Subject: ruthenium tetroxide staining recipe 3, 27 -- X-Mailer: Lotus Notes Release 7.0 HF277 June 21, 2006 3, 27 -- Message-ID: {OF3787E42C.EEA72620-ON852572A3.00833C42-882572A3.00839A7E-at-us.ibm.com} 3, 27 -- From: Leslie E Krupp {lkrupp-at-us.ibm.com} 3, 27 -- Date: Mon, 19 Mar 2007 16:57:18 -0700 3, 27 -- X-MIMETrack: Serialize by Router on D01ML604/01/M/IBM(Build V80_M4_03042007|March 04, 2007) at 3, 27 -- 03/19/2007 19:57:19, 3, 27 -- Serialize complete at 03/19/2007 19:57:19 3, 27 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
==============================Original Headers============================== 29, 21 -- From gary.m.brown-at-exxonmobil.com Tue Mar 20 11:44:50 2007 29, 21 -- Received: from hoespc01.exxonmobil.com (hoespc01.exxonmobil.com [192.67.48.38]) 29, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2KGiots018007 29, 21 -- for {microscopy-at-microscopy.com} ; Tue, 20 Mar 2007 11:44:50 -0500 29, 21 -- Received: from dalnmg04.na.xom.com (dalnmg04.na.xom.com [131.126.97.123]) 29, 21 -- by hoespc01.exxonmobil.com (Switch-3.1.11/Switch-3.1.11) with ESMTP id l2KGikYq027540 29, 21 -- for {microscopy-at-microscopy.com} ; Tue, 20 Mar 2007 11:44:49 -0500 (CDT) 29, 21 -- In-Reply-To: {200703200000.l2K00Heq025053-at-ns.microscopy.com} 29, 21 -- Subject: Re: [Microscopy] ruthenium tetroxide staining recipe 29, 21 -- Importance: 29, 21 -- To: lkrupp-at-us.ibm.com, microscopy-at-microscopy.com 29, 21 -- X-Mailer: Lotus Notes 652HF109 January 14, 2005 29, 21 -- Message-ID: {OF840334FB.E5694DDA-ON862572A4.00572AC9-862572A4.005BFDB3-at-exxonmobil.com} 29, 21 -- From: gary.m.brown-at-exxonmobil.com 29, 21 -- Date: Tue, 20 Mar 2007 11:44:46 -0500 29, 21 -- X-MIMETrack: Serialize by Router on Dalnmg04.na.xom.com/S/ExxonMobil(652FP1HF193|March 29, 21 -- 02, 2006) at 03/20/2007 11:44:49 AM 29, 21 -- MIME-Version: 1.0 29, 21 -- Content-type: text/plain; charset=UTF-8 29, 21 -- Content-Transfer-Encoding: 8bit 29, 21 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id l2KGiots018007 ==============================End of - Headers==============================
It has been my experience that Ken is quite knowledgeable on this subject. His observations and suggestions I would take very seriously. He has clearly taken some time to look over your pictures and gave you some excellent comments.
I would also reiterate that, calling it the doom and gloom scenario or not, it would be very difficult for this instrument to arrive without damage if it is not packaged correctly.
No matter how it is shipped, it will cost money, likely in the 4 digit range of dollars. Hopefully for you, in the lower end of that range...
X-from personal experience, I can only suggest that either you find someone that can package this for you, or do it yourself. I don't know if you have the knowledge or experience. If you don't, some of us would be willing to give you free advice as to how better to try and have this instrument arrive in working order.
I have shipped many kinds of instruments from across the hall to across the ocean. Moving them presents problems. Proper packaging helps minimize those problems. Lack of packaging is a recipe for disaster.
I have gotten two SEM's donated to my local high school. In both cases, I personally went to where they were, packaged them myself and shipped them myself. It was the only way I could afford to do it. It worked. My daughter's high school can now give their students college credits straight from high school.
It is not easy. These instruments are not greeted necessarily with open arms by the administrators of schools. They are expensive to run, maintain and require specialized knowledge to operate them.
I really like Ken's comment on having SEM manufacture's donate technical expertise to schools to get these integrated into high schools. Our children will do nothing but benefit from this. Our industry will do nothing but benefit from this. The SEM manufacturers will benefit from this. It is a win-win situation.
dj
On Tue, 20 Mar 2007, kenconverse-at-qualityimages.biz wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Justin, } Unless they are paying for the shipping, you're going to be out a sizeable } chunk of change just in the shipping costs. If someone doesn't spend at } least a few hours properly packing the system for however it is going to be } shipped (and how it will be shipped makes a difference in how it is packed), } you will receive a large anchor for your boat. Unless you have someone at } your end who is experienced and willing to donate their time for getting } this system back up and running, you probably don't have the budget to get } it running if you don't have the budget to have it packed properly. } } I hate to be so down on this because I currently support an SEM at a high } school because I firmly believe that having the students have the } opportunity to play with the toys that we get to play with in the real world } is a great way to inspire them to get the education they need. However, in } addition to not seeing some major components to the system in your photos } (you also need someone to evalute what's there), I can tell you that I once } packed a system for a customer on a tight budget. I packed it for shipment } by padded van (which is a much less intensive packing job than for a basic } truck), but they decided to just put it on an Estes 18 wheeler despite what } I had told them. By the time it had gone from PA to CA it was trashed. } Penny wise and pound foolish. And they had PAID for that system! } } Unfortunately aquiring a free SEM is an expensive affair unless you can find } someone to do it all properly because THEY are also committed to the } students. It's a sizeable commitment to take on even one SEM pro-bono, but } maybe someone in your area could contact you, if they're interested. } Personally, I would like to see the manufacturers take on a high school } system pro-bono for every x number of field service engineers, but that } probably won't ever happen. (Are you guys paying attention, here?). } } I sincerely hope that someone DOES contact you and I also hope that my doom } and gloom is off the mark. } } Best wishes } Ken Converse } Owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } } -----Original Message----- } X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com] } Sent: Saturday, March 17, 2007 2:28 PM } To: kenconverse-at-qualityimages.biz } Subject: Re: [Microscopy] RE: New SEM donated to our lab- Can you identify } the accessory? } } } It's in New Jersey right now, and unfortunately we don't have the budget to } send someone up there to pack it properly. The company who is donating it } will not spend more than a few minutes prepping it (They aren't making money } off of it, and they are electronics recyclers, so they just see it as a pile } of metal) so I need to make instructions as simple and clear as possible, } without having too much for them to do, or they may just decide to Ebay the } whole thing. } } --Justin. } } On 3/17/07, kenconverse-at-qualityimages.biz {kenconverse-at-qualityimages.biz} } wrote: } } } } } } } } ---------------------------------------------------------------------- } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Justin, } } I believe what you have is a JXA-840 (microprobe) with 2 wavelength } } spectrometers and a light microscope for establishing the proper WD } } for using those spectrometers. The Box on top is an ion pump so that } } the system can be used with a LaB6 cathode, if you choose (and have } } received the correct wehnelt cap). } } } } Whether it is an 840 or 840A could be told by a look at the } } electronics console, but you don't have any pictures of that or the } } electronics rack that should be present for the spectometers. I do } } see the power supply console in a couple of pictures. Does it include } } the rotary pump and possibly compressor? } } } } As far as prepping for shipping, the first thing that jumps out at me } } is that the table isn't bolted down! At a minimum you need to get 4 } } M16x2.00x40mm bolts and 16mm washers to secure the table. You also } } need to remove the magnet from the ion pump. Actually, most of the } } column should be removed and packed separately unless you're planning } } to move it only a few miles at very low speed. I also suspect that } } the WDS units and light microscope should be removed. All 3 are } } complex and fragile. } } } } Is it diffusion pumped or turbo pumped? If it's turbo pumped, the } } pump must be removed before shipping. Also the high voltage oil tank } } in the electronics console must be removed and the circuit board on } } its side protected from damage. } } } } This is going to the W. Palm Beach area of Florida. Where is it } } currently located? I would highly recommend sending someone to pack } } it properly. How is it going to be shipped? If it's going by truck, } } it must be "padded van" unless you're going to have a lot more of it } } disassembled, crated and put on pallets. } } } } With more info, I might be of more help. } } } } Ken Converse } } owner } } } } QUALITY IMAGES } } Servicing Scanning Electron Microscopes } } Since 1981 } } 474 So. Bridgton Rd. } } Bridgton, ME 04009 } } 207-647-4348 } } Fax 207-647-2688 } } kenconverse-at-qualityimages.biz } } qualityimages.biz } } } } } } } } -----Original Message----- } } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] } } Sent: Friday, March 16, 2007 6:43 PM } } To: kenconverse-at-qualityimages.biz } } Subject: [Microscopy] New SEM donated to our lab- Can you identify the } } accessory? } } } } } } } } } } } } ---------------------------------------------------------------------- } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Hello all, } } } } We have a new SEM on the way to us, it's a JEOL JSM-840. All I have } } at this point are a couple of pictures, and I am a little flummoxed } } about the accessories that are added on this instrument. I recognize } } the secondary electron detector, and I believe that there is a } } backscatter detector on it, but I'm not sure what the other } } accessories are. Also, the photos that I have seen of the basic 840s } } don't include a box sitting on top of the column, but this one has it. } } It's a silver-ish box with black sides, and is barely visible in one } } of the photos, but it is there, at a slight angle. (Which leads me to } } the next question- how should it be secured for } } shipping?) } } } } The whole microscope is being packed by people who don't necessarily } } know how to package it. Do any of you have specific suggestions of } } what they could do to prevent damage in shipping? } } } } Here is the URL to the photos of the microscope (Hosted on my personal } } page which has not been updated in a long time...) } } http://www.jkraft.net/JEOL/ } } } } Thanks, } } } } Justin A. Kraft } } Director } } Center for Inquiry-Based Science Education } } } } ==============================Original } } Headers============================== } } 6, 26 -- From kraftpiano-at-gmail.com Fri Mar 16 17:40:22 2007 } } 6, 26 -- Received: from ik-out-1112.google.com (ik-out-1112.google.com } } [66.249.90.177]) } } 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id } } l2GMeL9w006622 } } 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 17:40:22 } } -0500 } } 6, 26 -- Received: by ik-out-1112.google.com with SMTP id b32so541347ika } } 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 } 15:40:20 } } -0700 (PDT) } } 6, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; } } 6, 26 -- d=gmail.com; s=beta; } } 6, 26 -- } } } h=domainkey-signature:received:received:message-id:date:from:to:subject:mime } } -version:content-type:content-transfer-encoding:content-disposition; } } 6, 26 -- } } } b=s1DejygYHazI0gOXcGdlH3aFhc31xI8o6mt+j5GOcTzrxtJPW9PLWzsfWcdgsZQjbUfGwio8NU } } weB8pHDxwgatI0zP5kv+TIfnL1G2A6U75mJ90Wu2/xog0gZFV7g5sBV3vJe5jiayVV4dGS } } weB8pHDxwgatI0zP5kv+kp74tv } } q2VQyDtZdd3Icvrv93WZs= } } 6, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } } 6, 26 -- d=gmail.com; s=beta; } } 6, 26 -- } } h=received:message-id:date:from:to:subject:mime-version:content-type:c } } ontent } } -transfer-encoding:content-disposition; } } 6, 26 -- } } } b=pR3ppcp1LBgkdhNL2mxb8fxkll/KXvwAFHADQW/u6FINK4J15EsY55ex1+B2+g0JNvff5T3gaa } } YDWFX6PzBDM720L+slCXtxDVdzFMBAPEocfGL+xIvvPiSQg1ACUQ8+ujyW+Q/J0di/cxKy } } YDWFX6PzBDM720L+slCXtxDVdzFMBAPEocfGL+xIvvPiSQg1ACUQ8+ujyW+HEXk7+ } } jE105VLOBzCHBFkml2E+0= } } 6, 26 -- Received: by 10.114.171.1 with SMTP id } t1mr918673wae.1174084819773; } } 6, 26 -- Fri, 16 Mar 2007 15:40:19 -0700 (PDT) } } 6, 26 -- Received: by 10.114.79.12 with HTTP; Fri, 16 Mar 2007 } } 15:40:19 -0700 (PDT) 6, 26 -- Message-ID: } } {25e2b0d20703161540k140001d5k6170427b9d645d6b-at-mail.gmail.com} } } 6, 26 -- Date: Fri, 16 Mar 2007 18:40:19 -0400 } } 6, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} } } 6, 26 -- To: microscopy-at-microscopy.com } } 6, 26 -- Subject: New SEM donated to our lab- Can you identify the } } accessory? 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: } } text/plain; charset=ISO-8859-1; format=flowed 6, 26 -- } } Content-Transfer-Encoding: 7bit 6, 26 -- Content-Disposition: inline } } ==============================End of - } } Headers============================== } } } } } } } } } } } } _________________________________________________________________ } } Need personalized email and website? Look no further. It's easy with } } Doteasy $0 Web Hosting! Learn more at www.doteasy.com } } } } } } ==============================Original } } Headers============================== } } 28, 29 -- From kenconverse-at-qualityimages.biz Sat Mar 17 11:36:36 2007 } } 28, 29 -- Received: from dpmailmta02.doteasy.com (dpmailmta02.doteasy.com } [65.61.209.90]) } } 28, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id l2HGaaU1026076 } } 28, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sat, 17 Mar 2007 } 11:36:36 -0500 } } 28, 29 -- Received: from qualityimages.biz (unverified [192.168.101.16]) } } 28, 29 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id } 67056218-1814644 } } 28, 29 -- for multiple; Sat, 17 Mar 2007 09:46:58 -0800 } } 28, 29 -- Received: from Ken [76.179.237.214] by qualityimages.biz with } ESMTP } } 28, 29 -- (SMTPD32-8.05) id A90E1B650056; Sat, 17 Mar 2007 09:36:30 } -0700 } } 28, 29 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } } 28, 29 -- To: {kraftpiano-at-gmail.com} , "MSA Listserver" } {Microscopy-at-MSA.Microscopy.Com} } } 28, 29 -- Subject: RE: [Microscopy] New SEM donated to our lab- Can you } identify the accessory? } } 28, 29 -- Date: Sat, 17 Mar 2007 12:36:18 -0400 } } 28, 29 -- Message-ID: {004001c768b2$654b4e90$6401a8c0-at-Ken} } } 28, 29 -- MIME-Version: 1.0 } } 28, 29 -- Content-Type: text/plain; } } 28, 29 -- charset="us-ascii" } } 28, 29 -- X-Priority: 3 (Normal) } } 28, 29 -- X-MSMail-Priority: Normal } } 28, 29 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 } } 28, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 } } 28, 29 -- Thread-Index: AcdoHH2p3h+gQ0uxT2OvjUqyLu6bXwAkPKTg } } 28, 29 -- Importance: Normal } } 28, 29 -- In-Reply-To: {200703162243.l2GMhGZg011302-at-ns.microscopy.com} } } 28, 29 -- X-IMSTrailer: __IMail_7__ } } 28, 29 -- X-IP-stats: Incoming Last 0, First 170, in=3711794, out=0, } spam=0 ip=192.168.101.16 } } 28, 29 -- X-Originating-IP: 192.168.101.16 } } 28, 29 -- Content-Transfer-Encoding: 8bit } } 28, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id l2HGaaU1026076 } } ==============================End of - } Headers============================== } } } } } } } } _________________________________________________________________ } Need personalized email and website? Look no further. It's easy } with Doteasy $0 Web Hosting! Learn more at www.doteasy.com } } } ==============================Original Headers============================== } 19, 30 -- From kenconverse-at-qualityimages.biz Tue Mar 20 10:06:44 2007 } 19, 30 -- Received: from dpmailmta02.doteasy.com (dpmailmta02.doteasy.com [65.61.209.90]) } 19, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2KF6ixi023551 } 19, 30 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 20 Mar 2007 10:06:44 -0500 } 19, 30 -- Received: from qualityimages.biz (unverified [192.168.101.16]) } 19, 30 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id 67624751-1814644 } 19, 30 -- for multiple; Tue, 20 Mar 2007 08:18:45 -0800 } 19, 30 -- Received: from Ken [76.179.237.214] by qualityimages.biz with ESMTP } 19, 30 -- (SMTPD32-8.05) id A87ACAD00CA; Tue, 20 Mar 2007 08:06:34 -0700 } 19, 30 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } 19, 30 -- To: "'Justin Kraft'" {kraftpiano-at-gmail.com} , } 19, 30 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} } 19, 30 -- Subject: RE: [Microscopy] RE: New SEM donated to our lab- Can you identify the accessory? } 19, 30 -- Date: Tue, 20 Mar 2007 11:06:24 -0400 } 19, 30 -- Message-ID: {00ab01c76b01$56367fa0$6401a8c0-at-Ken} } 19, 30 -- MIME-Version: 1.0 } 19, 30 -- Content-Type: text/plain; } 19, 30 -- charset="us-ascii" } 19, 30 -- X-Priority: 3 (Normal) } 19, 30 -- X-MSMail-Priority: Normal } 19, 30 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 } 19, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 } 19, 30 -- Thread-Index: AcdowgMzeUfB1aHNRPeHEVz/wVFmXQCOd2qw } 19, 30 -- Importance: Normal } 19, 30 -- In-Reply-To: {25e2b0d20703171128t18b4ab53saff6cd38c5ed9343-at-mail.gmail.com} } 19, 30 -- X-IMSTrailer: __IMail_7__ } 19, 30 -- X-IP-stats: Incoming Last 0, First 173, in=3747649, out=0, spam=0 ip=192.168.101.16 } 19, 30 -- X-Originating-IP: 192.168.101.16 } 19, 30 -- Content-Transfer-Encoding: 8bit } 19, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2KF6ixi023551 } ==============================End of - Headers============================== }
==============================Original Headers============================== 13, 20 -- From dljones-at-bestweb.net Tue Mar 20 11:45:51 2007 13, 20 -- Received: from vms040pub.verizon.net (vms040pub.verizon.net [206.46.252.40]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2KGjpCY019477 13, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Mar 2007 11:45:51 -0500 13, 20 -- Received: from localhost ([162.84.216.151]) 13, 20 -- by vms040.mailsrvcs.net (Sun Java System Messaging Server 6.2-6.01 (built Apr 13, 20 -- 3 2006)) with ESMTPA id {0JF700KG4NRS4Z90-at-vms040.mailsrvcs.net} for 13, 20 -- Microscopy-at-microscopy.com; Tue, 20 Mar 2007 11:43:11 -0500 (CDT) 13, 20 -- Date: Tue, 20 Mar 2007 12:43:08 -0400 (Eastern Daylight Time) 13, 20 -- From: "David L. Jones" {dljones-at-bestweb.net} 13, 20 -- Subject: Re: [Microscopy] New SEM donated to our lab- Can you identify the 13, 20 -- accessory? 13, 20 -- In-reply-to: {200703201510.l2KFAZLi029845-at-ns.microscopy.com} 13, 20 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 13, 20 -- To: Microscopy-at-microscopy.com 13, 20 -- Cc: kraftpiano-at-gmail.com 13, 20 -- Message-id: {Pine.WNT.4.64.0703201203440.2948-at-H-F1} 13, 20 -- MIME-version: 1.0 13, 20 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 13, 20 -- References: {200703201510.l2KFAZLi029845-at-ns.microscopy.com} ==============================End of - Headers==============================
You make a VERY IMPORTANT point here: "Getting the instrument is the easy part. Getting it up and running consistently well takes a lot of work."
In my SEM course, I always take time to point out to my students that they may be in a situation where someone wants to donate an EM to their school. No matter how well it is working when the donation is accepted, there is no guarantee that it will ever work properly in the new location unless professionals are involved in the packing, shipping and installation. I always recommend having $5-10K available. Hopefully, it will never be needed. My analogy is: "It's like having a baby. The easy part is producing the baby. Caring for it is a labor (hopefully of love)."
JB
} Justin, } Unless they are paying for the shipping, you're going to be out a sizeable } chunk of change just in the shipping costs. If someone doesn't spend at } least a few hours properly packing the system for however it is going to be } shipped (and how it will be shipped makes a difference in how it is packed), } you will receive a large anchor for your boat. Unless you have someone at } your end who is experienced and willing to donate their time for getting } this system back up and running, you probably don't have the budget to get } it running if you don't have the budget to have it packed properly. } } I hate to be so down on this because I currently support an SEM at a high } school because I firmly believe that having the students have the } opportunity to play with the toys that we get to play with in the real world } is a great way to inspire them to get the education they need. However, in } addition to not seeing some major components to the system in your photos } (you also need someone to evalute what's there), I can tell you that I once } packed a system for a customer on a tight budget. I packed it for shipment } by padded van (which is a much less intensive packing job than for a basic } truck), but they decided to just put it on an Estes 18 wheeler despite what } I had told them. By the time it had gone from PA to CA it was trashed. } Penny wise and pound foolish. And they had PAID for that system! } } Unfortunately aquiring a free SEM is an expensive affair unless you can find } someone to do it all properly because THEY are also committed to the } students. It's a sizeable commitment to take on even one SEM pro-bono, but } maybe someone in your area could contact you, if they're interested. } Personally, I would like to see the manufacturers take on a high school } system pro-bono for every x number of field service engineers, but that } probably won't ever happen. (Are you guys paying attention, here?). } } I sincerely hope that someone DOES contact you and I also hope that my doom } and gloom is off the mark. } } Best wishes } Ken Converse } Owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } } -----Original Message----- } X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com] } Sent: Saturday, March 17, 2007 2:28 PM } To: kenconverse-at-qualityimages.biz } Subject: Re: [Microscopy] RE: New SEM donated to our lab- Can you identify } the accessory? } } } It's in New Jersey right now, and unfortunately we don't have the budget to } send someone up there to pack it properly. The company who is donating it } will not spend more than a few minutes prepping it (They aren't making money } off of it, and they are electronics recyclers, so they just see it as a pile } of metal) so I need to make instructions as simple and clear as possible, } without having too much for them to do, or they may just decide to Ebay the } whole thing. } } --Justin. } } On 3/17/07, kenconverse-at-qualityimages.biz {kenconverse-at-qualityimages.biz} } wrote: } } } } } } } } ---------------------------------------------------------------------- } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Justin, } } I believe what you have is a JXA-840 (microprobe) with 2 wavelength } } spectrometers and a light microscope for establishing the proper WD } } for using those spectrometers. The Box on top is an ion pump so that } } the system can be used with a LaB6 cathode, if you choose (and have } } received the correct wehnelt cap). } } } } Whether it is an 840 or 840A could be told by a look at the } } electronics console, but you don't have any pictures of that or the } } electronics rack that should be present for the spectometers. I do } } see the power supply console in a couple of pictures. Does it include } } the rotary pump and possibly compressor? } } } } As far as prepping for shipping, the first thing that jumps out at me } } is that the table isn't bolted down! At a minimum you need to get 4 } } M16x2.00x40mm bolts and 16mm washers to secure the table. You also } } need to remove the magnet from the ion pump. Actually, most of the } } column should be removed and packed separately unless you're planning } } to move it only a few miles at very low speed. I also suspect that } } the WDS units and light microscope should be removed. All 3 are } } complex and fragile. } } } } Is it diffusion pumped or turbo pumped? If it's turbo pumped, the } } pump must be removed before shipping. Also the high voltage oil tank } } in the electronics console must be removed and the circuit board on } } its side protected from damage. } } } } This is going to the W. Palm Beach area of Florida. Where is it } } currently located? I would highly recommend sending someone to pack } } it properly. How is it going to be shipped? If it's going by truck, } } it must be "padded van" unless you're going to have a lot more of it } } disassembled, crated and put on pallets. } } } } With more info, I might be of more help. } } } } Ken Converse } } owner } } } } QUALITY IMAGES } } Servicing Scanning Electron Microscopes } } Since 1981 } } 474 So. Bridgton Rd. } } Bridgton, ME 04009 } } 207-647-4348 } } Fax 207-647-2688 } } kenconverse-at-qualityimages.biz } } qualityimages.biz } } } } } } } } -----Original Message----- } } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] } } Sent: Friday, March 16, 2007 6:43 PM } } To: kenconverse-at-qualityimages.biz } } Subject: [Microscopy] New SEM donated to our lab- Can you identify the } } accessory? } } } } } } } } } } } } ---------------------------------------------------------------------- } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Hello all, } } } } We have a new SEM on the way to us, it's a JEOL JSM-840. All I have } } at this point are a couple of pictures, and I am a little flummoxed } } about the accessories that are added on this instrument. I recognize } } the secondary electron detector, and I believe that there is a } } backscatter detector on it, but I'm not sure what the other } } accessories are. Also, the photos that I have seen of the basic 840s } } don't include a box sitting on top of the column, but this one has it. } } It's a silver-ish box with black sides, and is barely visible in one } } of the photos, but it is there, at a slight angle. (Which leads me to } } the next question- how should it be secured for } } shipping?) } } } } The whole microscope is being packed by people who don't necessarily } } know how to package it. Do any of you have specific suggestions of } } what they could do to prevent damage in shipping? } } } } Here is the URL to the photos of the microscope (Hosted on my personal } } page which has not been updated in a long time...) } } http://www.jkraft.net/JEOL/ } } } } Thanks, } } } } Justin A. Kraft } } Director } } Center for Inquiry-Based Science Education } } } } ==============================Original } } Headers============================== } } 6, 26 -- From kraftpiano-at-gmail.com Fri Mar 16 17:40:22 2007 } } 6, 26 -- Received: from ik-out-1112.google.com (ik-out-1112.google.com } } [66.249.90.177]) } } 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id } } l2GMeL9w006622 } } 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 17:40:22 } } -0500 } } 6, 26 -- Received: by ik-out-1112.google.com with SMTP id b32so541347ika } } 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 } 15:40:20 } } -0700 (PDT) } } 6, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; } } 6, 26 -- d=gmail.com; s=beta; } } 6, 26 -- } } } h=domainkey-signature:received:received:message-id:date:from:to:subject:mime } } -version:content-type:content-transfer-encoding:content-disposition; } } 6, 26 -- } } } b=s1DejygYHazI0gOXcGdlH3aFhc31xI8o6mt+j5GOcTzrxtJPW9PLWzsfWcdgsZQjbUfGwio8NU } } weB8pHDxwgatI0zP5kv+TIfnL1G2A6U75mJ90Wu2/xog0gZFV7g5sBV3vJe5jiayVV4dGS } } weB8pHDxwgatI0zP5kv+kp74tv } } q2VQyDtZdd3Icvrv93WZs= } } 6, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } } 6, 26 -- d=gmail.com; s=beta; } } 6, 26 -- } } h=received:message-id:date:from:to:subject:mime-version:content-type:c } } ontent } } -transfer-encoding:content-disposition; } } 6, 26 -- } } } b=pR3ppcp1LBgkdhNL2mxb8fxkll/KXvwAFHADQW/u6FINK4J15EsY55ex1+B2+g0JNvff5T3gaa } } YDWFX6PzBDM720L+slCXtxDVdzFMBAPEocfGL+xIvvPiSQg1ACUQ8+ujyW+Q/J0di/cxKy } } YDWFX6PzBDM720L+slCXtxDVdzFMBAPEocfGL+xIvvPiSQg1ACUQ8+ujyW+HEXk7+ } } jE105VLOBzCHBFkml2E+0= } } 6, 26 -- Received: by 10.114.171.1 with SMTP id } t1mr918673wae.1174084819773; } } 6, 26 -- Fri, 16 Mar 2007 15:40:19 -0700 (PDT) } } 6, 26 -- Received: by 10.114.79.12 with HTTP; Fri, 16 Mar 2007 } } 15:40:19 -0700 (PDT) 6, 26 -- Message-ID: } } {25e2b0d20703161540k140001d5k6170427b9d645d6b-at-mail.gmail.com} } } 6, 26 -- Date: Fri, 16 Mar 2007 18:40:19 -0400 } } 6, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} } } 6, 26 -- To: microscopy-at-microscopy.com } } 6, 26 -- Subject: New SEM donated to our lab- Can you identify the } } accessory? 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: } } text/plain; charset=ISO-8859-1; format=flowed 6, 26 -- } } Content-Transfer-Encoding: 7bit 6, 26 -- Content-Disposition: inline } } ==============================End of - } } Headers============================== } } } } } } } } } } } } _________________________________________________________________ } } Need personalized email and website? Look no further. It's easy with } } Doteasy $0 Web Hosting! Learn more at www.doteasy.com } } } } } } ==============================Original } } Headers============================== } } 28, 29 -- From kenconverse-at-qualityimages.biz Sat Mar 17 11:36:36 2007 } } 28, 29 -- Received: from dpmailmta02.doteasy.com (dpmailmta02.doteasy.com } [65.61.209.90]) } } 28, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id l2HGaaU1026076 } } 28, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sat, 17 Mar 2007 } 11:36:36 -0500 } } 28, 29 -- Received: from qualityimages.biz (unverified [192.168.101.16]) } } 28, 29 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id } 67056218-1814644 } } 28, 29 -- for multiple; Sat, 17 Mar 2007 09:46:58 -0800 } } 28, 29 -- Received: from Ken [76.179.237.214] by qualityimages.biz with } ESMTP } } 28, 29 -- (SMTPD32-8.05) id A90E1B650056; Sat, 17 Mar 2007 09:36:30 } -0700 } } 28, 29 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } } 28, 29 -- To: {kraftpiano-at-gmail.com} , "MSA Listserver" } {Microscopy-at-MSA.Microscopy.Com} } } 28, 29 -- Subject: RE: [Microscopy] New SEM donated to our lab- Can you } identify the accessory? } } 28, 29 -- Date: Sat, 17 Mar 2007 12:36:18 -0400 } } 28, 29 -- Message-ID: {004001c768b2$654b4e90$6401a8c0-at-Ken} } } 28, 29 -- MIME-Version: 1.0 } } 28, 29 -- Content-Type: text/plain; } } 28, 29 -- charset="us-ascii" } } 28, 29 -- X-Priority: 3 (Normal) } } 28, 29 -- X-MSMail-Priority: Normal } } 28, 29 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 } } 28, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 } } 28, 29 -- Thread-Index: AcdoHH2p3h+gQ0uxT2OvjUqyLu6bXwAkPKTg } } 28, 29 -- Importance: Normal } } 28, 29 -- In-Reply-To: {200703162243.l2GMhGZg011302-at-ns.microscopy.com} } } 28, 29 -- X-IMSTrailer: __IMail_7__ } } 28, 29 -- X-IP-stats: Incoming Last 0, First 170, in=3711794, out=0, } spam=0 ip=192.168.101.16 } } 28, 29 -- X-Originating-IP: 192.168.101.16 } } 28, 29 -- Content-Transfer-Encoding: 8bit } } 28, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id l2HGaaU1026076 } } ==============================End of - } Headers============================== } } } } } } } } _________________________________________________________________ } Need personalized email and website? 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Learn more at www.doteasy.com } } } ==============================Original Headers============================== } 19, 30 -- From kenconverse-at-qualityimages.biz Tue Mar 20 10:06:44 2007 } 19, 30 -- Received: from dpmailmta02.doteasy.com } (dpmailmta02.doteasy.com [65.61.209.90]) } 19, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l2KF6ixi023551 } 19, 30 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 20 Mar 2007 } 10:06:44 -0500 } 19, 30 -- Received: from qualityimages.biz (unverified [192.168.101.16]) } 19, 30 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id 67624751-1814644 } 19, 30 -- for multiple; Tue, 20 Mar 2007 08:18:45 -0800 } 19, 30 -- Received: from Ken [76.179.237.214] by qualityimages.biz with ESMTP } 19, 30 -- (SMTPD32-8.05) id A87ACAD00CA; Tue, 20 Mar 2007 08:06:34 -0700 } 19, 30 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } 19, 30 -- To: "'Justin Kraft'" {kraftpiano-at-gmail.com} , } 19, 30 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} } 19, 30 -- Subject: RE: [Microscopy] RE: New SEM donated to our lab- } Can you identify the accessory? } 19, 30 -- Date: Tue, 20 Mar 2007 11:06:24 -0400 } 19, 30 -- Message-ID: {00ab01c76b01$56367fa0$6401a8c0-at-Ken} } 19, 30 -- MIME-Version: 1.0 } 19, 30 -- Content-Type: text/plain; } 19, 30 -- charset="us-ascii" } 19, 30 -- X-Priority: 3 (Normal) } 19, 30 -- X-MSMail-Priority: Normal } 19, 30 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 } 19, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 } 19, 30 -- Thread-Index: AcdowgMzeUfB1aHNRPeHEVz/wVFmXQCOd2qw } 19, 30 -- Importance: Normal } 19, 30 -- In-Reply-To: } {25e2b0d20703171128t18b4ab53saff6cd38c5ed9343-at-mail.gmail.com} } 19, 30 -- X-IMSTrailer: __IMail_7__ } 19, 30 -- X-IP-stats: Incoming Last 0, First 173, in=3747649, out=0, } spam=0 ip=192.168.101.16 } 19, 30 -- X-Originating-IP: 192.168.101.16 } 19, 30 -- Content-Transfer-Encoding: 8bit } 19, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id l2KF6ixi023551 } ==============================End of - Headers==============================
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==============================Original Headers============================== 8, 21 -- From bozzola-at-siu.edu Tue Mar 20 14:01:00 2007 8, 21 -- Received: from abbmta2.siu.edu (abbmta2.siu.edu [131.230.254.206]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2KJ0xQa012045 8, 21 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 20 Mar 2007 14:01:00 -0500 8, 21 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) 8, 21 -- by abbmta2.siu.edu (Switch-3.1.11/Switch-3.1.11) with ESMTP id l2KJ0jnX013016 8, 21 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 20 Mar 2007 14:00:46 -0500 (CDT) 8, 21 -- Mime-Version: 1.0 8, 21 -- X-Sender: bozzola-at-saluki-mail.siu.edu 8, 21 -- Message-Id: {p0611040bc225ddd7c662-at-[131.230.177.142]} 8, 21 -- In-Reply-To: {200703201507.l2KF7sPB025219-at-ns.microscopy.com} 8, 21 -- References: {200703201507.l2KF7sPB025219-at-ns.microscopy.com} 8, 21 -- Date: Tue, 20 Mar 2007 14:00:44 -0500 8, 21 -- To: Microscopy-at-msa.microscopy.com 8, 21 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 8, 21 -- Subject: Re: [Microscopy] New SEM donated to our lab- Can you identify 8, 21 -- the accessory? 8, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 8, 21 -- X-Spam-Score: 0.00% 8, 21 -- X-MASF: 0.00% 8, 21 -- X-Whitelist: 0.00% ==============================End of - Headers==============================
Ernst Leitz GmbH is now called 'Leica'. Leica is now three companies: Leica Camera AG, which produces cameras; Leica Geosystems AG which produces geosurvey equipment; and Leica Microsystems GmbH, which produces microscopes.
Leica Microsystems GmbH is the owner of the Leica brand, and grants licenses to Leica Camera AG and Leica Geosystems. I've not seen any new microscopes under the Leitz brand recently, they all seem to now go under the Leica name, although there are many modernish equivalents of your Laborlux about, e.g.
http://www.saulresearch.com/photomicroscopy.htm
Which seem to be from the 1980s.
Yours is no doubt an older variety of the brand, and they kept the Laborlux name for new models. e.g.
Microscopes can come with a lot of optional accessories for illumination, contrast enhancement etc.. so yours may or may not have these (and this affects value as all parts are rather expensive on a microscope and add up). Even the magnifying objectives are bought separately (as they are expensive and you buy those most suited to your needs and pocket). www.leica.com [Microsystems] should be able to provide you with some details, like the year of manufacture, and perhaps even a pamphlet/brochure scan. They no doubt have an archivist/museum but possibly not much free time pass on info.
If you want the microscope cleaned/serviced there will be local microscope independents who specialise in this sort of thing - these undercut the manufacturers who are very expensive (£300+ just to arrive). However all are geared towards laboratories who have deeper pockets. As mentioned by Caroline, a local microscope society will be a far better option, as these enthusiasts are keen to help and have a sounder knowledge of old (cheap) kit than a professional microscopist. All our systems are over £10,000 and many mainstay systems are £250,000 (e.g. 'confocal' microscopes with multiple lasers as the light source and all controlled by PCs). Thus we aren't a lot of help in this case, as few of us will have held or used a microscope like yours (it's even before my time), although we may have seen similar in the lab cupboard as a youth.
If the microscope is serviceable you may just need to clean the eyepieces and objectives if dirty, and perhaps lubricate the mechanical parts (those microscope society chaps can advise here). This needs some care and specialised equipment/fluids though and I can advise if you want to clean the parts yourself for personal use. Otherwise I would sell it 'as is' - microscopes are as often bought as much for display as a working scientific instrument, although yours has the potential to be used for fun amateur study if clean/working (and similar ones are still used professionally in the field rather than in the microscope lab). If sold 'as is', the buyer then takes responsibility for cleaning (and any damage that results).
If you want to sell, Leitz is a quality brand that will attract a premium. There are many examples of Leitz microscopes second hand on the web and on ebay. Have a look and get a feel for the going price (dealers may make extortionate mark-ups though). Also track a few Leitz microscopes through ebay.com (there's no rush to sell). I would expect the microscope to be easily valued at well over £200, and rather more if it is in excellent serviceable condition and is complete. It will have probably cost several thousand pounds new in today's money (or £100 in 1950's money). Antique high quality microscopes (older than 1900, attract a serious collectors attention and a premium, but a 1950's model is often a little less valuable as it has less pretty brass and is just a bit large to put in a display cabinet. But like most things, it just depends on it's condition and how much someone is prepared to pay for it on the day.
Regards
Keith
--------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Institute of Ophthalmology UCL, London EC1V 9EL
There are a few sites on the web interested in old microscopes, so have a search. I found a few:
There is a Leitz Laborlux 12 POL Microscope Brouchure and a more modern Labolux microscope on ebay.com at the moment that looks quite decent (no takers for the $1,300 price tag yet).
-----Original Message----- X-from: paigelassen-at-yahoo.com [mailto:paigelassen-at-yahoo.com] Sent: 20 March 2007 02:44 To: keith.morris-at-ucl.ac.uk
This Question was submitted to Ask-A-Microscopist by (paigelassen-at-yahoo.com)
from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, March 9, 2007 at 10:09:46 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both paigelassen-at-yahoo.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: Good morning. I have inherited a microscope and considering selling it. My dad acquired it from a pathologist for medical school back in 1960. If you could provide information about the microscope or direct me to a service that appraises microscopes, it would be much appreciated. It is an Ernst Leitz GmbH/Wetzlar Laborlux. Serial #(?) is 519 465. Has all original components, slides, box, and papers written in German. Thank you in advance for any recommendations.
You all have to hire the guys that moved the microscope on the TV show, "Crossing Jordan".
In the story line, they had moved the EM out of the lab for cost cutting and had it in the basement. When they needed it, the boss told them to "go get my microscope" and they had it up and running in less than an hour.
Well, I thought it was funny!
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: Tuesday, March 20, 2007 11:06 AM To: Walck-at-SouthBayTech.com
Ken,
You make a VERY IMPORTANT point here: "Getting the instrument is the easy part. Getting it up and running consistently well takes a lot of work."
In my SEM course, I always take time to point out to my students that they may be in a situation where someone wants to donate an EM to their school. No matter how well it is working when the donation is accepted, there is no guarantee that it will ever work properly in the new location unless professionals are involved in the packing, shipping and installation. I always recommend having $5-10K available. Hopefully, it will never be needed. My analogy is: "It's like having a baby. The easy part is producing the baby. Caring for it is a labor (hopefully of love)."
JB
} Justin, } Unless they are paying for the shipping, you're going to be out a } sizeable chunk of change just in the shipping costs. If someone } doesn't spend at least a few hours properly packing the system for } however it is going to be shipped (and how it will be shipped makes a } difference in how it is packed), you will receive a large anchor for } your boat. Unless you have someone at your end who is experienced and } willing to donate their time for getting this system back up and } running, you probably don't have the budget to get it running if you don't have the budget to have it packed properly. } } I hate to be so down on this because I currently support an SEM at a } high school because I firmly believe that having the students have the } opportunity to play with the toys that we get to play with in the real } world is a great way to inspire them to get the education they need. } However, in addition to not seeing some major components to the system } in your photos (you also need someone to evalute what's there), I can } tell you that I once packed a system for a customer on a tight budget. } I packed it for shipment by padded van (which is a much less intensive } packing job than for a basic truck), but they decided to just put it on } an Estes 18 wheeler despite what I had told them. By the time it had gone from PA to CA it was trashed. } Penny wise and pound foolish. And they had PAID for that system! } } Unfortunately aquiring a free SEM is an expensive affair unless you can } find someone to do it all properly because THEY are also committed to } the students. It's a sizeable commitment to take on even one SEM } pro-bono, but maybe someone in your area could contact you, if they're interested. } Personally, I would like to see the manufacturers take on a high school } system pro-bono for every x number of field service engineers, but that } probably won't ever happen. (Are you guys paying attention, here?). } } I sincerely hope that someone DOES contact you and I also hope that my } doom and gloom is off the mark. } } Best wishes } Ken Converse } Owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } } -----Original Message----- } X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com] } Sent: Saturday, March 17, 2007 2:28 PM } To: kenconverse-at-qualityimages.biz } Subject: Re: [Microscopy] RE: New SEM donated to our lab- Can you } identify the accessory? } } } It's in New Jersey right now, and unfortunately we don't have the } budget to send someone up there to pack it properly. The company who } is donating it will not spend more than a few minutes prepping it (They } aren't making money off of it, and they are electronics recyclers, so } they just see it as a pile of metal) so I need to make instructions as } simple and clear as possible, without having too much for them to do, } or they may just decide to Ebay the whole thing. } } --Justin. } } On 3/17/07, kenconverse-at-qualityimages.biz } {kenconverse-at-qualityimages.biz} } wrote: } } } } } } } } } } --------------------------------------------------------------------- } } - } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ----------------------------------------------------------------------- } ----- } } } } Justin, } } I believe what you have is a JXA-840 (microprobe) with 2 wavelength } } spectrometers and a light microscope for establishing the proper WD } } for using those spectrometers. The Box on top is an ion pump so that } } the system can be used with a LaB6 cathode, if you choose (and have } } received the correct wehnelt cap). } } } } Whether it is an 840 or 840A could be told by a look at the } } electronics console, but you don't have any pictures of that or the } } electronics rack that should be present for the spectometers. I do } } see the power supply console in a couple of pictures. Does it } } include the rotary pump and possibly compressor? } } } } As far as prepping for shipping, the first thing that jumps out at } } me is that the table isn't bolted down! At a minimum you need to } } get 4 M16x2.00x40mm bolts and 16mm washers to secure the table. You } } also need to remove the magnet from the ion pump. Actually, most of } } the column should be removed and packed separately unless you're } } planning to move it only a few miles at very low speed. I also } } suspect that the WDS units and light microscope should be removed. } } All 3 are complex and fragile. } } } } Is it diffusion pumped or turbo pumped? If it's turbo pumped, the } } pump must be removed before shipping. Also the high voltage oil tank } } in the electronics console must be removed and the circuit board on } } its side protected from damage. } } } } This is going to the W. Palm Beach area of Florida. Where is it } } currently located? I would highly recommend sending someone to pack } } it properly. How is it going to be shipped? If it's going by truck, } } it must be "padded van" unless you're going to have a lot more of it } } disassembled, crated and put on pallets. } } } } With more info, I might be of more help. } } } } Ken Converse } } owner } } } } QUALITY IMAGES } } Servicing Scanning Electron Microscopes Since 1981 } } 474 So. Bridgton Rd. } } Bridgton, ME 04009 } } 207-647-4348 } } Fax 207-647-2688 } } kenconverse-at-qualityimages.biz } } qualityimages.biz } } } } } } } } -----Original Message----- } } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] } } Sent: Friday, March 16, 2007 6:43 PM } } To: kenconverse-at-qualityimages.biz } } Subject: [Microscopy] New SEM donated to our lab- Can you identify the } } accessory? } } } } } } } } } } } } ---------------------------------------------------------------------- } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } --------------------------------------------------------------------------- - } } } } Hello all, } } } } We have a new SEM on the way to us, it's a JEOL JSM-840. All I have } } at this point are a couple of pictures, and I am a little flummoxed } } about the accessories that are added on this instrument. I recognize } } the secondary electron detector, and I believe that there is a } } backscatter detector on it, but I'm not sure what the other } } accessories are. Also, the photos that I have seen of the basic 840s } } don't include a box sitting on top of the column, but this one has it. } } It's a silver-ish box with black sides, and is barely visible in one } } of the photos, but it is there, at a slight angle. (Which leads me to } } the next question- how should it be secured for } } shipping?) } } } } The whole microscope is being packed by people who don't necessarily } } know how to package it. Do any of you have specific suggestions of } } what they could do to prevent damage in shipping? } } } } Here is the URL to the photos of the microscope (Hosted on my personal } } page which has not been updated in a long time...) } } http://www.jkraft.net/JEOL/ } } } } Thanks, } } } } Justin A. Kraft } } Director } } Center for Inquiry-Based Science Education } } } } ==============================Original } } Headers============================== } } 6, 26 -- From kraftpiano-at-gmail.com Fri Mar 16 17:40:22 2007 } } 6, 26 -- Received: from ik-out-1112.google.com (ik-out-1112.google.com } } [66.249.90.177]) } } 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id } } l2GMeL9w006622 } } 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 17:40:22 } } -0500 } } 6, 26 -- Received: by ik-out-1112.google.com with SMTP id b32so541347ika } } 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 } 15:40:20 } } -0700 (PDT) } } 6, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; } } 6, 26 -- d=gmail.com; s=beta; } } 6, 26 -- } } } h=domainkey-signature:received:received:message-id:date:from:to:subject:mim e } } -version:content-type:content-transfer-encoding:content-disposition; } } 6, 26 -- } } } b=s1DejygYHazI0gOXcGdlH3aFhc31xI8o6mt+j5GOcTzrxtJPW9PLWzsfWcdgsZQjbUfGwio8N U } } weB8pHDxwgatI0zP5kv+TIfnL1G2A6U75mJ90Wu2/xog0gZFV7g5sBV3vJe5jiayVV4dGS } } weB8pHDxwgatI0zP5kv+kp74tv } } q2VQyDtZdd3Icvrv93WZs= } } 6, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } } 6, 26 -- d=gmail.com; s=beta; } } 6, 26 -- } } h=received:message-id:date:from:to:subject:mime-version:content-type:c } } ontent } } -transfer-encoding:content-disposition; } } 6, 26 -- } } } b=pR3ppcp1LBgkdhNL2mxb8fxkll/KXvwAFHADQW/u6FINK4J15EsY55ex1+B2+g0JNvff5T3ga a } } YDWFX6PzBDM720L+slCXtxDVdzFMBAPEocfGL+xIvvPiSQg1ACUQ8+ujyW+Q/J0di/cxKy } } YDWFX6PzBDM720L+slCXtxDVdzFMBAPEocfGL+xIvvPiSQg1ACUQ8+ujyW+HEXk7+ } } jE105VLOBzCHBFkml2E+0= } } 6, 26 -- Received: by 10.114.171.1 with SMTP id } t1mr918673wae.1174084819773; } } 6, 26 -- Fri, 16 Mar 2007 15:40:19 -0700 (PDT) } } 6, 26 -- Received: by 10.114.79.12 with HTTP; Fri, 16 Mar 2007 } } 15:40:19 -0700 (PDT) 6, 26 -- Message-ID: } } {25e2b0d20703161540k140001d5k6170427b9d645d6b-at-mail.gmail.com} } } 6, 26 -- Date: Fri, 16 Mar 2007 18:40:19 -0400 } } 6, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} } } 6, 26 -- To: microscopy-at-microscopy.com } } 6, 26 -- Subject: New SEM donated to our lab- Can you identify the } } accessory? 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: } } text/plain; charset=ISO-8859-1; format=flowed 6, 26 -- } } Content-Transfer-Encoding: 7bit 6, 26 -- Content-Disposition: inline } } ==============================End of - } } Headers============================== } } } } } } } } } } } } _________________________________________________________________ } } Need personalized email and website? 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Learn more at www.doteasy.com } } } } } } ==============================Original } } Headers============================== } } 28, 29 -- From kenconverse-at-qualityimages.biz Sat Mar 17 11:36:36 2007 } } 28, 29 -- Received: from dpmailmta02.doteasy.com (dpmailmta02.doteasy.com } [65.61.209.90]) } } 28, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id l2HGaaU1026076 } } 28, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sat, 17 Mar 2007 } 11:36:36 -0500 } } 28, 29 -- Received: from qualityimages.biz (unverified [192.168.101.16]) } } 28, 29 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id } 67056218-1814644 } } 28, 29 -- for multiple; Sat, 17 Mar 2007 09:46:58 -0800 } } 28, 29 -- Received: from Ken [76.179.237.214] by qualityimages.biz with } ESMTP } } 28, 29 -- (SMTPD32-8.05) id A90E1B650056; Sat, 17 Mar 2007 09:36:30 } -0700 } } 28, 29 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } } 28, 29 -- To: {kraftpiano-at-gmail.com} , "MSA Listserver" } {Microscopy-at-MSA.Microscopy.Com} } } 28, 29 -- Subject: RE: [Microscopy] New SEM donated to our lab- Can you } identify the accessory? } } 28, 29 -- Date: Sat, 17 Mar 2007 12:36:18 -0400 } } 28, 29 -- Message-ID: {004001c768b2$654b4e90$6401a8c0-at-Ken} } } 28, 29 -- MIME-Version: 1.0 } } 28, 29 -- Content-Type: text/plain; } } 28, 29 -- charset="us-ascii" } } 28, 29 -- X-Priority: 3 (Normal) } } 28, 29 -- X-MSMail-Priority: Normal } } 28, 29 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 } } 28, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 } } 28, 29 -- Thread-Index: AcdoHH2p3h+gQ0uxT2OvjUqyLu6bXwAkPKTg } } 28, 29 -- Importance: Normal } } 28, 29 -- In-Reply-To: {200703162243.l2GMhGZg011302-at-ns.microscopy.com} } } 28, 29 -- X-IMSTrailer: __IMail_7__ } } 28, 29 -- X-IP-stats: Incoming Last 0, First 170, in=3711794, out=0, } spam=0 ip=192.168.101.16 } } 28, 29 -- X-Originating-IP: 192.168.101.16 } } 28, 29 -- Content-Transfer-Encoding: 8bit } } 28, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id l2HGaaU1026076 } } ==============================End of - } Headers============================== } } } } } } } } _________________________________________________________________ } Need personalized email and website? 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Learn more at www.doteasy.com } } } ==============================Original Headers============================== } 19, 30 -- From kenconverse-at-qualityimages.biz Tue Mar 20 10:06:44 2007 } 19, 30 -- Received: from dpmailmta02.doteasy.com } (dpmailmta02.doteasy.com [65.61.209.90]) } 19, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l2KF6ixi023551 } 19, 30 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 20 Mar 2007 } 10:06:44 -0500 } 19, 30 -- Received: from qualityimages.biz (unverified [192.168.101.16]) } 19, 30 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id 67624751-1814644 } 19, 30 -- for multiple; Tue, 20 Mar 2007 08:18:45 -0800 } 19, 30 -- Received: from Ken [76.179.237.214] by qualityimages.biz with ESMTP } 19, 30 -- (SMTPD32-8.05) id A87ACAD00CA; Tue, 20 Mar 2007 08:06:34 -0700 } 19, 30 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } 19, 30 -- To: "'Justin Kraft'" {kraftpiano-at-gmail.com} , } 19, 30 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} } 19, 30 -- Subject: RE: [Microscopy] RE: New SEM donated to our lab- } Can you identify the accessory? } 19, 30 -- Date: Tue, 20 Mar 2007 11:06:24 -0400 } 19, 30 -- Message-ID: {00ab01c76b01$56367fa0$6401a8c0-at-Ken} } 19, 30 -- MIME-Version: 1.0 } 19, 30 -- Content-Type: text/plain; } 19, 30 -- charset="us-ascii" } 19, 30 -- X-Priority: 3 (Normal) } 19, 30 -- X-MSMail-Priority: Normal } 19, 30 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 } 19, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 } 19, 30 -- Thread-Index: AcdowgMzeUfB1aHNRPeHEVz/wVFmXQCOd2qw } 19, 30 -- Importance: Normal } 19, 30 -- In-Reply-To: } {25e2b0d20703171128t18b4ab53saff6cd38c5ed9343-at-mail.gmail.com} } 19, 30 -- X-IMSTrailer: __IMail_7__ } 19, 30 -- X-IP-stats: Incoming Last 0, First 173, in=3747649, out=0, } spam=0 ip=192.168.101.16 } 19, 30 -- X-Originating-IP: 192.168.101.16 } 19, 30 -- Content-Transfer-Encoding: 8bit } 19, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id l2KF6ixi023551 } ==============================End of - Headers==============================
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==============================Original Headers============================== 8, 21 -- From bozzola-at-siu.edu Tue Mar 20 14:01:00 2007 8, 21 -- Received: from abbmta2.siu.edu (abbmta2.siu.edu [131.230.254.206]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2KJ0xQa012045 8, 21 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 20 Mar 2007 14:01:00 -0500 8, 21 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) 8, 21 -- by abbmta2.siu.edu (Switch-3.1.11/Switch-3.1.11) with ESMTP id l2KJ0jnX013016 8, 21 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 20 Mar 2007 14:00:46 -0500 (CDT) 8, 21 -- Mime-Version: 1.0 8, 21 -- X-Sender: bozzola-at-saluki-mail.siu.edu 8, 21 -- Message-Id: {p0611040bc225ddd7c662-at-[131.230.177.142]} 8, 21 -- In-Reply-To: {200703201507.l2KF7sPB025219-at-ns.microscopy.com} 8, 21 -- References: {200703201507.l2KF7sPB025219-at-ns.microscopy.com} 8, 21 -- Date: Tue, 20 Mar 2007 14:00:44 -0500 8, 21 -- To: Microscopy-at-msa.microscopy.com 8, 21 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 8, 21 -- Subject: Re: [Microscopy] New SEM donated to our lab- Can you identify 8, 21 -- the accessory? 8, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 8, 21 -- X-Spam-Score: 0.00% 8, 21 -- X-MASF: 0.00% 8, 21 -- X-Whitelist: 0.00% ==============================End of - Headers==============================
==============================Original Headers============================== 19, 22 -- From walck-at-southbaytech.com Tue Mar 20 15:22:30 2007 19, 22 -- Received: from flpi102.sbcis.sbc.com (flpi102.sbcis.sbc.com [207.115.20.71]) 19, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2KKMTSh004709 19, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Mar 2007 15:22:30 -0500 19, 22 -- X-ORBL: [64.169.217.123] 19, 22 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) 19, 22 -- by flpi102.sbcis.sbc.com (8.13.8 out.dk.spool/8.13.8) with ESMTP id l2KKMIAn018852; 19, 22 -- Tue, 20 Mar 2007 13:22:19 -0700 19, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} 19, 22 -- To: {bozzola-at-siu.edu} 19, 22 -- Cc: {Microscopy-at-microscopy.com} 19, 22 -- Subject: RE: [Microscopy] Re: New SEM donated to our lab- Moving a microscope -For Fun 19, 22 -- Date: Tue, 20 Mar 2007 13:21:06 -0800 19, 22 -- Message-ID: {004d01c76b35$acc02220$7801a8c0-at-dynamicbl8uno3} 19, 22 -- MIME-Version: 1.0 19, 22 -- Content-Type: text/plain; 19, 22 -- charset="us-ascii" 19, 22 -- Content-Transfer-Encoding: 7bit 19, 22 -- X-Mailer: Microsoft Office Outlook 11 19, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 19, 22 -- In-Reply-To: {200703201905.l2KJ5dII017968-at-ns.microscopy.com} 19, 22 -- Thread-Index: AcdrIsAkh+8+WaVTTsebGWA8my5x1gAEl67A ==============================End of - Headers==============================
I was very amused by that quip as well. Of course, I was also amused that they kept calling it an "Electron Scanner Microscope."
Oh, well.
Thanks for all of the replies, guys. Unfortunately, I have little to no control over the packing. They will take basic suggestions, and do some basic things, but generally they are in a different line of business- electronics recycling. They were put in the position of having it and not knowing how to sell it, so they ended up giving it to me. The way I figure, I'll have them batten it down as best as they are willing to do, and hope. (Logically, since according to them it was taken out some 50 miles or so away from where they are, and they took it out, I figure that any damage that will be done to it has already been done.)
They're splitting the shipping costs with us as well, so the total out of pocket expense for us is $300.
Worst case scenario I end up with a bunch of broken parts, but the basis for putting together a working instrument over the next several years. My degree is in physics, and so I will at least enjoy the challenge of figuring it out from a circuits and wires point of view.
They don't have any manuals or anything- all I'm getting is the column, the display console, and the power supply box. They sold the pump box (Probably because that was something they recognized) on Ebay, so my first challenge will be getting a pump up and running. I might steal the one off of the ISI SX-40A I have at home for a little while. After that, there is an air conditioning service vacuum sales representative who is willing to donate one of his pumps and some gauges and such.
When the SEM gets here, I'll post regular updates (If you're interested) on our progress. One of my fortes is documentation, so I will at least document the whole process on a website.
Doom and gloom predictions aside now, let's all hold our breaths and cross our collective fingers and toes.
--Justin.
On 3/20/07, walck-at-southbaytech.com {walck-at-southbaytech.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } You all have to hire the guys that moved the microscope on the TV show, } "Crossing Jordan". } } In the story line, they had moved the EM out of the lab for cost cutting and } had it in the basement. When they needed it, the boss told them to "go get } my microscope" and they had it up and running in less than an hour. } } Well, I thought it was funny! } } } -Scott } } Scott D. Walck, Ph.D. } Technical Director } South Bay Technology, Inc. } 1120 Via Callejon } San Clemente, CA 92673 } } US Toll Free: 1-800-728-2233 } Tel: (949) 492-2600 } Fax: (949) 492-1499 } } www.southbaytech.com } walck-at-southbaytech.com } } -----Original Message----- } X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] } Sent: Tuesday, March 20, 2007 11:06 AM } To: Walck-at-SouthBayTech.com } Subject: [Microscopy] Re: New SEM donated to our lab- Can you identify } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Ken, } } You make a VERY IMPORTANT point here: "Getting the instrument is the easy } part. Getting it up and running consistently well takes a lot of work." } } In my SEM course, I always take time to point out to my students that they } may be in a situation where someone wants to donate an EM to their school. } No matter how well it is working when the donation is accepted, there is no } guarantee that it will ever work properly in the new location unless } professionals are involved in the packing, shipping and installation. I } always recommend having $5-10K available. Hopefully, it will never be } needed. My analogy is: "It's like having a baby. The easy part is producing } the baby. Caring for it is a labor (hopefully of love)." } } JB } } } } Justin, } } Unless they are paying for the shipping, you're going to be out a } } sizeable chunk of change just in the shipping costs. If someone } } doesn't spend at least a few hours properly packing the system for } } however it is going to be shipped (and how it will be shipped makes a } } difference in how it is packed), you will receive a large anchor for } } your boat. Unless you have someone at your end who is experienced and } } willing to donate their time for getting this system back up and } } running, you probably don't have the budget to get it running if you don't } have the budget to have it packed properly. } } } } I hate to be so down on this because I currently support an SEM at a } } high school because I firmly believe that having the students have the } } opportunity to play with the toys that we get to play with in the real } } world is a great way to inspire them to get the education they need. } } However, in addition to not seeing some major components to the system } } in your photos (you also need someone to evalute what's there), I can } } tell you that I once packed a system for a customer on a tight budget. } } I packed it for shipment by padded van (which is a much less intensive } } packing job than for a basic truck), but they decided to just put it on } } an Estes 18 wheeler despite what I had told them. By the time it had gone } from PA to CA it was trashed. } } Penny wise and pound foolish. And they had PAID for that system! } } } } Unfortunately aquiring a free SEM is an expensive affair unless you can } } find someone to do it all properly because THEY are also committed to } } the students. It's a sizeable commitment to take on even one SEM } } pro-bono, but maybe someone in your area could contact you, if they're } interested. } } Personally, I would like to see the manufacturers take on a high school } } system pro-bono for every x number of field service engineers, but that } } probably won't ever happen. (Are you guys paying attention, here?). } } } } I sincerely hope that someone DOES contact you and I also hope that my } } doom and gloom is off the mark. } } } } Best wishes } } Ken Converse } } Owner } } } } QUALITY IMAGES } } Servicing Scanning Electron Microscopes Since 1981 } } 474 So. Bridgton Rd. } } Bridgton, ME 04009 } } 207-647-4348 } } Fax 207-647-2688 } } kenconverse-at-qualityimages.biz } } qualityimages.biz } } } } } } } } -----Original Message----- } } X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com] } } Sent: Saturday, March 17, 2007 2:28 PM } } To: kenconverse-at-qualityimages.biz } } Subject: Re: [Microscopy] RE: New SEM donated to our lab- Can you } } identify the accessory? } } } } } } It's in New Jersey right now, and unfortunately we don't have the } } budget to send someone up there to pack it properly. The company who } } is donating it will not spend more than a few minutes prepping it (They } } aren't making money off of it, and they are electronics recyclers, so } } they just see it as a pile of metal) so I need to make instructions as } } simple and clear as possible, without having too much for them to do, } } or they may just decide to Ebay the whole thing. } } } } --Justin. } } } } On 3/17/07, kenconverse-at-qualityimages.biz } } {kenconverse-at-qualityimages.biz} } } wrote: } } } } } } } } } } } } } } } --------------------------------------------------------------------- } } } - } } } ------ } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } ----------------------------------------------------------------------- } } ----- } } } } } } Justin, } } } I believe what you have is a JXA-840 (microprobe) with 2 wavelength } } } spectrometers and a light microscope for establishing the proper WD } } } for using those spectrometers. The Box on top is an ion pump so that } } } the system can be used with a LaB6 cathode, if you choose (and have } } } received the correct wehnelt cap). } } } } } } Whether it is an 840 or 840A could be told by a look at the } } } electronics console, but you don't have any pictures of that or the } } } electronics rack that should be present for the spectometers. I do } } } see the power supply console in a couple of pictures. Does it } } } include the rotary pump and possibly compressor? } } } } } } As far as prepping for shipping, the first thing that jumps out at } } } me is that the table isn't bolted down! At a minimum you need to } } } get 4 M16x2.00x40mm bolts and 16mm washers to secure the table. You } } } also need to remove the magnet from the ion pump. Actually, most of } } } the column should be removed and packed separately unless you're } } } planning to move it only a few miles at very low speed. I also } } } suspect that the WDS units and light microscope should be removed. } } } All 3 are complex and fragile. } } } } } } Is it diffusion pumped or turbo pumped? If it's turbo pumped, the } } } pump must be removed before shipping. Also the high voltage oil tank } } } in the electronics console must be removed and the circuit board on } } } its side protected from damage. } } } } } } This is going to the W. Palm Beach area of Florida. Where is it } } } currently located? I would highly recommend sending someone to pack } } } it properly. How is it going to be shipped? If it's going by truck, } } } it must be "padded van" unless you're going to have a lot more of it } } } disassembled, crated and put on pallets. } } } } } } With more info, I might be of more help. } } } } } } Ken Converse } } } owner } } } } } } QUALITY IMAGES } } } Servicing Scanning Electron Microscopes Since 1981 } } } 474 So. Bridgton Rd. } } } Bridgton, ME 04009 } } } 207-647-4348 } } } Fax 207-647-2688 } } } kenconverse-at-qualityimages.biz } } } qualityimages.biz } } } } } } } } } } } } -----Original Message----- } } } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] } } } Sent: Friday, March 16, 2007 6:43 PM } } } To: kenconverse-at-qualityimages.biz } } } Subject: [Microscopy] New SEM donated to our lab- Can you identify the } } } accessory? } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------- } } } ------ } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } --------------------------------------------------------------------------- } - } } } } } } Hello all, } } } } } } We have a new SEM on the way to us, it's a JEOL JSM-840. All I have } } } at this point are a couple of pictures, and I am a little flummoxed } } } about the accessories that are added on this instrument. I recognize } } } the secondary electron detector, and I believe that there is a } } } backscatter detector on it, but I'm not sure what the other } } } accessories are. Also, the photos that I have seen of the basic 840s } } } don't include a box sitting on top of the column, but this one has it. } } } It's a silver-ish box with black sides, and is barely visible in one } } } of the photos, but it is there, at a slight angle. (Which leads me to } } } the next question- how should it be secured for } } } shipping?) } } } } } } The whole microscope is being packed by people who don't necessarily } } } know how to package it. Do any of you have specific suggestions of } } } what they could do to prevent damage in shipping? } } } } } } Here is the URL to the photos of the microscope (Hosted on my personal } } } page which has not been updated in a long time...) } } } http://www.jkraft.net/JEOL/ } } } } } } Thanks, } } } } } } Justin A. Kraft } } } Director } } } Center for Inquiry-Based Science Education } } } } } } ==============================Original } } } Headers============================== } } } 6, 26 -- From kraftpiano-at-gmail.com Fri Mar 16 17:40:22 2007 } } } 6, 26 -- Received: from ik-out-1112.google.com (ik-out-1112.google.com } } } [66.249.90.177]) } } } 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP } } id } } } l2GMeL9w006622 } } } 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 } 17:40:22 } } } -0500 } } } 6, 26 -- Received: by ik-out-1112.google.com with SMTP id b32so541347ika } } } 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 } } 15:40:20 } } } -0700 (PDT) } } } 6, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; } } } 6, 26 -- d=gmail.com; s=beta; } } } 6, 26 -- } } } } } h=domainkey-signature:received:received:message-id:date:from:to:subject:mim } e } } } -version:content-type:content-transfer-encoding:content-disposition; } } } 6, 26 -- } } } } } b=s1DejygYHazI0gOXcGdlH3aFhc31xI8o6mt+j5GOcTzrxtJPW9PLWzsfWcdgsZQjbUfGwio8N } U } } } weB8pHDxwgatI0zP5kv+TIfnL1G2A6U75mJ90Wu2/xog0gZFV7g5sBV3vJe5jiayVV4dGS } } } weB8pHDxwgatI0zP5kv+kp74tv } } } q2VQyDtZdd3Icvrv93WZs= } } } 6, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } } } 6, 26 -- d=gmail.com; s=beta; } } } 6, 26 -- } } } h=received:message-id:date:from:to:subject:mime-version:content-type:c } } } ontent } } } -transfer-encoding:content-disposition; } } } 6, 26 -- } } } } } b=pR3ppcp1LBgkdhNL2mxb8fxkll/KXvwAFHADQW/u6FINK4J15EsY55ex1+B2+g0JNvff5T3ga } a } } } YDWFX6PzBDM720L+slCXtxDVdzFMBAPEocfGL+xIvvPiSQg1ACUQ8+ujyW+Q/J0di/cxKy } } } YDWFX6PzBDM720L+slCXtxDVdzFMBAPEocfGL+xIvvPiSQg1ACUQ8+ujyW+HEXk7+ } } } jE105VLOBzCHBFkml2E+0= } } } 6, 26 -- Received: by 10.114.171.1 with SMTP id } } t1mr918673wae.1174084819773; } } } 6, 26 -- Fri, 16 Mar 2007 15:40:19 -0700 (PDT) } } } 6, 26 -- Received: by 10.114.79.12 with HTTP; Fri, 16 Mar 2007 } } } 15:40:19 -0700 (PDT) 6, 26 -- Message-ID: } } } {25e2b0d20703161540k140001d5k6170427b9d645d6b-at-mail.gmail.com} } } } 6, 26 -- Date: Fri, 16 Mar 2007 18:40:19 -0400 } } } 6, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} } } } 6, 26 -- To: microscopy-at-microscopy.com } } } 6, 26 -- Subject: New SEM donated to our lab- Can you identify the } } } accessory? 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: } } } text/plain; charset=ISO-8859-1; format=flowed 6, 26 -- } } } Content-Transfer-Encoding: 7bit 6, 26 -- Content-Disposition: inline } } } ==============================End of - } } } Headers============================== } } } } } } } } } } } } } } } } } } _________________________________________________________________ } } } Need personalized email and website? 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Learn more at www.doteasy.com } } } } } } } } } ==============================Original } } } Headers============================== } } } 28, 29 -- From kenconverse-at-qualityimages.biz Sat Mar 17 11:36:36 2007 } } } 28, 29 -- Received: from dpmailmta02.doteasy.com } (dpmailmta02.doteasy.com } } [65.61.209.90]) } } } 28, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP } } id l2HGaaU1026076 } } } 28, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sat, 17 Mar 2007 } } 11:36:36 -0500 } } } 28, 29 -- Received: from qualityimages.biz (unverified [192.168.101.16]) } } } 28, 29 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id } } 67056218-1814644 } } } 28, 29 -- for multiple; Sat, 17 Mar 2007 09:46:58 -0800 } } } 28, 29 -- Received: from Ken [76.179.237.214] by qualityimages.biz with } } ESMTP } } } 28, 29 -- (SMTPD32-8.05) id A90E1B650056; Sat, 17 Mar 2007 09:36:30 } } -0700 } } } 28, 29 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } } } 28, 29 -- To: {kraftpiano-at-gmail.com} , "MSA Listserver" } } {Microscopy-at-MSA.Microscopy.Com} } } } 28, 29 -- Subject: RE: [Microscopy] New SEM donated to our lab- Can you } } identify the accessory? } } } 28, 29 -- Date: Sat, 17 Mar 2007 12:36:18 -0400 } } } 28, 29 -- Message-ID: {004001c768b2$654b4e90$6401a8c0-at-Ken} } } } 28, 29 -- MIME-Version: 1.0 } } } 28, 29 -- Content-Type: text/plain; } } } 28, 29 -- charset="us-ascii" } } } 28, 29 -- X-Priority: 3 (Normal) } } } 28, 29 -- X-MSMail-Priority: Normal } } } 28, 29 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 } } } 28, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 } } } 28, 29 -- Thread-Index: AcdoHH2p3h+gQ0uxT2OvjUqyLu6bXwAkPKTg } } } 28, 29 -- Importance: Normal } } } 28, 29 -- In-Reply-To: {200703162243.l2GMhGZg011302-at-ns.microscopy.com} } } } 28, 29 -- X-IMSTrailer: __IMail_7__ } } } 28, 29 -- X-IP-stats: Incoming Last 0, First 170, in=3711794, out=0, } } spam=0 ip=192.168.101.16 } } } 28, 29 -- X-Originating-IP: 192.168.101.16 } } } 28, 29 -- Content-Transfer-Encoding: 8bit } } } 28, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id l2HGaaU1026076 } } } ==============================End of - } } Headers============================== } } } } } } } } } } } } } } } _________________________________________________________________ } } Need personalized email and website? 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Learn more at www.doteasy.com } } } } } } ==============================Original } Headers============================== } } 19, 30 -- From kenconverse-at-qualityimages.biz Tue Mar 20 10:06:44 2007 } } 19, 30 -- Received: from dpmailmta02.doteasy.com } } (dpmailmta02.doteasy.com [65.61.209.90]) } } 19, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id l2KF6ixi023551 } } 19, 30 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 20 Mar 2007 } } 10:06:44 -0500 } } 19, 30 -- Received: from qualityimages.biz (unverified [192.168.101.16]) } } 19, 30 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id } 67624751-1814644 } } 19, 30 -- for multiple; Tue, 20 Mar 2007 08:18:45 -0800 } } 19, 30 -- Received: from Ken [76.179.237.214] by qualityimages.biz with } ESMTP } } 19, 30 -- (SMTPD32-8.05) id A87ACAD00CA; Tue, 20 Mar 2007 08:06:34 -0700 } } 19, 30 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } } 19, 30 -- To: "'Justin Kraft'" {kraftpiano-at-gmail.com} , } } 19, 30 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} } } 19, 30 -- Subject: RE: [Microscopy] RE: New SEM donated to our lab- } } Can you identify the accessory? } } 19, 30 -- Date: Tue, 20 Mar 2007 11:06:24 -0400 } } 19, 30 -- Message-ID: {00ab01c76b01$56367fa0$6401a8c0-at-Ken} } } 19, 30 -- MIME-Version: 1.0 } } 19, 30 -- Content-Type: text/plain; } } 19, 30 -- charset="us-ascii" } } 19, 30 -- X-Priority: 3 (Normal) } } 19, 30 -- X-MSMail-Priority: Normal } } 19, 30 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 } } 19, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 } } 19, 30 -- Thread-Index: AcdowgMzeUfB1aHNRPeHEVz/wVFmXQCOd2qw } } 19, 30 -- Importance: Normal } } 19, 30 -- In-Reply-To: } } {25e2b0d20703171128t18b4ab53saff6cd38c5ed9343-at-mail.gmail.com} } } 19, 30 -- X-IMSTrailer: __IMail_7__ } } 19, 30 -- X-IP-stats: Incoming Last 0, First 173, in=3747649, out=0, } } spam=0 ip=192.168.101.16 } } 19, 30 -- X-Originating-IP: 192.168.101.16 } } 19, 30 -- Content-Transfer-Encoding: 8bit } } 19, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id l2KF6ixi023551 } } ==============================End of - } Headers============================== } } } -- } ############################################################## } John J. Bozzola, Ph.D., Director } I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) } 750 Communications Drive - MC 4402 } Southern Illinois University } Carbondale, IL 62901 U.S.A. } Phone: 618-453-3730 } Email: bozzola-at-siu.edu } ############################################################## } } ==============================Original Headers============================== } 8, 21 -- From bozzola-at-siu.edu Tue Mar 20 14:01:00 2007 } 8, 21 -- Received: from abbmta2.siu.edu (abbmta2.siu.edu [131.230.254.206]) } 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l2KJ0xQa012045 } 8, 21 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 20 Mar 2007 } 14:01:00 -0500 } 8, 21 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu } [131.230.177.142]) } 8, 21 -- by abbmta2.siu.edu (Switch-3.1.11/Switch-3.1.11) with ESMTP } id l2KJ0jnX013016 } 8, 21 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 20 Mar 2007 } 14:00:46 -0500 (CDT) } 8, 21 -- Mime-Version: 1.0 } 8, 21 -- X-Sender: bozzola-at-saluki-mail.siu.edu } 8, 21 -- Message-Id: {p0611040bc225ddd7c662-at-[131.230.177.142]} } 8, 21 -- In-Reply-To: {200703201507.l2KF7sPB025219-at-ns.microscopy.com} } 8, 21 -- References: {200703201507.l2KF7sPB025219-at-ns.microscopy.com} } 8, 21 -- Date: Tue, 20 Mar 2007 14:00:44 -0500 } 8, 21 -- To: Microscopy-at-msa.microscopy.com } 8, 21 -- From: "John J. Bozzola" {bozzola-at-siu.edu} } 8, 21 -- Subject: Re: [Microscopy] New SEM donated to our lab- Can you } identify } 8, 21 -- the accessory? } 8, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } 8, 21 -- X-Spam-Score: 0.00% } 8, 21 -- X-MASF: 0.00% } 8, 21 -- X-Whitelist: 0.00% } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 19, 22 -- From walck-at-southbaytech.com Tue Mar 20 15:22:30 2007 } 19, 22 -- Received: from flpi102.sbcis.sbc.com (flpi102.sbcis.sbc.com [207.115.20.71]) } 19, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2KKMTSh004709 } 19, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Mar 2007 15:22:30 -0500 } 19, 22 -- X-ORBL: [64.169.217.123] } 19, 22 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) } 19, 22 -- by flpi102.sbcis.sbc.com (8.13.8 out.dk.spool/8.13.8) with ESMTP id l2KKMIAn018852; } 19, 22 -- Tue, 20 Mar 2007 13:22:19 -0700 } 19, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} } 19, 22 -- To: {bozzola-at-siu.edu} } 19, 22 -- Cc: {Microscopy-at-microscopy.com} } 19, 22 -- Subject: RE: [Microscopy] Re: New SEM donated to our lab- Moving a microscope -For Fun } 19, 22 -- Date: Tue, 20 Mar 2007 13:21:06 -0800 } 19, 22 -- Message-ID: {004d01c76b35$acc02220$7801a8c0-at-dynamicbl8uno3} } 19, 22 -- MIME-Version: 1.0 } 19, 22 -- Content-Type: text/plain; } 19, 22 -- charset="us-ascii" } 19, 22 -- Content-Transfer-Encoding: 7bit } 19, 22 -- X-Mailer: Microsoft Office Outlook 11 } 19, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 } 19, 22 -- In-Reply-To: {200703201905.l2KJ5dII017968-at-ns.microscopy.com} } 19, 22 -- Thread-Index: AcdrIsAkh+8+WaVTTsebGWA8my5x1gAEl67A } ==============================End of - Headers============================== }
==============================Original Headers============================== 10, 29 -- From kraftpiano-at-gmail.com Tue Mar 20 15:55:28 2007 10, 29 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.230]) 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2KKtS5r016903 10, 29 -- for {microscopy-at-microscopy.com} ; Tue, 20 Mar 2007 15:55:28 -0500 10, 29 -- Received: by wx-out-0506.google.com with SMTP id s16so17982wxc 10, 29 -- for {microscopy-at-microscopy.com} ; Tue, 20 Mar 2007 13:55:27 -0700 (PDT) 10, 29 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 10, 29 -- d=gmail.com; s=beta; 10, 29 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:cc:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 10, 29 -- b=IQ5wDgCQ1YPA+u4I9ySK6Iu5yAYgBW4t+75LsOgsg7ckwLcwa8jSujlH5hS3Oc+5ATS2R4iPPzBIVEA01SvEHBedBe0en3+Avo+KCcuF4BtlfsSe6/LUrUgID3/9dIciLavjYJ+ht677OjBKMGay0wsN+0vBVIfbUNvKVF+iiRY= 10, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 10, 29 -- d=gmail.com; s=beta; 10, 29 -- h=received:message-id:date:from:to:subject:cc:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 10, 29 -- b=R+CDtBfLmIOzYm5aMV7Yn7JOc8EQ0yo5GGvQDrxe1gRGJPzFinVju//g5D4QBNSojjaVRKFBsJKqBpsCRW/SRruHtGrX5K8uDWlbq9HRoh0zSko5fnfy+y0e0G54R8qBylmbAd6oyYKgke8caiSJb4Un2+4O+3nExT5or3RgVqI= 10, 29 -- Received: by 10.90.49.19 with SMTP id w19mr1504890agw.1174424125211; 10, 29 -- Tue, 20 Mar 2007 13:55:25 -0700 (PDT) 10, 29 -- Received: by 10.114.79.12 with HTTP; Tue, 20 Mar 2007 13:55:24 -0700 (PDT) 10, 29 -- Message-ID: {25e2b0d20703201355n4725b776x4767b0895039b518-at-mail.gmail.com} 10, 29 -- Date: Tue, 20 Mar 2007 16:55:24 -0400 10, 29 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 10, 29 -- To: walck-at-southbaytech.com 10, 29 -- Subject: Re: [Microscopy] New SEM donated to our lab- Moving a microscope -For Fun 10, 29 -- Cc: microscopy-at-microscopy.com 10, 29 -- In-Reply-To: {200703202030.l2KKUHVK016106-at-ns.microscopy.com} 10, 29 -- MIME-Version: 1.0 10, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 29 -- Content-Transfer-Encoding: 7bit 10, 29 -- Content-Disposition: inline 10, 29 -- References: {200703202030.l2KKUHVK016106-at-ns.microscopy.com} ==============================End of - Headers==============================
If the column has a turbo, they need to tape it in the center position using duct tape. One basically make an X arrangement using lots of tape around the pump and side steel columns. This has worked for me in the past several moves. If they don't do this, you can kiss the bellows goodbye.
If you can't get a good mech pump, let me know. I have several lying around here that are not being used. I have a Varian and some other brand that are the same or greater capacity as Edwards RV8. Worst case is shipping cost and potential need to rebuild the pump vanes. Pump is free. You can get rebuild kits from Dunniway Vacuum.
gary g.
At 12:57 PM 3/20/2007, you wrote:
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==============================Original Headers============================== 11, 21 -- From gary-at-gaugler.com Tue Mar 20 16:22:14 2007 11, 21 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2KLMECw028777 11, 21 -- for {microscopy-at-microscopy.com} ; Tue, 20 Mar 2007 16:22:14 -0500 11, 21 -- Message-Id: {200703202122.l2KLMECw028777-at-ns.microscopy.com} 11, 21 -- Received: (qmail 31374 invoked from network); 20 Mar 2007 14:22:12 -0700 11, 21 -- Received: by simscan 1.1.0 ppid: 31371, pid: 31372, t: 0.1312s 11, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 21 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 21 -- by qsmtp2 with SMTP; 20 Mar 2007 14:22:12 -0700 11, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 21 -- Date: Tue, 20 Mar 2007 14:22:10 -0800 11, 21 -- To: kraftpiano-at-gmail.com 11, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 21 -- Subject: Re: [Microscopy] Re: New SEM donated to our lab- Moving a 11, 21 -- microscope -For Fun 11, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 21 -- In-Reply-To: {200703202057.l2KKvMPq019820-at-ns.microscopy.com} 11, 21 -- References: {200703202057.l2KKvMPq019820-at-ns.microscopy.com} 11, 21 -- Mime-Version: 1.0 11, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-5933D79 ==============================End of - Headers==============================
Justin, I'm feeling better since you seem to have an idea of what you're up against. Enough doom and gloom - I'm keeping my fingers crossed for you.
As to the pump: JEOL automatically vents the RP line upon shut-down and normally also shuts off the RP. Their RPs (rotary pumps, same as MP - mechanical pump) run on 200VAC. A 240VAC motor may complain terminally. One alternative I have used is to build a relay box that uses their 200VAC control signal but allows you to use a 120VAC Welch, Edwards, Alcatel (pick your flavor) pump and not end up with the system shutting down and your pump running continuously on a major leak. I can send you some info on that. I suppose a buck/boost transformer might also do the trick.
It sound like you aren't getting the JEOL toolkit, which is too bad. If you send me your snailmail address I will send you schematics. I will also throw in a CD with both the user's manual and the service manual. This will open on MS Word document reader. If you need Adobe Acrobat, let me know but it will take a little longer, but I can do that for you.
We can make school fun!
Ken converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Tuesday, March 20, 2007 4:58 PM To: kenconverse-at-qualityimages.biz
I was very amused by that quip as well. Of course, I was also amused that they kept calling it an "Electron Scanner Microscope."
Oh, well.
Thanks for all of the replies, guys. Unfortunately, I have little to no control over the packing. They will take basic suggestions, and do some basic things, but generally they are in a different line of business- electronics recycling. They were put in the position of having it and not knowing how to sell it, so they ended up giving it to me. The way I figure, I'll have them batten it down as best as they are willing to do, and hope. (Logically, since according to them it was taken out some 50 miles or so away from where they are, and they took it out, I figure that any damage that will be done to it has already been done.)
They're splitting the shipping costs with us as well, so the total out of pocket expense for us is $300.
Worst case scenario I end up with a bunch of broken parts, but the basis for putting together a working instrument over the next several years. My degree is in physics, and so I will at least enjoy the challenge of figuring it out from a circuits and wires point of view.
They don't have any manuals or anything- all I'm getting is the column, the display console, and the power supply box. They sold the pump box (Probably because that was something they recognized) on Ebay, so my first challenge will be getting a pump up and running. I might steal the one off of the ISI SX-40A I have at home for a little while. After that, there is an air conditioning service vacuum sales representative who is willing to donate one of his pumps and some gauges and such.
When the SEM gets here, I'll post regular updates (If you're interested) on our progress. One of my fortes is documentation, so I will at least document the whole process on a website.
Doom and gloom predictions aside now, let's all hold our breaths and cross our collective fingers and toes.
--Justin.
On 3/20/07, walck-at-southbaytech.com {walck-at-southbaytech.com} wrote: } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } You all have to hire the guys that moved the microscope on the TV } show, "Crossing Jordan". } } In the story line, they had moved the EM out of the lab for cost } cutting and had it in the basement. When they needed it, the boss } told them to "go get my microscope" and they had it up and running in } less than an hour. } } Well, I thought it was funny! } } } -Scott } } Scott D. Walck, Ph.D. } Technical Director } South Bay Technology, Inc. } 1120 Via Callejon } San Clemente, CA 92673 } } US Toll Free: 1-800-728-2233 } Tel: (949) 492-2600 } Fax: (949) 492-1499 } } www.southbaytech.com } walck-at-southbaytech.com } } -----Original Message----- } X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] } Sent: Tuesday, March 20, 2007 11:06 AM } To: Walck-at-SouthBayTech.com } Subject: [Microscopy] Re: New SEM donated to our lab- Can you identify } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Ken, } } You make a VERY IMPORTANT point here: "Getting the instrument is the } easy part. Getting it up and running consistently well takes a lot of } work." } } In my SEM course, I always take time to point out to my students that } they may be in a situation where someone wants to donate an EM to } their school. No matter how well it is working when the donation is } accepted, there is no guarantee that it will ever work properly in the } new location unless professionals are involved in the packing, } shipping and installation. I always recommend having $5-10K available. } Hopefully, it will never be needed. My analogy is: "It's like having a } baby. The easy part is producing the baby. Caring for it is a labor } (hopefully of love)." } } JB } } } } Justin, } } Unless they are paying for the shipping, you're going to be out a } } sizeable chunk of change just in the shipping costs. If someone } } doesn't spend at least a few hours properly packing the system for } } however it is going to be shipped (and how it will be shipped makes a } } difference in how it is packed), you will receive a large anchor for } } your boat. Unless you have someone at your end who is experienced } } and willing to donate their time for getting this system back up and } } running, you probably don't have the budget to get it running if you } } don't } have the budget to have it packed properly. } } } } I hate to be so down on this because I currently support an SEM at a } } high school because I firmly believe that having the students have } } the opportunity to play with the toys that we get to play with in the } } real world is a great way to inspire them to get the education they } } need. However, in addition to not seeing some major components to the } } system in your photos (you also need someone to evalute what's } } there), I can tell you that I once packed a system for a customer on } } a tight budget. I packed it for shipment by padded van (which is a } } much less intensive packing job than for a basic truck), but they } } decided to just put it on an Estes 18 wheeler despite what I had told } } them. By the time it had gone } from PA to CA it was trashed. } } Penny wise and pound foolish. And they had PAID for that system! } } } } Unfortunately aquiring a free SEM is an expensive affair unless you } } can find someone to do it all properly because THEY are also } } committed to the students. It's a sizeable commitment to take on } } even one SEM pro-bono, but maybe someone in your area could contact } } you, if they're } interested. } } Personally, I would like to see the manufacturers take on a high } } school system pro-bono for every x number of field service engineers, } } but that probably won't ever happen. (Are you guys paying attention, } } here?). } } } } I sincerely hope that someone DOES contact you and I also hope that } } my doom and gloom is off the mark. } } } } Best wishes } } Ken Converse } } Owner } } } } QUALITY IMAGES } } Servicing Scanning Electron Microscopes Since 1981 } } 474 So. Bridgton Rd. } } Bridgton, ME 04009 } } 207-647-4348 } } Fax 207-647-2688 } } kenconverse-at-qualityimages.biz } } qualityimages.biz } } } } } } } } -----Original Message----- } } X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com] } } Sent: Saturday, March 17, 2007 2:28 PM } } To: kenconverse-at-qualityimages.biz } } Subject: Re: [Microscopy] RE: New SEM donated to our lab- Can you } } identify the accessory? } } } } } } It's in New Jersey right now, and unfortunately we don't have the } } budget to send someone up there to pack it properly. The company who } } is donating it will not spend more than a few minutes prepping it } } (They aren't making money off of it, and they are electronics } } recyclers, so they just see it as a pile of metal) so I need to make } } instructions as simple and clear as possible, without having too much } } for them to do, or they may just decide to Ebay the whole thing. } } } } --Justin. } } } } On 3/17/07, kenconverse-at-qualityimages.biz } } {kenconverse-at-qualityimages.biz} } } wrote: } } } } } } } } } } } } } } } ------------------------------------------------------------------- } } } -- } } } - } } } ------ } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help
==============================Original Headers============================== 10, 29 -- From kraftpiano-at-gmail.com Tue Mar 20 15:55:28 2007 10, 29 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.230]) 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2KKtS5r016903 10, 29 -- for {microscopy-at-microscopy.com} ; Tue, 20 Mar 2007 15:55:28 -0500 10, 29 -- Received: by wx-out-0506.google.com with SMTP id s16so17982wxc 10, 29 -- for {microscopy-at-microscopy.com} ; Tue, 20 Mar 2007 13:55:27 -0700 (PDT) 10, 29 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 10, 29 -- d=gmail.com; s=beta; 10, 29 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:cc:i n-reply-to:mime-version:content-type:content-transfer-encoding:content-dispo sition:references; 10, 29 -- b=IQ5wDgCQ1YPA+u4I9ySK6Iu5yAYgBW4t+75LsOgsg7ckwLcwa8jSujlH5hS3Oc+5ATS2R4iPPz BIVEA01SvEHBedBe0en3+Avo+KCcuF4BtlfsSe6/LUrUgID3/9dIciLavjYJ+ht677OjBKMGay0w sN+0vBVIfbUNvKVF+iiRY= 10, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 10, 29 -- d=gmail.com; s=beta; 10, 29 -- h=received:message-id:date:from:to:subject:cc:in-reply-to:mime-version:conte nt-type:content-transfer-encoding:content-disposition:references; 10, 29 -- b=R+CDtBfLmIOzYm5aMV7Yn7JOc8EQ0yo5GGvQDrxe1gRGJPzFinVju//g5D4QBNSojjaVRKFBsJ KqBpsCRW/SRruHtGrX5K8uDWlbq9HRoh0zSko5fnfy+y0e0G54R8qBylmbAd6oyYKgke8caiSJb4 Un2+4O+3nExT5or3RgVqI= 10, 29 -- Received: by 10.90.49.19 with SMTP id w19mr1504890agw.1174424125211; 10, 29 -- Tue, 20 Mar 2007 13:55:25 -0700 (PDT) 10, 29 -- Received: by 10.114.79.12 with HTTP; Tue, 20 Mar 2007 13:55:24 -0700 (PDT) 10, 29 -- Message-ID: {25e2b0d20703201355n4725b776x4767b0895039b518-at-mail.gmail.com} 10, 29 -- Date: Tue, 20 Mar 2007 16:55:24 -0400 10, 29 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 10, 29 -- To: walck-at-southbaytech.com 10, 29 -- Subject: Re: [Microscopy] New SEM donated to our lab- Moving a microscope -For Fun 10, 29 -- Cc: microscopy-at-microscopy.com 10, 29 -- In-Reply-To: {200703202030.l2KKUHVK016106-at-ns.microscopy.com} 10, 29 -- MIME-Version: 1.0 10, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 29 -- Content-Transfer-Encoding: 7bit 10, 29 -- Content-Disposition: inline 10, 29 -- References: {200703202030.l2KKUHVK016106-at-ns.microscopy.com} ==============================End of - Headers==============================
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==============================Original Headers============================== 30, 29 -- From kenconverse-at-qualityimages.biz Tue Mar 20 17:05:58 2007 30, 29 -- Received: from dpmailmta02.doteasy.com (dpmailmta02.doteasy.com [65.61.209.90]) 30, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2KM5wtA008952 30, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 20 Mar 2007 17:05:58 -0500 30, 29 -- Received: from qualityimages.biz (unverified [192.168.101.16]) 30, 29 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id 67727501-1814644 30, 29 -- for multiple; Tue, 20 Mar 2007 15:18:07 -0800 30, 29 -- Received: from Ken [76.179.237.214] by qualityimages.biz with ESMTP 30, 29 -- (SMTPD32-8.05) id AABDF5D0114; Tue, 20 Mar 2007 15:05:49 -0700 30, 29 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 30, 29 -- To: {kraftpiano-at-gmail.com} , "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 30, 29 -- Subject: RE: [Microscopy] Re: New SEM donated to our lab- Moving a microscope -For Fun 30, 29 -- Date: Tue, 20 Mar 2007 18:05:37 -0400 30, 29 -- Message-ID: {00b901c76b3b$e73fd070$6401a8c0-at-Ken} 30, 29 -- MIME-Version: 1.0 30, 29 -- Content-Type: text/plain; 30, 29 -- charset="us-ascii" 30, 29 -- X-Priority: 3 (Normal) 30, 29 -- X-MSMail-Priority: Normal 30, 29 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 30, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 30, 29 -- Thread-Index: AcdrMoGWLVrUh0vfSwytQS42e6qJQQABrMKA 30, 29 -- Importance: Normal 30, 29 -- In-Reply-To: {200703202058.l2KKwNeM021611-at-ns.microscopy.com} 30, 29 -- X-IMSTrailer: __IMail_7__ 30, 29 -- X-IP-stats: Incoming Last 0, First 174, in=3753621, out=0, spam=0 ip=192.168.101.16 30, 29 -- X-Originating-IP: 192.168.101.16 30, 29 -- Content-Transfer-Encoding: 8bit 30, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2KM5wtA008952 ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (paul-at-biochem.bumc.bu.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, March 20, 2007 at 16:59:28 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both paul-at-biochem.bumc.bu.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: paul-at-biochem.bumc.bu.edu Name: Paul Toselli
Organization: Boston University Medical School
Education: Graduate College
Location: Boston, MA
Title: Plastic Embedment of Mouse Lens & Eye for TEM
Question: I would like a method to fix and Araldite/DDSA-embed a mouse eye for examination under a transmission electron microscope. The mouse would be approximately one-month of age. Would you please help me? Thank you.
The new Auger Spectrometer is a newly designed one and we just want to test it to get some obvious Auger peaks, like the carbon or oxide. And the availability of Auger Spectrometer in a SEM vacuum is one purpose of this design as well. I know it is hard to work in that vacuum pressure, but we do provide some methods to clean the sample in situ.
Cheers
Charles
} From: colijn.1-at-osu.edu } Reply-To: colijn.1-at-osu.edu } To: charlesping-at-hotmail.com } Subject: [Microscopy] Re: JEOL 6400F SEM need help for the interface } Date: Tue, 20 Mar 2007 08:56:02 -0500 } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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==============================Original Headers============================== 8, 23 -- From charlesping-at-hotmail.com Wed Mar 21 05:53:56 2007 8, 23 -- Received: from bay0-omc3-s3.bay0.hotmail.com (bay0-omc3-s3.bay0.hotmail.com [65.54.246.203]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2LArtwi016061 8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Mar 2007 05:53:55 -0500 8, 23 -- Received: from hotmail.com ([64.4.51.28]) by bay0-omc3-s3.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2668); 8, 23 -- Wed, 21 Mar 2007 03:53:55 -0700 8, 23 -- Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; 8, 23 -- Wed, 21 Mar 2007 03:53:54 -0700 8, 23 -- Message-ID: {BAY107-F18C0805443F9B13EF9145FBD740-at-phx.gbl} 8, 23 -- Received: from 64.4.51.220 by by107fd.bay107.hotmail.msn.com with HTTP; 8, 23 -- Wed, 21 Mar 2007 10:53:54 GMT 8, 23 -- X-Originating-IP: [144.32.136.9] 8, 23 -- X-Originating-Email: [charlesping-at-hotmail.com] 8, 23 -- X-Sender: charlesping-at-hotmail.com 8, 23 -- In-Reply-To: {200703201356.l2KDu22l021630-at-ns.microscopy.com} 8, 23 -- From: "xiaoping zha" {charlesping-at-hotmail.com} 8, 23 -- To: colijn.1-at-osu.edu 8, 23 -- Cc: Microscopy-at-microscopy.com 8, 23 -- Subject: RE: [Microscopy] Re: JEOL 6400F SEM need help for the interface 8, 23 -- Date: Wed, 21 Mar 2007 10:53:54 +0000 8, 23 -- Mime-Version: 1.0 8, 23 -- Content-Type: text/plain; format=flowed 8, 23 -- X-OriginalArrivalTime: 21 Mar 2007 10:53:54.0857 (UTC) FILETIME=[381E5190:01C76BA7] ==============================End of - Headers==============================
Could folks with HKL EBSD systems with Hitachi SEMs please contact me directly (johnf-at-geology.wisc.edu). I am particularly interested in which version of the KE bse amplifier they were supplied with. Apparently there are different KE versions built for different sems. We are continuing to try to determine the source of 200 KHZ noise on bse images coming from that amplifier.
John -- ======================================================== John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480 Cameron Electron Microprobe Lab lab: (608) 265-4798 Dept of Geology & Geophysics fax: (608) 262-0693 University of Wisconsin home: (608) 274-2245 1215 West Dayton St. email: johnf-at-geology.wisc.edu Madison, WI 53706 amateur radio: WA3BTA Personal http://www.geology.wisc.edu/~johnf/ Probe lab http://www.geology.wisc.edu/~johnf/sx51.html Probe Sign Up Calender: http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi
"The first rule of all intelligent tinkering is to save every cog and wheel." -- Aldo Leopold
"For a successful technology, reality must take precedence over public relations, for Nature cannot be fooled." -- Richard P. Feynman
==============================Original Headers============================== 4, 23 -- From johnf-at-geology.wisc.edu Wed Mar 21 10:27:55 2007 4, 23 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2LFRrJb001687 4, 23 -- for {microscopy-at-microscopy.com} ; Wed, 21 Mar 2007 10:27:54 -0500 4, 23 -- Received: from localhost (localhost [127.0.0.1]) 4, 23 -- by localhost (Postfix) with ESMTP id 3DF8820D14; 4, 23 -- Wed, 21 Mar 2007 10:27:51 -0500 (CDT) 4, 23 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) 4, 23 -- by localhost (geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 4, 23 -- with ESMTP id 18433-05; Wed, 21 Mar 2007 10:27:39 -0500 (CDT) 4, 23 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57]) 4, 23 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 4, 23 -- (No client certificate requested) 4, 23 -- by ice.geology.wisc.edu (Postfix) with ESMTP id D25F320D0A; 4, 23 -- Wed, 21 Mar 2007 10:27:39 -0500 (CDT) 4, 23 -- Mime-Version: 1.0 4, 23 -- Message-Id: {p06230905c226fea56628-at-[144.92.206.57]} 4, 23 -- Date: Wed, 21 Mar 2007 10:27:36 -0500 4, 23 -- To: microscopy-at-microscopy.com 4, 23 -- From: John Fournelle {johnf-at-geology.wisc.edu} 4, 23 -- Subject: HKL EBSD with Hitachi SEMs 4, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 23 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu ==============================End of - Headers==============================
Ok. The company has contacted me and told me that I need to pay more money to have the microscope packaged, otherwise they will just strap it to the pallets and ship it out. Is there anybody in or around Brockport, NY who wouldn't mind going over and taking the ion pump off the top of the instrument? They refuse to do it unless I pay extra. ("I can only guess that it will take three to four hours for someone to do that")
The ion pump is hanging at a strange angle, and I'm worried that it will do major damage if left on.
Help!
--Justin A. Kraft Director Center for Inquiry-Based Science Education
==============================Original Headers============================== 4, 26 -- From kraftpiano-at-gmail.com Wed Mar 21 14:01:09 2007 4, 26 -- Received: from ug-out-1314.google.com (ug-out-1314.google.com [66.249.92.168]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2LJ18Mp019744 4, 26 -- for {microscopy-at-microscopy.com} ; Wed, 21 Mar 2007 14:01:09 -0500 4, 26 -- Received: by ug-out-1314.google.com with SMTP id m2so428608ugc 4, 26 -- for {microscopy-at-microscopy.com} ; Wed, 21 Mar 2007 12:01:08 -0700 (PDT) 4, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 4, 26 -- d=gmail.com; s=beta; 4, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 26 -- b=ftitQdfAMJ4pt6QMBCUIM4/iA4PzO9yyHk5aH+sUlINaledh67SNPkmEi2i+Gk3Tq9BpoT74ULtSZQFIANhNmoEyqXfcpKE28ldtKj0pnol3JohzR3eWKQCSYpjjd77s6uyZepXSgXt1fTxNU6AP3ntj51xqeZmgCTGMQ4BAJho= 4, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 26 -- d=gmail.com; s=beta; 4, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 26 -- b=OZwOWLhn0RnvytbSjHq29I78e90Y1Yar2az+LInInm3VFYDTsUUDemIsZrZ7zBljOpiSUBzie87uENuEG1QhZjNWJhC9ftvlspDPWhmaA1GcHiep01Vzn6bNzhpigPFtnNvrnmjn3Ef9PWFr8rUq/27epOo2/QMd/orgGwr7zIk= 4, 26 -- Received: by 10.114.211.1 with SMTP id j1mr277166wag.1174503667454; 4, 26 -- Wed, 21 Mar 2007 12:01:07 -0700 (PDT) 4, 26 -- Received: by 10.114.79.12 with HTTP; Wed, 21 Mar 2007 12:01:07 -0700 (PDT) 4, 26 -- Message-ID: {25e2b0d20703211201t4324eba3h9d3942a1b7895a96-at-mail.gmail.com} 4, 26 -- Date: Wed, 21 Mar 2007 15:01:07 -0400 4, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 4, 26 -- To: microscopy-at-microscopy.com 4, 26 -- Subject: AAArgh: SEM Donation gone horribly awry! Cany anyone help?!?!? 4, 26 -- MIME-Version: 1.0 4, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 26 -- Content-Transfer-Encoding: 7bit 4, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
I just got off of a lengthy phone conversation with the gentleman at the warehouse who is shipping out our new SEM. He was insisting that he needed more money to remove the magnets from the ion pump, since he didn't even know what it was.
He walked out to the instrument in his warehouse, and I explained it to him, at which point he removed the magnets personally since THERE WERE NO SCREWS HOLDING THEM IN!
After which he replied "Was that it?"
I thanked him, and our microscope will be beginning its journey on Friday.
Everyone can breathe a sigh of relief for a moment, but keep the fingers crossed about the WDS crystals...
--Justin A. Kraft
==============================Original Headers============================== 6, 26 -- From kraftpiano-at-gmail.com Wed Mar 21 16:18:30 2007 6, 26 -- Received: from ug-out-1314.google.com (ug-out-1314.google.com [66.249.92.175]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2LLIQlk002549 6, 26 -- for {microscopy-at-microscopy.com} ; Wed, 21 Mar 2007 16:18:29 -0500 6, 26 -- Received: by ug-out-1314.google.com with SMTP id m2so471074ugc 6, 26 -- for {microscopy-at-microscopy.com} ; Wed, 21 Mar 2007 14:18:25 -0700 (PDT) 6, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 6, 26 -- d=gmail.com; s=beta; 6, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 6, 26 -- b=uuMppxFeL01ge14cBjYmaHzNiFmVxRaVij0l9PcgMYGcxkbMaaoobbcFR1WnxM+QCqV0VzAuTvaxl4NztJCsX2jEyHknhIg3cQPOPlha5TUJxJXkGDkVzzVaF9oHx9YkwbDr68ZyeGI57L2yGP5wKXLPComi09STgVbplPMjrWE= 6, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 26 -- d=gmail.com; s=beta; 6, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 6, 26 -- b=kGCXRThhbK3v0uupLMdRem75WWuO96ewCg4QV0RkFupapwI+9FG57HAGEtnpLswHyUEj8cop83ajAUbhDuZahrJnq2hz2HRMTlPGbWuuAXPTdDgD1z/yi3mWzdlADsAI4JoB3fF2VqWo+FRzMADCTPW4X17FTKOs4UGnuCJ/QbU= 6, 26 -- Received: by 10.114.124.1 with SMTP id w1mr320973wac.1174511900237; 6, 26 -- Wed, 21 Mar 2007 14:18:20 -0700 (PDT) 6, 26 -- Received: by 10.114.79.12 with HTTP; Wed, 21 Mar 2007 14:18:20 -0700 (PDT) 6, 26 -- Message-ID: {25e2b0d20703211418s315d8b27r6d73590fcab296f7-at-mail.gmail.com} 6, 26 -- Date: Wed, 21 Mar 2007 17:18:20 -0400 6, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 6, 26 -- To: microscopy-at-microscopy.com 6, 26 -- Subject: Update: Microscope saved by savvy straight-talk. 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 26 -- Content-Transfer-Encoding: 7bit 6, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both zhang.zaoli-at-google.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: Ulm university, electron microscopy center
Title-Subject: [Filtered] From PEELS in Tia to EELS in Digital Micrograph
Question: Dear Colleagues
I acquired EELS spectra in STEM mode using TIA software, I have no idea on how to transfer EELS spectra in TIA(STEM) to Digital Micrograph for further processing, Does anyone know how to do that ?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rosenj03-at-med.nyu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rosenj03-at-med.nyu.edu Name: J. Rosenbluth
Organization: NYU School of Med.
Title-Subject: [Filtered] TEM
Question: Looking for someone to service Sorval MT2 and MT5000 microtomes in New York City (Manhattan).
FEI should supply that information or insist that you want to acquire data with digitalMicrosgraph!!
Customer demands move company hands and heads!
Roseann Csencsits
On Mar 21, 2007, at 3:49 PM, zhang.zaoli-at-google.com wrote:
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==============================Original Headers============================== 6, 26 -- From RCsencsits-at-lbl.gov Wed Mar 21 18:09:20 2007 6, 26 -- Received: from ironport1.lbl.gov (ironport1.lbl.gov [128.3.41.47]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2LN9KVn006315 6, 26 -- for {microscopy-at-microscopy.com} ; Wed, 21 Mar 2007 18:09:20 -0500 6, 26 -- Received: from mta1.lbl.gov ([128.3.41.24]) 6, 26 -- by ironport1.lbl.gov with ESMTP; 21 Mar 2007 16:09:19 -0700 6, 26 -- X-Ironport-SBRS: None 6, 26 -- X-BrightmailFiltered: true 6, 26 -- X-Brightmail-Tracker: AAAAAA== 6, 26 -- X-IronPort-AV: i="4.14,310,1170662400"; 6, 26 -- d="scan'208"; a="28560902:sNHT22838767" 6, 26 -- Received: from [131.243.35.34] (0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.34]) 6, 26 -- by mta1.lbl.gov (8.13.8/8.13.8) with ESMTP id l2LN9I6F021446; 6, 26 -- Wed, 21 Mar 2007 16:09:19 -0700 (PDT) 6, 26 -- In-Reply-To: {200703212249.l2LMnYO1002647-at-ns.microscopy.com} 6, 26 -- References: {200703212249.l2LMnYO1002647-at-ns.microscopy.com} 6, 26 -- Mime-Version: 1.0 (Apple Message framework v752.3) 6, 26 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 26 -- Message-Id: {B0614BC6-333E-4780-B70B-F6ACFF2BC2FE-at-lbl.gov} 6, 26 -- Cc: microscopy-at-microscopy.com 6, 26 -- Content-Transfer-Encoding: 7bit 6, 26 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov} 6, 26 -- Subject: Re: [Microscopy] [Filtered] MicroscopyListserverviaWWW: From PEELS in Tia to 6, 26 -- Date: Wed, 21 Mar 2007 16:07:28 -0700 6, 26 -- To: zhang.zaoli-at-google.com 6, 26 -- X-Mailer: Apple Mail (2.752.3) ==============================End of - Headers==============================
Question: Looking for someone to service Sorval MT2 and MT5000 microtomes in New York City (Manhattan).
We have been very satisfied with:
MOC (Microscopical Optical Consulting, Inc) P.O. Box 586 Valley Cottage, NY 10989
MOCLeica.aol.com
Phone: either 845-268-6450 or 914-268-6450 (I have two cards, and I think the 845 area code is the more recent
Excellent service, good pricing
Don
-- _____________________________________________________________ Donald L. Lovett E-mail: Lovett-at-tcnj.edu Professor Phone: 609-771-2876 Department of Biology Fax: 609-637-5118 The College of New Jersey P.O. Box 7718 Ewing, NJ 08628-0718
==============================Original Headers============================== 10, 25 -- From lovett-at-tcnj.edu Thu Mar 22 07:34:34 2007 10, 25 -- Received: from cyrus.TCNJ.EDU (cyrus.TCNJ.EDU [159.91.15.208]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2MCYMp1002853 10, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Mar 2007 07:34:33 -0500 10, 25 -- Received: from mppd (localhost [127.0.0.1]) 10, 25 -- by localhost.scannedby.clamav (Postfix) with SMTP id 78DCDC219; 10, 25 -- Thu, 22 Mar 2007 08:34:14 -0400 (EDT) 10, 25 -- Received: from [159.91.98.99] (TCNJ-98-99.TCNJ.EDU [159.91.98.99]) 10, 25 -- by cyrus.TCNJ.EDU (Postfix) with ESMTP id 466FDC20D; 10, 25 -- Thu, 22 Mar 2007 08:34:14 -0400 (EDT) 10, 25 -- Message-ID: {460277B7.5070504-at-tcnj.edu} 10, 25 -- Date: Thu, 22 Mar 2007 08:33:59 -0400 10, 25 -- From: "Donald L. Lovett" {lovett-at-tcnj.edu} 10, 25 -- Organization: Department of Biology, The College of New Jersey 10, 25 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 10, 25 -- X-Accept-Language: en-us, en 10, 25 -- MIME-Version: 1.0 10, 25 -- To: rosenj03-at-med.nyu.edu, Microscopy-at-microscopy.com 10, 25 -- Subject: Re: [Microscopy] viaWWW: Sorval MT2 and MT5000 microtomes 10, 25 -- References: {200703212244.l2LMiCrh019005-at-ns.microscopy.com} 10, 25 -- In-Reply-To: {200703212244.l2LMiCrh019005-at-ns.microscopy.com} 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 25 -- Content-Transfer-Encoding: 7bit 10, 25 -- X-Scanned-By: RAE MPP/Clamd http://www.messagepartners.com 10, 25 -- X-Scanned-By: This message was scanned by MPP v.2 (www.messagepartners.com) ==============================End of - Headers==============================
Do you really want to fix and embed the whole eye? Even assuming you could cut wrinkle-free thin sections of such a large block I don't think the section would fit on a grid. AND, with such a large section, you would never find what you were looking for in the EM. I suggest defining your research target more precisely. Also consider colaborating with an anatomist/morphologist skilled in EM. If you want to look at the lens you should know that the lens is very difficult to infiltrate so Araddite/DDSA may not be the best choice of epoxy. Go the to library and get a good textbook of ophthamology (or even your old histology text from medical school). The references will tell you what those who have already attacked this problem have done as far as fixation, embedding, etc. Why re-invent the wheel?
Geoff
paul-at-biochem.bumc.bu.edu wrote:
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-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 9, 36 -- From mcauliff-at-umdnj.edu Thu Mar 22 09:08:10 2007 9, 36 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 9, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2ME8AFL016262 9, 36 -- for {microscopy-at-msa.microscopy.com} ; Thu, 22 Mar 2007 09:08:10 -0500 9, 36 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 9, 36 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 7CC991C336C 9, 36 -- for {microscopy-at-msa.microscopy.com} ; Thu, 22 Mar 2007 10:08:09 -0400 (EDT) 9, 36 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) 9, 36 -- by zix01.umdnj.edu (Proprietary) with ESMTP id 283D1A7B6E 9, 36 -- for {microscopy-at-msa.microscopy.com} ; Thu, 22 Mar 2007 10:08:08 -0400 (EDT) 9, 36 -- Received: from ([130.219.34.131]) 9, 36 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.88226783; 9, 36 -- Thu, 22 Mar 2007 10:07:48 -0400 9, 36 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 9, 36 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 9, 36 -- id {0JFB00K015RGUV-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 9, 36 -- for microscopy-at-msa.microscopy.com; Thu, 22 Mar 2007 10:07:48 -0400 (EDT) 9, 36 -- Received: from [127.0.0.1] ([10.138.2.123]) 9, 36 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 9, 36 -- 2004)) with ESMTP id {0JFB00JD85X0UL-at-Polaris.umdnj.edu} ; Thu, 9, 36 -- 22 Mar 2007 10:07:48 -0400 (EDT) 9, 36 -- Date: Thu, 22 Mar 2007 10:09:20 -0400 9, 36 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 9, 36 -- Subject: Re: [Microscopy] AskAMicroscopist: Plastic Embedment of Mouse Lens & 9, 36 -- Eye for TEM 9, 36 -- In-reply-to: {200703202354.l2KNsHYb024638-at-ns.microscopy.com} 9, 36 -- To: paul-at-biochem.bumc.bu.edu, 9, 36 -- MicroscopyListserver {microscopy-at-msa.microscopy.com} 9, 36 -- Message-id: {46028E10.7010703-at-umdnj.edu} 9, 36 -- MIME-version: 1.0 9, 36 -- Content-type: text/plain; format=flowed; charset=us-ascii 9, 36 -- Content-transfer-encoding: 7BIT 9, 36 -- X-Accept-Language: en-us, en 9, 36 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 9, 36 -- Gecko/20040804 Netscape/7.2 (ax) 9, 36 -- References: {200703202354.l2KNsHYb024638-at-ns.microscopy.com} ==============================End of - Headers==============================
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Email: rosenj03-at-med.nyu.edu Name: J. Rosenbluth
Organization: NYU School of Med.
Title-Subject: [Filtered] TEM
Question: Looking for someone to service Sorval MT2 and MT5000 microtomes in New York City (Manhattan).
==============================Original Headers============================== 6, 11 -- From zaluzec-at-microscopy.com Wed Mar 21 17:43:34 2007 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2LMhXxP016804 6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 21 Mar 2007 17:43:33 -0500 6, 11 -- Mime-Version: 1.0 6, 11 -- Message-Id: {p06240801c2276581a9ec-at-[206.69.208.22]} 6, 11 -- Date: Wed, 21 Mar 2007 17:43:32 -0500 6, 11 -- To: microscopy-at-microscopy.com 6, 11 -- From: rosenj03-at-med.nyu.edu (by way of MicroscopyListserver) 6, 11 -- Subject: viaWWW: Sorval MT2 and MT5000 microtomes 6, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
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==============================Original Headers============================== 33, 29 -- From MSHERWOOD-at-PARTNERS.ORG Thu Mar 22 09:15:10 2007 33, 29 -- Received: from phsmgmx2.partners.org (phsmgmx2.partners.org [155.52.251.33]) 33, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2MEF9eI027390 33, 29 -- for {microscopy-at-msa.microscopy.com} ; Thu, 22 Mar 2007 09:15:09 -0500 33, 29 -- Received: from phsmgmx2.partners.org (localhost.localdomain [127.0.0.1]) 33, 29 -- by localhost (Postfix) with SMTP id 7109D3E01C5 33, 29 -- for {microscopy-at-msa.microscopy.com} ; Thu, 22 Mar 2007 10:15:09 -0400 (EDT) 33, 29 -- Received: from PHSXCON5.partners.org (phsxcon5.mgh.harvard.edu [132.183.130.38]) 33, 29 -- by phsmgmx2.partners.org (Postfix) with ESMTP id 4570A3E01BF 33, 29 -- for {microscopy-at-msa.microscopy.com} ; Thu, 22 Mar 2007 10:15:08 -0400 (EDT) 33, 29 -- Received: from PHSXMB1.partners.org ([132.183.118.117]) by PHSXCON5.partners.org with Microsoft SMTPSVC(6.0.3790.1830); 33, 29 -- Thu, 22 Mar 2007 10:15:07 -0400 33, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 33, 29 -- Content-class: urn:content-classes:message 33, 29 -- MIME-Version: 1.0 33, 29 -- Content-Type: text/plain; 33, 29 -- charset="iso-8859-1" 33, 29 -- Subject: FW: [Microscopy] viaWWW: Sorval MT2 and MT5000 microtomes 33, 29 -- Date: Thu, 22 Mar 2007 10:15:07 -0400 33, 29 -- Message-ID: {1AF23D0AD12E7444A5DB083CA978B73407A31041-at-PHSXMB1.partners.org} 33, 29 -- X-MS-Has-Attach: 33, 29 -- X-MS-TNEF-Correlator: 33, 29 -- Thread-Topic: [Microscopy] viaWWW: Sorval MT2 and MT5000 microtomes 33, 29 -- Thread-Index: AcdsCt6iJQDynJSMTG6dTjc3OBl86gAgF0XAAAA8bmA= 33, 29 -- From: "Sherwood, Margaret " {MSHERWOOD-at-PARTNERS.ORG} 33, 29 -- To: {microscopy-at-msa.microscopy.com} 33, 29 -- X-OriginalArrivalTime: 22 Mar 2007 14:15:07.0857 (UTC) FILETIME=[7E99AC10:01C76C8C] 33, 29 -- Content-Transfer-Encoding: 8bit 33, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2MEF9eI027390 ==============================End of - Headers==============================
Whilst this is good advice from Geoff, fixing and processing the whole eye has its advantages in minimising disruption to the internal structures, and in particular the retinal layers.
Whole eye however, is extremely difficult to infiltrate and the great variation in structure density makes this a challenging proposition.
The following procedure improved our LM sectioning results, and I would suggest giving it a try for EM processing.
Following initial fixation at 4'C in 2.5% glut, trim a superficial slice off one side of the eye - just enough to expose the inner cavity. We use indented bluetack to hold the eye in position whilst slicing with a razor blade in a line parallel to the plane of the optic nerve which is pointing down into the bluetack.
Don't be tempted to go for a second parallel slice as this causes a lot of disruption to the internal structures, and return the eye to fix for a further overnight period at 4'C. Follow this with a prolonged processing schedule using a low viscosity epoxy resin.
If (safe) Spurr is no longer available you could try TAAB's low viscosity epoxy resin (www.taab.co.uk). We have already tested this product as an alternative to Spurr for when our supplies run out. It is slightly more viscous than Spurr but readily available.
I would then suggest doing a final dissection to remove the lens (if not required) and to obtain blocks that are of a reasonable size for ultrathin sectioning and to include your area of interest. If this includes the retinal layer, it would probably be useful to include a bit of the optic nerve as an identifier. I would then suggest returning the samples to fresh resin for a final infiltration with pure resin (under vacuum at 50'C) for an hour or so, prior to transferring to embedding moulds and polymerising at 60'C for 24-48hrs. Flat embedding will probably work best if it is a cross section you are going for.
Contact me direct if you want to follow our prolonged epoxy resin schedule.
Hope this helps.
Alastair
Alastair McKinnon Histology & EM Facility Manager University of Aberdeen, Institute of Medical Science Foresterhill, Aberdeen, AB25 2ZD tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk www.abdn.ac.uk/ims/h-em -----Original Message----- X-from: mcauliff-at-umdnj.edu [mailto:mcauliff-at-umdnj.edu] Sent: 22 March 2007 14:14 To: Mckinnon, Alastair D.
Greetings!
Do you really want to fix and embed the whole eye? Even assuming you could cut wrinkle-free thin sections of such a large block I don't think the section would fit on a grid. AND, with such a large section, you would never find what you were looking for in the EM. I suggest defining your research target more precisely. Also consider colaborating with an anatomist/morphologist skilled in EM. If you want to look at the lens you should know that the lens is very difficult to infiltrate so Araddite/DDSA may not be the best choice of epoxy. Go the to library and get a good textbook of ophthamology (or even your old histology text from medical school). The references will tell you what those who have already attacked this problem have done as far as fixation, embedding, etc. Why re-invent the wheel?
Geoff
paul-at-biochem.bumc.bu.edu wrote:
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-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 9, 36 -- From mcauliff-at-umdnj.edu Thu Mar 22 09:08:10 2007 9, 36 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 9, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2ME8AFL016262 9, 36 -- for {microscopy-at-msa.microscopy.com} ; Thu, 22 Mar 2007 09:08:10 -0500 9, 36 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 9, 36 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 7CC991C336C 9, 36 -- for {microscopy-at-msa.microscopy.com} ; Thu, 22 Mar 2007 10:08:09 -0400 (EDT) 9, 36 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) 9, 36 -- by zix01.umdnj.edu (Proprietary) with ESMTP id 283D1A7B6E 9, 36 -- for {microscopy-at-msa.microscopy.com} ; Thu, 22 Mar 2007 10:08:08 -0400 (EDT) 9, 36 -- Received: from ([130.219.34.131]) 9, 36 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.88226783; 9, 36 -- Thu, 22 Mar 2007 10:07:48 -0400 9, 36 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 9, 36 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 9, 36 -- id {0JFB00K015RGUV-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 9, 36 -- for microscopy-at-msa.microscopy.com; Thu, 22 Mar 2007 10:07:48 -0400 (EDT) 9, 36 -- Received: from [127.0.0.1] ([10.138.2.123]) 9, 36 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 9, 36 -- 2004)) with ESMTP id {0JFB00JD85X0UL-at-Polaris.umdnj.edu} ; Thu, 9, 36 -- 22 Mar 2007 10:07:48 -0400 (EDT) 9, 36 -- Date: Thu, 22 Mar 2007 10:09:20 -0400 9, 36 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 9, 36 -- Subject: Re: [Microscopy] AskAMicroscopist: Plastic Embedment of Mouse Lens & 9, 36 -- Eye for TEM 9, 36 -- In-reply-to: {200703202354.l2KNsHYb024638-at-ns.microscopy.com} 9, 36 -- To: paul-at-biochem.bumc.bu.edu, 9, 36 -- MicroscopyListserver {microscopy-at-msa.microscopy.com} 9, 36 -- Message-id: {46028E10.7010703-at-umdnj.edu} 9, 36 -- MIME-version: 1.0 9, 36 -- Content-type: text/plain; format=flowed; charset=us-ascii 9, 36 -- Content-transfer-encoding: 7BIT 9, 36 -- X-Accept-Language: en-us, en 9, 36 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 9, 36 -- Gecko/20040804 Netscape/7.2 (ax) 9, 36 -- References: {200703202354.l2KNsHYb024638-at-ns.microscopy.com} ==============================End of - Headers==============================
==============================Original Headers============================== 27, 24 -- From a.d.mckinnon-at-abdn.ac.uk Thu Mar 22 10:41:18 2007 27, 24 -- Received: from mailhub2.abdn.ac.uk (mailhub2.abdn.ac.uk [139.133.7.24]) 27, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2MFfHbq015040 27, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Mar 2007 10:41:17 -0500 27, 24 -- Received: from ew-mail-a.uoa.abdn.ac.uk ([139.133.15.20] helo=VMAIL1.uoa.abdn.ac.uk) 27, 24 -- by mailhub2.abdn.ac.uk with esmtp (Exim 4.52) 27, 24 -- id 1HUPPf-0004L3-W7; Thu, 22 Mar 2007 15:41:16 +0000 27, 24 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 27, 24 -- Content-class: urn:content-classes:message 27, 24 -- MIME-Version: 1.0 27, 24 -- Content-Type: text/plain; 27, 24 -- charset="us-ascii" 27, 24 -- Subject: RE: [Microscopy] Re: AskAMicroscopist: Plastic Embedment of Mouse Lens & 27, 24 -- Date: Thu, 22 Mar 2007 15:38:56 -0000 27, 24 -- Message-ID: {7CA99BD491B8EF45BBF158CAEF74653388575A-at-VMAIL1.uoa.abdn.ac.uk} 27, 24 -- X-MS-Has-Attach: 27, 24 -- X-MS-TNEF-Correlator: 27, 24 -- Thread-Topic: [Microscopy] Re: AskAMicroscopist: Plastic Embedment of Mouse Lens & 27, 24 -- Thread-Index: AcdsjEcPO/9epX8kRmKe7fjgpWlpfwABQ5jA 27, 24 -- From: "Mckinnon, Alastair D." {a.d.mckinnon-at-abdn.ac.uk} 27, 24 -- To: {mcauliff-at-umdnj.edu} 27, 24 -- Cc: {Microscopy-at-microscopy.com} 27, 24 -- Content-Transfer-Encoding: 8bit 27, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2MFfHbq015040 ==============================End of - Headers==============================
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Email: rosenj03-at-med.nyu.edu Name: J. Rosenbluth
Organization: NYU School of Medicine
Title-Subject: [Filtered] TEM
Question: Would appreciate suggestions re organizations competent to service: 1. JEOL CX100 2. Philips EM300
Does any one know of service for the same microtomes and a LKB Bromma 8300 in the Los Angeles area? Thanks,
Ani } } } ------------------------------------------------------------------ ---------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 3, 29 -- From ani.issaian-at-csun.edu Thu Mar 22 17:46:53 2007 3, 29 -- Received: from plover.csun.edu (plover.csun.edu [130.166.1.24]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2MMkrPV028825 3, 29 -- for {microscopy-at-microscopy.com} ; Thu, 22 Mar 2007 17:46:53 -0500 3, 29 -- Received: from puffin.csun.edu (puffin.csun.edu [130.166.1.21]) 3, 29 -- by plover.csun.edu (MOS 3.8.2-GA) 3, 29 -- with ESMTP id DQB56450; 3, 29 -- Thu, 22 Mar 2007 15:46:52 -0700 (PDT) 3, 29 -- Received: from cuckoo.csun.edu (cuckoo.csun.edu [130.166.1.230]) 3, 29 -- by puffin.csun.edu (MOS 3.7.5a-GA) 3, 29 -- with ESMTP id FOI26107; 3, 29 -- Thu, 22 Mar 2007 15:46:51 -0700 (PDT) 3, 29 -- Received: (from cuckoo.csun.edu [130.166.114.86]) 3, 29 -- by cuckoo.csun.edu (MOS 3.8.2-GA) 3, 29 -- with HTTPS/1.1 id AWS58669 (AUTH ami24015); 3, 29 -- Thu, 22 Mar 2007 15:46:50 -0700 (PDT) 3, 29 -- From: Ani M Issaian {ani.issaian-at-csun.edu} 3, 29 -- Subject: Re: [Microscopy] viaWWW: Sorval MT2 and MT5000 3, 29 -- microtomes 3, 29 -- To: rosenj03-at-med.nyu.edu 3, 29 -- Cc: microscopy-at-microscopy.com 3, 29 -- Reply-To: ani.issaian-at-csun.edu 3, 29 -- X-Mailer: Mirapoint Webmail Direct 3.8.2-GA 3, 29 -- MIME-Version: 1.0 3, 29 -- Content-Type: text/plain; charset=us-ascii 3, 29 -- Content-Transfer-Encoding: 7bit 3, 29 -- Message-Id: {20070322154650.AWS58669-at-cuckoo.csun.edu} 3, 29 -- Date: Thu, 22 Mar 2007 15:46:50 -0700 (PDT) 3, 29 -- X-Junkmail-Whitelist: YES (by domain whitelist at plover.csun.edu) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Tanya.Hayes-at-northampton.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Tanya.Hayes-at-northampton.ac.uk Name: Tanya Hayes
Organization: The University of Northampton
Title-Subject: [Filtered] SEM: lines on slow scan/capture
Question: We have an Hitachi S3000N SEM. We have intermittent lines appearing across our images, mainly sourcing in bands from brighter areas, all the way across the image. This has got much worse over the last few weeks. We have ruled out electrical interference & vibrations. Has anyone got any ideas?
Tanya Hayes British School of Leather Technology The University of Northampton Northampton United Kingdom
if you have ruled out simple charging effects then maybe you have a similar fault to us.
We have a similar intermittent problem on our S3000N which is associated with fluctuation in the filament emission readout. It doesn't seem to be specific to either high pressure or high vacuum mode but sometimes will stabilise if run at a higher voltage and dropped back down - makes it seem like minor contamination. Generally this "flickering" effect gets progressively worse with the age of the filament (but is it an effect of age or is the flickering affecting the life of the filament?).
The trouble is the effect could be self-fulfilling because the shortened filament life increases gun contamination and so may help to induce the effect.
The erratic nature of the problem has made it difficult to solve or indeed know if it has really gone away. I had hoped that it might either go away or deteriorate to a point where the cause could be found.
Sorry - no real answers and I can't be sure from your description if we even have a similar fault. But I will be interested in any progress you make or suggestions from the readership.
Oh one thing that seems to make the problem worse (although may not be the only cause) is that the top of the Wenhelt cap can slowly work a little loose and although it may not be the only cause just gently nipping it tight can help.
Good luck and if there's anything else that you need help with I'm sure I can be equally as informative (or not). Please contact me directly by email if you want a phone number.
Malcolm
Malcolm Haswell e.m. unit Chemispec School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: Tanya.Hayes-at-northampton.ac.uk
Just in case the enclosed link is unknown to the listserver, I am sending it along on this thread. I think anyone who is attempting to reassemble an old EM should get a smile and perhaps inspiration from the sincere efforts of this fellow: http://www.home.neab.net/gandalf/EM-lab/TEM100CX/
I would also be interested in learning if anyone has found similar diary's on the web that could be shared with the listserver.
Cheers
Roger A. Ristau, PhD Electron Microscopy Specialist Institute of Materials Science 97 North Eagleville Road University of Connecticut Storrs, CT 06269 vox: 860-486-5379 fax: 860-486-4745
} From: kraftpiano-at-gmail.com } Reply-To: kraftpiano-at-gmail.com } Date: Tue, 20 Mar 2007 16:00:13 -0500 } To: raristau-at-ims.uconn.edu } Subject: [Microscopy] Re: New SEM donated to our lab- Moving a microscope -For } Fun } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I was very amused by that quip as well. Of course, I was also amused } that they kept calling it an "Electron Scanner Microscope." } } Oh, well. } } Thanks for all of the replies, guys. Unfortunately, I have little to } no control over the packing. They will take basic suggestions, and do } some basic things, but generally they are in a different line of } business- electronics recycling. They were put in the position of } having it and not knowing how to sell it, so they ended up giving it } to me. The way I figure, I'll have them batten it down as best as } they are willing to do, and hope. (Logically, since according to them } it was taken out some 50 miles or so away from where they are, and } they took it out, I figure that any damage that will be done to it has } already been done.) } } They're splitting the shipping costs with us as well, so the total out } of pocket expense for us is $300. } } Worst case scenario I end up with a bunch of broken parts, but the } basis for putting together a working instrument over the next several } years. My degree is in physics, and so I will at least enjoy the } challenge of figuring it out from a circuits and wires point of view. } } They don't have any manuals or anything- all I'm getting is the } column, the display console, and the power supply box. They sold the } pump box (Probably because that was something they recognized) on } Ebay, so my first challenge will be getting a pump up and running. I } might steal the one off of the ISI SX-40A I have at home for a little } while. After that, there is an air conditioning service vacuum sales } representative who is willing to donate one of his pumps and some } gauges and such. } } When the SEM gets here, I'll post regular updates (If you're } interested) on our progress. One of my fortes is documentation, so I } will at least document the whole process on a website. } } Doom and gloom predictions aside now, let's all hold our breaths and } cross our collective fingers and toes. } } --Justin. } } On 3/20/07, walck-at-southbaytech.com {walck-at-southbaytech.com} wrote: } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } You all have to hire the guys that moved the microscope on the TV show, } } "Crossing Jordan". } } } } In the story line, they had moved the EM out of the lab for cost cutting and } } had it in the basement. When they needed it, the boss told them to "go get } } my microscope" and they had it up and running in less than an hour. } } } } Well, I thought it was funny! } } } } } } -Scott } } } } Scott D. Walck, Ph.D. } } Technical Director } } South Bay Technology, Inc. } } 1120 Via Callejon } } San Clemente, CA 92673 } } } } US Toll Free: 1-800-728-2233 } } Tel: (949) 492-2600 } } Fax: (949) 492-1499 } } } } www.southbaytech.com } } walck-at-southbaytech.com } } } } -----Original Message----- } } X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] } } Sent: Tuesday, March 20, 2007 11:06 AM } } To: Walck-at-SouthBayTech.com } } Subject: [Microscopy] Re: New SEM donated to our lab- Can you identify } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Ken, } } } } You make a VERY IMPORTANT point here: "Getting the instrument is the easy } } part. Getting it up and running consistently well takes a lot of work." } } } } In my SEM course, I always take time to point out to my students that they } } may be in a situation where someone wants to donate an EM to their school. } } No matter how well it is working when the donation is accepted, there is no } } guarantee that it will ever work properly in the new location unless } } professionals are involved in the packing, shipping and installation. I } } always recommend having $5-10K available. Hopefully, it will never be } } needed. My analogy is: "It's like having a baby. The easy part is producing } } the baby. Caring for it is a labor (hopefully of love)." } } } } JB } } } } } } } Justin, } } } Unless they are paying for the shipping, you're going to be out a } } } sizeable chunk of change just in the shipping costs. If someone } } } doesn't spend at least a few hours properly packing the system for } } } however it is going to be shipped (and how it will be shipped makes a } } } difference in how it is packed), you will receive a large anchor for } } } your boat. Unless you have someone at your end who is experienced and } } } willing to donate their time for getting this system back up and } } } running, you probably don't have the budget to get it running if you don't } } have the budget to have it packed properly. } } } } } } I hate to be so down on this because I currently support an SEM at a } } } high school because I firmly believe that having the students have the } } } opportunity to play with the toys that we get to play with in the real } } } world is a great way to inspire them to get the education they need. } } } However, in addition to not seeing some major components to the system } } } in your photos (you also need someone to evalute what's there), I can } } } tell you that I once packed a system for a customer on a tight budget. } } } I packed it for shipment by padded van (which is a much less intensive } } } packing job than for a basic truck), but they decided to just put it on } } } an Estes 18 wheeler despite what I had told them. By the time it had gone } } from PA to CA it was trashed. } } } Penny wise and pound foolish. And they had PAID for that system! } } } } } } Unfortunately aquiring a free SEM is an expensive affair unless you can } } } find someone to do it all properly because THEY are also committed to } } } the students. It's a sizeable commitment to take on even one SEM } } } pro-bono, but maybe someone in your area could contact you, if they're } } interested. } } } Personally, I would like to see the manufacturers take on a high school } } } system pro-bono for every x number of field service engineers, but that } } } probably won't ever happen. (Are you guys paying attention, here?). } } } } } } I sincerely hope that someone DOES contact you and I also hope that my } } } doom and gloom is off the mark. } } } } } } Best wishes } } } Ken Converse } } } Owner } } } } } } QUALITY IMAGES } } } Servicing Scanning Electron Microscopes Since 1981 } } } 474 So. Bridgton Rd. } } } Bridgton, ME 04009 } } } 207-647-4348 } } } Fax 207-647-2688 } } } kenconverse-at-qualityimages.biz } } } qualityimages.biz } } } } } } } } } } } } -----Original Message----- } } } X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com] } } } Sent: Saturday, March 17, 2007 2:28 PM } } } To: kenconverse-at-qualityimages.biz } } } Subject: Re: [Microscopy] RE: New SEM donated to our lab- Can you } } } identify the accessory? } } } } } } } } } It's in New Jersey right now, and unfortunately we don't have the } } } budget to send someone up there to pack it properly. The company who } } } is donating it will not spend more than a few minutes prepping it (They } } } aren't making money off of it, and they are electronics recyclers, so } } } they just see it as a pile of metal) so I need to make instructions as } } } simple and clear as possible, without having too much for them to do, } } } or they may just decide to Ebay the whole thing. } } } } } } --Justin. } } } } } } On 3/17/07, kenconverse-at-qualityimages.biz } } } {kenconverse-at-qualityimages.biz} } } } wrote: } } } } } } } } } } } } } } } } } } } } --------------------------------------------------------------------- } } } } - } } } } ------ } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } } America } } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } } ----------------------------------------------------------------------- } } } ----- } } } } } } } } Justin, } } } } I believe what you have is a JXA-840 (microprobe) with 2 wavelength } } } } spectrometers and a light microscope for establishing the proper WD } } } } for using those spectrometers. The Box on top is an ion pump so that } } } } the system can be used with a LaB6 cathode, if you choose (and have } } } } received the correct wehnelt cap). } } } } } } } } Whether it is an 840 or 840A could be told by a look at the } } } } electronics console, but you don't have any pictures of that or the } } } } electronics rack that should be present for the spectometers. I do } } } } see the power supply console in a couple of pictures. Does it } } } } include the rotary pump and possibly compressor? } } } } } } } } As far as prepping for shipping, the first thing that jumps out at } } } } me is that the table isn't bolted down! At a minimum you need to } } } } get 4 M16x2.00x40mm bolts and 16mm washers to secure the table. You } } } } also need to remove the magnet from the ion pump. Actually, most of } } } } the column should be removed and packed separately unless you're } } } } planning to move it only a few miles at very low speed. I also } } } } suspect that the WDS units and light microscope should be removed. } } } } All 3 are complex and fragile. } } } } } } } } Is it diffusion pumped or turbo pumped? If it's turbo pumped, the } } } } pump must be removed before shipping. Also the high voltage oil tank } } } } in the electronics console must be removed and the circuit board on } } } } its side protected from damage. } } } } } } } } This is going to the W. Palm Beach area of Florida. Where is it } } } } currently located? I would highly recommend sending someone to pack } } } } it properly. How is it going to be shipped? If it's going by truck, } } } } it must be "padded van" unless you're going to have a lot more of it } } } } disassembled, crated and put on pallets. } } } } } } } } With more info, I might be of more help. } } } } } } } } Ken Converse } } } } owner } } } } } } } } QUALITY IMAGES } } } } Servicing Scanning Electron Microscopes Since 1981 } } } } 474 So. Bridgton Rd. } } } } Bridgton, ME 04009 } } } } 207-647-4348 } } } } Fax 207-647-2688 } } } } kenconverse-at-qualityimages.biz } } } } qualityimages.biz } } } } } } } } } } } } } } } } -----Original Message----- } } } } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] } } } } Sent: Friday, March 16, 2007 6:43 PM } } } } To: kenconverse-at-qualityimages.biz } } } } Subject: [Microscopy] New SEM donated to our lab- Can you identify the } } } } accessory? } } } } } } } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------- } } } } ------ } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } } --------------------------------------------------------------------------- } } - } } } } } } } } Hello all, } } } } } } } } We have a new SEM on the way to us, it's a JEOL JSM-840. All I have } } } } at this point are a couple of pictures, and I am a little flummoxed } } } } about the accessories that are added on this instrument. I recognize } } } } the secondary electron detector, and I believe that there is a } } } } backscatter detector on it, but I'm not sure what the other } } } } accessories are. Also, the photos that I have seen of the basic 840s } } } } don't include a box sitting on top of the column, but this one has it. } } } } It's a silver-ish box with black sides, and is barely visible in one } } } } of the photos, but it is there, at a slight angle. (Which leads me to } } } } the next question- how should it be secured for } } } } shipping?) } } } } } } } } The whole microscope is being packed by people who don't necessarily } } } } know how to package it. Do any of you have specific suggestions of } } } } what they could do to prevent damage in shipping? } } } } } } } } Here is the URL to the photos of the microscope (Hosted on my personal } } } } page which has not been updated in a long time...) } } } } http://www.jkraft.net/JEOL/ } } } } } } } } Thanks, } } } } } } } } Justin A. Kraft } } } } Director } } } } Center for Inquiry-Based Science Education } } } } } } } } ==============================Original } } } } Headers============================== } } } } 6, 26 -- From kraftpiano-at-gmail.com Fri Mar 16 17:40:22 2007 } } } } 6, 26 -- Received: from ik-out-1112.google.com (ik-out-1112.google.com } } } } [66.249.90.177]) } } } } 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP } } } id } } } } l2GMeL9w006622 } } } } 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 } } 17:40:22 } } } } -0500 } } } } 6, 26 -- Received: by ik-out-1112.google.com with SMTP id b32so541347ika } } } } 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Mar 2007 } } } 15:40:20 } } } } -0700 (PDT) } } } } 6, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; } } } } 6, 26 -- d=gmail.com; s=beta; } } } } 6, 26 -- } } } } } } } h=domainkey-signature:received:received:message-id:date:from:to:subject:mim } } e } } } } -version:content-type:content-transfer-encoding:content-disposition; } } } } 6, 26 -- } } } } } } } b=s1DejygYHazI0gOXcGdlH3aFhc31xI8o6mt+j5GOcTzrxtJPW9PLWzsfWcdgsZQjbUfGwio8N } } U } } } } weB8pHDxwgatI0zP5kv+TIfnL1G2A6U75mJ90Wu2/xog0gZFV7g5sBV3vJe5jiayVV4dGS } } } } weB8pHDxwgatI0zP5kv+kp74tv } } } } q2VQyDtZdd3Icvrv93WZs= } } } } 6, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } } } } 6, 26 -- d=gmail.com; s=beta; } } } } 6, 26 -- } } } } h=received:message-id:date:from:to:subject:mime-version:content-type:c } } } } ontent } } } } -transfer-encoding:content-disposition; } } } } 6, 26 -- } } } } } } } b=pR3ppcp1LBgkdhNL2mxb8fxkll/KXvwAFHADQW/u6FINK4J15EsY55ex1+B2+g0JNvff5T3ga } } a } } } } YDWFX6PzBDM720L+slCXtxDVdzFMBAPEocfGL+xIvvPiSQg1ACUQ8+ujyW+Q/J0di/cxKy } } } } YDWFX6PzBDM720L+slCXtxDVdzFMBAPEocfGL+xIvvPiSQg1ACUQ8+ujyW+HEXk7+ } } } } jE105VLOBzCHBFkml2E+0= } } } } 6, 26 -- Received: by 10.114.171.1 with SMTP id } } } t1mr918673wae.1174084819773; } } } } 6, 26 -- Fri, 16 Mar 2007 15:40:19 -0700 (PDT) } } } } 6, 26 -- Received: by 10.114.79.12 with HTTP; Fri, 16 Mar 2007 } } } } 15:40:19 -0700 (PDT) 6, 26 -- Message-ID: } } } } {25e2b0d20703161540k140001d5k6170427b9d645d6b-at-mail.gmail.com} } } } } 6, 26 -- Date: Fri, 16 Mar 2007 18:40:19 -0400 } } } } 6, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} } } } } 6, 26 -- To: microscopy-at-microscopy.com } } } } 6, 26 -- Subject: New SEM donated to our lab- Can you identify the } } } } accessory? 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: } } } } text/plain; charset=ISO-8859-1; format=flowed 6, 26 -- } } } } Content-Transfer-Encoding: 7bit 6, 26 -- Content-Disposition: inline } } } } ==============================End of - } } } } Headers============================== } } } } } } } } } } } } } } } } } } } } } } } } _________________________________________________________________ } } } } Need personalized email and website? Look no further. It's easy with } } } } Doteasy $0 Web Hosting! 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==============================Original Headers============================== 7, 17 -- From raristau-at-ims.uconn.edu Fri Mar 23 08:57:06 2007 7, 17 -- Received: from mail1.uits.uconn.edu (mail1.uits.uconn.edu [137.99.25.203]) 7, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2NDv61w016014 7, 17 -- for {microscopy-at-microscopy.com} ; Fri, 23 Mar 2007 08:57:06 -0500 7, 17 -- Received: from [137.99.20.118] (d20h118.public.uconn.edu [137.99.20.118]) 7, 17 -- by mail1.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id l2NDv5wG007149 7, 17 -- for {microscopy-at-microscopy.com} ; Fri, 23 Mar 2007 09:57:05 -0400 7, 17 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 7, 17 -- Date: Fri, 23 Mar 2007 08:57:03 -0500 7, 17 -- Subject: Re: New SEM donated to our lab- Moving a microscope -For Fun 7, 17 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 7, 17 -- To: {microscopy-at-microscopy.com} 7, 17 -- Message-ID: {C22946DF.2012%raristau-at-ims.uconn.edu} 7, 17 -- In-Reply-To: {200703202100.l2KL0DAH024896-at-ns.microscopy.com} 7, 17 -- Mime-version: 1.0 7, 17 -- Content-type: text/plain; charset="US-ASCII" 7, 17 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
Since you say it starts over bright objects, and is worse at slower scan rates it sounds like charging to me.
A couple of suggestions. Have you changed to a different type of specimen lately? Go back to an object you have successfully imaged before, or one that should have no chance of charging issues - like a calibration grating, or stick a copper TEM grid to a stub with silver or graphite paint, even just the aluminum stub surface (rough up with sandpaper to give a coarse texture), and see if it is still present. If gone, it was charging and it could be something in the specimen type (round objects or powders have contact/grounding issues and will be worse) or something in the prep - maybe something in the sputtering? Does the sample look reasonable - like it actually got sputtered? Note that samples will look different for the same sputtered layer if smooth vs. textured, white, etc.
If it is still present with samples that absolutely should not charge, then it is in the instrument; is the stage grounding good?
I had bands all the way across, not just from prominent bright details, and it was the spring contacts holding the filament - weak/oxidized - causing fluctuations. If you can rule out the sample, it could be any number of instrument related problems. But the "sourcing in bands from brighter areas" - if I understand your meaning -sounds like charging.
Dale
Tanya.Hayes-at-northampton.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both Tanya.Hayes-at-northampton.ac.uk as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: Tanya.Hayes-at-northampton.ac.uk } Name: Tanya Hayes } } Organization: The University of Northampton } } Title-Subject: [Filtered] SEM: lines on slow scan/capture } } Question: We have an Hitachi S3000N SEM. We have intermittent lines appearing across our images, mainly sourcing in bands from brighter areas, all the way across the image. This has got much worse over the last few weeks. We have ruled out electrical interference & vibrations. Has anyone got any ideas? } } Tanya Hayes } British School of Leather Technology } The University of Northampton } Northampton } United Kingdom } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Fri Mar 23 07:43:15 2007 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2NChETj024263 } 7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 23 Mar 2007 07:43:15 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240800c2297bd0e70c-at-[206.69.208.22]} } 7, 11 -- Date: Fri, 23 Mar 2007 07:43:13 -0500 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: Tanya.Hayes-at-northampton.ac.uk (by way of MicroscopyListserver) } 7, 11 -- Subject: viaWWW: SEM: lines on slow scan/capture } 7, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 22 -- From dac-at-research.umass.edu Fri Mar 23 09:34:25 2007 9, 22 -- Received: from race6.oit.umass.edu (race6.oit.umass.edu [128.119.101.44]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2NEYPlt028124 9, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Mar 2007 09:34:25 -0500 9, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 9, 22 -- (authenticated bits=0) 9, 22 -- by race6.oit.umass.edu (8.13.7/8.13.7) with ESMTP id l2NEYJmJ031997 9, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 9, 22 -- Fri, 23 Mar 2007 10:34:19 -0400 9, 22 -- Message-ID: {4603F38D.1030603-at-research.umass.edu} 9, 22 -- Date: Fri, 23 Mar 2007 10:34:37 -0500 9, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 9, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.2) Gecko/20070222 SeaMonkey/1.1.1 9, 22 -- MIME-Version: 1.0 9, 22 -- To: Tanya.Hayes-at-northampton.ac.uk, 9, 22 -- Microscopy Listserver {Microscopy-at-microscopy.com} 9, 22 -- Subject: Re: [Microscopy] viaWWW: SEM: lines on slow scan/capture 9, 22 -- References: {200703231253.l2NCr8EE001956-at-ns.microscopy.com} 9, 22 -- In-Reply-To: {200703231253.l2NCr8EE001956-at-ns.microscopy.com} 9, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 22 -- Content-Transfer-Encoding: 7bit 9, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Dear Tanya and other Hitachi S3000N owners, I had exactly the same problem on my S3000N last year. Because it persisted through filament and gun changes I knew it wasn't Wehnelt instability. I checked the stage grounding, the BNC cap for the stage ground and used a pure metal, conductive sample and still the problem was there. I checked the objective and final aperture for dirt. I cleaned the column and nothing seemed to help. I noticed the problem did not show up in the BSE imaging, so then I looked at the secondary electron detector itself. When I removed it, the cap over the fluorescent button was a bit loose and the button itself was charging up. I tightened the cap and put a small dab of conductive paint in the corner to make sure there was a path to ground for the electrons that hit the button. I have not seen the problem since. The front of the button has aluminum on it for grounding, but if there is a crack or not good contact between the button and the rest of the SE detector, charge can build up in the button itself, because it is made of a non-conductive plastic. I hope this works for you. Regards,
-----Original Message----- X-from: Tanya.Hayes-at-northampton.ac.uk [mailto:Tanya.Hayes-at-northampton.ac.uk] Sent: March 23, 2007 5:52 AM To: mager-at-interchange.ubc.ca
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Email: Tanya.Hayes-at-northampton.ac.uk Name: Tanya Hayes
Organization: The University of Northampton
Title-Subject: [Filtered] SEM: lines on slow scan/capture
Question: We have an Hitachi S3000N SEM. We have intermittent lines appearing across our images, mainly sourcing in bands from brighter areas, all the way across the image. This has got much worse over the last few weeks. We have ruled out electrical interference & vibrations. Has anyone got any ideas?
Tanya Hayes British School of Leather Technology The University of Northampton Northampton United Kingdom
Well done Mary the give away here is the discharge line will have a little dart on it when it is scintillator discharge. Sure you get white lines just like charging but in this case the little dart is displayed on the lines.
Steve Chapman Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
----- Original Message ----- X-from: {mager-at-interchange.ubc.ca} To: {protrain-at-emcourses.com} Sent: Friday, March 23, 2007 4:04 PM
I like Mary's solution.
I just wanted to mention a few things about the "button".
I assume that this Button is in fact the Scintillator that converts the incoming accelerated SE into light that can then be conveyed to and amplified by the PMT.
At one time the scintillators were made out of fluorescent plastic but they had quite a short service lifetime (100 hrs) and only about 10 hours if used with high beam currents (nano-amps). The problem was the immensely high radiation damage as the incoming SE signal, now accelerated to about 10-15kV, smashed into the outer few microns of the plastic.
Later, manufacturers switched to powdered-phosphor-deposited-on-glass scintillators (usually P-47). This inorganic scintillator gave a much longer service lifetime (1,000s of hours). However, as the powder was an insulator, the deposited powder layer had to be covered with a floated-on carbon or Formvar film and this was then coated with Al to provide conductivity. (Don't put the Al directly onto the powder, it keeps the light from getting out of the grains.). Sometimes the glass-blank below was given a transparent "NESA" coating.
So now the service lifetime limitation became the Al-coated films. They can be damaged by the incoming ionizing radiation and also by mechanical forces associated with vacuum cycling, especially if any air gets behind the film.
The point of this whole rant is that, if the film over the phosphor is "cracked" or otherwise damaged, you will lose a lot of signal. SE may land, but if the surface is negatively charged by previous SE, they will not land with enough energy to make much light and hence the SE signal will seem weak. This can happen so slowly that you don't notice it unless you occasionally calibrate your SE signal from a known clean conducting specimen (Si wafer?), with fixed kV, working distance, etc.
A drop of silver may help but if the paint covers the center of the scintillator where most of the signal arrives, it will prevent the SE from reaching the phosphor and making light. i.e., use the paint sparingly and only around the edge.
Cheers,
Jim P.
********************************************** Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU 3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver Canada Info: http://www.3dcourse.ubc.ca/ Applications still being accepted "If it ain't diffraction, it must be statistics." Anon.
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==============================Original Headers============================== 15, 27 -- From jbpawley-at-wisc.edu Fri Mar 23 13:03:42 2007 15, 27 -- Received: from adsum.doit.wisc.edu (adsum.doit.wisc.edu [144.92.197.210]) 15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2NI3gv4002713 15, 27 -- for {microscopy-at-microscopy.com} ; Fri, 23 Mar 2007 13:03:42 -0500 15, 27 -- Received: from avs-daemon.smtpauth1.wiscmail.wisc.edu by 15, 27 -- smtpauth1.wiscmail.wisc.edu 15, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 15, 27 -- id {0JFD00203BI3MK00-at-smtpauth1.wiscmail.wisc.edu} for 15, 27 -- microscopy-at-microscopy.com; Fri, 23 Mar 2007 13:03:39 -0500 (CDT) 15, 27 -- Received: from [144.92.238.207] by smtpauth1.wiscmail.wisc.edu 15, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 15, 27 -- with ESMTPSA id {0JFD00N4SBI11310-at-smtpauth1.wiscmail.wisc.edu} for 15, 27 -- microscopy-at-microscopy.com; Fri, 23 Mar 2007 13:03:39 -0500 (CDT) 15, 27 -- Date: Fri, 23 Mar 2007 13:03:25 -0500 15, 27 -- From: James Pawley {jbpawley-at-wisc.edu} 15, 27 -- Subject: [Microscopy] RE: viaWWW: SEM: lines on slow scan/capture/ Scintillator? 15, 27 -- In-reply-to: {200703231612.l2NGCD3G019932-at-ns.microscopy.com} 15, 27 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 15, 27 -- Message-id: {p06240854c229c0af3798-at-[144.92.238.207]} 15, 27 -- MIME-version: 1.0 15, 27 -- Content-type: text/plain; charset=us-ascii; format=flowed 15, 27 -- Content-transfer-encoding: 7BIT 15, 27 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=144.92.238.207 15, 27 -- X-Spam-PmxInfo: Server=avs-11, Version=5.3.0.289146, 15, 27 -- Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.3.23.104934, 15, 27 -- SenderIP=144.92.238.207 15, 27 -- References: {200703231612.l2NGCD3G019932-at-ns.microscopy.com} ==============================End of - Headers==============================
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Email: ck-at-ifam.fraunhofer.de Name: Christian K¸bel
Organization: Fraunhofer IFAM
Title-Subject: [Filtered] Re: From PEELS in Tia to EELS in Digital Micrograph
Question: Hi,
It is very simply to transfer EELS data from TIA to DM. Click on the spectrum with the right mouse button, select 'export data' and select the MSA/MAS format. Just open this file in DM and all data including the calibration will be loaded.
Best regards,
Christian K¸bel
----------------------------------------- Dr. Christian Kuebel Head Electron Microscopy
Fraunhofer IFAM Wiener Strasse 12 28359 Bremen Germany
==============================Original Headers============================== 15, 13 -- From zaluzec-at-microscopy.com Sat Mar 24 08:43:13 2007 15, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 15, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2ODhChY005351 15, 13 -- for {microscopy-at-microscopy.com} ; Sat, 24 Mar 2007 08:43:12 -0500 15, 13 -- Mime-Version: 1.0 15, 13 -- Message-Id: {p06240800c22adb614eaa-at-[206.69.208.22]} 15, 13 -- Date: Sat, 24 Mar 2007 08:43:11 -0500 15, 13 -- To: microscopy-at-microscopy.com 15, 13 -- From: ck-at-ifam.fraunhofer.de (by way of MicroscopyListserver) 15, 13 -- Subject: viaWWW: From PEELS in Tia to EELS in Digital Micrograph 15, 13 -- Content-Type: text/plain; charset="iso-8859-1" 15, 13 -- Content-Transfer-Encoding: 8bit 15, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2ODhChY005351 ==============================End of - Headers==============================
The 2007 MAS Topical Workshop on Hyperspectral Imaging (HI-II) has been postponed until October. You can find the new dates on the workshop home page:
http://www.microprobe.org/workshops/HI-II/ {http://www.microprobe.org/workshops/HI-II/} . Pre-registrants were contacted last week informing them of the change, but we realize that there may be individuals who had not pre-registered by might have been planning to attend. Please forward this e-mail to anyone who is not a current member of the society - along with an encouragement to join! - who might have been interested in attending.
As you may be aware, HI-II is to be the first topical workshop to be held at NIST for which MAS has taken the lead. NIST has had a long string of successful workshops, which MAS has been pleased to co-sponsor, and we certainly hope to build on that success while using the venue to promote the values and objectives of MAS. One key strategic goal for the HI-II workshop was that 25% of registrants would be students and postdocs, and a key reason for postponing the workshop was disappointing pre-registration numbers from these early career scientists. The organizers expect that the M&M 2007 meeting will provide a venue to make direct contact with students and their advisors, in order to improve student representation at the October workshop. However, we challenge all MAS members to do their part in promoting MAS and its functions among early career scientists.
Sincerely yours,
Paul Kotula HI-II co-organizer and MAS President -- Senior Electron Microscope Specialist Electron Microscopy Core Facility W136 Veterinary Medicine University of Missouri Columbia, MO 65211-5120 (573) 882-4777, fax 884=2227 email: rosslm-at-missouri.edu http://www.emc.missouri.edu
==============================Original Headers============================== 7, 11 -- From zaluzec-at-microscopy.com Sun Mar 25 17:24:22 2007 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2PMOLCc020740 7, 11 -- for {microscopy-at-microscopy.com} ; Sun, 25 Mar 2007 17:24:22 -0500 7, 11 -- Mime-Version: 1.0 7, 11 -- Message-Id: {p06240802c22ca6e80479-at-[206.69.208.22]} 7, 11 -- Date: Sun, 25 Mar 2007 17:24:20 -0500 7, 11 -- To: microscopy-at-microscopy.com 7, 11 -- From: Lou Ross {RossLM-at-missouri.edu} (by way of MicroscopyListserver) 7, 11 -- Subject: viaWWW: 2007 MAS Topical Workshop Postponed 7, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Hi all, A very quick question, I would like to anneal tantalum and I wonder if there is a big difference between "annealing tantalum under vacuum" and "annealing under argon atmosphere in a tube furnace". I have the second option available and I am not sure if there is still a chance of growing a thin oxide film, other than the native oxide thin film, on top of tantalum surface.
Many thanks in advance
Hany
==============================Original Headers============================== 3, 26 -- From ramadanhany-at-gmail.com Mon Mar 26 16:41:31 2007 3, 26 -- Received: from ug-out-1314.google.com (ug-out-1314.google.com [66.249.92.173]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2QLfScm031861 3, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Mar 2007 16:41:30 -0500 3, 26 -- Received: by ug-out-1314.google.com with SMTP id m2so1809075ugc 3, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Mar 2007 14:41:23 -0700 (PDT) 3, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 3, 26 -- d=gmail.com; s=beta; 3, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 26 -- b=mfh8+t5KLQ2QOJLu7rFy0PbkJ43tCk8DuAnHydBm3Q/1DjdP6V7n+xVdNcjRWj9SNfODPFM91JJAZmXBY3B5eOGtMRa2dHq6od7jbRUyq8h4tLugM92HMDgrp3WLL18m62lyVSvmEfCaA638SThVPd2f7AygAHvx4snCLJSTaAo= 3, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 26 -- d=gmail.com; s=beta; 3, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 26 -- b=PJrqjPMpU1te3lPjLw2Qtg4BvZqJBmKh5V3jv3/HNtfUbtcfEzFGV0gWWCfDbY2+8dNk3Y5dNeoPlubmaz3dCY8BhDhTVojVpnvPfNCaeqWKhaNQ8hejOT8Y2yyY6RJodFyVlBrN07AOkYrqN241hhaao9x3FtdnF+ozAMybxnY= 3, 26 -- Received: by 10.114.166.1 with SMTP id o1mr2845933wae.1174945282853; 3, 26 -- Mon, 26 Mar 2007 14:41:22 -0700 (PDT) 3, 26 -- Received: by 10.114.79.19 with HTTP; Mon, 26 Mar 2007 14:41:22 -0700 (PDT) 3, 26 -- Message-ID: {8d8ce5a30703261441s29084bc4n751d5491539b1f3d-at-mail.gmail.com} 3, 26 -- Date: Mon, 26 Mar 2007 17:41:22 -0400 3, 26 -- From: "Hany Ramadan" {ramadanhany-at-gmail.com} 3, 26 -- To: Microscopy-at-microscopy.com 3, 26 -- Subject: Annealing tantalum......... 3, 26 -- MIME-Version: 1.0 3, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 26 -- Content-Transfer-Encoding: 7bit 3, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
At temperature, tantalum has a very high affinity for oxygen. The accepted practise is to anneal tantalum under vacuum.
First, the tantalum must be clean. It should be degreased, then can be chemically etched with a solution of 60 HNO3, 20 HF and 20 H2SO4.
After the tantalum is vacuum annealed, and the temperature has dropped below 1000°C, the chamber can be backfilled with 15 mm Hg high-purity (99.995%) argon.
It must cool to below 200°C before removing from furnace.
Stu Smalinskas, P.E. Metallurgist SKF USA Plymouth, Michigan
--- ramadanhany-at-gmail.com wrote:
} } Hi all, } A very quick question, I would like to anneal } tantalum and I wonder if } there is a big difference between "annealing } tantalum under vacuum" } and "annealing under argon atmosphere in a tube } furnace". I have the } second option available and I am not sure if there } is still a chance } of growing a thin oxide film, other than the native } oxide thin film, } on top of tantalum surface. } } Many thanks in advance } } Hany }
____________________________________________________________________________________ Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. http://tools.search.yahoo.com/toolbar/features/mail/
==============================Original Headers============================== 11, 20 -- From smalinskas-at-yahoo.com Mon Mar 26 17:57:13 2007 11, 20 -- Received: from web34407.mail.mud.yahoo.com (web34407.mail.mud.yahoo.com [66.163.178.156]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2QMvCG6012193 11, 20 -- for {microscopy-at-sparc5.microscopy.com} ; Mon, 26 Mar 2007 17:57:13 -0500 11, 20 -- Received: (qmail 14022 invoked by uid 60001); 26 Mar 2007 22:57:12 -0000 11, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 20 -- s=s1024; d=yahoo.com; 11, 20 -- h=Message-ID:X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 11, 20 -- b=Cpw9T2bJt59dKpxVmSxlkBIqIffwtzHbjdC2gnbpvwTXteuBTL3vkO2c47NEHiaYjuipul0SMzIURJ3PxMdqgPP9GG6hxfLpIzpDH8R2qDbRzJyuZAiv+AHSIwE0uG7/QFYXo0+m2eeCEJMnJNQfF8jMWmdpIJ7EfIZxK10d4ek= ; 11, 20 -- Message-ID: {20070326225712.14018.qmail-at-web34407.mail.mud.yahoo.com} 11, 20 -- X-YMail-OSG: SIUUgQwVM1nfZj3VkwGE_dFzDCbmHRL5XAYa8eYZbzO0oduVSXAafT04L7dl6Ap.qUEh1dcsaQ5wvUsNzzIa5mFVbBdFMLdlhLqyifaTC4qsPCpG0vhkqDsCmzErqw-- 11, 20 -- Received: from [4.229.150.76] by web34407.mail.mud.yahoo.com via HTTP; Mon, 26 Mar 2007 15:57:12 PDT 11, 20 -- Date: Mon, 26 Mar 2007 15:57:12 -0700 (PDT) 11, 20 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 11, 20 -- Subject: Re: [Microscopy] Annealing tantalum......... 11, 20 -- To: ramadanhany-at-gmail.com, microscopy-at-ns.microscopy.com 11, 20 -- In-Reply-To: {200703262143.l2QLhjRi001923-at-ns.microscopy.com} 11, 20 -- MIME-Version: 1.0 11, 20 -- Content-Type: text/plain; charset=iso-8859-1 11, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Due to expansion of our product portfolio, Carl Zeiss SMT Inc. seeks experienced TEM field service engineers w/apps experience in NY, CA & OH Engineers are responsible for maintenance, defect finding, repair & install of TEMs. A technical degree, Windows & knowledge of physics & vacuum technology is needed. We offer a competitive salary & comprehensive benefits package as well as a promising career opportunity. Send your resume in confidence to: bressan-at-smt.zeiss.com. EOE M/F
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Title-Subject: [Filtered] Applications Engineer Position
Question: Carl Zeiss SMT, the industry leader in e-beam repair products for the mask industry, has an immediate opening for a application specialist in Boise, ID.The sucessful candidate needs an understanding of ebeam technology & a technical bachelors degree. Responsibilities include obtaining market knowledge for all Zeiss SMS ebeam products, supporting customers in their application knowledge, ensuring source & final acceptance of ebeam products,assisting service, managing the escalation process to ensure customer problems are resolved efficiently & assisting in the selling process. Salary is commensurate with experience. Send resume in confidence to: bressan-at-smt.zeiss.com EEO M/F
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Email: Aleksandr.Mironov-at-manchester.ac.uk Name: Aleksandr Mironov
Organization: University of Manchester
Title-Subject: [Filtered] TEM: immunoTEM and BSA
Question: Dear listers,
BSA is often used as a blocking agent in immunoEM protocols (fraction V as I remember). However, there are many kinds of BSA products like: cold ethanol precipitated, heat-shocked processed, IgG free, globulin free, fatty acid free. Are there any differences between them that matter in immunolabelling? Or just buy the cheapest one and it will be OK?
Sincerely,
Aleksandr Mironov Experimental Officer EM Unit, Faculty of Life Sciences University of Manchester M13 9PT UK
We are advertising for a Electron Microscopy Specialist. Please see our ad on the MSA web site at: http://www.microscopy.org/MSAUnits/PlacementOffice/JobListings.html
or go to the University of MN job site link for more details: http://employment.umn.edu/applicants/Central?quickFind=60679
or you can contact me directly for more information.
Mike ****************************************************************** Michael L. Boucher Sr. mboucher-at-tc.umn.edu Lab Manager Rm 18 Office Ph 612-624-6590 I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594 MiNTeC node of the National Nanotechnology Infrastructure Network
University of MN Fax 612-625-5368 12 Shepherd Labs 100 Union Street S.E. Minneapolis, MN 55455 http://www.charfac.umn.edu ********************************************************************
==============================Original Headers============================== 6, 22 -- From mboucher-at-umn.edu Tue Mar 27 08:32:06 2007 6, 22 -- Received: from mta-w2.tc.umn.edu (mta-w2.tc.umn.edu [134.84.119.6]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2RDW5Sq003848 6, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 27 Mar 2007 08:32:05 -0500 6, 22 -- Received: from mike (Mike.charfac.umn.edu [160.94.16.142]) 6, 22 -- by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP 6, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 27 Mar 2007 08:32:03 -0500 (CDT) 6, 22 -- X-Umn-Remote-Mta: [N] Mike.charfac.umn.edu [160.94.16.142] #+LO+TS+AU+HN 6, 22 -- Reply-To: {mboucher-at-umn.edu} 6, 22 -- From: "Michael L. Boucher" {mboucher-at-umn.edu} 6, 22 -- To: {Microscopy-at-Microscopy.Com} 6, 22 -- Subject: Position open: Electron Microscopy Specialist 6, 22 -- Date: Tue, 27 Mar 2007 08:32:25 -0500 6, 22 -- Organization: University of MN 6, 22 -- Message-ID: {004d01c77074$5bb479a0$8e105ea0-at-charfac.umn.edu} 6, 22 -- MIME-Version: 1.0 6, 22 -- Content-Type: text/plain; 6, 22 -- charset="US-ASCII" 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-Mailer: Microsoft Office Outlook 11 6, 22 -- Thread-Index: AcdwdFtjYLkbMig3Q5aNSfztAAcpxw== 6, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 ==============================End of - Headers==============================
Let me start by admitting I have not actually done a rigorous test of this, but I generally buy the IgG free version for all my immunocytochemistry work. I do a lot of immuno-staining of 0.5 um semi-thick sections on glass slides and a fair amount of EM grids and a bottle lasts a long time. If I was doing several Western blots a day, I would be more concerned with cost. If you look at the specifications of many "high quality" BSA's, they are 97-99% pure. That is a great level of purity for many applications but the 1-3% impurities are generally immunoglobulin. IgG is 15% of the protein in human serum. If you are using secondary antibodies that don't cross react with bovine IgG, it might be unimportant. If you are using protein A or protein G, it could start to be significant. Good luck, Tom
At 08:27 AM 03/27/07, you wrote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
I would like to get in contact with someone who has experience using a vibratome.
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 5, 20 -- From tbargar-at-unmc.edu Tue Mar 27 14:54:47 2007 5, 20 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2RJsk7g011568 5, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 27 Mar 2007 14:54:47 -0500 5, 20 -- Received: from zixvpm01.unmc.edu (ZixVPM [127.0.0.1]) 5, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id C76614C07E 5, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 27 Mar 2007 14:54:46 -0500 (CDT) 5, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu [137.197.103.35]) 5, 20 -- by zixvpm01.unmc.edu (Proprietary) with ESMTP id 98B0C4C06D 5, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 27 Mar 2007 14:54:45 -0500 (CDT) 5, 20 -- Subject: vibratome use 5, 20 -- To: Microscopy-at-MSA.Microscopy.com 5, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 5, 20 -- Message-ID: {OF5607B500.DF3D7881-ON862572AB.006D226C-862572AB.006D617C-at-unmc.edu} 5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 5, 20 -- Date: Tue, 27 Mar 2007 14:54:44 -0500 5, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 03/27/2007 02:54:45 5, 20 -- PM 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Energy Beam Sciences is a distributor of the Vibratome line of equipment, if you contact me I'm sure we can help,and/or put you in contact with a user.
Mike Dufraine
tbargar-at-unmc.edu wrote:
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==============================Original Headers============================== 7, 27 -- From mdufraine-at-ebsciences.com Tue Mar 27 15:10:34 2007 7, 27 -- Received: from mx-outbound01.easydns.com (mailout.easydns.com [205.210.42.54]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2RKAYNN023420 7, 27 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 27 Mar 2007 15:10:34 -0500 7, 27 -- Received: from mail.ebsciences.com (unknown [69.182.224.2]) 7, 27 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 7, 27 -- (No client certificate requested) 7, 27 -- by mx-outbound01.easydns.com (Postfix) with ESMTP id 3B87F7FB0; 7, 27 -- Tue, 27 Mar 2007 16:08:23 -0400 (EDT) 7, 27 -- Received: from mdufraine.ebsciences.private ([10.10.0.195]) 7, 27 -- by mail.ebsciences.com with esmtpsa (TLSv1:AES256-SHA:256) 7, 27 -- (Exim 4.62) 7, 27 -- (envelope-from {mdufraine-at-ebsciences.com} ) 7, 27 -- id 1HWI00-0000rU-T3; Tue, 27 Mar 2007 15:10:32 -0500 7, 27 -- Message-ID: {46097A37.50700-at-ebsciences.com} 7, 27 -- Date: Tue, 27 Mar 2007 16:10:31 -0400 7, 27 -- From: Mike Dufraine {mdufraine-at-ebsciences.com} 7, 27 -- Organization: Energy Beam Sciences 7, 27 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 7, 27 -- X-Accept-Language: en-us, en 7, 27 -- MIME-Version: 1.0 7, 27 -- To: tbargar-at-unmc.edu, Microscopy-at-MSA.Microscopy.com 7, 27 -- Subject: Re: [Microscopy] vibratome use 7, 27 -- References: {200703271955.l2RJtrlt012754-at-ns.microscopy.com} 7, 27 -- In-Reply-To: {200703271955.l2RJtrlt012754-at-ns.microscopy.com} 7, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
What do you need, Tom? I have used the vibratome quite a bit and can offer some general help but it would help if you can be more specific.
Roger Moretz, Ph.D. Dept. of Toxicology Boehringer Ingelheim Pharmaceuticals, Inc. Ridgefield, CT
On 3/27/07, tbargar-at-unmc.edu {tbargar-at-unmc.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } I would like to get in contact with someone who has experience using a } vibratome. } } } Tom Bargar } University of Nebraska Medical Center } Core Electron Microscopy Research Facility } 986395 Nebraska Medical Center } Omaha, NE 68198-6395 } 402-559-7347 } tbargar-at-unmc.edu } } } ==============================Original Headers============================== } 5, 20 -- From tbargar-at-unmc.edu Tue Mar 27 14:54:47 2007 } 5, 20 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) } 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2RJsk7g011568 } 5, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 27 Mar 2007 14:54:47 -0500 } 5, 20 -- Received: from zixvpm01.unmc.edu (ZixVPM [127.0.0.1]) } 5, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id C76614C07E } 5, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 27 Mar 2007 14:54:46 -0500 (CDT) } 5, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu [137.197.103.35]) } 5, 20 -- by zixvpm01.unmc.edu (Proprietary) with ESMTP id 98B0C4C06D } 5, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 27 Mar 2007 14:54:45 -0500 (CDT) } 5, 20 -- Subject: vibratome use } 5, 20 -- To: Microscopy-at-MSA.Microscopy.com } 5, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 } 5, 20 -- Message-ID: {OF5607B500.DF3D7881-ON862572AB.006D226C-862572AB.006D617C-at-unmc.edu} } 5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} } 5, 20 -- Date: Tue, 27 Mar 2007 14:54:44 -0500 } 5, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 03/27/2007 02:54:45 } 5, 20 -- PM } 5, 20 -- MIME-Version: 1.0 } 5, 20 -- Content-type: text/plain; charset=US-ASCII } ==============================End of - Headers============================== }
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OK, so I am lazy and want to mine the group before doing it the hard way.
I have an old Wild M5A that had a Wild film camera on it. Now everyone wants to use their digital camera in place of the old film camera.
I have resisted getting a digital set up because the market was so fluid. I tried once, but by the time I figured everything out, the camera was obsolete and I needed a new computer to run it anyway.
So now I have a user who has a nice Nikon digital SLR who wants to hook it up to the Wild.
I need some guidance helping her find the right adapters. She got a T-mount, but that only goes halfway to the solution. The camera tube on the Wild is something like 35 mm diameter. I have a C-mount adapter for a video camera that fits this scope. What do I need to get her SLR to work?
Thanks
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I am riding in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. Visit http://www.aidslifecycle.org for more information about the ride or http://www.aidslifecycle.org/5482 to make a donation.
==============================Original Headers============================== 10, 17 -- From jmkrupp-at-ucsc.edu Tue Mar 27 17:46:46 2007 10, 17 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2RMkjlb017038 10, 17 -- for {microscopy-at-microscopy.com} ; Tue, 27 Mar 2007 17:46:45 -0500 10, 17 -- Received: from [128.114.125.5] (HELO ucsc.edu) 10, 17 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.0.9) 10, 17 -- with ESMTPS id 12668099 for microscopy-at-microscopy.com; Tue, 27 Mar 2007 15:46:41 -0700 10, 17 -- Received: from [128.114.25.217] (account jmkrupp-at-ucsc.edu HELO [128.114.25.217]) 10, 17 -- by copper.ucsc.edu (CommuniGate Pro SMTP 4.3.7) 10, 17 -- with ESMTPA id 112377697 for microscopy-at-microscopy.com; Tue, 27 Mar 2007 15:46:40 -0700 10, 17 -- Mime-Version: 1.0 10, 17 -- Message-Id: {p06230902c22f4d0f1edb-at-[128.114.25.217]} 10, 17 -- Date: Tue, 27 Mar 2007 15:46:39 -0700 10, 17 -- To: microscopy-at-microscopy.com 10, 17 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 10, 17 -- Subject: Digital camera to stereoscope 10, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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Question: This is a key position within our organization. The individual will be responsible primarily for providing application support to showcase our complete line of EDS (Microanalysis) and Energy Dispersive X-ray Fluorescence (EDXRF) products. Prior experience using EDS, SEM and EDXRF techniques as well as other metrology techniques will be important. The prime roles will include generating application data and reports to be used in promoting our EDS and X-ray systems, working with customers and the local sales engineer to provide proof data to enable funding justification and generating novel application ideas for utilizing our EDS and X-ray technology. It is also expected that the individual will aide in visiting potential customers with the sales engineer to review technical requirements related to the purchasing of our product, along with performing demos at the customer site or demo facility. Contribute to providing scientific presentations in collaboration with our customers as well as on their own at seminars and conferences. A minimum of a BS in Scientific Field, 2 or more years experience in customer support is required although a applicant with an Associates Degree and 5 years experience in the SEM and XRF field will be considered. This position requires outstanding written and oral communication skills. Excellent software skills and knowledge of Microsoft Office as well as Statistical Control packages is expected.
Virginia Tech is seeking an Instrument specialist for its new Nanoscale Characterization and Fabrication Laboratory. The selected candidate will: Maintain and operate the X-ray Photoelectron Spectrometer (XPS) and Secondary Ion Mass Spectrometer (SIMS) in the NCFL. Keep the laboratory up to date in the methods and techniques used with these instruments. Perform TEM and FIB analyses and provide assessments of the results, support individual research with training on the instruments and guidance on the techniques. Provide analytical service to industrial clients.
For more information or to apply, go to www.jobs.vt.edu, posting number 070280. Complete the faculty application and attach a cover letter, resume or CV, and a list of professional references. Individuals desiring accommodation in the application process should notify Christie Thompson, cthomp-at-vt.edu or 540-231-5495. Review of applications will begin on April 5, 2007.
Stephen McCartney Senior Research Associate Macromolecules and Interfaces Institute 2108 Hahn Hall Va Tech Blacksburg, VA 24061 540-231-9765 - phone 540-231-8517 - FAX
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We have a poster coming up at M&M 2007 for this very thing . . .
But you want an answer now. The Nikon: I assume this is one of the SLR's (i.e. D50, D80, D100, D200?). Note the D50 shakes due to shutter/mirror slap and will be a problem at higher mags. Get the Nikon software and USB cable, run directly through the computer (rather than collecting on flashcard) - well worth the $100.
You are right you need to use the 35mm mount actually an F-mount (not T-mount) You can get a T- to Nikon (F-) adapter at a Photo shop BHPhoto.com has them ~ $15-20 get a better one.
OR you need the mount from the photo port on the Wild to F-mount? Do you have any Photoeyepieces?
Unless you can find the 35-mm camera parts in a drawer somewhere or someone else on the list getting Wild parts will be hard. However, Diagnostics seems to make pretty good couplers.
On 27 Mar 2007 at 17:47, jmkrupp-at-ucsc.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi: } } OK, so I am lazy and want to mine the group before doing it the hard way. } } I have an old Wild M5A that had a Wild film camera on it. Now } everyone wants to use their digital camera in place of the old film } camera. } } I have resisted getting a digital set up because the market was so } fluid. I tried once, but by the time I figured everything out, the } camera was obsolete and I needed a new computer to run it anyway. } } So now I have a user who has a nice Nikon digital SLR who wants to } hook it up to the Wild. } } I need some guidance helping her find the right adapters. She got a } T-mount, but that only goes halfway to the solution. The camera tube } on the Wild is something like 35 mm diameter. I have a C-mount } adapter for a video camera that fits this scope. What do I need to } get her SLR to work? } } Thanks } } Jon } -- } } Jonathan Krupp } Microscopy & Imaging Lab } C230 Earth & Marine Science } University of California } Santa Cruz, CA 95064 } (831) 459-2477 } jmkrupp-at-ucsc.edu } } I am riding in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise } money for the San Francisco AIDS Foundation. Visit } http://www.aidslifecycle.org for more information about the ride or } http://www.aidslifecycle.org/5482 to make a donation. } } ==============================Original Headers============================== } 10, 17 -- From jmkrupp-at-ucsc.edu Tue Mar 27 17:46:46 2007 } 10, 17 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) } 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2RMkjlb017038 } 10, 17 -- for {microscopy-at-microscopy.com} ; Tue, 27 Mar 2007 17:46:45 -0500 } 10, 17 -- Received: from [128.114.125.5] (HELO ucsc.edu) } 10, 17 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.0.9) } 10, 17 -- with ESMTPS id 12668099 for microscopy-at-microscopy.com; Tue, 27 Mar 2007 15:46:41 -0700 } 10, 17 -- Received: from [128.114.25.217] (account jmkrupp-at-ucsc.edu HELO [128.114.25.217]) } 10, 17 -- by copper.ucsc.edu (CommuniGate Pro SMTP 4.3.7) } 10, 17 -- with ESMTPA id 112377697 for microscopy-at-microscopy.com; Tue, 27 Mar 2007 15:46:40 -0700 } 10, 17 -- Mime-Version: 1.0 } 10, 17 -- Message-Id: {p06230902c22f4d0f1edb-at-[128.114.25.217]} } 10, 17 -- Date: Tue, 27 Mar 2007 15:46:39 -0700 } 10, 17 -- To: microscopy-at-microscopy.com } 10, 17 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} } 10, 17 -- Subject: Digital camera to stereoscope } 10, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 15, 30 -- From edelmare-at-muohio.edu Wed Mar 28 15:14:54 2007 15, 30 -- Received: from spamfirewall.muohio.edu (wallaby.mcs.muohio.edu [134.53.6.28]) 15, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2SKEsea025834 15, 30 -- for {microscopy-at-Microscopy.com} ; Wed, 28 Mar 2007 15:14:54 -0500 15, 30 -- X-ASG-Debug-ID: 1175112892-7bc800360000-Dem1zR 15, 30 -- X-Barracuda-URL: http://spamfirewall.muohio.edu:80/cgi-bin/mark.cgi 15, 30 -- X-Barracuda-Connect: mulnx23.mcs.muohio.edu[134.53.6.10] 15, 30 -- X-Barracuda-Start-Time: 1175112892 15, 30 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 15, 30 -- by spamfirewall.muohio.edu (Spam Firewall) with ESMTP 15, 30 -- id DE4C7241B84; Wed, 28 Mar 2007 16:14:52 -0400 (EDT) 15, 30 -- Received: from [192.168.1.23] ([134.53.14.105]) 15, 30 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l2SKEqmD023385; 15, 30 -- Wed, 28 Mar 2007 16:14:52 -0400 15, 30 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 15, 30 -- To: jmkrupp-at-ucsc.edu 15, 30 -- Date: Wed, 28 Mar 2007 16:14:53 -0400 15, 30 -- MIME-Version: 1.0 15, 30 -- X-ASG-Orig-Subj: Re: [Microscopy] Digital camera to stereoscope 15, 30 -- Subject: Re: [Microscopy] Digital camera to stereoscope 15, 30 -- CC: microscopy-at-Microscopy.com 15, 30 -- Message-ID: {460A947D.7573.2876152-at-edelmare.muohio.edu} 15, 30 -- Priority: normal 15, 30 -- In-reply-to: {200703272247.l2RMlb1M018297-at-ns.microscopy.com} 15, 30 -- References: {200703272247.l2RMlb1M018297-at-ns.microscopy.com} 15, 30 -- X-mailer: Pegasus Mail for Windows (4.41) 15, 30 -- Content-type: text/plain; charset=US-ASCII 15, 30 -- Content-transfer-encoding: 7BIT 15, 30 -- Content-description: Mail message body 15, 30 -- X-Barracuda-Virus-Scanned: by Barracuda Spam Firewall at muohio.edu ==============================End of - Headers==============================
Also, you want to remember that YOU see stereo because of your brain combining two images which come from a separation of approximately 12-15 degrees. Please warn your user that the image collected through the photographic system will only be "mono", not stereo. This difference in viewpoint causes a lot of frustration for folks beginning photography through a stereo microscope, whether it be to film or digital format.
Hope this was helpful!
Barbara Foster, President
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
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P. S. Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 02:20 PM 3/28/2007, edelmare-at-muohio.edu wrote:
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I don't know whether the D80 moves or not, but as a camera, it's a lot better than the D70.
We found that people found the D70 unusable not just because the shutter/mirror made the microscope shake, but because people just can't focus.
They much prefer the lower resolution Axiocam that they can focus on the screen. For most users, convenience trumps quality.
-Michael
At 03:16 PM 03/28/07, you wrote: } SLR's (i.e. D50, D80, D100, D200?). Note the D50 shakes due to } shutter/mirror slap and will be a problem at higher mags. Get the
____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. URL: http://www.aecom.yu.edu/aif/
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Email: chumbley-at-iastate.edu Name: Scott Chumbley
Organization: Iowa State University
Title-Subject: [Filtered] Looking for used TEM
Question: Does anyone have a used Philips / FEI CM-type TEM they are looking to get rid of? ISU is looking to buy a CM12, CM20 or CM30 in case somebody has upgraded to a newer instrument and is looking to sell their older microscope.
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Email: hfong11-at-yahoo.com Name: Hanson Fong
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Title-Subject: [Filtered] Parts for TEM and Ion Mill
Question: The Department of Materials Sci & Engineering at University of Washington is looking for the following parts: 1. single tilt holder for Phillips EM420 (1 or more) 2. a diffusion pump for a Gatan Model 600 Dual Ion Mill
If someone has these parts in good working condition and is willing to donate or sell at a reasonable price, please contact Hanson Fong at:
hfong-at-u.washington.edu or hfong11-at-yahoo.com (preferred)
Thank you, Hanson Fong, Ph. D. University of Washington Materials Sci & Eng 302 Roberts Hall Box 352120 Seattle, WA 98195
This Question was submitted to Ask-A-Microscopist by (dang-at-oakland.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, March 28, 2007 at 14:20:58 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both dang-at-oakland.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: dang-at-oakland.edu Name: LOAN DANG
Organization: Oakland University
Education: Graduate College
Location: Rochester Hills michigan 48309
Title: To obtain the morphology under Confocal Microscope
Question: I have sample (cartilage sample) I would like to see the structure under Confocal Microscope . Do I need to stain the sample in order to see it . I really appreciate your response, Sincerely LOAN DANG
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==============================Original Headers============================== 6, 20 -- From MCarlyle-at-veeco.com Wed Mar 28 18:37:38 2007 6, 20 -- Received: from sboexch2.int.veeco.com (SBOEXCH2.VEECO.com [68.111.35.167]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2SNbcxL002589 6, 20 -- for {Microscopy-at-Microscopy.com} ; Wed, 28 Mar 2007 18:37:38 -0500 6, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 6, 20 -- content-class: urn:content-classes:message 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="us-ascii" 6, 20 -- Subject: SPM - Seeing at the Nanoscale conference 6, 20 -- Date: Wed, 28 Mar 2007 16:37:38 -0700 6, 20 -- Message-ID: {625BD6DD96DE554DBB0558FFE70E0F420225C6DC-at-sboexch2.int.veeco.com} 6, 20 -- X-MS-Has-Attach: 6, 20 -- X-MS-TNEF-Correlator: 6, 20 -- Thread-Topic: SPM - Seeing at the Nanoscale conference 6, 20 -- Thread-Index: AcdxkhMFO1vDMa0MQN2Y50ls9TRzrA== 6, 20 -- From: "Marlene Carlyle" {MCarlyle-at-veeco.com} 6, 20 -- To: {Microscopy-at-Microscopy.com} 6, 20 -- Content-Transfer-Encoding: 8bit 6, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2SNbcxL002589 ==============================End of - Headers==============================
I have been given a protocol for embedding Cyanobacterium synechocystis from liquid culture, after secondary fixation the cells are embedded in 2% agar prior to dehydration and resin embedding. When I have tried this method before the agar has set so rapidly I have had insufficient time to incorporate the cell suspension evenly within the agar. Details of the type of agar, temperatures and method of incorporation are absent so any help on the practicalities of achieving a fairly uniform distribution of cells would be gratefully received. I usually work with the cell suspensions in Eppendorf (micro-centrifuge) tubes. Thanks in advance.
Carol Evered Research Imaging Warwick HRI University of Warwick Wellesbourne Warwick CV35 9EF UK
==============================Original Headers============================== 1, 26 -- From Carol.Evered-at-warwick.ac.uk Thu Mar 29 03:48:04 2007 1, 26 -- Received: from mail-relay-1.csv.warwick.ac.uk (mail-relay-1.warwick.ac.uk [137.205.128.7]) 1, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2T8m3nA025426 1, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 03:48:04 -0500 1, 26 -- Received: from localhost (localhost [127.0.0.1]) 1, 26 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id l2T8m2Xo012504 1, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 09:48:02 +0100 (BST) 1, 26 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 1, 26 -- Received: from mail-relay-1.csv.warwick.ac.uk ([127.0.0.1]) 1, 26 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 1, 26 -- with LMTP id O0MeNzfCO5AF for {Microscopy-at-microscopy.com} ; 1, 26 -- Thu, 29 Mar 2007 09:48:02 +0100 (BST) 1, 26 -- Received: from ntsw02.hri.warwick.ac.uk (f5-snat-dflt [137.205.195.103]) 1, 26 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id l2T8lwbf012424 1, 26 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 Mar 2007 09:47:58 +0100 (BST) 1, 26 -- X-Envelope-From: Carol.Evered-at-hri.ac.uk 1, 26 -- Received: by ntsw02.hri.warwick.ac.uk with Internet Mail Service (5.5.2653.19) 1, 26 -- id {G8TFL3V9} ; Thu, 29 Mar 2007 09:52:38 +0100 1, 26 -- Message-ID: {AE626396FFEDD81199A7000E7FACF9E617E224-at-ntsw14.hri.warwick.ac.uk} 1, 26 -- From: "Evered, Carol" {Carol.Evered-at-warwick.ac.uk} 1, 26 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-microscopy.com} 1, 26 -- Subject: TEM help with pre-embedding cells in agar 1, 26 -- Date: Thu, 29 Mar 2007 09:48:27 +0100 1, 26 -- MIME-Version: 1.0 1, 26 -- X-Mailer: Internet Mail Service (5.5.2653.19) 1, 26 -- Content-Type: text/plain ==============================End of - Headers==============================
I have normally just embedded a spun down pellet in agar so the problem of dispersing is not normally an issue.
I have read however of a method that involves: 1. melt 2% agar and then store in 50 deg C water bath 2. take a fixed and washed pellet (in eppendorf) and warm in 50 deg C water bath 3. add warm agar to pellet and re-suspend 4. then leave for 5 mins in water bath 5. spin down rapidly 30 secs to 1 min - any longer and agar may set too soon. I am not sure if you want to keep yours dispersed though. 6. cool in refrigerator or ice bath 7. chop up agar as required
The original method is: Hirsch JG & Fedorko ME (1968), Journal of Cell Biology 38:615.
But is cited in a large double volume: Procedures in Electron Microscopy A.W. Robards & A.J. Wilson (editors) Pub 1993 John Wiley ISBN 0 471 92853 4 pages 5:9.3-4
There are different melting point agars so it might be possible to experiment with temperatures or you could even try acrylamide gels which are cold setting.
Hope this helps.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: Carol.Evered-at-warwick.ac.uk
Let me float an question to the list. What is the effect of changing a one meg camera to a three meg camera on a TEM?
I'm asked some fundamental questions, and I can't seem to answer them to this person's satisfaction, which implies I don't have a good grasp of the question or answers.
My immediate response is you would increase the resolution. I can envision the image size on the monitor changing, but if the resolution of the screen is lower than the captured image and if the computer/imaging software wants to display all the image captured, will not any feature at a specific magnification have the resolution of the monitor? It seems the same is true for the printer. I can't simply expand the size of the paper at will, so the software will either reduce the printed image magnification or print just a smaller section of the total image. Again, since the printer has a fixed resolution will not the printed image resolution will be limited by the printer's (This sounds like a Hi-Fi discussion from the early 60's... just change the words...) upper limit?
So why capture high resolution images? My response is it allows you to post process and expand the image to examine one feature and have sufficient "stored" resolution to display the image without empty magnification. This also implies (to me at least) if I want to measure from point A to point B, the more camera pixels I have the better I can resolve where point A starts and point B ends which should allow me to have better confidence in my measurements. I believe that imaging software works on the image in memory and not the image displayed on the screen so size of the print or screen has little to do with data obtained. It's more a function of the size of the captured image?
Lastly.....
If I feel the need to have at least 1000 pixels in an image feature, and due to my camera I can only capture 500 at a magnification X, is there any reason not to simply increase the magnification so I have a larger feature which now occupies more pixels. I realize I haven't increased the resolution, but if my software need 999 pixels to "recognize" a feature haven't I met this requirement?
Thank in advance! Frank (I miss film) Karl
==============================Original Headers============================== 10, 17 -- From frank.karl-at-degussa.com Thu Mar 29 07:42:34 2007 10, 17 -- Received: from framailout1.rz.itson.com (mailout2.degussa.com [149.216.91.173]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2TCgXHD021519 10, 17 -- for {microscopy-at-msa.microscopy.com} ; Thu, 29 Mar 2007 07:42:33 -0500 10, 17 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [10.88.88.98]) 10, 17 -- by framailout1.rz.itson.com (8.13.4/8.13.4/Debian-3sarge3) with ESMTP id l2TCg2Bb031526 10, 17 -- for {microscopy-at-msa.microscopy.com} ; Thu, 29 Mar 2007 14:42:29 +0200 10, 17 -- Subject: Digital cameras and the TEM 10, 17 -- To: microscopy-at-msa.microscopy.com 10, 17 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 10, 17 -- Message-ID: {OF3BDC677E.DA558E0C-ON862572AD.003F9EC7-852572AD.0045C6B9-at-degussa.com} 10, 17 -- From: frank.karl-at-degussa.com 10, 17 -- Date: Thu, 29 Mar 2007 08:42:21 -0400 10, 17 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 10, 17 -- 03/29/2007 07:42:30 AM 10, 17 -- MIME-Version: 1.0 10, 17 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
You should try a low temperature gelling agarose. We use Sigma Type VII regularly. Keep the agarose at about 40oC until needed. Fix the cells as desired while in suspension. Use the agarose as the last step prior to dehydration so you are sure all cells are fully exposed to fix and washing. Spin the cells down in the Eppendorf tube and remove the supernatant. Then add ~0.5 ml agarose. Gently stir the cells up a little to get the agarose to enrobe them while keeping the tubes in warm water so the agarose remains liquid. Spin and the cells should have plenty of time to pellet again before the agarose sets.
Trick is to not try to resuspend the cells completely in the agarose or the ones at the top will not have time to get to the bottom before the agarose sets up.
We then cool the tubes in ice (or very cool water) for a few minutes. Inject water or low percentage ETOH toward the bottom of the tube by slipping the pipette down the side of the agarose plug. If you do this gently you can get the plug to release and float up so that it can be dumped out and then the pellet sliced into appropriate sized pieces for dehydration and infiltration.
Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
} From: {Carol.Evered-at-warwick.ac.uk} } Reply-To: {Carol.Evered-at-warwick.ac.uk} } Date: Thu, 29 Mar 2007 03:50:39 -0500 } To: {dsherman-at-purdue.edu} } Subject: [Microscopy] TEM help with pre-embedding cells in agar } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I have been given a protocol for embedding Cyanobacterium synechocystis from } liquid culture, after secondary fixation the cells are embedded in 2% agar } prior to dehydration and resin embedding. } When I have tried this method before the agar has set so rapidly I have had } insufficient time to incorporate the cell suspension evenly within the agar. } Details of the type of agar, temperatures and method of incorporation are } absent so any help on the practicalities of achieving a fairly uniform } distribution of cells would be gratefully received. I usually work with the } cell suspensions in Eppendorf (micro-centrifuge) tubes. } Thanks in advance. } } Carol Evered } Research Imaging } Warwick HRI } University of Warwick } Wellesbourne } Warwick } CV35 9EF } UK } } ==============================Original Headers============================== } 1, 26 -- From Carol.Evered-at-warwick.ac.uk Thu Mar 29 03:48:04 2007 } 1, 26 -- Received: from mail-relay-1.csv.warwick.ac.uk } (mail-relay-1.warwick.ac.uk [137.205.128.7]) } 1, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l2T8m3nA025426 } 1, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 03:48:04 -0500 } 1, 26 -- Received: from localhost (localhost [127.0.0.1]) } 1, 26 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id } l2T8m2Xo012504 } 1, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 09:48:02 +0100 } (BST) } 1, 26 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk } 1, 26 -- Received: from mail-relay-1.csv.warwick.ac.uk ([127.0.0.1]) } 1, 26 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) } 1, 26 -- with LMTP id O0MeNzfCO5AF for {Microscopy-at-microscopy.com} ; } 1, 26 -- Thu, 29 Mar 2007 09:48:02 +0100 (BST) } 1, 26 -- Received: from ntsw02.hri.warwick.ac.uk (f5-snat-dflt } [137.205.195.103]) } 1, 26 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id } l2T8lwbf012424 } 1, 26 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 Mar 2007 09:47:58 +0100 } (BST) } 1, 26 -- X-Envelope-From: Carol.Evered-at-hri.ac.uk } 1, 26 -- Received: by ntsw02.hri.warwick.ac.uk with Internet Mail Service } (5.5.2653.19) } 1, 26 -- id {G8TFL3V9} ; Thu, 29 Mar 2007 09:52:38 +0100 } 1, 26 -- Message-ID: } {AE626396FFEDD81199A7000E7FACF9E617E224-at-ntsw14.hri.warwick.ac.uk} } 1, 26 -- From: "Evered, Carol" {Carol.Evered-at-warwick.ac.uk} } 1, 26 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-microscopy.com} } 1, 26 -- Subject: TEM help with pre-embedding cells in agar } 1, 26 -- Date: Thu, 29 Mar 2007 09:48:27 +0100 } 1, 26 -- MIME-Version: 1.0 } 1, 26 -- X-Mailer: Internet Mail Service (5.5.2653.19) } 1, 26 -- Content-Type: text/plain } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 23 -- From dsherman-at-purdue.edu Thu Mar 29 08:55:34 2007 9, 23 -- Received: from 1061exfe03.adpc.purdue.edu (1061exfe03.adpc.purdue.edu [128.210.63.225]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2TDtY5H001921 9, 23 -- for {microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 08:55:34 -0500 9, 23 -- Received: from exchange.purdue.edu ([172.21.6.23]) by 1061exfe03.adpc.purdue.edu with Microsoft SMTPSVC(6.0.3790.211); 9, 23 -- Thu, 29 Mar 2007 09:55:34 -0400 9, 23 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 9, 23 -- Thu, 29 Mar 2007 13:55:33 +0000 9, 23 -- User-Agent: Microsoft-Entourage/11.3.3.061214 9, 23 -- Date: Thu, 29 Mar 2007 09:55:32 -0400 9, 23 -- Subject: Re: [Microscopy] TEM help with pre-embedding cells in agar 9, 23 -- From: Debby Sherman {dsherman-at-purdue.edu} 9, 23 -- To: {Carol.Evered-at-warwick.ac.uk} , 9, 23 -- "message to: MSA list" {microscopy-at-microscopy.com} 9, 23 -- Message-ID: {C2313D94.1A75F%dsherman-at-purdue.edu} 9, 23 -- Thread-Topic: [Microscopy] TEM help with pre-embedding cells in agar 9, 23 -- Thread-Index: AcdyCeqgKVDwUN39EduLfQARJN08Mg== 9, 23 -- In-Reply-To: {200703290850.l2T8odpT029189-at-ns.microscopy.com} 9, 23 -- Mime-version: 1.0 9, 23 -- Content-type: text/plain; 9, 23 -- charset="US-ASCII" 9, 23 -- Content-transfer-encoding: 7bit 9, 23 -- X-OriginalArrivalTime: 29 Mar 2007 13:55:34.0049 (UTC) FILETIME=[EBD90910:01C77209] ==============================End of - Headers==============================
I have done this many times. I use Low Melting point Agarose. Heat the Eppendorph tube (with the bugs in it) up to 37-40°C. Mix your bugs and agarose in the warm tube. Once the bugs are mixed (pipet up and down a few times) then put the sample on ice to cool the bugs and harden the agarose. If you need to, you can heat the tube more, which will give you more time to work until the agarose hardens. If you work fast and ice the sample immediately after working then the increased temp has not caused me problems.
FYI I have used the same technique for many kinds of suspended cells.
I have found this to work well.
David
_____________________
David Elliott Ph.D. Assistant Professor - Department of Cell Biology and Anatomy Director, Research Microscopy Core Service University of Arizona College of Medicine PO Box 245004 Tucson, AZ 85724
Voice: 520-626-7870 Fax: 520-626-2097
On Mar 29, 2007, at 1:51 AM, Carol.Evered-at-warwick.ac.uk wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } I have been given a protocol for embedding Cyanobacterium } synechocystis from } liquid culture, after secondary fixation the cells are embedded in } 2% agar } prior to dehydration and resin embedding. } When I have tried this method before the agar has set so rapidly I } have had } insufficient time to incorporate the cell suspension evenly within } the agar. } Details of the type of agar, temperatures and method of } incorporation are } absent so any help on the practicalities of achieving a fairly uniform } distribution of cells would be gratefully received. I usually work } with the } cell suspensions in Eppendorf (micro-centrifuge) tubes. } Thanks in advance. } } Carol Evered } Research Imaging } Warwick HRI } University of Warwick } Wellesbourne } Warwick } CV35 9EF } UK } } ==============================Original } Headers============================== } 1, 26 -- From Carol.Evered-at-warwick.ac.uk Thu Mar 29 03:48:04 2007 } 1, 26 -- Received: from mail-relay-1.csv.warwick.ac.uk (mail- } relay-1.warwick.ac.uk [137.205.128.7]) } 1, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id l2T8m3nA025426 } 1, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 } 03:48:04 -0500 } 1, 26 -- Received: from localhost (localhost [127.0.0.1]) } 1, 26 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with } ESMTP id l2T8m2Xo012504 } 1, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 } 09:48:02 +0100 (BST) } 1, 26 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk } 1, 26 -- Received: from mail-relay-1.csv.warwick.ac.uk ([127.0.0.1]) } 1, 26 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port } 10024) } 1, 26 -- with LMTP id O0MeNzfCO5AF for {Microscopy-at-microscopy.com} ; } 1, 26 -- Thu, 29 Mar 2007 09:48:02 +0100 (BST) } 1, 26 -- Received: from ntsw02.hri.warwick.ac.uk (f5-snat-dflt } [137.205.195.103]) } 1, 26 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with } ESMTP id l2T8lwbf012424 } 1, 26 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 Mar 2007 } 09:47:58 +0100 (BST) } 1, 26 -- X-Envelope-From: Carol.Evered-at-hri.ac.uk } 1, 26 -- Received: by ntsw02.hri.warwick.ac.uk with Internet Mail } Service (5.5.2653.19) } 1, 26 -- id {G8TFL3V9} ; Thu, 29 Mar 2007 09:52:38 +0100 } 1, 26 -- Message-ID: } {AE626396FFEDD81199A7000E7FACF9E617E224-at-ntsw14.hri.warwick.ac.uk} } 1, 26 -- From: "Evered, Carol" {Carol.Evered-at-warwick.ac.uk} } 1, 26 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-microscopy.com} } 1, 26 -- Subject: TEM help with pre-embedding cells in agar } 1, 26 -- Date: Thu, 29 Mar 2007 09:48:27 +0100 } 1, 26 -- MIME-Version: 1.0 } 1, 26 -- X-Mailer: Internet Mail Service (5.5.2653.19) } 1, 26 -- Content-Type: text/plain } ==============================End of - } Headers==============================
==============================Original Headers============================== 15, 23 -- From Elliott-at-arizona.edu Thu Mar 29 10:36:58 2007 15, 23 -- Received: from smtpgate.email.arizona.edu (deagol.email.Arizona.EDU [128.196.133.142]) 15, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2TFavjp015875 15, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 29 Mar 2007 10:36:58 -0500 15, 23 -- Received: from localhost (amavis6.email.arizona.edu [10.0.0.209]) 15, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 6C3CD12D4963 15, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 29 Mar 2007 08:36:57 -0700 (MST) 15, 23 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 15, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 61D5912D494C 15, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 29 Mar 2007 08:36:55 -0700 (MST) 15, 23 -- Mime-Version: 1.0 (Apple Message framework v752.2) 15, 23 -- In-Reply-To: {200703290851.l2T8pQrP030541-at-ns.microscopy.com} 15, 23 -- References: {200703290851.l2T8pQrP030541-at-ns.microscopy.com} 15, 23 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 15, 23 -- Message-Id: {94A22B99-0E08-4971-9101-243F0D205989-at-arizona.edu} 15, 23 -- From: David Elliott {Elliott-at-arizona.edu} 15, 23 -- Subject: Re: [Microscopy] TEM help with pre-embedding cells in agar 15, 23 -- Date: Thu, 29 Mar 2007 08:36:54 -0700 15, 23 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 15, 23 -- X-Mailer: Apple Mail (2.752.2) 15, 23 -- X-Virus-Scanned: amavisd-new at email.arizona.edu 15, 23 -- Content-Transfer-Encoding: 8bit 15, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2TFavjp015875 ==============================End of - Headers==============================
Carol - Yet another protocol for agarose embedding is this one, that I got from the University of Bristol Veterinary Pathology website (http://www.bristol.ac.uk/vetpath/cpl/emtechs.htm):
Prepare a 1.5% Solution of Agarose (Sigma Type VII is what I use) in distilled water by bringing to the boil while stirring. Spin samples at 5,000 rpm for 5 minutes. Decant supernatant from sample tubes and take them and the agar solution to the centrifuge. When the agar has cooled to ~60°C, quickly fill each tube with it, resuspend the samples (vortex briefly) and spin them at full speed for 30 seconds to 1 minute (I do a minute at 13,000 rpm). Do a max of 4 at a time or the agar will set before the sample can be spun down to the bottom of the tube. Cool the tubes by putting in a fridge (or you can use a beaker of ice water).
I remove the agar plug by cutting the Eppendorf tube side with a razor blade and pulling out the agar. However, this is not the safest procedure, and I like Debbie Sherman's suggestion about using water or EtOH to remove the plug. I then cut off the end containing the sample, and cut up the sample end into cubes.
Jessica Cervantes Bend Research Inc Bend, Oregon 97701
-----Original Message----- X-from: Carol.Evered-at-warwick.ac.uk [mailto:Carol.Evered-at-warwick.ac.uk] Sent: Thursday, March 29, 2007 1:55 AM To: Cervantes, Jessica
I have been given a protocol for embedding Cyanobacterium synechocystis from liquid culture, after secondary fixation the cells are embedded in 2% agar prior to dehydration and resin embedding. When I have tried this method before the agar has set so rapidly I have had insufficient time to incorporate the cell suspension evenly within the agar. Details of the type of agar, temperatures and method of incorporation are absent so any help on the practicalities of achieving a fairly uniform distribution of cells would be gratefully received. I usually work with the cell suspensions in Eppendorf (micro-centrifuge) tubes. Thanks in advance.
Carol Evered Research Imaging Warwick HRI University of Warwick Wellesbourne Warwick CV35 9EF UK
==============================Original Headers============================== 1, 26 -- From Carol.Evered-at-warwick.ac.uk Thu Mar 29 03:48:04 2007 1, 26 -- Received: from mail-relay-1.csv.warwick.ac.uk (mail-relay-1.warwick.ac.uk [137.205.128.7]) 1, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2T8m3nA025426 1, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 03:48:04 -0500 1, 26 -- Received: from localhost (localhost [127.0.0.1]) 1, 26 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id l2T8m2Xo012504 1, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 09:48:02 +0100 (BST) 1, 26 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 1, 26 -- Received: from mail-relay-1.csv.warwick.ac.uk ([127.0.0.1]) 1, 26 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 1, 26 -- with LMTP id O0MeNzfCO5AF for {Microscopy-at-microscopy.com} ; 1, 26 -- Thu, 29 Mar 2007 09:48:02 +0100 (BST) 1, 26 -- Received: from ntsw02.hri.warwick.ac.uk (f5-snat-dflt [137.205.195.103]) 1, 26 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id l2T8lwbf012424 1, 26 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 Mar 2007 09:47:58 +0100 (BST) 1, 26 -- X-Envelope-From: Carol.Evered-at-hri.ac.uk 1, 26 -- Received: by ntsw02.hri.warwick.ac.uk with Internet Mail Service (5.5.2653.19) 1, 26 -- id {G8TFL3V9} ; Thu, 29 Mar 2007 09:52:38 +0100 1, 26 -- Message-ID: {AE626396FFEDD81199A7000E7FACF9E617E224-at-ntsw14.hri.warwick.ac.uk} 1, 26 -- From: "Evered, Carol" {Carol.Evered-at-warwick.ac.uk} 1, 26 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-microscopy.com} 1, 26 -- Subject: TEM help with pre-embedding cells in agar 1, 26 -- Date: Thu, 29 Mar 2007 09:48:27 +0100 1, 26 -- MIME-Version: 1.0 1, 26 -- X-Mailer: Internet Mail Service (5.5.2653.19) 1, 26 -- Content-Type: text/plain ==============================End of - Headers==============================
==============================Original Headers============================== 8, 16 -- From cervantes-at-bendres.com Thu Mar 29 10:49:49 2007 8, 16 -- Received: from BRIEX04A.bri.local (bc161112.bendcable.com [216.228.161.112]) 8, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2TFnmLV027435 8, 16 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 10:49:49 -0500 8, 16 -- MIME-Version: 1.0 8, 16 -- Content-Type: text/plain; 8, 16 -- charset="iso-8859-1" 8, 16 -- Subject: RE: [Microscopy] TEM help with pre-embedding cells in agar 8, 16 -- Date: Thu, 29 Mar 2007 08:49:48 -0700 8, 16 -- Message-ID: {8943D65F9AD70E4488AD6DE09F15088768C40E-at-BRIEX04A} 8, 16 -- In-Reply-To: {200703290854.l2T8ssmx003087-at-ns.microscopy.com} 8, 16 -- References: {200703290854.l2T8ssmx003087-at-ns.microscopy.com} 8, 16 -- From: "Cervantes, Jessica" {cervantes-at-bendres.com} 8, 16 -- To: {Microscopy-at-microscopy.com} 8, 16 -- Content-Transfer-Encoding: 8bit 8, 16 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2TFnmLV027435 ==============================End of - Headers==============================
We have also done this technique several times with great success using both low melting point agarose and sometimes using 12% gelatin. The concern with using agarose is the potential to introduce bubbles which sometimes don't move during centrifugation. Using gelatin is sometimes easier because of the lower viscosity, even at 12% and as long as you keep the gelatin below 30 C it will stay solid. We buy our gelatin from the supermarket, yes, the same stuff used for cooking and making jellies (we use Knox gelatin). This evolved from using gelatin for the Tokuyasu technique.
Good luck,
Garnet
-- Garnet Martens
Research Manager BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
phone 604-822-3354
==============================Original Headers============================== 7, 25 -- From gmartens-at-interchange.ubc.ca Thu Mar 29 10:57:55 2007 7, 25 -- Received: from mta2.mail-relay.ubc.ca (mta2.mail-relay.ubc.ca [137.82.45.4]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2TFvtT5006485 7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 10:57:55 -0500 7, 25 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 7, 25 -- by mta2.mail-relay.ubc.ca (8.12.11.20060308/8.12.11) with ESMTP id l2TFvrNH005899 7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 08:57:53 -0700 (PDT) 7, 25 -- (envelope-from gmartens-at-interchange.ubc.ca) 7, 25 -- Received: from [137.82.85.216] (0511.emlab.ubc.ca [137.82.85.216]) 7, 25 -- by smtp.interchange.ubc.ca 7, 25 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 7, 25 -- with ESMTPA id {0JFO00EB69OG68-at-smtp.interchange.ubc.ca} for 7, 25 -- microscopy-at-microscopy.com; Thu, 29 Mar 2007 08:57:53 -0700 (PDT) 7, 25 -- Date: Thu, 29 Mar 2007 08:57:51 -0700 7, 25 -- From: Garnet Martens {gmartens-at-interchange.ubc.ca} 7, 25 -- Subject: Re: TEM help with pre-embedding cells in agar 7, 25 -- To: microscopy-at-microscopy.com 7, 25 -- Message-id: {a06240802c2318f40dbac-at-[137.82.85.216]} 7, 25 -- MIME-version: 1.0 7, 25 -- Content-type: text/plain; format=flowed; charset=us-ascii 7, 25 -- X-UBC-Scanned: Sophos PureMessage 4.7.1.128075, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.3.29.75734 7, 25 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 7, 25 -- X-PerlMx-Spam: Probability=7%, Report=__C230066_P5 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0 7, 25 -- X-Spam-Level: 7, 25 -- X-Spam-Flag: No ==============================End of - Headers==============================
A digital TEM camera essentially consists of a screen that converts electrons to photons, and a camera system that takes images of that screen. There are several resolution limiting elements in this chain. The fist is the TEM itself. Let's say it has a resolution of 0.2 nm. Then there is the phosophor screen (in most cases) that converts the electrons to photons. Each electron scatters in the screen and creates multiple photons. The efect is that a single electron creates a light spot of a size that depends on the thickness and composition of the film, the accelerating voltage, etc. For simplicity, let's say that this light spot is 10 microns. Finally, you have the camera system that records these light spots. It has a resolution itself, which depends on pixel size and optics. All of this is governed by the Nyquist or Shannon theorem which set theoretical limits to the resolution.
The critical part in this chain is the phosphor. If you have a 10 micron resolution, and you are working at, let's say, 1000x, a 10 micron spot on the phosphor will show a roughly 10 nm spot of the sample. In other words, in this situation your resolution will be limited to 10 nm, no matter how good the resolution of your TEM is. Only if you work above 50,000X does the resolution of the microscope start playing a role.
So, if you have a camera that is designed for highest resolution (meaning that it can record all the information that comes from the phosphor at the Nyqust limit), and you increase the number of pixels, you will not gain an increase in resolution, but in field of view. If, on the other hand, the camera was designed for maximum field of view, and you keep the field of view, an increase in the number of pixels might result in an increase in resolution, if the smaller camera did not meet the Nyquist limit. This is the case as long as the resolution limiting factor is the phosphor. If you go way up in magnification, the resolution limiting factor might be the TEM, and in those cases it is possible that adding pixels only adds empty resolution.
As far as your "1000" pixel question is concerned, the answer is again 2-fold. If you are in a magnification range where the phosphor is the limiting factor, an increase in magnification will also increase the resolution. If you are in a range where the TEM limits the resolution, you will only get a larger image of the same blurry blob, and sacrifice field of view. In that case it would be better to take the "500 pixel" image, and simply have a computer interpolate the data to increase the number of pixels and then use that as a source of the analysis. (you would have to document that, though, to avoid charges of "unethical image processing")
Michael Bode, Ph.D.
General Manager
OLYMPUS SOFT IMAGING SOLUTIONS 12596 West Bayaud Ave #300 Lakewood, CO 80228 USA Tel.: +1 (303) 234-9270 Fax.: +1 (303) 234-9271 E-mail: Mike.Bode-at-olympus-sis.com www.olympus-sis.com
-----Original Message----- X-from: frank.karl-at-degussa.com [mailto:frank.karl-at-degussa.com] Sent: Thursday, March 29, 2007 06:49 To: Mike Bode
Let me float an question to the list. What is the effect of changing a one meg camera to a three meg camera on a TEM?
I'm asked some fundamental questions, and I can't seem to answer them to this person's satisfaction, which implies I don't have a good grasp of the question or answers.
My immediate response is you would increase the resolution. I can envision the image size on the monitor changing, but if the resolution of the screen is lower than the captured image and if the computer/imaging software wants to display all the image captured, will not any feature at a specific magnification have the resolution of the monitor? It seems the same is true for the printer. I can't simply expand the size of the paper at will, so the software will either reduce the printed image magnification or print just a smaller section of the total image. Again, since the printer has a fixed resolution will not the printed image resolution will be limited by the printer's (This sounds like a Hi-Fi discussion from the early 60's... just change the words...) upper limit?
So why capture high resolution images? My response is it allows you to post process and expand the image to examine one feature and have sufficient "stored" resolution to display the image without empty magnification. This also implies (to me at least) if I want to measure from point A to point B, the more camera pixels I have the better I can resolve where point A starts and point B ends which should allow me to have better confidence in my measurements. I believe that imaging software works on the image in memory and not the image displayed on the screen so size of the print or screen has little to do with data obtained. It's more a function of the size of the captured image?
Lastly.....
If I feel the need to have at least 1000 pixels in an image feature, and due to my camera I can only capture 500 at a magnification X, is there any reason not to simply increase the magnification so I have a larger feature which now occupies more pixels. I realize I haven't increased the resolution, but if my software need 999 pixels to "recognize" a feature haven't I met this requirement?
Thank in advance! Frank (I miss film) Karl
==============================Original Headers============================== 10, 17 -- From frank.karl-at-degussa.com Thu Mar 29 07:42:34 2007 10, 17 -- Received: from framailout1.rz.itson.com (mailout2.degussa.com [149.216.91.173]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2TCgXHD021519 10, 17 -- for {microscopy-at-msa.microscopy.com} ; Thu, 29 Mar 2007 07:42:33 -0500 10, 17 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [10.88.88.98]) 10, 17 -- by framailout1.rz.itson.com (8.13.4/8.13.4/Debian-3sarge3) with ESMTP id l2TCg2Bb031526 10, 17 -- for {microscopy-at-msa.microscopy.com} ; Thu, 29 Mar 2007 14:42:29 +0200 10, 17 -- Subject: Digital cameras and the TEM 10, 17 -- To: microscopy-at-msa.microscopy.com 10, 17 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 10, 17 -- Message-ID: {OF3BDC677E.DA558E0C-ON862572AD.003F9EC7-852572AD.0045C6B9-at-degussa.com} 10, 17 -- From: frank.karl-at-degussa.com 10, 17 -- Date: Thu, 29 Mar 2007 08:42:21 -0400 10, 17 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 10, 17 -- 03/29/2007 07:42:30 AM 10, 17 -- MIME-Version: 1.0 10, 17 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
==============================Original Headers============================== 28, 26 -- From Mike.Bode-at-olympus-sis.com Thu Mar 29 11:03:02 2007 28, 26 -- Received: from mail.soft-imaging.de (ns.soft-imaging.de [62.180.61.130]) 28, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2TG31CK016201 28, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 11:03:02 -0500 28, 26 -- Received: from muenster.olympus-sis.com (muenster.olympus-sis.com [10.26.20.9]) 28, 26 -- by mail.soft-imaging.de (8.13.3/8.13.3/SuSE Linux 0.7) with ESMTP id l2TGBcMG016412; 28, 26 -- Thu, 29 Mar 2007 18:11:40 +0200 28, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 28, 26 -- Content-class: urn:content-classes:message 28, 26 -- MIME-Version: 1.0 28, 26 -- Content-Type: text/plain; 28, 26 -- charset="us-ascii" 28, 26 -- Subject: RE: [Microscopy] Digital cameras and the TEM 28, 26 -- Date: Thu, 29 Mar 2007 18:00:55 +0200 28, 26 -- Message-ID: {78B53BA1C5A2D9449EDA30A98800EC9486328F-at-ms-s-gws.soft-imaging.net} 28, 26 -- In-Reply-To: {200703291248.l2TCmWTd029876-at-ns.microscopy.com} 28, 26 -- X-MS-Has-Attach: 28, 26 -- X-MS-TNEF-Correlator: 28, 26 -- Thread-Topic: [Microscopy] Digital cameras and the TEM 28, 26 -- Thread-Index: AcdyAJFp1oFOcuyRRNCmqSAs27DbWAAFStOQ 28, 26 -- References: {200703291248.l2TCmWTd029876-at-ns.microscopy.com} 28, 26 -- From: "Mike Bode" {Mike.Bode-at-olympus-sis.com} 28, 26 -- To: {Microscopy-at-microscopy.com} 28, 26 -- Cc: {frank.karl-at-degussa.com} 28, 26 -- Content-Transfer-Encoding: 8bit 28, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2TG31CK016201 ==============================End of - Headers==============================
Gelatin or agarose can be used to support cell suspensions for subsequent sectioning. One advantage of gelatin over agarose is that if the gel sets before the cells have been pelleted down, the gelatin can be easily liquefied by warming to 37 degrees. Agarose needs a little more heating to liquefy.
One important point to remember is that if the cells have been fixed in aldehyde, residual aldehyde has to be either removed or quenched or it will cross-link gelatin or agarose before you are ready to let it gel. Wash the cells in a low concentration of ammonium chloride, lycine or glycine before embedding in the gel.
Regards,
Paul Webster.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
We have also done this technique several times with great success using both low melting point agarose and sometimes using 12% gelatin. The concern with using agarose is the potential to introduce bubbles which sometimes don't move during centrifugation. Using gelatin is sometimes easier because of the lower viscosity, even at 12% and as long as you keep the gelatin below 30 C it will stay solid. We buy our gelatin from the supermarket, yes, the same stuff used for cooking and making jellies (we use Knox gelatin). This evolved from using gelatin for the Tokuyasu technique.
Good luck,
Garnet
==============================Original Headers============================== 13, 18 -- From PWebster-at-hei.org Thu Mar 29 11:50:58 2007 13, 18 -- Received: from hi0sml1.hei.org (House-Ear-Institute-1114861.cust-rtr.pacbell.net [71.136.31.242]) 13, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2TGovNA030393 13, 18 -- for {microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 11:50:57 -0500 13, 18 -- Received: from 10.10.42.123 ([10.10.42.123]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 13, 18 -- Thu, 29 Mar 2007 16:50:56 +0000 13, 18 -- User-Agent: Microsoft-Entourage/11.3.3.061214 13, 18 -- Date: Thu, 29 Mar 2007 09:50:55 -0700 13, 18 -- Subject: TEM help with pre-embedding cells in agar 13, 18 -- From: "Webster, Paul" {PWebster-at-hei.org} 13, 18 -- To: {microscopy-at-microscopy.com} 13, 18 -- Message-ID: {C2313C7F.1057B%PWebster-at-hei.org} 13, 18 -- Thread-Topic: TEM help with pre-embedding cells in agar 13, 18 -- Thread-Index: AcdyImrSqWeV2N4VEduHsgANk7Zh7g== 13, 18 -- Mime-version: 1.0 13, 18 -- Content-type: text/plain; 13, 18 -- charset="US-ASCII" 13, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
I have read with interest this thread on embedding cells in agar. The procedure I have used successfully for years is quite close to that described by Debby Sherman. As she and others have pointed out, its necessary to keep the melted agar, fixed and washed cell pellets, pipets, tubes, etc warm to prevent premature freezing of the agar, so it goes into the microfuge at about 40 C. I then spin down for 10 minutes at 14,000 rpm, a bit more than others have recommended, but I want to be sure that I get those puppies down!
I'm not sure why you want to evenly disperse the cells into the agar. Usually a tight, enrobed pellet is desired so that when you view sections you will see lots of cells fairly close together. But it is necessary to gently mix the cells into the agar just a little to effectively enrobe them but without diluting the pellet too much for the above reason.
I mix my low melting point agarose (Sigma, # A9414) to 2% w/v, and keep my water bath for keeping the melted agar and cell pellets warm in 1.5 ml Eppendorf tubes at about 42C. Between use, I store the dissolved agarose stock in the freezer.
The only other thing I would add to this discussion is to point out a paper by Jaqueline Wood and Karen Klomparens in which agarose, agar and gelatin were compared as encapsulating media for bacteria, yeast and mitochondria. They conclude that agarose has advantages over the other two, mainly that it contributes the least background density in the TEM image. As a result of reading this paper, I switched from agar to agarose. The reference is:
Wood, Jacqueline I. and Klomparens, Karen L. 1993. Characterization of agarose as an encapsulation medium for particulate specimens for transmission electron microscopy. Microscopy Research and Technique 25:267-275.
Good luck!
Gib --------- Gilbert (Gib) Ahlstrand, Electron Microscopist, Imaging Center, University of Minnesota 123 Snyder Hall St. Paul, MN 55108 http://www.cbs.umn.edu/ic ----------
Carol.Evered-at-warwick.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I have been given a protocol for embedding Cyanobacterium synechocystis from } liquid culture, after secondary fixation the cells are embedded in 2% agar } prior to dehydration and resin embedding. } When I have tried this method before the agar has set so rapidly I have had } insufficient time to incorporate the cell suspension evenly within the agar. } Details of the type of agar, temperatures and method of incorporation are } absent so any help on the practicalities of achieving a fairly uniform } distribution of cells would be gratefully received. I usually work with the } cell suspensions in Eppendorf (micro-centrifuge) tubes. } Thanks in advance. } } Carol Evered } Research Imaging } Warwick HRI } University of Warwick } Wellesbourne } Warwick } CV35 9EF } UK }
==============================Original Headers============================== 9, 21 -- From ahlst007-at-umn.edu Thu Mar 29 11:59:29 2007 9, 21 -- Received: from mta-a2.tc.umn.edu (mta-a2.tc.umn.edu [134.84.119.206]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2TGxSsV009408 9, 21 -- for {Microscopy-at-Microscopy.com} ; Thu, 29 Mar 2007 11:59:28 -0500 9, 21 -- Received: from [160.94.39.28] (x160-94-39-28.cbs.umn.edu [160.94.39.28]) 9, 21 -- by mta-a2.tc.umn.edu (UMN smtpd) with ESMTP 9, 21 -- for {Microscopy-at-Microscopy.com} ; Thu, 29 Mar 2007 11:59:28 -0500 (CDT) 9, 21 -- X-Umn-Remote-Mta: [N] x160-94-39-28.cbs.umn.edu [160.94.39.28] #+LO+TS+AU+HN 9, 21 -- Message-ID: {460BEFF4.2090701-at-umn.edu} 9, 21 -- Date: Thu, 29 Mar 2007 11:57:24 -0500 9, 21 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 9, 21 -- Reply-To: ahlst007-at-umn.edu 9, 21 -- Organization: Imaging Center UM 9, 21 -- User-Agent: Thunderbird 1.5.0.10 (Macintosh/20070221) 9, 21 -- MIME-Version: 1.0 9, 21 -- To: Microscopy-at-Microscopy.com 9, 21 -- Subject: Re: [Microscopy] TEM help with pre-embedding cells in agar 9, 21 -- References: {200703290851.l2T8pkSo031139-at-ns.microscopy.com} 9, 21 -- In-Reply-To: {200703290851.l2T8pkSo031139-at-ns.microscopy.com} 9, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I would just like to add that we use 2% "ultra-low gelling agarose" Sigma Type IX. Mix in 2x strength buffer and use vol equal to cell suspension. This agarose melts at ~50C but only gels at 8C-17C, allowing you to work with tissues at room temp and then gel when you are ready by placing on ice or in refrigerator a few minutes. For very sensitive materials, working at ~25C may be an advantage. In other respects it is handled just as detailed in all the other replies. I use a piece of sheet Teflon from Small Parts Inc. to work on and the agarose drops easily float off when set.
Dale
Carol.Evered-at-warwick.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I have been given a protocol for embedding Cyanobacterium synechocystis from } liquid culture, after secondary fixation the cells are embedded in 2% agar } prior to dehydration and resin embedding. } When I have tried this method before the agar has set so rapidly I have had } insufficient time to incorporate the cell suspension evenly within the agar. } Details of the type of agar, temperatures and method of incorporation are } absent so any help on the practicalities of achieving a fairly uniform } distribution of cells would be gratefully received. I usually work with the } cell suspensions in Eppendorf (micro-centrifuge) tubes. } Thanks in advance. } } Carol Evered } Research Imaging } Warwick HRI } University of Warwick } Wellesbourne } Warwick } CV35 9EF } UK } } ==============================Original Headers============================== } 1, 26 -- From Carol.Evered-at-warwick.ac.uk Thu Mar 29 03:48:04 2007 } 1, 26 -- Received: from mail-relay-1.csv.warwick.ac.uk (mail-relay-1.warwick.ac.uk [137.205.128.7]) } 1, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2T8m3nA025426 } 1, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 03:48:04 -0500 } 1, 26 -- Received: from localhost (localhost [127.0.0.1]) } 1, 26 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id l2T8m2Xo012504 } 1, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 09:48:02 +0100 (BST) } 1, 26 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk } 1, 26 -- Received: from mail-relay-1.csv.warwick.ac.uk ([127.0.0.1]) } 1, 26 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) } 1, 26 -- with LMTP id O0MeNzfCO5AF for {Microscopy-at-microscopy.com} ; } 1, 26 -- Thu, 29 Mar 2007 09:48:02 +0100 (BST) } 1, 26 -- Received: from ntsw02.hri.warwick.ac.uk (f5-snat-dflt [137.205.195.103]) } 1, 26 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id l2T8lwbf012424 } 1, 26 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 Mar 2007 09:47:58 +0100 (BST) } 1, 26 -- X-Envelope-From: Carol.Evered-at-hri.ac.uk } 1, 26 -- Received: by ntsw02.hri.warwick.ac.uk with Internet Mail Service (5.5.2653.19) } 1, 26 -- id {G8TFL3V9} ; Thu, 29 Mar 2007 09:52:38 +0100 } 1, 26 -- Message-ID: {AE626396FFEDD81199A7000E7FACF9E617E224-at-ntsw14.hri.warwick.ac.uk} } 1, 26 -- From: "Evered, Carol" {Carol.Evered-at-warwick.ac.uk} } 1, 26 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-microscopy.com} } 1, 26 -- Subject: TEM help with pre-embedding cells in agar } 1, 26 -- Date: Thu, 29 Mar 2007 09:48:27 +0100 } 1, 26 -- MIME-Version: 1.0 } 1, 26 -- X-Mailer: Internet Mail Service (5.5.2653.19) } 1, 26 -- Content-Type: text/plain } ==============================End of - Headers==============================
==============================Original Headers============================== 4, 21 -- From dac-at-research.umass.edu Thu Mar 29 13:08:34 2007 4, 21 -- Received: from race6.oit.umass.edu (race6.oit.umass.edu [128.119.101.44]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2TI8XmS022296 4, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 13:08:33 -0500 4, 21 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 4, 21 -- (authenticated bits=0) 4, 21 -- by race6.oit.umass.edu (8.13.7/8.13.7) with ESMTP id l2TI8Wc0019659 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 4, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 14:08:33 -0400 4, 21 -- Message-ID: {460C0ECC.7060702-at-research.umass.edu} 4, 21 -- Date: Thu, 29 Mar 2007 14:09:00 -0500 4, 21 -- From: Dale Callaham {dac-at-research.umass.edu} 4, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.2) Gecko/20070222 SeaMonkey/1.1.1 4, 21 -- MIME-Version: 1.0 4, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 21 -- Subject: Re: [Microscopy] TEM help with pre-embedding cells in agar 4, 21 -- References: {200703290854.l2T8srXt003057-at-ns.microscopy.com} 4, 21 -- In-Reply-To: {200703290854.l2T8srXt003057-at-ns.microscopy.com} 4, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 21 -- Content-Transfer-Encoding: 7bit 4, 21 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Dear colleagues We would like to bring to your attention the Symposium on Scanning Probe Microscopy applications for characterization of nanoscale phenomena in functional nanomaterials, to be held at the Materials Research Society 2007 Fall meeting in Boston, MA. The full text for call for papers is available at http://www.mrs.org/s_mrs/sec.asp?CID=8005&DID=192095 and is also listed below. The deadline for abstract submission is June 20. Looking forward to seeing you in Boston! On behalf of the organizers Sergei V. Kalinin
MRS 2007 Fall meeting - Symposium B
Nanoscale Phenomena in Functional Materials by Scanning Probe Microscopy
The last decade has witnessed spectacular progress in the development and applications of scanning probe microscopy (SPM)-based nanoscale imaging techniques. The combination of high spatial resolution and sensitivity to local electronic, optical, and mechanical properties places these techniques among the most versatile tools for nanoscience, biology, physics, and materials science. Atomic and electronic structure of surfaces, vibrational excitations, energy flow, and local materials properties on the molecular level has become accessible with the advent of high-resolution SPMs. Electrostatic SPMs are being established as powerful techniques for spatially resolved studies of electronic transport on the nanometer level at electroactive interfaces and in molecular electronic devices such as carbon nanotubes. Dynamic SPM modes and scanning indentation techniques allow mechanical compliance and surface energy to be investigated at the nanoscale with applications to the emerging fields of nanotribology, nanofluidics, nanocomposites, and NEMS. Novel optical imaging modes, such as tip-enhanced spectroscopy, solid immersion microscopy, and apertureless scanning optical microscopy, have joined the now-established near-field scanning optical microscopy (NSOM), bringing the resolution of optical spectroscopy into the nanoscale regime and complementing local electronic measurements of materials with the STM family of instruments. These new measurement techniques were necessitated by the growing need for materials characterization on the nanoscale and have in turn led to the discovery of new nanoscale phenomena. Finally, the SPM has been used to manipulate and fabricate materials at the nanoscale.
It is the goal of this symposium to provide a multidisciplinary forum for scanning-probe-based materials and nanoscience in order to demonstrate the latest achievements in technique developments and materials applications that have led to scientific discoveries. The symposium will include two types of sessions: One will be dedicated to the recent advances in technique development of interest to the materials community and will bring together specialists in practical and theoretical aspects of SPM imaging. The second will focus on specific materials-related phenomena, including nanotubes and nanowires, quantum dots, surfaces, interfaces, and biological systems studied by local probe techniques.
The topics of the symposium will include, but not be limited to:
- Imaging, manipulation, and energy transfer on the atomic and molecular level by atomic resolution NC-AFM and STM - Local optical and electronic properties and excitations, e.g., plasmons measured with SPMs - Defects, impurities, dopants, and transport in semiconductor nanostructures, nanotubes, and nanowires - Mechanics and electromechanics on the nanoscale by SPM and nanoindentation - Mechanical and voltage nanolithography and surface modification - Energy flows and dissipation in materials, devices, and nanostructures
- Transport in single-molecule devices and carbon nanotubes - Imaging and characterization of ferroelectric materials - Electronic properties of semiconductor heterostructures - Imaging and characterization of biological systems - Dynamics and imaging of polymers and soft materials
Invited speakers include: Robert Carpick (Univ. of Pennsylvania), Levent Degertekin (Georgia Inst. of Technology), Dennis Discher (Univ. of Pennsylvania), Ricardo Garcia (Univ. Madrid, Spain), Franz Giessibl (Univ. Augsburg, Germany), Venkat Gopalan (Pennsylvania State Univ.), Jan Hoh (Johns Hopkins Univ.), Ernesto Joselevich (Weizmann Inst. of Science, Israel), Maki Kawai (RIKEN, Japan), L. Kuipers (FOM, The Netherlands), Alexander Malkin (Lawrence Livermore National Lab), Lukas Novotny (Univ. of Rochester), E. Ward Plummer (Univ. of Tennessee/Oak Ridge National Lab), V. Sandoghdar (ETH Zurich, Switzerland), M. Tomitori (JAIST, Japan), and S. Wilks (Swansea Univ., United Kingdom).
Symposium Organizers
Dawn Bonnell University of Pennsylvania, Nano/Bio Interface Center, 3231 Walnut St., Philadelphia, PA 19104 Tel 215-898-6231, Fax 215-746-3204, bonnell-at-lrsm.upenn.edu
Sergei V. Kalinin Oak Ridge National Laboratory, Materials Sciences and Technology Division and Center for Nanophase Materials Sciences, 1 Bethel Valley Rd., Oak Ridge, TN 37831 Tel 865-241-0236, Fax 865-574-4143, sergei2-at-ornl.gov
Sidney R. Cohen Weizmann Institute of Science, Surface Analysis Laboratory, Rehovot 76100 Israel Tel 972-8-934-2703/3422, Fax 972-8-934-4137, sidney.cohen-at-weizmann.ac.il
Richard E. Palmer University of Birmingham, School of Physics and Astronomy, Nanoscale Physics Research Laboratory, Birmingham B15 2TT, United Kingdom Tel 44-121-414-4653, Fax 44-121-414-7327, r.e.palmer-at-bham.ac.uk
-- Sergei V. Kalinin, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Bldg. 3025, MS6030, Oak Ridge, TN 37831 Phone: (865) 241-0236 FAX: (865) 574-4143 URL: sergei2.kalininweb.com New: http://nanotransport.ornl.gov
==============================Original Headers============================== 18, 33 -- From sergei2-at-ornl.gov Thu Mar 29 14:14:03 2007 18, 33 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119]) 18, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2TJE2V2003019 18, 33 -- for {microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 14:14:02 -0500 18, 33 -- Received: from emroute1.ornl.gov ([127.0.0.1]) 18, 33 -- by emroute1.ornl.gov (PMDF V6.3-x3 #31246) 18, 33 -- with ESMTP id {0JFO00B0HIRD9N-at-emroute1.ornl.gov} for 18, 33 -- microscopy-at-microscopy.com; Thu, 29 Mar 2007 15:14:02 -0400 (EDT) 18, 33 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov 18, 33 -- (PMDF V6.3-x3 #31246) id {0JFO00B01IRCFE-at-emroute1.ornl.gov} for 18, 33 -- microscopy-at-microscopy.com; Thu, 29 Mar 2007 15:14:00 -0400 (EDT) 18, 33 -- Received: from ORNLEXCHANGE.ornl.gov (ornlexchange2.ornl.gov [160.91.1.22]) 18, 33 -- by emroute1.ornl.gov (PMDF V6.3-x3 #31246) 18, 33 -- with ESMTP id {0JFO0056ZIRC30-at-emroute1.ornl.gov} for 18, 33 -- microscopy-at-microscopy.com; Thu, 29 Mar 2007 15:14:00 -0400 (EDT) 18, 33 -- Date: Thu, 29 Mar 2007 15:08:26 -0400 18, 33 -- From: "Kalinin, Sergei V." {sergei2-at-ornl.gov} 18, 33 -- Subject: MRS Fall 2007 - Symposium on Scanning Probe Microscopy 18, 33 -- To: microscopy-at-microscopy.com 18, 33 -- Message-id: {3E4D6911DB2CA94DA31AC5E1CB522DBB01712421-at-ORNLEXCHANGE.ornl.gov} 18, 33 -- MIME-version: 1.0 18, 33 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 18, 33 -- Content-type: text/plain; charset=us-ascii 18, 33 -- Importance: high 18, 33 -- Priority: Urgent 18, 33 -- X-Priority: 1 18, 33 -- Thread-Topic: MRS Fall 2007 - Symposium on Scanning Probe Microscopy 18, 33 -- Thread-Index: AcdyNaFhb/oh+faKRRKMQ4hUuzAJgw== 18, 33 -- Content-class: urn:content-classes:message 18, 33 -- X-MS-Has-Attach: 18, 33 -- X-MS-TNEF-Correlator: 18, 33 -- Content-Transfer-Encoding: 8bit 18, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2TJE2V2003019 ==============================End of - Headers==============================
As I have mentioned in an earlier post an alternative that is to encapsulate in alginate, we have used this to encapsulate both individual cells and tissues. The advantage is that you work at ambient temperature (no heating), disadvantage that you introduce calcium ions into the system which you may not want to do). One method we used was to use a 2% solution of sodium alginate and solidify by dropping into or flooding with 50 mM Calcium Chloride.
I have used this with plant cell suspensions in the past after the primary fixation.
Ian
Ian Hallett Sensory and Consumer Science - Microscopy HortResearch, Mt Albert Research Centre Private Bag 92 169, Auckland Mail Centre Auckland 1142, New Zealand +64-9-815 4200 ext 7002
-----Original Message----- X-from: Carol.Evered-at-warwick.ac.uk [mailto:Carol.Evered-at-warwick.ac.uk] Sent: Thursday, 29 March 2007 8:51 p.m. To: Ian Hallett
I have been given a protocol for embedding Cyanobacterium synechocystis from liquid culture, after secondary fixation the cells are embedded in 2% agar prior to dehydration and resin embedding. When I have tried this method before the agar has set so rapidly I have had insufficient time to incorporate the cell suspension evenly within the agar. Details of the type of agar, temperatures and method of incorporation are absent so any help on the practicalities of achieving a fairly uniform distribution of cells would be gratefully received. I usually work with the cell suspensions in Eppendorf (micro-centrifuge) tubes. Thanks in advance.
Carol Evered Research Imaging Warwick HRI University of Warwick Wellesbourne Warwick CV35 9EF UK
==============================Original Headers============================== 1, 26 -- From Carol.Evered-at-warwick.ac.uk Thu Mar 29 03:48:04 2007 1, 26 -- Received: from mail-relay-1.csv.warwick.ac.uk (mail-relay-1.warwick.ac.uk [137.205.128.7]) 1, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2T8m3nA025426 1, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 03:48:04 -0500 1, 26 -- Received: from localhost (localhost [127.0.0.1]) 1, 26 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id l2T8m2Xo012504 1, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 09:48:02 +0100 (BST) 1, 26 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 1, 26 -- Received: from mail-relay-1.csv.warwick.ac.uk ([127.0.0.1]) 1, 26 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 1, 26 -- with LMTP id O0MeNzfCO5AF for {Microscopy-at-microscopy.com} ; 1, 26 -- Thu, 29 Mar 2007 09:48:02 +0100 (BST) 1, 26 -- Received: from ntsw02.hri.warwick.ac.uk (f5-snat-dflt [137.205.195.103]) 1, 26 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id l2T8lwbf012424 1, 26 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 Mar 2007 09:47:58 +0100 (BST) 1, 26 -- X-Envelope-From: Carol.Evered-at-hri.ac.uk 1, 26 -- Received: by ntsw02.hri.warwick.ac.uk with Internet Mail Service (5.5.2653.19) 1, 26 -- id {G8TFL3V9} ; Thu, 29 Mar 2007 09:52:38 +0100 1, 26 -- Message-ID: {AE626396FFEDD81199A7000E7FACF9E617E224-at-ntsw14.hri.warwick.ac.uk} 1, 26 -- From: "Evered, Carol" {Carol.Evered-at-warwick.ac.uk} 1, 26 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-microscopy.com} 1, 26 --
Version 2.0.1 of the edm software has been officially released. The main changes are: 1) Much better (and faster) analysis of images and diffraction patterns 2) Reasonable structure completion code 3) CBED simulation 4) Anonymous CVS, Windows versions 5) All for exactly $0.00
For details see www.numis.northwestern.edu/edm and http://www.numis.northwestern.edu/edm/documentation/edm.htm
==============================Original Headers============================== 2, 26 -- From marksmsa-at-gmail.com Thu Mar 29 18:38:10 2007 2, 26 -- Received: from nf-out-0910.google.com (nf-out-0910.google.com [64.233.182.191]) 2, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2TNcACT031127 2, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 18:38:10 -0500 2, 26 -- Received: by nf-out-0910.google.com with SMTP id a4so450926nfc 2, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 16:38:09 -0700 (PDT) 2, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 2, 26 -- d=gmail.com; s=beta; 2, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 2, 26 -- b=BAb8IZfzXJ93uDhmuIEdZHSyYet5me/6SGx2YAXS9XGMLe2zQVbDj/FaOEXPoe0wb58A/cCO/O96xyWsvXAFnKmRIWLjtl3Xcion8hrulfxiDVrmwp7uEx5I80Ps1Yw44XMG9agrsgsIAmcFcwHXrF+In/kcs+PHpWfzuRzOYSk= 2, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 2, 26 -- d=gmail.com; s=beta; 2, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 2, 26 -- b=c44NJHO89YyQIC1bwxRxf6C8cETbguh0iDiibWOnuVvgGwDBud7l+SZaLEpN9Aorzbp693soH6+jr1r7pXFK8n9Z9hpcxOlvU+BfTlT/Xbf4uMoqw/jTxmb/r8YPRXCKg+B0PD0gTqJuXLg3Js5SsGbQ2gaNSBbeCc+UyFtEFvE= 2, 26 -- Received: by 10.78.149.15 with SMTP id w15mr668423hud.1175211489062; 2, 26 -- Thu, 29 Mar 2007 16:38:09 -0700 (PDT) 2, 26 -- Received: by 10.78.52.13 with HTTP; Thu, 29 Mar 2007 16:38:09 -0700 (PDT) 2, 26 -- Message-ID: {e13ba6260703291638r6e8ddfco32a534571dd7909f-at-mail.gmail.com} 2, 26 -- Date: Thu, 29 Mar 2007 18:38:09 -0500 2, 26 -- From: "L Marks" {marksmsa-at-gmail.com} 2, 26 -- To: Microscopy-at-microscopy.com 2, 26 -- Subject: EDM 2.0.1 2, 26 -- MIME-Version: 1.0 2, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 2, 26 -- Content-Transfer-Encoding: 7bit 2, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jmastrangelo-at-ulbi.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jmastrangelo-at-ulbi.com Name: Joe Mastrangelo
Organization: Ultralife Batteries
Title-Subject: [Filtered] Old Microscopes
Question: We have a couple of older looking microscopes gathering dust in the back of our lab that look like they may have been very nice instruments at one time. Unfortunately, I have no idea how old they are or whether they are fully operational. I have scoured the lab for the manuals, but haven't had any luck finding any useful information on them.
The microscopes are an Olympus BH-2 and a Nikon EPIPHOT.
My hope is to clean them up and get them fully operational again, but I do not have extensive experience working with these older optical microscopes. My questions to the list are as follows:
1) Does anyone have information or manuals on either of these scopes that they would be willing to share?
2) Is it possible to still obtain parts or service for these scopes, if needed?
3) The Olympus scope looks like it has a camera mounted on the top of the optical column. If I can get this scope working, will I be able to find film that still works in this camera?
Thanks so much in advance for your help. This list is a wonderful resource.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/ --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rra-at-stowers-institute.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rra-at-stowers-institute.org Name: Rhonda Allen
Organization: Stowers Institute
Title-Subject: [Filtered] Leica EM-Stain
Question: Hello, I am interested in purchasing the Leica EM-Stain. Does anyone have any comments, positive and negative, that I should take into consideration before making such an investment? We will be using it on formvar coated slot grids. Thanks in advance, Rhonda Allen BA HT(ASCP)HTL, QIHC Stowers Institute for Medical Research Kansas City, Missouri 816-926-4346
Anyone using this system? I'm using mine with a Jeol 6100 SEM, and it works but there are image defects--different ones for each mode:
In raw mode, the picture is noisy.
In average mode, the noise is much better, but it has a bug in which pure white (value 255) is instead represented as pure black (value 0). This is an obvious bug caused by numerical "wrap around."
In integrate mode, the picture suffers from random horizontal skewing, which both other modes have also, but it's worse in Integrate mode.
Has anyone else encounters such problems with this system and developed a work around?
Does anyone have a software manual? Does it say more than the software's Help?
I do have the installation manual. And I know that the Olympus Adda III would make a great replacement.
Thanks!
-- Bernard R. Cuzzillo, Ph.D., P.E. President, Mechanical Engineer, and Fire Scientist Berkeley Research Company (BRC) 600 Addison Street Berkeley, CA 94710-1920 USA
bernard-at-berkeleyrc.com
Cell phone: 510.821.2499
==============================Original Headers============================== 11, 28 -- From biggerdadda-at-gmail.com Thu Mar 29 21:04:44 2007 11, 28 -- Received: from an-out-0708.google.com (an-out-0708.google.com [209.85.132.247]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2U24hqi003588 11, 28 -- for {microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 21:04:43 -0500 11, 28 -- Received: by an-out-0708.google.com with SMTP id b20so364951ana 11, 28 -- for {microscopy-at-microscopy.com} ; Thu, 29 Mar 2007 19:04:43 -0700 (PDT) 11, 28 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 11, 28 -- d=gmail.com; s=beta; 11, 28 -- h=domainkey-signature:received:received:message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 11, 28 -- b=pl9s2pP+b4Y4qHj3AdGx1YmquJPlE1Ao1mArtgFSP2+03M9OAHn0rd3hUMxz+YPhuQSgT1CZxeiORmsJFa808/B4My5IfcTi5Veyl03uPZCo+GpW8Gh0SeYM9DQPc8YV6uuSv4qH2PP2sjPqPK6bQHHjSGAHTl+hGr9jqrezIVI= 11, 28 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 11, 28 -- d=gmail.com; s=beta; 11, 28 -- h=received:message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 11, 28 -- b=KbXLKL2LFooWgFDXCFVHiBxDo1zS3VM4i+V1cWd9dWWLuSzZfiD7i0ZLLEW3h36n8F8zmWqqCdvNVq/lp59HhgfsIQYO/W9meeEy5jq2Rw+4N5cL3T+esfaYg+LB+06206km6P8fODbV08T3agEo/8FTUqeuK58PngfA/+m88JA= 11, 28 -- Received: by 10.100.139.9 with SMTP id m9mr980019and.1175220283599; 11, 28 -- Thu, 29 Mar 2007 19:04:43 -0700 (PDT) 11, 28 -- Received: by 10.100.32.16 with HTTP; Thu, 29 Mar 2007 19:04:43 -0700 (PDT) 11, 28 -- Message-ID: {ef580e610703291904n52d94aa7xc5acb74bd59aca3f-at-mail.gmail.com} 11, 28 -- Date: Thu, 29 Mar 2007 19:04:43 -0700 11, 28 -- From: "Bernard R. Cuzzillo, Ph.D., P.E." {bernard-at-berkeleyrc.com} 11, 28 -- Sender: biggerdadda-at-gmail.com 11, 28 -- To: microscopy-at-microscopy.com 11, 28 -- Subject: Jeol DSG1 Plus frame grabber 11, 28 -- MIME-Version: 1.0 11, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit 11, 28 -- Content-Disposition: inline 11, 28 -- X-Google-Sender-Auth: 89c52e2b5b44654f ==============================End of - Headers==============================
} The microscopes are an Olympus BH-2 and a Nikon EPIPHOT. } } 1) Does anyone have information or manuals on either of these } scopes that they would be willing to share?
You can find a catalogue and a manual for the BH-2 here:
Most of your questions will resolve themselves once you stop using the concept of "magnification". It's a fundamental issue: When people attach "databars" to images and insist on having the "magnification" number in there, this number will be wrong for every image size but the exact size at which it was taken. If you use a bigger monitor, or display the image with a projector, the magnification is changed.
A much better measure is "pixel size in nanometer" or "horizontal field width" etc.; these numbers are properties of the _image_ and not of the _display_.
So if you use a camera with a higher "resolution" (another term which is overloaded: it can mean both "number of pixels in a sensor" and "resolving power", but I mean "more pixels" here), you are simply able to have more pixels per nanometer of image data. It may not matter much for your screen, but printers typically have a much higher resolution (in the dpi meaning), so you'll be able to print out the same image with much more detail.
Your reasoning that you can also simply increase the magnification of the microscope only works so far. In physics, you can often check the validity of an assumption by trying "edge cases" (like infinite or zero). So, take a hypothetical CCD camera with only a single pixel. You can increase the magnification of your TEM until the cows come home, but you'll never be able to take an image with sufficient data to perform your post processing with.
If your post-processing software needs "1000 pixels" (say a 33x33 square) to recognize a feature, and you have a "one kilopixel camera", then you can only take pictures of one single feature at a time, if you make sure it fills the entire sensor. If you have a 1 megapixel camera, you need not be so accurate when acquiring the images as the software can crop out the interesting areas and still have plenty of pixels to do its recognition thing.
Let me float an question to the list. What is the effect of changing a one meg camera to a three meg camera on a TEM?
I'm asked some fundamental questions, and I can't seem to answer them to this person's satisfaction, which implies I don't have a good grasp of the question or answers.
My immediate response is you would increase the resolution. I can envision the image size on the monitor changing, but if the resolution of the screen is lower than the captured image and if the computer/imaging software wants to display all the image captured, will not any feature at a specific magnification have the resolution of the monitor? It seems the same is true for the printer. I can't simply expand the size of the paper at will, so the software will either reduce the printed image magnification or print just a smaller section of the total image. Again, since the printer has a fixed resolution will not the printed image resolution will be limited by the printer's (This sounds like a Hi-Fi discussion from the early 60's... just change the words...) upper limit?
So why capture high resolution images? My response is it allows you to post process and expand the image to examine one feature and have sufficient "stored" resolution to display the image without empty magnification. This also implies (to me at least) if I want to measure from point A to point B, the more camera pixels I have the better I can resolve where point A starts and point B ends which should allow me to have better confidence in my measurements. I believe that imaging software works on the image in memory and not the image displayed on the screen so size of the print or screen has little to do with data obtained. It's more a function of the size of the captured image?
Lastly.....
If I feel the need to have at least 1000 pixels in an image feature, and due to my camera I can only capture 500 at a magnification X, is there any reason not to simply increase the magnification so I have a larger feature which now occupies more pixels. I realize I haven't increased the resolution, but if my software need 999 pixels to "recognize" a feature haven't I met this requirement?
Thank in advance! Frank (I miss film) Karl
==============================Original Headers============================== 10, 17 -- From frank.karl-at-degussa.com Thu Mar 29 07:42:34 2007 10, 17 -- Received: from framailout1.rz.itson.com (mailout2.degussa.com [149.216.91.173]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2TCgXHD021519 10, 17 -- for {microscopy-at-msa.microscopy.com} ; Thu, 29 Mar 2007 07:42:33 -0500 10, 17 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [10.88.88.98]) 10, 17 -- by framailout1.rz.itson.com (8.13.4/8.13.4/Debian-3sarge3) with ESMTP id l2TCg2Bb031526 10, 17 -- for {microscopy-at-msa.microscopy.com} ; Thu, 29 Mar 2007 14:42:29 +0200 10, 17 -- Subject: Digital cameras and the TEM 10, 17 -- To: microscopy-at-msa.microscopy.com 10, 17 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 10, 17 -- Message-ID: {OF3BDC677E.DA558E0C-ON862572AD.003F9EC7-852572AD.0045C6B9-at-degussa.com} 10, 17 -- From: frank.karl-at-degussa.com 10, 17 -- Date: Thu, 29 Mar 2007 08:42:21 -0400 10, 17 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 10, 17 -- 03/29/2007 07:42:30 AM 10, 17 -- MIME-Version: 1.0 10, 17 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
==============================Original Headers============================== 23, 28 -- From Sander.Stoks-at-fei.com Fri Mar 30 03:28:55 2007 23, 28 -- Received: from smtp-nl1.feico.com (smtp-nl1.feico.com [195.75.179.210]) 23, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2U8Stg8000661 23, 28 -- for {microscopy-at-msa.microscopy.com} ; Fri, 30 Mar 2007 03:28:55 -0500 23, 28 -- X-WSS-ID: 0JFPKKR-02-368-01 23, 28 -- Received: from acht850.w2k.feico.com (unknown [10.150.55.50]) 23, 28 -- by smtp-nl1.feico.com (Tumbleweed MailGate) with SMTP id C670F10B9B18 23, 28 -- for {microscopy-at-msa.microscopy.com} ; Fri, 30 Mar 2007 01:50:50 -0700 (PDT) 23, 28 -- Received: From acht887.w2k.feico.com ([10.150.55.87]) by acht850.w2k.feico.com (WebShield SMTP v4.5 MR2); 23, 28 -- id 1175243331545; Fri, 30 Mar 2007 10:28:51 +0200 23, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 23, 28 -- Content-class: urn:content-classes:message 23, 28 -- MIME-Version: 1.0 23, 28 -- Content-Type: text/plain; 23, 28 -- charset="iso-8859-1" 23, 28 -- Subject: RE: [Microscopy] Digital cameras and the TEM 23, 28 -- Date: Fri, 30 Mar 2007 10:28:51 +0200 23, 28 -- Message-ID: {9211E4E38C8130428654AD6A1081A2CD0BF4EFF4-at-acht887.w2k.feico.com} 23, 28 -- X-MS-Has-Attach: 23, 28 -- X-MS-TNEF-Correlator: 23, 28 -- Thread-Topic: [Microscopy] Digital cameras and the TEM 23, 28 -- Thread-Index: AcdyAikZDYrHCQWeT4SIL9becQqp0wAoV2fi 23, 28 -- References: {200703291249.l2TCnb1D031617-at-ns.microscopy.com} 23, 28 -- From: "Stoks, Sander" {Sander.Stoks-at-fei.com} 23, 28 -- To: {microscopy-at-msa.microscopy.com} 23, 28 -- Cc: {frank.karl-at-degussa.com} 23, 28 -- Content-Transfer-Encoding: 8bit 23, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l2U8Stg8000661 ==============================End of - Headers==============================
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Email: dax_uktc-at-abv.bg Name: Yordan Naydenov
Organization: Faculty of Biology, University of Sofia
Our lab obtained a Reichert Zetopan microscope (combined with Binolux III twin lamp unit). Unfortunately, we don't have any instruction/exploitation manuals both for the microscope and the unit. I have searched the archives of this site (http://www.microscopy.com) and found that others also had asked for such manuals. Some people had put materials in the web space but this sources are not available at present. Could anyone provide us with such documents (in electronic format or xeroxed; preferably in English)? If so, please, contact me at dax_uktc-at-abv.bg to discuss the details of submitting.
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Email: rachid_sakly-at-yahoo.fr Name: rachid
Organization: Ecole Sup des Sci et Tech de la SantÈ
Title-Subject: [Filtered] sodium rhodizonate test
Question: I need to know the details of sodium rhodizonate test allowing to highlight lead crystals in testes and other organs in rats.
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Email: MTLab-at-comcast.net Name: Roy Nelson
Organization: Material Testing Laboratory
Title-Subject: [Filtered] Manual for Robinson Detector
Question: We have started using a Robinson detector (model RDEM II) that came with a used SEM. A manual would great either in electronic or paper form. I have already searched the archives and sent an E-mail to SEMRA. Please contact me directly at MTLab-at- comcast.net or (609) 730-0575.
The Commission on Electron Diffraction of the IUCR has setup a web page which contains several things which may be of interest to members of this list:
1) A listing of software for electron microscopy at http://www.numis.northwestern.edu/IUCR_CED/software/html/ecsoftware.htm The idea is that people can add their software to this list, which is currently rather small.
2) Some information about different techniques at http://www.numis.northwestern.edu/IUCR_CED/details.htm At the moment this is very short -- contributions welcome.
3) A short list of upcoming conferences; please send information to my real email (in signature) to add.
Other information will be added in the future, for instance how to apply for IUCR support for workshops or schools on electron crystallography.
-- Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2220 N Campus Drive Northwestern University Evanston, IL 60208, USA Tel: (847) 491-3996 Fax: (847) 491-7820 email: L-marks at northwestern dot edu Web: www.numis.northwestern.edu EMM2007 http://ns.crys.ras.ru/EMMM07/ Commission on Electron Diffraction of IUCR www.numis.northwestern.edu/IUCR_CED
==============================Original Headers============================== 6, 26 -- From marksmsa-at-gmail.com Fri Mar 30 09:22:48 2007 6, 26 -- Received: from ug-out-1314.google.com (ug-out-1314.google.com [66.249.92.175]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2UEMjRO020800 6, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Mar 2007 09:22:47 -0500 6, 26 -- Received: by ug-out-1314.google.com with SMTP id m2so824918ugc 6, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Mar 2007 07:22:44 -0700 (PDT) 6, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 6, 26 -- d=gmail.com; s=beta; 6, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 6, 26 -- b=esqVwWUwfQ/FGY0D15TjAJKIF5qY+ToJ9MuJeNi/VW6JjZtlz8Ke+yXUxY6hO8AZRyst9R5N7SK43ZkM0xI3c/hLcY7LmbSRvl87EAQwzJRvEbUKVK1obCssvVUnNbmQg4vgOQD8h7nyfAK7iwdrfPaM1tZibD5XY99rZJP77Cc= 6, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 26 -- d=gmail.com; s=beta; 6, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 6, 26 -- b=l0Uo0zD1RzaGPrvhrPTXuUqu+xdJrHImqWJfKEiAGsRS6f1KlIzufhXSV0hUmYIWWF9nH8TEGX/ywFR1YMLomRlYdpTAoZpWKBQOv8UCvZlorzUwIVU9CrGIQjHnFrT7pkHnf93JAs2sHsAzAGXYxCqAGXM9KAmfUt2ewHMF1Zo= 6, 26 -- Received: by 10.78.124.13 with SMTP id w13mr887677huc.1175264564243; 6, 26 -- Fri, 30 Mar 2007 07:22:44 -0700 (PDT) 6, 26 -- Received: by 10.78.52.13 with HTTP; Fri, 30 Mar 2007 07:22:44 -0700 (PDT) 6, 26 -- Message-ID: {e13ba6260703300722w76fcb75av7ede9f44e3950c7f-at-mail.gmail.com} 6, 26 -- Date: Fri, 30 Mar 2007 09:22:44 -0500 6, 26 -- From: "L Marks" {marksmsa-at-gmail.com} 6, 26 -- To: Microscopy-at-microscopy.com 6, 26 -- Subject: Electron Microscopy Software Listing 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 26 -- Content-Transfer-Encoding: 7bit 6, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
I have some of these manuals (also, a Zetapan which I treasure!), but will need some time to find them. When I got my Zetapan (in the early 1980's) I had a chance to raid the Reichert office in Slough, England, for manuals, etc.
I will be out of the office until next Tuesday afternoon. If you email me next week, I'll see what I can do for you.
Best regards,
Barbara Foster, President
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
MME is now scheduling customized, on-site courses through July. Call us today for details.
P. S. Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 12:01 PM 3/30/2007, dax_uktc-at-abv.bg wrote:
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==============================Original Headers============================== 16, 17 -- From bfoster-at-mme1.com Fri Mar 30 13:06:56 2007 16, 17 -- Received: from 5starpro.com (enterprise.5starpro.com [207.44.136.95]) 16, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2UI6rbt004988 16, 17 -- for {microscopy-at-microscopy.com} ; Fri, 30 Mar 2007 13:06:55 -0500 16, 17 -- Message-Id: {200703301806.l2UI6rbt004988-at-ns.microscopy.com} 16, 17 -- Received: (qmail 2066 invoked by uid 2020); 30 Mar 2007 13:39:16 -0500 16, 17 -- Received: from host57-99.rancor.birch.net (HELO barbsd505.mme1.com) (65.17.57.99) 16, 17 -- by enterprise.5starpro.com with (DHE-RSA-AES256-SHA encrypted) SMTP; 30 Mar 2007 13:39:15 -0500 16, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 16, 17 -- Date: Fri, 30 Mar 2007 13:06:33 -0600 16, 17 -- To: dax_uktc-at-abv.bg, microscopy-at-microscopy.com 16, 17 -- From: Barbara Foster {bfoster-at-mme1.com} 16, 17 -- Subject: Re: [Microscopy] viaWWW: Reichert Zetopan Manuals 16, 17 -- In-Reply-To: {200703301247.l2UClLpJ024546-at-ns.microscopy.com} 16, 17 -- References: {200703301247.l2UClLpJ024546-at-ns.microscopy.com} 16, 17 -- Mime-Version: 1.0 16, 17 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
I have Zetopan manuals you can download free at www.science-info.net/docs/reichert {http://www.science-info.net/docs/reichert/} I believe I have both English and German copays there.
I collect manuals for old microscope and related equipment and keep for download at: www.science-info.net {http://www.science-info.net}
Most of the manuals come from others in a group effort to make the available for all.
Gordon Stillwater OK
dax_uktc-at-abv.bg wrote: } } Email: dax_uktc-at-abv.bg } Name: Yordan Naydenov } } Organization: Faculty of Biology, University of Sofia } } Title-Subject: [Filtered] Reichert Zetopan Manuals } } Question: Hello, } } Our lab obtained a Reichert Zetopan microscope (combined with Binolux III twin lamp unit). Unfortunately, we don't have any instruction/exploitation manuals both for the microscope and the unit. } I have searched the archives of this site (http://www.microscopy.com) and found that others also had asked for such manuals. Some people had put materials in the web space but this sources are not available at present. } Could anyone provide us with such documents (in electronic format or xeroxed; preferably in English)? If so, please, contact me at dax_uktc-at-abv.bg to discuss the details of submitting. } } Thanks in advance, } Yordan Naydenov } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Fri Mar 30 07:44:12 2007 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l2UCiAT6016906 } 8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 30 Mar 2007 07:44:11 -0500 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240805c232b652fa64-at-[206.69.208.22]} } 8, 11 -- Date: Fri, 30 Mar 2007 07:44:10 -0500 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: dax_uktc-at-abv.bg (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: Reichert Zetopan Manuals } 8, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== } }
==============================Original Headers============================== 8, 19 -- From gcouger-at-science-info.net Fri Mar 30 15:35:06 2007 8, 19 -- Received: from smtp106.biz.mail.re2.yahoo.com (smtp106.biz.mail.re2.yahoo.com [206.190.52.175]) 8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2UKZ60B019622 8, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Mar 2007 15:35:06 -0500 8, 19 -- Received: (qmail 63057 invoked from network); 30 Mar 2007 20:35:05 -0000 8, 19 -- Received: from unknown (HELO ?192.168.1.115?) (gcouger-at-science-info.net-at-74.195.225.38 with plain) 8, 19 -- by smtp106.biz.mail.re2.yahoo.com with SMTP; 30 Mar 2007 20:35:05 -0000 8, 19 -- X-YMail-OSG: Q1d9r1sVM1l_0yErQaRbSHE6owvxhxdFpBVf8OEtFgkzQiehVDh3VXHxlYDSVm.LJKp2Ltnr3ZndLf.CpAhqF3Ww7_F6xs7CYaXXW9S5vUqW17GPcxqY05FYcg1f7TM5jUBXhWq6hwCkYwzRtmFGsolE_qHwnXv2IemwXoFBj2HM 8, 19 -- Message-ID: {460D7488.1030909-at-science-info.net} 8, 19 -- Date: Fri, 30 Mar 2007 15:35:20 -0500 8, 19 -- From: Gordon Couger {gcouger-at-science-info.net} 8, 19 -- User-Agent: Thunderbird 1.5.0.10 (Macintosh/20070221) 8, 19 -- MIME-Version: 1.0 8, 19 -- To: dax_uktc-at-abv.bg, Microscopy-at-microscopy.com 8, 19 -- Subject: Re: [Microscopy] viaWWW: Reichert Zetopan Manuals 8, 19 -- References: {200703301254.l2UCs9rj006515-at-ns.microscopy.com} 8, 19 -- In-Reply-To: {200703301254.l2UCs9rj006515-at-ns.microscopy.com} 8, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: maloneyb-at-fiu.edu Name: Barbara
Organization: FIU
Title-Subject: [Filtered] Analoug video time lapse
Question: Dear Group - has anyone had success in connecting a analog video camera to a light scope for time lapse studies? I know the way to go is digital, however, I'm hoping to improvise. Any suggestions would be greatly appreciated. Thanks Barbara
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Email: slc6-at-lehigh.edu Name: Sharon Coe
Organization: Lehigh University
Title-Subject: [Filtered] Lehigh University Microscopy School
Question: There is still time to register for the 2007 Lehigh Microscopy School. This will be the 37th year of course offerings which include:
SEM and X-ray Microanalysis (June 4-8)
Introduction to SEM and EDS (June 3)
Scanning Probe Microscopy: From Fundamentals to Advanced Applications (June 4-8)
Quantitative X-ray Microanalysis of Bulk Specimens and Particles (June 11-15)
Problem Solving with SEM, X-ray Microanalysis, and Backscatter Patterns (June 11-15)
Anaytical Electron Microscopy at the Nanometer Scale (June 11-14)
Focused Ion Beam Instrumentaion and Appplications (June 11-14)
For more information please contact Sharon Coe Sharon.coe-at-lehigh.edu 610-758-5133 www.lehigh.edu/microscopy
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Email: MTLab-at-comcast.net Name: Roy Nelson
Organization: Material Testing Laboratory
Title-Subject: [Filtered] Manual for Robinson Detector
Question: Thanks for the quick response. I have copy of a manual being mail to me on Monday.
It would seem quite possible to use an NTSC color or b/w TV camera and then frame grab with a PCI card. These are quite low cost. The software for it would need to allow lime lapse capture or .avi which can be edited. So be sure to check the software app to see if it will do time lapse or streaming capture rather than just one frame at a time.
gary g.
At 02:43 PM 3/30/2007, you wrote:
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==============================Original Headers============================== 9, 20 -- From gary-at-gaugler.com Fri Mar 30 17:59:58 2007 9, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l2UMxwtm004220 9, 20 -- for {microscopy-at-microscopy.com} ; Fri, 30 Mar 2007 17:59:58 -0500 9, 20 -- Message-Id: {200703302259.l2UMxwtm004220-at-ns.microscopy.com} 9, 20 -- Received: (qmail 1354 invoked from network); 30 Mar 2007 15:59:58 -0700 9, 20 -- Received: by simscan 1.1.0 ppid: 1349, pid: 1351, t: 0.1016s 9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 9, 20 -- by qsmtp3 with SMTP; 30 Mar 2007 15:59:58 -0700 9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 20 -- Date: Fri, 30 Mar 2007 15:59:57 -0800 9, 20 -- To: maloneyb-at-fiu.edu 9, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 20 -- Subject: Re: [Microscopy] viaWWW: Analoug video time lapse 9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 20 -- In-Reply-To: {200703302243.l2UMhQPK011039-at-ns.microscopy.com} 9, 20 -- References: {200703302243.l2UMhQPK011039-at-ns.microscopy.com} 9, 20 -- Mime-Version: 1.0 9, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-5363425 ==============================End of - Headers=============================
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