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Email: modla-at-dbi.udel.edu Name: Shannon Modla
Organization: Delaware Biotechnology Institute
Title-Subject: [Filtered] Metal Shadow Casting of DNA
Question: We are currently trying to rotary shadow cast DNA but are having problems obtaining consistent results with the metal shadowing. We are using an 80:20 platinum palladium wire purchased from EMS and a Denton Bench Top Turbo III sputter coater. The platinum palladium wire was wrapped around a 2-3 cm portion of a hairpin loop formed with tungsten wire. When the filament power is increased, the platinum palladium wire will either heat up without melting and evaporating or else the tungsten wire breaks without appreciable metal shadowing. I was wondering if anyone had any experience with this technique and could offer suggestions about how to obtain more consistent results.
Thanks,
Shannon Modla Delware Biotechnology Institute BioImaging Center
The geology department is looking for recommendation on getting a Cathodoluminescence-Optical Microscope for geological research purpose. Samples to be view would include but not limited to Zircon, Quartz, and carbonates. We appreciate any recommendations and experiences you may have on such instrumentation. Thanks in advance!
Best Regards, ---------------------------------------------- Xiang Yang Electron Microscopy Center Saint Mary's University Science Building, Suite 135 Halifax, NS Canada B3H 3C3 Tel: (902) 496-8292 (lab) (902) 420-5709 (off) http://fgsr.smu.ca/emc/
==============================Original Headers============================== 4, 21 -- From xyang-at-SMU.CA Fri Jun 1 08:32:08 2007 4, 21 -- Received: from HUSKY8.SMU.CA (Husky8.smu.ca [140.184.1.8]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51DW7HP007335 4, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 08:32:08 -0500 4, 21 -- Received: from s101tech ("port 4931"-at-[140.184.73.254]) 4, 21 -- by HUSKY1.SMU.CA (PMDF V6.2-X25 #30841) 4, 21 -- with SMTP id {01MH9S50LTZC8Z2TIS-at-HUSKY1.SMU.CA} for Microscopy-at-microscopy.com; 4, 21 -- Fri, 01 Jun 2007 10:32:06 -0300 4, 21 -- Date: Fri, 01 Jun 2007 10:31:38 -0300 4, 21 -- From: Xiang Yang {xyang-at-SMU.CA} 4, 21 -- Subject: vendors on CL-microscope 4, 21 -- To: Microscopy-at-microscopy.com 4, 21 -- Reply-to: Xiang Yang {xyang-at-SMU.CA} 4, 21 -- Message-id: {007b01c7a451$405ab0a0$fe49b88c-at-stmarys.smunet.smu.ca} 4, 21 -- MIME-version: 1.0 4, 21 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 4, 21 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3028 4, 21 -- Content-type: text/plain; reply-type=original; charset=iso-8859-1; format=flowed 4, 21 -- Content-transfer-encoding: 7bit 4, 21 -- X-Priority: 3 4, 21 -- X-MSMail-priority: Normal ==============================End of - Headers==============================
Our UC-4 has never been re-evacuated. It has a yellow plastic cap over the port, the port is filled with a pink grease, and under the pink grease it looks like a metal ball. I can't say any more, but it looks like the metal ball is original.
Diane Ciaburri
-----Original Message----- X-from: oddioeng-at-aol.com [mailto:oddioeng-at-aol.com] Sent: Thursday, May 31, 2007 10:53 PM To: Ciaburri, Diane A.
What for did you "spin 200nm PMMA on the surface?" As I understand, you have just glassy flat surface of resin on your specimen, with no topography and nothing to observe.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} } Email: yitianp-at-ece.arizona.edu } Name: Yitian Peng } } Organization: University of Arizona } } Title-Subject: [Filtered] Question about image on Glass. } } Question: Hi everyone, } I have some patterned Cr structure(width:100micro, } height:60nm) on Glass. } Then I spin 200nm PMMA on the surface. } Then I Sputter 10nm Cr on the PMMA. } I want to using JSM 6400 SEM to look at the structure. } But I can't see anything?/ } What's the problem?/ } Thank you very much. } Yitian } } } -------------------------------------------------------------- } ------------- } } ==============================Original } Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Thu May 31 21:52:36 2007 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id l512qZ3N011694 } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 31 May } 2007 21:52:35 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p0624080ac2853a635b12-at-[206.69.208.22]} } 7, 11 -- Date: Thu, 31 May 2007 21:52:34 -0500 7, 11 -- To: } microscopy-at-microscopy.com 7, 11 -- From: } yitianp-at-ece.arizona.edu (by way of MicroscopyListserver) 7, } 11 -- Subject: viaWWW: Question about image on Glass 7, 11 -- } Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers============================== }
==============================Original Headers============================== 6, 25 -- From DusevichV-at-umkc.edu Fri Jun 1 08:54:07 2007 6, 25 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51Ds7jf030569 6, 25 -- for {microscopy-at-msa.microscopy.com} ; Fri, 1 Jun 2007 08:54:07 -0500 6, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.1830); 6, 25 -- Fri, 1 Jun 2007 08:54:07 -0500 6, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 25 -- Content-class: urn:content-classes:message 6, 25 -- MIME-Version: 1.0 6, 25 -- Content-Type: text/plain; 6, 25 -- charset="us-ascii" 6, 25 -- Subject: RE: [Microscopy] viaWWW: Question about image on Glass 6, 25 -- Date: Fri, 1 Jun 2007 08:54:06 -0500 6, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB33ADCAA-at-KC-MSX1.kc.umkc.edu} 6, 25 -- In-Reply-To: {200706010253.l512rpk7013748-at-ns.microscopy.com} 6, 25 -- X-MS-Has-Attach: 6, 25 -- X-MS-TNEF-Correlator: 6, 25 -- Thread-Topic: [Microscopy] viaWWW: Question about image on Glass 6, 25 -- Thread-Index: Acej+Bjmiqw1+i/PT7aFRDMN0xOWGQAW3m3w 6, 25 -- References: {200706010253.l512rpk7013748-at-ns.microscopy.com} 6, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 6, 25 -- To: {yitianp-at-ece.arizona.edu} , {microscopy-at-msa.microscopy.com} 6, 25 -- X-OriginalArrivalTime: 01 Jun 2007 13:54:07.0130 (UTC) FILETIME=[527A3FA0:01C7A454] 6, 25 -- Content-Transfer-Encoding: 8bit 6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l51Ds7jf030569 ==============================End of - Headers==============================
I'm not entirely sure if this is appropriate for this list, and if it isn't I apologize in advance. I was just informed yesterday that my position as science teacher at Atlantis Academy has been eliminated, and that the math teacher will be teaching both subjects. It is unfortunately a sad commentary on the direction that our world is headed in.
I am making an appeal to anyone in the south Florida area who may need some extra lab help, or anything that I can do. My prospects for getting a new teaching job are not good until later in the summer, but I would really like to start working somewhere locally as a lab assistant so I can start working on my masters degree.
I know this is a bit of a shot in the dark, but I figured there'd be no harm in asking.
Thank you all for your kind support over the last few months on our SEM project. I am truly sorry that I will not be able to see it come to fruition.
--Justin A. Kraft
==============================Original Headers============================== 5, 26 -- From kraftpiano-at-gmail.com Fri Jun 1 09:06:48 2007 5, 26 -- Received: from nz-out-0506.google.com (nz-out-0506.google.com [64.233.162.233]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51E6ltZ009776 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 09:06:48 -0500 5, 26 -- Received: by nz-out-0506.google.com with SMTP id m7so462802nzf 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 01 Jun 2007 07:06:47 -0700 (PDT) 5, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 5, 26 -- d=gmail.com; s=beta; 5, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 26 -- b=qs9vE5p9pcEXK/Msl9ElX2ktGlmc8jozq5wszsRSWN6ArbUwJLxxunGvssS2tFtDtss14oERxMeGxC8fTGBaTlND/3bD+Vg5XHffF3pR37edRsswSjQ7LI4Szh9Tvq3pT3wHKR8m1T6QOUn6G9faTwZdu/ZbAAmmuFP0/iV+P9E= 5, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 26 -- d=gmail.com; s=beta; 5, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 26 -- b=JqpAB/S5rMJwRtokakle1G6MLLcW4qbSMLkqmmmV3QgTez0J6QNOS6g2A2gW3T6rUw6y2F1MQiyWxmxjafDGdQzC7+GBxAA+KeINaSr9z8iDh+phCl1cO4lkBTjEKFPnrJ7VFK1DhociSHCDTrabJMf9nVLRJkflzXEhpqGouYQ= 5, 26 -- Received: by 10.114.148.1 with SMTP id v1mr1863566wad.1180706807173; 5, 26 -- Fri, 01 Jun 2007 07:06:47 -0700 (PDT) 5, 26 -- Received: by 10.114.78.15 with HTTP; Fri, 1 Jun 2007 07:06:46 -0700 (PDT) 5, 26 -- Message-ID: {25e2b0d20706010706r5763fc81gfb6396e2d59d09c7-at-mail.gmail.com} 5, 26 -- Date: Fri, 1 Jun 2007 10:06:46 -0400 5, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 5, 26 -- To: microscopy-at-microscopy.com 5, 26 -- Subject: Unfortunate announcement. 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
I was once given the task of looking at a glassy surface in the SEM. Just to rough in the focus was a challenge. I sprinkled some powder on the surface to provide a starting point for focus. Maybe this would help you get started.
Otherwise, I agree with Vladimir... that PMMA - though optically transparent - is not electron transparent, and all you'll see is the Cr-sputter-coated polymer surface.
Stu Smalinskas, P.E. Metallurgist SKF USA Plymouth, Michigan (734)414-6862
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Vladimir wrote:
What for did you "spin 200nm PMMA on the surface?" As I understand, you have just glassy flat surface of resin on your specimen, with no topography and nothing to observe.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
Title-Subject: [Filtered] Question about image on Glass.
Question: Hi everyone, I have some patterned Cr structure (width: 100micro, height: 60nm) on Glass. Then I spin 200nm PMMA on the surface. Then I Sputter 10nm Cr on the PMMA. I want to using JSM 6400 SEM to look at the structure. But I can't see anything? What's the problem?
Thank you very much.
Yitian
Email: yitianp-at-ece.arizona.edu Name: Yitian Peng Organization: University of Arizona
____________________________________________________________________________________ Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. http://answers.yahoo.com/dir/?link=list&sid=396546091
==============================Original Headers============================== 18, 19 -- From smalinskas-at-yahoo.com Fri Jun 1 09:44:16 2007 18, 19 -- Received: from web34402.mail.mud.yahoo.com (web34402.mail.mud.yahoo.com [66.163.178.151]) 18, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l51EiFbR022834 18, 19 -- for {microscopy-at-sparc5.microscopy.com} ; Fri, 1 Jun 2007 09:44:16 -0500 18, 19 -- Received: (qmail 49157 invoked by uid 60001); 1 Jun 2007 14:44:13 -0000 18, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 18, 19 -- s=s1024; d=yahoo.com; 18, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 18, 19 -- b=ohxTk6rrG9wPMmpEB41srU7p0itpjgVWE9ku/z16opwRMjvNQkPlCv/FugD4wBXDK/YdUZtq8Fe2ynDWSgR08V//oypLZTuy/NjQ0tdAuvFMbqplyM0zeUApL4EskTkDZ7yZHN4XAK7a3eTKe1jsohcONgRn+NKQuOl4xzrnLco=; 18, 19 -- X-YMail-OSG: FtqzHRoVM1mN3BbnjqYQIes90SQ7DzVLUM7AHHe.Y6LKZ5SuJ0ttL5NLQuSwS3nVsIIpObInQHE6fWq_f1ElpwaB0A4nI04bvBUruRmTrj7lGyTjiEzR9j.BWwBTsA-- 18, 19 -- Received: from [141.151.33.213] by web34402.mail.mud.yahoo.com via HTTP; Fri, 01 Jun 2007 07:44:13 PDT 18, 19 -- Date: Fri, 1 Jun 2007 07:44:13 -0700 (PDT) 18, 19 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 18, 19 -- Subject: [Microscopy] RE: viaWWW: Question about image on Glass 18, 19 -- To: microscopy-at-ns.microscopy.com, yitianp-at-ece.arizona.edu 18, 19 -- MIME-Version: 1.0 18, 19 -- Content-Type: text/plain; charset=iso-8859-1 18, 19 -- Content-Transfer-Encoding: 8bit 18, 19 -- Message-ID: {213428.48556.qm-at-web34402.mail.mud.yahoo.com} ==============================End of - Headers==============================
I don't know the particulars of operation of the evaporator unit and my knowledge is for pure gold or platinum (some metals will alloy with the tungsten and cause problems - maybe someone else knows).
Platinum MP = 1772 C Tungsten MP = 3410 C
If the Pt wire is not melting, then the tungsten temperature is too low. If you advance the filament heating slowly (a few seconds for work, but slower in testing until you know the behaviour) you should first see the Pt:Pd wire melt and flow to the bottom of the hairpin (the point of the V is down) and the droplet will evaporate at a slightly higher temperature. Actually you only need to put the wire near the tip of the V.
Just remember to heat the filament over a few seconds, not instantly. Tungsten has a positive temperature coefficient and the filament resistance is initially low and a large surge will occur if full voltage is applied directly - it can cause the evaporant wire to spit or the filament to burn out; but you shouldn't need to be that close to the tungten melting point.
You don't specify distances but it sounds like you are using a lot of wire. You probably only need 10-20Å. 0.5 cm of 8 mil wire at 10 cm distance will give ~13 Å (at normal incidence, or the sides of DNA so disposed....)
Å = (40.3 * diameter^2 * Length) / distance^2
diameter in "mils" (0.001"), 8 mils is a standard size, 0.008" dia length of wire evaporant, distance to sample in cm
I have an Excel spreadsheet with a "calculator" for this formula that I can send if you want - can't go to the Microscopy Listserver..... Just ask.
Hope this helps.
Dale Callaham Univ. of Massachusetts, Amherst
modla-at-dbi.udel.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://ns.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both modla-at-dbi.udel.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: modla-at-dbi.udel.edu } Name: Shannon Modla } } Organization: Delaware Biotechnology Institute } } Title-Subject: [Filtered] Metal Shadow Casting of DNA } } Question: We are currently trying to rotary shadow cast DNA but are having problems obtaining consistent results with the metal shadowing. We are using an 80:20 platinum-palladium wire purchased from EMS and a Denton Bench Top Turbo III sputter coater. The platinum palladium wire was wrapped around a 2-3 cm portion of a hairpin loop formed with tungsten wire. When the filament power is increased, the platinum palladium wire will either heat up without melting and evaporating or else the tungsten wire breaks without appreciable metal shadowing. I was wondering if anyone had any experience with this technique and could offer suggestions about how to obtain more consistent results. } } Thanks, } } Shannon Modla } Delware Biotechnology Institute } BioImaging Center } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Fri Jun 1 07:50:13 2007 } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51CoCCh027317 } 9, 11 -- for {microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 07:50:13 -0500 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240815c285c66f2e87-at-[206.69.208.22]} } 9, 11 -- Date: Fri, 1 Jun 2007 07:50:12 -0500 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: modla-at-dbi.udel.edu (by way of MicroscopyListserver) } 9, 11 -- Subject: viaWWW: Metal Shadow Casting of DNA } 9, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
==============================Original Headers============================== 14, 21 -- From dac-at-research.umass.edu Fri Jun 1 09:55:15 2007 14, 21 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40]) 14, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51EtEr4002073 14, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 09:55:15 -0500 14, 21 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 14, 21 -- (authenticated bits=0) 14, 21 -- by race4.oit.umass.edu (8.13.7/8.13.7) with ESMTP id l51EtCWW031954 14, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 14, 21 -- Fri, 1 Jun 2007 10:55:13 -0400 14, 21 -- Message-ID: {466041E0.4080300-at-research.umass.edu} 14, 21 -- Date: Fri, 01 Jun 2007 10:57:20 -0500 14, 21 -- From: Dale Callaham {dac-at-research.umass.edu} 14, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.2) Gecko/20070222 SeaMonkey/1.1.1 14, 21 -- MIME-Version: 1.0 14, 21 -- To: modla-at-dbi.udel.edu, Microscopy Listserver {Microscopy-at-microscopy.com} 14, 21 -- Subject: Re: [Microscopy] viaWWW: Metal Shadow Casting of DNA 14, 21 -- References: {200706011256.l51Cuoup004737-at-ns.microscopy.com} 14, 21 -- In-Reply-To: {200706011256.l51Cuoup004737-at-ns.microscopy.com} 14, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 21 -- Content-Transfer-Encoding: 8bit 14, 21 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Sorry to occupy your mailbox, but I've had a couple of posts that didn't. Ken
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
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==============================Original Headers============================== 7, 27 -- From kenconverse-at-qualityimages.biz Fri Jun 1 10:14:02 2007 7, 27 -- Received: from dpmailmta02.doteasy.com (dpmailmta02.doteasy.com [65.61.209.90]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51FE2Gt013936 7, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 1 Jun 2007 10:14:02 -0500 7, 27 -- Received: from qualityimages.biz (unverified [192.168.101.16]) 7, 27 -- by dpmailmta02.doteasy.com (DEO) with ESMTP id 80292733-1814644 7, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 01 Jun 2007 10:18:13 -0700 7, 27 -- Received: from Ken [72.227.100.25] by qualityimages.biz with ESMTP 7, 27 -- (SMTPD32-8.05) id A7B342760052; Fri, 01 Jun 2007 08:13:55 -0700 7, 27 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 7, 27 -- To: "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 7, 27 -- Subject: test 7, 27 -- Date: Fri, 1 Jun 2007 11:13:39 -0400 7, 27 -- Message-ID: {001d01c7a45f$71f97250$6401a8c0-at-Ken} 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="us-ascii" 7, 27 -- Content-Transfer-Encoding: 7bit 7, 27 -- X-Priority: 3 (Normal) 7, 27 -- X-MSMail-Priority: Normal 7, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 7, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 7, 27 -- Thread-Index: AcekX262nmEQmKvJQ2OPBpGYnI1zQA== 7, 27 -- Importance: Normal 7, 27 -- X-IMSTrailer: __IMail_7__ 7, 27 -- X-IP-stats: Incoming Last 0, First 246, in=4561928, out=0, spam=0 ip=192.168.101.16 7, 27 -- X-Originating-IP: 192.168.101.16 ==============================End of - Headers==============================
I have recently had a power outage and when I re-started my JEOL 6400 equipped OXFORD INCA X-sight the INCA software gave me an error stating "(1426)DSPP Failed Board" and my deadtime is 99% even with no beam. Can anyone help? Are the two errors related? Thanks Kirk
==============================Original Headers============================== 2, 17 -- From kross-at-laurentian.ca Fri Jun 1 10:15:09 2007 2, 17 -- Received: from lugwout.laurentian.ca (lugwout.laurentian.ca [142.51.1.65]) 2, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51FF9tf015968 2, 17 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 10:15:09 -0500 2, 17 -- Received: from OUTDOM-MTA by lugwout.laurentian.ca 2, 17 -- with Novell_GroupWise; Fri, 01 Jun 2007 11:15:08 -0400 2, 17 -- Message-Id: {465FFF8E0200005200004526-at-lugwout.laurentian.ca} 2, 17 -- X-Mailer: Novell GroupWise Internet Agent 7.0.1 2, 17 -- Date: Fri, 01 Jun 2007 11:14:43 -0400 2, 17 -- From: "Kirk Ross" {kross-at-laurentian.ca} 2, 17 -- To: {Microscopy-at-microscopy.com} 2, 17 -- Subject: Oxford, EDS deadtime 2, 17 -- Mime-Version: 1.0 2, 17 -- Content-Type: text/plain; charset=US-ASCII 2, 17 -- Content-Disposition: inline 2, 17 -- Content-Transfer-Encoding: 8bit 2, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l51FF9tf015968 ==============================End of - Headers==============================
They might be related. However, first thing to do is to home the stage. There should be an option in the SEM control to initialize the stage. Do this. If the stage uses LEDs, this will drive the EDS nuts with high cps and high DT. Restart PC.
If this does not fix the problem, then you might have a damaged board from the power outage.
gary g.
At 07:16 AM 6/1/2007, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Fri Jun 1 11:06:05 2007 10, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l51G64PD012957 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 11:06:05 -0500 10, 20 -- Message-Id: {200706011606.l51G64PD012957-at-ns.microscopy.com} 10, 20 -- Received: (qmail 6663 invoked from network); 1 Jun 2007 08:39:23 -0700 10, 20 -- Received: by simscan 1.1.0 ppid: 6660, pid: 6661, t: 0.0919s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp3 with SMTP; 1 Jun 2007 08:39:23 -0700 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Fri, 01 Jun 2007 08:39:38 -0800 10, 20 -- To: kross-at-laurentian.ca 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] Oxford, EDS deadtime 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200706011516.l51FGYCv021250-at-ns.microscopy.com} 10, 20 -- References: {200706011516.l51FGYCv021250-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-52C91BC8 ==============================End of - Headers==============================
Energy Beam Sciences is the US distributor for Quorum Technologies, which handles both the Polaron, and Emitech line of preparation equipment, including Critical Point Dryers.
Quorum offers both a horizontal and vertical style CPD system, with the horizontal style the user has the advantage of working with small or large samples based on the chamber set-up, with the vertical system offering more of the automatic features.
I'll look to contact you off-line to share details on the systems and your needs.
Regards,
Mike Dufraine Energy Beam Sciences,Inc.
mdienelt-at-msn.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mdienelt-at-msn.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mdienelt-at-msn.com } Name: Margaret Dienelt } } Organization: USDA, ARS, National Arboretum } } Title-Subject: [Filtered] Advice on critical point dryers } } Question: Hello everyone, } } I've just learned we might have funds to replace our ancient, } tempermental critical point dryer and would greatly appreciate any } feedback anyone can give me on their CPD. I'm especially interested } in reliability - the valves in our current unit have been a problem } for years, requiring numerous repairs (usually after destroying a few } samples). } } In addition to information anyone can share on specific models, I } also have two general questions: } } What the pros and cons are to the two profiles, i.e. horizontal (like } Tousimis) and vertical (like Polaron)? } } What are pros and cons to automatic vs manual models? } } Basically, any wisdom you'd like to share about purchasing a new } critical point dryer will be more than welcome. Our new one will be } used in a plant pathology lab and will be used to process primarily } plant tissue. We don't need one for a clean room or one with the } jumbo chamber. } } I'm sending this from my personal e-mail account since I'm having } problems with my government e-mail, but if replies are sent to } mdienelt-at-msn.com or margaret.dienelt-at-ars.usda.gov. I'll receive them } one way or the other. } } Thank you! } } Margaret } } } } Margaret Dienelt } Plant Pathologist/Electron Microscopist } Floral and Nursery Plants Research Unit } National Arboretum, ARS, USDA } } 10300 Baltimore Avenue } Beltsville, MD 20705 } } (301)504-6097 } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 20, 11 -- From zaluzec-at-microscopy.com Thu May 31 15:08:33 2007 } 20, 11 -- Received: from [165.68.138.44] (msdvpn8.msd.anl.gov [130.202.238.72]) } 20, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l4VK8WMU025246 } 20, 11 -- for {microscopy-at-microscopy.com} ; Thu, 31 May 2007 15:08:33 -0500 } 20, 11 -- Mime-Version: 1.0 } 20, 11 -- Message-Id: {p06240800c284dbc68242-at-[206.69.208.26]} } 20, 11 -- Date: Thu, 31 May 2007 15:08:51 -0500 } 20, 11 -- To: microscopy-at-microscopy.com } 20, 11 -- From: mdienelt-at-msn.com (by way of MicroscopyListserver) } 20, 11 -- Subject: viaWWW: Advice on critical point dryers } 20, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
-- Michael F. Dufraine EM - Product Manager TEL 800-992-9037 X340 MDufraine-at-ebsciences.com www.ebsciences.com
==============================Original Headers============================== 9, 26 -- From mdufraine-at-ebsciences.com Fri Jun 1 11:21:41 2007 9, 26 -- Received: from mx-outbound01.easydns.com (mailout.easydns.com [205.210.42.54]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51GLfPS028088 9, 26 -- for {microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 11:21:41 -0500 9, 26 -- Received: from mail.ebsciences.com (unknown [69.182.224.2]) 9, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 9, 26 -- (No client certificate requested) 9, 26 -- by mx-outbound01.easydns.com (Postfix) with ESMTP id A1D3D7FF6; 9, 26 -- Fri, 1 Jun 2007 12:21:39 -0400 (EDT) 9, 26 -- Received: from mdufraine.ebsciences.private ([10.10.0.196]) 9, 26 -- by mail.ebsciences.com with esmtpsa (SSLv3:AES256-SHA:256) 9, 26 -- (Exim 4.67) 9, 26 -- (envelope-from {mdufraine-at-ebsciences.com} ) 9, 26 -- id 1Hu9sh-0004kz-16; Fri, 01 Jun 2007 12:21:39 -0400 9, 26 -- Message-ID: {46604792.4060203-at-ebsciences.com} 9, 26 -- Date: Fri, 01 Jun 2007 12:21:38 -0400 9, 26 -- From: Mike Dufraine {mdufraine-at-ebsciences.com} 9, 26 -- Organization: Energy Beam Sciences 9, 26 -- User-Agent: Thunderbird 2.0.0.0 (Windows/20070326) 9, 26 -- MIME-Version: 1.0 9, 26 -- To: mdienelt-at-msn.com, microscopy-at-microscopy.com 9, 26 -- Subject: Re: [Microscopy] viaWWW: Advice on critical point dryers 9, 26 -- References: {200705312011.l4VKBHYH028552-at-ns.microscopy.com} 9, 26 -- In-Reply-To: {200705312011.l4VKBHYH028552-at-ns.microscopy.com} 9, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
No problem but you bring up a good point. I sent a posting and it didn't show up on the list server. A recent one involved a person in IL. I sent him a direct posting and a dseparate email posting to the list server. Nothing bounced back from either email but the later posting never made it to the list.
I fact, I see replies to postings before I ever see the original posting. I know things get delayed and that delays happen. It's not Nestor's fault. 'It's a server time slicing or sharing thing', IMO. In the next day or two, I might get the original posting. I have no problem there. My problem is that sometimes I never get or will ever receive the original posting (even my own).
So the other day after my posting failed, I sent a short posting to test my ability to still post to the list. I relied to: Re: [Microscopy] Re: AskAMicroscopist: Image Pro Analysis Question - URL with an Image and micrscopy-at-microscopy.com. My reply was posted and my posting received a reply from Mike Bode.
After a week and a half, my first posting still has never showed up.
I can see why some postings come through in double postings. I figure members know this 'lack of posting' problem and so they send double postings.
Paul
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I apologize if I was supposed to post this response to the list. Sometimes I don't see if a message comes from the group or an individual...
dj
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Email: info-at-nomadmet.com Name: Tom Doggart
Organization: Nomad Metallurgy, Inc
Title-Subject: [Filtered] Image Analysis software
Question: I am looking for a user friendly image analysis package to use with my Spot and Nikon cameras and/or their JPEG files. I would like it to do the following at a minimum:
A) Various linear and area measurement B) Phase % calculations for at least 3 phases in a structure C) Image Montague or stitching D) Expanded focus capabilities
Do you have any experience with any system that can do this?
Regardless of which software package you go with, my recommendation for your first step is to save your images as TIFFs!
At 05:47 PM 06/01/07, you wrote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
I use analySIS Opti s/w with Prior E103 Z control to do slice stacking. I don't know if Opti will control your cameras. I use Pixera 600CL and Olympus DP-70 (same driver). Opti controls the Prior stage.
There are several stitching programs. They all have plusses and minusses.
gary g.
At 02:47 PM 6/1/2007, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Fri Jun 1 17:55:26 2007 10, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l51MtQbB027531 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 17:55:26 -0500 10, 20 -- Message-Id: {200706012255.l51MtQbB027531-at-ns.microscopy.com} 10, 20 -- Received: (qmail 10828 invoked from network); 1 Jun 2007 15:55:25 -0700 10, 20 -- Received: by simscan 1.1.0 ppid: 10825, pid: 10826, t: 0.0947s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp4 with SMTP; 1 Jun 2007 15:55:25 -0700 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Fri, 01 Jun 2007 15:55:16 -0800 10, 20 -- To: info-at-nomadmet.com 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] viaWWW: looking for Image Analysis software 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200706012247.l51Mlrs5007769-at-ns.microscopy.com} 10, 20 -- References: {200706012247.l51Mlrs5007769-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-79CE6944 ==============================End of - Headers==============================
-- Shawn Mikula, Ph.D., Postdoctoral Scholar Center for Neuroscience University of California-Davis, 1544 Newton Court, Davis, CA 95618, Phone: 530-754-9209 Fax: 530-754-9136 mail: samikula-at-ucdavis.edu web: http://brainmaps.org
----- Original Message ----- X-from: {info-at-nomadmet.com} To: {samikula-at-ucdavis.edu} Sent: Friday, June 01, 2007 3:53 PM
I have used VIS by Visiopharm. It has incredible segmentation capabilities that are attached to a database. You can build essentially a program to separate an image into the regions of interest and then to all sorts of calculations. This is all done by simple clicking.
----- Original Message ----- X-from: {info-at-nomadmet.com} To: {rboehrin-at-vt.edu} Sent: Friday, June 01, 2007 6:53 PM
Tom
Definitely do not use JPEG. Why spend the money on a quality microscope and quality camera, and then save the image in a bad file-format?
For "expanded (sic extended) focus", you should consider using CombineZ5 (or ZM) and/or Helicon Focus. Each has excellent support and cost.
I would also plug ImageJ and the excellent support/cost.
regards,
Jim
} From mail-at-ns.microscopy.com Fri Jun 1 18:44:41 2007 } Date: Fri, 1 Jun 2007 17:46:48 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: info-at-nomadmet.com } Reply-to: info-at-nomadmet.com } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] viaWWW: looking for Image Analysis software } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both info-at-nomadmet.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: info-at-nomadmet.com } Name: Tom Doggart } } Organization: Nomad Metallurgy, Inc } } Title-Subject: [Filtered] Image Analysis software } } Question: I am looking for a user friendly image analysis package to use with my Spot and Nikon cameras and/or their JPEG files. I would like it to do the following at a minimum: } } } } A) Various linear and area measurement } B) Phase % calculations for at least 3 phases in a structure } C) Image Montague or stitching } D) Expanded focus capabilities } } } } Do you have any experience with any system that can do this? } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 11 -- From zaluzec-at-microscopy.com Fri Jun 1 17:45:11 2007 } 11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l51MjA7f004949 } 11, 11 -- for {microscopy-at-microscopy.com} ; Fri, 1 Jun 2007 17:45:10 -0500 } 11, 11 -- Mime-Version: 1.0 } 11, 11 -- Message-Id: {p06240800c28651b9d48b-at-[206.69.208.22]} } 11, 11 -- Date: Fri, 1 Jun 2007 17:45:09 -0500 } 11, 11 -- To: microscopy-at-microscopy.com } 11, 11 -- From: info-at-nomadmet.com (by way of MicroscopyListserver) } 11, 11 -- Subject: viaWWW: looking for Image Analysis software } 11, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 12 -- From jquinn-at-www.matscieng.sunysb.edu Sat Jun 2 11:51:44 2007 9, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 9, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l52GpiPe017688 9, 12 -- for {microscopy-at-microscopy.com} ; Sat, 2 Jun 2007 11:51:44 -0500 9, 12 -- Received: (from jquinn-at-localhost) 9, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id l52GnOh19572; 9, 12 -- Sat, 2 Jun 2007 12:49:24 -0400 9, 12 -- Date: Sat, 2 Jun 2007 12:49:24 -0400 9, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 9, 12 -- Message-Id: {200706021649.l52GnOh19572-at-www.matscieng.sunysb.edu} 9, 12 -- To: info-at-nomadmet.com, microscopy-at-microscopy.com 9, 12 -- Subject: Re: [Microscopy] viaWWW: looking for Image Analysis software ==============================End of - Headers==============================
If the DNA is already attached to a substrate, I'd really suggest that you try AFM instead. It will give you essentially atomic level resolution, without any disruptive coating.
Contact me off line if you need more info.
Thanks Barbara
At 12:12 AM 6/4/2007, dac-at-research.umass.edu wrote:
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==============================Original Headers============================== 11, 20 -- From bfoster-at-mme1.com Mon Jun 4 00:17:14 2007 11, 20 -- Received: from smtp109.sbc.mail.re2.yahoo.com (smtp109.sbc.mail.re2.yahoo.com [68.142.229.96]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l545HDLO017834 11, 20 -- for {microscopy-at-microscopy.com} ; Mon, 4 Jun 2007 00:17:14 -0500 11, 20 -- Message-Id: {200706040517.l545HDLO017834-at-ns.microscopy.com} 11, 20 -- Received: (qmail 95349 invoked from network); 4 Jun 2007 05:17:13 -0000 11, 20 -- Received: from unknown (HELO barbsd505.mme1.com) (bfostermme-at-sbcglobal.net-at-68.94.37.215 with login) 11, 20 -- by smtp109.sbc.mail.re2.yahoo.com with SMTP; 4 Jun 2007 05:17:13 -0000 11, 20 -- X-YMail-OSG: PpL5dW0VM1kt8KYnIVL4g1oZMUp4.mahfGdZvNfji8X3zsfIuVsrQrnVOiJq9Kv9LV7U_YndJVnWx5tToDmY496aw_dkSDJumIHrq99QK17oZO2lsNRydtQNUdeEZW3hzCHYDc5ggnkDLuyycuDuyIwY17l8iDrk53o2k_OQFOY- 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Mon, 04 Jun 2007 00:16:37 -0500 11, 20 -- To: dac-at-research.umass.edu, microscopy-at-microscopy.com 11, 20 -- From: Barbara Foster {bfoster-at-mme1.com} 11, 20 -- Subject: Re: [Microscopy] Re: viaWWW: Metal Shadow Casting of DNA 11, 20 -- In-Reply-To: {200706011457.l51Evc6o005808-at-ns.microscopy.com} 11, 20 -- References: {200706011457.l51Evc6o005808-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 11, 20 -- Content-Transfer-Encoding: 8bit 11, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l545HDLO017834 ==============================End of - Headers==============================
Dale Callaham wrote: ================================================================== } I don't know the particulars of operation of the evaporator unit and my } knowledge is for pure gold or platinum (some metals will alloy with the } tungsten and cause problems - maybe someone else knows). } } Platinum MP = 1772 C } Tungsten MP = 3410 C } } If the Pt wire is not melting, then the tungsten temperature is too low. } If you advance the filament heating slowly (a few seconds for work, but } slower in testing until you know the behaviour) you should first see the } Pt:Pd wire melt and flow to the bottom of the hairpin (the point of the } V is down) and the droplet will evaporate at a slightly higher } temperature. Actually you only need to put the wire near the tip of the V. } } Just remember to heat the filament over a few seconds, not instantly. } Tungsten has a positive temperature coefficient and the filament } resistance is initially low and a large surge will occur if full voltage } is applied directly - it can cause the evaporant wire to spit or the } filament to burn out; but you shouldn't need to be that close to the } tungten melting point. } } You don't specify distances but it sounds like you are using a lot of } wire. You probably only need 10-20Å. 0.5 cm of 8 mil wire at 10 cm } distance will give ~13 Å (at normal incidence, or the sides of DNA so } disposed....) } } Å = (40.3 * diameter^2 * Length) / distance^2 } } diameter in "mils" (0.001"), 8 mils is a standard size, 0.008" dia } length of wire evaporant, distance to sample in cm ================================================================= I have always thought that the best way to "shadow" was to use the "Pt/C sesile drop" method. I learned about it many years ago in graduate school and don't know who used it originally. But one gets a finer grain if Pt/C is evaporated simultaneously than if Pt alone is evaporated. The way to do this is to take a carbon rod that has been sharpened down to a "neck", wrap about the "neck" not more than 30-40 mm of 8 mil Pt wire, put the bell jar onto the vacuum evaporator but don't pump down. Slowly increase the current through the carbon rods to the point where, just beyond cherry red, the Pt melts and because of surface tension effects, and the fact that liquid Pt does not want to wet carbon, it "beads up", just as water does on a freshly waxed car. Once this happens, turn off the current, and when it cools down, rotate the bead so that it is facing the surface to be shadowed.
Then the bell jar is put in place, the chamber pumped down, and the carbon rod is evaporated but what really comes off is a combination of Pt and C.
Note: The optimum amount of wire to be used depends on a)distance to the substrate to be coated and b) shadowing angle.
Since Pt wants to alloy readily with W, this approach avoids all those other issues as well.
Disclaimer: SPI Supplies offers on our website Pt wire and presharpened carbon rods so we would have a vested interest in seeing this technique used more rather than less frequently.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: http://www.2spi.com ############################ ==================================================
==============================Original Headers============================== 9, 20 -- From cgarber-at-2spi.com Mon Jun 4 00:59:12 2007 9, 20 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l545xCIY029855 9, 20 -- for {microscopy-at-msa.microscopy.com} ; Mon, 4 Jun 2007 00:59:12 -0500 9, 20 -- Received: from [192.168.1.101] (c-71-230-36-118.hsd1.pa.comcast.net [71.230.36.118]) 9, 20 -- (authenticated bits=0) 9, 20 -- by diskless5.axs2000.net (8.12.11/8.12.11) with ESMTP id l545xBSh019678 9, 20 -- for {microscopy-at-msa.microscopy.com} ; Mon, 4 Jun 2007 01:59:12 -0400 9, 20 -- X-IDV-FirstRcvd: c-71-230-36-118.hsd1.pa.comcast.net [71.230.36.118] 9, 20 -- X-IDV-HELO: [192.168.1.101] 9, 20 -- X-IDV-Authenticated-User: cgarber 9, 20 -- Message-ID: {4663AA2F.7060406-at-2spi.com} 9, 20 -- Date: Mon, 04 Jun 2007 01:59:11 -0400 9, 20 -- From: "Garber, Charles A" {cgarber-at-2spi.com} 9, 20 -- User-Agent: Thunderbird 2.0.0.0 (Windows/20070326) 9, 20 -- MIME-Version: 1.0 9, 20 -- To: Microscopy Listserver {microscopy-at-msa.microscopy.com} 9, 20 -- Subject: Shadowing with Pt for TEM 9, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I second Barbara Foster's suggestion. For an example of DNA imaging by AFM, see our website: http://www.asmicro.com/Applications/DNA_Molecules.htm
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] ============================================= ----- Original Message ----- From: bfoster-at-mme1.com To: donc-at-asmicro.com Sent: Monday, June 04, 2007 1:21 AM Subject: [a] [Microscopy] viaWWW: Metal Shadow Casting of DNA
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Sharon,
If the DNA is already attached to a substrate, I'd really suggest that you try AFM instead. It will give you essentially atomic level resolution, without any disruptive coating.
Contact me off line if you need more info.
Thanks Barbara
At 12:12 AM 6/4/2007, dac-at-research.umass.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Shannon, } } I don't know the particulars of operation of the evaporator unit and my } knowledge is for pure gold or platinum (some metals will alloy with the } tungsten and cause problems - maybe someone else knows). } } Platinum MP = 1772 C } Tungsten MP = 3410 C } } If the Pt wire is not melting, then the tungsten temperature is too low. } If you advance the filament heating slowly (a few seconds for work, but } slower in testing until you know the behaviour) you should first see the } Pt:Pd wire melt and flow to the bottom of the hairpin (the point of the } V is down) and the droplet will evaporate at a slightly higher } temperature. Actually you only need to put the wire near the tip of the V. } } Just remember to heat the filament over a few seconds, not instantly. } Tungsten has a positive temperature coefficient and the filament } resistance is initially low and a large surge will occur if full voltage } is applied directly - it can cause the evaporant wire to spit or the } filament to burn out; but you shouldn't need to be that close to the } tungten melting point. } } You don't specify distances but it sounds like you are using a lot of } wire. You probably only need 10-20Å. 0.5 cm of 8 mil wire at 10 cm } distance will give ~13 Å (at normal incidence, or the sides of DNA so } disposed....) } } Å = (40.3 * diameter^2 * Length) / distance^2 } } diameter in "mils" (0.001"), 8 mils is a standard size, 0.008" dia } length of wire evaporant, distance to sample in cm } } I have an Excel spreadsheet with a "calculator" for this formula that I } can send if you want - can't go to the Microscopy Listserver..... Just ask. } } Hope this helps. } } Dale Callaham } Univ. of Massachusetts, Amherst } } } modla-at-dbi.udel.edu wrote: } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at http://ns.microscopy.com/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from } a Subscriber, so when replying } } please copy both modla-at-dbi.udel.edu as well } as the MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: modla-at-dbi.udel.edu } } Name: Shannon Modla } } } } Organization: Delaware Biotechnology Institute } } } } Title-Subject: [Filtered] Metal Shadow Casting of DNA } } } } Question: We are currently trying to rotary } shadow cast DNA but are having problems } obtaining consistent results with the metal shadowing. We are using an } 80:20 } platinum-palladium wire purchased from EMS and a Denton Bench Top Turbo } III sputter coater. } The platinum palladium wire was wrapped around a 2-3 cm portion of a } hairpin loop formed } with tungsten wire. When the filament power is increased, the platinum } palladium wire will } either heat up without melting and evaporating or else the tungsten wire } breaks without } appreciable metal shadowing. I was wondering if anyone had any } experience with } this technique and could offer suggestions about how to obtain more } consistent results. } } } } Thanks, } } } } Shannon Modla } } Delware Biotechnology Institute } } BioImaging Center } } } } } } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== ... } } 9, 11 -- Date: Fri, 1 Jun 2007 07:50:12 -0500 } } 9, 11 -- To: microscopy-at-microscopy.com } } 9, 11 -- From: modla-at-dbi.udel.edu (by way of MicroscopyListserver) } } 9, 11 -- Subject: viaWWW: Metal Shadow Casting of DNA } } 9, 11 -- Content-Type: text/plain; charset="us-ascii" } } ==============================End of - } Headers============================== ... } 14, 21 -- From dac-at-research.umass.edu Fri Jun 1 09:55:15 2007 } 14, 21 -- Received: from race4.oit.umass.edu } (race4.oit.umass.edu [128.119.101.40]) } 14, 21 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id l51EtEr4002073 } 14, 21 -- for {Microscopy-at-microscopy.com} ; } Fri, 1 Jun 2007 09:55:15 -0500
==============================Original Headers============================== 17, 24 -- From donc-at-asmicro.com Mon Jun 4 08:29:44 2007 17, 24 -- Received: from smtp111.sbc.mail.re2.yahoo.com (smtp111.sbc.mail.re2.yahoo.com [68.142.229.94]) 17, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l54DThYA018354 17, 24 -- for {microscopy-at-microscopy.com} ; Mon, 4 Jun 2007 08:29:43 -0500 17, 24 -- Received: (qmail 37684 invoked from network); 4 Jun 2007 13:29:43 -0000 17, 24 -- Received: from unknown (HELO asm15) (asmicro-at-sbcglobal.net-at-68.23.9.238 with login) 17, 24 -- by smtp111.sbc.mail.re2.yahoo.com with SMTP; 4 Jun 2007 13:29:42 -0000 17, 24 -- X-YMail-OSG: wxcJFFoVM1nYRj0FMOh83REceMi8qLEX6NzzIJNqiB1Zrg5FCx4MaG0i5XzsoOmR8Khn9DEZ5_g.l5U1wmjsRbBh_GGZ57_XVKpUgtMYNRCeM10OVvOpYixngy8IiOceIe7n0hIJDI6JEKqEKC65krTscifmimglTYUwNgcexDwQ 17, 24 -- Message-ID: {001601c7a6ac$6858e330$6901a8c0-at-asm15} 17, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 17, 24 -- To: {bfoster-at-mme1.com} , "Microscopy List" {microscopy-at-microscopy.com} 17, 24 -- References: {200706040521.l545LZE3022955-at-ns.microscopy.com} 17, 24 -- Subject: Re: [a] [Microscopy] viaWWW: Metal Shadow Casting of DNA 17, 24 -- Date: Mon, 4 Jun 2007 09:29:28 -0400 17, 24 -- MIME-Version: 1.0 17, 24 -- Content-Type: text/plain; 17, 24 -- format=flowed; 17, 24 -- charset="iso-8859-1"; 17, 24 -- reply-type=original 17, 24 -- Content-Transfer-Encoding: 8bit 17, 24 -- X-Priority: 3 17, 24 -- X-MSMail-Priority: Normal 17, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3028 17, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 ==============================End of - Headers==============================
I'm reading Falk, Brill and Stork's "Seeing the Light: Optics in Nature, Photography, Color, Vision, and Holography." I like the book, in fact love it, but I want something more dedicated to the physics/math. I did order the Schaum's outline for problems.
Can someone recommend a good undergraduate level optics text? My college level math and physics are fine.
Has anyone used Eugene Hecht's Optics either as a student or instructor for a course? Is it good enough to actually buy, or are there better texts?
Thanks,
K. Leo Pullin
==============================Original Headers============================== 1, 19 -- From kleopullin-at-pacbell.net Mon Jun 4 14:48:56 2007 1, 19 -- Received: from web83411.mail.sp1.yahoo.com (web83411.mail.sp1.yahoo.com [69.147.64.59]) 1, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l54JmunN008288 1, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 4 Jun 2007 14:48:56 -0500 1, 19 -- Received: (qmail 89759 invoked by uid 60001); 4 Jun 2007 19:26:11 -0000 1, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 1, 19 -- s=s1024; d=pacbell.net; 1, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 1, 19 -- b=0vM1D/7vc57qgV0I82v2GjAnaog0bpzR064ixJcjfMRp+odYURE3SWEI40ed4D6dSaMwww+0zmTSYocX+ioapkWXoTXjXwpqIiX2fAUboN8J0U5n+bCtSufgihHWet5997UjlnyX3PMXCrhchvwy007ckDHqanz5/7LMBdeCPIs=; 1, 19 -- X-YMail-OSG: oeCbtN0VM1nO4bG5RWWk8gesgyEkUmyg83tp5cPuAe6Fw.uV 1, 19 -- Received: from [69.226.100.188] by web83411.mail.sp1.yahoo.com via HTTP; Mon, 04 Jun 2007 12:26:11 PDT 1, 19 -- Date: Mon, 4 Jun 2007 12:26:11 -0700 (PDT) 1, 19 -- From: Kleo Pullin {kleopullin-at-pacbell.net} 1, 19 -- Subject: LM Optical physics text -- recommendations? 1, 19 -- To: Microscopy-at-microscopy.com 1, 19 -- MIME-Version: 1.0 1, 19 -- Content-Type: text/plain; charset=iso-8859-1 1, 19 -- Content-Transfer-Encoding: 8bit 1, 19 -- Message-ID: {670379.83959.qm-at-web83411.mail.sp1.yahoo.com} ==============================End of - Headers==============================
ImageJ is free and you get what you paid for. I do not know whether it will control the Prior stage controllers. If it does, great. Problem solved.
However, if not, then you need industrial strength software like analySIS--or current generation. This controls the Z axis stepper and automatically collects Z stacks and then assembles them into a high depth of focus final image. I typically do 20 slices. OK....do this yourself manually and try to get a good result. I did and after beating my head against the wall too many times, it was obvious that another method was necessary.
The focus assembly method is based on contrast. So many small increments of Z are ideal. analySIS and the Prior stage controller are seamlessly integrated.
gary g.
At 03:04 PM 6/1/2007, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 10, 21 -- From gary-at-gaugler.com Mon Jun 4 15:25:10 2007 10, 21 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l54KPASn020921 10, 21 -- for {microscopy-at-microscopy.com} ; Mon, 4 Jun 2007 15:25:10 -0500 10, 21 -- Message-Id: {200706042025.l54KPASn020921-at-ns.microscopy.com} 10, 21 -- Received: (qmail 29863 invoked from network); 4 Jun 2007 13:25:09 -0700 10, 21 -- Received: by simscan 1.1.0 ppid: 29860, pid: 29861, t: 0.1494s 10, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 21 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 21 -- by qsmtp1 with SMTP; 4 Jun 2007 13:25:09 -0700 10, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 21 -- Date: Mon, 04 Jun 2007 13:24:10 -0800 10, 21 -- To: samikula-at-ucdavis.edu 10, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 21 -- Subject: Re: [Microscopy] Re: viaWWW: looking for Image Analysis 10, 21 -- software 10, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 21 -- In-Reply-To: {200706012304.l51N4xbP009673-at-ns.microscopy.com} 10, 21 -- References: {200706012304.l51N4xbP009673-at-ns.microscopy.com} 10, 21 -- Mime-Version: 1.0 10, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-7C5A6774 ==============================End of - Headers==============================
Is there anybody here who has used the SPI Combined Sputter and Carbon Coater System? Any feedback? For those who have combined systems, have you encountered any contamination issues? How can this best be avoided?
Aside from good sputtering/coating capability, we are also considering having a dual system with smaller footprint since we have a very crowded lab. Any brand/model you can suggest?
Thank you so much.
Melina Miralles Lab Technician The Petroleum Institute Abu Dhabi, UAE
==============================Original Headers============================== 7, 27 -- From mmiralles-at-pi.ac.ae Tue Jun 5 00:06:07 2007 7, 27 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l555651V010495 7, 27 -- for {microscopy-at-microscopy.com} ; Tue, 5 Jun 2007 00:06:07 -0500 7, 27 -- Received: from pi-exf.pi.ac.ae ([192.168.2.11]) 7, 27 -- by mx1.pi.ac.ae with ESMTP; 05 Jun 2007 09:10:17 +0400 7, 27 -- X-IronPort-AV: i="4.16,383,1175457600"; 7, 27 -- d="scan'208"; a="1648798:sNHT24529136" 7, 27 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.1830); 7, 27 -- Tue, 5 Jun 2007 09:05:58 +0400 7, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 27 -- Content-class: urn:content-classes:message 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="windows-1256" 7, 27 -- Subject: Sputter & Carbon Coater 7, 27 -- Date: Tue, 5 Jun 2007 09:05:56 +0400 7, 27 -- Message-ID: {D5603421C6303A46883F87E7968F285B02DFEA31-at-pi-exm.PI.AC.AE} 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- Thread-Topic: Sputter & Carbon Coater 7, 27 -- Thread-Index: AcenLzLfFcyEFZDRQJiG1jBHFpDM3Q== 7, 27 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae} 7, 27 -- To: {microscopy-at-microscopy.com} 7, 27 -- X-OriginalArrivalTime: 05 Jun 2007 05:05:58.0273 (UTC) FILETIME=[34196710:01C7A72F] 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l555651V010495 ==============================End of - Headers==============================
Dr Peters gives me permission today by email to quote on the LisServer her clarification/correction, sent to me earlier today, that only UrAc, but not Pb Citrate, was used as a block stain in her work. Personally, I am quite grateful we were briefly misled, because it brought in David Elliott's response on his use of lead acetate block stain (in EtOH-acetone 50:50) following aqueous UrAc.
Here is Dr Peter's clarification/correction: "Our method is as follows: We use uranyl acetate in 70% ethanol as a block stain ....... then [after] the tissue is embedded in Epon and polymerized..... We use the lead citrate only for the ready prepared sections. I am sorry if my phrasing [turned out to] be kind of misleading."
I plan continued exploration of both lead salts. Use as a block stain in organic solvent after UrAc especially interests me for freeze-substitution. So far, I find both are insoluble or nearly so in 100% acetone, so the EtOH-containing vehicle in Elliott's procedure seems necessary. Does anyone know or remember if the extreme alkalinity of aqueous lead citrate section stains is essential for releasing the Pb from the strongly sequestering citrate? I'm not sure that pH 11 can be approached in pure organic solvent, but why not try....?
I would guess that prolonging the exposure of tissue blocks to organic solvent for extended block-staining is NOT likely to cause additional shrinkage or extraction of cells, because I think the binding of metals tends to stabilizes the components. ---not completely against shrinkage however; I find that freeze-substitution in acetone-TA followed by acetone UrAc fails to prevent variable amounts of shrinkage, 5-12%, of the myofilament lattice spacing in striated muscle; I'd like someday to see the process monitored by x-ray cryo-diffraction to identify the stage where this occurs and seek ways to prevent or minimize the shrinkage.
-mike reedy-
*************************** At 10:07 PM -0500 5/27/07, dianavd-at-eye.usyd.edu.au wrote: ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Geoff McA asked for the reference to the paper I mentioned. It's Peters S et al; American Journal of Ophthalmology (2007) 143:995-1002; Ultrastructural findings in the primate eye after intravitreal injection of Bevacizumab. All it says is
"postfixed with 1% OsO4 at room temperature in 0.1 M cacodylate buffer (pH 7.4) for three hours, bloc-stained with uranyl acetate and lead citrate, and embedded in Epon after dehydration in a graded series of acetones"
I've Emailed asking for more details. I have the PDF if anyone would like to see the pics.
Diana
} ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } I've just read a paper where the author talks about block staining } with uranyl acetate and lead citrate. UAc yes, but Pbcitrate? Has } anyone heard of this? Does it work? The pictures in the paper were } very nice; good contrast and detail. With the recent talk about } general lack of contrast in specimens these days (I quite agree on } that; I find contrast poorer now than in the past), could this be } a new method? } } All opinions please! } } Cheers, } } Diana }
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
For those of you following the use of lead citrate as a block stain discussion, I had a reply from the author of the paper where the method was described. It turns out to have been incorrect; PbC was not used as a block stain. It was an English usage problem - the author is German - she actually used the PbC in the usual way as a section stain. Still, the idea got us here on the List thinking!
Cheers,
Diana
==============================Original Headers============================== 4, 18 -- From dianavd-at-eye.usyd.edu.au Tue Jun 5 19:23:45 2007 4, 18 -- Received: from galen.med.usyd.edu.au (nugalen.med.usyd.edu.au [129.78.36.39]) 4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l560NiMh031539 4, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 5 Jun 2007 19:23:45 -0500 4, 18 -- Received: from [129.78.203.144] (helo=[129.78.203.144]) 4, 18 -- by galen.med.usyd.edu.au with esmtp (Exim 4.43) 4, 18 -- id 1HvjJO-0006Mg-QC 4, 18 -- for Microscopy-at-microscopy.com; Wed, 06 Jun 2007 10:23:42 +1000 4, 18 -- Mime-Version: 1.0 (Apple Message framework v752.2) 4, 18 -- Content-Transfer-Encoding: 7bit 4, 18 -- Message-Id: {0CF1515D-7F8A-490C-B62F-2FE8CFF0C98F-at-eye.usyd.edu.au} 4, 18 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 4, 18 -- To: Microscopy {Microscopy-at-microscopy.com} 4, 18 -- From: Diana van Driel {dianavd-at-eye.usyd.edu.au} 4, 18 -- Subject: Pb citrate block staining - followup 4, 18 -- Date: Wed, 6 Jun 2007 10:23:37 +1000 4, 18 -- X-Mailer: Apple Mail (2.752.2) 4, 18 -- X-Spam-Score: -4.4 (----) ==============================End of - Headers==============================
Hi - We have an old Electroscan which has proved to be fairly reliable over the years, but lately it has been having problems. Recently, the CPU board died and we were fortunate enough to have a spare (we basically have a spare for everything with this machine). This spare worked in that I could communicate with the CPU via its RS232 port and have full access to its command set. With the self test the CPU board says its OK. Unfortunately, we now seem to have no image coming through. I've played with the command set menu hoping to get an image. At one point I was able to see an extremely weak signal but I'm not sure how I really achieved it simply because there are so many different command options. What I want is Volume 6 - APPENDICES, from the ElectroScan manual set (we have the others). This manual goes into depth about the system software structure. What I also want, if possible, is the service-engineer manual which would detail the actual initial setup of the E3, and any advice in regard to my E3 problem is really welcome.
Thanks
Peter Duncan Senior Technician
==============================Original Headers============================== 4, 30 -- From pcduncan-at-cyllene.uwa.edu.au Tue Jun 5 20:41:03 2007 4, 30 -- Received: from asclepius2.uwa.edu.au (asclepius2.uwa.edu.au [130.95.128.59]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l561f2c1011618 4, 30 -- for {Microscopy-at-Microscopy.Com} ; Tue, 5 Jun 2007 20:41:03 -0500 4, 30 -- Received: from panacea.kas (localhost.localdomain [127.0.0.1]) 4, 30 -- by panacea.uwa.edu.au (Postfix) with SMTP id CAC4988A14 4, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 6 Jun 2007 09:41:01 +0800 (WST) 4, 30 -- Received: from panacea (localhost.localdomain [127.0.0.1]) 4, 30 -- by panacea.prekas (Postfix) with ESMTP id 53DFF88961 4, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 6 Jun 2007 09:41:01 +0800 (WST) 4, 30 -- X-UWA-Client-IP: 130.95.124.134 (UWA) 4, 30 -- Received: from [130.95.124.134] (tearm.cmm.uwa.edu.au [130.95.124.134]) 4, 30 -- by panacea.input (Postfix) with ESMTP id A5EA4889F8 4, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 6 Jun 2007 09:41:00 +0800 (WST) 4, 30 -- Mime-Version: 1.0 (Apple Message framework v624) 4, 30 -- Content-Transfer-Encoding: 7bit 4, 30 -- Message-Id: {1ecd241b5a6eb5723598b05d0f41da01-at-cyllene.uwa.edu.au} 4, 30 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 30 -- To: Microscopy-at-Microscopy.Com 4, 30 -- From: Peter Duncan {pcduncan-at-cyllene.uwa.edu.au} 4, 30 -- Subject: SEM - Electroscan E3 problem - require service engineer manual for initial setup procedure. 4, 30 -- Date: Wed, 6 Jun 2007 09:43:37 +0800 4, 30 -- X-Mailer: Apple Mail (2.624) 4, 30 -- X-Anti-Virus: Kaspersky Anti-Virus for MailServers 5.5.10/RELEASE, bases: 06062007 #318960, status: clean 4, 30 -- X-SpamTest-Info: Profile: Formal (1243/070604) 4, 30 -- X-SpamTest-Info: Profile: Detect Hard [UCS 2006-10-25] 4, 30 -- X-SpamTest-Info: Profile: SysLog 4, 30 -- X-SpamTest-Info: Profile: Marking Spam - Subject (UCS) [2006-10-25] 4, 30 -- X-SpamTest-Status: Not detected 4, 30 -- X-SpamTest-Version: SMTP-Filter Version 2.0.0 [0125], KAS/Release ==============================End of - Headers==============================
Some days ago there was a question posed about the educational benefits of table top SEM and nano-scale samples. In a few weeks I'll post my experiences using a Hitachi TM-1000 table top SEM in a Nanotechnology course for high schoolers as part of the Duke University Talent Identification Program (TIP). More about the program can be found at 'www.tip.duke.edu'. I've chosen the TM-1000 to introduce the students to the micro and nanoscale, and we will be using the SEM, in addition to AFM and HRTEM to characterize Au and Ag nanoparticles the students synthesize as part of a experiential learning activity.
In my opinion the table top SEM will allow the students hands-on experience with an electron microscope. Not that it will resolve atomic columns in nanoparticles, but as a way to introduce them to the scale of things. The intention is to let the students choose ANY samples they are interested in and let them discover the detail of features on the micro and nanoscale.
If anyone has had experience integrating these microscopies into a high school course on nanotechnology I would be most interested in learning more about how it was accomplished and the outcomes. I would also be more than happy to share the syllabus created for the course if requested.
Stay tuned, I'll post the student's data and feedback on the table top SEM, AFM and TEM.
Donovan
==============================Original Headers============================== 13, 28 -- From donovan.leonard-at-gmail.com Tue Jun 5 22:13:59 2007 13, 28 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.226]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l563DxNI024420 13, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 5 Jun 2007 22:13:59 -0500 13, 28 -- Received: by wx-out-0506.google.com with SMTP id s14so7628wxc 13, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 05 Jun 2007 20:13:58 -0700 (PDT) 13, 28 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 13, 28 -- d=gmail.com; s=beta; 13, 28 -- h=domainkey-signature:received:received:message-id:date:from:user-agent:mime-version:to:subject:content-type:content-transfer-encoding; 13, 28 -- b=dyfVrH1XBQkmwdsDufL4zkQVNizVyorQxOguKO1Y1BAksaT8760tGnsprMsuG43dTxEh3irILCXBg/kzxQj4AKaZKQMhTcqENG4Rkm7waREJgOgZE1SKysd+D2gmkenYL+uUcRAm+xdcfdmCiNHq2v5NB24SRXOqyRLK8i9jhoM= 13, 28 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 13, 28 -- d=gmail.com; s=beta; 13, 28 -- h=received:message-id:date:from:user-agent:mime-version:to:subject:content-type:content-transfer-encoding; 13, 28 -- b=FEhNU7orpaaLyXrz/KH/TSVRGLUGh1JI85MEocNjmPB0aOMRisv7LjWEXjIXXMdgX/L/3+Pi5wBcFMvmawex38VOjSHhS1XTV4LB/1/cDxrmbC0gLfENbDR3RdgvogTDZ+DWU2atIamfmAktj2IMTYN+z5bx9APD/RVY3RUTMvY= 13, 28 -- Received: by 10.70.87.5 with SMTP id k5mr113569wxb.1181099638766; 13, 28 -- Tue, 05 Jun 2007 20:13:58 -0700 (PDT) 13, 28 -- Received: from ?192.168.1.95? ( [216.77.224.100]) 13, 28 -- by mx.google.com with ESMTP id i37sm3549106wxd.2007.06.05.20.13.57; 13, 28 -- Tue, 05 Jun 2007 20:13:57 -0700 (PDT) 13, 28 -- Message-ID: {4666266E.5060904-at-gmail.com} 13, 28 -- Date: Tue, 05 Jun 2007 23:13:50 -0400 13, 28 -- From: DNL {donovan.leonard-at-gmail.com} 13, 28 -- User-Agent: Thunderbird 1.5.0.12 (Windows/20070509) 13, 28 -- MIME-Version: 1.0 13, 28 -- To: Microscopy-at-microscopy.com 13, 28 -- Subject: Table Top SEM & High School Nano Course 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have helped get a SEM put into my local high school. The science teacher there now is able to offer 8 college credits to students that take his advanced program related to this instrument. Mark would be able to talk to you more about that aspect and how he integrates it into his curriculum. I have cc'd him with this email so you'll have his email address. This is still in the beginning stages so there is a lot yet to know and learn. But I think it is an absolutely fabulous opportunity for learning...
In the process of getting the SEM into my local high school, I have learned a fair amount about SEM usage in high schools in the US and a bit internationally. There are pockets of high schools around the USA that have this kind of technology operating in high schools, but not a lot. Germany has some high schools using it, apparently Japan has it extensively used throughout their high school system.
You are using Au and Ag particles. My experience indicates high school students seem more interested in biological samples rather than materials. It would be very useful if a materials curriculum could be developed that would interest students at this level.
Finding teachers and school systems that are open to this and have the science background to make it work well, I think is a large hurdle. If a solid curriculm could be put together that would work as a framework for teachers to use, I think that would be very helpful.
Schools are, justifiably, reluctant to use this technology as maintaining these machines is quite expensive and most schools run on very tight budgets. In the cases where I have seen SEM's working in high schools, there has usually been someone that is knowledgable about the instruments and can maintain them for the school pro-bono. These individuals have been key in getting SEM's into high schools. (There may be a program in western PA that's an exception to this, but I've not had any luck contacting them.)
Do send me your curriculum and further information about what you are doing. I can give you more details of programs I've discovered if you wish, but I don't think those details are of general interest to the list (do correct me if I'm wrong).
dj
On Tue, 5 Jun 2007, donovan.leonard-at-gmail.com wrote:
} Hello Listsers - } } Some days ago there was a question posed about the educational benefits } of table top SEM and nano-scale samples. In a few weeks I'll post my } experiences using a Hitachi TM-1000 table top SEM in a Nanotechnology } course for high schoolers as part of the Duke University Talent } Identification Program (TIP). More about the program can be found at } 'www.tip.duke.edu'. I've chosen the TM-1000 to introduce the students } to the micro and nanoscale, and we will be using the SEM, in addition to } AFM and HRTEM to characterize Au and Ag nanoparticles the students } synthesize as part of a experiential learning activity. } } In my opinion the table top SEM will allow the students hands-on } experience with an electron microscope. Not that it will resolve atomic } columns in nanoparticles, but as a way to introduce them to the scale of } things. The intention is to let the students choose ANY samples they } are interested in and let them discover the detail of features on the } micro and nanoscale. } } If anyone has had experience integrating these microscopies into a high } school course on nanotechnology I would be most interested in learning } more about how it was accomplished and the outcomes. I would also be } more than happy to share the syllabus created for the course if requested. } } Stay tuned, I'll post the student's data and feedback on the table top } SEM, AFM and TEM. } } Donovan
==============================Original Headers============================== 11, 19 -- From dljones-at-bestweb.net Wed Jun 6 08:07:58 2007 11, 19 -- Received: from vms044pub.verizon.net (vms044pub.verizon.net [206.46.252.44]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56D7wjs014386 11, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 08:07:58 -0500 11, 19 -- Received: from localhost ([71.247.229.59]) 11, 19 -- by vms044.mailsrvcs.net (Sun Java System Messaging Server 6.2-6.01 (built Apr 11, 19 -- 3 2006)) with ESMTPA id {0JJ7000MBTSK7117-at-vms044.mailsrvcs.net} for 11, 19 -- Microscopy-at-microscopy.com; Wed, 06 Jun 2007 08:07:44 -0500 (CDT) 11, 19 -- Date: Wed, 06 Jun 2007 09:08:42 -0400 (Eastern Daylight Time) 11, 19 -- From: "David L. Jones" {dljones-at-bestweb.net} 11, 19 -- Subject: Re: [Microscopy] Table Top SEM & High School Nano Course 11, 19 -- In-reply-to: {200706060317.l563H7XP030532-at-ns.microscopy.com} 11, 19 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 11, 19 -- To: donovan.leonard-at-gmail.com 11, 19 -- Cc: Microscopy-at-microscopy.com, Mark Patinella {mpatinel-at-haldane.lhric.org} 11, 19 -- Message-id: {Pine.WNT.4.64.0706060825240.3076-at-H-F1} 11, 19 -- MIME-version: 1.0 11, 19 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 11, 19 -- References: {200706060317.l563H7XP030532-at-ns.microscopy.com} ==============================End of - Headers==============================
We unexpectedly have about $150,000 - $180,000 to potentially spend on a new microscope/digital vision system, and we are trying to determine which type of system would give us more relevant information about our product's components. We want to examine the following materials: the tips of ball point, fountain and roller ball pens (iron, tungsten carbide, brass, nickel-coated brass, nickel silver alloys, ceramic), plastic tip holders and ink reservoirs (polyethylene/polypropylene, nylon, PET), absorbent materials of various sizes and porosities (cellulosic, natural or artificial fibers). We are also interested in examining high-concentration micronized pigment dispersions to determine particle size (approximately 1 micron), shape and/or particle size distribution.
We are trying to decide between a tabletop SEM (either the Hitachi TM-1000 or the FEI Phenom) or a scanning laser microscope (Keyence VK-9700). We have a very limited amount of space to work with - anything larger than a desktop unit would require part of the budget to be spent on building a new room - and that is virtually impossible to get approved. Practically speaking, it's a desktop unit or nothing.
So, my questions are: Is there any way, short of sending many samples off to be examined, and then evaluating the results, to eliminate one or more of these units? Is there one that stands out for versatility or ease of use? How big a task is sample prep for these units? Additionally, have we missed a system to consider?
Please note that we have never looked at any of these components at higher than optical magnification before, so I can't tell you what features I need to see, because I have no idea what, if anything, that there is to see, or even whether or not the nanoscale detail makes a difference in the performance of our products. We will not be able to dedicate a person to the operation and maintenance of the system, so anything that takes much more than an hour or so of maintenance a week is going to be a big problem. I also need to point out that the closer the price gets to $100,000, the more competitive it is among all the instrumentation options we're considering, and the more likely it is to get approved.
Robert Zonis Product Development Chemist, LMTC Sanford L.P. - A Newell Rubbermaid Company 3 Sharpie Way Shelbyville, TN 37160 robert.zonis-at-sanford.com www.papermate.com
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==============================Original Headers============================== 10, 34 -- From Robert.Zonis-at-sanford.com Wed Jun 6 10:48:53 2007 10, 34 -- Received: from mail42.messagelabs.com (mail42.messagelabs.com [216.82.244.163]) 10, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l56FmpNJ030079 10, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 10:48:52 -0500 10, 34 -- X-VirusChecked: Checked 10, 34 -- X-Env-Sender: Robert.Zonis-at-sanford.com 10, 34 -- X-Msg-Ref: server-23.tower-42.messagelabs.com!1181144927!38267531!1 10, 34 -- X-StarScan-Version: 5.5.12.11; banners=sanford.com,-,- 10, 34 -- X-Originating-IP: [12.2.115.11] 10, 34 -- Received: (qmail 21873 invoked from network); 6 Jun 2007 15:48:47 -0000 10, 34 -- Received: from gnat.newellco.com (HELO nafepncsvpout2.nr.ad.newellco.com) (12.2.115.11) 10, 34 -- by server-23.tower-42.messagelabs.com with SMTP; 6 Jun 2007 15:48:47 -0000 10, 34 -- Received: from nafepncsefe02.nr.ad.newellco.com (unverified) by nafepncsvpout2.nr.ad.newellco.com 10, 34 -- (Content Technologies SMTPRS 4.3.17) with ESMTP id {T800d0f66ca0a059a211440-at-nafepncsvpout2.nr.ad.newellco.com} for {Microscopy-at-microscopy.com} ; 10, 34 -- Wed, 6 Jun 2007 10:48:47 -0500 10, 34 -- Received: from nashbsasebe01.nr.ad.newellco.com ([10.217.223.198]) by nafepncsefe02.nr.ad.newellco.com with Microsoft SMTPSVC(6.0.3790.1830); 10, 34 -- Wed, 6 Jun 2007 10:48:46 -0500 10, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 34 -- Content-class: urn:content-classes:message 10, 34 -- MIME-Version: 1.0 10, 34 -- Content-Type: text/plain; 10, 34 -- charset="iso-8859-1" 10, 34 -- Subject: New microscope purchase advice 10, 34 -- Date: Wed, 6 Jun 2007 10:48:45 -0500 10, 34 -- Message-ID: {8809C39726DC5F43824A3D3BE5D0286A0CAF0171-at-nashbsasebe01.nr.ad.newellco.com} 10, 34 -- X-MS-Has-Attach: 10, 34 -- X-MS-TNEF-Correlator: 10, 34 -- Thread-Topic: New microscope purchase advice 10, 34 -- Thread-Index: AceoUip05xeNL5+4QlCCM1LmkcEF1A== 10, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-sanford.com} 10, 34 -- To: {Microscopy-at-microscopy.com} 10, 34 -- X-OriginalArrivalTime: 06 Jun 2007 15:48:46.0866 (UTC) FILETIME=[2B2F6720:01C7A852] 10, 34 -- Content-Transfer-Encoding: 8bit 10, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l56FmpNJ030079 ==============================End of - Headers==============================
I would encourage you to investigate NT-MDT's Tomo, a unique combination of AFM and ultramicrotome, fitted with Media Cybernetics 3D constructor. It will provide the 3D information you need for accurate particle sizing/shape characterization as well as distribution calculations. Depending on how they are mounted, you can also use the AFM to characterize the surfaces you described, without having to coat or pump down a vacuum. Their new dealer in the US is Abeam, in Castro Valley, CA.
Also, depending on the size of the surface features, a good reflected light optical microscope, preferably fitted DIC or Hoffman Modulation Contrast, and Polarizers, would go a long way toward routine evaluations. MME provides specialized courses in both these areas. If you are interested, please contact me off-line.
CAVEAT: MME no longer has any financial relationship with NT-MDT but does make its living through customized, on-site courses.
Hope this was helpful, Barbara Foster, President
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
MME is now scheduling customized, on-site courses through September. Call us today for details.
P. S. Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 10:57 AM 6/6/2007, Robert.Zonis-at-sanford.com wrote:
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==============================Original Headers============================== 16, 17 -- From bfoster-at-mme1.com Wed Jun 6 12:23:44 2007 16, 17 -- Received: from mail.plhosting.com (qmail1e.plhosting.com [65.39.254.7]) 16, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56HNhHM016224 16, 17 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 12:23:44 -0500 16, 17 -- Message-Id: {200706061723.l56HNhHM016224-at-ns.microscopy.com} 16, 17 -- Received: (qmail 5612 invoked by uid 0); 6 Jun 2007 17:23:43 -0000 16, 17 -- Received: from unknown (HELO barbsd505.mme1.com) (bfoster-at-mme1.com-at-65.17.57.99) 16, 17 -- by qmail1e.plhosting.com with ESMTPA; 6 Jun 2007 17:23:42 -0000 16, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 16, 17 -- Date: Wed, 06 Jun 2007 12:23:06 -0500 16, 17 -- To: Robert.Zonis-at-sanford.com, microscopy-at-microscopy.com 16, 17 -- From: Barbara Foster {bfoster-at-mme1.com} 16, 17 -- Subject: Re: [Microscopy] New microscope purchase advice 16, 17 -- In-Reply-To: {200706061551.l56Fpi9p001428-at-ns.microscopy.com} 16, 17 -- References: {200706061551.l56Fpi9p001428-at-ns.microscopy.com} 16, 17 -- Mime-Version: 1.0 16, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Every once in a while we get a request to image particles which have been ingested by organisms, but this latest one seems especially tricky.
We are being asked to image carbon nanomaterials, such as nanowires, and how they distribute themselves in the tissues and organs of living organisms. My first search of the literature (ongoing, by the way) indicates that I'm not the only one having trouble with this. Aside from the obvious problems of differentiating 3-D structures in nanometers-thick sections, there is the problem of seeing low-contrast carbon in carbon-rich tissues.
My first thoughts were that adding electron density to the carbon nanothingys would make them easier to see, both in thin sections and in fractured specimens for SEM. At least this could help localize them, even if seeing their true shapes remains a problem. How to do this is another thing. Probably the particles would have to be augmented with metals before being released into the organisms' environment, and that would be a task for the makers and users of the nanoparticles. And would that affect their final distribution in the creatures? From our end, I'm trying to think of how to adapt immunolabeling techniques to this.
Has anybody run across this problem and have thoughts they'd be willing to share? The PI on this one has high hopes that we can put the nano-rabbit of this tiny, little hat.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 8, 23 -- From TindallR-at-missouri.edu Wed Jun 6 15:14:44 2007 8, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56KEhpv004593 8, 23 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 15:14:44 -0500 8, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 23 -- Wed, 6 Jun 2007 15:14:43 -0500 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="us-ascii" 8, 23 -- Subject: Carbon nanos in tissue: SEM/TEM 8, 23 -- Date: Wed, 6 Jun 2007 15:14:43 -0500 8, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BAC6-at-UM-XMAIL08.um.umsystem.edu} 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- Thread-Topic: Carbon nanos in tissue: SEM/TEM 8, 23 -- Thread-Index: Aceod1H9AEeN4khFSeSkBwoNR5LQVQ== 8, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 8, 23 -- To: {microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 06 Jun 2007 20:14:43.0146 (UTC) FILETIME=[51DE82A0:01C7A877] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l56KEhpv004593 ==============================End of - Headers==============================
As mentioned earlier, I have a movie made by Dr.deWith of the Dutch Polymer Institute (TU/E) showing the distribution of carbon nanotubes in epoxy. Because the AFM uses local differences in elasticity, there is none of the CNT in C-based tissue problem described below. Again, although I am no longer involved in this project, I am happy to forward a copy of the film to anyone who is interested. (It will come via www.YouSendIt.com, because of the large file size).
Dr. Anton Efimov of NT-MDT is the developer of this technique and is usually willing to run samples, schedule permitting. See his email in the CC: above.
NTA is no longer NT-MDT's agent here in the US. If you are interested in following this further, I recommend that you contact Dr. Yuri Bobrov, who is now with ABeam (Dealer principle, Dr. Sergey Babin - see email above).
As always, if there are further questions, please don't hesitate to call/email.
(Note: MME is no longer involved with this vendor).
Hope this was helpful, Barbara Foster, President
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
MME is now scheduling customized, on-site courses through September. Call us today for details.
P. S. Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 03:27 PM 6/6/2007, TindallR-at-missouri.edu wrote:
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==============================Original Headers============================== 20, 17 -- From bfoster-at-mme1.com Wed Jun 6 15:34:13 2007 20, 17 -- Received: from mail.plhosting.com (qmail1e.plhosting.com [65.39.254.7]) 20, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56KYDJZ016549 20, 17 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 15:34:13 -0500 20, 17 -- Message-Id: {200706062034.l56KYDJZ016549-at-ns.microscopy.com} 20, 17 -- Received: (qmail 15717 invoked by uid 0); 6 Jun 2007 20:34:12 -0000 20, 17 -- Received: from unknown (HELO barbsd505.mme1.com) (bfoster-at-mme1.com-at-65.17.57.99) 20, 17 -- by qmail1e.plhosting.com with ESMTPA; 6 Jun 2007 20:34:12 -0000 20, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 20, 17 -- Date: Wed, 06 Jun 2007 15:33:27 -0500 20, 17 -- To: TindallR-at-missouri.edu, microscopy-at-microscopy.com 20, 17 -- From: Barbara Foster {bfoster-at-mme1.com} 20, 17 -- Subject: Re: [Microscopy] Carbon nanos in tissue: SEM/TEM 20, 17 -- In-Reply-To: {200706062016.l56KGvAo008316-at-ns.microscopy.com} 20, 17 -- References: {200706062016.l56KGvAo008316-at-ns.microscopy.com} 20, 17 -- Mime-Version: 1.0 20, 17 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
I'm VERY interested. We need a database of dedicated people who are doing high school SEM, so that experience, problems, curricula, etc. can be shared. Will either of you be at M&M in Ft. Lauderdale? Please come to the MICRO booth so that we can try to organize something. Or send me an Email. If anyone else who is doing HS SEM, including those associated with the FEI & RJ Lee school SEM programs, is reading this, we need to talk to you too!
What is my motive, other than trying to get you together? Project MICRO is MSA's outreach program for middle schools (see URL below). I'd like to convince all of you to encourage introductory LM in your "feeder" middle schools; SEM is an abrupt way to begin.
Caroline
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==============================Original Headers============================== 4, 19 -- From schooley-at-mcn.org Wed Jun 6 16:16:27 2007 4, 19 -- Received: from dns3.mcn.org (dns3.mcn.org [216.150.240.32]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56LGQ0V028666 4, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 6 Jun 2007 16:16:27 -0500 4, 19 -- Received: from [66.52.170.6] (helo=[10.0.1.2]) 4, 19 -- by dns3.mcn.org with esmtpa (Exim 4.60) 4, 19 -- (envelope-from {schooley-at-mcn.org} ) 4, 19 -- id JJ8GF6-000LT6-59; Wed, 06 Jun 2007 14:16:22 -0700 4, 19 -- Mime-Version: 1.0 4, 19 -- Message-Id: {a06200701c28ccf4911d0-at-[10.0.1.2]} 4, 19 -- In-Reply-To: {200706061316.l56DGlWV025688-at-ns.microscopy.com} 4, 19 -- References: {200706061316.l56DGlWV025688-at-ns.microscopy.com} 4, 19 -- Date: Wed, 6 Jun 2007 14:18:01 -0700 4, 19 -- To: dljones-at-bestweb.net, donovan.leonard-at-gmail.com 4, 19 -- From: Caroline Schooley {schooley-at-mcn.org} 4, 19 -- Subject: [Microscopy] Re: Table Top SEM & High School Nano Course 4, 19 -- Cc: Microscopy-at-MSA.Microscopy.Com, john_mackenzie-at-ncsu.edu, 4, 19 -- tpepper-at-iastate.edu, murphyjudy-at-comcast.net 4, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Has the M&M 2007 daily schedule of papers and symposia been published yet? I didn't find it on the meeting web site?
thanks, Henk
Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 6, 23 -- From colijn.1-at-osu.edu Wed Jun 6 16:18:26 2007 6, 23 -- Received: from ER6S1.ENG.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56LIPD0032266 6, 23 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 16:18:26 -0500 6, 23 -- Received: from CONVERSION-DAEMON.er6s1.eng.ohio-state.edu by 6, 23 -- er6s1.eng.ohio-state.edu (PMDF V6.2-1x11 #31056) 6, 23 -- id {01MHH5RIFKUOAA9XBW-at-er6s1.eng.ohio-state.edu} for 6, 23 -- microscopy-at-microscopy.com; Wed, 06 Jun 2007 17:18:24 -0400 (EDT) 6, 23 -- Received: from HOC1.ecr6.ohio-state.edu 6, 23 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.eng.ohio-state.edu 6, 23 -- (PMDF V6.2-1x11 #31056) 6, 23 -- with ESMTPA id {01MHH5RI2SAUA9PSMS-at-er6s1.eng.ohio-state.edu} for 6, 23 -- microscopy-at-microscopy.com; Wed, 06 Jun 2007 17:18:24 -0400 (EDT) 6, 23 -- Date: Wed, 06 Jun 2007 17:19:11 -0400 6, 23 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 6, 23 -- Subject: M&M 2007 detailed program 6, 23 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 6, 23 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 6, 23 -- Message-id: {7.0.1.0.2.20070606171402.036b0d80-at-osu.edu} 6, 23 -- MIME-version: 1.0 6, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 6, 23 -- Content-type: text/plain; charset=us-ascii; format=flowed 6, 23 -- X-Env-From: auth/colijn.1-at-osu.edu ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jenniferwal-at-cs.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, June 6, 2007 at 13:16:12 ---------------------------------------------------------------------------
Email: jenniferwal-at-cs.com Name: Jennifer Wallace
Organization: Academic Coaching
Education: 9-12th Grade High School
Location: Evanston, IL USA
Question: I am working with a junior in high school who is very interested in this field. We are trying to find an internship or volunteer experience for her and need help! She is in the most rigorous science program at her high school and is an excellent photographer, which is why she is interested in this field in college and beyond.
I know that vendors/vendor employees are active and involved in the MSA and contribute greatly to the dialogs here. However, I personally feel that this message reply is a bit over the top in commercializing the List. Every question to the List can't be answered "you should try AFM" and go on to plug the company and it's products. The response seems only slightly related to the essence of the original question. How is AFM going to visualize carbon nanotubes inside a cell that is inside a tissue? This was a repeat of the response to recent technical questions on vacuum metal shadowing technical problems. It is fine to bring attention to alternate methods, but primarily we should be good listeners and try to help people with the problem they have asked about. Metal shadowing is a proven technique - with limitations, same as ALL techniques - that people use routinely; one can scan relatively large areas at high resolution for the best area. It is a method suitable to the purpose. The DNA is already bulked up artificially by the preparation method and the metal shadowing adds a known factor to that size. But {visualizing} the DNA/plasmid is often the key, not the exact molecular size. One vendor sent a link to an image of a 1um square scan of DNA; the image was quite blurry, 2 nm/pixel resolution. I'm sure this is a "display" result - the best they have. Finding a plasmid on a sheet of carbon/mica for AFM scanning can't be as routine as the "traditional" method of viewing the shadowed material in a TEM. It is a bit of comparing apples and oranges.
So AFM is not the one answer to all questions, and please go light on the commercial plugs. It seems that vendors were more respectful of this "line" in the past.
Thanks for allowing my rant,
Dale Callaham
bfoster-at-mme1.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Randy, } } This is another one of those "TOMO" applications. } } As mentioned earlier, I have a movie made by Dr.deWith of the Dutch Polymer Institute (TU/E) showing the distribution of carbon nanotubes in epoxy. Because the AFM uses local differences in elasticity, there is none of the CNT in C-based tissue problem described below. Again, although I am no longer involved in this project, I am happy to forward a copy of the film to anyone who is interested. (It will come via www.YouSendIt.com, because of the large file size). } } Dr. Anton Efimov of NT-MDT is the developer of this technique and is usually willing to run samples, schedule permitting. See his email in the CC: above. } } NTA is no longer NT-MDT's agent here in the US. If you are interested in following this further, I recommend that you contact Dr. Yuri Bobrov, who is now with ABeam (Dealer principle, Dr. Sergey Babin - see email above). } } As always, if there are further questions, please don't hesitate to call/email. } } (Note: MME is no longer involved with this vendor). } } Hope this was helpful, } Barbara Foster, President } } Microscopy/Microscopy Education } 313 S Jupiter Rd, Suite 100 } Allen, TX 75002 } P: 972-954-8011 } W: www.MicroscopyEducation.com } } } MME is now scheduling customized, on-site courses through September. Call us today for details. } } P. S. } Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011. } } } } } At 03:27 PM 6/6/2007, TindallR-at-missouri.edu wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear Listers, } } } } Every once in a while we get a request to image particles which have } } been ingested by organisms, but this latest one seems especially tricky. } } } } We are being asked to image carbon nanomaterials, such as nanowires, and } } how they distribute themselves in the tissues and organs of living } } organisms. My first search of the literature (ongoing, by the way) } } indicates that I'm not the only one having trouble with this. Aside } } from the obvious problems of differentiating 3-D structures in } } nanometers-thick sections, there is the problem of seeing low-contrast } } carbon in carbon-rich tissues. } } } } My first thoughts were that adding electron density to the carbon } } nanothingys would make them easier to see, both in thin sections and in } } fractured specimens for SEM. At least this could help localize them, } } even if seeing their true shapes remains a problem. How to do this is } } another thing. Probably the particles would have to be augmented with } } metals before being released into the organisms' environment, and that } } would be a task for the makers and users of the nanoparticles. And } } would that affect their final distribution in the creatures? From our } } end, I'm trying to think of how to adapt immunolabeling techniques to } } this. } } } } Has anybody run across this problem and have thoughts they'd be willing } } to share? The PI on this one has high hopes that we can put the } } nano-rabbit of this tiny, little hat. } } } } Cheers, } } Randy } } } } Randy Tindall } } Senior EM Specialist } } Electron Microscopy Core Facility---We Do Small Well! } } W125 Veterinary Medicine } } University of Missouri } } Columbia, MO 65211 } } Tel: (573) 882-8304 } } Fax: (573) 884-2227 } } Email: tindallr-at-missouri.edu } } Web: http://www.emc.missouri.edu } } On-line calendar: } } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } } Week&NavType=Both&Type=TimePlan } } } } } } ==============================Original Headers============================== } } 8, 23 -- From TindallR-at-missouri.edu Wed Jun 6 15:14:44 2007 } } 8, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) } } 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56KEhpv004593 } } 8, 23 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 15:14:44 -0500 } } 8, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } } 8, 23 -- Wed, 6 Jun 2007 15:14:43 -0500 } } 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } } 8, 23 -- Content-class: urn:content-classes:message } } 8, 23 -- MIME-Version: 1.0 } } 8, 23 -- Content-Type: text/plain; } } 8, 23 -- charset="us-ascii" } } 8, 23 -- Subject: Carbon nanos in tissue: SEM/TEM } } 8, 23 -- Date: Wed, 6 Jun 2007 15:14:43 -0500 } } 8, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BAC6-at-UM-XMAIL08.um.umsystem.edu} } } 8, 23 -- X-MS-Has-Attach: } } 8, 23 -- X-MS-TNEF-Correlator: } } 8, 23 -- Thread-Topic: Carbon nanos in tissue: SEM/TEM } } 8, 23 -- Thread-Index: Aceod1H9AEeN4khFSeSkBwoNR5LQVQ== } } 8, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } } 8, 23 -- To: {microscopy-at-microscopy.com} } } 8, 23 -- X-OriginalArrivalTime: 06 Jun 2007 20:14:43.0146 (UTC) FILETIME=[51DE82A0:01C7A877] } } 8, 23 -- Content-Transfer-Encoding: 8bit } } 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l56KEhpv004593 } } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 20, 17 -- From bfoster-at-mme1.com Wed Jun 6 15:34:13 2007 } 20, 17 -- Received: from mail.plhosting.com (qmail1e.plhosting.com [65.39.254.7]) } 20, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56KYDJZ016549 } 20, 17 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 15:34:13 -0500 } 20, 17 -- Message-Id: {200706062034.l56KYDJZ016549-at-ns.microscopy.com} } 20, 17 -- Received: (qmail 15717 invoked by uid 0); 6 Jun 2007 20:34:12 -0000 } 20, 17 -- Received: from unknown (HELO barbsd505.mme1.com) (bfoster-at-mme1.com-at-65.17.57.99) } 20, 17 -- by qmail1e.plhosting.com with ESMTPA; 6 Jun 2007 20:34:12 -0000 } 20, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 20, 17 -- Date: Wed, 06 Jun 2007 15:33:27 -0500 } 20, 17 -- To: TindallR-at-missouri.edu, microscopy-at-microscopy.com } 20, 17 -- From: Barbara Foster {bfoster-at-mme1.com} } 20, 17 -- Subject: Re: [Microscopy] Carbon nanos in tissue: SEM/TEM } 20, 17 -- In-Reply-To: {200706062016.l56KGvAo008316-at-ns.microscopy.com} } 20, 17 -- References: {200706062016.l56KGvAo008316-at-ns.microscopy.com} } 20, 17 -- Mime-Version: 1.0 } 20, 17 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 21 -- From dac-at-research.umass.edu Thu Jun 7 10:40:53 2007 8, 21 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57FerNb024904 8, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 10:40:53 -0500 8, 21 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 8, 21 -- (authenticated bits=0) 8, 21 -- by race4.oit.umass.edu (8.13.7/8.13.7) with ESMTP id l57FeqlA029015 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 11:40:52 -0400 8, 21 -- Message-ID: {4668359D.3010902-at-research.umass.edu} 8, 21 -- Date: Thu, 07 Jun 2007 11:43:09 -0500 8, 21 -- From: Dale Callaham {dac-at-research.umass.edu} 8, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.4) Gecko/20070509 SeaMonkey/1.1.2 8, 21 -- MIME-Version: 1.0 8, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 8, 21 -- Subject: Re: [Microscopy] Re: Carbon nanos in tissue: SEM/TEM 8, 21 -- References: {200706062039.l56Kd7jT026138-at-ns.microscopy.com} 8, 21 -- In-Reply-To: {200706062039.l56Kd7jT026138-at-ns.microscopy.com} 8, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 21 -- Content-Transfer-Encoding: 7bit 8, 21 -- X-Whitelist: TRUE ==============================End of - Headers==============================
MSA 2007 is just a short 8 weeks away in beautiful Ft. Lauderdale, Florida !!!!
The reduced rate registration deadline is July 6, so please register early.
This is the second announcement for students who will attend the Microscopy and Microanalysis Meeting in Ft. Lauderdale, Fl, August 5-9, 2007 and would like to apply for a Student Bursary to help defray meeting costs. The complete description of the Bursary is listed below, including the registration process.
In addition, technicians, post-docs, faculty and presenters may also apply for a bursary in exchange for working to support the meeting.
The contact persons this year who will coordinate the student volunteers are;
Bill Monroe monroe-at-emcenter.msstate.edu Amanda Lawrence alawrence-at-emcenter.msstate.edu Mike Miller millem1-at-auburn.edu
STUDENT BURSARIES
The Microscopy Society of America values student members and recognizes that they are the future of both the society and the field of microscopy in general. MSA is therefore pleased to offer student bur-saries of $200 to registered students. The most important purpose of these bursaries is to encourage students to attend the annual MSA/MAS Microscopy and Microanalysis meeting, where these young sci-entists can meet and interact with the established microscopy community as well as assisting with the meeting.
Each bursary recipient will be expected to work for a minimum of 20 hours during the meeting and/or at the pre-meeting weekend events. A student may work up to an additional 20 hours, for a total of 40 hours. These extra hours will add to the bursary total at a rate of $10 per hour. The maximum bursary will therefore be $400. The duties will involve, but are not necessarily limited to, providing support in symposia (helping with audio-visual needs, maintaining an attendance count, and helping speakers set up for their presentation), staffing the MSA Megabooth, and monitoring use of the Internet Cafe. Applicants for the bursaries must be members of MSA or MAS, and enrolled as students at a recognized educational institution. All MSA or MAS student members are eligible for bursaries, including those who are recipients of MSA Presidential Scholars Awards and MAS Distinguished Scholar Awards. Eligible students may apply for bursaries when registering for the conference on the conference website, or at on-site registration. Bursaries are limited and early application is encouraged. How it works:
The registration form for the meeting will have a check box indicating that the applicant is a registered student and is requesting a bursary. The check box will have a note beside it reminding the applicant that the bursary requires them to work at the meeting. Students who have applied for bursaries will receive letters from MSA explaining the conditions that they need to satisfy in order to receive the bursaries. The tasks at the meeting will be allocated by the Student Worker Organizers Sub-Committee of the Education Committee. When students pick up their registration materials at the meeting, they will receive assignment forms indicating the specific tasks they are to perform, and the person(s) they need to contact in order to carry out those tasks. Each stu-dent's assignment forms must be signed by all of the contact persons listed, indicating that all assigned tasks have been performed. Upon completion of assignments and submission of the signed forms, the bursary check will be issued by the appropriate representative at the registration desk.
Should you have questions concerning the process, please contact:
Bill Monroe monroe-at-emcenter.msstate.edu
-- Bill Monroe Electron Microscope Center 103 Clay Lyle Entomology Building Mississippi State University Mississippi State, MS 39762 (662)-325-3019 Work (662)-323-5246 Home (662)-325-0246 Fax
==============================Original Headers============================== 17, 15 -- From monroe-at-emcenter.msstate.edu Thu Jun 7 10:52:13 2007 17, 15 -- Received: from Ra.MsState.Edu (Ra.msstate.edu [130.18.80.10]) 17, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57FqC7o004127 17, 15 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 10:52:12 -0500 17, 15 -- Received: from [130.18.130.108] (ws108-130.clay-lyle.dynamic.msstate.edu [130.18.130.108]); 17, 15 -- by Ra.MsState.Edu (8.13.8/8.12.8/ra_1.2) with SMTP; 17, 15 -- id l57FqBOG005896 for {"Microscopy-at-microscopy.com"} ; Thu, 7 Jun 2007 10:52:12 -0500 (CDT) 17, 15 -- Mime-Version: 1.0 17, 15 -- Message-Id: {a06230934c28dd7b91c59-at-[130.18.130.108]} 17, 15 -- Date: Thu, 7 Jun 2007 10:52:11 -0500 17, 15 -- To: "Microscopy-at-microscopy.com"-at-Ra.MsState.Edu 17, 15 -- From: "William A. Monroe" {monroe-at-emcenter.msstate.edu} 17, 15 -- Subject: MSA 2007 Meeting: Request for Student and Other Volunteers 17, 15 -- (Bursaries Available) 17, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
To clarify: I am a consultant who has exposure to new technology and, if you review my messages over the years, you will find a large number of different technologies and vendors mentioned.
I did have a chance to work with NT-MDT (no longer)... and found that AFM, like other technologies (ex: interferometry) was under-utilized.
Regarding giving a plug to one vendor: TOMO is unique. There is no one else has integrated an AFM with an ultramicrotome for serial sections... so there IS no one else to mention.
Hope this is helpful.
Barbara
At 10:44 AM 6/7/2007, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 14, 17 -- From bfoster-at-mme1.com Thu Jun 7 11:10:59 2007 14, 17 -- Received: from mail.plhosting.com (qmail1h.plhosting.com [65.39.254.134]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57GAwf8015890 14, 17 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 11:10:59 -0500 14, 17 -- Message-Id: {200706071610.l57GAwf8015890-at-ns.microscopy.com} 14, 17 -- Received: (qmail 1085 invoked by uid 0); 7 Jun 2007 16:10:57 -0000 14, 17 -- Received: from unknown (HELO barbsd505.mme1.com) (bfoster-at-mme1.com-at-65.17.57.99) 14, 17 -- by qmail1h.plhosting.com with ESMTPA; 7 Jun 2007 16:10:56 -0000 14, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 14, 17 -- Date: Thu, 07 Jun 2007 11:10:22 -0500 14, 17 -- To: dac-at-research.umass.edu, microscopy-at-microscopy.com 14, 17 -- From: Barbara Foster {bfoster-at-mme1.com} 14, 17 -- Subject: Re: [Microscopy] Carbon nanos in tissue: SEM/TEM 14, 17 -- In-Reply-To: {200706071544.l57Fi3p9028702-at-ns.microscopy.com} 14, 17 -- References: {200706071544.l57Fi3p9028702-at-ns.microscopy.com} 14, 17 -- Mime-Version: 1.0 14, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both oddioeng-at-aol.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: oddioeng-at-aol.com Name: J. Allen Williams, Jr.
Organization: ORDELA, Inc.
Title-Subject: [Filtered] UPDATE of UC-4 Dewar Re-evacuation port
Question: Hello, I have discovered the answer to my question from yesterday, the ball in the evacuation port is the vacuum valve for the Dewar. If the Dewar is near atmosphere and if one needs to re-evacuate, you may use either a specified valve or what I did - (which is somewhat unorthodox) - connect your evaluation pump to the port and the ball 'magically levitates' from the o-ring seal. (This is also true of smaller LINDE Dewars). Therefore, the main seal of this type Dewar re-evacuation port seals the vacuum inside of the vacuum insulator by the atmospheric pressure on the ball and the o-ring seat.
I found this out by connecting my old Welch 1402 vacuum pump to the Dewar port and heard a click, and the click was a sound of the ball being sucked towards the vacuum pump. Mystery solvÈd. Thought I would let everyone know. It is amazing that atmospheric pressure can seal the vacuum in a liquid nitrogen/argon Dewar. Thanks for all the suggestions and hope this may help for reference in the future.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both K.venner-at-ion.ucl.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ecd10-at-psu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ecd10-at-psu.edu Name: Elizabeth Dickey
Organization: Pennsylvania State University
Title-Subject: [Filtered] Postdoctoral Position Open
Question: POST-DOCTORAL POSITION in Transmission Electron Microscopy at The Pennsylvania State University
A postdoctoral position is available in the area of transmission electron microscopy of amorphous dielectrics beginning July 1, 2007. The research project focuses on understanding structure and chemistry of amorphous metal oxides for capacitor applications. Through a variety of electron imaging, spectroscopy and diffraction techniques, including fluctuation electron microscopy, we aim to quantify structure and chemistry of amorphous metal-oxide layers to help establish processing/structure/property relationships for this class of materials. Most of the research will be conducted on a JEOL 2010F Field Emission TEM/STEM outfitted with an EDAX energy dispersive x-ray spectrometer (EDS), Gatan Enfina electron energy loss spectrometer (EELS), high-angle annular dark field STEM detector, and acquisition hardware and software for spectrum imaging. The ideal candidate for this position will have experience in HREM, STEM, EDS and EELS. The salary will be commensurate with qualifications and experience.
Please forward questions or send applications to:
Professor Elizabeth Dickey Department of Materials Science and Engineering The Pennsylvania State University 223 Materials Research Building University Park, PA 16802 USA
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both skooi-at-mit.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: skooi-at-mit.edu Name: Steven Kooi
Organization: ISN -at- MIT
Title-Subject: [Filtered] Research Specialist Position
Question: The Institute for Soldier Nanotechnologies (ISN) at MIT is looking for a research specialist to handle multiple responsibilities including transmission electron microscopy (TEM), scanning electron microscopy (SEM), focused ion beam (FIB), and atomic force microscopy (AFM). Primary responsibilities include providing introductory and more advanced training of users on electron microscopes, and assisting ISN researchers with sample preparation and characterization using electron and surface microscopy techniques. Other responsibilities may include daily operation, maintenance, and upkeep of several pieces of advanced nano-characterization instrumentation and related sample preparation tools; and serving as the primary contact with the equipment manufacturer's service personnel to quickly resolve any serious instrumentation issues.
The position announcement can be found at http://sh.webhire.com/servlet/av/jd?ai=631&ji=2025576&sn=I
Additional information on ISN is available at http://web.mit.edu/isn.
I agree that keeping the list as "commercial free" as possible is a good thing. However, I didn't really interpret Barbara's response to my question as a commercial plug as much as pointing out a potentially useful technology. In fact, I believe she pointed out that MME is no longer affiliated with that vendor. Personally, I'll take any advice that helps our lab help our clients with their research, whether from consultants, vendors, or fellow techs.
I have had many vendors contact me off-list with suggestions involving their products, when they thought their responses might be seen as too commercial. To me that's a very courteous and professional response. That said, I hope that those who are not formally affiliated with a vendor won't hesitate to point out useful techniques or gizmos to the list at large.
My personal opinion is that this particular response was not out of line.
It's good to bring these issues up occasionally, so this is not a dig at Dale, by any means.
Cheers, Randy
-----Original Message----- X-from: dac-at-research.umass.edu [mailto:dac-at-research.umass.edu] Sent: Thursday, June 07, 2007 10:42 AM To: Tindall, Randy D.
Dear Microscopists,
I know that vendors/vendor employees are active and involved in the MSA and contribute greatly to the dialogs here. However, I personally feel that this message reply is a bit over the top in commercializing the List. Every question to the List can't be answered "you should try AFM" and go on to plug the company and it's products. The response seems only slightly related to the essence of the original question. How is AFM going to visualize carbon nanotubes inside a cell that is inside a tissue? This was a repeat of the response to recent technical questions on vacuum metal shadowing technical problems. It is fine to bring attention to alternate methods, but primarily we should be good listeners and try to help people with the problem they have asked about.
Metal shadowing is a proven technique - with limitations, same as ALL techniques - that people use routinely; one can scan relatively large areas at high resolution for the best area. It is a method suitable to the purpose. The DNA is already bulked up artificially by the preparation method and the metal shadowing adds a known factor to that size. But {visualizing} the DNA/plasmid is often the key, not the exact molecular size. One vendor sent a link to an image of a 1um square scan of DNA; the image was quite blurry, 2 nm/pixel resolution. I'm sure this is a "display" result - the best they have. Finding a plasmid on a sheet of carbon/mica for AFM scanning can't be as routine as the "traditional"
method of viewing the shadowed material in a TEM. It is a bit of comparing apples and oranges.
So AFM is not the one answer to all questions, and please go light on the commercial plugs. It seems that vendors were more respectful of this "line" in the past.
Thanks for allowing my rant,
Dale Callaham
bfoster-at-mme1.com wrote: } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Randy, } } This is another one of those "TOMO" applications. } } As mentioned earlier, I have a movie made by Dr.deWith of the Dutch Polymer Institute (TU/E) showing the distribution of carbon nanotubes in epoxy. Because the AFM uses local differences in elasticity, there is none of the CNT in C-based tissue problem described below. Again, although I am no longer involved in this project, I am happy to forward a copy of the film to anyone who is interested. (It will come via www.YouSendIt.com, because of the large file size). } } Dr. Anton Efimov of NT-MDT is the developer of this technique and is usually willing to run samples, schedule permitting. See his email in the CC: above. } } NTA is no longer NT-MDT's agent here in the US. If you are interested in following this further, I recommend that you contact Dr. Yuri Bobrov, who is now with ABeam (Dealer principle, Dr. Sergey Babin - see email above). } } As always, if there are further questions, please don't hesitate to call/email. } } (Note: MME is no longer involved with this vendor). } } Hope this was helpful, } Barbara Foster, President } } Microscopy/Microscopy Education } 313 S Jupiter Rd, Suite 100 } Allen, TX 75002 } P: 972-954-8011 } W: www.MicroscopyEducation.com } } } MME is now scheduling customized, on-site courses through September. Call us today for details. } } P. S. } Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011. } } } } } At 03:27 PM 6/6/2007, TindallR-at-missouri.edu wrote: } } } } } --------------------------------------------------------------------- } } ------- The Microscopy ListServer -- CoSponsor: The Microscopy } } Society of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------- } } ------- } } } } Dear Listers, } } } } Every once in a while we get a request to image particles which have } } been ingested by organisms, but this latest one seems especially tricky. } } } } We are being asked to image carbon nanomaterials, such as nanowires, } } and how they distribute themselves in the tissues and organs of } } living organisms. My first search of the literature (ongoing, by the
} } way) indicates that I'm not the only one having trouble with this. } } Aside from the obvious problems of differentiating 3-D structures in } } nanometers-thick sections, there is the problem of seeing low-contrast
} } carbon in carbon-rich tissues. } } } } My first thoughts were that adding electron density to the carbon } } nanothingys would make them easier to see, both in thin sections and } } in fractured specimens for SEM. At least this could help localize } } them, even if seeing their true shapes remains a problem. How to do } } this is another thing. Probably the particles would have to be } } augmented with metals before being released into the organisms' } } environment, and that would be a task for the makers and users of the
} } nanoparticles. And would that affect their final distribution in the
} } creatures? From our end, I'm trying to think of how to adapt } } immunolabeling techniques to this. } } } } Has anybody run across this problem and have thoughts they'd be } } willing to share? The PI on this one has high hopes that we can put } } the nano-rabbit of this tiny, little hat. } } } } Cheers, } } Randy } } } } Randy Tindall } } Senior EM Specialist } } Electron Microscopy Core Facility---We Do Small Well! } } W125 Veterinary Medicine } } University of Missouri } } Columbia, MO 65211 } } Tel: (573) 882-8304 } } Fax: (573) 884-2227 } } Email: tindallr-at-missouri.edu } } Web: http://www.emc.missouri.edu } } On-line calendar: } } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amou } } nt= } } Week&NavType=Both&Type=TimePlan } } } } } } ==============================Original } } Headers============================== } } 8, 23 -- From TindallR-at-missouri.edu Wed Jun 6 15:14:44 2007 8, 23 --
} } Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) } } 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56KEhpv004593 } } 8, 23 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jun 2007 15:14:44 -0500 } } 8, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } } 8, 23 -- Wed, 6 Jun 2007 15:14:43 -0500 } } 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- } } Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0
I'd like to convince all of you to encourage inductory imaging and measurement in your "feeder" middle schools. It has been my experience that too few students understand anything about shape and size. Those who are unable to measure the diameter and length of a broomstick are going to have a difficult time on a nano-rod.
just my two cents............
JQuinn
PS...........OoO away........
} From mail-at-ns.microscopy.com Wed Jun 6 17:14:41 2007 } Date: Wed, 6 Jun 2007 16:17:10 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: schooley-at-mcn.org } Reply-to: schooley-at-mcn.org } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] Re: Table Top SEM & High School Nano Course } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I'm VERY interested. We need a database of dedicated people who are } doing high school SEM, so that experience, problems, curricula, etc. } can be shared. Will either of you be at M&M in Ft. Lauderdale? } Please come to the MICRO booth so that we can try to organize } something. Or send me an Email. If anyone else who is doing HS SEM, } including those associated with the FEI & RJ Lee school SEM programs, } is reading this, we need to talk to you too! } } What is my motive, other than trying to get you together? Project } MICRO is MSA's outreach program for middle schools (see URL below). } I'd like to convince all of you to encourage introductory LM in your } "feeder" middle schools; SEM is an abrupt way to begin. } } Caroline } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Donovan, } } } } I have helped get a SEM put into my local high school. The science teacher } } there now is able to offer 8 college credits to students that take his } } advanced program related to this instrument. Mark would be able to talk to } } you more about that aspect and how he integrates it into his curriculum. I } } have cc'd him with this email so you'll have his email address. This is } } still in the beginning stages so there is a lot yet to know and learn. But } } I think it is an absolutely fabulous opportunity for learning... } } } } In the process of getting the SEM into my local high school, I have } } learned a fair amount about SEM usage in high schools in the US and a bit } } internationally. There are pockets of high schools around the USA that } } have this kind of technology operating in high schools, but not a lot. } } Germany has some high schools using it, apparently Japan has it } } extensively used throughout their high school system. } } } } You are using Au and Ag particles. My experience indicates high school } } students seem more interested in biological samples rather than materials. } } It would be very useful if a materials curriculum could be developed that } } would interest students at this level. } } } } Finding teachers and school systems that are open to this and have the } } science background to make it work well, I think is a large hurdle. If a } } solid curriculm could be put together that would work as a framework for } } teachers to use, I think that would be very helpful. } } } } Schools are, justifiably, reluctant to use this technology as maintaining } } these machines is quite expensive and most schools run on very tight } } budgets. In the cases where I have seen SEM's working in high schools, } } there has usually been someone that is knowledgable about the instruments } } and can maintain them for the school pro-bono. These individuals have been } } key in getting SEM's into high schools. (There may be a program in western } } PA that's an exception to this, but I've not had any luck contacting } } them.) } } } } Do send me your curriculum and further information about what you are } } doing. I can give you more details of programs I've discovered if you } } wish, but I don't think those details are of general interest to the list } } (do correct me if I'm wrong). } } } } dj } } } } On Tue, 5 Jun 2007, donovan.leonard-at-gmail.com wrote: } } } } } Hello Listsers - } } } } } } Some days ago there was a question posed about the educational benefits } } } of table top SEM and nano-scale samples. In a few weeks I'll post my } } } experiences using a Hitachi TM-1000 table top SEM in a Nanotechnology } } } course for high schoolers as part of the Duke University Talent } } } Identification Program (TIP). More about the program can be found at } } } 'www.tip.duke.edu'. I've chosen the TM-1000 to introduce the students } } } to the micro and nanoscale, and we will be using the SEM, in addition to } } } AFM and HRTEM to characterize Au and Ag nanoparticles the students } } } synthesize as part of a experiential learning activity. } } } } } } In my opinion the table top SEM will allow the students hands-on } } } experience with an electron microscope. Not that it will resolve atomic } } } columns in nanoparticles, but as a way to introduce them to the scale of } } } things. The intention is to let the students choose ANY samples they } } } are interested in and let them discover the detail of features on the } } } micro and nanoscale. } } } } } } If anyone has had experience integrating these microscopies into a high } } } school course on nanotechnology I would be most interested in learning } } } more about how it was accomplished and the outcomes. I would also be } } } more than happy to share the syllabus created for the course if requested. } } } } } } Stay tuned, I'll post the student's data and feedback on the table top } } } SEM, AFM and TEM. } } } } } Donovan } -- } Caroline Schooley } Project MICRO Coordinator } Microscopy Society of America } Box 117, 45301 Caspar Point Road } Caspar, CA 95420 } Phone/FAX (707)964-9460 } Project MICRO: http://www.microscopy.org/ProjectMICRO } } ==============================Original Headers============================== } 4, 19 -- From schooley-at-mcn.org Wed Jun 6 16:16:27 2007 } 4, 19 -- Received: from dns3.mcn.org (dns3.mcn.org [216.150.240.32]) } 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l56LGQ0V028666 } 4, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 6 Jun 2007 16:16:27 -0500 } 4, 19 -- Received: from [66.52.170.6] (helo=[10.0.1.2]) } 4, 19 -- by dns3.mcn.org with esmtpa (Exim 4.60) } 4, 19 -- (envelope-from {schooley-at-mcn.org} ) } 4, 19 -- id JJ8GF6-000LT6-59; Wed, 06 Jun 2007 14:16:22 -0700 } 4, 19 -- Mime-Version: 1.0 } 4, 19 -- Message-Id: {a06200701c28ccf4911d0-at-[10.0.1.2]} } 4, 19 -- In-Reply-To: {200706061316.l56DGlWV025688-at-ns.microscopy.com} } 4, 19 -- References: {200706061316.l56DGlWV025688-at-ns.microscopy.com} } 4, 19 -- Date: Wed, 6 Jun 2007 14:18:01 -0700 } 4, 19 -- To: dljones-at-bestweb.net, donovan.leonard-at-gmail.com } 4, 19 -- From: Caroline Schooley {schooley-at-mcn.org} } 4, 19 -- Subject: [Microscopy] Re: Table Top SEM & High School Nano Course } 4, 19 -- Cc: Microscopy-at-MSA.Microscopy.Com, john_mackenzie-at-ncsu.edu, } 4, 19 -- tpepper-at-iastate.edu, murphyjudy-at-comcast.net } 4, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
==============================Original Headers============================== 12, 12 -- From jquinn-at-www.matscieng.sunysb.edu Thu Jun 7 12:58:22 2007 12, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 12, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57HwMgC021518 12, 12 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 12:58:22 -0500 12, 12 -- Received: (from jquinn-at-localhost) 12, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id l57Hthw01791 12, 12 -- for microscopy-at-microscopy.com; Thu, 7 Jun 2007 13:55:43 -0400 12, 12 -- Date: Thu, 7 Jun 2007 13:55:43 -0400 12, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 12, 12 -- Message-Id: {200706071755.l57Hthw01791-at-www.matscieng.sunysb.edu} 12, 12 -- To: microscopy-at-microscopy.com 12, 12 -- Subject: Re: [Microscopy] Re: Table Top SEM & High School Nano Course ==============================End of - Headers==============================
Are there any really tiny cameras about, or at least, fibre-optic lenses/objectives? I ask because we have a couple of applications that could use one. We're interested in roots growing down pre-existing soil pores, and would like to watch what they're doing down there, either with a moving imaging device of some kind, or a series of portholes to watch roots growing past. Another group is screening different lines of cereals, in particular, looking at development of the floral meristem. This object of desire is enclosed in many leaves at ground level in a cereal plant, we'd like to poke a small fibre-optic in to watch its development over 3-6 days, rather than having to destroy many plants to follow the stages of development.
I've had a rummage through numerous websites, and sent off some email enquiries, but wondered if any on this list were aware of available technology to do this.
cheers, Rosemary
Dr Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 61 (0)2-6246 5475 GPO Box 1600 fax. 61 (0)2-6246 5334 Canberra, ACT 2601 Australia
==============================Original Headers============================== 5, 22 -- From Rosemary.White-at-csiro.au Thu Jun 7 18:06:13 2007 5, 22 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au [150.229.7.37]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57N6BUp006428 5, 22 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 18:06:12 -0500 5, 22 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=B/719hCOmj70x4fw8Xa9jlsNCiQ81UNL/KVQyRNlI1roNRlDs0bdsuAYvhHWzstYOtqiQHorAT8msXRgrzpp2byXutq6Cr6KK0FIId20OL2fZ/pEb92ipjpFWEHFSiHv; 5, 22 -- X-IronPort-AV: E=Sophos;i="4.16,397,1175436000"; 5, 22 -- d="scan'208";a="162041683" 5, 22 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) 5, 22 -- by act-ironport-int.csiro.au with ESMTP; 08 Jun 2007 09:06:03 +1000 5, 22 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 5, 22 -- Fri, 8 Jun 2007 09:06:10 +1000 5, 22 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 5, 22 -- Date: Fri, 08 Jun 2007 09:09:44 +1000 5, 22 -- Subject: fibre-optic probes/cameras? 5, 22 -- From: Rosemary White {Rosemary.White-at-csiro.au} 5, 22 -- To: {microscopy-at-microscopy.com} 5, 22 -- Message-ID: {C28ECD58.1DFA3%Rosemary.White-at-csiro.au} 5, 22 -- In-Reply-To: {200705311420.l4VEKqBb024233-at-ns.microscopy.com} 5, 22 -- Mime-version: 1.0 5, 22 -- Content-type: text/plain; charset="US-ASCII" 5, 22 -- Content-transfer-encoding: 7bit 5, 22 -- X-OriginalArrivalTime: 07 Jun 2007 23:06:10.0382 (UTC) FILETIME=[6FF3E6E0:01C7A958] ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both robby2-at-asu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: robby2-at-asu.edu Name: Robert W. Roberson
Organization: Arizona State University
Title-Subject: [Filtered] Position Available
Question: Research Scientist
An individual is sought, at the PhD level, who is skilled in methods of light and electron microscopy. He or she will play a key bioimaging role in a recently funded project for the development of biofuels through the manipulation of the cyanobacterium Synechocystis sp. PCC 6803. The individual will interact closely with a group of investigators within the School of Life Science and Biodesign Institute at Arizona State University. Specifically, the individual will be responsible for designing and implementing suitable protocols for microscopic analysis of strains that have been genetically modified and/or grown under conditions that favor the over production of fatty acids. The successful candidate will be able to perform basic and advanced bioimaging protocols such as: confocal microscopy, cryofixation and freeze substitution, ultramicrotomy, standard TEM and SEM imaging and electron tomography, and immuno-cytochemistry at both the light and EM levels. The School of Life Sciences Bioimaging Facility (http://sols.asu.edu/klab/index.php; http://sols.asu.edu/lsem/index.php) and associated imaging facilities at Arizona State University maintain the equipment required to fulfill the goals of the project.
Contact: Dr. Robert Roberson School of Life Sciences PO Box874501 Arizona State University Tempe, AZ 85287-4501
I've used different sized boroscopes before, like the ones at www.uxr.com (I have no affiliation with this company, I've just seen their products in action before)
I would say that a small boroscope would be your best bet, as you can port it out to video, and some come with a light source, but others may need a separate light source. I know they make both a flexible and a rigid boroscope, the rigid one being slightly less expensive.
What is the size diameter you are looking for?
--Justin A. Kraft
On 6/7/07, Rosemary.White-at-csiro.au {Rosemary.White-at-csiro.au} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } } Are there any really tiny cameras about, or at least, fibre-optic } lenses/objectives? I ask because we have a couple of applications that } could use one. We're interested in roots growing down pre-existing soil } pores, and would like to watch what they're doing down there, either with a } moving imaging device of some kind, or a series of portholes to watch roots } growing past. Another group is screening different lines of cereals, in } particular, looking at development of the floral meristem. This object of } desire is enclosed in many leaves at ground level in a cereal plant, we'd } like to poke a small fibre-optic in to watch its development over 3-6 days, } rather than having to destroy many plants to follow the stages of } development. } } I've had a rummage through numerous websites, and sent off some email } enquiries, but wondered if any on this list were aware of available } technology to do this. } } cheers, } Rosemary } } Dr Rosemary White rosemary.white-at-csiro.au } CSIRO Plant Industry ph. 61 (0)2-6246 5475 } GPO Box 1600 fax. 61 (0)2-6246 5334 } Canberra, ACT 2601 } Australia } } ==============================Original Headers============================== } 5, 22 -- From Rosemary.White-at-csiro.au Thu Jun 7 18:06:13 2007 } 5, 22 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au [150.229.7.37]) } 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57N6BUp006428 } 5, 22 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 18:06:12 -0500 } 5, 22 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=B/719hCOmj70x4fw8Xa9jlsNCiQ81UNL/KVQyRNlI1roNRlDs0bdsuAYvhHWzstYOtqiQHorAT8msXRgrzpp2byXutq6Cr6KK0FIId20OL2fZ/pEb92ipjpFWEHFSiHv; } 5, 22 -- X-IronPort-AV: E=Sophos;i="4.16,397,1175436000"; } 5, 22 -- d="scan'208";a="162041683" } 5, 22 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) } 5, 22 -- by act-ironport-int.csiro.au with ESMTP; 08 Jun 2007 09:06:03 +1000 } 5, 22 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); } 5, 22 -- Fri, 8 Jun 2007 09:06:10 +1000 } 5, 22 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 } 5, 22 -- Date: Fri, 08 Jun 2007 09:09:44 +1000 } 5, 22 -- Subject: fibre-optic probes/cameras? } 5, 22 -- From: Rosemary White {Rosemary.White-at-csiro.au} } 5, 22 -- To: {microscopy-at-microscopy.com} } 5, 22 -- Message-ID: {C28ECD58.1DFA3%Rosemary.White-at-csiro.au} } 5, 22 -- In-Reply-To: {200705311420.l4VEKqBb024233-at-ns.microscopy.com} } 5, 22 -- Mime-version: 1.0 } 5, 22 -- Content-type: text/plain; charset="US-ASCII" } 5, 22 -- Content-transfer-encoding: 7bit } 5, 22 -- X-OriginalArrivalTime: 07 Jun 2007 23:06:10.0382 (UTC) FILETIME=[6FF3E6E0:01C7A958] } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 29 -- From kraftpiano-at-gmail.com Thu Jun 7 18:43:38 2007 5, 29 -- Received: from wa-out-1112.google.com (wa-out-1112.google.com [209.85.146.179]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57NhbbJ029818 5, 29 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 18:43:37 -0500 5, 29 -- Received: by wa-out-1112.google.com with SMTP id v27so873009wah 5, 29 -- for {microscopy-at-microscopy.com} ; Thu, 07 Jun 2007 16:43:37 -0700 (PDT) 5, 29 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 5, 29 -- d=gmail.com; s=beta; 5, 29 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:cc:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 5, 29 -- b=ZMktC4Ijr/TBvOgIWjQAwJtSx5txDM8jd8jch/gnr6PixoqAo9MxT/HL8wsdtBREW+HSIMUO1B62JAhJJE2MEXT/El98Eq55eghVFW+D2wFYqf4+kx9GMqs0MEfWugYn2FUjfYFtt66VvovSuc0km0RBRBZPhBp0FuppKgYHOtI= 5, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 29 -- d=gmail.com; s=beta; 5, 29 -- h=received:message-id:date:from:to:subject:cc:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 5, 29 -- b=hPC2cFzenkRaqHdnSplzfRJ2/lHeMsTcOrM6KlsibZeA4nJutLKl6UI1vBhNYsrdPr1xBkE/byUR9jF8uGryKUteGA45HIK/ZLJd73wxQ70m8WVSc3P6NS2RXFUTXUQ0H4xFAtCBvYZEw38j3ADfQeUTSif61fd6nACH++3HroU= 5, 29 -- Received: by 10.115.90.1 with SMTP id s1mr2016369wal.1181259816159; 5, 29 -- Thu, 07 Jun 2007 16:43:36 -0700 (PDT) 5, 29 -- Received: by 10.114.92.13 with HTTP; Thu, 7 Jun 2007 16:43:36 -0700 (PDT) 5, 29 -- Message-ID: {25e2b0d20706071643l4e733e0fse755981ee390bf4-at-mail.gmail.com} 5, 29 -- Date: Thu, 7 Jun 2007 19:43:36 -0400 5, 29 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 5, 29 -- To: Rosemary.White-at-csiro.au 5, 29 -- Subject: Re: [Microscopy] fibre-optic probes/cameras? 5, 29 -- Cc: microscopy-at-microscopy.com 5, 29 -- In-Reply-To: {200706072314.l57NEo81017021-at-ns.microscopy.com} 5, 29 -- MIME-Version: 1.0 5, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 29 -- Content-Transfer-Encoding: 7bit 5, 29 -- Content-Disposition: inline 5, 29 -- References: {200706072314.l57NEo81017021-at-ns.microscopy.com} ==============================End of - Headers==============================
Hi Rosemary! Have you contacted Optiscan? They have some pretty small endoscopic microscopy devices which are really powerful. I'm sure they'd be as good for your application as they are for animal work. www.optiscan.com
Cheers, Roseys
I have no affiliation with Optiscan, just impressed by the demos I've seen
Rosemary van Driel Electron Microscopy New and Emerging Zoonotic Diseases Australian Animal Health Laboratory CSIRO Livestock Industries Private Bag 24 Geelong Vic 3220 Australia
International Phone: +61 3 5227 5209 International Fax: +61 3 5227 5555 email: Rosey.VanDriel-at-csiro.au
-----Original Message----- X-from: White, Rosemary (PI, Black Mountain) Sent: Friday, 8 June 2007 09:08 To: Van Driel, Rosey (LI, Geelong)
Dear all,
Are there any really tiny cameras about, or at least, fibre-optic lenses/objectives? I ask because we have a couple of applications that could use one. We're interested in roots growing down pre-existing soil pores, and would like to watch what they're doing down there, either with a moving imaging device of some kind, or a series of portholes to watch roots growing past. Another group is screening different lines of cereals, in particular, looking at development of the floral meristem. This object of desire is enclosed in many leaves at ground level in a cereal plant, we'd like to poke a small fibre-optic in to watch its development over 3-6 days, rather than having to destroy many plants to follow the stages of development.
I've had a rummage through numerous websites, and sent off some email enquiries, but wondered if any on this list were aware of available technology to do this.
cheers, Rosemary
Dr Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 61 (0)2-6246 5475 GPO Box 1600 fax. 61 (0)2-6246 5334 Canberra, ACT 2601 Australia
==============================Original Headers============================== 5, 22 -- From Rosemary.White-at-csiro.au Thu Jun 7 18:06:13 2007 5, 22 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au [150.229.7.37]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57N6BUp006428 5, 22 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 18:06:12 -0500 5, 22 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=B/719hCOmj70x4fw8Xa9jlsNCiQ81UNL/KVQyRNlI1roNRlDs0bdsuAYvhHWzstYOtqiQH orAT8msXRgrzpp2byXutq6Cr6KK0FIId20OL2fZ/pEb92ipjpFWEHFSiHv; 5, 22 -- X-IronPort-AV: E=Sophos;i="4.16,397,1175436000"; 5, 22 -- d="scan'208";a="162041683" 5, 22 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) 5, 22 -- by act-ironport-int.csiro.au with ESMTP; 08 Jun 2007 09:06:03 +1000 5, 22 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 5, 22 -- Fri, 8 Jun 2007 09:06:10 +1000 5, 22 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 5, 22 -- Date: Fri, 08 Jun 2007 09:09:44 +1000 5, 22 -- Subject: fibre-optic probes/cameras? 5, 22 -- From: Rosemary White {Rosemary.White-at-csiro.au} 5, 22 -- To: {microscopy-at-microscopy.com} 5, 22 -- Message-ID: {C28ECD58.1DFA3%Rosemary.White-at-csiro.au} 5, 22 -- In-Reply-To: {200705311420.l4VEKqBb024233-at-ns.microscopy.com} 5, 22 -- Mime-version: 1.0 5, 22 -- Content-type: text/plain; charset="US-ASCII" 5, 22 -- Content-transfer-encoding: 7bit 5, 22 -- X-OriginalArrivalTime: 07 Jun 2007 23:06:10.0382 (UTC) FILETIME=[6FF3E6E0:01C7A958] ==============================End of - Headers==============================
==============================Original Headers============================== 22, 31 -- From Rosey.VanDriel-at-csiro.au Thu Jun 7 18:50:27 2007 22, 31 -- Received: from vic-MTAout6.csiro.au (vic-MTAout6.csiro.au [150.229.64.43]) 22, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l57NoQc2008909 22, 31 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 18:50:26 -0500 22, 31 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=WfNS2mgwhJwqdlpxH1z4ftNNa/glfAqKsHgIBZlQyh3MJ2ueFFVCIOoozd9LtNrX1HVd7tc0UcpR42VtEgnkeir7XPncNrCP2N6dVrADO0CSQXo1wFLMTbE+3je8+KuU; 22, 31 -- X-IronPort-AV: E=Sophos;i="4.16,397,1175436000"; 22, 31 -- d="scan'208";a="137883350" 22, 31 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 22, 31 -- by vic-ironport-int.csiro.au with ESMTP; 08 Jun 2007 09:50:25 +1000 22, 31 -- Received: from exvicn1-mel.nexus.csiro.au ([138.194.3.60]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 22, 31 -- Fri, 8 Jun 2007 09:50:25 +1000 22, 31 -- Received: from EXVIC4-GEX.nexus.csiro.au ([138.194.208.10]) by exvicn1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 22, 31 -- Fri, 8 Jun 2007 09:50:25 +1000 22, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 22, 31 -- content-class: urn:content-classes:message 22, 31 -- MIME-Version: 1.0 22, 31 -- Content-Type: text/plain; 22, 31 -- charset="US-ASCII" 22, 31 -- Subject: RE: [Microscopy] fibre-optic probes/cameras? 22, 31 -- Date: Fri, 8 Jun 2007 09:49:08 +1000 22, 31 -- Message-ID: {8BE5C4878E8768478169A50D385160522E36BE-at-exvic4-gex.nexus.csiro.au} 22, 31 -- X-MS-Has-Attach: 22, 31 -- X-MS-TNEF-Correlator: 22, 31 -- Thread-Topic: [Microscopy] fibre-optic probes/cameras? 22, 31 -- Thread-Index: AcepWLV0lhqD+qj/RqmDOW5ZKM2bswABFxnQ 22, 31 -- References: {200706072308.l57N834Y009222-at-ns.microscopy.com} 22, 31 -- From: {Rosey.VanDriel-at-csiro.au} 22, 31 -- To: {Rosemary.White-at-csiro.au} , {microscopy-at-microscopy.com} 22, 31 -- X-OriginalArrivalTime: 07 Jun 2007 23:50:25.0038 (UTC) FILETIME=[9E4042E0:01C7A95E] 22, 31 -- Content-Transfer-Encoding: 8bit 22, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l57NoQc2008909 ==============================End of - Headers==============================
The diameter - as small as possible..... ideally 1mm or less, which I know is smaller than most catheter imaging systems, for example. However, for the root project, the boroscopes look pretty good. And of course, I got more web results using fiber-optics rather than fibre-optics.....the boroscope site didn¹t show up before... thanks, cheers, Rosemary
} From: "Justin Kraft" {kraftpiano-at-gmail.com} } Date: Thu, 7 Jun 2007 19:43:36 -0400 } To: Rosemary.White-at-csiro.au } Cc: microscopy-at-microscopy.com } Subject: Re: [Microscopy] fibre-optic probes/cameras? } } I've used different sized boroscopes before, like the ones at } www.uxr.com (I have no affiliation with this company, I've just seen } their products in action before) } } I would say that a small boroscope would be your best bet, as you can } port it out to video, and some come with a light source, but others } may need a separate light source. I know they make both a flexible } and a rigid boroscope, the rigid one being slightly less expensive. } } What is the size diameter you are looking for? } } --Justin A. Kraft } } On 6/7/07, Rosemary.White-at-csiro.au {Rosemary.White-at-csiro.au} wrote: } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear all, } } } } Are there any really tiny cameras about, or at least, fibre-optic } } lenses/objectives? I ask because we have a couple of applications that } } could use one. We're interested in roots growing down pre-existing soil } } pores, and would like to watch what they're doing down there, either with a } } moving imaging device of some kind, or a series of portholes to watch roots } } growing past. Another group is screening different lines of cereals, in } } particular, looking at development of the floral meristem. This object of } } desire is enclosed in many leaves at ground level in a cereal plant, we'd } } like to poke a small fibre-optic in to watch its development over 3-6 days, } } rather than having to destroy many plants to follow the stages of } } development. } } } } I've had a rummage through numerous websites, and sent off some email } } enquiries, but wondered if any on this list were aware of available } } technology to do this. } } } } cheers, } } Rosemary } } } } Dr Rosemary White rosemary.white-at-csiro.au } } CSIRO Plant Industry ph. 61 (0)2-6246 5475 } } GPO Box 1600 fax. 61 (0)2-6246 5334 } } Canberra, ACT 2601 } } Australia } } } } ==============================Original Headers============================== } } 5, 22 -- From Rosemary.White-at-csiro.au Thu Jun 7 18:06:13 2007 } } 5, 22 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au } } [150.229.7.37]) } } 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } l57N6BUp006428 } } 5, 22 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 18:06:12 } } -0500 } } 5, 22 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; } } b=B/719hCOmj70x4fw8Xa9jlsNCiQ81UNL/KVQyRNlI1roNRlDs0bdsuAYvhHWzstYOtqiQHorAT8 } } msXRgrzpp2byXutq6Cr6KK0FIId20OL2fZ/pEb92ipjpFWEHFSiHv; } } 5, 22 -- X-IronPort-AV: E=Sophos;i="4.16,397,1175436000"; } } 5, 22 -- d="scan'208";a="162041683" } } 5, 22 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) } } 5, 22 -- by act-ironport-int.csiro.au with ESMTP; 08 Jun 2007 09:06:03 } } +1000 } } 5, 22 -- Received: from [152.83.167.45] ([152.83.167.45]) by } } exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); } } 5, 22 -- Fri, 8 Jun 2007 09:06:10 +1000 } } 5, 22 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 } } 5, 22 -- Date: Fri, 08 Jun 2007 09:09:44 +1000 } } 5, 22 -- Subject: fibre-optic probes/cameras? } } 5, 22 -- From: Rosemary White {Rosemary.White-at-csiro.au} } } 5, 22 -- To: {microscopy-at-microscopy.com} } } 5, 22 -- Message-ID: {C28ECD58.1DFA3%Rosemary.White-at-csiro.au} } } 5, 22 -- In-Reply-To: {200705311420.l4VEKqBb024233-at-ns.microscopy.com} } } 5, 22 -- Mime-version: 1.0 } } 5, 22 -- Content-type: text/plain; charset="US-ASCII" } } 5, 22 -- Content-transfer-encoding: 7bit } } 5, 22 -- X-OriginalArrivalTime: 07 Jun 2007 23:06:10.0382 (UTC) } } FILETIME=[6FF3E6E0:01C7A958] } } ==============================End of - Headers============================== } } }
==============================Original Headers============================== 3, 26 -- From Rosemary.White-at-csiro.au Thu Jun 7 19:22:22 2007 3, 26 -- Received: from act-MTAout6.csiro.au (act-MTAout6.csiro.au [150.229.7.43]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l580MLwT020803 3, 26 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 19:22:21 -0500 3, 26 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=Sd9ytDUi7g/wtl0fwJPRyW5eLlwis3Fnc9taQvk1kgIW9PEahRmbB5TMEfJT/JOt9NhcKnif65t3ytsp/1dqedCjyUVwy9PIQ9sArUnnGha5Yi1Kb9RoqwgZ/F/iUP9T; 3, 26 -- X-IronPort-AV: E=Sophos;i="4.16,397,1175436000"; 3, 26 -- d="scan'208";a="162049929" 3, 26 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 3, 26 -- by act-ironport-int.csiro.au with ESMTP; 08 Jun 2007 10:22:11 +1000 3, 26 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 3, 26 -- Fri, 8 Jun 2007 10:22:14 +1000 3, 26 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 3, 26 -- Fri, 8 Jun 2007 10:22:14 +1000 3, 26 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 3, 26 -- Date: Fri, 08 Jun 2007 10:25:48 +1000 3, 26 -- Subject: Re: [Microscopy] fibre-optic probes/cameras? 3, 26 -- From: Rosemary White {Rosemary.White-at-csiro.au} 3, 26 -- To: Justin Kraft {kraftpiano-at-gmail.com} 3, 26 -- CC: {microscopy-at-microscopy.com} 3, 26 -- Message-ID: {C28EDF2C.1DFC9%Rosemary.White-at-csiro.au} 3, 26 -- In-Reply-To: {25e2b0d20706071643l4e733e0fse755981ee390bf4-at-mail.gmail.com} 3, 26 -- Mime-version: 1.0 3, 26 -- Content-type: text/plain; charset="ISO-8859-1" 3, 26 -- X-OriginalArrivalTime: 08 Jun 2007 00:22:14.0290 (UTC) FILETIME=[1040FF20:01C7A963] 3, 26 -- Content-Transfer-Encoding: 8bit 3, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l580MLwT020803 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tstrixnerharvey-at-qmag.com.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Purchasing an entry level SEM
Question: Good Morning Everyone,
I have been given the task of purchasing an "entry level" SEM capable of doing surface analaysis and elemental mapping of mineralogical samples.
Unfortunately may knowledge of SEM extends to sending samples away to a university and getting beautiful reports and photographs back.
What I need is an instrument able to do EDS measurements of light elements Z=11 and above. My samples need to be carbon coated and have an approximate minimum crystal size of 5 micron.
I want to use the instrument to look at gross crystal structures (at 5 micron) such as nicely layered or random etc.
Also I want to do elemental mapping of different products at larger crystal sizes (30-5000um).
I have a reasonable budget but not extensive.
I would appreciate some pointers.
Thank you kindly.
Kind Regards,
Tania Strixner-Harvey Principal - Research and Development
Queensland Magnesia 246 Boundary Road Parkhurst, 4702 Australia
X-from what you describe, you need not an "entry level" SEM, but a conventional SEM (high vacuum, W filament) and a reasonable EDS with high count rate and software to be able to do mapping.
I have JEOL 6400 with Oxford INCA EDS, it comfortably does what you describe. The only complication is that the largest map it can do at a time is about 6 mm. So, to acquire an elemental map of 5 mm crystals and have several of them in the field of view you will also need a motorised stage controlled by the EDS software. An alternative would be to collect several maps and stitch them together.
You will also need to budget for some cutting/polishing equipment a carbon coater.
Alex
============== Alexander Titkov Senior Scientist Millennium Inorganic Chemicals a Cristal Company Lot 4 Old Coast Road Australind WA 6233 AUSTRALIA
tstrixnerharvey-at-q mag.com.au To: alex.titkov-at-millenniumchem.com cc: 08/06/2007 08:38 Subject: [Microscopy] viaWWW: Purchasing an entry level SEM AM Please respond to tstrixnerharvey
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tstrixnerharvey-at-qmag.com.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Purchasing an entry level SEM
Question: Good Morning Everyone,
I have been given the task of purchasing an "entry level" SEM capable of doing surface analaysis and elemental mapping of mineralogical samples.
Unfortunately may knowledge of SEM extends to sending samples away to a university and getting beautiful reports and photographs back.
What I need is an instrument able to do EDS measurements of light elements Z=11 and above. My samples need to be carbon coated and have an approximate minimum crystal size of 5 micron.
I want to use the instrument to look at gross crystal structures (at 5 micron) such as nicely layered or random etc.
Also I want to do elemental mapping of different products at larger crystal sizes (30-5000um).
I have a reasonable budget but not extensive.
I would appreciate some pointers.
Thank you kindly.
Kind Regards,
Tania Strixner-Harvey Principal - Research and Development
Queensland Magnesia 246 Boundary Road Parkhurst, 4702 Australia
==============================Original Headers ============================== 16, 11 -- From zaluzec-at-microscopy.com Thu Jun 7 19:36:01 2007 16, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 16, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l580Zx4V032694 16, 11 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 19:36:00 -0500 16, 11 -- Mime-Version: 1.0 16, 11 -- Message-Id: {p06240802c28e54de9ab7-at-[206.69.208.22]} 16, 11 -- Date: Thu, 7 Jun 2007 19:35:59 -0500 16, 11 -- To: microscopy-at-microscopy.com 16, 11 -- From: tstrixnerharvey-at-qmag.com.au (by way of MicroscopyListserver) 16, 11 -- Subject: viaWWW: Purchasing an entry level SEM 16, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers ==============================
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==============================Original Headers============================== 49, 23 -- From alex.titkov-at-millenniumchem.com Thu Jun 7 20:36:53 2007 49, 23 -- Received: from edcpmp01.lyondell.com (mail4.lyondell.com [161.16.0.78]) 49, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l581ar9f012984 49, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Jun 2007 20:36:53 -0500 49, 23 -- Received: from edcexp01.lyondell.com ([161.16.151.68]) 49, 23 -- by edcpap02.lyondell.com (8.13.7/8.13.7) with ESMTP id l581aqG3020793; 49, 23 -- Thu, 7 Jun 2007 20:36:52 -0500 49, 23 -- Received: from amnhhtv01.millenniumchem.com ([172.19.0.46]) by edcexp01.lyondell.com with Microsoft SMTPSVC(5.0.2195.6713); 49, 23 -- Thu, 7 Jun 2007 20:36:51 -0500 49, 23 -- Subject: Re: [Microscopy] viaWWW: Purchasing an entry level SEM 49, 23 -- To: tstrixnerharvey-at-qmag.com.au 49, 23 -- Cc: Microscopy-at-microscopy.com 49, 23 -- X-Mailer: Lotus Notes Release 5.0.11 July 24, 2002 49, 23 -- Message-ID: {OFAC77D30E.868B4AF1-ONC82572F4.000639C3-C82572F4.0008BBDD-at-millenniumchem.com} 49, 23 -- From: alex.titkov-at-millenniumchem.com 49, 23 -- Date: Fri, 8 Jun 2007 10:35:14 +0900 49, 23 -- X-MIMETrack: Serialize by Router on AMNHHTV01/HUB/SRV/MIC(Release 5.0.11 |July 24, 2002) at 49, 23 -- 06/07/2007 09:36:51 PM 49, 23 -- MIME-Version: 1.0 49, 23 -- Content-type: text/plain; charset=us-ascii 49, 23 -- X-OriginalArrivalTime: 08 Jun 2007 01:36:51.0757 (UTC) FILETIME=[7D083DD0:01C7A96D] 49, 23 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=3.1.0-0705030000 definitions=main-0706070102 49, 23 -- X-Proofpoint-OriginalSender: alex.titkov-at-millenniumchem.com ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (ardnek2-at-att.net ) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, June 7, 2007 at 21:03:50 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both ardnek2-at-att.net as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: ardnek2-at-att.net Name: Kendra Orr
Organization: ARDNEK TUTORING
Education: 6-8th Grade Middle School
Location: Fort Lauderdale, FL 33334
Title: Re: Syringes with spores
Question: This may seem like a stupid question. However, I want to be precise. May I order a spore syringe and it will spit out spores tiny enough to see on a microscope? I am sorry........I am doing a presentation and I usually use spores from real plants that I cultivate.
Do you or anyone else know if a large fiber optic strand of .25 to 1.0 mm can conduct an image that can be viewed with am 20x to 50x microscope objective on the polished end of the fiber away form the subject in this case roots.
I believe a lens could melted on the other end of a single fiber and computer software could correct the distortions.
In a project such are yours I would think having a great number of low cost lenses wold give much better coverage and allow you to cut years of the time it takes to get results.
Gordon Couger Stillwater OK 405 624 2855 Rosemary.White-at-csiro.au wrote: } } The diameter - as small as possible..... ideally 1mm or less, which I know } is smaller than most catheter imaging systems, for example. However, for } the root project, the boroscopes look pretty good. } And of course, I got more web results using fiber-optics rather than } fibre-optics.....the boroscope site didn¹t show up before... } thanks, } cheers, } Rosemary } } } } From: "Justin Kraft" {kraftpiano-at-gmail.com} } } Date: Thu, 7 Jun 2007 19:43:36 -0400 } } To: Rosemary.White-at-csiro.au } } Cc: microscopy-at-microscopy.com } } Subject: Re: [Microscopy] fibre-optic probes/cameras? } } } } I've used different sized boroscopes before, like the ones at } } www.uxr.com (I have no affiliation with this company, I've just seen } } their products in action before) } } } } I would say that a small boroscope would be your best bet, as you can } } port it out to video, and some come with a light source, but others } } may need a separate light source. I know they make both a flexible } } and a rigid boroscope, the rigid one being slightly less expensive. } } } } What is the size diameter you are looking for? } } } } --Justin A. Kraft } } } } On 6/7/07, Rosemary.White-at-csiro.au {Rosemary.White-at-csiro.au} wrote: } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Dear all, } } } } } } Are there any really tiny cameras about, or at least, fibre-optic } } } lenses/objectives? I ask because we have a couple of applications that } } } could use one. We're interested in roots growing down pre-existing soil } } } pores, and would like to watch what they're doing down there, either with a } } } moving imaging device of some kind, or a series of portholes to watch roots } } } growing past. Another group is screening different lines of cereals, in } } } particular, looking at development of the floral meristem. This object of } } } desire is enclosed in many leaves at ground level in a cereal plant, we'd } } } like to poke a small fibre-optic in to watch its development over 3-6 days, } } } rather than having to destroy many plants to follow the stages of } } } development. } } } } } } I've had a rummage through numerous websites, and sent off some email } } } enquiries, but wondered if any on this list were aware of available } } } technology to do this. } } } } } } cheers, } } } Rosemary } } } } } } Dr Rosemary White rosemary.white-at-csiro.au } } } CSIRO Plant Industry ph. 61 (0)2-6246 5475 } } } GPO Box 1600 fax. 61 (0)2-6246 5334 } } } Canberra, ACT 2601 } } } Australia }
==============================Original Headers============================== 8, 20 -- From gcouger-at-science-info.net Fri Jun 8 02:50:52 2007 8, 20 -- Received: from smtp106.biz.mail.re2.yahoo.com (smtp106.biz.mail.re2.yahoo.com [206.190.52.175]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l587opaR009827 8, 20 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jun 2007 02:50:51 -0500 8, 20 -- Received: (qmail 30482 invoked from network); 8 Jun 2007 07:50:51 -0000 8, 20 -- Received: from unknown (HELO ?192.168.1.100?) (gcouger-at-science-info.net-at-74.195.225.38 with plain) 8, 20 -- by smtp106.biz.mail.re2.yahoo.com with SMTP; 8 Jun 2007 07:50:50 -0000 8, 20 -- X-YMail-OSG: YzK7UdQVM1kexYzHUc3hxlL1LTVHQrZshdzxUN5jleVa_xvSqaiVD0pV4gmheHGNHLKJ2kmKz6gAZvF_p1GuvTeiSn_bctFCdeVoI2R_areKwJxhkL15O4VbmIxwcZV6vJSinAA0jydeaw-- 8, 20 -- Message-ID: {46690A5F.3030508-at-science-info.net} 8, 20 -- Date: Fri, 08 Jun 2007 02:50:55 -0500 8, 20 -- From: Gordon Couger {gcouger-at-science-info.net} 8, 20 -- User-Agent: Thunderbird 1.5.0.12 (Macintosh/20070509) 8, 20 -- MIME-Version: 1.0 8, 20 -- To: Rosemary.White-at-csiro.au, microscopy-at-microscopy.com 8, 20 -- Subject: Re: [Microscopy] Re: fibre-optic probes/cameras? 8, 20 -- References: {200706080027.l580RXK4031395-at-ns.microscopy.com} 8, 20 -- In-Reply-To: {200706080027.l580RXK4031395-at-ns.microscopy.com} 8, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l587opaR009827 ==============================End of - Headers==============================
Been not involved in carbon nanotube research I dont know exactly how dense they are under the beam of a TEM. However I don't think you can compare the density of the carbon present in a tissue with the density of carbon in nanotubes. The difference MUST be visible. I wouldn't modify the nanotubes themselves because you will definitely modify their behaviour in an uncontrolled manner. Now here is what I would do: I would incubate cell monolayers with the nanoparticles and flat embed them (control=cells incubated at 4°C, no uptake) after ferrocyanide-osmium post-fixation. I am pretty sure the cells will internalize the particles (take hepatocytes like HepG2 they are very effecient at uptaking and very nice to show). After sectionning, I would try different contrasting intensities and also without any contrasting at all! This way you can define your technical parameters to obtain the best contrast possible in "control" samples.
Finding these particles in organs is another story, requiring mainly eternities of patience and dedication. But, if I may give my personal opinion, this is a very interesting study and probably a very rewarding one. Few have had the heart to start it.
Regards and good luck,
Stephane
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==============================Original Headers============================== 9, 21 -- From nizets2-at-yahoo.com Fri Jun 8 05:52:37 2007 9, 21 -- Received: from web37414.mail.mud.yahoo.com (web37414.mail.mud.yahoo.com [209.191.91.146]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l58AqbJR028188 9, 21 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jun 2007 05:52:37 -0500 9, 21 -- Received: (qmail 36206 invoked by uid 60001); 8 Jun 2007 10:52:36 -0000 9, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 21 -- s=s1024; d=yahoo.com; 9, 21 -- h=X-YMail-OSG:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 9, 21 -- b=Th7IcHvEcm38JIhX3/l26JoxXFwymDMC5uDRN8g0oDgryha87yhpMfJ/YBB3aZl53Kro1J40P1SnNaaTGMit9wjcHw7nvSn5v5BIxymLkWVXs5jyL7V8hErX+l/+MtxBhwe06vj+BH2EZGakbfTnLz31RCHy/vxSBATSehs5b3I=; 9, 21 -- X-YMail-OSG: qHwdNQIVM1lbwnLKYcwm.jWyeXsF73stJh0XXkQWKZ6kiL20PdHKKfrhkFYFI4QjxALnY19L6V.hfcebfLhRZ5yi9pXY0oEjSCUlvJ3o7Ruc7M6H3ns- 9, 21 -- Received: from [80.122.101.102] by web37414.mail.mud.yahoo.com via HTTP; Fri, 08 Jun 2007 03:52:36 PDT 9, 21 -- Date: Fri, 8 Jun 2007 03:52:36 -0700 (PDT) 9, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 9, 21 -- Subject: Re: [Microscopy] RE: Carbon nanos in tissue: SEM/TEM 9, 21 -- To: TindallR-at-missouri.edu 9, 21 -- Cc: microscopy-at-microscopy.com 9, 21 -- In-Reply-To: {200706071700.l57H0ZUv016579-at-ns.microscopy.com} 9, 21 -- MIME-Version: 1.0 9, 21 -- Content-Type: text/plain; charset=iso-8859-1 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- Message-ID: {674274.31149.qm-at-web37414.mail.mud.yahoo.com} ==============================End of - Headers==============================
Dear Tania, You should consider a basic variable-pressure SEM. They are very good for mineralogical work and have the added bonus of not requiring a carbon coating. For what you want to do, the new, simple table-top SEMs, such as the Hitachi TM-1000 or the FEI Phenom-Ed might be ideal, if they do EDS. They are very fast, simple and low cost. They use BSE imaging, which shows up the grains on polished minerals very well. Otherwise , a simple, low-cost variable-pressure SEM will be your best bet. I didn't think that the variable-pressure SEM's had much to offer materials engineering applications, but we are using it all the time and finding new uses for it every day. BTW, all the modern EDS systems will do the elements Z=5 (boron) and above. Regards,
-----Original Message----- X-from: tstrixnerharvey-at-qmag.com.au [mailto:tstrixnerharvey-at-qmag.com.au] Sent: June 7, 2007 5:40 PM To: mager-at-interchange.ubc.ca
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Title-Subject: [Filtered] Purchasing an entry level SEM
Question: Good Morning Everyone,
I have been given the task of purchasing an "entry level" SEM capable of doing surface analaysis and elemental mapping of mineralogical samples.
Unfortunately may knowledge of SEM extends to sending samples away to a university and getting beautiful reports and photographs back.
What I need is an instrument able to do EDS measurements of light elements Z=11 and above. My samples need to be carbon coated and have an approximate minimum crystal size of 5 micron.
I want to use the instrument to look at gross crystal structures (at 5 micron) such as nicely layered or random etc.
Also I want to do elemental mapping of different products at larger crystal sizes (30-5000um).
I have a reasonable budget but not extensive.
I would appreciate some pointers.
Thank you kindly.
Kind Regards,
Tania Strixner-Harvey Principal - Research and Development
Queensland Magnesia 246 Boundary Road Parkhurst, 4702 Australia
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Question: I have a Cambridge SEM and I am trying to preform voltage contrast on a multi-layer ceramic capacitor. It has been well over 10 years since I've used VC and I must have forgotten something. I can blow them up, but can get them to light up. Does anyone have a guess on what I'm doing wrong. My parameters are 5 acceleration voltage. Power supply positive wire connected capacitor and negative connected to ground. SEM bias turned off. Thanks for any suggestions.
The May Archives for the Microscopy Listserver are now on-line at
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==============================Original Headers============================== 8, 11 -- From zaluzec-at-microscopy.com Sat Jun 9 11:49:38 2007 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l59Gnbxw012334 8, 11 -- for {microscopy-at-microscopy.com} ; Sat, 9 Jun 2007 11:49:38 -0500 8, 11 -- Mime-Version: 1.0 8, 11 -- Message-Id: {p0624080bc2908996a6f3-at-[206.69.208.22]} 8, 11 -- Date: Sat, 9 Jun 2007 11:49:37 -0500 8, 11 -- To: microscopy-at-microscopy.com 8, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 8, 11 -- Subject: Administrivia: May 2007 Archives on-line 8, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Johnson Matthey PLC is a specialist chemicals company focused on its core skills in catalysts, precious metals and fine chemicals. The Technology Centre, based at Sonning Common (approximately 40 miles West of London), undertakes research work for the group.
A vacancy has arisen at the Technology Centre for an Electron Microscopist to join our team working with both Scanning and Transmission microscopes.
The successful candidate is expected to have experience in several of the following areas of expertise:
SEM and TEM sample preparation: (Coating; Ultramicrotomy; Cryo-sample preparation) Electron Microscope technical knowledge: (FEG sources, vacuum systems; electronics; fault finding and diagnosis) SEM and TEM Characterisation methods: (HRTEM; STEM; HAADF Imaging; EDX; EELS; Electron diffraction analysis)
The job is based on the analysis of a variety of samples from our research groups and operating divisions, thus prior exposure to multidisciplinary subjects as well as catalysis and materials would be an advantage.
The successful candidate will be educated to HNC/Degree level as a minimum. Applications must be made in writing with full CV and current salary details to: Georgie Floyd, Personnel Officer, Johnson Matthey Technology Centre, Blounts Court, Sonning Common, Reading, RG4 9NH or e-mail hrjmtc-at-matthey.com
Closing date for applications: Friday 6th July 2007
If the reader of this email is not the intended recipient(s), please be advised that any dissemination, distribution or copying of this information is strictly prohibited. Johnson Matthey PLC has its main place of business at 40-42 Hatton Garden, London (020 7269 8400).
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Please note that your communication may be monitored in accordance with Johnson Matthey internal policy documentation.
==============================Original Headers============================== 18, 24 -- From goodlg-at-matthey.com Mon Jun 11 04:39:39 2007 18, 24 -- Received: from cluster-e.mailcontrol.com (cluster-e.mailcontrol.com [217.79.216.190]) 18, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5B9ddvn022539 18, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 04:39:39 -0500 18, 24 -- Received: from royhosmtp.pmp.royston.matthey.com ([194.202.191.212]) 18, 24 -- by rly08e.srv.mailcontrol.com (MailControl) with ESMTP id l5B9dYCc006082 18, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 10:39:35 +0100 18, 24 -- Received: from tsqhof6.trafalgar.jm ([192.168.1.238]) 18, 24 -- by royhosmtp.pmp.royston.matthey.com (8.13.6/8.13.6) with ESMTP id l5B9o7el009946 18, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 10:50:07 +0100 18, 24 -- Received: from London-MTA by tsqhof6.trafalgar.jm 18, 24 -- with Novell_GroupWise; Mon, 11 Jun 2007 10:39:34 +0100 18, 24 -- Message-Id: {s66d2666.019-at-tsqhof6.trafalgar.jm} 18, 24 -- X-Mailer: Novell GroupWise Internet Agent 6.5.4 18, 24 -- Date: Mon, 11 Jun 2007 10:38:59 +0100 18, 24 -- From: "Gregory Goodlet" {goodlg-at-matthey.com} 18, 24 -- To: {Microscopy-at-microscopy.com} 18, 24 -- Subject: SEM/TEM Position JM (U.K.) 18, 24 -- Mime-Version: 1.0 18, 24 -- Content-Type: text/plain; charset=US-ASCII 18, 24 -- Content-Disposition: inline 18, 24 -- X-Scanned-By: MailControl A-07-07-05 (www.mailcontrol.com) on 10.69.0.118 18, 24 -- Content-Transfer-Encoding: 8bit 18, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5B9ddvn022539 ==============================End of - Headers==============================
Hello all, I have been asked what the voltage and current conditions are for evaporation of aluminum in a tungsten basket in a vacuum evaporator. Is this sort of information available in a reference? I don't have much experience with metal evaporation, so any advice would be appreciated. Thanks, Kim
-- Kim Rensing Ph.D. Assistant Research Professor Manager, Microscopy and Imaging Facility
University of Calgary Health Sciences Centre B129 - 3330 Hospital Drive NW Calgary, AB, Canada T2N 4N1 403-220-3488 krensing-at-ucalgary.ca
==============================Original Headers============================== 4, 20 -- From krensing-at-ucalgary.ca Mon Jun 11 10:11:37 2007 4, 20 -- Received: from smtp3.ucalgary.ca (smtp3.ucalgary.ca [136.159.34.64]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5BFBbHO009211 4, 20 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 10:11:37 -0500 4, 20 -- Received: from [136.159.164.163] (unknown [136.159.164.163]) 4, 20 -- by smtp3.ucalgary.ca (Postfix) with ESMTP id 0AD5D4034 4, 20 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 09:11:31 -0600 (MDT) 4, 20 -- Message-ID: {466D661E.4080807-at-ucalgary.ca} 4, 20 -- Date: Mon, 11 Jun 2007 09:11:26 -0600 4, 20 -- From: Kim Rensing {krensing-at-ucalgary.ca} 4, 20 -- Organization: Microscopy and Imaging Facility, U. of Calgary 4, 20 -- User-Agent: Thunderbird 1.5.0.12 (Windows/20070509) 4, 20 -- MIME-Version: 1.0 4, 20 -- To: microscopy-at-microscopy.com 4, 20 -- Subject: aluminum evaporation 4, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 20 -- Content-Transfer-Encoding: 7bit 4, 20 -- X-UCalgary-MailScanner-Information: Please contact IT Help Desk at (403) 220-5555 for more information 4, 20 -- X-UCalgary-MailScanner: Found to be clean 4, 20 -- X-UCalgary-MailScanner-From: krensing-at-ucalgary.ca ==============================End of - Headers==============================
Can anyone recommend or otherwise provide information on an affordable(few thousand $) Peltier cold stage suitable for use in an SEM level vacuum (we will modify to fit several instruments)? If you know of suitable components, our machine shop can build or modify as needed. Thanks,
Dale Batchelor, Ph.D. Associate Director Analytical Instrumentation Facility N.C. State University Monteith Research Center room 318A Campus Box 7531 Raleigh, NC 27695 Office 919-515-3841 FAX 919-515-6965 E-Mail dale_batchelor-at-ncsu.edu Website www.ncsu.edu/aif
==============================Original Headers============================== 3, 19 -- From dale_batchelor-at-ncsu.edu Mon Jun 11 12:26:51 2007 3, 19 -- Received: from uni10mr.unity.ncsu.edu (uni10mr.unity.ncsu.edu [152.1.1.170]) 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5BHQpXn001010 3, 19 -- for {Microscopy-at-MSA.Microscopy.com} ; Mon, 11 Jun 2007 12:26:51 -0500 3, 19 -- Received: from [152.14.52.68] (dbmacx.aif.ncsu.edu [152.14.52.68]) 3, 19 -- by uni10mr.unity.ncsu.edu (8.13.7/8.13.8/Nv5.2006.1109) with ESMTP id l5BHQonb001347 3, 19 -- for {Microscopy-at-MSA.Microscopy.com} ; Mon, 11 Jun 2007 13:26:50 -0400 (EDT) 3, 19 -- Mime-Version: 1.0 (Apple Message framework v624) 3, 19 -- Content-Transfer-Encoding: 7bit 3, 19 -- Message-Id: {a0fb0d0d35f30cddf352e16f6bda9487-at-ncsu.edu} 3, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 3, 19 -- To: Microscopy-at-MSA.Microscopy.com 3, 19 -- From: Dale Batchelor {dale_batchelor-at-ncsu.edu} 3, 19 -- Subject: [Microscopy] SEM: Peltier Cooled Stage 3, 19 -- Date: Mon, 11 Jun 2007 13:28:21 -0400 3, 19 -- X-Mailer: Apple Mail (2.624) 3, 19 -- X-PMX-Version: 5.3.1.294258, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.6.11.100135 3, 19 -- X-Spam-Status: No, Hits=7% 3, 19 -- X-Spam-Level: IIIIIII ==============================End of - Headers==============================
Emitech has a Peltier Cold Stage for SEM, the K25X, however, you'll pay more than a few thousand dollars for the unit. Energy Beam Sciences is the US Master Distributor for Emitech Instruments, and we'd be happy to review your needs for this instrument with you.
Regards,
Mike Dufraine EM-Product Manager
dale_batchelor-at-ncsu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Can anyone recommend or otherwise provide information on an } affordable(few thousand $) Peltier cold stage suitable for use in an SEM } level vacuum (we will modify to fit several instruments)? If you know } of suitable components, our machine shop can build or modify as needed. } Thanks, } } Dale Batchelor, Ph.D. } Associate Director } Analytical Instrumentation Facility } N.C. State University } Monteith Research Center room 318A } Campus Box 7531 } Raleigh, NC 27695 } Office 919-515-3841 } FAX 919-515-6965 } E-Mail dale_batchelor-at-ncsu.edu } Website www.ncsu.edu/aif } } } ==============================Original Headers============================== } 3, 19 -- From dale_batchelor-at-ncsu.edu Mon Jun 11 12:26:51 2007 } 3, 19 -- Received: from uni10mr.unity.ncsu.edu (uni10mr.unity.ncsu.edu [152.1.1.170]) } 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5BHQpXn001010 } 3, 19 -- for {Microscopy-at-MSA.Microscopy.com} ; Mon, 11 Jun 2007 12:26:51 -0500 } 3, 19 -- Received: from [152.14.52.68] (dbmacx.aif.ncsu.edu [152.14.52.68]) } 3, 19 -- by uni10mr.unity.ncsu.edu (8.13.7/8.13.8/Nv5.2006.1109) with ESMTP id l5BHQonb001347 } 3, 19 -- for {Microscopy-at-MSA.Microscopy.com} ; Mon, 11 Jun 2007 13:26:50 -0400 (EDT) } 3, 19 -- Mime-Version: 1.0 (Apple Message framework v624) } 3, 19 -- Content-Transfer-Encoding: 7bit } 3, 19 -- Message-Id: {a0fb0d0d35f30cddf352e16f6bda9487-at-ncsu.edu} } 3, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed } 3, 19 -- To: Microscopy-at-MSA.Microscopy.com } 3, 19 -- From: Dale Batchelor {dale_batchelor-at-ncsu.edu} } 3, 19 -- Subject: [Microscopy] SEM: Peltier Cooled Stage } 3, 19 -- Date: Mon, 11 Jun 2007 13:28:21 -0400 } 3, 19 -- X-Mailer: Apple Mail (2.624) } 3, 19 -- X-PMX-Version: 5.3.1.294258, Antispam-Engine: 2.5.0.283055, Antispam-Data: 2007.6.11.100135 } 3, 19 -- X-Spam-Status: No, Hits=7% } 3, 19 -- X-Spam-Level: IIIIIII } ==============================End of - Headers============================== }
-- Michael F. Dufraine EM - Product Manager TEL 800-992-9037 X340 MDufraine-at-ebsciences.com www.ebsciences.com
==============================Original Headers============================== 7, 26 -- From mdufraine-at-ebsciences.com Mon Jun 11 12:43:04 2007 7, 26 -- Received: from mx-outbound01.easydns.com (mailout.easydns.com [205.210.42.54]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5BHh3Sj012725 7, 26 -- for {Microscopy-at-MSA.Microscopy.com} ; Mon, 11 Jun 2007 12:43:03 -0500 7, 26 -- Received: from mail.ebsciences.com (unknown [69.182.224.2]) 7, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 7, 26 -- (No client certificate requested) 7, 26 -- by mx-outbound01.easydns.com (Postfix) with ESMTP id 225417FD5; 7, 26 -- Mon, 11 Jun 2007 13:43:00 -0400 (EDT) 7, 26 -- Received: from mdufraine.ebsciences.private ([10.10.0.196]) 7, 26 -- by mail.ebsciences.com with esmtpsa (TLSv1:AES256-SHA:256) 7, 26 -- (Exim 4.67) 7, 26 -- (envelope-from {mdufraine-at-ebsciences.com} ) 7, 26 -- id 1Hxnuu-0004yg-AO; Mon, 11 Jun 2007 13:43:00 -0400 7, 26 -- Message-ID: {466D89A3.6000904-at-ebsciences.com} 7, 26 -- Date: Mon, 11 Jun 2007 13:42:59 -0400 7, 26 -- From: Mike Dufraine {mdufraine-at-ebsciences.com} 7, 26 -- Organization: Energy Beam Sciences 7, 26 -- User-Agent: Thunderbird 2.0.0.0 (Windows/20070326) 7, 26 -- MIME-Version: 1.0 7, 26 -- To: dale_batchelor-at-ncsu.edu, Microscopy-at-MSA.Microscopy.com 7, 26 -- Subject: Re: [Microscopy] SEM: Peltier Cooled Stage 7, 26 -- References: {200706111727.l5BHRWl0002146-at-ns.microscopy.com} 7, 26 -- In-Reply-To: {200706111727.l5BHRWl0002146-at-ns.microscopy.com} 7, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I use a Deben XP stage and am very happy with it. The XP does extended temperature at high end which if not needed, would suggest a different model.
Deben is in the UK.
gary g.
At 09:28 AM 6/11/2007, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Mon Jun 11 13:34:35 2007 10, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5BIYZYl025891 10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 13:34:35 -0500 10, 20 -- Message-Id: {200706111834.l5BIYZYl025891-at-ns.microscopy.com} 10, 20 -- Received: (qmail 14333 invoked from network); 11 Jun 2007 11:34:34 -0700 10, 20 -- Received: by simscan 1.1.0 ppid: 14330, pid: 14331, t: 0.0845s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp1 with SMTP; 11 Jun 2007 11:34:34 -0700 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Mon, 11 Jun 2007 11:33:52 -0800 10, 20 -- To: dale_batchelor-at-ncsu.edu 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] SEM: Peltier Cooled Stage 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200706111728.l5BHSSKB003892-at-ns.microscopy.com} 10, 20 -- References: {200706111728.l5BHSSKB003892-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-1841652B ==============================End of - Headers==============================
Voltage and current requirements vary depending upon the volume of aluminum you are evaporating. We coat substrates in the Ladd evaporator by starting out with a very low voltage and adjusting it higher till the aluminum starts to evaporate.
If you wish you can call Mike Bouchard at Ladd - 1-800-451-3406 to discuss particulars. He has many years of experience in the manufacture of the Ladd evaporator and substrate coating.
John Arnott
Disclaimer: Ladd sells electron microscopy supplies including a vacuum evaporator, coated grids and substrates.
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==============================Original Headers============================== 13, 27 -- From jd-at-laddresearch.com Mon Jun 11 16:07:06 2007 13, 27 -- Received: from mclaren.electric.net (mclaren.electric.net [216.129.90.240]) 13, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5BL7514014822 13, 27 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 16:07:05 -0500 13, 27 -- Received: from root by mclaren.electric.net with emc1-ok (Exim 4.62) 13, 27 -- (envelope-from {jd-at-laddresearch.com} ) 13, 27 -- id 1Hxr6N-0001kd-Ux; Mon, 11 Jun 2007 14:07:03 -0700 13, 27 -- Received: by emcmailer; Mon, 11 Jun 2007 14:07:03 -0700 13, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 13, 27 -- by mclaren.electric.net with esmtps (TLSv1:AES256-SHA:256) 13, 27 -- (Exim 4.62) 13, 27 -- (envelope-from {jd-at-laddresearch.com} ) 13, 27 -- id 1Hxr6M-0001gj-Tc; Mon, 11 Jun 2007 14:07:02 -0700 13, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 27 -- Date: Mon, 11 Jun 2007 17:06:21 -0400 13, 27 -- To: krensing-at-ucalgary.ca 13, 27 -- From: jd {jd-at-laddresearch.com} 13, 27 -- Subject: Re: [Microscopy] aluminum evaporation 13, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 13, 27 -- In-Reply-To: {200706111518.l5BFITO6019591-at-ns.microscopy.com} 13, 27 -- References: {200706111518.l5BFITO6019591-at-ns.microscopy.com} 13, 27 -- Mime-Version: 1.0 13, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 13, 27 -- X-Outbound-IP: 216.204.198.170 13, 27 -- X-Env-From: jd-at-laddresearch.com 13, 27 -- X-Virus-Status: Scanned by VirusSMART (c) 13, 27 -- Message-Id: {E1Hxr6N-0001kd-Ux-at-mclaren.electric.net} ==============================End of - Headers==============================
FEI Company has a current opening for a Sr. Applications Engineer with a background in front and back side circuit edit. This position is located in Hillsboro, Oregon and would be responsible for providing equipment testing, acceptance, demonstration, training, marketing, and sales support to current and potential customers. If you are interested, please follow the link to see the entire job description. Feel free to apply directly online and I will see every resume that comes in. We also have several other openings listed on our website as well.
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==============================Original Headers============================== 8, 27 -- From Joe.Williamson-at-fei.com Mon Jun 11 16:28:37 2007 8, 27 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5BLSajC031233 8, 27 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Jun 2007 16:28:36 -0500 8, 27 -- X-WSS-ID: 0JJHQC3-01-3DZ-01 8, 27 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 8, 27 -- by smtp.feico.com (Tumbleweed MailGate) with ESMTP id 604F5DA8A29 8, 27 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Jun 2007 14:28:50 -0700 (PDT) 8, 27 -- Received: from hlexc03.w2k.feico.com ([10.150.40.39]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.1830); 8, 27 -- Mon, 11 Jun 2007 14:28:35 -0700 8, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 27 -- Content-class: urn:content-classes:message 8, 27 -- MIME-Version: 1.0 8, 27 -- Content-Type: text/plain; 8, 27 -- charset="us-ascii" 8, 27 -- Subject: FW: FEI Job Opportunity Circuit Edit Applications Engineer 8, 27 -- Date: Mon, 11 Jun 2007 14:28:41 -0700 8, 27 -- Message-ID: {00948E5E66F1374FB9284A7B78138EB80BA7C294-at-hlexc03.w2k.feico.com} 8, 27 -- X-MS-Has-Attach: 8, 27 -- X-MS-TNEF-Correlator: 8, 27 -- Thread-Topic: FEI Job Opportunity Circuit Edit Applications Engineer 8, 27 -- Thread-Index: Acep/XONVuukpaH7Rnu5qqfoLnfa4AAAE8fgAJxlSXA= 8, 27 -- From: "Williamson, Joe" {Joe.Williamson-at-fei.com} 8, 27 -- To: {Microscopy-at-Microscopy.Com} 8, 27 -- X-OriginalArrivalTime: 11 Jun 2007 21:28:35.0665 (UTC) FILETIME=[77EBFC10:01C7AC6F] 8, 27 -- Content-Transfer-Encoding: 8bit 8, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5BLSajC031233 ==============================End of - Headers==============================
Dear colleagues We would like to bring to your attention the symposium on Nanoscale Phenomena in Functional Materials by Scanning Probe Microscopy, to be held at the Materials Research Society 2007 Fall meeting in Boston, MA. The symposium description is attached below and can also be accessed on the MRS web-site (http://www.mrs.org/s_mrs/sec.asp?CID=8005&DID=192095). The deadline for abstract submission is June 20. Looking forward to seeing you in Boston On behalf of the organizers Sergei V. Kalinin
Symposium B: Nanoscale Phenomena in Functional Materials by Scanning Probe Microscopy
The last decade has witnessed spectacular progress in the development and applications of scanning probe microscopy (SPM)-based nanoscale imaging techniques. The combination of high spatial resolution and sensitivity to local electronic, optical, and mechanical properties places these techniques among the most versatile tools for nanoscience, biology, physics, and materials science. Atomic and electronic structure of surfaces, vibrational excitations, energy flow, and local materials properties on the molecular level has become accessible with the advent of high-resolution SPMs. Electrostatic SPMs are being established as powerful techniques for spatially resolved studies of electronic transport on the nanometer level at electroactive interfaces and in molecular electronic devices such as carbon nanotubes. Dynamic SPM modes and scanning indentation techniques allow mechanical compliance and surface energy to be investigated at the nanoscale with applications to the emerging fields of nanotribology, nanofluidics, nanocomposites, and NEMS. Novel optical imaging modes, such as tip-enhanced spectroscopy, solid immersion microscopy, and apertureless scanning optical microscopy, have joined the now-established near-field scanning optical microscopy (NSOM), bringing the resolution of optical spectroscopy into the nanoscale regime and complementing local electronic measurements of materials with the STM family of instruments. These new measurement techniques were necessitated by the growing need for materials characterization on the nanoscale and have in turn led to the discovery of new nanoscale phenomena. Finally, the SPM has been used to manipulate and fabricate materials at the nanoscale.
It is the goal of this symposium to provide a multidisciplinary forum for scanning-probe-based materials and nanoscience in order to demonstrate the latest achievements in technique developments and materials applications that have led to scientific discoveries. The symposium will include two types of sessions: One will be dedicated to the recent advances in technique development of interest to the materials community and will bring together specialists in practical and theoretical aspects of SPM imaging. The second will focus on specific materials-related phenomena, including nanotubes and nanowires, quantum dots, surfaces, interfaces, and biological systems studied by local probe techniques.
The topics of the symposium will include, but not be limited to:
* Imaging, manipulation, and energy transfer on the atomic and molecular level by atomic resolution NC-AFM and STM * Local optical and electronic properties and excitations, e.g., plasmons measured with SPMs * Defects, impurities, dopants, and transport in semiconductor nanostructures, nanotubes, and nanowires * Mechanics and electromechanics on the nanoscale by SPM and nanoindentation * Mechanical and voltage nanolithography and surface modification * Energy flows and dissipation in materials, devices, and nanostructures * Transport in single-molecule devices and carbon nanotubes * Imaging and characterization of ferroelectric materials * Electronic properties of semiconductor heterostructures * Imaging and characterization of biological systems * Dynamics and imaging of polymers and soft materials
Invited speakers include: *Robert Carpick* (Univ. of Pennsylvania), *Levent Degertekin* (Georgia Inst. of Technology), *Dennis Discher* (Univ. of Pennsylvania), *Ricardo Garcia* (Univ. Madrid, Spain), *Franz Giessibl* (Univ. Augsburg, Germany), *Venkat Gopalan* (Pennsylvania State Univ.), *Jan Hoh* (Johns Hopkins Univ.), *Ernesto Joselevich* (Weizmann Inst. of Science, Israel), *Maki Kawai* (RIKEN, Japan), *L. Kuipers* (FOM, The Netherlands), *Alexander Malkin* (Lawrence Livermore National Lab), *Lukas Novotny* (Univ. of Rochester), *E. Ward* *Plummer* (Univ. of Tennessee/Oak Ridge National Lab), *V. Sandoghdar* (ETH Zurich, Switzerland), *M. Tomitori* (JAIST, Japan), and *S. Wilks* (Swansea Univ., United Kingdom).
*Symposium Organizers*
*Dawn** Bonnell* University of Pennsylvania, Nano/Bio Interface Center, 3231 Walnut St., Philadelphia, PA 19104 Tel 215-898-6231, Fax 215-746-3204, bonnell-at-lrsm.upenn.edu {mailto:bonnell-at-lrsm.upenn.edu}
*Sergei V. Kalinin* Oak Ridge National Laboratory, Materials Sciences and Technology Division and Center for Nanophase Materials Sciences, 1 Bethel Valley Rd., Oak Ridge, TN 37831 Tel 865-241-0236, Fax 865-574-4143, sergei2-at-ornl.gov {mailto:sergei2-at-ornl.gov}
*Sidney R. Cohen* Weizmann Institute of Science, Surface Analysis Laboratory, Rehovot 76100 Israel Tel 972-8-934-2703/3422, Fax 972-8-934-4137, sidney.cohen-at-weizmann.ac.il {mailto:sidney.cohen-at-weizmann.ac.il}
*Richard E. Palmer* University of Birmingham, School of Physics and Astronomy, Nanoscale Physics Research Laboratory, Birmingham B15 2TT, United Kingdom Tel 44-121-414-4653, Fax 44-121-414-7327, r.e.palmer-at-bham.ac.uk {mailto:r.e.palmer-at-bham.ac.uk}
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This Question was submitted to Ask-A-Microscopist by (L.Ryves-at-physics.usyd.edu.au) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, June 11, 2007 at 09:13:35 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both L.Ryves-at-physics.usyd.edu.au as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: L.Ryves-at-physics.usyd.edu.au Name: Luke Ryves
Organization: School of Physics / University of Sydney
Education: Graduate College
Location: Sydney, NSW, Australia
Title: Beam voltage choice to minimise charging
Question: Hi,
I'm just writing up my thesis and want to include some SEM images that were acquired for me ages ago. The samples were metal particles on SiO2 surfaces. At the time that we were taking the images I remember the microscopist explaining that the correct energy (2 to 5kV on a FEG SEM) can reduce charging, but I can't find a reference to this effect. Can you help me out by explaining how to choose the beam energy, and perhaps point me in the direction of some references?
It is a fundamental principle that lower KV reduces charging. Joy, Goldstein and Newbury, et. al. have written about this factor. In practice, we see it all the time.
The actual KV value depends on the specimen and the ability of the SEM to image what you want to see. I use 500V to 2KV on SiO2 and Hf oxides. This is useful up to about 60KX with Zeiss Supra 55VP in HV mode. Other systems will likely differ.
Hope this helps.
gary g.
At 04:26 PM 6/11/2007, you wrote:
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==============================Original Headers============================== 11, 21 -- From gary-at-gaugler.com Mon Jun 11 19:45:15 2007 11, 21 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5C0jFL6008744 11, 21 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 19:45:15 -0500 11, 21 -- Message-Id: {200706120045.l5C0jFL6008744-at-ns.microscopy.com} 11, 21 -- Received: (qmail 22413 invoked from network); 11 Jun 2007 17:45:15 -0700 11, 21 -- Received: by simscan 1.1.0 ppid: 22410, pid: 22411, t: 0.0990s 11, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 21 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 21 -- by qsmtp4 with SMTP; 11 Jun 2007 17:45:15 -0700 11, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 21 -- Date: Mon, 11 Jun 2007 17:45:08 -0800 11, 21 -- To: L.Ryves-at-physics.usyd.edu.au 11, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 21 -- Subject: Re: [Microscopy] AskAMicroscopist: Beam voltage choice to 11, 21 -- minimise charging 11, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 21 -- In-Reply-To: {200706120026.l5C0Qc1i032230-at-ns.microscopy.com} 11, 21 -- References: {200706120026.l5C0Qc1i032230-at-ns.microscopy.com} 11, 21 -- Mime-Version: 1.0 11, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-AC03E08 ==============================End of - Headers==============================
There is no set voltage/current to evaporate aluminium. A variable power source is used to achieve this. A current is gradually increased until aluminium melts and then a little bit more increase starts the evaporation.
We achieved good results using a tungsten wire with v-shaped bend on which a small v-shaped piece of Al wire was hung.
Alex
============== Alexander Titkov Senior Scientist
Millennium Inorganic Chemicals a Cristal Company Locked Bag 245 Bunbury WA 6230 AUSTRALIA
krensing-at-ucalgary .ca To: alex.titkov-at-millenniumchem.com cc: 11/06/2007 11:15 Subject: [Microscopy] aluminum evaporation PM Please respond to krensing
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Hello all, I have been asked what the voltage and current conditions are for evaporation of aluminum in a tungsten basket in a vacuum evaporator. Is this sort of information available in a reference? I don't have much experience with metal evaporation, so any advice would be appreciated. Thanks, Kim
-- Kim Rensing Ph.D. Assistant Research Professor Manager, Microscopy and Imaging Facility
University of Calgary Health Sciences Centre B129 - 3330 Hospital Drive NW Calgary, AB, Canada T2N 4N1 403-220-3488 krensing-at-ucalgary.ca
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==============================Original Headers============================== 34, 23 -- From alex.titkov-at-millenniumchem.com Mon Jun 11 19:53:38 2007 34, 23 -- Received: from edcpap01.lyondell.com (mail3.lyondell.com [161.16.0.77]) 34, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5C0rcCd020330 34, 23 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 19:53:38 -0500 34, 23 -- Received: from edcexp01.lyondell.com ([161.16.151.68]) 34, 23 -- by edcpap01.lyondell.com (8.13.7/8.13.7) with ESMTP id l5C0rbW5009602; 34, 23 -- Mon, 11 Jun 2007 19:53:37 -0500 34, 23 -- Received: from amnhhtv01.millenniumchem.com ([172.19.0.46]) by edcexp01.lyondell.com with Microsoft SMTPSVC(5.0.2195.6713); 34, 23 -- Mon, 11 Jun 2007 19:53:37 -0500 34, 23 -- Subject: Re: [Microscopy] aluminum evaporation 34, 23 -- To: krensing-at-ucalgary.ca 34, 23 -- Cc: microscopy-at-microscopy.com 34, 23 -- X-Mailer: Lotus Notes Release 5.0.11 July 24, 2002 34, 23 -- Message-ID: {OFB5F0477B.737A4C2C-ONC82572F8.0002DCD6-C82572F8.0004C359-at-millenniumchem.com} 34, 23 -- From: alex.titkov-at-millenniumchem.com 34, 23 -- Date: Tue, 12 Jun 2007 09:51:51 +0900 34, 23 -- X-MIMETrack: Serialize by Router on AMNHHTV01/HUB/SRV/MIC(Release 5.0.11 |July 24, 2002) at 34, 23 -- 06/11/2007 08:53:37 PM 34, 23 -- MIME-Version: 1.0 34, 23 -- Content-type: text/plain; charset=us-ascii 34, 23 -- X-OriginalArrivalTime: 12 Jun 2007 00:53:37.0617 (UTC) FILETIME=[1C74E410:01C7AC8C] 34, 23 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=3.1.0-0705030000 definitions=main-0706110097 34, 23 -- X-Proofpoint-OriginalSender: alex.titkov-at-millenniumchem.com ==============================End of - Headers==============================
You can find information about varying the beam voltage in most any edition of "Scanning Electron Microscopy and X-Ray Microanalysis" (usually referred to as "SEMXM") by Goldstein, Newbury, Echlin, Joy, Fiori, and Lifshin. Look for information on the production efficiency of Secondary Electrons as a function of beam voltage. If I remember correctly, the SE coefficient went above 1 roughly between 800V and 1.5kV for SiO2 (anyone want to correct me?).
Cheers, Henk
At 08:26 PM 6/11/2007, you wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility (614) 292-0674 040 Fontana Labs, 116 W. 19th Ave www.ceof.ohio-state.edu
==============================Original Headers============================== 9, 27 -- From colijn.1-at-osu.edu Mon Jun 11 20:41:01 2007 9, 27 -- Received: from ER6S1.ENG.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5C1f1OS000377 9, 27 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 20:41:01 -0500 9, 27 -- Received: from CONVERSION-DAEMON.er6s1.eng.ohio-state.edu by 9, 27 -- er6s1.eng.ohio-state.edu (PMDF V6.2-1x11 #31056) 9, 27 -- id {01MHOEESUQ2OAAO8BG-at-er6s1.eng.ohio-state.edu} for 9, 27 -- microscopy-at-microscopy.com; Mon, 11 Jun 2007 21:41:00 -0400 (EDT) 9, 27 -- Received: from HOC3.osu.edu 9, 27 -- (d149-67-33-174.col.wideopenwest.com [67.149.174.33]) 9, 27 -- by er6s1.eng.ohio-state.edu (PMDF V6.2-1x11 #31056) 9, 27 -- with ESMTPA id {01MHOEES1H3GA9S4CM-at-er6s1.eng.ohio-state.edu} ; Mon, 9, 27 -- 11 Jun 2007 21:41:00 -0400 (EDT) 9, 27 -- Date: Mon, 11 Jun 2007 21:40:34 -0400 9, 27 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 9, 27 -- Subject: Re: [Microscopy] AskAMicroscopist: Beam voltage choice to minimise 9, 27 -- charging 9, 27 -- In-reply-to: {200706120026.l5C0QWb1032015-at-ns.microscopy.com} 9, 27 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 9, 27 -- To: L.Ryves-at-physics.usyd.edu.au 9, 27 -- Cc: microscopy-at-microscopy.com 9, 27 -- Message-id: {7.0.1.0.2.20070611212812.010d11f8-at-osu.edu} 9, 27 -- MIME-version: 1.0 9, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 9, 27 -- Content-type: text/plain; charset=us-ascii; format=flowed 9, 27 -- X-Env-From: auth/colijn.1-at-osu.edu 9, 27 -- References: {200706120026.l5C0QWb1032015-at-ns.microscopy.com} ==============================End of - Headers==============================
That is quite probably true. That is why I use 1KV and then 2KV. Beyond these values, specimens charge too much to be of use....IMO.
Other ILDs besides SiO2 are quite interesting and challenging. Of course, the gate oxides are another challenge.
gary g.
At 05:42 PM 6/11/2007, you wrote:
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First, you want to use a fast scan and average the frames. Just use enough frames in the average so that the updated image doesn't take too long. This is a dynamic affect and helps a lot. Slow scans are more difficult to set up the low voltage imaging. I can tell you that from personal experience on an older non-digital Hitachi S-900. When you went to slow scan to shoot the picture on Polaroid after using TV rates to set up a good charge balance image, you got charging in the recorded image.
Secondly, you need to find the correct voltage for charge balance. This is where the number of electrons (BSE and SE) is the same as incident number of electrons, i.e. beam current. For most insulators, as mentioned before, the voltage will be somewhere between 1 and 2 kV for a flat sample at zero tilt. If I remember correctly, go to a mag of about 1000 X, stay there for a little bit, then up the mag to 5000 X and stay there for about 20-30 seconds, then return quickly back to 1000 X. If you don't have charge balance, you will see a box in the lower mag image. Look quickly, because it will change. If the contrast of the box is bright relative to the low mag area, then your box had charged negatively and you are above the balance accelerating voltage. If the box was dark, then you were below the voltage and you have to increase the voltage. If it doesn't change contrast, you were JUST right. Eat your porridge and go take some nice pictures after your nap.
Also note, that for tilted samples the voltage value will increase. For example, on the JEOL Auger system, you could take uncoated insulators and do analysis at a beam voltage of 3 kV if the sample was tilted to an angle of about 70 degrees. This is because of the increase in both BSE and SE signals at the higher tilt angles. Essentially, the voltage shifted from about 1 kV at zero tilt to 3 kV at 70 degrees.
Hope this helps.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
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This is summarized in Goldstein's section 4.8.2., which is titled Charging.
Scanning Electron Microscopy and X-Ray Microanalysis, A Text for Biologists, Materials Scientists, and Geologists, 2nd Ed., Goldstein, J.I., et al., 1992.
-- Bernard R. Cuzzillo, Ph.D., P.E. President, Mechanical Engineer, and Fire Scientist Berkeley Research Company (BRC) 600 Addison Street Berkeley, CA 94710-1920 USA
bernard-at-berkeleyrc.com
==============================Original Headers============================== 4, 28 -- From biggerdadda-at-gmail.com Tue Jun 12 01:07:07 2007 4, 28 -- Received: from an-out-0708.google.com (an-out-0708.google.com [209.85.132.246]) 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5C676GN006249 4, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Jun 2007 01:07:07 -0500 4, 28 -- Received: by an-out-0708.google.com with SMTP id b33so412916ana 4, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Jun 2007 23:07:06 -0700 (PDT) 4, 28 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 4, 28 -- d=gmail.com; s=beta; 4, 28 -- h=domainkey-signature:received:received:message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 4, 28 -- b=WvRiehw+6OLNtmCLwELIiTp3iavvD0+LbWuvAOouhbpW7ONHYh+fVM7oQpyF5BxevGr1TFNL0bQS8tiTMPzRjHVTM/hNXBthFjxLgyLg3ol/VAyeIGpji81w/L6NGCsj4mJQaZNVSa/6v1L5sFQLhgf+gcidKjZpI016Ar6h18c= 4, 28 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 28 -- d=gmail.com; s=beta; 4, 28 -- h=received:message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 4, 28 -- b=hIjgZA7Fbr+Wzz+jUYROy95/KJAyPDyEOdhXaAJrQV7r9XUgMTY9b2+ddLr8uku8hd2+m1KOn6YjRdWFYYraZWo+qFxTCDqDCK5O0Zk3zOHb0VimdQYsIbPzKA9hUu3gpTliLZj/ehjIVuG1jaU9aCIEY5/bcuaukaW/wxY/POQ= 4, 28 -- Received: by 10.100.153.17 with SMTP id a17mr3799768ane.1181628426573; 4, 28 -- Mon, 11 Jun 2007 23:07:06 -0700 (PDT) 4, 28 -- Received: by 10.100.13.19 with HTTP; Mon, 11 Jun 2007 23:07:06 -0700 (PDT) 4, 28 -- Message-ID: {ef580e610706112307n35bde340na917f752fcc69d87-at-mail.gmail.com} 4, 28 -- Date: Mon, 11 Jun 2007 23:07:06 -0700 4, 28 -- From: "Bernard R. Cuzzillo, Ph.D., P.E." {bernard-at-berkeleyrc.com} 4, 28 -- Sender: biggerdadda-at-gmail.com 4, 28 -- To: Microscopy-at-microscopy.com 4, 28 -- Subject: Re: Specimen charging reference 4, 28 -- MIME-Version: 1.0 4, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 28 -- Content-Transfer-Encoding: 7bit 4, 28 -- Content-Disposition: inline 4, 28 -- X-Google-Sender-Auth: cb49c611c4954e3f ==============================End of - Headers==============================
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This is summarized in Goldstein's section 4.8.2., } which is titled Charging. } } Scanning Electron Microscopy and X-Ray } Microanalysis, A Text for } Biologists, Materials Scientists, and Geologists, } 2nd Ed., Goldstein, } J.I., et al., 1992. } } -- } Bernard R. Cuzzillo, Ph.D., P.E. } President, Mechanical Engineer, and Fire Scientist } Berkeley Research Company (BRC) } 600 Addison Street } Berkeley, CA 94710-1920 } USA } } bernard-at-berkeleyrc.com } } ==============================Original } Headers============================== } 4, 28 -- From biggerdadda-at-gmail.com Tue Jun 12 } 01:07:07 2007 } 4, 28 -- Received: from an-out-0708.google.com } (an-out-0708.google.com [209.85.132.246]) } 4, 28 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l5C676GN006249 } 4, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 12 } Jun 2007 01:07:07 -0500 } 4, 28 -- Received: by an-out-0708.google.com with
A number of people have already explained the basic ideas and given you relevant references. I just wish to add that you can find experimental curves of secondary yield vs. acceleration voltage (including curves for SiO2) at the webaddress
http://www.mc-set.com/bse/
Best regards,
Jørgen B. Bilde-Sørensen senior scientist, ph.d. Phone direct +45 4677 5802 j.bilde-at-risoe.dk
Materials Research Department Risø National Laboratory Technical University of Denmark - DTU Building 228, P.O. Box 49 DK-4000 Roskilde, Denmark Tel +45 4677 5700 Fax +45 4677 5758 www.risoe.dk
X-from 1 January 2007, Risø National Laboratory, the Danish Institute for Food and Veterinary Research, the Danish Institute for Fisheries Research, the Danish National Space Center and the Danish Transport Research Institute have been merged with the Technical University of Denmark (DTU) with DTU as the continuing unit.
-----Original Message----- X-from: L.Ryves-at-physics.usyd.edu.au [mailto:L.Ryves-at-physics.usyd.edu.au] Sent: Tuesday, June 12, 2007 2:30 AM To: j.bilde-at-risoe.dk
This Question was submitted to Ask-A-Microscopist by (L.Ryves-at-physics.usyd.edu.au) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, June 11, 2007 at 09:13:35 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both L.Ryves-at-physics.usyd.edu.au as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: L.Ryves-at-physics.usyd.edu.au Name: Luke Ryves
Organization: School of Physics / University of Sydney
Education: Graduate College
Location: Sydney, NSW, Australia
Title: Beam voltage choice to minimise charging
Question: Hi,
I'm just writing up my thesis and want to include some SEM images that were acquired for me ages ago. The samples were metal particles on SiO2 surfaces. At the time that we were taking the images I remember the microscopist explaining that the correct energy (2 to 5kV on a FEG SEM) can reduce charging, but I can't find a reference to this effect. Can you help me out by explaining how to choose the beam energy, and perhaps point me in the direction of some references?
Thanks to everybody who replied to my query about imaging carbon nanoparticles in tissue. I will prepare a summary of the replies soon and post it for the list.
As usual, this list has been a great resource. Thanks again.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 5, 23 -- From TindallR-at-missouri.edu Tue Jun 12 08:43:12 2007 5, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5CDhBU0017561 5, 23 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jun 2007 08:43:12 -0500 5, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 5, 23 -- Tue, 12 Jun 2007 08:43:11 -0500 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 23 -- Content-class: urn:content-classes:message 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="us-ascii" 5, 23 -- Subject: Carbon nanos in tissue 5, 23 -- Date: Tue, 12 Jun 2007 08:43:11 -0500 5, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BAFF-at-UM-XMAIL08.um.umsystem.edu} 5, 23 -- X-MS-Has-Attach: 5, 23 -- X-MS-TNEF-Correlator: 5, 23 -- Thread-Topic: Carbon nanos in tissue 5, 23 -- Thread-Index: Aces954zmMRlK74HSEm2B+CV51la1g== 5, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- X-OriginalArrivalTime: 12 Jun 2007 13:43:11.0342 (UTC) FILETIME=[9E2430E0:01C7ACF7] 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5CDhBU0017561 ==============================End of - Headers==============================
Stephanie: the 3rd edition is the most recent, but I think the 2nd edition is more useful to the novice/intermediate user. The section cited in the message by Dr. Cuzzillo is for the 2nd edition and is not in the 3rd edition as its own section. The information may be scattered around in Section 4 but I haven't sat down and looked for it.
Just an FYI: if you buy the 3rd edition as a used book, make sure it has the CD with it.
nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } After an internet search I found this book. Is this } the last edition of the same book? } } Scanning Electron Microscopy and X-ray Microanalysis } Goldstein, J., Newbury, D.E., Joy, D.C., Lyman, C.E., } Echlin, P., Lifshin, E., Sawyer, L.C., Michael, J.R. } } 3rd ed. 2003. Corr. 4th printing, 2007, 586 p., with 5 } pg 4/C insert, Hardcover } } Best regards, } } Stephane } } --- bernard-at-berkeleyrc.com wrote: } } ---------------------------------------------------------------------------- } } This is summarized in Goldstein's section 4.8.2., } } which is titled Charging. } } } } Scanning Electron Microscopy and X-Ray } } Microanalysis, A Text for } } Biologists, Materials Scientists, and Geologists, } } 2nd Ed., Goldstein, } } J.I., et al., 1992. } } } } -- } } Bernard R. Cuzzillo, Ph.D., P.E. } } President, Mechanical Engineer, and Fire Scientist } } Berkeley Research Company (BRC) } } 600 Addison Street } } Berkeley, CA 94710-1920 } } USA } } } } bernard-at-berkeleyrc.com } } } } ==============================Original } } Headers============================== } } 4, 28 -- From biggerdadda-at-gmail.com Tue Jun 12 } } 01:07:07 2007 } } 4, 28 -- Received: from an-out-0708.google.com } } (an-out-0708.google.com [209.85.132.246]) } } 4, 28 -- by ns.microscopy.com } } (8.12.11.20060308/8.12.8) with ESMTP id } } l5C676GN006249 } } 4, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 12 } } Jun 2007 01:07:07 -0500 } } 4, 28 -- Received: by an-out-0708.google.com with
Is there a website for attendees looking to share hotel rooms for the MandM meeting in Ft. Lauderdale?
thanks, Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
************************************************************************ ****** The box said "Requires WindowsXP or better", so I bought a Macintosh. ************************************************************************ ******
==============================Original Headers============================== 7, 24 -- From glenmac-at-u.washington.edu Wed Jun 13 12:50:47 2007 7, 24 -- Received: from mxout3.cac.washington.edu (mxout3.cac.washington.edu [140.142.32.166]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5DHolF1014877 7, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jun 2007 12:50:47 -0500 7, 24 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.33.9] (may be forged)) 7, 24 -- by mxout3.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.05) with ESMTP id l5DHoktD002647 7, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 7, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jun 2007 10:50:46 -0700 7, 24 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) 7, 24 -- (authenticated authid=glenmac) 7, 24 -- by smtp.washington.edu (8.13.7+UW06.06/8.13.7+UW07.03) with ESMTP id l5DHokkR006833 7, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 7, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jun 2007 10:50:46 -0700 7, 24 -- Mime-Version: 1.0 (Apple Message framework v752.2) 7, 24 -- Content-Transfer-Encoding: 7bit 7, 24 -- Message-Id: {987A552F-56B8-42F4-8EC5-5CEFBBC9729A-at-u.washington.edu} 7, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 24 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {Microscopy-at-microscopy.com} 7, 24 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 7, 24 -- Subject: MandM roommate site 7, 24 -- Date: Wed, 13 Jun 2007 10:50:44 -0700 7, 24 -- X-Mailer: Apple Mail (2.752.2) 7, 24 -- X-PMX-Version: 5.3.1.294258, Antispam-Engine: 2.5.1.298604, Antispam-Data: 2007.6.13.102633 7, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
Sorry Glen, but there is no site like this setup by the Meeting.
Nestor Your Friendly Neighborhood SysOp
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-- =========================================== Dr. Nestor J. Zaluzec Argonne National Lab Electron Microscopy Center Materials Science Division/Bldg 212 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov =========================================== TPMLab: http://tpm.amc.anl.gov MMSite: http://www.amc.anl.gov ===========================================
The box said ... "This program requires Win 95/98/NT or better..." So I bought a Mac !
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==============================Original Headers============================== 11, 16 -- From zaluzec-at-aaem.amc.anl.gov Wed Jun 13 13:04:39 2007 11, 16 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3]) 11, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5DI4d0a026455 11, 16 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jun 2007 13:04:39 -0500 11, 16 -- Received: from [146.139.72.105] (aem105.amc.anl.gov [146.139.72.105]) 11, 16 -- by aaem.amc.anl.gov (8.12.11.20060308/8.12.10) with ESMTP id l5DI4cja008110 11, 16 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jun 2007 13:04:39 -0500 11, 16 -- Mime-Version: 1.0 11, 16 -- Message-Id: {p0624080ec295e0ed3803-at-[146.139.72.105]} 11, 16 -- In-Reply-To: {200706131750.l5DHolXb014884-at-ns.microscopy.com} 11, 16 -- References: {200706131750.l5DHolXb014884-at-ns.microscopy.com} 11, 16 -- Date: Wed, 13 Jun 2007 13:04:36 -0500 11, 16 -- To: microscopy-at-microscopy.com 11, 16 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} 11, 16 -- Subject: Re: This is no MandM roommate site 11, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I have recently been having problems with my field emission scope lately at magnifications above 50KX. The image seems to swim and sparkles are seen and the focus boundaries. A slow scan image impossible to acquire. The ground is suspected. It is currently grounded to the building at the same location as all the rest of the analytical equipment. Our electricians are in the process of rerunning a separate ground for the scope. They are going to put an eight foot copper rod in the earth outside of my lab drill hole in the wall for wires to go through and attach it to the ground wires to the back of the scope. I was wondering how the rest of have run the grounds for your scopes. Any advice would be appreciated.
Lori Ables Solutia, Inc. laable-at-solutia.com
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==============================Original Headers============================== 6, 33 -- From laable-at-solutia.com Thu Jun 14 08:50:38 2007 6, 33 -- Received: from plmler9.mail.eds.com (plmler9.mail.eds.com [199.228.142.117]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EDoctm031340 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:38 -0500 6, 33 -- Received: from plmlir3.mail.eds.com (plmlir3-2.mail.eds.com [199.228.142.133]) 6, 33 -- by plmler9.mail.eds.com (8.13.8/8.13.8) with ESMTP id l5EDoYCV022081 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:36 -0500 6, 33 -- Received: from plmlir3.mail.eds.com (localhost.localdomain [127.0.0.1]) 6, 33 -- by plmlir3.mail.eds.com (8.13.8/8.12.10) with ESMTP id l5EDoCXS028914 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:12 -0500 6, 33 -- Received: from mailgw2.solutia.com ([205.191.166.96]) 6, 33 -- by plmlir3.mail.eds.com (8.13.8/8.12.10) with ESMTP id l5EDoBND028887 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:12 -0500 6, 33 -- Received: from USAHMSLSOIEX2.soi.dir.solutia.com ([205.191.166.99]) by mailgw2.solutia.com with Microsoft SMTPSVC(6.0.3790.1830); Thu, 14 Jun 2007 09:50:11 -0400 6, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.2826 6, 33 -- Content-Class: urn:content-classes:message 6, 33 -- MIME-Version: 1.0 6, 33 -- Content-Type: text/plain; 6, 33 -- charset="us-ascii" 6, 33 -- Subject: SEM Ground 6, 33 -- Date: Thu, 14 Jun 2007 09:50:08 -0400 6, 33 -- Message-ID: {7B97BC1F2986504EABA8A0B1C780A5C301E77C67-at-USAHMSLSOIEX2.soi.dir.solutia.com} 6, 33 -- Importance: normal 6, 33 -- Priority: normal 6, 33 -- X-MS-Has-Attach: 6, 33 -- X-MS-TNEF-Correlator: 6, 33 -- Thread-Topic: SEM Ground 6, 33 -- thread-index: AceuiupcKKVbl+U3T3uHsV9HIQBKdA== 6, 33 -- From: "Ables, Lori A" {laable-at-solutia.com} 6, 33 -- To: {Microscopy-at-Microscopy.Com} 6, 33 -- X-OriginalArrivalTime: 14 Jun 2007 13:50:11.0422 (UTC) FILETIME=[ED5ACFE0:01C7AE8A] 6, 33 -- Content-Transfer-Encoding: 8bit 6, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5EDoctm031340 ==============================End of - Headers==============================
You have not described the operating conditions or the samples. Has anything changed in those regards? Perhaps the matter is one of sample charging more so that instrumentation problems. I would certainly address those issues first with an application specialist or the list before resorting to the wiring change. That is not likely to be cheap.
If it is the ground, it sounds like the remedy is reasonable. It sounds like your people are on the right path.
Warren
________________________________________ X-from: laable-at-solutia.com [mailto:laable-at-solutia.com] Sent: Thu 6/14/2007 8:51 AM To: wesaia-at-iastate.edu
To All Listers,
I have recently been having problems with my field emission scope lately at magnifications above 50KX. The image seems to swim and sparkles are seen and the focus boundaries. A slow scan image impossible to acquire. The ground is suspected. It is currently grounded to the building at the same location as all the rest of the analytical equipment. Our electricians are in the process of rerunning a separate ground for the scope. They are going to put an eight foot copper rod in the earth outside of my lab drill hole in the wall for wires to go through and attach it to the ground wires to the back of the scope. I was wondering how the rest of have run the grounds for your scopes. Any advice would be appreciated.
Lori Ables Solutia, Inc. laable-at-solutia.com
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==============================Original Headers============================== 11, 31 -- From wesaia-at-iastate.edu Thu Jun 14 10:37:07 2007 11, 31 -- Received: from mailhub-3.iastate.edu (mailhub-3.iastate.edu [129.186.140.13]) 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EFb7CG013672 11, 31 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 14 Jun 2007 10:37:07 -0500 11, 31 -- Received: from mailout-2.iastate.edu (mailout-2.iastate.edu [129.186.140.2]) 11, 31 -- by mailhub-3.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id l5EFb6XL005530; 11, 31 -- Thu, 14 Jun 2007 10:37:06 -0500 11, 31 -- Received: from (owa.eng.iastate.edu [129.186.23.85]) by mailout-2.iastate.edu with smtp 11, 31 -- id 4744_9a3b25d0_1a8c_11dc_996b_001372578af6; 11, 31 -- Thu, 14 Jun 2007 10:33:29 -0500 11, 31 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 11, 31 -- Thu, 14 Jun 2007 10:37:07 -0500 11, 31 -- Content-class: urn:content-classes:message 11, 31 -- MIME-Version: 1.0 11, 31 -- Content-Type: text/plain; 11, 31 -- charset="iso-8859-1" 11, 31 -- Subject: RE: [Microscopy] SEM Ground 11, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 31 -- Date: Thu, 14 Jun 2007 10:37:41 -0500 11, 31 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72701A9EDFC-at-maire.eng.iastate.edu} 11, 31 -- X-MS-Has-Attach: 11, 31 -- X-MS-TNEF-Correlator: 11, 31 -- Thread-Topic: [Microscopy] SEM Ground 11, 31 -- Thread-Index: AceuiyhSKgw8zd0uSyisUNdBIUpgkAAAIZZ/ 11, 31 -- References: {200706141351.l5EDpmKh032623-at-ns.microscopy.com} 11, 31 -- From: "Straszheim, Warren E [CCE E]" {wesaia-at-iastate.edu} 11, 31 -- To: "MSA" {Microscopy-at-msa.microscopy.com} 11, 31 -- Cc: {laable-at-solutia.com} 11, 31 -- X-OriginalArrivalTime: 14 Jun 2007 15:37:07.0035 (UTC) FILETIME=[DD5BAAB0:01C7AE99] 11, 31 -- Content-Transfer-Encoding: 8bit 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5EFb7CG013672 ==============================End of - Headers==============================
Greetings all--I am seeking input on what appears to be Carbon contamination. Here is the situation.
Take a Pella 16111-9 stub out of bag and put on holder. Take another stub out of bag and sputter coat with Pd and put on holder. Do EDS on both.
un-coated: wt% at% C 7.5 15 O 4.5 7 Al 88 78
coated: C 23 38 O 6.5 8 Al 71 53
SEM is Zeiss Supra 55VP with Edwards XDS10 dry scroll pump and turbo. Coater is Denton Desk IV with Edwards XDS5 and turbo.
The goal of using non-oil pumps was to reduce hydrocarbon contamination. So, where is the C coming from? Nothing has been done to the scroll pumps since new. There are kits for repairing them but when is this necessary and what would indicate that it be done? Would high C be an indicator?
I'm stumped on this one.
Any ideas?
gary g.
==============================Original Headers============================== 10, 17 -- From gary-at-gaugler.com Thu Jun 14 11:54:47 2007 10, 17 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5EGslUC027192 10, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 11:54:47 -0500 10, 17 -- Message-Id: {200706141654.l5EGslUC027192-at-ns.microscopy.com} 10, 17 -- Received: (qmail 15711 invoked from network); 14 Jun 2007 09:54:47 -0700 10, 17 -- Received: by simscan 1.1.0 ppid: 15708, pid: 15709, t: 0.1452s 10, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 17 -- by qsmtp4 with SMTP; 14 Jun 2007 09:54:46 -0700 10, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 17 -- Date: Thu, 14 Jun 2007 09:54:46 -0800 10, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 10, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 17 -- Subject: Carbon "contamination" 10, 17 -- Mime-Version: 1.0 10, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-20B34597 ==============================End of - Headers==============================
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Can't help with the uncoated stub, but most of the "C Ka" you are seeing is presumably from the Pd Mz line which is at 43.36 A (vs the nominal 44.0 A for C Ka).
John -- ======================================================== John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480 Cameron Electron Microprobe Lab lab: (608) 265-4798 Dept of Geology & Geophysics fax: (608) 262-0693 University of Wisconsin home: (608) 274-2245 1215 West Dayton St. email: johnf-at-geology.wisc.edu Madison, WI 53706 amateur radio: WA3BTA Personal http://www.geology.wisc.edu/~johnf/ Probe lab http://www.geology.wisc.edu/~johnf/sx51.html Probe Sign Up Calender: http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi
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==============================Original Headers============================== 5, 25 -- From johnf-at-geology.wisc.edu Thu Jun 14 12:30:30 2007 5, 25 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EHUUQO007298 5, 25 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 12:30:30 -0500 5, 25 -- Received: from localhost (localhost [127.0.0.1]) 5, 25 -- by localhost (Postfix) with ESMTP id 3ED2020D06; 5, 25 -- Thu, 14 Jun 2007 12:30:30 -0500 (CDT) 5, 25 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) 5, 25 -- by localhost (geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 5, 25 -- with ESMTP id 13024-01; Thu, 14 Jun 2007 12:30:17 -0500 (CDT) 5, 25 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57]) 5, 25 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 5, 25 -- (No client certificate requested) 5, 25 -- by ice.geology.wisc.edu (Postfix) with ESMTP id 79EBB20D19; 5, 25 -- Thu, 14 Jun 2007 12:30:17 -0500 (CDT) 5, 25 -- Mime-Version: 1.0 5, 25 -- Message-Id: {p06230903c2972a7aaaed-at-[144.92.206.57]} 5, 25 -- In-Reply-To: {200706141659.l5EGxk54002427-at-ns.microscopy.com} 5, 25 -- References: {200706141659.l5EGxk54002427-at-ns.microscopy.com} 5, 25 -- Date: Thu, 14 Jun 2007 12:30:13 -0500 5, 25 -- To: gary-at-gaugler.com, microscopy-at-microscopy.com 5, 25 -- From: John Fournelle {johnf-at-geology.wisc.edu} 5, 25 -- Subject: Re: [Microscopy] Carbon "contamination" 5, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 25 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu ==============================End of - Headers==============================
One can buy a 40-100x oil objective with an iris diaphram. What is the theoretical difference in performance between imaging with a low NA dry objective (.6NA for example) and an equivalent magnification, high NA oil objective with the iris partially closed to reduce the NA to .6? Should they be similar? Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 5, 19 -- From david.knecht-at-uconn.edu Thu Jun 14 12:40:46 2007 5, 19 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EHekrM018923 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 12:40:46 -0500 5, 19 -- Received: from [137.99.46.174] (d46h174.public.uconn.edu [137.99.46.174]) 5, 19 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id l5EHeffX009795 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 13:40:41 -0400 5, 19 -- Mime-Version: 1.0 (Apple Message framework v752.3) 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- Message-Id: {D06FF75E-9614-4D26-BA77-9425A50AFCAD-at-uconn.edu} 5, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 19 -- To: microscopy {microscopy-at-microscopy.com} 5, 19 -- From: David Knecht {david.knecht-at-uconn.edu} 5, 19 -- Subject: objective NA 5, 19 -- Date: Thu, 14 Jun 2007 13:40:34 -0400 5, 19 -- X-Mailer: Apple Mail (2.752.3) 5, 19 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information. 5, 19 -- X-UConn-MailScanner: Found to be clean 5, 19 -- X-UConn-MailScanner-SpamCheck: ==============================End of - Headers==============================
The electricians have finished my ground and the result is amazing. I can now obtain a decent slow scan image at 500KX. Thanks to all who replied.
Lori
-----Original Message----- X-from: Ables, Lori A Sent: Thursday, June 14, 2007 8:50 AM To: ' (Microscopy-at-Microscopy.Com)'
Gary,
You're pretty clever, Gary, and I'm sure you've already done this. But......have you checked for any exposed wiring, gaskets or seals near the target that might be degraded when the plasma is activated? Are you using Argon gas to vent the system and as the source of plasma? If so, how clean is the gas used to generate plasma? Sometimes, gas cylinders contain traces of oil (as might the pressure regulators). Check the threads for the presence of a lubricant. Anyone else using the system? If so, they may have left some contamination inside the chamber (like finger oils).
If you are still having problems, try putting a coated TEM grid inside the chamber and examine it for traces of contamination. Sometimes, "seeing" the contamination is a clue to where it may be coming from.
Good luck.......
John B.
} Greetings all--I am seeking input on what appears } to be Carbon contamination. Here is the situation. } } Take a Pella 16111-9 stub out of bag and put on } holder. Take another stub out of bag and sputter } coat with Pd and put on holder. Do EDS on both. } The goal of using non-oil pumps was to reduce hydrocarbon } contamination. So, where is the C coming from? Nothing } has been done to the scroll pumps since new. There are } kits for repairing them but when is this necessary and } what would indicate that it be done? Would high C } be an indicator? }
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Lori, I am pleased that you saw such an improvement. Your original ground connection must have been pretty bad!
Because of the importance of an electrically quiet and isolated instrument ground, most instrument manufacturers won't install the instrument unless one is available. For example FEI quotes a less than 0.1 ohm resistance requirement and that doesn't even address the electrical noise issue.
In the new integrated science complex (underground shared instrumentation facilities) building that we are putting the finishing touches on here at Univ of Oregon, we designed in separate isolated, instrument grounds for each instrument. We had to negotiate with the electrical inspector to get this through and it wasn't easy. john
At 10:49 AM 6/14/2007, you wrote:
} All, } } The electricians have finished my ground and the result is } amazing. I can now obtain a decent slow scan image at 500KX. Thanks } to all who replied. } } Lori } } -----Original Message----- } X-from: Ables, Lori A } Sent: Thursday, June 14, 2007 8:50 AM } To: ' (Microscopy-at-Microscopy.Com)' } Subject: SEM Ground } } To All Listers, } } I have recently been having problems with my field emission scope } lately at magnifications above 50KX. The image seems to swim and } sparkles are seen and the focus boundaries. A slow scan image } impossible to acquire. The ground is suspected. It is currently } grounded to the building at the same location as all the rest of the } analytical equipment. Our electricians are in the process of } rerunning a separate ground for the scope. They are going to put an } eight foot copper rod in the earth outside of my lab drill hole in } the wall for wires to go through and attach it to the ground wires } to the back of the scope. I was wondering how the rest of have run } the grounds for your scopes. Any advice would be appreciated. } } Lori Ables } Solutia, Inc. } laable-at-solutia.com } } } This electronic mail message is intended exclusively for the } individual or entity to which it is addressed. } This message, together with any attachment, may contain Solutia } confidential and privileged information. } The recipient is hereby put on notice to treat the information as } confidential and privileged and to not disclose or use the } information except as authorized by Solutia. } Any unauthorized review, printing, retention, copying, disclosure, } distribution, retransmission, dissemination or other use of, or } taking of any action in reliance upon, this information by persons } or entities other than the intended recipient is prohibited. If you } received this message in error, please immediately contact the } sender by reply email and delete all copies of the material from any } computer. Thank you for your cooperation.
==============================Original Headers============================== 7, 21 -- From donovan-at-uoregon.edu Thu Jun 14 13:23:37 2007 7, 21 -- Received: from smtp.uoregon.edu (mserv4.uoregon.edu [128.223.142.54]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EINai4021959 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 13:23:37 -0500 7, 21 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.92.75]) 7, 21 -- (authenticated bits=0) 7, 21 -- by smtp.uoregon.edu (8.13.8/8.13.8) with ESMTP id l5EINaWW007377 7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 11:23:36 -0700 7, 21 -- Message-Id: {200706141823.l5EINaWW007377-at-smtp.uoregon.edu} 7, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 21 -- Date: Thu, 14 Jun 2007 11:22:31 -0700 7, 21 -- To: microscopy-at-microscopy.com 7, 21 -- From: John Donovan {donovan-at-uoregon.edu} 7, 21 -- Subject: Re: [Microscopy] FW: SEM Ground 7, 21 -- In-Reply-To: {200706141749.l5EHn4tW006932-at-ns.microscopy.com} 7, 21 -- References: {200706141749.l5EHn4tW006932-at-ns.microscopy.com} 7, 21 -- Mime-Version: 1.0 7, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 21 -- X-Virus-Scanned: ClamAV 0.90.3/3419/Thu Jun 14 06:49:39 2007 on mserv4 7, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Using basic theory the resolution should be the same [d = (Wavelength)/(2*NA)]. The fourier plane would be slightly different but not sure of the effects.
The depth of field should be (theoretically) better with an oil/water immersion stopped down. I haven't tested this personally.
Practically, I've noticed that there is less noise (scattered) light with both my oil and water immersion objectives.
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
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-----Original Message----- X-from: david.knecht-at-uconn.edu [mailto:david.knecht-at-uconn.edu] Sent: Thursday, June 14, 2007 1:45 PM To: ph2-at-sprynet.com
One can buy a 40-100x oil objective with an iris diaphram. What is the theoretical difference in performance between imaging with a low NA dry objective (.6NA for example) and an equivalent magnification, high NA oil objective with the iris partially closed to reduce the NA to .6? Should they be similar? Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 5, 19 -- From david.knecht-at-uconn.edu Thu Jun 14 12:40:46 2007 5, 19 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EHekrM018923 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 12:40:46 -0500 5, 19 -- Received: from [137.99.46.174] (d46h174.public.uconn.edu [137.99.46.174]) 5, 19 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id l5EHeffX009795 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 13:40:41 -0400 5, 19 -- Mime-Version: 1.0 (Apple Message framework v752.3) 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- Message-Id: {D06FF75E-9614-4D26-BA77-9425A50AFCAD-at-uconn.edu} 5, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 19 -- To: microscopy {microscopy-at-microscopy.com} 5, 19 -- From: David Knecht {david.knecht-at-uconn.edu} 5, 19 -- Subject: objective NA 5, 19 -- Date: Thu, 14 Jun 2007 13:40:34 -0400 5, 19 -- X-Mailer: Apple Mail (2.752.3) 5, 19 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information. 5, 19 -- X-UConn-MailScanner: Found to be clean 5, 19 -- X-UConn-MailScanner-SpamCheck: ==============================End of - Headers==============================
==============================Original Headers============================== 17, 27 -- From ph2-at-sprynet.com Thu Jun 14 13:47:28 2007 17, 27 -- Received: from elasmtp-galgo.atl.sa.earthlink.net (elasmtp-galgo.atl.sa.earthlink.net [209.86.89.61]) 17, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EIlRMm001829 17, 27 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 13:47:27 -0500 17, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 17, 27 -- s=dk20050327; d=sprynet.com; 17, 27 -- b=k5Yb3tsTpncKy3r432AnaZ79IlLFh7a51WJczzhv458UQrfX/VW6pVWngqFlZjHe; 17, 27 -- h=Received:From:To:Cc:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:X-MimeOLE:In-Reply-To:Message-ID:X-ELNK-Trace:X-Originating-IP; 17, 27 -- Received: from [75.61.18.94] (helo=user915fa8f284) 17, 27 -- by elasmtp-galgo.atl.sa.earthlink.net with asmtp (Exim 4.34) 17, 27 -- id 1HyuLv-0003Av-6M; Thu, 14 Jun 2007 14:47:27 -0400 17, 27 -- From: "Tony Havics" {ph2-at-sprynet.com} 17, 27 -- To: {david.knecht-at-uconn.edu} 17, 27 -- Cc: "Micrscopy Listserve" {microscopy-at-microscopy.com} 17, 27 -- Subject: RE: [Microscopy] objective NA 17, 27 -- Date: Thu, 14 Jun 2007 14:47:25 -0400 17, 27 -- MIME-Version: 1.0 17, 27 -- Content-Type: text/plain; 17, 27 -- charset="us-ascii" 17, 27 -- Content-Transfer-Encoding: 7bit 17, 27 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 17, 27 -- Thread-Index: Aceuq8mzPo2O3LLmSLSfM+5NwCzYQAAB8scA 17, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 17, 27 -- In-Reply-To: {200706141745.l5EHjOaj029640-at-ns.microscopy.com} 17, 27 -- Message-ID: {E1HyuLv-0003Av-6M-at-elasmtp-galgo.atl.sa.earthlink.net} 17, 27 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f9c4844ad2770a37891aa2f28f0fc702dd350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 17, 27 -- X-Originating-IP: 75.61.18.94 ==============================End of - Headers==============================
Thanks for the reply and to the others who have replied.
More data.
Gas is industrial welding Ar run through a Matheson molecular sieve (to dry and filter). SEM chamber uses industrial N2 also run through a molecular sieve.
Specimens are put in SEM chamber via Fjeld M-100 specimen load lock. This unit is pumped with small oil pump and turbo. Main SEM door is rarely opened. I have to check load lock pumps to see if it really is an oil roughing pump. I thought I got a dry unit for this too.
Pd target is from Refining Systems Las Vegas and is 99.5% pure. Trace elements do not include C. Coater is only used by myself. Chamber of coater is stainless steel (so it seems--but metal nevertheless). Only visible seal is the L ring rubber, or whatever, at the top.
This C issue just came up while trying to quant TaN on Si. It showed C that should not be there. So now I wonder about all spectra work and quants that include C. I can't think of a way to narrow down where the C is coming from and how to negate it. I will try cleaning the stubs and also try other stub types.
Exactly what are you saying about the TEM grid? The procedure is not clear to me. Are you saying I should coat it with Pd? Then what? I have STEM but not TEM.
gary g.
At 10:13 AM 6/14/2007, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 14, 20 -- From gary-at-gaugler.com Thu Jun 14 13:52:46 2007 14, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5EIqjCh013403 14, 20 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 13:52:46 -0500 14, 20 -- Message-Id: {200706141852.l5EIqjCh013403-at-ns.microscopy.com} 14, 20 -- Received: (qmail 12754 invoked from network); 14 Jun 2007 11:52:45 -0700 14, 20 -- Received: by simscan 1.1.0 ppid: 12751, pid: 12752, t: 0.2389s 14, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 14, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 14, 20 -- by qsmtp1 with SMTP; 14 Jun 2007 11:52:45 -0700 14, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 14, 20 -- Date: Thu, 14 Jun 2007 11:51:22 -0800 14, 20 -- To: bozzola-at-siu.edu 14, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 14, 20 -- Subject: Re: [Microscopy] Re: Carbon "contamination" 14, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 14, 20 -- In-Reply-To: {200706141813.l5EIDR6m013099-at-ns.microscopy.com} 14, 20 -- References: {200706141813.l5EIDR6m013099-at-ns.microscopy.com} 14, 20 -- Mime-Version: 1.0 14, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-27D32FCC ==============================End of - Headers==============================
Dear All, I did some EDS spectra on small steel particles. Specimen is uncoated on a adhesive C-tab. Energy had been 15 KV. I used a Roentec SLEW window detector. See images and spectra at www.elektronenmikroskopie.info/eds
Is there any other explaination for the titan peak to show up rather than it really is there in the specimen? Is there any possibility for fluorescense? Sorry in advance if this question is too simple for the list. I am not very experienced in interpreting eds spectra...
My first impression is that the titanium is real. It would be a little surprising for a regular stainless steel, but I see you also have cobalt peaks present. The peak around 7 kV is too intense to be only Fe K-beta. Co K-alpha is at 6.93 kV. You have a somewhat unusual specimen and Ti might be in order.
I don't know your x-ray system. I would highly recommend deconvoluting with the elements you know to be present and then examining the residuals from the fit. (Hopefully your system can show you the residuals. They are very helpful.) See what peaks or portions thereof are unaccounted for. I have had several different flavors of EDS systems over the years. They generally do a fair job of deconvolution _if_ you give them the right elements to begin with. For example, if I have a small mess around 2.3 kV and I let the EDS work with both S-K and Mo-L and Pb-M, it will generally give me a fair idea of which element is present if I have counted enough to well define my peaks.
The corollary is if you give the wrong elements or let the EDS pick the wrong elements on its own, you can get some quite screwy answers. Mo can turn into S. Ba can turn into Ti and Ag can turn into Ar.
Warren
-----Original Message----- X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de] Sent: Thursday, June 14, 2007 2:16 PM To: wesaia-at-iastate.edu
Dear All, I did some EDS spectra on small steel particles. Specimen is uncoated on a adhesive C-tab. Energy had been 15 KV. I used a Roentec SLEW window detector. See images and spectra at www.elektronenmikroskopie.info/eds
Is there any other explaination for the titan peak to show up rather than it really is there in the specimen? Is there any possibility for fluorescense? Sorry in advance if this question is too simple for the list. I am not very experienced in interpreting eds spectra...
HPD from EDAX works okay at low energy, but the labelling is not as sharp.
regards,
Jim
PS: OoO away.......
} From mail-at-ns.microscopy.com Thu Jun 14 12:52:28 2007 } Date: Thu, 14 Jun 2007 11:55:33 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: gary-at-gaugler.com } Reply-to: gary-at-gaugler.com } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] Carbon "contamination" } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Greetings all--I am seeking input on what appears } to be Carbon contamination. Here is the situation. } } Take a Pella 16111-9 stub out of bag and put on } holder. Take another stub out of bag and sputter } coat with Pd and put on holder. Do EDS on both. } } un-coated: } wt% at% } C 7.5 15 } O 4.5 7 } Al 88 78 } } coated: } C 23 38 } O 6.5 8 } Al 71 53 } } SEM is Zeiss Supra 55VP with Edwards XDS10 dry } scroll pump and turbo. Coater is Denton Desk IV } with Edwards XDS5 and turbo. } } The goal of using non-oil pumps was to reduce hydrocarbon } contamination. So, where is the C coming from? Nothing } has been done to the scroll pumps since new. There are } kits for repairing them but when is this necessary and } what would indicate that it be done? Would high C } be an indicator? } } I'm stumped on this one. } } Any ideas? } } gary g. } } } ==============================Original Headers============================== } 10, 17 -- From gary-at-gaugler.com Thu Jun 14 11:54:47 2007 } 10, 17 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) } 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5EGslUC027192 } 10, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 11:54:47 -0500 } 10, 17 -- Message-Id: {200706141654.l5EGslUC027192-at-ns.microscopy.com} } 10, 17 -- Received: (qmail 15711 invoked from network); 14 Jun 2007 09:54:47 -0700 } 10, 17 -- Received: by simscan 1.1.0 ppid: 15708, pid: 15709, t: 0.1452s } 10, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 } 10, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) } 10, 17 -- by qsmtp4 with SMTP; 14 Jun 2007 09:54:46 -0700 } 10, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 10, 17 -- Date: Thu, 14 Jun 2007 09:54:46 -0800 } 10, 17 -- To: MSA listserver {microscopy-at-microscopy.com} } 10, 17 -- From: Gary Gaugler {gary-at-gaugler.com} } 10, 17 -- Subject: Carbon "contamination" } 10, 17 -- Mime-Version: 1.0 } 10, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-20B34597 } ==============================End of - Headers============================== }
==============================Original Headers============================== 13, 12 -- From jquinn-at-www.matscieng.sunysb.edu Thu Jun 14 14:57:15 2007 13, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 13, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EJvELi017035 13, 12 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 14:57:14 -0500 13, 12 -- Received: (from jquinn-at-localhost) 13, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id l5EJr1B21280; 13, 12 -- Thu, 14 Jun 2007 15:53:01 -0400 13, 12 -- Date: Thu, 14 Jun 2007 15:53:01 -0400 13, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 13, 12 -- Message-Id: {200706141953.l5EJr1B21280-at-www.matscieng.sunysb.edu} 13, 12 -- To: gary-at-gaugler.com, microscopy-at-microscopy.com 13, 12 -- Subject: re: Pd two main M lines ==============================End of - Headers==============================
Do you observe the same effect with all detectors, (ie. A back scattered electron detector)? You might be having a problem with your secondary electron detector.
On 6/14/07 9:51 AM, "laable-at-solutia.com" {laable-at-solutia.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } To All Listers, } } I have recently been having problems with my field emission scope lately at } magnifications above 50KX. The image seems to swim and sparkles are seen and } the focus boundaries. A slow scan image impossible to acquire. The ground is } suspected. It is currently grounded to the building at the same location as } all the rest of the analytical equipment. Our electricians are in the process } of rerunning a separate ground for the scope. They are going to put an eight } foot copper rod in the earth outside of my lab drill hole in the wall for } wires to go through and attach it to the ground wires to the back of the } scope. I was wondering how the rest of have run the grounds for your scopes. } Any advice would be appreciated. } } Lori Ables } Solutia, Inc. } laable-at-solutia.com } } } This electronic mail message is intended exclusively for the individual or } entity to which it is addressed. } This message, together with any attachment, may contain Solutia confidential } and privileged information. } The recipient is hereby put on notice to treat the information as confidential } and privileged and to not disclose or use the information except as authorized } by Solutia. } Any unauthorized review, printing, retention, copying, disclosure, } distribution, retransmission, dissemination or other use of, or taking of any } action in reliance upon, this information by persons or entities other than } the intended recipient is prohibited. If you received this message in error, } please immediately contact the sender by reply email and delete all copies of } the material from any computer. Thank you for your cooperation. } } } ==============================Original Headers============================== } 6, 33 -- From laable-at-solutia.com Thu Jun 14 08:50:38 2007 } 6, 33 -- Received: from plmler9.mail.eds.com (plmler9.mail.eds.com } [199.228.142.117]) } 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l5EDoctm031340 } 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:38 -0500 } 6, 33 -- Received: from plmlir3.mail.eds.com (plmlir3-2.mail.eds.com } [199.228.142.133]) } 6, 33 -- by plmler9.mail.eds.com (8.13.8/8.13.8) with ESMTP id l5EDoYCV022081 } 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:36 -0500 } 6, 33 -- Received: from plmlir3.mail.eds.com (localhost.localdomain } [127.0.0.1]) } 6, 33 -- by plmlir3.mail.eds.com (8.13.8/8.12.10) with ESMTP id } l5EDoCXS028914 } 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:12 -0500 } 6, 33 -- Received: from mailgw2.solutia.com ([205.191.166.96]) } 6, 33 -- by plmlir3.mail.eds.com (8.13.8/8.12.10) with ESMTP id } l5EDoBND028887 } 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 08:50:12 -0500 } 6, 33 -- Received: from USAHMSLSOIEX2.soi.dir.solutia.com ([205.191.166.99]) } by mailgw2.solutia.com with Microsoft SMTPSVC(6.0.3790.1830); Thu, 14 Jun 2007 } 09:50:11 -0400 } 6, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.2826 } 6, 33 -- Content-Class: urn:content-classes:message } 6, 33 -- MIME-Version: 1.0 } 6, 33 -- Content-Type: text/plain; } 6, 33 -- charset="us-ascii" } 6, 33 -- Subject: SEM Ground } 6, 33 -- Date: Thu, 14 Jun 2007 09:50:08 -0400 } 6, 33 -- Message-ID: } {7B97BC1F2986504EABA8A0B1C780A5C301E77C67-at-USAHMSLSOIEX2.soi.dir.solutia.com} } 6, 33 -- Importance: normal } 6, 33 -- Priority: normal } 6, 33 -- X-MS-Has-Attach: } 6, 33 -- X-MS-TNEF-Correlator: } 6, 33 -- Thread-Topic: SEM Ground } 6, 33 -- thread-index: AceuiupcKKVbl+U3T3uHsV9HIQBKdA== } 6, 33 -- From: "Ables, Lori A" {laable-at-solutia.com} } 6, 33 -- To: {Microscopy-at-Microscopy.Com} } 6, 33 -- X-OriginalArrivalTime: 14 Jun 2007 13:50:11.0422 (UTC) } FILETIME=[ED5ACFE0:01C7AE8A] } 6, 33 -- Content-Transfer-Encoding: 8bit } 6, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id l5EDoctm031340 } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 25 -- From david.r.hull-at-nasa.gov Thu Jun 14 15:21:18 2007 6, 25 -- Received: from ndjsbar02.ndc.nasa.gov (ndjsbar02.ndc.nasa.gov [198.120.25.39]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EKLHiD028905 6, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 15:21:18 -0500 6, 25 -- Received: from ndjsxgw01.ndc.nasa.gov (ndjsxgw01.ndc.nasa.gov [129.166.32.111]) 6, 25 -- by ndjsbar02.ndc.nasa.gov (Spam Firewall) with ESMTP 6, 25 -- id EF4BD283987; Thu, 14 Jun 2007 15:21:16 -0500 (CDT) 6, 25 -- Received: from NDJSEVS23B.ndc.nasa.gov ([129.166.32.224]) by ndjsxgw01.ndc.nasa.gov with Microsoft SMTPSVC(6.0.3790.1830); 6, 25 -- Thu, 14 Jun 2007 15:21:16 -0500 6, 25 -- Received: from 129.166.32.12 ([129.166.32.12]) by NDJSEVS23B.ndc.nasa.gov ([129.166.32.227]) via Exchange Front-End Server mail01.ndc.nasa.gov ([129.166.32.102]) with Microsoft Exchange Server HTTP-DAV ; 6, 25 -- Thu, 14 Jun 2007 20:21:16 +0000 6, 25 -- User-Agent: Microsoft-Entourage/11.3.3.061214 6, 25 -- Date: Thu, 14 Jun 2007 16:21:15 -0400 6, 25 -- Subject: Re: [Microscopy] SEM Ground 6, 25 -- From: "Hull, David R" {David.R.Hull-at-nasa.gov} 6, 25 -- To: {laable-at-solutia.com} , {Microscopy-at-microscopy.com} 6, 25 -- Message-ID: {C2971B7B.670E%David.R.Hull-at-nasa.gov} 6, 25 -- Thread-Topic: [Microscopy] SEM Ground 6, 25 -- Thread-Index: AceuwY68zWJTXhq0EdyiPgAKldSx3A== 6, 25 -- In-Reply-To: {200706141351.l5EDpen4032477-at-ns.microscopy.com} 6, 25 -- Mime-version: 1.0 6, 25 -- Content-type: text/plain; 6, 25 -- charset="US-ASCII" 6, 25 -- Content-transfer-encoding: 7bit 6, 25 -- X-OriginalArrivalTime: 14 Jun 2007 20:21:16.0722 (UTC) FILETIME=[8FC37120:01C7AEC1] ==============================End of - Headers==============================
Electron/Ion Beam Instrument Engineer The University of Oregon's Center for Advanced Materials Characterization in Oregon (CAMCOR) is seeking applications for a full time staff position to begin September 2007. A strong background in maintaining, trouble shooting and upgrading electron/ion beam instruments and associated high voltage, vacuum, mechanical and electrical systems is required. Experience with x-ray diffraction instrumentation is also desirable. Salary range $60K-90K commensurate with experience.
This position will be located in the new Lorry Lokey Integrated Science Laboratory, a state of the art nano and micro science analytical instrument facility designed specifically for exceptional nano-science performance. It will house the latest electron, ion and x-ray beam instrumentation available including a Zeiss Ultra TFEM, FEI Quanta 200 E-SEM, Cameca SX50 and SX100 microprobes, Ion-TOF SIMS, Philips Auger Spectrometer, Philips TEM, Kratos XPS and various assorted coaters, etchers, and other vacuum deposition systems.
The successful candidate with have a BS in a beam microscopy related field and an extensive background in instrument field service with significant practical experience troubleshooting high vacuum electron and ion beam instrumentation at both the system and PC board levels. Must be able to read and understand schematics for electronic circuits and systems. The successful applicant will be involved in modifying/improving instrumentation capabilities to enable the equipment to more fully support unique research needs and will be expected to work intimately with the scientific staff and research faculty. We seek candidates with a demonstrated commitment to working effectively with students, faculty and staff from diverse backgrounds.
Interested persons should send a resume with a detailed description of work experience and skills, and arrange for two letters of recommendation to be sent to: CAMCOR Instrument Engineer Search Committee, 1253 University of Oregon, Eugene, OR, 97403-1253. To be assured of full consideration, application materials must be received by July 31, 2007, but the search will remain open until the position is filled. For further information, contact John Donovan (donovan-at-uoregon.edu).
University of Oregon is an AA/EEO employer committed to cultural diversity.
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Materials Engineer job opening -- Bodycote HIP (Hot Isostatic Pressing) in Andover, Massachusetts, has an immediate opening for an entry-level B.S. or M.S. materials or metallurgical engineer for our Technical Services group. The ideal candidate will have a strong interest and/or experience in metallography, microscopy and failure analysis. He/she will interact with both internal and external customers, so good communication skills are a must. A solid background in thermodynamics and physical metallurgy is also desired. Powder metallurgy, heat-treating and/or foundry knowledge is also a plus.
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==============================Original Headers============================== 8, 31 -- From Jane.LaGoy-at-Bodycote.com Thu Jun 14 15:36:47 2007 8, 31 -- Received: from mail161.messagelabs.com (mail161.messagelabs.com [216.82.253.115]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5EKakOL019724 8, 31 -- for {Microscopy-at-MSA.Microscopy.com} ; Thu, 14 Jun 2007 15:36:46 -0500 8, 31 -- X-VirusChecked: Checked 8, 31 -- X-Env-Sender: Jane.LaGoy-at-Bodycote.com 8, 31 -- X-Msg-Ref: server-2.tower-161.messagelabs.com!1181853404!3236203!1 8, 31 -- X-StarScan-Version: 5.5.12.11; banners=-,-,- 8, 31 -- X-Originating-IP: [209.235.7.177] 8, 31 -- Received: (qmail 22703 invoked from network); 14 Jun 2007 20:36:45 -0000 8, 31 -- Received: from 177-209.235.7.appsitehosting.com (HELO VM200EXC02.ad.bodycote.na) (209.235.7.177) 8, 31 -- by server-2.tower-161.messagelabs.com with SMTP; 14 Jun 2007 20:36:45 -0000 8, 31 -- Received: from VM200EXC01.ad.bodycote.na ([10.200.20.9]) by VM200EXC02.ad.bodycote.na with Microsoft SMTPSVC(6.0.3790.3959); 8, 31 -- Thu, 14 Jun 2007 16:36:44 -0400 8, 31 -- Content-Class: urn:content-classes:message 8, 31 -- MIME-Version: 1.0 8, 31 -- Content-Type: text/plain; 8, 31 -- charset="us-ascii" 8, 31 -- Subject: Materials Engineer position opening 8, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.3959 8, 31 -- Date: Thu, 14 Jun 2007 16:36:45 -0400 8, 31 -- Message-ID: {412949C8D462414B9BE7A0921A9C841608FEAA-at-VM200EXC01.ad.bodycote.na} 8, 31 -- X-MS-Has-Attach: 8, 31 -- X-MS-TNEF-Correlator: 8, 31 -- Thread-Topic: Materials Engineer position opening 8, 31 -- thread-index: Aceuw7lRzjm4nwJXQiG7pcIovLPqkg== 8, 31 -- From: "Jane LaGoy" {Jane.LaGoy-at-Bodycote.com} 8, 31 -- To: {Microscopy-at-MSA.Microscopy.com} 8, 31 -- X-OriginalArrivalTime: 14 Jun 2007 20:36:44.0309 (UTC) FILETIME=[B8A5FC50:01C7AEC3] 8, 31 -- Content-Transfer-Encoding: 8bit 8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5EKakOL019724 ==============================End of - Headers==============================
The only possible overlap with C and Pd is at Pd Mz=0.286KeV. But at 5KV beam, I would expect this Pd peak to be quite low.
Then, it still does not explain why I see C on an un-coated stub. The suggestion to clean some is good and I will do that. I checked the specimen holder and it is loaded with organics and C--likely from machining.
I can also try X-Checker and do Cu at 5KV and then try a Si die. This would eliminate any low KeV peaks near C. If I still see C, then...??? The low KV is necessary to analyze TaN since the N is oddly bound to the Ta. Different (higher) KV gives different results. I've done routing EDS on TiN and it is quite different.
A possible coating factor is the Ar cylinder. It is steel and is about seven years old. I wonder if over time, it oozes C into the Ar? A 2,000psi tank seems to last forever.
gary g.
At 11:58 AM 6/14/2007, you wrote:
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==============================Original Headers============================== 14, 20 -- From gary-at-gaugler.com Thu Jun 14 15:53:30 2007 14, 20 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5EKrUTC031482 14, 20 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 15:53:30 -0500 14, 20 -- Message-Id: {200706142053.l5EKrUTC031482-at-ns.microscopy.com} 14, 20 -- Received: (qmail 10432 invoked from network); 14 Jun 2007 13:53:29 -0700 14, 20 -- Received: by simscan 1.1.0 ppid: 10426, pid: 10427, t: 0.1195s 14, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 14, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 14, 20 -- by qsmtp2 with SMTP; 14 Jun 2007 13:53:29 -0700 14, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 14, 20 -- Date: Thu, 14 Jun 2007 13:53:28 -0800 14, 20 -- To: jquinn-at-www.matscieng.sunysb.edu 14, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 14, 20 -- Subject: Re: [Microscopy] re: Pd two main M lines 14, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 14, 20 -- In-Reply-To: {200706141958.l5EJwkaB019860-at-ns.microscopy.com} 14, 20 -- References: {200706141958.l5EJwkaB019860-at-ns.microscopy.com} 14, 20 -- Mime-Version: 1.0 14, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-46F525F ==============================End of - Headers==============================
The thin Pd coating may enhance the M peaks over the L peaks
Run a simulation with WinXRay at 5kV. See fourth image at this URL: http://www.matscieng.sunysb.edu/temp/gg/
You will see two Pd-M peaks are nearly the size of the Pd-Lb peak. You can tweak the thickness to change the M/L ratio.
Also, with the weak signal, you may be seeing secondary emission from your polymer window.
Also, carbon is everywhere. Fact of life. HF etch a silicon a chip from a silicon wafer. Take a spectrum. That will be your low limit on cleanliness of carbon (not oxygen). You could also Shirake etch the Si chip.
JQ
} From gary-at-gaugler.com Thu Jun 14 16:50:19 2007 } X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } Date: Thu, 14 Jun 2007 13:53:28 -0800 } To: jquinn-at-www.matscieng.sunysb.edu } From: Gary Gaugler {gary-at-gaugler.com} } Subject: Re: [Microscopy] re: Pd two main M lines } Cc: MSA listserver {microscopy-at-microscopy.com} } In-Reply-To: {200706141958.l5EJwkaB019860-at-ns.microscopy.com} } References: {200706141958.l5EJwkaB019860-at-ns.microscopy.com} } Mime-Version: 1.0 } Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-46F525F } } Check out the spectra at } } http://www.gaugler.com/coatedstub.pdf } } The only possible overlap with C and Pd is } at Pd Mz=0.286KeV. But at 5KV beam, I would } expect this Pd peak to be quite low. } } Then, it still does not explain why I see } C on an un-coated stub. The suggestion to } clean some is good and I will do that. I } checked the specimen holder and it is loaded } with organics and C--likely from machining. } } I can also try X-Checker and do Cu at 5KV } and then try a Si die. This would eliminate } any low KeV peaks near C. If I still see C, } then...??? The low KV is necessary to analyze } TaN since the N is oddly bound to the Ta. Different } (higher) KV gives different results. I've done } routing EDS on TiN and it is quite different. } } A possible coating factor is the Ar cylinder. It } is steel and is about seven years old. I wonder if } over time, it oozes C into the Ar? A 2,000psi } tank seems to last forever. } } gary g. } } } At 11:58 AM 6/14/2007, you wrote: } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Gary and company } } } } http://www.matscieng.sunysb.edu/temp/gg/ } } } } The link will vanish is a few weeks. } } } } The three spectra are calculations. } } } } The first is for C-K and O-K with 100cts. } } } } The second is for Pd-M with 100cts. } } } } The third is them combined. } } } } You would see the same with a real sample. } } } } HPD from EDAX works okay at low energy, but } } the labelling is not as sharp. } } } } regards, } } } } Jim } } } } PS: OoO away....... } } } } } From mail-at-ns.microscopy.com Thu Jun 14 12:52:28 2007 } } } Date: Thu, 14 Jun 2007 11:55:33 -0500 } } } To: jquinn-at-www.matscieng.sunysb.edu } } } From: gary-at-gaugler.com } } } Reply-to: gary-at-gaugler.com } } } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } } } Subject: [Microscopy] Carbon "contamination" } } } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } } } X-lewp: MicroscopyListSpam NAGS } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } ---------------------------------------------------------------------------- } } } } } } Greetings all--I am seeking input on what appears } } } to be Carbon contamination. Here is the situation. } } } } } } Take a Pella 16111-9 stub out of bag and put on } } } holder. Take another stub out of bag and sputter } } } coat with Pd and put on holder. Do EDS on both. } } } } } } un-coated: } } } wt% at% } } } C 7.5 15 } } } O 4.5 7 } } } Al 88 78 } } } } } } coated: } } } C 23 38 } } } O 6.5 8 } } } Al 71 53 } } } } } } SEM is Zeiss Supra 55VP with Edwards XDS10 dry } } } scroll pump and turbo. Coater is Denton Desk IV } } } with Edwards XDS5 and turbo. } } } } } } The goal of using non-oil pumps was to reduce hydrocarbon } } } contamination. So, where is the C coming from? Nothing } } } has been done to the scroll pumps since new. There are } } } kits for repairing them but when is this necessary and } } } what would indicate that it be done? Would high C } } } be an indicator? } } } } } } I'm stumped on this one. } } } } } } Any ideas? } } } } } } gary g. } } }
==============================Original Headers============================== 11, 12 -- From jquinn-at-www.matscieng.sunysb.edu Thu Jun 14 16:21:21 2007 11, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5ELLL6Q011139 11, 12 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 16:21:21 -0500 11, 12 -- Received: (from jquinn-at-localhost) 11, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id l5ELI3921642; 11, 12 -- Thu, 14 Jun 2007 17:18:03 -0400 11, 12 -- Date: Thu, 14 Jun 2007 17:18:03 -0400 11, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 11, 12 -- Message-Id: {200706142118.l5ELI3921642-at-www.matscieng.sunysb.edu} 11, 12 -- To: gary-at-gaugler.com, microscopy-at-microscopy.com 11, 12 -- Subject: Re: [Microscopy] re: Pd two main M lines ==============================End of - Headers==============================
Our lab has a Buehler IsoMet slow saw (model #11-1180, apparently 1980s vintage) that has ceased functioning. I've tracked the problem down to broken gear teeth on the shaft perpendicular to the motor's drive shaft. Does anyone else have a dead or unused IsoMet that they wouldn't mind someone cannibalizing? We can pay shipping and/or modestly for the equipment/parts. We also don't have the instructions or specifications anymore. If someone else does and there are good part descriptions, I'd be interested in a fax or scan of that page to help track down a replacement part. Thanks in advance!
Cheers, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu Personal Website: http://umn.edu/~frah0010
Please note: Sometimes the University's spam filters are over- protective and reject wanted messages, especially from free or overseas accounts. If your message is rejected or you are concerned that you did not receive a reply from me, please feel free to try my alternate email account: elleryfrahm-at-mac.com
==============================Original Headers============================== 6, 17 -- From frah0010-at-umn.edu Thu Jun 14 18:58:38 2007 6, 17 -- Received: from mta-w2.tc.umn.edu (mta-w2.tc.umn.edu [134.84.119.6]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5ENwcCp025383 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 18:58:38 -0500 6, 17 -- Received: from [10.0.1.3] (c-75-72-182-252.hsd1.mn.comcast.net [75.72.182.252]) 6, 17 -- by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 18:58:38 -0500 (CDT) 6, 17 -- X-Umn-Remote-Mta: [N] c-75-72-182-252.hsd1.mn.comcast.net [75.72.182.252] #+TS+AU+HN 6, 17 -- Mime-Version: 1.0 (Apple Message framework v752.2) 6, 17 -- Content-Transfer-Encoding: 7bit 6, 17 -- Message-Id: {1862217B-D7B4-46CA-99B6-0819AFC25E13-at-umn.edu} 6, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 6, 17 -- To: microscopy-at-microscopy.com 6, 17 -- From: Ellery Frahm {frah0010-at-umn.edu} 6, 17 -- Subject: Buehler IsoMet 6, 17 -- Date: Thu, 14 Jun 2007 18:58:37 -0500 6, 17 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nmedvitz-at-nephrocor.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: nmedvitz-at-nephrocor.com Name: Neil Medvitz
Organization: Bostwick Laboratories
Title-Subject: [Filtered] Advantages of having two TEMs in the same location rather than having one EM in two locations
Question: I am writing justification for having two TEMs in the same location rather one EM in two locations. I have a lot of reasons but just want to make sure I am not overlooking anything. Could I please get some opinions?
Thanks to all who help, Neil
Neil Medvitz Electron Microscopy Technical Director
==============================Original Headers============================== 11, 12 -- From zaluzec-at-microscopy.com Thu Jun 14 19:18:48 2007 11, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5F0Im9L004972 11, 12 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 19:18:48 -0500 11, 12 -- Mime-Version: 1.0 11, 12 -- Message-Id: {p06240805c2978b531766-at-[206.69.208.22]} 11, 12 -- Date: Thu, 14 Jun 2007 19:18:47 -0500 11, 12 -- To: microscopy-at-microscopy.com 11, 12 -- From: nmedvitz-at-nephrocor.com (by way of MicroscopyListserver) 11, 12 -- Subject: viaWWW: Advantages of having two TEMs in the same location rather 11, 12 -- than having one EM in two locations 11, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
We are renovating our lab to install an FEI Tecnai G2 20TEM. As we think about the electrical needs, we wonder about the necessity of backing the system with an uninterrupted power supply and generator. My understanding is that in the event of a power outage, the system will shut down, the pneumatic valves will close and the microscope will survive. But I wonder about the support computers. I am asking for the advice of others in how they have addressed UPS for their modern TEM systems.
Many thanks,
Doug
Douglas R. Keene Assistant Investigator Micro-Imaging Center Shriners Hospital for Children 3102 S.W. Sam Jackson Park Road Portland, Oregon 97239 503-221-3434 drk-at-shcc.org
==============================Original Headers============================== 7, 22 -- From drk-at-SHCC.org Thu Jun 14 19:59:23 2007 7, 22 -- Received: from mail.SHCC.org (mail.shcc.org [64.213.211.202]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5F0xNPn017267 7, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jun 2007 19:59:23 -0500 7, 22 -- Received: from DRK2 (drk2.research.shcc.org [64.213.211.62]) 7, 22 -- by mail.SHCC.org (PMDF V6.3 #31473) 7, 22 -- with ESMTPA id {0JJN0052CJY49G-at-mail.SHCC.org} for Microscopy-at-Microscopy.Com; 7, 22 -- Thu, 14 Jun 2007 17:56:29 -0700 (PDT) 7, 22 -- Date: Thu, 14 Jun 2007 18:00:51 -0700 7, 22 -- From: Doug Keene {drk-at-SHCC.org} 7, 22 -- Subject: UPS for TEMs 7, 22 -- Sender: drk-at-SHCC.org 7, 22 -- To: Microscopy-at-Microscopy.Com 7, 22 -- Reply-to: drk-at-SHCC.org 7, 22 -- Message-id: {0JJN0052DJY49G-at-mail.SHCC.org} 7, 22 -- Organization: Shriners Hospitals for Children 7, 22 -- MIME-version: 1.0 7, 22 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 7, 22 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 7, 22 -- Content-type: text/plain; charset=us-ascii 7, 22 -- Content-transfer-encoding: 7BIT 7, 22 -- Thread-Index: Aceu6J48gStBi823SVWXipCrNp21ww== ==============================End of - Headers==============================
If power goes off, your gun goes off. Of course, if it is the same as an FESEM.
My solution is two 8KVA dual redundant Liebert nFinity dual conversion UPS units. Split the load between the two units. The SEM is on one unit while the EDS, PCs, chiller and other stuff are on the other unit. With redundant converters and controllers, an 8KVA unit does 4KVA with half of a unit. If one portion fails, the other half works. Fix the failed unit and back to 100% redundant.
Bad power is a big problem here in CA. Dual conversion is a huge benefit of these UPS. Steady, constant and always there. With full set of batteries, I have about 280 minutes of UPS backup for each UPS. each unit sees about 22% load.
Full complement of batteries (no expansion unit), and redundant controllers runs about $10K per unit. They are rock solid. For about $300 each, you can hook them to a LAN and monitor what is going on with power. You will be surprised. Or, manually view the event log.
Liebert makes higher capacity units but these work for my application.
gary g.
At 05:00 PM 6/14/2007, you wrote:
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==============================Original Headers============================== 13, 20 -- From gary-at-gaugler.com Fri Jun 15 00:08:06 2007 13, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5F585ce000483 13, 20 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 00:08:06 -0500 13, 20 -- Message-Id: {200706150508.l5F585ce000483-at-ns.microscopy.com} 13, 20 -- Received: (qmail 1410 invoked from network); 14 Jun 2007 22:08:05 -0700 13, 20 -- Received: by simscan 1.1.0 ppid: 1407, pid: 1408, t: 0.1095s 13, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 13, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 13, 20 -- by qsmtp3 with SMTP; 14 Jun 2007 22:08:05 -0700 13, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 20 -- Date: Thu, 14 Jun 2007 22:08:01 -0800 13, 20 -- To: drk-at-SHCC.org 13, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 13, 20 -- Subject: Re: [Microscopy] UPS for TEMs 13, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 13, 20 -- In-Reply-To: {200706150100.l5F10oO5020068-at-ns.microscopy.com} 13, 20 -- References: {200706150100.l5F10oO5020068-at-ns.microscopy.com} 13, 20 -- Mime-Version: 1.0 13, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-730C7E22 ==============================End of - Headers==============================
I agrees with the different comments about Pd Mz line overlapping on Carbon K. I had the same stuff with new polished and ion etched Pt standards, where I found that intrusive carbon... which was Pd-N line ! And my software don't have the N lines tabulated ! I had too the probleme with the Pd-M in Pd/Fe alloys.
Added to the overlapping probleme, in some situation one have catalysis effect on carbon cracking. The carbon peak comes really from carbon, but groes during the spectrum acquisition ! In such case you should see these famous hated black squares after the analysis...
About the uncoated sample, a collegue now retired said me never to use plastic bags or plasctic boxes for sample storage. Most plastics outgas solvent continusly and polluate all the surfaces with solvent. He used only glass ware. One need only a monolayer of hydrocarbon to have some contamination ! People who make Auger spectroscopy know that one must clean away the carbon before one see something else !
I've made some test, and my conclusion is that in most cases, the sample and the sample hodler bring with them the major source of contamination. The second source is the rotary-vane pump, and the last is the diff pump.
The two ways to limit contamination are first to use a lN2 cold trap very near of the sample, in front of the OL, or beside , or around it (one must have a fast entry lock), and second to put all parts which comes to air, the holder and the sample in a plasma cleaner, before putting them in the SEM (one must have much monney to buy one !). A simple glow discharge in air can be suffisant, but is not very reproductible.
About the Supra configuration, just a coment : as we bought our first FE-SEM, I saw in the test round that the Supra (in that time it was a 1530) was the SEM which had the most contamination from the five SEM we have seen. It was one raison for an other choice. Now we have bought a Supra 40 since a few month, for E-beam lithography, a application where one know that contamination cannot be avoided (one put in samples with a fresh coating of PMMA or other durty stuffs !), I have seen that, before performing any litho work, that contamination question was still the same. People from Leo/Zeiss say that the good performences of their SE-decteor reveal very thin contamination levels. My thought is that the vacuum demand isn't high enough. One cannot vent to air inocently a vaccum chamber with a so big specific surface and a turbo pump at each sample change without consequences. With the time one catch organic contamination from the environnement in the SEM itself. This can be one more source of contamination.
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
gary-at-gaugler.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Greetings all--I am seeking input on what appears } to be Carbon contamination. Here is the situation. } } Take a Pella 16111-9 stub out of bag and put on } holder. Take another stub out of bag and sputter } coat with Pd and put on holder. Do EDS on both. } } un-coated: } wt% at% } C 7.5 15 } O 4.5 7 } Al 88 78 } } coated: } C 23 38 } O 6.5 8 } Al 71 53 } } SEM is Zeiss Supra 55VP with Edwards XDS10 dry } scroll pump and turbo. Coater is Denton Desk IV } with Edwards XDS5 and turbo. } } The goal of using non-oil pumps was to reduce hydrocarbon } contamination. So, where is the C coming from? Nothing } has been done to the scroll pumps since new. There are } kits for repairing them but when is this necessary and } what would indicate that it be done? Would high C } be an indicator? } } I'm stumped on this one. } } Any ideas? } } gary g. } } } ==============================Original Headers============================== } 10, 17 -- From gary-at-gaugler.com Thu Jun 14 11:54:47 2007 } 10, 17 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) } 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5EGslUC027192 } 10, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 11:54:47 -0500 } 10, 17 -- Message-Id: {200706141654.l5EGslUC027192-at-ns.microscopy.com} } 10, 17 -- Received: (qmail 15711 invoked from network); 14 Jun 2007 09:54:47 -0700 } 10, 17 -- Received: by simscan 1.1.0 ppid: 15708, pid: 15709, t: 0.1452s } 10, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 } 10, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) } 10, 17 -- by qsmtp4 with SMTP; 14 Jun 2007 09:54:46 -0700 } 10, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 10, 17 -- Date: Thu, 14 Jun 2007 09:54:46 -0800 } 10, 17 -- To: MSA listserver {microscopy-at-microscopy.com} } 10, 17 -- From: Gary Gaugler {gary-at-gaugler.com} } 10, 17 -- Subject: Carbon "contamination" } 10, 17 -- Mime-Version: 1.0 } 10, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-20B34597 } ==============================End of - Headers============================== }
==============================Original Headers============================== 13, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Jun 15 02:33:10 2007 13, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.157]) 13, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5F7XArb014857 13, 29 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 02:33:10 -0500 13, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 13, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id l5F7X8Kj011806 13, 29 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 09:33:09 +0200 (CEST) 13, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 13, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 69E867E40A1 13, 29 -- for {Microscopy-at-Microscopy.Com} ; Fri, 15 Jun 2007 09:32:26 +0200 (CEST) 13, 29 -- Message-ID: {467240A5.7060607-at-ipcms.u-strasbg.fr} 13, 29 -- Date: Fri, 15 Jun 2007 09:32:53 +0200 13, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 13, 29 -- User-Agent: Thunderbird 1.5.0.12 (X11/20070604) 13, 29 -- MIME-Version: 1.0 13, 29 -- To: Microscopy-at-microscopy.com 13, 29 -- Subject: Re: [Microscopy] Carbon "contamination" 13, 29 -- References: {200706141700.l5EH0QJH003815-at-ns.microscopy.com} 13, 29 -- In-Reply-To: {200706141700.l5EH0QJH003815-at-ns.microscopy.com} 13, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 29 -- Content-Transfer-Encoding: 8bit 13, 29 -- X-IPCMS-MailScanner: Found to be clean 13, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 13, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.157]); Fri, 15 Jun 2007 09:33:09 +0200 (CEST) 13, 29 -- X-Virus-Scanned: ClamAV 0.88.7/3424/Fri Jun 15 03:30:29 2007 on mr7.u-strasbg.fr 13, 29 -- X-Virus-Status: Clean 13, 29 -- X-Spam-Status: No, score=-0.3 required=5.0 tests=AWL autolearn=disabled 13, 29 -- version=3.1.8 13, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr7.u-strasbg.fr ==============================End of - Headers==============================
In my former mail, in answer to Gary's question, I have said somthing from the use of the plasma cleaner. I take the opportunity to comme with a new question.
Did some one still build a plasma cleaner in the SEM itself, or better in the fast entry lock from the SEM. This would be the most drasctic way to avoid the contamination coming with the sample/sample holder.
If some has tried, or know from some tests...
Thanks
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Dear Jaques, have a look at http://www.semclean.com It seems to work greatly and is used - to my knowledge - by some major sem manufacturer - in Europe...
Best regards, Stefan Diller
----- Original Message ----- X-from: {jacques.faerber-at-ipcms.u-strasbg.fr} To: {stefan.diller-at-t-online.de} Sent: Friday, June 15, 2007 9:44 AM
Hi all
Receving just my mail from this morning, I see that I that I have made an error in the first paragraph. It is the Pt-N line and not the Pd-N line, which interfer, like the Pd-M.
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Yes, a plasma cleaner for an EM Chamber is a commerical product. There have been several papers written on this from both the SEM and TEM standpoint.
You should look at the following WWW site from XEI Scientific for the SEM info. They have a number of papers on-line to download.
http://www.evactron.com/
Disclaimer: Argonne National Lab, my daytime employer holds the basic patent on this technology for EM applications and licenses it to a number of commerical organizations, XEI being one of them.
Nestor Your Friendly Neighborhood SysOp.
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Hi all
In my former mail, in answer to Gary's question, I have said somthing from the use of the plasma cleaner. I take the opportunity to comme with a new question.
Did some one still build a plasma cleaner in the SEM itself, or better in the fast entry lock from the SEM. This would be the most drasctic way to avoid the contamination coming with the sample/sample holder.
If some has tried, or know from some tests...
Thanks
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des MatÈriaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Numerical Aperture: This is a critical value that indicates the light acceptance angle, which in turn determines the light gathering power, the resolving power, and depth of field of the objective.
Thus the higher the resolving power (NA) of an objective, the lower the depth of field (and so working distance). So a high NA objective may not suite all applications. Indeed a typical high magnification objective focus point will only just penetrate through a standard 0.17mm glass coverslip. An air objective will have a far greater (relatively) depth of field and thus a far longer working distance, particularly an LD 'long working distance' variety - but the image quality will be noticeably poorer (not a problem though if you can't see squat with your high NA short working distance oil immersion lens). This is all to do with airey disks and stuff, [and also a lot to do with the price of the objective and how well it's made]. Image contrast, as well as resolution, is also an important consideration here.
'Working distance' correction Collar
An adjustment [correction] collar for cover glass thickness (not NA) is sometimes provided on high-NA microscope objective lenses. Rotation of the collar adjusts the height of certain lens elements in the objective lens to compensate for variations in coverslip thickness or immersion media. Thus it provides an adjustable working distance. At high NAs, even a small deviation of the coverslip thickness (by as little as a few micrometers in some cases), or refractive index of the immersion medium from the designated standard, can introduce significant aberrations.
Variable NA collar
Other objectives specifically designed for transmitted light fluorescence and darkfield imaging are sometimes equipped with an internal iris diaphragm that allows for adjustment of the effective numerical aperture [NA]. A 60x apochromat objective can have a numerical aperture of 1.4, one of the highest attainable in modern microscopes using immersion oil as an imaging medium.
Variable Numerical Aperture Objectives: Specimens with unusually high fluorescence quantum yields and/or very bright darkfield specimens often induce image flare by light emitted from areas outside the focal plane. To compensate for this artefact, manufacturers offer high numerical aperture objectives that are equipped with an internal iris diaphragm to increase image contrast during photomicrography or digital imaging. Opening or closing the iris diaphragm determines the size of the objective rear aperture yielding a variable numerical aperture range between 0.5 and the objective's upper limit (up to 1.35-1.4 with apochromatic objectives). Although iris diaphragms were once utilized in a wide variety of objective designs, modern variable numerical aperture objectives are usually at the high end (60x to 150x) of the magnification range.
A variable NA collar on an oil immersion thus help with contrast enhancement when set to low NA, but it's not going to help it in any way achieve the long working distance of a dry LD objective of the same magnification. I must admit that I don't bother too much with the physics equations when looking down the microscope, as any decent microscope rep should be able provide you with a set of objectives to assess which one suites your needs best before you buy. Typically you would go for the highest NA you can for the given working distance required. Manufacturer's websites provide the objectives working distance (in mm) and NA info. Modern ultra-high NA objectives are required for TIRF applications and hi-res DIC contrast enhancement.
To achieve higher NA than 0.95 for objectives they have to be used with immersion media between front lens and specimen. Oil or water is mostly used for that purpose. Because objectives have to be specially designed for this, the type of immersion media is always mentioned on the objective like on the UPlanFLN 60x/1.25 Oil Iris objective. This objective needs oil as an immersion media and can not produce good image quality without it.
High NA objectives also collect more light and if you compare two objectives with fluorescent samples, the high NA objective should produce a noticeably brighter (and preferable) image. Again though the high NA objective is generally far more expensive and it's better attention to quality optics/coatings will also be factor in it's superior performance. An objective also needs to be PLAN Apochromat and fluorescence friendly for quality fluorophore imaging.
The only problem with using our variable NA collar objectives here is that we use inverted optics and have no idea where the NA collar is actually set [e.g. if it's moved accidentally or the previous user has adjusted it]. I do put a chart on the wall telling users which way to turn the NA collar for highest NA (viewed from above), but I'm not sure all users bother with this. I have noticed slight changes in um/pixel calibration (less than 10% difference, and it's linear) between the two NA extremes on some objectives.
To be told more than you probably want to know about resolving power and NA see:
----------------------- Dr Keith J Morris [Formerly] Imaging Facilities Manager Cell Biology Institute of Ophthalmology UCL, London EC1V 9EL
-----Original Message----- X-from: david.knecht-at-uconn.edu [mailto:david.knecht-at-uconn.edu] Sent: 14 June 2007 18:44 To: keith.morris-at-ucl.ac.uk
One can buy a 40-100x oil objective with an iris diaphram. What is the theoretical difference in performance between imaging with a low NA dry objective (.6NA for example) and an equivalent magnification, high NA oil objective with the iris partially closed to reduce the NA to .6? Should they be similar? Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 5, 19 -- From david.knecht-at-uconn.edu Thu Jun 14 12:40:46 2007 5, 19 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5EHekrM018923 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 12:40:46 -0500 5, 19 -- Received: from [137.99.46.174] (d46h174.public.uconn.edu [137.99.46.174]) 5, 19 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id l5EHeffX009795 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 13:40:41 -0400 5, 19 -- Mime-Version: 1.0 (Apple Message framework v752.3) 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- Message-Id: {D06FF75E-9614-4D26-BA77-9425A50AFCAD-at-uconn.edu} 5, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 19 -- To: microscopy {microscopy-at-microscopy.com} 5, 19 -- From: David Knecht {david.knecht-at-uconn.edu} 5, 19 -- Subject: objective NA 5, 19 -- Date: Thu, 14 Jun 2007 13:40:34 -0400 5, 19 -- X-Mailer: Apple Mail (2.752.3) 5, 19 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information. 5, 19 -- X-UConn-MailScanner: Found to be clean 5, 19 -- X-UConn-MailScanner-SpamCheck: ==============================End of - Headers==============================
==============================Original Headers============================== 35, 24 -- From keith.morris-at-ucl.ac.uk Fri Jun 15 08:19:33 2007 35, 24 -- Received: from smtp3.global.net.uk (smtp3.global.net.uk [80.189.92.91]) 35, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5FDJWuD015640 35, 24 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 08:19:33 -0500 35, 24 -- Received: from 190.79.rb4.adsl.brightview.com ([80.189.79.190] helo=loungepc) 35, 24 -- by smtp3.global.net.uk with esmtp (Exim 4.66 (FreeBSD)) 35, 24 -- (envelope-from {keith.morris-at-ucl.ac.uk} ) 35, 24 -- id 1HzBi5-000Ls6-Lq 35, 24 -- for microscopy-at-microscopy.com; Fri, 15 Jun 2007 14:19:31 +0100 35, 24 -- From: "Keith Morris" {keith.morris-at-ucl.ac.uk} 35, 24 -- To: {microscopy-at-microscopy.com} 35, 24 -- References: {200706141744.l5EHiHGX025348-at-ns.microscopy.com} 35, 24 -- Subject: RE: [Microscopy] objective NA 35, 24 -- Date: Fri, 15 Jun 2007 14:19:27 +0100 35, 24 -- Message-ID: {000001c7af4f$cede49a0$0301a8c0-at-loungepc} 35, 24 -- MIME-Version: 1.0 35, 24 -- Content-Type: text/plain; 35, 24 -- charset="us-ascii" 35, 24 -- Content-Transfer-Encoding: 7bit 35, 24 -- X-Mailer: Microsoft Office Outlook 11 35, 24 -- Thread-Index: Aceuq65IN79W3iYhQV6BDQVD6ZJEJAAkxKsg 35, 24 -- In-Reply-To: {200706141744.l5EHiHGX025348-at-ns.microscopy.com} 35, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 35, 24 -- Authenticated-Sender: ==============================End of - Headers==============================
Only saw someone's response to this question last evening and did not get to respond immediately, but Gary has really hit a major point. It is more than just protecting against the power outage. Most - if not all - microscopes have all sorts of emergency backups which go in place to prevent any real damage. Power outages are not common, butd they are major irritations when they occur. A real problem is spikes in power - the dirty power Gary refers to.
When I took over my current position it took me 18 months to figure out that the separate and isolated power source for my lab was neither separate nor isolated. The department had even put in (before me) a nice Barrie anti vibration system - works great, didn't stop the instability problems. We finally figured out what was happening when it was noticed that the aberrations in beam and focus occurred whenever someone was doing gel electrophoresis in a bank of labs in the next hallway. When we put a monitor on the power in line we found over 130 spikes in power in excess of 5% over the normal 12hour daytime period.
Fluctuations in power - spikes - do occur regularly. Not just in California, but in Winnipeg, Montreal, London, New York, Tokyo, etc. Surge protectors kick in after the first cycle of a spike. As a result, they do not protect against spikes with your computers and other 'not a microscope' equipment, they . Other than the glowing little light and giving you 5 plugs from 1 they probably do nothing to help.
Damage occurs in the first spike. This is true of the EM also. Dirty lines mean pops in and out of focus. If you've ever seen one, you know what this is. Imagine the fresnel fringe popping in and out concentrically while you look at the image you are trying to see. It may not bother you when you look at a section at 10,000, but it will drive you nuts when looking at a virus at 100,000+.
I didn't put in a UPS at the time. 25 years ago the unit would have cost } 10,000 and needed it's own room. But when we moved a microscope recently during a lab consolidation I built into the cost $2500 to put in a UPS. The best time to get things from administrators is when you are building a new lab, moving, renovating, or putting in a new microscope. Even if you have to spend Gary's $10-12,000US to protect several microscopes, or my $2,200C to protect one microscope, it is worth it.
==============================Original Headers============================== 7, 20 -- From paul_hazelton-at-umanitoba.ca Fri Jun 15 08:40:21 2007 7, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5FDeLei027646 7, 20 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 08:40:21 -0500 7, 20 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 7, 20 -- (authenticated bits=0) 7, 20 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id l5FDeJTc001912; 7, 20 -- Fri, 15 Jun 2007 08:40:19 -0500 (CDT) 7, 20 -- Message-ID: {46729645.6020801-at-umanitoba.ca} 7, 20 -- Date: Fri, 15 Jun 2007 08:38:13 -0500 7, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 7, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 7, 20 -- X-Accept-Language: en-us, en 7, 20 -- MIME-Version: 1.0 7, 20 -- To: gary-at-gaugler.com 7, 20 -- Subject: Re: [Microscopy] Re: UPS for TEMs 7, 20 -- References: {200706150510.l5F5ALIw003878-at-ns.microscopy.com} 7, 20 -- In-Reply-To: {200706150510.l5F5ALIw003878-at-ns.microscopy.com} 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have the same saw, functioning, and still have the original manual that came with it. So if you'd like, I can send you a copy.
However, I would suggest that you just call Buehler. I'm sure thay have a .pdf file with the manual and would send it to you. Buehler has sent me manuals, calibration data, all kinds of information on older equipment that they manufactured. They are really top notch in this regard, at least that has been my experience.
dj
On Thu, 14 Jun 2007, frah0010-at-umn.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Microscopists, } } Our lab has a Buehler IsoMet slow saw (model #11-1180, apparently } 1980s vintage) that has ceased functioning. I've tracked the problem } down to broken gear teeth on the shaft perpendicular to the motor's } drive shaft. Does anyone else have a dead or unused IsoMet that they } wouldn't mind someone cannibalizing? We can pay shipping and/or } modestly for the equipment/parts. We also don't have the } instructions or specifications anymore. If someone else does and } there are good part descriptions, I'd be interested in a fax or scan } of that page to help track down a replacement part. Thanks in advance! } } Cheers, } Ellery } } -------------------- } Ellery E. Frahm } Research Fellow & Manager } Electron Microprobe Laboratory } University of Minnesota - Twin Cities } Department of Geology & Geophysics } Lab Website: http://probelab.geo.umn.edu } Personal Website: http://umn.edu/~frah0010 } } Please note: Sometimes the University's spam filters are over- } protective and reject wanted messages, especially from free or } overseas accounts. If your message is rejected or you are concerned } that you did not receive a reply from me, please feel free to try my } alternate email account: elleryfrahm-at-mac.com } } } ==============================Original Headers============================== } 6, 17 -- From frah0010-at-umn.edu Thu Jun 14 18:58:38 2007 } 6, 17 -- Received: from mta-w2.tc.umn.edu (mta-w2.tc.umn.edu [134.84.119.6]) } 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l5ENwcCp025383 } 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 18:58:38 } -0500 } 6, 17 -- Received: from [10.0.1.3] (c-75-72-182-252.hsd1.mn.comcast.net } [75.72.182.252]) } 6, 17 -- by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP } 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 18:58:38 } -0500 (CDT) } 6, 17 -- X-Umn-Remote-Mta: [N] c-75-72-182-252.hsd1.mn.comcast.net } [75.72.182.252] #+TS+AU+HN } 6, 17 -- Mime-Version: 1.0 (Apple Message framework v752.2) } 6, 17 -- Content-Transfer-Encoding: 7bit } 6, 17 -- Message-Id: {1862217B-D7B4-46CA-99B6-0819AFC25E13-at-umn.edu} } 6, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed } 6, 17 -- To: microscopy-at-microscopy.com } 6, 17 -- From: Ellery Frahm {frah0010-at-umn.edu} } 6, 17 -- Subject: Buehler IsoMet } 6, 17 -- Date: Thu, 14 Jun 2007 18:58:37 -0500 } 6, 17 -- X-Mailer: Apple Mail (2.752.2) } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 16 -- From dljones-at-bestweb.net Fri Jun 15 09:02:44 2007 7, 16 -- Received: from vms046pub.verizon.net (vms046pub.verizon.net [206.46.252.46]) 7, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5FE2iNB007254 7, 16 -- for {Microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 09:02:44 -0500 7, 16 -- Received: from localhost ([71.247.229.59]) 7, 16 -- by vms046.mailsrvcs.net (Sun Java System Messaging Server 6.2-6.01 (built Apr 7, 16 -- 3 2006)) with ESMTPA id {0JJO001NEKBXQX74-at-vms046.mailsrvcs.net} for 7, 16 -- Microscopy-at-microscopy.com; Fri, 15 Jun 2007 09:02:28 -0500 (CDT) 7, 16 -- Date: Fri, 15 Jun 2007 10:03:41 -0400 (Eastern Daylight Time) 7, 16 -- From: "David L. Jones" {dljones-at-bestweb.net} 7, 16 -- Subject: Re: [Microscopy] Buehler IsoMet 7, 16 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 7, 16 -- To: Microscopy-at-microscopy.com 7, 16 -- Message-id: {Pine.WNT.4.64.0706151003230.3116-at-H-F1} 7, 16 -- MIME-version: 1.0 7, 16 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Lots of useful information in the responses, but let me clarify the question. A colleague is using his stopped down 40x oil objective in order to visualize vesicles throughout the volume of the cell in one image rather than confocal sectioning. I am trying to understand why it works and whether it would work equivalently with a dry objective. Obviously the light cone from a 1.4 NA 40x oil objective (whether iris is closed or not) and a 40x long working distance dry objective are different. The depth of field varies with numerical aperture, so a 40x 1.4 oil immersion lens, should inherently have a shallower depth of focus. Therefore, it makes no intuitive sense to me that you would #1-increase the depth of focus, and #2- get the same results with a 40x oil 1.4 in which you stop down the iris to . 5NA, as a .5NA LWD dry lens. The excitation spot profile of the 40x oil objective is not going to enlarge as you stop down the iris with the same input light path. So I am trying to understand how the focal depth/resolution/xyz profile of the excitation/emission etc. is for fluorescence in comparing those two situations. Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
On Jun 15, 2007, at 9:19 AM, keith.morris-at-ucl.ac.uk wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi David, } } Numerical Aperture: This is a critical value that indicates the light } acceptance angle, which in turn determines the light gathering } power, the } resolving power, and depth of field of the objective. } } Thus the higher the resolving power (NA) of an objective, the lower } the } depth of field (and so working distance). So a high NA objective } may not } suite all applications. Indeed a typical high magnification } objective focus } point will only just penetrate through a standard 0.17mm glass } coverslip. An } air objective will have a far greater (relatively) depth of field } and thus a } far longer working distance, particularly an LD 'long working } distance' } variety - but the image quality will be noticeably poorer (not a } problem } though if you can't see squat with your high NA short working } distance oil } immersion lens). This is all to do with airey disks and stuff, [and } also a } lot to do with the price of the objective and how well it's made]. } Image } contrast, as well as resolution, is also an important consideration } here. } } } 'Working distance' correction Collar } } An adjustment [correction] collar for cover glass thickness (not } NA) is } sometimes provided on high-NA microscope objective lenses. Rotation } of the } collar adjusts the height of certain lens elements in the objective } lens to } compensate for variations in coverslip thickness or immersion } media. Thus it } provides an adjustable working distance. At high NAs, even a small } deviation } of the coverslip thickness (by as little as a few micrometers in some } cases), or refractive index of the immersion medium from the } designated } standard, can introduce significant aberrations. } } } Variable NA collar } } Other objectives specifically designed for transmitted light } fluorescence } and darkfield imaging are sometimes equipped with an internal iris } diaphragm } that allows for adjustment of the effective numerical aperture } [NA]. A 60x } apochromat objective can have a numerical aperture of 1.4, one of the } highest attainable in modern microscopes using immersion oil as an } imaging } medium. } } Variable Numerical Aperture Objectives: Specimens with unusually high } fluorescence quantum yields and/or very bright darkfield specimens } often } induce image flare by light emitted from areas outside the focal } plane. To } compensate for this artefact, manufacturers offer high numerical } aperture } objectives that are equipped with an internal iris diaphragm to } increase } image contrast during photomicrography or digital imaging. Opening or } closing the iris diaphragm determines the size of the objective rear } aperture yielding a variable numerical aperture range between 0.5 } and the } objective's upper limit (up to 1.35-1.4 with apochromatic objectives). } Although iris diaphragms were once utilized in a wide variety of } objective } designs, modern variable numerical aperture objectives are usually } at the } high end (60x to 150x) of the magnification range. } } A variable NA collar on an oil immersion thus help with contrast } enhancement } when set to low NA, but it's not going to help it in any way } achieve the } long working distance of a dry LD objective of the same } magnification. I } must admit that I don't bother too much with the physics equations } when } looking down the microscope, as any decent microscope rep should be } able } provide you with a set of objectives to assess which one suites } your needs } best before you buy. Typically you would go for the highest NA you } can for } the given working distance required. Manufacturer's websites } provide the } objectives working distance (in mm) and NA info. Modern ultra-high NA } objectives are required for TIRF applications and hi-res DIC contrast } enhancement. } } To achieve higher NA than 0.95 for objectives they have to be used } with } immersion media between front lens and specimen. Oil or water is } mostly used } for that purpose. Because objectives have to be specially designed } for this, } the type of immersion media is always mentioned on the objective } like on the } UPlanFLN 60x/1.25 Oil Iris objective. This objective needs oil as an } immersion media and can not produce good image quality without it. } } High NA objectives also collect more light and if you compare two } objectives } with fluorescent samples, the high NA objective should produce a } noticeably } brighter (and preferable) image. Again though the high NA objective is } generally far more expensive and it's better attention to quality } optics/coatings will also be factor in it's superior performance. An } objective also needs to be PLAN Apochromat and fluorescence } friendly for } quality fluorophore imaging. } } The only problem with using our variable NA collar objectives here } is that } we use inverted optics and have no idea where the NA collar is } actually set } [e.g. if it's moved accidentally or the previous user has adjusted } it]. I do } put a chart on the wall telling users which way to turn the NA } collar for } highest NA (viewed from above), but I'm not sure all users bother } with this. } I have noticed slight changes in um/pixel calibration (less than 10% } difference, and it's linear) between the two NA extremes on some } objectives. } } } To be told more than you probably want to know about resolving } power and NA } see: } } http://www.olympusmicro.com/primer/anatomy/specialobjectives.html } http://www.microscopy.olympus.eu/microscopes/About_Microscopy_7436.htm } [The resolving power] } http://www.charfac.umn.edu/glossary/c.html } http://www.oznet.ksu.edu/ed_agron645/lab/microscope_use.htm } http://www.olympusconfocal.com/theory/confocalobjectives.html } http://www.microscopyu.com/tutorials/java/objectives/nuaperture/ } index.html } http://www.microscopyu.com/articles/optics/objectivespecs.html } http://www.micro.magnet.fsu.edu/primer/java/aberrations/ } slipcorrection/index } .html } } Regards } } Keith } } ----------------------- } Dr Keith J Morris } [Formerly] Imaging Facilities Manager } Cell Biology } Institute of Ophthalmology } UCL, London EC1V 9EL } } } } } -----Original Message----- } X-from: david.knecht-at-uconn.edu [mailto:david.knecht-at-uconn.edu] } Sent: 14 June 2007 18:44 } To: keith.morris-at-ucl.ac.uk } Subject: [Microscopy] objective NA } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } One can buy a 40-100x oil objective with an iris diaphram. What is } the theoretical difference in performance between imaging with a low } NA dry objective (.6NA for example) and an equivalent magnification, } high NA oil objective with the iris partially closed to reduce the NA } to .6? Should they be similar? Thanks- Dave } } } Dr. David Knecht } Department of Molecular and Cell Biology } Co-head Flow Cytometry and Confocal Microscopy Facility } U-3125 } 91 N. Eagleville Rd. } University of Connecticut } Storrs, CT 06269 } 860-486-2200 } 860-486-4331 (fax) } } } } ==============================Original } Headers============================== } 5, 19 -- From david.knecht-at-uconn.edu Thu Jun 14 12:40:46 2007 } 5, 19 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu } [137.99.25.204]) } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l5EHekrM018923 } 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 12:40:46 } -0500 } 5, 19 -- Received: from [137.99.46.174] (d46h174.public.uconn.edu } [137.99.46.174]) } 5, 19 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id } l5EHeffX009795 } 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jun 2007 13:40:41 } -0400 } 5, 19 -- Mime-Version: 1.0 (Apple Message framework v752.3) } 5, 19 -- Content-Transfer-Encoding: 7bit } 5, 19 -- Message-Id: {D06FF75E-9614-4D26-BA77-9425A50AFCAD-at-uconn.edu} } 5, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; } format=flowed } 5, 19 -- To: microscopy {microscopy-at-microscopy.com} } 5, 19 -- From: David Knecht {david.knecht-at-uconn.edu} } 5, 19 -- Subject: objective NA } 5, 19 -- Date: Thu, 14 Jun 2007 13:40:34 -0400 } 5, 19 -- X-Mailer: Apple Mail (2.752.3) } 5, 19 -- X-UConn-MailScanner-Information: Contact UConn Help Desk } 860-486-4357 for more information. } 5, 19 -- X-UConn-MailScanner: Found to be clean } 5, 19 -- X-UConn-MailScanner-SpamCheck: } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 35, 24 -- From keith.morris-at-ucl.ac.uk Fri Jun 15 08:19:33 2007 } 35, 24 -- Received: from smtp3.global.net.uk (smtp3.global.net.uk } [80.189.92.91]) } 35, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l5FDJWuD015640 } 35, 24 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 } 08:19:33 -0500 } 35, 24 -- Received: from 190.79.rb4.adsl.brightview.com } ([80.189.79.190] helo=loungepc) } 35, 24 -- by smtp3.global.net.uk with esmtp (Exim 4.66 (FreeBSD)) } 35, 24 -- (envelope-from {keith.morris-at-ucl.ac.uk} ) } 35, 24 -- id 1HzBi5-000Ls6-Lq } 35, 24 -- for microscopy-at-microscopy.com; Fri, 15 Jun 2007 14:19:31 } +0100 } 35, 24 -- From: "Keith Morris" {keith.morris-at-ucl.ac.uk} } 35, 24 -- To: {microscopy-at-microscopy.com} } 35, 24 -- References: {200706141744.l5EHiHGX025348-at-ns.microscopy.com} } 35, 24 -- Subject: RE: [Microscopy] objective NA } 35, 24 -- Date: Fri, 15 Jun 2007 14:19:27 +0100 } 35, 24 -- Message-ID: {000001c7af4f$cede49a0$0301a8c0-at-loungepc} } 35, 24 -- MIME-Version: 1.0 } 35, 24 -- Content-Type: text/plain; } 35, 24 -- charset="us-ascii" } 35, 24 -- Content-Transfer-Encoding: 7bit } 35, 24 -- X-Mailer: Microsoft Office Outlook 11 } 35, 24 -- Thread-Index: Aceuq65IN79W3iYhQV6BDQVD6ZJEJAAkxKsg } 35, 24 -- In-Reply-To: {200706141744.l5EHiHGX025348-at-ns.microscopy.com} } 35, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 } 35, 24 -- Authenticated-Sender: } ==============================End of - } Headers==============================
==============================Original Headers============================== 6, 21 -- From david.knecht-at-uconn.edu Fri Jun 15 09:20:25 2007 6, 21 -- Received: from mail1.uits.uconn.edu (mail1.uits.uconn.edu [137.99.25.203]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5FEKPs1019064 6, 21 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 09:20:25 -0500 6, 21 -- Received: from [137.99.46.174] (d46h174.public.uconn.edu [137.99.46.174]) 6, 21 -- by mail1.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id l5FEKKOK018997 6, 21 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 10:20:20 -0400 6, 21 -- Mime-Version: 1.0 (Apple Message framework v752.3) 6, 21 -- In-Reply-To: {200706151319.l5FDJhjN015904-at-ns.microscopy.com} 6, 21 -- References: {200706151319.l5FDJhjN015904-at-ns.microscopy.com} 6, 21 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 21 -- Message-Id: {CAAE52D2-2A0E-419D-B0CD-A8D66B18E7C4-at-uconn.edu} 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- From: David Knecht {david.knecht-at-uconn.edu} 6, 21 -- Subject: Re: [Microscopy] RE: objective NA 6, 21 -- Date: Fri, 15 Jun 2007 10:20:18 -0400 6, 21 -- To: microscopy microscopy {microscopy-at-microscopy.com} 6, 21 -- X-Mailer: Apple Mail (2.752.3) 6, 21 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information. 6, 21 -- X-UConn-MailScanner: Found to be clean 6, 21 -- X-UConn-MailScanner-SpamCheck: ==============================End of - Headers==============================
The Inter/Micro 2007 conference, scheduled for July 9-13, 2007, is rapidly approaching!
Announcing the symposium's schedule for speakers:
2007 Schedule of Presentations
See http://www.mcri.org/2007ScheduleforSpeakers.pdf
Thursday Workshop:
'Working with Living Cells - Triumphs, Tribulations and Tragedies', Jeremy Pickett-Heaps of the School of Botany, University of Melbourne, & Brian J. Ford of Gonville & Caius College, Cambridge University.
Friday Workshop: 'Airborne & Settled Dust Particle Identification Workshop', Andrew A. Havics, pH2 LLC., Randy Boltin, MVA Scientific Consultants, & John D. Shane, Ph.D., PRO-LAB/SSPTM, Inc.
Tony
Ps Disclaimer - I receive no profits from this although I'm teaching part of the one workshop on Friday.
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
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==============================Original Headers============================== 18, 25 -- From ph2-at-sprynet.com Fri Jun 15 10:42:18 2007 18, 25 -- Received: from elasmtp-junco.atl.sa.earthlink.net (elasmtp-junco.atl.sa.earthlink.net [209.86.89.63]) 18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5FFgIph000332 18, 25 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 10:42:18 -0500 18, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 18, 25 -- s=dk20050327; d=sprynet.com; 18, 25 -- b=AgXTNIysJslzNcLufRKv9PZRYEs9nvlp4k8MzwWDGtWA1uM91uusNoEdBV0+3Lf/; 18, 25 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-MimeOLE:Thread-Index:Message-ID:X-ELNK-Trace:X-Originating-IP; 18, 25 -- Received: from [75.61.18.94] (helo=user915fa8f284) 18, 25 -- by elasmtp-junco.atl.sa.earthlink.net with asmtp (Exim 4.34) 18, 25 -- id 1HzDwH-0001BO-Ko; Fri, 15 Jun 2007 11:42:17 -0400 18, 25 -- From: "Tony Havics" {ph2-at-sprynet.com} 18, 25 -- To: "Micrscopy Listserve" {microscopy-at-microscopy.com} 18, 25 -- Subject: Inter/Micro 2007 18, 25 -- Date: Fri, 15 Jun 2007 11:42:15 -0400 18, 25 -- MIME-Version: 1.0 18, 25 -- Content-Type: text/plain; 18, 25 -- charset="US-ASCII" 18, 25 -- Content-Transfer-Encoding: 7bit 18, 25 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 18, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 18, 25 -- Thread-Index: AcevY7+canxfNHe0RAukF4ZByfBm0A== 18, 25 -- Message-ID: {E1HzDwH-0001BO-Ko-at-elasmtp-junco.atl.sa.earthlink.net} 18, 25 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f967630eb5f2bc75373f5134b39e4fcc3e350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 18, 25 -- X-Originating-IP: 75.61.18.94 ==============================End of - Headers==============================
Yes... I should have amplified about the UPS units.
Disregarding outages (that's what the UPS are for), spikes and sags are bad and can have catastrophic effects on the equipment (SEM, TEM, PCs, etc.).
There are basically two types of UPS. One is a simple APC brand style that switches on an inverter when the input power fails. These units can include spike clamps but do not handle sags. The second type is double conversion. This is the best method. This type takes input AC and converts it to DC (usually around 240VDC) and inverts it to AC via sine wave driver and LARGE output transformer. This produces pure sine wave output voltage with about 3% stability and total protection from sags and spikes.
If the input power fails, the unit instantly switches from the rectified input AC--} DC to the battery bank. Perfect. Plus, the Liebert has redundancy options. In my Amray days, I used a pair of Toshiba 1400xl+ dual conversion UPS. They worked well but did not have redundancy. The problem with this is that when one failed, the whole system went down. US service support for the Toshiba units was bad. The Liebert units are US made and US supported. After almost two years of continuous operation, neither of the Lieberts has failed. For about $1K, extended warranty is available. This covers the electronics and the batteries. Each battery is actually a module itself and if the monitors think a battery is bad, a light comes on on the battery module and a message is posted about it.
Getting a good UPS at install is the way to go. For a fraction of the cost of the SEM or TEM and accessories, it is cheap insurance.
gary g.
At 05:41 AM 6/15/2007, you wrote:
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==============================Original Headers============================== 13, 20 -- From gary-at-gaugler.com Fri Jun 15 11:20:12 2007 13, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l5FGKBYO012572 13, 20 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jun 2007 11:20:11 -0500 13, 20 -- Message-Id: {200706151620.l5FGKBYO012572-at-ns.microscopy.com} 13, 20 -- Received: (qmail 19581 invoked from network); 15 Jun 2007 09:20:11 -0700 13, 20 -- Received: by simscan 1.1.0 ppid: 19578, pid: 19579, t: 0.1280s 13, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 13, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 13, 20 -- by qsmtp1 with SMTP; 15 Jun 2007 09:20:11 -0700 13, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 20 -- Date: Fri, 15 Jun 2007 09:20:07 -0800 13, 20 -- To: paul_hazelton-at-umanitoba.ca 13, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 13, 20 -- Subject: Re: [Microscopy] UPS for TEMs 13, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 13, 20 -- In-Reply-To: {200706151341.l5FDflDM030494-at-ns.microscopy.com} 13, 20 -- References: {200706151341.l5FDflDM030494-at-ns.microscopy.com} 13, 20 -- Mime-Version: 1.0 13, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-1CCC3885 ==============================End of - Headers==============================
On Jun 14, 2007, at 5:59 PM, drk-at-SHCC.org wrote:
} We are renovating our lab to install an FEI Tecnai G2 20TEM. As we } think } about the electrical needs, we wonder about the necessity of backing } the } system with an uninterrupted power supply and generator. My } understanding } is that in the event of a power outage, the system will shut down, the } pneumatic valves will close and the microscope will survive. But I } wonder } about the support computers. I am asking for the advice of others in } how } they have addressed UPS for their modern TEM systems. } Dear Doug, We have a T12 and a TF30H, and we only have a UPS on the 30. We have not had any power failures that affected the computers, but we did have a failure in the UPS (fortunately, no power failures during the several months it took to get the UPS repair booked and done). Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
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Title-Subject: [Filtered] SPM, AFM, STM In Undergraduate Education
Question: On June 26, 2007, Agilent Technologies will be hosting a free web-based seminar on the Integration of AFM in Undergraduate Education. Jayne Garno, an assistant professor of chemistry at Louisiana State University, is working to integrate scanning probe experiments into junior and senior undergraduate laboratory courses in analytical and physical chemistry to give students hands-on experience with molecular imaging. In this informative eSeminar, she will describe her progress. Following Garno's presentation, Dr. Song Xu, an applications scientist with Agilent Technologies, will discuss research-grade AFM instrumentation appropriate for use in undergraduate education. There will also be a Q&A session in which all online attendees are welcome to query the presenters. More information, and to register, can be found at http://nano.tm.agilent.com
I didn't get a copy of my reply in the in-box (you obviously did), so it's also attached to the bottom here. Essentially the NA iris works like the iris on the condenser and a camera (to the eye, I don't know about the optical physics).
Using a camera with a macro lens, stopping [closing] the camera iris down to F22 brings everything in the macro shot into focus (a big depth of field), but at the expense of image brightness and probably a little resolution - so you need a longer exposure time. You aren't actually moving the camera any closer to the specimen though, so the 'working distance' hasn't changed. Plus your point of focus is still in the same place (as the lenses/camera haven't moved). See http://www.cs.mtu.edu/~shene/DigiCam/User-Guide/950/depth-of-field.html.
Stopping the NA iris in the high mag oil objective down (to low NA) increases the contrast and probably the depth of field around the focus point, but no doubt at the expense of absolute resolution (hence the lower NA). Naturally high resolution is pig all use without any contrast though to actually see the specimen, hence the advantage of the iris. It probably won't affect the maximum working distance at all though (you are still under oil), other than perhaps a greater depth of focus around the area you are focussed on (you can't physically move the objective into the sample any more). To increase actual working distance you have move the internal lenses (or one anyway) up and down, using a Working Distance [WD] collar if fitted (I've never seen a variable NA iris and variable WD lens collar fitted together on one objective though).
The increased contrast and greater depth of field at the lower NA iris setting will bring out intra-cellular structures far better, and it works with DIC as well. We use a Leica 63x [1.4-0.6 variable NA] HCX PL APO oil objective, which cost around £5,000 I believe. Partly due to it being a flash well made expensive objective, this Leica 'blue' variable NA 63x oil objective's image quality will always be far better than that obtained our long working distance Olympus LCPLFL 60x air objective [NA 0.7], the image quality of which is relatively appalling - but of course it can see well into the gels we use, unlike the Leica high NA oil version, and so is our only choice for such things. The Olympus 60x also has a variable NA to adjust the focus depth and contrast around the point of focus, but image quality at either NA extreme is still quite poor compared to the Leica oil (but it has that long working distance and can be physically moved to focus far further into the specimen).
Well that’s how I see it anyway (and it must be true as another guy at a conference I was at said a similar thing to me once). Unfortunately I don't have access to these objectives any more, as I would like to investigate it further (that’s the fun of this list-server it generates interesting questions).
We generally use these variable NA 63x objectives with fluorescent samples and z-slices under a laser confocal, so I doubt we'd notice any depth of field improvements with the lower NA setting as we are sticking the centre of the focus anyway.
Regards
Keith
----------------------- Dr Keith J Morris [Formerly] Imaging Facilities Manager Cell Biology Institute of Ophthalmology UCL, London EC1V 9EL
Numerical Aperture: This is a critical value that indicates the light acceptance angle, which in turn determines the light gathering power, the resolving power, and depth of field of the objective.
Thus the higher the resolving power (NA) of an objective, the lower the depth of field (and so working distance). So a high NA objective may not suite all applications. Indeed a typical high magnification objective focus point will only just penetrate through a standard 0.17mm glass coverslip. An air objective will have a far greater (relatively) depth of field and thus a far longer working distance, particularly an LD 'long working distance' variety - but the image quality will be noticeably poorer (not a problem though if you can't see squat with your high NA short working distance oil immersion lens). This is all to do with airey disks and stuff, [and also a lot to do with the price of the objective and how well it's made]. Image contrast, as well as resolution, is also an important consideration here.
'Working distance' correction Collar
An adjustment [correction] collar for cover glass thickness (not NA) is sometimes provided on high-NA microscope objective lenses. Rotation of the collar adjusts the height of certain lens elements in the objective lens to compensate for variations in coverslip thickness or immersion media. Thus it provides an adjustable working distance. At high NAs, even a small deviation of the coverslip thickness (by as little as a few micrometers in some cases), or refractive index of the immersion medium from the designated standard, can introduce significant aberrations.
Variable NA collar
Other objectives specifically designed for transmitted light fluorescence and darkfield imaging are sometimes equipped with an internal iris diaphragm that allows for adjustment of the effective numerical aperture [NA]. A 60x apochromat objective can have a numerical aperture of 1.4, one of the highest attainable in modern microscopes using immersion oil as an imaging medium.
Variable Numerical Aperture Objectives: Specimens with unusually high fluorescence quantum yields and/or very bright darkfield specimens often induce image flare by light emitted from areas outside the focal plane. To compensate for this artefact, manufacturers offer high numerical aperture objectives that are equipped with an internal iris diaphragm to increase image contrast during photomicrography or digital imaging. Opening or closing the iris diaphragm determines the size of the objective rear aperture yielding a variable numerical aperture range between 0.5 and the objective's upper limit (up to 1.35-1.4 with apochromatic objectives). Although iris diaphragms were once utilized in a wide variety of objective designs, modern variable numerical aperture objectives are usually at the high end (60x to 150x) of the magnification range.
A variable NA collar on an oil immersion thus help with contrast enhancement when set to low NA, but it's not going to help it in any way achieve the long working distance of a dry LD objective of the same magnification. I must admit that I don't bother too much with the physics equations when looking down the microscope, as any decent microscope rep should be able provide you with a set of objectives to assess which one suites your needs best before you buy. Typically you would go for the highest NA you can for the given working distance required. Manufacturer's websites provide the objectives working distance (in mm) and NA info. Modern ultra-high NA objectives are required for TIRF applications and hi-res DIC contrast enhancement.
To achieve higher NA than 0.95 for objectives they have to be used with immersion media between front lens and specimen. Oil or water is mostly used for that purpose. Because objectives have to be specially designed for this, the type of immersion media is always mentioned on the objective like on the UPlanFLN 60x/1.25 Oil Iris objective. This objective needs oil as an immersion media and can not produce good image quality without it.
High NA objectives also collect more light and if you compare two objectives with fluorescent samples, the high NA objective should produce a noticeably brighter (and preferable) image. Again though the high NA objective is generally far more expensive and it's better attention to quality optics/coatings will also be factor in it's superior performance. An objective also needs to be PLAN Apochromat and fluorescence friendly for quality fluorophore imaging.
The only problem with using our variable NA collar objectives here is that we use inverted optics and have no idea where the NA collar is actually set [e.g. if it's moved accidentally or the previous user has adjusted it]. I do put a chart on the wall telling users which way to turn the NA collar for highest NA (viewed from above), but I'm not sure all users bother with this. I have noticed slight changes in um/pixel calibration (less than 10% difference, and it's linear) between the two NA extremes on some objectives.
To be told more than you probably want to know about resolving power and NA see:
----------------------- Dr Keith J Morris [Formerly] Imaging Facilities Manager Cell Biology Institute of Ophthalmology UCL, London EC1V 9EL
-----Original Message----- X-from: david.knecht-at-uconn.edu [mailto:david.knecht-at-uconn.edu] Sent: 15 June 2007 15:25 To: keith.morris-at-ucl.ac.uk
Lots of useful information in the responses, but let me clarify the question. A colleague is using his stopped down 40x oil objective in order to visualize vesicles throughout the volume of the cell in one image rather than confocal sectioning. I am trying to understand why it works and whether it would work equivalently with a dry objective. Obviously the light cone from a 1.4 NA 40x oil objective (whether iris is closed or not) and a 40x long working distance dry objective are different. The depth of field varies with numerical aperture, so a 40x 1.4 oil immersion lens, should inherently have a shallower depth of focus. Therefore, it makes no intuitive sense to me that you would #1-increase the depth of focus, and #2- get the same results with a 40x oil 1.4 in which you stop down the iris to . 5NA, as a .5NA LWD dry lens. The excitation spot profile of the 40x oil objective is not going to enlarge as you stop down the iris with the same input light path. So I am trying to understand how the focal depth/resolution/xyz profile of the excitation/emission etc. is for fluorescence in comparing those two situations. Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
On Jun 15, 2007, at 9:19 AM, keith.morris-at-ucl.ac.uk wrote:
==============================Original Headers============================== 48, 25 -- From keith.morris-at-ucl.ac.uk Sat Jun 16 08:19:51 2007 48, 25 -- Received: from smtp3.global.net.uk (smtp3.global.net.uk [80.189.92.91]) 48, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l5GDJoXt030808 48, 25 -- for {microscopy-at-microscopy.com} ; Sat, 16 Jun 2007 08:19:51 -0500 48, 25 -- Received: from 9.211.125.91.gr7.adsl.brightview.com ([91.125.211.9] helo=loungepc) 48, 25 -- by smtp3.global.net.uk with esmtp (Exim 4.66 (FreeBSD)) 48, 25 -- (envelope-from {keith.morris-at-ucl.ac.uk} ) 48, 25 -- id 1HzYBx-0009QA-GU 48, 25 -- for microscopy-at-microscopy.com; Sat, 16 Jun 2007 14:19:49 +0100 48, 25 -- From: "Keith Morris" {keith.morris-at-ucl.ac.uk} 48, 25 -- To: {microscopy-at-microscopy.com} 48, 25 -- References: {200706151424.l5FEOk8D025606-at-ns.microscopy.com} 48, 25 -- Subject: RE: [Microscopy] objective NA 48, 25 -- Date: Mon, 18 Jun 2007 14:19:52 +0100 48, 25 -- Message-ID: {000901c7b1ab$5b21e360$0501a8c0-at-loungepc} 48, 25 -- MIME-Version: 1.0 48, 25 -- Content-Type: text/plain; 48, 25 -- charset="iso-8859-1" 48, 25 -- X-Mailer: Microsoft Office Outlook 11 48, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 48, 25 -- Thread-Index: AcevWPLeAhOLNmY0SK6xlsnfsVMErQCSN70g 48, 25 -- In-Reply-To: {200706151424.l5FEOk8D025606-at-ns.microscopy.com} 48, 25 -- Authenticated-Sender: 48, 25 -- Content-Transfer-Encoding: 8bit 48, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l5GDJoXt030808 ==============================End of - Headers==============================
OK, I've been looking around for a group that talks/works on/etc. older microscopes. But I have not been successful in finding such a group.
I have just picked up an old Olympus PMG metallograph. It is an absolutely fascinating piece of optical equipment. I've never seen one like it. Anyway, as one of my (ever increasing number of) extra-curricula activities, I've decided I'd like to either restore this instrument, or alter it so that I can actually use it as a modern metallograph with digital