Does anyone have a recommendation for software that will run an FFT on an image to find the REPEATING information (i.e. not remove the repeating noise)? We are collecting information on size and uniformity of arrays - not acutally using it for filtering.
The FFT in Image Pro is for filtering out the repeating information.
Thanks
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 6, 22 -- From edelmare-at-muohio.edu Wed Aug 1 08:34:01 2007 6, 22 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l71DY0dr019551 6, 22 -- for {microscopy-at-Microscopy.com} ; Wed, 1 Aug 2007 08:34:01 -0500 6, 22 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 6, 22 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l71DY0KJ021411 6, 22 -- for {microscopy-at-Microscopy.com} ; Wed, 1 Aug 2007 09:34:00 -0400 6, 22 -- Received: from [192.168.1.23] ([134.53.14.105]) 6, 22 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l71DY0tS021047 6, 22 -- for {microscopy-at-Microscopy.com} ; Wed, 1 Aug 2007 09:34:00 -0400 6, 22 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 6, 22 -- To: microscopy-at-Microscopy.com 6, 22 -- Date: Wed, 01 Aug 2007 09:34:00 -0400 6, 22 -- MIME-Version: 1.0 6, 22 -- Subject: FFT Software 6, 22 -- Message-ID: {46B05388.23485.AAB7611-at-edelmare.muohio.edu} 6, 22 -- Priority: normal 6, 22 -- X-mailer: Pegasus Mail for Windows (4.41) 6, 22 -- Content-type: text/plain; charset=US-ASCII 6, 22 -- Content-transfer-encoding: 7BIT 6, 22 -- Content-description: Mail message body 6, 22 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 ==============================End of - Headers==============================
You could just use imageJ, here is the link : http://rsb.info.nih.gov/ij/
Rachid
edelmare-at-muohio.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Does anyone have a recommendation for software that will run an FFT } on an image to find the REPEATING information (i.e. not remove the } repeating noise)? We are collecting information on size and } uniformity of arrays - not acutally using it for filtering. } } The FFT in Image Pro is for filtering out the repeating information. } } Thanks } -- Rachid SOUGRAT Cell Biology and Metabolism Branch NICHD, NIH Bldg. 18T, Rm. 101 18 Library Drive Bethesda, MD 20892-5430 USA
We have an old Link (Oxford) eXL computer, monitor, keyboard and mouse for giveaway. It is a parts only deal as we believe the CPU is dead. No detector. Must come and get it or arrange for packing and shipping yourself. Thanks
Mike ****************************************************************** Michael L. Boucher Sr. mboucher-at-tc.umn.edu Lab Manager Rm 18 Office Ph 612-624-6590 I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594 MiNTeC node of the National Nanotechnology Infrastructure Network
University of MN Fax 612-625-5368 12 Shepherd Labs 100 Union Street S.E. Minneapolis, MN 55455 http://www.charfac.umn.edu ********************************************************************
==============================Original Headers============================== 4, 22 -- From mboucher-at-umn.edu Wed Aug 1 09:30:20 2007 4, 22 -- Received: from mta-m2.tc.umn.edu (mta-m2.tc.umn.edu [134.84.119.106]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l71EUKdJ011875 4, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 1 Aug 2007 09:30:20 -0500 4, 22 -- Received: from mike (Mike.charfac.umn.edu [160.94.16.142]) 4, 22 -- by mta-m2.tc.umn.edu (UMN smtpd) with ESMTP 4, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 1 Aug 2007 09:30:20 -0500 (CDT) 4, 22 -- X-Umn-Remote-Mta: [N] Mike.charfac.umn.edu [160.94.16.142] #+LO+TS+AU+HN 4, 22 -- Reply-To: {mboucher-at-umn.edu} 4, 22 -- From: "Michael L. Boucher" {mboucher-at-umn.edu} 4, 22 -- To: {Microscopy-at-Microscopy.Com} 4, 22 -- Subject: EDS computer for giveaway 4, 22 -- Date: Wed, 1 Aug 2007 09:34:08 -0500 4, 22 -- Organization: University of MN 4, 22 -- Message-ID: {009d01c7d449$0599b8d0$8e105ea0-at-charfac.umn.edu} 4, 22 -- MIME-Version: 1.0 4, 22 -- Content-Type: text/plain; 4, 22 -- charset="US-ASCII" 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- X-Mailer: Microsoft Office Outlook 11 4, 22 -- thread-index: AcfUSQU8LMjXccL0SJywa89XyN/EYg== 4, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 ==============================End of - Headers==============================
If you have access to our iTEM software, you can set the filters to either "opaque" (filtering out the periodic structures), or "transparent", which filters out everything BUT the periodic information relating to that specific filter.
Have you checked if you can set this attribute in Image Pro?
Michael Bode, Ph.D.
General Manager
OLYMPUS SOFT IMAGING SOLUTIONS 12596 West Bayaud Ave #300 Lakewood, CO 80228 USA Tel.: +1 (303) 234-9270 Fax.: +1 (303) 234-9271 E-mail: Mike.Bode-at-olympus-sis.com www.olympus-sis.com
-----Original Message----- X-from: sougratr-at-mail.nih.gov [mailto:sougratr-at-mail.nih.gov] Sent: Wednesday, August 01, 2007 07:56 To: Mike Bode
You could just use imageJ, here is the link : http://rsb.info.nih.gov/ij/
Rachid
edelmare-at-muohio.edu wrote: } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Does anyone have a recommendation for software that will run an FFT on
} an image to find the REPEATING information (i.e. not remove the } repeating noise)? We are collecting information on size and } uniformity of arrays - not acutally using it for filtering. } } The FFT in Image Pro is for filtering out the repeating information. } } Thanks } -- Rachid SOUGRAT Cell Biology and Metabolism Branch NICHD, NIH Bldg. 18T, Rm. 101 18 Library Drive Bethesda, MD 20892-5430 USA
You can use Fovea Pro or Image Processing Toolkit, both from John and Chris Russ. Go to their website, http://www.reindeergraphics.com/. It will work in Photoshop and I think Image. On the disc that comes with the software is a complete tutorial on image processing and stereology. It's a good plug-in addition to Photoshop.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu] Sent: Wednesday, August 01, 2007 6:41 AM To: Walck-at-SouthBayTech.com
Does anyone have a recommendation for software that will run an FFT on an image to find the REPEATING information (i.e. not remove the repeating noise)? We are collecting information on size and uniformity of arrays - not acutally using it for filtering.
The FFT in Image Pro is for filtering out the repeating information.
Thanks
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 6, 22 -- From edelmare-at-muohio.edu Wed Aug 1 08:34:01 2007 6, 22 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l71DY0dr019551 6, 22 -- for {microscopy-at-Microscopy.com} ; Wed, 1 Aug 2007 08:34:01 -0500 6, 22 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 6, 22 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l71DY0KJ021411 6, 22 -- for {microscopy-at-Microscopy.com} ; Wed, 1 Aug 2007 09:34:00 -0400 6, 22 -- Received: from [192.168.1.23] ([134.53.14.105]) 6, 22 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l71DY0tS021047 6, 22 -- for {microscopy-at-Microscopy.com} ; Wed, 1 Aug 2007 09:34:00 -0400 6, 22 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 6, 22 -- To: microscopy-at-Microscopy.com 6, 22 -- Date: Wed, 01 Aug 2007 09:34:00 -0400 6, 22 -- MIME-Version: 1.0 6, 22 -- Subject: FFT Software 6, 22 -- Message-ID: {46B05388.23485.AAB7611-at-edelmare.muohio.edu} 6, 22 -- Priority: normal 6, 22 -- X-mailer: Pegasus Mail for Windows (4.41) 6, 22 -- Content-type: text/plain; charset=US-ASCII 6, 22 -- Content-transfer-encoding: 7BIT 6, 22 -- Content-description: Mail message body 6, 22 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 22 -- From walck-at-southbaytech.com Wed Aug 1 11:37:56 2007 15, 22 -- Received: from flpi101.prodigy.net (flpi101.sbcis.sbc.com [207.115.20.70]) 15, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l71Gbt2A008952 15, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Aug 2007 11:37:56 -0500 15, 22 -- X-ORBL: [64.169.217.123] 15, 22 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) 15, 22 -- by flpi101.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id l71Gbqws010160; 15, 22 -- Wed, 1 Aug 2007 09:37:52 -0700 15, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} 15, 22 -- To: {edelmare-at-muohio.edu} 15, 22 -- Cc: {Microscopy-at-microscopy.com} 15, 22 -- Subject: RE: [Microscopy] FFT Software 15, 22 -- Date: Wed, 1 Aug 2007 09:39:01 -0700 15, 22 -- Message-ID: {00bc01c7d45a$77c8ec30$7801a8c0-at-dynamicbl8uno3} 15, 22 -- MIME-Version: 1.0 15, 22 -- Content-Type: text/plain; 15, 22 -- charset="US-ASCII" 15, 22 -- Content-Transfer-Encoding: 7bit 15, 22 -- X-Mailer: Microsoft Office Outlook 11 15, 22 -- Thread-Index: AcfUQaKkWjbwk7MRQhKYi/r2n3GQ3AAGBFng 15, 22 -- In-Reply-To: {200708011341.l71DfFjG025229-at-ns.microscopy.com} 15, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 ==============================End of - Headers==============================
I am looking for a lab offering electropolishing services. My sample is alloy. I use ion milling, but I would like to see how electropolishing better woks for my sample. If you have some recommendation, please advise.
Thank you, Hiromi Konishi, Ph.D. UW-Madison
==============================Original Headers============================== 3, 28 -- From hikonishi3-at-gmail.com Wed Aug 1 12:24:15 2007 3, 28 -- Received: from nz-out-0506.google.com (nz-out-0506.google.com [64.233.162.238]) 3, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l71HOEaJ022423 3, 28 -- for {Microscopy-at-Microscopy.Com} ; Wed, 1 Aug 2007 12:24:15 -0500 3, 28 -- Received: by nz-out-0506.google.com with SMTP id o37so117595nzf 3, 28 -- for {Microscopy-at-Microscopy.Com} ; Wed, 01 Aug 2007 10:24:14 -0700 (PDT) 3, 28 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 3, 28 -- d=gmail.com; s=beta; 3, 28 -- h=domainkey-signature:received:received:message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 3, 28 -- b=EIlAFBSxi4TkhCMLRx/dK+OGKBT52vK6sowNkY9I/rzFdT1Rxha+al5NsQoy383o7FyQ3osfAnsGA+8M18qTJ6a8A3gio7zdqqvydbJGj+t7UGzHrMx6t2zB+sQiErmcs1bC4RVwy2/mBW57SWAlnDdK7SjzfpN6fklurNC96Bg= 3, 28 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 28 -- d=gmail.com; s=beta; 3, 28 -- h=received:message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 3, 28 -- b=gsjw4vFgR1YmNJjZZMNm77+1rjWyiP2/5FeoJg4we00YQA5R4Q/mwyjF+R17y+cf0EuxHT23Ac5hus9rXgGd+T6up6jrmJnRIkyeokx8Azm9WpsC5nHRzxhoMBIol/YgbYlr00H6VGL3paSxuJQshp0VYDA1/8WeSP8/sjiM14k= 3, 28 -- Received: by 10.143.157.10 with SMTP id j10mr43175wfo.1185989054186; 3, 28 -- Wed, 01 Aug 2007 10:24:14 -0700 (PDT) 3, 28 -- Received: by 10.143.16.11 with HTTP; Wed, 1 Aug 2007 10:24:14 -0700 (PDT) 3, 28 -- Message-ID: {456d33d00708011024k3b1efadkb0620a27c2c8d616-at-mail.gmail.com} 3, 28 -- Date: Wed, 1 Aug 2007 12:24:14 -0500 3, 28 -- From: "Hiromi Konishi" {hkonishi-at-wisc.edu} 3, 28 -- Sender: hikonishi3-at-gmail.com 3, 28 -- To: Microscopy-at-Microscopy.Com 3, 28 -- Subject: electropolishing service 3, 28 -- MIME-Version: 1.0 3, 28 -- Content-Type: text/plain; charset=ISO-8859-1 3, 28 -- Content-Transfer-Encoding: 7bit 3, 28 -- Content-Disposition: inline 3, 28 -- X-Google-Sender-Auth: 354f3c8c564d6cf2 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both susan.vanhorn-at-sunysb.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: susan.vanhorn-at-sunysb.edu Name: Susan C. Van Horn
Title-Subject: [Filtered] postembed with LR WHite
Question: I am doing postembed immuno on Ni formvar coated slot grids with sections embedded in LR White.....they seem to be falling off the grid before i get to counter stain them.....any reason why and how to avoid them coming off??? thanks, sue
I have lot of thermal papers rolls (Sony, TYPE IV, Enhanced, UPP-110HA, 110mmx18mm. I got digital image capturing system on my JEOL 5800 and now have no use of these. If you are interested, please contact me directly. Dont want to throw so many rolls away.
Javaid
==============================Original Headers============================== 6, 15 -- From javaidqazi-at-kemet.com Wed Aug 1 12:45:42 2007 6, 15 -- Received: from mail.kemet.com (mail1.kemet.com [204.128.147.15]) 6, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l71Hjdsm014118 6, 15 -- for {Microscopy-at-Microscopy.Com} ; Wed, 1 Aug 2007 12:45:41 -0500 6, 15 -- To: Microscopy-at-Microscopy.Com 6, 15 -- Subject: Free thermal papers to give away 6, 15 -- MIME-Version: 1.0 6, 15 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 6, 15 -- Message-ID: {OFEA19ACA7.C37A381D-ON8525732A.00612264-8525732A.00618C29-at-kemet.com} 6, 15 -- From: Javaid Qazi/QAE/HQ/KEMET/US {javaidqazi-at-kemet.com} 6, 15 -- Date: Wed, 1 Aug 2007 13:44:39 -0400 6, 15 -- X-MIMETrack: Serialize by Router on HQSMTPx1N/SERVERS/KEMET/US(Release 7.0.2FP2|May 14, 2007) at 6, 15 -- 08/01/2007 01:45:41 PM, 6, 15 -- Serialize complete at 08/01/2007 01:45:41 PM 6, 15 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
Job Description Test physical and chemical characteristics of new active pharmaceutical ingredients and drug products. Develop and validate analytical test procedures. Perform test method transfers. Knowledge of forensic microscopy techniques. Work in a cGMP laboratory. Strong emphasis on microscopy, spectroscopy and data handling. Good written and verbal skill.
Skills Must possess solid microscopy and microanalysis skills including polarized light microscopy, scanning electron microscopy, energy dispersive x-ray spectroscopy, and Fourier transform infrared spectroscopy in addition to basic analytical laboratory skills. Knowledge of and experience in drug development and working in accordance with GLP and GMP requirements is required. Additional skills in electron microscopy, energy dispersive x-ray spectroscopy and vibrational spectroscopy is desired. Excellent written and verbal communication is required. Please note: This position may be filled at a grade 13 or grade 15, depending on experience.
Education: BS with a minimum of 5 years directly related experience. Preferred Education. MS, chemistry is preferred.
Qualified applicants should send resume/CV to joe.neilly-at-abbott.com
==============================Original Headers============================== 5, 19 -- From joe.p.neilly-at-abbott.com Wed Aug 1 15:31:40 2007 5, 19 -- Received: from abtmx2.abbott.com (abtmx2.abbott.com [130.36.44.92]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l71KVeBb022280 5, 19 -- for {microscopy-at-microscopy.com} ; Wed, 1 Aug 2007 15:31:40 -0500 5, 19 -- Received: from abtapn561.northamerica.intra.abbott.com (abtapn561.northamerica.intra.abbott.com [10.236.188.103]) 5, 19 -- by abtmx2.abbott.com (Switch-3.1.8/Switch-3.1.7) with ESMTP id l71KVdab017093 5, 19 -- for {microscopy-at-microscopy.com} ; Wed, 1 Aug 2007 15:31:39 -0500 (CDT) 5, 19 -- To: microscopy-at-microscopy.com 5, 19 -- Cc: Pilar A Aquino {pilar.aquino-at-abbott.com} 5, 19 -- Subject: Analytical Chemist/Microscopist Position at Abbott Laboratories 5, 19 -- MIME-Version: 1.0 5, 19 -- X-Mailer: Lotus Notes Release 6.5.4 HF972 April 26, 2006 5, 19 -- Message-ID: {OFA95A2C17.80848DB2-ON8625732A.006F893A-8625732A.0070C254-at-abbott.com} 5, 19 -- From: Joseph P Neilly {joe.p.neilly-at-abbott.com} 5, 19 -- Date: Wed, 1 Aug 2007 15:30:48 -0500 5, 19 -- X-MIMETrack: Serialize by Router on ABTAPN561/ESVR/ABBOTT(Release 6.5.4FP2|September 12, 2005) at 5, 19 -- 08/01/2007 15:31:28, 5, 19 -- Serialize complete at 08/01/2007 15:31:28 5, 19 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fulton.2-at-osu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
We need to produce SEM and TEM images of Campylobacter flagella. Just wondering if anyone has experience with this and has a favorite protocol they might share with us. We have produced good images using uranyl acetate as a negative stain, but we are unsure of the proper buffer, stains, etc., for SEM and TEM that would give us good images of the cells with unbroken flagella. Thanks in advance for your time and trouble.
Dave, Working with a number of different bacteria I have found that for negative staining, fixation prior to staining gives better morphology, something like 2%PF + 2% GA in 0.1 M cacodylate (on the grid after adsorption) for 5-10 min. I also like to use fresh carbon coated grids, no parlodion. This will ensure that you see the flagella and pili with the thin carbon substrate. I have had success with both UA and PTA, remember though no phosphate when using UA to avoid precipitants. As for SEM it is difficult to avoid breakage of thin structures like membrane attachments and flagella, caused by dehydration. Perhaps a non-flexible substrate like silica chips or coverslips would be better than say formvar coated grids. Also you can try a chemical alternative to critical point drying like HMDS. Good Luck.
} Michael Delannoy } Dept of Cell Biology } Johns Hopkins School of Medicine } 725 N.Wolfe St. Physiology Bldg G-04 } Baltimore, Md 21205
----- Original Message ----- X-from: fulton.2-at-osu.edu
Position Description Academic Professional Associate in Physical, Material, and Geosciences
The Center for Ultrastructural Research (CUR) is seeking a scientist in materials characterization at the Ph.D. level. This individual will function at the professional/faculty level, collaborating with physical sciences users in experimental design, implementation, and analysis.
The individual to be hired will be expected to have accomplished original research, as witnessed by a publication record in materials sciences and/or related areas. He or she will be responsible for training and supervising users in TEM, SEM, electron diffraction, X-ray emission analysis, and X-ray microtomography. The individual will work to deliver pedagogic instruction in course offerings such as Electron Microscopy, Cellular Biology (i.e., Techniques in Modern Microscopy), and Clay Mineralogy.
The individual will assist physical sciences users of the CUR and share responsibility for the maintenance of instruments including: two TEM’s, two SEM’s, a confocal fluorescence microscope equipped for multiphoton microscopy with a femtosecond pulsed IR laser, a micro-computed tomography apparatus, and a deconvolution microscopy-capable inverted light microscope.
The individual to be hired will have the opportunity to conduct and publish independent research in his or her area of expertise and to submit grants both to support their own research and to augment the instrumentation at the CUR. Entry level salary will be around $47,000, with an increase commensurate with demonstrated experience. Inquiries and application should be directed to Dr. Paul A. Schroeder, Center for Ultrastructural Research, 151 Barrow Hall, University of Georgia, Athens, GA 30602 USA. (706) 542-4080 mailto:caur-at-uga.edu. Please include a curriculum vitae, statement of interest, and contact information for at least three references. Applications received before Aug. 24, 2007 will receive full consideration. The University of Georgia is an Equal Opportunity/Affirmative Action Institution. John P. Shields Center for Ultrastructural Research 151 Barrow Hall University of Georgia Athens, GA 30602
706-542-4080
==============================Original Headers============================== 7, 22 -- From jpshield-at-uga.edu Fri Aug 3 09:30:33 2007 7, 22 -- Received: from puntd4.cc.uga.edu (puntd4.cc.uga.edu [128.192.1.105]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l73EUX27002610 7, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 3 Aug 2007 09:30:33 -0500 7, 22 -- Received: from punts4.cc.uga.edu (punts4.cc.uga.edu [128.192.1.115]) 7, 22 -- by puntd4.cc.uga.edu (MOS 3.8.4-GA) 7, 22 -- with ESMTP id FWW12465; 7, 22 -- Fri, 3 Aug 2007 10:30:32 -0400 (EDT) 7, 22 -- Received: (from punts4.cc.uga.edu [128.192.63.198]) 7, 22 -- by punts4.cc.uga.edu (MOS 3.8.4-GA) 7, 22 -- with HTTPS/1.1 id FLZ05615 (AUTH jpshield-at-uga.edu); 7, 22 -- Fri, 3 Aug 2007 10:30:32 -0400 (EDT) 7, 22 -- From: John Shields {jpshield-at-uga.edu} 7, 22 -- Subject: mat sci position at UGA 7, 22 -- To: msa listserve {Microscopy-at-MSA.Microscopy.Com} 7, 22 -- X-Mailer: Mirapoint Webmail Direct 3.8.4-GA 7, 22 -- MIME-Version: 1.0 7, 22 -- Content-Type: text/plain; charset=UTF-8 7, 22 -- Message-Id: {20070803103032.FLZ05615-at-punts4.cc.uga.edu} 7, 22 -- Date: Fri, 3 Aug 2007 10:30:32 -0400 (EDT) 7, 22 -- Content-Transfer-Encoding: 8bit 7, 22 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id l73EUX27002610 ==============================End of - Headers==============================
Does anyone have experience freezing 4489 TEM film for long-term storage? I have stored many types of film at -20C with no problems, but haven't tried it with 4489. Can this cause condensation or other problems?
Ralph Common Michigan State University
==============================Original Headers============================== 4, 24 -- From rcommon-at-msu.edu Fri Aug 3 10:46:40 2007 4, 24 -- Received: from sys24.mail.msu.edu (sys24.mail.msu.edu [35.9.75.124]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l73Fke5W016458 4, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Aug 2007 10:46:40 -0500 4, 24 -- Received: from [35.9.122.125] (helo=emlab) 4, 24 -- by sys24.mail.msu.edu with esmtpsa (Exim 4.63 #1) 4, 24 -- (TLSv1:RC4-MD5:128) 4, 24 -- id 1IGzMN-0001p6-Lo 4, 24 -- for Microscopy-at-microscopy.com; Fri, 03 Aug 2007 11:46:39 -0400 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: Freezing 4489 Film 4, 24 -- Date: Fri, 3 Aug 2007 11:48:08 -0400 4, 24 -- Message-ID: {000f01c7d5e5$b0c758f0$7d7a0923-at-msu.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="iso-8859-1" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 (Normal) 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 4, 24 -- Importance: Normal 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1896 4, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
I am posting this on behalf of a colleague. This is a wafer fab in-line SEM I believe. Please send any responses to Ron or Donna. =========================================================================================
We have received the following request concerning the need for field service support for a "down" AMAT Opal 7830 SEM tool. I was wondering if any of you were aware of a field service engineer, or field service team that could provide repair on this tool. Please contact Donna Pistotti at dpistot1-at-irf.com if you have a solution or recommendation for her and cc me (ron.kee-at-avagotech.com) on your response so I can know if there is solution forthcoming, sooner than AMAT can respond on September 10...
Regards, Ron Kee Analysis Lab R&D manager Avago Technologies Ft. Collins, CO (970) 288-9389
-----Original Message----- X-from: Donna Pistotti [mailto:DPISTOT1-at-irf.com] Sent: Thursday, August 02, 2007 8:48 AM To: LT-at-waferfabs.org Cc: Sharon Noyes
We have had no problems feezing 4489 film at our school for long periods of time. Sometimes for several years. There has never been any problem with condensation.
Cathy Davis San Joaquin Delta College EM Lab
==============================Original Headers============================== 3, 22 -- From cdavis-at-deltacollege.edu Fri Aug 3 10:59:35 2007 3, 22 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236]) 3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l73FxYXG006524 3, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Aug 2007 10:59:35 -0500 3, 22 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 3, 22 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 3, 22 -- with ESMTP id 33948728 for Microscopy-at-microscopy.com; Fri, 03 Aug 2007 08:59:33 -0700 3, 22 -- Received: from deltacollege.edu ([172.20.2.118]) by 3, 22 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 3, 22 -- ESMTP id JM7G4E00.3TW for {Microscopy-at-microscopy.com} ; Fri, 3 3, 22 -- Aug 2007 08:53:02 -0700 3, 22 -- Message-ID: {46B350E5.B69546BB-at-deltacollege.edu} 3, 22 -- Date: Fri, 03 Aug 2007 08:59:31 -0700 3, 22 -- From: Cathy Davis {cdavis-at-deltacollege.edu} 3, 22 -- Organization: San Joaquin Delta College 3, 22 -- X-Mailer: Mozilla 4.77C-CCK-MCD {C-UDP; EBM-APPLE} (Macintosh; U; PPC) 3, 22 -- X-Accept-Language: en 3, 22 -- MIME-Version: 1.0 3, 22 -- To: Microscopy-at-microscopy.com 3, 22 -- Subject: 4489 Film 3, 22 -- Content-Type: text/plain; charset=us-ascii; x-mac-type="54455854"; x-mac-creator="4D4F5353" 3, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The votes are in and here's the partial summary of what people use to calibrate their TEM: My informal survey is based on 24 responses: 63% (14 people) use a grating replica, 68% (15 people) use crystal spacing.
26% of the respondents indicated they use a commercial product called Mag*I*Cal.
For more details and a little verbiage visit Microscopy Society of Northeastern Ohio Blog at: http://www.msneo.org/2007/08/scales-and-distance.html (my face will be really red, if this link doesn’t work!) You can also find what I think is the most unique TEM calibration I've ever read.
Have a great week-end
Frank
==============================Original Headers============================== 6, 19 -- From frank.karl-at-degussa.com Fri Aug 3 11:29:12 2007 6, 19 -- Received: from framailout1.rz.itson.com (mailout2.degussa.com [149.216.91.173]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l73GTBUF020218 6, 19 -- for {microscopy-at-msa.microscopy.com} ; Fri, 3 Aug 2007 11:29:11 -0500 6, 19 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [10.88.88.98]) 6, 19 -- by framailout1.rz.itson.com (8.13.4/8.13.4/Debian-3sarge3) with ESMTP id l73GT9Qt017516 6, 19 -- for {microscopy-at-msa.microscopy.com} ; Fri, 3 Aug 2007 18:29:09 +0200 6, 19 -- Subject: TEM Calibration details (sort of) 6, 19 -- To: microscopy-at-msa.microscopy.com 6, 19 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 6, 19 -- Message-ID: {OFB1E57B4B.EBCA430C-ON8625732C.0059B4FD-8525732C.005A8FE6-at-degussa.com} 6, 19 -- From: frank.karl-at-degussa.com 6, 19 -- Date: Fri, 3 Aug 2007 12:29:05 -0400 6, 19 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 6, 19 -- 08/03/2007 11:29:10 AM 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-type: text/plain; charset=UTF-8 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id l73GTBUF020218 ==============================End of - Headers==============================
Note that deadline is Nov. 1, 2007 not Aug. 24 Thanks
Position Description Academic Professional Associate in Physical, Material, and Geosciences
The Center for Ultrastructural Research (CUR) is seeking a scientist in materials characterization at the Ph.D. level. This individual will function at the professional/faculty level, collaborating with physical sciences users in experimental design, implementation, and analysis.
The individual to be hired will be expected to have accomplished original research, as witnessed by a publication record in materials sciences and/or related areas. He or she will be responsible for training and supervising users in TEM, SEM, electron diffraction, X-ray emission analysis, and X-ray microtomography. The individual will work to deliver pedagogic instruction in course offerings such as Electron Microscopy, Cellular Biology (i.e., Techniques in Modern Microscopy), and Clay Mineralogy.
The individual will assist physical sciences users of the CUR and share responsibility for the maintenance of instruments including: two TEM’s, two SEM’s, a confocal fluorescence microscope equipped for multiphoton microscopy with a femtosecond pulsed IR laser, a micro-computed tomography apparatus, and a deconvolution microscopy-capable inverted light microscope.
The individual to be hired will have the opportunity to conduct and publish independent research in his or her area of expertise and to submit grants both to support their own research and to augment the instrumentation at the CUR. Entry level salary will be around $47,000, with an increase commensurate with demonstrated experience. Inquiries and application should be directed to Dr. Paul A. Schroeder, Center for Ultrastructural Research, 151 Barrow Hall, University of Georgia, Athens, GA 30602 USA. (706) 542-4080 mailto:caur-at-uga.edu. Please include a curriculum vitae, statement of interest, and contact information for at least three references. Applications received before Nov. 1, 2007 will receive full consideration. The University of Georgia is an Equal Opportunity/Affirmative Action Institution. John P. Shields Center for Ultrastructural Research 151 Barrow Hall University of Georgia Athens, GA 30602
706-542-4080
==============================Original Headers============================== 6, 22 -- From jpshield-at-uga.edu Fri Aug 3 13:28:13 2007 6, 22 -- Received: from puntd2.cc.uga.edu (puntd2.cc.uga.edu [128.192.1.121]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l73ISBF5002715 6, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 3 Aug 2007 13:28:13 -0500 6, 22 -- Received: from punts4.cc.uga.edu (punts4.cc.uga.edu [128.192.1.115]) 6, 22 -- by puntd2.cc.uga.edu (MOS 3.8.4-GA) 6, 22 -- with ESMTP id FWL11103; 6, 22 -- Fri, 3 Aug 2007 14:28:10 -0400 (EDT) 6, 22 -- Received: (from punts4.cc.uga.edu [128.192.63.198]) 6, 22 -- by punts4.cc.uga.edu (MOS 3.8.4-GA) 6, 22 -- with HTTPS/1.1 id FMD05159 (AUTH jpshield-at-uga.edu); 6, 22 -- Fri, 3 Aug 2007 14:28:10 -0400 (EDT) 6, 22 -- From: John Shields {jpshield-at-uga.edu} 6, 22 -- Subject: date correction on UGA position 6, 22 -- To: msa listserve {Microscopy-at-MSA.Microscopy.Com} 6, 22 -- X-Mailer: Mirapoint Webmail Direct 3.8.4-GA 6, 22 -- MIME-Version: 1.0 6, 22 -- Content-Type: text/plain; charset=UTF-8 6, 22 -- Message-Id: {20070803142810.FMD05159-at-punts4.cc.uga.edu} 6, 22 -- Date: Fri, 3 Aug 2007 14:28:10 -0400 (EDT) 6, 22 -- Content-Transfer-Encoding: 8bit 6, 22 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id l73ISBF5002715 ==============================End of - Headers==============================
Hi Pat, I may have missed that note, our server somehow misplaces things. I checked on Mag*I*Cal and you are correct. NIST doesn't calibrate " fundamental constants of nature." Seems a shame.
Thanks for the note!
Enjoy the week-end!
Patricia Connelly {connellyps-at-nhlbi To: {frank.karl-at-degussa.com} .nih.gov} cc: Subject: Re: [Microscopy] TEM Calibration details (sort of) 08/03/2007 02:34 PM
Frank, I think that there was a correction by the microscopist who gave the message about the NIST traceability. If I remember correctly he wrote "now" and corrected it to "not".
Pat Connelly
==============================Original Headers============================== 16, 18 -- From frank.karl-at-degussa.com Fri Aug 3 13:59:48 2007 16, 18 -- Received: from framailout1.rz.itson.com (mailout2.degussa.com [149.216.91.173]) 16, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l73Ixlpr015201 16, 18 -- for {microscopy-at-msa.microscopy.com} ; Fri, 3 Aug 2007 13:59:48 -0500 16, 18 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [10.88.88.98]) 16, 18 -- by framailout1.rz.itson.com (8.13.4/8.13.4/Debian-3sarge3) with ESMTP id l73IxeTm014185; 16, 18 -- Fri, 3 Aug 2007 20:59:42 +0200 16, 18 -- In-Reply-To: {C2D8ED81.9E5%connellyps-at-nhlbi.nih.gov} 16, 18 -- Subject: Re: [Microscopy] TEM Calibration details (sort of) 16, 18 -- To: connellyps-at-nhlbi.nih.gov, microscopy-at-msa.microscopy.com 16, 18 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 16, 18 -- Message-ID: {OF15322B36.87C9C88E-ON8625732C.0067D509-8525732C.006857F0-at-degussa.com} 16, 18 -- From: frank.karl-at-degussa.com 16, 18 -- Date: Fri, 3 Aug 2007 14:59:36 -0400 16, 18 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 16, 18 -- 08/03/2007 01:59:45 PM 16, 18 -- MIME-Version: 1.0 16, 18 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Job Summary: Chromodynamics Inc. is seeking a Product/Sales Management candidate with a science background and knowledge of the microscope community, strong computer skills and at least one year of hands-on experience using a Confocal or similar advanced light microscope for its new hyperspectral imaging product line.
Location: Orlando, FL or Lakewood, NJ
Responsibilities:
- Customer visits and analysis of customer's imaging needs - Demonstration of products and onsite work with the customer - Sales support of existing customers - Identifying potential new customers via web searches, literature searches, marketing campaigns, trade shows, and customer referrals - Introduction of Chromodynamics products and services to potential customers via in person visits, email and phone - Understanding potential customers needs related to products offered by Chromodynamics Inc. - Organizing workshops and demonstrations for regional sales representatives, distributors and VARs (value-added resellers) - Monitor daily/monthly orders and provide information in regards to account details - Expand sales channels to increase market penetration in designated product areas and markets. - Attain maximum sales and appropriate product mix. - Uncover new sales opportunities through cold calling, networking and other proven marketing strategies - Relay leads to dealer personnel, follow up and monitor outcome - Watch for and identify new markets - Set and achieve established sales goals - Develop thorough knowledge of all frequently encountered applications and their appropriate solutions for which related products are required - Report significant changes or trends in area sales. - Provide feedback on product quality, needs, competition, business trends and unique product applications to management team - Complete required reports - Maintain files and records of customer names, orders and locations - Develop strategic marketing plan and coordinate the sales activities in conjunction with Engineering and Operations to achieve stated goals
Reporting directly to the Vice-President of Marketing and Sales, this key contributor will work with our potential and existing clients, through direct and indirect channels, to meet their life science imaging needs with Chromodynamics Inc. leading solutions and expertise.
As the customer's point of contact, they must be able to provide applications assistance, product support and sales of Chromodynamics Inc. products and services to technical buyers. Must have experience responding to tenders, RFPs, RFQs, generating contracts, bid proposals and supporting the negotiation and closing sales with our distributors and representatives. They will follow up on quotations, drive processing of orders and maintain adequate records to document price quotations, decisions, progress and results of activity. They will use available resources such as sales leads, literature, samples and demos, telephones, Internet and email, computers and support staff and services to produce the most effective results and exercise strong administrative, organizational and communication skills.
Minimum Desired Qualifications:
A Bachelors degree in the sciences (i.e. biology, chemistry, physics or equivalent) and a minimum of 4 years experience or a graduate degree or equivalent and a minimum of 2 years of applicable laboratory and sales experience required. Proficient use of personal computers: Windows 95/98, NT, XP, MS Word, Excel, PowerPoint, Internet Explorer, Outlook, etc. Must demonstrate the ability to simplify problems, articulate solutions, lead and delegate, have excellent communication skills and be a highly motivated team oriented self-starter. Travel required up to 25%.
Contact:
ChromoDynamics, Inc. 1195 Airport Road, #1 Lakewood, New Jersey 08701 Fax: 732.730.3547 Email: careers-at-olinet.com Web: http://www.chromodynamics.net/
==============================Original Headers============================== 12, 32 -- From afong-at-olinet.com Fri Aug 3 16:23:41 2007 12, 32 -- Received: from renoir.hmdnsgroup.com (renoir.hmdnsgroup.com [63.247.141.9]) 12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l73LNf09030587 12, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Aug 2007 16:23:41 -0500 12, 32 -- Received: from [216.189.208.196] (port=4520 helo=VPSales) 12, 32 -- by renoir.hmdnsgroup.com with esmtpa (Exim 4.66) 12, 32 -- (envelope-from {afong-at-olinet.com} ) 12, 32 -- id 1IH4cY-0000z4-8B 12, 32 -- for Microscopy-at-microscopy.com; Fri, 03 Aug 2007 17:23:42 -0400 12, 32 -- Reply-To: {afong-at-olinet.com} 12, 32 -- From: "Alex Fong" {afong-at-olinet.com} 12, 32 -- To: {Microscopy-at-microscopy.com} 12, 32 -- Date: Fri, 3 Aug 2007 17:23:25 -0400 12, 32 -- Organization: Optronic Laboratories 12, 32 -- Message-ID: {017901c7d614$94fc9660$bef5c320$-at-com} 12, 32 -- MIME-Version: 1.0 12, 32 -- Content-Type: text/plain; 12, 32 -- charset="us-ascii" 12, 32 -- Content-Transfer-Encoding: 7bit 12, 32 -- X-Mailer: Microsoft Office Outlook 12.0 12, 32 -- Thread-Index: AcfWFH3ABcOFF79EQICpcSQnBAy/tA== 12, 32 -- Content-Language: en-us 12, 32 -- X-HMDNSGroup-MailScanner-Information: Please contact the ISP for more information 12, 32 -- X-HMDNSGroup-MailScanner: Found to be clean 12, 32 -- X-HMDNSGroup-MailScanner-SpamCheck: 12, 32 -- X-HMDNSGroup-MailScanner-From: afong-at-olinet.com 12, 32 -- Subject: Job Posting: Product Marketing/Sales Manager 12, 32 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 12, 32 -- X-AntiAbuse: Primary Hostname - renoir.hmdnsgroup.com 12, 32 -- X-AntiAbuse: Original Domain - microscopy.com 12, 32 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 12, 32 -- X-AntiAbuse: Sender Address Domain - olinet.com ==============================End of - Headers==============================
LM and imaging folk--we have a first-generation zeiss axioskop that we've just set up with a scion digital camera and diagnostic instruments coupler for transmitted-light photomicrography. The illuminated field is hideously uneven, with a central hot-spot. The Scion doesn't do shading correction, so, aside from flat-field correction in ImageJ, has anyone else experienced/solved this problem? Will a diffuser in the beam path help? TIA Julian -- Julian P.S. Smith III Dept. of Biology Winthrop University 520 Cherry Rd. Rock Hill, SC 29733
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pmoeck-at-pdx.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: pmoeck-at-pdx.edu Name: Peter Moeck
Organization: Portland State University
Title-Subject: [Filtered] Postdoc up to 3 years, Crystallographic and spectroscopic analyses of ferromagnetic semiconducting nanoparticle aggregates
Question: Postdoc (USA, University of Davis, California and west coast based) for a collaboration between Portland State University, the University of California at Davis, the University of Washington at Seattle, and the Pacific NorthWest National Laboratory
Initially for one year at $35,500 plus the typical fringe benefits (health insurance, retirement benefits, etc. ... package that ammounts to about $17,500 annually) available from September 1st, 2007, onwards, extendable up to 3 years by mutual agreement.
A group of collaborators from Portland State University, the University of California at Davis, and the University of Washington at Seattle is seeking a postdoc for a project on Crystallographic and spectroscopic analyses of ferromagnetic semiconducting nanoparticle aggregates with Curie temperatures well above room temperature.
A background in materials physics, materials chemistry, crystallography, or materials science and engineering is required. Familiarity with Z-contrast (HAADF) imaging in scanning transmission electron microscopes and associated electron energy loss spectroscopy are essential. Skills in high resolution phase-contrast transmission electron microscopy, electron diffraction and crystallography, and crystallographic image processing will be appreciated.
The search will be open until the position has been filled. Applications (CV, list of referees, reasons for coming to the US for applicants from aboard, list of publications, etc.) should be sent to both
Prof. Peter Moeck Department of Physics Portland State University P.O. Box 751 Portland, Oregon 97207-0751 Tel.: 503 725 4227 Fax: 503 725 2815 e-mail: pmoeck-at-pdx.edu
and
Prof. Nigel D. Browning Department of Chemical Engineering and Materials Science University of California-Davis One Shields Ave Davis, CA 95616 Tel: 530-754-5358 (Davis) Fax: 530-752-9554 (Davis) e-mail: nbrowning-at-ucdavis.edu
We are bringing to your attention the workshop "Piezoresponse Force Microscopy: Instrumentation, Techniques, and Applications" to be held at Oak Ridge, TN on October 8-9, 2007, in conjunction with the CNMS User Meeting (http://cnms.ornl.gov/). The workshop focuses on electromechanical coupling in inorganic and macromolecular materials and biological systems and will feature invited tutorials on emergent phenomena in nanoscale ferroelectrics and ferroelectric surfaces, polarization-mediated surface phenomena in polar materials, and piezoelectricity and electrophysiology of biosystems. The central theme of the workshop - Piezoresponse Force Microscopy - will be discussed in a series of tutorials by S.V. Kalinin (ORNL) and A. Gruverman (UNL), that will introduce basic principles of PFM operation, relevant instrumental aspects, and recent advances in PFM imaging of switching ferroelectric films and nanostructures, biological materials and macromolecular systems, and PFM in liquid and ultra high vacuum environment. The tutorials will be complemented by a poster session.
The 2 day workshop will be followed by 3 days of experimental demonstrations of PFM, Switching Spectroscopy PFM, and band-excitation PFM by CNMS staff members and Asylum Research representatives, held in parallel with the CNMS user meeting (October 10-12). The participants are welcome to bring their own samples. The workshop abstract and outline are available on line at http://cnms.ornl.gov/workshops/CNMS_PFM_Workshop.pdf. The registration information will be available shortly at http://neutrons.ornl.gov/workshops/users2007/
The invited speakers include:
M. Gregg (U. Belfast), "Nanoscale Ferroelectrics: Where Size Really Does Matter"
S. Streiffer (Argonne National Lab.), "Phase transitions, domain structure & dynamics, and surface chemistry in ferroelectrics studied by x-rays"
Andrew M. Rappe (University of Pennsylvania), "First-principles and multiscale modeling of ferroelectrics"
Andrew Marino (LSU), "Electromechanics of biosystems"
Due to the space limit, please contact workshop organizers [sergei2-at-ornl.gov] by August 31 if you are interested in attending.
Yours
Sergei V. Kalinin and Arthur P. Baddorf
==============================Original Headers============================== 12, 24 -- From sergei2-at-ornl.gov Mon Aug 6 14:20:50 2007 12, 24 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119]) 12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l76JKoNM027019 12, 24 -- for {microscopy-at-microscopy.com} ; Mon, 6 Aug 2007 14:20:50 -0500 12, 24 -- Received: from emroute1.ornl.gov ([127.0.0.1]) 12, 24 -- by emroute1.ornl.gov (PMDF V6.3-x11 #31501) 12, 24 -- with ESMTP id {0JMD00F4P9QO7K-at-emroute1.ornl.gov} for 12, 24 -- microscopy-at-microscopy.com; Mon, 06 Aug 2007 15:20:49 -0400 (EDT) 12, 24 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov 12, 24 -- (PMDF V6.3-x11 #31501) id {0JMD00H019QOE0-at-emroute1.ornl.gov} for 12, 24 -- microscopy-at-microscopy.com; Mon, 06 Aug 2007 15:20:48 -0400 (EDT) 12, 24 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 12, 24 -- by emroute1.ornl.gov (PMDF V6.3-x11 #31501) 12, 24 -- with ESMTP id {0JMD00F6T9QO7X-at-emroute1.ornl.gov} for 12, 24 -- microscopy-at-microscopy.com; Mon, 06 Aug 2007 15:20:48 -0400 (EDT) 12, 24 -- Date: Mon, 06 Aug 2007 15:20:48 -0400 12, 24 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 12, 24 -- Subject: PFM Workshop - Oak Ridge, October 8,9 12, 24 -- To: microscopy-at-microscopy.com 12, 24 -- Message-id: {46B77490.3050408-at-ornl.gov} 12, 24 -- MIME-version: 1.0 12, 24 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 12, 24 -- Content-transfer-encoding: 7bit 12, 24 -- User-Agent: Thunderbird 1.5.0.12 (Windows/20070509) ==============================End of - Headers==============================
Please forgive me for using the list to try and solve a personal problem, but I cannot obtain an answer to my question by the official route, so I send this message in a bottle in the hope that some personality involved in the conference will read it and answer it.
I simply would like to know the subjects of the different workshops planned during the conference.
Regards,
Stephane
____________________________________________________________________________________ Shape Yahoo! in your own image. Join our Network Research Panel today! http://surveylink.yahoo.com/gmrs/yahoo_panel_invite.asp?a=7
==============================Original Headers============================== 9, 19 -- From nizets2-at-yahoo.com Tue Aug 7 07:29:52 2007 9, 19 -- Received: from web37409.mail.mud.yahoo.com (web37409.mail.mud.yahoo.com [209.191.91.141]) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l77CTqXv015144 9, 19 -- for {microscopy-at-microscopy.com} ; Tue, 7 Aug 2007 07:29:52 -0500 9, 19 -- Received: (qmail 43310 invoked by uid 60001); 7 Aug 2007 12:29:51 -0000 9, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 19 -- s=s1024; d=yahoo.com; 9, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 9, 19 -- b=be3Sg9hI6IrIf4loFcboyOP4t07S2TKZb9M5ZLx2euvSX87vpQHPNgeEqVJk9+MrZE3HpfLKMExxrgPBfFgMFXB4MK3jJjQ8YrrPLZRt2avCvTwqI0BscZaZSAFqwdGpR+fHPqIbNbKrt9Ndx7DpbWFXC/UO9gkyCqblPy8Y4VY=; 9, 19 -- X-YMail-OSG: 7cEyWPgVM1lpM8UnoqwF21mWMDXrBOVIyXIqpmtjaAh.UtJRNFrGG9ZRLmUR4pevtPI2WhCSzJbiUJCrJpA_N614MKHSu.uo3y8nO.1vS3o70FPwd0lfWU.sHMB8Lw-- 9, 19 -- Received: from [80.122.101.102] by web37409.mail.mud.yahoo.com via HTTP; Tue, 07 Aug 2007 05:29:51 PDT 9, 19 -- Date: Tue, 7 Aug 2007 05:29:51 -0700 (PDT) 9, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 9, 19 -- Subject: MC 2007 in Saarbrücken, Germany 9, 19 -- To: microscopy-at-microscopy.com 9, 19 -- MIME-Version: 1.0 9, 19 -- Content-Type: text/plain; charset=iso-8859-1 9, 19 -- Content-Transfer-Encoding: 8bit 9, 19 -- Message-ID: {320961.43014.qm-at-web37409.mail.mud.yahoo.com} ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both reznik-at-ict.uni-karlsruhe.de as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: reznik-at-ict.uni-karlsruhe.de Name: Boris
Title-Subject: [Filtered] SEM_S570_1
Question: I have an old Hitachi SEM, S-570 with a digital photocamera NikonD100 attached to the camera unit. At the observation CRT the SEM-images are sharp while at the CCD the images are not sharp at different camera positions. I checked the CCD camera as I took pictures directly from the observation CRT, and the pictures are sharp. I think that something is wrong with the justage or quality of the photo CRT. Does anybody know, how can I overcome my problem?
First thing to do, if you possibly can, is to check the quality of the photo CRT by shooting some conventional film (say Polaroid). This is fairly important since it will tell you if its a problem with the photo CRT.
Is this the first time setting up the 570 with a digital camera or was it working fine and then became unsharp?
We have a Hitachi S2460N to which I attached a Nikon D70S digital camera. It works fine but one must be very careful setting the camera focus on the CRT is critical. Is it possible that the camera is slipping out of focus (loose components)?
JB
} Question: I have an old Hitachi SEM, S-570 with a digital photocamera } NikonD100 attached to the camera unit. At the observation CRT the } SEM-images are sharp while at the CCD the images are not sharp at } different camera positions. I checked the CCD camera as I took } pictures directly from the observation CRT, and the pictures are } sharp. I think that something is wrong with the justage or quality of } the photo CRT. } Does anybody know, how can I overcome my problem? } } Boris } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Tue Aug 7 13:06:55 2007 } 6, 11 -- Received: from [65.213.163.180] (msdvpn8.msd.anl.gov } [130.202.238.72]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l77I6rfh001070 } 6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 7 Aug 2007 13:06:54 -0500 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p0624080bc2de6535d598-at-[65.213.163.180]} } 6, 11 -- Date: Tue, 7 Aug 2007 13:07:00 -0500 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: reznik-at-ict.uni-karlsruhe.de (by way of MicroscopyListserver) } 6, 11 -- Subject: viaWWW: SEM_S570_1 } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both David.Llewellyn-at-anu.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: David.Llewellyn-at-anu.edu.au Name: David Llewellyn
Question: Hi all, would anyone have a manual for the thickness coater complete with circuit diagrams, I need to carry out a fix on one of these devices, thanks, David.
I am looking for a double tilt holder for a JEOL 200CX TEM. OEM or Gatan is fine, I'm not picky.
If you know of any out there please let me know.
Thanks,
Tom
Thomas M. Murray Senior Research Support Specialist / Instructor College of Nanoscale Science & Engineering University at Albany-SUNY 251 Fuller Rd. Albany, NY 12203
I am having a too strong signal in EDX (in SEM). The dead time is usually between 70-80%. I wondered how I could reduce the signal without changing the HT. Changing the size of the beam is one way. Would it be wise to move the stage in the Z axis to be sub-optimal with respect to the EDX detector? Any other idea?
Stephane
____________________________________________________________________________________ Got a little couch potato? Check out fun summer activities for kids. http://search.yahoo.com/search?fr=oni_on_mail&p=summer+activities+for+kids&cs=bz
==============================Original Headers============================== 5, 19 -- From nizets2-at-yahoo.com Thu Aug 9 07:44:44 2007 5, 19 -- Received: from web37403.mail.mud.yahoo.com (web37403.mail.mud.yahoo.com [209.191.91.135]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l79CiixH016265 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 9 Aug 2007 07:44:44 -0500 5, 19 -- Received: (qmail 52807 invoked by uid 60001); 9 Aug 2007 12:44:43 -0000 5, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 19 -- s=s1024; d=yahoo.com; 5, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 5, 19 -- b=twSYQrDDLa/tW+xKPh1Yn+iU+6fCqgxYvW3XHdl/QAfyM4ORoQ0OfQIoZwsrcpXg43ky3xSKxLhM90woedzeZ0NHay1zcoR+gWOwula4qVNIOujW5oXuUwSUX4ouilINd5/HxZMSXDncJaclQRpQDmmbD3S8szeWAsk9qb3y/9Q=; 5, 19 -- X-YMail-OSG: CWUsP7oVM1mv2zg2qW3XpSd9xm15WwWQVNQ6WY4qsD3JjeXQYcsXcQSMaciLYDVW9jC0P21_gdeVXkj686T_ZB2pykfpEej16rLDeqGcPdUprejITs51nKFp8lDF6w-- 5, 19 -- Received: from [80.122.101.102] by web37403.mail.mud.yahoo.com via HTTP; Thu, 09 Aug 2007 05:44:43 PDT 5, 19 -- Date: Thu, 9 Aug 2007 05:44:43 -0700 (PDT) 5, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 19 -- Subject: question about EDX signal saturation 5, 19 -- To: microscopy-at-microscopy.com 5, 19 -- MIME-Version: 1.0 5, 19 -- Content-Type: text/plain; charset=iso-8859-1 5, 19 -- Content-Transfer-Encoding: 8bit 5, 19 -- Message-ID: {235686.52530.qm-at-web37403.mail.mud.yahoo.com} ==============================End of - Headers==============================
Dear Stephane, It is not a good idea to move the Z axis of the SEM stage for EDX, this will upset the geometry of the beam-detector system and affect quantitation. There are three possibilities: get an SDD detector, which is much more tolerant of high beam currents, use a higher condenser lens setting (smaller beam spot size) or a smaller final aperture, if your SEM has a movable final aperture. You could also move the EDX detector back along its track (retract it), which will change the solid angle but not the geometry. You may have to re-enter the geometry in the EDX setup if you do that. If you change the HT of the SEM, you may not detect all the elements. Good luck,
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: August 9, 2007 5:54 AM To: maryflet-at-interchange.ubc.ca
Hi all!
I am having a too strong signal in EDX (in SEM). The dead time is usually between 70-80%. I wondered how I could reduce the signal without changing the HT. Changing the size of the beam is one way. Would it be wise to move the stage in the Z axis to be sub-optimal with respect to the EDX detector? Any other idea?
Stephane
____________________________________________________________________________ ________ Got a little couch potato? Check out fun summer activities for kids. http://search.yahoo.com/search?fr=oni_on_mail&p=summer+activities+for+kids&c s=bz
==============================Original Headers============================== 5, 19 -- From nizets2-at-yahoo.com Thu Aug 9 07:44:44 2007 5, 19 -- Received: from web37403.mail.mud.yahoo.com (web37403.mail.mud.yahoo.com [209.191.91.135]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l79CiixH016265 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 9 Aug 2007 07:44:44 -0500 5, 19 -- Received: (qmail 52807 invoked by uid 60001); 9 Aug 2007 12:44:43 -0000 5, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 19 -- s=s1024; d=yahoo.com; 5, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Conten t-Transfer-Encoding:Message-ID; 5, 19 -- b=twSYQrDDLa/tW+xKPh1Yn+iU+6fCqgxYvW3XHdl/QAfyM4ORoQ0OfQIoZwsrcpXg43ky3xSKxL hM90woedzeZ0NHay1zcoR+gWOwula4qVNIOujW5oXuUwSUX4ouilINd5/HxZMSXDncJaclQRpQDm mbD3S8szeWAsk9qb3y/9Q=; 5, 19 -- X-YMail-OSG: CWUsP7oVM1mv2zg2qW3XpSd9xm15WwWQVNQ6WY4qsD3JjeXQYcsXcQSMaciLYDVW9jC0P21_gdeV Xkj686T_ZB2pykfpEej16rLDeqGcPdUprejITs51nKFp8lDF6w-- 5, 19 -- Received: from [80.122.101.102] by web37403.mail.mud.yahoo.com via HTTP; Thu, 09 Aug 2007 05:44:43 PDT 5, 19 -- Date: Thu, 9 Aug 2007 05:44:43 -0700 (PDT) 5, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 19 -- Subject: question about EDX signal saturation 5, 19 -- To: microscopy-at-microscopy.com 5, 19 -- MIME-Version: 1.0 5, 19 -- Content-Type: text/plain; charset=iso-8859-1 5, 19 -- Content-Transfer-Encoding: 8bit 5, 19 -- Message-ID: {235686.52530.qm-at-web37403.mail.mud.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 14, 35 -- From maryflet-at-interchange.ubc.ca Thu Aug 9 12:08:17 2007 14, 35 -- Received: from mr6.mail-relay.ubc.ca (mr6.mail-relay.ubc.ca [137.82.45.11]) 14, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l79H8HKk004918 14, 35 -- for {microscopy-at-microscopy.com} ; Thu, 9 Aug 2007 12:08:17 -0500 14, 35 -- Received: from mr6.mail-relay.ubc.ca (localhost [127.0.0.1]) 14, 35 -- by localhost (Postfix) with SMTP id 68B3B15A6B 14, 35 -- for {microscopy-at-microscopy.com} ; Thu, 9 Aug 2007 10:08:16 -0700 (PDT) 14, 35 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69]) 14, 35 -- by mr6.mail-relay.ubc.ca (Postfix) with ESMTP 14, 35 -- for {microscopy-at-microscopy.com} ; Thu, 9 Aug 2007 10:08:09 -0700 (PDT) 14, 35 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 14, 35 -- by smtp.interchange.ubc.ca 14, 35 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 14, 35 -- with ESMTPS id {0JMI007GNNLLGE-at-smtp.interchange.ubc.ca} for 14, 35 -- microscopy-at-microscopy.com; Thu, 09 Aug 2007 10:08:09 -0700 (PDT) 14, 35 -- Date: Thu, 09 Aug 2007 10:05:44 -0700 14, 35 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 14, 35 -- Subject: RE: [Microscopy] question about EDX signal saturation 14, 35 -- In-reply-to: {200708091254.l79Cs8OJ027428-at-ns.microscopy.com} 14, 35 -- To: nizets2-at-yahoo.com 14, 35 -- Cc: microscopy-at-microscopy.com 14, 35 -- Reply-to: maryflet-at-interchange.ubc.ca 14, 35 -- Message-id: {0JMI007GONLLGE-at-smtp.interchange.ubc.ca} 14, 35 -- Organization: Materials Eng. 14, 35 -- MIME-version: 1.0 14, 35 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 14, 35 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 14, 35 -- Content-type: text/plain; charset=us-ascii 14, 35 -- Content-transfer-encoding: 7bit 14, 35 -- Thread-index: AcfahGBTC0SEPyvmScWDAa4qjLe2OAAIhvEQ 14, 35 -- X-UBC-Scanned: Sophos PureMessage 5.3.2.304607, Antispam-Engine: 2.5.1.298604, Antispam-Data: 2007.8.9.94623 14, 35 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 14, 35 -- X-PerlMx-Spam: Probability=7%, Report=__C230066_P5 0, __CP_NAME_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __USER_AGENT_MS_GENERIC 0, __pbl.spamhaus.org_TIMEOUT , __sbl.spamhaus.org_TIMEOUT 14, 35 -- X-Spam-Level: 14, 35 -- X-Spam-Flag: No ==============================End of - Headers==============================
Please see the announcement for a free workshop on microtomy. Thanks.
Chunfei Li Portland State University
Agenda Unless specified, lecture and training will be conducted by Dave Roberts/Greg Becker from RMC Products
Sep. 13 (Thursday), 2007 8:30AM Introduction: Goals and schedule. Get acquainted with participants and their projects (Chunfei Li, PSU; Dave Roberts/Greg Becker, RMC) 9:00AM Short PowerPoint presentation on ultramicrotomy for materials and biological applications 10:00AM Ultramicrotomy of very hard specimens. Presentation and discussion (Unity Semiconductor, Phil Swab) 11:00AM Practical demonstration of room temperature ultramicrotomy 12:00AM Lunch Break 1:00PM Hands-on workshop for room temperature ultramicrotomy 3:00PM Glass knife making demonstration and discussion on ultramicrotomy knives 3:30PM Further hands-on room temperature ultramicrotomy 5:00PM Discussion of the days results
Sept. 14 (Friday), 2007 08:30 FIB specimen preparation of softmaterial and Tomography (FEI, Richard Gursky) 09:30 High pressure freezing (Shriner Hospital, Doug Keene) 10:30 Practical cryoultramicrotomy demonstration 11:00 Hands-on cryoultramicrotomy 12:00 Lunch 1:00 Further hands-on cryoultramicrotomy 4:30 Discussion of cryoultramicrotomy results. Ultramicrotomy problem solving. 5:00 Conclusion of workshop
Pre-registration Form Although drop-in attendants are welcome, pre-registration is strongly suggested for better coordination. Name: Affiliation: phone/e-mail: Do you intend to bring in a sample for demonstration/training? Yes/No Do you need parking? Yes/No Please E-mail back to mazzio-at-pdx.edu or mail to Portland State University, Department of Physics Attn: Katherine Mazzio/Chunfei Li P.O. Box 751, Portland, OR 97207-0751
==============================Original Headers============================== 18, 32 -- From chunfei-at-pdx.edu Thu Aug 9 12:25:07 2007 18, 32 -- Received: from njord.oit.pdx.edu (njord.oit.pdx.edu [131.252.120.57]) 18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l79HP7S4016822 18, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Aug 2007 12:25:07 -0500 18, 32 -- Received: from grerr.oit.pdx.edu (grerr.oit.pdx.edu [131.252.120.19]) 18, 32 -- by njord.oit.pdx.edu (8.13.3+/8.13.1) with ESMTP id l79HP5AH011527 18, 32 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 18, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Aug 2007 10:25:05 -0700 18, 32 -- Received: from grerr.oit.pdx.edu (localhost.localdomain [127.0.0.1]) 18, 32 -- by grerr.oit.pdx.edu (8.13.3+/8.13.1) with ESMTP id l79HP5Hf030000 18, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Aug 2007 10:25:05 -0700 18, 32 -- X-Authentication-Warning: grerr.oit.pdx.edu: Host localhost.localdomain [127.0.0.1] claimed to be grerr.oit.pdx.edu 18, 32 -- Received: (from sec-at-localhost) 18, 32 -- by grerr.oit.pdx.edu (8.13.3+/8.13.1) id l79HP5x4029999 18, 32 -- for Microscopy-at-microscopy.com; Thu, 9 Aug 2007 10:25:05 -0700 18, 32 -- Received: from host-124-220.dhcp.pdx.edu (host-124-220.dhcp.pdx.edu 18, 32 -- [131.252.124.220]) by webmail.pdx.edu (Horde MIME library) with HTTP; Thu, 18, 32 -- 09 Aug 2007 10:25:05 -0700 18, 32 -- Message-ID: {20070809102505.q77od39l2skkwcws-at-webmail.pdx.edu} 18, 32 -- Date: Thu, 09 Aug 2007 10:25:05 -0700 18, 32 -- From: chunfei-at-pdx.edu 18, 32 -- To: Microscopy-at-microscopy.com 18, 32 -- Subject: microtomy workshop at Portland State University, Sept. 13-14 18, 32 -- MIME-Version: 1.0 18, 32 -- Content-Type: text/plain; 18, 32 -- charset=ISO-8859-1; 18, 32 -- DelSp="Yes"; 18, 32 -- format="flowed" 18, 32 -- Content-Disposition: inline 18, 32 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.3) 18, 32 -- Content-Transfer-Encoding: 8bit 18, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l79HP7S4016822 ==============================End of - Headers==============================
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7ABAFVS017519 for {MicroscopyListserverArchive-at-microscopy.com} ; Fri, 10 Aug 2007 06:10:15 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id l7ABAF02017517; Fri, 10 Aug 2007 06:10:15 -0500
Thank you Mary (and Austin too and all the others) for your valuable opinion. As someone pointed out, when I decrease the beam size my signal immediately disappears under the noise in BSE(i am at step 2 on a scale of 20). As for the "time constant", I think on my SEM (it is a Tescan) it is called "process time", I will check this parameter but I don't think it will change much, I must keep it quite high otherwise I lose the resolution of the peaks (high process time=high dead time, lower counts and better resolution). However it is probably the main parameter I'll be able to modify.
I cannot change the apertures.
I could retract the detector, but it is immmobilized in this position and I do not dare to do it. What do you mean by "reenter the geometry of the EDX setup"? Do you mean make a new calibration?
regards,
Stephane
--- Mary Fletcher {maryflet-at-interchange.ubc.ca} wrote:
} Dear Stephane, } It is not a good idea to move the Z axis of the SEM } stage for EDX, this will } upset the geometry of the beam-detector system and } affect quantitation. } There are three possibilities: get an SDD detector, } which is much more } tolerant of high beam currents, use a higher } condenser lens setting (smaller } beam spot size) or a smaller final aperture, if your } SEM has a movable final } aperture. You could also move the EDX detector back } along its track (retract } it), which will change the solid angle but not the } geometry. You may have to } re-enter the geometry in the EDX setup if you do } that. } If you change the HT of the SEM, you may not detect } all the elements. } Good luck, } } Mary Fletcher (nee Mager) } Electron Microscopist } Materials Eng. UBC } #309 - 6350 Stores Road } Vancouver, B.C. V6T 1Z4 } Canada } Tel: 604-822-5648 } Fax: 604-822-3619 } email: maryflet-at-interchange.ubc.ca } } } -----Original Message----- } From: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] } Sent: August 9, 2007 5:54 AM } To: maryflet-at-interchange.ubc.ca } Subject: [Microscopy] question about EDX signal } saturation } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all! } } I am having a too strong signal in EDX (in SEM). } The dead time is usually between 70-80%. } I wondered how I could reduce the signal without } changing the HT. } Changing the size of the beam is one way. } Would it be wise to move the stage in the Z axis to } be } sub-optimal with respect to the EDX detector? } Any other idea? } } Stephane } } } } ____________________________________________________________________________ } ________ } Got a little couch potato? } Check out fun summer activities for kids. } http://search.yahoo.com/search?fr=oni_on_mail&p=summer+activities+for+kids&c } s=bz } } ==============================Original } Headers============================== } 5, 19 -- From nizets2-at-yahoo.com Thu Aug 9 07:44:44 } 2007 } 5, 19 -- Received: from web37403.mail.mud.yahoo.com } (web37403.mail.mud.yahoo.com [209.191.91.135]) } 5, 19 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with SMTP id } l79CiixH016265 } 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 9 } Aug 2007 07:44:44 } -0500 } 5, 19 -- Received: (qmail 52807 invoked by uid } 60001); 9 Aug 2007 12:44:43 } -0000 } 5, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; } c=nofws; } 5, 19 -- s=s1024; d=yahoo.com; } 5, 19 -- } h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Conten } t-Transfer-Encoding:Message-ID; } 5, 19 -- } b=twSYQrDDLa/tW+xKPh1Yn+iU+6fCqgxYvW3XHdl/QAfyM4ORoQ0OfQIoZwsrcpXg43ky3xSKxL } hM90woedzeZ0NHay1zcoR+gWOwula4qVNIOujW5oXuUwSUX4ouilINd5/HxZMSXDncJaclQRpQDm } mbD3S8szeWAsk9qb3y/9Q=; } 5, 19 -- X-YMail-OSG: } CWUsP7oVM1mv2zg2qW3XpSd9xm15WwWQVNQ6WY4qsD3JjeXQYcsXcQSMaciLYDVW9jC0P21_gdeV } Xkj686T_ZB2pykfpEej16rLDeqGcPdUprejITs51nKFp8lDF6w-- } 5, 19 -- Received: from [80.122.101.102] by } web37403.mail.mud.yahoo.com via } HTTP; Thu, 09 Aug 2007 05:44:43 PDT } 5, 19 -- Date: Thu, 9 Aug 2007 05:44:43 -0700 (PDT) } 5, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 5, 19 -- Subject: question about EDX signal } saturation } 5, 19 -- To: microscopy-at-microscopy.com } 5, 19 -- MIME-Version: 1.0 } 5, 19 -- Content-Type: text/plain; } charset=iso-8859-1 } 5, 19 -- Content-Transfer-Encoding: 8bit } 5, 19 -- Message-ID: } {235686.52530.qm-at-web37403.mail.mud.yahoo.com} } ==============================End of - } Headers============================== } }
____________________________________________________________________________________ Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. http://autos.yahoo.com/green_center/
==============================Original Headers============================== 10, 21 -- From nizets2-at-yahoo.com Fri Aug 10 06:10:14 2007 10, 21 -- Received: from web37403.mail.mud.yahoo.com (web37403.mail.mud.yahoo.com [209.191.91.135]) 10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l7ABAEVK017514 10, 21 -- for {microscopy-at-microscopy.com} ; Fri, 10 Aug 2007 06:10:14 -0500 10, 21 -- Received: (qmail 30793 invoked by uid 60001); 10 Aug 2007 11:10:13 -0000 10, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 10, 21 -- s=s1024; d=yahoo.com; 10, 21 -- h=X-YMail-OSG:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 10, 21 -- b=scZZVRJLzAK/8QoO39CTApsMp8EDKoutFh/cfHd9ddrZvv4azCzEthhO73Hu/vGdNDemm5/R8Yo3rn0NQ3MFo1TCj3VMaPYXRWvSSgL063fSjWKpJR+hd6arjTyboj4804XJf2/VD34up00o1xLR/Pj/Yrq5BpUM3zWXfujyYFg=; 10, 21 -- X-YMail-OSG: 0AlivxoVM1mIX.AqLSaBeOkVST_KZk5LGfRMNRgj5J8ayheQzUnIXd0ueZjwgVBASmiqYuylBZZqGVI4V3cy9qsPCZ1r3RiJ8oikvUv6qEbftAXxflsqjtE5JETPppfiOFH1GEh4WdwLzGYFRU4sB74F.MbRbipvuZwiduhzGX0jZEXWB_frViU- 10, 21 -- Received: from [80.122.101.102] by web37403.mail.mud.yahoo.com via HTTP; Fri, 10 Aug 2007 04:10:13 PDT 10, 21 -- Date: Fri, 10 Aug 2007 04:10:13 -0700 (PDT) 10, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 10, 21 -- Subject: RE: [Microscopy] question about EDX signal saturation 10, 21 -- To: maryflet-at-interchange.ubc.ca 10, 21 -- Cc: microscopy-at-microscopy.com 10, 21 -- In-Reply-To: {0JMI007GONLLGE-at-smtp.interchange.ubc.ca} 10, 21 -- MIME-Version: 1.0 10, 21 -- Content-Type: text/plain; charset=iso-8859-1 10, 21 -- Content-Transfer-Encoding: 8bit 10, 21 -- Message-ID: {514655.29726.qm-at-web37403.mail.mud.yahoo.com} ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both David.Llewellyn-at-anu.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: David.Llewellyn-at-anu.edu.au Name: David Llewellyn
Question: Hi all, would anyone have a manual for the thickness coater complete with circuit diagrams, I need to carry out a fix on one of these devices, thanks, David.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both modla-at-dbi.udel.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: modla-at-dbi.udel.edu Name: Shannon Modla
Organization: Delaware Biotechnology Institute
Title-Subject: [Filtered] Beetle Cuticle for TEM
Question: I am attempting to examine beetle cuticle (the wing cover) by TEM but with limited success. In past efforts, I fixed the cuticle overnight in 2.5% glutaraldehyde in sodium cacodylate buffer and post-fixed it in 0.5% osmium tetroxide for 2 hours. Following a standard dehydration in acetone, I slowly infiltrated in Spurr's over the course of a week to ensure full penetration of the resin into the cuticle. I was able to obtain ultrathin sections, but I could not get them to completely flatten. I used both chloroform vapors and a heat pen, but neither would flatten the sections completely.
I know that insect cuticle has a very elastic protein called resilin. I've heard of people dissolving protein from the cuticle by using a KOH solution. I thought that removing this protein could resolve the wrinkle problem, but I am concerned that it would disrupt the ultrastructure.
I was wondering if anyone has had any experience with processing insect cuticle for the TEM and if they could offer any suggestions.
Any feedback would be greatly appreciated!
Sincerely,
Shannon Modla Research Associate Delaware Biotechnology Institute, BioImaging Center 15 Innovation Way Newark, DE 19711
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both teresa.boes-at-hp.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: teresa.boes-at-hp.com Name: Teresa Boes
Organization: Hewlett Packard
Title-Subject: [Filtered] Epoxy in ultra-high vacuum TEM
Question: We are occasionally using Epoxy Bond 110 and sometimes M-Bond 610 to prepare samples and to attach wedge-polished sections to TEM grids. After following the prescribed time and temperature curves for curing, we ion mill and image in an ultra-high vacuum, high resolution TEM. We have more beam contamination on wedge-polished samples than on FIB prepared samples (our most usual method of sample prep), and there is a small, but noticable degredation in vacuum for about a week after imaging wedge-polished samples. We are thinking the contamination and vacuum degredation may be due to an incomplete cure of the epoxy.
Has anyone else had experience with having to adjust adhesive cure times for ultra-high vacuum TEM work? Does anyone have a recommendation for an epoxy that may cure more throughly and cause less contamination? Also, we have always assumed that using as little epoxy as possible is a good thing, but is there a minimum volume necessary for the initiation of cross-linking during the cure?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kerem.uenal-at-uci.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kerem.uenal-at-uci.edu Name: Kerem Unal
Organization: UC Irvine
Title-Subject: [Filtered] two postdoctoral scholar positions at the UC Irvine
Question: Two postdoctoral scholar positions are open in the Prof. Wickramasinghe Lab at the UC Irvine :
- One postdoctoral scholar in the general area of instrument development with a strong background in optics to assist with the development of novel instrumentation for genome sequencing.
http://www.eng.uci.edu/node/1287
- One postdoctoral scholar in the general area of microfabrication with strong background in micromechanics and lithography to assist in the development of micromechanical sensors for novel genome sequencing devices.
http://www.eng.uci.edu/node/1286
Please send your curriculum vitae, a list of publications, and names of at least three references to:
Professor H. Kumar Wickramasinghe Department of Electrical Engineering and Computer Science 325 Engineering Tower University of California, Irvine Irvine, CA 92696-2175
Alternatively, materials may be submitted electronically to: Professor H. Kumar Wickramasinghe at: hkwick-at-uci.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both xiaohutang-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: xiaohutang-at-gmail.com Name: Xiaohu Tang
Organization: Delft University of Technology
Title-Subject: [Filtered] Thin film thickness measurement by SEM/EDX
Question: Dear listeners,
I am trying to measure the thickness of Mo film (about 100nm) on Si substrate by SEM/EDX and Wincasino simulation. From my understanding, Wincasino can simulate a calibration curve of film thickness vs. the x-ray peak ratio of Mo/Si with the same parameters used in experiment (beam energy, TOA, etc.). The real thickness can be located on the calibration curve based on the experimentally measured Mo/Si ratio. It supposed to be an accurate method but I have two questions:
1. Is this method sensitive to the experiment settings (beam energy) and how accurate is it?
2. In Wincasino, which x-ray intensity can be used for peak ratio calculation, absorbed or non-absorbed intensity, or the difference of them? I tried some measurements but they are not promising.
Please also correct me if the procedure I described is wrong.
Dear Teresa, My experience in studying contamination in the SEM was that the contamination was bad for three days after the electron beam hit and "burned" or vapourized the epoxy around a sample. I don't think it was incomplete cure, just that the energy of the beam was high enough to disintegrate the epoxy and put the carbonaceous vapour all through the chamber vacuum. Using as small an amount as possible and keeping the epoxy away from the beam would be my suggestion. Also, you need to mix enough of the components to get the ratios correct and to allow thorough mixing. I find most of my epoxy failures are because of inaccurate ratios or less that complete mixing. Good luck.
-----Original Message----- X-from: teresa.boes-at-hp.com [mailto:teresa.boes-at-hp.com] Sent: August 10, 2007 7:22 AM To: maryflet-at-interchange.ubc.ca
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both teresa.boes-at-hp.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: teresa.boes-at-hp.com Name: Teresa Boes
Organization: Hewlett Packard
Title-Subject: [Filtered] Epoxy in ultra-high vacuum TEM
Question: We are occasionally using Epoxy Bond 110 and sometimes M-Bond 610 to prepare samples and to attach wedge-polished sections to TEM grids. After following the prescribed time and temperature curves for curing, we ion mill and image in an ultra-high vacuum, high resolution TEM. We have more beam contamination on wedge-polished samples than on FIB prepared samples (our most usual method of sample prep), and there is a small, but noticable degredation in vacuum for about a week after imaging wedge-polished samples. We are thinking the contamination and vacuum degredation may be due to an incomplete cure of the epoxy.
Has anyone else had experience with having to adjust adhesive cure times for ultra-high vacuum TEM work? Does anyone have a recommendation for an epoxy that may cure more throughly and cause less contamination? Also, we have always assumed that using as little epoxy as possible is a good thing, but is there a minimum volume necessary for the initiation of cross-linking during the cure?
Xiaohu, If you only need thickness (not composition), this thickness is perfect for x-ray reflectivity measurements and is very accurate because it looks at the constructive/destructive interference pattern. john
At 07:20 AM 8/10/2007, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 6, 19 -- From donovan-at-uoregon.edu Fri Aug 10 12:30:07 2007 6, 19 -- Received: from rwcrmhc15.comcast.net (rwcrmhc15.comcast.net [204.127.192.85]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7AHU6XZ012240 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 10 Aug 2007 12:30:07 -0500 6, 19 -- Message-Id: {200708101730.l7AHU6XZ012240-at-ns.microscopy.com} 6, 19 -- Received: from source.uoregon.edu (c-76-105-219-235.hsd1.or.comcast.net[76.105.219.235]) 6, 19 -- by comcast.net (rwcrmhc15) with SMTP 6, 19 -- id {20070810173005m1500t8cs1e} ; Fri, 10 Aug 2007 17:30:05 +0000 6, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 6, 19 -- Date: Fri, 10 Aug 2007 10:30:04 -0700 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- From: "John J. Donovan" {donovan-at-uoregon.edu} 6, 19 -- Subject: Re: [Microscopy] viaWWW: Thin film thickness measurement by 6, 19 -- SEM/EDX 6, 19 -- Cc: xiaohutang-at-gmail.com 6, 19 -- In-Reply-To: {200708101420.l7AEK2SK019689-at-ns.microscopy.com} 6, 19 -- References: {200708101420.l7AEK2SK019689-at-ns.microscopy.com} 6, 19 -- Mime-Version: 1.0 6, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Be careful interpreting the results. Microanalytical experiments like this measure the mass-thickness not the thickness. If you believe you know the density of your film then you are ok and you can then calculate the thickness. If the film density is different from you assumed density (often bulk densities) then you may run into problems.
Nicholas Ritchie
xiaohutang-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both xiaohutang-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: xiaohutang-at-gmail.com } Name: Xiaohu Tang } } Organization: Delft University of Technology } } Title-Subject: [Filtered] Thin film thickness measurement by SEM/EDX } } Question: Dear listeners, } } I am trying to measure the thickness of Mo film (about 100nm) on Si } substrate by SEM/EDX and Wincasino simulation. From my understanding, } Wincasino can simulate a calibration curve of film thickness vs. the } x-ray peak ratio of Mo/Si with the same parameters used in experiment } (beam energy, TOA, etc.). The real thickness can be located on the } calibration curve based on the experimentally measured Mo/Si ratio. } It supposed to be an accurate method but I have two questions: } } 1. Is this method sensitive to the experiment settings (beam energy) } and how accurate is it? } } 2. In Wincasino, which x-ray intensity can be used for peak ratio } calculation, absorbed or non-absorbed intensity, or the difference of } them? I tried some measurements but they are not promising. } } Please also correct me if the procedure I described is wrong. } } Xiaohu } } Microlab } Delft University of Technology } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 12, 11 -- From zaluzec-at-microscopy.com Fri Aug 10 09:14:08 2007 } 12, 11 -- Received: from [65.213.163.183] (msdvpn8.msd.anl.gov [130.202.238.72]) } 12, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7AEE5ri029239 } 12, 11 -- for {microscopy-at-microscopy.com} ; Fri, 10 Aug 2007 09:14:06 -0500 } 12, 11 -- Mime-Version: 1.0 } 12, 11 -- Message-Id: {p06240804c2e22317d791-at-[65.213.163.183]} } 12, 11 -- Date: Fri, 10 Aug 2007 09:14:16 -0500 } 12, 11 -- To: microscopy-at-microscopy.com } 12, 11 -- From: xiaohutang-at-gmail.com (by way of MicroscopyListserver) } 12, 11 -- Subject: viaWWW: Thin film thickness measurement by SEM/EDX } 12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } }
==============================Original Headers============================== 5, 24 -- From nicholas.ritchie-at-nist.gov Fri Aug 10 12:45:51 2007 5, 24 -- Received: from smtp.nist.gov (rimp1.nist.gov [129.6.16.226]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7AHjnGA024218 5, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Aug 2007 12:45:51 -0500 5, 24 -- Received: from smsd-fw.nist.gov (smsd-fw.nist.gov [129.6.126.23]) 5, 24 -- by smtp.nist.gov (8.13.1/8.13.1) with ESMTP id l7AHjfXH030103; 5, 24 -- Fri, 10 Aug 2007 13:45:41 -0400 5, 24 -- Received: from mag163.nist.gov by smsd-fw.nist.gov 5, 24 -- via smtpd (for webmail.nist.gov [129.6.16.228]) with ESMTP; Fri, 10 Aug 2007 13:45:41 -0400 5, 24 -- Message-ID: {46BCA445.8010108-at-nist.gov} 5, 24 -- Date: Fri, 10 Aug 2007 13:45:41 -0400 5, 24 -- From: "Nicholas W. M. Ritchie" {nicholas.ritchie-at-nist.gov} 5, 24 -- Reply-To: nicholas.ritchie-at-nist.gov 5, 24 -- Organization: N.I.S.T. 5, 24 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 5, 24 -- MIME-Version: 1.0 5, 24 -- To: xiaohutang-at-gmail.com, Microscopy-at-microscopy.com 5, 24 -- Subject: Re: [Microscopy] viaWWW: Thin film thickness measurement by SEM/EDX 5, 24 -- References: {200708101417.l7AEHX3Q009918-at-ns.microscopy.com} 5, 24 -- In-Reply-To: {200708101417.l7AEHX3Q009918-at-ns.microscopy.com} 5, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- X-NIST-MailScanner: Found to be clean 5, 24 -- X-NIST-MailScanner-From: nicholas.ritchie-at-nist.gov ==============================End of - Headers==============================
If I may ask a question, why do you want to measure film thickness using EDX when you can simply take a cross-section and image it? It seems like a long route to the answer, and it won't be very reliable for all the reasons stated on the list. Is this just an experiment or do you plan on using EDX as a method for process control of some kind?
Peter
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both peter.tomic-at-renwireless.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: peter.tomic-at-renwireless.com Name: Peter Tomic
Title-Subject: [Filtered] Measuring Film Thickness With EDX
Question: Question: Dear listeners, } } I am trying to measure the thickness of Mo film (about 100nm) on Si } substrate by SEM/EDX and Wincasino simulation. From my understanding, } Wincasino can simulate a calibration curve of film thickness vs. the } x-ray peak ratio of Mo/Si with the same parameters used in experiment } (beam energy, TOA, etc.). The real thickness can be located on the } calibration curve based on the experimentally measured Mo/Si ratio. } It supposed to be an accurate method but I have two questions: } } 1. Is this method sensitive to the experiment settings (beam energy) } and how accurate is it? } } 2. In Wincasino, which x-ray intensity can be used for peak ratio } calculation, absorbed or non-absorbed intensity, or the difference of } them? I tried some measurements but they are not promising. } } Please also correct me if the procedure I described is wrong. } } Xiaohu
Teresa, I have not used the Epoxy Bond 110. I have used the M-Bond 610 and I don't like it for a number of reasons, but primarily because of its shelf life and the need to be refrigerated. I prefer the Epoxy Technology EpoTek 353ND (www.epotek.com). My trick to make sure that my cross sections are completely cured is to put a drop of epoxy on each Teflon® jaw of my vise that I place on my hot plate. When the drop hardens, I put another drop on the other end of the jaws and wait for those to harden. Then I know that the epoxy that I used is fully cured. It takes longer, but I absolutely know that it is cured.
I suspect that you are right and that you are not getting a full cure. I can't imagine that there is enough material that you expose to the beam to cause a noticeable degradation of the vacuum. Besides, the beam would have a tendency to polymerize anything that it hits into a carbonaceous junk.
-Scott
Scott Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Calejon San Clemente, CA 92673
1-800-728-2233 www.SouthBayTech.com } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both teresa.boes-at-hp.com as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: teresa.boes-at-hp.com } Name: Teresa Boes } } Organization: Hewlett Packard } } Title-Subject: [Filtered] Epoxy in ultra-high vacuum TEM } } Question: We are occasionally using Epoxy Bond 110 and sometimes } M-Bond 610 to prepare samples and to attach wedge-polished sections } to TEM grids. After following the prescribed time and temperature } curves for curing, we ion mill and image in an ultra-high vacuum, } high resolution TEM. We have more beam contamination on } wedge-polished samples than on FIB prepared samples (our most usual } method of sample prep), and there is a small, but noticable } degredation in vacuum for about a week after imaging wedge-polished } samples. We are thinking the contamination and vacuum degredation } may be due to an incomplete cure of the epoxy. } } Has anyone else had experience with having to adjust adhesive cure } times for ultra-high vacuum TEM work? Does anyone have a } recommendation for an epoxy that may cure more throughly and cause } less contamination? Also, we have always assumed that using as } little epoxy as possible is a good thing, but is there a minimum } volume necessary for the initiation of cross-linking during the cure? } } Thanks for your help. } } Teresa } } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Fri Aug 10 09:12:37 2007 } 9, 11 -- Received: from [65.213.163.183] (msdvpn8.msd.anl.gov } [130.202.238.72]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l7AECaep025387 } 9, 11 -- for {microscopy-at-microscopy.com} ; Fri, 10 Aug 2007 09:12:37 -0500 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240802c2e222d2c773-at-[65.213.163.183]} } 9, 11 -- Date: Fri, 10 Aug 2007 09:12:47 -0500 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: teresa.boes-at-hp.com (by way of MicroscopyListserver) } 9, 11 -- Subject: viaWWW: Epoxy in ultra-high vacuum TEM } 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 10, 23 -- From Walck-at-SouthBayTech.com Sat Aug 11 01:46:18 2007 10, 23 -- Received: from wbm7.pair.net (wbm7.pair.net [209.68.4.129]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7B6kHSP030807 10, 23 -- for {microscopy-at-microscopy.com} ; Sat, 11 Aug 2007 01:46:17 -0500 10, 23 -- Received: by wbm7.pair.net (Postfix, from userid 65534) 10, 23 -- id B8D3A1053D; Sat, 11 Aug 2007 02:46:15 -0400 (EDT) 10, 23 -- Received: from 72.197.40.68 ([72.197.40.68]) 10, 23 -- (SquirrelMail authenticated user walck-at-southbaytech.com) 10, 23 -- by webmail7.pair.com with HTTP; 10, 23 -- Fri, 10 Aug 2007 23:46:15 -0700 (PDT) 10, 23 -- Message-ID: {63309.72.197.40.68.1186814775.squirrel-at-webmail7.pair.com} 10, 23 -- In-Reply-To: {200708101417.l7AEH6ut008116-at-ns.microscopy.com} 10, 23 -- References: {200708101417.l7AEH6ut008116-at-ns.microscopy.com} 10, 23 -- Date: Fri, 10 Aug 2007 23:46:15 -0700 (PDT) 10, 23 -- Subject: Re: [Microscopy] viaWWW: Epoxy in ultra-high vacuum TEM 10, 23 -- From: "Scott Walck" {Walck-at-SouthBayTech.com} 10, 23 -- To: teresa.boes-at-hp.com, microscopy-at-microscopy.com 10, 23 -- User-Agent: SquirrelMail/1.4.5 10, 23 -- MIME-Version: 1.0 10, 23 -- Content-Type: text/plain;charset=iso-8859-1 10, 23 -- Content-Transfer-Encoding: 8bit 10, 23 -- X-Priority: 3 (Normal) 10, 23 -- Importance: Normal ==============================End of - Headers==============================
I've got the JSM-35 put together, but I have one last component to connect. It is a "JEOL Beam Interceptor" made by Krisel Control, Inc. It is a digital picoammeter which connects to a black solenoid that inserts a Faraday cup directly under the beam to measure beam current. It has three connectors on the back, labeled "J1-Power" "J2- Beam Current" and "J3- Sample Current."
I have a cable that goes to the J2-Beam Current connector, and this cable goes to a small box with a switch on it, which in turn connects to the solenoid and Faraday cup. Can anyone tell me where the power cable connects to? I have a bunch of the blue "Stick and twist" cable connectors that I can make a cable with, but I need to know where to get the power from, or at least what voltage to use.
The "Sample current" plug is actually using four wires, so I'm not entirely sure how to convert that to connect to the insulated plug on the outside of the chamber door. Again, a pinout or reference would be greatly appreciated.
Thank you,
Justin A. Kraft
==============================Original Headers============================== 5, 26 -- From kraftpiano-at-gmail.com Sat Aug 11 08:28:38 2007 5, 26 -- Received: from wa-out-1112.google.com (wa-out-1112.google.com [209.85.146.183]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7BDSbje017938 5, 26 -- for {microscopy-at-microscopy.com} ; Sat, 11 Aug 2007 08:28:37 -0500 5, 26 -- Received: by wa-out-1112.google.com with SMTP id v27so1261660wah 5, 26 -- for {microscopy-at-microscopy.com} ; Sat, 11 Aug 2007 06:28:36 -0700 (PDT) 5, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 5, 26 -- d=gmail.com; s=beta; 5, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 26 -- b=XwHPvLw+P23P8rdbb1mMAYCUfqhs0UuFLHwByp+RjheMRt+MBOJcNZNqT80AVWOY3hSdCfy5SSlS0JV/HauockhtEbySWOE/0jWwgbozDNeKZ7RNyupQmarODzexNfxFZkE8gSOdISAYrm/+lc+o1JNNmZC8+lVorOUcsmyknag= 5, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 26 -- d=gmail.com; s=beta; 5, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 26 -- b=SVmY5F+w8nhT64ZOdJbmD/nqYEl0BqTCC2BEBFidUzRtpQlYCsifh/vVRmuUXi52FJcA8Q1n9gq+eTH15jA+yv6xGaqK8+i/YhcgAjGDTuWvClX4Dj2oVlABnBu7AXHfDTkpiPAwdIwflVfhuwpvQuIsS5ofCvi2SnvfEdj9y5s= 5, 26 -- Received: by 10.114.111.1 with SMTP id j1mr963457wac.1186838915552; 5, 26 -- Sat, 11 Aug 2007 06:28:35 -0700 (PDT) 5, 26 -- Received: by 10.114.78.15 with HTTP; Sat, 11 Aug 2007 06:28:35 -0700 (PDT) 5, 26 -- Message-ID: {25e2b0d20708110628t7ffd19ceg4b5ae3c3e01f5fe4-at-mail.gmail.com} 5, 26 -- Date: Sat, 11 Aug 2007 09:28:35 -0400 5, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 5, 26 -- To: microscopy-at-microscopy.com 5, 26 -- Subject: JEOL Beam Interceptor wiring 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; charset=ISO-8859-1 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (ZAMTM-at-AOL.COM) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, August 11, 2007 at 10:50:35 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both ZAMTM-at-AOL.COM as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: ZAMTM-at-AOL.COM Name: Michael T. Masnik
Organization: Hobbist
Education: Graduate College
Location: Vienna, VA 22180 USA
Title: Light microscope/digital image
Question: I have over the past couple of years amassed a AO Series 10 phase contrast microscope that gives a great image. I would like to hook it up to a digital camera (I have a triocular head) and be able to download images to my PC. I am an aquatic ecologist and interested in images of aquatic microorganisms. I am at a loss as to what kind, resolution, manufacturer to approach for the digital camera. I want to spend no more than 2 to 3 hundred dollars for the camera an software. Any recommendations, any articles that might help me decide?
There was a serious electrical fire in rooms adjacent to our laboratory. None of our equipment was damaged by heat, water, powder or foam, but many of our electrical and optical components ranging from high end lasers to microscopes and cameras etc. were essentially submerged in a sea of smoke, fumes and soot to varying degrees.
If anybody has personal experience with this, would you please email me off-list or could we please set up a telephone call to discuss how you dealt with this problem.
Thank you.
-Michael
_________________________________________ Michael Cammer http://www.aecom.yu.edu/aif/
==============================Original Headers============================== 8, 35 -- From cammer-at-aecom.yu.edu Sat Aug 11 11:26:35 2007 8, 35 -- Received: from mx1.aecom.yu.edu (mx1.aecom.yu.edu [129.98.1.51]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7BGQY0m011237 8, 35 -- for {microscopy-at-microscopy.com} ; Sat, 11 Aug 2007 11:26:35 -0500 8, 35 -- Received: from draco.aecom.yu.edu (draco.aecom.yu.edu [129.98.1.160]) 8, 35 -- by mx1.aecom.yu.edu (Postfix) with ESMTP id E781E9F001B 8, 35 -- for {microscopy-at-microscopy.com} ; Sat, 11 Aug 2007 12:26:33 -0400 (EDT) 8, 35 -- Received: from draco.aecom.yu.edu (unknown [127.0.0.1]) 8, 35 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 3DFE68B4032 8, 35 -- for {microscopy-at-microscopy.com} ; Sat, 11 Aug 2007 12:15:14 -0400 (EDT) 8, 35 -- X-AuditID: 816201a0-a0ecabb00000765d-aa-46bde09208df 8, 35 -- Received: from post.aecom.yu.edu (post.aecom.yu.edu [129.98.1.100]) 8, 35 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 08756718002 8, 35 -- for {microscopy-at-microscopy.com} ; Sat, 11 Aug 2007 12:15:14 -0400 (EDT) 8, 35 -- Received: from netmail.aecom.yu.edu (netmail2.aecom.yu.edu [129.98.1.59]) 8, 35 -- by post.aecom.yu.edu (Postfix) with ESMTP id 853C720 8, 35 -- for {microscopy-at-microscopy.com} ; Sat, 11 Aug 2007 12:26:33 -0400 (EDT) 8, 35 -- Received: from 162.83.178.52 8, 35 -- (SquirrelMail authenticated user cammer) 8, 35 -- by netmail.aecom.yu.edu with HTTP; 8, 35 -- Sat, 11 Aug 2007 12:24:53 -0400 (EDT) 8, 35 -- Message-ID: {50643.162.83.178.52.1186849493.squirrel-at-netmail.aecom.yu.edu} 8, 35 -- In-Reply-To: {200708111621.l7BGL9mP002691-at-ns.microscopy.com} 8, 35 -- References: {200708111621.l7BGL9mP002691-at-ns.microscopy.com} 8, 35 -- Date: Sat, 11 Aug 2007 12:24:53 -0400 (EDT) 8, 35 -- Subject: recovery after a fire 8, 35 -- From: "Michael Cammer" {cammer-at-aecom.yu.edu} 8, 35 -- To: microscopy-at-microscopy.com 8, 35 -- User-Agent: SquirrelMail/1.4.10a 8, 35 -- MIME-Version: 1.0 8, 35 -- Content-Type: text/plain;charset=iso-8859-1 8, 35 -- Content-Transfer-Encoding: 8bit 8, 35 -- X-Priority: 3 (Normal) 8, 35 -- Importance: Normal 8, 35 -- X-Brightmail-Tracker: AAAAAA== ==============================End of - Headers==============================
The MSA Education Committee has reduced the prices on all Tutorial DVDs. They are now priced at $8.00 for older ones and $15.00 for more recent issues. Get 'em while they are hot! The current catalog can be viewed at : http://www.msa.microscopy.org/MSAUnits/Education/VideoCatalogue.html -- Greg Erdos University of Florida, Retired Micanopy, Florida
==============================Original Headers============================== 1, 23 -- From gwe-at-ufl.edu Sun Aug 12 12:47:46 2007 1, 23 -- Received: from smtp.ufl.edu (smtp02.osg.ufl.edu [128.227.74.165]) 1, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7CHljIO009616 1, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sun, 12 Aug 2007 12:47:46 -0500 1, 23 -- Received: from [10.228.8.76] (ssrb-vpn2-8-76.vpn.ufl.edu [10.228.8.76]) 1, 23 -- (authenticated bits=0) 1, 23 -- by smtp.ufl.edu (8.13.7/8.13.7/2.5.9) with ESMTP id l7CHoj8p1634348 1, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sun, 12 Aug 2007 13:50:46 -0400 1, 23 -- Message-ID: {46BF486D.4060803-at-ufl.edu} 1, 23 -- Date: Sun, 12 Aug 2007 13:50:37 -0400 1, 23 -- From: Greg Erdos {gwe-at-ufl.edu} 1, 23 -- Reply-To: gwe-at-ufl.edu 1, 23 -- User-Agent: Thunderbird 1.5.0.12 (Windows/20070509) 1, 23 -- MIME-Version: 1.0 1, 23 -- To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com} 1, 23 -- Subject: MSA Tutorial DVDs - Prices Slashed 1, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 1, 23 -- Content-Transfer-Encoding: 7bit 1, 23 -- X-Greylist: Sender succeeded SMTP AUTH authentication, not delayed by milter-greylist-3.0rc3 (smtp.ufl.edu [128.227.74.56]); Sun, 12 Aug 2007 13:50:46 -0400 (EDT) 1, 23 -- X-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 1, 23 -- X-UFL-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 1, 23 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 1, 23 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
Hello, Does anyone have data or experience comparing the Osram 103W2 mercury bulb to the Ushio 102D with regards to excitation of GFP?
Thanks, Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
************************************************************************ ****** The box said "Requires WindowsXP or better", so I bought a Macintosh. ************************************************************************ ******
==============================Original Headers============================== 7, 24 -- From glenmac-at-u.washington.edu Sun Aug 12 17:49:31 2007 7, 24 -- Received: from mxout7.cac.washington.edu (mxout7.cac.washington.edu [140.142.32.178]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7CMnUwv027460 7, 24 -- for {microscopy-at-microscopy.com} ; Sun, 12 Aug 2007 17:49:31 -0500 7, 24 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.139]) 7, 24 -- by mxout7.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.06) with ESMTP id l7CMqWKB022500 7, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 7, 24 -- for {microscopy-at-microscopy.com} ; Sun, 12 Aug 2007 15:52:32 -0700 7, 24 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) 7, 24 -- (authenticated authid=glenmac) 7, 24 -- by smtp.washington.edu (8.13.7+UW06.06/8.13.7+UW07.03) with ESMTP id l7CMqVHf007136 7, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 7, 24 -- for {microscopy-at-microscopy.com} ; Sun, 12 Aug 2007 15:52:32 -0700 7, 24 -- Mime-Version: 1.0 (Apple Message framework v752.2) 7, 24 -- Content-Transfer-Encoding: 7bit 7, 24 -- Message-Id: {BB61A11F-CE0B-4FDC-8678-755C3A2ED435-at-u.washington.edu} 7, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 24 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} 7, 24 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 7, 24 -- Subject: Osram vs Ushio 7, 24 -- Date: Sun, 12 Aug 2007 15:52:30 -0700 7, 24 -- X-Mailer: Apple Mail (2.752.2) 7, 24 -- X-PMX-Version: 5.3.3.310218, Antispam-Engine: 2.5.1.298604, Antispam-Data: 2007.8.12.152923 7, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
When this happened to one of our labs we called the equipment manufacturers, arranged to ship the stuff back to them and they cleaned up the internals. It seemed that it was a standard procedure for them. A particular concern was that smoke/soot deposits might lead to electrical short circuits.
All covered by the fire insurance.
cammer-at-aecom.yu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } There was a serious electrical fire in rooms adjacent to our laboratory. } None of our equipment was damaged by heat, water, powder or foam, but many } of our electrical and optical components ranging from high end lasers to } microscopes and cameras etc. were essentially submerged in a sea of smoke, } fumes and soot to varying degrees. } } If anybody has personal experience with this, would you please email me } off-list or could we please set up a telephone call to discuss how you } dealt with this problem. } } Thank you. } } -Michael } } } _________________________________________ } Michael Cammer http://www.aecom.yu.edu/aif/ } } } } ==============================Original Headers============================== } 8, 35 -- From cammer-at-aecom.yu.edu Sat Aug 11 11:26:35 2007 } 8, 35 -- Received: from mx1.aecom.yu.edu (mx1.aecom.yu.edu [129.98.1.51]) } 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7BGQY0m011237 } 8, 35 -- for {microscopy-at-microscopy.com} ; Sat, 11 Aug 2007 11:26:35 -0500 } 8, 35 -- Received: from draco.aecom.yu.edu (draco.aecom.yu.edu [129.98.1.160]) } 8, 35 -- by mx1.aecom.yu.edu (Postfix) with ESMTP id E781E9F001B } 8, 35 -- for {microscopy-at-microscopy.com} ; Sat, 11 Aug 2007 12:26:33 -0400 (EDT) } 8, 35 -- Received: from draco.aecom.yu.edu (unknown [127.0.0.1]) } 8, 35 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 3DFE68B4032 } 8, 35 -- for {microscopy-at-microscopy.com} ; Sat, 11 Aug 2007 12:15:14 -0400 (EDT) } 8, 35 -- X-AuditID: 816201a0-a0ecabb00000765d-aa-46bde09208df } 8, 35 -- Received: from post.aecom.yu.edu (post.aecom.yu.edu [129.98.1.100]) } 8, 35 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 08756718002 } 8, 35 -- for {microscopy-at-microscopy.com} ; Sat, 11 Aug 2007 12:15:14 -0400 (EDT) } 8, 35 -- Received: from netmail.aecom.yu.edu (netmail2.aecom.yu.edu [129.98.1.59]) } 8, 35 -- by post.aecom.yu.edu (Postfix) with ESMTP id 853C720 } 8, 35 -- for {microscopy-at-microscopy.com} ; Sat, 11 Aug 2007 12:26:33 -0400 (EDT) } 8, 35 -- Received: from 162.83.178.52 } 8, 35 -- (SquirrelMail authenticated user cammer) } 8, 35 -- by netmail.aecom.yu.edu with HTTP; } 8, 35 -- Sat, 11 Aug 2007 12:24:53 -0400 (EDT) } 8, 35 -- Message-ID: {50643.162.83.178.52.1186849493.squirrel-at-netmail.aecom.yu.edu} } 8, 35 -- In-Reply-To: {200708111621.l7BGL9mP002691-at-ns.microscopy.com} } 8, 35 -- References: {200708111621.l7BGL9mP002691-at-ns.microscopy.com} } 8, 35 -- Date: Sat, 11 Aug 2007 12:24:53 -0400 (EDT) } 8, 35 -- Subject: recovery after a fire } 8, 35 -- From: "Michael Cammer" {cammer-at-aecom.yu.edu} } 8, 35 -- To: microscopy-at-microscopy.com } 8, 35 -- User-Agent: SquirrelMail/1.4.10a } 8, 35 -- MIME-Version: 1.0 } 8, 35 -- Content-Type: text/plain;charset=iso-8859-1 } 8, 35 -- Content-Transfer-Encoding: 8bit } 8, 35 -- X-Priority: 3 (Normal) } 8, 35 -- Importance: Normal } 8, 35 -- X-Brightmail-Tracker: AAAAAA== } ==============================End of - Headers============================== }
-- AB78-at-ESC.CAM.AC.UK DEPARTMENT OF EARTH SCIENCES UNIVERSITY OF CAMBRIDGE Tel +44 1223 333469 DOWNING STREET +44 1223 333400 CAMBRIDGE FAX +44 1223 333450 CB2 3EQ
Manager of XRD discussion list: http://www.jiscmail.ac.uk/lists/xrd.html
VirtualWDS: For the synthesis of wavelength-dispersive electron probe spectra. http://www.esc.cam.ac.uk/astaff/buckley/VirtualWDS.html
==============================Original Headers============================== 9, 25 -- From ab78-at-esc.cam.ac.uk Mon Aug 13 04:04:58 2007 9, 25 -- Received: from mail.esc.cam.ac.uk (mail.esc.cam.ac.uk [131.111.41.10]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7D94rlU019846 9, 25 -- for {microscopy-at-microscopy.com} ; Mon, 13 Aug 2007 04:04:55 -0500 9, 25 -- Received: from post.esc.cam.ac.uk ([131.111.41.5]) 9, 25 -- by mail.esc.cam.ac.uk with esmtps (TLSv1:AES256-SHA:256) 9, 25 -- (Exim 4.54) 9, 25 -- id 1IKVtx-0002mo-Nx; Mon, 13 Aug 2007 10:07:54 +0100 9, 25 -- Received: from andyb.esc.cam.ac.uk ([192.168.17.155]) 9, 25 -- by post.esc.cam.ac.uk with esmtpsa (TLSv1:AES256-SHA:256) 9, 25 -- (Exim 4.66) 9, 25 -- (envelope-from {ab78-at-esc.cam.ac.uk} ) 9, 25 -- id 1IKVtx-0007iD-49; Mon, 13 Aug 2007 10:07:53 +0100 9, 25 -- Message-ID: {46C01F0F.60503-at-esc.cam.ac.uk} 9, 25 -- Date: Mon, 13 Aug 2007 10:06:23 +0100 9, 25 -- From: Andy Buckley {ab78-at-esc.cam.ac.uk} 9, 25 -- Reply-To: ab78-at-esc.cam.ac.uk 9, 25 -- User-Agent: Thunderbird 1.5.0.12 (Windows/20070509) 9, 25 -- MIME-Version: 1.0 9, 25 -- To: cammer-at-aecom.yu.edu, microscopy-at-microscopy.com 9, 25 -- Subject: Re: [Microscopy] recovery after a fire 9, 25 -- References: {200708111630.l7BGULRK017459-at-ns.microscopy.com} 9, 25 -- In-Reply-To: {200708111630.l7BGULRK017459-at-ns.microscopy.com} 9, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We freeze 4489 all the time. Been doing it for decades with out a problem. Have not noticed any issues with any of the "Re- formulations" (that are sensitive to developing issues). As for condensation the 4489 comes vacuum packed in mylar bags (50/sheets per package), allow them to come to room temp before opening (Completely including the middle of the stack not just the surfaces).
(Remember 4489 must be developed with gas burst agitation).
On 3 Aug 2007 at 11:47, rcommon-at-msu.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Does anyone have experience freezing 4489 TEM film for long-term storage? I } have stored many types of film at -20C with no problems, but haven't tried } it with 4489. Can this cause condensation or other problems? } } Ralph Common } Michigan State University } } } ==============================Original Headers============================== } 4, 24 -- From rcommon-at-msu.edu Fri Aug 3 10:46:40 2007 } 4, 24 -- Received: from sys24.mail.msu.edu (sys24.mail.msu.edu [35.9.75.124]) } 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l73Fke5W016458 } 4, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Aug 2007 10:46:40 -0500 } 4, 24 -- Received: from [35.9.122.125] (helo=emlab) } 4, 24 -- by sys24.mail.msu.edu with esmtpsa (Exim 4.63 #1) } 4, 24 -- (TLSv1:RC4-MD5:128) } 4, 24 -- id 1IGzMN-0001p6-Lo } 4, 24 -- for Microscopy-at-microscopy.com; Fri, 03 Aug 2007 11:46:39 -0400 } 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} } 4, 24 -- To: {Microscopy-at-microscopy.com} } 4, 24 -- Subject: Freezing 4489 Film } 4, 24 -- Date: Fri, 3 Aug 2007 11:48:08 -0400 } 4, 24 -- Message-ID: {000f01c7d5e5$b0c758f0$7d7a0923-at-msu.edu} } 4, 24 -- MIME-Version: 1.0 } 4, 24 -- Content-Type: text/plain; } 4, 24 -- charset="iso-8859-1" } 4, 24 -- Content-Transfer-Encoding: 7bit } 4, 24 -- X-Priority: 3 (Normal) } 4, 24 -- X-MSMail-Priority: Normal } 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) } 4, 24 -- Importance: Normal } 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1896 } 4, 24 -- X-Virus: None found by Clam AV } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 9, 25 -- From edelmare-at-muohio.edu Mon Aug 13 10:14:58 2007 9, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7DFEvAq010922 9, 25 -- for {microscopy-at-Microscopy.com} ; Mon, 13 Aug 2007 10:14:57 -0500 9, 25 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 9, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l7DFHxst004888; 9, 25 -- Mon, 13 Aug 2007 11:17:59 -0400 9, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 9, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l7DFHwTg032153; 9, 25 -- Mon, 13 Aug 2007 11:17:59 -0400 9, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 9, 25 -- To: "rcommon-at-msu.edu" {rcommon-at-msu.edu} 9, 25 -- Date: Mon, 13 Aug 2007 11:17:59 -0400 9, 25 -- MIME-Version: 1.0 9, 25 -- Subject: Re: [Microscopy] Freezing 4489 Film 9, 25 -- CC: microscopy-at-Microscopy.com 9, 25 -- Message-ID: {46C03DE7.7116.B0B80D-at-edelmare.muohio.edu} 9, 25 -- Priority: normal 9, 25 -- In-reply-to: {200708031547.l73FlP7U017636-at-ns.microscopy.com} 9, 25 -- References: {200708031547.l73FlP7U017636-at-ns.microscopy.com} 9, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 9, 25 -- Content-type: text/plain; charset=US-ASCII 9, 25 -- Content-transfer-encoding: 7BIT 9, 25 -- Content-description: Mail message body 9, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
Thanks to all who responded. After receiving answers we went back and beat on Image-J again, and figgured out our mistakes.
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 4, 22 -- From edelmare-at-muohio.edu Mon Aug 13 10:18:09 2007 4, 22 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7DFI85r014903 4, 22 -- for {microscopy-at-Microscopy.com} ; Mon, 13 Aug 2007 10:18:09 -0500 4, 22 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 4, 22 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l7DFLAUw007501 4, 22 -- for {microscopy-at-Microscopy.com} ; Mon, 13 Aug 2007 11:21:10 -0400 4, 22 -- Received: from [192.168.1.23] ([134.53.14.105]) 4, 22 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l7DFLAJ4006852 4, 22 -- for {microscopy-at-Microscopy.com} ; Mon, 13 Aug 2007 11:21:10 -0400 4, 22 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 4, 22 -- To: microscopy-at-Microscopy.com 4, 22 -- Date: Mon, 13 Aug 2007 11:21:10 -0400 4, 22 -- MIME-Version: 1.0 4, 22 -- Subject: RE: FFT Software 4, 22 -- Message-ID: {46C03EA6.24566.B3A496-at-edelmare.muohio.edu} 4, 22 -- Priority: normal 4, 22 -- X-mailer: Pegasus Mail for Windows (4.41) 4, 22 -- Content-type: text/plain; charset=US-ASCII 4, 22 -- Content-transfer-encoding: 7BIT 4, 22 -- Content-description: Mail message body 4, 22 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
From awhitake-at-columbus.rr.com Mon Aug 13 14:39:27 2007 Return-Path: {awhitake-at-columbus.rr.com} Received: from ms-smtp-05.ohiordc.rr.com (ms-smtp-05.ohiordc.rr.com [65.24.5.139]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7DJdRhQ011104; Mon, 13 Aug 2007 14:39:27 -0500 Received: from ms-mss-02 (ms-mss-02-pop-b [10.24.12.35]) by ms-smtp-05.ohiordc.rr.com (8.13.6/8.13.6) with ESMTP id l7DJgQwO017130; Mon, 13 Aug 2007 15:42:27 -0400 (EDT) Received: from columbus.rr.com (localhost [127.0.0.1]) by ms-mss-02.columbus.rr.com (iPlanet Messaging Server 5.2 HotFix 2.10 (built Dec 26 2005)) with ESMTP id {0JMQ0027X9EQR8-at-ms-mss-02.columbus.rr.com} ; Mon, 13 Aug 2007 15:42:26 -0400 (EDT) Received: from [10.24.12.105] (Forwarded-For: [125.244.13.2]) by ms-mss-02.columbus.rr.com (mshttpd); Tue, 14 Aug 2007 03:42:26 +0800
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both slc6-at-lehigh.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: slc6-at-lehigh.edu Name: Sharon Coe
Organization: Lehigh University Department of Materials Science & Engineering
Title-Subject: [Filtered] Faculty Position in Analytical Electron Microscopy Lehigh University
Question: Lehigh University (Bethlehem, PA) seeks to fill a tenure track position at the level of Assistant or Associate Professor in Materials Science and Engineering. The department is searching for an outstanding individual who develops new analytical electron microscopy (AEM) techniques and applies these methodologies to solve cutting edge nanocharacterization problems in the fields of Materials Science, Nanotechnology or Electronic Materials. An earned doctorate is required, as well as demonstrated ability in teaching and research. The successful candidate will be responsible for teaching undergraduate and graduate courses in the Materials Science and Engineering curriculum, and establishing a vibrant, high-quality research program. This will include participation in multidisciplinary activities such as those coordinated by the Center for Advanced Materials and Nanotechnology, the International Materials Institute for New Functionality in Glasses, the Sherman Fairchild Center for Solid State Physics, and the Center for Optical Technologies. The Nanocharacterization Laboratory at Lehigh University has an excellent suite of electron-optical instrumentation (www.lehigh.edu/~inmicro) including two state-of-the-art aberration corrected analytical electron microscopes, and is home to the world renowned Lehigh Microscopy School. The successful applicant would be expected to provide leadership in the area of aberration corrected AEM and to champion its application to the study of interfaces on the nanoscale. A strong desire to perform interdisciplinary research and a willingness to collaborate across departmental boundaries are essential. Please submit a CV by October 30, 2007, along with a Teaching Proposal describing instructional philosophy and interests at undergraduate and graduate levels, and a 3-6 page Research Proposal describing an externally fundable research program, to Sharon Coe, Lehigh University, 5 E. Packer Ave., Bethlehem, PA 18015-3195. Lehigh is committed to recruiting, retaining and ! tenuring women and members of minority groups.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both xiaohutang-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: xiaohutang-at-gmail.com Name: Xiaohu Tang
Organization: TU Delft
Title-Subject: [Filtered] Secondary electron yield detection
Question: Hello Listeners,
Here I want to in-situ monitor the secondary electron yield under e-beam irradiation. Could anybody recommend one such SE detector or instrument with such function? Another question is what the extent of gas pressure change (say, changes from 10E-6 to 10E-8mbar) affects SE yield and the detector readings. Thank you.
The College of Microscopy located in Westmont, IL will be offering the following electron microscopy short courses this fall:
September 25-27 - Transmission Electron Microscopy
October 15-19 - Scanning Electron Microscopy
In addition to lectures, these courses emphasize hands-on training using state of the art equipment. For further details and registration information, please follow the link below.
www.collegeofmicroscopy.com
Elaine Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 11, 27 -- From eschumacher-at-mccrone.com Tue Aug 14 08:32:20 2007 11, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7EDWJCS028409 11, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 14 Aug 2007 08:32:20 -0500 11, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 11, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id B212D1A8015 11, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 14 Aug 2007 08:35:22 -0500 (CDT) 11, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 11, 27 -- by pgp.mccrone.com (PGP Universal service); 11, 27 -- Tue, 14 Aug 2007 08:35:22 -0500 11, 27 -- X-PGP-Universal: processed 11, 27 -- Content-class: urn:content-classes:message 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- charset="us-ascii" 11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 27 -- Subject: Short Course Announcement: TEM and SEM 11, 27 -- Date: Tue, 14 Aug 2007 08:35:16 -0500 11, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A7B97715-at-MCCRONEMSG.tmg.mccrone.com} 11, 27 -- X-MS-Has-Attach: 11, 27 -- X-MS-TNEF-Correlator: 11, 27 -- Thread-Topic: Short Course Announcement: TEM and SEM 11, 27 -- Thread-Index: Acfed/LZ+/9zTMbORyODvNbpV1/FCw== 11, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 11, 27 -- To: {Microscopy-at-MSA.Microscopy.Com} 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7EDWJCS028409 ==============================End of - Headers==============================
I have begun to build a database of examples for use in teaching TEM and STEM. This project grew out of my need for classroom and homework examples from techniques that I do not employ in my own research. My hobby-horse that TEM measurements should be treated as data, not just pictures, left me unsatisfied with scanning images out of journals, and the result is now on the web at
http://tem.msae.wisc.edu/emdb/
Please take a look. The coverage at the moment is limited to materials-oriented TEM and STEM, since those are my areas of expertise, and remains spotty even within that realm. I therefor also appeal for contributions to the database, especially interesting EDS spectra and diffraction contrast images of crystal defects. For those of you in the US, this is an easy way to leverage "broader impact" from your NSF-funded research: simply send me an interesting but unpublished example from your research. Instructions on how to contribute are on the web site under "About EMdb".
When you access the database, you will be asked for your email address. This is used only as an easy-to-remember unique identifier so that I can do some rudimentary tracking of the database use for the purpose of reporting to NSF.
Please direct any questions, comments, and contributions to me.
Best wishes, Paul Voyles
Paul Voyles Materials Science and Engineering University of Wisconsin, Madison 1509 University Ave, Rm 223 Madison, WI 53706-1595 voice: (608) 265-6740 fax: (608) 262-8353 voyles-at-engr.wisc.edu http://tem.msae.wisc.edu
==============================Original Headers============================== 8, 21 -- From voyles-at-engr.wisc.edu Tue Aug 14 20:44:16 2007 8, 21 -- Received: from mail.cae.wisc.edu (mail.cae.wisc.edu [144.92.13.31]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7F1iF67031659 8, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 14 Aug 2007 20:44:16 -0500 8, 21 -- Received: from smtpauth.cae.wisc.edu (smtpauth.cae.wisc.edu [144.92.13.83]) 8, 21 -- by mail.cae.wisc.edu (8.13.7+Sun/8.13.3) with ESMTP id l7F1lGAP019192 8, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 14 Aug 2007 20:47:16 -0500 (CDT) 8, 21 -- Received: from [192.168.1.101] ([76.210.74.234]) 8, 21 -- (authenticated bits=0) 8, 21 -- by smtpauth.cae.wisc.edu (8.13.4/8.13.4/Debian-3sarge2) with ESMTP id l7F1lAA3026052 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 14 Aug 2007 20:47:16 -0500 8, 21 -- Message-ID: {46C25B18.7040809-at-engr.wisc.edu} 8, 21 -- Date: Tue, 14 Aug 2007 20:47:04 -0500 8, 21 -- From: Paul Voyles {voyles-at-engr.wisc.edu} 8, 21 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 8, 21 -- MIME-Version: 1.0 8, 21 -- To: Microscopy-at-MSA.Microscopy.Com 8, 21 -- Subject: database of teaching examples for TEM and STEM 8, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both reznik-at-ict.uni-karlsruhe.de as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: reznik-at-ict.uni-karlsruhe.de Name: Boris Reznik
Organization: University of Karlsruhe,Germany
Title-Subject: [Filtered] FFT_Software
Question: I am looking for a simple free-software allowing FFT-Analysis of HRTEM images. Any recommendations that might help me? Thank you
Depending on what you want to do, NIH image will do FFT. The price is right! David
On Aug 15, 2007, at 6:30 AM, reznik-at-ict.uni-karlsruhe.de wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } ---------------------------------------------------------------------- } ----- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both reznik-at-ict.uni-karlsruhe.de as well as the } MIcroscopy Listserver } ---------------------------------------------------------------------- } ----- } } Email: reznik-at-ict.uni-karlsruhe.de } Name: Boris Reznik } } Organization: University of Karlsruhe,Germany } } Title-Subject: [Filtered] FFT_Software } } Question: I am looking for a simple free-software allowing FFT- } Analysis of HRTEM images. Any recommendations that might help me? } Thank you } } } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Wed Aug 15 08:26:15 2007 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id l7FDQD2U027379 } 7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 15 Aug 2007 } 08:26:15 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240800c2e8b018a436-at-[206.69.208.22]} } 7, 11 -- Date: Wed, 15 Aug 2007 08:29:15 -0500 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: reznik-at-ict.uni-karlsruhe.de (by way of } MicroscopyListserver) } 7, 11 -- Subject: viaWWW: FFT_Software } 7, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers============================== }
==============================Original Headers============================== 5, 22 -- From Elliott-at-arizona.edu Wed Aug 15 09:23:09 2007 5, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.132.44]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7FEN8LV008028 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 15 Aug 2007 09:23:09 -0500 5, 22 -- Received: from gandalfs_amavis (amavis6.email.arizona.edu [10.0.0.209]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 21EA9BB91E 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 15 Aug 2007 07:26:08 -0700 (MST) 5, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id E8B4AC27EA 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 15 Aug 2007 07:26:05 -0700 (MST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 5, 22 -- In-Reply-To: {200708151330.l7FDU7w7032312-at-ns.microscopy.com} 5, 22 -- References: {200708151330.l7FDU7w7032312-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {170E4270-5515-4926-9516-707E52C0DB12-at-arizona.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: David Elliott {Elliott-at-arizona.edu} 5, 22 -- Subject: Re: [Microscopy] viaWWW: FFT_Software 5, 22 -- Date: Wed, 15 Aug 2007 07:26:02 -0700 5, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 22 -- X-Mailer: Apple Mail (2.752.2) 5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
We are continuing to purge some of our non-used equipment and have the following items available for sale.
1. 3 Reichert OM3 ultramicrotomes, still in great shape and fully functional. 2. 1 Reichert OM2 ultramicrotome, still in great shape and fully functional 3. 1 Sorvall ultramicrotome 4. 1 Spencer 820 microtome, great for thick sections on resins like JB4 5. a variety of compound microscopes for sale (mostly Zeiss) and a variety of lenses to accompany 6. we still have the beautiful orange Zeiss EM10C for sale. With it comes a second 10C to be used for parts. A great deal at only $10K (canadian dollars to boot), no it does not include shipping.
Look for more great deals in the near future. Give me a call 604-822-3354 for prices and a list of the compound scopes for sale.
-- Garnet Martens
Research Manager BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
phone 604-822-3354
==============================Original Headers============================== 8, 27 -- From gmartens-at-interchange.ubc.ca Wed Aug 15 12:49:37 2007 8, 27 -- Received: from mr6.mail-relay.ubc.ca (mr6.mail-relay.ubc.ca [137.82.45.11]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7FHnb0i025631 8, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 15 Aug 2007 12:49:37 -0500 8, 27 -- X-Ubc-Received: from mr6.mail-relay.ubc.ca (localhost [127.0.0.1]) 8, 27 -- by localhost (Postfix) with SMTP id B160915B7E 8, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 15 Aug 2007 10:52:38 -0700 (PDT) 8, 27 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 8, 27 -- by mr6.mail-relay.ubc.ca (Postfix) with ESMTP 8, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 15 Aug 2007 10:52:34 -0700 (PDT) 8, 27 -- Received: from [137.82.85.216] (0511.emlab.ubc.ca [137.82.85.216]) 8, 27 -- by smtp.interchange.ubc.ca 8, 27 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 8, 27 -- with ESMTPA id {0JMT00406TNLBF-at-smtp.interchange.ubc.ca} for 8, 27 -- Microscopy-at-MSA.Microscopy.Com; Wed, 15 Aug 2007 10:52:34 -0700 (PDT) 8, 27 -- Date: Wed, 15 Aug 2007 10:53:23 -0700 8, 27 -- From: Garnet Martens {gmartens-at-interchange.ubc.ca} 8, 27 -- Subject: equipment for sale 8, 27 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 8, 27 -- Message-id: {a06240805c2e8ebe866fc-at-[137.82.85.216]} 8, 27 -- MIME-version: 1.0 8, 27 -- Content-type: text/plain; format=flowed; charset=us-ascii 8, 27 -- X-UBC-Scanned: Sophos PureMessage 5.3.2.304607, Antispam-Engine: 2.5.1.298604, Antispam-Data: 2007.8.15.102722 8, 27 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 8, 27 -- X-PerlMx-Spam: Probability=7%, Report=__C230066_P5 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_7 0, __pbl.spamhaus.org_TIMEOUT , __sbl.spamhaus.org_TIMEOUT 8, 27 -- X-Spam-Level: 8, 27 -- X-Spam-Flag: No ==============================End of - Headers==============================
Nanotechnology Research Spurs Growth in the Global Microscopes Market Source:PRNewswire Author:n/a
Global consulting firm Frost & Sullivan has released new analysis indicating that nanotechnology applications in bioscience and material science research are likely to increase demand for microscopic imaging and analysis systems and contribute to a rise in the global microscope market's revenues from US$1.87 billion in 2006 to US$3.54 billion in 2013. Frost & Sullivan research analyst Lakshman Koundinya said: "With the continued miniaturization of semiconductors and electronicproducts, there exists a growing need for easier and more accurate material inspection. Consequently, there has been a significant increase in funds allocated for research into fields such as nanotechnology and nanosciences, thereby creating significant demand opportunities for microscopes." The article says growth in the microscope market, however, is limited by a lack of technological innovation and other factors that have resulted in the rise of other analytical techniques. The article also says that high cost of manufacturing and significant research and development investment requirements can create barriers to entry in the microscope market. Koundinya said: "Since financial limitations hamper manufacturers' response to the industry's continued technology advancements, consolidation in certain industries such as the disk drive industry has resulted in fewer manufacturers with high financial stability." The article says that manufacturers must create more user-friendly and easy to operate microscopes in order to compensate for a decline in technical expertise. The article can be viewed online at the link below.
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
This message is from pH2. This message and any attachments may contain legally privileged or confidential information, and are intended only for the individual or entity identified above as the addressee. If you are not the addressee, or if this message has been addressed to you in error, you are not authorized to read, copy, or distribute this message and any attachments, and we ask that you please delete this message and attachments (including all copies) and notify the sender by return e-mail or by phone at 317-718-7020. Delivery of this message and any attachments to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or a privilege. All personal messages express views only of the sender, which are not to be attributed to pH2 and may not be copied or distributed without this statement.
==============================Original Headers============================== 11, 26 -- From ph2-at-sprynet.com Thu Aug 16 00:48:07 2007 11, 26 -- Received: from elasmtp-mealy.atl.sa.earthlink.net (elasmtp-mealy.atl.sa.earthlink.net [209.86.89.69]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7G5m7ET021175 11, 26 -- for {microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 00:48:07 -0500 11, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 26 -- s=dk20050327; d=sprynet.com; 11, 26 -- b=MHsbIgl4zzarlfoUE1RpnXxB5E17L8ni5QgcjM+7/tM13wO/03iValgwcdEGl1ho; 11, 26 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-MimeOLE:thread-index:Message-ID:X-ELNK-Trace:X-Originating-IP; 11, 26 -- Received: from [68.73.147.81] (helo=user915fa8f284) 11, 26 -- by elasmtp-mealy.atl.sa.earthlink.net with asmtp (Exim 4.34) 11, 26 -- id 1ILYGC-0000k0-Gv 11, 26 -- for microscopy-at-microscopy.com; Thu, 16 Aug 2007 01:51:08 -0400 11, 26 -- From: "Tony Havics" {ph2-at-sprynet.com} 11, 26 -- To: "Micrscopy Listserve" {microscopy-at-microscopy.com} 11, 26 -- Subject: FYI - Nanotechnology Research Spurs Growth in the Global Microscopes Market 11, 26 -- Date: Thu, 16 Aug 2007 01:51:06 -0400 11, 26 -- MIME-Version: 1.0 11, 26 -- Content-Type: text/plain; 11, 26 -- charset="us-ascii" 11, 26 -- Content-Transfer-Encoding: 7bit 11, 26 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 11, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 11, 26 -- thread-index: AcffyW/b0pl3VcZtQiSWjVaIkVZtIw== 11, 26 -- Message-ID: {E1ILYGC-0000k0-Gv-at-elasmtp-mealy.atl.sa.earthlink.net} 11, 26 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f9cef6f3fc19ec20d069506bd425779789350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 11, 26 -- X-Originating-IP: 68.73.147.81 ==============================End of - Headers==============================
I am looking for a reasonably priced, reasonable quality polarizing microscope to round out my equipment for my class. Right now I have a quote for a Leica DM EP, but I thought I'd ask if anyone has a recommendation on other brands that have relatively good optics.
I am also looking for a reasonable digital camera to use for the purpose of sharing live microscope images via a data projector. Any recommendations on models there would be helpful as well. I've looked into the Kenavision scope cameras, but I've heard that the goose neck stand can be a little unwieldy at times.
I'm trying to get the best balance between budget and quality. I don't want an instrument that is cheaply made (As a good portion of the "school grade" microscopes are) but I don't want to break the bank on a $9K instrument.
Thanks in advance for your recommendations,
Justin A. Kraft
==============================Original Headers============================== 5, 26 -- From kraftpiano-at-gmail.com Thu Aug 16 06:49:34 2007 5, 26 -- Received: from wa-out-1112.google.com (wa-out-1112.google.com [209.85.146.178]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7GBnX2f009018 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 06:49:34 -0500 5, 26 -- Received: by wa-out-1112.google.com with SMTP id v27so261728wah 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 04:52:35 -0700 (PDT) 5, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 5, 26 -- d=gmail.com; s=beta; 5, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 26 -- b=uAcdmqF2qnIlBuzSwAsoGlkiG4CZwbJ0fXyACud5HIhzjBuyUeKsKxmgv7zC6IrNqw4vXONnuZkLX3+l3XU4MAQMZXvf78O0NGBUE3ZzRwEwAq5dkffkOcjComJXZqEJ5FNB39JaMBokZ2wBXGfxnO5FM1OtmfH8GNSrnjW4drY= 5, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 26 -- d=gmail.com; s=beta; 5, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 26 -- b=G0nOGlJPcB0MosQ5qyFXZjjPWFwMjvCcFMw6Npb/fb+HD0Ewtj7HyvozGto4uvbQPrvt3jhDv9AXBYcVLj9/MLiEjqUGXUo7WLS1g+JC7aeVxWJGObZDZLkkfcCJd7zYQWyb0TyJpTBzwrpxdRispTNITV/Vdmbae3T/beJAbYI= 5, 26 -- Received: by 10.114.200.2 with SMTP id x2mr1042331waf.1187265154759; 5, 26 -- Thu, 16 Aug 2007 04:52:34 -0700 (PDT) 5, 26 -- Received: by 10.114.92.5 with HTTP; Thu, 16 Aug 2007 04:52:34 -0700 (PDT) 5, 26 -- Message-ID: {25e2b0d20708160452o1da407ecr5865afbfbcb8520f-at-mail.gmail.com} 5, 26 -- Date: Thu, 16 Aug 2007 07:52:34 -0400 5, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 5, 26 -- To: microscopy-at-microscopy.com 5, 26 -- Subject: Polarizing microscope recommendation. 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; charset=ISO-8859-1 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
Dear Group: does anyone know somebody up in the Savannah area that would be able to give us a value of two instruments that will be given as a tax donation? Currently both instruments are not running. Please contact me ASAP, via email redsponger-at-yahoo.com or call me on my cell (954) 401-4542. Thanks so much,
1. JEOL JEM 1200EX II - TEM 2. ISI DS-130S - SEM w/PGT EDS and Image Analysis System
Barbara Maloney FIU/FCAEM 11200 SW 8th Street PC50 Miami, FL 33199 (305) 348-2714 Fax (305) 348-3580
==============================Original Headers============================== 4, 19 -- From maloneyb-at-fiu.edu Thu Aug 16 07:35:52 2007 4, 19 -- Received: from fmailhost03.isp.att.net (fmailhost03.isp.att.net [204.127.217.103]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7GCZqKW021891 4, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 07:35:52 -0500 4, 19 -- Received: from [192.168.1.2] (adsl-074-166-189-137.sip.mia.bellsouth.net[74.166.189.137]) 4, 19 -- by bellsouth.net (frfwmhc03) with ESMTP 4, 19 -- id {20070816123853H0300svfaue} ; Thu, 16 Aug 2007 12:38:53 +0000 4, 19 -- X-Originating-IP: [74.166.189.137] 4, 19 -- Message-ID: {46C4455A.3040904-at-fiu.edu} 4, 19 -- Date: Thu, 16 Aug 2007 08:38:50 -0400 4, 19 -- From: barbara maloney {maloneyb-at-fiu.edu} 4, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 4, 19 -- X-Accept-Language: en-us, en 4, 19 -- MIME-Version: 1.0 4, 19 -- To: Microscopy-at-microscopy.com 4, 19 -- Subject: Need of evaluation of two instruments - TEM and SEM 4, 19 -- X-Priority: 2 (High) 4, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Group: does anyone know somebody up in the Savannah area that would be able to give us a value of two instruments that will be given as a tax donation after viewing the instruments? Currently both instruments are not running. Please contact me ASAP, via email redsponger-at-yahoo.com or call me on my cell (954) 401-4542. Thanks so much,
1. JEOL JEM 1200EX II - TEM 2. ISI DS-130S - SEM w/PGT EDS and Image Analysis System
Barbara Maloney FIU/FCAEM 11200 SW 8th Street PC50 Miami, FL 33199 (305) 348-2714 Fax (305) 348-3580
==============================Original Headers============================== 4, 18 -- From maloneyb-at-fiu.edu Thu Aug 16 07:59:59 2007 4, 18 -- Received: from fmailhost02.isp.att.net (fmailhost02.isp.att.net [207.115.11.52]) 4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7GCxwpj001760 4, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 07:59:58 -0500 4, 18 -- Received: from [192.168.1.2] (adsl-074-166-189-137.sip.mia.bellsouth.net[74.166.189.137]) 4, 18 -- by bellsouth.net (frfwmhc02) with ESMTP 4, 18 -- id {20070816130259H02005m7qqe} ; Thu, 16 Aug 2007 13:02:59 +0000 4, 18 -- X-Originating-IP: [74.166.189.137] 4, 18 -- Message-ID: {46C44B00.60300-at-fiu.edu} 4, 18 -- Date: Thu, 16 Aug 2007 09:02:56 -0400 4, 18 -- From: barbara maloney {maloneyb-at-fiu.edu} 4, 18 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 4, 18 -- X-Accept-Language: en-us, en 4, 18 -- MIME-Version: 1.0 4, 18 -- To: Microscopy-at-microscopy.com 4, 18 -- Subject: Need an evaluation of a TEM and SEM in the Savannah, Georgia area 4, 18 -- Content-Type: text/plain; charset=us-ascii; format=flowed 4, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
have a look at Meiji microscopes (japanese brand) and Lumenera Infinitiy cameras. Both have very good quality and the price is mid ranged.
If you need more information you can contact me off-line.
Regards Anneliese opto-at-klughammer.de
2007/8/16, kraftpiano-at-gmail.com {kraftpiano-at-gmail.com} : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I am looking for a reasonably priced, reasonable quality polarizing } microscope to round out my equipment for my class. Right now I have a } quote for a Leica DM EP, but I thought I'd ask if anyone has a } recommendation on other brands that have relatively good optics. } } I am also looking for a reasonable digital camera to use for the } purpose of sharing live microscope images via a data projector. Any } recommendations on models there would be helpful as well. I've looked } into the Kenavision scope cameras, but I've heard that the goose neck } stand can be a little unwieldy at times. } } I'm trying to get the best balance between budget and quality. I } don't want an instrument that is cheaply made (As a good portion of } the "school grade" microscopes are) but I don't want to break the bank } on a $9K instrument. } } Thanks in advance for your recommendations, } } Justin A. Kraft } } ==============================Original Headers============================== } 5, 26 -- From kraftpiano-at-gmail.com Thu Aug 16 06:49:34 2007 } 5, 26 -- Received: from wa-out-1112.google.com (wa-out-1112.google.com [209.85.146.178]) } 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7GBnX2f009018 } 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 06:49:34 -0500 } 5, 26 -- Received: by wa-out-1112.google.com with SMTP id v27so261728wah } 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 04:52:35 -0700 (PDT) } 5, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; } 5, 26 -- d=gmail.com; s=beta; } 5, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 5, 26 -- b=uAcdmqF2qnIlBuzSwAsoGlkiG4CZwbJ0fXyACud5HIhzjBuyUeKsKxmgv7zC6IrNqw4vXONnuZkLX3+l3XU4MAQMZXvf78O0NGBUE3ZzRwEwAq5dkffkOcjComJXZqEJ5FNB39JaMBokZ2wBXGfxnO5FM1OtmfH8GNSrnjW4drY= } 5, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 5, 26 -- d=gmail.com; s=beta; } 5, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 5, 26 -- b=G0nOGlJPcB0MosQ5qyFXZjjPWFwMjvCcFMw6Npb/fb+HD0Ewtj7HyvozGto4uvbQPrvt3jhDv9AXBYcVLj9/MLiEjqUGXUo7WLS1g+JC7aeVxWJGObZDZLkkfcCJd7zYQWyb0TyJpTBzwrpxdRispTNITV/Vdmbae3T/beJAbYI= } 5, 26 -- Received: by 10.114.200.2 with SMTP id x2mr1042331waf.1187265154759; } 5, 26 -- Thu, 16 Aug 2007 04:52:34 -0700 (PDT) } 5, 26 -- Received: by 10.114.92.5 with HTTP; Thu, 16 Aug 2007 04:52:34 -0700 (PDT) } 5, 26 -- Message-ID: {25e2b0d20708160452o1da407ecr5865afbfbcb8520f-at-mail.gmail.com} } 5, 26 -- Date: Thu, 16 Aug 2007 07:52:34 -0400 } 5, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} } 5, 26 -- To: microscopy-at-microscopy.com } 5, 26 -- Subject: Polarizing microscope recommendation. } 5, 26 -- MIME-Version: 1.0 } 5, 26 -- Content-Type: text/plain; charset=ISO-8859-1 } 5, 26 -- Content-Transfer-Encoding: 7bit } 5, 26 -- Content-Disposition: inline } ==============================End of - Headers============================== }
I suggest you contact John Mackenzie at North Carolina State University for suggestions on cameras. He is a wealth of information on all aspects of digital imaging. See his address in copy list.
Best regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Happiness equals reality minus expectations." Tom Magliozzi
opto-at-klughamme r.de To gary.m.brown-at-exxonmobil.com 08/16/07 08:06 cc AM Subject [Microscopy] Re: Polarizing Please respond microscope recommendation. to opto-at-klughamme r.de
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------------
} } I am looking for a reasonably priced, reasonable quality polarizing } microscope to round out my equipment for my class. Right now I have a } quote for a Leica DM EP, but I thought I'd ask if anyone has a } recommendation on other brands that have relatively good optics. } } I am also looking for a reasonable digital camera to use for the } purpose of sharing live microscope images via a data projector. Any } recommendations on models there would be helpful as well. I've looked } into the Kenavision scope cameras, but I've heard that the goose neck } stand can be a little unwieldy at times. } } I'm trying to get the best balance between budget and quality. I } don't want an instrument that is cheaply made (As a good portion of } the "school grade" microscopes are) but I don't want to break the bank } on a $9K instrument. } } Thanks in advance for your recommendations, } } Justin A. Kraft } } ==============================Original Headers============================== } 5, 26 -- From kraftpiano-at-gmail.com Thu Aug 16 06:49:34 2007 } 5, 26 -- Received: from wa-out-1112.google.com (wa-out-1112.google.com [209.85.146.178]) } 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7GBnX2f009018 } 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 06:49:34 -0500 } 5, 26 -- Received: by wa-out-1112.google.com with SMTP id v27so261728wah } 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 04:52:35 -0700 (PDT) } 5, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; } 5, 26 -- d=gmail.com; s=beta; } 5, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition;
We have done this inexpensively (~$2.00) by buying sheets of polarizing material from Edmunds Scientific. We cut a square that we mount on the condenser, and a circle that we insert somewhere in the light path. You then rotate the condenser polarizer until you get extinction. Unless you want more sophistication, this works quite well for things like crystalline materials and even muscle fibers. It does suck up light though.
Joel
Date sent: Thu, 16 Aug 2007 06:49:42 -0500 To: jbs-at-temple.edu X-from: kraftpiano-at-gmail.com Send reply to: kraftpiano-at-gmail.com
Dear Group - thanks so much for your quick responses - I have found someone to do this. Thanks so much. Barbara
==============================Original Headers============================== 4, 18 -- From maloneyb-at-fiu.edu Thu Aug 16 11:31:40 2007 4, 18 -- Received: from smtp9.fiu.edu (smtp9.fiu.edu [131.94.189.202]) 4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7GGVeBt025693 4, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 11:31:40 -0500 4, 18 -- Received: from esg197d.fiu.edu (EHLO _131.94.220.36_) (131.94.220.36) 4, 18 -- by smtp9.fiu.edu (MOS 3.7.1-GA FastPath queued) 4, 18 -- with ESMTP id EPD93011; 4, 18 -- Thu, 16 Aug 2007 12:34:41 -0400 (EDT) 4, 18 -- Message-ID: {46C47D22.7080301-at-fiu.edu} 4, 18 -- Date: Thu, 16 Aug 2007 12:36:50 -0400 4, 18 -- From: Barbara Maloney {maloneyb-at-fiu.edu} 4, 18 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 4, 18 -- X-Accept-Language: en-us, en 4, 18 -- MIME-Version: 1.0 4, 18 -- To: Microscopy-at-microscopy.com 4, 18 -- Subject: Evaluation of instruments 4, 18 -- Content-Type: text/plain; charset=us-ascii; format=flowed 4, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We are in need of an uninterrupted power system(UPS) for our high-end Field Emission SEM.
I would appreciate if anyone could suggest a good one with reasonable price. The main purpose of the UPS for us is to keep the SEM running when there is a power outage.
Thanks
Yan Xin NHMFL/FSU Tallahassee, FL
==============================Original Headers============================== 6, 23 -- From xin-at-magnet.fsu.edu Thu Aug 16 14:42:14 2007 6, 23 -- Received: from mail.magnet.fsu.edu (mail.magnet.fsu.edu [146.201.250.9]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7GJgEoG011914 6, 23 -- for {microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 14:42:14 -0500 6, 23 -- Received: from xinlt.magnet.fsu.edu (xinlt.ad.magnet.fsu.edu [146.201.233.35] (may be forged)) 6, 23 -- (authenticated bits=0) 6, 23 -- by mail.magnet.fsu.edu (8.13.8/8.13.6) with ESMTP id l7GJjBOO005038 6, 23 -- for {microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 15:45:11 -0400 6, 23 -- Message-Id: {7.0.0.16.2.20070816153935.024e7c38-at-magnet.fsu.edu} 6, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.0.16 6, 23 -- Date: Thu, 16 Aug 2007 15:45:05 -0400 6, 23 -- To: microscopy-at-microscopy.com 6, 23 -- From: Yan Xin {xin-at-magnet.fsu.edu} 6, 23 -- Subject: recommendation for a UPS 6, 23 -- Mime-Version: 1.0 6, 23 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 23 -- X-NHMFL-MailScanner-Information: Please contact CSG for more information 6, 23 -- X-NHMFL-MailScanner: Found to be clean 6, 23 -- X-MailScanner-MCPCheck: 6, 23 -- X-NHMFL-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, 6, 23 -- score=-5.8, required 5, autolearn=not spam, ALL_TRUSTED -1.80, 6, 23 -- BAYES_00 -4.00) 6, 23 -- X-MailScanner-From: xin-at-magnet.fsu.edu ==============================End of - Headers==============================
I highly recommend Liebert Nfinity double conversion UPS. These can have redundant conversion modules (4KVA each) and redundant control modules. At a full load of batteries, each unit (I have two identical units) has a backup run time of 288 minutes at 22% load. SEM, EDS, EBSD, other PCs are on one unit. Chiller and air compressor are on another unit.
Loaded, each unit costs about $10K and are well worth it. No sag, no drop outs, no sweat. They can also be monitored via HTTP web browser.
gary g.
At 11:47 AM 8/16/2007, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Thu Aug 16 15:19:57 2007 10, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l7GKJuI8024517 10, 20 -- for {microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 15:19:57 -0500 10, 20 -- Message-Id: {200708162019.l7GKJuI8024517-at-ns.microscopy.com} 10, 20 -- Received: (qmail 22951 invoked from network); 16 Aug 2007 13:22:57 -0700 10, 20 -- Received: by simscan 1.1.0 ppid: 22948, pid: 22949, t: 0.0912s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp3 with SMTP; 16 Aug 2007 13:22:57 -0700 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Thu, 16 Aug 2007 13:22:58 -0800 10, 20 -- To: xin-at-magnet.fsu.edu 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] recommendation for a UPS 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200708161947.l7GJlWp0014770-at-ns.microscopy.com} 10, 20 -- References: {200708161947.l7GJlWp0014770-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-16A129CB ==============================End of - Headers==============================
We have an older JEOL 840 SEM with a Kevex Sigma setup that we use to acquire digital images. This system seems to have broken - the images on the compter are distorted and noisy, so we want to replace it. Do any of you have opinions as to what system we should get (or not)? We just need digital imaging capability on this instrument - we're not looking to get the EDX back up.
Thanks in advance,
Andy Bowling
==============================Original Headers============================== 5, 30 -- From Andrew.Bowling-at-ARS.USDA.GOV Thu Aug 16 15:39:22 2007 5, 30 -- Received: from messagescreen4.ars.usda.gov (messagescreen4.ars.usda.gov [199.133.180.151]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7GKdLUk004050 5, 30 -- for {microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 15:39:22 -0500 5, 30 -- Received: from CO-MAILBH-01.ARSNET.ARS.USDA.GOV ([199.133.183.226]) 5, 30 -- by messagescreen4.ars.usda.gov (8.13.8/8.13.8) with ESMTP id l7GKgNF7030880 5, 30 -- for {microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 15:42:23 -0500 5, 30 -- Received: from CO-MAIL-03.ARSNET.ARS.USDA.GOV ([10.100.2.202]) by CO-MAILBH-01.ARSNET.ARS.USDA.GOV with Microsoft SMTPSVC(6.0.3790.3959); 5, 30 -- Thu, 16 Aug 2007 14:42:22 -0600 5, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 30 -- Content-class: urn:content-classes:message 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Type: text/plain; 5, 30 -- charset="us-ascii" 5, 30 -- Subject: SEM image acquisition hardware recommendations 5, 30 -- Date: Thu, 16 Aug 2007 14:42:22 -0600 5, 30 -- Message-ID: {8017F94146BF634DA9414E4B9088525B256DEB-at-CO-MAIL-03.ARSNET.ARS.USDA.GOV} 5, 30 -- X-MS-Has-Attach: 5, 30 -- X-MS-TNEF-Correlator: 5, 30 -- Thread-Topic: SEM image acquisition hardware recommendations 5, 30 -- Thread-Index: AcfgTbArtSfZ+ZitR1u7ujjyYsS9yw== 5, 30 -- From: "Bowling, Andrew" {Andrew.Bowling-at-ARS.USDA.GOV} 5, 30 -- To: {microscopy-at-microscopy.com} 5, 30 -- X-OriginalArrivalTime: 16 Aug 2007 20:42:22.0915 (UTC) FILETIME=[F27F6930:01C7E045] 5, 30 -- X-MessageScreenMessageID: 1187296943.762174.1254.3029434380 5, 30 -- X-MessageScreenContentScore: Score of 0 assigned to Content 5, 30 -- X-MessageScreenUCEScore: Score of 0 assigned to UCE 5, 30 -- X-MessageScreen: Analyzed by IntelliReach MessageScreen(tm) 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7GKdLUk004050 ==============================End of - Headers==============================
We've been very pleased with our 4pi system on our older Hitachi S570 SEM.
} We have an older JEOL 840 SEM with a Kevex Sigma setup that we use to } acquire digital images. This system seems to have broken - the images } on the compter are distorted and noisy, so we want to replace it. Do } any of you have opinions as to what system we should get (or not)? We } just need digital imaging capability on this instrument - we're not } looking to get the EDX back up.
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sirapa-at-optonline.net as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sirapa-at-optonline.net Name: Alan Paris
Organization: Leica Microsystems
Title-Subject: [Filtered] Metal Samples for Microscopy
Question: I am looking to purchase a set of reference polished metal samples for reflected light microscopy.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both alternate.questions-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: Hi All: I am attempting to stain mitochondria from PRP (using CD41 to stain platelets and looking for an efficient stain for the mitos). Any ideas what to use for minimum background and maximum affinity/efficiency?
I wish I could answer your question. I'd like to know myself. Buehler used to make some very nice sets, but I think they stopped making them. I've seen them from time to time come up for auction on ebay. I have also seen some coming out of Bangalore, India that appear to be newly made. I couldn't find them right now looking on google. I have made quite a few for my own use in house, I don't know what kind of facilities you have to do this. It's a fair amount of work, but it depends upon how many alloys you need to have.
Do let me know what you find out.
Good luck.
dj
On Thu, 16 Aug 2007, sirapa-at-optonline.net wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both sirapa-at-optonline.net as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: sirapa-at-optonline.net } Name: Alan Paris } } Organization: Leica Microsystems } } Title-Subject: [Filtered] Metal Samples for Microscopy } } Question: I am looking to purchase a set of reference polished metal samples for reflected light microscopy. } } Can anyone refer me to a source? } Thank you } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Thu Aug 16 22:04:36 2007 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7H34ZLG005977 } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 16 Aug 2007 22:04:35 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240801c2eac169b573-at-[206.69.208.22]} } 7, 11 -- Date: Thu, 16 Aug 2007 22:07:35 -0500 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: sirapa-at-optonline.net (by way of Nestor J. Zaluzec) } 7, 11 -- Subject: viaWWW: Metal Samples for Light Microscopy } 7, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 19 -- From dljones-at-bestweb.net Fri Aug 17 07:56:59 2007 8, 19 -- Received: from vms046pub.verizon.net (vms046pub.verizon.net [206.46.252.46]) 8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7HCuxDw013632 8, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Aug 2007 07:56:59 -0500 8, 19 -- Received: from localhost ([71.247.97.221]) 8, 19 -- by vms046.mailsrvcs.net (Sun Java System Messaging Server 6.2-6.01 (built Apr 8, 19 -- 3 2006)) with ESMTPA id {0JMX00C2V5F3RFJ3-at-vms046.mailsrvcs.net} for 8, 19 -- Microscopy-at-microscopy.com; Fri, 17 Aug 2007 07:59:41 -0500 (CDT) 8, 19 -- Date: Fri, 17 Aug 2007 09:01:37 -0400 (Eastern Daylight Time) 8, 19 -- From: "David L. Jones" {dljones-at-bestweb.net} 8, 19 -- Subject: Re: [Microscopy] viaWWW: Metal Samples for Light Microscopy 8, 19 -- In-reply-to: {200708170313.l7H3DwpL017888-at-ns.microscopy.com} 8, 19 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 8, 19 -- To: sirapa-at-optonline.net 8, 19 -- Cc: Microscopy-at-microscopy.com 8, 19 -- Message-id: {Pine.WNT.4.64.0708170852580.3484-at-H-F1} 8, 19 -- MIME-version: 1.0 8, 19 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 8, 19 -- References: {200708170313.l7H3DwpL017888-at-ns.microscopy.com} ==============================End of - Headers==============================
The College of Microscopy located in Westmont, IL, will offer a course in Raman microspectroscopy October 2-4, 2007, designed to provide practical instruction in "real world" use of the Raman microscope. The class will utilize demonstrations and laboratory exercises supplemented with lectures. The role of Raman microspectroscopy in the overall scheme of industrial problem solving will be addressed. Students are strongly encouraged to bring their own samples for analysis. Class size is limited to eight students to allow for maximum participation.
For further details and registration information, please follow the link below.
www.collegeofmicroscopy.com
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
==============================Original Headers============================== 11, 27 -- From eschumacher-at-mccrone.com Fri Aug 17 09:13:38 2007 11, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7HEDcrj006468 11, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 17 Aug 2007 09:13:38 -0500 11, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 11, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 850051A8016 11, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 17 Aug 2007 09:16:41 -0500 (CDT) 11, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 11, 27 -- by pgp.mccrone.com (PGP Universal service); 11, 27 -- Fri, 17 Aug 2007 09:16:41 -0500 11, 27 -- X-PGP-Universal: processed 11, 27 -- Content-class: urn:content-classes:message 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- charset="us-ascii" 11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 27 -- Subject: Short Course Announcement: Raman Microspectroscopy 11, 27 -- Date: Fri, 17 Aug 2007 09:16:33 -0500 11, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A7B97729-at-MCCRONEMSG.tmg.mccrone.com} 11, 27 -- X-MS-Has-Attach: 11, 27 -- X-MS-TNEF-Correlator: 11, 27 -- Thread-Topic: Short Course Announcement: Raman Microspectroscopy 11, 27 -- Thread-Index: Acfg2Tb08xlFuYOjSS+D6mEy1xLJSA== 11, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 11, 27 -- To: {Microscopy-at-MSA.Microscopy.Com} 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7HEDcrj006468 ==============================End of - Headers==============================
The Midwest Microscopy and Microanalysis Society will celebrate our 50th anniversary with a meeting on September 18 and 19, 2007, to be held at the College of Microscopy in Westmont, IL. Details, registration information and a preliminary program can be found on our website under Meetings:
www.midwestmicroscopy.org
Details will be updated on the website as plans are finalized. We hope to see you there!
Elaine Schumacher President, Midwest Microscopy and Microanalysis Society
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
==============================Original Headers============================== 12, 27 -- From eschumacher-at-mccrone.com Fri Aug 17 13:24:54 2007 12, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7HIOs21023205 12, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 17 Aug 2007 13:24:54 -0500 12, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 12, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id C819C1A8016 12, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 17 Aug 2007 13:27:56 -0500 (CDT) 12, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 12, 27 -- by pgp.mccrone.com (PGP Universal service); 12, 27 -- Fri, 17 Aug 2007 13:27:56 -0500 12, 27 -- X-PGP-Universal: processed 12, 27 -- Content-class: urn:content-classes:message 12, 27 -- MIME-Version: 1.0 12, 27 -- Content-Type: text/plain; 12, 27 -- charset="us-ascii" 12, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 27 -- Subject: Meeting Announcement: Midwest Microscopy & Microanalysis Society 50th Anniversary Event 12, 27 -- Date: Fri, 17 Aug 2007 13:27:49 -0500 12, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A7B9772D-at-MCCRONEMSG.tmg.mccrone.com} 12, 27 -- X-MS-Has-Attach: 12, 27 -- X-MS-TNEF-Correlator: 12, 27 -- Thread-Topic: Meeting Announcement: Midwest Microscopy & Microanalysis Society 50th Anniversary Event 12, 27 -- Thread-Index: Acfg/FEVlOi5bweUQj+NU40uCPDCsA== 12, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 12, 27 -- To: {Microscopy-at-MSA.Microscopy.Com} 12, 27 -- Content-Transfer-Encoding: 8bit 12, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7HIOs21023205 ==============================End of - Headers==============================
I would like to ask for your advice on cutting K4M lowicryl and assessing the polymerized blocks. It is my first time to cut lowicryls embedded in low temperature. I embedded drosophila tissue samples using the supplier's recommended schedule for low temperature embedding. Samples were fixed in 4% paraformaldehyde, 0.2% glutaraldehyde in 0.1M Phosphate buffer, dehydrated in progressive lowering of temperature (PLT) in ethanol, infiltrated and embedded with monostep K4M lowicryl. The resin and sample were cured in a commercial UV cryochamber at -35 degree celsius.
The blocks are hard but I noticed during trimming with a razor blade that it is softer than what I usually deal with in Epon sections. In resin areas close to the trimmed pyramid block face, I can see a slight indentation left after pressing my nail on the resin. I was able to cut 1.2 micron thick sections although it compressed at some areas and later flattened out. The color of the thick sections is not uniform throughout. I get pink, green colors randomly throughout the sections. When I tried to collect thin sections, the sections come with horizontal shreds and do not get a full section. I tried to cut 50-100 nm at 5mm/s then to 2mm/s hoping I could get a full section with these softer blocks. The water in the trough is low enough that kept the diamond knife edge wet. A few times I got water on the block surface, wicked it off with kimwipe and proceeded in sectioning.
Is there a way to make the polymerized blocks harder? When a drop of water got in contact with the trimmed block face, is it necessary to dry it by putting in dessicator before sectioning? I plan on using this resin in the near future with PLT dehydration and UV cryopolymerization. I would like to hear your suggestions to have better sectioning next time.
I look forward to your helpful advice.
Thank you for the time to read my email.
Sincerely,
Claire
____________________________________________________________________________________ Pinpoint customers who are looking for what you sell. http://searchmarketing.yahoo.com/
==============================Original Headers============================== 11, 19 -- From lukeclaire-at-yahoo.com Sat Aug 18 11:11:45 2007 11, 19 -- Received: from web55310.mail.re4.yahoo.com (web55310.mail.re4.yahoo.com [206.190.58.189]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l7IGBi9b028169 11, 19 -- for {Microscopy-at-microscopy.com} ; Sat, 18 Aug 2007 11:11:44 -0500 11, 19 -- Received: (qmail 65265 invoked by uid 60001); 18 Aug 2007 16:14:46 -0000 11, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 19 -- s=s1024; d=yahoo.com; 11, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 11, 19 -- b=wxEberEscGWdTZk6v35hpkjrE9wMrHDixoolcu6zArd9CYKolrtcJKVEfWmgVUFwVGZgyZFESmSJjBLXDN6EpIqu1dVRZviPkB5QN1j1ECPKX/roAy/nzZeWsbybIZasndokhI6bvtO6V8VbvGMZyJkrgWy2B6m+vODH1WICWOo=; 11, 19 -- X-YMail-OSG: d_K.m2oVM1khuoVdrjyALXKoHkpMpGnZZOTfWYLKHpKVKz27MKAUvqTQhCZeW31bdw-- 11, 19 -- Received: from [128.249.119.233] by web55310.mail.re4.yahoo.com via HTTP; Sat, 18 Aug 2007 09:14:45 PDT 11, 19 -- Date: Sat, 18 Aug 2007 09:14:45 -0700 (PDT) 11, 19 -- From: claire haueter {lukeclaire-at-yahoo.com} 11, 19 -- Subject: Monostep K4M lowicryl for Low Embed Polymerization 11, 19 -- To: Microscopy-at-microscopy.com 11, 19 -- MIME-Version: 1.0 11, 19 -- Content-Type: text/plain; charset=iso-8859-1 11, 19 -- Content-Transfer-Encoding: 8bit 11, 19 -- Message-ID: {994532.64543.qm-at-web55310.mail.re4.yahoo.com} ==============================End of - Headers==============================
Within days of the posting, I received a call from Gatan to help me diagnose the malfunction. A flex cable between the CCD and the camera head electronics was probably cracked such that connection was open only when the camera was inserted and the cable was stretched. This cable is part of the carrier module that slides back and forth holding the CCD. Gatan will actually sell this module (about $3K) for field replacement by the user. But with no other means to further diagnose this 12-yr old camera, I opted to send the camera back to Gatan for repair. I have the camera back this past friday and it's performing fine.
According to Gatan, this flex cable should last about 7 years or so and that certainly will depend on how often the camera is inserted and retracted. If anybody is interested in seeing the inside of this camera, I have some pictures that I can put up on our website. Thanks for all who offered help and advice.
-- Pang (Wai Pang Chan, wpchan-at-u.washington.edu, PAB A087, 206-685-1519) The Biology Imaging Facility (http://staff.washington.edu/wpchan/if/)
On Tue, 17 Jul 2007, wpchan-at-u.washington.edu wrote:
} I encountered a strange problem with a Gatan 689 Slow-Scan CCD camera } mounted in the 35 mm port above the viewing chamber of a Philips CM100 } TEM. When I insert the camera, I can only see a uniform grey image. The } histogram shows a single line in the middle. Changing the exposure time } or increasing the illumination via condenser 2 has no effect. Allowing } the shutter to be normally closed, or putting the shutter to open also has } no effect. } } There seemed to be no mechanical problem for inserting or retracting the } camera because the beam was blocked and blank as it should be. } } We are still using DigitalMicrograph 2.5 on an old quadra 840 with system } 7.1. I have trashed the preferences and used another copy of the software } with no improvement. When the camera is out, I can see the raw image in } unprocessed view; basically the dark reference image. With high tension } off, I used to see the same image when I insert the camera. But now I } only see a grey image. I don't think there is anything wrong with the CCD } or scintillator because I can see an after-image of the image I should be } seeing when I retract the camera. This suggested that the CCD was } exposed and formed an image but somehow not transferred to the computer. } } Any suggestion or insight to solve this problem will be much appreciated.
==============================Original Headers============================== 5, 22 -- From wpchan-at-u.washington.edu Sat Aug 18 18:58:22 2007 5, 22 -- Received: from mxout5.cac.washington.edu (mxout5.cac.washington.edu [140.142.32.135]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7INwL8D016333 5, 22 -- for {microscopy-at-microscopy.com} ; Sat, 18 Aug 2007 18:58:22 -0500 5, 22 -- Received: from homer24.u.washington.edu (homer24.u.washington.edu [140.142.15.10]) 5, 22 -- by mxout5.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.06) with ESMTP id l7J01ML4013653 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 5, 22 -- for {microscopy-at-microscopy.com} ; Sat, 18 Aug 2007 17:01:22 -0700 5, 22 -- Received: from localhost (wpchan-at-localhost) 5, 22 -- by homer24.u.washington.edu (8.13.7+UW06.06/8.13.7+Submit) with ESMTP id l7J01Msp023139 5, 22 -- for {microscopy-at-microscopy.com} ; Sat, 18 Aug 2007 17:01:22 -0700 5, 22 -- Date: Sat, 18 Aug 2007 17:01:22 -0700 (PDT) 5, 22 -- From: "W. Chan" {wpchan-at-u.washington.edu} 5, 22 -- To: microscopy-at-microscopy.com 5, 22 -- Subject: Re: [Microscopy] gatan camera 5, 22 -- In-Reply-To: {200707180047.l6I0lgM0012695-at-ns.microscopy.com} 5, 22 -- Message-ID: {Pine.LNX.4.64.0708101008430.12136-at-homer21.u.washington.edu} 5, 22 -- References: {200707180047.l6I0lgM0012695-at-ns.microscopy.com} 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 5, 22 -- X-PMX-Version: 5.3.3.310218, Antispam-Engine: 2.5.1.298604, Antispam-Data: 2007.8.18.163024 5, 22 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_7 0' ==============================End of - Headers==============================
Late but hoping not too late ! I come back from holliday...
A way to "glue" without carbon which has not been cited, is to use as substrate an Indium foil. In is very soft, and one can press the powder in the In film. In is conductive too, if not oxyded. Depending of the grain size of the powder, one may have overlapping from In-L lines with elements from the samples such as Ca-K, but for a carbon search, it should work. It's a way much used in XPS analysis of powders, in UHV environement, where glue outgas to much.
And as In is expensive, it's easy to save monney by re-melt it (in a glas tube) to purify it and separate the last used powder, and laminate it again.
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
nizets2-at-yahoo.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all! } } I am trying to detect organic contamination on/in } aluminosilicate material by SEM+EDX, so it is very } important for me to eliminate any source of carbon. } As the specimen holders are made whether of carbon, } silicium or aluminium (what a luck ;-)), I am planning } to use copper disks as support but I still have to fix } the powder on the disks. Is there anywhere in the } known universe a glue which does not contain carbon? } } Best regards, } } Stephane } } } } ____________________________________________________________________________________ } Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. } http://mobile.yahoo.com/go?refer=1GNXIC } } ==============================Original Headers============================== } 6, 19 -- From nizets2-at-yahoo.com Thu Jul 26 09:32:01 2007 } 6, 19 -- Received: from web37410.mail.mud.yahoo.com (web37410.mail.mud.yahoo.com [209.191.91.142]) } 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l6QEW1ww005035 } 6, 19 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jul 2007 09:32:01 -0500 } 6, 19 -- Received: (qmail 60447 invoked by uid 60001); 26 Jul 2007 14:32:01 -0000 } 6, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 6, 19 -- s=s1024; d=yahoo.com; } 6, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; } 6, 19 -- b=2MiVhOx+6j1t7m2e81pHb/jHTgXzbUmsYUA+yk1XZSWcjjf/BVBEv3o+e4XTMSlxilxZFzkrVsUD56VjH+n9+/aF0MnNXNGnWRC9s07qKLVBJ26t6HOotcIzJ8khtKlduR62uXpvWWDrANn6iQv0v1UG5sxOeq6vf4PBNUXNXDs=; } 6, 19 -- X-YMail-OSG: pwv8uE8VM1mA.9IHjDcSfAD1ePW6QFPm9bVPXiNfmGtQv9ZinXCUW2go8o4xOmCTAGJjxK3u1gku8f0sXkOa4sGhRLluvvj8b0mHk3JPkqxahMHLWtT2NH9aHCFPFA-- } 6, 19 -- Received: from [80.122.101.102] by web37410.mail.mud.yahoo.com via HTTP; Thu, 26 Jul 2007 07:32:01 PDT } 6, 19 -- Date: Thu, 26 Jul 2007 07:32:01 -0700 (PDT) } 6, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 6, 19 -- Subject: Glue without carbon (SEM) } 6, 19 -- To: microscopy-at-microscopy.com } 6, 19 -- MIME-Version: 1.0 } 6, 19 -- Content-Type: text/plain; charset=iso-8859-1 } 6, 19 -- Content-Transfer-Encoding: 8bit } 6, 19 -- Message-ID: {223471.59362.qm-at-web37410.mail.mud.yahoo.com} } ==============================End of - Headers============================== }
==============================Original Headers============================== 10, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Mon Aug 20 07:37:27 2007 10, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.155]) 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7KCbPVe019364 10, 29 -- for {microscopy-at-microscopy.com} ; Mon, 20 Aug 2007 07:37:26 -0500 10, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 10, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id l7KChKY6006287 10, 29 -- ; Mon, 20 Aug 2007 14:43:20 +0200 (CEST) 10, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 10, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id B37E33EC01C; 10, 29 -- Mon, 20 Aug 2007 14:42:36 +0200 (CEST) 10, 29 -- Message-ID: {46C98C3E.2030309-at-ipcms.u-strasbg.fr} 10, 29 -- Date: Mon, 20 Aug 2007 14:42:38 +0200 10, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 10, 29 -- User-Agent: Thunderbird 1.5.0.12 (X11/20070604) 10, 29 -- MIME-Version: 1.0 10, 29 -- To: nizets2-at-yahoo.com, Microscopy-at-microscopy.com 10, 29 -- Subject: Re: [Microscopy] Glue without carbon (SEM) 10, 29 -- References: {200707261438.l6QEcQXv013950-at-ns.microscopy.com} 10, 29 -- In-Reply-To: {200707261438.l6QEcQXv013950-at-ns.microscopy.com} 10, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 29 -- Content-Transfer-Encoding: 8bit 10, 29 -- X-IPCMS-MailScanner: Found to be clean 10, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 10, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.155]); Mon, 20 Aug 2007 14:43:21 +0200 (CEST) 10, 29 -- X-Virus-Scanned: ClamAV 0.88.7/4010/Mon Aug 20 13:56:24 2007 on mr5.u-strasbg.fr 10, 29 -- X-Virus-Status: Clean 10, 29 -- X-Spam-Status: No, score=-0.0 required=5.0 tests=AWL autolearn=disabled 10, 29 -- version=3.1.8 10, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr5.u-strasbg.fr ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both chao.wang-at-materials.ox.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: chao.wang-at-materials.ox.ac.uk Name: Chao Wang
Organization: Department of Materials, Oxford
Title-Subject: [Filtered] estimate roughness of HREM images
Question: Dear All
Could anyone tell me how to estimate the roughness of epitaxial layers by MBE growth like the roughness between Fe and MgO,from both HREM images and ADF images?
There are two main concerns. 1. From HREM, it's hard to see the where is the interface sometime. 2. Because the layer are epitaxial, how to estimate several Amstrong roughness?
The Microscopy Core Facility I am directing is heavily funded by a couple of NIH institutional grants. As a result, I am able to keep most of my microscopes updated and people pay less user fees. I need to, however, to have the PIs acknowledge these grants in their publications.
I keep sending letters to the PIs to request this but without great success. I am sure I am not alone and I wonder how other people/facilities do this?
Thank you for your help.
Zhaojie Zhang, Ph.D. Director, Microscopy Core Facility Department of Zoology and Physiology University of Wyoming Laramie, WY 82071 TEL: 307-766-3038 FAX: 307-766-5625 zzhang-at-uwyo.edu http://www.uwyo.edu/microscopy
==============================Original Headers============================== 8, 26 -- From ZZhang-at-uwyo.edu Mon Aug 20 12:43:08 2007 8, 26 -- Received: from willowsprings.uwyo.edu (willowsprings.uwyo.edu [129.72.10.31]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7KHh8wD018435 8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Aug 2007 12:43:08 -0500 8, 26 -- Received: from UWMAIL.uwyo.edu (uwmail.uwyo.edu [172.26.4.76]) 8, 26 -- by willowsprings.uwyo.edu (8.13.8/8.13.8) with ESMTP id l7KHnAcH021021 8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Aug 2007 11:49:11 -0600 (MDT) 8, 26 -- (envelope-from ZZhang-at-uwyo.edu) 8, 26 -- Received: from TELEGRAPH5.uwyo.edu ([10.84.60.120]) by UWMAIL.uwyo.edu with Microsoft SMTPSVC(6.0.3790.1830); 8, 26 -- Mon, 20 Aug 2007 11:47:50 -0600 8, 26 -- x-mimeole: Produced By Microsoft Exchange V6.5 8, 26 -- Content-class: urn:content-classes:message 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-Type: text/plain; 8, 26 -- charset="US-ASCII" 8, 26 -- Subject: 8, 26 -- Date: Mon, 20 Aug 2007 11:47:49 -0600 8, 26 -- Message-ID: {C9C1AF307F12AF4087DEF87CB0E6A4AD01A3803D-at-TELEGRAPH5.uwyo.edu} 8, 26 -- X-MS-Has-Attach: 8, 26 -- X-MS-TNEF-Correlator: 8, 26 -- Thread-Index: AcfjUjm7eKmZJ3t1SZalWtImdvI4xw== 8, 26 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} 8, 26 -- To: {Microscopy-at-microscopy.com} 8, 26 -- X-OriginalArrivalTime: 20 Aug 2007 17:47:50.0837 (UTC) FILETIME=[3A4E1E50:01C7E352] 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7KHh8wD018435 ==============================End of - Headers==============================
My first thought upon reading your question was "good luck getting people to acknowledge your facility in their papers", but then I started thinking. Would it work to send a letter out saying that unacknowledged work will be retroactively billed to labs at the 'unsubsidized' (MUCH higher) rate? Just make the case that if they want subsidies they have to help get them.
I don't know if it would work as I have not tried it, but I think I will try it.
David
_____________________
David Elliott Ph.D. Assistant Professor - Department of Cell Biology and Anatomy Director, Research Microscopy Core Service University of Arizona College of Medicine PO Box 245004 Tucson, AZ 85724
Voice: 520-626-7870 Fax: 520-626-2097
On Aug 20, 2007, at 10:47 AM, ZZhang-at-uwyo.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi everyone: } } The Microscopy Core Facility I am directing is heavily funded by a } couple of NIH institutional grants. As a result, I am able to keep } most } of my microscopes updated and people pay less user fees. I need to, } however, to have the PIs acknowledge these grants in their } publications. } } I keep sending letters to the PIs to request this but without great } success. I am sure I am not alone and I wonder how other } people/facilities do this? } } Thank you for your help. } } } } Zhaojie Zhang, Ph.D. } Director, Microscopy Core Facility } Department of Zoology and Physiology } University of Wyoming } Laramie, WY 82071 } TEL: 307-766-3038 } FAX: 307-766-5625 } zzhang-at-uwyo.edu } http://www.uwyo.edu/microscopy } } } } } } ==============================Original } Headers============================== } 8, 26 -- From ZZhang-at-uwyo.edu Mon Aug 20 12:43:08 2007 } 8, 26 -- Received: from willowsprings.uwyo.edu } (willowsprings.uwyo.edu [129.72.10.31]) } 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id l7KHh8wD018435 } 8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Aug 2007 } 12:43:08 -0500 } 8, 26 -- Received: from UWMAIL.uwyo.edu (uwmail.uwyo.edu } [172.26.4.76]) } 8, 26 -- by willowsprings.uwyo.edu (8.13.8/8.13.8) with ESMTP id } l7KHnAcH021021 } 8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Aug 2007 } 11:49:11 -0600 (MDT) } 8, 26 -- (envelope-from ZZhang-at-uwyo.edu) } 8, 26 -- Received: from TELEGRAPH5.uwyo.edu ([10.84.60.120]) by } UWMAIL.uwyo.edu with Microsoft SMTPSVC(6.0.3790.1830); } 8, 26 -- Mon, 20 Aug 2007 11:47:50 -0600 } 8, 26 -- x-mimeole: Produced By Microsoft Exchange V6.5 } 8, 26 -- Content-class: urn:content-classes:message } 8, 26 -- MIME-Version: 1.0 } 8, 26 -- Content-Type: text/plain; } 8, 26 -- charset="US-ASCII" } 8, 26 -- Subject: } 8, 26 -- Date: Mon, 20 Aug 2007 11:47:49 -0600 } 8, 26 -- Message-ID: } {C9C1AF307F12AF4087DEF87CB0E6A4AD01A3803D-at-TELEGRAPH5.uwyo.edu} } 8, 26 -- X-MS-Has-Attach: } 8, 26 -- X-MS-TNEF-Correlator: } 8, 26 -- Thread-Index: AcfjUjm7eKmZJ3t1SZalWtImdvI4xw== } 8, 26 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} } 8, 26 -- To: {Microscopy-at-microscopy.com} } 8, 26 -- X-OriginalArrivalTime: 20 Aug 2007 17:47:50.0837 (UTC) } FILETIME=[3A4E1E50:01C7E352] } 8, 26 -- Content-Transfer-Encoding: 8bit } 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id l7KHh8wD018435 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 12, 22 -- From Elliott-at-arizona.edu Mon Aug 20 13:17:37 2007 12, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7KIHacr030672 12, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 20 Aug 2007 13:17:37 -0500 12, 22 -- Received: from gandalfs_amavis (amavis3.email.arizona.edu [10.0.0.206]) 12, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 5A9B1C4EEC 12, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 20 Aug 2007 11:23:39 -0700 (MST) 12, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 12, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 9CA27C4882 12, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 20 Aug 2007 11:23:36 -0700 (MST) 12, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 12, 22 -- In-Reply-To: {200708201747.l7KHlDO2023302-at-ns.microscopy.com} 12, 22 -- References: {200708201747.l7KHlDO2023302-at-ns.microscopy.com} 12, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 12, 22 -- Message-Id: {DD3A1BDD-B3DA-40F7-92BD-15F564C9ED92-at-arizona.edu} 12, 22 -- Content-Transfer-Encoding: 7bit 12, 22 -- From: David Elliott {Elliott-at-arizona.edu} 12, 22 -- Subject: Re: [Microscopy] 12, 22 -- Date: Mon, 20 Aug 2007 11:23:35 -0700 12, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 12, 22 -- X-Mailer: Apple Mail (2.752.2) 12, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
I have found that when I ask someone to do something for me that I get more cooperation when I make it easy for them to comply. In your request letter, you might want to include the wording that you would like them to use. In other words, write the acknowledgement for them. You could then suggest that they copy your sentences directly onto their document.
Bob
----- Original Message ----- X-from: {ZZhang-at-uwyo.edu} To: {bob-at-rockisland.com} Sent: Monday, August 20, 2007 10:44 AM
Dear listers, I've spent quite a good deal of time googling for some clues as to how one goes about making a Au on C (also sometimes called gold islands) resolution sample and also an Al/W dendrite sample (which I tried once and didn't seem to get what I was expecting). I couldn't come up with anything about the processes. Does one evaporate or sputter the Au? What type or grade of C is the best substrate? Does one cool the Al/W mixture quickly, slowly, or doesn't it make a difference? I'm going to play with these some, but sometimes it's nice to not have to reinvent the wheel.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
_________________________________________________________________ Need personalized email and website? Look no further. It's easy with Doteasy $0 Web Hosting! Learn more at www.doteasy.com
==============================Original Headers============================== 7, 29 -- From kenconverse-at-qualityimages.biz Mon Aug 20 14:46:44 2007 7, 29 -- Received: from dpmailmta01.doteasy.com (dpmailmta01-30.doteasy.com [65.61.219.10]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7KJkibn023518 7, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 20 Aug 2007 14:46:44 -0500 7, 29 -- Received: from qualityimages.biz (unverified [192.168.101.16]) 7, 29 -- by dpmailmta01.doteasy.com (DEO) with ESMTP id 143958306-1814644 7, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 20 Aug 2007 13:16:59 -0700 7, 29 -- Received: from Ken [72.227.100.25] by qualityimages.biz with ESMTP 7, 29 -- (SMTPD32-8.05) id A10F44770114; Mon, 20 Aug 2007 12:52:47 -0700 7, 29 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 7, 29 -- To: "'MSA Listserver'" {Microscopy-at-MSA.Microscopy.Com} 7, 29 -- Subject: recipe for Au on C and Al/W dendrites 7, 29 -- Date: Mon, 20 Aug 2007 15:52:30 -0400 7, 29 -- Message-ID: {004d01c7e363$a5ad3d50$6401a8c0-at-Ken} 7, 29 -- MIME-Version: 1.0 7, 29 -- Content-Type: text/plain; 7, 29 -- charset="us-ascii" 7, 29 -- Content-Transfer-Encoding: 7bit 7, 29 -- X-Priority: 3 (Normal) 7, 29 -- X-MSMail-Priority: Normal 7, 29 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 7, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 7, 29 -- Thread-Index: AcfjY6RMNcOso0fVQsuA45Otnyrb8w== 7, 29 -- Importance: Normal 7, 29 -- X-IMSTrailer: __IMail_7__ 7, 29 -- X-SpamDetect: ****: 4.100000 SpamUrl=4.1 7, 29 -- X-SpamUrl: doteasy.com 7, 29 -- X-IP-stats: Incoming Last 0, First 16, in=217014, out=0, spam=0 ip=192.168.101.16 7, 29 -- X-Originating-IP: 192.168.101.16 ==============================End of - Headers==============================
Dear Ken, It has been a while since I made these, but I will try to remember. To make the best gold-on-carbon islands, you evaporate pure gold onto polished spectrographic-grade graphite, then post-heat the sample to make the islands coalesce a bit. Takes a few tries to get both the amount of gold and the post heating right. Some of the ones I've seen also have a bit of evaporated tin on top of that; it makes little balls decorating the big balls and is better for a FESEM and high resolution testing. To make the Al-W phase, just put pure Al in a W basket and heat it up until the aluminum melts, then evaporates. Soon after that, the W basket will break at one of the arms, because the Al-W alloy formed is very brittle. The basket with the cooled Al in it will show the dendrites and a pretty Al-W phase. I'm not sure if cooling fast or slow matters, it cools pretty fast when the wire breaks. Good luck,
-----Original Message----- X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] Sent: August 20, 2007 12:54 PM To: maryflet-at-interchange.ubc.ca
Dear listers, I've spent quite a good deal of time googling for some clues as to how one goes about making a Au on C (also sometimes called gold islands) resolution sample and also an Al/W dendrite sample (which I tried once and didn't seem to get what I was expecting). I couldn't come up with anything about the processes. Does one evaporate or sputter the Au? What type or grade of C is the best substrate? Does one cool the Al/W mixture quickly, slowly, or doesn't it make a difference? I'm going to play with these some, but sometimes it's nice to not have to reinvent the wheel.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
_________________________________________________________________ Need personalized email and website? Look no further. It's easy with Doteasy $0 Web Hosting! Learn more at www.doteasy.com
==============================Original Headers============================== 7, 29 -- From kenconverse-at-qualityimages.biz Mon Aug 20 14:46:44 2007 7, 29 -- Received: from dpmailmta01.doteasy.com (dpmailmta01-30.doteasy.com [65.61.219.10]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7KJkibn023518 7, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 20 Aug 2007 14:46:44 -0500 7, 29 -- Received: from qualityimages.biz (unverified [192.168.101.16]) 7, 29 -- by dpmailmta01.doteasy.com (DEO) with ESMTP id 143958306-1814644 7, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 20 Aug 2007 13:16:59 -0700 7, 29 -- Received: from Ken [72.227.100.25] by qualityimages.biz with ESMTP 7, 29 -- (SMTPD32-8.05) id A10F44770114; Mon, 20 Aug 2007 12:52:47 -0700 7, 29 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 7, 29 -- To: "'MSA Listserver'" {Microscopy-at-MSA.Microscopy.Com} 7, 29 -- Subject: recipe for Au on C and Al/W dendrites 7, 29 -- Date: Mon, 20 Aug 2007 15:52:30 -0400 7, 29 -- Message-ID: {004d01c7e363$a5ad3d50$6401a8c0-at-Ken} 7, 29 -- MIME-Version: 1.0 7, 29 -- Content-Type: text/plain; 7, 29 -- charset="us-ascii" 7, 29 -- Content-Transfer-Encoding: 7bit 7, 29 -- X-Priority: 3 (Normal) 7, 29 -- X-MSMail-Priority: Normal 7, 29 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 7, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 7, 29 -- Thread-Index: AcfjY6RMNcOso0fVQsuA45Otnyrb8w== 7, 29 -- Importance: Normal 7, 29 -- X-IMSTrailer: __IMail_7__ 7, 29 -- X-SpamDetect: ****: 4.100000 SpamUrl=4.1 7, 29 -- X-SpamUrl: doteasy.com 7, 29 -- X-IP-stats: Incoming Last 0, First 16, in=217014, out=0, spam=0 ip=192.168.101.16 7, 29 -- X-Originating-IP: 192.168.101.16 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 35 -- From maryflet-at-interchange.ubc.ca Mon Aug 20 15:34:20 2007 16, 35 -- Received: from mr8.mail-relay.ubc.ca (mr8.mail-relay.ubc.ca [137.82.45.15]) 16, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7KKYKBi004346 16, 35 -- for {microscopy-at-microscopy.com} ; Mon, 20 Aug 2007 15:34:20 -0500 16, 35 -- X-Ubc-Received: from mr8.mail-relay.ubc.ca (localhost [127.0.0.1]) 16, 35 -- by localhost (Postfix) with SMTP id BE1EE10D24 16, 35 -- for {microscopy-at-microscopy.com} ; Mon, 20 Aug 2007 13:40:22 -0700 (PDT) 16, 35 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69]) 16, 35 -- by mr8.mail-relay.ubc.ca (Postfix) with ESMTP 16, 35 -- for {microscopy-at-microscopy.com} ; Mon, 20 Aug 2007 13:40:22 -0700 (PDT) 16, 35 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 16, 35 -- by smtp.interchange.ubc.ca 16, 35 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 16, 35 -- with ESMTPS id {0JN3002SAAR9KD-at-smtp.interchange.ubc.ca} for 16, 35 -- microscopy-at-microscopy.com; Mon, 20 Aug 2007 13:40:22 -0700 (PDT) 16, 35 -- Date: Mon, 20 Aug 2007 13:40:15 -0700 16, 35 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 16, 35 -- Subject: RE: [Microscopy] recipe for Au on C and Al/W dendrites 16, 35 -- In-reply-to: {200708201954.l7KJsSfL002176-at-ns.microscopy.com} 16, 35 -- To: kenconverse-at-qualityimages.biz 16, 35 -- Cc: microscopy-at-microscopy.com 16, 35 -- Reply-to: maryflet-at-interchange.ubc.ca 16, 35 -- Message-id: {0JN3002SBAR9KD-at-smtp.interchange.ubc.ca} 16, 35 -- Organization: Materials Eng. 16, 35 -- MIME-version: 1.0 16, 35 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 16, 35 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 16, 35 -- Content-type: text/plain; charset=us-ascii 16, 35 -- Content-transfer-encoding: 7bit 16, 35 -- Thread-index: AcfjZMQfv9U33o4hRNunaQu2ORRJrAAA8t3g 16, 35 -- X-UBC-Scanned: Sophos PureMessage 5.3.2.304607, Antispam-Engine: 2.5.1.298604, Antispam-Data: 2007.8.20.131124 16, 35 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 16, 35 -- X-PerlMx-Spam: Probability=7%, Report=__C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __USER_AGENT_MS_GENERIC 0 16, 35 -- X-Spam-Level: 16, 35 -- X-Spam-Flag: No ==============================End of - Headers==============================
I once saw a beautiful, albeit unintentional, resolution sample of someone who evaporated gold onto an anthracite coal sample. I thought that the gold on carbon were made by evaporating gold onto a warm polished graphite surface. The gold doesn't wet the carbon and forms islands. If you stop the deposition just before coalescence of the Au islands, you have your resolution sample. Gold has a tendency to films by island coalescence on most substrates. That is why it has a rough structure and is not a good choice for high resolution SEM imaging.
For an alloy that forms a dendritic structure, the microstructure will be finer with a higher cooling rate.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: maryflet-at-interchange.ubc.ca [mailto:maryflet-at-interchange.ubc.ca] Sent: Monday, August 20, 2007 1:37 PM To: Walck-at-SouthBayTech.com
Dear Ken, It has been a while since I made these, but I will try to remember. To make the best gold-on-carbon islands, you evaporate pure gold onto polished spectrographic-grade graphite, then post-heat the sample to make the islands coalesce a bit. Takes a few tries to get both the amount of gold and the post heating right. Some of the ones I've seen also have a bit of evaporated tin on top of that; it makes little balls decorating the big balls and is better for a FESEM and high resolution testing. To make the Al-W phase, just put pure Al in a W basket and heat it up until the aluminum melts, then evaporates. Soon after that, the W basket will break at one of the arms, because the Al-W alloy formed is very brittle. The basket with the cooled Al in it will show the dendrites and a pretty Al-W phase. I'm not sure if cooling fast or slow matters, it cools pretty fast when the wire breaks. Good luck,
-----Original Message----- X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] Sent: August 20, 2007 12:54 PM To: maryflet-at-interchange.ubc.ca
Dear listers, I've spent quite a good deal of time googling for some clues as to how one goes about making a Au on C (also sometimes called gold islands) resolution sample and also an Al/W dendrite sample (which I tried once and didn't seem to get what I was expecting). I couldn't come up with anything about the processes. Does one evaporate or sputter the Au? What type or grade of C is the best substrate? Does one cool the Al/W mixture quickly, slowly, or doesn't it make a difference? I'm going to play with these some, but sometimes it's nice to not have to reinvent the wheel.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
_________________________________________________________________ Need personalized email and website? Look no further. It's easy with Doteasy $0 Web Hosting! Learn more at www.doteasy.com
==============================Original Headers============================== 7, 29 -- From kenconverse-at-qualityimages.biz Mon Aug 20 14:46:44 2007 7, 29 -- Received: from dpmailmta01.doteasy.com (dpmailmta01-30.doteasy.com [65.61.219.10]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7KJkibn023518 7, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 20 Aug 2007 14:46:44 -0500 7, 29 -- Received: from qualityimages.biz (unverified [192.168.101.16]) 7, 29 -- by dpmailmta01.doteasy.com (DEO) with ESMTP id 143958306-1814644 7, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 20 Aug 2007 13:16:59 -0700 7, 29 -- Received: from Ken [72.227.100.25] by qualityimages.biz with ESMTP 7, 29 -- (SMTPD32-8.05) id A10F44770114; Mon, 20 Aug 2007 12:52:47 -0700 7, 29 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 7, 29 -- To: "'MSA Listserver'" {Microscopy-at-MSA.Microscopy.Com} 7, 29 -- Subject: recipe for Au on C and Al/W dendrites 7, 29 -- Date: Mon, 20 Aug 2007 15:52:30 -0400 7, 29 -- Message-ID: {004d01c7e363$a5ad3d50$6401a8c0-at-Ken} 7, 29 -- MIME-Version: 1.0 7, 29 -- Content-Type: text/plain; 7, 29 -- charset="us-ascii" 7, 29 -- Content-Transfer-Encoding: 7bit 7, 29 -- X-Priority: 3 (Normal) 7, 29 -- X-MSMail-Priority: Normal 7, 29 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 7, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 7, 29 -- Thread-Index: AcfjY6RMNcOso0fVQsuA45Otnyrb8w== 7, 29 -- Importance: Normal 7, 29 -- X-IMSTrailer: __IMail_7__ 7, 29 -- X-SpamDetect: ****: 4.100000 SpamUrl=4.1 7, 29 -- X-SpamUrl: doteasy.com 7, 29 -- X-IP-stats: Incoming Last 0, First 16, in=217014, out=0, spam=0 ip=192.168.101.16 7, 29 -- X-Originating-IP: 192.168.101.16 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 35 -- From maryflet-at-interchange.ubc.ca Mon Aug 20 15:34:20 2007 16, 35 -- Received: from mr8.mail-relay.ubc.ca (mr8.mail-relay.ubc.ca [137.82.45.15]) 16, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7KKYKBi004346 16, 35 -- for {microscopy-at-microscopy.com} ; Mon, 20 Aug 2007 15:34:20 -0500 16, 35 -- X-Ubc-Received: from mr8.mail-relay.ubc.ca (localhost [127.0.0.1]) 16, 35 -- by localhost (Postfix) with SMTP id BE1EE10D24 16, 35 -- for {microscopy-at-microscopy.com} ; Mon, 20 Aug 2007 13:40:22 -0700 (PDT) 16, 35 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69]) 16, 35 -- by mr8.mail-relay.ubc.ca (Postfix) with ESMTP 16, 35 -- for {microscopy-at-microscopy.com} ; Mon, 20 Aug 2007 13:40:22 -0700 (PDT) 16, 35 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 16, 35 -- by smtp.interchange.ubc.ca 16, 35 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 16, 35 -- with ESMTPS id {0JN3002SAAR9KD-at-smtp.interchange.ubc.ca} for 16, 35 -- microscopy-at-microscopy.com; Mon, 20 Aug 2007 13:40:22 -0700 (PDT) 16, 35 -- Date: Mon, 20 Aug 2007 13:40:15 -0700 16, 35 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 16, 35 -- Subject: RE: [Microscopy] recipe for Au on C and Al/W dendrites 16, 35 -- In-reply-to: {200708201954.l7KJsSfL002176-at-ns.microscopy.com} 16, 35 -- To: kenconverse-at-qualityimages.biz 16, 35 -- Cc: microscopy-at-microscopy.com 16, 35 -- Reply-to: maryflet-at-interchange.ubc.ca 16, 35 -- Message-id: {0JN3002SBAR9KD-at-smtp.interchange.ubc.ca} 16, 35 -- Organization: Materials Eng. 16, 35 -- MIME-version: 1.0 16, 35 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 16, 35 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 16, 35 -- Content-type: text/plain; charset=us-ascii 16, 35 -- Content-transfer-encoding: 7bit 16, 35 -- Thread-index: AcfjZMQfv9U33o4hRNunaQu2ORRJrAAA8t3g 16, 35 -- X-UBC-Scanned: Sophos PureMessage 5.3.2.304607, Antispam-Engine: 2.5.1.298604, Antispam-Data: 2007.8.20.131124 16, 35 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 16, 35 -- X-PerlMx-Spam: Probability=7%, Report=__C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __USER_AGENT_MS_GENERIC 0 16, 35 -- X-Spam-Level: 16, 35 -- X-Spam-Flag: No ==============================End of - Headers==============================
==============================Original Headers============================== 26, 21 -- From walck-at-southbaytech.com Mon Aug 20 15:52:43 2007 26, 21 -- Received: from flpi102.prodigy.net (flpi102.sbcis.sbc.com [207.115.20.71]) 26, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7KKqgr4016639 26, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Aug 2007 15:52:43 -0500 26, 21 -- X-ORBL: [64.169.217.123] 26, 21 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) 26, 21 -- by flpi102.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id l7KKwj9W031099 26, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Aug 2007 13:58:46 -0700 26, 21 -- From: "Scott Walck" {walck-at-southbaytech.com} 26, 21 -- To: {Microscopy-at-microscopy.com} 26, 21 -- Subject: RE: [Microscopy] RE: recipe for Au on C and Al/W dendrites 26, 21 -- Date: Mon, 20 Aug 2007 14:01:33 -0700 26, 21 -- Message-ID: {001101c7e36d$4a12b970$7801a8c0-at-dynamicbl8uno3} 26, 21 -- MIME-Version: 1.0 26, 21 -- Content-Type: text/plain; 26, 21 -- charset="us-ascii" 26, 21 -- Content-Transfer-Encoding: 7bit 26, 21 -- X-Mailer: Microsoft Office Outlook 11 26, 21 -- In-Reply-To: {200708202037.l7KKb091009877-at-ns.microscopy.com} 26, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 26, 21 -- Thread-Index: AcfjarU8jHBpfswiQcOZ+cT/44eqywAAL+Hw ==============================End of - Headers==============================
I would like to thank the following people for letting me know that they are out of their offices and will not be reading my last post to the list serve (until they get back).
Margaret Casey
Richard Doelle
Mario Gislao
Mark Riggs
Wharton Sinkler
Paul VANDERLINDEN
Neil Vincent
Now, let the next round of OoO mail come my way!
David
On Aug 20, 2007, at 11:20 AM, Elliott-at-arizona.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi Zhaojie } } My first thought upon reading your question was "good luck getting } people to acknowledge your facility in their papers", but then I } started thinking. Would it work to send a letter out saying that } unacknowledged work will be retroactively billed to labs at the } 'unsubsidized' (MUCH higher) rate? Just make the case that if they } want subsidies they have to help get them. } } I don't know if it would work as I have not tried it, but I think I } will try it. } } David } } } _____________________ } } David Elliott Ph.D. } Assistant Professor - Department of Cell Biology and Anatomy } Director, Research Microscopy Core Service } University of Arizona College of Medicine } PO Box 245004 } Tucson, AZ 85724 } } Voice: 520-626-7870 } Fax: 520-626-2097 } } } On Aug 20, 2007, at 10:47 AM, ZZhang-at-uwyo.edu wrote: } } } } } } } } } --------------------------------------------------------------------- } } - } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------- } } - } } ------ } } } } Hi everyone: } } } } The Microscopy Core Facility I am directing is heavily funded by a } } couple of NIH institutional grants. As a result, I am able to keep } } most } } of my microscopes updated and people pay less user fees. I need to, } } however, to have the PIs acknowledge these grants in their } } publications. } } } } I keep sending letters to the PIs to request this but without great } } success. I am sure I am not alone and I wonder how other } } people/facilities do this? } } } } Thank you for your help. } } } } } } } } Zhaojie Zhang, Ph.D. } } Director, Microscopy Core Facility } } Department of Zoology and Physiology } } University of Wyoming } } Laramie, WY 82071 } } TEL: 307-766-3038 } } FAX: 307-766-5625 } } zzhang-at-uwyo.edu } } http://www.uwyo.edu/microscopy } } } } } } } } } } } } ==============================Original } } Headers============================== } } 8, 26 -- From ZZhang-at-uwyo.edu Mon Aug 20 12:43:08 2007 } } 8, 26 -- Received: from willowsprings.uwyo.edu } } (willowsprings.uwyo.edu [129.72.10.31]) } } 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id l7KHh8wD018435 } } 8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Aug 2007 } } 12:43:08 -0500 } } 8, 26 -- Received: from UWMAIL.uwyo.edu (uwmail.uwyo.edu } } [172.26.4.76]) } } 8, 26 -- by willowsprings.uwyo.edu (8.13.8/8.13.8) with ESMTP id } } l7KHnAcH021021 } } 8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Aug 2007 } } 11:49:11 -0600 (MDT) } } 8, 26 -- (envelope-from ZZhang-at-uwyo.edu) } } 8, 26 -- Received: from TELEGRAPH5.uwyo.edu ([10.84.60.120]) by } } UWMAIL.uwyo.edu with Microsoft SMTPSVC(6.0.3790.1830); } } 8, 26 -- Mon, 20 Aug 2007 11:47:50 -0600 } } 8, 26 -- x-mimeole: Produced By Microsoft Exchange V6.5 } } 8, 26 -- Content-class: urn:content-classes:message } } 8, 26 -- MIME-Version: 1.0 } } 8, 26 -- Content-Type: text/plain; } } 8, 26 -- charset="US-ASCII" } } 8, 26 -- Subject: } } 8, 26 -- Date: Mon, 20 Aug 2007 11:47:49 -0600 } } 8, 26 -- Message-ID: } } {C9C1AF307F12AF4087DEF87CB0E6A4AD01A3803D-at-TELEGRAPH5.uwyo.edu} } } 8, 26 -- X-MS-Has-Attach: } } 8, 26 -- X-MS-TNEF-Correlator: } } 8, 26 -- Thread-Index: AcfjUjm7eKmZJ3t1SZalWtImdvI4xw== } } 8, 26 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} } } 8, 26 -- To: {Microscopy-at-microscopy.com} } } 8, 26 -- X-OriginalArrivalTime: 20 Aug 2007 17:47:50.0837 (UTC) } } FILETIME=[3A4E1E50:01C7E352] } } 8, 26 -- Content-Transfer-Encoding: 8bit } } 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id l7KHh8wD018435 } } ==============================End of - } } Headers============================== } } } } } ==============================Original } Headers============================== } 12, 22 -- From Elliott-at-arizona.edu Mon Aug 20 13:17:37 2007 } 12, 22 -- Received: from smtpgate.email.arizona.edu } (gandalf.email.Arizona.EDU [128.196.133.169]) } 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l7KIHacr030672 } 12, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 20 Aug 2007 } 13:17:37 -0500 } 12, 22 -- Received: from gandalfs_amavis (amavis3.email.arizona.edu } [10.0.0.206]) } 12, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id } 5A9B1C4EEC } 12, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 20 Aug 2007 } 11:23:39 -0700 (MST) } 12, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) } 12, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id } 9CA27C4882 } 12, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 20 Aug 2007 } 11:23:36 -0700 (MST) } 12, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) } 12, 22 -- In-Reply-To: {200708201747.l7KHlDO2023302-at-ns.microscopy.com} } 12, 22 -- References: {200708201747.l7KHlDO2023302-at-ns.microscopy.com} } 12, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; } format=flowed } 12, 22 -- Message-Id: {DD3A1BDD- } B3DA-40F7-92BD-15F564C9ED92-at-arizona.edu} } 12, 22 -- Content-Transfer-Encoding: 7bit } 12, 22 -- From: David Elliott {Elliott-at-arizona.edu} } 12, 22 -- Subject: Re: [Microscopy] } 12, 22 -- Date: Mon, 20 Aug 2007 11:23:35 -0700 } 12, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} } 12, 22 -- X-Mailer: Apple Mail (2.752.2) } 12, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu } ==============================End of - } Headers============================== }
==============================Original Headers============================== 17, 22 -- From Elliott-at-arizona.edu Mon Aug 20 19:20:49 2007 17, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) 17, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7L0Kmh5024746 17, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 20 Aug 2007 19:20:48 -0500 17, 22 -- Received: from gandalfs_amavis (amavis1.email.arizona.edu [10.0.0.204]) 17, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 44E71D0C8C 17, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 20 Aug 2007 17:26:49 -0700 (MST) 17, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 17, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 682E2C5188 17, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 20 Aug 2007 17:26:48 -0700 (MST) 17, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 17, 22 -- In-Reply-To: {200708201820.l7KIKDe4003149-at-ns.microscopy.com} 17, 22 -- References: {200708201820.l7KIKDe4003149-at-ns.microscopy.com} 17, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 17, 22 -- Message-Id: {01F9AF0C-D68B-4989-A86F-AF4553895073-at-arizona.edu} 17, 22 -- Content-Transfer-Encoding: 7bit 17, 22 -- From: David Elliott {Elliott-at-arizona.edu} 17, 22 -- Subject: Re: OoO 17, 22 -- Date: Mon, 20 Aug 2007 17:26:47 -0700 17, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 17, 22 -- X-Mailer: Apple Mail (2.752.2) 17, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gnf3-at-cdc.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gnf3-at-cdc.gov Name: Maureen Metcalfe
Organization: CDC
Title-Subject: [Filtered] In Situ and Electron Microscopy
Question: Could someone recommend books and/or articles relating to the application of in situ hybridization for electron microscopy?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pwang-at-ues.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: pwang-at-ues.com Name: Ping Wang
Organization: ues
Title-Subject: [Filtered] CCD camera for Hitachi H-600 TEM
Question: Hello!
We have an old Hitachi H-600 TEM and we would like to purchase a CCD camera (new or used) for it.
Please forward the imformation about the compatability of ANY CCD camera (new or used) to Hitachi H-600 TEM.
The Out of Office replies I will get for this posting will take maybe 2 or 3 seconds to delete, but I had to open this message to see what it was about. Frankly I find the complaints about OoO replies much more annoying than the OoOs themselves.
Ralph Common Michigan State University
==============================Original Headers============================== 4, 24 -- From rcommon-at-msu.edu Tue Aug 21 09:32:44 2007 4, 24 -- Received: from sys23.mail.msu.edu (sys23.mail.msu.edu [35.9.75.123]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7LEWg3k002976 4, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Aug 2007 09:32:43 -0500 4, 24 -- Received: from [35.9.122.125] (helo=emlab) 4, 24 -- by sys23.mail.msu.edu with esmtpsa (Exim 4.63 #1) 4, 24 -- (TLSv1:RC4-MD5:128) 4, 24 -- id 1INUsO-0002BN-Mp 4, 24 -- for Microscopy-at-microscopy.com; Tue, 21 Aug 2007 10:38:36 -0400 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: Re: OoO 4, 24 -- Date: Tue, 21 Aug 2007 10:40:00 -0400 4, 24 -- Message-ID: {005301c7e401$28655be0$7d7a0923-at-msu.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="iso-8859-1" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 (Normal) 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1896 4, 24 -- Importance: Normal 4, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
Dear colleagues Registration is now open for a new Workshop on Piezoresponse Force Microscopy, focusing on nanoscale electromechanics in ferroelectrics, polar materials, and biological systems, which will be held at ORNL on October 8-9 in conjunction with the CNMS User Meeting. Registration is available at the ORNL Users Week website http://neutrons.ornl.gov/workshops/users2007/index.shtml. When registering, please (a) choose option $150 (Microscopy + Share) and (b) mark PFM Workshop [checkmark on the bottom ofthe registration form]. Workshop details are available at http://www.cnms.ornl.gov/workshops/CNMS_PFM_Workshop.pdf In addition to tutorials, the workshop will include poster sessions by attendees. A limited number of registration fee wavers and partial compensation of travel expenses will be available for student attendees. Please contact Sergei Kalinin (sergei2-at-ornl.gov) for additional details. Yours Sergei Kalinin and Art Baddorf
==============================Original Headers============================== 1, 20 -- From sergei2-at-ornl.gov Tue Aug 21 17:49:21 2007 1, 20 -- Received: from outbound4.mail.tds.net (outbound4.mail.tds.net [216.170.230.94]) 1, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7LMnL38026193 1, 20 -- for {microscopy-at-microscopy.com} ; Tue, 21 Aug 2007 17:49:21 -0500 1, 20 -- Received: from outaamta02.mail.tds.net (outaamta02.mail.tds.net [216.170.230.32]) 1, 20 -- by outbound4.mail.tds.net (8.13.6/8.13.4) with ESMTP id l7LMnKh9017042 1, 20 -- for {microscopy-at-microscopy.com} ; Tue, 21 Aug 2007 17:49:20 -0500 1, 20 -- Received: from [192.168.0.2] (really [69.130.141.112]) 1, 20 -- by outaamta02.mail.tds.net with ESMTP 1, 20 -- id {20070821224920.YXCS24861.outaamta02.mail.tds.net-at-[192.168.0.2]} 1, 20 -- for {microscopy-at-microscopy.com} ; Tue, 21 Aug 2007 17:49:20 -0500 1, 20 -- Message-ID: {46CB6B4B.5050206-at-ornl.gov} 1, 20 -- Date: Tue, 21 Aug 2007 18:46:35 -0400 1, 20 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 1, 20 -- User-Agent: Thunderbird 1.5.0.12 (Windows/20070509) 1, 20 -- MIME-Version: 1.0 1, 20 -- To: microscopy-at-microscopy.com 1, 20 -- Subject: PFM Workshop - October 8,9 1, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 1, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The free-standing ATF Transformator EA22 110 to 220V step-up transformer required for our Reichert OMU3 ultramicrotome has gone missing and stayed missing sometime in the last year. We know the exact part because we have 2 OMU3 instruments, and only one is now missing the transformer. Does anyone have one they might sell us, or have experience with a serviceable replacement? -- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both atn5613-at-rit.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: atn5613-at-rit.edu Name: Algis Naujokas
Organization: Rochester Institute of Technology
Title-Subject: [Filtered] In need of manual for ion sputtering system IBS TM200S
Question: Hello All, I am a graduate student at Rochester Institute of Technology and am trying get an IBS TM200S sputter device up and running. The problem is we do not have the exact manual for this device. We would be willing to purchase a copy if possible. It would be greatly appreciated as getting this instrument running is key to me starting my research. Please e-mail me off-list. Thank you for your time.
We have a position for a microscopy technician at the UBC BioImaging Facility in Vancouver, British Columbia.
JOB SUMMARY:
To provide assistance with projects performed in the Bio-Imaging facility. Duties include: maintaining laboratory supplies and equipment; maintaining electron and optical microscopes; assisting in developing protocols for new techniques; ordering supplies and equipment; training and performing other related duties.
QUALIFICATIONS:
University degree in Science or equivalent diploma in microscopy (Masters preferred) plus minimum three years microscopy experience. Experience in image processing. Computer experience required (Macintosh and PC). Effective oral and written communication, problem solving, supervisory, interpersonal, organizational skills. Ability to plan and complete work assignments independently. Ability to follow instructions and teach.
I would suggest that you contact Scott Walck at South Bay Technology. You can reach him by email at walck-at-southbaytech.com or at 800-728-2233.
South Bay Technology acquired VCR Group several years ago and has some of the documentation for those older systems. He may be able to come up with a manual for you.
Good luck.
Best regards-
David
David Henriks henriks-at-cox.net
-----Original Message----- X-from: atn5613-at-rit.edu [mailto:atn5613-at-rit.edu] Sent: Wednesday, August 22, 2007 6:55 AM To: Henriks-at-cox.net
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying
please copy both atn5613-at-rit.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: atn5613-at-rit.edu Name: Algis Naujokas
Organization: Rochester Institute of Technology
Title-Subject: [Filtered] In need of manual for ion sputtering system IBS TM200S
Question: Hello All, I am a graduate student at Rochester Institute of Technology and am trying get an IBS TM200S sputter device up and running. The problem is we do not have the exact manual for this device. We would be willing to purchase a copy if possible. It would be greatly appreciated as getting this instrument running is key to me starting my research. Please e-mail me off-list. Thank you for your time.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rnichols-at-bcm.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rnichols-at-bcm.edu Name: Ralph Nichols
Organization: Baylor College of Medicine
Title-Subject: [Filtered] Manual for Coolwell Water Chiller Unit
Question: I have a Coolwell water chiller that chill the water for a Zeiss 902 TEM. It is in need of repair but there is no manual for our facilities engineers to repair it. They have been replacing parts on it that they think will repair it. I try to get manual from Coolwell but they have been out business for a while now. If anyone could send a copy of it,it would be greatly appriciated. The model # is SE 0 80W CZ.
Ralph Nichols Baylor College of Medicine Houston, TX 713 798-5415
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both k.sader-at-leeds.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: k.sader-at-leeds.ac.uk Name: Kasim Sader
Organization: SuperSTEM, UK
Title-Subject: [Filtered] Thin crystals of vermiculite
Question: Dear list,
Does anyone have any thin crystals of vermiculite suitable for electron microscopy that they might be willing to lend? Also, does anyone know which type of vermiculite is the most beam insensitive (or which types are beam sensitive)?
I am looking for a beam insensitive thin crystal and have found a few papers using vermiculite, but if anyone has suggestions of other beam insensitive thin crystals, possibly with larger unit cells, I would appreciate these also.
Thanks,
Kasim Sader Postdoc SuperSTEM Daresbury Laboratories Warrington WA4 4AD UK
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both eq23-at-rice.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: eq23-at-rice.edu Name: Elizabeth
Organization: Rice university
Title-Subject: [Filtered] TEM--any concerns while pregnant?
Question: Greetings,
A graduate student, I'm a frequent user on the Jeol 2010 and 1230 TEMs at the university. I also found out that I'm 2 months pregnant. Should I be concern of any x-ray or other ionizing energy that may leak from the TEMs? Should I wait after the first trimester (a few more weeks) to get back on the TEM? Does anybody have any good information or advice?
I will post soon a summary of all the interesting answers I got for the problem of carbon-free preparation of samples for SEM-EDX (not a lot of solution, though).
Now I face a pretty stupid problem that my pair of neurons can't resolve. When I perform an EDX analysis in SEM (Oxford Instrument's INCA software), I obtain 2 values for each peak: weight % and atom %. I guess that weight % represents the integration of each peak area, whereas atom% represents weight%/atomic weight. Now the 2 values can significantly differ, and thus the element ratio of my samples can also be significantly different. And that is precisely what I want to know!
Now, please allow to ask some questions:
- why is the integration of the peaks in the EDX spectrum called "weight %"? - Does the intensity if the peaks in EDX depend on the atomic weight? In other words, do heavier elements produce more x-rays than lighter elements? It is a hard for me to believe this, because electron shells are the same for light and heavy elements (a K shell is a K shell), however it is the only reason I can see to calculate an atom% value. - Which one of the 2 values to use, in which case and why?
Thank you in advance.
Stephane
____________________________________________________________________________________ Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC
==============================Original Headers============================== 12, 19 -- From nizets2-at-yahoo.com Thu Aug 23 02:22:16 2007 12, 19 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 12, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l7N7MGP2010311 12, 19 -- for {microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 02:22:16 -0500 12, 19 -- Received: (qmail 38583 invoked by uid 60001); 23 Aug 2007 07:22:16 -0000 12, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 19 -- s=s1024; d=yahoo.com; 12, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 12, 19 -- b=56SbosFNfmPiKtfWZKu3HWS47mYAYmsgwcM21fBSvygSpvOjQMM1nuHzUEbAq4Jbunzkcn2TVI8eHOgdpLeI8z4OGEhEfnppqHObYS4VFW1QU8scTot6POOQEPMGa1V50I7hrM1CLvDvIIHR7xA5QJgZ4GM/AWnM1OxG5rca/Q8=; 12, 19 -- X-YMail-OSG: 07SBxI4VM1nZ7Fk0PON.Y1khqer81hRqg041bYB6G4cwRrmmtCHzE336KWfbXPpfhvZMkGL7oRyx02fjI.jE0RgTLcEZNUjuIMwnyuvsq0jY_hzdiM2XjMpX.w5hGw-- 12, 19 -- Received: from [80.122.101.102] by web37405.mail.mud.yahoo.com via HTTP; Thu, 23 Aug 2007 00:22:15 PDT 12, 19 -- Date: Thu, 23 Aug 2007 00:22:15 -0700 (PDT) 12, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 12, 19 -- Subject: SEM: wt% or atom% ? 12, 19 -- To: microscopy-at-microscopy.com 12, 19 -- MIME-Version: 1.0 12, 19 -- Content-Type: text/plain; charset=iso-8859-1 12, 19 -- Content-Transfer-Encoding: 8bit 12, 19 -- Message-ID: {963133.38423.qm-at-web37405.mail.mud.yahoo.com} ==============================End of - Headers==============================
Dear listers, Here are the replies I got. Very helpful. Thank you all. Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
I inadvertently made a Al/W dendrite sample by heating Al foil (or wire, I don't remember) in a W coil basket in my vacuum evaporator when making a telescope mirror. The Al embrittles the W and as you evaporate the Al, eventually the W wire breaks and you are left with a glob of Al in the basket. Looks great in BSE mode. Henk **************************************************************************** ***
I have made the Al-W dendritic structures in an evaporator. From my observations; 1. Use excess Al for the charge. 2. Heat the charge until the Al is molten. I usually cut-back on heating power, you need just enough to keep the Al molten for about 3 minutes, without evaporating it all away. 3. Cool the molten blob rapidly by just shutting off the heater power. 4. The dendritic structures are located in small "pockets" near the top of the now solidified blob. Joseph M. Oparowski **************************************************************************** *****
I don't know the answer to your question but as far as dendritic grow is concerned, here is what I do know...
Dendritic growth is a process where one constituent of an alloy begins to solidfy before another. This leaves the liquid with a compostion lower in the first constituent and higher in the second. The process of the growing is limited by a diffusion process of the two components in the liquid. This means you get smaller dendrites with faster cooling rates and larger dendrites with slower cooling rates. However, if you cool fast enough, you can't get the diffusion process happening, and so then you don't get any dendritic growth. If you cool really slow, well, then it depends upon the alloy....
You have asked about Al/W. Now those two have really different melting points so I'd think you'd get darned good dentritic growth with those two just about no matter how you cooled it... If you went slow, I'd bet you'd get a lot of interdendritic shrinkage cavities though....
Also, I don't know how big of a structure you want, but it would be my guess that you could cool that alloy pretty quick and have some nice dendritic growth...
What did you do the first time you tried that? And how did it turn out?
If someone does answer you with a lot more info, I'd like to find out myself...If you don't mind sending along the info..
Dj ***************************************************************************
It has been a while since I made these, but I will try to remember. To make the best gold-on-carbon islands, you evaporate pure gold onto polished spectrographic-grade graphite, then post-heat the sample to make the islands coalesce a bit. Takes a few tries to get both the amount of gold and the post heating right. Some of the ones I've seen also have a bit of evaporated tin on top of that; it makes little balls decorating the big balls and is better for a FESEM and high resolution testing. To make the Al-W phase, just put pure Al in a W basket and heat it up until the aluminum melts, then evaporates. Soon after that, the W basket will break at one of the arms, because the Al-W alloy formed is very brittle. The basket with the cooled Al in it will show the dendrites and a pretty Al-W phase. I'm not sure if cooling fast or slow matters, it cools pretty fast when the wire breaks. Good luck,
Mary Fletcher **************************************************************************** **
I once saw a beautiful, albeit unintentional, resolution sample of someone who evaporated gold onto an anthracite coal sample. I thought that the gold on carbon were made by evaporating gold onto a warm polished graphite surface. The gold doesn't wet the carbon and forms islands. If you stop the deposition just before coalescence of the Au islands, you have your resolution sample. Gold has a tendency to films by island coalescence on most substrates. That is why it has a rough structure and is not a good choice for high resolution SEM imaging.
For an alloy that forms a dendritic structure, the microstructure will be finer with a higher cooling rate.
Scott D. Walck, Ph.D. **************************************************************************** ****
_________________________________________________________________ Need personalized email and website? Look no further. It's easy with Doteasy $0 Web Hosting! Learn more at www.doteasy.com
==============================Original Headers============================== 21, 30 -- From kenconverse-at-qualityimages.biz Thu Aug 23 05:57:45 2007 21, 30 -- Received: from dpmailmta01.doteasy.com (dpmailmta01-02.doteasy.com [65.61.218.2]) 21, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7NAviF3027802 21, 30 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 23 Aug 2007 05:57:44 -0500 21, 30 -- Received: from qualityimages.biz (unverified [192.168.101.16]) 21, 30 -- by dpmailmta01.doteasy.com (DEO) with ESMTP id 144594340-1814644 21, 30 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 23 Aug 2007 04:22:06 -0700 21, 30 -- Received: from Ken [72.227.100.25] by qualityimages.biz with ESMTP 21, 30 -- (SMTPD32-8.05) id A7C66C5800F8; Thu, 23 Aug 2007 03:56:06 -0700 21, 30 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 21, 30 -- To: "'MSA Listserver'" {Microscopy-at-MSA.Microscopy.Com} 21, 30 -- Subject: Re: [Microscopy] recipe for Au on C and Al/W dendrites 21, 30 -- Date: Thu, 23 Aug 2007 06:57:25 -0400 21, 30 -- Message-ID: {000e01c7e574$6513ec40$6401a8c0-at-Ken} 21, 30 -- MIME-Version: 1.0 21, 30 -- Content-Type: text/plain; 21, 30 -- charset="us-ascii" 21, 30 -- X-Priority: 3 (Normal) 21, 30 -- X-MSMail-Priority: Normal 21, 30 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 21, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 21, 30 -- Thread-Index: AcfldGN3YmW81roMQxCHjXx8Wj9zSQ== 21, 30 -- Importance: Normal 21, 30 -- X-IMSTrailer: __IMail_7__ 21, 30 -- X-SpamDetect: ****: 4.100000 SpamUrl=4.1 21, 30 -- X-SpamUrl: doteasy.com 21, 30 -- X-IP-stats: Incoming Last 0, First 19, in=255648, out=0, spam=0 ip=192.168.101.16 21, 30 -- X-Originating-IP: 192.168.101.16 21, 30 -- Content-Transfer-Encoding: 8bit 21, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7NAviF3027802 ==============================End of - Headers==============================
Being new in geology environment, can someone enlighten me on how can I differentiate calcite, aragonite and dolomite using BSE? Reference to a title/name of a publication is also welcome.
Thanks,
Melina Miralles The Petroleum Institute Abu Dhabi, UAE PGSc Lab Technician The Petroleum Institute Abu Dhabi, UAE
==============================Original Headers============================== 6, 27 -- From mmiralles-at-pi.ac.ae Thu Aug 23 06:12:31 2007 6, 27 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7NBCUVw007311 6, 27 -- for {microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 06:12:31 -0500 6, 27 -- Received: from unknown (HELO pi-exf2.PI.AC.AE) ([192.168.2.13]) 6, 27 -- by mx1.pi.ac.ae with ESMTP; 23 Aug 2007 15:19:10 +0400 6, 27 -- X-IronPort-AV: i="4.19,300,1183320000"; 6, 27 -- d="scan'208"; a="1905270:sNHT25977920" 6, 27 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by pi-exf2.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.1830); 6, 27 -- Thu, 23 Aug 2007 15:12:29 +0400 6, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 27 -- Content-class: urn:content-classes:message 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- charset="US-ASCII" 6, 27 -- Subject: Differentiating CaCO3 using BSE 6, 27 -- Date: Thu, 23 Aug 2007 15:12:29 +0400 6, 27 -- Message-ID: {D5603421C6303A46883F87E7968F285B031AE6C9-at-pi-exm.PI.AC.AE} 6, 27 -- X-MS-Has-Attach: 6, 27 -- X-MS-TNEF-Correlator: 6, 27 -- Thread-Topic: Differentiating CaCO3 using BSE 6, 27 -- Thread-Index: Acfldn4/yG4ZQcLrRTauzL9bsnrSFw== 6, 27 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae} 6, 27 -- To: {microscopy-at-microscopy.com} 6, 27 -- X-OriginalArrivalTime: 23 Aug 2007 11:12:29.0787 (UTC) FILETIME=[7EB262B0:01C7E576] 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7NBCUVw007311 ==============================End of - Headers==============================
I have been requested to look at a medical item surgically removed from a (living) person and need to determine whether or not to accept this job. Being a materials person, I am quite unexperienced at biological issues. What are some considerations to be aware of to help me decide if this job is appropriate for us or not? I don't know how (or if) it has been cleaned so far, but I've been told that I won't be allowed to do anything to it other than examine it. Sorry, but at this time I can not be more specific.
I have already gotten some valuable and much appreciated input from one local member, but thought I would pose this to the larger community as well.
As always, TIA.
Chris Holp FirstEnergy Corp. BETA Labs Mayfield Village, OH 44143 440-604-9704 holpc-at-firstenergycorp.com
----------------------------------------- The information contained in this message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately, and delete the original message.
==============================Original Headers============================== 8, 20 -- From holpc-at-firstenergycorp.com Thu Aug 23 08:11:57 2007 8, 20 -- Received: from firstenergycorp.com (gw19.firstenergycorp.com [205.132.74.180]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7NDBuG2021705 8, 20 -- for {microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 08:11:56 -0500 8, 20 -- Subject: SEM, considerations for medical devices 8, 20 -- To: Microscopy-at-microscopy.com 8, 20 -- X-Mailer: Lotus Notes Release 6.5.4 CCH5 September 12, 2005 8, 20 -- Message-ID: {OF8779EC7F.9CB80750-ON85257340.0045A307-85257340.004878F0-at-FirstEnergyCorp.com} 8, 20 -- From: holpc-at-firstenergycorp.com 8, 20 -- Date: Thu, 23 Aug 2007 09:11:35 -0400 8, 20 -- MIME-Version: 1.0 8, 20 -- X-MIMETrack: Serialize by Router on mail01/Servers/FirstEnergy(Release 7.0.2FP2|May 14, 2007) at 8, 20 -- 08/23/2007 09:11:36, 8, 20 -- Itemize by SMTP Server on GW13/Servers/FirstEnergy(Release 7.0.2FP1|January 8, 20 -- 10, 2007) at 08/23/2007 09:11:36 AM, 8, 20 -- Serialize by Router on GW13/Servers/FirstEnergy(Release 7.0.2FP1|January 10, 2007) at 8, 20 -- 08/23/2007 09:11:38 AM, 8, 20 -- Serialize complete at 08/23/2007 09:11:38 AM 8, 20 -- Content-Type: text/plain; 8, 20 -- charset="US-ASCII" ==============================End of - Headers==============================
I don't think one can reliably distinguish calcite and aragonite using BSE alone unless their morphologies are diagnostic. On the other hand, distinguishing dolomite should be possible because it has about 20% MgO.
The mean atomic numbers for calcite and aragonite are the same, and both have a backscatter coefficient of about 0.142. Dolomite, though, is less dense and has a backscatter coefficient of about 0.124, so it should appear darker than calcite and aragonite.
This does not mean that there will be absolutely no contrast difference between calcite and aragonite -- it simply will not be clear which mineral is which, though. Both minerals can have various impurities (Mn, Mg, Fe, etc) and crystal orientations that will add variation to the backscatter signal.
As I mentioned, you might have to rely on their morphologies if you're working only with BSE. The crystal lattice of aragonite is different than that of calcite, resulting in different shapes. Aragonite has an orthorhombic system with usually needle-like crystals. Calcite is trigonal-rhombohedral and has a variety of habits: fibrous, granular, lamellar, etc -- more than are easily described here. You can either use the web or consult an introductory textbook on mineralogy or petrography to view examples of the different morphologies of calcite and aragonite.
If the morphologies in your samples don't lend themselves to clearly identifying calcite vs aragonite, you'll have to something like XRD or EBSD to differentiate the crystal lattices.
Best, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu Personal Website: http://umn.edu/~frah0010
On Aug 23, 2007, at 6:16 AM, mmiralles-at-pi.ac.ae wrote:
} Hi everyone, } } Being new in geology environment, can someone enlighten me on how } can I } differentiate calcite, aragonite and dolomite using BSE? } Reference to a title/name of a publication is also welcome. } } Thanks, } } Melina Miralles } The Petroleum Institute } Abu Dhabi, UAE } PGSc Lab Technician } The Petroleum Institute } Abu Dhabi, UAE
==============================Original Headers============================== 13, 19 -- From frah0010-at-umn.edu Thu Aug 23 08:22:37 2007 13, 19 -- Received: from mta-m2.tc.umn.edu (mta-m2.tc.umn.edu [134.84.119.106]) 13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7NDMbbR001185 13, 19 -- for {microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 08:22:37 -0500 13, 19 -- Received: from [160.94.146.133] (212-swing.geo.umn.edu [160.94.146.133]) 13, 19 -- by mta-m2.tc.umn.edu (UMN smtpd) with ESMTP 13, 19 -- Thu, 23 Aug 2007 08:22:33 -0500 (CDT) 13, 19 -- X-Umn-Remote-Mta: [N] 212-swing.geo.umn.edu [160.94.146.133] #+LO+TS+AU+HN 13, 19 -- Mime-Version: 1.0 (Apple Message framework v752.2) 13, 19 -- In-Reply-To: {200708231116.l7NBGb4V016205-at-ns.microscopy.com} 13, 19 -- References: {200708231116.l7NBGb4V016205-at-ns.microscopy.com} 13, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 13, 19 -- Message-Id: {4201AB2B-A9FC-455F-98A2-AF735EE06F69-at-umn.edu} 13, 19 -- Content-Transfer-Encoding: 7bit 13, 19 -- From: Ellery Frahm {frah0010-at-umn.edu} 13, 19 -- Subject: Re: [Microscopy] Differentiating CaCO3 using BSE 13, 19 -- Date: Thu, 23 Aug 2007 08:22:30 -0500 13, 19 -- To: mmiralles-at-pi.ac.ae, microscopy-at-microscopy.com 13, 19 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
The first thing I'd do is go to http://www.histonet.org and sign up on the histonet mailing list and post your question. That list is full of people who do this sort of work clinically and in research labs. Including authors of some of the standard histotechnique texts.
Second, check with the people who want to send you the sample and get the details of what they've done and what you can do. At the very least, make certain there was no infection associated with the device or that there is otherwise zero chance of any pathogen coming along with it. Since you're in a materials lab, you will not have any provisions with dealing with any live bacteria/fungi, etc. You should get the sample either fixed, cleaned of biological materials, or if neither, then guaranteed to be healthy. What sorts of microscopy are they looking for? "... won't be allowed to do anything to it other than examine it." really limits what you can do.
Phil
} Good morning everyone, } } I have been requested to look at a medical item surgically removed from a } (living) person and need to determine whether or not to accept this job. } Being a materials person, I am quite unexperienced at biological issues. } What are some considerations to be aware of to help me decide if this job } is appropriate for us or not? I don't know how (or if) it has been cleaned } so far, but I've been told that I won't be allowed to do anything to it } other than examine it. Sorry, but at this time I can not be more specific. } } I have already gotten some valuable and much appreciated input from one } local member, but thought I would pose this to the larger community as } well. } } As always, TIA. } } Chris Holp } FirstEnergy Corp. } BETA Labs } Mayfield Village, OH 44143 } 440-604-9704 } holpc-at-firstenergycorp.com -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859
==============================Original Headers============================== 5, 22 -- From oshel1pe-at-cmich.edu Thu Aug 23 08:31:30 2007 5, 22 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7NDVU0i013097 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 08:31:30 -0500 5, 22 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 22 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id l7NDrYYA023248 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 09:53:42 -0400 5, 22 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 5, 22 -- Thu, 23 Aug 2007 09:31:26 -0400 5, 22 -- Mime-Version: 1.0 5, 22 -- Message-Id: {f06240804c2f33b4fb49b-at-[141.209.160.249]} 5, 22 -- In-Reply-To: {200708231316.l7NDGkPq028482-at-ns.microscopy.com} 5, 22 -- References: {200708231316.l7NDGkPq028482-at-ns.microscopy.com} 5, 22 -- Date: Thu, 23 Aug 2007 09:31:25 -0400 5, 22 -- To: Microscopy-at-microscopy.com 5, 22 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 22 -- Subject: Re: [Microscopy] SEM, considerations for medical devices 5, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 22 -- X-OriginalArrivalTime: 23 Aug 2007 13:31:26.0913 (UTC) FILETIME=[E802DB10:01C7E589] 5, 22 -- X-CanItPRO-Stream: default 5, 22 -- X-Spam-Score: -4 () L_EXCH_MF 5, 22 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
Let's assume that you're being asked to look at is either a medical device like a pacemaker or an electrical lead from one or an implant like an artificial joint or tooth filling -- I won't ask you to confirm or deny that -- but that's what I'm assuming for this comment.
We've analyzed such items before (although usually before implantation). Hospitals have strict procedures for anything that doesn't go into the medical waste, usually involving autoclave sterilization. If you are worried about biological hazards or anything like that, you should feel absolutely free to ask the company exactly what has been done to clean it. If it shows up in your laboratory, though, in a biohazard bag, feel free to send it back to them and say "No way." But since medical devices are made to be sterilized before being placed in a human body, they can be cleaned very well, probably cleaner than most other samples.
In the past, we've been assured of cleanliness, and I handle most samples with medical gloves anyway because there are plenty of toxic geological or mat sci samples too. Basically, medical devices can be sterilized -- ask them to do that or find someone else.
Best, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu Personal Website: http://umn.edu/~frah0010
On Aug 23, 2007, at 8:17 AM, holpc-at-firstenergycorp.com wrote:
} Good morning everyone, } } I have been requested to look at a medical item surgically removed } from a } (living) person and need to determine whether or not to accept this } job. } Being a materials person, I am quite unexperienced at biological } issues. } What are some considerations to be aware of to help me decide if } this job } is appropriate for us or not? I don't know how (or if) it has been } cleaned } so far, but I've been told that I won't be allowed to do anything } to it } other than examine it. Sorry, but at this time I can not be more } specific. } } I have already gotten some valuable and much appreciated input from } one } local member, but thought I would pose this to the larger community as } well. } } As always, TIA. } } Chris Holp } FirstEnergy Corp. } BETA Labs } Mayfield Village, OH 44143 } 440-604-9704 } holpc-at-firstenergycorp.com
==============================Original Headers============================== 10, 19 -- From frah0010-at-umn.edu Thu Aug 23 08:42:45 2007 10, 19 -- Received: from mta-m2.tc.umn.edu (mta-m2.tc.umn.edu [134.84.119.106]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7NDgjRG024980 10, 19 -- for {microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 08:42:45 -0500 10, 19 -- Received: from [160.94.146.133] (212-swing.geo.umn.edu [160.94.146.133]) 10, 19 -- by mta-m2.tc.umn.edu (UMN smtpd) with ESMTP 10, 19 -- Thu, 23 Aug 2007 08:42:45 -0500 (CDT) 10, 19 -- X-Umn-Remote-Mta: [N] 212-swing.geo.umn.edu [160.94.146.133] #+LO+TS+AU+HN 10, 19 -- Mime-Version: 1.0 (Apple Message framework v752.2) 10, 19 -- In-Reply-To: {200708231317.l7NDHwci030709-at-ns.microscopy.com} 10, 19 -- References: {200708231317.l7NDHwci030709-at-ns.microscopy.com} 10, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 10, 19 -- Message-Id: {7D11DC9D-D1DD-400A-BBA3-5A435DF81B05-at-umn.edu} 10, 19 -- Content-Transfer-Encoding: 7bit 10, 19 -- From: Ellery Frahm {frah0010-at-umn.edu} 10, 19 -- Subject: Re: [Microscopy] SEM, considerations for medical devices 10, 19 -- Date: Thu, 23 Aug 2007 08:42:42 -0500 10, 19 -- To: holpc-at-firstenergycorp.com, microscopy-at-microscopy.com 10, 19 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
The principal axiom of "universal precautions", required for handling human biological material, is to assume that ALL samples are potentially infectious and treat them as such. Unless you can positively confirm that the objects have been sterilized or fixed, they should be handled as if they are infectious, and not accept any assurances that there is no infection involved.
Ralph Common Division of Human Pathology Michigan State University
==============================Original Headers============================== 4, 24 -- From rcommon-at-msu.edu Thu Aug 23 09:03:04 2007 4, 24 -- Received: from sys15.mail.msu.edu (sys15.mail.msu.edu [35.9.75.115]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7NE34sr004730 4, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 09:03:04 -0500 4, 24 -- Received: from [35.9.122.125] (helo=emlab) 4, 24 -- by sys15.mail.msu.edu with esmtpsa (Exim 4.63 #1) 4, 24 -- (TLSv1:RC4-MD5:128) 4, 24 -- id 1IODH5-0007gM-SF 4, 24 -- for Microscopy-at-microscopy.com; Thu, 23 Aug 2007 10:03:03 -0400 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: SEM, considerations for medical devices 4, 24 -- Date: Thu, 23 Aug 2007 10:04:27 -0400 4, 24 -- Message-ID: {007401c7e58e$85852240$7d7a0923-at-msu.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="iso-8859-1" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 (Normal) 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1896 4, 24 -- Importance: Normal 4, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
Let me address your questions (as I understand them):
} 1: why is the integration of the peaks in the EDX } spectrum called "weight %"?
We have the problem of two uses of the term "weight percent" in X-ray analysis. There is the use of "weight" to describe the relative intensities of the X-ray lines themselves -- that is, the relative intensities of, say, L-alpha and L-beta X-rays -- and this value can be reported as a "weight percent" or coefficient. There is also the use of "weight percent" to refer to the resulting data, calculated in terms of the mass fraction of the elements in a sample. These two uses are creating confusion here. It sounds to me like the software is integrating the area of the peaks to calculate your element concentrations which are being reported in terms of "weight percent" (mass fraction) and "atomic percent" (atom fraction) -- does that make sense with what you're seeing?
} 2; Does the intensity if the peaks in EDX depend on the } atomic weight? In other words, do heavier elements } produce more x-rays than lighter elements?
It is not that simple, and the accelerating voltage how efficiently X- rays are produced from different elements (see "overvoltage ratio"), so it differs at, say, 10 kV and 20 kV. The simple answer is no. The not-so-simple answer involves a lot more than I am willing to type at the moment -- try consulting the section on characteristic X- ray production in Goldstein et al.
} 3; Which one of the 2 values to use, in which case and why?
If you are asking about asking about reporting your results in terms of atomic fraction or mass fraction, that depends on your application or research question and the "standard" format for your field. Why not record or report both? You can also convert data from one form to the other at a later date using Excel and a periodic table. So the answer is "it depends."
I apologize if I've misunderstood any of your questions or problem descriptions.
Best, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu Personal Website: http://umn.edu/~frah0010
==============================Original Headers============================== 11, 19 -- From frah0010-at-umn.edu Thu Aug 23 09:09:03 2007 11, 19 -- Received: from mta-m2.tc.umn.edu (mta-m2.tc.umn.edu [134.84.119.106]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7NE93Ev011289 11, 19 -- for {microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 09:09:03 -0500 11, 19 -- Received: from [160.94.146.133] (212-swing.geo.umn.edu [160.94.146.133]) 11, 19 -- by mta-m2.tc.umn.edu (UMN smtpd) with ESMTP 11, 19 -- Thu, 23 Aug 2007 09:09:03 -0500 (CDT) 11, 19 -- X-Umn-Remote-Mta: [N] 212-swing.geo.umn.edu [160.94.146.133] #+LO+TS+AU+HN 11, 19 -- Mime-Version: 1.0 (Apple Message framework v752.2) 11, 19 -- In-Reply-To: {200708230728.l7N7S5T1019139-at-ns.microscopy.com} 11, 19 -- References: {200708230728.l7N7S5T1019139-at-ns.microscopy.com} 11, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 11, 19 -- Message-Id: {461AB14B-0E89-474B-A89B-C783D0A66855-at-umn.edu} 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- From: Ellery Frahm {frah0010-at-umn.edu} 11, 19 -- Subject: Re: [Microscopy] SEM: wt% or atom% ? 11, 19 -- Date: Thu, 23 Aug 2007 09:09:00 -0500 11, 19 -- To: nizets2-at-yahoo.com, microscopy-at-microscopy.com 11, 19 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
Has anyone on the list had experience using H.S.Q. (hydrogen silsesquioxane) as a negative e-beam resist? If so has it been useful, any tips you can share as to ease of use, a good source for the material. Thanks for any help you can offer.
David
M. David Frey Senior Application Engineer Rensselaer Polytechnic Institute Low Center for Industrial Inn. Center for Integrated Electronics 110 8th Street CII 4161 (Office) CII 6015 (Packages and Mail) Troy, NY 12180 518-276-3323 (office) 518-698-2288 (mobile)
==============================Original Headers============================== 7, 23 -- From freym2-at-rpi.edu Thu Aug 23 09:22:54 2007 7, 23 -- Received: from smtp6.server.rpi.edu (smtp6.server.rpi.edu [128.113.2.226]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7NEMsrU028575 7, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 09:22:54 -0500 7, 23 -- Received: from Frey (sponge-bob-49.dynamic.rpi.edu [128.113.222.88]) 7, 23 -- by smtp6.server.rpi.edu (8.13.1/8.13.1) with ESMTP id l7NEMooW032158 7, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 10:22:54 -0400 7, 23 -- From: "M. David Frey" {freym2-at-rpi.edu} 7, 23 -- To: {Microscopy-at-microscopy.com} 7, 23 -- Subject: e-Beam lithography Negative Resists 7, 23 -- Date: Thu, 23 Aug 2007 10:22:45 -0400 7, 23 -- Message-ID: {002601c7e591$132b1e40$58de7180-at-Frey} 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="US-ASCII" 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- X-Mailer: Microsoft Office Outlook 11 7, 23 -- Thread-Index: AcflkRLVJ97W8rjmQA2TlxYRx1aARg== 7, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 7, 23 -- X-RPI-SA-Score: undef - spam scanning disabled 7, 23 -- X-CanItPRO-Stream: default 7, 23 -- X-Canit-Stats-ID: Bayes signature not available 7, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 128.113.2.226 ==============================End of - Headers==============================
We have an Oxford system here so I think I can comment on this with authority.
The weight% and atom% labels DEFINITELY refer to the two ways of expressing results.
Weight% is synonymous with mass%. It is only roughly proportional to peak intensity. As Ellery pointed out, there are many things that affect the conversion of raw peak intensity to weight fraction. The only time I make use of the correlation is if I am estimating compositions by eye. A 1 wt% S Ka peak and a 1 wt% Au Ma peak have _roughly_ the same integral where the atomic fractions would be widely different.
Atomic% is synonymous with mole%. It is directly related to weight% by the atomic weight of the species. It can be easily calculated using a spreadsheet; however, every (computerized) EDS system I have seen offers atomic% as an output option.
The two numbers are often widely different, so which do you use? It depends. If you are interested in stoichiometry and formulas, you want atomic fractions. If you are checking a sample against its formulation, the sample was undoubtedly weighed out and you likely want mass fraction. For example, I was working on a sample of EuAl2 for a researcher yesterday. The mole (or atomic) fraction of Eu is 33.3%. However, the mass fraction is 73.8%. Both answers are "right".
Regarding your question of why does intensity depend on weight fraction more than atomic fraction, I would have to dig back into the texts to say for sure. Weight depends (essentially) on the mass of the nucleus and that tells you the number of protons and neutrons and thus the number of electrons. Now whether it is the number of electrons or the mass of the nucleus that determines the x-ray intensity from an atom, I would have to go look. The answer could be a combination of factors.
Warren
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, August 23, 2007 2:23 AM To: wesaia-at-iastate.edu
Hello all,
We have an Hitachi S4500, vintage ~1993. We recently added a digital acquisition & EDX system. The acquisition system sits by idle while you tune up the image (select area, magnification, focus, etc.) on the scope's video monitors, then takes over to acquire the image. Everything works beautifully, but the ergonomics are not very good. We have our PC monitors sitting on top of the console, so they are uncomfortably high to look at.
I would like to simply remove the console CRTs and replace either with a small monitor, or even better, pipe the video signal into an image capture board to display in a window on the acquisition PC. That's where my question lies--how to pull the video signal out. I realize the console has a video out BNC connector which I have tried, but the quality isn't quite there. (Maybe a sync problem? the image is somewhat distorted.) Our electronics guys say the old CRTs are hardwired into the system. Does anyone know if this is a standard video signal? If so, we'll simply remove the old CRTs (replacing the hard-wired connections with a standard connection) and cut down the console, leaving a place for the PC monitors. If there is no standard video signal, then things get substantially more complicated, of course.
Any suggestions or insight would be appreciated.
Jim
---------------------------------------------- Jim Passmore Research Associate Sealed Air Corporation james.passmore-at-sealedair.com 864-433-2927 voice 864-433-2205 fax ----------------------------------------------
==============================Original Headers============================== 8, 15 -- From James.Passmore-at-sealedair.com Thu Aug 23 11:00:14 2007 8, 15 -- Received: from dunnotesgw01.sealedair.com (dunnotesgw01.sealedair.com [165.225.194.17]) 8, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7NG0DBs029849 8, 15 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 11:00:13 -0500 8, 15 -- Subject: SEM -- S4500 video monitors 8, 15 -- Sensitivity: 8, 15 -- To: Microscopy-at-microscopy.com 8, 15 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 8, 15 -- Message-ID: {OF67EFDB4C.4B39C2AB-ON8525733B.00684FC2-85257340.0057E12A-at-sealedair.com} 8, 15 -- From: James.Passmore-at-sealedair.com 8, 15 -- Date: Thu, 23 Aug 2007 11:59:52 -0400 8, 15 -- X-MIMETrack: Serialize by Router on DUNNOTESGW01/SAC(Release 6.5.4FP1 | June 19, 2005) at 8, 15 -- 08/23/2007 04:00:11 PM 8, 15 -- MIME-Version: 1.0 8, 15 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
probably just wishful thinking but... Has anyone had any experience with getting a PC to interface to the antiquated Tracor Northern PAC stage/ spectro controller???
Regards,
B. Vanden Berg
==============================Original Headers============================== 4, 29 -- From BVandenberg-at-inco.com Thu Aug 23 11:02:33 2007 4, 29 -- Received: from IronPort-TOR2.inco.com (smtp.tor.inco.com [142.47.133.70]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7NG2XRC000738 4, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 11:02:33 -0500 4, 29 -- X-IronPort-AV: E=Sophos;i="4.19,300,1183348800"; 4, 29 -- d="scan'208";a="30716982" 4, 29 -- Received: from unknown (HELO tco-msxfe2.INCO.NET) ([142.40.254.16]) 4, 29 -- by IronPort-TOR2.inco.com with ESMTP; 23 Aug 2007 12:06:20 -0400 4, 29 -- Received: from SUD-MSX06.INCO.NET ([142.40.5.168]) by tco-msxfe2.INCO.NET with Microsoft SMTPSVC(6.0.3790.1830); 4, 29 -- Thu, 23 Aug 2007 12:00:36 -0400 4, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 4, 29 -- Content-class: urn:content-classes:message 4, 29 -- MIME-Version: 1.0 4, 29 -- Content-Type: text/plain; 4, 29 -- charset="us-ascii" 4, 29 -- Subject: Tracor PAC 5600 stage control 4, 29 -- Date: Thu, 23 Aug 2007 12:00:34 -0400 4, 29 -- Message-ID: {45286943FB4B6F4A87B56FB295A7AD5E01701494-at-SUD-MSX06.INCO.NET} 4, 29 -- X-MS-Has-Attach: 4, 29 -- X-MS-TNEF-Correlator: 4, 29 -- Thread-Topic: Tracor PAC 5600 stage control 4, 29 -- Thread-Index: Acflnr1G7a8mgOiVQiCbmRmovzkzjQ== 4, 29 -- From: "Vandenberg, Ben \(Sudbury\)" {BVandenberg-at-inco.com} 4, 29 -- To: {Microscopy-at-microscopy.com} 4, 29 -- X-OriginalArrivalTime: 23 Aug 2007 16:00:36.0351 (UTC) FILETIME=[BE4AB8F0:01C7E59E] 4, 29 -- X-TM-AS-Product-Ver: SMEX-7.0.0.1557-5.0.1021-15380.000 4, 29 -- X-TM-AS-Result: No--3.993500-8.000000-31 4, 29 -- Content-Transfer-Encoding: 8bit 4, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7NG2XRC000738 ==============================End of - Headers==============================
Dear Elizabeth, I have had various graduate students and technicians working in my lab while pregnant and on my 200 kV Hitachi TEM. The instrument was checked by the university's Radiation Control Officer with a Geiger counter when it was first installed and periodically after, particularly after the gun was disassembled and serviced. The pregnant ladies also wore film badges, which are much more sensitive to accumulated radiation than a Geiger counter. No significant leakage was ever found, in fact, the bricks in the walls turned out to emit more background radiation than the TEM operating at 200kV. Having said that, you can check that the TEM is operating within radiation emission limits by: 1. Having it checked while it is operating normally, with you in the position where you normally operate the TEM 2. Always make sure all apertures, particularly the moveable condenser aperture, are in place before turning on the beam. Let someone else align the beam with the C aperture out, if necessary. I think the modern microscopes are very well shielded and do not leak significant radiation. Regards,
-----Original Message----- X-from: eq23-at-rice.edu [mailto:eq23-at-rice.edu] Sent: August 22, 2007 4:38 PM To: maryflet-at-interchange.ubc.ca
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying
please copy both eq23-at-rice.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: eq23-at-rice.edu Name: Elizabeth
Organization: Rice university
Title-Subject: [Filtered] TEM--any concerns while pregnant?
Question: Greetings,
A graduate student, I'm a frequent user on the Jeol 2010 and 1230 TEMs at the university. I also found out that I'm 2 months pregnant. Should I be concern of any x-ray or other ionizing energy that may leak from the TEMs? Should I wait after the first trimester (a few more weeks) to get back on the TEM? Does anybody have any good information or advice?
-- David, Haven't used any for e-beam resist, but www.gelest.com would be a source for lab size amounts. They'd also be a good place to ask for advice. HTH
Rob Bowen
Robert C. Bowen Research Scientist Caddock Electronics, Inc rob.bowen-at-caddock.com http://www.caddock.com
} From: {freym2-at-rpi.edu} } Reply-To: {freym2-at-rpi.edu} } Date: Thu, 23 Aug 2007 09:28:32 -0500 } To: {rob.bowen-at-caddock.com} } Subject: [Microscopy] e-Beam lithography Negative Resists } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Has anyone on the list had experience using H.S.Q. (hydrogen silsesquioxane) } as a negative e-beam resist? If so has it been useful, any tips you can } share as to ease of use, a good source for the material. Thanks for any help } you can offer. } } David } } } M. David Frey } Senior Application Engineer } Rensselaer Polytechnic Institute } Low Center for Industrial Inn. } Center for Integrated Electronics } 110 8th Street } CII 4161 (Office) } CII 6015 (Packages and Mail) } Troy, NY 12180 } 518-276-3323 (office) } 518-698-2288 (mobile) } } } } } ==============================Original Headers============================== } 7, 23 -- From freym2-at-rpi.edu Thu Aug 23 09:22:54 2007 } 7, 23 -- Received: from smtp6.server.rpi.edu (smtp6.server.rpi.edu } [128.113.2.226]) } 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l7NEMsrU028575 } 7, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 09:22:54 -0500 } 7, 23 -- Received: from Frey (sponge-bob-49.dynamic.rpi.edu [128.113.222.88]) } 7, 23 -- by smtp6.server.rpi.edu (8.13.1/8.13.1) with ESMTP id l7NEMooW032158 } 7, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 10:22:54 -0400 } 7, 23 -- From: "M. David Frey" {freym2-at-rpi.edu} } 7, 23 -- To: {Microscopy-at-microscopy.com} } 7, 23 -- Subject: e-Beam lithography Negative Resists } 7, 23 -- Date: Thu, 23 Aug 2007 10:22:45 -0400 } 7, 23 -- Message-ID: {002601c7e591$132b1e40$58de7180-at-Frey} } 7, 23 -- MIME-Version: 1.0 } 7, 23 -- Content-Type: text/plain; } 7, 23 -- charset="US-ASCII" } 7, 23 -- Content-Transfer-Encoding: 7bit } 7, 23 -- X-Mailer: Microsoft Office Outlook 11 } 7, 23 -- Thread-Index: AcflkRLVJ97W8rjmQA2TlxYRx1aARg== } 7, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 } 7, 23 -- X-RPI-SA-Score: undef - spam scanning disabled } 7, 23 -- X-CanItPRO-Stream: default } 7, 23 -- X-Canit-Stats-ID: Bayes signature not available } 7, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 128.113.2.226 } ==============================End of - Headers==============================
On Aug 22, 2007, at 4:30 PM, eq23-at-rice.edu wrote:
} A graduate student, I'm a frequent user on the Jeol 2010 and 1230 } TEMs at the university. I also found out that I'm 2 months } pregnant. Should I be concern of any x-ray or other ionizing } energy that may leak from the TEMs? Should I wait after the first } trimester (a few more weeks) to get back on the TEM? Does anybody } have any good information or advice?
Dear Elizabeth, Modern EMs are well-designed so that they emit very little radiation, and the safety officer at your institution should make frequent checks to see that the EM meets the spec. That said, you could wear a dosimeter to measure your exposure. I would hope that you would be reassured that your exposure is minimal--probably below the limit of detection. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 5, 22 -- From tivol-at-caltech.edu Thu Aug 23 13:34:50 2007 5, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7NIYnkf018753 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Thu, 23 Aug 2007 13:34:50 -0500 5, 22 -- Received: from water-dog.its.caltech.edu (water-dog [192.168.1.26]) 5, 22 -- by wood-ox-postvirus (Postfix) with ESMTP id 306E113BB8 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Thu, 23 Aug 2007 11:34:49 -0700 (PDT) 5, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 5, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id DBFD013BC6 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Thu, 23 Aug 2007 11:32:15 -0700 (PDT) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v752.3) 5, 22 -- In-Reply-To: {200708222330.l7MNUrge021960-at-ns.microscopy.com} 5, 22 -- References: {200708222330.l7MNUrge021960-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {0C7A0FEC-5487-445E-8BA9-8DC7CD3E16AD-at-caltech.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 22 -- Subject: Re: [Microscopy] viaWWW: TEM--any concerns while pregnant 5, 22 -- Date: Thu, 23 Aug 2007 19:32:43 -0700 5, 22 -- To: microscopy-at-msa.microscopy.com 5, 22 -- X-Mailer: Apple Mail (2.752.3) 5, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.4.5 ==============================End of - Headers==============================
Many, many moons ago, I had our TEM surveyed by our radiation safety guys when I was pregnant (JEOL 2000FX) and what they discovered was that there was absolutely no radiation leakage EXCEPT when the condenser aperture was out. Ours has two sets of apertures - top hat and regular and there is an intermediate setting with no aperature in. We tried messing up the centering on the apertures and everything to see if anything leaked and it didn't except as I stated above. I recommend a similar check for you. I'm sure someone on campus at Rice has a detector. I worked at Hanford laboratory and had some extra radiation training and developing cells are very sensitive to radiation. Along the same lines, you need to be very careful about what chemicals and biological hazards you handle when you do sample preparation. We've had lots of female graduate students, employees and faculty pregnant in my 18 years here and I've never had trouble getting people to cover for a pregnant woman. Of course, I'm in the south and southern gentlemen are wonderfully polite so maybe that is why it was so easy for us!! I'm sure there are rules that protect you from being forced to do stuff that is hazardous now if you find out there are issues in your lab. Check with your safety department and congratulations.....
-----Original Message----- X-from: eq23-at-rice.edu [mailto:eq23-at-rice.edu] Sent: Wednesday, August 22, 2007 6:38 PM To: Robin D Griffin
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both eq23-at-rice.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: eq23-at-rice.edu Name: Elizabeth
Organization: Rice university
Title-Subject: [Filtered] TEM--any concerns while pregnant?
Question: Greetings,
A graduate student, I'm a frequent user on the Jeol 2010 and 1230 TEMs at the university. I also found out that I'm 2 months pregnant. Should I be concern of any x-ray or other ionizing energy that may leak from the TEMs? Should I wait after the first trimester (a few more weeks) to get back on the TEM? Does anybody have any good information or advice?
I am trying to locate the C.M. Taylor Corporation, specialists in microbeam standards. We were given one of their standards (NO. 202) but need a map of the minerals present. Any help would be appreciated.
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kdunnerj-at-mdanderson.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kdunnerj-at-mdanderson.org Name: Kenneth Dunner Jr
Organization: MD Anderson Cancer Center
Title-Subject: [Filtered] Osmium tetroxide on Lowicryl Sections
Question: A colleague I know wants to know has anyone used osmium tetroxide on post embedded immunogold labeled Lowicryl sections to make microtubules or other membranes stand out?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dlowry-at-asu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dlowry-at-asu.edu Name: David Lowry
Organization: Arizona State University
Title-Subject: [Filtered] negative staining problem
Question: I believe there was a previous discussion on the List concerning this issue, but I could not locate the subject in the archives. I am having a negative staining problem with what is commonly referred to as 'champagning' or tiny bubble-like areas around specimens and other particles on the grid. I observe this frequently with all stains I use--UA, PTA and amm molybdate--and at different concentrations and pH values. It also doesn't appear to be affected by surfactants or time duration of stain application. I seem to recall this phenomenon is not well understood as to its cause, but I was wondering if anyone may have any information or ideas on how to minimize or eliminate this from occurring. Thanks,
if the electron microscopes are well maintained and have the usual safety checks, there should be no problem during normal operation.
I would be much more concerned about chemical and biohazard risks in the lab, which of course should normally be assessed.
In the UK it would be possible to ask if a risk assessment for the lab would identify specific risks to pregnant women.
Congratulations and I hope all goes well in March/April next year.
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: eq23-at-rice.edu
Stephane asks ...
} - why is the integration of the peaks in the EDX spectrum } called "weight %"? } - Does the intensity if the peaks in EDX depend on the atomic } weight? In other words, do heavier elements produce more } x-rays than lighter elements? It is a hard for me to believe } this, because electron shells are the same for light and } heavy elements (a K shell is a K shell), however it is the } only reason I can see to calculate an atom% value.
The EPMA technique, whether EDX or WDX, is more sensitive to mass% than atom%. I know this is somewhat counter-intuitive and I remember having the same conceptual problems myself. However, you can do simple tests with stoichometric compounds that include heavy and lighter atomic numbers. A good example would be to compare Fe metal and FeO. If you integrate the Fe Ka peak for both you'll see that for FeO it almost directly calculates the mass fraction rather than the atom fraction.
Therefore mass fractions are always the direct result of EPMA, while atom fractions are calculated secondarily. Most analysts will always report the mass% because it will include the actual total of all elements, while the atom% will always be a result of having normalized to 100%.
HTH & cheerios, michael shaffer :o) SEM-MLA Research Coodinator INCO Innovation Centre Memorial University St. John's Newfoundland http://www.mun.ca/creait/maf/
==============================Original Headers============================== 6, 21 -- From michael-at-shaffer.net Fri Aug 24 06:47:12 2007 6, 21 -- Received: from n007.sc1.he.tucows.com (smtpout1468.sc1.he.tucows.com [64.97.157.168]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7OBlCKF016365 6, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 06:47:12 -0500 6, 21 -- Received: from roamingwolf (205.251.83.78) by n007.sc1.he.tucows.com (7.2.069.1) (authenticated as michael-at-shaffer.net) 6, 21 -- id 465670BB0101E364; Fri, 24 Aug 2007 11:47:11 +0000 6, 21 -- From: "michael shaffer" {michael-at-shaffer.net} 6, 21 -- To: {nizets2-at-yahoo.com} 6, 21 -- Cc: "MSA Microscopy list" {Microscopy-at-microscopy.com} 6, 21 -- References: {200708230723.l7N7N0cG010947-at-ns.microscopy.com} 6, 21 -- Subject: RE: [Microscopy] SEM: wt% or atom% ? 6, 21 -- Date: Fri, 24 Aug 2007 09:17:47 -0230 6, 21 -- Message-ID: {001201c7e644$989848b0$6401a8c0-at-CREAIT.MUN.CA} 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: text/plain; 6, 21 -- charset="us-ascii" 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- X-Mailer: Microsoft Office Outlook 11 6, 21 -- In-Reply-To: {200708230723.l7N7N0cG010947-at-ns.microscopy.com} 6, 21 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 6, 21 -- Thread-index: AcflVbLp3kDIq/XVR0u1mv7Vhlw9jQA7Xfcw ==============================End of - Headers==============================
We currently have and use heavily an older scientific microwave for EM sample preparation. It currently sits in a fume hood so that not only will vapors be vented efficiently from the microwave vent but also when the door is opened. Samples are handled exclusively in the hood and all reagents are kept in the hood adjacent to the microwave so that at no time does hazardous materials have to be transported through the open room during sample preparation.
I have been asked to relinquish the hood. This will require placing the microwave on the bench in an open lab. The top of the microwave would have to be vented into an adjacent hood and all reagents and samples would need to be moved between this hood and the microwave through the open lab space.
My concern is a safety one. We already had one technician who had an adverse reaction to fumes (gloved fingers swelling, numbness) when we tried a similar configuration some years ago. We had the microwave on a table at 90o from the hood so the distance to move samples and chemicals between the two were as short as possible. The microwave vent tube was run into the hood. This technician has not had any adverse reactions since the microwave and all processing is done in the same hood.
I would like to hear from others as to what your feeling are concerning the safety issues involved and what you feel is the appropriate way to deal with these safety issues.
Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 8, 21 -- From dsherman-at-purdue.edu Fri Aug 24 09:10:59 2007 8, 21 -- Received: from 1061exfe02.adpc.purdue.edu (1061exfe02.adpc.purdue.edu [128.210.63.223]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7OEAxtt031884 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 09:10:59 -0500 8, 21 -- Received: from exchange.purdue.edu ([172.21.6.24]) by 1061exfe02.adpc.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 21 -- Fri, 24 Aug 2007 10:10:59 -0400 8, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 8, 21 -- Fri, 24 Aug 2007 14:10:59 +0000 8, 21 -- User-Agent: Microsoft-Entourage/11.3.6.070618 8, 21 -- Date: Fri, 24 Aug 2007 10:10:58 -0400 8, 21 -- Subject: Safety issues with microwave 8, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 8, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 8, 21 -- Message-ID: {C2F45F32.20DDC%dsherman-at-purdue.edu} 8, 21 -- Thread-Topic: Safety issues with microwave 8, 21 -- Thread-Index: AcfmWJez1fa55FJLEdyWGQARJN08Mg== 8, 21 -- Mime-version: 1.0 8, 21 -- Content-type: text/plain; 8, 21 -- charset="US-ASCII" 8, 21 -- Content-transfer-encoding: 7bit 8, 21 -- X-OriginalArrivalTime: 24 Aug 2007 14:10:59.0157 (UTC) FILETIME=[98645050:01C7E658] ==============================End of - Headers==============================
While filing your summary I came across the following technical article that describes the gold on carbon sample as well as others.
Humenansky, John. (1987). Manufacture and use of test samples to adjust and evaluate the SEM, TEM and STEM. EMSA Bulletin 17:1 68-72.
Hope all is well, Louie
kenconverse-at-qualityimages.biz wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear listers, } Here are the replies I got. Very helpful. Thank you all. } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } I inadvertently made a Al/W dendrite sample by heating Al foil (or } wire, I don't remember) in a W coil basket in my vacuum evaporator } when making a telescope mirror. The Al embrittles the W and as you } evaporate the Al, eventually the W wire breaks and you are left with } a glob of Al in the basket. Looks great in BSE mode. } Henk } **************************************************************************** } *** } } I have made the Al-W dendritic structures in an evaporator. From my } observations; } 1. Use excess Al for the charge. } 2. Heat the charge until the Al is molten. I usually cut-back on heating } power, you need just enough to keep the Al molten for about 3 minutes, } without evaporating it all away. 3. Cool the molten blob rapidly by just } shutting off the heater power. 4. The dendritic structures are located in } small "pockets" near the top of the now solidified blob. } Joseph M. Oparowski } **************************************************************************** } ***** } } I don't know the answer to your question but as far as dendritic grow is } concerned, here is what I do know... } } Dendritic growth is a process where one constituent of an alloy begins to } solidfy before another. This leaves the liquid with a compostion lower in } the first constituent and higher in the second. The process of the growing } is limited by a diffusion process of the two components in the liquid. } This means you get smaller dendrites with faster cooling rates and larger } dendrites with slower cooling rates. However, if you cool fast enough, you } can't get the diffusion process happening, and so then you don't get any } dendritic growth. If you cool really slow, well, then it depends upon the } alloy.... } } You have asked about Al/W. Now those two have really different melting } points so I'd think you'd get darned good dentritic growth with those two } just about no matter how you cooled it... If you went slow, I'd bet you'd } get a lot of interdendritic shrinkage cavities though.... } } Also, I don't know how big of a structure you want, but it would be my } guess that you could cool that alloy pretty quick and have some nice } dendritic growth... } } What did you do the first time you tried that? And how did it turn out? } } If someone does answer you with a lot more info, I'd like to find out } myself...If you don't mind sending along the info.. } } Dj } *************************************************************************** } } It has been a while since I made these, but I will try to remember. To make } the best gold-on-carbon islands, you evaporate pure gold onto polished } spectrographic-grade graphite, then post-heat the sample to make the islands } coalesce a bit. Takes a few tries to get both the amount of gold and the } post heating right. Some of the ones I've seen also have a bit of evaporated } tin on top of that; it makes little balls decorating the big balls and is } better for a FESEM and high resolution testing. To make the Al-W phase, just } put pure Al in a W basket and heat it up until the aluminum melts, then } evaporates. Soon after that, the W basket will break at one of the arms, } because the Al-W alloy formed is very brittle. The basket with the cooled Al } in it will show the dendrites and a pretty Al-W phase. I'm not sure if } cooling fast or slow matters, it cools pretty fast when the wire breaks. } Good luck, } } Mary Fletcher } **************************************************************************** } ** } } I once saw a beautiful, albeit unintentional, resolution sample of someone } who evaporated gold onto an anthracite coal sample. I thought that the gold } on carbon were made by evaporating gold onto a warm polished graphite } surface. The gold doesn't wet the carbon and forms islands. If you stop } the deposition just before coalescence of the Au islands, you have your } resolution sample. Gold has a tendency to films by island coalescence on } most substrates. That is why it has a rough structure and is not a good } choice for high resolution SEM imaging. } } For an alloy that forms a dendritic structure, the microstructure will be } finer with a higher cooling rate. } } Scott D. Walck, Ph.D. } **************************************************************************** } **** } } } } } _________________________________________________________________ } Need personalized email and website? Look no further. It's easy } with Doteasy $0 Web Hosting! Learn more at www.doteasy.com } } } ==============================Original Headers============================== } 21, 30 -- From kenconverse-at-qualityimages.biz Thu Aug 23 05:57:45 2007 } 21, 30 -- Received: from dpmailmta01.doteasy.com (dpmailmta01-02.doteasy.com [65.61.218.2]) } 21, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7NAviF3027802 } 21, 30 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 23 Aug 2007 05:57:44 -0500 } 21, 30 -- Received: from qualityimages.biz (unverified [192.168.101.16]) } 21, 30 -- by dpmailmta01.doteasy.com (DEO) with ESMTP id 144594340-1814644 } 21, 30 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 23 Aug 2007 04:22:06 -0700 } 21, 30 -- Received: from Ken [72.227.100.25] by qualityimages.biz with ESMTP } 21, 30 -- (SMTPD32-8.05) id A7C66C5800F8; Thu, 23 Aug 2007 03:56:06 -0700 } 21, 30 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } 21, 30 -- To: "'MSA Listserver'" {Microscopy-at-MSA.Microscopy.Com} } 21, 30 -- Subject: Re: [Microscopy] recipe for Au on C and Al/W dendrites } 21, 30 -- Date: Thu, 23 Aug 2007 06:57:25 -0400 } 21, 30 -- Message-ID: {000e01c7e574$6513ec40$6401a8c0-at-Ken} } 21, 30 -- MIME-Version: 1.0 } 21, 30 -- Content-Type: text/plain; } 21, 30 -- charset="us-ascii" } 21, 30 -- X-Priority: 3 (Normal) } 21, 30 -- X-MSMail-Priority: Normal } 21, 30 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 } 21, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 } 21, 30 -- Thread-Index: AcfldGN3YmW81roMQxCHjXx8Wj9zSQ== } 21, 30 -- Importance: Normal } 21, 30 -- X-IMSTrailer: __IMail_7__ } 21, 30 -- X-SpamDetect: ****: 4.100000 SpamUrl=4.1 } 21, 30 -- X-SpamUrl: doteasy.com } 21, 30 -- X-IP-stats: Incoming Last 0, First 19, in=255648, out=0, spam=0 ip=192.168.101.16 } 21, 30 -- X-Originating-IP: 192.168.101.16 } 21, 30 -- Content-Transfer-Encoding: 8bit } 21, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7NAviF3027802 } ==============================End of - Headers============================== }
-- Louie Kerr Research and Education Support Coordinator Marine Biological Laboratory 7 MBL Street Woods Hole, MA 02543 508-289-7273 508-540-6902 (FAX) 508-292-0289 (Cell phone)
VISIT OUR WEB SITES: http://www.mbl.edu/ http://www.courses.mbl.edu/
==============================Original Headers============================== 8, 21 -- From lkerr-at-mbl.edu Fri Aug 24 09:14:10 2007 8, 21 -- Received: from adios.mbl.edu (adios.MBL.EDU [128.128.172.10]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7OEEATs003477 8, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 09:14:10 -0500 8, 21 -- Received: from [128.128.161.46] (dhcp16146.MBL.EDU [128.128.161.46]) 8, 21 -- by adios.mbl.edu (Postfix) with ESMTP id 59D2A1CE413; 8, 21 -- Fri, 24 Aug 2007 10:14:11 -0400 (EDT) 8, 21 -- Message-ID: {46CEE7AE.10909-at-mbl.edu} 8, 21 -- Date: Fri, 24 Aug 2007 10:14:06 -0400 8, 21 -- From: Louis Kerr {lkerr-at-mbl.edu} 8, 21 -- Reply-To: lkerr-at-mbl.edu 8, 21 -- Organization: Marine Biological Laboratory 8, 21 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 8, 21 -- X-Accept-Language: en-us, en 8, 21 -- MIME-Version: 1.0 8, 21 -- To: kenconverse-at-qualityimages.biz, Microscopy-at-microscopy.com 8, 21 -- Subject: Re: [Microscopy] Re: recipe for Au on C and Al/W dendrites 8, 21 -- References: {200708231059.l7NAxOC8029476-at-ns.microscopy.com} 8, 21 -- In-Reply-To: {200708231059.l7NAxOC8029476-at-ns.microscopy.com} 8, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed 8, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi Deb. That microwave should be under a portable hooded enclosure which can be vented to the main hood, with sufficient adjoining work space for reagent handling. Check out the vented bench top workstations from Flow Sciences: http://www.flowsciences.com
Also, you didn't state the protective wear your colleague was wearing. I highly recommend the use of non-powdered Nitrile gloves (double up and discard the outer layer if they become fairly contaminated with resin).
Best regards,
Walter F. Bobrowski Sr. Scientist Pfizer Global R&D 2800 Plymouth Rd. Ann Arbor, MI 48105
TEL: 734-622-7814 FAX: 734-622-5718
"Like the strength of a steel rod, the true character of a person can only be known under extreme stress." - Leslie Fieger
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Friday, August 24, 2007 10:20 AM To: Bobrowski, Walter
Folks,
We currently have and use heavily an older scientific microwave for EM sample preparation. It currently sits in a fume hood so that not only will vapors be vented efficiently from the microwave vent but also when the door is opened. Samples are handled exclusively in the hood and all reagents are kept in the hood adjacent to the microwave so that at no time does hazardous materials have to be transported through the open room during sample preparation.
I have been asked to relinquish the hood. This will require placing the microwave on the bench in an open lab. The top of the microwave would have to be vented into an adjacent hood and all reagents and samples would need to be moved between this hood and the microwave through the open lab space.
My concern is a safety one. We already had one technician who had an adverse reaction to fumes (gloved fingers swelling, numbness) when we tried a similar configuration some years ago. We had the microwave on a table at 90o from the hood so the distance to move samples and chemicals between the two were as short as possible. The microwave vent tube was run into the hood. This technician has not had any adverse reactions since the microwave and all processing is done in the same hood.
I would like to hear from others as to what your feeling are concerning the safety issues involved and what you feel is the appropriate way to deal with these safety issues.
Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 8, 21 -- From dsherman-at-purdue.edu Fri Aug 24 09:10:59 2007 8, 21 -- Received: from 1061exfe02.adpc.purdue.edu (1061exfe02.adpc.purdue.edu [128.210.63.223]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7OEAxtt031884 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 09:10:59 -0500 8, 21 -- Received: from exchange.purdue.edu ([172.21.6.24]) by 1061exfe02.adpc.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 21 -- Fri, 24 Aug 2007 10:10:59 -0400 8, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 8, 21 -- Fri, 24 Aug 2007 14:10:59 +0000 8, 21 -- User-Agent: Microsoft-Entourage/11.3.6.070618 8, 21 -- Date: Fri, 24 Aug 2007 10:10:58 -0400 8, 21 -- Subject: Safety issues with microwave 8, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 8, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 8, 21 -- Message-ID: {C2F45F32.20DDC%dsherman-at-purdue.edu} 8, 21 -- Thread-Topic: Safety issues with microwave 8, 21 -- Thread-Index: AcfmWJez1fa55FJLEdyWGQARJN08Mg== 8, 21 -- Mime-version: 1.0 8, 21 -- Content-type: text/plain; 8, 21 -- charset="US-ASCII" 8, 21 -- Content-transfer-encoding: 7bit 8, 21 -- X-OriginalArrivalTime: 24 Aug 2007 14:10:59.0157 (UTC) FILETIME=[98645050:01C7E658] ==============================End of - Headers==============================
---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately.
==============================Original Headers============================== 23, 31 -- From Walter.Bobrowski-at-pfizer.com Fri Aug 24 09:31:25 2007 23, 31 -- Received: from gromsgom01.pfizer.com (gromsgo.pfizer.com [148.168.224.84]) 23, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7OEVP3m023095 23, 31 -- for {microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 09:31:25 -0500 23, 31 -- Received: from mopamrexc01.amer.pfizer.com (mopamrexc01.pfizer.com [170.116.32.254]) 23, 31 -- by gromsgom01.pfizer.com (8.13.7/8.13.7) with ESMTP id l7OEV9vH008040; 23, 31 -- Fri, 24 Aug 2007 10:31:25 -0400 23, 31 -- Received: from mopamrexc03.amer.pfizer.com ([170.116.30.69]) by mopamrexc01.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.1830); 23, 31 -- Fri, 24 Aug 2007 10:31:22 -0400 23, 31 -- Received: from anaamrexm01.amer.pfizer.com ([162.48.101.10]) by mopamrexc03.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.1830); 23, 31 -- Fri, 24 Aug 2007 10:31:22 -0400 23, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 23, 31 -- Content-class: urn:content-classes:message 23, 31 -- MIME-Version: 1.0 23, 31 -- Content-Type: text/plain; 23, 31 -- charset="us-ascii" 23, 31 -- Subject: RE: [Microscopy] Safety issues with microwave 23, 31 -- Date: Fri, 24 Aug 2007 10:31:18 -0400 23, 31 -- Message-ID: {CF89AF6ABD93B746A55BCCD9480A4B3D09987504-at-anaamrexm01.amer.pfizer.com} 23, 31 -- In-Reply-To: {200708241419.l7OEJbgi016214-at-ns.microscopy.com} 23, 31 -- X-MS-Has-Attach: 23, 31 -- X-MS-TNEF-Correlator: 23, 31 -- Thread-Topic: [Microscopy] Safety issues with microwave 23, 31 -- Thread-Index: AcfmWc4hnBBi1VuGQryUX+h4+JJKdAAAEn4A 23, 31 -- From: "Bobrowski, Walter" {Walter.Bobrowski-at-pfizer.com} 23, 31 -- To: {dsherman-at-purdue.edu} , {microscopy-at-microscopy.com} 23, 31 -- X-OriginalArrivalTime: 24 Aug 2007 14:31:22.0130 (UTC) FILETIME=[71572F20:01C7E65B] 23, 31 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=4.65.5502:2.3.11,1.2.37,4.0.164 definitions=2007-08-24_03:2007-08-23,2007-08-24,2007-08-24 signatures=0 23, 31 -- X-Proofpoint-Spam-Reason: safe 23, 31 -- Content-Transfer-Encoding: 8bit 23, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7OEVP3m023095 ==============================End of - Headers==============================
We have similar situation to your original situation. Our main processing microwave sits right next to the hood and is vented through a properly taped duct system. We try to train our clients to always use the vacuum chamber, regardless if they are applying vacuum or not. The chamber can be closed during the transfer from the hood to the microwave.
Our main issue is getting people to clean up after themselves. If anyone has a nice way to convince your clients to clean up after they finish processing I would love to hear it. The usual threats of taking away privileges does not seem to work.
Garnet
-- Garnet Martens
Research Manager BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
phone 604-822-3354
==============================Original Headers============================== 7, 29 -- From gmartens-at-interchange.ubc.ca Fri Aug 24 10:14:31 2007 7, 29 -- Received: from mr1.mail-relay.ubc.ca (mr1.mail-relay.ubc.ca [137.82.45.1]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7OFEV1U003262 7, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 24 Aug 2007 10:14:31 -0500 7, 29 -- X-Ubc-Received: from mr1.mail-relay.ubc.ca (localhost [127.0.0.1]) 7, 29 -- by localhost (Postfix) with SMTP id BB276ECA9 7, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 24 Aug 2007 08:14:30 -0700 (PDT) 7, 29 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 7, 29 -- by mr1.mail-relay.ubc.ca (Postfix) with ESMTP 7, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 24 Aug 2007 08:14:30 -0700 (PDT) 7, 29 -- Received: from [137.82.85.216] (0511.emlab.ubc.ca [137.82.85.216]) 7, 29 -- by smtp.interchange.ubc.ca 7, 29 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 7, 29 -- with ESMTPA id {0JNA003UVAC5GU-at-smtp.interchange.ubc.ca} for 7, 29 -- Microscopy-at-MSA.Microscopy.Com; Fri, 24 Aug 2007 08:14:29 -0700 (PDT) 7, 29 -- Date: Fri, 24 Aug 2007 08:15:14 -0700 7, 29 -- From: Garnet Martens {gmartens-at-interchange.ubc.ca} 7, 29 -- Subject: Re: [Microscopy] Safety issues with microwave 7, 29 -- In-reply-to: {200708241415.l7OEFouf008498-at-ns.microscopy.com} 7, 29 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 7, 29 -- Message-id: {a06240805c2f4a52991fc-at-[137.82.85.216]} 7, 29 -- MIME-version: 1.0 7, 29 -- Content-type: text/plain; format=flowed; charset=us-ascii 7, 29 -- References: {200708241415.l7OEFouf008498-at-ns.microscopy.com} 7, 29 -- X-UBC-Scanned: Sophos PureMessage 5.3.3.310218, Antispam-Engine: 2.5.2.311128, Antispam-Data: 2007.8.24.74423 7, 29 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 7, 29 -- X-PerlMx-Spam: Probability=7%, Report=BODY_SIZE_800_899 0, __C230066_P5 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0 7, 29 -- X-Spam-Level: 7, 29 -- X-Spam-Flag: No ==============================End of - Headers==============================
Kenneth- I have never tried this, but I would be surpised if it would work. Lowicrl resin is itself quite reactive, and I would expect it to have reacted with a lot of the primary and secondary amines that OsO4 reacts with. This may be worth a try though. It has never been reported, to my knowledge, so it would be new knowledge. Carol
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- Carol A. Heckman, Ph.D. Director, Center for Microscopy & Microanalysis and Professor of Biological Sciences Bowling Green State University Bowling Green, OH 43403 USA website: http://www.bgsu.edu/departments/biology/facilities/MnM
==============================Original Headers============================== 4, 18 -- From heckman-at-bgnet.bgsu.edu Fri Aug 24 10:17:12 2007 4, 18 -- Received: from smtp02.bgsu.edu (smtp02.bgsu.edu [129.1.5.18]) 4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7OFH42b007433 4, 18 -- for {microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 10:17:06 -0500 4, 18 -- Received: from [129.1.85.81] (dhcp-85-81.bgsu.edu [129.1.85.81]) 4, 18 -- by smtp02.bgsu.edu (Switch-3.2.2/Switch-3.1.6) with ESMTP id l7OFGx8E025891; 4, 18 -- Fri, 24 Aug 2007 11:17:00 -0400 (EDT) 4, 18 -- Mime-Version: 1.0 4, 18 -- X-Sender: heckman-at-mailstore.bgsu.edu 4, 18 -- Message-Id: {p04320403c2f4d07727b3-at-[129.1.85.81]} 4, 18 -- In-Reply-To: {200708232246.l7NMkDk0031806-at-ns.microscopy.com} 4, 18 -- References: {200708232246.l7NMkDk0031806-at-ns.microscopy.com} 4, 18 -- Date: Fri, 24 Aug 2007 11:16:59 -0700 4, 18 -- To: kdunnerj-at-mdanderson.org, 4, 18 -- "Microscopy Listserver" {microscopy-at-microscopy.com} 4, 18 -- From: Carol Heckman {heckman-at-bgnet.bgsu.edu} 4, 18 -- Subject: Re: [Microscopy] viaWWW: Osmium tetroxide on Lowicryl Sections 4, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
How about billing them for the cleanup time (at the extra-special unsubsidized billing rate)? Call it a 'service' for those too busy to be good citizens. David
On Aug 24, 2007, at 8:17 AM, gmartens-at-interchange.ubc.ca wrote:
} Our main issue is getting people to clean up after themselves. If } anyone has a nice way to convince your clients to clean up after they } finish processing I would love to hear it. The usual threats of } taking away privileges does not seem to work.
==============================Original Headers============================== 5, 22 -- From Elliott-at-arizona.edu Fri Aug 24 10:29:22 2007 5, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7OFTLXd026843 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 24 Aug 2007 10:29:22 -0500 5, 22 -- Received: from gandalfs_amavis (amavis5.email.arizona.edu [10.0.0.208]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 66B86D44EC 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 24 Aug 2007 08:29:21 -0700 (MST) 5, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 2F375D3B9A 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 24 Aug 2007 08:29:20 -0700 (MST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 5, 22 -- In-Reply-To: {200708241517.l7OFHteV009198-at-ns.microscopy.com} 5, 22 -- References: {200708241517.l7OFHteV009198-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {1EF3F525-DB8C-420C-888F-0B13266A9EE4-at-arizona.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: David Elliott {Elliott-at-arizona.edu} 5, 22 -- Subject: cleaning up [was - Safety issues with microwave] 5, 22 -- Date: Fri, 24 Aug 2007 08:29:19 -0700 5, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 22 -- X-Mailer: Apple Mail (2.752.2) 5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
2 minutes in 2% UA and 30 seconds in lead citrate give strong staining with LR and Lowicryl. This will give negative contrast on membranes and positive contrast of microtubules. Ribosomes stand out very clearly, too.
Also, I would recommend 2% (unbuffered) potassium permanganate for 2 minutes. This will give good positive contrast of membranes. (My experiences are mostly with plant material, however.)
Pre-embedding treatment of tissue with 2% UA overnight before lowicryl will preserve/stabilize the membrane as well as give them some contrast after sectioning.
Kevin Vaughn
==============================Original Headers============================== 6, 30 -- From Andrew.Bowling-at-ARS.USDA.GOV Fri Aug 24 10:40:47 2007 6, 30 -- Received: from messagescreen4.ars.usda.gov (messagescreen4.ars.usda.gov [199.133.180.151]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7OFelxp006272 6, 30 -- for {microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 10:40:47 -0500 6, 30 -- Received: from CO-MAILBH-01.ARSNET.ARS.USDA.GOV ([199.133.183.226]) 6, 30 -- by messagescreen4.ars.usda.gov (8.13.8/8.13.8) with ESMTP id l7OFXjhp012921 6, 30 -- for {microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 10:40:47 -0500 6, 30 -- Received: from CO-MAIL-03.ARSNET.ARS.USDA.GOV ([10.100.2.202]) by CO-MAILBH-01.ARSNET.ARS.USDA.GOV with Microsoft SMTPSVC(6.0.3790.3959); 6, 30 -- Fri, 24 Aug 2007 09:39:20 -0600 6, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 30 -- Content-class: urn:content-classes:message 6, 30 -- MIME-Version: 1.0 6, 30 -- Content-Type: text/plain; 6, 30 -- charset="US-ASCII" 6, 30 -- Subject: [Microscopy] Re: viaWWW: Osmium tetroxide on Lowicryl Sections 6, 30 -- Date: Fri, 24 Aug 2007 09:39:20 -0600 6, 30 -- Message-ID: {8017F94146BF634DA9414E4B9088525B28E768-at-CO-MAIL-03.ARSNET.ARS.USDA.GOV} 6, 30 -- X-MS-Has-Attach: 6, 30 -- X-MS-TNEF-Correlator: 6, 30 -- Thread-Topic: [Microscopy] Re: viaWWW: Osmium tetroxide on Lowicryl Sections 6, 30 -- Thread-Index: AcfmbJwUQUB6Ny83R+6deBaztiY6bw== 6, 30 -- From: "Bowling, Andrew" {Andrew.Bowling-at-ARS.USDA.GOV} 6, 30 -- To: {microscopy-at-microscopy.com} 6, 30 -- X-OriginalArrivalTime: 24 Aug 2007 15:39:20.0717 (UTC) FILETIME=[F05E2BD0:01C7E664] 6, 30 -- X-MessageScreenMessageID: 1187970047.465704.1254.1442480164 6, 30 -- X-MessageScreenContentScore: Score of 0 assigned to Content 6, 30 -- X-MessageScreenUCEScore: Score of 0 assigned to UCE 6, 30 -- X-MessageScreen: Analyzed by IntelliReach MessageScreen(tm) 6, 30 -- Content-Transfer-Encoding: 8bit 6, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7OFelxp006272 ==============================End of - Headers==============================
Has anyone had contamination problems when looking at PEGDA (polyethylene glycol-diacrylate) hydrogels using SEM?
Many thanks!
Tom
Tom Stephens Assistant Research Scientist Microscopy and Imaging Center Texas A&M University College Station, TX 77843 phone:979-845-1129 fax:979-847-8933 email:tstephen-at-mic.tamu.edu
==============================Original Headers============================== 6, 20 -- From tstephen-at-mic.tamu.edu Fri Aug 24 11:44:35 2007 6, 20 -- Received: from sr-7-int.cis.tamu.edu (smtp-relay.tamu.edu [165.91.22.120]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7OGiZ0a019579 6, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 24 Aug 2007 11:44:35 -0500 6, 20 -- Received: from localhost (localhost.tamu.edu [127.0.0.1]) 6, 20 -- by sr-7-int.cis.tamu.edu (Postfix) with ESMTP id 40B5A51891 6, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 24 Aug 2007 11:44:35 -0500 (CDT) 6, 20 -- Received: from jntcspc.mic.tamu.edu (cyn6400.tamu.edu [165.91.109.143]) 6, 20 -- by sr-7-int.cis.tamu.edu (Postfix) with ESMTP id 96698529F7 6, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 24 Aug 2007 11:44:34 -0500 (CDT) 6, 20 -- Message-Id: {5.2.0.9.0.20070824112621.02df2ad8-at-mic.tamu.edu} 6, 20 -- X-Sender: tstephen-at-mic.tamu.edu (Unverified) 6, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.0.9 6, 20 -- Date: Fri, 24 Aug 2007 11:45:40 -0500 6, 20 -- To: Microscopy-at-Microscopy.Com 6, 20 -- From: Tom Stephens {tstephen-at-mic.tamu.edu} 6, 20 -- Subject: SEM of hydrogels, anyone have contamination problems? 6, 20 -- Mime-Version: 1.0 6, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 20 -- X-Virus-Scanned: amavisd-new at tamu.edu ==============================End of - Headers==============================
I have observed gold islands before that resulted from heating gold-coated silica at several hundred degrees (a client's project). Prompted by the current discussion and our own need for such a sample, I decided to go low-tech and try replicating the result with gold on carbon.
I sputtered several garden-variety graphite stubs with about 20 nm of gold and heated the samples in a muffle furnace at about 500 C for about two hours. I was busy with other things and did not control the time well. I also did not experiment with the temperature or the thickness of gold. (I probably should go read Humenansky's note.)
I got decent islands of gold on all samples. I have posted images on our server at ftp://www.marl.iastate.edu/Gold-on-C/. The images were comparable to those from our service engineers gold-on-carbon samples. Now I wonder why I didn't try this sooner.
Warren Straszheim
-----Original Message----- X-from: lkerr-at-mbl.edu [mailto:lkerr-at-mbl.edu] Sent: Friday, August 24, 2007 9:15 AM To: wesaia-at-iastate.edu
I have a question for the List. What is Dark Field STEM? I have a student in our department who wants to use Dark Field STEM to image some fibers and use it to calculate mass per unit length. Mass is directly proportional to the density of the fiber. This is in reference to an article by C. S. Goldsbury in 2000. I need help in understanding how this is not just SEM imaging or inverted STEM imaging and in understanding what programs were used to make the measurements and calculations. Any comments and advice would be welcome.
Thanks, Jeannette
-- Jeannette Taylor, Technologist II IM&MF Cherry L. Emerson Hall, Room E106 Emory University 1515 Dickey Drive Atlanta, Georgia 30322
We're trying to "resurrect" an old Gatan DuoMill and have a problem. Basically, the back guns on both sides have a "dark current" of ~0.5mA when the voltage is set at 6kV and there is no gas flow. When you introduce gas, you can get a beam that looks almost normal. The front guns appear to work correctly but when you select both guns, the back gun appears to be the only one working. Thus, we're trying to identify the source of the "dark current" as that appears to make milling from both sides impossible. Any veterans with experience on these things with suggestions?
Thanks very much for any suggestions.
--John John Chandler, Ph.D. Manager, Electron Microscopy Lab Colorado School of Mines 1500 Illinois Street Golden, CO 80401-1887 jpchandl-at-mines.edu 303.384.2203
==============================Original Headers============================== 6, 19 -- From jpchandl-at-mines.edu Fri Aug 24 17:20:14 2007 6, 19 -- Received: from inception.Mines.EDU (inception.Mines.EDU [138.67.130.4]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7OMKEoc029692 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 17:20:14 -0500 6, 19 -- Received: from emlab1 (emlab1.Mines.EDU [138.67.28.173]) 6, 19 -- by inception.Mines.EDU (8.13.1/8.13.1) with ESMTP id l7OMKDcm004175 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 16:20:13 -0600 6, 19 -- From: "John Chandler" {jpchandl-at-mines.edu} 6, 19 -- To: {microscopy-at-microscopy.com} 6, 19 -- Subject: Gatan DuoMill maintenance question 6, 19 -- Date: Fri, 24 Aug 2007 16:20:11 -0600 6, 19 -- Message-ID: {006e01c7e69c$f0352590$ad1c438a-at-mines.edu} 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; 6, 19 -- charset="us-ascii" 6, 19 -- Content-Transfer-Encoding: 7bit 6, 19 -- X-Mailer: Microsoft Office Outlook 11 6, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 6, 19 -- Thread-Index: AcfmnO/hrBvzAP3gTBGLbtYarWVQiA== ==============================End of - Headers==============================
On Aug 24, 2007, at 2:14 PM, jvtaylo-at-emory.edu wrote:
} I have a question for the List. What is Dark Field STEM? I have a } student in our department who wants to use Dark Field STEM to image } some } fibers and use it to calculate mass per unit length. Mass is directly } proportional to the density of the fiber. This is in reference to an } article by C. S. Goldsbury in 2000. I need help in understanding how } this is not just SEM imaging or inverted STEM imaging and in } understanding what programs were used to make the measurements and } calculations. Any comments and advice would be welcome.
Dear Jeanette, Dark field STEM uses a focussed beam rastered across the specimen to produce an image consisting only of scattered electrons. This is realized by having a detector that does not detect the unscattered beam. For example, a small dot of absorbing material can be put in the objective lens back focal plane where the unscattered beam goes, i.e., in the center of the diffraction pattern, and the unabsorbed electrons can continue on to the detector, or a detector with a hole in the center can be placed in a plane conjugate to the diffraction plane. A practical method that can be used with some specimens is to put the objective aperture off-center to block the unscattered beam. This method produces an image that includes only some of the Fourier components, but if the fiber has the same composition along its length, the proportion of the total scattered beam to that which passes through the aperture and is detected will be constant, so relative measurements of the mass per unit length should be OK. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 5, 22 -- From tivol-at-caltech.edu Fri Aug 24 17:54:49 2007 5, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7OMsmnL009687 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 24 Aug 2007 17:54:48 -0500 5, 22 -- Received: from fire-dog.its.caltech.edu (fire-dog [192.168.1.4]) 5, 22 -- by earth-ox-postvirus (Postfix) with ESMTP id 64D5F1B369 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 24 Aug 2007 15:54:48 -0700 (PDT) 5, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 5, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id D5C4E1B7D3 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 24 Aug 2007 15:54:46 -0700 (PDT) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v752.3) 5, 22 -- In-Reply-To: {200708242114.l7OLEtEx016926-at-ns.microscopy.com} 5, 22 -- References: {200708242114.l7OLEtEx016926-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {2B76635B-88D7-4609-B657-9F1D532BF02F-at-caltech.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 22 -- Subject: Re: [Microscopy] dark field STEM 5, 22 -- Date: Fri, 24 Aug 2007 15:54:46 -0700 5, 22 -- To: microscopy-at-msa.microscopy.com 5, 22 -- X-Mailer: Apple Mail (2.752.3) 5, 22 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.4.5 ==============================End of - Headers==============================
I haven't worked on a DuoMill in a long time, but here are some things that I would look for.
What's the color of the plasma that you can see in the gun? If it is not purple, then you have a vacuum leak. Nitrogen will look pinkish. Nitrogen will ionize much more easily than Ar and if you set it up for both guns, your current will be the one with N2, not the Ar. If it is purple, then you have a short. It might be a carbon track short or a thin film metallization short which will only be seen at a higher voltage. Slowly bring the gun up and see if there is a point where there is a big jump in your current. Your solution there is to take the gun apart and thoroughly clean it, paying particular attention to the ceramics. Any metallization or carburization must be removed or replace the ceramic.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: jpchandl-at-mines.edu [mailto:jpchandl-at-mines.edu] Sent: Friday, August 24, 2007 3:23 PM To: Walck-at-SouthBayTech.com
We're trying to "resurrect" an old Gatan DuoMill and have a problem. Basically, the back guns on both sides have a "dark current" of ~0.5mA when the voltage is set at 6kV and there is no gas flow. When you introduce gas, you can get a beam that looks almost normal. The front guns appear to work correctly but when you select both guns, the back gun appears to be the only one working. Thus, we're trying to identify the source of the "dark current" as that appears to make milling from both sides impossible. Any veterans with experience on these things with suggestions?
Thanks very much for any suggestions.
--John John Chandler, Ph.D. Manager, Electron Microscopy Lab Colorado School of Mines 1500 Illinois Street Golden, CO 80401-1887 jpchandl-at-mines.edu 303.384.2203
==============================Original Headers============================== 6, 19 -- From jpchandl-at-mines.edu Fri Aug 24 17:20:14 2007 6, 19 -- Received: from inception.Mines.EDU (inception.Mines.EDU [138.67.130.4]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7OMKEoc029692 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 17:20:14 -0500 6, 19 -- Received: from emlab1 (emlab1.Mines.EDU [138.67.28.173]) 6, 19 -- by inception.Mines.EDU (8.13.1/8.13.1) with ESMTP id l7OMKDcm004175 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 16:20:13 -0600 6, 19 -- From: "John Chandler" {jpchandl-at-mines.edu} 6, 19 -- To: {microscopy-at-microscopy.com} 6, 19 -- Subject: Gatan DuoMill maintenance question 6, 19 -- Date: Fri, 24 Aug 2007 16:20:11 -0600 6, 19 -- Message-ID: {006e01c7e69c$f0352590$ad1c438a-at-mines.edu} 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; 6, 19 -- charset="us-ascii" 6, 19 -- Content-Transfer-Encoding: 7bit 6, 19 -- X-Mailer: Microsoft Office Outlook 11 6, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 6, 19 -- Thread-Index: AcfmnO/hrBvzAP3gTBGLbtYarWVQiA== ==============================End of - Headers==============================
==============================Original Headers============================== 16, 22 -- From walck-at-southbaytech.com Fri Aug 24 18:01:04 2007 16, 22 -- Received: from flpvm23.prodigy.net (flpvm23.prodigy.net [207.115.20.53]) 16, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7ON1438021336 16, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 18:01:04 -0500 16, 22 -- X-ORBL: [64.169.217.123] 16, 22 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) 16, 22 -- by flpvm23.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id l7ON1CPd014272; 16, 22 -- Fri, 24 Aug 2007 16:01:13 -0700 16, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} 16, 22 -- To: {jpchandl-at-mines.edu} 16, 22 -- Cc: {Microscopy-at-microscopy.com} 16, 22 -- Subject: RE: [Microscopy] Gatan DuoMill maintenance question 16, 22 -- Date: Fri, 24 Aug 2007 16:04:12 -0700 16, 22 -- Message-ID: {010701c7e6a3$16248ba0$7801a8c0-at-dynamicbl8uno3} 16, 22 -- MIME-Version: 1.0 16, 22 -- Content-Type: text/plain; 16, 22 -- charset="us-ascii" 16, 22 -- Content-Transfer-Encoding: 7bit 16, 22 -- X-Mailer: Microsoft Office Outlook 11 16, 22 -- In-Reply-To: {200708242223.l7OMNAmE002775-at-ns.microscopy.com} 16, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 16, 22 -- Thread-Index: AcfmnVvvFWup45IFSIed/FguC8VW/AABLtVA ==============================End of - Headers==============================
I haven't seen a response to this, but you are way off-base on your assumptions.
The wt% and at.% are simply two ways of expressing compositions. The wt% is typically output because materials scientist will almost universally use wt.% to express phase diagrams. The area under an X-ray peak is called the integrated peak intensity (after the background intensity is subtracted.) All of the X-ray microanalysis techniques for bulk samples have to account for atomic number, density, and fluorescence effects. These corrections correct for the production of X-rays as a function of depth (the phi-rho-z curve), absorption of X-rays as a function of depth, fluorescence as a function of depth. Now all of these corrections depend on the composition. The integrated peak intensities for the elements are used to iteratively calculate the compositions. These calculations in the various correction routines are easier to perform using the wt% values for concentrations. I suggest that you look at the Goldstein et al. book for SEM and Microanalysis.
To calculate the at.% of Fe in FeO is easy. It is 1/(1+1) = 0.5 or 50 at.%. For Fe2O3 it is 2/(2+3)= 0.4 or 40%.
To calculate the wt.%, you need to use the atomic weights of the elements.
For Fe in FeO, you have 1*55.85/(1*55.85+1*16.00) = 0.777 or 77.7 wt.%. For Fe2O3 it is (2*55.85)/(2*55.85 + 3*16.00) = 0.699 or 69.9 wt.%.
Once the software finds the wt%, it uses a relatively simple algorithm to calulate the at.%.
You should check any introductory materials science book having a chapter on phase diagrams to see how it is done.
To answer your third question, they are equivalent. When you do an oxide, doing the oxide by ratio, it is easier to do it by at.%.
Now the other question that you asked is again tied up in the stuff that I said above, but there is another thing that you should be aware of and that is the issue of fluorescence yield. There are two competing physical processes that can occur to relieve the excess energy in an atom when a core electron is ejected from that atom. The first is X-ray emission which you are familiar with. The second is Auger electron emission (After Pierre Auger). Auger electron emission is a two electron emission process. Let's take a K shell excitation example. Just as in the emission of a K-alpha X-ray, an electron from the L shell drops into the K shell, but instead of the excess energy coming off in the form of a an X-ray, the excess energy left is enough to ionize another L shell electron. This would be the emission of a KLL Auger electron. Auger electron spectroscopy if a surface analytical technique since the Auger electrons will loose an indeterminate amount of energy if it is emitted within the bulk of the sample and it is just added to the backscattered electron background. X-rays and Auger are competing processes. The X-ray fluorescence yield is the probability of an X-ray being generated when a core shell electron is ionized. The X-ray fluorescence yield is higher for heavier elements than lighter ones. The auger yield is more prevalent for light elements. This is just another natural physical thing that the analyst is fighting against when doing light element X-ray microanalysis.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, August 23, 2007 12:26 AM To: Walck-at-SouthBayTech.com
Dear listers,
I will post soon a summary of all the interesting answers I got for the problem of carbon-free preparation of samples for SEM-EDX (not a lot of solution, though).
Now I face a pretty stupid problem that my pair of neurons can't resolve. When I perform an EDX analysis in SEM (Oxford Instrument's INCA software), I obtain 2 values for each peak: weight % and atom %. I guess that weight % represents the integration of each peak area, whereas atom% represents weight%/atomic weight. Now the 2 values can significantly differ, and thus the element ratio of my samples can also be significantly different. And that is precisely what I want to know!
Now, please allow to ask some questions:
- why is the integration of the peaks in the EDX spectrum called "weight %"? - Does the intensity if the peaks in EDX depend on the atomic weight? In other words, do heavier elements produce more x-rays than lighter elements? It is a hard for me to believe this, because electron shells are the same for light and heavy elements (a K shell is a K shell), however it is the only reason I can see to calculate an atom% value. - Which one of the 2 values to use, in which case and why?
Thank you in advance.
Stephane
____________________________________________________________________________ ________ Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC
==============================Original Headers============================== 12, 19 -- From nizets2-at-yahoo.com Thu Aug 23 02:22:16 2007 12, 19 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 12, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l7N7MGP2010311 12, 19 -- for {microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 02:22:16 -0500 12, 19 -- Received: (qmail 38583 invoked by uid 60001); 23 Aug 2007 07:22:16 -0000 12, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 19 -- s=s1024; d=yahoo.com; 12, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Conten t-Transfer-Encoding:Message-ID; 12, 19 -- b=56SbosFNfmPiKtfWZKu3HWS47mYAYmsgwcM21fBSvygSpvOjQMM1nuHzUEbAq4Jbunzkcn2TVI 8eHOgdpLeI8z4OGEhEfnppqHObYS4VFW1QU8scTot6POOQEPMGa1V50I7hrM1CLvDvIIHR7xA5QJ gZ4GM/AWnM1OxG5rca/Q8=; 12, 19 -- X-YMail-OSG: 07SBxI4VM1nZ7Fk0PON.Y1khqer81hRqg041bYB6G4cwRrmmtCHzE336KWfbXPpfhvZMkGL7oRyx 02fjI.jE0RgTLcEZNUjuIMwnyuvsq0jY_hzdiM2XjMpX.w5hGw-- 12, 19 -- Received: from [80.122.101.102] by web37405.mail.mud.yahoo.com via HTTP; Thu, 23 Aug 2007 00:22:15 PDT 12, 19 -- Date: Thu, 23 Aug 2007 00:22:15 -0700 (PDT) 12, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 12, 19 -- Subject: SEM: wt% or atom% ? 12, 19 -- To: microscopy-at-microscopy.com 12, 19 -- MIME-Version: 1.0 12, 19 -- Content-Type: text/plain; charset=iso-8859-1 12, 19 -- Content-Transfer-Encoding: 8bit 12, 19 -- Message-ID: {963133.38423.qm-at-web37405.mail.mud.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 33, 22 -- From walck-at-southbaytech.com Fri Aug 24 19:42:49 2007 33, 22 -- Received: from flpi102.prodigy.net (flpi102.sbcis.sbc.com [207.115.20.71]) 33, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7P0gntc006837 33, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 19:42:49 -0500 33, 22 -- X-ORBL: [64.169.217.123] 33, 22 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) 33, 22 -- by flpi102.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id l7P0glZI016491; 33, 22 -- Fri, 24 Aug 2007 17:42:47 -0700 33, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} 33, 22 -- To: {nizets2-at-yahoo.com} 33, 22 -- Cc: {Microscopy-at-microscopy.com} 33, 22 -- Subject: RE: [Microscopy] SEM: wt% or atom% ? 33, 22 -- Date: Fri, 24 Aug 2007 17:45:56 -0700 33, 22 -- Message-ID: {011801c7e6b1$4cdb7880$7801a8c0-at-dynamicbl8uno3} 33, 22 -- MIME-Version: 1.0 33, 22 -- Content-Type: text/plain; 33, 22 -- charset="us-ascii" 33, 22 -- Content-Transfer-Encoding: 7bit 33, 22 -- X-Mailer: Microsoft Office Outlook 11 33, 22 -- In-Reply-To: {200708230725.l7N7PvAp015792-at-ns.microscopy.com} 33, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 33, 22 -- Thread-Index: AcflVtqN6hgenZzcSICRUiqE0YBlzQBUh9jw ==============================End of - Headers==============================
I'm in complete agreement with Scott. Also, you can learn a bit more from any textbooks of Modern Physics.
The Answer to your question at the end is "Yes". The K shell of atom A is different of the K shell of atom B as far as their binding energy is concerned. Also given the fact of the existence of ONE vacancy in the K shell, the probability to generate X-ray photons is deferent from Z1 (element one) to Z2 (element two), so are the energy amount of any individual X-rays dictated by characteristic orbit energy differences.
Please note, when you do quantification, you need to take into consideration of a factor called "ZAF", standing for "atomic number", "absorption", and "florescence". For example, a total integral of a lighter element A peak generally carries more weight than the same total integral of a corresponding heavier element B beak assuming the primary energy of the e-beam is sufficient.
Wt% and at% are equivalent (please find that in any General Chemistry book.)
Yes, reading some more books shall help!
Chaoying Ni http://eml.masc.udel.edu
------------ - Does the intensity if the peaks in EDX depend on the atomic weight? In other words, do heavier elements produce more x-rays than lighter elements? It is a hard for me to believe this, because electron shells are the same for light and heavy elements (a K shell is a K shell), however it is the only reason I can see to calculate an atom% value.
-----Original Message----- X-from: walck-at-southbaytech.com [mailto:walck-at-southbaytech.com] Sent: Friday, August 24, 2007 8:45 PM To: cni-at-UDel.Edu
I haven't seen a response to this, but you are way off-base on your assumptions.
The wt% and at.% are simply two ways of expressing compositions. The wt% is typically output because materials scientist will almost universally use wt.% to express phase diagrams. The area under an X-ray peak is called the integrated peak intensity (after the background intensity is subtracted.) All of the X-ray microanalysis techniques for bulk samples have to account for atomic number, density, and fluorescence effects. These corrections correct for the production of X-rays as a function of depth (the phi-rho-z curve), absorption of X-rays as a function of depth, fluorescence as a function of depth. Now all of these corrections depend on the composition. The integrated peak intensities for the elements are used to iteratively calculate the compositions. These calculations in the various correction routines are easier to perform using the wt% values for concentrations. I suggest that you look at the Goldstein et al. book for SEM and Microanalysis.
To calculate the at.% of Fe in FeO is easy. It is 1/(1+1) = 0.5 or 50 at.%. For Fe2O3 it is 2/(2+3)= 0.4 or 40%.
To calculate the wt.%, you need to use the atomic weights of the elements.
For Fe in FeO, you have 1*55.85/(1*55.85+1*16.00) = 0.777 or 77.7 wt.%. For Fe2O3 it is (2*55.85)/(2*55.85 + 3*16.00) = 0.699 or 69.9 wt.%.
Once the software finds the wt%, it uses a relatively simple algorithm to calulate the at.%.
You should check any introductory materials science book having a chapter on phase diagrams to see how it is done.
To answer your third question, they are equivalent. When you do an oxide, doing the oxide by ratio, it is easier to do it by at.%.
Now the other question that you asked is again tied up in the stuff that I said above, but there is another thing that you should be aware of and that is the issue of fluorescence yield. There are two competing physical processes that can occur to relieve the excess energy in an atom when a core electron is ejected from that atom. The first is X-ray emission which you are familiar with. The second is Auger electron emission (After Pierre Auger). Auger electron emission is a two electron emission process. Let's take a K shell excitation example. Just as in the emission of a K-alpha X-ray, an electron from the L shell drops into the K shell, but instead of the excess energy coming off in the form of a an X-ray, the excess energy left is enough to ionize another L shell electron. This would be the emission of a KLL Auger electron. Auger electron spectroscopy if a surface analytical technique since the Auger electrons will loose an indeterminate amount of energy if it is emitted within the bulk of the sample and it is just added to the backscattered electron background. X-rays and Auger are competing processes. The X-ray fluorescence yield is the probability of an X-ray being generated when a core shell electron is ionized. The X-ray fluorescence yield is higher for heavier elements than lighter ones. The auger yield is more prevalent for light elements. This is just another natural physical thing that the analyst is fighting against when doing light element X-ray microanalysis.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:c] Sent: Thursday, August 23, 2007 12:26 AM To: Walck-at-SouthBayTech.com
Dear listers,
I will post soon a summary of all the interesting answers I got for the problem of carbon-free preparation of samples for SEM-EDX (not a lot of solution, though).
Now I face a pretty stupid problem that my pair of neurons can't resolve. When I perform an EDX analysis in SEM (Oxford Instrument's INCA software), I obtain 2 values for each peak: weight % and atom %. I guess that weight % represents the integration of each peak area, whereas atom% represents weight%/atomic weight. Now the 2 values can significantly differ, and thus the element ratio of my samples can also be significantly different. And that is precisely what I want to know!
Now, please allow to ask some questions:
- why is the integration of the peaks in the EDX spectrum called "weight %"? - Does the intensity if the peaks in EDX depend on the atomic weight? In other words, do heavier elements produce more x-rays than lighter elements? It is a hard for me to believe this, because electron shells are the same for light and heavy elements (a K shell is a K shell), however it is the only reason I can see to calculate an atom% value. - Which one of the 2 values to use, in which case and why?
Thank you in advance.
Stephane
________________________________________________________________________ ____ ________ Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC
==============================Original Headers============================== 12, 19 -- From nizets2-at-yahoo.com Thu Aug 23 02:22:16 2007 12, 19 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 12, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l7N7MGP2010311 12, 19 -- for {microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 02:22:16 -0500 12, 19 -- Received: (qmail 38583 invoked by uid 60001); 23 Aug 2007 07:22:16 -0000 12, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 19 -- s=s1024; d=yahoo.com; 12, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Co nten t-Transfer-Encoding:Message-ID; 12, 19 -- b=56SbosFNfmPiKtfWZKu3HWS47mYAYmsgwcM21fBSvygSpvOjQMM1nuHzUEbAq4Jbunzkcn 2TVI 8eHOgdpLeI8z4OGEhEfnppqHObYS4VFW1QU8scTot6POOQEPMGa1V50I7hrM1CLvDvIIHR7x A5QJ gZ4GM/AWnM1OxG5rca/Q8=; 12, 19 -- X-YMail-OSG: 07SBxI4VM1nZ7Fk0PON.Y1khqer81hRqg041bYB6G4cwRrmmtCHzE336KWfbXPpfhvZMkGL7 oRyx 02fjI.jE0RgTLcEZNUjuIMwnyuvsq0jY_hzdiM2XjMpX.w5hGw-- 12, 19 -- Received: from [80.122.101.102] by web37405.mail.mud.yahoo.com via HTTP; Thu, 23 Aug 2007 00:22:15 PDT 12, 19 -- Date: Thu, 23 Aug 2007 00:22:15 -0700 (PDT) 12, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 12, 19 -- Subject: SEM: wt% or atom% ? 12, 19 -- To: microscopy-at-microscopy.com 12, 19 -- MIME-Version: 1.0 12, 19 -- Content-Type: text/plain; charset=iso-8859-1 12, 19 -- Content-Transfer-Encoding: 8bit 12, 19 -- Message-ID: {963133.38423.qm-at-web37405.mail.mud.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 33, 22 -- From walck-at-southbaytech.com Fri Aug 24 19:42:49 2007 33, 22 -- Received: from flpi102.prodigy.net (flpi102.sbcis.sbc.com [207.115.20.71]) 33, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7P0gntc006837 33, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 19:42:49 -0500 33, 22 -- X-ORBL: [64.169.217.123] 33, 22 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) 33, 22 -- by flpi102.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id l7P0glZI016491; 33, 22 -- Fri, 24 Aug 2007 17:42:47 -0700 33, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} 33, 22 -- To: {nizets2-at-yahoo.com} 33, 22 -- Cc: {Microscopy-at-microscopy.com} 33, 22 -- Subject: RE: [Microscopy] SEM: wt% or atom% ? 33, 22 -- Date: Fri, 24 Aug 2007 17:45:56 -0700 33, 22 -- Message-ID: {011801c7e6b1$4cdb7880$7801a8c0-at-dynamicbl8uno3} 33, 22 -- MIME-Version: 1.0 33, 22 -- Content-Type: text/plain; 33, 22 -- charset="us-ascii" 33, 22 -- Content-Transfer-Encoding: 7bit 33, 22 -- X-Mailer: Microsoft Office Outlook 11 33, 22 -- In-Reply-To: {200708230725.l7N7PvAp015792-at-ns.microscopy.com} 33, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 33, 22 -- Thread-Index: AcflVtqN6hgenZzcSICRUiqE0YBlzQBUh9jw ==============================End of - Headers==============================
==============================Original Headers============================== 51, 24 -- From cni-at-udel.edu Fri Aug 24 22:13:13 2007 51, 24 -- Received: from md2.nss.udel.edu (md2.nss.udel.edu [128.175.1.12]) 51, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7P3DDNZ013440 51, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 22:13:13 -0500 51, 24 -- Received: from cni (c-68-82-7-59.hsd1.de.comcast.net [68.82.7.59]) 51, 24 -- by md2.nss.udel.edu (MOS 3.8.4-GA) 51, 24 -- with ESMTP id ESX43326 (AUTH via LOGINBEFORESMTP); 51, 24 -- Fri, 24 Aug 2007 23:13:12 -0400 (EDT) 51, 24 -- From: "CNi" {cni-at-udel.edu} 51, 24 -- To: {nizets2-at-yahoo.com} 51, 24 -- Cc: {Microscopy-at-microscopy.com} 51, 24 -- Subject: RE: [Microscopy] RE: SEM: wt% or atom% ? 51, 24 -- Date: Fri, 24 Aug 2007 23:13:15 -0400 51, 24 -- Message-ID: {000101c7e6c5$e0881930$6401a8c0-at-cni} 51, 24 -- MIME-Version: 1.0 51, 24 -- Content-Type: text/plain; 51, 24 -- charset="us-ascii" 51, 24 -- Content-Transfer-Encoding: 7bit 51, 24 -- X-Priority: 3 (Normal) 51, 24 -- X-MSMail-Priority: Normal 51, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 51, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 51, 24 -- Importance: Normal 51, 24 -- In-Reply-To: {200708250044.l7P0iXCQ009839-at-ns.microscopy.com} ==============================End of - Headers==============================
I meant given wt%'s of elements A, B, C., their at%'s can be calculated, or vice versa, of course, unless you would definitely prefer a specific stoichiometry for some elements among the A, B, C.. -cni
-----Original Message----- X-from: Straszheim, Warren E [M S E] [mailto:wesaia-at-iastate.edu] Sent: Saturday, August 25, 2007 12:17 AM To: cni-at-UDel.Edu
Hi Stephane,
I'm in complete agreement with Scott. Also, you can learn a bit more from any textbooks of Modern Physics.
The Answer to your question at the end is "Yes". The K shell of atom A is different of the K shell of atom B as far as their binding energy is concerned. Also given the fact of the existence of ONE vacancy in the K shell, the probability to generate X-ray photons is deferent from Z1 (element one) to Z2 (element two), so are the energy amount of any individual X-rays dictated by characteristic orbit energy differences.
Please note, when you do quantification, you need to take into consideration of a factor called "ZAF", standing for "atomic number", "absorption", and "florescence". For example, a total integral of a lighter element A peak generally carries more weight than the same total integral of a corresponding heavier element B beak assuming the primary energy of the e-beam is sufficient.
Wt% and at% are equivalent (please find that in any General Chemistry book.)
Yes, reading some more books shall help!
Chaoying Ni http://eml.masc.udel.edu
------------ - Does the intensity if the peaks in EDX depend on the atomic weight? In other words, do heavier elements produce more x-rays than lighter elements? It is a hard for me to believe this, because electron shells are the same for light and heavy elements (a K shell is a K shell), however it is the only reason I can see to calculate an atom% value.
-----Original Message----- X-from: walck-at-southbaytech.com [mailto:walck-at-southbaytech.com] Sent: Friday, August 24, 2007 8:45 PM To: cni-at-UDel.Edu
I haven't seen a response to this, but you are way off-base on your assumptions.
The wt% and at.% are simply two ways of expressing compositions. The wt% is typically output because materials scientist will almost universally use wt.% to express phase diagrams. The area under an X-ray peak is called the integrated peak intensity (after the background intensity is subtracted.) All of the X-ray microanalysis techniques for bulk samples have to account for atomic number, density, and fluorescence effects. These corrections correct for the production of X-rays as a function of depth (the phi-rho-z curve), absorption of X-rays as a function of depth, fluorescence as a function of depth. Now all of these corrections depend on the composition. The integrated peak intensities for the elements are used to iteratively calculate the compositions. These calculations in the various correction routines are easier to perform using the wt% values for concentrations. I suggest that you look at the Goldstein et al. book for SEM and Microanalysis.
To calculate the at.% of Fe in FeO is easy. It is 1/(1+1) = 0.5 or 50 at.%. For Fe2O3 it is 2/(2+3)= 0.4 or 40%.
To calculate the wt.%, you need to use the atomic weights of the elements.
For Fe in FeO, you have 1*55.85/(1*55.85+1*16.00) = 0.777 or 77.7 wt.%. For Fe2O3 it is (2*55.85)/(2*55.85 + 3*16.00) = 0.699 or 69.9 wt.%.
Once the software finds the wt%, it uses a relatively simple algorithm to calulate the at.%.
You should check any introductory materials science book having a chapter on phase diagrams to see how it is done.
To answer your third question, they are equivalent. When you do an oxide, doing the oxide by ratio, it is easier to do it by at.%.
Now the other question that you asked is again tied up in the stuff that I said above, but there is another thing that you should be aware of and that is the issue of fluorescence yield. There are two competing physical processes that can occur to relieve the excess energy in an atom when a core electron is ejected from that atom. The first is X-ray emission which you are familiar with. The second is Auger electron emission (After Pierre Auger). Auger electron emission is a two electron emission process. Let's take a K shell excitation example. Just as in the emission of a K-alpha X-ray, an electron from the L shell drops into the K shell, but instead of the excess energy coming off in the form of a an X-ray, the excess energy left is enough to ionize another L shell electron. This would be the emission of a KLL Auger electron. Auger electron spectroscopy if a surface analytical technique since the Auger electrons will loose an indeterminate amount of energy if it is emitted within the bulk of the sample and it is just added to the backscattered electron background. X-rays and Auger are competing processes. The X-ray fluorescence yield is the probability of an X-ray being generated when a core shell electron is ionized. The X-ray fluorescence yield is higher for heavier elements than lighter ones. The auger yield is more prevalent for light elements. This is just another natural physical thing that the analyst is fighting against when doing light element X-ray microanalysis.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:c] Sent: Thursday, August 23, 2007 12:26 AM To: Walck-at-SouthBayTech.com
Dear listers,
I will post soon a summary of all the interesting answers I got for the problem of carbon-free preparation of samples for SEM-EDX (not a lot of solution, though).
Now I face a pretty stupid problem that my pair of neurons can't resolve. When I perform an EDX analysis in SEM (Oxford Instrument's INCA software), I obtain 2 values for each peak: weight % and atom %. I guess that weight % represents the integration of each peak area, whereas atom% represents weight%/atomic weight. Now the 2 values can significantly differ, and thus the element ratio of my samples can also be significantly different. And that is precisely what I want to know!
Now, please allow to ask some questions:
- why is the integration of the peaks in the EDX spectrum called "weight %"? - Does the intensity if the peaks in EDX depend on the atomic weight? In other words, do heavier elements produce more x-rays than lighter elements? It is a hard for me to believe this, because electron shells are the same for light and heavy elements (a K shell is a K shell), however it is the only reason I can see to calculate an atom% value. - Which one of the 2 values to use, in which case and why?
Thank you in advance.
Stephane
________________________________________________________________________ ____ ________ Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC
==============================Original Headers============================== 12, 19 -- From nizets2-at-yahoo.com Thu Aug 23 02:22:16 2007 12, 19 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 12, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l7N7MGP2010311 12, 19 -- for {microscopy-at-microscopy.com} ; Thu, 23 Aug 2007 02:22:16 -0500 12, 19 -- Received: (qmail 38583 invoked by uid 60001); 23 Aug 2007 07:22:16 -0000 12, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 19 -- s=s1024; d=yahoo.com; 12, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Co nten t-Transfer-Encoding:Message-ID; 12, 19 -- b=56SbosFNfmPiKtfWZKu3HWS47mYAYmsgwcM21fBSvygSpvOjQMM1nuHzUEbAq4Jbunzkcn 2TVI 8eHOgdpLeI8z4OGEhEfnppqHObYS4VFW1QU8scTot6POOQEPMGa1V50I7hrM1CLvDvIIHR7x A5QJ gZ4GM/AWnM1OxG5rca/Q8=; 12, 19 -- X-YMail-OSG: 07SBxI4VM1nZ7Fk0PON.Y1khqer81hRqg041bYB6G4cwRrmmtCHzE336KWfbXPpfhvZMkGL7 oRyx 02fjI.jE0RgTLcEZNUjuIMwnyuvsq0jY_hzdiM2XjMpX.w5hGw-- 12, 19 -- Received: from [80.122.101.102] by web37405.mail.mud.yahoo.com via HTTP; Thu, 23 Aug 2007 00:22:15 PDT 12, 19 -- Date: Thu, 23 Aug 2007 00:22:15 -0700 (PDT) 12, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 12, 19 -- Subject: SEM: wt% or atom% ? 12, 19 -- To: microscopy-at-microscopy.com 12, 19 -- MIME-Version: 1.0 12, 19 -- Content-Type: text/plain; charset=iso-8859-1 12, 19 -- Content-Transfer-Encoding: 8bit 12, 19 -- Message-ID: {963133.38423.qm-at-web37405.mail.mud.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 33, 22 -- From walck-at-southbaytech.com Fri Aug 24 19:42:49 2007 33, 22 -- Received: from flpi102.prodigy.net (flpi102.sbcis.sbc.com [207.115.20.71]) 33, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7P0gntc006837 33, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 19:42:49 -0500 33, 22 -- X-ORBL: [64.169.217.123] 33, 22 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) 33, 22 -- by flpi102.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id l7P0glZI016491; 33, 22 -- Fri, 24 Aug 2007 17:42:47 -0700 33, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} 33, 22 -- To: {nizets2-at-yahoo.com} 33, 22 -- Cc: {Microscopy-at-microscopy.com} 33, 22 -- Subject: RE: [Microscopy] SEM: wt% or atom% ? 33, 22 -- Date: Fri, 24 Aug 2007 17:45:56 -0700 33, 22 -- Message-ID: {011801c7e6b1$4cdb7880$7801a8c0-at-dynamicbl8uno3} 33, 22 -- MIME-Version: 1.0 33, 22 -- Content-Type: text/plain; 33, 22 -- charset="us-ascii" 33, 22 -- Content-Transfer-Encoding: 7bit 33, 22 -- X-Mailer: Microsoft Office Outlook 11 33, 22 -- In-Reply-To: {200708230725.l7N7PvAp015792-at-ns.microscopy.com} 33, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 33, 22 -- Thread-Index: AcflVtqN6hgenZzcSICRUiqE0YBlzQBUh9jw ==============================End of - Headers==============================
==============================Original Headers============================== 51, 24 -- From cni-at-udel.edu Fri Aug 24 22:13:13 2007 51, 24 -- Received: from md2.nss.udel.edu (md2.nss.udel.edu [128.175.1.12]) 51, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7P3DDNZ013440 51, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 22:13:13 -0500 51, 24 -- Received: from cni (c-68-82-7-59.hsd1.de.comcast.net [68.82.7.59]) 51, 24 -- by md2.nss.udel.edu (MOS 3.8.4-GA) 51, 24 -- with ESMTP id ESX43326 (AUTH via LOGINBEFORESMTP); 51, 24 -- Fri, 24 Aug 2007 23:13:12 -0400 (EDT) 51, 24 -- From: "CNi" {cni-at-udel.edu} 51, 24 -- To: {nizets2-at-yahoo.com} 51, 24 -- Cc: {Microscopy-at-microscopy.com} 51, 24 -- Subject: RE: [Microscopy] RE: SEM: wt% or atom% ? 51, 24 -- Date: Fri, 24 Aug 2007 23:13:15 -0400 51, 24 -- Message-ID: {000101c7e6c5$e0881930$6401a8c0-at-cni} 51, 24 -- MIME-Version: 1.0 51, 24 -- Content-Type: text/plain; 51, 24 -- charset="us-ascii" 51, 24 -- Content-Transfer-Encoding: 7bit 51, 24 -- X-Priority: 3 (Normal) 51, 24 -- X-MSMail-Priority: Normal 51, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 51, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 51, 24 -- Importance: Normal 51, 24 -- In-Reply-To: {200708250044.l7P0iXCQ009839-at-ns.microscopy.com} ==============================End of - Headers==============================
==============================Original Headers============================== 66, 25 -- From cni-at-udel.edu Sat Aug 25 06:10:46 2007 66, 25 -- Received: from md1.nss.udel.edu (md1.nss.udel.edu [128.175.1.11]) 66, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7PBAjqb021779 66, 25 -- for {Microscopy-at-microscopy.com} ; Sat, 25 Aug 2007 06:10:46 -0500 66, 25 -- Received: from cni (c-68-82-7-59.hsd1.de.comcast.net [68.82.7.59]) 66, 25 -- by md1.nss.udel.edu (MOS 3.8.4-GA) 66, 25 -- with ESMTP id DPO34747 (AUTH via LOGINBEFORESMTP); 66, 25 -- Sat, 25 Aug 2007 07:10:45 -0400 (EDT) 66, 25 -- From: "CNi" {cni-at-udel.edu} 66, 25 -- To: "'Straszheim, Warren E [M S E]'" {wesaia-at-iastate.edu} 66, 25 -- Cc: {Microscopy-at-microscopy.com} 66, 25 -- Subject: RE: [Microscopy] SEM: wt% or atom% ? 66, 25 -- Date: Sat, 25 Aug 2007 07:10:48 -0400 66, 25 -- Message-ID: {000b01c7e708$97110c60$6401a8c0-at-cni} 66, 25 -- MIME-Version: 1.0 66, 25 -- Content-Type: text/plain; 66, 25 -- charset="iso-8859-1" 66, 25 -- X-Priority: 3 (Normal) 66, 25 -- X-MSMail-Priority: Normal 66, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 66, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 66, 25 -- Importance: Normal 66, 25 -- In-Reply-To: {16A330AC32056A40B32842EC4BB8D727013B102A-at-maire.eng.iastate.edu} 66, 25 -- Content-Transfer-Encoding: 8bit 66, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7PBAjqb021779 ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (jrichards-at-macslab.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, August 25, 2007 at 11:29:01 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both jrichards-at-macslab.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: jrichards-at-macslab.com Name: Jim Richards
Organization: San Juan Delta College
Education: Undergraduate College
Location: Stockton, CA
Title: Electron diffraction
Question: When looking at a single crystal and obtaining a diffraction pattern (SAED) obviously the electron beam enters the crystal at some angles relative to the crystal's lattice. The d spacing changes as a result of tilting. From this one can determine the zone axis. Therefore one must tilt (single or double) to get a specific zone axis or even get a diffraction pattern that will yield d spacing for a zone axis. How then do people claim that the d spacing will always be the same regardless of the tilt of the sample or how the sample is positioned in the objective lens on the sample holder? Example, we were looking at a gunerite crystal and it was claimed that the d spacing was X and therefore this was enough evidence to conclude that the crystal was gunerite. I don't understand.
Don't you have to find a zone axis by determine the d spacing and the interplaner angle then consult literature or a computer program to determine what zone axis you have and if the spacing matches then you might have that mineral?
If you have a turbo on that DuoMill, then you are best off swapping front/back parts until you isolate the problem.
If you have a diff-pump, then you need to just be more patient.
This should save you the cost of replacinng every oring, ceramic, octa-tube, gun casing, etc.....
JQuinn
} From mail-at-ns.microscopy.com Fri Aug 24 18:21:37 2007 } Date: Fri, 24 Aug 2007 17:20:55 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: jpchandl-at-mines.edu } Reply-to: jpchandl-at-mines.edu } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] Gatan DuoMill maintenance question } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We're trying to "resurrect" an old Gatan DuoMill and have a problem. } Basically, the back guns on both sides have a "dark current" of ~0.5mA when } the voltage is set at 6kV and there is no gas flow. When you introduce gas, } you can get a beam that looks almost normal. The front guns appear to work } correctly but when you select both guns, the back gun appears to be the only } one working. Thus, we're trying to identify the source of the "dark } current" as that appears to make milling from both sides impossible. Any } veterans with experience on these things with suggestions? } } Thanks very much for any suggestions. } } --John } John Chandler, Ph.D. } Manager, Electron Microscopy Lab } Colorado School of Mines } 1500 Illinois Street } Golden, CO 80401-1887 } jpchandl-at-mines.edu } 303.384.2203 } } } } } ==============================Original Headers============================== } 6, 19 -- From jpchandl-at-mines.edu Fri Aug 24 17:20:14 2007 } 6, 19 -- Received: from inception.Mines.EDU (inception.Mines.EDU [138.67.130.4]) } 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7OMKEoc029692 } 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 17:20:14 -0500 } 6, 19 -- Received: from emlab1 (emlab1.Mines.EDU [138.67.28.173]) } 6, 19 -- by inception.Mines.EDU (8.13.1/8.13.1) with ESMTP id l7OMKDcm004175 } 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 24 Aug 2007 16:20:13 -0600 } 6, 19 -- From: "John Chandler" {jpchandl-at-mines.edu} } 6, 19 -- To: {microscopy-at-microscopy.com} } 6, 19 -- Subject: Gatan DuoMill maintenance question } 6, 19 -- Date: Fri, 24 Aug 2007 16:20:11 -0600 } 6, 19 -- Message-ID: {006e01c7e69c$f0352590$ad1c438a-at-mines.edu} } 6, 19 -- MIME-Version: 1.0 } 6, 19 -- Content-Type: text/plain; } 6, 19 -- charset="us-ascii" } 6, 19 -- Content-Transfer-Encoding: 7bit } 6, 19 -- X-Mailer: Microsoft Office Outlook 11 } 6, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 } 6, 19 -- Thread-Index: AcfmnO/hrBvzAP3gTBGLbtYarWVQiA== } ==============================End of - Headers============================== }
==============================Original Headers============================== 10, 12 -- From jquinn-at-www.matscieng.sunysb.edu Sat Aug 25 12:21:51 2007 10, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 10, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7PHLpIv021126 10, 12 -- for {microscopy-at-microscopy.com} ; Sat, 25 Aug 2007 12:21:51 -0500 10, 12 -- Received: (from jquinn-at-localhost) 10, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id l7PHMN630679 10, 12 -- for microscopy-at-microscopy.com; Sat, 25 Aug 2007 13:22:23 -0400 10, 12 -- Date: Sat, 25 Aug 2007 13:22:23 -0400 10, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 10, 12 -- Message-Id: {200708251722.l7PHMN630679-at-www.matscieng.sunysb.edu} 10, 12 -- To: microscopy-at-microscopy.com 10, 12 -- Subject: re: Gatan DuoMill maintenance question ==============================End of - Headers==============================
Our solution to the common poor section contrasting of fast frozen, freeze substituted, and low temperature HM20 Lowicryl embedded material was first presented (on microtubule barrels of isolated centrosomes) in the (completely obscure) paper… Histochemical Journal 27, 240-246 (1995).
Treating Lowicryl HM20 sections like fresh tissue (= post sectioning fixation) can produce sections with contrast and morphological preservation equivalent to epon sections using standard TEM stains.
This technique was then applied to whole mammalian cells low temperature processed into HM20, resulting in “epon-like’ contrast of whole cells with well defined membranes and microtubules… Current Biology 5 (12): 1384–1393 (1995).
The technique applied to isolated virus samples… Journal of Virology 73(3): 1931–1940 (1999).
The technique has also been applied to low temperature processed yeast, bacteria, and plant cells with equally good results (no published images).
“Post-Sectioning fixation” (after sections immunolabelled and in final water rinse) 1) freshly prepared and filtered 2% tannic acid, pH 7.2 for 10 minutes 2) rinsed three times in 1.0% sodium sulphate, 5 seconds each 3) distilled water rinses, three times for 1 minute each 4) 2% glutaraldehyde in water, 10 minutes 5) distilled water rinses, three times for 1 minute each 6) 1.0% osmium tetroxide in water, 10 minutes 7) distilled water rinses, 4 times for 1 minute each 8) 5% uranyl acetate in 50% ethanol for 10 minutes 9) distilled water rinses, three times for 1 minute each 10) repeat steps 1) – 3) = optional (only if you want to enhance microtubules to the maximum). 11) 5.0% lead citrate in water, 4 minutes 12) distilled water rinses, four times for 1 minute each
I have only used HM20 and so do not know if this will work with the polar Lowicryl K4M.
Material lightly aldehyde fixed and processed using the Progressive Lowering Temperature method often benefits from the post-sectioning fixation for section contrast as well.
Cheers
B
---------------------------------------------------------------------------- } } Kenneth- } I have never tried this, but I would be surpised if it would work. } Lowicrl resin is itself quite reactive, and I would expect it to have } reacted with a lot of the primary and secondary amines that OsO4 } reacts with. This may be worth a try though. It has never been } reported, to my knowledge, so it would be new knowledge. } Carol } }
} } Email: kdunnerj-at-mdanderson.org } } Name: Kenneth Dunner Jr } } } } Organization: MD Anderson Cancer Center } } } } Title-Subject: [Filtered] Osmium tetroxide on Lowicryl Sections } } } } Question: A colleague I know wants to know has anyone used osmium } } tetroxide on post embedded immunogold labeled Lowicryl sections to } } make microtubules or other membranes stand out? } } } } ---------------------------------------------------------------------------
********************************************************************** Brent Gowen Electron Microscopy Laboratory Department of Biology University of Victoria P.O. Box 3020 STN CSC Victoria, BC, Canada V8W 3N5 Tel: (250)-721-7132 http://web.uvic.ca/em-lab/
==============================Original Headers============================== 20, 27 -- From bgowen-at-uvic.ca Sat Aug 25 12:56:07 2007 20, 27 -- Received: from castle.comp.uvic.ca (castle.comp.uvic.ca [142.104.5.97]) 20, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7PHu69Y001140 20, 27 -- for {microscopy-at-microscopy.com} ; Sat, 25 Aug 2007 12:56:07 -0500 20, 27 -- Received: from wm3.uvic.ca (godwit.comp.uvic.ca [142.104.148.224]) 20, 27 -- by castle.comp.uvic.ca (8.13.8/8.13.8) with ESMTP id l7PHu6Me6316124 20, 27 -- for {microscopy-at-microscopy.com} ; Sat, 25 Aug 2007 10:56:06 -0700 20, 27 -- Received: from 142.104.193.193 (proxying for 24.68.197.23) 20, 27 -- (SquirrelMail authenticated user bgowen) 20, 27 -- by wm3.uvic.ca with HTTP; 20, 27 -- Sat, 25 Aug 2007 10:56:06 -0700 (PDT) 20, 27 -- Message-ID: {19474.142.104.193.193.1188064566.squirrel-at-wm3.uvic.ca} 20, 27 -- In-Reply-To: {200708241520.l7OFKi5a017579-at-ns.microscopy.com} 20, 27 -- References: {200708241520.l7OFKi5a017579-at-ns.microscopy.com} 20, 27 -- Date: Sat, 25 Aug 2007 10:56:06 -0700 (PDT) 20, 27 -- Subject: Re: viaWWW: Osmium tetroxide on Lowicryl Sections 20, 27 -- From: "bgowen" {bgowen-at-uvic.ca} 20, 27 -- To: microscopy-at-microscopy.com 20, 27 -- User-Agent: SquirrelMail/1.4.9a 20, 27 -- MIME-Version: 1.0 20, 27 -- Content-Type: text/plain;charset=iso-8859-1 20, 27 -- Content-Transfer-Encoding: 8bit 20, 27 -- X-Priority: 3 (Normal) 20, 27 -- Importance: Normal 20, 27 -- X-UVic-Virus-Scanned: OK - Passed virus scan by Sophos (sophie) on castle 20, 27 -- X-UVic-Spam-Scan: castle.comp.uvic.ca Not_scanned_LOCAL 20, 27 -- X-Scanned-By: MIMEDefang 2.33 (www . roaringpenguin . com / mimedefang) ==============================End of - Headers==============================
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
As you tilt a crystal, different lattice plane sets diffract. So, the d-spacings of lattice planes is 'fixed', it's just that at different crystal tilts, different d-spacings appear in the diffraction pattern.
Having said that, unless you are very careful, identifying phases on the basis of simple selected area diffraction patterns is asking for trouble bigtime:
1. SAED can only reliably 'select' a known area down to regions of ~1 um. If you do SAED on smaller areas, aberations in the imaging lens mean that higher order reflections come from regions different from the lower order reflections. So, SAED with multi-phase samples and small crystal will be asking for the wrong answer.
2. The calibration of any TEM camera length is only accurate to ~5%, unless you use an internal calibration with known d-spacings.
3. Taking into consideration the above, you should really do exactly as you say - find a zone axis, identify the axis and measure interplanar spacings/angles. Even so, you need to keep in mind that the accuracy of measurement is ~5%, so you then need to be able to exclude other possible phases with similar crystal structures and d-spacings.
4. EDS analysis is a big help - knowing the approximate composition allows you to exclude many possible phases quickly.
5. Personally, I would never use SAED - small probe diffraction methods are much better, allowing you to reliably do electron diffraction from small regions. If you use convergent beam electron diffraction, the pattern symmetries allow you to identify the crystallography very reliably.
-- Larry Stoter
PLEASE NOTE - Agressive SPAM filtering is employed. If you don't get a reply to e-mails, try again, avoiding anything in the subject or body which might trigger filtering. If you are in the address book, you should get through. If you aren't, then there's a chance your e-mail will never be seen.
==============================Original Headers============================== 10, 15 -- From larry-at-cymru666.plus.com Sun Aug 26 03:35:21 2007 10, 15 -- Received: from pih-relay04.plus.net (pih-relay04.plus.net [212.159.14.131]) 10, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7Q8ZKYe020908 10, 15 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sun, 26 Aug 2007 03:35:21 -0500 10, 15 -- Received: from [87.112.82.84] (helo=[192.168.1.2]) 10, 15 -- by pih-relay04.plus.net with esmtp (Exim) id 1IPDaZ-0000Si-61; Sun, 26 Aug 2007 09:35:19 +0100 10, 15 -- Mime-Version: 1.0 10, 15 -- Message-Id: {p06240800c2f6df7074a2-at-[192.168.1.2]} 10, 15 -- In-Reply-To: {200708251650.l7PGo3IA019505-at-ns.microscopy.com} 10, 15 -- References: {200708251650.l7PGo3IA019505-at-ns.microscopy.com} 10, 15 -- Date: Sun, 26 Aug 2007 08:56:50 +0100 10, 15 -- To: jrichards-at-macslab.com, Microscopy-at-MSA.Microscopy.Com 10, 15 -- From: Larry Stoter {larry-at-cymru666.plus.com} 10, 15 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: Electron diffraction 10, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I do not work with confocals so am unfamiliar with many of the staining procedures used for plant as well as animal samples. I would like to know if it is necessary or even desirable to have a fume hood in the same room with a high-end confocal.
Also, providing there are the reagents that may require a fume hood, can this need be satisfied with one of the movable hoods that use filters such as are used in many light microscope/histology labs?
Does anyone have a source they can recommend for such hoods?
Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 7, 21 -- From dsherman-at-purdue.edu Sun Aug 26 16:39:58 2007 7, 21 -- Received: from 1061exfe01a.itap.purdue.edu (1061exfe01a.itap.purdue.edu [128.210.1.8]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7QLdwUb031162 7, 21 -- for {microscopy-at-microscopy.com} ; Sun, 26 Aug 2007 16:39:58 -0500 7, 21 -- Received: from exchange.purdue.edu ([172.21.6.24]) by 1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 21 -- Sun, 26 Aug 2007 17:40:00 -0400 7, 21 -- Received: from 74.140.109.252 ([74.140.109.252]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 7, 21 -- Sun, 26 Aug 2007 21:40:00 +0000 7, 21 -- User-Agent: Microsoft-Entourage/11.3.6.070618 7, 21 -- Date: Sun, 26 Aug 2007 17:39:55 -0400 7, 21 -- Subject: Fume hood for confocal 7, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 7, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 7, 21 -- Message-ID: {C2F76B6B.10D6C%dsherman-at-purdue.edu} 7, 21 -- Thread-Topic: Fume hood for confocal 7, 21 -- Thread-Index: AcfoKaQ74wa4PFQcEdy95gAbY5Fdpg== 7, 21 -- Mime-version: 1.0 7, 21 -- Content-type: text/plain; 7, 21 -- charset="US-ASCII" 7, 21 -- Content-transfer-encoding: 7bit 7, 21 -- X-OriginalArrivalTime: 26 Aug 2007 21:40:00.0820 (UTC) FILETIME=[A7B35740:01C7E829] ==============================End of - Headers==============================
I have 2 confocals (and about to order my 3rd) and very happy that I don't have a hood in the same room. the noise and possibly vibration would be annoying. some older confocals required a blower motor to pull heat away from the lasers but came with a small fan that blew the air via a dryer hose into the plenum (i.e., space above the ceiling). the staining for confocal is no different than any other immunostaining procedure. we have a hood in a prep room next to our 2 confocals and our clients never use it. one generally prepares the tissue offsite and brings it to the confocal room. Good luck, Tom
At 04:42 PM 08/26/07, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
With respect to at% and wt% you may be able to convert between them using the gravimetric factors. However if you calculate your values based on at% and wt% then you will end up with different values because of the assumption about the unmeasured O - one calculation will normalize the ZAF calculations on the bases of stoichiometry the other on the O difference these may converge to different values for none ideal samples Stuart
================================================ Stuart McClure CSIRO Land and Water Post: P.B#2, Glen Osmond, SA, Australia, 5064 Street: Waite Rd, Urrbrae, SA, Australia, 5064 Site: via Gate #5, Building Taylor-3A, Room 218 International [ x ] National ( x ) Tel-W: [ 618 ] ( 08 ) 8303 8484 Fax-W: [ 618 ] ( 08 ) 8303 8550 Tel-H: [ 618 ] ( 08 ) 8297 3452 Mob-H: [ 61 ] ( 0 ) 428 100 796 Email1: stuart.mcclure-at-csiro.au Email2: stuart.mcclure-at-gmail.com Email3: stuart.mcclure-at-adelaide.edu.au ================================================
==============================Original Headers============================== 5, 28 -- From Stuart.McClure-at-csiro.au Sun Aug 26 21:36:14 2007 5, 28 -- Received: from vic-MTAout4.csiro.au (vic-MTAout4.csiro.au [150.229.64.41]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7R2aDPU018118 5, 28 -- for {Microscopy-at-microscopy.com} ; Sun, 26 Aug 2007 21:36:13 -0500 5, 28 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=MvA+GXF8+pGN8rnUP0pkp0CuYiLpbLtNy59O+AZq86uIeKdxejCQBgg2or7PgGwFO2TrNeNrIHq89CtJ1K2FXhfi/t9DiYcWJkSb9jaUNq5wFeAMBH3eqQAgdbICViAG; 5, 28 -- X-IronPort-AV: E=Sophos;i="4.19,309,1183298400"; 5, 28 -- d="scan'208";a="146314697" 5, 28 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 5, 28 -- by vic-ironport-int.csiro.au with ESMTP; 27 Aug 2007 12:36:12 +1000 5, 28 -- Received: from EXSA4-ADL.nexus.csiro.au ([144.110.67.54]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 5, 28 -- Mon, 27 Aug 2007 12:36:12 +1000 5, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 5, 28 -- content-class: urn:content-classes:message 5, 28 -- MIME-Version: 1.0 5, 28 -- Content-Type: text/plain; 5, 28 -- charset="iso-8859-1" 5, 28 -- Subject: [Microscopy] RE: SEM: wt% or atom% ? 5, 28 -- Date: Mon, 27 Aug 2007 12:06:11 +0930 5, 28 -- Message-ID: {E811B633AE1E044493C956036D40C523746CD7-at-exsa4-adl.nexus.csiro.au} 5, 28 -- X-MS-Has-Attach: 5, 28 -- X-MS-TNEF-Correlator: 5, 28 -- Thread-Topic: [Microscopy] RE: SEM: wt% or atom% ? 5, 28 -- Thread-Index: AcfoUQp4ZsXR0SAHQFeHvrGr9bX/Xw== 5, 28 -- From: {Stuart.McClure-at-csiro.au} 5, 28 -- To: {Microscopy-at-microscopy.com} 5, 28 -- X-OriginalArrivalTime: 27 Aug 2007 02:36:12.0254 (UTC) FILETIME=[084CF7E0:01C7E853] 5, 28 -- Content-Transfer-Encoding: 8bit 5, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7R2aDPU018118 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wim.vandenbroeck-at-UGent.be as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wim.vandenbroeck-at-UGent.be Name: Wim Van den Broeck
Organization: Ghent University, Department of Morphology
Title-Subject: [Filtered] Leica EM TP programmes
Question: Dear Colleagues,
We have just installed the Leica EM tissue processor (TP), and I was wondering if programmes for the TP are available for processing animal tissues in Spurr's embedding medium. Thanks in advance.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pdcrystals-at-aol.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: pdcrystals-at-aol.com Name: Peter Droste
Organization: Surfacenet GmbH
Title-Subject: [Filtered] Surching for SEM spareparts
Question: Hallo to all,
I am surching for a power supply for a Philips 525 SEM stage system. It is labeled PE 1141/52 220V 5V +-15V
Is there any one who has such a part used or unused??
The Penn Regional Nanotechnology Facility is seeking to hire a research scientist with specialization in electron microscopy. The PRNTF is a central facilities laboratory for the University of Pennsylvania. We maintain a wide range of instrumentation, featuring a JEOL 2010F TEM/STEM, an FEI DB235 FIB, and a 5.1 MeV ion accelerator (see a complete facility description at www.seas.upenn.edu/nanotechfacility).
Principle duties of the research scientist will include assisting and training users, maintaining electron and ion beam instruments and developing new analytical techniques. Other duties include assisting with the teaching of laboratory classes, maintaining the user database and billing records, as well as maintaining the lab.
Requirements for this position include a BS in physical science or engineering (Masters or Ph.D. preferred), three to five years experience with the operation and maintenance of electron and ion beam instruments, and excellent communication skills. Preference will be given to individuals with experience using focused ion beam instruments and to those with experience in the development of analytical techniques.
Please note - This is not a postdoctoral position. Position is contingent upon funding.
For consideration, send resume/cv to:
Douglas Yates, Ph.D. Technical Director Penn Regional Nanotechnology Facility University of Pennsylvania 3231 Walnut Street Philadelphia, PA 19104 dmyates-at-seas.upenn.edu.
Additional information about the position may be viewed at: http://www.hr.upenn.edu/jobs/ (reference number: 070822872)
-- ******************************************************* Douglas M. Yates, Ph.D.
Technical Director Penn Regional Nanotechnology Facility University of Pennsylvania 3231 Walnut Street Philadelphia, PA 19104 http://www.seas.upenn.edu/nanotechfacility/
Include a check-out precedure, where you have to check the cleanliness of the place and undersign before they can leave.
Regards,
Stephane
--- gmartens-at-interchange.ubc.ca wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Debby, } } We have similar situation to your original } situation. Our main } processing microwave sits right next to the hood and } is vented } through a properly taped duct system. We try to } train our clients to } always use the vacuum chamber, regardless if they } are applying vacuum } or not. The chamber can be closed during the } transfer from the hood } to the microwave. } } Our main issue is getting people to clean up after } themselves. If } anyone has a nice way to convince your clients to } clean up after they } finish processing I would love to hear it. The } usual threats of } taking away privileges does not seem to work. } } Garnet } } -- } Garnet Martens } } Research Manager } BioImaging Facility } University of British Columbia } 6270 University Blvd. } Vancouver, B.C. } Canada } V6T 1Z4 } } phone 604-822-3354 } } ==============================Original } Headers============================== } 7, 29 -- From gmartens-at-interchange.ubc.ca Fri Aug 24 } 10:14:31 2007 } 7, 29 -- Received: from mr1.mail-relay.ubc.ca } (mr1.mail-relay.ubc.ca [137.82.45.1]) } 7, 29 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } l7OFEV1U003262 } 7, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, } 24 Aug 2007 10:14:31 -0500 } 7, 29 -- X-Ubc-Received: from mr1.mail-relay.ubc.ca } (localhost [127.0.0.1]) } 7, 29 -- by localhost (Postfix) with SMTP id } BB276ECA9 } 7, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, } 24 Aug 2007 08:14:30 -0700 (PDT) } 7, 29 -- Received: from mta2.interchange.ubc.ca } (mta2.interchange.ubc.ca [142.103.145.70]) } 7, 29 -- by mr1.mail-relay.ubc.ca (Postfix) with } ESMTP } 7, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, } 24 Aug 2007 08:14:30 -0700 (PDT) } 7, 29 -- Received: from [137.82.85.216] } (0511.emlab.ubc.ca [137.82.85.216]) } 7, 29 -- by smtp.interchange.ubc.ca } 7, 29 -- (iPlanet Messaging Server 5.2 HotFix 1.21 } (built Sep 8 2003)) } 7, 29 -- with ESMTPA id } {0JNA003UVAC5GU-at-smtp.interchange.ubc.ca} for } 7, 29 -- Microscopy-at-MSA.Microscopy.Com; Fri, 24 Aug } 2007 08:14:29 -0700 (PDT) } 7, 29 -- Date: Fri, 24 Aug 2007 08:15:14 -0700 } 7, 29 -- From: Garnet Martens } {gmartens-at-interchange.ubc.ca} } 7, 29 -- Subject: Re: [Microscopy] Safety issues } with microwave } 7, 29 -- In-reply-to: } {200708241415.l7OEFouf008498-at-ns.microscopy.com} } 7, 29 -- To: Microscopy Listserver } {Microscopy-at-MSA.Microscopy.Com} } 7, 29 -- Message-id: } {a06240805c2f4a52991fc-at-[137.82.85.216]} } 7, 29 -- MIME-version: 1.0 } 7, 29 -- Content-type: text/plain; format=flowed; } charset=us-ascii } 7, 29 -- References: } {200708241415.l7OEFouf008498-at-ns.microscopy.com} } 7, 29 -- X-UBC-Scanned: Sophos PureMessage } 5.3.3.310218, Antispam-Engine: 2.5.2.311128, } Antispam-Data: 2007.8.24.74423 } 7, 29 -- X-UBC-Relayed: Relayed through } mail-relay.ubc.ca } 7, 29 -- X-PerlMx-Spam: Probability=7%, } Report=BODY_SIZE_800_899 0, __C230066_P5 0, __CT 0, } __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY } 0, __MIME_VERSION 0, __SANE_MSGID 0 } 7, 29 -- X-Spam-Level: } 7, 29 -- X-Spam-Flag: No } ==============================End of - } Headers============================== }
____________________________________________________________________________________ Pinpoint customers who are looking for what you sell. http://searchmarketing.yahoo.com/
____________________________________________________________________________________ Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. http://mobile.yahoo.com/go?refer=1GNXIC
==============================Original Headers============================== 11, 20 -- From nizets2-at-yahoo.com Mon Aug 27 10:38:12 2007 11, 20 -- Received: from web37410.mail.mud.yahoo.com (web37410.mail.mud.yahoo.com [209.191.91.142]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l7RFcBIK016641 11, 20 -- for {microscopy-at-microscopy.com} ; Mon, 27 Aug 2007 10:38:11 -0500 11, 20 -- Received: (qmail 18826 invoked by uid 60001); 27 Aug 2007 15:38:11 -0000 11, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 20 -- s=s1024; d=yahoo.com; 11, 20 -- h=Received:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 11, 20 -- b=sJ9vbhfiDATfkdgjYZrQC4hyIQ48XWAEndab6SWRU//xzWDDpgl7i3aLE7RgzyIW8Mr4K+M63L43Izp2PYMawNRPyBoMxNIe7QEd3h/p+PKYXcu+rHtG1jDlZaQaoRrn5g3w12I+Nzt/qIdvTuiOU/U7iDaFLkRPVt7sI684tr8=; 11, 20 -- Received: from [80.122.101.102] by web37410.mail.mud.yahoo.com via HTTP; Mon, 27 Aug 2007 08:38:11 PDT 11, 20 -- Date: Mon, 27 Aug 2007 08:38:11 -0700 (PDT) 11, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 11, 20 -- Subject: Re: [Microscopy] Re: Safety issues with microwave 11, 20 -- To: gmartens-at-interchange.ubc.ca 11, 20 -- Cc: microscopy-at-microscopy.com 11, 20 -- In-Reply-To: {200708241520.l7OFKxYi018063-at-ns.microscopy.com} 11, 20 -- MIME-Version: 1.0 11, 20 -- Content-Type: text/plain; charset=iso-8859-1 11, 20 -- Content-Transfer-Encoding: 8bit 11, 20 -- Message-ID: {465765.18679.qm-at-web37410.mail.mud.yahoo.com} ==============================End of - Headers==============================
I am interesting in microscope history, and collect some information about microscope history. I am going to create CD-ROM about microscope history. Is it interesting for anybody in Microscopiy Society of America?
Best regards Nikolay Koltovoy
} } my e-mail koltovoi-at-mail.ru
-- Greg Erdos University of Florida, Retired Micanopy, Florida
==============================Original Headers============================== 11, 23 -- From gwe-at-ufl.edu Mon Aug 27 13:00:36 2007 11, 23 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7RI0abi032399 11, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 27 Aug 2007 13:00:36 -0500 11, 23 -- Received: from [10.228.0.118] (ssrb-vpn1-0-118.vpn.ufl.edu [10.228.0.118]) 11, 23 -- (authenticated bits=0) 11, 23 -- by smtp.ufl.edu (8.13.7/8.13.7/2.5.9) with ESMTP id l7RI0X8Z1372310 11, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 27 Aug 2007 14:00:34 -0400 11, 23 -- Message-ID: {46D3113D.90205-at-ufl.edu} 11, 23 -- Date: Mon, 27 Aug 2007 14:00:29 -0400 11, 23 -- From: Greg Erdos {gwe-at-ufl.edu} 11, 23 -- Reply-To: gwe-at-ufl.edu 11, 23 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 11, 23 -- MIME-Version: 1.0 11, 23 -- To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com} 11, 23 -- Subject: [Fwd: Re Russia] 11, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 23 -- Content-Transfer-Encoding: 7bit 11, 23 -- X-Greylist: Sender succeeded SMTP AUTH authentication, not delayed by milter-greylist-3.0rc3 (smtp.ufl.edu [128.227.74.56]); Mon, 27 Aug 2007 14:00:34 -0400 (EDT) 11, 23 -- X-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 11, 23 -- X-UFL-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 11, 23 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 11, 23 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
We have a group here studying calcified bone. They came into our histology core with sections from methacrylate-embedded bone and asked if we could perform special stains on the sections. The EM lab here doesn't work with methacrylates, so we got the job.
A LONG time ago I did a little work with these plastics, and I recall that they are better than epoxy plastics for a variety of stains. I also recall that the protocols had to be modified from the standard paraffin/frozen section recipes to compensate for the methacrylate.
They would like to do: Safronin O Fast Green Methylene Blue Picosirus Red
My histotech is working her connections and I'm hoping that there's someone on this list that can give me some pointers.
Thanks Doug
-- ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Douglas W. Cromey, M.S. - Assistant Scientific Investigator Dept. of Cell Biology & Anatomy, University of Arizona 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
Burns WA, Bretschneider A (1981) Thin is In: Plastic Embedding of Tissue for Light Microscopy. Chicago, American Society of Clinical Pathologists
I am pretty sure that I remember it has a lot of stains for methacrylates
At 01:12 PM 08/27/07, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
Someone wants to know if I can use TEM to look for herpes virus in paraffin embedded tissue. It was fixed in formalin. I told them it would look like doo-doo (scientific term), but if they insisted, we could try. If I rememer correctly, the way to go about this is to de-paraffinize with xylene, then go into ethanol and/or propylene oxide, and embed in epoxy resin (I use LX112). Am I on the right track? Any help appreciated.
Any for any of you virologists or anyone else who has tried this, what are the chances of being able to recognize herpes in tumor nuclei after this abuse? Will this be a big waste of time? No, can't re-do experiment.
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 6, 19 -- From tina-at-pbrc.hawaii.edu Mon Aug 27 14:26:37 2007 6, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7RJQYXo016337 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 27 Aug 2007 14:26:34 -0500 6, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id l7RJQTfx005478 6, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 27 Aug 2007 09:26:30 -1000 (HST) 6, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id l7RJQSmI005475 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 27 Aug 2007 09:26:29 -1000 (HST) 6, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 6, 19 -- Date: Mon, 27 Aug 2007 09:26:28 -1000 (HST) 6, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 19 -- X-Sender: tina-at-halia 6, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 6, 19 -- Subject: Deparaffinizing tissue for TEM embedding 6, 19 -- Message-ID: {Pine.GSO.4.21.0708270920480.5367-100000-at-halia} 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
It is probably a 30L/s Varian pump. Duniway Stock Room rebuilds and sells these for about $500US. Pull the magnet off and repair or exchange the core. Be sure to get a new Copper gasket as well.
gary g.
At 11:10 AM 8/27/2007, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Mon Aug 27 14:57:40 2007 10, 20 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l7RJver5028844 10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 27 Aug 2007 14:57:40 -0500 10, 20 -- Message-Id: {200708271957.l7RJver5028844-at-ns.microscopy.com} 10, 20 -- Received: (qmail 9968 invoked from network); 27 Aug 2007 12:57:40 -0700 10, 20 -- Received: by simscan 1.1.0 ppid: 9961, pid: 9965, t: 0.1437s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp2 with SMTP; 27 Aug 2007 12:57:40 -0700 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Mon, 27 Aug 2007 12:57:42 -0800 10, 20 -- To: filipi-at-pucrs.br 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] Ion pump needed 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200708271910.l7RJAhJh006950-at-ns.microscopy.com} 10, 20 -- References: {200708271910.l7RJAhJh006950-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-2CCD6C4E ==============================End of - Headers==============================
Dear colleagues I am looking for one [potentially two] postdoctoral fellows interested in advanced applications of Piezoresponse Force Microscopy to nanoelectromechanics of nanoscale ferroelectrics and multiferroics, macromolecular systems, and biological systems. Please see the announcement at: http://www.orau.gov/ORISE/edu/ornl/postneeds.htm [position ORNL07-70-CNMS]. Sergei Kalinin
Project Description:
The Center for Nanophase Materials Science (CNMS) at Oak Ridge National Laboratory (ORNL) is seeking a candidate to fill a postdoctoral position in the field of scanning probe microscopy. This program takes advantage of recently developed capabilities for Piezoresponse Force Microscopy (PFM) in liquid and ultra-high vacuum environments, and new spectroscopic modes for studies of polarization dynamics and correlated phenomena in a wide range of materials, including oxides, polymers, and biological systems. The CNMS (http://cnms.ornl.gov) is a collaborative nanoscience user research facility established by the Office of Science, U.S. Department of Energy. The CNMS has a diverse spectrum of nanoscience research activities including a nanofabrication facility; laboratory-based research on macromolecular materials, catalysts, functional nanomaterials, and magnetism and transport; characterization with electron microscopes, scanning probes, and x-ray diffraction and scattering; and theory, modeling, and simulation.
The successful applicant must demonstrate experience in the experimental application, development, and quantitative interpretation of modern scanning probe techniques. This position provides an opportunity to join an experienced team to develop methods and applications in directions such as identification and control of static and dynamic ferroelectric phenomena at the nanometer scale, electromechanical probing on the single-molecule level, and time-resolved measurements. In addition, the candidate will be involved in the CNMS user program, with wide opportunities for scientific collaborations.
==============================Original Headers============================== 4, 24 -- From sergei2-at-ornl.gov Mon Aug 27 17:19:05 2007 4, 24 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7RMJ5ME011082 4, 24 -- for {microscopy-at-microscopy.com} ; Mon, 27 Aug 2007 17:19:05 -0500 4, 24 -- Received: from emroute1.ornl.gov ([127.0.0.1]) 4, 24 -- by emroute1.ornl.gov (PMDF V6.3-x11 #31501) 4, 24 -- with ESMTP id {0JNG006C1DZSKF-at-emroute1.ornl.gov} for 4, 24 -- microscopy-at-microscopy.com; Mon, 27 Aug 2007 18:19:04 -0400 (EDT) 4, 24 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov 4, 24 -- (PMDF V6.3-x11 #31501) id {0JNG00901DZS7O-at-emroute1.ornl.gov} ; Mon, 4, 24 -- 27 Aug 2007 18:19:04 -0400 (EDT) 4, 24 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 4, 24 -- by emroute1.ornl.gov (PMDF V6.3-x11 #31501) 4, 24 -- with ESMTP id {0JNG0058UDZRWA-at-emroute1.ornl.gov} ; Mon, 4, 24 -- 27 Aug 2007 18:19:03 -0400 (EDT) 4, 24 -- Date: Mon, 27 Aug 2007 18:19:03 -0400 4, 24 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 4, 24 -- Subject: ORNL Postdoctoral position - Nanoscale Electromechanics 4, 24 -- To: spm-at-spmlist.di.com, microscopy-at-microscopy.com 4, 24 -- Message-id: {46D34DD7.9080902-at-ornl.gov} 4, 24 -- MIME-version: 1.0 4, 24 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 4, 24 -- Content-transfer-encoding: 7bit 4, 24 -- User-Agent: Thunderbird 1.5.0.13 (Windows/20070809) ==============================End of - Headers==============================
Hi Tina, I am frequently asked to do the same task. I discourage the pathologists because the preservation of the tissue is almost none. However you can detect viral particles and if it is done solely for that purpose it can work. Since there is no membrane preservation nucleus can only be distinguished by the presence of a chromatin. I prefer to process the tissue that has been stored in formalin even for a long time. Good luck. Dorota.
==============================Original Headers============================== 1, 24 -- From wadowska-at-avcn1.novell.upei.ca Tue Aug 28 06:20:25 2007 1, 24 -- Received: from mx2.upei.ca (magellanic.cs.upei.ca [137.149.3.22]) 1, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7SBKPUw009762 1, 24 -- for {microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 06:20:25 -0500 1, 24 -- Received: from [137.149.64.250] (helo=acad1.cs.upei.ca) 1, 24 -- by mx2.upei.ca with esmtp (Exim 4.50 #1 (Debian)) 1, 24 -- id 1IPz6r-0008NV-BE 1, 24 -- for {microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 08:19:49 -0300 1, 24 -- Received: from AVCN1/SpoolDir by acad1.cs.upei.ca (Mercury 1.48); 1, 24 -- 28 Aug 07 08:20:24 -0300 1, 24 -- Received: from SpoolDir by AVCN1 (Mercury 1.48); 28 Aug 07 08:20:08 -0300 1, 24 -- From: "Dorota Wadowska" {wadowska-at-upei.ca} 1, 24 -- Organization: University of P.E.I. 1, 24 -- To: microscopy-at-microscopy.com 1, 24 -- Date: Tue, 28 Aug 2007 08:17:45 -0400 1, 24 -- MIME-Version: 1.0 1, 24 -- Content-type: text/plain; charset=US-ASCII 1, 24 -- Content-transfer-encoding: 7BIT 1, 24 -- Subject: Deparaffinizing tissue for TEM embedding 1, 24 -- Message-ID: {46D3DA28.13316.F7882-at-localhost} 1, 24 -- X-Confirm-Reading-To: "Dorota Wadowska" {wadowska-at-acad1.cs.upei.ca} 1, 24 -- X-pmrqc: 1 1, 24 -- Priority: normal 1, 24 -- X-mailer: Pegasus Mail for Win32 (v3.12c) ==============================End of - Headers==============================
I am having trouble getting my power supply to strike my HBO 100. I checked the bulb on another microscope and the bulb is good. The power supply will come on but never strikes the bulb. I asked our electrician to help and he asked me what initial arc voltage is needed to start the bulb. I did not have an answer and can not seem to find the answer. I was hoping that someone on this list could help me.
Thanks in advance, Lori Ables laable-at-solutia.com
This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain Solutia confidential and privileged information. The recipient is hereby put on notice to treat the information as confidential and privileged and to not disclose or use the information except as authorized by Solutia. Any unauthorized review, printing, retention, copying, disclosure, distribution, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you received this message in error, please immediately contact the sender by reply email and delete all copies of the material from any computer. Thank you for your cooperation.
==============================Original Headers============================== 6, 33 -- From laable-at-solutia.com Tue Aug 28 10:17:25 2007 6, 33 -- Received: from ahmler4.mail.eds.com (ahmler4.mail.eds.com [192.85.154.77]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7SFHPe5028158 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Tue, 28 Aug 2007 10:17:25 -0500 6, 33 -- Received: from ahmlir1.mail.eds.com (ahmlir1-2.mail.eds.com [192.85.154.131]) 6, 33 -- by ahmler4.mail.eds.com (8.13.8/8.13.8) with ESMTP id l7SFHNn2020809 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Tue, 28 Aug 2007 11:17:25 -0400 6, 33 -- Received: from ahmlir1.mail.eds.com (localhost [127.0.0.1]) 6, 33 -- by ahmlir1.mail.eds.com (8.13.8/8.12.10) with ESMTP id l7SFHJW8025194 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Tue, 28 Aug 2007 11:17:19 -0400 6, 33 -- Received: from mailgw2.solutia.com ([205.191.166.96]) 6, 33 -- by ahmlir1.mail.eds.com (8.13.8/8.12.10) with ESMTP id l7SFHJQ9025186 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Tue, 28 Aug 2007 11:17:19 -0400 6, 33 -- Received: from USAHMSLSOIEX2.soi.dir.solutia.com ([205.191.166.99]) by mailgw2.solutia.com with Microsoft SMTPSVC(6.0.3790.1830); Tue, 28 Aug 2007 11:17:18 -0400 6, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.2929 6, 33 -- Content-Class: urn:content-classes:message 6, 33 -- MIME-Version: 1.0 6, 33 -- Content-Type: text/plain; 6, 33 -- charset="us-ascii" 6, 33 -- Subject: Fluorescence Microscopy 6, 33 -- Date: Tue, 28 Aug 2007 11:17:17 -0400 6, 33 -- Message-ID: {7B97BC1F2986504EABA8A0B1C780A5C302263F3A-at-USAHMSLSOIEX2.soi.dir.solutia.com} 6, 33 -- Importance: normal 6, 33 -- Priority: normal 6, 33 -- X-MS-Has-Attach: 6, 33 -- X-MS-TNEF-Correlator: 6, 33 -- Thread-Topic: Fluorescence Microscopy 6, 33 -- thread-index: AcfphoUDJM7ymJd3T9K1vfxelAtnew== 6, 33 -- From: "Ables, Lori A" {laable-at-solutia.com} 6, 33 -- To: {Microscopy-at-Microscopy.Com} 6, 33 -- X-OriginalArrivalTime: 28 Aug 2007 15:17:18.0605 (UTC) FILETIME=[85FAE3D0:01C7E986] 6, 33 -- Content-Transfer-Encoding: 8bit 6, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7SFHPe5028158 ==============================End of - Headers==============================
I don't know the arc voltage but we have had a frequent similar problem. It actually ended up being the saftey mechanism in the lamp housing that prevents the lamp from igniting when opened. The door must close and complete a curcuit. This contact gets dirty and then prevents the lamp from igniting. This is a easy fix if it is the scource of your problem and it doesn't require knowing any numbers. Hope this solves it.
Robert Underwood University of Washington
On Tue, 28 Aug 2007 laable-at-solutia.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } I am having trouble getting my power supply to strike my HBO 100. I checked the bulb on another microscope and the bulb is good. The power supply will come on but never strikes the bulb. I asked our electrician to help and he asked me what initial arc voltage is needed to start the bulb. I did not have an answer and can not seem to find the answer. I was hoping that someone on this list could help me. } } Thanks in advance, } Lori Ables } laable-at-solutia.com } } } This electronic mail message is intended exclusively for the individual or entity to which it is addressed. } This message, together with any attachment, may contain Solutia confidential and privileged information. } The recipient is hereby put on notice to treat the information as confidential and privileged and to not disclose or use the information except as authorized by Solutia. } Any unauthorized review, printing, retention, copying, disclosure, distribution, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you received this message in error, please immediately contact the sender by reply email and delete all copies of the material from any computer. Thank you for your cooperation. } } } ==============================Original Headers============================== } 6, 33 -- From laable-at-solutia.com Tue Aug 28 10:17:25 2007 } 6, 33 -- Received: from ahmler4.mail.eds.com (ahmler4.mail.eds.com [192.85.154.77]) } 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7SFHPe5028158 } 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Tue, 28 Aug 2007 10:17:25 -0500 } 6, 33 -- Received: from ahmlir1.mail.eds.com (ahmlir1-2.mail.eds.com [192.85.154.131]) } 6, 33 -- by ahmler4.mail.eds.com (8.13.8/8.13.8) with ESMTP id l7SFHNn2020809 } 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Tue, 28 Aug 2007 11:17:25 -0400 } 6, 33 -- Received: from ahmlir1.mail.eds.com (localhost [127.0.0.1]) } 6, 33 -- by ahmlir1.mail.eds.com (8.13.8/8.12.10) with ESMTP id l7SFHJW8025194 } 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Tue, 28 Aug 2007 11:17:19 -0400 } 6, 33 -- Received: from mailgw2.solutia.com ([205.191.166.96]) } 6, 33 -- by ahmlir1.mail.eds.com (8.13.8/8.12.10) with ESMTP id l7SFHJQ9025186 } 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Tue, 28 Aug 2007 11:17:19 -0400 } 6, 33 -- Received: from USAHMSLSOIEX2.soi.dir.solutia.com ([205.191.166.99]) by mailgw2.solutia.com with Microsoft SMTPSVC(6.0.3790.1830); Tue, 28 Aug 2007 11:17:18 -0400 } 6, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.2929 } 6, 33 -- Content-Class: urn:content-classes:message } 6, 33 -- MIME-Version: 1.0 } 6, 33 -- Content-Type: text/plain; } 6, 33 -- charset="us-ascii" } 6, 33 -- Subject: Fluorescence Microscopy } 6, 33 -- Date: Tue, 28 Aug 2007 11:17:17 -0400 } 6, 33 -- Message-ID: {7B97BC1F2986504EABA8A0B1C780A5C302263F3A-at-USAHMSLSOIEX2.soi.dir.solutia.com} } 6, 33 -- Importance: normal } 6, 33 -- Priority: normal } 6, 33 -- X-MS-Has-Attach: } 6, 33 -- X-MS-TNEF-Correlator: } 6, 33 -- Thread-Topic: Fluorescence Microscopy } 6, 33 -- thread-index: AcfphoUDJM7ymJd3T9K1vfxelAtnew== } 6, 33 -- From: "Ables, Lori A" {laable-at-solutia.com} } 6, 33 -- To: {Microscopy-at-Microscopy.Com} } 6, 33 -- X-OriginalArrivalTime: 28 Aug 2007 15:17:18.0605 (UTC) FILETIME=[85FAE3D0:01C7E986] } 6, 33 -- Content-Transfer-Encoding: 8bit } 6, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7SFHPe5028158 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 22 -- From underwoo-at-u.washington.edu Tue Aug 28 11:03:10 2007 9, 22 -- Received: from mxout7.cac.washington.edu (mxout7.cac.washington.edu [140.142.32.178]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7SG3AN5008712 9, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 28 Aug 2007 11:03:10 -0500 9, 22 -- Received: from hymn10.u.washington.edu (hymn10.u.washington.edu [140.142.13.244]) 9, 22 -- by mxout7.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.06) with ESMTP id l7SG3929006139 9, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 9, 22 -- Tue, 28 Aug 2007 09:03:09 -0700 9, 22 -- Received: from localhost (localhost [127.0.0.1]) 9, 22 -- by hymn10.u.washington.edu (8.13.7+UW06.06/8.13.7+UW07.03) with ESMTP id l7SG39HX024047; 9, 22 -- Tue, 28 Aug 2007 09:03:09 -0700 9, 22 -- X-Auth-Received: from [128.208.106.224] by hymn10.u.washington.edu via HTTP; Tue, 28 Aug 2007 09:03:09 PDT 9, 22 -- Date: Tue, 28 Aug 2007 09:03:09 -0700 (PDT) 9, 22 -- From: Robert A Underwood {underwoo-at-u.washington.edu} 9, 22 -- To: laable-at-solutia.com, Microscopy List {Microscopy-at-MSA.Microscopy.Com} 9, 22 -- Subject: [Microscopy] Re:Fluorescence Microscopy 9, 22 -- In-Reply-To: {200708281520.l7SFKRPe030246-at-ns.microscopy.com} 9, 22 -- Message-ID: {Pine.LNX.4.43.0708280903090.6038-at-hymn10.u.washington.edu} 9, 22 -- MIME-Version: 1.0 9, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 9, 22 -- X-PMX-Version: 5.3.3.310218, Antispam-Engine: 2.5.1.298604, Antispam-Data: 2007.8.28.83725 9, 22 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0' ==============================End of - Headers==============================
Could someone share some light and a protocol to achieve H&E staining on epoxy-embedded tissue sections (heart muscle)? I simply cannot get the hematoxylin to stain nuclei and yields a general "grayish" stain of components. No heat yields absolutely no staining. Alcoholic eosin Y has no problem yielding a bright pink. Maybe too well. Or this simply why I never hear of H&E staining in EM? Thanks!
Walter F. Bobrowski Sr. Scientist Pfizer Global R&D
"The ultimate human freedom is the ability to choose one's attitude in a given set of circumstances." -Viktor Frankl
---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately.
==============================Original Headers============================== 6, 30 -- From Walter.Bobrowski-at-pfizer.com Tue Aug 28 11:37:18 2007 6, 30 -- Received: from mopmsgoa01.pfizer.com (mopmsgo.pfizer.com [148.168.100.84]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7SGbI0T021156 6, 30 -- for {microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 11:37:18 -0500 6, 30 -- Received: from mopamrexc02.amer.pfizer.com (mopamrexc02.pfizer.com [170.116.30.68]) 6, 30 -- by mopmsgoa01.pfizer.com (8.13.7/8.13.7) with ESMTP id l7SGbHQw001311 6, 30 -- for {microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 12:37:17 -0400 6, 30 -- Received: from mopamrexc03.amer.pfizer.com ([170.116.30.69]) by mopamrexc02.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.1830); 6, 30 -- Tue, 28 Aug 2007 12:37:17 -0400 6, 30 -- Received: from anaamrexm01.amer.pfizer.com ([162.48.101.10]) by mopamrexc03.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.1830); 6, 30 -- Tue, 28 Aug 2007 12:37:17 -0400 6, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 30 -- Content-class: urn:content-classes:message 6, 30 -- MIME-Version: 1.0 6, 30 -- Content-Type: text/plain; 6, 30 -- charset="us-ascii" 6, 30 -- Subject: H&E Staining of Epoxy-embedded Tissues 6, 30 -- Date: Tue, 28 Aug 2007 12:37:20 -0400 6, 30 -- Message-ID: {CF89AF6ABD93B746A55BCCD9480A4B3D09987C85-at-anaamrexm01.amer.pfizer.com} 6, 30 -- X-MS-Has-Attach: 6, 30 -- X-MS-TNEF-Correlator: 6, 30 -- Thread-Topic: H&E Staining of Epoxy-embedded Tissues 6, 30 -- Thread-Index: AcfpkbRJyrgIgnoxTHelNq81hogE/g== 6, 30 -- From: "Bobrowski, Walter" {Walter.Bobrowski-at-pfizer.com} 6, 30 -- To: {microscopy-at-microscopy.com} 6, 30 -- X-OriginalArrivalTime: 28 Aug 2007 16:37:17.0380 (UTC) FILETIME=[B245D840:01C7E991] 6, 30 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=4.65.5502:2.3.11,1.2.37,4.0.164 definitions=2007-08-28_07:2007-08-28,2007-08-28,2007-08-28 signatures=0 6, 30 -- X-Proofpoint-Spam-Reason: safe 6, 30 -- Content-Transfer-Encoding: 8bit 6, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7SGbI0T021156 ==============================End of - Headers==============================
I have quite a bit of experience with an inexpensive program (Helicon Focus) and a free program (CombineZ) and both can do an excellent job in many instances. I have tried an ImageJ plug-in (Extended_Depth_Field.jar) with less success. I know that software for combining the in-focus regions of Z-stacks are available from microscope manufacturers and as part of image analysis packages. Has any member of the group compared other programs to Helicon Focus or CombineZ? Do any of them offer any significant improvements in handling "problem stacks"? The main issues for me are areas where features overlap in different planes of focus, ghost fringes around edges, and specular highlights that spread as frames are combined.
Ralph Common Michigan State University
==============================Original Headers============================== 4, 24 -- From rcommon-at-msu.edu Tue Aug 28 11:48:00 2007 4, 24 -- Received: from sys27.mail.msu.edu (sys27.mail.msu.edu [35.9.75.127]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7SGm0Xq000548 4, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 11:48:00 -0500 4, 24 -- Received: from [35.9.122.125] (helo=emlab) 4, 24 -- by sys27.mail.msu.edu with esmtpsa (Exim 4.63 #1) 4, 24 -- (TLSv1:RC4-MD5:128) 4, 24 -- id 1IQ4ES-0007D0-J5 4, 24 -- for Microscopy-at-microscopy.com; Tue, 28 Aug 2007 12:48:00 -0400 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: Extended depth of field photomacrography 4, 24 -- Date: Tue, 28 Aug 2007 12:49:21 -0400 4, 24 -- Message-ID: {002f01c7e993$63770fc0$7d7a0923-at-msu.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="iso-8859-1" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 (Normal) 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 4, 24 -- Importance: Normal 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1896 4, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
We used to use .1-1% Toluidine Blue in Sodium Borate to stain epoxy sections. It is not the same as Hematoxylin, but will certainly give you a pretty blue in the nuclei.
Joel
Date sent: Tue, 28 Aug 2007 11:37:49 -0500 To: jbs-at-temple.edu X-from: Walter.Bobrowski-at-pfizer.com Send reply to: Walter.Bobrowski-at-pfizer.com
I would just add some details. I have used 0.05% Toluidine Blue O in 1% sodium borate buffer. Dissolve with stirbar and filter through Whatman #1 or equivalent paper. After flattening and drying down the sections in a small drop of water at ~ 60C (can be over an alcohol lamp or heat plate, don't boil), flood with a large drop of the stain and continue heating - view white paper through the drop to see density; or time...; rinse with dH2O from a squirt bottle gently to remove the excess stain, and dry. Mount in a small drop of immersion oil with a coverglass for 40x and greater; seal edges with nail polish if needed. Keep slides from strong light - it fades eventually but can last months to years. Toluidine Blue is metachromatic and will show materials with chemistry dependent characteristic colors - for plants, acidic polysaccharies as pink-ish, lignified walls of plant vasculature is often ice-blue, etc.
dale callaham
jbs-at-temple.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We used to use .1-1% Toluidine Blue in Sodium Borate to stain epoxy } sections. It is not the same as Hematoxylin, but will certainly give } you a pretty blue in the nuclei. } } Joel } } Date sent: Tue, 28 Aug 2007 11:37:49 -0500 } To: jbs-at-temple.edu } X-from: Walter.Bobrowski-at-pfizer.com } Send reply to: Walter.Bobrowski-at-pfizer.com } Subject: [Microscopy] H&E Staining of Epoxy-embedded Tissues } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Could someone share some light and a protocol to achieve H&E staining on } } epoxy-embedded tissue sections (heart muscle)? I simply cannot get the } } hematoxylin to stain nuclei and yields a general "grayish" stain of } } components. No heat yields absolutely no staining. Alcoholic eosin Y has } } no problem yielding a bright pink. Maybe too well. Or this simply why I } } never hear of H&E staining in EM? } } Thanks! } } } } } } Walter F. Bobrowski } } Sr. Scientist } } Pfizer Global R&D } } } } } } "The ultimate human freedom is the ability to choose one's attitude in a } } given set of circumstances." -Viktor Frankl } } } } ---------------------------------------------------------------------- } } LEGAL NOTICE } } Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. } } } } } } ==============================Original Headers============================== } } 6, 30 -- From Walter.Bobrowski-at-pfizer.com Tue Aug 28 11:37:18 2007 } } 6, 30 -- Received: from mopmsgoa01.pfizer.com (mopmsgo.pfizer.com [148.168.100.84]) } } 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7SGbI0T021156 } } 6, 30 -- for {microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 11:37:18 -0500 } } 6, 30 -- Received: from mopamrexc02.amer.pfizer.com (mopamrexc02.pfizer.com [170.116.30.68]) } } 6, 30 -- by mopmsgoa01.pfizer.com (8.13.7/8.13.7) with ESMTP id l7SGbHQw001311 } } 6, 30 -- for {microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 12:37:17 -0400 } } 6, 30 -- Received: from mopamrexc03.amer.pfizer.com ([170.116.30.69]) by mopamrexc02.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.1830); } } 6, 30 -- Tue, 28 Aug 2007 12:37:17 -0400 } } 6, 30 -- Received: from anaamrexm01.amer.pfizer.com ([162.48.101.10]) by mopamrexc03.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.1830); } } 6, 30 -- Tue, 28 Aug 2007 12:37:17 -0400 } } 6, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } } 6, 30 -- Content-class: urn:content-classes:message } } 6, 30 -- MIME-Version: 1.0 } } 6, 30 -- Content-Type: text/plain; } } 6, 30 -- charset="us-ascii" } } 6, 30 -- Subject: H&E Staining of Epoxy-embedded Tissues } } 6, 30 -- Date: Tue, 28 Aug 2007 12:37:20 -0400 } } 6, 30 -- Message-ID: {CF89AF6ABD93B746A55BCCD9480A4B3D09987C85-at-anaamrexm01.amer.pfizer.com} } } 6, 30 -- X-MS-Has-Attach: } } 6, 30 -- X-MS-TNEF-Correlator: } } 6, 30 -- Thread-Topic: H&E Staining of Epoxy-embedded Tissues } } 6, 30 -- Thread-Index: AcfpkbRJyrgIgnoxTHelNq81hogE/g== } } 6, 30 -- From: "Bobrowski, Walter" {Walter.Bobrowski-at-pfizer.com} } } 6, 30 -- To: {microscopy-at-microscopy.com} } } 6, 30 -- X-OriginalArrivalTime: 28 Aug 2007 16:37:17.0380 (UTC) FILETIME=[B245D840:01C7E991] } } 6, 30 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=4.65.5502:2.3.11,1.2.37,4.0.164 definitions=2007-08-28_07:2007-08-28,2007-08-28,2007-08-28 signatures=0 } } 6, 30 -- X-Proofpoint-Spam-Reason: safe } } 6, 30 -- Content-Transfer-Encoding: 8bit } } 6, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7SGbI0T021156 } } ==============================End of - Headers============================== } } } -- } Joel B. Sheffield, Ph.D. } Biology Department, Temple University } 1900 North 12th Street } Philadelphia, PA 19122 } jbs-at-temple.edu } (215) 204 8839, fax (215) 204 0486 } http://astro.temple.edu/~jbs } } } ==============================Original Headers============================== } 7, 21 -- From jbs-at-temple.edu Tue Aug 28 12:28:01 2007 } 7, 21 -- Received: from imp1.temple.edu (imp1.ocis.temple.edu [155.247.166.81]) } 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7SHS0hs013252 } 7, 21 -- for {microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 12:28:01 -0500 } 7, 21 -- Received: from [155.247.98.40] (jbs.bio.temple.edu [155.247.98.40]) } 7, 21 -- by imp1.temple.edu (8.12.3/8.11.3/SuSE Linux 8.11.1-0.5) with ESMTP id l7SHS0wk007801; } 7, 21 -- Tue, 28 Aug 2007 13:28:00 -0400 } 7, 21 -- From: "Joel Sheffield" {jbs-at-temple.edu} } 7, 21 -- To: Walter.Bobrowski-at-pfizer.com, microscopy-at-microscopy.com } 7, 21 -- Date: Tue, 28 Aug 2007 13:28:06 -0400 } 7, 21 -- MIME-Version: 1.0 } 7, 21 -- Subject: Re: [Microscopy] H&E Staining of Epoxy-embedded Tissues } 7, 21 -- Reply-to: jbs-at-temple.edu } 7, 21 -- Message-ID: {46D422E6.22201.1FB950C3-at-jbs.temple.edu} } 7, 21 -- Priority: normal } 7, 21 -- In-reply-to: {200708281637.l7SGbn2R021326-at-ns.microscopy.com} } 7, 21 -- References: {200708281637.l7SGbn2R021326-at-ns.microscopy.com} } 7, 21 -- X-mailer: Pegasus Mail for Windows (4.41) } 7, 21 -- Content-type: text/plain; charset=US-ASCII } 7, 21 -- Content-transfer-encoding: 7BIT } 7, 21 -- Content-description: Mail message body } ==============================End of - Headers==============================
==============================Original Headers============================== 3, 22 -- From dac-at-research.umass.edu Tue Aug 28 12:48:30 2007 3, 22 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37]) 3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7SHmUMR025279 3, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 12:48:30 -0500 3, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 3, 22 -- (authenticated bits=0) 3, 22 -- by race1.oit.umass.edu (8.13.7/8.13.7) with ESMTP id l7SHmUpC001396 3, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 3, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 13:48:30 -0400 3, 22 -- Message-ID: {46D46F04.8040401-at-research.umass.edu} 3, 22 -- Date: Tue, 28 Aug 2007 13:52:52 -0500 3, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 3, 22 -- Reply-To: "dac-at-research.umass.edu } } Dale Callaham" {dac-at-research.umass.edu} 3, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.6) Gecko/20070802 SeaMonkey/1.1.4 3, 22 -- MIME-Version: 1.0 3, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 3, 22 -- Subject: Re: [Microscopy] Re: H&E Staining of Epoxy-embedded Tissues 3, 22 -- References: {200708281735.l7SHZ9t5022568-at-ns.microscopy.com} 3, 22 -- In-Reply-To: {200708281735.l7SHZ9t5022568-at-ns.microscopy.com} 3, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 22 -- Content-Transfer-Encoding: 7bit 3, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
My thanks for everyone's invaluable input. With the responses provided, I was able to make arguments with technical merit. The chain of custody was unclear and consequently whether it has ever been sterilized is unknown. I was able to tactfully decline the job, pending sterilization.
Chris
----------------------------------------- The information contained in this message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately, and delete the original message.
==============================Original Headers============================== 6, 20 -- From holpc-at-firstenergycorp.com Tue Aug 28 13:15:09 2007 6, 20 -- Received: from firstenergycorp.com (gw19.firstenergycorp.com [205.132.74.180]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7SIF9QH005141 6, 20 -- for {microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 13:15:09 -0500 6, 20 -- Subject: SEM of medical device, part 2 6, 20 -- To: Microscopy-at-microscopy.com 6, 20 -- X-Mailer: Lotus Notes Release 6.5.4 CCH5 September 12, 2005 6, 20 -- Message-ID: {OF65AB6B0A.2D5F84D2-ON85257345.00620BD7-85257345.006438CE-at-FirstEnergyCorp.com} 6, 20 -- From: holpc-at-firstenergycorp.com 6, 20 -- Date: Tue, 28 Aug 2007 14:14:41 -0400 6, 20 -- MIME-Version: 1.0 6, 20 -- X-MIMETrack: Serialize by Router on mail01/Servers/FirstEnergy(Release 7.0.2FP2|May 14, 2007) at 6, 20 -- 08/28/2007 14:14:41, 6, 20 -- Itemize by SMTP Server on GW13/Servers/FirstEnergy(Release 7.0.2FP1|January 6, 20 -- 10, 2007) at 08/28/2007 02:14:42 PM, 6, 20 -- Serialize by Router on GW13/Servers/FirstEnergy(Release 7.0.2FP1|January 10, 2007) at 6, 20 -- 08/28/2007 02:14:43 PM, 6, 20 -- Serialize complete at 08/28/2007 02:14:43 PM 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="US-ASCII" ==============================End of - Headers==============================
The following procedures give the typical H & E type staining that Pathologists seem to want over the blue stain of the 0.05% Toluidine Blue O in 1% sodium borate which I use all the time now.
In my notes I have the following procedure from the 28th Annual EMSA Meeting:
A Simple Dichromatic Stain for Plastic Embedded Tissues G.R.Mackay and M.L.Mead
SOLUTION I. 0.065 grams Methylene blue 0.01 grams Azure II mixed by magnetic stirring into a solution containing: 5.0 ml Glycerol 5.0 ml 100% Methyl alcohol 40.0 ml distilled water Filter and store up to 6 months
SOLUTION II. PREPARED DAILY 50.0 ml distilled water 2.0 grams NaOH
SOLUTION III. A. 0.5% BASIC FUCHSIN Stock 100 ml water + 0.2 grams Basic Fuchsin
B. WORKING SOLUTION III - Make and Filter DAILY Into a covered Copland Jar 40.0 ml water + 10.0 ml Solution A (above)
Procedure: Place slide with section onto a hot plate at 60-80 degrees C for 2-3 min. Cool slide Create a pool of 2 parts Solution I + 1 part Solution II - do NOT stir After 5 - 10 min. rinse with running tap water Check on LM and repeat if too light Solution III - put slide in for 5 min. Rinse with water and check on LM Repeat if necessary
Corrections for over-staining:
Over-staining with Sol.I - decolorize with 100% Ethanol and re-stain for a shorter time
Counter-stain dark - soak in water filled Copland jar for 10 - 15 min. then re-stain from the start
Oil can be removed with xylene, air dry slide and re-stain
Joe Weible had taught me his "Quick Procedure" using the same solutions as above at the Wistar Institute (now at SPI) back in the early 70's
1. Put 2 drops of Sol.I + 1 drop of Sol.II onto a section that had been dried down onto a slide. Heat but do not dry, then water wash. Dip into 95% Ethanol and wash off with water. Check on scope for color. Too weak - stain again. Too dark - rinse in NaOH and water again.
2. Put 4 drops water onto slide and 1 drop Sol.III for 20 sec. on hotplate. Water wash and check color.
Regards,
Patricia Stranen Connelly Lead Technologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-65 connellyps-at-mail.nih.gov
======= On 8/28/07 12:42 PM, "Walter.Bobrowski-at-pfizer.com" {Walter.Bobrowski-at-pfizer.com} wrote:
} Could someone share some light and a protocol to achieve H&E staining on } epoxy-embedded tissue sections (heart muscle)? I simply cannot get the } hematoxylin to stain nuclei and yields a general "grayish" stain of } components. No heat yields absolutely no staining. Alcoholic eosin Y has } no problem yielding a bright pink. Maybe too well. Or this simply why I } never hear of H&E staining in EM? } Thanks! } } Walter F. Bobrowski } Sr. Scientist } Pfizer Global R&D
==============================Original Headers============================== 22, 24 -- From connellyps-at-nhlbi.nih.gov Tue Aug 28 14:02:11 2007 22, 24 -- Received: from NIHCESSMTP3.hub.nih.gov (nihcessmtp3.hub.nih.gov [128.231.90.117]) 22, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7SJ2AkJ018515 22, 24 -- for {microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 14:02:11 -0500 22, 24 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 22, 24 -- Tue, 28 Aug 2007 15:02:04 -0400 22, 24 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.166]) with Microsoft Exchange Server HTTP-DAV ; 22, 24 -- Tue, 28 Aug 2007 19:02:04 +0000 22, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618 22, 24 -- Date: Tue, 28 Aug 2007 15:00:43 -0400 22, 24 -- Subject: Re: [Microscopy] H&E Staining of Epoxy-embedded Tissues 22, 24 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 22, 24 -- To: {Walter.Bobrowski-at-pfizer.com} 22, 24 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 22, 24 -- Message-ID: {C2F9E91B.B40%connellyps-at-nhlbi.nih.gov} 22, 24 -- Thread-Topic: [Microscopy] H&E Staining of Epoxy-embedded Tissues 22, 24 -- Thread-Index: Acfppbuf+li4CVWYEdym3gANk2Yv1A== 22, 24 -- In-Reply-To: {200708281642.l7SGgDJK029661-at-ns.microscopy.com} 22, 24 -- Mime-version: 1.0 22, 24 -- Content-type: text/plain; 22, 24 -- charset="ISO-8859-1" 22, 24 -- X-OriginalArrivalTime: 28 Aug 2007 19:02:04.0838 (UTC) FILETIME=[EC66BC60:01C7E9A5] 22, 24 -- Content-Transfer-Encoding: 8bit 22, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7SJ2AkJ018515 ==============================End of - Headers==============================
It has been more than fifteen years since I taught this subject; however, if I remember correctly most programs for electron microbeam analysis report analysis results in weight fraction (i.e. grams unknown per gram total) rather than in either weight percent (gms unknown per 100 gms total) or atom percent (atoms of unknown per 100 atoms total).
I believe the basic reason for this is that the fundamental equation for the number of atoms of the unknown element that are ionized by an incident electron, per centimeter the electron travels through the specimen, is given by an equation of the form:
dn/dx = Q (N C d/A)
Where dn/dx = the number of atoms of unknown atoms ionized per cm the electron travels in the specimen, Q is the ionization cross-section of the unknown atoms, N = Avagadro's number, C = the concentration of the unknown atoms U, d = the density of the sample, and A = the atomic umber of the unknown U. To make the dimensions come out right the quantity in parentheses has to have the dimensions of (atoms per cubic centimeter), and this requires the concentration of the unknown to be expressed in weight fraction, i.e.:
{atoms U/At Wt U x gms U/gm total x gms total/ cc)/( gms U/ At Wt U) = atoms U/cc
This requirement for the use of weight fraction in the fundamental equation for the ionization process then carries through to the calculation of intensities and through all the subsequent ZAF corrections (or other machinations) used to produce the final results. Some programs may contain subroutines to convert to other units of concentration, but I think fundamentally most yield results in weight fraction.
You can find this matter discussed in more detail in various text books on the subject such as 'Scanning Electron Microscopy and X-Ray Microanalysis' by Goldstein, et. al. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 7, 14 -- From bigelow-at-umich.edu Tue Aug 28 14:28:24 2007 7, 14 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.161]) 7, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7SJSO0x030931 7, 14 -- for {microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 14:28:24 -0500 7, 14 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 7, 14 -- BY tombraider.mr.itd.umich.edu ID 46D47754.D86C9.8701 ; 7, 14 -- 28 Aug 2007 15:28:20 -0400 7, 14 -- Mime-Version: 1.0 7, 14 -- Message-Id: {p06240802c2fa1cbbc5e1-at-[141.212.131.221]} 7, 14 -- Date: Tue, 28 Aug 2007 15:28:19 -0400 7, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 7, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 7, 14 -- Subject: [Microscopy] RE: Wt % or At % 7, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Hi all, I think Michael's earlier explanation is a little confusing, especially the statement "EPMA... is more sensitive to mass% than atom%".
In fact since the column density actually defines x-ray generation volume, Jim Bigelow is right, to calculate the number of ionizations, not only weight fraction, but atomic weight, density and number of atoms must all be included for a rigorous calculation of these electronic (atomic number) dependent interactions in solid materials. However, the fact remains that these atomic level interactions (fluorescence, absorption, energy loss, etc) at these keV energies are dominated by electronic (atomic number dependent) effects, not mass effects.
X-ray excitation itself is essentially independent with respect to atomic mass (the EPMA technique is not sensitive to mass). Most atomic processes involving electron-solid interactions at energies below 100 keV are well correlated with atomic number (and electron energy) and only give the appearance (in compounds) of being more related to mass fraction than atomic fraction because A/Z is approximately a constant over the periodic table. And also involved is the historical fact that we have terms for mass (weight) fraction and atomic (number of atoms) fraction but no corresponding term related to "Z" (atomic number) fraction of compounds.
If you do not agree, simply check any table of atomic properties and note (for example) that x-ray fluorescent yields increase monotonically with Z, even in the three places in the periodic table where atomic mass decreases and atomic number increases. EPMA calculations originally started with the mass based first approximation simply because it gave a more accurate first approximation than an atomic fraction first approximation (due to the aforementioned relative constancy of A/Z) and eventually also because most of the corrections (as Jim mentioned) to the intensity ratios are based on mass normalized (e.g., mass absorption coefficients) or mass unit (e.g., stopping power) calculations.
In the case of electron backscatter loss, there is no theoretical physical basis for calculating average backscatter in compounds using mass fraction. Pure momentum exchange occurs in the case of a perfectly elastic interaction of an electron with a 180 degrees scattering angle. The largest effect is elastic scattering off a hydrogen nuclei (a 0.05% mass effect), and this effect decreases further with increasing atomic mass. And of course in most cases the scattering angle is not 180 degrees so the mass effect is even smaller.
The funny thing is that early models using mass fraction for average backscatter in compounds not only did better than atomic fraction averaging (this isn't surprising for reasons already mentioned), but mass fraction models even did better than simple Z fraction averaging because the A/Z ratio is not quite constant across the periodic table (mass increases faster than Z of course). However, this entirely unrelated effect (due to stellar nucleosynthesis s and r-processes and nuclear stability properties) just so happens to push the averaging calculation in the proper direction to serendipitously account for effects of nuclear screening by inner orbital electrons in larger atomic number atoms (which is why the backscatter curve gets flatter at higher Z- because one measures fewer than expected backscattered electrons due to screening effects). To obtain accurate predictions of average backscatter using a Z based fraction, one must also include a term for nuclear screening by inner orbital electrons.
More modern methods (e.g., monte carlo programs such as Penelope) using actual scattering cross sections implicitly take these considerations into account and are therefore even more accurate than any simple fractional based models.
At 04:53 AM 8/24/2007, you wrote: } Stephane asks ... } } } - why is the integration of the peaks in the EDX spectrum } } called "weight %"? } } - Does the intensity if the peaks in EDX depend on the atomic } } weight? In other words, do heavier elements produce more } } x-rays than lighter elements? It is a hard for me to believe } } this, because electron shells are the same for light and } } heavy elements (a K shell is a K shell), however it is the } } only reason I can see to calculate an atom% value. } } Michael says: The EPMA technique, whether EDX or WDX, is more } sensitive to mass% than } atom%. I know this is somewhat counter-intuitive and I remember having the } same conceptual problems myself. However, you can do simple tests with } stoichometric compounds that include heavy and lighter atomic numbers. A } good example would be to compare Fe metal and FeO. If you integrate the Fe } Ka peak for both you'll see that for FeO it almost directly calculates the } mass fraction rather than the atom fraction. } } Therefore mass fractions are always the direct result of EPMA, while atom } fractions are calculated secondarily. Most analysts will always report the } mass% because it will include the actual total of all elements, while the } atom% will always be a result of having normalized to 100%.
==============================Original Headers============================== 13, 21 -- From donovan-at-uoregon.edu Tue Aug 28 15:00:18 2007 13, 21 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu [128.223.142.40]) 13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7SK0INR010956 13, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 15:00:18 -0500 13, 21 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.92.75]) 13, 21 -- (authenticated bits=0) 13, 21 -- by smtp.uoregon.edu (8.13.8/8.13.8) with ESMTP id l7SK0HX0010875 13, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 13, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 13:00:17 -0700 13, 21 -- Message-Id: {200708282000.l7SK0HX0010875-at-smtp.uoregon.edu} 13, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 21 -- Date: Tue, 28 Aug 2007 12:57:10 -0700 13, 21 -- To: Microscopy-at-microscopy.com 13, 21 -- From: John Donovan {donovan-at-uoregon.edu} 13, 21 -- Subject: Re: [Microscopy] RE: SEM: wt% or atom% ? 13, 21 -- In-Reply-To: {200708241153.l7OBra30025043-at-ns.microscopy.com} 13, 21 -- References: {200708241153.l7OBra30025043-at-ns.microscopy.com} 13, 21 -- Mime-Version: 1.0 13, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 13, 21 -- X-Virus-Scanned: ClamAV 0.91.2/4088/Tue Aug 28 04:51:32 2007 on mserv1 13, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
It has been some time since I had needed to use this stain so my memory may not be accurate.
I followed Joe's way instead of filling a whole Copland jar for a few slides. After washing the slide it was cool. I put it onto the hotplate, added room temp. water and Sol. III right away so the stain never got really hot - only warm before I washed it off, about 10-15 sec. after the addition of Sol.III. This worked well.
As to the investigator wanting H&E, he seems to be coming from a medical background and expects that H&E works on everything and does not know TEM techniques. Try to get a well stained slide with the MABF Stain (Methylene blue, Azure, Basic Fuchsin) for him to look at then - 1. Politely ask if he has ever seen H&E on Epon sections.
2. If he answers YES, ask him for contact information so you can get the procedure from that person (who may have given him MABF and not told him).
3. If he answers NO, mention that there is a reason that he hasn't and that reason is that H&E does not work on plastic sections and suggest that if he really wants it done in the future to send a portion of the tissue to the Pathology/LM lab to work up when he sends TEM work to your lab. If you do both, then he has to tell you when you receive the tissue so that you can process the two portions differently.
Good luck, Pat ================= On 8/28/07 3:13 PM, "Bobrowski, Walter" {Walter.Bobrowski-at-pfizer.com} wrote:
} Thanks Patricia. I have that exact recipe (printed in Microscopy Today) } and use as a special stain, though slightly different procedure. One } quick question: is your staining done in coplin jars at RT, and NOT on a } hotplate? I have done this on a hotplate and found that the basic } fuchsin will destain the blue completely if left too long (} 20 seconds). } Please let me know! Thanks } Oh, and the investigator specifically wanted H&E staining, which doesn't } work unless you go through the effort to deplasticizing, which I'm not } about to do. } Walt } } -----Original Message----- } From: Patricia Connelly [mailto:connellyps-at-nhlbi.nih.gov]
} Walter, } } The following procedures give the typical H & E type staining that } Pathologists seem to want over the blue stain of the 0.05% Toluidine } Blue O in 1% sodium borate which I use all the time now. } } In my notes I have the following procedure from the } 28th Annual EMSA Meeting: } } A Simple Dichromatic Stain for Plastic Embedded Tissues } G.R.Mackay and M.L.Mead } } "MABF" Modification [original - Belanger, Stain Technology, 36:313 (1961)] } } SOLUTION I. } 0.065 grams Methylene blue } 0.01 grams Azure II } mixed by magnetic stirring into a solution containing: } 5.0 ml Glycerol } 5.0 ml 100% Methyl alcohol } 40.0 ml distilled water } Filter and store up to 6 months } } SOLUTION II. PREPARED DAILY } 50.0 ml distilled water } 2.0 grams NaOH } } SOLUTION III. } A. 0.5% BASIC FUCHSIN Stock } 100 ml water + 0.2 grams Basic Fuchsin } } B. WORKING SOLUTION III - Make and Filter DAILY } Into a covered Copland Jar } 40.0 ml water + 10.0 ml Solution A (above) } } Procedure: } Place slide with section onto a hot plate at 60-80 degrees C for } 2-3 min. } Cool slide } Create a pool of 2 parts Solution I + 1 part Solution II - do NOT stir } After 5 - 10 min. rinse with running tap water } Check on LM and repeat if too light } Solution III - put slide in for 5 min. } Rinse with water and check on LM } Repeat if necessary } } Corrections for over-staining: } } Over-staining with Sol.I - decolorize with 100% Ethanol and re-stain for } a shorter time. } } Counter-stain dark - soak in water filled Copland jar for 10 - 15 min. } then re-stain from the start. } } Oil can be removed with xylene, air dry slide and re-stain. } } Joe Weible had taught me his "Quick Procedure" using the same solutions } as above at the Wistar Institute (now at SPI) back in the early 70's. } } 1. Put 2 drops of Sol.I + 1 drop of Sol.II onto a section that had been } dried down onto a slide. Heat but do not dry, then water wash. } Dip into 95% Ethanol and wash off with water. Check on scope for color. } Too weak - stain again. Too dark - rinse in NaOH and water again. } } 2. Put 4 drops water onto slide and 1 drop Sol.III for 20 sec. on } hotplate. Water wash and check color. } } Regards, } } Patricia Stranen Connelly } Lead Technologist } NHLBI Electron Microscopy Core } National Institutes of Health } 14 Service Road South } Bldg. 14E - Rm. 111B MSC 5570 } Bethesda, MD 20892-5570 } Phone 301-496-3491 } FAX 301-480-65 } connellyps-at-mail.nih.gov } } ======= } On 8/28/07 12:42 PM, "Walter.Bobrowski-at-pfizer.com" } {Walter.Bobrowski-at-pfizer.com} wrote:
Could someone share some light and a protocol to achieve H&E staining on epoxy-embedded tissue sections (heart muscle)? I simply cannot get the hematoxylin to stain nuclei and yields a general "grayish" stain of components. No heat yields absolutely no staining. Alcoholic eosin Y has no problem yielding a bright pink. Maybe too well. Or this simply why I never hear of H&E staining in EM? Thanks!
Walter F. Bobrowski Sr. Scientist Pfizer Global R&D
} LEGAL NOTICE } Unless expressly stated otherwise, this message is confidential and may be } privileged. It is intended for the addressee(s) only. Access to this E-mail } by anyone else is unauthorized. If you are not an addressee, any disclosure } or copying of the contents of this E-mail or any action taken (or not taken) } in reliance on it is unauthorized and may be unlawful. If you are not an } addressee, please inform the sender immediately.
==============================Original Headers============================== 11, 23 -- From connellyps-at-nhlbi.nih.gov Tue Aug 28 15:43:06 2007 11, 23 -- Received: from NIHCESSMTP.hub.nih.gov (nihcessmtp.hub.nih.gov [128.231.90.115]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7SKh5WG023852 11, 23 -- for {microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 15:43:05 -0500 11, 23 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 11, 23 -- Tue, 28 Aug 2007 16:43:00 -0400 11, 23 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.166]) with Microsoft Exchange Server HTTP-DAV ; 11, 23 -- Tue, 28 Aug 2007 20:43:00 +0000 11, 23 -- User-Agent: Microsoft-Entourage/11.3.6.070618 11, 23 -- Date: Tue, 28 Aug 2007 16:41:39 -0400 11, 23 -- Subject: Re: [Microscopy] H&E Staining of Epoxy-embedded Tissues 11, 23 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 11, 23 -- To: "Bobrowski, Walter" {Walter.Bobrowski-at-pfizer.com} 11, 23 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 23 -- Message-ID: {C2FA00C3.B50%connellyps-at-nhlbi.nih.gov} 11, 23 -- Thread-Topic: [Microscopy] H&E Staining of Epoxy-embedded Tissues 11, 23 -- Thread-Index: Acfppbuf+li4CVWYEdym3gANk2Yv1AAAUSdQAAM1QxQ= 11, 23 -- In-Reply-To: {CF89AF6ABD93B746A55BCCD9480A4B3D09987D5A-at-anaamrexm01.amer.pfizer.com} 11, 23 -- Mime-version: 1.0 11, 23 -- Content-type: text/plain; 11, 23 -- charset="US-ASCII" 11, 23 -- Content-transfer-encoding: 7bit 11, 23 -- X-OriginalArrivalTime: 28 Aug 2007 20:43:00.0704 (UTC) FILETIME=[05FA8E00:01C7E9B4] ==============================End of - Headers==============================
A quick search on PubMed gave a list of over 60 references--many of which provide H&E type stains. I also remember a publication in the 1970s that listed many LM stains applied to sodium methoxide-etched section, but can't locate either the paper or the reference due to my recent retirement and the concomitant state of disarray of everything that goes with changing one's life. Try several PubMed searches--I didn't specifically search for H&E staining. I seem to remember the paper was in Lab Invest or some such similar journal, altho' the ones that came up in my search were J Ultrastruct Res, Stain Technology plus other oldies but goodies. I have used (in the dim, dark, distant past) a toludine blue/alcoholic basic fuchsin technique, but it was fussy and inconsistent in my hands.
Roger Moretz
On 8/28/07, connellyps-at-nhlbi.nih.gov {connellyps-at-nhlbi.nih.gov} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Walter, } } It has been some time since I had needed to use this stain so my memory may } not be accurate. } } I followed Joe's way instead of filling a whole Copland jar for a few } slides. } After washing the slide it was cool. } I put it onto the hotplate, added room temp. water and Sol. III right away } so the stain never got really hot - only warm before I washed it off, about } 10-15 sec. after the addition of Sol.III. } This worked well. } } As to the investigator wanting H&E, he seems to be coming from a medical } background and expects that H&E works on everything and does not know TEM } techniques. } Try to get a well stained slide with the MABF Stain (Methylene blue, Azure, } Basic Fuchsin) for him to look at then - } 1. Politely ask if he has ever seen H&E on Epon sections. } } 2. If he answers YES, ask him for contact information so you can get the } procedure from that person (who may have given him MABF and not told him). } } 3. If he answers NO, mention that there is a reason that he hasn't and that } reason is that H&E does not work on plastic sections and suggest that if he } really wants it done in the future to send a portion of the tissue to the } Pathology/LM lab to work up when he sends TEM work to your lab. If you do } both, then he has to tell you when you receive the tissue so that you can } process the two portions differently. } } Good luck, } Pat } ================= } On 8/28/07 3:13 PM, "Bobrowski, Walter" {Walter.Bobrowski-at-pfizer.com} wrote: } } } Thanks Patricia. I have that exact recipe (printed in Microscopy Today) } } and use as a special stain, though slightly different procedure. One } } quick question: is your staining done in coplin jars at RT, and NOT on a } } hotplate? I have done this on a hotplate and found that the basic } } fuchsin will destain the blue completely if left too long (} 20 seconds). } } Please let me know! Thanks } } Oh, and the investigator specifically wanted H&E staining, which doesn't } } work unless you go through the effort to deplasticizing, which I'm not } } about to do. } } Walt } } } } -----Original Message----- } } From: Patricia Connelly [mailto:connellyps-at-nhlbi.nih.gov] } } } Walter, } } } } The following procedures give the typical H & E type staining that } } Pathologists seem to want over the blue stain of the 0.05% Toluidine } } Blue O in 1% sodium borate which I use all the time now. } } } } In my notes I have the following procedure from the } } 28th Annual EMSA Meeting: } } } } A Simple Dichromatic Stain for Plastic Embedded Tissues } } G.R.Mackay and M.L.Mead } } } } "MABF" Modification [original - Belanger, Stain Technology, } 36:313 (1961)] } } } } SOLUTION I. } } 0.065 grams Methylene blue } } 0.01 grams Azure II } } mixed by magnetic stirring into a solution containing: } } 5.0 ml Glycerol } } 5.0 ml 100% Methyl alcohol } } 40.0 ml distilled water } } Filter and store up to 6 months } } } } SOLUTION II. PREPARED DAILY } } 50.0 ml distilled water } } 2.0 grams NaOH } } } } SOLUTION III. } } A. 0.5% BASIC FUCHSIN Stock } } 100 ml water + 0.2 grams Basic Fuchsin } } } } B. WORKING SOLUTION III - Make and Filter DAILY } } Into a covered Copland Jar } } 40.0 ml water + 10.0 ml Solution A (above) } } } } Procedure: } } Place slide with section onto a hot plate at 60-80 degrees C for } } 2-3 min. } } Cool slide } } Create a pool of 2 parts Solution I + 1 part Solution II - do NOT stir } } After 5 - 10 min. rinse with running tap water } } Check on LM and repeat if too light } } Solution III - put slide in for 5 min. } } Rinse with water and check on LM } } Repeat if necessary } } } } Corrections for over-staining: } } } } Over-staining with Sol.I - decolorize with 100% Ethanol and re-stain for } } a shorter time. } } } } Counter-stain dark - soak in water filled Copland jar for 10 - 15 min. } } then re-stain from the start. } } } } Oil can be removed with xylene, air dry slide and re-stain. } } } } Joe Weible had taught me his "Quick Procedure" using the same solutions } } as above at the Wistar Institute (now at SPI) back in the early 70's. } } } } 1. Put 2 drops of Sol.I + 1 drop of Sol.II onto a section that had been } } dried down onto a slide. Heat but do not dry, then water wash. } } Dip into 95% Ethanol and wash off with water. Check on scope for color. } } Too weak - stain again. Too dark - rinse in NaOH and water again. } } } } 2. Put 4 drops water onto slide and 1 drop Sol.III for 20 sec. on } } hotplate. Water wash and check color. } } } } Regards, } } } } Patricia Stranen Connelly } } Lead Technologist } } NHLBI Electron Microscopy Core } } National Institutes of Health } } 14 Service Road South } } Bldg. 14E - Rm. 111B MSC 5570 } } Bethesda, MD 20892-5570 } } Phone 301-496-3491 } } FAX 301-480-65 } } connellyps-at-mail.nih.gov } } } } ======= } } On 8/28/07 12:42 PM, "Walter.Bobrowski-at-pfizer.com" } } {Walter.Bobrowski-at-pfizer.com} wrote: } } Could someone share some light and a protocol to achieve H&E } staining on epoxy-embedded tissue sections (heart muscle)? } I simply cannot get the hematoxylin to stain nuclei and yields a general } "grayish" stain of components. No heat yields absolutely no staining. } Alcoholic eosin Y has no problem yielding a bright pink. Maybe too well. } Or this simply why I never hear of H&E staining in EM? } Thanks! } } Walter F. Bobrowski } Sr. Scientist } Pfizer Global R&D } } } LEGAL NOTICE } } Unless expressly stated otherwise, this message is confidential and may be } } privileged. It is intended for the addressee(s) only. Access to this E-mail } } by anyone else is unauthorized. If you are not an addressee, any disclosure } } or copying of the contents of this E-mail or any action taken (or not taken) } } in reliance on it is unauthorized and may be unlawful. If you are not an } } addressee, please inform the sender immediately. } } } ==============================Original Headers============================== } 11, 23 -- From connellyps-at-nhlbi.nih.gov Tue Aug 28 15:43:06 2007 } 11, 23 -- Received: from NIHCESSMTP.hub.nih.gov (nihcessmtp.hub.nih.gov [128.231.90.115]) } 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7SKh5WG023852 } 11, 23 -- for {microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 15:43:05 -0500 } 11, 23 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); } 11, 23 -- Tue, 28 Aug 2007 16:43:00 -0400 } 11, 23 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.166]) with Microsoft Exchange Server HTTP-DAV ; } 11, 23 -- Tue, 28 Aug 2007 20:43:00 +0000 } 11, 23 -- User-Agent: Microsoft-Entourage/11.3.6.070618 } 11, 23 -- Date: Tue, 28 Aug 2007 16:41:39 -0400 } 11, 23 -- Subject: Re: [Microscopy] H&E Staining of Epoxy-embedded Tissues } 11, 23 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} } 11, 23 -- To: "Bobrowski, Walter" {Walter.Bobrowski-at-pfizer.com} } 11, 23 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 11, 23 -- Message-ID: {C2FA00C3.B50%connellyps-at-nhlbi.nih.gov} } 11, 23 -- Thread-Topic: [Microscopy] H&E Staining of Epoxy-embedded Tissues } 11, 23 -- Thread-Index: Acfppbuf+li4CVWYEdym3gANk2Yv1AAAUSdQAAM1QxQ= } 11, 23 -- In-Reply-To: {CF89AF6ABD93B746A55BCCD9480A4B3D09987D5A-at-anaamrexm01.amer.pfizer.com} } 11, 23 -- Mime-version: 1.0 } 11, 23 -- Content-type: text/plain; } 11, 23 -- charset="US-ASCII" } 11, 23 -- Content-transfer-encoding: 7bit } 11, 23 -- X-OriginalArrivalTime: 28 Aug 2007 20:43:00.0704 (UTC) FILETIME=[05FA8E00:01C7E9B4] } ==============================End of - Headers============================== }
==============================Original Headers============================== 3, 29 -- From rcmoretz-at-gmail.com Tue Aug 28 19:53:55 2007 3, 29 -- Received: from nf-out-0910.google.com (nf-out-0910.google.com [64.233.182.185]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7T0rsBP008724 3, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 19:53:54 -0500 3, 29 -- Received: by nf-out-0910.google.com with SMTP id d3so24140nfc 3, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 17:53:53 -0700 (PDT) 3, 29 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 3, 29 -- d=gmail.com; s=beta; 3, 29 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 3, 29 -- b=fGMkldo5HbidwB4vGNhz8p+YnPMN6V5S+mpKmA28a7kqmFIsqAuekZYuv5yjyQbkpaVTs9Z4BE49z0fPwiSru4Wi1Qt5/QiiFr2b7u0Q5YpAf3KHU013fjMuehyjG/za13r4zkMYAad+5qn2XIQ4Ug9ljj58P5HL1paWw+iszTo= 3, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 29 -- d=gmail.com; s=beta; 3, 29 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 3, 29 -- b=RklxcDPgaLOilWMxzU2vzzlFx9dE3NZkD6ALjvBtHo7Te5MYTZQrb7kudAJj4P5VnYOf6LhaIsQNeSOD5vSUmj2ROKKHnmYiPFvYU31KrLkspq1QZzsJGoD1Gi4KxdO1rSUdRcgDvLRlQcccTN9lblJlmapTtxOspQywtNJPtjI= 3, 29 -- Received: by 10.78.176.20 with SMTP id y20mr33466hue.1188348833144; 3, 29 -- Tue, 28 Aug 2007 17:53:53 -0700 (PDT) 3, 29 -- Received: by 10.78.100.5 with HTTP; Tue, 28 Aug 2007 17:53:52 -0700 (PDT) 3, 29 -- Message-ID: {950e3cfd0708281753u304336dm1ca85f0ea6f9bad3-at-mail.gmail.com} 3, 29 -- Date: Tue, 28 Aug 2007 20:53:52 -0400 3, 29 -- From: "Roger Moretz" {rcmoretz-at-gmail.com} 3, 29 -- To: connellyps-at-nhlbi.nih.gov, 3, 29 -- "Microscopy Listserv" {Microscopy-at-microscopy.com} 3, 29 -- Subject: Re: [Microscopy] Re: H&E Staining of Epoxy-embedded Tissues 3, 29 -- In-Reply-To: {200708282048.l7SKmH9r031533-at-ns.microscopy.com} 3, 29 -- MIME-Version: 1.0 3, 29 -- Content-Type: text/plain; charset=ISO-8859-1 3, 29 -- Content-Transfer-Encoding: 7bit 3, 29 -- Content-Disposition: inline 3, 29 -- References: {200708282048.l7SKmH9r031533-at-ns.microscopy.com} ==============================End of - Headers==============================
Here is a protocol for H&E staining of epoxy-embedded tissues that was developed by K.A. Pasyk and C.A. Hassett, Path. Res. Pract. 184, 635-638 (1989).
1. Cover tissue sections on the slide with 4 % hydrogen peroxide (2 drops 30 % hydrogen peroxide in 48 drops dd H2O) for 4 minutes. 2. Rinse the slide with ddH2O and dry on a hot plate a few seconds. Any sections that may have loosened will again adhere to the slide. 3. Flood the slide with Gill's III hematoxylin and place in moist chamber at 37 degrees C for 90 minutes. 4. Rinse with water then flood with ammonia water (2 drops ammonium hydroxide in 100 ml dd H2O). 5. Rinse with water then flood with 1 % alcoholic eosin Y for 5 minutes. 6. Rinse in 100 % ethanol to remove excess stain. 7. Dry the slide on a hot plate at 60 degrees C then coverslip using Permount mounting medium.
I hope this helps.
Best regards,
Dotty Sorenson
On Aug 28, 2007, at 12:40 PM, Walter.Bobrowski-at-pfizer.com wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Could someone share some light and a protocol to achieve H&E } staining on } epoxy-embedded tissue sections (heart muscle)? I simply cannot get the } hematoxylin to stain nuclei and yields a general "grayish" stain of } components. No heat yields absolutely no staining. Alcoholic eosin } Y has } no problem yielding a bright pink. Maybe too well. Or this simply } why I } never hear of H&E staining in EM? } Thanks! } } } Walter F. Bobrowski } Sr. Scientist } Pfizer Global R&D } } } "The ultimate human freedom is the ability to choose one's attitude } in a } given set of circumstances." -Viktor Frankl } } ---------------------------------------------------------------------- } LEGAL NOTICE } Unless expressly stated otherwise, this message is confidential and } may be privileged. It is intended for the addressee(s) only. } Access to this E-mail by anyone else is unauthorized. If you are } not an addressee, any disclosure or copying of the contents of this } E-mail or any action taken (or not taken) in reliance on it is } unauthorized and may be unlawful. If you are not an addressee, } please inform the sender immediately. } } } ==============================Original } Headers============================== } 6, 30 -- From Walter.Bobrowski-at-pfizer.com Tue Aug 28 11:37:18 2007 } 6, 30 -- Received: from mopmsgoa01.pfizer.com (mopmsgo.pfizer.com } [148.168.100.84]) } 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id l7SGbI0T021156 } 6, 30 -- for {microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 } 11:37:18 -0500 } 6, 30 -- Received: from mopamrexc02.amer.pfizer.com } (mopamrexc02.pfizer.com [170.116.30.68]) } 6, 30 -- by mopmsgoa01.pfizer.com (8.13.7/8.13.7) with ESMTP id } l7SGbHQw001311 } 6, 30 -- for {microscopy-at-microscopy.com} ; Tue, 28 Aug 2007 } 12:37:17 -0400 } 6, 30 -- Received: from mopamrexc03.amer.pfizer.com } ([170.116.30.69]) by mopamrexc02.amer.pfizer.com with Microsoft } SMTPSVC(6.0.3790.1830); } 6, 30 -- Tue, 28 Aug 2007 12:37:17 -0400 } 6, 30 -- Received: from anaamrexm01.amer.pfizer.com } ([162.48.101.10]) by mopamrexc03.amer.pfizer.com with Microsoft } SMTPSVC(6.0.3790.1830); } 6, 30 -- Tue, 28 Aug 2007 12:37:17 -0400 } 6, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 6, 30 -- Content-class: urn:content-classes:message } 6, 30 -- MIME-Version: 1.0 } 6, 30 -- Content-Type: text/plain; } 6, 30 -- charset="us-ascii" } 6, 30 -- Subject: H&E Staining of Epoxy-embedded Tissues } 6, 30 -- Date: Tue, 28 Aug 2007 12:37:20 -0400 } 6, 30 -- Message-ID: } {CF89AF6ABD93B746A55BCCD9480A4B3D09987C85-at-anaamrexm01.amer.pfizer.com} } 6, 30 -- X-MS-Has-Attach: } 6, 30 -- X-MS-TNEF-Correlator: } 6, 30 -- Thread-Topic: H&E Staining of Epoxy-embedded Tissues } 6, 30 -- Thread-Index: AcfpkbRJyrgIgnoxTHelNq81hogE/g== } 6, 30 -- From: "Bobrowski, Walter" {Walter.Bobrowski-at-pfizer.com} } 6, 30 -- To: {microscopy-at-microscopy.com} } 6, 30 -- X-OriginalArrivalTime: 28 Aug 2007 16:37:17.0380 (UTC) } FILETIME=[B245D840:01C7E991] } 6, 30 -- X-Proofpoint-Virus-Version: vendor=fsecure } engine=4.65.5502:2.3.11,1.2.37,4.0.164 } definitions=2007-08-28_07:2007-08-28,2007-08-28,2007-08-28 } signatures=0 } 6, 30 -- X-Proofpoint-Spam-Reason: safe } 6, 30 -- Content-Transfer-Encoding: 8bit } 6, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id l7SGbI0T021156 } ==============================End of - } Headers============================== } }
Dorothy Sorenson Microscopy and Image-analysis Laboratory Department of Cell and Developmental Biology University Of Michigan Medical School A807 BSRB 109 Zina Pitcher Place Ann Arbor, MI 48109-2200 (734)763-1170 FAX (734)763-1166
==============================Original Headers============================== 11, 19 -- From dsoren-at-umich.edu Wed Aug 29 07:36:52 2007 11, 19 -- Received: from skycaptain.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.160]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7TCaqRl003674 11, 19 -- for {microscopy-at-msa.microscopy.com} ; Wed, 29 Aug 2007 07:36:52 -0500 11, 19 -- Received: FROM [10.21.129.251] (host-18.subnet-17.med.umich.edu [141.214.17.18]) 11, 19 -- BY skycaptain.mr.itd.umich.edu ID 46D56827.CD168.3016 ; 11, 19 -- 29 Aug 2007 08:35:51 -0400 11, 19 -- In-Reply-To: {200708281640.l7SGepDG026958-at-ns.microscopy.com} 11, 19 -- References: {200708281640.l7SGepDG026958-at-ns.microscopy.com} 11, 19 -- Mime-Version: 1.0 (Apple Message framework v752.2) 11, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 11, 19 -- Message-Id: {1D67F188-7FF4-478A-BC96-591ECC50EC4C-at-umich.edu} 11, 19 -- Cc: microscopy-at-msa.microscopy.com 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- From: Dorothy Sorenson {dsoren-at-umich.edu} 11, 19 -- Subject: Re: [Microscopy] H&E Staining of Epoxy-embedded Tissues 11, 19 -- Date: Wed, 29 Aug 2007 08:32:07 -0400 11, 19 -- To: Walter.Bobrowski-at-pfizer.com 11, 19 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (amartine-at-ee.ucr.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, August 28, 2007 at 20:59:12 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both amartine-at-ee.ucr.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: amartine-at-ee.ucr.edu Name: Alfredo A. Martinez-Morales
Organization: University of California, Riverside
Education: Graduate College
Location: Riverside, CA 92525
Title: Inquiry about International Scientific Instrument SEM (DS-130 S)
Question: My research group at the University of California, Riverside received several years ago a SEM from the Navy. Our SEM is ISI DS130 model. I am trying to determine whether this SEM is of any value to our group, but unfortunately I have not been able to find someone who can put it together and tested to see if it still works. I was hoping someone could point me into the right direction in trying to find someone who is knowledgeable with ISI SEMs. Any information that you can provide is truly appreciated. Thank you for your time and attention.
Alfredo; We have an amazing service engineer from MAS working on our Topcon TEM who tells us that he works on DS-130's a lot, and my guess would be that if he can't fix you DS-130, nobody can. I don't know if you can contract Richard Murphy at 888-798-1867 or if you need to contact his manager, Art McCanna at 800-421-8451.
John Mardinly Intel
This is not an opinion of Intel Corporation.
-----Original Message----- X-from: amartine-at-ee.ucr.edu [mailto:amartine-at-ee.ucr.edu] Sent: Wednesday, August 29, 2007 6:26 AM To: Mardinly, John
This Question was submitted to Ask-A-Microscopist by (amartine-at-ee.ucr.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, August 28, 2007 at 20:59:12 Remember to consider the Grade/Age of the student when considering the Question ------------------------------------------------------------------------ --- Please reply to both amartine-at-ee.ucr.edu as well as to the Microscopy Listserver ------------------------------------------------------------------------ ---
Email: amartine-at-ee.ucr.edu Name: Alfredo A. Martinez-Morales
Organization: University of California, Riverside
Education: Graduate College
Location: Riverside, CA 92525
Title: Inquiry about International Scientific Instrument SEM (DS-130 S)
Question: My research group at the University of California, Riverside received several years ago a SEM from the Navy. Our SEM is ISI DS130 model. I am trying to determine whether this SEM is of any value to our group, but unfortunately I have not been able to find someone who can put it together and tested to see if it still works. I was hoping someone could point me into the right direction in trying to find someone who is knowledgeable with ISI SEMs. Any information that you can provide is truly appreciated. Thank you for your time and attention.
Kenneth R. Lawless, of Charlottesville, died at Norfolk General Hospital on Friday, August 24, 2007, from respiratory failure following a sudden, on-set lung infection.
I met Ken in the late 1960's at the Electron Microscopy Society of America meetings. For those new to the society, it used to have "Electron" in its title. He was Treasurer for EMSA seemingly forever. I always remember his reports at the EMSA business meetings as they were "short and sweet". He was one of the most enthusiastic persons about teaching microscopy that I ever met. He was like an encyclopedia BUT he made it all entertaining. He epitomized my expectations of a "southern gentleman" in all areas of his life. Besides microscopy, he loved nature, music and lots of other interests. We shall dearly miss him.
I am enclosing the obituary that appeared in the local paper.
He was born August 21, 1922, the son of the late Elma P. and William R. Lawless of Key West, Florida. He was preceded in death by his wife, Alois Y. Lawless of Charlottesville.
Kenneth is survived by his four children all of the Charlottesville/Albemarle area, daughter, Nancy Lee Kozub, son, Stephen C. Yowell and his wife, Susan, and their sons, L. Gordon Yowell II and his wife, Melissa, of Hurt, Virginia, Lindsay S. Yowell of Charlottesville, son, Kenneth W. Lawless, and his daughter, Lelia Anne Lawless and her husband, Steve Hamilton, and their four children, Sarah Hamilton, Cassidy Hamilton, Kenneth Hamilton, and Jessica Glackin and her husband, Robert, of Orange Park, Florida, and their two children Hannah and Andruw; and his sister, Virginia "Madge" DeLuca of Connecticut.
Spending most of his childhood in Lynchburg, Virginia, Kenneth received a B.S. in Chemistry and Physics from Lynchburg College in 1946, having also served nearly four years in the Army Air Corps, obtaining the rank of Captain. Following graduate work at the University of California, Los Angeles, he returned to Charlottesville and received his Ph.D. in Chemistry from the University of Virginia in 1951. He received a Fulbright Fellowship and spent a year conducting research at Norway's Institute of Technology, Trondheim, Normandy. Returning to Charlottesville, Kenneth went on to serve as a research scientist and professor at the University of Virginia for over 40 years, retiring in 1992 with the honor of Professor Emeritus status.
Among his many other contributions to the University as a researcher and professor, Kenneth was instrumental in creating the Department of Materials Science and Engineering, eventually serving as Chairman for 10 years. The department rose steadily to national and international prominence. A consummate teacher and brilliant scientist, Dr. Lawless was greatly admired by students and faculty alike. His innate love of learning was coupled with a keen desire to explore and discover the wonders of the natural world. His enthusiasm was contagious as he mentored dozens and dozens of Ph.D. candidates over the years. Former students returned from around the country to honor and pay him tribute when he retired.
Kenneth was an internationally recognized authority in the field of Electron Microscopy, primarily the study of the molecular structure of materials. Over the years, he served as guest lecturer and presenter in Japan, China, Australia and Europe. He authored and coauthored numerous papers, articles and textual contributions. Recognized widely for his expertise and scholarship, Kenneth went on to hold many offices both professional and honorary. Of particular note, he served as President of the Virginia Academy of Sciences, and Councillor, Treasurer, and later, Member Emeritus, of the Microscopy Society of America.
In 1989, Dr. Lawless was selected for membership in the "Fellows of the Virginia Academy of Sciences" - a body of scholars selected because of their outstanding contributions to scientific research, teaching and leadership. Among many other awards and commendations, Kenneth received the distinguished J. Shelton Horseley Research Award, the highest honor bestowed by the Virginia Academy of Sciences for original research. He also received the Morton D. Maser Distinguished Service Award from the Microscopy Society of America in 1992. He was a member of Alpha Chi Sigma chemistry fraternity, SigmaXl, Phi Beta Kappa, and the Raven Society. At various times, Kenneth served on the Fulbright Fellowship Selection Committee, and the University of Virginia Medical School Admissions Committee.
In addition to his research and teaching at the University of Virginia, Kenneth was widely known throughout the Commonwealth as an ornithologist, field botanist, and nature photographer. His knowledge of birds and Virginia wildflowers was legendary. An ardent naturalist and long-term member of the Virginia Society of Ornithology and the Virginia Native Plant Society, he was a mainstay participant in the Charlottesville area Christmas Bird Count for the last 60 years. Kenneth was also known for his outstanding photography of wildflowers, conducting exhibits and lectures at the National Arboretum, the Nature Conservancy, the Wintergreen Wildflower Symposium, and Camp Jeep. He routinely conducted nature walks throughout central Virginia and also served on the Flora of Virginia Project. In 2004, Kenneth was selected to serve on the Albemarle County Biodiversity Work Group, organized in part to protect and preserve the wildlife in the County.
Kenneth was a music lover all of his life. He sang with the University Singers, the Oratorio Society, the AAUW Gilbert and Sullivan operettas, and the Chancel Choir at First United Methodist Church. With his beautiful tenor voice, he was a guest soloist and ensemble participant at many area churches and other venues. Despite his extensive contributions to The University and the Commonwealth, Kenneth always maintained that the most important things to him were the love of his family and friends. In the midst of an extensive teaching schedule and frequent travel, it was rare for him to miss one of his children's piano or dance recitals, or Little League baseball games. He took great joy in introducing school children to the wonders of nature and was equally as effective in engaging second graders as well as second-year grad students. Brilliant and modest, principled and compassionate, Kenneth Lawless modeled a life well-lived and he blessed and enriched the lives of all he encountered.
==============================Original Headers============================== 16, 17 -- From murphyjudy-at-comcast.net Wed Aug 29 13:26:25 2007 16, 17 -- Received: from alnrmhc15.comcast.net (alnrmhc15.comcast.net [206.18.177.55]) 16, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7TIQPoL015181 16, 17 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Aug 2007 13:26:25 -0500 16, 17 -- Received: from [192.168.1.6] (c-76-29-164-138.hsd1.ca.comcast.net[76.29.164.138]) 16, 17 -- by comcast.net (alnrmhc15) with ESMTP 16, 17 -- id {20070829182624b1500a3bi7e} ; Wed, 29 Aug 2007 18:26:24 +0000 16, 17 -- Message-ID: {46D5BA50.3070505-at-comcast.net} 16, 17 -- Date: Wed, 29 Aug 2007 11:26:24 -0700 16, 17 -- From: Judy Murphy {murphyjudy-at-comcast.net} 16, 17 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 16, 17 -- X-Accept-Language: en-us, en 16, 17 -- MIME-Version: 1.0 16, 17 -- To: Microscopy {Microscopy-at-microscopy.com} 16, 17 -- Subject: Kenneth Robert Lawless 16, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The Center for the Study of Gene Structure and Function of Hunter College of the City University of New York is seeking a talented and dedicated technician for a position in the Digital Bio-Imaging Facility. Job responsibilities include maintenance of confocal and wide field microscopes, assisting in research, training users, and billing for use of the facility. To be considered for this post an applicant should have a background in the biological sciences, with some experience in microscopy. More advanced training in image analysis techniques and confocal microscopy can be provided. Computer expertise is a plus, as is experience with electron microscopy. The position available is at the technician level, salary will be in the range $35,000 to $43,000, commensurate with experience and will include a full benefits package. The position is available immediately. The successful applicant will work with a group of highly successful researchers at Hunter College. E-mail resume to techjob-at-genectr.hunter.cuny.edu
Dr. Lloyd Williams Dept of Biology Hunter College 695 Park Ave New York, NY 10021
==============================Original Headers============================== 3, 20 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Wed Aug 29 13:32:11 2007 3, 20 -- Received: from genectr.hunter.cuny.edu (genectr.hunter.cuny.edu [146.95.150.34]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7TIWAA9023251 3, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Aug 2007 13:32:11 -0500 3, 20 -- Content-class: urn:content-classes:message 3, 20 -- MIME-Version: 1.0 3, 20 -- Content-Type: text/plain; 3, 20 -- charset="US-ASCII" 3, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 3, 20 -- Subject: Microscopy/Imaging Technician Job Posting 3, 20 -- Date: Wed, 29 Aug 2007 14:32:48 -0400 3, 20 -- Message-ID: {DD9D9EF525BDB444A46962D3D4F6E9A501D220A6-at-xchange2.bio.hunter.cuny.edu} 3, 20 -- X-MS-Has-Attach: 3, 20 -- X-MS-TNEF-Correlator: 3, 20 -- Thread-Topic: Microscopy/Imaging Technician Job Posting 3, 20 -- Thread-Index: AcfqatWzbSTA+FEbQoqAZTR1g1z+PQ== 3, 20 -- From: "Lloyd Williams" {Williams-at-GENECTR.HUNTER.CUNY.EDU} 3, 20 -- To: {Microscopy-at-microscopy.com} 3, 20 -- Content-Transfer-Encoding: 8bit 3, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7TIWAA9023251 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both graceyen8-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: graceyen8-at-yahoo.com Name: grace yen
Organization: QHRI
Title-Subject: [Filtered] dark field image capture and analysis help needed
Question: I am looking for any help in locating an imaging system for capturing and analyzing streaming videos and stills of live blood before and after treatment for research analysis in particular measuring density and motility changes and a system that will capture the field of view of the microscope. I have an olympus cx31 with df and phase condenser, currently a 0.45 c-mount with sony ccd attached that magnifies the field and df images contain much noise and is not clear. thank you.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Jill.Verlander-at-medicine.ufl.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I suggest you contact Chuck Humphrey of Scanning Solutions, Inc.,in Orlando, FL. He is expert in caring for the DS-130. We had a DS-130C for years, and ultimately had to move it out because we needed the space for another TEM. Chuck was our ISI service engineer, then he started his own company after ISI/Topcon dropped their service division. We continued to have him service our scope as an independent and he always knew exactly what to do, not only to fix the scope, but also to optimize the performance. He's also great at training individuals to get the most out of the DS-130. He purchased our DS-130C and moved it to his facility. I haven't spoken to him in a couple of years, but you should be able to reach him at cchumph-at-aatglobal.net or (407) 234-0676.
Best of luck, Jill
Jill Verlander Reed, DVM Associate Scientist Division of Nephrology, Hypertension, and Renal Transplantation Director, College of Medicine Electron Microscopy Core Facility P.O. Box 100224 HSC 1600 SW Archer Road Room RB-167 Gainesville, FL 32610-0224
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/WWWMSAWelcome.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rra-at-stowers-institute.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rra-at-stowers-institute.org Name: Rhonda Allen
Organization: Stowers Institute
Title-Subject: [Filtered] Lipids in c. elegans
Question: I am looking to visualize lipid droplets in c. elegans and keep them looking black. Has anyone tried methods as described by Boshier, 1984, in Stain Technology using 1% p-phenylenediamine in 70% ethanol during dehydration in order to prevent extraction and keep them black?
Or another method I am interested in trying is Tannic Acid-p-phenylenediamine as described by Guyton and Klemp in The Journal of Histochemistry and Cytochemistry, 1988?
I would like to keep them black and be able to see the mono or double membrane.
These articles are fairly old, so am wondering about the relivancy today. We have currently tried High Pressure Freezing with Freeze Substitution in 2% Osmium/0.1% Uranyl Acetate/Acetone. The results are varying colors of lipid droplets, that we would like to be able to see the membranes more clearly, and they are looking for the text book black lipid droplet.
I will be adding 5% water to the next batch of freeze substituted samples, but this will not keep the lipids from being extracted by subsequent processing.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sujoy.hazra-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sujoy.hazra-at-gmail.com Name: Sujoy S. Hazra
Organization: UOW
Title-Subject: [Filtered] Electroplishing and FESEM image
Question: I was wondering if anyone has an idea of the conditions in terms of Voltage, time , temp. and flow rate for eletroplishing ECAP-ed IF steel using standard A2 electrolyte and Letropol-5 (Struers).
Also, how the surface generally looks like after electropolishing in FESEM after huge cold deformation, e.g. for the above case.
Hi, Sorry for the cross posting. I have a problem that I need help in figuring out. First, instrument parameters: JEOL JXA 8600: 5 WDS's, EDS, CL, SEI, BEI WDS 1: GFPC: TAP, PET, LDE1, LDEC WDS 2: Xe: LIF, PET WDS 3: Xe: LIF, PET WDS 4: Xe: LIF, PET WDS 5: GFPC: TAP, LDEB
20 kV, 10 nA, 10 um spot Astimex 53 mineral Std grain mount Astimex 44 Metal Std mount
Element Table: Na TAP WDS5 Mg TAP WDS5 Al TAP WDS5 Si TAP WDS5 K PET WDS2 Ca PET WDS2 Ti LIF WDS4 Fe LIF WDS4 Cs PET WDS3
Geller Software: dSpec, dQuant, dPict, etc
A grad student is doing feldspar analyses. We are not using WDS1 right now because it needs optimization and it is on the 'to-do' list. Student is trying to get a handle on accuracy and precision. Using Astimex std PlagAn65 and measuring it as an unknown. PHA's on the elements in the table are optimized, all elements calibrated. Everything 'appears' to be normal.
Problem: Analysis 1: Totals are good (97-99%) Individual concentrations are good. Analysis 2: Totals are bad (21%) Individuals are OK except Si and Al - way off! Analysis 3: Totals are good (97-99%) Individual concentrations are good. Analysis 4: Totals are bad (21%) Individuals are OK except Si and Al - way off! Analysis 5: Totals are good (97-99%) Individual concentrations are good. Analysis 6: Totals are bad (21%) Individuals are OK except Si and Al - way off!
On the 'bad' analyses, the reported conctration on Al and Si are consistent across the three runs. It simply looks like the instrument can't find the peak. I can't find anything wrong.
Have any of you ever seen anything like this before?
TIA Mike ******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html ********************************************************************
==============================Original Headers============================== 13, 18 -- From mmcheath-at-syr.edu Thu Aug 30 07:20:37 2007 13, 18 -- Received: from SUEXCL-02.ad.syr.edu (suexbe-02.ad.syr.edu [128.230.108.46]) 13, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7UCKaPY013134 13, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Aug 2007 07:20:37 -0500 13, 18 -- Received: from 128.230.24.156 ([128.230.24.156]) by SUEXCL-02.ad.syr.edu ([128.230.108.53]) via Exchange Front-End Server exchange.syr.edu ([128.230.108.50]) with Microsoft Exchange Server HTTP-DAV ; 13, 18 -- Thu, 30 Aug 2007 12:20:36 +0000 13, 18 -- User-Agent: Microsoft-Entourage/11.3.3.061214 13, 18 -- Date: Thu, 30 Aug 2007 08:20:35 -0400 13, 18 -- Subject: Loss of counts on Al and Si 13, 18 -- From: Michael Cheatham {mmcheath-at-syr.edu} 13, 18 -- To: {Microscopy-at-microscopy.com} 13, 18 -- Message-ID: {C2FC2E53.16FA7%mmcheath-at-syr.edu} 13, 18 -- Thread-Topic: Loss of counts on Al and Si 13, 18 -- Thread-Index: AcfrACqQaTtQ61bzEdyDIQAWy6AryQ== 13, 18 -- Mime-version: 1.0 13, 18 -- Content-type: text/plain; 13, 18 -- charset="US-ASCII" 13, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
-----Original Message----- X-from: nephrol-bounces-at-mailman.srv.ualberta.ca [mailto:nephrol-bounces-at-mailman.srv.ualberta.ca] On Behalf Of Nauman Tarif,MD Sent: Friday, August 24, 2007 11:30 AM To: nephrol-at-mailman.srv.ualberta.ca
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both R.I.Han-at-sms.ed.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: R.I.Han-at-sms.ed.ac.uk Name: Richard Han
Organization: University of Edinburgh
Title-Subject: [Filtered] Analyze SEM images
Question: Hi All
I would like to analyze SEM images of vascular connective tissue using ImageJ. I need some advice on how to go about in this type of analysis. Any suggestions on analyzing parameters? and how's it done? I remember someone has mentioned the SEM roughness and grey scale variation plugins before, but haven't managed to find them.
This Question was submitted to Ask-A-Microscopist by (alvarobq-at-fcien.edu.uy) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, August 30, 2007 at 13:05:08 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both alvarobq-at-fcien.edu.uy as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: I¥m using Quetol 651 for a first time. I had bad results. The blocks (in gelatin capsules) are very soft. Data sheet says what I must alter the ratio of QUETOL 651 and NSA (NSA is not the hardener)but What must I increase? NSA or QUETOL? If you have experience with this epoxi resin, please, send me a improved protocol. Data sheet suggest gelatin capsules for embedding, may I cure the resin in flats embedding molds? Thank you very much.
Hope you are doing well, This reference might be of interest to you. I have tried the technique discussed in the paper, it is quite straightforward, you equilibrate the sample in an imidazole buffer and add osmium to the solution, I have got really good lipid staining with the technique.
Here is the reference.
"Imidazole-buffered osmium tetroxide: an excellent stain for visualization of lipids in transmission electron microscopy" Authors: Sabine Angermuller and Dariush Fahimi. Histochem J. 1982 Sep;14(5):823-35
Neeraj V. Gohad Graduate Research Assistant Department of Biological Sciences Clemson University, Clemson, SC-29634 Phone: 864-656-3597 Fax: 864-656-0435
-----Original Message----- X-from: rra-at-stowers-institute.org [mailto:rra-at-stowers-institute.org] Sent: Wednesday, August 29, 2007 6:11 PM To: neerajg-at-CLEMSON.EDU
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/WWWMSAWelcome.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying
please copy both rra-at-stowers-institute.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rra-at-stowers-institute.org Name: Rhonda Allen
Organization: Stowers Institute
Title-Subject: [Filtered] Lipids in c. elegans
Question: I am looking to visualize lipid droplets in c. elegans and keep them looking black. Has anyone tried methods as described by Boshier, 1984, in Stain Technology using 1% p-phenylenediamine in 70% ethanol during dehydration in order to prevent extraction and keep them black?
Or another method I am interested in trying is Tannic Acid-p-phenylenediamine as described by Guyton and Klemp in The Journal of Histochemistry and Cytochemistry, 1988?
I would like to keep them black and be able to see the mono or double membrane.
These articles are fairly old, so am wondering about the relivancy today. We have currently tried High Pressure Freezing with Freeze Substitution in 2% Osmium/0.1% Uranyl Acetate/Acetone. The results are varying colors of lipid droplets, that we would like to be able to see the membranes more clearly, and they are looking for the text book black lipid droplet.
I will be adding 5% water to the next batch of freeze substituted samples, but this will not keep the lipids from being extracted by subsequent processing.
Have any of you run into the current bane of canned air that includes anti-inhaling ingredients? It is supposed to prevent "huffing." In so doing, the cans basically deny all use for what is normal for these. Like cleaning mirrors, specimen holders, cover slips, etc. The most recent is Falcon Dust Off. Useless.
Are there normal, historical canned air products still available? Do these need a background check and a ten day waiting period to purchase?
This is ridiculous.
gary g.
==============================Original Headers============================== 6, 17 -- From gary-at-gaugler.com Thu Aug 30 19:19:19 2007 6, 17 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l7V0JJFN023770 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 30 Aug 2007 19:19:19 -0500 6, 17 -- Message-Id: {200708310019.l7V0JJFN023770-at-ns.microscopy.com} 6, 17 -- Received: (qmail 23935 invoked from network); 30 Aug 2007 17:19:19 -0700 6, 17 -- Received: by simscan 1.1.0 ppid: 23926, pid: 23928, t: 0.1436s 6, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 6, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 6, 17 -- by qsmtp2 with SMTP; 30 Aug 2007 17:19:19 -0700 6, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 6, 17 -- Date: Thu, 30 Aug 2007 17:19:23 -0800 6, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 6, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 6, 17 -- Subject: Useless canned air 6, 17 -- Mime-Version: 1.0 6, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-21832694 ==============================End of - Headers==============================
Does anybody have an extra TV scan module for a JSM-35C? I really want to be able to capture images via a frame grabber on a computer to be able to print them out for students, and this seems the most economical method of doing so for the short term until grant writing gets underway. I know that the resolution won't be that good, but it doesn't matter- we just need to print out pictures of things that the students can bring home and show their parents without blowing our entire budget on Polaroid film.
Of course, if anybody has an old QuartzPCI or Orion system hanging around that would digitize our scope, that would be preferable...
--Justin A. Kraft Leadership Academy West
==============================Original Headers============================== 3, 26 -- From kraftpiano-at-gmail.com Thu Aug 30 21:24:31 2007 3, 26 -- Received: from wa-out-1112.google.com (wa-out-1112.google.com [209.85.146.176]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7V2OVsB005266 3, 26 -- for {microscopy-at-microscopy.com} ; Thu, 30 Aug 2007 21:24:31 -0500 3, 26 -- Received: by wa-out-1112.google.com with SMTP id v27so795573wah 3, 26 -- for {microscopy-at-microscopy.com} ; Thu, 30 Aug 2007 19:24:30 -0700 (PDT) 3, 26 -- DKIM-Signature: a=rsa-sha1; c=relaxed/relaxed; 3, 26 -- d=gmail.com; s=beta; 3, 26 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 26 -- b=YLGH/P2PGapd6c0XjLTHzUi2Orp1GmlRQBOwaQOCPCDCaisikNxVbtkvlBTRtYb/RJ2MV6ChA5pUxvYsIopZlcdN2xU1CRKm5SEL/5MZdbgjmweO9w6OXRXkfLepQToSIbJk8qTEYnLCExcybC+tgAT+j7tp/6Jb2AW3IE6tmpg= 3, 26 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 26 -- d=gmail.com; s=beta; 3, 26 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 26 -- b=AgWMeBwRgno4njHfzyix1bHHEfuN5pvM9KJFibtepKiHGrO6jLC3n46hMyuGpyW/JBWx7gmWbEodeOGmB0Gss5CqKKTklZb+m+lEpZ8Ap+6laLLEEpAN6lRJP/BP9f7ae0hWLQ2kaBuOSbcCfuyPBCfRdnki16Cif7KZ3t86+DU= 3, 26 -- Received: by 10.114.149.2 with SMTP id w2mr367261wad.1188527070221; 3, 26 -- Thu, 30 Aug 2007 19:24:30 -0700 (PDT) 3, 26 -- Received: by 10.114.92.5 with HTTP; Thu, 30 Aug 2007 19:24:30 -0700 (PDT) 3, 26 -- Message-ID: {25e2b0d20708301924r688e4892l5f83b32de2007bb3-at-mail.gmail.com} 3, 26 -- Date: Thu, 30 Aug 2007 22:24:30 -0400 3, 26 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 26 -- To: microscopy-at-microscopy.com 3, 26 -- Subject: TV Scan module for a JSM-35C 3, 26 -- MIME-Version: 1.0 3, 26 -- Content-Type: text/plain; charset=ISO-8859-1 3, 26 -- Content-Transfer-Encoding: 7bit 3, 26 -- Content-Disposition: inline ==============================End of - Headers==============================
Unfortunately, I can't help directly. Was not aware that the "dusters" are being corrupted. However, thanks for the heads-up.
For the most part, I quit using them a number of years ago. I added a little plumbing in the lab and distribute compressed nitrogen to quick-disconnects scattered about the lab. Plug in the nozzle valve/hose and voila! I do regulate down the pressure a bit ;)
Woody
-----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: Thursday, August 30, 2007 8:20 PM To: White, Woody N.
Hi all:
Have any of you run into the current bane of canned air that includes anti-inhaling ingredients? It is supposed to prevent "huffing." In so doing, the cans basically deny all use for what is normal for these. Like cleaning mirrors, specimen holders, cover slips, etc. The most recent is Falcon Dust Off. Useless.
Are there normal, historical canned air products still available? Do these need a background check and a ten day waiting period to purchase?
This is ridiculous.
gary g.
==============================Original Headers============================== 6, 17 -- From gary-at-gaugler.com Thu Aug 30 19:19:19 2007 6, 17 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l7V0JJFN023770 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 30 Aug 2007 19:19:19 -0500 6, 17 -- Message-Id: {200708310019.l7V0JJFN023770-at-ns.microscopy.com} 6, 17 -- Received: (qmail 23935 invoked from network); 30 Aug 2007 17:19:19 -0700 6, 17 -- Received: by simscan 1.1.0 ppid: 23926, pid: 23928, t: 0.1436s 6, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 6, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 6, 17 -- by qsmtp2 with SMTP; 30 Aug 2007 17:19:19 -0700 6, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 6, 17 -- Date: Thu, 30 Aug 2007 17:19:23 -0800 6, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 6, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 6, 17 -- Subject: Useless canned air 6, 17 -- Mime-Version: 1.0 6, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-21832694 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 28 -- From nwwhite-at-bwxt.com Fri Aug 31 07:05:18 2007 18, 28 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7VC5EZr027341 18, 28 -- for {microscopy-at-msa.microscopy.com} ; Fri, 31 Aug 2007 07:05:16 -0500 18, 28 -- Received: from ([131.184.13.224]) 18, 28 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.5874850; 18, 28 -- Fri, 31 Aug 2007 08:04:52 -0400 18, 28 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); 18, 28 -- Fri, 31 Aug 2007 08:04:51 -0400 18, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 18, 28 -- Content-class: urn:content-classes:message 18, 28 -- MIME-Version: 1.0 18, 28 -- Content-Type: text/plain; 18, 28 -- charset="us-ascii" 18, 28 -- Subject: RE: [Microscopy] Useless canned air 18, 28 -- Date: Fri, 31 Aug 2007 08:04:51 -0400 18, 28 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB7F879B4-at-BWXSPO01.BWXS.BWXTECH.NET} 18, 28 -- In-Reply-To: {200708310019.l7V0Jvft024516-at-ns.microscopy.com} 18, 28 -- X-MS-Has-Attach: 18, 28 -- X-MS-TNEF-Correlator: 18, 28 -- Thread-Topic: [Microscopy] Useless canned air 18, 28 -- Thread-Index: AcfrZLQJmQ0cQWbJSWC+DkS+olwplgAYX9Sg 18, 28 -- References: {200708310019.l7V0Jvft024516-at-ns.microscopy.com} 18, 28 -- From: "White, Woody N." {NWWhite-at-bwxt.com} 18, 28 -- To: {gary-at-gaugler.com} , "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 18, 28 -- X-OriginalArrivalTime: 31 Aug 2007 12:04:51.0975 (UTC) FILETIME=[22E43970:01C7EBC7] 18, 28 -- Content-Transfer-Encoding: 8bit 18, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7VC5EZr027341 ==============================End of - Headers==============================
Gary, I concur with Woody. I too gave up on canned air 1 year ago and now have a coiled air-line with pistol-type release valve dangling at my workstation, attached to a tank of N2 (in an out of the way storage closet) with a valve at the workstation to regulate pressure. Works great! Walt
Hello Gary,
Unfortunately, I can't help directly. Was not aware that the "dusters" are being corrupted. However, thanks for the heads-up.
For the most part, I quit using them a number of years ago. I added a little plumbing in the lab and distribute compressed nitrogen to quick-disconnects scattered about the lab. Plug in the nozzle valve/hose and voila! I do regulate down the pressure a bit ;)
Woody
---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately.
==============================Original Headers============================== 11, 30 -- From Walter.Bobrowski-at-pfizer.com Fri Aug 31 07:43:17 2007 11, 30 -- Received: from mopmsgoa01.pfizer.com (mopmsgo.pfizer.com [148.168.100.84]) 11, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7VChGCB007254 11, 30 -- for {microscopy-at-microscopy.com} ; Fri, 31 Aug 2007 07:43:16 -0500 11, 30 -- Received: from mopamrexc01.amer.pfizer.com (mopamrexc01.pfizer.com [170.116.32.254]) 11, 30 -- by mopmsgoa01.pfizer.com (8.13.7/8.13.7) with ESMTP id l7VCh5Un019028 11, 30 -- for {microscopy-at-microscopy.com} ; Fri, 31 Aug 2007 08:43:16 -0400 11, 30 -- Received: from groamrexc01.amer.pfizer.com ([172.30.8.168]) by mopamrexc01.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.1830); 11, 30 -- Fri, 31 Aug 2007 08:43:14 -0400 11, 30 -- Received: from anaamrexm01.amer.pfizer.com ([162.48.101.10]) by groamrexc01.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.1830); 11, 30 -- Fri, 31 Aug 2007 08:43:13 -0400 11, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 30 -- Content-class: urn:content-classes:message 11, 30 -- MIME-Version: 1.0 11, 30 -- Content-Type: text/plain; 11, 30 -- charset="us-ascii" 11, 30 -- Subject: RE: [Microscopy] RE: Useless canned air 11, 30 -- Date: Fri, 31 Aug 2007 08:43:11 -0400 11, 30 -- Message-ID: {CF89AF6ABD93B746A55BCCD9480A4B3D099DE1B8-at-anaamrexm01.amer.pfizer.com} 11, 30 -- X-MS-Has-Attach: 11, 30 -- X-MS-TNEF-Correlator: 11, 30 -- Thread-Topic: RE: [Microscopy] RE: Useless canned air 11, 30 -- Thread-Index: AcfryQMOxHsA+Y7GRaaic4cww8wVAAAApP7Q 11, 30 -- From: "Bobrowski, Walter" {Walter.Bobrowski-at-pfizer.com} 11, 30 -- To: {microscopy-at-microscopy.com} 11, 30 -- X-OriginalArrivalTime: 31 Aug 2007 12:43:13.0503 (UTC) FILETIME=[7EB586F0:01C7EBCC] 11, 30 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=4.65.5502:2.3.11,1.2.37,4.0.164 definitions=2007-08-31_01:2007-08-28,2007-08-31,2007-08-31 signatures=0 11, 30 -- X-Proofpoint-Spam-Reason: safe 11, 30 -- Content-Transfer-Encoding: 8bit 11, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l7VChGCB007254 ==============================End of - Headers==============================
I recommend using clean house nitrogen; no cleaner source of gas exists. This is exceptionally easy if you have access to house nitrogen supplied by your facility. Alternatively, place nitrogen outlets with around your lab plumbed to 1A nitrogen cylinders.
Regards,'
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Happiness equals reality minus expectations." Tom Magliozzi
gary-at-gaugler.c om To gary.m.brown-at-exxonmobil.com 08/30/07 07:21 cc PM Subject [Microscopy] Useless canned air Please respond to gary-at-gaugler.c om
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi all:
Have any of you run into the current bane of canned air that includes anti-inhaling ingredients? It is supposed to prevent "huffing." In so doing, the cans basically deny all use for what is normal for these. Like cleaning mirrors, specimen holders, cover slips, etc. The most recent is Falcon Dust Off. Useless.
Are there normal, historical canned air products still available? Do these need a background check and a ten day waiting period to purchase?
This is ridiculous.
gary g.
==============================Original Headers============================== 6, 17 -- From gary-at-gaugler.com Thu Aug 30 19:19:19 2007 6, 17 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l7V0JJFN023770 6, 17 -- for {microscopy-at-microscopy.com} ; Thu, 30 Aug 2007 19:19:19 -0500 6, 17 -- Message-Id: {200708310019.l7V0JJFN023770-at-ns.microscopy.com} 6, 17 -- Received: (qmail 23935 invoked from network); 30 Aug 2007 17:19:19 -0700 6, 17 -- Received: by simscan 1.1.0 ppid: 23926, pid: 23928, t: 0.1436s 6, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 6, 17 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 6, 17 -- by qsmtp2 with SMTP; 30 Aug 2007 17:19:19 -0700 6, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 6, 17 -- Date: Thu, 30 Aug 2007 17:19:23 -0800 6, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 6, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 6, 17 -- Subject: Useless canned air 6, 17 -- Mime-Version: 1.0 6, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-21832694 ==============================End of - Headers==============================
==============================Original Headers============================== 27, 19 -- From gary.m.brown-at-exxonmobil.com Fri Aug 31 08:58:41 2007 27, 19 -- Received: from hoespc01.exxonmobil.com (hoespc01.exxonmobil.com [158.35.223.1]) 27, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7VDwfKj020384 27, 19 -- for {microscopy-at-microscopy.com} ; Fri, 31 Aug 2007 08:58:41 -0500 27, 19 -- Received: from dalnmg04.na.xom.com (dalnmg04.na.xom.com [131.126.97.123]) 27, 19 -- by hoespc01.exxonmobil.com (Switch-3.1.11/Switch-3.1.11) with ESMTP id l7VDwabh028259; 27, 19 -- Fri, 31 Aug 2007 08:58:39 -0500 (CDT) 27, 19 -- In-Reply-To: {200708310021.l7V0LPE4026945-at-ns.microscopy.com} 27, 19 -- Subject: Re: [Microscopy] Useless canned air 27, 19 -- Importance: 27, 19 -- To: gary-at-gaugler.com, microscopy-at-microscopy.com 27, 19 -- X-Mailer: Lotus Notes 652HF109 January 14, 2005 27, 19 -- Message-ID: {OF25BB575C.FE9114B2-ON86257348.004C36A3-86257348.004CC68F-at-exxonmobil.com} 27, 19 -- From: gary.m.brown-at-exxonmobil.com 27, 19 -- Date: Fri, 31 Aug 2007 08:58:36 -0500 27, 19 -- X-MIMETrack: Serialize by Router on Dalnmg04.na.xom.com/S/ExxonMobil(652FP1HF193|March 27, 19 -- 02, 2006) at 08/31/2007 08:58:39 AM 27, 19 -- MIME-Version: 1.0 27, 19 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Chuck is indeed an expert with the DS130. He maintains ours, and has since he installed it in 1986. He is the person to call for help with the DS130. Not to disparage anyone else - just agreeing that Chuck is can repair your microscope if it is repairable at all.
His current e-mail address is: cchumph-at-bellsouth.net
Regards, Andrew
At 05:02 PM 8/29/2007, Jill.Verlander-at-medicine.ufl.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 8, 26 -- From werner-at-rosharon.oilfield.slb.com Fri Aug 31 15:01:58 2007 8, 26 -- Received: from us1061mta02.mail.slb.com (usxsl050.slb.atosorigin-asp.com [199.6.139.15]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7VK1vQr006991 8, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 31 Aug 2007 15:01:58 -0500 8, 26 -- Received: from us1061mta02.mail.slb.com (localhost.localdomain [127.0.0.1]) 8, 26 -- by localhost (Postfix) with SMTP id 5E54A9C1FF 8, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 31 Aug 2007 20:01:57 +0000 (GMT) 8, 26 -- Received: from usxsl052.slb.atosorigin-asp.com (usxsl052dmz.slb.atosorigin-asp.com [199.6.139.167]) 8, 26 -- by us1061mta02.mail.slb.com (Postfix) with ESMTP id 1E93B9C1F3 8, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 31 Aug 2007 20:01:57 +0000 (GMT) 8, 26 -- Received: from WERNER1-OFS1.rosharon.oilfield.slb.com ([163.188.47.162]) 8, 26 -- by us085mbx01.slb.atosorigin-asp.com 8, 26 -- (Sun Java System Messaging Server 6.2-8.04 (built Feb 28 2007)) 8, 26 -- with ESMTPSA id {0JNN006SKMB0YI70-at-us085mbx01.slb.atosorigin-asp.com} for 8, 26 -- Microscopy-at-microscopy.com; Fri, 31 Aug 2007 20:01:48 +0000 (GMT) 8, 26 -- Date: Fri, 31 Aug 2007 15:01:48 -0500 8, 26 -- From: Andrew Werner {werner-at-rosharon.oilfield.slb.com} 8, 26 -- Subject: Re: [Microscopy] viaWWW: ISI DS-130C service 8, 26 -- In-reply-to: {200708292202.l7TM2nCh014491-at-ns.microscopy.com} 8, 26 -- To: Microscopy-at-microscopy.com 8, 26 -- Message-id: {7.0.1.0.2.20070831145152.03357ab8-at-rosharon.oilfield.slb.com} 8, 26 -- MIME-version: 1.0 8, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 8, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 8, 26 -- Content-transfer-encoding: 7BIT 8, 26 -- References: {200708292202.l7TM2nCh014491-at-ns.microscopy.com} ==============================End of - Headers==============================
The idea of nitrogen gas is good, but may run afoul of the safety people. about 20 years ago I convinced a new department chair that the cost and inconvenience of the air cans dictated a change. Safety people were very iffy about the use of Nitrogen, they felt it presented a safety risk for oxygen deprivation from rooms it would be used in. They suggested using the building's compressed air system. All we did was put a filter in the line to remove oil and particulates - needed it for our Airfuge anyway. Now I have three plug-ins around the lab, a coiled line and a pistol with different nozzles that came from the local auto supply store. Works great, no fees for nitrogen, or tank rentals.
Paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-392
==============================Original Headers============================== 5, 21 -- From paul_hazelton-at-umanitoba.ca Fri Aug 31 16:28:00 2007 5, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7VLRxni020007 5, 21 -- for {microscopy-at-microscopy.com} ; Fri, 31 Aug 2007 16:28:00 -0500 5, 21 -- Received: from [192.168.100.101] (wnpgmb01dc2-107-236.dynamic.mts.net [142.161.107.236]) 5, 21 -- (authenticated bits=0) 5, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id l7VLRwmY002113 5, 21 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NO); 5, 21 -- Fri, 31 Aug 2007 16:27:59 -0500 (CDT) 5, 21 -- Message-ID: {46D887EA.5010304-at-umanitoba.ca} 5, 21 -- Date: Fri, 31 Aug 2007 16:28:10 -0500 5, 21 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca} 5, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 5, 21 -- X-Accept-Language: en-us, en 5, 21 -- MIME-Version: 1.0 5, 21 -- To: gary-at-gaugler.com, Microscopy Listserver {microscopy-at-microscopy.com} 5, 21 -- Subject: Re: [Microscopy] Useless canned air 5, 21 -- References: {200708310021.l7V0LQrL026965-at-ns.microscopy.com} 5, 21 -- In-Reply-To: {200708310021.l7V0LQrL026965-at-ns.microscopy.com} 5, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
MicroscopyListserver Archive Email Extraction Software Version NJZ07060908