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Email: modla-at-dbi.udel.edu Name: Shannon Modla
Organization: Delaware Biotechnology Institute
Title-Subject: [Filtered] Metal Shadow Casting of DNA
Question: We are currently trying to rotary shadow cast DNA but are having problems obtaining consistent results with the metal shadowing. We are using an 80:20 platinum palladium wire purchased from EMS and a Denton Bench Top Turbo III sputter coater. The platinum palladium wire was wrapped around a 2-3 cm portion of a hairpin loop formed with tungsten wire. When the filament power is increased, the platinum palladium wire will either heat up without melting and evaporating or else the tungsten wire breaks without appreciable metal shadowing. I was wondering if anyone had any experience with this technique and could offer suggestions about how to obtain more consistent results.
Thanks,
Shannon Modla Delware Biotechnology Institute BioImaging Center
The geology department is looking for recommendation on getting a Cathodoluminescence-Optical Microscope for geological research purpose. Samples to be view would include but not limited to Zircon, Quartz, and carbonates. We appreciate any recommendations and experiences you may have on such instrumentation. Thanks in advance!
Best Regards, ---------------------------------------------- Xiang Yang Electron Microscopy Center Saint Mary's University Science Building, Suite 135 Halifax, NS Canada B3H 3C3 Tel: (902) 496-8292 (lab) (902) 420-5709 (off) http://fgsr.smu.ca/emc/
==============================Original Headers============================== 4, 21 -- From xyang-at-SMU.CA Fri Jun 1 08:32:08 2007 4, 21 -- Received: from HUSKY8.SMU.CA (Husky8.smu.ca [140.184.1.8]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51DW7HP007335 4, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 08:32:08 -0500 4, 21 -- Received: from s101tech ("port 4931"-at-[140.184.73.254]) 4, 21 -- by HUSKY1.SMU.CA (PMDF V6.2-X25 #30841) 4, 21 -- with SMTP id {01MH9S50LTZC8Z2TIS-at-HUSKY1.SMU.CA} for Microscopy-at-microscopy.com; 4, 21 -- Fri, 01 Jun 2007 10:32:06 -0300 4, 21 -- Date: Fri, 01 Jun 2007 10:31:38 -0300 4, 21 -- From: Xiang Yang {xyang-at-SMU.CA} 4, 21 -- Subject: vendors on CL-microscope 4, 21 -- To: Microscopy-at-microscopy.com 4, 21 -- Reply-to: Xiang Yang {xyang-at-SMU.CA} 4, 21 -- Message-id: {007b01c7a451$405ab0a0$fe49b88c-at-stmarys.smunet.smu.ca} 4, 21 -- MIME-version: 1.0 4, 21 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 4, 21 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3028 4, 21 -- Content-type: text/plain; reply-type=original; charset=iso-8859-1; format=flowed 4, 21 -- Content-transfer-encoding: 7bit 4, 21 -- X-Priority: 3 4, 21 -- X-MSMail-priority: Normal ==============================End of - Headers==============================
Our UC-4 has never been re-evacuated. It has a yellow plastic cap over the port, the port is filled with a pink grease, and under the pink grease it looks like a metal ball. I can't say any more, but it looks like the metal ball is original.
Diane Ciaburri
-----Original Message----- X-from: oddioeng-at-aol.com [mailto:oddioeng-at-aol.com] Sent: Thursday, May 31, 2007 10:53 PM To: Ciaburri, Diane A.
What for did you "spin 200nm PMMA on the surface?" As I understand, you have just glassy flat surface of resin on your specimen, with no topography and nothing to observe.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} } Email: yitianp-at-ece.arizona.edu } Name: Yitian Peng } } Organization: University of Arizona } } Title-Subject: [Filtered] Question about image on Glass. } } Question: Hi everyone, } I have some patterned Cr structure(width:100micro, } height:60nm) on Glass. } Then I spin 200nm PMMA on the surface. } Then I Sputter 10nm Cr on the PMMA. } I want to using JSM 6400 SEM to look at the structure. } But I can't see anything?/ } What's the problem?/ } Thank you very much. } Yitian } } } -------------------------------------------------------------- } ------------- } } ==============================Original } Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Thu May 31 21:52:36 2007 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id l512qZ3N011694 } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 31 May } 2007 21:52:35 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p0624080ac2853a635b12-at-[206.69.208.22]} } 7, 11 -- Date: Thu, 31 May 2007 21:52:34 -0500 7, 11 -- To: } microscopy-at-microscopy.com 7, 11 -- From: } yitianp-at-ece.arizona.edu (by way of MicroscopyListserver) 7, } 11 -- Subject: viaWWW: Question about image on Glass 7, 11 -- } Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers============================== }
==============================Original Headers============================== 6, 25 -- From DusevichV-at-umkc.edu Fri Jun 1 08:54:07 2007 6, 25 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51Ds7jf030569 6, 25 -- for {microscopy-at-msa.microscopy.com} ; Fri, 1 Jun 2007 08:54:07 -0500 6, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.1830); 6, 25 -- Fri, 1 Jun 2007 08:54:07 -0500 6, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 25 -- Content-class: urn:content-classes:message 6, 25 -- MIME-Version: 1.0 6, 25 -- Content-Type: text/plain; 6, 25 -- charset="us-ascii" 6, 25 -- Subject: RE: [Microscopy] viaWWW: Question about image on Glass 6, 25 -- Date: Fri, 1 Jun 2007 08:54:06 -0500 6, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB33ADCAA-at-KC-MSX1.kc.umkc.edu} 6, 25 -- In-Reply-To: {200706010253.l512rpk7013748-at-ns.microscopy.com} 6, 25 -- X-MS-Has-Attach: 6, 25 -- X-MS-TNEF-Correlator: 6, 25 -- Thread-Topic: [Microscopy] viaWWW: Question about image on Glass 6, 25 -- Thread-Index: Acej+Bjmiqw1+i/PT7aFRDMN0xOWGQAW3m3w 6, 25 -- References: {200706010253.l512rpk7013748-at-ns.microscopy.com} 6, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 6, 25 -- To: {yitianp-at-ece.arizona.edu} , {microscopy-at-msa.microscopy.com} 6, 25 -- X-OriginalArrivalTime: 01 Jun 2007 13:54:07.0130 (UTC) FILETIME=[527A3FA0:01C7A454] 6, 25 -- Content-Transfer-Encoding: 8bit 6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l51Ds7jf030569 ==============================End of - Headers==============================
I'm not entirely sure if this is appropriate for this list, and if it isn't I apologize in advance. I was just informed yesterday that my position as science teacher at Atlantis Academy has been eliminated, and that the math teacher will be teaching both subjects. It is unfortunately a sad commentary on the direction that our world is headed in.
I am making an appeal to anyone in the south Florida area who may need some extra lab help, or anything that I can do. My prospects for getting a new teaching job are not good until later in the summer, but I would really like to start working somewhere locally as a lab assistant so I can start working on my masters degree.
I know this is a bit of a shot in the dark, but I figured there'd be no harm in asking.
Thank you all for your kind support over the last few months on our SEM project. I am truly sorry that I will not be able to see it come to fruition.
--Justin A. Kraft
==============================Original Headers============================== 5, 26 -- From kraftpiano-at-gmail.com Fri Jun 1 09:06:48 2007 5, 26 -- Received: from nz-out-0506.google.com (nz-out-0506.google.com [64.233.162.233]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51E6ltZ009776 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 09:06:48 -0500 5, 26 -- Received: by nz-out-0506.google.com with SMTP id m7so462802nzf 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 01 Jun 2007 07:06:47 -0700 (PDT) 5, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 5, 26 -- d=gmail.com; s=beta; 5, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 26 -- b=qs9vE5p9pcEXK/Msl9ElX2ktGlmc8jozq5wszsRSWN6ArbUwJLxxunGvssS2tFtDtss14oERxMeGxC8fTGBaTlND/3bD+Vg5XHffF3pR37edRsswSjQ7LI4Szh9Tvq3pT3wHKR8m1T6QOUn6G9faTwZdu/ZbAAmmuFP0/iV+P9E= 5, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 26 -- d=gmail.com; s=beta; 5, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 26 -- b=JqpAB/S5rMJwRtokakle1G6MLLcW4qbSMLkqmmmV3QgTez0J6QNOS6g2A2gW3T6rUw6y2F1MQiyWxmxjafDGdQzC7+GBxAA+KeINaSr9z8iDh+phCl1cO4lkBTjEKFPnrJ7VFK1DhociSHCDTrabJMf9nVLRJkflzXEhpqGouYQ= 5, 26 -- Received: by 10.114.148.1 with SMTP id v1mr1863566wad.1180706807173; 5, 26 -- Fri, 01 Jun 2007 07:06:47 -0700 (PDT) 5, 26 -- Received: by 10.114.78.15 with HTTP; Fri, 1 Jun 2007 07:06:46 -0700 (PDT) 5, 26 -- Message-ID: {25e2b0d20706010706r5763fc81gfb6396e2d59d09c7-at-mail.gmail.com} 5, 26 -- Date: Fri, 1 Jun 2007 10:06:46 -0400 5, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 5, 26 -- To: microscopy-at-microscopy.com 5, 26 -- Subject: Unfortunate announcement. 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
I was once given the task of looking at a glassy surface in the SEM. Just to rough in the focus was a challenge. I sprinkled some powder on the surface to provide a starting point for focus. Maybe this would help you get started.
Otherwise, I agree with Vladimir... that PMMA - though optically transparent - is not electron transparent, and all you'll see is the Cr-sputter-coated polymer surface.
Stu Smalinskas, P.E. Metallurgist SKF USA Plymouth, Michigan (734)414-6862
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Vladimir wrote:
What for did you "spin 200nm PMMA on the surface?" As I understand, you have just glassy flat surface of resin on your specimen, with no topography and nothing to observe.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
Title-Subject: [Filtered] Question about image on Glass.
Question: Hi everyone, I have some patterned Cr structure (width: 100micro, height: 60nm) on Glass. Then I spin 200nm PMMA on the surface. Then I Sputter 10nm Cr on the PMMA. I want to using JSM 6400 SEM to look at the structure. But I can't see anything? What's the problem?
Thank you very much.
Yitian
Email: yitianp-at-ece.arizona.edu Name: Yitian Peng Organization: University of Arizona
____________________________________________________________________________________ Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. http://answers.yahoo.com/dir/?link=list&sid=396546091
==============================Original Headers============================== 18, 19 -- From smalinskas-at-yahoo.com Fri Jun 1 09:44:16 2007 18, 19 -- Received: from web34402.mail.mud.yahoo.com (web34402.mail.mud.yahoo.com [66.163.178.151]) 18, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l51EiFbR022834 18, 19 -- for {microscopy-at-sparc5.microscopy.com} ; Fri, 1 Jun 2007 09:44:16 -0500 18, 19 -- Received: (qmail 49157 invoked by uid 60001); 1 Jun 2007 14:44:13 -0000 18, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 18, 19 -- s=s1024; d=yahoo.com; 18, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 18, 19 -- b=ohxTk6rrG9wPMmpEB41srU7p0itpjgVWE9ku/z16opwRMjvNQkPlCv/FugD4wBXDK/YdUZtq8Fe2ynDWSgR08V//oypLZTuy/NjQ0tdAuvFMbqplyM0zeUApL4EskTkDZ7yZHN4XAK7a3eTKe1jsohcONgRn+NKQuOl4xzrnLco=; 18, 19 -- X-YMail-OSG: FtqzHRoVM1mN3BbnjqYQIes90SQ7DzVLUM7AHHe.Y6LKZ5SuJ0ttL5NLQuSwS3nVsIIpObInQHE6fWq_f1ElpwaB0A4nI04bvBUruRmTrj7lGyTjiEzR9j.BWwBTsA-- 18, 19 -- Received: from [141.151.33.213] by web34402.mail.mud.yahoo.com via HTTP; Fri, 01 Jun 2007 07:44:13 PDT 18, 19 -- Date: Fri, 1 Jun 2007 07:44:13 -0700 (PDT) 18, 19 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 18, 19 -- Subject: [Microscopy] RE: viaWWW: Question about image on Glass 18, 19 -- To: microscopy-at-ns.microscopy.com, yitianp-at-ece.arizona.edu 18, 19 -- MIME-Version: 1.0 18, 19 -- Content-Type: text/plain; charset=iso-8859-1 18, 19 -- Content-Transfer-Encoding: 8bit 18, 19 -- Message-ID: {213428.48556.qm-at-web34402.mail.mud.yahoo.com} ==============================End of - Headers==============================
I don't know the particulars of operation of the evaporator unit and my knowledge is for pure gold or platinum (some metals will alloy with the tungsten and cause problems - maybe someone else knows).
Platinum MP = 1772 C Tungsten MP = 3410 C
If the Pt wire is not melting, then the tungsten temperature is too low. If you advance the filament heating slowly (a few seconds for work, but slower in testing until you know the behaviour) you should first see the Pt:Pd wire melt and flow to the bottom of the hairpin (the point of the V is down) and the droplet will evaporate at a slightly higher temperature. Actually you only need to put the wire near the tip of the V.
Just remember to heat the filament over a few seconds, not instantly. Tungsten has a positive temperature coefficient and the filament resistance is initially low and a large surge will occur if full voltage is applied directly - it can cause the evaporant wire to spit or the filament to burn out; but you shouldn't need to be that close to the tungten melting point.
You don't specify distances but it sounds like you are using a lot of wire. You probably only need 10-20Å. 0.5 cm of 8 mil wire at 10 cm distance will give ~13 Å (at normal incidence, or the sides of DNA so disposed....)
Å = (40.3 * diameter^2 * Length) / distance^2
diameter in "mils" (0.001"), 8 mils is a standard size, 0.008" dia length of wire evaporant, distance to sample in cm
I have an Excel spreadsheet with a "calculator" for this formula that I can send if you want - can't go to the Microscopy Listserver..... Just ask.
Hope this helps.
Dale Callaham Univ. of Massachusetts, Amherst
modla-at-dbi.udel.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://ns.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both modla-at-dbi.udel.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: modla-at-dbi.udel.edu } Name: Shannon Modla } } Organization: Delaware Biotechnology Institute } } Title-Subject: [Filtered] Metal Shadow Casting of DNA } } Question: We are currently trying to rotary shadow cast DNA but are having problems obtaining consistent results with the metal shadowing. We are using an 80:20 platinum-palladium wire purchased from EMS and a Denton Bench Top Turbo III sputter coater. The platinum palladium wire was wrapped around a 2-3 cm portion of a hairpin loop formed with tungsten wire. When the filament power is increased, the platinum palladium wire will either heat up without melting and evaporating or else the tungsten wire breaks without appreciable metal shadowing. I was wondering if anyone had any experience with this technique and could offer suggestions about how to obtain more consistent results. } } Thanks, } } Shannon Modla } Delware Biotechnology Institute } BioImaging Center } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Fri Jun 1 07:50:13 2007 } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51CoCCh027317 } 9, 11 -- for {microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 07:50:13 -0500 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240815c285c66f2e87-at-[206.69.208.22]} } 9, 11 -- Date: Fri, 1 Jun 2007 07:50:12 -0500 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: modla-at-dbi.udel.edu (by way of MicroscopyListserver) } 9, 11 -- Subject: viaWWW: Metal Shadow Casting of DNA } 9, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
==============================Original Headers============================== 14, 21 -- From dac-at-research.umass.edu Fri Jun 1 09:55:15 2007 14, 21 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40]) 14, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51EtEr4002073 14, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 09:55:15 -0500 14, 21 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 14, 21 -- (authenticated bits=0) 14, 21 -- by race4.oit.umass.edu (8.13.7/8.13.7) with ESMTP id l51EtCWW031954 14, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 14, 21 -- Fri, 1 Jun 2007 10:55:13 -0400 14, 21 -- Message-ID: {466041E0.4080300-at-research.umass.edu} 14, 21 -- Date: Fri, 01 Jun 2007 10:57:20 -0500 14, 21 -- From: Dale Callaham {dac-at-research.umass.edu} 14, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.2) Gecko/20070222 SeaMonkey/1.1.1 14, 21 -- MIME-Version: 1.0 14, 21 -- To: modla-at-dbi.udel.edu, Microscopy Listserver {Microscopy-at-microscopy.com} 14, 21 -- Subject: Re: [Microscopy] viaWWW: Metal Shadow Casting of DNA 14, 21 -- References: {200706011256.l51Cuoup004737-at-ns.microscopy.com} 14, 21 -- In-Reply-To: {200706011256.l51Cuoup004737-at-ns.microscopy.com} 14, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 21 -- Content-Transfer-Encoding: 8bit 14, 21 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Sorry to occupy your mailbox, but I've had a couple of posts that didn't. Ken
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
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==============================Original Headers============================== 7, 27 -- From kenconverse-at-qualityimages.biz Fri Jun 1 10:14:02 2007 7, 27 -- Received: from dpmailmta02.doteasy.com (dpmailmta02.doteasy.com [65.61.209.90]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51FE2Gt013936 7, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 1 Jun 2007 10:14:02 -0500 7, 27 -- Received: from qualityimages.biz (unverified [192.168.101.16]) 7, 27 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id 80292733-1814644 7, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 01 Jun 2007 10:18:13 -0700 7, 27 -- Received: from Ken [72.227.100.25] by qualityimages.biz with ESMTP 7, 27 -- (SMTPD32-8.05) id A7B342760052; Fri, 01 Jun 2007 08:13:55 -0700 7, 27 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 7, 27 -- To: "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 7, 27 -- Subject: test 7, 27 -- Date: Fri, 1 Jun 2007 11:13:39 -0400 7, 27 -- Message-ID: {001d01c7a45f$71f97250$6401a8c0-at-Ken} 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="us-ascii" 7, 27 -- Content-Transfer-Encoding: 7bit 7, 27 -- X-Priority: 3 (Normal) 7, 27 -- X-MSMail-Priority: Normal 7, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 7, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 7, 27 -- Thread-Index: AcekX262nmEQmKvJQ2OPBpGYnI1zQA== 7, 27 -- Importance: Normal 7, 27 -- X-IMSTrailer: __IMail_7__ 7, 27 -- X-IP-stats: Incoming Last 0, First 246, in=4561928, out=0, spam=0 ip=192.168.101.16 7, 27 -- X-Originating-IP: 192.168.101.16 ==============================End of - Headers==============================
I have recently had a power outage and when I re-started my JEOL 6400 equipped OXFORD INCA X-sight the INCA software gave me an error stating "(1426)DSPP Failed Board" and my deadtime is 99% even with no beam. Can anyone help? Are the two errors related? Thanks Kirk
==============================Original Headers============================== 2, 17 -- From kross-at-laurentian.ca Fri Jun 1 10:15:09 2007 2, 17 -- Received: from lugwout.laurentian.ca (lugwout.laurentian.ca [142.51.1.65]) 2, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51FF9tf015968 2, 17 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 10:15:09 -0500 2, 17 -- Received: from OUTDOM-MTA by lugwout.laurentian.ca 2, 17 -- with Novell_GroupWise; Fri, 01 Jun 2007 11:15:08 -0400 2, 17 -- Message-Id: {465FFF8E0200005200004526-at-lugwout.laurentian.ca} 2, 17 -- X-Mailer: Novell GroupWise Internet Agent 7.0.1 2, 17 -- Date: Fri, 01 Jun 2007 11:14:43 -0400 2, 17 -- From: "Kirk Ross" {kross-at-laurentian.ca} 2, 17 -- To: {Microscopy-at-microscopy.com} 2, 17 -- Subject: Oxford, EDS deadtime 2, 17 -- Mime-Version: 1.0 2, 17 -- Content-Type: text/plain; charset=US-ASCII 2, 17 -- Content-Disposition: inline 2, 17 -- Content-Transfer-Encoding: 8bit 2, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l51FF9tf015968 ==============================End of - Headers==============================
They might be related. However, first thing to do is to home the stage. There should be an option in the SEM control to initialize the stage. Do this. If the stage uses LEDs, this will drive the EDS nuts with high cps and high DT. Restart PC.
If this does not fix the problem, then you might have a damaged board from the power outage.
gary g.
At 07:16 AM 6/1/2007, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Fri Jun 1 11:06:05 2007 10, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l51G64PD012957 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 11:06:05 -0500 10, 20 -- Message-Id: {200706011606.l51G64PD012957-at-ns.microscopy.com} 10, 20 -- Received: (qmail 6663 invoked from network); 1 Jun 2007 08:39:23 -0700 10, 20 -- Received: by simscan 1.1.0 ppid: 6660, pid: 6661, t: 0.0919s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp3 with SMTP; 1 Jun 2007 08:39:23 -0700 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Fri, 01 Jun 2007 08:39:38 -0800 10, 20 -- To: kross-at-laurentian.ca 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] Oxford, EDS deadtime 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200706011516.l51FGYCv021250-at-ns.microscopy.com} 10, 20 -- References: {200706011516.l51FGYCv021250-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-52C91BC8 ==============================End of - Headers==============================
Energy Beam Sciences is the US distributor for Quorum Technologies, which handles both the Polaron, and Emitech line of preparation equipment, including Critical Point Dryers.
Quorum offers both a horizontal and vertical style CPD system, with the horizontal style the user has the advantage of working with small or large samples based on the chamber set-up, with the vertical system offering more of the automatic features.
I'll look to contact you off-line to share details on the systems and your needs.
Regards,
Mike Dufraine Energy Beam Sciences,Inc.
mdienelt-at-msn.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mdienelt-at-msn.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mdienelt-at-msn.com } Name: Margaret Dienelt } } Organization: USDA, ARS, National Arboretum } } Title-Subject: [Filtered] Advice on critical point dryers } } Question: Hello everyone, } } I've just learned we might have funds to replace our ancient, } tempermental critical point dryer and would greatly appreciate any } feedback anyone can give me on their CPD. I'm especially interested } in reliability - the valves in our current unit have been a problem } for years, requiring numerous repairs (usually after destroying a few } samples). } } In addition to information anyone can share on specific models, I } also have two general questions: } } What the pros and cons are to the two profiles, i.e. horizontal (like } Tousimis) and vertical (like Polaron)? } } What are pros and cons to automatic vs manual models? } } Basically, any wisdom you'd like to share about purchasing a new } critical point dryer will be more than welcome. Our new one will be } used in a plant pathology lab and will be used to process primarily } plant tissue. We don't need one for a clean room or one with the } jumbo chamber. } } I'm sending this from my personal e-mail account since I'm having } problems with my government e-mail, but if replies are sent to } mdienelt-at-msn.com or margaret.dienelt-at-ars.usda.gov. I'll receive them } one way or the other. } } Thank you! } } Margaret } } } } Margaret Dienelt } Plant Pathologist/Electron Microscopist } Floral and Nursery Plants Research Unit } National Arboretum, ARS, USDA } } 10300 Baltimore Avenue } Beltsville, MD 20705 } } (301)504-6097 } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 20, 11 -- From zaluzec-at-microscopy.com Thu May 31 15:08:33 2007 } 20, 11 -- Received: from [165.68.138.44] (msdvpn8.msd.anl.gov [130.202.238.72]) } 20, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l4VK8WMU025246 } 20, 11 -- for {microscopy-at-microscopy.com} ; Thu, 31 May 2007 15:08:33 -0500 } 20, 11 -- Mime-Version: 1.0 } 20, 11 -- Message-Id: {p06240800c284dbc68242-at-[206.69.208.26]} } 20, 11 -- Date: Thu, 31 May 2007 15:08:51 -0500 } 20, 11 -- To: microscopy-at-microscopy.com } 20, 11 -- From: mdienelt-at-msn.com (by way of MicroscopyListserver) } 20, 11 -- Subject: viaWWW: Advice on critical point dryers } 20, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
-- Michael F. Dufraine EM - Product Manager TEL 800-992-9037 X340 MDufraine-at-ebsciences.com www.ebsciences.com
==============================Original Headers============================== 9, 26 -- From mdufraine-at-ebsciences.com Fri Jun 1 11:21:41 2007 9, 26 -- Received: from mx-outbound01.easydns.com (mailout.easydns.com [205.210.42.54]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51GLfPS028088 9, 26 -- for {microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 11:21:41 -0500 9, 26 -- Received: from mail.ebsciences.com (unknown [69.182.224.2]) 9, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 9, 26 -- (No client certificate requested) 9, 26 -- by mx-outbound01.easydns.com (Postfix) with ESMTP id A1D3D7FF6; 9, 26 -- Fri, 1 Jun 2007 12:21:39 -0400 (EDT) 9, 26 -- Received: from mdufraine.ebsciences.private ([10.10.0.196]) 9, 26 -- by mail.ebsciences.com with esmtpsa (SSLv3:AES256-SHA:256) 9, 26 -- (Exim 4.67) 9, 26 -- (envelope-from {mdufraine-at-ebsciences.com} ) 9, 26 -- id 1Hu9sh-0004kz-16; Fri, 01 Jun 2007 12:21:39 -0400 9, 26 -- Message-ID: {46604792.4060203-at-ebsciences.com} 9, 26 -- Date: Fri, 01 Jun 2007 12:21:38 -0400 9, 26 -- From: Mike Dufraine {mdufraine-at-ebsciences.com} 9, 26 -- Organization: Energy Beam Sciences 9, 26 -- User-Agent: Thunderbird 2.0.0.0 (Windows/20070326) 9, 26 -- MIME-Version: 1.0 9, 26 -- To: mdienelt-at-msn.com, microscopy-at-microscopy.com 9, 26 -- Subject: Re: [Microscopy] viaWWW: Advice on critical point dryers 9, 26 -- References: {200705312011.l4VKBHYH028552-at-ns.microscopy.com} 9, 26 -- In-Reply-To: {200705312011.l4VKBHYH028552-at-ns.microscopy.com} 9, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
No problem but you bring up a good point. I sent a posting and it didn't show up on the list server. A recent one involved a person in IL. I sent him a direct posting and a dseparate email posting to the list server. Nothing bounced back from either email but the later posting never made it to the list.
I fact, I see replies to postings before I ever see the original posting. I know things get delayed and that delays happen. It's not Nestor's fault. 'It's a server time slicing or sharing thing', IMO. In the next day or two, I might get the original posting. I have no problem there. My problem is that sometimes I never get or will ever receive the original posting (even my own).
So the other day after my posting failed, I sent a short posting to test my ability to still post to the list. I relied to: Re: [Microscopy] Re: AskAMicroscopist: Image Pro Analysis Question - URL with an Image and micrscopy-at-microscopy.com. My reply was posted and my posting received a reply from Mike Bode.
After a week and a half, my first posting still has never showed up.
I can see why some postings come through in double postings. I figure members know this 'lack of posting' problem and so they send double postings.
Paul
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I apologize if I was supposed to post this response to the list. Sometimes I don't see if a message comes from the group or an individual...
dj
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Email: info-at-nomadmet.com Name: Tom Doggart
Organization: Nomad Metallurgy, Inc
Title-Subject: [Filtered] Image Analysis software
Question: I am looking for a user friendly image analysis package to use with my Spot and Nikon cameras and/or their JPEG files. I would like it to do the following at a minimum:
A) Various linear and area measurement B) Phase % calculations for at least 3 phases in a structure C) Image Montague or stitching D) Expanded focus capabilities
Do you have any experience with any system that can do this?
Regardless of which software package you go with, my recommendation for your first step is to save your images as TIFFs!
At 05:47 PM 06/01/07, you wrote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
I use analySIS Opti s/w with Prior E103 Z control to do slice stacking. I don't know if Opti will control your cameras. I use Pixera 600CL and Olympus DP-70 (same driver). Opti controls the Prior stage.
There are several stitching programs. They all have plusses and minusses.
gary g.
At 02:47 PM 6/1/2007, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Fri Jun 1 17:55:26 2007 10, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l51MtQbB027531 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 17:55:26 -0500 10, 20 -- Message-Id: {200706012255.l51MtQbB027531-at-ns.microscopy.com} 10, 20 -- Received: (qmail 10828 invoked from network); 1 Jun 2007 15:55:25 -0700 10, 20 -- Received: by simscan 1.1.0 ppid: 10825, pid: 10826, t: 0.0947s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp4 with SMTP; 1 Jun 2007 15:55:25 -0700 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Fri, 01 Jun 2007 15:55:16 -0800 10, 20 -- To: info-at-nomadmet.com 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] viaWWW: looking for Image Analysis software 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200706012247.l51Mlrs5007769-at-ns.microscopy.com} 10, 20 -- References: {200706012247.l51Mlrs5007769-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-79CE6944 ==============================End of - Headers==============================
-- Shawn Mikula, Ph.D., Postdoctoral Scholar Center for Neuroscience University of California-Davis, 1544 Newton Court, Davis, CA 95618, Phone: 530-754-9209 Fax: 530-754-9136 mail: samikula-at-ucdavis.edu web: http://brainmaps.org
----- Original Message ----- X-from: {info-at-nomadmet.com} To: {samikula-at-ucdavis.edu} Sent: Friday, June 01, 2007 3:53 PM
I have used VIS by Visiopharm. It has incredible segmentation capabilities that are attached to a database. You can build essentially a program to separate an image into the regions of interest and then to all sorts of calculations. This is all done by simple clicking.
----- Original Message ----- X-from: {info-at-nomadmet.com} To: {rboehrin-at-vt.edu} Sent: Friday, June 01, 2007 6:53 PM
Tom
Definitely do not use JPEG. Why spend the money on a quality microscope and quality camera, and then save the image in a bad file-format?
For "expanded (sic extended) focus", you should consider using CombineZ5 (or ZM) and/or Helicon Focus. Each has excellent support and cost.
I would also plug ImageJ and the excellent support/cost.
regards,
Jim
} From mail-at-ns.microscopy.com Fri Jun 1 18:44:41 2007 } Date: Fri, 1 Jun 2007 17:46:48 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: info-at-nomadmet.com } Reply-to: info-at-nomadmet.com } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] viaWWW: looking for Image Analysis software } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both info-at-nomadmet.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: info-at-nomadmet.com } Name: Tom Doggart } } Organization: Nomad Metallurgy, Inc } } Title-Subject: [Filtered] Image Analysis software } } Question: I am looking for a user friendly image analysis package to use with my Spot and Nikon cameras and/or their JPEG files. I would like it to do the following at a minimum: } } } } A) Various linear and area measurement } B) Phase % calculations for at least 3 phases in a structure } C) Image Montague or stitching } D) Expanded focus capabilities } } } } Do you have any experience with any system that can do this? } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 11 -- From zaluzec-at-microscopy.com Fri Jun 1 17:45:11 2007 } 11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51MjA7f004949 } 11, 11 -- for {microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 17:45:10 -0500 } 11, 11 -- Mime-Version: 1.0 } 11, 11 -- Message-Id: {p06240800c28651b9d48b-at-[206.69.208.22]} } 11, 11 -- Date: Fri, 1 Jun 2007 17:45:09 -0500 } 11, 11 -- To: microscopy-at-microscopy.com } 11, 11 -- From: info-at-nomadmet.com (by way of MicroscopyListserver) } 11, 11 -- Subject: viaWWW: looking for Image Analysis software } 11, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 12 -- From jquinn-at-www.matscieng.sunysb.edu Sat Jun 2 11:51:44 2007 9, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 9, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l52GpiPe017688 9, 12 -- for {microscopy-at-microscopy.com} ; Sat, 2 Jun 2007 11:51:44 -0500 9, 12 -- Received: (from jquinn-at-localhost) 9, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id l52GnOh19572; 9, 12 -- Sat, 2 Jun 2007 12:49:24 -0400 9, 12 -- Date: Sat, 2 Jun 2007 12:49:24 -0400 9, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 9, 12 -- Message-Id: {200706021649.l52GnOh19572-at-www.matscieng.sunysb.edu} 9, 12 -- To: info-at-nomadmet.com, microscopy-at-microscopy.com 9, 12 -- Subject: Re: [Microscopy] viaWWW: looking for Image Analysis software ==============================End of - Headers==============================
If the DNA is already attached to a substrate, I'd really suggest that you try AFM instead. It will give you essentially atomic level resolution, without any disruptive coating.
Contact me off line if you need more info.
Thanks Barbara
At 12:12 AM 6/4/2007, dac-at-research.umass.edu wrote:
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==============================Original Headers============================== 11, 20 -- From bfoster-at-mme1.com Mon Jun 4 00:17:14 2007 11, 20 -- Received: from smtp109.sbc.mail.re2.yahoo.com (smtp109.sbc.mail.re2.yahoo.com [68.142.229.96]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l545HDLO017834 11, 20 -- for {microscopy-at-microscopy.com} ; Mon, 4 Jun 2007 00:17:14 -0500 11, 20 -- Message-Id: {200706040517.l545HDLO017834-at-ns.microscopy.com} 11, 20 -- Received: (qmail 95349 invoked from network); 4 Jun 2007 05:17:13 -0000 11, 20 -- Received: from unknown (HELO barbsd505.mme1.com) (bfostermme-at-sbcglobal.net-at-68.94.37.215 with login) 11, 20 -- by smtp109.sbc.mail.re2.yahoo.com with SMTP; 4 Jun 2007 05:17:13 -0000 11, 20 -- X-YMail-OSG: PpL5dW0VM1kt8KYnIVL4g1oZMUp4.mahfGdZvNfji8X3zsfIuVsrQrnVOiJq9Kv9LV7U_YndJVnWx5tToDmY496aw_dkSDJumIHrq99QK17oZO2lsNRydtQNUdeEZW3hzCHYDc5ggnkDLuyycuDuyIwY17l8iDrk53o2k_OQFOY- 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Mon, 04 Jun 2007 00:16:37 -0500 11, 20 -- To: dac-at-research.umass.edu, microscopy-at-microscopy.com 11, 20 -- From: Barbara Foster {bfoster-at-mme1.com} 11, 20 -- Subject: Re: [Microscopy] Re: viaWWW: Metal Shadow Casting of DNA 11, 20 -- In-Reply-To: {200706011457.l51Evc6o005808-at-ns.microscopy.com} 11, 20 -- References: {200706011457.l51Evc6o005808-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 11, 20 -- Content-Transfer-Encoding: 8bit 11, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l545HDLO017834 ==============================End of - Headers==============================
Dale Callaham wrote: ================================================================== } I don't know the particulars of operation of the evaporator unit and my } knowledge is for pure gold or platinum (some metals will alloy with the } tungsten and cause problems - maybe someone else knows). } } Platinum MP = 1772 C } Tungsten MP = 3410 C } } If the Pt wire is not melting, then the tungsten temperature is too low. } If you advance the filament heating slowly (a few seconds for work, but } slower in testing until you know the behaviour) you should first see the } Pt:Pd wire melt and flow to the bottom of the hairpin (the point of the } V is down) and the droplet will evaporate at a slightly higher } temperature. Actually you only need to put the wire near the tip of the V. } } Just remember to heat the filament over a few seconds, not instantly. } Tungsten has a positive temperature coefficient and the filament } resistance is initially low and a large surge will occur if full voltage } is applied directly - it can cause the evaporant wire to spit or the } filament to burn out; but you shouldn't need to be that close to the } tungten melting point. } } You don't specify distances but it sounds like you are using a lot of } wire. You probably only need 10-20Å. 0.5 cm of 8 mil wire at 10 cm } distance will give ~13 Å (at normal incidence, or the sides of DNA so } disposed....) } } Å = (40.3 * diameter^2 * Length) / distance^2 } } diameter in "mils" (0.001"), 8 mils is a standard size, 0.008" dia } length of wire evaporant, distance to sample in cm ================================================================= I have always thought that the best way to "shadow" was to use the "Pt/C sesile drop" method. I learned about it many years ago in graduate school and don't know who used it originally. But one gets a finer grain if Pt/C is evaporated simultaneously than if Pt alone is evaporated. The way to do this is to take a carbon rod that has been sharpened down to a "neck", wrap about the "neck" not more than 30-40 mm of 8 mil Pt wire, put the bell jar onto the vacuum evaporator but don't pump down. Slowly increase the current through the carbon rods to the point where, just beyond cherry red, the Pt melts and because of surface tension effects, and the fact that liquid Pt does not want to wet carbon, it "beads up", just as water does on a freshly waxed car. Once this happens, turn off the current, and when it cools down, rotate the bead so that it is facing the surface to be shadowed.
Then the bell jar is put in place, the chamber pumped down, and the carbon rod is evaporated but what really comes off is a combination of Pt and C.
Note: The optimum amount of wire to be used depends on a)distance to the substrate to be coated and b) shadowing angle.
Since Pt wants to alloy readily with W, this approach avoids all those other issues as well.
Disclaimer: SPI Supplies offers on our website Pt wire and presharpened carbon rods so we would have a vested interest in seeing this technique used more rather than less frequently.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
==============================Original Headers============================== 9, 20 -- From cgarber-at-2spi.com Mon Jun 4 00:59:12 2007 9, 20 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l545xCIY029855 9, 20 -- for {microscopy-at-msa.microscopy.com} ; Mon, 4 Jun 2007 00:59:12 -0500 9, 20 -- Received: from [192.168.1.101] (c-71-230-36-118.hsd1.pa.comcast.net [71.230.36.118]) 9, 20 -- (authenticated bits=0) 9, 20 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id l545xBSh019678 9, 20 -- for {microscopy-at-msa.microscopy.com} ; Mon, 4 Jun 2007 01:59:12 -0400 9, 20 -- X-IDV-FirstRcvd: c-71-230-36-118.hsd1.pa.comcast.net [71.230.36.118] 9, 20 -- X-IDV-HELO: [192.168.1.101] 9, 20 -- X-IDV-Authenticated-User: cgarber 9, 20 -- Message-ID: {4663AA2F.7060406-at-2spi.com} 9, 20 -- Date: Mon, 04 Jun 2007 01:59:11 -0400 9, 20 -- From: "Garber, Charles A" {cgarber-at-2spi.com} 9, 20 -- User-Agent: Thunderbird 2.0.0.0 (Windows/20070326) 9, 20 -- MIME-Version: 1.0 9, 20 -- To: Microscopy Listserver {microscopy-at-msa.microscopy.com} 9, 20 -- Subject: Shadowing with Pt for TEM 9, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I second Barbara Foster's suggestion. For an example of DNA imaging by AFM, see our website: http://www.asmicro.com/Applications/DNA_Molecules.htm
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] ============================================= ----- Original Message ----- From: bfoster-at-mme1.com To: donc-at-asmicro.com Sent: Monday, June 04, 2007 1:21 AM Subject: [a] [Microscopy] viaWWW: Metal Shadow Casting of DNA
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Sharon,
If the DNA is already attached to a substrate, I'd really suggest that you try AFM instead. It will give you essentially atomic level resolution, without any disruptive coating.
Contact me off line if you need more info.
Thanks Barbara
At 12:12 AM 6/4/2007, dac-at-research.umass.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Shannon, } } I don't know the particulars of operation of the evaporator unit and my } knowledge is for pure gold or platinum (some metals will alloy with the } tungsten and cause problems - maybe someone else knows). } } Platinum MP = 1772 C } Tungsten MP = 3410 C } } If the Pt wire is not melting, then the tungsten temperature is too low. } If you advance the filament heating slowly (a few seconds for work, but } slower in testing until you know the behaviour) you should first see the } Pt:Pd wire melt and flow to the bottom of the hairpin (the point of the } V is down) and the droplet will evaporate at a slightly higher } temperature. Actually you only need to put the wire near the tip of the V. } } Just remember to heat the filament over a few seconds, not instantly. } Tungsten has a positive temperature coefficient and the filament } resistance is initially low and a large surge will occur if full voltage } is applied directly - it can cause the evaporant wire to spit or the } filament to burn out; but you shouldn't need to be that close to the } tungten melting point. } } You don't specify distances but it sounds like you are using a lot of } wire. You probably only need 10-20Å. 0.5 cm of 8 mil wire at 10 cm } distance will give ~13 Å (at normal incidence, or the sides of DNA so } disposed....) } } Å = (40.3 * diameter^2 * Length) / distance^2 } } diameter in "mils" (0.001"), 8 mils is a standard size, 0.008" dia } length of wire evaporant, distance to sample in cm } } I have an Excel spreadsheet with a "calculator" for this formula that I } can send if you want - can't go to the Microscopy Listserver..... Just ask. } } Hope this helps. } } Dale Callaham } Univ. of Massachusetts, Amherst } } } modla-at-dbi.udel.edu wrote: } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at http://ns.microscopy.com/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from } a Subscriber, so when replying } } please copy both modla-at-dbi.udel.edu as well } as the MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: modla-at-dbi.udel.edu } } Name: Shannon Modla } } } } Organization: Delaware Biotechnology Institute } } } } Title-Subject: [Filtered] Metal Shadow Casting of DNA } } } } Question: We are currently trying to rotary } shadow cast DNA but are having problems } obtaining consistent results with the metal shadowing. We are using an } 80:20 } platinum-palladium wire purchased from EMS and a Denton Bench Top Turbo } III sputter coater. } The platinum palladium wire was wrapped around a 2-3 cm portion of a } hairpin loop formed } with tungsten wire. When the filament power is increased, the platinum } palladium wire will } either heat up without melting and evaporating or else the tungsten wire } breaks without } appreciable metal shadowing. I was wondering if anyone had any } experience with } this technique and could offer suggestions about how to obtain more } consistent results. } } } } Thanks, } } } } Shannon Modla } } Delware Biotechnology Institute } } BioImaging Center } } } } } } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== ... } } 9, 11 -- Date: Fri, 1 Jun 2007 07:50:12 -0500 } } 9, 11 -- To: microscopy-at-microscopy.com } } 9, 11 -- From: modla-at-dbi.udel.edu (by way of MicroscopyListserver) } } 9, 11 -- Subject: viaWWW: Metal Shadow Casting of DNA } } 9, 11 -- Content-Type: text/plain; charset="us-ascii" } } ==============================End of - } Headers============================== ... } 14, 21 -- From dac-at-research.umass.edu Fri Jun 1 09:55:15 2007 } 14, 21 -- Received: from race4.oit.umass.edu } (race4.oit.umass.edu [128.119.101.40]) } 14, 21 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id l51EtEr4002073 } 14, 21 -- for {Microscopy-at-microscopy.com} ; } Fri, 1 Jun 2007 09:55:15 -0500
==============================Original Headers============================== 17, 24 -- From donc-at-asmicro.com Mon Jun 4 08:29:44 2007 17, 24 -- Received: from smtp111.sbc.mail.re2.yahoo.com (smtp111.sbc.mail.re2.yahoo.com [68.142.229.94]) 17, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l54DThYA018354 17, 24 -- for {microscopy-at-microscopy.com} ; Mon, 4 Jun 2007 08:29:43 -0500 17, 24 -- Received: (qmail 37684 invoked from network); 4 Jun 2007 13:29:43 -0000 17, 24 -- Received: from unknown (HELO asm15) (asmicro-at-sbcglobal.net-at-68.23.9.238 with login) 17, 24 -- by smtp111.sbc.mail.re2.yahoo.com with SMTP; 4 Jun 2007 13:29:42 -0000 17, 24 -- X-YMail-OSG: wxcJFFoVM1nYRj0FMOh83REceMi8qLEX6NzzIJNqiB1Zrg5FCx4MaG0i5XzsoOmR8Khn9DEZ5_g.l5U1wmjsRbBh_GGZ57_XVKpUgtMYNRCeM10OVvOpYixngy8IiOceIe7n0hIJDI6JEKqEKC65krTscifmimglTYUwNgcexDwQ 17, 24 -- Message-ID: {001601c7a6ac$6858e330$6901a8c0-at-asm15} 17, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 17, 24 -- To: {bfoster-at-mme1.com} , "Microscopy List" {microscopy-at-microscopy.com} 17, 24 -- References: {200706040521.l545LZE3022955-at-ns.microscopy.com} 17, 24 -- Subject: Re: [a] [Microscopy] viaWWW: Metal Shadow Casting of DNA 17, 24 -- Date: Mon, 4 Jun 2007 09:29:28 -0400 17, 24 -- MIME-Version: 1.0 17, 24 -- Content-Type: text/plain; 17, 24 -- format=flowed; 17, 24 -- charset="iso-8859-1"; 17, 24 -- reply-type=original 17, 24 -- Content-Transfer-Encoding: 8bit 17, 24 -- X-Priority: 3 17, 24 -- X-MSMail-Priority: Normal 17, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3028 17, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 ==============================End of - Headers==============================
I'm reading Falk, Brill and Stork's "Seeing the Light: Optics in Nature, Photography, Color, Vision, and Holography." I like the book, in fact love it, but I want something more dedicated to the physics/math. I did order the Schaum's outline for problems.
Can someone recommend a good undergraduate level optics text? My college level math and physics are fine.
Has anyone used Eugene Hecht's Optics either as a student or instructor for a course? Is it good enough to actually buy, or are there better texts?
Thanks,
K. Leo Pullin
==============================Original Headers============================== 1, 19 -- From kleopullin-at-pacbell.net Mon Jun 4 14:48:56 2007 1, 19 -- Received: from web83411.mail.sp1.yahoo.com (web83411.mail.sp1.yahoo.com [69.147.64.59]) 1, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l54JmunN008288 1, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 4 Jun 2007 14:48:56 -0500 1, 19 -- Received: (qmail 89759 invoked by uid 60001); 4 Jun 2007 19:26:11 -0000 1, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 1, 19 -- s=s1024; d=pacbell.net; 1, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 1, 19 -- b=0vM1D/7vc57qgV0I82v2GjAnaog0bpzR064ixJcjfMRp+odYURE3SWEI40ed4D6dSaMwww+0zmTSYocX+ioapkWXoTXjXwpqIiX2fAUboN8J0U5n+bCtSufgihHWet5997UjlnyX3PMXCrhchvwy007ckDHqanz5/7LMBdeCPIs=; 1, 19 -- X-YMail-OSG: oeCbtN0VM1nO4bG5RWWk8gesgyEkUmyg83tp5cPuAe6Fw.uV 1, 19 -- Received: from [69.226.100.188] by web83411.mail.sp1.yahoo.com via HTTP; Mon, 04 Jun 2007 12:26:11 PDT 1, 19 -- Date: Mon, 4 Jun 2007 12:26:11 -0700 (PDT) 1, 19 -- From: Kleo Pullin {kleopullin-at-pacbell.net} 1, 19 -- Subject: LM Optical physics text -- recommendations? 1, 19 -- To: Microscopy-at-microscopy.com 1, 19 -- MIME-Version: 1.0 1, 19 -- Content-Type: text/plain; charset=iso-8859-1 1, 19 -- Content-Transfer-Encoding: 8bit 1, 19 -- Message-ID: {670379.83959.qm-at-web83411.mail.sp1.yahoo.com} ==============================End of - Headers==============================
ImageJ is free and you get what you paid for. I do not know whether it will control the Prior stage controllers. If it does, great. Problem solved.
However, if not, then you need industrial strength software like analySIS--or current generation. This controls the Z axis stepper and automatically collects Z stacks and then assembles them into a high depth of focus final image. I typically do 20 slices. OK....do this yourself manually and try to get a good result. I did and after beating my head against the wall too many times, it was obvious that another method was necessary.
The focus assembly method is based on contrast. So many small increments of Z are ideal. analySIS and the Prior stage controller are seamlessly integrated.
gary g.
At 03:04 PM 6/1/2007, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 10, 21 -- From gary-at-gaugler.com Mon Jun 4 15:25:10 2007 10, 21 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l54KPASn020921 10, 21 -- for {microscopy-at-microscopy.com} ; Mon, 4 Jun 2007 15:25:10 -0500 10, 21 -- Message-Id: {200706042025.l54KPASn020921-at-ns.microscopy.com} 10, 21 -- Received: (qmail 29863 invoked from network); 4 Jun 2007 13:25:09 -0700 10, 21 -- Received: by simscan 1.1.0 ppid: 29860, pid: 29861, t: 0.1494s 10, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 21 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 21 -- by qsmtp1 with SMTP; 4 Jun 2007 13:25:09 -0700 10, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 21 -- Date: Mon, 04 Jun 2007 13:24:10 -0800 10, 21 -- To: samikula-at-ucdavis.edu 10, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 21 -- Subject: Re: [Microscopy] Re: viaWWW: looking for Image Analysis 10, 21 -- software 10, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 21 -- In-Reply-To: {200706012304.l51N4xbP009673-at-ns.microscopy.com} 10, 21 -- References: {200706012304.l51N4xbP009673-at-ns.microscopy.com} 10, 21 -- Mime-Version: 1.0 10, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-7C5A6774 ==============================End of - Headers==============================
Is there anybody here who has used the SPI Combined Sputter and Carbon Coater System? Any feedback? For those who have combined systems, have you encountered any contamination issues? How can this best be avoided?
Aside from good sputtering/coating capability, we are also considering having a dual system with smaller footprint since we have a very crowded lab. Any brand/model you can suggest?
Thank you so much.
Melina Miralles Lab Technician The Petroleum Institute Abu Dhabi, UAE
==============================Original Headers============================== 7, 27 -- From mmiralles-at-pi.ac.ae Tue Jun 5 00:06:07 2007 7, 27 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l555651V010495 7, 27 -- for {microscopy-at-microscopy.com} ; Tue, 5 Jun 2007 00:06:07 -0500 7, 27 -- Received: from pi-exf.pi.ac.ae ([192.168.2.11]) 7, 27 -- by mx1.pi.ac.ae with ESMTP; 05 Jun 2007 09:10:17 +0400 7, 27 -- X-IronPort-AV: i="4.16,383,1175457600"; 7, 27 -- d="scan'208"; a="1648798:sNHT24529136" 7, 27 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.1830); 7, 27 -- Tue, 5 Jun 2007 09:05:58 +0400 7, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 27 -- Content-class: urn:content-classes:message 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="windows-1256" 7, 27 -- Subject: Sputter & Carbon Coater 7, 27 -- Date: Tue, 5 Jun 2007 09:05:56 +0400 7, 27 -- Message-ID: {D5603421C6303A46883F87E7968F285B02DFEA31-at-pi-exm.PI.AC.AE} 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- Thread-Topic: Sputter & Carbon Coater 7, 27 -- Thread-Index: AcenLzLfFcyEFZDRQJiG1jBHFpDM3Q== 7, 27 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae} 7, 27 -- To: {microscopy-at-microscopy.com} 7, 27 -- X-OriginalArrivalTime: 05 Jun 2007 05:05:58.0273 (UTC) FILETIME=[34196710:01C7A72F] 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l555651V010495 ==============================End of - Headers==============================
Dr Peters gives me permission today by email to quote on the LisServer her clarification/correction, sent to me earlier today, that only UrAc, but not Pb Citrate, was used as a block stain in her work. Personally, I am quite grateful we were briefly misled, because it brought in David Elliott's response on his use of lead acetate block stain (in EtOH-acetone 50:50) following aqueous UrAc.
Here is Dr Peter's clarification/correction: "Our method is as follows: We use uranyl acetate in 70% ethanol as a block stain ....... then [after] the tissue is embedded in Epon and polymerized..... We use the lead citrate only for the ready prepared sections. I am sorry if my phrasing [turned out to] be kind of misleading."
I plan continued exploration of both lead salts. Use as a block stain in organic solvent after UrAc especially interests me for freeze-substitution. So far, I find both are insoluble or nearly so in 100% acetone, so the EtOH-containing vehicle in Elliott's procedure seems necessary. Does anyone know or remember if the extreme alkalinity of aqueous lead citrate section stains is essential for releasing the Pb from the strongly sequestering citrate? I'm not sure that pH 11 can be approached in pure organic solvent, but why not try....?
I would guess that prolonging the exposure of tissue blocks to organic solvent for extended block-staining is NOT likely to cause additional shrinkage or extraction of cells, because I think the binding of metals tends to stabilizes the components. ---not completely against shrinkage however; I find that freeze-substitution in acetone-TA followed by acetone UrAc fails to prevent variable amounts of shrinkage, 5-12%, of the myofilament lattice spacing in striated muscle; I'd like someday to see the process monitored by x-ray cryo-diffraction to identify the stage where this occurs and seek ways to prevent or minimize the shrinkage.
-mike reedy-
*************************** At 10:07 PM -0500 5/27/07, dianavd-at-eye.usyd.edu.au wrote: ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Geoff McA asked for the reference to the paper I mentioned. It's Peters S et al; American Journal of Ophthalmology (2007) 143:995-1002; Ultrastructural findings in the primate eye after intravitreal injection of Bevacizumab. All it says is
"postfixed with 1% OsO4 at room temperature in 0.1 M cacodylate buffer (pH 7.4) for three hours, bloc-stained with uranyl acetate and lead citrate, and embedded in Epon after dehydration in a graded series of acetones"
I've Emailed asking for more details. I have the PDF if anyone would like to see the pics.
Diana
} ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } I've just read a paper where the author talks about block staining } with uranyl acetate and lead citrate. UAc yes, but Pbcitrate? Has } anyone heard of this? Does it work? The pictures in the paper were } very nice; good contrast and detail. With the recent talk about } general lack of contrast in specimens these days (I quite agree on } that; I find contrast poorer now than in the past), could this be } a new method? } } All opinions please! } } Cheers, } } Diana }
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
For those of you following the use of lead citrate as a block stain discussion, I had a reply from the author of the paper where the method was described. It turns out to have been incorrect; PbC was not used as a block stain. It was an English usage problem - the author is German - she actually used the PbC in the usual way as a section stain. Still, the idea got us here on the List thinking!
Cheers,
Diana
==============================Original Headers============================== 4, 18 -- From dianavd-at-eye.usyd.edu.au Tue Jun 5 19:23:45 2007 4, 18 -- Received: from galen.med.usyd.edu.au (nugalen.med.usyd.edu.au [129.78.36.39]) 4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l560NiMh031539 4, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 5 Jun 2007 19:23:45 -0500 4, 18 -- Received: from [129.78.203.144] (helo=[129.78.203.144]) 4, 18 -- by galen.med.usyd.edu.au with esmtp (Exim 4.43) 4, 18 -- id 1HvjJO-0006Mg-QC 4, 18 -- for Microscopy-at-microscopy.com; Wed, 06 Jun 2007 10:23:42 +1000 4, 18 -- Mime-Version: 1.0 (Apple Message framework v752.2) 4, 18 -- Content-Transfer-Encoding: 7bit 4, 18 -- Message-Id: {0CF1515D-7F8A-490C-B62F-2FE8CFF0C98F-at-eye.usyd.edu.au} 4, 18 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 4, 18 -- To: Microscopy {Microscopy-at-microscopy.com} 4, 18 -- From: Diana van Driel {dianavd-at-eye.usyd.edu.au} 4, 18 -- Subject: Pb citrate block staining - followup 4, 18 -- Date: Wed, 6 Jun 2007 10:23:37 +1000 4, 18 -- X-Mailer: Apple Mail (2.752.2) 4, 18 -- X-Spam-Score: -4.4 (----) ==============================End of - Headers==============================
Hi - We have an old Electroscan which has proved to be fairly reliable over the years, but lately it has been having problems. Recently, the CPU board died and we were fortunate enough to have a spare (we basically have a spare for everything with this machine). This spare worked in that I could communicate with the CPU via its RS232 port and have full access to its command set. With the self test the CPU board says its OK. Unfortunately, we now seem to have no image coming through. I've played with the command set menu hoping to get an image. At one point I was able to see an extremely weak signal but I'm not sure how I really achieved it simply because there are so many different command options. What I want is Volume 6 - APPENDICES, from the ElectroScan manual set (we have the others). This manual goes into depth about the system software structure. What I also want, if possible, is the service-engineer manual which would detail the actual initial setup of the E3, and any advice in regard to my E3 problem is really welcome.
Thanks
Peter Duncan Senior Technician
==============================Original Headers============================== 4, 30 -- From pcduncan-at-cyllene.uwa.edu.au Tue Jun 5 20:41:03 2007 4, 30 -- Received: from asclepius2.uwa.edu.au (asclepius2.uwa.edu.au [130.95.128.59]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l561f2c1011618 4, 30 -- for {Microscopy-at-Microscopy.Com} ; Tue, 5 Jun 2007 20:41:03 -0500 4, 30 -- Received: from panacea.kas (localhost.localdomain [127.0.0.1]) 4, 30 -- by panacea.uwa.edu.au (Postfix) with SMTP id CAC4988A14 4, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 6 Jun 2007 09:41:01 +0800 (WST) 4, 30 -- Received: from panacea (localhost.localdomain [127.0.0.1]) 4, 30 -- by panacea.prekas (Postfix) with ESMTP id 53DFF88961 4, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 6 Jun 2007 09:41:01 +0800 (WST) 4, 30 -- X-UWA-Client-IP: 130.95.124.134 (UWA) 4, 30 -- Received: from [130.95.124.134] (tearm.cmm.uwa.edu.au [130.95.124.134]) 4, 30 -- by panacea.input (Postfix) with ESMTP id A5EA4889F8 4, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 6 Jun 2007 09:41:00 +0800 (WST) 4, 30 -- Mime-Version: 1.0 (Apple Message framework v624) 4, 30 -- Content-Transfer-Encoding: 7bit 4, 30 -- Message-Id: {1ecd241b5a6eb5723598b05d0f41da01-at-cyllene.uwa.edu.au} 4, 30 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 30 -- To: Microscopy-at-Microscopy.Com 4, 30 -- From: Peter Duncan {pcduncan-at-cyllene.uwa.edu.au} 4, 30 -- Subject: SEM - Electroscan E3 problem - require service engineer manual for initial setup procedure. 4, 30 -- Date: Wed, 6 Jun 2007 09:43:37 +0800 4, 30 -- X-Mailer: Apple Mail (2.624) 4, 30 -- X-Anti-Virus: Kaspersky Anti-Virus for MailServers 5.5.10/RELEASE, bases: 06062007 #318960, status: clean 4, 30 -- X-SpamTest-Info: Profile: Formal (1243/070604) 4, 30 -- X-SpamTest-Info: Profile: Detect Hard [UCS 2006-10-25] 4, 30 -- X-SpamTest-Info: Profile: SysLog 4, 30 -- X-SpamTest-Info: Profile: Marking Spam - Subject (UCS) [2006-10-25] 4, 30 -- X-SpamTest-Status: Not detected 4, 30 -- X-SpamTest-Version: SMTP-Filter Version 2.0.0 [0125], KAS/Release ==============================End of - Headers==============================
Some days ago there was a question posed about the educational benefits of table top SEM and nano-scale samples. In a few weeks I'll post my experiences using a Hitachi TM-1000 table top SEM in a Nanotechnology course for high schoolers as part of the Duke University Talent Identification Program (TIP). More about the program can be found at 'www.tip.duke.edu'. I've chosen the TM-1000 to introduce the students to the micro and nanoscale, and we will be using the SEM, in addition to AFM and HRTEM to characterize Au and Ag nanoparticles the students synthesize as part of a experiential learning activity.
In my opinion the table top SEM will allow the students hands-on experience with an electron microscope. Not that it will resolve atomic columns in nanoparticles, but as a way to introduce them to the scale of things. The intention is to let the students choose ANY samples they are interested in and let them discover the detail of features on the micro and nanoscale.
If anyone has had experience integrating these microscopies into a high school course on nanotechnology I would be most interested in learning more about how it was accomplished and the outcomes. I would also be more than happy to share the syllabus created for the course if requested.
Stay tuned, I'll post the student's data and feedback on the table top SEM, AFM and TEM.
Donovan
==============================Original Headers============================== 13, 28 -- From donovan.leonard-at-gmail.com Tue Jun 5 22:13:59 2007 13, 28 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.226]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l563DxNI024420 13, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 5 Jun 2007 22:13:59 -0500 13, 28 -- Received: by wx-out-0506.google.com with SMTP id s14so7628wxc 13, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 05 Jun 2007 20:13:58 -0700 (PDT) 13, 28 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 13, 28 -- d=gmail.com; s=beta; 13, 28 -- h=domainkey-signature:received:received:message-id:date:from:user-agent:mime-version:to:subject:content-type:content-transfer-encoding; 13, 28 -- b=dyfVrH1XBQkmwdsDufL4zkQVNizVyorQxOguKO1Y1BAksaT8760tGnsprMsuG43dTxEh3irILCXBg/kzxQj4AKaZKQMhTcqENG4Rkm7waREJgOgZE1SKysd+D2gmkenYL+uUcRAm+xdcfdmCiNHq2v5NB24SRXOqyRLK8i9jhoM= 13, 28 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 13, 28 -- d=gmail.com; s=beta; 13, 28 -- h=received:message-id:date:from:user-agent:mime-version:to:subject:content-type:content-transfer-encoding; 13, 28 -- b=FEhNU7orpaaLyXrz/KH/TSVRGLUGh1JI85MEocNjmPB0aOMRisv7LjWEXjIXXMdgX/L/3+Pi5wBcFMvmawex38VOjSHhS1XTV4LB/1/cDxrmbC0gLfENbDR3RdgvogTDZ+DWU2atIamfmAktj2IMTYN+z5bx9APD/RVY3RUTMvY= 13, 28 -- Received: by 10.70.87.5 with SMTP id k5mr113569wxb.1181099638766; 13, 28 -- Tue, 05 Jun 2007 20:13:58 -0700 (PDT) 13, 28 -- Received: from ?192.168.1.95? ( [216.77.224.100]) 13, 28 -- by mx.google.com with ESMTP id i37sm3549106wxd.2007.06.05.20.13.57; 13, 28 -- Tue, 05 Jun 2007 20:13:57 -0700 (PDT) 13, 28 -- Message-ID: {4666266E.5060904-at-gmail.com} 13, 28 -- Date: Tue, 05 Jun 2007 23:13:50 -0400 13, 28 -- From: DNL {donovan.leonard-at-gmail.com} 13, 28 -- User-Agent: Thunderbird 1.5.0.12 (Windows/20070509) 13, 28 -- MIME-Version: 1.0 13, 28 -- To: Microscopy-at-microscopy.com 13, 28 -- Subject: Table Top SEM & High School Nano Course 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have helped get a SEM put into my local high school. The science teacher there now is able to offer 8 college credits to students that take his advanced program related to this instrument. Mark would be able to talk to you more about that aspect and how he integrates it into his curriculum. I have cc'd him with this email so you'll have his email address. This is still in the beginning stages so there is a lot yet to know and learn. But I think it is an absolutely fabulous opportunity for learning...
In the process of getting the SEM into my local high school, I have learned a fair amount about SEM usage in high schools in the US and a bit internationally. There are pockets of high schools around the USA that have this kind of technology operating in high schools, but not a lot. Germany has some high schools using it, apparently Japan has it extensively used throughout their high school system.
You are using Au and Ag particles. My experience indicates high school students seem more interested in biological samples rather than materials. It would be very useful if a materials curriculum could be developed that would interest students at this level.
Finding teachers and school systems that are open to this and have the science background to make it work well, I think is a large hurdle. If a solid curriculm could be put together that would work as a framework for teachers to use, I think that would be very helpful.
Schools are, justifiably, reluctant to use this technology as maintaining these machines is quite expensive and most schools run on very tight budgets. In the cases where I have seen SEM's working in high schools, there has usually been someone that is knowledgable about the instruments and can maintain them for the school pro-bono. These individuals have been key in getting SEM's into high schools. (There may be a program in western PA that's an exception to this, but I've not had any luck contacting them.)
Do send me your curriculum and further information about what you are doing. I can give you more details of programs I've discovered if you wish, but I don't think those details are of general interest to the list (do correct me if I'm wrong).
dj
On Tue, 5 Jun 2007, donovan.leonard-at-gmail.com wrote:
} Hello Listsers - } } Some days ago there was a question posed about the educational benefits } of table top SEM and nano-scale samples. In a few weeks I'll post my } experiences using a Hitachi TM-1000 table top SEM in a Nanotechnology } course for high schoolers as part of the Duke University Talent } Identification Program (TIP). More about the program can be found at } 'www.tip.duke.edu'. I've chosen the TM-1000 to introduce the students } to the micro and nanoscale, and we will be using the SEM, in addition to } AFM and HRTEM to characterize Au and Ag nanoparticles the students } synthesize as part of a experiential learning activity. } } In my opinion the table top SEM will allow the students hands-on } experience with an electron microscope. Not that it will resolve atomic } columns in nanoparticles, but as a way to introduce them to the scale of } things. The intention is to let the students choose ANY samples they } are interested in and let them discover the detail of features on the } micro and nanoscale. } } If anyone has had experience integrating these microscopies into a high } school course on nanotechnology I would be most interested in learning } more about how it was accomplished and the outcomes. I would also be } more than happy to share the syllabus created for the course if requested. } } Stay tuned, I'll post the student's data and feedback on the table top } SEM, AFM and TEM. } } Donovan
==============================Original Headers============================== 11, 19 -- From dljones-at-bestweb.net Wed Jun 6 08:07:58 2007 11, 19 -- Received: from vms044pub.verizon.net (vms044pub.verizon.net [206.46.252.44]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56D7wjs014386 11, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 08:07:58 -0500 11, 19 -- Received: from localhost ([71.247.229.59]) 11, 19 -- by vms044.mailsrvcs.net (Sun Java System Messaging Server 6.2-6.01 (built Apr 11, 19 -- 3 2006)) with ESMTPA id {0JJ7000MBTSK7117-at-vms044.mailsrvcs.net} for 11, 19 -- Microscopy-at-microscopy.com; Wed, 06 Jun 2007 08:07:44 -0500 (CDT) 11, 19 -- Date: Wed, 06 Jun 2007 09:08:42 -0400 (Eastern Daylight Time) 11, 19 -- From: "David L. Jones" {dljones-at-bestweb.net} 11, 19 -- Subject: Re: [Microscopy] Table Top SEM & High School Nano Course 11, 19 -- In-reply-to: {200706060317.l563H7XP030532-at-ns.microscopy.com} 11, 19 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 11, 19 -- To: donovan.leonard-at-gmail.com 11, 19 -- Cc: Microscopy-at-microscopy.com, Mark Patinella {mpatinel-at-haldane.lhric.org} 11, 19 -- Message-id: {Pine.WNT.4.64.0706060825240.3076-at-H-F1} 11, 19 -- MIME-version: 1.0 11, 19 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 11, 19 -- References: {200706060317.l563H7XP030532-at-ns.microscopy.com} ==============================End of - Headers==============================
We unexpectedly have about $150,000 - $180,000 to potentially spend on a new microscope/digital vision system, and we are trying to determine which type of system would give us more relevant information about our product's components. We want to examine the following materials: the tips of ball point, fountain and roller ball pens (iron, tungsten carbide, brass, nickel-coated brass, nickel silver alloys, ceramic), plastic tip holders and ink reservoirs (polyethylene/polypropylene, nylon, PET), absorbent materials of various sizes and porosities (cellulosic, natural or artificial fibers). We are also interested in examining high-concentration micronized pigment dispersions to determine particle size (approximately 1 micron), shape and/or particle size distribution.
We are trying to decide between a tabletop SEM (either the Hitachi TM-1000 or the FEI Phenom) or a scanning laser microscope (Keyence VK-9700). We have a very limited amount of space to work with - anything larger than a desktop unit would require part of the budget to be spent on building a new room - and that is virtually impossible to get approved. Practically speaking, it's a desktop unit or nothing.
So, my questions are: Is there any way, short of sending many samples off to be examined, and then evaluating the results, to eliminate one or more of these units? Is there one that stands out for versatility or ease of use? How big a task is sample prep for these units? Additionally, have we missed a system to consider?
Please note that we have never looked at any of these components at higher than optical magnification before, so I can't tell you what features I need to see, because I have no idea what, if anything, that there is to see, or even whether or not the nanoscale detail makes a difference in the performance of our products. We will not be able to dedicate a person to the operation and maintenance of the system, so anything that takes much more than an hour or so of maintenance a week is going to be a big problem. I also need to point out that the closer the price gets to $100,000, the more competitive it is among all the instrumentation options we're considering, and the more likely it is to get approved.
Robert Zonis Product Development Chemist, LMTC Sanford L.P. - A Newell Rubbermaid Company 3 Sharpie Way Shelbyville, TN 37160 robert.zonis-at-sanford.com www.papermate.com
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==============================Original Headers============================== 10, 34 -- From Robert.Zonis-at-sanford.com Wed Jun 6 10:48:53 2007 10, 34 -- Received: from mail42.messagelabs.com (mail42.messagelabs.com [216.82.244.163]) 10, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l56FmpNJ030079 10, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 10:48:52 -0500 10, 34 -- X-VirusChecked: Checked 10, 34 -- X-Env-Sender: Robert.Zonis-at-sanford.com 10, 34 -- X-Msg-Ref: server-23.tower-42.messagelabs.com!1181144927!38267531!1 10, 34 -- X-StarScan-Version: 5.5.12.11; banners=sanford.com,-,- 10, 34 -- X-Originating-IP: [12.2.115.11] 10, 34 -- Received: (qmail 21873 invoked from network); 6 Jun 2007 15:48:47 -0000 10, 34 -- Received: from gnat.newellco.com (HELO nafepncsvpout2.nr.ad.newellco.com) (12.2.115.11) 10, 34 -- by server-23.tower-42.messagelabs.com with SMTP; 6 Jun 2007 15:48:47 -0000 10, 34 -- Received: from nafepncsefe02.nr.ad.newellco.com (unverified) by nafepncsvpout2.nr.ad.newellco.com 10, 34 -- (Content Technologies SMTPRS 4.3.17) with ESMTP id {T800d0f66ca0a059a211440-at-nafepncsvpout2.nr.ad.newellco.com} for {Microscopy-at-microscopy.com} ; 10, 34 -- Wed, 6 Jun 2007 10:48:47 -0500 10, 34 -- Received: from nashbsasebe01.nr.ad.newellco.com ([10.217.223.198]) by nafepncsefe02.nr.ad.newellco.com with Microsoft SMTPSVC(6.0.3790.1830); 10, 34 -- Wed, 6 Jun 2007 10:48:46 -0500 10, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 34 -- Content-class: urn:content-classes:message 10, 34 -- MIME-Version: 1.0 10, 34 -- Content-Type: text/plain; 10, 34 -- charset="iso-8859-1" 10, 34 -- Subject: New microscope purchase advice 10, 34 -- Date: Wed, 6 Jun 2007 10:48:45 -0500 10, 34 -- Message-ID: {8809C39726DC5F43824A3D3BE5D0286A0CAF0171-at-nashbsasebe01.nr.ad.newellco.com} 10, 34 -- X-MS-Has-Attach: 10, 34 -- X-MS-TNEF-Correlator: 10, 34 -- Thread-Topic: New microscope purchase advice 10, 34 -- Thread-Index: AceoUip05xeNL5+4QlCCM1LmkcEF1A== 10, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-sanford.com} 10, 34 -- To: {Microscopy-at-microscopy.com} 10, 34 -- X-OriginalArrivalTime: 06 Jun 2007 15:48:46.0866 (UTC) FILETIME=[2B2F6720:01C7A852] 10, 34 -- Content-Transfer-Encoding: 8bit 10, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l56FmpNJ030079 ==============================End of - Headers==============================
I would encourage you to investigate NT-MDT's Tomo, a unique combination of AFM and ultramicrotome, fitted with Media Cybernetics 3D constructor. It will provide the 3D information you need for accurate particle sizing/shape characterization as well as distribution calculations. Depending on how they are mounted, you can also use the AFM to characterize the surfaces you described, without having to coat or pump down a vacuum. Their new dealer in the US is Abeam, in Castro Valley, CA.
Also, depending on the size of the surface features, a good reflected light optical microscope, preferably fitted DIC or Hoffman Modulation Contrast, and Polarizers, would go a long way toward routine evaluations. MME provides specialized courses in both these areas. If you are interested, please contact me off-line.
CAVEAT: MME no longer has any financial relationship with NT-MDT but does make its living through customized, on-site courses.
Hope this was helpful, Barbara Foster, President
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
MME is now scheduling customized, on-site courses through September. Call us today for details.
P. S. Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 10:57 AM 6/6/2007, Robert.Zonis-at-sanford.com wrote:
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==============================Original Headers============================== 16, 17 -- From bfoster-at-mme1.com Wed Jun 6 12:23:44 2007 16, 17 -- Received: from mail.plhosting.com (qmail1e.plhosting.com [65.39.254.7]) 16, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56HNhHM016224 16, 17 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 12:23:44 -0500 16, 17 -- Message-Id: {200706061723.l56HNhHM016224-at-ns.microscopy.com} 16, 17 -- Received: (qmail 5612 invoked by uid 0); 6 Jun 2007 17:23:43 -0000 16, 17 -- Received: from unknown (HELO barbsd505.mme1.com) (bfoster-at-mme1.com-at-65.17.57.99) 16, 17 -- by qmail1e.plhosting.com with ESMTPA; 6 Jun 2007 17:23:42 -0000 16, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 16, 17 -- Date: Wed, 06 Jun 2007 12:23:06 -0500 16, 17 -- To: Robert.Zonis-at-sanford.com, microscopy-at-microscopy.com 16, 17 -- From: Barbara Foster {bfoster-at-mme1.com} 16, 17 -- Subject: Re: [Microscopy] New microscope purchase advice 16, 17 -- In-Reply-To: {200706061551.l56Fpi9p001428-at-ns.microscopy.com} 16, 17 -- References: {200706061551.l56Fpi9p001428-at-ns.microscopy.com} 16, 17 -- Mime-Version: 1.0 16, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Every once in a while we get a request to image particles which have been ingested by organisms, but this latest one seems especially tricky.
We are being asked to image carbon nanomaterials, such as nanowires, and how they distribute themselves in the tissues and organs of living organisms. My first search of the literature (ongoing, by the way) indicates that I'm not the only one having trouble with this. Aside from the obvious problems of differentiating 3-D structures in nanometers-thick sections, there is the problem of seeing low-contrast carbon in carbon-rich tissues.
My first thoughts were that adding electron density to the carbon nanothingys would make them easier to see, both in thin sections and in fractured specimens for SEM. At least this could help localize them, even if seeing their true shapes remains a problem. How to do this is another thing. Probably the particles would have to be augmented with metals before being released into the organisms' environment, and that would be a task for the makers and users of the nanoparticles. And would that affect their final distribution in the creatures? From our end, I'm trying to think of how to adapt immunolabeling techniques to this.
Has anybody run across this problem and have thoughts they'd be willing to share? The PI on this one has high hopes that we can put the nano-rabbit of this tiny, little hat.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 8, 23 -- From TindallR-at-missouri.edu Wed Jun 6 15:14:44 2007 8, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56KEhpv004593 8, 23 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 15:14:44 -0500 8, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 23 -- Wed, 6 Jun 2007 15:14:43 -0500 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="us-ascii" 8, 23 -- Subject: Carbon nanos in tissue: SEM/TEM 8, 23 -- Date: Wed, 6 Jun 2007 15:14:43 -0500 8, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BAC6-at-UM-XMAIL08.um.umsystem.edu} 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- Thread-Topic: Carbon nanos in tissue: SEM/TEM 8, 23 -- Thread-Index: Aceod1H9AEeN4khFSeSkBwoNR5LQVQ== 8, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 8, 23 -- To: {microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 06 Jun 2007 20:14:43.0146 (UTC) FILETIME=[51DE82A0:01C7A877] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l56KEhpv004593 ==============================End of - Headers==============================
As mentioned earlier, I have a movie made by Dr.deWith of the Dutch Polymer Institute (TU/E) showing the distribution of carbon nanotubes in epoxy. Because the AFM uses local differences in elasticity, there is none of the CNT in C-based tissue problem described below. Again, although I am no longer involved in this project, I am happy to forward a copy of the film to anyone who is interested. (It will come via www.YouSendIt.com, because of the large file size).
Dr. Anton Efimov of NT-MDT is the developer of this technique and is usually willing to run samples, schedule permitting. See his email in the CC: above.
NTA is no longer NT-MDT's agent here in the US. If you are interested in following this further, I recommend that you contact Dr. Yuri Bobrov, who is now with ABeam (Dealer principle, Dr. Sergey Babin - see email above).
As always, if there are further questions, please don't hesitate to call/email.
(Note: MME is no longer involved with this vendor).
Hope this was helpful, Barbara Foster, President
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
MME is now scheduling customized, on-site courses through September. Call us today for details.
P. S. Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 03:27 PM 6/6/2007, TindallR-at-missouri.edu wrote:
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==============================Original Headers============================== 20, 17 -- From bfoster-at-mme1.com Wed Jun 6 15:34:13 2007 20, 17 -- Received: from mail.plhosting.com (qmail1e.plhosting.com [65.39.254.7]) 20, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56KYDJZ016549 20, 17 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 15:34:13 -0500 20, 17 -- Message-Id: {200706062034.l56KYDJZ016549-at-ns.microscopy.com} 20, 17 -- Received: (qmail 15717 invoked by uid 0); 6 Jun 2007 20:34:12 -0000 20, 17 -- Received: from unknown (HELO barbsd505.mme1.com) (bfoster-at-mme1.com-at-65.17.57.99) 20, 17 -- by qmail1e.plhosting.com with ESMTPA; 6 Jun 2007 20:34:12 -0000 20, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 20, 17 -- Date: Wed, 06 Jun 2007 15:33:27 -0500 20, 17 -- To: TindallR-at-missouri.edu, microscopy-at-microscopy.com 20, 17 -- From: Barbara Foster {bfoster-at-mme1.com} 20, 17 -- Subject: Re: [Microscopy] Carbon nanos in tissue: SEM/TEM 20, 17 -- In-Reply-To: {200706062016.l56KGvAo008316-at-ns.microscopy.com} 20, 17 -- References: {200706062016.l56KGvAo008316-at-ns.microscopy.com} 20, 17 -- Mime-Version: 1.0 20, 17 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
I'm VERY interested. We need a database of dedicated people who are doing high school SEM, so that experience, problems, curricula, etc. can be shared. Will either of you be at M&M in Ft. Lauderdale? Please come to the MICRO booth so that we can try to organize something. Or send me an Email. If anyone else who is doing HS SEM, including those associated with the FEI & RJ Lee school SEM programs, is reading this, we need to talk to you too!
What is my motive, other than trying to get you together? Project MICRO is MSA's outreach program for middle schools (see URL below). I'd like to convince all of you to encourage introductory LM in your "feeder" middle schools; SEM is an abrupt way to begin.
Caroline
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==============================Original Headers============================== 4, 19 -- From schooley-at-mcn.org Wed Jun 6 16:16:27 2007 4, 19 -- Received: from dns3.mcn.org (dns3.mcn.org [216.150.240.32]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56LGQ0V028666 4, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 6 Jun 2007 16:16:27 -0500 4, 19 -- Received: from [66.52.170.6] (helo=[10.0.1.2]) 4, 19 -- by dns3.mcn.org with esmtpa (Exim 4.60) 4, 19 -- (envelope-from {schooley-at-mcn.org} ) 4, 19 -- id JJ8GF6-000LT6-59; Wed, 06 Jun 2007 14:16:22 -0700 4, 19 -- Mime-Version: 1.0 4, 19 -- Message-Id: {a06200701c28ccf4911d0-at-[10.0.1.2]} 4, 19 -- In-Reply-To: {200706061316.l56DGlWV025688-at-ns.microscopy.com} 4, 19 -- References: {200706061316.l56DGlWV025688-at-ns.microscopy.com} 4, 19 -- Date: Wed, 6 Jun 2007 14:18:01 -0700 4, 19 -- To: dljones-at-bestweb.net, donovan.leonard-at-gmail.com 4, 19 -- From: Caroline Schooley {schooley-at-mcn.org} 4, 19 -- Subject: [Microscopy] Re: Table Top SEM & High School Nano Course 4, 19 -- Cc: Microscopy-at-MSA.Microscopy.Com, john_mackenzie-at-ncsu.edu, 4, 19 -- tpepper-at-iastate.edu, murphyjudy-at-comcast.net 4, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Has the M&M 2007 daily schedule of papers and symposia been published yet? I didn't find it on the meeting web site?
thanks, Henk
Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 6, 23 -- From colijn.1-at-osu.edu Wed Jun 6 16:18:26 2007 6, 23 -- Received: from ER6S1.ENG.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56LIPD0032266 6, 23 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 16:18:26 -0500 6, 23 -- Received: from CONVERSION-DAEMON.er6s1.eng.ohio-state.edu by 6, 23 -- er6s1.eng.ohio-state.edu (PMDF V6.2-1x11 #31056) 6, 23 -- id {01MHH5RIFKUOAA9XBW-at-er6s1.eng.ohio-state.edu} for 6, 23 -- microscopy-at-microscopy.com; Wed, 06 Jun 2007 17:18:24 -0400 (EDT) 6, 23 -- Received: from HOC1.ecr6.ohio-state.edu 6, 23 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.eng.ohio-state.edu 6, 23 -- (PMDF V6.2-1x11 #31056) 6, 23 -- with ESMTPA id {01MHH5RI2SAUA9PSMS-at-er6s1.eng.ohio-state.edu} for 6, 23 -- microscopy-at-microscopy.com; Wed, 06 Jun 2007 17:18:24 -0400 (EDT) 6, 23 -- Date: Wed, 06 Jun 2007 17:19:11 -0400 6, 23 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 6, 23 -- Subject: M&M 2007 detailed program 6, 23 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 6, 23 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 6, 23 -- Message-id: {7.0.1.0.2.20070606171402.036b0d80-at-osu.edu} 6, 23 -- MIME-version: 1.0 6, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 6, 23 -- Content-type: text/plain; charset=us-ascii; format=flowed 6, 23 -- X-Env-From: auth/colijn.1-at-osu.edu ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jenniferwal-at-cs.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, June 6, 2007 at 13:16:12 ---------------------------------------------------------------------------
Email: jenniferwal-at-cs.com Name: Jennifer Wallace
Organization: Academic Coaching
Education: 9-12th Grade High School
Location: Evanston, IL USA
Question: I am working with a junior in high school who is very interested in this field. We are trying to find an internship or volunteer experience for her and need help! She is in the most rigorous science program at her high school and is an excellent photographer, which is why she is interested in this field in college and beyond.
I know that vendors/vendor employees are active and involved in the MSA and contribute greatly to the dialogs here. However, I personally feel that this message reply is a bit over the top in commercializing the List. Every question to the List can't be answered "you should try AFM" and go on to plug the company and it's products. The response seems only slightly related to the essence of the original question. How is AFM going to visualize carbon nanotubes inside a cell that is inside a tissue? This was a repeat of the response to recent technical questions on vacuum metal shadowing technical problems. It is fine to bring attention to alternate methods, but primarily we should be good listeners and try to help people with the problem they have asked about. Metal shadowing is a proven technique - with limitations, same as ALL techniques - that people use routinely; one can scan relatively large areas at high resolution for the best area. It is a method suitable to the purpose. The DNA is already bulked up artificially by the preparation method and the metal shadowing adds a known factor to that size. But {visualizing} the DNA/plasmid is often the key, not the exact molecular size. One vendor sent a link to an image of a 1um square scan of DNA; the image was quite blurry, 2 nm/pixel resolution. I'm sure this is a "display" result - the best they have. Finding a plasmid on a sheet of carbon/mica for AFM scanning can't be as routine as the "traditional" method of viewing the shadowed material in a TEM. It is a bit of comparing apples and oranges.
So AFM is not the one answer to all questions, and please go light on the commercial plugs. It seems that vendors were more respectful of this "line" in the past.
Thanks for allowing my rant,
Dale Callaham
bfoster-at-mme1.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Randy, } } This is another one of those "TOMO" applications. } } As mentioned earlier, I have a movie made by Dr.deWith of the Dutch Polymer Institute (TU/E) showing the distribution of carbon nanotubes in epoxy. Because the AFM uses local differences in elasticity, there is none of the CNT in C-based tissue problem described below. Again, although I am no longer involved in this project, I am happy to forward a copy of the film to anyone who is interested. (It will come via www.YouSendIt.com, because of the large file size). } } Dr. Anton Efimov of NT-MDT is the developer of this technique and is usually willing to run samples, schedule permitting. See his email in the CC: above. } } NTA is no longer NT-MDT's agent here in the US. If you are interested in following this further, I recommend that you contact Dr. Yuri Bobrov, who is now with ABeam (Dealer principle, Dr. Sergey Babin - see email above). } } As always, if there are further questions, please don't hesitate to call/email. } } (Note: MME is no longer involved with this vendor). } } Hope this was helpful, } Barbara Foster, President } } Microscopy/Microscopy Education } 313 S Jupiter Rd, Suite 100 } Allen, TX 75002 } P: 972-954-8011 } W: www.MicroscopyEducation.com } } } MME is now scheduling customized, on-site courses through September. Call us today for details. } } P. S. } Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011. } } } } } At 03:27 PM 6/6/2007, TindallR-at-missouri.edu wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear Listers, } } } } Every once in a while we get a request to image particles which have } } been ingested by organisms, but this latest one seems especially tricky. } } } } We are being asked to image carbon nanomaterials, such as nanowires, and } } how they distribute themselves in the tissues and organs of living } } organisms. My first search of the literature (ongoing, by the way) } } indicates that I'm not the only one having trouble with this. Aside } } from the obvious problems of differentiating 3-D structures in } } nanometers-thick sections, there is the problem of seeing low-contrast } } carbon in carbon-rich tissues. } } } } My first thoughts were that adding electron density to the carbon } } nanothingys would make them easier to see, both in thin sections and in } } fractured specimens for SEM. At least this could help localize them, } } even if seeing their true shapes remains a problem. How to do this is } } another thing. Probably the particles would have to be augmented with } } metals before being released into the organisms' environment, and that } } would be a task for the makers and users of the nanoparticles. And } } would that affect their final distribution in the creatures? From our } } end, I'm trying to think of how to adapt immunolabeling techniques to } } this. } } } } Has anybody run across this problem and have thoughts they'd be willing } } to share? The PI on this one has high hopes that we can put the } } nano-rabbit of this tiny, little hat. } } } } Cheers, } } Randy } } } } Randy Tindall } } Senior EM Specialist } } Electron Microscopy Core Facility---We Do Small Well! } } W125 Veterinary Medicine } } University of Missouri } } Columbia, MO 65211 } } Tel: (573) 882-8304 } } Fax: (573) 884-2227 } } Email: tindallr-at-missouri.edu } } Web: http://www.emc.missouri.edu } } On-line calendar: } } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } } Week&NavType=Both&Type=TimePlan } } } } } } ==============================Original Headers============================== } } 8, 23 -- From TindallR-at-missouri.edu Wed Jun 6 15:14:44 2007 } } 8, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) } } 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56KEhpv004593 } } 8, 23 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 15:14:44 -0500 } } 8, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } } 8, 23 -- Wed, 6 Jun 2007 15:14:43 -0500 } } 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } } 8, 23 -- Content-class: urn:content-classes:message } } 8, 23 -- MIME-Version: 1.0 } } 8, 23 -- Content-Type: text/plain; } } 8, 23 -- charset="us-ascii" } } 8, 23 -- Subject: Carbon nanos in tissue: SEM/TEM } } 8, 23 -- Date: Wed, 6 Jun 2007 15:14:43 -0500 } } 8, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BAC6-at-UM-XMAIL08.um.umsystem.edu} } } 8, 23 -- X-MS-Has-Attach: } } 8, 23 -- X-MS-TNEF-Correlator: } } 8, 23 -- Thread-Topic: Carbon nanos in tissue: SEM/TEM } } 8, 23 -- Thread-Index: Aceod1H9AEeN4khFSeSkBwoNR5LQVQ== } } 8, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } } 8, 23 -- To: {microscopy-at-microscopy.com} } } 8, 23 -- X-OriginalArrivalTime: 06 Jun 2007 20:14:43.0146 (UTC) FILETIME=[51DE82A0:01C7A877] } } 8, 23 -- Content-Transfer-Encoding: 8bit } } 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l56KEhpv004593 } } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 20, 17 -- From bfoster-at-mme1.com Wed Jun 6 15:34:13 2007 } 20, 17 -- Received: from mail.plhosting.com (qmail1e.plhosting.com [65.39.254.7]) } 20, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56KYDJZ016549 } 20, 17 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 15:34:13 -0500 } 20, 17 -- Message-Id: {200706062034.l56KYDJZ016549-at-ns.microscopy.com} } 20, 17 -- Received: (qmail 15717 invoked by uid 0); 6 Jun 2007 20:34:12 -0000 } 20, 17 -- Received: from unknown (HELO barbsd505.mme1.com) (bfoster-at-mme1.com-at-65.17.57.99) } 20, 17 -- by qmail1e.plhosting.com with ESMTPA; 6 Jun 2007 20:34:12 -0000 } 20, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 20, 17 -- Date: Wed, 06 Jun 2007 15:33:27 -0500 } 20, 17 -- To: TindallR-at-missouri.edu, microscopy-at-microscopy.com } 20, 17 -- From: Barbara Foster {bfoster-at-mme1.com} } 20, 17 -- Subject: Re: [Microscopy] Carbon nanos in tissue: SEM/TEM } 20, 17 -- In-Reply-To: {200706062016.l56KGvAo008316-at-ns.microscopy.com} } 20, 17 -- References: {200706062016.l56KGvAo008316-at-ns.microscopy.com} } 20, 17 -- Mime-Version: 1.0 } 20, 17 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 21 -- From dac-at-research.umass.edu Thu Jun 7 10:40:53 2007 8, 21 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57FerNb024904 8, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 10:40:53 -0500 8, 21 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 8, 21 -- (authenticated bits=0) 8, 21 -- by race4.oit.umass.edu (8.13.7/8.13.7) with ESMTP id l57FeqlA029015 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 11:40:52 -0400 8, 21 -- Message-ID: {4668359D.3010902-at-research.umass.edu} 8, 21 -- Date: Thu, 07 Jun 2007 11:43:09 -0500 8, 21 -- From: Dale Callaham {dac-at-research.umass.edu} 8, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.4) Gecko/20070509 SeaMonkey/1.1.2 8, 21 -- MIME-Version: 1.0 8, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 8, 21 -- Subject: Re: [Microscopy] Re: Carbon nanos in tissue: SEM/TEM 8, 21 -- References: {200706062039.l56Kd7jT026138-at-ns.microscopy.com} 8, 21 -- In-Reply-To: {200706062039.l56Kd7jT026138-at-ns.microscopy.com} 8, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 21 -- Content-Transfer-Encoding: 7bit 8, 21 -- X-Whitelist: TRUE ==============================End of - Headers==============================
MSA 2007 is just a short 8 weeks away in beautiful Ft. Lauderdale, Florida !!!!
The reduced rate registration deadline is July 6, so please register early.
This is the second announcement for students who will attend the Microscopy and Microanalysis Meeting in Ft. Lauderdale, Fl, August 5-9, 2007 and would like to apply for a Student Bursary to help defray meeting costs. The complete description of the Bursary is listed below, including the registration process.
In addition, technicians, post-docs, faculty and presenters may also apply for a bursary in exchange for working to support the meeting.
The contact persons this year who will coordinate the student volunteers are;
Bill Monroe monroe-at-emcenter.msstate.edu Amanda Lawrence alawrence-at-emcenter.msstate.edu Mike Miller millem1-at-auburn.edu
STUDENT BURSARIES
The Microscopy Society of America values student members and recognizes that they are the future of both the society and the field of microscopy in general. MSA is therefore pleased to offer student bur-saries of $200 to registered students. The most important purpose of these bursaries is to encourage students to attend the annual MSA/MAS Microscopy and Microanalysis meeting, where these young sci-entists can meet and interact with the established microscopy community as well as assisting with the meeting.
Each bursary recipient will be expected to work for a minimum of 20 hours during the meeting and/or at the pre-meeting weekend events. A student may work up to an additional 20 hours, for a total of 40 hours. These extra hours will add to the bursary total at a rate of $10 per hour. The maximum bursary will therefore be $400. The duties will involve, but are not necessarily limited to, providing support in symposia (helping with audio-visual needs, maintaining an attendance count, and helping speakers set up for their presentation), staffing the MSA Megabooth, and monitoring use of the Internet Cafe. Applicants for the bursaries must be members of MSA or MAS, and enrolled as students at a recognized educational institution. All MSA or MAS student members are eligible for bursaries, including those who are recipients of MSA Presidential Scholars Awards and MAS Distinguished Scholar Awards. Eligible students may apply for bursaries when registering for the conference on the conference website, or at on-site registration. Bursaries are limited and early application is encouraged. How it works:
The registration form for the meeting will have a check box indicating that the applicant is a registered student and is requesting a bursary. The check box will have a note beside it reminding the applicant that the bursary requires them to work at the meeting. Students who have applied for bursaries will receive letters from MSA explaining the conditions that they need to satisfy in order to receive the bursaries. The tasks at the meeting will be allocated by the Student Worker Organizers Sub-Committee of the Education Committee. When students pick up their registration materials at the meeting, they will receive assignment forms indicating the specific tasks they are to perform, and the person(s) they need to contact in order to carry out those tasks. Each stu-dent's assignment forms must be signed by all of the contact persons listed, indicating that all assigned tasks have been performed. Upon completion of assignments and submission of the signed forms, the bursary check will be issued by the appropriate representative at the registration desk.
Should you have questions concerning the process, please contact:
Bill Monroe monroe-at-emcenter.msstate.edu
-- Bill Monroe Electron Microscope Center 103 Clay Lyle Entomology Building Mississippi State University Mississippi State, MS 39762 (662)-325-3019 Work (662)-323-5246 Home (662)-325-0246 Fax
==============================Original Headers============================== 17, 15 -- From monroe-at-emcenter.msstate.edu Thu Jun 7 10:52:13 2007 17, 15 -- Received: from Ra.MsState.Edu (Ra.msstate.edu [130.18.80.10]) 17, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57FqC7o004127 17, 15 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 10:52:12 -0500 17, 15 -- Received: from [130.18.130.108] (ws108-130.clay-lyle.dynamic.msstate.edu [130.18.130.108]); 17, 15 -- by Ra.MsState.Edu (8.13.8/8.12.8/ra_1.2) with SMTP; 17, 15 -- id l57FqBOG005896 for {"Microscopy-at-microscopy.com"} ; Thu, 7 Jun 2007 10:52:12 -0500 (CDT) 17, 15 -- Mime-Version: 1.0 17, 15 -- Message-Id: {a06230934c28dd7b91c59-at-[130.18.130.108]} 17, 15 -- Date: Thu, 7 Jun 2007 10:52:11 -0500 17, 15 -- To: "Microscopy-at-microscopy.com"-at-Ra.MsState.Edu 17, 15 -- From: "William A. Monroe" {monroe-at-emcenter.msstate.edu} 17, 15 -- Subject: MSA 2007 Meeting: Request for Student and Other Volunteers 17, 15 -- (Bursaries Available) 17, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
To clarify: I am a consultant who has exposure to new technology and, if you review my messages over the years, you will find a large number of different technologies and vendors mentioned.
I did have a chance to work with NT-MDT (no longer)... and found that AFM, like other technologies (ex: interferometry) was under-utilized.
Regarding giving a plug to one vendor: TOMO is unique. There is no one else has integrated an AFM with an ultramicrotome for serial sections... so there IS no one else to mention.
Hope this is helpful.
Barbara
At 10:44 AM 6/7/2007, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 14, 17 -- From bfoster-at-mme1.com Thu Jun 7 11:10:59 2007 14, 17 -- Received: from mail.plhosting.com (qmail1h.plhosting.com [65.39.254.134]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57GAwf8015890 14, 17 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 11:10:59 -0500 14, 17 -- Message-Id: {200706071610.l57GAwf8015890-at-ns.microscopy.com} 14, 17 -- Received: (qmail 1085 invoked by uid 0); 7 Jun 2007 16:10:57 -0000 14, 17 -- Received: from unknown (HELO barbsd505.mme1.com) (bfoster-at-mme1.com-at-65.17.57.99) 14, 17 -- by qmail1h.plhosting.com with ESMTPA; 7 Jun 2007 16:10:56 -0000 14, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 14, 17 -- Date: Thu, 07 Jun 2007 11:10:22 -0500 14, 17 -- To: dac-at-research.umass.edu, microscopy-at-microscopy.com 14, 17 -- From: Barbara Foster {bfoster-at-mme1.com} 14, 17 -- Subject: Re: [Microscopy] Carbon nanos in tissue: SEM/TEM 14, 17 -- In-Reply-To: {200706071544.l57Fi3p9028702-at-ns.microscopy.com} 14, 17 -- References: {200706071544.l57Fi3p9028702-at-ns.microscopy.com} 14, 17 -- Mime-Version: 1.0 14, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both oddioeng-at-aol.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: oddioeng-at-aol.com Name: J. Allen Williams, Jr.
Organization: ORDELA, Inc.
Title-Subject: [Filtered] UPDATE of UC-4 Dewar Re-evacuation port
Question: Hello, I have discovered the answer to my question from yesterday, the ball in the evacuation port is the vacuum valve for the Dewar. If the Dewar is near atmosphere and if one needs to re-evacuate, you may use either a specified valve or what I did - (which is somewhat unorthodox) - connect your evaluation pump to the port and the ball 'magically levitates' from the o-ring seal. (This is also true of smaller LINDE Dewars). Therefore, the main seal of this type Dewar re-evacuation port seals the vacuum inside of the vacuum insulator by the atmospheric pressure on the ball and the o-ring seat.
I found this out by connecting my old Welch 1402 vacuum pump to the Dewar port and heard a click, and the click was a sound of the ball being sucked towards the vacuum pump. Mystery solvÈd. Thought I would let everyone know. It is amazing that atmospheric pressure can seal the vacuum in a liquid nitrogen/argon Dewar. Thanks for all the suggestions and hope this may help for reference in the future.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both K.venner-at-ion.ucl.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ecd10-at-psu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ecd10-at-psu.edu Name: Elizabeth Dickey
Organization: Pennsylvania State University
Title-Subject: [Filtered] Postdoctoral Position Open
Question: POST-DOCTORAL POSITION in Transmission Electron Microscopy at The Pennsylvania State University
A postdoctoral position is available in the area of transmission electron microscopy of amorphous dielectrics beginning July 1, 2007. The research project focuses on understanding structure and chemistry of amorphous metal oxides for capacitor applications. Through a variety of electron imaging, spectroscopy and diffraction techniques, including fluctuation electron microscopy, we aim to quantify structure and chemistry of amorphous metal-oxide layers to help establish processing/structure/property relationships for this class of materials. Most of the research will be conducted on a JEOL 2010F Field Emission TEM/STEM outfitted with an EDAX energy dispersive x-ray spectrometer (EDS), Gatan Enfina electron energy loss spectrometer (EELS), high-angle annular dark field STEM detector, and acquisition hardware and software for spectrum imaging. The ideal candidate for this position will have experience in HREM, STEM, EDS and EELS. The salary will be commensurate with qualifications and experience.
Please forward questions or send applications to:
Professor Elizabeth Dickey Department of Materials Science and Engineering The Pennsylvania State University 223 Materials Research Building University Park, PA 16802 USA
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both skooi-at-mit.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: skooi-at-mit.edu Name: Steven Kooi
Organization: ISN -at- MIT
Title-Subject: [Filtered] Research Specialist Position
Question: The Institute for Soldier Nanotechnologies (ISN) at MIT is looking for a research specialist to handle multiple responsibilities including transmission electron microscopy (TEM), scanning electron microscopy (SEM), focused ion beam (FIB), and atomic force microscopy (AFM). Primary responsibilities include providing introductory and more advanced training of users on electron microscopes, and assisting ISN researchers with sample preparation and characterization using electron and surface microscopy techniques. Other responsibilities may include daily operation, maintenance, and upkeep of several pieces of advanced nano-characterization instrumentation and related sample preparation tools; and serving as the primary contact with the equipment manufacturer's service personnel to quickly resolve any serious instrumentation issues.
The position announcement can be found at http://sh.webhire.com/servlet/av/jd?ai=631&ji=2025576&sn=I
Additional information on ISN is available at http://web.mit.edu/isn.
I agree that keeping the list as "commercial free" as possible is a good thing. However, I didn't really interpret Barbara's response to my question as a commercial plug as much as pointing out a potentially useful technology. In fact, I believe she pointed out that MME is no longer affiliated with that vendor. Personally, I'll take any advice that helps our lab help our clients with their research, whether from consultants, vendors, or fellow techs.
I have had many vendors contact me off-list with suggestions involving their products, when they thought their responses might be seen as too commercial. To me that's a very courteous and professional response. That said, I hope that those who are not formally affiliated with a vendor won't hesitate to point out useful techniques or gizmos to the list at large.
My personal opinion is that this particular response was not out of line.
It's good to bring these issues up occasionally, so this is not a dig at Dale, by any means.
Cheers, Randy
-----Original Message----- X-from: dac-at-research.umass.edu [mailto:dac-at-research.umass.edu] Sent: Thursday, June 07, 2007 10:42 AM To: Tindall, Randy D.
Dear Microscopists,
I know that vendors/vendor employees are active and involved in the MSA and contribute greatly to the dialogs here. However, I personally feel that this message reply is a bit over the top in commercializing the List. Every question to the List can't be answered "you should try AFM" and go on to plug the company and it's products. The response seems only slightly related to the essence of the original question. How is AFM going to visualize carbon nanotubes inside a cell that is inside a tissue? This was a repeat of the response to recent technical questions on vacuum metal shadowing technical problems. It is fine to bring attention to alternate methods, but primarily we should be good listeners and try to help people with the problem they have asked about.
Metal shadowing is a proven technique - with limitations, same as ALL techniques - that people use routinely; one can scan relatively large areas at high resolution for the best area. It is a method suitable to the purpose. The DNA is already bulked up artificially by the preparation method and the metal shadowing adds a known factor to that size. But {visualizing} the DNA/plasmid is often the key, not the exact molecular size. One vendor sent a link to an image of a 1um square scan of DNA; the image was quite blurry, 2 nm/pixel resolution. I'm sure this is a "display" result - the best they have. Finding a plasmid on a sheet of carbon/mica for AFM scanning can't be as routine as the "traditional"
method of viewing the shadowed material in a TEM. It is a bit of comparing apples and oranges.
So AFM is not the one answer to all questions, and please go light on the commercial plugs. It seems that vendors were more respectful of this "line" in the past.
Thanks for allowing my rant,
Dale Callaham
bfoster-at-mme1.com wrote: } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Randy, } } This is another one of those "TOMO" applications. } } As mentioned earlier, I have a movie made by Dr.deWith of the Dutch Polymer Institute (TU/E) showing the distribution of carbon nanotubes in epoxy. Because the AFM uses local differences in elasticity, there is none of the CNT in C-based tissue problem described below. Again, although I am no longer involved in this project, I am happy to forward a copy of the film to anyone who is interested. (It will come via www.YouSendIt.com, because of the large file size). } } Dr. Anton Efimov of NT-MDT is the developer of this technique and is usually willing to run samples, schedule permitting. See his email in the CC: above. } } NTA is no longer NT-MDT's agent here in the US. If you are interested in following this further, I recommend that you contact Dr. Yuri Bobrov, who is now with ABeam (Dealer principle, Dr. Sergey Babin - see email above). } } As always, if there are further questions, please don't hesitate to call/email. } } (Note: MME is no longer involved with this vendor). } } Hope this was helpful, } Barbara Foster, President } } Microscopy/Microscopy Education } 313 S Jupiter Rd, Suite 100 } Allen, TX 75002 } P: 972-954-8011 } W: www.MicroscopyEducation.com } } } MME is now scheduling customized, on-site courses through September. Call us today for details. } } P. S. } Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011. } } } } } At 03:27 PM 6/6/2007, TindallR-at-missouri.edu wrote: } } } } } --------------------------------------------------------------------- } } ------- The Microscopy ListServer -- CoSponsor: The Microscopy } } Society of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------- } } ------- } } } } Dear Listers, } } } } Every once in a while we get a request to image particles which have } } been ingested by organisms, but this latest one seems especially tricky. } } } } We are being asked to image carbon nanomaterials, such as nanowires, } } and how they distribute themselves in the tissues and organs of } } living organisms. My first search of the literature (ongoing, by the
} } way) indicates that I'm not the only one having trouble with this. } } Aside from the obvious problems of differentiating 3-D structures in } } nanometers-thick sections, there is the problem of seeing low-contrast
} } carbon in carbon-rich tissues. } } } } My first thoughts were that adding electron density to the carbon } } nanothingys would make them easier to see, both in thin sections and } } in fractured specimens for SEM. At least this could help localize } } them, even if seeing their true shapes remains a problem. How to do } } this is another thing. Probably the particles would have to be } } augmented with metals before being released into the organisms' } } environment, and that would be a task for the makers and users of the
} } nanoparticles. And would that affect their final distribution in the
} } creatures? From our end, I'm trying to think of how to adapt } } immunolabeling techniques to this. } } } } Has anybody run across this problem and have thoughts they'd be } } willing to share? The PI on this one has high hopes that we can put } } the nano-rabbit of this tiny, little hat. } } } } Cheers, } } Randy } } } } Randy Tindall } } Senior EM Specialist } } Electron Microscopy Core Facility---We Do Small Well! } } W125 Veterinary Medicine } } University of Missouri } } Columbia, MO 65211 } } Tel: (573) 882-8304 } } Fax: (573) 884-2227 } } Email: tindallr-at-missouri.edu } } Web: http://www.emc.missouri.edu } } On-line calendar: } } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amou } } nt= } } Week&NavType=Both&Type=TimePlan } } } } } } ==============================Original } } Headers============================== } } 8, 23 -- From TindallR-at-missouri.edu Wed Jun 6 15:14:44 2007 8, 23 --
} } Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) } } 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56KEhpv004593 } } 8, 23 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 15:14:44 -0500 } } 8, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } } 8, 23 -- Wed, 6 Jun 2007 15:14:43 -0500 } } 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- } } Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0
I'd like to convince all of you to encourage inductory imaging and measurement in your "feeder" middle schools. It has been my experience that too few students understand anything about shape and size. Those who are unable to measure the diameter and length of a broomstick are going to have a difficult time on a nano-rod.
just my two cents............
JQuinn
PS...........OoO away........
} From mail-at-ns.microscopy.com Wed Jun 6 17:14:41 2007 } Date: Wed, 6 Jun 2007 16:17:10 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: schooley-at-mcn.org } Reply-to: schooley-at-mcn.org } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] Re: Table Top SEM & High School Nano Course } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I'm VERY interested. We need a database of dedicated people who are } doing high school SEM, so that experience, problems, curricula, etc. } can be shared. Will either of you be at M&M in Ft. Lauderdale? } Please come to the MICRO booth so that we can try to organize } something. Or send me an Email. If anyone else who is doing HS SEM, } including those associated with the FEI & RJ Lee school SEM programs, } is reading this, we need to talk to you too! } } What is my motive, other than trying to get you together? Project } MICRO is MSA's outreach program for middle schools (see URL below). } I'd like to convince all of you to encourage introductory LM in your } "feeder" middle schools; SEM is an abrupt way to begin. } } Caroline } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Donovan, } } } } I have helped get a SEM put into my local high school. The science teacher } } there now is able to offer 8 college credits to students that take his } } advanced program related to this instrument. Mark would be able to talk to } } you more about that aspect and how he integrates it into his curriculum. I } } have cc'd him with this email so you'll have his email address. This is } } still in the beginning stages so there is a lot yet to know and learn. But } } I think it is an absolutely fabulous opportunity for learning... } } } } In the process of getting the SEM into my local high school, I have } } learned a fair amount about SEM usage in high schools in the US and a bit } } internationally. There are pockets of high schools around the USA that } } have this kind of technology operating in high schools, but not a lot. } } Germany has some high schools using it, apparently Japan has it } } extensively used throughout their high school system. } } } } You are using Au and Ag particles. My experience indicates high school } } students seem more interested in biological samples rather than materials. } } It would be very useful if a materials curriculum could be developed that } } would interest students at this level. } } } } Finding teachers and school systems that are open to this and have the } } science background to make it work well, I think is a large hurdle. If a } } solid curriculm could be put together that would work as a framework for } } teachers to use, I think that would be very helpful. } } } } Schools are, justifiably, reluctant to use this technology as maintaining } } these machines is quite expensive and most schools run on very tight } } budgets. In the cases where I have seen SEM's working in high schools, } } there has usually been someone that is knowledgable about the instruments } } and can maintain them for the school pro-bono. These individuals have been } } key in getting SEM's into high schools. (There may be a program in western } } PA that's an exception to this, but I've not had any luck contacting } } them.) } } } } Do send me your curriculum and further information about what you are } } doing. I can give you more details of programs I've discovered if you } } wish, but I don't think those details are of general interest to the list } } (do correct me if I'm wrong). } } } } dj } } } } On Tue, 5 Jun 2007, donovan.leonard-at-gmail.com wrote: } } } } } Hello Listsers - } } } } } } Some days ago there was a question posed about the educational benefits } } } of table top SEM and nano-scale samples. In a few weeks I'll post my } } } experiences using a Hitachi TM-1000 table top SEM in a Nanotechnology } } } course for high schoolers as part of the Duke University Talent } } } Identification Program (TIP). More about the program can be found at } } } 'www.tip.duke.edu'. I've chosen the TM-1000 to introduce the students } } } to the micro and nanoscale, and we will be using the SEM, in addition to } } } AFM and HRTEM to characterize Au and Ag nanoparticles the students } } } synthesize as part of a experiential learning activity. } } } } } } In my opinion the table top SEM will allow the students hands-on } } } experience with an electron microscope. Not that it will resolve atomic } } } columns in nanoparticles, but as a way to introduce them to the scale of } } } things. The intention is to let the students choose ANY samples they } } } are interested in and let them discover the detail of features on the } } } micro and nanoscale. } } } } } } If anyone has had experience integrating these microscopies into a high } } } school course on nanotechnology I would be most interested in learning } } } more about how it was accomplished and the outcomes. I would also be } } } more than happy to share the syllabus created for the course if requested. } } } } } } Stay tuned, I'll post the student's data and feedback on the table top } } } SEM, AFM and TEM. } } } } } Donovan } -- } Caroline Schooley } Project MICRO Coordinator } Microscopy Society of America } Box 117, 45301 Caspar Point Road } Caspar, CA 95420 } Phone/FAX (707)964-9460 } Project MICRO: http://www.microscopy.org/ProjectMICRO } } ==============================Original Headers============================== } 4, 19 -- From schooley-at-mcn.org Wed Jun 6 16:16:27 2007 } 4, 19 -- Received: from dns3.mcn.org (dns3.mcn.org [216.150.240.32]) } 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56LGQ0V028666 } 4, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 6 Jun 2007 16:16:27 -0500 } 4, 19 -- Received: from [66.52.170.6] (helo=[10.0.1.2]) } 4, 19 -- by dns3.mcn.org with esmtpa (Exim 4.60) } 4, 19 -- (envelope-from {schooley-at-mcn.org} ) } 4, 19 -- id JJ8GF6-000LT6-59; Wed, 06 Jun 2007 14:16:22 -0700 } 4, 19 -- Mime-Version: 1.0 } 4, 19 -- Message-Id: {a06200701c28ccf4911d0-at-[10.0.1.2]} } 4, 19 -- In-Reply-To: {200706061316.l56DGlWV025688-at-ns.microscopy.com} } 4, 19 -- References: {200706061316.l56DGlWV025688-at-ns.microscopy.com} } 4, 19 -- Date: Wed, 6 Jun 2007 14:18:01 -0700 } 4, 19 -- To: dljones-at-bestweb.net, donovan.leonard-at-gmail.com } 4, 19 -- From: Caroline Schooley {schooley-at-mcn.org} } 4, 19 -- Subject: [Microscopy] Re: Table Top SEM & High School Nano Course } 4, 19 -- Cc: Microscopy-at-MSA.Microscopy.Com, john_mackenzie-at-ncsu.edu, } 4, 19 -- tpepper-at-iastate.edu, murphyjudy-at-comcast.net } 4, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
==============================Original Headers============================== 12, 12 -- From jquinn-at-www.matscieng.sunysb.edu Thu Jun 7 12:58:22 2007 12, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 12, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57HwMgC021518 12, 12 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 12:58:22 -0500 12, 12 -- Received: (from jquinn-at-localhost) 12, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id l57Hthw01791 12, 12 -- for microscopy-at-microscopy.com; Thu, 7 Jun 2007 13:55:43 -0400 12, 12 -- Date: Thu, 7 Jun 2007 13:55:43 -0400 12, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 12, 12 -- Message-Id: {200706071755.l57Hthw01791-at-www.matscieng.sunysb.edu} 12, 12 -- To: microscopy-at-microscopy.com 12, 12 -- Subject: Re: [Microscopy] Re: Table Top SEM & High School Nano Course ==============================End of - Headers==============================
Are there any really tiny cameras about, or at least, fibre-optic lenses/objectives? I ask because we have a couple of applications that could use one. We're interested in roots growing down pre-existing soil pores, and would like to watch what they're doing down there, either with a moving imaging device of some kind, or a series of portholes to watch roots growing past. Another group is screening different lines of cereals, in particular, looking at development of the floral meristem. This object of desire is enclosed in many leaves at ground level in a cereal plant, we'd like to poke a small fibre-optic in to watch its development over 3-6 days, rather than having to destroy many plants to follow the stages of development.
I've had a rummage through numerous websites, and sent off some email enquiries, but wondered if any on this list were aware of available technology to do this.
cheers, Rosemary
Dr Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 61 (0)2-6246 5475 GPO Box 1600 fax. 61 (0)2-6246 5334 Canberra, ACT 2601 Australia
==============================Original Headers============================== 5, 22 -- From Rosemary.White-at-csiro.au Thu Jun 7 18:06:13 2007 5, 22 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au [150.229.7.37]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57N6BUp006428 5, 22 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 18:06:12 -0500 5, 22 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=B/719hCOmj70x4fw8Xa9jlsNCiQ81UNL/KVQyRNlI1roNRlDs0bdsuAYvhHWzstYOtqiQHorAT8msXRgrzpp2byXutq6Cr6KK0FIId20OL2fZ/pEb92ipjpFWEHFSiHv; 5, 22 -- X-IronPort-AV: E=Sophos;i="4.16,397,1175436000"; 5, 22 -- d="scan'208";a="162041683" 5, 22 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) 5, 22 -- by act-ironport-int.csiro.au with ESMTP; 08 Jun 2007 09:06:03 +1000 5, 22 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 5, 22 -- Fri, 8 Jun 2007 09:06:10 +1000 5, 22 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 5, 22 -- Date: Fri, 08 Jun 2007 09:09:44 +1000 5, 22 -- Subject: fibre-optic probes/cameras? 5, 22 -- From: Rosemary White {Rosemary.White-at-csiro.au} 5, 22 -- To: {microscopy-at-microscopy.com} 5, 22 -- Message-ID: {C28ECD58.1DFA3%Rosemary.White-at-csiro.au} 5, 22 -- In-Reply-To: {200705311420.l4VEKqBb024233-at-ns.microscopy.com} 5, 22 -- Mime-version: 1.0 5, 22 -- Content-type: text/plain; charset="US-ASCII" 5, 22 -- Content-transfer-encoding: 7bit 5, 22 -- X-OriginalArrivalTime: 07 Jun 2007 23:06:10.0382 (UTC) FILETIME=[6FF3E6E0:01C7A958] ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both robby2-at-asu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: robby2-at-asu.edu Name: Robert W. Roberson
Organization: Arizona State University
Title-Subject: [Filtered] Position Available
Question: Research Scientist
An individual is sought, at the PhD level, who is skilled in methods of light and electron microscopy. He or she will play a key bioimaging role in a recently funded project for the development of biofuels through the manipulation of the cyanobacterium Synechocystis sp. PCC 6803. The individual will interact closely with a group of investigators within the School of Life Science and Biodesign Institute at Arizona State University. Specifically, the individual will be responsible for designing and implementing suitable protocols for microscopic analysis of strains that have been genetically modified and/or grown under conditions that favor the over production of fatty acids. The successful candidate will be able to perform basic and advanced bioimaging protocols such as: confocal microscopy, cryofixation and freeze substitution, ultramicrotomy, standard TEM and SEM imaging and electron tomography, and immuno-cytochemistry at both the light and EM levels. The School of Life Sciences Bioimaging Facility (http://sols.asu.edu/klab/index.php; http://sols.asu.edu/lsem/index.php) and associated imaging facilities at Arizona State University maintain the equipment required to fulfill the goals of the project.
Contact: Dr. Robert Roberson School of Life Sciences PO Box874501 Arizona State University Tempe, AZ 85287-4501
I've used different sized boroscopes before, like the ones at www.uxr.com (I have no affiliation with this company, I've just seen their products in action before)
I would say that a small boroscope would be your best bet, as you can port it out to video, and some come with a light source, but others may need a separate light source. I know they make both a flexible and a rigid boroscope, the rigid one being slightly less expensive.
What is the size diameter you are looking for?
--Justin A. Kraft
On 6/7/07, Rosemary.White-at-csiro.au {Rosemary.White-at-csiro.au} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } } Are there any really tiny cameras about, or at least, fibre-optic } lenses/objectives? I ask because we have a couple of applications that } could use one. We're interested in roots growing down pre-existing soil } pores, and would like to watch what they're doing down there, either with a } moving imaging device of some kind, or a series of portholes to watch roots } growing past. Another group is screening different lines of cereals, in } particular, looking at development of the floral meristem. This object of } desire is enclosed in many leaves at ground level in a cereal plant, we'd } like to poke a small fibre-optic in to watch its development over 3-6 days, } rather than having to destroy many plants to follow the stages of } development. } } I've had a rummage through numerous websites, and sent off some email } enquiries, but wondered if any on this list were aware of available } technology to do this. } } cheers, } Rosemary } } Dr Rosemary White rosemary.white-at-csiro.au } CSIRO Plant Industry ph. 61 (0)2-6246 5475 } GPO Box 1600 fax. 61 (0)2-6246 5334 } Canberra, ACT 2601 } Australia } } ==============================Original Headers============================== } 5, 22 -- From Rosemary.White-at-csiro.au Thu Jun 7 18:06:13 2007 } 5, 22 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au [150.229.7.37]) } 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57N6BUp006428 } 5, 22 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 18:06:12 -0500 } 5, 22 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=B/719hCOmj70x4fw8Xa9jlsNCiQ81UNL/KVQyRNlI1roNRlDs0bdsuAYvhHWzstYOtqiQHorAT8msXRgrzpp2byXutq6Cr6KK0FIId20OL2fZ/pEb92ipjpFWEHFSiHv; } 5, 22 -- X-IronPort-AV: E=Sophos;i="4.16,397,1175436000"; } 5, 22 -- d="scan'208";a="162041683" } 5, 22 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) } 5, 22 -- by act-ironport-int.csiro.au with ESMTP; 08 Jun 2007 09:06:03 +1000 } 5, 22 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); } 5, 22 -- Fri, 8 Jun 2007 09:06:10 +1000 } 5, 22 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 } 5, 22 -- Date: Fri, 08 Jun 2007 09:09:44 +1000 } 5, 22 -- Subject: fibre-optic probes/cameras? } 5, 22 -- From: Rosemary White {Rosemary.White-at-csiro.au} } 5, 22 -- To: {microscopy-at-microscopy.com} } 5, 22 -- Message-ID: {C28ECD58.1DFA3%Rosemary.White-at-csiro.au} } 5, 22 -- In-Reply-To: {200705311420.l4VEKqBb024233-at-ns.microscopy.com} } 5, 22 -- Mime-version: 1.0 } 5, 22 -- Content-type: text/plain; charset="US-ASCII" } 5, 22 -- Content-transfer-encoding: 7bit } 5, 22 -- X-OriginalArrivalTime: 07 Jun 2007 23:06:10.0382 (UTC) FILETIME=[6FF3E6E0:01C7A958] } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 29 -- From kraftpiano-at-gmail.com Thu Jun 7 18:43:38 2007 5, 29 -- Received: from wa-out-1112.google.com (wa-out-1112.google.com [209.85.146.179]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57NhbbJ029818 5, 29 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 18:43:37 -0500 5, 29 -- Received: by wa-out-1112.google.com with SMTP id v27so873009wah 5, 29 -- for {microscopy-at-microscopy.com} ; Thu, 07 Jun 2007 16:43:37 -0700 (PDT) 5, 29 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 5, 29 -- d=gmail.com; s=beta; 5, 29 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:cc:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 5, 29 -- b=ZMktC4Ijr/TBvOgIWjQAwJtSx5txDM8jd8jch/gnr6PixoqAo9MxT/HL8wsdtBREW+HSIMUO1B62JAhJJE2MEXT/El98Eq55eghVFW+D2wFYqf4+kx9GMqs0MEfWugYn2FUjfYFtt66VvovSuc0km0RBRBZPhBp0FuppKgYHOtI= 5, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 29 -- d=gmail.com; s=beta; 5, 29 -- h=received:message-id:date:from:to:subject:cc:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 5, 29 -- b=hPC2cFzenkRaqHdnSplzfRJ2/lHeMsTcOrM6KlsibZeA4nJutLKl6UI1vBhNYsrdPr1xBkE/byUR9jF8uGryKUteGA45HIK/ZLJd73wxQ70m8WVSc3P6NS2RXFUTXUQ0H4xFAtCBvYZEw38j3ADfQeUTSif61fd6nACH++3HroU= 5, 29 -- Received: by 10.115.90.1 with SMTP id s1mr2016369wal.1181259816159; 5, 29 -- Thu, 07 Jun 2007 16:43:36 -0700 (PDT) 5, 29 -- Received: by 10.114.92.13 with HTTP; Thu, 7 Jun 2007 16:43:36 -0700 (PDT) 5, 29 -- Message-ID: {25e2b0d20706071643l4e733e0fse755981ee390bf4-at-mail.gmail.com} 5, 29 -- Date: Thu, 7 Jun 2007 19:43:36 -0400 5, 29 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 5, 29 -- To: Rosemary.White-at-csiro.au 5, 29 -- Subject: Re: [Microscopy] fibre-optic probes/cameras? 5, 29 -- Cc: microscopy-at-microscopy.com 5, 29 -- In-Reply-To: {200706072314.l57NEo81017021-at-ns.microscopy.com} 5, 29 -- MIME-Version: 1.0 5, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 29 -- Content-Transfer-Encoding: 7bit 5, 29 -- Content-Disposition: inline 5, 29 -- References: {200706072314.l57NEo81017021-at-ns.microscopy.com} ==============================End of - Headers==============================
Hi Rosemary! Have you contacted Optiscan? They have some pretty small endoscopic microscopy devices which are really powerful. I'm sure they'd be as good for your application as they are for animal work. www.optiscan.com
Cheers, Roseys
I have no affiliation with Optiscan, just impressed by the demos I've seen
Rosemary van Driel Electron Microscopy New and Emerging Zoonotic Diseases Australian Animal Health Laboratory CSIRO Livestock Industries Private Bag 24 Geelong Vic 3220 Australia
International Phone: +61 3 5227 5209 International Fax: +61 3 5227 5555 email: Rosey.VanDriel-at-csiro.au
-----Original Message----- X-from: White, Rosemary (PI, Black Mountain) Sent: Friday, 8 June 2007 09:08 To: Van Driel, Rosey (LI, Geelong)
Dear all,
Are there any really tiny cameras about, or at least, fibre-optic lenses/objectives? I ask because we have a couple of applications that could use one. We're interested in roots growing down pre-existing soil pores, and would like to watch what they're doing down there, either with a moving imaging device of some kind, or a series of portholes to watch roots growing past. Another group is screening different lines of cereals, in particular, looking at development of the floral meristem. This object of desire is enclosed in many leaves at ground level in a cereal plant, we'd like to poke a small fibre-optic in to watch its development over 3-6 days, rather than having to destroy many plants to follow the stages of development.
I've had a rummage through numerous websites, and sent off some email enquiries, but wondered if any on this list were aware of available technology to do this.
cheers, Rosemary
Dr Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 61 (0)2-6246 5475 GPO Box 1600 fax. 61 (0)2-6246 5334 Canberra, ACT 2601 Australia
==============================Original Headers============================== 5, 22 -- From Rosemary.White-at-csiro.au Thu Jun 7 18:06:13 2007 5, 22 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au [150.229.7.37]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57N6BUp006428 5, 22 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 18:06:12 -0500 5, 22 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=B/719hCOmj70x4fw8Xa9jlsNCiQ81UNL/KVQyRNlI1roNRlDs0bdsuAYvhHWzstYOtqiQH orAT8msXRgrzpp2byXutq6Cr6KK0FIId20OL2fZ/pEb92ipjpFWEHFSiHv; 5, 22 -- X-IronPort-AV: E=Sophos;i="4.16,397,1175436000"; 5, 22 -- d="scan'208";a="162041683" 5, 22 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) 5, 22 -- by act-ironport-int.csiro.au with ESMTP; 08 Jun 2007 09:06:03 +1000 5, 22 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 5, 22 -- Fri, 8 Jun 2007 09:06:10 +1000 5, 22 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 5, 22 -- Date: Fri, 08 Jun 2007 09:09:44 +1000 5, 22 -- Subject: fibre-optic probes/cameras? 5, 22 -- From: Rosemary White {Rosemary.White-at-csiro.au} 5, 22 -- To: {microscopy-at-microscopy.com} 5, 22 -- Message-ID: {C28ECD58.1DFA3%Rosemary.White-at-csiro.au} 5, 22 -- In-Reply-To: {200705311420.l4VEKqBb024233-at-ns.microscopy.com} 5, 22 -- Mime-version: 1.0 5, 22 -- Content-type: text/plain; charset="US-ASCII" 5, 22 -- Content-transfer-encoding: 7bit 5, 22 -- X-OriginalArrivalTime: 07 Jun 2007 23:06:10.0382 (UTC) FILETIME=[6FF3E6E0:01C7A958] ==============================End of - Headers==============================
==============================Original Headers============================== 22, 31 -- From Rosey.VanDriel-at-csiro.au Thu Jun 7 18:50:27 2007 22, 31 -- Received: from vic-MTAout6.csiro.au (vic-MTAout6.csiro.au [150.229.64.43]) 22, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57NoQc2008909 22, 31 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 18:50:26 -0500 22, 31 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=WfNS2mgwhJwqdlpxH1z4ftNNa/glfAqKsHgIBZlQyh3MJ2ueFFVCIOoozd9LtNrX1HVd7tc0UcpR42VtEgnkeir7XPncNrCP2N6dVrADO0CSQXo1wFLMTbE+3je8+KuU; 22, 31 -- X-IronPort-AV: E=Sophos;i="4.16,397,1175436000"; 22, 31 -- d="scan'208";a="137883350" 22, 31 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 22, 31 -- by vic-ironport-int.csiro.au with ESMTP; 08 Jun 2007 09:50:25 +1000 22, 31 -- Received: from exvicn1-mel.nexus.csiro.au ([138.194.3.60]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 22, 31 -- Fri, 8 Jun 2007 09:50:25 +1000 22, 31 -- Received: from EXVIC4-GEX.nexus.csiro.au ([138.194.208.10]) by exvicn1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 22, 31 -- Fri, 8 Jun 2007 09:50:25 +1000 22, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 22, 31 -- content-class: urn:content-classes:message 22, 31 -- MIME-Version: 1.0 22, 31 -- Content-Type: text/plain; 22, 31 -- charset="US-ASCII" 22, 31 -- Subject: RE: [Microscopy] fibre-optic probes/cameras? 22, 31 -- Date: Fri, 8 Jun 2007 09:49:08 +1000 22, 31 -- Message-ID: {8BE5C4878E8768478169A50D385160522E36BE-at-exvic4-gex.nexus.csiro.au} 22, 31 -- X-MS-Has-Attach: 22, 31 -- X-MS-TNEF-Correlator: 22, 31 -- Thread-Topic: [Microscopy] fibre-optic probes/cameras? 22, 31 -- Thread-Index: AcepWLV0lhqD+qj/RqmDOW5ZKM2bswABFxnQ 22, 31 -- References: {200706072308.l57N834Y009222-at-ns.microscopy.com} 22, 31 -- From: {Rosey.VanDriel-at-csiro.au} 22, 31 -- To: {Rosemary.White-at-csiro.au} , {microscopy-at-microscopy.com} 22, 31 -- X-OriginalArrivalTime: 07 Jun 2007 23:50:25.0038 (UTC) FILETIME=[9E4042E0:01C7A95E] 22, 31 -- Content-Transfer-Encoding: 8bit 22, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l57NoQc2008909 ==============================End of - Headers==============================
The diameter - as small as possible..... ideally 1mm or less, which I know is smaller than most catheter imaging systems, for example. However, for the root project, the boroscopes look pretty good. And of course, I got more web results using fiber-optics rather than fibre-optics.....the boroscope site didn¹t show up before... thanks, cheers, Rosemary
} From: "Justin Kraft" {kraftpiano-at-gmail.com} } Date: Thu, 7 Jun 2007 19:43:36 -0400 } To: Rosemary.White-at-csiro.au } Cc: microscopy-at-microscopy.com } Subject: Re: [Microscopy] fibre-optic probes/cameras? } } I've used different sized boroscopes before, like the ones at } www.uxr.com (I have no affiliation with this company, I've just seen } their products in action before) } } I would say that a small boroscope would be your best bet, as you can } port it out to video, and some come with a light source, but others } may need a separate light source. I know they make both a flexible } and a rigid boroscope, the rigid one being slightly less expensive. } } What is the size diameter you are looking for? } } --Justin A. Kraft } } On 6/7/07, Rosemary.White-at-csiro.au {Rosemary.White-at-csiro.au} wrote: } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear all, } } } } Are there any really tiny cameras about, or at least, fibre-optic } } lenses/objectives? I ask because we have a couple of applications that } } could use one. We're interested in roots growing down pre-existing soil } } pores, and would like to watch what they're doing down there, either with a } } moving imaging device of some kind, or a series of portholes to watch roots } } growing past. Another group is screening different lines of cereals, in } } particular, looking at development of the floral meristem. This object of } } desire is enclosed in many leaves at ground level in a cereal plant, we'd } } like to poke a small fibre-optic in to watch its development over 3-6 days, } } rather than having to destroy many plants to follow the stages of } } development. } } } } I've had a rummage through numerous websites, and sent off some email } } enquiries, but wondered if any on this list were aware of available } } technology to do this. } } } } cheers, } } Rosemary } } } } Dr Rosemary White rosemary.white-at-csiro.au } } CSIRO Plant Industry ph. 61 (0)2-6246 5475 } } GPO Box 1600 fax. 61 (0)2-6246 5334 } } Canberra, ACT 2601 } } Australia } } } } ==============================Original Headers============================== } } 5, 22 -- From Rosemary.White-at-csiro.au Thu Jun 7 18:06:13 2007 } } 5, 22 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au } } [150.229.7.37]) } } 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } l57N6BUp006428 } } 5, 22 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 18:06:12 } } -0500 } } 5, 22 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; } } b=B/719hCOmj70x4fw8Xa9jlsNCiQ81UNL/KVQyRNlI1roNRlDs0bdsuAYvhHWzstYOtqiQHorAT8 } } msXRgrzpp2byXutq6Cr6KK0FIId20OL2fZ/pEb92ipjpFWEHFSiHv; } } 5, 22 -- X-IronPort-AV: E=Sophos;i="4.16,397,1175436000"; } } 5, 22 -- d="scan'208";a="162041683" } } 5, 22 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) } } 5, 22 -- by act-ironport-int.csiro.au with ESMTP; 08 Jun 2007 09:06:03 } } +1000 } } 5, 22 -- Received: from [152.83.167.45] ([152.83.167.45]) by } } exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); } } 5, 22 -- Fri, 8 Jun 2007 09:06:10 +1000 } } 5, 22 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 } } 5, 22 -- Date: Fri, 08 Jun 2007 09:09:44 +1000 } } 5, 22 -- Subject: fibre-optic probes/cameras? } } 5, 22 -- From: Rosemary White {Rosemary.White-at-csiro.au} } } 5, 22 -- To: {microscopy-at-microscopy.com} } } 5, 22 -- Message-ID: {C28ECD58.1DFA3%Rosemary.White-at-csiro.au} } } 5, 22 -- In-Reply-To: {200705311420.l4VEKqBb024233-at-ns.microscopy.com} } } 5, 22 -- Mime-version: 1.0 } } 5, 22 -- Content-type: text/plain; charset="US-ASCII" } } 5, 22 -- Content-transfer-encoding: 7bit } } 5, 22 -- X-OriginalArrivalTime: 07 Jun 2007 23:06:10.0382 (UTC) } } FILETIME=[6FF3E6E0:01C7A958] } } ==============================End of - Headers============================== } } }
==============================Original Headers============================== 3, 26 -- From Rosemary.White-at-csiro.au Thu Jun 7 19:22:22 2007 3, 26 -- Received: from act-MTAout6.csiro.au (act-MTAout6.csiro.au [150.229.7.43]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l580MLwT020803 3, 26 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 19:22:21 -0500 3, 26 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=Sd9ytDUi7g/wtl0fwJPRyW5eLlwis3Fnc9taQvk1kgIW9PEahRmbB5TMEfJT/JOt9NhcKnif65t3ytsp/1dqedCjyUVwy9PIQ9sArUnnGha5Yi1Kb9RoqwgZ/F/iUP9T; 3, 26 -- X-IronPort-AV: E=Sophos;i="4.16,397,1175436000"; 3, 26 -- d="scan'208";a="162049929" 3, 26 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 3, 26 -- by act-ironport-int.csiro.au with ESMTP; 08 Jun 2007 10:22:11 +1000 3, 26 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 3, 26 -- Fri, 8 Jun 2007 10:22:14 +1000 3, 26 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 3, 26 -- Fri, 8 Jun 2007 10:22:14 +1000 3, 26 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 3, 26 -- Date: Fri, 08 Jun 2007 10:25:48 +1000 3, 26 -- Subject: Re: [Microscopy] fibre-optic probes/cameras? 3, 26 -- From: Rosemary White {Rosemary.White-at-csiro.au} 3, 26 -- To: Justin Kraft {kraftpiano-at-gmail.com} 3, 26 -- CC: {microscopy-at-microscopy.com} 3, 26 -- Message-ID: {C28EDF2C.1DFC9%Rosemary.White-at-csiro.au} 3, 26 -- In-Reply-To: {25e2b0d20706071643l4e733e0fse755981ee390bf4-at-mail.gmail.com} 3, 26 -- Mime-version: 1.0 3, 26 -- Content-type: text/plain; charset="ISO-8859-1" 3, 26 -- X-OriginalArrivalTime: 08 Jun 2007 00:22:14.0290 (UTC) FILETIME=[1040FF20:01C7A963] 3, 26 -- Content-Transfer-Encoding: 8bit 3, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l580MLwT020803 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tstrixnerharvey-at-qmag.com.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Purchasing an entry level SEM
Question: Good Morning Everyone,
I have been given the task of purchasing an "entry level" SEM capable of doing surface analaysis and elemental mapping of mineralogical samples.
Unfortunately may knowledge of SEM extends to sending samples away to a university and getting beautiful reports and photographs back.
What I need is an instrument able to do EDS measurements of light elements Z=11 and above. My samples need to be carbon coated and have an approximate minimum crystal size of 5 micron.
I want to use the instrument to look at gross crystal structures (at 5 micron) such as nicely layered or random etc.
Also I want to do elemental mapping of different products at larger crystal sizes (30-5000um).
I have a reasonable budget but not extensive.
I would appreciate some pointers.
Thank you kindly.
Kind Regards,
Tania Strixner-Harvey Principal - Research and Development
Queensland Magnesia 246 Boundary Road Parkhurst, 4702 Australia
X-from what you describe, you need not an "entry level" SEM, but a conventional SEM (high vacuum, W filament) and a reasonable EDS with high count rate and software to be able to do mapping.
I have JEOL 6400 with Oxford INCA EDS, it comfortably does what you describe. The only complication is that the largest map it can do at a time is about 6 mm. So, to acquire an elemental map of 5 mm crystals and have several of them in the field of view you will also need a motorised stage controlled by the EDS software. An alternative would be to collect several maps and stitch them together.
You will also need to budget for some cutting/polishing equipment a carbon coater.
Alex
============== Alexander Titkov Senior Scientist Millennium Inorganic Chemicals a Cristal Company Lot 4 Old Coast Road Australind WA 6233 AUSTRALIA
tstrixnerharvey-at-q mag.com.au To: alex.titkov-at-millenniumchem.com cc: 08/06/2007 08:38 Subject: [Microscopy] viaWWW: Purchasing an entry level SEM AM Please respond to tstrixnerharvey
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tstrixnerharvey-at-qmag.com.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Purchasing an entry level SEM
Question: Good Morning Everyone,
I have been given the task of purchasing an "entry level" SEM capable of doing surface analaysis and elemental mapping of mineralogical samples.
Unfortunately may knowledge of SEM extends to sending samples away to a university and getting beautiful reports and photographs back.
What I need is an instrument able to do EDS measurements of light elements Z=11 and above. My samples need to be carbon coated and have an approximate minimum crystal size of 5 micron.
I want to use the instrument to look at gross crystal structures (at 5 micron) such as nicely layered or random etc.
Also I want to do elemental mapping of different products at larger crystal sizes (30-5000um).
I have a reasonable budget but not extensive.
I would appreciate some pointers.
Thank you kindly.
Kind Regards,
Tania Strixner-Harvey Principal - Research and Development
Queensland Magnesia 246 Boundary Road Parkhurst, 4702 Australia
==============================Original Headers ============================== 16, 11 -- From zaluzec-at-microscopy.com Thu Jun 7 19:36:01 2007 16, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 16, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l580Zx4V032694 16, 11 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 19:36:00 -0500 16, 11 -- Mime-Version: 1.0 16, 11 -- Message-Id: {p06240802c28e54de9ab7-at-[206.69.208.22]} 16, 11 -- Date: Thu, 7 Jun 2007 19:35:59 -0500 16, 11 -- To: microscopy-at-microscopy.com 16, 11 -- From: tstrixnerharvey-at-qmag.com.au (by way of MicroscopyListserver) 16, 11 -- Subject: viaWWW: Purchasing an entry level SEM 16, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers ==============================
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==============================Original Headers============================== 49, 23 -- From alex.titkov-at-millenniumchem.com Thu Jun 7 20:36:53 2007 49, 23 -- Received: from edcpmp01.lyondell.com (mail4.lyondell.com [161.16.0.78]) 49, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l581ar9f012984 49, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 20:36:53 -0500 49, 23 -- Received: from edcexp01.lyondell.com ([161.16.151.68]) 49, 23 -- by edcpap02.lyondell.com (8.13.7/8.13.7) with ESMTP id l581aqG3020793; 49, 23 -- Thu, 7 Jun 2007 20:36:52 -0500 49, 23 -- Received: from amnhhtv01.millenniumchem.com ([172.19.0.46]) by edcexp01.lyondell.com with Microsoft SMTPSVC(5.0.2195.6713); 49, 23 -- Thu, 7 Jun 2007 20:36:51 -0500 49, 23 -- Subject: Re: [Microscopy] viaWWW: Purchasing an entry level SEM 49, 23 -- To: tstrixnerharvey-at-qmag.com.au 49, 23 -- Cc: Microscopy-at-microscopy.com 49, 23 -- X-Mailer: Lotus Notes Release 5.0.11 July 24, 2002 49, 23 -- Message-ID: {OFAC77D30E.868B4AF1-ONC82572F4.000639C3-C82572F4.0008BBDD-at-millenniumchem.com} 49, 23 -- From: alex.titkov-at-millenniumchem.com 49, 23 -- Date: Fri, 8 Jun 2007 10:35:14 +0900 49, 23 -- X-MIMETrack: Serialize by Router on AMNHHTV01/HUB/SRV/MIC(Release 5.0.11 |July 24, 2002) at 49, 23 -- 06/07/2007 09:36:51 PM 49, 23 -- MIME-Version: 1.0 49, 23 -- Content-type: text/plain; charset=us-ascii 49, 23 -- X-OriginalArrivalTime: 08 Jun 2007 01:36:51.0757 (UTC) FILETIME=[7D083DD0:01C7A96D] 49, 23 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=3.1.0-0705030000 definitions=main-0706070102 49, 23 -- X-Proofpoint-OriginalSender: alex.titkov-at-millenniumchem.com ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (ardnek2-at-att.net ) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, June 7, 2007 at 21:03:50 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both ardnek2-at-att.net as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: ardnek2-at-att.net Name: Kendra Orr
Organization: ARDNEK TUTORING
Education: 6-8th Grade Middle School
Location: Fort Lauderdale, FL 33334
Title: Re: Syringes with spores
Question: This may seem like a stupid question. However, I want to be precise. May I order a spore syringe and it will spit out spores tiny enough to see on a microscope? I am sorry........I am doing a presentation and I usually use spores from real plants that I cultivate.
Do you or anyone else know if a large fiber optic strand of .25 to 1.0 mm can conduct an image that can be viewed with am 20x to 50x microscope objective on the polished end of the fiber away form the subject in this case roots.
I believe a lens could melted on the other end of a single fiber and computer software could correct the distortions.
In a project such are yours I would think having a great number of low cost lenses wold give much better coverage and allow you to cut years of the time it takes to get results.
Gordon Couger Stillwater OK 405 624 2855 Rosemary.White-at-csiro.au wrote: } } The diameter - as small as possible..... ideally 1mm or less, which I know } is smaller than most catheter imaging systems, for example. However, for } the root project, the boroscopes look pretty good. } And of course, I got more web results using fiber-optics rather than } fibre-optics.....the boroscope site didn¹t show up before... } thanks, } cheers, } Rosemary } } } } From: "Justin Kraft" {kraftpiano-at-gmail.com} } } Date: Thu, 7 Jun 2007 19:43:36 -0400 } } To: Rosemary.White-at-csiro.au } } Cc: microscopy-at-microscopy.com } } Subject: Re: [Microscopy] fibre-optic probes/cameras? } } } } I've used different sized boroscopes before, like the ones at } } www.uxr.com (I have no affiliation with this company, I've just seen } } their products in action before) } } } } I would say that a small boroscope would be your best bet, as you can } } port it out to video, and some come with a light source, but others } } may need a separate light source. I know they make both a flexible } } and a rigid boroscope, the rigid one being slightly less expensive. } } } } What is the size diameter you are looking for? } } } } --Justin A. Kraft } } } } On 6/7/07, Rosemary.White-at-csiro.au {Rosemary.White-at-csiro.au} wrote: } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Dear all, } } } } } } Are there any really tiny cameras about, or at least, fibre-optic } } } lenses/objectives? I ask because we have a couple of applications that } } } could use one. We're interested in roots growing down pre-existing soil } } } pores, and would like to watch what they're doing down there, either with a } } } moving imaging device of some kind, or a series of portholes to watch roots } } } growing past. Another group is screening different lines of cereals, in } } } particular, looking at development of the floral meristem. This object of } } } desire is enclosed in many leaves at ground level in a cereal plant, we'd } } } like to poke a small fibre-optic in to watch its development over 3-6 days, } } } rather than having to destroy many plants to follow the stages of } } } development. } } } } } } I've had a rummage through numerous websites, and sent off some email } } } enquiries, but wondered if any on this list were aware of available } } } technology to do this. } } } } } } cheers, } } } Rosemary } } } } } } Dr Rosemary White rosemary.white-at-csiro.au } } } CSIRO Plant Industry ph. 61 (0)2-6246 5475 } } } GPO Box 1600 fax. 61 (0)2-6246 5334 } } } Canberra, ACT 2601 } } } Australia }
==============================Original Headers============================== 8, 20 -- From gcouger-at-science-info.net Fri Jun 8 02:50:52 2007 8, 20 -- Received: from smtp106.biz.mail.re2.yahoo.com (smtp106.biz.mail.re2.yahoo.com [206.190.52.175]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l587opaR009827 8, 20 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jun 2007 02:50:51 -0500 8, 20 -- Received: (qmail 30482 invoked from network); 8 Jun 2007 07:50:51 -0000 8, 20 -- Received: from unknown (HELO ?192.168.1.100?) (gcouger-at-science-info.net-at-74.195.225.38 with plain) 8, 20 -- by smtp106.biz.mail.re2.yahoo.com with SMTP; 8 Jun 2007 07:50:50 -0000 8, 20 -- X-YMail-OSG: YzK7UdQVM1kexYzHUc3hxlL1LTVHQrZshdzxUN5jleVa_xvSqaiVD0pV4gmheHGNHLKJ2kmKz6gAZvF_p1GuvTeiSn_bctFCdeVoI2R_areKwJxhkL15O4VbmIxwcZV6vJSinAA0jydeaw-- 8, 20 -- Message-ID: {46690A5F.3030508-at-science-info.net} 8, 20 -- Date: Fri, 08 Jun 2007 02:50:55 -0500 8, 20 -- From: Gordon Couger {gcouger-at-science-info.net} 8, 20 -- User-Agent: Thunderbird 1.5.0.12 (Macintosh/20070509) 8, 20 -- MIME-Version: 1.0 8, 20 -- To: Rosemary.White-at-csiro.au, microscopy-at-microscopy.com 8, 20 -- Subject: Re: [Microscopy] Re: fibre-optic probes/cameras? 8, 20 -- References: {200706080027.l580RXK4031395-at-ns.microscopy.com} 8, 20 -- In-Reply-To: {200706080027.l580RXK4031395-at-ns.microscopy.com} 8, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l587opaR009827 ==============================End of - Headers==============================
Been not involved in carbon nanotube research I dont know exactly how dense they are under the beam of a TEM. However I don't think you can compare the density of the carbon present in a tissue with the density of carbon in nanotubes. The difference MUST be visible. I wouldn't modify the nanotubes themselves because you will definitely modify their behaviour in an uncontrolled manner. Now here is what I would do: I would incubate cell monolayers with the nanoparticles and flat embed them (control=cells incubated at 4°C, no uptake) after ferrocyanide-osmium post-fixation. I am pretty sure the cells will internalize the particles (take hepatocytes like HepG2 they are very effecient at uptaking and very nice to show). After sectionning, I would try different contrasting intensities and also without any contrasting at all! This way you can define your technical parameters to obtain the best contrast possible in "control" samples.
Finding these particles in organs is another story, requiring mainly eternities of patience and dedication. But, if I may give my personal opinion, this is a very interesting study and probably a very rewarding one. Few have had the heart to start it.
Regards and good luck,
Stephane
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==============================Original Headers============================== 9, 21 -- From nizets2-at-yahoo.com Fri Jun 8 05:52:37 2007 9, 21 -- Received: from web37414.mail.mud.yahoo.com (web37414.mail.mud.yahoo.com [209.191.91.146]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l58AqbJR028188 9, 21 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jun 2007 05:52:37 -0500 9, 21 -- Received: (qmail 36206 invoked by uid 60001); 8 Jun 2007 10:52:36 -0000 9, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 21 -- s=s1024; d=yahoo.com; 9, 21 -- h=X-YMail-OSG:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 9, 21 -- b=Th7IcHvEcm38JIhX3/l26JoxXFwymDMC5uDRN8g0oDgryha87yhpMfJ/YBB3aZl53Kro1J40P1SnNaaTGMit9wjcHw7nvSn5v5BIxymLkWVXs5jyL7V8hErX+l/+MtxBhwe06vj+BH2EZGakbfTnLz31RCHy/vxSBATSehs5b3I=; 9, 21 -- X-YMail-OSG: qHwdNQIVM1lbwnLKYcwm.jWyeXsF73stJh0XXkQWKZ6kiL20PdHKKfrhkFYFI4QjxALnY19L6V.hfcebfLhRZ5yi9pXY0oEjSCUlvJ3o7Ruc7M6H3ns- 9, 21 -- Received: from [80.122.101.102] by web37414.mail.mud.yahoo.com via HTTP; Fri, 08 Jun 2007 03:52:36 PDT 9, 21 -- Date: Fri, 8 Jun 2007 03:52:36 -0700 (PDT) 9, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 9, 21 -- Subject: Re: [Microscopy] RE: Carbon nanos in tissue: SEM/TEM 9, 21 -- To: TindallR-at-missouri.edu 9, 21 -- Cc: microscopy-at-microscopy.com 9, 21 -- In-Reply-To: {200706071700.l57H0ZUv016579-at-ns.microscopy.com} 9, 21 -- MIME-Version: 1.0 9, 21 -- Content-Type: text/plain; charset=iso-8859-1 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- Message-ID: {674274.31149.qm-at-web37414.mail.mud.yahoo.com} ==============================End of - Headers==============================
Dear Tania, You should consider a basic variable-pressure SEM. They are very good for mineralogical work and have the added bonus of not requiring a carbon coating. For what you want to do, the new, simple table-top SEMs, such as the Hitachi TM-1000 or the FEI Phenom-Ed might be ideal, if they do EDS. They are very fast, simple and low cost. They use BSE imaging, which shows up the grains on polished minerals very well. Otherwise , a simple, low-cost variable-pressure SEM will be your best bet. I didn't think that the variable-pressure SEM's had much to offer materials engineering applications, but we are using it all the time and finding new uses for it every day. BTW, all the modern EDS systems will do the elements Z=5 (boron) and above. Regards,
-----Original Message----- X-from: tstrixnerharvey-at-qmag.com.au [mailto:tstrixnerharvey-at-qmag.com.au] Sent: June 7, 2007 5:40 PM To: mager-at-interchange.ubc.ca
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Title-Subject: [Filtered] Purchasing an entry level SEM
Question: Good Morning Everyone,
I have been given the task of purchasing an "entry level" SEM capable of doing surface analaysis and elemental mapping of mineralogical samples.
Unfortunately may knowledge of SEM extends to sending samples away to a university and getting beautiful reports and photographs back.
What I need is an instrument able to do EDS measurements of light elements Z=11 and above. My samples need to be carbon coated and have an approximate minimum crystal size of 5 micron.
I want to use the instrument to look at gross crystal structures (at 5 micron) such as nicely layered or random etc.
Also I want to do elemental mapping of different products at larger crystal sizes (30-5000um).
I have a reasonable budget but not extensive.
I would appreciate some pointers.
Thank you kindly.
Kind Regards,
Tania Strixner-Harvey Principal - Research and Development
Queensland Magnesia 246 Boundary Road Parkhurst, 4702 Australia
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Question: I have a Cambridge SEM and I am trying to preform voltage contrast on a multi-layer ceramic capacitor. It has been well over 10 years since I've used VC and I must have forgotten something. I can blow them up, but can get them to light up. Does anyone have a guess on what I'm doing wrong. My parameters are 5 acceleration voltage. Power supply positive wire connected capacitor and negative connected to ground. SEM bias turned off. Thanks for any suggestions.
The May Archives for the Microscopy Listserver are now on-line at
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==============================Original Headers============================== 8, 11 -- From zaluzec-at-microscopy.com Sat Jun 9 11:49:38 2007 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l59Gnbxw012334 8, 11 -- for {microscopy-at-microscopy.com} ; Sat, 9 Jun 2007 11:49:38 -0500 8, 11 -- Mime-Version: 1.0 8, 11 -- Message-Id: {p0624080bc2908996a6f3-at-[206.69.208.22]} 8, 11 -- Date: Sat, 9 Jun 2007 11:49:37 -0500 8, 11 -- To: microscopy-at-microscopy.com 8, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 8, 11 -- Subject: Administrivia: May 2007 Archives on-line 8, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Johnson Matthey PLC is a specialist chemicals company focused on its core skills in catalysts, precious metals and fine chemicals. The Technology Centre, based at Sonning Common (approximately 40 miles West of London), undertakes research work for the group.
A vacancy has arisen at the Technology Centre for an Electron Microscopist to join our team working with both Scanning and Transmission microscopes.
The successful candidate is expected to have experience in several of the following areas of expertise:
SEM and TEM sample preparation: (Coating; Ultramicrotomy; Cryo-sample preparation) Electron Microscope technical knowledge: (FEG sources, vacuum systems; electronics; fault finding and diagnosis) SEM and TEM Characterisation methods: (HRTEM; STEM; HAADF Imaging; EDX; EELS; Electron diffraction analysis)
The job is based on the analysis of a variety of samples from our research groups and operating divisions, thus prior exposure to multidisciplinary subjects as well as catalysis and materials would be an advantage.
The successful candidate will be educated to HNC/Degree level as a minimum. Applications must be made in writing with full CV and current salary details to: Georgie Floyd, Personnel Officer, Johnson Matthey Technology Centre, Blounts Court, Sonning Common, Reading, RG4 9NH or e-mail hrjmtc-at-matthey.com
Closing date for applications: Friday 6th July 2007
If the reader of this email is not the intended recipient(s), please be advised that any dissemination, distribution or copying of this information is strictly prohibited. Johnson Matthey PLC has its main place of business at 40-42 Hatton Garden, London (020 7269 8400).
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Please note that your communication may be monitored in accordance with Johnson Matthey internal policy documentation.
==============================Original Headers============================== 18, 24 -- From goodlg-at-matthey.com Mon Jun 11 04:39:39 2007 18, 24 -- Received: from cluster-e.mailcontrol.com (cluster-e.mailcontrol.com [217.79.216.190]) 18, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5B9ddvn022539 18, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 04:39:39 -0500 18, 24 -- Received: from royhosmtp.pmp.royston.matthey.com ([194.202.191.212]) 18, 24 -- by rly08e.srv.mailcontrol.com (MailControl) with ESMTP id l5B9dYCc006082 18, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 10:39:35 +0100 18, 24 -- Received: from tsqhof6.trafalgar.jm ([192.168.1.238]) 18, 24 -- by royhosmtp.pmp.royston.matthey.com (8.13.6/8.13.6) with ESMTP id l5B9o7el009946 18, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 10:50:07 +0100 18, 24 -- Received: from London-MTA by tsqhof6.trafalgar.jm 18, 24 -- with Novell_GroupWise; Mon, 11 Jun 2007 10:39:34 +0100 18, 24 -- Message-Id: {s66d2666.019-at-tsqhof6.trafalgar.jm} 18, 24 -- X-Mailer: Novell GroupWise Internet Agent 6.5.4 18, 24 -- Date: Mon, 11 Jun 2007 10:38:59 +0100 18, 24 -- From: "Gregory Goodlet" {goodlg-at-matthey.com} 18, 24 -- To: {Microscopy-at-microscopy.com} 18, 24 -- Subject: SEM/TEM Position JM (U.K.) 18, 24 -- Mime-Version: 1.0 18, 24 -- Content-Type: text/plain; charset=US-ASCII 18, 24 -- Content-Disposition: inline 18, 24 -- X-Scanned-By: MailControl A-07-07-05 (www.mailcontrol.com) on 10.69.0.118 18, 24 -- Content-Transfer-Encoding: 8bit 18, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5B9ddvn022539 ==============================End of - Headers==============================
Hello all, I have been asked what the voltage and current conditions are for evaporation of aluminum in a tungsten basket in a vacuum evaporator. Is this sort of information available in a reference? I don't have much experience with metal evaporation, so any advice would be appreciated. Thanks, Kim
-- Kim Rensing Ph.D. Assistant Research Professor Manager, Microscopy and Imaging Facility
University of Calgary Health Sciences Centre B129 - 3330 Hospital Drive NW Calgary, AB, Canada T2N 4N1 403-220-3488 krensing-at-ucalgary.ca
==============================Original Headers============================== 4, 20 -- From krensing-at-ucalgary.ca Mon Jun 11 10:11:37 2007 4, 20 -- Received: from smtp3.ucalgary.ca (smtp3.ucalgary.ca [136.159.34.64]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5BFBbHO009211 4, 20 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 10:11:37 -0500 4, 20 -- Received: from [136.159.164.163] (unknown [136.159.164.163]) 4, 20 -- by smtp3.ucalgary.ca (Postfix) with ESMTP id 0AD5D4034 4, 20 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 09:11:31 -0600 (MDT) 4, 20 -- Message-ID: {466D661E.4080807-at-ucalgary.ca} 4, 20 -- Date: Mon, 11 Jun 2007 09:11:26 -0600 4, 20 -- From: Kim Rensing {krensing-at-ucalgary.ca} 4, 20 -- Organization: Microscopy and Imaging Facility, U. of Calgary 4, 20 -- User-Agent: Thunderbird 1.5.0.12 (Windows/20070509) 4, 20 -- MIME-Version: 1.0 4, 20 -- To: microscopy-at-microscopy.com 4, 20 -- Subject: aluminum evaporation 4, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 20 -- Content-Transfer-Encoding: 7bit 4, 20 -- X-UCalgary-MailScanner-Information: Please contact IT Help Desk at (403) 220-5555 for more information 4, 20 -- X-UCalgary-MailScanner: Found to be clean 4, 20 -- X-UCalgary-MailScanner-From: krensing-at-ucalgary.ca ==============================End of - Headers==============================
Can anyone recommend or otherwise provide information on an affordable(few thousand $) Peltier cold stage suitable for use in an SEM level vacuum (we will modify to fit several instruments)? If you know of suitable components, our machine shop can build or modify as needed. Thanks,
Dale Batchelor, Ph.D. Associate Director Analytical Instrumentation Facility N.C. State University Monteith Research Center room 318A Campus Box 7531 Raleigh, NC 27695 Office 919-515-3841 FAX 919-515-6965 E-Mail dale_batchelor-at-ncsu.edu Website www.ncsu.edu/aif
==============================Original Headers============================== 3, 19 -- From dale_batchelor-at-ncsu.edu Mon Jun 11 12:26:51 2007 3, 19 -- Received: from uni10mr.unity.ncsu.edu (uni10mr.unity.ncsu.edu [152.1.1.170]) 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5BHQpXn001010 3, 19 -- for {Microscopy-at-MSA.Microscopy.com} ; Mon, 11 Jun 2007 12:26:51 -0500 3, 19 -- Received: from [152.14.52.68] (dbmacx.aif.ncsu.edu [152.14.52.68]) 3, 19 -- by uni10mr.unity.ncsu.edu (8.13.7/8.13.8/Nv5.2006.1109) with ESMTP id l5BHQonb001347 3, 19 -- for {Microscopy-at-MSA.Microscopy.com} ; Mon, 11 Jun 2007 13:26:50 -0400 (EDT) 3, 19 -- Mime-Version: 1.0 (Apple Message framework v624) 3, 19 -- Content-Transfer-Encoding: 7bit 3, 19 -- Message-Id: {a0fb0d0d35f30cddf352e16f6bda9487-at-ncsu.edu} 3, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 3, 19 -- To: Microscopy-at-MSA.Microscopy.com 3, 19 -- From: Dale Batchelor {dale_batchelor-at-ncsu.edu} 3, 19 -- Subject: [Microscopy] SEM: Peltier Cooled Stage 3, 19 -- Date: Mon, 11 Jun 2007 13:28:21 -0400 3, 19 -- X-Mailer: Apple Mail (2.624) 3, 19 -- X-PMX-Version: 5.3.1.294258, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.6.11.100135 3, 19 -- X-Spam-Status: No, Hits=7% 3, 19 -- X-Spam-Level: IIIIIII ==============================End of - Headers==============================
Emitech has a Peltier Cold Stage for SEM, the K25X, however, you'll pay more than a few thousand dollars for the unit. Energy Beam Sciences is the US Master Distributor for Emitech Instruments, and we'd be happy to review your needs for this instrument with you.
Regards,
Mike Dufraine EM-Product Manager
dale_batchelor-at-ncsu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Can anyone recommend or otherwise provide information on an } affordable(few thousand $) Peltier cold stage suitable for use in an SEM } level vacuum (we will modify to fit several instruments)? If you know } of suitable components, our machine shop can build or modify as needed. } Thanks, } } Dale Batchelor, Ph.D. } Associate Director } Analytical Instrumentation Facility } N.C. State University } Monteith Research Center room 318A } Campus Box 7531 } Raleigh, NC 27695 } Office 919-515-3841 } FAX 919-515-6965 } E-Mail dale_batchelor-at-ncsu.edu } Website www.ncsu.edu/aif } } } ==============================Original Headers============================== } 3, 19 -- From dale_batchelor-at-ncsu.edu Mon Jun 11 12:26:51 2007 } 3, 19 -- Received: from uni10mr.unity.ncsu.edu (uni10mr.unity.ncsu.edu [152.1.1.170]) } 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5BHQpXn001010 } 3, 19 -- for {Microscopy-at-MSA.Microscopy.com} ; Mon, 11 Jun 2007 12:26:51 -0500 } 3, 19 -- Received: from [152.14.52.68] (dbmacx.aif.ncsu.edu [152.14.52.68]) } 3, 19 -- by uni10mr.unity.ncsu.edu (8.13.7/8.13.8/Nv5.2006.1109) with ESMTP id l5BHQonb001347 } 3, 19 -- for {Microscopy-at-MSA.Microscopy.com} ; Mon, 11 Jun 2007 13:26:50 -0400 (EDT) } 3, 19 -- Mime-Version: 1.0 (Apple Message framework v624) } 3, 19 -- Content-Transfer-Encoding: 7bit } 3, 19 -- Message-Id: {a0fb0d0d35f30cddf352e16f6bda9487-at-ncsu.edu} } 3, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed } 3, 19 -- To: Microscopy-at-MSA.Microscopy.com } 3, 19 -- From: Dale Batchelor {dale_batchelor-at-ncsu.edu} } 3, 19 -- Subject: [Microscopy] SEM: Peltier Cooled Stage } 3, 19 -- Date: Mon, 11 Jun 2007 13:28:21 -0400 } 3, 19 -- X-Mailer: Apple Mail (2.624) } 3, 19 -- X-PMX-Version: 5.3.1.294258, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.6.11.100135 } 3, 19 -- X-Spam-Status: No, Hits=7% } 3, 19 -- X-Spam-Level: IIIIIII } ==============================End of - Headers============================== }
-- Michael F. Dufraine EM - Product Manager TEL 800-992-9037 X340 MDufraine-at-ebsciences.com www.ebsciences.com
==============================Original Headers============================== 7, 26 -- From mdufraine-at-ebsciences.com Mon Jun 11 12:43:04 2007 7, 26 -- Received: from mx-outbound01.easydns.com (mailout.easydns.com [205.210.42.54]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5BHh3Sj012725 7, 26 -- for {Microscopy-at-MSA.Microscopy.com} ; Mon, 11 Jun 2007 12:43:03 -0500 7, 26 -- Received: from mail.ebsciences.com (unknown [69.182.224.2]) 7, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 7, 26 -- (No client certificate requested) 7, 26 -- by mx-outbound01.easydns.com (Postfix) with ESMTP id 225417FD5; 7, 26 -- Mon, 11 Jun 2007 13:43:00 -0400 (EDT) 7, 26 -- Received: from mdufraine.ebsciences.private ([10.10.0.196]) 7, 26 -- by mail.ebsciences.com with esmtpsa (TLSv1:AES256-SHA:256) 7, 26 -- (Exim 4.67) 7, 26 -- (envelope-from {mdufraine-at-ebsciences.com} ) 7, 26 -- id 1Hxnuu-0004yg-AO; Mon, 11 Jun 2007 13:43:00 -0400 7, 26 -- Message-ID: {466D89A3.6000904-at-ebsciences.com} 7, 26 -- Date: Mon, 11 Jun 2007 13:42:59 -0400 7, 26 -- From: Mike Dufraine {mdufraine-at-ebsciences.com} 7, 26 -- Organization: Energy Beam Sciences 7, 26 -- User-Agent: Thunderbird 2.0.0.0 (Windows/20070326) 7, 26 -- MIME-Version: 1.0 7, 26 -- To: dale_batchelor-at-ncsu.edu, Microscopy-at-MSA.Microscopy.com 7, 26 -- Subject: Re: [Microscopy] SEM: Peltier Cooled Stage 7, 26 -- References: {200706111727.l5BHRWl0002146-at-ns.microscopy.com} 7, 26 -- In-Reply-To: {200706111727.l5BHRWl0002146-at-ns.microscopy.com} 7, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I use a Deben XP stage and am very happy with it. The XP does extended temperature at high end which if not needed, would suggest a different model.
Deben is in the UK.
gary g.
At 09:28 AM 6/11/2007, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Mon Jun 11 13:34:35 2007 10, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5BIYZYl025891 10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 13:34:35 -0500 10, 20 -- Message-Id: {200706111834.l5BIYZYl025891-at-ns.microscopy.com} 10, 20 -- Received: (qmail 14333 invoked from network); 11 Jun 2007 11:34:34 -0700 10, 20 -- Received: by simscan 1.1.0 ppid: 14330, pid: 14331, t: 0.0845s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp1 with SMTP; 11 Jun 2007 11:34:34 -0700 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Mon, 11 Jun 2007 11:33:52 -0800 10, 20 -- To: dale_batchelor-at-ncsu.edu 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] SEM: Peltier Cooled Stage 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200706111728.l5BHSSKB003892-at-ns.microscopy.com} 10, 20 -- References: {200706111728.l5BHSSKB003892-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-1841652B ==============================End of - Headers==============================
Voltage and current requirements vary depending upon the volume of aluminum you are evaporating. We coat substrates in the Ladd evaporator by starting out with a very low voltage and adjusting it higher till the aluminum starts to evaporate.
If you wish you can call Mike Bouchard at Ladd - 1-800-451-3406 to discuss particulars. He has many years of experience in the manufacture of the Ladd evaporator and substrate coating.
John Arnott
Disclaimer: Ladd sells electron microscopy supplies including a vacuum evaporator, coated grids and substrates.
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==============================Original Headers============================== 13, 27 -- From jd-at-laddresearch.com Mon Jun 11 16:07:06 2007 13, 27 -- Received: from mclaren.electric.net (mclaren.electric.net [216.129.90.240]) 13, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5BL7514014822 13, 27 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 16:07:05 -0500 13, 27 -- Received: from root by mclaren.electric.net with emc1-ok (Exim 4.62) 13, 27 -- (envelope-from {jd-at-laddresearch.com} ) 13, 27 -- id 1Hxr6N-0001kd-Ux; Mon, 11 Jun 2007 14:07:03 -0700 13, 27 -- Received: by emcmailer; Mon, 11 Jun 2007 14:07:03 -0700 13, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 13, 27 -- by mclaren.electric.net with esmtps (TLSv1:AES256-SHA:256) 13, 27 -- (Exim 4.62) 13, 27 -- (envelope-from {jd-at-laddresearch.com} ) 13, 27 -- id 1Hxr6M-0001gj-Tc; Mon, 11 Jun 2007 14:07:02 -0700 13, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 27 -- Date: Mon, 11 Jun 2007 17:06:21 -0400 13, 27 -- To: krensing-at-ucalgary.ca 13, 27 -- From: jd {jd-at-laddresearch.com} 13, 27 -- Subject: Re: [Microscopy] aluminum evaporation 13, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 13, 27 -- In-Reply-To: {200706111518.l5BFITO6019591-at-ns.microscopy.com} 13, 27 -- References: {200706111518.l5BFITO6019591-at-ns.microscopy.com} 13, 27 -- Mime-Version: 1.0 13, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 13, 27 -- X-Outbound-IP: 216.204.198.170 13, 27 -- X-Env-From: jd-at-laddresearch.com 13, 27 -- X-Virus-Status: Scanned by VirusSMART (c) 13, 27 -- Message-Id: {E1Hxr6N-0001kd-Ux-at-mclaren.electric.net} ==============================End of - Headers==============================
FEI Company has a current opening for a Sr. Applications Engineer with a background in front and back side circuit edit. This position is located in Hillsboro, Oregon and would be responsible for providing equipment testing, acceptance, demonstration, training, marketing, and sales support to current and potential customers. If you are interested, please follow the link to see the entire job description. Feel free to apply directly online and I will see every resume that comes in. We also have several other openings listed on our website as well.
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==============================Original Headers============================== 8, 27 -- From Joe.Williamson-at-fei.com Mon Jun 11 16:28:37 2007 8, 27 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5BLSajC031233 8, 27 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Jun 2007 16:28:36 -0500 8, 27 -- X-WSS-ID: 0JJHQC3-01-3DZ-01 8, 27 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 8, 27 -- by smtp.feico.com (Tumbleweed MailGate) with ESMTP id 604F5DA8A29 8, 27 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Jun 2007 14:28:50 -0700 (PDT) 8, 27 -- Received: from hlexc03.w2k.feico.com ([10.150.40.39]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.1830); 8, 27 -- Mon, 11 Jun 2007 14:28:35 -0700 8, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 27 -- Content-class: urn:content-classes:message 8, 27 -- MIME-Version: 1.0 8, 27 -- Content-Type: text/plain; 8, 27 -- charset="us-ascii" 8, 27 -- Subject: FW: FEI Job Opportunity Circuit Edit Applications Engineer 8, 27 -- Date: Mon, 11 Jun 2007 14:28:41 -0700 8, 27 -- Message-ID: {00948E5E66F1374FB9284A7B78138EB80BA7C294-at-hlexc03.w2k.feico.com} 8, 27 -- X-MS-Has-Attach: 8, 27 -- X-MS-TNEF-Correlator: 8, 27 -- Thread-Topic: FEI Job Opportunity Circuit Edit Applications Engineer 8, 27 -- Thread-Index: Acep/XONVuukpaH7Rnu5qqfoLnfa4AAAE8fgAJxlSXA= 8, 27 -- From: "Williamson, Joe" {Joe.Williamson-at-fei.com} 8, 27 -- To: {Microscopy-at-Microscopy.Com} 8, 27 -- X-OriginalArrivalTime: 11 Jun 2007 21:28:35.0665 (UTC) FILETIME=[77EBFC10:01C7AC6F] 8, 27 -- Content-Transfer-Encoding: 8bit 8, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5BLSajC031233 ==============================End of - Headers==============================
Dear colleagues We would like to bring to your attention the symposium on Nanoscale Phenomena in Functional Materials by Scanning Probe Microscopy, to be held at the Materials Research Society 2007 Fall meeting in Boston, MA. The symposium description is attached below and can also be accessed on the MRS web-site (http://www.mrs.org/s_mrs/sec.asp?CID=8005&DID=192095). The deadline for abstract submission is June 20. Looking forward to seeing you in Boston On behalf of the organizers Sergei V. Kalinin
Symposium B: Nanoscale Phenomena in Functional Materials by Scanning Probe Microscopy
The last decade has witnessed spectacular progress in the development and applications of scanning probe microscopy (SPM)-based nanoscale imaging techniques. The combination of high spatial resolution and sensitivity to local electronic, optical, and mechanical properties places these techniques among the most versatile tools for nanoscience, biology, physics, and materials science. Atomic and electronic structure of surfaces, vibrational excitations, energy flow, and local materials properties on the molecular level has become accessible with the advent of high-resolution SPMs. Electrostatic SPMs are being established as powerful techniques for spatially resolved studies of electronic transport on the nanometer level at electroactive interfaces and in molecular electronic devices such as carbon nanotubes. Dynamic SPM modes and scanning indentation techniques allow mechanical compliance and surface energy to be investigated at the nanoscale with applications to the emerging fields of nanotribology, nanofluidics, nanocomposites, and NEMS. Novel optical imaging modes, such as tip-enhanced spectroscopy, solid immersion microscopy, and apertureless scanning optical microscopy, have joined the now-established near-field scanning optical microscopy (NSOM), bringing the resolution of optical spectroscopy into the nanoscale regime and complementing local electronic measurements of materials with the STM family of instruments. These new measurement techniques were necessitated by the growing need for materials characterization on the nanoscale and have in turn led to the discovery of new nanoscale phenomena. Finally, the SPM has been used to manipulate and fabricate materials at the nanoscale.
It is the goal of this symposium to provide a multidisciplinary forum for scanning-probe-based materials and nanoscience in order to demonstrate the latest achievements in technique developments and materials applications that have led to scientific discoveries. The symposium will include two types of sessions: One will be dedicated to the recent advances in technique development of interest to the materials community and will bring together specialists in practical and theoretical aspects of SPM imaging. The second will focus on specific materials-related phenomena, including nanotubes and nanowires, quantum dots, surfaces, interfaces, and biological systems studied by local probe techniques.
The topics of the symposium will include, but not be limited to:
* Imaging, manipulation, and energy transfer on the atomic and molecular level by atomic resolution NC-AFM and STM * Local optical and electronic properties and excitations, e.g., plasmons measured with SPMs * Defects, impurities, dopants, and transport in semiconductor nanostructures, nanotubes, and nanowires * Mechanics and electromechanics on the nanoscale by SPM and nanoindentation * Mechanical and voltage nanolithography and surface modification * Energy flows and dissipation in materials, devices, and nanostructures * Transport in single-molecule devices and carbon nanotubes * Imaging and characterization of ferroelectric materials * Electronic properties of semiconductor heterostructures * Imaging and characterization of biological systems * Dynamics and imaging of polymers and soft materials
Invited speakers include: *Robert Carpick* (Univ. of Pennsylvania), *Levent Degertekin* (Georgia Inst. of Technology), *Dennis Discher* (Univ. of Pennsylvania), *Ricardo Garcia* (Univ. Madrid, Spain), *Franz Giessibl* (Univ. Augsburg, Germany), *Venkat Gopalan* (Pennsylvania State Univ.), *Jan Hoh* (Johns Hopkins Univ.), *Ernesto Joselevich* (Weizmann Inst. of Science, Israel), *Maki Kawai* (RIKEN, Japan), *L. Kuipers* (FOM, The Netherlands), *Alexander Malkin* (Lawrence Livermore National Lab), *Lukas Novotny* (Univ. of Rochester), *E. Ward* *Plummer* (Univ. of Tennessee/Oak Ridge National Lab), *V. Sandoghdar* (ETH Zurich, Switzerland), *M. Tomitori* (JAIST, Japan), and *S. Wilks* (Swansea Univ., United Kingdom).
*Symposium Organizers*
*Dawn** Bonnell* University of Pennsylvania, Nano/Bio Interface Center, 3231 Walnut St., Philadelphia, PA 19104 Tel 215-898-6231, Fax 215-746-3204, bonnell-at-lrsm.upenn.edu {mailto:bonnell-at-lrsm.upenn.edu}
*Sergei V. Kalinin* Oak Ridge National Laboratory, Materials Sciences and Technology Division and Center for Nanophase Materials Sciences, 1 Bethel Valley Rd., Oak Ridge, TN 37831 Tel 865-241-0236, Fax 865-574-4143, sergei2-at-ornl.gov {mailto:sergei2-at-ornl.gov}
*Sidney R. Cohen* Weizmann Institute of Science, Surface Analysis Laboratory, Rehovot 76100 Israel Tel 972-8-934-2703/3422, Fax 972-8-934-4137, sidney.cohen-at-weizmann.ac.il {mailto:sidney.cohen-at-weizmann.ac.il}
*Richard E. Palmer* University of Birmingham, School of Physics and Astronomy, Nanoscale Physics Research Laboratory, Birmingham B15 2TT, United Kingdom Tel 44-121-414-4653, Fax 44-121-414-7327, r.e.palmer-at-bham.ac.uk {mailto:r.e.palmer-at-bham.ac.uk}
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This Question was submitted to Ask-A-Microscopist by (L.Ryves-at-physics.usyd.edu.au) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, June 11, 2007 at 09:13:35 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both L.Ryves-at-physics.usyd.edu.au as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: L.Ryves-at-physics.usyd.edu.au Name: Luke Ryves
Organization: School of Physics / University of Sydney
Education: Graduate College
Location: Sydney, NSW, Australia
Title: Beam voltage choice to minimise charging
Question: Hi,
I'm just writing up my thesis and want to include some SEM images that were acquired for me ages ago. The samples were metal particles on SiO2 surfaces. At the time that we were taking the images I remember the microscopist explaining that the correct energy (2 to 5kV on a FEG SEM) can reduce charging, but I can't find a reference to this effect. Can you help me out by explaining how to choose the beam energy, and perhaps point me in the direction of some references?
It is a fundamental principle that lower KV reduces charging. Joy, Goldstein and Newbury, et. al. have written about this factor. In practice, we see it all the time.
The actual KV value depends on the specimen and the ability of the SEM to image what you want to see. I use 500V to 2KV on SiO2 and Hf oxides. This is useful up to about 60KX with Zeiss Supra 55VP in HV mode. Other systems will likely differ.
Hope this helps.
gary g.
At 04:26 PM 6/11/2007, you wrote:
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==============================Original Headers============================== 11, 21 -- From gary-at-gaugler.com Mon Jun 11 19:45:15 2007 11, 21 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5C0jFL6008744 11, 21 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 19:45:15 -0500 11, 21 -- Message-Id: {200706120045.l5C0jFL6008744-at-ns.microscopy.com} 11, 21 -- Received: (qmail 22413 invoked from network); 11 Jun 2007 17:45:15 -0700 11, 21 -- Received: by simscan 1.1.0 ppid: 22410, pid: 22411, t: 0.0990s 11, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 21 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 21 -- by qsmtp4 with SMTP; 11 Jun 2007 17:45:15 -0700 11, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 21 -- Date: Mon, 11 Jun 2007 17:45:08 -0800 11, 21 -- To: L.Ryves-at-physics.usyd.edu.au 11, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 21 -- Subject: Re: [Microscopy] AskAMicroscopist: Beam voltage choice to 11, 21 -- minimise charging 11, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 21 -- In-Reply-To: {200706120026.l5C0Qc1i032230-at-ns.microscopy.com} 11, 21 -- References: {200706120026.l5C0Qc1i032230-at-ns.microscopy.com} 11, 21 -- Mime-Version: 1.0 11, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-AC03E08 ==============================End of - Headers==============================
There is no set voltage/current to evaporate aluminium. A variable power source is used to achieve this. A current is gradually increased until aluminium melts and then a little bit more increase starts the evaporation.
We achieved good results using a tungsten wire with v-shaped bend on which a small v-shaped piece of Al wire was hung.
Alex
============== Alexander Titkov Senior Scientist
Millennium Inorganic Chemicals a Cristal Company Locked Bag 245 Bunbury WA 6230 AUSTRALIA
krensing-at-ucalgary .ca To: alex.titkov-at-millenniumchem.com cc: 11/06/2007 11:15 Subject: [Microscopy] aluminum evaporation PM Please respond to krensing
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Hello all, I have been asked what the voltage and current conditions are for evaporation of aluminum in a tungsten basket in a vacuum evaporator. Is this sort of information available in a reference? I don't have much experience with metal evaporation, so any advice would be appreciated. Thanks, Kim
-- Kim Rensing Ph.D. Assistant Research Professor Manager, Microscopy and Imaging Facility
University of Calgary Health Sciences Centre B129 - 3330 Hospital Drive NW Calgary, AB, Canada T2N 4N1 403-220-3488 krensing-at-ucalgary.ca
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==============================Original Headers============================== 34, 23 -- From alex.titkov-at-millenniumchem.com Mon Jun 11 19:53:38 2007 34, 23 -- Received: from edcpap01.lyondell.com (mail3.lyondell.com [161.16.0.77]) 34, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5C0rcCd020330 34, 23 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 19:53:38 -0500 34, 23 -- Received: from edcexp01.lyondell.com ([161.16.151.68]) 34, 23 -- by edcpap01.lyondell.com (8.13.7/8.13.7) with ESMTP id l5C0rbW5009602; 34, 23 -- Mon, 11 Jun 2007 19:53:37 -0500 34, 23 -- Received: from amnhhtv01.millenniumchem.com ([172.19.0.46]) by edcexp01.lyondell.com with Microsoft SMTPSVC(5.0.2195.6713); 34, 23 -- Mon, 11 Jun 2007 19:53:37 -0500 34, 23 -- Subject: Re: [Microscopy] aluminum evaporation 34, 23 -- To: krensing-at-ucalgary.ca 34, 23 -- Cc: microscopy-at-microscopy.com 34, 23 -- X-Mailer: Lotus Notes Release 5.0.11 July 24, 2002 34, 23 -- Message-ID: {OFB5F0477B.737A4C2C-ONC82572F8.0002DCD6-C82572F8.0004C359-at-millenniumchem.com} 34, 23 -- From: alex.titkov-at-millenniumchem.com 34, 23 -- Date: Tue, 12 Jun 2007 09:51:51 +0900 34, 23 -- X-MIMETrack: Serialize by Router on AMNHHTV01/HUB/SRV/MIC(Release 5.0.11 |July 24, 2002) at 34, 23 -- 06/11/2007 08:53:37 PM 34, 23 -- MIME-Version: 1.0 34, 23 -- Content-type: text/plain; charset=us-ascii 34, 23 -- X-OriginalArrivalTime: 12 Jun 2007 00:53:37.0617 (UTC) FILETIME=[1C74E410:01C7AC8C] 34, 23 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=3.1.0-0705030000 definitions=main-0706110097 34, 23 -- X-Proofpoint-OriginalSender: alex.titkov-at-millenniumchem.com ==============================End of - Headers==============================
You can find information about varying the beam voltage in most any edition of "Scanning Electron Microscopy and X-Ray Microanalysis" (usually referred to as "SEMXM") by Goldstein, Newbury, Echlin, Joy, Fiori, and Lifshin. Look for information on the production efficiency of Secondary Electrons as a function of beam voltage. If I remember correctly, the SE coefficient went above 1 roughly between 800V and 1.5kV for SiO2 (anyone want to correct me?).
Cheers, Henk
At 08:26 PM 6/11/2007, you wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility (614) 292-0674 040 Fontana Labs, 116 W. 19th Ave www.ceof.ohio-state.edu
==============================Original Headers============================== 9, 27 -- From colijn.1-at-osu.edu Mon Jun 11 20:41:01 2007 9, 27 -- Received: from ER6S1.ENG.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5C1f1OS000377 9, 27 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 20:41:01 -0500 9, 27 -- Received: from CONVERSION-DAEMON.er6s1.eng.ohio-state.edu by 9, 27 -- er6s1.eng.ohio-state.edu (PMDF V6.2-1x11 #31056) 9, 27 -- id {01MHOEESUQ2OAAO8BG-at-er6s1.eng.ohio-state.edu} for 9, 27 -- microscopy-at-microscopy.com; Mon, 11 Jun 2007 21:41:00 -0400 (EDT) 9, 27 -- Received: from HOC3.osu.edu 9, 27 -- (d149-67-33-174.col.wideopenwest.com [67.149.174.33]) 9, 27 -- by er6s1.eng.ohio-state.edu (PMDF V6.2-1x11 #31056) 9, 27 -- with ESMTPA id {01MHOEES1H3GA9S4CM-at-er6s1.eng.ohio-state.edu} ; Mon, 9, 27 -- 11 Jun 2007 21:41:00 -0400 (EDT) 9, 27 -- Date: Mon, 11 Jun 2007 21:40:34 -0400 9, 27 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 9, 27 -- Subject: Re: [Microscopy] AskAMicroscopist: Beam voltage choice to minimise 9, 27 -- charging 9, 27 -- In-reply-to: {200706120026.l5C0QWb1032015-at-ns.microscopy.com} 9, 27 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 9, 27 -- To: L.Ryves-at-physics.usyd.edu.au 9, 27 -- Cc: microscopy-at-microscopy.com 9, 27 -- Message-id: {7.0.1.0.2.20070611212812.010d11f8-at-osu.edu} 9, 27 -- MIME-version: 1.0 9, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 9, 27 -- Content-type: text/plain; charset=us-ascii; format=flowed 9, 27 -- X-Env-From: auth/colijn.1-at-osu.edu 9, 27 -- References: {200706120026.l5C0QWb1032015-at-ns.microscopy.com} ==============================End of - Headers==============================
That is quite probably true. That is why I use 1KV and then 2KV. Beyond these values, specimens charge too much to be of use....IMO.
Other ILDs besides SiO2 are quite interesting and challenging. Of course, the gate oxides are another challenge.
gary g.
At 05:42 PM 6/11/2007, you wrote:
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First, you want to use a fast scan and average the frames. Just use enough frames in the average so that the updated image doesn't take too long. This is a dynamic affect and helps a lot. Slow scans are more difficult to set up the low voltage imaging. I can tell you that from personal experience on an older non-digital Hitachi S-900. When you went to slow scan to shoot the picture on Polaroid after using TV rates to set up a good charge balance image, you got charging in the recorded image.
Secondly, you need to find the correct voltage for charge balance. This is where the number of electrons (BSE and SE) is the same as incident number of electrons, i.e. beam current. For most insulators, as mentioned before, the voltage will be somewhere between 1 and 2 kV for a flat sample at zero tilt. If I remember correctly, go to a mag of about 1000 X, stay there for a little bit, then up the mag to 5000 X and stay there for about 20-30 seconds, then return quickly back to 1000 X. If you don't have charge balance, you will see a box in the lower mag image. Look quickly, because it will change. If the contrast of the box is bright relative to the low mag area, then your box had charged negatively and you are above the balance accelerating voltage. If the box was dark, then you were below the voltage and you have to increase the voltage. If it doesn't change contrast, you were JUST right. Eat your porridge and go take some nice pictures after your nap.
Also note, that for tilted samples the voltage value will increase. For example, on the JEOL Auger system, you could take uncoated insulators and do analysis at a beam voltage of 3 kV if the sample was tilted to an angle of about 70 degrees. This is because of the increase in both BSE and SE signals at the higher tilt angles. Essentially, the voltage shifted from about 1 kV at zero tilt to 3 kV at 70 degrees.
Hope this helps.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
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This is summarized in Goldstein's section 4.8.2., which is titled Charging.
Scanning Electron Microscopy and X-Ray Microanalysis, A Text for Biologists, Materials Scientists, and Geologists, 2nd Ed., Goldstein, J.I., et al., 1992.
-- Bernard R. Cuzzillo, Ph.D., P.E. President, Mechanical Engineer, and Fire Scientist Berkeley Research Company (BRC) 600 Addison Street Berkeley, CA 94710-1920 USA
bernard-at-berkeleyrc.com
==============================Original Headers============================== 4, 28 -- From biggerdadda-at-gmail.com Tue Jun 12 01:07:07 2007 4, 28 -- Received: from an-out-0708.google.com (an-out-0708.google.com [209.85.132.246]) 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5C676GN006249 4, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Jun 2007 01:07:07 -0500 4, 28 -- Received: by an-out-0708.google.com with SMTP id b33so412916ana 4, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 23:07:06 -0700 (PDT) 4, 28 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 4, 28 -- d=gmail.com; s=beta; 4, 28 -- h=domainkey-signature:received:received:message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 4, 28 -- b=WvRiehw+6OLNtmCLwELIiTp3iavvD0+LbWuvAOouhbpW7ONHYh+fVM7oQpyF5BxevGr1TFNL0bQS8tiTMPzRjHVTM/hNXBthFjxLgyLg3ol/VAyeIGpji81w/L6NGCsj4mJQaZNVSa/6v1L5sFQLhgf+gcidKjZpI016Ar6h18c= 4, 28 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 28 -- d=gmail.com; s=beta; 4, 28 -- h=received:message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 4, 28 -- b=hIjgZA7Fbr+Wzz+jUYROy95/KJAyPDyEOdhXaAJrQV7r9XUgMTY9b2+ddLr8uku8hd2+m1KOn6YjRdWFYYraZWo+qFxTCDqDCK5O0Zk3zOHb0VimdQYsIbPzKA9hUu3gpTliLZj/ehjIVuG1jaU9aCIEY5/bcuaukaW/wxY/POQ= 4, 28 -- Received: by 10.100.153.17 with SMTP id a17mr3799768ane.1181628426573; 4, 28 -- Mon, 11 Jun 2007 23:07:06 -0700 (PDT) 4, 28 -- Received: by 10.100.13.19 with HTTP; Mon, 11 Jun 2007 23:07:06 -0700 (PDT) 4, 28 -- Message-ID: {ef580e610706112307n35bde340na917f752fcc69d87-at-mail.gmail.com} 4, 28 -- Date: Mon, 11 Jun 2007 23:07:06 -0700 4, 28 -- From: "Bernard R. Cuzzillo, Ph.D., P.E." {bernard-at-berkeleyrc.com} 4, 28 -- Sender: biggerdadda-at-gmail.com 4, 28 -- To: Microscopy-at-microscopy.com 4, 28 -- Subject: Re: Specimen charging reference 4, 28 -- MIME-Version: 1.0 4, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 28 -- Content-Transfer-Encoding: 7bit 4, 28 -- Content-Disposition: inline 4, 28 -- X-Google-Sender-Auth: cb49c611c4954e3f ==============================End of - Headers==============================
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This is summarized in Goldstein's section 4.8.2., } which is titled Charging. } } Scanning Electron Microscopy and X-Ray } Microanalysis, A Text for } Biologists, Materials Scientists, and Geologists, } 2nd Ed., Goldstein, } J.I., et al., 1992. } } -- } Bernard R. Cuzzillo, Ph.D., P.E. } President, Mechanical Engineer, and Fire Scientist } Berkeley Research Company (BRC) } 600 Addison Street } Berkeley, CA 94710-1920 } USA } } bernard-at-berkeleyrc.com } } ==============================Original } Headers============================== } 4, 28 -- From biggerdadda-at-gmail.com Tue Jun 12 } 01:07:07 2007 } 4, 28 -- Received: from an-out-0708.google.com } (an-out-0708.google.com [209.85.132.246]) } 4, 28 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l5C676GN006249 } 4, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 12 } Jun 2007 01:07:07 -0500 } 4, 28 -- Received: by an-out-0708.google.com with
A number of people have already explained the basic ideas and given you relevant references. I just wish to add that you can find experimental curves of secondary yield vs. acceleration voltage (including curves for SiO2) at the webaddress
http://www.mc-set.com/bse/
Best regards,
Jørgen B. Bilde-Sørensen senior scientist, ph.d. Phone direct +45 4677 5802 j.bilde-at-risoe.dk
Materials Research Department Risø National Laboratory Technical University of Denmark - DTU Building 228, P.O. Box 49 DK-4000 Roskilde, Denmark Tel +45 4677 5700 Fax +45 4677 5758 www.risoe.dk
X-from 1 January 2007, Risø National Laboratory, the Danish Institute for Food and Veterinary Research, the Danish Institute for Fisheries Research, the Danish National Space Center and the Danish Transport Research Institute have been merged with the Technical University of Denmark (DTU) with DTU as the continuing unit.
-----Original Message----- X-from: L.Ryves-at-physics.usyd.edu.au [mailto:L.Ryves-at-physics.usyd.edu.au] Sent: Tuesday, June 12, 2007 2:30 AM To: j.bilde-at-risoe.dk
This Question was submitted to Ask-A-Microscopist by (L.Ryves-at-physics.usyd.edu.au) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, June 11, 2007 at 09:13:35 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both L.Ryves-at-physics.usyd.edu.au as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: L.Ryves-at-physics.usyd.edu.au Name: Luke Ryves
Organization: School of Physics / University of Sydney
Education: Graduate College
Location: Sydney, NSW, Australia
Title: Beam voltage choice to minimise charging
Question: Hi,
I'm just writing up my thesis and want to include some SEM images that were acquired for me ages ago. The samples were metal particles on SiO2 surfaces. At the time that we were taking the images I remember the microscopist explaining that the correct energy (2 to 5kV on a FEG SEM) can reduce charging, but I can't find a reference to this effect. Can you help me out by explaining how to choose the beam energy, and perhaps point me in the direction of some references?
Thanks to everybody who replied to my query about imaging carbon nanoparticles in tissue. I will prepare a summary of the replies soon and post it for the list.
As usual, this list has been a great resource. Thanks again.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 5, 23 -- From TindallR-at-missouri.edu Tue Jun 12 08:43:12 2007 5, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5CDhBU0017561 5, 23 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jun 2007 08:43:12 -0500 5, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 5, 23 -- Tue, 12 Jun 2007 08:43:11 -0500 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 23 -- Content-class: urn:content-classes:message 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="us-ascii" 5, 23 -- Subject: Carbon nanos in tissue 5, 23 -- Date: Tue, 12 Jun 2007 08:43:11 -0500 5, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BAFF-at-UM-XMAIL08.um.umsystem.edu} 5, 23 -- X-MS-Has-Attach: 5, 23 -- X-MS-TNEF-Correlator: 5, 23 -- Thread-Topic: Carbon nanos in tissue 5, 23 -- Thread-Index: Aces954zmMRlK74HSEm2B+CV51la1g== 5, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- X-OriginalArrivalTime: 12 Jun 2007 13:43:11.0342 (UTC) FILETIME=[9E2430E0:01C7ACF7] 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5CDhBU0017561 ==============================End of - Headers==============================
Stephanie: the 3rd edition is the most recent, but I think the 2nd edition is more useful to the novice/intermediate user. The section cited in the message by Dr. Cuzzillo is for the 2nd edition and is not in the 3rd edition as its own section. The information may be scattered around in Section 4 but I haven't sat down and looked for it.
Just an FYI: if you buy the 3rd edition as a used book, make sure it has the CD with it.
nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } After an internet search I found this book. Is this } the last edition of the same book? } } Scanning Electron Microscopy and X-ray Microanalysis } Goldstein, J., Newbury, D.E., Joy, D.C., Lyman, C.E., } Echlin, P., Lifshin, E., Sawyer, L.C., Michael, J.R. } } 3rd ed. 2003. Corr. 4th printing, 2007, 586 p., with 5 } pg 4/C insert, Hardcover } } Best regards, } } Stephane } } --- bernard-at-berkeleyrc.com wrote: } } ---------------------------------------------------------------------------- } } This is summarized in Goldstein's section 4.8.2., } } which is titled Charging. } } } } Scanning Electron Microscopy and X-Ray } } Microanalysis, A Text for } } Biologists, Materials Scientists, and Geologists, } } 2nd Ed., Goldstein, } } J.I., et al., 1992. } } } } -- } } Bernard R. Cuzzillo, Ph.D., P.E. } } President, Mechanical Engineer, and Fire Scientist } } Berkeley Research Company (BRC) } } 600 Addison Street } } Berkeley, CA 94710-1920 } } USA } } } } bernard-at-berkeleyrc.com } } } } ==============================Original } } Headers============================== } } 4, 28 -- From biggerdadda-at-gmail.com Tue Jun 12 } } 01:07:07 2007 } } 4, 28 -- Received: from an-out-0708.google.com } } (an-out-0708.google.com [209.85.132.246]) } } 4, 28 -- by ns.microscopy.com } } (8.12.11.20060308/8.12.8) with ESMTP id } } l5C676GN006249 } } 4, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 12 } } Jun 2007 01:07:07 -0500 } } 4, 28 -- Received: by an-out-0708.google.com with
Is there a website for attendees looking to share hotel rooms for the MandM meeting in Ft. Lauderdale?
thanks, Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
************************************************************************ ****** The box said "Requires WindowsXP or better", so I bought a Macintosh. ************************************************************************ ******
==============================Original Headers============================== 7, 24 -- From glenmac-at-u.washington.edu Wed Jun 13 12:50:47 2007 7, 24 -- Received: from mxout3.cac.washington.edu (mxout3.cac.washington.edu [140.142.32.166]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5DHolF1014877 7, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jun 2007 12:50:47 -0500 7, 24 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.33.9] (may be forged)) 7, 24 -- by mxout3.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.05) with ESMTP id l5DHoktD002647 7, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 7, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jun 2007 10:50:46 -0700 7, 24 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) 7, 24 -- (authenticated authid=glenmac) 7, 24 -- by smtp.washington.edu (8.13.7+UW06.06/8.13.7+UW07.03) with ESMTP id l5DHokkR006833 7, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 7, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jun 2007 10:50:46 -0700 7, 24 -- Mime-Version: 1.0 (Apple Message framework v752.2) 7, 24 -- Content-Transfer-Encoding: 7bit 7, 24 -- Message-Id: {987A552F-56B8-42F4-8EC5-5CEFBBC9729A-at-u.washington.edu} 7, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 24 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {Microscopy-at-microscopy.com} 7, 24 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 7, 24 -- Subject: MandM roommate site 7, 24 -- Date: Wed, 13 Jun 2007 10:50:44 -0700 7, 24 -- X-Mailer: Apple Mail (2.752.2) 7, 24 -- X-PMX-Version: 5.3.1.294258, Antispam-Engine: 2.5.1.298604, Antispam-Data: 2007.6.13.102633 7, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
Sorry Glen, but there is no site like this setup by the Meeting.
Nestor Your Friendly Neighborhood SysOp
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-- =========================================== Dr. Nestor J. Zaluzec Argonne National Lab Electron Microscopy Center Materials Science Division/Bldg 212 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov =========================================== TPMLab: http://tpm.amc.anl.gov MMSite: http://www.amc.anl.gov ===========================================
The box said ... "This program requires Win 95/98/NT or better..." So I bought a Mac !
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==============================Original Headers============================== 11, 16 -- From zaluzec-at-aaem.amc.anl.gov Wed Jun 13 13:04:39 2007 11, 16 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3]) 11, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5DI4d0a026455 11, 16 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jun 2007 13:04:39 -0500 11, 16 -- Received: from [146.139.72.105] (aem105.amc.anl.gov [146.139.72.105]) 11, 16 -- by aaem.amc.anl.gov (8.12.11.20060308/8.12.10) with ESMTP id l5DI4cja008110 11, 16 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jun 2007 13:04:39 -0500 11, 16 -- Mime-Version: 1.0 11, 16 -- Message-Id: {p0624080ec295e0ed3803-at-[146.139.72.105]} 11, 16 -- In-Reply-To: {200706131750.l5DHolXb014884-at-ns.microscopy.com} 11, 16 -- References: {200706131750.l5DHolXb014884-at-ns.microscopy.com} 11, 16 -- Date: Wed, 13 Jun 2007 13:04:36 -0500 11, 16 -- To: microscopy-at-microscopy.com 11, 16 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} 11, 16 -- Subject: Re: This is no MandM roommate site 11, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I have recently been having problems with my field emission scope lately at magnifications above 50KX. The image seems to swim and sparkles are seen and the focus boundaries. A slow scan image impossible to acquire. The ground is suspected. It is currently grounded to the building at the same location as all the rest of the analytical equipment. Our electricians are in the process of rerunning a separate ground for the scope. They are going to put an eight foot copper rod in the earth outside of my lab drill hole in the wall for wires to go through and attach it to the ground wires to the back of the scope. I was wondering how the rest of have run the grounds for your scopes. Any advice would be appreciated.
Lori Ables Solutia, Inc. laable-at-solutia.com
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==============================Original Headers============================== 6, 33 -- From laable-at-solutia.com Thu Jun 14 08:50:38 2007 6, 33 -- Received: from plmler9.mail.eds.com (plmler9.mail.eds.com [199.228.142.117]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EDoctm031340 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:38 -0500 6, 33 -- Received: from plmlir3.mail.eds.com (plmlir3-2.mail.eds.com [199.228.142.133]) 6, 33 -- by plmler9.mail.eds.com (8.13.8/8.13.8) with ESMTP id l5EDoYCV022081 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:36 -0500 6, 33 -- Received: from plmlir3.mail.eds.com (localhost.localdomain [127.0.0.1]) 6, 33 -- by plmlir3.mail.eds.com (8.13.8/8.12.10) with ESMTP id l5EDoCXS028914 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:12 -0500 6, 33 -- Received: from mailgw2.solutia.com ([205.191.166.96]) 6, 33 -- by plmlir3.mail.eds.com (8.13.8/8.12.10) with ESMTP id l5EDoBND028887 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:12 -0500 6, 33 -- Received: from USAHMSLSOIEX2.soi.dir.solutia.com ([205.191.166.99]) by mailgw2.solutia.com with Microsoft SMTPSVC(6.0.3790.1830); Thu, 14 Jun 2007 09:50:11 -0400 6, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.2826 6, 33 -- Content-Class: urn:content-classes:message 6, 33 -- MIME-Version: 1.0 6, 33 -- Content-Type: text/plain; 6, 33 -- charset="us-ascii" 6, 33 -- Subject: SEM Ground 6, 33 -- Date: Thu, 14 Jun 2007 09:50:08 -0400 6, 33 -- Message-ID: {7B97BC1F2986504EABA8A0B1C780A5C301E77C67-at-USAHMSLSOIEX2.soi.dir.solutia.com} 6, 33 -- Importance: normal 6, 33 -- Priority: normal 6, 33 -- X-MS-Has-Attach: 6, 33 -- X-MS-TNEF-Correlator: 6, 33 -- Thread-Topic: SEM Ground 6, 33 -- thread-index: AceuiupcKKVbl+U3T3uHsV9HIQBKdA== 6, 33 -- From: "Ables, Lori A" {laable-at-solutia.com} 6, 33 -- To: {Microscopy-at-Microscopy.Com} 6, 33 -- X-OriginalArrivalTime: 14 Jun 2007 13:50:11.0422 (UTC) FILETIME=[ED5ACFE0:01C7AE8A] 6, 33 -- Content-Transfer-Encoding: 8bit 6, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5EDoctm031340 ==============================End of - Headers==============================
You have not described the operating conditions or the samples. Has anything changed in those regards? Perhaps the matter is one of sample charging more so that instrumentation problems. I would certainly address those issues first with an application specialist or the list before resorting to the wiring change. That is not likely to be cheap.
If it is the ground, it sounds like the remedy is reasonable. It sounds like your people are on the right path.
Warren
________________________________________ X-from: laable-at-solutia.com [mailto:laable-at-solutia.com] Sent: Thu 6/14/2007 8:51 AM To: wesaia-at-iastate.edu
To All Listers,
I have recently been having problems with my field emission scope lately at magnifications above 50KX. The image seems to swim and sparkles are seen and the focus boundaries. A slow scan image impossible to acquire. The ground is suspected. It is currently grounded to the building at the same location as all the rest of the analytical equipment. Our electricians are in the process of rerunning a separate ground for the scope. They are going to put an eight foot copper rod in the earth outside of my lab drill hole in the wall for wires to go through and attach it to the ground wires to the back of the scope. I was wondering how the rest of have run the grounds for your scopes. Any advice would be appreciated.
Lori Ables Solutia, Inc. laable-at-solutia.com
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==============================Original Headers============================== 11, 31 -- From wesaia-at-iastate.edu Thu Jun 14 10:37:07 2007 11, 31 -- Received: from mailhub-3.iastate.edu (mailhub-3.iastate.edu [129.186.140.13]) 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EFb7CG013672 11, 31 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 14 Jun 2007 10:37:07 -0500 11, 31 -- Received: from mailout-2.iastate.edu (mailout-2.iastate.edu [129.186.140.2]) 11, 31 -- by mailhub-3.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id l5EFb6XL005530; 11, 31 -- Thu, 14 Jun 2007 10:37:06 -0500 11, 31 -- Received: from (owa.eng.iastate.edu [129.186.23.85]) by mailout-2.iastate.edu with smtp 11, 31 -- id 4744_9a3b25d0_1a8c_11dc_996b_001372578af6; 11, 31 -- Thu, 14 Jun 2007 10:33:29 -0500 11, 31 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 11, 31 -- Thu, 14 Jun 2007 10:37:07 -0500 11, 31 -- Content-class: urn:content-classes:message 11, 31 -- MIME-Version: 1.0 11, 31 -- Content-Type: text/plain; 11, 31 -- charset="iso-8859-1" 11, 31 -- Subject: RE: [Microscopy] SEM Ground 11, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 31 -- Date: Thu, 14 Jun 2007 10:37:41 -0500 11, 31 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72701A9EDFC-at-maire.eng.iastate.edu} 11, 31 -- X-MS-Has-Attach: 11, 31 -- X-MS-TNEF-Correlator: 11, 31 -- Thread-Topic: [Microscopy] SEM Ground 11, 31 -- Thread-Index: AceuiyhSKgw8zd0uSyisUNdBIUpgkAAAIZZ/ 11, 31 -- References: {200706141351.l5EDpmKh032623-at-ns.microscopy.com} 11, 31 -- From: "Straszheim, Warren E [CCE E]" {wesaia-at-iastate.edu} 11, 31 -- To: "MSA" {Microscopy-at-msa.microscopy.com} 11, 31 -- Cc: {laable-at-solutia.com} 11, 31 -- X-OriginalArrivalTime: 14 Jun 2007 15:37:07.0035 (UTC) FILETIME=[DD5BAAB0:01C7AE99] 11, 31 -- Content-Transfer-Encoding: 8bit 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5EFb7CG013672 ==============================End of - Headers==============================
Greetings all--I am seeking input on what appears to be Carbon contamination. Here is the situation.
Take a Pella 16111-9 stub out of bag and put on holder. Take another stub out of bag and sputter coat with Pd and put on holder. Do EDS on both.
un-coated: wt% at% C 7.5 15 O 4.5 7 Al 88 78
coated: C 23 38 O 6.5 8 Al 71 53
SEM is Zeiss Supra 55VP with Edwards XDS10 dry scroll pump and turbo. Coater is Denton Desk IV with Edwards XDS5 and turbo.
The goal of using non-oil pumps was to reduce hydrocarbon contamination. So, where is the C coming from? Nothing has been done to the scroll pumps since new. There are kits for repairing them but when is this necessary and what would indicate that it be done? Would high C be an indicator?
I'm stumped on this one.
Any ideas?
gary g.
==============================Original Headers============================== 10, 17 -- From gary-at-gaugler.com Thu Jun 14 11:54:47 2007 10, 17 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5EGslUC027192 10, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 11:54:47 -0500 10, 17 -- Message-Id: {200706141654.l5EGslUC027192-at-ns.microscopy.com} 10, 17 -- Received: (qmail 15711 invoked from network); 14 Jun 2007 09:54:47 -0700 10, 17 -- Received: by simscan 1.1.0 ppid: 15708, pid: 15709, t: 0.1452s 10, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 17 -- by qsmtp4 with SMTP; 14 Jun 2007 09:54:46 -0700 10, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 17 -- Date: Thu, 14 Jun 2007 09:54:46 -0800 10, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 10, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 17 -- Subject: Carbon "contamination" 10, 17 -- Mime-Version: 1.0 10, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-20B34597 ==============================End of - Headers==============================
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Can't help with the uncoated stub, but most of the "C Ka" you are seeing is presumably from the Pd Mz line which is at 43.36 A (vs the nominal 44.0 A for C Ka).
John -- ======================================================== John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480 Cameron Electron Microprobe Lab lab: (608) 265-4798 Dept of Geology & Geophysics fax: (608) 262-0693 University of Wisconsin home: (608) 274-2245 1215 West Dayton St. email: johnf-at-geology.wisc.edu Madison, WI 53706 amateur radio: WA3BTA Personal http://www.geology.wisc.edu/~johnf/ Probe lab http://www.geology.wisc.edu/~johnf/sx51.html Probe Sign Up Calender: http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi
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==============================Original Headers============================== 5, 25 -- From johnf-at-geology.wisc.edu Thu Jun 14 12:30:30 2007 5, 25 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EHUUQO007298 5, 25 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 12:30:30 -0500 5, 25 -- Received: from localhost (localhost [127.0.0.1]) 5, 25 -- by localhost (Postfix) with ESMTP id 3ED2020D06; 5, 25 -- Thu, 14 Jun 2007 12:30:30 -0500 (CDT) 5, 25 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) 5, 25 -- by localhost (geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 5, 25 -- with ESMTP id 13024-01; Thu, 14 Jun 2007 12:30:17 -0500 (CDT) 5, 25 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57]) 5, 25 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 5, 25 -- (No client certificate requested) 5, 25 -- by ice.geology.wisc.edu (Postfix) with ESMTP id 79EBB20D19; 5, 25 -- Thu, 14 Jun 2007 12:30:17 -0500 (CDT) 5, 25 -- Mime-Version: 1.0 5, 25 -- Message-Id: {p06230903c2972a7aaaed-at-[144.92.206.57]} 5, 25 -- In-Reply-To: {200706141659.l5EGxk54002427-at-ns.microscopy.com} 5, 25 -- References: {200706141659.l5EGxk54002427-at-ns.microscopy.com} 5, 25 -- Date: Thu, 14 Jun 2007 12:30:13 -0500 5, 25 -- To: gary-at-gaugler.com, microscopy-at-microscopy.com 5, 25 -- From: John Fournelle {johnf-at-geology.wisc.edu} 5, 25 -- Subject: Re: [Microscopy] Carbon "contamination" 5, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 25 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu ==============================End of - Headers==============================
One can buy a 40-100x oil objective with an iris diaphram. What is the theoretical difference in performance between imaging with a low NA dry objective (.6NA for example) and an equivalent magnification, high NA oil objective with the iris partially closed to reduce the NA to .6? Should they be similar? Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 5, 19 -- From david.knecht-at-uconn.edu Thu Jun 14 12:40:46 2007 5, 19 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EHekrM018923 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 12:40:46 -0500 5, 19 -- Received: from [137.99.46.174] (d46h174.public.uconn.edu [137.99.46.174]) 5, 19 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id l5EHeffX009795 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 13:40:41 -0400 5, 19 -- Mime-Version: 1.0 (Apple Message framework v752.3) 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- Message-Id: {D06FF75E-9614-4D26-BA77-9425A50AFCAD-at-uconn.edu} 5, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 19 -- To: microscopy {microscopy-at-microscopy.com} 5, 19 -- From: David Knecht {david.knecht-at-uconn.edu} 5, 19 -- Subject: objective NA 5, 19 -- Date: Thu, 14 Jun 2007 13:40:34 -0400 5, 19 -- X-Mailer: Apple Mail (2.752.3) 5, 19 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information. 5, 19 -- X-UConn-MailScanner: Found to be clean 5, 19 -- X-UConn-MailScanner-SpamCheck: ==============================End of - Headers==============================
The electricians have finished my ground and the result is amazing. I can now obtain a decent slow scan image at 500KX. Thanks to all who replied.
Lori
-----Original Message----- X-from: Ables, Lori A Sent: Thursday, June 14, 2007 8:50 AM To: ' (Microscopy-at-Microscopy.Com)'
Gary,
You're pretty clever, Gary, and I'm sure you've already done this. But......have you checked for any exposed wiring, gaskets or seals near the target that might be degraded when the plasma is activated? Are you using Argon gas to vent the system and as the source of plasma? If so, how clean is the gas used to generate plasma? Sometimes, gas cylinders contain traces of oil (as might the pressure regulators). Check the threads for the presence of a lubricant. Anyone else using the system? If so, they may have left some contamination inside the chamber (like finger oils).
If you are still having problems, try putting a coated TEM grid inside the chamber and examine it for traces of contamination. Sometimes, "seeing" the contamination is a clue to where it may be coming from.
Good luck.......
John B.
} Greetings all--I am seeking input on what appears } to be Carbon contamination. Here is the situation. } } Take a Pella 16111-9 stub out of bag and put on } holder. Take another stub out of bag and sputter } coat with Pd and put on holder. Do EDS on both. } The goal of using non-oil pumps was to reduce hydrocarbon } contamination. So, where is the C coming from? Nothing } has been done to the scroll pumps since new. There are } kits for repairing them but when is this necessary and } what would indicate that it be done? Would high C } be an indicator? }
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Lori, I am pleased that you saw such an improvement. Your original ground connection must have been pretty bad!
Because of the importance of an electrically quiet and isolated instrument ground, most instrument manufacturers won't install the instrument unless one is available. For example FEI quotes a less than 0.1 ohm resistance requirement and that doesn't even address the electrical noise issue.
In the new integrated science complex (underground shared instrumentation facilities) building that we are putting the finishing touches on here at Univ of Oregon, we designed in separate isolated, instrument grounds for each instrument. We had to negotiate with the electrical inspector to get this through and it wasn't easy. john
At 10:49 AM 6/14/2007, you wrote:
} All, } } The electricians have finished my ground and the result is } amazing. I can now obtain a decent slow scan image at 500KX. Thanks } to all who replied. } } Lori } } -----Original Message----- } X-from: Ables, Lori A } Sent: Thursday, June 14, 2007 8:50 AM } To: ' (Microscopy-at-Microscopy.Com)' } Subject: SEM Ground } } To All Listers, } } I have recently been having problems with my field emission scope } lately at magnifications above 50KX. The image seems to swim and } sparkles are seen and the focus boundaries. A slow scan image } impossible to acquire. The ground is suspected. It is currently } grounded to the building at the same location as all the rest of the } analytical equipment. Our electricians are in the process of } rerunning a separate ground for the scope. They are going to put an } eight foot copper rod in the earth outside of my lab drill hole in } the wall for wires to go through and attach it to the ground wires } to the back of the scope. I was wondering how the rest of have run } the grounds for your scopes. Any advice would be appreciated. } } Lori Ables } Solutia, Inc. } laable-at-solutia.com } } } This electronic mail message is intended exclusively for the } individual or entity to which it is addressed. } This message, together with any attachment, may contain Solutia } confidential and privileged information. } The recipient is hereby put on notice to treat the information as } confidential and privileged and to not disclose or use the } information except as authorized by Solutia. } Any unauthorized review, printing, retention, copying, disclosure, } distribution, retransmission, dissemination or other use of, or } taking of any action in reliance upon, this information by persons } or entities other than the intended recipient is prohibited. If you } received this message in error, please immediately contact the } sender by reply email and delete all copies of the material from any } computer. Thank you for your cooperation.
==============================Original Headers============================== 7, 21 -- From donovan-at-uoregon.edu Thu Jun 14 13:23:37 2007 7, 21 -- Received: from smtp.uoregon.edu (mserv4.uoregon.edu [128.223.142.54]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EINai4021959 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 13:23:37 -0500 7, 21 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.92.75]) 7, 21 -- (authenticated bits=0) 7, 21 -- by smtp.uoregon.edu (8.13.8/8.13.8) with ESMTP id l5EINaWW007377 7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 11:23:36 -0700 7, 21 -- Message-Id: {200706141823.l5EINaWW007377-at-smtp.uoregon.edu} 7, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 21 -- Date: Thu, 14 Jun 2007 11:22:31 -0700 7, 21 -- To: microscopy-at-microscopy.com 7, 21 -- From: John Donovan {donovan-at-uoregon.edu} 7, 21 -- Subject: Re: [Microscopy] FW: SEM Ground 7, 21 -- In-Reply-To: {200706141749.l5EHn4tW006932-at-ns.microscopy.com} 7, 21 -- References: {200706141749.l5EHn4tW006932-at-ns.microscopy.com} 7, 21 -- Mime-Version: 1.0 7, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 21 -- X-Virus-Scanned: ClamAV 0.90.3/3419/Thu Jun 14 06:49:39 2007 on mserv4 7, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Using basic theory the resolution should be the same [d = (Wavelength)/(2*NA)]. The fourier plane would be slightly different but not sure of the effects.
The depth of field should be (theoretically) better with an oil/water immersion stopped down. I haven't tested this personally.
Practically, I've noticed that there is less noise (scattered) light with both my oil and water immersion objectives.
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
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-----Original Message----- X-from: david.knecht-at-uconn.edu [mailto:david.knecht-at-uconn.edu] Sent: Thursday, June 14, 2007 1:45 PM To: ph2-at-sprynet.com
One can buy a 40-100x oil objective with an iris diaphram. What is the theoretical difference in performance between imaging with a low NA dry objective (.6NA for example) and an equivalent magnification, high NA oil objective with the iris partially closed to reduce the NA to .6? Should they be similar? Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 5, 19 -- From david.knecht-at-uconn.edu Thu Jun 14 12:40:46 2007 5, 19 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EHekrM018923 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 12:40:46 -0500 5, 19 -- Received: from [137.99.46.174] (d46h174.public.uconn.edu [137.99.46.174]) 5, 19 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id l5EHeffX009795 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 13:40:41 -0400 5, 19 -- Mime-Version: 1.0 (Apple Message framework v752.3) 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- Message-Id: {D06FF75E-9614-4D26-BA77-9425A50AFCAD-at-uconn.edu} 5, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 19 -- To: microscopy {microscopy-at-microscopy.com} 5, 19 -- From: David Knecht {david.knecht-at-uconn.edu} 5, 19 -- Subject: objective NA 5, 19 -- Date: Thu, 14 Jun 2007 13:40:34 -0400 5, 19 -- X-Mailer: Apple Mail (2.752.3) 5, 19 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information. 5, 19 -- X-UConn-MailScanner: Found to be clean 5, 19 -- X-UConn-MailScanner-SpamCheck: ==============================End of - Headers==============================
==============================Original Headers============================== 17, 27 -- From ph2-at-sprynet.com Thu Jun 14 13:47:28 2007 17, 27 -- Received: from elasmtp-galgo.atl.sa.earthlink.net (elasmtp-galgo.atl.sa.earthlink.net [209.86.89.61]) 17, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EIlRMm001829 17, 27 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 13:47:27 -0500 17, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 17, 27 -- s=dk20050327; d=sprynet.com; 17, 27 -- b=k5Yb3tsTpncKy3r432AnaZ79IlLFh7a51WJczzhv458UQrfX/VW6pVWngqFlZjHe; 17, 27 -- h=Received:From:To:Cc:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:X-MimeOLE:In-Reply-To:Message-ID:X-ELNK-Trace:X-Originating-IP; 17, 27 -- Received: from [75.61.18.94] (helo=user915fa8f284) 17, 27 -- by elasmtp-galgo.atl.sa.earthlink.net with asmtp (Exim 4.34) 17, 27 -- id 1HyuLv-0003Av-6M; Thu, 14 Jun 2007 14:47:27 -0400 17, 27 -- From: "Tony Havics" {ph2-at-sprynet.com} 17, 27 -- To: {david.knecht-at-uconn.edu} 17, 27 -- Cc: "Micrscopy Listserve" {microscopy-at-microscopy.com} 17, 27 -- Subject: RE: [Microscopy] objective NA 17, 27 -- Date: Thu, 14 Jun 2007 14:47:25 -0400 17, 27 -- MIME-Version: 1.0 17, 27 -- Content-Type: text/plain; 17, 27 -- charset="us-ascii" 17, 27 -- Content-Transfer-Encoding: 7bit 17, 27 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 17, 27 -- Thread-Index: Aceuq8mzPo2O3LLmSLSfM+5NwCzYQAAB8scA 17, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 17, 27 -- In-Reply-To: {200706141745.l5EHjOaj029640-at-ns.microscopy.com} 17, 27 -- Message-ID: {E1HyuLv-0003Av-6M-at-elasmtp-galgo.atl.sa.earthlink.net} 17, 27 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f9c4844ad2770a37891aa2f28f0fc702dd350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 17, 27 -- X-Originating-IP: 75.61.18.94 ==============================End of - Headers==============================
Thanks for the reply and to the others who have replied.
More data.
Gas is industrial welding Ar run through a Matheson molecular sieve (to dry and filter). SEM chamber uses industrial N2 also run through a molecular sieve.
Specimens are put in SEM chamber via Fjeld M-100 specimen load lock. This unit is pumped with small oil pump and turbo. Main SEM door is rarely opened. I have to check load lock pumps to see if it really is an oil roughing pump. I thought I got a dry unit for this too.
Pd target is from Refining Systems Las Vegas and is 99.5% pure. Trace elements do not include C. Coater is only used by myself. Chamber of coater is stainless steel (so it seems--but metal nevertheless). Only visible seal is the L ring rubber, or whatever, at the top.
This C issue just came up while trying to quant TaN on Si. It showed C that should not be there. So now I wonder about all spectra work and quants that include C. I can't think of a way to narrow down where the C is coming from and how to negate it. I will try cleaning the stubs and also try other stub types.
Exactly what are you saying about the TEM grid? The procedure is not clear to me. Are you saying I should coat it with Pd? Then what? I have STEM but not TEM.
gary g.
At 10:13 AM 6/14/2007, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 14, 20 -- From gary-at-gaugler.com Thu Jun 14 13:52:46 2007 14, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5EIqjCh013403 14, 20 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 13:52:46 -0500 14, 20 -- Message-Id: {200706141852.l5EIqjCh013403-at-ns.microscopy.com} 14, 20 -- Received: (qmail 12754 invoked from network); 14 Jun 2007 11:52:45 -0700 14, 20 -- Received: by simscan 1.1.0 ppid: 12751, pid: 12752, t: 0.2389s 14, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 14, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 14, 20 -- by qsmtp1 with SMTP; 14 Jun 2007 11:52:45 -0700 14, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 14, 20 -- Date: Thu, 14 Jun 2007 11:51:22 -0800 14, 20 -- To: bozzola-at-siu.edu 14, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 14, 20 -- Subject: Re: [Microscopy] Re: Carbon "contamination" 14, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 14, 20 -- In-Reply-To: {200706141813.l5EIDR6m013099-at-ns.microscopy.com} 14, 20 -- References: {200706141813.l5EIDR6m013099-at-ns.microscopy.com} 14, 20 -- Mime-Version: 1.0 14, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-27D32FCC ==============================End of - Headers==============================
Dear All, I did some EDS spectra on small steel particles. Specimen is uncoated on a adhesive C-tab. Energy had been 15 KV. I used a Roentec SLEW window detector. See images and spectra at www.elektronenmikroskopie.info/eds
Is there any other explaination for the titan peak to show up rather than it really is there in the specimen? Is there any possibility for fluorescense? Sorry in advance if this question is too simple for the list. I am not very experienced in interpreting eds spectra...
My first impression is that the titanium is real. It would be a little surprising for a regular stainless steel, but I see you also have cobalt peaks present. The peak around 7 kV is too intense to be only Fe K-beta. Co K-alpha is at 6.93 kV. You have a somewhat unusual specimen and Ti might be in order.
I don't know your x-ray system. I would highly recommend deconvoluting with the elements you know to be present and then examining the residuals from the fit. (Hopefully your system can show you the residuals. They are very helpful.) See what peaks or portions thereof are unaccounted for. I have had several different flavors of EDS systems over the years. They generally do a fair job of deconvolution _if_ you give them the right elements to begin with. For example, if I have a small mess around 2.3 kV and I let the EDS work with both S-K and Mo-L and Pb-M, it will generally give me a fair idea of which element is present if I have counted enough to well define my peaks.
The corollary is if you give the wrong elements or let the EDS pick the wrong elements on its own, you can get some quite screwy answers. Mo can turn into S. Ba can turn into Ti and Ag can turn into Ar.
Warren
-----Original Message----- X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de] Sent: Thursday, June 14, 2007 2:16 PM To: wesaia-at-iastate.edu
Dear All, I did some EDS spectra on small steel particles. Specimen is uncoated on a adhesive C-tab. Energy had been 15 KV. I used a Roentec SLEW window detector. See images and spectra at www.elektronenmikroskopie.info/eds
Is there any other explaination for the titan peak to show up rather than it really is there in the specimen? Is there any possibility for fluorescense? Sorry in advance if this question is too simple for the list. I am not very experienced in interpreting eds spectra...
HPD from EDAX works okay at low energy, but the labelling is not as sharp.
regards,
Jim
PS: OoO away.......
} From mail-at-ns.microscopy.com Thu Jun 14 12:52:28 2007 } Date: Thu, 14 Jun 2007 11:55:33 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: gary-at-gaugler.com } Reply-to: gary-at-gaugler.com } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] Carbon "contamination" } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Greetings all--I am seeking input on what appears } to be Carbon contamination. Here is the situation. } } Take a Pella 16111-9 stub out of bag and put on } holder. Take another stub out of bag and sputter } coat with Pd and put on holder. Do EDS on both. } } un-coated: } wt% at% } C 7.5 15 } O 4.5 7 } Al 88 78 } } coated: } C 23 38 } O 6.5 8 } Al 71 53 } } SEM is Zeiss Supra 55VP with Edwards XDS10 dry } scroll pump and turbo. Coater is Denton Desk IV } with Edwards XDS5 and turbo. } } The goal of using non-oil pumps was to reduce hydrocarbon } contamination. So, where is the C coming from? Nothing } has been done to the scroll pumps since new. There are } kits for repairing them but when is this necessary and } what would indicate that it be done? Would high C } be an indicator? } } I'm stumped on this one. } } Any ideas? } } gary g. } } } ==============================Original Headers============================== } 10, 17 -- From gary-at-gaugler.com Thu Jun 14 11:54:47 2007 } 10, 17 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) } 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5EGslUC027192 } 10, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 11:54:47 -0500 } 10, 17 -- Message-Id: {200706141654.l5EGslUC027192-at-ns.microscopy.com} } 10, 17 -- Received: (qmail 15711 invoked from network); 14 Jun 2007 09:54:47 -0700 } 10, 17 -- Received: by simscan 1.1.0 ppid: 15708, pid: 15709, t: 0.1452s } 10, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 } 10, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) } 10, 17 -- by qsmtp4 with SMTP; 14 Jun 2007 09:54:46 -0700 } 10, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 10, 17 -- Date: Thu, 14 Jun 2007 09:54:46 -0800 } 10, 17 -- To: MSA listserver {microscopy-at-microscopy.com} } 10, 17 -- From: Gary Gaugler {gary-at-gaugler.com} } 10, 17 -- Subject: Carbon "contamination" } 10, 17 -- Mime-Version: 1.0 } 10, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-20B34597 } ==============================End of - Headers============================== }
==============================Original Headers============================== 13, 12 -- From jquinn-at-www.matscieng.sunysb.edu Thu Jun 14 14:57:15 2007 13, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 13, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EJvELi017035 13, 12 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 14:57:14 -0500 13, 12 -- Received: (from jquinn-at-localhost) 13, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id l5EJr1B21280; 13, 12 -- Thu, 14 Jun 2007 15:53:01 -0400 13, 12 -- Date: Thu, 14 Jun 2007 15:53:01 -0400 13, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 13, 12 -- Message-Id: {200706141953.l5EJr1B21280-at-www.matscieng.sunysb.edu} 13, 12 -- To: gary-at-gaugler.com, microscopy-at-microscopy.com 13, 12 -- Subject: re: Pd two main M lines ==============================End of - Headers==============================
Do you observe the same effect with all detectors, (ie. A back scattered electron detector)? You might be having a problem with your secondary electron detector.
On 6/14/07 9:51 AM, "laable-at-solutia.com" {laable-at-solutia.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } To All Listers, } } I have recently been having problems with my field emission scope lately at } magnifications above 50KX. The image seems to swim and sparkles are seen and } the focus boundaries. A slow scan image impossible to acquire. The ground is } suspected. It is currently grounded to the building at the same location as } all the rest of the analytical equipment. Our electricians are in the process } of rerunning a separate ground for the scope. They are going to put an eight } foot copper rod in the earth outside of my lab drill hole in the wall for } wires to go through and attach it to the ground wires to the back of the } scope. I was wondering how the rest of have run the grounds for your scopes. } Any advice would be appreciated. } } Lori Ables } Solutia, Inc. } laable-at-solutia.com } } } This electronic mail message is intended exclusively for the individual or } entity to which it is addressed. } This message, together with any attachment, may contain Solutia confidential } and privileged information. } The recipient is hereby put on notice to treat the information as confidential } and privileged and to not disclose or use the information except as authorized } by Solutia. } Any unauthorized review, printing, retention, copying, disclosure, } distribution, retransmission, dissemination or other use of, or taking of any } action in reliance upon, this information by persons or entities other than } the intended recipient is prohibited. If you received this message in error, } please immediately contact the sender by reply email and delete all copies of } the material from any computer. Thank you for your cooperation. } } } ==============================Original Headers============================== } 6, 33 -- From laable-at-solutia.com Thu Jun 14 08:50:38 2007 } 6, 33 -- Received: from plmler9.mail.eds.com (plmler9.mail.eds.com } [199.228.142.117]) } 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l5EDoctm031340 } 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:38 -0500 } 6, 33 -- Received: from plmlir3.mail.eds.com (plmlir3-2.mail.eds.com } [199.228.142.133]) } 6, 33 -- by plmler9.mail.eds.com (8.13.8/8.13.8) with ESMTP id l5EDoYCV022081 } 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:36 -0500 } 6, 33 -- Received: from plmlir3.mail.eds.com (localhost.localdomain } [127.0.0.1]) } 6, 33 -- by plmlir3.mail.eds.com (8.13.8/8.12.10) with ESMTP id } l5EDoCXS028914 } 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:12 -0500 } 6, 33 -- Received: from mailgw2.solutia.com ([205.191.166.96]) } 6, 33 -- by plmlir3.mail.eds.com (8.13.8/8.12.10) with ESMTP id } l5EDoBND028887 } 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:12 -0500 } 6, 33 -- Received: from USAHMSLSOIEX2.soi.dir.solutia.com ([205.191.166.99]) } by mailgw2.solutia.com with Microsoft SMTPSVC(6.0.3790.1830); Thu, 14 Jun 2007 } 09:50:11 -0400 } 6, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.2826 } 6, 33 -- Content-Class: urn:content-classes:message } 6, 33 -- MIME-Version: 1.0 } 6, 33 -- Content-Type: text/plain; } 6, 33 -- charset="us-ascii" } 6, 33 -- Subject: SEM Ground } 6, 33 -- Date: Thu, 14 Jun 2007 09:50:08 -0400 } 6, 33 -- Message-ID: } {7B97BC1F2986504EABA8A0B1C780A5C301E77C67-at-USAHMSLSOIEX2.soi.dir.solutia.com} } 6, 33 -- Importance: normal } 6, 33 -- Priority: normal } 6, 33 -- X-MS-Has-Attach: } 6, 33 -- X-MS-TNEF-Correlator: } 6, 33 -- Thread-Topic: SEM Ground } 6, 33 -- thread-index: AceuiupcKKVbl+U3T3uHsV9HIQBKdA== } 6, 33 -- From: "Ables, Lori A" {laable-at-solutia.com} } 6, 33 -- To: {Microscopy-at-Microscopy.Com} } 6, 33 -- X-OriginalArrivalTime: 14 Jun 2007 13:50:11.0422 (UTC) } FILETIME=[ED5ACFE0:01C7AE8A] } 6, 33 -- Content-Transfer-Encoding: 8bit } 6, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id l5EDoctm031340 } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 25 -- From david.r.hull-at-nasa.gov Thu Jun 14 15:21:18 2007 6, 25 -- Received: from ndjsbar02.ndc.nasa.gov (ndjsbar02.ndc.nasa.gov [198.120.25.39]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EKLHiD028905 6, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 15:21:18 -0500 6, 25 -- Received: from ndjsxgw01.ndc.nasa.gov (ndjsxgw01.ndc.nasa.gov [129.166.32.111]) 6, 25 -- by ndjsbar02.ndc.nasa.gov (Spam Firewall) with ESMTP 6, 25 -- id EF4BD283987; Thu, 14 Jun 2007 15:21:16 -0500 (CDT) 6, 25 -- Received: from NDJSEVS23B.ndc.nasa.gov ([129.166.32.224]) by ndjsxgw01.ndc.nasa.gov with Microsoft SMTPSVC(6.0.3790.1830); 6, 25 -- Thu, 14 Jun 2007 15:21:16 -0500 6, 25 -- Received: from 129.166.32.12 ([129.166.32.12]) by NDJSEVS23B.ndc.nasa.gov ([129.166.32.227]) via Exchange Front-End Server mail01.ndc.nasa.gov ([129.166.32.102]) with Microsoft Exchange Server HTTP-DAV ; 6, 25 -- Thu, 14 Jun 2007 20:21:16 +0000 6, 25 -- User-Agent: Microsoft-Entourage/11.3.3.061214 6, 25 -- Date: Thu, 14 Jun 2007 16:21:15 -0400 6, 25 -- Subject: Re: [Microscopy] SEM Ground 6, 25 -- From: "Hull, David R" {David.R.Hull-at-nasa.gov} 6, 25 -- To: {laable-at-solutia.com} , {Microscopy-at-microscopy.com} 6, 25 -- Message-ID: {C2971B7B.670E%David.R.Hull-at-nasa.gov} 6, 25 -- Thread-Topic: [Microscopy] SEM Ground 6, 25 -- Thread-Index: AceuwY68zWJTXhq0EdyiPgAKldSx3A== 6, 25 -- In-Reply-To: {200706141351.l5EDpen4032477-at-ns.microscopy.com} 6, 25 -- Mime-version: 1.0 6, 25 -- Content-type: text/plain; 6, 25 -- charset="US-ASCII" 6, 25 -- Content-transfer-encoding: 7bit 6, 25 -- X-OriginalArrivalTime: 14 Jun 2007 20:21:16.0722 (UTC) FILETIME=[8FC37120:01C7AEC1] ==============================End of - Headers==============================
Electron/Ion Beam Instrument Engineer The University of Oregon's Center for Advanced Materials Characterization in Oregon (CAMCOR) is seeking applications for a full time staff position to begin September 2007. A strong background in maintaining, trouble shooting and upgrading electron/ion beam instruments and associated high voltage, vacuum, mechanical and electrical systems is required. Experience with x-ray diffraction instrumentation is also desirable. Salary range $60K-90K commensurate with experience.
This position will be located in the new Lorry Lokey Integrated Science Laboratory, a state of the art nano and micro science analytical instrument facility designed specifically for exceptional nano-science performance. It will house the latest electron, ion and x-ray beam instrumentation available including a Zeiss Ultra TFEM, FEI Quanta 200 E-SEM, Cameca SX50 and SX100 microprobes, Ion-TOF SIMS, Philips Auger Spectrometer, Philips TEM, Kratos XPS and various assorted coaters, etchers, and other vacuum deposition systems.
The successful candidate with have a BS in a beam microscopy related field and an extensive background in instrument field service with significant practical experience troubleshooting high vacuum electron and ion beam instrumentation at both the system and PC board levels. Must be able to read and understand schematics for electronic circuits and systems. The successful applicant will be involved in modifying/improving instrumentation capabilities to enable the equipment to more fully support unique research needs and will be expected to work intimately with the scientific staff and research faculty. We seek candidates with a demonstrated commitment to working effectively with students, faculty and staff from diverse backgrounds.
Interested persons should send a resume with a detailed description of work experience and skills, and arrange for two letters of recommendation to be sent to: CAMCOR Instrument Engineer Search Committee, 1253 University of Oregon, Eugene, OR, 97403-1253. To be assured of full consideration, application materials must be received by July 31, 2007, but the search will remain open until the position is filled. For further information, contact John Donovan (donovan-at-uoregon.edu).
University of Oregon is an AA/EEO employer committed to cultural diversity.
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Materials Engineer job opening -- Bodycote HIP (Hot Isostatic Pressing) in Andover, Massachusetts, has an immediate opening for an entry-level B.S. or M.S. materials or metallurgical engineer for our Technical Services group. The ideal candidate will have a strong interest and/or experience in metallography, microscopy and failure analysis. He/she will interact with both internal and external customers, so good communication skills are a must. A solid background in thermodynamics and physical metallurgy is also desired. Powder metallurgy, heat-treating and/or foundry knowledge is also a plus.
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==============================Original Headers============================== 8, 31 -- From Jane.LaGoy-at-Bodycote.com Thu Jun 14 15:36:47 2007 8, 31 -- Received: from mail161.messagelabs.com (mail161.messagelabs.com [216.82.253.115]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5EKakOL019724 8, 31 -- for {Microscopy-at-MSA.Microscopy.com} ; Thu, 14 Jun 2007 15:36:46 -0500 8, 31 -- X-VirusChecked: Checked 8, 31 -- X-Env-Sender: Jane.LaGoy-at-Bodycote.com 8, 31 -- X-Msg-Ref: server-2.tower-161.messagelabs.com!1181853404!3236203!1 8, 31 -- X-StarScan-Version: 5.5.12.11; banners=-,-,- 8, 31 -- X-Originating-IP: [209.235.7.177] 8, 31 -- Received: (qmail 22703 invoked from network); 14 Jun 2007 20:36:45 -0000 8, 31 -- Received: from 177-209.235.7.appsitehosting.com (HELO VM200EXC02.ad.bodycote.na) (209.235.7.177) 8, 31 -- by server-2.tower-161.messagelabs.com with SMTP; 14 Jun 2007 20:36:45 -0000 8, 31 -- Received: from VM200EXC01.ad.bodycote.na ([10.200.20.9]) by VM200EXC02.ad.bodycote.na with Microsoft SMTPSVC(6.0.3790.3959); 8, 31 -- Thu, 14 Jun 2007 16:36:44 -0400 8, 31 -- Content-Class: urn:content-classes:message 8, 31 -- MIME-Version: 1.0 8, 31 -- Content-Type: text/plain; 8, 31 -- charset="us-ascii" 8, 31 -- Subject: Materials Engineer position opening 8, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.3959 8, 31 -- Date: Thu, 14 Jun 2007 16:36:45 -0400 8, 31 -- Message-ID: {412949C8D462414B9BE7A0921A9C841608FEAA-at-VM200EXC01.ad.bodycote.na} 8, 31 -- X-MS-Has-Attach: 8, 31 -- X-MS-TNEF-Correlator: 8, 31 -- Thread-Topic: Materials Engineer position opening 8, 31 -- thread-index: Aceuw7lRzjm4nwJXQiG7pcIovLPqkg== 8, 31 -- From: "Jane LaGoy" {Jane.LaGoy-at-Bodycote.com} 8, 31 -- To: {Microscopy-at-MSA.Microscopy.com} 8, 31 -- X-OriginalArrivalTime: 14 Jun 2007 20:36:44.0309 (UTC) FILETIME=[B8A5FC50:01C7AEC3] 8, 31 -- Content-Transfer-Encoding: 8bit 8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5EKakOL019724 ==============================End of - Headers==============================
The only possible overlap with C and Pd is at Pd Mz=0.286KeV. But at 5KV beam, I would expect this Pd peak to be quite low.
Then, it still does not explain why I see C on an un-coated stub. The suggestion to clean some is good and I will do that. I checked the specimen holder and it is loaded with organics and C--likely from machining.
I can also try X-Checker and do Cu at 5KV and then try a Si die. This would eliminate any low KeV peaks near C. If I still see C, then...??? The low KV is necessary to analyze TaN since the N is oddly bound to the Ta. Different (higher) KV gives different results. I've done routing EDS on TiN and it is quite different.
A possible coating factor is the Ar cylinder. It is steel and is about seven years old. I wonder if over time, it oozes C into the Ar? A 2,000psi tank seems to last forever.
gary g.
At 11:58 AM 6/14/2007, you wrote:
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==============================Original Headers============================== 14, 20 -- From gary-at-gaugler.com Thu Jun 14 15:53:30 2007 14, 20 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5EKrUTC031482 14, 20 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 15:53:30 -0500 14, 20 -- Message-Id: {200706142053.l5EKrUTC031482-at-ns.microscopy.com} 14, 20 -- Received: (qmail 10432 invoked from network); 14 Jun 2007 13:53:29 -0700 14, 20 -- Received: by simscan 1.1.0 ppid: 10426, pid: 10427, t: 0.1195s 14, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 14, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 14, 20 -- by qsmtp2 with SMTP; 14 Jun 2007 13:53:29 -0700 14, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 14, 20 -- Date: Thu, 14 Jun 2007 13:53:28 -0800 14, 20 -- To: jquinn-at-www.matscieng.sunysb.edu 14, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 14, 20 -- Subject: Re: [Microscopy] re: Pd two main M lines 14, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 14, 20 -- In-Reply-To: {200706141958.l5EJwkaB019860-at-ns.microscopy.com} 14, 20 -- References: {200706141958.l5EJwkaB019860-at-ns.microscopy.com} 14, 20 -- Mime-Version: 1.0 14, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-46F525F ==============================End of - Headers==============================
The thin Pd coating may enhance the M peaks over the L peaks
Run a simulation with WinXRay at 5kV. See fourth image at this URL: http://www.matscieng.sunysb.edu/temp/gg/
You will see two Pd-M peaks are nearly the size of the Pd-Lb peak. You can tweak the thickness to change the M/L ratio.
Also, with the weak signal, you may be seeing secondary emission from your polymer window.
Also, carbon is everywhere. Fact of life. HF etch a silicon a chip from a silicon wafer. Take a spectrum. That will be your low limit on cleanliness of carbon (not oxygen). You could also Shirake etch the Si chip.
JQ
} From gary-at-gaugler.com Thu Jun 14 16:50:19 2007 } X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } Date: Thu, 14 Jun 2007 13:53:28 -0800 } To: jquinn-at-www.matscieng.sunysb.edu } From: Gary Gaugler {gary-at-gaugler.com} } Subject: Re: [Microscopy] re: Pd two main M lines } Cc: MSA listserver {microscopy-at-microscopy.com} } In-Reply-To: {200706141958.l5EJwkaB019860-at-ns.microscopy.com} } References: {200706141958.l5EJwkaB019860-at-ns.microscopy.com} } Mime-Version: 1.0 } Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-46F525F } } Check out the spectra at } } http://www.gaugler.com/coatedstub.pdf } } The only possible overlap with C and Pd is } at Pd Mz=0.286KeV. But at 5KV beam, I would } expect this Pd peak to be quite low. } } Then, it still does not explain why I see } C on an un-coated stub. The suggestion to } clean some is good and I will do that. I } checked the specimen holder and it is loaded } with organics and C--likely from machining. } } I can also try X-Checker and do Cu at 5KV } and then try a Si die. This would eliminate } any low KeV peaks near C. If I still see C, } then...??? The low KV is necessary to analyze } TaN since the N is oddly bound to the Ta. Different } (higher) KV gives different results. I've done } routing EDS on TiN and it is quite different. } } A possible coating factor is the Ar cylinder. It } is steel and is about seven years old. I wonder if } over time, it oozes C into the Ar? A 2,000psi } tank seems to last forever. } } gary g. } } } At 11:58 AM 6/14/2007, you wrote: } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Gary and company } } } } http://www.matscieng.sunysb.edu/temp/gg/ } } } } The link will vanish is a few weeks. } } } } The three spectra are calculations. } } } } The first is for C-K and O-K with 100cts. } } } } The second is for Pd-M with 100cts. } } } } The third is them combined. } } } } You would see the same with a real sample. } } } } HPD from EDAX works okay at low energy, but } } the labelling is not as sharp. } } } } regards, } } } } Jim } } } } PS: OoO away....... } } } } } From mail-at-ns.microscopy.com Thu Jun 14 12:52:28 2007 } } } Date: Thu, 14 Jun 2007 11:55:33 -0500 } } } To: jquinn-at-www.matscieng.sunysb.edu } } } From: gary-at-gaugler.com } } } Reply-to: gary-at-gaugler.com } } } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } } } Subject: [Microscopy] Carbon "contamination" } } } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } } } X-lewp: MicroscopyListSpam NAGS } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } ---------------------------------------------------------------------------- } } } } } } Greetings all--I am seeking input on what appears } } } to be Carbon contamination. Here is the situation. } } } } } } Take a Pella 16111-9 stub out of bag and put on } } } holder. Take another stub out of bag and sputter } } } coat with Pd and put on holder. Do EDS on both. } } } } } } un-coated: } } } wt% at% } } } C 7.5 15 } } } O 4.5 7 } } } Al 88 78 } } } } } } coated: } } } C 23 38 } } } O 6.5 8 } } } Al 71 53 } } } } } } SEM is Zeiss Supra 55VP with Edwards XDS10 dry } } } scroll pump and turbo. Coater is Denton Desk IV } } } with Edwards XDS5 and turbo. } } } } } } The goal of using non-oil pumps was to reduce hydrocarbon } } } contamination. So, where is the C coming from? Nothing } } } has been done to the scroll pumps since new. There are } } } kits for repairing them but when is this necessary and } } } what would indicate that it be done? Would high C } } } be an indicator? } } } } } } I'm stumped on this one. } } } } } } Any ideas? } } } } } } gary g. } } }
==============================Original Headers============================== 11, 12 -- From jquinn-at-www.matscieng.sunysb.edu Thu Jun 14 16:21:21 2007 11, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5ELLL6Q011139 11, 12 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 16:21:21 -0500 11, 12 -- Received: (from jquinn-at-localhost) 11, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id l5ELI3921642; 11, 12 -- Thu, 14 Jun 2007 17:18:03 -0400 11, 12 -- Date: Thu, 14 Jun 2007 17:18:03 -0400 11, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 11, 12 -- Message-Id: {200706142118.l5ELI3921642-at-www.matscieng.sunysb.edu} 11, 12 -- To: gary-at-gaugler.com, microscopy-at-microscopy.com 11, 12 -- Subject: Re: [Microscopy] re: Pd two main M lines ==============================End of - Headers==============================
Our lab has a Buehler IsoMet slow saw (model #11-1180, apparently 1980s vintage) that has ceased functioning. I've tracked the problem down to broken gear teeth on the shaft perpendicular to the motor's drive shaft. Does anyone else have a dead or unused IsoMet that they wouldn't mind someone cannibalizing? We can pay shipping and/or modestly for the equipment/parts. We also don't have the instructions or specifications anymore. If someone else does and there are good part descriptions, I'd be interested in a fax or scan of that page to help track down a replacement part. Thanks in advance!
Cheers, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu Personal Website: http://umn.edu/~frah0010
Please note: Sometimes the University's spam filters are over- protective and reject wanted messages, especially from free or overseas accounts. If your message is rejected or you are concerned that you did not receive a reply from me, please feel free to try my alternate email account: elleryfrahm-at-mac.com
==============================Original Headers============================== 6, 17 -- From frah0010-at-umn.edu Thu Jun 14 18:58:38 2007 6, 17 -- Received: from mta-w2.tc.umn.edu (mta-w2.tc.umn.edu [134.84.119.6]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5ENwcCp025383 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 18:58:38 -0500 6, 17 -- Received: from [10.0.1.3] (c-75-72-182-252.hsd1.mn.comcast.net [75.72.182.252]) 6, 17 -- by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 18:58:38 -0500 (CDT) 6, 17 -- X-Umn-Remote-Mta: [N] c-75-72-182-252.hsd1.mn.comcast.net [75.72.182.252] #+TS+AU+HN 6, 17 -- Mime-Version: 1.0 (Apple Message framework v752.2) 6, 17 -- Content-Transfer-Encoding: 7bit 6, 17 -- Message-Id: {1862217B-D7B4-46CA-99B6-0819AFC25E13-at-umn.edu} 6, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 6, 17 -- To: microscopy-at-microscopy.com 6, 17 -- From: Ellery Frahm {frah0010-at-umn.edu} 6, 17 -- Subject: Buehler IsoMet 6, 17 -- Date: Thu, 14 Jun 2007 18:58:37 -0500 6, 17 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nmedvitz-at-nephrocor.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: nmedvitz-at-nephrocor.com Name: Neil Medvitz
Organization: Bostwick Laboratories
Title-Subject: [Filtered] Advantages of having two TEMs in the same location rather than having one EM in two locations
Question: I am writing justification for having two TEMs in the same location rather one EM in two locations. I have a lot of reasons but just want to make sure I am not overlooking anything. Could I please get some opinions?
Thanks to all who help, Neil
Neil Medvitz Electron Microscopy Technical Director
==============================Original Headers============================== 11, 12 -- From zaluzec-at-microscopy.com Thu Jun 14 19:18:48 2007 11, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5F0Im9L004972 11, 12 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 19:18:48 -0500 11, 12 -- Mime-Version: 1.0 11, 12 -- Message-Id: {p06240805c2978b531766-at-[206.69.208.22]} 11, 12 -- Date: Thu, 14 Jun 2007 19:18:47 -0500 11, 12 -- To: microscopy-at-microscopy.com 11, 12 -- From: nmedvitz-at-nephrocor.com (by way of MicroscopyListserver) 11, 12 -- Subject: viaWWW: Advantages of having two TEMs in the same location rather 11, 12 -- than having one EM in two locations 11, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
We are renovating our lab to install an FEI Tecnai G2 20TEM. As we think about the electrical needs, we wonder about the necessity of backing the system with an uninterrupted power supply and generator. My understanding is that in the event of a power outage, the system will shut down, the pneumatic valves will close and the microscope will survive. But I wonder about the support computers. I am asking for the advice of others in how they have addressed UPS for their modern TEM systems.
Many thanks,
Doug
Douglas R. Keene Assistant Investigator Micro-Imaging Center Shriners Hospital for Children 3102 S.W. Sam Jackson Park Road Portland, Oregon 97239 503-221-3434 drk-at-shcc.org
==============================Original Headers============================== 7, 22 -- From drk-at-SHCC.org Thu Jun 14 19:59:23 2007 7, 22 -- Received: from mail.SHCC.org (mail.shcc.org [64.213.211.202]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5F0xNPn017267 7, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 19:59:23 -0500 7, 22 -- Received: from DRK2 (drk2.research.shcc.org [64.213.211.62]) 7, 22 -- by mail.SHCC.org (PMDF V6.3 #31473) 7, 22 -- with ESMTPA id {0JJN0052CJY49G-at-mail.SHCC.org} for Microscopy-at-Microscopy.Com; 7, 22 -- Thu, 14 Jun 2007 17:56:29 -0700 (PDT) 7, 22 -- Date: Thu, 14 Jun 2007 18:00:51 -0700 7, 22 -- From: Doug Keene {drk-at-SHCC.org} 7, 22 -- Subject: UPS for TEMs 7, 22 -- Sender: drk-at-SHCC.org 7, 22 -- To: Microscopy-at-Microscopy.Com 7, 22 -- Reply-to: drk-at-SHCC.org 7, 22 -- Message-id: {0JJN0052DJY49G-at-mail.SHCC.org} 7, 22 -- Organization: Shriners Hospitals for Children 7, 22 -- MIME-version: 1.0 7, 22 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 7, 22 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 7, 22 -- Content-type: text/plain; charset=us-ascii 7, 22 -- Content-transfer-encoding: 7BIT 7, 22 -- Thread-Index: Aceu6J48gStBi823SVWXipCrNp21ww== ==============================End of - Headers==============================
If power goes off, your gun goes off. Of course, if it is the same as an FESEM.
My solution is two 8KVA dual redundant Liebert nFinity dual conversion UPS units. Split the load between the two units. The SEM is on one unit while the EDS, PCs, chiller and other stuff are on the other unit. With redundant converters and controllers, an 8KVA unit does 4KVA with half of a unit. If one portion fails, the other half works. Fix the failed unit and back to 100% redundant.
Bad power is a big problem here in CA. Dual conversion is a huge benefit of these UPS. Steady, constant and always there. With full set of batteries, I have about 280 minutes of UPS backup for each UPS. each unit sees about 22% load.
Full complement of batteries (no expansion unit), and redundant controllers runs about $10K per unit. They are rock solid. For about $300 each, you can hook them to a LAN and monitor what is going on with power. You will be surprised. Or, manually view the event log.
Liebert makes higher capacity units but these work for my application.
gary g.
At 05:00 PM 6/14/2007, you wrote:
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==============================Original Headers============================== 13, 20 -- From gary-at-gaugler.com Fri Jun 15 00:08:06 2007 13, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5F585ce000483 13, 20 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 00:08:06 -0500 13, 20 -- Message-Id: {200706150508.l5F585ce000483-at-ns.microscopy.com} 13, 20 -- Received: (qmail 1410 invoked from network); 14 Jun 2007 22:08:05 -0700 13, 20 -- Received: by simscan 1.1.0 ppid: 1407, pid: 1408, t: 0.1095s 13, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 13, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 13, 20 -- by qsmtp3 with SMTP; 14 Jun 2007 22:08:05 -0700 13, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 20 -- Date: Thu, 14 Jun 2007 22:08:01 -0800 13, 20 -- To: drk-at-SHCC.org 13, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 13, 20 -- Subject: Re: [Microscopy] UPS for TEMs 13, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 13, 20 -- In-Reply-To: {200706150100.l5F10oO5020068-at-ns.microscopy.com} 13, 20 -- References: {200706150100.l5F10oO5020068-at-ns.microscopy.com} 13, 20 -- Mime-Version: 1.0 13, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-730C7E22 ==============================End of - Headers==============================
I agrees with the different comments about Pd Mz line overlapping on Carbon K. I had the same stuff with new polished and ion etched Pt standards, where I found that intrusive carbon... which was Pd-N line ! And my software don't have the N lines tabulated ! I had too the probleme with the Pd-M in Pd/Fe alloys.
Added to the overlapping probleme, in some situation one have catalysis effect on carbon cracking. The carbon peak comes really from carbon, but groes during the spectrum acquisition ! In such case you should see these famous hated black squares after the analysis...
About the uncoated sample, a collegue now retired said me never to use plastic bags or plasctic boxes for sample storage. Most plastics outgas solvent continusly and polluate all the surfaces with solvent. He used only glass ware. One need only a monolayer of hydrocarbon to have some contamination ! People who make Auger spectroscopy know that one must clean away the carbon before one see something else !
I've made some test, and my conclusion is that in most cases, the sample and the sample hodler bring with them the major source of contamination. The second source is the rotary-vane pump, and the last is the diff pump.
The two ways to limit contamination are first to use a lN2 cold trap very near of the sample, in front of the OL, or beside , or around it (one must have a fast entry lock), and second to put all parts which comes to air, the holder and the sample in a plasma cleaner, before putting them in the SEM (one must have much monney to buy one !). A simple glow discharge in air can be suffisant, but is not very reproductible.
About the Supra configuration, just a coment : as we bought our first FE-SEM, I saw in the test round that the Supra (in that time it was a 1530) was the SEM which had the most contamination from the five SEM we have seen. It was one raison for an other choice. Now we have bought a Supra 40 since a few month, for E-beam lithography, a application where one know that contamination cannot be avoided (one put in samples with a fresh coating of PMMA or other durty stuffs !), I have seen that, before performing any litho work, that contamination question was still the same. People from Leo/Zeiss say that the good performences of their SE-decteor reveal very thin contamination levels. My thought is that the vacuum demand isn't high enough. One cannot vent to air inocently a vaccum chamber with a so big specific surface and a turbo pump at each sample change without consequences. With the time one catch organic contamination from the environnement in the SEM itself. This can be one more source of contamination.
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
gary-at-gaugler.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Greetings all--I am seeking input on what appears } to be Carbon contamination. Here is the situation. } } Take a Pella 16111-9 stub out of bag and put on } holder. Take another stub out of bag and sputter } coat with Pd and put on holder. Do EDS on both. } } un-coated: } wt% at% } C 7.5 15 } O 4.5 7 } Al 88 78 } } coated: } C 23 38 } O 6.5 8 } Al 71 53 } } SEM is Zeiss Supra 55VP with Edwards XDS10 dry } scroll pump and turbo. Coater is Denton Desk IV } with Edwards XDS5 and turbo. } } The goal of using non-oil pumps was to reduce hydrocarbon } contamination. So, where is the C coming from? Nothing } has been done to the scroll pumps since new. There are } kits for repairing them but when is this necessary and } what would indicate that it be done? Would high C } be an indicator? } } I'm stumped on this one. } } Any ideas? } } gary g. } } } ==============================Original Headers============================== } 10, 17 -- From gary-at-gaugler.com Thu Jun 14 11:54:47 2007 } 10, 17 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) } 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5EGslUC027192 } 10, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 11:54:47 -0500 } 10, 17 -- Message-Id: {200706141654.l5EGslUC027192-at-ns.microscopy.com} } 10, 17 -- Received: (qmail 15711 invoked from network); 14 Jun 2007 09:54:47 -0700 } 10, 17 -- Received: by simscan 1.1.0 ppid: 15708, pid: 15709, t: 0.1452s } 10, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 } 10, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) } 10, 17 -- by qsmtp4 with SMTP; 14 Jun 2007 09:54:46 -0700 } 10, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 10, 17 -- Date: Thu, 14 Jun 2007 09:54:46 -0800 } 10, 17 -- To: MSA listserver {microscopy-at-microscopy.com} } 10, 17 -- From: Gary Gaugler {gary-at-gaugler.com} } 10, 17 -- Subject: Carbon "contamination" } 10, 17 -- Mime-Version: 1.0 } 10, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-20B34597 } ==============================End of - Headers============================== }
==============================Original Headers============================== 13, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Jun 15 02:33:10 2007 13, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.157]) 13, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5F7XArb014857 13, 29 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 02:33:10 -0500 13, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 13, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id l5F7X8Kj011806 13, 29 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 09:33:09 +0200 (CEST) 13, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 13, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 69E867E40A1 13, 29 -- for {Microscopy-at-Microscopy.Com} ; Fri, 15 Jun 2007 09:32:26 +0200 (CEST) 13, 29 -- Message-ID: {467240A5.7060607-at-ipcms.u-strasbg.fr} 13, 29 -- Date: Fri, 15 Jun 2007 09:32:53 +0200 13, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 13, 29 -- User-Agent: Thunderbird 1.5.0.12 (X11/20070604) 13, 29 -- MIME-Version: 1.0 13, 29 -- To: Microscopy-at-microscopy.com 13, 29 -- Subject: Re: [Microscopy] Carbon "contamination" 13, 29 -- References: {200706141700.l5EH0QJH003815-at-ns.microscopy.com} 13, 29 -- In-Reply-To: {200706141700.l5EH0QJH003815-at-ns.microscopy.com} 13, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 29 -- Content-Transfer-Encoding: 8bit 13, 29 -- X-IPCMS-MailScanner: Found to be clean 13, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 13, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.157]); Fri, 15 Jun 2007 09:33:09 +0200 (CEST) 13, 29 -- X-Virus-Scanned: ClamAV 0.88.7/3424/Fri Jun 15 03:30:29 2007 on mr7.u-strasbg.fr 13, 29 -- X-Virus-Status: Clean 13, 29 -- X-Spam-Status: No, score=-0.3 required=5.0 tests=AWL autolearn=disabled 13, 29 -- version=3.1.8 13, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr7.u-strasbg.fr ==============================End of - Headers==============================
In my former mail, in answer to Gary's question, I have said somthing from the use of the plasma cleaner. I take the opportunity to comme with a new question.
Did some one still build a plasma cleaner in the SEM itself, or better in the fast entry lock from the SEM. This would be the most drasctic way to avoid the contamination coming with the sample/sample holder.
If some has tried, or know from some tests...
Thanks
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Dear Jaques, have a look at http://www.semclean.com It seems to work greatly and is used - to my knowledge - by some major sem manufacturer - in Europe...
Best regards, Stefan Diller
----- Original Message ----- X-from: {jacques.faerber-at-ipcms.u-strasbg.fr} To: {stefan.diller-at-t-online.de} Sent: Friday, June 15, 2007 9:44 AM
Hi all
Receving just my mail from this morning, I see that I that I have made an error in the first paragraph. It is the Pt-N line and not the Pd-N line, which interfer, like the Pd-M.
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Yes, a plasma cleaner for an EM Chamber is a commerical product. There have been several papers written on this from both the SEM and TEM standpoint.
You should look at the following WWW site from XEI Scientific for the SEM info. They have a number of papers on-line to download.
http://www.evactron.com/
Disclaimer: Argonne National Lab, my daytime employer holds the basic patent on this technology for EM applications and licenses it to a number of commerical organizations, XEI being one of them.
Nestor Your Friendly Neighborhood SysOp.
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Hi all
In my former mail, in answer to Gary's question, I have said somthing from the use of the plasma cleaner. I take the opportunity to comme with a new question.
Did some one still build a plasma cleaner in the SEM itself, or better in the fast entry lock from the SEM. This would be the most drasctic way to avoid the contamination coming with the sample/sample holder.
If some has tried, or know from some tests...
Thanks
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des MatÈriaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Numerical Aperture: This is a critical value that indicates the light acceptance angle, which in turn determines the light gathering power, the resolving power, and depth of field of the objective.
Thus the higher the resolving power (NA) of an objective, the lower the depth of field (and so working distance). So a high NA objective may not suite all applications. Indeed a typical high magnification objective focus point will only just penetrate through a standard 0.17mm glass coverslip. An air objective will have a far greater (relatively) depth of field and thus a far longer working distance, particularly an LD 'long working distance' variety - but the image quality will be noticeably poorer (not a problem though if you can't see squat with your high NA short working distance oil immersion lens). This is all to do with airey disks and stuff, [and also a lot to do with the price of the objective and how well it's made]. Image contrast, as well as resolution, is also an important consideration here.
'Working distance' correction Collar
An adjustment [correction] collar for cover glass thickness (not NA) is sometimes provided on high-NA microscope objective lenses. Rotation of the collar adjusts the height of certain lens elements in the objective lens to compensate for variations in coverslip thickness or immersion media. Thus it provides an adjustable working distance. At high NAs, even a small deviation of the coverslip thickness (by as little as a few micrometers in some cases), or refractive index of the immersion medium from the designated standard, can introduce significant aberrations.
Variable NA collar
Other objectives specifically designed for transmitted light fluorescence and darkfield imaging are sometimes equipped with an internal iris diaphragm that allows for adjustment of the effective numerical aperture [NA]. A 60x apochromat objective can have a numerical aperture of 1.4, one of the highest attainable in modern microscopes using immersion oil as an imaging medium.
Variable Numerical Aperture Objectives: Specimens with unusually high fluorescence quantum yields and/or very bright darkfield specimens often induce image flare by light emitted from areas outside the focal plane. To compensate for this artefact, manufacturers offer high numerical aperture objectives that are equipped with an internal iris diaphragm to increase image contrast during photomicrography or digital imaging. Opening or closing the iris diaphragm determines the size of the objective rear aperture yielding a variable numerical aperture range between 0.5 and the objective's upper limit (up to 1.35-1.4 with apochromatic objectives). Although iris diaphragms were once utilized in a wide variety of objective designs, modern variable numerical aperture objectives are usually at the high end (60x to 150x) of the magnification range.
A variable NA collar on an oil immersion thus help with contrast enhancement when set to low NA, but it's not going to help it in any way achieve the long working distance of a dry LD objective of the same magnification. I must admit that I don't bother too much with the physics equations when looking down the microscope, as any decent microscope rep should be able provide you with a set of objectives to assess which one suites your needs best before you buy. Typically you would go for the highest NA you can for the given working distance required. Manufacturer's websites provide the objectives working distance (in mm) and NA info. Modern ultra-high NA objectives are required for TIRF applications and hi-res DIC contrast enhancement.
To achieve higher NA than 0.95 for objectives they have to be used with immersion media between front lens and specimen. Oil or water is mostly used for that purpose. Because objectives have to be specially designed for this, the type of immersion media is always mentioned on the objective like on the UPlanFLN 60x/1.25 Oil Iris objective. This objective needs oil as an immersion media and can not produce good image quality without it.
High NA objectives also collect more light and if you compare two objectives with fluorescent samples, the high NA objective should produce a noticeably brighter (and preferable) image. Again though the high NA objective is generally far more expensive and it's better attention to quality optics/coatings will also be factor in it's superior performance. An objective also needs to be PLAN Apochromat and fluorescence friendly for quality fluorophore imaging.
The only problem with using our variable NA collar objectives here is that we use inverted optics and have no idea where the NA collar is actually set [e.g. if it's moved accidentally or the previous user has adjusted it]. I do put a chart on the wall telling users which way to turn the NA collar for highest NA (viewed from above), but I'm not sure all users bother with this. I have noticed slight changes in um/pixel calibration (less than 10% difference, and it's linear) between the two NA extremes on some objectives.
To be told more than you probably want to know about resolving power and NA see:
----------------------- Dr Keith J Morris [Formerly] Imaging Facilities Manager Cell Biology Institute of Ophthalmology UCL, London EC1V 9EL
-----Original Message----- X-from: david.knecht-at-uconn.edu [mailto:david.knecht-at-uconn.edu] Sent: 14 June 2007 18:44 To: keith.morris-at-ucl.ac.uk
One can buy a 40-100x oil objective with an iris diaphram. What is the theoretical difference in performance between imaging with a low NA dry objective (.6NA for example) and an equivalent magnification, high NA oil objective with the iris partially closed to reduce the NA to .6? Should they be similar? Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 5, 19 -- From david.knecht-at-uconn.edu Thu Jun 14 12:40:46 2007 5, 19 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EHekrM018923 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 12:40:46 -0500 5, 19 -- Received: from [137.99.46.174] (d46h174.public.uconn.edu [137.99.46.174]) 5, 19 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id l5EHeffX009795 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 13:40:41 -0400 5, 19 -- Mime-Version: 1.0 (Apple Message framework v752.3) 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- Message-Id: {D06FF75E-9614-4D26-BA77-9425A50AFCAD-at-uconn.edu} 5, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 19 -- To: microscopy {microscopy-at-microscopy.com} 5, 19 -- From: David Knecht {david.knecht-at-uconn.edu} 5, 19 -- Subject: objective NA 5, 19 -- Date: Thu, 14 Jun 2007 13:40:34 -0400 5, 19 -- X-Mailer: Apple Mail (2.752.3) 5, 19 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information. 5, 19 -- X-UConn-MailScanner: Found to be clean 5, 19 -- X-UConn-MailScanner-SpamCheck: ==============================End of - Headers==============================
==============================Original Headers============================== 35, 24 -- From keith.morris-at-ucl.ac.uk Fri Jun 15 08:19:33 2007 35, 24 -- Received: from smtp3.global.net.uk (smtp3.global.net.uk [80.189.92.91]) 35, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5FDJWuD015640 35, 24 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 08:19:33 -0500 35, 24 -- Received: from 190.79.rb4.adsl.brightview.com ([80.189.79.190] helo=loungepc) 35, 24 -- by smtp3.global.net.uk with esmtp (Exim 4.66 (FreeBSD)) 35, 24 -- (envelope-from {keith.morris-at-ucl.ac.uk} ) 35, 24 -- id 1HzBi5-000Ls6-Lq 35, 24 -- for microscopy-at-microscopy.com; Fri, 15 Jun 2007 14:19:31 +0100 35, 24 -- From: "Keith Morris" {keith.morris-at-ucl.ac.uk} 35, 24 -- To: {microscopy-at-microscopy.com} 35, 24 -- References: {200706141744.l5EHiHGX025348-at-ns.microscopy.com} 35, 24 -- Subject: RE: [Microscopy] objective NA 35, 24 -- Date: Fri, 15 Jun 2007 14:19:27 +0100 35, 24 -- Message-ID: {000001c7af4f$cede49a0$0301a8c0-at-loungepc} 35, 24 -- MIME-Version: 1.0 35, 24 -- Content-Type: text/plain; 35, 24 -- charset="us-ascii" 35, 24 -- Content-Transfer-Encoding: 7bit 35, 24 -- X-Mailer: Microsoft Office Outlook 11 35, 24 -- Thread-Index: Aceuq65IN79W3iYhQV6BDQVD6ZJEJAAkxKsg 35, 24 -- In-Reply-To: {200706141744.l5EHiHGX025348-at-ns.microscopy.com} 35, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 35, 24 -- Authenticated-Sender: ==============================End of - Headers==============================
Only saw someone's response to this question last evening and did not get to respond immediately, but Gary has really hit a major point. It is more than just protecting against the power outage. Most - if not all - microscopes have all sorts of emergency backups which go in place to prevent any real damage. Power outages are not common, butd they are major irritations when they occur. A real problem is spikes in power - the dirty power Gary refers to.
When I took over my current position it took me 18 months to figure out that the separate and isolated power source for my lab was neither separate nor isolated. The department had even put in (before me) a nice Barrie anti vibration system - works great, didn't stop the instability problems. We finally figured out what was happening when it was noticed that the aberrations in beam and focus occurred whenever someone was doing gel electrophoresis in a bank of labs in the next hallway. When we put a monitor on the power in line we found over 130 spikes in power in excess of 5% over the normal 12hour daytime period.
Fluctuations in power - spikes - do occur regularly. Not just in California, but in Winnipeg, Montreal, London, New York, Tokyo, etc. Surge protectors kick in after the first cycle of a spike. As a result, they do not protect against spikes with your computers and other 'not a microscope' equipment, they . Other than the glowing little light and giving you 5 plugs from 1 they probably do nothing to help.
Damage occurs in the first spike. This is true of the EM also. Dirty lines mean pops in and out of focus. If you've ever seen one, you know what this is. Imagine the fresnel fringe popping in and out concentrically while you look at the image you are trying to see. It may not bother you when you look at a section at 10,000, but it will drive you nuts when looking at a virus at 100,000+.
I didn't put in a UPS at the time. 25 years ago the unit would have cost } 10,000 and needed it's own room. But when we moved a microscope recently during a lab consolidation I built into the cost $2500 to put in a UPS. The best time to get things from administrators is when you are building a new lab, moving, renovating, or putting in a new microscope. Even if you have to spend Gary's $10-12,000US to protect several microscopes, or my $2,200C to protect one microscope, it is worth it.
==============================Original Headers============================== 7, 20 -- From paul_hazelton-at-umanitoba.ca Fri Jun 15 08:40:21 2007 7, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5FDeLei027646 7, 20 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 08:40:21 -0500 7, 20 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 7, 20 -- (authenticated bits=0) 7, 20 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id l5FDeJTc001912; 7, 20 -- Fri, 15 Jun 2007 08:40:19 -0500 (CDT) 7, 20 -- Message-ID: {46729645.6020801-at-umanitoba.ca} 7, 20 -- Date: Fri, 15 Jun 2007 08:38:13 -0500 7, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 7, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 7, 20 -- X-Accept-Language: en-us, en 7, 20 -- MIME-Version: 1.0 7, 20 -- To: gary-at-gaugler.com 7, 20 -- Subject: Re: [Microscopy] Re: UPS for TEMs 7, 20 -- References: {200706150510.l5F5ALIw003878-at-ns.microscopy.com} 7, 20 -- In-Reply-To: {200706150510.l5F5ALIw003878-at-ns.microscopy.com} 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have the same saw, functioning, and still have the original manual that came with it. So if you'd like, I can send you a copy.
However, I would suggest that you just call Buehler. I'm sure thay have a .pdf file with the manual and would send it to you. Buehler has sent me manuals, calibration data, all kinds of information on older equipment that they manufactured. They are really top notch in this regard, at least that has been my experience.
dj
On Thu, 14 Jun 2007, frah0010-at-umn.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Microscopists, } } Our lab has a Buehler IsoMet slow saw (model #11-1180, apparently } 1980s vintage) that has ceased functioning. I've tracked the problem } down to broken gear teeth on the shaft perpendicular to the motor's } drive shaft. Does anyone else have a dead or unused IsoMet that they } wouldn't mind someone cannibalizing? We can pay shipping and/or } modestly for the equipment/parts. We also don't have the } instructions or specifications anymore. If someone else does and } there are good part descriptions, I'd be interested in a fax or scan } of that page to help track down a replacement part. Thanks in advance! } } Cheers, } Ellery } } -------------------- } Ellery E. Frahm } Research Fellow & Manager } Electron Microprobe Laboratory } University of Minnesota - Twin Cities } Department of Geology & Geophysics } Lab Website: http://probelab.geo.umn.edu } Personal Website: http://umn.edu/~frah0010 } } Please note: Sometimes the University's spam filters are over- } protective and reject wanted messages, especially from free or } overseas accounts. If your message is rejected or you are concerned } that you did not receive a reply from me, please feel free to try my } alternate email account: elleryfrahm-at-mac.com } } } ==============================Original Headers============================== } 6, 17 -- From frah0010-at-umn.edu Thu Jun 14 18:58:38 2007 } 6, 17 -- Received: from mta-w2.tc.umn.edu (mta-w2.tc.umn.edu [134.84.119.6]) } 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l5ENwcCp025383 } 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 18:58:38 } -0500 } 6, 17 -- Received: from [10.0.1.3] (c-75-72-182-252.hsd1.mn.comcast.net } [75.72.182.252]) } 6, 17 -- by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP } 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 18:58:38 } -0500 (CDT) } 6, 17 -- X-Umn-Remote-Mta: [N] c-75-72-182-252.hsd1.mn.comcast.net } [75.72.182.252] #+TS+AU+HN } 6, 17 -- Mime-Version: 1.0 (Apple Message framework v752.2) } 6, 17 -- Content-Transfer-Encoding: 7bit } 6, 17 -- Message-Id: {1862217B-D7B4-46CA-99B6-0819AFC25E13-at-umn.edu} } 6, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed } 6, 17 -- To: microscopy-at-microscopy.com } 6, 17 -- From: Ellery Frahm {frah0010-at-umn.edu} } 6, 17 -- Subject: Buehler IsoMet } 6, 17 -- Date: Thu, 14 Jun 2007 18:58:37 -0500 } 6, 17 -- X-Mailer: Apple Mail (2.752.2) } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 16 -- From dljones-at-bestweb.net Fri Jun 15 09:02:44 2007 7, 16 -- Received: from vms046pub.verizon.net (vms046pub.verizon.net [206.46.252.46]) 7, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5FE2iNB007254 7, 16 -- for {Microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 09:02:44 -0500 7, 16 -- Received: from localhost ([71.247.229.59]) 7, 16 -- by vms046.mailsrvcs.net (Sun Java System Messaging Server 6.2-6.01 (built Apr 7, 16 -- 3 2006)) with ESMTPA id {0JJO001NEKBXQX74-at-vms046.mailsrvcs.net} for 7, 16 -- Microscopy-at-microscopy.com; Fri, 15 Jun 2007 09:02:28 -0500 (CDT) 7, 16 -- Date: Fri, 15 Jun 2007 10:03:41 -0400 (Eastern Daylight Time) 7, 16 -- From: "David L. Jones" {dljones-at-bestweb.net} 7, 16 -- Subject: Re: [Microscopy] Buehler IsoMet 7, 16 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 7, 16 -- To: Microscopy-at-microscopy.com 7, 16 -- Message-id: {Pine.WNT.4.64.0706151003230.3116-at-H-F1} 7, 16 -- MIME-version: 1.0 7, 16 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Lots of useful information in the responses, but let me clarify the question. A colleague is using his stopped down 40x oil objective in order to visualize vesicles throughout the volume of the cell in one image rather than confocal sectioning. I am trying to understand why it works and whether it would work equivalently with a dry objective. Obviously the light cone from a 1.4 NA 40x oil objective (whether iris is closed or not) and a 40x long working distance dry objective are different. The depth of field varies with numerical aperture, so a 40x 1.4 oil immersion lens, should inherently have a shallower depth of focus. Therefore, it makes no intuitive sense to me that you would #1-increase the depth of focus, and #2- get the same results with a 40x oil 1.4 in which you stop down the iris to . 5NA, as a .5NA LWD dry lens. The excitation spot profile of the 40x oil objective is not going to enlarge as you stop down the iris with the same input light path. So I am trying to understand how the focal depth/resolution/xyz profile of the excitation/emission etc. is for fluorescence in comparing those two situations. Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
On Jun 15, 2007, at 9:19 AM, keith.morris-at-ucl.ac.uk wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi David, } } Numerical Aperture: This is a critical value that indicates the light } acceptance angle, which in turn determines the light gathering } power, the } resolving power, and depth of field of the objective. } } Thus the higher the resolving power (NA) of an objective, the lower } the } depth of field (and so working distance). So a high NA objective } may not } suite all applications. Indeed a typical high magnification } objective focus } point will only just penetrate through a standard 0.17mm glass } coverslip. An } air objective will have a far greater (relatively) depth of field } and thus a } far longer working distance, particularly an LD 'long working } distance' } variety - but the image quality will be noticeably poorer (not a } problem } though if you can't see squat with your high NA short working } distance oil } immersion lens). This is all to do with airey disks and stuff, [and } also a } lot to do with the price of the objective and how well it's made]. } Image } contrast, as well as resolution, is also an important consideration } here. } } } 'Working distance' correction Collar } } An adjustment [correction] collar for cover glass thickness (not } NA) is } sometimes provided on high-NA microscope objective lenses. Rotation } of the } collar adjusts the height of certain lens elements in the objective } lens to } compensate for variations in coverslip thickness or immersion } media. Thus it } provides an adjustable working distance. At high NAs, even a small } deviation } of the coverslip thickness (by as little as a few micrometers in some } cases), or refractive index of the immersion medium from the } designated } standard, can introduce significant aberrations. } } } Variable NA collar } } Other objectives specifically designed for transmitted light } fluorescence } and darkfield imaging are sometimes equipped with an internal iris } diaphragm } that allows for adjustment of the effective numerical aperture } [NA]. A 60x } apochromat objective can have a numerical aperture of 1.4, one of the } highest attainable in modern microscopes using immersion oil as an } imaging } medium. } } Variable Numerical Aperture Objectives: Specimens with unusually high } fluorescence quantum yields and/or very bright darkfield specimens } often } induce image flare by light emitted from areas outside the focal } plane. To } compensate for this artefact, manufacturers offer high numerical } aperture } objectives that are equipped with an internal iris diaphragm to } increase } image contrast during photomicrography or digital imaging. Opening or } closing the iris diaphragm determines the size of the objective rear } aperture yielding a variable numerical aperture range between 0.5 } and the } objective's upper limit (up to 1.35-1.4 with apochromatic objectives). } Although iris diaphragms were once utilized in a wide variety of } objective } designs, modern variable numerical aperture objectives are usually } at the } high end (60x to 150x) of the magnification range. } } A variable NA collar on an oil immersion thus help with contrast } enhancement } when set to low NA, but it's not going to help it in any way } achieve the } long working distance of a dry LD objective of the same } magnification. I } must admit that I don't bother too much with the physics equations } when } looking down the microscope, as any decent microscope rep should be } able } provide you with a set of objectives to assess which one suites } your needs } best before you buy. Typically you would go for the highest NA you } can for } the given working distance required. Manufacturer's websites } provide the } objectives working distance (in mm) and NA info. Modern ultra-high NA } objectives are required for TIRF applications and hi-res DIC contrast } enhancement. } } To achieve higher NA than 0.95 for objectives they have to be used } with } immersion media between front lens and specimen. Oil or water is } mostly used } for that purpose. Because objectives have to be specially designed } for this, } the type of immersion media is always mentioned on the objective } like on the } UPlanFLN 60x/1.25 Oil Iris objective. This objective needs oil as an } immersion media and can not produce good image quality without it. } } High NA objectives also collect more light and if you compare two } objectives } with fluorescent samples, the high NA objective should produce a } noticeably } brighter (and preferable) image. Again though the high NA objective is } generally far more expensive and it's better attention to quality } optics/coatings will also be factor in it's superior performance. An } objective also needs to be PLAN Apochromat and fluorescence } friendly for } quality fluorophore imaging. } } The only problem with using our variable NA collar objectives here } is that } we use inverted optics and have no idea where the NA collar is } actually set } [e.g. if it's moved accidentally or the previous user has adjusted } it]. I do } put a chart on the wall telling users which way to turn the NA } collar for } highest NA (viewed from above), but I'm not sure all users bother } with this. } I have noticed slight changes in um/pixel calibration (less than 10% } difference, and it's linear) between the two NA extremes on some } objectives. } } } To be told more than you probably want to know about resolving } power and NA } see: } } http://www.olympusmicro.com/primer/anatomy/specialobjectives.html } http://www.microscopy.olympus.eu/microscopes/About_Microscopy_7436.htm } [The resolving power] } http://www.charfac.umn.edu/glossary/c.html } http://www.oznet.ksu.edu/ed_agron645/lab/microscope_use.htm } http://www.olympusconfocal.com/theory/confocalobjectives.html } http://www.microscopyu.com/tutorials/java/objectives/nuaperture/ } index.html } http://www.microscopyu.com/articles/optics/objectivespecs.html } http://www.micro.magnet.fsu.edu/primer/java/aberrations/ } slipcorrection/index } .html } } Regards } } Keith } } ----------------------- } Dr Keith J Morris } [Formerly] Imaging Facilities Manager } Cell Biology } Institute of Ophthalmology } UCL, London EC1V 9EL } } } } } -----Original Message----- } X-from: david.knecht-at-uconn.edu [mailto:david.knecht-at-uconn.edu] } Sent: 14 June 2007 18:44 } To: keith.morris-at-ucl.ac.uk } Subject: [Microscopy] objective NA } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } One can buy a 40-100x oil objective with an iris diaphram. What is } the theoretical difference in performance between imaging with a low } NA dry objective (.6NA for example) and an equivalent magnification, } high NA oil objective with the iris partially closed to reduce the NA } to .6? Should they be similar? Thanks- Dave } } } Dr. David Knecht } Department of Molecular and Cell Biology } Co-head Flow Cytometry and Confocal Microscopy Facility } U-3125 } 91 N. Eagleville Rd. } University of Connecticut } Storrs, CT 06269 } 860-486-2200 } 860-486-4331 (fax) } } } } ==============================Original } Headers============================== } 5, 19 -- From david.knecht-at-uconn.edu Thu Jun 14 12:40:46 2007 } 5, 19 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu } [137.99.25.204]) } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l5EHekrM018923 } 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 12:40:46 } -0500 } 5, 19 -- Received: from [137.99.46.174] (d46h174.public.uconn.edu } [137.99.46.174]) } 5, 19 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id } l5EHeffX009795 } 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 13:40:41 } -0400 } 5, 19 -- Mime-Version: 1.0 (Apple Message framework v752.3) } 5, 19 -- Content-Transfer-Encoding: 7bit } 5, 19 -- Message-Id: {D06FF75E-9614-4D26-BA77-9425A50AFCAD-at-uconn.edu} } 5, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; } format=flowed } 5, 19 -- To: microscopy {microscopy-at-microscopy.com} } 5, 19 -- From: David Knecht {david.knecht-at-uconn.edu} } 5, 19 -- Subject: objective NA } 5, 19 -- Date: Thu, 14 Jun 2007 13:40:34 -0400 } 5, 19 -- X-Mailer: Apple Mail (2.752.3) } 5, 19 -- X-UConn-MailScanner-Information: Contact UConn Help Desk } 860-486-4357 for more information. } 5, 19 -- X-UConn-MailScanner: Found to be clean } 5, 19 -- X-UConn-MailScanner-SpamCheck: } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 35, 24 -- From keith.morris-at-ucl.ac.uk Fri Jun 15 08:19:33 2007 } 35, 24 -- Received: from smtp3.global.net.uk (smtp3.global.net.uk } [80.189.92.91]) } 35, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l5FDJWuD015640 } 35, 24 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 } 08:19:33 -0500 } 35, 24 -- Received: from 190.79.rb4.adsl.brightview.com } ([80.189.79.190] helo=loungepc) } 35, 24 -- by smtp3.global.net.uk with esmtp (Exim 4.66 (FreeBSD)) } 35, 24 -- (envelope-from {keith.morris-at-ucl.ac.uk} ) } 35, 24 -- id 1HzBi5-000Ls6-Lq } 35, 24 -- for microscopy-at-microscopy.com; Fri, 15 Jun 2007 14:19:31 } +0100 } 35, 24 -- From: "Keith Morris" {keith.morris-at-ucl.ac.uk} } 35, 24 -- To: {microscopy-at-microscopy.com} } 35, 24 -- References: {200706141744.l5EHiHGX025348-at-ns.microscopy.com} } 35, 24 -- Subject: RE: [Microscopy] objective NA } 35, 24 -- Date: Fri, 15 Jun 2007 14:19:27 +0100 } 35, 24 -- Message-ID: {000001c7af4f$cede49a0$0301a8c0-at-loungepc} } 35, 24 -- MIME-Version: 1.0 } 35, 24 -- Content-Type: text/plain; } 35, 24 -- charset="us-ascii" } 35, 24 -- Content-Transfer-Encoding: 7bit } 35, 24 -- X-Mailer: Microsoft Office Outlook 11 } 35, 24 -- Thread-Index: Aceuq65IN79W3iYhQV6BDQVD6ZJEJAAkxKsg } 35, 24 -- In-Reply-To: {200706141744.l5EHiHGX025348-at-ns.microscopy.com} } 35, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 } 35, 24 -- Authenticated-Sender: } ==============================End of - } Headers==============================
==============================Original Headers============================== 6, 21 -- From david.knecht-at-uconn.edu Fri Jun 15 09:20:25 2007 6, 21 -- Received: from mail1.uits.uconn.edu (mail1.uits.uconn.edu [137.99.25.203]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5FEKPs1019064 6, 21 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 09:20:25 -0500 6, 21 -- Received: from [137.99.46.174] (d46h174.public.uconn.edu [137.99.46.174]) 6, 21 -- by mail1.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id l5FEKKOK018997 6, 21 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 10:20:20 -0400 6, 21 -- Mime-Version: 1.0 (Apple Message framework v752.3) 6, 21 -- In-Reply-To: {200706151319.l5FDJhjN015904-at-ns.microscopy.com} 6, 21 -- References: {200706151319.l5FDJhjN015904-at-ns.microscopy.com} 6, 21 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 21 -- Message-Id: {CAAE52D2-2A0E-419D-B0CD-A8D66B18E7C4-at-uconn.edu} 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- From: David Knecht {david.knecht-at-uconn.edu} 6, 21 -- Subject: Re: [Microscopy] RE: objective NA 6, 21 -- Date: Fri, 15 Jun 2007 10:20:18 -0400 6, 21 -- To: microscopy microscopy {microscopy-at-microscopy.com} 6, 21 -- X-Mailer: Apple Mail (2.752.3) 6, 21 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information. 6, 21 -- X-UConn-MailScanner: Found to be clean 6, 21 -- X-UConn-MailScanner-SpamCheck: ==============================End of - Headers==============================
The Inter/Micro 2007 conference, scheduled for July 9-13, 2007, is rapidly approaching!
Announcing the symposium's schedule for speakers:
2007 Schedule of Presentations
See http://www.mcri.org/2007ScheduleforSpeakers.pdf
Thursday Workshop:
'Working with Living Cells - Triumphs, Tribulations and Tragedies', Jeremy Pickett-Heaps of the School of Botany, University of Melbourne, & Brian J. Ford of Gonville & Caius College, Cambridge University.
Friday Workshop: 'Airborne & Settled Dust Particle Identification Workshop', Andrew A. Havics, pH2 LLC., Randy Boltin, MVA Scientific Consultants, & John D. Shane, Ph.D., PRO-LAB/SSPTM, Inc.
Tony
Ps Disclaimer - I receive no profits from this although I'm teaching part of the one workshop on Friday.
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
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==============================Original Headers============================== 18, 25 -- From ph2-at-sprynet.com Fri Jun 15 10:42:18 2007 18, 25 -- Received: from elasmtp-junco.atl.sa.earthlink.net (elasmtp-junco.atl.sa.earthlink.net [209.86.89.63]) 18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5FFgIph000332 18, 25 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 10:42:18 -0500 18, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 18, 25 -- s=dk20050327; d=sprynet.com; 18, 25 -- b=AgXTNIysJslzNcLufRKv9PZRYEs9nvlp4k8MzwWDGtWA1uM91uusNoEdBV0+3Lf/; 18, 25 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-MimeOLE:Thread-Index:Message-ID:X-ELNK-Trace:X-Originating-IP; 18, 25 -- Received: from [75.61.18.94] (helo=user915fa8f284) 18, 25 -- by elasmtp-junco.atl.sa.earthlink.net with asmtp (Exim 4.34) 18, 25 -- id 1HzDwH-0001BO-Ko; Fri, 15 Jun 2007 11:42:17 -0400 18, 25 -- From: "Tony Havics" {ph2-at-sprynet.com} 18, 25 -- To: "Micrscopy Listserve" {microscopy-at-microscopy.com} 18, 25 -- Subject: Inter/Micro 2007 18, 25 -- Date: Fri, 15 Jun 2007 11:42:15 -0400 18, 25 -- MIME-Version: 1.0 18, 25 -- Content-Type: text/plain; 18, 25 -- charset="US-ASCII" 18, 25 -- Content-Transfer-Encoding: 7bit 18, 25 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 18, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 18, 25 -- Thread-Index: AcevY7+canxfNHe0RAukF4ZByfBm0A== 18, 25 -- Message-ID: {E1HzDwH-0001BO-Ko-at-elasmtp-junco.atl.sa.earthlink.net} 18, 25 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f967630eb5f2bc75373f5134b39e4fcc3e350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 18, 25 -- X-Originating-IP: 75.61.18.94 ==============================End of - Headers==============================
Yes... I should have amplified about the UPS units.
Disregarding outages (that's what the UPS are for), spikes and sags are bad and can have catastrophic effects on the equipment (SEM, TEM, PCs, etc.).
There are basically two types of UPS. One is a simple APC brand style that switches on an inverter when the input power fails. These units can include spike clamps but do not handle sags. The second type is double conversion. This is the best method. This type takes input AC and converts it to DC (usually around 240VDC) and inverts it to AC via sine wave driver and LARGE output transformer. This produces pure sine wave output voltage with about 3% stability and total protection from sags and spikes.
If the input power fails, the unit instantly switches from the rectified input AC--} DC to the battery bank. Perfect. Plus, the Liebert has redundancy options. In my Amray days, I used a pair of Toshiba 1400xl+ dual conversion UPS. They worked well but did not have redundancy. The problem with this is that when one failed, the whole system went down. US service support for the Toshiba units was bad. The Liebert units are US made and US supported. After almost two years of continuous operation, neither of the Lieberts has failed. For about $1K, extended warranty is available. This covers the electronics and the batteries. Each battery is actually a module itself and if the monitors think a battery is bad, a light comes on on the battery module and a message is posted about it.
Getting a good UPS at install is the way to go. For a fraction of the cost of the SEM or TEM and accessories, it is cheap insurance.
gary g.
At 05:41 AM 6/15/2007, you wrote:
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==============================Original Headers============================== 13, 20 -- From gary-at-gaugler.com Fri Jun 15 11:20:12 2007 13, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5FGKBYO012572 13, 20 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 11:20:11 -0500 13, 20 -- Message-Id: {200706151620.l5FGKBYO012572-at-ns.microscopy.com} 13, 20 -- Received: (qmail 19581 invoked from network); 15 Jun 2007 09:20:11 -0700 13, 20 -- Received: by simscan 1.1.0 ppid: 19578, pid: 19579, t: 0.1280s 13, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 13, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 13, 20 -- by qsmtp1 with SMTP; 15 Jun 2007 09:20:11 -0700 13, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 20 -- Date: Fri, 15 Jun 2007 09:20:07 -0800 13, 20 -- To: paul_hazelton-at-umanitoba.ca 13, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 13, 20 -- Subject: Re: [Microscopy] UPS for TEMs 13, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 13, 20 -- In-Reply-To: {200706151341.l5FDflDM030494-at-ns.microscopy.com} 13, 20 -- References: {200706151341.l5FDflDM030494-at-ns.microscopy.com} 13, 20 -- Mime-Version: 1.0 13, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-1CCC3885 ==============================End of - Headers==============================
On Jun 14, 2007, at 5:59 PM, drk-at-SHCC.org wrote:
} We are renovating our lab to install an FEI Tecnai G2 20TEM. As we } think } about the electrical needs, we wonder about the necessity of backing } the } system with an uninterrupted power supply and generator. My } understanding } is that in the event of a power outage, the system will shut down, the } pneumatic valves will close and the microscope will survive. But I } wonder } about the support computers. I am asking for the advice of others in } how } they have addressed UPS for their modern TEM systems. } Dear Doug, We have a T12 and a TF30H, and we only have a UPS on the 30. We have not had any power failures that affected the computers, but we did have a failure in the UPS (fortunately, no power failures during the several months it took to get the UPS repair booked and done). Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
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Title-Subject: [Filtered] SPM, AFM, STM In Undergraduate Education
Question: On June 26, 2007, Agilent Technologies will be hosting a free web-based seminar on the Integration of AFM in Undergraduate Education. Jayne Garno, an assistant professor of chemistry at Louisiana State University, is working to integrate scanning probe experiments into junior and senior undergraduate laboratory courses in analytical and physical chemistry to give students hands-on experience with molecular imaging. In this informative eSeminar, she will describe her progress. Following Garno's presentation, Dr. Song Xu, an applications scientist with Agilent Technologies, will discuss research-grade AFM instrumentation appropriate for use in undergraduate education. There will also be a Q&A session in which all online attendees are welcome to query the presenters. More information, and to register, can be found at http://nano.tm.agilent.com
I didn't get a copy of my reply in the in-box (you obviously did), so it's also attached to the bottom here. Essentially the NA iris works like the iris on the condenser and a camera (to the eye, I don't know about the optical physics).
Using a camera with a macro lens, stopping [closing] the camera iris down to F22 brings everything in the macro shot into focus (a big depth of field), but at the expense of image brightness and probably a little resolution - so you need a longer exposure time. You aren't actually moving the camera any closer to the specimen though, so the 'working distance' hasn't changed. Plus your point of focus is still in the same place (as the lenses/camera haven't moved). See http://www.cs.mtu.edu/~shene/DigiCam/User-Guide/950/depth-of-field.html.
Stopping the NA iris in the high mag oil objective down (to low NA) increases the contrast and probably the depth of field around the focus point, but no doubt at the expense of absolute resolution (hence the lower NA). Naturally high resolution is pig all use without any contrast though to actually see the specimen, hence the advantage of the iris. It probably won't affect the maximum working distance at all though (you are still under oil), other than perhaps a greater depth of focus around the area you are focussed on (you can't physically move the objective into the sample any more). To increase actual working distance you have move the internal lenses (or one anyway) up and down, using a Working Distance [WD] collar if fitted (I've never seen a variable NA iris and variable WD lens collar fitted together on one objective though).
The increased contrast and greater depth of field at the lower NA iris setting will bring out intra-cellular structures far better, and it works with DIC as well. We use a Leica 63x [1.4-0.6 variable NA] HCX PL APO oil objective, which cost around £5,000 I believe. Partly due to it being a flash well made expensive objective, this Leica 'blue' variable NA 63x oil objective's image quality will always be far better than that obtained our long working distance Olympus LCPLFL 60x air objective [NA 0.7], the image quality of which is relatively appalling - but of course it can see well into the gels we use, unlike the Leica high NA oil version, and so is our only choice for such things. The Olympus 60x also has a variable NA to adjust the focus depth and contrast around the point of focus, but image quality at either NA extreme is still quite poor compared to the Leica oil (but it has that long working distance and can be physically moved to focus far further into the specimen).
Well that’s how I see it anyway (and it must be true as another guy at a conference I was at said a similar thing to me once). Unfortunately I don't have access to these objectives any more, as I would like to investigate it further (that’s the fun of this list-server it generates interesting questions).
We generally use these variable NA 63x objectives with fluorescent samples and z-slices under a laser confocal, so I doubt we'd notice any depth of field improvements with the lower NA setting as we are sticking the centre of the focus anyway.
Regards
Keith
----------------------- Dr Keith J Morris [Formerly] Imaging Facilities Manager Cell Biology Institute of Ophthalmology UCL, London EC1V 9EL
Numerical Aperture: This is a critical value that indicates the light acceptance angle, which in turn determines the light gathering power, the resolving power, and depth of field of the objective.
Thus the higher the resolving power (NA) of an objective, the lower the depth of field (and so working distance). So a high NA objective may not suite all applications. Indeed a typical high magnification objective focus point will only just penetrate through a standard 0.17mm glass coverslip. An air objective will have a far greater (relatively) depth of field and thus a far longer working distance, particularly an LD 'long working distance' variety - but the image quality will be noticeably poorer (not a problem though if you can't see squat with your high NA short working distance oil immersion lens). This is all to do with airey disks and stuff, [and also a lot to do with the price of the objective and how well it's made]. Image contrast, as well as resolution, is also an important consideration here.
'Working distance' correction Collar
An adjustment [correction] collar for cover glass thickness (not NA) is sometimes provided on high-NA microscope objective lenses. Rotation of the collar adjusts the height of certain lens elements in the objective lens to compensate for variations in coverslip thickness or immersion media. Thus it provides an adjustable working distance. At high NAs, even a small deviation of the coverslip thickness (by as little as a few micrometers in some cases), or refractive index of the immersion medium from the designated standard, can introduce significant aberrations.
Variable NA collar
Other objectives specifically designed for transmitted light fluorescence and darkfield imaging are sometimes equipped with an internal iris diaphragm that allows for adjustment of the effective numerical aperture [NA]. A 60x apochromat objective can have a numerical aperture of 1.4, one of the highest attainable in modern microscopes using immersion oil as an imaging medium.
Variable Numerical Aperture Objectives: Specimens with unusually high fluorescence quantum yields and/or very bright darkfield specimens often induce image flare by light emitted from areas outside the focal plane. To compensate for this artefact, manufacturers offer high numerical aperture objectives that are equipped with an internal iris diaphragm to increase image contrast during photomicrography or digital imaging. Opening or closing the iris diaphragm determines the size of the objective rear aperture yielding a variable numerical aperture range between 0.5 and the objective's upper limit (up to 1.35-1.4 with apochromatic objectives). Although iris diaphragms were once utilized in a wide variety of objective designs, modern variable numerical aperture objectives are usually at the high end (60x to 150x) of the magnification range.
A variable NA collar on an oil immersion thus help with contrast enhancement when set to low NA, but it's not going to help it in any way achieve the long working distance of a dry LD objective of the same magnification. I must admit that I don't bother too much with the physics equations when looking down the microscope, as any decent microscope rep should be able provide you with a set of objectives to assess which one suites your needs best before you buy. Typically you would go for the highest NA you can for the given working distance required. Manufacturer's websites provide the objectives working distance (in mm) and NA info. Modern ultra-high NA objectives are required for TIRF applications and hi-res DIC contrast enhancement.
To achieve higher NA than 0.95 for objectives they have to be used with immersion media between front lens and specimen. Oil or water is mostly used for that purpose. Because objectives have to be specially designed for this, the type of immersion media is always mentioned on the objective like on the UPlanFLN 60x/1.25 Oil Iris objective. This objective needs oil as an immersion media and can not produce good image quality without it.
High NA objectives also collect more light and if you compare two objectives with fluorescent samples, the high NA objective should produce a noticeably brighter (and preferable) image. Again though the high NA objective is generally far more expensive and it's better attention to quality optics/coatings will also be factor in it's superior performance. An objective also needs to be PLAN Apochromat and fluorescence friendly for quality fluorophore imaging.
The only problem with using our variable NA collar objectives here is that we use inverted optics and have no idea where the NA collar is actually set [e.g. if it's moved accidentally or the previous user has adjusted it]. I do put a chart on the wall telling users which way to turn the NA collar for highest NA (viewed from above), but I'm not sure all users bother with this. I have noticed slight changes in um/pixel calibration (less than 10% difference, and it's linear) between the two NA extremes on some objectives.
To be told more than you probably want to know about resolving power and NA see:
----------------------- Dr Keith J Morris [Formerly] Imaging Facilities Manager Cell Biology Institute of Ophthalmology UCL, London EC1V 9EL
-----Original Message----- X-from: david.knecht-at-uconn.edu [mailto:david.knecht-at-uconn.edu] Sent: 15 June 2007 15:25 To: keith.morris-at-ucl.ac.uk
Lots of useful information in the responses, but let me clarify the question. A colleague is using his stopped down 40x oil objective in order to visualize vesicles throughout the volume of the cell in one image rather than confocal sectioning. I am trying to understand why it works and whether it would work equivalently with a dry objective. Obviously the light cone from a 1.4 NA 40x oil objective (whether iris is closed or not) and a 40x long working distance dry objective are different. The depth of field varies with numerical aperture, so a 40x 1.4 oil immersion lens, should inherently have a shallower depth of focus. Therefore, it makes no intuitive sense to me that you would #1-increase the depth of focus, and #2- get the same results with a 40x oil 1.4 in which you stop down the iris to . 5NA, as a .5NA LWD dry lens. The excitation spot profile of the 40x oil objective is not going to enlarge as you stop down the iris with the same input light path. So I am trying to understand how the focal depth/resolution/xyz profile of the excitation/emission etc. is for fluorescence in comparing those two situations. Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
On Jun 15, 2007, at 9:19 AM, keith.morris-at-ucl.ac.uk wrote:
==============================Original Headers============================== 48, 25 -- From keith.morris-at-ucl.ac.uk Sat Jun 16 08:19:51 2007 48, 25 -- Received: from smtp3.global.net.uk (smtp3.global.net.uk [80.189.92.91]) 48, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5GDJoXt030808 48, 25 -- for {microscopy-at-microscopy.com} ; Sat, 16 Jun 2007 08:19:51 -0500 48, 25 -- Received: from 9.211.125.91.gr7.adsl.brightview.com ([91.125.211.9] helo=loungepc) 48, 25 -- by smtp3.global.net.uk with esmtp (Exim 4.66 (FreeBSD)) 48, 25 -- (envelope-from {keith.morris-at-ucl.ac.uk} ) 48, 25 -- id 1HzYBx-0009QA-GU 48, 25 -- for microscopy-at-microscopy.com; Sat, 16 Jun 2007 14:19:49 +0100 48, 25 -- From: "Keith Morris" {keith.morris-at-ucl.ac.uk} 48, 25 -- To: {microscopy-at-microscopy.com} 48, 25 -- References: {200706151424.l5FEOk8D025606-at-ns.microscopy.com} 48, 25 -- Subject: RE: [Microscopy] objective NA 48, 25 -- Date: Mon, 18 Jun 2007 14:19:52 +0100 48, 25 -- Message-ID: {000901c7b1ab$5b21e360$0501a8c0-at-loungepc} 48, 25 -- MIME-Version: 1.0 48, 25 -- Content-Type: text/plain; 48, 25 -- charset="iso-8859-1" 48, 25 -- X-Mailer: Microsoft Office Outlook 11 48, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 48, 25 -- Thread-Index: AcevWPLeAhOLNmY0SK6xlsnfsVMErQCSN70g 48, 25 -- In-Reply-To: {200706151424.l5FEOk8D025606-at-ns.microscopy.com} 48, 25 -- Authenticated-Sender: 48, 25 -- Content-Transfer-Encoding: 8bit 48, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5GDJoXt030808 ==============================End of - Headers==============================
OK, I've been looking around for a group that talks/works on/etc. older microscopes. But I have not been successful in finding such a group.
I have just picked up an old Olympus PMG metallograph. It is an absolutely fascinating piece of optical equipment. I've never seen one like it. Anyway, as one of my (ever increasing number of) extra-curricula activities, I've decided I'd like to either restore this instrument, or alter it so that I can actually use it as a modern metallograph with digital imaging capabilities. The first option would be my first choice, but if I can't get the parts needed to do that, I'll shoot for the second option...
My question to all the extraordinary minds on this group, does anyone know of someone that works on old microscopes? Does anyone know of a society or a group that discusses them, has old technical information about them, things like that?
I apologize if this is way off topic for this group. Please respond directly to me as I doubt the responses would be of general interest. If anyone does wish for a list of what I find, I'll be happy to share it, just let me know.
dj
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Email: tjzhang-at-umd.edu Name: Tim Zhang
Organization: UMD
Title-Subject: [Filtered] " wanted
Question: Dear All,
I need whole set of box of HV /Filament current Generator of "ESEM E3" urgently. I would like to "trade in" with any part of "ESEM E3" in my lab such as sample stage,HV cable, mult-DC power supply and so on.
If you have the circuit diagram of HV/Filament current Generator it would be great helpful.
We are looking for a postdoctoral or senior (S)TEM expert to join our group. A background in Materials Sciences, Physics, Engineering or Bio/Life Sciences would all be suitable. Projects concern the high-resolution structure determination of various membrane proteins and of DNA binding proteins, by electron crystallography and single particle cryo-EM, as well as method development in electron microscopy hardware for (S)TEM and remote controlling. Instrumentation is shared between our and the neighboring laboratory of Holland Cheng, and includes two or three FEG and one LaB6 TEMs, featuring cryo (including Helium) capabilities, energy filtration, STEM, Cs aberration correction, and large CCDs. Long-term funding is available. Salary will be commensurate with experience. Davis, California, is a family-friendly University town, located between the San Francisco / Berkeley bay area, and the Tahoe mountain lake and skiing area.
For further info, please contact me by email or phone, or at the upcoming 3DEM GRC.
Henning.
Henning Stahlberg, PhD Associate Professor, Molecular & Cellular Biology, Briggs Hall 5, University of California at Davis, 1 Shields Ave., Davis, CA 95616, USA Tel: +1-530-752 8282 (office), +1-530-754 8285 (lab) Fax: +1-530-752 3085 mailto:HStahlberg-at-ucdavis.edu Skype:henningstahlberg http://stahlberglab.ucdavis.edu http://2dx.org _____________________________________________________________
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Once upon a time I had to perform TEM on pineapples of different stages of ripeness, and I had problems that someone said was because glutaraldehyde doesn't fix well at low pH. (Nevermind the more pressing problem was the crystal of Si in each cell, and all that pineapple juice...) Now I have clients who want to fix bacteria that are being cultured at a pH of about 3.0. Do any of you have any suggestions for a fixation protocol? Cryo is not an option at this time.
Mahalo, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 19 -- From tina-at-pbrc.hawaii.edu Mon Jun 18 23:40:53 2007 4, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5J4eqot027780 4, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 18 Jun 2007 23:40:53 -0500 4, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id l5J4ekEe011567 4, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 18 Jun 2007 18:40:48 -1000 (HST) 4, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id l5J4ekL6011564 4, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 18 Jun 2007 18:40:46 -1000 (HST) 4, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 4, 19 -- Date: Mon, 18 Jun 2007 18:40:45 -1000 (HST) 4, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 19 -- X-Sender: tina-at-halia 4, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 4, 19 -- Subject: Fixing low pH extremophiles 4, 19 -- Message-ID: {Pine.GSO.4.21.0706181835070.11482-100000-at-halia} 4, 19 -- MIME-Version: 1.0 4, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
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Title-Subject: [Filtered] colloidal gold conjugation of proteins
Question: I am interested to preprare a protein-gold (5nm) complex to follow internalization of the protein into the cell. I used the protocols described by Hayat (colloidal gold , Principles, Methods and Applications) and previous recommandations of M. Bendayan but I failed... The protein is a small protein of 10kDa with a pHi of 8,5 and is dissolved in water. In parallel, I performed a control BSA-colloidal gold complex and it is OK. I tried to work with a pH of the gold solution slighly basic to the isoelectric pH of the protein, pH=9. I also tried to work at a pH of 7,4 and I also failed. In both cases, I added the colloidal gold (pH=9 or pH=7.4) to the protein solution. As recommended by M. Bendayan, I did not use at this step any PEG or other protective agents. I then centriguged the mixture at 45,000 rpm at 4ƒC for 60 min. The sediment at the bottom is dark-red. I recovered the sediment in PBS containing 0.02% PEG 20000. When I checked with negative staining with TEM, I did not visualise any gold particles or very few!!!. Is it the molecular weight of the protein a problem? Is the pH of the gold solution not optimal? Is a protective agent has to be used to stabilize the mixture befor centrifugation? Thanks a lot
Does anyone have experience storing glutaraldehyde-fixed tissues for potential TEM in 0.1M cacodylate buffer, 5% sucrose and thiomersal? Tissues may be stored for up to 3 months.
Thanks in advance! Sandy Hancock
VMRCVM Virginia Tech
==============================Original Headers============================== 7, 28 -- From skperkin-at-vt.edu Tue Jun 19 09:50:00 2007 7, 28 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu [198.82.162.213]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5JEo0Do031131 7, 28 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jun 2007 09:50:00 -0500 7, 28 -- Received: from vivi.cc.vt.edu (IDENT:mirapoint-at-evil-vivi.cc.vt.edu [10.1.1.12]) 7, 28 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id l5JEj8GH010561 7, 28 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jun 2007 10:49:59 -0400 7, 28 -- Received: from blade08.cc.vt.edu (imp.cc.vt.edu [198.82.161.55]) 7, 28 -- by vivi.cc.vt.edu (MOS 3.8.2-GA) 7, 28 -- with ESMTP id HPT28709; 7, 28 -- Tue, 19 Jun 2007 10:49:59 -0400 (EDT) 7, 28 -- Received: from blade08.cc.vt.edu (blade08.cc.vt.edu [127.0.0.1]) 7, 28 -- by blade08.cc.vt.edu (8.13.1/8.13.1) with ESMTP id l5JEnxQ0029529 7, 28 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jun 2007 10:49:59 -0400 7, 28 -- Received: (from apache-at-localhost) 7, 28 -- by blade08.cc.vt.edu (8.13.1/8.13.1/Submit) id l5JEnwWc029528 7, 28 -- for microscopy-at-microscopy.com; Tue, 19 Jun 2007 10:49:58 -0400 7, 28 -- Received: from vet9225.vetmed.vt.edu (vet9225.vetmed.vt.edu [128.173.229.225]) 7, 28 -- by webmail.vt.edu (IMP) with HTTP; Tue, 19 Jun 2007 10:49:58 -0400 7, 28 -- Message-ID: {1182264598.4677ed16e6e3c-at-webmail.vt.edu} 7, 28 -- Date: Tue, 19 Jun 2007 10:49:58 -0400 7, 28 -- From: skperkin-at-vt.edu 7, 28 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 7, 28 -- Subject: tissue storage 7, 28 -- MIME-Version: 1.0 7, 28 -- Content-Type: text/plain; charset=ISO-8859-1 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 ==============================End of - Headers==============================
O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with LaB6 guns. Our biggest concerns come in routine usability, and I am hoping to hear any comments on any of the following questions we have.
We handle a wide range of samples (Biological thin sections, organic macro-molecules, geologic particles, geologic thin sections, crystals, thin films, nano-particles, organic and non-organic materials) with a wide range of techniques (BF, DF, Diffraction, EDS, EELS, including lattice fringe imaging, STEM and tomography secondarily). In balancing contrast, resolution, and beam damage we really expect to perform a majority of our imaging between 80KeV and 120KeV (or 150KeV). However, the option of higher voltages up to 200KeV, for higher resolution imaging is something we are considering. So we are looking at both 120 and 200 KeV scopes. (And yes, we will test as many samples on any scope as we can but a couple of days worth of playing with a handful of samples does not compare to years worth of experience.)
(1) It seems that most 200Kev scopes are set to 200Kev and left there - presumably because it eases alignments, voltage stability, etc. when pushing for ultimate resolution. Is this true? Yes, all the manufacturers list voltage ranges the scope can be operated at, but does anyone really use anything else? With the digitizing of the scope controls and storage of multiple alignment setting, alignment at different voltages (theoretically) should be fairly straight forward but is it really?
(2) Is 200KeV worth having if the majority of the work does not require 200KeV? Not having personally worked much at 200KeV, Does loss of contrast and beam damage at 200KeV limit usability when imaging other than atomic / lattice level?
(3) If the majority of the scope usage is 80 - 140 KeV is the cost of a 200KeV instrument worth it? In terms of initial cost, and maintenance. (Even if the only option tops off at 120KeV). We are all aware of instances of: "If you can at all afford it get, even if you won´t use it regularly, because . . ." is this one of those?
(4) A few years ago the list had a discussion of W vs. LaB6, vs. FEG/Schottky for SEM´s and the consensus basically was that LaB6 was not worth the cost for SEM. (Either FEG if affordable or tungsten with better bells & whistles). Is LaB6 worth it in terms of TEM?
(5) What more important issue have we neglected in our naivete?
(Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?)
Thank you for any info you're willing to share!
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 15, 23 -- From edelmare-at-muohio.edu Tue Jun 19 11:09:28 2007 15, 23 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 15, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5JG9S9P012178 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 11:09:28 -0500 15, 23 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 15, 23 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l5JG9Sda025021 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 12:09:28 -0400 15, 23 -- Received: from [192.168.1.23] ([134.53.14.105]) 15, 23 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l5JG9R7T014789 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 12:09:27 -0400 15, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 15, 23 -- To: microscopy-at-Microscopy.com 15, 23 -- Date: Tue, 19 Jun 2007 12:09:26 -0400 15, 23 -- MIME-Version: 1.0 15, 23 -- Subject: 200KeV TEM users Opinions, please 15, 23 -- Message-ID: {4677C776.26865.13BE4E36-at-edelmare.muohio.edu} 15, 23 -- Priority: normal 15, 23 -- X-mailer: Pegasus Mail for Windows (4.41) 15, 23 -- Content-type: text/plain; charset=ISO-8859-1 15, 23 -- Content-description: Mail message body 15, 23 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 15, 23 -- Content-Transfer-Encoding: 8bit 15, 23 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id l5JG9S9P012178 ==============================End of - Headers==============================
Dear Richard, The first misconception is that 200 kV causes MORE beam damage than 100 kV. It causes less. More beam goes through the sample without dumping its energy, therefore less heat is dumped into the sample and there is less beam damage. Usable viewing area and diffraction is much improved at 200 kV. I have a 200 kV W-filament TEM. I use it at mainly 100 kV and 200 kV: 100 kV for light-element samples to get the contrast and 200 kV for better resolution and better penetration, therefore larger viewing area in metal samples. I have also looked at thick, unstained sections in botanical samples (0.5 micron, 0.75 micron and 1.0 micron thick) at 200 kV. If I were to replace this very old TEM, it would be with a 200 kV or 300 kV LaB6. The extra brightness is worth the small extra cost, the FEG in TEM is very expensive. I would get the STEM option so I could do EDS and EDS mapping. My TEM has five steps of acceleration voltage: 75, 100, 125, 150, 175 and 200 kV. All can be independently aligned and the alignment stored. Then, changing kV is simply pushing the button. In the new scopes, you can store the alignment on a CD or hard drive, so you can recover if one of your users screws things up horribly. I envy you the chance, I cannot get the people here to go seriously after a new TEM. Good luck. Regards,
-----Original Message----- X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu] Sent: June 19, 2007 9:18 AM To: maryflet-at-interchange.ubc.ca
200Kev scope users:
O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with LaB6 guns. Our biggest concerns come in routine usability, and I am hoping to hear any comments on any of the following questions we have.
We handle a wide range of samples (Biological thin sections, organic macro-molecules, geologic particles, geologic thin sections, crystals, thin films, nano-particles, organic and non-organic materials) with a wide range of techniques (BF, DF, Diffraction, EDS, EELS, including lattice fringe imaging, STEM and tomography secondarily). In balancing contrast, resolution, and beam damage we really expect to perform a majority of our imaging between 80KeV and 120KeV (or 150KeV). However, the option of higher voltages up to 200KeV, for higher resolution imaging is something we are considering. So we are looking at both 120 and 200 KeV scopes. (And yes, we will test as many samples on any scope as we can but a couple of days worth of playing with a handful of samples does not compare to years worth of experience.)
(1) It seems that most 200Kev scopes are set to 200Kev and left there - presumably because it eases alignments, voltage stability, etc. when pushing for ultimate resolution. Is this true? Yes, all the manufacturers list voltage ranges the scope can be operated at, but does anyone really use anything else? With the digitizing of the scope controls and storage of multiple alignment setting, alignment at different voltages (theoretically) should be fairly straight forward but is it really?
(2) Is 200KeV worth having if the majority of the work does not require 200KeV? Not having personally worked much at 200KeV, Does loss of contrast and beam damage at 200KeV limit usability when imaging other than atomic / lattice level?
(3) If the majority of the scope usage is 80 - 140 KeV is the cost of a 200KeV instrument worth it? In terms of initial cost, and maintenance. (Even if the only option tops off at 120KeV). We are all aware of instances of: "If you can at all afford it get, even if you won´t use it regularly, because . . ." is this one of those?
(4) A few years ago the list had a discussion of W vs. LaB6, vs. FEG/Schottky for SEM´s and the consensus basically was that LaB6 was not worth the cost for SEM. (Either FEG if affordable or tungsten with better bells & whistles). Is LaB6 worth it in terms of TEM?
(5) What more important issue have we neglected in our naivete?
(Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?)
Thank you for any info you're willing to share!
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 15, 23 -- From edelmare-at-muohio.edu Tue Jun 19 11:09:28 2007 15, 23 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 15, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5JG9S9P012178 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 11:09:28 -0500 15, 23 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 15, 23 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l5JG9Sda025021 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 12:09:28 -0400 15, 23 -- Received: from [192.168.1.23] ([134.53.14.105]) 15, 23 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l5JG9R7T014789 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 12:09:27 -0400 15, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 15, 23 -- To: microscopy-at-Microscopy.com 15, 23 -- Date: Tue, 19 Jun 2007 12:09:26 -0400 15, 23 -- MIME-Version: 1.0 15, 23 -- Subject: 200KeV TEM users Opinions, please 15, 23 -- Message-ID: {4677C776.26865.13BE4E36-at-edelmare.muohio.edu} 15, 23 -- Priority: normal 15, 23 -- X-mailer: Pegasus Mail for Windows (4.41) 15, 23 -- Content-type: text/plain; charset=ISO-8859-1 15, 23 -- Content-description: Mail message body 15, 23 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 15, 23 -- Content-Transfer-Encoding: 8bit 15, 23 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id l5JG9S9P012178 ==============================End of - Headers==============================
==============================Original Headers============================== 25, 36 -- From maryflet-at-interchange.ubc.ca Tue Jun 19 11:49:29 2007 25, 36 -- Received: from mr6.mail-relay.ubc.ca (mr6.mail-relay.ubc.ca [137.82.45.11]) 25, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5JGnTOj024508 25, 36 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jun 2007 11:49:29 -0500 25, 36 -- Received: from mr6.mail-relay.ubc.ca (localhost [127.0.0.1]) 25, 36 -- by localhost (Postfix) with SMTP id 262B9157FE 25, 36 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jun 2007 09:49:29 -0700 (PDT) 25, 36 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 25, 36 -- by mr6.mail-relay.ubc.ca (Postfix) with ESMTP 25, 36 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jun 2007 09:49:25 -0700 (PDT) 25, 36 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 25, 36 -- by smtp.interchange.ubc.ca 25, 36 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 25, 36 -- with ESMTPS id {0JJW003JG6QDI4-at-smtp.interchange.ubc.ca} for 25, 36 -- microscopy-at-microscopy.com; Tue, 19 Jun 2007 09:49:25 -0700 (PDT) 25, 36 -- Date: Tue, 19 Jun 2007 09:47:51 -0700 25, 36 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 25, 36 -- Subject: RE: [Microscopy] 200KeV TEM users Opinions, please 25, 36 -- In-reply-to: {200706191618.l5JGIPUM023658-at-ns.microscopy.com} 25, 36 -- To: edelmare-at-muohio.edu 25, 36 -- Cc: microscopy-at-microscopy.com 25, 36 -- Reply-to: maryflet-at-interchange.ubc.ca 25, 36 -- Message-id: {0JJW003JH6QDI4-at-smtp.interchange.ubc.ca} 25, 36 -- Organization: Materials Eng. 25, 36 -- MIME-version: 1.0 25, 36 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 25, 36 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 25, 36 -- Content-type: text/plain; charset=iso-8859-1 25, 36 -- Thread-index: AceyjXgxZdgNDrLwRyeE0Nzu8fdr+AAAjjCw 25, 36 -- X-UBC-Scanned: Sophos PureMessage 5.3.1.294258, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.6.19.93253 25, 36 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 25, 36 -- X-PerlMx-Spam: Probability=7%, Report=__C230066_P5 0, __CP_NAME_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __USER_AGENT_MS_GENERIC 0 25, 36 -- X-Spam-Level: 25, 36 -- X-Spam-Flag: No 25, 36 -- Content-Transfer-Encoding: 8bit 25, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5JGnTOj024508 ==============================End of - Headers==============================
In an universal applications environment the only good reason to buy 120kV vs 200kV TEM is lack of money.
=================================== Krassimir N. Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
On Jun 19, 2007, at 9:12 AM, edelmare-at-muohio.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } 200Kev scope users: } } O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with } LaB6 guns. Our biggest concerns come in routine usability, and I am } hoping to hear any comments on any of the following questions we } have. } } We handle a wide range of samples (Biological thin sections, organic } macro-molecules, geologic particles, geologic thin sections, } crystals, thin films, nano-particles, organic and non-organic } materials) with a wide range of techniques (BF, DF, Diffraction, EDS, } EELS, including lattice fringe imaging, STEM and tomography } secondarily). In balancing contrast, resolution, and beam damage we } really expect to perform a majority of our imaging between 80KeV and } 120KeV (or 150KeV). However, the option of higher voltages up to } 200KeV, for higher resolution imaging is something we are } considering. So we are looking at both 120 and 200 KeV scopes. (And } yes, we will test as many samples on any scope as we can but a couple } of days worth of playing with a handful of samples does not compare } to years worth of experience.) } } (1) It seems that most 200Kev scopes are set to 200Kev and left } there - presumably because it eases alignments, voltage stability, } etc. when pushing for ultimate resolution. Is this true? Yes, all } the manufacturers list voltage ranges the scope can be operated at, } but does anyone really use anything else? With the digitizing of the } scope controls and storage of multiple alignment setting, alignment } at different voltages (theoretically) should be fairly straight } forward but is it really? } } (2) Is 200KeV worth having if the majority of the work does not } require 200KeV? Not having personally worked much at 200KeV, Does } loss of contrast and beam damage at 200KeV limit usability when } imaging other than atomic / lattice level? } } (3) If the majority of the scope usage is 80 - 140 KeV is the cost } of a 200KeV instrument worth it? In terms of initial cost, and } maintenance. (Even if the only option tops off at 120KeV). We are } all aware of instances of: "If you can at all afford it get, even if } you won´t use it regularly, because . . ." is this one of those? } } (4) A few years ago the list had a discussion of W vs. LaB6, vs. } FEG/Schottky for SEM´s and the consensus basically was that LaB6 was } not worth the cost for SEM. (Either FEG if affordable or tungsten } with better bells & whistles). Is LaB6 worth it in terms of TEM? } } (5) What more important issue have we neglected in our naivete? } } (Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?) } } Thank you for any info you're willing to share! } } } } Richard E. Edelmann, Ph.D. } EXPO Editor, Microscopy and Microanalysis Supplement } Electron Microscopy Facility Director } 364 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } http://www.emf.muohio.edu } } } } ==============================Original } Headers============================== } 15, 23 -- From edelmare-at-muohio.edu Tue Jun 19 11:09:28 2007 } 15, 23 -- Received: from mulnx11.mcs.muohio.edu } (mulnx11.mcs.muohio.edu [134.53.6.66]) } 15, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l5JG9S9P012178 } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 } 11:09:28 -0500 } 15, 23 -- Received: from mulnx23.mcs.muohio.edu } (mulnx23.mcs.muohio.edu [134.53.6.10]) } 15, 23 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } with ESMTP id l5JG9Sda025021 } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 } 12:09:28 -0400 } 15, 23 -- Received: from [192.168.1.23] ([134.53.14.105]) } 15, 23 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } with ESMTP id l5JG9R7T014789 } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 } 12:09:27 -0400 } 15, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} } 15, 23 -- To: microscopy-at-Microscopy.com } 15, 23 -- Date: Tue, 19 Jun 2007 12:09:26 -0400 } 15, 23 -- MIME-Version: 1.0 } 15, 23 -- Subject: 200KeV TEM users Opinions, please } 15, 23 -- Message-ID: {4677C776.26865.13BE4E36-at-edelmare.muohio.edu} } 15, 23 -- Priority: normal } 15, 23 -- X-mailer: Pegasus Mail for Windows (4.41) } 15, 23 -- Content-type: text/plain; charset=ISO-8859-1 } 15, 23 -- Content-description: Mail message body } 15, 23 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 } 15, 23 -- Content-Transfer-Encoding: 8bit } 15, 23 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by } ns.microscopy.com id l5JG9S9P012178 } ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 22 -- From bozhilov-at-ucr.edu Tue Jun 19 12:02:16 2007 8, 22 -- Received: from sentinel.ucr.edu (sentinel.ucr.edu [138.23.226.228]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5JH2GW1003868 8, 22 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jun 2007 12:02:16 -0500 8, 22 -- Received: from [138.23.185.162] (igppb116g.ucr.edu [138.23.185.162]) 8, 22 -- by sentinel.ucr.edu (MOS 3.6.6-GR) 8, 22 -- with ESMTP id EOC72944 (AUTH via LOGINBEFORESMTP); 8, 22 -- Tue, 19 Jun 2007 10:02:12 -0700 (PDT) 8, 22 -- In-Reply-To: {200706191612.l5JGCCp6015586-at-ns.microscopy.com} 8, 22 -- References: {200706191612.l5JGCCp6015586-at-ns.microscopy.com} 8, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 8, 22 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 8, 22 -- Message-Id: {B215E817-DE93-4016-9941-AF6F8CE7CD9B-at-ucr.edu} 8, 22 -- Cc: microscopy-at-microscopy.com 8, 22 -- From: "K.N. Bozhilov" {bozhilov-at-ucr.edu} 8, 22 -- Subject: Re: [Microscopy] 200KeV TEM users Opinions, please 8, 22 -- Date: Tue, 19 Jun 2007 10:02:12 -0700 8, 22 -- To: edelmare-at-muohio.edu 8, 22 -- X-Mailer: Apple Mail (2.752.2) 8, 22 -- X-Junkmail-Whitelist: YES (by domain whitelist at sentinel.ucr.edu) 8, 22 -- Content-Transfer-Encoding: 8bit 8, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5JH2GW1003868 ==============================End of - Headers==============================
If you are in the Northeast, get in touch with either John Oren (VT Optics - 802-425-2040) or Dennis O'Leary (MicroOptical Methods 518-482-8200 or rdenol-at-hotmail.com). Alternatively, Don Thomson (see CC above) in the UK does a lot of this sort of work. Also, the Queckett Society in the UK does a lot with old microscopes... Don can answer more questions about them.
Have FUN!!!
Best regards, Barbara Foster, President
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At 09:36 AM 6/19/2007, you wrote:
==============================Original Headers============================== 12, 16 -- From bfoster-at-mme1.com Tue Jun 19 14:35:19 2007 12, 16 -- Received: from smtp104.sbc.mail.mud.yahoo.com (smtp104.sbc.mail.mud.yahoo.com [68.142.198.203]) 12, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5JJZJrs019479 12, 16 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jun 2007 14:35:19 -0500 12, 16 -- Message-Id: {200706191935.l5JJZJrs019479-at-ns.microscopy.com} 12, 16 -- Received: (qmail 4705 invoked from network); 19 Jun 2007 19:35:18 -0000 12, 16 -- Received: from unknown (HELO barbsd505.mme1.com) (bfostermme-at-sbcglobal.net-at-68.94.58.35 with login) 12, 16 -- by smtp104.sbc.mail.mud.yahoo.com with SMTP; 19 Jun 2007 19:35:18 -0000 12, 16 -- X-YMail-OSG: HceHiOwVM1nYzr3zen5BNqtr5vA2qcy8H8m8Hi85JuclIVO7YINO_9THgdA.MuBy3SUxxqRwZ2lLU9fDV96D_IjcmkPOeAl7rWeqSNwVblIvys8YUsg- 12, 16 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 12, 16 -- Date: Tue, 19 Jun 2007 14:34:13 -0500 12, 16 -- To: microscopy-at-microscopy.com 12, 16 -- From: Barbara Foster {bfoster-at-mme1.com} 12, 16 -- Subject: Re: Unusual request 12, 16 -- Mime-Version: 1.0 12, 16 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
} -----Original Message----- } X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu] } Sent: June 19, 2007 9:18 AM } To: maryflet-at-interchange.ubc.ca } Subject: [Microscopy] 200KeV TEM users Opinions, please } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
I well remember my days as a TEM demonstrator trying to persuade users, often unsuccessfuly, that going to higher kV really did reduce damage .......
However, while for many samples, higher kV does result in reduced thermal damage, for some samples knock-on damage begins at surprisingly low thresholds and this does increase as the kV increases. A particularly topical example is carbon nanotubes, where there is evidence that knock-on damage begins at ~80 kV ...
You also need to remember that with digital CCD camera systems, image contrast is much less dependent on kV, so with a good digital camera, you can get both improved resolution and good image contrast by operating at 200 kV.
LaB6 is definitely worth using on a TEM. Unlike SEMs, there is generally no additional cost apart from the filaments themselves, since the gun vacuum levels on modern instruments should be more than sufficient for LaB6. At higher magnifications, the combination of greater brightness and smaller spot sizes usually means that image are brighter. And filament lifetimes should be significantly longer. LaB6 filaments also give somewhat better beam coherence, which in practical terms results in better contrast in high resolution images.
My feeling is that really the only reason to go for 100/120 KV is that the budget isn't availabe for 200 kV.
And while you can always turn the kV down on a 200 kV instrument, you can't turn it up on a 100/120 kV instrument!
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==============================Original Headers============================== 13, 18 -- From larry-at-celtic.freewire.co.uk Tue Jun 19 15:51:10 2007 13, 18 -- Received: from get.freewire.net (get.freewire.net [86.54.106.202] (may be forged)) 13, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5JKp97a000701 13, 18 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 19 Jun 2007 15:51:09 -0500 13, 18 -- Received: from [217.154.251.251] (th6dc-217-154-251-251.dial.mistral.co.uk [217.154.251.251] (may be forged)) 13, 18 -- by get.freewire.net (8.13.6/8.11.6) with ESMTP id l5JKp3Kg011367 13, 18 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 19 Jun 2007 21:51:05 +0100 13, 18 -- Mime-Version: 1.0 13, 18 -- Message-Id: {p06210200c29ded7ce27f-at-[217.154.251.236]} 13, 18 -- In-Reply-To: {200706191655.l5JGt6aY001772-at-ns.microscopy.com} 13, 18 -- References: {200706191655.l5JGt6aY001772-at-ns.microscopy.com} 13, 18 -- Date: Tue, 19 Jun 2007 21:50:02 +0100 13, 18 -- To: Microscopy-at-MSA.Microscopy.Com 13, 18 -- From: Larry Stoter {larry-at-celtic.freewire.co.uk} 13, 18 -- Subject: [Microscopy] RE: 200KeV TEM users Opinions, please 13, 18 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 13, 18 -- Content-Transfer-Encoding: 8bit 13, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5JKp97a000701 ==============================End of - Headers==============================
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Title-Subject: [Filtered] colloidal gold conjugation of proteins
Question: I am interested to preprare a protein-gold (5nm) complex to follow internalization of the protein into the cell. I used the protocols described by Hayat (colloidal gold , Principles, Methods and Applications) and previous recommandations of M. Bendayan but I failed... The protein is a small protein of 10kDa with a pHi of 8,5 and is dissolved in water. In parallel, I performed a control BSA-colloidal gold complex and it is OK. I tried to work with a pH of the gold solution slighly basic to the isoelectric pH of the protein, pH=9. I also tried to work at a pH of 7,4 and I also failed. In both cases, I added the colloidal gold (pH=9 or pH=7.4) to the protein solution. As recommended by M. Bendayan, I did not use at this step any PEG or other protective agents. I then centriguged the mixture at 45,000 rpm at 4ƒC for 60 min. The sediment at the bottom is dark-red. I recovered the sediment in PBS containing 0.02% PEG 20000. When I checked with negative staining with TEM, I did not visualise any gold particles or very few!!!. Is it the molecular weight of the protein a problem? Is the pH of the gold solution not optimal? Is a protective agent has to be used to stabilize the mixture befor centrifugation? Thanks a lot
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Email: allanwojtasp-at-agr.gc.ca Name: Paula Allan-Wojtas
Organization: Agriculture and Agri-Food Canada
Title-Subject: [Filtered] Can a tissue fixation protocol be patented?
Question: I have just heard that a company has just patented a tissue fixation protocol. Is this possible? It doesn't sound correct to me, since a number of papers have been published in the scientific literature over the past 10 years which have used this particular protocol already. Can this really happen? What are the repercussions if this is true?
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Organization: Southern Connecticut State University
Title-Subject: [Filtered] RE: Segmentation of particles in Gatan Digital Micrograph Software
Question: Hello,
I am an undergraduate student at Southern Connecticut State University involved in research. I was wondering if there was any way of segmenting clustered particles in Gatan. I know that you can apply a watershed algorithm to binary images in ImageJ, but I am looking for the equivalent in Gatan. I am also open to any scripts that I can use to apply segmentation to the images within Gatan.
Then you have no problem running any KeV at any time in your TEM(s)?
On 19 Jun 2007 at 10:02, K.N. Bozhilov wrote:
} In an universal applications environment the only good reason to buy } 120kV vs 200kV TEM is lack of money. } } =================================== } Krassimir N. Bozhilov } Central Facility for Advanced Microscopy and Microanalysis } University of California } Riverside, CA 92521 } } tel. 951 827 2998 } fax 951 827 2489 } =================================== } } } On Jun 19, 2007, at 9:12 AM, edelmare-at-muohio.edu wrote: } } } } } } } } } ---------------------------------------------------------------------- } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------- } } ------ } } } } 200Kev scope users: } } } } O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with } } LaB6 guns. Our biggest concerns come in routine usability, and I am } } hoping to hear any comments on any of the following questions we } } have. } } } } We handle a wide range of samples (Biological thin sections, organic } } macro-molecules, geologic particles, geologic thin sections, } } crystals, thin films, nano-particles, organic and non-organic } } materials) with a wide range of techniques (BF, DF, Diffraction, EDS, } } EELS, including lattice fringe imaging, STEM and tomography } } secondarily). In balancing contrast, resolution, and beam damage we } } really expect to perform a majority of our imaging between 80KeV and } } 120KeV (or 150KeV). However, the option of higher voltages up to } } 200KeV, for higher resolution imaging is something we are } } considering. So we are looking at both 120 and 200 KeV scopes. (And } } yes, we will test as many samples on any scope as we can but a couple } } of days worth of playing with a handful of samples does not compare } } to years worth of experience.) } } } } (1) It seems that most 200Kev scopes are set to 200Kev and left } } there - presumably because it eases alignments, voltage stability, } } etc. when pushing for ultimate resolution. Is this true? Yes, all } } the manufacturers list voltage ranges the scope can be operated at, } } but does anyone really use anything else? With the digitizing of the } } scope controls and storage of multiple alignment setting, alignment } } at different voltages (theoretically) should be fairly straight } } forward but is it really? } } } } (2) Is 200KeV worth having if the majority of the work does not } } require 200KeV? Not having personally worked much at 200KeV, Does } } loss of contrast and beam damage at 200KeV limit usability when } } imaging other than atomic / lattice level? } } } } (3) If the majority of the scope usage is 80 - 140 KeV is the cost } } of a 200KeV instrument worth it? In terms of initial cost, and } } maintenance. (Even if the only option tops off at 120KeV). We are } } all aware of instances of: "If you can at all afford it get, even if } } you won´t use it regularly, because . . ." is this one of those? } } } } (4) A few years ago the list had a discussion of W vs. LaB6, vs. } } FEG/Schottky for SEM´s and the consensus basically was that LaB6 was } } not worth the cost for SEM. (Either FEG if affordable or tungsten } } with better bells & whistles). Is LaB6 worth it in terms of TEM? } } } } (5) What more important issue have we neglected in our naivete? } } } } (Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?) } } } } Thank you for any info you're willing to share! } } } } } } } } Richard E. Edelmann, Ph.D. } } EXPO Editor, Microscopy and Microanalysis Supplement } } Electron Microscopy Facility Director } } 364 Pearson Hall } } Miami University, Oxford, OH 45056 } } Ph: 513.529.5712 Fax: 513.529.4243 } } E-mail: edelmare-at-muohio.edu } } http://www.emf.muohio.edu } } } } } } } } ==============================Original } } Headers============================== } } 15, 23 -- From edelmare-at-muohio.edu Tue Jun 19 11:09:28 2007 } } 15, 23 -- Received: from mulnx11.mcs.muohio.edu } } (mulnx11.mcs.muohio.edu [134.53.6.66]) } } 15, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id l5JG9S9P012178 } } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 } } 11:09:28 -0500 } } 15, 23 -- Received: from mulnx23.mcs.muohio.edu } } (mulnx23.mcs.muohio.edu [134.53.6.10]) } } 15, 23 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } } with ESMTP id l5JG9Sda025021 } } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 } } 12:09:28 -0400 } } 15, 23 -- Received: from [192.168.1.23] ([134.53.14.105]) } } 15, 23 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } } with ESMTP id l5JG9R7T014789 } } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 } } 12:09:27 -0400 } } 15, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} } } 15, 23 -- To: microscopy-at-Microscopy.com } } 15, 23 -- Date: Tue, 19 Jun 2007 12:09:26 -0400 } } 15, 23 -- MIME-Version: 1.0 } } 15, 23 -- Subject: 200KeV TEM users Opinions, please } } 15, 23 -- Message-ID: {4677C776.26865.13BE4E36-at-edelmare.muohio.edu} } } 15, 23 -- Priority: normal } } 15, 23 -- X-mailer: Pegasus Mail for Windows (4.41) } } 15, 23 -- Content-type: text/plain; charset=ISO-8859-1 } } 15, 23 -- Content-description: Mail message body } } 15, 23 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 } } 15, 23 -- Content-Transfer-Encoding: 8bit } } 15, 23 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by } } ns.microscopy.com id l5JG9S9P012178 } } ==============================End of - } } Headers============================== }
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
==============================Original Headers============================== 10, 25 -- From edelmare-at-Muohio.edu Wed Jun 20 07:23:44 2007 10, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5KCNi18032133 10, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 07:23:44 -0500 10, 25 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 10, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l5KCKQud008353; 10, 25 -- Wed, 20 Jun 2007 08:20:26 -0400 10, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 10, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l5KCKPCw001786; 10, 25 -- Wed, 20 Jun 2007 08:20:25 -0400 10, 25 -- From: "Richard E. Edelmann" {edelmare-at-Muohio.edu} 10, 25 -- To: "K.N. Bozhilov" {bozhilov-at-ucr.edu} , microscopy-at-microscopy.com 10, 25 -- Date: Wed, 20 Jun 2007 08:20:25 -0400 10, 25 -- MIME-Version: 1.0 10, 25 -- Subject: Re: [Microscopy] 200KeV TEM users Opinions, please 10, 25 -- Message-ID: {4678E349.30182.1812C59F-at-edelmare.muohio.edu} 10, 25 -- Priority: normal 10, 25 -- In-reply-to: {B215E817-DE93-4016-9941-AF6F8CE7CD9B-at-ucr.edu} 10, 25 -- References: {200706191612.l5JGCCp6015586-at-ns.microscopy.com} , {B215E817-DE93-4016-9941-AF6F8CE7CD9B-at-ucr.edu} 10, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 10, 25 -- Content-type: text/plain; charset=ISO-8859-1 10, 25 -- Content-description: Mail message body 10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 10, 25 -- Content-Transfer-Encoding: 8bit 10, 25 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id l5KCNi18032133 ==============================End of - Headers==============================
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Email: rdsouza78-at-hotmail.com Name: Ryan D'Souza
Organization: National Institute For Research In Reproductive Health
Question: I would like to perform an immunohistochemical analysis on cryosections ( with DAB) and then process them for TEM. I have come across several articles that use the INVERTED GELATIN CAPSULE METHOD. i would appreciate if some one could give me an insight/ detailed protocol on how the technique is done.
If I remember well this topic was discussed some time ago and it was said that was ok in cacodylate buffer as long as this is not stored at 37°C ;-) My 5 cent: perhaps some calcium could help too.
regards,
Stephane
--- skperkin-at-vt.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } Hello- } } Does anyone have experience storing } glutaraldehyde-fixed tissues for potential } TEM in 0.1M cacodylate buffer, 5% sucrose and } thiomersal? Tissues may be } stored for up to 3 months. } } Thanks in advance! } Sandy Hancock } } } VMRCVM } Virginia Tech } } ==============================Original } Headers============================== } 7, 28 -- From skperkin-at-vt.edu Tue Jun 19 09:50:00 } 2007 } 7, 28 -- Received: from lennier.cc.vt.edu } (lennier.cc.vt.edu [198.82.162.213]) } 7, 28 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l5JEo0Do031131 } 7, 28 -- for {microscopy-at-microscopy.com} ; Tue, 19 } Jun 2007 09:50:00 -0500 } 7, 28 -- Received: from vivi.cc.vt.edu } (IDENT:mirapoint-at-evil-vivi.cc.vt.edu [10.1.1.12]) } 7, 28 -- by lennier.cc.vt.edu } (8.12.11.20060308/8.12.11) with ESMTP id } l5JEj8GH010561 } 7, 28 -- for {microscopy-at-microscopy.com} ; Tue, 19 } Jun 2007 10:49:59 -0400 } 7, 28 -- Received: from blade08.cc.vt.edu } (imp.cc.vt.edu [198.82.161.55]) } 7, 28 -- by vivi.cc.vt.edu (MOS 3.8.2-GA) } 7, 28 -- with ESMTP id HPT28709; } 7, 28 -- Tue, 19 Jun 2007 10:49:59 -0400 (EDT) } 7, 28 -- Received: from blade08.cc.vt.edu } (blade08.cc.vt.edu [127.0.0.1]) } 7, 28 -- by blade08.cc.vt.edu (8.13.1/8.13.1) with } ESMTP id l5JEnxQ0029529 } 7, 28 -- for {microscopy-at-microscopy.com} ; Tue, 19 } Jun 2007 10:49:59 -0400 } 7, 28 -- Received: (from apache-at-localhost) } 7, 28 -- by blade08.cc.vt.edu } (8.13.1/8.13.1/Submit) id l5JEnwWc029528 } 7, 28 -- for microscopy-at-microscopy.com; Tue, 19 Jun } 2007 10:49:58 -0400 } 7, 28 -- Received: from vet9225.vetmed.vt.edu } (vet9225.vetmed.vt.edu [128.173.229.225]) } 7, 28 -- by webmail.vt.edu (IMP) with HTTP; Tue, 19 } Jun 2007 10:49:58 -0400 } 7, 28 -- Message-ID: } {1182264598.4677ed16e6e3c-at-webmail.vt.edu} } 7, 28 -- Date: Tue, 19 Jun 2007 10:49:58 -0400 } 7, 28 -- From: skperkin-at-vt.edu } 7, 28 -- To: Microscopy Listserver } {microscopy-at-microscopy.com} } 7, 28 -- Subject: tissue storage } 7, 28 -- MIME-Version: 1.0 } 7, 28 -- Content-Type: text/plain; } charset=ISO-8859-1 } 7, 28 -- Content-Transfer-Encoding: 8bit } 7, 28 -- User-Agent: Internet Messaging Program } (IMP) 3.2.8 } ==============================End of - } Headers============================== }
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==============================Original Headers============================== 9, 21 -- From nizets2-at-yahoo.com Wed Jun 20 09:15:21 2007 9, 21 -- Received: from web37414.mail.mud.yahoo.com (web37414.mail.mud.yahoo.com [209.191.91.146]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5KEFKxb025587 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 09:15:20 -0500 9, 21 -- Received: (qmail 9615 invoked by uid 60001); 20 Jun 2007 14:15:20 -0000 9, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 21 -- s=s1024; d=yahoo.com; 9, 21 -- h=X-YMail-OSG:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 9, 21 -- b=CQAjdMsEnOe8+wm1uLktGKBsb45OjUYAra7nvcw2oh9IkcuTro3P/NWrlQQuvkdueoadCevK9CcjhGFHykY6kROgaSsH2sYcrHI4SJ0lcDtFXRuzWkPG5q/4A289I/wwIj8PxR8Kn4k1mFLFYNVdOpetitAQsNDMNJJFEe/5C+8=; 9, 21 -- X-YMail-OSG: 50kAfe4VM1mdrUTi3gjkHvwc45fRr1B3TAcWXH40TXTvA9vL0K1MO4rt.IkusCWd49sQb_UM2S1XLpAEmeB5YqoY2EHOhxNSj0UtrtmS01Qktm02kj4wDbNxK3YMHNkNzYQdcb.qhdBzHh8dhWU9xkeQOXS.QEs7X3TXQe3gSFITed4lO4gbf9Q- 9, 21 -- Received: from [80.122.101.102] by web37414.mail.mud.yahoo.com via HTTP; Wed, 20 Jun 2007 07:15:20 PDT 9, 21 -- Date: Wed, 20 Jun 2007 07:15:20 -0700 (PDT) 9, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 9, 21 -- Subject: Re: [Microscopy] tissue storage 9, 21 -- To: skperkin-at-vt.edu 9, 21 -- Cc: microscopy-at-microscopy.com 9, 21 -- In-Reply-To: {200706191457.l5JEvJGv006399-at-ns.microscopy.com} 9, 21 -- MIME-Version: 1.0 9, 21 -- Content-Type: text/plain; charset=iso-8859-1 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- Message-ID: {503910.2599.qm-at-web37414.mail.mud.yahoo.com} ==============================End of - Headers==============================
I will join the opinion of previous posters of the list. A LaB6 at 200keV just offers more flexibility for no disadvantages except the cost (I don't know the difference with W gun). With my TEM I can even work at 1 keV if I want to(which I doesn't ;-)) And I can store all the settings I want at any keV or any magnification I choose (you can still ask the company about these important "details"). I would add that if you plan to work on nanoparticles you will probably appreciate the additional comfort of a LaB6 working at 200keV at high mag.
Best regards,
Stephane
--- edelmare-at-muohio.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } 200Kev scope users: } } O.k., we are trying to evaluate 120Kev TEM´s vs } 200Kev TEM´s with } LaB6 guns. Our biggest concerns come in routine } usability, and I am } hoping to hear any comments on any of the following } questions we } have. } } We handle a wide range of samples (Biological thin } sections, organic } macro-molecules, geologic particles, geologic thin } sections, } crystals, thin films, nano-particles, organic and } non-organic } materials) with a wide range of techniques (BF, DF, } Diffraction, EDS, } EELS, including lattice fringe imaging, STEM and } tomography } secondarily). In balancing contrast, resolution, } and beam damage we } really expect to perform a majority of our imaging } between 80KeV and } 120KeV (or 150KeV). However, the option of higher } voltages up to } 200KeV, for higher resolution imaging is something } we are } considering. So we are looking at both 120 and 200 } KeV scopes. (And } yes, we will test as many samples on any scope as we } can but a couple } of days worth of playing with a handful of samples } does not compare } to years worth of experience.) } } (1) It seems that most 200Kev scopes are set to } 200Kev and left } there - presumably because it eases alignments, } voltage stability, } etc. when pushing for ultimate resolution. Is this } true? Yes, all } the manufacturers list voltage ranges the scope can } be operated at, } but does anyone really use anything else? With the } digitizing of the } scope controls and storage of multiple alignment } setting, alignment } at different voltages (theoretically) should be } fairly straight } forward but is it really? } } (2) Is 200KeV worth having if the majority of the } work does not } require 200KeV? Not having personally worked much } at 200KeV, Does } loss of contrast and beam damage at 200KeV limit } usability when } imaging other than atomic / lattice level? } } (3) If the majority of the scope usage is 80 - 140 } KeV is the cost } of a 200KeV instrument worth it? In terms of } initial cost, and } maintenance. (Even if the only option tops off at } 120KeV). We are } all aware of instances of: "If you can at all } afford it get, even if } you won´t use it regularly, because . . ." is this } one of those? } } (4) A few years ago the list had a discussion of W } vs. LaB6, vs. } FEG/Schottky for SEM´s and the consensus basically } was that LaB6 was } not worth the cost for SEM. (Either FEG if } affordable or tungsten } with better bells & whistles). Is LaB6 worth it in } terms of TEM? } } (5) What more important issue have we neglected in } our naivete? } } (Boy, wouldn´t a 150KeV Schottky-FEG be a nice } compromise?) } } Thank you for any info you're willing to share! } } } } Richard E. Edelmann, Ph.D. } EXPO Editor, Microscopy and Microanalysis Supplement } Electron Microscopy Facility Director } 364 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } http://www.emf.muohio.edu } } } } ==============================Original } Headers============================== } 15, 23 -- From edelmare-at-muohio.edu Tue Jun 19 } 11:09:28 2007 } 15, 23 -- Received: from mulnx11.mcs.muohio.edu } (mulnx11.mcs.muohio.edu [134.53.6.66]) } 15, 23 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l5JG9S9P012178 } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 } Jun 2007 11:09:28 -0500 } 15, 23 -- Received: from mulnx23.mcs.muohio.edu } (mulnx23.mcs.muohio.edu [134.53.6.10]) } 15, 23 -- by mulnx11.mcs.muohio.edu } (Switch-3.1.8/Switch-3.1.7) with ESMTP id } l5JG9Sda025021 } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 } Jun 2007 12:09:28 -0400 } 15, 23 -- Received: from [192.168.1.23] } ([134.53.14.105]) } 15, 23 -- by mulnx23.mcs.muohio.edu } (Switch-3.1.8/Switch-3.1.7) with ESMTP id } l5JG9R7T014789 } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 } Jun 2007 12:09:27 -0400 } 15, 23 -- From: "Richard E. Edelmann" } {edelmare-at-muohio.edu} } 15, 23 -- To: microscopy-at-Microscopy.com } 15, 23 -- Date: Tue, 19 Jun 2007 12:09:26 -0400 } 15, 23 -- MIME-Version: 1.0 } 15, 23 -- Subject: 200KeV TEM users Opinions, please } 15, 23 -- Message-ID: } {4677C776.26865.13BE4E36-at-edelmare.muohio.edu} } 15, 23 -- Priority: normal } 15, 23 -- X-mailer: Pegasus Mail for Windows (4.41) } 15, 23 -- Content-type: text/plain; } charset=ISO-8859-1 } 15, 23 -- Content-description: Mail message body } 15, 23 -- X-Scanned-By: MIMEDefang 2.57 on } 134.53.6.66 } 15, 23 -- Content-Transfer-Encoding: 8bit } 15, 23 -- X-MIME-Autoconverted: from } Quoted-printable to 8bit by ns.microscopy.com id } l5JG9S9P012178 } ==============================End of - } Headers============================== }
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==============================Original Headers============================== 17, 21 -- From nizets2-at-yahoo.com Wed Jun 20 09:40:32 2007 17, 21 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 17, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5KEeWtZ005398 17, 21 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 09:40:32 -0500 17, 21 -- Received: (qmail 73561 invoked by uid 60001); 20 Jun 2007 14:40:32 -0000 17, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 17, 21 -- s=s1024; d=yahoo.com; 17, 21 -- h=X-YMail-OSG:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 17, 21 -- b=toa6sH0fu+JRHWaENyLVTmy49zEtgDMGBcBud6CBenYQuuFWR6OMCxgeuW9dIvEAlgqpxVMVw0v2DdC9YXpSE7sum21azLyHn5h6Qf1O0HwewLsGUJZ7e6QEDXVCN5ngwtWgcpnUu1UGbhTAw3DJnEC4p4u+hlcJhPSgBjHRXh4=; 17, 21 -- X-YMail-OSG: ehVsXisVM1mLcu54dAwv7f9qqv3ry.EQaBwNUg5yg2hKeHHDEA_tqaCkFcEWdXuTrCY6aQI07zOTyABRQEUAsK2s5VgWpO65l2MYcKeO2a3B_ohPfHhoxC9wMcHL9wz5MMNLF.38epFb2U8DEgR8WDu.lDvHgFyVIcc1fj5CrfpjxoYttdSXswQ- 17, 21 -- Received: from [80.122.101.102] by web37405.mail.mud.yahoo.com via HTTP; Wed, 20 Jun 2007 07:40:32 PDT 17, 21 -- Date: Wed, 20 Jun 2007 07:40:32 -0700 (PDT) 17, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 17, 21 -- Subject: Re: [Microscopy] 200KeV TEM users Opinions, please 17, 21 -- To: edelmare-at-muohio.edu 17, 21 -- Cc: microscopy-at-microscopy.com 17, 21 -- In-Reply-To: {200706191616.l5JGGFkr019830-at-ns.microscopy.com} 17, 21 -- MIME-Version: 1.0 17, 21 -- Content-Type: text/plain; charset=iso-8859-1 17, 21 -- Content-Transfer-Encoding: 8bit 17, 21 -- Message-ID: {344453.40930.qm-at-web37405.mail.mud.yahoo.com} ==============================End of - Headers==============================
Well, this kind of thing has been done in the past - wasn't the tyramide amplification technique originally published by a research group, then patented by a company? I'm sure there are other instances; this one comes to mind because there were discussions of it at the time on one of the on-line groups - maybe this one?
Anyway - which fixation?
Tamara
On Tue, 19 Jun 2007 22:45:33 -0500 allanwojtasp-at-agr.gc.ca wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy } Listserver } using the WWW based Form at } http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a } Subscriber, so when replying } please copy both allanwojtasp-at-agr.gc.ca as well as } the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: allanwojtasp-at-agr.gc.ca } Name: Paula Allan-Wojtas } } Organization: Agriculture and Agri-Food Canada } } Title-Subject: [Filtered] Can a tissue fixation protocol } be patented? } } Question: I have just heard that a company has just } patented a tissue fixation protocol. Is this possible? It } doesn't sound correct to me, since a number of papers } have been published in the scientific literature over the } past 10 years which have used this particular protocol } already. Can this really happen? What are the } repercussions if this is true? } } } } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Tue Jun 19 22:43:45 } 2007 } 7, 11 -- Received: from [206.69.208.22] } (mac22.zaluzec.com [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id l5K3hjlF019946 } 7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jun } 2007 22:43:45 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: } {p06240802c29e52e1e4a8-at-[206.69.208.22]} } 7, 11 -- Date: Tue, 19 Jun 2007 22:43:43 -0500 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: allanwojtasp-at-agr.gc.ca (by way of } MicroscopyListserver) } 7, 11 -- Subject: viaWWW: Can a tissue fixation protocol } be patented? } 7, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers==============================
} Date: Wed, 20 Jun 2007 10:05:30 -0500 } From: Daryl Meyer {dameyer-at-wisc.edu} } Subject: Re: Fwd: [Microscopy] viaWWW: colloidal gold conjugation of proteins } To: Philip Oshel {oshel1pe-at-cmich.edu} } } Phil, I just subscribed, but it looks like it } may take a day to be able to access the list, so } I'll let you forward my response: } } I don't think the problem lies with the MW of } the protein or the pH of 9 for conjugation. } } To better address the question, I need a little } more info. First, was a concentration isotherm } performed at either pH to determine the amount } of protein necessary to stabilize a given volume } of cAu against electrolytic floculation (ie, } influx of saturated NaCl)? This by itself will } go a long way in telling whether or not stable } conjugates are present. If the conjugates remain } red in color above a certain protein } concentration following addition of salt, then } it would seem that the reason very few particles } are visualized by TEM is that either the } sedimented conjugates were too dilute following } resuspension, or they were not allowed to adhere } to the grids for a long enough period of time. } For labeling experiments, we generally resuspend } to one-tenth of the original volume of cAu, } giving a particle concentration of roughly 10^13 } per ml. We've found this to effectively minimize } the amount of time required to saturate all } binding sites on a cell surface. At this } concentration, } a grid should only need to sit on a drop of the } conjugate for 20 minutes or so at room temp for } there to be plenty of particles for imaging. } } To visualize the protein coat around the } particles by negative staining, the PEG has to } be omitted entirely in order to be sure that } whatever surrounds the particles is definitely } protein and not PEG. If the conjugates are not } stable in PBS without PEG, then resuspend in } water to do the negative staining. If PEG is } needed as a secondary stabilizer in PBS for the } labeling, I add it after conjugation, but before } centrifugation. But remember that it's important } to do the initial concentration isotherm in } order to be sure that protein is bound to the } particles, and that it's not simply the PEG } that's stabilizing them. } } I hope this is helpful. } } Daryl } } } } } Date: Tue, 19 Jun 2007 22:50:28 -0500 } } } } } To: oshel1pe-at-cmich.edu } } } } } From: lhermini-at-epoisses.inra.fr } } } } } Reply-to: lhermini-at-epoisses.inra.fr } } } } } } } ---------------------------------------------------------------------------- } } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society } } of America } } } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } } ---------------------------------------------------------------------------- } } } } } } } } } } This Question/Comment was submitted to the Microscopy Listserver } } } } } using the WWW based Form at } } } } } } } --------------------------------------------------------------------------- } } } } } Remember this posting is most likely not from } } } } a Subscriber, so when replying } } } } } please copy both lhermini-at-epoisses.inra.fr as } } } } } well as the MIcroscopy Listserver } } } } } } } --------------------------------------------------------------------------- } } } } } } } } } } Email: lhermini-at-epoisses.inra.fr } } } } } Name: jeannine Lherminier } } } } } } } } } } Organization: INRA } } } } } } } } } } Title-Subject: [Filtered] colloidal gold conjugation of proteins } } } } } } } } } } Question: I am interested to preprare a } } } } } protein-gold (5nm) complex to follow } } } } } internalization of the protein into the cell. I } } } } } used the protocols described by Hayat (colloidal } } } } } gold , Principles, Methods and Applications) and } } } } } previous recommandations of M. Bendayan but I } } } } } failed... The protein is a small protein of } } } } } 10kDa with a pHi of 8,5 and is dissolved in } } } } } water. In parallel, I performed a control } } } } } BSA-colloidal gold complex and it is OK. I tried } } } } } to work with a pH of the gold solution slighly } } } } } basic to the isoelectric pH of the protein, } } } } } pH=9. I also tried to work at a pH of 7,4 and I } } } } } also failed. In both cases, I added the } } } } } colloidal gold (pH=9 or pH=7.4) to the protein } } } } } solution. As recommended by M. Bendayan, I did } } } } } not use at this step any PEG or other protective } } } } } agents. I then centriguged the mixture at 45,000 } } } } } rpm at 4ÉC for 60 min. The sediment at the } } } } } bottom is dark-red. I recovered the sediment in } } } } } PBS containing 0.02% PEG 20000. When I checked } } } } } with negative staining with TE! } } } } } M, I did not visualise any gold particles or very few!!!. } } } } } Is it the molecular weight of the protein a } } } } } problem? Is the pH of the gold solution not } } } } } optimal? Is a protective agent has to be used to } } } } } stabilize the mixture befor centrifugation? } } } } } Thanks a lot -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859
==============================Original Headers============================== 2, 23 -- From oshel1pe-at-cmich.edu Wed Jun 20 10:32:15 2007 2, 23 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5KFWEu8029653 2, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 10:32:14 -0500 2, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 2, 23 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id l5KFsEqo009235 2, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 11:54:15 -0400 2, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 2, 23 -- Wed, 20 Jun 2007 11:32:12 -0400 2, 23 -- Mime-Version: 1.0 2, 23 -- Message-Id: {f06240807c29ef8a4d8ae-at-[141.209.160.249]} 2, 23 -- Date: Wed, 20 Jun 2007 11:32:09 -0400 2, 23 -- To: Microscopy-at-microscopy.com 2, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 2, 23 -- Subject: Fwd: Re: Fwd: [Microscopy] viaWWW: colloidal gold conjugation of 2, 23 -- proteins 2, 23 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 2, 23 -- X-OriginalArrivalTime: 20 Jun 2007 15:32:12.0260 (UTC) FILETIME=[2C231240:01C7B350] 2, 23 -- X-CanItPRO-Stream: default 2, 23 -- X-Spam-Score: -4 () L_EXCH_MF 2, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 2, 23 -- Content-Transfer-Encoding: 8bit 2, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5KFWEu8029653 ==============================End of - Headers==============================
Yes, there is no problem running at any kV one desires.
Furthermore the quality of images at higher voltages is far superior due to the better coherence of the el. beam for the LaB6 cathode and decreased inelastic scattering compared to tungsten and low voltage.
Also the need of lower voltage for improved contrast is questionable if the system is equipped with digital imaging capability. As long as resolution is there contrast can be improved quite significantly just by post-acquisition processing.
Radiolysis is the main problem causing specimen damage at voltages less than 300 kV and this increases with decreasing accelerating voltage. That is another advantage to operate at higher voltages.
The only drawback for having higher voltage instrument with LaB6 is the stricter vacuum requirements. For many life science applications this my cause some slowdown since pumping times will be longer during sample exchange. This can be resolved by obtaining multiple-grid specimen holders.
I would even suggest if funding is not a problem to go for a 300 kV instrument, provided you expect to do substantial work in materials science and geology.
Krassimir.
=================================== Krassimir N. Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
On Jun 20, 2007, at 5:27 AM, edelmare-at-Muohio.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Krassimir: } } Then you have no problem running any KeV at any time in your TEM(s)? } } } On 19 Jun 2007 at 10:02, K.N. Bozhilov wrote: } } } In an universal applications environment the only good reason to buy } } 120kV vs 200kV TEM is lack of money. } } } } =================================== } } Krassimir N. Bozhilov } } Central Facility for Advanced Microscopy and Microanalysis } } University of California } } Riverside, CA 92521 } } } } tel. 951 827 2998 } } fax 951 827 2489 } } =================================== } } } } } } On Jun 19, 2007, at 9:12 AM, edelmare-at-muohio.edu wrote: } } } } } } } } } } } } } } -------------------------------------------------------------------- } } } -- } } } ------ } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } } MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } -------------------------------------------------------------------- } } } -- } } } ------ } } } } } } 200Kev scope users: } } } } } } O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with } } } LaB6 guns. Our biggest concerns come in routine usability, and I am } } } hoping to hear any comments on any of the following questions we } } } have. } } } } } } We handle a wide range of samples (Biological thin sections, organic } } } macro-molecules, geologic particles, geologic thin sections, } } } crystals, thin films, nano-particles, organic and non-organic } } } materials) with a wide range of techniques (BF, DF, Diffraction, } } } EDS, } } } EELS, including lattice fringe imaging, STEM and tomography } } } secondarily). In balancing contrast, resolution, and beam damage we } } } really expect to perform a majority of our imaging between 80KeV and } } } 120KeV (or 150KeV). However, the option of higher voltages up to } } } 200KeV, for higher resolution imaging is something we are } } } considering. So we are looking at both 120 and 200 KeV scopes. } } } (And } } } yes, we will test as many samples on any scope as we can but a } } } couple } } } of days worth of playing with a handful of samples does not compare } } } to years worth of experience.) } } } } } } (1) It seems that most 200Kev scopes are set to 200Kev and left } } } there - presumably because it eases alignments, voltage stability, } } } etc. when pushing for ultimate resolution. Is this true? Yes, all } } } the manufacturers list voltage ranges the scope can be operated at, } } } but does anyone really use anything else? With the digitizing of } } } the } } } scope controls and storage of multiple alignment setting, alignment } } } at different voltages (theoretically) should be fairly straight } } } forward but is it really? } } } } } } (2) Is 200KeV worth having if the majority of the work does not } } } require 200KeV? Not having personally worked much at 200KeV, Does } } } loss of contrast and beam damage at 200KeV limit usability when } } } imaging other than atomic / lattice level? } } } } } } (3) If the majority of the scope usage is 80 - 140 KeV is the cost } } } of a 200KeV instrument worth it? In terms of initial cost, and } } } maintenance. (Even if the only option tops off at 120KeV). We are } } } all aware of instances of: "If you can at all afford it get, } } } even if } } } you won´t use it regularly, because . . ." is this one of those? } } } } } } (4) A few years ago the list had a discussion of W vs. LaB6, vs. } } } FEG/Schottky for SEM´s and the consensus basically was that LaB6 } } } was } } } not worth the cost for SEM. (Either FEG if affordable or tungsten } } } with better bells & whistles). Is LaB6 worth it in terms of TEM? } } } } } } (5) What more important issue have we neglected in our naivete? } } } } } } (Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?) } } } } } } Thank you for any info you're willing to share! } } } } } } } } } } } } Richard E. Edelmann, Ph.D. } } } EXPO Editor, Microscopy and Microanalysis Supplement } } } Electron Microscopy Facility Director } } } 364 Pearson Hall } } } Miami University, Oxford, OH 45056 } } } Ph: 513.529.5712 Fax: 513.529.4243 } } } E-mail: edelmare-at-muohio.edu } } } http://www.emf.muohio.edu } } } } } } } } } } } } ==============================Original } } } Headers============================== } } } 15, 23 -- From edelmare-at-muohio.edu Tue Jun 19 11:09:28 2007 } } } 15, 23 -- Received: from mulnx11.mcs.muohio.edu } } } (mulnx11.mcs.muohio.edu [134.53.6.66]) } } } 15, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } } ESMTP id l5JG9S9P012178 } } } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 } } } 11:09:28 -0500 } } } 15, 23 -- Received: from mulnx23.mcs.muohio.edu } } } (mulnx23.mcs.muohio.edu [134.53.6.10]) } } } 15, 23 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } } } with ESMTP id l5JG9Sda025021 } } } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 } } } 12:09:28 -0400 } } } 15, 23 -- Received: from [192.168.1.23] ([134.53.14.105]) } } } 15, 23 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } } } with ESMTP id l5JG9R7T014789 } } } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 } } } 12:09:27 -0400 } } } 15, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} } } } 15, 23 -- To: microscopy-at-Microscopy.com } } } 15, 23 -- Date: Tue, 19 Jun 2007 12:09:26 -0400 } } } 15, 23 -- MIME-Version: 1.0 } } } 15, 23 -- Subject: 200KeV TEM users Opinions, please } } } 15, 23 -- Message-ID: {4677C776.26865.13BE4E36-at-edelmare.muohio.edu} } } } 15, 23 -- Priority: normal } } } 15, 23 -- X-mailer: Pegasus Mail for Windows (4.41) } } } 15, 23 -- Content-type: text/plain; charset=ISO-8859-1 } } } 15, 23 -- Content-description: Mail message body } } } 15, 23 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 } } } 15, 23 -- Content-Transfer-Encoding: 8bit } } } 15, 23 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by } } } ns.microscopy.com id l5JG9S9P012178 } } } ==============================End of - } } } Headers============================== } } } } } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Director } 364 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } http://www.emf.muohio.edu } } "RAM disk is NOT an installation procedure." } } } } ==============================Original } Headers============================== } 10, 25 -- From edelmare-at-Muohio.edu Wed Jun 20 07:23:44 2007 } 10, 25 -- Received: from mulnx11.mcs.muohio.edu } (mulnx11.mcs.muohio.edu [134.53.6.66]) } 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l5KCNi18032133 } 10, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 } 07:23:44 -0500 } 10, 25 -- Received: from mulnx24.mcs.muohio.edu } (mulnx24.mcs.muohio.edu [134.53.6.11]) } 10, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } with ESMTP id l5KCKQud008353; } 10, 25 -- Wed, 20 Jun 2007 08:20:26 -0400 } 10, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) } 10, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } with ESMTP id l5KCKPCw001786; } 10, 25 -- Wed, 20 Jun 2007 08:20:25 -0400 } 10, 25 -- From: "Richard E. Edelmann" {edelmare-at-Muohio.edu} } 10, 25 -- To: "K.N. Bozhilov" {bozhilov-at-ucr.edu} , } microscopy-at-microscopy.com } 10, 25 -- Date: Wed, 20 Jun 2007 08:20:25 -0400 } 10, 25 -- MIME-Version: 1.0 } 10, 25 -- Subject: Re: [Microscopy] 200KeV TEM users Opinions, please } 10, 25 -- Message-ID: {4678E349.30182.1812C59F-at-edelmare.muohio.edu} } 10, 25 -- Priority: normal } 10, 25 -- In-reply-to: {B215E817-DE93-4016-9941-AF6F8CE7CD9B-at-ucr.edu} } 10, 25 -- References: } {200706191612.l5JGCCp6015586-at-ns.microscopy.com} , {B215E817- } DE93-4016-9941-AF6F8CE7CD9B-at-ucr.edu} } 10, 25 -- X-mailer: Pegasus Mail for Windows (4.41) } 10, 25 -- Content-type: text/plain; charset=ISO-8859-1 } 10, 25 -- Content-description: Mail message body } 10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 } 10, 25 -- Content-Transfer-Encoding: 8bit } 10, 25 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by } ns.microscopy.com id l5KCNi18032133 } ==============================End of - } Headers==============================
==============================Original Headers============================== 15, 22 -- From bozhilov-at-ucr.edu Wed Jun 20 10:56:32 2007 15, 22 -- Received: from sentoku.ucr.edu (sentoku.ucr.edu [138.23.226.244]) 15, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5KFuW3h009477 15, 22 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 10:56:32 -0500 15, 22 -- Received: from [138.23.185.162] (igppb116g.ucr.edu [138.23.185.162]) 15, 22 -- by sentoku.ucr.edu (MOS 3.6.6-GR) 15, 22 -- with ESMTP id BMP59564 (AUTH via LOGINBEFORESMTP); 15, 22 -- Wed, 20 Jun 2007 08:56:30 -0700 (PDT) 15, 22 -- In-Reply-To: {200706201227.l5KCRS6r003118-at-ns.microscopy.com} 15, 22 -- References: {200706201227.l5KCRS6r003118-at-ns.microscopy.com} 15, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 15, 22 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 15, 22 -- Message-Id: {424AF885-C093-44A7-8811-43176C225FBE-at-ucr.edu} 15, 22 -- Cc: microscopy-at-microscopy.com 15, 22 -- From: "K.N. Bozhilov" {bozhilov-at-ucr.edu} 15, 22 -- Subject: Re: [Microscopy] Re: 200KeV TEM users Opinions, please 15, 22 -- Date: Wed, 20 Jun 2007 08:56:29 -0700 15, 22 -- To: edelmare-at-Muohio.edu 15, 22 -- X-Mailer: Apple Mail (2.752.2) 15, 22 -- X-Junkmail-Whitelist: YES (by domain whitelist at sentoku.ucr.edu) 15, 22 -- Content-Transfer-Encoding: 8bit 15, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5KFuW3h009477 ==============================End of - Headers==============================
I am no lawyer but there are 3 facts that I have heard repeatedly.
First, if one doesn't patent (or start the process) within 12 months of it being published, it can't be.
Second, a patent only gives someone the right to sue to try and prove they own the rights. A court case can invalidate it - in other words, getting a patent isn't considered proof that you deserved it.
Third, one can't patent a process that anyone working in the field would or could do without any special thought or design. Karnovsky's idea to combine paraformaldehyde and glutaraldehyde probably wasn't patentable.
If someone comes up with a new fixative using a novel chemical, that probably would be patentable.
But I am curious, what fixative and where did you see it was patented.
At 09:47 AM 06/20/07, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
Richard, Krassimir; Please let me contribute one aspect to this thread that has not been considered: unless you purchase a TEM with constant power lenses, and I am not aware of any other than the FEI Titan, you will have a lengthy period of thermal instability following an accelerating voltage change because the lens currents change significantly. Dropping accelerating voltage can result in over-cooling the column, which can result in condensation, vacuum leaks and thermal drift, even if the water flow is adjusted. Raising the accelerating voltage after an extended period of low-voltage operation can result in a need to condition the accelerator to avoid HT instability. Those are some of the other reasons that TEMs generally run at their rated high voltage.
John Mardinly Intel Corporation
This comment does not represent an opinion of Intel Corporation.
-----Original Message----- X-from: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu] Sent: Wednesday, June 20, 2007 8:57 AM To: Mardinly, John
Richard,
Yes, there is no problem running at any kV one desires.
Furthermore the quality of images at higher voltages is far superior due to the better coherence of the el. beam for the LaB6 cathode and decreased inelastic scattering compared to tungsten and low voltage.
Also the need of lower voltage for improved contrast is questionable if the system is equipped with digital imaging capability. As long as resolution is there contrast can be improved quite significantly just by post-acquisition processing.
Radiolysis is the main problem causing specimen damage at voltages less than 300 kV and this increases with decreasing accelerating voltage. That is another advantage to operate at higher voltages.
The only drawback for having higher voltage instrument with LaB6 is the stricter vacuum requirements. For many life science applications this my cause some slowdown since pumping times will be longer during sample exchange. This can be resolved by obtaining multiple-grid specimen holders.
I would even suggest if funding is not a problem to go for a 300 kV instrument, provided you expect to do substantial work in materials science and geology.
Krassimir.
=================================== Krassimir N. Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
On Jun 20, 2007, at 5:27 AM, edelmare-at-Muohio.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Krassimir: } } Then you have no problem running any KeV at any time in your TEM(s)? } } } On 19 Jun 2007 at 10:02, K.N. Bozhilov wrote: } } } In an universal applications environment the only good reason to buy } } 120kV vs 200kV TEM is lack of money. } } } } =================================== } } Krassimir N. Bozhilov } } Central Facility for Advanced Microscopy and Microanalysis } } University of California } } Riverside, CA 92521 } } } } tel. 951 827 2998 } } fax 951 827 2489 } } =================================== } } } } } } On Jun 19, 2007, at 9:12 AM, edelmare-at-muohio.edu wrote: } } } } } } } } } } } } } } -------------------------------------------------------------------- } } } -- } } } ------ } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } } MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } -------------------------------------------------------------------- } } } -- } } } ------ } } } } } } 200Kev scope users: } } } } } } O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with } } } LaB6 guns. Our biggest concerns come in routine usability, and I am } } } hoping to hear any comments on any of the following questions we } } } have. } } } } } } We handle a wide range of samples (Biological thin sections, organic } } } macro-molecules, geologic particles, geologic thin sections, } } } crystals, thin films, nano-particles, organic and non-organic } } } materials) with a wide range of techniques (BF, DF, Diffraction, } } } EDS, } } } EELS, including lattice fringe imaging, STEM and tomography } } } secondarily). In balancing contrast, resolution, and beam damage we } } } really expect to perform a majority of our imaging between 80KeV and } } } 120KeV (or 150KeV). However, the option of higher voltages up to } } } 200KeV, for higher resolution imaging is something we are } } } considering. So we are looking at both 120 and 200 KeV scopes. } } } (And } } } yes, we will test as many samples on any scope as we can but a } } } couple } } } of days worth of playing with a handful of samples does not compare } } } to years worth of experience.) } } } } } } (1) It seems that most 200Kev scopes are set to 200Kev and left } } } there - presumably because it eases alignments, voltage stability, } } } etc. when pushing for ultimate resolution. Is this true? Yes, all } } } the manufacturers list voltage ranges the scope can be operated at, } } } but does anyone really use anything else? With the digitizing of } } } the } } } scope controls and storage of multiple alignment setting, alignment } } } at different voltages (theoretically) should be fairly straight } } } forward but is it really? } } } } } } (2) Is 200KeV worth having if the majority of the work does not } } } require 200KeV? Not having personally worked much at 200KeV, Does } } } loss of contrast and beam damage at 200KeV limit usability when } } } imaging other than atomic / lattice level? } } } } } } (3) If the majority of the scope usage is 80 - 140 KeV is the cost } } } of a 200KeV instrument worth it? In terms of initial cost, and } } } maintenance. (Even if the only option tops off at 120KeV). We are } } } all aware of instances of: "If you can at all afford it get, } } } even if } } } you won´t use it regularly, because . . ." is this one of those? } } } } } } (4) A few years ago the list had a discussion of W vs. LaB6, vs. } } } FEG/Schottky for SEM´s and the consensus basically was that LaB6 } } } was } } } not worth the cost for SEM. (Either FEG if affordable or tungsten } } } with better bells & whistles). Is LaB6 worth it in terms of TEM? } } } } } } (5) What more important issue have we neglected in our naivete? } } } } } } (Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?) } } } } } } Thank you for any info you're willing to share! } } } } } } } } } } } } Richard E. Edelmann, Ph.D. } } } EXPO Editor, Microscopy and Microanalysis Supplement } } } Electron Microscopy Facility Director } } } 364 Pearson Hall } } } Miami University, Oxford, OH 45056 } } } Ph: 513.529.5712 Fax: 513.529.4243 } } } E-mail: edelmare-at-muohio.edu } } } http://www.emf.muohio.edu } } } } } } } } } } } } ==============================Original } } } Headers============================== } } } 15, 23 -- From edelmare-at-muohio.edu Tue Jun 19 11:09:28 2007 } } } 15, 23 -- Received: from mulnx11.mcs.muohio.edu } } } (mulnx11.mcs.muohio.edu [134.53.6.66]) } } } 15, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } } ESMTP id l5JG9S9P012178 } } } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 } } } 11:09:28 -0500 } } } 15, 23 -- Received: from mulnx23.mcs.muohio.edu } } } (mulnx23.mcs.muohio.edu [134.53.6.10]) } } } 15, 23 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } } } with ESMTP id l5JG9Sda025021 } } } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 } } } 12:09:28 -0400 } } } 15, 23 -- Received: from [192.168.1.23] ([134.53.14.105]) } } } 15, 23 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } } } with ESMTP id l5JG9R7T014789 } } } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 } } } 12:09:27 -0400 } } } 15, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} } } } 15, 23 -- To: microscopy-at-Microscopy.com } } } 15, 23 -- Date: Tue, 19 Jun 2007 12:09:26 -0400 } } } 15, 23 -- MIME-Version: 1.0 } } } 15, 23 -- Subject: 200KeV TEM users Opinions, please } } } 15, 23 -- Message-ID: {4677C776.26865.13BE4E36-at-edelmare.muohio.edu} } } } 15, 23 -- Priority: normal } } } 15, 23 -- X-mailer: Pegasus Mail for Windows (4.41) } } } 15, 23 -- Content-type: text/plain; charset=ISO-8859-1 } } } 15, 23 -- Content-description: Mail message body } } } 15, 23 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 } } } 15, 23 -- Content-Transfer-Encoding: 8bit } } } 15, 23 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by } } } ns.microscopy.com id l5JG9S9P012178 } } } ==============================End of - } } } Headers============================== } } } } } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Director } 364 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } http://www.emf.muohio.edu } } "RAM disk is NOT an installation procedure." } } } } ==============================Original } Headers============================== } 10, 25 -- From edelmare-at-Muohio.edu Wed Jun 20 07:23:44 2007 } 10, 25 -- Received: from mulnx11.mcs.muohio.edu } (mulnx11.mcs.muohio.edu [134.53.6.66]) } 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l5KCNi18032133 } 10, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 } 07:23:44 -0500 } 10, 25 -- Received: from mulnx24.mcs.muohio.edu } (mulnx24.mcs.muohio.edu [134.53.6.11]) } 10, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } with ESMTP id l5KCKQud008353; } 10, 25 -- Wed, 20 Jun 2007 08:20:26 -0400 } 10, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) } 10, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } with ESMTP id l5KCKPCw001786; } 10, 25 -- Wed, 20 Jun 2007 08:20:25 -0400 } 10, 25 -- From: "Richard E. Edelmann" {edelmare-at-Muohio.edu} } 10, 25 -- To: "K.N. Bozhilov" {bozhilov-at-ucr.edu} , } microscopy-at-microscopy.com } 10, 25 -- Date: Wed, 20 Jun 2007 08:20:25 -0400 } 10, 25 -- MIME-Version: 1.0 } 10, 25 -- Subject: Re: [Microscopy] 200KeV TEM users Opinions, please } 10, 25 -- Message-ID: {4678E349.30182.1812C59F-at-edelmare.muohio.edu} } 10, 25 -- Priority: normal } 10, 25 -- In-reply-to: {B215E817-DE93-4016-9941-AF6F8CE7CD9B-at-ucr.edu} } 10, 25 -- References: } {200706191612.l5JGCCp6015586-at-ns.microscopy.com} , {B215E817- } DE93-4016-9941-AF6F8CE7CD9B-at-ucr.edu} } 10, 25 -- X-mailer: Pegasus Mail for Windows (4.41) } 10, 25 -- Content-type: text/plain; charset=ISO-8859-1 } 10, 25 -- Content-description: Mail message body } 10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 } 10, 25 -- Content-Transfer-Encoding: 8bit } 10, 25 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by } ns.microscopy.com id l5KCNi18032133 } ==============================End of - } Headers==============================
==============================Original Headers============================== 15, 22 -- From bozhilov-at-ucr.edu Wed Jun 20 10:56:32 2007 15, 22 -- Received: from sentoku.ucr.edu (sentoku.ucr.edu [138.23.226.244]) 15, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5KFuW3h009477 15, 22 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 10:56:32 -0500 15, 22 -- Received: from [138.23.185.162] (igppb116g.ucr.edu [138.23.185.162]) 15, 22 -- by sentoku.ucr.edu (MOS 3.6.6-GR) 15, 22 -- with ESMTP id BMP59564 (AUTH via LOGINBEFORESMTP); 15, 22 -- Wed, 20 Jun 2007 08:56:30 -0700 (PDT) 15, 22 -- In-Reply-To: {200706201227.l5KCRS6r003118-at-ns.microscopy.com} 15, 22 -- References: {200706201227.l5KCRS6r003118-at-ns.microscopy.com} 15, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 15, 22 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 15, 22 -- Message-Id: {424AF885-C093-44A7-8811-43176C225FBE-at-ucr.edu} 15, 22 -- Cc: microscopy-at-microscopy.com 15, 22 -- From: "K.N. Bozhilov" {bozhilov-at-ucr.edu} 15, 22 -- Subject: Re: [Microscopy] Re: 200KeV TEM users Opinions, please 15, 22 -- Date: Wed, 20 Jun 2007 08:56:29 -0700 15, 22 -- To: edelmare-at-Muohio.edu 15, 22 -- X-Mailer: Apple Mail (2.752.2) 15, 22 -- X-Junkmail-Whitelist: YES (by domain whitelist at sentoku.ucr.edu) 15, 22 -- Content-Transfer-Encoding: 8bit 15, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5KFuW3h009477 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 34 -- From john.mardinly-at-intel.com Wed Jun 20 14:52:32 2007 24, 34 -- Received: from mga03.intel.com (mga03.intel.com [143.182.124.21]) 24, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5KJqVlO005646 24, 34 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 20 Jun 2007 14:52:31 -0500 24, 34 -- Received: from azsmga001.ch.intel.com ([10.2.17.19]) 24, 34 -- by azsmga101.ch.intel.com with ESMTP; 20 Jun 2007 12:52:30 -0700 24, 34 -- X-ExtLoop1: 1 24, 34 -- X-IronPort-AV: E=Sophos;i="4.16,444,1175497200"; 24, 34 -- d="scan'208";a="241397258" 24, 34 -- Received: from fmsmsx333.amr.corp.intel.com ([132.233.42.2]) 24, 34 -- by azsmga001.ch.intel.com with ESMTP; 20 Jun 2007 12:52:30 -0700 24, 34 -- Received: from scsmsx412.amr.corp.intel.com ([10.3.90.31]) by fmsmsx333.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 24, 34 -- Wed, 20 Jun 2007 12:52:30 -0700 24, 34 -- Received: from scsmsx415.amr.corp.intel.com ([10.3.90.34]) by scsmsx412.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 24, 34 -- Wed, 20 Jun 2007 12:52:29 -0700 24, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 24, 34 -- Content-class: urn:content-classes:message 24, 34 -- MIME-Version: 1.0 24, 34 -- Content-Type: text/plain; 24, 34 -- charset="iso-8859-1" 24, 34 -- Subject: RE: [Microscopy] 200KeV TEM users Opinions, please 24, 34 -- Date: Wed, 20 Jun 2007 12:52:28 -0700 24, 34 -- Message-ID: {F3CB8931ABF8294DB889E977150CAD68A9AA40-at-scsmsx415.amr.corp.intel.com} 24, 34 -- In-Reply-To: {200706201556.l5KFudbj009591-at-ns.microscopy.com} 24, 34 -- X-MS-Has-Attach: 24, 34 -- X-MS-TNEF-Correlator: 24, 34 -- Thread-Topic: [Microscopy] 200KeV TEM users Opinions, please 24, 34 -- Thread-Index: AcezU5oHgFxv/ZDlSUKqiJvVM0YIawAHylMg 24, 34 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 24, 34 -- To: {bozhilov-at-ucr.edu} 24, 34 -- Cc: {Microscopy-at-msa.microscopy.com} 24, 34 -- X-OriginalArrivalTime: 20 Jun 2007 19:52:29.0583 (UTC) FILETIME=[88C971F0:01C7B374] 24, 34 -- Content-Transfer-Encoding: 8bit 24, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5KJqVlO005646 ==============================End of - Headers==============================
Greetings: We are looking to obtain/purchase a used ethane disposal system for a Reichert KF-80 cryo-freezing unit. The vendor is no longer able to supply one directly, so we are looking into the used market. If you have an ethane disposal system that you no longer use and would like to get rid of it, please send me a quick e-mail so we can discuss off-line: waheeschen-at-dow.com Best Regards,
Bill
William A. Heeschen, Ph.D. Microscopy, Digital Imaging The Dow Chemical Company Midland, MI 48674 waheeschen-at-dow.com
==============================Original Headers============================== 4, 26 -- From WAHeeschen-at-dow.com Wed Jun 20 14:58:19 2007 4, 26 -- Received: from mail87.messagelabs.com (mail87.messagelabs.com [216.82.250.19]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5KJwIxi012258 4, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 14:58:18 -0500 4, 26 -- X-VirusChecked: Checked 4, 26 -- X-Env-Sender: WAHeeschen-at-dow.com 4, 26 -- X-Msg-Ref: server-11.tower-87.messagelabs.com!1182369497!39708880!1 4, 26 -- X-StarScan-Version: 5.5.12.11; banners=-,-,- 4, 26 -- X-Originating-IP: [216.99.65.28] 4, 26 -- Received: (qmail 9609 invoked from network); 20 Jun 2007 19:58:17 -0000 4, 26 -- Received: from mail8.dow.com (HELO mante89.nam.dow.com) (216.99.65.28) 4, 26 -- by server-11.tower-87.messagelabs.com with SMTP; 20 Jun 2007 19:58:17 -0000 4, 26 -- Received: by mante89.nam.dow.com with Internet Mail Service (5.5.2658.3) 4, 26 -- id {M5YYNWCW} ; Wed, 20 Jun 2007 15:58:16 -0400 4, 26 -- Message-ID: {9FAC91DDE67EF3448238E5E33D3E5AEF3E7C1F-at-USMDLMDOWX026.dow.com} 4, 26 -- From: "Heeschen, Bill (WA)" {WAHeeschen-at-dow.com} 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- Subject: Looking for parts for Reichert KF-80 cryo-freezer 4, 26 -- Date: Wed, 20 Jun 2007 15:57:42 -0400 4, 26 -- MIME-Version: 1.0 4, 26 -- X-Mailer: Internet Mail Service (5.5.2658.3) 4, 26 -- content-class: urn:content-classes:message 4, 26 -- x-mimeole: Produced By Microsoft Exchange V6.5 4, 26 -- x-originalarrivaltime: 20 Jun 2007 19:57:43.0370 (UTC) FILETIME=[43D18AA0:01C7B375] 4, 26 -- Content-Type: text/plain; 4, 26 -- charset="iso-8859-1" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sharon.goresh-at-basf.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sharon.goresh-at-basf.com Name: Sharon L. Goresh
Organization: BASF Catalysts LLC
Title-Subject: [Filtered] Open Position BASF
Question: Chemist ñ 0700864
Description - Materials Characterization supports BASF Catalysts R&D by providing characterization, solving technical problems and participating in a wide range of R&D projects. ÝThis position is vital in our efforts in the key areas of electron microscopy (EPMA and SEM) and metallographic and ceramic sample preparation.
The candidate will spend the majority of their time working in the EPMA lab and the remainder in the sample preparation area. ÝThe successful candidate, with training as needed, will:
1. Prepare samples and operate the Cameca SX-50 Electron Microprobe. Ý 2. Acquire and process data using UNIX and PC based computers 3. Communicate with customers to assess appropriate analytical techniques. 4. Interpret data and issue written reports of the results.
Qualifications ñ Position requires a BA/BS degree in a scientific discipline with 1-2 years industrial or academic research laboratory experience. ÝHands-on experience using electron microscopes with X-Ray microanalysis capabilities is a plus. ÝThe successful candidate must have a theoretical understanding of inorganic chemistry, materials science or mineralogy and an enthusiasm to learn new techniques. ÝHe/she should also have excellent communication skills, a talent for developing positive working relationships, and the ability to handle a high volume of requests and meet critical deadlines.
Profile - ÝLocation: New Jersey-Iselin ÝJob Type: Standard ÝShift: Day Job
Those interested must apply directly on-line by going to:
http://www.basf.com/careers/taleo/joblist.htm
enter the job number in the search field and then follow the instructions
D. Jones wrote: =============================================
OK, I've been looking around for a group that talks/works on/etc. older microscopes. But I have not been successful in finding such a group.
I have just picked up an old Olympus PMG metallograph. It is an absolutely fascinating piece of optical equipment. I've never seen one like it.
Anyway, as one of my (ever increasing number of) extra-curricula activities, I've decided I'd like to either restore this instrument, or alter it so that I can actually use it as a modern metallograph with digital imaging capabilities. The first option would be my first choice, but if I can't get the parts needed to do that, I'll shoot for the second option...
My question to all the extraordinary minds on this group, does anyone know of someone that works on old microscopes? Does anyone know of a society or a group that discusses them, has old technical information about them, things like that?
I apologize if this is way off topic for this group. Please respond directly to me as I doubt the responses would be of general interest. If anyone does wish for a list of what I find, I'll be happy to share it, just let me know.
==============================================
The New York Microscopical Society, see URL
http://www.nyms.org/
might be just what you need. The Society actually owns and maintains quite a collection of old microscopes, some going back well over 100 years. I would expect that someone associated with this collection of old microscopes might know who could help you.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
==============================Original Headers============================== 21, 25 -- From cgarber-at-2spi.com Wed Jun 20 21:24:29 2007 21, 25 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5L2OSSj015816 21, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 21:24:29 -0500 21, 25 -- Received: from webmail.idv.net (diskless6.external [209.120.196.50]) 21, 25 -- (authenticated bits=0) 21, 25 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id l5L2OSuJ010859 21, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 22:24:28 -0400 21, 25 -- X-IDV-FirstRcvd: diskless6.external [209.120.196.50] 21, 25 -- X-IDV-HELO: webmail.idv.net 21, 25 -- X-IDV-Authenticated-User: cgarber 21, 25 -- Received: from 81.19.44.195 (auth. user cgarber-at-mail.2spi.com) 21, 25 -- by webmail.idv.net with HTTP; Wed, 20 Jun 2007 21:24:28 -0500 21, 25 -- To: microscopy-at-microscopy.com 21, 25 -- Subject: Reply to "unusual request" 21, 25 -- Date: Wed, 20 Jun 2007 21:24:28 -0500 21, 25 -- X-Mailer: IlohaMail/0.9.0 (On: webmail.idv.net) 21, 25 -- Message-ID: {WwNT5ZmM.1182392668.7308860.cgarber-at-mail.2spi.com} 21, 25 -- From: {cgarber-at-2spi.com} 21, 25 -- Bounce-To: {cgarber-at-2spi.com} 21, 25 -- Errors-To: {cgarber-at-2spi.com} 21, 25 -- MIME-Version: 1.0 21, 25 -- Content-Type: text/plain; charset=ISO-8859-1 21, 25 -- Content-Transfer-Encoding: 8bit 21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5L2OSSj015816 ==============================End of - Headers==============================
I had an application a few years ago to a small group/association (in US). I met one of the founders who did a presentation on old scopes 3-4 years ago but don't recall the name (senior moment at an early age). I'll dig up the info and post it in the next week or so.
I also know of a few (retired) fellows that can and will work on scopes - I'll send a couple of names off-line. I restore one here or there for my own sake (old petrographic and biological ones) but don't have time for outside work.
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
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-----Original Message----- X-from: cgarber-at-2spi.com [mailto:cgarber-at-2spi.com] Sent: Wednesday, June 20, 2007 10:33 PM To: ph2-at-sprynet.com
D. Jones wrote: =============================================
OK, I've been looking around for a group that talks/works on/etc. older microscopes. But I have not been successful in finding such a group.
I have just picked up an old Olympus PMG metallograph. It is an absolutely fascinating piece of optical equipment. I've never seen one like it.
Anyway, as one of my (ever increasing number of) extra-curricula activities, I've decided I'd like to either restore this instrument, or alter it so that I can actually use it as a modern metallograph with digital imaging capabilities. The first option would be my first choice, but if I can't get the parts needed to do that, I'll shoot for the second option...
My question to all the extraordinary minds on this group, does anyone know of someone that works on old microscopes? Does anyone know of a society or a group that discusses them, has old technical information about them, things like that?
I apologize if this is way off topic for this group. Please respond directly to me as I doubt the responses would be of general interest. If anyone does wish for a list of what I find, I'll be happy to share it, just let me know.
==============================================
The New York Microscopical Society, see URL
http://www.nyms.org/
might be just what you need. The Society actually owns and maintains quite a collection of old microscopes, some going back well over 100 years. I would expect that someone associated with this collection of old microscopes might know who could help you.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
==============================Original Headers============================== 21, 25 -- From cgarber-at-2spi.com Wed Jun 20 21:24:29 2007 21, 25 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5L2OSSj015816 21, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 21:24:29 -0500 21, 25 -- Received: from webmail.idv.net (diskless6.external [209.120.196.50]) 21, 25 -- (authenticated bits=0) 21, 25 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id l5L2OSuJ010859 21, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 22:24:28 -0400 21, 25 -- X-IDV-FirstRcvd: diskless6.external [209.120.196.50] 21, 25 -- X-IDV-HELO: webmail.idv.net 21, 25 -- X-IDV-Authenticated-User: cgarber 21, 25 -- Received: from 81.19.44.195 (auth. user cgarber-at-mail.2spi.com) 21, 25 -- by webmail.idv.net with HTTP; Wed, 20 Jun 2007 21:24:28 -0500 21, 25 -- To: microscopy-at-microscopy.com 21, 25 -- Subject: Reply to "unusual request" 21, 25 -- Date: Wed, 20 Jun 2007 21:24:28 -0500 21, 25 -- X-Mailer: IlohaMail/0.9.0 (On: webmail.idv.net) 21, 25 -- Message-ID: {WwNT5ZmM.1182392668.7308860.cgarber-at-mail.2spi.com} 21, 25 -- From: {cgarber-at-2spi.com} 21, 25 -- Bounce-To: {cgarber-at-2spi.com} 21, 25 -- Errors-To: {cgarber-at-2spi.com} 21, 25 -- MIME-Version: 1.0 21, 25 -- Content-Type: text/plain; charset=ISO-8859-1 21, 25 -- Content-Transfer-Encoding: 8bit 21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5L2OSSj015816 ==============================End of - Headers==============================
==============================Original Headers============================== 33, 27 -- From ph2-at-sprynet.com Wed Jun 20 22:13:21 2007 33, 27 -- Received: from elasmtp-galgo.atl.sa.earthlink.net (elasmtp-galgo.atl.sa.earthlink.net [209.86.89.61]) 33, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5L3DLU9028460 33, 27 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 22:13:21 -0500 33, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 33, 27 -- s=dk20050327; d=sprynet.com; 33, 27 -- b=G1YkSbusV91O7ooYxsr3USLIMlsP4G/iGxmZQH7fVP869h8R2ocOli2jejCWg9SE; 33, 27 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-MimeOLE:In-Reply-To:Thread-Index:Message-ID:X-ELNK-Trace:X-Originating-IP; 33, 27 -- Received: from [68.77.206.25] (helo=user915fa8f284) 33, 27 -- by elasmtp-galgo.atl.sa.earthlink.net with asmtp (Exim 4.34) 33, 27 -- id 1I1D6m-00054H-Ed 33, 27 -- for microscopy-at-microscopy.com; Wed, 20 Jun 2007 23:13:20 -0400 33, 27 -- From: "Tony Havics" {ph2-at-sprynet.com} 33, 27 -- To: "Micrscopy Listserve" {microscopy-at-microscopy.com} 33, 27 -- Subject: RE: [Microscopy] Reply to "unusual request" 33, 27 -- Date: Wed, 20 Jun 2007 23:13:18 -0400 33, 27 -- MIME-Version: 1.0 33, 27 -- Content-Type: text/plain; 33, 27 -- charset="us-ascii" 33, 27 -- Content-Transfer-Encoding: 7bit 33, 27 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 33, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 33, 27 -- In-Reply-To: {200706210233.l5L2XKgd024373-at-ns.microscopy.com} 33, 27 -- Thread-Index: AcezrIiMFvnVyVUzRXqYFI6muFH0jwABH3qA 33, 27 -- Message-ID: {E1I1D6m-00054H-Ed-at-elasmtp-galgo.atl.sa.earthlink.net} 33, 27 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f9932334815af5d7433b53cedb6fd153ec350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 33, 27 -- X-Originating-IP: 68.77.206.25 ==============================End of - Headers==============================
My suggestion was to subscribe to the usenet. Specifically, sci.techniques.microscopy which has many full time microscope repair persons on-line.
I don't know if the poster did this. So far, they have not shown up on the usenet.
gary g.
At 06:28 PM 6/20/2007, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Wed Jun 20 23:25:33 2007 10, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5L4PXek008706 10, 20 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 23:25:33 -0500 10, 20 -- Message-Id: {200706210425.l5L4PXek008706-at-ns.microscopy.com} 10, 20 -- Received: (qmail 11848 invoked from network); 20 Jun 2007 21:25:33 -0700 10, 20 -- Received: by simscan 1.1.0 ppid: 11844, pid: 11845, t: 0.1265s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp4 with SMTP; 20 Jun 2007 21:25:32 -0700 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Wed, 20 Jun 2007 21:25:35 -0800 10, 20 -- To: cgarber-at-2spi.com 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] Reply to "unusual request" 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200706210228.l5L2SIeg018684-at-ns.microscopy.com} 10, 20 -- References: {200706210228.l5L2SIeg018684-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-134A384C ==============================End of - Headers==============================
Back at Argonne National Lab, we had a JEOL 4000 that was kept at 400kV to high resolution work. It was aligned by the service engineers at 100, 200, 300 and 400, so recall of alignments was a push of a few buttons. At lower voltages the lenses run less power and are much cooler but generally did not affect resolution for most work. To run at 400kV for HREM imaging the TEM had to sit overnight to thermally equilibrate. When needed it ran at 100, 200kV. If someone really needs high resolution stability, set the HT to the setting wanted the night before; this is easily handled by indicated the voltage wanted when someone signs up for their time. If a user really wants resolution they will remember to indicate the desired voltage. Condensation and vacuum leaks were not a problem.
Best regards, Roseann
Roseann Csencsits, PhD Donner TEM Facility Manager Lawrence Berkeley Lab Room 365 Donner 510-486-4548
On Jun 20, 2007, at 1:02 PM, john.mardinly-at-intel.com wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Richard, Krassimir; } Please let me contribute one aspect to this thread that has not } been considered: unless you purchase a TEM with constant power } lenses, and I am not aware of any other than the FEI Titan, you } will have a lengthy period of thermal instability following an } accelerating voltage change because the lens currents change } significantly. Dropping accelerating voltage can result in over- } cooling the column, which can result in condensation, vacuum leaks } and thermal drift, even if the water flow is adjusted. Raising the } accelerating voltage after an extended period of low-voltage } operation can result in a need to condition the accelerator to } avoid HT instability. Those are some of the other reasons that TEMs } generally run at their rated high voltage. } } John Mardinly } Intel Corporation } } This comment does not represent an opinion of Intel Corporation. } } -----Original Message----- } X-from: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu] } Sent: Wednesday, June 20, 2007 8:57 AM } To: Mardinly, John } Subject: [Microscopy] 200KeV TEM users Opinions, please } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Richard, } } Yes, there is no problem running at any kV one desires. } } Furthermore the quality of images at higher voltages is far superior } due to the better coherence of the el. beam for the LaB6 cathode and } decreased inelastic scattering compared to tungsten and low voltage. } } Also the need of lower voltage for improved contrast is questionable } if the system is equipped with digital imaging capability. As long as } resolution is there contrast can be improved quite significantly just } by post-acquisition processing. } } Radiolysis is the main problem causing specimen damage at voltages } less than 300 kV and this increases with decreasing accelerating } voltage. That is another advantage to operate at higher voltages. } } The only drawback for having higher voltage instrument with LaB6 is } the stricter vacuum requirements. } For many life science applications this my cause some slowdown since } pumping times will be longer during sample exchange. This can be } resolved by obtaining multiple-grid specimen holders. } } I would even suggest if funding is not a problem to go for a 300 kV } instrument, provided you expect to do substantial work in materials } science and geology. } } Krassimir. } } =================================== } Krassimir N. Bozhilov } Central Facility for Advanced Microscopy and Microanalysis } University of California } Riverside, CA 92521 } } tel. 951 827 2998 } fax 951 827 2489 } =================================== } } } On Jun 20, 2007, at 5:27 AM, edelmare-at-Muohio.edu wrote: } } } } } } } } } ------ } } } } Krassimir: } } } } Then you have no problem running any KeV at any time in your TEM(s)? } } } } } } On 19 Jun 2007 at 10:02, K.N. Bozhilov wrote: } } } } } In an universal applications environment the only good reason to buy } } } 120kV vs 200kV TEM is lack of money. } } } } } } =================================== } } } Krassimir N. Bozhilov } } } Central Facility for Advanced Microscopy and Microanalysis } } } University of California } } } Riverside, CA 92521 } } } } } } tel. 951 827 2998 } } } fax 951 827 2489 } } } =================================== } } } } } } } } } On Jun 19, 2007, at 9:12 AM, edelmare-at-muohio.edu wrote: } } } } } } } } } } } } } } } } } } } - } } } } ------ } } } } } } } } 200Kev scope users: } } } } } } } } O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with } } } } LaB6 guns. Our biggest concerns come in routine usability, and } } } } I am } } } } hoping to hear any comments on any of the following questions we } } } } have. } } } } } } } } We handle a wide range of samples (Biological thin sections, } } } } organic } } } } macro-molecules, geologic particles, geologic thin sections, } } } } crystals, thin films, nano-particles, organic and non-organic } } } } materials) with a wide range of techniques (BF, DF, Diffraction, } } } } EDS, } } } } EELS, including lattice fringe imaging, STEM and tomography } } } } secondarily). In balancing contrast, resolution, and beam } } } } damage we } } } } really expect to perform a majority of our imaging between 80KeV } } } } and } } } } 120KeV (or 150KeV). However, the option of higher voltages up to } } } } 200KeV, for higher resolution imaging is something we are } } } } considering. So we are looking at both 120 and 200 KeV scopes. } } } } (And } } } } yes, we will test as many samples on any scope as we can but a } } } } couple } } } } of days worth of playing with a handful of samples does not compare } } } } to years worth of experience.) } } } } } } } } (1) It seems that most 200Kev scopes are set to 200Kev and left } } } } there - presumably because it eases alignments, voltage stability, } } } } etc. when pushing for ultimate resolution. Is this true? Yes, all } } } } the manufacturers list voltage ranges the scope can be operated at, } } } } but does anyone really use anything else? With the digitizing of } } } } the } } } } scope controls and storage of multiple alignment setting, alignment } } } } at different voltages (theoretically) should be fairly straight } } } } forward but is it really? } } } } } } } } (2) Is 200KeV worth having if the majority of the work does not } } } } require 200KeV? Not having personally worked much at 200KeV, Does } } } } loss of contrast and beam damage at 200KeV limit usability when } } } } imaging other than atomic / lattice level? } } } } } } } } (3) If the majority of the scope usage is 80 - 140 KeV is the cost } } } } of a 200KeV instrument worth it? In terms of initial cost, and } } } } maintenance. (Even if the only option tops off at 120KeV). We } } } } are } } } } all aware of instances of: "If you can at all afford it get, } } } } even if } } } } you won´t use it regularly, because . . ." is this one of those? } } } } } } } } (4) A few years ago the list had a discussion of W vs. LaB6, vs. } } } } FEG/Schottky for SEM´s and the consensus basically was that LaB6 } } } } was } } } } not worth the cost for SEM. (Either FEG if affordable or tungsten } } } } with better bells & whistles). Is LaB6 worth it in terms of TEM? } } } } } } } } (5) What more important issue have we neglected in our naivete? } } } } } } } } (Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?) } } } } } } } } Thank you for any info you're willing to share! } } } } } } } } } } } } } } } } Richard E. Edelmann, Ph.D. } } } } EXPO Editor, Microscopy and Microanalysis Supplement } } } } Electron Microscopy Facility Director } } } } 364 Pearson Hall } } } } Miami University, Oxford, OH 45056 } } } } Ph: 513.529.5712 Fax: 513.529.4243 } } } } E-mail: edelmare-at-muohio.edu } } } } http://www.emf.muohio.edu } } } }
==============================Original Headers============================== 7, 27 -- From RCsencsits-at-lbl.gov Wed Jun 20 23:27:29 2007 7, 27 -- Received: from ironport2.lbl.gov (ironport2.lbl.gov [128.3.41.14]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5L4RTKb010352 7, 27 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 20 Jun 2007 23:27:29 -0500 7, 27 -- X-Ironport-SBRS: 3.5 7, 27 -- X-Brightmail-Tracker: AAAAAA== 7, 27 -- X-BrightmailFiltered: true 7, 27 -- X-IronPort-AV: E=Sophos;i="4.16,445,1175497200"; 7, 27 -- d="scan'208";a="21383767" 7, 27 -- Received: from mta1.lbl.gov ([128.3.41.24]) 7, 27 -- by ironport2.lbl.gov with ESMTP; 20 Jun 2007 21:27:28 -0700 7, 27 -- Received: from [10.0.1.2] (c-76-103-17-167.hsd1.ca.comcast.net [76.103.17.167]) 7, 27 -- by mta1.lbl.gov (8.13.8/8.13.8) with ESMTP id l5L4RQgi016805; 7, 27 -- Wed, 20 Jun 2007 21:27:26 -0700 (PDT) 7, 27 -- In-Reply-To: {200706202002.l5KK241N023168-at-ns.microscopy.com} 7, 27 -- References: {200706202002.l5KK241N023168-at-ns.microscopy.com} 7, 27 -- Mime-Version: 1.0 (Apple Message framework v752.3) 7, 27 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 7, 27 -- Message-Id: {F063B25E-AC81-402A-A7BE-D32E28694610-at-lbl.gov} 7, 27 -- Cc: edelmare-at-muohio.edu 7, 27 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov} 7, 27 -- Subject: Re: [Microscopy] RE: 200KeV TEM users Opinions, please 7, 27 -- Date: Wed, 20 Jun 2007 21:24:52 -0700 7, 27 -- To: Microscopy-at-msa.microscopy.com 7, 27 -- X-Mailer: Apple Mail (2.752.3) 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5L4RTKb010352 ==============================End of - Headers==============================
One of the options in it is to go to the usenet. However, your ISP must be plugged into the usenet to make this work. The syntax is usually
usenet.yourisp.com
some form it as
usereader.yourisp.com
In all cases, "yourisp" is the name for your ISP. I "think" that you can get to the groups via Yahoo and/or Google. I'm not sure. I use Agent and connect directly via my ISP.
If you have difficulty, I can post a usenet message for you and forward the responses. This is a very good group.
gary g.
At 09:02 PM 6/20/2007, you wrote:
} Gary, } } I tried to get onto the group you've mentioned, but have not been successful. } } I guess I don't know how to do this... I used to get on to these } usenet groups a number of years ago, but I seem to have lost the } ability to do so now with only a web browser available to me.... } } dj } } On Wed, 20 Jun 2007, gary-at-gaugler.com wrote: } } } Date: Wed, 20 Jun 2007 23:31:52 -0500 } } From: gary-at-gaugler.com } } To: dljones-at-bestweb.net } } Subject: [Microscopy] Re: Reply to "unusual request" } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 14, 21 -- From gary-at-gaugler.com Thu Jun 21 00:16:44 2007 14, 21 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 14, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5L5Gh2N002043 14, 21 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 00:16:44 -0500 14, 21 -- Message-Id: {200706210516.l5L5Gh2N002043-at-ns.microscopy.com} 14, 21 -- Received: (qmail 15078 invoked from network); 20 Jun 2007 22:16:43 -0700 14, 21 -- Received: by simscan 1.1.0 ppid: 15074, pid: 15075, t: 0.1892s 14, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 14, 21 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 14, 21 -- by qsmtp3 with SMTP; 20 Jun 2007 22:16:43 -0700 14, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 14, 21 -- Date: Wed, 20 Jun 2007 22:16:46 -0800 14, 21 -- To: "David L. Jones" {"dljones-at-bestweb.net"-at-bestweb.net} 14, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 14, 21 -- Subject: Re: [Microscopy] Re: Reply to "unusual request" 14, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 14, 21 -- In-Reply-To: {Pine.WNT.4.64.0706210100060.180-at-dljtoshiba} 14, 21 -- References: {200706210431.l5L4VqCp019541-at-ns.microscopy.com} 14, 21 -- {Pine.WNT.4.64.0706210100060.180-at-dljtoshiba} 14, 21 -- Mime-Version: 1.0 14, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-3FBC5C3E ==============================End of - Headers==============================
} Go download Netscape. } } http://www.netscape.com } } One of the options in it is to go to the usenet. } However, your ISP must be plugged into the usenet } to make this work. The syntax is usually } } usenet.yourisp.com } } some form it as } } usereader.yourisp.com } } In all cases, "yourisp" is the name for your ISP. } I "think" that you can get to the groups via } Yahoo and/or Google. I'm not sure. I use Agent } and connect directly via my ISP. } } If you have difficulty, I can post a usenet message } for you and forward the responses. This is a very } good group. } } gary g. } } } } At 09:02 PM 6/20/2007, you wrote: } } } Gary, } } } } I tried to get onto the group you've mentioned, but have not been successful. } } } } I guess I don't know how to do this... I used to get on to these } } usenet groups a number of years ago, but I seem to have lost the } } ability to do so now with only a web browser available to me.... } } } } dj } } } } On Wed, 20 Jun 2007, gary-at-gaugler.com wrote: } } } } } Date: Wed, 20 Jun 2007 23:31:52 -0500 } } } From: gary-at-gaugler.com } } } To: dljones-at-bestweb.net } } } Subject: [Microscopy] Re: Reply to "unusual request" } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- ----------------------------------------------------------------------- Scott D. Davilla Phone: 919 489-1757 ext 13 (tel) 4pi Analysis, Inc. Fax: 919 489-1487 (fax) 3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com Durham, North Carolina 27707-2534 web: http://www.4pi.com
==============================Original Headers============================== 5, 16 -- From davilla-at-4pi.com Thu Jun 21 00:41:23 2007 5, 16 -- Received: from mx.4pi.com (mx.4pi.com [24.172.19.59]) 5, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5L5fNe8014250 5, 16 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 00:41:23 -0500 5, 16 -- Received: from [192.168.2.44] (rbl.4pi.com [24.172.19.62]) 5, 16 -- by mx.4pi.com (Postfix) with ESMTP id 0F99A356BB4D 5, 16 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 01:43:12 -0400 (EDT) 5, 16 -- Mime-Version: 1.0 5, 16 -- Message-Id: {p06230921c29fbf9ffa0a-at-[192.168.2.44]} 5, 16 -- In-Reply-To: {200706210516.l5L5Gm4O002092-at-ns.microscopy.com} 5, 16 -- References: {200706210516.l5L5Gm4O002092-at-ns.microscopy.com} 5, 16 -- Date: Thu, 21 Jun 2007 01:40:35 -0400 5, 16 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 5, 16 -- From: "Scott D. Davilla" {davilla-at-4pi.com} 5, 16 -- Subject: Re: [Microscopy] Reply to "unusual request" 5, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Excellent... Thank you for the pointer. This is another option for accessing the usenet. It should work.
gary g.
At 09:43 PM 6/20/2007, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 6, 20 -- From gary-at-gaugler.com Thu Jun 21 00:52:40 2007 6, 20 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5L5qerW025887 6, 20 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 00:52:40 -0500 6, 20 -- Message-Id: {200706210552.l5L5qerW025887-at-ns.microscopy.com} 6, 20 -- Received: (qmail 3689 invoked from network); 20 Jun 2007 22:52:36 -0700 6, 20 -- Received: by simscan 1.1.0 ppid: 3685, pid: 3687, t: 0.2891s 6, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 6, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 6, 20 -- by qsmtp2 with SMTP; 20 Jun 2007 22:52:36 -0700 6, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 6, 20 -- Date: Wed, 20 Jun 2007 22:52:38 -0800 6, 20 -- To: davilla-at-4pi.com 6, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 6, 20 -- Subject: Re: [Microscopy] Re: Reply to "unusual request" 6, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 6, 20 -- In-Reply-To: {200706210543.l5L5h8MV017075-at-ns.microscopy.com} 6, 20 -- References: {200706210543.l5L5h8MV017075-at-ns.microscopy.com} 6, 20 -- Mime-Version: 1.0 6, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-5FD7AE9 ==============================End of - Headers==============================
That may sound stupid, but I would still try fixation at neutral pH! Would you expect the change of pH to have dramatic effects? In parallel you could still try to fix at pH 3. Surely glutaraldehyde won't be at its maximum efficiency but if you increase the fixation time, it'll probably work somehow. Just compare the results at both pHs. And please let us know what came out, so there is a chance that we will be less stupid tomorrow than yesterday.
Regards,
Stephane
--- tina-at-pbrc.hawaii.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Once upon a time I had to perform TEM on pineapples } of different stages of } ripeness, and I had problems that someone said was } because glutaraldehyde } doesn't fix well at low pH. (Nevermind the more } pressing problem was the } crystal of Si in each cell, and all that pineapple } juice...) Now I have } clients who want to fix bacteria that are being } cultured at a pH of about } 3.0. Do any of you have any suggestions for a } fixation protocol? Cryo is } not an option at this time. } } Mahalo, } Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * } tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) } 956-6251 * } * University of Hawaii at Manoa * } http://www.pbrc.hawaii.edu/bemf* } **************************************************************************** } } } ==============================Original } Headers============================== } 4, 19 -- From tina-at-pbrc.hawaii.edu Mon Jun 18 } 23:40:53 2007 } 4, 19 -- Received: from halia.pbrc.hawaii.edu } (halia.pbrc.hawaii.edu [128.171.22.7]) } 4, 19 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l5J4eqot027780 } 4, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, } 18 Jun 2007 23:40:53 -0500 } 4, 19 -- Received: from halia.pbrc.hawaii.edu } (localhost [127.0.0.1]) } 4, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) } with ESMTP id l5J4ekEe011567 } 4, 19 -- (version=TLSv1/SSLv3 } cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) } 4, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, } 18 Jun 2007 18:40:48 -1000 (HST) } 4, 19 -- Received: from localhost by } halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with } ESMTP id l5J4ekL6011564 } 4, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, } 18 Jun 2007 18:40:46 -1000 (HST) } 4, 19 -- X-Authentication-Warning: } halia.pbrc.hawaii.edu: tina owned process doing -bs } 4, 19 -- Date: Mon, 18 Jun 2007 18:40:45 -1000 (HST) } 4, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} } 4, 19 -- X-Sender: tina-at-halia } 4, 19 -- To: Microscopy Listserver } {Microscopy-at-MSA.Microscopy.Com} } 4, 19 -- Subject: Fixing low pH extremophiles } 4, 19 -- Message-ID: } {Pine.GSO.4.21.0706181835070.11482-100000-at-halia} } 4, 19 -- MIME-Version: 1.0 } 4, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII } ==============================End of - } Headers============================== }
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==============================Original Headers============================== 15, 21 -- From nizets2-at-yahoo.com Thu Jun 21 01:41:39 2007 15, 21 -- Received: from web37408.mail.mud.yahoo.com (web37408.mail.mud.yahoo.com [209.191.91.140]) 15, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5L6fdZN005691 15, 21 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 01:41:39 -0500 15, 21 -- Received: (qmail 27468 invoked by uid 60001); 21 Jun 2007 06:41:39 -0000 15, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 15, 21 -- s=s1024; d=yahoo.com; 15, 21 -- h=X-YMail-OSG:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 15, 21 -- b=GFuJBWqIazONEuqbpGgzixDyrjnghk1TExT7qgZeu3M0GFPZMJYOq/Qs2oaHyzb9Js+257xR0BHPgxCFYobgPg+rZQL2BWxiWkgzjXplDGb37KIs9GWOiKn3Ddqis72lNKwrWBS12nqlynLhrRQnBUwiO0tHRn1+bx2x5dv40Bs=; 15, 21 -- X-YMail-OSG: cYDRGLMVM1n60MMNK1GLBcMsq6PyUI5B6N9XPgkAEuzuSjK1mCB1JmuBRbps6UtiWhr2hwSueFQXbNaEVkqo4uoa0tZPczaBML6G_FbLbLmRVOXTEXI6.YfjYAewMdALcIosfFoWhEGQ1TTZp4Khcx.eMXJWyS5tCEe5vyhwwBPE 15, 21 -- Received: from [80.122.101.102] by web37408.mail.mud.yahoo.com via HTTP; Wed, 20 Jun 2007 23:41:39 PDT 15, 21 -- Date: Wed, 20 Jun 2007 23:41:39 -0700 (PDT) 15, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 15, 21 -- Subject: Re: [Microscopy] Fixing low pH extremophiles 15, 21 -- To: tina-at-pbrc.hawaii.edu 15, 21 -- Cc: microscopy-at-microscopy.com 15, 21 -- In-Reply-To: {200706190447.l5J4lCot003035-at-ns.microscopy.com} 15, 21 -- MIME-Version: 1.0 15, 21 -- Content-Type: text/plain; charset=iso-8859-1 15, 21 -- Content-Transfer-Encoding: 8bit 15, 21 -- Message-ID: {354004.24544.qm-at-web37408.mail.mud.yahoo.com} ==============================End of - Headers==============================
Uranyl acetate may be worth trying, since 1% water solutions have a pH around 3.8. Yet remarkable efficacy has been reported for UrAc at pH 7.2 as a primary fixative for whole tissue structure and immunolabeling by Fassel & Greaser 1997 (Microsc Res Tech 37:600-601)
and at unadjusted pH 3.8 as an agent for time-resolved fixation of fleeting intermediates during rapid structural transitions when used for negative staining by Zhao & Craig 2003 (J Struct Biol 141:43-52; J Mol Biol 327:145-58).
-mike reedy- } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
What you are pointing out is true in principle and in theory and it could affect seriously only dedicated HR TEM instruments.
I have experience with a Philips/FEI EM, CM and Tecnai TEMs. As an example I will point out to oue CM300 which for the past over 10 years has been operated routinely between 100, 200 and 300 kV. This machine has theoretical point resolution of 0.23 nm and we have never encountered problems with obtaining the specs for resolution and stability even when we had to switch between different voltages within several hours time span.
If you have a FEG instrument with objective lenses with small coefficient for spherical aberration then minor instabilities are prone to cause considerable consequences but on a W or LaB6 instrument with relatively large gap between the objective polepieces the problems are negligible.
In essence what I want to says that if one needs to operate an instrument at the highest possible resolution and stability then operating at the maximum voltage is probably what should be expected but for a machine designed and dedicated for universal application there are no serious problems with using range of voltages specifically with the FEI twin lens instrument which I have extended experience. I suspect that this would be true for JEOL, Hitachi and Zeiss but cannot confirm it from my own experience.
Krassimir. =================================== Krassimir N. Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
On Jun 20, 2007, at 12:52 PM, Mardinly, John wrote:
} Richard, Krassimir; } Please let me contribute one aspect to this thread that has not } been considered: unless you purchase a TEM with constant power } lenses, and I am not aware of any other than the FEI Titan, you } will have a lengthy period of thermal instability following an } accelerating voltage change because the lens currents change } significantly. Dropping accelerating voltage can result in over- } cooling the column, which can result in condensation, vacuum leaks } and thermal drift, even if the water flow is adjusted. Raising the } accelerating voltage after an extended period of low-voltage } operation can result in a need to condition the accelerator to } avoid HT instability. Those are some of the other reasons that TEMs } generally run at their rated high voltage. } } John Mardinly } Intel Corporation } } This comment does not represent an opinion of Intel Corporation. } } -----Original Message----- } From: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu] } Sent: Wednesday, June 20, 2007 8:57 AM } To: Mardinly, John } Subject: [Microscopy] 200KeV TEM users Opinions, please } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Richard, } } Yes, there is no problem running at any kV one desires. } } Furthermore the quality of images at higher voltages is far superior } due to the better coherence of the el. beam for the LaB6 cathode and } decreased inelastic scattering compared to tungsten and low voltage. } } Also the need of lower voltage for improved contrast is questionable } if the system is equipped with digital imaging capability. As long as } resolution is there contrast can be improved quite significantly just } by post-acquisition processing. } } Radiolysis is the main problem causing specimen damage at voltages } less than 300 kV and this increases with decreasing accelerating } voltage. That is another advantage to operate at higher voltages. } } The only drawback for having higher voltage instrument with LaB6 is } the stricter vacuum requirements. } For many life science applications this my cause some slowdown since } pumping times will be longer during sample exchange. This can be } resolved by obtaining multiple-grid specimen holders. } } I would even suggest if funding is not a problem to go for a 300 kV } instrument, provided you expect to do substantial work in materials } science and geology. } } Krassimir. } } =================================== } Krassimir N. Bozhilov } Central Facility for Advanced Microscopy and Microanalysis } University of California } Riverside, CA 92521 } } tel. 951 827 2998 } fax 951 827 2489 } =================================== } } } On Jun 20, 2007, at 5:27 AM, edelmare-at-Muohio.edu wrote: } } } } } } } } } --------------------------------------------------------------------- } } - } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------- } } - } } ------ } } } } Krassimir: } } } } Then you have no problem running any KeV at any time in your TEM(s)? } } } } } } On 19 Jun 2007 at 10:02, K.N. Bozhilov wrote: } } } } } In an universal applications environment the only good reason to buy } } } 120kV vs 200kV TEM is lack of money. } } } } } } =================================== } } } Krassimir N. Bozhilov } } } Central Facility for Advanced Microscopy and Microanalysis } } } University of California } } } Riverside, CA 92521 } } } } } } tel. 951 827 2998 } } } fax 951 827 2489 } } } =================================== } } } } } } } } } On Jun 19, 2007, at 9:12 AM, edelmare-at-muohio.edu wrote: } } } } } } } } } } } } } } } } } } } ------------------------------------------------------------------- } } } } - } } } } -- } } } } ------ } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } } America } } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } } } MicroscopyListserver } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/ } } } } FAQ.html } } } } ------------------------------------------------------------------- } } } } - } } } } -- } } } } ------ } } } } } } } } 200Kev scope users: } } } } } } } } O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with } } } } LaB6 guns. Our biggest concerns come in routine usability, and } } } } I am } } } } hoping to hear any comments on any of the following questions we } } } } have. } } } } } } } } We handle a wide range of samples (Biological thin sections, } } } } organic } } } } macro-molecules, geologic particles, geologic thin sections, } } } } crystals, thin films, nano-particles, organic and non-organic } } } } materials) with a wide range of techniques (BF, DF, Diffraction, } } } } EDS, } } } } EELS, including lattice fringe imaging, STEM and tomography } } } } secondarily). In balancing contrast, resolution, and beam } } } } damage we } } } } really expect to perform a majority of our imaging between 80KeV } } } } and } } } } 120KeV (or 150KeV). However, the option of higher voltages up to } } } } 200KeV, for higher resolution imaging is something we are } } } } considering. So we are looking at both 120 and 200 KeV scopes. } } } } (And } } } } yes, we will test as many samples on any scope as we can but a } } } } couple } } } } of days worth of playing with a handful of samples does not compare } } } } to years worth of experience.) } } } } } } } } (1) It seems that most 200Kev scopes are set to 200Kev and left } } } } there - presumably because it eases alignments, voltage stability, } } } } etc. when pushing for ultimate resolution. Is this true? Yes, all } } } } the manufacturers list voltage ranges the scope can be operated at, } } } } but does anyone really use anything else? With the digitizing of } } } } the } } } } scope controls and storage of multiple alignment setting, alignment } } } } at different voltages (theoretically) should be fairly straight } } } } forward but is it really? } } } } } } } } (2) Is 200KeV worth having if the majority of the work does not } } } } require 200KeV? Not having personally worked much at 200KeV, Does } } } } loss of contrast and beam damage at 200KeV limit usability when } } } } imaging other than atomic / lattice level? } } } } } } } } (3) If the majority of the scope usage is 80 - 140 KeV is the cost } } } } of a 200KeV instrument worth it? In terms of initial cost, and } } } } maintenance. (Even if the only option tops off at 120KeV). We } } } } are } } } } all aware of instances of: "If you can at all afford it get, } } } } even if } } } } you won´t use it regularly, because . . ." is this one of those? } } } } } } } } (4) A few years ago the list had a discussion of W vs. LaB6, vs. } } } } FEG/Schottky for SEM´s and the consensus basically was that LaB6 } } } } was } } } } not worth the cost for SEM. (Either FEG if affordable or tungsten } } } } with better bells & whistles). Is LaB6 worth it in terms of TEM? } } } } } } } } (5) What more important issue have we neglected in our naivete? } } } } } } } } (Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?) } } } } } } } } Thank you for any info you're willing to share! } } } } } } } } } } } } } } } } Richard E. Edelmann, Ph.D. } } } } EXPO Editor, Microscopy and Microanalysis Supplement } } } } Electron Microscopy Facility Director } } } } 364 Pearson Hall } } } } Miami University, Oxford, OH 45056 } } } } Ph: 513.529.5712 Fax: 513.529.4243 } } } } E-mail: edelmare-at-muohio.edu } } } } http://www.emf.muohio.edu } } } } } } } } } } } } } } } } ==============================Original } } } } Headers============================== } } } } 15, 23 -- From edelmare-at-muohio.edu Tue Jun 19 11:09:28 2007 } } } } 15, 23 -- Received: from mulnx11.mcs.muohio.edu } } } } (mulnx11.mcs.muohio.edu [134.53.6.66]) } } } } 15, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } } } ESMTP id l5JG9S9P012178 } } } } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 } } } } 11:09:28 -0500 } } } } 15, 23 -- Received: from mulnx23.mcs.muohio.edu } } } } (mulnx23.mcs.muohio.edu [134.53.6.10]) } } } } 15, 23 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } } } } with ESMTP id l5JG9Sda025021 } } } } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 } } } } 12:09:28 -0400 } } } } 15, 23 -- Received: from [192.168.1.23] ([134.53.14.105]) } } } } 15, 23 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } } } } with ESMTP id l5JG9R7T014789 } } } } 15, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 19 Jun 2007 } } } } 12:09:27 -0400 } } } } 15, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} } } } } 15, 23 -- To: microscopy-at-Microscopy.com } } } } 15, 23 -- Date: Tue, 19 Jun 2007 12:09:26 -0400 } } } } 15, 23 -- MIME-Version: 1.0 } } } } 15, 23 -- Subject: 200KeV TEM users Opinions, please } } } } 15, 23 -- Message-ID: {4677C776.26865.13BE4E36-at-edelmare.muohio.edu} } } } } 15, 23 -- Priority: normal } } } } 15, 23 -- X-mailer: Pegasus Mail for Windows (4.41) } } } } 15, 23 -- Content-type: text/plain; charset=ISO-8859-1 } } } } 15, 23 -- Content-description: Mail message body } } } } 15, 23 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 } } } } 15, 23 -- Content-Transfer-Encoding: 8bit } } } } 15, 23 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by } } } } ns.microscopy.com id l5JG9S9P012178 } } } } ==============================End of - } } } } Headers============================== } } } } } } } } } Richard E. Edelmann, Ph.D. } } Electron Microscopy Facility Director } } 364 Pearson Hall } } Miami University, Oxford, OH 45056 } } Ph: 513.529.5712 Fax: 513.529.4243 } } E-mail: edelmare-at-muohio.edu } } http://www.emf.muohio.edu } } } } "RAM disk is NOT an installation procedure." } } } } } } } } ==============================Original } } Headers============================== } } 10, 25 -- From edelmare-at-Muohio.edu Wed Jun 20 07:23:44 2007 } } 10, 25 -- Received: from mulnx11.mcs.muohio.edu } } (mulnx11.mcs.muohio.edu [134.53.6.66]) } } 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id l5KCNi18032133 } } 10, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 } } 07:23:44 -0500 } } 10, 25 -- Received: from mulnx24.mcs.muohio.edu } } (mulnx24.mcs.muohio.edu [134.53.6.11]) } } 10, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } } with ESMTP id l5KCKQud008353; } } 10, 25 -- Wed, 20 Jun 2007 08:20:26 -0400 } } 10, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) } } 10, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } } with ESMTP id l5KCKPCw001786; } } 10, 25 -- Wed, 20 Jun 2007 08:20:25 -0400 } } 10, 25 -- From: "Richard E. Edelmann" {edelmare-at-Muohio.edu} } } 10, 25 -- To: "K.N. Bozhilov" {bozhilov-at-ucr.edu} , } } microscopy-at-microscopy.com } } 10, 25 -- Date: Wed, 20 Jun 2007 08:20:25 -0400 } } 10, 25 -- MIME-Version: 1.0 } } 10, 25 -- Subject: Re: [Microscopy] 200KeV TEM users Opinions, please } } 10, 25 -- Message-ID: {4678E349.30182.1812C59F-at-edelmare.muohio.edu} } } 10, 25 -- Priority: normal } } 10, 25 -- In-reply-to: {B215E817-DE93-4016-9941-AF6F8CE7CD9B-at-ucr.edu} } } 10, 25 -- References: } } {200706191612.l5JGCCp6015586-at-ns.microscopy.com} , {B215E817- } } DE93-4016-9941-AF6F8CE7CD9B-at-ucr.edu} } } 10, 25 -- X-mailer: Pegasus Mail for Windows (4.41) } } 10, 25 -- Content-type: text/plain; charset=ISO-8859-1 } } 10, 25 -- Content-description: Mail message body } } 10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 } } 10, 25 -- Content-Transfer-Encoding: 8bit } } 10, 25 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by } } ns.microscopy.com id l5KCNi18032133 } } ==============================End of - } } Headers============================== } } } } ==============================Original } Headers============================== } 15, 22 -- From bozhilov-at-ucr.edu Wed Jun 20 10:56:32 2007 } 15, 22 -- Received: from sentoku.ucr.edu (sentoku.ucr.edu } [138.23.226.244]) } 15, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l5KFuW3h009477 } 15, 22 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jun 2007 } 10:56:32 -0500 } 15, 22 -- Received: from [138.23.185.162] (igppb116g.ucr.edu } [138.23.185.162]) } 15, 22 -- by sentoku.ucr.edu (MOS 3.6.6-GR) } 15, 22 -- with ESMTP id BMP59564 (AUTH via LOGINBEFORESMTP); } 15, 22 -- Wed, 20 Jun 2007 08:56:30 -0700 (PDT) } 15, 22 -- In-Reply-To: {200706201227.l5KCRS6r003118-at-ns.microscopy.com} } 15, 22 -- References: {200706201227.l5KCRS6r003118-at-ns.microscopy.com} } 15, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) } 15, 22 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; } format=flowed } 15, 22 -- Message-Id: {424AF885-C093-44A7-8811-43176C225FBE-at-ucr.edu} } 15, 22 -- Cc: microscopy-at-microscopy.com } 15, 22 -- From: "K.N. 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==============================Original Headers============================== 12, 22 -- From bozhilov-at-ucr.edu Thu Jun 21 10:34:43 2007 12, 22 -- Received: from sentry.ucr.edu (sentry.ucr.edu [138.23.226.224]) 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5LFYhhQ030852 12, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 21 Jun 2007 10:34:43 -0500 12, 22 -- Received: from [138.23.185.162] (igppb116g.ucr.edu [138.23.185.162]) 12, 22 -- by sentry.ucr.edu (MOS 3.6.6-GR) 12, 22 -- with ESMTP id EUU69905 (AUTH via LOGINBEFORESMTP); 12, 22 -- Thu, 21 Jun 2007 08:34:39 -0700 (PDT) 12, 22 -- In-Reply-To: {F3CB8931ABF8294DB889E977150CAD68A9AA40-at-scsmsx415.amr.corp.intel.com} 12, 22 -- References: {F3CB8931ABF8294DB889E977150CAD68A9AA40-at-scsmsx415.amr.corp.intel.com} 12, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 12, 22 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 12, 22 -- Message-Id: {4F2E72EA-4942-4D5A-A8AE-93192B3717A4-at-ucr.edu} 12, 22 -- Cc: {Microscopy-at-MSA.Microscopy.Com} 12, 22 -- From: "K.N. Bozhilov" {bozhilov-at-ucr.edu} 12, 22 -- Subject: Re: [Microscopy] 200KeV TEM users Opinions, please 12, 22 -- Date: Thu, 21 Jun 2007 08:34:38 -0700 12, 22 -- To: "Mardinly, John" {john.mardinly-at-intel.com} 12, 22 -- X-Mailer: Apple Mail (2.752.2) 12, 22 -- X-Junkmail-Whitelist: YES (by domain whitelist at sentry.ucr.edu) 12, 22 -- Content-Transfer-Encoding: 8bit 12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5LFYhhQ030852 ==============================End of - Headers==============================
Tina's query has brought to mind a parallel question that we have been pondering.
Is the exact pH of the fix buffer important in fixing standard biological tissue (not extremophiles).
Over the years, I have followed protocols that used pH 7.6 for some tissues (vertebrates and invertebrates), and pH 7.4 or pH 7.2 for others. This was never done systematically, but mostly by indirection. We have recently been suffering some poor results on invertebrate tissues - and wondering if there is a perfect pH that would yield best results. Is it conceivable that a small shift in pH is critical? In particular we have been testing microwave protocols, where access across the outer cuticle may be limiting entry/exit of fluids into the intact animal.
Any suggestions? I have always much more about osmolarity rather than pH.
Thanks in advance,
DHH -- David H. Hall, Ph.D. Center for C. elegans Anatomy Dominic P. Purpura Department of Neuroscience Albert Einstein College of Medicine 1410 Pelham Parkway Bronx, NY 10461
www.wormatlas.org www.wormimage.org
phone 718 430-2195 fax 718 430-2514
==============================Original Headers============================== 8, 25 -- From hall-at-aecom.yu.edu Thu Jun 21 11:26:47 2007 8, 25 -- Received: from mx1.aecom.yu.edu (mx1.aecom.yu.edu [129.98.1.51]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5LGQlnY011975 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 11:26:47 -0500 8, 25 -- Received: from draco.aecom.yu.edu (draco.aecom.yu.edu [129.98.1.160]) 8, 25 -- by mx1.aecom.yu.edu (Postfix) with ESMTP id ED4169F0053 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 12:26:45 -0400 (EDT) 8, 25 -- Received: from draco.aecom.yu.edu (unknown [127.0.0.1]) 8, 25 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 729728B4003 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 12:19:13 -0400 (EDT) 8, 25 -- X-AuditID: 816201a0-9979cbb000001801-c8-467aa501b50a 8, 25 -- Received: from post.aecom.yu.edu (post.aecom.yu.edu [129.98.1.100]) 8, 25 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 3C8AB718002 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 12:19:13 -0400 (EDT) 8, 25 -- Received: from [129.98.90.160] (worm.aecom.yu.edu [129.98.90.160]) 8, 25 -- by post.aecom.yu.edu (Postfix) with ESMTP id 07D3859 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 12:26:44 -0400 (EDT) 8, 25 -- Mime-Version: 1.0 8, 25 -- Message-Id: {a06240802c2a0552fa328-at-[129.98.90.160]} 8, 25 -- Date: Thu, 21 Jun 2007 12:28:16 -0400 8, 25 -- To: microscopy-at-microscopy.com 8, 25 -- From: David Hall {hall-at-aecom.yu.edu} 8, 25 -- Subject: pH effects on fixation 8, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 8, 25 -- X-Brightmail-Tracker: AAAAAA== ==============================End of - Headers==============================
I have not done this systematically, but my impression is that the osmolarity matters and the pH is much less (if at all) important. That said, I have never done microwave, so I am not sure if that changes things.
David
_____________________
David Elliott Ph.D. Assistant Professor - Department of Cell Biology and Anatomy Director, Research Microscopy Core Service University of Arizona College of Medicine PO Box 245004 Tucson, AZ 85724
Voice: 520-626-7870 Fax: 520-626-2097
On Jun 21, 2007, at 9:29 AM, hall-at-aecom.yu.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Tina's query has brought to mind a parallel question that we have } been pondering. } } Is the exact pH of the fix buffer important in fixing standard } biological tissue (not extremophiles). } } Over the years, I have followed protocols that used pH 7.6 for some } tissues (vertebrates and invertebrates), and pH 7.4 or pH 7.2 for } others. This was never done systematically, but mostly by } indirection. We have recently been suffering some poor results on } invertebrate tissues - and wondering if there is a perfect pH that } would yield best results. Is it conceivable that a small shift in pH } is critical? In particular we have been testing microwave protocols, } where access across the outer cuticle may be limiting entry/exit of } fluids into the intact animal. } } Any suggestions? I have always much more about osmolarity rather } than pH. } } Thanks in advance, } } DHH } -- } David H. Hall, Ph.D. } Center for C. elegans Anatomy } Dominic P. Purpura Department of Neuroscience } Albert Einstein College of Medicine } 1410 Pelham Parkway } Bronx, NY 10461 } } www.wormatlas.org } www.wormimage.org } } phone 718 430-2195 } fax 718 430-2514 } } ==============================Original } Headers============================== } 8, 25 -- From hall-at-aecom.yu.edu Thu Jun 21 11:26:47 2007 } 8, 25 -- Received: from mx1.aecom.yu.edu (mx1.aecom.yu.edu } [129.98.1.51]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id l5LGQlnY011975 } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 } 11:26:47 -0500 } 8, 25 -- Received: from draco.aecom.yu.edu (draco.aecom.yu.edu } [129.98.1.160]) } 8, 25 -- by mx1.aecom.yu.edu (Postfix) with ESMTP id ED4169F0053 } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 } 12:26:45 -0400 (EDT) } 8, 25 -- Received: from draco.aecom.yu.edu (unknown [127.0.0.1]) } 8, 25 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP } id 729728B4003 } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 } 12:19:13 -0400 (EDT) } 8, 25 -- X-AuditID: 816201a0-9979cbb000001801-c8-467aa501b50a } 8, 25 -- Received: from post.aecom.yu.edu (post.aecom.yu.edu } [129.98.1.100]) } 8, 25 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP } id 3C8AB718002 } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 } 12:19:13 -0400 (EDT) } 8, 25 -- Received: from [129.98.90.160] (worm.aecom.yu.edu } [129.98.90.160]) } 8, 25 -- by post.aecom.yu.edu (Postfix) with ESMTP id 07D3859 } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 } 12:26:44 -0400 (EDT) } 8, 25 -- Mime-Version: 1.0 } 8, 25 -- Message-Id: {a06240802c2a0552fa328-at-[129.98.90.160]} } 8, 25 -- Date: Thu, 21 Jun 2007 12:28:16 -0400 } 8, 25 -- To: microscopy-at-microscopy.com } 8, 25 -- From: David Hall {hall-at-aecom.yu.edu} } 8, 25 -- Subject: pH effects on fixation } 8, 25 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } 8, 25 -- X-Brightmail-Tracker: AAAAAA== } ==============================End of - } Headers==============================
==============================Original Headers============================== 11, 22 -- From Elliott-at-arizona.edu Thu Jun 21 12:01:30 2007 11, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.132.44]) 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5LH1UrE024223 11, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 21 Jun 2007 12:01:30 -0500 11, 22 -- Received: from gandalfs_amavis (amavis1.email.arizona.edu [10.0.0.204]) 11, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 2034D549BE 11, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 21 Jun 2007 10:01:30 -0700 (MST) 11, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 11, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 842CE61C64 11, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 21 Jun 2007 10:01:25 -0700 (MST) 11, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 11, 22 -- In-Reply-To: {200706211629.l5LGT9xF016921-at-ns.microscopy.com} 11, 22 -- References: {200706211629.l5LGT9xF016921-at-ns.microscopy.com} 11, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 11, 22 -- Message-Id: {10075CB6-FE46-4131-97E1-65525D0FA0F2-at-arizona.edu} 11, 22 -- Content-Transfer-Encoding: 7bit 11, 22 -- From: David Elliott {Elliott-at-arizona.edu} 11, 22 -- Subject: Re: [Microscopy] pH effects on fixation 11, 22 -- Date: Thu, 21 Jun 2007 10:01:24 -0700 11, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 11, 22 -- X-Mailer: Apple Mail (2.752.2) 11, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
There is an optimum for everything, so there is no doubt that an optimal pH exists. The question is whether it is worth to spend much time to find it when you can be happy with a "routine" protocol.
--- Elliott-at-arizona.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi David } } I have not done this systematically, but my } impression is that the } osmolarity matters and the pH is much less (if at } all) important. } That said, I have never done microwave, so I am not } sure if that } changes things. } } David } } } _____________________ } } David Elliott Ph.D. } Assistant Professor - Department of Cell Biology and } Anatomy } Director, Research Microscopy Core Service } University of Arizona College of Medicine } PO Box 245004 } Tucson, AZ 85724 } } Voice: 520-626-7870 } Fax: 520-626-2097 } } } On Jun 21, 2007, at 9:29 AM, hall-at-aecom.yu.edu } wrote: } } } } } } } } } } ---------------------------------------------------------------------- } } } ------ } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/ } } MicroscopyListserver } } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------- } } } ------ } } } } Tina's query has brought to mind a parallel } question that we have } } been pondering. } } } } Is the exact pH of the fix buffer important in } fixing standard } } biological tissue (not extremophiles). } } } } Over the years, I have followed protocols that } used pH 7.6 for some } } tissues (vertebrates and invertebrates), and pH } 7.4 or pH 7.2 for } } others. This was never done systematically, but } mostly by } } indirection. We have recently been suffering some } poor results on } } invertebrate tissues - and wondering if there is a } perfect pH that } } would yield best results. Is it conceivable that } a small shift in pH } } is critical? In particular we have been testing } microwave protocols, } } where access across the outer cuticle may be } limiting entry/exit of } } fluids into the intact animal. } } } } Any suggestions? I have always much more about } osmolarity rather } } than pH. } } } } Thanks in advance, } } } } DHH } } -- } } David H. Hall, Ph.D. } } Center for C. elegans Anatomy } } Dominic P. Purpura Department of Neuroscience } } Albert Einstein College of Medicine } } 1410 Pelham Parkway } } Bronx, NY 10461 } } } } www.wormatlas.org } } www.wormimage.org } } } } phone 718 430-2195 } } fax 718 430-2514 } } } } ==============================Original } } Headers============================== } } 8, 25 -- From hall-at-aecom.yu.edu Thu Jun 21 } 11:26:47 2007 } } 8, 25 -- Received: from mx1.aecom.yu.edu } (mx1.aecom.yu.edu } } [129.98.1.51]) } } 8, 25 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP } } id l5LGQlnY011975 } } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 } Jun 2007 } } 11:26:47 -0500 } } 8, 25 -- Received: from draco.aecom.yu.edu } (draco.aecom.yu.edu } } [129.98.1.160]) } } 8, 25 -- by mx1.aecom.yu.edu (Postfix) with ESMTP } id ED4169F0053 } } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 } Jun 2007 } } 12:26:45 -0400 (EDT) } } 8, 25 -- Received: from draco.aecom.yu.edu } (unknown [127.0.0.1]) } } 8, 25 -- by draco.aecom.yu.edu (Symantec Mail } Security) with ESMTP } } id 729728B4003 } } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 } Jun 2007 } } 12:19:13 -0400 (EDT) } } 8, 25 -- X-AuditID: } 816201a0-9979cbb000001801-c8-467aa501b50a } } 8, 25 -- Received: from post.aecom.yu.edu } (post.aecom.yu.edu } } [129.98.1.100]) } } 8, 25 -- by draco.aecom.yu.edu (Symantec Mail } Security) with ESMTP } } id 3C8AB718002 } } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 } Jun 2007 } } 12:19:13 -0400 (EDT) } } 8, 25 -- Received: from [129.98.90.160] } (worm.aecom.yu.edu } } [129.98.90.160]) } } 8, 25 -- by post.aecom.yu.edu (Postfix) with } ESMTP id 07D3859 } } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 } Jun 2007 } } 12:26:44 -0400 (EDT) } } 8, 25 -- Mime-Version: 1.0 } } 8, 25 -- Message-Id: } {a06240802c2a0552fa328-at-[129.98.90.160]} } } 8, 25 -- Date: Thu, 21 Jun 2007 12:28:16 -0400 } } 8, 25 -- To: microscopy-at-microscopy.com } } 8, 25 -- From: David Hall {hall-at-aecom.yu.edu} } } 8, 25 -- Subject: pH effects on fixation } } 8, 25 -- Content-Type: text/plain; } charset="us-ascii" ; } } format="flowed" } } 8, 25 -- X-Brightmail-Tracker: AAAAAA== } } ==============================End of - } } Headers============================== } } } ==============================Original } Headers============================== } 11, 22 -- From Elliott-at-arizona.edu Thu Jun 21 } 12:01:30 2007 } 11, 22 -- Received: from smtpgate.email.arizona.edu } (gandalf.email.arizona.edu [128.196.132.44]) } 11, 22 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l5LH1UrE024223 } 11, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, } 21 Jun 2007 12:01:30 -0500 } 11, 22 -- Received: from gandalfs_amavis } (amavis1.email.arizona.edu [10.0.0.204]) } 11, 22 -- by smtpgate.email.arizona.edu (Postfix) } with ESMTP id 2034D549BE } 11, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, } 21 Jun 2007 10:01:30 -0700 (MST) } 11, 22 -- Received: from [150.135.145.126] (unknown } [150.135.145.126]) } 11, 22 -- by smtpgate.email.arizona.edu (Postfix) } with ESMTP id 842CE61C64 } 11, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, } 21 Jun 2007 10:01:25 -0700 (MST) } 11, 22 -- Mime-Version: 1.0 (Apple Message framework } v752.2) } 11, 22 -- In-Reply-To: } {200706211629.l5LGT9xF016921-at-ns.microscopy.com} } 11, 22 -- References: } {200706211629.l5LGT9xF016921-at-ns.microscopy.com} } === message truncated ===
____________________________________________________________________________________ Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. http://sims.yahoo.com/
==============================Original Headers============================== 7, 21 -- From nizets2-at-yahoo.com Thu Jun 21 14:59:04 2007 7, 21 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5LJx4tH009221 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 14:59:04 -0500 7, 21 -- Received: (qmail 76490 invoked by uid 60001); 21 Jun 2007 19:59:03 -0000 7, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 21 -- s=s1024; d=yahoo.com; 7, 21 -- h=X-YMail-OSG:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 21 -- b=I3/g9umZfY0n+OFRtR9SEB2MrZNqbV+Rb7oOhPO/HVrJ8yjm5D7OIe4bUJiSRHXVZPOyzGsVNnaGCW9yzYwgxxowMz91ds9wRrSPqJVwZN4wCpflr7WzFK9M2SYdb1cgtJwYK07Ipj4I9x2KdVZk7lE9VhVRurtn7tZTiRO9XIA=; 7, 21 -- X-YMail-OSG: gsiqaxgVM1lmkObnCoPUwQvJ0sSEVyqWX.qWaTt8bScen7O9f.VVc3ACKJ0o5FXTneP6fvjyxMy0Y_e2fuEwQCkPJCnj6rOlewqnxAE_.6_Cj63CXO9YDEAi9.wGU.NsZ7Df8jSlzkTS6Di4DJw2CshWVmcJM1nHGVOvi7W7vGtnxfsbbHRIJWk- 7, 21 -- Received: from [80.121.39.138] by web37405.mail.mud.yahoo.com via HTTP; Thu, 21 Jun 2007 12:59:03 PDT 7, 21 -- Date: Thu, 21 Jun 2007 12:59:03 -0700 (PDT) 7, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 21 -- Subject: Re: [Microscopy] Re: pH effects on fixation 7, 21 -- To: Elliott-at-arizona.edu 7, 21 -- Cc: microscopy-at-microscopy.com 7, 21 -- In-Reply-To: {200706211705.l5LH5jNC031844-at-ns.microscopy.com} 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: text/plain; charset=iso-8859-1 7, 21 -- Content-Transfer-Encoding: 8bit 7, 21 -- Message-ID: {853094.62933.qm-at-web37405.mail.mud.yahoo.com} ==============================End of - Headers==============================
I think that generally the pH should be "neutral" to keep proteins soluble, but that is a fairly broad range. The buffering capacity of the fixative may be important to keep the pH "good" in use; in plants, penetration of the fixatives can be slow and the vacuolar sap is acidic so if the membrane becomes permeablized before enough buffering is present the environment for the actual fixation in internal cells may go acidic during the critical minutes of early fixation. I think that some of the high pH fixes for plants (} pH 8) may be trying to hedge a bit, starting on the high edge so the result is neutral?
I wrote to Tina a couple of days ago about the extremophiles low pH and admitted to having to fix beef samples at pH 3.5 and I was surprised that the the tissue (if we can generosly call it that..please .. for my self esteem...) gelled very quickly at pH 3.5. Here it is important to have the pH low during fixation because the "ex-tissue" does wild things structurally and changing the pH to neutral could well alter the structure before it fixes. However cells growing -at- pH3.5 may well have neutral cytoplasm - maybe someone knows.
In the early 70's, a professor I worked for was excitedly showing my glutaraldehyde-fixed, plastic-embedded material to his colleague, a famous tropical botanist. After the tropical botanist was allowed some period of study, he was asked what he thought. He thoughtfully replied, "It is all very nice, but the cytoplasm obscures all the detail".
So, optimum is relative to the need and end result?
Dale Callaham The University of Massachusetts -at- Amherst
nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi! } } There is an optimum for everything, so there is no } doubt that an optimal pH exists. The question is } whether it is worth to spend much time to find it when } you can be happy with a "routine" protocol. } } --- Elliott-at-arizona.edu wrote: } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The } } Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi David } } } } I have not done this systematically, but my } } impression is that the } } osmolarity matters and the pH is much less (if at } } all) important. } } That said, I have never done microwave, so I am not } } sure if that } } changes things. } } } } David } } } } } } _____________________ } } } } David Elliott Ph.D. } } Assistant Professor - Department of Cell Biology and } } Anatomy } } Director, Research Microscopy Core Service } } University of Arizona College of Medicine } } PO Box 245004 } } Tucson, AZ 85724 } } } } Voice: 520-626-7870 } } Fax: 520-626-2097 } } } } } } On Jun 21, 2007, at 9:29 AM, hall-at-aecom.yu.edu } } wrote: } } } } } } } } } } } } ---------------------------------------------------------------------- } } } ------ } } } The Microscopy ListServer -- CoSponsor: The } } Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/ } } } MicroscopyListserver } } } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } } } ------ } } } } } } Tina's query has brought to mind a parallel } } question that we have } } } been pondering. } } } } } } Is the exact pH of the fix buffer important in } } fixing standard } } } biological tissue (not extremophiles). } } } } } } Over the years, I have followed protocols that } } used pH 7.6 for some } } } tissues (vertebrates and invertebrates), and pH } } 7.4 or pH 7.2 for } } } others. This was never done systematically, but } } mostly by } } } indirection. We have recently been suffering some } } poor results on } } } invertebrate tissues - and wondering if there is a } } perfect pH that } } } would yield best results. Is it conceivable that } } a small shift in pH } } } is critical? In particular we have been testing } } microwave protocols, } } } where access across the outer cuticle may be } } limiting entry/exit of } } } fluids into the intact animal. } } } } } } Any suggestions? I have always much more about } } osmolarity rather } } } than pH. } } } } } } Thanks in advance, } } } } } } DHH } } } -- } } } David H. Hall, Ph.D. } } } Center for C. elegans Anatomy } } } Dominic P. Purpura Department of Neuroscience } } } Albert Einstein College of Medicine } } } 1410 Pelham Parkway } } } Bronx, NY 10461 } } } } } } www.wormatlas.org } } } www.wormimage.org } } } } } } phone 718 430-2195 } } } fax 718 430-2514 } } } } } } ==============================Original } } } Headers============================== } } } 8, 25 -- From hall-at-aecom.yu.edu Thu Jun 21 } } 11:26:47 2007 } } } 8, 25 -- Received: from mx1.aecom.yu.edu } } (mx1.aecom.yu.edu } } } [129.98.1.51]) } } } 8, 25 -- by ns.microscopy.com } } (8.12.11.20060308/8.12.8) with ESMTP } } } id l5LGQlnY011975 } } } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 } } Jun 2007 } } } 11:26:47 -0500 } } } 8, 25 -- Received: from draco.aecom.yu.edu } } (draco.aecom.yu.edu } } } [129.98.1.160]) } } } 8, 25 -- by mx1.aecom.yu.edu (Postfix) with ESMTP } } id ED4169F0053 } } } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 } } Jun 2007 } } } 12:26:45 -0400 (EDT) } } } 8, 25 -- Received: from draco.aecom.yu.edu } } (unknown [127.0.0.1]) } } } 8, 25 -- by draco.aecom.yu.edu (Symantec Mail } } Security) with ESMTP } } } id 729728B4003 } } } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 } } Jun 2007 } } } 12:19:13 -0400 (EDT) } } } 8, 25 -- X-AuditID: } } 816201a0-9979cbb000001801-c8-467aa501b50a } } } 8, 25 -- Received: from post.aecom.yu.edu } } (post.aecom.yu.edu } } } [129.98.1.100]) } } } 8, 25 -- by draco.aecom.yu.edu (Symantec Mail } } Security) with ESMTP } } } id 3C8AB718002 } } } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 } } Jun 2007 } } } 12:19:13 -0400 (EDT) } } } 8, 25 -- Received: from [129.98.90.160] } } (worm.aecom.yu.edu } } } [129.98.90.160]) } } } 8, 25 -- by post.aecom.yu.edu (Postfix) with } } ESMTP id 07D3859 } } } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 21 } } Jun 2007 } } } 12:26:44 -0400 (EDT) } } } 8, 25 -- Mime-Version: 1.0 } } } 8, 25 -- Message-Id: } } {a06240802c2a0552fa328-at-[129.98.90.160]} } } } 8, 25 -- Date: Thu, 21 Jun 2007 12:28:16 -0400 } } } 8, 25 -- To: microscopy-at-microscopy.com } } } 8, 25 -- From: David Hall {hall-at-aecom.yu.edu} } } } 8, 25 -- Subject: pH effects on fixation } } } 8, 25 -- Content-Type: text/plain; } } charset="us-ascii" ; } } } format="flowed" } } } 8, 25 -- X-Brightmail-Tracker: AAAAAA== } } } ==============================End of - } } } Headers============================== } } } } ==============================Original } } Headers============================== } } 11, 22 -- From Elliott-at-arizona.edu Thu Jun 21 } } 12:01:30 2007 } } 11, 22 -- Received: from smtpgate.email.arizona.edu } } (gandalf.email.arizona.edu [128.196.132.44]) } } 11, 22 -- by ns.microscopy.com } } (8.12.11.20060308/8.12.8) with ESMTP id } } l5LH1UrE024223 } } 11, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, } } 21 Jun 2007 12:01:30 -0500 } } 11, 22 -- Received: from gandalfs_amavis } } (amavis1.email.arizona.edu [10.0.0.204]) } } 11, 22 -- by smtpgate.email.arizona.edu (Postfix) } } with ESMTP id 2034D549BE } } 11, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, } } 21 Jun 2007 10:01:30 -0700 (MST) } } 11, 22 -- Received: from [150.135.145.126] (unknown } } [150.135.145.126]) } } 11, 22 -- by smtpgate.email.arizona.edu (Postfix) } } with ESMTP id 842CE61C64 } } 11, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, } } 21 Jun 2007 10:01:25 -0700 (MST) } } 11, 22 -- Mime-Version: 1.0 (Apple Message framework } } v752.2) } } 11, 22 -- In-Reply-To: } } {200706211629.l5LGT9xF016921-at-ns.microscopy.com} } } 11, 22 -- References: } } {200706211629.l5LGT9xF016921-at-ns.microscopy.com} } } } === message truncated === } } } } } ____________________________________________________________________________________ } Moody friends. 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==============================Original Headers============================== 6, 22 -- From dac-at-research.umass.edu Thu Jun 21 15:36:35 2007 6, 22 -- Received: from race6.oit.umass.edu (race6.oit.umass.edu [128.119.101.44]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5LKaXMH021838 6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 15:36:34 -0500 6, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 6, 22 -- (authenticated bits=0) 6, 22 -- by race6.oit.umass.edu (8.13.7/8.13.7) with ESMTP id l5LKaSwb017685 6, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 16:36:29 -0400 6, 22 -- Message-ID: {467AEFFD.8070404-at-research.umass.edu} 6, 22 -- Date: Thu, 21 Jun 2007 16:39:09 -0500 6, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 6, 22 -- Reply-To: dac-at-research.umass.edu 6, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.4) Gecko/20070509 SeaMonkey/1.1.2 6, 22 -- MIME-Version: 1.0 6, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 22 -- Subject: Re: [Microscopy] pH effects on fixation 6, 22 -- References: {200706212008.l5LK8tet019174-at-ns.microscopy.com} 6, 22 -- In-Reply-To: {200706212008.l5LK8tet019174-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
We are upgrading our microscopes and have some surplus
1)Jeol JSM-35C Working SEM, has been used as digital scope with A/D card (not included). Includes Link Analytical backscattering detector.
2)Zeiss EM-10 Working TEM, fantastic resolution, includes Scion digital camera
3)ISI-40 SEM, working condition
4)Jeol 100-S TEM, lots of work done to restore machine, but has not been turned on recently
--
¦ Anatoli Oleynik, Research Associate - BioWarn, LLC. ¦ 964 East St. Suite 106a - Pittsboro, NC 27312 ¦ O: 919.542.7410 | M: 919.260.1911 | F: 919.542.0268 ¦ mobile email/MSN-M: cell.toli-at-hotmail.com ¦ www.biowarnllc.com
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==============================Original Headers============================== 11, 23 -- From toli-at-hillweb.com Thu Jun 21 19:03:18 2007 11, 23 -- Received: from imf16aec.mail.bellsouth.net (imf16aec.mail.bellsouth.net [205.152.59.64]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5M03IpI005006 11, 23 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 19:03:18 -0500 11, 23 -- Received: from ibm56aec.bellsouth.net ([68.221.62.63]) 11, 23 -- by imf16aec.mail.bellsouth.net with ESMTP 11, 23 -- id {20070622000318.PSQE29796.imf16aec.mail.bellsouth.net-at-ibm56aec.bellsouth.net} 11, 23 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 20:03:18 -0400 11, 23 -- Received: from [192.168.2.4] (really [68.221.62.63]) 11, 23 -- by ibm56aec.bellsouth.net with ESMTP 11, 23 -- id {20070622000317.LVPI7706.ibm56aec.bellsouth.net-at-[192.168.2.4]} 11, 23 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jun 2007 20:03:17 -0400 11, 23 -- Message-ID: {467B11C6.1090209-at-hillweb.com} 11, 23 -- Disposition-Notification-To: Anatoli Oleynik {toli-at-hillweb.com} 11, 23 -- Date: Thu, 21 Jun 2007 20:03:18 -0400 11, 23 -- From: Anatoli Oleynik {toli-at-hillweb.com} 11, 23 -- Organization: BioWarn LLC 11, 23 -- User-Agent: Thunderbird 2.0.0.4 (Windows/20070604) 11, 23 -- MIME-Version: 1.0 11, 23 -- To: microscopy-at-microscopy.com 11, 23 -- Subject: [Microscopy] microscopes avaliable 11, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 23 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Title-Subject: [Filtered] electron microscopy immunohistochemistry
Question: Non-specific labelling of myelin in epoxy resin embedded brain tissue!!!!! Post-embedding immunocytochemistry of semi-thin (one micron thick) animal brain sections using ABC Technique, results in very high non-specific labelling of myelin. I have tried applying every blocking reagent I know with no success.
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Email: toli-at-hillweb.com Name: Anatoli Oleynik
Organization: BioWarn
Title-Subject: [Filtered] Anyone have a good home?
Question: Electron microscopes available: {br} 1)Jeol JSM-35C Working SEM, has been used as digital scope with A/D card (not included). Includes Link Analytical backscattering detector. {br} 2)Zeiss EM-10 Working TEM, fantastic resolution, includes Scion digital camera {br} 3)ISI-40 SEM, working condition {br} 4)Jeol 100-S TEM, lots of work done to restore machine, but has not been turned on recently {br}
Looking for a generic method for labeling DNA such that it shows up in the SEM. Something genric like DAPI for LM rather than an insitu hybridization with a gold label.
Any suggestions?
Some old Silver staining technique?
Thanks Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
==============================Original Headers============================== 6, 22 -- From edelmare-at-muohio.edu Fri Jun 22 12:54:30 2007 6, 22 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5MHsUX3017918 6, 22 -- for {microscopy-at-Microscopy.com} ; Fri, 22 Jun 2007 12:54:30 -0500 6, 22 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 6, 22 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l5MHsUHe001066 6, 22 -- for {microscopy-at-Microscopy.com} ; Fri, 22 Jun 2007 13:54:30 -0400 6, 22 -- Received: from [192.168.1.23] ([134.53.14.105]) 6, 22 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l5MHsTEw001416 6, 22 -- for {microscopy-at-Microscopy.com} ; Fri, 22 Jun 2007 13:54:29 -0400 6, 22 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 6, 22 -- To: microscopy-at-Microscopy.com 6, 22 -- Date: Fri, 22 Jun 2007 13:54:26 -0400 6, 22 -- MIME-Version: 1.0 6, 22 -- Subject: Identify DNA in SEM 6, 22 -- Message-ID: {467BD492.7288.2390CD68-at-edelmare.muohio.edu} 6, 22 -- Priority: normal 6, 22 -- X-mailer: Pegasus Mail for Windows (4.41) 6, 22 -- Content-type: text/plain; charset=US-ASCII 6, 22 -- Content-transfer-encoding: 7BIT 6, 22 -- Content-description: Mail message body 6, 22 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
How about an anti-DNA antibody? Been a while since I've used one, that was for immunoblots, not EM. Most immunological suppliers can sell you one. You can then detect with a gold labelled secondary antibody.
Paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-3926
==============================Original Headers============================== 6, 21 -- From paul_hazelton-at-umanitoba.ca Fri Jun 22 13:06:00 2007 6, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5MI5w8Q029579 6, 21 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jun 2007 13:05:59 -0500 6, 21 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 6, 21 -- (authenticated bits=0) 6, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id l5MI5vI1000203; 6, 21 -- Fri, 22 Jun 2007 13:05:58 -0500 (CDT) 6, 21 -- Message-ID: {467C0F09.3000202-at-umanitoba.ca} 6, 21 -- Date: Fri, 22 Jun 2007 13:03:53 -0500 6, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 6, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 6, 21 -- X-Accept-Language: en-us, en 6, 21 -- MIME-Version: 1.0 6, 21 -- To: edelmare-at-muohio.edu 6, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com} 6, 21 -- Subject: Re: [Microscopy] Identify DNA in SEM 6, 21 -- References: {200706221756.l5MHur9Q021229-at-ns.microscopy.com} 6, 21 -- In-Reply-To: {200706221756.l5MHur9Q021229-at-ns.microscopy.com} 6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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==============================Original Headers============================== 3, 25 -- From mnesta-at-ebsciences.com Fri Jun 22 14:14:00 2007 3, 25 -- Received: from mx-outbound01.easydns.com (mailout.easydns.com [205.210.42.54]) 3, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5MJE0Fh010445 3, 25 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jun 2007 14:14:00 -0500 3, 25 -- Received: from mail.ebsciences.com (unknown [69.182.224.2]) 3, 25 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 3, 25 -- (No client certificate requested) 3, 25 -- by mx-outbound01.easydns.com (Postfix) with ESMTP id 54DAE8056 3, 25 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jun 2007 15:13:59 -0400 (EDT) 3, 25 -- Received: from mnesta.ebsciences.private ([10.10.0.153]) 3, 25 -- by mail.ebsciences.com with esmtpsa (TLSv1:AES256-SHA:256) 3, 25 -- (Exim 4.67) 3, 25 -- (envelope-from {mnesta-at-ebsciences.com} ) 3, 25 -- id 1I1oZz-0002fr-ID 3, 25 -- for microscopy-at-microscopy.com; Fri, 22 Jun 2007 15:13:59 -0400 3, 25 -- Message-ID: {467C1F76.6030002-at-ebsciences.com} 3, 25 -- Date: Fri, 22 Jun 2007 15:13:58 -0400 3, 25 -- From: Mike Nesta {mnesta-at-ebsciences.com} 3, 25 -- Organization: Energy Beam Sciences 3, 25 -- User-Agent: Thunderbird 1.5.0.12 (Windows/20070509) 3, 25 -- MIME-Version: 1.0 3, 25 -- To: microscopy-at-microscopy.com 3, 25 -- Subject: Unsubscribe 3, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed 3, 25 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
If it is possible, just one crazy idea: label your DNA with BrdU and detect Br by EDX. In situ, you can also use BrdU with the Tdt technique. BrdU can also be detected with very good specific antibodies.
Stephane
--- edelmare-at-muohio.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Looking for a generic method for labeling DNA such } that it shows up } in the SEM. Something genric like DAPI for LM } rather than an insitu } hybridization with a gold label. } } Any suggestions? } } Some old Silver staining technique? } } Thanks } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Director } 364 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } http://www.emf.muohio.edu } } "RAM disk is NOT an installation procedure." } } } ==============================Original } Headers============================== } 6, 22 -- From edelmare-at-muohio.edu Fri Jun 22 } 12:54:30 2007 } 6, 22 -- Received: from mulnx12.mcs.muohio.edu } (mulnx12.mcs.muohio.edu [134.53.6.67]) } 6, 22 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l5MHsUX3017918 } 6, 22 -- for {microscopy-at-Microscopy.com} ; Fri, 22 } Jun 2007 12:54:30 -0500 } 6, 22 -- Received: from mulnx23.mcs.muohio.edu } (mulnx23.mcs.muohio.edu [134.53.6.10]) } 6, 22 -- by mulnx12.mcs.muohio.edu } (Switch-3.1.8/Switch-3.1.7) with ESMTP id } l5MHsUHe001066 } 6, 22 -- for {microscopy-at-Microscopy.com} ; Fri, 22 } Jun 2007 13:54:30 -0400 } 6, 22 -- Received: from [192.168.1.23] } ([134.53.14.105]) } 6, 22 -- by mulnx23.mcs.muohio.edu } (Switch-3.1.8/Switch-3.1.7) with ESMTP id } l5MHsTEw001416 } 6, 22 -- for {microscopy-at-Microscopy.com} ; Fri, 22 } Jun 2007 13:54:29 -0400 } 6, 22 -- From: "Richard E. Edelmann" } {edelmare-at-muohio.edu} } 6, 22 -- To: microscopy-at-Microscopy.com } 6, 22 -- Date: Fri, 22 Jun 2007 13:54:26 -0400 } 6, 22 -- MIME-Version: 1.0 } 6, 22 -- Subject: Identify DNA in SEM } 6, 22 -- Message-ID: } {467BD492.7288.2390CD68-at-edelmare.muohio.edu} } 6, 22 -- Priority: normal } 6, 22 -- X-mailer: Pegasus Mail for Windows (4.41) } 6, 22 -- Content-type: text/plain; charset=US-ASCII } 6, 22 -- Content-transfer-encoding: 7BIT } 6, 22 -- Content-description: Mail message body } 6, 22 -- X-Scanned-By: MIMEDefang 2.57 on } 134.53.6.67 } ==============================End of - } Headers============================== }
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==============================Original Headers============================== 8, 21 -- From nizets2-at-yahoo.com Fri Jun 22 14:21:05 2007 8, 21 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5MJL41f022038 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jun 2007 14:21:04 -0500 8, 21 -- Received: (qmail 92721 invoked by uid 60001); 22 Jun 2007 19:21:03 -0000 8, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 8, 21 -- s=s1024; d=yahoo.com; 8, 21 -- h=X-YMail-OSG:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 8, 21 -- b=OJZvL9ozc8xqfR7vrC/fVxuy+VfqEsmJbdPdDcVS5N3XtRzv2JokBCz9xQ4znUNzRogTAG9xZcKI6RDtRmozyE5yYM4dGDrRiKeFX6qLGJ0HV0NyWjhHqDKtK3YKsAvDC8hQoKh6EAEwWFP3HtreJLEUBxj1PfCFAUoUevHCd9o=; 8, 21 -- X-YMail-OSG: YHTTkVUVM1nKXDw55RRaCv65HxlGxk5PJDnfWOUcYpQ.hQXAGLBT0id8jKRhLB51noI8DAMdd1HkqyF0N0eR5Ofrmc4NwDPPtQl3oQkA.6jEI4MeFr0g.oPx_YfKIASjjcJYRdYOa_ab0yUoHvCYBQKwfqhbQSc2tfU_uGPeg5L1H2xeug4BV5A- 8, 21 -- Received: from [80.121.48.59] by web37406.mail.mud.yahoo.com via HTTP; Fri, 22 Jun 2007 12:21:03 PDT 8, 21 -- Date: Fri, 22 Jun 2007 12:21:03 -0700 (PDT) 8, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 8, 21 -- Subject: Re: [Microscopy] Identify DNA in SEM 8, 21 -- To: edelmare-at-muohio.edu 8, 21 -- Cc: microscopy-at-microscopy.com 8, 21 -- In-Reply-To: {200706221759.l5MHxlvC025504-at-ns.microscopy.com} 8, 21 -- MIME-Version: 1.0 8, 21 -- Content-Type: text/plain; charset=iso-8859-1 8, 21 -- Content-Transfer-Encoding: 8bit 8, 21 -- Message-ID: {530113.92052.qm-at-web37406.mail.mud.yahoo.com} ==============================End of - Headers==============================
I have a friend looking for a Zeiss 109, 900 or 902 TEM. If anyone is selling or giving away their old scope please let me know. I saw the posting about the EM 10 (a great scope by the way).
Thanks for any leads, Beth
********************************************************************** Beth Richardson Electron Microscopy Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
http://www.plantbio.uga.edu/emlab/
Phone - (706) 542-1790 & FAX - (706) 542-1805
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
************************************************************************ *** The Friends of the Marine Institute - Join Today! www.friendsofugami.org
==============================Original Headers============================== 11, 19 -- From beth-at-plantbio.uga.edu Fri Jun 22 16:29:12 2007 11, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5MLTCbG005673 11, 19 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jun 2007 16:29:12 -0500 11, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 11, 19 -- (authenticated user beth-at-plantbio.uga.edu) 11, 19 -- by dogwood.plantbio.uga.edu 11, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 11, 19 -- for microscopy-at-microscopy.com; 11, 19 -- Fri, 22 Jun 2007 17:29:07 -0400 11, 19 -- Mime-Version: 1.0 (Apple Message framework v752.3) 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- Message-Id: {9574CCEB-9690-45B1-8116-BA05FEC8B21E-at-plantbio.uga.edu} 11, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 11, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 11, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 11, 19 -- Subject: Zeiss TEMs 11, 19 -- Date: Fri, 22 Jun 2007 17:29:11 -0400 11, 19 -- X-Mailer: Apple Mail (2.752.3) ==============================End of - Headers==============================
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Email: tchallman-at-case4n6.com Name: T. Challman
Organization: CASE Forensics Corp
Title-Subject: [Filtered] SEM Parts Available_Phillips S505
Question: We have a Phillips S505 SEM if anyone needs parts with a Tracor Northern EDS system. Please contact me off-line if you're interested. The tool is currently in Denver, CO.
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Email: BTWells-at-petrog.co.uk Name: Barrie Wells
Organization: Conwy Valley Systems Limited
Title-Subject: [Filtered] Unanswered questions
Question: Is there a list of unanswered questions? I donít mean in life generally, just on this list. Some questions generate a wave of correspondence, others apparently none. Two questions recently were of interest to me, in that I wanted to know the answer, not that I could offer one, and so I kept an eye open, but saw nothing. The two questions were: Title-Subject: [Filtered] RE: Segmentation of particles in Gatan Digital Micrograph Software (20/06/2007) and [Microscopy] auto particle ident (30/04/2007). Did the posters receive answers off-line, or are there no answers, or were the questions too trivial for the people on the list, or are they the type of question one would expect to pay a consultant to answer? I am intrigued, especially as listers seem to give of their time and knowledge so freely on so many topics. Thanks for any insights, Barrie
Barrie, raises a valid query, and there is no such list of questions still waiting for answers. At the same time I believe there is a great number of off-line answers to questions that have been received, but are not circulated.
I'll take this opportunity to remind all subscribers that when you receive off-line answers to a question, it would be very appropriate to collect all of these into a single message (removing all respondant's names & affiliations) and then posting a SUMMARY to the listserver. In this way everyone will benefit and the answers which then also become a resource in the Listserver Archives.
There are a number of occassions where individuals do post summaries, but I expect that this percentage is on the low side relative to the actual number of responses.
Of course, if a respondant request you keep his/her reply private then by all means you should honor that request.
Cheers,
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 7, 11 -- From zaluzec-at-microscopy.com Sat Jun 23 09:07:02 2007 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5NE6x84023484 7, 11 -- for {microscopy-at-microscopy.com} ; Sat, 23 Jun 2007 09:07:02 -0500 7, 11 -- Mime-Version: 1.0 7, 11 -- Message-Id: {p06240803c2a2d7aaf42a-at-[206.69.208.22]} 7, 11 -- Date: Sat, 23 Jun 2007 09:06:59 -0500 7, 11 -- To: microscopy-at-microscopy.com 7, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 7, 11 -- Subject: Administrivia: Unanswered questions 7, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
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Email: jennings.michael-at-gmail.com Name: Mike
Organization: university of Otago
Title-Subject: [Filtered] Ruthenium Stains
Question: Hello All!
I have a quick question about contrast using Ruthenium Red stain on cells for TEM. I have some sections that have RR on them, and am wondering about the contrast for looking at microtubule structures compared to say Uranyl Acetate or OsO4?
Not quite information. Was the ruthenium red added as part of the fixation protocol (in conjunction with the glut and OsO4) or is it an en bloc stain or on the section? If it was in the initial fixative, the penetration is very slight such that probably less than a micron or so is stained (that is, on minced tissue). In intact tissues or cultured cells, the RR is confined to the cell surface. There is a whole literature on this in the 1960s and 1970s (and maybe into the 1980s--I don't remember) looking at staining with RR, alcian blue and others. I do not know of techniques using RR for either en bloc or thin section stains. Ruthenium tetroxide is a different matter--and there is a separate literature on that as well. Try searching with Google, or better, search through PubMed.
Roger Moretz, Ph.D. Dept of Toxicology Boehringer Ingelheim Pharmaceuticals, Inc. Ridgefield, CT
July 4th--true independence day--is only 8 days away!!!!!!!!!! Then I'm on the "codger side".
On 6/25/07, jennings.michael-at-gmail.com {jennings.michael-at-gmail.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both jennings.michael-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: jennings.michael-at-gmail.com } Name: Mike } } Organization: university of Otago } } Title-Subject: [Filtered] Ruthenium Stains } } Question: Hello All! } } I have a quick question about contrast using Ruthenium Red stain on cells for TEM. } I have some sections that have RR on them, and am wondering about the contrast for looking at microtubule structures compared to say Uranyl Acetate or OsO4? } } Best Regards, } } Mike } } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 11 -- From zaluzec-at-microscopy.com Mon Jun 25 08:03:48 2007 } 11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5PD3lET029966 } 11, 11 -- for {microscopy-at-microscopy.com} ; Mon, 25 Jun 2007 08:03:48 -0500 } 11, 11 -- Mime-Version: 1.0 } 11, 11 -- Message-Id: {p06240800c2a56d9f1c86-at-[206.69.208.22]} } 11, 11 -- Date: Mon, 25 Jun 2007 08:03:45 -0500 } 11, 11 -- To: microscopy-at-microscopy.com } 11, 11 -- From: jennings.michael-at-gmail.com (by way of MicroscopyListserver) } 11, 11 -- Subject: viaWWW: Ruthenium Stains } 11, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 29 -- From rcmoretz-at-gmail.com Mon Jun 25 13:47:14 2007 6, 29 -- Received: from py-out-1112.google.com (py-out-1112.google.com [64.233.166.182]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5PIlBj7018435 6, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Jun 2007 13:47:13 -0500 6, 29 -- Received: by py-out-1112.google.com with SMTP id f47so2139276pye 6, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Jun 2007 11:47:09 -0700 (PDT) 6, 29 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 6, 29 -- d=gmail.com; s=beta; 6, 29 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 6, 29 -- b=snL/RbA4JZZkBmkow0DuIWiLhBRu4Z0denj1iD5+VcVPgpPVj3FT80jyzD80t02OcH8VjLARFJvt/zeNVYaXxgqv8+3c4hMane8wWhZsY8ClB2zpPvGdiD4vzazZ/jzeFKtQB3YO2vvk9J7rpFRHoT2NrsLjELMC0tKCexbO1w4= 6, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 29 -- d=gmail.com; s=beta; 6, 29 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 6, 29 -- b=PB7CPNrmK4tC14fL76Ysj+F4H29ojOgaKxmBwQGv6+OIqBwbvfbuZ+KPeDRhCa0TeKO8clrHZgADgIN3vyh73+pJD8Xc7jRi7JojFydY1x/EilOHQ6tt/n9O2v7+WD3t5gMiiZt4OdKQMwtIzPXUBd/Vup6Wm2ul1ajg6yXTgNg= 6, 29 -- Received: by 10.65.132.13 with SMTP id j13mr9741976qbn.1182797229386; 6, 29 -- Mon, 25 Jun 2007 11:47:09 -0700 (PDT) 6, 29 -- Received: by 10.64.185.3 with HTTP; Mon, 25 Jun 2007 11:47:09 -0700 (PDT) 6, 29 -- Message-ID: {950e3cfd0706251147r1582e67dy8b41f00bca7f7489-at-mail.gmail.com} 6, 29 -- Date: Mon, 25 Jun 2007 14:47:09 -0400 6, 29 -- From: "Roger Moretz" {rcmoretz-at-gmail.com} 6, 29 -- To: jennings.michael-at-gmail.com, 6, 29 -- "Microscopy Listserv" {Microscopy-at-microscopy.com} 6, 29 -- Subject: Re: [Microscopy] viaWWW: Ruthenium Stains 6, 29 -- In-Reply-To: {200706251313.l5PDDKkg005592-at-ns.microscopy.com} 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 29 -- Content-Transfer-Encoding: 7bit 6, 29 -- Content-Disposition: inline 6, 29 -- References: {200706251313.l5PDDKkg005592-at-ns.microscopy.com} ==============================End of - Headers==============================
Ok. So I left the word "enough" out of the 1st sentence. I'm ready to retire. Who cares, right? Then there were the many many many many many "out of office" replies. Give me a break.
Roger Moretz
==============================Original Headers============================== 2, 26 -- From rcmoretz-at-gmail.com Mon Jun 25 14:23:17 2007 2, 26 -- Received: from py-out-1112.google.com (py-out-1112.google.com [64.233.166.178]) 2, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5PJNFIi030620 2, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Jun 2007 14:23:16 -0500 2, 26 -- Received: by py-out-1112.google.com with SMTP id f47so2160806pye 2, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Jun 2007 12:23:14 -0700 (PDT) 2, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 2, 26 -- d=gmail.com; s=beta; 2, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 2, 26 -- b=b4ciAI2oxoaPLM2Obb/YeJeN55z2yHJaLzVvFfO3SUEKCLMyN4OQBsnt10xtxV7KhkmpiS5rQTMyD+fqytwmRbV7JeDkbJfOjoFl5VwkhwEx0y1t62EXmbreczsO+vLITbQtse7Q8SogLezwgk8v6hFI1tpMisTDRCY2oeAVVTQ= 2, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 2, 26 -- d=gmail.com; s=beta; 2, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 2, 26 -- b=Muw8gu9Q2uzATUuB3d7/l5YhBdcFh0VxoKpSguUkNAaFV+RO9pcjQ7RAEboRpV6tu/li8W+eMzQc8obz27bd6QqnQYXQDH3WjZeD63SAgw1Mv24wtJ7RjN9nTFY8EyoR+mWrjWynzyILm/TmvFpmLxlZekazSJqiL/rOW82I3t8= 2, 26 -- Received: by 10.65.153.10 with SMTP id f10mr9801093qbo.1182799393814; 2, 26 -- Mon, 25 Jun 2007 12:23:13 -0700 (PDT) 2, 26 -- Received: by 10.64.185.3 with HTTP; Mon, 25 Jun 2007 12:23:13 -0700 (PDT) 2, 26 -- Message-ID: {950e3cfd0706251223w2dfe2786gd18aa012ed9f2cbe-at-mail.gmail.com} 2, 26 -- Date: Mon, 25 Jun 2007 15:23:13 -0400 2, 26 -- From: "Roger Moretz" {rcmoretz-at-gmail.com} 2, 26 -- To: "Microscopy Listserv" {Microscopy-at-microscopy.com} 2, 26 -- Subject: Re: ruthenium red 2, 26 -- MIME-Version: 1.0 2, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 2, 26 -- Content-Transfer-Encoding: 7bit 2, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
This is not such an easy request since most generic EM stains will disclose both DNA and RNA. In the SEM, however, if the RNA is diffused and not concentrated (as the DNA in chromosomes), then UA may suffice.
Ammoniacal silver has been used to disclose nuclei in tissues examined in the SEM (Horiguchi, T, F. Sasaki, H. Takahama. 1984. Stain Technology 59:143-48).
Although I have not used it, thallium ethylate is supposed to be selective for "DNA-containing structures" (Moyne, G. 1973. Feulgen derived techniques for electron microscopical cytochemistry of DNA. J. Ultrastruc. Res. 45: 102-114). Thallium, of course, is toxic so exercise precaution. The technique is very laborious and one has to prepare the reagent using thallium metal in ethanol (10-20 days to generate). Obviously, check the reference before jumping onto this one. However, it is based on the Feulgen procedure which is rather specific, at least on the LM level, for DNA.
Good luck.
JB
} Looking for a generic method for labeling DNA such that it shows up } in the SEM. Something genric like DAPI for LM rather than an insitu } hybridization with a gold label. } } Any suggestions? } } Some old Silver staining technique? } } Thanks } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Director } 364 Pearson Hall } Miami University, Oxford, OH 45056
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
We have a Gatan Cold Stage with Control Unit Power Supply and cryo-transfer station that we bought for our Philips 410 and barely used. We are replacing the 410 with a new TEM and would like to find a home for this cryostage. As far as I know it will fit the Philips 400 and CM series, as well as some of the FEI TEMs. Can anyone suggest a mechanism for selling such an item?
Thanks!
Doug
Douglas R. Keene Assistant Investigator Micro-Imaging Center Shriners Hospital for Children 3102 S.W. Sam Jackson Park Road Portland, Oregon 97239 503-221-3434 drk-at-shcc.org
==============================Original Headers============================== 6, 22 -- From drk-at-SHCC.org Mon Jun 25 18:20:07 2007 6, 22 -- Received: from mail.SHCC.org (mail.shcc.org [64.213.211.202]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5PNK6Go026083 6, 22 -- for {Microscopy-at-Microscopy.Com} ; Mon, 25 Jun 2007 18:20:07 -0500 6, 22 -- Received: from DRK2 (drk2.research.shcc.org [64.213.211.62]) 6, 22 -- by mail.SHCC.org (PMDF V6.3 #31473) 6, 22 -- with ESMTPA id {0JK6003F8VGXXU-at-mail.SHCC.org} for Microscopy-at-Microscopy.Com; 6, 22 -- Mon, 25 Jun 2007 04:19:46 -0700 (PDT) 6, 22 -- Date: Mon, 25 Jun 2007 16:22:19 -0700 6, 22 -- From: Doug Keene {drk-at-SHCC.org} 6, 22 -- Subject: Gatan Cold Stage for Philips TEM 6, 22 -- Sender: drk-at-SHCC.org 6, 22 -- To: Microscopy-at-Microscopy.Com 6, 22 -- Reply-to: drk-at-SHCC.org 6, 22 -- Message-id: {0JK6003F9VGXXU-at-mail.SHCC.org} 6, 22 -- Organization: Shriners Hospitals for Children 6, 22 -- MIME-version: 1.0 6, 22 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 6, 22 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 6, 22 -- Content-type: text/plain; charset=us-ascii 6, 22 -- Content-transfer-encoding: 7BIT 6, 22 -- Thread-Index: Ace3f6z74z1xBci7SJqJYukVyIDx1A== ==============================End of - Headers==============================
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Title-Subject: [Filtered] TEM Engineer Position Available
Question: This position will be dedicated to TEM operation and data reporting. The person will be engaged in root cause investigation on manufacturing excursion and on defect generation for fab yield improvement, and in driving to cut down analysis TAT.
Responsibilities include: ïManage daily TEM Microscope imaging ïPerform TEM imaging and summary write ups ïOversee TEM Sample prep and provide feedback ïWork with unit process and product technology engineers to resolve device and in line issues
Qualified candidates will possess: ïMS or Ph.D. in EE, ChemE, Physics, or Material Science or equivalent ïTEM microscope hands on operation ïKnowledge of DRAM device technology and fabrication process ïFLASH device knowledge ïPermanent Residency of the United States is required.
Barrie's recent post noted that there was little public reply to my inquiry about automated particle analysis back in April, and I just received another email asking what I had learned from my call for opinions.
Therefore, due to popular demand, I'll summarize some of the replies and what I learned here:
First of all, I should explain that my software experience in this area is limited to NIH Image/ImageJ and an older version of ImageSoft's analySIS. I've been satisfied with both for analyzing a small number of images, but my collaborator was looking to analyze a large number of images automatically after she set the parameters.
My collaborator is already using a piece of software to recognize/ analyze particles in her samples, but we were interested in exploring other options. The program that she is using is called Colormod, about which I know almost nothing -- I'll be seeing some of her processed images soon, and I can offer an informed opinion then. Any listers who have used Colormod, can you send me your impressions, good or bad?
I received one message from a company that specializes in optical and non-contact metrology. They sell several different packages: Nikon Elements, Clemex, OSIS analysis (formerly SIS), and I Solutions from IMT. Their company rep thought that the "I Solution" package would probably suite the problem I described. These packages start at $900 and exceed $5000 for more advanced plug-ins. He thought the $900 package would suffice for manual interactive measurements and the $3000 package would be ideal for automatic particle shape analysis and measurement. He was nice enough to analyze one of our typical images and prepare a sample report for our review.
Someone else pointed me toward Fovea Pro, a set of plugins used with Photoshop. John Russ is the author of "The Image Processing Handbook," and his son owns Reindeer Graphics, which makes Fovea Pro, and works with his father to keep improving the software. This researcher stated that "if you are familiar with John's expertise in this field, there is noting that [Fovea Pro] cannot do, including automated classifications." He argued the advantages of Fovea Pro include integration with Photoshop, no proprietary restrictions in image formats, and the ~$800 pricetag. We have not, though, had a demo of Fovea Pro using a typical image from our study, as we had for the I Solution software.
A few other emails gradually trickled in and offered either similar or less suitable/practical options.
For now, at least, my collaborator is still working with Colormod, but I am expecting to recommend some other program, like the various packages mentioned by those who replied to my original post. I still welcome other opinions regarding this topic and what software does or does not work well for (largely) automated particle analysis.
Cheers, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu Personal Website: http://umn.edu/~frah0010
==============================Original Headers============================== 12, 23 -- From frah0010-at-umn.edu Mon Jun 25 20:47:57 2007 12, 23 -- Received: from mta-m2.tc.umn.edu (mta-m2.tc.umn.edu [134.84.119.106]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5Q1luH6019318 12, 23 -- for {microscopy-at-microscopy.com} ; Mon, 25 Jun 2007 20:47:57 -0500 12, 23 -- Received: from [10.0.1.3] (c-75-72-182-252.hsd1.mn.comcast.net [75.72.182.252]) 12, 23 -- by mta-m2.tc.umn.edu (UMN smtpd) with ESMTP 12, 23 -- Mon, 25 Jun 2007 20:47:52 -0500 (CDT) 12, 23 -- X-Umn-Remote-Mta: [N] c-75-72-182-252.hsd1.mn.comcast.net [75.72.182.252] #+TS+AU+HN 12, 23 -- In-Reply-To: {9778ed6e0706251724hd0d9a23of50aa8ce9f1ff50d-at-mail.gmail.com} 12, 23 -- References: {9778ed6e0706251724hd0d9a23of50aa8ce9f1ff50d-at-mail.gmail.com} 12, 23 -- Mime-Version: 1.0 (Apple Message framework v752.2) 12, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 12, 23 -- Message-Id: {1F4BFD9B-B845-40D3-9536-DFA51D29A075-at-umn.edu} 12, 23 -- Cc: Christine Broadbridge {broadbridgc1-at-southernct.edu} , 12, 23 -- Thomas Sadowski {thomas.sadowski-at-gmail.com} , 12, 23 -- Professor Daponte {dapontej1-at-southernct.edu} , 12, 23 -- Paidemwoyo Munhutu {munhutup1-at-gmail.com} , BTWells-at-petrog.co.uk 12, 23 -- Content-Transfer-Encoding: 7bit 12, 23 -- From: Ellery Frahm {frah0010-at-umn.edu} 12, 23 -- Subject: Re: Particle Analysis 12, 23 -- Date: Mon, 25 Jun 2007 20:47:49 -0500 12, 23 -- To: microscopy-at-microscopy.com 12, 23 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
I have enjoyed reading the discussion over the past few days and hope you don't mind if I ask a question related to the whole 200 kV versus lower kV instrument discussion. I recently had discussion with a colleague concerning specimen damage in a TEM at 200 kV versus lower kV and told them that 200 kv machines actually do less damage to samples due to faster moving electrons and therefore lower interaction times (something someone echoed here on the list). What we couldn't get to the bottom of was the reason that, when investigating carbon nanotubes, the person in question said she saw more damage in a 200 kV machine that at lower kV, suggesting there was less time to image the tubes at higher kV. My question is this, is there something particular to carbon nanotubes in the TEM, that makes their radiation damage behaviour contrary to the established rules?
Many thanks
Chris
Dr Christopher DJ Parmenter Fellow of the Royal Microscopal Society Electron Microscopy and Imaging Suite Biological Sciences University of Warwick 02476574544 christopher.parmenter-at-warwick.ac.uk
==============================Original Headers============================== 7, 33 -- From christopher.parmenter-at-warwick.ac.uk Tue Jun 26 05:04:41 2007 7, 33 -- Received: from mail-relay-1.csv.warwick.ac.uk (mail-relay-1.warwick.ac.uk [137.205.128.7]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5QA4eld007975 7, 33 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 05:04:41 -0500 7, 33 -- Received: from localhost (localhost [127.0.0.1]) 7, 33 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id l5QA4SBM069158 7, 33 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 11:04:28 +0100 (BST) 7, 33 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 7, 33 -- Received: from mail-relay-1.csv.warwick.ac.uk ([127.0.0.1]) 7, 33 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 7, 33 -- with LMTP id U4xXrF5pyyvM for {microscopy-at-microscopy.com} ; 7, 33 -- Tue, 26 Jun 2007 11:04:24 +0100 (BST) 7, 33 -- Received: from Parmenter1 (pc-ls-c008 [137.205.152.121]) 7, 33 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with SMTP id l5QA16hX067550 7, 33 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 11:01:07 +0100 (BST) 7, 33 -- X-Envelope-From: christopher.parmenter-at-warwick.ac.uk 7, 33 -- Message-ID: {00ca01c7b7d8$e632b410$7998cd89-at-Parmenter1} 7, 33 -- Reply-To: "Chris Parmenter" {christopher.parmenter-at-warwick.ac.uk} 7, 33 -- From: "Chris Parmenter" {christopher.parmenter-at-warwick.ac.uk} 7, 33 -- To: {microscopy-at-microscopy.com} 7, 33 -- Subject: Explanation of CNT behaviour in TEMs 7, 33 -- Date: Tue, 26 Jun 2007 11:00:58 +0100 7, 33 -- Organization: The University of Warwick 7, 33 -- MIME-Version: 1.0 7, 33 -- Content-Type: text/plain; 7, 33 -- format=flowed; 7, 33 -- charset="iso-8859-1"; 7, 33 -- reply-type=original 7, 33 -- Content-Transfer-Encoding: 7bit 7, 33 -- X-Priority: 3 7, 33 -- X-MSMail-Priority: Normal 7, 33 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 7, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 ==============================End of - Headers==============================
There are (at least) three mechanisms of specimen damage in the EM. In order of their occurance with kV:
1.) Radiolysis - the energy of the electrons break the molecular bonds in materials and disrupts the structure. This is the principle effect in organic materials and what most people were talking about previously. The cross section for this effect decreases with increasing Energy. Hence the motivation to increase the kV.
2.) Sputtering - the energy of the electron is sufficient to remove an atom from the surface of the material (most frequently the beam exit surface of the sample) . The cross-section for this has a threshold and then increases with Energy. Depending upon the cleanliness of your microscope you may or maynot see this effect. Hydrocarbon contamination does not sputter strongly, and thus acts like "glue" holding the surface atoms of your real sample in place, so to speak.
3.) Displacement - the energy of the electron is sufficient to knock an atom out of its location in a crystalline lattice inside of your sample. This is the highest energy process, and is the bane of materials scientists as they go to higher voltage instruments to look at thicker samples, or when high voltage is used for high resolution. This effect creates vacancies, interstitials etc.. .which are usually mobile. BTW, this is sometimes called Knock-on damage.
All of these effects are a function of atomic number, bond strength, lattice strength, dose, dose rate and kinetic energy of the electron beam. Each will manifest itself at different energies for different materials.
Because of all of these factors for each material there is an optimum voltage to operate at , which is at the local minimum created by the intersection of these 3 processes. The important point is that it is never zero in a typical TEM environment.
In your carbon nanotubes you are likely seeing #3. The simple experiment to do is, keeping the illumination conditions approximately constant, drop the kV in your instrument and observe the nano-tubes. You will find a voltage at which the damage as a function of time is minimized. My guess is that this will be ~ 90-100 kV. Because of the threshold, and the fact that it takes time for damage to accumulate, you can work at higher voltages for short periods of time before defects accumulate sufficiently to disrupt a given materials structure. Of course if your working with single walled carbon nanotubes this won't be very long.
I'll digup a few papers on sputtering/displacement damage that colleagues and I worked on a sometime ago on this and put it on the following WWW site later this morning if your interested in reading abit more.
http://tpm.amc.anl.gov/Lectures
There should be references to the sputtering/displacement damage calculations therein. For the radiolysis you will have to go to the life science EM literature, or hopefully, one of our life science colleagues will post a message.
Nestor Your Friendly Neighborhood SysOp
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==============================Original Headers============================== 17, 13 -- From zaluzec-at-microscopy.com Tue Jun 26 08:15:59 2007 17, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 17, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5QDFxo0030122 17, 13 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 08:15:59 -0500 17, 13 -- Mime-Version: 1.0 17, 13 -- Message-Id: {p06240800c2a6bae63abc-at-[206.69.208.22]} 17, 13 -- In-Reply-To: {200706261004.l5QA4f27007990-at-ns.microscopy.com} 17, 13 -- References: {200706261004.l5QA4f27007990-at-ns.microscopy.com} 17, 13 -- Date: Tue, 26 Jun 2007 08:15:58 -0500 17, 13 -- To: microscopy-at-microscopy.com 17, 13 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 17, 13 -- Subject: Re: [Microscopy] Explanation of CNT behaviour in TEMs 17, 13 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
I am looking for any advice/experience you might offer regarding the preparation of bones for SEM.
I have a researcher who is doing a project on how bones fracture following blunt force and other types of injury. She is also working to understand if she can tell if the fracture happened pre or postmortem. She is a forensic anthropologist who works for a local sheriff's office and while it's not quite CSI, it's as close as I am going to get.
Our first problem has come up with her test bones. She is using cow bones and some of them appear to have a lot of fat or other 'stuff' in them. Prior to coating for SEM, I put the bones in a VE for a couple of days to be sure they were dry and coatable. Even after several days in the VE, some of the samples still glisten and look 'wet' and juicy. Not ready to coat or put in my conventional SEM.
Looking for ideas about how to prepare these bones for SEM, alcohol soak?, detergent soak?, something else?
I am not a boneologist and would appreciate any ideas.
Thanks
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. Visit http://www.aidslifecycle.org for more information about the ride or http://www.aidslifecycle.org/5482 to make a donation.
==============================Original Headers============================== 10, 17 -- From jmkrupp-at-ucsc.edu Tue Jun 26 13:23:01 2007 10, 17 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5QIN0Dl030414 10, 17 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 13:23:01 -0500 10, 17 -- Received: from [128.114.125.2] (HELO ucsc.edu) 10, 17 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 10, 17 -- with ESMTPS id 19078601 for microscopy-at-microscopy.com; Tue, 26 Jun 2007 11:23:00 -0700 10, 17 -- Received: from [128.114.25.234] (account jmkrupp-at-ucsc.edu HELO [128.114.25.234]) 10, 17 -- by silver.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 10, 17 -- with ESMTPA id 135197892 for microscopy-at-microscopy.com; Tue, 26 Jun 2007 11:23:00 -0700 10, 17 -- Mime-Version: 1.0 10, 17 -- Message-Id: {p06230901c2a70812a1e3-at-[128.114.25.234]} 10, 17 -- Date: Tue, 26 Jun 2007 11:22:59 -0700 10, 17 -- To: microscopy-at-microscopy.com 10, 17 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 10, 17 -- Subject: SEM of bones 10, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I've done horse bone (cannon bone) looking for micro-fractures, and those were simply air-dried, no other treatment (except metal coating). This worked fine for a FESEM, but I suspect the pieces were much smaller than the ones you're dealing with. If you have fat on or in the bones, I'd try soaking them in chloroform for 30 minutes X 3 to a day or three, depending on the bone size, then air-dry. Chloroform works a treat to de-fat cheese (and "cheese"), and should work well for bone. It dries off nicely, once you get to the final change when there's only chloroform left. Using detergent would leave behind a detergent residue that would then need to be cleaned off, and EtOH or MeOH wouldn't get all the fat. t-BuOH might, though -- I haven't tried that.
Phil
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==============================Original Headers============================== 4, 22 -- From oshel1pe-at-cmich.edu Tue Jun 26 13:55:37 2007 4, 22 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5QItbab010388 4, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 13:55:37 -0500 4, 22 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 22 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id l5QJJuQI000673 4, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 15:19:58 -0400 4, 22 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 4, 22 -- Tue, 26 Jun 2007 14:55:33 -0400 4, 22 -- Mime-Version: 1.0 4, 22 -- Message-Id: {f06240810c2a70f7ec09e-at-[141.209.160.249]} 4, 22 -- In-Reply-To: {200706261828.l5QISxux004783-at-ns.microscopy.com} 4, 22 -- References: {200706261828.l5QISxux004783-at-ns.microscopy.com} 4, 22 -- Date: Tue, 26 Jun 2007 14:55:30 -0400 4, 22 -- To: Microscopy-at-microscopy.com 4, 22 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 22 -- Subject: Re: [Microscopy] SEM of bones 4, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 22 -- X-OriginalArrivalTime: 26 Jun 2007 18:55:33.0311 (UTC) FILETIME=[930234F0:01C7B823] 4, 22 -- X-CanItPRO-Stream: default 4, 22 -- X-Spam-Score: -3.9 () L_EXCH_MF,PORN_RP_JUICY 4, 22 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
I bet that this has been studied before and that the information is available in the open literature or pathology texts. A quick (several minute) search on Google gave me plenty of sites concerning osteology and osteologists.
I suspect that her study would best be done on human bones. A good contact for source of human bones may be the local morgue or medical school. Your colleague's work in forensics at a sheriff's office may lend credence to her request for human bones.
I wonder if an osteologist would think being called a boneologist is very humerus?
Regards,
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Happiness equals reality minus expectations." Tom Magliozzi
jmkrupp-at-ucsc.e du To gary.m.brown-at-exxonmobil.com 06/26/07 01:25 cc PM Subject [Microscopy] SEM of bones Please respond to jmkrupp-at-ucsc.e du
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Greetings:
I am looking for any advice/experience you might offer regarding the preparation of bones for SEM.
I have a researcher who is doing a project on how bones fracture following blunt force and other types of injury. She is also working to understand if she can tell if the fracture happened pre or postmortem. She is a forensic anthropologist who works for a local sheriff's office and while it's not quite CSI, it's as close as I am going to get.
Our first problem has come up with her test bones. She is using cow bones and some of them appear to have a lot of fat or other 'stuff' in them. Prior to coating for SEM, I put the bones in a VE for a couple of days to be sure they were dry and coatable. Even after several days in the VE, some of the samples still glisten and look 'wet' and juicy. Not ready to coat or put in my conventional SEM.
Looking for ideas about how to prepare these bones for SEM, alcohol soak?, detergent soak?, something else?
I am not a boneologist and would appreciate any ideas.
Thanks
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. Visit http://www.aidslifecycle.org for more information about the ride or http://www.aidslifecycle.org/5482 to make a donation.
==============================Original Headers============================== 10, 17 -- From jmkrupp-at-ucsc.edu Tue Jun 26 13:23:01 2007 10, 17 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5QIN0Dl030414 10, 17 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 13:23:01 -0500 10, 17 -- Received: from [128.114.125.2] (HELO ucsc.edu) 10, 17 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 10, 17 -- with ESMTPS id 19078601 for microscopy-at-microscopy.com; Tue, 26 Jun 2007 11:23:00 -0700 10, 17 -- Received: from [128.114.25.234] (account jmkrupp-at-ucsc.edu HELO [128.114.25.234]) 10, 17 -- by silver.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 10, 17 -- with ESMTPA id 135197892 for microscopy-at-microscopy.com; Tue, 26 Jun 2007 11:23:00 -0700 10, 17 -- Mime-Version: 1.0 10, 17 -- Message-Id: {p06230901c2a70812a1e3-at-[128.114.25.234]} 10, 17 -- Date: Tue, 26 Jun 2007 11:22:59 -0700 10, 17 -- To: microscopy-at-microscopy.com 10, 17 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 10, 17 -- Subject: SEM of bones 10, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 35, 19 -- From gary.m.brown-at-exxonmobil.com Tue Jun 26 14:09:05 2007 35, 19 -- Received: from dalspc01.exxonmobil.com (dalspc01.exxonmobil.com [131.126.223.1]) 35, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5QJ95GT022219 35, 19 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 14:09:05 -0500 35, 19 -- Received: from dalnmg04.na.xom.com (dalnmg04.na.xom.com [131.126.97.123]) 35, 19 -- by dalspc01.exxonmobil.com (Switch-3.1.11/Switch-3.1.11) with ESMTP id l5QJ92sL010433; 35, 19 -- Tue, 26 Jun 2007 14:09:04 -0500 (CDT) 35, 19 -- In-Reply-To: {200706261825.l5QIPmoY001274-at-ns.microscopy.com} 35, 19 -- Subject: Re: [Microscopy] SEM of bones 35, 19 -- Importance: 35, 19 -- To: jmkrupp-at-ucsc.edu, microscopy-at-microscopy.com 35, 19 -- X-Mailer: Lotus Notes 652HF109 January 14, 2005 35, 19 -- Message-ID: {OF1DA490F4.DB09F673-ON86257306.00672FCE-86257306.0069326A-at-exxonmobil.com} 35, 19 -- From: gary.m.brown-at-exxonmobil.com 35, 19 -- Date: Tue, 26 Jun 2007 14:09:02 -0500 35, 19 -- X-MIMETrack: Serialize by Router on Dalnmg04.na.xom.com/S/ExxonMobil(652FP1HF193|March 35, 19 -- 02, 2006) at 06/26/2007 02:09:04 PM 35, 19 -- MIME-Version: 1.0 35, 19 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Dear MSA, I have heard about using UV light to make carbon coated grids hydrophilic. Can anyone give me some specifics and perhaps some references? On or off list, as you wish.
Thank you very much, Jeannette
-- Jeannette Taylor, Technologist II IM&MF Cherry L. Emerson Hall, Room E106 Emory University 1515 Dickey Drive Atlanta, Georgia 30322
I once had a visitor from Kentucky Fried Chicken who wanted to look at fried coatings in the SEM. Of course I said sure thinking that we would look at a little bit and then get to eat the leftover chicken. No such luck! The coatings arrived without the chicken and with lots of grease.
I did as Phil suggested and put bits of the coating into chloroform to remove the frying grease and it worked great. Got some really interesting images of regular vs. extra crispy...
Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907
} From: {oshel1pe-at-cmich.edu} } Reply-To: {oshel1pe-at-cmich.edu} } Date: Tue, 26 Jun 2007 13:57:42 -0500 } To: {dsherman-at-purdue.edu} } Subject: [Microscopy] Re: SEM of bones } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Jon, } } I've done horse bone (cannon bone) looking for micro-fractures, and } those were simply air-dried, no other treatment (except metal } coating). This worked fine for a FESEM, but I suspect the pieces were } much smaller than the ones you're dealing with. } If you have fat on or in the bones, I'd try soaking them in } chloroform for 30 minutes X 3 to a day or three, depending on the } bone size, then air-dry. Chloroform works a treat to de-fat cheese } (and "cheese"), and should work well for bone. It dries off nicely, } once you get to the final change when there's only chloroform left. } Using detergent would leave behind a detergent residue that would } then need to be cleaned off, and EtOH or MeOH wouldn't get all the } fat. t-BuOH might, though -- I haven't tried that. } } Phil } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Greetings: } } } } I am looking for any advice/experience you might offer regarding the } } preparation of bones for SEM. } } } } I have a researcher who is doing a project on how bones fracture } } following blunt force and other types of injury. She is also working } } to understand if she can tell if the fracture happened pre or } } postmortem. She is a forensic anthropologist who works for a local } } sheriff's office and while it's not quite CSI, it's as close as I am } } going to get. } } } } Our first problem has come up with her test bones. She is using cow } } bones and some of them appear to have a lot of fat or other 'stuff' } } in them. Prior to coating for SEM, I put the bones in a VE for a } } couple of days to be sure they were dry and coatable. Even after } } several days in the VE, some of the samples still glisten and look } } 'wet' and juicy. Not ready to coat or put in my conventional SEM. } } } } Looking for ideas about how to prepare these bones for SEM, alcohol } } soak?, detergent soak?, something else? } } } } I am not a boneologist and would appreciate any ideas. } } } } Thanks } } } } Jon } } -- } } } } Jonathan Krupp } } Microscopy & Imaging Lab } } C230 Earth & Marine Science } } University of California } } Santa Cruz, CA 95064 } } (831) 459-2477 } } jmkrupp-at-ucsc.edu } } } } I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money } } for the San Francisco AIDS Foundation. Visit } } http://www.aidslifecycle.org for more information about the ride or } } http://www.aidslifecycle.org/5482 to make a donation. } -- } Philip Oshel } Microscopy Facility Supervisor } Biology Department } 024C Brooks Hall } Central Michigan University } Mt. Pleasant, MI 48859 } } ==============================Original Headers============================== } 4, 22 -- From oshel1pe-at-cmich.edu Tue Jun 26 13:55:37 2007 } 4, 22 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) } 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l5QItbab010388 } 4, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 13:55:37 -0500 } 4, 22 -- Received: from egatea.central.cmich.local ([141.209.15.74]) } 4, 22 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id l5QJJuQI000673 } 4, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 15:19:58 -0400 } 4, 22 -- Received: from [141.209.160.249] ([141.209.160.249]) by } egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); } 4, 22 -- Tue, 26 Jun 2007 14:55:33 -0400 } 4, 22 -- Mime-Version: 1.0 } 4, 22 -- Message-Id: {f06240810c2a70f7ec09e-at-[141.209.160.249]} } 4, 22 -- In-Reply-To: {200706261828.l5QISxux004783-at-ns.microscopy.com} } 4, 22 -- References: {200706261828.l5QISxux004783-at-ns.microscopy.com} } 4, 22 -- Date: Tue, 26 Jun 2007 14:55:30 -0400 } 4, 22 -- To: Microscopy-at-microscopy.com } 4, 22 -- From: Philip Oshel {oshel1pe-at-cmich.edu} } 4, 22 -- Subject: Re: [Microscopy] SEM of bones } 4, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } 4, 22 -- X-OriginalArrivalTime: 26 Jun 2007 18:55:33.0311 (UTC) } FILETIME=[930234F0:01C7B823] } 4, 22 -- X-CanItPRO-Stream: default } 4, 22 -- X-Spam-Score: -3.9 () L_EXCH_MF,PORN_RP_JUICY } 4, 22 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 22 -- From dsherman-at-purdue.edu Tue Jun 26 15:12:14 2007 9, 22 -- Received: from 1061exfe03a.itap.purdue.edu (1061exfe03a.itap.purdue.edu [128.210.1.10]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5QKCE0k014486 9, 22 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 15:12:14 -0500 9, 22 -- Received: from exchange.purdue.edu ([172.21.6.24]) by 1061exfe03a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 9, 22 -- Tue, 26 Jun 2007 16:12:15 -0400 9, 22 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 9, 22 -- Tue, 26 Jun 2007 20:12:14 +0000 9, 22 -- User-Agent: Microsoft-Entourage/11.3.3.061214 9, 22 -- Date: Tue, 26 Jun 2007 16:12:12 -0400 9, 22 -- Subject: Re: [Microscopy] Re: SEM of bones 9, 22 -- From: Debby Sherman {dsherman-at-purdue.edu} 9, 22 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 9, 22 -- Message-ID: {C2A6EB5C.1E7CC%dsherman-at-purdue.edu} 9, 22 -- Thread-Topic: [Microscopy] Re: SEM of bones 9, 22 -- Thread-Index: Ace4LkgKhswL+CQhEdyl/AARJN08Mg== 9, 22 -- In-Reply-To: {200706261857.l5QIvgTj014005-at-ns.microscopy.com} 9, 22 -- Mime-version: 1.0 9, 22 -- Content-type: text/plain; 9, 22 -- charset="US-ASCII" 9, 22 -- Content-transfer-encoding: 7bit 9, 22 -- X-OriginalArrivalTime: 26 Jun 2007 20:12:15.0068 (UTC) FILETIME=[49DE99C0:01C7B82E] ==============================End of - Headers==============================
Germicidal lamps (short wavelength UV light) can help make hydrophobic grids somewhat more hydrophilic. Basically, the UV ionizes the air, generating reactive molecules (like ozone) that then react with the grid surfaces (possibly cleaning them of undesirable organics such as oils). Before we started using the (much better) AC glow-discharge method to make grids hydrophilic, we did use this method. Place the coated grids on a filter paper (coated side facing the lamp) about 6-12 inches away from the lamp for 5 minutes. They should be used in a couple days as the effect wears off.
As with any new procedure, you will need to make adjustments to the distance and times since germicidal lamps vary in strength and they do become less effective as they age. I would start by placing the lamp 6 inches away from the grids and then withdrawing a grid every minute for so (4,5,6,7,8,9 min) and examining a stained preparation in the TEM looking for a relatively uniformly spread stain with a minimal number of patches of stain. Protect your eyes (goggles) and hands (gloves) from the UV and be aware that shiny (stainless steel) sturfaces reflect UV towards you.
JB
} Dear MSA, I have heard about using UV light to make carbon coated grids } hydrophilic. Can anyone give me some specifics and perhaps some references? } On or off list, as you wish. } } Thank you very much, Jeannette } } -- } Jeannette Taylor, Technologist II } IM&MF } Cherry L. Emerson Hall, Room E106 } Emory University } 1515 Dickey Drive } Atlanta, Georgia 30322
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
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Email: bhfrazer_us-at-yahoo.com Name: Brad Frazer
Title-Subject: [Filtered] WDS spectrometers
Question: Hello everyone,
I'm working on a soft x-ray project and I'm in need of a spectrometer.
I was thinking that a WDS spectrometer might be a good fit and was wondering if someone could help me decide if this is a good idea.
I'll be working at the C and N energies and ideally, would like an energy resolution of 1 eV or better. I can live with anything less than 10 eV.
Also, I would really like to find a parallel detection system that would give me a spectrum between 250 and 650 eV.
So I guess my questions are:
1.) What are typical and/or achievable energy resolutions in the 250 - 600 eV energy range for WDS spectrometers?
2.) Is parallel detection an option?
3.) Does anyone have a used WDS detector for sale that would work in this energy range? (Any SEM platform is fine)
I left an aluminium stub with a carbon tab on a shelf in the lab for 5 days, then I coated it shortly with carbon and analyzed the specimen by EDX+SEM at 20keV. I see all sorts of particles usually several µm big, but most interestingly the majority of the particles I see are composed of Si and Mg with a Si/Mg ratio of 1.5-1.6. No trace of anything else (SUTW window). I wonder what that can be. Can it be that the special glass used for the lab glassware contain Mg? Any idea?
Regards,
Stephane
____________________________________________________________________________________ Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. http://sims.yahoo.com/
==============================Original Headers============================== 7, 19 -- From nizets2-at-yahoo.com Wed Jun 27 03:40:04 2007 7, 19 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5R8e4cD001251 7, 19 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jun 2007 03:40:04 -0500 7, 19 -- Received: (qmail 30217 invoked by uid 60001); 27 Jun 2007 08:40:04 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 19 -- b=3WSZUaBP/EO0k1QhUCk1NrAx5TAT/z3v8Yyt8tAmc8YM2RyYKMPHu+wUGvB+CWQT5XNF3rxEppNPnHZxagPKieGXNqCPaALrGMdYHbhEoshg+Zq4wpZj8oHUmcjg3Fcno4K2Qd6OVhNdwmtBgFkFv/0nHNu1+0Lg0f/FSviud3Q=; 7, 19 -- X-YMail-OSG: HvDgizQVM1lA_yJfeNpzjBZsP6Kat2oRjv4qRjRLVXh2gcCUle7S8x8P31nXQiM6levpakEjZAZN72._UMM5zUvHSQYhmvFfJdgkhhtVxXzfnvS2G6dFzDzGWZtuNQ-- 7, 19 -- Received: from [80.122.101.102] by web37405.mail.mud.yahoo.com via HTTP; Wed, 27 Jun 2007 01:40:04 PDT 7, 19 -- Date: Wed, 27 Jun 2007 01:40:04 -0700 (PDT) 7, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 19 -- Subject: help: air contamination 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=iso-8859-1 7, 19 -- Content-Transfer-Encoding: 8bit 7, 19 -- Message-ID: {308194.29622.qm-at-web37405.mail.mud.yahoo.com} ==============================End of - Headers==============================
We have an investigator who would like to know if he can store glutaraldehyde-fixed mouse eye tissue while he collects samples from different litters born over a time span of 1 week or more. The gene that they are investigating affects membranes, and they are interested in doing TEM imaging of the photo-receptors and other membraneous components in the cells.
I know that standard TEM doctrine recommends processing the tissue immediately for optimal preservation of the ultrastructure, but does anyone out there have any experience with storing this kind of tissue, after glut fixation, for a week or more before proceeding to the osmium step? Also, many recommend storing the tissue in a buffer rinse rather than in the glut. Can anyone tell me if and why this is important?
As always, thanks so much for your suggestions,
Dotty Sorenson
Dorothy Sorenson Microscopy and Image-analysis Laboratory Department of Cell and Developmental Biology University Of Michigan Medical School A807 BSRB 109 Zina Pitcher Place Ann Arbor, MI 48109-2200 (734)763-1170 FAX (734)763-1166
==============================Original Headers============================== 9, 16 -- From dsoren-at-umich.edu Wed Jun 27 08:07:34 2007 9, 16 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81]) 9, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5RD7YFE019334 9, 16 -- for {microscopy-at-msa.microscopy.com} ; Wed, 27 Jun 2007 08:07:34 -0500 9, 16 -- Received: FROM [10.21.129.180] (host-18.subnet-17.med.umich.edu [141.214.17.18]) 9, 16 -- BY hackers.mr.itd.umich.edu ID 468260D9.9592C.10021 ; 9, 16 -- 27 Jun 2007 09:06:33 -0400 9, 16 -- Mime-Version: 1.0 (Apple Message framework v752.2) 9, 16 -- Content-Transfer-Encoding: 7bit 9, 16 -- Message-Id: {65170FE7-BB1A-4867-89B8-E3572F3317FB-at-umich.edu} 9, 16 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 9, 16 -- To: microscopy-at-msa.microscopy.com 9, 16 -- From: Dorothy Sorenson {dsoren-at-umich.edu} 9, 16 -- Subject: TEM-storage of glut-fixed retina 9, 16 -- Date: Wed, 27 Jun 2007 09:03:05 -0400 9, 16 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
Dear Dotty- Oh, you just brought back some unpleasant memories. A number of years ago, a post-doc here (who had been an EM tech before he went back to grad school, and so should have known better) brought me 80 rat eyes that he had accumulated over the course of a few months and stored in glut., in the 'frige. He had injected some sort of virus sub-retinally and was looking for the result. Well, after telling him (and his PI) that I had concerns about the quality of the fixation and possible artifacts, I processed the lot over the course of a couple of weeks (after } 2 months, I didn't think it would matter). The structure was fine, BUT everything was rife with "fixation pepper" and so nothing was publishable (see: Artifacts in Biological Electron Microscopy, Richard FE Crang & Karen L Klomparens, eds, Plenum Press, 1988). It looks a lot like fine Pb ppt, and I had to take pictures of unstained sections to prove to the post-doc and the PI that all of that gunk was NOT due to my staining technique. I think that storing the tissues for up to 1 week should be OK. I've had to do that on occasion and haven't seen any striking problems. If I have to store material before I can process it all the way, I prefer to do that in the post-osmium buffer. I don't know if that's just my personal voodoo, or if there is solid science backing it up. Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Weill-Cornell Medical College
I wish to construct an electron-optical column similar, in general terms, to a gun in a CRT. I recall, long ago, that there was a company that sold kits for doing this. A Constructor Set or Mecano for assembling prototype designs from components in the kit.
I can not locate this through Google. Does anyone know if the company still exists? Or, indeed, if you have such a kit you no longer need, would you be willing to part with it?
Thanks, Alwyn Eades -- ........... Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
==============================Original Headers============================== 5, 21 -- From jae5-at-lehigh.edu Wed Jun 27 08:27:19 2007 5, 21 -- Received: from rain.CC.Lehigh.EDU (rain.CC.Lehigh.EDU [128.180.39.20]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5RDRJDA001910 5, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Jun 2007 08:27:19 -0500 5, 21 -- Received: from [127.0.0.1] (Dyn055141.mat.Lehigh.EDU [128.180.55.141]) 5, 21 -- (authenticated bits=0) 5, 21 -- by rain.CC.Lehigh.EDU (8.14.1/8.14.1) with ESMTP id l5RDREr3001211 5, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 5, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Jun 2007 09:27:19 -0400 5, 21 -- Message-ID: {468265B2.3010400-at-lehigh.edu} 5, 21 -- Date: Wed, 27 Jun 2007 09:27:14 -0400 5, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu} 5, 21 -- Organization: Lehigh University 5, 21 -- User-Agent: Thunderbird 1.5.0.12 (Windows/20070509) 5, 21 -- MIME-Version: 1.0 5, 21 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com} 5, 21 -- Subject: Kit for electron optics 5, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 21 -- Content-Transfer-Encoding: 7bit 5, 21 -- X-Virus-Scanned: ClamAV 0.90.3/3540/Tue Jun 26 22:54:27 2007 on rain.CC.Lehigh.EDU 5, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Tom McKee has found my mystery substance. This is Talc, which clearly corresponds to the EDX analysis and which I shortly used last week in the lab.
Philip: I left the stub in the air on purpose. I wanted to verify if the particles in my samples could come from air contamination.
Thanks to all those who activated their neurons to help me.
Stephane
--- nizets2-at-yahoo.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear colleagues! } } I left an aluminium stub with a carbon tab on a } shelf } in the lab for 5 days, then I coated it shortly with } carbon and analyzed the specimen by EDX+SEM at } 20keV. } I see all sorts of particles usually several µm big, } but most interestingly the majority of the particles } I } see are composed of Si and Mg with a Si/Mg ratio of } 1.5-1.6. No trace of anything else (SUTW window). } I wonder what that can be. } Can it be that the special glass used for the lab } glassware contain Mg? } Any idea? } } Regards, } } Stephane } } } } } ____________________________________________________________________________________ } Moody friends. Drama queens. Your life? Nope! - } their life, your story. Play Sims Stories at Yahoo! } Games. } http://sims.yahoo.com/ } } ==============================Original } Headers============================== } 7, 19 -- From nizets2-at-yahoo.com Wed Jun 27 03:40:04 } 2007 } 7, 19 -- Received: from web37405.mail.mud.yahoo.com } (web37405.mail.mud.yahoo.com [209.191.91.137]) } 7, 19 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with SMTP id } l5R8e4cD001251 } 7, 19 -- for {microscopy-at-microscopy.com} ; Wed, 27 } Jun 2007 03:40:04 -0500 } 7, 19 -- Received: (qmail 30217 invoked by uid } 60001); 27 Jun 2007 08:40:04 -0000 } 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; } c=nofws; } 7, 19 -- s=s1024; d=yahoo.com; } 7, 19 -- } h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; } 7, 19 -- } b=3WSZUaBP/EO0k1QhUCk1NrAx5TAT/z3v8Yyt8tAmc8YM2RyYKMPHu+wUGvB+CWQT5XNF3rxEppNPnHZxagPKieGXNqCPaALrGMdYHbhEoshg+Zq4wpZj8oHUmcjg3Fcno4K2Qd6OVhNdwmtBgFkFv/0nHNu1+0Lg0f/FSviud3Q=; } 7, 19 -- X-YMail-OSG: } HvDgizQVM1lA_yJfeNpzjBZsP6Kat2oRjv4qRjRLVXh2gcCUle7S8x8P31nXQiM6levpakEjZAZN72._UMM5zUvHSQYhmvFfJdgkhhtVxXzfnvS2G6dFzDzGWZtuNQ-- } 7, 19 -- Received: from [80.122.101.102] by } web37405.mail.mud.yahoo.com via HTTP; Wed, 27 Jun } 2007 01:40:04 PDT } 7, 19 -- Date: Wed, 27 Jun 2007 01:40:04 -0700 (PDT) } 7, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 7, 19 -- Subject: help: air contamination } 7, 19 -- To: microscopy-at-microscopy.com } 7, 19 -- MIME-Version: 1.0 } 7, 19 -- Content-Type: text/plain; } charset=iso-8859-1 } 7, 19 -- Content-Transfer-Encoding: 8bit } 7, 19 -- Message-ID: } {308194.29622.qm-at-web37405.mail.mud.yahoo.com} } ==============================End of - } Headers============================== }
____________________________________________________________________________________ Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. http://answers.yahoo.com/dir/?link=list&sid=396545469
==============================Original Headers============================== 9, 20 -- From nizets2-at-yahoo.com Wed Jun 27 08:48:56 2007 9, 20 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5RDmtVO022340 9, 20 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jun 2007 08:48:56 -0500 9, 20 -- Received: (qmail 29535 invoked by uid 60001); 27 Jun 2007 13:48:54 -0000 9, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 20 -- s=s1024; d=yahoo.com; 9, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 9, 20 -- b=LLCgrCJlEOPgYzLM/qS8hOKS3j3l7lkPL27KPVETeuNv50hxLk4C6UlZuU+BC/ySKa8WJ7GY2QTBKAtTu5kwGzc0OhJrGT13uI4tErp7XfnCoNpfqs7R+ZYbWrOQj2L93tlzN3Pg70z40JHvbZ3XVEgcAKlWz3CkB5d/Tq26CGo=; 9, 20 -- X-YMail-OSG: VI79FuIVM1mkk.o6qjAytg7ONPsJVeCYMTLVfiPy048tmCkiM.uFgc3JovLdZhhpzg-- 9, 20 -- Received: from [80.122.101.102] by web37405.mail.mud.yahoo.com via HTTP; Wed, 27 Jun 2007 06:48:54 PDT 9, 20 -- Date: Wed, 27 Jun 2007 06:48:54 -0700 (PDT) 9, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 9, 20 -- Subject: Re: [Microscopy] help: air contamination...and the winner is... 9, 20 -- To: microscopy-at-microscopy.com 9, 20 -- In-Reply-To: {200706270846.l5R8kCnB008895-at-ns.microscopy.com} 9, 20 -- MIME-Version: 1.0 9, 20 -- Content-Type: text/plain; charset=iso-8859-1 9, 20 -- Content-Transfer-Encoding: 8bit 9, 20 -- Message-ID: {281332.28983.qm-at-web37405.mail.mud.yahoo.com} ==============================End of - Headers==============================
I would suggest that you go to the following site, http://www.jelight.com/uvo-ozone-cleaning.php. What you are doing is generating activated oxygen species and ozone. That is taking away the hydrocarbons and making the sample hydrophilic. Actually, the UVO process uses two wavelengths, one to break the molecule bonds and the other to create the activated oxygen species and ozone. It almost does the same thing as plasma cleaning and has been used for cleaning surfaces for a long time.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: jvtaylo-at-emory.edu [mailto:jvtaylo-at-emory.edu] Sent: Tuesday, June 26, 2007 12:46 PM To: Walck-at-SouthBayTech.com
Dear MSA, I have heard about using UV light to make carbon coated grids hydrophilic. Can anyone give me some specifics and perhaps some references? On or off list, as you wish.
Thank you very much, Jeannette
-- Jeannette Taylor, Technologist II IM&MF Cherry L. Emerson Hall, Room E106 Emory University 1515 Dickey Drive Atlanta, Georgia 30322
==============================Original Headers============================== 5, 24 -- From jvtaylo-at-emory.edu Tue Jun 26 14:41:20 2007 5, 24 -- Received: from pales.cc.emory.edu (pales.cc.emory.edu [170.140.52.55]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5QJfJJL002213 5, 24 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 14:41:20 -0500 5, 24 -- Received: from pales (pales [170.140.52.55]) 5, 24 -- by pales.cc.emory.edu (8.13.8/8.13.8) with ESMTP id l5QJfI2R029754 5, 24 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 15:41:19 -0400 (EDT) 5, 24 -- Received: from pales (unknown [127.0.0.1]) 5, 24 -- by pales (Symantec Mail Security) with ESMTP id D9080335F 5, 24 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 15:41:18 -0400 (EDT) 5, 24 -- X-AuditID: aa8c3437-000000040000058a-2a-46816bde7718 5, 24 -- Received: from [170.140.189.92] (emoryfloatdmz.cc.emory.edu [170.140.52.254]) 5, 24 -- by pales (Symantec Mail Security) with ESMTP id AF59D329F 5, 24 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jun 2007 15:41:18 -0400 (EDT) 5, 24 -- Message-ID: {46816BD9.3070704-at-emory.edu} 5, 24 -- Date: Tue, 26 Jun 2007 15:41:13 -0400 5, 24 -- From: Jeannette Taylor {jvtaylo-at-emory.edu} 5, 24 -- User-Agent: Thunderbird 1.5.0.12 (Windows/20070509) 5, 24 -- MIME-Version: 1.0 5, 24 -- To: Microscopy ListServe {microscopy-at-microscopy.com} 5, 24 -- Subject: hydrophilic carbon film 5, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- X-Brightmail-Tracker: AAAAAA== ==============================End of - Headers==============================
==============================Original Headers============================== 14, 22 -- From walck-at-southbaytech.com Wed Jun 27 12:11:26 2007 14, 22 -- Received: from flpvm09.prodigy.net (flpvm09.prodigy.net [207.115.20.39]) 14, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5RHBOEU006558 14, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Jun 2007 12:11:26 -0500 14, 22 -- X-ORBL: [64.169.217.123] 14, 22 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) 14, 22 -- by flpvm09.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id l5RHAsrk009152; 14, 22 -- Wed, 27 Jun 2007 10:10:55 -0700 14, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} 14, 22 -- To: {Microscopy-at-microscopy.com} 14, 22 -- Cc: {jvtaylo-at-emory.edu} 14, 22 -- Subject: RE: [Microscopy] hydrophilic carbon film 14, 22 -- Date: Wed, 27 Jun 2007 10:11:38 -0700 14, 22 -- Message-ID: {002301c7b8de$3c59d840$7801a8c0-at-dynamicbl8uno3} 14, 22 -- MIME-Version: 1.0 14, 22 -- Content-Type: text/plain; 14, 22 -- charset="US-ASCII" 14, 22 -- Content-Transfer-Encoding: 7bit 14, 22 -- X-Mailer: Microsoft Office Outlook 11 14, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 14, 22 -- In-Reply-To: {200706261945.l5QJjU5b007864-at-ns.microscopy.com} 14, 22 -- Thread-Index: Ace4Ko0sNisnF5mpQ9C5rnYIodsq9wADqo0w ==============================End of - Headers==============================
From cynthia_damian003-at-yahoo.co.in Wed Jun 27 12:33:15 2007 Return-Path: {cynthia_damian003-at-yahoo.co.in} Received: from web94303.mail.in2.yahoo.com (web94303.mail.in2.yahoo.com [203.104.16.213]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5RHXBBt018848 for {MicroscopyListserverArchive-at-microscopy.com} ; Wed, 27 Jun 2007 12:33:13 -0500 Received: (qmail 52826 invoked by uid 60001); 27 Jun 2007 17:33:10 -0000 DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=s1024; d=yahoo.co.in; h=X-YMail-OSG:Received:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; b=w8fz/tClREW48/YtIQxLNDANS13StHlBOPbmBhjmtYSOGvQiyfj/C8MhYxzwj3F3cPQ1bOaWMN2xDXjXuSemtcjJ4NKyX5soaZQ1QrnCX5nrFmADMdC1Up8dSbfucWK2rcW/iKlMbr6i9msT6ubDbbTVI3tNOUvc6nIowd/DoW8=; X-YMail-OSG: yZoVMM8VM1nZOaL_jCpcN1GyJZ5_vAFJPDNYs_ZGX0k.fBU3v4jZyg2O024x__VqLpehE_FkSiBD2UEJ4yNdIhxyTjebtCnD2HwS Received: from [196.201.94.24] by web94303.mail.in2.yahoo.com via HTTP; Wed, 27 Jun 2007 18:33:10 BST
Jeannette,
20+ years positive experience with UV-treated carbon films in a leading EM research group is summarized and capped by them in Burgess et al, Journal of Structural Biology 147 (2004) 247-258. I will email it to you offlist.
I gather it might be a bit tricky to find the exact UV light they used, or to find and verify a US equivalent. But the effort sounds well worth making.
You should also look on SPI's website for some experienced and thoughtful commentary on various methods for preparing hydrophilic, hydrophobic, plus- and minus-charged surfaces etc. I'm not veruy good at navigating the SPI site, but one of those sites is at http://www.2spi.com/catalog/grids/cusctgrd.html
-mike reedy-
At 2:45 PM -0500 6/26/07, jvtaylo-at-emory.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
They sell electron and ion guns but somthing like you are looking for too. I've never used them, but it seems to be interesting.
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
jae5-at-lehigh.edu a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } I wish to construct an electron-optical column similar, in general } terms, to a gun in a CRT. I recall, long ago, that there was a company } that sold kits for doing this. A Constructor Set or Mecano for } assembling prototype designs from components in the kit. } } I can not locate this through Google. Does anyone know if the company } still exists? Or, indeed, if you have such a kit you no longer need, } would you be willing to part with it? } } Thanks, } Alwyn Eades }
==============================Original Headers============================== 9, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Thu Jun 28 02:19:17 2007 9, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.152]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5S7JHtU018220 9, 29 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 02:19:17 -0500 9, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 9, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id l5S7J7l6032574 9, 29 -- ; Thu, 28 Jun 2007 09:19:08 +0200 (CEST) 9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id D4ED53EC085; 9, 29 -- Thu, 28 Jun 2007 09:18:21 +0200 (CEST) 9, 29 -- Message-ID: {468360D2.7030507-at-ipcms.u-strasbg.fr} 9, 29 -- Date: Thu, 28 Jun 2007 09:18:42 +0200 9, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 9, 29 -- User-Agent: Thunderbird 1.5.0.12 (X11/20070604) 9, 29 -- MIME-Version: 1.0 9, 29 -- To: jae5-at-lehigh.edu, Microscopy-at-microscopy.com 9, 29 -- Subject: Re: [Microscopy] Kit for electron optics 9, 29 -- References: {200706271331.l5RDVi6i017014-at-ns.microscopy.com} 9, 29 -- In-Reply-To: {200706271331.l5RDVi6i017014-at-ns.microscopy.com} 9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 29 -- Content-Transfer-Encoding: 8bit 9, 29 -- X-IPCMS-MailScanner: Found to be clean 9, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.152]); Thu, 28 Jun 2007 09:19:08 +0200 (CEST) 9, 29 -- X-Virus-Scanned: ClamAV 0.88.7/3545/Thu Jun 28 05:08:05 2007 on mr2.u-strasbg.fr 9, 29 -- X-Virus-Status: Clean 9, 29 -- X-Spam-Status: No, score=-0.2 required=5.0 tests=AWL autolearn=disabled 9, 29 -- version=3.1.8 9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr2.u-strasbg.fr ==============================End of - Headers==============================
Is it not the principle of plasma cleaners? Could it be that this works to clean samples too?
Regards,
Stephane
--- bozzola-at-siu.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Germicidal lamps (short wavelength UV light) can } help make } hydrophobic grids somewhat more hydrophilic. } Basically, the UV } ionizes the air, generating reactive molecules (like } ozone) that then } react with the grid surfaces (possibly cleaning them } of undesirable } organics such as oils). Before we started using the } (much better) AC } glow-discharge method to make grids hydrophilic, we } did use this } method. Place the coated grids on a filter paper } (coated side facing } the lamp) about 6-12 inches away from the lamp for 5 } minutes. They } should be used in a couple days as the effect wears } off. } } As with any new procedure, you will need to make } adjustments to the } distance and times since germicidal lamps vary in } strength and they } do become less effective as they age. I would start } by placing the } lamp 6 inches away from the grids and then } withdrawing a grid every } minute for so (4,5,6,7,8,9 min) and examining a } stained preparation } in the TEM looking for a relatively uniformly spread } stain with a } minimal number of patches of stain. Protect your } eyes (goggles) and } hands (gloves) from the UV and be aware that shiny } (stainless steel) } sturfaces reflect UV towards you. } } JB } } } Dear MSA, I have heard about using UV light to make } carbon coated grids } } hydrophilic. Can anyone give me some specifics and } perhaps some references? } } On or off list, as you wish. } } } } Thank you very much, Jeannette } } } } -- } } Jeannette Taylor, Technologist II } } IM&MF } } Cherry L. Emerson Hall, Room E106 } } Emory University } } 1515 Dickey Drive } } Atlanta, Georgia 30322 } } -- } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } John J. Bozzola, Ph.D., Director } Integrated Microscopy & Graphics Expertise (IMAGE) } Southern Illinois University } 750 Communications Drive - MC 4402 } Carbondale, IL 62901 } Telephone: 618-453-3730 } } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } ==============================Original } Headers============================== } 7, 19 -- From bozzola-at-siu.edu Tue Jun 26 15:26:53 } 2007 } 7, 19 -- Received: from abbmta2.siu.edu } (abbmta2.siu.edu [131.230.254.206]) } 7, 19 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l5QKQr9c026429 } 7, 19 -- for {Microscopy-at-msa.microscopy.com} ; Tue, } 26 Jun 2007 15:26:53 -0500 } 7, 19 -- Received: from [131.230.177.136] } (ws177136.microscope.siu.edu [131.230.177.136]) } 7, 19 -- by abbmta2.siu.edu } (Switch-3.2.5/Switch-3.2.5) with ESMTP id } l5QKQo8n027503 } 7, 19 -- for {Microscopy-at-msa.microscopy.com} ; Tue, } 26 Jun 2007 15:26:51 -0500 (CDT) } 7, 19 -- Mime-Version: 1.0 } 7, 19 -- Message-Id: } {a06240804c2a7221e6bca-at-[131.230.177.136]} } 7, 19 -- In-Reply-To: } {200706261942.l5QJgEiC003810-at-ns.microscopy.com} } 7, 19 -- References: } {200706261942.l5QJgEiC003810-at-ns.microscopy.com} } 7, 19 -- Date: Tue, 26 Jun 2007 15:26:50 -0500 } 7, 19 -- To: Microscopy-at-msa.microscopy.com } 7, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu} } 7, 19 -- Subject: Re: [Microscopy] hydrophilic } carbon film } 7, 19 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } 7, 19 -- X-Spam-Score: 0.00% } 7, 19 -- X-MASF: 0.00% } 7, 19 -- X-Whitelist: 0.00% } ==============================End of - } Headers============================== }
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==============================Original Headers============================== 8, 20 -- From nizets2-at-yahoo.com Thu Jun 28 02:51:01 2007 8, 20 -- Received: from web37413.mail.mud.yahoo.com (web37413.mail.mud.yahoo.com [209.191.91.145]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5S7p0Tt030228 8, 20 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 02:51:01 -0500 8, 20 -- Received: (qmail 10907 invoked by uid 60001); 28 Jun 2007 07:51:00 -0000 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 8, 20 -- s=s1024; d=yahoo.com; 8, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 8, 20 -- b=M0nR8BpJajOaQoOCKc7QKJXoS9ZnFuAlvVxiHxoaul7HXuEMdSD/1zHFhbpF3DNK2ed3q+X72+bJoYt3PdyEc2C9xcp2aqQMa22S5nZ/6gFo/onn6NsfUt+l6r9KRENCiLBk8b1owuHU999VgXpG3QtyeL4bw0XplH34Z7f3aWI=; 8, 20 -- X-YMail-OSG: Jm.JSDMVM1konyHVYx9PmlESRha7s.Ug_D8A5N71VtFgft5saMTRDEQbmdnPABdL4xYCxRoJ9gYhNOCweRrS_W2MHLaMmj7GG_DUeRdzmhW7hXkrFQKD3AiEf1Oa7qw3EHfkC89vVsEdoAtX25kqUPwL2XBi9WLWpYu5EeBiNoCqE4oq0wP0ryo- 8, 20 -- Received: from [80.122.101.102] by web37413.mail.mud.yahoo.com via HTTP; Thu, 28 Jun 2007 00:51:00 PDT 8, 20 -- Date: Thu, 28 Jun 2007 00:51:00 -0700 (PDT) 8, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 8, 20 -- Subject: Re: [Microscopy] Re: hydrophilic carbon film 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- In-Reply-To: {200706262031.l5QKVWhA001633-at-ns.microscopy.com} 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Type: text/plain; charset=iso-8859-1 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- Message-ID: {539183.10620.qm-at-web37413.mail.mud.yahoo.com} ==============================End of - Headers==============================
I discovered "demo" examples in our SEM and one example shows a TEM section with cells. The picture is very good (of course the demo example are rarely made of bad samples), very similar to what one can see by TEM. I wonder how it is possible to obtain good images of TEM section in SEM. I guess that it is only possible using BSE mode. Does anyone have experience with this? I wondered what would be the optimal thickness, resin, preparation. Are there special contrasting needs?
Best regards,
Stephane
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==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Thu Jun 28 02:58:01 2007 6, 19 -- Received: from web37409.mail.mud.yahoo.com (web37409.mail.mud.yahoo.com [209.191.91.141]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5S7w16N009404 6, 19 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 02:58:01 -0500 6, 19 -- Received: (qmail 32228 invoked by uid 60001); 28 Jun 2007 07:58:01 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 6, 19 -- b=u4XoE6v6nlTnajwbbAoehcKwLCvP7XjOCMr/mtf9pwHpGVKIZZcACsLIo6yShOCzrtp0ZheILYI5q5Gh42N0Tt6wNbUhacRcfi8fUpsvHJw+p6mH+foHOiCgOQAHfnduL44Wtt9XCeRjaqDWRFL5cp431pIAZq+OwXSBNdk4f8g=; 6, 19 -- X-YMail-OSG: plOWJ9YVM1kjUejWnu6By36NFm6GzAA.wisMEvhFbRs3fbR2u2Pb21LX9vfd_7f1b0BsE0dqQknpdHryRPKOUytidJNWe.bMN5Cr1HqYYlbfHoMcSRirq_B51dVECQ-- 6, 19 -- Received: from [80.122.101.102] by web37409.mail.mud.yahoo.com via HTTP; Thu, 28 Jun 2007 00:58:01 PDT 6, 19 -- Date: Thu, 28 Jun 2007 00:58:01 -0700 (PDT) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 -- Subject: thin sections in SEM 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- Message-ID: {281728.14030.qm-at-web37409.mail.mud.yahoo.com} ==============================End of - Headers==============================
You can use a plasma cleaner to do this, although normally you have to be careful you don't clean for longer than a few seconds if you're using grids with carbon films. However, with some plasma cleaner models there are holders available to buy that will decrease the rate, making the process more controllable.
Dr Mike Fay Nottingham Nanotechnology and Nanoscience Centre
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: 28 June 2007 08:55 To: michael.fay-at-nottingham.ac.uk
Is it not the principle of plasma cleaners? Could it be that this works to clean samples too?
Regards,
Stephane
--- bozzola-at-siu.edu wrote:
} } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Germicidal lamps (short wavelength UV light) can } help make } hydrophobic grids somewhat more hydrophilic. } Basically, the UV } ionizes the air, generating reactive molecules (like } ozone) that then } react with the grid surfaces (possibly cleaning them } of undesirable } organics such as oils). Before we started using the } (much better) AC } glow-discharge method to make grids hydrophilic, we } did use this } method. Place the coated grids on a filter paper } (coated side facing } the lamp) about 6-12 inches away from the lamp for 5 } minutes. They } should be used in a couple days as the effect wears } off. } } As with any new procedure, you will need to make } adjustments to the } distance and times since germicidal lamps vary in } strength and they } do become less effective as they age. I would start } by placing the } lamp 6 inches away from the grids and then } withdrawing a grid every } minute for so (4,5,6,7,8,9 min) and examining a } stained preparation } in the TEM looking for a relatively uniformly spread } stain with a } minimal number of patches of stain. Protect your } eyes (goggles) and } hands (gloves) from the UV and be aware that shiny } (stainless steel) } sturfaces reflect UV towards you. } } JB } } } Dear MSA, I have heard about using UV light to make } carbon coated grids } } hydrophilic. Can anyone give me some specifics and } perhaps some references? } } On or off list, as you wish. } } } } Thank you very much, Jeannette } } } } -- } } Jeannette Taylor, Technologist II } } IM&MF } } Cherry L. Emerson Hall, Room E106 } } Emory University } } 1515 Dickey Drive } } Atlanta, Georgia 30322 } } -- } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } John J. Bozzola, Ph.D., Director } Integrated Microscopy & Graphics Expertise (IMAGE) } Southern Illinois University } 750 Communications Drive - MC 4402 } Carbondale, IL 62901 } Telephone: 618-453-3730 } } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } ==============================Original } Headers============================== } 7, 19 -- From bozzola-at-siu.edu Tue Jun 26 15:26:53 } 2007 } 7, 19 -- Received: from abbmta2.siu.edu } (abbmta2.siu.edu [131.230.254.206]) } 7, 19 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l5QKQr9c026429 } 7, 19 -- for {Microscopy-at-msa.microscopy.com} ; Tue, } 26 Jun 2007 15:26:53 -0500 } 7, 19 -- Received: from [131.230.177.136] } (ws177136.microscope.siu.edu [131.230.177.136]) } 7, 19 -- by abbmta2.siu.edu } (Switch-3.2.5/Switch-3.2.5) with ESMTP id } l5QKQo8n027503 } 7, 19 -- for {Microscopy-at-msa.microscopy.com} ; Tue, } 26 Jun 2007 15:26:51 -0500 (CDT) } 7, 19 -- Mime-Version: 1.0 } 7, 19 -- Message-Id: } {a06240804c2a7221e6bca-at-[131.230.177.136]} } 7, 19 -- In-Reply-To: } {200706261942.l5QJgEiC003810-at-ns.microscopy.com} } 7, 19 -- References: } {200706261942.l5QJgEiC003810-at-ns.microscopy.com} } 7, 19 -- Date: Tue, 26 Jun 2007 15:26:50 -0500 } 7, 19 -- To: Microscopy-at-msa.microscopy.com } 7, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu} } 7, 19 -- Subject: Re: [Microscopy] hydrophilic } carbon film } 7, 19 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } 7, 19 -- X-Spam-Score: 0.00% } 7, 19 -- X-MASF: 0.00% } 7, 19 -- X-Whitelist: 0.00% } ==============================End of - } Headers============================== }
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==============================Original Headers============================== 8, 20 -- From nizets2-at-yahoo.com Thu Jun 28 02:51:01 2007 8, 20 -- Received: from web37413.mail.mud.yahoo.com (web37413.mail.mud.yahoo.com [209.191.91.145]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5S7p0Tt030228 8, 20 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 02:51:01 -0500 8, 20 -- Received: (qmail 10907 invoked by uid 60001); 28 Jun 2007 07:51:00 -0000 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 8, 20 -- s=s1024; d=yahoo.com; 8, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Con tent-Type:Content-Transfer-Encoding:Message-ID; 8, 20 -- b=M0nR8BpJajOaQoOCKc7QKJXoS9ZnFuAlvVxiHxoaul7HXuEMdSD/1zHFhbpF3DNK2ed3q+ X72+bJoYt3PdyEc2C9xcp2aqQMa22S5nZ/6gFo/onn6NsfUt+l6r9KRENCiLBk8b1owuHU99 9VgXpG3QtyeL4bw0XplH34Z7f3aWI=; 8, 20 -- X-YMail-OSG: Jm.JSDMVM1konyHVYx9PmlESRha7s.Ug_D8A5N71VtFgft5saMTRDEQbmdnPABdL4xYCxRoJ 9gYhNOCweRrS_W2MHLaMmj7GG_DUeRdzmhW7hXkrFQKD3AiEf1Oa7qw3EHfkC89vVsEdoAtX 25kqUPwL2XBi9WLWpYu5EeBiNoCqE4oq0wP0ryo- 8, 20 -- Received: from [80.122.101.102] by web37413.mail.mud.yahoo.com via HTTP; Thu, 28 Jun 2007 00:51:00 PDT 8, 20 -- Date: Thu, 28 Jun 2007 00:51:00 -0700 (PDT) 8, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 8, 20 -- Subject: Re: [Microscopy] Re: hydrophilic carbon film 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- In-Reply-To: {200706262031.l5QKVWhA001633-at-ns.microscopy.com} 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Type: text/plain; charset=iso-8859-1 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- Message-ID: {539183.10620.qm-at-web37413.mail.mud.yahoo.com} ==============================End of - Headers==============================
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==============================Original Headers============================== 19, 34 -- From Michael.Fay-at-nottingham.ac.uk Thu Jun 28 03:12:26 2007 19, 34 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk [128.243.44.5]) 19, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5S8CQ1Z021118 19, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 03:12:26 -0500 19, 34 -- Received: from suismtp2.ad.nottingham.ac.uk ([128.243.42.11]) 19, 34 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60) 19, 34 -- (envelope-from {Michael.Fay-at-nottingham.ac.uk} ) 19, 34 -- id 1I3p6x-0005aO-Ep 19, 34 -- for Microscopy-at-microscopy.com; Thu, 28 Jun 2007 09:12:19 +0100 19, 34 -- Received: from VUIEXCH1.ad.nottingham.ac.uk ([128.243.42.4]) by SUISMTP2.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.1830); 19, 34 -- Thu, 28 Jun 2007 09:12:19 +0100 19, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 34 -- Content-class: urn:content-classes:message 19, 34 -- MIME-Version: 1.0 19, 34 -- Content-Type: text/plain; 19, 34 -- charset="us-ascii" 19, 34 -- Subject: RE: [Microscopy] hydrophilic carbon film 19, 34 -- Date: Thu, 28 Jun 2007 09:12:18 +0100 19, 34 -- Message-ID: {0502910F1BFC8D47B48091E66D53E1660313C49E-at-VUIEXCH1.ad.nottingham.ac.uk} 19, 34 -- In-Reply-To: {200706280754.l5S7smSC003432-at-ns.microscopy.com} 19, 34 -- X-MS-Has-Attach: 19, 34 -- X-MS-TNEF-Correlator: 19, 34 -- Thread-Topic: [Microscopy] hydrophilic carbon film 19, 34 -- Thread-Index: Ace5WdTGoWNn8ZbfQemAjp62ME2ZuwAAXf1g 19, 34 -- References: {200706280754.l5S7smSC003432-at-ns.microscopy.com} 19, 34 -- From: "Fay Michael" {Michael.Fay-at-nottingham.ac.uk} 19, 34 -- To: {Microscopy-at-microscopy.com} 19, 34 -- X-OriginalArrivalTime: 28 Jun 2007 08:12:19.0254 (UTC) FILETIME=[0BFBF560:01C7B95C] 19, 34 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information 19, 34 -- X-UoN-MailScanner: Found to be clean 19, 34 -- X-UoN-MailScanner-From: michael.fay-at-nottingham.ac.uk 19, 34 -- X-Spam-Status: No 19, 34 -- Content-Transfer-Encoding: 8bit 19, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5S8CQ1Z021118 ==============================End of - Headers==============================
You can get very good SEM images from thin sections using a STEM detector. This is great for unstained material and gives very good contrast. Thus it is also good for ICC-labeled material where you have low contrast due to the absence of osmium during fixation.
Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907
} From: {nizets2-at-yahoo.com} } Reply-To: {nizets2-at-yahoo.com} } Date: Thu, 28 Jun 2007 02:59:46 -0500 } To: {dsherman-at-purdue.edu} } Subject: [Microscopy] thin sections in SEM } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all! } } I discovered "demo" examples in our SEM and one } example shows a TEM section with cells. The picture is } very good (of course the demo example are rarely made } of bad samples), very similar to what one can see by } TEM. I wonder how it is possible to obtain good images } of TEM section in SEM. } I guess that it is only possible using BSE mode. } Does anyone have experience with this? I wondered what } would be the optimal thickness, resin, preparation. } Are there special contrasting needs? } } Best regards, } } Stephane } } } } ______________________________________________________________________________ } ______ } Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, } news, photos & more. } http://mobile.yahoo.com/go?refer=1GNXIC } } ==============================Original Headers============================== } 6, 19 -- From nizets2-at-yahoo.com Thu Jun 28 02:58:01 2007 } 6, 19 -- Received: from web37409.mail.mud.yahoo.com } (web37409.mail.mud.yahoo.com [209.191.91.141]) } 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } l5S7w16N009404 } 6, 19 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 02:58:01 -0500 } 6, 19 -- Received: (qmail 32228 invoked by uid 60001); 28 Jun 2007 07:58:01 } -0000 } 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 6, 19 -- s=s1024; d=yahoo.com; } 6, 19 -- } h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content- } Transfer-Encoding:Message-ID; } 6, 19 -- } b=u4XoE6v6nlTnajwbbAoehcKwLCvP7XjOCMr/mtf9pwHpGVKIZZcACsLIo6yShOCzrtp0ZheILYI5 } q5Gh42N0Tt6wNbUhacRcfi8fUpsvHJw+p6mH+foHOiCgOQAHfnduL44Wtt9XCeRjaqDWRFL5cp431p } IAZq+OwXSBNdk4f8g=; } 6, 19 -- X-YMail-OSG: } plOWJ9YVM1kjUejWnu6By36NFm6GzAA.wisMEvhFbRs3fbR2u2Pb21LX9vfd_7f1b0BsE0dqQknpdH } ryRPKOUytidJNWe.bMN5Cr1HqYYlbfHoMcSRirq_B51dVECQ-- } 6, 19 -- Received: from [80.122.101.102] by web37409.mail.mud.yahoo.com via } HTTP; Thu, 28 Jun 2007 00:58:01 PDT } 6, 19 -- Date: Thu, 28 Jun 2007 00:58:01 -0700 (PDT) } 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 6, 19 -- Subject: thin sections in SEM } 6, 19 -- To: microscopy-at-microscopy.com } 6, 19 -- MIME-Version: 1.0 } 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 } 6, 19 -- Content-Transfer-Encoding: 8bit } 6, 19 -- Message-ID: {281728.14030.qm-at-web37409.mail.mud.yahoo.com} } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 22 -- From dsherman-at-purdue.edu Thu Jun 28 07:27:12 2007 8, 22 -- Received: from 1061exfe03.adpc.purdue.edu (1061exfe03.adpc.purdue.edu [128.210.63.225]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5SCRCnl011745 8, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 07:27:12 -0500 8, 22 -- Received: from exchange.purdue.edu ([172.21.6.24]) by 1061exfe03.adpc.purdue.edu with Microsoft SMTPSVC(6.0.3790.211); 8, 22 -- Thu, 28 Jun 2007 08:27:12 -0400 8, 22 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 8, 22 -- Thu, 28 Jun 2007 12:27:11 +0000 8, 22 -- User-Agent: Microsoft-Entourage/11.3.3.061214 8, 22 -- Date: Thu, 28 Jun 2007 08:27:11 -0400 8, 22 -- Subject: Re: [Microscopy] thin sections in SEM 8, 22 -- From: Debby Sherman {dsherman-at-purdue.edu} 8, 22 -- To: {nizets2-at-yahoo.com} , "message to: MSA list" {microscopy-at-microscopy.com} 8, 22 -- Message-ID: {C2A9215F.1E8DE%dsherman-at-purdue.edu} 8, 22 -- Thread-Topic: [Microscopy] thin sections in SEM 8, 22 -- Thread-Index: Ace5f6aT5OKJrCVyEdyl/AARJN08Mg== 8, 22 -- In-Reply-To: {200706280759.l5S7xkr0013000-at-ns.microscopy.com} 8, 22 -- Mime-version: 1.0 8, 22 -- Content-type: text/plain; 8, 22 -- charset="US-ASCII" 8, 22 -- Content-transfer-encoding: 7bit 8, 22 -- X-OriginalArrivalTime: 28 Jun 2007 12:27:12.0587 (UTC) FILETIME=[A78561B0:01C7B97F] ==============================End of - Headers==============================
Is anyone familiar with this simple digital microscope/camera? We wanted to evaluate it for possible use in our teaching (histology/ cell biology/etc.) labs, but the company is "unable" to lend it to us on a demo basis. The Website link is
http://www.tedpella.com/mscope_html/ProScope.htm
Thanks in advance for any info you can provide. MRA
Mark R. Adelman, Ph.D. Associate Professor of Anatomy, Physiology & Genetics Uniformed Services University of the Health Sciences 4301 Jones Bridge Road Bethesda, MD 20814-4799 USA Phone: 301-295-3208 FAX: 301-295-1786 Email: madelman-at-usuhs.mil Alternative work Email: adelman-at-educationalassistance.org Website: http://www.educationalassistance.org/ Home Email: mra-at-educationalassistance.org
==============================Original Headers============================== 5, 17 -- From madelman-at-usuhs.mil Thu Jun 28 07:40:50 2007 5, 17 -- Received: from mx.usuhs.mil (mx.usuhs.mil [131.158.7.228]) 5, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5SCeoWQ025854 5, 17 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 28 Jun 2007 07:40:50 -0500 5, 17 -- Received: from [131.158.180.144] ([131.158.180.144]) 5, 17 -- by mx.usuhs.mil (8.13.8/8.13.8) with ESMTP id l5S8udvh017521; 5, 17 -- Thu, 28 Jun 2007 08:56:39 GMT 5, 17 -- Mime-Version: 1.0 (Apple Message framework v752.2) 5, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 17 -- Message-Id: {E7D48885-5218-4A0C-AD64-D7E6AC8324D4-at-usuhs.mil} 5, 17 -- Cc: Mark Adelman {madelman-at-usuhs.mil} 5, 17 -- Content-Transfer-Encoding: 7bit 5, 17 -- From: Mark Adelman {madelman-at-usuhs.mil} 5, 17 -- Subject: Seeking comment on "ProScope HR" 5, 17 -- Date: Thu, 28 Jun 2007 08:41:08 -0400 5, 17 -- To: Microscopy ListServ {Microscopy-at-MSA.Microscopy.Com} 5, 17 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
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Email: zhouda-at-umsl.edu Name: Dan Zhou
Organization: University of Missouri - St. Louis
Title-Subject: [Filtered] Ultramicrotome
Question: Dear All,
Our Electron Image and Spectroscopy Technique Lab is looking for an used ultramicrotome to cut TEM thin sections or to prepare the surface for SEM observation.
Please let me know If anyone is selling or giving away an used ultramicrotome.
Thank you very much for any leads,
Dan
Laboratory Manager, Center for Nanoscience William L. Clay Building University of Missouri-St. Louis One University Boulevard St. Louis, Missouri 63121
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Email: emily.smith-at-nottingham.ac.uk Name: Emily Smith
Organization: University of Nottingham, School of Chemistry
Question: Hi I received an enquiry from a colleague for an electropolisher for TEM sample prep. Ideally he would like to buy secondhand as the new ones are out of the range of the budget. Has anyone got one available? or know of a source? Alternatively is there one in the North Oxfordshire area that could be utilised by my colleague for his prep? (I'm in Nottingham - he's down South!) Many thanks for any help Emily
I seem to have seen this scope before ... it might be one of the Scalar line. It seemed to work OK, but I would really like to see it in action. I would suggest that you ask Pella if you can have it on 5 day trial, against a provisional purchase order. That way, no one loses. If you like it, you just pay for it. If you don't you return it, but Pella has the security of knowing that they will get payment.
Hope this was helpful,
Best regards, Barbara Foster, President
We're moving July 1! Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 W: www.MicroscopyEducation.com
MME is now scheduling customized, on-site courses through September. Call us today for details.
P. S. Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Just call us here in the MME office for details.
At 07:50 AM 6/28/2007, madelman-at-usuhs.mil wrote:
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==============================Original Headers============================== 16, 18 -- From bfoster-at-mme1.com Thu Jun 28 08:35:52 2007 16, 18 -- Received: from smtp103.sbc.mail.mud.yahoo.com (smtp103.sbc.mail.mud.yahoo.com [68.142.198.202]) 16, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5SDZn7T006480 16, 18 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 08:35:51 -0500 16, 18 -- Message-Id: {200706281335.l5SDZn7T006480-at-ns.microscopy.com} 16, 18 -- Received: (qmail 24811 invoked from network); 28 Jun 2007 13:35:47 -0000 16, 18 -- Received: from unknown (HELO barbsd505.mme1.com) (bfostermme-at-sbcglobal.net-at-68.94.58.35 with login) 16, 18 -- by smtp103.sbc.mail.mud.yahoo.com with SMTP; 28 Jun 2007 13:35:47 -0000 16, 18 -- X-YMail-OSG: GCzWORIVM1k9kHhrKvf.eKY2VMW7Jo3u1qZgL5KdrkFUm9Q7C5BMfkss6fvChQkNux9ah0HqQSrZH8A1dH8mVo8E1L3LBC_LhMoIh1sOrvbrAph5UdMwA5uVqbFLZsrWbx7LcUgr5orGYqX75r5GEAqyC4XgWbeAhAWqEESfRdv6 16, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 16, 18 -- Date: Thu, 28 Jun 2007 08:34:38 -0500 16, 18 -- To: microscopy-at-microscopy.com 16, 18 -- From: Barbara Foster {bfoster-at-mme1.com} 16, 18 -- Subject: Re: [Microscopy] Seeking comment on "ProScope HR" 16, 18 -- In-Reply-To: {200706281242.l5SCgggp030144-at-ns.microscopy.com} 16, 18 -- References: {200706281242.l5SCgggp030144-at-ns.microscopy.com} 16, 18 -- Mime-Version: 1.0 16, 18 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
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Email: wangjinfengumsl-at-hotmail.com Name: Jinfeng Wang
Organization: University of Missouri
Title-Subject: [Filtered] photoluminescence spectra for solid state sample
Question: Dear All,
Does anyone have experience to do photoluminescence for solid state sample? Should I put the sample into solution first?
} -----Original Message----- } From: Michael.Fay-at-nottingham.ac.uk } [mailto:Michael.Fay-at-nottingham.ac.uk] } Sent: Thursday, June 28, 2007 3:13 AM } To: Dusevich, Vladimir } Subject: [Microscopy] RE: hydrophilic carbon film } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } You can use a plasma cleaner to do this, although normally } you have to be careful you don't clean for longer than a few } seconds if you're using grids with carbon films. However, } with some plasma cleaner models there are holders available } to buy that will decrease the rate, making the process more } controllable. } } Dr Mike Fay } Nottingham Nanotechnology and Nanoscience Centre } } } -----Original Message----- } X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] } Sent: 28 June 2007 08:55 } To: michael.fay-at-nottingham.ac.uk } Subject: [Microscopy] hydrophilic carbon film } } } } } -------------------------------------------------------------- } ---------- } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } ---------- } ---- } } Is it not the principle of plasma cleaners? } Could it be that this works to clean samples too? } } Regards, } } Stephane } } --- bozzola-at-siu.edu wrote: } } } } } } } } } } -------------------------------------------------------------- } ---------- } ---- } } The Microscopy ListServer -- CoSponsor: The } } Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } -------------------------------------------------------------- } ---------- } ---- } } } } Germicidal lamps (short wavelength UV light) can } } help make } } hydrophobic grids somewhat more hydrophilic. } } Basically, the UV } } ionizes the air, generating reactive molecules (like } } ozone) that then } } react with the grid surfaces (possibly cleaning them } } of undesirable } } organics such as oils). Before we started using the } } (much better) AC } } glow-discharge method to make grids hydrophilic, we } } did use this } } method. Place the coated grids on a filter paper } } (coated side facing } } the lamp) about 6-12 inches away from the lamp for 5 } } minutes. They } } should be used in a couple days as the effect wears } } off. } } } } As with any new procedure, you will need to make } } adjustments to the } } distance and times since germicidal lamps vary in } } strength and they } } do become less effective as they age. I would start } } by placing the } } lamp 6 inches away from the grids and then } } withdrawing a grid every } } minute for so (4,5,6,7,8,9 min) and examining a } } stained preparation } } in the TEM looking for a relatively uniformly spread } } stain with a } } minimal number of patches of stain. Protect your } } eyes (goggles) and } } hands (gloves) from the UV and be aware that shiny } } (stainless steel) } } sturfaces reflect UV towards you. } } } } JB } } } } } Dear MSA, I have heard about using UV light to make } } carbon coated grids } } } hydrophilic. Can anyone give me some specifics and } } perhaps some references? } } } On or off list, as you wish. } } } } } } Thank you very much, Jeannette } } } } } } -- } } } Jeannette Taylor, Technologist II } } } IM&MF } } } Cherry L. Emerson Hall, Room E106 } } } Emory University } } } 1515 Dickey Drive } } } Atlanta, Georgia 30322 } } } } -- } } } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } } } John J. Bozzola, Ph.D., Director } } Integrated Microscopy & Graphics Expertise (IMAGE) } } Southern Illinois University } } 750 Communications Drive - MC 4402 } } Carbondale, IL 62901 } } Telephone: 618-453-3730 } } } } } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } } } ==============================Original } } Headers============================== } } 7, 19 -- From bozzola-at-siu.edu Tue Jun 26 15:26:53 } } 2007 } } 7, 19 -- Received: from abbmta2.siu.edu } } (abbmta2.siu.edu [131.230.254.206]) } } 7, 19 -- by ns.microscopy.com } } (8.12.11.20060308/8.12.8) with ESMTP id } } l5QKQr9c026429 } } 7, 19 -- for {Microscopy-at-msa.microscopy.com} ; Tue, } } 26 Jun 2007 15:26:53 -0500 } } 7, 19 -- Received: from [131.230.177.136] } } (ws177136.microscope.siu.edu [131.230.177.136]) } } 7, 19 -- by abbmta2.siu.edu } } (Switch-3.2.5/Switch-3.2.5) with ESMTP id } } l5QKQo8n027503 } } 7, 19 -- for {Microscopy-at-msa.microscopy.com} ; Tue, } } 26 Jun 2007 15:26:51 -0500 (CDT) } } 7, 19 -- Mime-Version: 1.0 } } 7, 19 -- Message-Id: } } {a06240804c2a7221e6bca-at-[131.230.177.136]} } } 7, 19 -- In-Reply-To: } } {200706261942.l5QJgEiC003810-at-ns.microscopy.com} } } 7, 19 -- References: } } {200706261942.l5QJgEiC003810-at-ns.microscopy.com} } } 7, 19 -- Date: Tue, 26 Jun 2007 15:26:50 -0500 } } 7, 19 -- To: Microscopy-at-msa.microscopy.com } } 7, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu} } } 7, 19 -- Subject: Re: [Microscopy] hydrophilic } } carbon film } } 7, 19 -- Content-Type: text/plain; } } charset="us-ascii" ; format="flowed" } } 7, 19 -- X-Spam-Score: 0.00% } } 7, 19 -- X-MASF: 0.00% } } 7, 19 -- X-Whitelist: 0.00% } } ==============================End of - } } Headers============================== } } } } } } } ______________________________________________________________ } __________ } ____________ } Yahoo! oneSearch: Finally, mobile search } that gives answers, not web links. } http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC } } ==============================Original } Headers============================== } 8, 20 -- From nizets2-at-yahoo.com Thu Jun 28 02:51:01 2007 } 8, 20 -- Received: from web37413.mail.mud.yahoo.com } (web37413.mail.mud.yahoo.com [209.191.91.145]) } 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id l5S7p0Tt030228 } 8, 20 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 } 02:51:01 -0500 } 8, 20 -- Received: (qmail 10907 invoked by uid 60001); 28 Jun 2007 } 07:51:00 -0000 } 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 8, 20 -- s=s1024; d=yahoo.com; } 8, 20 -- } h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-V ersion:Con } tent-Type:Content-Transfer-Encoding:Message-ID; } 8, 20 -- } b=M0nR8BpJajOaQoOCKc7QKJXoS9ZnFuAlvVxiHxoaul7HXuEMdSD/1zHFhbpF } 3DNK2ed3q+ } X72+bJoYt3PdyEc2C9xcp2aqQMa22S5nZ/6gFo/onn6NsfUt+l6r9KRENCiLBk } 8b1owuHU99 } 9VgXpG3QtyeL4bw0XplH34Z7f3aWI=; } 8, 20 -- X-YMail-OSG: } Jm.JSDMVM1konyHVYx9PmlESRha7s.Ug_D8A5N71VtFgft5saMTRDEQbmdnPAB } dL4xYCxRoJ } 9gYhNOCweRrS_W2MHLaMmj7GG_DUeRdzmhW7hXkrFQKD3AiEf1Oa7qw3EHfkC8 } 9vVsEdoAtX } 25kqUPwL2XBi9WLWpYu5EeBiNoCqE4oq0wP0ryo- } 8, 20 -- Received: from [80.122.101.102] by } web37413.mail.mud.yahoo.com } via HTTP; Thu, 28 Jun 2007 00:51:00 PDT } 8, 20 -- Date: Thu, 28 Jun 2007 00:51:00 -0700 (PDT) } 8, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 8, 20 -- Subject: Re: [Microscopy] Re: hydrophilic carbon film } 8, 20 -- To: microscopy-at-microscopy.com } 8, 20 -- In-Reply-To: {200706262031.l5QKVWhA001633-at-ns.microscopy.com} } 8, 20 -- MIME-Version: 1.0 } 8, 20 -- Content-Type: text/plain; charset=iso-8859-1 } 8, 20 -- Content-Transfer-Encoding: 8bit } 8, 20 -- Message-ID: {539183.10620.qm-at-web37413.mail.mud.yahoo.com} } ==============================End of - } Headers============================== } } This message has been checked for viruses but the contents of } an attachment } may still contain software viruses, which could damage your } computer system: } you are advised to perform your own checks. Email } communications with the } University of Nottingham may be monitored as permitted by UK } legislation. } } } } ==============================Original } Headers============================== } 19, 34 -- From Michael.Fay-at-nottingham.ac.uk Thu Jun 28 03:12:26 2007 } 19, 34 -- Received: from smtp2.nottingham.ac.uk } (smtp2.nottingham.ac.uk [128.243.44.5]) } 19, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id l5S8CQ1Z021118 } 19, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Jun } 2007 03:12:26 -0500 } 19, 34 -- Received: from suismtp2.ad.nottingham.ac.uk } ([128.243.42.11]) } 19, 34 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60) } 19, 34 -- (envelope-from {Michael.Fay-at-nottingham.ac.uk} ) } 19, 34 -- id 1I3p6x-0005aO-Ep } 19, 34 -- for Microscopy-at-microscopy.com; Thu, 28 Jun 2007 } 09:12:19 +0100 } 19, 34 -- Received: from VUIEXCH1.ad.nottingham.ac.uk } ([128.243.42.4]) by SUISMTP2.ad.nottingham.ac.uk with } Microsoft SMTPSVC(6.0.3790.1830); } 19, 34 -- Thu, 28 Jun 2007 09:12:19 +0100 } 19, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 19, 34 -- Content-class: urn:content-classes:message } 19, 34 -- MIME-Version: 1.0 } 19, 34 -- Content-Type: text/plain; } 19, 34 -- charset="us-ascii" } 19, 34 -- Subject: RE: [Microscopy] hydrophilic carbon film } 19, 34 -- Date: Thu, 28 Jun 2007 09:12:18 +0100 } 19, 34 -- Message-ID: } {0502910F1BFC8D47B48091E66D53E1660313C49E-at-VUIEXCH1.ad.nottingh } am.ac.uk} } 19, 34 -- In-Reply-To: {200706280754.l5S7smSC003432-at-ns.microscopy.com} } 19, 34 -- X-MS-Has-Attach: } 19, 34 -- X-MS-TNEF-Correlator: } 19, 34 -- Thread-Topic: [Microscopy] hydrophilic carbon film } 19, 34 -- Thread-Index: Ace5WdTGoWNn8ZbfQemAjp62ME2ZuwAAXf1g } 19, 34 -- References: {200706280754.l5S7smSC003432-at-ns.microscopy.com} } 19, 34 -- From: "Fay Michael" {Michael.Fay-at-nottingham.ac.uk} } 19, 34 -- To: {Microscopy-at-microscopy.com} } 19, 34 -- X-OriginalArrivalTime: 28 Jun 2007 08:12:19.0254 } (UTC) FILETIME=[0BFBF560:01C7B95C] } 19, 34 -- X-UoN-MailScanner-Information: Please contact } staff-it-helpline-at-nottingham.ac.uk for more information } 19, 34 -- X-UoN-MailScanner: Found to be clean } 19, 34 -- X-UoN-MailScanner-From: michael.fay-at-nottingham.ac.uk } 19, 34 -- X-Spam-Status: No } 19, 34 -- Content-Transfer-Encoding: 8bit } 19, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit } by ns.microscopy.com id l5S8CQ1Z021118 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 7, 25 -- From DusevichV-at-umkc.edu Thu Jun 28 08:43:08 2007 7, 25 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5SDh8Zx016946 7, 25 -- for {microscopy-at-msa.microscopy.com} ; Thu, 28 Jun 2007 08:43:08 -0500 7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.1830); 7, 25 -- Thu, 28 Jun 2007 08:43:07 -0500 7, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 25 -- Content-class: urn:content-classes:message 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: text/plain; 7, 25 -- charset="us-ascii" 7, 25 -- Subject: RE: [Microscopy] RE: hydrophilic carbon film 7, 25 -- Date: Thu, 28 Jun 2007 08:43:07 -0500 7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB33ADCB7-at-KC-MSX1.kc.umkc.edu} 7, 25 -- In-Reply-To: {200706280812.l5S8Csag022136-at-ns.microscopy.com} 7, 25 -- X-MS-Has-Attach: 7, 25 -- X-MS-TNEF-Correlator: 7, 25 -- Thread-Topic: [Microscopy] RE: hydrophilic carbon film 7, 25 -- Thread-Index: Ace5XCL6ycmpw5RiR+qjdSiePMBGUAALcCKA 7, 25 -- References: {200706280812.l5S8Csag022136-at-ns.microscopy.com} 7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 7, 25 -- To: {Michael.Fay-at-nottingham.ac.uk} , {microscopy-at-msa.microscopy.com} 7, 25 -- X-OriginalArrivalTime: 28 Jun 2007 13:43:07.0570 (UTC) FILETIME=[4280A120:01C7B98A] 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5SDh8Zx016946 ==============================End of - Headers==============================
Without STEM detector it is very difficult (impossible?) to get decent image of thin sections in SEM. Anyway, I do use sometimes sections of calcified tissue in SEM. Hydroxyapatite is visible with BSE, and when grids are mounted on carbon, EDS spatial resolution in SEM is close to that of STEM. So, I can get advantage of superior resolution of STEM while working with multiple specimens in SEM.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] } Sent: Thursday, June 28, 2007 2:58 AM } To: Dusevich, Vladimir } Subject: [Microscopy] thin sections in SEM } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } Hi all! } } I discovered "demo" examples in our SEM and one example shows } a TEM section with cells. The picture is very good (of course } the demo example are rarely made of bad samples), very } similar to what one can see by TEM. I wonder how it is } possible to obtain good images of TEM section in SEM. } I guess that it is only possible using BSE mode. } Does anyone have experience with this? I wondered what would } be the optimal thickness, resin, preparation. } Are there special contrasting needs? } } Best regards, } } Stephane } } } } ______________________________________________________________ } ______________________ } Take the Internet to Go: Yahoo!Go puts the Internet in your } pocket: mail, news, photos & more. } http://mobile.yahoo.com/go?refer=1GNXIC } } ==============================Original } Headers============================== } 6, 19 -- From nizets2-at-yahoo.com Thu Jun 28 02:58:01 2007 6, } 19 -- Received: from web37409.mail.mud.yahoo.com } (web37409.mail.mud.yahoo.com [209.191.91.141]) } 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with SMTP id l5S7w16N009404 } 6, 19 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun } 2007 02:58:01 -0500 } 6, 19 -- Received: (qmail 32228 invoked by uid 60001); 28 Jun } 2007 07:58:01 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; } q=dns; c=nofws; } 6, 19 -- s=s1024; d=yahoo.com; } 6, 19 -- } h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Conte nt-Type:Content-Transfer-Encoding:Message-ID; } 6, 19 -- } b=u4XoE6v6nlTnajwbbAoehcKwLCvP7XjOCMr/mtf9pwHpGVKIZZcACsLIo6yS } hOCzrtp0ZheILYI5q5Gh42N0Tt6wNbUhacRcfi8fUpsvHJw+p6mH+foHOiCgOQ } AHfnduL44Wtt9XCeRjaqDWRFL5cp431pIAZq+OwXSBNdk4f8g=; } 6, 19 -- X-YMail-OSG: } plOWJ9YVM1kjUejWnu6By36NFm6GzAA.wisMEvhFbRs3fbR2u2Pb21LX9vfd_7 f1b0BsE0dqQknpdHryRPKOUytidJNWe.bMN5Cr1HqYYlbfHoMcSRirq_B51dVECQ} -- } 6, 19 -- Received: from [80.122.101.102] by } web37409.mail.mud.yahoo.com via HTTP; Thu, 28 Jun 2007 } 00:58:01 PDT 6, 19 -- Date: Thu, 28 Jun 2007 00:58:01 -0700 } (PDT) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 } -- Subject: thin sections in SEM 6, 19 -- To: } microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- } Content-Type: text/plain; charset=iso-8859-1 6, 19 -- } Content-Transfer-Encoding: 8bit 6, 19 -- Message-ID: } {281728.14030.qm-at-web37409.mail.mud.yahoo.com} } ==============================End of - } Headers============================== }
==============================Original Headers============================== 7, 25 -- From DusevichV-at-umkc.edu Thu Jun 28 08:54:07 2007 7, 25 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5SDs7fH010545 7, 25 -- for {microscopy-at-msa.microscopy.com} ; Thu, 28 Jun 2007 08:54:07 -0500 7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.1830); 7, 25 -- Thu, 28 Jun 2007 08:54:06 -0500 7, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 25 -- Content-class: urn:content-classes:message 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: text/plain; 7, 25 -- charset="us-ascii" 7, 25 -- Subject: RE: [Microscopy] thin sections in SEM 7, 25 -- Date: Thu, 28 Jun 2007 08:54:06 -0500 7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB33ADCB8-at-KC-MSX1.kc.umkc.edu} 7, 25 -- In-Reply-To: {200706280758.l5S7wOhw010415-at-ns.microscopy.com} 7, 25 -- X-MS-Has-Attach: 7, 25 -- X-MS-TNEF-Correlator: 7, 25 -- Thread-Topic: [Microscopy] thin sections in SEM 7, 25 -- Thread-Index: Ace5WhsAXenCsudHSf28cJUXrL+R5QAMbCJg 7, 25 -- References: {200706280758.l5S7wOhw010415-at-ns.microscopy.com} 7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 7, 25 -- To: {nizets2-at-yahoo.com} , {microscopy-at-msa.microscopy.com} 7, 25 -- X-OriginalArrivalTime: 28 Jun 2007 13:54:06.0765 (UTC) FILETIME=[CB69CDD0:01C7B98B] 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5SDs7fH010545 ==============================End of - Headers==============================
It might be good if the power (real, or setting) and Make/type of plasma cleaner are specified for these discussions.
For the record, I use a Harrick PDC-3XG, and use it on the LOW setting (nominally 6.8W - see mfg link below) for ~15 sec and get nicely wettable pure carbon films. I apply the power as soon as the pump sounds "right" (about 30 sec, probably ~ 0.1 Torr, glow discharge only happens in a small window of pressure in these conditions) and if there is a magenta glow I shut off the pump and time 15 sec. This discharge occurs in the residual AIR. My films are always in the 5-10nm range (by Film Thickness monitor and/or color). I treat grids multiple times with no apparent degradation. I don't know how long the effect lasts - I see various things suggesting that it lasts minutes to weeks - but I retreat if they have been sitting more than 30 min because it is really easy and they always work.
(The current model for this unit seems to be PDC-32G)
I have no connection to this company. The manufacturer website is: http://www.harrickplasma.com/products_cleaners.php
This unit has a needle valve fitting on the door so that materials can be admitted to alter the surface properties.
Hope this helps.
Dale Callaham The University of Massachusetts -at- Amherst
Michael.Fay-at-nottingham.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } You can use a plasma cleaner to do this, although normally you have to } be careful you don't clean for longer than a few seconds if you're using } grids with carbon films. However, with some plasma cleaner models there } are holders available to buy that will decrease the rate, making the } process more controllable. } } Dr Mike Fay } Nottingham Nanotechnology and Nanoscience Centre } } } -----Original Message----- } X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] } Sent: 28 June 2007 08:55 } To: michael.fay-at-nottingham.ac.uk } Subject: [Microscopy] hydrophilic carbon film } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } Is it not the principle of plasma cleaners? } Could it be that this works to clean samples too? } } Regards, } } Stephane } } --- bozzola-at-siu.edu wrote: } } } } } } } } ------------------------------------------------------------------------ } ---- } } The Microscopy ListServer -- CoSponsor: The } } Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } Germicidal lamps (short wavelength UV light) can } } help make } } hydrophobic grids somewhat more hydrophilic. } } Basically, the UV } } ionizes the air, generating reactive molecules (like } } ozone) that then } } react with the grid surfaces (possibly cleaning them } } of undesirable } } organics such as oils). Before we started using the } } (much better) AC } } glow-discharge method to make grids hydrophilic, we } } did use this } } method. Place the coated grids on a filter paper } } (coated side facing } } the lamp) about 6-12 inches away from the lamp for 5 } } minutes. They } } should be used in a couple days as the effect wears } } off. } } } } As with any new procedure, you will need to make } } adjustments to the } } distance and times since germicidal lamps vary in } } strength and they } } do become less effective as they age. I would start } } by placing the } } lamp 6 inches away from the grids and then } } withdrawing a grid every } } minute for so (4,5,6,7,8,9 min) and examining a } } stained preparation } } in the TEM looking for a relatively uniformly spread } } stain with a } } minimal number of patches of stain. Protect your } } eyes (goggles) and } } hands (gloves) from the UV and be aware that shiny } } (stainless steel) } } sturfaces reflect UV towards you. } } } } JB } } } } } Dear MSA, I have heard about using UV light to make } } carbon coated grids } } } hydrophilic. Can anyone give me some specifics and } } perhaps some references? } } } On or off list, as you wish. } } } } } } Thank you very much, Jeannette } } } } } } -- } } } Jeannette Taylor, Technologist II } } } IM&MF } } } Cherry L. Emerson Hall, Room E106 } } } Emory University } } } 1515 Dickey Drive } } } Atlanta, Georgia 30322 } } -- } } } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } John J. Bozzola, Ph.D., Director } } Integrated Microscopy & Graphics Expertise (IMAGE) } } Southern Illinois University } } 750 Communications Drive - MC 4402 } } Carbondale, IL 62901 } } Telephone: 618-453-3730 } } } } } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } } } ==============================Original } } Headers============================== } } 7, 19 -- From bozzola-at-siu.edu Tue Jun 26 15:26:53 } } 2007 } } 7, 19 -- Received: from abbmta2.siu.edu } } (abbmta2.siu.edu [131.230.254.206]) } } 7, 19 -- by ns.microscopy.com } } (8.12.11.20060308/8.12.8) with ESMTP id } } l5QKQr9c026429 } } 7, 19 -- for {Microscopy-at-msa.microscopy.com} ; Tue, } } 26 Jun 2007 15:26:53 -0500 } } 7, 19 -- Received: from [131.230.177.136] } } (ws177136.microscope.siu.edu [131.230.177.136]) } } 7, 19 -- by abbmta2.siu.edu } } (Switch-3.2.5/Switch-3.2.5) with ESMTP id } } l5QKQo8n027503 } } 7, 19 -- for {Microscopy-at-msa.microscopy.com} ; Tue, } } 26 Jun 2007 15:26:51 -0500 (CDT) } } 7, 19 -- Mime-Version: 1.0 } } 7, 19 -- Message-Id: } } {a06240804c2a7221e6bca-at-[131.230.177.136]} } } 7, 19 -- In-Reply-To: } } {200706261942.l5QJgEiC003810-at-ns.microscopy.com} } } 7, 19 -- References: } } {200706261942.l5QJgEiC003810-at-ns.microscopy.com} } } 7, 19 -- Date: Tue, 26 Jun 2007 15:26:50 -0500 } } 7, 19 -- To: Microscopy-at-msa.microscopy.com } } 7, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu} } } 7, 19 -- Subject: Re: [Microscopy] hydrophilic } } carbon film } } 7, 19 -- Content-Type: text/plain; } } charset="us-ascii" ; format="flowed" } } 7, 19 -- X-Spam-Score: 0.00% } } 7, 19 -- X-MASF: 0.00% } } 7, 19 -- X-Whitelist: 0.00% } } ==============================End of - } } Headers============================== } } } } } } } ________________________________________________________________________ } ____________ } Yahoo! oneSearch: Finally, mobile search } that gives answers, not web links. } http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC } } ==============================Original } Headers============================== } 8, 20 -- From nizets2-at-yahoo.com Thu Jun 28 02:51:01 2007 } 8, 20 -- Received: from web37413.mail.mud.yahoo.com } (web37413.mail.mud.yahoo.com [209.191.91.145]) } 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id l5S7p0Tt030228 } 8, 20 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 } 02:51:01 -0500 } 8, 20 -- Received: (qmail 10907 invoked by uid 60001); 28 Jun 2007 } 07:51:00 -0000 } 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 8, 20 -- s=s1024; d=yahoo.com; } 8, 20 -- } h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Con } tent-Type:Content-Transfer-Encoding:Message-ID; } 8, 20 -- } b=M0nR8BpJajOaQoOCKc7QKJXoS9ZnFuAlvVxiHxoaul7HXuEMdSD/1zHFhbpF3DNK2ed3q+ } X72+bJoYt3PdyEc2C9xcp2aqQMa22S5nZ/6gFo/onn6NsfUt+l6r9KRENCiLBk8b1owuHU99 } 9VgXpG3QtyeL4bw0XplH34Z7f3aWI=; } 8, 20 -- X-YMail-OSG: } Jm.JSDMVM1konyHVYx9PmlESRha7s.Ug_D8A5N71VtFgft5saMTRDEQbmdnPABdL4xYCxRoJ } 9gYhNOCweRrS_W2MHLaMmj7GG_DUeRdzmhW7hXkrFQKD3AiEf1Oa7qw3EHfkC89vVsEdoAtX } 25kqUPwL2XBi9WLWpYu5EeBiNoCqE4oq0wP0ryo- } 8, 20 -- Received: from [80.122.101.102] by web37413.mail.mud.yahoo.com } via HTTP; Thu, 28 Jun 2007 00:51:00 PDT } 8, 20 -- Date: Thu, 28 Jun 2007 00:51:00 -0700 (PDT) } 8, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 8, 20 -- Subject: Re: [Microscopy] Re: hydrophilic carbon film } 8, 20 -- To: microscopy-at-microscopy.com } 8, 20 -- In-Reply-To: {200706262031.l5QKVWhA001633-at-ns.microscopy.com} } 8, 20 -- MIME-Version: 1.0 } 8, 20 -- Content-Type: text/plain; charset=iso-8859-1 } 8, 20 -- Content-Transfer-Encoding: 8bit } 8, 20 -- Message-ID: {539183.10620.qm-at-web37413.mail.mud.yahoo.com} } ==============================End of - } Headers============================== } } This message has been checked for viruses but the contents of an attachment } may still contain software viruses, which could damage your computer system: } you are advised to perform your own checks. Email communications with the } University of Nottingham may be monitored as permitted by UK legislation. } } } } ==============================Original Headers============================== } 19, 34 -- From Michael.Fay-at-nottingham.ac.uk Thu Jun 28 03:12:26 2007 } 19, 34 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk [128.243.44.5]) } 19, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5S8CQ1Z021118 } 19, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 03:12:26 -0500 } 19, 34 -- Received: from suismtp2.ad.nottingham.ac.uk ([128.243.42.11]) } 19, 34 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60) } 19, 34 -- (envelope-from {Michael.Fay-at-nottingham.ac.uk} ) } 19, 34 -- id 1I3p6x-0005aO-Ep } 19, 34 -- for Microscopy-at-microscopy.com; Thu, 28 Jun 2007 09:12:19 +0100 } 19, 34 -- Received: from VUIEXCH1.ad.nottingham.ac.uk ([128.243.42.4]) by SUISMTP2.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.1830); } 19, 34 -- Thu, 28 Jun 2007 09:12:19 +0100 } 19, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 19, 34 -- Content-class: urn:content-classes:message } 19, 34 -- MIME-Version: 1.0 } 19, 34 -- Content-Type: text/plain; } 19, 34 -- charset="us-ascii" } 19, 34 -- Subject: RE: [Microscopy] hydrophilic carbon film } 19, 34 -- Date: Thu, 28 Jun 2007 09:12:18 +0100 } 19, 34 -- Message-ID: {0502910F1BFC8D47B48091E66D53E1660313C49E-at-VUIEXCH1.ad.nottingham.ac.uk} } 19, 34 -- In-Reply-To: {200706280754.l5S7smSC003432-at-ns.microscopy.com} } 19, 34 -- X-MS-Has-Attach: } 19, 34 -- X-MS-TNEF-Correlator: } 19, 34 -- Thread-Topic: [Microscopy] hydrophilic carbon film } 19, 34 -- Thread-Index: Ace5WdTGoWNn8ZbfQemAjp62ME2ZuwAAXf1g } 19, 34 -- References: {200706280754.l5S7smSC003432-at-ns.microscopy.com} } 19, 34 -- From: "Fay Michael" {Michael.Fay-at-nottingham.ac.uk} } 19, 34 -- To: {Microscopy-at-microscopy.com} } 19, 34 -- X-OriginalArrivalTime: 28 Jun 2007 08:12:19.0254 (UTC) FILETIME=[0BFBF560:01C7B95C] } 19, 34 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information } 19, 34 -- X-UoN-MailScanner: Found to be clean } 19, 34 -- X-UoN-MailScanner-From: michael.fay-at-nottingham.ac.uk } 19, 34 -- X-Spam-Status: No } 19, 34 -- Content-Transfer-Encoding: 8bit } 19, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5S8CQ1Z021118 } ==============================End of - Headers==============================
==============================Original Headers============================== 12, 23 -- From dac-at-research.umass.edu Thu Jun 28 09:12:58 2007 12, 23 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5SECwc5023452 12, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 09:12:58 -0500 12, 23 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 12, 23 -- (authenticated bits=0) 12, 23 -- by race5.oit.umass.edu (8.13.7/8.13.7) with ESMTP id l5SECu32010629 12, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 12, 23 -- Thu, 28 Jun 2007 10:12:57 -0400 12, 23 -- Message-ID: {4683D0A2.8070408-at-research.umass.edu} 12, 23 -- Date: Thu, 28 Jun 2007 10:15:46 -0500 12, 23 -- From: Dale Callaham {dac-at-research.umass.edu} 12, 23 -- Reply-To: dac-at-research.umass.edu 12, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.4) Gecko/20070509 SeaMonkey/1.1.2 12, 23 -- MIME-Version: 1.0 12, 23 -- To: Michael.Fay-at-nottingham.ac.uk, 12, 23 -- Microscopy Listserver {Microscopy-at-microscopy.com} 12, 23 -- Subject: Re: [Microscopy] RE: hydrophilic carbon film 12, 23 -- References: {200706280816.l5S8GcTU030607-at-ns.microscopy.com} 12, 23 -- In-Reply-To: {200706280816.l5S8GcTU030607-at-ns.microscopy.com} 12, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 23 -- Content-Transfer-Encoding: 7bit 12, 23 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Have you tried lowering the KV? We recently got a FE SEM with STEM capabilities and have experimented around with nanomaterials deposited on C-caoted Cu grids under all KeV's and with both STEM and SE detection. You never know what you will find but I've been amazed at some of the SE images.
Also, to boost the SE signal and if you have other samples, you could coat the section with a metal to improve the SE signal, especially at lower KeV's.
Thoughts from the dark side... Lou Ross
On 6/28/07 2:59 AM, "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all! } } I discovered "demo" examples in our SEM and one } example shows a TEM section with cells. The picture is } very good (of course the demo example are rarely made } of bad samples), very similar to what one can see by } TEM. I wonder how it is possible to obtain good images } of TEM section in SEM. } I guess that it is only possible using BSE mode. } Does anyone have experience with this? I wondered what } would be the optimal thickness, resin, preparation. } Are there special contrasting needs? } } Best regards, } } Stephane } } } } ______________________________________________________________________________ } ______ } Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, } news, photos & more. } http://mobile.yahoo.com/go?refer=1GNXIC } } ==============================Original Headers============================== } 6, 19 -- From nizets2-at-yahoo.com Thu Jun 28 02:58:01 2007 } 6, 19 -- Received: from web37409.mail.mud.yahoo.com } (web37409.mail.mud.yahoo.com [209.191.91.141]) } 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } l5S7w16N009404 } 6, 19 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 02:58:01 -0500 } 6, 19 -- Received: (qmail 32228 invoked by uid 60001); 28 Jun 2007 07:58:01 } -0000 } 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 6, 19 -- s=s1024; d=yahoo.com; } 6, 19 -- } h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content- } Transfer-Encoding:Message-ID; } 6, 19 -- } b=u4XoE6v6nlTnajwbbAoehcKwLCvP7XjOCMr/mtf9pwHpGVKIZZcACsLIo6yShOCzrtp0ZheILYI5 } q5Gh42N0Tt6wNbUhacRcfi8fUpsvHJw+p6mH+foHOiCgOQAHfnduL44Wtt9XCeRjaqDWRFL5cp431p } IAZq+OwXSBNdk4f8g=; } 6, 19 -- X-YMail-OSG: } plOWJ9YVM1kjUejWnu6By36NFm6GzAA.wisMEvhFbRs3fbR2u2Pb21LX9vfd_7f1b0BsE0dqQknpdH } ryRPKOUytidJNWe.bMN5Cr1HqYYlbfHoMcSRirq_B51dVECQ-- } 6, 19 -- Received: from [80.122.101.102] by web37409.mail.mud.yahoo.com via } HTTP; Thu, 28 Jun 2007 00:58:01 PDT } 6, 19 -- Date: Thu, 28 Jun 2007 00:58:01 -0700 (PDT) } 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 6, 19 -- Subject: thin sections in SEM } 6, 19 -- To: microscopy-at-microscopy.com } 6, 19 -- MIME-Version: 1.0 } 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 } 6, 19 -- Content-Transfer-Encoding: 8bit } 6, 19 -- Message-ID: {281728.14030.qm-at-web37409.mail.mud.yahoo.com} } ==============================End of - Headers============================== }
Senior Electron Microscope Specialist Electron Microscopy Core Facility W136 Veterinary Medicine University of Missouri Columbia, MO 65211-5120 (573) 882-4777, fax 884=2227 email: rosslm-at-missouri.edu http://www.emc.missouri.edu
==============================Original Headers============================== 7, 22 -- From RossLM-at-missouri.edu Thu Jun 28 09:18:25 2007 7, 22 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5SEIP6v030932 7, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 09:18:25 -0500 7, 22 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 22 -- Thu, 28 Jun 2007 09:18:25 -0500 7, 22 -- Received: from 128.206.79.82 ([128.206.79.82]) by UM-XMAIL06.um.umsystem.edu ([209.106.228.42]) via Exchange Front-End Server webmail.um.umsystem.edu ([209.106.228.21]) with Microsoft Exchange Server HTTP-DAV ; 7, 22 -- Thu, 28 Jun 2007 14:18:25 +0000 7, 22 -- User-Agent: Microsoft-Entourage/11.3.3.061214 7, 22 -- Date: Thu, 28 Jun 2007 09:18:23 -0500 7, 22 -- Subject: Re: [Microscopy] thin sections in SEM 7, 22 -- From: Lou Ross {rosslm-at-missouri.edu} 7, 22 -- To: {Microscopy-at-microscopy.com} 7, 22 -- Message-ID: {C2A92D5F.A564%rosslm-at-missouri.edu} 7, 22 -- Thread-Topic: [Microscopy] thin sections in SEM 7, 22 -- Thread-Index: Ace5WjVNAPIX4apFSp+zEQntASRUYAANPobH 7, 22 -- In-Reply-To: {200706280759.l5S7x8iu011319-at-ns.microscopy.com} 7, 22 -- Mime-version: 1.0 7, 22 -- Content-type: text/plain; 7, 22 -- charset="US-ASCII" 7, 22 -- Content-transfer-encoding: 7bit 7, 22 -- X-OriginalArrivalTime: 28 Jun 2007 14:18:25.0328 (UTC) FILETIME=[30C8DB00:01C7B98F] ==============================End of - Headers==============================
} It might be good if the power (real, or setting) and Make/type of } plasma cleaner are specified for these discussions.
A comment from my side to this topic:
Since more than 15 years, I use a Harrick PDC-3XG plasma cleaner, in a similar way as stated by Dale Callaham, of The University of Massachusetts -at- Amherst, in his recent mail on the same topic.
I use it in the HIGH setting, for 15 sec, and get nicely wettable pure carbon films and copper or gold grids, whatever is needed. Excellent reproducability over the last years. Quick and efficient. Just perfect, to my experience. I have the possibility to keep the vacuum at about 0.1 to 1 mbar (reading at a vacuum meter).
I think that the effect lasts for about 1 hour or two, but I did not measure this. I just tell people / students to do it just before applying the samples - say within 10 or 20 min. THis works fine.
I have no connection to this company.
best regards,
Reinhard Rachel ---------------------- PD Dr. Reinhard Rachel Universitaet Regensburg Centre for EM - NWF III - Lehrstuhl fuer Anatomie Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720, 1666(TEM) fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-r.de office: VKL 3.1.29
==============================Original Headers============================== 10, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Thu Jun 28 09:35:49 2007 10, 25 -- Received: from rrzmta2.rz.uni-regensburg.de (rrzmta2.rz.uni-regensburg.de [194.94.155.53]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5SEZnoF015646 10, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 09:35:49 -0500 10, 25 -- Received: from rrzmta2.rz.uni-regensburg.de (localhost [127.0.0.1]) 10, 25 -- by localhost (Postfix) with SMTP id ADA265357 10, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 16:35:54 +0200 (CEST) 10, 25 -- Received: from localhost (donald1.rz.uni-regensburg.de [132.199.4.91]) 10, 25 -- by rrzmta2.rz.uni-regensburg.de (Postfix) with ESMTP id 9B3895417 10, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 16:35:54 +0200 (CEST) 10, 25 -- Received: from dhcp8003.rz.uni-regensburg.de (dhcp8003.rz.uni-regensburg.de [132.199.87.3]) 10, 25 -- by webmail.uni-regensburg.de (IMP) with HTTP 10, 25 -- for {rar04520-at-rrzlic2.uni-regensburg.de} ; Thu, 28 Jun 2007 16:35:48 +0200 10, 25 -- Message-ID: {1183041348.4683c743e11df-at-webmail.uni-regensburg.de} 10, 25 -- Date: Thu, 28 Jun 2007 16:35:48 +0200 10, 25 -- From: reinhard rachel {reinhard.rachel-at-biologie.uni-regensburg.de} 10, 25 -- To: Microscopy-at-microscopy.com 10, 25 -- Subject: [Microscopy] hydrophilic carbon film 10, 25 -- References: {200706281413.l5SEDDUi023898-at-ns.microscopy.com} 10, 25 -- In-Reply-To: {200706281413.l5SEDDUi023898-at-ns.microscopy.com} 10, 25 -- MIME-Version: 1.0 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1 10, 25 -- Content-Transfer-Encoding: 8bit 10, 25 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 10, 25 -- X-Originating-IP: 132.199.87.3 ==============================End of - Headers==============================
I was recently perusing one of the supply catalogs for SEM supplies, and I found a wet-cell capsule that has an "Electron transparent and vacuum tight window." (http://www.emsdiasum.com/microscopy/products/sem/wet/technical.aspx) I was just wondering if anybody has used these, and what kind of results they are getting.
The second part of my question is a bit more abstract. What quality makes something electron transparent, and what materials are electron transparent?
--Justin A. Kraft
==============================Original Headers============================== 3, 26 -- From kraftpiano-at-gmail.com Thu Jun 28 09:50:21 2007 3, 26 -- Received: from nz-out-0506.google.com (nz-out-0506.google.com [64.233.162.224]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5SEoLV7028166 3, 26 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 09:50:21 -0500 3, 26 -- Received: by nz-out-0506.google.com with SMTP id o37so298124nzf 3, 26 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 07:50:20 -0700 (PDT) 3, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 3, 26 -- d=gmail.com; s=beta; 3, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 26 -- b=bt+Htx+pJCmpgBuMqNzbplobSGNCbF2fFry0/DCwqjtAxmCxvQFZ3MXozGBzITWOcm/p2S98khI7IdZJSpSuE/jZIakDr9QyGlDf51pMC5FNghu8ujY2mEnlGxmhZgJoAZESnPgcFzJ4Vg9SAiChK03oy7BXmUVa4jsEfP8g9Oc= 3, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 26 -- d=gmail.com; s=beta; 3, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 26 -- b=T+UdxaTpfOHqtuwLCmbF7bR7MBslXHj/o5CIXX7qqdLRurgNulowHAqE4pWMbyFyrSbVLvHO+ZidsnKsXkQFcHb2GJYVUo0qjWy6c6bnCfNUaSmi3TvoHVVZl47e9lU26MpI6CO9YdipK0Ge4OHiGUVbxUB/2UOEJN/aaJXLvzE= 3, 26 -- Received: by 10.114.176.1 with SMTP id y1mr1591665wae.1183042220137; 3, 26 -- Thu, 28 Jun 2007 07:50:20 -0700 (PDT) 3, 26 -- Received: by 10.114.78.15 with HTTP; Thu, 28 Jun 2007 07:50:20 -0700 (PDT) 3, 26 -- Message-ID: {25e2b0d20706280750v623999a7le6402ab06b6d645-at-mail.gmail.com} 3, 26 -- Date: Thu, 28 Jun 2007 10:50:20 -0400 3, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 26 -- To: microscopy-at-microscopy.com 3, 26 -- Subject: SEM: Wet SEM capsule 3, 26 -- MIME-Version: 1.0 3, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 26 -- Content-Transfer-Encoding: 7bit 3, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
I have not used the capsule, so cannot comment on it. Generally, a material becomes more transparent to electrons as the atomic number / density drops and the material becomes thinner.
The capsule window cannot be completely electron transparent. From what I read (if memory serves) it is not transparent to the low energy electrons used for secondary imaging, but is transparent enough to let a higher energy incident beam and the resultant BSEs to pass.
Same principle as the thin windows on EDS detectors that allow very low energy x-rays to penetrate.
Woody
NW (Woody) White Jr BWXT Services Lynchburg, VA http://www.bwxt.com/operations/semlab.html
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Thursday, June 28, 2007 10:51 AM To: White, Woody N.
I was recently perusing one of the supply catalogs for SEM supplies, and I found a wet-cell capsule that has an "Electron transparent and vacuum tight window." (http://www.emsdiasum.com/microscopy/products/sem/wet/technical.aspx) I was just wondering if anybody has used these, and what kind of results they are getting.
The second part of my question is a bit more abstract. What quality makes something electron transparent, and what materials are electron transparent?
--Justin A. Kraft
==============================Original Headers============================== 3, 26 -- From kraftpiano-at-gmail.com Thu Jun 28 09:50:21 2007 3, 26 -- Received: from nz-out-0506.google.com (nz-out-0506.google.com [64.233.162.224]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5SEoLV7028166 3, 26 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 09:50:21 -0500 3, 26 -- Received: by nz-out-0506.google.com with SMTP id o37so298124nzf 3, 26 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 07:50:20 -0700 (PDT) 3, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 3, 26 -- d=gmail.com; s=beta; 3, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject: mime-version:content-type:content-transfer-encoding:content-disposition; 3, 26 -- b=bt+Htx+pJCmpgBuMqNzbplobSGNCbF2fFry0/DCwqjtAxmCxvQFZ3MXozGBzITWOcm/p2S 98khI7IdZJSpSuE/jZIakDr9QyGlDf51pMC5FNghu8ujY2mEnlGxmhZgJoAZESnPgcFzJ4Vg 9SAiChK03oy7BXmUVa4jsEfP8g9Oc= 3, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 26 -- d=gmail.com; s=beta; 3, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:con tent-transfer-encoding:content-disposition; 3, 26 -- b=T+UdxaTpfOHqtuwLCmbF7bR7MBslXHj/o5CIXX7qqdLRurgNulowHAqE4pWMbyFyrSbVLv HO+ZidsnKsXkQFcHb2GJYVUo0qjWy6c6bnCfNUaSmi3TvoHVVZl47e9lU26MpI6CO9YdipK0 Ge4OHiGUVbxUB/2UOEJN/aaJXLvzE= 3, 26 -- Received: by 10.114.176.1 with SMTP id y1mr1591665wae.1183042220137; 3, 26 -- Thu, 28 Jun 2007 07:50:20 -0700 (PDT) 3, 26 -- Received: by 10.114.78.15 with HTTP; Thu, 28 Jun 2007 07:50:20 -0700 (PDT) 3, 26 -- Message-ID: {25e2b0d20706280750v623999a7le6402ab06b6d645-at-mail.gmail.com} 3, 26 -- Date: Thu, 28 Jun 2007 10:50:20 -0400 3, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 26 -- To: microscopy-at-microscopy.com 3, 26 -- Subject: SEM: Wet SEM capsule 3, 26 -- MIME-Version: 1.0 3, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 26 -- Content-Transfer-Encoding: 7bit 3, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 15, 28 -- From nwwhite-at-bwxt.com Thu Jun 28 10:11:41 2007 15, 28 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5SFBefc008820 15, 28 -- for {microscopy-at-msa.microscopy.com} ; Thu, 28 Jun 2007 10:11:40 -0500 15, 28 -- Received: from ([131.184.13.224]) 15, 28 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.5256717; 15, 28 -- Thu, 28 Jun 2007 11:11:15 -0400 15, 28 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); 15, 28 -- Thu, 28 Jun 2007 11:11:15 -0400 15, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 15, 28 -- Content-class: urn:content-classes:message 15, 28 -- MIME-Version: 1.0 15, 28 -- Content-Type: text/plain; 15, 28 -- charset="us-ascii" 15, 28 -- Subject: RE: [Microscopy] SEM: Wet SEM capsule 15, 28 -- Date: Thu, 28 Jun 2007 11:11:15 -0400 15, 28 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB7F8794C-at-BWXSPO01.BWXS.BWXTECH.NET} 15, 28 -- In-Reply-To: {200706281450.l5SEoiG1028942-at-ns.microscopy.com} 15, 28 -- X-MS-Has-Attach: 15, 28 -- X-MS-TNEF-Correlator: 15, 28 -- Thread-Topic: [Microscopy] SEM: Wet SEM capsule 15, 28 -- Thread-Index: Ace5k8FE/Q4gHJOMQEKioCAlJD667QAAdJcg 15, 28 -- References: {200706281450.l5SEoiG1028942-at-ns.microscopy.com} 15, 28 -- From: "White, Woody N." {NWWhite-at-bwxt.com} 15, 28 -- To: "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 15, 28 -- X-OriginalArrivalTime: 28 Jun 2007 15:11:15.0378 (UTC) FILETIME=[92481920:01C7B996] 15, 28 -- Content-Transfer-Encoding: 8bit 15, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5SFBefc008820 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both LyudmilaSolovyeva-at-eaton.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Commercial vendor for SEM/EDX service
Question: We use in our company LEO 1455 Variable Pressure SEM with Ixford INCA EDX system. We are looking for alternative Service contract for SEM/EDX. Could you, please, provide a list of vendors whi is available to do this job.
Thank you.
Lyuda Solovyeva Lead Engineer - Eaton Innovation Center Southfield, MI Ph. 248-226-7179 Fax. 248-226-7165
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both metzger-at-geosun.sjsu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: metzger-at-geosun.sjsu.edu Name: Ellen Metzger
Organization: San Jose State University
Title-Subject: [Filtered] Electron microprobe - carbon coater
Question: We are in the process of setting up a probe lab and are in search of a carbon coater. I'd appreciate advice about carbon fiber versus rod and turbo pumped versus non-turbo for microprobe analysis of polished thin sections.
Our Electron Microprobe Lab has a JEOL JEE-400 Vacuum Evaporator, mid-1990s vintage. It uses sharpened carbon rods and has a compound rotary-diffusion vacuum system. The vacuum system valves are all manual (no electronic automation), and the threat of cleaning burnt diffusion-pump oil instills a healthy sense of fear in students.
A turbo pump will increase the cost of your coater by about 50% (last time that I priced them), and it will be faster but not necessarily any better. Diffusion pumps are hardy, and after 12 years or so, I think we're on our third rotary pump (although we probably could have repaired the broken ones if we were so inclined).
In our experience with samples coated in other facilities, the fiber- type carbon coaters generally do not seem to apply as smooth a coat as our rod-type coater. I usually insist that samples going in our microprobe be coating in our lab, not elsewhere. This, though, is based only on anecdotal evidence and our experiences, not any systemic study.
Feel free to email me directly with any other questions, and you can sign up for my microprobe-based listserver...
http://probelab.geo.umn.edu/listserver.html
... where you can seek advice from our microprobe users and facility managers.
Best, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu Personal Website: http://umn.edu/~frah0010
On Jun 28, 2007, at 7:40 PM, metzger-at-geosun.sjsu.edu wrote: } } Email: metzger-at-geosun.sjsu.edu } Name: Ellen Metzger } } Organization: San Jose State University } } Title-Subject: [Filtered] Electron microprobe - carbon coater } } Question: We are in the process of setting up a probe lab and are } in search of a carbon coater. I'd appreciate advice about carbon } fiber versus rod and turbo pumped versus non-turbo for microprobe } analysis of polished thin sections.
==============================Original Headers============================== 12, 19 -- From frah0010-at-umn.edu Thu Jun 28 20:17:56 2007 12, 19 -- Received: from mta-m2.tc.umn.edu (mta-m2.tc.umn.edu [134.84.119.106]) 12, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5T1HuOa003554 12, 19 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 20:17:56 -0500 12, 19 -- Received: from [10.0.1.3] (c-75-72-182-252.hsd1.mn.comcast.net [75.72.182.252]) 12, 19 -- by mta-m2.tc.umn.edu (UMN smtpd) with ESMTP 12, 19 -- Thu, 28 Jun 2007 20:17:56 -0500 (CDT) 12, 19 -- X-Umn-Remote-Mta: [N] c-75-72-182-252.hsd1.mn.comcast.net [75.72.182.252] #+TS+AU+HN 12, 19 -- Mime-Version: 1.0 (Apple Message framework v752.2) 12, 19 -- In-Reply-To: {200706290040.l5T0eTWT030322-at-ns.microscopy.com} 12, 19 -- References: {200706290040.l5T0eTWT030322-at-ns.microscopy.com} 12, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 12, 19 -- Message-Id: {B91A9530-C8DD-4DB2-8EF5-99E78FCB0ED5-at-umn.edu} 12, 19 -- Content-Transfer-Encoding: 7bit 12, 19 -- From: Ellery Frahm {frah0010-at-umn.edu} 12, 19 -- Subject: Re: [Microscopy] viaWWW: Electron microprobe - carbon coater 12, 19 -- Date: Thu, 28 Jun 2007 20:17:52 -0500 12, 19 -- To: metzger-at-geosun.sjsu.edu, microscopy-at-microscopy.com 12, 19 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
In TEM the HT value is usually expressed in kV whereas for SEM it is usually expressed in keV. Being biologist, I don't easily comprehend the difference between the 2 units. In TEM the HT value represents the real tension between the anode and the cathode. I expect that is the same in SEM, so why add an "e" in-between? Does it change anything, except the unecological use of extra amount of ink?
Best regards,
Stephane
____________________________________________________________________________________ Got a little couch potato? Check out fun summer activities for kids. http://search.yahoo.com/search?fr=oni_on_mail&p=summer+activities+for+kids&cs=bz
==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Fri Jun 29 04:26:23 2007 6, 19 -- Received: from web37414.mail.mud.yahoo.com (web37414.mail.mud.yahoo.com [209.191.91.146]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5T9QNvX024455 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 04:26:23 -0500 6, 19 -- Received: (qmail 81107 invoked by uid 60001); 29 Jun 2007 09:26:22 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 6, 19 -- b=6o0V/Xaz+RSjMhL2R8JdTrRTAtd9i9xWnBxMHS15hK5vcvL0SThdV8If4BauOPXTmaV+R6BnKM2WFC9hhvy5svD1fQhMcuZDsZlvqCDxVAQTQ3ZiKmt2tdLAYMDPqbChmFiomE2llP6FVg85keil0n4jYIeWK4K/Nk+twcL5MHU=; 6, 19 -- X-YMail-OSG: cO7p8wEVM1nc9aKShOLzmFIsVU_Mew_ahLdGCst0SVjn3UAL7mPKOuPp3LDK4vOzPlJ_DgcjgruPTks0MamXWHSAiwgg6hhk2GzKPB2IGdsZXSDXxbjxRsgpVxUU2w-- 6, 19 -- Received: from [80.122.101.102] by web37414.mail.mud.yahoo.com via HTTP; Fri, 29 Jun 2007 02:26:22 PDT 6, 19 -- Date: Fri, 29 Jun 2007 02:26:22 -0700 (PDT) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 -- Subject: kV or keV? 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- Message-ID: {879217.80366.qm-at-web37414.mail.mud.yahoo.com} ==============================End of - Headers==============================
} In TEM the HT value represents the real tension between the } anode and the cathode. I expect that is the same in SEM, so } why add an "e" in-between? Does it change anything, ...
Tecnically, "kV" is a unit of potential energy, while "keV" is a unit of kinetic energy because 'e' is supposed to include the mass of an electron. In reality, we use them both interchangably.
HTH & Genuinely, Michael Shaffer :o)
SEM/MLA Research Coordinator http://www.mun.ca/creait/maf/
Inco Innovation Centre Memorial University St. John's, Newfoundland
==============================Original Headers============================== 8, 21 -- From michael-at-shaffer.net Fri Jun 29 04:44:43 2007 8, 21 -- Received: from n034.sc1.he.tucows.com (smtpout1471.sc1.he.tucows.com [64.97.157.171]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5T9igaq003876 8, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 04:44:43 -0500 8, 21 -- Received: from rarewolf (205.251.83.78) by n034.sc1.he.tucows.com (7.2.069.1) (authenticated as michael-at-shaffer.net) 8, 21 -- id 467DB62F001123C8; Fri, 29 Jun 2007 09:44:42 +0000 8, 21 -- From: "michael shaffer" {michael-at-shaffer.net} 8, 21 -- To: {nizets2-at-yahoo.com} 8, 21 -- Cc: "MSA Microscopy list" {Microscopy-at-microscopy.com} 8, 21 -- References: {200706290926.l5T9Qpqd025115-at-ns.microscopy.com} 8, 21 -- Subject: RE: [Microscopy] kV or keV? 8, 21 -- Date: Fri, 29 Jun 2007 07:14:27 -0230 8, 21 -- Message-ID: {00ef01c7ba32$164a07b0$4701a8c0-at-rarewolf} 8, 21 -- MIME-Version: 1.0 8, 21 -- Content-Type: text/plain; 8, 21 -- charset="US-ASCII" 8, 21 -- Content-Transfer-Encoding: 7bit 8, 21 -- X-Mailer: Microsoft Office Outlook 11 8, 21 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 8, 21 -- Thread-index: Ace6LwCfDMcf55FMTq+Bin9a7pq8MAAAnJEQ 8, 21 -- In-Reply-To: {200706290926.l5T9Qpqd025115-at-ns.microscopy.com} ==============================End of - Headers==============================
HT is a voltage and therefore always has the unit V(olt) or kV for kilo Volt. I recommend any SEMer who thinks otherwise to take a basic physics course.
keV stands for kilo electronvolt, which is a (non-SI) energy-unit. That's something completely different. To get from voltage to energy you have to multiply with the charge of the particle traveling through the potential-difference (or voltage). 1 eV is the kinetic energy of an electron that has been accelerated by a voltage of 1 V. Just to make things clear: This is independent of the electron's mass. It only depends on charge and voltage!
Hope that helps,
Philip
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: 29 June 2007 11:30 To: Philip.Koeck-at-biosci.ki.se
Dear listers,
In TEM the HT value is usually expressed in kV whereas for SEM it is usually expressed in keV. Being biologist, I don't easily comprehend the difference between the 2 units. In TEM the HT value represents the real tension between the anode and the cathode. I expect that is the same in SEM, so why add an "e" in-between? Does it change anything, except the unecological use of extra amount of ink?
Best regards,
Stephane
==============================Original Headers============================== 16, 25 -- From Philip.Koeck-at-biosci.ki.se Fri Jun 29 05:55:10 2007 16, 25 -- Received: from smtp.biosci.ki.se (smtp.biosci.ki.se [130.237.109.196]) 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TAtAjN016856 16, 25 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 05:55:10 -0500 16, 25 -- Received: from smtp.biosci.ki.se (localhost [127.0.0.1]) 16, 25 -- by localhost (Postfix) with ESMTP id CC2994FF15; 16, 25 -- Fri, 29 Jun 2007 12:55:09 +0200 (CEST) 16, 25 -- Received: from whale.csb.ki.se (whale.csb.ki.se [130.237.109.8]) 16, 25 -- by smtp.biosci.ki.se (Postfix) with SMTP id BDBD54FEEE; 16, 25 -- Fri, 29 Jun 2007 12:55:09 +0200 (CEST) 16, 25 -- Received: from rapana.csb.ki.se by whale.csb.ki.se (5.65v4.0/1.1.10.5/03Feb98-0237PM) 16, 25 -- id AA00580; Fri, 29 Jun 2007 12:55:09 +0200 16, 25 -- Message-Id: {10706291055.AA00580-at-whale.csb.ki.se} 16, 25 -- From: "Philip Koeck" {Philip.Koeck-at-biosci.ki.se} 16, 25 -- To: {nizets2-at-yahoo.com} , {microscopy-at-microscopy.com} 16, 25 -- Subject: RE: [Microscopy] kV or keV? 16, 25 -- Date: Fri, 29 Jun 2007 12:56:05 +0200 16, 25 -- Mime-Version: 1.0 16, 25 -- Content-Type: text/plain; 16, 25 -- charset="US-ASCII" 16, 25 -- Content-Transfer-Encoding: 7bit 16, 25 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353 16, 25 -- X-Mimeole: Produced By Microsoft MimeOLE V6.00.2900.3138 16, 25 -- In-Reply-To: {200706290929.l5T9TY7Y028956-at-ns.microscopy.com} 16, 25 -- Thread-Index: Ace6MAIdcfl1dJRvTsi0S83vO1mkLAACa2Og ==============================End of - Headers==============================
} Technically, "kV" is a unit of potential energy, while "keV" is a } unit of } kinetic energy because 'e' is supposed to include the mass of an } electron. } In reality, we use them both interchangeably.
Except that students in my microprobe course who try to use these terms interchangeably loose points. The same happens in the TEM course taught at our university. It is the same difference between potential and potential energy -- subtle but non-trivial.
Best, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu Personal Website: http://umn.edu/~frah0010
==============================Original Headers============================== 5, 19 -- From frah0010-at-umn.edu Fri Jun 29 07:21:58 2007 5, 19 -- Received: from mta-a2.tc.umn.edu (mta-a2.tc.umn.edu [134.84.119.206]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TCLwFq030460 5, 19 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 07:21:58 -0500 5, 19 -- Received: from [10.0.1.3] (c-75-72-182-252.hsd1.mn.comcast.net [75.72.182.252]) 5, 19 -- by mta-a2.tc.umn.edu (UMN smtpd) with ESMTP 5, 19 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 07:21:58 -0500 (CDT) 5, 19 -- X-Umn-Remote-Mta: [N] c-75-72-182-252.hsd1.mn.comcast.net [75.72.182.252] #+TS+AU+HN 5, 19 -- Mime-Version: 1.0 (Apple Message framework v752.2) 5, 19 -- In-Reply-To: {200706290948.l5T9mxYM013024-at-ns.microscopy.com} 5, 19 -- References: {200706290948.l5T9mxYM013024-at-ns.microscopy.com} 5, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 19 -- Message-Id: {99ADBC28-390F-4EA4-BAC1-B1EDEAC91767-at-umn.edu} 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- From: Ellery Frahm {frah0010-at-umn.edu} 5, 19 -- Subject: Re: [Microscopy] RE: kV or keV? 5, 19 -- Date: Fri, 29 Jun 2007 07:21:56 -0500 5, 19 -- To: microscopy-at-microscopy.com 5, 19 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
For the upcoming MSA meeting, is there a schedule yet as to specific symposia and tutorial sessions? I am trying to decide on which days to attend.
Dotty Sorenson
Dorothy Sorenson Microscopy and Image-analysis Laboratory Department of Cell and Developmental Biology University Of Michigan Medical School A807 BSRB 109 Zina Pitcher Place Ann Arbor, MI 48109-2200 (734)763-1170 FAX (734)763-1166
==============================Original Headers============================== 5, 16 -- From dsoren-at-umich.edu Fri Jun 29 08:05:11 2007 5, 16 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.161]) 5, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TD5Bpq010592 5, 16 -- for {microscopy-at-msa.microscopy.com} ; Fri, 29 Jun 2007 08:05:11 -0500 5, 16 -- Received: FROM [10.21.129.180] (host-18.subnet-17.med.umich.edu [141.214.17.18]) 5, 16 -- BY tombraider.mr.itd.umich.edu ID 4685034A.3E8F0.32569 ; 5, 16 -- 29 Jun 2007 09:04:10 -0400 5, 16 -- Mime-Version: 1.0 (Apple Message framework v752.2) 5, 16 -- Content-Transfer-Encoding: 7bit 5, 16 -- Message-Id: {5F4AB454-9322-452E-9490-9D8EDEA33F99-at-umich.edu} 5, 16 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 16 -- To: microscopy-at-msa.microscopy.com 5, 16 -- From: Dorothy Sorenson {dsoren-at-umich.edu} 5, 16 -- Subject: MSA schedule 5, 16 -- Date: Fri, 29 Jun 2007 09:00:41 -0400 5, 16 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
I was wondering if anyone on the listserve has any experience with the Agilent 5500 series AFMs? I am especially interested to know how it compares with the Veeco Multimode and/or Dimension...
Thank you,
Andy Bowling
==============================Original Headers============================== 5, 30 -- From Andrew.Bowling-at-ARS.USDA.GOV Fri Jun 29 08:23:48 2007 5, 30 -- Received: from messagescreen4.ars.usda.gov (messagescreen4.ars.usda.gov [199.133.180.151]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TDNmje022509 5, 30 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 08:23:48 -0500 5, 30 -- Received: from CO-MAILBH-02.ARSNET.ARS.USDA.GOV ([199.133.183.227]) 5, 30 -- by messagescreen4.ars.usda.gov (8.13.8/8.13.8) with ESMTP id l5TDNlbT021550 5, 30 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 08:23:48 -0500 5, 30 -- Received: from CO-MAIL-03.ARSNET.ARS.USDA.GOV ([10.100.2.202]) by CO-MAILBH-02.ARSNET.ARS.USDA.GOV with Microsoft SMTPSVC(6.0.3790.3959); 5, 30 -- Fri, 29 Jun 2007 07:23:47 -0600 5, 30 -- Content-class: urn:content-classes:message 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Type: text/plain; 5, 30 -- charset="us-ascii" 5, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 30 -- Subject: AFM opinions 5, 30 -- Date: Fri, 29 Jun 2007 07:23:47 -0600 5, 30 -- Message-ID: {8017F94146BF634DA9414E4B9088525B0DE1C5-at-CO-MAIL-03.ARSNET.ARS.USDA.GOV} 5, 30 -- X-MS-Has-Attach: 5, 30 -- X-MS-TNEF-Correlator: 5, 30 -- Thread-Topic: AFM opinions 5, 30 -- Thread-Index: Ace6WN0/1BkCVW6+QPmII/TcfTv+Ww== 5, 30 -- From: "Bowling, Andrew" {Andrew.Bowling-at-ARS.USDA.GOV} 5, 30 -- To: {microscopy-at-microscopy.com} 5, 30 -- X-OriginalArrivalTime: 29 Jun 2007 13:23:47.0502 (UTC) FILETIME=[B9760CE0:01C7BA50] 5, 30 -- X-MessageScreenMessageID: 1183123428.47976.1254.627261455 5, 30 -- X-MessageScreenContentScore: Score of 0 assigned to Content 5, 30 -- X-MessageScreenUCEScore: Score of 0 assigned to UCE 5, 30 -- X-MessageScreen: Analyzed by IntelliReach MessageScreen(tm) 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5TDNmje022509 ==============================End of - Headers==============================
We recently had a 10L LN2 dewar lose vacuum and have had no luck whatsoever finding someone who could pump it down at a reasonable (i.e., less than replacement) price. The dewar manufacturer said that they do not (will not?) supply connectors for vacuum hoses so that we could connect it to one of our pumps. Our own physics shop says that they haven't pumped one for 15 years and it would cost hundreds of dollars and lots of LN2 to do this.
Bottom line: buy a new dewar for $500+.
Can someone explain why this is such a huge problem? There is a vacuum port on the dewar, and I assume it is there for a reason. I have seen vacuum restored to EDS LN2 reservoirs by simply hooking them up to a pump for basically the cost of the electricity to run the pump. Where can we find connectors? Someone obviously makes them, but it seems to be a well-kept secret.
Please reply to the list, since I expect I'm not the only one wandering around in a vacuum and losing my cool.
Thanks much and Happy Fourth of July!
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 9, 23 -- From TindallR-at-missouri.edu Fri Jun 29 09:06:24 2007 9, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TE6Obv002564 9, 23 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 09:06:24 -0500 9, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 9, 23 -- Fri, 29 Jun 2007 09:06:24 -0500 9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 23 -- Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="us-ascii" 9, 23 -- Subject: LN2 Dewars failing 9, 23 -- Date: Fri, 29 Jun 2007 09:06:24 -0500 9, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BB5D-at-UM-XMAIL08.um.umsystem.edu} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: LN2 Dewars failing 9, 23 -- Thread-Index: Ace6VreGPz/ecE35QRajl6Cp8g1xPA== 9, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 9, 23 -- To: {microscopy-at-microscopy.com} 9, 23 -- X-OriginalArrivalTime: 29 Jun 2007 14:06:24.0668 (UTC) FILETIME=[ADA69DC0:01C7BA56] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5TE6Obv002564 ==============================End of - Headers==============================
On 29 Jun 2007 at 8:06, dsoren-at-umich.edu wrote:
} } For the upcoming MSA meeting, is there a schedule yet as to specific } symposia and tutorial sessions? I am trying to decide on which days } to attend. } } Dotty Sorenson } } Dorothy Sorenson } Microscopy and Image-analysis Laboratory } Department of Cell and Developmental Biology } University Of Michigan Medical School } A807 BSRB } 109 Zina Pitcher Place } Ann Arbor, MI 48109-2200 } (734)763-1170 } FAX (734)763-1166 } } } } ==============================Original Headers============================== } 5, 16 -- From dsoren-at-umich.edu Fri Jun 29 08:05:11 2007 } 5, 16 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.161]) } 5, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TD5Bpq010592 } 5, 16 -- for {microscopy-at-msa.microscopy.com} ; Fri, 29 Jun 2007 08:05:11 -0500 } 5, 16 -- Received: FROM [10.21.129.180] (host-18.subnet-17.med.umich.edu [141.214.17.18]) } 5, 16 -- BY tombraider.mr.itd.umich.edu ID 4685034A.3E8F0.32569 ; } 5, 16 -- 29 Jun 2007 09:04:10 -0400 } 5, 16 -- Mime-Version: 1.0 (Apple Message framework v752.2) } 5, 16 -- Content-Transfer-Encoding: 7bit } 5, 16 -- Message-Id: {5F4AB454-9322-452E-9490-9D8EDEA33F99-at-umich.edu} } 5, 16 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed } 5, 16 -- To: microscopy-at-msa.microscopy.com } 5, 16 -- From: Dorothy Sorenson {dsoren-at-umich.edu} } 5, 16 -- Subject: MSA schedule } 5, 16 -- Date: Fri, 29 Jun 2007 09:00:41 -0400 } 5, 16 -- X-Mailer: Apple Mail (2.752.2) } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 8, 24 -- From edelmare-at-muohio.edu Fri Jun 29 09:19:08 2007 8, 24 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TEJ740014337 8, 24 -- for {microscopy-at-Microscopy.com} ; Fri, 29 Jun 2007 09:19:07 -0500 8, 24 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 8, 24 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l5TEJ722002889; 8, 24 -- Fri, 29 Jun 2007 10:19:07 -0400 8, 24 -- Received: from [192.168.1.23] ([134.53.14.105]) 8, 24 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l5TEJ6RX024338; 8, 24 -- Fri, 29 Jun 2007 10:19:06 -0400 8, 24 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 8, 24 -- To: dsoren-at-umich.edu, microscopy-at-Microscopy.com 8, 24 -- Date: Fri, 29 Jun 2007 10:19:07 -0400 8, 24 -- MIME-Version: 1.0 8, 24 -- Subject: Re: [Microscopy] MSA schedule 8, 24 -- Message-ID: {4684DC9B.6797.E0C1B3F-at-edelmare.muohio.edu} 8, 24 -- Priority: normal 8, 24 -- In-reply-to: {200706291306.l5TD60H5011824-at-ns.microscopy.com} 8, 24 -- References: {200706291306.l5TD60H5011824-at-ns.microscopy.com} 8, 24 -- X-mailer: Pegasus Mail for Windows (4.41) 8, 24 -- Content-type: text/plain; charset=US-ASCII 8, 24 -- Content-transfer-encoding: 7BIT 8, 24 -- Content-description: Mail message body 8, 24 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
Turbo pumped systems pump faster. They pump to a lower pressure than rotary pump only systems (i.e. table top models). Lower pressure equals finer coating grain size.
Fiber (rope, cord, etc.) is a quick and easy carbon coat, and can get away with higher pressures, but it is rougher, and can leave "chunks" of fiber on sample. Not particularly controlable as to thickness (use more fibers vs fewer fibers).
Rods systems tend to be finer coating, more controlable as to "thickness" (evaporate more or less of the rod).
If you are thinking about EBSD work in the future, and might wish to use a very thin carbon coat (yes, before someone yells out, it will obsure some of the signal). The control of the rod systems is needed.
I have not compared fiber vs rod for electron microprobe work on polished sections. We use a full blown denton vacuum evaporator (very fine, very versitile, very slow), and I really wish I could get a turbo pumped table top model to easy speed for most SEM work. . .
On 28 Jun 2007 at 19:33, metzger-at-geosun.sjsu.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both metzger-at-geosun.sjsu.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: metzger-at-geosun.sjsu.edu } Name: Ellen Metzger } } Organization: San Jose State University } } Title-Subject: [Filtered] Electron microprobe - carbon coater } } Question: We are in the process of setting up a probe lab and are in } search of a carbon coater. I'd appreciate advice about carbon fiber } versus rod and turbo pumped versus non-turbo for microprobe analysis } of polished thin sections. } } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Thu Jun 28 19:33:12 2007 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5T0XA4N012579 } 8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 19:33:12 -0500 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240804c2aa03ba31cc-at-[206.69.208.22]} } 8, 11 -- Date: Thu, 28 Jun 2007 19:33:09 -0500 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: metzger-at-geosun.sjsu.edu (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: Electron microprobe - carbon coater } 8, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
==============================Original Headers============================== 13, 25 -- From edelmare-at-muohio.edu Fri Jun 29 09:33:34 2007 13, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TEXY0b026093 13, 25 -- for {microscopy-at-Microscopy.com} ; Fri, 29 Jun 2007 09:33:34 -0500 13, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 13, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l5TEXWYD015984; 13, 25 -- Fri, 29 Jun 2007 10:33:32 -0400 13, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 13, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l5TEXWYU027030; 13, 25 -- Fri, 29 Jun 2007 10:33:32 -0400 13, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 13, 25 -- To: metzger-at-geosun.sjsu.edu 13, 25 -- Date: Fri, 29 Jun 2007 10:33:32 -0400 13, 25 -- MIME-Version: 1.0 13, 25 -- Subject: Re: [Microscopy] viaWWW: Electron microprobe - carbon coater 13, 25 -- CC: microscopy-at-Microscopy.com 13, 25 -- Message-ID: {4684DFFC.1900.E19503E-at-edelmare.muohio.edu} 13, 25 -- Priority: normal 13, 25 -- In-reply-to: {200706290033.l5T0Xu9c014602-at-ns.microscopy.com} 13, 25 -- References: {200706290033.l5T0Xu9c014602-at-ns.microscopy.com} 13, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 13, 25 -- Content-type: text/plain; charset=US-ASCII 13, 25 -- Content-transfer-encoding: 7BIT 13, 25 -- Content-description: Mail message body 13, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 ==============================End of - Headers==============================
"kV" is kilovolts, an electrical potential (SI units). It is the electric potential between the anode and cathode.
"keV" is kilo-electron-volts, a unit of energy (doesn't matter if kinetic or potential). It represents the energy of a particle having a unit (electron) charge in a 1 kV field (or its kinetic energy after passing through a 1 kV field). Although not strictly SI, it is considered a "unit accepted for use with SI"* It has nothing to do with the particle's mass.
One can use either to describe the operating conditions of your SEM or TEM interchangeably, since we're always accelerating electrons.
For EDS or EELS spectra, the unit is always keV or eV (unless an SI purist prefers zeptojoules) since in the case of EDS one is referring to the energy of an x-ray (chargeless and massless!), and in EELS one is measuring **energy transfer** by an electron.
* See R. A. Nelson, "Guide for Metric Practice," Physics Today BG13 (1997).
"Before I came here I was confused about this subject. Having listened to your lecture I am still confused, but on a higher level." - Enrico Fermi
Jeffrey A. Fortner, Ph.D. Chemical Engineering Division Argonne National Laboratory Argonne, IL 60439 fortner(at)cmt.anl.gov
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Friday, June 29, 2007 4:27 AM To: Fortner, Jeffrey A.
Dear listers,
In TEM the HT value is usually expressed in kV whereas for SEM it is usually expressed in keV. Being biologist, I don't easily comprehend the difference between the 2 units. In TEM the HT value represents the real tension between the anode and the cathode. I expect that is the same in SEM, so why add an "e" in-between? Does it change anything, except the unecological use of extra amount of ink?
Best regards,
Stephane
________________________________________________________________________ ____________ Got a little couch potato? Check out fun summer activities for kids. http://search.yahoo.com/search?fr=oni_on_mail&p=summer+activities+for+ki ds&cs=bz
==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Fri Jun 29 04:26:23 2007 6, 19 -- Received: from web37414.mail.mud.yahoo.com (web37414.mail.mud.yahoo.com [209.191.91.146]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5T9QNvX024455 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 04:26:23 -0500 6, 19 -- Received: (qmail 81107 invoked by uid 60001); 29 Jun 2007 09:26:22 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Co ntent-Transfer-Encoding:Message-ID; 6, 19 -- b=6o0V/Xaz+RSjMhL2R8JdTrRTAtd9i9xWnBxMHS15hK5vcvL0SThdV8If4BauOPXTmaV+R6 BnKM2WFC9hhvy5svD1fQhMcuZDsZlvqCDxVAQTQ3ZiKmt2tdLAYMDPqbChmFiomE2llP6FVg 85keil0n4jYIeWK4K/Nk+twcL5MHU=; 6, 19 -- X-YMail-OSG: cO7p8wEVM1nc9aKShOLzmFIsVU_Mew_ahLdGCst0SVjn3UAL7mPKOuPp3LDK4vOzPlJ_Dgcj gruPTks0MamXWHSAiwgg6hhk2GzKPB2IGdsZXSDXxbjxRsgpVxUU2w-- 6, 19 -- Received: from [80.122.101.102] by web37414.mail.mud.yahoo.com via HTTP; Fri, 29 Jun 2007 02:26:22 PDT 6, 19 -- Date: Fri, 29 Jun 2007 02:26:22 -0700 (PDT) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 -- Subject: kV or keV? 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- Message-ID: {879217.80366.qm-at-web37414.mail.mud.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 23, 23 -- From fortner-at-cmt.anl.gov Fri Jun 29 09:47:50 2007 23, 23 -- Received: from cmtmail.cmt.anl.gov (CMTMAIL.cmt.anl.gov [146.139.148.212]) 23, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TElnn4006974 23, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 09:47:50 -0500 23, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 23, 23 -- Content-class: urn:content-classes:message 23, 23 -- MIME-Version: 1.0 23, 23 -- Content-Type: text/plain; 23, 23 -- charset="us-ascii" 23, 23 -- Subject: RE: [Microscopy] kV or keV? 23, 23 -- Date: Fri, 29 Jun 2007 09:47:48 -0500 23, 23 -- Message-ID: {661C1AA445464649B30D32151A7C34FA043C7DEE-at-CMTMAIL.cmt.anl.gov} 23, 23 -- In-Reply-To: {200706290927.l5T9RDE5025673-at-ns.microscopy.com} 23, 23 -- X-MS-Has-Attach: 23, 23 -- X-MS-TNEF-Correlator: 23, 23 -- Thread-Topic: [Microscopy] kV or keV? 23, 23 -- Thread-Index: Ace6L7L/rkLJ0aC5SA6Y1DpOvncfKwAKk7aA 23, 23 -- References: {200706290927.l5T9RDE5025673-at-ns.microscopy.com} 23, 23 -- From: "Fortner, Jeffrey A." {fortner-at-cmt.anl.gov} 23, 23 -- To: {nizets2-at-yahoo.com} , {microscopy-at-ns.microscopy.com} , 23, 23 -- {Microscopy-at-microscopy.com} 23, 23 -- Content-Transfer-Encoding: 8bit 23, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5TElnn4006974 ==============================End of - Headers==============================
"kV" is kilovolts, an electrical potential (SI units). It is the electric potential between the anode and cathode.
"keV" is kilo-electron-volts, a unit of energy (doesn't matter if kinetic or potential). It represents the energy of a particle having a unit (electron) charge in a 1 kV field (or its kinetic energy after passing through a 1 kV field). Although not strictly SI, it is considered a "unit accepted for use with SI"* It has nothing to do with the particle's mass.
One can use either to describe the operating conditions of your SEM or TEM interchangeably, since we're always accelerating electrons.
For EDS or EELS spectra, the unit is always keV or eV (unless an SI purist prefers zeptojoules) since in the case of EDS one is referring to the energy of an x-ray (chargeless and massless!), and in EELS one is measuring **energy transfer** by an electron.
* See R. A. Nelson, "Guide for Metric Practice," Physics Today BG13 (1997).
"Before I came here I was confused about this subject. Having listened to your lecture I am still confused, but on a higher level." - Enrico Fermi
Jeffrey A. Fortner, Ph.D. Chemical Engineering Division Argonne National Laboratory Argonne, IL 60439 fortner(at)cmt.anl.gov
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Friday, June 29, 2007 4:27 AM To: Fortner, Jeffrey A.
Dear listers,
In TEM the HT value is usually expressed in kV whereas for SEM it is usually expressed in keV. Being biologist, I don't easily comprehend the difference between the 2 units. In TEM the HT value represents the real tension between the anode and the cathode. I expect that is the same in SEM, so why add an "e" in-between? Does it change anything, except the unecological use of extra amount of ink?
Best regards,
Stephane
________________________________________________________________________ ____________ Got a little couch potato? Check out fun summer activities for kids. http://search.yahoo.com/search?fr=oni_on_mail&p=summer+activities+for+ki ds&cs=bz
==============================Original Headers============================== 6, 19 -- From nizets2-at-yahoo.com Fri Jun 29 04:26:23 2007 6, 19 -- Received: from web37414.mail.mud.yahoo.com (web37414.mail.mud.yahoo.com [209.191.91.146]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5T9QNvX024455 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 04:26:23 -0500 6, 19 -- Received: (qmail 81107 invoked by uid 60001); 29 Jun 2007 09:26:22 -0000 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 19 -- s=s1024; d=yahoo.com; 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Co ntent-Transfer-Encoding:Message-ID; 6, 19 -- b=6o0V/Xaz+RSjMhL2R8JdTrRTAtd9i9xWnBxMHS15hK5vcvL0SThdV8If4BauOPXTmaV+R6 BnKM2WFC9hhvy5svD1fQhMcuZDsZlvqCDxVAQTQ3ZiKmt2tdLAYMDPqbChmFiomE2llP6FVg 85keil0n4jYIeWK4K/Nk+twcL5MHU=; 6, 19 -- X-YMail-OSG: cO7p8wEVM1nc9aKShOLzmFIsVU_Mew_ahLdGCst0SVjn3UAL7mPKOuPp3LDK4vOzPlJ_Dgcj gruPTks0MamXWHSAiwgg6hhk2GzKPB2IGdsZXSDXxbjxRsgpVxUU2w-- 6, 19 -- Received: from [80.122.101.102] by web37414.mail.mud.yahoo.com via HTTP; Fri, 29 Jun 2007 02:26:22 PDT 6, 19 -- Date: Fri, 29 Jun 2007 02:26:22 -0700 (PDT) 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 19 -- Subject: kV or keV? 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- Message-ID: {879217.80366.qm-at-web37414.mail.mud.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 23, 23 -- From fortner-at-cmt.anl.gov Fri Jun 29 09:47:50 2007 23, 23 -- Received: from cmtmail.cmt.anl.gov (CMTMAIL.cmt.anl.gov [146.139.148.212]) 23, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TElnrn006976 23, 23 -- for {microscopy-at-sparc5.microscopy.com} ; Fri, 29 Jun 2007 09:47:50 -0500 23, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 23, 23 -- Content-class: urn:content-classes:message 23, 23 -- MIME-Version: 1.0 23, 23 -- Content-Type: text/plain; 23, 23 -- charset="us-ascii" 23, 23 -- Subject: RE: [Microscopy] kV or keV? 23, 23 -- Date: Fri, 29 Jun 2007 09:47:48 -0500 23, 23 -- Message-ID: {661C1AA445464649B30D32151A7C34FA043C7DEE-at-CMTMAIL.cmt.anl.gov} 23, 23 -- In-Reply-To: {200706290927.l5T9RDE5025673-at-ns.microscopy.com} 23, 23 -- X-MS-Has-Attach: 23, 23 -- X-MS-TNEF-Correlator: 23, 23 -- Thread-Topic: [Microscopy] kV or keV? 23, 23 -- Thread-Index: Ace6L7L/rkLJ0aC5SA6Y1DpOvncfKwAKk7aA 23, 23 -- References: {200706290927.l5T9RDE5025673-at-ns.microscopy.com} 23, 23 -- From: "Fortner, Jeffrey A." {fortner-at-cmt.anl.gov} 23, 23 -- To: {nizets2-at-yahoo.com} , {microscopy-at-ns.microscopy.com} , 23, 23 -- {Microscopy-at-microscopy.com} 23, 23 -- Content-Transfer-Encoding: 8bit 23, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5TElnrn006976 ==============================End of - Headers==============================
When one of your students blows the turbo pump, you'll wish you had a diffusion pump. The diff-pump will last longer at a significant cost reduction. However, most are water cooled, and some facilities people don't like to see you dumping water continuously down the drain.
JQuinn
} From mail-at-ns.microscopy.com Thu Jun 28 20:29:42 2007 } Date: Thu, 28 Jun 2007 19:33:54 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: metzger-at-geosun.sjsu.edu } Reply-to: metzger-at-geosun.sjsu.edu } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] viaWWW: Electron microprobe - carbon coater } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both metzger-at-geosun.sjsu.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: metzger-at-geosun.sjsu.edu } Name: Ellen Metzger } } Organization: San Jose State University } } Title-Subject: [Filtered] Electron microprobe - carbon coater } } Question: We are in the process of setting up a probe lab and are in search of a carbon coater. I'd appreciate advice about carbon fiber versus rod and turbo pumped versus non-turbo for microprobe analysis of polished thin sections. } } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Thu Jun 28 19:33:12 2007 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5T0XA4N012579 } 8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 19:33:12 -0500 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240804c2aa03ba31cc-at-[206.69.208.22]} } 8, 11 -- Date: Thu, 28 Jun 2007 19:33:09 -0500 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: metzger-at-geosun.sjsu.edu (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: Electron microprobe - carbon coater } 8, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 12 -- From jquinn-at-www.matscieng.sunysb.edu Fri Jun 29 11:36:01 2007 8, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TGa01I000673 8, 12 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 11:36:01 -0500 8, 12 -- Received: (from jquinn-at-localhost) 8, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id l5TGVen28841 8, 12 -- for microscopy-at-microscopy.com; Fri, 29 Jun 2007 12:31:40 -0400 8, 12 -- Date: Fri, 29 Jun 2007 12:31:40 -0400 8, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 8, 12 -- Message-Id: {200706291631.l5TGVen28841-at-www.matscieng.sunysb.edu} 8, 12 -- To: microscopy-at-microscopy.com 8, 12 -- Subject: re: carbon coater ==============================End of - Headers==============================
I've got to take issue with this disparaging information about carbon string evaporation.
Carbon string gets criticized in the way given below, and I think that some of this comes from using it with rotary pumped vacuum levels, but possibly also the method of evaporation; the method usually given is "flash evaporation". We pump down to at least 2x10^-5 Torr (~ 0.0027 Pa) and evaporate in a series of pulses that bring the string to just white-hot and then switch off for a couple of seconds, repeating until the string "sparkles" the final remaining fibers. I have found the method highly repeatable and easy to arrive at a repeatable thickness. Call this "pulsed evaporation"?
We use 2 different systems , neither designed for carbon string. One is a Balzers BA080T turbo-pumped unit, and the other is a Kinney KDP-2A (circa 1965) ODP pumped system. For the Kinney, easier to explain, I use a short 1.25cm spacing tungsten boat holder (like the Fullam No. 12330: EFFA® Tungsten Boat Kit) and just lightly clamp the carbon string (SPI #11433 - 0.8mm dia) where the boat would normally go - 1.25 cm exposed length. This is set at a distance chosen for the desired thickness, ~5.5 -8cm typical. The system is pumped down to below 1x10^-4 Torr (2x10^-5 Torr typ.) and the string is degassed by slowly raising the voltage to give a medium bright orange glow - much faster vacuum recovery than with rods - and then switched OFF. The Variac (controls the input to the power transformer) is then set for ~40% (without power applied!) (this is 40% of max, max being 24VAC nominal of the power transformer secondary). The switch is flipped ON (view through dark glass, and as soon as it seems to have risen to white and stable temperature (~0.5s), switch off, repeating this cycle after a few seconds until burnout. This method was chosen to allow the carbon to reach evaporation temperature, but not send it all off at once. The radiation heating is minimized and spread over time. I coat the "back side" of Formvar that is placed over 5mm holes (for the method of picking up sections with a 1x2mm slot grid and setting the grid on the upper non-carbon-coated side of the filmed area) and I see almost no damaged films using this method, and had much trouble trying this with rods (1/8", turned down to 1mm dia x 2mm long tips).
We use a bit of very white paper stock stuck under the mica, etc., as an indicator of thickness by color; looking through my notebook, it is highly repeatable. In making adjustments to vary thickness, keep in mind that the thickness is inverslely proportional to the SQUARE of the distance; a small change in distance has a substantial effect on the film thickness.
By setting the voltage and pulsing time appropriately, it should be possible to get good coverage of rotating samples as well.
Different yarns can also be used, selecting a voltage to give similar characteristics to the evaporation. It seems that the SPI #11433 thread just comes to a stable white heat in ~0.5s, and that will vary for different product diameters. Most EM supply vendors carry a selection of thicknesses. The "thread" mentioned above gives a nice balance between thickness and heating. Thicker thread could be used at longer distances. One could experiment with different lengths as well; we have good success with the 1.25cm length. The paper cited below was very specific about the lengths used - he did NOT get good results with the length we use, but he did use "flash" evaporation. Unfortunately, technical data regarding the voltages and type of power supply are not given in that paper.
=================================================== Precise and reproducible deposition of thin and ultra-thin carbon films by flash evaporation of carbon yarn in high vacuum. Klaus-Ruediger Peters. J. Micros. (133), p 17. 1984.
His "gun" design was a covered chamber that included a yarn reservoir. The yarn was clamped between 2 posts 6mm separated, and the yarn was exposed through a hole. Details in paper... With the cover removed it gave ~50% thicker films (does not state if quality differences) Shorter lengths gave thicker films... {5 mm length emitted as a shower of sparks, gave substantial variations in thickness } 9 mm lengths also resulted in high variation in measured thickness. 6 mm gave the most reliable depositions. ======================================================
There are some things I don't fully understand in this paper - like that shorter lengths gave thicker films??? - maybe for shorer than 5mm lengths MOST fibers fully span the electrodes and it changes the characteristics of evaporation.
But if you have the apparatus to try this method, I strongly encourage trying it. It is economical and repeatable and the film quality is excellent.
Dale Callaham The University of Massachusetts -at- Amherst
edelmare-at-muohio.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Ellen: } } Turbo pumped systems pump faster. They pump to a lower pressure than } rotary pump only systems (i.e. table top models). Lower pressure } equals finer coating grain size. } } Fiber (rope, cord, etc.) is a quick and easy carbon coat, and can } get away with higher pressures, but it is rougher, and can leave } "chunks" of fiber on sample. Not particularly controlable as to } thickness (use more fibers vs fewer fibers). } } Rods systems tend to be finer coating, more controlable as to } "thickness" (evaporate more or less of the rod). } } If you are thinking about EBSD work in the future, and might wish to } use a very thin carbon coat (yes, before someone yells out, it will } obsure some of the signal). The control of the rod systems is } needed. } } I have not compared fiber vs rod for electron microprobe work on } polished sections. We use a full blown denton vacuum evaporator } (very fine, very versitile, very slow), and I really wish I could } get a turbo pumped table top model to easy speed for most SEM work. . } . } } } On 28 Jun 2007 at 19:33, metzger-at-geosun.sjsu.edu wrote: } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when replying } } please copy both metzger-at-geosun.sjsu.edu as well as the MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: metzger-at-geosun.sjsu.edu } } Name: Ellen Metzger } } } } Organization: San Jose State University } } } } Title-Subject: [Filtered] Electron microprobe - carbon coater } } } } Question: We are in the process of setting up a probe lab and are in } } search of a carbon coater. I'd appreciate advice about carbon fiber } } versus rod and turbo pumped versus non-turbo for microprobe analysis } } of polished thin sections. } } } } } } } } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } } 8, 11 -- From zaluzec-at-microscopy.com Thu Jun 28 19:33:12 2007 } } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5T0XA4N012579 } } 8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 19:33:12 -0500 } } 8, 11 -- Mime-Version: 1.0 } } 8, 11 -- Message-Id: {p06240804c2aa03ba31cc-at-[206.69.208.22]} } } 8, 11 -- Date: Thu, 28 Jun 2007 19:33:09 -0500 } } 8, 11 -- To: microscopy-at-microscopy.com } } 8, 11 -- From: metzger-at-geosun.sjsu.edu (by way of MicroscopyListserver) } } 8, 11 -- Subject: viaWWW: Electron microprobe - carbon coater } } 8, 11 -- Content-Type: text/plain; charset="us-ascii" } } ==============================End of - Headers============================== } } } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Director } 364 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } http://www.emf.muohio.edu } } "RAM disk is NOT an installation procedure." } } } ==============================Original Headers============================== } 13, 25 -- From edelmare-at-muohio.edu Fri Jun 29 09:33:34 2007 } 13, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) } 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TEXY0b026093 } 13, 25 -- for {microscopy-at-Microscopy.com} ; Fri, 29 Jun 2007 09:33:34 -0500 } 13, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) } 13, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l5TEXWYD015984; } 13, 25 -- Fri, 29 Jun 2007 10:33:32 -0400 } 13, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) } 13, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l5TEXWYU027030; } 13, 25 -- Fri, 29 Jun 2007 10:33:32 -0400 } 13, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} } 13, 25 -- To: metzger-at-geosun.sjsu.edu } 13, 25 -- Date: Fri, 29 Jun 2007 10:33:32 -0400 } 13, 25 -- MIME-Version: 1.0 } 13, 25 -- Subject: Re: [Microscopy] viaWWW: Electron microprobe - carbon coater } 13, 25 -- CC: microscopy-at-Microscopy.com } 13, 25 -- Message-ID: {4684DFFC.1900.E19503E-at-edelmare.muohio.edu} } 13, 25 -- Priority: normal } 13, 25 -- In-reply-to: {200706290033.l5T0Xu9c014602-at-ns.microscopy.com} } 13, 25 -- References: {200706290033.l5T0Xu9c014602-at-ns.microscopy.com} } 13, 25 -- X-mailer: Pegasus Mail for Windows (4.41) } 13, 25 -- Content-type: text/plain; charset=US-ASCII } 13, 25 -- Content-transfer-encoding: 7BIT } 13, 25 -- Content-description: Mail message body } 13, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 } ==============================End of - Headers==============================
==============================Original Headers============================== 14, 22 -- From dac-at-research.umass.edu Fri Jun 29 11:42:58 2007 14, 22 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37]) 14, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TGgwZt010117 14, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 11:42:58 -0500 14, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 14, 22 -- (authenticated bits=0) 14, 22 -- by race1.oit.umass.edu (8.13.7/8.13.7) with ESMTP id l5TGgvLn005589 14, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 14, 22 -- Fri, 29 Jun 2007 12:42:58 -0400 14, 22 -- Message-ID: {4685454D.5040909-at-research.umass.edu} 14, 22 -- Date: Fri, 29 Jun 2007 12:45:49 -0500 14, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 14, 22 -- Reply-To: dac-at-research.umass.edu 14, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.4) Gecko/20070509 SeaMonkey/1.1.2 14, 22 -- MIME-Version: 1.0 14, 22 -- To: edelmare-at-muohio.edu, Microscopy Listserver {Microscopy-at-microscopy.com} 14, 22 -- Subject: Re: [Microscopy] Re: viaWWW: Electron microprobe - carbon coater 14, 22 -- References: {200706291439.l5TEdpC1003194-at-ns.microscopy.com} 14, 22 -- In-Reply-To: {200706291439.l5TEdpC1003194-at-ns.microscopy.com} 14, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 22 -- Content-Transfer-Encoding: 8bit 14, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
You don't need to "dump water down the drain". Use a cooler/circulator to cool the diff pump.
Ted Dunn
----- Original Message ---- X-from: "jquinn-at-www.matscieng.sunysb.edu" www.matscieng.sunysb.edu} To: drteddunne-at-yahoo.com Sent: Friday, June 29, 2007 11:39:39 PM
Ellen and Folks
When one of your students blows the turbo pump, you'll wish you had a diffusion pump. The diff-pump will last longer at a significant cost reduction. However, most are water cooled, and some facilities people don't like to see you dumping water continuously down the drain.
JQuinn
} From mail-at-ns.microscopy.com Thu Jun 28 20:29:42 2007 } Date: Thu, 28 Jun 2007 19:33:54 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: metzger-at-geosun.sjsu.edu } Reply-to: metzger-at-geosun.sjsu.edu } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] viaWWW: Electron microprobe - carbon coater } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both metzger-at-geosun.sjsu.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: metzger-at-geosun.sjsu.edu } Name: Ellen Metzger } } Organization: San Jose State University } } Title-Subject: [Filtered] Electron microprobe - carbon coater } } Question: We are in the process of setting up a probe lab and are in search of a carbon coater. I'd appreciate advice about carbon fiber versus rod and turbo pumped versus non-turbo for microprobe analysis of polished thin sections. } } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Thu Jun 28 19:33:12 2007 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5T0XA4N012579 } 8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jun 2007 19:33:12 -0500 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240804c2aa03ba31cc-at-[206.69.208.22]} } 8, 11 -- Date: Thu, 28 Jun 2007 19:33:09 -0500 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: metzger-at-geosun.sjsu.edu (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: Electron microprobe - carbon coater } 8, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 12 -- From jquinn-at-www.matscieng.sunysb.edu Fri Jun 29 11:36:01 2007 8, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TGa01I000673 8, 12 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 11:36:01 -0500 8, 12 -- Received: (from jquinn-at-localhost) 8, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id l5TGVen28841 8, 12 -- for microscopy-at-microscopy.com; Fri, 29 Jun 2007 12:31:40 -0400 8, 12 -- Date: Fri, 29 Jun 2007 12:31:40 -0400 8, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 8, 12 -- Message-Id: {200706291631.l5TGVen28841-at-www.matscieng.sunysb.edu} 8, 12 -- To: microscopy-at-microscopy.com 8, 12 -- Subject: re: carbon coater ==============================End of - Headers==============================
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==============================Original Headers============================== 20, 20 -- From drteddunne-at-yahoo.com Fri Jun 29 12:15:46 2007 20, 20 -- Received: from web33407.mail.mud.yahoo.com (web33407.mail.mud.yahoo.com [68.142.206.139]) 20, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5THFjTx024600 20, 20 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 12:15:45 -0500 20, 20 -- Received: (qmail 20451 invoked by uid 60001); 29 Jun 2007 17:15:45 -0000 20, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 20, 20 -- s=s1024; d=yahoo.com; 20, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 20, 20 -- b=oAPM4ncnuS7eIslphcYIcl98y5a7De4cW3/ncJwuMeJkudBP/TwdhSoQX9tALupULJkdJoDOXG/k4iyeq6Yd5tD0wNpr6Bn97bPeCR1/WbyeehEeKaV/6Gqt8quxnJLid5lN14kvRMzirRjoW73Rp4mlEw8ckdqrCiUA+GquIkA=; 20, 20 -- X-YMail-OSG: KFaEDtwVM1kOzPL6LhGNUPWWLItwHWQodaz6XzfxVs74pczh4lX8w3iz_BfX_CgacEniJJ8H6CjMD.Bv.lie3yX0sHVIruNxDGbiL.MkXw_guPXRaY74GIg9QyIchyYHSVeaRNnEAwdY625HbvvFUESBMLMzxuMjzKBYKyno6ix3 20, 20 -- Received: from [125.24.166.46] by web33407.mail.mud.yahoo.com via HTTP; Fri, 29 Jun 2007 10:15:45 PDT 20, 20 -- X-Mailer: YahooMailRC/651.38 YahooMailWebService/0.7.41.16 20, 20 -- Date: Fri, 29 Jun 2007 10:15:45 -0700 (PDT) 20, 20 -- From: ted dunn {drteddunne-at-yahoo.com} 20, 20 -- Subject: Re: [Microscopy] re: carbon coater 20, 20 -- To: jquinn-at-www.matscieng.sunysb.edu 20, 20 -- Cc: microscopy-at-microscopy.com 20, 20 -- MIME-Version: 1.0 20, 20 -- Content-Type: text/plain; charset=ascii 20, 20 -- Message-ID: {412934.20093.qm-at-web33407.mail.mud.yahoo.com} ==============================End of - Headers==============================
Fwiw: It really shouldn't be all that hard with a bit of the right vacuum plumbing. I've been kluging stuff together for years out of a combination of economic necessity, and ultimately sheer pig-headedness and principle.
It strictly depends on whether 'doing it properly' or 'getting it done' is a higher priority. Your shop folks doubtless think you expect the former, since most people do.
If you go back and let them know that you are only concerned with the latter, and not cosmetics or perfection, they should be able to help you out nicely. The difference in thermal loss between a 'hard vacuum' and a 'soft vacuum' may mean you have to top it off a bit more frequently, but not by much. $500 will buy more than enough LN2 to cover the difference for years.
I don't want to tell your hardworking folks in the shop how to do their job, and every situation is unique anyway. If you can live with 'Plan B' run it past them again on a 'best efforts' basis.
If you strike out for one reason or another get back to me and I'll be happy to give you a hand.
Cheers,
b.
Rick Becker Cluster Sciences, L.L.C. Borolene Metamaterials 39 Topsfield Rd. Ipswich, MA 01938 US 978-337-9009 ionsourcerer-at-mac.com
If you don't know where you are going, call it "exploration". If you don't know what you are doing, call it "research".
==============================Original Headers============================== 11, 23 -- From ionsourcerer-at-mac.com Fri Jun 29 12:19:22 2007 11, 23 -- Received: from smtpout.mac.com (smtpout.mac.com [17.250.248.177]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5THJMax032364 11, 23 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 12:19:22 -0500 11, 23 -- Received: from mac.com (smtpin05-en2 [10.13.10.150]) 11, 23 -- by smtpout.mac.com (Xserve/smtpout07/MantshX 4.0) with ESMTP id l5THJLFL016827 11, 23 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 10:19:21 -0700 (PDT) 11, 23 -- Received: from [192.168.1.2] (pool-72-93-106-133.bstnma.fios.verizon.net [72.93.106.133]) 11, 23 -- (authenticated bits=0) 11, 23 -- by mac.com (Xserve/smtpin05/MantshX 4.0) with ESMTP id l5THJJJs006880 11, 23 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO); 11, 23 -- Fri, 29 Jun 2007 10:19:20 -0700 (PDT) 11, 23 -- Mime-Version: 1.0 (Apple Message framework v752.2) 11, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 11, 23 -- Message-Id: {5D66C9AB-F485-4C46-BD6D-5430C005FD77-at-mac.com} 11, 23 -- Content-Transfer-Encoding: 7bit 11, 23 -- From: Rick Becker {ionsourcerer-at-mac.com} 11, 23 -- Subject: Failing LN2 Dewars 11, 23 -- Date: Fri, 29 Jun 2007 13:19:17 -0400 11, 23 -- To: microscopy-at-microscopy.com 11, 23 -- X-Mailer: Apple Mail (2.752.2) 11, 23 -- X-Brightmail-Tracker: AAAAAA== 11, 23 -- X-Brightmail-scanned: yes ==============================End of - Headers==============================
I had been plagued with the dewar valve problem for several years before I found my own solution. Not even Duniway Stockroom will sell you an adaptor.
Cut the valve stem off and heliarc a KF fitting onto your new open port. The new fitting will allow you to outfit the opening with anything you can imagine. Vacuum gauges and valves of any style. My old 16 liter dewar will keep LN2 in it for three months if stored in a household refrigerator.
Early on I pumped the dewar to the low 10-3s. Now I just use a roughing pump. No difference in hold time.
I've done something similar with my early 1980s KEVEX EDS dewar. I've connected it to my system vacuum, but isolated with an ordinary vacumm valve. When it's dedicated thermocouple gauge climbs above 10 microns, I pump it down. Still has 159 ev at Mn. The original specification.
Bart Cannon Cannon Microprobe Seattle
==============================Original Headers============================== 6, 22 -- From cannonmp-at-comcast.net Fri Jun 29 13:10:49 2007 6, 22 -- Received: from sccrmhc12.comcast.net (sccrmhc12.comcast.net [63.240.77.82]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TIAmw0016537 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 13:10:49 -0500 6, 22 -- Received: from bartg84ti61htn (c-71-197-166-133.hsd1.or.comcast.net[71.197.166.133]) 6, 22 -- by comcast.net (sccrmhc12) with SMTP 6, 22 -- id {20070629181048012003odu7e} ; Fri, 29 Jun 2007 18:10:48 +0000 6, 22 -- Message-ID: {332d01c7ba78$b05faad0$85a6c547-at-bartg84ti61htn} 6, 22 -- From: "cannonmp" {cannonmp-at-comcast.net} 6, 22 -- To: {microscopy-at-microscopy.com} 6, 22 -- Subject: LN2 Dewar Valves 6, 22 -- Date: Fri, 29 Jun 2007 11:09:51 -0700 6, 22 -- MIME-Version: 1.0 6, 22 -- Content-Type: text/plain; 6, 22 -- format=flowed; 6, 22 -- charset="iso-8859-1"; 6, 22 -- reply-type=original 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-Priority: 3 6, 22 -- X-MSMail-Priority: Normal 6, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 6, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
Here is the July 2007 Microscopy Today table of contents. I will close the subscription list for this issue on Wednesday, July 6th, 2007.
Microscopists in North America and MSA members anywhere can subscribe for free. Anyone else may subscribe for US$50 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com Thank you.
Ron Anderson, Editor ================================ Probing Individual Proteins in Unsupported Membranes Stephen W. Carmichael, Mayo Clinic, Rochester, MN
Microbeam Analyses of the Most Challenging Extraterrestrial Samples Ever Returned Frans J.M. Rietmeijer, U. of New Mexico, Albuquerque, NM
Microscopy Today 2007 Salary Survey Results Ron Anderson and Barbara Foster,* Microscopy Today and *Microscopy/Microscopy &Education, Inc.
Fibrous Sepiolite Use As An Asbestos Substitute: Analytical Basics Louis Solebello, Int'l Asbestos Testing Lab. Inc., Mt. Laurel, NJ
Low Vacuum SEMs: Latest Generation Technologies and Applications William Neijssen, Ben Lich, & Pete Carleson* FEI Company, Eindhoven, The Netherlands and *Hillsboro, OR, USA
Luminance Contrast – a New Visible Light Technique for Examining Transparent Specimens Jörg Piper, Clinic Meduna, Bad Bertich, Germany
Temporal Evolution of Incipient Damage on Metallic Surfaces Analyzed by Unidirectional Laser Oblique Illumination (ULOI) E.A. Favret1,2, N.O. Fuentes2,3, & L. Ferrero2,1; Inst. de Tecnología Agropecuaria (INTA), 2Univ. Nacional de Gral San Martin, 3Comisión Nacional de Energía Atómica, Buenos Aires. Argentina
Multimode Trans-Illuminator for the Stereomicroscope Ted Clarke, Retired Materials Engineer
New Approaches to Managing, Marketing, and Money for Maintaining a Core Facility Developing a Financial Plan for the Long-term “Care and Feeding” of Major Equipment Alberto Rodriguez and Charlene Sullivan, Krannert School of Management, Purdue University
The College of Microscopy – Meeting Rapidly Growing Microscopy Demands Donald A. Brooks, The McCrone Group, Westmont, Illinois
Getting Epoxy Semi-Thin Sections to Stick to Glass Slides Gilbert (Gib) Ahlstrand, Univ. of Minnesota, St. Paul, MN
SEM Stub Holders for Sputter Coating at 90° Tilt Amanda Best, Central Michigan University, Mt. Pleasant, MI
Digital Cameras and the TEM Warren Straszheim, Iowa State University, Ames, Iowa
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both metzger-at-geosun.sjsu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: metzger-at-geosun.sjsu.edu Name: Ellen Metzger
Organization: San Jose State University
Title-Subject: [Filtered] Electron microprobe - carbon coater
Question: Thanks to all who responded to my previous posting - the information is most helpful.
I have one additional query. My colleagues in Engineering have a carbon coater for their SEM samples. Is their a difference in the requirements and thus the type of coater needed for SEM vs. electron microprobe (polished thin sections) samples?
==============================Original Headers============================== 8, 12 -- From zaluzec-at-microscopy.com Fri Jun 29 18:26:46 2007 8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5TNQhRQ015424 8, 12 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jun 2007 18:26:44 -0500 8, 12 -- Mime-Version: 1.0 8, 12 -- Message-Id: {p06240800c2ab45a2a5af-at-[206.69.208.22]} 8, 12 -- Date: Fri, 29 Jun 2007 18:26:41 -0500 8, 12 -- To: microscopy-at-microscopy.com 8, 12 -- From: metzger-at-geosun.sjsu.edu (by way of MicroscopyListserver) 8, 12 -- Subject: viaWWW: Thanks to all who responded to my previous posting - the 8, 12 -- information is most helpful. 8, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jeff-at-metallography.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jeff-at-metallography.com Name: Jeff Stewart
Organization: International Metallographic Society
Title-Subject: [Filtered] metallography contest deadline July 25
Question: A reminder that The International Metallographic Contest, co-sponsored by IMS and ASM International, is being held in conjunction with the M&M 2007 meeting in Ft. Lauderdale. The Contest has nine categories of competition which are primarily structured for materials analysts, and three categories for artistic photomicrographs. Best in Show will receive the Jacquet-Lucas Award and $3000.00. First Place winners in each category will receive $200.00. The Second Place winners will receive $100.00. Third Place winners will receive $50.00. A certificate of appreciation will be awarded for Honorable Mention. First Place undergraduate student winners in Classes 7 and 8 also receive the George L. Kehl Plaque.
It's not too late to enter. If you've already authored a poster presentation for the meeting, consider downsizing it to 16x20 and entering it in the IMC. If you've got a great looking photomicrograph, enter it in one of the artistic categories. Complete rules are available at www.internationalmetallographicsociety.org/contest.html. Mail entries to Bonnie Koske, HEICO Aerospace, 3000 Taft Street, Hollywood, FL 33021. Deadline is July 25.
Contact me off list if you have any questions.
Good luck to all entrants.
Jeff Stewart International Metallographic Contest Chair Metallographic Laboratory Manager Stern-Leach Company 49 Pearl Street Attleboro, MA 02703 Phone: 508-222-7400 x1329
No, the same units would be used for both applications with the same caveats as discussed. A probe sample _might_ require a bit more carbon than an SEM sample due to the probe currents involved, but the same coaters could be used.
Warren
-----Original Message----- X-from: metzger-at-geosun.sjsu.edu [mailto:metzger-at-geosun.sjsu.edu] Sent: Friday, June 29, 2007 6:28 PM To: wesaia-at-iastate.edu
Hi, everyone I am a M.S student and I would like to study the bacterial cytoskeletal elements for various bacteria (E. coli for the moment). I would like to label FtsZ, MreB or such proteins with lectins and then label them with gold or directly label these proteins with gold-labelled lectins and then observe the results in TEM.
Does anyone know any specific lectins for bacterial cytoskeletal proteins or anything about this subject?
Any help is appreciated Thank you in advance
Ceren Buyukkayalar M.S student Gebze Institute of Technology Turkey
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I once had a carbon coater with diff pump that I needed to cool independently from a TEM. I bought a domestic drinking water cooler and used a small submersible pump in a tank to circulate the water. It was quite cheap to put together and worked well.
Ted Dunn
----- Original Message ---- X-from: Jim Quinn www.matscieng.sunysb.edu} To: drteddunne-at-yahoo.com Sent: Saturday, June 30, 2007 12:43:55 AM
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