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From: modla-at-dbi.udel.edu
Date: Fri, 1 Jun 2007 07:50:14 -0500
Subject: [Microscopy] viaWWW: Metal Shadow Casting of DNA

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Email: modla-at-dbi.udel.edu
Name: Shannon Modla

Organization: Delaware Biotechnology Institute

Title-Subject: [Filtered] Metal Shadow Casting of DNA

Question: We are currently trying to rotary shadow cast DNA but are having problems obtaining consistent results with the metal shadowing. We are using an 80:20 platinum palladium wire purchased from EMS and a Denton Bench Top Turbo III sputter coater. The platinum palladium wire was wrapped around a 2-3 cm portion of a hairpin loop formed with tungsten wire. When the filament power is increased, the platinum palladium wire will either heat up without melting and evaporating or else the tungsten wire breaks without appreciable metal shadowing. I was wondering if anyone had any experience with this technique and could offer suggestions about how to obtain more consistent results.

Thanks,

Shannon Modla
Delware Biotechnology Institute
BioImaging Center


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From: xyang-at-SMU.CA
Date: Fri, 1 Jun 2007 08:32:08 -0500
Subject: [Microscopy] vendors on CL-microscope

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Hello all,

The geology department is looking for recommendation on getting a
Cathodoluminescence-Optical Microscope for geological research purpose.
Samples to be view would include but not limited to Zircon, Quartz, and
carbonates. We appreciate any recommendations and experiences you may have
on such instrumentation. Thanks in advance!

Best Regards,
----------------------------------------------
Xiang Yang
Electron Microscopy Center
Saint Mary's University
Science Building, Suite 135
Halifax, NS Canada B3H 3C3
Tel: (902) 496-8292 (lab)
(902) 420-5709 (off)
http://fgsr.smu.ca/emc/


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From: DusevichV-at-umkc.edu
Date: Fri, 1 Jun 2007 08:54:07 -0500
Subject: [Microscopy] RE: viaWWW: Question about image on Glass

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Allen,

Our UC-4 has never been re-evacuated. It has a yellow plastic cap over
the port, the port is filled with a pink grease, and under the pink
grease it looks like a metal ball. I can't say any more, but it looks
like the metal ball is original.

Diane Ciaburri

-----Original Message-----
X-from: oddioeng-at-aol.com [mailto:oddioeng-at-aol.com]
Sent: Thursday, May 31, 2007 10:53 PM
To: Ciaburri, Diane A.

What for did you "spin 200nm PMMA on the surface?"
As I understand, you have just glassy flat surface of resin on your
specimen, with no topography and nothing to observe.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy


}
} Email: yitianp-at-ece.arizona.edu
} Name: Yitian Peng
}
} Organization: University of Arizona
}
} Title-Subject: [Filtered] Question about image on Glass.
}
} Question: Hi everyone,
} I have some patterned Cr structure(width:100micro,
} height:60nm) on Glass.
} Then I spin 200nm PMMA on the surface.
} Then I Sputter 10nm Cr on the PMMA.
} I want to using JSM 6400 SEM to look at the structure.
} But I can't see anything?/
} What's the problem?/
} Thank you very much.
} Yitian
}
}
} --------------------------------------------------------------
} -------------
}
} ==============================Original
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From: kraftpiano-at-gmail.com
Date: Fri, 1 Jun 2007 09:06:48 -0500
Subject: [Microscopy] Unfortunate announcement.

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I'm not entirely sure if this is appropriate for this list, and if it
isn't I apologize in advance. I was just informed yesterday that my
position as science teacher at Atlantis Academy has been eliminated,
and that the math teacher will be teaching both subjects. It is
unfortunately a sad commentary on the direction that our world is
headed in.

I am making an appeal to anyone in the south Florida area who may need
some extra lab help, or anything that I can do. My prospects for
getting a new teaching job are not good until later in the summer, but
I would really like to start working somewhere locally as a lab
assistant so I can start working on my masters degree.

I know this is a bit of a shot in the dark, but I figured there'd be
no harm in asking.

Thank you all for your kind support over the last few months on our
SEM project. I am truly sorry that I will not be able to see it come
to fruition.

--Justin A. Kraft

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From: smalinskas-at-yahoo.com
Date: Fri, 1 Jun 2007 09:44:16 -0500
Subject: [Microscopy] RE: viaWWW: Question about image on Glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was once given the task of looking at a glassy
surface in the SEM. Just to rough in the focus was a
challenge. I sprinkled some powder on the surface to
provide a starting point for focus. Maybe this would
help you get started.

Otherwise, I agree with Vladimir... that PMMA - though
optically transparent - is not electron transparent,
and all you'll see is the Cr-sputter-coated polymer
surface.

Stu Smalinskas, P.E.
Metallurgist
SKF USA
Plymouth, Michigan
(734)414-6862

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Vladimir wrote:

What for did you "spin 200nm PMMA on the surface?"
As I understand, you have just glassy flat surface of
resin on your specimen, with no topography and nothing
to observe.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Yitian wrote:

Title-Subject: [Filtered] Question about image on
Glass.

Question:
Hi everyone, I have some patterned Cr structure
(width: 100micro, height: 60nm) on Glass. Then I spin
200nm PMMA on the surface. Then I Sputter 10nm Cr on
the PMMA. I want to using JSM 6400 SEM to look at the
structure. But I can't see anything? What's the
problem?

Thank you very much.

Yitian

Email: yitianp-at-ece.arizona.edu
Name: Yitian Peng
Organization: University of Arizona




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From: dac-at-research.umass.edu
Date: Fri, 1 Jun 2007 09:55:16 -0500
Subject: [Microscopy] Re: viaWWW: Metal Shadow Casting of DNA

Contents Retrieved from Microscopy Listserver Archives
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Shannon,

I don't know the particulars of operation of the evaporator unit and my
knowledge is for pure gold or platinum (some metals will alloy with the
tungsten and cause problems - maybe someone else knows).

Platinum MP = 1772 C
Tungsten MP = 3410 C

If the Pt wire is not melting, then the tungsten temperature is too low.
If you advance the filament heating slowly (a few seconds for work, but
slower in testing until you know the behaviour) you should first see the
Pt:Pd wire melt and flow to the bottom of the hairpin (the point of the
V is down) and the droplet will evaporate at a slightly higher
temperature. Actually you only need to put the wire near the tip of the V.

Just remember to heat the filament over a few seconds, not instantly.
Tungsten has a positive temperature coefficient and the filament
resistance is initially low and a large surge will occur if full voltage
is applied directly - it can cause the evaporant wire to spit or the
filament to burn out; but you shouldn't need to be that close to the
tungten melting point.

You don't specify distances but it sounds like you are using a lot of
wire. You probably only need 10-20Å. 0.5 cm of 8 mil wire at 10 cm
distance will give ~13 Å (at normal incidence, or the sides of DNA so
disposed....)

Å = (40.3 * diameter^2 * Length) / distance^2

diameter in "mils" (0.001"), 8 mils is a standard size, 0.008" dia
length of wire evaporant, distance to sample in cm

I have an Excel spreadsheet with a "calculator" for this formula that I
can send if you want - can't go to the Microscopy Listserver..... Just ask.

Hope this helps.

Dale Callaham
Univ. of Massachusetts, Amherst


modla-at-dbi.udel.edu wrote:
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} ---------------------------------------------------------------------------
}
} Email: modla-at-dbi.udel.edu
} Name: Shannon Modla
}
} Organization: Delaware Biotechnology Institute
}
} Title-Subject: [Filtered] Metal Shadow Casting of DNA
}
} Question: We are currently trying to rotary shadow cast DNA but are having problems
obtaining consistent results with the metal shadowing. We are using an
80:20
platinum-palladium wire purchased from EMS and a Denton Bench Top Turbo
III sputter coater.
The platinum palladium wire was wrapped around a 2-3 cm portion of a
hairpin loop formed
with tungsten wire. When the filament power is increased, the platinum
palladium wire will
either heat up without melting and evaporating or else the tungsten wire
breaks without
appreciable metal shadowing. I was wondering if anyone had any
experience with
this technique and could offer suggestions about how to obtain more
consistent results.
}
} Thanks,
}
} Shannon Modla
} Delware Biotechnology Institute
} BioImaging Center
}
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
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From: kenconverse-at-qualityimages.biz
Date: Fri, 1 Jun 2007 10:14:02 -0500
Subject: [Microscopy] test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sorry to occupy your mailbox, but I've had a couple of posts that didn't.
Ken


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Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
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kenconverse-at-qualityimages.biz
qualityimages.biz






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7, 27 -- From kenconverse-at-qualityimages.biz Fri Jun 1 10:14:02 2007
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From: kross-at-laurentian.ca
Date: Fri, 1 Jun 2007 10:15:10 -0500
Subject: [Microscopy] Oxford, EDS deadtime

Contents Retrieved from Microscopy Listserver Archives
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I have recently had a power outage and when I re-started my JEOL 6400 equipped OXFORD INCA X-sight the INCA software gave me an error stating "(1426)DSPP Failed Board" and my deadtime is 99% even with no beam. Can anyone help? Are the two errors related?
Thanks
Kirk


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From: kross-at-laurentian.ca
Date: Fri, 1 Jun 2007 11:01:03 -0500
Subject: [Microscopy] Oxford, EDS deadtime, problem solved

Contents Retrieved from Microscopy Listserver Archives
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when all else fails, reboot and your problems will be solved
thanks
Kirk


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From: gary-at-gaugler.com
Date: Fri, 1 Jun 2007 11:06:06 -0500
Subject: [Microscopy] Re: Oxford, EDS deadtime

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

They might be related. However, first thing to do is
to home the stage. There should be an option in the
SEM control to initialize the stage. Do this. If the
stage uses LEDs, this will drive the EDS nuts with high
cps and high DT. Restart PC.

If this does not fix the problem, then you might have
a damaged board from the power outage.

gary g.


At 07:16 AM 6/1/2007, you wrote:




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From: mdufraine-at-ebsciences.com
Date: Fri, 1 Jun 2007 11:21:42 -0500
Subject: [Microscopy] Re: viaWWW: Advice on critical point dryers

Contents Retrieved from Microscopy Listserver Archives
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Margret-

Energy Beam Sciences is the US distributor for Quorum Technologies,
which handles both the Polaron, and Emitech line of preparation
equipment, including Critical Point Dryers.

Quorum offers both a horizontal and vertical style CPD system, with the
horizontal style the user has the advantage of working with small or
large samples based on the chamber set-up, with the vertical system
offering more of the automatic features.

I'll look to contact you off-line to share details on the systems and
your needs.

Regards,

Mike Dufraine
Energy Beam Sciences,Inc.

mdienelt-at-msn.com wrote:
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} Email: mdienelt-at-msn.com
} Name: Margaret Dienelt
}
} Organization: USDA, ARS, National Arboretum
}
} Title-Subject: [Filtered] Advice on critical point dryers
}
} Question: Hello everyone,
}
} I've just learned we might have funds to replace our ancient,
} tempermental critical point dryer and would greatly appreciate any
} feedback anyone can give me on their CPD. I'm especially interested
} in reliability - the valves in our current unit have been a problem
} for years, requiring numerous repairs (usually after destroying a few
} samples).
}
} In addition to information anyone can share on specific models, I
} also have two general questions:
}
} What the pros and cons are to the two profiles, i.e. horizontal (like
} Tousimis) and vertical (like Polaron)?
}
} What are pros and cons to automatic vs manual models?
}
} Basically, any wisdom you'd like to share about purchasing a new
} critical point dryer will be more than welcome. Our new one will be
} used in a plant pathology lab and will be used to process primarily
} plant tissue. We don't need one for a clean room or one with the
} jumbo chamber.
}
} I'm sending this from my personal e-mail account since I'm having
} problems with my government e-mail, but if replies are sent to
} mdienelt-at-msn.com or margaret.dienelt-at-ars.usda.gov. I'll receive them
} one way or the other.
}
} Thank you!
}
} Margaret
}
}
}
} Margaret Dienelt
} Plant Pathologist/Electron Microscopist
} Floral and Nursery Plants Research Unit
} National Arboretum, ARS, USDA
}
} 10300 Baltimore Avenue
} Beltsville, MD 20705
}
} (301)504-6097
}
}
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} ==============================Original Headers==============================
} 20, 11 -- From zaluzec-at-microscopy.com Thu May 31 15:08:33 2007
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} ==============================End of - Headers==============================
}

--
Michael F. Dufraine
EM - Product Manager
TEL 800-992-9037 X340
MDufraine-at-ebsciences.com
www.ebsciences.com


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From: beaurega-at-westol.com
Date: Fri, 1 Jun 2007 12:00:14 -0500
Subject: [Microscopy] Re: test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ken,

No problem but you bring up a good point. I sent a posting and it didn't
show up on the list server. A recent one involved a person in IL. I sent
him a direct posting and a dseparate email posting to the list server.
Nothing bounced back from either email but the later posting never made it
to the list.

I fact, I see replies to postings before I ever see the original posting.
I know things get delayed and that delays happen. It's not Nestor's fault.
'It's a server time slicing or sharing thing', IMO.
In the next day or two, I might get the original posting. I have no
problem there. My problem is that sometimes I never get or will ever
receive the original posting (even my own).

So the other day after my posting failed, I sent a short posting to test my
ability to still post to the list. I relied to:
Re: [Microscopy] Re: AskAMicroscopist: Image Pro Analysis Question - URL
with an Image and micrscopy-at-microscopy.com.
My reply was posted and my posting received a reply from Mike Bode.

After a week and a half, my first posting still has never showed up.

I can see why some postings come through in double postings. I figure
members know this 'lack of posting' problem and so they send double postings.

Paul

At 10:14 AM 6/1/07 -0500, you wrote:
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From: info-at-nomadmet.com
Date: Fri, 1 Jun 2007 17:45:12 -0500
Subject: [Microscopy] viaWWW: looking for Image Analysis software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I apologize if I was supposed to post this response to the list. Sometimes
I don't see if a message comes from the group or an individual...

dj

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Email: info-at-nomadmet.com
Name: Tom Doggart

Organization: Nomad Metallurgy, Inc

Title-Subject: [Filtered] Image Analysis software

Question: I am looking for a user friendly image analysis package to use with my Spot and Nikon cameras and/or their JPEG files. I would like it to do the following at a minimum:



A) Various linear and area measurement
B) Phase % calculations for at least 3 phases in a structure
C) Image Montague or stitching
D) Expanded focus capabilities



Do you have any experience with any system that can do this?


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From: phillipst-at-missouri.edu
Date: Fri, 1 Jun 2007 17:50:22 -0500
Subject: [Microscopy] Re: viaWWW: looking for Image Analysis software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Regardless of which software package you go with, my recommendation for
your first step is to save your images as TIFFs!


At 05:47 PM 06/01/07, you wrote:



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Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu
http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/


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From: gary-at-gaugler.com
Date: Fri, 1 Jun 2007 17:55:26 -0500
Subject: [Microscopy] Re: viaWWW: looking for Image Analysis software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I use analySIS Opti s/w with Prior E103 Z control
to do slice stacking. I don't know if Opti will
control your cameras. I use Pixera 600CL and Olympus
DP-70 (same driver). Opti controls the Prior stage.

There are several stitching programs. They all have
plusses and minusses.

gary g.


At 02:47 PM 6/1/2007, you wrote:




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From: jquinn-at-www.matscieng.sunysb.edu
Date: Sat, 2 Jun 2007 11:51:44 -0500
Subject: [Microscopy] Re: viaWWW: looking for Image Analysis software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

ImageJ
http://rsb.info.nih.gov/ij/


--
Shawn Mikula, Ph.D.,
Postdoctoral Scholar
Center for Neuroscience
University of California-Davis,
1544 Newton Court,
Davis, CA 95618,
Phone: 530-754-9209
Fax: 530-754-9136
mail: samikula-at-ucdavis.edu
web: http://brainmaps.org



----- Original Message -----
X-from: {info-at-nomadmet.com}
To: {samikula-at-ucdavis.edu}
Sent: Friday, June 01, 2007 3:53 PM

I have used VIS by Visiopharm. It has incredible segmentation capabilities
that are attached to a database. You can build essentially a program to
separate an image into the regions of interest and then to all sorts of
calculations. This is all done by simple clicking.

----- Original Message -----
X-from: {info-at-nomadmet.com}
To: {rboehrin-at-vt.edu}
Sent: Friday, June 01, 2007 6:53 PM

Tom

Definitely do not use JPEG. Why spend the
money on a quality microscope and quality camera,
and then save the image in a bad file-format?

For "expanded (sic extended) focus", you should
consider using CombineZ5 (or ZM) and/or Helicon
Focus. Each has excellent support and cost.

I would also plug ImageJ and the excellent support/cost.

regards,

Jim


} From mail-at-ns.microscopy.com Fri Jun 1 18:44:41 2007
} Date: Fri, 1 Jun 2007 17:46:48 -0500
} To: jquinn-at-www.matscieng.sunysb.edu
} From: info-at-nomadmet.com
} Reply-to: info-at-nomadmet.com
} X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com}
} Subject: [Microscopy] viaWWW: looking for Image Analysis software
} Errors-To: MicroscopyListSpamFilter-at-microscopy.com
} X-lewp: MicroscopyListSpam NAGS
}
}
}
}
} ----------------------------------------------------------------------------
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} ----------------------------------------------------------------------------
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} ---------------------------------------------------------------------------
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} please copy both info-at-nomadmet.com as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: info-at-nomadmet.com
} Name: Tom Doggart
}
} Organization: Nomad Metallurgy, Inc
}
} Title-Subject: [Filtered] Image Analysis software
}
} Question: I am looking for a user friendly image analysis package to use with my Spot and Nikon cameras and/or their JPEG files. I would like it to do the following at a minimum:
}
}
}
} A) Various linear and area measurement
} B) Phase % calculations for at least 3 phases in a structure
} C) Image Montague or stitching
} D) Expanded focus capabilities
}
}
}
} Do you have any experience with any system that can do this?
}
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
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}


==============================Original Headers==============================
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9, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33])
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9, 12 -- Subject: Re: [Microscopy] viaWWW: looking for Image Analysis software
==============================End of - Headers==============================




From: bfoster-at-mme1.com
Date: Mon, 4 Jun 2007 00:17:14 -0500
Subject: [Microscopy] viaWWW: Metal Shadow Casting of DNA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sharon,

If the DNA is already attached to a substrate,
I'd really suggest that you try AFM instead. It
will give you essentially atomic level
resolution, without any disruptive coating.

Contact me off line if you need more info.

Thanks
Barbara

At 12:12 AM 6/4/2007, dac-at-research.umass.edu wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America




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From: cgarber-at-2spi.com
Date: Mon, 4 Jun 2007 00:59:12 -0500
Subject: [Microscopy] Shadowing with Pt for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dale Callaham wrote:
==================================================================
} I don't know the particulars of operation of the evaporator unit and my
} knowledge is for pure gold or platinum (some metals will alloy with the
} tungsten and cause problems - maybe someone else knows).
}
} Platinum MP = 1772 C
} Tungsten MP = 3410 C
}
} If the Pt wire is not melting, then the tungsten temperature is too low.
} If you advance the filament heating slowly (a few seconds for work, but
} slower in testing until you know the behaviour) you should first see the
} Pt:Pd wire melt and flow to the bottom of the hairpin (the point of the
} V is down) and the droplet will evaporate at a slightly higher
} temperature. Actually you only need to put the wire near the tip of the V.
}
} Just remember to heat the filament over a few seconds, not instantly.
} Tungsten has a positive temperature coefficient and the filament
} resistance is initially low and a large surge will occur if full voltage
} is applied directly - it can cause the evaporant wire to spit or the
} filament to burn out; but you shouldn't need to be that close to the
} tungten melting point.
}
} You don't specify distances but it sounds like you are using a lot of
} wire. You probably only need 10-20Å. 0.5 cm of 8 mil wire at 10 cm
} distance will give ~13 Å (at normal incidence, or the sides of DNA so
} disposed....)
}
} Å = (40.3 * diameter^2 * Length) / distance^2
}
} diameter in "mils" (0.001"), 8 mils is a standard size, 0.008" dia
} length of wire evaporant, distance to sample in cm
=================================================================
I have always thought that the best way to "shadow" was to use the "Pt/C sesile drop" method. I learned about it many
years ago in graduate school and don't know who used it originally. But one gets a finer grain if Pt/C is evaporated
simultaneously than if Pt alone is evaporated. The way to do this is to take a carbon rod that has been sharpened down
to a "neck", wrap about the "neck" not more than 30-40 mm of 8 mil Pt wire, put the bell jar onto the vacuum evaporator
but don't pump down. Slowly increase the current through the carbon rods to the point where, just beyond cherry red,
the Pt melts and because of surface tension effects, and the fact that liquid Pt does not want to wet carbon, it "beads
up", just as water does on a freshly waxed car. Once this happens, turn off the current, and when it cools down, rotate
the bead so that it is facing the surface to be shadowed.

Then the bell jar is put in place, the chamber pumped down, and the carbon rod is evaporated but what really comes off
is a combination of Pt and C.

Note: The optimum amount of wire to be used depends on a)distance to the substrate to be coated and b) shadowing angle.

Since Pt wants to alloy readily with W, this approach avoids all those other issues as well.

Disclaimer: SPI Supplies offers on our website Pt wire and presharpened carbon rods so we would have a vested interest
in seeing this technique used more rather than less frequently.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================

==============================Original Headers==============================
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From: donc-at-asmicro.com
Date: Mon, 4 Jun 2007 08:29:44 -0500
Subject: [Microscopy] Re: [a] viaWWW: Metal Shadow Casting of DNA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I second Barbara Foster's suggestion. For an example of DNA imaging by AFM,
see our website:
http://www.asmicro.com/Applications/DNA_Molecules.htm

regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]
=============================================
----- Original Message -----
From: bfoster-at-mme1.com
To: donc-at-asmicro.com
Sent: Monday, June 04, 2007 1:21 AM
Subject: [a] [Microscopy] viaWWW: Metal Shadow Casting of DNA





----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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Sharon,

If the DNA is already attached to a substrate,
I'd really suggest that you try AFM instead. It
will give you essentially atomic level
resolution, without any disruptive coating.

Contact me off line if you need more info.

Thanks
Barbara

At 12:12 AM 6/4/2007, dac-at-research.umass.edu wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Shannon,
}
} I don't know the particulars of operation of the evaporator unit and my
} knowledge is for pure gold or platinum (some metals will alloy with the
} tungsten and cause problems - maybe someone else knows).
}
} Platinum MP = 1772 C
} Tungsten MP = 3410 C
}
} If the Pt wire is not melting, then the tungsten temperature is too low.
} If you advance the filament heating slowly (a few seconds for work, but
} slower in testing until you know the behaviour) you should first see the
} Pt:Pd wire melt and flow to the bottom of the hairpin (the point of the
} V is down) and the droplet will evaporate at a slightly higher
} temperature. Actually you only need to put the wire near the tip of the
V.
}
} Just remember to heat the filament over a few seconds, not instantly.
} Tungsten has a positive temperature coefficient and the filament
} resistance is initially low and a large surge will occur if full voltage
} is applied directly - it can cause the evaporant wire to spit or the
} filament to burn out; but you shouldn't need to be that close to the
} tungten melting point.
}
} You don't specify distances but it sounds like you are using a lot of
} wire. You probably only need 10-20Å. 0.5 cm of 8 mil wire at 10 cm
} distance will give ~13 Å (at normal incidence, or the sides of DNA so
} disposed....)
}
} Å = (40.3 * diameter^2 * Length) / distance^2
}
} diameter in "mils" (0.001"), 8 mils is a standard size, 0.008" dia
} length of wire evaporant, distance to sample in cm
}
} I have an Excel spreadsheet with a "calculator" for this formula that I
} can send if you want - can't go to the Microscopy Listserver..... Just
ask.
}
} Hope this helps.
}
} Dale Callaham
} Univ. of Massachusetts, Amherst
}
}
} modla-at-dbi.udel.edu wrote:
} }
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
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} ----------------------------------------------------------------------------
} }
} } This Question/Comment was submitted to the Microscopy Listserver
} } using the WWW based Form at http://ns.microscopy.com/MLFormMail.html
}
} ---------------------------------------------------------------------------
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} a Subscriber, so when replying
} } please copy both modla-at-dbi.udel.edu as well
} as the MIcroscopy Listserver
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} ---------------------------------------------------------------------------
} }
} } Email: modla-at-dbi.udel.edu
} } Name: Shannon Modla
} }
} } Organization: Delaware Biotechnology Institute
} }
} } Title-Subject: [Filtered] Metal Shadow Casting of DNA
} }
} } Question: We are currently trying to rotary
} shadow cast DNA but are having problems
} obtaining consistent results with the metal shadowing. We are using an
} 80:20
} platinum-palladium wire purchased from EMS and a Denton Bench Top Turbo
} III sputter coater.
} The platinum palladium wire was wrapped around a 2-3 cm portion of a
} hairpin loop formed
} with tungsten wire. When the filament power is increased, the platinum
} palladium wire will
} either heat up without melting and evaporating or else the tungsten wire
} breaks without
} appreciable metal shadowing. I was wondering if anyone had any
} experience with
} this technique and could offer suggestions about how to obtain more
} consistent results.
} }
} } Thanks,
} }
} } Shannon Modla
} } Delware Biotechnology Institute
} } BioImaging Center
} }
} }
}
} ---------------------------------------------------------------------------
} }
} } ==============================Original
} Headers==============================
...
} } 9, 11 -- Date: Fri, 1 Jun 2007 07:50:12 -0500
} } 9, 11 -- To: microscopy-at-microscopy.com
} } 9, 11 -- From: modla-at-dbi.udel.edu (by way of MicroscopyListserver)
} } 9, 11 -- Subject: viaWWW: Metal Shadow Casting of DNA
} } 9, 11 -- Content-Type: text/plain; charset="us-ascii"
} } ==============================End of -
} Headers==============================
...
} 14, 21 -- From dac-at-research.umass.edu Fri Jun 1 09:55:15 2007
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} (race4.oit.umass.edu [128.119.101.40])
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} Fri, 1 Jun 2007 09:55:15 -0500



==============================Original Headers==============================
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From: kleopullin-at-pacbell.net
Date: Mon, 4 Jun 2007 14:48:57 -0500
Subject: [Microscopy] LM Optical physics text -- recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm reading Falk, Brill and Stork's "Seeing the Light:
Optics in Nature, Photography, Color, Vision, and
Holography." I like the book, in fact love it, but I
want something more dedicated to the physics/math. I
did order the Schaum's outline for problems.

Can someone recommend a good undergraduate level
optics text? My college level math and physics are
fine.

Has anyone used Eugene Hecht's Optics either as a
student or instructor for a course? Is it good enough
to actually buy, or are there better texts?

Thanks,

K. Leo Pullin

==============================Original Headers==============================
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From: gary-at-gaugler.com
Date: Mon, 4 Jun 2007 15:25:11 -0500
Subject: [Microscopy] viaWWW: looking for Image Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Please feel free to chastise me if I am wrong.

ImageJ is free and you get what you paid for.
I do not know whether it will control the Prior
stage controllers. If it does, great. Problem
solved.

However, if not, then you need industrial strength
software like analySIS--or current generation.
This controls the Z axis stepper and automatically
collects Z stacks and then assembles them into a
high depth of focus final image. I typically do
20 slices. OK....do this yourself manually and
try to get a good result. I did and after beating
my head against the wall too many times, it was
obvious that another method was necessary.

The focus assembly method is based on contrast.
So many small increments of Z are ideal. analySIS
and the Prior stage controller are seamlessly integrated.

gary g.




At 03:04 PM 6/1/2007, you wrote:
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From: mmiralles-at-pi.ac.ae
Date: Tue, 5 Jun 2007 00:06:07 -0500
Subject: [Microscopy] Sputter & Carbon Coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone,

Is there anybody here who has used the SPI Combined Sputter and Carbon Coater System? Any feedback? For those who have combined systems, have you encountered any contamination issues? How can this best be avoided?

Aside from good sputtering/coating capability, we are also considering having a dual system with smaller footprint since we have a very crowded lab. Any brand/model you can suggest?

Thank you so much.

Melina Miralles
Lab Technician
The Petroleum Institute
Abu Dhabi, UAE
 


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From: mike.reedy-at-cellbio.duke.edu
Date: Tue, 5 Jun 2007 11:24:22 -0500
Subject: [Microscopy] Re: lead citrate and block staining - correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Diana and others who were interested,

Dr Peters gives me permission today by email to quote on the
LisServer her clarification/correction, sent to me earlier today,
that only UrAc, but not Pb Citrate, was used as a block stain in her
work. Personally, I am quite grateful we were briefly misled,
because it brought in David Elliott's response on his use of lead
acetate block stain (in EtOH-acetone 50:50) following aqueous UrAc.

Here is Dr Peter's clarification/correction:
"Our method is as follows: We use uranyl acetate in 70% ethanol as a
block stain ....... then [after] the tissue is embedded in Epon and
polymerized..... We use the lead citrate only for the ready prepared
sections. I am sorry if my phrasing [turned out to] be kind of
misleading."

I plan continued exploration of both lead salts. Use as a block
stain in organic solvent after UrAc especially interests me for
freeze-substitution. So far, I find both are insoluble or nearly so
in 100% acetone, so the EtOH-containing vehicle in Elliott's
procedure seems necessary. Does anyone know or remember if the
extreme alkalinity of aqueous lead citrate section stains is
essential for releasing the Pb from the strongly sequestering
citrate? I'm not sure that pH 11 can be approached in pure organic
solvent, but why not try....?

I would guess that prolonging the exposure of tissue blocks to
organic solvent for extended block-staining is NOT likely to cause
additional shrinkage or extraction of cells, because I think the
binding of metals tends to stabilizes the components. ---not
completely against shrinkage however; I find that freeze-substitution
in acetone-TA followed by acetone UrAc fails to prevent variable
amounts of shrinkage, 5-12%, of the myofilament lattice spacing in
striated muscle; I'd like someday to see the process monitored by
x-ray cryo-diffraction to identify the stage where this occurs and
seek ways to prevent or minimize the shrinkage.

-mike reedy-

***************************
At 10:07 PM -0500 5/27/07, dianavd-at-eye.usyd.edu.au wrote:
----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Geoff McA asked for the reference to the paper I mentioned. It's
Peters S et al; American Journal of Ophthalmology (2007)
143:995-1002; Ultrastructural findings in the primate eye after
intravitreal injection of Bevacizumab. All it says is

"postfixed with 1% OsO4 at room temperature
in 0.1 M cacodylate buffer (pH 7.4) for three hours,
bloc-stained with uranyl acetate and lead citrate, and embedded
in Epon after dehydration in a graded series of acetones"

I've Emailed asking for more details. I have the PDF if anyone would
like to see the pics.


Diana

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} I've just read a paper where the author talks about block staining
} with uranyl acetate and lead citrate. UAc yes, but Pbcitrate? Has
} anyone heard of this? Does it work? The pictures in the paper were
} very nice; good contrast and detail. With the recent talk about
} general lack of contrast in specimens these days (I quite agree on
} that; I find contrast poorer now than in the past), could this be
} a new method?
}
} All opinions please!
}
} Cheers,
}
} Diana
}

--
-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
Dept. Cell Biology, Box 3011 (for U.S. Mail)
458 Alex Sands Bldg, Research Dr (courier)
Durham, NC 27710

Office 919-668-2534
Lab 919-684-5674
Fax 919-681-9929
mike.reedy-at-cellbio.duke.edu
http://note.cellbio.duke.edu/Faculty/Research/Reedy.html

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From: dianavd-at-eye.usyd.edu.au
Date: Tue, 5 Jun 2007 19:23:45 -0500
Subject: [Microscopy] Pb citrate block staining - followup

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

For those of you following the use of lead citrate as a block stain
discussion, I had a reply from the author of the paper where the
method was described. It turns out to have been incorrect; PbC was
not used as a block stain. It was an English usage problem - the
author is German - she actually used the PbC in the usual way as a
section stain. Still, the idea got us here on the List thinking!

Cheers,

Diana

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From: pcduncan-at-cyllene.uwa.edu.au
Date: Tue, 5 Jun 2007 20:41:03 -0500
Subject: [Microscopy] SEM - Electroscan E3 problem - require service engineer manual for initial setup procedure.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi - We have an old Electroscan which has proved to be fairly reliable
over the years, but lately it has been having problems. Recently, the
CPU board died and we were fortunate enough to have a spare (we
basically have a spare for everything with this machine). This spare
worked in that I could communicate with the CPU via its RS232 port and
have full access to its command set. With the self test the CPU board
says its OK. Unfortunately, we now seem to have no image coming
through. I've played with the command set menu hoping to get an image.
At one point I was able to see an extremely weak signal but I'm not
sure how I really achieved it simply because there are so many
different command options. What I want is Volume 6 - APPENDICES, from
the ElectroScan manual set (we have the others). This manual goes into
depth about the system software structure. What I also want, if
possible, is the service-engineer manual which would detail the actual
initial setup of the E3, and any advice in regard to my E3 problem is
really welcome.

Thanks

Peter Duncan
Senior Technician


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From: donovan.leonard-at-gmail.com
Date: Tue, 5 Jun 2007 22:13:59 -0500
Subject: [Microscopy] Table Top SEM & High School Nano Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Listsers -

Some days ago there was a question posed about the educational benefits
of table top SEM and nano-scale samples. In a few weeks I'll post my
experiences using a Hitachi TM-1000 table top SEM in a Nanotechnology
course for high schoolers as part of the Duke University Talent
Identification Program (TIP). More about the program can be found at
'www.tip.duke.edu'. I've chosen the TM-1000 to introduce the students
to the micro and nanoscale, and we will be using the SEM, in addition to
AFM and HRTEM to characterize Au and Ag nanoparticles the students
synthesize as part of a experiential learning activity.

In my opinion the table top SEM will allow the students hands-on
experience with an electron microscope. Not that it will resolve atomic
columns in nanoparticles, but as a way to introduce them to the scale of
things. The intention is to let the students choose ANY samples they
are interested in and let them discover the detail of features on the
micro and nanoscale.

If anyone has had experience integrating these microscopies into a high
school course on nanotechnology I would be most interested in learning
more about how it was accomplished and the outcomes. I would also be
more than happy to share the syllabus created for the course if requested.

Stay tuned, I'll post the student's data and feedback on the table top
SEM, AFM and TEM.

Donovan








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From: dljones-at-bestweb.net
Date: Wed, 6 Jun 2007 08:07:59 -0500
Subject: [Microscopy] Re: Table Top SEM & High School Nano Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Donovan,

I have helped get a SEM put into my local high school. The science teacher
there now is able to offer 8 college credits to students that take his
advanced program related to this instrument. Mark would be able to talk to
you more about that aspect and how he integrates it into his curriculum. I
have cc'd him with this email so you'll have his email address. This is
still in the beginning stages so there is a lot yet to know and learn. But
I think it is an absolutely fabulous opportunity for learning...

In the process of getting the SEM into my local high school, I have
learned a fair amount about SEM usage in high schools in the US and a bit
internationally. There are pockets of high schools around the USA that
have this kind of technology operating in high schools, but not a lot.
Germany has some high schools using it, apparently Japan has it
extensively used throughout their high school system.

You are using Au and Ag particles. My experience indicates high school
students seem more interested in biological samples rather than materials.
It would be very useful if a materials curriculum could be developed that
would interest students at this level.

Finding teachers and school systems that are open to this and have the
science background to make it work well, I think is a large hurdle. If a
solid curriculm could be put together that would work as a framework for
teachers to use, I think that would be very helpful.

Schools are, justifiably, reluctant to use this technology as maintaining
these machines is quite expensive and most schools run on very tight
budgets. In the cases where I have seen SEM's working in high schools,
there has usually been someone that is knowledgable about the instruments
and can maintain them for the school pro-bono. These individuals have been
key in getting SEM's into high schools. (There may be a program in western
PA that's an exception to this, but I've not had any luck contacting
them.)

Do send me your curriculum and further information about what you are
doing. I can give you more details of programs I've discovered if you
wish, but I don't think those details are of general interest to the list
(do correct me if I'm wrong).

dj

On Tue, 5 Jun 2007, donovan.leonard-at-gmail.com wrote:

} Hello Listsers -
}
} Some days ago there was a question posed about the educational benefits
} of table top SEM and nano-scale samples. In a few weeks I'll post my
} experiences using a Hitachi TM-1000 table top SEM in a Nanotechnology
} course for high schoolers as part of the Duke University Talent
} Identification Program (TIP). More about the program can be found at
} 'www.tip.duke.edu'. I've chosen the TM-1000 to introduce the students
} to the micro and nanoscale, and we will be using the SEM, in addition to
} AFM and HRTEM to characterize Au and Ag nanoparticles the students
} synthesize as part of a experiential learning activity.
}
} In my opinion the table top SEM will allow the students hands-on
} experience with an electron microscope. Not that it will resolve atomic
} columns in nanoparticles, but as a way to introduce them to the scale of
} things. The intention is to let the students choose ANY samples they
} are interested in and let them discover the detail of features on the
} micro and nanoscale.
}
} If anyone has had experience integrating these microscopies into a high
} school course on nanotechnology I would be most interested in learning
} more about how it was accomplished and the outcomes. I would also be
} more than happy to share the syllabus created for the course if requested.
}
} Stay tuned, I'll post the student's data and feedback on the table top
} SEM, AFM and TEM.
}
} Donovan


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From: Robert.Zonis-at-sanford.com
Date: Wed, 6 Jun 2007 10:48:53 -0500
Subject: [Microscopy] New microscope purchase advice

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Fellow Listers,

We unexpectedly have about $150,000 - $180,000 to potentially spend on a new microscope/digital vision system, and we are trying to determine which type of system would give us more relevant information about our product's components. We want to examine the following materials: the tips of ball point, fountain and roller ball pens (iron, tungsten carbide, brass, nickel-coated brass, nickel silver alloys, ceramic), plastic tip holders and ink reservoirs (polyethylene/polypropylene, nylon, PET), absorbent materials of various sizes and porosities (cellulosic, natural or artificial fibers). We are also interested in examining high-concentration micronized pigment dispersions to determine particle size (approximately 1 micron), shape and/or particle size distribution.

We are trying to decide between a tabletop SEM (either the Hitachi TM-1000 or the FEI Phenom) or a scanning laser microscope (Keyence VK-9700). We have a very limited amount of space to work with - anything larger than a desktop unit would require part of the budget to be spent on building a new room - and that is virtually impossible to get approved. Practically speaking, it's a desktop unit or nothing.

So, my questions are: Is there any way, short of sending many samples off to be examined, and then evaluating the results, to eliminate one or more of these units? Is there one that stands out for versatility or ease of use? How big a task is sample prep for these units? Additionally, have we missed a system to consider?

Please note that we have never looked at any of these components at higher than optical magnification before, so I can't tell you what features I need to see, because I have no idea what, if anything, that there is to see, or even whether or not the nanoscale detail makes a difference in the performance of our products. We will not be able to dedicate a person to the operation and maintenance of the system, so anything that takes much more than an hour or so of maintenance a week is going to be a big problem. I also need to point out that the closer the price gets to $100,000, the more competitive it is among all the instrumentation options we're considering, and the more likely it is to get approved.

Robert Zonis
Product Development Chemist, LMTC
Sanford L.P. - A Newell Rubbermaid Company
3 Sharpie Way
Shelbyville, TN 37160
robert.zonis-at-sanford.com
www.papermate.com


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From: bfoster-at-mme1.com
Date: Wed, 6 Jun 2007 12:23:44 -0500
Subject: [Microscopy] Re: New microscope purchase advice

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Robert,

I would encourage you to investigate NT-MDT's Tomo, a unique
combination of AFM and ultramicrotome, fitted with Media Cybernetics
3D constructor. It will provide the 3D information you need for
accurate particle sizing/shape characterization as well as
distribution calculations. Depending on how they are mounted, you
can also use the AFM to characterize the surfaces you described,
without having to coat or pump down a vacuum. Their new dealer in
the US is Abeam, in Castro Valley, CA.

Also, depending on the size of the surface features, a good reflected
light optical microscope, preferably fitted DIC or Hoffman Modulation
Contrast, and Polarizers, would go a long way toward routine
evaluations. MME provides specialized courses in both these
areas. If you are interested, please contact me off-line.

CAVEAT: MME no longer has any financial relationship with NT-MDT but
does make its living through customized, on-site courses.

Hope this was helpful,
Barbara Foster, President

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com


MME is now scheduling customized, on-site courses through
September. Call us today for details.

P. S.
Need a good general reference or light microscopy text for next
semester? Call us today to learn more about "Optimizing LIght
Microscopy". Copies still available through MME... even for
class-room lots ... and we give quantity discounts. Call Ken Piel at
(972)954-8011.



At 10:57 AM 6/6/2007, Robert.Zonis-at-sanford.com wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


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From: TindallR-at-missouri.edu
Date: Wed, 6 Jun 2007 15:14:44 -0500
Subject: [Microscopy] Carbon nanos in tissue: SEM/TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Every once in a while we get a request to image particles which have
been ingested by organisms, but this latest one seems especially tricky.

We are being asked to image carbon nanomaterials, such as nanowires, and
how they distribute themselves in the tissues and organs of living
organisms. My first search of the literature (ongoing, by the way)
indicates that I'm not the only one having trouble with this. Aside
from the obvious problems of differentiating 3-D structures in
nanometers-thick sections, there is the problem of seeing low-contrast
carbon in carbon-rich tissues.

My first thoughts were that adding electron density to the carbon
nanothingys would make them easier to see, both in thin sections and in
fractured specimens for SEM. At least this could help localize them,
even if seeing their true shapes remains a problem. How to do this is
another thing. Probably the particles would have to be augmented with
metals before being released into the organisms' environment, and that
would be a task for the makers and users of the nanoparticles. And
would that affect their final distribution in the creatures? From our
end, I'm trying to think of how to adapt immunolabeling techniques to
this.

Has anybody run across this problem and have thoughts they'd be willing
to share? The PI on this one has high hopes that we can put the
nano-rabbit of this tiny, little hat.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


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From: bfoster-at-mme1.com
Date: Wed, 6 Jun 2007 15:34:13 -0500
Subject: [Microscopy] Re: Carbon nanos in tissue: SEM/TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

This is another one of those "TOMO" applications.

As mentioned earlier, I have a movie made by Dr.deWith of the Dutch Polymer Institute (TU/E) showing the distribution of carbon nanotubes in epoxy. Because the AFM uses local differences in elasticity, there is none of the CNT in C-based tissue problem described below. Again, although I am no longer involved in this project, I am happy to forward a copy of the film to anyone who is interested. (It will come via www.YouSendIt.com, because of the large file size).

Dr. Anton Efimov of NT-MDT is the developer of this technique and is usually willing to run samples, schedule permitting. See his email in the CC: above.

NTA is no longer NT-MDT's agent here in the US. If you are interested in following this further, I recommend that you contact Dr. Yuri Bobrov, who is now with ABeam (Dealer principle, Dr. Sergey Babin - see email above).

As always, if there are further questions, please don't hesitate to call/email.

(Note: MME is no longer involved with this vendor).

Hope this was helpful,
Barbara Foster, President

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com


MME is now scheduling customized, on-site courses through September. Call us today for details.

P. S.
Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.




At 03:27 PM 6/6/2007, TindallR-at-missouri.edu wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


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From: schooley-at-mcn.org
Date: Wed, 6 Jun 2007 16:16:28 -0500
Subject: [Microscopy] Re: Table Top SEM & High School Nano Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm VERY interested. We need a database of dedicated people who are
doing high school SEM, so that experience, problems, curricula, etc.
can be shared. Will either of you be at M&M in Ft. Lauderdale?
Please come to the MICRO booth so that we can try to organize
something. Or send me an Email. If anyone else who is doing HS SEM,
including those associated with the FEI & RJ Lee school SEM programs,
is reading this, we need to talk to you too!

What is my motive, other than trying to get you together? Project
MICRO is MSA's outreach program for middle schools (see URL below).
I'd like to convince all of you to encourage introductory LM in your
"feeder" middle schools; SEM is an abrupt way to begin.

Caroline

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

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From: colijn.1-at-osu.edu
Date: Wed, 6 Jun 2007 16:18:27 -0500
Subject: [Microscopy] M&M 2007 detailed program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

Has the M&M 2007 daily schedule of papers and symposia been published
yet? I didn't find it on the meeting web site?

thanks,
Henk


Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all
at once. Lately it doesn't seem to be working.


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From: jenniferwal-at-cs.com
Date: Wed, 6 Jun 2007 19:16:34 -0500
Subject: [Microscopy] AskAMicroscopist: High school student looking for internship in

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jenniferwal-at-cs.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, June 6, 2007 at 13:16:12
---------------------------------------------------------------------------

Email: jenniferwal-at-cs.com
Name: Jennifer Wallace

Organization: Academic Coaching

Education: 9-12th Grade High School

Location: Evanston, IL USA

Question: I am working with a junior in high school who is very interested in this field. We are trying to find an internship or volunteer experience for her and need help! She is in the most rigorous science program at her high school and is an excellent photographer, which is why she is interested in this field in college and beyond.

---------------------------------------------------------------------------

==============================Original Headers==============================
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7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: jenniferwal-at-cs.com (by way of Ask-A-Microscopist)
7, 12 -- Subject: AskAMicroscopist: High school student looking for internship in
7, 12 -- Illinois
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==============================End of - Headers==============================




From: dac-at-research.umass.edu
Date: Thu, 7 Jun 2007 10:40:53 -0500
Subject: [Microscopy] Carbon nanos in tissue: SEM/TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Microscopists,

I know that vendors/vendor employees are active and involved in the MSA
and contribute greatly to the dialogs here. However, I personally feel
that this message reply is a bit over the top in commercializing the
List. Every question to the List can't be answered "you should try AFM"
and go on to plug the company and it's products. The response seems only
slightly related to the essence of the original question. How is AFM
going to visualize carbon nanotubes inside a cell that is inside a
tissue? This was a repeat of the response to recent technical questions
on vacuum metal shadowing technical problems. It is fine to bring
attention to alternate methods, but primarily we should be good
listeners and try to help people with the problem they have asked about.
Metal shadowing is a proven technique - with limitations, same as ALL
techniques - that people use routinely; one can scan relatively large
areas at high resolution for the best area. It is a method suitable to
the purpose. The DNA is already bulked up artificially by the
preparation method and the metal shadowing adds a known factor to that
size. But {visualizing} the DNA/plasmid is often the key, not the exact
molecular size. One vendor sent a link to an image of a 1um square scan
of DNA; the image was quite blurry, 2 nm/pixel resolution. I'm sure this
is a "display" result - the best they have. Finding a plasmid on a sheet
of carbon/mica for AFM scanning can't be as routine as the "traditional"
method of viewing the shadowed material in a TEM. It is a bit of
comparing apples and oranges.

So AFM is not the one answer to all questions, and please go light on
the commercial plugs. It seems that vendors were more respectful of this
"line" in the past.

Thanks for allowing my rant,

Dale Callaham



bfoster-at-mme1.com wrote:
} ----------------------------------------------------------------------------
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}
} Randy,
}
} This is another one of those "TOMO" applications.
}
} As mentioned earlier, I have a movie made by Dr.deWith of the Dutch Polymer Institute (TU/E) showing the distribution of carbon nanotubes in epoxy. Because the AFM uses local differences in elasticity, there is none of the CNT in C-based tissue problem described below. Again, although I am no longer involved in this project, I am happy to forward a copy of the film to anyone who is interested. (It will come via www.YouSendIt.com, because of the large file size).
}
} Dr. Anton Efimov of NT-MDT is the developer of this technique and is usually willing to run samples, schedule permitting. See his email in the CC: above.
}
} NTA is no longer NT-MDT's agent here in the US. If you are interested in following this further, I recommend that you contact Dr. Yuri Bobrov, who is now with ABeam (Dealer principle, Dr. Sergey Babin - see email above).
}
} As always, if there are further questions, please don't hesitate to call/email.
}
} (Note: MME is no longer involved with this vendor).
}
} Hope this was helpful,
} Barbara Foster, President
}
} Microscopy/Microscopy Education
} 313 S Jupiter Rd, Suite 100
} Allen, TX 75002
} P: 972-954-8011
} W: www.MicroscopyEducation.com
}
}
} MME is now scheduling customized, on-site courses through September. Call us today for details.
}
} P. S.
} Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
}
}
}
}
} At 03:27 PM 6/6/2007, TindallR-at-missouri.edu wrote:
}
}
}
} } ----------------------------------------------------------------------------
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} }
} } Dear Listers,
} }
} } Every once in a while we get a request to image particles which have
} } been ingested by organisms, but this latest one seems especially tricky.
} }
} } We are being asked to image carbon nanomaterials, such as nanowires, and
} } how they distribute themselves in the tissues and organs of living
} } organisms. My first search of the literature (ongoing, by the way)
} } indicates that I'm not the only one having trouble with this. Aside
} } from the obvious problems of differentiating 3-D structures in
} } nanometers-thick sections, there is the problem of seeing low-contrast
} } carbon in carbon-rich tissues.
} }
} } My first thoughts were that adding electron density to the carbon
} } nanothingys would make them easier to see, both in thin sections and in
} } fractured specimens for SEM. At least this could help localize them,
} } even if seeing their true shapes remains a problem. How to do this is
} } another thing. Probably the particles would have to be augmented with
} } metals before being released into the organisms' environment, and that
} } would be a task for the makers and users of the nanoparticles. And
} } would that affect their final distribution in the creatures? From our
} } end, I'm trying to think of how to adapt immunolabeling techniques to
} } this.
} }
} } Has anybody run across this problem and have thoughts they'd be willing
} } to share? The PI on this one has high hopes that we can put the
} } nano-rabbit of this tiny, little hat.
} }
} } Cheers,
} } Randy
} }
} } Randy Tindall
} } Senior EM Specialist
} } Electron Microscopy Core Facility---We Do Small Well!
} } W125 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-2227
} } Email: tindallr-at-missouri.edu
} } Web: http://www.emc.missouri.edu
} } On-line calendar:
} } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} } Week&NavType=Both&Type=TimePlan
} }
} }
} } ==============================Original Headers==============================
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} } 8, 23 -- Subject: Carbon nanos in tissue: SEM/TEM
} } 8, 23 -- Date: Wed, 6 Jun 2007 15:14:43 -0500
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} } 8, 23 -- Thread-Topic: Carbon nanos in tissue: SEM/TEM
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} } 8, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
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} ==============================Original Headers==============================
} 20, 17 -- From bfoster-at-mme1.com Wed Jun 6 15:34:13 2007
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} 20, 17 -- To: TindallR-at-missouri.edu, microscopy-at-microscopy.com
} 20, 17 -- From: Barbara Foster {bfoster-at-mme1.com}
} 20, 17 -- Subject: Re: [Microscopy] Carbon nanos in tissue: SEM/TEM
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==============================Original Headers==============================
8, 21 -- From dac-at-research.umass.edu Thu Jun 7 10:40:53 2007
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From: monroe-at-emcenter.msstate.edu
Date: Thu, 7 Jun 2007 10:52:13 -0500
Subject: [Microscopy] MSA 2007 Meeting: Request for Student and Other Volunteers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,

MSA 2007 is just a short 8 weeks away in beautiful Ft. Lauderdale, Florida !!!!

The reduced rate registration deadline is July 6, so please register early.

This is the second announcement for students who will attend the
Microscopy and Microanalysis Meeting in Ft. Lauderdale, Fl, August
5-9, 2007 and would like to apply for a Student Bursary to help
defray meeting costs. The complete description of the Bursary is
listed below, including the registration process.

In addition, technicians, post-docs, faculty and presenters may also
apply for a bursary in exchange for working to support the meeting.

The contact persons this year who will coordinate the student volunteers are;

Bill Monroe monroe-at-emcenter.msstate.edu
Amanda Lawrence alawrence-at-emcenter.msstate.edu
Mike Miller millem1-at-auburn.edu


STUDENT BURSARIES

The Microscopy Society of America values student members and
recognizes that they are the future of both the society and the field
of microscopy in general. MSA is therefore pleased to offer student
bur-saries of $200 to registered students. The most important purpose
of these bursaries is to encourage students to attend the annual
MSA/MAS Microscopy and Microanalysis meeting, where these young
sci-entists can meet and interact with the established microscopy
community as well as assisting with the meeting.

Each bursary recipient will be expected to work for a minimum of 20
hours during the meeting and/or at the pre-meeting weekend events. A
student may work up to an additional 20 hours, for a total of 40
hours. These extra hours will add to the bursary total at a rate of
$10 per hour. The maximum bursary will therefore be $400. The duties
will involve, but are not necessarily limited to, providing support
in symposia (helping with audio-visual needs, maintaining an
attendance count, and helping speakers set up for their
presentation), staffing the MSA Megabooth, and monitoring use of the
Internet Cafe. Applicants for the bursaries must be members of MSA or
MAS, and enrolled as students at a recognized educational
institution. All MSA or MAS student members are eligible for
bursaries, including those who are recipients of MSA Presidential
Scholars Awards and MAS Distinguished Scholar Awards. Eligible
students may apply for bursaries when registering for the conference
on the conference website, or at on-site registration. Bursaries are
limited and early application is encouraged.
How it works:

The registration form for the meeting will have a check box
indicating that the applicant is a registered student and is
requesting a bursary. The check box will have a note beside it
reminding the applicant that the bursary requires them to work at the
meeting. Students who have applied for bursaries will receive letters
from MSA explaining the conditions that they need to satisfy in order
to receive the bursaries. The tasks at the meeting will be allocated
by the Student Worker Organizers Sub-Committee of the Education
Committee. When students pick up their registration materials at the
meeting, they will receive assignment forms indicating the specific
tasks they are to perform, and the person(s) they need to contact in
order to carry out those tasks. Each stu-dent's assignment forms must
be signed by all of the contact persons listed, indicating that all
assigned tasks have been performed. Upon completion of assignments
and submission of the signed forms, the bursary check will be issued
by the appropriate representative at the registration desk.


Should you have questions concerning the process, please contact:

Bill Monroe monroe-at-emcenter.msstate.edu


--
Bill Monroe
Electron Microscope Center
103 Clay Lyle Entomology Building
Mississippi State University
Mississippi State, MS 39762
(662)-325-3019 Work
(662)-323-5246 Home
(662)-325-0246 Fax

==============================Original Headers==============================
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17, 15 -- To: "Microscopy-at-microscopy.com"-at-Ra.MsState.Edu
17, 15 -- From: "William A. Monroe" {monroe-at-emcenter.msstate.edu}
17, 15 -- Subject: MSA 2007 Meeting: Request for Student and Other Volunteers
17, 15 -- (Bursaries Available)
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From: bfoster-at-mme1.com
Date: Thu, 7 Jun 2007 11:11:00 -0500
Subject: [Microscopy] Re: Carbon nanos in tissue: SEM/TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dale,

To clarify: I am a consultant who has exposure to new technology
and, if you review my messages over the years, you will find a large
number of different technologies and vendors mentioned.

I did have a chance to work with NT-MDT (no longer)... and found that
AFM, like other technologies (ex: interferometry) was under-utilized.

Regarding giving a plug to one vendor: TOMO is unique. There is no
one else has integrated an AFM with an ultramicrotome for serial
sections... so there IS no one else to mention.

Hope this is helpful.

Barbara




At 10:44 AM 6/7/2007, you wrote:



} ----------------------------------------------------------------------------
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14, 17 -- To: dac-at-research.umass.edu, microscopy-at-microscopy.com
14, 17 -- From: Barbara Foster {bfoster-at-mme1.com}
14, 17 -- Subject: Re: [Microscopy] Carbon nanos in tissue: SEM/TEM
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From: oddioeng-at-aol.com
Date: Thu, 7 Jun 2007 11:14:25 -0500
Subject: [Microscopy] viaWWW: UPDATE of UC-4 Dewar Re-evacuation port

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
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Email: oddioeng-at-aol.com
Name: J. Allen Williams, Jr.

Organization: ORDELA, Inc.

Title-Subject: [Filtered] UPDATE of UC-4 Dewar Re-evacuation port

Question: Hello, I have discovered the answer to my question from yesterday, the ball in the evacuation port is the vacuum valve for the Dewar. If the Dewar is near atmosphere and if one needs to re-evacuate, you may use either a specified valve or what I did - (which is somewhat unorthodox) - connect your evaluation pump to the port and the ball
'magically levitates' from the o-ring seal. (This is also true of smaller LINDE Dewars). Therefore, the main seal of this type Dewar re-evacuation port seals the vacuum inside of the vacuum insulator by the atmospheric pressure on the ball and the o-ring seat.

I found this out by connecting my old Welch 1402 vacuum pump to the Dewar port and heard a click, and the click was a sound of the ball being sucked towards the vacuum pump. Mystery solvÈd. Thought I would let everyone know. It is amazing that atmospheric pressure can seal the vacuum in a liquid nitrogen/argon Dewar. Thanks for all the suggestions and hope this may help for reference in the future.

---------------------------------------------------------------------------


==============================Original Headers==============================
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8, 13 -- To: microscopy-at-microscopy.com
8, 13 -- From: oddioeng-at-aol.com (by way of MicroscopyListserver)
8, 13 -- Subject: viaWWW: UPDATE of UC-4 Dewar Re-evacuation port
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From: K.venner-at-ion.ucl.ac.uk
Date: Thu, 7 Jun 2007 11:15:05 -0500
Subject: [Microscopy] viaWWW: ultrathin sectioning of tissue containing nanotubes

Contents Retrieved from Microscopy Listserver Archives
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This Question/Comment was submitted to the Microscopy Listserver
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Email: K.venner-at-ion.ucl.ac.uk
Name: kerrie Venner

Organization: Institute of Neurology London UK

Title-Subject: [Filtered] ultrathin sectioning of tissue containing nanotubes

Question: Has anyone any experience of ultrathin sectioning of tissue containing nanotubes? Specifically the effect of nanotubes on diamond knives?

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: ecd10-at-psu.edu
Date: Thu, 7 Jun 2007 11:15:44 -0500
Subject: [Microscopy] viaWWW: Postdoctoral Position Open PSU

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html
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Email: ecd10-at-psu.edu
Name: Elizabeth Dickey

Organization: Pennsylvania State University

Title-Subject: [Filtered] Postdoctoral Position Open

Question: POST-DOCTORAL POSITION
in
Transmission Electron Microscopy
at
The Pennsylvania State University



A postdoctoral position is available in the area of transmission electron microscopy of amorphous dielectrics beginning July 1, 2007. The research project focuses on understanding structure and chemistry of amorphous metal oxides for capacitor applications. Through a variety of electron imaging, spectroscopy and diffraction techniques, including fluctuation electron microscopy, we aim to quantify structure and chemistry of amorphous metal-oxide layers to help establish processing/structure/property relationships for this class of materials. Most of the research will be conducted on a JEOL 2010F Field Emission TEM/STEM outfitted with an EDAX energy dispersive x-ray spectrometer (EDS), Gatan Enfina electron energy loss spectrometer (EELS), high-angle annular dark field STEM detector, and acquisition hardware and software for spectrum imaging. The ideal candidate for this position will have experience in HREM, STEM, EDS and EELS. The salary will be commensurate with qualifications and experience.

Please forward questions or send applications to:

Professor Elizabeth Dickey
Department of Materials Science and Engineering
The Pennsylvania State University
223 Materials Research Building
University Park, PA 16802
USA

tel: (814) 865-9067
FAX: (814) 865-2326
email: ecd10-at-psu.edu


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From: skooi-at-mit.edu
Date: Thu, 7 Jun 2007 11:16:50 -0500
Subject: [Microscopy] viaWWW: Research Specialist Position MIT

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Email: skooi-at-mit.edu
Name: Steven Kooi

Organization: ISN -at- MIT

Title-Subject: [Filtered] Research Specialist Position

Question: The Institute for Soldier Nanotechnologies (ISN) at MIT is looking for a research specialist to handle multiple responsibilities including transmission electron microscopy (TEM), scanning electron microscopy (SEM), focused ion beam (FIB), and atomic force microscopy (AFM). Primary responsibilities include providing introductory and more advanced training of users on electron microscopes, and assisting ISN researchers with sample preparation and characterization using electron and surface microscopy techniques. Other responsibilities may include daily operation, maintenance, and upkeep of several pieces of advanced nano-characterization instrumentation and related sample preparation tools; and serving as the primary contact with the equipment manufacturer's service personnel to quickly resolve any serious instrumentation issues.

The position announcement can be found at http://sh.webhire.com/servlet/av/jd?ai=631&ji=2025576&sn=I

Additional information on ISN is available at http://web.mit.edu/isn.

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From: TindallR-at-missouri.edu
Date: Thu, 7 Jun 2007 11:55:56 -0500
Subject: [Microscopy] Carbon nanos in tissue: SEM/TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree that keeping the list as "commercial free" as possible is a
good thing. However, I didn't really interpret Barbara's response to my
question as a commercial plug as much as pointing out a potentially
useful technology. In fact, I believe she pointed out that MME is no
longer affiliated with that vendor. Personally, I'll take any advice
that helps our lab help our clients with their research, whether from
consultants, vendors, or fellow techs.

I have had many vendors contact me off-list with suggestions involving
their products, when they thought their responses might be seen as too
commercial. To me that's a very courteous and professional response.
That said, I hope that those who are not formally affiliated with a
vendor won't hesitate to point out useful techniques or gizmos to the
list at large.

My personal opinion is that this particular response was not out of
line.

It's good to bring these issues up occasionally, so this is not a dig at
Dale, by any means.

Cheers,
Randy

-----Original Message-----
X-from: dac-at-research.umass.edu [mailto:dac-at-research.umass.edu]
Sent: Thursday, June 07, 2007 10:42 AM
To: Tindall, Randy D.

Dear Microscopists,

I know that vendors/vendor employees are active and involved in the MSA
and contribute greatly to the dialogs here. However, I personally feel
that this message reply is a bit over the top in commercializing the
List. Every question to the List can't be answered "you should try AFM"
and go on to plug the company and it's products. The response seems only
slightly related to the essence of the original question. How is AFM
going to visualize carbon nanotubes inside a cell that is inside a
tissue? This was a repeat of the response to recent technical questions
on vacuum metal shadowing technical problems. It is fine to bring
attention to alternate methods, but primarily we should be good
listeners and try to help people with the problem they have asked about.

Metal shadowing is a proven technique - with limitations, same as ALL
techniques - that people use routinely; one can scan relatively large
areas at high resolution for the best area. It is a method suitable to
the purpose. The DNA is already bulked up artificially by the
preparation method and the metal shadowing adds a known factor to that
size. But {visualizing} the DNA/plasmid is often the key, not the exact
molecular size. One vendor sent a link to an image of a 1um square scan
of DNA; the image was quite blurry, 2 nm/pixel resolution. I'm sure this
is a "display" result - the best they have. Finding a plasmid on a sheet
of carbon/mica for AFM scanning can't be as routine as the "traditional"

method of viewing the shadowed material in a TEM. It is a bit of
comparing apples and oranges.

So AFM is not the one answer to all questions, and please go light on
the commercial plugs. It seems that vendors were more respectful of this
"line" in the past.

Thanks for allowing my rant,

Dale Callaham



bfoster-at-mme1.com wrote:
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}
} Randy,
}
} This is another one of those "TOMO" applications.
}
} As mentioned earlier, I have a movie made by Dr.deWith of the Dutch
Polymer Institute (TU/E) showing the distribution of carbon nanotubes in
epoxy. Because the AFM uses local differences in elasticity, there is
none of the CNT in C-based tissue problem described below. Again,
although I am no longer involved in this project, I am happy to forward
a copy of the film to anyone who is interested. (It will come via
www.YouSendIt.com, because of the large file size).
}
} Dr. Anton Efimov of NT-MDT is the developer of this technique and is
usually willing to run samples, schedule permitting. See his email in
the CC: above.
}
} NTA is no longer NT-MDT's agent here in the US. If you are interested
in following this further, I recommend that you contact Dr. Yuri Bobrov,
who is now with ABeam (Dealer principle, Dr. Sergey Babin - see email
above).
}
} As always, if there are further questions, please don't hesitate to
call/email.
}
} (Note: MME is no longer involved with this vendor).
}
} Hope this was helpful,
} Barbara Foster, President
}
} Microscopy/Microscopy Education
} 313 S Jupiter Rd, Suite 100
} Allen, TX 75002
} P: 972-954-8011
} W: www.MicroscopyEducation.com
}
}
} MME is now scheduling customized, on-site courses through September.
Call us today for details.
}
} P. S.
} Need a good general reference or light microscopy text for next
semester? Call us today to learn more about "Optimizing LIght
Microscopy". Copies still available through MME... even for class-room
lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
}
}
}
}
} At 03:27 PM 6/6/2007, TindallR-at-missouri.edu wrote:
}
}
}
} } ---------------------------------------------------------------------
} } ------- The Microscopy ListServer -- CoSponsor: The Microscopy
} } Society of America To Subscribe/Unsubscribe --
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} }
} } Dear Listers,
} }
} } Every once in a while we get a request to image particles which have
} } been ingested by organisms, but this latest one seems especially
tricky.
} }
} } We are being asked to image carbon nanomaterials, such as nanowires,
} } and how they distribute themselves in the tissues and organs of
} } living organisms. My first search of the literature (ongoing, by the

} } way) indicates that I'm not the only one having trouble with this.
} } Aside from the obvious problems of differentiating 3-D structures in
} } nanometers-thick sections, there is the problem of seeing low-contrast

} } carbon in carbon-rich tissues.
} }
} } My first thoughts were that adding electron density to the carbon
} } nanothingys would make them easier to see, both in thin sections and
} } in fractured specimens for SEM. At least this could help localize
} } them, even if seeing their true shapes remains a problem. How to do
} } this is another thing. Probably the particles would have to be
} } augmented with metals before being released into the organisms'
} } environment, and that would be a task for the makers and users of the

} } nanoparticles. And would that affect their final distribution in the

} } creatures? From our end, I'm trying to think of how to adapt
} } immunolabeling techniques to this.
} }
} } Has anybody run across this problem and have thoughts they'd be
} } willing to share? The PI on this one has high hopes that we can put
} } the nano-rabbit of this tiny, little hat.
} }
} } Cheers,
} } Randy
} }
} } Randy Tindall
} } Senior EM Specialist
} } Electron Microscopy Core Facility---We Do Small Well!
} } W125 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-2227
} } Email: tindallr-at-missouri.edu
} } Web: http://www.emc.missouri.edu
} } On-line calendar:
} } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amou
} } nt=
} } Week&NavType=Both&Type=TimePlan
} }
} }
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From: jquinn-at-www.matscieng.sunysb.edu
Date: Thu, 7 Jun 2007 12:58:22 -0500
Subject: [Microscopy] Re: Re: Table Top SEM & High School Nano Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Caroline and everyone -

I'd like to convince all of you to encourage inductory imaging and
measurement in your "feeder" middle schools. It has been my
experience that too few students understand anything about
shape and size. Those who are unable to measure the diameter
and length of a broomstick are going to have a difficult time
on a nano-rod.

just my two cents............

JQuinn


PS...........OoO away........




} From mail-at-ns.microscopy.com Wed Jun 6 17:14:41 2007
} Date: Wed, 6 Jun 2007 16:17:10 -0500
} To: jquinn-at-www.matscieng.sunysb.edu
} From: schooley-at-mcn.org
} Reply-to: schooley-at-mcn.org
} X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com}
} Subject: [Microscopy] Re: Table Top SEM & High School Nano Course
} Errors-To: MicroscopyListSpamFilter-at-microscopy.com
} X-lewp: MicroscopyListSpam NAGS
}
}
}
}
} ----------------------------------------------------------------------------
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} ----------------------------------------------------------------------------
}
} I'm VERY interested. We need a database of dedicated people who are
} doing high school SEM, so that experience, problems, curricula, etc.
} can be shared. Will either of you be at M&M in Ft. Lauderdale?
} Please come to the MICRO booth so that we can try to organize
} something. Or send me an Email. If anyone else who is doing HS SEM,
} including those associated with the FEI & RJ Lee school SEM programs,
} is reading this, we need to talk to you too!
}
} What is my motive, other than trying to get you together? Project
} MICRO is MSA's outreach program for middle schools (see URL below).
} I'd like to convince all of you to encourage introductory LM in your
} "feeder" middle schools; SEM is an abrupt way to begin.
}
} Caroline
}
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} } ----------------------------------------------------------------------------
} }
} } Donovan,
} }
} } I have helped get a SEM put into my local high school. The science teacher
} } there now is able to offer 8 college credits to students that take his
} } advanced program related to this instrument. Mark would be able to talk to
} } you more about that aspect and how he integrates it into his curriculum. I
} } have cc'd him with this email so you'll have his email address. This is
} } still in the beginning stages so there is a lot yet to know and learn. But
} } I think it is an absolutely fabulous opportunity for learning...
} }
} } In the process of getting the SEM into my local high school, I have
} } learned a fair amount about SEM usage in high schools in the US and a bit
} } internationally. There are pockets of high schools around the USA that
} } have this kind of technology operating in high schools, but not a lot.
} } Germany has some high schools using it, apparently Japan has it
} } extensively used throughout their high school system.
} }
} } You are using Au and Ag particles. My experience indicates high school
} } students seem more interested in biological samples rather than materials.
} } It would be very useful if a materials curriculum could be developed that
} } would interest students at this level.
} }
} } Finding teachers and school systems that are open to this and have the
} } science background to make it work well, I think is a large hurdle. If a
} } solid curriculm could be put together that would work as a framework for
} } teachers to use, I think that would be very helpful.
} }
} } Schools are, justifiably, reluctant to use this technology as maintaining
} } these machines is quite expensive and most schools run on very tight
} } budgets. In the cases where I have seen SEM's working in high schools,
} } there has usually been someone that is knowledgable about the instruments
} } and can maintain them for the school pro-bono. These individuals have been
} } key in getting SEM's into high schools. (There may be a program in western
} } PA that's an exception to this, but I've not had any luck contacting
} } them.)
} }
} } Do send me your curriculum and further information about what you are
} } doing. I can give you more details of programs I've discovered if you
} } wish, but I don't think those details are of general interest to the list
} } (do correct me if I'm wrong).
} }
} } dj
} }
} } On Tue, 5 Jun 2007, donovan.leonard-at-gmail.com wrote:
} }
} } } Hello Listsers -
} } }
} } } Some days ago there was a question posed about the educational benefits
} } } of table top SEM and nano-scale samples. In a few weeks I'll post my
} } } experiences using a Hitachi TM-1000 table top SEM in a Nanotechnology
} } } course for high schoolers as part of the Duke University Talent
} } } Identification Program (TIP). More about the program can be found at
} } } 'www.tip.duke.edu'. I've chosen the TM-1000 to introduce the students
} } } to the micro and nanoscale, and we will be using the SEM, in addition to
} } } AFM and HRTEM to characterize Au and Ag nanoparticles the students
} } } synthesize as part of a experiential learning activity.
} } }
} } } In my opinion the table top SEM will allow the students hands-on
} } } experience with an electron microscope. Not that it will resolve atomic
} } } columns in nanoparticles, but as a way to introduce them to the scale of
} } } things. The intention is to let the students choose ANY samples they
} } } are interested in and let them discover the detail of features on the
} } } micro and nanoscale.
} } }
} } } If anyone has had experience integrating these microscopies into a high
} } } school course on nanotechnology I would be most interested in learning
} } } more about how it was accomplished and the outcomes. I would also be
} } } more than happy to share the syllabus created for the course if requested.
} } }
} } } Stay tuned, I'll post the student's data and feedback on the table top
} } } SEM, AFM and TEM.
} } }
} } Donovan
} --
} Caroline Schooley
} Project MICRO Coordinator
} Microscopy Society of America
} Box 117, 45301 Caspar Point Road
} Caspar, CA 95420
} Phone/FAX (707)964-9460
} Project MICRO: http://www.microscopy.org/ProjectMICRO
}
} ==============================Original Headers==============================
} 4, 19 -- From schooley-at-mcn.org Wed Jun 6 16:16:27 2007
} 4, 19 -- Received: from dns3.mcn.org (dns3.mcn.org [216.150.240.32])
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} 4, 19 -- Date: Wed, 6 Jun 2007 14:18:01 -0700
} 4, 19 -- To: dljones-at-bestweb.net, donovan.leonard-at-gmail.com
} 4, 19 -- From: Caroline Schooley {schooley-at-mcn.org}
} 4, 19 -- Subject: [Microscopy] Re: Table Top SEM & High School Nano Course
} 4, 19 -- Cc: Microscopy-at-MSA.Microscopy.Com, john_mackenzie-at-ncsu.edu,
} 4, 19 -- tpepper-at-iastate.edu, murphyjudy-at-comcast.net
} 4, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
} ==============================End of - Headers==============================
}


==============================Original Headers==============================
12, 12 -- From jquinn-at-www.matscieng.sunysb.edu Thu Jun 7 12:58:22 2007
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12, 12 -- Date: Thu, 7 Jun 2007 13:55:43 -0400
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12, 12 -- Message-Id: {200706071755.l57Hthw01791-at-www.matscieng.sunysb.edu}
12, 12 -- To: microscopy-at-microscopy.com
12, 12 -- Subject: Re: [Microscopy] Re: Table Top SEM & High School Nano Course
==============================End of - Headers==============================




From: Rosemary.White-at-csiro.au
Date: Thu, 7 Jun 2007 18:06:13 -0500
Subject: [Microscopy] fibre-optic probes/cameras?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

Are there any really tiny cameras about, or at least, fibre-optic
lenses/objectives? I ask because we have a couple of applications that
could use one. We're interested in roots growing down pre-existing soil
pores, and would like to watch what they're doing down there, either with a
moving imaging device of some kind, or a series of portholes to watch roots
growing past. Another group is screening different lines of cereals, in
particular, looking at development of the floral meristem. This object of
desire is enclosed in many leaves at ground level in a cereal plant, we'd
like to poke a small fibre-optic in to watch its development over 3-6 days,
rather than having to destroy many plants to follow the stages of
development.

I've had a rummage through numerous websites, and sent off some email
enquiries, but wondered if any on this list were aware of available
technology to do this.

cheers,
Rosemary

Dr Rosemary White rosemary.white-at-csiro.au
CSIRO Plant Industry ph. 61 (0)2-6246 5475
GPO Box 1600 fax. 61 (0)2-6246 5334
Canberra, ACT 2601
Australia

==============================Original Headers==============================
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5, 22 -- Subject: fibre-optic probes/cameras?
5, 22 -- From: Rosemary White {Rosemary.White-at-csiro.au}
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From: robby2-at-asu.edu
Date: Thu, 7 Jun 2007 18:34:42 -0500
Subject: [Microscopy] viaWWW: Position Available - ASU

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both robby2-at-asu.edu as well as the MIcroscopy Listserver
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Email: robby2-at-asu.edu
Name: Robert W. Roberson

Organization: Arizona State University

Title-Subject: [Filtered] Position Available

Question: Research Scientist

An individual is sought, at the PhD level, who is skilled in methods of light and electron microscopy. He or she will play a key bioimaging role in a recently funded project for the development of biofuels through the manipulation of the cyanobacterium Synechocystis sp. PCC 6803. The individual will interact closely with a group of investigators within the School of Life Science and Biodesign Institute at Arizona State University. Specifically, the individual will be responsible for designing and implementing suitable protocols for microscopic analysis of strains that have been genetically modified and/or grown under conditions that favor the over production of fatty acids. The successful candidate will be able to perform basic and advanced bioimaging protocols such as: confocal microscopy, cryofixation and freeze substitution, ultramicrotomy, standard TEM and SEM imaging and electron tomography, and immuno-cytochemistry at both the light and EM levels. The School of Life Sciences Bioimaging Facility (http://sols.asu.edu/klab/index.php; http://sols.asu.edu/lsem/index.php) and associated imaging facilities at Arizona State University maintain the equipment required to fulfill the goals of the project.


Contact:
Dr. Robert Roberson
School of Life Sciences
PO Box874501
Arizona State University
Tempe, AZ 85287-4501

Phone: 480-965-8618
Email: Robert. Roberson-at-asu.edu


---------------------------------------------------------------------------


==============================Original Headers==============================
12, 13 -- From zaluzec-at-microscopy.com Thu Jun 7 18:34:41 2007
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12, 13 -- From: robby2-at-asu.edu (by way of MicroscopyListserver)
12, 13 -- Subject: viaWWW: Position Available - ASU
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From: kraftpiano-at-gmail.com
Date: Thu, 7 Jun 2007 18:43:38 -0500
Subject: [Microscopy] Re: fibre-optic probes/cameras?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've used different sized boroscopes before, like the ones at
www.uxr.com (I have no affiliation with this company, I've just seen
their products in action before)

I would say that a small boroscope would be your best bet, as you can
port it out to video, and some come with a light source, but others
may need a separate light source. I know they make both a flexible
and a rigid boroscope, the rigid one being slightly less expensive.

What is the size diameter you are looking for?

--Justin A. Kraft

On 6/7/07, Rosemary.White-at-csiro.au {Rosemary.White-at-csiro.au} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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}
} Dear all,
}
} Are there any really tiny cameras about, or at least, fibre-optic
} lenses/objectives? I ask because we have a couple of applications that
} could use one. We're interested in roots growing down pre-existing soil
} pores, and would like to watch what they're doing down there, either with a
} moving imaging device of some kind, or a series of portholes to watch roots
} growing past. Another group is screening different lines of cereals, in
} particular, looking at development of the floral meristem. This object of
} desire is enclosed in many leaves at ground level in a cereal plant, we'd
} like to poke a small fibre-optic in to watch its development over 3-6 days,
} rather than having to destroy many plants to follow the stages of
} development.
}
} I've had a rummage through numerous websites, and sent off some email
} enquiries, but wondered if any on this list were aware of available
} technology to do this.
}
} cheers,
} Rosemary
}
} Dr Rosemary White rosemary.white-at-csiro.au
} CSIRO Plant Industry ph. 61 (0)2-6246 5475
} GPO Box 1600 fax. 61 (0)2-6246 5334
} Canberra, ACT 2601
} Australia
}
} ==============================Original Headers==============================
} 5, 22 -- From Rosemary.White-at-csiro.au Thu Jun 7 18:06:13 2007
} 5, 22 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au [150.229.7.37])
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} 5, 22 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0
} 5, 22 -- Date: Fri, 08 Jun 2007 09:09:44 +1000
} 5, 22 -- Subject: fibre-optic probes/cameras?
} 5, 22 -- From: Rosemary White {Rosemary.White-at-csiro.au}
} 5, 22 -- To: {microscopy-at-microscopy.com}
} 5, 22 -- Message-ID: {C28ECD58.1DFA3%Rosemary.White-at-csiro.au}
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==============================Original Headers==============================
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5, 29 -- Date: Thu, 7 Jun 2007 19:43:36 -0400
5, 29 -- From: "Justin Kraft" {kraftpiano-at-gmail.com}
5, 29 -- To: Rosemary.White-at-csiro.au
5, 29 -- Subject: Re: [Microscopy] fibre-optic probes/cameras?
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From: Rosey.VanDriel-at-csiro.au
Date: Thu, 7 Jun 2007 18:50:28 -0500
Subject: [Microscopy] fibre-optic probes/cameras?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Rosemary!
Have you contacted Optiscan? They have some pretty small endoscopic
microscopy devices which are really powerful. I'm sure they'd be as good
for your application as they are for animal work.
www.optiscan.com

Cheers,
Roseys

I have no affiliation with Optiscan, just impressed by the demos I've
seen


Rosemary van Driel
Electron Microscopy
New and Emerging Zoonotic Diseases
Australian Animal Health Laboratory
CSIRO Livestock Industries
Private Bag 24
Geelong Vic 3220
Australia

International Phone: +61 3 5227 5209
International Fax: +61 3 5227 5555
email: Rosey.VanDriel-at-csiro.au






-----Original Message-----
X-from: White, Rosemary (PI, Black Mountain)
Sent: Friday, 8 June 2007 09:08
To: Van Driel, Rosey (LI, Geelong)

Dear all,

Are there any really tiny cameras about, or at least, fibre-optic
lenses/objectives? I ask because we have a couple of applications that
could use one. We're interested in roots growing down pre-existing soil
pores, and would like to watch what they're doing down there, either
with a
moving imaging device of some kind, or a series of portholes to watch
roots
growing past. Another group is screening different lines of cereals, in
particular, looking at development of the floral meristem. This object
of
desire is enclosed in many leaves at ground level in a cereal plant,
we'd
like to poke a small fibre-optic in to watch its development over 3-6
days,
rather than having to destroy many plants to follow the stages of
development.

I've had a rummage through numerous websites, and sent off some email
enquiries, but wondered if any on this list were aware of available
technology to do this.

cheers,
Rosemary

Dr Rosemary White rosemary.white-at-csiro.au
CSIRO Plant Industry ph. 61 (0)2-6246 5475
GPO Box 1600 fax. 61 (0)2-6246 5334
Canberra, ACT 2601
Australia

==============================Original
Headers==============================
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5, 22 -- Subject: fibre-optic probes/cameras?
5, 22 -- From: Rosemary White {Rosemary.White-at-csiro.au}
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From: Rosemary.White-at-csiro.au
Date: Thu, 7 Jun 2007 19:22:22 -0500
Subject: [Microscopy] Re: fibre-optic probes/cameras?

Contents Retrieved from Microscopy Listserver Archives
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The diameter - as small as possible..... ideally 1mm or less, which I know
is smaller than most catheter imaging systems, for example. However, for
the root project, the boroscopes look pretty good.
And of course, I got more web results using fiber-optics rather than
fibre-optics.....the boroscope site didn¹t show up before...
thanks,
cheers,
Rosemary

} From: "Justin Kraft" {kraftpiano-at-gmail.com}
} Date: Thu, 7 Jun 2007 19:43:36 -0400
} To: Rosemary.White-at-csiro.au
} Cc: microscopy-at-microscopy.com
} Subject: Re: [Microscopy] fibre-optic probes/cameras?
}
} I've used different sized boroscopes before, like the ones at
} www.uxr.com (I have no affiliation with this company, I've just seen
} their products in action before)
}
} I would say that a small boroscope would be your best bet, as you can
} port it out to video, and some come with a light source, but others
} may need a separate light source. I know they make both a flexible
} and a rigid boroscope, the rigid one being slightly less expensive.
}
} What is the size diameter you are looking for?
}
} --Justin A. Kraft
}
} On 6/7/07, Rosemary.White-at-csiro.au {Rosemary.White-at-csiro.au} wrote:
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Dear all,
} }
} } Are there any really tiny cameras about, or at least, fibre-optic
} } lenses/objectives? I ask because we have a couple of applications that
} } could use one. We're interested in roots growing down pre-existing soil
} } pores, and would like to watch what they're doing down there, either with a
} } moving imaging device of some kind, or a series of portholes to watch roots
} } growing past. Another group is screening different lines of cereals, in
} } particular, looking at development of the floral meristem. This object of
} } desire is enclosed in many leaves at ground level in a cereal plant, we'd
} } like to poke a small fibre-optic in to watch its development over 3-6 days,
} } rather than having to destroy many plants to follow the stages of
} } development.
} }
} } I've had a rummage through numerous websites, and sent off some email
} } enquiries, but wondered if any on this list were aware of available
} } technology to do this.
} }
} } cheers,
} } Rosemary
} }
} } Dr Rosemary White rosemary.white-at-csiro.au
} } CSIRO Plant Industry ph. 61 (0)2-6246 5475
} } GPO Box 1600 fax. 61 (0)2-6246 5334
} } Canberra, ACT 2601
} } Australia
} }
} } ==============================Original Headers==============================
} } 5, 22 -- From Rosemary.White-at-csiro.au Thu Jun 7 18:06:13 2007
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} } 5, 22 -- Subject: fibre-optic probes/cameras?
} } 5, 22 -- From: Rosemary White {Rosemary.White-at-csiro.au}
} } 5, 22 -- To: {microscopy-at-microscopy.com}
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From: tstrixnerharvey-at-qmag.com.au
Date: Thu, 7 Jun 2007 19:36:01 -0500
Subject: [Microscopy] viaWWW: Purchasing an entry level SEM

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Email: tstrixnerharvey-at-qmag.com.au
Name: Tania Strixner-Harvey

Organization: Queensland Magnesia

Title-Subject: [Filtered] Purchasing an entry level SEM

Question: Good Morning Everyone,

I have been given the task of purchasing an "entry level" SEM capable of doing surface analaysis and elemental mapping of mineralogical samples.

Unfortunately may knowledge of SEM extends to sending samples away to a university and getting beautiful reports and photographs back.

What I need is an instrument able to do EDS measurements of light elements Z=11 and above. My samples need to be carbon coated and have an approximate minimum crystal size of 5 micron.

I want to use the instrument to look at gross crystal structures (at 5 micron) such as nicely layered or random etc.

Also I want to do elemental mapping of different products at larger crystal sizes (30-5000um).

I have a reasonable budget but not extensive.

I would appreciate some pointers.

Thank you kindly.

Kind Regards,


Tania Strixner-Harvey
Principal - Research and Development

Queensland Magnesia
246 Boundary Road
Parkhurst, 4702
Australia

Tel: 61 (0) 7 49 200 241
Fax: 61 (0) 7 49 361 380

Mobile: 0408 716 988


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From: alex.titkov-at-millenniumchem.com
Date: Thu, 7 Jun 2007 20:36:54 -0500
Subject: [Microscopy] Re: viaWWW: Purchasing an entry level SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tania,

X-from what you describe, you need not an "entry level" SEM, but a
conventional SEM (high vacuum, W filament) and a reasonable EDS with high
count rate and software to be able to do mapping.

I have JEOL 6400 with Oxford INCA EDS, it comfortably does what you
describe. The only complication is that the largest map it can do at a time
is about 6 mm. So, to acquire an elemental map of 5 mm crystals and have
several of them in the field of view you will also need a motorised stage
controlled by the EDS software. An alternative would be to collect several
maps and stitch them together.

You will also need to budget for some cutting/polishing equipment a carbon
coater.

Alex

==============
Alexander Titkov
Senior Scientist
Millennium Inorganic Chemicals
a Cristal Company
Lot 4 Old Coast Road
Australind WA 6233
AUSTRALIA





tstrixnerharvey-at-q
mag.com.au To: alex.titkov-at-millenniumchem.com
cc:
08/06/2007 08:38 Subject: [Microscopy] viaWWW: Purchasing an entry level SEM
AM
Please respond to
tstrixnerharvey









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Email: tstrixnerharvey-at-qmag.com.au
Name: Tania Strixner-Harvey

Organization: Queensland Magnesia

Title-Subject: [Filtered] Purchasing an entry level SEM

Question: Good Morning Everyone,

I have been given the task of purchasing an "entry level" SEM capable of
doing surface analaysis and elemental mapping of mineralogical samples.

Unfortunately may knowledge of SEM extends to sending samples away to a
university and getting beautiful reports and photographs back.

What I need is an instrument able to do EDS measurements of light elements
Z=11 and above. My samples need to be carbon coated and have an
approximate minimum crystal size of 5 micron.

I want to use the instrument to look at gross crystal structures (at 5
micron) such as nicely layered or random etc.

Also I want to do elemental mapping of different products at larger crystal
sizes (30-5000um).

I have a reasonable budget but not extensive.

I would appreciate some pointers.

Thank you kindly.

Kind Regards,


Tania Strixner-Harvey
Principal - Research and Development

Queensland Magnesia
246 Boundary Road
Parkhurst, 4702
Australia

Tel: 61 (0) 7 49 200 241
Fax: 61 (0) 7 49 361 380

Mobile: 0408 716 988


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From: ardnek2-at-att.net
Date: Thu, 7 Jun 2007 21:13:24 -0500
Subject: [Microscopy] AskAMicroscopist: Syringes with spores

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Name: Kendra Orr

Organization: ARDNEK TUTORING

Education: 6-8th Grade Middle School

Location: Fort Lauderdale, FL 33334

Title: Re: Syringes with spores

Question: This may seem like a stupid question. However, I want to be precise. May I order a spore syringe and it will spit out spores tiny enough to see on a microscope? I am sorry........I am doing a presentation and I usually use spores from real plants that I cultivate.

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From: gcouger-at-science-info.net
Date: Fri, 8 Jun 2007 02:50:52 -0500
Subject: [Microscopy] fibre-optic probes/cameras?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dr. White,

Do you or anyone else know if a large fiber optic strand of .25 to 1.0
mm can conduct an image that can be viewed with am 20x to 50x microscope
objective on the polished end of the fiber away form the subject in this
case roots.

I believe a lens could melted on the other end of a single fiber and
computer software could correct the distortions.

In a project such are yours I would think having a great number of low
cost lenses wold give much better coverage and allow you to cut years of
the time it takes to get results.

Gordon Couger
Stillwater OK
405 624 2855
Rosemary.White-at-csiro.au wrote:
}
} The diameter - as small as possible..... ideally 1mm or less, which I know
} is smaller than most catheter imaging systems, for example. However, for
} the root project, the boroscopes look pretty good.
} And of course, I got more web results using fiber-optics rather than
} fibre-optics.....the boroscope site didn¹t show up before...
} thanks,
} cheers,
} Rosemary
}
}
} } From: "Justin Kraft" {kraftpiano-at-gmail.com}
} } Date: Thu, 7 Jun 2007 19:43:36 -0400
} } To: Rosemary.White-at-csiro.au
} } Cc: microscopy-at-microscopy.com
} } Subject: Re: [Microscopy] fibre-optic probes/cameras?
} }
} } I've used different sized boroscopes before, like the ones at
} } www.uxr.com (I have no affiliation with this company, I've just seen
} } their products in action before)
} }
} } I would say that a small boroscope would be your best bet, as you can
} } port it out to video, and some come with a light source, but others
} } may need a separate light source. I know they make both a flexible
} } and a rigid boroscope, the rigid one being slightly less expensive.
} }
} } What is the size diameter you are looking for?
} }
} } --Justin A. Kraft
} }
} } On 6/7/07, Rosemary.White-at-csiro.au {Rosemary.White-at-csiro.au} wrote:
} }
} } }
} } } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ----------------------------------------------------------------------------
} } }
} } } Dear all,
} } }
} } } Are there any really tiny cameras about, or at least, fibre-optic
} } } lenses/objectives? I ask because we have a couple of applications that
} } } could use one. We're interested in roots growing down pre-existing soil
} } } pores, and would like to watch what they're doing down there, either with a
} } } moving imaging device of some kind, or a series of portholes to watch roots
} } } growing past. Another group is screening different lines of cereals, in
} } } particular, looking at development of the floral meristem. This object of
} } } desire is enclosed in many leaves at ground level in a cereal plant, we'd
} } } like to poke a small fibre-optic in to watch its development over 3-6 days,
} } } rather than having to destroy many plants to follow the stages of
} } } development.
} } }
} } } I've had a rummage through numerous websites, and sent off some email
} } } enquiries, but wondered if any on this list were aware of available
} } } technology to do this.
} } }
} } } cheers,
} } } Rosemary
} } }
} } } Dr Rosemary White rosemary.white-at-csiro.au
} } } CSIRO Plant Industry ph. 61 (0)2-6246 5475
} } } GPO Box 1600 fax. 61 (0)2-6246 5334
} } } Canberra, ACT 2601
} } } Australia
}




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From: nizets2-at-yahoo.com
Date: Fri, 8 Jun 2007 05:52:37 -0500
Subject: [Microscopy] Carbon nanos in tissue: SEM/TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Randy,

Been not involved in carbon nanotube research I dont
know exactly how dense they are under the beam of a
TEM. However I don't think you can compare the density
of the carbon present in a tissue with the density of
carbon in nanotubes. The difference MUST be visible.
I wouldn't modify the nanotubes themselves because you
will definitely modify their behaviour in an
uncontrolled manner.
Now here is what I would do:
I would incubate cell monolayers with the
nanoparticles and flat embed them (control=cells
incubated at 4°C, no uptake) after ferrocyanide-osmium
post-fixation. I am pretty sure the cells will
internalize the particles (take hepatocytes like HepG2
they are very effecient at uptaking and very nice to
show). After sectionning, I would try different
contrasting intensities and also without any
contrasting at all!
This way you can define your technical parameters to
obtain the best contrast possible in "control"
samples.

Finding these particles in organs is another story,
requiring mainly eternities of patience and
dedication.
But, if I may give my personal opinion, this is a very
interesting study and probably a very rewarding one.
Few have had the heart to start it.

Regards and good luck,

Stephane





____________________________________________________________________________________
Need a vacation? Get great deals
to amazing places on Yahoo! Travel.
http://travel.yahoo.com/

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From: maryflet-at-interchange.ubc.ca
Date: Fri, 8 Jun 2007 11:32:35 -0500
Subject: [Microscopy] viaWWW: Purchasing an entry level SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Tania,
You should consider a basic variable-pressure SEM. They are very good for
mineralogical work and have the added bonus of not requiring a carbon
coating. For what you want to do, the new, simple table-top SEMs, such as
the Hitachi TM-1000 or the FEI Phenom-Ed might be ideal, if they do EDS.
They are very fast, simple and low cost. They use BSE imaging, which shows
up the grains on polished minerals very well. Otherwise , a simple, low-cost
variable-pressure SEM will be your best bet. I didn't think that the
variable-pressure SEM's had much to offer materials engineering
applications, but we are using it all the time and finding new uses for it
every day.
BTW, all the modern EDS systems will do the elements Z=5 (boron) and above.
Regards,

Mary Fletcher (nee Mager)
Electron Microscopist
Materials Eng. UBC
#309 - 6350 Stores Road
Vancouver, B.C. V6T 1Z4
Canada
Tel: 604-822-5648
Fax: 604-822-3619
email: maryflet-at-interchange.ubc.ca


-----Original Message-----
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Email: tstrixnerharvey-at-qmag.com.au
Name: Tania Strixner-Harvey

Organization: Queensland Magnesia

Title-Subject: [Filtered] Purchasing an entry level SEM

Question: Good Morning Everyone,

I have been given the task of purchasing an "entry level" SEM capable of
doing surface analaysis and elemental mapping of mineralogical samples.

Unfortunately may knowledge of SEM extends to sending samples away to a
university and getting beautiful reports and photographs back.

What I need is an instrument able to do EDS measurements of light elements
Z=11 and above. My samples need to be carbon coated and have an approximate
minimum crystal size of 5 micron.

I want to use the instrument to look at gross crystal structures (at 5
micron) such as nicely layered or random etc.

Also I want to do elemental mapping of different products at larger crystal
sizes (30-5000um).

I have a reasonable budget but not extensive.

I would appreciate some pointers.

Thank you kindly.

Kind Regards,


Tania Strixner-Harvey
Principal - Research and Development

Queensland Magnesia
246 Boundary Road
Parkhurst, 4702
Australia

Tel: 61 (0) 7 49 200 241
Fax: 61 (0) 7 49 361 380

Mobile: 0408 716 988


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From: peggybryson-at-kemet.com
Date: Fri, 8 Jun 2007 17:27:46 -0500
Subject: [Microscopy] viaWWW: Voltage contrast

Contents Retrieved from Microscopy Listserver Archives
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Email: peggybryson-at-kemet.com
Name: Peggy Bryson

Organization: Kemet Electronics.com

Title-Subject: [Filtered] Voltage contrast

Question: I have a Cambridge SEM and I am trying to preform voltage contrast on a multi-layer ceramic capacitor. It has been well over 10 years since I've used VC and I must have forgotten something. I can blow them up, but can get them to light up. Does anyone have a guess on what I'm doing wrong. My parameters are 5 acceleration voltage. Power supply positive wire connected capacitor and negative connected to ground. SEM bias turned off. Thanks for any suggestions.

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From: zaluzec-at-microscopy.com
Date: Sat, 9 Jun 2007 11:49:38 -0500
Subject: [Microscopy] Administrivia: May 2007 Archives on-line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

The May Archives for the Microscopy Listserver are now on-line at

http://www.microscopy.com


In addition, you will notice I have written the output format
routines for the search engine. Hopefully the awkward long
bits of text which occassionaly caused the display to be skewed
will no longer cause problems.


Cheers,

Nestor
Your Friendly Neighborhood SysOp

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From: goodlg-at-matthey.com
Date: Mon, 11 Jun 2007 04:39:40 -0500
Subject: [Microscopy] SEM/TEM Position JM (U.K.)

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Electron Microscopist

Johnson Matthey PLC is a specialist chemicals company focused on its core skills in catalysts, precious metals and fine chemicals. The Technology Centre, based at Sonning Common (approximately 40 miles West of London), undertakes research work for the group.

A vacancy has arisen at the Technology Centre for an Electron Microscopist to join our team working with both Scanning and Transmission microscopes.

The successful candidate is expected to have experience in several of the following areas of expertise:

SEM and TEM sample preparation: (Coating; Ultramicrotomy; Cryo-sample preparation)
Electron Microscope technical knowledge: (FEG sources, vacuum systems; electronics; fault finding and diagnosis)
SEM and TEM Characterisation methods: (HRTEM; STEM; HAADF Imaging; EDX; EELS; Electron diffraction analysis)

The job is based on the analysis of a variety of samples from our research groups and operating divisions, thus prior exposure to multidisciplinary subjects as well as catalysis and materials would be an advantage.

The successful candidate will be educated to HNC/Degree level as a minimum.
Applications must be made in writing with full CV and current salary details to:
Georgie Floyd, Personnel Officer, Johnson Matthey Technology Centre, Blounts Court, Sonning Common, Reading, RG4 9NH or e-mail hrjmtc-at-matthey.com

Closing date for applications: Friday 6th July 2007






If the reader of this email is not the intended recipient(s), please be advised that any dissemination, distribution or copying of this information is strictly prohibited. Johnson Matthey PLC has its main place of business at 40-42 Hatton Garden, London (020 7269 8400).

Johnson Matthey Public Limited Company
Registered Office: 40-42 Hatton Garden, London EC1N 8EE
Registered in England No 33774

Whilst Johnson Matthey aims to keep its network free from viruses you should note that we are unable to scan certain emails, particularly if any part is encrypted or password-protected, and accordingly you are strongly advised to check this email and any attachments for viruses. The company shall NOT ACCEPT any liability with regard to computer viruses transferred by way of email.

Please note that your communication may be monitored in accordance with Johnson Matthey internal policy documentation.


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From: krensing-at-ucalgary.ca
Date: Mon, 11 Jun 2007 10:11:38 -0500
Subject: [Microscopy] aluminum evaporation

Contents Retrieved from Microscopy Listserver Archives
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Hello all,
I have been asked what the voltage and current conditions are for
evaporation of aluminum in a tungsten basket in a vacuum evaporator. Is
this sort of information available in a reference? I don't have much
experience with metal evaporation, so any advice would be appreciated.
Thanks,
Kim


--
Kim Rensing Ph.D.
Assistant Research Professor
Manager, Microscopy and Imaging Facility

University of Calgary
Health Sciences Centre
B129 - 3330 Hospital Drive NW
Calgary, AB, Canada T2N 4N1
403-220-3488
krensing-at-ucalgary.ca

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From: dale_batchelor-at-ncsu.edu
Date: Mon, 11 Jun 2007 12:26:52 -0500
Subject: [Microscopy] SEM: Peltier Cooled Stage

Contents Retrieved from Microscopy Listserver Archives
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Can anyone recommend or otherwise provide information on an
affordable(few thousand $) Peltier cold stage suitable for use in an SEM
level vacuum (we will modify to fit several instruments)? If you know
of suitable components, our machine shop can build or modify as needed.
Thanks,

Dale Batchelor, Ph.D.
Associate Director
Analytical Instrumentation Facility
N.C. State University
Monteith Research Center room 318A
Campus Box 7531
Raleigh, NC 27695
Office 919-515-3841
FAX 919-515-6965
E-Mail dale_batchelor-at-ncsu.edu
Website www.ncsu.edu/aif


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From: mdufraine-at-ebsciences.com
Date: Mon, 11 Jun 2007 12:43:04 -0500
Subject: [Microscopy] Re: SEM: Peltier Cooled Stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dale-

Emitech has a Peltier Cold Stage for SEM, the K25X, however, you'll pay
more than a few thousand dollars for the unit.
Energy Beam Sciences is the US Master Distributor for Emitech
Instruments, and we'd be happy to review your needs for this instrument
with you.

Regards,

Mike Dufraine
EM-Product Manager

dale_batchelor-at-ncsu.edu wrote:
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} Can anyone recommend or otherwise provide information on an
} affordable(few thousand $) Peltier cold stage suitable for use in an SEM
} level vacuum (we will modify to fit several instruments)? If you know
} of suitable components, our machine shop can build or modify as needed.
} Thanks,
}
} Dale Batchelor, Ph.D.
} Associate Director
} Analytical Instrumentation Facility
} N.C. State University
} Monteith Research Center room 318A
} Campus Box 7531
} Raleigh, NC 27695
} Office 919-515-3841
} FAX 919-515-6965
} E-Mail dale_batchelor-at-ncsu.edu
} Website www.ncsu.edu/aif
}
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} 3, 19 -- Subject: [Microscopy] SEM: Peltier Cooled Stage
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--
Michael F. Dufraine
EM - Product Manager
TEL 800-992-9037 X340
MDufraine-at-ebsciences.com
www.ebsciences.com


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From: gary-at-gaugler.com
Date: Mon, 11 Jun 2007 13:34:35 -0500
Subject: [Microscopy] Re: SEM: Peltier Cooled Stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I use a Deben XP stage and am very happy with it.
The XP does extended temperature at high end which
if not needed, would suggest a different model.

Deben is in the UK.

gary g.


At 09:28 AM 6/11/2007, you wrote:




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From: jd-at-laddresearch.com
Date: Mon, 11 Jun 2007 16:07:06 -0500
Subject: [Microscopy] Re: aluminum evaporation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Voltage and current requirements vary depending upon the volume of
aluminum you are evaporating. We coat substrates in the Ladd
evaporator by starting out with a very low voltage and adjusting it
higher till the aluminum starts to evaporate.

If you wish you can call Mike Bouchard at Ladd - 1-800-451-3406 to
discuss particulars. He has many years of experience in the
manufacture of the Ladd evaporator and substrate coating.

John Arnott

Disclaimer: Ladd sells electron microscopy supplies including a
vacuum evaporator, coated grids and substrates.

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: www.laddresearch.com

Telephone: 1-802-658-4961 (anywhere)
Toll Free 1-800-451-3406 (US)
Fax: 1-802-660-8859

e-mail: sales-at-laddresearch.com

At 11:18 AM 6/11/2007, you wrote:



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From: Joe.Williamson-at-fei.com
Date: Mon, 11 Jun 2007 16:28:37 -0500
Subject: [Microscopy] FW: FEI Job Opportunity Circuit Edit Applications Engineer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


FEI Company has a current opening for a Sr. Applications Engineer with a
background in front and back side circuit edit. This position is
located in Hillsboro, Oregon and would be responsible for providing
equipment testing, acceptance, demonstration, training, marketing, and
sales support to current and potential customers. If you are
interested, please follow the link to see the entire job description.
Feel free to apply directly online and I will see every resume that
comes in. We also have several other openings listed on our website as
well.

http://careers.fei.com/DetailFEI.asp?fei891-HBO

Thanks,


Joe Williamson
Corporate Recruiter
FEI Company
joe.williamson-at-fei.com
Direct Ph: 503-726-2639
Toll Free: 800-334-1403
Fax: 503-726-7509
www.fei.com



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From: sergei2-at-ornl.gov
Date: Mon, 11 Jun 2007 17:02:08 -0500
Subject: [Microscopy] MRS 2007 Fall Meeting - Symposium on Scanning Probe Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues
We would like to bring to your attention the symposium on Nanoscale
Phenomena in Functional Materials by Scanning Probe Microscopy, to be
held at the Materials Research Society 2007 Fall meeting in Boston, MA.
The symposium description is attached below and can also be accessed on
the MRS web-site (http://www.mrs.org/s_mrs/sec.asp?CID=8005&DID=192095).
The deadline for abstract submission is June 20.
Looking forward to seeing you in Boston
On behalf of the organizers
Sergei V. Kalinin




Symposium B: Nanoscale Phenomena in Functional Materials by Scanning
Probe Microscopy

The last decade has witnessed spectacular progress in the development
and applications of scanning probe microscopy (SPM)-based nanoscale
imaging techniques. The combination of high spatial resolution and
sensitivity to local electronic, optical, and mechanical properties
places these techniques among the most versatile tools for nanoscience,
biology, physics, and materials science. Atomic and electronic structure
of surfaces, vibrational excitations, energy flow, and local materials
properties on the molecular level has become accessible with the advent
of high-resolution SPMs. Electrostatic SPMs are being established as
powerful techniques for spatially resolved studies of electronic
transport on the nanometer level at electroactive interfaces and in
molecular electronic devices such as carbon nanotubes. Dynamic SPM modes
and scanning indentation techniques allow mechanical compliance and
surface energy to be investigated at the nanoscale with applications to
the emerging fields of nanotribology, nanofluidics, nanocomposites, and
NEMS. Novel optical imaging modes, such as tip-enhanced spectroscopy,
solid immersion microscopy, and apertureless scanning optical
microscopy, have joined the now-established near-field scanning optical
microscopy (NSOM), bringing the resolution of optical spectroscopy into
the nanoscale regime and complementing local electronic measurements of
materials with the STM family of instruments. These new measurement
techniques were necessitated by the growing need for materials
characterization on the nanoscale and have in turn led to the discovery
of new nanoscale phenomena. Finally, the SPM has been used to manipulate
and fabricate materials at the nanoscale.

It is the goal of this symposium to provide a multidisciplinary forum
for scanning-probe-based materials and nanoscience in order to
demonstrate the latest achievements in technique developments and
materials applications that have led to scientific discoveries. The
symposium will include two types of sessions: One will be dedicated to
the recent advances in technique development of interest to the
materials community and will bring together specialists in practical and
theoretical aspects of SPM imaging. The second will focus on specific
materials-related phenomena, including nanotubes and nanowires, quantum
dots, surfaces, interfaces, and biological systems studied by local
probe techniques.

The topics of the symposium will include, but not be limited to:

* Imaging, manipulation, and energy transfer on the atomic and
molecular level by atomic resolution NC-AFM and STM
* Local optical and electronic properties and excitations, e.g.,
plasmons measured with SPMs
* Defects, impurities, dopants, and transport in semiconductor
nanostructures, nanotubes, and nanowires
* Mechanics and electromechanics on the nanoscale by SPM and
nanoindentation
* Mechanical and voltage nanolithography and surface modification
* Energy flows and dissipation in materials, devices, and
nanostructures
* Transport in single-molecule devices and carbon nanotubes
* Imaging and characterization of ferroelectric materials
* Electronic properties of semiconductor heterostructures
* Imaging and characterization of biological systems
* Dynamics and imaging of polymers and soft materials

Invited speakers include: *Robert Carpick* (Univ. of Pennsylvania),
*Levent Degertekin* (Georgia Inst. of Technology), *Dennis Discher*
(Univ. of Pennsylvania), *Ricardo Garcia* (Univ. Madrid, Spain), *Franz
Giessibl* (Univ. Augsburg, Germany), *Venkat Gopalan* (Pennsylvania
State Univ.), *Jan Hoh* (Johns Hopkins Univ.), *Ernesto Joselevich*
(Weizmann Inst. of Science, Israel), *Maki Kawai* (RIKEN, Japan), *L.
Kuipers* (FOM, The Netherlands), *Alexander Malkin* (Lawrence Livermore
National Lab), *Lukas Novotny* (Univ. of Rochester), *E. Ward* *Plummer*
(Univ. of Tennessee/Oak Ridge National Lab), *V. Sandoghdar* (ETH
Zurich, Switzerland), *M. Tomitori* (JAIST, Japan), and *S. Wilks*
(Swansea Univ., United Kingdom).

*Symposium Organizers*

*Dawn** Bonnell*
University of Pennsylvania, Nano/Bio Interface Center, 3231 Walnut St.,
Philadelphia, PA 19104
Tel 215-898-6231, Fax 215-746-3204, bonnell-at-lrsm.upenn.edu
{mailto:bonnell-at-lrsm.upenn.edu}

*Sergei V. Kalinin*
Oak Ridge National Laboratory, Materials Sciences and Technology
Division and Center for Nanophase Materials Sciences, 1 Bethel Valley
Rd., Oak Ridge, TN 37831
Tel 865-241-0236, Fax 865-574-4143, sergei2-at-ornl.gov
{mailto:sergei2-at-ornl.gov}

*Sidney R. Cohen*
Weizmann Institute of Science, Surface Analysis Laboratory, Rehovot
76100 Israel
Tel 972-8-934-2703/3422, Fax 972-8-934-4137, sidney.cohen-at-weizmann.ac.il
{mailto:sidney.cohen-at-weizmann.ac.il}

*Richard E. Palmer*
University of Birmingham, School of Physics and Astronomy, Nanoscale
Physics Research Laboratory, Birmingham B15 2TT, United Kingdom
Tel 44-121-414-4653, Fax 44-121-414-7327, r.e.palmer-at-bham.ac.uk
{mailto:r.e.palmer-at-bham.ac.uk}


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From: L.Ryves-at-physics.usyd.edu.au
Date: Mon, 11 Jun 2007 19:25:06 -0500
Subject: [Microscopy] AskAMicroscopist: Beam voltage choice to minimise charging

Contents Retrieved from Microscopy Listserver Archives
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This Question was submitted to Ask-A-Microscopist by (L.Ryves-at-physics.usyd.edu.au)
from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, June 11, 2007 at 09:13:35
Remember to consider the Grade/Age of the student when considering the Question
---------------------------------------------------------------------------
Please reply to both L.Ryves-at-physics.usyd.edu.au as well as to the Microscopy Listserver
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Email: L.Ryves-at-physics.usyd.edu.au
Name: Luke Ryves

Organization: School of Physics / University of Sydney

Education: Graduate College

Location: Sydney, NSW, Australia

Title: Beam voltage choice to minimise charging

Question: Hi,

I'm just writing up my thesis and want to include some SEM images that were acquired for me ages ago. The samples were metal particles on SiO2 surfaces. At the time that we were taking the images I remember the microscopist explaining that the correct energy (2 to 5kV on a FEG SEM) can reduce charging, but I can't find a reference to this effect. Can you help me out by explaining how to choose the beam energy, and perhaps point me in the direction of some references?

---------------------------------------------------------------------------

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From: gary-at-gaugler.com
Date: Mon, 11 Jun 2007 19:45:16 -0500
Subject: [Microscopy] Re: AskAMicroscopist: Beam voltage choice to

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It is a fundamental principle that lower KV reduces
charging. Joy, Goldstein and Newbury, et. al. have
written about this factor. In practice, we see it
all the time.

The actual KV value depends on the specimen and the
ability of the SEM to image what you want to see.
I use 500V to 2KV on SiO2 and Hf oxides. This is
useful up to about 60KX with Zeiss Supra 55VP in HV
mode. Other systems will likely differ.

Hope this helps.

gary g.


At 04:26 PM 6/11/2007, you wrote:




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From: alex.titkov-at-millenniumchem.com
Date: Mon, 11 Jun 2007 19:53:38 -0500
Subject: [Microscopy] Re: aluminum evaporation

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Kim,

There is no set voltage/current to evaporate aluminium. A variable power
source is used to achieve this. A current is gradually increased until
aluminium melts and then a little bit more increase starts the evaporation.

We achieved good results using a tungsten wire with v-shaped bend on which
a small v-shaped piece of Al wire was hung.

Alex

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cc:
11/06/2007 11:15 Subject: [Microscopy] aluminum evaporation
PM
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Hello all,
I have been asked what the voltage and current conditions are for
evaporation of aluminum in a tungsten basket in a vacuum evaporator. Is
this sort of information available in a reference? I don't have much
experience with metal evaporation, so any advice would be appreciated.
Thanks,
Kim


--
Kim Rensing Ph.D.
Assistant Research Professor
Manager, Microscopy and Imaging Facility

University of Calgary
Health Sciences Centre
B129 - 3330 Hospital Drive NW
Calgary, AB, Canada T2N 4N1
403-220-3488
krensing-at-ucalgary.ca

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From: colijn.1-at-osu.edu
Date: Mon, 11 Jun 2007 20:41:01 -0500
Subject: [Microscopy] Re: AskAMicroscopist: Beam voltage choice to minimise

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Luke,

You can find information about varying the beam voltage in most any
edition of "Scanning Electron Microscopy and X-Ray Microanalysis"
(usually referred to as "SEMXM") by Goldstein, Newbury, Echlin, Joy,
Fiori, and Lifshin. Look for information on the production
efficiency of Secondary Electrons as a function of beam voltage. If
I remember correctly, the SE coefficient went above 1 roughly between
800V and 1.5kV for SiO2 (anyone want to correct me?).

Cheers,
Henk

At 08:26 PM 6/11/2007, you wrote:



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From: gary-at-gaugler.com
Date: Mon, 11 Jun 2007 20:48:41 -0500
Subject: [Microscopy] AskAMicroscopist: Beam voltage choice to

Contents Retrieved from Microscopy Listserver Archives
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That is quite probably true. That is why I use 1KV
and then 2KV. Beyond these values, specimens charge
too much to be of use....IMO.

Other ILDs besides SiO2 are quite interesting and
challenging. Of course, the gate oxides are another
challenge.

gary g.


At 05:42 PM 6/11/2007, you wrote:




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From: walck-at-southbaytech.com
Date: Mon, 11 Jun 2007 23:38:47 -0500
Subject: [Microscopy] Re: AskAMicroscopist: Beam voltage choice to minimise

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First, you want to use a fast scan and average the frames. Just use enough
frames in the average so that the updated image doesn't take too long. This
is a dynamic affect and helps a lot. Slow scans are more difficult to set
up the low voltage imaging. I can tell you that from personal experience on
an older non-digital Hitachi S-900. When you went to slow scan to shoot the
picture on Polaroid after using TV rates to set up a good charge balance
image, you got charging in the recorded image.

Secondly, you need to find the correct voltage for charge balance. This is
where the number of electrons (BSE and SE) is the same as incident number of
electrons, i.e. beam current. For most insulators, as mentioned before, the
voltage will be somewhere between 1 and 2 kV for a flat sample at zero tilt.
If I remember correctly, go to a mag of about 1000 X, stay there for a
little bit, then up the mag to 5000 X and stay there for about 20-30
seconds, then return quickly back to 1000 X. If you don't have charge
balance, you will see a box in the lower mag image. Look quickly, because
it will change. If the contrast of the box is bright relative to the low
mag area, then your box had charged negatively and you are above the balance
accelerating voltage. If the box was dark, then you were below the voltage
and you have to increase the voltage. If it doesn't change contrast, you
were JUST right. Eat your porridge and go take some nice pictures after
your nap.

Also note, that for tilted samples the voltage value will increase. For
example, on the JEOL Auger system, you could take uncoated insulators and do
analysis at a beam voltage of 3 kV if the sample was tilted to an angle of
about 70 degrees. This is because of the increase in both BSE and SE
signals at the higher tilt angles. Essentially, the voltage shifted from
about 1 kV at zero tilt to 3 kV at 70 degrees.

Hope this helps.


-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com



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From: bernard-at-berkeleyrc.com
Date: Tue, 12 Jun 2007 01:07:07 -0500
Subject: [Microscopy] Re: Specimen charging reference

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This is summarized in Goldstein's section 4.8.2., which is titled Charging.

Scanning Electron Microscopy and X-Ray Microanalysis, A Text for
Biologists, Materials Scientists, and Geologists, 2nd Ed., Goldstein,
J.I., et al., 1992.

--
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President, Mechanical Engineer, and Fire Scientist
Berkeley Research Company (BRC)
600 Addison Street
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From: nizets2-at-yahoo.com
Date: Tue, 12 Jun 2007 02:51:24 -0500
Subject: [Microscopy] Specimen charging reference

Contents Retrieved from Microscopy Listserver Archives
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After an internet search I found this book. Is this
the last edition of the same book?

Scanning Electron Microscopy and X-ray Microanalysis
Goldstein, J., Newbury, D.E., Joy, D.C., Lyman, C.E.,
Echlin, P., Lifshin, E., Sawyer, L.C., Michael, J.R.

3rd ed. 2003. Corr. 4th printing, 2007, 586 p., with 5
pg 4/C insert, Hardcover

Best regards,

Stephane

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} This is summarized in Goldstein's section 4.8.2.,
} which is titled Charging.
}
} Scanning Electron Microscopy and X-Ray
} Microanalysis, A Text for
} Biologists, Materials Scientists, and Geologists,
} 2nd Ed., Goldstein,
} J.I., et al., 1992.
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From: j.bilde-at-risoe.dk
Date: Tue, 12 Jun 2007 08:28:16 -0500
Subject: [Microscopy] AskAMicroscopist: Beam voltage choice to minimise charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A number of people have already explained the basic ideas and given you relevant references. I just wish to add that you can find experimental curves of secondary yield vs. acceleration voltage (including curves for SiO2) at the webaddress

http://www.mc-set.com/bse/

Best regards,

Jørgen B. Bilde-Sørensen
senior scientist, ph.d.
Phone direct +45 4677 5802
j.bilde-at-risoe.dk


Materials Research Department
Risø National Laboratory
Technical University of Denmark - DTU
Building 228, P.O. Box 49
DK-4000 Roskilde, Denmark
Tel +45 4677 5700
Fax +45 4677 5758
www.risoe.dk

X-from 1 January 2007, Risø National Laboratory, the Danish Institute for Food and Veterinary Research,
the Danish Institute for Fisheries Research, the Danish National Space Center and
the Danish Transport Research Institute have been merged with
the Technical University of Denmark (DTU) with DTU as the continuing unit.











-----Original Message-----
X-from: L.Ryves-at-physics.usyd.edu.au [mailto:L.Ryves-at-physics.usyd.edu.au]
Sent: Tuesday, June 12, 2007 2:30 AM
To: j.bilde-at-risoe.dk

This Question was submitted to Ask-A-Microscopist by (L.Ryves-at-physics.usyd.edu.au)
from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, June 11, 2007 at 09:13:35
Remember to consider the Grade/Age of the student when considering the Question
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Email: L.Ryves-at-physics.usyd.edu.au
Name: Luke Ryves

Organization: School of Physics / University of Sydney

Education: Graduate College

Location: Sydney, NSW, Australia

Title: Beam voltage choice to minimise charging

Question: Hi,

I'm just writing up my thesis and want to include some SEM images that were acquired for me ages ago. The samples were metal particles on SiO2 surfaces. At the time that we were taking the images I remember the microscopist explaining that the correct energy (2 to 5kV on a FEG SEM) can reduce charging, but I can't find a reference to this effect. Can you help me out by explaining how to choose the beam energy, and perhaps point me in the direction of some references?

---------------------------------------------------------------------------

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From: TindallR-at-missouri.edu
Date: Tue, 12 Jun 2007 08:43:12 -0500
Subject: [Microscopy] Carbon nanos in tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to everybody who replied to my query about imaging carbon
nanoparticles in tissue. I will prepare a summary of the replies soon
and post it for the list.

As usual, this list has been a great resource. Thanks again.

Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


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From: r-holdford-at-ti.com
Date: Tue, 12 Jun 2007 11:32:47 -0500
Subject: [Microscopy] Re: Specimen charging reference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stephanie: the 3rd edition is the most recent, but I think the 2nd
edition is more useful to the novice/intermediate user. The section
cited in the message by Dr. Cuzzillo is for the 2nd edition and is not
in the 3rd edition as its own section. The information may be scattered
around in Section 4 but I haven't sat down and looked for it.

Just an FYI: if you buy the 3rd edition as a used book, make sure it
has the CD with it.

nizets2-at-yahoo.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} After an internet search I found this book. Is this
} the last edition of the same book?
}
} Scanning Electron Microscopy and X-ray Microanalysis
} Goldstein, J., Newbury, D.E., Joy, D.C., Lyman, C.E.,
} Echlin, P., Lifshin, E., Sawyer, L.C., Michael, J.R.
}
} 3rd ed. 2003. Corr. 4th printing, 2007, 586 p., with 5
} pg 4/C insert, Hardcover
}
} Best regards,
}
} Stephane
}
} --- bernard-at-berkeleyrc.com wrote:
}
} ----------------------------------------------------------------------------
} } This is summarized in Goldstein's section 4.8.2.,
} } which is titled Charging.
} }
} } Scanning Electron Microscopy and X-Ray
} } Microanalysis, A Text for
} } Biologists, Materials Scientists, and Geologists,
} } 2nd Ed., Goldstein,
} } J.I., et al., 1992.
} }
} } --
} } Bernard R. Cuzzillo, Ph.D., P.E.
} } President, Mechanical Engineer, and Fire Scientist
} } Berkeley Research Company (BRC)
} } 600 Addison Street
} } Berkeley, CA 94710-1920
} } USA
} }
} } bernard-at-berkeleyrc.com
} }
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From: glenmac-at-u.washington.edu
Date: Wed, 13 Jun 2007 12:50:47 -0500
Subject: [Microscopy] MandM roommate site

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Is there a website for attendees looking to share hotel rooms for the
MandM meeting in Ft. Lauderdale?

thanks,
Glen


Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
glenmac-at-u.washington.edu

************************************************************************
******
The box said "Requires WindowsXP or better", so I bought a Macintosh.
************************************************************************
******



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From: zaluzec-at-aaem.amc.anl.gov
Date: Wed, 13 Jun 2007 13:04:40 -0500
Subject: [Microscopy] Re: This is no MandM roommate site

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sorry Glen, but there is no site like this setup by the Meeting.


Nestor
Your Friendly Neighborhood SysOp




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--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Lab
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Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
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The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================

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From: laable-at-solutia.com
Date: Thu, 14 Jun 2007 08:50:39 -0500
Subject: [Microscopy] SEM Ground

Contents Retrieved from Microscopy Listserver Archives
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To All Listers,

I have recently been having problems with my field emission scope lately at magnifications above 50KX. The image seems to swim and sparkles are seen and the focus boundaries. A slow scan image impossible to acquire. The ground is suspected. It is currently grounded to the building at the same location as all the rest of the analytical equipment. Our electricians are in the process of rerunning a separate ground for the scope. They are going to put an eight foot copper rod in the earth outside of my lab drill hole in the wall for wires to go through and attach it to the ground wires to the back of the scope. I was wondering how the rest of have run the grounds for your scopes. Any advice would be appreciated.

Lori Ables
Solutia, Inc.
laable-at-solutia.com


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From: wesaia-at-iastate.edu
Date: Thu, 14 Jun 2007 10:37:09 -0500
Subject: [Microscopy] SEM Ground

Contents Retrieved from Microscopy Listserver Archives
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You have not described the operating conditions or the samples. Has anything changed in those regards? Perhaps the matter is one of sample charging more so that instrumentation problems. I would certainly address those issues first with an application specialist or the list before resorting to the wiring change. That is not likely to be cheap.
 
If it is the ground, it sounds like the remedy is reasonable. It sounds like your people are on the right path.
 
Warren

________________________________________
X-from: laable-at-solutia.com [mailto:laable-at-solutia.com]
Sent: Thu 6/14/2007 8:51 AM
To: wesaia-at-iastate.edu

To All Listers,

I have recently been having problems with my field emission scope lately at magnifications above 50KX.  The image seems to swim and sparkles are seen and the focus boundaries.  A slow scan image impossible to acquire.  The ground is suspected.  It is currently grounded to the building at the same location as all the rest of the analytical equipment.  Our electricians are in the process of rerunning a separate ground for the scope.  They are going to put an eight foot copper rod in the earth outside of my lab drill hole in the wall for wires to go through and attach it to the ground wires to the back of the scope.  I was wondering how the rest of have run the grounds for your scopes.  Any advice would be appreciated.

Lori Ables
Solutia, Inc.
laable-at-solutia.com


This electronic mail message is intended exclusively for the individual or entity to which it is addressed.
This message, together with any attachment, may contain Solutia confidential and privileged information.
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Any unauthorized review, printing, retention, copying, disclosure, distribution, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited.  If you received this message in error, please immediately contact the sender by reply email and delete all copies of the material from any computer.  Thank you for your cooperation.



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From: gary-at-gaugler.com
Date: Thu, 14 Jun 2007 11:54:48 -0500
Subject: [Microscopy] Carbon "contamination"

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Greetings all--I am seeking input on what appears
to be Carbon contamination. Here is the situation.

Take a Pella 16111-9 stub out of bag and put on
holder. Take another stub out of bag and sputter
coat with Pd and put on holder. Do EDS on both.

un-coated:
wt% at%
C 7.5 15
O 4.5 7
Al 88 78

coated:
C 23 38
O 6.5 8
Al 71 53

SEM is Zeiss Supra 55VP with Edwards XDS10 dry
scroll pump and turbo. Coater is Denton Desk IV
with Edwards XDS5 and turbo.

The goal of using non-oil pumps was to reduce hydrocarbon
contamination. So, where is the C coming from? Nothing
has been done to the scroll pumps since new. There are
kits for repairing them but when is this necessary and
what would indicate that it be done? Would high C
be an indicator?

I'm stumped on this one.

Any ideas?

gary g.


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From: johnf-at-geology.wisc.edu
Date: Thu, 14 Jun 2007 12:30:30 -0500
Subject: [Microscopy] Re: Carbon "contamination"

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} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Can't help with the uncoated stub, but most of the "C Ka" you are
seeing is presumably from the Pd Mz line which is at 43.36 A (vs the
nominal 44.0 A for C Ka).

John
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From: david.knecht-at-uconn.edu
Date: Thu, 14 Jun 2007 12:40:46 -0500
Subject: [Microscopy] objective NA

Contents Retrieved from Microscopy Listserver Archives
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One can buy a 40-100x oil objective with an iris diaphram. What is
the theoretical difference in performance between imaging with a low
NA dry objective (.6NA for example) and an equivalent magnification,
high NA oil objective with the iris partially closed to reduce the NA
to .6? Should they be similar? Thanks- Dave


Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)



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From: bozzola-at-siu.edu
Date: Thu, 14 Jun 2007 13:11:28 -0500
Subject: [Microscopy] Re: Carbon "contamination"

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All,

The electricians have finished my ground and the result is amazing. I can now obtain a decent slow scan image at 500KX. Thanks to all who replied.

Lori

-----Original Message-----
X-from: Ables, Lori A
Sent: Thursday, June 14, 2007 8:50 AM
To: ' (Microscopy-at-Microscopy.Com)'

Gary,

You're pretty clever, Gary, and I'm sure you've already done this.
But......have you checked for any exposed wiring, gaskets or seals
near the target that might be degraded when the plasma is activated?
Are you using Argon gas to vent the system and as the source of
plasma? If so, how clean is the gas used to generate plasma?
Sometimes, gas cylinders contain traces of oil (as might the pressure
regulators). Check the threads for the presence of a lubricant.
Anyone else using the system? If so, they may have left some
contamination inside the chamber (like finger oils).

If you are still having problems, try putting a coated TEM grid
inside the chamber and examine it for traces of contamination.
Sometimes, "seeing" the contamination is a clue to where it may be
coming from.

Good luck.......

John B.

} Greetings all--I am seeking input on what appears
} to be Carbon contamination. Here is the situation.
}
} Take a Pella 16111-9 stub out of bag and put on
} holder. Take another stub out of bag and sputter
} coat with Pd and put on holder. Do EDS on both.
} The goal of using non-oil pumps was to reduce hydrocarbon
} contamination. So, where is the C coming from? Nothing
} has been done to the scroll pumps since new. There are
} kits for repairing them but when is this necessary and
} what would indicate that it be done? Would high C
} be an indicator?
}


--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

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From: donovan-at-uoregon.edu
Date: Thu, 14 Jun 2007 13:23:37 -0500
Subject: [Microscopy] Re: FW: SEM Ground

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Lori,
I am pleased that you saw such an improvement. Your original ground
connection must have been pretty bad!

Because of the importance of an electrically quiet and isolated
instrument ground, most instrument manufacturers won't install the
instrument unless one is available. For example FEI quotes a less
than 0.1 ohm resistance requirement and that doesn't even address the
electrical noise issue.

In the new integrated science complex (underground shared
instrumentation facilities) building that we are putting the
finishing touches on here at Univ of Oregon, we designed in separate
isolated, instrument grounds for each instrument. We had to negotiate
with the electrical inspector to get this through and it wasn't easy.
john


At 10:49 AM 6/14/2007, you wrote:

} All,
}
} The electricians have finished my ground and the result is
} amazing. I can now obtain a decent slow scan image at 500KX. Thanks
} to all who replied.
}
} Lori
}
} -----Original Message-----
} X-from: Ables, Lori A
} Sent: Thursday, June 14, 2007 8:50 AM
} To: ' (Microscopy-at-Microscopy.Com)'
} Subject: SEM Ground
}
} To All Listers,
}
} I have recently been having problems with my field emission scope
} lately at magnifications above 50KX. The image seems to swim and
} sparkles are seen and the focus boundaries. A slow scan image
} impossible to acquire. The ground is suspected. It is currently
} grounded to the building at the same location as all the rest of the
} analytical equipment. Our electricians are in the process of
} rerunning a separate ground for the scope. They are going to put an
} eight foot copper rod in the earth outside of my lab drill hole in
} the wall for wires to go through and attach it to the ground wires
} to the back of the scope. I was wondering how the rest of have run
} the grounds for your scopes. Any advice would be appreciated.
}
} Lori Ables
} Solutia, Inc.
} laable-at-solutia.com
}
}
} This electronic mail message is intended exclusively for the
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From: ph2-at-sprynet.com
Date: Thu, 14 Jun 2007 13:47:28 -0500
Subject: [Microscopy] objective NA

Contents Retrieved from Microscopy Listserver Archives
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Using basic theory the resolution should be the same [d =
(Wavelength)/(2*NA)]. The fourier plane would be slightly different but not
sure of the effects.

The depth of field should be (theoretically) better with an oil/water
immersion stopped down. I haven't tested this personally.

Practically, I've noticed that there is less noise (scattered) light with
both my oil and water immersion objectives.

Tony

......................................................................
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-----Original Message-----
X-from: david.knecht-at-uconn.edu [mailto:david.knecht-at-uconn.edu]
Sent: Thursday, June 14, 2007 1:45 PM
To: ph2-at-sprynet.com

One can buy a 40-100x oil objective with an iris diaphram. What is
the theoretical difference in performance between imaging with a low
NA dry objective (.6NA for example) and an equivalent magnification,
high NA oil objective with the iris partially closed to reduce the NA
to .6? Should they be similar? Thanks- Dave


Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)



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From: gary-at-gaugler.com
Date: Thu, 14 Jun 2007 13:52:46 -0500
Subject: [Microscopy] Carbon "contamination"

Contents Retrieved from Microscopy Listserver Archives
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Thanks for the reply and to the others who have replied.

More data.

Gas is industrial welding Ar run through a Matheson
molecular sieve (to dry and filter). SEM chamber
uses industrial N2 also run through a molecular sieve.

Specimens are put in SEM chamber via Fjeld M-100
specimen load lock. This unit is pumped with small
oil pump and turbo. Main SEM door is rarely opened.
I have to check load lock pumps to see if it really
is an oil roughing pump. I thought I got a dry unit
for this too.

Pd target is from Refining Systems Las Vegas and is
99.5% pure. Trace elements do not include C. Coater
is only used by myself. Chamber of coater is stainless
steel (so it seems--but metal nevertheless). Only visible
seal is the L ring rubber, or whatever, at the top.

This C issue just came up while trying to quant TaN on
Si. It showed C that should not be there. So now I wonder
about all spectra work and quants that include C.
I can't think of a way to narrow down where the C is
coming from and how to negate it. I will try cleaning
the stubs and also try other stub types.

Exactly what are you saying about the TEM grid? The
procedure is not clear to me. Are you saying I should
coat it with Pd? Then what? I have STEM but not TEM.

gary g.





At 10:13 AM 6/14/2007, you wrote:
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From: stefan.diller-at-t-online.de
Date: Thu, 14 Jun 2007 14:15:13 -0500
Subject: [Microscopy] EDS problem - fluorescence ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
I did some EDS spectra on small steel particles. Specimen is uncoated on a
adhesive C-tab.
Energy had been 15 KV. I used a Roentec SLEW window detector.
See images and spectra at www.elektronenmikroskopie.info/eds

Is there any other explaination for the titan peak to show up rather than
it really is there in the specimen?
Is there any possibility for fluorescense?
Sorry in advance if this question is too simple for the list. I am not very
experienced in interpreting eds spectra...

Best regards,
Stefan


----------------------------------------------------------------------------
-----------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49 - 931 - 7848700 Phone
++49 - 931 - 7848701 Fax
++49 - 175 - 717 70 51 Cell-Phone

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
Anfahrt: http://mail.map24.com/stefan.diller
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From: wesaia-at-iastate.edu
Date: Thu, 14 Jun 2007 14:29:03 -0500
Subject: [Microscopy] EDS problem - fluorescence ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My first impression is that the titanium is real. It would be a little
surprising for a regular stainless steel, but I see you also have cobalt
peaks present. The peak around 7 kV is too intense to be only Fe K-beta.
Co K-alpha is at 6.93 kV. You have a somewhat unusual specimen and Ti
might be in order.

I don't know your x-ray system. I would highly recommend deconvoluting
with the elements you know to be present and then examining the
residuals from the fit. (Hopefully your system can show you the
residuals. They are very helpful.) See what peaks or portions thereof
are unaccounted for. I have had several different flavors of EDS systems
over the years. They generally do a fair job of deconvolution _if_ you
give them the right elements to begin with. For example, if I have a
small mess around 2.3 kV and I let the EDS work with both S-K and Mo-L
and Pb-M, it will generally give me a fair idea of which element is
present if I have counted enough to well define my peaks.

The corollary is if you give the wrong elements or let the EDS pick the
wrong elements on its own, you can get some quite screwy answers. Mo can
turn into S. Ba can turn into Ti and Ag can turn into Ar.

Warren

-----Original Message-----
X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de]
Sent: Thursday, June 14, 2007 2:16 PM
To: wesaia-at-iastate.edu

Dear All,
I did some EDS spectra on small steel particles. Specimen is uncoated on
a
adhesive C-tab.
Energy had been 15 KV. I used a Roentec SLEW window detector.
See images and spectra at www.elektronenmikroskopie.info/eds

Is there any other explaination for the titan peak to show up rather
than
it really is there in the specimen?
Is there any possibility for fluorescense?
Sorry in advance if this question is too simple for the list. I am not
very
experienced in interpreting eds spectra...

Best regards,
Stefan


------------------------------------------------------------------------
----
-----------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49 - 931 - 7848700 Phone
++49 - 931 - 7848701 Fax
++49 - 175 - 717 70 51 Cell-Phone

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
Anfahrt: http://mail.map24.com/stefan.diller
------------------------------------------------------------------------
----
-----------------------------------------



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From: jquinn-at-www.matscieng.sunysb.edu
Date: Thu, 14 Jun 2007 14:57:15 -0500
Subject: [Microscopy] re: Pd two main M lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary and company

http://www.matscieng.sunysb.edu/temp/gg/

The link will vanish is a few weeks.

The three spectra are calculations.

The first is for C-K and O-K with 100cts.

The second is for Pd-M with 100cts.

The third is them combined.

You would see the same with a real sample.

HPD from EDAX works okay at low energy, but
the labelling is not as sharp.

regards,

Jim

PS: OoO away.......

} From mail-at-ns.microscopy.com Thu Jun 14 12:52:28 2007
} Date: Thu, 14 Jun 2007 11:55:33 -0500
} To: jquinn-at-www.matscieng.sunysb.edu
} From: gary-at-gaugler.com
} Reply-to: gary-at-gaugler.com
} X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com}
} Subject: [Microscopy] Carbon "contamination"
} Errors-To: MicroscopyListSpamFilter-at-microscopy.com
} X-lewp: MicroscopyListSpam NAGS
}
}
}
}
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} Greetings all--I am seeking input on what appears
} to be Carbon contamination. Here is the situation.
}
} Take a Pella 16111-9 stub out of bag and put on
} holder. Take another stub out of bag and sputter
} coat with Pd and put on holder. Do EDS on both.
}
} un-coated:
} wt% at%
} C 7.5 15
} O 4.5 7
} Al 88 78
}
} coated:
} C 23 38
} O 6.5 8
} Al 71 53
}
} SEM is Zeiss Supra 55VP with Edwards XDS10 dry
} scroll pump and turbo. Coater is Denton Desk IV
} with Edwards XDS5 and turbo.
}
} The goal of using non-oil pumps was to reduce hydrocarbon
} contamination. So, where is the C coming from? Nothing
} has been done to the scroll pumps since new. There are
} kits for repairing them but when is this necessary and
} what would indicate that it be done? Would high C
} be an indicator?
}
} I'm stumped on this one.
}
} Any ideas?
}
} gary g.
}
}
} ==============================Original Headers==============================
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13, 12 -- Subject: re: Pd two main M lines
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From: David.R.Hull-at-nasa.gov
Date: Thu, 14 Jun 2007 15:21:18 -0500
Subject: [Microscopy] Re: SEM Ground

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lori,

Do you observe the same effect with all detectors, (ie. A back scattered
electron detector)? You might be having a problem with your secondary
electron detector.

On 6/14/07 9:51 AM, "laable-at-solutia.com" {laable-at-solutia.com} wrote:

}
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} To All Listers,
}
} I have recently been having problems with my field emission scope lately at
} magnifications above 50KX. The image seems to swim and sparkles are seen and
} the focus boundaries. A slow scan image impossible to acquire. The ground is
} suspected. It is currently grounded to the building at the same location as
} all the rest of the analytical equipment. Our electricians are in the process
} of rerunning a separate ground for the scope. They are going to put an eight
} foot copper rod in the earth outside of my lab drill hole in the wall for
} wires to go through and attach it to the ground wires to the back of the
} scope. I was wondering how the rest of have run the grounds for your scopes.
} Any advice would be appreciated.
}
} Lori Ables
} Solutia, Inc.
} laable-at-solutia.com
}
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From: donovan-at-uoregon.edu
Date: Thu, 14 Jun 2007 15:22:27 -0500
Subject: [Microscopy] Instrument Engineer Position at the University of Oregon

Contents Retrieved from Microscopy Listserver Archives
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Electron/Ion Beam Instrument Engineer
The University of Oregon's Center for Advanced Materials
Characterization in Oregon (CAMCOR) is seeking applications for a
full time staff position to begin September 2007. A strong background
in maintaining, trouble shooting and upgrading electron/ion beam
instruments and associated high voltage, vacuum, mechanical and
electrical systems is required. Experience with x-ray diffraction
instrumentation is also desirable. Salary range $60K-90K
commensurate with experience.

This position will be located in the new Lorry Lokey Integrated
Science Laboratory, a state of the art nano and micro science
analytical instrument facility designed specifically for exceptional
nano-science performance. It will house the latest electron, ion and
x-ray beam instrumentation available including a Zeiss Ultra TFEM,
FEI Quanta 200 E-SEM, Cameca SX50 and SX100 microprobes, Ion-TOF
SIMS, Philips Auger Spectrometer, Philips TEM, Kratos XPS and various
assorted coaters, etchers, and other vacuum deposition systems.

The successful candidate with have a BS in a beam microscopy related
field and an extensive background in instrument field service with
significant practical experience troubleshooting high vacuum electron
and ion beam instrumentation at both the system and PC board levels.
Must be able to read and understand schematics for electronic
circuits and systems. The successful applicant will be involved in
modifying/improving instrumentation capabilities to enable the
equipment to more fully support unique research needs and will be
expected to work intimately with the scientific staff and research
faculty. We seek candidates with a demonstrated commitment to working
effectively with students, faculty and staff from diverse backgrounds.

Interested persons should send a resume with a detailed description
of work experience and skills, and arrange for two letters of
recommendation to be sent to: CAMCOR Instrument Engineer Search
Committee, 1253 University of Oregon, Eugene, OR, 97403-1253. To be
assured of full consideration, application materials must be received
by July 31, 2007, but the search will remain open until the position
is filled. For further information, contact John Donovan (donovan-at-uoregon.edu).

University of Oregon is an AA/EEO employer committed to cultural diversity.


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From: Jane.LaGoy-at-Bodycote.com
Date: Thu, 14 Jun 2007 15:36:47 -0500
Subject: [Microscopy] Materials Engineer position opening

Contents Retrieved from Microscopy Listserver Archives
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Materials Engineer job opening -- Bodycote HIP (Hot Isostatic Pressing)
in Andover, Massachusetts, has an immediate opening for an entry-level
B.S. or M.S. materials or metallurgical engineer for our Technical
Services group. The ideal candidate will have a strong interest and/or
experience in metallography, microscopy and failure analysis. He/she
will interact with both internal and external customers, so good
communication skills are a must. A solid background in thermodynamics
and physical metallurgy is also desired. Powder metallurgy,
heat-treating and/or foundry knowledge is also a plus.

Andover, MA, is the North American headquarters of the Bodycote HIP
group. Bodycote HIP is part of the global metals processing
corporation, Bodycote International plc, the industry leader in both HIP
and heat-treating services with annual sales in excess of $800 million.
The Technical Services team in Andover supports all thermal processing
plants throughout North America. Long-term opportunities for
advancement within the corporation are possible. Bodycote is an equal
opportunity employer.

To submit a resume, or for further information, contact: Ms. Kathy
Barnett, Human Resources Mgr., Bodycote HIP, Inc., 155 River St.,
Andover, MA 01810, email: jobs-at-bodycote.com.



This Email and all attachments hereto, if any, are covered by the provisions of the U.S. Electronic Communications Privacy Act. Additionally, all contents of this communication and all attachments hereto shall be deemed to be confidential and the contents thereof are proprietary to Bodycote Thermal Processing, Inc. ("Bodycote"). If this communication has been sent to you in error, please reply to the sender that you received it and then delete the message immediately. Any dissemination, distribution, copying or reproduction of this message other than by its intended recipient is strictly prohibited.



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From: gary-at-gaugler.com
Date: Thu, 14 Jun 2007 15:53:30 -0500
Subject: [Microscopy] Pd two main M lines

Contents Retrieved from Microscopy Listserver Archives
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Check out the spectra at

http://www.gaugler.com/coatedstub.pdf

The only possible overlap with C and Pd is
at Pd Mz=0.286KeV. But at 5KV beam, I would
expect this Pd peak to be quite low.

Then, it still does not explain why I see
C on an un-coated stub. The suggestion to
clean some is good and I will do that. I
checked the specimen holder and it is loaded
with organics and C--likely from machining.

I can also try X-Checker and do Cu at 5KV
and then try a Si die. This would eliminate
any low KeV peaks near C. If I still see C,
then...??? The low KV is necessary to analyze
TaN since the N is oddly bound to the Ta. Different
(higher) KV gives different results. I've done
routing EDS on TiN and it is quite different.

A possible coating factor is the Ar cylinder. It
is steel and is about seven years old. I wonder if
over time, it oozes C into the Ar? A 2,000psi
tank seems to last forever.

gary g.


At 11:58 AM 6/14/2007, you wrote:




} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


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14, 20 -- From: Gary Gaugler {gary-at-gaugler.com}
14, 20 -- Subject: Re: [Microscopy] re: Pd two main M lines
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From: jquinn-at-www.matscieng.sunysb.edu
Date: Thu, 14 Jun 2007 16:21:21 -0500
Subject: [Microscopy] Pd two main M lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

G^2

The thin Pd coating may enhance the M peaks over the L peaks

Run a simulation with WinXRay at 5kV.
See fourth image at this URL:
http://www.matscieng.sunysb.edu/temp/gg/

You will see two Pd-M peaks are nearly the size of
the Pd-Lb peak. You can tweak the thickness to
change the M/L ratio.

Also, with the weak signal, you may be seeing
secondary emission from your polymer window.

Also, carbon is everywhere. Fact of life.
HF etch a silicon a chip from a silicon wafer.
Take a spectrum. That will be your low limit
on cleanliness of carbon (not oxygen). You could
also Shirake etch the Si chip.

JQ


} From gary-at-gaugler.com Thu Jun 14 16:50:19 2007
} X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9
} Date: Thu, 14 Jun 2007 13:53:28 -0800
} To: jquinn-at-www.matscieng.sunysb.edu
} From: Gary Gaugler {gary-at-gaugler.com}
} Subject: Re: [Microscopy] re: Pd two main M lines
} Cc: MSA listserver {microscopy-at-microscopy.com}
} In-Reply-To: {200706141958.l5EJwkaB019860-at-ns.microscopy.com}
} References: {200706141958.l5EJwkaB019860-at-ns.microscopy.com}
} Mime-Version: 1.0
} Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-46F525F
}
} Check out the spectra at
}
} http://www.gaugler.com/coatedstub.pdf
}
} The only possible overlap with C and Pd is
} at Pd Mz=0.286KeV. But at 5KV beam, I would
} expect this Pd peak to be quite low.
}
} Then, it still does not explain why I see
} C on an un-coated stub. The suggestion to
} clean some is good and I will do that. I
} checked the specimen holder and it is loaded
} with organics and C--likely from machining.
}
} I can also try X-Checker and do Cu at 5KV
} and then try a Si die. This would eliminate
} any low KeV peaks near C. If I still see C,
} then...??? The low KV is necessary to analyze
} TaN since the N is oddly bound to the Ta. Different
} (higher) KV gives different results. I've done
} routing EDS on TiN and it is quite different.
}
} A possible coating factor is the Ar cylinder. It
} is steel and is about seven years old. I wonder if
} over time, it oozes C into the Ar? A 2,000psi
} tank seems to last forever.
}
} gary g.
}
}
} At 11:58 AM 6/14/2007, you wrote:
}
}
}
}
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Gary and company
} }
} } http://www.matscieng.sunysb.edu/temp/gg/
} }
} } The link will vanish is a few weeks.
} }
} } The three spectra are calculations.
} }
} } The first is for C-K and O-K with 100cts.
} }
} } The second is for Pd-M with 100cts.
} }
} } The third is them combined.
} }
} } You would see the same with a real sample.
} }
} } HPD from EDAX works okay at low energy, but
} } the labelling is not as sharp.
} }
} } regards,
} }
} } Jim
} }
} } PS: OoO away.......
} }
} } } From mail-at-ns.microscopy.com Thu Jun 14 12:52:28 2007
} } } Date: Thu, 14 Jun 2007 11:55:33 -0500
} } } To: jquinn-at-www.matscieng.sunysb.edu
} } } From: gary-at-gaugler.com
} } } Reply-to: gary-at-gaugler.com
} } } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com}
} } } Subject: [Microscopy] Carbon "contamination"
} } } Errors-To: MicroscopyListSpamFilter-at-microscopy.com
} } } X-lewp: MicroscopyListSpam NAGS
} } }
} } }
} } }
} } }
} } }
} } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } ----------------------------------------------------------------------------
} } }
} } } Greetings all--I am seeking input on what appears
} } } to be Carbon contamination. Here is the situation.
} } }
} } } Take a Pella 16111-9 stub out of bag and put on
} } } holder. Take another stub out of bag and sputter
} } } coat with Pd and put on holder. Do EDS on both.
} } }
} } } un-coated:
} } } wt% at%
} } } C 7.5 15
} } } O 4.5 7
} } } Al 88 78
} } }
} } } coated:
} } } C 23 38
} } } O 6.5 8
} } } Al 71 53
} } }
} } } SEM is Zeiss Supra 55VP with Edwards XDS10 dry
} } } scroll pump and turbo. Coater is Denton Desk IV
} } } with Edwards XDS5 and turbo.
} } }
} } } The goal of using non-oil pumps was to reduce hydrocarbon
} } } contamination. So, where is the C coming from? Nothing
} } } has been done to the scroll pumps since new. There are
} } } kits for repairing them but when is this necessary and
} } } what would indicate that it be done? Would high C
} } } be an indicator?
} } }
} } } I'm stumped on this one.
} } }
} } } Any ideas?
} } }
} } } gary g.
} }
}



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From: frah0010-at-umn.edu
Date: Thu, 14 Jun 2007 18:58:38 -0500
Subject: [Microscopy] Buehler IsoMet

Contents Retrieved from Microscopy Listserver Archives
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Microscopists,

Our lab has a Buehler IsoMet slow saw (model #11-1180, apparently
1980s vintage) that has ceased functioning. I've tracked the problem
down to broken gear teeth on the shaft perpendicular to the motor's
drive shaft. Does anyone else have a dead or unused IsoMet that they
wouldn't mind someone cannibalizing? We can pay shipping and/or
modestly for the equipment/parts. We also don't have the
instructions or specifications anymore. If someone else does and
there are good part descriptions, I'd be interested in a fax or scan
of that page to help track down a replacement part. Thanks in advance!

Cheers,
Ellery

--------------------
Ellery E. Frahm
Research Fellow & Manager
Electron Microprobe Laboratory
University of Minnesota - Twin Cities
Department of Geology & Geophysics
Lab Website: http://probelab.geo.umn.edu
Personal Website: http://umn.edu/~frah0010

Please note: Sometimes the University's spam filters are over-
protective and reject wanted messages, especially from free or
overseas accounts. If your message is rejected or you are concerned
that you did not receive a reply from me, please feel free to try my
alternate email account: elleryfrahm-at-mac.com


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From: nmedvitz-at-nephrocor.com
Date: Thu, 14 Jun 2007 19:18:49 -0500
Subject: [Microscopy] viaWWW: Advantages of having two TEMs in the same location rather

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Email: nmedvitz-at-nephrocor.com
Name: Neil Medvitz

Organization: Bostwick Laboratories

Title-Subject: [Filtered] Advantages of having two TEMs in the same location rather than having one EM in two locations

Question: I am writing justification for having two TEMs in the same location rather one EM in two locations. I have a lot of reasons but just want to make sure I am not overlooking anything. Could I please get some opinions?

Thanks to all who help,
Neil

Neil Medvitz
Electron Microscopy Technical Director

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From: drk-at-SHCC.org
Date: Thu, 14 Jun 2007 19:59:23 -0500
Subject: [Microscopy] UPS for TEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings, Fellow Microscopists:

We are renovating our lab to install an FEI Tecnai G2 20TEM. As we think
about the electrical needs, we wonder about the necessity of backing the
system with an uninterrupted power supply and generator. My understanding
is that in the event of a power outage, the system will shut down, the
pneumatic valves will close and the microscope will survive. But I wonder
about the support computers. I am asking for the advice of others in how
they have addressed UPS for their modern TEM systems.

Many thanks,

Doug

Douglas R. Keene
Assistant Investigator
Micro-Imaging Center
Shriners Hospital for Children
3102 S.W. Sam Jackson Park Road
Portland, Oregon 97239
503-221-3434
drk-at-shcc.org



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From: gary-at-gaugler.com
Date: Fri, 15 Jun 2007 00:08:06 -0500
Subject: [Microscopy] Re: UPS for TEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If power goes off, your gun goes off. Of course,
if it is the same as an FESEM.

My solution is two 8KVA dual redundant Liebert
nFinity dual conversion UPS units. Split the
load between the two units. The SEM is on one
unit while the EDS, PCs, chiller and other stuff
are on the other unit. With redundant converters
and controllers, an 8KVA unit does 4KVA with half
of a unit. If one portion fails, the other half works.
Fix the failed unit and back to 100% redundant.

Bad power is a big problem here in CA. Dual conversion
is a huge benefit of these UPS. Steady, constant and
always there. With full set of batteries, I have
about 280 minutes of UPS backup for each UPS. each
unit sees about 22% load.

Full complement of batteries (no expansion unit), and redundant
controllers runs about $10K per unit. They are rock
solid. For about $300 each, you can hook them to a LAN
and monitor what is going on with power. You will be
surprised. Or, manually view the event log.

Liebert makes higher capacity units but these work for my
application.

gary g.


At 05:00 PM 6/14/2007, you wrote:




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From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Fri, 15 Jun 2007 02:33:11 -0500
Subject: [Microscopy] Re: Carbon "contamination"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Gary and all listers

I agrees with the different comments about Pd Mz line overlapping on
Carbon K. I had the same stuff with new polished and ion etched Pt
standards, where I found that intrusive carbon... which was Pd-N line !
And my software don't have the N lines tabulated ! I had too the
probleme with the Pd-M in Pd/Fe alloys.

Added to the overlapping probleme, in some situation one have catalysis
effect on carbon cracking. The carbon peak comes really from carbon, but
groes during the spectrum acquisition ! In such case you should see
these famous hated black squares after the analysis...

About the uncoated sample, a collegue now retired said me never to use
plastic bags or plasctic boxes for sample storage. Most plastics outgas
solvent continusly and polluate all the surfaces with solvent. He used
only glass ware. One need only a monolayer of hydrocarbon to have some
contamination ! People who make Auger spectroscopy know that one must
clean away the carbon before one see something else !

I've made some test, and my conclusion is that in most cases, the sample
and the sample hodler bring with them the major source of contamination.
The second source is the rotary-vane pump, and the last is the diff pump.

The two ways to limit contamination are first to use a lN2 cold trap
very near of the sample, in front of the OL, or beside , or around it
(one must have a fast entry lock), and second to put all parts which
comes to air, the holder and the sample in a plasma cleaner, before
putting them in the SEM (one must have much monney to buy one !). A
simple glow discharge in air can be suffisant, but is not very
reproductible.

About the Supra configuration, just a coment : as we bought our first
FE-SEM, I saw in the test round that the Supra (in that time it was a
1530) was the SEM which had the most contamination from the five SEM we
have seen. It was one raison for an other choice. Now we have bought a
Supra 40 since a few month, for E-beam lithography, a application where
one know that contamination cannot be avoided (one put in samples with a
fresh coating of PMMA or other durty stuffs !), I have seen that, before
performing any litho work, that contamination question was still the
same. People from Leo/Zeiss say that the good performences of their
SE-decteor reveal very thin contamination levels. My thought is that the
vacuum demand isn't high enough. One cannot vent to air inocently a
vaccum chamber with a so big specific surface and a turbo pump at each
sample change without consequences. With the time one catch organic
contamination from the environnement in the SEM itself. This can be one
more source of contamination.

Hope it helps

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



gary-at-gaugler.com a écrit :
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} ----------------------------------------------------------------------------
}
} Greetings all--I am seeking input on what appears
} to be Carbon contamination. Here is the situation.
}
} Take a Pella 16111-9 stub out of bag and put on
} holder. Take another stub out of bag and sputter
} coat with Pd and put on holder. Do EDS on both.
}
} un-coated:
} wt% at%
} C 7.5 15
} O 4.5 7
} Al 88 78
}
} coated:
} C 23 38
} O 6.5 8
} Al 71 53
}
} SEM is Zeiss Supra 55VP with Edwards XDS10 dry
} scroll pump and turbo. Coater is Denton Desk IV
} with Edwards XDS5 and turbo.
}
} The goal of using non-oil pumps was to reduce hydrocarbon
} contamination. So, where is the C coming from? Nothing
} has been done to the scroll pumps since new. There are
} kits for repairing them but when is this necessary and
} what would indicate that it be done? Would high C
} be an indicator?
}
} I'm stumped on this one.
}
} Any ideas?
}
} gary g.
}
}
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From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Fri, 15 Jun 2007 02:40:45 -0500
Subject: [Microscopy] Carbon "contamination" additionnaly a new Quest

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all

In my former mail, in answer to Gary's question, I have said somthing
from the use of the plasma cleaner. I take the opportunity to comme with
a new question.

Did some one still build a plasma cleaner in the SEM itself, or better
in the fast entry lock from the SEM. This would be the most drasctic way
to avoid the contamination coming with the sample/sample holder.

If some has tried, or know from some tests...

Thanks


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



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From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Fri, 15 Jun 2007 07:05:12 -0500
Subject: [Microscopy] Carbon "contamination" oups...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Jaques,
have a look at http://www.semclean.com
It seems to work greatly and is used - to my knowledge - by some major sem
manufacturer - in Europe...

Best regards,
Stefan Diller




----- Original Message -----
X-from: {jacques.faerber-at-ipcms.u-strasbg.fr}
To: {stefan.diller-at-t-online.de}
Sent: Friday, June 15, 2007 9:44 AM

Hi all

Receving just my mail from this morning, I see that I that I have made
an error in the first paragraph. It is the Pt-N line and not the Pd-N
line, which interfer, like the Pd-M.


--
J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr


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From: zaluzec-at-microscopy.com
Date: Fri, 15 Jun 2007 07:59:39 -0500
Subject: [Microscopy] Carbon contamination: Plasma Cleaners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jacque...

To answer your question.

Yes, a plasma cleaner for an EM Chamber is a commerical product.
There have been several papers written on this from both the SEM and TEM standpoint.

You should look at the following WWW site from XEI Scientific for the SEM info. They
have a number of papers on-line to download.

http://www.evactron.com/

Disclaimer:
Argonne National Lab, my daytime employer holds the basic patent on this
technology for EM applications and licenses it to a number of commerical
organizations, XEI being one of them.

Nestor
Your Friendly Neighborhood SysOp.



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Hi all

In my former mail, in answer to Gary's question, I have said somthing
from the use of the plasma cleaner. I take the opportunity to comme with
a new question.

Did some one still build a plasma cleaner in the SEM itself, or better
in the fast entry lock from the SEM. This would be the most drasctic way
to avoid the contamination coming with the sample/sample holder.

If some has tried, or know from some tests...

Thanks


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des MatÈriaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



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From: keith.morris-at-ucl.ac.uk
Date: Fri, 15 Jun 2007 08:19:34 -0500
Subject: [Microscopy] objective NA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi David,

Numerical Aperture: This is a critical value that indicates the light
acceptance angle, which in turn determines the light gathering power, the
resolving power, and depth of field of the objective.

Thus the higher the resolving power (NA) of an objective, the lower the
depth of field (and so working distance). So a high NA objective may not
suite all applications. Indeed a typical high magnification objective focus
point will only just penetrate through a standard 0.17mm glass coverslip. An
air objective will have a far greater (relatively) depth of field and thus a
far longer working distance, particularly an LD 'long working distance'
variety - but the image quality will be noticeably poorer (not a problem
though if you can't see squat with your high NA short working distance oil
immersion lens). This is all to do with airey disks and stuff, [and also a
lot to do with the price of the objective and how well it's made]. Image
contrast, as well as resolution, is also an important consideration here.


'Working distance' correction Collar

An adjustment [correction] collar for cover glass thickness (not NA) is
sometimes provided on high-NA microscope objective lenses. Rotation of the
collar adjusts the height of certain lens elements in the objective lens to
compensate for variations in coverslip thickness or immersion media. Thus it
provides an adjustable working distance. At high NAs, even a small deviation
of the coverslip thickness (by as little as a few micrometers in some
cases), or refractive index of the immersion medium from the designated
standard, can introduce significant aberrations.


Variable NA collar

Other objectives specifically designed for transmitted light fluorescence
and darkfield imaging are sometimes equipped with an internal iris diaphragm
that allows for adjustment of the effective numerical aperture [NA]. A 60x
apochromat objective can have a numerical aperture of 1.4, one of the
highest attainable in modern microscopes using immersion oil as an imaging
medium.

Variable Numerical Aperture Objectives: Specimens with unusually high
fluorescence quantum yields and/or very bright darkfield specimens often
induce image flare by light emitted from areas outside the focal plane. To
compensate for this artefact, manufacturers offer high numerical aperture
objectives that are equipped with an internal iris diaphragm to increase
image contrast during photomicrography or digital imaging. Opening or
closing the iris diaphragm determines the size of the objective rear
aperture yielding a variable numerical aperture range between 0.5 and the
objective's upper limit (up to 1.35-1.4 with apochromatic objectives).
Although iris diaphragms were once utilized in a wide variety of objective
designs, modern variable numerical aperture objectives are usually at the
high end (60x to 150x) of the magnification range.

A variable NA collar on an oil immersion thus help with contrast enhancement
when set to low NA, but it's not going to help it in any way achieve the
long working distance of a dry LD objective of the same magnification. I
must admit that I don't bother too much with the physics equations when
looking down the microscope, as any decent microscope rep should be able
provide you with a set of objectives to assess which one suites your needs
best before you buy. Typically you would go for the highest NA you can for
the given working distance required. Manufacturer's websites provide the
objectives working distance (in mm) and NA info. Modern ultra-high NA
objectives are required for TIRF applications and hi-res DIC contrast
enhancement.

To achieve higher NA than 0.95 for objectives they have to be used with
immersion media between front lens and specimen. Oil or water is mostly used
for that purpose. Because objectives have to be specially designed for this,
the type of immersion media is always mentioned on the objective like on the
UPlanFLN 60x/1.25 Oil Iris objective. This objective needs oil as an
immersion media and can not produce good image quality without it.

High NA objectives also collect more light and if you compare two objectives
with fluorescent samples, the high NA objective should produce a noticeably
brighter (and preferable) image. Again though the high NA objective is
generally far more expensive and it's better attention to quality
optics/coatings will also be factor in it's superior performance. An
objective also needs to be PLAN Apochromat and fluorescence friendly for
quality fluorophore imaging.

The only problem with using our variable NA collar objectives here is that
we use inverted optics and have no idea where the NA collar is actually set
[e.g. if it's moved accidentally or the previous user has adjusted it]. I do
put a chart on the wall telling users which way to turn the NA collar for
highest NA (viewed from above), but I'm not sure all users bother with this.
I have noticed slight changes in um/pixel calibration (less than 10%
difference, and it's linear) between the two NA extremes on some objectives.


To be told more than you probably want to know about resolving power and NA
see:

http://www.olympusmicro.com/primer/anatomy/specialobjectives.html
http://www.microscopy.olympus.eu/microscopes/About_Microscopy_7436.htm
[The resolving power]
http://www.charfac.umn.edu/glossary/c.html
http://www.oznet.ksu.edu/ed_agron645/lab/microscope_use.htm
http://www.olympusconfocal.com/theory/confocalobjectives.html
http://www.microscopyu.com/tutorials/java/objectives/nuaperture/index.html
http://www.microscopyu.com/articles/optics/objectivespecs.html
http://www.micro.magnet.fsu.edu/primer/java/aberrations/slipcorrection/index
.html

Regards

Keith

-----------------------
Dr Keith J Morris
[Formerly] Imaging Facilities Manager
Cell Biology
Institute of Ophthalmology
UCL, London EC1V 9EL




-----Original Message-----
X-from: david.knecht-at-uconn.edu [mailto:david.knecht-at-uconn.edu]
Sent: 14 June 2007 18:44
To: keith.morris-at-ucl.ac.uk

One can buy a 40-100x oil objective with an iris diaphram. What is
the theoretical difference in performance between imaging with a low
NA dry objective (.6NA for example) and an equivalent magnification,
high NA oil objective with the iris partially closed to reduce the NA
to .6? Should they be similar? Thanks- Dave


Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)



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From: paul_hazelton-at-umanitoba.ca
Date: Fri, 15 Jun 2007 08:40:21 -0500
Subject: [Microscopy] UPS for TEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you Gary.

Only saw someone's response to this question last evening and did not
get to respond immediately, but Gary has really hit a major point. It
is more than just protecting against the power outage. Most - if not
all - microscopes have all sorts of emergency backups which go in place
to prevent any real damage. Power outages are not common, butd they are
major irritations when they occur. A real problem is spikes in power -
the dirty power Gary refers to.

When I took over my current position it took me 18 months to figure out
that the separate and isolated power source for my lab was neither
separate nor isolated. The department had even put in (before me) a
nice Barrie anti vibration system - works great, didn't stop the
instability problems. We finally figured out what was happening when it
was noticed that the aberrations in beam and focus occurred whenever
someone was doing gel electrophoresis in a bank of labs in the next
hallway. When we put a monitor on the power in line we found over 130
spikes in power in excess of 5% over the normal 12hour daytime period.

Fluctuations in power - spikes - do occur regularly. Not just in
California, but in Winnipeg, Montreal, London, New York, Tokyo, etc.
Surge protectors kick in after the first cycle of a spike. As a result,
they do not protect against spikes with your computers and other 'not a
microscope' equipment, they . Other than the glowing little light and
giving you 5 plugs from 1 they probably do nothing to help.

Damage occurs in the first spike. This is true of the EM also. Dirty
lines mean pops in and out of focus. If you've ever seen one, you know
what this is. Imagine the fresnel fringe popping in and out
concentrically while you look at the image you are trying to see. It
may not bother you when you look at a section at 10,000, but it will
drive you nuts when looking at a virus at 100,000+.

I didn't put in a UPS at the time. 25 years ago the unit would have
cost } 10,000 and needed it's own room. But when we moved a microscope
recently during a lab consolidation I built into the cost $2500 to put
in a UPS. The best time to get things from administrators is when you
are building a new lab, moving, renovating, or putting in a new
microscope. Even if you have to spend Gary's $10-12,000US to protect
several microscopes, or my $2,200C to protect one microscope, it is
worth it.


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From: dljones-at-bestweb.net
Date: Fri, 15 Jun 2007 09:02:44 -0500
Subject: [Microscopy] Re: Buehler IsoMet

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ellery,

I have the same saw, functioning, and still have the original manual that came
with it. So if you'd like, I can send you a copy.

However, I would suggest that you just call Buehler. I'm sure thay have a .pdf
file with the manual and would send it to you. Buehler has sent me manuals,
calibration data, all kinds of information on older equipment that they
manufactured. They are really top notch in this regard, at least that has been
my experience.

dj

On Thu, 14 Jun 2007, frah0010-at-umn.edu wrote:

}
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} Microscopists,
}
} Our lab has a Buehler IsoMet slow saw (model #11-1180, apparently
} 1980s vintage) that has ceased functioning. I've tracked the problem
} down to broken gear teeth on the shaft perpendicular to the motor's
} drive shaft. Does anyone else have a dead or unused IsoMet that they
} wouldn't mind someone cannibalizing? We can pay shipping and/or
} modestly for the equipment/parts. We also don't have the
} instructions or specifications anymore. If someone else does and
} there are good part descriptions, I'd be interested in a fax or scan
} of that page to help track down a replacement part. Thanks in advance!
}
} Cheers,
} Ellery
}
} --------------------
} Ellery E. Frahm
} Research Fellow & Manager
} Electron Microprobe Laboratory
} University of Minnesota - Twin Cities
} Department of Geology & Geophysics
} Lab Website: http://probelab.geo.umn.edu
} Personal Website: http://umn.edu/~frah0010
}
} Please note: Sometimes the University's spam filters are over-
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} that you did not receive a reply from me, please feel free to try my
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From: david.knecht-at-uconn.edu
Date: Fri, 15 Jun 2007 09:20:25 -0500
Subject: [Microscopy] objective NA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Lots of useful information in the responses, but let me clarify the
question. A colleague is using his stopped down 40x oil objective in
order to visualize vesicles throughout the volume of the cell in one
image rather than confocal sectioning. I am trying to understand why
it works and whether it would work equivalently with a dry
objective. Obviously the light cone from a 1.4 NA 40x oil objective
(whether iris is closed or not) and a 40x long working distance dry
objective are different. The depth of field varies with numerical
aperture, so a 40x 1.4 oil immersion lens, should inherently have a
shallower depth of focus. Therefore, it makes no intuitive sense to
me that you would #1-increase the depth of focus, and #2- get the
same results with a 40x oil 1.4 in which you stop down the iris to .
5NA, as a .5NA LWD dry lens. The excitation spot profile of the 40x
oil objective is not going to enlarge as you stop down the iris with
the same input light path. So I am trying to understand how the
focal depth/resolution/xyz profile of the excitation/emission etc. is
for fluorescence in comparing those two situations. Dave

Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)


On Jun 15, 2007, at 9:19 AM, keith.morris-at-ucl.ac.uk wrote:

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} America
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} MicroscopyListserver
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} ----------------------------------------------------------------------
} ------
}
} Hi David,
}
} Numerical Aperture: This is a critical value that indicates the light
} acceptance angle, which in turn determines the light gathering
} power, the
} resolving power, and depth of field of the objective.
}
} Thus the higher the resolving power (NA) of an objective, the lower
} the
} depth of field (and so working distance). So a high NA objective
} may not
} suite all applications. Indeed a typical high magnification
} objective focus
} point will only just penetrate through a standard 0.17mm glass
} coverslip. An
} air objective will have a far greater (relatively) depth of field
} and thus a
} far longer working distance, particularly an LD 'long working
} distance'
} variety - but the image quality will be noticeably poorer (not a
} problem
} though if you can't see squat with your high NA short working
} distance oil
} immersion lens). This is all to do with airey disks and stuff, [and
} also a
} lot to do with the price of the objective and how well it's made].
} Image
} contrast, as well as resolution, is also an important consideration
} here.
}
}
} 'Working distance' correction Collar
}
} An adjustment [correction] collar for cover glass thickness (not
} NA) is
} sometimes provided on high-NA microscope objective lenses. Rotation
} of the
} collar adjusts the height of certain lens elements in the objective
} lens to
} compensate for variations in coverslip thickness or immersion
} media. Thus it
} provides an adjustable working distance. At high NAs, even a small
} deviation
} of the coverslip thickness (by as little as a few micrometers in some
} cases), or refractive index of the immersion medium from the
} designated
} standard, can introduce significant aberrations.
}
}
} Variable NA collar
}
} Other objectives specifically designed for transmitted light
} fluorescence
} and darkfield imaging are sometimes equipped with an internal iris
} diaphragm
} that allows for adjustment of the effective numerical aperture
} [NA]. A 60x
} apochromat objective can have a numerical aperture of 1.4, one of the
} highest attainable in modern microscopes using immersion oil as an
} imaging
} medium.
}
} Variable Numerical Aperture Objectives: Specimens with unusually high
} fluorescence quantum yields and/or very bright darkfield specimens
} often
} induce image flare by light emitted from areas outside the focal
} plane. To
} compensate for this artefact, manufacturers offer high numerical
} aperture
} objectives that are equipped with an internal iris diaphragm to
} increase
} image contrast during photomicrography or digital imaging. Opening or
} closing the iris diaphragm determines the size of the objective rear
} aperture yielding a variable numerical aperture range between 0.5
} and the
} objective's upper limit (up to 1.35-1.4 with apochromatic objectives).
} Although iris diaphragms were once utilized in a wide variety of
} objective
} designs, modern variable numerical aperture objectives are usually
} at the
} high end (60x to 150x) of the magnification range.
}
} A variable NA collar on an oil immersion thus help with contrast
} enhancement
} when set to low NA, but it's not going to help it in any way
} achieve the
} long working distance of a dry LD objective of the same
} magnification. I
} must admit that I don't bother too much with the physics equations
} when
} looking down the microscope, as any decent microscope rep should be
} able
} provide you with a set of objectives to assess which one suites
} your needs
} best before you buy. Typically you would go for the highest NA you
} can for
} the given working distance required. Manufacturer's websites
} provide the
} objectives working distance (in mm) and NA info. Modern ultra-high NA
} objectives are required for TIRF applications and hi-res DIC contrast
} enhancement.
}
} To achieve higher NA than 0.95 for objectives they have to be used
} with
} immersion media between front lens and specimen. Oil or water is
} mostly used
} for that purpose. Because objectives have to be specially designed
} for this,
} the type of immersion media is always mentioned on the objective
} like on the
} UPlanFLN 60x/1.25 Oil Iris objective. This objective needs oil as an
} immersion media and can not produce good image quality without it.
}
} High NA objectives also collect more light and if you compare two
} objectives
} with fluorescent samples, the high NA objective should produce a
} noticeably
} brighter (and preferable) image. Again though the high NA objective is
} generally far more expensive and it's better attention to quality
} optics/coatings will also be factor in it's superior performance. An
} objective also needs to be PLAN Apochromat and fluorescence
} friendly for
} quality fluorophore imaging.
}
} The only problem with using our variable NA collar objectives here
} is that
} we use inverted optics and have no idea where the NA collar is
} actually set
} [e.g. if it's moved accidentally or the previous user has adjusted
} it]. I do
} put a chart on the wall telling users which way to turn the NA
} collar for
} highest NA (viewed from above), but I'm not sure all users bother
} with this.
} I have noticed slight changes in um/pixel calibration (less than 10%
} difference, and it's linear) between the two NA extremes on some
} objectives.
}
}
} To be told more than you probably want to know about resolving
} power and NA
} see:
}
} http://www.olympusmicro.com/primer/anatomy/specialobjectives.html
} http://www.microscopy.olympus.eu/microscopes/About_Microscopy_7436.htm
} [The resolving power]
} http://www.charfac.umn.edu/glossary/c.html
} http://www.oznet.ksu.edu/ed_agron645/lab/microscope_use.htm
} http://www.olympusconfocal.com/theory/confocalobjectives.html
} http://www.microscopyu.com/tutorials/java/objectives/nuaperture/
} index.html
} http://www.microscopyu.com/articles/optics/objectivespecs.html
} http://www.micro.magnet.fsu.edu/primer/java/aberrations/
} slipcorrection/index
} .html
}
} Regards
}
} Keith
}
} -----------------------
} Dr Keith J Morris
} [Formerly] Imaging Facilities Manager
} Cell Biology
} Institute of Ophthalmology
} UCL, London EC1V 9EL
}
}
}
}
} -----Original Message-----
} X-from: david.knecht-at-uconn.edu [mailto:david.knecht-at-uconn.edu]
} Sent: 14 June 2007 18:44
} To: keith.morris-at-ucl.ac.uk
} Subject: [Microscopy] objective NA
}
}
}
}
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}
} One can buy a 40-100x oil objective with an iris diaphram. What is
} the theoretical difference in performance between imaging with a low
} NA dry objective (.6NA for example) and an equivalent magnification,
} high NA oil objective with the iris partially closed to reduce the NA
} to .6? Should they be similar? Thanks- Dave
}
}
} Dr. David Knecht
} Department of Molecular and Cell Biology
} Co-head Flow Cytometry and Confocal Microscopy Facility
} U-3125
} 91 N. Eagleville Rd.
} University of Connecticut
} Storrs, CT 06269
} 860-486-2200
} 860-486-4331 (fax)
}
}
}
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} Headers==============================
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From: ph2-at-sprynet.com
Date: Fri, 15 Jun 2007 10:42:18 -0500
Subject: [Microscopy] Inter/Micro 2007

Contents Retrieved from Microscopy Listserver Archives
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I am forwarding an Extracted/Shortened section:


Inter/Micro 2007 Announcements - June 8th, 2007

See: http://www.mcri.org/IM_info_page.html

The Inter/Micro 2007 conference, scheduled for July 9-13, 2007, is rapidly
approaching!

Announcing the symposium's schedule for speakers:

2007 Schedule of Presentations

See http://www.mcri.org/2007ScheduleforSpeakers.pdf

Thursday Workshop:

'Working with Living Cells - Triumphs, Tribulations and Tragedies', Jeremy
Pickett-Heaps of the School of Botany, University of Melbourne, & Brian J.
Ford of Gonville & Caius College, Cambridge University.

Friday Workshop: 'Airborne & Settled Dust Particle Identification
Workshop', Andrew A. Havics, pH2 LLC., Randy Boltin, MVA Scientific
Consultants, & John D. Shane, Ph.D., PRO-LAB/SSPTM, Inc.



Tony

Ps Disclaimer - I receive no profits from this although I'm teaching part
of the one workshop on Friday.

......................................................................
Andrew Anthony "Tony" Havics, CHMM, CIH, PE
pH2, LLC
5250 E US 36, Suite 830
Avon, IN 46123
(317) 718-7020 off
(317) 718-7038 fax
(317) 409-3238 cell

90% of Risk Management is knowing where to place the decimal point...any
consultant can give you the other 10%(SM)

This message is from pH2. This message and any attachments may contain
legally privileged or confidential information, and are intended only for
the individual or entity identified above as the addressee. If you are not
the addressee, or if this message has been addressed to you in error, you
are not authorized to read, copy, or distribute this message and any
attachments, and we ask that you please delete this message and attachments
(including all copies) and notify the sender by return e-mail or by phone at
317-718-7020. Delivery of this message and any attachments to any person
other than the intended recipient(s) is not intended in any way to waive
confidentiality or a privilege. All personal messages express views only of
the sender, which are not to be attributed to pH2 and may not be copied or
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From: gary-at-gaugler.com
Date: Fri, 15 Jun 2007 11:20:12 -0500
Subject: [Microscopy] Re: UPS for TEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Yes... I should have amplified about the UPS units.

Disregarding outages (that's what the UPS are for),
spikes and sags are bad and can have catastrophic
effects on the equipment (SEM, TEM, PCs, etc.).

There are basically two types of UPS. One is a simple
APC brand style that switches on an inverter when the
input power fails. These units can include spike clamps
but do not handle sags. The second type is double conversion.
This is the best method. This type takes input AC and
converts it to DC (usually around 240VDC) and inverts
it to AC via sine wave driver and LARGE output transformer.
This produces pure sine wave output voltage with about 3%
stability and total protection from sags and spikes.

If the input power fails, the unit instantly switches from
the rectified input AC--} DC to the battery bank. Perfect.
Plus, the Liebert has redundancy options. In my Amray
days, I used a pair of Toshiba 1400xl+ dual conversion
UPS. They worked well but did not have redundancy. The
problem with this is that when one failed, the whole system
went down. US service support for the Toshiba units was bad.
The Liebert units are US made and US supported. After almost
two years of continuous operation, neither of the Lieberts
has failed. For about $1K, extended warranty is available.
This covers the electronics and the batteries. Each battery
is actually a module itself and if the monitors think a
battery is bad, a light comes on on the battery module and
a message is posted about it.

Getting a good UPS at install is the way to go. For a
fraction of the cost of the SEM or TEM and accessories,
it is cheap insurance.

gary g.


At 05:41 AM 6/15/2007, you wrote:




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From: tivol-at-caltech.edu
Date: Fri, 15 Jun 2007 12:21:03 -0500
Subject: [Microscopy] Re: UPS for TEMs

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On Jun 14, 2007, at 5:59 PM, drk-at-SHCC.org wrote:

} We are renovating our lab to install an FEI Tecnai G2 20TEM. As we
} think
} about the electrical needs, we wonder about the necessity of backing
} the
} system with an uninterrupted power supply and generator. My
} understanding
} is that in the event of a power outage, the system will shut down, the
} pneumatic valves will close and the microscope will survive. But I
} wonder
} about the support computers. I am asking for the advice of others in
} how
} they have addressed UPS for their modern TEM systems.
}
Dear Doug,
We have a T12 and a TF30H, and we only have a UPS on the 30. We have
not had any power failures that affected the computers, but we did have
a failure in the UPS (fortunately, no power failures during the several
months it took to get the UPS repair booked and done).
Yours,
Bill Tivol, PhD
EM Scientist
Electron Cryo-Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


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From: rich_goodheart-at-agilent.com
Date: Fri, 15 Jun 2007 17:37:37 -0500
Subject: [Microscopy] viaWWW: Seminar on SPM, AFM, STM In Undergraduate Education

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Email: rich_goodheart-at-agilent.com
Name: Rich Goodheart

Organization: Agilent Technologies

Title-Subject: [Filtered] SPM, AFM, STM In Undergraduate Education

Question: On June 26, 2007, Agilent Technologies will be hosting a free web-based seminar on the Integration of AFM in Undergraduate Education. Jayne Garno, an assistant professor of chemistry at Louisiana State University, is working to integrate scanning probe experiments into junior and senior undergraduate laboratory courses in analytical and physical chemistry to give students hands-on experience with molecular imaging. In this informative eSeminar, she will describe her progress. Following Garno's presentation, Dr. Song Xu, an applications scientist with Agilent Technologies, will discuss research-grade AFM instrumentation appropriate for use in undergraduate education. There will also be a Q&A session in which all online attendees are welcome to query the presenters. More information, and to register, can be found at http://nano.tm.agilent.com

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From: keith.morris-at-ucl.ac.uk
Date: Sat, 16 Jun 2007 08:19:51 -0500
Subject: [Microscopy] objective NA

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Hi David,

I didn't get a copy of my reply in the in-box (you obviously did), so it's
also attached to the bottom here. Essentially the NA iris works like the
iris on the condenser and a camera (to the eye, I don't know about the
optical physics).

Using a camera with a macro lens, stopping [closing] the camera iris down to
F22 brings everything in the macro shot into focus (a big depth of field),
but at the expense of image brightness and probably a little resolution - so
you need a longer exposure time. You aren't actually moving the camera any
closer to the specimen though, so the 'working distance' hasn't changed.
Plus your point of focus is still in the same place (as the lenses/camera
haven't moved). See
http://www.cs.mtu.edu/~shene/DigiCam/User-Guide/950/depth-of-field.html.

Stopping the NA iris in the high mag oil objective down (to low NA)
increases the contrast and probably the depth of field around the focus
point, but no doubt at the expense of absolute resolution (hence the lower
NA). Naturally high resolution is pig all use without any contrast though to
actually see the specimen, hence the advantage of the iris. It probably
won't affect the maximum working distance at all though (you are still under
oil), other than perhaps a greater depth of focus around the area you are
focussed on (you can't physically move the objective into the sample any
more). To increase actual working distance you have move the internal lenses
(or one anyway) up and down, using a Working Distance [WD] collar if fitted
(I've never seen a variable NA iris and variable WD lens collar fitted
together on one objective though).

The increased contrast and greater depth of field at the lower NA iris
setting will bring out intra-cellular structures far better, and it works
with DIC as well. We use a Leica 63x [1.4-0.6 variable NA] HCX PL APO oil
objective, which cost around £5,000 I believe. Partly due to it being a
flash well made expensive objective, this Leica 'blue' variable NA 63x oil
objective's image quality will always be far better than that obtained our
long working distance Olympus LCPLFL 60x air objective [NA 0.7], the image
quality of which is relatively appalling - but of course it can see well
into the gels we use, unlike the Leica high NA oil version, and so is our
only choice for such things. The Olympus 60x also has a variable NA to
adjust the focus depth and contrast around the point of focus, but image
quality at either NA extreme is still quite poor compared to the Leica oil
(but it has that long working distance and can be physically moved to focus
far further into the specimen).

Well that’s how I see it anyway (and it must be true as another guy at a
conference I was at said a similar thing to me once). Unfortunately I don't
have access to these objectives any more, as I would like to investigate it
further (that’s the fun of this list-server it generates interesting
questions).

We generally use these variable NA 63x objectives with fluorescent samples
and z-slices under a laser confocal, so I doubt we'd notice any depth of
field improvements with the lower NA setting as we are sticking the centre
of the focus anyway.

Regards

Keith

-----------------------
Dr Keith J Morris
[Formerly] Imaging Facilities Manager
Cell Biology
Institute of Ophthalmology
UCL, London EC1V 9EL

----------------------------------------------------------------------------
-





Hi David,

Numerical Aperture: This is a critical value that indicates the light
acceptance angle, which in turn determines the light gathering power, the
resolving power, and depth of field of the objective.

Thus the higher the resolving power (NA) of an objective, the lower the
depth of field (and so working distance). So a high NA objective may not
suite all applications. Indeed a typical high magnification objective focus
point will only just penetrate through a standard 0.17mm glass coverslip. An
air objective will have a far greater (relatively) depth of field and thus a
far longer working distance, particularly an LD 'long working distance'
variety - but the image quality will be noticeably poorer (not a problem
though if you can't see squat with your high NA short working distance oil
immersion lens). This is all to do with airey disks and stuff, [and also a
lot to do with the price of the objective and how well it's made]. Image
contrast, as well as resolution, is also an important consideration here.


'Working distance' correction Collar

An adjustment [correction] collar for cover glass thickness (not NA) is
sometimes provided on high-NA microscope objective lenses. Rotation of the
collar adjusts the height of certain lens elements in the objective lens to
compensate for variations in coverslip thickness or immersion media. Thus it
provides an adjustable working distance. At high NAs, even a small deviation
of the coverslip thickness (by as little as a few micrometers in some
cases), or refractive index of the immersion medium from the designated
standard, can introduce significant aberrations.


Variable NA collar

Other objectives specifically designed for transmitted light fluorescence
and darkfield imaging are sometimes equipped with an internal iris diaphragm
that allows for adjustment of the effective numerical aperture [NA]. A 60x
apochromat objective can have a numerical aperture of 1.4, one of the
highest attainable in modern microscopes using immersion oil as an imaging
medium.

Variable Numerical Aperture Objectives: Specimens with unusually high
fluorescence quantum yields and/or very bright darkfield specimens often
induce image flare by light emitted from areas outside the focal plane. To
compensate for this artefact, manufacturers offer high numerical aperture
objectives that are equipped with an internal iris diaphragm to increase
image contrast during photomicrography or digital imaging. Opening or
closing the iris diaphragm determines the size of the objective rear
aperture yielding a variable numerical aperture range between 0.5 and the
objective's upper limit (up to 1.35-1.4 with apochromatic objectives).
Although iris diaphragms were once utilized in a wide variety of objective
designs, modern variable numerical aperture objectives are usually at the
high end (60x to 150x) of the magnification range.

A variable NA collar on an oil immersion thus help with contrast enhancement
when set to low NA, but it's not going to help it in any way achieve the
long working distance of a dry LD objective of the same magnification. I
must admit that I don't bother too much with the physics equations when
looking down the microscope, as any decent microscope rep should be able
provide you with a set of objectives to assess which one suites your needs
best before you buy. Typically you would go for the highest NA you can for
the given working distance required. Manufacturer's websites provide the
objectives working distance (in mm) and NA info. Modern ultra-high NA
objectives are required for TIRF applications and hi-res DIC contrast
enhancement.

To achieve higher NA than 0.95 for objectives they have to be used with
immersion media between front lens and specimen. Oil or water is mostly used
for that purpose. Because objectives have to be specially designed for this,
the type of immersion media is always mentioned on the objective like on the
UPlanFLN 60x/1.25 Oil Iris objective. This objective needs oil as an
immersion media and can not produce good image quality without it.

High NA objectives also collect more light and if you compare two objectives
with fluorescent samples, the high NA objective should produce a noticeably
brighter (and preferable) image. Again though the high NA objective is
generally far more expensive and it's better attention to quality
optics/coatings will also be factor in it's superior performance. An
objective also needs to be PLAN Apochromat and fluorescence friendly for
quality fluorophore imaging.

The only problem with using our variable NA collar objectives here is that
we use inverted optics and have no idea where the NA collar is actually set
[e.g. if it's moved accidentally or the previous user has adjusted it]. I do
put a chart on the wall telling users which way to turn the NA collar for
highest NA (viewed from above), but I'm not sure all users bother with this.
I have noticed slight changes in um/pixel calibration (less than 10%
difference, and it's linear) between the two NA extremes on some objectives.


To be told more than you probably want to know about resolving power and NA
see:

http://www.olympusmicro.com/primer/anatomy/specialobjectives.html
http://www.microscopy.olympus.eu/microscopes/About_Microscopy_7436.htm
[The resolving power]
http://www.charfac.umn.edu/glossary/c.html
http://www.oznet.ksu.edu/ed_agron645/lab/microscope_use.htm
http://www.olympusconfocal.com/theory/confocalobjectives.html
http://www.microscopyu.com/tutorials/java/objectives/nuaperture/index.html
http://www.microscopyu.com/articles/optics/objectivespecs.html
http://www.micro.magnet.fsu.edu/primer/java/aberrations/slipcorrection/index
.html

Regards

Keith

-----------------------
Dr Keith J Morris
[Formerly] Imaging Facilities Manager
Cell Biology
Institute of Ophthalmology
UCL, London EC1V 9EL


-----Original Message-----
X-from: david.knecht-at-uconn.edu [mailto:david.knecht-at-uconn.edu]
Sent: 15 June 2007 15:25
To: keith.morris-at-ucl.ac.uk

Lots of useful information in the responses, but let me clarify the
question. A colleague is using his stopped down 40x oil objective in
order to visualize vesicles throughout the volume of the cell in one
image rather than confocal sectioning. I am trying to understand why
it works and whether it would work equivalently with a dry
objective. Obviously the light cone from a 1.4 NA 40x oil objective
(whether iris is closed or not) and a 40x long working distance dry
objective are different. The depth of field varies with numerical
aperture, so a 40x 1.4 oil immersion lens, should inherently have a
shallower depth of focus. Therefore, it makes no intuitive sense to
me that you would #1-increase the depth of focus, and #2- get the
same results with a 40x oil 1.4 in which you stop down the iris to .
5NA, as a .5NA LWD dry lens. The excitation spot profile of the 40x
oil objective is not going to enlarge as you stop down the iris with
the same input light path. So I am trying to understand how the
focal depth/resolution/xyz profile of the excitation/emission etc. is
for fluorescence in comparing those two situations. Dave

Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)


On Jun 15, 2007, at 9:19 AM, keith.morris-at-ucl.ac.uk wrote:




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From: dljones-at-bestweb.net
Date: Mon, 18 Jun 2007 14:17:33 -0500
Subject: [Microscopy] unusual request

Contents Retrieved from Microscopy Listserver Archives
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Hello all,

OK, I've been looking around for a group that talks/works on/etc. older
microscopes. But I have not been successful in finding such a group.

I have just picked up an old Olympus PMG metallograph. It is an absolutely
fascinating piece of optical equipment. I've never seen one like it.
Anyway, as one of my (ever increasing number of) extra-curricula
activities, I've decided I'd like to either restore this instrument, or
alter it so that I can actually use it as a modern metallograph with
digital imaging capabilities. The first option would be my first choice,
but if I can't get the parts needed to do that, I'll shoot for the second
option...

My question to all the extraordinary minds on this group, does anyone know
of someone that works on old microscopes? Does anyone know of a society or
a group that discusses them, has old technical information about them,
things like that?

I apologize if this is way off topic for this group. Please respond
directly to me as I doubt the responses would be of general interest. If
anyone does wish for a list of what I find, I'll be happy to share it,
just let me know.

dj


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From: tjzhang-at-umd.edu
Date: Mon, 18 Jun 2007 15:29:11 -0500
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: wanted ESEM E3

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Email: tjzhang-at-umd.edu
Name: Tim Zhang

Organization: UMD

Title-Subject: [Filtered] " wanted

Question: Dear All,

I need whole set of box of HV /Filament current Generator of "ESEM E3" urgently. I would like to "trade in" with any part of "ESEM E3" in my lab such as sample stage,HV cable, mult-DC power supply and so on.

If you have the circuit diagram of HV/Filament current Generator it would be great helpful.

Thanks for your time.

Tim



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From: HStahlberg-at-ucdavis.edu
Date: Mon, 18 Jun 2007 16:42:32 -0500
Subject: [Microscopy] Cryo-EM postdoc or senior position at UC Davis, California

Contents Retrieved from Microscopy Listserver Archives
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Hi,

We are looking for a postdoctoral or senior (S)TEM expert to join our
group. A background in Materials Sciences, Physics, Engineering or
Bio/Life Sciences would all be suitable.
Projects concern the high-resolution structure determination of
various membrane proteins and of DNA binding proteins, by electron
crystallography and single particle cryo-EM, as well as method
development in electron microscopy hardware for (S)TEM and remote
controlling.
Instrumentation is shared between our and the neighboring laboratory
of Holland Cheng, and includes two or three FEG and one LaB6 TEMs,
featuring cryo (including Helium) capabilities, energy filtration,
STEM, Cs aberration correction, and large CCDs.
Long-term funding is available. Salary will be commensurate with
experience.
Davis, California, is a family-friendly University town, located
between the San Francisco / Berkeley bay area, and the Tahoe mountain
lake and skiing area.

For further info, please contact me by email or phone, or at the
upcoming 3DEM GRC.

Henning.

Henning Stahlberg, PhD
Associate Professor,
Molecular & Cellular Biology, Briggs Hall 5,
University of California at Davis, 1 Shields Ave., Davis, CA 95616, USA
Tel: +1-530-752 8282 (office), +1-530-754 8285 (lab) Fax:
+1-530-752 3085
mailto:HStahlberg-at-ucdavis.edu Skype:henningstahlberg
http://stahlberglab.ucdavis.edu
http://2dx.org
_____________________________________________________________




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From: tina-at-pbrc.hawaii.edu
Date: Mon, 18 Jun 2007 23:40:53 -0500
Subject: [Microscopy] Fixing low pH extremophiles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Once upon a time I had to perform TEM on pineapples of different stages of
ripeness, and I had problems that someone said was because glutaraldehyde
doesn't fix well at low pH. (Nevermind the more pressing problem was the
crystal of Si in each cell, and all that pineapple juice...) Now I have
clients who want to fix bacteria that are being cultured at a pH of about
3.0. Do any of you have any suggestions for a fixation protocol? Cryo is
not an option at this time.

Mahalo,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


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From: lhermini-at-epoisses.inra.fr
Date: Tue, 19 Jun 2007 07:38:37 -0500
Subject: [Microscopy] viaWWW: colloidal gold conjugation of proteins

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Email: lhermini-at-epoisses.inra.fr
Name: jeannine Lherminier

Organization: INRA

Title-Subject: [Filtered] colloidal gold conjugation of proteins

Question: I am interested to preprare a protein-gold (5nm) complex to follow internalization of the protein into the cell. I used the protocols described by Hayat (colloidal gold , Principles, Methods and Applications) and previous recommandations of M. Bendayan but I failed... The protein is a small protein of 10kDa with a pHi of 8,5 and is dissolved in water. In parallel, I performed a control BSA-colloidal gold complex and it is OK. I tried to work with a pH of the gold solution slighly basic to the isoelectric pH of the protein, pH=9. I also tried to work at a pH of 7,4 and I also failed. In both cases, I added the colloidal gold (pH=9 or pH=7.4) to the protein solution. As recommended by M. Bendayan, I did not use at this step any PEG or other protective agents. I then centriguged the mixture at 45,000 rpm at 4ƒC for 60 min. The sediment at the bottom is dark-red. I recovered the sediment in PBS containing 0.02% PEG 20000. When I checked with negative staining with TEM, I did not visualise any gold particles or very few!!!.
Is it the molecular weight of the protein a problem? Is the pH of the gold solution not optimal? Is a protective agent has to be used to stabilize the mixture befor centrifugation?
Thanks a lot

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From: skperkin-at-vt.edu
Date: Tue, 19 Jun 2007 09:50:00 -0500
Subject: [Microscopy] tissue storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello-

Does anyone have experience storing glutaraldehyde-fixed tissues for potential
TEM in 0.1M cacodylate buffer, 5% sucrose and thiomersal? Tissues may be
stored for up to 3 months.

Thanks in advance!
Sandy Hancock


VMRCVM
Virginia Tech

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From: edelmare-at-muohio.edu
Date: Tue, 19 Jun 2007 11:09:29 -0500
Subject: [Microscopy] 200KeV TEM users Opinions, please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

200Kev scope users:

O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with
LaB6 guns. Our biggest concerns come in routine usability, and I am
hoping to hear any comments on any of the following questions we
have.

We handle a wide range of samples (Biological thin sections, organic
macro-molecules, geologic particles, geologic thin sections,
crystals, thin films, nano-particles, organic and non-organic
materials) with a wide range of techniques (BF, DF, Diffraction, EDS,
EELS, including lattice fringe imaging, STEM and tomography
secondarily). In balancing contrast, resolution, and beam damage we
really expect to perform a majority of our imaging between 80KeV and
120KeV (or 150KeV). However, the option of higher voltages up to
200KeV, for higher resolution imaging is something we are
considering. So we are looking at both 120 and 200 KeV scopes. (And
yes, we will test as many samples on any scope as we can but a couple
of days worth of playing with a handful of samples does not compare
to years worth of experience.)

(1) It seems that most 200Kev scopes are set to 200Kev and left
there - presumably because it eases alignments, voltage stability,
etc. when pushing for ultimate resolution. Is this true? Yes, all
the manufacturers list voltage ranges the scope can be operated at,
but does anyone really use anything else? With the digitizing of the
scope controls and storage of multiple alignment setting, alignment
at different voltages (theoretically) should be fairly straight
forward but is it really?

(2) Is 200KeV worth having if the majority of the work does not
require 200KeV? Not having personally worked much at 200KeV, Does
loss of contrast and beam damage at 200KeV limit usability when
imaging other than atomic / lattice level?

(3) If the majority of the scope usage is 80 - 140 KeV is the cost
of a 200KeV instrument worth it? In terms of initial cost, and
maintenance. (Even if the only option tops off at 120KeV). We are
all aware of instances of: "If you can at all afford it get, even if
you won´t use it regularly, because . . ." is this one of those?

(4) A few years ago the list had a discussion of W vs. LaB6, vs.
FEG/Schottky for SEM´s and the consensus basically was that LaB6 was
not worth the cost for SEM. (Either FEG if affordable or tungsten
with better bells & whistles). Is LaB6 worth it in terms of TEM?

(5) What more important issue have we neglected in our naivete?

(Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?)

Thank you for any info you're willing to share!



Richard E. Edelmann, Ph.D.
EXPO Editor, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu



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From: maryflet-at-interchange.ubc.ca
Date: Tue, 19 Jun 2007 11:49:30 -0500
Subject: [Microscopy] 200KeV TEM users Opinions, please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Richard,
The first misconception is that 200 kV causes MORE beam damage than 100 kV.
It causes less. More beam goes through the sample without dumping its
energy, therefore less heat is dumped into the sample and there is less beam
damage. Usable viewing area and diffraction is much improved at 200 kV. I
have a 200 kV W-filament TEM. I use it at mainly 100 kV and 200 kV: 100 kV
for light-element samples to get the contrast and 200 kV for better
resolution and better penetration, therefore larger viewing area in metal
samples. I have also looked at thick, unstained sections in botanical
samples (0.5 micron, 0.75 micron and 1.0 micron thick) at 200 kV. If I were
to replace this very old TEM, it would be with a 200 kV or 300 kV LaB6. The
extra brightness is worth the small extra cost, the FEG in TEM is very
expensive. I would get the STEM option so I could do EDS and EDS mapping.
My TEM has five steps of acceleration voltage: 75, 100, 125, 150, 175 and
200 kV. All can be independently aligned and the alignment stored. Then,
changing kV is simply pushing the button. In the new scopes, you can store
the alignment on a CD or hard drive, so you can recover if one of your users
screws things up horribly.
I envy you the chance, I cannot get the people here to go seriously after a
new TEM. Good luck.
Regards,

Mary Fletcher (nee Mager)
Electron Microscopist
Materials Eng. UBC
#309 - 6350 Stores Road
Vancouver, B.C. V6T 1Z4
Canada
Tel: 604-822-5648
Fax: 604-822-3619
email: maryflet-at-interchange.ubc.ca


-----Original Message-----
X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu]
Sent: June 19, 2007 9:18 AM
To: maryflet-at-interchange.ubc.ca

200Kev scope users:

O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with
LaB6 guns. Our biggest concerns come in routine usability, and I am
hoping to hear any comments on any of the following questions we
have.

We handle a wide range of samples (Biological thin sections, organic
macro-molecules, geologic particles, geologic thin sections,
crystals, thin films, nano-particles, organic and non-organic
materials) with a wide range of techniques (BF, DF, Diffraction, EDS,
EELS, including lattice fringe imaging, STEM and tomography
secondarily). In balancing contrast, resolution, and beam damage we
really expect to perform a majority of our imaging between 80KeV and
120KeV (or 150KeV). However, the option of higher voltages up to
200KeV, for higher resolution imaging is something we are
considering. So we are looking at both 120 and 200 KeV scopes. (And
yes, we will test as many samples on any scope as we can but a couple
of days worth of playing with a handful of samples does not compare
to years worth of experience.)

(1) It seems that most 200Kev scopes are set to 200Kev and left
there - presumably because it eases alignments, voltage stability,
etc. when pushing for ultimate resolution. Is this true? Yes, all
the manufacturers list voltage ranges the scope can be operated at,
but does anyone really use anything else? With the digitizing of the
scope controls and storage of multiple alignment setting, alignment
at different voltages (theoretically) should be fairly straight
forward but is it really?

(2) Is 200KeV worth having if the majority of the work does not
require 200KeV? Not having personally worked much at 200KeV, Does
loss of contrast and beam damage at 200KeV limit usability when
imaging other than atomic / lattice level?

(3) If the majority of the scope usage is 80 - 140 KeV is the cost
of a 200KeV instrument worth it? In terms of initial cost, and
maintenance. (Even if the only option tops off at 120KeV). We are
all aware of instances of: "If you can at all afford it get, even if
you won´t use it regularly, because . . ." is this one of those?

(4) A few years ago the list had a discussion of W vs. LaB6, vs.
FEG/Schottky for SEM´s and the consensus basically was that LaB6 was
not worth the cost for SEM. (Either FEG if affordable or tungsten
with better bells & whistles). Is LaB6 worth it in terms of TEM?

(5) What more important issue have we neglected in our naivete?

(Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?)

Thank you for any info you're willing to share!



Richard E. Edelmann, Ph.D.
EXPO Editor, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu



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From: bozhilov-at-ucr.edu
Date: Tue, 19 Jun 2007 12:02:16 -0500
Subject: [Microscopy] Re: 200KeV TEM users Opinions, please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In an universal applications environment the only good reason to buy
120kV vs 200kV TEM is lack of money.

===================================
Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

tel. 951 827 2998
fax 951 827 2489
===================================


On Jun 19, 2007, at 9:12 AM, edelmare-at-muohio.edu wrote:

}
}
}
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} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
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} MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
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}
} 200Kev scope users:
}
} O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with
} LaB6 guns. Our biggest concerns come in routine usability, and I am
} hoping to hear any comments on any of the following questions we
} have.
}
} We handle a wide range of samples (Biological thin sections, organic
} macro-molecules, geologic particles, geologic thin sections,
} crystals, thin films, nano-particles, organic and non-organic
} materials) with a wide range of techniques (BF, DF, Diffraction, EDS,
} EELS, including lattice fringe imaging, STEM and tomography
} secondarily). In balancing contrast, resolution, and beam damage we
} really expect to perform a majority of our imaging between 80KeV and
} 120KeV (or 150KeV). However, the option of higher voltages up to
} 200KeV, for higher resolution imaging is something we are
} considering. So we are looking at both 120 and 200 KeV scopes. (And
} yes, we will test as many samples on any scope as we can but a couple
} of days worth of playing with a handful of samples does not compare
} to years worth of experience.)
}
} (1) It seems that most 200Kev scopes are set to 200Kev and left
} there - presumably because it eases alignments, voltage stability,
} etc. when pushing for ultimate resolution. Is this true? Yes, all
} the manufacturers list voltage ranges the scope can be operated at,
} but does anyone really use anything else? With the digitizing of the
} scope controls and storage of multiple alignment setting, alignment
} at different voltages (theoretically) should be fairly straight
} forward but is it really?
}
} (2) Is 200KeV worth having if the majority of the work does not
} require 200KeV? Not having personally worked much at 200KeV, Does
} loss of contrast and beam damage at 200KeV limit usability when
} imaging other than atomic / lattice level?
}
} (3) If the majority of the scope usage is 80 - 140 KeV is the cost
} of a 200KeV instrument worth it? In terms of initial cost, and
} maintenance. (Even if the only option tops off at 120KeV). We are
} all aware of instances of: "If you can at all afford it get, even if
} you won´t use it regularly, because . . ." is this one of those?
}
} (4) A few years ago the list had a discussion of W vs. LaB6, vs.
} FEG/Schottky for SEM´s and the consensus basically was that LaB6 was
} not worth the cost for SEM. (Either FEG if affordable or tungsten
} with better bells & whistles). Is LaB6 worth it in terms of TEM?
}
} (5) What more important issue have we neglected in our naivete?
}
} (Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?)
}
} Thank you for any info you're willing to share!
}
}
}
} Richard E. Edelmann, Ph.D.
} EXPO Editor, Microscopy and Microanalysis Supplement
} Electron Microscopy Facility Director
} 364 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
}
}
}
} ==============================Original
} Headers==============================
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} 15, 23 -- To: microscopy-at-Microscopy.com
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8, 22 -- Subject: Re: [Microscopy] 200KeV TEM users Opinions, please
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From: bfoster-at-mme1.com
Date: Tue, 19 Jun 2007 14:35:19 -0500
Subject: [Microscopy] Re: Unusual request

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Dear DJ

You don't indicate where you are located.

If you are in the Northeast, get in touch with either John Oren (VT
Optics - 802-425-2040) or Dennis O'Leary (MicroOptical Methods
518-482-8200 or rdenol-at-hotmail.com). Alternatively, Don Thomson (see
CC above) in the UK does a lot of this sort of work. Also, the
Queckett Society in the UK does a lot with old microscopes... Don can
answer more questions about them.

Have FUN!!!

Best regards,
Barbara Foster, President

We're moving July 1!
Microscopy/Microscopy Education
7101 Royal Glen Trail, Suite A
McKinney TX 75070
P: (413)246-2841
W: www.MicroscopyEducation.com


MME is now scheduling customized, on-site courses through
September. Call us today for details.

P. S.
Need a good general reference or light microscopy text for next
semester? Call us today to learn more about "Optimizing LIght
Microscopy". Copies still available through MME... even for
class-room lots ... and we give quantity discounts. Call Ken Piel at
(972)954-8011 until July 1.

At 09:36 AM 6/19/2007, you wrote:



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From: larry-at-celtic.freewire.co.uk
Date: Tue, 19 Jun 2007 15:51:12 -0500
Subject: [Microscopy] RE: 200KeV TEM users Opinions, please

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} -----Original Message-----
} X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu]
} Sent: June 19, 2007 9:18 AM
} To: maryflet-at-interchange.ubc.ca
} Subject: [Microscopy] 200KeV TEM users Opinions, please
}
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I well remember my days as a TEM demonstrator
trying to persuade users, often unsuccessfuly,
that going to higher kV really did reduce damage
.......

However, while for many samples, higher kV does
result in reduced thermal damage, for some
samples knock-on damage begins at surprisingly
low thresholds and this does increase as the kV
increases. A particularly topical example is
carbon nanotubes, where there is evidence that
knock-on damage begins at ~80 kV ...

You also need to remember that with digital CCD
camera systems, image contrast is much less
dependent on kV, so with a good digital camera,
you can get both improved resolution and good
image contrast by operating at 200 kV.

LaB6 is definitely worth using on a TEM. Unlike
SEMs, there is generally no additional cost apart
from the filaments themselves, since the gun
vacuum levels on modern instruments should be
more than sufficient for LaB6. At higher
magnifications, the combination of greater
brightness and smaller spot sizes usually means
that image are brighter. And filament lifetimes
should be significantly longer. LaB6 filaments
also give somewhat better beam coherence, which
in practical terms results in better contrast in
high resolution images.

My feeling is that really the only reason to go
for 100/120 KV is that the budget isn't availabe
for 200 kV.

And while you can always turn the kV down on a
200 kV instrument, you can't turn it up on a
100/120 kV instrument!

--
Larry Stoter
JEOL (UK) Ltd
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoluk.com

PLEASE NOTE - Agressive SPAM filtering is
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From: lhermini-at-epoisses.inra.fr
Date: Tue, 19 Jun 2007 22:43:24 -0500
Subject: [Microscopy] viaWWW: colloidal gold conjugation of proteins

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Email: lhermini-at-epoisses.inra.fr
Name: jeannine Lherminier

Organization: INRA

Title-Subject: [Filtered] colloidal gold conjugation of proteins

Question: I am interested to preprare a protein-gold (5nm) complex to follow internalization of the protein into the cell. I used the protocols described by Hayat (colloidal gold , Principles, Methods and Applications) and previous recommandations of M. Bendayan but I failed... The protein is a small protein of 10kDa with a pHi of 8,5 and is dissolved in water. In parallel, I performed a control BSA-colloidal gold complex and it is OK. I tried to work with a pH of the gold solution slighly basic to the isoelectric pH of the protein, pH=9. I also tried to work at a pH of 7,4 and I also failed. In both cases, I added the colloidal gold (pH=9 or pH=7.4) to the protein solution. As recommended by M. Bendayan, I did not use at this step any PEG or other protective agents. I then centriguged the mixture at 45,000 rpm at 4ƒC for 60 min. The sediment at the bottom is dark-red. I recovered the sediment in PBS containing 0.02% PEG 20000. When I checked with negative staining with TEM, I did not visualise any gold particles or very few!!!.
Is it the molecular weight of the protein a problem? Is the pH of the gold solution not optimal? Is a protective agent has to be used to stabilize the mixture befor centrifugation?
Thanks a lot

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From: allanwojtasp-at-agr.gc.ca
Date: Tue, 19 Jun 2007 22:43:46 -0500
Subject: [Microscopy] viaWWW: Can a tissue fixation protocol be patented?

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Email: allanwojtasp-at-agr.gc.ca
Name: Paula Allan-Wojtas

Organization: Agriculture and Agri-Food Canada

Title-Subject: [Filtered] Can a tissue fixation protocol be patented?

Question: I have just heard that a company has just patented a tissue fixation protocol. Is this possible? It doesn't sound correct to me, since a number of papers have been published in the scientific literature over the past 10 years which have used this particular protocol already. Can this really happen? What are the repercussions if this is true?



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From: munhutup1-at-gmail.com
Date: Tue, 19 Jun 2007 22:44:11 -0500
Subject: [Microscopy] viaWWW: Segmentation of particles in Gatan Digital Micrograph

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Email: munhutup1-at-gmail.com
Name: Paidemwoyo Munhutu

Organization: Southern Connecticut State University

Title-Subject: [Filtered] RE: Segmentation of particles in Gatan Digital Micrograph Software

Question: Hello,

I am an undergraduate student at Southern Connecticut State University involved in research. I was wondering if there was any way of segmenting clustered particles in Gatan. I know that you can apply a watershed algorithm to binary images in ImageJ, but I am looking for the equivalent in Gatan. I am also open to any scripts that I can use to apply segmentation to the images within Gatan.

Many Thanks

Paida


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From: edelmare-at-Muohio.edu
Date: Wed, 20 Jun 2007 07:23:45 -0500
Subject: [Microscopy] Re: 200KeV TEM users Opinions, please

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Krassimir:

Then you have no problem running any KeV at any time in your TEM(s)?


On 19 Jun 2007 at 10:02, K.N. Bozhilov wrote:

} In an universal applications environment the only good reason to buy
} 120kV vs 200kV TEM is lack of money.
}
} ===================================
} Krassimir N. Bozhilov
} Central Facility for Advanced Microscopy and Microanalysis
} University of California
} Riverside, CA 92521
}
} tel. 951 827 2998
} fax 951 827 2489
} ===================================
}
}
} On Jun 19, 2007, at 9:12 AM, edelmare-at-muohio.edu wrote:
}
} }
} }
} }
} } ----------------------------------------------------------------------
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} }
} } 200Kev scope users:
} }
} } O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with
} } LaB6 guns. Our biggest concerns come in routine usability, and I am
} } hoping to hear any comments on any of the following questions we
} } have.
} }
} } We handle a wide range of samples (Biological thin sections, organic
} } macro-molecules, geologic particles, geologic thin sections,
} } crystals, thin films, nano-particles, organic and non-organic
} } materials) with a wide range of techniques (BF, DF, Diffraction, EDS,
} } EELS, including lattice fringe imaging, STEM and tomography
} } secondarily). In balancing contrast, resolution, and beam damage we
} } really expect to perform a majority of our imaging between 80KeV and
} } 120KeV (or 150KeV). However, the option of higher voltages up to
} } 200KeV, for higher resolution imaging is something we are
} } considering. So we are looking at both 120 and 200 KeV scopes. (And
} } yes, we will test as many samples on any scope as we can but a couple
} } of days worth of playing with a handful of samples does not compare
} } to years worth of experience.)
} }
} } (1) It seems that most 200Kev scopes are set to 200Kev and left
} } there - presumably because it eases alignments, voltage stability,
} } etc. when pushing for ultimate resolution. Is this true? Yes, all
} } the manufacturers list voltage ranges the scope can be operated at,
} } but does anyone really use anything else? With the digitizing of the
} } scope controls and storage of multiple alignment setting, alignment
} } at different voltages (theoretically) should be fairly straight
} } forward but is it really?
} }
} } (2) Is 200KeV worth having if the majority of the work does not
} } require 200KeV? Not having personally worked much at 200KeV, Does
} } loss of contrast and beam damage at 200KeV limit usability when
} } imaging other than atomic / lattice level?
} }
} } (3) If the majority of the scope usage is 80 - 140 KeV is the cost
} } of a 200KeV instrument worth it? In terms of initial cost, and
} } maintenance. (Even if the only option tops off at 120KeV). We are
} } all aware of instances of: "If you can at all afford it get, even if
} } you won´t use it regularly, because . . ." is this one of those?
} }
} } (4) A few years ago the list had a discussion of W vs. LaB6, vs.
} } FEG/Schottky for SEM´s and the consensus basically was that LaB6 was
} } not worth the cost for SEM. (Either FEG if affordable or tungsten
} } with better bells & whistles). Is LaB6 worth it in terms of TEM?
} }
} } (5) What more important issue have we neglected in our naivete?
} }
} } (Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?)
} }
} } Thank you for any info you're willing to share!
} }
} }
} }
} } Richard E. Edelmann, Ph.D.
} } EXPO Editor, Microscopy and Microanalysis Supplement
} } Electron Microscopy Facility Director
} } 364 Pearson Hall
} } Miami University, Oxford, OH 45056
} } Ph: 513.529.5712 Fax: 513.529.4243
} } E-mail: edelmare-at-muohio.edu
} } http://www.emf.muohio.edu
} }
} }
} }
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Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."



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From: rdsouza78-at-hotmail.com
Date: Wed, 20 Jun 2007 08:23:48 -0500
Subject: [Microscopy] viaWWW: Inverted gelatin capsule cryosections

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Name: Ryan D'Souza

Organization: National Institute For Research In Reproductive Health

Title-Subject: [Filtered] Inverted gelatin capsule cryosections

Question: I would like to perform an immunohistochemical analysis on cryosections ( with DAB) and then process them for TEM. I have come across several articles that use the INVERTED GELATIN CAPSULE METHOD. i would appreciate if some one could give me an insight/ detailed protocol on how the technique is done.

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: nizets2-at-yahoo.com
Date: Wed, 20 Jun 2007 09:15:21 -0500
Subject: [Microscopy] Re: tissue storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi!

If I remember well this topic was discussed some time
ago and it was said that was ok in cacodylate buffer
as long as this is not stored at 37°C ;-)
My 5 cent: perhaps some calcium could help too.

regards,

Stephane

--- skperkin-at-vt.edu wrote:

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}
}
} Hello-
}
} Does anyone have experience storing
} glutaraldehyde-fixed tissues for potential
} TEM in 0.1M cacodylate buffer, 5% sucrose and
} thiomersal? Tissues may be
} stored for up to 3 months.
}
} Thanks in advance!
} Sandy Hancock
}
}
} VMRCVM
} Virginia Tech
}
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} Headers==============================
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} 2007
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==============================Original Headers==============================
9, 21 -- From nizets2-at-yahoo.com Wed Jun 20 09:15:21 2007
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From: nizets2-at-yahoo.com
Date: Wed, 20 Jun 2007 09:40:33 -0500
Subject: [Microscopy] Re: 200KeV TEM users Opinions, please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Richard!

I will join the opinion of previous posters of the
list.
A LaB6 at 200keV just offers more flexibility for no
disadvantages except the cost (I don't know the
difference with W gun). With my TEM I can even work at
1 keV if I want to(which I doesn't ;-))
And I can store all the settings I want at any keV or
any magnification I choose (you can still ask the
company about these important "details").
I would add that if you plan to work on nanoparticles
you will probably appreciate the additional comfort of
a LaB6 working at 200keV at high mag.

Best regards,

Stephane







--- edelmare-at-muohio.edu wrote:

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}
} 200Kev scope users:
}
} O.k., we are trying to evaluate 120Kev TEM´s vs
} 200Kev TEM´s with
} LaB6 guns. Our biggest concerns come in routine
} usability, and I am
} hoping to hear any comments on any of the following
} questions we
} have.
}
} We handle a wide range of samples (Biological thin
} sections, organic
} macro-molecules, geologic particles, geologic thin
} sections,
} crystals, thin films, nano-particles, organic and
} non-organic
} materials) with a wide range of techniques (BF, DF,
} Diffraction, EDS,
} EELS, including lattice fringe imaging, STEM and
} tomography
} secondarily). In balancing contrast, resolution,
} and beam damage we
} really expect to perform a majority of our imaging
} between 80KeV and
} 120KeV (or 150KeV). However, the option of higher
} voltages up to
} 200KeV, for higher resolution imaging is something
} we are
} considering. So we are looking at both 120 and 200
} KeV scopes. (And
} yes, we will test as many samples on any scope as we
} can but a couple
} of days worth of playing with a handful of samples
} does not compare
} to years worth of experience.)
}
} (1) It seems that most 200Kev scopes are set to
} 200Kev and left
} there - presumably because it eases alignments,
} voltage stability,
} etc. when pushing for ultimate resolution. Is this
} true? Yes, all
} the manufacturers list voltage ranges the scope can
} be operated at,
} but does anyone really use anything else? With the
} digitizing of the
} scope controls and storage of multiple alignment
} setting, alignment
} at different voltages (theoretically) should be
} fairly straight
} forward but is it really?
}
} (2) Is 200KeV worth having if the majority of the
} work does not
} require 200KeV? Not having personally worked much
} at 200KeV, Does
} loss of contrast and beam damage at 200KeV limit
} usability when
} imaging other than atomic / lattice level?
}
} (3) If the majority of the scope usage is 80 - 140
} KeV is the cost
} of a 200KeV instrument worth it? In terms of
} initial cost, and
} maintenance. (Even if the only option tops off at
} 120KeV). We are
} all aware of instances of: "If you can at all
} afford it get, even if
} you won´t use it regularly, because . . ." is this
} one of those?
}
} (4) A few years ago the list had a discussion of W
} vs. LaB6, vs.
} FEG/Schottky for SEM´s and the consensus basically
} was that LaB6 was
} not worth the cost for SEM. (Either FEG if
} affordable or tungsten
} with better bells & whistles). Is LaB6 worth it in
} terms of TEM?
}
} (5) What more important issue have we neglected in
} our naivete?
}
} (Boy, wouldn´t a 150KeV Schottky-FEG be a nice
} compromise?)
}
} Thank you for any info you're willing to share!
}
}
}
} Richard E. Edelmann, Ph.D.
} EXPO Editor, Microscopy and Microanalysis Supplement
} Electron Microscopy Facility Director
} 364 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
}
}
}
} ==============================Original
} Headers==============================
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} 11:09:28 2007
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==============================Original Headers==============================
17, 21 -- From nizets2-at-yahoo.com Wed Jun 20 09:40:32 2007
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17, 21 -- Subject: Re: [Microscopy] 200KeV TEM users Opinions, please
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From: thoward-at-unm.edu
Date: Wed, 20 Jun 2007 09:47:19 -0500
Subject: [Microscopy] Re: viaWWW: Can a tissue fixation protocol be

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well, this kind of thing has been done in the past -
wasn't the tyramide amplification technique originally
published by a research group, then patented by a company?
I'm sure there are other instances; this one comes to mind
because there were discussions of it at the time on one of
the on-line groups - maybe this one?

Anyway - which fixation?

Tamara

On Tue, 19 Jun 2007 22:45:33 -0500
allanwojtasp-at-agr.gc.ca wrote:
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} Email: allanwojtasp-at-agr.gc.ca
} Name: Paula Allan-Wojtas
}
} Organization: Agriculture and Agri-Food Canada
}
} Title-Subject: [Filtered] Can a tissue fixation protocol
} be patented?
}
} Question: I have just heard that a company has just
} patented a tissue fixation protocol. Is this possible? It
} doesn't sound correct to me, since a number of papers
} have been published in the scientific literature over the
} past 10 years which have used this particular protocol
} already. Can this really happen? What are the
} repercussions if this is true?
}
}
}
} ---------------------------------------------------------------------------
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} ==============================Original
} Headers==============================
} 7, 11 -- From zaluzec-at-microscopy.com Tue Jun 19 22:43:45
} 2007
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} (mac22.zaluzec.com [206.69.208.22])
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} 7, 11 -- From: allanwojtasp-at-agr.gc.ca (by way of
} MicroscopyListserver)
} 7, 11 -- Subject: viaWWW: Can a tissue fixation protocol
} be patented?
} 7, 11 -- Content-Type: text/plain; charset="us-ascii"
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***************************
Tamara Howard
Cell Biology & Physiology
UNM-HSC
Albuquerque, NM
***************************

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From: oshel1pe-at-cmich.edu
Date: Wed, 20 Jun 2007 10:32:16 -0500
Subject: [Microscopy] Fwd: Re: Fwd: viaWWW: colloidal gold conjugation of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Date: Wed, 20 Jun 2007 10:05:30 -0500
} From: Daryl Meyer {dameyer-at-wisc.edu}
} Subject: Re: Fwd: [Microscopy] viaWWW: colloidal gold conjugation of proteins
} To: Philip Oshel {oshel1pe-at-cmich.edu}
}
} Phil, I just subscribed, but it looks like it
} may take a day to be able to access the list, so
} I'll let you forward my response:
}
} I don't think the problem lies with the MW of
} the protein or the pH of 9 for conjugation.
}
} To better address the question, I need a little
} more info. First, was a concentration isotherm
} performed at either pH to determine the amount
} of protein necessary to stabilize a given volume
} of cAu against electrolytic floculation (ie,
} influx of saturated NaCl)? This by itself will
} go a long way in telling whether or not stable
} conjugates are present. If the conjugates remain
} red in color above a certain protein
} concentration following addition of salt, then
} it would seem that the reason very few particles
} are visualized by TEM is that either the
} sedimented conjugates were too dilute following
} resuspension, or they were not allowed to adhere
} to the grids for a long enough period of time.
} For labeling experiments, we generally resuspend
} to one-tenth of the original volume of cAu,
} giving a particle concentration of roughly 10^13
} per ml. We've found this to effectively minimize
} the amount of time required to saturate all
} binding sites on a cell surface. At this
} concentration,
} a grid should only need to sit on a drop of the
} conjugate for 20 minutes or so at room temp for
} there to be plenty of particles for imaging.
}
} To visualize the protein coat around the
} particles by negative staining, the PEG has to
} be omitted entirely in order to be sure that
} whatever surrounds the particles is definitely
} protein and not PEG. If the conjugates are not
} stable in PBS without PEG, then resuspend in
} water to do the negative staining. If PEG is
} needed as a secondary stabilizer in PBS for the
} labeling, I add it after conjugation, but before
} centrifugation. But remember that it's important
} to do the initial concentration isotherm in
} order to be sure that protein is bound to the
} particles, and that it's not simply the PEG
} that's stabilizing them.
}
} I hope this is helpful.
}
} Daryl
} } } } } Date: Tue, 19 Jun 2007 22:50:28 -0500
} } } } } To: oshel1pe-at-cmich.edu
} } } } } From: lhermini-at-epoisses.inra.fr
} } } } } Reply-to: lhermini-at-epoisses.inra.fr
} } } }
} } } ----------------------------------------------------------------------------
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} } } } } Email: lhermini-at-epoisses.inra.fr
} } } } } Name: jeannine Lherminier
} } } } }
} } } } } Organization: INRA
} } } } }
} } } } } Title-Subject: [Filtered] colloidal gold conjugation of proteins
} } } } }
} } } } } Question: I am interested to preprare a
} } } } } protein-gold (5nm) complex to follow
} } } } } internalization of the protein into the cell. I
} } } } } used the protocols described by Hayat (colloidal
} } } } } gold , Principles, Methods and Applications) and
} } } } } previous recommandations of M. Bendayan but I
} } } } } failed... The protein is a small protein of
} } } } } 10kDa with a pHi of 8,5 and is dissolved in
} } } } } water. In parallel, I performed a control
} } } } } BSA-colloidal gold complex and it is OK. I tried
} } } } } to work with a pH of the gold solution slighly
} } } } } basic to the isoelectric pH of the protein,
} } } } } pH=9. I also tried to work at a pH of 7,4 and I
} } } } } also failed. In both cases, I added the
} } } } } colloidal gold (pH=9 or pH=7.4) to the protein
} } } } } solution. As recommended by M. Bendayan, I did
} } } } } not use at this step any PEG or other protective
} } } } } agents. I then centriguged the mixture at 45,000
} } } } } rpm at 4ÉC for 60 min. The sediment at the
} } } } } bottom is dark-red. I recovered the sediment in
} } } } } PBS containing 0.02% PEG 20000. When I checked
} } } } } with negative staining with TE!
} } } } } M, I did not visualise any gold particles or very few!!!.
} } } } } Is it the molecular weight of the protein a
} } } } } problem? Is the pH of the gold solution not
} } } } } optimal? Is a protective agent has to be used to
} } } } } stabilize the mixture befor centrifugation?
} } } } } Thanks a lot
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859


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From: bozhilov-at-ucr.edu
Date: Wed, 20 Jun 2007 10:56:32 -0500
Subject: [Microscopy] 200KeV TEM users Opinions, please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard,

Yes, there is no problem running at any kV one desires.

Furthermore the quality of images at higher voltages is far superior
due to the better coherence of the el. beam for the LaB6 cathode and
decreased inelastic scattering compared to tungsten and low voltage.

Also the need of lower voltage for improved contrast is questionable
if the system is equipped with digital imaging capability. As long as
resolution is there contrast can be improved quite significantly just
by post-acquisition processing.

Radiolysis is the main problem causing specimen damage at voltages
less than 300 kV and this increases with decreasing accelerating
voltage. That is another advantage to operate at higher voltages.

The only drawback for having higher voltage instrument with LaB6 is
the stricter vacuum requirements.
For many life science applications this my cause some slowdown since
pumping times will be longer during sample exchange. This can be
resolved by obtaining multiple-grid specimen holders.

I would even suggest if funding is not a problem to go for a 300 kV
instrument, provided you expect to do substantial work in materials
science and geology.

Krassimir.

===================================
Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

tel. 951 827 2998
fax 951 827 2489
===================================


On Jun 20, 2007, at 5:27 AM, edelmare-at-Muohio.edu wrote:

}
}
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} Krassimir:
}
} Then you have no problem running any KeV at any time in your TEM(s)?
}
}
} On 19 Jun 2007 at 10:02, K.N. Bozhilov wrote:
}
} } In an universal applications environment the only good reason to buy
} } 120kV vs 200kV TEM is lack of money.
} }
} } ===================================
} } Krassimir N. Bozhilov
} } Central Facility for Advanced Microscopy and Microanalysis
} } University of California
} } Riverside, CA 92521
} }
} } tel. 951 827 2998
} } fax 951 827 2489
} } ===================================
} }
} }
} } On Jun 19, 2007, at 9:12 AM, edelmare-at-muohio.edu wrote:
} }
} } }
} } }
} } }
} } } --------------------------------------------------------------------
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} } }
} } } 200Kev scope users:
} } }
} } } O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with
} } } LaB6 guns. Our biggest concerns come in routine usability, and I am
} } } hoping to hear any comments on any of the following questions we
} } } have.
} } }
} } } We handle a wide range of samples (Biological thin sections, organic
} } } macro-molecules, geologic particles, geologic thin sections,
} } } crystals, thin films, nano-particles, organic and non-organic
} } } materials) with a wide range of techniques (BF, DF, Diffraction,
} } } EDS,
} } } EELS, including lattice fringe imaging, STEM and tomography
} } } secondarily). In balancing contrast, resolution, and beam damage we
} } } really expect to perform a majority of our imaging between 80KeV and
} } } 120KeV (or 150KeV). However, the option of higher voltages up to
} } } 200KeV, for higher resolution imaging is something we are
} } } considering. So we are looking at both 120 and 200 KeV scopes.
} } } (And
} } } yes, we will test as many samples on any scope as we can but a
} } } couple
} } } of days worth of playing with a handful of samples does not compare
} } } to years worth of experience.)
} } }
} } } (1) It seems that most 200Kev scopes are set to 200Kev and left
} } } there - presumably because it eases alignments, voltage stability,
} } } etc. when pushing for ultimate resolution. Is this true? Yes, all
} } } the manufacturers list voltage ranges the scope can be operated at,
} } } but does anyone really use anything else? With the digitizing of
} } } the
} } } scope controls and storage of multiple alignment setting, alignment
} } } at different voltages (theoretically) should be fairly straight
} } } forward but is it really?
} } }
} } } (2) Is 200KeV worth having if the majority of the work does not
} } } require 200KeV? Not having personally worked much at 200KeV, Does
} } } loss of contrast and beam damage at 200KeV limit usability when
} } } imaging other than atomic / lattice level?
} } }
} } } (3) If the majority of the scope usage is 80 - 140 KeV is the cost
} } } of a 200KeV instrument worth it? In terms of initial cost, and
} } } maintenance. (Even if the only option tops off at 120KeV). We are
} } } all aware of instances of: "If you can at all afford it get,
} } } even if
} } } you won´t use it regularly, because . . ." is this one of those?
} } }
} } } (4) A few years ago the list had a discussion of W vs. LaB6, vs.
} } } FEG/Schottky for SEM´s and the consensus basically was that LaB6
} } } was
} } } not worth the cost for SEM. (Either FEG if affordable or tungsten
} } } with better bells & whistles). Is LaB6 worth it in terms of TEM?
} } }
} } } (5) What more important issue have we neglected in our naivete?
} } }
} } } (Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?)
} } }
} } } Thank you for any info you're willing to share!
} } }
} } }
} } }
} } } Richard E. Edelmann, Ph.D.
} } } EXPO Editor, Microscopy and Microanalysis Supplement
} } } Electron Microscopy Facility Director
} } } 364 Pearson Hall
} } } Miami University, Oxford, OH 45056
} } } Ph: 513.529.5712 Fax: 513.529.4243
} } } E-mail: edelmare-at-muohio.edu
} } } http://www.emf.muohio.edu
} } }
} } }
} } }
} } } ==============================Original
} } } Headers==============================
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}
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Director
} 364 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
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} "RAM disk is NOT an installation procedure."
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} microscopy-at-microscopy.com
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==============================Original Headers==============================
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From: phillipst-at-missouri.edu
Date: Wed, 20 Jun 2007 11:01:19 -0500
Subject: [Microscopy] viaWWW: Can a tissue fixation protocol be

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am no lawyer but there are 3 facts that I have heard repeatedly.

First, if one doesn't patent (or start the process) within 12 months of it
being published, it can't be.

Second, a patent only gives someone the right to sue to try and prove they
own the rights. A court case can invalidate it - in other words, getting a
patent isn't considered proof that you deserved it.

Third, one can't patent a process that anyone working in the field would or
could do without any special thought or design. Karnovsky's idea to combine
paraformaldehyde and glutaraldehyde probably wasn't patentable.

If someone comes up with a new fixative using a novel chemical, that
probably would be patentable.

But I am curious, what fixative and where did you see it was patented.

At 09:47 AM 06/20/07, you wrote:



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Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

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==============================Original Headers==============================
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From: john.mardinly-at-intel.com
Date: Wed, 20 Jun 2007 14:52:32 -0500
Subject: [Microscopy] 200KeV TEM users Opinions, please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard, Krassimir;
Please let me contribute one aspect to this thread that has not been considered: unless you purchase a TEM with constant power lenses, and I am not aware of any other than the FEI Titan, you will have a lengthy period of thermal instability following an accelerating voltage change because the lens currents change significantly. Dropping accelerating voltage can result in over-cooling the column, which can result in condensation, vacuum leaks and thermal drift, even if the water flow is adjusted. Raising the accelerating voltage after an extended period of low-voltage operation can result in a need to condition the accelerator to avoid HT instability. Those are some of the other reasons that TEMs generally run at their rated high voltage.

John Mardinly
Intel Corporation

This comment does not represent an opinion of Intel Corporation.

-----Original Message-----
X-from: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu]
Sent: Wednesday, June 20, 2007 8:57 AM
To: Mardinly, John

Richard,

Yes, there is no problem running at any kV one desires.

Furthermore the quality of images at higher voltages is far superior
due to the better coherence of the el. beam for the LaB6 cathode and
decreased inelastic scattering compared to tungsten and low voltage.

Also the need of lower voltage for improved contrast is questionable
if the system is equipped with digital imaging capability. As long as
resolution is there contrast can be improved quite significantly just
by post-acquisition processing.

Radiolysis is the main problem causing specimen damage at voltages
less than 300 kV and this increases with decreasing accelerating
voltage. That is another advantage to operate at higher voltages.

The only drawback for having higher voltage instrument with LaB6 is
the stricter vacuum requirements.
For many life science applications this my cause some slowdown since
pumping times will be longer during sample exchange. This can be
resolved by obtaining multiple-grid specimen holders.

I would even suggest if funding is not a problem to go for a 300 kV
instrument, provided you expect to do substantial work in materials
science and geology.

Krassimir.

===================================
Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

tel. 951 827 2998
fax 951 827 2489
===================================


On Jun 20, 2007, at 5:27 AM, edelmare-at-Muohio.edu wrote:

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} Krassimir:
}
} Then you have no problem running any KeV at any time in your TEM(s)?
}
}
} On 19 Jun 2007 at 10:02, K.N. Bozhilov wrote:
}
} } In an universal applications environment the only good reason to buy
} } 120kV vs 200kV TEM is lack of money.
} }
} } ===================================
} } Krassimir N. Bozhilov
} } Central Facility for Advanced Microscopy and Microanalysis
} } University of California
} } Riverside, CA 92521
} }
} } tel. 951 827 2998
} } fax 951 827 2489
} } ===================================
} }
} }
} } On Jun 19, 2007, at 9:12 AM, edelmare-at-muohio.edu wrote:
} }
} } }
} } }
} } }
} } } --------------------------------------------------------------------
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} } }
} } } 200Kev scope users:
} } }
} } } O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with
} } } LaB6 guns. Our biggest concerns come in routine usability, and I am
} } } hoping to hear any comments on any of the following questions we
} } } have.
} } }
} } } We handle a wide range of samples (Biological thin sections, organic
} } } macro-molecules, geologic particles, geologic thin sections,
} } } crystals, thin films, nano-particles, organic and non-organic
} } } materials) with a wide range of techniques (BF, DF, Diffraction,
} } } EDS,
} } } EELS, including lattice fringe imaging, STEM and tomography
} } } secondarily). In balancing contrast, resolution, and beam damage we
} } } really expect to perform a majority of our imaging between 80KeV and
} } } 120KeV (or 150KeV). However, the option of higher voltages up to
} } } 200KeV, for higher resolution imaging is something we are
} } } considering. So we are looking at both 120 and 200 KeV scopes.
} } } (And
} } } yes, we will test as many samples on any scope as we can but a
} } } couple
} } } of days worth of playing with a handful of samples does not compare
} } } to years worth of experience.)
} } }
} } } (1) It seems that most 200Kev scopes are set to 200Kev and left
} } } there - presumably because it eases alignments, voltage stability,
} } } etc. when pushing for ultimate resolution. Is this true? Yes, all
} } } the manufacturers list voltage ranges the scope can be operated at,
} } } but does anyone really use anything else? With the digitizing of
} } } the
} } } scope controls and storage of multiple alignment setting, alignment
} } } at different voltages (theoretically) should be fairly straight
} } } forward but is it really?
} } }
} } } (2) Is 200KeV worth having if the majority of the work does not
} } } require 200KeV? Not having personally worked much at 200KeV, Does
} } } loss of contrast and beam damage at 200KeV limit usability when
} } } imaging other than atomic / lattice level?
} } }
} } } (3) If the majority of the scope usage is 80 - 140 KeV is the cost
} } } of a 200KeV instrument worth it? In terms of initial cost, and
} } } maintenance. (Even if the only option tops off at 120KeV). We are
} } } all aware of instances of: "If you can at all afford it get,
} } } even if
} } } you won´t use it regularly, because . . ." is this one of those?
} } }
} } } (4) A few years ago the list had a discussion of W vs. LaB6, vs.
} } } FEG/Schottky for SEM´s and the consensus basically was that LaB6
} } } was
} } } not worth the cost for SEM. (Either FEG if affordable or tungsten
} } } with better bells & whistles). Is LaB6 worth it in terms of TEM?
} } }
} } } (5) What more important issue have we neglected in our naivete?
} } }
} } } (Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?)
} } }
} } } Thank you for any info you're willing to share!
} } }
} } }
} } }
} } } Richard E. Edelmann, Ph.D.
} } } EXPO Editor, Microscopy and Microanalysis Supplement
} } } Electron Microscopy Facility Director
} } } 364 Pearson Hall
} } } Miami University, Oxford, OH 45056
} } } Ph: 513.529.5712 Fax: 513.529.4243
} } } E-mail: edelmare-at-muohio.edu
} } } http://www.emf.muohio.edu
} } }
} } }
} } }
} } } ==============================Original
} } } Headers==============================
} } } 15, 23 -- From edelmare-at-muohio.edu Tue Jun 19 11:09:28 2007
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} } } 15, 23 -- To: microscopy-at-Microscopy.com
} } } 15, 23 -- Date: Tue, 19 Jun 2007 12:09:26 -0400
} } } 15, 23 -- MIME-Version: 1.0
} } } 15, 23 -- Subject: 200KeV TEM users Opinions, please
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} }
}
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Director
} 364 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}
}
} ==============================Original
} Headers==============================
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} microscopy-at-microscopy.com
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From: WAHeeschen-at-dow.com
Date: Wed, 20 Jun 2007 14:58:19 -0500
Subject: [Microscopy] Looking for parts for Reichert KF-80 cryo-freezer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings:
We are looking to obtain/purchase a used ethane disposal system for a
Reichert KF-80 cryo-freezing unit. The vendor is no longer able to
supply one directly, so we are looking into the used market. If you
have an ethane disposal system that you no longer use and would like to
get rid of it, please send me a quick e-mail so we can discuss off-line:
waheeschen-at-dow.com
Best Regards,

Bill

William A. Heeschen, Ph.D.
Microscopy, Digital Imaging
The Dow Chemical Company
Midland, MI 48674
waheeschen-at-dow.com


==============================Original Headers==============================
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From: sharon.goresh-at-basf.com
Date: Wed, 20 Jun 2007 17:51:52 -0500
Subject: [Microscopy] viaWWW: Open Position BASF

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This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
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Email: sharon.goresh-at-basf.com
Name: Sharon L. Goresh

Organization: BASF Catalysts LLC

Title-Subject: [Filtered] Open Position BASF

Question: Chemist ñ 0700864

Description -
Materials Characterization supports BASF Catalysts R&D by providing characterization, solving technical problems and participating in a wide range of R&D projects. ÝThis position is vital in our efforts in the key areas of electron microscopy (EPMA and SEM) and metallographic and ceramic sample preparation.

The candidate will spend the majority of their time working in the EPMA lab and the remainder in the sample preparation area. ÝThe successful candidate, with training as needed, will:

1. Prepare samples and operate the Cameca SX-50 Electron Microprobe. Ý
2. Acquire and process data using UNIX and PC based computers
3. Communicate with customers to assess appropriate analytical techniques.
4. Interpret data and issue written reports of the results.

Qualifications ñ
Position requires a BA/BS degree in a scientific discipline with 1-2 years industrial or academic research laboratory experience. ÝHands-on experience using electron microscopes with X-Ray microanalysis capabilities is a plus. ÝThe successful candidate must have a theoretical understanding of inorganic chemistry, materials science or mineralogy and an enthusiasm to learn new techniques. ÝHe/she should also have excellent communication skills, a talent for developing positive working relationships, and the ability to handle a high volume of requests and meet critical deadlines.


Profile -
ÝLocation: New Jersey-Iselin
ÝJob Type: Standard
ÝShift: Day Job



Those interested must apply directly on-line by going to:

http://www.basf.com/careers/taleo/joblist.htm

enter the job number in the search field and then follow the instructions

Chemist ñ 0700864


---------------------------------------------------------------------------


==============================Original Headers==============================
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From: cgarber-at-2spi.com
Date: Wed, 20 Jun 2007 21:24:29 -0500
Subject: [Microscopy] Reply to "unusual request"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

D. Jones wrote:
=============================================

OK, I've been looking around for a group that talks/works on/etc. older
microscopes. But I have not been successful in finding such a group.



I have just picked up an old Olympus PMG metallograph. It is an
absolutely fascinating piece of optical equipment. I've never seen one
like it.

Anyway, as one of my (ever increasing number of) extra-curricula
activities, I've decided I'd like to either restore this instrument,
or alter it so that I can actually use it as a modern metallograph with
digital imaging capabilities. The first option would be my first choice,
but if I can't get the parts needed to do that, I'll shoot for the
second option...



My question to all the extraordinary minds on this group, does anyone
know of someone that works on old microscopes? Does anyone know of a
society or a group that discusses them, has old technical information
about them, things like that?



I apologize if this is way off topic for this group. Please respond
directly to me as I doubt the responses would be of general interest. If
anyone does wish for a list of what I find, I'll be happy to share it,
just let me know.

==============================================

The New York Microscopical Society, see URL

http://www.nyms.org/

might be just what you need. The Society actually owns and maintains
quite a collection of old microscopes, some going back well over 100
years. I would expect that someone associated with this collection of
old microscopes might know who could help you.



Chuck



===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




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From: ph2-at-sprynet.com
Date: Wed, 20 Jun 2007 22:13:21 -0500
Subject: [Microscopy] Reply to "unusual request"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There is one.

I had an application a few years ago to a small group/association (in US).
I met one of the founders who did a presentation on old scopes 3-4 years ago
but don't recall the name (senior moment at an early age). I'll dig up the
info and post it in the next week or so.

I also know of a few (retired) fellows that can and will work on scopes -
I'll send a couple of names off-line. I restore one here or there for my
own sake (old petrographic and biological ones) but don't have time for
outside work.

Tony

......................................................................
Andrew Anthony "Tony" Havics, CHMM, CIH, PE
pH2, LLC
5250 E US 36, Suite 830
Avon, IN 46123
(317) 718-7020 off
(317) 718-7038 fax
(317) 409-3238 cell

90% of Risk Management is knowing where to place the decimal point...any
consultant can give you the other 10%(SM)

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-----Original Message-----
X-from: cgarber-at-2spi.com [mailto:cgarber-at-2spi.com]
Sent: Wednesday, June 20, 2007 10:33 PM
To: ph2-at-sprynet.com

D. Jones wrote:
=============================================

OK, I've been looking around for a group that talks/works on/etc. older
microscopes. But I have not been successful in finding such a group.



I have just picked up an old Olympus PMG metallograph. It is an
absolutely fascinating piece of optical equipment. I've never seen one
like it.

Anyway, as one of my (ever increasing number of) extra-curricula
activities, I've decided I'd like to either restore this instrument,
or alter it so that I can actually use it as a modern metallograph with
digital imaging capabilities. The first option would be my first choice,
but if I can't get the parts needed to do that, I'll shoot for the
second option...



My question to all the extraordinary minds on this group, does anyone
know of someone that works on old microscopes? Does anyone know of a
society or a group that discusses them, has old technical information
about them, things like that?



I apologize if this is way off topic for this group. Please respond
directly to me as I doubt the responses would be of general interest. If
anyone does wish for a list of what I find, I'll be happy to share it,
just let me know.

==============================================

The New York Microscopical Society, see URL

http://www.nyms.org/

might be just what you need. The Society actually owns and maintains
quite a collection of old microscopes, some going back well over 100
years. I would expect that someone associated with this collection of
old microscopes might know who could help you.



Chuck



===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
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From: gary-at-gaugler.com
Date: Wed, 20 Jun 2007 23:25:34 -0500
Subject: [Microscopy] Re: Reply to "unusual request"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My suggestion was to subscribe to the usenet.
Specifically, sci.techniques.microscopy which
has many full time microscope repair persons
on-line.

I don't know if the poster did this. So far,
they have not shown up on the usenet.

gary g.


At 06:28 PM 6/20/2007, you wrote:




} ----------------------------------------------------------------------------
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From: RCsencsits-at-lbl.gov
Date: Wed, 20 Jun 2007 23:27:30 -0500
Subject: [Microscopy] 200KeV TEM users Opinions, please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard, John, Krassimir,

Back at Argonne National Lab, we had a JEOL 4000 that was kept at
400kV to high resolution work. It was aligned by the service
engineers at 100, 200, 300 and 400, so recall of alignments was a
push of a few buttons. At lower voltages the lenses run less power
and are much cooler but generally did not affect resolution for most
work. To run at 400kV for HREM imaging the TEM had to sit overnight
to thermally equilibrate. When needed it ran at 100, 200kV. If
someone really needs high resolution stability, set the HT to the
setting wanted the night before; this is easily handled by indicated
the voltage wanted when someone signs up for their time. If a user
really wants resolution they will remember to indicate the desired
voltage. Condensation and vacuum leaks were not a problem.

Best regards,
Roseann

Roseann Csencsits, PhD
Donner TEM Facility Manager
Lawrence Berkeley Lab
Room 365 Donner
510-486-4548

On Jun 20, 2007, at 1:02 PM, john.mardinly-at-intel.com wrote:

}
}
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/
} MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} Richard, Krassimir;
} Please let me contribute one aspect to this thread that has not
} been considered: unless you purchase a TEM with constant power
} lenses, and I am not aware of any other than the FEI Titan, you
} will have a lengthy period of thermal instability following an
} accelerating voltage change because the lens currents change
} significantly. Dropping accelerating voltage can result in over-
} cooling the column, which can result in condensation, vacuum leaks
} and thermal drift, even if the water flow is adjusted. Raising the
} accelerating voltage after an extended period of low-voltage
} operation can result in a need to condition the accelerator to
} avoid HT instability. Those are some of the other reasons that TEMs
} generally run at their rated high voltage.
}
} John Mardinly
} Intel Corporation
}
} This comment does not represent an opinion of Intel Corporation.
}
} -----Original Message-----
} X-from: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu]
} Sent: Wednesday, June 20, 2007 8:57 AM
} To: Mardinly, John
} Subject: [Microscopy] 200KeV TEM users Opinions, please
}
}
}
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
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} ----------------------------------------------------------------------
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}
} Richard,
}
} Yes, there is no problem running at any kV one desires.
}
} Furthermore the quality of images at higher voltages is far superior
} due to the better coherence of the el. beam for the LaB6 cathode and
} decreased inelastic scattering compared to tungsten and low voltage.
}
} Also the need of lower voltage for improved contrast is questionable
} if the system is equipped with digital imaging capability. As long as
} resolution is there contrast can be improved quite significantly just
} by post-acquisition processing.
}
} Radiolysis is the main problem causing specimen damage at voltages
} less than 300 kV and this increases with decreasing accelerating
} voltage. That is another advantage to operate at higher voltages.
}
} The only drawback for having higher voltage instrument with LaB6 is
} the stricter vacuum requirements.
} For many life science applications this my cause some slowdown since
} pumping times will be longer during sample exchange. This can be
} resolved by obtaining multiple-grid specimen holders.
}
} I would even suggest if funding is not a problem to go for a 300 kV
} instrument, provided you expect to do substantial work in materials
} science and geology.
}
} Krassimir.
}
} ===================================
} Krassimir N. Bozhilov
} Central Facility for Advanced Microscopy and Microanalysis
} University of California
} Riverside, CA 92521
}
} tel. 951 827 2998
} fax 951 827 2489
} ===================================
}
}
} On Jun 20, 2007, at 5:27 AM, edelmare-at-Muohio.edu wrote:
}
} }
} }
} }
} } ------
} }
} } Krassimir:
} }
} } Then you have no problem running any KeV at any time in your TEM(s)?
} }
} }
} } On 19 Jun 2007 at 10:02, K.N. Bozhilov wrote:
} }
} } } In an universal applications environment the only good reason to buy
} } } 120kV vs 200kV TEM is lack of money.
} } }
} } } ===================================
} } } Krassimir N. Bozhilov
} } } Central Facility for Advanced Microscopy and Microanalysis
} } } University of California
} } } Riverside, CA 92521
} } }
} } } tel. 951 827 2998
} } } fax 951 827 2489
} } } ===================================
} } }
} } }
} } } On Jun 19, 2007, at 9:12 AM, edelmare-at-muohio.edu wrote:
} } }
} } } }
} } } }
} } } }
} } } } -
} } } } ------
} } } }
} } } } 200Kev scope users:
} } } }
} } } } O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with
} } } } LaB6 guns. Our biggest concerns come in routine usability, and
} } } } I am
} } } } hoping to hear any comments on any of the following questions we
} } } } have.
} } } }
} } } } We handle a wide range of samples (Biological thin sections,
} } } } organic
} } } } macro-molecules, geologic particles, geologic thin sections,
} } } } crystals, thin films, nano-particles, organic and non-organic
} } } } materials) with a wide range of techniques (BF, DF, Diffraction,
} } } } EDS,
} } } } EELS, including lattice fringe imaging, STEM and tomography
} } } } secondarily). In balancing contrast, resolution, and beam
} } } } damage we
} } } } really expect to perform a majority of our imaging between 80KeV
} } } } and
} } } } 120KeV (or 150KeV). However, the option of higher voltages up to
} } } } 200KeV, for higher resolution imaging is something we are
} } } } considering. So we are looking at both 120 and 200 KeV scopes.
} } } } (And
} } } } yes, we will test as many samples on any scope as we can but a
} } } } couple
} } } } of days worth of playing with a handful of samples does not compare
} } } } to years worth of experience.)
} } } }
} } } } (1) It seems that most 200Kev scopes are set to 200Kev and left
} } } } there - presumably because it eases alignments, voltage stability,
} } } } etc. when pushing for ultimate resolution. Is this true? Yes, all
} } } } the manufacturers list voltage ranges the scope can be operated at,
} } } } but does anyone really use anything else? With the digitizing of
} } } } the
} } } } scope controls and storage of multiple alignment setting, alignment
} } } } at different voltages (theoretically) should be fairly straight
} } } } forward but is it really?
} } } }
} } } } (2) Is 200KeV worth having if the majority of the work does not
} } } } require 200KeV? Not having personally worked much at 200KeV, Does
} } } } loss of contrast and beam damage at 200KeV limit usability when
} } } } imaging other than atomic / lattice level?
} } } }
} } } } (3) If the majority of the scope usage is 80 - 140 KeV is the cost
} } } } of a 200KeV instrument worth it? In terms of initial cost, and
} } } } maintenance. (Even if the only option tops off at 120KeV). We
} } } } are
} } } } all aware of instances of: "If you can at all afford it get,
} } } } even if
} } } } you won´t use it regularly, because . . ." is this one of those?
} } } }
} } } } (4) A few years ago the list had a discussion of W vs. LaB6, vs.
} } } } FEG/Schottky for SEM´s and the consensus basically was that LaB6
} } } } was
} } } } not worth the cost for SEM. (Either FEG if affordable or tungsten
} } } } with better bells & whistles). Is LaB6 worth it in terms of TEM?
} } } }
} } } } (5) What more important issue have we neglected in our naivete?
} } } }
} } } } (Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?)
} } } }
} } } } Thank you for any info you're willing to share!
} } } }
} } } }
} } } }
} } } } Richard E. Edelmann, Ph.D.
} } } } EXPO Editor, Microscopy and Microanalysis Supplement
} } } } Electron Microscopy Facility Director
} } } } 364 Pearson Hall
} } } } Miami University, Oxford, OH 45056
} } } } Ph: 513.529.5712 Fax: 513.529.4243
} } } } E-mail: edelmare-at-muohio.edu
} } } } http://www.emf.muohio.edu
} } } }


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From: gary-at-gaugler.com
Date: Thu, 21 Jun 2007 00:16:44 -0500
Subject: [Microscopy] Reply to "unusual request"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Go download Netscape.

http://www.netscape.com

One of the options in it is to go to the usenet.
However, your ISP must be plugged into the usenet
to make this work. The syntax is usually

usenet.yourisp.com

some form it as

usereader.yourisp.com

In all cases, "yourisp" is the name for your ISP.
I "think" that you can get to the groups via
Yahoo and/or Google. I'm not sure. I use Agent
and connect directly via my ISP.

If you have difficulty, I can post a usenet message
for you and forward the responses. This is a very
good group.

gary g.



At 09:02 PM 6/20/2007, you wrote:

} Gary,
}
} I tried to get onto the group you've mentioned, but have not been successful.
}
} I guess I don't know how to do this... I used to get on to these
} usenet groups a number of years ago, but I seem to have lost the
} ability to do so now with only a web browser available to me....
}
} dj
}
} On Wed, 20 Jun 2007, gary-at-gaugler.com wrote:
}
} } Date: Wed, 20 Jun 2007 23:31:52 -0500
} } From: gary-at-gaugler.com
} } To: dljones-at-bestweb.net
} } Subject: [Microscopy] Re: Reply to "unusual request"
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
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14, 21 -- Date: Wed, 20 Jun 2007 22:16:46 -0800
14, 21 -- To: "David L. Jones" {"dljones-at-bestweb.net"-at-bestweb.net}
14, 21 -- From: Gary Gaugler {gary-at-gaugler.com}
14, 21 -- Subject: Re: [Microscopy] Re: Reply to "unusual request"
14, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com}
14, 21 -- In-Reply-To: {Pine.WNT.4.64.0706210100060.180-at-dljtoshiba}
14, 21 -- References: {200706210431.l5L4VqCp019541-at-ns.microscopy.com}
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From: davilla-at-4pi.com
Date: Thu, 21 Jun 2007 00:41:24 -0500
Subject: [Microscopy] Re: Reply to "unusual request"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

http://groups.google.com/group/sci.techniques.microscopy/topics

} Go download Netscape.
}
} http://www.netscape.com
}
} One of the options in it is to go to the usenet.
} However, your ISP must be plugged into the usenet
} to make this work. The syntax is usually
}
} usenet.yourisp.com
}
} some form it as
}
} usereader.yourisp.com
}
} In all cases, "yourisp" is the name for your ISP.
} I "think" that you can get to the groups via
} Yahoo and/or Google. I'm not sure. I use Agent
} and connect directly via my ISP.
}
} If you have difficulty, I can post a usenet message
} for you and forward the responses. This is a very
} good group.
}
} gary g.
}
}
}
} At 09:02 PM 6/20/2007, you wrote:
}
} } Gary,
} }
} } I tried to get onto the group you've mentioned, but have not been successful.
} }
} } I guess I don't know how to do this... I used to get on to these
} } usenet groups a number of years ago, but I seem to have lost the
} } ability to do so now with only a web browser available to me....
} }
} } dj
} }
} } On Wed, 20 Jun 2007, gary-at-gaugler.com wrote:
} }
} } } Date: Wed, 20 Jun 2007 23:31:52 -0500
} } } From: gary-at-gaugler.com
} } } To: dljones-at-bestweb.net
} } } Subject: [Microscopy] Re: Reply to "unusual request"
} } }
} } }
} } }
} } } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com


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From: gary-at-gaugler.com
Date: Thu, 21 Jun 2007 00:52:40 -0500
Subject: [Microscopy] Reply to "unusual request"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Excellent... Thank you for the pointer.
This is another option for accessing the
usenet. It should work.

gary g.



At 09:43 PM 6/20/2007, you wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


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From: nizets2-at-yahoo.com
Date: Thu, 21 Jun 2007 01:41:40 -0500
Subject: [Microscopy] Re: Fixing low pH extremophiles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Tina,

That may sound stupid, but I would still try fixation
at neutral pH! Would you expect the change of pH to
have dramatic effects?
In parallel you could still try to fix at pH 3. Surely
glutaraldehyde won't be at its maximum efficiency but
if you increase the fixation time, it'll probably work
somehow.
Just compare the results at both pHs. And please let
us know what came out, so there is a chance that we
will be less stupid tomorrow than yesterday.

Regards,

Stephane







--- tina-at-pbrc.hawaii.edu wrote:

}
}
}
}
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} Once upon a time I had to perform TEM on pineapples
} of different stages of
} ripeness, and I had problems that someone said was
} because glutaraldehyde
} doesn't fix well at low pH. (Nevermind the more
} pressing problem was the
} crystal of Si in each cell, and all that pineapple
} juice...) Now I have
} clients who want to fix bacteria that are being
} cultured at a pH of about
} 3.0. Do any of you have any suggestions for a
} fixation protocol? Cryo is
} not an option at this time.
}
} Mahalo,
} Tina
}
}
****************************************************************************
} * Tina (Weatherby) Carvalho *
} tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808)
} 956-6251 *
} * University of Hawaii at Manoa *
} http://www.pbrc.hawaii.edu/bemf*
}
****************************************************************************
}
}
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____________________________________________________________________________________
Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center.
http://autos.yahoo.com/green_center/

==============================Original Headers==============================
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15, 21 -- Subject: Re: [Microscopy] Fixing low pH extremophiles
15, 21 -- To: tina-at-pbrc.hawaii.edu
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From: mike.reedy-at-cellbio.duke.edu
Date: Thu, 21 Jun 2007 05:51:47 -0500
Subject: [Microscopy] Re: Fixing low pH extremophiles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Uranyl acetate may be worth trying, since 1% water solutions have a
pH around 3.8. Yet remarkable efficacy has been reported for UrAc
at pH 7.2 as a primary fixative for whole tissue structure and
immunolabeling by Fassel & Greaser 1997 (Microsc Res Tech 37:600-601)

and at unadjusted pH 3.8 as an agent for time-resolved fixation of
fleeting intermediates during rapid structural transitions when used
for negative staining by Zhao & Craig 2003 (J Struct Biol 141:43-52;
J Mol Biol 327:145-58).

-mike reedy-
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
Dept. Cell Biology, Box 3011 (for U.S. Mail)
458 Alex Sands Bldg, Research Dr (courier)
Durham, NC 27710

Office 919-668-2534
Lab 919-684-5674
Fax 919-681-9929
mike.reedy-at-cellbio.duke.edu
http://note.cellbio.duke.edu/Faculty/Research/Reedy.html

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From: bozhilov-at-ucr.edu
Date: Thu, 21 Jun 2007 10:34:44 -0500
Subject: [Microscopy] Re: 200KeV TEM users Opinions, please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,

What you are pointing out is true in principle and in theory and it
could affect seriously only dedicated HR TEM instruments.

I have experience with a Philips/FEI EM, CM and Tecnai TEMs. As an
example I will point out to oue CM300 which for the past over 10
years has been operated routinely between 100, 200 and 300 kV. This
machine has theoretical point resolution of 0.23 nm and we have never
encountered problems with obtaining the specs for resolution and
stability even when we had to switch between different voltages
within several hours time span.

If you have a FEG instrument with objective lenses with small
coefficient for spherical aberration then minor instabilities are
prone to cause considerable consequences but on a W or LaB6
instrument with relatively large gap between the objective polepieces
the problems are negligible.

In essence what I want to says that if one needs to operate an
instrument at the highest possible resolution and stability then
operating at the maximum voltage is probably what should be expected
but for a machine designed and dedicated for universal application
there are no serious problems with using range of voltages
specifically with the FEI twin lens instrument which I have extended
experience. I suspect that this would be true for JEOL, Hitachi and
Zeiss but cannot confirm it from my own experience.

Krassimir.
===================================
Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

tel. 951 827 2998
fax 951 827 2489
===================================


On Jun 20, 2007, at 12:52 PM, Mardinly, John wrote:

} Richard, Krassimir;
} Please let me contribute one aspect to this thread that has not
} been considered: unless you purchase a TEM with constant power
} lenses, and I am not aware of any other than the FEI Titan, you
} will have a lengthy period of thermal instability following an
} accelerating voltage change because the lens currents change
} significantly. Dropping accelerating voltage can result in over-
} cooling the column, which can result in condensation, vacuum leaks
} and thermal drift, even if the water flow is adjusted. Raising the
} accelerating voltage after an extended period of low-voltage
} operation can result in a need to condition the accelerator to
} avoid HT instability. Those are some of the other reasons that TEMs
} generally run at their rated high voltage.
}
} John Mardinly
} Intel Corporation
}
} This comment does not represent an opinion of Intel Corporation.
}
} -----Original Message-----
} From: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu]
} Sent: Wednesday, June 20, 2007 8:57 AM
} To: Mardinly, John
} Subject: [Microscopy] 200KeV TEM users Opinions, please
}
}
}
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
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} ----------------------------------------------------------------------
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}
} Richard,
}
} Yes, there is no problem running at any kV one desires.
}
} Furthermore the quality of images at higher voltages is far superior
} due to the better coherence of the el. beam for the LaB6 cathode and
} decreased inelastic scattering compared to tungsten and low voltage.
}
} Also the need of lower voltage for improved contrast is questionable
} if the system is equipped with digital imaging capability. As long as
} resolution is there contrast can be improved quite significantly just
} by post-acquisition processing.
}
} Radiolysis is the main problem causing specimen damage at voltages
} less than 300 kV and this increases with decreasing accelerating
} voltage. That is another advantage to operate at higher voltages.
}
} The only drawback for having higher voltage instrument with LaB6 is
} the stricter vacuum requirements.
} For many life science applications this my cause some slowdown since
} pumping times will be longer during sample exchange. This can be
} resolved by obtaining multiple-grid specimen holders.
}
} I would even suggest if funding is not a problem to go for a 300 kV
} instrument, provided you expect to do substantial work in materials
} science and geology.
}
} Krassimir.
}
} ===================================
} Krassimir N. Bozhilov
} Central Facility for Advanced Microscopy and Microanalysis
} University of California
} Riverside, CA 92521
}
} tel. 951 827 2998
} fax 951 827 2489
} ===================================
}
}
} On Jun 20, 2007, at 5:27 AM, edelmare-at-Muohio.edu wrote:
}
} }
} }
} }
} } ---------------------------------------------------------------------
} } -
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/
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} } -
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} }
} } Krassimir:
} }
} } Then you have no problem running any KeV at any time in your TEM(s)?
} }
} }
} } On 19 Jun 2007 at 10:02, K.N. Bozhilov wrote:
} }
} } } In an universal applications environment the only good reason to buy
} } } 120kV vs 200kV TEM is lack of money.
} } }
} } } ===================================
} } } Krassimir N. Bozhilov
} } } Central Facility for Advanced Microscopy and Microanalysis
} } } University of California
} } } Riverside, CA 92521
} } }
} } } tel. 951 827 2998
} } } fax 951 827 2489
} } } ===================================
} } }
} } }
} } } On Jun 19, 2007, at 9:12 AM, edelmare-at-muohio.edu wrote:
} } }
} } } }
} } } }
} } } }
} } } } -------------------------------------------------------------------
} } } } -
} } } } --
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} } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
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} } } }
} } } } 200Kev scope users:
} } } }
} } } } O.k., we are trying to evaluate 120Kev TEM´s vs 200Kev TEM´s with
} } } } LaB6 guns. Our biggest concerns come in routine usability, and
} } } } I am
} } } } hoping to hear any comments on any of the following questions we
} } } } have.
} } } }
} } } } We handle a wide range of samples (Biological thin sections,
} } } } organic
} } } } macro-molecules, geologic particles, geologic thin sections,
} } } } crystals, thin films, nano-particles, organic and non-organic
} } } } materials) with a wide range of techniques (BF, DF, Diffraction,
} } } } EDS,
} } } } EELS, including lattice fringe imaging, STEM and tomography
} } } } secondarily). In balancing contrast, resolution, and beam
} } } } damage we
} } } } really expect to perform a majority of our imaging between 80KeV
} } } } and
} } } } 120KeV (or 150KeV). However, the option of higher voltages up to
} } } } 200KeV, for higher resolution imaging is something we are
} } } } considering. So we are looking at both 120 and 200 KeV scopes.
} } } } (And
} } } } yes, we will test as many samples on any scope as we can but a
} } } } couple
} } } } of days worth of playing with a handful of samples does not compare
} } } } to years worth of experience.)
} } } }
} } } } (1) It seems that most 200Kev scopes are set to 200Kev and left
} } } } there - presumably because it eases alignments, voltage stability,
} } } } etc. when pushing for ultimate resolution. Is this true? Yes, all
} } } } the manufacturers list voltage ranges the scope can be operated at,
} } } } but does anyone really use anything else? With the digitizing of
} } } } the
} } } } scope controls and storage of multiple alignment setting, alignment
} } } } at different voltages (theoretically) should be fairly straight
} } } } forward but is it really?
} } } }
} } } } (2) Is 200KeV worth having if the majority of the work does not
} } } } require 200KeV? Not having personally worked much at 200KeV, Does
} } } } loss of contrast and beam damage at 200KeV limit usability when
} } } } imaging other than atomic / lattice level?
} } } }
} } } } (3) If the majority of the scope usage is 80 - 140 KeV is the cost
} } } } of a 200KeV instrument worth it? In terms of initial cost, and
} } } } maintenance. (Even if the only option tops off at 120KeV). We
} } } } are
} } } } all aware of instances of: "If you can at all afford it get,
} } } } even if
} } } } you won´t use it regularly, because . . ." is this one of those?
} } } }
} } } } (4) A few years ago the list had a discussion of W vs. LaB6, vs.
} } } } FEG/Schottky for SEM´s and the consensus basically was that LaB6
} } } } was
} } } } not worth the cost for SEM. (Either FEG if affordable or tungsten
} } } } with better bells & whistles). Is LaB6 worth it in terms of TEM?
} } } }
} } } } (5) What more important issue have we neglected in our naivete?
} } } }
} } } } (Boy, wouldn´t a 150KeV Schottky-FEG be a nice compromise?)
} } } }
} } } } Thank you for any info you're willing to share!
} } } }
} } } }
} } } }
} } } } Richard E. Edelmann, Ph.D.
} } } } EXPO Editor, Microscopy and Microanalysis Supplement
} } } } Electron Microscopy Facility Director
} } } } 364 Pearson Hall
} } } } Miami University, Oxford, OH 45056
} } } } Ph: 513.529.5712 Fax: 513.529.4243
} } } } E-mail: edelmare-at-muohio.edu
} } } } http://www.emf.muohio.edu
} } } }
} } } }
} } } }
} } } } ==============================Original
} } } } Headers==============================
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} } }
} }
} }
} } Richard E. Edelmann, Ph.D.
} } Electron Microscopy Facility Director
} } 364 Pearson Hall
} } Miami University, Oxford, OH 45056
} } Ph: 513.529.5712 Fax: 513.529.4243
} } E-mail: edelmare-at-muohio.edu
} } http://www.emf.muohio.edu
} }
} } "RAM disk is NOT an installation procedure."
} }
} }
} }
} } ==============================Original
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From: hall-at-aecom.yu.edu
Date: Thu, 21 Jun 2007 11:26:48 -0500
Subject: [Microscopy] pH effects on fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tina's query has brought to mind a parallel question that we have
been pondering.

Is the exact pH of the fix buffer important in fixing standard
biological tissue (not extremophiles).

Over the years, I have followed protocols that used pH 7.6 for some
tissues (vertebrates and invertebrates), and pH 7.4 or pH 7.2 for
others. This was never done systematically, but mostly by
indirection. We have recently been suffering some poor results on
invertebrate tissues - and wondering if there is a perfect pH that
would yield best results. Is it conceivable that a small shift in pH
is critical? In particular we have been testing microwave protocols,
where access across the outer cuticle may be limiting entry/exit of
fluids into the intact animal.

Any suggestions? I have always much more about osmolarity rather than pH.

Thanks in advance,

DHH
--
David H. Hall, Ph.D.
Center for C. elegans Anatomy
Dominic P. Purpura Department of Neuroscience
Albert Einstein College of Medicine
1410 Pelham Parkway
Bronx, NY 10461

www.wormatlas.org
www.wormimage.org

phone 718 430-2195
fax 718 430-2514

==============================Original Headers==============================
8, 25 -- From hall-at-aecom.yu.edu Thu Jun 21 11:26:47 2007
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From: Elliott-at-arizona.edu
Date: Thu, 21 Jun 2007 12:01:31 -0500
Subject: [Microscopy] Re: pH effects on fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi David

I have not done this systematically, but my impression is that the
osmolarity matters and the pH is much less (if at all) important.
That said, I have never done microwave, so I am not sure if that
changes things.

David


_____________________

David Elliott Ph.D.
Assistant Professor - Department of Cell Biology and Anatomy
Director, Research Microscopy Core Service
University of Arizona College of Medicine
PO Box 245004
Tucson, AZ 85724

Voice: 520-626-7870
Fax: 520-626-2097


On Jun 21, 2007, at 9:29 AM, hall-at-aecom.yu.edu wrote:

}
}
}
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}
} Tina's query has brought to mind a parallel question that we have
} been pondering.
}
} Is the exact pH of the fix buffer important in fixing standard
} biological tissue (not extremophiles).
}
} Over the years, I have followed protocols that used pH 7.6 for some
} tissues (vertebrates and invertebrates), and pH 7.4 or pH 7.2 for
} others. This was never done systematically, but mostly by
} indirection. We have recently been suffering some poor results on
} invertebrate tissues - and wondering if there is a perfect pH that
} would yield best results. Is it conceivable that a small shift in pH
} is critical? In particular we have been testing microwave protocols,
} where access across the outer cuticle may be limiting entry/exit of
} fluids into the intact animal.
}
} Any suggestions? I have always much more about osmolarity rather
} than pH.
}
} Thanks in advance,
}
} DHH
} --
} David H. Hall, Ph.D.
} Center for C. elegans Anatomy
} Dominic P. Purpura Department of Neuroscience
} Albert Einstein College of Medicine
} 1410 Pelham Parkway
} Bronx, NY 10461
}
} www.wormatlas.org
} www.wormimage.org
}
} phone 718 430-2195
} fax 718 430-2514
}
} ==============================Original
} Headers==============================
} 8, 25 -- From hall-at-aecom.yu.edu Thu Jun 21 11:26:47 2007
} 8, 25 -- Received: from mx1.aecom.yu.edu (mx1.aecom.yu.edu
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==============================Original Headers==============================
11, 22 -- From Elliott-at-arizona.edu Thu Jun 21 12:01:30 2007
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11, 22 -- Subject: Re: [Microscopy] pH effects on fixation
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From: nizets2-at-yahoo.com
Date: Thu, 21 Jun 2007 14:59:04 -0500
Subject: [Microscopy] pH effects on fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi!

There is an optimum for everything, so there is no
doubt that an optimal pH exists. The question is
whether it is worth to spend much time to find it when
you can be happy with a "routine" protocol.

--- Elliott-at-arizona.edu wrote:

}
}
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}
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} Microscopy Society of America
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}
} Hi David
}
} I have not done this systematically, but my
} impression is that the
} osmolarity matters and the pH is much less (if at
} all) important.
} That said, I have never done microwave, so I am not
} sure if that
} changes things.
}
} David
}
}
} _____________________
}
} David Elliott Ph.D.
} Assistant Professor - Department of Cell Biology and
} Anatomy
} Director, Research Microscopy Core Service
} University of Arizona College of Medicine
} PO Box 245004
} Tucson, AZ 85724
}
} Voice: 520-626-7870
} Fax: 520-626-2097
}
}
} On Jun 21, 2007, at 9:29 AM, hall-at-aecom.yu.edu
} wrote:
}
} }
} }
} }
} }
}
----------------------------------------------------------------------
}
} } ------
} } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/
} } MicroscopyListserver
} } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
----------------------------------------------------------------------
}
} } ------
} }
} } Tina's query has brought to mind a parallel
} question that we have
} } been pondering.
} }
} } Is the exact pH of the fix buffer important in
} fixing standard
} } biological tissue (not extremophiles).
} }
} } Over the years, I have followed protocols that
} used pH 7.6 for some
} } tissues (vertebrates and invertebrates), and pH
} 7.4 or pH 7.2 for
} } others. This was never done systematically, but
} mostly by
} } indirection. We have recently been suffering some
} poor results on
} } invertebrate tissues - and wondering if there is a
} perfect pH that
} } would yield best results. Is it conceivable that
} a small shift in pH
} } is critical? In particular we have been testing
} microwave protocols,
} } where access across the outer cuticle may be
} limiting entry/exit of
} } fluids into the intact animal.
} }
} } Any suggestions? I have always much more about
} osmolarity rather
} } than pH.
} }
} } Thanks in advance,
} }
} } DHH
} } --
} } David H. Hall, Ph.D.
} } Center for C. elegans Anatomy
} } Dominic P. Purpura Department of Neuroscience
} } Albert Einstein College of Medicine
} } 1410 Pelham Parkway
} } Bronx, NY 10461
} }
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} }
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7, 21 -- From nizets2-at-yahoo.com Thu Jun 21 14:59:04 2007
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From: dac-at-research.umass.edu
Date: Thu, 21 Jun 2007 15:36:35 -0500
Subject: [Microscopy] Re: pH effects on fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I think that generally the pH should be "neutral" to keep proteins
soluble, but that is a fairly broad range. The buffering capacity of the
fixative may be important to keep the pH "good" in use; in plants,
penetration of the fixatives can be slow and the vacuolar sap is acidic
so if the membrane becomes permeablized before enough buffering is
present the environment for the actual fixation in internal cells may go
acidic during the critical minutes of early fixation. I think that some
of the high pH fixes for plants (} pH 8) may be trying to hedge a bit,
starting on the high edge so the result is neutral?

I wrote to Tina a couple of days ago about the extremophiles low pH and
admitted to having to fix beef samples at pH 3.5 and I was surprised
that the the tissue (if we can generosly call it that..please .. for my
self esteem...) gelled very quickly at pH 3.5. Here it is important to
have the pH low during fixation because the "ex-tissue" does wild things
structurally and changing the pH to neutral could well alter the
structure before it fixes. However cells growing -at- pH3.5 may well have
neutral cytoplasm - maybe someone knows.

In the early 70's, a professor I worked for was excitedly showing my
glutaraldehyde-fixed, plastic-embedded material to his colleague, a
famous tropical botanist. After the tropical botanist was allowed some
period of study, he was asked what he thought. He thoughtfully replied,
"It is all very nice, but the cytoplasm obscures all the detail".

So, optimum is relative to the need and end result?

Dale Callaham
The University of Massachusetts -at- Amherst

nizets2-at-yahoo.com wrote:
} ----------------------------------------------------------------------------
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}
} Hi!
}
} There is an optimum for everything, so there is no
} doubt that an optimal pH exists. The question is
} whether it is worth to spend much time to find it when
} you can be happy with a "routine" protocol.
}
} --- Elliott-at-arizona.edu wrote:
}
} }
} }
} }
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} ----------------------------------------------------------------------------
} } Hi David
} }
} } I have not done this systematically, but my
} } impression is that the
} } osmolarity matters and the pH is much less (if at
} } all) important.
} } That said, I have never done microwave, so I am not
} } sure if that
} } changes things.
} }
} } David
} }
} }
} } _____________________
} }
} } David Elliott Ph.D.
} } Assistant Professor - Department of Cell Biology and
} } Anatomy
} } Director, Research Microscopy Core Service
} } University of Arizona College of Medicine
} } PO Box 245004
} } Tucson, AZ 85724
} }
} } Voice: 520-626-7870
} } Fax: 520-626-2097
} }
} }
} } On Jun 21, 2007, at 9:29 AM, hall-at-aecom.yu.edu
} } wrote:
} }
} } }
} } }
} } }
} ----------------------------------------------------------------------
} } } ------
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} } }
} } } Tina's query has brought to mind a parallel
} } question that we have
} } } been pondering.
} } }
} } } Is the exact pH of the fix buffer important in
} } fixing standard
} } } biological tissue (not extremophiles).
} } }
} } } Over the years, I have followed protocols that
} } used pH 7.6 for some
} } } tissues (vertebrates and invertebrates), and pH
} } 7.4 or pH 7.2 for
} } } others. This was never done systematically, but
} } mostly by
} } } indirection. We have recently been suffering some
} } poor results on
} } } invertebrate tissues - and wondering if there is a
} } perfect pH that
} } } would yield best results. Is it conceivable that
} } a small shift in pH
} } } is critical? In particular we have been testing
} } microwave protocols,
} } } where access across the outer cuticle may be
} } limiting entry/exit of
} } } fluids into the intact animal.
} } }
} } } Any suggestions? I have always much more about
} } osmolarity rather
} } } than pH.
} } }
} } } Thanks in advance,
} } }
} } } DHH
} } } --
} } } David H. Hall, Ph.D.
} } } Center for C. elegans Anatomy
} } } Dominic P. Purpura Department of Neuroscience
} } } Albert Einstein College of Medicine
} } } 1410 Pelham Parkway
} } } Bronx, NY 10461
} } }
} } } www.wormatlas.org
} } } www.wormimage.org
} } }
} } } phone 718 430-2195
} } } fax 718 430-2514
} } }
} } } ==============================Original
} } } Headers==============================
} } } 8, 25 -- From hall-at-aecom.yu.edu Thu Jun 21
} } 11:26:47 2007
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} } 11, 22 -- From Elliott-at-arizona.edu Thu Jun 21
} } 12:01:30 2007
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} ==============================Original Headers==============================
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} 7, 21 -- Subject: Re: [Microscopy] Re: pH effects on fixation
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==============================Original Headers==============================
6, 22 -- From dac-at-research.umass.edu Thu Jun 21 15:36:35 2007
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From: toli-at-hillweb.com
Date: Thu, 21 Jun 2007 19:03:19 -0500
Subject: [Microscopy] microscopes avaliable

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are upgrading our microscopes and have some surplus


1)Jeol JSM-35C
Working SEM, has been used as digital scope with A/D card (not included).
Includes Link Analytical backscattering detector.

2)Zeiss EM-10
Working TEM, fantastic resolution, includes Scion digital camera

3)ISI-40 SEM, working condition

4)Jeol 100-S TEM, lots of work done to restore machine, but has not been
turned
on recently

--


¦ Anatoli Oleynik, Research Associate - BioWarn, LLC.
¦ 964 East St. Suite 106a - Pittsboro, NC 27312
¦ O: 919.542.7410 | M: 919.260.1911 | F: 919.542.0268
¦ mobile email/MSN-M: cell.toli-at-hotmail.com
¦ www.biowarnllc.com


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From: c.goodsir-at-vla.defra.gsi.gov.uk
Date: Thu, 21 Jun 2007 20:22:42 -0500
Subject: [Microscopy] viaWWW: Non-specific labelling of myelin in epoxy resin embedded

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Email: c.goodsir-at-vla.defra.gsi.gov.uk
Name: Caroline Goodsir

Organization: Veterinary Laboratory Agency-Lasswade

Title-Subject: [Filtered] electron microscopy immunohistochemistry

Question: Non-specific labelling of myelin in epoxy resin embedded brain tissue!!!!!
Post-embedding immunocytochemistry of semi-thin (one micron thick) animal brain sections using ABC Technique, results in very high non-specific labelling of myelin. I have tried applying every blocking reagent I know with no success.

Can anyone solve this problem???????

Thankyou
Caroline Goodsir

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8, 12 -- brain tissue!!!!!
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From: toli-at-hillweb.com
Date: Thu, 21 Jun 2007 20:25:20 -0500
Subject: [Microscopy] viaWWW: Microscopes available

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Email: toli-at-hillweb.com
Name: Anatoli Oleynik

Organization: BioWarn

Title-Subject: [Filtered] Anyone have a good home?

Question: Electron microscopes available:
{br}
1)Jeol JSM-35C
Working SEM, has been used as digital scope with A/D card (not included).
Includes Link Analytical backscattering detector.
{br}
2)Zeiss EM-10
Working TEM, fantastic resolution, includes Scion digital camera
{br}
3)ISI-40 SEM, working condition
{br}
4)Jeol 100-S TEM, lots of work done to restore machine, but has not been turned
on recently
{br}

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From: edelmare-at-muohio.edu
Date: Fri, 22 Jun 2007 12:54:31 -0500
Subject: [Microscopy] Identify DNA in SEM

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Looking for a generic method for labeling DNA such that it shows up
in the SEM. Something genric like DAPI for LM rather than an insitu
hybridization with a gold label.

Any suggestions?

Some old Silver staining technique?

Thanks
Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


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From: paul_hazelton-at-umanitoba.ca
Date: Fri, 22 Jun 2007 13:06:01 -0500
Subject: [Microscopy] Re: Identify DNA in SEM

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Richard

How about an anti-DNA antibody? Been a while since I've used one, that
was for immunoblots, not EM. Most immunological suppliers can sell you
one. You can then detect with a gold labelled secondary antibody.

Paul


Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926


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From: mnesta-at-ebsciences.com
Date: Fri, 22 Jun 2007 14:14:01 -0500
Subject: [Microscopy] Unsubscribe

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From: nizets2-at-yahoo.com
Date: Fri, 22 Jun 2007 14:21:05 -0500
Subject: [Microscopy] Re: Identify DNA in SEM

Contents Retrieved from Microscopy Listserver Archives
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It really depends on the context.

If it is possible, just one crazy idea: label your DNA
with BrdU and detect Br by EDX. In situ, you can also
use BrdU with the Tdt technique. BrdU can also be
detected with very good specific antibodies.

Stephane

--- edelmare-at-muohio.edu wrote:

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} Looking for a generic method for labeling DNA such
} that it shows up
} in the SEM. Something genric like DAPI for LM
} rather than an insitu
} hybridization with a gold label.
}
} Any suggestions?
}
} Some old Silver staining technique?
}
} Thanks
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Director
} 364 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}
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} Headers==============================
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From: beth-at-plantbio.uga.edu
Date: Fri, 22 Jun 2007 16:29:13 -0500
Subject: [Microscopy] Zeiss TEMs

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I have a friend looking for a Zeiss 109, 900 or 902 TEM.
If anyone is selling or giving away their old scope please let me know.
I saw the posting about the EM 10 (a great scope by the way).

Thanks for any leads,
Beth

**********************************************************************
Beth Richardson
Electron Microscopy Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

http://www.plantbio.uga.edu/emlab/

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
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From: tchallman-at-case4n6.com
Date: Fri, 22 Jun 2007 20:28:52 -0500
Subject: [Microscopy] viaWWW: SEM Parts Available_Phillips S505

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Email: tchallman-at-case4n6.com
Name: T. Challman

Organization: CASE Forensics Corp

Title-Subject: [Filtered] SEM Parts Available_Phillips S505

Question: We have a Phillips S505 SEM if anyone needs parts with a Tracor Northern EDS system. Please contact me off-line if you're interested. The tool is currently in Denver, CO.

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From: BTWells-at-petrog.co.uk
Date: Sat, 23 Jun 2007 08:59:02 -0500
Subject: [Microscopy] viaWWW: Unanswered questions

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Email: BTWells-at-petrog.co.uk
Name: Barrie Wells

Organization: Conwy Valley Systems Limited

Title-Subject: [Filtered] Unanswered questions

Question: Is there a list of unanswered questions? I donít mean in life generally, just on this list.
Some questions generate a wave of correspondence, others apparently none. Two questions recently were of interest to me, in that I wanted to know the answer, not that I could offer one, and so I kept an eye open, but saw nothing. The two questions were:
Title-Subject: [Filtered] RE: Segmentation of particles in Gatan Digital Micrograph Software (20/06/2007)
and
[Microscopy] auto particle ident (30/04/2007).
Did the posters receive answers off-line, or are there no answers, or were the questions too trivial for the people on the list, or are they the type of question one would expect to pay a consultant to answer? I am intrigued, especially as listers seem to give of their time and knowledge so freely on so many topics.
Thanks for any insights,
Barrie


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From: zaluzec-at-microscopy.com
Date: Sat, 23 Jun 2007 09:07:02 -0500
Subject: [Microscopy] Administrivia: Unanswered questions

Contents Retrieved from Microscopy Listserver Archives
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Barrie, raises a valid query, and there is no such list of questions still
waiting for answers. At the same time I believe there is a great number
of off-line answers to questions that have been received, but are not
circulated.

I'll take this opportunity to remind all subscribers that when
you receive off-line answers to a question, it would be very
appropriate to collect all of these into a single message (removing
all respondant's names & affiliations) and then posting a SUMMARY
to the listserver. In this way everyone will benefit and the answers
which then also become a resource in the Listserver Archives.

There are a number of occassions where individuals do post summaries,
but I expect that this percentage is on the low side relative to the actual
number of responses.

Of course, if a respondant request you keep his/her reply private then by all
means you should honor that request.

Cheers,

Nestor
Your Friendly Neighborhood SysOp

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From: jennings.michael-at-gmail.com
Date: Mon, 25 Jun 2007 08:03:48 -0500
Subject: [Microscopy] viaWWW: Ruthenium Stains

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Email: jennings.michael-at-gmail.com
Name: Mike

Organization: university of Otago

Title-Subject: [Filtered] Ruthenium Stains

Question: Hello All!

I have a quick question about contrast using Ruthenium Red stain on cells for TEM.
I have some sections that have RR on them, and am wondering about the contrast for looking at microtubule structures compared to say Uranyl Acetate or OsO4?

Best Regards,

Mike



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From: rcmoretz-at-gmail.com
Date: Mon, 25 Jun 2007 13:47:14 -0500
Subject: [Microscopy] Re: viaWWW: Ruthenium Stains

Contents Retrieved from Microscopy Listserver Archives
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Michael:

Not quite information. Was the ruthenium red added as part of the
fixation protocol (in conjunction with the glut and OsO4) or is it an
en bloc stain or on the section? If it was in the initial fixative,
the penetration is very slight such that probably less than a micron
or so is stained (that is, on minced tissue). In intact tissues or
cultured cells, the RR is confined to the cell surface. There is a
whole literature on this in the 1960s and 1970s (and maybe into the
1980s--I don't remember) looking at staining with RR, alcian blue and
others. I do not know of techniques using RR for either en bloc or
thin section stains. Ruthenium tetroxide is a different matter--and
there is a separate literature on that as well. Try searching with
Google, or better, search through PubMed.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
Ridgefield, CT

July 4th--true independence day--is only 8 days away!!!!!!!!!! Then
I'm on the "codger side".


On 6/25/07, jennings.michael-at-gmail.com {jennings.michael-at-gmail.com} wrote:
}
}
}
} ----------------------------------------------------------------------------
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} ---------------------------------------------------------------------------
}
} Email: jennings.michael-at-gmail.com
} Name: Mike
}
} Organization: university of Otago
}
} Title-Subject: [Filtered] Ruthenium Stains
}
} Question: Hello All!
}
} I have a quick question about contrast using Ruthenium Red stain on cells for TEM.
} I have some sections that have RR on them, and am wondering about the contrast for looking at microtubule structures compared to say Uranyl Acetate or OsO4?
}
} Best Regards,
}
} Mike
}
}
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
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} 11, 11 -- Subject: viaWWW: Ruthenium Stains
} 11, 11 -- Content-Type: text/plain; charset="us-ascii"
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From: rcmoretz-at-gmail.com
Date: Mon, 25 Jun 2007 14:23:17 -0500
Subject: [Microscopy] Re: ruthenium red

Contents Retrieved from Microscopy Listserver Archives
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Ok. So I left the word "enough" out of the 1st sentence. I'm ready
to retire. Who cares, right? Then there were the many many many many
many "out of office" replies. Give me a break.

Roger Moretz

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From: bozzola-at-siu.edu
Date: Mon, 25 Jun 2007 14:29:36 -0500
Subject: [Microscopy] Re: Identify DNA in SEM

Contents Retrieved from Microscopy Listserver Archives
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This is not such an easy request since most generic EM stains will
disclose both DNA and RNA. In the SEM, however, if the RNA is
diffused and not concentrated (as the DNA in chromosomes), then UA
may suffice.

Ammoniacal silver has been used to disclose nuclei in tissues
examined in the SEM (Horiguchi, T, F. Sasaki, H. Takahama. 1984.
Stain Technology 59:143-48).

Although I have not used it, thallium ethylate is supposed to be
selective for "DNA-containing structures" (Moyne, G. 1973. Feulgen
derived techniques for electron microscopical cytochemistry of DNA.
J. Ultrastruc. Res. 45: 102-114). Thallium, of course, is toxic so
exercise precaution. The technique is very laborious and one has to
prepare the reagent using thallium metal in ethanol (10-20 days to
generate). Obviously, check the reference before jumping onto this
one. However, it is based on the Feulgen procedure which is rather
specific, at least on the LM level, for DNA.

Good luck.

JB

} Looking for a generic method for labeling DNA such that it shows up
} in the SEM. Something genric like DAPI for LM rather than an insitu
} hybridization with a gold label.
}
} Any suggestions?
}
} Some old Silver staining technique?
}
} Thanks
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Director
} 364 Pearson Hall
} Miami University, Oxford, OH 45056


--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

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From: drk-at-SHCC.org
Date: Mon, 25 Jun 2007 18:20:07 -0500
Subject: [Microscopy] Gatan Cold Stage for Philips TEM

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Hello Fellow Microscopists,

We have a Gatan Cold Stage with Control Unit Power Supply and cryo-transfer
station that we bought for our Philips 410 and barely used. We are
replacing the 410 with a new TEM and would like to find a home for this
cryostage. As far as I know it will fit the Philips 400 and CM series, as
well as some of the FEI TEMs. Can anyone suggest a mechanism for selling
such an item?

Thanks!

Doug

Douglas R. Keene
Assistant Investigator
Micro-Imaging Center
Shriners Hospital for Children
3102 S.W. Sam Jackson Park Road
Portland, Oregon 97239
503-221-3434
drk-at-shcc.org


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From: ssoleimani-at-sas.samsung.com
Date: Mon, 25 Jun 2007 18:36:48 -0500
Subject: [Microscopy] viaWWW: TEM Engineer Position Available

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Email: ssoleimani-at-sas.samsung.com
Name: Shelby Soleimani

Organization: Samsung Austin Semiconductor

Title-Subject: [Filtered] TEM Engineer Position Available

Question: This position will be dedicated to TEM operation and data reporting. The person will be engaged in root cause investigation on manufacturing excursion and on defect generation for fab yield improvement, and in driving to cut down analysis TAT.

Responsibilities include:
ïManage daily TEM Microscope imaging
ïPerform TEM imaging and summary write ups
ïOversee TEM Sample prep and provide feedback
ïWork with unit process and product technology engineers to resolve device and in line issues

Qualified candidates will possess:
ïMS or Ph.D. in EE, ChemE, Physics, or Material Science or equivalent
ïTEM microscope hands on operation
ïKnowledge of DRAM device technology and fabrication process
ïFLASH device knowledge
ïPermanent Residency of the United States is required.


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From: frah0010-at-umn.edu
Date: Mon, 25 Jun 2007 20:47:58 -0500
Subject: [Microscopy] Re: Particle Analysis

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Microscopists,

Barrie's recent post noted that there was little public reply to my
inquiry about automated particle analysis back in April, and I just
received another email asking what I had learned from my call for
opinions.

Therefore, due to popular demand, I'll summarize some of the replies
and what I learned here:

First of all, I should explain that my software experience in this
area is limited to NIH Image/ImageJ and an older version of
ImageSoft's analySIS. I've been satisfied with both for analyzing a
small number of images, but my collaborator was looking to analyze a
large number of images automatically after she set the parameters.

My collaborator is already using a piece of software to recognize/
analyze particles in her samples, but we were interested in exploring
other options. The program that she is using is called Colormod,
about which I know almost nothing -- I'll be seeing some of her
processed images soon, and I can offer an informed opinion then. Any
listers who have used Colormod, can you send me your impressions,
good or bad?

I received one message from a company that specializes in optical and
non-contact metrology. They sell several different packages: Nikon
Elements, Clemex, OSIS analysis (formerly SIS), and I Solutions from
IMT. Their company rep thought that the "I Solution" package would
probably suite the problem I described. These packages start at $900
and exceed $5000 for more advanced plug-ins. He thought the $900
package would suffice for manual interactive measurements and the
$3000 package would be ideal for automatic particle shape analysis
and measurement. He was nice enough to analyze one of our typical
images and prepare a sample report for our review.

Someone else pointed me toward Fovea Pro, a set of plugins used with
Photoshop. John Russ is the author of "The Image Processing
Handbook," and his son owns Reindeer Graphics, which makes Fovea Pro,
and works with his father to keep improving the software. This
researcher stated that "if you are familiar with John's expertise in
this field, there is noting that [Fovea Pro] cannot do, including
automated classifications." He argued the advantages of Fovea Pro
include integration with Photoshop, no proprietary restrictions in
image formats, and the ~$800 pricetag. We have not, though, had a
demo of Fovea Pro using a typical image from our study, as we had for
the I Solution software.

A few other emails gradually trickled in and offered either similar
or less suitable/practical options.

For now, at least, my collaborator is still working with Colormod,
but I am expecting to recommend some other program, like the various
packages mentioned by those who replied to my original post. I still
welcome other opinions regarding this topic and what software does or
does not work well for (largely) automated particle analysis.

Cheers,
Ellery

--------------------
Ellery E. Frahm
Research Fellow & Manager
Electron Microprobe Laboratory
University of Minnesota - Twin Cities
Department of Geology & Geophysics
Lab Website: http://probelab.geo.umn.edu
Personal Website: http://umn.edu/~frah0010


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From: christopher.parmenter-at-warwick.ac.uk
Date: Tue, 26 Jun 2007 05:04:41 -0500
Subject: [Microscopy] Explanation of CNT behaviour in TEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

I have enjoyed reading the discussion over the past few days and hope you
don't mind if I ask a question related to the whole 200 kV versus lower kV
instrument discussion. I recently had discussion with a colleague concerning
specimen damage in a TEM at 200 kV versus lower kV and told them that 200 kv
machines actually do less damage to samples due to faster moving electrons
and therefore lower interaction times (something someone echoed here on the
list). What we couldn't get to the bottom of was the reason that, when
investigating carbon nanotubes, the person in question said she saw more
damage in a 200 kV machine that at lower kV, suggesting there was less time
to image the tubes at higher kV. My question is this, is there something
particular to carbon nanotubes in the TEM, that makes their radiation damage
behaviour contrary to the established rules?

Many thanks

Chris

Dr Christopher DJ Parmenter
Fellow of the Royal Microscopal Society
Electron Microscopy and Imaging Suite
Biological Sciences
University of Warwick
02476574544
christopher.parmenter-at-warwick.ac.uk



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From: zaluzec-at-microscopy.com
Date: Tue, 26 Jun 2007 08:15:59 -0500
Subject: [Microscopy] Re: Explanation of CNT behaviour in TEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Chris

There are (at least) three mechanisms of specimen damage in the
EM. In order of their occurance with kV:

1.) Radiolysis - the energy of the electrons break the molecular bonds in
materials and disrupts the structure. This is the
principle effect in organic materials and what most
people were talking about previously. The cross section for this effect
decreases with increasing Energy. Hence the motivation to
increase the kV.

2.) Sputtering - the energy of the electron is sufficient to remove
an atom from the surface of the material (most frequently
the beam exit surface of the sample) . The cross-section
for this has a threshold and then increases with Energy.
Depending upon the cleanliness of your microscope you
may or maynot see this effect. Hydrocarbon contamination
does not sputter strongly, and thus acts like "glue" holding
the surface atoms of your real sample in place, so to speak.

3.) Displacement - the energy of the electron is sufficient to knock
an atom out of its location in a crystalline lattice inside
of your sample. This is the highest energy process, and
is the bane of materials scientists as they go to higher
voltage instruments to look at thicker samples, or when
high voltage is used for high resolution. This effect
creates vacancies, interstitials etc.. .which are usually
mobile. BTW, this is sometimes called Knock-on damage.

All of these effects are a function of atomic number, bond strength,
lattice strength, dose, dose rate and kinetic energy of the electron beam.
Each will manifest itself at different energies for different materials.

Because of all of these factors for each material there is an optimum
voltage to operate at , which is at the local minimum created by the
intersection of these 3 processes. The important point is that it is never
zero in a typical TEM environment.

In your carbon nanotubes you are likely seeing #3. The simple experiment
to do is, keeping the illumination conditions approximately constant, drop
the kV in your instrument and observe the nano-tubes. You will find a
voltage at which the damage as a function of time is minimized. My guess
is that this will be ~ 90-100 kV. Because of the threshold, and the fact that
it takes time for damage to accumulate, you can work at higher voltages
for short periods of time before defects accumulate sufficiently to disrupt a
given materials structure. Of course if your working with single walled
carbon nanotubes this won't be very long.


I'll digup a few papers on sputtering/displacement damage that colleagues and I worked
on a sometime ago on this and put it on the following WWW site later
this morning if your interested in reading abit more.

http://tpm.amc.anl.gov/Lectures

There should be references to the sputtering/displacement damage calculations
therein. For the radiolysis you will have to go to the life science EM literature, or
hopefully, one of our life science colleagues will post a message.

Nestor
Your Friendly Neighborhood SysOp



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From: jmkrupp-at-ucsc.edu
Date: Tue, 26 Jun 2007 13:23:01 -0500
Subject: [Microscopy] SEM of bones

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings:

I am looking for any advice/experience you might offer regarding the
preparation of bones for SEM.

I have a researcher who is doing a project on how bones fracture
following blunt force and other types of injury. She is also working
to understand if she can tell if the fracture happened pre or
postmortem. She is a forensic anthropologist who works for a local
sheriff's office and while it's not quite CSI, it's as close as I am
going to get.

Our first problem has come up with her test bones. She is using cow
bones and some of them appear to have a lot of fat or other 'stuff'
in them. Prior to coating for SEM, I put the bones in a VE for a
couple of days to be sure they were dry and coatable. Even after
several days in the VE, some of the samples still glisten and look
'wet' and juicy. Not ready to coat or put in my conventional SEM.

Looking for ideas about how to prepare these bones for SEM, alcohol
soak?, detergent soak?, something else?

I am not a boneologist and would appreciate any ideas.

Thanks

Jon
--

Jonathan Krupp
Microscopy & Imaging Lab
C230 Earth & Marine Science
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-ucsc.edu

I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money
for the San Francisco AIDS Foundation. Visit
http://www.aidslifecycle.org for more information about the ride or
http://www.aidslifecycle.org/5482 to make a donation.

==============================Original Headers==============================
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From: oshel1pe-at-cmich.edu
Date: Tue, 26 Jun 2007 13:55:37 -0500
Subject: [Microscopy] Re: SEM of bones

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jon,

I've done horse bone (cannon bone) looking for micro-fractures, and
those were simply air-dried, no other treatment (except metal
coating). This worked fine for a FESEM, but I suspect the pieces were
much smaller than the ones you're dealing with.
If you have fat on or in the bones, I'd try soaking them in
chloroform for 30 minutes X 3 to a day or three, depending on the
bone size, then air-dry. Chloroform works a treat to de-fat cheese
(and "cheese"), and should work well for bone. It dries off nicely,
once you get to the final change when there's only chloroform left.
Using detergent would leave behind a detergent residue that would
then need to be cleaned off, and EtOH or MeOH wouldn't get all the
fat. t-BuOH might, though -- I haven't tried that.

Phil

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From: gary.m.brown-at-exxonmobil.com
Date: Tue, 26 Jun 2007 14:09:05 -0500
Subject: [Microscopy] Re: SEM of bones

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jon,

I bet that this has been studied before and that the information is
available in the open literature or pathology texts. A quick (several
minute) search on Google gave me plenty of sites concerning osteology and
osteologists.

I suspect that her study would best be done on human bones. A good contact
for source of human bones may be the local morgue or medical school. Your
colleague's work in forensics at a sheriff's office may lend credence to
her request for human bones.

I wonder if an osteologist would think being called a boneologist is very
humerus?

Regards,


Disclaimer: The comments and opinions given above are those of the author
alone and do not represent any position of ExxonMobil Chemical Company.

Gary M. Brown
Microscopy Specialist
ExxonMobil Chemical Company
Baytown Technology & Engineering - West
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: 281 834 2387
fax: 281 834 2395
e-mail: Gary.M.Brown-at-ExxonMobil.com

"Happiness equals reality minus expectations." Tom Magliozzi





jmkrupp-at-ucsc.e
du
To
gary.m.brown-at-exxonmobil.com
06/26/07 01:25 cc
PM
Subject
[Microscopy] SEM of bones
Please respond
to
jmkrupp-at-ucsc.e
du











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Greetings:

I am looking for any advice/experience you might offer regarding the
preparation of bones for SEM.

I have a researcher who is doing a project on how bones fracture
following blunt force and other types of injury. She is also working
to understand if she can tell if the fracture happened pre or
postmortem. She is a forensic anthropologist who works for a local
sheriff's office and while it's not quite CSI, it's as close as I am
going to get.

Our first problem has come up with her test bones. She is using cow
bones and some of them appear to have a lot of fat or other 'stuff'
in them. Prior to coating for SEM, I put the bones in a VE for a
couple of days to be sure they were dry and coatable. Even after
several days in the VE, some of the samples still glisten and look
'wet' and juicy. Not ready to coat or put in my conventional SEM.

Looking for ideas about how to prepare these bones for SEM, alcohol
soak?, detergent soak?, something else?

I am not a boneologist and would appreciate any ideas.

Thanks

Jon
--

Jonathan Krupp
Microscopy & Imaging Lab
C230 Earth & Marine Science
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-ucsc.edu

I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money
for the San Francisco AIDS Foundation. Visit
http://www.aidslifecycle.org for more information about the ride or
http://www.aidslifecycle.org/5482 to make a donation.

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From: jvtaylo-at-emory.edu
Date: Tue, 26 Jun 2007 14:41:20 -0500
Subject: [Microscopy] hydrophilic carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear MSA, I have heard about using UV light to make carbon coated grids
hydrophilic. Can anyone give me some specifics and perhaps some references?
On or off list, as you wish.

Thank you very much, Jeannette

--
Jeannette Taylor, Technologist II
IM&MF
Cherry L. Emerson Hall, Room E106
Emory University
1515 Dickey Drive
Atlanta, Georgia 30322

Phone: 404-712-8674
FAX: 404-727-7760
jvtaylo-at-emory.edu
http://www.electronmicroscopy.emory.edu/


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From: dsherman-at-purdue.edu
Date: Tue, 26 Jun 2007 15:12:15 -0500
Subject: [Microscopy] SEM of bones

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Regarding the use of chloroform...

I once had a visitor from Kentucky Fried Chicken who wanted to look at fried
coatings in the SEM. Of course I said sure thinking that we would look at a
little bit and then get to eat the leftover chicken. No such luck! The
coatings arrived without the chicken and with lots of grease.

I did as Phil suggested and put bits of the coating into chloroform to
remove the frying grease and it worked great. Got some really interesting
images of regular vs. extra crispy...

Debby

Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907



} From: {oshel1pe-at-cmich.edu}
} Reply-To: {oshel1pe-at-cmich.edu}
} Date: Tue, 26 Jun 2007 13:57:42 -0500
} To: {dsherman-at-purdue.edu}
} Subject: [Microscopy] Re: SEM of bones
}
}
}
}
} ----------------------------------------------------------------------------
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}
} Jon,
}
} I've done horse bone (cannon bone) looking for micro-fractures, and
} those were simply air-dried, no other treatment (except metal
} coating). This worked fine for a FESEM, but I suspect the pieces were
} much smaller than the ones you're dealing with.
} If you have fat on or in the bones, I'd try soaking them in
} chloroform for 30 minutes X 3 to a day or three, depending on the
} bone size, then air-dry. Chloroform works a treat to de-fat cheese
} (and "cheese"), and should work well for bone. It dries off nicely,
} once you get to the final change when there's only chloroform left.
} Using detergent would leave behind a detergent residue that would
} then need to be cleaned off, and EtOH or MeOH wouldn't get all the
} fat. t-BuOH might, though -- I haven't tried that.
}
} Phil
}
} } ----------------------------------------------------------------------------
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} } ----------------------------------------------------------------------------
} }
} } Greetings:
} }
} } I am looking for any advice/experience you might offer regarding the
} } preparation of bones for SEM.
} }
} } I have a researcher who is doing a project on how bones fracture
} } following blunt force and other types of injury. She is also working
} } to understand if she can tell if the fracture happened pre or
} } postmortem. She is a forensic anthropologist who works for a local
} } sheriff's office and while it's not quite CSI, it's as close as I am
} } going to get.
} }
} } Our first problem has come up with her test bones. She is using cow
} } bones and some of them appear to have a lot of fat or other 'stuff'
} } in them. Prior to coating for SEM, I put the bones in a VE for a
} } couple of days to be sure they were dry and coatable. Even after
} } several days in the VE, some of the samples still glisten and look
} } 'wet' and juicy. Not ready to coat or put in my conventional SEM.
} }
} } Looking for ideas about how to prepare these bones for SEM, alcohol
} } soak?, detergent soak?, something else?
} }
} } I am not a boneologist and would appreciate any ideas.
} }
} } Thanks
} }
} } Jon
} } --
} }
} } Jonathan Krupp
} } Microscopy & Imaging Lab
} } C230 Earth & Marine Science
} } University of California
} } Santa Cruz, CA 95064
} } (831) 459-2477
} } jmkrupp-at-ucsc.edu
} }
} } I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money
} } for the San Francisco AIDS Foundation. Visit
} } http://www.aidslifecycle.org for more information about the ride or
} } http://www.aidslifecycle.org/5482 to make a donation.
} --
} Philip Oshel
} Microscopy Facility Supervisor
} Biology Department
} 024C Brooks Hall
} Central Michigan University
} Mt. Pleasant, MI 48859
}
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From: bozzola-at-siu.edu
Date: Tue, 26 Jun 2007 15:26:54 -0500
Subject: [Microscopy] Re: hydrophilic carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Germicidal lamps (short wavelength UV light) can help make
hydrophobic grids somewhat more hydrophilic. Basically, the UV
ionizes the air, generating reactive molecules (like ozone) that then
react with the grid surfaces (possibly cleaning them of undesirable
organics such as oils). Before we started using the (much better) AC
glow-discharge method to make grids hydrophilic, we did use this
method. Place the coated grids on a filter paper (coated side facing
the lamp) about 6-12 inches away from the lamp for 5 minutes. They
should be used in a couple days as the effect wears off.

As with any new procedure, you will need to make adjustments to the
distance and times since germicidal lamps vary in strength and they
do become less effective as they age. I would start by placing the
lamp 6 inches away from the grids and then withdrawing a grid every
minute for so (4,5,6,7,8,9 min) and examining a stained preparation
in the TEM looking for a relatively uniformly spread stain with a
minimal number of patches of stain. Protect your eyes (goggles) and
hands (gloves) from the UV and be aware that shiny (stainless steel)
sturfaces reflect UV towards you.

JB

} Dear MSA, I have heard about using UV light to make carbon coated grids
} hydrophilic. Can anyone give me some specifics and perhaps some references?
} On or off list, as you wish.
}
} Thank you very much, Jeannette
}
} --
} Jeannette Taylor, Technologist II
} IM&MF
} Cherry L. Emerson Hall, Room E106
} Emory University
} 1515 Dickey Drive
} Atlanta, Georgia 30322

--
+++++++++++++++++++++++++++++++++++++++++++++++++++++++

John J. Bozzola, Ph.D., Director
Integrated Microscopy & Graphics Expertise (IMAGE)
Southern Illinois University
750 Communications Drive - MC 4402
Carbondale, IL 62901
Telephone: 618-453-3730

+++++++++++++++++++++++++++++++++++++++++++++++++++++++

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From: bhfrazer_us-at-yahoo.com
Date: Tue, 26 Jun 2007 23:16:16 -0500
Subject: [Microscopy] viaWWW: WDS spectrometers

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Email: bhfrazer_us-at-yahoo.com
Name: Brad Frazer

Title-Subject: [Filtered] WDS spectrometers

Question: Hello everyone,

I'm working on a soft x-ray project and I'm in need of a spectrometer.

I was thinking that a WDS spectrometer might be a good fit and was wondering if someone could help me decide if this is a good idea.

I'll be working at the C and N energies and ideally, would like an energy resolution of 1 eV or better. I can live with anything less than 10 eV.

Also, I would really like to find a parallel detection system that would give me a spectrum between 250 and 650 eV.

So I guess my questions are:

1.) What are typical and/or achievable energy resolutions in the 250 - 600 eV energy range for WDS spectrometers?

2.) Is parallel detection an option?

3.) Does anyone have a used WDS detector for sale that would work in this energy range? (Any SEM platform is fine)

Thanks for the help!


Brad Frazer





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From: nizets2-at-yahoo.com
Date: Wed, 27 Jun 2007 03:40:05 -0500
Subject: [Microscopy] help: air contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues!

I left an aluminium stub with a carbon tab on a shelf
in the lab for 5 days, then I coated it shortly with
carbon and analyzed the specimen by EDX+SEM at 20keV.
I see all sorts of particles usually several µm big,
but most interestingly the majority of the particles I
see are composed of Si and Mg with a Si/Mg ratio of
1.5-1.6. No trace of anything else (SUTW window).
I wonder what that can be.
Can it be that the special glass used for the lab
glassware contain Mg?
Any idea?

Regards,

Stephane




____________________________________________________________________________________
Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games.
http://sims.yahoo.com/

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From: dsoren-at-umich.edu
Date: Wed, 27 Jun 2007 08:07:34 -0500
Subject: [Microscopy] TEM-storage of glut-fixed retina

Contents Retrieved from Microscopy Listserver Archives
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Dear listers,

We have an investigator who would like to know if he can store
glutaraldehyde-fixed mouse eye tissue while he collects samples from
different litters born over a time span of 1 week or more. The gene
that they are investigating affects membranes, and they are
interested in doing TEM imaging of the photo-receptors and other
membraneous components in the cells.

I know that standard TEM doctrine recommends processing the tissue
immediately for optimal preservation of the ultrastructure, but does
anyone out there have any experience with storing this kind of
tissue, after glut fixation, for a week or more before proceeding to
the osmium step? Also, many recommend storing the tissue in a
buffer rinse rather than in the glut. Can anyone tell me if and why
this is important?

As always, thanks so much for your suggestions,

Dotty Sorenson


Dorothy Sorenson
Microscopy and Image-analysis Laboratory
Department of Cell and Developmental Biology
University Of Michigan Medical School
A807 BSRB
109 Zina Pitcher Place
Ann Arbor, MI 48109-2200
(734)763-1170
FAX (734)763-1166



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From: lcgould-at-med.cornell.edu
Date: Wed, 27 Jun 2007 08:24:50 -0500
Subject: [Microscopy] Re: TEM-storage of glut-fixed retina

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Dear Dotty-
Oh, you just brought back some unpleasant memories. A number of
years ago, a post-doc here (who had been an EM tech before he went
back to grad school, and so should have known better) brought me 80
rat eyes that he had accumulated over the course of a few months and
stored in glut., in the 'frige. He had injected some sort of virus
sub-retinally and was looking for the result. Well, after telling
him (and his PI) that I had concerns about the quality of the
fixation and possible artifacts, I processed the lot over the course
of a couple of weeks (after } 2 months, I didn't think it would
matter). The structure was fine, BUT everything was rife with
"fixation pepper" and so nothing was publishable (see: Artifacts in
Biological Electron Microscopy, Richard FE Crang & Karen L
Klomparens, eds, Plenum Press, 1988). It looks a lot like fine Pb
ppt, and I had to take pictures of unstained sections to prove to the
post-doc and the PI that all of that gunk was NOT due to my staining
technique.
I think that storing the tissues for up to 1 week should be OK. I've
had to do that on occasion and haven't seen any striking problems.
If I have to store material before I can process it all the way, I
prefer to do that in the post-osmium buffer. I don't know if that's
just my personal voodoo, or if there is solid science backing it up.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Weill-Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

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From: jae5-at-lehigh.edu
Date: Wed, 27 Jun 2007 08:27:20 -0500
Subject: [Microscopy] Kit for electron optics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I wish to construct an electron-optical column similar, in general
terms, to a gun in a CRT. I recall, long ago, that there was a company
that sold kits for doing this. A Constructor Set or Mecano for
assembling prototype designs from components in the kit.

I can not locate this through Google. Does anyone know if the company
still exists? Or, indeed, if you have such a kit you no longer need,
would you be willing to part with it?

Thanks,
Alwyn Eades
--
...........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


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From: nizets2-at-yahoo.com
Date: Wed, 27 Jun 2007 08:48:56 -0500
Subject: [Microscopy] Re: help: air contamination...and the winner is...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tom McKee has found my mystery substance. This is
Talc, which clearly corresponds to the EDX analysis
and which I shortly used last week in the lab.

Philip: I left the stub in the air on purpose. I
wanted to verify if the particles in my samples could
come from air contamination.

Thanks to all those who activated their neurons to
help me.

Stephane

--- nizets2-at-yahoo.com wrote:

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} Dear colleagues!
}
} I left an aluminium stub with a carbon tab on a
} shelf
} in the lab for 5 days, then I coated it shortly with
} carbon and analyzed the specimen by EDX+SEM at
} 20keV.
} I see all sorts of particles usually several µm big,
} but most interestingly the majority of the particles
} I
} see are composed of Si and Mg with a Si/Mg ratio of
} 1.5-1.6. No trace of anything else (SUTW window).
} I wonder what that can be.
} Can it be that the special glass used for the lab
} glassware contain Mg?
} Any idea?
}
} Regards,
}
} Stephane
}
}
}
}
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From: mike.reedy-at-cellbio.duke.edu
Date: Wed, 27 Jun 2007 12:54:46 -0500
Subject: [Microscopy] Re: hydrophilic carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would suggest that you go to the following site,
http://www.jelight.com/uvo-ozone-cleaning.php. What you are doing is
generating activated oxygen species and ozone. That is taking away the
hydrocarbons and making the sample hydrophilic. Actually, the UVO process
uses two wavelengths, one to break the molecule bonds and the other to
create the activated oxygen species and ozone. It almost does the same
thing as plasma cleaning and has been used for cleaning surfaces for a long
time.


-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com

-----Original Message-----
X-from: jvtaylo-at-emory.edu [mailto:jvtaylo-at-emory.edu]
Sent: Tuesday, June 26, 2007 12:46 PM
To: Walck-at-SouthBayTech.com

Dear MSA, I have heard about using UV light to make carbon coated grids
hydrophilic. Can anyone give me some specifics and perhaps some references?
On or off list, as you wish.

Thank you very much, Jeannette

--
Jeannette Taylor, Technologist II
IM&MF
Cherry L. Emerson Hall, Room E106
Emory University
1515 Dickey Drive
Atlanta, Georgia 30322

Phone: 404-712-8674
FAX: 404-727-7760
jvtaylo-at-emory.edu
http://www.electronmicroscopy.emory.edu/


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From cynthia_damian003-at-yahoo.co.in Wed Jun 27 12:33:15 2007
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Jeannette,

20+ years positive experience with UV-treated carbon films in a
leading EM research group is summarized and capped by them in Burgess
et al, Journal of Structural Biology 147 (2004) 247-258. I will email
it to you offlist.

I gather it might be a bit tricky to find the exact UV light they
used, or to find and verify a US equivalent. But the effort sounds
well worth making.

You should also look on SPI's website for some experienced and
thoughtful commentary on various methods for preparing hydrophilic,
hydrophobic, plus- and minus-charged surfaces etc. I'm not veruy good
at navigating the SPI site, but one of those sites is at
http://www.2spi.com/catalog/grids/cusctgrd.html


-mike reedy-

At 2:45 PM -0500 6/26/07, jvtaylo-at-emory.edu wrote:
} ----------------------------------------------------------------------------
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--
-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
Dept. Cell Biology, Box 3011 (for U.S. Mail)
458 Alex Sands Bldg, Research Dr (courier)
Durham, NC 27710

Office 919-668-2534
Lab 919-684-5674
Fax 919-681-9929
mike.reedy-at-cellbio.duke.edu
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From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Thu, 28 Jun 2007 02:19:18 -0500
Subject: [Microscopy] Re: Kit for electron optics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Look at :

www.*kimball**physics*.com/eV_parts/eV_prod.htm

They sell electron and ion guns but somthing like you are looking for
too. I've never used them, but it seems to be interesting.


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



jae5-at-lehigh.edu a écrit :
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}
} I wish to construct an electron-optical column similar, in general
} terms, to a gun in a CRT. I recall, long ago, that there was a company
} that sold kits for doing this. A Constructor Set or Mecano for
} assembling prototype designs from components in the kit.
}
} I can not locate this through Google. Does anyone know if the company
} still exists? Or, indeed, if you have such a kit you no longer need,
} would you be willing to part with it?
}
} Thanks,
} Alwyn Eades
}

==============================Original Headers==============================
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From: nizets2-at-yahoo.com
Date: Thu, 28 Jun 2007 02:51:01 -0500
Subject: [Microscopy] hydrophilic carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Is it not the principle of plasma cleaners?
Could it be that this works to clean samples too?

Regards,

Stephane

--- bozzola-at-siu.edu wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
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}
} Germicidal lamps (short wavelength UV light) can
} help make
} hydrophobic grids somewhat more hydrophilic.
} Basically, the UV
} ionizes the air, generating reactive molecules (like
} ozone) that then
} react with the grid surfaces (possibly cleaning them
} of undesirable
} organics such as oils). Before we started using the
} (much better) AC
} glow-discharge method to make grids hydrophilic, we
} did use this
} method. Place the coated grids on a filter paper
} (coated side facing
} the lamp) about 6-12 inches away from the lamp for 5
} minutes. They
} should be used in a couple days as the effect wears
} off.
}
} As with any new procedure, you will need to make
} adjustments to the
} distance and times since germicidal lamps vary in
} strength and they
} do become less effective as they age. I would start
} by placing the
} lamp 6 inches away from the grids and then
} withdrawing a grid every
} minute for so (4,5,6,7,8,9 min) and examining a
} stained preparation
} in the TEM looking for a relatively uniformly spread
} stain with a
} minimal number of patches of stain. Protect your
} eyes (goggles) and
} hands (gloves) from the UV and be aware that shiny
} (stainless steel)
} sturfaces reflect UV towards you.
}
} JB
}
} } Dear MSA, I have heard about using UV light to make
} carbon coated grids
} } hydrophilic. Can anyone give me some specifics and
} perhaps some references?
} } On or off list, as you wish.
} }
} } Thank you very much, Jeannette
} }
} } --
} } Jeannette Taylor, Technologist II
} } IM&MF
} } Cherry L. Emerson Hall, Room E106
} } Emory University
} } 1515 Dickey Drive
} } Atlanta, Georgia 30322
}
} --
}
+++++++++++++++++++++++++++++++++++++++++++++++++++++++
}
} John J. Bozzola, Ph.D., Director
} Integrated Microscopy & Graphics Expertise (IMAGE)
} Southern Illinois University
} 750 Communications Drive - MC 4402
} Carbondale, IL 62901
} Telephone: 618-453-3730
}
}
+++++++++++++++++++++++++++++++++++++++++++++++++++++++
}
} ==============================Original
} Headers==============================
} 7, 19 -- From bozzola-at-siu.edu Tue Jun 26 15:26:53
} 2007
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} (abbmta2.siu.edu [131.230.254.206])
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} 7, 19 -- Date: Tue, 26 Jun 2007 15:26:50 -0500
} 7, 19 -- To: Microscopy-at-msa.microscopy.com
} 7, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
} 7, 19 -- Subject: Re: [Microscopy] hydrophilic
} carbon film
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==============================Original Headers==============================
8, 20 -- From nizets2-at-yahoo.com Thu Jun 28 02:51:01 2007
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8, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com}
8, 20 -- Subject: Re: [Microscopy] Re: hydrophilic carbon film
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From: nizets2-at-yahoo.com
Date: Thu, 28 Jun 2007 02:58:01 -0500
Subject: [Microscopy] thin sections in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all!

I discovered "demo" examples in our SEM and one
example shows a TEM section with cells. The picture is
very good (of course the demo example are rarely made
of bad samples), very similar to what one can see by
TEM. I wonder how it is possible to obtain good images
of TEM section in SEM.
I guess that it is only possible using BSE mode.
Does anyone have experience with this? I wondered what
would be the optimal thickness, resin, preparation.
Are there special contrasting needs?

Best regards,

Stephane



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==============================Original Headers==============================
6, 19 -- From nizets2-at-yahoo.com Thu Jun 28 02:58:01 2007
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6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com}
6, 19 -- Subject: thin sections in SEM
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From: Michael.Fay-at-nottingham.ac.uk
Date: Thu, 28 Jun 2007 03:12:27 -0500
Subject: [Microscopy] hydrophilic carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You can use a plasma cleaner to do this, although normally you have to
be careful you don't clean for longer than a few seconds if you're using
grids with carbon films. However, with some plasma cleaner models there
are holders available to buy that will decrease the rate, making the
process more controllable.

Dr Mike Fay
Nottingham Nanotechnology and Nanoscience Centre


-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: 28 June 2007 08:55
To: michael.fay-at-nottingham.ac.uk

Is it not the principle of plasma cleaners?
Could it be that this works to clean samples too?

Regards,

Stephane

--- bozzola-at-siu.edu wrote:

}
}
}
}
------------------------------------------------------------------------
----
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
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----
}
} Germicidal lamps (short wavelength UV light) can
} help make
} hydrophobic grids somewhat more hydrophilic.
} Basically, the UV
} ionizes the air, generating reactive molecules (like
} ozone) that then
} react with the grid surfaces (possibly cleaning them
} of undesirable
} organics such as oils). Before we started using the
} (much better) AC
} glow-discharge method to make grids hydrophilic, we
} did use this
} method. Place the coated grids on a filter paper
} (coated side facing
} the lamp) about 6-12 inches away from the lamp for 5
} minutes. They
} should be used in a couple days as the effect wears
} off.
}
} As with any new procedure, you will need to make
} adjustments to the
} distance and times since germicidal lamps vary in
} strength and they
} do become less effective as they age. I would start
} by placing the
} lamp 6 inches away from the grids and then
} withdrawing a grid every
} minute for so (4,5,6,7,8,9 min) and examining a
} stained preparation
} in the TEM looking for a relatively uniformly spread
} stain with a
} minimal number of patches of stain. Protect your
} eyes (goggles) and
} hands (gloves) from the UV and be aware that shiny
} (stainless steel)
} sturfaces reflect UV towards you.
}
} JB
}
} } Dear MSA, I have heard about using UV light to make
} carbon coated grids
} } hydrophilic. Can anyone give me some specifics and
} perhaps some references?
} } On or off list, as you wish.
} }
} } Thank you very much, Jeannette
} }
} } --
} } Jeannette Taylor, Technologist II
} } IM&MF
} } Cherry L. Emerson Hall, Room E106
} } Emory University
} } 1515 Dickey Drive
} } Atlanta, Georgia 30322
}
} --
}
+++++++++++++++++++++++++++++++++++++++++++++++++++++++
}
} John J. Bozzola, Ph.D., Director
} Integrated Microscopy & Graphics Expertise (IMAGE)
} Southern Illinois University
} 750 Communications Drive - MC 4402
} Carbondale, IL 62901
} Telephone: 618-453-3730
}
}
+++++++++++++++++++++++++++++++++++++++++++++++++++++++
}
} ==============================Original
} Headers==============================
} 7, 19 -- From bozzola-at-siu.edu Tue Jun 26 15:26:53
} 2007
} 7, 19 -- Received: from abbmta2.siu.edu
} (abbmta2.siu.edu [131.230.254.206])
} 7, 19 -- by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with ESMTP id
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} 26 Jun 2007 15:26:53 -0500
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} 7, 19 -- Date: Tue, 26 Jun 2007 15:26:50 -0500
} 7, 19 -- To: Microscopy-at-msa.microscopy.com
} 7, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
} 7, 19 -- Subject: Re: [Microscopy] hydrophilic
} carbon film
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8, 20 -- Subject: Re: [Microscopy] Re: hydrophilic carbon film
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From: dsherman-at-purdue.edu
Date: Thu, 28 Jun 2007 07:27:13 -0500
Subject: [Microscopy] Re: thin sections in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stephane,

You can get very good SEM images from thin sections using a STEM detector.
This is great for unstained material and gives very good contrast. Thus it
is also good for ICC-labeled material where you have low contrast due to the
absence of osmium during fixation.

Debby

Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907



} From: {nizets2-at-yahoo.com}
} Reply-To: {nizets2-at-yahoo.com}
} Date: Thu, 28 Jun 2007 02:59:46 -0500
} To: {dsherman-at-purdue.edu}
} Subject: [Microscopy] thin sections in SEM
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi all!
}
} I discovered "demo" examples in our SEM and one
} example shows a TEM section with cells. The picture is
} very good (of course the demo example are rarely made
} of bad samples), very similar to what one can see by
} TEM. I wonder how it is possible to obtain good images
} of TEM section in SEM.
} I guess that it is only possible using BSE mode.
} Does anyone have experience with this? I wondered what
} would be the optimal thickness, resin, preparation.
} Are there special contrasting needs?
}
} Best regards,
}
} Stephane
}
}
}
} ______________________________________________________________________________
} ______
} Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail,
} news, photos & more.
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From: madelman-at-usuhs.mil
Date: Thu, 28 Jun 2007 07:40:50 -0500
Subject: [Microscopy] Seeking comment on "ProScope HR"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Is anyone familiar with this simple digital microscope/camera? We
wanted to evaluate it for possible use in our teaching (histology/
cell biology/etc.) labs, but the company is "unable" to lend it to us
on a demo basis. The Website link is

http://www.tedpella.com/mscope_html/ProScope.htm

Thanks in advance for any info you can provide. MRA

Mark R. Adelman, Ph.D.
Associate Professor of Anatomy, Physiology & Genetics
Uniformed Services University of the Health Sciences
4301 Jones Bridge Road
Bethesda, MD 20814-4799
USA
Phone: 301-295-3208
FAX: 301-295-1786
Email: madelman-at-usuhs.mil
Alternative work Email: adelman-at-educationalassistance.org
Website: http://www.educationalassistance.org/
Home Email: mra-at-educationalassistance.org


==============================Original Headers==============================
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5, 17 -- From: Mark Adelman {madelman-at-usuhs.mil}
5, 17 -- Subject: Seeking comment on "ProScope HR"
5, 17 -- Date: Thu, 28 Jun 2007 08:41:08 -0400
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5, 17 -- X-Mailer: Apple Mail (2.752.2)
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From: zhouda-at-umsl.edu
Date: Thu, 28 Jun 2007 08:07:37 -0500
Subject: [Microscopy] viaWWW: Ultramicrotome

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Email: zhouda-at-umsl.edu
Name: Dan Zhou

Organization: University of Missouri - St. Louis

Title-Subject: [Filtered] Ultramicrotome

Question: Dear All,

Our Electron Image and Spectroscopy Technique Lab is looking for an used ultramicrotome to cut TEM thin sections or to prepare the surface for SEM observation.

Please let me know If anyone is selling or giving away an used ultramicrotome.

Thank you very much for any leads,

Dan

Laboratory Manager, Center for Nanoscience
William L. Clay Building
University of Missouri-St. Louis
One University Boulevard
St. Louis, Missouri 63121

Phone: 314-516-4627
Fax: 314-516-4628
E-mail: zhouda-at-umsl.edu


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==============================Original Headers==============================
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From: emily.smith-at-nottingham.ac.uk
Date: Thu, 28 Jun 2007 08:07:58 -0500
Subject: [Microscopy] viaWWW: Twin jet electropolisher

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Email: emily.smith-at-nottingham.ac.uk
Name: Emily Smith

Organization: University of Nottingham, School of Chemistry

Title-Subject: [Filtered] Twin jet electropolisher

Question: Hi
I received an enquiry from a colleague for an electropolisher for TEM sample prep. Ideally he would like to buy secondhand as the new ones are out of the range of the budget.
Has anyone got one available? or know of a source?
Alternatively is there one in the North Oxfordshire area that could be utilised by my colleague for his prep?
(I'm in Nottingham - he's down South!)
Many thanks for any help
Emily

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==============================Original Headers==============================
6, 11 -- From zaluzec-at-microscopy.com Thu Jun 28 08:07:57 2007
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From: bfoster-at-mme1.com
Date: Thu, 28 Jun 2007 08:35:52 -0500
Subject: [Microscopy] Re: Seeking comment on "ProScope HR"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Mark

I seem to have seen this scope before ... it might be one of the Scalar line. It seemed to work OK, but I would really like to see it in action. I would suggest that you ask Pella if you can have it on 5 day trial, against a provisional purchase order. That way, no one loses. If you like it, you just pay for it. If you don't you return it, but Pella has the security of knowing that they will get payment.

Hope this was helpful,

Best regards,
Barbara Foster, President

We're moving July 1!
Microscopy/Microscopy Education
7101 Royal Glen Trail, Suite A
McKinney TX 75070
P: (972)924-5310
W: www.MicroscopyEducation.com


MME is now scheduling customized, on-site courses through September. Call us today for details.

P. S.
Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Just call us here in the MME office for details.



At 07:50 AM 6/28/2007, madelman-at-usuhs.mil wrote:



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==============================Original Headers==============================
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16, 18 -- From: Barbara Foster {bfoster-at-mme1.com}
16, 18 -- Subject: Re: [Microscopy] Seeking comment on "ProScope HR"
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From: wangjinfengumsl-at-hotmail.com
Date: Thu, 28 Jun 2007 08:39:45 -0500
Subject: [Microscopy] viaWWW: photoluminescence spectra for solid state sample

Contents Retrieved from Microscopy Listserver Archives
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Email: wangjinfengumsl-at-hotmail.com
Name: Jinfeng Wang

Organization: University of Missouri

Title-Subject: [Filtered] photoluminescence spectra for solid state sample

Question: Dear All,

Does anyone have experience to do photoluminescence for solid state sample? Should I put the sample into solution first?

Thanks advance for your information.

Jinfeng Wang

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==============================Original Headers==============================
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9, 11 -- From: wangjinfengumsl-at-hotmail.com (by way of MicroscopyListserver)
9, 11 -- Subject: viaWWW: photoluminescence spectra for solid state sample
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From: DusevichV-at-umkc.edu
Date: Thu, 28 Jun 2007 08:43:09 -0500
Subject: [Microscopy] hydrophilic carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I use my old Desk-1 sputter coater in mode "Etch" with very good
results. 5 milliamperes for 15 seconds.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: Michael.Fay-at-nottingham.ac.uk
} [mailto:Michael.Fay-at-nottingham.ac.uk]
} Sent: Thursday, June 28, 2007 3:13 AM
} To: Dusevich, Vladimir
} Subject: [Microscopy] RE: hydrophilic carbon film
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
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}
} You can use a plasma cleaner to do this, although normally
} you have to be careful you don't clean for longer than a few
} seconds if you're using grids with carbon films. However,
} with some plasma cleaner models there are holders available
} to buy that will decrease the rate, making the process more
} controllable.
}
} Dr Mike Fay
} Nottingham Nanotechnology and Nanoscience Centre
}
}
} -----Original Message-----
} X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
} Sent: 28 June 2007 08:55
} To: michael.fay-at-nottingham.ac.uk
} Subject: [Microscopy] hydrophilic carbon film
}
}
}
}
} --------------------------------------------------------------
} ----------
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ----------
} ----
}
} Is it not the principle of plasma cleaners?
} Could it be that this works to clean samples too?
}
} Regards,
}
} Stephane
}
} --- bozzola-at-siu.edu wrote:
}
} }
} }
} }
} }
} --------------------------------------------------------------
} ----------
} ----
} } The Microscopy ListServer -- CoSponsor: The
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} ----
} }
} } Germicidal lamps (short wavelength UV light) can
} } help make
} } hydrophobic grids somewhat more hydrophilic.
} } Basically, the UV
} } ionizes the air, generating reactive molecules (like
} } ozone) that then
} } react with the grid surfaces (possibly cleaning them
} } of undesirable
} } organics such as oils). Before we started using the
} } (much better) AC
} } glow-discharge method to make grids hydrophilic, we
} } did use this
} } method. Place the coated grids on a filter paper
} } (coated side facing
} } the lamp) about 6-12 inches away from the lamp for 5
} } minutes. They
} } should be used in a couple days as the effect wears
} } off.
} }
} } As with any new procedure, you will need to make
} } adjustments to the
} } distance and times since germicidal lamps vary in
} } strength and they
} } do become less effective as they age. I would start
} } by placing the
} } lamp 6 inches away from the grids and then
} } withdrawing a grid every
} } minute for so (4,5,6,7,8,9 min) and examining a
} } stained preparation
} } in the TEM looking for a relatively uniformly spread
} } stain with a
} } minimal number of patches of stain. Protect your
} } eyes (goggles) and
} } hands (gloves) from the UV and be aware that shiny
} } (stainless steel)
} } sturfaces reflect UV towards you.
} }
} } JB
} }
} } } Dear MSA, I have heard about using UV light to make
} } carbon coated grids
} } } hydrophilic. Can anyone give me some specifics and
} } perhaps some references?
} } } On or off list, as you wish.
} } }
} } } Thank you very much, Jeannette
} } }
} } } --
} } } Jeannette Taylor, Technologist II
} } } IM&MF
} } } Cherry L. Emerson Hall, Room E106
} } } Emory University
} } } 1515 Dickey Drive
} } } Atlanta, Georgia 30322
} }
} } --
} }
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++
} }
} } John J. Bozzola, Ph.D., Director
} } Integrated Microscopy & Graphics Expertise (IMAGE)
} } Southern Illinois University
} } 750 Communications Drive - MC 4402
} } Carbondale, IL 62901
} } Telephone: 618-453-3730
} }
} }
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++
} }
} } ==============================Original
} } Headers==============================
} } 7, 19 -- From bozzola-at-siu.edu Tue Jun 26 15:26:53
} } 2007
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} } (abbmta2.siu.edu [131.230.254.206])
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} } 7, 19 -- Date: Tue, 26 Jun 2007 15:26:50 -0500
} } 7, 19 -- To: Microscopy-at-msa.microscopy.com
} } 7, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu}
} } 7, 19 -- Subject: Re: [Microscopy] hydrophilic
} } carbon film
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} Headers==============================
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7, 25 -- From DusevichV-at-umkc.edu Thu Jun 28 08:43:08 2007
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From: DusevichV-at-umkc.edu
Date: Thu, 28 Jun 2007 08:54:07 -0500
Subject: [Microscopy] RE: thin sections in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Without STEM detector it is very difficult (impossible?) to get decent
image of thin sections in SEM. Anyway, I do use sometimes sections of
calcified tissue in SEM. Hydroxyapatite is visible with BSE, and when
grids are mounted on carbon, EDS spatial resolution in SEM is close to
that of STEM. So, I can get advantage of superior resolution of STEM
while working with multiple specimens in SEM.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
} Sent: Thursday, June 28, 2007 2:58 AM
} To: Dusevich, Vladimir
} Subject: [Microscopy] thin sections in SEM
}
}
}
}
} --------------------------------------------------------------
} --------------
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} Hi all!
}
} I discovered "demo" examples in our SEM and one example shows
} a TEM section with cells. The picture is very good (of course
} the demo example are rarely made of bad samples), very
} similar to what one can see by TEM. I wonder how it is
} possible to obtain good images of TEM section in SEM.
} I guess that it is only possible using BSE mode.
} Does anyone have experience with this? I wondered what would
} be the optimal thickness, resin, preparation.
} Are there special contrasting needs?
}
} Best regards,
}
} Stephane
}
}
}
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7, 25 -- From DusevichV-at-umkc.edu Thu Jun 28 08:54:07 2007
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From: dac-at-research.umass.edu
Date: Thu, 28 Jun 2007 09:12:58 -0500
Subject: [Microscopy] hydrophilic carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It might be good if the power (real, or setting) and Make/type of plasma
cleaner are specified for these discussions.

For the record, I use a Harrick PDC-3XG, and use it on the LOW setting
(nominally 6.8W - see mfg link below) for ~15 sec and get nicely
wettable pure carbon films. I apply the power as soon as the pump sounds
"right" (about 30 sec, probably ~ 0.1 Torr, glow discharge only happens
in a small window of pressure in these conditions) and if there is a
magenta glow I shut off the pump and time 15 sec. This discharge occurs
in the residual AIR. My films are always in the 5-10nm range (by Film
Thickness monitor and/or color). I treat grids multiple times with no
apparent degradation. I don't know how long the effect lasts - I see
various things suggesting that it lasts minutes to weeks - but I retreat
if they have been sitting more than 30 min because it is really easy and
they always work.

(The current model for this unit seems to be PDC-32G)

I have no connection to this company.
The manufacturer website is:
http://www.harrickplasma.com/products_cleaners.php

This unit has a needle valve fitting on the door so that materials can
be admitted to alter the surface properties.

Hope this helps.

Dale Callaham
The University of Massachusetts -at- Amherst





Michael.Fay-at-nottingham.ac.uk wrote:
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}
} You can use a plasma cleaner to do this, although normally you have to
} be careful you don't clean for longer than a few seconds if you're using
} grids with carbon films. However, with some plasma cleaner models there
} are holders available to buy that will decrease the rate, making the
} process more controllable.
}
} Dr Mike Fay
} Nottingham Nanotechnology and Nanoscience Centre
}
}
} -----Original Message-----
} X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
} Sent: 28 June 2007 08:55
} To: michael.fay-at-nottingham.ac.uk
} Subject: [Microscopy] hydrophilic carbon film
}
}
}
}
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}
} Is it not the principle of plasma cleaners?
} Could it be that this works to clean samples too?
}
} Regards,
}
} Stephane
}
} --- bozzola-at-siu.edu wrote:
}
} }
} }
} }
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} } Germicidal lamps (short wavelength UV light) can
} } help make
} } hydrophobic grids somewhat more hydrophilic.
} } Basically, the UV
} } ionizes the air, generating reactive molecules (like
} } ozone) that then
} } react with the grid surfaces (possibly cleaning them
} } of undesirable
} } organics such as oils). Before we started using the
} } (much better) AC
} } glow-discharge method to make grids hydrophilic, we
} } did use this
} } method. Place the coated grids on a filter paper
} } (coated side facing
} } the lamp) about 6-12 inches away from the lamp for 5
} } minutes. They
} } should be used in a couple days as the effect wears
} } off.
} }
} } As with any new procedure, you will need to make
} } adjustments to the
} } distance and times since germicidal lamps vary in
} } strength and they
} } do become less effective as they age. I would start
} } by placing the
} } lamp 6 inches away from the grids and then
} } withdrawing a grid every
} } minute for so (4,5,6,7,8,9 min) and examining a
} } stained preparation
} } in the TEM looking for a relatively uniformly spread
} } stain with a
} } minimal number of patches of stain. Protect your
} } eyes (goggles) and
} } hands (gloves) from the UV and be aware that shiny
} } (stainless steel)
} } sturfaces reflect UV towards you.
} }
} } JB
} }
} } } Dear MSA, I have heard about using UV light to make
} } carbon coated grids
} } } hydrophilic. Can anyone give me some specifics and
} } perhaps some references?
} } } On or off list, as you wish.
} } }
} } } Thank you very much, Jeannette
} } }
} } } --
} } } Jeannette Taylor, Technologist II
} } } IM&MF
} } } Cherry L. Emerson Hall, Room E106
} } } Emory University
} } } 1515 Dickey Drive
} } } Atlanta, Georgia 30322
} } --
} }
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++
} } John J. Bozzola, Ph.D., Director
} } Integrated Microscopy & Graphics Expertise (IMAGE)
} } Southern Illinois University
} } 750 Communications Drive - MC 4402
} } Carbondale, IL 62901
} } Telephone: 618-453-3730
} }
} }
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++
} }
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} } 2007
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} 8, 20 -- Subject: Re: [Microscopy] Re: hydrophilic carbon film
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} 19, 34 -- From Michael.Fay-at-nottingham.ac.uk Thu Jun 28 03:12:26 2007
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12, 23 -- From dac-at-research.umass.edu Thu Jun 28 09:12:58 2007
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From: rosslm-at-missouri.edu
Date: Thu, 28 Jun 2007 09:18:26 -0500
Subject: [Microscopy] Re: thin sections in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Have you tried lowering the KV? We recently got a FE SEM with STEM
capabilities and have experimented around with nanomaterials deposited on
C-caoted Cu grids under all KeV's and with both STEM and SE detection. You
never know what you will find but I've been amazed at some of the SE images.

Also, to boost the SE signal and if you have other samples, you could coat
the section with a metal to improve the SE signal, especially at lower
KeV's.

Thoughts from the dark side...
Lou Ross

On 6/28/07 2:59 AM, "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} wrote:

}
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} Hi all!
}
} I discovered "demo" examples in our SEM and one
} example shows a TEM section with cells. The picture is
} very good (of course the demo example are rarely made
} of bad samples), very similar to what one can see by
} TEM. I wonder how it is possible to obtain good images
} of TEM section in SEM.
} I guess that it is only possible using BSE mode.
} Does anyone have experience with this? I wondered what
} would be the optimal thickness, resin, preparation.
} Are there special contrasting needs?
}
} Best regards,
}
} Stephane
}
}
}
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} ==============================Original Headers==============================
} 6, 19 -- From nizets2-at-yahoo.com Thu Jun 28 02:58:01 2007
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} 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com}
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}

Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=2227
email: rosslm-at-missouri.edu
http://www.emc.missouri.edu


==============================Original Headers==============================
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7, 22 -- Date: Thu, 28 Jun 2007 09:18:23 -0500
7, 22 -- Subject: Re: [Microscopy] thin sections in SEM
7, 22 -- From: Lou Ross {rosslm-at-missouri.edu}
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From: reinhard.rachel-at-biologie.uni-regensburg.de
Date: Thu, 28 Jun 2007 09:35:50 -0500
Subject: [Microscopy] hydrophilic carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} It might be good if the power (real, or setting) and Make/type of
} plasma cleaner are specified for these discussions.

A comment from my side to this topic:

Since more than 15 years, I use a Harrick PDC-3XG plasma cleaner, in a
similar way as stated by Dale Callaham, of The University of
Massachusetts -at- Amherst, in his recent mail on the same topic.

I use it in the HIGH setting, for 15 sec, and get nicely wettable pure
carbon films and copper or gold grids, whatever is needed. Excellent
reproducability over the last years. Quick and efficient. Just perfect,
to my experience.
I have the possibility to keep the vacuum at about 0.1 to 1 mbar
(reading at a vacuum meter).

I think that the effect lasts for about 1 hour or two, but I did not
measure this. I just tell people / students to do it just before
applying the samples - say within 10 or 20 min. THis works fine.

I have no connection to this company.

best regards,

Reinhard Rachel
----------------------
PD Dr. Reinhard Rachel
Universitaet Regensburg
Centre for EM - NWF III -
Lehrstuhl fuer Anatomie
Universitaetsstr. 31
D-93053 Regensburg - Germany
tel +49 941 943 2837, 1720, 1666(TEM)
fax +49 941 943 2868
mail reinhard.rachel-at-biologie.uni-r.de
office: VKL 3.1.29



==============================Original Headers==============================
10, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Thu Jun 28 09:35:49 2007
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From: kraftpiano-at-gmail.com
Date: Thu, 28 Jun 2007 09:50:21 -0500
Subject: [Microscopy] SEM: Wet SEM capsule

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was recently perusing one of the supply catalogs for SEM supplies,
and I found a wet-cell capsule that has an "Electron transparent and
vacuum tight window."
(http://www.emsdiasum.com/microscopy/products/sem/wet/technical.aspx)
I was just wondering if anybody has used these, and what kind of
results they are getting.

The second part of my question is a bit more abstract. What quality
makes something electron transparent, and what materials are electron
transparent?

--Justin A. Kraft

==============================Original Headers==============================
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3, 26 -- Date: Thu, 28 Jun 2007 10:50:20 -0400
3, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com}
3, 26 -- To: microscopy-at-microscopy.com
3, 26 -- Subject: SEM: Wet SEM capsule
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From: NWWhite-at-bwxt.com
Date: Thu, 28 Jun 2007 10:11:42 -0500
Subject: [Microscopy] SEM: Wet SEM capsule

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Justin,

I have not used the capsule, so cannot comment on it.
Generally, a material becomes more transparent to electrons as the
atomic number / density drops and the material becomes thinner.

The capsule window cannot be completely electron transparent. From what
I read (if memory serves) it is not transparent to the low energy
electrons used for secondary imaging, but is transparent enough to let a
higher energy incident beam and the resultant BSEs to pass.

Same principle as the thin windows on EDS detectors that allow very low
energy x-rays to penetrate.

Woody

NW (Woody) White Jr
BWXT Services
Lynchburg, VA
http://www.bwxt.com/operations/semlab.html

-----Original Message-----
X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
Sent: Thursday, June 28, 2007 10:51 AM
To: White, Woody N.

I was recently perusing one of the supply catalogs for SEM supplies,
and I found a wet-cell capsule that has an "Electron transparent and
vacuum tight window."
(http://www.emsdiasum.com/microscopy/products/sem/wet/technical.aspx)
I was just wondering if anybody has used these, and what kind of
results they are getting.

The second part of my question is a bit more abstract. What quality
makes something electron transparent, and what materials are electron
transparent?

--Justin A. Kraft

==============================Original
Headers==============================
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From: LyudmilaSolovyeva-at-eaton.com
Date: Thu, 28 Jun 2007 19:32:46 -0500
Subject: [Microscopy] viaWWW: Commercial vendor for SEM/EDX service

Contents Retrieved from Microscopy Listserver Archives
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This Question/Comment was submitted to the Microscopy Listserver
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Email: LyudmilaSolovyeva-at-eaton.com
Name: Lyuda Solovyeva

Organization: EATON

Title-Subject: [Filtered] Commercial vendor for SEM/EDX service

Question: We use in our company LEO 1455 Variable Pressure SEM with Ixford INCA EDX system. We are looking for alternative Service contract for SEM/EDX. Could you, please, provide a list of vendors whi is available to do this job.

Thank you.

Lyuda Solovyeva
Lead Engineer - Eaton Innovation Center
Southfield, MI
Ph. 248-226-7179
Fax. 248-226-7165



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From: metzger-at-geosun.sjsu.edu
Date: Thu, 28 Jun 2007 19:33:13 -0500
Subject: [Microscopy] viaWWW: Electron microprobe - carbon coater

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Email: metzger-at-geosun.sjsu.edu
Name: Ellen Metzger

Organization: San Jose State University

Title-Subject: [Filtered] Electron microprobe - carbon coater

Question: We are in the process of setting up a probe lab and are in search of a carbon coater. I'd appreciate advice about carbon fiber versus rod and turbo pumped versus non-turbo for microprobe analysis of polished thin sections.



---------------------------------------------------------------------------

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From: frah0010-at-umn.edu
Date: Thu, 28 Jun 2007 20:17:57 -0500
Subject: [Microscopy] Re: viaWWW: Electron microprobe - carbon coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Ellen,

Our Electron Microprobe Lab has a JEOL JEE-400 Vacuum Evaporator,
mid-1990s vintage. It uses sharpened carbon rods and has a compound
rotary-diffusion vacuum system. The vacuum system valves are all
manual (no electronic automation), and the threat of cleaning burnt
diffusion-pump oil instills a healthy sense of fear in students.

A turbo pump will increase the cost of your coater by about 50% (last
time that I priced them), and it will be faster but not necessarily
any better. Diffusion pumps are hardy, and after 12 years or so, I
think we're on our third rotary pump (although we probably could have
repaired the broken ones if we were so inclined).

In our experience with samples coated in other facilities, the fiber-
type carbon coaters generally do not seem to apply as smooth a coat
as our rod-type coater. I usually insist that samples going in our
microprobe be coating in our lab, not elsewhere. This, though, is
based only on anecdotal evidence and our experiences, not any
systemic study.

Feel free to email me directly with any other questions, and you can
sign up for my microprobe-based listserver...

http://probelab.geo.umn.edu/listserver.html

... where you can seek advice from our microprobe users and facility
managers.

Best,
Ellery

--------------------
Ellery E. Frahm
Research Fellow & Manager
Electron Microprobe Laboratory
University of Minnesota - Twin Cities
Department of Geology & Geophysics
Lab Website: http://probelab.geo.umn.edu
Personal Website: http://umn.edu/~frah0010


On Jun 28, 2007, at 7:40 PM, metzger-at-geosun.sjsu.edu wrote:
}
} Email: metzger-at-geosun.sjsu.edu
} Name: Ellen Metzger
}
} Organization: San Jose State University
}
} Title-Subject: [Filtered] Electron microprobe - carbon coater
}
} Question: We are in the process of setting up a probe lab and are
} in search of a carbon coater. I'd appreciate advice about carbon
} fiber versus rod and turbo pumped versus non-turbo for microprobe
} analysis of polished thin sections.


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From: nizets2-at-yahoo.com
Date: Fri, 29 Jun 2007 04:26:23 -0500
Subject: [Microscopy] kV or keV?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,

In TEM the HT value is usually expressed in kV whereas
for SEM it is usually expressed in keV.
Being biologist, I don't easily comprehend the
difference between the 2 units.
In TEM the HT value represents the real tension
between the anode and the cathode. I expect that is
the same in SEM, so why add an "e" in-between? Does it
change anything, except the unecological use of extra
amount of ink?

Best regards,

Stephane



____________________________________________________________________________________
Got a little couch potato?
Check out fun summer activities for kids.
http://search.yahoo.com/search?fr=oni_on_mail&p=summer+activities+for+kids&cs=bz

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6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com}
6, 19 -- Subject: kV or keV?
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From: michael-at-shaffer.net
Date: Fri, 29 Jun 2007 04:44:43 -0500
Subject: [Microscopy] RE: kV or keV?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stephane asks ...

} In TEM the HT value represents the real tension between the
} anode and the cathode. I expect that is the same in SEM, so
} why add an "e" in-between? Does it change anything, ...

Tecnically, "kV" is a unit of potential energy, while "keV" is a unit of
kinetic energy because 'e' is supposed to include the mass of an electron.
In reality, we use them both interchangably.

HTH & Genuinely, Michael Shaffer :o)

SEM/MLA Research Coordinator
http://www.mun.ca/creait/maf/

Inco Innovation Centre
Memorial University
St. John's, Newfoundland



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8, 21 -- References: {200706290926.l5T9Qpqd025115-at-ns.microscopy.com}
8, 21 -- Subject: RE: [Microscopy] kV or keV?
8, 21 -- Date: Fri, 29 Jun 2007 07:14:27 -0230
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From: Philip.Koeck-at-biosci.ki.se
Date: Fri, 29 Jun 2007 05:55:11 -0500
Subject: [Microscopy] kV or keV?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

HT is a voltage and therefore always has the unit V(olt) or kV for kilo
Volt.
I recommend any SEMer who thinks otherwise to take a basic physics
course.

keV stands for kilo electronvolt, which is a (non-SI) energy-unit.
That's something completely different. To get from voltage to energy
you have to multiply with the charge of the particle traveling through
the potential-difference (or voltage).
1 eV is the kinetic energy of an electron that has been accelerated by
a voltage of 1 V. Just to make things clear: This is independent of the
electron's mass. It only depends on charge and voltage!

Hope that helps,

Philip


-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: 29 June 2007 11:30
To: Philip.Koeck-at-biosci.ki.se

Dear listers,

In TEM the HT value is usually expressed in kV whereas
for SEM it is usually expressed in keV.
Being biologist, I don't easily comprehend the
difference between the 2 units.
In TEM the HT value represents the real tension
between the anode and the cathode. I expect that is
the same in SEM, so why add an "e" in-between? Does it
change anything, except the unecological use of extra
amount of ink?

Best regards,

Stephane



==============================Original Headers==============================
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From: frah0010-at-umn.edu
Date: Fri, 29 Jun 2007 07:21:58 -0500
Subject: [Microscopy] kV or keV?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Technically, "kV" is a unit of potential energy, while "keV" is a
} unit of
} kinetic energy because 'e' is supposed to include the mass of an
} electron.
} In reality, we use them both interchangeably.

Except that students in my microprobe course who try to use these
terms interchangeably loose points. The same happens in the TEM
course taught at our university. It is the same difference between
potential and potential energy -- subtle but non-trivial.

Best,
Ellery

--------------------
Ellery E. Frahm
Research Fellow & Manager
Electron Microprobe Laboratory
University of Minnesota - Twin Cities
Department of Geology & Geophysics
Lab Website: http://probelab.geo.umn.edu
Personal Website: http://umn.edu/~frah0010


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5, 19 -- Subject: Re: [Microscopy] RE: kV or keV?
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From: dsoren-at-umich.edu
Date: Fri, 29 Jun 2007 08:05:11 -0500
Subject: [Microscopy] MSA schedule

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For the upcoming MSA meeting, is there a schedule yet as to specific
symposia and tutorial sessions? I am trying to decide on which days
to attend.

Dotty Sorenson

Dorothy Sorenson
Microscopy and Image-analysis Laboratory
Department of Cell and Developmental Biology
University Of Michigan Medical School
A807 BSRB
109 Zina Pitcher Place
Ann Arbor, MI 48109-2200
(734)763-1170
FAX (734)763-1166



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From: Andrew.Bowling-at-ARS.USDA.GOV
Date: Fri, 29 Jun 2007 08:23:48 -0500
Subject: [Microscopy] AFM opinions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello listers,

I was wondering if anyone on the listserve has any experience with the
Agilent 5500 series AFMs? I am especially interested to know how it
compares with the Veeco Multimode and/or Dimension...

Thank you,

Andy Bowling


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From: TindallR-at-missouri.edu
Date: Fri, 29 Jun 2007 09:06:25 -0500
Subject: [Microscopy] LN2 Dewars failing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

We recently had a 10L LN2 dewar lose vacuum and have had no luck
whatsoever finding someone who could pump it down at a reasonable (i.e.,
less than replacement) price. The dewar manufacturer said that they do
not (will not?) supply connectors for vacuum hoses so that we could
connect it to one of our pumps. Our own physics shop says that they
haven't pumped one for 15 years and it would cost hundreds of dollars
and lots of LN2 to do this.

Bottom line: buy a new dewar for $500+.

Can someone explain why this is such a huge problem? There is a vacuum
port on the dewar, and I assume it is there for a reason. I have seen
vacuum restored to EDS LN2 reservoirs by simply hooking them up to a
pump for basically the cost of the electricity to run the pump. Where
can we find connectors? Someone obviously makes them, but it seems to
be a well-kept secret.

Please reply to the list, since I expect I'm not the only one wandering
around in a vacuum and losing my cool.

Thanks much and Happy Fourth of July!

Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


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From: edelmare-at-muohio.edu
Date: Fri, 29 Jun 2007 09:19:08 -0500
Subject: [Microscopy] Re: MSA schedule

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The EXPO issue of Microscopy & Microanalysis is available on-line
with the detailed schedules for M&M 2007.

http://journals.cambridge.org/action/displayIssue?jid=MAM&volumeId=13&
issueId=S1

Hope that helps!

On 29 Jun 2007 at 8:06, dsoren-at-umich.edu wrote:

}
} For the upcoming MSA meeting, is there a schedule yet as to specific
} symposia and tutorial sessions? I am trying to decide on which days
} to attend.
}
} Dotty Sorenson
}
} Dorothy Sorenson
} Microscopy and Image-analysis Laboratory
} Department of Cell and Developmental Biology
} University Of Michigan Medical School
} A807 BSRB
} 109 Zina Pitcher Place
} Ann Arbor, MI 48109-2200
} (734)763-1170
} FAX (734)763-1166
}
}
}
} ==============================Original Headers==============================
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} 5, 16 -- Subject: MSA schedule
} 5, 16 -- Date: Fri, 29 Jun 2007 09:00:41 -0400
} 5, 16 -- X-Mailer: Apple Mail (2.752.2)
} ==============================End of - Headers==============================


Richard E. Edelmann, Ph.D.
EXPO Editor, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu


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From: edelmare-at-muohio.edu
Date: Fri, 29 Jun 2007 09:33:34 -0500
Subject: [Microscopy] Re: viaWWW: Electron microprobe - carbon coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ellen:

Turbo pumped systems pump faster. They pump to a lower pressure than
rotary pump only systems (i.e. table top models). Lower pressure
equals finer coating grain size.

Fiber (rope, cord, etc.) is a quick and easy carbon coat, and can
get away with higher pressures, but it is rougher, and can leave
"chunks" of fiber on sample. Not particularly controlable as to
thickness (use more fibers vs fewer fibers).

Rods systems tend to be finer coating, more controlable as to
"thickness" (evaporate more or less of the rod).

If you are thinking about EBSD work in the future, and might wish to
use a very thin carbon coat (yes, before someone yells out, it will
obsure some of the signal). The control of the rod systems is
needed.

I have not compared fiber vs rod for electron microprobe work on
polished sections. We use a full blown denton vacuum evaporator
(very fine, very versitile, very slow), and I really wish I could
get a turbo pumped table top model to easy speed for most SEM work. .
.


On 28 Jun 2007 at 19:33, metzger-at-geosun.sjsu.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
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}
} Email: metzger-at-geosun.sjsu.edu
} Name: Ellen Metzger
}
} Organization: San Jose State University
}
} Title-Subject: [Filtered] Electron microprobe - carbon coater
}
} Question: We are in the process of setting up a probe lab and are in
} search of a carbon coater. I'd appreciate advice about carbon fiber
} versus rod and turbo pumped versus non-turbo for microprobe analysis
} of polished thin sections.
}
}
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
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} 8, 11 -- From: metzger-at-geosun.sjsu.edu (by way of MicroscopyListserver)
} 8, 11 -- Subject: viaWWW: Electron microprobe - carbon coater
} 8, 11 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of - Headers==============================


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


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From: fortner-at-cmt.anl.gov
Date: Fri, 29 Jun 2007 09:47:50 -0500
Subject: [Microscopy] kV or keV?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

"kV" is kilovolts, an electrical potential (SI units). It is the
electric potential between the anode and cathode.

"keV" is kilo-electron-volts, a unit of energy (doesn't matter if
kinetic or potential). It represents the energy of a particle having a
unit (electron) charge in a 1 kV field (or its kinetic energy after
passing through a 1 kV field). Although not strictly SI, it is
considered a "unit accepted for use with SI"* It has nothing to do with
the particle's mass.

One can use either to describe the operating conditions of your SEM or
TEM interchangeably, since we're always accelerating electrons.

For EDS or EELS spectra, the unit is always keV or eV (unless an SI
purist prefers zeptojoules) since in the case of EDS one is referring to
the energy of an x-ray (chargeless and massless!), and in EELS one is
measuring **energy transfer** by an electron.

* See R. A. Nelson, "Guide for Metric Practice," Physics Today BG13
(1997).


"Before I came here I was confused about this subject. Having listened
to your lecture I am still confused, but on a higher level." - Enrico
Fermi

Jeffrey A. Fortner, Ph.D.
Chemical Engineering Division
Argonne National Laboratory
Argonne, IL 60439
fortner(at)cmt.anl.gov




-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Friday, June 29, 2007 4:27 AM
To: Fortner, Jeffrey A.

Dear listers,

In TEM the HT value is usually expressed in kV whereas for SEM it is
usually expressed in keV.
Being biologist, I don't easily comprehend the difference between the 2
units.
In TEM the HT value represents the real tension between the anode and
the cathode. I expect that is the same in SEM, so why add an "e"
in-between? Does it change anything, except the unecological use of
extra amount of ink?

Best regards,

Stephane



________________________________________________________________________
____________
Got a little couch potato?
Check out fun summer activities for kids.
http://search.yahoo.com/search?fr=oni_on_mail&p=summer+activities+for+ki
ds&cs=bz

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From: fortner-at-cmt.anl.gov
Date: Fri, 29 Jun 2007 09:47:51 -0500
Subject: [Microscopy] kV or keV?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

"kV" is kilovolts, an electrical potential (SI units). It is the
electric potential between the anode and cathode.

"keV" is kilo-electron-volts, a unit of energy (doesn't matter if
kinetic or potential). It represents the energy of a particle having a
unit (electron) charge in a 1 kV field (or its kinetic energy after
passing through a 1 kV field). Although not strictly SI, it is
considered a "unit accepted for use with SI"* It has nothing to do with
the particle's mass.

One can use either to describe the operating conditions of your SEM or
TEM interchangeably, since we're always accelerating electrons.

For EDS or EELS spectra, the unit is always keV or eV (unless an SI
purist prefers zeptojoules) since in the case of EDS one is referring to
the energy of an x-ray (chargeless and massless!), and in EELS one is
measuring **energy transfer** by an electron.

* See R. A. Nelson, "Guide for Metric Practice," Physics Today BG13
(1997).


"Before I came here I was confused about this subject. Having listened
to your lecture I am still confused, but on a higher level." - Enrico
Fermi

Jeffrey A. Fortner, Ph.D.
Chemical Engineering Division
Argonne National Laboratory
Argonne, IL 60439
fortner(at)cmt.anl.gov




-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Friday, June 29, 2007 4:27 AM
To: Fortner, Jeffrey A.

Dear listers,

In TEM the HT value is usually expressed in kV whereas for SEM it is
usually expressed in keV.
Being biologist, I don't easily comprehend the difference between the 2
units.
In TEM the HT value represents the real tension between the anode and
the cathode. I expect that is the same in SEM, so why add an "e"
in-between? Does it change anything, except the unecological use of
extra amount of ink?

Best regards,

Stephane



________________________________________________________________________
____________
Got a little couch potato?
Check out fun summer activities for kids.
http://search.yahoo.com/search?fr=oni_on_mail&p=summer+activities+for+ki
ds&cs=bz

==============================Original
Headers==============================
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From: jquinn-at-www.matscieng.sunysb.edu
Date: Fri, 29 Jun 2007 11:36:01 -0500
Subject: [Microscopy] re: carbon coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ellen and Folks

When one of your students blows the turbo pump,
you'll wish you had a diffusion pump. The diff-pump
will last longer at a significant cost reduction.
However, most are water cooled, and some facilities
people don't like to see you dumping water continuously
down the drain.

JQuinn


} From mail-at-ns.microscopy.com Thu Jun 28 20:29:42 2007
} Date: Thu, 28 Jun 2007 19:33:54 -0500
} To: jquinn-at-www.matscieng.sunysb.edu
} From: metzger-at-geosun.sjsu.edu
} Reply-to: metzger-at-geosun.sjsu.edu
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} Subject: [Microscopy] viaWWW: Electron microprobe - carbon coater
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} Email: metzger-at-geosun.sjsu.edu
} Name: Ellen Metzger
}
} Organization: San Jose State University
}
} Title-Subject: [Filtered] Electron microprobe - carbon coater
}
} Question: We are in the process of setting up a probe lab and
are in search of a carbon coater. I'd appreciate advice about
carbon fiber versus rod and turbo pumped versus non-turbo for
microprobe analysis of polished thin sections.
}
}
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 11 -- From zaluzec-at-microscopy.com Thu Jun 28 19:33:12 2007
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} 8, 11 -- Subject: viaWWW: Electron microprobe - carbon coater
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==============================Original Headers==============================
8, 12 -- From jquinn-at-www.matscieng.sunysb.edu Fri Jun 29 11:36:01 2007
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8, 12 -- Subject: re: carbon coater
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From: dac-at-research.umass.edu
Date: Fri, 29 Jun 2007 11:42:58 -0500
Subject: [Microscopy] viaWWW: Electron microprobe - carbon coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've got to take issue with this disparaging information about carbon
string evaporation.

Carbon string gets criticized in the way given below, and I think that
some of this comes from using it with rotary pumped vacuum levels, but
possibly also the method of evaporation; the method usually given is
"flash evaporation". We pump down to at least 2x10^-5 Torr (~ 0.0027 Pa)
and evaporate in a series of pulses that bring the string to just
white-hot and then switch off for a couple of seconds, repeating until
the string "sparkles" the final remaining fibers. I have found the
method highly repeatable and easy to arrive at a repeatable thickness.
Call this "pulsed evaporation"?

We use 2 different systems , neither designed for carbon string. One is
a Balzers BA080T turbo-pumped unit, and the other is a Kinney KDP-2A
(circa 1965) ODP pumped system. For the Kinney, easier to explain, I use
a short 1.25cm spacing tungsten boat holder (like the Fullam No. 12330:
EFFA® Tungsten Boat Kit) and just lightly clamp the carbon string (SPI
#11433 - 0.8mm dia) where the boat would normally go - 1.25 cm exposed
length. This is set at a distance chosen for the desired thickness, ~5.5
-8cm typical. The system is pumped down to below 1x10^-4 Torr (2x10^-5
Torr typ.) and the string is degassed by slowly raising the voltage to
give a medium bright orange glow - much faster vacuum recovery than with
rods - and then switched OFF. The Variac (controls the input to the
power transformer) is then set for ~40% (without power applied!) (this
is 40% of max, max being 24VAC nominal of the power transformer
secondary). The switch is flipped ON (view through dark glass, and as
soon as it seems to have risen to white and stable temperature (~0.5s),
switch off, repeating this cycle after a few seconds until burnout. This
method was chosen to allow the carbon to reach evaporation temperature,
but not send it all off at once. The radiation heating is minimized and
spread over time. I coat the "back side" of Formvar that is placed over
5mm holes (for the method of picking up sections with a 1x2mm slot grid
and setting the grid on the upper non-carbon-coated side of the filmed
area) and I see almost no damaged films using this method, and had much
trouble trying this with rods (1/8", turned down to 1mm dia x 2mm long
tips).

We use a bit of very white paper stock stuck under the mica, etc., as an
indicator of thickness by color; looking through my notebook, it is
highly repeatable. In making adjustments to vary thickness, keep in mind
that the thickness is inverslely proportional to the SQUARE of the
distance; a small change in distance has a substantial effect on the
film thickness.

By setting the voltage and pulsing time appropriately, it should be
possible to get good coverage of rotating samples as well.

Different yarns can also be used, selecting a voltage to give similar
characteristics to the evaporation. It seems that the SPI #11433 thread
just comes to a stable white heat in ~0.5s, and that will vary for
different product diameters. Most EM supply vendors carry a selection
of thicknesses. The "thread" mentioned above gives a nice balance
between thickness and heating. Thicker thread could be used at longer
distances. One could experiment with different lengths as well; we have
good success with the 1.25cm length. The paper cited below was very
specific about the lengths used - he did NOT get good results with the
length we use, but he did use "flash" evaporation. Unfortunately,
technical data regarding the voltages and type of power supply are not
given in that paper.

===================================================
Precise and reproducible deposition of thin and ultra-thin carbon films
by flash evaporation of carbon yarn in high vacuum. Klaus-Ruediger
Peters. J. Micros. (133), p 17. 1984.

His "gun" design was a covered chamber that included a yarn reservoir.
The yarn was clamped between 2 posts 6mm separated, and the yarn was
exposed through a hole. Details in paper... With the cover removed it
gave ~50% thicker films (does not state if quality differences)
Shorter lengths gave thicker films...
{5 mm length emitted as a shower of sparks, gave substantial variations
in thickness
} 9 mm lengths also resulted in high variation in measured thickness.
6 mm gave the most reliable depositions.
======================================================

There are some things I don't fully understand in this paper - like that
shorter lengths gave thicker films??? - maybe for shorer than 5mm
lengths MOST fibers fully span the electrodes and it changes the
characteristics of evaporation.

But if you have the apparatus to try this method, I strongly encourage
trying it. It is economical and repeatable and the film quality is
excellent.

Dale Callaham
The University of Massachusetts -at- Amherst



edelmare-at-muohio.edu wrote:
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} Ellen:
}
} Turbo pumped systems pump faster. They pump to a lower pressure than
} rotary pump only systems (i.e. table top models). Lower pressure
} equals finer coating grain size.
}
} Fiber (rope, cord, etc.) is a quick and easy carbon coat, and can
} get away with higher pressures, but it is rougher, and can leave
} "chunks" of fiber on sample. Not particularly controlable as to
} thickness (use more fibers vs fewer fibers).
}
} Rods systems tend to be finer coating, more controlable as to
} "thickness" (evaporate more or less of the rod).
}
} If you are thinking about EBSD work in the future, and might wish to
} use a very thin carbon coat (yes, before someone yells out, it will
} obsure some of the signal). The control of the rod systems is
} needed.
}
} I have not compared fiber vs rod for electron microprobe work on
} polished sections. We use a full blown denton vacuum evaporator
} (very fine, very versitile, very slow), and I really wish I could
} get a turbo pumped table top model to easy speed for most SEM work. .
} .
}
}
} On 28 Jun 2007 at 19:33, metzger-at-geosun.sjsu.edu wrote:
}
} }
} }
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} }
} } Organization: San Jose State University
} }
} } Title-Subject: [Filtered] Electron microprobe - carbon coater
} }
} } Question: We are in the process of setting up a probe lab and are in
} } search of a carbon coater. I'd appreciate advice about carbon fiber
} } versus rod and turbo pumped versus non-turbo for microprobe analysis
} } of polished thin sections.
} }
} }
} }
} } ---------------------------------------------------------------------------
} }
} } ==============================Original Headers==============================
} } 8, 11 -- From zaluzec-at-microscopy.com Thu Jun 28 19:33:12 2007
} } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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} } 8, 11 -- Subject: viaWWW: Electron microprobe - carbon coater
} } 8, 11 -- Content-Type: text/plain; charset="us-ascii"
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}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Director
} 364 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}
} ==============================Original Headers==============================
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==============================Original Headers==============================
14, 22 -- From dac-at-research.umass.edu Fri Jun 29 11:42:58 2007
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14, 22 -- Date: Fri, 29 Jun 2007 12:45:49 -0500
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From: drteddunne-at-yahoo.com
Date: Fri, 29 Jun 2007 12:15:46 -0500
Subject: [Microscopy] re: carbon coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You don't need to "dump water down the drain". Use a cooler/circulator to cool the diff pump.

Ted Dunn

----- Original Message ----
X-from: "jquinn-at-www.matscieng.sunysb.edu" www.matscieng.sunysb.edu}
To: drteddunne-at-yahoo.com
Sent: Friday, June 29, 2007 11:39:39 PM


Ellen and Folks

When one of your students blows the turbo pump,
you'll wish you had a diffusion pump. The diff-pump
will last longer at a significant cost reduction.
However, most are water cooled, and some facilities
people don't like to see you dumping water continuously
down the drain.

JQuinn


} From mail-at-ns.microscopy.com Thu Jun 28 20:29:42 2007
} Date: Thu, 28 Jun 2007 19:33:54 -0500
} To: jquinn-at-www.matscieng.sunysb.edu
} From: metzger-at-geosun.sjsu.edu
} Reply-to: metzger-at-geosun.sjsu.edu
} X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com}
} Subject: [Microscopy] viaWWW: Electron microprobe - carbon coater
} Errors-To: MicroscopyListSpamFilter-at-microscopy.com
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}
}
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} Email: metzger-at-geosun.sjsu.edu
} Name: Ellen Metzger
}
} Organization: San Jose State University
}
} Title-Subject: [Filtered] Electron microprobe - carbon coater
}
} Question: We are in the process of setting up a probe lab and
are in search of a carbon coater. I'd appreciate advice about
carbon fiber versus rod and turbo pumped versus non-turbo for
microprobe analysis of polished thin sections.
}
}
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 11 -- From zaluzec-at-microscopy.com Thu Jun 28 19:33:12 2007
} 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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} 8, 11 -- Subject: viaWWW: Electron microprobe - carbon coater
} 8, 11 -- Content-Type: text/plain; charset="us-ascii"
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==============================Original Headers==============================
8, 12 -- From jquinn-at-www.matscieng.sunysb.edu Fri Jun 29 11:36:01 2007
8, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33])
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8, 12 -- Date: Fri, 29 Jun 2007 12:31:40 -0400
8, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu}
8, 12 -- Message-Id: {200706291631.l5TGVen28841-at-www.matscieng.sunysb.edu}
8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- Subject: re: carbon coater
==============================End of - Headers==============================






____________________________________________________________________________________
Sick sense of humor? Visit Yahoo! TV's
Comedy with an Edge to see what's on, when.
http://tv.yahoo.com/collections/222

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20, 20 -- Subject: Re: [Microscopy] re: carbon coater
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From: ionsourcerer-at-mac.com
Date: Fri, 29 Jun 2007 12:19:22 -0500
Subject: [Microscopy] Failing LN2 Dewars

Contents Retrieved from Microscopy Listserver Archives
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Fwiw: It really shouldn't be all that hard with a bit of the right
vacuum plumbing.
I've been kluging stuff together for years out of a combination of
economic
necessity, and ultimately sheer pig-headedness and principle.

It strictly depends on whether 'doing it properly' or 'getting it
done' is a higher
priority. Your shop folks doubtless think you expect the former,
since most
people do.

If you go back and let them know that you are only concerned with the
latter,
and not cosmetics or perfection, they should be able to help you out
nicely.
The difference in thermal loss between a 'hard vacuum' and a 'soft
vacuum'
may mean you have to top it off a bit more frequently, but not by much.
$500 will buy more than enough LN2 to cover the difference for years.

I don't want to tell your hardworking folks in the shop how to do
their job,
and every situation is unique anyway. If you can live with 'Plan B'
run it
past them again on a 'best efforts' basis.

If you strike out for one reason or another get back to me and I'll
be happy
to give you a hand.

Cheers,

b.

Rick Becker
Cluster Sciences, L.L.C.
Borolene Metamaterials
39 Topsfield Rd.
Ipswich, MA 01938 US
978-337-9009
ionsourcerer-at-mac.com

If you don't know where you are going, call it "exploration".
If you don't know what you are doing, call it "research".


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11, 23 -- Subject: Failing LN2 Dewars
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From: cannonmp-at-comcast.net
Date: Fri, 29 Jun 2007 13:10:49 -0500
Subject: [Microscopy] LN2 Dewar Valves

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I had been plagued with the dewar valve problem for several years before I
found my own solution. Not even Duniway Stockroom will sell you an adaptor.

Cut the valve stem off and heliarc a KF fitting onto your new open port.
The new fitting will allow you to outfit the opening with anything you can
imagine. Vacuum gauges and valves of any style. My old 16 liter dewar will
keep LN2 in it for three months if stored in a household refrigerator.

Early on I pumped the dewar to the low 10-3s. Now I just use a roughing
pump. No difference in hold time.

I've done something similar with my early 1980s KEVEX EDS dewar. I've
connected it to my system vacuum, but isolated with an ordinary vacumm
valve. When it's dedicated thermocouple gauge climbs above 10 microns, I
pump it down. Still has 159 ev at Mn. The original specification.

Bart Cannon
Cannon Microprobe
Seattle


==============================Original Headers==============================
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From: microscopytoday-at-tampabay.rr.com
Date: Fri, 29 Jun 2007 14:16:54 -0500
Subject: [Microscopy] Microscopy Today July Table of Contents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

Here is the July 2007 Microscopy Today table of contents. I will close
the subscription list for this issue on Wednesday, July 6th, 2007.

Microscopists in North America and MSA members anywhere can subscribe
for free. Anyone else may subscribe for US$50 per year (to PARTIALLY
cover postage). All subscriptions at http://www.microscopy-today.com
Thank you.

Ron Anderson, Editor
================================
Probing Individual Proteins in Unsupported Membranes
Stephen W. Carmichael, Mayo Clinic, Rochester, MN

Microbeam Analyses of the Most Challenging Extraterrestrial Samples Ever
Returned
Frans J.M. Rietmeijer, U. of New Mexico, Albuquerque, NM

Microscopy Today 2007 Salary Survey Results
Ron Anderson and Barbara Foster,* Microscopy Today and
*Microscopy/Microscopy &Education, Inc.

Fibrous Sepiolite Use As An Asbestos Substitute: Analytical Basics
Louis Solebello, Int'l Asbestos Testing Lab. Inc., Mt. Laurel, NJ

Low Vacuum SEMs: Latest Generation Technologies and Applications
William Neijssen, Ben Lich, & Pete Carleson* FEI Company, Eindhoven, The
Netherlands and *Hillsboro, OR, USA

Luminance Contrast – a New Visible Light Technique for Examining
Transparent Specimens
Jörg Piper, Clinic Meduna, Bad Bertich, Germany

Temporal Evolution of Incipient Damage on Metallic Surfaces Analyzed by
Unidirectional Laser Oblique Illumination (ULOI)
E.A. Favret1,2, N.O. Fuentes2,3, & L. Ferrero2,1; Inst. de Tecnología
Agropecuaria (INTA), 2Univ. Nacional de Gral San Martin, 3Comisión
Nacional de Energía Atómica, Buenos Aires. Argentina

Multimode Trans-Illuminator for the Stereomicroscope
Ted Clarke, Retired Materials Engineer

New Approaches to Managing, Marketing, and Money for Maintaining a Core
Facility
Developing a Financial Plan for the Long-term “Care and Feeding” of
Major Equipment
Alberto Rodriguez and Charlene Sullivan, Krannert School of Management,
Purdue University

The College of Microscopy – Meeting Rapidly Growing Microscopy Demands
Donald A. Brooks, The McCrone Group, Westmont, Illinois

Getting Epoxy Semi-Thin Sections to Stick to Glass Slides
Gilbert (Gib) Ahlstrand, Univ. of Minnesota, St. Paul, MN

SEM Stub Holders for Sputter Coating at 90° Tilt
Amanda Best, Central Michigan University, Mt. Pleasant, MI

Digital Cameras and the TEM
Warren Straszheim, Iowa State University, Ames, Iowa

Silicon Cross-Section Sample Preparation (Cleaving)
Richard Beanland, Materials Analysis, Bookham, U.K.

Industry News

NetNotes
SPECIMEN PREPARATION - cell cultures and aldehyde fixatives
SPECIMEN PREPARATION - lung tissue
SPECIMEN PREPARATION – enhancing contrast of mitochondria
SPECIMEN PREPARATION - plant fixation
SPECIMEN PREPARATION - cacodylate vs. phosphate buffer
SPECIMEN PREPARATION - myelin osmication
SPECIMEN PREPARATION - alternative to uranyl acetate stain
SPECIMEN PREPARATION - lead citrate and block staining
SPECIMEN PREPARATION - dehydration of molds
SPECIMEN PREPARATION – ruthenium oxide
SPECIMEN PREPARATION - bacterial flagella
SPECIMEN PREPARATION - bacterial samples for SEM
SPECIMEN PREPARATION - cationic dyes for bacterial visualization in SEM
SPECIMEN PREPARATION - progressive lowering of temperature technique
SPECIMEN PREPARATION - metal shadow casting of DNA
SPECIMEN PREPARATION – cross-sectioning packaging film
SPECIMEN PREPARATION - aluminum evaporation
SPECIMEN PREPARATION – sputter coating
SPECIMEN PREPARATION – humidity levels
IMAGING – digital compression
XTEM - surface densities of particles
SEM - smooth, low-Z substrates
SEM - imageing on glass
SEM - aluminosilicate and BSE
SEM - intermittent vacuum leak
STEM-EDX
EDS - carbon contamination
ELECTRON DIFFRACTION - instability
ELECTRON DIFFRACTION - orientation of the diffraction pattern

Advertiser's Index


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From: metzger-at-geosun.sjsu.edu
Date: Fri, 29 Jun 2007 18:26:46 -0500
Subject: [Microscopy] viaWWW: Thanks to all who responded to my previous posting - the

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
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Email: metzger-at-geosun.sjsu.edu
Name: Ellen Metzger

Organization: San Jose State University

Title-Subject: [Filtered] Electron microprobe - carbon coater

Question: Thanks to all who responded to my previous posting - the information is most helpful.

I have one additional query. My colleagues in Engineering have a carbon coater for their SEM samples. Is their a difference in the requirements and thus the type of coater needed for SEM vs. electron microprobe (polished thin sections) samples?


---------------------------------------------------------------------------

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From: jeff-at-metallography.com
Date: Fri, 29 Jun 2007 18:27:13 -0500
Subject: [Microscopy] viaWWW: metallography contest deadline July 25

Contents Retrieved from Microscopy Listserver Archives
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Email: jeff-at-metallography.com
Name: Jeff Stewart

Organization: International Metallographic Society

Title-Subject: [Filtered] metallography contest deadline July 25

Question: A reminder that The International Metallographic Contest, co-sponsored by IMS and ASM International, is being held in conjunction with the M&M 2007 meeting in Ft. Lauderdale. The Contest has nine categories of competition which are primarily structured for materials analysts, and three categories for artistic photomicrographs. Best in Show will receive the Jacquet-Lucas Award and $3000.00. First Place winners in each category will receive $200.00. The Second Place winners will receive $100.00. Third Place winners will receive $50.00. A certificate of appreciation will be awarded for Honorable Mention. First Place undergraduate student winners in Classes 7 and 8 also receive the George L. Kehl Plaque.

It's not too late to enter. If you've already authored a poster presentation for the meeting, consider downsizing it to 16x20 and entering it in the IMC. If you've got a great looking photomicrograph, enter it in one of the artistic categories. Complete rules are available at www.internationalmetallographicsociety.org/contest.html. Mail entries to Bonnie Koske, HEICO Aerospace, 3000 Taft Street, Hollywood, FL 33021. Deadline is July 25.

Contact me off list if you have any questions.

Good luck to all entrants.

Jeff Stewart
International Metallographic Contest Chair
Metallographic Laboratory Manager
Stern-Leach Company
49 Pearl Street
Attleboro, MA 02703
Phone: 508-222-7400 x1329


---------------------------------------------------------------------------

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From: ceren_b68-at-hotmail.com
Date: Fri, 29 Jun 2007 22:06:30 -0500
Subject: [Microscopy] LECTIN HELP

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

No, the same units would be used for both applications with the same
caveats as discussed. A probe sample _might_ require a bit more carbon
than an SEM sample due to the probe currents involved, but the same
coaters could be used.

Warren

-----Original Message-----
X-from: metzger-at-geosun.sjsu.edu [mailto:metzger-at-geosun.sjsu.edu]
Sent: Friday, June 29, 2007 6:28 PM
To: wesaia-at-iastate.edu

Hi, everyone
I am a M.S student and I would like to study the bacterial cytoskeletal
elements for various bacteria (E. coli for the moment). I
would like to label FtsZ, MreB or such proteins with lectins and then label
them with gold or directly label these proteins with
gold-labelled lectins and then observe the results in TEM.

Does anyone know any specific lectins for bacterial cytoskeletal proteins or
anything about this subject?

Any help is appreciated
Thank you in advance


Ceren Buyukkayalar
M.S student
Gebze Institute of Technology
Turkey

_________________________________________________________________
Don’t miss your chance to WIN $10,000 and other great prizes from Microsoft
Office Live http://clk.atdmt.com/MRT/go/aub0540003042mrt/direct/01/


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From: drteddunne-at-yahoo.com
Date: Sat, 30 Jun 2007 03:05:32 -0500
Subject: Re: [Microscopy] re: carbon coater

Contents Retrieved from Microscopy Listserver Archives
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I once had a carbon coater with diff pump that I needed to cool independently from a TEM. I bought a domestic drinking water cooler and used a small submersible pump in a tank to circulate the water. It was quite cheap to put together and worked well.

Ted Dunn


----- Original Message ----
X-from: Jim Quinn www.matscieng.sunysb.edu}
To: drteddunne-at-yahoo.com
Sent: Saturday, June 30, 2007 12:43:55 AM




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