I cannot imagine the "safety czars" being more concerned about oxygen deprivation from a nitrogen tank, when we already have a dozen nitrogen, argon, and helium cylinders in the lab.
Even with the best filtered in-house compressed air, I would never trust it over tanked N2 extra-dry, prepure, or commercial.
Just my two cents..........
JQuinn
} From mail-at-ns.microscopy.com Fri Aug 31 17:28:44 2007 } Date: Fri, 31 Aug 2007 16:28:35 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: paul_hazelton-at-umanitoba.ca } Reply-to: paul_hazelton-at-umanitoba.ca } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] Re: Useless canned air } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Gary et al } } The idea of nitrogen gas is good, but may run afoul of the safety } people. about 20 years ago I convinced a new department chair that the } cost and inconvenience of the air cans dictated a change. Safety people } were very iffy about the use of Nitrogen, they felt it presented a } safety risk for oxygen deprivation from rooms it would be used in. They } suggested using the building's compressed air system. All we did was } put a filter in the line to remove oil and particulates - needed it for } our Airfuge anyway. Now I have three plug-ins around the lab, a coiled } line and a pistol with different nozzles that came from the local auto } supply store. Works great, no fees for nitrogen, or tank rentals. } } Paul } } } Paul R. Hazelton, PhD } Electron Microscope Unit } University of Manitoba } Department of Medical Microbiology } 531 Basic Medical Sciences Building } 730 William Avenue } Winnipeg, Manitoba, Canada, R3E 0W3 } e-mail: paul_hazelton-at-umanitoba.ca } Phone:204-789-3313 } Pager:204-931-9354 } Cell:204-781-1502 } Fax:204-789-392 } } ==============================Original Headers============================== } 5, 21 -- From paul_hazelton-at-umanitoba.ca Fri Aug 31 16:28:00 2007 } 5, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) } 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l7VLRxni020007 } 5, 21 -- for {microscopy-at-microscopy.com} ; Fri, 31 Aug 2007 16:28:00 -0500 } 5, 21 -- Received: from [192.168.100.101] (wnpgmb01dc2-107-236.dynamic.mts.net [142.161.107.236]) } 5, 21 -- (authenticated bits=0) } 5, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id l7VLRwmY002113 } 5, 21 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NO); } 5, 21 -- Fri, 31 Aug 2007 16:27:59 -0500 (CDT) } 5, 21 -- Message-ID: {46D887EA.5010304-at-umanitoba.ca} } 5, 21 -- Date: Fri, 31 Aug 2007 16:28:10 -0500 } 5, 21 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca} } 5, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) } 5, 21 -- X-Accept-Language: en-us, en } 5, 21 -- MIME-Version: 1.0 } 5, 21 -- To: gary-at-gaugler.com, Microscopy Listserver {microscopy-at-microscopy.com} } 5, 21 -- Subject: Re: [Microscopy] Useless canned air } 5, 21 -- References: {200708310021.l7V0LQrL026965-at-ns.microscopy.com} } 5, 21 -- In-Reply-To: {200708310021.l7V0LQrL026965-at-ns.microscopy.com} } 5, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 5, 21 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 16, 12 -- From jquinn-at-www.matscieng.sunysb.edu Sat Sep 1 11:57:46 2007 16, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 16, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l81GvjCm021679 16, 12 -- for {microscopy-at-microscopy.com} ; Sat, 1 Sep 2007 11:57:46 -0500 16, 12 -- Received: (from jquinn-at-localhost) 16, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id l81GvjQ17348 16, 12 -- for microscopy-at-microscopy.com; Sat, 1 Sep 2007 12:57:45 -0400 16, 12 -- Date: Sat, 1 Sep 2007 12:57:45 -0400 16, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 16, 12 -- Message-Id: {200709011657.l81GvjQ17348-at-www.matscieng.sunysb.edu} 16, 12 -- To: microscopy-at-microscopy.com 16, 12 -- Subject: re: oxygen deprivation ==============================End of - Headers==============================
All, Our new underground analytical facility is going on-line next month:
http://giving.uoregon.edu/i/facilities_index.php
We are using ultra dry compressed air to feed a nitrogen generator that will provide 12 liters/minutes of 99.995 N2 (model 96-97NA). Cost about $15K, but no more N2 bottles to lug around! I realize that 12 liters per minutes isn't all that impressive, but we will have a fairly large piping system volume as a reservoir. Mostly this will be used as a dry vent gas, but some instruments want an oxygen free vent, so we have to go this route.
If one runs the 12 lpm calculation in a normal size room, there's not much danger. Nevertheless we are installing flammable gas and oxygen level sensors since we can't simply open a window. They aren't that expensive. john
At 10:05 AM 9/1/2007, you wrote:
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==============================Original Headers============================== 8, 17 -- From donovan-at-uoregon.edu Sat Sep 1 12:39:55 2007 8, 17 -- Received: from rwcrmhc12.comcast.net (rwcrmhc12.comcast.net [216.148.227.152]) 8, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l81Hds4U001585 8, 17 -- for {microscopy-at-microscopy.com} ; Sat, 1 Sep 2007 12:39:54 -0500 8, 17 -- Message-Id: {200709011739.l81Hds4U001585-at-ns.microscopy.com} 8, 17 -- Received: from source.uoregon.edu (c-76-105-219-235.hsd1.or.comcast.net[76.105.219.235]) 8, 17 -- by comcast.net (rwcrmhc12) with SMTP 8, 17 -- id {20070901173954m1200dgbuse} ; Sat, 1 Sep 2007 17:39:54 +0000 8, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 17 -- Date: Sat, 01 Sep 2007 10:39:54 -0700 8, 17 -- To: microscopy-at-microscopy.com 8, 17 -- From: "John J. Donovan" {donovan-at-uoregon.edu} 8, 17 -- Subject: Re: [Microscopy] re: oxygen deprivation 8, 17 -- In-Reply-To: {200709011705.l81H5b81030330-at-ns.microscopy.com} 8, 17 -- References: {200709011705.l81H5b81030330-at-ns.microscopy.com} 8, 17 -- Mime-Version: 1.0 8, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
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Hello, Today morning when our Philips CM100 was started after weekend "Stand-by" the starting procedure stopped at Compustage calibration step. The message on OPCON: "MESSAGE: Remove Specimen Holder and Press READY" However, when the READY button was pressed, the error beep was generated and compustage calibrating procedure did not continue. Other microscope function are not affected. Please, could anybody give us any advice or hint? Thanking you in advance Oldrich
------------------------------ Oldrich Benada Institute of Microbiology v.v.i. Acad. Sci. CR Videnska 1083 142 20 Prague 4 Czech Republic
==============================Original Headers============================== 3, 23 -- From benada-at-biomed.cas.cz Mon Sep 3 02:01:28 2007 3, 23 -- Received: from biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8371RLv020499 3, 23 -- for {microscopy-at-microscopy.com} ; Mon, 3 Sep 2007 02:01:28 -0500 3, 23 -- Received: from [147.231.44.101] (u117ob.mbu.cas.cz [147.231.44.101]) 3, 23 -- by biomed.cas.cz (8.13.4+Sun/8.13.3) with ESMTP id l8371Qc5019753 3, 23 -- for {microscopy-at-microscopy.com} ; Mon, 3 Sep 2007 09:01:26 +0200 (CEST) 3, 23 -- From: "Oldrich Benada" {benada-at-biomed.cas.cz} 3, 23 -- Organization: Institute of Microbiology 3, 23 -- To: microscopy-at-microscopy.com 3, 23 -- Date: Mon, 03 Sep 2007 09:01:24 +0200 3, 23 -- MIME-Version: 1.0 3, 23 -- Subject: Compustage Problem 3, 23 -- Message-ID: {46DBCD64.11846.11BAB2-at-benada.biomed.cas.cz} 3, 23 -- X-Confirm-Reading-To: "Oldrich Benada" {benada-at-biomed.cas.cz} 3, 23 -- X-pmrqc: 1 3, 23 -- Priority: normal 3, 23 -- X-mailer: Pegasus Mail for Windows (4.41) 3, 23 -- Content-type: text/plain; charset=US-ASCII 3, 23 -- Content-transfer-encoding: 7BIT 3, 23 -- Content-description: Mail message body 3, 23 -- X-Antivirus: avast! (VPS 000771-2, 02.09.2007), Outbound message 3, 23 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
I can. I've run into more than one safety czar who would express exactly the concern that Paul mentions. I had an interesting enough time here just with the idea of carrying a 1 liter flask of LN2 to a classroom where it was needed. Recall that there are two fundamental kinds of safety hazards: those that involve potentially dangerous chemicals and equipment, and are safety risks, and those that involve bureaucrats and are administrative risks. Not necessarily the same thing.
But, more to the point: nitrogen lines and compressed gas lines are good ideas, but only where they either already exist or there is the budget to install them. So, for those of us who have neither of those, does anyone have an answer to Gary's original question? Are there good, clean-enough-for-optics, cans of compressed air/gas?
Phil
} Folks } } I cannot imagine the "safety czars" being more concerned about } oxygen deprivation from a nitrogen tank, when we already have } a dozen nitrogen, argon, and helium cylinders in the lab. } } Even with the best filtered in-house compressed air, I would never } trust it over tanked N2 extra-dry, prepure, or commercial. } } Just my two cents.......... } } JQuinn } } } Gary et al } } } } The idea of nitrogen gas is good, but may run afoul of the safety } } people. about 20 years ago I convinced a new department chair that the } } cost and inconvenience of the air cans dictated a change. Safety people } } were very iffy about the use of Nitrogen, they felt it presented a } } safety risk for oxygen deprivation from rooms it would be used in. They } } suggested using the building's compressed air system. All we did was } } put a filter in the line to remove oil and particulates - needed it for } } our Airfuge anyway. Now I have three plug-ins around the lab, a coiled } } line and a pistol with different nozzles that came from the local auto } } supply store. Works great, no fees for nitrogen, or tank rentals. } } } } Paul } } } } } } Paul R. Hazelton, PhD } } Electron Microscope Unit } } University of Manitoba } } Department of Medical Microbiology } } 531 Basic Medical Sciences Building } } 730 William Avenue } } Winnipeg, Manitoba, Canada, R3E 0W3 } } e-mail: paul_hazelton-at-umanitoba.ca } } Phone:204-789-3313 } } Pager:204-931-9354 } } Cell:204-781-1502 } } Fax:204-789-392 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859
==============================Original Headers============================== 4, 22 -- From oshel1pe-at-cmich.edu Tue Sep 4 07:17:16 2007 4, 22 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l84CHFcf008386 4, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Sep 2007 07:17:16 -0500 4, 22 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 22 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id l84Cd0Y2006993 4, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Sep 2007 08:39:01 -0400 4, 22 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 4, 22 -- Tue, 4 Sep 2007 08:16:49 -0400 4, 22 -- Mime-Version: 1.0 4, 22 -- Message-Id: {f06240803c302fb920f9c-at-[141.209.160.249]} 4, 22 -- In-Reply-To: {200709011704.l81H4FKA028357-at-ns.microscopy.com} 4, 22 -- References: {200709011704.l81H4FKA028357-at-ns.microscopy.com} 4, 22 -- Date: Tue, 4 Sep 2007 08:17:10 -0400 4, 22 -- To: Microscopy-at-microscopy.com 4, 22 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 22 -- Subject: [Microscopy] re: oxygen deprivation 4, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 22 -- X-OriginalArrivalTime: 04 Sep 2007 12:16:50.0076 (UTC) FILETIME=[7890BDC0:01C7EEED] 4, 22 -- X-CanItPRO-Stream: default 4, 22 -- X-Spam-Score: -4 () L_EXCH_MF 4, 22 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
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Email: mckee-at-helix.mgh.harvard.edu Name: Mary McKee
Organization: MGH
Title-Subject: [Filtered] AMT CCD camera service contract
Question: We have an AMT camera on our JEOL TEM and are trying to decide whether or not to get a service contract on it. In 2 years, there have been no problems with it. Does anyone out there care to comment on it's reliability longer term? Please reply to me off the list.
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Email: katie.gibbs-at-grace.com Name: Katie Gibbs
Title-Subject: [Filtered] Biological Preperation Classes for SEM
Question: Can anyone recommend a class or course in biological sample preparation for the SEM. I have been working with mostly inorganic materials for the last 7 years.
As a piece of answer to the original question from Gary, as Phil has remind us, I frequently use an insufflator rubber bulb to clean up samples, one which takes the air at it rear side, and blow it at the front side. The separate in and out valves make one don't suck in the bulb the dust one want to blow up from the sample. Of coarse, one blow with air, which is as clean and dry as the room... With a little nose at the output, the air jet is fine and and can be soft or strong (depends how stormy one squash the bulb !). Not class 10 clean room certified, but for current lab work, it does the job, is inexpensive and I have no trouble with myself, as I wear too a safety czar hat, here !
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
oshel1pe-at-cmich.edu a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I can. } I've run into more than one safety czar who would express exactly the } concern that Paul mentions. I had an interesting enough time here } just with the idea of carrying a 1 liter flask of LN2 to a classroom } where it was needed. } Recall that there are two fundamental kinds of safety hazards: those } that involve potentially dangerous chemicals and equipment, and are } safety risks, and those that involve bureaucrats and are } administrative risks. Not necessarily the same thing. } } But, more to the point: nitrogen lines and compressed gas lines are } good ideas, but only where they either already exist or there is the } budget to install them. So, for those of us who have neither of } those, does anyone have an answer to Gary's original question? Are } there good, clean-enough-for-optics, cans of compressed air/gas? } } Phil } } } } Folks } } } } I cannot imagine the "safety czars" being more concerned about } } oxygen deprivation from a nitrogen tank, when we already have } } a dozen nitrogen, argon, and helium cylinders in the lab. } } } } Even with the best filtered in-house compressed air, I would never } } trust it over tanked N2 extra-dry, prepure, or commercial. } } } } Just my two cents.......... } } } } JQuinn } } } } } Gary et al } } } } } } The idea of nitrogen gas is good, but may run afoul of the safety } } } people. about 20 years ago I convinced a new department chair that the } } } cost and inconvenience of the air cans dictated a change. Safety people } } } were very iffy about the use of Nitrogen, they felt it presented a } } } safety risk for oxygen deprivation from rooms it would be used in. They } } } suggested using the building's compressed air system. All we did was } } } put a filter in the line to remove oil and particulates - needed it for } } } our Airfuge anyway. Now I have three plug-ins around the lab, a coiled } } } line and a pistol with different nozzles that came from the local auto } } } supply store. Works great, no fees for nitrogen, or tank rentals. } } } } } } Paul } } } } } } } } } Paul R. Hazelton, PhD } } } Electron Microscope Unit } } } University of Manitoba } } } Department of Medical Microbiology } } } 531 Basic Medical Sciences Building } } } 730 William Avenue } } } Winnipeg, Manitoba, Canada, R3E 0W3 } } } e-mail: paul_hazelton-at-umanitoba.ca } } } Phone:204-789-3313 } } } Pager:204-931-9354 } } } Cell:204-781-1502 } } } Fax:204-789-392 } }
==============================Original Headers============================== 8, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Sep 4 10:21:23 2007 8, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.156]) 8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l84FLMYs015386 8, 29 -- for {microscopy-at-microscopy.com} ; Tue, 4 Sep 2007 10:21:22 -0500 8, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 8, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id l84FLJpZ049376 8, 29 -- for {microscopy-at-microscopy.com} ; Tue, 4 Sep 2007 17:21:19 +0200 (CEST) 8, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 8, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 7647E3EC096 8, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 4 Sep 2007 17:20:21 +0200 (CEST) 8, 29 -- Message-ID: {46DD77BE.5010609-at-ipcms.u-strasbg.fr} 8, 29 -- Date: Tue, 04 Sep 2007 17:20:30 +0200 8, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 8, 29 -- User-Agent: Thunderbird 1.5.0.13 (X11/20070824) 8, 29 -- MIME-Version: 1.0 8, 29 -- To: Microscopy-at-microscopy.com 8, 29 -- Subject: Re: [Microscopy] re: oxygen deprivation 8, 29 -- References: {200709041226.l84CQ6na017354-at-ns.microscopy.com} 8, 29 -- In-Reply-To: {200709041226.l84CQ6na017354-at-ns.microscopy.com} 8, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 29 -- Content-Transfer-Encoding: 8bit 8, 29 -- X-IPCMS-MailScanner: Found to be clean 8, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 8, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.156]); Tue, 04 Sep 2007 17:21:19 +0200 (CEST) 8, 29 -- X-Virus-Scanned: ClamAV 0.88.7/4152/Tue Sep 4 16:16:36 2007 on mr6.u-strasbg.fr 8, 29 -- X-Virus-Status: Clean 8, 29 -- X-Spam-Status: No, score=-0.1 required=5.0 tests=AWL autolearn=disabled 8, 29 -- version=3.1.8 8, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr6.u-strasbg.fr ==============================End of - Headers==============================
I know this isn't the perfect solution, but I have a 5-gal "portable air tank" (available from NAPA auto, and other sources for about $35) with added fittings for filling from a N2 cylinder and the pistol-style fine tip blow-gun for dispensing. It has a built-in gage and valve. It should give good clean gas if compressed N2 is all you ever put into it.
Link for the NAPA item (sorry it is very long...) http://www.napaonline.com/MasterPages/NOLMaster.aspx?PageId=470&LineCode=BK&PartNumber=8211350&Description=Air+Tank+%2f+Portable
I have no commercial interest in NAPA (or NASCAR)
Dale Callaham
jacques.faerber-at-ipcms.u-strasbg.fr wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all } } As a piece of answer to the original question from Gary, as Phil has } remind us, I frequently use an insufflator rubber bulb to clean up } samples, one which takes the air at it rear side, and blow it at the } front side. The separate in and out valves make one don't suck in the } bulb the dust one want to blow up from the sample. Of coarse, one blow } with air, which is as clean and dry as the room... With a little nose at } the output, the air jet is fine and and can be soft or strong (depends } how stormy one squash the bulb !). Not class 10 clean room certified, } but for current lab work, it does the job, is inexpensive and I have no } trouble with myself, as I wear too a safety czar hat, here ! } } Hope it helps } } J. Faerber } IPCMS-GSI } (Institut de Physique et Chimie des Matériaux de Strasbourg } Groupe Surface et Interfaces) } 23, rue de Loess ; BP43 } 67034 Strasbourg CEDEX 2 } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)3 88 10 72 48 } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr } } } } oshel1pe-at-cmich.edu a écrit : } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } I can. } } I've run into more than one safety czar who would express exactly the } } concern that Paul mentions. I had an interesting enough time here } } just with the idea of carrying a 1 liter flask of LN2 to a classroom } } where it was needed. } } Recall that there are two fundamental kinds of safety hazards: those } } that involve potentially dangerous chemicals and equipment, and are } } safety risks, and those that involve bureaucrats and are } } administrative risks. Not necessarily the same thing. } } } } But, more to the point: nitrogen lines and compressed gas lines are } } good ideas, but only where they either already exist or there is the } } budget to install them. So, for those of us who have neither of } } those, does anyone have an answer to Gary's original question? Are } } there good, clean-enough-for-optics, cans of compressed air/gas? } } } } Phil } } } } } } } Folks } } } } } } I cannot imagine the "safety czars" being more concerned about } } } oxygen deprivation from a nitrogen tank, when we already have } } } a dozen nitrogen, argon, and helium cylinders in the lab. } } } } } } Even with the best filtered in-house compressed air, I would never } } } trust it over tanked N2 extra-dry, prepure, or commercial. } } } } } } Just my two cents.......... } } } } } } JQuinn } } } } } } } Gary et al } } } } } } } } The idea of nitrogen gas is good, but may run afoul of the safety } } } } people. about 20 years ago I convinced a new department chair that the } } } } cost and inconvenience of the air cans dictated a change. Safety people } } } } were very iffy about the use of Nitrogen, they felt it presented a } } } } safety risk for oxygen deprivation from rooms it would be used in. They } } } } suggested using the building's compressed air system. All we did was } } } } put a filter in the line to remove oil and particulates - needed it for } } } } our Airfuge anyway. Now I have three plug-ins around the lab, a coiled } } } } line and a pistol with different nozzles that came from the local auto } } } } supply store. Works great, no fees for nitrogen, or tank rentals. } } } } } } } } Paul } } } } } } } } } } } } Paul R. Hazelton, PhD } } } } Electron Microscope Unit } } } } University of Manitoba } } } } Department of Medical Microbiology } } } } 531 Basic Medical Sciences Building } } } } 730 William Avenue } } } } Winnipeg, Manitoba, Canada, R3E 0W3 } } } } e-mail: paul_hazelton-at-umanitoba.ca } } } } Phone:204-789-3313 } } } } Pager:204-931-9354 } } } } Cell:204-781-1502 } } } } Fax:204-789-392 } } } } } ==============================Original Headers============================== } 8, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Sep 4 10:21:23 2007 } 8, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.156]) } 8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l84FLMYs015386 } 8, 29 -- for {microscopy-at-microscopy.com} ; Tue, 4 Sep 2007 10:21:22 -0500 } 8, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) } 8, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id l84FLJpZ049376 } 8, 29 -- for {microscopy-at-microscopy.com} ; Tue, 4 Sep 2007 17:21:19 +0200 (CEST) } 8, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) } 8, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 7647E3EC096 } 8, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 4 Sep 2007 17:20:21 +0200 (CEST) } 8, 29 -- Message-ID: {46DD77BE.5010609-at-ipcms.u-strasbg.fr} } 8, 29 -- Date: Tue, 04 Sep 2007 17:20:30 +0200 } 8, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} } 8, 29 -- User-Agent: Thunderbird 1.5.0.13 (X11/20070824) } 8, 29 -- MIME-Version: 1.0 } 8, 29 -- To: Microscopy-at-microscopy.com } 8, 29 -- Subject: Re: [Microscopy] re: oxygen deprivation } 8, 29 -- References: {200709041226.l84CQ6na017354-at-ns.microscopy.com} } 8, 29 -- In-Reply-To: {200709041226.l84CQ6na017354-at-ns.microscopy.com} } 8, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 29 -- Content-Transfer-Encoding: 8bit } 8, 29 -- X-IPCMS-MailScanner: Found to be clean } 8, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr } 8, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.156]); Tue, 04 Sep 2007 17:21:19 +0200 (CEST) } 8, 29 -- X-Virus-Scanned: ClamAV 0.88.7/4152/Tue Sep 4 16:16:36 2007 on mr6.u-strasbg.fr } 8, 29 -- X-Virus-Status: Clean } 8, 29 -- X-Spam-Status: No, score=-0.1 required=5.0 tests=AWL autolearn=disabled } 8, 29 -- version=3.1.8 } 8, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr6.u-strasbg.fr } ==============================End of - Headers==============================
-- } {(((º} L L } {(((º} .·´¯`·.¸ {º)))} { ¸.·´¯`·.¸ } {(((º} ¸.·´¯¯¯¯¯¯¯
Dale A. Callaham Central Microscopy Facility The University of Massachusetts Amherst, MA 01003
-- Along this same line of thought, does anyone know of any in-room sensor that can be purchased that emits an alarm if oxygen levels get low. I am thinking of something that works like either a smoke alarm or a carbon monoxide detector. We need to use ethane gas for our cryo procedures and the safety people are causing an uproar about that. These people tend to make a "one size fits all" type of regulation that often has little relevance to specific situations. If anyone has any specific product I would appreciate the info.
Norm Olson
______________________________________________________________ Norm Olson Cryoelectron Microscopy Facilities Manager 1510 Bonner Hall Department of Chemistry & Biochemistry, MC-0378 University of California San Diego La Jolla, CA 92093-0378 nholson-at-ucsd.edu http://cryoem.ucsd.edu Cell: (858)220-2183 (858)822-6718 - Office; (858)534-5846 - Fax ______________________________________________________________
Back when we still had a darkroom with rotary doors, the safety people asked us to put in extra heavy duty hoses for the nitrogen guns that we used to blow off dust. We thought they were going overboard at first when they chose 12,000 psi rated brake hoses, but this was judged much cheaper than oxygen sensors, and when we thought about the scenario they were designed to prevent, we thought it was OK. What is the scenario? Imagine a hose fails in the middle of the night or weekend. The room fills with nitrogen, displacing all of the air. A person entering the darkroom through the rotary doors would be breathing 100% nitrogen, and would pass out after taking one breath. Nobody on the outside would see them. Anyone entering the darkroom would suffer the same fate. These would become fatalities. Basically, the consequences of a nitrogen leak were deemed so severe that 12,000 psi rated brake hose nitrogen lines seemed reasonable when used in darkroom with rotary doors.
John Mardinly Intel
This is not an opinion of Intel Corporation.
-----Original Message----- X-from: nholson-at-ucsd.edu [mailto:nholson-at-ucsd.edu] Sent: Tuesday, September 04, 2007 12:42 PM To: Mardinly, John
-- Along this same line of thought, does anyone know of any in-room sensor that can be purchased that emits an alarm if oxygen levels get low. I am thinking of something that works like either a smoke alarm or a carbon monoxide detector. We need to use ethane gas for our cryo procedures and the safety people are causing an uproar about that. These people tend to make a "one size fits all" type of regulation that often has little relevance to specific situations. If anyone has any specific product I would appreciate the info.
Norm Olson
______________________________________________________________ Norm Olson Cryoelectron Microscopy Facilities Manager 1510 Bonner Hall Department of Chemistry & Biochemistry, MC-0378 University of California San Diego La Jolla, CA 92093-0378 nholson-at-ucsd.edu http://cryoem.ucsd.edu Cell: (858)220-2183 (858)822-6718 - Office; (858)534-5846 - Fax ______________________________________________________________
We are a commercial testing laboratory and We use nitrogen lines (have a hook up coming from the plant supply) to Run some of our equipment and also to blow off samples in our metallography area. This works out a lot better for us due to the fact that the plant air lines sometimes get water in them. We are talking about getting quite a substantial stream of water out of an air line, instead of just air. Not good!
The thing we have done for safety, is to install Oxygen sensors in the vicinity of these nitrogen supply lines. And these sensors are connected to Very LOUD Alarm and large red flashing lights outside of the rooms where nitrogen supplies are present. The alarms are set to go off if it registers oxygen less than around 19%or20%. The sensors are serviced on a regular basis.
Peace, Kelly A. Ramos
-----Original Message----- X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com] Sent: Tuesday, September 04, 2007 4:30 PM To: Ramos, Kelly A
Back when we still had a darkroom with rotary doors, the safety people asked us to put in extra heavy duty hoses for the nitrogen guns that we used to blow off dust. We thought they were going overboard at first when they chose 12,000 psi rated brake hoses, but this was judged much cheaper than oxygen sensors, and when we thought about the scenario they were designed to prevent, we thought it was OK. What is the scenario? Imagine a hose fails in the middle of the night or weekend. The room fills with nitrogen, displacing all of the air. A person entering the darkroom through the rotary doors would be breathing 100% nitrogen, and would pass out after taking one breath. Nobody on the outside would see them. Anyone entering the darkroom would suffer the same fate. These would become fatalities. Basically, the consequences of a nitrogen leak were deemed so severe that 12,000 psi rated brake hose nitrogen lines seemed reasonable when used in darkroom with rotary doors.
John Mardinly Intel
This is not an opinion of Intel Corporation.
-----Original Message----- X-from: nholson-at-ucsd.edu [mailto:nholson-at-ucsd.edu] Sent: Tuesday, September 04, 2007 12:42 PM To: Mardinly, John
-- Along this same line of thought, does anyone know of any in-room sensor that can be purchased that emits an alarm if oxygen levels get low. I am thinking of something that works like either a smoke alarm or a carbon monoxide detector. We need to use ethane gas for our cryo procedures and the safety people are causing an uproar about that. These people tend to make a "one size fits all" type of regulation that often has little relevance to specific situations. If anyone has any specific product I would appreciate the info.
Norm Olson
______________________________________________________________ Norm Olson Cryoelectron Microscopy Facilities Manager 1510 Bonner Hall Department of Chemistry & Biochemistry, MC-0378 University of California San Diego La Jolla, CA 92093-0378 nholson-at-ucsd.edu http://cryoem.ucsd.edu Cell: (858)220-2183 (858)822-6718 - Office; (858)534-5846 - Fax ______________________________________________________________
The stuff is Ethane, 1,1-Difluoro so it is Difluoroethane rather than yours which is tetrafluoroethane. The label does not list the "bitterant" but warns about its presence. The product code of the propellant is 152a. The other propellant is 143a, which is probably what you have.
Since the can does not state the propellant type, I suppose if it says "Contains a bitterant to help discourage inhalant abuse" it uses 152a. Without the warning, it is probably 143a.
In any event, Falcon says that the bitterant model with 152a is for computers, electronics, optics, etc.--exactly what we need. The alternate 143a is for flamability reduction applications.
I'll get some cans from Office Depot and try both versions. I usually get the dusters from a camera store since they use the dusters to clean lenses and front coated mirrors. So that ought to be safe stuff to use for EM and LM.
So, I guess that either type is not useless. Oh, Falcon makes a point of the cans NOT being canned air.
gary g.
At 09:45 AM 9/4/2007, you wrote:
} Hello Gary, } } Because we have some kind of contract with Office Depot, we get our } canned dusters there. The label says it contains 100% } tetrafluoroethane. The can doesn't indicate that it contains any other } ingredients nor are there any warnings/notifications about anti-huffing } additives, etc. } } On your Dust-off cans, does it list an anti-huffing agent? Or did you } just notice that it started behaving differently or something? I looked } on their website, just to see how they are justifying this or whatever, } and they make no mention of this. This makes me paranoid that these } chemicals may be in my dusters, too, and I just haven't noticed. } } Thanks, } } Andy Bowling } } } -----Original Message----- } From: gary-at-gaugler.com [mailto:gary-at-gaugler.com] } Sent: Thursday, August 30, 2007 6:26 PM } To: Bowling, Andrew } Subject: [Microscopy] Useless canned air } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
Gary, The difluoroethane (152a) is flammable. I haven't seen the stuff, but it seems (from other conversations I've had) that it is prevalent in CA. The tetrafluoroethane (134a, not 143a)is non-flammable and available from Chemtronics, SPI and others. I've also seen a mix out there of some HFC mixed with ether (methyl ether, I believe) which is also flammable. Personally, I stick with the 134a for leak checking with a Varian Smart Gauge or a refrigerant sniffer, for gross leaks, and in my shop I have a Craftsman 20 gal. air compressor and a filter specifically designed to remove oil (about $100 from Grainger and others) that gives me plenty of, what to this point has seemed, very clean air. I have sometimes gotten oily water from the hose that runs directly off the tank, but never from anything that goes through the filter. My SEMs and vacuum evaporator use unfiltered air, but I have several hoses around the shop that use the filtered air.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: Tuesday, September 04, 2007 5:06 PM To: kenconverse-at-qualityimages.biz
The stuff is Ethane, 1,1-Difluoro so it is Difluoroethane rather than yours which is tetrafluoroethane. The label does not list the "bitterant" but warns about its presence. The product code of the propellant is 152a. The other propellant is 143a, which is probably what you have.
Since the can does not state the propellant type, I suppose if it says "Contains a bitterant to help discourage inhalant abuse" it uses 152a. Without the warning, it is probably 143a.
In any event, Falcon says that the bitterant model with 152a is for computers, electronics, optics, etc.--exactly what we need. The alternate 143a is for flamability reduction applications.
I'll get some cans from Office Depot and try both versions. I usually get the dusters from a camera store since they use the dusters to clean lenses and front coated mirrors. So that ought to be safe stuff to use for EM and LM.
So, I guess that either type is not useless. Oh, Falcon makes a point of the cans NOT being canned air.
gary g.
At 09:45 AM 9/4/2007, you wrote:
} Hello Gary, } } Because we have some kind of contract with Office Depot, we get our } canned dusters there. The label says it contains 100% } tetrafluoroethane. The can doesn't indicate that it contains any other } ingredients nor are there any warnings/notifications about anti-huffing } additives, etc. } } On your Dust-off cans, does it list an anti-huffing agent? Or did you } just notice that it started behaving differently or something? I } looked on their website, just to see how they are justifying this or } whatever, and they make no mention of this. This makes me paranoid } that these chemicals may be in my dusters, too, and I just haven't } noticed. } } Thanks, } } Andy Bowling } } } -----Original Message----- } From: gary-at-gaugler.com [mailto:gary-at-gaugler.com] } Sent: Thursday, August 30, 2007 6:26 PM } To: Bowling, Andrew } Subject: [Microscopy] Useless canned air } } } } } ----------------------------------------------------------------------- } - } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
You can get small portable oxygen sensors which are useful also. However, they have a limited battery life, and they need to be recalibrated preferably annually at minimum, just like the permanently installed oxygen sensors, as outlined by Kelly.
cheers, Rsemary
Dr Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 61 (0)2-6246 5475 GPO Box 1600 fax. 61 (0)2-6246 5334 Canberra, ACT 2601 Australia
} From: KellyARamos-at-Eaton.com } Reply-To: KellyARamos-at-Eaton.com } Date: Tue, 4 Sep 2007 15:59:53 -0500 } To: rosemary.white-at-csiro.au } Subject: [Microscopy] re: oxygen deprivation (Installation of an OXYGEN } Sensor) } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Howdy, All. } } We are a commercial testing laboratory and We use nitrogen lines (have a } hook up coming from the plant supply) to Run some of our equipment and } also to blow off samples in our metallography area. } This works out a lot better for us due to the fact that the plant air } lines sometimes get water in them. We are talking about getting quite a } substantial stream of water out of an air line, instead of just air. } Not good! } } The thing we have done for safety, is to install Oxygen sensors in the } vicinity of these nitrogen supply lines. And these sensors are connected } to Very LOUD Alarm and large red flashing lights outside of the rooms } where nitrogen supplies are present. The alarms are set to go off if it } registers oxygen less than around 19%or20%. The sensors are serviced on } a regular basis. } } } Peace, } Kelly A. Ramos } } } -----Original Message----- } X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com] } Sent: Tuesday, September 04, 2007 4:30 PM } To: Ramos, Kelly A } Subject: [Microscopy] RE: re: oxygen deprivation } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } Back when we still had a darkroom with rotary doors, the safety people } asked us to put in extra heavy duty hoses for the nitrogen guns that we } used to blow off dust. We thought they were going overboard at first } when they chose 12,000 psi rated brake hoses, but this was judged much } cheaper than oxygen sensors, and when we thought about the scenario they } were designed to prevent, we thought it was OK. What is the scenario? } Imagine a hose fails in the middle of the night or weekend. The room } fills with nitrogen, displacing all of the air. A person entering the } darkroom through the rotary doors would be breathing 100% nitrogen, and } would pass out after taking one breath. Nobody on the outside would see } them. Anyone entering the darkroom would suffer the same fate. These } would become fatalities. Basically, the consequences of a nitrogen leak } were deemed so severe that 12,000 psi rated brake hose nitrogen lines } seemed reasonable when used in darkroom with rotary doors. } } John Mardinly } Intel } } This is not an opinion of Intel Corporation. } } -----Original Message----- } X-from: nholson-at-ucsd.edu [mailto:nholson-at-ucsd.edu] } Sent: Tuesday, September 04, 2007 12:42 PM } To: Mardinly, John } Subject: [Microscopy] re: oxygen deprivation } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } } -- } Along this same line of thought, does anyone know of any in-room } sensor that can be purchased that emits an alarm if oxygen levels get } low. I am thinking of something that works like either a smoke alarm } or a carbon monoxide detector. We need to use ethane gas for our } cryo procedures and the safety people are causing an uproar about } that. These people tend to make a "one size fits all" type of } regulation that often has little relevance to specific situations. } If anyone has any specific product I would appreciate the info. } } Norm Olson } } } ______________________________________________________________ } Norm Olson } Cryoelectron Microscopy Facilities Manager } 1510 Bonner Hall } Department of Chemistry & Biochemistry, MC-0378 } University of California San Diego } La Jolla, CA 92093-0378 } nholson-at-ucsd.edu } http://cryoem.ucsd.edu } Cell: (858)220-2183 } (858)822-6718 - Office; (858)534-5846 - Fax } ______________________________________________________________ } } ==============================Original } Headers============================== } 5, 23 -- From nholson-at-ucsd.edu Tue Sep 4 14:41:50 2007 } 5, 23 -- Received: from outbound2.ucsd.edu (outbound2.ucsd.edu } [132.239.1.206]) } 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l84JfnIH013700 } 5, 23 -- for {microscopy-at-microscopy.com} ; 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} 13, 35 -- Tue, 4 Sep 2007 13:23:53 -0700 } 13, 35 -- Received: from scsmsx415.amr.corp.intel.com ([10.3.90.34]) by } scsmsx411.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); } 13, 35 -- Tue, 4 Sep 2007 13:23:52 -0700 } 13, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 13, 35 -- Content-class: urn:content-classes:message } 13, 35 -- MIME-Version: 1.0 } 13, 35 -- Content-Type: text/plain; } 13, 35 -- charset="us-ascii" } 13, 35 -- Subject: RE: [Microscopy] re: oxygen deprivation } 13, 35 -- Date: Tue, 4 Sep 2007 13:23:51 -0700 } 13, 35 -- Message-ID: } {F3CB8931ABF8294DB889E977150CAD680108A7D4-at-scsmsx415.amr.corp.intel.com} } 13, 35 -- In-Reply-To: {200709041941.l84JfvEq013809-at-ns.microscopy.com} } 13, 35 -- X-MS-Has-Attach: } 13, 35 -- X-MS-TNEF-Correlator: } 13, 35 -- Thread-Topic: [Microscopy] re: oxygen deprivation } 13, 35 -- Thread-Index: AcfvK6k5dK873fp0TFSABDdM4wfMvgABCbQQ } 13, 35 -- References: {200709041941.l84JfvEq013809-at-ns.microscopy.com} } 13, 35 -- From: "Mardinly, John" {john.mardinly-at-intel.com} } 13, 35 -- To: {nholson-at-ucsd.edu} } 13, 35 -- Cc: {Microscopy-at-msa.microscopy.com} } 13, 35 -- X-OriginalArrivalTime: 04 Sep 2007 20:23:52.0454 (UTC) } FILETIME=[8275C260:01C7EF31] } 13, 35 -- Content-Transfer-Encoding: 8bit } 13, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id l84KNrdW026126 } ==============================End of - } Headers============================== } } } ==============================Original Headers============================== } 25, 27 -- From KellyARamos-at-Eaton.com Tue Sep 4 15:54:09 2007 } 25, 27 -- Received: from tccbw2.etn.com (mail.eaton.com [192.104.67.6]) } 25, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l84Ks99s006035 } 25, 27 -- for {Microscopy-at-msa.microscopy.com} ; 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I have a client who needs to remove wax from the surfaces of leaves so that she can count stomates in the FESEM. A first try at soaking the leaves in 2 changes of xylene, 10 minutes each, didn't do much at all. the leaves are highly papilose (is that a word? Have lots and lots of pappillae) and have lots of wax. Peels are not an option. Any suggestions appreciated!
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 5, 19 -- From tina-at-pbrc.hawaii.edu Tue Sep 4 19:44:12 2007 5, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l850iBmR025045 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Sep 2007 19:44:11 -0500 5, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id l850i8Pe009648 5, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Sep 2007 14:44:08 -1000 (HST) 5, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id l850i7cM009644 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Sep 2007 14:44:07 -1000 (HST) 5, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 5, 19 -- Date: Tue, 4 Sep 2007 14:44:06 -1000 (HST) 5, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 19 -- X-Sender: tina-at-halia 5, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 5, 19 -- Subject: Dewaxing leaf surface for SEM 5, 19 -- Message-ID: {Pine.GSO.4.21.0709041441170.9628-100000-at-halia} 5, 19 -- MIME-Version: 1.0 5, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
Hi Tina, You could try sluicing the surface with chloroform, that sometimes works well cheers Sally
Sally Stowe ANU EMU Canberra
On Wed, September 5, 2007 10:44 am, tina-at-pbrc.hawaii.edu wrote: }
} } } ------------------------------------------------------------------------- } --- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------- } -- } } } Hi, All- } } } I have a client who needs to remove wax from the surfaces of leaves so } that she can count stomates in the FESEM. A first try at soaking the leaves } in 2 changes of xylene, 10 minutes each, didn't do much at all. the leaves } are highly papilose (is that a word? Have lots and lots of pappillae) and } have lots of wax. Peels are not an option. Any suggestions appreciated! } } Aloha, Tina } } } ************************************************************************* } *** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu } * } * Biological Electron Microscope Facility * (808) 956-6251 } * } * University of Hawaii at Manoa * } http://www.pbrc.hawaii.edu/bemf* } ************************************************************************** } ** } } } } ==============================Original } Headers============================== } 5, 19 -- From tina-at-pbrc.hawaii.edu Tue Sep 4 19:44:12 2007 } 5, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu } [128.171.22.7]) } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l850iBmR025045 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Sep } 2007 19:44:11 -0500 } 5, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) } 5, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id } l850i8Pe009648 5, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA } bits=256 verify=NO) 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 } Sep 2007 14:44:08 -1000 (HST) } 5, 19 -- Received: from localhost by halia.pbrc.hawaii.edu } (8.12.11/8.12.11/Submit) with ESMTP id l850i7cM009644 } 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Sep 2007 14:44:07 } -1000 (HST) } 5, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned } process doing -bs 5, 19 -- Date: Tue, 4 Sep 2007 14:44:06 -1000 (HST) } 5, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} } 5, 19 -- X-Sender: tina-at-halia } 5, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} } 5, 19 -- Subject: Dewaxing leaf surface for SEM } 5, 19 -- Message-ID: {Pine.GSO.4.21.0709041441170.9628-100000-at-halia} } 5, 19 -- MIME-Version: 1.0 } 5, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 7, 27 -- From sally.stowe-at-anu.edu.au Tue Sep 4 20:19:50 2007 7, 27 -- Received: from mail.rsbs.anu.edu.au (mail.rsbs.anu.edu.au [150.203.72.70]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l851JnN0004902 7, 27 -- for {microscopy-at-msa.microscopy.com} ; Tue, 4 Sep 2007 20:19:50 -0500 7, 27 -- Received: from mail.rsbs.anu.edu.au (mail.rsbs.anu.edu.au [127.0.0.1]) 7, 27 -- by mail.rsbs.anu.edu.au (Postfix) with ESMTP 7, 27 -- id 04FDFF44059; Wed, 5 Sep 2007 11:19:45 +1000 (EST) 7, 27 -- Received: from 150.203.36.128 7, 27 -- (SquirrelMail authenticated user u8411377) 7, 27 -- by mail.rsbs.anu.edu.au with HTTP; 7, 27 -- Wed, 5 Sep 2007 11:19:45 +1000 (EST) 7, 27 -- Message-ID: {4861.150.203.36.128.1188955185.squirrel-at-mail.rsbs.anu.edu.au} 7, 27 -- In-Reply-To: {200709050044.l850iMEv025194-at-ns.microscopy.com} 7, 27 -- References: {200709050044.l850iMEv025194-at-ns.microscopy.com} 7, 27 -- Date: Wed, 5 Sep 2007 11:19:45 +1000 (EST) 7, 27 -- Subject: Re: [Microscopy] Dewaxing leaf surface for SEM 7, 27 -- From: Sally.Stowe-at-anu.edu.au 7, 27 -- To: tina-at-pbrc.hawaii.edu 7, 27 -- Cc: microscopy-at-msa.microscopy.com 7, 27 -- Reply-To: sally.stowe-at-anu.edu.au 7, 27 -- User-Agent: SquirrelMail/1.5.1 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain;charset=iso-8859-1 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-RSBS-MailScanner-Information: Please contact the ISP for more information 7, 27 -- X-RSBS-MailScanner: Found to be clean 7, 27 -- X-MailScanner-From: sally.stowe-at-anu.edu.au ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both plarson-at-ou.edu, gstrout-at-ou.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] SDD detetectors - are they there yet for high res TEM X-ray applications?
Question: We are in the process of examining EDX systems for our newly acquired JEOL 2010F TEM. The microscope will be used mostly for materials/nano work where we anticipate high mags, small analysis spots, and correspondingly low count rates. Additionally, we expect some light element work on occasion. Up to now we have looked at "traditional" systems from the major manufacturers fitted with Ln2 cooled SiLi detectors. We are however, aware of the increasing presence of Peltier cooled Silicon Drift Detectors (SDD) supplied with EDX systems primarily from the SEM and XRF markets, although most of the comparable TEMs we have seen are equipped with SiLi detectors. It is our understanding that SDDs have recently improved in performance with advertised resolutions comparable to, or better than, SiLi detectors and have generally been known for having high throughputs. It would seem that having an LN2 dewar hanging on the microscope also introduces noise and vibration into the column. On the other hand, we have heard that SDDs have an efficiency fall off above 12kev and that they can be expensive to repair. What are the consequences, if any, of this fall off? Our naive assumption is that we would not see the K alpha peaks for the higher Z elements but the lower energy L and M peaks for these elements would still presumably show up. Further, should we have any concerns about possible radiation damage from high energy back scattered electrons striking the detector in an SDD? In short, we would appreciate any comments or suggestions from the listserv in regards to these issues or any other issues we may have missed regarding the use of an SDD detector on a high resolution field emission TEM. Thanks in advance, gstrout-at-ou.edu plarson-at-ou.edu -- -- ================================================================== Greg Strout Electron Microscopist, University of Oklahoma Phone: (405) 325-4391 e-mail: gstrout-at-ou.edu Opinions expressed herein are mine and not necessarily those of the University of Oklahoma ==================================================================
================================================================== Preston Larson Research Scientist Samuel Roberts Noble Electron Microscopy Laboratory 770 Van Vleet Oval Phone: (405) 325-4391 ==================================================================
SDD has certainly had major advances. There are 10^2mm, 20^2mm and 40^2mm detectors. There might be some 30 units. So far, my results are from SEM. But there is strong correlation with STEM. I am using EDAX 40^2mm SDD...Apollo 40.
The latest generation of SDD by EDAX is totally awesome. They replaced their Cryospec Clemenko cryocooler system with this new SDD. They have 10^2mm or 40^2 mm. I am using the Apollo 40. I can dump a huge amount of counts at it and still keep DT low. The trick is to keep DT and resolution in an area/zone where peaks are discernable. This means that if you want high cps, you will need shorter time constants. That said, resolution will degrade. If your Z list is wide, that is not a problem. If close Z values, one must suffer longer collection times and poor resolution between Z.
I cannot imagine a SDD that has the same resolution at every time constant. You, as the operator, has to make a tradeoff between clock time and accuracy.
SDD is the future, IMO. Si(LI) is history.
No financial interest in EDAX other than to hope that they remain viable.
gary g.
At 05:53 PM 9/4/2007, you wrote:
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==============================Original Headers============================== 17, 21 -- From gary-at-gaugler.com Tue Sep 4 22:50:24 2007 17, 21 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 17, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l853oNbo031631 17, 21 -- for {microscopy-at-microscopy.com} ; Tue, 4 Sep 2007 22:50:23 -0500 17, 21 -- Message-Id: {200709050350.l853oNbo031631-at-ns.microscopy.com} 17, 21 -- Received: (qmail 20858 invoked from network); 4 Sep 2007 20:50:23 -0700 17, 21 -- Received: by simscan 1.1.0 ppid: 20855, pid: 20856, t: 0.1450s 17, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 17, 21 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 17, 21 -- by qsmtp4 with SMTP; 4 Sep 2007 20:50:23 -0700 17, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 17, 21 -- Date: Tue, 04 Sep 2007 20:50:31 -0800 17, 21 -- To: plarson-at-ou.edu 17, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 17, 21 -- Subject: Re: [Microscopy] viaWWW: SDD detetectors - are they there yet 17, 21 -- for high res TEM 17, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 17, 21 -- In-Reply-To: {200709050153.l851r917019980-at-ns.microscopy.com} 17, 21 -- References: {200709050153.l851r917019980-at-ns.microscopy.com} 17, 21 -- Mime-Version: 1.0 17, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-3460431A ==============================End of - Headers==============================
I am posting the following on behalf of a colleague. Please address replies to Dr Peta Clode (peta.clode-at-uwa.edu.au).
Thanks,
Martin.
Question:
We are using TEM to look at cells that are grown on thermonox coverslips - however, when we try to section them for TEM the thermonox pulls away from the epoxy and we are left with our cells sitting along the edge of the section, which is largely unstable in the TEM. As such we have to remove the thermonox and re-embed them before sectioning, which rather defeats the purpose of using thermonox.
Does anyone have any tips for preparing cells on thermonox for TEM?
--
*****************************************
Dr. Martin Saunders, Deputy Director and Senior Lecturer, Centre for Microscopy, Characterisation and Analysis, M010, The University of Western Australia, Crawley, Western Australia 6009, Australia.
Meantime we are using Millipore filters to circumvent this problem. You have to avoid certain solvents, but infiltration with EtOH diluted epoxy resin is OK as long as you have extra 'pure resin' steps to remove trace EtOH before embedding, and use a flat bottomed resin/solvent resistant LM embedding mold to avoid handling the floppy filter. After polymerisation with a thin layer of resin, the filter can be trimmed to fit into a conventional EM mold for sectioning on edge.
Hope this helps.
Looking forward to other responses from 'listers'.
Best regards,
Alastair
Alastair McKinnon Histology & EM Facility Manager University of Aberdeen, Institute of Medical Science Foresterhill, Aberdeen, AB25 2ZD tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk www.abdn.ac.uk/ims/h-em -----Original Message----- X-from: martin-at-cmm.uwa.edu.au [mailto:martin-at-cmm.uwa.edu.au] Sent: 05 September 2007 06:26 To: Mckinnon, Alastair D.
Dear all,
I am posting the following on behalf of a colleague. Please address replies to Dr Peta Clode (peta.clode-at-uwa.edu.au).
Thanks,
Martin.
Question:
We are using TEM to look at cells that are grown on thermonox coverslips - however, when we try to section them for TEM the thermonox pulls away from the epoxy and we are left with our cells sitting along the edge of the section, which is largely unstable in the TEM. As such we have to remove the thermonox and re-embed them before sectioning, which rather defeats the purpose of using thermonox.
Does anyone have any tips for preparing cells on thermonox for TEM?
--
*****************************************
Dr. Martin Saunders, Deputy Director and Senior Lecturer, Centre for Microscopy, Characterisation and Analysis, M010, The University of Western Australia, Crawley, Western Australia 6009, Australia.
This Question was submitted to Ask-A-Microscopist by (vina236-at-yahoo.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, September 5, 2007 at 07:57:09 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both vina236-at-yahoo.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
When at a previous lab, I processed one batch of leaves for SEM using standard ethanol dehydration with CPD and got wonderful shots of waxy cuticle with stomata peeking through. A second batch was dehydrated in an acetone series, followed CPD in 100% ethanol and the surface was beautifully cleaned of wax.
For what it's worth. It worked for me.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: tina-at-pbrc.hawaii.edu [mailto:tina-at-pbrc.hawaii.edu] Sent: Tuesday, September 04, 2007 7:45 PM To: Tindall, Randy D.
Hi, All-
I have a client who needs to remove wax from the surfaces of leaves so that she can count stomates in the FESEM. A first try at soaking the leaves in 2 changes of xylene, 10 minutes each, didn't do much at all. the leaves are highly papilose (is that a word? Have lots and lots of pappillae) and have lots of wax. Peels are not an option. Any suggestions appreciated!
Aloha, Tina
************************************************************************ **** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ************************************************************************ ****
==============================Original Headers============================== 5, 19 -- From tina-at-pbrc.hawaii.edu Tue Sep 4 19:44:12 2007 5, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l850iBmR025045 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Sep 2007 19:44:11 -0500 5, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id l850i8Pe009648 5, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Sep 2007 14:44:08 -1000 (HST) 5, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id l850i7cM009644 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Sep 2007 14:44:07 -1000 (HST) 5, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 5, 19 -- Date: Tue, 4 Sep 2007 14:44:06 -1000 (HST) 5, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 19 -- X-Sender: tina-at-halia 5, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 5, 19 -- Subject: Dewaxing leaf surface for SEM 5, 19 -- Message-ID: {Pine.GSO.4.21.0709041441170.9628-100000-at-halia} 5, 19 -- MIME-Version: 1.0 5, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
==============================Original Headers============================== 17, 26 -- From TindallR-at-missouri.edu Wed Sep 5 08:33:48 2007 17, 26 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85DXmCL006767 17, 26 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 08:33:48 -0500 17, 26 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 17, 26 -- Wed, 5 Sep 2007 08:33:47 -0500 17, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 26 -- Content-class: urn:content-classes:message 17, 26 -- MIME-Version: 1.0 17, 26 -- Content-Type: text/plain; 17, 26 -- charset="us-ascii" 17, 26 -- Subject: RE: [Microscopy] Dewaxing leaf surface for SEM 17, 26 -- Date: Wed, 5 Sep 2007 08:33:47 -0500 17, 26 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BD2B-at-UM-XMAIL08.um.umsystem.edu} 17, 26 -- In-Reply-To: {200709050045.l850jPTS026881-at-ns.microscopy.com} 17, 26 -- X-MS-Has-Attach: 17, 26 -- X-MS-TNEF-Correlator: 17, 26 -- Thread-Topic: [Microscopy] Dewaxing leaf surface for SEM 17, 26 -- Thread-Index: AcfvXvSQ3Mb0IplDSn2ELSbJ5ZdpfAAYFpbw 17, 26 -- References: {200709050045.l850jPTS026881-at-ns.microscopy.com} 17, 26 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 17, 26 -- To: {tina-at-pbrc.hawaii.edu} 17, 26 -- Cc: {microscopy-at-microscopy.com} 17, 26 -- X-OriginalArrivalTime: 05 Sep 2007 13:33:47.0865 (UTC) FILETIME=[63652C90:01C7EFC1] 17, 26 -- Content-Transfer-Encoding: 8bit 17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l85DXmCL006767 ==============================End of - Headers==============================
Tina, If the chlorofom doesn't work, you might look into methods for making trichomes and papillae fall off. I think if you freeze the leaf in LN2 you can shear them off. But this is just something I heard of for isolating the trichomes, not for looking at a trichome-free leaf. But the leaf stomata might be easier to see with the hairs gone? I am sorry I can't point you to a citation, but maybe the tentacles of google can oblige?
Tobias } } Hi, All- } } I have a client who needs to remove wax from the surfaces of leaves so } that she can count stomates in the FESEM. A first try at soaking the } leaves in 2 changes of xylene, 10 minutes each, didn't do much at all. the } leaves are highly papilose (is that a word? Have lots and lots of } pappillae) and have lots of wax. Peels are not an option. Any suggestions } appreciated! } } Aloha, Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } **************************************************************************** } } } ==============================Original Headers============================== } 5, 19 -- From tina-at-pbrc.hawaii.edu Tue Sep 4 19:44:12 2007 } 5, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu } [128.171.22.7]) } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l850iBmR025045 } 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Sep 2007 } 19:44:11 -0500 } 5, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) } 5, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP } id l850i8Pe009648 } 5, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA } bits=256 verify=NO) } 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Sep 2007 } 14:44:08 -1000 (HST) } 5, 19 -- Received: from localhost by halia.pbrc.hawaii.edu } (8.12.11/8.12.11/Submit) with ESMTP id l850i7cM009644 } 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Sep 2007 } 14:44:07 -1000 (HST) } 5, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned } process doing -bs } 5, 19 -- Date: Tue, 4 Sep 2007 14:44:06 -1000 (HST) } 5, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} } 5, 19 -- X-Sender: tina-at-halia } 5, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} } 5, 19 -- Subject: Dewaxing leaf surface for SEM } 5, 19 -- Message-ID: {Pine.GSO.4.21.0709041441170.9628-100000-at-halia} } 5, 19 -- MIME-Version: 1.0 } 5, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII } ==============================End of - Headers==============================
What has worked for us recently (with aclar and sapphire discs) is to peel the aclar off post polymerization and add a second layer of the same resin to the block face where the cells are. Seems to work much better than leaving those cells hanging out there. What resin are you using? A second treatment is to be sure you are curing the resin under vacuum. The resin tends to stay very smooth against the aclar (should for thermanox as well) but if not under vacuum there tends to be some roughness which prevent decent sectioning.
Regardless of what resin we have used the first method works pretty well.
Garnet
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-- Garnet Martens
Research Manager BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
phone 604-822-3354
==============================Original Headers============================== 9, 30 -- From gmartens-at-interchange.ubc.ca Wed Sep 5 10:24:11 2007 9, 30 -- Received: from mr7.mail-relay.ubc.ca (mr7.mail-relay.ubc.ca [137.82.45.13]) 9, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85FOB3A000372 9, 30 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 5 Sep 2007 10:24:11 -0500 9, 30 -- X-Ubc-Received: from mr7.mail-relay.ubc.ca (localhost [127.0.0.1]) 9, 30 -- by localhost (Postfix) with SMTP id 70B5A14860 9, 30 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 5 Sep 2007 08:24:10 -0700 (PDT) 9, 30 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 9, 30 -- by mr7.mail-relay.ubc.ca (Postfix) with ESMTP 9, 30 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 5 Sep 2007 08:24:09 -0700 (PDT) 9, 30 -- Received: from [137.82.85.216] (0511.emlab.ubc.ca [137.82.85.216]) 9, 30 -- by smtp.interchange.ubc.ca 9, 30 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 9, 30 -- with ESMTPA id {0JNW00GMRIS8CK-at-smtp.interchange.ubc.ca} for 9, 30 -- Microscopy-at-MSA.Microscopy.Com; Wed, 05 Sep 2007 08:24:09 -0700 (PDT) 9, 30 -- Date: Wed, 05 Sep 2007 08:24:47 -0700 9, 30 -- From: Garnet Martens {gmartens-at-interchange.ubc.ca} 9, 30 -- Subject: Re: [Microscopy] TEM - cells on thermonox 9, 30 -- In-reply-to: {200709050524.l855Ookr018137-at-ns.microscopy.com} 9, 30 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 9, 30 -- Cc: peta.clode-at-uwa.edu.au 9, 30 -- Message-id: {a06240804c30479578983-at-[137.82.85.216]} 9, 30 -- MIME-version: 1.0 9, 30 -- Content-type: text/plain; format=flowed; charset=us-ascii 9, 30 -- References: {200709050524.l855Ookr018137-at-ns.microscopy.com} 9, 30 -- X-UBC-Scanned: Sophos PureMessage 5.3.3.310218, Antispam-Engine: 0.0.4.314406, Antispam-Data: 2007.9.5.74728 9, 30 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 9, 30 -- X-PerlMx-Spam: Probability=7%, Report=__C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0 9, 30 -- X-Spam-Level: 9, 30 -- X-Spam-Flag: No ==============================End of - Headers==============================
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==============================Original Headers============================== 12, 23 -- From nancy-at-savvyhire.com Wed Sep 5 10:36:39 2007 12, 23 -- Received: from ms-smtp-05.socal.rr.com (ms-smtp-05.socal.rr.com [66.75.162.137]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85FadOD012486 12, 23 -- for {Microscopy-at-Microscopy.Com} ; Wed, 5 Sep 2007 10:36:39 -0500 12, 23 -- Received: from nks (cpe-66-91-245-208.san.res.rr.com [66.91.245.208]) 12, 23 -- by ms-smtp-05.socal.rr.com (8.13.6/8.13.6) with ESMTP id l85FaPJH008950 12, 23 -- for {Microscopy-at-Microscopy.Com} ; Wed, 5 Sep 2007 08:36:38 -0700 (PDT) 12, 23 -- From: "Nancy Stewart" {nancy-at-savvyhire.com} 12, 23 -- To: {Microscopy-at-Microscopy.Com} 12, 23 -- Subject: System Specialist job opening 12, 23 -- Date: Wed, 5 Sep 2007 08:36:12 -0700 12, 23 -- Organization: Savvy Hire 12, 23 -- Message-ID: {001201c7efd2$823265a0$6401a8c0-at-nks} 12, 23 -- MIME-Version: 1.0 12, 23 -- Content-Type: text/plain; 12, 23 -- charset="us-ascii" 12, 23 -- Content-Transfer-Encoding: 7bit 12, 23 -- X-Priority: 3 (Normal) 12, 23 -- X-MSMail-Priority: Normal 12, 23 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 12, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 12, 23 -- Importance: Normal 12, 23 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
One method of doing this is to use the "pop-off" technique. Note that this also removes the cover slip, so if you need a cross-sectional view of the cells including the attachment area between the cells and coverslip, this method will not work for you. However, if your main problem is that the cells end up on the edge of the section and are therefore unstable for viewing, the pop-off method works just fine.
Grow your cells normally on the coverslips and process them through your final pure resin infiltration stages. Then fill your embedding molds with resin and leave a small meniscus at the top. Put your coverslips, cell side down, on top of the meniscus on the embedding capsules, and polymerize. When the blocks are still somewhat incompletely polymerized, remove them from the oven and bring them to room temperature. Put some liquid nitrogen in a small, insulated container and either submerge the coverslip end of the block into the liquid nitrogen or hold it in the vapor phase. You may hear some crackling sounds, which is usually good.
After a few seconds of freezing, remove the block from the LN2 and try to peel off the coverslip. If it snaps right off, great! If not, try again. It may take a few tries, but when the coverslip comes off, the cells will remain in the resin and may be sectioned as a monolayer. Look at the block under a dissecting scope to identify areas with plentiful cells, trim away the excess (you may want to save the trimmed chunks as backups), and carefully set up your approach on the microtome. There is only a monolayer of cells, so you will need to start collecting sections as soon as they are big enough to pick up on grids. You will be sectioning from the side of the cell which attached to the coverslip, up to the top of the cell.
A couple cautions---sometimes (rarely) a block will shatter in the liquid nitrogen. If so, it will usually hang together and you can find pieces with cells and remount them on another block. Also, trimming the block face and taking the initial sections need to be done carefully so you don't accidentally lose all your cells. Making extra blocks is good insurance, as always. Finally, Thermonox coverslips generally are much easier to use than glass, simply because glass likes to shatter when it is removed from the cold block face, leaving lots of little pieces that must be removed individually.
That said, this is a very reliable and fairly easy technique.
Good luck!
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 10, 24 -- From TindallR-at-missouri.edu Wed Sep 5 11:00:33 2007 10, 24 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85G0Xmw015798 10, 24 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 11:00:33 -0500 10, 24 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 10, 24 -- Wed, 5 Sep 2007 11:00:32 -0500 10, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 24 -- Content-class: urn:content-classes:message 10, 24 -- MIME-Version: 1.0 10, 24 -- Content-Type: text/plain; 10, 24 -- charset="us-ascii" 10, 24 -- Subject: TEM: Cells on Thermonox cover slips 10, 24 -- Date: Wed, 5 Sep 2007 11:00:32 -0500 10, 24 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BD2F-at-UM-XMAIL08.um.umsystem.edu} 10, 24 -- X-MS-Has-Attach: 10, 24 -- X-MS-TNEF-Correlator: 10, 24 -- Thread-Topic: TEM: Cells on Thermonox cover slips 10, 24 -- Thread-Index: Acfv1ePsLt0oXQ9yQp2lm7zR0qSJLQ== 10, 24 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 10, 24 -- To: {peta.clode-at-uwa.edu.au} 10, 24 -- Cc: {microscopy-at-microscopy.com} 10, 24 -- X-OriginalArrivalTime: 05 Sep 2007 16:00:32.0722 (UTC) FILETIME=[E37FBB20:01C7EFD5] 10, 24 -- Content-Transfer-Encoding: 8bit 10, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l85G0Xmw015798 ==============================End of - Headers==============================
Gary; I do not agree that "Si(LI) is history." TEM's are hampered by low count rates, especially if one desires to do more than simple spectroscopy and would like to do compositional line profiles and spectral imaging. There are at least two vendors I know of that offer 50 square mm detectors, and they offer tremendous advantages in count rates. Also, Si detectors are much thicker that drift detectors, which transmit (and do not detect) most X-ray's over 12 KeV. Elements with L series in the mid 10KeV range are difficult to deal with due to overlaps. Working with the K series in the 15-25KeV range offers significant advantage for those elements. The only barrier for some TEM's is these detectors may not fit, and for our 2010F, the anticontamination device (inside the pole piece) had to be modified. The parts were expensive, but JEOL had the parts as a factory configuration and the service engineers were able to pull the gun, split the column, put everything back together, and start pumping in 7 hours, so don't be afraid of doing the modification. For us it was well worth it.
John Mardinly Intel
This is not an opinion of Intel Corporation.
-----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: Tuesday, September 04, 2007 8:51 PM To: Mardinly, John
SDD has certainly had major advances. There are 10^2mm, 20^2mm and 40^2mm detectors. There might be some 30 units. So far, my results are from SEM. But there is strong correlation with STEM. I am using EDAX 40^2mm SDD...Apollo 40.
The latest generation of SDD by EDAX is totally awesome. They replaced their Cryospec Clemenko cryocooler system with this new SDD. They have 10^2mm or 40^2 mm. I am using the Apollo 40. I can dump a huge amount of counts at it and still keep DT low. The trick is to keep DT and resolution in an area/zone where peaks are discernable. This means that if you want high cps, you will need shorter time constants. That said, resolution will degrade. If your Z list is wide, that is not a problem. If close Z values, one must suffer longer collection times and poor resolution between Z.
I cannot imagine a SDD that has the same resolution at every time constant. You, as the operator, has to make a tradeoff between clock time and accuracy.
SDD is the future, IMO. Si(LI) is history.
No financial interest in EDAX other than to hope that they remain viable.
gary g.
At 05:53 PM 9/4/2007, you wrote:
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==============================Original Headers============================== 17, 21 -- From gary-at-gaugler.com Tue Sep 4 22:50:24 2007 17, 21 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 17, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l853oNbo031631 17, 21 -- for {microscopy-at-microscopy.com} ; Tue, 4 Sep 2007 22:50:23 -0500 17, 21 -- Message-Id: {200709050350.l853oNbo031631-at-ns.microscopy.com} 17, 21 -- Received: (qmail 20858 invoked from network); 4 Sep 2007 20:50:23 -0700 17, 21 -- Received: by simscan 1.1.0 ppid: 20855, pid: 20856, t: 0.1450s 17, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 17, 21 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 17, 21 -- by qsmtp4 with SMTP; 4 Sep 2007 20:50:23 -0700 17, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 17, 21 -- Date: Tue, 04 Sep 2007 20:50:31 -0800 17, 21 -- To: plarson-at-ou.edu 17, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 17, 21 -- Subject: Re: [Microscopy] viaWWW: SDD detetectors - are they there yet 17, 21 -- for high res TEM 17, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 17, 21 -- In-Reply-To: {200709050153.l851r917019980-at-ns.microscopy.com} 17, 21 -- References: {200709050153.l851r917019980-at-ns.microscopy.com} 17, 21 -- Mime-Version: 1.0 17, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-3460431A ==============================End of - Headers==============================
==============================Original Headers============================== 25, 35 -- From john.mardinly-at-intel.com Wed Sep 5 11:09:16 2007 25, 35 -- Received: from mga03.intel.com (mga03.intel.com [143.182.124.21]) 25, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85G9Fsn027857 25, 35 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 5 Sep 2007 11:09:15 -0500 25, 35 -- Received: from azsmga001.ch.intel.com ([10.2.17.19]) 25, 35 -- by azsmga101.ch.intel.com with ESMTP; 05 Sep 2007 09:09:14 -0700 25, 35 -- X-ExtLoop1: 1 25, 35 -- X-IronPort-AV: E=Sophos;i="4.20,211,1186383600"; 25, 35 -- d="scan'208";a="272613049" 25, 35 -- Received: from fmsmsx334.amr.corp.intel.com ([132.233.42.1]) 25, 35 -- by azsmga001.ch.intel.com with ESMTP; 05 Sep 2007 09:09:12 -0700 25, 35 -- Received: from scsmsx412.amr.corp.intel.com ([10.3.90.31]) by fmsmsx334.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 25, 35 -- Wed, 5 Sep 2007 09:09:03 -0700 25, 35 -- Received: from scsmsx415.amr.corp.intel.com ([10.3.90.34]) by scsmsx412.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 25, 35 -- Wed, 5 Sep 2007 09:09:03 -0700 25, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 25, 35 -- Content-class: urn:content-classes:message 25, 35 -- MIME-Version: 1.0 25, 35 -- Content-Type: text/plain; 25, 35 -- charset="us-ascii" 25, 35 -- Subject: RE: [Microscopy] Re: viaWWW: SDD detetectors - are they there yet 25, 35 -- Date: Wed, 5 Sep 2007 09:09:03 -0700 25, 35 -- Message-ID: {F3CB8931ABF8294DB889E977150CAD68010CBFAA-at-scsmsx415.amr.corp.intel.com} 25, 35 -- In-Reply-To: {200709050350.l853oVmM031742-at-ns.microscopy.com} 25, 35 -- X-MS-Has-Attach: 25, 35 -- X-MS-TNEF-Correlator: 25, 35 -- Thread-Topic: [Microscopy] Re: viaWWW: SDD detetectors - are they there yet 25, 35 -- Thread-Index: Acfvb+kv/PcjWgC5SVqXbi848UWIaQAZSzvA 25, 35 -- References: {200709050350.l853oVmM031742-at-ns.microscopy.com} 25, 35 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 25, 35 -- To: {gary-at-gaugler.com} 25, 35 -- Cc: {Microscopy-at-msa.microscopy.com} 25, 35 -- X-OriginalArrivalTime: 05 Sep 2007 16:09:03.0062 (UTC) FILETIME=[13AF6F60:01C7EFD7] 25, 35 -- Content-Transfer-Encoding: 8bit 25, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l85G9Fsn027857 ==============================End of - Headers==============================
In addition to using acetone or chloroform to de-wax plant surfaces, as others have already mentioned, another solution to use is acidified DMP (2,2-dimethoxypropane).
DMP is actually intended to be used as a dehydrating agent. Right after post-fixation water rinses, you can add DMP and it reacts with water to produce equal parts ethanol and acetone so it dehydrates by chemical conversion of water (endothermic) rather by physical diffusion of water out into a dehydration series. You can go direct into the critical point dryer (using CO2) after 2nd change of acidified DMP (2x, 10-15' each). Usually add 1 drop (0.05 ml) of conc. HCl to 100 ml DMP.
DMP has the side effect, or in this case the benefit, of de-waxing plant surfaces.
The classic paper for DMP dehydration in EM is:
Rapid Chemical Dehydration of Samples for Electron Microscopic Examinations. L.L. Muller and T.J. Jacks. The Journal of Histochemistry and Cytochemistry. Vol. 23, No. 2, pp.107-110, 1975.
Other papers:
2,2-Dimethoxypropane, a rapid dehydrating agent for scanning electron microscopy. W.S.Johnson, G.R. Hooper, B.F. Holdaway, and H.P. Rasumssen. Micron, 1976, Vol. 7:305-306.
Rapid Chemical Dehydration of Biologic Samples for Scanning Electron Microscopy using 2,2-Dimethoxypropane. Morton D. Maser and John J. Trimble, III. The Journal of Histochemistry and Cytochemistry. Vol. 25, No. 4, pp. 247-251. 1977.
Optimization and Investigation of the Use of 2,2-Dimethoxypropane as a Dehydration Agent for Plant Tissues in Transmission Electron Microscopy. J.R. Thorpe and Diana M.R. Harvey. Journal of Ultrastruture Research, 68, 186-194 (1979).
Hope this helps, or gives you another option to try for de-waxing plant surfaces or for dehydration in general.
Gib -- Gilbert (Gib) Ahlstrand, Electron Microscopist, Imaging Center, University of Minnesota 123 Snyder Hall St. Paul, MN 55108 http://www.cbs.umn.edu/ic
-----------------------------------------------
tina-at-pbrc.hawaii.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, All- } } I have a client who needs to remove wax from the surfaces of leaves so } that she can count stomates in the FESEM. A first try at soaking the } leaves in 2 changes of xylene, 10 minutes each, didn't do much at all. the } leaves are highly papilose (is that a word? Have lots and lots of } pappillae) and have lots of wax. Peels are not an option. Any suggestions } appreciated! } } Aloha, Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* }
To support the edges of your sections, you can mount them on formvar- coated grids. That will keep them from curling and melting under the electron beam.
Good luck!
Dotty Sorenson
On Sep 5, 2007, at 1:24 AM, martin-at-cmm.uwa.edu.au wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Dear all, } } I am posting the following on behalf of a colleague. Please address } replies to Dr Peta Clode (peta.clode-at-uwa.edu.au). } } Thanks, } } Martin. } } Question: } } We are using TEM to look at cells that are grown on thermonox } coverslips - however, when we try to section them for TEM the } thermonox pulls away from the epoxy and we are left with our cells } sitting along the edge of the section, which is largely unstable in } the TEM. As such we have to remove the thermonox and re-embed them } before sectioning, which rather defeats the purpose of using } thermonox. } } Does anyone have any tips for preparing cells on thermonox for TEM? } } -- } } ***************************************** } } Dr. Martin Saunders, } Deputy Director and Senior Lecturer, } Centre for Microscopy, Characterisation and Analysis, } M010, } The University of Western Australia, } Crawley, } Western Australia 6009, } Australia. } } Phone: +61 8 6488 8092 } Fax: +61 8 6488 1087 } E-mail: Martin.Saunders-at-uwa.edu.au } CRICOS Provider No. 00126G } } ***************************************** } } } ==============================Original } Headers============================== } 13, 31 -- From martin-at-cmm.uwa.edu.au Wed Sep 5 00:21:12 2007 } 13, 31 -- Received: from asclepius2.uwa.edu.au } (asclepius3.uwa.edu.au [130.95.128.60]) } 13, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l855LBZ7012510 } 13, 31 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 5 Sep 2007 } 00:21:12 -0500 } 13, 31 -- Received: from kas30pipe.localhost (localhost.localdomain } [127.0.0.1]) } 13, 31 -- by panacea.uwa.edu.au (Postfix) with ESMTP id B11918810E } 13, 31 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 5 Sep 2007 } 13:21:09 +0800 (WST) } 13, 31 -- Received: from panacea (localhost.localdomain [127.0.0.1]) } 13, 31 -- by panacea.prekas (Postfix) with SMTP id B153D882A9 } 13, 31 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 5 Sep 2007 } 13:21:06 +0800 (WST) } 13, 31 -- X-UWA-Client-IP: 130.95.124.130 (UWA) } 13, 31 -- Received: from [130.95.124.130] (awsjoff.cmm.uwa.edu.au } [130.95.124.130]) } 13, 31 -- by panacea.input (Postfix) with ESMTP id 1B51B88254 } 13, 31 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 5 Sep 2007 } 13:21:05 +0800 (WST) } 13, 31 -- Mime-Version: 1.0 } 13, 31 -- Message-Id: {a06240864c303ed41f85c-at-[130.95.124.130]} } 13, 31 -- Date: Wed, 5 Sep 2007 13:21:38 +0800 } 13, 31 -- To: Microscopy-at-MSA.Microscopy.Com } 13, 31 -- From: Martin Saunders {martin-at-cmm.uwa.edu.au} } 13, 31 -- Subject: TEM - cells on thermonox } 13, 31 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } 13, 31 -- X-SpamTest-Envelope-From: martin-at-cmm.uwa.edu.au } 13, 31 -- X-SpamTest-Group-ID: 00000000 } 13, 31 -- X-SpamTest-Info: Profiles 1433 [September 4 2007] } 13, 31 -- X-SpamTest-Info: {HEADERS: header Content-Type found } without required header Content-Transfer-Encoding} } 13, 31 -- X-SpamTest-Info: {MSGID: id-right missed} } 13, 31 -- X-SpamTest-Method: probable } 13, 31 -- X-SpamTest-Rate: 60 } 13, 31 -- X-SpamTest-Status: UWA Probable } 13, 31 -- X-SpamTest-Status-Extended: probable_spam } 13, 31 -- X-SpamTest-Version: SMTP-Filter Version 3.0.0 [0278], } KAS30/Release } ==============================End of - } Headers============================== } }
Dorothy Sorenson Microscopy and Image-analysis Laboratory Department of Cell and Developmental Biology University Of Michigan Medical School A807 BSRB 109 Zina Pitcher Place Ann Arbor, MI 48109-2200 (734)763-1170 FAX (734)763-1166
I have a question regarding to characteristic X-Ray K (alfa) and K (beta) lines intensities ratio. Books on X-Ray, like Cullity, states that the ratio between K (alfa1) and K (beta1) lines is 5:1. However Goldstein in his book on X-Ray microanalysis states that the ratio between intensities of K (alfa) and K (beta) is 10:1. Both books are considered like a Bible one in X-ray diffraction another in X-Ray microanalysis, so it can't be a simple discrepancy.
Thanks, Sergey
==============================Original Headers============================== 6, 19 -- From sergey-at-seas.ucla.edu Wed Sep 5 12:53:58 2007 6, 19 -- Received: from harbor.seas.ucla.edu (harbor.seas.ucla.edu [164.67.100.71]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85HrvqN005596 6, 19 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 12:53:57 -0500 6, 19 -- Received: (from root-at-localhost) 6, 19 -- by harbor.seas.ucla.edu (8.8.8/8.8.8(seas-smtp30-0010)) id KAA12530 6, 19 -- for microscopy-at-microscopy.com; Wed, 5 Sep 2007 10:53:57 -0700 (PDT) 6, 19 -- Received: from Beam.seas.ucla.edu (beam.seas.ucla.edu [128.97.83.27]) 6, 19 -- by harbor.seas.ucla.edu (8.8.8/8.8.8(seasall)-prefilter-007) with ESMTP id KAA12466 6, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 10:53:55 -0700 (PDT) 6, 19 -- Message-Id: {6.2.0.14.2.20070905094954.01d82f58-at-pop.seas.ucla.edu} 6, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 6, 19 -- Date: Wed, 05 Sep 2007 09:53:46 -0800 6, 19 -- To: Microscopy {Microscopy-at-microscopy.com} 6, 19 -- From: Sergey Prikhodko {sergey-at-seas.ucla.edu} 6, 19 -- Subject: K (alfa) and K (beta) ratio 6, 19 -- Mime-Version: 1.0 6, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 19 -- X-Virus-Scanned: by AMaViS Sophie/Sophos ==============================End of - Headers==============================
I haven't checked this, but are you comparing apples to apples? You ask about the ratio of Ka1 to Kb1 in Cullity and then Ka to Kb in Goldstein. Ka includes both Ka1 and Ka2, etc. I believe that you can look up relative intensities in the International X-ray Tables.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: sergey-at-seas.ucla.edu [mailto:sergey-at-seas.ucla.edu] Sent: Wednesday, September 05, 2007 10:56 AM To: Walck-at-SouthBayTech.com
Dear all:
I have a question regarding to characteristic X-Ray K (alfa) and K (beta) lines intensities ratio. Books on X-Ray, like Cullity, states that the ratio between K (alfa1) and K (beta1) lines is 5:1. However Goldstein in his book on X-Ray microanalysis states that the ratio between intensities of K (alfa) and K (beta) is 10:1. Both books are considered like a Bible one in X-ray diffraction another in X-Ray microanalysis, so it can't be a simple discrepancy.
Thanks, Sergey
==============================Original Headers============================== 6, 19 -- From sergey-at-seas.ucla.edu Wed Sep 5 12:53:58 2007 6, 19 -- Received: from harbor.seas.ucla.edu (harbor.seas.ucla.edu [164.67.100.71]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85HrvqN005596 6, 19 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 12:53:57 -0500 6, 19 -- Received: (from root-at-localhost) 6, 19 -- by harbor.seas.ucla.edu (8.8.8/8.8.8(seas-smtp30-0010)) id KAA12530 6, 19 -- for microscopy-at-microscopy.com; Wed, 5 Sep 2007 10:53:57 -0700 (PDT) 6, 19 -- Received: from Beam.seas.ucla.edu (beam.seas.ucla.edu [128.97.83.27]) 6, 19 -- by harbor.seas.ucla.edu (8.8.8/8.8.8(seasall)-prefilter-007) with ESMTP id KAA12466 6, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 10:53:55 -0700 (PDT) 6, 19 -- Message-Id: {6.2.0.14.2.20070905094954.01d82f58-at-pop.seas.ucla.edu} 6, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 6, 19 -- Date: Wed, 05 Sep 2007 09:53:46 -0800 6, 19 -- To: Microscopy {Microscopy-at-microscopy.com} 6, 19 -- From: Sergey Prikhodko {sergey-at-seas.ucla.edu} 6, 19 -- Subject: K (alfa) and K (beta) ratio 6, 19 -- Mime-Version: 1.0 6, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 19 -- X-Virus-Scanned: by AMaViS Sophie/Sophos ==============================End of - Headers==============================
==============================Original Headers============================== 15, 22 -- From walck-at-southbaytech.com Wed Sep 5 13:24:54 2007 15, 22 -- Received: from flpi101.prodigy.net (flpi101.sbcis.sbc.com [207.115.20.70]) 15, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85IOrR3018374 15, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 13:24:53 -0500 15, 22 -- X-ORBL: [64.169.217.123] 15, 22 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) 15, 22 -- by flpi101.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id l85IODJ5008824; 15, 22 -- Wed, 5 Sep 2007 11:24:13 -0700 15, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} 15, 22 -- To: {sergey-at-seas.ucla.edu} 15, 22 -- Cc: {Microscopy-at-microscopy.com} 15, 22 -- Subject: RE: [Microscopy] K (alfa) and K (beta) ratio 15, 22 -- Date: Wed, 5 Sep 2007 11:28:11 -0700 15, 22 -- Message-ID: {010a01c7efea$840c8ff0$7801a8c0-at-dynamicbl8uno3} 15, 22 -- MIME-Version: 1.0 15, 22 -- Content-Type: text/plain; 15, 22 -- charset="us-ascii" 15, 22 -- Content-Transfer-Encoding: 7bit 15, 22 -- X-Mailer: Microsoft Office Outlook 11 15, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 15, 22 -- Thread-Index: Acfv5hXDqnK7QxwPTrm00YsCwm7mlgAA08xw 15, 22 -- In-Reply-To: {200709051756.l85HuRqi011116-at-ns.microscopy.com} ==============================End of - Headers==============================
I have found that when I image a recently failed W filament, it appears hollow where it failed (these are gently used filaments, with months and months of service). I have placed 6 images on a web page for you to view if interested. I would be interested in comments how this (hollowing) phenomenon occurs.
www.geology.wisc.edu/~johnf/filament.html
Thanks
John F -- ======================================================== John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480 Cameron Electron Microprobe Lab lab: (608) 265-4798 Dept of Geology & Geophysics fax: (608) 262-0693 University of Wisconsin home: (608) 274-2245 1215 West Dayton St. email: johnf-at-geology.wisc.edu Madison, WI 53706 amateur radio: WA3BTA Personal http://www.geology.wisc.edu/~johnf/ Probe lab http://www.geology.wisc.edu/~johnf/sx51.html Probe Sign Up Calender: http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi
"The first rule of all intelligent tinkering is to save every cog and wheel." -- Aldo Leopold
"For a successful technology, reality must take precedence over public relations, for Nature cannot be fooled." -- Richard P. Feynman
==============================Original Headers============================== 6, 24 -- From johnf-at-geology.wisc.edu Wed Sep 5 13:27:04 2007 6, 24 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85IR2GX022876 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 13:27:04 -0500 6, 24 -- Received: from localhost (localhost [127.0.0.1]) 6, 24 -- by localhost (Postfix) with ESMTP id 26A2820D04 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 13:27:02 -0500 (CDT) 6, 24 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) 6, 24 -- by localhost (geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 6, 24 -- with ESMTP id 25784-05 for {microscopy-at-microscopy.com} ; 6, 24 -- Wed, 5 Sep 2007 13:26:53 -0500 (CDT) 6, 24 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57]) 6, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 6, 24 -- (No client certificate requested) 6, 24 -- by ice.geology.wisc.edu (Postfix) with ESMTP id 8D75720D06 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 13:26:53 -0500 (CDT) 6, 24 -- Mime-Version: 1.0 6, 24 -- Message-Id: {p06230912c304a5223979-at-[144.92.206.57]} 6, 24 -- Date: Wed, 5 Sep 2007 13:26:50 -0500 6, 24 -- To: microscopy-at-microscopy.com 6, 24 -- From: John Fournelle {johnf-at-geology.wisc.edu} 6, 24 -- Subject: Images of worn thru W filament 6, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 6, 24 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu ==============================End of - Headers==============================
First of all, yes my question was not absolutely correct because, as you mentioned I'm (actually Cullity and Goldstein) comparing slightly different things, but I guess it must be some other answer to my question. My understanding is that input of alfa 2 line will never make ratio of K alfa and K beta lines changed from 10:1 to 5:1.
Sergey
==============================Original Headers============================== 5, 19 -- From sergey-at-seas.ucla.edu Wed Sep 5 14:38:11 2007 5, 19 -- Received: from whittier.seas.ucla.edu (whittier.seas.ucla.edu [164.67.100.80]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85JcBwD011659 5, 19 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 14:38:11 -0500 5, 19 -- Received: (from root-at-localhost) 5, 19 -- by whittier.seas.ucla.edu (8.8.8/8.8.8(seas-smtp30-0010)) id MAA21099 5, 19 -- for microscopy-at-microscopy.com; Wed, 5 Sep 2007 12:38:10 -0700 (PDT) 5, 19 -- Received: from Beam.seas.ucla.edu (beam.seas.ucla.edu [128.97.83.27]) 5, 19 -- by whittier.seas.ucla.edu (8.8.8/8.8.8(seasall)-prefilter-007) with ESMTP id MAA21065 5, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 12:38:08 -0700 (PDT) 5, 19 -- Message-Id: {6.2.0.14.2.20070905112927.01de32f8-at-pop.seas.ucla.edu} 5, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 5, 19 -- Date: Wed, 05 Sep 2007 11:37:57 -0800 5, 19 -- To: Microscopy {Microscopy-at-microscopy.com} 5, 19 -- From: Sergey Prikhodko {sergey-at-seas.ucla.edu} 5, 19 -- Subject: RE: [Microscopy] K (alfa) and K (beta) ratio 5, 19 -- Mime-Version: 1.0 5, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 5, 19 -- X-Virus-Scanned: by AMaViS Sophie/Sophos ==============================End of - Headers==============================
I think Cullity and Goldstein were possibly more specific. The ratio has to be Z and other condition specific. For example, the count is not only related to the initial localized X-ray yield (Z and primarily energy) but also to the AF. You can get no Kb and a ratio of close to infinity for many light elements, right?
-cni
-----Original Message----- X-from: sergey-at-seas.ucla.edu [mailto:sergey-at-seas.ucla.edu] Sent: Wednesday, September 05, 2007 3:40 PM To: cni-at-UDel.Edu
Hi Scott,
First of all, yes my question was not absolutely correct because, as you mentioned I'm (actually Cullity and Goldstein) comparing slightly different things, but I guess it must be some other answer to my question. My understanding is that input of alfa 2 line will never make ratio of K alfa and K beta lines changed from 10:1 to 5:1.
Sergey
==============================Original Headers============================== 5, 19 -- From sergey-at-seas.ucla.edu Wed Sep 5 14:38:11 2007 5, 19 -- Received: from whittier.seas.ucla.edu (whittier.seas.ucla.edu [164.67.100.80]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85JcBwD011659 5, 19 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 14:38:11 -0500 5, 19 -- Received: (from root-at-localhost) 5, 19 -- by whittier.seas.ucla.edu (8.8.8/8.8.8(seas-smtp30-0010)) id MAA21099 5, 19 -- for microscopy-at-microscopy.com; Wed, 5 Sep 2007 12:38:10 -0700 (PDT) 5, 19 -- Received: from Beam.seas.ucla.edu (beam.seas.ucla.edu [128.97.83.27]) 5, 19 -- by whittier.seas.ucla.edu (8.8.8/8.8.8(seasall)-prefilter-007) with ESMTP id MAA21065 5, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 12:38:08 -0700 (PDT) 5, 19 -- Message-Id: {6.2.0.14.2.20070905112927.01de32f8-at-pop.seas.ucla.edu} 5, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 5, 19 -- Date: Wed, 05 Sep 2007 11:37:57 -0800 5, 19 -- To: Microscopy {Microscopy-at-microscopy.com} 5, 19 -- From: Sergey Prikhodko {sergey-at-seas.ucla.edu} 5, 19 -- Subject: RE: [Microscopy] K (alfa) and K (beta) ratio 5, 19 -- Mime-Version: 1.0 5, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 5, 19 -- X-Virus-Scanned: by AMaViS Sophie/Sophos ==============================End of - Headers==============================
==============================Original Headers============================== 18, 27 -- From cni-at-udel.edu Wed Sep 5 14:57:50 2007 18, 27 -- Received: from md4.nss.udel.edu (md4.nss.udel.edu [128.175.1.14]) 18, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85Jvohh023827 18, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 14:57:50 -0500 18, 27 -- Received: from Suba (em076-119.engg.udel.edu [128.175.119.76]) 18, 27 -- by md4.nss.udel.edu (MOS 3.8.4-GA) 18, 27 -- with ESMTP id ETW67608; 18, 27 -- Wed, 5 Sep 2007 15:57:49 -0400 (EDT) 18, 27 -- From: "Chaoying Ni" {cni-at-udel.edu} 18, 27 -- To: {sergey-at-seas.ucla.edu} 18, 27 -- Cc: {Microscopy-at-microscopy.com} 18, 27 -- Subject: RE: [Microscopy] RE: K (alfa) and K (beta) ratio 18, 27 -- Date: Wed, 5 Sep 2007 15:58:03 -0400 18, 27 -- Organization: University of Delaware 18, 27 -- Message-ID: {000601c7eff7$11a0a610$4c77af80-at-Suba} 18, 27 -- MIME-Version: 1.0 18, 27 -- Content-Type: text/plain; 18, 27 -- charset="us-ascii" 18, 27 -- X-Priority: 3 (Normal) 18, 27 -- X-MSMail-Priority: Normal 18, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 18, 27 -- Thread-Index: Acfv9H2AKTe0vh/lRhO2slLftAY30wAANUCg 18, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 18, 27 -- Importance: Normal 18, 27 -- In-Reply-To: {200709051939.l85JdZLB014183-at-ns.microscopy.com} 18, 27 -- Content-Transfer-Encoding: 8bit 18, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l85Jvohh023827 ==============================End of - Headers==============================
Following on from Garnet's reply have at look at the paper:
The axonal transmission of Herpes simplex virus to epidermal cells: a novel use of the freeze substitution technique applied to explant cultures retained on cover slips.
Journal of Microscopy Vol 192, Pt 11998 (pages 69 -72)
Although the method dexribed is used with thermonox and Lowicryl HM20 resin, the procedure works as well with with epoxy resin and Lowicryl HM20 resin.
I like the idea of using a light vacuum as suggested by Garnet, I haven't tried that.
Regards
Allan
Allan Mitchell Otago Centre for Electron Microscopy Department of Anatomy and Structural Biology School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254
==============================Original Headers============================== 15, 21 -- From allan.mitchell-at-stonebow.otago.ac.nz Wed Sep 5 16:06:00 2007 15, 21 -- Received: from mailhub2.otago.ac.nz (mailhub2.otago.ac.nz [139.80.64.247]) 15, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85L5wZf004668 15, 21 -- for {microscopy-at-msa.microscopy.com} ; Wed, 5 Sep 2007 16:05:59 -0500 15, 21 -- Received: from galadriel.otago.ac.nz (galadriel.otago.ac.nz [139.80.64.213]) 15, 21 -- by mailhub2.otago.ac.nz (8.13.8/8.13.8) with ESMTP id l85L5u9Y009512; 15, 21 -- Thu, 6 Sep 2007 09:05:56 +1200 15, 21 -- Received: from allan.otago.ac.nz ([139.80.40.92]) 15, 21 -- by galadriel.otago.ac.nz with esmtp (Exim 4.63) 15, 21 -- (envelope-from {allan.mitchell-at-stonebow.otago.ac.nz} ) 15, 21 -- id 1IT22e-0005K1-0a; Thu, 06 Sep 2007 09:04:04 +1200 15, 21 -- Mime-Version: 1.0 (Apple Message framework v752.2) 15, 21 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 15, 21 -- Message-Id: {A92D6218-D1B9-4E7F-9EA2-7096D64BD249-at-stonebow.otago.ac.nz} 15, 21 -- Cc: peta.clode-at-uwa.edu.au 15, 21 -- Content-Transfer-Encoding: 7bit 15, 21 -- From: Allan Mitchell {allan.mitchell-at-stonebow.otago.ac.nz} 15, 21 -- Subject: [Microscopy] TEM - cells on thermonox 15, 21 -- Date: Thu, 6 Sep 2007 09:05:54 +1200 15, 21 -- To: microscopy-at-msa.microscopy.com 15, 21 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
This happens quite commonly when examining monolayers. Several things to try:
1. Use a supporting substrate (Formvar/carbon for example) to collect the sections.
2. Grow the cells on epoxy substrate (if they will attach). Then layer on the epoxy for the embedding and the cells should be sandwiched.
JB
} We are using TEM to look at cells that are grown on thermonox } coverslips - however, when we try to section them for TEM the } thermonox pulls away from the epoxy and we are left with our cells } sitting along the edge of the section, which is largely unstable in } the TEM. As such we have to remove the thermonox and re-embed them } before sectioning, which rather defeats the purpose of using } thermonox. } } Does anyone have any tips for preparing cells on thermonox for TEM? } } -- } } ***************************************** } } Dr. Martin Saunders, } Deputy Director and Senior Lecturer, } Centre for Microscopy, Characterisation and Analysis, } M010, } The University of Western Australia, } Crawley, } Western Australia 6009, } Australia. } } Phone: +61 8 6488 8092 } Fax: +61 8 6488 1087 } E-mail: Martin.Saunders-at-uwa.edu.au } CRICOS Provider No. 00126G } } ***************************************** } } } ==============================Original Headers============================== } 13, 31 -- From martin-at-cmm.uwa.edu.au Wed Sep 5 00:21:12 2007 } 13, 31 -- Received: from asclepius2.uwa.edu.au } (asclepius3.uwa.edu.au [130.95.128.60]) } 13, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l855LBZ7012510 } 13, 31 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 5 Sep 2007 } 00:21:12 -0500 } 13, 31 -- Received: from kas30pipe.localhost (localhost.localdomain } [127.0.0.1]) } 13, 31 -- by panacea.uwa.edu.au (Postfix) with ESMTP id B11918810E } 13, 31 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 5 Sep 2007 } 13:21:09 +0800 (WST) } 13, 31 -- Received: from panacea (localhost.localdomain [127.0.0.1]) } 13, 31 -- by panacea.prekas (Postfix) with SMTP id B153D882A9 } 13, 31 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 5 Sep 2007 } 13:21:06 +0800 (WST) } 13, 31 -- X-UWA-Client-IP: 130.95.124.130 (UWA) } 13, 31 -- Received: from [130.95.124.130] (awsjoff.cmm.uwa.edu.au } [130.95.124.130]) } 13, 31 -- by panacea.input (Postfix) with ESMTP id 1B51B88254 } 13, 31 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 5 Sep 2007 } 13:21:05 +0800 (WST) } 13, 31 -- Mime-Version: 1.0 } 13, 31 -- Message-Id: {a06240864c303ed41f85c-at-[130.95.124.130]} } 13, 31 -- Date: Wed, 5 Sep 2007 13:21:38 +0800 } 13, 31 -- To: Microscopy-at-MSA.Microscopy.Com } 13, 31 -- From: Martin Saunders {martin-at-cmm.uwa.edu.au} } 13, 31 -- Subject: TEM - cells on thermonox } 13, 31 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } 13, 31 -- X-SpamTest-Envelope-From: martin-at-cmm.uwa.edu.au } 13, 31 -- X-SpamTest-Group-ID: 00000000 } 13, 31 -- X-SpamTest-Info: Profiles 1433 [September 4 2007] } 13, 31 -- X-SpamTest-Info: {HEADERS: header Content-Type found } without required header Content-Transfer-Encoding} } 13, 31 -- X-SpamTest-Info: {MSGID: id-right missed} } 13, 31 -- X-SpamTest-Method: probable } 13, 31 -- X-SpamTest-Rate: 60 } 13, 31 -- X-SpamTest-Status: UWA Probable } 13, 31 -- X-SpamTest-Status-Extended: probable_spam } 13, 31 -- X-SpamTest-Version: SMTP-Filter Version 3.0.0 [0278], KAS30/Release } ==============================End of - Headers==============================
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
You are right. Indeed the ratio depends on atomic number, but I expect if this dependence was significantly different to light elements compare to heavy ones any of mentioned above books would probably specify it, but they don't. At the same time if this ratio could change in two times (from 10:1 to 5:1) for different elements there wouldn't be repayable approach (as Goldstein suggest) to use ratio 10:1 for identification of elements which I believe most of spectroscopists follow.
Sergey
==============================Original Headers============================== 6, 19 -- From sergey-at-seas.ucla.edu Wed Sep 5 16:41:54 2007 6, 19 -- Received: from whittier.seas.ucla.edu (whittier.seas.ucla.edu [164.67.100.80]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85LfrLh028633 6, 19 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 16:41:53 -0500 6, 19 -- Received: (from root-at-localhost) 6, 19 -- by whittier.seas.ucla.edu (8.8.8/8.8.8(seas-smtp30-0010)) id OAA12252 6, 19 -- for microscopy-at-microscopy.com; Wed, 5 Sep 2007 14:41:49 -0700 (PDT) 6, 19 -- Received: from Beam.seas.ucla.edu (beam.seas.ucla.edu [128.97.83.27]) 6, 19 -- by whittier.seas.ucla.edu (8.8.8/8.8.8(seasall)-prefilter-007) with ESMTP id OAA12235 6, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 14:41:48 -0700 (PDT) 6, 19 -- Message-Id: {6.2.0.14.2.20070905134036.03573e80-at-pop.seas.ucla.edu} 6, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 6, 19 -- Date: Wed, 05 Sep 2007 13:41:39 -0800 6, 19 -- To: Microscopy {Microscopy-at-microscopy.com} 6, 19 -- From: Sergey Prikhodko {sergey-at-seas.ucla.edu} 6, 19 -- Subject: Re: [Microscopy] K (alfa) and K (beta) ratio 6, 19 -- Mime-Version: 1.0 6, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 19 -- X-Virus-Scanned: by AMaViS Sophie/Sophos ==============================End of - Headers==============================
I think that Tom's answer is the closer one to reality trying to explain the situation based on the way how signal is detected for both techniques, but sorry again I'm not completely satisfied with his explanation. For example, WDS (technique I'm not really familiar with) separates peaks based on their diffraction angle, but when you look through the spectra in Goldstein (second edition page 361, for example, Al K (alfa) and Al K (beta)) the ratio is far from 5:1, how one could expect assuming that Tom's explanation is right. I'm pretty sure a lot of people here are dealing with WDS. What is the regular ratio between intensities of K (alfa) and K (beta) lines one can experimentally measure in WDS?
Thanks, Sergey
==============================Original Headers============================== 6, 19 -- From sergey-at-seas.ucla.edu Wed Sep 5 17:35:25 2007 6, 19 -- Received: from harbor.seas.ucla.edu (harbor.seas.ucla.edu [164.67.100.71]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85MZPtr009075 6, 19 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 17:35:25 -0500 6, 19 -- Received: (from root-at-localhost) 6, 19 -- by harbor.seas.ucla.edu (8.8.8/8.8.8(seas-smtp30-0010)) id PAA08280 6, 19 -- for microscopy-at-microscopy.com; Wed, 5 Sep 2007 15:35:25 -0700 (PDT) 6, 19 -- Received: from Beam.seas.ucla.edu (beam.seas.ucla.edu [128.97.83.27]) 6, 19 -- by harbor.seas.ucla.edu (8.8.8/8.8.8(seasall)-prefilter-007) with ESMTP id PAA08212 6, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 15:35:22 -0700 (PDT) 6, 19 -- Message-Id: {6.2.0.14.2.20070905143324.01e32998-at-pop.seas.ucla.edu} 6, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 6, 19 -- Date: Wed, 05 Sep 2007 14:35:13 -0800 6, 19 -- To: Microscopy {Microscopy-at-microscopy.com} 6, 19 -- From: Sergey Prikhodko {sergey-at-seas.ucla.edu} 6, 19 -- Subject: RE: [Microscopy] RE: K (alfa) and K (beta) ratio 6, 19 -- Mime-Version: 1.0 6, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 19 -- X-Virus-Scanned: by AMaViS Sophie/Sophos ==============================End of - Headers==============================
Could it be possible that the filament doesn't evaporate evenly? For instance, if one area gets slightly thinner than another, then evaporates off in a "bubble" until the final strand breaks. Imagine blowing a bubble in a warm taffy string- it would weaken the overall string until it broke, leaving the concave inside surfaces of the bubble on either side of the string.
Granted, this is just a guess based on my experience with other materials. It could be completely wrong. You have me interested, though!
--Justin A. Kraft
On 9/5/07, johnf-at-geology.wisc.edu {johnf-at-geology.wisc.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I have found that when I image a recently failed W filament, it } appears hollow where it failed (these are gently used filaments, with } months and months of service). I have placed 6 images on a web page } for you to view if interested. I would be interested in comments how } this (hollowing) phenomenon occurs. } } www.geology.wisc.edu/~johnf/filament.html } } Thanks } } John F } -- } ======================================================== } John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480 } Cameron Electron Microprobe Lab lab: (608) 265-4798 } Dept of Geology & Geophysics fax: (608) 262-0693 } University of Wisconsin home: (608) 274-2245 } 1215 West Dayton St. email: johnf-at-geology.wisc.edu } Madison, WI 53706 amateur radio: WA3BTA } Personal http://www.geology.wisc.edu/~johnf/ } Probe lab http://www.geology.wisc.edu/~johnf/sx51.html } Probe Sign Up Calender: } http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi } } "The first rule of all intelligent tinkering is to save every cog and } wheel." -- Aldo Leopold } } "For a successful technology, reality must take precedence over } public relations, for Nature cannot be fooled." -- Richard P. } Feynman } } ==============================Original Headers============================== } 6, 24 -- From johnf-at-geology.wisc.edu Wed Sep 5 13:27:04 2007 } 6, 24 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14]) } 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85IR2GX022876 } 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 13:27:04 -0500 } 6, 24 -- Received: from localhost (localhost [127.0.0.1]) } 6, 24 -- by localhost (Postfix) with ESMTP id 26A2820D04 } 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 13:27:02 -0500 (CDT) } 6, 24 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) } 6, 24 -- by localhost (geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) } 6, 24 -- with ESMTP id 25784-05 for {microscopy-at-microscopy.com} ; } 6, 24 -- Wed, 5 Sep 2007 13:26:53 -0500 (CDT) } 6, 24 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57]) } 6, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) } 6, 24 -- (No client certificate requested) } 6, 24 -- by ice.geology.wisc.edu (Postfix) with ESMTP id 8D75720D06 } 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 13:26:53 -0500 (CDT) } 6, 24 -- Mime-Version: 1.0 } 6, 24 -- Message-Id: {p06230912c304a5223979-at-[144.92.206.57]} } 6, 24 -- Date: Wed, 5 Sep 2007 13:26:50 -0500 } 6, 24 -- To: microscopy-at-microscopy.com } 6, 24 -- From: John Fournelle {johnf-at-geology.wisc.edu} } 6, 24 -- Subject: Images of worn thru W filament } 6, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } 6, 24 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu } ==============================End of - Headers============================== }
Further to John's comments, a number years ago in one of the CSIRO laboratories a staff member died when they entered the nitrogen fridge room in the evening. Several factors contributed to the accident. It was 12 hours before he was found.
This incident caused a review of all liquid nitrogen and gases use across our organisation. In our lab we use the blow off from a 2000 litre liquid nitrogen tank to provide gas for valves, cleaning etc. This can generate enough gas to fill the laboratory several times over! Several changes were made.
A valve was fitted to the main nitrogen gas line so that if there was a rapid drop in pressure in the line the gas would be shut off.
The lab was fitted with multiple oxygen sensors with a monitoring station outside the lab. These are checked and calibrated every 6 months.
Electronic locks were fitted to the doors and linked to the sensors. If the oxygen level drops below 19.5% a VERY loud alarm sounds and the doors lock to prevent entry. You can and must exit when the alarm sounds. The only people who can open the doors once they have been in an alarmed state are our emergency response crew who have to use personal oxygen monitors and breathing apparatus to enter.
We also have procedures in place if you need to enter the laboratory after normal working hours or on weekends.
It took a little while to iron out the bugs but it works well now.
Just my 2 cents worth!
Cheers.
Colin Veitch
Electron Microscopist CSIRO Textile and Fibre Technology PO Box 21, BELMONT, Vic. 3216. Australia. colin.veitch-at-csiro.au http://www.tft.csiro.au
The information contained in this e-mail message may be privileged or confidential information. If you are not an intended recipient, you may not copy, distribute or take any action in reliance on it. If you have received this message in error, please telephone CSIRO Textile and Fibre Technology on +61 3 5246 4000.
-----Original Message----- X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com] Sent: Wednesday, 5 September 2007 6:24 AM To: Veitch, Colin (TFT, Geelong)
Back when we still had a darkroom with rotary doors, the safety people asked us to put in extra heavy duty hoses for the nitrogen guns that we used to blow off dust. We thought they were going overboard at first when they chose 12,000 psi rated brake hoses, but this was judged much cheaper than oxygen sensors, and when we thought about the scenario they were designed to prevent, we thought it was OK. What is the scenario? Imagine a hose fails in the middle of the night or weekend. The room fills with nitrogen, displacing all of the air. A person entering the darkroom through the rotary doors would be breathing 100% nitrogen, and would pass out after taking one breath. Nobody on the outside would see them. Anyone entering the darkroom would suffer the same fate. These would become fatalities. Basically, the consequences of a nitrogen leak were deemed so severe that 12,000 psi rated brake hose nitrogen lines seemed reasonable when used in darkroom with rotary doors.
John Mardinly Intel
This is not an opinion of Intel Corporation.
-----Original Message----- X-from: nholson-at-ucsd.edu [mailto:nholson-at-ucsd.edu] Sent: Tuesday, September 04, 2007 12:42 PM To: Mardinly, John
-- Along this same line of thought, does anyone know of any in-room sensor that can be purchased that emits an alarm if oxygen levels get low. I am thinking of something that works like either a smoke alarm or a carbon monoxide detector. We need to use ethane gas for our cryo procedures and the safety people are causing an uproar about that. These people tend to make a "one size fits all" type of regulation that often has little relevance to specific situations. If anyone has any specific product I would appreciate the info.
Norm Olson
______________________________________________________________ Norm Olson Cryoelectron Microscopy Facilities Manager 1510 Bonner Hall Department of Chemistry & Biochemistry, MC-0378 University of California San Diego La Jolla, CA 92093-0378 nholson-at-ucsd.edu http://cryoem.ucsd.edu Cell: (858)220-2183 (858)822-6718 - Office; (858)534-5846 - Fax ______________________________________________________________
In the past, I used W filaments. As I recall, the electrons are boiled off of tip and as this happens, the W wire becomes thinner. When this happens, some point on the filament (near the tip) will experience high current density. Once this starts, the filament is basically a fuse and it blows. The weakest points are tri-grain boundaries. This is likely the spot you saw where the W was thin (high J) and tri-grain boundary.
There could be other explanations.
gary g.
At 10:28 AM 9/5/2007, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 6, 20 -- From gary-at-gaugler.com Wed Sep 5 17:57:56 2007 6, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l85MvtA0012131 6, 20 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 17:57:55 -0500 6, 20 -- Message-Id: {200709052257.l85MvtA0012131-at-ns.microscopy.com} 6, 20 -- Received: (qmail 21050 invoked from network); 5 Sep 2007 15:57:53 -0700 6, 20 -- Received: by simscan 1.1.0 ppid: 21035, pid: 21043, t: 0.2868s 6, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 6, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 6, 20 -- by qsmtp3 with SMTP; 5 Sep 2007 15:57:53 -0700 6, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 6, 20 -- Date: Wed, 05 Sep 2007 15:58:01 -0800 6, 20 -- To: johnf-at-geology.wisc.edu 6, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 6, 20 -- Subject: Re: [Microscopy] Images of worn thru W filament 6, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 6, 20 -- In-Reply-To: {200709051828.l85ISiI8027871-at-ns.microscopy.com} 6, 20 -- References: {200709051828.l85ISiI8027871-at-ns.microscopy.com} 6, 20 -- Mime-Version: 1.0 6, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-4267365A ==============================End of - Headers==============================
The purpose that many of us use the permanox for is to have the embedded cells easily separate from the dish. They can then be mounted on top of blank epon blocks for face-on sections or they can be "glued" together to get cross sections of the cells. From what you have described you wish the cross-sections. Others have suggested using the coated grids and that works about 85% of the time if one has very flat cells like fibroblasts since there is still some curling on many sections but if the cells are cuboidal then this works fine nearly 100%.
I had presented a paper at M&M 1977 that Hayat has published in his Principles and Techniques of Electron Microscopy 3rd and 4th editions This was before permanox and one needed to get the cells out of standard tissue culture dishes. Note: Some Epon substitutes melt these dishes. LX112 from Ladd works as well as the original Epon 812 that I was using then. I have not tried out the epon substitutes from all the suppliers. All seem to work equally well with Permanox.
Basically I would cure the epon for 24 hours at 60 degrees; cut it into small rectangles that would fit into standard embedding molds - about 3x4 mm; place one piece cells up into the mold that was half full of epon reserved from the previous day in the freezer+thawed to room temp. (same batch) then in coverslip fashion put a second piece on top of the first with the cell side down; fill the mold and put it into the oven for 2 days more. This produces a sandwich of two layers of cells close together. One can put two pieces from the same dish (twice the number of cells / section) or a control with an experimental specimen in the same mold (no difference in section thickness or staining) as long as one keeps track. I like the control on the bottom of the mold, then when I trim, I trim the control side flat and the experimental side with a wide angle so that it can be easily determined on the TEM which side is being examined.
Cells should be lined up perpendicular to the knife edge and the bottom of the block face should be trimmed so that there is no blank epon below the cells. This block face can be as high as 3 mm if your sectioning window allows but only 0.6 wide at the bottom and 0.4 or less at the top. If you look in Hayat, the drawing should be half as wide as it looks. Yes, I have done this many times.
Occasionally the stripes of epoxy will separate but usually one side will stick well and coated grids can still be used.
Good luck, Pat
Patricia Stranen Connelly Lead Technologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-65 connellyps-at-mail.nih.gov
==============================Original Headers============================== 12, 23 -- From connellyps-at-nhlbi.nih.gov Wed Sep 5 17:58:56 2007 12, 23 -- Received: from NIHCESSMTP2.hub.nih.gov (nihcessmtp2.hub.nih.gov [128.231.90.116]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85MwuFF014297 12, 23 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 17:58:56 -0500 12, 23 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 12, 23 -- Wed, 5 Sep 2007 18:58:51 -0400 12, 23 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.170]) with Microsoft Exchange Server HTTP-DAV ; 12, 23 -- Wed, 5 Sep 2007 22:58:51 +0000 12, 23 -- User-Agent: Microsoft-Entourage/11.3.6.070618 12, 23 -- Date: Wed, 05 Sep 2007 18:57:28 -0400 12, 23 -- Subject: [Microscopy] Re: TEM - cells on thermonox 12, 23 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 12, 23 -- To: {peta.clode-at-uwa.edu.au} , 12, 23 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 12, 23 -- Message-ID: {C304AC98.BD9%connellyps-at-nhlbi.nih.gov} 12, 23 -- Thread-Topic: [Microscopy] Re: TEM - cells on thermonox 12, 23 -- Thread-Index: AcfwECHEYA/WwlwDEdyaKwANk2Yv1A== 12, 23 -- Mime-version: 1.0 12, 23 -- Content-type: text/plain; 12, 23 -- charset="ISO-8859-1" 12, 23 -- X-OriginalArrivalTime: 05 Sep 2007 22:58:51.0994 (UTC) FILETIME=[53D4B3A0:01C7F010] 12, 23 -- Content-Transfer-Encoding: 8bit 12, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l85MwuFF014297 ==============================End of - Headers==============================
Perhaps the truth is midway between the two sources (Cullity and Goldstein), but I expect a lot has to do with the difference between WDS and EDS. The Ka1 peak is about twice the height of the Ka2 peak. EDS will not resolve the two peaks but will simply measure the sum of both. Thus, if Ka1 is 5x the Kb1 peak and if Ka1 is 1.5x the Ka2 peak, Ka (Ka1+Ka2 by EDS) should be 7.5x the Kb1 peak. It's not the 10x that Goldstein quoted, but it's in the right ballpark.
The element effect also comes into play. I used our Oxford EDS system to integrate the Ka and Kb peaks for Ca, Fe, and Ge for a 20 kV beam. The ratios of the net peak integrals were 9.3, 7.5, and 6.6, respectively. That is a substantial change across the range.
So what is the question again and why do you ask? How does it effect how we do our work?
I use the MLK markers or my systems to give a rough idea of what is present. I do not use them to determine if another element is hiding in a mess of peaks. I _do_ examine the residuals between fitted and original spectrum to make sure I have not missed an overlapping element. For the fitting, I use my own peak shapes as much as possible and I deal with the whole line series rather than with someone else's tabulated information.
Warren Straszheim
For Ca Window Range (keV) Gross Net % total CaKa 3.487 to 3.868 51768 48398 90.2 CaKb 3.888 to 4.148 7415 5189 9.7 Ka/Kb = 9.3
For Fe Label Range (keV) Gross Net % total FeKa 6.028 to 6.688 57080 53527 88.6 FeKb 6.787 to 7.367 9464 7184 11.9 Ka/Kb = 7.5
For Ge Window Range (keV) Gross Net % total GeKa 9.568 to 10.208 18785 17086 86.8 GeKb 10.748 to 11.267 3680 2600 13.2 Ka/Kb = 6.6
Ge peak heights Peak Gross Net Ka 1880 1840 Kb 300 262 Bkgd 38 -- Ka/Kb = 7.0 (close to ratio of the integrals)
Marker lines show Ka1 at 9.887 2520 cts Ka2 at 9.855 1260 cts Ka at 9.88 3780 cts
Kb1 at 10.982 360 cts Kb2 at 11.101 14 cts Kb at 11.00 370 cts
Ka1/Kb = 7.0 (close to ratio of the integrals) Ka/Kb = 10.2 (~50% higher than the Ka1/Kb ratio) Maybe Oxford choose to make the Ka1 marker the height of the combined Ka emission since the peak height in EDS will be the sum of the Ka1 and Ka2 line contributions.
-----Original Message----- X-from: sergey-at-seas.ucla.edu [mailto:sergey-at-seas.ucla.edu] Sent: Wednesday, September 05, 2007 12:54 PM To: wesaia-at-iastate.edu
Dear all:
I have a question regarding to characteristic X-Ray K (alfa) and K (beta) lines intensities ratio. Books on X-Ray, like Cullity, states that the ratio between K (alfa1) and K (beta1) lines is 5:1. However Goldstein in
his book on X-Ray microanalysis states that the ratio between intensities of K (alfa) and K (beta) is 10:1. Both books are considered like a Bible
one in X-ray diffraction another in X-Ray microanalysis, so it can't be a simple discrepancy.
Thanks, Sergey
==============================Original Headers============================== 22, 34 -- From wesaia-at-iastate.edu Wed Sep 5 18:40:05 2007 22, 34 -- Received: from mailhub-3.iastate.edu (mailhub-3.iastate.edu [129.186.140.13]) 22, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85Ne5RO003712 22, 34 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 5 Sep 2007 18:40:05 -0500 22, 34 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47]) 22, 34 -- by mailhub-3.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id l85Ne5nk006042 22, 34 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 5 Sep 2007 18:40:05 -0500 22, 34 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by devirus-10.iastate.edu with smtp 22, 34 -- id 3acf_92918dc0_5c08_11dc_9f48_00137253420a; 22, 34 -- Wed, 05 Sep 2007 18:34:40 -0500 22, 34 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 22, 34 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id l85NdxBF023868 22, 34 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 5 Sep 2007 18:39:59 -0500 22, 34 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 22, 34 -- Wed, 5 Sep 2007 18:40:00 -0500 22, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 22, 34 -- Content-class: urn:content-classes:message 22, 34 -- MIME-Version: 1.0 22, 34 -- Content-Type: text/plain; 22, 34 -- charset="US-ASCII" 22, 34 -- Subject: RE: [Microscopy] K (alfa) and K (beta) ratio 22, 34 -- Date: Wed, 5 Sep 2007 18:41:58 -0500 22, 34 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72701D82F8E-at-maire.eng.iastate.edu} 22, 34 -- X-MS-Has-Attach: 22, 34 -- X-MS-TNEF-Correlator: 22, 34 -- Thread-Topic: RE: [Microscopy] K (alfa) and K (beta) ratio 22, 34 -- Thread-Index: AcfwFllUB7LVmqp3Sp2ABQlzrq1vtA== 22, 34 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 22, 34 -- To: "MSA" {Microscopy-at-msa.microscopy.com} 22, 34 -- X-OriginalArrivalTime: 05 Sep 2007 23:40:00.0596 (UTC) FILETIME=[133B7940:01C7F016] 22, 34 -- X-PMX-Version: 5.3.1.294258, Antispam-Engine: 2.5.1.298604, Antispam-Data: 2007.9.5.162123 22, 34 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=7%, Report='__CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __pbl.spamhaus.org_TIMEOUT ' 22, 34 -- Content-Transfer-Encoding: 8bit 22, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l85Ne5RO003712 ==============================End of - Headers==============================
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Email: niels.de.jonge-at-vanderbilt.edu Name: Niels de Jonge
Organization: Vanderbilt University and Oak Ridge National Laboratory
Title-Subject: [Filtered] Post-doc and graduate positions in liquid and 3D STEM biomedical imaging at Vanderbilt University
Question: Post-doc and graduate positions are available for biologists, physicists and biophysicists at Vanderbilt University (Nashville, USA) in collaboration with Oak Ridge National Laboratory (Oak Ridge, USA) on liquid and 3D scanning transmission electron microscopy (STEM), new ways to achieve molecular level imaging of cells. Subjects range from physics of imaging in liquid, electron microscopy instrumentation, electron microscopy imaging with aberration corrected STEM, microfluidics, biochemistry of new molecular probes for liquid STEM, cell biology including specific labeling of proteins and molecular imaging. More information can be found on the website: https://www.mst.ornl.gov/Liquid3DSTEM
John, If you look at the way the W grains seem to be layered, it would appear to me that the failure simply occurred along the grain boundaries.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: johnf-at-geology.wisc.edu [mailto:johnf-at-geology.wisc.edu] Sent: Wednesday, September 05, 2007 2:30 PM To: kenconverse-at-qualityimages.biz
I have found that when I image a recently failed W filament, it appears hollow where it failed (these are gently used filaments, with months and months of service). I have placed 6 images on a web page for you to view if interested. I would be interested in comments how this (hollowing) phenomenon occurs.
www.geology.wisc.edu/~johnf/filament.html
Thanks
John F -- ======================================================== John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480 Cameron Electron Microprobe Lab lab: (608) 265-4798 Dept of Geology & Geophysics fax: (608) 262-0693 University of Wisconsin home: (608) 274-2245 1215 West Dayton St. email: johnf-at-geology.wisc.edu Madison, WI 53706 amateur radio: WA3BTA Personal http://www.geology.wisc.edu/~johnf/ Probe lab http://www.geology.wisc.edu/~johnf/sx51.html Probe Sign Up Calender: http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi
"The first rule of all intelligent tinkering is to save every cog and wheel." -- Aldo Leopold
"For a successful technology, reality must take precedence over public relations, for Nature cannot be fooled." -- Richard P. Feynman
==============================Original Headers============================== 6, 24 -- From johnf-at-geology.wisc.edu Wed Sep 5 13:27:04 2007 6, 24 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85IR2GX022876 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 13:27:04 -0500 6, 24 -- Received: from localhost (localhost [127.0.0.1]) 6, 24 -- by localhost (Postfix) with ESMTP id 26A2820D04 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 13:27:02 -0500 (CDT) 6, 24 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) 6, 24 -- by localhost (geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 6, 24 -- with ESMTP id 25784-05 for {microscopy-at-microscopy.com} ; 6, 24 -- Wed, 5 Sep 2007 13:26:53 -0500 (CDT) 6, 24 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57]) 6, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 6, 24 -- (No client certificate requested) 6, 24 -- by ice.geology.wisc.edu (Postfix) with ESMTP id 8D75720D06 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 13:26:53 -0500 (CDT) 6, 24 -- Mime-Version: 1.0 6, 24 -- Message-Id: {p06230912c304a5223979-at-[144.92.206.57]} 6, 24 -- Date: Wed, 5 Sep 2007 13:26:50 -0500 6, 24 -- To: microscopy-at-microscopy.com 6, 24 -- From: John Fournelle {johnf-at-geology.wisc.edu} 6, 24 -- Subject: Images of worn thru W filament 6, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 6, 24 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu ==============================End of - Headers==============================
==============================Original Headers============================== 21, 25 -- From kenconverse-at-qualityimages.biz Thu Sep 6 08:19:51 2007 21, 25 -- Received: from mta9.adelphia.net (mta9.adelphia.net [68.168.78.199]) 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l86DJpKK010484 21, 25 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 6 Sep 2007 08:19:51 -0500 21, 25 -- Received: from Ken ([72.227.100.25]) by mta9.adelphia.net 21, 25 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 21, 25 -- id {20070906131950.BODW4730.mta9.adelphia.net-at-Ken} ; 21, 25 -- Thu, 6 Sep 2007 09:19:50 -0400 21, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 21, 25 -- To: {johnf-at-geology.wisc.edu} , "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 21, 25 -- Subject: RE: [Microscopy] Images of worn thru W filament 21, 25 -- Date: Thu, 6 Sep 2007 09:19:41 -0400 21, 25 -- Message-ID: {004101c7f088$95c124b0$6401a8c0-at-Ken} 21, 25 -- MIME-Version: 1.0 21, 25 -- Content-Type: text/plain; 21, 25 -- charset="us-ascii" 21, 25 -- X-Priority: 3 (Normal) 21, 25 -- X-MSMail-Priority: Normal 21, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 21, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 21, 25 -- Importance: Normal 21, 25 -- Thread-Index: AcfwBc5MrG3swpEsSQqeZ06/10qMFAAgoCSQ 21, 25 -- In-Reply-To: {200709051829.l85ITiGk031054-at-ns.microscopy.com} 21, 25 -- Content-Transfer-Encoding: 8bit 21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l86DJpKK010484 ==============================End of - Headers==============================
John, Those are quite remarkable images and I don't claim to have the explanation. However, the following observations might be relevant:
First of all, that certainly is a "gently used" filament. That's clear from the morphology of the wire surface, and I think this "gentle failure" somehow plays a role in what you are seeing. I think this also rules out an initial defect in the filament wire (e.g., an internal void) since this would lead to an abrupt failure at the location of the void.
It's well known that the typical end-of-life scenario for a tungsten filament is a self-accelerating process. Through most of its life, the end of the filament is at a fairly uniform temperature and evaporation of metal is rather uniform across the tip of the filament. However, some location will inevitably be thinner than the rest (generally at the side of the V-tip where the wire has been stretched in the bending process) and this thinner point will run a little hotter and evaporate a little faster. The thinner it gets, the higher the local resistance, the higher the temperature (relative to the rest of the filament) and the greater the localized evaporation. At one point in my life I spent a couple of months monitoring filaments through the lens of an optical pyrometer and I can confirm what others have also noted, that it is hard to detect any difference in wire diameter till very near the end, and the final stages of thinning and failure go very quickly. On a couple of occasions, I had the good fortune of observing the final death throes. What I saw was a molten blob of metal that bridged the gap for a brief period and sort of "danced" there before it separated and the characteristic ball-shaped ends were formed. I think that the observed mechanical oscillation also plays a role in the abrupt death since the differential heating becomes even more extreme where the molten blob necks down so that the final separation is almost "explosive." But that scenario isn't what happened here, IMHO.
A lesser known, but potentially relevant fact is that the predominant mechanism for dissipation of the filament's heat is radiation -- as I recall from the mathematical modeling I did long ago, something like 2/3 of the heat generated in the tip of a typically-operated tungsten filament is dissipated as light (conduction accounts for the rest -- energy dissipation via electron emission is negligible). Since light emission is a surface effect, it stands to reason that the surface of the wire is ever-so-slightly cooler than the interior. Consequently, one would *expect* that melting would begin in the core of the wire, but under normal circumstances this quickly involves the surface too. (Somebody who knows more about metallurgy might want to comment on the sensibility of this assessment.)
Is it possible that this particular filament lived its life so sedately that the end-of-life melting event was so gradual that it was confined to the core of the wire, rather than melting on the surface? (Perhaps some metallurgical effect also slightly raised the melting point of the surface.) In any case, from the visual evidence, it looks to me like this filament became liquid on the inside and then simply "broke" (note how there is really little or no gap between the severed ends). That *could* account for the hollow center. What it doesn't explain for me is where the molten metal went. It's an admittedly bizarre theory, but maybe it has some merit.
Anyway, thanks John for an intriguing puzzle.
Fred Schamber Aspex Corporation
-----Original Message----- X-from: johnf-at-geology.wisc.edu [mailto:johnf-at-geology.wisc.edu] Sent: Wednesday, September 05, 2007 1:33 PM To: Fred Schamber
I have found that when I image a recently failed W filament, it appears hollow where it failed (these are gently used filaments, with months and months of service). I have placed 6 images on a web page for you to view if interested. I would be interested in comments how this (hollowing) phenomenon occurs.
www.geology.wisc.edu/~johnf/filament.html
Thanks
John F -- ======================================================== John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480 Cameron Electron Microprobe Lab lab: (608) 265-4798 Dept of Geology & Geophysics fax: (608) 262-0693 University of Wisconsin home: (608) 274-2245 1215 West Dayton St. email: johnf-at-geology.wisc.edu Madison, WI 53706 amateur radio: WA3BTA Personal http://www.geology.wisc.edu/~johnf/ Probe lab http://www.geology.wisc.edu/~johnf/sx51.html Probe Sign Up Calender: http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi
"The first rule of all intelligent tinkering is to save every cog and wheel." -- Aldo Leopold
"For a successful technology, reality must take precedence over public relations, for Nature cannot be fooled." -- Richard P. Feynman
==============================Original Headers============================== 6, 24 -- From johnf-at-geology.wisc.edu Wed Sep 5 13:27:04 2007 6, 24 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l85IR2GX022876 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 13:27:04 -0500 6, 24 -- Received: from localhost (localhost [127.0.0.1]) 6, 24 -- by localhost (Postfix) with ESMTP id 26A2820D04 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 13:27:02 -0500 (CDT) 6, 24 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) 6, 24 -- by localhost (geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 6, 24 -- with ESMTP id 25784-05 for {microscopy-at-microscopy.com} ; 6, 24 -- Wed, 5 Sep 2007 13:26:53 -0500 (CDT) 6, 24 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57]) 6, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 6, 24 -- (No client certificate requested) 6, 24 -- by ice.geology.wisc.edu (Postfix) with ESMTP id 8D75720D06 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 5 Sep 2007 13:26:53 -0500 (CDT) 6, 24 -- Mime-Version: 1.0 6, 24 -- Message-Id: {p06230912c304a5223979-at-[144.92.206.57]} 6, 24 -- Date: Wed, 5 Sep 2007 13:26:50 -0500 6, 24 -- To: microscopy-at-microscopy.com 6, 24 -- From: John Fournelle {johnf-at-geology.wisc.edu} 6, 24 -- Subject: Images of worn thru W filament 6, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 6, 24 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu ==============================End of - Headers==============================
==============================Original Headers============================== 22, 20 -- From fschamber-at-aspexcorp.com Thu Sep 6 09:59:56 2007 22, 20 -- Received: from aspexcorp.com (mail.aspexcorp.com [67.141.199.81]) 22, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l86ExtiG025063 22, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Sep 2007 09:59:55 -0500 22, 20 -- Content-class: urn:content-classes:message 22, 20 -- Subject: RE: [Microscopy] Images of worn thru W filament 22, 20 -- MIME-Version: 1.0 22, 20 -- Content-Type: text/plain; 22, 20 -- charset="us-ascii" 22, 20 -- Date: Thu, 6 Sep 2007 10:59:50 -0400 22, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 22, 20 -- Message-ID: {65657329C810FE4B96B17390F9BE463E06C087-at-ASPEXCORP2K3.aspexcorp2k6.local} 22, 20 -- X-MS-Has-Attach: 22, 20 -- X-MS-TNEF-Correlator: 22, 20 -- Thread-Topic: [Microscopy] Images of worn thru W filament 22, 20 -- thread-index: Acfv6yhOAGFIEIOBS3KNf7xpziKStwAnaY0g 22, 20 -- From: "Fred Schamber" {fschamber-at-aspexcorp.com} 22, 20 -- To: {johnf-at-geology.wisc.edu} , {Microscopy-at-microscopy.com} 22, 20 -- Content-Transfer-Encoding: 8bit 22, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l86ExtiG025063 ==============================End of - Headers==============================
Thanks for all the suggestions for dewaxing leaf surfaces for SEM. The student now has several things to try!
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
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Email: dparmiter-at-ncifcrf.gov Name: David Parmiter
Organization: Nanotechnology Characterization Lab
Title-Subject: [Filtered] EM tissue processor opinions
Question: Hello all -
My organization is looking to purchase an EM tissue processor with a resin polymerizer, and I'd like to know if anyone works with or has worked with these instruments and has any opinions they would care to share about the efficiency, ease of use, and quality of these machines.
I have reviewed what Fred said and your images. I haven't received a posting from the list server for two months. Then I got this one. I called Fred and he said it came from the list server, not him.
In the last image you have a fractured area on the right side of the left part of the filament. On the right filament and in the back is the melting point of the failure. This is a strange type of failure. I have never seen it before.
At the end of a filament life, the grain boundaries increase in resistance and material is evaporated from those grain boundary areas first. That explains why you see the crystalline features in the images that look like etchings. The low mag shot shows that the filament was thinned on the sides like a normal filament that is not raised to extreme temperatures during initial use. I believe you ran this filament at reduced emission (increased self-bias resistance) but I could be wrong.
Near the end of life, the filament undergoes an exponential increase in heat dissipation at the future point of failure. That occurs at a lower current than is normally seen during normal filament usage. Why? The total resistance is increasing from thinning. How can it use less current, have less heat dissipation (I²Rt) and still melt the filament? I wrote an article about the failure of filaments, why they fail on the sides, modeled the failure mathematically, and what actually happens electronically during the failure and even what could happen after the failure. MT did not publish this article. I could post it but there are three figures that can't be posted to the list server.
At the end of life, the heat dissipation SHIFTS from the other areas of normal evaporation rates to the future point of failure. So even with less power supplied, the resistance locally at the point of failure is increased from thinning and that together with the increased heat dissipation melts the metal at that point. Fred is right. The resistance does increase but why doesn't the decrease in current prevent failure? The heat dissipation at the point of NO failure actually drops during the exponential failure. At the point of failure, heat dissipation increases exponentially and if it could continue, it would reach a peak of heat dissipation. That never happens. This is clearly seen in the images and figures I created but it was not published.
Your failure does seem to be bizarre. Somehow you evaporated the metal at the point of failure at a very slow rate, IMHO.
This is suggested by your fracture pattern on the left segment by the small cross section of fracture. The right segment does show some melting. So the power supplied did cause a critical failure even at reduced currents and small equivalent diameters. The diameter of the failure is very small indeed. So my speculation is that you ran on the false peak (or valley point), at a much lower self-bias setting, and/or at under saturation on the final rise to full saturation. The analysis of the false peak and its cause is complicated and takes more than 10,000 words to explain. Filament failure is bad enough.
So where were you operating and how? K. Heinrich clearly showed the overlap of sample or target currents with emission current and filament current in his diagrams about microprobes in his book in 1981. Look at how the plateau position varies with self-bias and the temperature of the filament plotted as voltage applied to the filament for heating. The X-axis' all have the same scale for all the plots. (Notice the TWO false peaks. Did the sides of the filament heat up twice? No, that side filament emission theory is not exactly correct.) I suspect that you operated at low emission currents and that allowed a VERY slow evaporation and thinning of the filament. I did this in a TEM and would get 6+ months per filament but I used an image analysis computer to boost up the brightness enough to operate for months on one filament. I stayed way to the left of the plateaus shown in Heinrich. I was TEM viewing screen saturated at lower self-bias but the temp was way lower.
I had two Heinrich page links corrected to show the proper self-bias ratings. I guess I'll use this one. http://www4.nau.edu/microanalysis/Microprobe/Column-ElectronGun.html
Notice how the start of the saturation plateau shifts with bias along the X-axis. Now notice that the scale of the X-axis is really filament temperature. Now notice that the emission current is a Michael Schaf's "monotonic rise" (Dec 2003) and where the plateau is located for each bias setting. At the higher bias, the plateau is shifted to LOWER temperatures. This is why I feel that your slower rate of tungsten evaporation caused the strange failure you saw in your images and that you used a really low evaporation rate by one of two or three routes.
Try this. Look at the lower sample saturation plateau. Now go up to the proper emission curve. Look at where you are on the emission plateau of the filament. Now do that for the lower bias. You are way up on the saturation plateau of the emission currents now. Now repeat this and look at the heating temperatures. Heinrich is clear. The use of the saturation plateau of the specimen (target) current is not very accurate in determining where you are on the emission (meter) plateau. In his figures, it is clear that a lower bias raises the temperature of the filament at "saturation" on the X-axis. At saturation on the specimen or sample, you are way further up than you think you are on the emission curve. Further increases on the X-axis beyond the start of the emission plateau, only evaporates more tungsten. The reason for this increased temp in practical use is to shift the plane of a net force of zero acceleration field for electrons closer to the tip of the filament, AKA the poorly named Zero Equipotential (ZEP).
Filament wire is pulled or drawn out of a die. If you had a defective cavity in the wire, then the wire should have failed during this process. It is not impossible for a cavity to form and survive but is highly improbable, IMO.
Another possibility is that you over heated the filament initially but quickly turned the filament down. That might have damaged the grain in the hottest part of the filament behind the tip but on the sides. This should cause an increase in resistance and a higher rate of evaporation at that point. If the grain boundaries "hollowed out" first, then maybe the interior grain evaporated from the increased heat dissipation seen but you operated at a lower emission current. So you still saw a decent filament life. But evaporative thinning will get you in the end based on operating temps of tungsten.
JMO based on my studies and my article on the "Heating Effects of Tungsten Wire" (and Nichrome wire).
I may not see any replies unless you include my email address. It could be a list server problem but it could also be my ISP's spam "cleaning" software.
HTH,
Paul Beauregard Senior Research Associate, emeritus Greensburg, PA
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==============================Original Headers============================== 20, 29 -- From beaurega-at-westol.com Thu Sep 6 21:14:42 2007 20, 29 -- Received: from smtp-gateway-3.winbeam.com (smtp-gateway-3.winbeam.com [64.84.97.68]) 20, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l872EfQb010706 20, 29 -- for {microscopy-at-microscopy.com} ; Thu, 6 Sep 2007 21:14:41 -0500 20, 29 -- X-Winbeam-MailScanner-Watermark: 1189736058.09953-at-4ylBYl63XRViT7fJVu/eIg 20, 29 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 20, 29 -- by smtp-gateway-3.winbeam.com (8.13.1/8.12.8) with SMTP id l872E8KM025067 20, 29 -- for {microscopy-at-microscopy.com} ; Thu, 6 Sep 2007 22:14:09 -0400 20, 29 -- Received: (qmail 9503 invoked by uid 89); 7 Sep 2007 02:14:02 -0000 20, 29 -- Received: from pitts-69-72-117-19.dynamic-dialup.coretel.net (HELO beaurega) (69.72.117.19) 20, 29 -- by mail.winbeam.com with SMTP; 7 Sep 2007 02:14:02 -0000 20, 29 -- Message-Id: {3.0.6.32.20070906221403.007ceaa0-at-pop3.norton.antivirus} 20, 29 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus (Unverified) 20, 29 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 20, 29 -- Date: Thu, 06 Sep 2007 22:14:03 -0400 20, 29 -- To: fschamber-at-aspexcorp.com, microscopy-at-microscopy.com, 20, 29 -- Philip Oshel {oshel1pe-at-cmich.edu} 20, 29 -- From: Beaurega {beaurega-at-westol.com} 20, 29 -- Subject: Re: [Microscopy] RE: Images of worn thru W filament 20, 29 -- In-Reply-To: {200709061500.l86F0Yxd025776-at-ns.microscopy.com} 20, 29 -- Mime-Version: 1.0 20, 29 -- Content-Type: text/plain; charset="iso-8859-1" 20, 29 -- Content-Transfer-Encoding: 8bit 20, 29 -- X--MailScanner-Information: - Please contact Technical Support for more information 20, 29 -- X--MailScanner: Found to be clean (courtesy of MailScanner) 20, 29 -- X--MailScanner-SpamCheck: not spam (whitelisted), SpamAssassin (not cached, 20, 29 -- score=-0.255, required 4, autolearn=not spam, AWL 2.75, 20, 29 -- BAYES_50 2.00, local_FROM_WB -1.00, local_HAM_FROM_WB -4.00) 20, 29 -- X--MailScanner-From: beaurega-at-westol.com ==============================End of - Headers==============================
I think I'm missing something basic here, but it's 4:00 in the morning and I can't figure this one out. I'm trying to get my SEM vacuum system's piranni gauge sensors calibrated (They were way off, allowing the HV to switch on at just a little below 1 atm!). I'm using a Penning 8 controller with a Penning model CP25-K sensor head. Both are manufactured by Edwards.
The scale reading on the gauge starts at 10E-5 on the left side (Using scale 1) and goes to 10E-2 on the right hand side. I plugged the sensor head directly on my vacuum pump to see if the meter works, and as the vacuum increases, the needle goes from left to right, or, according to the labeling behind the needle, from .00001 torr (As it is read on the meter, even though the sensor is at 1 atm) and eventually settled on .001 torr, and doesn't move. Now, I'm not getting any movement on the piranni gauges either, so the vacuum is not changing.
When I had the pump connected directly to the meter, the needle went from left to right. I switched scales to scale 2, and the needle again went from left to right.
So, my confusion is why does the labeling on the gauge go from high vacuum to low vacuum as you go left to right, and the needle goes from low vacuum to high vacuum as you go from left to right. It seems backwards.
Granted, there is probably something stupid I'm missing...
Dear Renu, I haven't seen any other responses to your question, so...
I'm assuming you're using TEM and that the moire fringes you're seeing arise when you have two (or more) sheets of overlapping material. In which case they will be 'rotation' moire fringes due to differences in the orientation of the crystal planes in the two sheets; and possibly 'dilatation' moire fringes also if you are exciting different diffraction conditions in the two plates (e.g. 111 and 110 planes happen to be close to parallel and they're both diffracting). IF you can work out which diffraction condition holds in each of the two plates you can work out the orientation relationship between them very accurately using the moire fringe spacing; they will also show up any dislocations in the material very nicely. But I can't think of any way to get sample thickness from them. Probably the easiest way to get thickness is to identify features on the surfaces of the platelets and use geometry. Take some stereo pairs (which are fun to do anyway!)
Justin, A Penning gauge will often strike at pressures higher than it can read. At those higher pressures, the conductivity of the gases is quite low which is why you get the better vacuum readings which then appear to degrade as the vacuum actually gets better and ionization improves, coming into a logarithmic range that gives accurate readings.
If you are going to test the RP, use either a pirani gauge or a thermocouple gauge. Penning gauges are designed for lower pressures than a questionable RP can produce. Once you can determine that there is, in fact, a high vacuum (above the DP), the Penning gauge can be used to set the zero point for the pirani or TC gauge in the high vacuum area, but using it to try and read a rough vacuum is not a good idea. Pirani or TC gauges in areas that never see a high vacuum can then be calibrated to the gauge (or gauges) that do see high vac and have been calibrated from atmosphere to "0", usually somewhere around 10E-3 to 10E-4 Torr (1.0 to 0.1 microns). If "0" is set at 10E-5T or better, this can be considered calibrated.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Friday, September 07, 2007 4:02 AM To: kenconverse-at-qualityimages.biz
I think I'm missing something basic here, but it's 4:00 in the morning and I can't figure this one out. I'm trying to get my SEM vacuum system's piranni gauge sensors calibrated (They were way off, allowing the HV to switch on at just a little below 1 atm!). I'm using a Penning 8 controller with a Penning model CP25-K sensor head. Both are manufactured by Edwards.
The scale reading on the gauge starts at 10E-5 on the left side (Using scale 1) and goes to 10E-2 on the right hand side. I plugged the sensor head directly on my vacuum pump to see if the meter works, and as the vacuum increases, the needle goes from left to right, or, according to the labeling behind the needle, from .00001 torr (As it is read on the meter, even though the sensor is at 1 atm) and eventually settled on .001 torr, and doesn't move. Now, I'm not getting any movement on the piranni gauges either, so the vacuum is not changing.
When I had the pump connected directly to the meter, the needle went from left to right. I switched scales to scale 2, and the needle again went from left to right.
So, my confusion is why does the labeling on the gauge go from high vacuum to low vacuum as you go left to right, and the needle goes from low vacuum to high vacuum as you go from left to right. It seems backwards.
Granted, there is probably something stupid I'm missing...
Maybe I can help. The key is that {for the intended range of vacuum} the Penning gauge current decreases with decreasing PRESSURE - there are less molecules to ionize and cause a current. However, it is not linear over the whole pressure range, and typically becomes nonlinear at something like 2x10-4 torr and at sufficiently high pressure the discharge extinguishes completely (like a sputter coater at too high a pressure) and the gauge will indicate to the left, like a very good vacuum, but basically VERY poor vacuum. So I think this is why the vacuum may seem to get worse (left to right meter movement) initially - then probably falls (?).
Pirani gages are often used to enable the discharge gage only at about 1x10-2 torr so that you don't get a "false minimum" indication. The Kinney discharge gage is representative of general discharge (Penning) performance.
And pirani gauges themselves are not very sensitive right up to atmosphere - you have probably noticed that there is a lag in the deflection as the rough pump is initially switched on.
(I separately sent Justin attachements that can't go to the list - Excel and OpenOffice versions of spreadsheets that have some Discharge Gage and Pirani gage info I had compiled for a re-work of a Kinney Vacuum Discharge/Thermocouple gage set. I can post this on my webspace or email anyone interested....)
Dale
kraftpiano-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I think I'm missing something basic here, but it's 4:00 in the morning } and I can't figure this one out. I'm trying to get my SEM vacuum } system's piranni gauge sensors calibrated (They were way off, allowing } the HV to switch on at just a little below 1 atm!). I'm using a } Penning 8 controller with a Penning model CP25-K sensor head. Both } are manufactured by Edwards. } } The scale reading on the gauge starts at 10E-5 on the left side (Using } scale 1) and goes to 10E-2 on the right hand side. I plugged the } sensor head directly on my vacuum pump to see if the meter works, and } as the vacuum increases, the needle goes from left to right, or, } according to the labeling behind the needle, from .00001 torr (As it } is read on the meter, even though the sensor is at 1 atm) and } eventually settled on .001 torr, and doesn't move. Now, I'm not } getting any movement on the piranni gauges either, so the vacuum is } not changing. } } When I had the pump connected directly to the meter, the needle went } from left to right. I switched scales to scale 2, and the needle } again went from left to right. } } So, my confusion is why does the labeling on the gauge go from high } vacuum to low vacuum as you go left to right, and the needle goes from } low vacuum to high vacuum as you go from left to right. It seems } backwards. } } Granted, there is probably something stupid I'm missing... } } --Justin A. Kraft } } ==============================Original Headers============================== } 6, 27 -- From kraftpiano-at-gmail.com Fri Sep 7 02:59:01 2007 } 6, 27 -- Received: from nz-out-0506.google.com (nz-out-0506.google.com [64.233.162.236]) } 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l877x0eV028612 } 6, 27 -- for {microscopy-at-microscopy.com} ; Fri, 7 Sep 2007 02:59:01 -0500 } 6, 27 -- Received: by nz-out-0506.google.com with SMTP id o37so288169nzf } 6, 27 -- for {microscopy-at-microscopy.com} ; Fri, 07 Sep 2007 00:59:00 -0700 (PDT) } 6, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 27 -- d=gmail.com; s=beta; } 6, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 6, 27 -- bh=SxvpmzcgH9e6/2OtuoHLuFvZsM69Vy1lKSk2bzNhKWw=; } 6, 27 -- b=Y60U5HoCib8ITXUFcxCDf64DCAyTKqP0a+rnDS8TFg1b8P2gdVMKTPZHGoub+F48N3hmcV9UpAOXVPW0TNkoykOH6xWkekdmVJ+Ou5dXZF+nOeqeuYgt+Z9OOropq+Et/wszoaIP8kg3BPgUGP3Zy46tRVLuKulnuTQ7PfCrabg= } 6, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 6, 27 -- d=gmail.com; s=beta; } 6, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 6, 27 -- b=Yisi6RiuDMGxg0RAehINCdIBjwQrVuia8byhVPP4PAk7b/om95YdHbR8SrA8ihi53qCk/AM8S1FIo6n6BBgZvet3Tsl8Mxj+JIq863+1a8BrzB/Dsrpsun8mnGPJXIO3RXB+ahp37XzUlgX1Ihg6COtzidRxbTpfw/Z+4xWFRLU= } 6, 27 -- Received: by 10.142.222.21 with SMTP id u21mr75370wfg.1189151940146; } 6, 27 -- Fri, 07 Sep 2007 00:59:00 -0700 (PDT) } 6, 27 -- Received: by 10.143.12.14 with HTTP; Fri, 7 Sep 2007 00:59:00 -0700 (PDT) } 6, 27 -- Message-ID: {25e2b0d20709070059o19134366i1732e5020f47f9e9-at-mail.gmail.com} } 6, 27 -- Date: Fri, 7 Sep 2007 03:59:00 -0400 } 6, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} } 6, 27 -- To: microscopy-at-microscopy.com } 6, 27 -- Subject: Penning gauge confusion. } 6, 27 -- MIME-Version: 1.0 } 6, 27 -- Content-Type: text/plain; charset=ISO-8859-1 } 6, 27 -- Content-Transfer-Encoding: 7bit } 6, 27 -- Content-Disposition: inline } ==============================End of - Headers==============================
-- } {(((º} L L } {(((º} .·´¯`·.¸ {º)))} { ¸.·´¯`·.¸ } {(((º} ¸.·´¯¯¯¯¯¯¯
Dale A. Callaham Central Microscopy Facility The University of Massachusetts Amherst, MA 01003
At the risk of wasting bandwidth, it seems to me that the essential part of this discussion really concerns the mechanism of action of a Penning gauge. That is, that there is a high voltage applied between a central electrode and ground (generally the wall of the tube). The central electrode is mounted at a distance from wall of the tube, and insulated from it. The gauge then measures the current between these points. Current can only flow when there are enough ions between the electrodes. Current will not flow when there are too few ions, such as at a high vacuum, or when the flow of ions is impeded by lots of other uncharged molecules, at low vacuum. Thus, as others have said, if one starts following a Penning gauge shortly after beginning a vacuum run, it will start reading low current. As the vacuum improves, the current will increase (due to more ions), but then, as the vacuum becomes even better, the current will then decrease again.
Joel
Date sent: Fri, 7 Sep 2007 09:59:32 -0500 To: jbs-at-temple.edu X-from: dac-at-research.umass.edu Send reply to: dac-at-research.umass.edu
One other thing is that the design of most discharge gage meter units supplies something like 2200-2700V to the central electrode, but always this should have a limiting resistor (1M ohm is typical) to prevent electrocutions and limit power dissipation - it also reduces the actual voltage at the gage dependent on current (IR drop across the resistor) and this more severe foldback is part of the non-linear response typical of the discharge gage units that is not an intrinsic part of Penning discharge characteristics due only to higher pressue.
Dale
Joel Sheffield wrote: } At the risk of wasting bandwidth, it seems to me that the essential } part of this discussion really concerns the mechanism of action of a } Penning gauge. That is, that there is a high voltage applied between } a central electrode and ground (generally the wall of the tube). The } central electrode is mounted at a distance from wall of the tube, and } insulated from it. The gauge then measures the current between these } points. Current can only flow when there are enough ions between the } electrodes. Current will not flow when there are too few ions, such } as at a high vacuum, or when the flow of ions is impeded by lots of } other uncharged molecules, at low vacuum. Thus, as others have said, } if one starts following a Penning gauge shortly after beginning a } vacuum run, it will start reading low current. As the vacuum } improves, the current will increase (due to more ions), but then, as } the vacuum becomes even better, the current will then decrease again. } } Joel } } } Date sent: Fri, 7 Sep 2007 09:59:32 -0500 } To: jbs-at-temple.edu } From: dac-at-research.umass.edu } Send reply to: dac-at-research.umass.edu } Subject: [Microscopy] Re: Penning gauge confusion. } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi Justin, } } } } Maybe I can help. The key is that {for the intended range of vacuum} the } } Penning gauge current decreases with decreasing PRESSURE - there are } } less molecules to ionize and cause a current. However, it is not linear } } over the whole pressure range, and typically becomes nonlinear at } } something like 2x10-4 torr and at sufficiently high pressure the } } discharge extinguishes completely (like a sputter coater at too high a } } pressure) and the gauge will indicate to the left, like a very good } } vacuum, but basically VERY poor vacuum. So I think this is why the } } vacuum may seem to get worse (left to right meter movement) initially - } } then probably falls (?). } } } } Pirani gages are often used to enable the discharge gage only at about } } 1x10-2 torr so that you don't get a "false minimum" indication. The } } Kinney discharge gage is representative of general discharge (Penning) } } performance. } } } } } Kinney Discharge Gage NOTE the non-linear response at } 2x10-4 mTorr } } } } } } mTorr Torr Microamps } } } 10.0000 1.00E-02 1800 } } } 2.0000 2.00E-03 1700 } } } 1.0000 1.00E-03 1600 } } } 5.00E-04 1500 } } } 3.00E-04 1300 } } } 2.00E-04 1000 } } } 0.1000 1.00E-04 500 } } } 0.0100 1.00E-05 50 } } } 0.0010 1.00E-06 5 } } } 0.0001 1.00E-07 0.5 } } } } } } And pirani gauges themselves are not very sensitive right up to } } atmosphere - you have probably noticed that there is a lag in the } } deflection as the rough pump is initially switched on. } } } } (I separately sent Justin attachements that can't go to the list - Excel } } and OpenOffice versions of spreadsheets that have some Discharge Gage } } and Pirani gage info I had compiled for a re-work of a Kinney Vacuum } } Discharge/Thermocouple gage set. I can post this on my webspace or email } } anyone interested....) } } } } Dale } } } } } } kraftpiano-at-gmail.com wrote: } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } I think I'm missing something basic here, but it's 4:00 in the morning } } } and I can't figure this one out. I'm trying to get my SEM vacuum } } } system's piranni gauge sensors calibrated (They were way off, allowing } } } the HV to switch on at just a little below 1 atm!). I'm using a } } } Penning 8 controller with a Penning model CP25-K sensor head. Both } } } are manufactured by Edwards. } } } } } } The scale reading on the gauge starts at 10E-5 on the left side (Using } } } scale 1) and goes to 10E-2 on the right hand side. I plugged the } } } sensor head directly on my vacuum pump to see if the meter works, and } } } as the vacuum increases, the needle goes from left to right, or, } } } according to the labeling behind the needle, from .00001 torr (As it } } } is read on the meter, even though the sensor is at 1 atm) and } } } eventually settled on .001 torr, and doesn't move. Now, I'm not } } } getting any movement on the piranni gauges either, so the vacuum is } } } not changing. } } } } } } When I had the pump connected directly to the meter, the needle went } } } from left to right. I switched scales to scale 2, and the needle } } } again went from left to right. } } } } } } So, my confusion is why does the labeling on the gauge go from high } } } vacuum to low vacuum as you go left to right, and the needle goes from } } } low vacuum to high vacuum as you go from left to right. It seems } } } backwards. } } } } } } Granted, there is probably something stupid I'm missing... } } } } } } --Justin A. Kraft } } } } } } ==============================Original Headers============================== } } } 6, 27 -- From kraftpiano-at-gmail.com Fri Sep 7 02:59:01 2007 } } } 6, 27 -- Received: from nz-out-0506.google.com (nz-out-0506.google.com [64.233.162.236]) } } } 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l877x0eV028612 } } } 6, 27 -- for {microscopy-at-microscopy.com} ; Fri, 7 Sep 2007 02:59:01 -0500 } } } 6, 27 -- Received: by nz-out-0506.google.com with SMTP id o37so288169nzf } } } 6, 27 -- for {microscopy-at-microscopy.com} ; Fri, 07 Sep 2007 00:59:00 -0700 (PDT) } } } 6, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } } 6, 27 -- d=gmail.com; s=beta; } } } 6, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } } } 6, 27 -- bh=SxvpmzcgH9e6/2OtuoHLuFvZsM69Vy1lKSk2bzNhKWw=; } } } 6, 27 -- b=Y60U5HoCib8ITXUFcxCDf64DCAyTKqP0a+rnDS8TFg1b8P2gdVMKTPZHGoub+F48N3hmcV9UpAOXVPW0TNkoykOH6xWkekdmVJ+Ou5dXZF+nOeqeuYgt+Z9OOropq+Et/wszoaIP8kg3BPgUGP3Zy46tRVLuKulnuTQ7PfCrabg= } } } 6, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } } } 6, 27 -- d=gmail.com; s=beta; } } } 6, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } } } 6, 27 -- b=Yisi6RiuDMGxg0RAehINCdIBjwQrVuia8byhVPP4PAk7b/om95YdHbR8SrA8ihi53qCk/AM8S1FIo6n6BBgZvet3Tsl8Mxj+JIq863+1a8BrzB/Dsrpsun8mnGPJXIO3RXB+ahp37XzUlgX1Ihg6COtzidRxbTpfw/Z+4xWFRLU= } } } 6, 27 -- Received: by 10.142.222.21 with SMTP id u21mr75370wfg.1189151940146; } } } 6, 27 -- Fri, 07 Sep 2007 00:59:00 -0700 (PDT) } } } 6, 27 -- Received: by 10.143.12.14 with HTTP; Fri, 7 Sep 2007 00:59:00 -0700 (PDT) } } } 6, 27 -- Message-ID: {25e2b0d20709070059o19134366i1732e5020f47f9e9-at-mail.gmail.com} } } } 6, 27 -- Date: Fri, 7 Sep 2007 03:59:00 -0400 } } } 6, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} } } } 6, 27 -- To: microscopy-at-microscopy.com } } } 6, 27 -- Subject: Penning gauge confusion. } } } 6, 27 -- MIME-Version: 1.0 } } } 6, 27 -- Content-Type: text/plain; charset=ISO-8859-1 } } } 6, 27 -- Content-Transfer-Encoding: 7bit } } } 6, 27 -- Content-Disposition: inline } } } ==============================End of - Headers============================== } } -- } } } {(((º} } } L L } } } {(((º} .·´¯`·.¸ {º)))} { ¸.·´¯`·.¸ } {(((º} ¸.·´¯¯¯¯¯¯¯ } } } } Dale A. Callaham } } Central Microscopy Facility } } The University of Massachusetts } } Amherst, MA 01003 } } } } } } ==============================Original Headers============================== } } 13, 22 -- From dac-at-research.umass.edu Fri Sep 7 09:59:20 2007 } } 13, 22 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) } } 13, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l87ExKl4008930 } } 13, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Sep 2007 09:59:20 -0500 } } 13, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) } } 13, 22 -- (authenticated bits=0) } } 13, 22 -- by race2.oit.umass.edu (8.13.7/8.13.7) with ESMTP id l87ExJEM027448 } } 13, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } } 13, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Sep 2007 10:59:19 -0400 } } 13, 22 -- Message-ID: {46E1766D.6070508-at-research.umass.edu} } } 13, 22 -- Date: Fri, 07 Sep 2007 11:03:57 -0500 } } 13, 22 -- From: Dale Callaham {dac-at-research.umass.edu} } } 13, 22 -- Reply-To: dac-at-research.umass.edu } } 13, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.6) Gecko/20070802 SeaMonkey/1.1.4 } } 13, 22 -- MIME-Version: 1.0 } } 13, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} } } 13, 22 -- Subject: Re: [Microscopy] Penning gauge confusion. } } 13, 22 -- References: {200709070806.l87861tg005386-at-ns.microscopy.com} } } 13, 22 -- In-Reply-To: {200709070806.l87861tg005386-at-ns.microscopy.com} } } 13, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 13, 22 -- Content-Transfer-Encoding: 8bit } } 13, 22 -- X-Whitelist: TRUE } } ==============================End of - Headers============================== } } } -- } Joel B. Sheffield, Ph.D. } Biology Department, Temple University } 1900 North 12th Street } Philadelphia, PA 19122 } jbs-at-temple.edu } (215) 204 8839, fax (215) 204 0486 } http://astro.temple.edu/~jbs }
-- } {(((º} L L } {(((º} .·´¯`·.¸ {º)))} { ¸.·´¯`·.¸ } {(((º} ¸.·´¯¯¯¯¯¯¯
Dale A. Callaham Central Microscopy Facility The University of Massachusetts Amherst, MA 01003
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Title-Subject: [Filtered] Used Water Chiller Wanted
Question: I want to kmow if anyone out there have a used water chiller unit for sale? The unit is for a Zeiss 902 TEM.
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Email: hoelyas-at-yahoo.com Name: Hossein
Organization: laboratory
Title-Subject: [Filtered] EM900 service
Question: Hi, all I have a Zeiss EM900 TEM,when switch it on the "water check" lamp glows, our serviceman needs the service manual our at least it's schematic diagram,to repair it,would someone help me please? Best Regard ,H.Elyas hoelyas-at-yahoo.com
Here is the September 2007 Microscopy Today table of contents. I will close the subscription list for this issue on Thursday September 13th, 2007.
Microscopists in North America and MSA members anywhere qualify for free subscriptions. Anyone else may subscribe for US$50 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com . Subscription rate for non-qualified readers will go to US$60 in 2008.
Thank you.
Ron Anderson, Editor ================================ Directing Traffic in Lymph Nodes Stephen W. Carmichael and Ellen D. Remstein, Mayo Clinic, Rochester, MN
Multi-Length Scale Characterization of the Gibeon Meteorite using Electron Backscatter Diffraction Matthew M. Nowell and John O. Carpenter, EDAX-TSL, Draper UT
SEM Provides Critical Process Information in Pharmaceutical Applications Ben Lich, FEI Company, Hillsboro, OR
Optimal Noise Filters in High-Resolution Electron Microscopy K. Ishizuka, P. H. C. Eilers* and T. Kogure**, HREM Research Inc., Higashimatsuyama, Japan, *Utrecht University, Utrecht, The Netherlands, **University of Tokyo, Tokyo, Japan
Quantification of Contaminant Removal by Evactron Cleaning Using Quartz Crystal Thickness Monitors Christopher G. Morgan, Mark M. Gleason and Ronald Vane, XEI Scientific, Inc., Redwood City, CA
Reconstructing What Was: Software Applied to Serial Section TEM Marcia D. Feinberg and John C. Fiala, Boston University, Boston MA
Overcoming Challenges in Material Science Testing with the use of Large Specimen SEM Analysis Adriana Romero, VisiTec of America LLC, Knoxville, TN
Microscopic analysis of magmatic crystals ? Part 2: A SEM study of the stability of accessory zircon under increasing metamorphic conditions Robert Sturm, Department of Materials Engineering and Physics, University of Salzburg/Austria
Specimen Preparation for SEM examination of Thin Polymer Films Gan Phay Fang, Science & Technology Innovative Centre, Ansell Shah Alam Sdn Bhd, Selangor, Malaysia
Applications of Focused Ion Beam (FIB) on Yeast Cell & SARS Virus H. L. Hing1, C. Burkhardt2, P. Gnauck2, S. Sally3, H. Gelderbloms4, Y. Muranaka5, M.A. Kaswandi1, A.H. A. Aziz1 & A.Z. Sahalan1. 1National University of Malaysia, Kuala Lumpur, 2(NMI), Reutlingen, Germany, 3National University, Canberra, Australia, 4Robert Koch Institut, Berlin, Germany, 5 Hamamatsu University School of Medicine, Hamamatsu, Japan
Advanced Metallographic Techniques Applied to Diesel Particulate Filters Natalio Saenz, Heather Dillon, Shelley Carlson, & Gary Maupin, Battelle PNNL, Richland, WA
New Approaches to Managing, Marketing, and Money for Maintaining a Core Facility (4Ms) Part 3: Marketing and Managing a Research Core/Facility Pankaj Sharma, Purdue University
The Beginnings of the Southeastern Microscopy Society W. Gray (Jay) Jerome, SEMS Historian
Industry News
NetNotes SPECIMEN PREPARATION - colloidal gold conjugation of proteins SPECIMEN PREPARATION ? paraffin dewaxing SPECIMEN PREPARTION ? measuring resin components SPECIMEN PREPARATION ? fixation of low pH extremophiles SPECIMEN PREPARATION ? UV polymerization SPECIMEN PREPARATION - SEM glue without carbon TEM ? calibration TEM - 120 Kev vs 200 Kev instruments TEM ? cause of specimen damage TEM & SEM terminology - kV or keV? ELECTRON MICROPROBE - carbon coater SEM/EDX - thin film thickness measurement**
Advertiser's Index
==============================Original Headers============================== 25, 17 -- From randerson20-at-tampabay.rr.com Sun Sep 9 10:23:48 2007 25, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.122]) 25, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l89FNlxV019793 25, 17 -- for {Microscopy-at-Microscopy.Com} ; Sun, 9 Sep 2007 10:23:47 -0500 25, 17 -- Received: from [127.0.0.1] (really [24.73.73.214]) 25, 17 -- by hrndva-omta01.mail.rr.com with ESMTP 25, 17 -- id {20070909152347.WDRS4050.hrndva-omta01.mail.rr.com-at-[127.0.0.1]} 25, 17 -- for {Microscopy-at-Microscopy.Com} ; Sun, 9 Sep 2007 15:23:47 +0000 25, 17 -- Message-ID: {46E41000.6040205-at-tampabay.rr.com} 25, 17 -- Date: Sun, 09 Sep 2007 11:23:44 -0400 25, 17 -- From: Ronald Anderson {randerson20-at-tampabay.rr.com} 25, 17 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 25, 17 -- MIME-Version: 1.0 25, 17 -- To: Listserver {Microscopy-at-Microscopy.Com} 25, 17 -- Subject: Microscopy Today September 2007 Table of Contents 25, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 25, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have a whole series of proceedings for Scanning electron microscopy starting in 1968 and going through the early 80's.
The first one is the proceedings of the Symposium on the Scanning Electron Microscope: the instrument and its applications held on April 30-May 1, 1968 in Chicago.
The others are every year through 1982.
They are free to anyone who will pay shipping. Otherwise they will be discarded.
Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 8, 21 -- From dsherman-at-purdue.edu Sun Sep 9 12:24:00 2007 8, 21 -- Received: from 1061exfe04a.itap.purdue.edu (1061exfe04a.itap.purdue.edu [128.210.1.11]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l89HO08p001743 8, 21 -- for {microscopy-at-microscopy.com} ; Sun, 9 Sep 2007 12:24:00 -0500 8, 21 -- Received: from exchange.purdue.edu ([172.21.6.23]) by 1061exfe04a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 21 -- Sun, 9 Sep 2007 13:24:00 -0400 8, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 8, 21 -- Sun, 9 Sep 2007 17:24:00 +0000 8, 21 -- User-Agent: Microsoft-Entourage/11.3.6.070618 8, 21 -- Date: Sun, 09 Sep 2007 13:23:58 -0400 8, 21 -- Subject: Historical scanning books 8, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 8, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 8, 21 -- Message-ID: {C309A46E.21B5B%dsherman-at-purdue.edu} 8, 21 -- Thread-Topic: Historical scanning books 8, 21 -- Thread-Index: AcfzBjSHc0xR2F75EdyWGQARJN08Mg== 8, 21 -- Mime-version: 1.0 8, 21 -- Content-type: text/plain; 8, 21 -- charset="US-ASCII" 8, 21 -- Content-transfer-encoding: 7bit 8, 21 -- X-OriginalArrivalTime: 09 Sep 2007 17:24:00.0310 (UTC) FILETIME=[35E80560:01C7F306] ==============================End of - Headers==============================
JEOL UK, as part of the expansion of its Electron Optics division, has a vacancy for a Sales Executive. The successful candidate will have a good knowledge of electron optical instrumentation and whilst sales experience is desirable, it is not essential.
JEOL UK has an experienced and successful sales team that the successful candidate should be able to fit in with and complement the existing sales people.
If you are interested in this position, please contact amys-at-jeoluk.com with your current CV.
PLEASE NOTE - Agressive SPAM filtering is employed. If you don't get a reply to e-mails, try again, avoiding anything in the subject or body which might trigger filtering. If you are in the address book, you should get through. If you aren't, then there's a chance your e-mail will never be seen.
==============================Original Headers============================== 5, 14 -- From larry-at-cymru666.plus.com Sun Sep 9 13:37:52 2007 5, 14 -- Received: from fhw-relay07.plus.net (fhw-relay07.plus.net [212.159.14.215] (may be forged)) 5, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l89IbqKd014831 5, 14 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sun, 9 Sep 2007 13:37:52 -0500 5, 14 -- Received: from [87.113.17.164] (helo=[192.168.1.2]) 5, 14 -- by fhw-relay07.plus.net with esmtp (Exim) id 1IURfK-0007VS-R0 5, 14 -- for Microscopy-at-MSA.Microscopy.Com; Sun, 09 Sep 2007 19:37:51 +0100 5, 14 -- Mime-Version: 1.0 5, 14 -- Message-Id: {p06240802c309ecd19b5b-at-[192.168.1.2]} 5, 14 -- Date: Sun, 9 Sep 2007 19:37:44 +0100 5, 14 -- To: Microscopy-at-MSA.Microscopy.Com 5, 14 -- From: Larry Stoter {larry-at-cymru666.plus.com} 5, 14 -- Subject: Vacancy with JEOL UK 5, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
In reaction to the question asked on the list, here is my opinion:
To my knowledge it is not uncommon to remove the substrate/membrane and re-embed with more resin to fill the gap. It justs takes some more time, but the technique is very easy. So if it works, why not?
You could grow your cells on porous PET membrane (from millipore or BD biosciences), those used for transport studies. They may stick better to the cells without damaging your knife (especially if they are coated with collagen, fibrillarin or whatever), and some resin (and even cell extensions) may enter the pores in the membrane, making the detachment of the membrane more unlikely. The disadvantage is that these membranes are very thin and flexible (and also pretty expensive, but you can do that in 96W format), which you can transform into an advantage: you can cut fine bands of membrane and for each band try different embedding/cutting conditions.
Now here is something a little bit crazy you could try : In the ultramicrotome, align you membrane horizontally (parallel to the knife edfge), cells down so that you cut your cells first. I did that on cells grown on PET membranes and it worked. Sometimes the sections breaks open between the cells and the membrane, but there is always some places where are they still one against the other. The section may be thicker close to the membrane, though.
Good luck!
Stephane
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Email: darkmatterfound-at-gmail.com Name: Victor Lo
Organization: ANSTO
Title-Subject: [Filtered] Staining samples
Question: Hello all,
I am wondering if anyone can provide me with some staining service for various forms of starch to look at the morphology and structure changes. The papers that have been found indicated that they use a periodic acid-thisemicabazide-silver reaction. If there is anyone that can do this in Australia please drop me a message.
Listers, We are recruiting. Please see the details below or go to the link.
Faculty Position in Cell Biology University of Texas Southwestern Medical Center at Dallas
The Department of Cell Biology at The University of Texas Southwestern Medical Center at Dallas seeks to appoint exceptional scientists who are experts in the fields of general cell biology, live cell imaging, cryoEM, or electron tomography to the position of Assistant Professor (tenure track). Candidates must have a Ph.D. or M.D. and be doing cutting edge research at the interface between cell and molecular biology in such areas as: organization of macromolecular complexes, molecular interactions in living cells, spatial organization of signal transduction and cellular basis of tissue organization. The excellence of the individual candidate will take precedence over the area of special interest. The successful candidate will join an internationally recognized Cell Biology faculty at a top rated medical institution and receive both a competitive salary and an exceptional start-up package. Women and minority candidates are encouraged to apply. For more information, visit the Cell Biology web site at http://www8.utsouthwestern.edu/utsw/cda/dept25128/files/34664.html Applicants should email their curriculum vitae, the names of three references, and a brief description of their research goals to the attention of Dr. Richard G. W. Anderson at cb.recruitment-at-utsouthwestern.edu The University of Texas Southwestern Medical Center is an Affirmative Action/Equal Opportunity Employer. Women and minority candidates are encouraged to apply.
Christopher J Gilpin Ph.D. Assistant Professor Director, Molecular and Cellular Imaging Facility K1.A04 UT Southwestern Medical Center at Dallas 5323 Harry Hines Boulevard Dallas, TX 75390-9039 Phone +1 214 648 2827 Fax +1 214 648 6408
==============================Original Headers============================== 10, 21 -- From christopher.gilpin-at-utsouthwestern.edu Mon Sep 10 09:14:19 2007 10, 21 -- Received: from swlx167.swmed.edu (swlx167.swmed.edu [199.165.152.167]) 10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8AEEISx009644 10, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Sep 2007 09:14:19 -0500 10, 21 -- Received: from [129.112.148.180] (helo=cgdesktop) 10, 21 -- by swlx167.swmed.edu with esmtp (Exim 4.44) 10, 21 -- id 1IUk1o-0006yF-Ui 10, 21 -- for Microscopy-at-microscopy.com; Mon, 10 Sep 2007 09:14:17 -0500 10, 21 -- From: "Christopher Gilpin" {christopher.gilpin-at-utsouthwestern.edu} 10, 21 -- To: {Microscopy-at-microscopy.com} 10, 21 -- Date: Mon, 10 Sep 2007 09:17:20 -0500 10, 21 -- Message-ID: {001601c7f3b5$4caa5550$b4947081-at-cgdesktop} 10, 21 -- MIME-Version: 1.0 10, 21 -- Content-Type: text/plain; 10, 21 -- charset="US-ASCII" 10, 21 -- Content-Transfer-Encoding: 7bit 10, 21 -- X-Mailer: Microsoft Office Outlook 11 10, 21 -- Thread-Index: AcfztUyUkXdCMea7ShSwAAqs3+y7SQ== 10, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 10, 21 -- X-Scan-Signature: 91c5be5f340ec53dc388915214e1bad9 10, 21 -- Subject: Open faculty position ==============================End of - Headers==============================
I use SEM, especially cryo-SEM to look at starch and I find it is good to show granule structure and morphology changes. Cryo-SEM does not require any sample preparation at all... But I am interested in knowing about that dying method and what structural features can be so observed.
Best regards
Antonio D. Molina-García
Instituto del Frío (CSIC) José Antonio Nováis, 10 Ciudad Universitaria 28040 Madrid Spain
----- Original Message ----- X-from: {darkmatterfound-at-gmail.com} To: {ifrm111-at-if.csic.es} Sent: Monday, September 10, 2007 3:17 PM
Hi Ralph,
We just surplused a scruffy, looking Haskris that came off our recently-replaced JEOL 1200EX. It has a relatively new compressor and other parts, but probably needs a new valve that shuts off water flow when the compressor isn't running. It has been working fine.
For information on this chiller, you can contact our Surplus folks. Info at: http://www.surplus.missouri.edu/. They picked it up last week, so it should still be available. It might even be up on eBay by now.
Good luck, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: rnichols-at-bcm.com [mailto:rnichols-at-bcm.com] Sent: Saturday, September 08, 2007 10:24 AM To: Tindall, Randy D.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both rnichols-at-bcm.com as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: rnichols-at-bcm.com Name: ralph nichols
Organization: Baylor College of Medicine
Title-Subject: [Filtered] Used Water Chiller Wanted
Question: I want to kmow if anyone out there have a used water chiller unit for sale? The unit is for a Zeiss 902 TEM.
I got the following helpful answer to my question and wanted to re-post the answer to the group.
Hi Emily, YES there is!!
It's called dispersion staining. You need a special objective with an annular stop and 1.544HD refractive index liquid. The HD stands for high dispersion. You can find instructions in McCrone/Delly's "Polarized Light Microscopy", but basically you establish a cylinder of light slightly smaller than your central stop. Particles with refractive indexes very close to the mounting media will show colored edges depending on the crystal orientation and polarizer orientation. The combination of color, refractive index produces unique characterization. Mind the temperature.
PS: if you have a polarizing filter on your phase contrast scope, you may be able to use a larger stop to get a dark field/dispersion staining effect.
I used to pre-screen clay for alpha quartz at Goodyear Tire and Rubber. If I couldn't find any (2 or 3 preps worth of sample) the sample was clean. If I detected quartz it went to X-ray diffraction I could detect quartz in lower amounts than X-ray diffraction.
I really like dispersion staining, its a great identity and confirmation tool!
I need some info to help a researcher who visited the lab today.
He wants to identify the elements, especially iron and phosphorus, in small, 5 um or so, plankton particles from the ocean.
He had a paper showing this done using a TEM and EDS system. I don't have an EDS system on our TEM, at least one that works, so I am fishing for ideas and assistance.
How hard is it to do this kind of analysis? Is EDS or EELS better?
I have a JEOL 1200 with an older EDS system that I have never used. Could this project be done using this system, or would getting it resurrected be more trouble than finding a lab that does this kind of analysis close to our location? This is a preliminary investigation with no budget.
Anyone want to volunteer to help?
Thanks
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. Visit http://www.aidslifecycle.org for more information about the ride.
==============================Original Headers============================== 11, 17 -- From jmkrupp-at-ucsc.edu Mon Sep 10 16:15:13 2007 11, 17 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8ALFDkm005208 11, 17 -- for {microscopy-at-microscopy.com} ; Mon, 10 Sep 2007 16:15:13 -0500 11, 17 -- Received: from [128.114.125.2] (HELO ucsc.edu) 11, 17 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 11, 17 -- with ESMTPS id 23283902 for microscopy-at-microscopy.com; Mon, 10 Sep 2007 14:15:09 -0700 11, 17 -- Received: from [128.114.25.182] (account jmkrupp-at-ucsc.edu HELO [128.114.25.182]) 11, 17 -- by silver.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 11, 17 -- with ESMTPA id 149581683 for microscopy-at-microscopy.com; Mon, 10 Sep 2007 14:15:08 -0700 11, 17 -- Mime-Version: 1.0 11, 17 -- Message-Id: {p06230902c30b6221d0b2-at-[128.114.25.182]} 11, 17 -- Date: Mon, 10 Sep 2007 14:15:07 -0700 11, 17 -- To: microscopy-at-microscopy.com 11, 17 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 11, 17 -- Subject: plankton analysis 11, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
1. For Quartz specifically this was first published in:
Crossman, GC: Dispersion Staining as Applied to Industrial Hygiene. American Industrial Hygiene Quarterly 18(4):341-344. 1957.
Crossman presented a stereo(macro) use of this technique at a conference in 1963 [IM-1963 in Chicago].
Also, check for cristobalite and tridymite (2 other polymorphs of Silica).
2. For minerals in general it was first published as:
Dodge, Nelson B, "The Darkfield Color Immersion Method" The American Mineralogist 33 (9&10):541-549, 1948.
3. Generally a 10X obj is used for Central stop. For high magnification (which might be necessary in your case), use the darkfield method.
Look up:
"Rediscovery of Darkfield Dispersion Staining while Building a Universal Student Microscope", Microscopy Today, Jan/Feb 2003
If not, email me and I'll pull up and send a pdf of the presentation from Ted and myself. "Critical Darkfield and Its Application to Asbestos Analysis" (with co-presenter Ted Clarke, LaGrange, IN) presented at Inter/Micro-2002, June 24-27, 2002, Chicago, IL.
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
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-----Original Message----- X-from: mckimmye-at-gmail.com [mailto:mckimmye-at-gmail.com] Sent: Monday, September 10, 2007 3:59 PM To: ph2-at-sprynet.com
I got the following helpful answer to my question and wanted to re-post the answer to the group.
Hi Emily, YES there is!!
It's called dispersion staining. You need a special objective with an annular stop and 1.544HD refractive index liquid. The HD stands for high dispersion. You can find instructions in McCrone/Delly's "Polarized Light Microscopy", but basically you establish a cylinder of light slightly smaller than your central stop. Particles with refractive indexes very close to the mounting media will show colored edges depending on the crystal orientation and polarizer orientation. The combination of color, refractive index produces unique characterization. Mind the temperature.
PS: if you have a polarizing filter on your phase contrast scope, you may be able to use a larger stop to get a dark field/dispersion staining effect.
I used to pre-screen clay for alpha quartz at Goodyear Tire and Rubber. If I couldn't find any (2 or 3 preps worth of sample) the sample was clean. If I detected quartz it went to X-ray diffraction I could detect quartz in lower amounts than X-ray diffraction.
I really like dispersion staining, its a great identity and confirmation tool!
Apologies for dual posting - email meltdown lost messages over last few days...
To the person interested in analysing starch structure by confocal, check out Blennow et al. (2003) J Struct Biol 143: 229-241, and more recently Chanzy et al. (2006) J Struct Biol 154: 100-110 for a nice method to do this.
cheers, Rosemary
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
61 2 6246 5475
==============================Original Headers============================== 6, 38 -- From Rosemary.White-at-csiro.au Tue Sep 11 02:12:48 2007 6, 38 -- Received: from vic-MTAout1.csiro.au (vic-MTAout1.csiro.au [150.229.64.37]) 6, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8B7CknE010681 6, 38 -- for {microscopy-at-microscopy.com} ; Tue, 11 Sep 2007 02:12:47 -0500 6, 38 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; 6, 38 -- h=X-IronPort-AV:Received:Received:X-MimeOLE: 6, 38 -- content-class:MIME-Version:Content-Type: 6, 38 -- Content-Transfer-Encoding:Subject:Date:Message-ID: 6, 38 -- X-MS-Has-Attach:X-MS-TNEF-Correlator:Thread-Topic: 6, 38 -- Thread-Index:References:From:To:Return-Path: 6, 38 -- X-OriginalArrivalTime; 6, 38 -- b=OnZ5KIGXx4rmmHsAVskXp729Hh6yWtbOntoT34xDL0WZr0cv2BByV 6, 38 -- fy8u2C35QAARMKL4aw8EtJn6yqbM4cnr/dY+gBybOPfeM16bkKmDt 6, 38 -- A01roTOM1uvRi51PLNzne0; 6, 38 -- X-IronPort-AV: E=Sophos;i="4.20,235,1186322400"; 6, 38 -- d="scan'208";a="147605242" 6, 38 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) 6, 38 -- by vic-ironport-int.csiro.au with ESMTP; 11 Sep 2007 17:12:34 +1000 6, 38 -- Received: from EXACTN1-CBR.nexus.csiro.au ([152.83.131.131]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 6, 38 -- Tue, 11 Sep 2007 17:12:34 +1000 6, 38 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6619.12 6, 38 -- content-class: urn:content-classes:message 6, 38 -- MIME-Version: 1.0 6, 38 -- Content-Type: text/plain; 6, 38 -- charset="Windows-1252" 6, 38 -- Subject: starch analysis in confocal 6, 38 -- Date: Tue, 11 Sep 2007 17:12:34 +1000 6, 38 -- Message-ID: {855FC1242A8B3D48A4F5AA1110B3AF360159A500-at-exactn1-cbr.nexus.csiro.au} 6, 38 -- X-MS-Has-Attach: 6, 38 -- X-MS-TNEF-Correlator: 6, 38 -- Thread-Topic: Confusion about the equation for numerical perture 6, 38 -- Thread-Index: Acf0JXIujlj5QbXmSGGncIzucME9SAAAGCkOAAcrgLk= 6, 38 -- References: {46D81B6B.2080809-at-mpi-cbg.de} {200708311357.CFV42993-at-mpv5.tis.cwru.edu} {46DB28EE.50208-at-auckland.ac.nz} {200709041233.CHB90426-at-mpv6.tis.cwru.edu} {46DDE6FF.8000902-at-auckland.ac.nz} A {395330B7-92E1-4593-A512-97392AF266D2-at-utoronto.ca} {9E1698E170AA254D9FC91CBE697DF1293D9009-at-MAIL2.mcs.usyd.edu.au} A {B8DECDFC-6F16-43D6-BFE7-091925A6DF24-at-utoronto.ca} A {9E1698E170AA254D9FC91CBE697DF1293D900B-at-MAIL2.mcs.usyd.edu.au} 6, 38 -- From: {Rosemary.White-at-csiro.au} 6, 38 -- To: {CONFOCAL-at-LISTSERV.BUFFALO.EDU} , {microscopy-at-microscopy.com} 6, 38 -- X-OriginalArrivalTime: 11 Sep 2007 07:12:34.0540 (UTC) FILETIME=[204F42C0:01C7F443] 6, 38 -- Content-Transfer-Encoding: 8bit 6, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l8B7CknE010681 ==============================End of - Headers==============================
Coming back from the MC2007 conference in Saarbrücken, Germany. I was impressed by a completely new concept of TEM: the low voltage electron microscope (the idea comes from Tchequia).
To summarize very much: - one fix voltage: 5kV - the size of a LM (very short column, very small volume to pump) - no pumps, water cooling and the likes (sort of plug-and-image concept ;-)) - resolution of a few nm - Extreme contrast, even without staining
To understand the concept, think LM: the gun is below, the image of your objet is collected by a detector (YAG) and you observe the detector through binoculars (with further optical magnification) or a camera.
Last but not least: the main disadvantage of the system lies in the limitations of the specimen thickness. With such a low energy, the electrons can't penetrate very deep, so object thicker than 20 nm cannot be imaged (nanotechnologies welcome!). Personally I don't think I could cut sections 20 nm thick, but perhaps some of you super-ultra-microtomists can achieve that.
http://www.dicomps.com/index.php?l=en&p=18&r=171
I have no interest in this company, I just wanted to share my amazement.
Best regards, Stephane .
____________________________________________________________________________________ Need a vacation? Get great deals to amazing places on Yahoo! Travel. http://travel.yahoo.com/
==============================Original Headers============================== 11, 21 -- From nizets2-at-yahoo.com Tue Sep 11 07:27:00 2007 11, 21 -- Received: from web37401.mail.mud.yahoo.com (web37401.mail.mud.yahoo.com [209.191.91.133]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l8BCR0OC032344 11, 21 -- for {microscopy-at-microscopy.com} ; Tue, 11 Sep 2007 07:27:00 -0500 11, 21 -- Received: (qmail 74630 invoked by uid 60001); 11 Sep 2007 12:27:00 -0000 11, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 21 -- s=s1024; d=yahoo.com; 11, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 11, 21 -- b=THxBhJwYwU4EBSUldky7zP2O2MyLVd9PficM2snnpObVQctwZjfVPmm80fQr1xEaloAHKwMjNRD7cZCRqIMhzoJZ1AnjO/u/n/gHJjAzNcE+Azqmi+r7haG+euAgYZiGfBtoZ3Q6G8IFCxABuP2viRsZVRBJ8TS5iDcAwv5frWU=; 11, 21 -- X-YMail-OSG: pjFExqQVM1ndLkjbeQ8B5OpqBuFVx3nDzlwP9yvjn.exMK2V_qrZsV.8IK9vG3wm2hPmK3tzxLKKfcy80Hpg_f2gIeFDEABVF7sCJvYwhyboB_nt7gF7iU0g3jCQhg-- 11, 21 -- Received: from [209.191.118.121] by web37401.mail.mud.yahoo.com via HTTP; Tue, 11 Sep 2007 05:27:00 PDT 11, 21 -- X-Mailer: YahooMailRC/651.50 YahooMailWebService/0.7.134 11, 21 -- Date: Tue, 11 Sep 2007 05:27:00 -0700 (PDT) 11, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 11, 21 -- Subject: low voltage electron microscope 11, 21 -- To: microscopy-at-microscopy.com 11, 21 -- MIME-Version: 1.0 11, 21 -- Content-Type: text/plain; charset=iso-8859-1 11, 21 -- Message-ID: {379611.74487.qm-at-web37401.mail.mud.yahoo.com} 11, 21 -- Content-Transfer-Encoding: 8bit 11, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l8BCR0OC032344 ==============================End of - Headers==============================
Fellow Microscopists, I am searching for a detailed protocol (and reference) to do an electron microscopy in-situ hybridization on a specific RNA species, using digoxigenin. I have a review article and have ordered a textbook, but was hoping to tap into the experts. I would like to do the LR gold post embed hybridization technique. All suggestions deeply appreciated.
} Michael Delannoy } Associate Director, JHMI Microscope Facility } Dept. of Cell Biology } Physiology G-04 } 725 N. Wolfe St. } Baltimore, MD 21205 } (410) 955-1365 office } (410) 614-6890 lab
==============================Original Headers============================== 3, 27 -- From delannoy-at-jhmi.edu Tue Sep 11 08:33:27 2007 3, 27 -- Received: from ipex3.johnshopkins.edu (ipex3.johnshopkins.edu [128.220.161.140]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8BDXRDn013355 3, 27 -- for {microscopy-at-msa.microscopy.com} ; Tue, 11 Sep 2007 08:33:27 -0500 3, 27 -- X-IronPort-AV: E=Sophos;i="4.20,237,1186372800"; 3, 27 -- d="scan'208";a="28897420" 3, 27 -- Received: from jhem1.johnshopkins.edu ([10.181.31.201]) 3, 27 -- by ipex3.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 11 Sep 2007 09:33:27 -0400 3, 27 -- Received: from johnshopkins.edu ([10.181.31.209]) by jesmail.johnshopkins.edu 3, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 3, 27 -- with ESMTP id {0JO700LXDHNSMOM0-at-jesmail.johnshopkins.edu} for 3, 27 -- microscopy-at-msa.microscopy.com; Tue, 11 Sep 2007 09:33:28 -0400 (EDT) 3, 27 -- Received: from [10.181.192.192] (Forwarded-For: [162.129.37.164]) 3, 27 -- by jesmail.johnshopkins.edu (mshttpd); Tue, 11 Sep 2007 09:33:28 -0400 3, 27 -- Date: Tue, 11 Sep 2007 09:33:28 -0400 3, 27 -- From: MICHAEL J DELANNOY {delannoy-at-jhmi.edu} 3, 27 -- Subject: EMISH 3, 27 -- To: "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} 3, 27 -- Message-id: {f409f8e0139c6.46e660e8-at-johnshopkins.edu} 3, 27 -- MIME-version: 1.0 3, 27 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-5.03 (built May 24 2006) 3, 27 -- Content-type: text/plain; charset=us-ascii 3, 27 -- Content-language: en 3, 27 -- Content-transfer-encoding: 7BIT 3, 27 -- Content-disposition: inline 3, 27 -- X-Accept-Language: en 3, 27 -- Priority: normal ==============================End of - Headers==============================
Fellow Microscopists, I am searching for a detailed protocol (and reference) to do an electron microscopy in-situ hybridization on a specific RNA species, using digoxigenin. I have a review article and have ordered a textbook, but was hoping to tap into the experts. I would like to do the LR gold post embed hybridization technique. All suggestions deeply appreciated.
} Michael Delannoy } Associate Director, JHMI Microscope Facility } Dept. of Cell Biology } Physiology G-04 } 725 N. Wolfe St. } Baltimore, MD 21205 } (410) 955-1365 office } (410) 614-6890 lab
==============================Original Headers============================== 3, 27 -- From delannoy-at-jhmi.edu Tue Sep 11 08:33:32 2007 3, 27 -- Received: from ipex4.johnshopkins.edu (ipex4.johnshopkins.edu [128.220.161.141]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8BDXVOS013387 3, 27 -- for {microscopy-at-microscopy.com} ; Tue, 11 Sep 2007 08:33:32 -0500 3, 27 -- X-IronPort-AV: E=Sophos;i="4.20,237,1186372800"; 3, 27 -- d="scan'208";a="21153284" 3, 27 -- Received: from jhem1.johnshopkins.edu ([10.181.31.201]) 3, 27 -- by ipex4.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 11 Sep 2007 09:33:02 -0400 3, 27 -- Received: from johnshopkins.edu ([10.181.31.209]) by jesmail.johnshopkins.edu 3, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 3, 27 -- with ESMTP id {0JO700LNAHN2MOM0-at-jesmail.johnshopkins.edu} for 3, 27 -- microscopy-at-microscopy.com; Tue, 11 Sep 2007 09:33:02 -0400 (EDT) 3, 27 -- Received: from [10.181.192.192] (Forwarded-For: [162.129.37.164]) 3, 27 -- by jesmail.johnshopkins.edu (mshttpd); Tue, 11 Sep 2007 09:33:02 -0400 3, 27 -- Date: Tue, 11 Sep 2007 09:33:02 -0400 3, 27 -- From: MICHAEL J DELANNOY {delannoy-at-jhmi.edu} 3, 27 -- Subject: EMISH 3, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 27 -- Message-id: {f486ccf11425a.46e660ce-at-johnshopkins.edu} 3, 27 -- MIME-version: 1.0 3, 27 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-5.03 (built May 24 2006) 3, 27 -- Content-type: text/plain; charset=us-ascii 3, 27 -- Content-language: en 3, 27 -- Content-transfer-encoding: 7BIT 3, 27 -- Content-disposition: inline 3, 27 -- X-Accept-Language: en 3, 27 -- Priority: normal ==============================End of - Headers==============================
Dear fellow Microscopists: Before we spend days or weeks analyzing our specimens, I hope I could get some feedback on our method of image analysis (Will it hold up to the reviewers?).
Experimental Model: We are implanting a biomaterial through skin in an organ culture system. We want to quantify the epidermal in-growth that occurs into the pores of the biomaterial.
Our method: (all scripted in Photoshop using Fovea Pro) 1. Cryosection (all our material is frozen for antibody purposes) crossectionally from where we first encounter epidermis at the top, to where we see no more epidermis at the bottom. 2. Stain sections for the epidermis with a pankeratin antibody. 3. Capture micrographs. 4. Demarcate the regions of biomaterial and epidermal in-growth. 5. Record area occupied by biomaterial and area occupied by epidermis. 6. Convert data to represent the volume fraction of epidermal ingrowth. 7. Calculate the average distance of in-growth from the perimeter of biomaterial using the Euclidean distance map (255 - mean pixel value X 4 X microns per pixel) 8. Normalize the data to a standard volume. 9. Calculate Òin-growthÓ (normalized volume fraction X average distance of in-growth)
Thus far it has been suggested to: 1. Plug the images into a 3D program to visualize and measure. This presents problems with accurate alignment and seemingly a heck of a lot of work to get back to the data we already have in front of us. 2. Stereology which can extrapolate 3D data out of 2D data, however it seems again we already have the more accurate data in front of us. 3. Micro CT or ultrasound (both havenÕt worked due to low contrast). 4. Digital volumetric imaging, which is time consuming, difficult to measure and so far not working with antibody markers. Making it difficult to demarcate the regions of interest.
So, in this game of quantification, gambling with a postdocÕs time, are we playing with a good hand or are we bluffing?
Thank you for any suggestions.
Robert Underwood University of Washington Dermatology
==============================Original Headers============================== 14, 23 -- From underwoo-at-u.washington.edu Tue Sep 11 11:42:22 2007 14, 23 -- Received: from mxout3.cac.washington.edu (mxout3.cac.washington.edu [140.142.32.166]) 14, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8BGgK7V009234 14, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 11 Sep 2007 11:42:21 -0500 14, 23 -- Received: from hymn10.u.washington.edu (hymn10.u.washington.edu [140.142.13.244]) 14, 23 -- by mxout3.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.06) with ESMTP id l8BGgJwA006672 14, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 14, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 11 Sep 2007 09:42:19 -0700 14, 23 -- Received: from localhost (localhost [127.0.0.1]) 14, 23 -- by hymn10.u.washington.edu (8.13.7+UW06.06/8.13.7+UW07.03) with ESMTP id l8BGgI9n007732 14, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 11 Sep 2007 09:42:18 -0700 14, 23 -- X-Auth-Received: from [128.208.106.224] by hymn10.u.washington.edu via HTTP; Tue, 11 Sep 2007 09:42:18 PDT 14, 23 -- Date: Tue, 11 Sep 2007 09:42:18 -0700 (PDT) 14, 23 -- From: Robert A Underwood {underwoo-at-u.washington.edu} 14, 23 -- To: Microscopy List {Microscopy-at-MSA.Microscopy.Com} 14, 23 -- Subject: [Microscopy] Image analysis question 14, 23 -- Message-ID: {Pine.LNX.4.43.0709110942180.4974-at-hymn10.u.washington.edu} 14, 23 -- MIME-Version: 1.0 14, 23 -- Content-Type: TEXT/PLAIN; charset=CP1252; format=flowed 14, 23 -- X-PMX-Version: 5.3.3.310218, Antispam-Engine: 2.5.2.313940, Antispam-Data: 2007.9.11.92322 14, 23 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__CP_MEDIA_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' 14, 23 -- Content-Transfer-Encoding: 8bit 14, 23 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id l8BGgK7V009234 ==============================End of - Headers==============================
You may recall that the digital camera on our TEM was repossessed by its rightful owner when she moved to a new campus. In the interim, until we can afford a new digital camera, I am back to film.
What a pain. I had forgotten all the hassles. Mix the chemicals, develop the film, load and pump the film, keep track of film numbers and users, shuffle the film into envelopes and bundles to hand back to users. How did I ever do it?
Anyway, until the camera gods smile on us again, I do film. Users still want digital files so I need to find a cheap way to scan their film, hopefully just until we get a new camera.
I have an old Agfa Arcus scanner that was hot stuff 12 years ago when we got it, but it is slooow, and the computer that runs it is even older and lacks features such as a CD writer or USB ports, features expected by modern users.
So, I am looking for a cheap scanner that might be faster and easier for now. Lots of scanners available for $100 - $250, but the ones I have found only do strips of 35 mm film or slides in transparency mode. Anyone know if there is one out there that will accommodate 3.25 x 4" TEM film?
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. Visit http://www.aidslifecycle.org for more information about the ride.
==============================Original Headers============================== 9, 17 -- From jmkrupp-at-ucsc.edu Tue Sep 11 15:11:58 2007 9, 17 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8BKBvAR028123 9, 17 -- for {microscopy-at-microscopy.com} ; Tue, 11 Sep 2007 15:11:57 -0500 9, 17 -- Received: from [128.114.125.5] (HELO ucsc.edu) 9, 17 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 9, 17 -- with ESMTPS id 23327000 for microscopy-at-microscopy.com; Tue, 11 Sep 2007 13:11:57 -0700 9, 17 -- Received: from [128.114.25.182] (account jmkrupp-at-ucsc.edu HELO [128.114.25.182]) 9, 17 -- by copper.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 9, 17 -- with ESMTPA id 149627903 for microscopy-at-microscopy.com; Tue, 11 Sep 2007 13:11:57 -0700 9, 17 -- Mime-Version: 1.0 9, 17 -- Message-Id: {p06230904c30ca44b7eed-at-[128.114.25.182]} 9, 17 -- Date: Tue, 11 Sep 2007 13:11:55 -0700 9, 17 -- To: microscopy-at-microscopy.com 9, 17 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 9, 17 -- Subject: Cheap film scanner? 9, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
We've had good luck with our Epson 4990. Good dpi and more importantly good optical density (OD) range. We set the 3.25 x 4" negs across the 4x5 film holder. There is some bowing of the film, but it works well enough. You could add an additional rib to hold the film up if desired.
(insert usual disclaimers here)
Cheers, Henk
At 04:13 PM 09/11/07, jmkrupp-at-ucsc.edu wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 10, 26 -- From colijn.1-at-osu.edu Tue Sep 11 15:24:31 2007 10, 26 -- Received: from ER6S1.ENG.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8BKOUoL007720 10, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Sep 2007 15:24:31 -0500 10, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 10, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.2-1x11 #31056) 10, 26 -- id {01ML8M64J0S0AAEQ0L-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com; 10, 26 -- Tue, 11 Sep 2007 16:24:28 -0400 (EDT) 10, 26 -- Received: from HOC1.ecr6.ohio-state.edu 10, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu 10, 26 -- (PMDF V6.2-1x11 #31056) 10, 26 -- with ESMTPA id {01ML8M646HMUA9M38Q-at-ecr6.ohio-state.edu} ; Tue, 10, 26 -- 11 Sep 2007 16:24:28 -0400 (EDT) 10, 26 -- Date: Tue, 11 Sep 2007 16:25:59 -0400 10, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 10, 26 -- Subject: Re: [Microscopy] Cheap film scanner? 10, 26 -- In-reply-to: {200709112013.l8BKDwri030661-at-ns.microscopy.com} 10, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 10, 26 -- To: jmkrupp-at-ucsc.edu 10, 26 -- Cc: Microscopy-at-microscopy.com 10, 26 -- Message-id: {7.0.1.0.2.20070911162154.037299a8-at-osu.edu} 10, 26 -- MIME-version: 1.0 10, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 10, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 10, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 10, 26 -- References: {200709112013.l8BKDwri030661-at-ns.microscopy.com} ==============================End of - Headers==============================
John Mackenzie at NC State University NC always has the latest info on scanners. He has always been most helpful to us!
(Probably the latest Epson model!!)
Peter
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-- Peter Ingram Sr. Physicist Adj. Professor of Pathology, Duke University Medical Center Box 90319 LaSalle Street Extension DURHAM NC USA 27708-0319
I put my films on a light box, mask off the "excess" regions with heavy black paper, and photo with an older Canon G3 digital camera. Import into PhotoShop, invert the image (to a positive). Voila!
Geoff
jmkrupp-at-ucsc.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi: } } You may recall that the digital camera on our TEM was repossessed by } its rightful owner when she moved to a new campus. In the interim, } until we can afford a new digital camera, I am back to film. } } What a pain. I had forgotten all the hassles. Mix the chemicals, } develop the film, load and pump the film, keep track of film numbers } and users, shuffle the film into envelopes and bundles to hand back } to users. How did I ever do it? } } Anyway, until the camera gods smile on us again, I do film. Users } still want digital files so I need to find a cheap way to scan their } film, hopefully just until we get a new camera. } } I have an old Agfa Arcus scanner that was hot stuff 12 years ago when } we got it, but it is slooow, and the computer that runs it is even } older and lacks features such as a CD writer or USB ports, features } expected by modern users. } } So, I am looking for a cheap scanner that might be faster and easier } for now. Lots of scanners available for $100 - $250, but the ones I } have found only do strips of 35 mm film or slides in transparency } mode. Anyone know if there is one out there that will accommodate } 3.25 x 4" TEM film? } } Jon }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 8, 28 -- From mcauliff-at-umdnj.edu Tue Sep 11 16:14:57 2007 8, 28 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8BLEvdu032279 8, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Sep 2007 16:14:57 -0500 8, 28 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 8, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id D8355A7B4F 8, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Sep 2007 17:14:56 -0400 (EDT) 8, 28 -- Received: from umdnj.edu (imail.UMDNJ.EDU [130.219.34.129]) 8, 28 -- by zix01.umdnj.edu (Proprietary) with ESMTP id E86F2A7B96 8, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Sep 2007 17:14:54 -0400 (EDT) 8, 28 -- Received: from ([10.32.15.167]) 8, 28 -- by imail.umdnj.edu with ESMTP id KP-BRACD.196603612; 8, 28 -- Tue, 11 Sep 2007 17:14:46 -0400 8, 28 -- MIME-version: 1.0 8, 28 -- Content-transfer-encoding: 7BIT 8, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 8, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 8, 28 -- by umduwc01.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-3.01 (built 8, 28 -- Jul 12 2007; 32bit)) with ESMTP id {0JO800CQT30UPM50-at-umduwc01.umdnj.edu} for 8, 28 -- microscopy-at-microscopy.com; Tue, 11 Sep 2007 17:14:55 -0400 (EDT) 8, 28 -- Message-id: {46E7054A.3020300-at-umdnj.edu} 8, 28 -- Date: Tue, 11 Sep 2007 17:14:50 -0400 8, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 8, 28 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 8, 28 -- To: jmkrupp-at-ucsc.edu, microscopy-at-microscopy.com 8, 28 -- Subject: Re: [Microscopy] Cheap film scanner? 8, 28 -- References: {200709112013.l8BKDKd1029830-at-ns.microscopy.com} 8, 28 -- In-reply-to: {200709112013.l8BKDKd1029830-at-ns.microscopy.com} ==============================End of - Headers==============================
Hi, We started using Roti®-Histokitt II from Carl Roth – Germany as mounting medium. Choice made was evident because we needed : • high refractive index (1.49) • high viscosity (250-450 mPas) • rapid curing (20 min at room temp) • compatibility for fluorescence microscopy at 365nm UV. But after a few 10 days, large air spaces appear systematically between slide and coverslip, destroying the preparation that is intended to be permanent ! We tried by curing at 50°C for one hour but this does not seems to be of any help : problem still arise after a somewhat longer 15 days. Does anyone had similar (bad) experience with this resin ? How did you solved the problem ? Any help is welcome! Or proposal for an other embedding agent with similar physicochemical properties… we are not married with Mr Roth ! Thanks in advance
_________________________
Dr Pascal Veys Project leader - Scientific Attache
Quality of Agricultural Products Department Walloon Agricultural Research Centre - CRA-W Chaussée de Namur, 24 5030 Gembloux (Belgium)
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==============================Original Headers============================== 15, 27 -- From p.veys-at-cra.wallonie.be Wed Sep 12 11:02:40 2007 15, 27 -- Received: from cra.wallonie.be (mail.trace.eu.org [193.190.115.86]) 15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8CG2dcY006188 15, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Sep 2007 11:02:40 -0500 15, 27 -- Received: from PC107 by cra.wallonie.be 15, 27 -- (MDaemon PRO v9.5.3) 15, 27 -- with ESMTP id md50001441552.msg 15, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Sep 2007 18:02:35 +0200 15, 27 -- From: "Pascal Veys" {p.veys-at-cra.wallonie.be} 15, 27 -- To: {Microscopy-at-microscopy.com} 15, 27 -- Subject: problem with Histokitt resin 15, 27 -- Date: Wed, 12 Sep 2007 18:02:41 +0200 15, 27 -- Message-ID: {003801c7f556$5968c720$7d01a8c0-at-PC107} 15, 27 -- MIME-Version: 1.0 15, 27 -- Content-Type: text/plain; 15, 27 -- charset="iso-8859-1" 15, 27 -- X-Mailer: Microsoft Office Outlook 11 15, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 15, 27 -- Thread-Index: Acf1Vlkhki2my+3/SgODs8ET+hcL3g== 15, 27 -- X-Spam-Processed: mail.cra.wallonie.be, Wed, 12 Sep 2007 18:02:35 +0200 15, 27 -- (not processed: message from valid local sender) 15, 27 -- X-Return-Path: p.veys-at-cra.wallonie.be 15, 27 -- X-Envelope-From: p.veys-at-cra.wallonie.be 15, 27 -- X-MDaemon-Deliver-To: Microscopy-at-microscopy.com 15, 27 -- X-MDAV-Processed: mail.cra.wallonie.be, Wed, 12 Sep 2007 18:02:35 +0200 15, 27 -- Content-Transfer-Encoding: 8bit 15, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l8CG2dcY006188 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (isi.forever-at-hotmail.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, September 11, 2007 at 08:20:52 ---------------------------------------------------------------------------
Email: isi.forever-at-hotmail.com Name: Megan Bye
Organization: North Toole County High School
Education: 9-12th Grade High School
Location: Sunburst, Montana, USA
Question: Hello. My Name is Megan Bye and I am a Junior at North Toole County High School in Sunburst, Montana. I participate in an Advanced Research Course which is a challenging program in which students choose science based projects to work on throughout the school year. These projects are then taken to Regional Science Fairs, State Science Fairs,The Intermountain Science Symposium in Salt Lake City, and the International Science and Engineering Fair (ISEF). Last year my teacher,attended the ISEF in New Mexico and saw a demonstration of florescence Imaging to study photosynthesis, but it involved a very expensive apparatus. After hearing about this I have decided that I want to pursue a project using Chlorophyll Fluorescent Imaging. i.e. "The Effect of a selected herbicide on Photosynthesis using Chlorophyll Florescence Imaging" I was wondering what your thoughts were on this idea? Do you think it is possible to do this project with a cheaper device? Do you have any ideas for perhaps using a different technique? Do you know if we could rent or borrow the equipment for a period of time? Do you have any advice for my project? i.e. A different variable that would be more interesting or pertinent? Any thoughts would be greatly appreciated, thank you for your time.
We are interested in examination of a bacterial cell surface. We have two strains of a bug (one is a mutant of the other). One binds environmental proteins to it's surface and one that doesn't. We're interested in looking to see if there are any cell surface differences (It has been suggested that there may be structural cell surface differences in a similar strain of another species). We can't get close enough by SEM to see anything useful... Is this the kind of thing that could be examined by AFM perhaps? Does anyone here have experience with the examination of bacterial cell surface structures by AFM?
Cheers,
-- Scott J. Coutts ------------------------------------------------------------------------- Bacterial Pathogenesis Research Group Department of Microbiology Box 53, Monash University, 3800, Australia Phone +61 3 9905 8592, Fax +61 3 9905 4811 -------------------------------------------------------------------------
==============================Original Headers============================== 4, 26 -- From scott.coutts-at-med.monash.edu.au Wed Sep 12 20:14:59 2007 4, 26 -- Received: from kyle.its.monash.edu.au (kyle.its.monash.edu.au [130.194.13.163]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8D1EwxR009772 4, 26 -- for {microscopy-at-microscopy.com} ; Wed, 12 Sep 2007 20:14:58 -0500 4, 26 -- Received: from larry.its.monash.edu.au ([130.194.13.82]) 4, 26 -- by kyle.its.monash.edu.au 4, 26 -- (Sun Java System Messaging Server 6.2-4.03 (built Sep 22 2005)) 4, 26 -- with ESMTP id {0JOA009E88SXOVH0-at-kyle.its.monash.edu.au} for 4, 26 -- microscopy-at-microscopy.com; Thu, 13 Sep 2007 11:14:57 +1000 (EST) 4, 26 -- Received: from larry.its.monash.edu.au (localhost.localdomain [127.0.0.1]) 4, 26 -- by localhost (Postfix) with SMTP id 1827F80002 for 4, 26 -- {microscopy-at-microscopy.com} ; Thu, 13 Sep 2007 11:14:57 +1000 (EST) 4, 26 -- Received: from [130.194.200.122] 4, 26 -- (MicrobSCouttsDT.med.monash.edu.au [130.194.200.122]) 4, 26 -- by larry.its.monash.edu.au (Postfix) with ESMTP id 05A083C00C for 4, 26 -- {microscopy-at-microscopy.com} ; Thu, 13 Sep 2007 11:14:57 +1000 (EST) 4, 26 -- Date: Thu, 13 Sep 2007 11:15:24 +1000 4, 26 -- From: Scott Coutts {scott.coutts-at-med.monash.edu.au} 4, 26 -- Subject: AFM of bacteral cell surfaces? 4, 26 -- To: microscopy-at-microscopy.com 4, 26 -- Message-id: {46E88F2C.5050409-at-med.monash.edu.au} 4, 26 -- Organization: Monash University 4, 26 -- MIME-version: 1.0 4, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 4, 26 -- Content-transfer-encoding: 7bit 4, 26 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) ==============================End of - Headers==============================
Dear Scott, I think that it is reasonable to try AFM to investigate bacterial cell surfaces, but I have not done this myself. regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] ============================================= ----- Original Message ----- From: scott.coutts-at-med.monash.edu.au To: donc-at-asmicro.com Sent: Wednesday, September 12, 2007 9:18 PM Subject: [a] [Microscopy] AFM of bacteral cell surfaces?
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Hi All,
We are interested in examination of a bacterial cell surface. We have two strains of a bug (one is a mutant of the other). One binds environmental proteins to it's surface and one that doesn't. We're interested in looking to see if there are any cell surface differences (It has been suggested that there may be structural cell surface differences in a similar strain of another species). We can't get close enough by SEM to see anything useful... Is this the kind of thing that could be examined by AFM perhaps? Does anyone here have experience with the examination of bacterial cell surface structures by AFM?
Cheers,
-- Scott J. Coutts ------------------------------------------------------------------------- Bacterial Pathogenesis Research Group Department of Microbiology Box 53, Monash University, 3800, Australia Phone +61 3 9905 8592, Fax +61 3 9905 4811 -------------------------------------------------------------------------
==============================Original Headers============================== 4, 26 -- From scott.coutts-at-med.monash.edu.au Wed Sep 12 20:14:59 2007 4, 26 -- Received: from kyle.its.monash.edu.au (kyle.its.monash.edu.au [130.194.13.163]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8D1EwxR009772 4, 26 -- for {microscopy-at-microscopy.com} ; Wed, 12 Sep 2007 20:14:58 -0500 4, 26 -- Received: from larry.its.monash.edu.au ([130.194.13.82]) 4, 26 -- by kyle.its.monash.edu.au 4, 26 -- (Sun Java System Messaging Server 6.2-4.03 (built Sep 22 2005)) 4, 26 -- with ESMTP id {0JOA009E88SXOVH0-at-kyle.its.monash.edu.au} for 4, 26 -- microscopy-at-microscopy.com; Thu, 13 Sep 2007 11:14:57 +1000 (EST) 4, 26 -- Received: from larry.its.monash.edu.au (localhost.localdomain [127.0.0.1]) 4, 26 -- by localhost (Postfix) with SMTP id 1827F80002 for 4, 26 -- {microscopy-at-microscopy.com} ; Thu, 13 Sep 2007 11:14:57 +1000 (EST) 4, 26 -- Received: from [130.194.200.122] 4, 26 -- (MicrobSCouttsDT.med.monash.edu.au [130.194.200.122]) 4, 26 -- by larry.its.monash.edu.au (Postfix) with ESMTP id 05A083C00C for 4, 26 -- {microscopy-at-microscopy.com} ; Thu, 13 Sep 2007 11:14:57 +1000 (EST) 4, 26 -- Date: Thu, 13 Sep 2007 11:15:24 +1000 4, 26 -- From: Scott Coutts {scott.coutts-at-med.monash.edu.au} 4, 26 -- Subject: AFM of bacteral cell surfaces? 4, 26 -- To: microscopy-at-microscopy.com 4, 26 -- Message-id: {46E88F2C.5050409-at-med.monash.edu.au} 4, 26 -- Organization: Monash University 4, 26 -- MIME-version: 1.0 4, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 4, 26 -- Content-transfer-encoding: 7bit 4, 26 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) ==============================End of - Headers==============================
==============================Original Headers============================== 12, 24 -- From donc-at-asmicro.com Wed Sep 12 21:07:23 2007 12, 24 -- Received: from smtp109.sbc.mail.re2.yahoo.com (smtp109.sbc.mail.re2.yahoo.com [68.142.229.96]) 12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l8D27Ng5022358 12, 24 -- for {microscopy-at-microscopy.com} ; Wed, 12 Sep 2007 21:07:23 -0500 12, 24 -- Received: (qmail 50553 invoked from network); 13 Sep 2007 02:07:23 -0000 12, 24 -- Received: from unknown (HELO asm15) (asmicro-at-sbcglobal.net-at-68.58.145.13 with login) 12, 24 -- by smtp109.sbc.mail.re2.yahoo.com with SMTP; 13 Sep 2007 02:07:22 -0000 12, 24 -- X-YMail-OSG: sFYj874VM1mKPJ_tqU1kt6gocRS5JFTsAyCm5ooMobzWKrvyrZiI.YHG_ceh93cXA08Aox36cDGPMrkoi1AwYqV2Ej7xBgKKgTjIidxHAMo2Mp7RHgmxEuIjA.CFgJSiqUOQ3oTtCulU5jOwHD7Xxg8DdnA3nH79BreOXcPkuGjpw1BxX7RawYdg14v8LceuZDM4WIHE 12, 24 -- Message-ID: {001301c7f5aa$cf2e1e20$0202a8c0-at-asm15} 12, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 12, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com} 12, 24 -- References: {200709130118.l8D1IbTF014736-at-ns.microscopy.com} 12, 24 -- Subject: Re: [a] [Microscopy] AFM of bacteral cell surfaces? 12, 24 -- Date: Wed, 12 Sep 2007 22:07:11 -0400 12, 24 -- MIME-Version: 1.0 12, 24 -- Content-Type: text/plain; 12, 24 -- format=flowed; 12, 24 -- charset="iso-8859-1"; 12, 24 -- reply-type=original 12, 24 -- Content-Transfer-Encoding: 7bit 12, 24 -- X-Priority: 3 12, 24 -- X-MSMail-Priority: Normal 12, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 12, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 ==============================End of - Headers==============================
this question is difficult to answer because we don't know the type of bug, and, in particular, the type of preparation that was used for SEM. } We can't get close enough by SEM to see anything useful. This can mean that the SEM is limiting, but - more likely - that the method to prepare the samples for SEM was the limiting factor. Most protocols still in use today treat the cell surface of prokaryotic cells in a way that the structures under investigation are damaged or completely removed. This would - in a similar way - also limit the visibility of structures by TEM or in the AFM (or any similar instrument).
alternative routes: 1) cryo-preparation, followed by RT-SEM 2) cryo-preparation, followed by cryo-SEM 3) 'suitable' preparation method, followed by AFM (or similar instrument) 4) quick freezing, freeze-etching, followed by TEM - this can nicely be used for visualizing surface structure of prokaryotic cells 5) cryo-preparation (eg HPF+FS + resin embedding), followed by sectioning and visualizing in TEM
all not easy to do - but one of these may give an answer.
kind regards, Reinhard Rachel ---------------------- PD Dr. Reinhard Rachel Universitaet Regensburg Centre for EM - NWF III - -at-Institute for Anatomy Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720, 1666(TEM) fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-r.de
==============================Original Headers============================== 7, 23 -- From reinhard.rachel-at-biologie.uni-regensburg.de Thu Sep 13 03:17:37 2007 7, 23 -- Received: from rrzmta1.rz.uni-regensburg.de (rrzmta1.rz.uni-regensburg.de [194.94.155.51]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8D8HbGT008963 7, 23 -- for {Microscopy-at-Microscopy.Com} ; Thu, 13 Sep 2007 03:17:37 -0500 7, 23 -- Received: from rrzmta1.rz.uni-regensburg.de (localhost [127.0.0.1]) 7, 23 -- by localhost (Postfix) with SMTP id 7656DA7B0 7, 23 -- for {Microscopy-at-Microscopy.Com} ; Thu, 13 Sep 2007 10:17:39 +0200 (CEST) 7, 23 -- Received: from localhost (donald1.rz.uni-regensburg.de [132.199.4.91]) 7, 23 -- by rrzmta1.rz.uni-regensburg.de (Postfix) with ESMTP id 662D4A789 7, 23 -- for {Microscopy-at-Microscopy.Com} ; Thu, 13 Sep 2007 10:17:39 +0200 (CEST) 7, 23 -- Received: from pc90678.biologie.uni-regensburg.de (pc90678.biologie.uni-regensburg.de [132.199.82.154]) 7, 23 -- by webmail.uni-regensburg.de (IMP) with HTTP 7, 23 -- for {rar04520-at-rrzlic2.uni-regensburg.de} ; Thu, 13 Sep 2007 10:17:36 +0200 7, 23 -- Message-ID: {1189671456.46e8f21ff1902-at-webmail.uni-regensburg.de} 7, 23 -- Date: Thu, 13 Sep 2007 10:17:36 +0200 7, 23 -- From: reinhard rachel {reinhard.rachel-at-biologie.uni-regensburg.de} 7, 23 -- To: Microscopy-at-Microscopy.Com 7, 23 -- Subject: [Microscopy] AFM of bacteral cell surfaces 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; charset=ISO-8859-1 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 7, 23 -- X-Originating-IP: 132.199.82.154 ==============================End of - Headers==============================
The scope is fine at 50 KV but not at 80KV - during shake out at 80KV the beam is visible on the screen but then goes completely off the screen towards the 1:00 position. When I switch from image to spectrum there is no spectrometer arrow no matter how much I push the shift key. I changed out the high voltage tank but that did not cure the problem. Is it the filament? Lenses? A bad potentiometer? All of the above? ;-)
Any suggestions/thoughts/ideas would be greatly appreciated. best, Beth
********************************************************************** Beth Richardson Electron Microscopy Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
http://www.plantbio.uga.edu/emlab/
Phone - (706) 542-1790 & FAX - (706) 542-1805
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
************************************************************************ *** The Friends of the Marine Institute - Join Today! www.friendsofugami.org
==============================Original Headers============================== 12, 19 -- From beth-at-plantbio.uga.edu Thu Sep 13 09:45:05 2007 12, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 12, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8DEj5OY006582 12, 19 -- for {microscopy-at-microscopy.com} ; Thu, 13 Sep 2007 09:45:05 -0500 12, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 12, 19 -- (authenticated user beth-at-plantbio.uga.edu) 12, 19 -- by dogwood.plantbio.uga.edu 12, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 12, 19 -- for microscopy-at-microscopy.com; 12, 19 -- Thu, 13 Sep 2007 10:45:01 -0400 12, 19 -- Mime-Version: 1.0 (Apple Message framework v752.3) 12, 19 -- Content-Transfer-Encoding: 7bit 12, 19 -- Message-Id: {39BAD8A9-07FC-47A3-AAD9-9E792FBB7BC7-at-plantbio.uga.edu} 12, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 12, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 12, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 12, 19 -- Subject: Zeiss 902 blues 12, 19 -- Date: Thu, 13 Sep 2007 10:45:05 -0400 12, 19 -- X-Mailer: Apple Mail (2.752.3) ==============================End of - Headers==============================
Does anyone know of a source of antibodies to beta-galactosidase and green fluorescent protein that they know work for immuno-EM on LR White, LR Gold, or PLT/HM20 low [aldehde] and low temperature processed material ? Cheers B ********************************************************************** Brent Gowen Electron Microscopy Laboratory Department of Biology University of Victoria P.O. Box 3020 STN CSC Victoria, BC, Canada V8W 3N5 Tel: (250)-721-7132 http://web.uvic.ca/em-lab/
==============================Original Headers============================== 4, 25 -- From bgowen-at-uvic.ca Thu Sep 13 11:08:04 2007 4, 25 -- Received: from castle.comp.uvic.ca (castle.comp.uvic.ca [142.104.5.97]) 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8DG84Sx020434 4, 25 -- for {microscopy-at-microscopy.com} ; Thu, 13 Sep 2007 11:08:04 -0500 4, 25 -- Received: from wm3.uvic.ca (tamarin.comp.uvic.ca [142.104.148.232]) 4, 25 -- by castle.comp.uvic.ca (8.13.8/8.13.8) with ESMTP id l8DG83kY13439108 4, 25 -- for {microscopy-at-microscopy.com} ; Thu, 13 Sep 2007 09:08:03 -0700 4, 25 -- Received: from 142.104.193.193 (proxying for 142.104.208.11) 4, 25 -- (SquirrelMail authenticated user bgowen) 4, 25 -- by wm3.uvic.ca with HTTP; 4, 25 -- Thu, 13 Sep 2007 08:44:27 -0700 (PDT) 4, 25 -- Message-ID: {1259.142.104.193.193.1189698267.squirrel-at-wm3.uvic.ca} 4, 25 -- Date: Thu, 13 Sep 2007 08:44:27 -0700 (PDT) 4, 25 -- Subject: primaries 4, 25 -- From: "bgowen" {bgowen-at-uvic.ca} 4, 25 -- To: microscopy-at-microscopy.com 4, 25 -- User-Agent: SquirrelMail/1.4.9a 4, 25 -- MIME-Version: 1.0 4, 25 -- Content-Type: text/plain;charset=iso-8859-1 4, 25 -- Content-Transfer-Encoding: 8bit 4, 25 -- X-Priority: 3 (Normal) 4, 25 -- Importance: Normal 4, 25 -- X-UVic-Virus-Scanned: OK - Passed virus scan by Sophos (sophie) on castle 4, 25 -- X-UVic-Spam-Scan: castle.comp.uvic.ca Not_scanned_LOCAL 4, 25 -- X-Scanned-By: MIMEDefang 2.33 (www . roaringpenguin . com / mimedefang) ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (jjb42-at-pitt.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, September 13, 2007 at 11:55:04 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both jjb42-at-pitt.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: jjb42-at-pitt.edu Name: Justin
Organization: Univerisity of Pittsburgh
Education: Graduate College
Location: Pittsburgh, PA, US
Title: tilt correction
Question: I have a periodic 2 dimensional image which is tilted. I'm working with a Phillips XL30 and tried to correct for this tilt through tilting the stage and using the tilt correction feature. Tilting the stage has no effect on the periodicity of the 2D image but using the tilt correction feature does. What exactly is the tilt correction feature doing here?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kirchoff-at-uncg.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kirchoff-at-uncg.edu Name: Bruce
Organization: Univeristy of North Carolina at Greensboro
Title-Subject: [Filtered] Camera comarison
Question: I am about to purchase a 5 megapixel digital camera for my Ortholux II. I have quotes from Olympus (both Q-Imaging and Olympus brands), Nikon and Motic (via. Micro-Optics). I have seen the Nikon camera but not the others. Can you recommend one of these cameras over the others, or is there a fourth supplier I should be considering? In addition to the camera and adapter, I need software that permits extended depth of focus. All of the manufacturers say that they provide this, but the quality may vary. Even different versions of NIS-Elements (Nikon) vary in their ability to stack images.
The total cost of the system must be under $7,000. This excludes some of the Olympus cameras.
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Email: carnahan-at-edison-labs.com Name: James Carnahan
Organization: Edison Analytical Laboratories, Inc.
Title-Subject: [Filtered] Film camera for Jeol 100-S TEM
Question: We have recently acquired a Jeol 100-S TEM that has only the standard bottom mount sheet film camera cassettes. We are looking for a 35 mm ( side mount) camera and hoped that someone has upgraded a Jeol 100 series to digital and has an old camera/scintillator sitting unused on the shelf that might be available. The flange blanking plate is 4 1/2 x 2 1/4".
Regards,
Jim Carnahan Edison Analytical Labs (518) 393-2112 carnahan-at-edison-labs.com
Have any of you with Leo 1400 series SEM had any issues with instrument software?
I am having trouble with configuration files changing for no reason. Such as losing what detectors are on the machine or size of the monitor being used. Most recent issue is the loss of the signal mixing function under the detector tab with the second detector drop down list being grayed out.
System is running under Windows 2000 and is scanned often for spy ware and virus.
Any help would be appreciated.
Roy Beavers Southern Methodist University rbeavers-at-smu.edu
==============================Original Headers============================== 7, 23 -- From rbeavers-at-mail.smu.edu Thu Sep 13 15:49:29 2007 7, 23 -- Received: from s31xub.systems.smu.edu (s31xub.systems.smu.edu [129.119.70.73]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8DKnTNv010573 7, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Sep 2007 15:49:29 -0500 7, 23 -- Received: from s31xe7.systems.smu.edu ([129.119.70.140]) by s31xub.systems.smu.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 23 -- Thu, 13 Sep 2007 15:49:24 -0500 7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="US-ASCII" 7, 23 -- Subject: Leo 1450VPSE Software Problem 7, 23 -- Date: Thu, 13 Sep 2007 15:49:24 -0500 7, 23 -- Message-ID: {F8AF6BF6CD1CC040AF35B0C2D1680BBB021A70D4-at-s31xe7.systems.smu.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- Thread-Topic: Leo 1450VPSE Software Problem 7, 23 -- Thread-Index: Acf2R5Fi78Z3fg9nQ2qxoHT9a15GvA== 7, 23 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 7, 23 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 13 Sep 2007 20:49:24.0943 (UTC) FILETIME=[919CC5F0:01C7F647] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l8DKnTNv010573 ==============================End of - Headers==============================
How old is your C: drive? When was the last time you ran scandisk?
If you have an old drive, it could be going bad. Check Computer Management and the Event log to see if it reports drive errors. If it does, then you need to migrate to a new drive.
gary g.
At 12:50 PM 9/13/2007, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 6, 20 -- From gary-at-gaugler.com Thu Sep 13 17:19:34 2007 6, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l8DMJXZ6024333 6, 20 -- for {microscopy-at-microscopy.com} ; Thu, 13 Sep 2007 17:19:33 -0500 6, 20 -- Message-Id: {200709132219.l8DMJXZ6024333-at-ns.microscopy.com} 6, 20 -- Received: (qmail 7795 invoked from network); 13 Sep 2007 15:19:32 -0700 6, 20 -- Received: by simscan 1.1.0 ppid: 7791, pid: 7792, t: 0.1326s 6, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 6, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 6, 20 -- by qsmtp4 with SMTP; 13 Sep 2007 15:19:32 -0700 6, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 6, 20 -- Date: Thu, 13 Sep 2007 15:19:46 -0800 6, 20 -- To: rbeavers-at-mail.smu.edu 6, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 6, 20 -- Subject: Re: [Microscopy] Leo 1450VPSE Software Problem 6, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 6, 20 -- In-Reply-To: {200709132050.l8DKos3P013312-at-ns.microscopy.com} 6, 20 -- References: {200709132050.l8DKos3P013312-at-ns.microscopy.com} 6, 20 -- Mime-Version: 1.0 6, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-427C24F ==============================End of - Headers==============================
Dear Justin, The two methods do very different things. The tilt on the stage will physically tilt the stage so that you can make your sample exactly normal to the beam. I find that small tilts of the stage make very little difference to the image, because of the large depth of focus of the SEM. The "tilt correction" of the SEM electronics is a manipulation of the X and Y rasters to electronically compensate for a tilted stage. In the old days we often used the SEM with the stage tilted 45 degrees towards the SEM detector, to improve the signal. If measurements on a flat surface were important, the "tilt correction" could take a square grid and remove the foreshortening caused by the stage tilt. You can imagine what that would do to a sphere. My suggestion is to use the stage tilt to make the sample as normal to the beam as possible and avoid electronic tilt correction. Put a 2D calibration sample into the SEM with your sample to check the results. Regards,
-----Original Message----- X-from: jjb42-at-pitt.edu [mailto:jjb42-at-pitt.edu] Sent: September 13, 2007 1:39 PM To: maryflet-at-interchange.ubc.ca
This Question was submitted to Ask-A-Microscopist by (jjb42-at-pitt.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, September 13, 2007 at 11:55:04 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both jjb42-at-pitt.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: jjb42-at-pitt.edu Name: Justin
Organization: Univerisity of Pittsburgh
Education: Graduate College
Location: Pittsburgh, PA, US
Title: tilt correction
Question: I have a periodic 2 dimensional image which is tilted. I'm working with a Phillips XL30 and tried to correct for this tilt through tilting the stage and using the tilt correction feature. Tilting the stage has no effect on the periodicity of the 2D image but using the tilt correction feature does. What exactly is the tilt correction feature doing here?
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Email: gmcredo-at-yahoo.com Name: Grace Credo
Organization: Intel Research
Title-Subject: [Filtered] Cell Robotics LaserTweezers system question
Question: Have a copy of the user manual or (even better) a PDF copy of the user manual?
We have one of these systems in our research lab, but can't locate our old copy of the user manual and would like to use it. This company appears to be out of business and we can't reach them. Can anyone help?
Thanks! Grace Research Scientist Intel Research Santa Clara, CA
} I find that small tilts of the stage make very little difference } to the image, because of the large depth of focus of the SEM.
This will depend on what is being imaged. "Tilt Correction" is ideally suited to planar/near planar specimens.
Condsider an integrated circuit composed of a series of perpendicular tracks. Image it untilted and measurements in X and Y should be the same scale - track widths and seperation constant.
Tilt the IC to a high angle, with tilt axis parallel to one set of tracks, and the apparent width of these tracks will decrease - whilst the others will remain constant regardless of angle of tilt. In effect the magnification differs in the 2 directions.
} What exactly is the tilt correction feature doing here?
The "Tilt Correction" usually operates by cramming in more scanned lines along either X or Y - so that they are a constant distance apart on the specimen surface. Thus compensating for the change in magnification. Applying a tilt correction to an image of a flat specimen, tilted along an axis parallel to a scan direction, will produce a corrected image on which true measurements can be made despite the tilt and regardless of the orientation of the features being measured.
} Tilting the stage has no effect on the periodicity of the 2D image } but using the tilt correction feature does.
However if its a more 3 dimensional object you are looking at things may be very different. To take another extreme case: spherical particles will appear round regardless of the angles at which they are viewed. They will still appear as spheres when tilted. Use the tilt correction on these and the image will be distorted rather than corrected.... There are pictures of just this effect in some SEM text books - there must be images of this type on the web somewhere - anyone know where ?
So the effect and use of stage tilt and tilt correction both depend on what you are looking at - and what information you want.
In the old days } we often used the SEM with the stage tilted 45 degrees towards the SEM } detector, to improve the signal.
So doesn't this help on newer SEM's ?
A useful side effect of the distortion produced by specimen tilt is that the increased magnification in one direction allows surface irregularities to be seen more clearly in some types of specimens.
-- AB78-at-ESC.CAM.AC.UK DEPARTMENT OF EARTH SCIENCES UNIVERSITY OF CAMBRIDGE Tel +44 1223 333469 DOWNING STREET +44 1223 333400 CAMBRIDGE FAX +44 1223 333450 CB2 3EQ
Manager of XRD discussion list: http://www.jiscmail.ac.uk/lists/xrd.html
} } I find that small tilts of the stage make very little } } difference } to the image, because of the large depth of } } focus of the SEM. } } This will depend on what is being imaged. "Tilt Correction" } is ideally suited to planar/near planar specimens.
Careful of what you're speaking of. I believe the original post was regarding "tilt correction", which corrects for the foreshortening along the tilt axis -- i.e., making tilted geometry appear flat. The other aspect of a tilt is accommodating the longer and shorter focal distances for a tilted flat sample, which should be referred to as "dynamic focus".
cheerios, michael shaffer :o) SEM-MLA Research Coodinator INCO Innovation Centre Memorial University St. John's Newfoundland http://www.mun.ca/creait/maf/
==============================Original Headers============================== 5, 21 -- From michael-at-shaffer.net Fri Sep 14 10:03:52 2007 5, 21 -- Received: from n007.sc1.he.tucows.com (smtpout1480.sc1.he.tucows.com [64.97.157.180]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8EF3qgt002498 5, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Sep 2007 10:03:52 -0500 5, 21 -- Received: from roamingwolf (134.153.130.141) by n007.sc1.he.tucows.com (7.2.069.1) (authenticated as michael-at-shaffer.net) 5, 21 -- id 465670BB01444C98; Fri, 14 Sep 2007 15:03:42 +0000 5, 21 -- From: "michael shaffer" {michael-at-shaffer.net} 5, 21 -- To: {ab78-at-esc.cam.ac.uk} 5, 21 -- Cc: "MSA Microscopy list" {Microscopy-at-microscopy.com} 5, 21 -- References: {200709140928.l8E9S0ts016636-at-ns.microscopy.com} 5, 21 -- Subject: RE: [Microscopy] AskAMicroscopist: SEM tilt correction 5, 21 -- Date: Fri, 14 Sep 2007 12:35:28 -0230 5, 21 -- Message-ID: {002301c7f6e0$b0f657b0$8d829986-at-CREAIT.MUN.CA} 5, 21 -- MIME-Version: 1.0 5, 21 -- Content-Type: text/plain; 5, 21 -- charset="us-ascii" 5, 21 -- Content-Transfer-Encoding: 7bit 5, 21 -- X-Mailer: Microsoft Office Outlook 11 5, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 5, 21 -- thread-index: Acf2sMHVCE8RFpdGTsiqWpsPO4LBBAAL00/g 5, 21 -- In-Reply-To: {200709140928.l8E9S0ts016636-at-ns.microscopy.com} ==============================End of - Headers==============================
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Email: mbisher-at-princeton.edu Name: Margaret Bisher
Organization: Princeton University
Title-Subject: [Filtered] Offer: TEM Film Racks
Question: I've been doing some cleaning around the lab and decided that I don't need to keep the dozen acrylic film racks that I used in the darkroom for my TEM film. I am going to keep a few, but I have 7 that I will give away - for FREE-. I hate to throw them away, somebody out there might want them.
Just let me know if you are interested and I will be more than happy to send them to you.
Thanks, Peggy Bisher
Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher-at-princeton.edu
I have a sample of SDS-PAGE gel which I hope contains CdS nanoparticles attached to a phytochelatin. I would like to examine the particles in the TEM, but I am at a loss as to how to prepare them. Does anyone have any experience with extracting proteins from these gels? It has also been suggested that I treat the gel as a biological specimen (fix, dehydrate, embed, section). Does anyone have any tips they would be willing to share?
Thanks in advance,
Katie.
------------------------------- Katie Levick
Technical Officer Electron Microscope Unit UNSW Analytical Centre University of New South Wales Sydney NSW
02 9385 6390 k.levick-at-unsw.edu.au
==============================Original Headers============================== 9, 29 -- From k.levick-at-unsw.edu.au Mon Sep 17 01:21:28 2007 9, 29 -- Received: from smtp-dist.unsw.edu.au (smtp-dist-03.services.comms.unsw.EDU.AU [149.171.97.18]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8H6LQRb013671 9, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 17 Sep 2007 01:21:27 -0500 9, 29 -- Received: from localhost (avspam-01.services.comms.unsw.edu.au [149.171.100.16]) 9, 29 -- by smtp-dist.unsw.edu.au (8.13.6/8.13.6) with ESMTP id l8H6LQDT019989 9, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 17 Sep 2007 16:21:26 +1000 (EST) 9, 29 -- Received: from smtp.unsw.edu.au ([127.0.0.1]) 9, 29 -- by localhost (slag.comms.unsw.edu.au [127.0.0.1]) (amavisd-new, port 10025) 9, 29 -- with ESMTP id xqExRhLQPvi8 for {Microscopy-at-Microscopy.Com} ; 9, 29 -- Mon, 17 Sep 2007 16:21:25 +1000 (EST) 9, 29 -- Received: from AC026 ([129.94.150.26]) 9, 29 -- by smtp.unsw.edu.au (8.13.6/8.13.6) with SMTP id l8H6LPrp011995 9, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 17 Sep 2007 16:21:25 +1000 (EST) 9, 29 -- Message-ID: {008601c7f8f3$00be4780$1a965e81-at-AC026} 9, 29 -- From: "Katie Levick" {k.levick-at-unsw.edu.au} 9, 29 -- To: {Microscopy-at-Microscopy.Com} 9, 29 -- Subject: microscopy of SDS-page gel 9, 29 -- Date: Mon, 17 Sep 2007 16:21:37 +1000 9, 29 -- MIME-Version: 1.0 9, 29 -- Content-Type: text/plain; 9, 29 -- format=flowed; 9, 29 -- charset="iso-8859-1"; 9, 29 -- reply-type=original 9, 29 -- Content-Transfer-Encoding: 7bit 9, 29 -- X-Priority: 3 9, 29 -- X-MSMail-Priority: Normal 9, 29 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 9, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 ==============================End of - Headers==============================
Dear listers, I have a Philips EM400t TEM and a Camscan SEM which I need to dispose of so I can install a new machine (dualbeam FIB, yummy). Neither have been in working condition for a few years although I suspect with a small amount of effort they could be made operational. Both have EDX units (again, no idea of their functionality). Does anyone have any advice they can share with me on this? I have a quote from a specialist company which seems quite expensive; the local scrap yard is interested in the copper and lead but won't touch some parts (the oil in the HT tank, for instance). I've no objections to getting my hands dirty and dismantling them myself, in fact it would be interesting to have a good look at the innards of the beasts...
Many thanks
Richard
Richard Beanland Integrity Scientific Ltd www. integrityscientific.com
==============================Original Headers============================== 5, 25 -- From contact-at-integrityscientific.com Mon Sep 17 04:37:10 2007 5, 25 -- Received: from em-p07-ob.rzone.de (em-p07-ob.rzone.de [81.169.146.245]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8H9bAFr029635 5, 25 -- for {microscopy-at-microscopy.com} ; Mon, 17 Sep 2007 04:37:10 -0500 5, 25 -- Received: from post.webmailer.de (sek.store [192.168.40.124]) 5, 25 -- by bjorn-em-02.store (RZmta 12.10) with ESMTP id J00f51j8GLWXrc 5, 25 -- for {microscopy-at-microscopy.com} ; Mon, 17 Sep 2007 11:37:09 +0200 (MEST) 5, 25 -- (envelope-from: {contact-at-integrityscientific.com} ) 5, 25 -- Received: (from httpd-at-localhost) 5, 25 -- by post.webmailer.de (8.13.6/8.13.6) id l8H9b9nV001807 5, 25 -- for microscopy-at-microscopy.com; Mon, 17 Sep 2007 11:37:09 +0200 (MEST) 5, 25 -- Date: Mon, 17 Sep 2007 11:37:09 +0200 (MEST) 5, 25 -- Message-Id: {200709170937.l8H9b9nV001807-at-post.webmailer.de} 5, 25 -- X-Authentication-Warning: sek.store: httpd set sender to contact-at-integrityscientific.com using -f 5, 25 -- To: microscopy-at-microscopy.com 5, 25 -- From: "Richard Beanland" {contact-at-integrityscientific.com} 5, 25 -- Subject: Instrument disposal in the UK - SEM and TEM 5, 25 -- X-Priority: 3 5, 25 -- X-Abuse: 982623 / 131.111.102.43 5, 25 -- X-RZG-MBID: 15cBjAKTcdbsEQ== 5, 25 -- MIME-Version: 1.0 5, 25 -- Content-Type: text/plain; charset="ISO-8859-1" 5, 25 -- X-RZG-CLASS-ID: em07 5, 25 -- Content-Transfer-Encoding: 8bit 5, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l8H9bAFr029635 ==============================End of - Headers==============================
An immediate permanent staff position is open for a scanning electron microscopist at the Electron Probe Instrumentation Center (EPIC) housed within the NUANCE center of Northwestern University in Evanston, IL. The EPIC facility includes four fully loaded SEMs, three TEMs and a FEI Helios dual beam FIB. In addition to EPIC, NUANCE hosts many other instrumentations and capabilities for advanced characterization.
Primary responsibilities for this position include training new SEM users and supporting their skill development; providing technical support and collaborative assistance to users on their projects; minor maintenance of the instruments and specimen preparation accessories, and analysis for external users.
Qualifications include a BS or equivalent technical training in science/engineering discipline.
Desired skills include: Prior experience in operation of modern SEMs and analytical accessories, strong computer and communication skills, some knowledge of modern electronics, and a background in physical sciences, such as materials science. Ability to instruct others on instrument use, scientific principles, and lab safety. Desire to work with state-of-the-art characterization instruments in a dynamic and vibrant research environment. Demonstrated ability to learn new information quickly and to be able to assimilate it into a base of knowledge and to work with minimal supervision.
Salary will be commensurate with experience and credentials. This position enjoys all the eligible benefits for staff of Northwestern University, which can be reviewed at: {http://www.northwestern.edu/hr/benefits/}
Please send documents, including a resume, list of three references with email addresses and telephone numbers, and salary requirements electronically to {nuance-at-northwestern.edu}
Web site: http://www.nuance.northwestern.edu/epic
Northwestern University is an equal opportunity, affirmative action employer. Members of historically underrepresented groups are strongly encouraged to apply.
==============================Original Headers============================== 22, 23 -- From jinsong-wu-at-northwestern.edu Mon Sep 17 10:02:10 2007 22, 23 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57]) 22, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8HF2A7B017821 22, 23 -- for {Microscopy-at-Microscopy.Com} ; Mon, 17 Sep 2007 10:02:10 -0500 22, 23 -- Received: from Jinsongwu (apache.ms.northwestern.edu [129.105.37.228]) 22, 23 -- by merle.it.northwestern.edu (Postfix) with SMTP id 017DD74E6 22, 23 -- for {Microscopy-at-Microscopy.Com} ; Mon, 17 Sep 2007 10:02:09 -0500 (CDT) 22, 23 -- Message-ID: {001f01c7f93b$b39f80b0$e4256981-at-Jinsongwu} 22, 23 -- Reply-To: "jinsong wu" {jinsong-wu-at-northwestern.edu} 22, 23 -- From: "jinsong wu" {jinsong-wu-at-northwestern.edu} 22, 23 -- To: {Microscopy-at-Microscopy.Com} 22, 23 -- Subject: SEM staff position avialable at Northwestern Univ. 22, 23 -- Date: Mon, 17 Sep 2007 10:02:01 -0500 22, 23 -- MIME-Version: 1.0 22, 23 -- Content-Type: text/plain; 22, 23 -- format=flowed; 22, 23 -- charset="iso-8859-1"; 22, 23 -- reply-type=original 22, 23 -- Content-Transfer-Encoding: 7bit 22, 23 -- X-Priority: 3 22, 23 -- X-MSMail-Priority: Normal 22, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 22, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 ==============================End of - Headers==============================
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Email: jim.c.russell-at-gmail.com Name: Jim Russell
Question: I have acquired a Cambridge 250 mk 2 SEM with LaB6 which appears complete with the notable exception of the operating maunal. Of course a service or maintenance manual would be extremely helpful too, so I am requesting help from the community. Does anyone have access to these manuals, or especially electronic versions? I thank you in advance, and our students thank you as well. Jim Russell
Dear All, Could you please share your experience/comments on usability of X-ray fluorescence as combined with SEM-EDS. The method seems promising and supplementary to EDS for analysis of heavy elements, but is not very popular, why?
Thanks in advance, Leszek
Dr. Leszek Kepinski Division of Nanomaterials Chemistry and Catalysis Institute of Low Temperature and Structure Research, Polish Academy of Sciences, P.O. Box 1410, 50-950 Wroclaw, Poland e-mail: L.Kepinski-at-int.pan.wroc.pl
==============================Original Headers============================== 2, 28 -- From L.Kepinski-at-int.pan.wroc.pl Tue Sep 18 08:15:14 2007 2, 28 -- Received: from mserv2.int.pan.wroc.pl (mserv2.int.pan.wroc.pl [156.17.85.6]) 2, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8IDFD03011293 2, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Sep 2007 08:15:14 -0500 2, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 2, 28 -- by mserv2.int.pan.wroc.pl (INTiBS (1.0)) with ESMTP id E0F9E134167 2, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Sep 2007 15:47:33 +0200 (CEST) 2, 28 -- Received: from mserv2.int.pan.wroc.pl ([127.0.0.1]) 2, 28 -- by localhost (mserv2.int.pan.wroc.pl [127.0.0.1]) (amavisd-new, port 10025) 2, 28 -- with LMTP id 09288-01-15 for {Microscopy-at-microscopy.com} ; 2, 28 -- Tue, 18 Sep 2007 15:47:31 +0200 (CEST) 2, 28 -- X-Virus-Scanner: This message was checked by NOD32 Antivirus system 2, 28 -- NOD32 for Linux Mail Server. 2, 28 -- For more information on NOD32 Antivirus System, 2, 28 -- please, visit our website: http://www.nod32.com/. 2, 28 -- Received: from [192.168.1.127] (gigax2.int.pan.wroc.pl [156.17.85.5]) 2, 28 -- by mserv2.int.pan.wroc.pl (INTiBS (1.0)) with ESMTP id 9666F134147 2, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Sep 2007 15:47:31 +0200 (CEST) 2, 28 -- Message-ID: {46EFCEE2.9050402-at-int.pan.wroc.pl} 2, 28 -- Date: Tue, 18 Sep 2007 15:13:06 +0200 2, 28 -- From: =?ISO-8859-2?Q?=22L=2E_K=EApi=F1ski=22?= {L.Kepinski-at-int.pan.wroc.pl} 2, 28 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 2, 28 -- MIME-Version: 1.0 2, 28 -- To: Microscopy-at-microscopy.com 2, 28 -- Subject: XRF in SEM-EDS 2, 28 -- Content-Type: text/plain; charset=ISO-8859-2; format=flowed 2, 28 -- Content-Transfer-Encoding: 7bit 2, 28 -- X-Virus-Scanned: by amavisd-new at int.pan.wroc.pl ==============================End of - Headers==============================
=} ZEISS EM109 transmission electron microscope with transfiber optics and external roll film camera
Colleagues, after switching from my routinely used 50 kV to 80 kV acceleration voltage I got some flash-overs / spark-overs but everything stabilized and worked fine. However, the transport motor of my photounit (roll film, trans fiber camera) started to run and want stop again, whatever I do (complete shut down; selective disconnection of photo unit, etc). I assume partial damage to one of the approx 40 chips (presumely a MOS chip) on the photo board due to a strike through from high voltage unit into the photo unit though they officially should be completely separated in electrical aspects. Has anybody experienced a similar situation and can give some advice? thanks, peter heimann
I have no idea how to embed an SDS-page gel, but given that it is polymerized one may expect it to sustain the treatments necessary for TEM observation. Only difference here: you don't have to care about water movement, you can dehydrate directly in pure ethanol. Perhaps the trickiest step would be impregnation (penetration of resin into the gel). Perhaps microwaving could help?
PLEASE, give us news about your results, I am sure it will interest some of us.
Good luck,
Stephane
----- Original Message ---- X-from: "k.levick-at-unsw.edu.au" {k.levick-at-unsw.edu.au} To: nizets2-at-yahoo.com Sent: Monday, September 17, 2007 8:31:50 AM
Hi everyone,
I have a sample of SDS-PAGE gel which I hope contains CdS nanoparticles attached to a phytochelatin. I would like to examine the particles in the TEM, but I am at a loss as to how to prepare them. Does anyone have any experience with extracting proteins from these gels? It has also been suggested that I treat the gel as a biological specimen (fix, dehydrate, embed, section). Does anyone have any tips they would be willing to share?
Thanks in advance,
Katie.
------------------------------- Katie Levick
Technical Officer Electron Microscope Unit UNSW Analytical Centre University of New South Wales Sydney NSW
02 9385 6390 k.levick-at-unsw.edu.au
==============================Original Headers============================== 9, 29 -- From k.levick-at-unsw.edu.au Mon Sep 17 01:21:28 2007 9, 29 -- Received: from smtp-dist.unsw.edu.au (smtp-dist-03.services.comms.unsw.EDU.AU [149.171.97.18]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8H6LQRb013671 9, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 17 Sep 2007 01:21:27 -0500 9, 29 -- Received: from localhost (avspam-01.services.comms.unsw.edu.au [149.171.100.16]) 9, 29 -- by smtp-dist.unsw.edu.au (8.13.6/8.13.6) with ESMTP id l8H6LQDT019989 9, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 17 Sep 2007 16:21:26 +1000 (EST) 9, 29 -- Received: from smtp.unsw.edu.au ([127.0.0.1]) 9, 29 -- by localhost (slag.comms.unsw.edu.au [127.0.0.1]) (amavisd-new, port 10025) 9, 29 -- with ESMTP id xqExRhLQPvi8 for {Microscopy-at-Microscopy.Com} ; 9, 29 -- Mon, 17 Sep 2007 16:21:25 +1000 (EST) 9, 29 -- Received: from AC026 ([129.94.150.26]) 9, 29 -- by smtp.unsw.edu.au (8.13.6/8.13.6) with SMTP id l8H6LPrp011995 9, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 17 Sep 2007 16:21:25 +1000 (EST) 9, 29 -- Message-ID: {008601c7f8f3$00be4780$1a965e81-at-AC026} 9, 29 -- From: "Katie Levick" {k.levick-at-unsw.edu.au} 9, 29 -- To: {Microscopy-at-Microscopy.Com} 9, 29 -- Subject: microscopy of SDS-page gel 9, 29 -- Date: Mon, 17 Sep 2007 16:21:37 +1000 9, 29 -- MIME-Version: 1.0 9, 29 -- Content-Type: text/plain; 9, 29 -- format=flowed; 9, 29 -- charset="iso-8859-1"; 9, 29 -- reply-type=original 9, 29 -- Content-Transfer-Encoding: 7bit 9, 29 -- X-Priority: 3 9, 29 -- X-MSMail-Priority: Normal 9, 29 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 9, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 ==============================End of - Headers==============================
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==============================Original Headers============================== 27, 21 -- From nizets2-at-yahoo.com Tue Sep 18 11:56:24 2007 27, 21 -- Received: from web37403.mail.mud.yahoo.com (web37403.mail.mud.yahoo.com [209.191.91.135]) 27, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l8IGuNqK008109 27, 21 -- for {microscopy-at-microscopy.com} ; Tue, 18 Sep 2007 11:56:23 -0500 27, 21 -- Received: (qmail 89912 invoked by uid 60001); 18 Sep 2007 16:56:22 -0000 27, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 27, 21 -- s=s1024; d=yahoo.com; 27, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 27, 21 -- b=07W1Y5AKkCNObAuev+Y3Yw1+NVrdGdeA4/kD5RV2VD63XrQrmSKe+OjWSfZ3U1kzCa/w6679cc3DYHl7aIuSAvit/rDtlvKPPS0ctPglDpJVi5vpebE2zfbetKKPG4WJ5qcY7T9f9Lq+A5BWWLVxHsvus7bBvim5MNVFf5b11Uw=; 27, 21 -- X-YMail-OSG: QEwastYVM1mZqN9H9chdb6IgH8tRYDF7d74rwFk_AYzuDRxIwpxE8WU1MjNq2S6F3qV0154f50nnlSKsUg0unGRBOchwqEkt1tC8gxrpgRmhdBD5S7FB7vIcOEa8uocOXk1ShhEPJyxzYxZc4zK2fHGp9aL3qQMWDoYLBtq8lsmy 27, 21 -- Received: from [209.191.88.134] by web37403.mail.mud.yahoo.com via HTTP; Tue, 18 Sep 2007 09:56:22 PDT 27, 21 -- X-Mailer: YahooMailRC/651.50 YahooMailWebService/0.7.134 27, 21 -- Date: Tue, 18 Sep 2007 09:56:22 -0700 (PDT) 27, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 27, 21 -- Subject: Re: [Microscopy] microscopy of SDS-page gel 27, 21 -- To: microscopy-at-microscopy.com 27, 21 -- MIME-Version: 1.0 27, 21 -- Content-Type: text/plain; charset=us-ascii 27, 21 -- Message-ID: {348563.89198.qm-at-web37403.mail.mud.yahoo.com} 27, 21 -- Content-Transfer-Encoding: 8bit 27, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l8IGuNqK008109 ==============================End of - Headers==============================
I ran samples by EDS on TEMs and SEMs, and also separately by WD-XRF on a Rigaku spectrometer. If I was not sure of the EDS results on a trace element and I had enough sample, I would press it on boric acid with a WC polished insert and run it by WD-XRF to confirm the element was there. WD-XRF is more sensitive, IMO, but takes much more sample than a TEM.
You might want to consider joining the XRF-L list and submitting your question to get a different point of view on WD-XRF versus EDS. I belong and it is free. It is fairly active but not as active as this list server. Here is the link.
I guess I better defend EDS on this list. I cut thin sections of anti-reflective coatings on lenses. The limit of EDS detection is usually taken to be 1% on the volume of material being analyzed. I was able to detect a TEM invisible layer in an anti-reflective layer multistack system that was only 10 angstoms thick. I got a half inch high peak by EDS on this anti-reflective stack layer. This was the smallest feature I was ever able to detect by EDS.
Paul Beauregard
At 08:16 AM 9/18/07 -0500, L.Kepinski-at-int.pan.wroc.pl wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 8, 26 -- From beaurega-at-westol.com Tue Sep 18 13:46:00 2007 8, 26 -- Received: from smtp-gateway-6.winbeam.com (smtp-gateway-6.winbeam.com [64.84.97.71]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8IIjwXn028807 8, 26 -- for {microscopy-at-microscopy.com} ; Tue, 18 Sep 2007 13:45:59 -0500 8, 26 -- X-Winbeam-MailScanner-Watermark: 1190745945.48513-at-crzaBCZpa6MX7uNHqdts+A 8, 26 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 8, 26 -- by smtp-gateway-6.winbeam.com (8.13.1/8.12.8) with SMTP id l8IIja1K018641 8, 26 -- for {microscopy-at-microscopy.com} ; Tue, 18 Sep 2007 14:45:37 -0400 8, 26 -- Received: (qmail 5743 invoked by uid 89); 18 Sep 2007 18:45:34 -0000 8, 26 -- Received: from pitts-69-72-12-99.dynamic-dialup.coretel.net (HELO beaurega) (69.72.12.99) 8, 26 -- by mail.winbeam.com with SMTP; 18 Sep 2007 18:45:34 -0000 8, 26 -- Message-Id: {3.0.6.32.20070918144533.007fc3e0-at-pop3.norton.antivirus} 8, 26 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus 8, 26 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 8, 26 -- Date: Tue, 18 Sep 2007 14:45:33 -0400 8, 26 -- To: L.Kepinski-at-int.pan.wroc.pl, microscopy-at-microscopy.com 8, 26 -- From: Beaurega {beaurega-at-westol.com} 8, 26 -- Subject: Re: [Microscopy] XRF in SEM-EDS 8, 26 -- In-Reply-To: {200709181316.l8IDGB2C012032-at-ns.microscopy.com} 8, 26 -- Mime-Version: 1.0 8, 26 -- Content-Type: text/plain; charset="us-ascii" 8, 26 -- X--MailScanner-Information: - Please contact Technical Support for more information 8, 26 -- X--MailScanner: Found to be clean (courtesy of MailScanner) 8, 26 -- X--MailScanner-SpamCheck: not spam (whitelisted), 8, 26 -- SpamAssassin (Disabled due to 10 consecutive timeouts) 8, 26 -- X--MailScanner-From: beaurega-at-westol.com ==============================End of - Headers==============================
I have only seen one other reply, so I will offer my comments.
I use SEM-EDS extensively. I have not used XRF in the SEM, but I have read a little about it.
For me, the prime benefit of x-ray analysis in the SEM is to obtain an elemental analysis at a point. I understand the development of capillary optics has made it possible to analyze areas measured in microns across. However, that is not the same as being able to use e-beam steering in conjunction with an EDS system to probe volumes just a couple microns across. The user would always need to be aware of the exact size and placement of the excitation volume due to the x-ray beam.
XRF does offer the promise of better sensitivity due to lower background and plenty of excitation. The bremstrallung is not present, so the peaks rest on a much smaller background. I presume there is plenty of excitation so that the limiting factor becomes the throughput of the x-ray detector. Both factors serve to improve detection limits.
Frankly, we have not had applications that would justify the cost of such an addition. Depending on your applications, the cost might be justified.
Warren Straszheim Iowa State University
-----Original Message----- X-from: L.Kepinski-at-int.pan.wroc.pl [mailto:L.Kepinski-at-int.pan.wroc.pl] Sent: Tuesday, September 18, 2007 8:17 AM To: wesaia-at-iastate.edu
There are some early instrument offerings of XRF in the SEM on the market, for elemental analysis. I have found these methods to be immature technology and a few years off from really being a useful tool. One would think XRF in the SEM would be a natural considering the success it has had as a stand-alone technique in a benchtop unit. But, the positioning of the detector relative to the sample is time-consuming, difficult to repeat with sample changes, and overall (currently) not conducive to the type of environment SEM's offer - multiple operator, general ease-of-use, and the quick, semi-quant chemical capability available from EDS.
My vote is to stick with SEM/EDS for now - and wait out XRF in the SEM for a few years yet - there is a very good reason why only small companies are offering this technology and the big players, Oxford and EDAX, have not yet entered the game - the technology is not yet competitive and profitable.
My solution, get a good benchtop XRF (Horiba, Rigaku, EDAX, or other) and compare the spectra via SLICE, which reports to compare XRF to EDS spectra fairly well. I don't have specific experience with comparing the two techniques with SLICE, but it's reportedly possible.
Andy Fisher Materials and Microscopy Consultant Charleston, SC
----- Original Message ----- X-from: {wesaia-at-iastate.edu} To: {andyfisher4-at-bellsouth.net} Sent: Tuesday, September 18, 2007 7:18 PM
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A purely personal viewpoint and probably unwisely sticking my head above the parapet ....
I first saw XRF on an SEM ~30 years ago. It was an interesting implementation, using a slide with something like 6 targets, selected by the user to optimise sensitivity for elements of interest in his particular application.
It is one of those experimental techniques which seems to have a number of advantages yet is not attractive enough to to make it commercially viable. A particular problem is that it combines well established techniques in a different way - this disrupts established 'political' territories. The XRF people do 'spectroscopy' and live in one lab, the SEM people do 'images' and work in another lab ....
When something comes along which crosses traditional boundaries, a lot of people get very uncomfortable and defensive, sometimes with justification but I do wonder whether lab politics is taking priority over scientific enquiry? Funding mechanisms also cause problems, with nobody being prepared to support techniques that they feel should be funded by someody else ....
To get a 'new' instrumental method accepted, especially commercially, is both a huge investment and requires enormous luck. Benchtop SIMS and X-ray tomography seem to be struggling. Then there are He ion microscopes and nanoSIMs, neither of which have yet, in my opinion, made a major impact. On the other hand, STM and AFM exploded out of nowhere. The benchtop TEM/STEM appeared at an exhibition a few years ago ... and disappeared. I've recently seen a proposal for a FE-SEM which would fit in your hand ..... -- Larry Stoter
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==============================Original Headers============================== 7, 15 -- From larry-at-cymru666.plus.com Wed Sep 19 14:55:55 2007 7, 15 -- Received: from ptb-relay03.plus.net (ptb-relay03.plus.net [212.159.14.214]) 7, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8JJtspA003006 7, 15 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Sep 2007 14:55:55 -0500 7, 15 -- Received: from [87.113.70.187] (helo=[192.168.1.2]) 7, 15 -- by ptb-relay03.plus.net with esmtp (Exim) id 1IY5eL-0002kJ-5M; Wed, 19 Sep 2007 20:55:53 +0100 7, 15 -- Mime-Version: 1.0 7, 15 -- Message-Id: {p06240802c317277b197f-at-[192.168.1.2]} 7, 15 -- In-Reply-To: {200709181325.l8IDPvOk022567-at-ns.microscopy.com} 7, 15 -- References: {200709181325.l8IDPvOk022567-at-ns.microscopy.com} 7, 15 -- Date: Wed, 19 Sep 2007 20:55:28 +0100 7, 15 -- To: L.Kepinski-at-int.pan.wroc.pl, Microscopy-at-MSA.Microscopy.Com 7, 15 -- From: Larry Stoter {larry-at-cymru666.plus.com} 7, 15 -- Subject: Re: [Microscopy] XRF in SEM-EDS 7, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Could someone who knows about doing quantitative EDS using a TEM give me a hand?
I have a paper that says they looked at phytoplankton, whole cells, in a TEM and did EDS on the cells.
They report data like cell volumes of 0.14 um3 and things like carbon at 34 fg/cell. I guess the calculations could be right, but can you make much distinction at the fg level with EDS?
If you know this kind of stuff and could help me out, it would be great. I can send the whole article as a PDF if you need it.
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. Visit http://www.aidslifecycle.org for more information about the ride.
==============================Original Headers============================== 8, 17 -- From jmkrupp-at-ucsc.edu Wed Sep 19 18:00:31 2007 8, 17 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 8, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8JN0U5C018742 8, 17 -- for {microscopy-at-microscopy.com} ; Wed, 19 Sep 2007 18:00:31 -0500 8, 17 -- Received: from [128.114.125.2] (HELO ucsc.edu) 8, 17 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 8, 17 -- with ESMTPS id 23647018 for microscopy-at-microscopy.com; Wed, 19 Sep 2007 16:00:25 -0700 8, 17 -- Received: from [128.114.25.182] (account jmkrupp-at-ucsc.edu HELO [128.114.25.182]) 8, 17 -- by silver.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 8, 17 -- with ESMTPA id 150798194 for microscopy-at-microscopy.com; Wed, 19 Sep 2007 16:00:24 -0700 8, 17 -- Mime-Version: 1.0 8, 17 -- Message-Id: {p06230907c31758e4b889-at-[128.114.25.182]} 8, 17 -- Date: Wed, 19 Sep 2007 16:00:23 -0700 8, 17 -- To: microscopy-at-microscopy.com 8, 17 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 8, 17 -- Subject: Quant. EDS with TEM 8, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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Question: I am using the Promega DeadEnd fluorometric TUNEL kit to check PCD status in maize anther section. I was wondering if anyone who has used this kit on plant tissues can give me suggestion on where need to pay additional attention and what should do/should not do besides following the protocol provided by promega. Thanks in advance.
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Email: swtkeller-at-yahoo.com Name: Sandra Keller
Organization: TA/SICCO
Title-Subject: [Filtered] TEM- Dislocation density calculation in plan view and cross-section
Question: Hi: I have been asked to perform dislocation density calculations of a sample using cross-sectional and in plan view TEM. Can someone out there review the calculations to make sure I am doing it correctly? Thanks in advance, Sandra
University of Cambridge Department of Earth Sciences
Research Analyst - permanent post
To operate, instruct in the use of, maintain, and develop the electron probe microanalysis (EPMA) facility.
We are looking for a dynamic and enterprising person with a sound knowledge of electron microscopy and analysis along with a background in geological or materials science. The successful applicant will also be responsible for maintaining quality control of analytical output and will advise on interpreting results.
Knowledge of the Cameca SX100 or SX50 instruments would be a distinct advantage.
Salary £25,134 - £32,796.
Please send CV and e-mails of 2 referees to the Administrator, vivien-at-esc.cam.ac.uk by Friday 12 October, 2007. Department of Earth Sciences, Downing Street, Cambridge CB2 3EQ.
DETAILS:
The successful applicant will be responsible for operation of the Cameca SX100 electron microprobe (with EDS and associated equipment.). This is a major analytical research facility with users from all areas of the department, elsewhere in the university, and externally. Duties will include: • Responsibility for the day-to-day operation, calibration & routine maintenance of electron microprobe and associated equipment. Managing all aspects of the laboratory including user schedules. • Advising users on suitable analytical strategies to solve specific problems. • Providing assistance, training & supervision of researchers, students and visiting academics from diverse backgrounds in the successful use of the facilities. • Monitoring and maintaining quality control of analytical output and assist in the interpretation of analyses in a geologically meaningful way. Ensuring that analytical results and methods are archived. • Designing, developing and testing analytical strategies. • Playing a major role in marketing and promoting the microprobe both within and outside the University to help generate running costs from usage of the equipment.
Although our instrument is under service contract with Cameca, the candidate should have the ability to carry-out initial trouble-shooting of both instrumental and analytical problems able to make small repairs to the instrumentation.
The post affords the opportunity to become familiar with modern state-of-the-art equipment. The primary focus of the position is successful operation of the facility however collaborative or independent research is encouraged.
Minimum requirements: Either: Extensive experience of operating an electron microprobe or comparable instrument Or: An MSc or PhD in geosciences or related fields and a knowledge of instrumental geochemistry, mineral chemistry and optical mineralogy and the ability to interpret analyses in a geologically meaningful way.
The lab manager will often be working with students and others who may be unfamiliar with microprobes, so good communications skills are required.
Reasonable knowledge of PC systems to setup/install, upgrade and maintain specialist software. Mechanical aptitude
Highly desirable: Extensive experience of operating and maintaining a Cameca SX-100 or SX-50.
Preference will be given to candidates with extensive experience of wavelength dispersive x-ray spectrometry and a good understanding of both the theory and application of electron probe microanalysis & electron microscopy. Understanding of the interactions of x-rays with matter as applied to spectroscopic techniques and of the operation of vacuum systems, radiation detectors etc. used to operate and collect data from this type of instrument.
Skills will ideally include a sound understanding of the generation, measurement, and counting statistics of x-rays, as well as statistical data analysis.
The ideal applicant will have a knowledge of data processing, statistics and quality control applied to x-ray detection and measurement and experience with laboratory management.
-- AB78-at-ESC.CAM.AC.UK DEPARTMENT OF EARTH SCIENCES UNIVERSITY OF CAMBRIDGE Tel +44 1223 333469 DOWNING STREET +44 1223 333400 CAMBRIDGE FAX +44 1223 333450 CB2 3EQ
Manager of XRD discussion list: http://www.jiscmail.ac.uk/lists/xrd.html
VirtualWDS: For the synthesis of wavelength-dispersive electron probe spectra. http://www.esc.cam.ac.uk/astaff/buckley/VirtualWDS.html
==============================Original Headers============================== 27, 25 -- From ab78-at-esc.cam.ac.uk Thu Sep 20 09:16:41 2007 27, 25 -- Received: from mail.esc.cam.ac.uk (mail.esc.cam.ac.uk [131.111.41.10]) 27, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8KEGe3j009261 27, 25 -- for {microscopy-at-microscopy.com} ; Thu, 20 Sep 2007 09:16:41 -0500 27, 25 -- Received: from post.esc.cam.ac.uk ([131.111.41.5]) 27, 25 -- by mail.esc.cam.ac.uk with esmtps (TLSv1:AES256-SHA:256) 27, 25 -- (Exim 4.54) 27, 25 -- id 1IYMpc-0004CV-4Z 27, 25 -- for microscopy-at-microscopy.com; Thu, 20 Sep 2007 15:16:40 +0100 27, 25 -- Received: from andyb.esc.cam.ac.uk ([192.168.17.155]) 27, 25 -- by post.esc.cam.ac.uk with esmtpsa (TLSv1:AES256-SHA:256) 27, 25 -- (Exim 4.66) 27, 25 -- (envelope-from {ab78-at-esc.cam.ac.uk} ) 27, 25 -- id 1IYMpb-0007hk-J3 27, 25 -- for microscopy-at-microscopy.com; Thu, 20 Sep 2007 15:16:40 +0100 27, 25 -- Message-ID: {46F28033.20904-at-esc.cam.ac.uk} 27, 25 -- Date: Thu, 20 Sep 2007 15:14:11 +0100 27, 25 -- From: Andy Buckley {ab78-at-esc.cam.ac.uk} 27, 25 -- Reply-To: ab78-at-esc.cam.ac.uk 27, 25 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 27, 25 -- MIME-Version: 1.0 27, 25 -- To: microscopy-at-microscopy.com 27, 25 -- Subject: Vacancy for an EPMA Specialist - University of Cambridge 27, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed 27, 25 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Does anyone have a manual for a TAAB-Pyper knifemaker, mk ll? We have inherited one in almost pristine condition but without any accompanying documentation. Any information on the use and maintenance of this compact little knifemaker would be very much appreciated.
Thanks.
Richard
Richard Easingwood Otago Centre for Electron Microscopy c/- Department of Anatomy & Structural Biology University of Otago, Dunedin, New Zealand ph: 0064 3 479 7301 cell: 021 222 4759 fax: 0064 3 479 5086 http://ocem.otago.ac.nz
==============================Original Headers============================== 8, 21 -- From richard.easingwood-at-stonebow.otago.ac.nz Thu Sep 20 19:39:38 2007 8, 21 -- Received: from mailhub2.otago.ac.nz (mailhub2.otago.ac.nz [139.80.64.247]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8L0dbrn016454 8, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 20 Sep 2007 19:39:38 -0500 8, 21 -- Received: from galadriel.otago.ac.nz (galadriel.otago.ac.nz [139.80.64.213]) 8, 21 -- by mailhub2.otago.ac.nz (8.13.8/8.13.8) with ESMTP id l8L0dZon015360 8, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 21 Sep 2007 12:39:35 +1200 8, 21 -- Received: from richarde.otago.ac.nz ([139.80.34.37]) 8, 21 -- by galadriel.otago.ac.nz with esmtp (Exim 4.63) 8, 21 -- (envelope-from {richard.easingwood-at-stonebow.otago.ac.nz} ) 8, 21 -- id 1IYWUG-0001Wp-76 8, 21 -- for Microscopy-at-MSA.Microscopy.Com; Fri, 21 Sep 2007 12:35:16 +1200 8, 21 -- Mime-Version: 1.0 (Apple Message framework v752.2) 8, 21 -- Content-Transfer-Encoding: 7bit 8, 21 -- Message-Id: {D93A62F9-FA66-4FC2-84A9-6196C0E943EE-at-stonebow.otago.ac.nz} 8, 21 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 8, 21 -- To: Microscopy-at-MSA.Microscopy.Com 8, 21 -- From: Richard Easingwood {richard.easingwood-at-stonebow.otago.ac.nz} 8, 21 -- Subject: TAAB-Pyper knifemaker manual? 8, 21 -- Date: Fri, 21 Sep 2007 12:39:33 +1200 8, 21 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
I am looking for advice on HR TEM/STEM and dedicated STEM. Our University ( TCD, Dublin ) is in the process of evaluating HR TEM/STEM instruments for purchase later this year. Our knowledge base in this area is limited and in the past I have found the listserver to be a great resource for helpful advice so I am hoping that some of you will have the time to respond. We have worked with standard TEM's ( 120KV & 200KV ) and SEM's for many years. HR TEM/STEM is however much more complex and advice from experienced users is invaluable in our assessment. Probably the best approach is for anyone willing to contact me directly as the views will be personal and I do not want to add unnecessary traffic to the listserver. The views will be treated as confidential and for internal consumption. Thank you in advance.
Our needs:-
We are looking for a full analytical TEM/STEM or STEM, with EELS/EDX. There is a wish to be able to add aberration correctors at a later stage. The proposed samples are:-
DNA functionalised Carbon Nanotubes Diamond-like carbon Ge NanoWires (possibly functionalised) Quantum dots Interface requiring FIB preparation
The instruments being evaluated are:-
Jeol JEM-2100F JEM-2200F
Hitachi HD-2300 HF-3300
Carl Zeiss Libra 200FE
FEI Tecnai F20 Tecnai F30 Titan
If you use any of these instruments I would love to hear from you. We are interested in your views on the correct instrument to choose. Were there any problems with installation, stability, reliability and service ? How easy are they to use, particularly if the skill levels tend more towards novice rather than expert ? Were there are integration problems with EELS/EDX ? Are there any advantages or disadvantages of going for a 300KV as opposed to a 200KV ? TEM/STEM vs dedicated STEM ?
This Question was submitted to Ask-A-Microscopist by (shoukry_aak-at-yahoo.com) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, September 21, 2007 at 10:41:05 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both shoukry_aak-at-yahoo.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: shoukry_aak-at-yahoo.com Name: Ahmed Shoukry Amin
Organization: faculty of science ,zoology dept. Cairo university, Egypt
Education: Graduate College
Location: Cairo ,Egypt
Question: Any lens is supposedly capable of magnification of objects .Is the lens inside our eye, a "magnifying" lens like any human-made lenses? what is its power ;its NA and its resolution? do these numbers differ among animals?