Don't understand the response below. Everything I received on this topic came through the Listserver plus it seems that the original request for info was also to the Listserver and that responses are of interest to everyone.
Ted
----- Original Message ---- X-from: Jim Quinn www.matscieng.sunysb.edu} To: drteddunne-at-yahoo.com Sent: Saturday, June 30, 2007 11:52:50 PM
I heartily agree! Make them suffer!)
-----Original Message-----
Except that students in my microprobe course who try to use these terms interchangeably loose points. The same happens in the TEM course taught at our university. It is the same difference between potential and potential energy -- subtle but non-trivial.
Best, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu Personal Website: http://umn.edu/~frah0010 ==========================
==============================Original Headers============================== 7, 25 -- From Philip.Koeck-at-biosci.ki.se Mon Jul 2 02:39:43 2007 7, 25 -- Received: from smtp.biosci.ki.se (smtp.biosci.ki.se [130.237.109.196]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l627dg1d003720 7, 25 -- for {microscopy-at-microscopy.com} ; Mon, 2 Jul 2007 02:39:43 -0500 7, 25 -- Received: from smtp.biosci.ki.se (localhost [127.0.0.1]) 7, 25 -- by localhost (Postfix) with ESMTP id 374E34FF08; 7, 25 -- Mon, 2 Jul 2007 09:39:42 +0200 (CEST) 7, 25 -- Received: from whale.csb.ki.se (whale.csb.ki.se [130.237.109.8]) 7, 25 -- by smtp.biosci.ki.se (Postfix) with SMTP id 1F3CB4FF07; 7, 25 -- Mon, 2 Jul 2007 09:39:42 +0200 (CEST) 7, 25 -- Received: from rapana.csb.ki.se by whale.csb.ki.se (5.65v4.0/1.1.10.5/03Feb98-0237PM) 7, 25 -- id AA23029; Mon, 2 Jul 2007 09:39:41 +0200 7, 25 -- Message-Id: {10707020739.AA23029-at-whale.csb.ki.se} 7, 25 -- From: "Philip Koeck" {Philip.Koeck-at-biosci.ki.se} 7, 25 -- To: {frah0010-at-umn.edu} , {microscopy-at-microscopy.com} 7, 25 -- Subject: RE: [Microscopy] kV or keV? 7, 25 -- Date: Mon, 2 Jul 2007 09:40:35 +0200 7, 25 -- Mime-Version: 1.0 7, 25 -- Content-Type: text/plain; 7, 25 -- charset="us-ascii" 7, 25 -- Content-Transfer-Encoding: 7bit 7, 25 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353 7, 25 -- X-Mimeole: Produced By Microsoft MimeOLE V6.00.2900.3138 7, 25 -- Thread-Index: Ace6SHxZa1jehf4NS1u3yKUO3Il0lwCM3eSw 7, 25 -- In-Reply-To: {200706291224.l5TCOmZS002527-at-ns.microscopy.com} ==============================End of - Headers==============================
I would loose much points in your courses !! Is it necessary to be so rigorous ?
As soon as one put an electrostatic element in an electron optical device, one have to handel with eV. An electrostatic lens changes the kinetcic energy of an electron, and SEM and TEM have much such elements (wehnelt, extraction (FE) and accelerating anodes, SE or BE detectors, electron filtering).
From a purist point of view, it could be said that only the electronic engeneer is concerned with the kV notation, in the design/mainteance of the HV circuit. The user has more to deal with the kinetic energy.
In the practise, it seems that biologists and mineralogist use more kV, and physicists use more keV. None is wrong !
So be kind with your studens, there is much enough stuff which is difficult to understand !
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Our imaging core facility needs to have rules of how to handle viral- infected (replication competent) specimen in the microscopy room. I cannot simply forbid such samples as people need virus infection in order to efficiently express fluorescent proteins.
In the virus core facility, Barrydin (cocospropylendiaminguanidindiacetat, didecyloxyethylmethylammoniumpropionat; Pan Biotech) is used for disinfection. Does anybody know if this agent can safely be used for cleaning microscopes in case of a spill? How about objectives? As both retro/lenti- and adenoviruses are being used, ethanol is not enough.
Perhaps somebody has already devised guidelines for handling infectious microscope samples and is willing to share them?
Regards, Anne -- Anne Vaahtokari, Ph.D Imaging Coordinator Molecular Imaging Unit Biomedicum Helsinki P.O. Box 63 (Haartmaninkatu 8) FIN-00014 University of Helsinki Finland Tel: +358-9-191 25579 Fax: +358-9-191 25554 E-mail: anne.vaahtokari-at-helsinki.fi MIU web site: www.miu.helsinki.fi
==============================Original Headers============================== 8, 28 -- From vaahtoka-at-mappi.helsinki.fi Mon Jul 2 07:16:15 2007 8, 28 -- Received: from sender-02.it.helsinki.fi (sender-02.it.helsinki.fi [128.214.205.137]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l62CGE7F032716 8, 28 -- for {microscopy-at-microscopy.com} ; Mon, 2 Jul 2007 07:16:14 -0500 8, 28 -- Received: from quas.it.helsinki.fi (quas.it.helsinki.fi [128.214.205.29]) 8, 28 -- by sender-02.it.helsinki.fi (8.13.8/8.13.8) with ESMTP id l62CGC5d024362 8, 28 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 28 -- for {microscopy-at-microscopy.com} ; Mon, 2 Jul 2007 15:16:12 +0300 8, 28 -- Received: from quas.it.helsinki.fi (localhost.localdomain [127.0.0.1]) 8, 28 -- by quas.it.helsinki.fi (8.13.1/8.13.1) with ESMTP id l62CGCO2003027 8, 28 -- for {microscopy-at-microscopy.com} ; Mon, 2 Jul 2007 15:16:12 +0300 8, 28 -- Received: (from apache-at-localhost) 8, 28 -- by quas.it.helsinki.fi (8.13.1/8.13.1/Submit) id l62CGCmC003025 8, 28 -- for microscopy-at-microscopy.com; Mon, 2 Jul 2007 15:16:12 +0300 8, 28 -- Received: from 128.214.99.40 ( [128.214.99.40]) 8, 28 -- as user vaahtoka-at-localhost by www1.helsinki.fi with HTTP; 8, 28 -- Mon, 2 Jul 2007 15:16:12 +0300 8, 28 -- Message-ID: {1183378572.4688ec8c5da14-at-www1.helsinki.fi} 8, 28 -- Date: Mon, 2 Jul 2007 15:16:12 +0300 8, 28 -- From: Anne Vaahtokari {anne.vaahtokari-at-helsinki.fi} 8, 28 -- To: microscopy-at-microscopy.com 8, 28 -- Subject: Virus infected microscope samples 8, 28 -- MIME-Version: 1.0 8, 28 -- Content-Type: text/plain; charset=us-ascii 8, 28 -- Content-Transfer-Encoding: 7bit 8, 28 -- User-Agent: Internet Messaging Program (IMP) 3.1 8, 28 -- X-Originating-IP: 128.214.99.40 8, 28 -- Sender: vaahtoka-at-mappi.helsinki.fi ==============================End of - Headers==============================
I suppose you are talking about light microscopes. I can't answer directly to your question, but I would definitively avoid any contact with the objectives, it would make things a lot easier to handle. As far as possible use coverslips and desinfect them carefully before microscoping. Handling replication-competent human retroviruses otherwise (outside of a proper hood) is unconsciousness for me. And, if not stored, the samples must be autoclaved afterwards of course.
Gluraldehydes and formaldehydes are also desinfecting agents, I don't think they could damade objectives but they need hours to work efficiently and I am not sure that soaking objectives for hours in aqueous solutions is very good.
Now an alternative to chemical decontamination is the possibility to UV-B decontaminate. I think UV-B kill also virus.
Probably the best partner to discuss the sentivity of the objectives to various chemicals is the constructor.
Best regards,
Stephane
--- anne.vaahtokari-at-helsinki.fi wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello All, } } Our imaging core facility needs to have rules of how } to handle viral- } infected (replication competent) specimen in the } microscopy room. I cannot } simply forbid such samples as people need virus } infection in order to } efficiently express fluorescent proteins. } } In the virus core facility, Barrydin } (cocospropylendiaminguanidindiacetat, } didecyloxyethylmethylammoniumpropionat; Pan Biotech) } is used for } disinfection. Does anybody know if this agent can } safely be used for } cleaning microscopes in case of a spill? How about } objectives? As both } retro/lenti- and adenoviruses are being used, } ethanol is not enough. } } Perhaps somebody has already devised guidelines for } handling infectious } microscope samples and is willing to share them? } } Regards, } Anne } -- } Anne Vaahtokari, Ph.D } Imaging Coordinator } Molecular Imaging Unit } Biomedicum Helsinki } P.O. Box 63 (Haartmaninkatu 8) } FIN-00014 University of Helsinki } Finland } Tel: +358-9-191 25579 } Fax: +358-9-191 25554 } E-mail: anne.vaahtokari-at-helsinki.fi } MIU web site: www.miu.helsinki.fi } } } } } ==============================Original } Headers============================== } 8, 28 -- From vaahtoka-at-mappi.helsinki.fi Mon Jul 2 } 07:16:15 2007 } 8, 28 -- Received: from sender-02.it.helsinki.fi } (sender-02.it.helsinki.fi [128.214.205.137]) } 8, 28 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l62CGE7F032716 } 8, 28 -- for {microscopy-at-microscopy.com} ; Mon, 2 } Jul 2007 07:16:14 -0500 } 8, 28 -- Received: from quas.it.helsinki.fi } (quas.it.helsinki.fi [128.214.205.29]) } 8, 28 -- by sender-02.it.helsinki.fi } (8.13.8/8.13.8) with ESMTP id l62CGC5d024362 } 8, 28 -- (version=TLSv1/SSLv3 } cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } 8, 28 -- for {microscopy-at-microscopy.com} ; Mon, 2 } Jul 2007 15:16:12 +0300 } 8, 28 -- Received: from quas.it.helsinki.fi } (localhost.localdomain [127.0.0.1]) } 8, 28 -- by quas.it.helsinki.fi (8.13.1/8.13.1) } with ESMTP id l62CGCO2003027 } 8, 28 -- for {microscopy-at-microscopy.com} ; Mon, 2 } Jul 2007 15:16:12 +0300 } 8, 28 -- Received: (from apache-at-localhost) } 8, 28 -- by quas.it.helsinki.fi } (8.13.1/8.13.1/Submit) id l62CGCmC003025 } 8, 28 -- for microscopy-at-microscopy.com; Mon, 2 Jul } 2007 15:16:12 +0300 } 8, 28 -- Received: from 128.214.99.40 ( } [128.214.99.40]) } 8, 28 -- as user vaahtoka-at-localhost by } www1.helsinki.fi with HTTP; } 8, 28 -- Mon, 2 Jul 2007 15:16:12 +0300 } 8, 28 -- Message-ID: } {1183378572.4688ec8c5da14-at-www1.helsinki.fi} } 8, 28 -- Date: Mon, 2 Jul 2007 15:16:12 +0300 } 8, 28 -- From: Anne Vaahtokari } {anne.vaahtokari-at-helsinki.fi} } 8, 28 -- To: microscopy-at-microscopy.com } 8, 28 -- Subject: Virus infected microscope samples } 8, 28 -- MIME-Version: 1.0 } 8, 28 -- Content-Type: text/plain; charset=us-ascii } 8, 28 -- Content-Transfer-Encoding: 7bit } 8, 28 -- User-Agent: Internet Messaging Program } (IMP) 3.1 } 8, 28 -- X-Originating-IP: 128.214.99.40 } 8, 28 -- Sender: vaahtoka-at-mappi.helsinki.fi } ==============================End of - } Headers============================== }
____________________________________________________________________________________ Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. http://answers.yahoo.com/dir/?link=list&sid=396545469
} I would loose much points in your courses!! Is it necessary to be } so rigorous? ... So be kind with your studens, there is much enough } stuff which is difficult to understand!
Jacques, Philip, and everyone,
I do not grade such details just to be nasty or make the students suffer (although they might disagree). Many of these students will go on to use our electron microprobe for their research, and they will be presenting posters, giving conference talks, and submitting papers to publish. If one of the students mentions using "an accelerating voltage of 15 keV" or observing "X-ray peaks at 4.57 and 7.14 kV," there will be reviewers or audience members who notice such mistakes and may start to doubt the student's understanding of the analytical technique and, consequently, their results and conclusions. I grade for such details so that the students to do make the same mistakes later and embarrass themselves (and me as their teacher) when it really matters. Hopefully they understand that my rigorous grading is in their best interests -- I think that most of them do, and I hear far more grumbling about the essays that I make them write on quizzes and exams.
Best, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu Personal Website: http://umn.edu/~frah0010
A correction and clarification for my posting: the virus infected samples are NOT replication competent, and they are for live cell imaging (both wide-field and confocal microscopes are used).
Anne -- Anne Vaahtokari, Ph.D Imaging Coordinator Molecular Imaging Unit Biomedicum Helsinki P.O. Box 63 (Haartmaninkatu 8) FIN-00014 University of Helsinki Finland Tel: +358-9-191 25579 Fax: +358-9-191 25554 E-mail: anne.vaahtokari-at-helsinki.fi MIU web site: www.miu.helsinki.fi
==============================Original Headers============================== 6, 28 -- From vaahtoka-at-mappi.helsinki.fi Mon Jul 2 08:38:27 2007 6, 28 -- Received: from sender-03.it.helsinki.fi (sender-03.it.helsinki.fi [128.214.205.141]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l62DcQKM005170 6, 28 -- for {microscopy-at-microscopy.com} ; Mon, 2 Jul 2007 08:38:27 -0500 6, 28 -- Received: from quas.it.helsinki.fi (quas.it.helsinki.fi [128.214.205.29]) 6, 28 -- by sender-03.it.helsinki.fi (8.13.1/8.13.1) with ESMTP id l62DcPWX029287 6, 28 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 6, 28 -- for {microscopy-at-microscopy.com} ; Mon, 2 Jul 2007 16:38:25 +0300 6, 28 -- Received: from quas.it.helsinki.fi (localhost.localdomain [127.0.0.1]) 6, 28 -- by quas.it.helsinki.fi (8.13.1/8.13.1) with ESMTP id l62DcPRA021000 6, 28 -- for {microscopy-at-microscopy.com} ; Mon, 2 Jul 2007 16:38:25 +0300 6, 28 -- Received: (from apache-at-localhost) 6, 28 -- by quas.it.helsinki.fi (8.13.1/8.13.1/Submit) id l62DcPnO020998 6, 28 -- for microscopy-at-microscopy.com; Mon, 2 Jul 2007 16:38:25 +0300 6, 28 -- Received: from 128.214.99.40 ( [128.214.99.40]) 6, 28 -- as user vaahtoka-at-localhost by www1.helsinki.fi with HTTP; 6, 28 -- Mon, 2 Jul 2007 16:38:25 +0300 6, 28 -- Message-ID: {1183383505.4688ffd12043d-at-www1.helsinki.fi} 6, 28 -- Date: Mon, 2 Jul 2007 16:38:25 +0300 6, 28 -- From: Anne Vaahtokari {anne.vaahtokari-at-helsinki.fi} 6, 28 -- To: microscopy-at-microscopy.com 6, 28 -- Subject: Re: [Microscopy] Virus infected microscope samples 6, 28 -- MIME-Version: 1.0 6, 28 -- Content-Type: text/plain; charset=us-ascii 6, 28 -- Content-Transfer-Encoding: 7bit 6, 28 -- User-Agent: Internet Messaging Program (IMP) 3.1 6, 28 -- X-Originating-IP: 128.214.99.40 6, 28 -- Sender: vaahtoka-at-mappi.helsinki.fi ==============================End of - Headers==============================
Of coarse, I agree that students have to learn the right words/units to express the right concepts. But what I wanted to point out, is that, in the description of the SEM/TEM working principle, the two concepts have their place.
I understand than one accept, in the following sentances, either :
"We have observed sample xyz with a an accelerating voltage of 15 kV" or : "We have observed sample xyz with a an electron beam energy set to 15 keV"
and not the contrary or a unprecise mixing of the two formulations. But one cannot reject the formulation using the energy concept. I don't speak here from x-rays, only from the electrons in the microscope.
In my former mail I mentionned the electrostatic elements of the microscope. In the electron/matter interaction too, it's the energy aspect which plays a role, not the voltage difference used to accelerate/decelerate these electrons. In diffraction, the equivalence is between wavelength and energy.
Jacques
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Ellery Frahm a écrit : } Philip Koeck wrote: } } } I heartily agree! Make them suffer!) } } Jacques Faerber wrote: } } } I would loose much points in your courses!! Is it necessary to be so } } rigorous? ... So be kind with your studens, there is much enough } } stuff which is difficult to understand! } } } Jacques, Philip, and everyone, } } I do not grade such details just to be nasty or make the students } suffer (although they might disagree). Many of these students will go } on to use our electron microprobe for their research, and they will be } presenting posters, giving conference talks, and submitting papers to } publish. If one of the students mentions using "an accelerating } voltage of 15 keV" or observing "X-ray peaks at 4.57 and 7.14 kV," } there will be reviewers or audience members who notice such mistakes } and may start to doubt the student's understanding of the analytical } technique and, consequently, their results and conclusions. I grade } for such details so that the students to do make the same mistakes } later and embarrass themselves (and me as their teacher) when it } really matters. Hopefully they understand that my rigorous grading is } in their best interests -- I think that most of them do, and I hear } far more grumbling about the essays that I make them write on quizzes } and exams. } } Best, } Ellery } } -------------------- } Ellery E. Frahm } Research Fellow & Manager } Electron Microprobe Laboratory } University of Minnesota - Twin Cities } Department of Geology & Geophysics } Lab Website: http://probelab.geo.umn.edu } Personal Website: http://umn.edu/~frah0010
==============================Original Headers============================== 12, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Jul 3 02:52:35 2007 12, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.155]) 12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l637qZrp015043 12, 29 -- for {microscopy-at-microscopy.com} ; Tue, 3 Jul 2007 02:52:35 -0500 12, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 12, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id l637qX4x030560 12, 29 -- ; Tue, 3 Jul 2007 09:52:33 +0200 (CEST) 12, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 12, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id E57841037EAF; 12, 29 -- Tue, 3 Jul 2007 09:51:52 +0200 (CEST) 12, 29 -- Message-ID: {468A001F.2040404-at-ipcms.u-strasbg.fr} 12, 29 -- Date: Tue, 03 Jul 2007 09:51:59 +0200 12, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 12, 29 -- User-Agent: Thunderbird 1.5.0.12 (X11/20070604) 12, 29 -- MIME-Version: 1.0 12, 29 -- To: Ellery Frahm {frah0010-at-umn.edu} , Microscopy-at-microscopy.com 12, 29 -- Subject: Re: [Microscopy] RE: kV or keV? 12, 29 -- References: {200707020747.l627lmVV012877-at-ns.microscopy.com} {4FDB017F-1557-43CB-8E0E-64C7E4A36319-at-umn.edu} 12, 29 -- In-Reply-To: {4FDB017F-1557-43CB-8E0E-64C7E4A36319-at-umn.edu} 12, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 29 -- Content-Transfer-Encoding: 8bit 12, 29 -- X-IPCMS-MailScanner: Found to be clean 12, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 12, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.155]); Tue, 03 Jul 2007 09:52:33 +0200 (CEST) 12, 29 -- X-Virus-Scanned: ClamAV 0.88.7/3583/Tue Jul 3 08:37:57 2007 on mr5.u-strasbg.fr 12, 29 -- X-Virus-Status: Clean 12, 29 -- X-Spam-Status: No, score=-0.1 required=5.0 tests=AWL autolearn=disabled 12, 29 -- version=3.1.8 12, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr5.u-strasbg.fr ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both catherine.bougerol-at-cea.fr as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] SEM of particles on quartz
Question: I have a sample consisting of metallic particles (about 15nm diameter) deposited on a quartz or glass slide. I wonder if and how it is possible to get an image by SEM, with a FEG microscope.
Thanks for the infos! It looks very much like a revolution to me! (if it really works as it is presented ;-)) One can expected a deep penetration of the beam into cells (but a larger dispersion too of course).
The only drawback I noticed: in the FAQ, they do not answer to their own question "What is the price of the QX-202C capsules?" and this is usually bad sign :-D
Regards
Stephane
--- kraftpiano-at-gmail.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I was recently perusing one of the supply catalogs } for SEM supplies, } and I found a wet-cell capsule that has an "Electron } transparent and } vacuum tight window." } (http://www.emsdiasum.com/microscopy/products/sem/wet/technical.aspx) } I was just wondering if anybody has used these, and } what kind of } results they are getting. } } The second part of my question is a bit more } abstract. What quality } makes something electron transparent, and what } materials are electron } transparent? } } --Justin A. Kraft } } ==============================Original } Headers============================== } 3, 26 -- From kraftpiano-at-gmail.com Thu Jun 28 } 09:50:21 2007 } 3, 26 -- Received: from nz-out-0506.google.com } (nz-out-0506.google.com [64.233.162.224]) } 3, 26 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l5SEoLV7028166 } 3, 26 -- for {microscopy-at-microscopy.com} ; Thu, 28 } Jun 2007 09:50:21 -0500 } 3, 26 -- Received: by nz-out-0506.google.com with
Oh, yes, I absolutely agree that both units/concepts (potential/ voltage and energy) have their place in electron microscopy, just as energy and wavelength are both valid ways to think about X-ray analysis -- I didn't mean to suggest otherwise. I simply meant that students must use the proper terminology and units together and in the most appropriate situations, and we agree on that too. Like I said in the previous message, what is unacceptable is, for example, saying "an accelerating voltage of 15 keV" or describing X-ray peaks in units of kV. But students should be familiar with both ways of thinking about the technique.
Best, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu Personal Website: http://umn.edu/~frah0010
On Jul 3, 2007, at 3:00 AM, jacques.faerber-at-ipcms.u-strasbg.fr wrote:
} Hi Ellery and all } } Of coarse, I agree that students have to learn the right words/ } units to } express the right concepts. But what I wanted to point out, is } that, in } the description of the SEM/TEM working principle, the two concepts } have } their place. } } I understand than one accept, in the following sentances, either : } } "We have observed sample xyz with a an accelerating voltage of } 15 kV" } or : } "We have observed sample xyz with a an electron beam energy } set to } 15 keV" } } and not the contrary or a unprecise mixing of the two formulations. } But } one cannot reject the formulation using the energy concept. I don't } speak here from x-rays, only from the electrons in the microscope. } } In my former mail I mentionned the electrostatic elements of the } microscope. In the electron/matter interaction too, it's the energy } aspect which plays a role, not the voltage difference used to } accelerate/decelerate these electrons. In diffraction, the equivalence } is between wavelength and energy. } } Jacques } } J. Faerber } IPCMS-GSI } (Institut de Physique et Chimie des Matériaux de Strasbourg } Groupe Surface et Interfaces) } 23, rue de Loess ; BP43 } 67034 Strasbourg CEDEX 2 } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)3 88 10 72 48 } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr } } } } Ellery Frahm a écrit : } } Philip Koeck wrote: } } } } } I heartily agree! Make them suffer!) } } } } Jacques Faerber wrote: } } } } } I would loose much points in your courses!! Is it necessary to } } } be so } } } rigorous? ... So be kind with your studens, there is much enough } } } stuff which is difficult to understand! } } } } } } Jacques, Philip, and everyone, } } } } I do not grade such details just to be nasty or make the students } } suffer (although they might disagree). Many of these students } } will go } } on to use our electron microprobe for their research, and they } } will be } } presenting posters, giving conference talks, and submitting papers to } } publish. If one of the students mentions using "an accelerating } } voltage of 15 keV" or observing "X-ray peaks at 4.57 and 7.14 kV," } } there will be reviewers or audience members who notice such mistakes } } and may start to doubt the student's understanding of the analytical } } technique and, consequently, their results and conclusions. I grade } } for such details so that the students to do make the same mistakes } } later and embarrass themselves (and me as their teacher) when it } } really matters. Hopefully they understand that my rigorous } } grading is } } in their best interests -- I think that most of them do, and I hear } } far more grumbling about the essays that I make them write on quizzes } } and exams. } } } } Best, } } Ellery } } } } -------------------- } } Ellery E. Frahm } } Research Fellow & Manager } } Electron Microprobe Laboratory } } University of Minnesota - Twin Cities } } Department of Geology & Geophysics } } Lab Website: http://probelab.geo.umn.edu } } Personal Website: http://umn.edu/~frah0010 } } ==============================Original } Headers============================== } 12, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Jul 3 } 02:52:35 2007 } 12, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u- } strasbg.fr [130.79.200.155]) } 12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l637qZrp015043 } 12, 29 -- for {microscopy-at-microscopy.com} ; Tue, 3 Jul 2007 } 02:52:35 -0500 } 12, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr } [130.79.210.2]) } 12, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) } with ESMTP id l637qX4x030560 } 12, 29 -- ; Tue, 3 Jul 2007 09:52:33 +0200 (CEST) } 12, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr } [130.79.152.3]) } 12, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id E57841037EAF; } 12, 29 -- Tue, 3 Jul 2007 09:51:52 +0200 (CEST) } 12, 29 -- Message-ID: {468A001F.2040404-at-ipcms.u-strasbg.fr} } 12, 29 -- Date: Tue, 03 Jul 2007 09:51:59 +0200 } 12, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} } 12, 29 -- User-Agent: Thunderbird 1.5.0.12 (X11/20070604) } 12, 29 -- MIME-Version: 1.0 } 12, 29 -- To: Ellery Frahm {frah0010-at-umn.edu} , } Microscopy-at-microscopy.com } 12, 29 -- Subject: Re: [Microscopy] RE: kV or keV? } 12, 29 -- References: } {200707020747.l627lmVV012877-at-ns.microscopy.com} } {4FDB017F-1557-43CB-8E0E-64C7E4A36319-at-umn.edu} } 12, 29 -- In-Reply-To: {4FDB017F-1557-43CB-8E0E-64C7E4A36319-at-umn.edu} } 12, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 29 -- Content-Transfer-Encoding: 8bit } 12, 29 -- X-IPCMS-MailScanner: Found to be clean } 12, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr } 12, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter- } greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.155]); Tue, 03 Jul } 2007 09:52:33 +0200 (CEST) } 12, 29 -- X-Virus-Scanned: ClamAV 0.88.7/3583/Tue Jul 3 08:37:57 } 2007 on mr5.u-strasbg.fr } 12, 29 -- X-Virus-Status: Clean } 12, 29 -- X-Spam-Status: No, score=-0.1 required=5.0 tests=AWL } autolearn=disabled } 12, 29 -- version=3.1.8 } 12, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) } on mr5.u-strasbg.fr } ==============================End of - } Headers============================== }
==============================Original Headers============================== 9, 20 -- From frah0010-at-umn.edu Tue Jul 3 08:35:54 2007 9, 20 -- Received: from mta-a2.tc.umn.edu (mta-a2.tc.umn.edu [134.84.119.206]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l63DZrGX025854 9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 3 Jul 2007 08:35:54 -0500 9, 20 -- Received: from [10.0.1.3] (c-75-72-182-252.hsd1.mn.comcast.net [75.72.182.252]) 9, 20 -- by mta-a2.tc.umn.edu (UMN smtpd) with ESMTP 9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 3 Jul 2007 08:35:48 -0500 (CDT) 9, 20 -- X-Umn-Remote-Mta: [N] c-75-72-182-252.hsd1.mn.comcast.net [75.72.182.252] #+TS+AU+HN 9, 20 -- Mime-Version: 1.0 (Apple Message framework v752.2) 9, 20 -- In-Reply-To: {200707030800.l6380WHB024226-at-ns.microscopy.com} 9, 20 -- References: {200707030800.l6380WHB024226-at-ns.microscopy.com} 9, 20 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 9, 20 -- Message-Id: {DB896335-98ED-4EAF-B1FF-6E8A34AEE218-at-umn.edu} 9, 20 -- From: Ellery Frahm {frah0010-at-umn.edu} 9, 20 -- Subject: Re: [Microscopy] kV or keV? 9, 20 -- Date: Tue, 3 Jul 2007 08:35:46 -0500 9, 20 -- To: microscopy-at-microscopy.com 9, 20 -- X-Mailer: Apple Mail (2.752.2) 9, 20 -- Content-Transfer-Encoding: 8bit 9, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l63DZrGX025854 ==============================End of - Headers==============================
I'm posting for a colleague who is interested in finding various vendors of the technology listed below. Obviously, vendors are encouraged to reply. Anybody have vendor/system recommendations? Randy
Who manufactures micro IR and Raman systems (various combinations of NIR, mid IR, far IR, Raman) for our existing light microscopes? It shouldn't necessarily be a system that is mounted right on top of the microscope (as in Smiths detection case). There are bench top systems that can be coupled to a light microscope as well.
----------------------------------------------------------- Jonas Baltrusaitis, Ph.D. Postdoctorate Research Fellow Department of Chemistry and Central Microscopy Research Facility 85 EMRB University of Iowa Iowa City, IA 52242 phone: 319-335-8142
==============================Original Headers============================== 5, 23 -- From randy-nessler-at-uiowa.edu Tue Jul 3 12:44:41 2007 5, 23 -- Received: from hc-smtp1.healthcare.uiowa.edu (hc-smtp1.healthcare.uiowa.edu [129.255.210.32]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l63Hifb4012603 5, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Jul 2007 12:44:41 -0500 5, 23 -- Received: from HC-MAIL11.healthcare.uiowa.edu ([129.255.112.33]) by hc-smtp1.healthcare.uiowa.edu with Microsoft SMTPSVC(6.0.3790.1830); 5, 23 -- Tue, 3 Jul 2007 12:44:40 -0500 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 23 -- Content-class: urn:content-classes:message 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="us-ascii" 5, 23 -- Subject: Micro IR and Ramam systems 5, 23 -- Date: Tue, 3 Jul 2007 12:44:40 -0500 5, 23 -- Message-ID: {CBAD8B8185C05346A5B74AE1E12CBF2A65584C-at-HC-MAIL11.healthcare.uiowa.edu} 5, 23 -- X-MS-Has-Attach: 5, 23 -- X-MS-TNEF-Correlator: 5, 23 -- Thread-Topic: Micro IR and Ramam systems 5, 23 -- Thread-Index: Ace9mdUL829m68CWSJGMJjTEV6xDog== 5, 23 -- From: "Nessler, Randy A" {randy-nessler-at-uiowa.edu} 5, 23 -- To: {Microscopy-at-microscopy.com} 5, 23 -- X-OriginalArrivalTime: 03 Jul 2007 17:44:40.0964 (UTC) FILETIME=[D54DCC40:01C7BD99] 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l63Hifb4012603 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both damondewsy-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: Hello, I'm trying to reassemble a Phillips EM201 TEM. I have the other 3 parts of the manual (including Pre-installation). I was wondering if anyone might be able to tell me who I might get this manual from or if someone might have a pdf copy of it. Thanks.
Damon Dewsbury MSc candidate UofT- Dept. of EEB damondewsy-at-yahoo.com
I'm hoping someone can explain a strange phenomenon that's occurred with my EDAX EDS - JEOL SEM system. Actually, two things: 1) There is a huge 'noise' peak at the far left of the EDS energy spectrum that doesn't correspond to elements present, and 2) The count rate (CPS) while gathering a spectrum is abnormally high, but the dead time (DT%) is very low - this is the opposite of what normally occurs. It happens in both secondary and backscattered electron modes. I'm looking at metal samples, and I gathered a spectrum from the aluminum sample holder to make sure what I was seeing was real. Unfortunately, it is. Any ideas of what's going on, or what troubleshooting I should try, will be greatly appreciated.
Thank you,
Jane L. LaGoy Laboratory Services Manager/ Development Engineer Bodycote North America 155 River Street Andover, MA 01810 978-470-1620 x450 FAX: 978-475-2951 jane.lagoy-at-bodycote.com The only people to get even with are those who have helped you.
This Email and all attachments hereto, if any, are covered by the provisions of the U.S. Electronic Communications Privacy Act. Additionally, all contents of this communication and all attachments hereto shall be deemed to be confidential and the contents thereof are proprietary to Bodycote Thermal Processing, Inc. ("Bodycote"). If this communication has been sent to you in error, please reply to the sender that you received it and then delete the message immediately. Any dissemination, distribution, copying or reproduction of this message other than by its intended recipient is strictly prohibited.
==============================Original Headers============================== 8, 31 -- From Jane.LaGoy-at-Bodycote.com Thu Jul 5 08:46:37 2007 8, 31 -- Received: from mail131.messagelabs.com (mail131.messagelabs.com [216.82.242.99]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l65Dkaob025327 8, 31 -- for {Microscopy-at-MSA.Microscopy.com} ; Thu, 5 Jul 2007 08:46:36 -0500 8, 31 -- X-VirusChecked: Checked 8, 31 -- X-Env-Sender: Jane.LaGoy-at-Bodycote.com 8, 31 -- X-Msg-Ref: server-10.tower-131.messagelabs.com!1183643194!23275600!1 8, 31 -- X-StarScan-Version: 5.5.12.11; banners=-,-,- 8, 31 -- X-Originating-IP: [209.235.7.177] 8, 31 -- Received: (qmail 10263 invoked from network); 5 Jul 2007 13:46:34 -0000 8, 31 -- Received: from 177-209.235.7.appsitehosting.com (HELO VM200EXC02.ad.bodycote.na) (209.235.7.177) 8, 31 -- by server-10.tower-131.messagelabs.com with SMTP; 5 Jul 2007 13:46:34 -0000 8, 31 -- Received: from VM200EXC01.ad.bodycote.na ([10.200.20.9]) by VM200EXC02.ad.bodycote.na with Microsoft SMTPSVC(6.0.3790.3959); 8, 31 -- Thu, 5 Jul 2007 09:46:34 -0400 8, 31 -- Content-Class: urn:content-classes:message 8, 31 -- MIME-Version: 1.0 8, 31 -- Content-Type: text/plain; 8, 31 -- charset="us-ascii" 8, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.3959 8, 31 -- Subject: Problem with EDS signal on SEM 8, 31 -- Date: Thu, 5 Jul 2007 09:46:38 -0400 8, 31 -- Message-ID: {412949C8D462414B9BE7A0921A9C84160C22EF-at-VM200EXC01.ad.bodycote.na} 8, 31 -- X-MS-Has-Attach: 8, 31 -- X-MS-TNEF-Correlator: 8, 31 -- Thread-Topic: Problem with EDS signal on SEM 8, 31 -- thread-index: Ace/CullcuGZLSB7RiKlvXGCKzEJew== 8, 31 -- From: "Jane LaGoy" {Jane.LaGoy-at-Bodycote.com} 8, 31 -- To: {Microscopy-at-MSA.Microscopy.com} 8, 31 -- X-OriginalArrivalTime: 05 Jul 2007 13:46:34.0983 (UTC) FILETIME=[E7059770:01C7BF0A] 8, 31 -- Content-Transfer-Encoding: 8bit 8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l65Dkaob025327 ==============================End of - Headers==============================
A number of possibilities come to mind, assuming you have a UTW, LN2 cooled detector. Imaging mode (SEI vs. BSE) should have no bearing on the EDS noise unless (remote possibility) a detector is generating electrical noise.
1. There could be a problem with the pulse processor. Low-end noise is suppressed when working properly.
2a. LN2 boiling due to ice crystals in dewar.
2b. LN2 boiling due to detector vacuum leak (typically from bad UTW)
3. Extraneous light source. Usually from a vacuum gauge or the LED illumination from a chamber (TV) scope.
5. Damaged detector from warm-up with bias applied.
6. Missing electron trap on end of detector.
7. Ground loop in signal wires. E.G: broken cable shield, disconnected ground wires. (Has the wiring layout changed or any electrically noisy new equipment been installed anywhere nearby?)
...All that comes to mind at the moment. Good Luck!
Woody
NW (Woody) White Jr BWXT Services Lynchburg, VA http://www.bwxt.com/operations/semlab.html
-----Original Message----- X-from: Jane.LaGoy-at-Bodycote.com [mailto:Jane.LaGoy-at-Bodycote.com] Sent: Thursday, July 05, 2007 9:48 AM To: White, Woody N.
Hello microscopists,
I'm hoping someone can explain a strange phenomenon that's occurred with my EDAX EDS - JEOL SEM system. Actually, two things: 1) There is a huge 'noise' peak at the far left of the EDS energy spectrum that doesn't correspond to elements present, and 2) The count rate (CPS) while gathering a spectrum is abnormally high, but the dead time (DT%) is very low - this is the opposite of what normally occurs. It happens in both secondary and backscattered electron modes. I'm looking at metal samples, and I gathered a spectrum from the aluminum sample holder to make sure what I was seeing was real. Unfortunately, it is. Any ideas of what's going on, or what troubleshooting I should try, will be greatly appreciated.
Thank you,
Jane L. LaGoy Laboratory Services Manager/ Development Engineer Bodycote North America 155 River Street Andover, MA 01810 978-470-1620 x450 FAX: 978-475-2951 jane.lagoy-at-bodycote.com The only people to get even with are those who have helped you.
This Email and all attachments hereto, if any, are covered by the provisions of the U.S. Electronic Communications Privacy Act. Additionally, all contents of this communication and all attachments hereto shall be deemed to be confidential and the contents thereof are proprietary to Bodycote Thermal Processing, Inc. ("Bodycote"). If this communication has been sent to you in error, please reply to the sender that you received it and then delete the message immediately. Any dissemination, distribution, copying or reproduction of this message other than by its intended recipient is strictly prohibited.
==============================Original Headers============================== 8, 31 -- From Jane.LaGoy-at-Bodycote.com Thu Jul 5 08:46:37 2007 8, 31 -- Received: from mail131.messagelabs.com (mail131.messagelabs.com [216.82.242.99]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l65Dkaob025327 8, 31 -- for {Microscopy-at-MSA.Microscopy.com} ; Thu, 5 Jul 2007 08:46:36 -0500 8, 31 -- X-VirusChecked: Checked 8, 31 -- X-Env-Sender: Jane.LaGoy-at-Bodycote.com 8, 31 -- X-Msg-Ref: server-10.tower-131.messagelabs.com!1183643194!23275600!1 8, 31 -- X-StarScan-Version: 5.5.12.11; banners=-,-,- 8, 31 -- X-Originating-IP: [209.235.7.177] 8, 31 -- Received: (qmail 10263 invoked from network); 5 Jul 2007 13:46:34 -0000 8, 31 -- Received: from 177-209.235.7.appsitehosting.com (HELO VM200EXC02.ad.bodycote.na) (209.235.7.177) 8, 31 -- by server-10.tower-131.messagelabs.com with SMTP; 5 Jul 2007 13:46:34 -0000 8, 31 -- Received: from VM200EXC01.ad.bodycote.na ([10.200.20.9]) by VM200EXC02.ad.bodycote.na with Microsoft SMTPSVC(6.0.3790.3959); 8, 31 -- Thu, 5 Jul 2007 09:46:34 -0400 8, 31 -- Content-Class: urn:content-classes:message 8, 31 -- MIME-Version: 1.0 8, 31 -- Content-Type: text/plain; 8, 31 -- charset="us-ascii" 8, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.3959 8, 31 -- Subject: Problem with EDS signal on SEM 8, 31 -- Date: Thu, 5 Jul 2007 09:46:38 -0400 8, 31 -- Message-ID: {412949C8D462414B9BE7A0921A9C84160C22EF-at-VM200EXC01.ad.bodycote.na} 8, 31 -- X-MS-Has-Attach: 8, 31 -- X-MS-TNEF-Correlator: 8, 31 -- Thread-Topic: Problem with EDS signal on SEM 8, 31 -- thread-index: Ace/CullcuGZLSB7RiKlvXGCKzEJew== 8, 31 -- From: "Jane LaGoy" {Jane.LaGoy-at-Bodycote.com} 8, 31 -- To: {Microscopy-at-MSA.Microscopy.com} 8, 31 -- X-OriginalArrivalTime: 05 Jul 2007 13:46:34.0983 (UTC) FILETIME=[E7059770:01C7BF0A] 8, 31 -- Content-Transfer-Encoding: 8bit 8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l65Dkaob025327 ==============================End of - Headers==============================
==============================Original Headers============================== 29, 29 -- From nwwhite-at-bwxt.com Thu Jul 5 09:37:14 2007 29, 29 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 29, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l65EbDlG006029 29, 29 -- for {microscopy-at-msa.microscopy.com} ; Thu, 5 Jul 2007 09:37:13 -0500 29, 29 -- Received: from ([131.184.13.224]) 29, 29 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.5312656; 29, 29 -- Thu, 05 Jul 2007 10:36:55 -0400 29, 29 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); 29, 29 -- Thu, 5 Jul 2007 10:36:55 -0400 29, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 29, 29 -- Content-class: urn:content-classes:message 29, 29 -- MIME-Version: 1.0 29, 29 -- Content-Type: text/plain; 29, 29 -- charset="us-ascii" 29, 29 -- Subject: RE: [Microscopy] Problem with EDS signal on SEM 29, 29 -- Date: Thu, 5 Jul 2007 10:36:54 -0400 29, 29 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB7F87951-at-BWXSPO01.BWXS.BWXTECH.NET} 29, 29 -- In-Reply-To: {200707051347.l65DlguV026115-at-ns.microscopy.com} 29, 29 -- X-MS-Has-Attach: 29, 29 -- X-MS-TNEF-Correlator: 29, 29 -- Thread-Topic: [Microscopy] Problem with EDS signal on SEM 29, 29 -- Thread-Index: Ace/CyNUyAVk73R+T/WpFWh5cnEcbAAA8y0g 29, 29 -- References: {200707051347.l65DlguV026115-at-ns.microscopy.com} 29, 29 -- From: "White, Woody N." {NWWhite-at-bwxt.com} 29, 29 -- To: {Jane.LaGoy-at-Bodycote.com} , 29, 29 -- "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 29, 29 -- X-OriginalArrivalTime: 05 Jul 2007 14:36:55.0317 (UTC) FILETIME=[EF47FC50:01C7BF11] 29, 29 -- Content-Transfer-Encoding: 8bit 29, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l65EbDlG006029 ==============================End of - Headers==============================
--- randy-nessler-at-uiowa.edu wrote: } I'm posting for a colleague who is interested in } finding various vendors } of the technology listed below. Obviously, vendors } are encouraged to } reply. Anybody have vendor/system recommendations? } Randy } } } Who manufactures micro IR and Raman systems (various } combinations of } NIR, mid IR, far IR, Raman) for our existing light } microscopes? It } shouldn't necessarily be a system that is mounted } right on top of the } microscope (as in Smiths detection case). There are } bench top systems } that can be coupled to a light microscope as well. } } ----------------------------------------------------------- } Jonas Baltrusaitis, Ph.D. } Postdoctorate Research Fellow } Department of Chemistry } and } Central Microscopy Research Facility } 85 EMRB } University of Iowa } Iowa City, IA 52242 } phone: 319-335-8142 } } } ==============================Original } Headers============================== } 5, 23 -- From randy-nessler-at-uiowa.edu Tue Jul 3 } 12:44:41 2007 } 5, 23 -- Received: from } hc-smtp1.healthcare.uiowa.edu } (hc-smtp1.healthcare.uiowa.edu [129.255.210.32]) } 5, 23 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l63Hifb4012603 } 5, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 3 } Jul 2007 12:44:41 -0500 } 5, 23 -- Received: from } HC-MAIL11.healthcare.uiowa.edu ([129.255.112.33]) by } hc-smtp1.healthcare.uiowa.edu with Microsoft } SMTPSVC(6.0.3790.1830); } 5, 23 -- Tue, 3 Jul 2007 12:44:40 -0500 } 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange } V6.5 } 5, 23 -- Content-class: urn:content-classes:message } 5, 23 -- MIME-Version: 1.0 } 5, 23 -- Content-Type: text/plain; } 5, 23 -- charset="us-ascii" } 5, 23 -- Subject: Micro IR and Ramam systems } 5, 23 -- Date: Tue, 3 Jul 2007 12:44:40 -0500 } 5, 23 -- Message-ID: } {CBAD8B8185C05346A5B74AE1E12CBF2A65584C-at-HC-MAIL11.healthcare.uiowa.edu} } 5, 23 -- X-MS-Has-Attach: } 5, 23 -- X-MS-TNEF-Correlator: } 5, 23 -- Thread-Topic: Micro IR and Ramam systems } 5, 23 -- Thread-Index: } Ace9mdUL829m68CWSJGMJjTEV6xDog== } 5, 23 -- From: "Nessler, Randy A" } {randy-nessler-at-uiowa.edu} } 5, 23 -- To: {Microscopy-at-microscopy.com} } 5, 23 -- X-OriginalArrivalTime: 03 Jul 2007 } 17:44:40.0964 (UTC) FILETIME=[D54DCC40:01C7BD99] } 5, 23 -- Content-Transfer-Encoding: 8bit } 5, 23 -- X-MIME-Autoconverted: from quoted-printable } to 8bit by ns.microscopy.com id l63Hifb4012603 } ==============================End of - } Headers============================== }
____________________________________________________________________________________ Shape Yahoo! in your own image. Join our Network Research Panel today! http://surveylink.yahoo.com/gmrs/yahoo_panel_invite.asp?a=7
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} 5. Damaged detector from warm-up with bias applied.
Also unlikely.
} 6. Missing electron trap on end of detector.
What? It just fell off? Also not likely.
} 7. Ground loop in signal wires. E.G: broken cable shield, } disconnected ground wires. (Has the wiring layout changed or any } electrically noisy new equipment been installed anywhere nearby?)
Did something change in the wiring or layout? This can have a negative impact.
} ...All that comes to mind at the moment. Good Luck! } } Woody } } NW (Woody) White Jr } BWXT Services } Lynchburg, VA } http://www.bwxt.com/operations/semlab.html
Peaks at low eV can be due to noise and/or shifts in BLM. Change to a lower uS time constant and see what happens. If the same condition persists, then you have a hardware problem. Hopefully, you have a maintenance contract with EDAX. That should fix it.
gary g.
==============================Original Headers============================== 27, 20 -- From gary-at-gaugler.com Thu Jul 5 21:09:38 2007 27, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 27, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l6629cDe011421 27, 20 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jul 2007 21:09:38 -0500 27, 20 -- Message-Id: {200707060209.l6629cDe011421-at-ns.microscopy.com} 27, 20 -- Received: (qmail 6261 invoked from network); 5 Jul 2007 19:09:37 -0700 27, 20 -- Received: by simscan 1.1.0 ppid: 6258, pid: 6259, t: 0.0767s 27, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 27, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 27, 20 -- by qsmtp3 with SMTP; 5 Jul 2007 19:09:37 -0700 27, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 27, 20 -- Date: Thu, 05 Jul 2007 19:09:39 -0800 27, 20 -- To: NWWhite-at-bwxt.com 27, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 27, 20 -- Subject: Re: [Microscopy] RE: Problem with EDS signal on SEM 27, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 27, 20 -- In-Reply-To: {200707051438.l65Ecfpu008815-at-ns.microscopy.com} 27, 20 -- References: {200707051438.l65Ecfpu008815-at-ns.microscopy.com} 27, 20 -- Mime-Version: 1.0 27, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-2DE7638A ==============================End of - Headers==============================
We have a Sorvall knife breaker used to break the thicker "Ralph knives" for use with a Sorvall JB-4 microtome. It is having problems so I am looking for a manual that may give some use instructions and schematics.
Alternatively we may need to find someone to repair it. I would appreciate hearing from or about anyone who works on these instruments.
I might also be interested in buying a replacement if anyone has one sitting around not being used.
Thanks, Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907
==============================Original Headers============================== 8, 21 -- From dsherman-at-purdue.edu Thu Jul 5 21:10:26 2007 8, 21 -- Received: from 1061exfe03.adpc.purdue.edu (1061exfe03.adpc.purdue.edu [128.210.63.225]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l662AQrm012367 8, 21 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jul 2007 21:10:26 -0500 8, 21 -- Received: from exchange.purdue.edu ([172.21.6.24]) by 1061exfe03.adpc.purdue.edu with Microsoft SMTPSVC(6.0.3790.211); 8, 21 -- Thu, 5 Jul 2007 22:10:25 -0400 8, 21 -- Received: from 74.140.44.52 ([74.140.44.52]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 8, 21 -- Fri, 6 Jul 2007 02:10:24 +0000 8, 21 -- User-Agent: Microsoft-Entourage/11.3.3.061214 8, 21 -- Date: Thu, 05 Jul 2007 22:10:12 -0400 8, 21 -- Subject: Sorvall JB-4 knife breaker 8, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 8, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 8, 21 -- Message-ID: {C2B31CC4.EEDF%dsherman-at-purdue.edu} 8, 21 -- Thread-Topic: Sorvall JB-4 knife breaker 8, 21 -- Thread-Index: Ace/csjWB5vcvytmEdyGuwAKlcoUxg== 8, 21 -- Mime-version: 1.0 8, 21 -- Content-type: text/plain; 8, 21 -- charset="US-ASCII" 8, 21 -- Content-transfer-encoding: 7bit 8, 21 -- X-OriginalArrivalTime: 06 Jul 2007 02:10:26.0023 (UTC) FILETIME=[D131D770:01C7BF72] ==============================End of - Headers==============================
We have problems of switching EDS from the embedded to the stand-alone operation in our F20 STEM. We used to use EDS as a embedded system through TIA interface, and we now want to use switching capability in the TIA software, which provides a checkbox to switch from the embedded to stand-alone, external EDS collection system. The checkbox, however, did not work. To switch, we have to change parameters in engineering mode then restart the control computer, which means you have to begin from the emission start. Is there anyone who are using the EDS switching function in the TIA software? Or information about any method other than restarting the computer would be appreciated.
Young-Woon Kim Associate Professor Seoul National University School of Materials Science and Engineering Tel) +82-2-880-7977 Fax) +82-2-883-8197 e-mail) youngwk-at-snu.ac.kr
==============================Original Headers============================== 6, 28 -- From youngwk-at-snu.ac.kr Sun Jul 8 20:52:07 2007 6, 28 -- Received: from nabi2.snu.ac.kr (nabi2.snu.ac.kr [147.46.10.150]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l691q6G5026216 6, 28 -- for {Microscopy-at-microscopy.com} ; Sun, 8 Jul 2007 20:52:06 -0500 6, 28 -- Received: from [147.46.10.145] ([147.46.10.145]) 6, 28 -- by nabi2.snu.ac.kr ([147.46.10.150]) 6, 28 -- with ESMTP id 2007070910:52:04:570935.16957.2350463904 6, 28 -- for {Microscopy-at-microscopy.com} ; 6, 28 -- Mon, 09 Jul 2007 10:52:04 +0900 (KST) 6, 28 -- Received: from [147.46.233.36] ([147.46.233.36]) 6, 28 -- by auk1.snu.ac.kr ([147.46.10.145]) 6, 28 -- with ESMTP id 2007070910:51:58:158980.28252.59317152 6, 28 -- for {Microscopy-at-microscopy.com} ; 6, 28 -- Mon, 09 Jul 2007 10:51:58 +0900 (KST) 6, 28 -- From: "Young-Woon Kim" {youngwk-at-snu.ac.kr} 6, 28 -- To: {Microscopy-at-microscopy.com} 6, 28 -- Subject: EDS switching in F20 6, 28 -- Date: Mon, 9 Jul 2007 10:51:16 +0900 6, 28 -- Message-ID: {012a01c7c1cb$a34d4320$24e92e93-at-young323} 6, 28 -- MIME-Version: 1.0 6, 28 -- Content-Type: text/plain; 6, 28 -- charset="us-ascii" 6, 28 -- Content-Transfer-Encoding: 7bit 6, 28 -- X-Mailer: Microsoft Office Outlook 11 6, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 6, 28 -- Thread-Index: AcfByiKl6WZ4qCEfR2mM2yNUldFWoQ== 6, 28 -- X-TERRACE-SPAMMARK: NO (SR:12.80) 6, 28 -- (by Terrace) ==============================End of - Headers==============================
Well, I've received two offers from promising schools locally who would be willing to take on both me and my lab. They are high school programs, so I will be able to pick up right where I left off on developing my microscopy course.
I have acquired another donation of an SEM, this time a JEOL JSM-35C. I'll post pictures and the website to them when it gets here. This time, I've got an EDS system with it, so I might actually be able to do some imaging!
Now, the request. I also have a Topcon/ISI ABT-SX40A that needs a little help. I have reduced the problem down to a fault in the HV stablizer board, part number N110HC01. If anybody has this part hanging around, I would appreciate it.
The second option is for me to go ahead and digitize the scope. I know what the base voltages are going to the HV tank, and I have been able to control the emission current manually with a DC power supply injecting voltage into the circuit, but I don't have a D/A conversion board that would be able to control the outputs. Any suggestions on cheap ones?
Thanks,
Justin A. Kraft
==============================Original Headers============================== 6, 26 -- From kraftpiano-at-gmail.com Sun Jul 8 21:16:41 2007 6, 26 -- Received: from wa-out-1112.google.com (wa-out-1112.google.com [209.85.146.183]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l692Gf0P005601 6, 26 -- for {microscopy-at-microscopy.com} ; Sun, 8 Jul 2007 21:16:41 -0500 6, 26 -- Received: by wa-out-1112.google.com with SMTP id v27so1147329wah 6, 26 -- for {microscopy-at-microscopy.com} ; Sun, 08 Jul 2007 19:16:41 -0700 (PDT) 6, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 6, 26 -- d=gmail.com; s=beta; 6, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 6, 26 -- b=Dxl1uoWCupt9Q9rJ8e5gQQ/8q3ry7we2Cf1auPMDSnWKnKmOJY4fk6xsEhE7reXPwAqMu+swSLlO2CAUFgE/YHS8C2jFNqLmEHTk5eNz+vq41jXKCVeDMpbCf3RRgHxLbqIXvQoaApiduXCT8BXtX8k/dD6ABCZjx+bw1PDNtkg= 6, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 26 -- d=gmail.com; s=beta; 6, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 6, 26 -- b=qEQ8mWSZA6E0CGo7dfMr3SUsAQA/oY2xxvO1ZcUJlKsH6/gprNTYyxXXs9iAA89rEf3wRpHoWX8Wc2jjNFse7S5yICWlLWuHfg/Ea5ybYqhC25lErVAqDv7tefKN/bol2bn1kTP4lOm1EIKDEsS20LNLRuvqPm9gVyjIVRVB1mE= 6, 26 -- Received: by 10.115.107.1 with SMTP id j1mr2680685wam.1183947397503; 6, 26 -- Sun, 08 Jul 2007 19:16:37 -0700 (PDT) 6, 26 -- Received: by 10.114.78.15 with HTTP; Sun, 8 Jul 2007 19:16:37 -0700 (PDT) 6, 26 -- Message-ID: {25e2b0d20707081916i9fd65e9o1613596a411054c9-at-mail.gmail.com} 6, 26 -- Date: Sun, 8 Jul 2007 22:16:37 -0400 6, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 6, 26 -- To: microscopy-at-microscopy.com 6, 26 -- Subject: SEM Donation, Round 2, and a part request. 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 26 -- Content-Transfer-Encoding: 7bit 6, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
As time goes by, and equipment gets older, the need to repair gets greater. Does anyone have a good source for repairing the wire side bar for the old Philips specimen holders?
help would be very greatly appreciated.
Paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-3926
==============================Original Headers============================== 5, 18 -- From paul_hazelton-at-umanitoba.ca Mon Jul 9 08:50:06 2007 5, 18 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l69Do52Y030014 5, 18 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jul 2007 08:50:05 -0500 5, 18 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 5, 18 -- (authenticated bits=0) 5, 18 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id l69Dnojn017293; 5, 18 -- Mon, 9 Jul 2007 08:50:02 -0500 (CDT) 5, 18 -- Message-ID: {46923C86.5070901-at-umanitoba.ca} 5, 18 -- Date: Mon, 09 Jul 2007 08:47:50 -0500 5, 18 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 5, 18 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 5, 18 -- X-Accept-Language: en-us, en 5, 18 -- MIME-Version: 1.0 5, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 5, 18 -- Subject: specimen holder repair 5, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We've been sending our holders to Tom Schmeltzer for repair and been quite happy with his work.
Tom Schmeltzer TGS Technologies 900 Glenwood Ct. Cranberry Township, PA 16066 (724) 453-3865
...just a satisfied customer...
Cheers, Henk
At 09:52 AM 07/09/07, paul_hazelton-at-umanitoba.ca wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
-----Original Message----- X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu] Sent: Monday, July 09, 2007 10:56 AM To: Williams, Geoffrey
Hi Paul,
We've been sending our holders to Tom Schmeltzer for repair and been quite happy with his work.
Tom Schmeltzer TGS Technologies 900 Glenwood Ct. Cranberry Township, PA 16066 (724) 453-3865
...just a satisfied customer...
Cheers, Henk
At 09:52 AM 07/09/07, paul_hazelton-at-umanitoba.ca wrote:
} ----------------------------------------------------------------------- ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
I am looking for commercial labs equipped with good FEG-STEM, which is able to do EDS analysis. The STEM probe size is preferred to be ~0.5-1 nm, and it would be good to have high angle annular dark filed image available.
If you have any information about it, please let me know. Your help will be highly appreciated.
Juan
==============================Original Headers============================== 7, 26 -- From jcai-at-nanostellar.com Mon Jul 9 12:23:54 2007 7, 26 -- Received: from mail.nanostellar.com (mail.nanostellar.com [207.212.42.99]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l69HNsMe006237 7, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jul 2007 12:23:54 -0500 7, 26 -- Received: from localhost (localhost [127.0.0.1]) 7, 26 -- by mail.nanostellar.com (Postfix) with ESMTP id 29CAC2D306; 7, 26 -- Mon, 9 Jul 2007 10:23:56 -0700 (PDT) 7, 26 -- Received: from mail.nanostellar.com ([127.0.0.1]) 7, 26 -- by localhost (gemini.nanostellar.com [127.0.0.1]) (amavisd-new, port 10024) 7, 26 -- with ESMTP id 21503-10; Mon, 9 Jul 2007 10:23:55 -0700 (PDT) 7, 26 -- Received: from gemini.nanostellar.com (localhost [127.0.0.1]) 7, 26 -- by mail.nanostellar.com (Postfix) with ESMTP id 9C80113D0 7, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jul 2007 10:23:55 -0700 (PDT) 7, 26 -- Message-ID: {31517012.451184001835636.OPEN-XCHANGE.WebMail.tomcat-at-gemini.nanostellar.com} 7, 26 -- Date: Mon, 9 Jul 2007 10:23:55 -0700 (PDT) 7, 26 -- From: Juan Cai {jcai-at-nanostellar.com} 7, 26 -- To: microscopy-at-microscopy.com 7, 26 -- Subject: commercial lab with state of the art FEG-STEM 7, 26 -- MIME-Version: 1.0 7, 26 -- Content-Type: text/plain; charset=UTF-8 7, 26 -- X-Priority: 3 (normal) 7, 26 -- X-Mailer: OPEN-XCHANGE 5607 - WebMail 7, 26 -- Organization: Nanostellar Inc. 7, 26 -- X-Virus-Scanned: by amavisd-new at nanostellar.com 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l69HNsMe006237 ==============================End of - Headers==============================
Here at long last is my promised summary of replies to my question about how to image carbon nanoparticles that have been taken up by organisms into tissue. Hope I didn't miss anybody.
Many thanks again to all who replied.
X-from Mhairi Gass: "A colleague has brought my attention to your question on visualising carbon nanos in tissue. I have been working on a similar project with Alex Porter (Cambridge University) where we have been exploiting a range of techniques to visualise C60 and SWNTs in cellular materials. We have found that staining the specimens can often add confusion, but is useful to identify the different parts of a cell. As for differentiating between the carbonaceous materials we have found that a ratio of plasmon energies in EFTEM works very well but this must be on unstained sections (i.e. EFTEM images at 26eV and 22eV ish, or even using the pi to pi* transition at 6eV for crystalline materials). I have attached a paper that was published earlier this year which may be of interest. Also HAADF imaging can enhance the contrast from the crystalline material as it has a higher density. We currently have a paper in review on unstained imaging at high resolution which involves preparing much thinner samples than are traditionally used for biological samples to minimise the 3D information, they are not easy to work with but the resolution is much improved." (The paper attached was: Visualizing the Uptake of C60 to the Cytoplasm and Nucleus of Human Monocyte-Derived Macrophage Cells Using Energy-Filtered Transmission Electron Microscopy and Electron Tomography. Alexandrae Porter, Mhairi Gass, Karin Muller, Jeremyn Skepper, Paul Midgley, and Mark Welland. Environ. Sci. Technol. 2007, 41, 3012-3017. RT) *************************
X-from Hendrik Colijn: "I don't think the nano-thingys will take much stain. You may have better luck staining the tissue and working with a sort of "negative stain" If the nanothingys are crystalline enough, you may be able to do dark field imaging." **************************
X-from Ephram Mark Shizgal: "Randy, I would love to give those sections a look under our low voltage TEM (see http://www.lv-em.com/ RT--no connection with me, etc., but interesting information) to see if the extra contrast low kV provides enhances the nanomaterials. How nano are they? I know that you guys don't have the LVEM5, but if it was something you'd want to try we would be happy to look at them at our applications lab. Let me know." ********************************
X-from Phil Oshel: "The only way I see is to incorporate C-14 into the nanobits when they're made, then use old-fashioned radio-immuno techniques. Autoradiography. Don't see how this would help with SEM, but would work for TEM and LM." ********************************
X-from Barbara Foster: "This is another one of those "TOMO" applications.
As mentioned earlier, I have a movie made by Dr.deWith of the Dutch Polymer Institute (TU/E) showing the distribution of carbon nanotubes in epoxy. Because the AFM uses local differences in elasticity, there is none of the CNT in C-based tissue problem described below. Again, although I am no longer involved in this project, I am happy to forward a copy of the film to anyone who is interested. (It will come via www.YouSendIt.com, because of the large file size).
Dr. Anton Efimov of NT-MDT is the developer of this technique and is usually willing to run samples, schedule permitting. See his email in the CC: above.
NTA is no longer NT-MDT's agent here in the US. If you are interested in following this further, I recommend that you contact Dr. Yuri Bobrov, who is now with ABeam (Dealer principle, Dr. Sergey Babin - see email above).
As always, if there are further questions, please don't hesitate to call/email.
(Note: MME is no longer involved with this vendor)." ***********************************
X-from Petra Rudolf: "Bruce Weisman (weisman-at-rice.edu) has very nicely shown that it is possible to identify the type of CNT inside tissue from fluorescence spectroscopy - one can actually see fluorescence from a single tube and from the frequency identify what tube it is.He has also shown how to do statistics of the distribution of nanotubes in different types of tissue. I heard his talk on this subject at the 2006 IWEPNM in Kirchberg, Austria." *************************************
X-from Dale Callaham: "I have looked at carbon nanotubes a bit. I have looked at them applied to thin carbon films - no neg stain necessary - and they stand out well - very easy to image - especially the multiwall ones. I have also sectioned them in epoxy and melamine resins and it was no problem to see them easily in normal 60nm sections. In the presence of complex tissue it could be more difficult to see them. But the cells may react in some way to partition them and make them easy to see?
And I have seen many different shapes/lengths. The particular sample from a source is fairly consistent - I have never purchased so I don't know what people see about what they get, but they are often surprised when I show them the images. I went into it thinking that I was going to see little soda straws, but I have seen nearly any conformation you can imagine. Some disperse easily but I had some that took much sonication to disperse, and just a little swirl and they came out of suspension; those were the long curly ones - they tangle easily when swirled. So get a sample from your associates and see what you are dealing with." ********************************
X-from Stephane Nizet: "Been not involved in carbon nanotube research I dont know exactly how dense they are under the beam of a TEM. However I don't think you can compare the density of the carbon present in a tissue with the density of carbon in nanotubes. The difference MUST be visible. I wouldn't modify the nanotubes themselves because you will definitely modify their behaviour in an uncontrolled manner. Now here is what I would do: I would incubate cell monolayers with the nanoparticles and flat embed them (control=cells incubated at 4°C, no uptake) after ferrocyanide-osmium post-fixation. I am pretty sure the cells will internalize the particles (take hepatocytes like HepG2 they are very effecient at uptaking and very nice to show). After sectionning, I would try different contrasting intensities and also without any contrasting at all! This way you can define your technical parameters to obtain the best contrast possible in "control" samples.
Finding these particles in organs is another story, requiring mainly eternities of patience and dedication. But, if I may give my personal opinion, this is a very interesting study and probably a very rewarding one. Few have had the heart to start it." ****************************************
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 22, 23 -- From TindallR-at-missouri.edu Mon Jul 9 15:23:28 2007 22, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 22, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l69KNSeq022190 22, 23 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jul 2007 15:23:28 -0500 22, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 22, 23 -- Mon, 9 Jul 2007 15:23:27 -0500 22, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 22, 23 -- Content-class: urn:content-classes:message 22, 23 -- MIME-Version: 1.0 22, 23 -- Content-Type: text/plain; 22, 23 -- charset="iso-8859-1" 22, 23 -- Subject: Summary: SEM/TEM--Carbon nanoparticles in tissue 22, 23 -- Date: Mon, 9 Jul 2007 15:23:28 -0500 22, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BB9D-at-UM-XMAIL08.um.umsystem.edu} 22, 23 -- X-MS-Has-Attach: 22, 23 -- X-MS-TNEF-Correlator: 22, 23 -- Thread-Topic: Summary: SEM/TEM--Carbon nanoparticles in tissue 22, 23 -- Thread-Index: AcfCZwIsV0T8CFPPR7+6bM3yPhDsfg== 22, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 22, 23 -- To: {microscopy-at-microscopy.com} 22, 23 -- X-OriginalArrivalTime: 09 Jul 2007 20:23:27.0888 (UTC) FILETIME=[02458900:01C7C267] 22, 23 -- Content-Transfer-Encoding: 8bit 22, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l69KNSeq022190 ==============================End of - Headers==============================
Thanks to all who responded to my problem with repair of specimen holders. A number of very good sources for repair and replacement were sent.
Once again, thanks
Paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-3926
==============================Original Headers============================== 6, 18 -- From paul_hazelton-at-umanitoba.ca Wed Jul 11 08:23:56 2007 6, 18 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6BDNtDV000981 6, 18 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jul 2007 08:23:56 -0500 6, 18 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 6, 18 -- (authenticated bits=0) 6, 18 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id l6BDNodm014545; 6, 18 -- Wed, 11 Jul 2007 08:23:55 -0500 (CDT) 6, 18 -- Message-ID: {4694D96F.1090805-at-umanitoba.ca} 6, 18 -- Date: Wed, 11 Jul 2007 08:21:51 -0500 6, 18 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 6, 18 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 6, 18 -- X-Accept-Language: en-us, en 6, 18 -- MIME-Version: 1.0 6, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 6, 18 -- Subject: Thanks - Specimen Holder Repair 6, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Electron/Ion Beam Instrument Service Engineer The University of Oregon's Center for Advanced Materials Characterization in Oregon (CAMCOR) is seeking applications for a full time staff position to begin September 2007. A strong background in maintaining, trouble shooting and upgrading electron/ion beam instruments and associated high voltage, vacuum, mechanical and electrical systems is required. Experience with x-ray diffraction instrumentation is also desirable. Salary range $60K-90K commensurate with experience.
This position will be located in the new Lorry Lokey Integrated Science Laboratory, a state of the art nano and micro science analytical instrument facility designed specifically for exceptional nano-science performance. It will house the latest electron, ion and x-ray beam instrumentation available including a Zeiss Ultra TFEM, FEI Quanta 200 E-SEM, Cameca SX50 and SX100 microprobes, Ion-TOF SIMS, Philips Auger Spectrometer, Philips TEM, Kratos XPS and various assorted coaters, etchers, and other vacuum deposition systems.
The successful candidate with have a BS in a beam microscopy related field and an extensive background in instrument field service with significant practical experience troubleshooting high vacuum electron and ion beam instrumentation at both the system and PC board levels. Must be able to read and understand schematics for electronic circuits and systems. The successful applicant will be involved in modifying/improving instrumentation capabilities to enable the equipment to more fully support unique research needs and will be expected to work intimately with the scientific staff and research faculty. We seek candidates with a demonstrated commitment to working effectively with students, faculty and staff from diverse backgrounds.
Interested persons should send a resume with a detailed description of work experience and skills, and arrange for two letters of recommendation to be sent to: CAMCOR Instrument Engineer Search Committee, 1253 University of Oregon, Eugene, OR, 97403-1253. To be assured of full consideration, application materials must be received by July 31, 2007, but the search will remain open until the position is filled. For further information, contact John Donovan (donovan-at-uoregon.edu).
University of Oregon is an AA/EEO employer committed to cultural diversity.
==============================Original Headers============================== 7, 20 -- From donovan-at-uoregon.edu Wed Jul 11 14:29:28 2007 7, 20 -- Received: from smtp.uoregon.edu (mserv5.uoregon.edu [128.223.142.42]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6BJTRU8021533 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jul 2007 14:29:27 -0500 7, 20 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.92.75]) 7, 20 -- (authenticated bits=0) 7, 20 -- by smtp.uoregon.edu (8.13.8/8.13.8) with ESMTP id l6BJTRdO023248 7, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jul 2007 12:29:27 -0700 7, 20 -- Message-Id: {200707111929.l6BJTRdO023248-at-smtp.uoregon.edu} 7, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 20 -- Date: Wed, 11 Jul 2007 12:29:25 -0700 7, 20 -- To: microscopy-at-microscopy.com 7, 20 -- From: John Donovan {donovan-at-uoregon.edu} 7, 20 -- Subject: Instrument Service Engineer Position at the University of 7, 20 -- Oregon 7, 20 -- Mime-Version: 1.0 7, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 20 -- X-Virus-Scanned: ClamAV 0.90.3/3637/Wed Jul 11 09:27:26 2007 on mserv5 7, 20 -- X-Virus-Status: Clean ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gas19-at-daimlerchrysler.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both WLehman-at-bu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: WLehman-at-bu.edu Name: Tori Hatch
Organization: Boston University
Title-Subject: [Filtered] EM position available
Question: POSITION AVAILABLE Senior Electron Microscopy Technician
A Senior Electron Microscopy Technician is sought to perform duties in support of research work on the structure of actin filament complexes that (1) are associated with cardiac and skeletal muscle regulatory proteins to control muscle activity and (2) interact with smooth muscle actin-binding proteins to modulate the assembly of the smooth muscle cytoskeleton. Applicants must have experience in high-resolution EM work and first-rate facility with computer-assisted image analysis. The candidate should have a Bachelorís degree and several years of experience. Prior familiarity with preserving and recording macromolecular assemblies in negative stain and by cryo-EM methods would be invaluable. Experience in supervising graduate students and post-doctoral fellows would also be important.
Interested applicants should send their CV with three references to:
Dr. William Lehman Professor of Physiology & Biophysics Department of Physiology & Biophysics Boston University School of Medicine 715 Albany Street Boston, MA 02118
Or email:
wlehman-at-bu.edu
Dr. Lehmanís research program is summarized on his home page:
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both medvitz-at-bostwicklaboratories.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: medvitz-at-bostwicklaboratories.com Name: Neil Medvitz
Organization: Bostwick Laboratories
Title-Subject: [Filtered] Uranyl acetate disposal
Question: Does anyone know of a company that will take my uranyl acetate & uranyl nitrate waste in the Richmond Virginia area?? As usual as soon as someone hears uranyl they freak out.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fittonro-at-luther.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: fittonro-at-luther.edu Name: Robert Fitton
Organization: Luther College
Title-Subject: [Filtered] Gatan 791 manual wanted
Question: Greetings, I'm looking for someone who would be willing to make a copy of the Gatan 791 manual that includes installation instructions and send it to me either snail mail or pdf. I'm specifically interested in the pneumatic connections and psi specifications. Thanks! Robert Fitton/Luther College/Decorah, IA
I have a researcher who wants to do immuno labeling, but the sample is already in gluteraldehyde.
I've always gone on the premise that gluteraldehyde is permanent, and always suggest 4% parafromaldehyde or parafromaldehyde with the barest littlest amount of gluteraldehyde ( {1%).
I am not familiar with any retrieval protocols, do you know of any for gluteraldehyde, where the Ag site actually is affected by the gluteraldehyde?
Thanks,
Lou Ann
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Lou Ann Miller, MT(ASCP) Service Supervisor Center for Microscopic Imaging College of Veterinary Medicine Rm 1204 VMBSB 2001 S Lincoln Ave Urbana, IL 61802
217/244-1567 http://treefrog.cvm.uiuc.edu
==============================Original Headers============================== 14, 17 -- From lamiller-at-uiuc.edu Thu Jul 12 12:43:08 2007 14, 17 -- Received: from expredir4.cites.uiuc.edu (expredir4.cites.uiuc.edu [128.174.5.187]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6CHh8Kh028959 14, 17 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jul 2007 12:43:08 -0500 14, 17 -- Received: from redkite.cvm.uiuc.edu (redkite.cvm.uiuc.edu [130.126.18.157]) 14, 17 -- by expredir4.cites.uiuc.edu (8.14.1/8.14.1) with ESMTP id l6CHh8Mf003938 14, 17 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jul 2007 12:43:08 -0500 (CDT) 14, 17 -- Message-ID: {4696682D.20101-at-uiuc.edu} 14, 17 -- Date: Thu, 12 Jul 2007 12:43:09 -0500 14, 17 -- From: Lou Ann Miller {lamiller-at-uiuc.edu} 14, 17 -- Reply-To: lamiller-at-uiuc.edu 14, 17 -- User-Agent: Thunderbird 2.0.0.4 (Macintosh/20070604) 14, 17 -- MIME-Version: 1.0 14, 17 -- To: microscopy-at-microscopy.com 14, 17 -- Subject: Taking the gluteraldehyde out 14, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Yes, this is what I told the person, I remember learning from Bendyan that insulin is this way. ( and don't we wish that all antigens were that strong!!)
I will talk to the researcher more, I suspect they may be asking because their immuno didn't work as he was concerned about retrieval, but I'll find out if he tried it anyway.
Lou Ann
Webster, Paul wrote: } Dear Lou Ann, } } The rule that glutaraldehyde should not be used for immunolabeling is not } absolute. Before attempting all sorts of different retrieval techniques why } not give the immunolabeling a try. If the antigen is fairly abundant and not } surrounded by lots of other proteins, you may find it works. } } Once you have a result from the simple immunolabeling experiment, and it } shows not signal, then try complicating things by attempting antigen } retrieval. } } There are lots of examples in the literature where high concentrations of } glutaraldhyde were used for immunolabeling (and even Epo-embedded tissues). } } Paul. } } } } } } From: {lamiller-at-uiuc.edu} } } Reply-To: {lamiller-at-uiuc.edu} } } Date: Thu, 12 Jul 2007 12:54:43 -0500 } } To: {pwebster-at-hei.org} } } Subject: [Microscopy] Taking the gluteraldehyde out } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear Folks on the Microscopy List, } } } } } } I have a researcher who wants to do immuno labeling, but the sample is } } already in gluteraldehyde. } } } } I've always gone on the premise that gluteraldehyde is permanent, and } } always suggest 4% parafromaldehyde or parafromaldehyde with the barest } } littlest amount of gluteraldehyde ( {1%). } } } } } } I am not familiar with any retrieval protocols, do you know of any for } } gluteraldehyde, where the Ag site actually is affected by the } } gluteraldehyde? } } } } } } } } Thanks, } } } } } } Lou Ann } } } } -- } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } Lou Ann Miller, MT(ASCP) } } Service Supervisor } } Center for Microscopic Imaging } } College of Veterinary Medicine } } Rm 1204 VMBSB } } 2001 S Lincoln Ave } } Urbana, IL 61802 } } } } 217/244-1567 } } http://treefrog.cvm.uiuc.edu } } } } } } ==============================Original Headers============================== } } 14, 17 -- From lamiller-at-uiuc.edu Thu Jul 12 12:43:08 2007 } } 14, 17 -- Received: from expredir4.cites.uiuc.edu (expredir4.cites.uiuc.edu } } [128.174.5.187]) } } 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } l6CHh8Kh028959 } } 14, 17 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jul 2007 12:43:08 -0500 } } 14, 17 -- Received: from redkite.cvm.uiuc.edu (redkite.cvm.uiuc.edu } } [130.126.18.157]) } } 14, 17 -- by expredir4.cites.uiuc.edu (8.14.1/8.14.1) with ESMTP id } } l6CHh8Mf003938 } } 14, 17 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jul 2007 12:43:08 -0500 } } (CDT) } } 14, 17 -- Message-ID: {4696682D.20101-at-uiuc.edu} } } 14, 17 -- Date: Thu, 12 Jul 2007 12:43:09 -0500 } } 14, 17 -- From: Lou Ann Miller {lamiller-at-uiuc.edu} } } 14, 17 -- Reply-To: lamiller-at-uiuc.edu } } 14, 17 -- User-Agent: Thunderbird 2.0.0.4 (Macintosh/20070604) } } 14, 17 -- MIME-Version: 1.0 } } 14, 17 -- To: microscopy-at-microscopy.com } } 14, 17 -- Subject: Taking the gluteraldehyde out } } 14, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 14, 17 -- Content-Transfer-Encoding: 7bit } } ==============================End of - Headers============================== } } } } }
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Lou Ann Miller, MT(ASCP) Service Supervisor Center for Microscopic Imaging College of Veterinary Medicine Rm 1204 VMBSB 2001 S Lincoln Ave Urbana, IL 61802
217/244-1567 http://treefrog.cvm.uiuc.edu
==============================Original Headers============================== 9, 19 -- From lamiller-at-uiuc.edu Thu Jul 12 13:04:40 2007 9, 19 -- Received: from expredir4.cites.uiuc.edu (expredir4.cites.uiuc.edu [128.174.5.187]) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6CI4djI008640 9, 19 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jul 2007 13:04:40 -0500 9, 19 -- Received: from redkite.cvm.uiuc.edu (redkite.cvm.uiuc.edu [130.126.18.157]) 9, 19 -- by expredir4.cites.uiuc.edu (8.14.1/8.14.1) with ESMTP id l6CI4dFs011549; 9, 19 -- Thu, 12 Jul 2007 13:04:39 -0500 (CDT) 9, 19 -- Message-ID: {46966D38.1090401-at-uiuc.edu} 9, 19 -- Date: Thu, 12 Jul 2007 13:04:40 -0500 9, 19 -- From: Lou Ann Miller {lamiller-at-uiuc.edu} 9, 19 -- Reply-To: lamiller-at-uiuc.edu 9, 19 -- User-Agent: Thunderbird 2.0.0.4 (Macintosh/20070604) 9, 19 -- MIME-Version: 1.0 9, 19 -- To: "Webster, Paul" {PWebster-at-hei.org} , microscopy-at-microscopy.com 9, 19 -- Subject: Re: [Microscopy] Taking the gluteraldehyde out 9, 19 -- References: {C2BBB9F8.1296A%PWebster-at-hei.org} 9, 19 -- In-Reply-To: {C2BBB9F8.1296A%PWebster-at-hei.org} 9, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
New York Microscopical Society Bernard Friedman Memorial Workshop Digital Photomicrography with the Light Microscope August 21, 22 & 23, 2007
A hands-on workshop using professional microscopes and cameras designed for photomicrography combined with the theory of their use.
This workshop teaches the fundamentals of taking digital micrographs through transmitted light microscopes for illustrative purposes. Typical subjects covered will be:
Getting the Microscope Ready for Image Recording Centering of Objectives and Condenser Koehler Illumination Significance of the Aperture Magnification and Resolution Parfocality of Camera and Visualized Image
Digital Camera and Driver Electronic image sensors Histograms Gamma Settings White Balance Background Subtraction Compression of Image Files
Imaging Software used in the Course Photoshop Elements: ImageJ: Measurements of length and area, scale bar
Integration of Digital Images into Documents Microsoft Word: Importing images into MS Word
Instructors Jan Hinsch, formerly of Leica Microsystems, Dr. Angela Klaus, Seton Hall University Mel Pollinger, Boston Scientific, Don O'Leary, NYMS .
The Workshop will be held at NYMS Headquarters at 30 N. Mountain Avenue, Montclair, NJ from 9:00 AM to 5:00 PM. Lunch will be supplied Cost will be $ 550 For Members $ 580 for nonmembers (includes membership) Attendance is limited to the first 12 applicants.
For further information e-mail dkoleary-at-verizon.net or call (201) 368-8849
To register please return the form below with payment to: NYMS, C/o Donald O'Leary, 10 Sampson Street. Unit 113, Saddle Brook, NJ 07663 _____________________________________________________ Digital Photomicrography with the Light Microscope August 21, 22 & 23, 2007
Name_________________________________________________________________ Address_______________________________________________________________ City____________________________State_____________Zip_____________ Home Phone__________________________Wrk Phone____________________ e-Mail__________________________________________________________
Don O'Leary Treasurer EAS 2007
==============================Original Headers============================== 12, 20 -- From dkoleary-at-verizon.net Thu Jul 12 13:50:06 2007 12, 20 -- Received: from smtp102.vzn.mail.dcn.yahoo.com (smtp102.vzn.mail.dcn.yahoo.com [209.73.179.140]) 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l6CIo6LQ022110 12, 20 -- for {Microscopy-at-Microscopy.Com} ; Thu, 12 Jul 2007 13:50:06 -0500 12, 20 -- Received: (qmail 67815 invoked from network); 12 Jul 2007 18:50:06 -0000 12, 20 -- Received: from unknown (HELO DonsLaptop) (dkoleary-at-verizon.net-at-68.239.182.77 with login) 12, 20 -- by smtp102.vzn.mail.dcn.yahoo.com with SMTP; 12 Jul 2007 18:50:05 -0000 12, 20 -- X-YMail-OSG: HG3DluUVM1mk1xErAMz6FHBRRPgcVFzKKdD0KxR.XWQW2ojendusCuGJr4AXDARNSUjM_NuZRQ-- 12, 20 -- From: "Donald O'Leary" {dkoleary-at-verizon.net} 12, 20 -- To: "Microscopy List Server" {Microscopy-at-Microscopy.Com} 12, 20 -- Subject: LM Workshop on Digital Photomicrography 12, 20 -- Date: Thu, 12 Jul 2007 14:50:05 -0400 12, 20 -- Message-ID: {000f01c7c4b5$766c4440$0200a8c0-at-DonsLaptop} 12, 20 -- MIME-Version: 1.0 12, 20 -- Content-Type: text/plain; 12, 20 -- charset="us-ascii" 12, 20 -- Content-Transfer-Encoding: 7bit 12, 20 -- X-Mailer: Microsoft Office Outlook 11 12, 20 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 12, 20 -- Thread-index: AcfEtXW+anymAgF5TPi0KKUgcjIVAQ== ==============================End of - Headers==============================
We've been having problems for years getting flat illumination across our images with a Zeiss AxioSkopII with a Zeiss Axiocam.
The unevenness is not noticeable when we have high contrast histological stains, but with very low contrast samples, the corners of the images are noticeably dark.
We've identified one source of the unevenness that causes problems specifically at the right edges of our images. The ND filters in the illumination light path reflect off each other (examples at http://www.aecom.yu.edu/aif/temp/ZeissAxioSkopII/ ). If we take out the ND filters, the field is far more even; however, the light is too bright and turning down the voltage to the halogen lamp throws off the color temperature.
Also, the unevenness is not gone ocmpletely.
Has anybody else noticed this problem and, if so, how have you solved it?
Thanks.
-Michael ____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. URL: http://www.aecom.yu.edu/aif/
==============================Original Headers============================== 8, 28 -- From cammer-at-aecom.yu.edu Thu Jul 12 16:22:27 2007 8, 28 -- Received: from mx1.aecom.yu.edu (mx1.aecom.yu.edu [129.98.1.51]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6CLMRKF005022 8, 28 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jul 2007 16:22:27 -0500 8, 28 -- Received: from draco.aecom.yu.edu (draco.aecom.yu.edu [129.98.1.160]) 8, 28 -- by mx1.aecom.yu.edu (Postfix) with ESMTP id 01B6C9F00C2 8, 28 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jul 2007 17:22:27 -0400 (EDT) 8, 28 -- Received: from draco.aecom.yu.edu (unknown [127.0.0.1]) 8, 28 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 2F5B58B4002 8, 28 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jul 2007 17:13:20 -0400 (EDT) 8, 28 -- X-AuditID: 816201a0-a2ccabb000005b7e-78-4696996fdbc8 8, 28 -- Received: from post.aecom.yu.edu (post.aecom.yu.edu [129.98.1.100]) 8, 28 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id EEBCE718002 8, 28 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jul 2007 17:13:19 -0400 (EDT) 8, 28 -- Received: from AIF3.aecom.yu.edu (aif3.aif.aecom.yu.edu [129.98.80.70]) 8, 28 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 8, 28 -- (No client certificate requested) 8, 28 -- by post.aecom.yu.edu (Postfix) with ESMTP id 8493331 8, 28 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jul 2007 17:22:26 -0400 (EDT) 8, 28 -- Message-Id: {7.0.1.0.2.20070712171754.022e6e68-at-aecom.yu.edu} 8, 28 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 8, 28 -- Date: Thu, 12 Jul 2007 17:22:15 -0400 8, 28 -- To: microscopy-at-microscopy.com 8, 28 -- From: Michael Cammer {cammer-at-aecom.yu.edu} 8, 28 -- Subject: Zeiss Axioskop II uneven illumination 8, 28 -- Mime-Version: 1.0 8, 28 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 28 -- X-Brightmail-Tracker: AAAAAA== ==============================End of - Headers==============================
A few weeks ago I posed a question about how to take a look at some bones for a quasi-CSI type project. I received several kind and thoughtful replies, including some that suggested using an environmental SEM.
Well, duh, what a good idea. Problem is, I don't have an ESEM and am at a loss as to how to get one. Thus today's question.
How are new instruments funded? I have always assumed that some big time PI would get grants and new instruments would just come rolling in. Maybe this works if you have high rollers, and maybe it works for their own labs, but what about a low rolling central campus facility that does a little of this and a little of that for everyone?
We really don't have the critical mass needed to leverage much of an extramural grant and our instruments are all approaching 25 years old. They still work, but are not modern or up to date by any means.
If I ask for $$ from the Dean, the answer is usually 'Wow! I didn't know they cost so much. Can you get by with what you have? There is an emergency in blah blah's lab and we are out of money for this year.' Kind of a management by crisis strategy. Is it like this everywhere? Does anyone really have any long term infrastructural planning in place?
So, does anyone know if there are actual institutions that plan for replacing old equipment with institutional funds or are all microscopes funded by extramural grants? I know about cost sharing, but without the significant projects and faculty interest, we never get that far. Sometimes I think I know how that slide rule salesman must have felt when HP calculators hit the market.
Today I am faced with the prospect of spending $10K or so if I can get the funding from the Dean to repair a 25 year old SEM or replace it with a 'new', only 15 year old, used SEM I picked up as a donation for about the same cost. Neither one would be an ESEM, and neither one could be considered up to date.
What's a body to do? Enquiring minds want to know!
Thanks
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. Visit http://www.aidslifecycle.org for more information about the ride or http://www.aidslifecycle.org/5482 to make a donation.
==============================Original Headers============================== 13, 17 -- From jmkrupp-at-ucsc.edu Thu Jul 12 16:57:31 2007 13, 17 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 13, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6CLvVi3017404 13, 17 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jul 2007 16:57:31 -0500 13, 17 -- Received: from [128.114.125.5] (HELO ucsc.edu) 13, 17 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 13, 17 -- with ESMTPS id 20409427 for microscopy-at-microscopy.com; Thu, 12 Jul 2007 14:57:30 -0700 13, 17 -- Received: from [128.114.25.127] (account jmkrupp-at-ucsc.edu HELO [128.114.25.127]) 13, 17 -- by copper.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 13, 17 -- with ESMTPA id 139311382 for microscopy-at-microscopy.com; Thu, 12 Jul 2007 14:57:30 -0700 13, 17 -- Mime-Version: 1.0 13, 17 -- Message-Id: {p06230904c2bc4868f1f0-at-[128.114.25.127]} 13, 17 -- Date: Thu, 12 Jul 2007 14:57:28 -0700 13, 17 -- To: microscopy-at-microscopy.com 13, 17 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 13, 17 -- Subject: Funding equipment 13, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both anita.garg-at-grc.nasa.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Veleta: TEM CCD camera??
Question: We are planning to buy a side mounted CCD camera for our CM200 TEM. We are considering the "2k X 2k Veleta" from Olympus SIS. We would like to hear the comments from the experienced users. Any suggestions regarding the "Diffraction Software" would be highly appreciated too. TIA Anita
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cvierret-at-umr.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: cvierret-at-umr.edu Name: Clarissa Wisner
Organization: University of Missouri At Rolla, for now
Title-Subject: [Filtered] Measuring Beam Diameter
Question: Hello,
I have been charged with measuring the beam diameter in our scopes. I have researched some methods and most involve a Si knife. Do any of you have a method that you have used to measure beam diameter and if so where did you obtain your materials. Any and all information would be appreciated.
Thanks
Clarissa Wisner SEM Specialist G6 MRC University of Missouri Rolla, Mo 65409 cvierret-at-umr.edu 573-341-4393
First of all, as a neutral using all sorts of SEM from all the manufacturers, if you have very little money or operating time to spend with the instrument do not get hung up on an ESEM.
There are a mass of simple techniques that allow us to look at even the most difficult specimens on an "ordinary" SEM. A conventional instrument is far more likely to be available than a dedicated ESEM and most well known models, even 15 to 20 years old, will serve you very well.
I have just run a course on a 20 year old Camscan in its new (second hand) home, complete with EDS, it is more than capable of imaging at 30,000X.
Good luck
Steve Chapman Senior Consultant Protrain For electron microscopy consultancy and training world wide Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967 Web www.emcourses.com
----- Original Message ----- X-from: {jmkrupp-at-ucsc.edu} To: {protrain-at-emcourses.com} Sent: Thursday, July 12, 2007 11:00 PM
Hi Lou Ann,
The chances are weak but it is definitely worth a try. However one important problem with glutar fixation is that it gives autofluorescence, so use another detection method. It is claimed that NaBH4 can block the autofluorescence but it didn't really work with me (probably depends on the intensity and pattern of the specific labeling).
Stephane
--- lamiller-at-uiuc.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Thanks Paul, } } Yes, this is what I told the person, I remember } learning from Bendyan } that insulin is this way. ( and don't we wish that } all antigens were } that strong!!) } } } I will talk to the researcher more, I suspect they } may be asking because } their immuno didn't work as he was concerned about } retrieval, but I'll } find out if he tried it anyway. } } Lou Ann } } Webster, Paul wrote: } } Dear Lou Ann, } } } } The rule that glutaraldehyde should not be used } for immunolabeling is not } } absolute. Before attempting all sorts of different } retrieval techniques why } } not give the immunolabeling a try. If the antigen } is fairly abundant and not } } surrounded by lots of other proteins, you may find } it works. } } } } Once you have a result from the simple } immunolabeling experiment, and it } } shows not signal, then try complicating things by } attempting antigen } } retrieval. } } } } There are lots of examples in the literature where } high concentrations of } } glutaraldhyde were used for immunolabeling (and } even Epo-embedded tissues). } } } } Paul. } } } } } } } } } } } From: {lamiller-at-uiuc.edu} } } } Reply-To: {lamiller-at-uiuc.edu} } } } Date: Thu, 12 Jul 2007 12:54:43 -0500 } } } To: {pwebster-at-hei.org} } } } Subject: [Microscopy] Taking the gluteraldehyde } out } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } ---------------------------------------------------------------------------- } } } } } } Dear Folks on the Microscopy List, } } } } } } } } } I have a researcher who wants to do immuno } labeling, but the sample is } } } already in gluteraldehyde. } } } } } } I've always gone on the premise that } gluteraldehyde is permanent, and } } } always suggest 4% parafromaldehyde or } parafromaldehyde with the barest } } } littlest amount of gluteraldehyde ( {1%). } } } } } } } } } I am not familiar with any retrieval protocols, } do you know of any for } } } gluteraldehyde, where the Ag site actually is } affected by the } } } gluteraldehyde? } } } } } } } } } } } } Thanks, } } } } } } } } } Lou Ann } } } } } } -- } } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } } Lou Ann Miller, MT(ASCP) } } } Service Supervisor } } } Center for Microscopic Imaging } } } College of Veterinary Medicine } } } Rm 1204 VMBSB } } } 2001 S Lincoln Ave } } } Urbana, IL 61802 } } } } } } 217/244-1567 } } } http://treefrog.cvm.uiuc.edu } } } } } } } } } ==============================Original } Headers============================== } } } 14, 17 -- From lamiller-at-uiuc.edu Thu Jul 12 } 12:43:08 2007 } } } 14, 17 -- Received: from expredir4.cites.uiuc.edu } (expredir4.cites.uiuc.edu } } } [128.174.5.187]) } } } 14, 17 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } } } l6CHh8Kh028959 } } } 14, 17 -- for {microscopy-at-microscopy.com} ; Thu, } 12 Jul 2007 12:43:08 -0500 } } } 14, 17 -- Received: from redkite.cvm.uiuc.edu } (redkite.cvm.uiuc.edu } } } [130.126.18.157]) } } } 14, 17 -- by expredir4.cites.uiuc.edu } (8.14.1/8.14.1) with ESMTP id } } } l6CHh8Mf003938 } } } 14, 17 -- for {microscopy-at-microscopy.com} ; Thu, } 12 Jul 2007 12:43:08 -0500 } } } (CDT) } } } 14, 17 -- Message-ID: {4696682D.20101-at-uiuc.edu} } } } 14, 17 -- Date: Thu, 12 Jul 2007 12:43:09 -0500 } } } 14, 17 -- From: Lou Ann Miller } {lamiller-at-uiuc.edu} } } } 14, 17 -- Reply-To: lamiller-at-uiuc.edu } } } 14, 17 -- User-Agent: Thunderbird 2.0.0.4 } (Macintosh/20070604) } } } 14, 17 -- MIME-Version: 1.0 } } } 14, 17 -- To: microscopy-at-microscopy.com } } } 14, 17 -- Subject: Taking the gluteraldehyde out } } } 14, 17 -- Content-Type: text/plain; } charset=ISO-8859-1; format=flowed } } } 14, 17 -- Content-Transfer-Encoding: 7bit } } } ==============================End of - } Headers============================== } } } } } } } } } } } -- } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Lou Ann Miller, MT(ASCP) } Service Supervisor } Center for Microscopic Imaging } College of Veterinary Medicine } Rm 1204 VMBSB } 2001 S Lincoln Ave } Urbana, IL 61802 } } 217/244-1567 } http://treefrog.cvm.uiuc.edu } } } ==============================Original } Headers============================== } 9, 19 -- From lamiller-at-uiuc.edu Thu Jul 12 13:04:40 } 2007 } 9, 19 -- Received: from expredir4.cites.uiuc.edu } (expredir4.cites.uiuc.edu [128.174.5.187]) } 9, 19 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l6CI4djI008640 } 9, 19 -- for {microscopy-at-microscopy.com} ; Thu, 12 } Jul 2007 13:04:40 -0500 } 9, 19 -- Received: from redkite.cvm.uiuc.edu } (redkite.cvm.uiuc.edu [130.126.18.157]) } 9, 19 -- by expredir4.cites.uiuc.edu } (8.14.1/8.14.1) with ESMTP id l6CI4dFs011549; } 9, 19 -- Thu, 12 Jul 2007 13:04:39 -0500 (CDT) } 9, 19 -- Message-ID: {46966D38.1090401-at-uiuc.edu} } 9, 19 -- Date: Thu, 12 Jul 2007 13:04:40 -0500 } 9, 19 -- From: Lou Ann Miller {lamiller-at-uiuc.edu} } 9, 19 -- Reply-To: lamiller-at-uiuc.edu } 9, 19 -- User-Agent: Thunderbird 2.0.0.4 } (Macintosh/20070604) } 9, 19 -- MIME-Version: 1.0 } 9, 19 -- To: "Webster, Paul" {PWebster-at-hei.org} , } microscopy-at-microscopy.com } 9, 19 -- Subject: Re: [Microscopy] Taking the } gluteraldehyde out } === message truncated ===
____________________________________________________________________________________ Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. http://answers.yahoo.com/dir/?link=list&sid=396545469
Dear Jon- It is an issue for all of us. I have a grant pending at the NIH for a new TEM to replace my "baby", which was installed in 1982. Even with really good projects, I'm on pins-and-needles waiting for our score. The only other ways I've heard for people to get major instruments (EMs, confocals, etc) without submitting shared instrumentation grant applications were pooling of funds by a group of investigators who then share the instrument exclusively, or being fortunate enough to have generous private donations to your institution earmarked for a specific piece of equipment. For the latter, you need a really strong fund-raising group and/or a core group of generous people of means seeking meaningful ways to get tax deductions ;-). In the meantime, try asking around at other institutions in your region. Someone may have an ESEM with time available. Our local orthopedic hospital (which has a research department) has one. I will be interesting to read any other advice you receive about alternate funding sources. Good luck, Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Weill Cornell Medical College
I use the Megaview III from SIS with most satisfaction. The support I received from this company is not common (in terms of speed and efficiency) and it is a pleasure for me to promote it if I can. No other interest in this company other than to make other customers happy ;-)
Stephane
--- anita.garg-at-grc.nasa.gov wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the } Microscopy Listserver } using the WWW based Form at } http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a } Subscriber, so when replying } please copy both anita.garg-at-grc.nasa.gov as well } as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: anita.garg-at-grc.nasa.gov } Name: Anita Garg } } Organization: NASA GRC } } Title-Subject: [Filtered] Veleta: TEM CCD camera?? } } Question: We are planning to buy a side mounted CCD } camera for our CM200 TEM. We are considering the "2k } X 2k Veleta" from Olympus SIS. We would like to hear } the comments from the experienced users. Any } suggestions regarding the "Diffraction Software" } would be highly appreciated too. } TIA } Anita } } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Thu Jul 12 } 21:55:29 2007 } 6, 11 -- Received: from [206.69.208.22] } (mac22.zaluzec.com [206.69.208.22]) } 6, 11 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l6D2tSnW003926 } 6, 11 -- for {microscopy-at-microscopy.com} ; Thu, 12 } Jul 2007 21:55:29 -0500 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: } {p06240802c2bc9a103487-at-[206.69.208.22]} } 6, 11 -- Date: Thu, 12 Jul 2007 21:55:27 -0500 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: anita.garg-at-grc.nasa.gov (by way of } MicroscopyListserver) } 6, 11 -- Subject: viaWWW: Veleta: TEM CCD camera?? } 6, 11 -- Content-Type: text/plain; } charset="us-ascii" } ==============================End of - } Headers============================== }
____________________________________________________________________________________ Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. http://surveylink.yahoo.com/gmrs/yahoo_panel_invite.asp?a=7
I need information on confocal systems-pricing, accessories, etc. Vendors may contact me offline at my gov address. Users may contact me either off or online. Thank you for your assistance.
Bruce F. Ingber, Biologist Electron Microscopy USDA-ARS, SRRC CSQ-EM 1100 Robert E. Lee Blvd. New Orleans, LA 70124 bingber-at-srrc.ars.usda.gov bingber46-at-hotmail.com 504-286-4270 desk phone 504-782-6323 cell
==============================Original Headers============================== 4, 20 -- From bingber-at-srrc.ars.usda.gov Fri Jul 13 09:53:19 2007 4, 20 -- Received: from srrc.ars.usda.gov (marconi.srrc.ars.usda.gov [199.133.86.11]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6DErH6U024493 4, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 13 Jul 2007 09:53:18 -0500 4, 20 -- Received: from (unknown [199.133.86.11]) by DA32USMOKC1_AVS01.usda.gov with smtp 4, 20 -- id 7b12_ca8cd294_3150_11dc_b13d_001143d22ff1; 4, 20 -- Fri, 13 Jul 2007 14:53:18 +0000 4, 20 -- Received: from SRRCDOM-MTA by srrc.ars.usda.gov 4, 20 -- with Novell_GroupWise; Fri, 13 Jul 2007 09:53:14 -0500 4, 20 -- Message-Id: {s6974b8a.010-at-srrc.ars.usda.gov} 4, 20 -- X-Mailer: Novell GroupWise Internet Agent 6.0.4 4, 20 -- Date: Fri, 13 Jul 2007 09:53:05 -0500 4, 20 -- From: "Bruce Ingber" {bingber-at-srrc.ars.usda.gov} 4, 20 -- To: {Microscopy-at-MSA.Microscopy.Com} 4, 20 -- Subject: Information on Confocal Microscopy Systems 4, 20 -- Mime-Version: 1.0 4, 20 -- Content-Type: text/plain; charset=US-ASCII 4, 20 -- Content-Disposition: inline 4, 20 -- Content-Transfer-Encoding: 8bit 4, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l6DErH6U024493 ==============================End of - Headers==============================
Thank you for the software suggestions (also Image Arithmetic).
I think the biggest problems are the reflections and an aperture that doesn't match the rest of the optics. Perhaps it's a simple parts mismatch. I doubt I'm being too picky expecting a high end research microscope to have even illumination edge-to-edge.
-Michael
At 11:34 AM 07/13/07, you wrote: } I thought I would mention it in case you weren't familiar with it. In } the Axiovison software (V4.?) there is a software button you can enable } called "shading correction" } It appears to even out the image illumination. } I have heard rumors in the lab that it is sometimes used instead of } properly aligning the microscope: )
____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. URL: http://www.aecom.yu.edu/aif/
==============================Original Headers============================== 6, 31 -- From cammer-at-aecom.yu.edu Fri Jul 13 12:18:12 2007 6, 31 -- Received: from mx1.aecom.yu.edu (mx1.aecom.yu.edu [129.98.1.51]) 6, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6DHICh7007300 6, 31 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jul 2007 12:18:12 -0500 6, 31 -- Received: from draco.aecom.yu.edu (draco.aecom.yu.edu [129.98.1.160]) 6, 31 -- by mx1.aecom.yu.edu (Postfix) with ESMTP id 111249F0116 6, 31 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jul 2007 13:18:12 -0400 (EDT) 6, 31 -- Received: from draco.aecom.yu.edu (unknown [127.0.0.1]) 6, 31 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 89B378B4002 6, 31 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jul 2007 13:09:01 -0400 (EDT) 6, 31 -- X-AuditID: 816201a0-9df25bb000005b7e-c2-4697b1adaa95 6, 31 -- Received: from post.aecom.yu.edu (post.aecom.yu.edu [129.98.1.100]) 6, 31 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 6144B718002 6, 31 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jul 2007 13:09:01 -0400 (EDT) 6, 31 -- Received: from AIF3.aecom.yu.edu (aif3.aif.aecom.yu.edu [129.98.80.70]) 6, 31 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 6, 31 -- (No client certificate requested) 6, 31 -- by post.aecom.yu.edu (Postfix) with ESMTP id A658539 6, 31 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jul 2007 13:18:11 -0400 (EDT) 6, 31 -- Message-Id: {7.0.1.0.2.20070713131334.04f406c0-at-aecom.yu.edu} 6, 31 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 6, 31 -- Date: Fri, 13 Jul 2007 13:18:11 -0400 6, 31 -- To: microscopy-at-microscopy.com 6, 31 -- From: Michael Cammer {cammer-at-aecom.yu.edu} 6, 31 -- Subject: Re: [Microscopy] Zeiss Axioskop II uneven illumination 6, 31 -- In-Reply-To: {f9156846be0c61661916100caf4c9ad6-at-mit.edu} 6, 31 -- References: {200707122127.l6CLRavJ011203-at-ns.microscopy.com} 6, 31 -- {f9156846be0c61661916100caf4c9ad6-at-mit.edu} 6, 31 -- Mime-Version: 1.0 6, 31 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 31 -- X-Brightmail-Tracker: AAAAAA== ==============================End of - Headers==============================
I work mostly with plastic resin embedded tissue and only sporadically with paraffin. Is there a rule of thumb for how many slides can be dewaxed per volume before it is necessary to switch solutions. I understand the concept of moving Xylene bath 3 to bath 2 and bath 2 to bath 1 and fresh into bath 3 but at what point? Thanks, Tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
On the automatic Leica stainers used (and in the manual procedures set up in our SOPs) the magic number was 400 slides, volume of each station approx 450ml, 3x xylene, 2x 100% EtOH, 2x 95% EtOH. Hope that helps.
Roger Moretz, Ph.D. Principally from nowhere, retired from the grind as of July 4th but not dead yet.
On 7/13/07, phillipst-at-missouri.edu {phillipst-at-missouri.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I work mostly with plastic resin embedded tissue and only sporadically with } paraffin. Is there a rule of thumb for how many slides can be dewaxed per } volume before it is necessary to switch solutions. I understand the concept } of moving Xylene bath 3 to bath 2 and bath 2 to bath 1 and fresh into bath } 3 but at what point? Thanks, Tom } } } } Thomas E. Phillips, PhD } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } } 573-882-4712 (office) } 573-882-0123 (fax) } PhillipsT-at-missouri.edu } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } } ==============================Original Headers============================== } 6, 18 -- From PhillipsT-at-missouri.edu Fri Jul 13 15:15:07 2007 } 6, 18 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) } 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6DKF7O5026806 } 6, 18 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 13 Jul 2007 15:15:07 -0500 } 6, 18 -- Received: from um-tsmtpout1.um.umsystem.edu ([209.106.228.23]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } 6, 18 -- Fri, 13 Jul 2007 15:15:07 -0500 } 6, 18 -- Received: from phillips-dell.missouri.edu ([128.206.81.132]) by um-tsmtpout1.um.umsystem.edu over TLS secured channel with Microsoft SMTPSVC(6.0.3790.3959); } 6, 18 -- Fri, 13 Jul 2007 15:15:07 -0500 } 6, 18 -- Message-Id: {6.0.0.22.2.20070713151059.04047110-at-pop.missouri.edu} } 6, 18 -- X-Sender: phillipst-at-pop.missouri.edu } 6, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 6.0.0.22 } 6, 18 -- Date: Fri, 13 Jul 2007 15:13:01 -0500 } 6, 18 -- To: Microscopy-at-msa.microscopy.com } 6, 18 -- From: "Thomas E. Phillips" {phillipst-at-missouri.edu} } 6, 18 -- Subject: paraffin dewaxing } 6, 18 -- Mime-Version: 1.0 } 6, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } 6, 18 -- X-OriginalArrivalTime: 13 Jul 2007 20:15:07.0098 (UTC) FILETIME=[816E0BA0:01C7C58A] } ==============================End of - Headers============================== }
In our lab, we change the solutions every 300, maximum 400 slides (500 ml) or once per week, depending on which limit is reached first.
Sven Terclavers
-----Original Message----- X-from: phillipst-at-missouri.edu [mailto:phillipst-at-missouri.edu] Sent: Friday, July 13, 2007 10:18 PM To: sven.terclavers-at-med.kuleuven.be
I work mostly with plastic resin embedded tissue and only sporadically with paraffin. Is there a rule of thumb for how many slides can be dewaxed per volume before it is necessary to switch solutions. I understand the concept of moving Xylene bath 3 to bath 2 and bath 2 to bath 1 and fresh into bath 3 but at what point? Thanks, Tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
I have an ISI/Topcon SX30 microscope with no manuals/schematics. If anyone has a set of manuals/schematics as pdfs or as paper versions I'd appreciate a copy. Please contact me.
Thank you
Lee Levine Process Solutions Consulting 8009 George Road, New Tripoli, PA 18066 mobile 610-248-2002, fax 610-285-4575 email levilr-at-hughes.net
==============================Original Headers============================== 4, 18 -- From levilr-at-hughes.net Sat Jul 14 18:07:43 2007 4, 18 -- Received: from n016.sc0.he.tucows.com (smtpout1073.sc0.he.tucows.com [64.97.144.73]) 4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6EN7hlm009061 4, 18 -- for {Microscopy-at-Microscopy.Com} ; Sat, 14 Jul 2007 18:07:43 -0500 4, 18 -- Received: from Laptop (12.109.43.95) by n016.sc0.he.tucows.com (7.2.078) (authenticated as levilr-at-hughes.net) 4, 18 -- id 46984B780000D2A6 for Microscopy-at-Microscopy.Com; Sat, 14 Jul 2007 23:07:42 +0000 4, 18 -- Message-ID: {46984B780000D2A6-at-n016.sc0.he.tucows.com} (added by postmaster-at-bouncemessage.net) 4, 18 -- From: "Lee Levine" {levilr-at-hughes.net} 4, 18 -- To: {Microscopy-at-Microscopy.Com} 4, 18 -- Subject: Manuals for ISI/Topcon SX30 4, 18 -- Date: Sat, 14 Jul 2007 19:07:40 -0400 4, 18 -- MIME-Version: 1.0 4, 18 -- Content-Type: text/plain; 4, 18 -- charset="us-ascii" 4, 18 -- Content-Transfer-Encoding: 7bit 4, 18 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353 4, 18 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 4, 18 -- Thread-Index: AcfGa8Z+1f3l7fg4QAGABfERRImTnQ== ==============================End of - Headers==============================
On Jul 12, 2007, at 7:57 PM, cvierret-at-umr.edu wrote:
} I have been charged with measuring the beam diameter in our scopes. I } have researched some methods and most involve a Si knife. Do any of } you have a method that you have used to measure beam diameter and if } so where did you obtain your materials. Any and all information would } be appreciated. } Dear Clarissa, When I did this, I exposed a piece of film at a high enough mag that the maximum intensity did not saturate the film, and the beam still fit onto the film. I then scanned the film and got a measure of intensity vs. distance from the beam center. I was interested in determining the minimum probe size I could get, which happened at the highest Wehnelt bias and first condenser lens settings, so the intensity was low enough to do this. You could try the same thing with a CCD by first going to a high enough mag so that the CCD was not saturated, then taking other images at lower mags, if necessary, to include all the beam. You might have to use the beam stop to intercept the brightest part of the beam at these lower mags. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Sat Jul 14 18:41:19 2007 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6ENfIxE021233 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Sat, 14 Jul 2007 18:41:18 -0500 4, 22 -- Received: from fire-dog.its.caltech.edu (fire-dog [192.168.1.4]) 4, 22 -- by wood-ox-postvirus (Postfix) with ESMTP id E5CDE12E18; 4, 22 -- Sat, 14 Jul 2007 16:41:12 -0700 (PDT) 4, 22 -- Received: from [192.168.159.233] (jpix-01.caltech.edu [131.215.2.133]) 4, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id 6DCDF12E9A; 4, 22 -- Sat, 14 Jul 2007 16:41:11 -0700 (PDT) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v624) 4, 22 -- In-Reply-To: {200707130257.l6D2v49p005066-at-ns.microscopy.com} 4, 22 -- References: {200707130257.l6D2v49p005066-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {64ed397d8e23d1d12209f561e9092177-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] viaWWW: Measuring Beam Diameter 4, 22 -- Date: Sat, 14 Jul 2007 16:54:58 -0700 4, 22 -- To: microscopy-at-msa.microscopy.com, cvierret-at-umr.edu 4, 22 -- X-Mailer: Apple Mail (2.624) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.4.5 ==============================End of - Headers==============================
Bitplane is expanding its US team and asks for applications for the following two positions. Feel free to forward this e-mail to anyone who might be interested.
Position #1 - Central and South Eastern Region Sales Representative US / Canada
Bitplane is looking for a biologist with strong computer skills and at least one year of hands-on experience using a confocal or similar 3D advanced light microscope. The duties of this position include:
. Customer visits and analysis of customer's imaging needs. . Demonstration of the software and onsite work with the customer . Organization of exhibitions and workshops . Sales support of existing customers
The candidate is expected to have an outgoing personality with strong communication skills and should look forward to increased responsibility. At least 50% travel will be required. Representative is required to live in the territory they cover. Benefits include a base salary, performance based commission, 401K plan, healthcare, and vacation. Representative will work out of a home office. We offer a team of 18 people, fun to work with, and a truly international environment that provides the resources required to grow. We don't mind if the candidate does not have much business experience and we are prepared to show him/her the sales skills at the job.
Position #2 - Sales Development Coordinator
Bitplane is looking for a candidate with a science background and knowledge of the microscope community. The duties of this position include
. Identifying potential new regional customers via web searches, literature searches, marketing campaigns, trade shows, and customer referrals. . Introduction of Bitplane products and services to potential customers via email, phone, and Webex. . Understanding potential customers needs related to products offered by Bitplane. . Organizing workshops and demonstrations for regional sales representatives
The candidate is expected to have an outgoing personality, is detail oriented, articulates clearly, exhibits a high level of professionalism, and exceptional organization.Travel is not required. Representative is/will be based in the Minneapolis / Saint Paul area of MN. Benefits include a base salary, 401K plan, healthcare, and vacation. Representatives will work out of a home office and travel is not required on routine basis. We offer a team of 18 people, fun to work with, and a truly international environment that provides the resources required to grow.
Applicants should reply with a letter stating what position they are interested in, why they are qualified for the position, and enclose their CV.
With best regards, Mike
Bitplane Inc. Michael C. Wussow Vice President and General Manager Bitplane Inc.
Cell Phone: 651-336-4600 Fax: 866-691-9112 Toll Free: 1-888-3D-BITPX (332-4879) Visit Our Web Site At: www.bitplane.com
==============================Original Headers============================== 16, 31 -- From mike-at-bitplane.com Mon Jul 16 10:30:59 2007 16, 31 -- Received: from host55a.simplicato.com (host55a.simplicato.com [207.99.47.55]) 16, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6GFUx7A000357 16, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jul 2007 10:30:59 -0500 16, 31 -- Received: from localhost (localhost.simplicato.com [127.0.0.1]) 16, 31 -- by host55a.simplicato.com (Postfix) with ESMTP id 7DCEBB2165E 16, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jul 2007 11:30:59 -0400 (EDT) 16, 31 -- Received: from host55a.simplicato.com ([127.0.0.1]) 16, 31 -- by localhost (host55a.simplicato.com [127.0.0.1]) (amavisd-new, port 10024) 16, 31 -- with ESMTP id 13533-06 for {Microscopy-at-microscopy.com} ; 16, 31 -- Mon, 16 Jul 2007 11:30:59 -0400 (EDT) 16, 31 -- Received: from MikeLaptop (71-34-23-82.mpls.qwest.net [71.34.23.82]) 16, 31 -- by host55a.simplicato.com (Postfix) with ESMTP id 3ED89B2164D 16, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jul 2007 11:30:57 -0400 (EDT) 16, 31 -- Reply-To: {mike-at-bitplane.com} 16, 31 -- From: "Michael C. Wussow" {mike-at-bitplane.com} 16, 31 -- To: {Microscopy-at-microscopy.com} 16, 31 -- Subject: Positions Available in the USA 16, 31 -- Date: Mon, 16 Jul 2007 10:30:51 -0500 16, 31 -- Organization: Bitplane Inc. 16, 31 -- Message-ID: {03b601c7c7be$4db9bbc0$e92d3340$-at-com} 16, 31 -- MIME-Version: 1.0 16, 31 -- Content-Type: text/plain; 16, 31 -- charset="us-ascii" 16, 31 -- Content-Transfer-Encoding: 7bit 16, 31 -- X-Mailer: Microsoft Office Outlook 12.0 16, 31 -- Thread-Index: AcfHvkkKCHFvr+HdSTyBHQ8AGFgmMw== 16, 31 -- Content-Language: en-us 16, 31 -- x-cr-hashedpuzzle: AHiL AOVR A5N/ CAZM CC1d C2Lp E/is FD+5 Frwz GUJC HHFx IZJv KMmf KY0v KnTC Kqm3;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{4B373F78-0513-481E-896A-C64188D5A6B0};bQBpAGsAZQBAAGIAaQB0AHAAbABhAG4AZQAuAGMAbwBtAA==;Mon, 16 Jul 2007 15:30:48 GMT;UABvAHMAaQB0AGkAbwBuAHMAIABBAHYAYQBpAGwAYQBiAGwAZQAgAGkAbgAgAHQAaABlACAAVQBTAEEA 16, 31 -- x-cr-puzzleid: {4B373F78-0513-481E-896A-C64188D5A6B0} 16, 31 -- X-Virus-Scanned: by amavisd-new at simplicato.com ==============================End of - Headers==============================
I could use either a paper or a pdf copy of the manual for a Gatan 791 CCD camera for a TEM that includes installation instructions. Or, specific advice on the air line connection and pressure for a JEOL 1200.
Thanks in advance!
Robert
Robert Fitton Teaching Associate/Director of Labs Luther College Department of Biology 700 College Drive Decorah, IA 52101 563-387-1559 Voice 563-387-1080 FAX fittonro-at-luther.edu
==============================Original Headers============================== 10, 29 -- From fittonro-at-luther.edu Mon Jul 16 14:15:50 2007 10, 29 -- Received: from antispam.luther.edu (antispam.luther.edu [192.203.196.14]) 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6GJFoAP018260 10, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Jul 2007 14:15:50 -0500 10, 29 -- X-ASG-Debug-ID: 1184613350-675e00080000-JFxeax 10, 29 -- X-Barracuda-URL: http://antispam.luther.edu:80/cgi-bin/mark.cgi 10, 29 -- X-Barracuda-Connect: smtp-4.luther.edu[192.203.196.22] 10, 29 -- X-Barracuda-Start-Time: 1184613350 10, 29 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 10, 29 -- X-ASG-Whitelist: Client 10, 29 -- Received: from smtp-4.luther.edu (smtp-4.luther.edu [192.203.196.22]) 10, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 10, 29 -- (No client certificate requested) 10, 29 -- by antispam.luther.edu (Spam Firewall) with ESMTP id 5D4A0426728 10, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Jul 2007 14:15:50 -0500 (CDT) 10, 29 -- Received: from [172.17.16.49] (host-17-16-49.internal.luther.edu [172.17.16.49]) 10, 29 -- by smtp-4.luther.edu (8.13.6/8.13.6/SuSE Linux 0.8) with ESMTP id l6GJFnCT010171 10, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Jul 2007 14:15:50 -0500 10, 29 -- Mime-Version: 1.0 (Apple Message framework v752.2) 10, 29 -- Content-Transfer-Encoding: 7bit 10, 29 -- Message-Id: {303B4D30-DCB9-4096-B41B-D1525C65E27D-at-luther.edu} 10, 29 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 10, 29 -- To: Microscopy-at-Microscopy.Com 10, 29 -- From: Robert Fitton {fittonro-at-luther.edu} 10, 29 -- X-ASG-Orig-Subj: Gatan 791 manual 10, 29 -- Subject: Gatan 791 manual 10, 29 -- Date: Mon, 16 Jul 2007 14:11:48 -0500 10, 29 -- X-Mailer: Apple Mail (2.752.2) 10, 29 -- X-Barracuda-Virus-Scanned: by Luther College Antispam Service at luther.edu ==============================End of - Headers==============================
Here's kind of a dumb question. How do you all measure out embedding resin catalyst? I've only been doing it for 31 years... I used to use tuberculin syringes - had to hurry because they sort of melt. The current crop of facility users seem dumbfounded that I would use anything but a pipetter. But I have now had another new pipetter get gummed up with some resin component, probably catalyst. Does anyone have a favorite method???
Aloha with exasperation, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 5, 19 -- From tina-at-pbrc.hawaii.edu Mon Jul 16 19:41:40 2007 5, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6H0fcsx005560 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 16 Jul 2007 19:41:39 -0500 5, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id l6H0fZfB008820 5, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 16 Jul 2007 14:41:36 -1000 (HST) 5, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id l6H0fY0E008817 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 16 Jul 2007 14:41:35 -1000 (HST) 5, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 5, 19 -- Date: Mon, 16 Jul 2007 14:41:33 -1000 (HST) 5, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 19 -- X-Sender: tina-at-halia 5, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 5, 19 -- Subject: pipeting resin components 5, 19 -- Message-ID: {Pine.GSO.4.21.0707161437130.8234-100000-at-halia} 5, 19 -- MIME-Version: 1.0 5, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
Ah yes, I remember those days of plugged pipetters!
To eliminate this we weigh all resin components into a dispo beaker. We can get close enough with a decent 3 decimal place scale to get consistent results and use only disposable pipettes for all components.
Our scale sits in a hood so is protected by a draft shield. This has gotten rather messy over the years as students occasionally use acetone on the polyethylene shield to clean up spills........but still works. A piece of aluminum foil on the pan has worked to prevent resin from getting onto scale components that would affect function.
Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907
On 7/16/07 8:45 PM, "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, all- } } Here's kind of a dumb question. How do you all measure out embedding resin } catalyst? I've only been doing it for 31 years... I used to use tuberculin } syringes - had to hurry because they sort of melt. The current crop of } facility users seem dumbfounded that I would use anything but a } pipetter. But I have now had another new pipetter get gummed up with some } resin component, probably catalyst. Does anyone have a favorite method??? } } Aloha with exasperation, } Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } **************************************************************************** } } } ==============================Original Headers============================== } 5, 19 -- From tina-at-pbrc.hawaii.edu Mon Jul 16 19:41:40 2007 } 5, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu } [128.171.22.7]) } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l6H0fcsx005560 } 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 16 Jul 2007 19:41:39 -0500 } 5, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) } 5, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id } l6H0fZfB008820 } 5, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) } 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 16 Jul 2007 14:41:36 -1000 } (HST) } 5, 19 -- Received: from localhost by halia.pbrc.hawaii.edu } (8.12.11/8.12.11/Submit) with ESMTP id l6H0fY0E008817 } 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 16 Jul 2007 14:41:35 -1000 } (HST) } 5, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process } doing -bs } 5, 19 -- Date: Mon, 16 Jul 2007 14:41:33 -1000 (HST) } 5, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} } 5, 19 -- X-Sender: tina-at-halia } 5, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} } 5, 19 -- Subject: pipeting resin components } 5, 19 -- Message-ID: {Pine.GSO.4.21.0707161437130.8234-100000-at-halia} } 5, 19 -- MIME-Version: 1.0 } 5, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII } ==============================End of - Headers==============================
==============================Original Headers============================== 11, 23 -- From dsherman-at-purdue.edu Mon Jul 16 20:03:19 2007 11, 23 -- Received: from 1061exfe02.adpc.purdue.edu (1061exfe02.adpc.purdue.edu [128.210.63.223]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6H13JJ1017576 11, 23 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jul 2007 20:03:19 -0500 11, 23 -- Received: from exchange.purdue.edu ([172.21.6.23]) by 1061exfe02.adpc.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 11, 23 -- Mon, 16 Jul 2007 21:03:19 -0400 11, 23 -- Received: from 74.140.44.52 ([74.140.44.52]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 11, 23 -- Tue, 17 Jul 2007 01:03:19 +0000 11, 23 -- User-Agent: Microsoft-Entourage/11.3.3.061214 11, 23 -- Date: Mon, 16 Jul 2007 21:03:18 -0400 11, 23 -- Subject: Re: [Microscopy] pipeting resin components 11, 23 -- From: Debby Sherman {dsherman-at-purdue.edu} 11, 23 -- To: Tina Carvalho {tina-at-pbrc.hawaii.edu} , 11, 23 -- "message to: MSA list" {microscopy-at-microscopy.com} 11, 23 -- Message-ID: {C2C18D96.F582%dsherman-at-purdue.edu} 11, 23 -- Thread-Topic: [Microscopy] pipeting resin components 11, 23 -- Thread-Index: AcfIDkLZgbBrLDQBEdy0cQAKlcoUxg== 11, 23 -- In-Reply-To: {200707170045.l6H0jZ3l009186-at-ns.microscopy.com} 11, 23 -- Mime-version: 1.0 11, 23 -- Content-type: text/plain; 11, 23 -- charset="US-ASCII" 11, 23 -- Content-transfer-encoding: 7bit 11, 23 -- X-OriginalArrivalTime: 17 Jul 2007 01:03:19.0539 (UTC) FILETIME=[43C46430:01C7C80E] ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both shah0211-at-umn.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: shah0211-at-umn.edu Name: Neha Shah
Organization: U of Minnesota
Title-Subject: [Filtered] Diamond knife
Question: Our lab is looking to buy a diamond knife and we are debating between PELCO and DiaTome. Our EM facility currently uses DiaTome and it works well. Does anyone have information about PELCO diamond knife and whether they work well with biological samples?
} Tina, } } Ah yes, I remember those days of plugged pipetters! } } To eliminate this we weigh all resin components into } a dispo beaker. We can } get close enough with a decent 3 decimal place scale } to get consistent } results and use only disposable pipettes for all } components.
____________________________________________________________________________________ Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. http://answers.yahoo.com/dir/?link=list&sid=396545433
Glass transfer pipettes and rubber bulbs. Measure by weight.
Phil
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
I wondered where is the development of this technique. It obviously uses supraconductivity, so it needs temperatures lower than LN2, how is this temperature maintained? Is anyone in the field, or have had the chance to use this technology?
Best regards,
Stephane
____________________________________________________________________________________ TV dinner still cooling? Check out "Tonight's Picks" on Yahoo! TV. http://tv.yahoo.com/
Aloha, Tina- I use a pipettor, but I use the big ones (1000microliters) to measure out 300-600 microliters of catalyst so that I don't risk pulling the gooey stuff up into the works. If I need to make a large volume, I measure out the catalyst in 2 shots. Simple, but fairly effective. I haven't had a gummed-up pipettor in a while. (Now watch, it will happen this week!) Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Weill Cornell Medical College
This Question was submitted to Ask-A-Microscopist by (fli-at-estee.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, July 17, 2007 at 08:08:29 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both fli-at-estee.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: fli-at-estee.com Name: Foo Li
Organization: Estee Lauder
Education: Graduate College
Location: Melville, NY 11747
Title: FEI Phenom Electron-light microscope
Question: Is the Phenom the real deal? 30 nm resolution is not all that bad especially when dealing with emulsions. I need an opinion of this product from actual Phenom users out there.
Trying to weigh the catalyst in the fume hood, I have a problem of air flow of the hood disturbing the balance reading. I once held my nose (away from the hood) and measured the number of drops of catalyst from a particular smallish PE disposable pipet required to make the desired weight. It is very repeatable if you let the drops form (don't squirt) and use the same style pipet. I often mix resin batches in the 50cc range that require 0.2g of catalyst. I weigh the main components out in the lab (scissor off the tip of larger dispo plastic pipet for the viscous resins), mix well, then move to the hood and add the drops of catalyst. For my pipets and DMP-30 or DMAE I use 12 drops for 0.2g.
Since Spurr's resin works fine with half the normal catalyst for "Long Pot Life" mix, the tiny variations in the drop method certainly will have very little effect on the final product.
Cheers,
Dale
lcgould-at-med.cornell.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Aloha, Tina- } I use a pipettor, but I use the big ones (1000microliters) to } measure out 300-600 microliters of catalyst so that I don't risk } pulling the gooey stuff up into the works. If I need to make a large } volume, I measure out the catalyst in 2 shots. Simple, but fairly } effective. I haven't had a gummed-up pipettor in a while. (Now } watch, it will happen this week!) } Lee
Stephane, We've been working with one of the earliest microcalorimeters for about 5 years. They are beautiful spectrometers if you are willing to work around their shortcomings. Ours is capable of collecting energy dispersive spectra over energy ranges between a few hundred eV and 8 keV with resolutions of around 6 eV. Count rates are low - around one to two hundred counts per second. The energy dispersion is achieved by measuring the temperature increase in a block of normal (non-superconducting) metal. The thermometer is a superconductor held right at the temperature at which it makes the transition from normal conduction to superconduction. The change in resistance with change in temperature is large and can be read out using SQUIDS (superconducting quantum interference devices). The device is maintained at liquid He temperatures (~4 degrees) all the time and cooled to ~100 millikelvin using an adiabatic demagnetization refrigerator for operation. It can remain at 100 millikelvin for about 10 hours. To the best of my knowledge there are not currently any vendors selling microcalorimeters although there were in the past. There is at least one commercial unit in development. The cost is likely to be in the hundreds of thousands of dollar range per unit. Microcalorimeters are nothing like Si(Li) or SDD in terms of ease of use. Ours takes a couple of hours to cycle down to temperature and to lock the detector onto the edge of the superconducting transition. They require constant fiddling with half-a-dozen or more amplifiers and field coils. Someday they may be refined enough for routine analysis but today they remain laboratory experiments.
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi!
I wondered where is the development of this technique. It obviously uses supraconductivity, so it needs temperatures lower than LN2, how is this temperature maintained? Is anyone in the field, or have had the chance to use this technology?
A new short course "X06-Surface Analysis" is being offered at this years MM meeting in Ft. Lauderdale Florida (Sun, Aug 5, 2007) at 9am in room 222 of the convention center. We have been informed that space is still available. This is an excellent opportunity to learning more about state of the art surface analysis instrumentation through examples.
Course summary: For many biological and materials systems, the properties of the specimen's outer surface dictate its performance. Surface analysts are being asked to detect specimen components present in ever lower concentrations and within smaller spatial dimensions. This short course will describe and contrast the main surface analytical instruments: Auger Electron Spectroscopy (AES), X-ray Photoelectron Spectroscopy (XPS), Secondary Ion Mass Spectrometry (SIMS), and Scanning Probe Microscopy (SPM). Emphasis will be placed on the principles of each technique and on data interpretation. The limitations of each technique will be discussed. Upon completion of this short course, the attendee will be able to properly select which state-of-the-art surface analysis technique(s) should be used to address spectroscopy, imaging, and depth profiling analysis requests from their colleagues/customers.
Vincent S. Smentkowski GE Global Research 1 Research Circle Niskayuna, NY 12309 USA General Electric Company T 518 387 5467 F 518 387 6972 E smentkow-at-crd.ge.com
==============================Original Headers============================== 6, 29 -- From smentkow-at-crd.ge.com Tue Jul 17 11:35:36 2007 6, 29 -- Received: from ext-nj2ut-6.online-age.net (ext-nj2ut-6.online-age.net [64.14.54.235]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6HGZZDt014099 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Jul 2007 11:35:36 -0500 6, 29 -- Received: from int-nj2ut-2.online-age.net (int-nj2ut-2.online-age.net [3.159.237.71]) 6, 29 -- by ext-nj2ut-6.online-age.net (8.13.6/8.13.6/20051114-SVVS-TLS-DNSBL) with ESMTP id l6HGZYdm001279 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Jul 2007 12:35:34 -0400 6, 29 -- Received: from cinmlef06.e2k.ad.ge.com (int-nj2ut-2.online-age.net [3.159.237.71]) 6, 29 -- by int-nj2ut-2.online-age.net (8.13.6/8.13.6/20050510-SVVS) with ESMTP id l6HGZW2Y018547 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Jul 2007 12:35:33 -0400 6, 29 -- Received: from CINMLVEM12.e2k.ad.ge.com ([3.159.214.56]) by cinmlef06.e2k.ad.ge.com with Microsoft SMTPSVC(6.0.3790.2499); 6, 29 -- Tue, 17 Jul 2007 12:35:32 -0400 6, 29 -- x-mimeole: Produced By Microsoft Exchange V6.5 6, 29 -- Content-class: urn:content-classes:message 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Type: text/plain; 6, 29 -- charset="US-ASCII" 6, 29 -- Subject: new short course at MM2007 - space still available 6, 29 -- Date: Tue, 17 Jul 2007 12:35:29 -0400 6, 29 -- Message-ID: {BAAA0A225D59F244A767AB0C9D15384101B2E708-at-CINMLVEM12.e2k.ad.ge.com} 6, 29 -- X-MS-Has-Attach: 6, 29 -- X-MS-TNEF-Correlator: 6, 29 -- Thread-Topic: new short course at MM2007 - space still available 6, 29 -- Thread-Index: AcfIkHzJ9rFqh6nvQqS4u6yGoQZXCw== 6, 29 -- From: "Smentkowski, Vincent S (GE, Research)" {smentkow-at-crd.ge.com} 6, 29 -- To: {Microscopy-at-Microscopy.Com} 6, 29 -- X-OriginalArrivalTime: 17 Jul 2007 16:35:32.0279 (UTC) FILETIME=[7E473470:01C7C890] 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l6HGZZDt014099 ==============================End of - Headers==============================
The Pelco and Diatome are both high quality knives which should work very well with biological samples.
Having said that, we feel the Drukker knife is the best value and quality knife on the market. You might want to check out the UL3 for the best in both quality and value.
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
I had to giggle at the many replies to my cry of frustration about measuring catalyst for resin! Like Tamara, over the years I have become adept at sequentially pouring the resin components into a tri-cornered beaker, stirring magnetically, then adding catalyst. Many years ago I used to measure out the components with syringes and then laboriously clean the syringes and keep them for the next use. Once I switched to weighing I kept using a tuberculin syringe to measure catalyst because my crappy balance couldn't weigh small amounts and did not do well in the fume hood; the draft shield was stolen years ago. Then I got mentally stuck. My syringes are desperately old, and the black rubber seal has been melting when in contact with the catalyst.
Now when I train people they look at me like I'm crazy when I pour, so I let them transfer components to the beaker on the balance with disposable plastic transfer pipets. I *do not allow* glass pipets! How many of you have sectioned glass chips?
The current crop of students don't seem to know how to use graduated pipets and two- or three-button bulbs, so I have been letting them use the pipetters to measure catalyst. They all know how to use pipetters, so it was an easy fix. In my cranky old age, though, I've been insisting they learn other ways to measure! I don't know how resin got up into the pipetters; I would have thought it was impossible, but it keeps happening.
I keep all wastes: transfer pipets, tips, syringes, and beakers, and polymerize them, thinking it makes it all less toxic when it goes to the landfill or waste-to-energy plant.
Tina's Tip: I put my molds and labels in the oven overnight to bake out, and now I never get bubbles!
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 8, 20 -- From tina-at-pbrc.hawaii.edu Tue Jul 17 16:53:40 2007 8, 20 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6HLrd7l013848 8, 20 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jul 2007 16:53:40 -0500 8, 20 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 8, 20 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id l6HLrZMI014584 8, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 8, 20 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jul 2007 11:53:36 -1000 (HST) 8, 20 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id l6HLrYCQ014581 8, 20 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jul 2007 11:53:35 -1000 (HST) 8, 20 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 8, 20 -- Date: Tue, 17 Jul 2007 11:53:34 -1000 (HST) 8, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 8, 20 -- X-Sender: tina-at-halia 8, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 8, 20 -- Subject: Re: [Microscopy] pipeting resin components 8, 20 -- In-Reply-To: {p06230919c2c27154801a-at-[140.251.48.23]} 8, 20 -- Message-ID: {Pine.GSO.4.21.0707171146180.13338-100000-at-halia} 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
It seems that in many ways my lab may be unique in its way of handling resin. Many years ago I threw out the concept of solutions A and B , and the WPE values and went instead to a method first proposed to me by Sus Ito at Harvard.
The method has worked consistently on three different continents and on a few EM course in different parts of the world. It consists of mixing three resin ingredients (but not the catalyst) in a large volume, aliquoting the mixture into small glass, screw-top vials and storing them, tightly closed, at -20 degrees for as long as it takes to finish the batch.
As resin is needed, a vial containing from 4 to 6 ml of resin is removed from the freezer and warmed in the oven. The actual amount of resin in the vial can be determined by comparing the resin level with a calibrated (and marked) black vial). It is important to know the amount of resin in the vail because this will determine the amount of catalyst to use.
Difficult to embed specimens can be soaked in the resin without catalyst for many days, or the resin can be mixed with catalyst and used immediately. The advantages are:
1. no messy resin preparation needed for each experiment. 2. no wasted resin components. 3. less handling of toxic components. 4. idiot-proof system ensuring reproducibility. 5. consistent results - the first vial is the same as the last vial. 6. no difficult measuring of catalyst (or any of the other components). 7. cleaner than other methods.
Want to know the method? Here it is:
Epon Resin (simplified recipe for making large batches of premixed resin)
Mix the following in a large glass jar (see below):
NMA 110 mL DDSA 130 mL Eponate 12 230 mL
When the ingredients have been well mixed, the resin is poured into small tubes in aliquots of 4 to 6 mL and stored frozen. For use, warm a tube and, using a glass pasteur pipette, add four drops of BDMA per milliliter (or 2 drops of DMP-30 if preferred). Mix well, use immediately and discard unused resin (polymerization is a good idea).
Preparing and storing resin this way will ensure that resin ingredients are not wasted and all the tubes will have resin of the same consistency. The mixture can be tested before use by polymerizing one tube of resin. All subsequent tubes of resin will have the same polymerization and sectioning qualities as this first tube.
Glass jar tip: A simple tip for measuring the resin components is to pre-calibrate a glass jar. In a clean jar, pour in 110mL of water, mark the meniscus. Add an extra 13mL of water and mark the new meniscus, and finally, add 230mL of water and mark the meniscus. (For those in the USA, Smuckers jars work well - UK, Robinson's orange marmalade - it just has to be clear glass!)
Pour out all the water and dry the jar well. It is now ready to be used for measuring out the resin components and mixing them. Mix the three components well before pouring the resin into the small vials. As the volume is estimated just prior to the resin being used, there is no need to get into complicated pipetting methods, just pour carefully.
As for glass pipettes (and I don't mean to be confrontational with the following comments), I have been using them for many years in my specimen preparation protocols. In addition to distributing catalyst, they are very useful for collecting scraped cells from culture dishes, and for transferring solutions from around very small specimens.
I am aware that glass fragments are supposed to enter embedded specimen blocks and damage diamond knives, but most people I know also trim their specimen blocks using glass knives, which is a more reasonable source of glass contamination.
In all the years I have been using glass pipettes I have never come across glass fragments in embedded tissues or cell pellets. I have seen hairs, dust and large chinks of keratin, but no glass. If glass were present in the blocks, I am sure it would be obvious in the initial trimming and facing off, which, incidentally we do with an old diamond knife.
I send this method out for all to try. If anyone is brave enough to try it, let me know if it was useful - I promise that it will change your life, especially is you are part of a multi-user facility.
Best regards,
Paul Webster.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
I had to giggle at the many replies to my cry of frustration about measuring catalyst for resin! Like Tamara, over the years I have become adept at sequentially pouring the resin components into a tri-cornered beaker, stirring magnetically, then adding catalyst. Many years ago I used to measure out the components with syringes and then laboriously clean the syringes and keep them for the next use. Once I switched to weighing I kept using a tuberculin syringe to measure catalyst because my crappy balance couldn't weigh small amounts and did not do well in the fume hood; the draft shield was stolen years ago. Then I got mentally stuck. My syringes are desperately old, and the black rubber seal has been melting when in contact with the catalyst.
Now when I train people they look at me like I'm crazy when I pour, so I let them transfer components to the beaker on the balance with disposable plastic transfer pipets. I *do not allow* glass pipets! How many of you have sectioned glass chips?
The current crop of students don't seem to know how to use graduated pipets and two- or three-button bulbs, so I have been letting them use the pipetters to measure catalyst. They all know how to use pipetters, so it was an easy fix. In my cranky old age, though, I've been insisting they learn other ways to measure! I don't know how resin got up into the pipetters; I would have thought it was impossible, but it keeps happening.
I keep all wastes: transfer pipets, tips, syringes, and beakers, and polymerize them, thinking it makes it all less toxic when it goes to the landfill or waste-to-energy plant.
Tina's Tip: I put my molds and labels in the oven overnight to bake out, and now I never get bubbles!
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
I encountered a strange problem with a Gatan 689 Slow-Scan CCD camera mounted in the 35 mm port above the viewing chamber of a Philips CM100 TEM. When I insert the camera, I can only see a uniform grey image. The histogram shows a single line in the middle. Changing the exposure time or increasing the illumination via condenser 2 has no effect. Allowing the shutter to be normally closed, or putting the shutter to open also has no effect.
There seemed to be no mechanical problem for inserting or retracting the camera because the beam was blocked and blank as it should be.
We are still using DigitalMicrograph 2.5 on an old quadra 840 with system 7.1. I have trashed the preferences and used another copy of the software with no improvement. When the camera is out, I can see the raw image in unprocessed view; basically the dark reference image. With high tension off, I used to see the same image when I insert the camera. But now I only see a grey image. I don't think there is anything wrong with the CCD or scintillator because I can see an after-image of the image I should be seeing when I retract the camera. This suggested that the CCD was exposed and formed an image but somehow not transferred to the computer.
Any suggestion or insight to solve this problem will be much appreciated.
-- Pang (Wai Pang Chan, wpchan-at-u.washington.edu, PAB A087, 206-685-1519) The Biology Imaging Facility (http://staff.washington.edu/wpchan/if/)
I just wanted to add a little information from what I learned in a Statistical Physics course about adiabatic demagnetization 32 years ago as an undergraduate. I worked in a low temperature Physics lab for a summer and I just thought that the application of this was just so cool. (Please, don't pardon the pun. It was intended.) Anyway, the original question asked how it was maintained so I thought that I would add this little description.
The adiabatic demagnetization process uses a paramagnetic salt. When the high magnetic field is turned on, the magnetic moments of the salt aligns with the field. In the Statistical Physics course there was a description of the population of aligned moments over a "kT" term in an exponetional term. The salt is thermally insulated well both mechanically and radiation-wise by the liquid He cooled radiation shield. When the magnetic field is suddenly switched, the population of moments is inverted. The statistical system requires energy to switch the moments and align them with the new magnetic field direction. Since the system is insulated, the only place to take the heat from is from the phonon vibrations of the material itself. The switching of the population into the aligned condition cools the sample by taking the heat from the salt itself. You keep switching the field and it will asymptotically reach a very low equilibrium temperature in the milli-Kelvin range.
Cool, Isn't it. I know, I know. I'm a nerd.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
==============================Original Headers============================== 7, 21 -- From walck-at-southbaytech.com Tue Jul 17 22:54:37 2007 7, 21 -- Received: from flpi101.prodigy.net (flpi101.sbcis.sbc.com [207.115.20.70]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6I3saI7023748 7, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Jul 2007 22:54:37 -0500 7, 21 -- X-ORBL: [64.169.217.123] 7, 21 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) 7, 21 -- by flpi101.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id l6I3sMVA027837 7, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Jul 2007 20:54:22 -0700 7, 21 -- From: "Scott Walck" {walck-at-southbaytech.com} 7, 21 -- To: {Microscopy-at-microscopy.com} 7, 21 -- Subject: RE: [Microscopy] Re: Microcalorimetric EDS 7, 21 -- Date: Tue, 17 Jul 2007 20:54:37 -0700 7, 21 -- Message-ID: {007701c7c8ef$5c766370$7801a8c0-at-dynamicbl8uno3} 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: text/plain; 7, 21 -- charset="us-ascii" 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- X-Mailer: Microsoft Office Outlook 11 7, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 7, 21 -- In-Reply-To: {200707171429.l6HEThLD004349-at-ns.microscopy.com} 7, 21 -- Thread-Index: AcfIfuqOENnW/WTYR+CGa3YPKBPn9gAbDoag ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kenner.rita-at-marshfieldclinic.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] The CAP checklist & ultramicrotomes
Question: Greetings! Does anyone out there have a Leica UC6 ultramicrotome? My lab recently bought one (I love it!), but now I have to rewrite my procedure in accordance with the CAP checklist question ANP.53000. My old ultramicrotome required a lengthy maintenance procedure of cleaning and lubricating parts etc etc. The UC6 is virtually maintenance-free (keep the dust and resin flakes wiped off, and clean the oculars & touch screen as needed). How do you word this in your CAP procedure manual? Thanks for enlightening me - Rita Kenner Electron Microscopist Marshfield Clinic Marshfield, WI 54449
Please forgive me for disturbing your feeling of uniqueness ;-) but we also freeze large batch of resin the same way or almost. The only difference in that we aliquot the resin in syringes, which are already calibrated. The syringes are then closed with parafilm and frozen at -20°C. Syringes are useful because you can use force and you make not mistakes with the volumes (unlike pipetting) and also there will be no remainings sticking to the sides. I must say that I don't even care about warming them up in oven, I just take them out of the freezer and the resin is fluid after 30 min R.T (just the time to concentrate on your experiment while you prepare a cup of coffee). Hope it is useful. Best regards,
Stephane
--- PWebster-at-hei.org wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } } It seems that in many ways my lab may be unique in } its way of handling } resin. Many years ago I threw out the concept of } solutions A and B , and the } WPE values and went instead to a method first } proposed to me by Sus Ito at } Harvard. } } The method has worked consistently on three } different continents and on a } few EM course in different parts of the world. It } consists of mixing three } resin ingredients (but not the catalyst) in a large } volume, aliquoting the } mixture into small glass, screw-top vials and } storing them, tightly closed, } at -20 degrees for as long as it takes to finish the } batch. } } As resin is needed, a vial containing from 4 to 6 ml } of resin is removed } from the freezer and warmed in the oven. The actual } amount of resin in the } vial can be determined by comparing the resin level } with a calibrated (and } marked) black vial). It is important to know the } amount of resin in the } vail because this will determine the amount of } catalyst to use. } } Difficult to embed specimens can be soaked in the } resin without catalyst for } many days, or the resin can be mixed with catalyst } and used immediately. The } advantages are: } } 1. no messy resin preparation needed for each } experiment. } 2. no wasted resin components. } 3. less handling of toxic components. } 4. idiot-proof system ensuring reproducibility. } 5. consistent results - the first vial is the same } as the last vial. } 6. no difficult measuring of catalyst (or any of the } other components). } 7. cleaner than other methods. } } Want to know the method? Here it is: } } Epon Resin (simplified recipe for making large } batches of premixed resin) } } Mix the following in a large glass jar (see below): } } NMA 110 mL } DDSA 130 mL } Eponate 12 230 mL } } When the ingredients have been well mixed, the resin } is poured into small } tubes in aliquots of 4 to 6 mL and stored frozen. } For use, warm a tube and, } using a glass pasteur pipette, add four drops of } BDMA per milliliter (or 2 } drops of DMP-30 if preferred). Mix well, use } immediately and discard unused } resin (polymerization is a good idea). } } Preparing and storing resin this way will ensure } that resin ingredients are } not wasted and all the tubes will have resin of the } same consistency. The } mixture can be tested before use by polymerizing one } tube of resin. All } subsequent tubes of resin will have the same } polymerization and sectioning } qualities as this first tube. } } Glass jar tip: A simple tip for measuring the resin } components is to } pre-calibrate a glass jar. In a clean jar, pour in } 110mL of water, mark the } meniscus. Add an extra 13mL of water and mark the } new meniscus, and finally, } add 230mL of water and mark the meniscus. (For those } in the USA, Smuckers } jars work well - UK, Robinson's orange marmalade - } it just has to be clear } glass!) } } Pour out all the water and dry the jar well. It is } now ready to be used for } measuring out the resin components and mixing them. } Mix the three components } well before pouring the resin into the small vials. } As the volume is } estimated just prior to the resin being used, there } is no need to get into } complicated pipetting methods, just pour carefully. } } As for glass pipettes (and I don't mean to be } confrontational with the } following comments), I have been using them for many } years in my specimen } preparation protocols. In addition to distributing } catalyst, they are very } useful for collecting scraped cells from culture } dishes, and for } transferring solutions from around very small } specimens. } } I am aware that glass fragments are supposed to } enter embedded specimen } blocks and damage diamond knives, but most people I } know also trim their } specimen blocks using glass knives, which is a more } reasonable source of } glass contamination. } } In all the years I have been using glass pipettes I } have never come across } glass fragments in embedded tissues or cell pellets. } I have seen hairs, dust } and large chinks of keratin, but no glass. If glass } were present in the } blocks, I am sure it would be obvious in the initial } trimming and facing } off, which, incidentally we do with an old diamond } knife. } } I send this method out for all to try. If anyone is } brave enough to try it, } let me know if it was useful - I promise that it } will change your life, } especially is you are part of a multi-user facility. } } Best regards, } } Paul Webster. } } } Paul Webster, Ph.D } House Ear Institute } 2100 West Third Street } Los Angeles, CA 90057 } (213) 273 8026 } pwebster-at-hei.org } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I had to giggle at the many replies to my cry of } frustration about } measuring catalyst for resin! Like Tamara, over the } years I have become } adept at sequentially pouring the resin components } into a tri-cornered } beaker, stirring magnetically, then adding catalyst. } Many years ago I used } to measure out the components with syringes and then } laboriously clean the } syringes and keep them for the next use. Once I } switched to weighing I } kept using a tuberculin syringe to measure catalyst } because my crappy } balance couldn't weigh small amounts and did not do } well in the fume } hood; the draft shield was stolen years ago. Then I } got mentally } stuck. My syringes are desperately old, and the } black rubber seal has } been melting when in contact with the catalyst. } } Now when I train people they look at me like I'm } crazy when I pour, so I } let them transfer components to the beaker on the } balance with disposable } === message truncated ===
____________________________________________________________________________________ TV dinner still cooling? Check out "Tonight's Picks" on Yahoo! TV. http://tv.yahoo.com/
We weigh out all our resin components. And use a disposable pipet to weigh out dropwise.
When working with a new resin mix for the first time if it does not have weights (only volumes) we carefully weighout each volumeterically measured component and right it down.
On 16 Jul 2007 at 19:43, tina-at-pbrc.hawaii.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, all- } } Here's kind of a dumb question. How do you all measure out embedding resin } catalyst? I've only been doing it for 31 years... I used to use tuberculin } syringes - had to hurry because they sort of melt. The current crop of } facility users seem dumbfounded that I would use anything but a } pipetter. But I have now had another new pipetter get gummed up with some } resin component, probably catalyst. Does anyone have a favorite method??? } } Aloha with exasperation, } Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } **************************************************************************** } } } ==============================Original Headers============================== } 5, 19 -- From tina-at-pbrc.hawaii.edu Mon Jul 16 19:41:40 2007 } 5, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6H0fcsx005560 } 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 16 Jul 2007 19:41:39 -0500 } 5, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) } 5, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id l6H0fZfB008820 } 5, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) } 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 16 Jul 2007 14:41:36 -1000 (HST) } 5, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id l6H0fY0E008817 } 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 16 Jul 2007 14:41:35 -1000 (HST) } 5, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs } 5, 19 -- Date: Mon, 16 Jul 2007 14:41:33 -1000 (HST) } 5, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} } 5, 19 -- X-Sender: tina-at-halia } 5, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} } 5, 19 -- Subject: pipeting resin components } 5, 19 -- Message-ID: {Pine.GSO.4.21.0707161437130.8234-100000-at-halia} } 5, 19 -- MIME-Version: 1.0 } 5, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 8, 25 -- From edelmare-at-muohio.edu Wed Jul 18 08:24:21 2007 8, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6IDOKTG007308 8, 25 -- for {microscopy-at-Microscopy.com} ; Wed, 18 Jul 2007 08:24:21 -0500 8, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 8, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l6IDOK3g020010; 8, 25 -- Wed, 18 Jul 2007 09:24:20 -0400 8, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 8, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l6IDOJvh005623; 8, 25 -- Wed, 18 Jul 2007 09:24:20 -0400 8, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 8, 25 -- To: tina-at-pbrc.hawaii.edu 8, 25 -- Date: Wed, 18 Jul 2007 09:24:19 -0400 8, 25 -- MIME-Version: 1.0 8, 25 -- Subject: Re: [Microscopy] pipeting resin components 8, 25 -- CC: microscopy-at-Microscopy.com 8, 25 -- Message-ID: {469DDC43.16716.18219BE5-at-edelmare.muohio.edu} 8, 25 -- Priority: normal 8, 25 -- In-reply-to: {200707170043.l6H0h0I6006770-at-ns.microscopy.com} 8, 25 -- References: {200707170043.l6H0h0I6006770-at-ns.microscopy.com} 8, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 8, 25 -- Content-type: text/plain; charset=US-ASCII 8, 25 -- Content-transfer-encoding: 7BIT 8, 25 -- Content-description: Mail message body 8, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
You make no mention of when you add cataalyst to the resin. Do youincorporate ti into the final mixture that you freeze, or do you add it just before you use the resin?
There is a bg difference. If you add it to the resin before freezing, the resin will slowly harder, even at -20 degrees. This means that the properties of the resin are changing during storage. It also means the resin mixture can't be stored for long periods. We are able to store our uncatalysed resin for more than a year.
A possible complication of storing resin in plastic is that some plastics contain unincorporated plasticizer that could potentially inhibit, or otherwise alter the polymerization of the resin. Usually this is only a problem when setting up a new lab where the consumables are not exactly the same.
One usinque example of plastiziser interfering with polymerization is when polymerization of Lowicryl is attempted in new Eppendorf tubes. The new tubes have a volatile substace present that completely inhibits polymerization. The solution is to either autoclave the tubes, heat them in an oven overnight, or leave them in a cupboard for a few years.
My apologies for the errors in the last post, my finers refuse to learn how to spell.
Regards,
Paul.
House Ear Institute 2100 West Third Street Los Angeles, CA 90057.
-----Original Message----- X-from: Stephane Nizet [mailto:nizets2-at-yahoo.com] Sent: Wed 7/18/2007 1:14 AM To: Webster, Paul Cc: microscopy-at-microscopy.com
In my earlier reply to Tina, I addressed only her immediate question: how to measure out the catalyst. In response to Paul's posting, I do a very similar thing, except that I use a method taught to me by my first boss, the late Tom Robinson: I store the resin in the freezer, in syringes. I cap the end (you can buy syringe caps from the suppliers), and wrap the tips with parafilm. Works like a charm. If you use Spurr's resin, you can even store it with the catalyst mixed in for a few weeks at -20. The syringes, obviously, make measuring the volume quite easy. Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Weill Cornell Medical College
My lab has been using a system similar to Dr. Webster's for 30 years. The "Epon" resin components, minus the accelerator, are made up in batches of 200-400ml, thoroughly mixed and placed in glass vials in commonly used aliquots (5,10,15,20,30,40ml). Some vials are left half filled to allow for 1:1 resin mixes. They are tightly sealed and stored at -20 degrees. Before use the vials are allowed to come to room temperature and DMP is added. The DMP is measured in disposable 1mm BD tuberculin syringes, without needles. At about a nickel each, why wash glass syringes? As pointed out by others, the resin can be stored much longer without the accelerator. And even before resin stored with the accelerator added becomes unusable, it will be partially polymerized and more viscous than it should be. We also use disposable glass pipettes without problems.
Ralph Common Michigan State University
==============================Original Headers============================== 4, 24 -- From rcommon-at-msu.edu Wed Jul 18 09:42:28 2007 4, 24 -- Received: from sys27.mail.msu.edu (sys27.mail.msu.edu [35.9.75.127]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6IEgSWw012898 4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jul 2007 09:42:28 -0500 4, 24 -- Received: from [35.9.122.125] (helo=emlab) 4, 24 -- by sys27.mail.msu.edu with esmtpsa (Exim 4.63 #1) 4, 24 -- (TLSv1:RC4-MD5:128) 4, 24 -- id 1IBAjT-0006a1-72 4, 24 -- for Microscopy-at-microscopy.com; Wed, 18 Jul 2007 10:42:27 -0400 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: pipeting resin components 4, 24 -- Date: Wed, 18 Jul 2007 10:43:55 -0400 4, 24 -- Message-ID: {000501c7c94a$12a53bd0$7d7a0923-at-msu.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="iso-8859-1" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 (Normal) 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1896 4, 24 -- Importance: Normal 4, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
I am following this discussion with great interest, since I've heard before about storing resins sans accelerator and adding this during the final infiltration steps. We also store resins in the freezer, but we use so much that it usually never has the chance to polymerize significantly before it is used, so we store it with the accelerator pre-mixed but there are times when it would be very convenient to make huge resin batches or smaller batches of infrequently used resins, and know that a month later they would still be predictably usable.
My questions are: regarding resins with and without the accelerator, what are your infiltration schedules, and if the resin/accelerator mix is added at the last step, do you always get consistent infiltration of the accelerator into the tissue? In other words, isn't is possible to get inconsistently hardened blocks if the the accelerator is added during the last one or two changes of pure resin and doesn't get completely and uniformly distributed in the tissue?
Thanks much, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 5, 23 -- From TindallR-at-missouri.edu Wed Jul 18 09:55:59 2007 5, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6IEtxIk024986 5, 23 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jul 2007 09:55:59 -0500 5, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 5, 23 -- Wed, 18 Jul 2007 09:55:58 -0500 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 23 -- Content-class: urn:content-classes:message 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="us-ascii" 5, 23 -- Subject: Pipetting resins: Question 5, 23 -- Date: Wed, 18 Jul 2007 09:55:59 -0500 5, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BBD4-at-UM-XMAIL08.um.umsystem.edu} 5, 23 -- X-MS-Has-Attach: 5, 23 -- X-MS-TNEF-Correlator: 5, 23 -- Thread-Topic: Pipetting resins: Question 5, 23 -- Thread-Index: AcfJS8KjxuxCLd/tQe60B82iCyTRLg== 5, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- X-OriginalArrivalTime: 18 Jul 2007 14:55:58.0856 (UTC) FILETIME=[C040F080:01C7C94B] 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l6IEtxIk024986 ==============================End of - Headers==============================
Dear All, I just bought a used Beckman Airfuge which came with this rotor:
www.elektronenmikroskopie.info/airfuge_rotor/
Can anybody tell me which rotor this is? For what purposes it will normally be used? What vials / inlays are needed? The Airfuge spins up nicely with this rotor...
I would like to centrifuge virus particles on TEM grids. I understand, that I may need another rotor. Which one would be best? Is a used one available out there for "not so much" money?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Ian.Lamswood-at-leica-microsystems.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Ian.Lamswood-at-leica-microsystems.com Name: Ian Lamswood
Organization: Leica Microsystems
Title-Subject: [Filtered] Job Opportunity
Question: Leica Microsystems, Vienna has an exciting opportunity in our Business Unit for a Product Manager. Joining the team, you would be responsible for the complete life cycles of the cryo-instruments in our EM sample preparation product line. These include high pressure freezer and freeze substitution instruments. The position is based in Vienna with international travel.
Experience We are looking for someone with a biological background and commercial training, with a minimum of 3 years work experience in the areas of sales, marketing and/or product management. Knowledge of sample preparation equipment would be an advantage. A readiness to travel is also required along with a good understanding of MS Office. Fluent spoken and written English is essential.
If you are interested in this position please send your CV by e-mail to Otmar.Kases-at-leica-microsystems.com
Hi all I am doing immuno-EM on human Red Blood Cells. In the past I have used cryo-sections and probing on the grid. I would like to plastic embed the cells and do antibody labeling on the grid. I used my normal protocol and embedded in unicryl. Then my problem occurred. The unicryl block polymerizes (UV for a few days at -10°C) just fine except in the sample pellet. I assume the UV light is being absorbed by the RBCs, and thus not penetrating the sample.
So, my question is, what should I try next?
Thank you David
_____________________
David Elliott Ph.D. Assistant Professor - Department of Cell Biology and Anatomy Director, Research Microscopy Core Service University of Arizona College of Medicine PO Box 245004 Tucson, AZ 85724
Voice: 520-626-7870 Fax: 520-626-2097
==============================Original Headers============================== 9, 21 -- From Elliott-at-arizona.edu Wed Jul 18 20:35:06 2007 9, 21 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.132.44]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6J1Z6YL011073 9, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 18 Jul 2007 20:35:06 -0500 9, 21 -- Received: from gandalfs_amavis (amavis5.email.arizona.edu [10.0.0.208]) 9, 21 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 4EF3278C16 9, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 18 Jul 2007 18:35:06 -0700 (MST) 9, 21 -- Received: from [192.168.0.30] (doctorelliott.us [70.57.226.104]) 9, 21 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 21407788EC 9, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 18 Jul 2007 18:35:04 -0700 (MST) 9, 21 -- Mime-Version: 1.0 (Apple Message framework v752.2) 9, 21 -- Message-Id: {AAB76F18-1910-49CC-BC00-D10CEF6C5D45-at-arizona.edu} 9, 21 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 9, 21 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 9, 21 -- From: David Elliott {Elliott-at-arizona.edu} 9, 21 -- Subject: immuno-EM of Red Blood Cells 9, 21 -- Date: Wed, 18 Jul 2007 18:35:01 -0700 9, 21 -- X-Mailer: Apple Mail (2.752.2) 9, 21 -- X-Virus-Scanned: amavisd-new at email.arizona.edu 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l6J1Z6YL011073 ==============================End of - Headers==============================
Hi, Why is the vacuum gauge positioned at a right angle to the vertical tube to the vacuum system on JEOL 733's and 8600's (and others in this line)?
Mike
******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html ********************************************************************
==============================Original Headers============================== 6, 18 -- From mmcheath-at-syr.edu Thu Jul 19 08:54:04 2007 6, 18 -- Received: from SUEXCL-02.ad.syr.edu (suexbe-02.ad.syr.edu [128.230.108.46]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6JDs34H015493 6, 18 -- for {microscopy-at-microscopy.com} ; Thu, 19 Jul 2007 08:54:03 -0500 6, 18 -- Received: from 128.230.24.156 ([128.230.24.156]) by SUEXCL-02.ad.syr.edu ([128.230.108.53]) via Exchange Front-End Server exchange.syr.edu ([128.230.108.49]) with Microsoft Exchange Server HTTP-DAV ; 6, 18 -- Thu, 19 Jul 2007 13:54:01 +0000 6, 18 -- User-Agent: Microsoft-Entourage/11.3.3.061214 6, 18 -- Date: Thu, 19 Jul 2007 09:53:59 -0400 6, 18 -- Subject: JEOL 733's and 8600's: position of vacuum gauge 6, 18 -- From: Michael Cheatham {mmcheath-at-syr.edu} 6, 18 -- To: {microscopy-at-microscopy.com} 6, 18 -- Message-ID: {C2C4E537.162EB%mmcheath-at-syr.edu} 6, 18 -- Thread-Topic: JEOL 733's and 8600's: position of vacuum gauge 6, 18 -- Thread-Index: AcfKDEF1gDay2DX/EdyL+AAWy6AryQ== 6, 18 -- Mime-version: 1.0 6, 18 -- Content-type: text/plain; 6, 18 -- charset="US-ASCII" 6, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
If the UV is the problem, you will solve it by using LR White resin with the accelerator. It will easily polymerizes at 4 degree using 1.5 microliter of accelerator for 1 ml of resin.
} David, } } First question: you don't mention osmium -- I suspect your samples } are not osmicated, but just to be sure: are they?
They are not.
} Osmium interfers with polymerization of Unicryl and other acrylates.
It also interferes with antigenisity.
} If you're not, then I think you're right, the pellet is blocking } the UV. I'd try dispersing the RBCs in agar, so the pellet isn't } dense with cells.
The cells were dispersed in gelatin, but I could try making the cells less concentrated.
} Otherwise: are you looking for a plasma membrane bound epitope? If } yes, you should get good results with Au conjugated antibody } labelling and FE-SEM, cryo or chemically fixed. Lot more to be seen } this way, including patterns of distribution of the epitope on the } membrane, something that's very difficult to find with sections.
No, I am looking at internal epitopes.
Thanx for the suggestions.
David } } Phil } } } --------------------------------------------------------------------- } } ------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------- } } ------- } } } } Hi all } } I am doing immuno-EM on human Red Blood Cells. In the past I have } } used cryo-sections and probing on the grid. I would like to plastic } } embed the cells and do antibody labeling on the grid. I used my } } normal protocol and embedded in unicryl. Then my problem occurred. } } The unicryl block polymerizes (UV for a few days at -10ƒC) just fine } } except in the sample pellet. I assume the UV light is being absorbed } } by the RBCs, and thus not penetrating the sample. } } } } So, my question is, what should I try next? } } } } Thank you } } David } } } } } } } } } } _____________________ } } } } David Elliott Ph.D. } } Assistant Professor - Department of Cell Biology and Anatomy } } Director, Research Microscopy Core Service } } University of Arizona College of Medicine } } PO Box 245004 } } Tucson, AZ 85724 } } } } Voice: 520-626-7870 } } Fax: 520-626-2097 } -- } Philip Oshel } Microscopy Facility Supervisor } Biology Department } 024C Brooks Hall } Central Michigan University } Mt. Pleasant, MI 48859
==============================Original Headers============================== 14, 23 -- From Elliott-at-arizona.edu Thu Jul 19 11:37:49 2007 14, 23 -- Received: from smtpgate.email.arizona.edu (frodo.email.arizona.edu [128.196.132.210]) 14, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6JGbmtE010592 14, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 19 Jul 2007 11:37:49 -0500 14, 23 -- Received: from frodos_amavis (amavis5.email.arizona.edu [10.0.0.208]) 14, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 4872B5625A 14, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 19 Jul 2007 09:37:42 -0700 (MST) 14, 23 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 14, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 7A9A25A401 14, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 19 Jul 2007 09:37:41 -0700 (MST) 14, 23 -- Mime-Version: 1.0 (Apple Message framework v752.2) 14, 23 -- In-Reply-To: {f06240800c2c507b38cf4-at-[141.209.160.249]} 14, 23 -- References: {200707190139.l6J1dgx4017791-at-ns.microscopy.com} {f06240800c2c507b38cf4-at-[141.209.160.249]} 14, 23 -- Content-Type: text/plain; charset=WINDOWS-1252; delsp=yes; format=flowed 14, 23 -- Message-Id: {4888E063-07D6-493F-9BAB-5B16205D7481-at-arizona.edu} 14, 23 -- From: David Elliott {Elliott-at-arizona.edu} 14, 23 -- Subject: Re: [Microscopy] immuno-EM of Red Blood Cells 14, 23 -- Date: Thu, 19 Jul 2007 09:37:45 -0700 14, 23 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 14, 23 -- X-Mailer: Apple Mail (2.752.2) 14, 23 -- X-Virus-Scanned: amavisd-new at email.arizona.edu 14, 23 -- Content-Transfer-Encoding: 8bit 14, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l6JGbmtE010592 ==============================End of - Headers==============================
We are running up against a deadline trying to image bacteria in root nodules of a legume. Everything is working fine, except for the small detail of myriads of holes in the resin wherever there are clusters of bacteria.
First attempt was with Spurr's resin, using extended microwaving in the processing and infiltration. By extended, I mean microwaving each sample twice under vacuum at each infiltration step, followed by time on a rocker (several hours to overnight) before going on to the next infiltration step. This was a multi-day procedure. Holes, holes, holes.
We next tried using LR White and an even more extended procedure (more infiltration steps, more changes of pure resin) that has proven successful at embedding whole retinas. Holes.......
The 100nm sections are on carbon-coated grids 200 mesh Cu grids. Ultrastructure of the sample is fine and there are no holes anywhere except where the bacteria are clustered, which, of course, is where we need the pictures.
Any ideas? Anyone?
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 10, 23 -- From TindallR-at-missouri.edu Thu Jul 19 12:03:19 2007 10, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6JH3JW6022822 10, 23 -- for {microscopy-at-microscopy.com} ; Thu, 19 Jul 2007 12:03:19 -0500 10, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 10, 23 -- Thu, 19 Jul 2007 12:03:18 -0500 10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 23 -- Content-class: urn:content-classes:message 10, 23 -- MIME-Version: 1.0 10, 23 -- Content-Type: text/plain; 10, 23 -- charset="us-ascii" 10, 23 -- Subject: TEM: Bacteria in root nodules 10, 23 -- Date: Thu, 19 Jul 2007 12:03:19 -0500 10, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BBE8-at-UM-XMAIL08.um.umsystem.edu} 10, 23 -- X-MS-Has-Attach: 10, 23 -- X-MS-TNEF-Correlator: 10, 23 -- Thread-Topic: TEM: Bacteria in root nodules 10, 23 -- Thread-Index: AcfKJrgC4M87uwT3SECEvZirIqfFpQ== 10, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 10, 23 -- To: {microscopy-at-microscopy.com} 10, 23 -- X-OriginalArrivalTime: 19 Jul 2007 17:03:18.0821 (UTC) FILETIME=[B470D550:01C7CA26] 10, 23 -- Content-Transfer-Encoding: 8bit 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l6JH3JW6022822 ==============================End of - Headers==============================
I had had problems with Listeria in monocytes back in the '80s. After much grief of having holes inside the bacteria as well as peppering that formed as the beam hit the bacterium (sorry to say published figures - the paper was finished and went to press), I discovered that the cause was not only in the infiltration, which I had already extended, but also in the dehydration.
The 'cure' was to increase time in all steps and add an additional exchange of 100% EtOH (3 changes) for the bacteria were much more dense than the surrounding tissue and tended to hold on to the moisture.
I was not using microwave so I do not know how much additional time would be required for that technique.
I'm interested to see suggestions from others too. Pat
Patricia Stranen Connelly NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 connellyps-at-mail.nih.gov
On 7/19/07 1:09 PM, "TindallR-at-missouri.edu" {TindallR-at-missouri.edu} wrote:
} Dear Listers, } } Holey Rhizobia, Batman! } } We are running up against a deadline trying to image bacteria in root } nodules of a legume. Everything is working fine, except for the small } detail of myriads of holes in the resin wherever there are clusters of } bacteria. } } First attempt was with Spurr's resin, using extended microwaving in the } processing and infiltration. By extended, I mean microwaving each } sample twice under vacuum at each infiltration step, followed by time on } a rocker (several hours to overnight) before going on to the next } infiltration step. This was a multi-day procedure. Holes, holes, } holes. } } We next tried using LR White and an even more extended procedure (more } infiltration steps, more changes of pure resin) that has proven } successful at embedding whole retinas. Holes....... } } The 100nm sections are on carbon-coated grids 200 mesh Cu grids. } Ultrastructure of the sample is fine and there are no holes anywhere } except where the bacteria are clustered, which, of course, is where we } need the pictures. } } Any ideas? Anyone? } } Cheers, } Randy } } Randy Tindall } Senior EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 24 -- From connellyps-at-nhlbi.nih.gov Thu Jul 19 13:09:17 2007 10, 24 -- Received: from NIHCESSMTP2.hub.nih.gov (nihcessmtp2.hub.nih.gov [128.231.90.116]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6JI9GrK003803 10, 24 -- for {microscopy-at-microscopy.com} ; Thu, 19 Jul 2007 13:09:17 -0500 10, 24 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 10, 24 -- Thu, 19 Jul 2007 14:09:11 -0400 10, 24 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.168]) with Microsoft Exchange Server HTTP-DAV ; 10, 24 -- Thu, 19 Jul 2007 18:09:11 +0000 10, 24 -- User-Agent: Microsoft-Entourage/11.3.3.061214 10, 24 -- Date: Thu, 19 Jul 2007 14:08:22 -0400 10, 24 -- Subject: Re: [Microscopy] TEM: Bacteria in root nodules 10, 24 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 10, 24 -- To: Randy Tindall {TindallR-at-missouri.edu} 10, 24 -- CC: {microscopy-at-microscopy.com} 10, 24 -- Message-ID: {C2C520D6.8F1%connellyps-at-nhlbi.nih.gov} 10, 24 -- Thread-Topic: [Microscopy] TEM: Bacteria in root nodules 10, 24 -- Thread-Index: AcfKL8rqCayRuDYjEdy+ygANk2Yv1A== 10, 24 -- In-Reply-To: {200707191709.l6JH938e031492-at-ns.microscopy.com} 10, 24 -- Mime-version: 1.0 10, 24 -- Content-type: text/plain; 10, 24 -- charset="ISO-8859-1" 10, 24 -- X-OriginalArrivalTime: 19 Jul 2007 18:09:11.0944 (UTC) FILETIME=[E8AF8880:01C7CA2F] 10, 24 -- Content-Transfer-Encoding: 8bit 10, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l6JI9GrK003803 ==============================End of - Headers==============================
Fortunately you solution is easy - cut the gelatin embedded blocks of red blood cells into very thin slices and try your embedding again. The thinner slices will give more access tot he UV light and you will get the resin polymerized.
I have embedded gelatin-immobilized malaria-infected red blood cells in Lowicryl (polymerizing with UV light) on many occasions so I know the trick works.
Now, just don't ask me how to get rid of all the non-specific labeling over the haemoglobin!
Regards,
Paul.
House Ear Institute 2100 W 3rd St Los Angeles CA 90057
-----Original Message----- X-from: Elliott-at-arizona.edu [mailto:Elliott-at-arizona.edu] Sent: Wed 7/18/2007 6:39 PM To: Webster, Paul
Hi all I am doing immuno-EM on human Red Blood Cells. In the past I have used cryo-sections and probing on the grid. I would like to plastic embed the cells and do antibody labeling on the grid. I used my normal protocol and embedded in unicryl. Then my problem occurred. The unicryl block polymerizes (UV for a few days at -10°C) just fine except in the sample pellet. I assume the UV light is being absorbed by the RBCs, and thus not penetrating the sample.
So, my question is, what should I try next?
Thank you David
_____________________
David Elliott Ph.D. Assistant Professor - Department of Cell Biology and Anatomy Director, Research Microscopy Core Service University of Arizona College of Medicine PO Box 245004 Tucson, AZ 85724
Voice: 520-626-7870 Fax: 520-626-2097
==============================Original Headers============================== 9, 21 -- From Elliott-at-arizona.edu Wed Jul 18 20:35:06 2007 9, 21 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.132.44]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6J1Z6YL011073 9, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 18 Jul 2007 20:35:06 -0500 9, 21 -- Received: from gandalfs_amavis (amavis5.email.arizona.edu [10.0.0.208]) 9, 21 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 4EF3278C16 9, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 18 Jul 2007 18:35:06 -0700 (MST) 9, 21 -- Received: from [192.168.0.30] (doctorelliott.us [70.57.226.104]) 9, 21 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 21407788EC 9, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 18 Jul 2007 18:35:04 -0700 (MST) 9, 21 -- Mime-Version: 1.0 (Apple Message framework v752.2) 9, 21 -- Message-Id: {AAB76F18-1910-49CC-BC00-D10CEF6C5D45-at-arizona.edu} 9, 21 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 9, 21 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 9, 21 -- From: David Elliott {Elliott-at-arizona.edu} 9, 21 -- Subject: immuno-EM of Red Blood Cells 9, 21 -- Date: Wed, 18 Jul 2007 18:35:01 -0700 9, 21 -- X-Mailer: Apple Mail (2.752.2) 9, 21 -- X-Virus-Scanned: amavisd-new at email.arizona.edu 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l6J1Z6YL011073 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 21 -- From PWebster-at-hei.org Thu Jul 19 13:24:46 2007 23, 21 -- Received: from hi0sml1.hei.org (House-Ear-Institute-1114861.cust-rtr.pacbell.net [71.136.31.242] (may be forged)) 23, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6JIOkEI015748 23, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 19 Jul 2007 13:24:46 -0500 23, 21 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 23, 21 -- Content-class: urn:content-classes:message 23, 21 -- MIME-Version: 1.0 23, 21 -- Content-Type: text/plain; 23, 21 -- charset="iso-8859-1" 23, 21 -- Subject: RE: [Microscopy] immuno-EM of Red Blood Cells 23, 21 -- Date: Thu, 19 Jul 2007 11:20:51 -0700 23, 21 -- Message-ID: {87449E4A2B01DA47B29424CE5D6E0F8304178FBA-at-hi0sml1.hei.org} 23, 21 -- X-MS-Has-Attach: 23, 21 -- X-MS-TNEF-Correlator: 23, 21 -- Thread-Topic: [Microscopy] immuno-EM of Red Blood Cells 23, 21 -- Thread-Index: AcfJpagE7M9DZrOnSIqOcG4ydBiLhgAi+Hqd 23, 21 -- References: {200707190139.l6J1dWIk017519-at-ns.microscopy.com} 23, 21 -- From: "Webster, Paul" {PWebster-at-hei.org} 23, 21 -- To: {Elliott-at-arizona.edu} , {Microscopy-at-MSA.Microscopy.Com} 23, 21 -- Content-Transfer-Encoding: 8bit 23, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l6JIOkEI015748 ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (brachfelds-at-mail.montclair.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, July 19, 2007 at 14:33:07 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both brachfelds-at-mail.montclair.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: I am a faculty member purchasing a polarizing microscope for transmitted and reflected light analysis of geologic samples. I am new to light microscopy, and I am curious if anyone has experience and recommendations (performance, maintenance, frequency of repairs, quirks, etc.) on the different models of scopes that I am considering. They include the Leica DM2500, Zeiss Axioscope, Nikon LV100, and Olympus CX. Thank you.
Dear Foo Li, You asked for confirmation of the stated 30 nm resolution of the FEI Phenom table top SEM.
1) The general advice when buying any scientific instrument is that you should confirm that it has the capabilities that you require for your work. In this case, you should not rely on a nominal resolution figure that may relate to materials very different from yours. So, I recommend you contact the FEI Phenom group to arrange a demo on your materials.
2) I saw a demo of this unit at the Semicon West expo this week. The test specimen is a 144-nm pitch, 2-dimensional grip of Aluminum bumps on Silicon. The pattern was barely visible, with very poor contrast. In a good FE-SEM, this pattern can easily be imaged. See http://www.asmicro.com/Supplies/150-2D-Calibration-Specimen.htm and scroll down to the SEM images.
3) Although the Phenom SEM could not make a useful image of the high resolution pattern, I was impressed with the ease of use. They have cleverly combined video OM imaging with a touch-screen display to provide fingertip navigation on the specimen. Sample navigation is much more natural here than in the Hitachi TM-1000, which made slightly better images of the same pattern.
4) Disclaimer: I am not an expert on current SEM instrumentation. I'm an AFM expert who uses SEM from time to time.You need to form your own opinion. regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] ============================================= ----- Original Message ----- From: fli-at-estee.com To: donc-at-asmicro.com Sent: Tuesday, July 17, 2007 9:32 AM Subject: [a] [Microscopy] AskAMicroscopist: FEI Phenom Electron-light microscope does it
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html ----------------------------------------------------------------------------
This Question was submitted to Ask-A-Microscopist by (fli-at-estee.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, July 17, 2007 at 08:08:29 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both fli-at-estee.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: fli-at-estee.com Name: Foo Li
Organization: Estee Lauder
Education: Graduate College
Location: Melville, NY 11747
Title: FEI Phenom Electron-light microscope
Question: Is the Phenom the real deal? 30 nm resolution is not all that bad especially when dealing with emulsions. I need an opinion of this product from actual Phenom users out there.
Does anyone have a general reference or review that is appropriate for describing the technique of cryo-SEM? Here are lots of papers utilizing cryo0SEM but I am looking for one that deals with introducing the technique in addition to the resulting science.
Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 6, 21 -- From dsherman-at-purdue.edu Fri Jul 20 07:31:28 2007 6, 21 -- Received: from 1061exfe04.adpc.purdue.edu (1061exfe04.adpc.purdue.edu [128.210.63.227]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6KCVSvx021783 6, 21 -- for {microscopy-at-microscopy.com} ; Fri, 20 Jul 2007 07:31:28 -0500 6, 21 -- Received: from exchange.purdue.edu ([172.21.6.23]) by 1061exfe04.adpc.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 6, 21 -- Fri, 20 Jul 2007 08:31:28 -0400 6, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 6, 21 -- Fri, 20 Jul 2007 12:31:25 +0000 6, 21 -- User-Agent: Microsoft-Entourage/11.3.6.070618 6, 21 -- Date: Fri, 20 Jul 2007 08:31:25 -0400 6, 21 -- Subject: Reference fo cryo-SEM 6, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 6, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 21 -- Message-ID: {C2C6235D.1F5ED%dsherman-at-purdue.edu} 6, 21 -- Thread-Topic: Reference fo cryo-SEM 6, 21 -- Thread-Index: AcfKyeMPIWa7iDa9EdyWtwARJN08Mg== 6, 21 -- Mime-version: 1.0 6, 21 -- Content-type: text/plain; 6, 21 -- charset="US-ASCII" 6, 21 -- Content-transfer-encoding: 7bit 6, 21 -- X-OriginalArrivalTime: 20 Jul 2007 12:31:28.0462 (UTC) FILETIME=[E51F4EE0:01C7CAC9] ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fab-at-tariffenet.it as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: Dear All, I made an immunocytochemical reaction on paraffin section with HRP and DAB. Negative controls (just only without primary ab)are OK, but reaction slides show a diffuse and homoeneous cytoplasmic staining: What is the meaning of such results?
I just saw something called "Quick Boats" on the EMS website. This is an aluminum boat that clamps on to a glass knife with a set screw and silicon rubber gasket. it is meant to be a reusable version of a Truf or metal tape boat. Has anyone used one of these? Do they last with heavy use? Off-line or Or-line responses welcomed. thanks, tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
My first reaction would be to answer: your antigen has a diffuse and homogeneous cytoplasmic pattern!
Now you must consider that HRP and DAB are enzymatic reaction, which means that the product of the reaction diffuses a bit. It is one of the major drawbacks of this detection method. If you want precise intracellular localisations, try immunofluorescence and laser scanning confocal microscopy.
Best regards,
Stephane
--- fab-at-tariffenet.it wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the } Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a } Subscriber, so when replying } please copy both fab-at-tariffenet.it as well as } the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: fab-at-tariffenet.it } Name: Fabio } } Organization: University of Catania } } Title-Subject: [Filtered] Light immunohistochemistry } } Question: Dear All, } I made an immunocytochemical reaction on paraffin } section with HRP and DAB. Negative controls (just } only without primary ab)are OK, but reaction slides } show a diffuse and homoeneous cytoplasmic staining: } What is the meaning of such results? } } Thank You } } fabio } UniCT } } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Fri Jul 20 } 08:22:29 2007 } 8, 11 -- Received: from [206.69.208.22] } (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l6KDMRuO002255 } 8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 20 } Jul 2007 08:22:28 -0500 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: } {p06240809c2c66785e6df-at-[206.69.208.22]} } 8, 11 -- Date: Fri, 20 Jul 2007 08:22:27 -0500 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: fab-at-tariffenet.it (by way of } MicroscopyListserver) } 8, 11 -- Subject: rviaWWW: Light } immunohistochemistry } 8, 11 -- Content-Type: text/plain; } charset="us-ascii" } ==============================End of - } Headers============================== }
____________________________________________________________________________________ Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. http://sims.yahoo.com/
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dlowry-at-asu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dlowry-at-asu.edu Name: David Lowry
Organization: Arizona State University
Title-Subject: [Filtered] tannic acid
Question: Does tannic acid negatively affect antigenicity of epitopes for on-section immuno-labeling? I have used it previously during freeze-substitution for standard thin-section EM but am not aware of how it may affect labeling. If anyone may have a protocol or other advice they would be willing to share, it would be greatly appreciated.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both hainfeld-at-bnl.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: hainfeld-at-bnl.gov Name: James F. Hainfeld
Organization: Brookhaven National Lab
Title-Subject: [Filtered] gold labeling workshop
Question: If you previously applied and have not been notified, please contact us again.
Gold Labeling Workshop Sept. 11-13, 2007 Sponsored by the Brookhaven National Lab STEM (scanning transmission EM) Facility A Hands-on Tutorial that will cover both theory and practice. Topics will include: Gold nanoparticles: structure, synthesis, and properties Molecular labeling strategies (to amines, thiols, genetic tags, active sites, complexes, nucleic acids, peptides) Ni-NTA-gold Sizes of gold from 0.8 nm to 30 nm Isolation of labeled products (various chromatographies and other methods) Unstained, negatively stained and Cryo samples Comparison of STEM and TEM Silver and gold enhancement Image processing using gold labels Problems and how to overcome them (e.g., labeling yield/occupancy, non-specific binding) Other uses of gold nanoparticles (Western blots, light microscopy, medicine) Laboratory participation to gold label a protein for EM Whatís new
Limited Enrollment due to lab space: A course fee of $650 covers housing, meals (dorm, cafeteria), and materials. Proposals to attend will be refereed: submit CV and letter describing you and your labís labeling interest. Optionally describe a sample of yours that, if selected, would be labeled during the course. Submit registration request to: Dr. James Hainfeld by email: hainfeld-at-bnl.gov Brookhaven National Laboratory, Biology Dept., Bldg. 463, Upton, NY 11973, USA Tel. 631-344-3372 * Fax
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ech-at-interchange.ubc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: Dear all Two years ago in Hawaii at the M&M meeting, Gracie Burke, then MSA President, initiated the "Family Affair" to make microscopy fun for the families who travelled with delegates of the conference and to give them an opportunity to visit the Exhibition floor. My talk was based around the theme of using microscopy to "Solve a Mystery." Since the next M&M is in Florida, it is expected that several families will be attending and we are extending the "Family Affair" to Monday and Tuesday afternoons.
The general theme of the talks will again be "Solve the Mystery" (different mysteries from Hawaii) using microscopy with a biological emphasis on Monday and a materials emphasis on Tuesday.
Since the convention center is located in the Port of Ft. Lauderdale, an ID must be shown for access to the port. Anyone can enter the port, then the convention center, after showing an ID. This is a reminder to make sure that when you bring the family to the convention centre they should all have IDs with them. This is not a problem for those families arriving by air, but families driving to Ft. Lauderdale may not think to pack IDs for the kids.
I am wondering whether anybody knows a /citation /for the lateral resolution of immunogold stains. I have ± 20nm in my head (this should correspond to the lenth of two antibody molecules if there is one primary and one secondary antibody between the gold granules and the epitope) and don't know whether I read this from a posting a while ago or in an article.
thank you - as always
Gerd
Dr Gerd Leitinger Institute of Cell Biology, JHistology and Embryology Medical University of Graz, Austria
Hello: We are going to a web based sign up for a new characterization facility that will contain about 10 instruments. We have done a search of the archives and found 3 systems, FACES and one from Northwestern and U. of Arizona. Are there others that people are using we should consider. Thanks. Steve
Stephen McCartney Senior Research Associate Nanoscale Characterization and Fabrication Laboratory (NCFL) Institute for Critical Technology and Applied Science (ICTAS) 1991 Kraft Drive Va Tech Blacksburg, VA 24061 540-231-9765
==============================Original Headers============================== 3, 20 -- From stmccart-at-vt.edu Mon Jul 23 08:47:11 2007 3, 20 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu [198.82.162.213]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6NDlBen001511 3, 20 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 08:47:11 -0500 3, 20 -- Received: from vivi.cc.vt.edu (IDENT:mirapoint-at-evil-vivi.cc.vt.edu [10.1.1.12]) 3, 20 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id l6NDlAZL019144 3, 20 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 09:47:10 -0400 3, 20 -- Received: from mri-system.vt.edu ([198.82.148.26]) 3, 20 -- by vivi.cc.vt.edu (MOS 3.8.2-GA) 3, 20 -- with ESMTP id HTD69749; 3, 20 -- Mon, 23 Jul 2007 09:47:10 -0400 (EDT) 3, 20 -- Message-Id: {5.2.1.1.0.20070723094225.04776510-at-pop.vt.edu} 3, 20 -- X-Sender: stmccart-at-pop.vt.edu 3, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 3, 20 -- Date: Mon, 23 Jul 2007 09:46:58 -0400 3, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 20 -- From: Stephen McCartney {stmccart-at-vt.edu} 3, 20 -- Subject: web based instrument sign up 3, 20 -- Mime-Version: 1.0 3, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
At the ANL EM Center we use a commerical web based calendar system for all our instruments, called Calcium. http://www.brownbearsw.com/
It is not designed for microscopy, but it works quite well for our needs.
You can see the on-line version of how we use this at the following URL
http://emc.msd.anl.gov/cgi-bin/Calcium39.pl
Since you do not have an account all you can do is view the calendar.
Neither I nor ANL have any financial interests in this software, other than being users.
Nestor Your Friendly Neighborhood SysOp
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Lab Electron Microscopy Center Materials Science Division/Bldg 212 9700 S. Cass Ave Argonne, Illinois 60439 USA Tel: 630-252-7901, Fax: 630-252-4798 Email: Zaluzec-at-aaem.amc.anl.gov =========================================== TPMLab: http://tpm.amc.anl.gov MMSite: http://www.amc.anl.gov ===========================================
The box said ... "This program requires Win 95/98/NT or better..." So I bought a Mac !
===========================================
==============================Original Headers============================== 15, 16 -- From zaluzec-at-aaem.amc.anl.gov Mon Jul 23 09:13:16 2007 15, 16 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3]) 15, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6NEDGGb013962 15, 16 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 09:13:16 -0500 15, 16 -- Received: from [146.139.72.105] (aem105.amc.anl.gov [146.139.72.105]) 15, 16 -- by aaem.amc.anl.gov (8.12.11.20060308/8.12.10) with ESMTP id l6NEDDYI009520 15, 16 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 09:13:14 -0500 15, 16 -- Mime-Version: 1.0 15, 16 -- Message-Id: {p06240804c2ca65d526a9-at-[146.139.72.105]} 15, 16 -- In-Reply-To: {200707231347.l6NDlCuv001518-at-ns.microscopy.com} 15, 16 -- References: {200707231347.l6NDlCuv001518-at-ns.microscopy.com} 15, 16 -- Date: Mon, 23 Jul 2007 09:13:11 -0500 15, 16 -- To: microscopy-at-microscopy.com 15, 16 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} 15, 16 -- Subject: Re: [Microscopy] web based instrument sign up 15, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Does anyone know of a calendar program appropriate for a microscopy lab that will run on the MAC?
Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
} From: {zaluzec-at-aaem.amc.anl.gov} } Reply-To: {zaluzec-at-aaem.amc.anl.gov} } Date: Mon, 23 Jul 2007 09:17:00 -0500 } To: {dsherman-at-purdue.edu} } Subject: [Microscopy] Re: web based instrument sign up } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Stephen } } At the ANL EM Center we use a commerical web based calendar system } for all our instruments, called Calcium. http://www.brownbearsw.com/ } } It is not designed for microscopy, but it works quite well for our needs. } } You can see the on-line version of how we use this at the following URL } } http://emc.msd.anl.gov/cgi-bin/Calcium39.pl } } Since you do not have an account all you can do is view the calendar. } } Neither I nor ANL have any financial interests in this software, other } than being users. } } Nestor } Your Friendly Neighborhood SysOp } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hello: We are going to a web based sign up for a new characterization } } facility that will contain about 10 instruments. We have done a search of } } the archives and found 3 systems, FACES and one from Northwestern and U. of } } Arizona. Are there others that people are using we should } } consider. Thanks. Steve } } } } Stephen McCartney } } Senior Research Associate } } Nanoscale Characterization and Fabrication Laboratory (NCFL) } } Institute for Critical Technology and Applied Science (ICTAS) } } 1991 Kraft Drive } } Va Tech } } Blacksburg, VA 24061 } } 540-231-9765 } } } } } } ==============================Original Headers============================== } } 3, 20 -- From stmccart-at-vt.edu Mon Jul 23 08:47:11 2007 } } 3, 20 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu } } [198.82.162.213]) } } 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id l6NDlBen001511 } } 3, 20 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 } } 08:47:11 -0500 } } 3, 20 -- Received: from vivi.cc.vt.edu } } (IDENT:mirapoint-at-evil-vivi.cc.vt.edu [10.1.1.12]) } } 3, 20 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with } } ESMTP id l6NDlAZL019144 } } 3, 20 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 } } 09:47:10 -0400 } } 3, 20 -- Received: from mri-system.vt.edu ([198.82.148.26]) } } 3, 20 -- by vivi.cc.vt.edu (MOS 3.8.2-GA) } } 3, 20 -- with ESMTP id HTD69749; } } 3, 20 -- Mon, 23 Jul 2007 09:47:10 -0400 (EDT) } } 3, 20 -- Message-Id: {5.2.1.1.0.20070723094225.04776510-at-pop.vt.edu} } } 3, 20 -- X-Sender: stmccart-at-pop.vt.edu } } 3, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 } } 3, 20 -- Date: Mon, 23 Jul 2007 09:46:58 -0400 } } 3, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} } } 3, 20 -- From: Stephen McCartney {stmccart-at-vt.edu} } } 3, 20 -- Subject: web based instrument sign up } } 3, 20 -- Mime-Version: 1.0 } } 3, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } } ==============================End of - Headers============================== } } } -- } =========================================== } Dr. Nestor J. Zaluzec } Argonne National Lab } Electron Microscopy Center } Materials Science Division/Bldg 212 } 9700 S. Cass Ave } Argonne, Illinois 60439 USA } Tel: 630-252-7901, Fax: 630-252-4798 } Email: Zaluzec-at-aaem.amc.anl.gov } =========================================== } TPMLab: http://tpm.amc.anl.gov } MMSite: http://www.amc.anl.gov } =========================================== } } The box said ... } "This program requires Win 95/98/NT or better..." } So I bought a Mac ! } } =========================================== } } ==============================Original Headers============================== } 15, 16 -- From zaluzec-at-aaem.amc.anl.gov Mon Jul 23 09:13:16 2007 } 15, 16 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3]) } 15, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l6NEDGGb013962 } 15, 16 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 09:13:16 -0500 } 15, 16 -- Received: from [146.139.72.105] (aem105.amc.anl.gov } [146.139.72.105]) } 15, 16 -- by aaem.amc.anl.gov (8.12.11.20060308/8.12.10) with ESMTP id } l6NEDDYI009520 } 15, 16 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 09:13:14 -0500 } 15, 16 -- Mime-Version: 1.0 } 15, 16 -- Message-Id: {p06240804c2ca65d526a9-at-[146.139.72.105]} } 15, 16 -- In-Reply-To: {200707231347.l6NDlCuv001518-at-ns.microscopy.com} } 15, 16 -- References: {200707231347.l6NDlCuv001518-at-ns.microscopy.com} } 15, 16 -- Date: Mon, 23 Jul 2007 09:13:11 -0500 } 15, 16 -- To: microscopy-at-microscopy.com } 15, 16 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} } 15, 16 -- Subject: Re: [Microscopy] web based instrument sign up } 15, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 23 -- From dsherman-at-purdue.edu Mon Jul 23 09:56:43 2007 6, 23 -- Received: from 1061exfe01.adpc.purdue.edu (1061exfe01.adpc.purdue.edu [128.210.63.221]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6NEuhsT026729 6, 23 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 09:56:43 -0500 6, 23 -- Received: from exchange.purdue.edu ([172.21.6.23]) by 1061exfe01.adpc.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 6, 23 -- Mon, 23 Jul 2007 10:56:43 -0400 6, 23 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 6, 23 -- Mon, 23 Jul 2007 14:56:43 +0000 6, 23 -- User-Agent: Microsoft-Entourage/11.3.6.070618 6, 23 -- Date: Mon, 23 Jul 2007 10:56:41 -0400 6, 23 -- Subject: Re: [Microscopy] Re: web based instrument sign up 6, 23 -- From: Debby Sherman {dsherman-at-purdue.edu} 6, 23 -- To: {zaluzec-at-aaem.amc.anl.gov} , 6, 23 -- "message to: MSA list" {microscopy-at-microscopy.com} 6, 23 -- Message-ID: {C2CA39E9.1F7B3%dsherman-at-purdue.edu} 6, 23 -- Thread-Topic: [Microscopy] Re: web based instrument sign up 6, 23 -- Thread-Index: AcfNOa1w67bV5DksEdyWtwARJN08Mg== 6, 23 -- In-Reply-To: {200707231417.l6NEH085017612-at-ns.microscopy.com} 6, 23 -- Mime-version: 1.0 6, 23 -- Content-type: text/plain; 6, 23 -- charset="US-ASCII" 6, 23 -- Content-transfer-encoding: 7bit 6, 23 -- X-OriginalArrivalTime: 23 Jul 2007 14:56:43.0359 (UTC) FILETIME=[AED84EF0:01C7CD39] ==============================End of - Headers==============================
We also wanted to move from a paper based reservation system to an online one. The calcium program that Nestor and others have mentioned is used by a lot of satisfied customers, and we almost became one of them. It was at the top of our list for purchase.
When we discussed the issues surrounding installation and maintenance of such a program with our network services staff, we found that we already had an online calendar on campus that might do what we wanted. It is part of the Banner suite of software packages from SCT, and includes the campus-wide finance, student records and campus portal system. The portal has a calendar function that we could use.
Calendars for each instrument can be created by the portal administrator, and then maintenance is up to people in the lab. Maintenance is simple and straight forward and is web based. Signup is also web based, so checking instrument availability and signup can be done from most anywhere. One of the vendor's intended uses of this calendar is to set up meetings, so signup consists of what amounts to setting up a meeting between yourself and an instrument. Also, signup requires login privileges to the campus portal system.
This is a simple system and does not have some features that might be important to some labs, like logging of changes, restrictions to specific times, maximum signup time allowed, etc. It simply replaced our paper calendars, which were pretty low tech. It was also already available, and, therefore, free.
--John John Chandler, Ph.D. Manager, Electron Microscopy Lab Colorado School of Mines 1500 Illinois Street Golden, CO 80401-1887 jpchandl-at-mines.edu 303.384.2203
-----Original Message----- X-from: zaluzec-at-aaem.amc.anl.gov [mailto:zaluzec-at-aaem.amc.anl.gov] Sent: Monday, July 23, 2007 8:20 AM To: jpchandl-at-mines.edu
Stephen
At the ANL EM Center we use a commerical web based calendar system for all our instruments, called Calcium. http://www.brownbearsw.com/
It is not designed for microscopy, but it works quite well for our needs.
You can see the on-line version of how we use this at the following URL
http://emc.msd.anl.gov/cgi-bin/Calcium39.pl
Since you do not have an account all you can do is view the calendar.
Neither I nor ANL have any financial interests in this software, other than being users.
Nestor Your Friendly Neighborhood SysOp
} --------------------------------------------------------------------------- - } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 22, 21 -- From jpchandl-at-mines.edu Mon Jul 23 10:32:50 2007 22, 21 -- Received: from incantation.Mines.EDU (incantation.Mines.EDU [138.67.130.6]) 22, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6NFWnFW018772 22, 21 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 10:32:50 -0500 22, 21 -- Received: from emlab1 (emlab1.Mines.EDU [138.67.28.173]) 22, 21 -- by incantation.Mines.EDU (8.13.1/8.13.1) with ESMTP id l6NFWmrJ022362 22, 21 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 09:32:49 -0600 22, 21 -- From: "John Chandler" {jpchandl-at-mines.edu} 22, 21 -- To: {microscopy-at-microscopy.com} 22, 21 -- References: {200707231420.l6NEKPZc023433-at-ns.microscopy.com} 22, 21 -- Subject: RE: [Microscopy] Re: web based instrument sign up 22, 21 -- Date: Mon, 23 Jul 2007 09:32:48 -0600 22, 21 -- Message-ID: {001501c7cd3e$b98e36c0$ad1c438a-at-mines.edu} 22, 21 -- MIME-Version: 1.0 22, 21 -- Content-Type: text/plain; 22, 21 -- charset="us-ascii" 22, 21 -- Content-Transfer-Encoding: 7bit 22, 21 -- X-Mailer: Microsoft Office Outlook 11 22, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 22, 21 -- Thread-Index: AcfNNJ464M0dNUzATfuAC6ByY/UcuAABVYTg 22, 21 -- In-Reply-To: {200707231420.l6NEKPZc023433-at-ns.microscopy.com} ==============================End of - Headers==============================
The calendar function in Groupwise can also be used to reserve equipment. We don't use it for the 'scopes, but the shared real-time PCR machines are scheduled this way. If it is already in place at your institution, you'd be ready to roll.
Tamara
On Mon, 23 Jul 2007 08:50:01 -0500 stmccart-at-vt.edu wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello: We are going to a web based sign up for a new } characterization } facility that will contain about 10 instruments. We } have done a search of } the archives and found 3 systems, FACES and one from } Northwestern and U. of } Arizona. Are there others that people are using we } should } consider. Thanks. Steve } } Stephen McCartney } Senior Research Associate } Nanoscale Characterization and Fabrication Laboratory } (NCFL) } Institute for Critical Technology and Applied Science } (ICTAS) } 1991 Kraft Drive } Va Tech } Blacksburg, VA 24061 } 540-231-9765 } } } ==============================Original } Headers============================== } 3, 20 -- From stmccart-at-vt.edu Mon Jul 23 08:47:11 2007 } 3, 20 -- Received: from lennier.cc.vt.edu } (lennier.cc.vt.edu [198.82.162.213]) } 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id l6NDlBen001511 } 3, 20 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul } 2007 08:47:11 -0500 } 3, 20 -- Received: from vivi.cc.vt.edu } (IDENT:mirapoint-at-evil-vivi.cc.vt.edu [10.1.1.12]) } 3, 20 -- by lennier.cc.vt.edu } (8.12.11.20060308/8.12.11) with ESMTP id l6NDlAZL019144 } 3, 20 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul } 2007 09:47:10 -0400 } 3, 20 -- Received: from mri-system.vt.edu } ([198.82.148.26]) } 3, 20 -- by vivi.cc.vt.edu (MOS 3.8.2-GA) } 3, 20 -- with ESMTP id HTD69749; } 3, 20 -- Mon, 23 Jul 2007 09:47:10 -0400 (EDT) } 3, 20 -- Message-Id: } {5.2.1.1.0.20070723094225.04776510-at-pop.vt.edu} } 3, 20 -- X-Sender: stmccart-at-pop.vt.edu } 3, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 } 3, 20 -- Date: Mon, 23 Jul 2007 09:46:58 -0400 } 3, 20 -- To: Microscopy Listserver } {microscopy-at-microscopy.com} } 3, 20 -- From: Stephen McCartney {stmccart-at-vt.edu} } 3, 20 -- Subject: web based instrument sign up } 3, 20 -- Mime-Version: 1.0 } 3, 20 -- Content-Type: text/plain; charset="us-ascii"; } format=flowed } ==============================End of - } Headers==============================
We are in the process of upgrading our microscopes and are out of space. The scopes listed bellow are available for sale.
*-------------------------------------------------------------------------------------------------* Images of the scopes can be downloaded (PDF) at:
http://www.3dfs.com/scopes_BioWarn.pdf
-- If you are having a hard time viewing the pdf, please email me: toli-at-hillweb.com* -------------------------------------------------------------------------------------------------* * * *Jeol JSM-35C* Working SEM, has been used as digital scope with A/D card (not included). Includes Link Analytical backscattering detector. 12k OBO
*ISI-40 SEM* Working condition 9k OBO ------------------------------------------------------------------------------------------------- * **Jeol 100-S TEM* Lots of work done to restore machine, but has not been turned on recently 5k OBO
- Microscopes professionally maintained. - Microscopes located in Pittsboro North Carolina, USA - We have means for professionally shutting down/ packaging and shipping the scopes.
------------------------------------------------------------------------------------------------- *For technical questions please contact:* Dr. Wolfgang Wagner, Director of microscopy 919-542-7410 wagner-at-hillweb.com* * *For sales please contact:* ¦ Anatoli Oleynik, Research Associate - BioWarn, LLC. ¦ 964 East St. Suite 106a - Pittsboro, NC 27312 ¦ O: 919.542.7410 | M: 919.260.1911 | F: 919.542.0268 ¦ mobile email/MSN-M: cell.toli-at-hotmail.com ¦ www.biowarnllc.com * *
Our University maintains a web-based group meeting calender, MeetingMaker, which we have used for about 3 years without a problem. (I don't have a financial interest in this software or company)
The advantages of this setup are it is very easy to use, and the University does the housekeeping chores vis-a-vis security, hackers, updates, etc.
The disadvantage is the system is only useful for reserving time on equipment. It isn't useful for linking to the equipment and automatically recording actual user time on equipment, assimilating the information on some sort of weekly or monthly schedule to a common location, making and posting billing, then collecting the money.
I know a number of us would like a system that does more than just reserve time on a calender.
Steve
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- Dr. Steven Barlow EM Facility/Biology Dept. San Diego State University 5500 Campanile Drive San Diego CA 92182-4614 phone: (619) 594-4523 fax: (619) 594-5676
Our vintage ISI WB-6 has suffered what appears to be a catastrophic failure.
It can't seem to focus in the high mag range. Everything acts OK, but the image will not focus, either autofocus or manual.
Our indy service guy has taken a look and thinks it might be a short in the obj lens, requiring a major dismantle to get in there to check it, and if it is, then what?. With no access to anything like factory service, does anyone have any ideas?
My dilemma is that I have a Hitachi S-2700 that I picked up for free waiting to be installed. Not sure if the Hitachi will work, was not operational when I picked it up, but it looks almost brand new. Price for the repair or the new install will be about the same. Which of course, is money that I don't have right now, but I am trying to plan ahead.
Before declaring the ISI a total loss and rolling it into the dumpster, anyone have a suggestion about a fix?
Thanks
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. Visit http://www.aidslifecycle.org for more information about the ride or http://www.aidslifecycle.org/5482 to make a donation.
==============================Original Headers============================== 10, 17 -- From jmkrupp-at-ucsc.edu Mon Jul 23 12:55:36 2007 10, 17 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6NHtaqB004618 10, 17 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 12:55:36 -0500 10, 17 -- Received: from [128.114.125.2] (HELO ucsc.edu) 10, 17 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 10, 17 -- with ESMTPS id 21313668 for microscopy-at-microscopy.com; Mon, 23 Jul 2007 10:55:35 -0700 10, 17 -- Received: from [128.114.25.127] (account jmkrupp-at-ucsc.edu HELO [128.114.25.127]) 10, 17 -- by silver.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 10, 17 -- with ESMTPA id 142236234 for microscopy-at-microscopy.com; Mon, 23 Jul 2007 10:55:35 -0700 10, 17 -- Mime-Version: 1.0 10, 17 -- Message-Id: {p06230902c2ca99089a1c-at-[128.114.25.127]} 10, 17 -- Date: Mon, 23 Jul 2007 10:55:34 -0700 10, 17 -- To: microscopy-at-microscopy.com 10, 17 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 10, 17 -- Subject: ISI WB-6 repair? 10, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Has anyone played around with using Google Calendar for scheduling instrument time? I use it to manage my daily agenda, but don't have a need (yet) to schedule instrument time, but it looks like a possible solution. I am not at all familiar with any other scheduling programs so Google Calendar may not be advanced enough, but since it's free and works with pretty much any browser it might be a solution.
-- ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Bryan Bandli Research Microscopist MVA Scientific Consultants 3300 Breckinridge Blvd., Suite 400 (770) 662-8509 bbandli-at-mvainc.com www.mvainc.com ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- The information in this email is confidential and may be legally privileged. It is intended solely for the addressee. If you are not the intended recipient, please delete the email and notify MVA Scientific Consultants of the transmission error. MVA Scientific Consultants - 3300 Breckinridge Blvd. Suite 400, Duluth, GA 30096 - (770)662-8509 -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
==============================Original Headers============================== 6, 20 -- From bbandli-at-mvainc.com Mon Jul 23 13:46:15 2007 6, 20 -- Received: from smtp02.atlngahp.sys.nuvox.net (smtp-out2.atlngahp.sys.nuvox.net [70.43.63.19]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6NIkEOe017660 6, 20 -- for {Microscopy-at-Microscopy.Com} ; Mon, 23 Jul 2007 13:46:15 -0500 6, 20 -- Received: from [192.168.1.95] (216.215.228.34.nw.nuvox.net [216.215.228.34]) 6, 20 -- by smtp02.atlngahp.sys.nuvox.net (8.13.1/8.13.1) with ESMTP id l6NIkEjE011044 6, 20 -- for {Microscopy-at-Microscopy.Com} ; Mon, 23 Jul 2007 14:46:14 -0400 6, 20 -- Message-ID: {46A4F8A5.2020607-at-mvainc.com} 6, 20 -- Date: Mon, 23 Jul 2007 14:51:17 -0400 6, 20 -- From: bbandli {bbandli-at-mvainc.com} 6, 20 -- Reply-To: bbandli-at-mvainc.com 6, 20 -- Organization: MVA Scientific Consultants 6, 20 -- User-Agent: Thunderbird 1.5.0.12 (Windows/20070509) 6, 20 -- MIME-Version: 1.0 6, 20 -- To: Microscopy-at-Microscopy.Com 6, 20 -- Subject: Re: [Microscopy] Re: web based instrument sign up 6, 20 -- References: {200707231726.l6NHQPlO016181-at-ns.microscopy.com} 6, 20 -- In-Reply-To: {200707231726.l6NHQPlO016181-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi, we also use the google calendar for keeping track of what is going on within the lab - courses, staff movements, seminars etc, but for instrument booking and simple usage records we use a home-grown SQL-based system - it was made several years ago by Nathan Cattle, an engineering student now in IT support, which is handy...we can get upgrades and improvements from time to time. We have users outside our institution, so can't use an institution-based system.
You can see a users-eye view of some equipment (SEMs) by going to http://www.anu.edu.au/EMU/booking/ and using DisplayS as both login and password. DisplayL , DisplayT..you get the picture. These are dummy users set up for convenience..but please dont actually make a booking!
Administrators (anyone can be set up temporarily as an administrator)can quickly set up new users with varying levels of access, new departments, and new instruments. Instruments have a range of usage patterns, and maximum bookable times. Major accessories or configuration options can be flagged. Staff concerned with an instrument optionally receive emails when a booking is made, with any comments entered by the user. Users tagged as "needing assistance" can easily be contacted to confirm or refuse bookings. Staff have a list of bookings for which they are responsible on their home page.
Stats of individual bookings (not filament time) are stored and can be accessed by instrument and month..this might be extended later. Users only see items they can access.
It works well for us, for about 25 instruments, and other departments have piggy-backed on the system for another 15 or so. This works without cluttering the screens because most users and administrators are not shown anything they dont need. So a molecular biology lab down the hall or in another building can operate effectively as an independent system.
Contact Nathan if you want to know more.
On a related note, we have just launched DOSSER http://dosser.anu.edu.au/index.php, a directory for "accessible" equipment. You dont need to sign up to browse or search it, only to add new equipment. Comments welcome!
cheers Sally
Dr SJ Stowe Facility Coordinator ANU Electron Microscopy Unit www.anu.edu.au/EMU/
On Mon, July 23, 2007 11:47 pm, stmccart-at-vt.edu wrote: }
} } } ------------------------------------------------------------------------- } --- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------- } -- } } } Hello: We are going to a web based sign up for a new characterization } facility that will contain about 10 instruments. We have done a search of } the archives and found 3 systems, FACES and one from Northwestern and U. } of Arizona. Are there others that people are using we should } consider. Thanks. Steve } } Stephen McCartney } Senior Research Associate } Nanoscale Characterization and Fabrication Laboratory (NCFL) } Institute for Critical Technology and Applied Science (ICTAS) } 1991 Kraft Drive } Va Tech } Blacksburg, VA 24061 } 540-231-9765 }
==============================Original Headers============================== 18, 27 -- From sally.stowe-at-anu.edu.au Mon Jul 23 17:35:31 2007 18, 27 -- Received: from mail.rsbs.anu.edu.au (mail.rsbs.anu.edu.au [150.203.72.70]) 18, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6NMZUFi014128 18, 27 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 17:35:31 -0500 18, 27 -- Received: from mail.rsbs.anu.edu.au (mail.rsbs.anu.edu.au [127.0.0.1]) 18, 27 -- by mail.rsbs.anu.edu.au (Postfix) with ESMTP 18, 27 -- id 607AFF441A0; Tue, 24 Jul 2007 08:35:23 +1000 (EST) 18, 27 -- Received: from 203.113.233.156 18, 27 -- (SquirrelMail authenticated user u8411377) 18, 27 -- by mail.rsbs.anu.edu.au with HTTP; 18, 27 -- Tue, 24 Jul 2007 08:35:23 +1000 (EST) 18, 27 -- Message-ID: {1308.203.113.233.156.1185230123.squirrel-at-mail.rsbs.anu.edu.au} 18, 27 -- In-Reply-To: {200707231347.l6NDlLpV001661-at-ns.microscopy.com} 18, 27 -- References: {200707231347.l6NDlLpV001661-at-ns.microscopy.com} 18, 27 -- Date: Tue, 24 Jul 2007 08:35:23 +1000 (EST) 18, 27 -- Subject: Re: [Microscopy] web based instrument sign up, calendar, 18, 27 -- DOSSER for shared equipment 18, 27 -- From: Sally.Stowe-at-anu.edu.au 18, 27 -- To: stmccart-at-vt.edu, microscopy-at-microscopy.com 18, 27 -- Reply-To: sally.stowe-at-anu.edu.au 18, 27 -- User-Agent: SquirrelMail/1.5.1 18, 27 -- MIME-Version: 1.0 18, 27 -- Content-Type: text/plain;charset=iso-8859-1 18, 27 -- Content-Transfer-Encoding: 8bit 18, 27 -- X-RSBS-MailScanner-Information: Please contact the ISP for more information 18, 27 -- X-RSBS-MailScanner: Found to be clean 18, 27 -- X-MailScanner-From: sally.stowe-at-anu.edu.au ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (dang-at-oakland.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, July 23, 2007 at 13:42:20 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both dang-at-oakland.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: dang-at-oakland.edu Name: LOAN DANG
Organization: Oakland University
Education: Graduate College
Location: Rochester Hills michigan 48309
Title: METHOD TO PROCESS THE LEAF FOR TEM
Question: I would like to have a protocol to fix the leaf and process it for the TEM Thank you LOAN DANG
The advantages of a dedicated software over general web calendar systems are:
1. It allows convenient tracking of instrument usage/repair history. 2. It gives statistical reports by departments, by PIs, or by instruments, which are important for funding applications and beyond. 3. It generates billing spreadsheet with several mouse clicks only. 4. It usually integrates solid access controls (remote relays) to the instruments, so users cannot use unauthorized instruments without logging in.
All these functions are critical for a recharge facility. However for simple share of instruments without accounting needs, web calendar system should be sufficient.
Shuyou _____________________________ Shuyou Li, Ph.D. NUANCE Center Northwestern University 2220 Campus Drive, 2036 Cook Hall Evanston, IL 60208-3108, USA Ph: (847) 491-6723 Fax: (847) 467-6573 Email: syli-at-northwestern.edu http://www.nuance.northwestern.edu/
-----Original Message----- X-from: Sally.Stowe-at-anu.edu.au [mailto:Sally.Stowe-at-anu.edu.au] Sent: Monday, July 23, 2007 5:42 PM To: syli-at-northwestern.edu
Hi, we also use the google calendar for keeping track of what is going on within the lab - courses, staff movements, seminars etc, but for instrument booking and simple usage records we use a home-grown SQL-based system - it was made several years ago by Nathan Cattle, an engineering student now in IT support, which is handy...we can get upgrades and improvements from time to time. We have users outside our institution, so can't use an institution-based system.
You can see a users-eye view of some equipment (SEMs) by going to http://www.anu.edu.au/EMU/booking/ and using DisplayS as both login and password. DisplayL , DisplayT..you get the picture. These are dummy users set up for convenience..but please dont actually make a booking!
Administrators (anyone can be set up temporarily as an administrator)can quickly set up new users with varying levels of access, new departments, and new instruments. Instruments have a range of usage patterns, and maximum bookable times. Major accessories or configuration options can be flagged. Staff concerned with an instrument optionally receive emails when a booking is made, with any comments entered by the user. Users tagged as "needing assistance" can easily be contacted to confirm or refuse bookings. Staff have a list of bookings for which they are responsible on their home page.
Stats of individual bookings (not filament time) are stored and can be accessed by instrument and month..this might be extended later. Users only see items they can access.
It works well for us, for about 25 instruments, and other departments have piggy-backed on the system for another 15 or so. This works without cluttering the screens because most users and administrators are not shown anything they dont need. So a molecular biology lab down the hall or in another building can operate effectively as an independent system.
Contact Nathan if you want to know more.
On a related note, we have just launched DOSSER http://dosser.anu.edu.au/index.php, a directory for "accessible" equipment. You dont need to sign up to browse or search it, only to add new equipment. Comments welcome!
cheers Sally
Dr SJ Stowe Facility Coordinator ANU Electron Microscopy Unit www.anu.edu.au/EMU/
On Mon, July 23, 2007 11:47 pm, stmccart-at-vt.edu wrote: }
} } } ---------------------------------------------------------------------- } --- } --- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ---- } -- } } } Hello: We are going to a web based sign up for a new characterization } facility that will contain about 10 instruments. We have done a } search of the archives and found 3 systems, FACES and one from Northwestern and U. } of Arizona. Are there others that people are using we should } consider. Thanks. Steve } } Stephen McCartney } Senior Research Associate } Nanoscale Characterization and Fabrication Laboratory (NCFL) Institute } for Critical Technology and Applied Science (ICTAS) } 1991 Kraft Drive } Va Tech } Blacksburg, VA 24061 } 540-231-9765 }
==============================Original Headers============================== 18, 27 -- From sally.stowe-at-anu.edu.au Mon Jul 23 17:35:31 2007 18, 27 -- Received: from mail.rsbs.anu.edu.au (mail.rsbs.anu.edu.au [150.203.72.70]) 18, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6NMZUFi014128 18, 27 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 17:35:31 -0500 18, 27 -- Received: from mail.rsbs.anu.edu.au (mail.rsbs.anu.edu.au [127.0.0.1]) 18, 27 -- by mail.rsbs.anu.edu.au (Postfix) with ESMTP 18, 27 -- id 607AFF441A0; Tue, 24 Jul 2007 08:35:23 +1000 (EST) 18, 27 -- Received: from 203.113.233.156 18, 27 -- (SquirrelMail authenticated user u8411377) 18, 27 -- by mail.rsbs.anu.edu.au with HTTP; 18, 27 -- Tue, 24 Jul 2007 08:35:23 +1000 (EST) 18, 27 -- Message-ID: {1308.203.113.233.156.1185230123.squirrel-at-mail.rsbs.anu.edu.au} 18, 27 -- In-Reply-To: {200707231347.l6NDlLpV001661-at-ns.microscopy.com} 18, 27 -- References: {200707231347.l6NDlLpV001661-at-ns.microscopy.com} 18, 27 -- Date: Tue, 24 Jul 2007 08:35:23 +1000 (EST) 18, 27 -- Subject: Re: [Microscopy] web based instrument sign up, calendar, 18, 27 -- DOSSER for shared equipment 18, 27 -- From: Sally.Stowe-at-anu.edu.au 18, 27 -- To: stmccart-at-vt.edu, microscopy-at-microscopy.com 18, 27 -- Reply-To: sally.stowe-at-anu.edu.au 18, 27 -- User-Agent: SquirrelMail/1.5.1 18, 27 -- MIME-Version: 1.0 18, 27 -- Content-Type: text/plain;charset=iso-8859-1 18, 27 -- Content-Transfer-Encoding: 8bit 18, 27 -- X-RSBS-MailScanner-Information: Please contact the ISP for more information 18, 27 -- X-RSBS-MailScanner: Found to be clean 18, 27 -- X-MailScanner-From: sally.stowe-at-anu.edu.au ==============================End of - Headers==============================
==============================Original Headers============================== 31, 21 -- From syli-at-northwestern.edu Mon Jul 23 18:46:15 2007 31, 21 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57]) 31, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6NNkEp8006532 31, 21 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 18:46:14 -0500 31, 21 -- Received: from prep1 (ms48.ms.northwestern.edu [129.105.37.48]) 31, 21 -- by merle.it.northwestern.edu (Postfix) with ESMTP id C440174B1 31, 21 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 18:46:14 -0500 (CDT) 31, 21 -- From: "Shuyou Li" {syli-at-northwestern.edu} 31, 21 -- To: {microscopy-at-microscopy.com} 31, 21 -- References: {200707232241.l6NMfZHh020973-at-ns.microscopy.com} 31, 21 -- Subject: RE: [Microscopy] Re: web based instrument sign up, calendar, 31, 21 -- Date: Mon, 23 Jul 2007 18:46:11 -0500 31, 21 -- Message-ID: {003101c7cd83$a67258b0$30256981-at-prep1} 31, 21 -- MIME-Version: 1.0 31, 21 -- Content-Type: text/plain; 31, 21 -- charset="us-ascii" 31, 21 -- Content-Transfer-Encoding: 7bit 31, 21 -- X-Mailer: Microsoft Office Outlook 11 31, 21 -- In-Reply-To: {200707232241.l6NMfZHh020973-at-ns.microscopy.com} 31, 21 -- Thread-Index: AcfNeqRTYaNhYRlES5ilhy9zEC9L8gABm4Yg 31, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 ==============================End of - Headers==============================
We are taking apart a Hitachi S-800 FESEM for disposal. We have scavenged some parts for another instrument, but some items are still available. If you are interested in anything, please email me (tina-at-pbrc.hawaii.edu) as soon as possible - it is coming apart within days!
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 5, 19 -- From tina-at-pbrc.hawaii.edu Mon Jul 23 21:54:43 2007 5, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6O2sgma021166 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jul 2007 21:54:43 -0500 5, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id l6O2scTN002216 5, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jul 2007 16:54:39 -1000 (HST) 5, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id l6O2sbLk002213 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jul 2007 16:54:38 -1000 (HST) 5, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 5, 19 -- Date: Mon, 23 Jul 2007 16:54:37 -1000 (HST) 5, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 19 -- X-Sender: tina-at-halia 5, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 5, 19 -- Subject: Parts available for S-800 5, 19 -- Message-ID: {Pine.GSO.4.21.0707231652100.2203-100000-at-halia} 5, 19 -- MIME-Version: 1.0 5, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
We use phpgroupware (http://www.phpgroupware.org/), for individual calendar and instrument reservation.
It's not the most "clik and play" developed software, but it is web-based (people work on mac, linux/unix and windows here), it's free and open, and based on php, one can recover all the data for use for statistics or billing.
We use it for all shared or service instruments (microscopes, x-ray diffractor, AFM, etc.), but for meeting rooms or things like video projector too.
It gives probably more achieved applications, but this one is enough for our needs,... and budget !
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
stmccart-at-vt.edu a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello: We are going to a web based sign up for a new characterization } facility that will contain about 10 instruments. We have done a search of } the archives and found 3 systems, FACES and one from Northwestern and U. of } Arizona. Are there others that people are using we should } consider. Thanks. Steve } } Stephen McCartney } Senior Research Associate } Nanoscale Characterization and Fabrication Laboratory (NCFL) } Institute for Critical Technology and Applied Science (ICTAS) } 1991 Kraft Drive } Va Tech } Blacksburg, VA 24061 } 540-231-9765 } } } ==============================Original Headers============================== } 3, 20 -- From stmccart-at-vt.edu Mon Jul 23 08:47:11 2007 } 3, 20 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu [198.82.162.213]) } 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6NDlBen001511 } 3, 20 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 08:47:11 -0500 } 3, 20 -- Received: from vivi.cc.vt.edu (IDENT:mirapoint-at-evil-vivi.cc.vt.edu [10.1.1.12]) } 3, 20 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id l6NDlAZL019144 } 3, 20 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jul 2007 09:47:10 -0400 } 3, 20 -- Received: from mri-system.vt.edu ([198.82.148.26]) } 3, 20 -- by vivi.cc.vt.edu (MOS 3.8.2-GA) } 3, 20 -- with ESMTP id HTD69749; } 3, 20 -- Mon, 23 Jul 2007 09:47:10 -0400 (EDT) } 3, 20 -- Message-Id: {5.2.1.1.0.20070723094225.04776510-at-pop.vt.edu} } 3, 20 -- X-Sender: stmccart-at-pop.vt.edu } 3, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 } 3, 20 -- Date: Mon, 23 Jul 2007 09:46:58 -0400 } 3, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} } 3, 20 -- From: Stephen McCartney {stmccart-at-vt.edu} } 3, 20 -- Subject: web based instrument sign up } 3, 20 -- Mime-Version: 1.0 } 3, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Jul 24 02:14:00 2007 9, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.152]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6O7Dx9i004895 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jul 2007 02:13:59 -0500 9, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 9, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id l6O7Dvc0007766 9, 29 -- ; Tue, 24 Jul 2007 09:13:58 +0200 (CEST) 9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id F34541068644; 9, 29 -- Tue, 24 Jul 2007 09:13:12 +0200 (CEST) 9, 29 -- Message-ID: {46A5A68F.6000205-at-ipcms.u-strasbg.fr} 9, 29 -- Date: Tue, 24 Jul 2007 09:13:19 +0200 9, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 9, 29 -- User-Agent: Thunderbird 1.5.0.12 (X11/20070604) 9, 29 -- MIME-Version: 1.0 9, 29 -- To: stmccart-at-vt.edu, Microscopy-at-microscopy.com 9, 29 -- Subject: Re: [Microscopy] web based instrument sign up 9, 29 -- References: {200707231355.l6NDtrjU010536-at-ns.microscopy.com} 9, 29 -- In-Reply-To: {200707231355.l6NDtrjU010536-at-ns.microscopy.com} 9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 29 -- Content-Transfer-Encoding: 8bit 9, 29 -- X-IPCMS-MailScanner: Found to be clean 9, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.152]); Tue, 24 Jul 2007 09:13:58 +0200 (CEST) 9, 29 -- X-Virus-Scanned: ClamAV 0.88.7/3750/Tue Jul 24 05:01:29 2007 on mr2.u-strasbg.fr 9, 29 -- X-Virus-Status: Clean 9, 29 -- X-Spam-Status: No, score=-0.2 required=5.0 tests=AWL autolearn=disabled 9, 29 -- version=3.1.8 9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr2.u-strasbg.fr ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mj_iqbal-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mj_iqbal-at-yahoo.com Name: Muhammad Javed
Title-Subject: [Filtered] Bacteria in Root Nodule
Question: Dear Randy I am of the mind the suggestion given by Pat is of great worth but I would like to add few more things .we are working on legume root nodule from so long and results are the excellent even for the immunogold labelling as well, where the section thickness, stability matters a great. I am forwarding you the protocol (on and off list) along with the tips that are common with a slight change. Discussion or details about the topic might be long and other people would not have an interest in that. If you still face some problem or having quires donÇt hesitate to write. Muhammad Javed Iqbal Electron Microscopist Transmission Electron Microscopy Facility TEM Labs Ph # +92-41-651475-80 Ext #264 {mailto:mj_iqbal-at-yahoo.com} mj_iqbal-at-yahoo.com {mailto:mjiqbal-at-nibge.org} mjiqbal-at-nibge.org NIBGE Pakistan
Protocol for Ultra Structural Studies Material: Pipes Buffer [0.2M] (Piperzine-N,N-bis [2-ethanesulfonic acid]) FW : 302 Add 6gm Pipes to 50 ml of distilled water. Add 1N NaOH drop wise until solid dissolves then adjust ph 6.8. Make up a volume of 100ml and store at 4 0 C. Fixative Add 5 ml of (5 % v/v) glutaraldeyhde to 10 ml of 0.2M Pipes buffer. Adjust ph 6.8.With 1N NaOH and make up volume to 25 ml. Lead Citrate [4%] Pb(C6H5O7). 3H2O FW: 1053.82 Add 0.04 g Lead citrate salt to 10 ml of double distilled and autoclaved water shake thoroughly then add 100 µl of 10 N NaOH solution. Prepare fresh before use. Make sure that Co2 present in the air should not trap with lead citrate solution which may lead to precipitates. Uranyl Acetatae [5%] UO2(CH3COO)2. 2H2O FW : 424.14 Dissolve 5 gm of Uranyl Acetate in 100 ml of distilled water (filter sterilize with 0.2m Cellulose Acetate filter and store in cuvettes covered with aluminum foil to save from direct light) used as stain to enhance electron density. Ethanol Series 30%, 50 %, 70 %, 100 % as dehydrating agent Acetone Absolute Acetone as transitional solvent Spur Resin. ERL / VCD 4206 (Vinylcyclohexene dioxide) 10 Parts DER736 (diglycidyl ether of propylene glycol) 6 NSA (Nonyl succnic anhydride) 26 S1 (Dimethylaminoethanol) 0.4 Methods: 1.5% w/v Difco water agar was prepared after boiling in a microwave oven and was kept in oven set at 37 0 C to prepare agar blocks. Small pieces almost of 1mm X 1mm size from fresh root nodules were (cut under a drop of fixative) were placed in 1.5% Difco agar that was poured in a Petri dish and was left for 10 minutes at room temperature after agar sets hard (solidifies). The agar (blocks) that were holding a thin layer of agar around the nodule tissue was cut with a sharp scalpel Blade. These blocks were immersed in 5%Glutaraldehyde prepared in 0.2 M Pipes Buffer and Ph was set to 6.8. These small pieces were subjected to vacuum in order to draw the fixative in to the tissue for half an hour. Fixation took place in 18 Hrs placing the samples on a 55 0 fixed angle specimen rotator at 5 rpm on room temperature. Rinsing of samples to remove the fixative was performed in .2M Pipes buffer Ph 6.8 for three times with an interval of 15 minutes each Post fixation was achieved with 1% Osmium Tetroxide for 18Hrs at room temperature The tissue was washed with autoclaved distilled water twice for 15 min and then shifted to 5% Uranyl Acetate solution for 16-18 Hrs The tissue was again washed with distilled waster twice for 15 min and then dehydrated with Ethanol series comprising of 30%, 50 %, 70 %, 100% dilutions consequently and kept in each fraction for 15 min and in 100 % thrice. 2 times for 1 hour and then before leaving overnight replace the ethanol with new one. The tissue was left in absolute Acetone 2 X 30 min as transitional solvent because the ethanol doesnÇt mixes well with spur Resin. Tissue was infiltrated with a mixture of Spur embedding media. The ratio of resin to acetone was as follows 1:3 , 1:2, 1:1 each for 8 hrs al least and then absolute resin 100% initially for 8 hrs and then left overnight .The samples were oriented in Moulds and resin cured at 60 0C for 48-60 Hrs. The polymerized resin blocks were trimmed like a trapezoid and faced with fine scalpel blade. Ultra thin serial sections of approx 120nm were made with RMC MT 7000 ultra micro tome and placed on 300 mesh nickel grid. Stained the grids with 5% Uranyl Acetate for 30 minutes and washed three times with distilled water. Grids were double stained with Lead Citrate for 10 minutes in NaOH chamber. Examination of sections was performed with JEOL JEM1010 Transmission Electron Microscope operating at 80 kv available at TEM Facilities Lab National Institute for Biotechnology & Genetic Engineering (NIBGE ) Faisalabad. Pakistan Plastic sections are enough stable that you would not need help of any plastic or carbon film Note: For mixing and infiltration the tissue was kept on specimen rotator All the steps were adopted at room temperature almost 24-26 0C
Does anybody have a tip on how to un-stick a vacuum pump? I have a JEOL JSM-35C that I'm getting up and running, and one of the pumps is bound. I can turn it by hand, but it takes a lot of effort. The motor spins, and is right now grinding away at the belt when it turns, since it lacks the torque necessary to turn the vacuum pump wheel.
What could cause it to bind up like that? It has enough oil in it, and the oil looks good.
Could be the seals or bearings, but it has been my experience that most often the sliding vanes and guides are damaged and/or sticking. I have repaired this type of problem, but it requires pump disassembly, cleaning, and parts replacement. Usually repair entails replacement of the vanes, guide pins and vane springs. It would be good to replace the shaft oil seals while you are at it.
If you can find a service manual for the pump it would be most helpful. For some pumps, it is not immediately obvious what to remove for disassembly. Furthermore, some require eccentric bearings be rotated to achieve the proper vane to housing clearances.
Another possibility... If the torque requirement is within specification (even though stiff) and the drive motor is a capacitor start type, a bad starting capacitor will cause failure to start. A bad capacitor will reduce the motor start torque to near zero.
Woody
NW (Woody) White Jr BWXT Services Lynchburg, VA http://www.bwxt.com/operations/semlab.html
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Tuesday, July 24, 2007 12:27 PM To: White, Woody N.
Does anybody have a tip on how to un-stick a vacuum pump? I have a JEOL JSM-35C that I'm getting up and running, and one of the pumps is bound. I can turn it by hand, but it takes a lot of effort. The motor spins, and is right now grinding away at the belt when it turns, since it lacks the torque necessary to turn the vacuum pump wheel.
What could cause it to bind up like that? It has enough oil in it, and the oil looks good.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (gma27824-at-csun.edu) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, July 24, 2007 at 13:31:24 ---------------------------------------------------------------------------
Organization: California State University, Northridge (CSUN)
Education: Graduate College
Location: Northridge, CA, USA
Question: I am about to be trained to use a TEM to take electronmicrographs of a bacterium called Arthrobacter GFB100 that might be producing a biofilm. As very few people work with it, I am looking at a protocol performed on a similar genus. I have two questions: 1)In the fixation step, OsO4 in Veronal-sodium acetate is used. What is veronal-sodium acetate, and why would it be used as opposed to more conventional fixation agents? 2)After polymerization, the sample was freed from a glass coverslip by shock-freezing in liquid nitrogen. Is this absolutely necessary or are there other alternatives? The paper was published by the Journal of Bacteriology, Jan. 1997, p.31-40, vol.179, No.1, entitled, "Metal Selectivity of In Situ Microcolonies in Biofilms of the Elbe River"; the authors are H. Lundsdorf, I. Brummer, K. N. Timmis, and I. Wagner-Dobler. Any information would be much appreciated-Thank you!
Does anybody have the pinouts and connection diagrams for the Kevex Delta-3 Xray analyzer? We received the full Kevex system with our donated JSM-35C, and I would like to be able to use it for imaging, but the cables were not included.
Oh, and for those who remember my employment situation, I've landed a much better position teaching forensics, physics, chemistry and astronomy at another school, still to high school. And the new school is much better about letting me be a little, um, non-traditional with my teaching methods. (More labs, less book.)
--Justin A. Kraft Leadership Academy West Palm Beach, FL.
Excuse me, but I think you need to spend some serious time working with the SEM system. Whatever it is. You had a Jeol system then an ISI system and now problems with EDS.
This is a bit too much, IMO. Free stuff has a price. There are no free lunches here.
Reality is a shocking wall of resistance. I do not mean to be combative or negative.
However, you need to be, IMO, more realistic in the scope of what you are trying to do. It is not trivial by any measure. But if you can prevail---all the better for you.
Stand back and prioritize your issues. If no beam, EDS is irrelevant. Get the SEM stable and then move forward.....my opinion.
Do you have a beam of quality???
gary g.
At 07:16 PM 7/24/2007, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Yes. It collects two microscopy listservs. I rarely look at it; your last incoming message was Feb '06. Contact via Exeter Road is best.
Gum
--- David Sanderson {David-at-SandersonsGarage.co.uk} wrote:
} Hi Gum, } } Are you still on this e-mail address. } } David }
___________________________________________________________ Yahoo! Answers - Got a question? Someone out there knows the answer. Try it now. http://uk.answers.yahoo.com/
==============================Original Headers============================== 9, 20 -- From jb_sanderson-at-yahoo.com Wed Jul 25 14:25:26 2007 9, 20 -- Received: from web56415.mail.re3.yahoo.com (web56415.mail.re3.yahoo.com [216.252.111.94]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l6PJPQgA006425 9, 20 -- for {microscopy-at-sparc5.microscopy.com} ; Wed, 25 Jul 2007 14:25:26 -0500 9, 20 -- Received: (qmail 15435 invoked by uid 60001); 25 Jul 2007 19:25:24 -0000 9, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 20 -- s=s1024; d=yahoo.com; 9, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 9, 20 -- b=dhYw7wJF1ZOje7vj3Kv843boz39HfUgOBO34w1OLg9pRKvRl03jMC/oCsLZOQlSji9xqQwgQUv0+2HJikKtCwac1gVQ3rMeyKyoGvhHRjKetjhhC84tAZC97zzwHIZnGR0ndB28CAJhYz5/rxHPTkHdznFqAT0hzdMe1OP/flQo=; 9, 20 -- X-YMail-OSG: 2tkVty4VM1mbD9O1PGIFfnreRMpl.7_HU4CrWT3N6eleescyZbQz6CXVOaxZiN_94J39GSLDbuzn.sk476rsXhUyvfN_9v2KqYd8SmvMtT1UHb3j2LFPX0Nqjz3i4Q-- 9, 20 -- Received: from [143.167.92.7] by web56415.mail.re3.yahoo.com via HTTP; Wed, 25 Jul 2007 12:25:24 PDT 9, 20 -- Date: Wed, 25 Jul 2007 12:25:24 -0700 (PDT) 9, 20 -- From: Jeremy Sanderson {jb_sanderson-at-yahoo.com} 9, 20 -- Subject: Re: Are you there 9, 20 -- To: david-at-sandersonsgarage.co.uk 9, 20 -- In-Reply-To: {MABBJAJDIJHINOMPIHMBOEDBDFAA.David-at-SandersonsGarage.co.uk} 9, 20 -- MIME-Version: 1.0 9, 20 -- Content-Type: text/plain; charset=iso-8859-1 9, 20 -- Content-Transfer-Encoding: 8bit 9, 20 -- Message-ID: {843705.8850.qm-at-web56415.mail.re3.yahoo.com} ==============================End of - Headers==============================
Veronal - sodium acetate is a buffer composed of sodium barbitol and sodium acetate. It was made popular by Ryter & Kellenberger many decades ago to preserve "bacterial plasms." Even now, I use it sometimes to fix stubborn organisms. However, it is a controlled substance (a barbiturate) and probably difficult to obtain without a license. With hard to fix organisms, I often fix overnight (at room temperature) in osmium tetroxide buffered in veronal acetate.
You should try a more conventional buffer (one of the phosphates, for example) for various times. Try following the protocol that you mention only use phosphate buffer and extend the times a bit.
The reason for LN2 shocking was to release the organisms. Unless there is a good reason for culturing on glass substrates, use a plastic petri dish instead (or a coverglass made of plastic or Aclar), and do a monolayer embedding. That way, you can examine the cells as they grow (in situ) on the plastic surface. I have a technique for doing this. If you are interested, I can dig it out.
JB
} Question: I am about to be trained to use a TEM to take } electronmicrographs of a bacterium called Arthrobacter GFB100 that } might be producing a biofilm. As very few people work with it, I am } looking at a protocol performed on a similar genus. I have two } questions: 1)In the fixation step, OsO4 in Veronal-sodium acetate is } used. What is veronal-sodium acetate, and why would it be used as } opposed to more conventional fixation agents? 2)After } polymerization, the sample was freed from a glass coverslip by } shock-freezing in liquid nitrogen. Is this absolutely necessary or } are there other alternatives? The paper was published by the Journal } of Bacteriology, Jan. 1997, p.31-40, vol.179, No.1, entitled, "Metal } Selectivity of In Situ Microcolonies in Biofilms of the Elbe River"; } the authors are H. Lundsdorf, I. Brummer, K. N. Timmis, and I. } Wagner-Dobler. Any information would be much appreciated-Thank you!
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Researcher gave me a sample of diatoms in 4% formaldehyde in sea water. She made a standard collection and used her usual formaldehyde fix since it was going to be for light microscopy and population monitoring only.
Well, some weird things showed up, now she wants to check them using TEM to see if there are any viruses inside or if she can figure out what else might be going on.
I, of course, have been called on to do the EM. So, I'm trying to work out something that makes sense. The guys are in 4% formaldehyde in sea water. Wondering about rinsing out SW before or after OsO4 and or choice of buffer, if needed.
We know these guys may have suffered from the formaldehyde and may not turn out so well, but figure its worth a try. Any hints?
Thanks
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. Visit http://www.aidslifecycle.org for more information about the ride or http://www.aidslifecycle.org/5482 to make a donation.
==============================Original Headers============================== 10, 17 -- From jmkrupp-at-ucsc.edu Wed Jul 25 15:40:18 2007 10, 17 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6PKeHH0031755 10, 17 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jul 2007 15:40:18 -0500 10, 17 -- Received: from [128.114.125.2] (HELO ucsc.edu) 10, 17 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 10, 17 -- with ESMTPS id 21524818 for microscopy-at-microscopy.com; Wed, 25 Jul 2007 13:40:13 -0700 10, 17 -- Received: from [128.114.25.127] (account jmkrupp-at-ucsc.edu HELO [128.114.25.127]) 10, 17 -- by silver.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 10, 17 -- with ESMTPA id 142855427 for microscopy-at-microscopy.com; Wed, 25 Jul 2007 13:40:12 -0700 10, 17 -- Mime-Version: 1.0 10, 17 -- Message-Id: {p06230903c2cd63682e9c-at-[128.114.25.127]} 10, 17 -- Date: Wed, 25 Jul 2007 13:40:11 -0700 10, 17 -- To: microscopy-at-microscopy.com 10, 17 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 10, 17 -- Subject: TEM after formaldehyde? 10, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I read a paper recently on using platinum blue as an alternative to Uranyl Acetate for en bloc and post section staining. Does anybody have the recipe for how to make it? I contacted one of the writers of the paper and the recipe I have is in japanese so if anyone has an english version I would greatly appreciate it.
Are you sure there is biological material inside the crusty diatoms?
I had done some work with diatoms by putting them whole onto coated grids but that was for identification, not to see the insides. I did get some lovely pictures and found one that was not known to be present in North America! We did not have a SEM back then - 1972 I think.
You can do a 1% glutaraldehyde fix in sea water (did that for marine animal sperm) then go into buffer (I used phosphate) to wash and osmium fix.
I would not wish to thin section these critters without some advice from someone who had done so successfully. I had a lot of trouble with sponges many years ago. The spicules(?) made a mess of the sections. This was before I joined the Listserv and did not know anyone to ask for help.
Try glass knives before diamond I would think.
Lost of luck, Pat
Patricia Stranen Connelly Lead Technologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 connellyps-at-mail.nih.gov
On 7/25/07 4:44 PM, "jmkrupp-at-ucsc.edu" {jmkrupp-at-ucsc.edu} wrote: } Just checking before I try something new. } } Researcher gave me a sample of diatoms in 4% formaldehyde in sea } water. She made a standard collection and used her usual formaldehyde } fix since it was going to be for light microscopy and population } monitoring only. } } Well, some weird things showed up, now she wants to check them using } TEM to see if there are any viruses inside or if she can figure out } what else might be going on. } } I, of course, have been called on to do the EM. So, I'm trying to } work out something that makes sense. The guys are in 4% formaldehyde } in sea water. Wondering about rinsing out SW before or after OsO4 and } or choice of buffer, if needed. } } We know these guys may have suffered from the formaldehyde and may } not turn out so well, but figure its worth a try. Any hints? } } Thanks } Jon
==============================Original Headers============================== 12, 24 -- From connellyps-at-nhlbi.nih.gov Wed Jul 25 18:38:33 2007 12, 24 -- Received: from NIHCESSMTP.hub.nih.gov (nihcessmtp.hub.nih.gov [128.231.90.115]) 12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l6PNcXCl012834 12, 24 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jul 2007 18:38:33 -0500 12, 24 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 12, 24 -- Wed, 25 Jul 2007 19:38:28 -0400 12, 24 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.169]) with Microsoft Exchange Server HTTP-DAV ; 12, 24 -- Wed, 25 Jul 2007 23:38:27 +0000 12, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618 12, 24 -- Date: Wed, 25 Jul 2007 19:37:26 -0400 12, 24 -- Subject: Re: [Microscopy] TEM after formaldehyde? 12, 24 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 12, 24 -- To: {jmkrupp-at-ucsc.edu} 12, 24 -- CC: {microscopy-at-microscopy.com} 12, 24 -- Message-ID: {C2CD56F6.94B%connellyps-at-nhlbi.nih.gov} 12, 24 -- Thread-Topic: [Microscopy] TEM after formaldehyde? 12, 24 -- Thread-Index: AcfPFMG8ACXgiDsIEdyQOgANk2Yv1A== 12, 24 -- In-Reply-To: {200707252044.l6PKiTb9007918-at-ns.microscopy.com} 12, 24 -- Mime-version: 1.0 12, 24 -- Content-type: text/plain; 12, 24 -- charset="ISO-8859-1" 12, 24 -- X-OriginalArrivalTime: 25 Jul 2007 23:38:28.0262 (UTC) FILETIME=[E6D8EC60:01C7CF14] 12, 24 -- Content-Transfer-Encoding: 8bit 12, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l6PNcXCl012834 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both opmills-at-mtu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: opmills-at-mtu.edu Name: Owen Mills
Organization: Michigan Tech Univ
Title-Subject: [Filtered] lab climate control
Question: All,
I'm curious whether any of you are using the rolling air conditioning units with a vent hose. If so, do they work? What size units are you using (in BTU's).
I also recommended (off list) flat embedding, however definitely not in a plastic dish! The reason is that the plastic dish is not perfectly flat, and when you flat embed the surface of the resin bloc is full of holes. This means you'll need 8-10 100 nm thick sections before obtaining a perfectly flat surface. This is probably the thickness where the bacteria are to be found! Of course you can still try to image your bacteria in sections full of holes, this is another story.
An alternative would be to actually grow on glass coverslips and flat embed in eppendorf tubes whose bottom has been cut out. The glass coverslips offer a perfectly flat surface, and if you approach the knife (well actually the spectimen) carefully enough, the first few sections may be ok. Other more expensive alternative exist (thermanox coverslips for example).
Best regards,
Stephane
--- bozzola-at-siu.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Veronal - sodium acetate is a buffer composed of } sodium barbitol and } sodium acetate. It was made popular by Ryter & } Kellenberger many } decades ago to preserve "bacterial plasms." Even } now, I use it } sometimes to fix stubborn organisms. However, it is } a controlled } substance (a barbiturate) and probably difficult to } obtain without a } license. With hard to fix organisms, I often fix } overnight (at room } temperature) in osmium tetroxide buffered in veronal } acetate. } } You should try a more conventional buffer (one of } the phosphates, for } example) for various times. Try following the } protocol that you } mention only use phosphate buffer and extend the } times a bit. } } The reason for LN2 shocking was to release the } organisms. Unless } there is a good reason for culturing on glass } substrates, use a } plastic petri dish instead (or a coverglass made of } plastic or } Aclar), and do a monolayer embedding. That way, you } can examine the } cells as they grow (in situ) on the plastic surface. } I have a } technique for doing this. If you are interested, I } can dig it out. } } JB } } } Question: I am about to be trained to use a TEM to } take } } electronmicrographs of a bacterium called } Arthrobacter GFB100 that } } might be producing a biofilm. As very few people } work with it, I am } } looking at a protocol performed on a similar genus. } I have two } } questions: 1)In the fixation step, OsO4 in } Veronal-sodium acetate is } } used. What is veronal-sodium acetate, and why would } it be used as } } opposed to more conventional fixation agents? } 2)After } } polymerization, the sample was freed from a glass } coverslip by } } shock-freezing in liquid nitrogen. Is this } absolutely necessary or } } are there other alternatives? The paper was } published by the Journal } } of Bacteriology, Jan. 1997, p.31-40, vol.179, No.1, } entitled, "Metal } } Selectivity of In Situ Microcolonies in Biofilms of } the Elbe River"; } } the authors are H. Lundsdorf, I. Brummer, K. N. } Timmis, and I. } } Wagner-Dobler. Any information would be much } appreciated-Thank you! } } } -- } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } John J. Bozzola, Ph.D., Director } Integrated Microscopy & Graphics Expertise (IMAGE) } Southern Illinois University } 750 Communications Drive - MC 4402 } Carbondale, IL 62901 } Telephone: 618-453-3730 } } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } ==============================Original } Headers============================== } 9, 17 -- From bozzola-at-siu.edu Wed Jul 25 15:16:27 } 2007 } 9, 17 -- Received: from abbmta1.siu.edu } (abbmta1.siu.edu [131.230.254.205]) } 9, 17 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l6PKGRPH019352 } 9, 17 -- for {Microscopy-at-msa.microscopy.com} ; Wed, } 25 Jul 2007 15:16:27 -0500 } 9, 17 -- Received: from [131.230.177.136] } (ws177136.microscope.siu.edu [131.230.177.136]) } 9, 17 -- by abbmta1.siu.edu } (Switch-3.2.5/Switch-3.2.5) with ESMTP id } l6PKGP4o004207 } 9, 17 -- for {Microscopy-at-msa.microscopy.com} ; Wed, } 25 Jul 2007 15:16:26 -0500 (CDT) } 9, 17 -- Mime-Version: 1.0 } 9, 17 -- Message-Id: } {a06240801c2cd5ff4a58f-at-[131.230.177.136]} } 9, 17 -- Date: Wed, 25 Jul 2007 15:16:24 -0500 } 9, 17 -- To: Microscopy-at-msa.microscopy.com } 9, 17 -- From: "John J. Bozzola" {bozzola-at-siu.edu} } 9, 17 -- Subject: Re: [Microscopy] AskAMicroscopist: } TEM of Arthrobacter GFB100 } 9, 17 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } 9, 17 -- X-Spam-Score: 0.00% } 9, 17 -- X-MASF: 0.00% } 9, 17 -- X-Whitelist: 0.00% } ==============================End of - } Headers============================== }
____________________________________________________________________________________ Pinpoint customers who are looking for what you sell. http://searchmarketing.yahoo.com/