The Midwest Microscopy and Microanalysis Society will hold its final meeting of 2007 on Friday, November 16th, at Baxter Corporate Headquarters in Deerfield, IL. The program and registration information can be found on our website:
www.midwestmicroscopy.org
The deadline for registration is next Friday, November 9th, so please register soon. We look forward to seeing you there!
Elaine Schumacher President, Midwest Microscopy and Microanalysis Society
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 7, 27 -- From eschumacher-at-mccrone.com Thu Nov 1 07:37:34 2007 7, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA1CbY4Z019451 7, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 1 Nov 2007 07:37:34 -0500 7, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 7, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 23AB71A800B 7, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 1 Nov 2007 06:37:34 -0600 (CST) 7, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 7, 27 -- by pgp.mccrone.com (PGP Universal service); 7, 27 -- Thu, 01 Nov 2007 06:37:34 -0600 7, 27 -- X-PGP-Universal: processed 7, 27 -- Content-class: urn:content-classes:message 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="US-ASCII" 7, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 27 -- Subject: Meeting Announcement: MMMS Symposium on Microscopy 7, 27 -- Date: Thu, 1 Nov 2007 07:37:28 -0500 7, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150DA64-at-MCCRONEMSG.tmg.mccrone.com} 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- Thread-Topic: Meeting Announcement: MMMS Symposium on Microscopy 7, 27 -- Thread-Index: Acgcg/aIADIx9iZIQVmPpbMHuYKRzw== 7, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 7, 27 -- To: {Microscopy-at-MSA.Microscopy.Com} 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lA1CbY4Z019451 ==============================End of - Headers==============================
I'm putting together the first set of labs for my high school advanced microscopy class, and I wanted to put out a request for suggestions on interesting samples to use with light microscopes. I've got the old standards like pond water, sand, bugs, paper and such, but I was wondering if anyone had suggestions of common things that require little to no prep that would also be interesting that someone might not think of.
Thanks,
Justin A. Kraft
==============================Original Headers============================== 3, 27 -- From kraftpiano-at-gmail.com Thu Nov 1 11:36:27 2007 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.185]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA1GaQqq006168 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 1 Nov 2007 11:36:26 -0500 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so502209rvb 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 01 Nov 2007 09:36:21 -0700 (PDT) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=XnPRTPoNVPQ6mLsJ2B2Pc5/GOeNlLpLCY5XmZeqimuI=; 3, 27 -- b=GcD1FfUZ/wzRAgFQQnP5yoeo9lgwDwhHkJWZdtTWu6J7VbdN/ki62LZWEsR7+ulk7pUQz8mtgqXarLOBOG76Ot5H7HbgQPdX6I+QXX9AHPGl9hJAIyvA3YOWjW10HJ7wfRo26g+rPFHUOEpQeyMsfLn0IChS445OOkJSC3ZlsvE= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- b=LQYC1SUtzTAFRC1uPczVW3LyTcK2kGQMHFeOiLh/dHNRuQymIi39b8bwIn5VTXsH1cdCXkz8wDNmE8kyMjEyEZIWb16lQv8ag6/rhr5nez/h18OfAZzyDhOeVlAfTbNS8KKrKsxgAX00Z2l1+xB6PB/f/b04k7vLV7xO/CaQyO0= 3, 27 -- Received: by 10.140.200.16 with SMTP id x16mr385130rvf.1193934981814; 3, 27 -- Thu, 01 Nov 2007 09:36:21 -0700 (PDT) 3, 27 -- Received: by 10.141.136.6 with HTTP; Thu, 1 Nov 2007 09:36:21 -0700 (PDT) 3, 27 -- Message-ID: {25e2b0d20711010936x16175350v56231ccbb97c6c33-at-mail.gmail.com} 3, 27 -- Date: Thu, 1 Nov 2007 11:36:21 -0500 3, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: Interesting samples that require little-no prep. 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
I have a Kevex Delta III system (Not including detector) that, after much research, I have found out I will not be able to get working. I don't have the special monitor or monitor cable. There is one disk with it for software, but I'm not sure of the condition. When this was donated to us, I was told that it had been fully checked out and was functioning. If anyone wants parts from it, or wants the whole thing, it's taking up too much space in my lab. I'm open to any offers or for trade.
Thanks,
Justin A. Kraft
==============================Original Headers============================== 3, 27 -- From kraftpiano-at-gmail.com Thu Nov 1 11:38:40 2007 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.187]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA1Gcc3K008511 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 1 Nov 2007 11:38:40 -0500 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so502807rvb 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 01 Nov 2007 09:38:33 -0700 (PDT) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=KrJTPJeAZijV2yjwS5jS+hx9HctNTe5u80QYtP7RXrY=; 3, 27 -- b=DBIaALe5wUTYMkibUh00YYnAzOpSmmOfWXaK+8biFVQ9G6W2osupPddNM2pd+FZiBtumQ3N0watqeOC+k/0hcvFOcSlTHJVL3rCEYOJGRYav981J1/3+l2tNt82hR9wlSXsR6pajlnV2I/ZEwC6jqvTns82m0iAxUE8paXW5xCs= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- b=GeIierTlMFf5Eoa3+rJ/tcBQoSw3I+kSAWrf2kXMLVdk5+zjZdHwoZo/cAxeVn9PqFUC5eD1WjaWgjJJi9aftVcPAcy/m4TI07f4t1oeazMOp6lFKYhw1f04fclSC4awkvg44y4vA4Gb9rb0/kzkw6iUNw1uhsnvU9pbob117pQ= 3, 27 -- Received: by 10.140.141.15 with SMTP id o15mr391817rvd.1193935113165; 3, 27 -- Thu, 01 Nov 2007 09:38:33 -0700 (PDT) 3, 27 -- Received: by 10.141.136.6 with HTTP; Thu, 1 Nov 2007 09:38:32 -0700 (PDT) 3, 27 -- Message-ID: {25e2b0d20711010938k3f1cce3dp92b99cb2d91ff599-at-mail.gmail.com} 3, 27 -- Date: Thu, 1 Nov 2007 11:38:32 -0500 3, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: Kevex Delta III system for offer. 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
I suggest diatomaceous earth. These are very cool little exoskeletons and the organisms are probably consistent with what you might teach in your course. You can get it at your local nursery. Alternatively, you can rinse away the organics of toothpaste to isolate the diatomaceous earth.
If you have access to a stereomicroscope, let them look at the quintessential junk food: corn chips. I was amazed to see corn meal floating in a sea of oil.
Lastly, you might take a look at spider webs. There is a lot of research on the webs as models for materials design.
Best of luck,
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Happiness equals reality minus expectations." Tom Magliozzi
kraftpiano-at-gma il.com To gary.m.brown-at-exxonmobil.com 11/01/07 11:39 cc AM Subject [Microscopy] Interesting samples Please respond that require little-no prep. to kraftpiano-at-gma il.com
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
I'm putting together the first set of labs for my high school advanced microscopy class, and I wanted to put out a request for suggestions on interesting samples to use with light microscopes. I've got the old standards like pond water, sand, bugs, paper and such, but I was wondering if anyone had suggestions of common things that require little to no prep that would also be interesting that someone might not think of.
Thanks,
Justin A. Kraft
==============================Original Headers============================== 3, 27 -- From kraftpiano-at-gmail.com Thu Nov 1 11:36:27 2007 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.185]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA1GaQqq006168 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 1 Nov 2007 11:36:26 -0500 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so502209rvb 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 01 Nov 2007 09:36:21 -0700 (PDT) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition;
Justin Kraft asked for suggestions on interesting samples to use with light microscopes. The samples he mentioned are good for transmitted light microscopes. If a reflected light microscope is available, then many additional (opaque) samples become interesting: -aluminum foil -coins -microprinted objects such as currency -and most objects in real life. regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] ============================================= ----- Original Message ----- From: kraftpiano-at-gmail.com To: donc-at-asmicro.com Sent: Thursday, November 01, 2007 12:41 PM Subject: [a] [Microscopy] Interesting samples that require little-no prep.
I'm putting together the first set of labs for my high school advanced microscopy class, and I wanted to put out a request for suggestions on interesting samples to use with light microscopes. I've got the old standards like pond water, sand, bugs, paper and such, but I was wondering if anyone had suggestions of common things that require little to no prep that would also be interesting that someone might not think of.
Thanks,
Justin A. Kraft
==============================Original Headers============================== 8, 24 -- From donc-at-asmicro.com Thu Nov 1 13:02:59 2007 8, 24 -- Received: from smtp109.sbc.mail.re2.yahoo.com (smtp109.sbc.mail.re2.yahoo.com [68.142.229.96]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lA1I2xnS012634 8, 24 -- for {microscopy-at-microscopy.com} ; Thu, 1 Nov 2007 13:02:59 -0500 8, 24 -- Received: (qmail 8240 invoked from network); 1 Nov 2007 18:02:58 -0000 8, 24 -- Received: from unknown (HELO asm15) (asmicro-at-sbcglobal.net-at-76.252.21.24 with login) 8, 24 -- by smtp109.sbc.mail.re2.yahoo.com with SMTP; 1 Nov 2007 18:02:58 -0000 8, 24 -- X-YMail-OSG: LEdVZNcVM1kh1MIbP7CCdPhZUc1inoC7Ly_Djb0asMWngP0coM25UBqoVqGCXwJ2Z0v3HUk9npq0UNp4_w8pA9a6G84vUJCbGvy2vzvDMA50gr74FoahvJIg0S__U1UaZazJBi5kledGX7s- 8, 24 -- Message-ID: {003001c81cb1$627a4af0$0302a8c0-at-asm15} 8, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 8, 24 -- To: {kraftpiano-at-gmail.com} , "Microscopy List" {microscopy-at-microscopy.com} 8, 24 -- References: {200711011641.lA1GfRQR015534-at-ns.microscopy.com} 8, 24 -- Subject: Re: [a] [Microscopy] Interesting samples that require little-no prep. 8, 24 -- Date: Thu, 1 Nov 2007 14:00:26 -0400 8, 24 -- MIME-Version: 1.0 8, 24 -- Content-Type: text/plain; 8, 24 -- format=flowed; 8, 24 -- charset="iso-8859-1"; 8, 24 -- reply-type=original 8, 24 -- Content-Transfer-Encoding: 7bit 8, 24 -- X-Priority: 3 8, 24 -- X-MSMail-Priority: Normal 8, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 8, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
If you have DIC or Phase contrast. Use a tooth pick to scrape some cells from the inside of a cheek, smear them on a slide, add a drop of water and observe. Shows living cells, variety of bacteria, occassionally other critters. Seal the slide with wax and it will stay viable for hours.
On 1 Nov 2007 at 12:37, kraftpiano-at-gmail.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I'm putting together the first set of labs for my high school advanced } microscopy class, and I wanted to put out a request for suggestions on } interesting samples to use with light microscopes. I've got the old } standards like pond water, sand, bugs, paper and such, but I was } wondering if anyone had suggestions of common things that require } little to no prep that would also be interesting that someone might } not think of. } } Thanks, } } Justin A. Kraft } } ==============================Original Headers============================== } 3, 27 -- From kraftpiano-at-gmail.com Thu Nov 1 11:36:27 2007 } 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.185]) } 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA1GaQqq006168 } 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 1 Nov 2007 11:36:26 -0500 } 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so502209rvb } 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 01 Nov 2007 09:36:21 -0700 (PDT) } 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 3, 27 -- d=gmail.com; s=beta; } 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 3, 27 -- bh=XnPRTPoNVPQ6mLsJ2B2Pc5/GOeNlLpLCY5XmZeqimuI=; } 3, 27 -- b=GcD1FfUZ/wzRAgFQQnP5yoeo9lgwDwhHkJWZdtTWu6J7VbdN/ki62LZWEsR7+ulk7pUQz8mtgqXarLOBOG76Ot5H7HbgQPdX6I+QXX9AHPGl9hJAIyvA3YOWjW10HJ7wfRo26g+rPFHUOEpQeyMsfLn0IChS445OOkJSC3ZlsvE= } 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 3, 27 -- d=gmail.com; s=beta; } 3, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 3, 27 -- b=LQYC1SUtzTAFRC1uPczVW3LyTcK2kGQMHFeOiLh/dHNRuQymIi39b8bwIn5VTXsH1cdCXkz8wDNmE8kyMjEyEZIWb16lQv8ag6/rhr5nez/h18OfAZzyDhOeVlAfTbNS8KKrKsxgAX00Z2l1+xB6PB/f/b04k7vLV7xO/CaQyO0= } 3, 27 -- Received: by 10.140.200.16 with SMTP id x16mr385130rvf.1193934981814; } 3, 27 -- Thu, 01 Nov 2007 09:36:21 -0700 (PDT) } 3, 27 -- Received: by 10.141.136.6 with HTTP; Thu, 1 Nov 2007 09:36:21 -0700 (PDT) } 3, 27 -- Message-ID: {25e2b0d20711010936x16175350v56231ccbb97c6c33-at-mail.gmail.com} } 3, 27 -- Date: Thu, 1 Nov 2007 11:36:21 -0500 } 3, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} } 3, 27 -- To: microscopy-at-microscopy.com } 3, 27 -- Subject: Interesting samples that require little-no prep. } 3, 27 -- MIME-Version: 1.0 } 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 } 3, 27 -- Content-Transfer-Encoding: 7bit } 3, 27 -- Content-Disposition: inline } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 7, 25 -- From edelmare-at-muohio.edu Thu Nov 1 14:10:19 2007 7, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA1JAI3R026575 7, 25 -- for {microscopy-at-Microscopy.com} ; Thu, 1 Nov 2007 14:10:18 -0500 7, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 7, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id lA1JAH5g028117; 7, 25 -- Thu, 1 Nov 2007 15:10:17 -0400 7, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 7, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id lA1JAHls030586; 7, 25 -- Thu, 1 Nov 2007 15:10:17 -0400 7, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 7, 25 -- To: "kraftpiano-at-gmail.com" {kraftpiano-at-gmail.com} 7, 25 -- Date: Thu, 01 Nov 2007 15:10:23 -0400 7, 25 -- MIME-Version: 1.0 7, 25 -- Subject: Re: [Microscopy] Interesting samples that require little-no prep. 7, 25 -- CC: microscopy-at-Microscopy.com 7, 25 -- Message-ID: {4729EC5F.2707.BD88E29-at-edelmare.muohio.edu} 7, 25 -- Priority: normal 7, 25 -- In-reply-to: {200711011637.lA1GbWhf007361-at-ns.microscopy.com} 7, 25 -- References: {200711011637.lA1GbWhf007361-at-ns.microscopy.com} 7, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 7, 25 -- Content-type: text/plain; charset=US-ASCII 7, 25 -- Content-transfer-encoding: 7BIT 7, 25 -- Content-description: Mail message body 7, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 ==============================End of - Headers==============================
Does anyone have an XBO-75 illuminator to fit the Nikon Diaphot TMD and Diaphot 200 series microscopes that they are willing to sell? This is the beige illuminator with the black cable connector to the power supply. I only need the lamp housing itself, but willing to take the power supply if necessary. Alternatively, does anyone have the wiring schematic for the lamp housing and cable?
Regards, Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
************************************************************************ ****** The box said "Requires WindowsXP or better", so I bought a Macintosh. ************************************************************************ ******
==============================Original Headers============================== 9, 24 -- From glenmac-at-u.washington.edu Thu Nov 1 14:39:37 2007 9, 24 -- Received: from mxout5.cac.washington.edu (mxout5.cac.washington.edu [140.142.32.135]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA1JdZGB006928 9, 24 -- for {microscopy-at-microscopy.com} ; Thu, 1 Nov 2007 14:39:37 -0500 9, 24 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.141] (may be forged)) 9, 24 -- by mxout5.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id lA1JdX9V027304 9, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 9, 24 -- for {microscopy-at-microscopy.com} ; Thu, 1 Nov 2007 12:39:33 -0700 9, 24 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) 9, 24 -- (authenticated authid=glenmac) 9, 24 -- by smtp.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id lA1JdXNJ021619 9, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 9, 24 -- for {microscopy-at-microscopy.com} ; Thu, 1 Nov 2007 12:39:33 -0700 9, 24 -- Mime-Version: 1.0 (Apple Message framework v752.2) 9, 24 -- Content-Transfer-Encoding: 7bit 9, 24 -- Message-Id: {E1CC3D42-F720-49E4-B86B-0A6C3E7E8B98-at-u.washington.edu} 9, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 9, 24 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} 9, 24 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 9, 24 -- Subject: Need Lamp Housing 9, 24 -- Date: Thu, 1 Nov 2007 12:39:29 -0700 9, 24 -- X-Mailer: Apple Mail (2.752.2) 9, 24 -- X-PMX-Version: 5.3.3.310218, Antispam-Engine: 2.5.2.313940, Antispam-Data: 2007.11.1.121544 9, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='BODY_SIZE_800_899 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
I have been put in charge of looking around at specs for a 200KV TEM for a group that looks at nanoparticles.
Its been a long time since I was in the market for a new TEM and when I was, I was mostly a simple 80KV biological guy. So, any advice on what to get, not specific vendors, more like general features and things to avoid would help a lot at this point.
I know this is pretty vague, so you don't have to tell me that. As an old time EM type, but mostly biological, I have a passing understanding of things like EDS, EELS, STEM, SAD, etc., but could use some practical advice from the materials side.
Thanks
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. Visit http://www.aidslifecycle.org for more information about the ride.
==============================Original Headers============================== 8, 20 -- From jmkrupp-at-ucsc.edu Thu Nov 1 18:07:04 2007 8, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA1N74Yn024185 8, 20 -- for {microscopy-at-microscopy.com} ; Thu, 1 Nov 2007 18:07:04 -0500 8, 20 -- Received: from [128.114.125.129] (HELO ucsc.edu) 8, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 8, 20 -- with ESMTPS id 25504189 for microscopy-at-microscopy.com; Thu, 01 Nov 2007 16:06:59 -0700 8, 20 -- Received: by email-prod-fe-2.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 8, 20 -- with PIPE id 14639551; Thu, 01 Nov 2007 16:06:59 -0700 8, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 8, 20 -- Received: from [128.114.25.182] (account jmkrupp-at-ucsc.edu HELO [128.114.25.182]) 8, 20 -- by email-prod-fe-2.ucsc.edu (CommuniGate Pro SMTP 5.1.11) 8, 20 -- with ESMTPA id 14639492 for microscopy-at-microscopy.com; Thu, 01 Nov 2007 16:06:54 -0700 8, 20 -- Mime-Version: 1.0 8, 20 -- Message-Id: {p06230908c3500affdb75-at-[128.114.25.182]} 8, 20 -- Date: Thu, 1 Nov 2007 16:06:52 -0700 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 8, 20 -- Subject: new TEM ideas 8, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Huisheng, Since there don't seem to be any replies, I'll give it a shot. Generally speaking, biological samples do not provide a flat polished surface. Therefore there is NO quantitative analysis, ONLY qualitative. The only real exception I can think of is if you're using an analytical TEM with thin sections rather than an SEM.
In terms of improving your qualitative signal, lower kVs often help, depending upon what elements you're looking for. Ideally, you want the beam kV to be 2 to 3 times the x-ray keV you are looking for. Since most heavier elements also have low energy lines (L & M), low kV doesn't necessarily preclude looking for heavier elements, although it's not without its own issues.
However, more details of what your friend is trying to accomplish would be helpful.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: huisheng.jiao-at-gmail.com [mailto:huisheng.jiao-at-gmail.com] Sent: Wednesday, October 31, 2007 4:55 AM To: kenconverse-at-qualityimages.biz
Dear All:
Does anyone have suggestions about how to get more accurate EDX quantitative analysis results on biology samples? A friend of mine asked about the pratical procudure, but I just have material science background. Thanks in advance.
Regards, Huisheng JIAO
==============================Original Headers============================== 3, 27 -- From huisheng.jiao-at-gmail.com Wed Oct 31 03:50:29 2007 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.191]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9V8oSRj026924 3, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 31 Oct 2007 03:50:28 -0500 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so76137rvb 3, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 31 Oct 2007 01:50:28 -0700 (PDT) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime -version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=p8oEm5YToZxrvB3/b06btXmq9sdYtaCLikwFD70qNCQ=; 3, 27 -- b=NUhFO3qDgyHSib1GMFExjO6qJ9FrvsI5y4p2NPx30W5wHvgzG/qvxKGIQi/o5PHhqwnoDXC4Aw UqulL0hQYvA44gPkCbi9g81Srm8bi/r/yLqZ77VrrVh+0fh5HnTtWifAfcfmRb/Wo12tyNdkHHr7 HnXLw5Wx2ifXiOaPc5zUs= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content -transfer-encoding:content-disposition; 3, 27 -- b=FLb3JmgVnxu17E+ujKW6ffGxro2q1+VYds1g2UBZMrBsEepgIzzWnXVQL9fuYQDwA2L3/jbXu3 Ss2J2DbY8+xdMzqz2+VyJEXgwUFdA50CtrvjtVSEURO82nsz0MbwO0siqpZwnPgxvVCslMv2uo4c oGbal99k8phVRZwnlb7C0= 3, 27 -- Received: by 10.114.149.2 with SMTP id w2mr1678128wad.1193820628179; 3, 27 -- Wed, 31 Oct 2007 01:50:28 -0700 (PDT) 3, 27 -- Received: by 10.114.52.3 with HTTP; Wed, 31 Oct 2007 01:50:28 -0700 (PDT) 3, 27 -- Message-ID: {ed8387f0710310150i4784303l71f61894b85f4d9a-at-mail.gmail.com} 3, 27 -- Date: Wed, 31 Oct 2007 08:50:28 +0000 3, 27 -- From: "Huisheng JIAO" {huisheng.jiao-at-gmail.com} 3, 27 -- To: Microscopy-at-microscopy.com 3, 27 -- Subject: EDX on Biology sample 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 20, 27 -- From kenconverse-at-qualityimages.biz Thu Nov 1 18:27:37 2007 20, 27 -- Received: from dpmailmta05.doteasy.com (dpmailmta05-31.doteasy.com [65.61.219.91]) 20, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA1NRa2I004193 20, 27 -- for {microscopy-at-msa.microscopy.com} ; Thu, 1 Nov 2007 18:27:36 -0500 20, 27 -- Received: from dpmail16.doteasy.com (unverified [192.168.101.16]) 20, 27 -- by dpmailmta05.doteasy.com (DEO) with ESMTP id 2799638-1814644 20, 27 -- for multiple; Thu, 01 Nov 2007 14:31:16 -0800 20, 27 -- Received: from cpe-72-227-100-25.maine.res.rr.com [72.227.100.25] by dpmail16.doteasy.com with SMTP; 20, 27 -- Thu, 1 Nov 2007 16:27:18 -0700 20, 27 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 20, 27 -- To: {huisheng.jiao-at-gmail.com} , 20, 27 -- "MSA Listserver" {Microscopy-at-msa.microscopy.com} 20, 27 -- Subject: RE: [Microscopy] EDX on Biology sample 20, 27 -- Date: Thu, 1 Nov 2007 19:27:13 -0400 20, 27 -- Message-ID: {000201c81cde$bc322720$6401a8c0-at-Ken} 20, 27 -- MIME-Version: 1.0 20, 27 -- Content-Type: text/plain; 20, 27 -- charset="us-ascii" 20, 27 -- X-Priority: 3 (Normal) 20, 27 -- X-MSMail-Priority: Normal 20, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 20, 27 -- Thread-Index: Acgbm7+xHyU/2TFjRw2Nla8LrK9UHgBQRfYg 20, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 20, 27 -- Importance: Normal 20, 27 -- In-Reply-To: {200710310855.l9V8t7Xu031348-at-ns.microscopy.com} 20, 27 -- Content-Transfer-Encoding: 8bit 20, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lA1NRa2I004193 ==============================End of - Headers==============================
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Question: Hello All, I really need to hear a discussion from the experts on the practicality of detecting the elemental content in plants and seeds. A plant scientist here uses conventional methods to determine elemental and mineral content of his plants, but would like to know if it is possible to use EDX to confirm these values, not to quantify, just qualify relative values. Is there a particular software package designed to detect or be more sensitive to the elemental content in plants...seeds, leaf, etc. He is particularly interested in FE, Mg, Mn, Zn.
I guess I didn't copy my reply to the list. Cheng Huang and colleagues have been doing quantitative EDX on biological samples for 20 or so years. This can be done if the sample is frozen, a flat surface planed using cryomicrotome, then very carefully subliming any ice off the sample once in the microscope (on cryostage), withdrawing back into the cryotransfer unit, coating, then doing EDX. You can quantitate with the sample far from the nosepiece, to reduce any remaining topographical artifacts, and if you use as standards the ion of interest frozen in a carbon slurry matching the biological material as closely as possible. To avoid interference from Au lines, Cheng et al usually coat with Al for light element analysis, which is done at 15 kV.
cheers, Rosemary
Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 61 (0)2-6246 5475 GPO Box 1600 fax. 61 (0)2-6246 5334 Canberra, ACT 2601 Australia
On 2/11/07 10:32 AM, "kenconverse-at-qualityimages.biz" {kenconverse-at-qualityimages.biz} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Huisheng, } Since there don't seem to be any replies, I'll give it a shot. Generally } speaking, biological samples do not provide a flat polished surface. } Therefore there is NO quantitative analysis, ONLY qualitative. The only } real exception I can think of is if you're using an analytical TEM with thin } sections rather than an SEM. } } In terms of improving your qualitative signal, lower kVs often help, } depending upon what elements you're looking for. Ideally, you want the beam } kV to be 2 to 3 times the x-ray keV you are looking for. Since most heavier } elements also have low energy lines (L & M), low kV doesn't necessarily } preclude looking for heavier elements, although it's not without its own } issues. } } However, more details of what your friend is trying to accomplish would be } helpful. } } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } } -----Original Message----- } X-from: huisheng.jiao-at-gmail.com [mailto:huisheng.jiao-at-gmail.com] } Sent: Wednesday, October 31, 2007 4:55 AM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] EDX on Biology sample } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All: } } Does anyone have suggestions about how to get more accurate EDX quantitative } analysis results on biology samples? A friend of mine asked about the } pratical procudure, but I just have material science background. Thanks in } advance. } } Regards, Huisheng JIAO } } ==============================Original Headers============================== } 3, 27 -- From huisheng.jiao-at-gmail.com Wed Oct 31 03:50:29 2007 3, 27 -- } Received: from rv-out-0910.google.com (rv-out-0910.google.com } [209.85.198.191]) } 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l9V8oSRj026924 } 3, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 31 Oct 2007 03:50:28 } -0500 } 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so76137rvb } 3, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 31 Oct 2007 01:50:28 } -0700 (PDT) } 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 3, 27 -- d=gmail.com; s=beta; } 3, 27 -- } h=domainkey-signature:received:received:message-id:date:from:to:subject:mime } -version:content-type:content-transfer-encoding:content-disposition; } 3, 27 -- bh=p8oEm5YToZxrvB3/b06btXmq9sdYtaCLikwFD70qNCQ=; } 3, 27 -- } b=NUhFO3qDgyHSib1GMFExjO6qJ9FrvsI5y4p2NPx30W5wHvgzG/qvxKGIQi/o5PHhqwnoDXC4Aw } UqulL0hQYvA44gPkCbi9g81Srm8bi/r/yLqZ77VrrVh+0fh5HnTtWifAfcfmRb/Wo12tyNdkHHr7 } HnXLw5Wx2ifXiOaPc5zUs= } 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 3, 27 -- d=gmail.com; s=beta; } 3, 27 -- } h=received:message-id:date:from:to:subject:mime-version:content-type:content } -transfer-encoding:content-disposition; } 3, 27 -- } b=FLb3JmgVnxu17E+ujKW6ffGxro2q1+VYds1g2UBZMrBsEepgIzzWnXVQL9fuYQDwA2L3/jbXu3 } Ss2J2DbY8+xdMzqz2+VyJEXgwUFdA50CtrvjtVSEURO82nsz0MbwO0siqpZwnPgxvVCslMv2uo4c } oGbal99k8phVRZwnlb7C0= } 3, 27 -- Received: by 10.114.149.2 with SMTP id } w2mr1678128wad.1193820628179; } 3, 27 -- Wed, 31 Oct 2007 01:50:28 -0700 (PDT) } 3, 27 -- Received: by 10.114.52.3 with HTTP; Wed, 31 Oct 2007 01:50:28 -0700 } (PDT) 3, 27 -- Message-ID: } {ed8387f0710310150i4784303l71f61894b85f4d9a-at-mail.gmail.com} } 3, 27 -- Date: Wed, 31 Oct 2007 08:50:28 +0000 } 3, 27 -- From: "Huisheng JIAO" {huisheng.jiao-at-gmail.com} } 3, 27 -- To: Microscopy-at-microscopy.com } 3, 27 -- Subject: EDX on Biology sample } 3, 27 -- MIME-Version: 1.0 } 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 } 3, 27 -- Content-Transfer-Encoding: 7bit } 3, 27 -- Content-Disposition: inline ==============================End of - } Headers============================== } } } } } } ==============================Original Headers============================== } 20, 27 -- From kenconverse-at-qualityimages.biz Thu Nov 1 18:27:37 2007 } 20, 27 -- Received: from dpmailmta05.doteasy.com (dpmailmta05-31.doteasy.com } [65.61.219.91]) } 20, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } lA1NRa2I004193 } 20, 27 -- for {microscopy-at-msa.microscopy.com} ; Thu, 1 Nov 2007 18:27:36 -0500 } 20, 27 -- Received: from dpmail16.doteasy.com (unverified [192.168.101.16]) } 20, 27 -- by dpmailmta05.doteasy.com (DEO) with ESMTP id 2799638-1814644 } 20, 27 -- for multiple; Thu, 01 Nov 2007 14:31:16 -0800 } 20, 27 -- Received: from cpe-72-227-100-25.maine.res.rr.com [72.227.100.25] by } dpmail16.doteasy.com with SMTP; } 20, 27 -- Thu, 1 Nov 2007 16:27:18 -0700 } 20, 27 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } 20, 27 -- To: {huisheng.jiao-at-gmail.com} , } 20, 27 -- "MSA Listserver" {Microscopy-at-msa.microscopy.com} } 20, 27 -- Subject: RE: [Microscopy] EDX on Biology sample } 20, 27 -- Date: Thu, 1 Nov 2007 19:27:13 -0400 } 20, 27 -- Message-ID: {000201c81cde$bc322720$6401a8c0-at-Ken} } 20, 27 -- MIME-Version: 1.0 } 20, 27 -- Content-Type: text/plain; } 20, 27 -- charset="us-ascii" } 20, 27 -- X-Priority: 3 (Normal) } 20, 27 -- X-MSMail-Priority: Normal } 20, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 } 20, 27 -- Thread-Index: Acgbm7+xHyU/2TFjRw2Nla8LrK9UHgBQRfYg } 20, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 } 20, 27 -- Importance: Normal } 20, 27 -- In-Reply-To: {200710310855.l9V8t7Xu031348-at-ns.microscopy.com} } 20, 27 -- Content-Transfer-Encoding: 8bit } 20, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id lA1NRa2I004193 } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 33 -- From prvs=Rosemary.White=819bf644d-at-csiro.au Thu Nov 1 22:10:02 2007 7, 33 -- Received: from vic-MTAout1.csiro.au (vic-MTAout1.csiro.au [150.229.64.37]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA239xh5032707 7, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Nov 2007 22:10:00 -0500 7, 33 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; 7, 33 -- h=X-IronPort-AV:Received:Received:Received:Received: 7, 33 -- User-Agent:Date:Subject:From:To:Message-ID: 7, 33 -- In-Reply-To:Mime-version:Content-type: 7, 33 -- Content-transfer-encoding:Return-Path: 7, 33 -- X-OriginalArrivalTime; 7, 33 -- b=sJqeU7gvJS3zmf4K22Vz0cmm0sgr1tvoJbeUewp4M8AlOLFzdT41z 7, 33 -- DPGGG6/SHH3lxsKQIdrWbPGW7Bxsl5OY55Cyn1zKR8KoxhU3sYeqB 7, 33 -- nAjGS2TQANAOiOIvxE6Eki; 7, 33 -- X-IronPort-AV: E=Sophos;i="4.21,361,1188741600"; 7, 33 -- d="scan'208";a="151643553" 7, 33 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) 7, 33 -- by vic-ironport-int.csiro.au with ESMTP; 02 Nov 2007 14:09:57 +1100 7, 33 -- Received: from EXACTN1-CBR.nexus.csiro.au ([152.83.131.131]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 7, 33 -- Fri, 2 Nov 2007 14:09:57 +1100 7, 33 -- Received: OWA.CSIRO.AU 152.83.131.131 from 138.194.3.58 138.194.3.58 via HTTP with MS-WebStorage 6.0.6249 7, 33 -- Received: 138.194.3.58 138.194.3.58 from via HTTP with MS-WebStorage 6.0.6249 7, 33 -- User-Agent: Microsoft-Entourage/11.0.0.040405 7, 33 -- Date: Fri, 02 Nov 2007 14:14:11 +1100 7, 33 -- Subject: Re: [Microscopy] RE: EDX on Biology sample 7, 33 -- From: Rosemary White {Rosemary.White-at-csiro.au} 7, 33 -- To: {kenconverse-at-qualityimages.biz} , {Microscopy-at-microscopy.com} 7, 33 -- Message-ID: {C350E133.6F91%Rosemary.White-at-csiro.au} 7, 33 -- In-Reply-To: {200711012332.lA1NWmOs014547-at-ns.microscopy.com} 7, 33 -- Mime-version: 1.0 7, 33 -- Content-type: text/plain; 7, 33 -- charset="US-ASCII" 7, 33 -- Content-transfer-encoding: 7bit 7, 33 -- X-OriginalArrivalTime: 02 Nov 2007 03:09:57.0231 (UTC) FILETIME=[D8F52FF0:01C81CFD] ==============================End of - Headers==============================
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Email: ex42cdorm-at-Yahoo.co.uk Name: Maurice Vaughan
Organization: Beekeeping association
Title-Subject: [Filtered] Pollen grains
Question: Is there a way in which it is possible to view a pollen grain and observe the whole of the grain? When I focus on a grain and change the magnification slightly I get a blurred image and a good image of another part of the grain. I use 400X for pollen grains and also 100X (oil immersion) for photographic work. Is it possible to view the grain as a whole grain instead of a partial view? Thank you for your advice. Best regards, Mo.
On Nov 1, 2007, at 6:12 PM, ewestbrook-at-vsu.edu wrote:
} I really need to hear a discussion from the experts on the } practicality of detecting the elemental content in plants and } seeds. A plant scientist here uses conventional methods to } determine elemental and mineral content of his plants, but would } like to know if it is possible to use EDX to confirm these values, } not to quantify, just qualify relative values. Is there a } particular software package designed to detect or be more sensitive } to the elemental content in plants...seeds, leaf, etc. He is } particularly interested in FE, Mg, Mn, Zn. } } Your suggestions would be greatly appreciated.
Dear Winnie, Many years ago I did EDX on T pallidosa pollen and on germinating pine seeds. I was able to see differences in composition at different points in the specimen and at different stages in the case of the pine seeds. It definitely is possible--even straight-forward-- to take EDX spectra of plant materials with properly prepared specimens using either SEM or TEM. Whether one can detect the elements you list, however, depends on their concentration in the specimens. EDX is sensitive only to elements that constitute a reasonably large fraction of 1% or more of the specimen. (The exact sensitivity depends on the element, whether there are interferences from nearby peaks, the microscope parameters, and other factors.) I would be surprised if the elements you list are present in sufficient concentrations in a ~1 um^2 area of a section or ~1 um^3 volume that would be examined in TEM or SEM respectively, but I don't know enough about plant compositions to be authoritative about this. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 5, 22 -- From tivol-at-caltech.edu Fri Nov 2 11:45:25 2007 5, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA2GjOpT011874 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 2 Nov 2007 11:45:24 -0500 5, 22 -- Received: from water-dog.its.caltech.edu (water-dog [192.168.1.26]) 5, 22 -- by earth-ox-postvirus (Postfix) with ESMTP id 38B461BC4D; 5, 22 -- Fri, 2 Nov 2007 09:45:23 -0700 (PDT) 5, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 5, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id 35A351BA64; 5, 22 -- Fri, 2 Nov 2007 09:45:20 -0700 (PDT) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v752.3) 5, 22 -- In-Reply-To: {200711020112.lA21Ce58018614-at-ns.microscopy.com} 5, 22 -- References: {200711020112.lA21Ce58018614-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {45BCEEE9-4298-4F36-86EC-5CA589F6E4B4-at-caltech.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 22 -- Subject: Re: [Microscopy] viaWWW: EDX plants and seeds 5, 22 -- Date: Fri, 2 Nov 2007 09:45:33 -0700 5, 22 -- To: ewestbrook-at-vsu.edu, microscopy-at-msa.microscopy.com 5, 22 -- X-Mailer: Apple Mail (2.752.3) 5, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.4.5 ==============================End of - Headers==============================
Good Day: We hope to do tomography on 0.5 micron thick cryo samples. I am wondering how much, in terms of resolution, we would gain using energy filtering over using the small 10 or 20 micron objective aperture. Is there any way the diffraction aperture could be brought in to add resolution? The TEM platform is a Tecnai F20. Thanks bob
Guelph Regional Integrated Imaging Facility (GRIIF) Transmission Electron Microscope Facility Dept. of Molecular and Cell Biology New Science Complex, 488 Gordon St. University of Guelph Guelph Ontario, Canada, N1G 2W1 Phone: 519-824-4120 X 56409 Fax: 519-837-1802
==============================Original Headers============================== 5, 26 -- From bharris-at-uoguelph.ca Fri Nov 2 12:34:24 2007 5, 26 -- Received: from dargo.cs.uoguelph.ca (dargo.cs.uoguelph.ca [131.104.94.197]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA2HYOdd025131 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 2 Nov 2007 12:34:24 -0500 5, 26 -- Received: from aragorn.cs.uoguelph.ca (css.webmail.uoguelph.ca [131.104.93.20]) 5, 26 -- by dargo.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id lA2HYMip031018 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 2 Nov 2007 13:34:22 -0400 5, 26 -- Received: from webmail.uoguelph.ca (localhost.localdomain [127.0.0.1]) 5, 26 -- by aragorn.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id lA2HYMdE028901 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 2 Nov 2007 13:34:22 -0400 5, 26 -- Received: from 131.104.190.174 ([131.104.190.174]) by webmail.uoguelph.ca 5, 26 -- (Horde MIME library) with HTTP; Fri, 02 Nov 2007 13:34:22 -0400 5, 26 -- Message-ID: {20071102133422.u7f2d528g80g4o08-at-webmail.uoguelph.ca} 5, 26 -- Date: Fri, 02 Nov 2007 13:34:22 -0400 5, 26 -- From: Robert J Harris {bharris-at-uoguelph.ca} 5, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 26 -- Subject: TEM: Objective aperture vs EFTEM 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; 5, 26 -- charset=ISO-8859-1; 5, 26 -- DelSp="Yes"; 5, 26 -- format="flowed" 5, 26 -- Content-Disposition: inline 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) 5, 26 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.197 ==============================End of - Headers==============================
(1) Confocal microscopy. Pollen is one of the typical samples used to demo optical sectioning and 3D reconstruction.
(2) You could investigate the "Extended Focus" software systems. There are few different commercial programs for this. And there is a least one plugin for Image J (Freeware).
On 2 Nov 2007 at 9:57, ex42cdorm-at-Yahoo.co.uk wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both ex42cdorm-at-Yahoo.co.uk as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: ex42cdorm-at-Yahoo.co.uk } Name: Maurice Vaughan } } Organization: Beekeeping association } } Title-Subject: [Filtered] Pollen grains } } Question: Is there a way in which it is possible to view a pollen grain and observe the whole of the grain? } When I focus on a grain and change the magnification slightly I get a blurred image and a good image of another part of the grain. I use 400X for pollen grains and also 100X (oil immersion) for photographic work. } Is it possible to view the grain as a whole grain instead of a partial view? } Thank you for your advice. } Best regards, } Mo. } } Login Host: 172.141.238.212 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Fri Nov 2 08:55:57 2007 } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA2Dtts7027580 } 6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 2 Nov 2007 08:55:56 -0500 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240801c350dcddb602-at-[206.69.208.22]} } 6, 11 -- Date: Fri, 2 Nov 2007 08:55:54 -0500 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: ex42cdorm-at-Yahoo.co.uk (by way of MicroscopyListserver) } 6, 11 -- Subject: viaWWW: Pollen grains } 6, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 11, 24 -- From edelmare-at-muohio.edu Fri Nov 2 13:35:29 2007 11, 24 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA2IZT7j006119 11, 24 -- for {microscopy-at-Microscopy.com} ; Fri, 2 Nov 2007 13:35:29 -0500 11, 24 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 11, 24 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id lA2IZSRK025834 11, 24 -- for {microscopy-at-Microscopy.com} ; Fri, 2 Nov 2007 14:35:28 -0400 11, 24 -- Received: from [192.168.1.23] ([134.53.14.105]) 11, 24 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id lA2IZSBv023108 11, 24 -- for {microscopy-at-Microscopy.com} ; Fri, 2 Nov 2007 14:35:28 -0400 11, 24 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 11, 24 -- To: microscopy-at-Microscopy.com 11, 24 -- Date: Fri, 02 Nov 2007 14:35:29 -0400 11, 24 -- MIME-Version: 1.0 11, 24 -- Subject: Re: [Microscopy] viaWWW: Pollen grains 11, 24 -- Message-ID: {472B35B1.26681.10DECC3F-at-edelmare.muohio.edu} 11, 24 -- Priority: normal 11, 24 -- In-reply-to: {200711021357.lA2DvNWq028770-at-ns.microscopy.com} 11, 24 -- References: {200711021357.lA2DvNWq028770-at-ns.microscopy.com} 11, 24 -- X-mailer: Pegasus Mail for Windows (4.41) 11, 24 -- Content-type: text/plain; charset=US-ASCII 11, 24 -- Content-transfer-encoding: 7BIT 11, 24 -- Content-description: Mail message body 11, 24 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
On Nov 2, 2007, at 10:34 AM, bharris-at-uoguelph.ca wrote:
} Good Day: We hope to do tomography on 0.5 micron thick cryo samples. } I am wondering how much, in terms of resolution, we would gain using } energy filtering over using the small 10 or 20 micron objective } aperture. Is there any way the diffraction aperture could be brought } in to add resolution? The TEM platform is a Tecnai F20. Thanks bob
Dear Bob, 0.5 um cryospecimens are pretty thick for a 300 kV instrument, so I'd be doubtful that you'd get good resolution at 200 kV. A small obj. ap. will increase contrast at the cost of resolution, but that may not be too much of a cost, if the resolution is poor anyway. The diffraction aperture is an area-selecting aperture in imaging mode, so it would be of no benefit. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 5, 22 -- From tivol-at-caltech.edu Fri Nov 2 15:36:08 2007 5, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA2Ka837021057 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 2 Nov 2007 15:36:08 -0500 5, 22 -- Received: from water-dog.its.caltech.edu (water-dog [192.168.1.26]) 5, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id 3CB7E13C7D 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 2 Nov 2007 13:36:07 -0700 (PDT) 5, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 5, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id 3814C13D4C 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 2 Nov 2007 13:36:05 -0700 (PDT) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v752.3) 5, 22 -- In-Reply-To: {200711021734.lA2HYWn8025267-at-ns.microscopy.com} 5, 22 -- References: {200711021734.lA2HYWn8025267-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {DA46D6FE-82D8-432A-A4BD-16B816539881-at-caltech.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 22 -- Subject: Re: [Microscopy] TEM: Objective aperture vs EFTEM 5, 22 -- Date: Fri, 2 Nov 2007 13:36:18 -0700 5, 22 -- To: microscopy-at-msa.microscopy.com 5, 22 -- X-Mailer: Apple Mail (2.752.3) 5, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.4.5 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (acham002-at-fiu.edu) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, November 1, 2007 at 20:28:04 ---------------------------------------------------------------------------
Email: acham002-at-fiu.edu Name: Abdourahman Cham
Organization: Florida International University
Education: Graduate College
Location: Miami, Florida
Question: What are the relative costs of Microscopic analyses for STM, SEM, TEM and Optical Microscopes. I'm only interested in relative costs (ie TEM is more expensive than SEM for a given analysis) as opposed to absolute dollar amounts.
This Question was submitted to Ask-A-Microscopist by (carterj7-at-unlv.nevada.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, November 2, 2007 at 22:18:56 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both carterj7-at-unlv.nevada.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: carterj7-at-unlv.nevada.edu Name: Jonathan Carter
Organization: University of Nevada in Las Vegas
Education: Undergraduate College
Location: Las Vegas, NV
Title: Thermometry to determine fluid inclusion composition in mineral thin sections
Question: I am a geology undergraduate junior and am currently considering an undergraduate research project. I am curious if cooling and heating fluids in gypsum or carbonates can be done to tell if they are composed of Water, Co2, oil, or if this method would cause erroneous results due to the material's weakness?
Are there any other non-destructive approaches to determine the compositions of fluids trapped in fluid inclusions of carbonates?
I presume you are looking at fluid inclusions only.
1. Have you performed a quick literature search?
I think not. Even as a junior undergrad, please do your homework first, it's part of the learning process.
2. It can be done by interference microscopy. I have an old paper from the 70s from the folks at the USGS. (probably won't show up in a lit search)
3. Look for:
Burbankite, a (Sr, REE, Na, Ca)-carbonate in fluid inclusions from carbonatite-derived fluids; identification and characterization using laser Raman spectroscopy, SEM-EDX, and synchrotron micro-XRF analysis Bernhard Buehn, Andrew H. Rankin, Martin Radtke, Martin Haller, and Arndt Knoechel Buehn et al.
American Mineralogist.1999; 84: 1117-1125 (I have around somewhere as well)
4. For thermometry, look for:
See Hansen - Journal of Metamorphic Geology Volume 2 Issue 3 Page 249-264, September 1984
Abstract Fluid inclusion studies of rocks from the late Archaean amphibolite-facies to granulite-facies transition zone of southern India provide support for the hypothesis that CO2,-rich H2O-poor fluids were a major factor in the origin of the high-grade terrain. Charnockites, closely associated leucogranites and quartzo-feldspathic veins contain vast numbers of large CO2-rich inclusions in planar arrays in quartz and feldspar, whereas amphibole-bearing gray gneisses of essentially the same compositions as adjacent charnockites in mixed-facies quarries contain no large fluid inclusions. Inclusions in the northernmost incipient charnockites, as at Kabbal, Karnataka, occasionally contain about 25 mol. % of immiscible H2O lining cavity walls, whereas inclusions from the charnockite massif terrane farther south do not have visibile H2O
Microthermometry of CO2 inclusions shows that miscible CH4 and N2 must be small, probably less than 10mol.%combined. Densities of CO2 increase steadily from north to south across the transitional terrane. Entrapment pressures calculated from the CO2 equation of state range from 5 kbar in the north to 7.5 kbar in the south at the mineralogically inferred average metamorphic temperature of 750°C, in quantitative agreement with mineralogic geobarometry. This agreement leads to the inference that the fluid inclusions were trapped at or near peak metamorphic conditions.
Calculations on the stability of the charnockite assemblage biotite-orthopyroxene-K-feldspar-quartz show that an associated fluid phase must have less than 0.35 H2O activity at the inferred P and T conditions, which agrees with the petrographic observations. High TiO2 content of biotite stabilizes it to lower H2O activities, and the steady increase of biotite TiO2 southward in the area suggests progressive decrease of aH2O with increasing grade. Oxygen fugacities calculated from orthopyroxene-magnetite-quartz are considerably higher than the graphite CO2-O2 buffer, which explains the absence of graphite in the charnockites.
The present study quantifies the nature of the vapours in the southern India granulite metamorphism. It remains to be determined whether CO2-flushing of the crust can, by itself, create large terranes of largeion lithophile-depleted granulites, or whether removal of H2O-bearing anatectic melts is essential.
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
This message is from pH2. This message and any attachments may contain legally privileged or confidential information, and are intended only for the individual or entity identified above as the addressee. If you are not the addressee, or if this message has been addressed to you in error, you are not authorized to read, copy, or distribute this message and any attachments, and we ask that you please delete this message and attachments (including all copies) and notify the sender by return e-mail or by phone at 317-718-7020. Delivery of this message and any attachments to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or a privilege. All personal messages express views only of the sender, which are not to be attributed to pH2 and may not be copied or distributed without this statement.
-----Original Message----- X-from: carterj7-at-unlv.nevada.edu [mailto:carterj7-at-unlv.nevada.edu] Sent: Saturday, November 03, 2007 9:42 AM To: ph2-at-sprynet.com
This Question was submitted to Ask-A-Microscopist by (carterj7-at-unlv.nevada.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, November 2, 2007 at 22:18:56 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both carterj7-at-unlv.nevada.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: carterj7-at-unlv.nevada.edu Name: Jonathan Carter
Organization: University of Nevada in Las Vegas
Education: Undergraduate College
Location: Las Vegas, NV
Title: Thermometry to determine fluid inclusion composition in mineral thin sections
Question: I am a geology undergraduate junior and am currently considering an undergraduate research project. I am curious if cooling and heating fluids in gypsum or carbonates can be done to tell if they are composed of Water, Co2, oil, or if this method would cause erroneous results due to the material's weakness?
Are there any other non-destructive approaches to determine the compositions of fluids trapped in fluid inclusions of carbonates?
You can find that reference at {http://www.minsocam.org/MSA/AmMin/TOC/1999/JA99.html}
The full American Mineralogist from 1916 to present is online now.
Gordon Nord
On Nov 3, 2007, at 7:44 PM, ph2-at-sprynet.com wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } I presume you are looking at fluid inclusions only. } } 1. Have you performed a quick literature search? } } I think not. Even as a junior undergrad, please do your homework } first, it's part of the learning process. } } 2. It can be done by interference microscopy. I have an old paper } from } the 70s from the folks at the USGS. (probably won't show up in a } lit search) } } 3. Look for: } } Burbankite, a (Sr, REE, Na, Ca)-carbonate in fluid inclusions from } carbonatite-derived fluids; identification and characterization } using laser } Raman spectroscopy, SEM-EDX, and synchrotron micro-XRF analysis } Bernhard Buehn, Andrew H. Rankin, Martin Radtke, Martin Haller, and } Arndt } Knoechel } Buehn et al. } } American Mineralogist.1999; 84: 1117-1125 (I have around somewhere } as well) } } } 4. For thermometry, look for: } } See Hansen - Journal of Metamorphic Geology } Volume 2 Issue 3 Page 249-264, September 1984 } } } Abstract Fluid inclusion studies of rocks from the late Archaean } amphibolite-facies to granulite-facies transition zone of southern } India } provide support for the hypothesis that CO2,-rich H2O-poor fluids } were a } major factor in the origin of the high-grade terrain. Charnockites, } closely } associated leucogranites and quartzo-feldspathic veins contain vast } numbers } of large CO2-rich inclusions in planar arrays in quartz and feldspar, } whereas amphibole-bearing gray gneisses of essentially the same } compositions } as adjacent charnockites in mixed-facies quarries contain no large } fluid } inclusions. Inclusions in the northernmost incipient charnockites, } as at } Kabbal, Karnataka, occasionally contain about 25 mol. % of } immiscible H2O } lining cavity walls, whereas inclusions from the charnockite massif } terrane } farther south do not have visibile H2O } } Microthermometry of CO2 inclusions shows that miscible CH4 and N2 } must be } small, probably less than 10mol.%combined. Densities of CO2 increase } steadily from north to south across the transitional terrane. } Entrapment } pressures calculated from the CO2 equation of state range from 5 } kbar in the } north to 7.5 kbar in the south at the mineralogically inferred average } metamorphic temperature of 750°C, in quantitative agreement with } mineralogic } geobarometry. This agreement leads to the inference that the fluid } inclusions were trapped at or near peak metamorphic conditions. } } Calculations on the stability of the charnockite assemblage } biotite-orthopyroxene-K-feldspar-quartz show that an associated } fluid phase } must have less than 0.35 H2O activity at the inferred P and T } conditions, } which agrees with the petrographic observations. High TiO2 content of } biotite stabilizes it to lower H2O activities, and the steady } increase of } biotite TiO2 southward in the area suggests progressive decrease of } aH2O } with increasing grade. Oxygen fugacities calculated from } orthopyroxene-magnetite-quartz are considerably higher than the } graphite } CO2-O2 buffer, which explains the absence of graphite in the } charnockites. } } The present study quantifies the nature of the vapours in the } southern India } granulite metamorphism. It remains to be determined whether CO2- } flushing of } the crust can, by itself, create large terranes of largeion } lithophile-depleted granulites, or whether removal of H2O-bearing } anatectic } melts is essential. } } } ...................................................................... } Andrew Anthony "Tony" Havics, CHMM, CIH, PE } pH2, LLC } 5250 E US 36, Suite 830 } Avon, IN 46123 } (317) 718-7020 off } (317) 718-7038 fax } (317) 409-3238 cell } } 90% of Risk Management is knowing where to place the decimal } point...any } consultant can give you the other 10%(SM) } } This message is from pH2. 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All personal messages express views } only of } the sender, which are not to be attributed to pH2 and may not be } copied or } distributed without this statement. } } } -----Original Message----- } X-from: carterj7-at-unlv.nevada.edu [mailto:carterj7-at-unlv.nevada.edu] } Sent: Saturday, November 03, 2007 9:42 AM } To: ph2-at-sprynet.com } Subject: [Microscopy] AskAMicroscopist: Thermometry to determine fluid } inclusion } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } This Question was submitted to Ask-A-Microscopist by } (carterj7-at-unlv.nevada.edu) } from } http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A- } Microscopist.html on } Friday, November 2, 2007 at 22:18:56 } Remember to consider the Grade/Age of the student when considering the } Question } ---------------------------------------------------------------------- } ----- } Please reply to both carterj7-at-unlv.nevada.edu as well as to the } Microscopy Listserver } ---------------------------------------------------------------------- } ----- } } Email: carterj7-at-unlv.nevada.edu } Name: Jonathan Carter } } Organization: University of Nevada in Las Vegas } } Education: Undergraduate College } } Location: Las Vegas, NV } } Title: Thermometry to determine fluid inclusion composition } in mineral thin sections } } Question: I am a geology undergraduate junior and am currently } considering } an undergraduate research project. I am curious if cooling and heating } fluids in gypsum or carbonates can be done to tell if they are } composed of } Water, Co2, oil, or if this method would cause erroneous results } due to the } material's weakness? } } Are there any other non-destructive approaches to determine the } compositions } of fluids trapped in fluid inclusions of carbonates? } } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== } 9, 11 -- From zaluzec-at-ultra5.microscopy.com Sat Nov 3 08:32:15 2007 } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } lA3DWEwx024495 } 9, 11 -- for {microscopy-at-microscopy.com} ; Sat, 3 Nov 2007 08:32:15 } -0500 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240801c35228cd83c3-at-[206.69.208.22]} } 9, 11 -- Date: Sat, 3 Nov 2007 08:32:12 -0500 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: carterj7-at-unlv.nevada.edu (by way of Ask-A-Microscopist) } 9, 11 -- Subject: AskAMicroscopist: Thermometry to determine fluid } inclusion } 9, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 34, 28 -- From ph2-at-sprynet.com Sat Nov 3 18:43:06 2007 } 34, 28 -- Received: from elasmtp-scoter.atl.sa.earthlink.net } (elasmtp-scoter.atl.sa.earthlink.net [209.86.89.67]) } 34, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id lA3Nh6gP009011 } 34, 28 -- for {microscopy-at-microscopy.com} ; Sat, 3 Nov 2007 } 18:43:06 -0500 } 34, 28 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 34, 28 -- s=dk20050327; d=sprynet.com; } 34, 28 -- } b=WhvH7tA3kftyvttKpGhWQJiMBe5WByE2uo3z0YRfrYcdIQ1UhaiRaSxqEvKDLbwo; } 34, 28 -- h=Received:From:To:Cc:Subject:Date:MIME-Version:Content- } Type:Content-Transfer-Encoding:X-Mailer:In-reply-to:thread-index:X- } MimeOLE:Message-ID:X-ELNK-Trace:X-Originating-IP; } 34, 28 -- Received: from [68.77.95.59] (helo=user915fa8f284) } 34, 28 -- by elasmtp-scoter.atl.sa.earthlink.net with asmtp (Exim } 4.34) } 34, 28 -- id 1IoSdt-0007Xn-9l; Sat, 03 Nov 2007 19:43:05 -0400 } 34, 28 -- From: "Tony Havics" {ph2-at-sprynet.com} } 34, 28 -- To: {carterj7-at-unlv.nevada.edu} } 34, 28 -- Cc: "Micrscopy Listserve" {microscopy-at-microscopy.com} } 34, 28 -- Subject: RE: [Microscopy] AskAMicroscopist: Thermometry } to determine fluid inclusion } 34, 28 -- Date: Sat, 3 Nov 2007 19:42:51 -0400 } 34, 28 -- MIME-Version: 1.0 } 34, 28 -- Content-Type: text/plain; } 34, 28 -- charset="iso-8859-1" } 34, 28 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 } 34, 28 -- In-reply-to: {200711031342.lA3DgGxs007861-at-ns.microscopy.com} } 34, 28 -- thread-index: AcgeH1k8u1Bz6gehSverIhO/cLssUgAUrdkg } 34, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } 34, 28 -- Message-ID: {E1IoSdt-0007Xn-9l-at-elasmtp- } scoter.atl.sa.earthlink.net} } 34, 28 -- X-ELNK-Trace: } 6888e50b2be9b4fee5331016acda17f9a36bcf6c27442cf5bbd293d8105fea1e350bad } d9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c } 34, 28 -- X-Originating-IP: 68.77.95.59 } 34, 28 -- Content-Transfer-Encoding: 8bit } 34, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id lA3Nh6gP009011 } ==============================End of - } Headers==============================
==============================Original Headers============================== 7, 26 -- From gnord-at-mindspring.com Sun Nov 4 11:38:02 2007 7, 26 -- Received: from elasmtp-spurfowl.atl.sa.earthlink.net (elasmtp-spurfowl.atl.sa.earthlink.net [209.86.89.66]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA4Hc2Hx005031 7, 26 -- for {Microscopy-at-MSA.Microscopy.com} ; Sun, 4 Nov 2007 11:38:02 -0600 7, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 26 -- s=dk20050327; d=mindspring.com; 7, 26 -- b=cN/yIsNjHaJaICLwb/8vmnjWgiq0EShP8C6t+s8Gm/X4F7HA5AjSqScl/XZydz/A; 7, 26 -- h=Received:Mime-Version:In-Reply-To:References:Content-Type:Message-Id:Content-Transfer-Encoding:From:Subject:Date:To:X-Mailer:X-ELNK-Trace:X-Originating-IP; 7, 26 -- Received: from [72.86.44.214] (helo=[10.0.1.198]) 7, 26 -- by elasmtp-spurfowl.atl.sa.earthlink.net with asmtp (Exim 4.34) 7, 26 -- id 1IojQ9-0003Mk-AB 7, 26 -- for Microscopy-at-MSA.Microscopy.com; Sun, 04 Nov 2007 12:38:01 -0500 7, 26 -- Mime-Version: 1.0 (Apple Message framework v752.2) 7, 26 -- In-Reply-To: {200711032344.lA3Ni5Sg009691-at-ns.microscopy.com} 7, 26 -- References: {200711032344.lA3Ni5Sg009691-at-ns.microscopy.com} 7, 26 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 7, 26 -- Message-Id: {C8A42C04-1373-4578-B4ED-534086D1EDDC-at-mindspring.com} 7, 26 -- From: Gordon Nord {gnord-at-mindspring.com} 7, 26 -- Subject: RE: AskAMicroscopist: Thermometry to determine fluid inclusion 7, 26 -- Date: Sun, 4 Nov 2007 12:37:55 -0500 7, 26 -- To: Microscopy-at-MSA.Microscopy.com 7, 26 -- X-Mailer: Apple Mail (2.752.2) 7, 26 -- X-ELNK-Trace: 3df33169c923931ad4c20f6b8d69d888a4beb055f130b31ad91b4087751492361895afec583bce53350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 7, 26 -- X-Originating-IP: 72.86.44.214 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lA4Hc2Hx005031 ==============================End of - Headers==============================
The extended depth of field software, CombineZM, is freeware for Windows systems and can be found at http://www.hadleyweb.pwp.blueyonder.co.uk/CZM/combinezm.htm
I think this software is very easy to use and works well.
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
==============================Original Headers============================== 4, 25 -- From hanke-at-mee-inc.com Sun Nov 4 13:05:42 2007 4, 25 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38]) 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA4J5gMh019503 4, 25 -- for {microscopy-at-microscopy.com} ; Sun, 4 Nov 2007 13:05:42 -0600 4, 25 -- Received: (qmail 13460 invoked by uid 508); 4 Nov 2007 13:05:42 -0600 4, 25 -- Received: from 216.14.180.82 by mail.namisolutions.com (envelope-from {hanke-at-mee-inc.com} , uid 507) with qmail-scanner-1.24-st-qms 4, 25 -- (clamdscan: 0.87/4671. spamassassin: 3.0.2. perlscan: 1.24-st-qms. 4, 25 -- Clear:RC:1(216.14.180.82):. 4, 25 -- Processed in 0.047398 secs); 04 Nov 2007 19:05:42 -0000 4, 25 -- X-Antivirus-NAMISOLUTIONS-Mail-From: hanke-at-mee-inc.com via mail.namisolutions.com 4, 25 -- X-Antivirus-NAMISOLUTIONS: 1.24-st-qms (Clear:RC:1(216.14.180.82):. Processed in 0.047398 secs Process 13454) 4, 25 -- Received: from unknown (HELO ?192.168.1.4?) (216.14.180.82) 4, 25 -- by mail.namisolutions.com with SMTP; 4 Nov 2007 13:05:42 -0600 4, 25 -- Message-ID: {472E17FB.5030204-at-mee-inc.com} 4, 25 -- Date: Sun, 04 Nov 2007 13:05:31 -0600 4, 25 -- From: Larry Hanke {hanke-at-mee-inc.com} 4, 25 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716) 4, 25 -- X-Accept-Language: en-us, en 4, 25 -- MIME-Version: 1.0 4, 25 -- To: ex42cdorm-at-Yahoo.co.uk, microscopy-at-microscopy.com 4, 25 -- Subject: Re: viaWWW: Pollen grains 4, 25 -- References: {200711021357.lA2DvOMp028800-at-ns.microscopy.com} 4, 25 -- In-Reply-To: {200711021357.lA2DvOMp028800-at-ns.microscopy.com} 4, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The 35th annual meeting will be held on Wednesday November 14th 2007, West Park Conference Centre, 319 Perth Road, University of Dundee.
10.00 - 10.30 Registration and coffee Poster hanging and Trade Exhibition
Morning Session
Chair: Will Maxwell, Glasgow
10.30 - 11.15 David Lleres, University of Dundee, Scotland
Using Multiphoton Fluorescence lifetime Imaging Microscopy (FLIM) to measure Fluorescence Resonance Energy Transfer (FRET) to study protein-protein interactions in living cells
11.15 - 11.45 John Palero , University of Utrecht, Netherlands
Spectrally-Resolved Multiphoton Imaging of In Vivo and Excised Mouse Skin Tissues
11.45 - 12.00 One short talk from submitted abstracts
12.00-13.45 Lunch, Trade Exhibition and Posters
Afternoon Session
Chair: Richard Horobin, Glasgow
13.45 - 14.30 Paul Verkade, University of Bristol
Correlated light and electron microscopy (CLEM)
14.30 - 15.00 Neil Wilkinson, Gatan UK
New 3-D microscopy techniques in the SEM
15.15 - 15.45 Tea, Trade Exhibition and Posters
Chair: Laurence Tetley, Glasgow
15.45 - 16.30 Speaker to be confirmed
16.30 - 16.45 One short talk from submitted abstracts
16.45 - 16.55 Prize giving, Will Maxwell and Laurence Tetley
16.55 - 17.30 Drinks and Departure
For printable registration form, and other details of the meeting which includes a Trade Exhibition see :
A technician in our lab recently replaced the halogen lamp (epi-illumination) on our Nikon Eclipse E600, but we are no longer getting any illumination from it at the stage. He claims to have reattached the lamphouse in its previous location. We do have the lamp centering procedure for episcopic illumination in the Nikon manual but it seems to be fine adjustments (of about 6 different screws on the lamphouse) and these can’t be performed if you can’t see any illumination from the lamp after removing an eyepiece. We are reluctant to start making major adjustments of the screws without seeing any intensity. New bulb is working, all filters (e.g. ND4, ND8) are removed and field diaphragm is open along the Y-FL Epi-Fl attachment. We also have a double lamphouse adaptor for epi-illumination (with a separate mercury lamphouse attached on the other side), but this shouldn’t have been moved when changing the halogen bulb.
Am I (and the two others who’ve looked at it) missing something or is this supposed to be tricky? Anyone have tips as to how to get this properly realigned? I'm tempted to remove it myself since I have no conception of how the alignment works.
Thanks,
T. Kevin Croat
Senior Research Scientist
Physics Dept. and McDonnell Center for the Space Sciences
Washington University in St. Louis
tkc-at-wustl.edu
==============================Original Headers============================== 10, 25 -- From tkc-at-wustl.edu Mon Nov 5 11:11:10 2007 10, 25 -- Received: from physsmtp.wustl.edu (physsmtp.wustl.edu [128.252.125.7]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA5HBA80018009 10, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Nov 2007 11:11:10 -0600 10, 25 -- Received: from physsmtp.wustl.edu (localhost.localdomain [127.0.0.1]) 10, 25 -- by physsmtp.wustl.edu (Postfix) with ESMTP id 316855F43E5 10, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Nov 2007 11:11:07 -0600 (CST) 10, 25 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on physsmtp.wustl.edu 10, 25 -- X-Spam-Level: 10, 25 -- X-Spam-Status: No, score=-4.1 required=5.0 tests=ALL_TRUSTED,AWL,BAYES_00 10, 25 -- autolearn=ham version=3.1.8 10, 25 -- Received: from [128.252.35.132] (metastable.wustl.edu [128.252.35.132]) 10, 25 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 10, 25 -- (No client certificate requested) 10, 25 -- by physsmtp.wustl.edu (Postfix) with ESMTP id 1EC9A5F401E 10, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Nov 2007 11:11:07 -0600 (CST) 10, 25 -- Message-ID: {472F4EC8.10609-at-wustl.edu} 10, 25 -- Date: Mon, 05 Nov 2007 11:11:36 -0600 10, 25 -- From: Kevin Croat {tkc-at-wustl.edu} 10, 25 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 10, 25 -- MIME-Version: 1.0 10, 25 -- To: Microscopy-at-microscopy.com 10, 25 -- Subject: LM Nikon Eclipse epi-illumination alignment problem 10, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 25 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Hi Justin If you have a standard wide-field microscope without any special contrast options, hairs from different critters always make a good impression - dry hair stuck to a slide with tape or glue at the ends; use 10X to 25X objectives, ask - how are, say cat hairs different from a human hair. Look at specialized hairs - porcupine quills; cat whiskers; eyebrow and eyelash hair. You can easily see detail such as scales, root, medulla and cortex. Another simple dry prep that is very impressive - feathers. How do flight feathers differ from down feathers.
Rick,
Richard Harris Manager - Imaging and Data Systems Biotron Experimental Climate Change Research Facility University of Western Ontario, London Ontario, CANADA. N6A 5B7 Ph. 519-661-2111 ext. 86780 Fax 519-661-3935 e-mail rjharris-at-uwo.ca web: www.biotron.uwo.ca
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Thursday, November 01, 2007 12:47 PM To: rjharris-at-uwo.ca
I'm putting together the first set of labs for my high school advanced microscopy class, and I wanted to put out a request for suggestions on interesting samples to use with light microscopes. I've got the old standards like pond water, sand, bugs, paper and such, but I was wondering if anyone had suggestions of common things that require little to no prep that would also be interesting that someone might not think of.
Thanks,
Justin A. Kraft
==============================Original Headers============================== 3, 27 -- From kraftpiano-at-gmail.com Thu Nov 1 11:36:27 2007 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.185]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA1GaQqq006168 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 1 Nov 2007 11:36:26 -0500 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so502209rvb 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 01 Nov 2007 09:36:21 -0700 (PDT) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime -version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=XnPRTPoNVPQ6mLsJ2B2Pc5/GOeNlLpLCY5XmZeqimuI=; 3, 27 -- b=GcD1FfUZ/wzRAgFQQnP5yoeo9lgwDwhHkJWZdtTWu6J7VbdN/ki62LZWEsR7+ulk7pUQz8mtgq XarLOBOG76Ot5H7HbgQPdX6I+QXX9AHPGl9hJAIyvA3YOWjW10HJ7wfRo26g+rPFHUOEpQeyMsfL n0IChS445OOkJSC3ZlsvE= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content -transfer-encoding:content-disposition; 3, 27 -- b=LQYC1SUtzTAFRC1uPczVW3LyTcK2kGQMHFeOiLh/dHNRuQymIi39b8bwIn5VTXsH1cdCXkz8wD NmE8kyMjEyEZIWb16lQv8ag6/rhr5nez/h18OfAZzyDhOeVlAfTbNS8KKrKsxgAX00Z2l1+xB6PB /f/b04k7vLV7xO/CaQyO0= 3, 27 -- Received: by 10.140.200.16 with SMTP id x16mr385130rvf.1193934981814; 3, 27 -- Thu, 01 Nov 2007 09:36:21 -0700 (PDT) 3, 27 -- Received: by 10.141.136.6 with HTTP; Thu, 1 Nov 2007 09:36:21 -0700 (PDT) 3, 27 -- Message-ID: {25e2b0d20711010936x16175350v56231ccbb97c6c33-at-mail.gmail.com} 3, 27 -- Date: Thu, 1 Nov 2007 11:36:21 -0500 3, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: Interesting samples that require little-no prep. 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 12, 27 -- From rjharris-at-uwo.ca Mon Nov 5 11:21:34 2007 12, 27 -- Received: from uwo.ca (v320-147-lb.its.uwo.ca [129.100.74.147]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA5HLY20030231 12, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 5 Nov 2007 11:21:34 -0600 12, 27 -- MIME-version: 1.0 12, 27 -- Content-transfer-encoding: 7BIT 12, 27 -- Content-type: text/plain; charset=us-ascii 12, 27 -- Received: from zeppo.mail.uwo.pri (salk.mail.uwo.pri [172.29.32.41]) 12, 27 -- by zeppo.mail.uwo.pri 12, 27 -- (Sun Java(tm) System Messaging Server 6.3-3.01 (built Jul 12 2007; 32bit)) 12, 27 -- with ESMTP id {0JR1007S1MVY9540-at-zeppo.mail.uwo.pri} for 12, 27 -- Microscopy-at-MSA.Microscopy.Com; Mon, 05 Nov 2007 12:21:34 -0500 (EST) 12, 27 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17]) 12, 27 -- by zeppo.mail.uwo.pri 12, 27 -- (Sun Java(tm) System Messaging Server 6.3-3.01 (built Jul 12 2007; 32bit)) 12, 27 -- with ESMTPSA id {0JR100J04MVYUU20-at-zeppo.mail.uwo.pri} for 12, 27 -- Microscopy-at-MSA.Microscopy.Com; Mon, 05 Nov 2007 12:21:34 -0500 (EST) 12, 27 -- From: Richard Harris {rjharris-at-uwo.ca} 12, 27 -- To: kraftpiano-at-gmail.com, MSA Listserver {Microscopy-at-MSA.Microscopy.Com} 12, 27 -- References: {200711011647.lA1Gl5SY028933-at-ns.microscopy.com} 12, 27 -- In-reply-to: {200711011647.lA1Gl5SY028933-at-ns.microscopy.com} 12, 27 -- Subject: RE: [Microscopy] Interesting samples that require little-no prep. 12, 27 -- Date: Mon, 05 Nov 2007 12:21:35 -0500 12, 27 -- Message-id: {004401c81fd0$5195f5a0$f4c1e0e0$-at-ca} 12, 27 -- X-Mailer: Microsoft Office Outlook 12.0 12, 27 -- Content-language: en-us 12, 27 -- Thread-index: AcgcptX3PSaK8coDQemOXVUgL/XQ3wDKDGxg ==============================End of - Headers==============================
Do you mean the arc lamp (not halogen) system for epi-fluorescence? I have a write-up that is fairly complete for the E600 system. http://www.bio.umass.edu/microscopy/doc/ArcLampAlignment.pdf
If you don't have the alignment objective (very handy, usually comes with the scope, may be in your "accessories drawer"), just take an objective off and put a white piece of card on the stage to project the arc plasma/electrode images. I use the blue filters (eye is less sensitive and the green sensitivity and Hg 546 line are wicked bright), add some ND filters, and stop down the field diaphragm (acts as a CAD for the rear focal plane so you see the focus better).
If alignment is way off you may have to "walk" the controls to find the arc image. The key is to distinguish the forward image and reflected image; when you find an image, the reflector controls will ONLY move the reflected image. Use the illuminator collector lens focus to sharpen the forward image, and the controls that physically move the LAMP to set the forward position just to one side of the center and sharp. Then use the reflector controls to bring the reflected image just next to the forward image and focus the reflector to make that image as sharp as possible. Remember to open the field diaphragm if it was stopped down. This process puts the focussed image of the arc at the rear focus of the objective so that the illumination rays are completely defocussed and give even illumination.
Hope this helps.
Dale
tkc-at-wustl.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } A technician in our lab recently replaced the halogen lamp } (epi-illumination) on our Nikon Eclipse E600, but we are no longer } getting any illumination from it at the stage. He claims to have } reattached the lamphouse in its previous location. We do have the lamp } centering procedure for episcopic illumination in the Nikon manual but } it seems to be fine adjustments (of about 6 different screws on the } lamphouse) and these can’t be performed if you can’t see any } illumination from the lamp after removing an eyepiece. We are reluctant } to start making major adjustments of the screws without seeing any } intensity. New bulb is working, all filters (e.g. ND4, ND8) are removed } and field diaphragm is open along the Y-FL Epi-Fl attachment. We also } have a double lamphouse adaptor for epi-illumination (with a separate } mercury lamphouse attached on the other side), but this shouldn’t have } been moved when changing the halogen bulb. } } Am I (and the two others who’ve looked at it) missing something or is } this supposed to be tricky? Anyone have tips as to how to get this } properly realigned? I'm tempted to remove it myself since I have no } conception of how the alignment works. } } } Thanks, } } T. Kevin Croat } } Senior Research Scientist } } Physics Dept. and McDonnell Center for the Space Sciences } } Washington University in St. Louis } } tkc-at-wustl.edu } } } ==============================Original Headers============================== } 10, 25 -- From tkc-at-wustl.edu Mon Nov 5 11:11:10 2007 } 10, 25 -- Received: from physsmtp.wustl.edu (physsmtp.wustl.edu [128.252.125.7]) } 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA5HBA80018009 } 10, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Nov 2007 11:11:10 -0600 } 10, 25 -- Received: from physsmtp.wustl.edu (localhost.localdomain [127.0.0.1]) } 10, 25 -- by physsmtp.wustl.edu (Postfix) with ESMTP id 316855F43E5 } 10, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Nov 2007 11:11:07 -0600 (CST) } 10, 25 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on physsmtp.wustl.edu } 10, 25 -- X-Spam-Level: } 10, 25 -- X-Spam-Status: No, score=-4.1 required=5.0 tests=ALL_TRUSTED,AWL,BAYES_00 } 10, 25 -- autolearn=ham version=3.1.8 } 10, 25 -- Received: from [128.252.35.132] (metastable.wustl.edu [128.252.35.132]) } 10, 25 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) } 10, 25 -- (No client certificate requested) } 10, 25 -- by physsmtp.wustl.edu (Postfix) with ESMTP id 1EC9A5F401E } 10, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Nov 2007 11:11:07 -0600 (CST) } 10, 25 -- Message-ID: {472F4EC8.10609-at-wustl.edu} } 10, 25 -- Date: Mon, 05 Nov 2007 11:11:36 -0600 } 10, 25 -- From: Kevin Croat {tkc-at-wustl.edu} } 10, 25 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) } 10, 25 -- MIME-Version: 1.0 } 10, 25 -- To: Microscopy-at-microscopy.com } 10, 25 -- Subject: LM Nikon Eclipse epi-illumination alignment problem } 10, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 10, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 22 -- From dac-at-research.umass.edu Mon Nov 5 11:37:15 2007 9, 22 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA5HbFvX010182 9, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Nov 2007 11:37:15 -0600 9, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 9, 22 -- (authenticated bits=0) 9, 22 -- by race4.oit.umass.edu (8.14.1/8.14.1) with ESMTP id lA5HbEwn020186 9, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 9, 22 -- Mon, 5 Nov 2007 12:37:14 -0500 9, 22 -- Message-ID: {472FFDBC.6080005-at-research.umass.edu} 9, 22 -- Date: Tue, 06 Nov 2007 00:38:04 -0500 9, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 9, 22 -- Reply-To: dac-at-research.umass.edu 9, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.6) Gecko/20070802 SeaMonkey/1.1.4 9, 22 -- MIME-Version: 1.0 9, 22 -- To: tkc-at-wustl.edu, Microscopy Listserver {Microscopy-at-microscopy.com} 9, 22 -- Subject: Re: [Microscopy] LM Nikon Eclipse epi-illumination alignment problem 9, 22 -- References: {200711051717.lA5HHlWR027096-at-ns.microscopy.com} 9, 22 -- In-Reply-To: {200711051717.lA5HHlWR027096-at-ns.microscopy.com} 9, 22 -- Content-Type: text/plain; charset=windows-1252; format=flowed 9, 22 -- Content-Transfer-Encoding: 8bit 9, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Hitachi S4300 Photo CRT & Roll Film Camera. Should also suit other models of Hitachi SEM's. As far as I am aware it is perfect working order. It is free to a good home. Shipping to be arranged by "buyer". I hate to see it being wasted so hopefully it will be of use to one of you.
Best wishes,
Colin
Colin Reid Senior Experimental Officer, Centre for Microscopy and Analysis (CMA), East End 4, Trinity College Dublin, Dublin 2.
As we are in workings for our new TEM, we have to watch closely to avoid that the relative clean environnement we have found wont be crabed by a bad electrical wiring or something else like that.
So my question is on UPS. What about EM interferencies generated by an 10 kVA UPS. Is that a concern for HR TEM/STEM, HR-SEM, etc, or are the level too low, and the frequency generated too high to be a source of problem ? The spec I've read gives only caracteristics in a 150 kHz - 30 MHz, but nothing in the ~0 - 20 kHz.
Of coarse distance is the easiest way to limit the effects of possible interferencies radiated by the UPS itself, but we don't want to be on the safe side, and, for exemple, put the UPS too far away... and catch again interferencies from the environnement by the needed long cable.
What are the feedbacks form UPS users. Until now, we don't have UPS in use, on the EM equipements.
Thanks
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tonygr-at-mit.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tonygr-at-mit.edu Name: Tony Garratt-Reed
Organization: MIT
Title-Subject: [Filtered] New England Society for Macroscopy Fall Symposium
Question: The New England Society for Microscopy is pleased to announce that once again the annual Fall Symposium and Business Meeting of the society will be held at Gordon College, Wenham, MA. We will convene at 12:45 pm on Thursday December 13th. 2007 for technical sessions, to be followed in turn by the annual business meeting, dinner, and an after-dinner presentation.
Full details, including the program, registration information and travel directions may be found on the NESM web site at http://nesm.cims.harvard.edu
All people with any interest in any form of microscopy, as practicioners, vendors, or in any other capacity, are warmly invited to attend.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both przybylowicz-at-tlabs.ac.za as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: przybylowicz-at-tlabs.ac.za Name: Wojciech J. Przybylowicz
Organization: iThemba LABS
Title-Subject: [Filtered] post doc position
Question: Post-doctoral fellow (NMP) - two years contract with extension possibility
Position: Nuclear microprobe analysis of thin frozen-hydrated biological specimens
Applications are invited for this position in our Materials Research Group (MRG) of iThemba LABS, situated ca. 30 km from Cape Town, South Africa.
Responsibilities will include: - Adapting of cryo-stage coupled to the MRG nuclear microprobe for measurements of thin specimens in frozen-hydrated state
- Active involvement in research projects related to biological applications of ion beam techniques and generating new applications.
Minimum requirements:
- PhD in Biology/Chemistry/Physics with strong emphasis on cryo-preparation of biological specimens and low temperature electron microscopy
- Experience in operating SEM/TEM and knowledge of EDS technique as well as cryo-ultramicrotomy
- Experience in operating a nuclear microprobe and familiarity with PIXE as an advantage
- Relevant conference presentations and publications as well as international exposure
- Knowledge of statistical methods
- Computer literacy (Word, Excel, Corel, etc.)
We offer a competitive remuneration package, which includes normal company benefits.
Forward your detailed CV, accompanied by a covering letter and supporting documents, to the Human Resource Department; iThemba LABS, P.O. Box 722, Somerset West 7129, or fax (+27-21-8433756), or via e-mail to: vacancies-at-tlabs.ac.za. For some information of the laboratory, visit our website: www.tlabs.ac.za
I suggest that you contact me for any details of this position. E-mail: przybylowicz-at-tlabs.ac.za
You may want to read the following publication on earlier work done by our team in this direction:
Grzegorz Tylko, Jolanta Mesjasz-Przybylowicz and Wojciech J. Przybylowicz. X-ray microanalysis of biological material in the frozen-hydrated state by PIXE. Microscopy Research and Technique (2007) 70: 55-68.
Hi all, I wanna deposit a very thin film of carbon (2 nm) and I found out on Lesker.com that e-beam evaporation and RF sputtering can be used but the films show poor adhesion. As an electrochemist, I am not really sure what to do to enhance the adhesion and I wonder if there is anyone out there who knows how to deposit stable, very thin films of carbon.
Many thanks in advance
Regards
Hany
-- ********************************************************** Hany El-Sayed Graduate student Chemistry department University of Calgary, Alberta Canada 403-220-7306 x: 26322 helsayed-at-ucalgary.ca **********************************************************
==============================Original Headers============================== 5, 27 -- From ramadanhany-at-gmail.com Tue Nov 6 14:40:39 2007 5, 27 -- Received: from wr-out-0506.google.com (wr-out-0506.google.com [64.233.184.238]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA6Kec5N019365 5, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Nov 2007 14:40:39 -0600 5, 27 -- Received: by wr-out-0506.google.com with SMTP id 37so213865wra 5, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 06 Nov 2007 12:40:37 -0800 (PST) 5, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 27 -- d=gmail.com; s=beta; 5, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- bh=m7mSAImEhlinfOhTIRKv4OMdMNfiQB+FCyXiQSOPjjw=; 5, 27 -- b=SCbpwDEz0DYTjD4SW89BYpansNL3lx5ASRD/gvs1RQ6fyhI/hZCdpH1Ae62v1pJjQRHWk4KHwj+rifRiuacZhv5xpSK9ijo89a2fAQ+M3bRxAJoKe+RRZuECsJogT2hg8UayGapIKXdQgi0zpNMrJSUFCOMOVeE4a6phwpj515k= 5, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 27 -- d=gmail.com; s=beta; 5, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- b=I/n7ns+fmswWpHH/o3obxHs/LjpgE/QEGfAKcaIPkoh3zyMBrU7CU7nrvqyYtM2khN19lixV2KvH7agCrhBF8ExkmMVSukzq5DIBsPIWGTjvoI2kEBGMGDmTP7GiuKaXXk3F/mMjhwu8LH8ZrUfZGnMiw+Tzlwo9F5sHXPpdJuo= 5, 27 -- Received: by 10.90.88.13 with SMTP id l13mr4592732agb.1194381637151; 5, 27 -- Tue, 06 Nov 2007 12:40:37 -0800 (PST) 5, 27 -- Received: by 10.90.84.2 with HTTP; Tue, 6 Nov 2007 12:40:37 -0800 (PST) 5, 27 -- Message-ID: {8d8ce5a30711061240n6d21f5f4g33d41fe2fe13f5bd-at-mail.gmail.com} 5, 27 -- Date: Tue, 6 Nov 2007 13:40:37 -0700 5, 27 -- From: "Hany Ramadan" {ramadanhany-at-gmail.com} 5, 27 -- To: Microscopy-at-microscopy.com 5, 27 -- Subject: Carbon thin films deposition 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Type: text/plain; charset=ISO-8859-1 5, 27 -- Content-Transfer-Encoding: 7bit 5, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
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==============================Original Headers============================== 7, 28 -- From didi-at-specs.com Tue Nov 6 14:51:49 2007 7, 28 -- Received: from mail11d.verio-web.com (mail11d.verio-web.com [204.202.242.86]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lA6KpnAY031658 7, 28 -- for {microscopy-at-microscopy.com} ; Tue, 6 Nov 2007 14:51:49 -0600 7, 28 -- Received: from mx77.stngva01.us.mxservers.net (204.202.242.148) 7, 28 -- by mail11d.verio-web.com (RS ver 1.0.95vs) with SMTP id 3-0958862308 7, 28 -- for {microscopy-at-microscopy.com} ; Tue, 6 Nov 2007 15:51:48 -0500 (EST) 7, 28 -- Received: from mmm1125.verio-web.com [161.58.148.83] (EHLO mmm1125.verio-web.com) 7, 28 -- by mx77.stngva01.us.mxservers.net (mxl_mta-1.3.8-10p4) with ESMTP id 572d0374.29942.217.mx77.stngva01.us.mxservers.net; 7, 28 -- Tue, 06 Nov 2007 15:45:41 -0500 (EST) 7, 28 -- Received: (qmail 72698 invoked from network); 6 Nov 2007 20:51:47 -0000 7, 28 -- Received: from unknown (HELO elaine.specs.com) (71.180.247.210) 7, 28 -- by with SMTP; 6 Nov 2007 20:51:47 -0000 7, 28 -- Message-Id: {5.2.1.1.0.20071106154959.024deb38-at-specs.com} 7, 28 -- X-Sender: didi-at-specs.com (Unverified) 7, 28 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 7, 28 -- Date: Tue, 06 Nov 2007 15:51:15 -0500 7, 28 -- To: ramadanhany-at-gmail.com 7, 28 -- From: Dietrich von Diemar {didi-at-specs.com} 7, 28 -- Subject: Re: [Microscopy] Carbon thin films deposition 7, 28 -- Cc: Microscopy-at-microscopy.com 7, 28 -- In-Reply-To: {200711062047.lA6KlXc2031013-at-ns.microscopy.com} 7, 28 -- Mime-Version: 1.0 7, 28 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 28 -- X-Spam: [F=0.0100000000; heur=0.500(-9900); stat=0.010; spamtraq-heur=0.500(2007101727)] 7, 28 -- X-MAIL-FROM: {didi-at-specs.com} 7, 28 -- X-SOURCE-IP: [161.58.148.83] 7, 28 -- X-SF-Loop: 1 ==============================End of - Headers==============================
Job Summary: Chromodynamics Inc. is seeking a Product/Sales Management candidate with a science background and knowledge of the microscope community, strong computer skills and at least one year of hands-on experience using a Confocal or similar advanced light microscope for its new hyperspectral imaging product line.
Location: Orlando, FL or Lakewood, NJ
Responsibilities:
The duties of this position include:
- Customer visits and analysis of customer's imaging needs - Demonstration of products and onsite work with the customer - Sales support of existing customers - Identifying potential new customers via web searches, literature searches, marketing campaigns, trade shows, and customer referrals - Introduction of Chromodynamics products and services to potential customers via in person visits, email and phone - Understanding potential customers needs related to products offered by Chromodynamics Inc. - Organizing workshops and demonstrations for regional sales representatives, distributors and VARs (value-added resellers) - Monitor daily/monthly orders and provide information in regards to account details - Expand sales channels to increase market penetration in designated product areas and markets. - Attain maximum sales and appropriate product mix. - Uncover new sales opportunities through cold calling, networking and other proven marketing strategies - Relay leads to dealer personnel, follow up and monitor outcome - Watch for and identify new markets - Set and achieve established sales goals - Develop thorough knowledge of all frequently encountered applications and their appropriate solutions for which related products are required - Report significant changes or trends in area sales. - Provide feedback on product quality, needs, competition, business trends and unique product applications to management team - Complete required reports - Maintain files and records of customer names, orders and locations - Develop strategic marketing plan and coordinate the sales activities in conjunction with Engineering and Operations to achieve stated goals
Reporting directly to the Vice-President of Marketing and Sales, this key contributor will work with our potential and existing clients, through direct and indirect channels, to meet their life science imaging needs with Chromodynamics Inc. leading solutions and expertise. As the customer's point of contact, they must be able to provide applications assistance, product support and sales of Chromodynamics Inc. products and services to technical buyers. Must have experience responding to tenders, RFPs, RFQs, generating contracts, bid proposals and supporting the negotiation and closing sales with our distributors and representatives. They will follow up on quotations, drive processing of orders and maintain adequate records to document price quotations, decisions, progress and results of activity. They will use available resources such as sales leads, literature, samples and demos, telephones, Internet and email, computers and support staff and services to produce the most effective results and exercise strong administrative, organizational and communication skills.
Minimum Desired Qualifications:
A Bachelors degree in the sciences (i.e. biology, chemistry, physics or equivalent) and a minimum of 4 years experience or a graduate degree or equivalent and a minimum of 2 years of applicable laboratory and sales experience required. Proficient use of personal computers: Windows 95/98, NT, XP, MS Word, Excel, PowerPoint, Internet Explorer, Outlook, etc. Must demonstrate the ability to simplify problems, articulate solutions, lead and delegate, have excellent communication skills and be a highly motivated team oriented self-starter. Travel required up to 25%.
Benefits:
Salary will be based upon background and experience. Chromodynamics, Inc. offers competitive salaries, a comprehensive benefit package including tuition reimbursement, 401(k), and relocation. Chromodynamics Inc. is an Equal Opportunity Employer.
Contact: ChromoDynamics, Inc. 1195 Airport Road, #1 Lakewood, New Jersey 08701 Ph: 732.730.1877 Fax: 732.730.3547 Email: careers-at-olinet.com Web: http://www.chromodynamics.net/
==============================Original Headers============================== 13, 32 -- From afong-at-olinet.com Tue Nov 6 15:16:43 2007 13, 32 -- Received: from renoir.hmdnsgroup.com (renoir.hmdnsgroup.com [63.247.141.9]) 13, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA6LGhts011859 13, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Nov 2007 15:16:43 -0600 13, 32 -- Received: from [216.189.208.196] (port=1560 helo=VPSales) 13, 32 -- by renoir.hmdnsgroup.com with esmtpa (Exim 4.68) 13, 32 -- (envelope-from {afong-at-olinet.com} ) 13, 32 -- id 1IpVms-0005RK-EU 13, 32 -- for Microscopy-at-microscopy.com; Tue, 06 Nov 2007 16:16:43 -0500 13, 32 -- Reply-To: {afong-at-olinet.com} 13, 32 -- From: "Alex Fong" {afong-at-olinet.com} 13, 32 -- To: {Microscopy-at-microscopy.com} 13, 32 -- Date: Tue, 6 Nov 2007 16:16:20 -0500 13, 32 -- Organization: Optronic Laboratories 13, 32 -- Message-ID: {01c701c820ba$53d5de30$4214a8c0-at-VPSales} 13, 32 -- MIME-Version: 1.0 13, 32 -- Content-Type: text/plain; 13, 32 -- charset="us-ascii" 13, 32 -- Content-Transfer-Encoding: 7bit 13, 32 -- X-Mailer: Microsoft Office Outlook 11 13, 32 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 13, 32 -- Thread-Index: AcggukQPY3S3Pvu5SCibNHPGZTsi5A== 13, 32 -- X-HMDNSGroup-MailScanner-Information: Please contact the ISP for more information 13, 32 -- X-HMDNSGroup-MailScanner: Found to be clean 13, 32 -- X-HMDNSGroup-MailScanner-SpamCheck: 13, 32 -- X-HMDNSGroup-MailScanner-From: afong-at-olinet.com 13, 32 -- Subject: Job Posting: Product Marketing/Sales Manager 13, 32 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 13, 32 -- X-AntiAbuse: Primary Hostname - renoir.hmdnsgroup.com 13, 32 -- X-AntiAbuse: Original Domain - microscopy.com 13, 32 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 13, 32 -- X-AntiAbuse: Sender Address Domain - olinet.com ==============================End of - Headers==============================
Job Responsibilities: -Conduct activities to support screening analyses. -Conduct activities to support development and validation of new histological markers. -Be responsible for data analysis and called upon to prepare experimental reports. -Interact with other reseach departments in R&D.
Education: BSc in Biology or any other related field
Experience Required: -At least 3 years experience, preferably in pharmaceutical or biotechnological industry. -Knowledge of histological techniques, especially in preparation of frozen sections on a cryostat or a freezing microtome. -Histological and immunohistochemical staining. -Practical experience in image analysis (bright field and fluorescence) is a major asset.
Other Requirements: -Candidate is expected to be meticulous, accurate, and autonomous in execution of tasks. -Team spirit, initiative, accurate judgement, and keen organizational skills. -Knowledge of the Microsoft Office suite. -Good commnicating skills in both English and French are essential.
Submit resume at the website: www.neurochem.com under the career tab.
Marie-Claude Belanger Montreal _________________________________________________________________ Envoie un sourire, fais rire, amuse-toi! Employez-le maintenant! http://www.emoticonesgratuites.ca/?icid=EMFRCA120
==============================Original Headers============================== 11, 18 -- From mcbelanger6-at-hotmail.com Tue Nov 6 16:41:00 2007 11, 18 -- Received: from bay0-omc3-s7.bay0.hotmail.com (bay0-omc3-s7.bay0.hotmail.com [65.54.246.207]) 11, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA6MexTw025806 11, 18 -- for {microscopy-at-microscopy.com} ; Tue, 6 Nov 2007 16:41:00 -0600 11, 18 -- Received: from BAY122-W21 ([207.46.10.184]) by bay0-omc3-s7.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.3959); 11, 18 -- Tue, 6 Nov 2007 14:40:59 -0800 11, 18 -- Message-ID: {BAY122-W218D0A420074298D22F0AFE3890-at-phx.gbl} 11, 18 -- X-Originating-IP: [66.46.83.20] 11, 18 -- From: =?iso-8859-1?Q?Marie-Claude_B=E9langer?= {mcbelanger6-at-hotmail.com} 11, 18 -- To: {microscopy-at-microscopy.com} 11, 18 -- Subject: LM-Position in Histology 11, 18 -- Date: Tue, 6 Nov 2007 22:40:58 +0000 11, 18 -- Importance: Normal 11, 18 -- Content-Type: text/plain; charset="iso-8859-1" 11, 18 -- MIME-Version: 1.0 11, 18 -- X-OriginalArrivalTime: 06 Nov 2007 22:40:59.0697 (UTC) FILETIME=[1A4DAA10:01C820C6] 11, 18 -- Content-Transfer-Encoding: 8bit 11, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lA6MexTw025806 ==============================End of - Headers==============================
May I have your guys options about the test method of ice contamination. I have been suggested to use following way to test whether there is an ice contamination or not during the cryoTEM session:
Cooling down a plain carbon film grid in LN2. Cold transfer it into microscope and focus the beam at lower magnification (5000X). It shall be able to see a burning area on the carbon film and then brings the temperature up. This area would disappear during the temperature up so this is suggestion that there is an ice contamination.
Your options are highly appreciated.
Peiyi
==============================Original Headers============================== 5, 27 -- From P.Wang-at-sheffield.ac.uk Wed Nov 7 06:24:03 2007 5, 27 -- Received: from marmot.shef.ac.uk (marmot.shef.ac.uk [143.167.1.4]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA7CO27n001818 5, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Nov 2007 06:24:03 -0600 5, 27 -- Received: from drake.shef.ac.uk ([143.167.2.64]) 5, 27 -- by marmot.shef.ac.uk with esmtp (Exim 4.52) 5, 27 -- id 1Ipjwm-0000TC-Fl; Wed, 07 Nov 2007 12:23:52 +0000 5, 27 -- Received: from http by drake.shef.ac.uk with local (Exim 4.42) 5, 27 -- id 1Ipjwm-0002oi-Ci; Wed, 07 Nov 2007 12:23:52 +0000 5, 27 -- Received: from drake.shef.ac.uk (drake.shef.ac.uk [143.167.2.64]) 5, 27 -- by webmail.shef.ac.uk (IMP) with HTTP 5, 27 -- for {mb1pyw-at-localhost} ; Wed, 7 Nov 2007 12:23:51 +0000 5, 27 -- Message-ID: {1194438231.4731ae57b0d89-at-webmail.shef.ac.uk} 5, 27 -- Date: Wed, 7 Nov 2007 12:23:51 +0000 5, 27 -- From: P Wang {P.Wang-at-sheffield.ac.uk} 5, 27 -- To: Bill Tivol {tivol-at-caltech.edu} 5, 27 -- Cc: 3dem-at-ucsd.edu, Microscopy-at-microscopy.com 5, 27 -- Subject: Ice contamination 5, 27 -- References: {7545957e8ff43b5c5639d88be76e0421-at-sfu.ca} {5DF105B9-3036-43E0-A648-88ABEEF4BDF3-at-caltech.edu} 5, 27 -- In-Reply-To: {5DF105B9-3036-43E0-A648-88ABEEF4BDF3-at-caltech.edu} 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Type: text/plain; charset=ISO-8859-1 5, 27 -- Content-Transfer-Encoding: 8bit 5, 27 -- User-Agent: Internet Messaging Program (IMP) 3.2.2 5, 27 -- Organization: University of Sheffield 5, 27 -- X-Originating-IP: 143.167.2.64 5, 27 -- X-S0phie-Scan: no ==============================End of - Headers==============================
Many thanks for the reply. I might not state very clearly for the proposed of this test. The suggestion of this method is to be used to find out whether there is ice contamination during the cold transfer or not. On other word is that can we know where the ice contamination came from if there is any when we just open the gun valves by this method without knowing previous condition of the carbon film.
} Hi Peiyi, } } Better yet, if you have a gatan holder. Put the grid in the holder at } room temperature. Put it in the scope and then cool the holder down while } it is in the scope. When it is cold, let it sit in the scope for a while. } When its been there for a while, go to a higher mag and put the beam on } the carbon with the beam expanded to just the size of the viewing screen. } Let it sit for a while, then go down in mag. If there is contamination, } you will see a round foot print of the beam used at the higher mag on the } lower mag image you see on the screen. The suggestion you have below } would also work but if contamination is slow or very little, the } contamination would be gone fast after the beam exposes the area before } you see burning. } } angel } } On Wed, 7 Nov 2007 P.Wang-at-sheffield.ac.uk wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Dear all, } } } } May I have your guys options about the test method of ice contamination. I } have } } been suggested to use following way to test whether there is an ice } } contamination or not during the cryoTEM session: } } } } Cooling down a plain carbon film grid in LN2. Cold transfer it into } microscope } } and focus the beam at lower magnification (5000X). It shall be able to see } a } } burning area on the carbon film and then brings the temperature up. This } area } } would disappear during the temperature up so this is suggestion that there } is } } an ice contamination. } } } } Your options are highly appreciated. } } } } Peiyi } } }
==============================Original Headers============================== 6, 26 -- From P.Wang-at-sheffield.ac.uk Wed Nov 7 10:34:39 2007 6, 26 -- Received: from marmot.shef.ac.uk (marmot.shef.ac.uk [143.167.1.4]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA7GYdwO029067 6, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Nov 2007 10:34:39 -0600 6, 26 -- Received: from drake.shef.ac.uk ([143.167.2.64]) 6, 26 -- by marmot.shef.ac.uk with esmtp (Exim 4.52) 6, 26 -- id 1IpnrQ-0001o4-3z; Wed, 07 Nov 2007 16:34:36 +0000 6, 26 -- Received: from http by drake.shef.ac.uk with local (Exim 4.42) 6, 26 -- id 1IpnrP-0007b0-R6; Wed, 07 Nov 2007 16:34:35 +0000 6, 26 -- Received: from drake.shef.ac.uk (drake.shef.ac.uk [143.167.2.64]) 6, 26 -- by webmail.shef.ac.uk (IMP) with HTTP 6, 26 -- for {mb1pyw-at-localhost} ; Wed, 7 Nov 2007 16:34:35 +0000 6, 26 -- Message-ID: {1194453275.4731e91baf360-at-webmail.shef.ac.uk} 6, 26 -- Date: Wed, 7 Nov 2007 16:34:35 +0000 6, 26 -- From: P Wang {P.Wang-at-sheffield.ac.uk} 6, 26 -- To: David Stokes {stokes-at-saturn.med.nyu.edu} , 6, 26 -- Angel Paredes {Angel.Paredes-at-uth.tmc.edu} 6, 26 -- Cc: 3dem-at-ucsd.edu, Microscopy-at-microscopy.com 6, 26 -- Subject: Re: [Microscopy] Ice contamination 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1 6, 26 -- Content-Transfer-Encoding: 8bit 6, 26 -- User-Agent: Internet Messaging Program (IMP) 3.2.2 6, 26 -- Organization: University of Sheffield 6, 26 -- X-Originating-IP: 143.167.2.64 6, 26 -- X-S0phie-Scan: no ==============================End of - Headers==============================
Peiyi, The sort of ice that comes from a poor transfer is not subtle. It is large multi-micron sized boulders (high contrast) of crystilline ice. It is obvious if this sort of contamination is present. The sort of ice that slowly builds up because of a poor vacuum is what David and Angel are testing for. Hope this helps. Regards, Bob
P Wang wrote: } Dear David and Angel, } } Many thanks for the reply. I might not state very clearly for the proposed of } this test. The suggestion of this method is to be used to find out whether } there is ice contamination during the cold transfer or not. On other word is } that can we know where the ice contamination came from if there is any when we } just open the gun valves by this method without knowing previous condition of } the carbon film. } } Cheers. } } Peiyi } } } Quoting Angel Paredes {Angel.Paredes-at-uth.tmc.edu} : } } } } Hi Peiyi, } } } } Better yet, if you have a gatan holder. Put the grid in the holder at } } room temperature. Put it in the scope and then cool the holder down while } } it is in the scope. When it is cold, let it sit in the scope for a while. } } When its been there for a while, go to a higher mag and put the beam on } } the carbon with the beam expanded to just the size of the viewing screen. } } Let it sit for a while, then go down in mag. If there is contamination, } } you will see a round foot print of the beam used at the higher mag on the } } lower mag image you see on the screen. The suggestion you have below } } would also work but if contamination is slow or very little, the } } contamination would be gone fast after the beam exposes the area before } } you see burning. } } } } angel } } } } On Wed, 7 Nov 2007 P.Wang-at-sheffield.ac.uk wrote: } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- } } } } } http://www.microscopy.com/MicroscopyListserver } } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } } } ---------------------------------------------------------------------------- } } } } } Dear all, } } } } } } May I have your guys options about the test method of ice contamination. I } } } } } have } } } } } been suggested to use following way to test whether there is an ice } } } contamination or not during the cryoTEM session: } } } } } } Cooling down a plain carbon film grid in LN2. Cold transfer it into } } } } } microscope } } } } } and focus the beam at lower magnification (5000X). It shall be able to see } } } } } a } } } } } burning area on the carbon film and then brings the temperature up. This } } } } } area } } } } } would disappear during the temperature up so this is suggestion that there } } } } } is } } } } } an ice contamination. } } } } } } Your options are highly appreciated. } } } } } } Peiyi } } } } } } } _______________________________________________ } 3dem mailing list } 3dem-at-ncmir.ucsd.edu } https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem } }
-- ********************************
-Robert Grassucci- Howard Hughes Medical Institute Wadsworth Center Empire State Plaza Albany, NY 12201-0509 bobg-at-wadsworth.org Phone: (518)474-5821 Fax: (518)486-2191
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==============================Original Headers============================== 11, 28 -- From bob.grassucci-at-wadsworth.org Wed Nov 7 11:45:25 2007 11, 28 -- Received: from mailserv.wadsworth.org (mailserv.wadsworth.org [199.184.30.43]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA7HjPwU010817 11, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Nov 2007 11:45:25 -0600 11, 28 -- Received: from [127.0.0.1] (newton12-119 [172.16.12.119]) 11, 28 -- by mailserv.wadsworth.org (8.13.8+Sun/8.13.8) with ESMTP id lA7HjLNA020855; 11, 28 -- Wed, 7 Nov 2007 12:45:21 -0500 (EST) 11, 28 -- Message-ID: {4731F9B0.5060006-at-wadsworth.org} 11, 28 -- Date: Wed, 07 Nov 2007 12:45:20 -0500 11, 28 -- From: Bob Grassucci {bob.grassucci-at-wadsworth.org} 11, 28 -- Organization: HHMI 11, 28 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 11, 28 -- MIME-Version: 1.0 11, 28 -- To: P Wang {P.Wang-at-sheffield.ac.uk} 11, 28 -- CC: David Stokes {stokes-at-saturn.med.nyu.edu} , 11, 28 -- Angel Paredes {Angel.Paredes-at-uth.tmc.edu} , Microscopy-at-microscopy.com, 11, 28 -- 3dem-at-ucsd.edu 11, 28 -- Subject: Re: [3dem] Re: [Microscopy] Ice contamination 11, 28 -- References: {1194453275.4731e91baf360-at-webmail.shef.ac.uk} 11, 28 -- In-Reply-To: {1194453275.4731e91baf360-at-webmail.shef.ac.uk} 11, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit 11, 28 -- X-Wadsworth-MailScanner-Information: Please contact CSS for more information 11, 28 -- X-Wadsworth-MailScanner: Found to be clean 11, 28 -- X-Wadsworth-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, 11, 28 -- score=-100.002, required 5, autolearn=not spam, SPF_HELO_PASS -0.00, 11, 28 -- SPF_PASS -0.00, USER_IN_SPF_WHITELIST -100.00) 11, 28 -- X-MailScanner-From: bob.grassucci-at-wadsworth.org ==============================End of - Headers==============================
On Nov 6, 2007, at 3:31 AM, jacques.faerber-at-ipcms.u-strasbg.fr wrote:
} As we are in workings for our new TEM, we have to watch closely to } avoid } that the relative clean environnement we have found wont be crabed } by a } bad electrical wiring or something else like that. } } So my question is on UPS. What about EM interferencies generated by an } 10 kVA UPS. Is that a concern for HR TEM/STEM, HR-SEM, etc, or are the } level too low, and the frequency generated too high to be a source of } problem ? The spec I've read gives only caracteristics in a 150 kHz } - 30 } MHz, but nothing in the ~0 - 20 kHz. } } Of coarse distance is the easiest way to limit the effects of possible } interferencies radiated by the UPS itself, but we don't want to be on } the safe side, and, for exemple, put the UPS too far away... and catch } again interferencies from the environnement by the needed long cable. } } What are the feedbacks form UPS users. Until now, we don't have UPS in } use, on the EM equipements.
Dear Jacques, We have a UPS on our FEI Polara TEM. It and the electronics are in an equipment room adjacent to the scope room, and there is some shielding in the wall separating the two rooms. The input goes through both autotransformers and the UPS, then to the electronics and the HT supply and scope. We were concerned that both the autotransformers and the UPS have large coils that could be magnetic field sources, but we have not had any problem with fields at the scope. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 5, 22 -- From tivol-at-caltech.edu Wed Nov 7 12:34:25 2007 5, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA7IYPBO024026 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 7 Nov 2007 12:34:25 -0600 5, 22 -- Received: from water-dog.its.caltech.edu (water-dog [192.168.1.26]) 5, 22 -- by wood-ox-postvirus (Postfix) with ESMTP id 24B8413DBB 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 7 Nov 2007 10:34:23 -0800 (PST) 5, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 5, 22 -- by wood-ox.its.caltech.edu (Postfix) with ESMTP id 1CBBE13B88 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 7 Nov 2007 10:34:19 -0800 (PST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v752.3) 5, 22 -- In-Reply-To: {200711061131.lA6BVjdj032408-at-ns.microscopy.com} 5, 22 -- References: {200711061131.lA6BVjdj032408-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {38FC5775-511C-4FB8-ACA8-9D0C0E551CA1-at-caltech.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 22 -- Subject: Re: [Microscopy] UPS, EM interferncies and TEM quest. 5, 22 -- Date: Wed, 7 Nov 2007 10:34:36 -0800 5, 22 -- To: microscopy-at-msa.microscopy.com 5, 22 -- X-Mailer: Apple Mail (2.752.3) 5, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.4.5 ==============================End of - Headers==============================
I have a series of images of particles arranged in a hexagonal pattern on a substrate. The particles can be either: - arranged in a perfectly hexagonal pattern or; - very close to a hexagonal pattern, but not perfect; - quite disordered but somewhat hexagonal pattern; - completely disordered;
Can anyone point me to a technique (description or literature) - probably FFT - to properly characterize how well the particles fit the intended order (hexagonal)? It is obvious from the images, but would be nice to put a number on it.
Thank you, Daniel
Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC 11421 Saskatchewan Dr. Edmonton, AB. T6G 2M9
Phone: (780) 641-1663 Fax: (780) 641-1601
==============================Original Headers============================== 10, 23 -- From Daniel.Salamon-at-nrc-cnrc.gc.ca Wed Nov 7 15:38:04 2007 10, 23 -- Received: from nrccenexf2.nrc.ca (nrccenexf2.nrc.ca [132.246.15.83]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA7Lc3IR008908 10, 23 -- for {microscopy-at-microscopy.com} ; Wed, 7 Nov 2007 15:38:03 -0600 10, 23 -- Received: from nrccenexb2.nrc.ca ([132.246.15.98]) by nrccenexf2.nrc.ca with Microsoft SMTPSVC(6.0.3790.3959); 10, 23 -- Wed, 7 Nov 2007 16:38:03 -0500 10, 23 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 10, 23 -- Content-class: urn:content-classes:message 10, 23 -- MIME-Version: 1.0 10, 23 -- Content-Type: text/plain; 10, 23 -- charset="US-ASCII" 10, 23 -- Subject: particle order analysis 10, 23 -- Date: Wed, 7 Nov 2007 16:38:02 -0500 10, 23 -- Message-ID: {923687253229634E96E808B8D3124C83015BA526-at-nrccenexb2.nrc.ca} 10, 23 -- X-MS-Has-Attach: 10, 23 -- X-MS-TNEF-Correlator: 10, 23 -- Thread-Topic: particle order analysis 10, 23 -- Thread-Index: Acghhnk17hqxQ8izQBGmXNYuT0gwmw== 10, 23 -- From: "Salamon, Daniel" {Daniel.Salamon-at-nrc-cnrc.gc.ca} 10, 23 -- To: {microscopy-at-microscopy.com} 10, 23 -- X-OriginalArrivalTime: 07 Nov 2007 21:38:03.0354 (UTC) FILETIME=[79D733A0:01C82186] 10, 23 -- Content-Transfer-Encoding: 8bit 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lA7Lc3IR008908 ==============================End of - Headers==============================
Thanks to all who have replied, and given references and advice.
For those of you who are interested in this thread, the methods suggested were staining, freeze fracture, and cryo TEM (it wasn't such a bad idea after all).
I can't tell which posts were sent list-wide, so if anyone wants a more detailed summary, let me know.
Thanks again.
Sincerely, Jessica Cervantes Bend Research Inc Bend, OR 97701 www.bendres.com
==============================Original Headers============================== 9, 14 -- From cervantes-at-bendres.com Wed Nov 7 16:07:18 2007 9, 14 -- Received: from mail.bendres.com (mail.bendres.com [216.228.161.112] (may be forged)) 9, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA7M7IAA021813 9, 14 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Nov 2007 16:07:18 -0600 9, 14 -- MIME-Version: 1.0 9, 14 -- Content-Type: text/plain; 9, 14 -- charset="us-ascii" 9, 14 -- Subject: TEM Help with Imaging Micelles 9, 14 -- Date: Wed, 7 Nov 2007 14:07:17 -0800 9, 14 -- Message-ID: {8943D65F9AD70E4488AD6DE09F15088768C84C-at-BRIEX04A} 9, 14 -- From: "Cervantes, Jessica" {cervantes-at-bendres.com} 9, 14 -- To: {Microscopy-at-microscopy.com} 9, 14 -- Content-Transfer-Encoding: 8bit 9, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lA7M7IAA021813 ==============================End of - Headers==============================
Sorry, I forgot to mention we are trying to analyze particles that are sitting apart (not close-packed) in one layer on a surface with good contrast - equivalent to an array of separate white dots on a dark background.
Thanks, Daniel
-----Original Message----- X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca [mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca] Sent: Wednesday, November 07, 2007 2:41 PM To: Salamon, Daniel
Hello everyone,
Thank you in advance for your help.
I have a series of images of particles arranged in a hexagonal pattern on a substrate. The particles can be either: - arranged in a perfectly hexagonal pattern or; - very close to a hexagonal pattern, but not perfect; - quite disordered but somewhat hexagonal pattern; - completely disordered;
Can anyone point me to a technique (description or literature) - probably FFT - to properly characterize how well the particles fit the intended order (hexagonal)? It is obvious from the images, but would be nice to put a number on it.
Thank you, Daniel
Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC 11421 Saskatchewan Dr. Edmonton, AB. T6G 2M9
Phone: (780) 641-1663 Fax: (780) 641-1601 =====
==============================Original Headers============================== 16, 25 -- From Daniel.Salamon-at-nrc-cnrc.gc.ca Wed Nov 7 17:03:21 2007 16, 25 -- Received: from nrccenexf1.nrc.ca (nrccenexf1.nrc.ca [132.246.15.80]) 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA7N3KG8002496 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 7 Nov 2007 17:03:21 -0600 16, 25 -- Received: from nrccenexb2.nrc.ca ([132.246.15.98]) by nrccenexf1.nrc.ca with Microsoft SMTPSVC(6.0.3790.3959); 16, 25 -- Wed, 7 Nov 2007 18:03:20 -0500 16, 25 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 16, 25 -- Content-class: urn:content-classes:message 16, 25 -- MIME-Version: 1.0 16, 25 -- Content-Type: text/plain; 16, 25 -- charset="US-ASCII" 16, 25 -- Subject: clarification: particle order analysis 16, 25 -- Date: Wed, 7 Nov 2007 18:03:19 -0500 16, 25 -- Message-ID: {923687253229634E96E808B8D3124C83015BA578-at-nrccenexb2.nrc.ca} 16, 25 -- In-Reply-To: {200711072141.lA7LfSxu012876-at-ns.microscopy.com} 16, 25 -- X-MS-Has-Attach: 16, 25 -- X-MS-TNEF-Correlator: 16, 25 -- Thread-Topic: clarification: particle order analysis 16, 25 -- Thread-Index: AcghhvVs/AvtM56fR5K1M18KkUZu4QACw5pg 16, 25 -- References: {200711072141.lA7LfSxu012876-at-ns.microscopy.com} 16, 25 -- From: "Salamon, Daniel" {Daniel.Salamon-at-nrc-cnrc.gc.ca} 16, 25 -- To: {microscopy-at-microscopy.com} 16, 25 -- X-OriginalArrivalTime: 07 Nov 2007 23:03:20.0671 (UTC) FILETIME=[63FFCEF0:01C82192] 16, 25 -- Content-Transfer-Encoding: 8bit 16, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lA7N3KG8002496 ==============================End of - Headers==============================
Tutorials recorded at M&M 2007 are now available. These are listed below. Please refer to the MSA website for details of ordering
299 High pressure freezing for electron microscopy of biological specimens. AND Freeze substitution method: tutorial and roundtable Discussion Instructors: Paul Walther, Daniel Studer, Kent McDonald 2007 $25.00
300 A novel sample freezing method Instructor: Jan Leunissen 2007 $25.00
301 Electron tomography for materials science Instructor: Paul Midgley 2007 $25.00
302 LACSBI: incoherent imaging for quantitative TEM Instructor: Ian Anderson 2007 $25.00
303 Atomic force microscopy (AFM) and related microscopy Techniques and applications Instructor: Phil Russell 2007 $25.00
304 Creating a successful scientific presentation (Professional Development tutorial) Instructor: Bev Maleef 2007 $25.00
305 Playing the grant game to get the toys (instruments) we want (Professional Development tutorial) Instructor: Bob Price 2007 $25.00
306 X-ray microCT Instructor: Stuart Stock 2007 $25.00
-- Greg Erdos University of Florida, Retired Micanopy, Florida
==============================Original Headers============================== 13, 23 -- From gwe-at-ufl.edu Thu Nov 8 08:59:41 2007 13, 23 -- Received: from smtp.ufl.edu (smtp02.osg.ufl.edu [128.227.74.165]) 13, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA8ExeOU008219 13, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 8 Nov 2007 08:59:40 -0600 13, 23 -- Received: from [10.228.0.108] (ssrb-vpn1-0-108.vpn.ufl.edu [10.228.0.108]) 13, 23 -- (authenticated bits=0) 13, 23 -- by smtp.ufl.edu (8.13.7/8.13.7/2.5.9) with ESMTP id lA8Excx43555414 13, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 8 Nov 2007 09:59:39 -0500 13, 23 -- Message-ID: {4733245C.6030403-at-ufl.edu} 13, 23 -- Date: Thu, 08 Nov 2007 09:59:40 -0500 13, 23 -- From: Greg Erdos {gwe-at-ufl.edu} 13, 23 -- Reply-To: gwe-at-ufl.edu 13, 23 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 13, 23 -- MIME-Version: 1.0 13, 23 -- To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com} 13, 23 -- Subject: New Tutorial DVDs Now Available 13, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 23 -- Content-Transfer-Encoding: 7bit 13, 23 -- X-Greylist: Sender succeeded SMTP AUTH authentication, not delayed by milter-greylist-3.0rc3 (smtp.ufl.edu [128.227.74.56]); Thu, 08 Nov 2007 09:59:39 -0500 (EST) 13, 23 -- X-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 13, 23 -- X-UFL-Spam-Status: hits=-1.44, required=5, tests=ALL_TRUSTED 13, 23 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 13, 23 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
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Email: smurray-at-picr.man.ac.uk Name: Steve Murray
Organization: Paterson Institute, University of Manchester, UK
Title-Subject: [Filtered] Equipment Available
Question: Following the closure of the SEM unit and the purchase of new equipment, I have several items of equipment available for which I am open to offers. Bal-Tec CPD 030, less than five years old. Leica AFS with UV head and all the trimmings. Edwards Plate degassers *2 (One is about three years old and sits on the RV3 rotary pump, the other is much older and sits on an EM2). Edwards pump station comprising the EXT70H turbo backed by an XDD1 diaphragm with EXT120 controller and with an Active gauge control unit, no gauges. This unit has never been used, it was purchased as part of a package with another equipment item, but for various reasons was never put into service. New the whole set would cost around £5k, happy to take £2k. I also have available a spare set of film plate carriers for a JEOL 1220 TEM and the film boxes. I think there are about 80 carriers, but I would have to check on that. Offers invited Finally, I have a SiS MegaviewII CCD side mount from a JEOL 1220 with the relevant grabber board cabling and pneumatic unit (no PC)in use until very recently. I have just replaced it with a Gatan Orius.
The Dept. of Materials at Oxford University has an open position for a post-doctoral researcher in transmission electron microscopy of magnetic materials.
Grade 7: Salary in the range £26,666 - £28,289 p.a.
We have a vacancy from April 2008 (or as soon as possible thereafter) for up to two years, working for Dr Amit Kohn on antiferromagnetic thin films used for spin electronics.
Spin electronics is a novel field aimed at adding the spin degree of freedom to conventional charge-based electronic devices. A key component in these devices is antiferromagnetic metallic thin films. However, the magnetic properties of these films are still not well understood because of the inability to resolve their micromagnetic structure.
This project, which is funded by the EPSRC, is aimed at understanding the correlation between microstructure and micromagnetic properties in these technologically important antiferromagnetic metallic thin films. The research will be a collaboration with the theoretical group of Professor Thomas Schrefl at Sheffield University. We will develop new simulation codes and transmission electron microscopy imaging methodologies of antiferromagnetic domains in metallic films at the nanometre scale.
You will be responsible for the experimental work in this project by studying the microstructure and micromagnetic properties of antiferromagnetic films using advanced transmission electron microscopy. You will work in the Magnetic Materials Group, which is part of the Electron Microscopy and Microanalysis Group. This materials research will be an essential part of the project effort towards the development of new models and micromagnetic simulation codes on antiferromagnetic metallic thin films. The characterisation will be undertaken predominately using in-situ Lorentz TEM and a range of transmission electron microscopy techniques including high-resolution TEM and analytical techniques such as energy-filtered imaging (EELS) and EDX.
You should have a doctorate in materials science, physics or a related discipline on magnetic materials or devices. Knowledge of magnetism, magnetic materials/devices is required. Prior research on antiferromagnetic material, in particular exchange-bias, is highly advantageous. Experience in transmission electron microscopy, preferably at an advanced level, is required as is experience in TEM sample preparation (mechanical polishing and FIB).
This position will require the PDRA to become proficient with Lorentz TEM. Evidence of a strong publication record commensurate with your stage of career is expected. Proven ability to identify research objectives and meet agreed deadlines, self-motivation and flexibility are essential. Excellent written and communication skills in English and the ability to work effectively as part of a team are required, as is willingness to travel for short periods to work with collaborators at Sheffield University.
Further particulars, including instructions on applying for this post, are available from the web-site: http://www.materials.ox.ac.uk/ or from Mrs K Fewings, Department of Materials, University of Oxford, Parks Road, Oxford OX1 3PH (email: posts-at-materials.ox.ac.uk ), or telephone 01865 273680 (post reference DJ07/026).
The closing date for applications is 4 January 2008 with interviews currently planned for the week starting 28 January 2008.
==============================Original Headers============================== 13, 27 -- From amit.kohn-at-materials.ox.ac.uk Thu Nov 8 10:45:05 2007 13, 27 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk [163.1.2.169]) 13, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA8Gj4Q9014693 13, 27 -- for {Microscopy-at-Microscopy.Com} ; Thu, 8 Nov 2007 10:45:04 -0600 13, 27 -- Received: from smtp0.mail.ox.ac.uk ([129.67.1.205]) 13, 27 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.68) 13, 27 -- (envelope-from {amit.kohn-at-materials.ox.ac.uk} ) 13, 27 -- id 1IqAV5-0006AK-Vf 13, 27 -- for Microscopy-at-Microscopy.Com; Thu, 08 Nov 2007 16:45:03 +0000 13, 27 -- Received: from oums-kohn.materials.ox.ac.uk ([129.67.84.55] helo=OUMSKOHN) 13, 27 -- by smtp0.mail.ox.ac.uk with esmtp (Exim 4.68) 13, 27 -- (envelope-from {amit.kohn-at-materials.ox.ac.uk} ) 13, 27 -- id 1IqAV5-0005Fd-1V 13, 27 -- for Microscopy-at-Microscopy.Com; Thu, 08 Nov 2007 16:45:03 +0000 13, 27 -- From: "Amit Kohn" {amit.kohn-at-materials.ox.ac.uk} 13, 27 -- To: {Microscopy-at-Microscopy.Com} 13, 27 -- Subject: TEM position (DJ07_026) - Oxford University: Magnetic Materials 13, 27 -- Date: Thu, 8 Nov 2007 16:45:14 -0000 13, 27 -- Message-ID: {000901c82226$bc42b890$37544381-at-OUMSKOHN} 13, 27 -- MIME-Version: 1.0 13, 27 -- Content-Type: text/plain; 13, 27 -- charset="iso-8859-1" 13, 27 -- X-Mailer: Microsoft Office Outlook 11 13, 27 -- Thread-Index: AcghPiBOaHdQetpnR/WU5rGQDTWkGgA6IMxA 13, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 13, 27 -- Content-Transfer-Encoding: 8bit 13, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lA8Gj4Q9014693 ==============================End of - Headers==============================
I have a problem with a Philips 201 TEM and wonder if anyone has a suggestion.
The high vacuum valve, driven by a magnetic coil, is not holding in place. When the situation is right for the valve to open you can here it going "click, click, click" but it won't stay in place.
It has on occasion stuck closed before but an encouraging tap with a screwdriver handle on the housing always took care of that!
Any suggestions would be appreciated.
Many thanks,
Ted Dunn The EMscope Company Ltd. Thailand
66 81-739-4259
__________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com
==============================Original Headers============================== 10, 19 -- From drteddunne-at-yahoo.com Thu Nov 8 12:58:28 2007 10, 19 -- Received: from web33414.mail.mud.yahoo.com (web33414.mail.mud.yahoo.com [68.142.206.146]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lA8IwRM2005360 10, 19 -- for {Microscopy-at-Microscopy.com} ; Thu, 8 Nov 2007 12:58:27 -0600 10, 19 -- Received: (qmail 3316 invoked by uid 60001); 8 Nov 2007 18:58:27 -0000 10, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 10, 19 -- s=s1024; d=yahoo.com; 10, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 10, 19 -- b=xsHD4YBNV/w3ChT8Jq/rNTSnA2PRzK5nyAG6tmFdZtxDy1vKyfnv7utUFXaM/3iSc4Yin1bPKtpXIzpG99qpfCDnRTdckV/pLtYPYg4wM/Kg070TBi0cbCG5QqIuTZPAenlMr/DHNAAoGHa5SHPqAFhiXinvc3qW7KOzPDNtibc=; 10, 19 -- X-YMail-OSG: fNHDcuQVM1mzq55dDQGxEB232j0lo2GhyzbzXJeYdtPozr0C7HmrSJNy05Ca.iDFOrlzv6VsLn.F40F867sU3GCMHuOCmFyWblne.axEUpRLjni.gD50jU4arCJRuA-- 10, 19 -- Received: from [125.24.241.61] by web33414.mail.mud.yahoo.com via HTTP; Thu, 08 Nov 2007 10:58:27 PST 10, 19 -- X-Mailer: YahooMailRC/818.27 YahooMailWebService/0.7.152 10, 19 -- Date: Thu, 8 Nov 2007 10:58:27 -0800 (PST) 10, 19 -- From: ted dunn {drteddunne-at-yahoo.com} 10, 19 -- Subject: Philips 201 10, 19 -- To: Microscopy-at-Microscopy.com 10, 19 -- MIME-Version: 1.0 10, 19 -- Content-Type: text/plain; charset=us-ascii 10, 19 -- Message-ID: {445564.2708.qm-at-web33414.mail.mud.yahoo.com} ==============================End of - Headers==============================
As a cautionary tale I relate an incident that happened in our lab recently.
Someone placed 0.25g of osmium tetroxide crystals into a dry, clean 50ml polypropylene Falcon tube and it immediately burst into flames.
The inside of the tube turned black and the crystals disappeared. Luckily she was working in the chemical hood so no harm was done.
As I have been working with osmium tetroxide for many years with no problem, it was a surprise to me that this chemical could burst into flames so easily. Can anyone offer any insight on why this might happen?
One reason why this may not have happened to me is that I always follow the good advice of adding solid to liquid.
Regards,
Paul Webster.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
==============================Original Headers============================== 12, 18 -- From PWebster-at-hei.org Thu Nov 8 13:34:59 2007 12, 18 -- Received: from hi0sml1.hei.org (House-Ear-Institute-1114861.cust-rtr.pacbell.net [71.136.31.242]) 12, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA8JYwEJ019039 12, 18 -- for {Microscopy-at-Microscopy.com} ; Thu, 8 Nov 2007 13:34:59 -0600 12, 18 -- Received: from 10.10.42.170 ([10.10.42.170]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 12, 18 -- Thu, 8 Nov 2007 19:34:58 +0000 12, 18 -- User-Agent: Microsoft-Entourage/11.3.6.070618 12, 18 -- Date: Thu, 08 Nov 2007 11:34:58 -0800 12, 18 -- Subject: Osmium ignition 12, 18 -- From: "Webster, Paul" {PWebster-at-hei.org} 12, 18 -- To: {Microscopy-at-Microscopy.com} 12, 18 -- Message-ID: {C358A4E2.14B6A%PWebster-at-hei.org} 12, 18 -- Thread-Topic: Osmium ignition 12, 18 -- Thread-Index: AcgiPnI9sKHvco4xEdycaQANk7Zh7g== 12, 18 -- Mime-version: 1.0 12, 18 -- Content-type: text/plain; 12, 18 -- charset="US-ASCII" 12, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
Have a requests for the purchase of a new XRD system. Would any on this list have any expeience in purchase of a new barebones system that's really cheap (economical). Application is for XRD identification of mineral powders.
Thanks
Roy Beavers
Southern Methodist University Department of Geological Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 7, 23 -- From rbeavers-at-mail.smu.edu Thu Nov 8 14:58:38 2007 7, 23 -- Received: from s31xub.systems.smu.edu (s31xub.systems.smu.edu [129.119.70.73]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA8Kwchm003238 7, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Nov 2007 14:58:38 -0600 7, 23 -- Received: from s31xe7.systems.smu.edu ([129.119.70.140]) by s31xub.systems.smu.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 23 -- Thu, 8 Nov 2007 14:58:33 -0600 7, 23 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="us-ascii" 7, 23 -- Subject: Question on XRD systems 7, 23 -- Date: Thu, 8 Nov 2007 14:58:33 -0600 7, 23 -- Message-ID: {F8AF6BF6CD1CC040AF35B0C2D1680BBB021A716A-at-s31xe7.systems.smu.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- Thread-Topic: Question on XRD systems 7, 23 -- Thread-index: AcgiSh9vvQkl+nlpR8q9I89AGCHUtQ== 7, 23 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 7, 23 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 08 Nov 2007 20:58:33.0365 (UTC) FILETIME=[1FA15450:01C8224A] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lA8Kwchm003238 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both erin-at-aaisolutions.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: erin-at-aaisolutions.com Name: Erin Curry
Organization: Alliance Analytical
Title-Subject: [Filtered] Confocal Microscope available
Question: We have a Leica TCS SL confocal microscope available. Interested parties can please contact us at the email address erin-at-aaisolutions.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both linda_beck-at-fws.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: linda_beck-at-fws.gov Name: Linda Beck
Organization: USFWS-Bozeman Fish Technology Center
Title-Subject: [Filtered] 3D image analysis preparations
Question: Dear histo prep experts:
Background:
We our analyzing sturgeon hearts to obtain quantitative values of lymphatic tissue verses cardiac muscle in a give whole heart. Our approach was to section 5 micron sections 10 times throughout the whole embedded heart and stain with H&E. The problem we are having is how to line up the tissue for a great 3-D image. A suggestion of inserting 3 teflon rods through the tissue for orientation was given to my collaborator. In addition, to cut 25 micron sections verses the 5 microns.
My questions for you to ponder are: 1. Do these teflon rods exist? Is this a good option or is there something else we can do for better orientation of tissue for 3D imaging? What company could I order the needed materials from?
2. If we cut these sections in 25 micron sections will we still be able to coverslip using a standard coverslip for 5 micron sections without having airbubble issues?
Thank you for any comments or suggestions!! I look forward to hearing from you.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dainis-at-red5wood as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dainis-at-red5wood Name: Dainis Dauksta
Title-Subject: [Filtered] Leica 'C' prism
Question: Does anyone have a surplus, maybe gathering dust, Leica 'C' prism for IC please? Leica part no. (11)555009 Thanks! Dainis
As we need to make room, I am on the way to loan for a long terme an Zeiss Ultraphot II optical microscope to a local optics intrument museum, I need to know an insurance value of it.
The ultraphot is well described on http://www.the-ultraphot-shop.org.uk/ and will be given with epi and dia illumination systemes, and a set of 5 BF/DF objectives. It is dated from 1958, and is in good condition, after some work to clean it.
Does someone have an idee of such a value.
Thanks for advices.
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Very interesting indeed! How was the OS crystal handled prior to being put into the Falcon Tube? Was it dumped from a freshly opened ampoule?
Best,
Al Coritz Technical Engineer Electron Microscopy Sciences www.emsdiasum.com
-----Original Message----- X-from: PWebster-at-hei.org [mailto:PWebster-at-hei.org] Sent: Thursday, November 08, 2007 2:35 PM To: Sampleprep-at-earthlink.net
Dear All,
As a cautionary tale I relate an incident that happened in our lab recently.
Someone placed 0.25g of osmium tetroxide crystals into a dry, clean 50ml polypropylene Falcon tube and it immediately burst into flames.
The inside of the tube turned black and the crystals disappeared. Luckily she was working in the chemical hood so no harm was done.
As I have been working with osmium tetroxide for many years with no problem, it was a surprise to me that this chemical could burst into flames so easily. Can anyone offer any insight on why this might happen?
One reason why this may not have happened to me is that I always follow the good advice of adding solid to liquid.
Regards,
Paul Webster.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
==============================Original Headers============================== 12, 18 -- From PWebster-at-hei.org Thu Nov 8 13:34:59 2007 12, 18 -- Received: from hi0sml1.hei.org (House-Ear-Institute-1114861.cust-rtr.pacbell.net [71.136.31.242]) 12, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA8JYwEJ019039 12, 18 -- for {Microscopy-at-Microscopy.com} ; Thu, 8 Nov 2007 13:34:59 -0600 12, 18 -- Received: from 10.10.42.170 ([10.10.42.170]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 12, 18 -- Thu, 8 Nov 2007 19:34:58 +0000 12, 18 -- User-Agent: Microsoft-Entourage/11.3.6.070618 12, 18 -- Date: Thu, 08 Nov 2007 11:34:58 -0800 12, 18 -- Subject: Osmium ignition 12, 18 -- From: "Webster, Paul" {PWebster-at-hei.org} 12, 18 -- To: {Microscopy-at-Microscopy.com} 12, 18 -- Message-ID: {C358A4E2.14B6A%PWebster-at-hei.org} 12, 18 -- Thread-Topic: Osmium ignition 12, 18 -- Thread-Index: AcgiPnI9sKHvco4xEdycaQANk7Zh7g== 12, 18 -- Mime-version: 1.0 12, 18 -- Content-type: text/plain; 12, 18 -- charset="US-ASCII" 12, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 25, 26 -- From sampleprep-at-earthlink.net Fri Nov 9 08:04:20 2007 25, 26 -- Received: from elasmtp-mealy.atl.sa.earthlink.net (elasmtp-mealy.atl.sa.earthlink.net [209.86.89.69]) 25, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA9E4KiT021463 25, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Nov 2007 08:04:20 -0600 25, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 25, 26 -- s=dk20050327; d=earthlink.net; 25, 26 -- b=MvNmKSlJvm6EbmIpdAvwiknlMPaAJxmlnWcsBu0HlQmYXvbz+08gK1oh9/tYR41J; 25, 26 -- h=Received:Reply-To:From:To:Cc:References:Subject:Date:Organization:Message-ID:X-Mailer:X-MimeOLE:Thread-Index:In-Reply-To:X-ELNK-Trace:X-Originating-IP; 25, 26 -- Received: from [151.197.210.127] (helo=PC226165571919) 25, 26 -- by elasmtp-mealy.atl.sa.earthlink.net with asmtp (Exim 4.34) 25, 26 -- id 1IqUT5-0004HR-Mq; Fri, 09 Nov 2007 09:04:19 -0500 25, 26 -- Reply-To: {sampleprep-at-earthlink.net} 25, 26 -- From: "Al Coritz" {sampleprep-at-earthlink.net} 25, 26 -- To: {PWebster-at-hei.org} 25, 26 -- Cc: {Microscopy-at-microscopy.com} 25, 26 -- References: {200711081935.lA8JZPsO019724-at-ns.microscopy.com} 25, 26 -- Subject: RE: [Microscopy] Osmium ignition 25, 26 -- Date: Fri, 9 Nov 2007 09:04:21 -0500 25, 26 -- Organization: Electron Microscopy Sciences 25, 26 -- Message-ID: {001801c822d9$6d49f470$b401a8c0-at-PC226165571919} 25, 26 -- X-Mailer: Microsoft Office Outlook 11 25, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 25, 26 -- Thread-Index: AcgiPoJVmH34jbb+QJux3LW9XJWxzAAmnImA 25, 26 -- In-Reply-To: {200711081935.lA8JZPsO019724-at-ns.microscopy.com} 25, 26 -- X-ELNK-Trace: 3b6fa8abc6003d9c3fea7eaafe4be1dd239a348a220c2609f4500a230af130b0f9998ddc0b8ba197a2d4e88014a4647c350badd9bab72f9c350badd9bab72f9c 25, 26 -- X-Originating-IP: 151.197.210.127 ==============================End of - Headers==============================
Hi Linda You could use AutoAligner to align the images after the fact. I have had very good luck with it for 3D reconstruction of serial TEM sections. David
On Nov 9, 2007, at 12:35 AM, linda_beck-at-fws.gov wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } ---------------------------------------------------------------------- } ----- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both linda_beck-at-fws.gov as well as the MIcroscopy } Listserver } ---------------------------------------------------------------------- } ----- } } Email: linda_beck-at-fws.gov } Name: Linda Beck } } Organization: USFWS-Bozeman Fish Technology Center } } Title-Subject: [Filtered] 3D image analysis preparations } } Question: Dear histo prep experts: } } Background: } } We our analyzing sturgeon hearts to obtain quantitative values of } lymphatic tissue verses cardiac muscle in a give whole heart. Our } approach was to section 5 micron sections 10 times throughout the } whole embedded heart and stain with H&E. The problem we are having } is how to line up the tissue for a great 3-D image. A suggestion of } inserting 3 teflon rods through the tissue for orientation was given } to my collaborator. In addition, to cut 25 micron sections verses } the 5 microns. } } My questions for you to ponder are: } 1. Do these teflon rods exist? Is this a good option or is there } something else we can do for better orientation of tissue for 3D } imaging? What company could I order the needed materials from? } } 2. If we cut these sections in 25 micron sections will we still be } able to coverslip using a standard coverslip for 5 micron sections } without having airbubble issues? } } Thank you for any comments or suggestions!! I look forward to } hearing from you. } } Cheers, } lin } } } Login Host: 65.121.112.98 } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== } 13, 11 -- From zaluzec-at-microscopy.com Fri Nov 9 01:30:26 2007 } 13, 11 -- Received: from [10.106.3.197] (msdvpn8.msd.anl.gov } [130.202.238.72]) } 13, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id lA97UMjT017375 } 13, 11 -- for {microscopy-at-microscopy.com} ; Fri, 9 Nov 2007 } 01:30:24 -0600 } 13, 11 -- Mime-Version: 1.0 } 13, 11 -- Message-Id: {p06240805c359bcfcbf5f-at-[10.106.3.197]} } 13, 11 -- Date: Fri, 9 Nov 2007 08:30:21 +0100 } 13, 11 -- To: microscopy-at-microscopy.com } 13, 11 -- From: linda_beck-at-fws.gov (by way of MicroscopyListserver) } 13, 11 -- Subject: viaWWW: 3D image analysis preparations } 13, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers============================== }
==============================Original Headers============================== 5, 22 -- From Elliott-at-arizona.edu Fri Nov 9 12:14:52 2007 5, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA9IEqDR013922 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 9 Nov 2007 12:14:52 -0600 5, 22 -- Received: from gandalfs_amavis (amavis9.email.arizona.edu [10.0.0.237]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 3C82B3B1362 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 9 Nov 2007 11:14:52 -0700 (MST) 5, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 2232D3B1345 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 9 Nov 2007 11:14:50 -0700 (MST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 5, 22 -- In-Reply-To: {200711090735.lA97Z1HO031288-at-ns.microscopy.com} 5, 22 -- References: {200711090735.lA97Z1HO031288-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {EA576140-ACEB-471C-9F09-EDD72A484A09-at-arizona.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: David Elliott {Elliott-at-arizona.edu} 5, 22 -- Subject: Re: [Microscopy] viaWWW: 3D image analysis preparations 5, 22 -- Date: Fri, 9 Nov 2007 11:14:49 -0700 5, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 22 -- X-Mailer: Apple Mail (2.752.2) 5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
On Nov 8, 2007, at 11:35 AM, PWebster-at-hei.org wrote:
} As a cautionary tale I relate an incident that happened in our lab } recently. } } Someone placed 0.25g of osmium tetroxide crystals into a dry, clean } 50ml } polypropylene Falcon tube and it immediately burst into flames. } } The inside of the tube turned black and the crystals disappeared. } Luckily } she was working in the chemical hood so no harm was done. } } As I have been working with osmium tetroxide for many years with no } problem, } it was a surprise to me that this chemical could burst into flames so } easily. Can anyone offer any insight on why this might happen? } } One reason why this may not have happened to me is that I always } follow the } good advice of adding solid to liquid.
Dear Paul, OsO4 is a strong oxidizing agent, and polypropylene will burn, so I am not too surprised. The wisdom of adding solid to liquid is exemplified here, since in that case the concentration of OsO4 is lessened, and furthermore the mass of water will counteract any temperature increase caused when the polypropylene is oxidized. Both effects slow down the oxidation reaction. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 5, 22 -- From tivol-at-caltech.edu Fri Nov 9 12:43:40 2007 5, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA9IhdV5027344 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Nov 2007 12:43:39 -0600 5, 22 -- Received: from water-dog.its.caltech.edu (water-dog [192.168.1.26]) 5, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id 51E1013DAD 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Nov 2007 10:43:36 -0800 (PST) 5, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 5, 22 -- by wood-ox.its.caltech.edu (Postfix) with ESMTP id 3BFD813DCA 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Nov 2007 10:42:59 -0800 (PST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v752.3) 5, 22 -- In-Reply-To: {200711081935.lA8JZBYO019189-at-ns.microscopy.com} 5, 22 -- References: {200711081935.lA8JZBYO019189-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {B0ED84AF-6F3B-444A-81F7-7972D990D19B-at-caltech.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 22 -- Subject: Re: [Microscopy] Osmium ignition 5, 22 -- Date: Fri, 9 Nov 2007 10:43:15 -0800 5, 22 -- To: microscopy-at-msa.microscopy.com 5, 22 -- X-Mailer: Apple Mail (2.752.3) 5, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.4.5 ==============================End of - Headers==============================
I've always first rinsed the ampule in hot water and then distilled water. The osmium tetroxide crystals would melt and then I'd allow them to recrystallize along the sides of the ampule. Next we'd place the ampule inside a glass-stoppered reagent bottle (100 ml) and shake the bottle vigorously to break the ampule. Any solutions, water, buffers, etc. would be added to the reagent bottle. Lastly we'd sonicate the solution for at least 30 minutes. Method of Bill Wergin and the Wisconsin gang. Always with safety precautions: gloves, eye protection, fume hood, etc. No problems.
Bruce F. Ingber, Biologist Electron Microscopy USDA-ARS, SRRC CSQ-EM 1100 Robert E. Lee Blvd. New Orleans, LA 70124 bingber-at-srrc.ars.usda.gov 504-286-4270 desk phone 504-782-6323 cell
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On Nov 8, 2007, at 11:35 AM, PWebster-at-hei.org wrote:
} As a cautionary tale I relate an incident that happened in our lab } recently. } } Someone placed 0.25g of osmium tetroxide crystals into a dry, clean } 50ml } polypropylene Falcon tube and it immediately burst into flames. } } The inside of the tube turned black and the crystals disappeared. } Luckily } she was working in the chemical hood so no harm was done. } } As I have been working with osmium tetroxide for many years with no } problem, } it was a surprise to me that this chemical could burst into flames so } easily. Can anyone offer any insight on why this might happen? } } One reason why this may not have happened to me is that I always } follow the } good advice of adding solid to liquid.
Dear Paul, OsO4 is a strong oxidizing agent, and polypropylene will burn, so I am not too surprised. The wisdom of adding solid to liquid is exemplified here, since in that case the concentration of OsO4 is lessened, and furthermore the mass of water will counteract any temperature increase caused when the polypropylene is oxidized. Both effects slow down the oxidation reaction. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 5, 22 -- From tivol-at-caltech.edu Fri Nov 9 12:43:40 2007 5, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA9IhdV5027344 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Nov 2007 12:43:39 -0600 5, 22 -- Received: from water-dog.its.caltech.edu (water-dog [192.168.1.26]) 5, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id 51E1013DAD 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Nov 2007 10:43:36 -0800 (PST) 5, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 5, 22 -- by wood-ox.its.caltech.edu (Postfix) with ESMTP id 3BFD813DCA 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 9 Nov 2007 10:42:59 -0800 (PST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v752.3) 5, 22 -- In-Reply-To: {200711081935.lA8JZBYO019189-at-ns.microscopy.com} 5, 22 -- References: {200711081935.lA8JZBYO019189-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {B0ED84AF-6F3B-444A-81F7-7972D990D19B-at-caltech.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 22 -- Subject: Re: [Microscopy] Osmium ignition 5, 22 -- Date: Fri, 9 Nov 2007 10:43:15 -0800 5, 22 -- To: microscopy-at-msa.microscopy.com 5, 22 -- X-Mailer: Apple Mail (2.752.3) 5, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.4.5 ==============================End of - Headers==============================
==============================Original Headers============================== 12, 20 -- From bingber-at-srrc.ars.usda.gov Fri Nov 9 13:25:38 2007 12, 20 -- Received: from srrc.ars.usda.gov (marconi.srrc.ars.usda.gov [199.133.86.11]) 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lA9JPbRE009192 12, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 9 Nov 2007 13:25:38 -0600 12, 20 -- Received: from (unknown [199.133.86.11]) by DA32USMOKC1_AVS01.usda.gov with smtp 12, 20 -- id 4b3f_8cedd7ce_8ef9_11dc_9c72_001143d22ff1; 12, 20 -- Fri, 09 Nov 2007 19:25:38 +0000 12, 20 -- Received: from SRRCDOM-MTA by srrc.ars.usda.gov 12, 20 -- with Novell_GroupWise; Fri, 09 Nov 2007 13:25:36 -0600 12, 20 -- Message-Id: {s7345fd0.094-at-srrc.ars.usda.gov} 12, 20 -- X-Mailer: Novell GroupWise Internet Agent 6.0.4 12, 20 -- Date: Fri, 09 Nov 2007 13:25:04 -0600 12, 20 -- From: "Bruce Ingber" {bingber-at-srrc.ars.usda.gov} 12, 20 -- To: {tivol-at-caltech.edu} , {Microscopy-at-MSA.Microscopy.Com} 12, 20 -- Subject: [Microscopy] Re: Osmium ignition 12, 20 -- Mime-Version: 1.0 12, 20 -- Content-Type: text/plain; charset=US-ASCII 12, 20 -- Content-Disposition: inline 12, 20 -- Content-Transfer-Encoding: 8bit 12, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lA9JPbRE009192 ==============================End of - Headers==============================
I really hate to have the glass fragments in my osmium stocks. I pour a few mls of liquid nitrogen into a 50 ml disposable beaker and then add the osmium ampule. The difference in contraction rates between the glass and osmium crystal results in it popping of the glass and no longer being sticky. I then snap the top off the ampule and pour the crystals into a water filled bottle. If you use a stir bar to mix, it goes in solution within an hour. This is easy to see since there are no glass bits that look like osmium crystals. Naturally we do all of the above steps in the fume hood. You don't want to add liquid nitrogen to an open or cracked vial since it could expand rapidly and explode but if you add the ampule to only a few mls, it rapidly turns to gas and after 2 minutes or so, you can remove the still cold but no longer nitrogen immersed vial.
. At 01:26 PM 11/09/07, bingber-at-srrc.ars.usda.gov wrote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
Hi, I am looking for a new or used grabBit PCI framegrabber for MegaView II camera system. If you have one and do not use it any more, could you please contact me?
I am also looking for a used MegaView III camera block or the whole system.
Thank you. Ping
-- Ping Li, Ph.D. Director, Scientific Imaging Suite Department of Biology Dalhousie University Halifax, NS B3H 4J1 Canada
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Email: hubner-at-iod.krakow.pl Name: Krzysztof Jan H¸bner
Organization: Foundry Research Institute KrakÛw
Title-Subject: [Filtered] conference STERMAT 2008
Question:
VIII International Conference on Stereology and Image Analysis in Materials Science STERMAT 2008
Zakopane, Poland, 2-6 September, 2008 link http://m7.mech.pk.edu.pl/~stermat/ Best regards
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Email: vamsi.88-at-gmail.com Name: Talla Vamsi
Organization: IIT Guwahati
Title-Subject: [Filtered] Color microscopy
Question: Hi, I have a very basic doubt in optical microscopy.I wish to analyze a color photographic film under a microscope in transmission mode. My aim to analyze the smallest spot possible for colors namely white,blue red and green. Hence if I know the NA of the microscope the resolution is given by .6*lambda/NA. So will the minimum spot for every color be different and if different then how much?? Is it possible to make the minimum resolution 200nm for every color in optical microscopy??? Your help will immensely help me in project so reply as soon a possible.
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Title-Subject: [Filtered] Thanks so much EDX plants/seeds
Question: Experts, Thanks so much for all the information. This will be quite a challenge, if at all possible. As was offered by some of you, I will contact you offline and report back here any worthwhile results. Regards and much appreciation, Winnie
Daniel Salamon asked about analyzing how well ordered is a group of particles (seen in a TEM image) that nominally forms a hexagonal array. He supposed that FFT analysis might be useful. REPLY: Although FFT might be useful, my own preference is for real-space analysis. We developed a commercial software (DiscTrack Plus) for the optical disc industry that measures the spacing of columns in an array ("pitch") and the spacing of bumps within those columns. By presenting results in real-space, the software directly points to where the pattern is good and where it is bad. And statistical measures of variation are given in real-space units, e.g. standard deviation of column pitch = some number of nm. You may find the following link interesting: www.asmicro.com/download_files/2D_NanoMetrology_(ar1267).pdf
I suspect that our software would be useful here and I invite submission of sample images. Please contact me offline for details. regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
----- Original Message ----- From: Daniel.Salamon-at-nrc-cnrc.gc.ca To: donc-at-asmicro.com Sent: Wednesday, November 07, 2007 4:42 PM Subject: [a] [Microscopy] particle order analysis Hello everyone,
Thank you in advance for your help.
I have a series of images of particles arranged in a hexagonal pattern on a substrate. The particles can be either: - arranged in a perfectly hexagonal pattern or; - very close to a hexagonal pattern, but not perfect; - quite disordered but somewhat hexagonal pattern; - completely disordered;
Can anyone point me to a technique (description or literature) - probably FFT - to properly characterize how well the particles fit the intended order (hexagonal)? It is obvious from the images, but would be nice to put a number on it.
Thank you, Daniel
Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC 11421 Saskatchewan Dr. Edmonton, AB. T6G 2M9
Phone: (780) 641-1663 Fax: (780) 641-1601
==============================Original Headers============================== 12, 25 -- From donc-at-asmicro.com Sun Nov 11 21:55:09 2007 12, 25 -- Received: from smtp108.sbc.mail.re2.yahoo.com (smtp108.sbc.mail.re2.yahoo.com [68.142.229.97]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lAC3t8VI006995 12, 25 -- for {microscopy-at-microscopy.com} ; Sun, 11 Nov 2007 21:55:09 -0600 12, 25 -- Received: (qmail 14826 invoked from network); 12 Nov 2007 03:55:08 -0000 12, 25 -- Received: from unknown (HELO asm15) (asmicro-at-sbcglobal.net-at-68.58.145.13 with login) 12, 25 -- by smtp108.sbc.mail.re2.yahoo.com with SMTP; 12 Nov 2007 03:55:08 -0000 12, 25 -- X-YMail-OSG: 7u95j2cVM1kWfQWhZO5fAx6Fv_w0yURa28wtwPkTgGdj2ZxIErPteTwI_WI49JX5U8a5zXniJ2eWhGWYSkKKMg.kLALxVz_bkxa1Va44vyMoIf4KwPI- 12, 25 -- Message-ID: {004001c824df$c0224f00$0302a8c0-at-asm15} 12, 25 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 12, 25 -- To: {Daniel.Salamon-at-nrc-cnrc.gc.ca} , 12, 25 -- "Microscopy List" {microscopy-at-microscopy.com} 12, 25 -- References: {200711072142.lA7Lg1Ox013819-at-ns.microscopy.com} 12, 25 -- Subject: Re: [a] [Microscopy] particle order analysis 12, 25 -- Date: Sun, 11 Nov 2007 22:53:54 -0500 12, 25 -- MIME-Version: 1.0 12, 25 -- Content-Type: text/plain; 12, 25 -- format=flowed; 12, 25 -- charset="iso-8859-1"; 12, 25 -- reply-type=original 12, 25 -- Content-Transfer-Encoding: 7bit 12, 25 -- X-Priority: 3 12, 25 -- X-MSMail-Priority: Normal 12, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 12, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
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Email: mgriffin-at-sas.samsung.com Name: Mary Griffin
Organization: Samsung Austin Semiconductor
Title-Subject: [Filtered] Job Opportunity at Samsung Austin Semiconductor
Question: Samsung Austin Semiconductor is seeking a TEM engineer to join its Defect and Particle team. The successful candidate will be dedicated to TEM operation and data reporting, will be engaged in root cause investigation on manufacturing excursions and on defect generation for fab yield improvement, as well as driving to cut down analysis TAT. Responsibilities include: ï Manage daily TEM Microscope imaging ï Perform TEM imaging and summary write ups ï Oversee TEM sample prep and provide feedback ï Work with unit process and product technology engineers to resolve device and in line issues Qualified candidates will possess: ï MS or Ph.D. in EE, ChemE, Physics, or Material Science, or equivalent ï 3+ years relevant experience ï TEM microscope hands on operation ï Knowledge of DRAM device technology and fabrication process ï FLASH device knowledge
Please send resumes or questions to Mary Griffin at mgriffin-at-sas.samsung.com
Strange query fot list server. We have found a specimen rod made by Jeol for 4 grids. It has a strange clipping mechanism that were not sure how it works. It seems to take circlips and come with a clip injector. We found it in a catalogue but there is no mention of how to use it. So any advice would be most appreciated as we haven't found anything on the net. On the back of the rod is "no 30" and "MSH 10". So far it has baffled all of us at the uni of Bristol who have never seen one and also a Jeol engineer with 30 years experience. Thanks in advance. John
==============================Original Headers============================== 1, 27 -- From john.mitchels-at-gmail.com Tue Nov 13 04:12:42 2007 1, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.189]) 1, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lADACgYq019380 1, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Nov 2007 04:12:42 -0600 1, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so1803119rvb 1, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Nov 2007 02:12:41 -0800 (PST) 1, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 27 -- d=gmail.com; s=beta; 1, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 1, 27 -- bh=O21xHOskMbgeGeoEzbSmdyr5eGOPOBP3DTEjp8InVx8=; 1, 27 -- b=rZzNJBZZt4IQVAaygOSBZc1+sH2mo4RlykwOnQzUt6lwtyxJR7TrnUx6DTGCXu8GEx2chSe0i/EemcwuMZBcQHSvqYjxlJqbZhBGj625SxPj0ZYhdDdZCdCEnKJoCSJikrAIToUpfu1fcB0PGj67bKxcmtKOo2q3EDTanuuMMCk= 1, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 1, 27 -- d=gmail.com; s=beta; 1, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 1, 27 -- b=SA53d1mkIpPJ0ZA7AtYvirtgb7BUreVpsCgmr+oQCM60V96Vc50v9qAPFjBiCtPaSpkDbKT2NZKci0fCgvYueVlhZHwQ76rac5VfbsAx5+5ztZVI9UTGulgDjHG59Ro+s7hyVdGchdOqRiSEc4ojGOD9MJpcfwbUfUUlr0J9Tco= 1, 27 -- Received: by 10.142.102.5 with SMTP id z5mr1670838wfb.1194948761703; 1, 27 -- Tue, 13 Nov 2007 02:12:41 -0800 (PST) 1, 27 -- Received: by 10.142.142.2 with HTTP; Tue, 13 Nov 2007 02:12:41 -0800 (PST) 1, 27 -- Message-ID: {1b3cf7c30711130212t1b4a38ebkf8a330dfebe42e54-at-mail.gmail.com} 1, 27 -- Date: Tue, 13 Nov 2007 10:12:41 +0000 1, 27 -- From: "John Mitchels" {john.mitchels-at-gmail.com} 1, 27 -- To: Microscopy-at-microscopy.com 1, 27 -- Subject: TEM: Strange Sample Rod 1, 27 -- MIME-Version: 1.0 1, 27 -- Content-Type: text/plain; charset=ISO-8859-1 1, 27 -- Content-Transfer-Encoding: 7bit 1, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
I may be having a mental block on this one, but I can't remember (or never knew!) the alignment parameter that would address the following problem:
In our TEM, especially at high mags, using the coarse focus control causes the beam to oscillate away from the screen center. The image of the specimen does not move significantly so I am not talking about alignment with the voltage or current centering. The condenser and objective apertures are aligned, as verified by sweeping the beam with the brightness control.
In short, the beam moves around during focusing, but the specimen's image does not. The problem is that we constantly have to recenter the beam during high mag work to maintain illumination.
Any thoughts from the Collective on what I'm missing?
Thanks, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 8, 23 -- From TindallR-at-missouri.edu Tue Nov 13 08:28:19 2007 8, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lADESJLB007205 8, 23 -- for {microscopy-at-microscopy.com} ; Tue, 13 Nov 2007 08:28:19 -0600 8, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 23 -- Tue, 13 Nov 2007 08:28:19 -0600 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="us-ascii" 8, 23 -- Subject: TEM: Alignment question 8, 23 -- Date: Tue, 13 Nov 2007 08:27:40 -0600 8, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BEDF-at-UM-XMAIL08.um.umsystem.edu} 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- Thread-Topic: TEM: Alignment question 8, 23 -- thread-index: AcgmAVllVKeou4oKT2eJgFw3JBC5Aw== 8, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 8, 23 -- To: {microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 13 Nov 2007 14:28:19.0223 (UTC) FILETIME=[6FC78A70:01C82601] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lADESJLB007205 ==============================End of - Headers==============================
I had a problem with the beam moving a long time ago and someone suggested that I clean the specimen holder and after that the problem went away. Maybe it will work for you.
Pat
Patricia Stranen Connelly Lead Technologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov
} Dear Listers, } } I may be having a mental block on this one, but I can't remember (or } never knew!) the alignment parameter that would address the following } problem: } } In our TEM, especially at high mags, using the coarse focus control } causes the beam to oscillate away from the screen center. The image of } the specimen does not move significantly so I am not talking about } alignment with the voltage or current centering. The condenser and } objective apertures are aligned, as verified by sweeping the beam with } the brightness control. } } In short, the beam moves around during focusing, but the specimen's } image does not. The problem is that we constantly have to recenter the } beam during high mag work to maintain illumination. } } Any thoughts from the Collective on what I'm missing? } } Thanks, } Randy Tindall } Senior EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu
==============================Original Headers============================== 9, 24 -- From connellyps-at-nhlbi.nih.gov Tue Nov 13 09:13:11 2007 9, 24 -- Received: from NIHCESSMTP2.hub.nih.gov (nihcessmtp2.hub.nih.gov [128.231.90.116]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lADFD7IY020813 9, 24 -- for {microscopy-at-microscopy.com} ; Tue, 13 Nov 2007 09:13:10 -0600 9, 24 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 9, 24 -- Tue, 13 Nov 2007 10:12:58 -0500 9, 24 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.165]) with Microsoft Exchange Server HTTP-DAV ; 9, 24 -- Tue, 13 Nov 2007 15:12:56 +0000 9, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618 9, 24 -- Date: Tue, 13 Nov 2007 10:10:54 -0500 9, 24 -- Subject: Re: [Microscopy] TEM: Alignment question 9, 24 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 9, 24 -- To: Randy Tindall {TindallR-at-missouri.edu} 9, 24 -- CC: {microscopy-at-microscopy.com} 9, 24 -- Message-ID: {C35F28AE.FF7%connellyps-at-nhlbi.nih.gov} 9, 24 -- Thread-Topic: [Microscopy] TEM: Alignment question 9, 24 -- Thread-Index: AcgmB2KLoPay0pH6EdyPWwANk2Yv1A== 9, 24 -- In-Reply-To: {200711131434.lADEYfc4015610-at-ns.microscopy.com} 9, 24 -- Mime-version: 1.0 9, 24 -- Content-type: text/plain; 9, 24 -- charset="ISO-8859-1" 9, 24 -- X-OriginalArrivalTime: 13 Nov 2007 15:12:58.0320 (UTC) FILETIME=[ACA54D00:01C82607] 9, 24 -- Content-Transfer-Encoding: 8bit 9, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lADFD7IY020813 ==============================End of - Headers==============================
This could be a charging issue - if so cleaning the spec rod would sort it.
And a plea to Listers: I have a problem with the focus of our Philips CM10:
When the image is static using the wobbler, the image looks distinctly out of focus (though it looks OK with a holey carbon film), and is greatly improved by ignoring the wobble when focusing the image.
Can anybody advise if there is an optimum under focus adjustment available on the CM10 (as there was/is with Jeol TEM's), or a means of adjusting (or reconnecting) the wobbler to co-relate with the image focus.
Any advice gratefully received.
Thanks in advance,
Alastair.
Alastair McKinnon Histology & EM Facility Manager University of Aberdeen, Institute of Medical Science Foresterhill, Aberdeen, AB25 2ZD tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk www.abdn.ac.uk/ims/h-em -----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: 13 November 2007 14:34 To: Mckinnon, Alastair D.
Dear Listers,
I may be having a mental block on this one, but I can't remember (or never knew!) the alignment parameter that would address the following problem:
In our TEM, especially at high mags, using the coarse focus control causes the beam to oscillate away from the screen center. The image of the specimen does not move significantly so I am not talking about alignment with the voltage or current centering. The condenser and objective apertures are aligned, as verified by sweeping the beam with the brightness control.
In short, the beam moves around during focusing, but the specimen's image does not. The problem is that we constantly have to recenter the beam during high mag work to maintain illumination.
Any thoughts from the Collective on what I'm missing?
Thanks, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 8, 23 -- From TindallR-at-missouri.edu Tue Nov 13 08:28:19 2007 8, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lADESJLB007205 8, 23 -- for {microscopy-at-microscopy.com} ; Tue, 13 Nov 2007 08:28:19 -0600 8, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 23 -- Tue, 13 Nov 2007 08:28:19 -0600 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="us-ascii" 8, 23 -- Subject: TEM: Alignment question 8, 23 -- Date: Tue, 13 Nov 2007 08:27:40 -0600 8, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BEDF-at-UM-XMAIL08.um.umsystem.edu} 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- Thread-Topic: TEM: Alignment question 8, 23 -- thread-index: AcgmAVllVKeou4oKT2eJgFw3JBC5Aw== 8, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 8, 23 -- To: {microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 13 Nov 2007 14:28:19.0223 (UTC) FILETIME=[6FC78A70:01C82601] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lADESJLB007205 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 24 -- From a.d.mckinnon-at-abdn.ac.uk Tue Nov 13 10:03:20 2007 24, 24 -- Received: from mailhub3.abdn.ac.uk (mailhub3.abdn.ac.uk [139.133.7.13]) 24, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lADG3JXE001914 24, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Nov 2007 10:03:20 -0600 24, 24 -- Received: from ew-mail-a.uoa.abdn.ac.uk ([139.133.15.20] helo=VMAIL1.uoa.abdn.ac.uk) 24, 24 -- by mailhub3.abdn.ac.uk with esmtp (Exim 4.52) 24, 24 -- id 1IryEQ-0006qL-Vg; Tue, 13 Nov 2007 16:03:19 +0000 24, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 24, 24 -- Content-class: urn:content-classes:message 24, 24 -- MIME-Version: 1.0 24, 24 -- Content-Type: text/plain; 24, 24 -- charset="us-ascii" 24, 24 -- Subject: RE: [Microscopy] TEM: Alignment question 24, 24 -- Date: Tue, 13 Nov 2007 16:01:20 -0000 24, 24 -- Message-ID: {DE488CE26EE0F944BD1A85702EE64BCAFA27A6-at-VMAIL1.uoa.abdn.ac.uk} 24, 24 -- X-MS-Has-Attach: 24, 24 -- X-MS-TNEF-Correlator: 24, 24 -- Thread-Topic: [Microscopy] TEM: Alignment question 24, 24 -- thread-index: AcgmDAOHPj5OUPjMQAm13WBCWtQwkAAAM99g 24, 24 -- From: "Mckinnon, Alastair D." {a.d.mckinnon-at-abdn.ac.uk} 24, 24 -- To: {TindallR-at-missouri.edu} 24, 24 -- Cc: {Microscopy-at-microscopy.com} 24, 24 -- Content-Transfer-Encoding: 8bit 24, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lADG3JXE001914 ==============================End of - Headers==============================
Hi Randy, I would consider the beam shift (or tilt too) and its compensators. Some of the old TEMs even have a beam compensators (X and Y) on the upper column, such as old JEM TEMs, which compensate the beam shifts during changing the focus of obj-lens in a large range, such as through focus series. Long
Long Li ------------------------------------------- University of Pittsburgh longli_TEM-at-hotmail.com
Long Li ------------------------------------------- University of Pittsburgh longli_TEM-at-hotmail.com
-----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: Tuesday, November 13, 2007 9:32 AM To: Longli_tem-at-hotmail.com
Dear Listers,
I may be having a mental block on this one, but I can't remember (or never knew!) the alignment parameter that would address the following problem:
In our TEM, especially at high mags, using the coarse focus control causes the beam to oscillate away from the screen center. The image of the specimen does not move significantly so I am not talking about alignment with the voltage or current centering. The condenser and objective apertures are aligned, as verified by sweeping the beam with the brightness control.
In short, the beam moves around during focusing, but the specimen's image does not. The problem is that we constantly have to recenter the beam during high mag work to maintain illumination.
Any thoughts from the Collective on what I'm missing?
Thanks, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 8, 23 -- From TindallR-at-missouri.edu Tue Nov 13 08:28:19 2007 8, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lADESJLB007205 8, 23 -- for {microscopy-at-microscopy.com} ; Tue, 13 Nov 2007 08:28:19 -0600 8, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 23 -- Tue, 13 Nov 2007 08:28:19 -0600 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="us-ascii" 8, 23 -- Subject: TEM: Alignment question 8, 23 -- Date: Tue, 13 Nov 2007 08:27:40 -0600 8, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BEDF-at-UM-XMAIL08.um.umsystem.edu} 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- Thread-Topic: TEM: Alignment question 8, 23 -- thread-index: AcgmAVllVKeou4oKT2eJgFw3JBC5Aw== 8, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 8, 23 -- To: {microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 13 Nov 2007 14:28:19.0223 (UTC) FILETIME=[6FC78A70:01C82601] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lADESJLB007205 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 22 -- From longli_tem-at-hotmail.com Tue Nov 13 10:42:37 2007 18, 22 -- Received: from bay0-omc2-s34.bay0.hotmail.com (bay0-omc2-s34.bay0.hotmail.com [65.54.246.170]) 18, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lADGgbVG015416 18, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Nov 2007 10:42:37 -0600 18, 22 -- Received: from BAY127-DS1 ([65.55.132.28]) by bay0-omc2-s34.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.3959); 18, 22 -- Tue, 13 Nov 2007 08:42:35 -0800 18, 22 -- X-Originating-IP: [136.142.89.250] 18, 22 -- X-Originating-Email: [longli_tem-at-hotmail.com] 18, 22 -- Message-ID: {BAY127-DS11F8BA6CCDF1675EC6BFFE3800-at-phx.gbl} 18, 22 -- From: "Long Li" {longli_tem-at-hotmail.com} 18, 22 -- To: {Microscopy-at-microscopy.com} 18, 22 -- References: {200711131431.lADEVraB011604-at-ns.microscopy.com} 18, 22 -- In-Reply-To: {200711131431.lADEVraB011604-at-ns.microscopy.com} 18, 22 -- Subject: RE: [Microscopy] TEM: Alignment question 18, 22 -- Date: Mon, 12 Nov 2007 23:42:25 -0500 18, 22 -- MIME-Version: 1.0 18, 22 -- Content-Type: text/plain; charset="US-ASCII" 18, 22 -- Content-Transfer-Encoding: 7bit 18, 22 -- X-Mailer: Microsoft Office Outlook 12.0 18, 22 -- Thread-Index: AcgmAe/LsDNYhEItQuC4JR64rk98VgAUn/YQ 18, 22 -- Content-Language: en-us 18, 22 -- X-OriginalArrivalTime: 13 Nov 2007 16:42:35.0533 (UTC) FILETIME=[31B6E3D0:01C82614] ==============================End of - Headers==============================
I'm flying blind here. I have a question from a users about something don't know about.
He looks at nanoparticles. Recently he showed me a picture of some of his particles and asked about the location of the lattice lines.
The particles are Ti, about 2 nm in size on a plain carbon film. He looks at them at a HRTEM lab someplace else. His pictures show lots of background grain, but you can make out some areas that are denser and we assume that these are his particles. Associated with many of the particles are a series of parallel lines that look like lattice fringes, but they appear to be offset from the particle.
His question is why do these lines appear to be offset from the main image of the particle? I have a picture if you need to see it.
Any ideas?
Thanks
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. Visit http://www.aidslifecycle.org for more information about the ride.
==============================Original Headers============================== 10, 20 -- From jmkrupp-at-ucsc.edu Tue Nov 13 12:45:01 2007 10, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lADIj0rX032365 10, 20 -- for {microscopy-at-microscopy.com} ; Tue, 13 Nov 2007 12:45:01 -0600 10, 20 -- Received: from [128.114.125.121] (HELO ucsc.edu) 10, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 10, 20 -- with ESMTPS id 25964888 for microscopy-at-microscopy.com; Tue, 13 Nov 2007 10:45:00 -0800 10, 20 -- Received: by email-prod-fe-2.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 10, 20 -- with PIPE id 19056505; Tue, 13 Nov 2007 10:45:00 -0800 10, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 10, 20 -- Received: from [128.114.25.127] (account jmkrupp-at-ucsc.edu HELO [128.114.25.127]) 10, 20 -- by email-prod-fe-2.ucsc.edu (CommuniGate Pro SMTP 5.1.11) 10, 20 -- with ESMTPA id 19056494 for microscopy-at-microscopy.com; Tue, 13 Nov 2007 10:44:57 -0800 10, 20 -- Mime-Version: 1.0 10, 20 -- Message-Id: {p06230903c35f9ee5205e-at-[128.114.25.127]} 10, 20 -- Date: Tue, 13 Nov 2007 10:44:55 -0800 10, 20 -- To: microscopy-at-microscopy.com 10, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 10, 20 -- Subject: lattice fringes 10, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
We have a similar rod though ours takes 5 grids - same code MSH10 though ours is number 28. It was a specific design constructed by JEOL UK for rapidly looking at multiple grids - particularly of use when people here were scanning large numbers of virus preps though we have not used it for over a decade. The grids slip into the depressions and as you suggest a circlip is pushed in with the injector to hold it in place. The holder is inserted as normal and the grid selected by the movable rod in the handle (should have markers on for each grid position and lock in place). There should be a small dimple at the edge of each of the depressions holding the grids that allows you to use fine forceps to extract the circlip and recover the grid.
Hope this helps.
Ian
Ian Hallett Sensory and Consumer Science - Microscopy HortResearch, Mt Albert Research Centre Private Bag 92 169, Auckland Mail Centre Auckland 1142, New Zealand +64-9-815 4200 ext 7002
-----Original Message----- X-from: john.mitchels-at-gmail.com [mailto:john.mitchels-at-gmail.com] Sent: Tuesday, 13 November 2007 11:16 p.m. To: Ian Hallett
Strange query fot list server. We have found a specimen rod made by Jeol for 4 grids. It has a strange clipping mechanism that were not sure how it works. It seems to take circlips and come with a clip injector. We found it in a catalogue but there is no mention of how to use it. So any advice would be most appreciated as we haven't found anything on the net. On the back of the rod is "no 30" and "MSH 10". So far it has baffled all of us at the uni of Bristol who have never seen one and also a Jeol engineer with 30 years experience. Thanks in advance. John
==============================Original Headers============================== 1, 27 -- From john.mitchels-at-gmail.com Tue Nov 13 04:12:42 2007 1, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.189]) 1, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lADACgYq019380 1, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Nov 2007 04:12:42 -0600 1, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so1803119rvb 1, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Nov 2007 02:12:41 -0800 (PST) 1, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 27 -- d=gmail.com; s=beta; 1, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject: mime-version:content-type:content-transfer-encoding:content-disposition; 1, 27 -- bh=O21xHOskMbgeGeoEzbSmdyr5eGOPOBP3DTEjp8InVx8=; 1, 27 -- b=rZzNJBZZt4IQVAaygOSBZc1+sH2mo4RlykwOnQzUt6lwtyxJR7TrnUx6DTGCXu8GEx2chS e0i/EemcwuMZBcQHSvqYjxlJqbZhBGj625SxPj0ZYhdDdZCdCEnKJoCSJikrAIToUpfu1fcB 0PGj67bKxcmtKOo2q3EDTanuuMMCk= 1, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 1, 27 -- d=gmail.com; s=beta; 1, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:con tent-transfer-encoding:content-disposition; 1, 27 -- b=SA53d1mkIpPJ0ZA7AtYvirtgb7BUreVpsCgmr+oQCM60V96Vc50v9qAPFjBiCtPaSpkDbK T2NZKci0fCgvYueVlhZHwQ76rac5VfbsAx5+5ztZVI9UTGulgDjHG59Ro+s7hyVdGchdOqRi SEc4ojGOD9MJpcfwbUfUUlr0J9Tco= 1, 27 -- Received: by 10.142.102.5 with SMTP id z5mr1670838wfb.1194948761703; 1, 27 -- Tue, 13 Nov 2007 02:12:41 -0800 (PST) 1, 27 -- Received: by 10.142.142.2 with HTTP; Tue, 13 Nov 2007 02:12:41 -0800 (PST) 1, 27 -- Message-ID: {1b3cf7c30711130212t1b4a38ebkf8a330dfebe42e54-at-mail.gmail.com} 1, 27 -- Date: Tue, 13 Nov 2007 10:12:41 +0000 1, 27 -- From: "John Mitchels" {john.mitchels-at-gmail.com} 1, 27 -- To: Microscopy-at-microscopy.com 1, 27 -- Subject: TEM: Strange Sample Rod 1, 27 -- MIME-Version: 1.0 1, 27 -- Content-Type: text/plain; charset=ISO-8859-1 1, 27 -- Content-Transfer-Encoding: 7bit 1, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 12, 27 -- From IHallett-at-hortresearch.co.nz Tue Nov 13 13:47:10 2007 12, 27 -- Received: from hortresearch.co.nz (mscan.hortresearch.co.nz [202.36.134.15]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lADJl8CI013784 12, 27 -- for {microscopy-at-microscopy.com} ; Tue, 13 Nov 2007 13:47:09 -0600 12, 27 -- Message-ID: {0937aa66000321c5-at-hortresearch.co.nz} 12, 27 -- Received: from aklexf01.hort.net.nz ([10.16.1.14]) by hortresearch.co.nz ([192.168.8.15]) with SMTP (TREND IMSS SMTP Service 7.0) id 0937aa66000321c5 for {IHallett-at-hortresearch.co.nz} ; Wed, 14 Nov 2007 08:46:38 +1200 12, 27 -- Received: from AKLEXB01.hort.net.nz ([10.16.1.15]) by aklexf01.hort.net.nz with Microsoft SMTPSVC(6.0.3790.3959); 12, 27 -- Wed, 14 Nov 2007 08:46:57 +1300 12, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 27 -- Content-class: urn:content-classes:message 12, 27 -- MIME-Version: 1.0 12, 27 -- Content-Type: text/plain; 12, 27 -- charset="us-ascii" 12, 27 -- Subject: RE: [Microscopy] TEM: Strange Sample Rod 12, 27 -- Date: Wed, 14 Nov 2007 08:46:56 +1300 12, 27 -- Message-ID: {D3BAD63C088F3C48AEB385E881359F8703B4C3CE-at-AKLEXB01.hort.net.nz} 12, 27 -- In-Reply-To: {200711131015.lADAFgdH023324-at-ns.microscopy.com} 12, 27 -- X-MS-Has-Attach: 12, 27 -- X-MS-TNEF-Correlator: 12, 27 -- Thread-Topic: [Microscopy] TEM: Strange Sample Rod 12, 27 -- Thread-Index: Acgl3ieNXj6DkrOqRCqg41Cdn1nocQATpY1w 12, 27 -- From: "Ian Hallett" {IHallett-at-hortresearch.co.nz} 12, 27 -- To: {john.mitchels-at-gmail.com} 12, 27 -- Cc: {microscopy-at-microscopy.com} 12, 27 -- X-OriginalArrivalTime: 13 Nov 2007 19:46:57.0383 (UTC) FILETIME=[F3173F70:01C8262D] 12, 27 -- Content-Transfer-Encoding: 8bit 12, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lADJl8CI013784 ==============================End of - Headers==============================
My wife and I were visiting friends and it was mentioned that they had an old microscope in their attic they were planning on giving to their 6-year old grandson. They wanted me to look at it for my opinion on its suitability as a gift for a young person.
They produced a fine wooden box, obviously very old. In it was a brass E. Leitz monocular microscope with the following information on an information card inside the door:
Mikroskop Nr. 27268 Vergrosserungen bei 160mm Tubuslange Wetzler den 29 VII 1893 E. Leitz
Everything was dusty but there was no evidence of water damage and everything worked smoothly. There was an engraved metal name plate on the box that said "Dr. A. Steere."
The numbers 1, 3, 5 and 3, 5, 7 were engraved on the eyepiece and objective lenses. As the data is obviously a magnification table, the lens numbers are not magnification power as is the modern practice. Specimen illuminations was via a mirror under the specimen stage.
Inside the box were several small drawers that contained slides, cover slips, small tools, and miscellaneous pill boxes and bottles that apparently were for specimen preparation. Nothing was labeled, the bottles were empty. One of the pillboxes contained a number of small cylindrical objects, about the size of pencil erasures, that I was smart enough not to touch. There was also a book with a 1940's copyright on using a microscope in English--clearly not related to the instrument.
I convinced our friends that the microscope dated from 1893 (not the 1940s as they thought) and it was hugely unsuitable for a 6-year old.
They don't want the microscope but asked: What to do with it? Is it valuable? A ballpark value range given the above description? Would a museum be interested in it? E-Bay?
We are open to suggestion that we can pass on to our friends.
Ron Anderson
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Focus - as you adjust the objective focus, you should see the lines move relative to the particle. There will also be changes in contrast.
Probably haven't got the explanation exactly right but my understanding is that you are seeing delocalisation arising from the spherical abberation of the objective lens. That is, because the diffracted beams contributing to the lattice image are following different, off-axis paths through the objective, the spherical aberation of the lens brings then to focus in a different plane to the undiffracted beam. Different diffracted beams follow different paths, so different sets of planes will have different relationships to the particle. As you change the objective lens, essentiially, you moving the image plane along the optic axis - the diffracted beams, making up the lattice images will therefore appear to move relative to the particle.
If you image the sample in an aberation corrected TEM with Cs~=0, then the lattice image will be exactly coincident with the particle.
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==============================Original Headers============================== 7, 15 -- From larry-at-cymru666.plus.com Tue Nov 13 14:10:36 2007 7, 15 -- Received: from fhw-relay07.plus.net (fhw-relay07.plus.net [212.159.14.215]) 7, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lADKAaLP027179 7, 15 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 13 Nov 2007 14:10:36 -0600 7, 15 -- Received: from [87.113.74.40] (helo=[192.168.1.2]) 7, 15 -- by fhw-relay07.plus.net with esmtp (Exim) id 1Is25j-0008MG-0W; Tue, 13 Nov 2007 20:10:35 +0000 7, 15 -- Mime-Version: 1.0 7, 15 -- Message-Id: {p06240800c35fb257a9e8-at-[192.168.1.2]} 7, 15 -- In-Reply-To: {200711131852.lADIqZ4p010953-at-ns.microscopy.com} 7, 15 -- References: {200711131852.lADIqZ4p010953-at-ns.microscopy.com} 7, 15 -- Date: Tue, 13 Nov 2007 20:06:59 +0000 7, 15 -- To: jmkrupp-at-ucsc.edu, Microscopy-at-MSA.Microscopy.Com 7, 15 -- From: Larry Stoter {larry-at-cymru666.plus.com} 7, 15 -- Subject: Re: [Microscopy] lattice fringes 7, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jlgilbert-at-ehs.unc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jlgilbert-at-ehs.unc.edu Name: James Gilbert
Organization: University of North Carolina at Chapel Hill
Title-Subject: [Filtered] EM-Refrigerated ethane
Question: A researcher on my campus has had to place a 4-ft cylinder of ultrapure ethane in a cold room to further remove oils from his experiments. If anyone has had to do this, how have you done it safely?
Thanks to everyone who replied to my alignment question. I checked the beam with the specimen holder and moveable apertures out of the the beam path and still had large beam shifts during focusing. At low mag it goes almost off the screen, the shifts direction and kind of circles around.
X-from your replies, I've concluded it's an alignment issue that our service engineer will have to deal with. At least I'm reasonably sure that it's not a routine fix I intend to take on myself at this point.
Thanks again to all.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 6, 23 -- From TindallR-at-missouri.edu Tue Nov 13 14:54:21 2007 6, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lADKsK7x030997 6, 23 -- for {microscopy-at-microscopy.com} ; Tue, 13 Nov 2007 14:54:20 -0600 6, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 6, 23 -- Tue, 13 Nov 2007 14:54:19 -0600 6, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 23 -- Content-class: urn:content-classes:message 6, 23 -- MIME-Version: 1.0 6, 23 -- Content-Type: text/plain; 6, 23 -- charset="us-ascii" 6, 23 -- Subject: TEM: Alignment Problem---Thanks 6, 23 -- Date: Tue, 13 Nov 2007 14:53:40 -0600 6, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4101C0BEEC-at-UM-XMAIL08.um.umsystem.edu} 6, 23 -- X-MS-Has-Attach: 6, 23 -- X-MS-TNEF-Correlator: 6, 23 -- Thread-Topic: TEM: Alignment Problem---Thanks 6, 23 -- thread-index: AcgmN0aAbAd0yCn9TIKOjx9IaN/jMA== 6, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 6, 23 -- To: {microscopy-at-microscopy.com} 6, 23 -- X-OriginalArrivalTime: 13 Nov 2007 20:54:19.0632 (UTC) FILETIME=[5C757F00:01C82637] 6, 23 -- Content-Transfer-Encoding: 8bit 6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lADKsK7x030997 ==============================End of - Headers==============================
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Ron -
The New York Microscopical Society has a properly maintained public microscope museum in their Montclair, NJ headquarters. They can probably provide value information & would be a good place for a donation - which could give your friend a nice tax deduction.
The 6-year old needs a 30x dissecting scope, which should cost $70. Comments - as you know - will be in the November Microscopy Today.
Caroline
-- Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 7, 18 -- From schooley-at-mcn.org Tue Nov 13 15:04:12 2007 7, 18 -- Received: from dns4.mcn.org (dns4.mcn.org [216.150.240.31]) 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lADL4BL1011751 7, 18 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 13 Nov 2007 15:04:12 -0600 7, 18 -- Received: from [66.81.74.10] (helo=[10.0.1.2]) 7, 18 -- by dns4.mcn.org with esmtpa (Exim 4.60) 7, 18 -- (envelope-from {schooley-at-mcn.org} ) 7, 18 -- id JRGQIW-000CKL-32; Tue, 13 Nov 2007 13:04:09 -0800 7, 18 -- Mime-Version: 1.0 7, 18 -- Message-Id: {a06200701c35fbed4bca3-at-[10.0.1.2]} 7, 18 -- In-Reply-To: {200711132016.lADKGcNF014508-at-ns.microscopy.com} 7, 18 -- References: {200711132016.lADKGcNF014508-at-ns.microscopy.com} 7, 18 -- Date: Tue, 13 Nov 2007 13:05:46 -0800 7, 18 -- To: randerson20-at-tampabay.rr.com 7, 18 -- From: Caroline Schooley {schooley-at-mcn.org} 7, 18 -- Subject: Re: [Microscopy] Antique Microscope 7, 18 -- Cc: Microscopy-at-MSA.Microscopy.Com, dkoleary-at-verizon.net 7, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I am trying to find a reasonably inexpensive way for students to practice using our ultramicrotome, without the potential for damaging the diamond knives. I have not had any luck making glass knives using tools we have available, and the knife cutters are quite expensive. Plus, the whole process of gluing the boat onto the knife, or using tape and nail polish, seems a bit cumbersome. I'm not so concerned with whether the sections are electron-thin at this point, only that we can develop the method.
I was hoping it would be possible to buy cheap steel knives that could fit into the knife holder for our RMC ultramicrotome. This would seem natural, since we already use razor blades to trim the blocks. We just need some way to mount the blades, but I can't locate any type of steel knife that is designed to fit in the ultramicrotome holder. The other possibilities I have come across are sapphire and tungsten carbide knives. The tungsten carbide knives at least come in a triangular shape, so we just need some tape and nail polish to make the boat. We can always switch to the diamond knife when we need ultra-thin sections.
I would be grateful for any suggestions someone may have. I thought there might be some type of boat available that can hold razor blades. Maybe the only option is to be more protective of the diamond knives. We are mainly cutting polymeric materials embedded in resin. Thanks.
-Phil ------------------------------------------ Phil Ahrenkiel, Assistant Professor Nanoscience and Nanoengineering Ph.D. Program South Dakota School of Mines and Technology 501 E. Saint Joseph St. Rapid City, South Dakota 57701 Office: EP 221 Phone: 605-394-5238, Fax: 605-394-2365 Email: Phil.Ahrenkiel-at-sdsmt.edu
==============================Original Headers============================== 6, 20 -- From Phil.Ahrenkiel-at-sdsmt.edu Tue Nov 13 17:16:38 2007 6, 20 -- Received: from owa.sdsmt.edu (owa.sdsmt.edu [151.159.3.7]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lADNGcDA026941 6, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Nov 2007 17:16:38 -0600 6, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 20 -- Content-class: urn:content-classes:message 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="us-ascii" 6, 20 -- Subject: alternative ultramicrotome knives 6, 20 -- Date: Tue, 13 Nov 2007 16:16:36 -0700 6, 20 -- Message-ID: {6FD01225FACD9C4AA3870724330536F407C648AD-at-sdsmt-ex01.SDSMT.LOCAL} 6, 20 -- X-MS-Has-Attach: 6, 20 -- X-MS-TNEF-Correlator: 6, 20 -- Thread-Topic: alternative ultramicrotome knives 6, 20 -- Thread-Index: AcgmSzzxcS+ymRc2Qrq8YRYr2wXloA== 6, 20 -- From: "Ahrenkiel, Scott (Phil) P." {Phil.Ahrenkiel-at-sdsmt.edu} 6, 20 -- To: {Microscopy-at-microscopy.com} 6, 20 -- Content-Transfer-Encoding: 8bit 6, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lADNGcDA026941 ==============================End of - Headers==============================
I understand your problem. To be honest, your best bet for students really is a glass knife. It does take some practice, but is tremendously cheaper than having someone destroy a several thousand dollar diamond knife. On the other hand, I have often wondered why some creative person does not sell "premade glass knives" with the trough attached. Perhaps someone will contact you in this regard. I believe this would be your BEST solution.
What sort of tools are you using to make the glass knives? If you are using standard glazier's tools (scoring wheel, glazier pliers), then I can understand your problem since it takes a lot of practice. Also, you should be using special glass strips designed for making ultramicrotomy knives rather than trying to break large pieces of plate glass. You might check out eBay, since I see items like glass knife makers and even ultramicrotomes selling very inexpensively ($200 and $500, respectively). Maybe some kind soul would donate a knife maker to you.
Alternatives: I don't know how much they cost, but tungsten carbide knives are not exactly throw away knives. Razor blades are OK for rough trimming but would quickly dull when cutting resins so as to make it inconvenient. Sapphire knives are not cheap and they are easily damaged (and are not resharpened).
Good luck and keep us posted on your progress.
JB
} I am trying to find a reasonably inexpensive way for students to } practice using our ultramicrotome, without the potential for damaging } the diamond knives. I have not had any luck making glass knives using } tools we have available, and the knife cutters are quite expensive. } Plus, the whole process of gluing the boat onto the knife, or using tape } and nail polish, seems a bit cumbersome. I'm not so concerned with } whether the sections are electron-thin at this point, only that we can } develop the method. } } I was hoping it would be possible to buy cheap steel knives that could } fit into the knife holder for our RMC ultramicrotome. This would seem } natural, since we already use razor blades to trim the blocks. We just } need some way to mount the blades, but I can't locate any type of steel } knife that is designed to fit in the ultramicrotome holder. The other } possibilities I have come across are sapphire and tungsten carbide } knives. The tungsten carbide knives at least come in a triangular shape, } so we just need some tape and nail polish to make the boat. We can } always switch to the diamond knife when we need ultra-thin sections. } } I would be grateful for any suggestions someone may have. I thought } there might be some type of boat available that can hold razor blades. } Maybe the only option is to be more protective of the diamond knives. We } are mainly cutting polymeric materials embedded in resin. Thanks. } } -Phil } ------------------------------------------ } Phil Ahrenkiel, Assistant Professor
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
I grew up on the old Keith Porter "Free Break" method of making glass knives. If you start with a square of glass 1/4 to 1/2" thick. and 4" square, you can make a small score (1/4") halfway along one edge, perpendicular to the edge, and then cause a break with curved glaziers pliers. It may be hard to find the curved pliers, but you might be able to get them from EF Fullham. If not, you can attach toothpicks to a standard set of glaziers pliers such that one side has a single toothpick in the center, and the other has two that flank the center. You can then squeeeeze very gently, and watch the crack progress across the glass (if you are lucky). This process can be continued until you have 1" "squares". A diagonal score, followed by more squeezing will often give you a good knife. If the whole tape/boat business is too much trouble, you can place a large bead of wax along the edge of the knife. (Make sure that the glass is clean first). Remarkably, this will hold sufficient water for sections to float off.
I can't send a diagram through the server, but I could probably put something together that I can send you directly. Let me know.
Joel
Date sent: Tue, 13 Nov 2007 17:16:48 -0600 To: jbs-at-temple.edu X-from: Phil.Ahrenkiel-at-sdsmt.edu Send reply to: Phil.Ahrenkiel-at-sdsmt.edu
Greetings Dale,
The edge flow legend is largely just that. Over the years, I found that one could prepare a batch of knives several weeks to months in advance and still use them successfully. I never said anything about it since it seemed to go against the grain and seemed to be a bit lazy (rather than efficient). Recently, Herb Hagler (perhaps he will chime in at some point) officially stated the following: "The old mythical tale that glass knives must be made fresh just is not true. If care is taken in the making of high-quality glass knives, they may be stored for many months to years and used repeatedly until they become unusable after 5 to 15 or more uses for thin sectioning." This quote is on page 70 of his chapter "Ultramicrotomy for Biological Electron Microscopy" in: Electron Microscopy Methods and Protocols (2nd Ed), Edited by John Kuo. Humana Press. ISBN 13: 978-1-58829-573-6.
In other words, the glass knives are treated like a diamond knife, in many ways, when cutting ultrathin sections. They are not so long lasting when cutting thicker sections, however.
Your observation about the loss of wetability of the knife edge is exactly what I have seen and the major reason why I was forced to make knives within a couple days of use. In fact, I could actually see a whitish film on the surface of the knife and assumed this was condensate of laboratory fumes and organics from the paraffin used to seal the trough -- or maybe even from the adhesives when tape was used to seal the trough. Your idea to use a plasma cleaner is really neat! Thanks for sharing your observations.
John Bozzola
} dale callaham wrote:
} Hi John, } } Regarding selling pre-made glass knives, I'm sure you are aware of } the (somewhat true) urban legends about glass being a liquid and the } edge flowing over time - I'm sure this isn't going to be an issue at } the level of training students being discussed, but it is going to } be hard for some people to turn loose of that one.... One thing I } have observed, may be related to our local indoor environment or } could be more general, but glass that I have broken more than a few } days before loses it's edge wetting properties. I've never seen it } mentioned anywhere. I have used our Harrick Plasma Cleaner for 10sec } on older knives (bare, or with tape or waxed-metal troughs already } attached) to make them hydrophilic again. } } Cheers, } } Dale Callaham } } bozzola-at-siu.edu wrote: } } } } Hello Phil, } } } } I understand your problem. To be honest, your best bet for students } } really is a glass knife. It does take some practice, but is } } tremendously cheaper than having someone destroy a several thousand } } dollar diamond knife. On the other hand, I have often wondered why } } some creative person does not sell "premade glass knives" with the } } trough attached. Perhaps someone will contact you in this regard. I } } believe this would be your BEST solution. } } } } What sort of tools are you using to make the glass knives? If you } } are using standard glazier's tools (scoring wheel, glazier pliers), } } then I can understand your problem since it takes a lot of } } practice. Also, you should be using special glass strips designed } } for making ultramicrotomy knives rather than trying to break large } } pieces of plate glass. You might check out eBay, since I see items } } like glass knife makers and even ultramicrotomes selling very } } inexpensively ($200 and $500, respectively). Maybe some kind soul } } would donate a knife maker to you. } } } } Alternatives: I don't know how much they cost, but tungsten carbide } } knives are not exactly throw away knives. Razor blades are OK for } } rough trimming but would quickly dull when cutting resins so as to } } make it inconvenient. Sapphire knives are not cheap and they are } } easily damaged (and are not resharpened). } } } } Good luck and keep us posted on your progress. } } } } JB } } } } } I am trying to find a reasonably inexpensive way for students to } } } practice using our ultramicrotome, without the potential for damaging } } } the diamond knives. I have not had any luck making glass knives using } } } tools we have available, and the knife cutters are quite expensive. } } } Plus, the whole process of gluing the boat onto the knife, or using tape } } } and nail polish, seems a bit cumbersome. I'm not so concerned with } } } whether the sections are electron-thin at this point, only that we can } } } develop the method. } } } } } } I was hoping it would be possible to buy cheap steel knives that could } } } fit into the knife holder for our RMC ultramicrotome. This would seem } } } natural, since we already use razor blades to trim the blocks. We just } } } need some way to mount the blades, but I can't locate any type of steel } } } knife that is designed to fit in the ultramicrotome holder. The other } } } possibilities I have come across are sapphire and tungsten carbide } } } knives. The tungsten carbide knives at least come in a triangular shape, } } } so we just need some tape and nail polish to make the boat. We can } } } always switch to the diamond knife when we need ultra-thin sections. } } } } } } I would be grateful for any suggestions someone may have. I thought } } } there might be some type of boat available that can hold razor blades. } } } Maybe the only option is to be more protective of the diamond knives. We } } } are mainly cutting polymeric materials embedded in resin. Thanks. } } } } } } -Phil } } } ------------------------------------------ } } } Phil Ahrenkiel, Assistant Professor
==============================Original Headers============================== 11, 20 -- From bozzola-at-siu.edu Tue Nov 13 19:31:14 2007 11, 20 -- Received: from cstmta4.siu.edu (cstmta4.siu.edu [131.230.1.4]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAE1VCMG032242 11, 20 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 13 Nov 2007 19:31:14 -0600 11, 20 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136]) 11, 20 -- by cstmta4.siu.edu (Switch-3.3.0/Switch-3.3.0) with ESMTP id lAE1VBTA026168 11, 20 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 13 Nov 2007 19:31:11 -0600 (CST) 11, 20 -- Mime-Version: 1.0 11, 20 -- Message-Id: {a06240806c35ff98848b5-at-[131.230.177.136]} 11, 20 -- In-Reply-To: {473A41D0.8010904-at-research.umass.edu} 11, 20 -- References: {200711132355.lADNtU20016455-at-ns.microscopy.com} 11, 20 -- {473A41D0.8010904-at-research.umass.edu} 11, 20 -- Date: Tue, 13 Nov 2007 19:31:09 -0600 11, 20 -- To: Microscopy-at-msa.microscopy.com 11, 20 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 11, 20 -- Subject: Re: [Microscopy] Re: alternative ultramicrotome knives 11, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 11, 20 -- X-Spam-Score: 0.00% 11, 20 -- X-MASF: 0.00% 11, 20 -- X-Whitelist: 0.00% ==============================End of - Headers==============================
With regard to your optimum under focus adjustment, one needs to rely upon the manufacturer to provide such a facility, not all do! In the JEOL, depending upon your magnification the instrument, when set up under wobbler focus, gave the wobbler/true focus and then stepped underfocus by a set amount when the focus control was released. If we did the job by eye we would probably set the ouf of a lower than JEOL set it, but it was a good start.
I train people to set wobbler focus and then to watch the focal change as they move underfocus until they see what is in their mind their own ouf. Take images in steps either side of this point to see "on a print" what people in the lab agree is the best micrograph - your ouf!
Plotting graphs relating to ouf for a particular material (organelle density) makes life a good deal easier if a novice is using the instrument - set wobbler focus and from the graph set the ouf for that organelle density - easy. Be aware that ouf changes with, kV, magnification, organelle density, section thickness
Hope this helps?
Steve Chapman Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
----- Original Message ----- X-from: {a.d.mckinnon-at-abdn.ac.uk} To: {protrain-at-emcourses.com} Sent: Tuesday, November 13, 2007 4:04 PM
Hi Randy
I have until now missed this topic but may I add data to help with the understanding?
Lenses have fields that overlap, we do not want them to do so but they do! This problem is most noticeable when the final condenser lens picks up a focal change due to the stronger objective field impinging on the condenser field as an offset.
In the past manufacturers have approached this problem in a number of ways. JEOL placed an equal and opposite deflection on their "balancing coils" so that we did not see the effect. Hitachi used a top hat objective pole piece to attempt to reduce the field overlap. Philips always balanced the illumination movement by adjusting the relationships of the upper and lower pole pieces. I do not know which route is taken with modern instruments but I am sure the problem is dialled out in some way.
My worry is why you have just noticed the problem, I would guess there may be another "problem" that has emphasised the condenser movement. I would ask if your current objective focal length (lens current) or condenser lens currents are normal, or do you have a malfunction in the objective area, lens current or deflection system. This would be typical of such a fault in that the lenses are not running at their correct values?
Let me know the cure as we may be able to help others that will suffer in the future.
Regards
Steve
Steve Chapman Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967 ----- Original Message ----- X-from: {TindallR-at-missouri.edu} To: {protrain-at-emcourses.com} Sent: Tuesday, November 13, 2007 8:55 PM
I was able to buy curved glazier's shears and a scriber at a store that sells glass and tools to artists who work with stained glass.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 3, 23 -- From donc-at-asmicro.com Tue Nov 13 21:26:10 2007 3, 23 -- Received: from smtp114.sbc.mail.re2.yahoo.com (smtp114.sbc.mail.re2.yahoo.com [68.142.229.91]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lAE3Q8g9025167 3, 23 -- for {microscopy-at-microscopy.com} ; Tue, 13 Nov 2007 21:26:09 -0600 3, 23 -- Received: (qmail 46681 invoked from network); 14 Nov 2007 03:26:08 -0000 3, 23 -- Received: from unknown (HELO asm15) (asmicro-at-sbcglobal.net-at-68.58.145.13 with login) 3, 23 -- by smtp114.sbc.mail.re2.yahoo.com with SMTP; 14 Nov 2007 03:26:07 -0000 3, 23 -- X-YMail-OSG: IbUq6iMVM1m16HP2IIMoX0soPs25abLFQKMBRmPf0YAGNbohikFdd1v7g58YFKGk1FwA6ZrKFsVKUpkfVQJV9n_SLXT.1qlWfCKfi3KxFAgyCKwsWH4- 3, 23 -- Message-ID: {001f01c8266e$06fb7520$0302a8c0-at-asm15} 3, 23 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 3, 23 -- To: "Microscopy List" {microscopy-at-microscopy.com} 3, 23 -- Subject: [Microscopy] Re: alternative ultramicrotome knives 3, 23 -- Date: Tue, 13 Nov 2007 22:12:36 -0500 3, 23 -- MIME-Version: 1.0 3, 23 -- Content-Type: text/plain; 3, 23 -- format=flowed; 3, 23 -- charset="iso-8859-1"; 3, 23 -- reply-type=original 3, 23 -- Content-Transfer-Encoding: 7bit 3, 23 -- X-Priority: 3 3, 23 -- X-MSMail-Priority: Normal 3, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 3, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
Dear colleagues, please kindly bring the following announcement to the attention of possible candidates: please note that there is no assignment of the number of fellowships to any department: if 5 physicists are the best candidates, they will get the positions. Also: the success rates for getting tenure have been very good so far and career advancement was often faster than the mandatory 5 years (see heading "Recent Fellows" on the webpage. So please encourage women scientists you know to apply. Thank you, best regards Petra Rudolf
Rosalind Franklin Fellowships for women in Arts and Sciences (tenure-track) Rijksuniversiteit Groningen, the Netherlands
To promote the participation of women in Liberal Arts and Natural Sciences the University of Groningen offers a prestigious fellowship programme, named after Rosalind Franklin, whose X-ray studies of DNA were crucial to solving its structure. Five fellowships are available in the Faculty of Mathematics and Natural Sciences. The fellowships will be awarded to outstanding women scientists from any of the disciplines mathematics, physics, astronomy, chemistry, biology, pharmacy, environmental studies, computing science and artificial intelligence. For further information see http://www.rug.nl/fwn/onderzoek/rff/index
APPLICANTS MUST HAVE: - a Ph.D and post-doctoral experience, preferably in different research institutions (Dutch applicants should have minimally 2 years post-doctoral experience outside the Netherlands). - publications in first rate international scientific journals - experience in supervising research projects - the ability to successfully compete for external research funding - affinity to teaching - evidence of international recognition We are looking for ambitious, creative women who aim for a successful independent career towards full professorship in a European top research university. Successful candidates will be expected to establish an independent, externally funded research program in collaboration with colleagues at our university and elsewhere. They will also be expected to participate in and contribute to the development of the teaching programme of the Faculty. RF Fellowships are funded with a generous startup package, worth around 200,000 euro.
The Rosalind Franklin fellowships follow the general tenure track career path for scientists in the Faculty of Mathematics and Natural Sciences. For a detailed description go to the following link: http://www.rug.nl/fwn/vacatures/rff/tenureTrack
APPLICANTS SHOULD SUBMIT: 1. a full curriculum vitae including a complete list of publications 2. a list of five selected "best papers" (no copies) 3. a 3-5 page statement of research accomplishments and future research goals 4. 3 letters of recommendation
To : Dr. L.J.A. van Putten Faculty Board office Faculty of Mathematics and Natural Sciences University of Groningen P.O. Box 407 9700 AK Groningen The Netherlands
THE DEADLINE is January 15, 2008
} } Prof. Dr. Petra Rudolf } Head of the Surfaces and Thin Films group } Zernike Institute of Advanced Materials } University of Groningen } Nijenborgh 4 } 9747 AG Groningen, the Netherlands } phone: +(31)50-363 4736 } e-mail: P.Rudolf-at-rug.nl } web page: http://www.surfacesthinfilms.fmns.rug.nl/ } } secretary: Sonja Groot } phone: +(31)50-363 4826 } fax: +(31)50-363 4879 } e-mail: s.e.a.groot-at-rug.nl / solidstate-at-rug.nl } } ------=_NextPart_000_0164_01C82699.D325A2D0 } Content-Type: text/html; } charset="iso-8859-1" } Content-Transfer-Encoding: quoted-printable } } |--!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN"--| } |--HTML--||--HEAD--| } |--META http-equiv=3DContent-Type content=3D"text/html; = } charset=3Diso-8859-1"--| } |--META content=3D"MSHTML 6.00.6000.16544" name=3DGENERATOR--| } |--STYLE--||--/STYLE--| } |--/HEAD--| } |--BODY bgColor=3D#ffffff--| } |--DIV--||--FONT face=3DArial size=3D2--|Dear } colleagues,|--/FONT--||--/DIV--| } |--DIV--||--FONT face=3DArial size=3D2--|please bring the following } announcement = } to the=20 } attention of possible candidates: please note that there is no = } assignment of the=20 } number of fellowships to any department: if 5 physicists are the } best=20 } candidates, they will get the positions. Also: the success rates for } = } getting=20 } tenure have been very good so far and career advancement was often = } faster than=20 } the mandatory 5 years (see heading "Recent Fellows" on the webpage. } So = } please=20 } encourage women scientists you know to apply.|--/FONT--||--/DIV--| } |--DIV--||--FONT face=3DArial size=3D2--|Thank } you,|--/FONT--||--/DIV--| } |--DIV--||--FONT face=3DArial size=3D2--|best } regards|--/FONT--||--/DIV--| } |--DIV--||--FONT face=3DArial size=3D2--|Petra } Rudolf|--/FONT--||--/DIV--| } |--DIV--||--FONT face=3DArial size=3D2--||--/FONT--| |--/DIV--| } |--DIV--||--FONT face=3DArial size=3D2--||--FONT face=3D"Times New } Roman" = } size=3D3--|Rosalind=20 } Franklin Fellowships for women in Arts and Sciences=20 } (tenure-track)|--BR--|Rijksuniversiteit Groningen, the = } Netherlands|--BR--||--BR--||--BR--|To=20 } promote the participation of women in Liberal Arts and Natural } Sciences = } the=20 } University of Groningen offers a prestigious fellowship programme, } named = } after=20 } Rosalind Franklin, whose X-ray studies of DNA were crucial to } solving = } its=20 } structure. Five fellowships are available in the Faculty of } Mathematics = } and=20 } Natural Sciences. The fellowships will be awarded to outstanding } women=20 } scientists from any of the disciplines mathematics, physics, } astronomy,=20 } chemistry, biology, pharmacy, environmental studies, computing } science = } and=20 } artificial intelligence. For further information see } |--/FONT--||--A=20 } href=3D"http://www.rug.nl/fwn/onderzoek/rff/index"--||--FONT } face=3D"Times = } New Roman"=20 } size=3D3--|http://www.rug.nl/fwn/onderzoek/rff/index|--/FONT--||--/A--||--BR--||--BR--||--FON= } T=20 } face=3D"Times New Roman" size=3D3--|APPLICANTS MUST HAVE:|--BR--|- a } Ph.D and=20 } post-doctoral experience, preferably in different research } institutions = } (Dutch=20 } applicants should have minimally 2 years post-doctoral } |--BR--|experience = } outside=20 } the Netherlands).|--BR--|- publications in first rate international } = } scientific=20 } journals|--BR--|- experience in supervising research } projects|--BR--|- the = } ability to=20 } successfully compete for external research funding|--BR--|- affinity } to=20 } teaching|--BR--|- evidence of international recognition|--BR--|We } are looking = } for=20 } ambitious, creative women who aim for a successful independent } career = } towards=20 } full professorship in a European top research |--BR--|university. } Successful = } } candidates will be expected to establish an independent, externally } = } funded=20 } research program in collaboration with |--BR--|colleagues at our } university = } and=20 } elsewhere. They will also be expected to participate in and } contribute = } to the=20 } development of the teaching programme |--BR--|of the Faculty. RF } Fellowships = } are=20 } funded with a generous startup package, worth around 200,000 = } euro.|--BR--||--BR--|The=20 } Rosalind Franklin fellowships follow the general tenure track career } = } path for=20 } scientists in the Faculty of Mathematics and Natural Sciences. For } |--BR--|a = } } detailed description go to the following link: |--/FONT--||--A=20 } href=3D"http://www.rug.nl/fwn/vacatures/rff/tenureTrack"--||--FONT=20 } face=3D"Times New Roman"=20 } size=3D3--|http://www.rug.nl/fwn/vacatures/rff/tenureTrack|--/FONT--||--/A--||--BR--||--B= } R--||--FONT=20 } face=3D"Times New Roman" size=3D3--|APPLICANTS SHOULD } SUBMIT:|--BR--|1. a full = } curriculum=20 } vitae including a complete list of publications|--BR--|2. a list of } five = } selected=20 } "best papers" (no copies)|--BR--|3. a 3-5 page statement of research } = } accomplishments=20 } and future research |--BR--|goals|--BR--|4. 3 letters of = } recommendation|--BR--||--BR--|To : Dr.=20 } L.J.A. van Putten|--BR--|Faculty Board office|--BR--|Faculty of } Mathematics and = } Natural=20 } Sciences|--BR--|University of Groningen|--BR--|P.O. Box } 407|--BR--|9700 AK = } Groningen|--BR--|The=20 } Netherlands|--BR--||--BR--|THE DEADLINE is January 15, = } 2008|--/FONT--||--BR--||--BR--||--/DIV--||--/FONT--| } |--DIV--||--FONT face=3DArial size=3D2--|Prof. Dr. Petra } Rudolf|--BR--|Head of the = } Surfaces and=20 } Thin Films group|--BR--|Zernike Institute of Advanced = } Materials|--BR--|University of=20 } Groningen|--BR--|Nijenborgh 4|--BR--|9747 AG Groningen, the = } Netherlands|--BR--|phone:=20 } +(31)50-363 4736|--BR--|e-mail: |--A=20 } href=3D"mailto:P.Rudolf-at-rug.nl"--|P.Rudolf-at-rug.nl|--/A--||--BR--|web } page: |--A=20 } href=3D"http://www.surfacesthinfilms.fmns.rug.nl/"--|http://www.surfacesthi= } nfilms.fmns.rug.nl/|--/A--||--/FONT--||--/DIV--| } |--DIV--| |--/DIV--| } |--DIV--||--FONT face=3DArial size=3D2--|secretary: Sonja } Groot|--BR--|phone: = } +(31)50-363=20 } 4826|--BR--|fax: +(31)50-363 4879|--BR--|e-mail: |--A=20 } href=3D"mailto:s.e.a.groot-at-rug.nl"--|s.e.a.groot-at-rug.nl|--/A--| / } |--A=20 } href=3D"mailto:solidstate-at-rug.nl"--|solidstate-at-rug.nl|--/A--||--BR--||--/FONT--||--/DIV--||--= } /BODY--||--/HTML--| } } ------=_NextPart_000_0164_01C82699.D325A2D0-- } } } } ==============================Original } Headers============================== } 22, 22 -- From p.rudolf-at-rug.nl Wed Nov 14 01:39:04 2007 } 22, 22 -- Received: from smtp1.rug.nl (smtp1.rug.nl [129.125.50.11]) } 22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id lAE7d2kK026258 } 22, 22 -- for |--microscopy-at-microscopy.com--|; Wed, 14 Nov 2007 } 01:39:03 -0600 } 22, 22 -- Received: from smtp1.rug.nl ([129.125.50.11]) } 22, 22 -- by smtp1.rug.nl (SMSSMTP 4.1.0.19) with SMTP id } M2007111408390107978 } 22, 22 -- for |--microscopy-at-microscopy.com--|; Wed, 14 Nov 2007 } 08:39:01 +0100 } 22, 22 -- Received: from your2b9e04e703 (pcvsf56.phys.rug.nl } [129.125.13.237]) } 22, 22 -- by smtp1.rug.nl (8.12.11.20060308/8.12.11) with SMTP id } lAE7csWu009933 } 22, 22 -- for |--Microscopy-at-Microscopy.Com--|; Wed, 14 Nov 2007 } 08:39:01 +0100 (MET) } 22, 22 -- Message-ID: } |--016701c82691$743ac0a0$ed0d7d81-at-your2b9e04e703--| } 22, 22 -- From: "Petra Rudolf" |--p.rudolf-at-rug.nl--| } 22, 22 -- To: |--Microscopy-at-microscopy.com--| } 22, 22 -- Subject: Tenure track Faculty positions in Groningen (NL) } 22, 22 -- Date: Wed, 14 Nov 2007 08:39:09 +0100 } 22, 22 -- MIME-Version: 1.0 } 22, 22 -- Content-Type: multipart/alternative; } 22, 22 -- boundary="----=_NextPart_000_0164_01C82699.D325A2D0" } 22, 22 -- X-Priority: 3 } 22, 22 -- X-MSMail-Priority: Normal } 22, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 } 22, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } ==============================End of - } Headers==============================
==============================Original Headers============================== 6, 24 -- From P.Rudolf-at-rug.nl Wed Nov 14 01:49:22 2007 6, 24 -- Received: from smtp1.rug.nl (smtp1.rug.nl [129.125.50.11]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lAE7nLXY026465 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 14 Nov 2007 01:49:22 -0600 6, 24 -- Received: from smtp1.rug.nl ([129.125.50.11]) 6, 24 -- by smtp1.rug.nl (SMSSMTP 4.1.0.19) with SMTP id M2007111408492108380 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 14 Nov 2007 08:49:21 +0100 6, 24 -- Received: from mail3.rug.nl (mail3.rug.nl [129.125.50.14]) 6, 24 -- by smtp1.rug.nl (8.12.11.20060308/8.12.11) with ESMTP id lAE7nL30011722 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 14 Nov 2007 08:49:21 +0100 (MET) 6, 24 -- Received: from [129.125.13.237] (account p225608-at-rug.nl) 6, 24 -- by mail3.rug.nl (CommuniGate Pro WEBUSER 5.1.12) 6, 24 -- with HTTP id 58859416 for microscopy-at-microscopy.com; Wed, 14 Nov 2007 08:49:21 +0100 6, 24 -- From: "P.Rudolf" {P.Rudolf-at-rug.nl} 6, 24 -- Subject: Tenure track Faculty positions in Groningen (NL) 6, 24 -- To: microscopy-at-microscopy.com 6, 24 -- X-Mailer: CommuniGate Pro WebUser v5.1.12 6, 24 -- Date: Wed, 14 Nov 2007 09:49:21 +0200 6, 24 -- Message-ID: {web-58859416-at-mail3.rug.nl} 6, 24 -- In-Reply-To: {200711140739.lAE7d42p026261-at-ns.microscopy.com} 6, 24 -- References: {200711140739.lAE7d42p026261-at-ns.microscopy.com} 6, 24 -- MIME-Version: 1.0 6, 24 -- Content-Type: text/plain;charset=utf-8;format="flowed" 6, 24 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I think you would be best sticking with glass knives if possible because they are cheap and all of your students can have a go.
The next thing is how best to make them. Well pliers are Ok but a knife making machine would be an awful lot better simply because the quality is much easier to guarantee.
GLASS KNIFE MAKING I assume cost will come into the equation, so a couple of alternatives occur to me: 1. try to buy or obtain a used or re-conditioned glass knife maker from another user or e.m spares supplier. 2. If nothing is available find out who has a knife maker and either try to arrange for them to supply you with glass knives at a near cost price or see if you could go over to their lab if nearby and make a lot up. This may mean storing a lot of pre-made glass knives safely or having them posted/freighted to you. It is possible to buy manufactured storage boxes or make your own with a little ingenuity and some appropriate containers and sticky tape. 3. As suggested elsewhere it is important to use the right glass. It should be float glass and most e.m. suppliers should be able to sell you some. We buy 30 strips of ultramicrotome glass (406mm x 25.4mm x 6.0mm) for a few tens of UK pounds and that will make up to ~1,000 knives.
WATER BATH MAKING When making water baths the easiest way is to use pre-made plastic or metal troughs which seal well with wax. Years ago I bought as many LKB Trufs (pre-made disposable plastic troughs) as I could afford and still have a reasonable stock. If this is difficult or expensive then use adhesive tape but experiment with different types. Some electrical plastic insulation tapes are good, as well as some of the aluminium tapes but one of my favourites was water-proof elastoplast tape on a roll because it was clean and the adhesive didn't dissolve too much in water. I still preferred to seal the outside of the tape with wax as well.
A hot-plate or a special wax hotplate (eg LKB 2208 multiplate) are invaluable for keeping the wax ready to use and I normally apply the wax with a thin spatula. I've always used dental wax for sealing water baths but I'm sure that paraffin wax will be just as good.
I hope this helps.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: Phil.Ahrenkiel-at-sdsmt.edu
I totally agree with you, a good knife maker and (good) glass is the way to go. Consistent good knives at a reasonable price as long as one is following the instructions for the knife maker! AND the handling of the knife! (As always: "It's the fool behind the tool") If anyone is looking for a reconditioned knife maker or other equipment, please email me off list. Regards, Markus F. Meyenhofer Microscopy Labs Box 338 61 West Street Red Bank, NJ 07701 732 747 6228 fax 732 758 9142 micro-at-superlink.net
----- Original Message ----- X-from: {malcolm.haswell-at-sunderland.ac.uk} To: {micro-at-superlink.net} Sent: Wednesday, November 14, 2007 7:03 AM
I totally agree with you, a good knife maker and (good) glass is the way to go. Consistent good knives at a reasonable price as long as one is following the instructions for the knife maker! AND the handling of the knife! (As always: "It's the fool behind the tool") If anyone is looking for a reconditioned knife maker or other equipment, please email me off list. Regards, Markus F. Meyenhofer Microscopy Labs Box 338 61 West Street Red Bank, NJ 07701 732 747 6228 fax 732 758 9142 micro-at-superlink.net
----- Original Message ----- X-from: {malcolm.haswell-at-sunderland.ac.uk} To: {micro-at-superlink.net} Sent: Wednesday, November 14, 2007 7:03 AM
I totally agree with you, a good knife maker and (good) glass is the way to go. Consistent good knives at a reasonable price as long as one is following the instructions for the knife maker! AND the handling of the knife! (As always: "It's the fool behind the tool") If anyone is looking for a reconditioned knife maker or other equipment, please email me off list. Regards, Markus F. Meyenhofer Microscopy Labs Box 338 61 West Street Red Bank, NJ 07701 732 747 6228 fax 732 758 9142 micro-at-superlink.net
----- Original Message ----- X-from: {malcolm.haswell-at-sunderland.ac.uk} To: {micro-at-superlink.net} Sent: Wednesday, November 14, 2007 7:03 AM
} Phil,
I just checked eBay and there is an LKB Knifemaker in excellent condition. Starting price is $595 and buy it now price is $695. No bids and closes in less than an hour. You might contact them afterwards and negotiate "a deal."
The item number is: 330185939588
Good luck.
John
} John- } } I did buy the glass strips from Ted Pella. Then I bought a glass } breaking tool at Hobby Lobby, but it is not durable enough to break the } strips. So I tried to make a contraption for breaking the knives using a } vise, but I can't get a clean break. The best one I got is when I broke } the glass with a hammer, but I can't reproduce that. } } A few people have offered to cut some glass knives for us, so that ought } to get us started. Then I will started looking into a second-hand knife } maker. } } Thanks for your input. } } -Phil } ------------------------------------------ } Phil Ahrenkiel, Assistant Professor } Nanoscience and Nanoengineering Ph.D. Program } South Dakota School of Mines and Technology } 501 E. Saint Joseph St. } Rapid City, South Dakota 57701 } Office: EP 221 } Phone: 605-394-5238, Fax: 605-394-2365 } Email: Phil.Ahrenkiel-at-sdsmt.edu } } } -----Original Message----- } From: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] } Sent: Tuesday, November 13, 2007 4:55 PM } To: Ahrenkiel, Scott (Phil) P. } Subject: [Microscopy] Re: alternative ultramicrotome knives } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
==============================Original Headers============================== 7, 22 -- From bozzola-at-siu.edu Wed Nov 14 14:20:06 2007 7, 22 -- Received: from cstmta3.siu.edu (cstmta3.siu.edu [131.230.1.3]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAEKK609006035 7, 22 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 14 Nov 2007 14:20:06 -0600 7, 22 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136]) 7, 22 -- by cstmta3.siu.edu (Switch-3.3.0/Switch-3.3.0) with ESMTP id lAEKK4jT013898; 7, 22 -- Wed, 14 Nov 2007 14:20:05 -0600 (CST) 7, 22 -- Mime-Version: 1.0 7, 22 -- Message-Id: {a06240808c3610811d1bb-at-[131.230.177.136]} 7, 22 -- In-Reply-To: 7, 22 -- {6FD01225FACD9C4AA3870724330536F407C64BE5-at-sdsmt-ex01.SDSMT.LOCAL} 7, 22 -- References: {200711132355.lADNt8ho015985-at-ns.microscopy.com} 7, 22 -- {6FD01225FACD9C4AA3870724330536F407C64BE5-at-sdsmt-ex01.SDSMT.LOCAL} 7, 22 -- Date: Wed, 14 Nov 2007 14:20:03 -0600 7, 22 -- To: Microscopy-at-msa.microscopy.com 7, 22 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 7, 22 -- Subject: RE: [Microscopy] Re: alternative ultramicrotome knives 7, 22 -- Cc: Phil.Ahrenkiel-at-sdsmt.edu 7, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 7, 22 -- X-Spam-Score: 0.00% 7, 22 -- X-MASF: 0.00% 7, 22 -- X-Whitelist: 0.00% ==============================End of - Headers==============================
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Dear Group: to date, some of these tools have extended their function into other areas of study such as biology. Is there anyone out there in our group that are using these for a particular biological application? Any comments will be greatly appreciated. Thanks so much. Barbara
==============================Original Headers============================== 2, 20 -- From maloneyb-at-fiu.edu Wed Nov 14 15:15:44 2007 2, 20 -- Received: from fmailhost05.isp.att.net (fmailhost05.isp.att.net [204.127.217.105]) 2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAELFiRJ031357 2, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Nov 2007 15:15:44 -0600 2, 20 -- Received: from [192.168.1.2] (adsl-074-166-189-137.sip.mia.bellsouth.net[74.166.189.137]) 2, 20 -- by bellsouth.net (frfwmhc05) with ESMTP 2, 20 -- id {20071114211543H0500f98pke} ; Wed, 14 Nov 2007 21:15:43 +0000 2, 20 -- X-Originating-IP: [74.166.189.137] 2, 20 -- Message-ID: {473B6565.1030304-at-fiu.edu} 2, 20 -- Disposition-Notification-To: barbara maloney {maloneyb-at-fiu.edu} 2, 20 -- Date: Wed, 14 Nov 2007 16:15:17 -0500 2, 20 -- From: barbara maloney {maloneyb-at-fiu.edu} 2, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 2, 20 -- X-Accept-Language: en-us, en 2, 20 -- MIME-Version: 1.0 2, 20 -- To: Microscopy-at-microscopy.com 2, 20 -- Subject: EBSD, EDS, WDS AND EPMA for biological applications 2, 20 -- X-Priority: 2 (High) 2, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 2, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi All - Here are the entire responses to my post.
Jessica Cervantes Bend Research, Inc Bend, OR
----- Have you looked at?
Silvander, M., G. Karlsson, et al. (1996). "Vesicle Solubilization by Alkyl Sulfate Surfactants: A Cryo-TEM Study of the Vesicle to Micelle Transition." Journal of Colloid and Interface Science 179: 104-113.
Stephen Murray Transmission Electron Microscopy Paterson Institute for Cancer Research University of Manchester Wilmslow Road Withington Manchester M20 4BX
----- Contact Brigitte Sternberg - Brigitte P Sternberg {brigittnanoanalytical-at-yahoo.com} . She has done a great deal of cryo work in this area. I had a chance to learn more about it at the recent SPIE meeting in San Diego and included that info in an upcoming article in America Lab (November, most likely): "Focus on Microscopy" column which can be found under "ARTICLES" at www.iscpubs.com (Jessica's Note: I could not find this article). Briggitte's website is http://nanoanalytical.netfirms.com
Hope this is helpful.
Barbara Foster, President
We've moved! Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 E: bfoster-at-mme1.com W: www.MicroscopyEducation.com
----- I think that your vitreous ice cryoTEM approach is the best choice to image micelles. Several years ago we were studying gallstone formation and attempted to visualize by cryoTEM the earliest stages of cholesterol nucleation using a supersaturated model bile composed of cholesterol, lecithin, and taurocholate in an aqueous solution with 0.15M NaCl. We were able to see various types of unilamellar and multilamellar vesicles in a background of micelles. Our homemade holey carbon films were relatively thick because we were trying to capture particles expected to be much larger than micelles. You might want to consider very thin carbon layers. I think we were single side blotting to concentrate the particles; there may be some trial and error in working out the blotting times You can take a look at the micrographs in the paper in Biophysical Journal Vol. 76, pp.1436-1451. There are more technical details and possibly some useful references. Good luck, Don
Donald Gantz Research Histologist/Electron Microscopist Dept. Physiology and Biophysics Center for Advanced Biomedical Research Boston University School of Medicine 715 Albany Street Boston, MA 02118 email: gantz-at-bu.edu
----- I have done a good deal of work with surfactants and I built a rather involved "Deep" Plunge freezer so I could do Freeze Fracture on them. FF is not so easy to get the hang of and with out a very well controlled FF system understanding what you are looking at can be daunting. Do you have a Cryo stage SEM available? I might be able to help you if you want to call and talk. Richard P. Gursky Sr. Applications Engineer FEI Company 5350 NE Dawson Creek Drive Hillsboro, OR 97124 Please Note New Email Address: Richard.Gursky-at-FEI.com
----- I don't know what your experience is with cryoEM and associated techniques, so most of what I have to say may be irrelevant. The problem you have posed (visualization of micelles) has been addressed in the biological cryoEM field on numerous occasions. Off the top of my head, I would suggest looking for work by Y. Talmon, perhaps starting as early as the mid- to late 1980's. For example, I just found (cut and pasted from a PubMed search, so I apologize for the colors and such):
The mechanism of lamellar-to-inverted hexagonal phase transitions: a study using temperature-jump cryo-electron microscopy.
This has some nice pictures of the sorts of images to expect, and gives a number of details about sample prep, imaging etc. There should be additional papers from this group, and this should lead you into the proper literature.
I also know that I reviewed a paper a year or two ago that was intended for the Journal of Physical Chemistry. It had images from a block copolymer study, but as I remember, it didn't have much detail for the cryoEM methods. However, it might get you into more recent literature. The paper I reviewed was not published in the form I saw it and I unfortunately can't remember the author's name - it was a single author and something Eastern European, but I realize that doesn't help very much. However, block copolymers and cryoEM might get you someplace...
Another place to check in the literature is Fred Sigworth (2001) Journal of Structural Biology 133, 119-131. That paper describes a method for computationally "erasing" the signal in an image due to a spherical lipid vesicle, but also contains some images of micelles and details about sample prep and imaging conditions.
This quick look into the literature should give you an idea of what to expect, and some details for dealing with sample prep and imaging conditions.
As to very general comments: These images will be very low contrast and must be collected using standard biological low dose techniques (limiting the total exposure of the area where the image is recorded to around 10 electrons per square Angstrom). That is _very_ low if you come out of the materials science community. Also, such images will need to be recorded at relatively low magnifications (around 50,000 on the film or CCD) - that's the only way to keep the dose low but the signal to noise high enough to see anything.
Another very critical factor will be the concentration of surfactant/micelles. Most surfactants have a very low critical micelle concentration (CMC) which means that they can be very dilute while still maintaining the structure of micelles. The sample needs to be dilute enough to have isolated micelles in an empty aqueous field, but not so dilute that you don't see anything. That can be a fine balance, and can generally only be empirically determined. The papers I mentioned above may give you some sort of idea of the concentrations used, but that information may only be marginally helpful for your particular problem.
Your note indicated that you couldn't see anything in the images you collected, but it's not clear what "nothing" means. The sample could have been too thick to get the beam through, too thick to get a good image (too much multiple scattering, for example), too concentrated to see individual micelles, too dilute (nice fields of ice, but nothing in them), no ice at all (beam damage eliminating all the ice, or "dry" regions of the grid), etc.
David Gene Morgan Chemical Engineering and Materials Science University of California at Davis
----- I've done artificial membranes/micelles for TEM - they are a pain. My standard prep is to do an osmium vapor fixation - you get contrast but don't change the buffer at all. Then the samples (if mostly lipid) can be embedded in agar & procesed as blocks of pseudo-tissue.
I just re-read your post - I've only tried to look at them just mounted on a grid (not embedded & sectioned) once, when we were having trouble imaging anything - so I'm not sure how they'll do if you do that. I just dried them down that time...we're not set up for vitrified samples. As it turned out, the lab making the micelles had changed lipids & the things weren't holding together at all - by their regular tests or by mine. I don't see why it wouldn't be worth at least a try with the cryo after osmium vapor.
Tamara Howard
----- Check out this feature http://nano.cancer.gov/news_center/monthly_feature_2005_jul.asp
Apparently also you can negative stain with PTA. Of course this introduces the staining artifact that cryo-em avoids. Maybe you can use it as a screen to make sure you have micelle's, ie quick sample check before doing the cryo. Good Luck
___________________
==============================Original Headers============================== 38, 14 -- From cervantes-at-bendres.com Wed Nov 14 15:27:39 2007 38, 14 -- Received: from mail.bendres.com (mail.bendres.com [216.228.161.112] (may be forged)) 38, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAELRdpk011180 38, 14 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Nov 2007 15:27:39 -0600 38, 14 -- MIME-Version: 1.0 38, 14 -- Content-Type: text/plain; 38, 14 -- charset="us-ascii" 38, 14 -- Subject: TEM Help with Imaging of Micelle Solutions - LONG recap 38, 14 -- Date: Wed, 14 Nov 2007 13:27:43 -0800 38, 14 -- Message-ID: {8943D65F9AD70E4488AD6DE09F15088768C870-at-BRIEX04A} 38, 14 -- From: "Cervantes, Jessica" {cervantes-at-bendres.com} 38, 14 -- To: {Microscopy-at-microscopy.com} 38, 14 -- Content-Transfer-Encoding: 8bit 38, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lAELRdpk011180 ==============================End of - Headers==============================
Well, I really opened a can of worms here. Some researchers asked me to make suggestions for a HRTEM to get them started with materials characterization. Thanks to the replies from this list, I am now the resident expert on this topic.
Now they want ideas for what kind of instrumentation to include in a materials characterization lab. This is at the total brainstorming/wish list level at this time, but you all are the experts.
So far some things like FETEM with EDS and EELS have been mentioned. Some have hinted at FIB/SEM, others mention XPS and XRD.
What should really go into a lab like this? What about specimen prep things? The researchers are several chemistry types looking at nanoparticles and some electrical engineers who look at thin films and some other things that I don't yet understand.
At this point, there is no budget and maybe no brains, but just a dream. We are pretty much starting from scratch, so let your imagination run wild. Also, how realistic would it be to include cryoEM as used by single particle reconstruction investigators in a lab like this or would they be better served by their own dedicated facility?
Does anyone know of a lab that might be used as a model? Maybe a web page that describes a successful operation. Sorry for all the questions, maybe you can just hit the high points that are most interesting to you.
Hoping someone comes up with a 'Marshall Plan' to rebuild scientific instrumentation soon.
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. Visit http://www.aidslifecycle.org for more information about the ride.
==============================Original Headers============================== 11, 20 -- From jmkrupp-at-ucsc.edu Wed Nov 14 17:46:36 2007 11, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAENkZ8a028391 11, 20 -- for {microscopy-at-microscopy.com} ; Wed, 14 Nov 2007 17:46:35 -0600 11, 20 -- Received: from [128.114.125.117] (HELO ucsc.edu) 11, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 11, 20 -- with ESMTPS id 26041052 for microscopy-at-microscopy.com; Wed, 14 Nov 2007 15:46:35 -0800 11, 20 -- Received: by email-prod-fe-1.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 11, 20 -- with PIPE id 20694291; Wed, 14 Nov 2007 15:46:35 -0800 11, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 11, 20 -- Received: from [128.114.25.127] (account jmkrupp-at-ucsc.edu HELO [128.114.25.127]) 11, 20 -- by email-prod-fe-1.ucsc.edu (CommuniGate Pro SMTP 5.1.11) 11, 20 -- with ESMTPA id 20694283 for microscopy-at-microscopy.com; Wed, 14 Nov 2007 15:46:34 -0800 11, 20 -- Mime-Version: 1.0 11, 20 -- Message-Id: {p06230903c3613486fbed-at-[128.114.25.127]} 11, 20 -- Date: Wed, 14 Nov 2007 15:46:32 -0800 11, 20 -- To: microscopy-at-microscopy.com 11, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 11, 20 -- Subject: Material characterization ideas 11, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
What kind or type of characterization do they want/need?
Thin films (how thin?) and CNTs and MWCNTs are quite different. What type of films? EDS is good to a point but WDS might work for some specimens. It seems you would need a mix of techniques to widely cover the base of specimens. HRSEM, EDS, STEM and EBSD ought to be good candidates for a SEM-based tool. Then look at options for HRTEM.
Apart from this, based on the specimens, they may have a whole different set of specimen prep issues, tools and costs.
These days, I think you would get more data from EBSD than XRD.
gary g.
At 03:48 PM 11/14/2007, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 8, 20 -- From gary-at-gaugler.com Wed Nov 14 18:05:02 2007 8, 20 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lAF051Hd008423 8, 20 -- for {microscopy-at-microscopy.com} ; Wed, 14 Nov 2007 18:05:01 -0600 8, 20 -- Message-Id: {200711150005.lAF051Hd008423-at-ns.microscopy.com} 8, 20 -- Received: (qmail 20237 invoked from network); 14 Nov 2007 16:04:58 -0800 8, 20 -- Received: by simscan 1.1.0 ppid: 20225, pid: 20233, t: 0.1833s 8, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 8, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 8, 20 -- by qsmtp2 with SMTP; 14 Nov 2007 16:04:58 -0800 8, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 20 -- Date: Wed, 14 Nov 2007 16:04:57 -0800 8, 20 -- To: jmkrupp-at-ucsc.edu 8, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 8, 20 -- Subject: Re: [Microscopy] Material characterization ideas 8, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 8, 20 -- In-Reply-To: {200711142348.lAENmuWZ031133-at-ns.microscopy.com} 8, 20 -- References: {200711142348.lAENmuWZ031133-at-ns.microscopy.com} 8, 20 -- Mime-Version: 1.0 8, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-30DE1B54 ==============================End of - Headers==============================
Lots of universities have facilities broadly similar to what you're considering, and they vary hugely in purpose and scope. The web page for the facility at UW Madison is msc.engr.wisc.edu. A list of facilities and a thorough discussion of the problems and opportunities they face may be found in the recent National Academies report "Midsize Facilities: Infrastructure for Materials Research", at www.nap.edu/catalog.php?record_id=11336.
Generally these facilities exist to house instruments that are too expensive for a single group to purchase or maintain (TEM, FESEM/FIB), or instruments that many groups want to use for only a fraction of the time available (XRD, XPS). They also usually provide in-house instrument maintenance and user training.
I think the best instruments for these facilities have one or two dedicated, expert users to act as champions, and a wide base of small users to fill up the time. I've never planned a new facility, but I suggest a survey of what your prospective users would be willing to champion, and what they want to use.
Good luck!
Best wishes, Paul Voyles
Paul Voyles Materials Science and Engineering University of Wisconsin, Madison 1509 University Ave, Rm 223 Madison, WI 53706-1595 voice: (608) 265-6740 fax: (608) 262-8353 voyles-at-engr.wisc.edu http://tem.msae.wisc.edu
jmkrupp-at-ucsc.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi: } } Well, I really opened a can of worms here. Some researchers asked me } to make suggestions for a HRTEM to get them started with materials } characterization. Thanks to the replies from this list, I am now the } resident expert on this topic. } } Now they want ideas for what kind of instrumentation to include in a } materials characterization lab. This is at the total } brainstorming/wish list level at this time, but you all are the } experts. } } So far some things like FETEM with EDS and EELS have been mentioned. } Some have hinted at FIB/SEM, others mention XPS and XRD. } } What should really go into a lab like this? What about specimen prep } things? The researchers are several chemistry types looking at } nanoparticles and some electrical engineers who look at thin films } and some other things that I don't yet understand. } } At this point, there is no budget and maybe no brains, but just a } dream. We are pretty much starting from scratch, so let your } imagination run wild. Also, how realistic would it be to include } cryoEM as used by single particle reconstruction investigators in a } lab like this or would they be better served by their own dedicated } facility? } } Does anyone know of a lab that might be used as a model? Maybe a web } page that describes a successful operation. Sorry for all the } questions, maybe you can just hit the high points that are most } interesting to you. } } Hoping someone comes up with a 'Marshall Plan' to rebuild scientific } instrumentation soon. } } Jon
==============================Original Headers============================== 11, 23 -- From voyles-at-engr.wisc.edu Wed Nov 14 18:58:33 2007 11, 23 -- Received: from mail.cae.wisc.edu (mail.cae.wisc.edu [144.92.13.31]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAF0wXRD021792 11, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Nov 2007 18:58:33 -0600 11, 23 -- Received: from smtpauth.cae.wisc.edu (smtpauth.cae.wisc.edu [144.92.13.83]) 11, 23 -- by mail.cae.wisc.edu (8.13.8+Sun/8.13.3) with ESMTP id lAF0wXjP005767 11, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Nov 2007 18:58:33 -0600 (CST) 11, 23 -- Received: from [192.168.1.129] (adsl-71-150-250-237.dsl.mdsnwi.sbcglobal.net [71.150.250.237]) 11, 23 -- (authenticated bits=0) 11, 23 -- by smtpauth.cae.wisc.edu (8.13.8/8.13.8/Debian-3) with ESMTP id lAF0wSnd017550 11, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 11, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Nov 2007 18:58:33 -0600 11, 23 -- Message-ID: {473B99AF.1030302-at-engr.wisc.edu} 11, 23 -- Date: Wed, 14 Nov 2007 18:58:23 -0600 11, 23 -- From: Paul Voyles {voyles-at-engr.wisc.edu} 11, 23 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 11, 23 -- MIME-Version: 1.0 11, 23 -- To: Microscopy-at-microscopy.com 11, 23 -- Subject: Re: [Microscopy] Material characterization ideas 11, 23 -- References: {200711142348.lAENmTnG030318-at-ns.microscopy.com} 11, 23 -- In-Reply-To: {200711142348.lAENmTnG030318-at-ns.microscopy.com} 11, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We all come with our own filters to any discussion and Biologists and Materials people insist on their own vocabularies and seldom try to learn each other's. I firmly believe that a lab facility can be "integrated" and "work" for both sides of the street. It would take someone to be "in charge" that can listen to and speak with both. Similar to the job done by a parent of two very different children, cherish and promote the best aspects and help work through the "hurdles".
Obviously I am someone with strong opinions. I would be glad to offer concrete suggestions offline.
My undergraduate degree is Chemistry, graduate degrees are Materials Science. I have academic, industrial and government lab TEM experience looking at catalysts, wafers, ICs, thin films, nano- structures, and now biological structures. HREM, EDS, EELS, STEM, FETEM (both in-column and post column).
Best regards, Roseann
Roseann Csencsits, PhD TEM Facility Manager Donner Lab Lawrence Berkeley Lab 1 Cyclotron Road Berkeley CA 94720 510-486-4548
On Nov 14, 2007, at 3:52 PM, jmkrupp-at-ucsc.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi: } } Well, I really opened a can of worms here. Some researchers asked me } to make suggestions for a HRTEM to get them started with materials } characterization. Thanks to the replies from this list, I am now the } resident expert on this topic. } } Now they want ideas for what kind of instrumentation to include in a } materials characterization lab. This is at the total } brainstorming/wish list level at this time, but you all are the } experts. } } So far some things like FETEM with EDS and EELS have been mentioned. } Some have hinted at FIB/SEM, others mention XPS and XRD. } } What should really go into a lab like this? What about specimen prep } things? The researchers are several chemistry types looking at } nanoparticles and some electrical engineers who look at thin films } and some other things that I don't yet understand. } } At this point, there is no budget and maybe no brains, but just a } dream. We are pretty much starting from scratch, so let your } imagination run wild. Also, how realistic would it be to include } cryoEM as used by single particle reconstruction investigators in a } lab like this or would they be better served by their own dedicated } facility? } } Does anyone know of a lab that might be used as a model? Maybe a web } page that describes a successful operation. Sorry for all the } questions, maybe you can just hit the high points that are most } interesting to you. } } Hoping someone comes up with a 'Marshall Plan' to rebuild scientific } instrumentation soon. } } Jon } -- } } Jonathan Krupp } Microscopy & Imaging Lab } C230 Earth & Marine Science } University of California } Santa Cruz, CA 95064 } (831) 459-2477 } jmkrupp-at-ucsc.edu } } I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money } for the San Francisco AIDS Foundation. Visit } http://www.aidslifecycle.org for more information about the ride.
==============================Original Headers============================== 12, 26 -- From RCsencsits-at-lbl.gov Thu Nov 15 14:25:59 2007 12, 26 -- Received: from ironport2.lbl.gov (ironport2.lbl.gov [128.3.41.14]) 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAFKPw7L024349 12, 26 -- for {microscopy-at-microscopy.com} ; Thu, 15 Nov 2007 14:25:58 -0600 12, 26 -- X-Ironport-SBRS: 3.5 12, 26 -- X-Brightmail-Tracker: AAAAAA== 12, 26 -- X-BrightmailFiltered: true 12, 26 -- X-IronPort-AV: E=Sophos;i="4.21,421,1188802800"; 12, 26 -- d="scan'208";a="47068144" 12, 26 -- Received: from mta2.lbl.gov ([128.3.41.12]) 12, 26 -- by ironport2.lbl.gov with ESMTP; 15 Nov 2007 12:25:57 -0800 12, 26 -- Received: from [131.243.35.239] (0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.239]) 12, 26 -- by mta2.lbl.gov (8.13.8/8.13.8) with ESMTP id lAFKPuiI000719; 12, 26 -- Thu, 15 Nov 2007 12:25:56 -0800 (PST) 12, 26 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov} 12, 26 -- To: jmkrupp-at-ucsc.edu 12, 26 -- In-Reply-To: {200711142352.lAENqshZ004862-at-ns.microscopy.com} 12, 26 -- Subject: Re: [Microscopy] Material characterization ideas 12, 26 -- References: {200711142352.lAENqshZ004862-at-ns.microscopy.com} 12, 26 -- Message-Id: {887932DD-6190-48D5-84AC-54AEFF54B088-at-lbl.gov} 12, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 12, 26 -- Content-Transfer-Encoding: 7bit 12, 26 -- Mime-Version: 1.0 (Apple Message framework v912) 12, 26 -- Date: Thu, 15 Nov 2007 12:25:52 -0800 12, 26 -- Cc: microscopy-at-microscopy.com 12, 26 -- X-Mailer: Apple Mail (2.912) ==============================End of - Headers==============================
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Email: Phil.Ahrenkiel-at-sdsmt.edu Name: Phil Ahrenkiel
Organization: South Dakota School of Mines & Technology
Title-Subject: [Filtered] alternative ultramicrotome knives
Question: I am trying to find a reasonably inexpensive way for students to practice using our ultramicrotome, without the potential for damaging the diamond knife. I have not had any luck making glass knives using tools we have available, and the knife cutters are quite expensive. Plus, the whole process of gluing the boat onto the knife, or using tape, seems a little silly to me. I'm not so concerned with whether the sections are electron-thin at this point, only that we can develop the method.
I was hoping it would be possible to buy cheap steel knives that could fit into the knife holder for our RMC ultramicrotome. This would seem natural, since we already use razor blades to trim the blocks. We just need some way to mount the blades, but I can't find any type of steel knife that fits in the ultramicrotome holder. The other possibilities I have come across are sapphire and tungsten carbide knives. The tungsten carbide knives at least come in a triangular shape, so we just need some tape to make the boat. We can always switch to the diamond knife when we need ultra-thin sections.
I would be very grateful of any suggestions someone out there may have. We are mainly cutting polymeric materials embedded in resin. Maybe the only option is to be more protective of the diamond knives. Thanks.
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I would like to announce our 5th Annual Advanced Imaging Methods Workshop in Berkeley January 16 ñ 18, 2008.
For more information and to register, please follow this link: http://guest.cvent.com/EVENTS/Info/Summary.aspx?e=6f8e24bd-473f-4769-8f3c-3c74aa7ead88
The CRL Molecular Imaging Center, in conjunction with Becker & Hickl GmbH, is proud to host once again the Advanced Imaging Methods workshop on 16-18 January 2008. In collaboration with the CNR Biological Imaging Facility, this yearís workshop will feature 3 full days of seminars and demonstrations on the UC Berkeley main campus.
Topics for this yearís workshop will include, but are not limited to: FRET, multi-photon, fluorescent lifetime imaging microscopy (FLIM), calcium imaging, deep-tissue imaging, spectroscopy, single molecule detection techniques, nanotechnology, high resolution techniques, AFM, x-ray crystallography, data collection and analysis, high content imaging and more.
Please click the links below to view the summary, agenda, and to register or decline. Registration is limited and required prior to the event. Please feel free to forward this invite to colleagues who may find this conference of value.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mcinerne-at-rose-hulman.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mcinerne-at-rose-hulman.edu Name: Michael McInerney
Organization: Rose-Hulman Institute
Title-Subject: [Filtered] Max magnification problems Hitachi S-3000N
Question: Hi
We own an Hitachi S-3000N SEM. We are unable to set the magnification at greater than 750x with the normal working distance. Shortening the working distance allows magnification of up to 1000x. But these numbers are far less than the 15,000x that we once used to get.
We hope that the problem may be quite simple - a software setting for example. But the problem may lie in the fact that the column has not been cleaned for over a year.
Does anyone have any idea why the magnification will not go above 750x no matter how far the knob is twisted?
Michael
Michael McInerney, Rose-Hulman Institute, Terre Haute, IN 47805.
The idea of using glass knives as a less expensive alternative for students is a good one (and also a cheap way to face blocks for anyone!); and buying a used LKB Knifemaker from a place like Ebay or Labx can be a good alternative.
But, "caveat emptor:" The older LKB Knifemakers are good machines, but they are compact, heavy beasts. Because of that it's very easy for the shipper to unknowingly "underpack" them, using a cheap box and/or not enough packing material. We have a service that overhauls these knifemakers (we are not the only ones); we discovered this the hard way on the first few jobs we did, the first one or two came pretty badly banged up shipping. We now have a special crate we use for transit. I would strongly encourage you to make clear specifications to the shipper for a sturdy box and lots of layers of packing material.
Furthermore: These Knifemakers, despite being built like a brick house, eventually do need to be rebuilt; new scoring blades and such. It's possible that the seller on Ebay or Labx does not know anything about how to use the machine, much less it's condition. Lots of those older machines were used for a long time until they became unusable. They often weren't fixed because the original manufacturer no longer exists, and the units were then mothballed.
So it's possible that the unit you buy used won't work properly, and to get it to work you will have to spend about as much money again getting it overhauled, back into working order. This would increase the cost beyond the acquisition cost.
*** Vendor Disclaimer: This note was written by a vendor who is warning you about possible extra costs, which may cause you to decide against purchasing an old LKB Knifemaker even though the company stands to benefit from overhaul work. ***
Best Regards,
Tom Pella General Manager Ted Pella, Inc. 4595 Mountain Lakes Blvd. Redding, CA 96003 Tel: 800.237.3526 Fax: 530.243.3761 http://www.tedpella.com
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: Wednesday, November 14, 2007 12:29 PM To: tom_pella-at-tedpella.com
Phil,
Also, check out LabX, a company that sells used laboratory equipment.
I noticed several knifemakers in the $700-800 range. Probably you can negotiate a better price.
Here is a link: www.labx.com/v2/newad.cfm?CatID=39
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
To obtain the full magnification range on an instrument there is often a relay that comes in at some point; perhaps the relay is not making contact or it has failed?
Steve Chapman Protrain for EM training & consultancy world wide Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
----- Original Message ----- X-from: {mcinerne-at-rose-hulman.edu} To: {protrain-at-emcourses.com} Sent: Friday, November 16, 2007 2:50 AM
This Question was submitted to Ask-A-Microscopist by (exploratorium-at-tiscali.it) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, November 16, 2007 at 07:10:26 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both exploratorium-at-tiscali.it as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: exploratorium-at-tiscali.it Name: giovanni de caro
Organization: exploratorium luigi montalbÚ
Education: Graduate College
Location: Campobasso, Italia
Title: video tutorial on AMRAY 1000 SEM or similar SEM
Question: We are looking for a video tutorial on the operation of AMRAY 1000 SEM or similar unit. We know that such old videotapes on VHS or video 8 tape were diffused by AMRAY but to date we have been unable to have one. We are trying to restart an old AMRAY 1000 SEM for our science museum for youngsters in Italy (see our website: http://web.tiscali.it/exploratorium). Of course we would pay for shipping costs if requested , as well as for costs to duplicate the videotape or (better) to copy it on a CD or DVD. Thank you for your kind help. Giovanni De Caro, MD Italia
I have an S-3000N so I thought I would check my values for magnifications.
I assume that you mean that when you turn the magnification control that the maximum value that you can reach is either 750x or 1000x after which more turning in the same direction results in the beeping sound when you've turned the magn digital encoder knob too far. Furthermore I also assume that the magnification readout at the top of the screen and on the black data bar have the same value and the scale bar doesn't change once you've reached the maximum.
If all of this is true then maybe my readings will help: at 30kv WD 3mm magn range is x100 to x300k; WD 60mm magn range x15 to x60k at 20kv WD 3mm magn range is x90 to x300k; WD 60mm magn range x15 to x60k at 5kv WD 3mm magn range is x60 to x200k; WD 60mm magn range x15 to x45k at0.3kv WD 3mm magn range is x50 to x50k; WD 60mm magn range x15 to x10k
so the lowest magn is between x15 and x100 depending on WD and kv and the highest magn is between x10k and x300k depending on WD and kv
What worries me a little is that you quote a value of 15k as a previous best where I would have to be operating at 1kv and maximum WD of 60mm to get such a low value.
If you are simply talking about poor resolution rather than max magnification values then ignore this.
Sorry if I've got the wrong end of the stick but it might be worth you checking a range of min max magnifications for different kvs and WDs.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: mcinerne-at-rose-hulman.edu
Dear Michael, I also have a S-3000N and I have never seen a problem like that on it. Can you try changing the magnification by mouse-clicking in the magnification window? Right-click to go down and left-click to go up. This will tell you if it is the control box or the software. Are you in an unusual mode? Is the Beam Control/Condenser lens in "Normal" mode? Is your "scan" control in "Run" or "Freeze" or is the "X-ray Analysis Mode" on? My usual way to fix any problems that show up is to shut down the software, shut down the computer and turn the "DISPLAY" switch off when the computer says: "It is now safe to turn off your computer" (Windows NT on my S3000N) Turn on the "DISPLAY" switch to turn on the system and reboot the computer. If that doesn't work you may have to get the system serviced and the software re-installed by Hitachi Service. Good luck and let us know what works. Regards,
-----Original Message----- X-from: mcinerne-at-rose-hulman.edu [mailto:mcinerne-at-rose-hulman.edu] Sent: November 15, 2007 7:03 PM To: maryflet-at-interchange.ubc.ca
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Email: mcinerne-at-rose-hulman.edu Name: Michael McInerney
Organization: Rose-Hulman Institute
Title-Subject: [Filtered] Max magnification problems Hitachi S-3000N
Question: Hi
We own an Hitachi S-3000N SEM. We are unable to set the magnification at greater than 750x with the normal working distance. Shortening the working distance allows magnification of up to 1000x. But these numbers are far less than the 15,000x that we once used to get.
We hope that the problem may be quite simple - a software setting for example. But the problem may lie in the fact that the column has not been cleaned for over a year.
Does anyone have any idea why the magnification will not go above 750x no matter how far the knob is twisted?
Michael
Michael McInerney, Rose-Hulman Institute, Terre Haute, IN 47805.
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Title-Subject: [Filtered] Photooxidation of GFP for EM
Question: Has anyone used the technique for photooxidation of GFP protein cited in Nature Methods vol2 No11 November 2005? We would like to localize myelinated GFP cells in a co-culture system. We have a number of controls that would need to be done at the same time as the experimental. Since each sample needs to be photobleached on a microscope it seems there could be considerable variablity between samples. Is there any way to guarantee that all the samples will be treated in the same manner? Is there another method to photobleach all the samples simultaneously? Also, can this technique be done on Thermonox coverslips? The protocol suggest using glass coverslips and Hydrofluoric acid after polymerization is complete. Thanks for your help.
This Question was submitted to Ask-A-Microscopist by (cookrn-at-muohio.edu) from http://microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, November 16, 2007 at 14:47:59 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both cookrn-at-muohio.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: I am working on an independent study project to analyze the differences in lignin quantities between the roots and the stems cut from the four cardinal directions from different tree genera. I have taken high resolution micrographs of sections that I cut and stained, but am having trouble finding an adequate solution to stitching the photos together. I have toyed with Hugin and PTAssembler, which both utilize the open-source Panorama Tools library, Autopano, and Enblend. Yet, I am either not using these tools correctly, or they are not adequate for what I want to do. I have also heard that ImageJ might also be decent tool to use with the TrakEM2 plugin. On average, I have approximately 20-40 800x600 pixel uncompressed TIFF images being stitched in a grid fashion, which make up the entire image of the section. My question is whether you are familiar with these tools and their abilities. Are you aware if they could perform such a task? Do you have any suggestions as far as where to look for information on this topic? Any help, of course, would be greatly appreciated. Best regards,
SAMPLE: Macrophages infected with a TB strain & controls
PURPOSE: Immuno EM colocalization of a TB protein on macrophage phagolysosome
First primary: Sheep polyclonal First Secondary: donkey anti sheep of bigger particle size
Second primary: Rabbit polyclonal Second Rabbit polyclonal goat-anti-rabbit of smaller particle size
QUESTIONS: (1) Dilution of primary and secondary: any suggestion?
(2) What blocker(s) are best? Or BSA OK as long as Tween 20 is included?
(3) Would cryo EM be the best answer for any membrane and signal? (I have tried using LR Gold post embedding method.)
(4) Fixation protocol to suggest
(5) Safety precaution
Any suggestion(s) or experience to share would be greatly appreciated. Replies online or offline is fine.
Sincerely, Fanny Chu Ultrastructural Imaging The James C Hogg iCAPTURE Lab, University of British Columbia, St. Paul's Hospital Site Rm 166, 1081 Burrard St, Vancouver, BC, Canada (604) 806-8346, x62712, x62703
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==============================Original Headers============================== 15, 17 -- From FChu-at-mrl.ubc.ca Fri Nov 16 17:06:45 2007 15, 17 -- Received: from mail.mrl.ubc.ca (mrl.ubc.ca [137.82.67.155]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAGN6jj2022778 15, 17 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Nov 2007 17:06:45 -0600 15, 17 -- Received: from mrlmail-MTA by mail.mrl.ubc.ca 15, 17 -- with Novell_GroupWise; Fri, 16 Nov 2007 15:06:43 -0800 15, 17 -- Message-Id: {473DB1DD.EB79.00BC.0-at-mrl.ubc.ca} 15, 17 -- X-Mailer: Novell GroupWise Internet Agent 7.0.1 15, 17 -- Date: Fri, 16 Nov 2007 15:06:06 -0800 15, 17 -- From: "Fanny Chu" {FChu-at-mrl.ubc.ca} 15, 17 -- To: {Microscopy-at-microscopy.com} 15, 17 -- Subject: TEM co-localization of TB infected macrophages 15, 17 -- Mime-Version: 1.0 15, 17 -- Content-Type: text/plain; charset=US-ASCII 15, 17 -- Content-Disposition: inline 15, 17 -- Content-Transfer-Encoding: 8bit 15, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lAGN6jj2022778 ==============================End of - Headers==============================
The newest version of Photoshop has a nice feature that stitches together images. I have been using it extensively with LM brightfield immunofluorescence (4-14 images each about 1.3 MB). only problem is that it won't groups in batches. You have to select each set which is annoying when you have 20 sets of images. But it works well. Tom
At 04:48 PM 11/16/07, you wrote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
Photoshop CS would do if you have enough overlapping features (or you have to manually stitch some as the algorithm might fail). We used to stitch routinely 100s of images if illumination was correctly properly.
If any distortion is present in your images, you might try Autostitch. But I do not remember if it takes Tiff files.
Good luck.
Xuejun
Xue-jun Sun, Ph.D. Dept. Exp. Oncology Cross Cancer Institute 11560 University Ave, Edmonton Alberta T6G 1Z2 Canada Phone: (780) 432-8898 Fax: (780) 432-8425
-----Original Message----- X-from: cookrn-at-muohio.edu [mailto:cookrn-at-muohio.edu] Sent: Friday, November 16, 2007 3:54 PM To: xjsun-at-ualberta.ca
This Question was submitted to Ask-A-Microscopist by (cookrn-at-muohio.edu) from http://microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, November 16, 2007 at 14:47:59 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both cookrn-at-muohio.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: I am working on an independent study project to analyze the differences in lignin quantities between the roots and the stems cut from the four cardinal directions from different tree genera. I have taken high resolution micrographs of sections that I cut and stained, but am having trouble finding an adequate solution to stitching the photos together. I have toyed with Hugin and PTAssembler, which both utilize the open-source Panorama Tools library, Autopano, and Enblend. Yet, I am either not using these tools correctly, or they are not adequate for what I want to do. I have also heard that ImageJ might also be decent tool to use with the TrakEM2 plugin. On average, I have approximately 20-40 800x600 pixel uncompressed TIFF images being stitched in a grid fashion, which make up the entire image of the section. My question is whether you are familiar with these tools and their abilities. Are you aware if they could perform such a task? Do you have any suggest! ions as far as where to look for information on this topic? Any help, of course, would be greatly appreciated. Best regards,
==============================Original Headers============================== 10, 13 -- From zaluzec-at-ultra5.microscopy.com Fri Nov 16 16:47:36 2007 10, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 10, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAGMlZqF032140 10, 13 -- for {microscopy-at-microscopy.com} ; Fri, 16 Nov 2007 16:47:36 -0600 10, 13 -- Mime-Version: 1.0 10, 13 -- Message-Id: {p06240801c363ce71bdf6-at-[206.69.208.22]} 10, 13 -- Date: Fri, 16 Nov 2007 16:47:34 -0600 10, 13 -- To: microscopy-at-microscopy.com 10, 13 -- From: cookrn-at-muohio.edu (by way of Ask-A-Microscopist) 10, 13 -- Subject: AskAMicroscopist: Stitching High-Resolution Microscope Images 10, 13 -- Content-Type: text/plain; charset="us-ascii" 10, 13 -- Content-Transfer-Encoding: 8bit 10, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lAGMlZqF032140 ==============================End of - Headers==============================
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==============================Original Headers============================== 23, 22 -- From xjsun-at-ualberta.ca Fri Nov 16 18:15:44 2007 23, 22 -- Received: from mailgw1.cancerboard.ab.ca (mx1.cancerboard.ab.ca [216.123.198.11]) 23, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAH0FhEg015729 23, 22 -- for {microscopy-at-microscopy.com} ; Fri, 16 Nov 2007 18:15:43 -0600 23, 22 -- Reply-To: {xjsun-at-ualberta.ca} 23, 22 -- From: "Xuejun Sun" {xjsun-at-ualberta.ca} 23, 22 -- To: {microscopy-at-microscopy.com} 23, 22 -- Cc: {cookrn-at-muohio.edu} 23, 22 -- References: {200711162254.lAGMs11B013609-at-ns.microscopy.com} 23, 22 -- Subject: RE: [Microscopy] AskAMicroscopist: Stitching High-Resolution Microscope Images 23, 22 -- Date: Thu, 16 Nov 2006 17:15:42 -0700 23, 22 -- Organization: UA 23, 22 -- Message-ID: {000c01c709dd$84cae3e0$29e966a4-at-ad.cancerboard.ab.ca} 23, 22 -- MIME-Version: 1.0 23, 22 -- Content-Type: text/plain; 23, 22 -- charset="iso-8859-1" 23, 22 -- Content-Transfer-Encoding: 7bit 23, 22 -- X-Mailer: Microsoft Office Outlook 11 23, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 23, 22 -- Thread-Index: Acgoo5VRYjw3ddLzQ9SZ7lteq9fO5QABfsxg 23, 22 -- In-Reply-To: {200711162254.lAGMs11B013609-at-ns.microscopy.com} 23, 22 -- X-OriginalArrivalTime: 17 Nov 2007 00:15:42.0879 (UTC) FILETIME=[FDDFEAF0:01C828AE] ==============================End of - Headers==============================
Ryan: When I am not taking pictures with a TEM I am taking them with a camera. I have been doing a number of panoramas lately that are combined from multiple images in multiple rows. So far my largest image is over 900MB and it was stitched together from three rows of seven images, each being about 20MB, 4200pix X 2800pix each. Photoshop CS3 has really improved the stitching process over the earlier versions. And yes, you do have to have some overlap in the images. I have been stitching each row separately and then I stitch all of the rows together. With smaller images like what you have it might be worth a try to see if PS can do it all at one time. I expected to have to do a lot of tweaking but if you have a proper overlap that the program can recognize the program can handle it automatically. Of course, this doesn't go very fast and I have hung up my computer numerous times but it does get the job done. I am not going to go into the details here unless someone is interested. Just shoot me an email if you are.
Norm Olson
} } Email: cookrn-at-muohio.edu } Name: Ryan Cook } } Organization: Miami University (Ohio) } } Education: Undergraduate College } } Location: Oxford, OH, USA } } Title: Stitching High-Resolution Microscope Images } } Question: I am working on an independent study project to analyze the } differences in lignin quantities between the roots and the stems cut from } the four cardinal directions from different tree genera. I have taken high } resolution micrographs of sections that I cut and stained, but am having } trouble finding an adequate solution to stitching the photos together. I } have toyed with Hugin and PTAssembler, which both utilize the open-source } Panorama Tools library, Autopano, and Enblend. Yet, I am either not using } these tools correctly, or they are not adequate for what I want to do. I } have also heard that ImageJ might also be decent tool to use with the } TrakEM2 plugin. On average, I have approximately 20-40 800x600 pixel } uncompressed TIFF images being stitched in a grid fashion, which make up the } entire image of the section. My question is whether you are familiar with } these tools and their abilities. Are you aware if they could perform such a } task? Do you have any suggest! } ions as far as where to look for information on this topic? Any help, of } course, would be greatly appreciated. Best regards, } } Ryan Cook
--
______________________________________________________________ Norm Olson Cryoelectron Microscopy Facilities Manager 1510 Bonner Hall Department of Chemistry & Biochemistry, MC-0378 University of California San Diego La Jolla, CA 92093-0378 nholson-at-ucsd.edu http://cryoem.ucsd.edu Cell: (858)220-2183 (858)822-6718 - Office; (858)534-5846 - Fax ______________________________________________________________
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Ryan, Try PTGui software (http://www.ptgui.com) (at least their demo version which is free for one month). If your images do not overlap sufficiently, you can manually choose corresponding features which simultaneously appear in two adjacent images. It is easy to do if you correctly arrange yours images in the right order using the "Source images" function.
Prof. Jean-Paul Baïlon, Program of Materials Eng. Ecole Polytechnique de Montreal
Ryan, Try PTGui software (http://www.ptgui.com) (at least their demo version which is free for one month). If your images do not overlap sufficiently, you can manually choose corresponding features which simultaneously appear in two adjacent images. It is easy to do if you correctly arrange your images in the right order using the "Source images" function.
Prof. Jean-Paul Baïlon, Program of Materials Eng. Ecole Polytechnique de Montreal
---------------------------------------- } Ryan: When I am not taking pictures with a TEM I am taking them with a camera. I have been doing a number of } panoramas lately that are combined from multiple images in multiple rows. So far my largest image is over 900MB } and it was stitched together from three rows of seven images, each being about 20MB, 4200pix X 2800pix each. } Photoshop CS3 has really improved the stitching process over the earlier versions. And yes, you do have to have } some overlap in the images. I have been stitching each row separately and then I stitch all of the rows } together. With smaller images like what you have it might be worth a try to see if PS can do it all at one time. } I expected to have to do a lot of tweaking but if you have a proper overlap that the program can recognize the
} Program can handle it automatically. Of course, this doesn't go very fast and I have hung up my computer } numerous times but it does get the job done. I am not going to go into the details here unless someone is } interested. Just shoot me an email if you are.
} Norm Olson
} } Email: cookrn-at-muohio.edu } Name: Ryan Cook } } Organization: Miami University (Ohio) } } Education: Undergraduate College } } Location: Oxford, OH, USA } } Title: Stitching High-Resolution Microscope Images } } Question: I am working on an independent study project to analyze the } differences in lignin quantities between the roots and the stems cut } from the four cardinal directions from different tree genera. I have } taken high resolution micrographs of sections that I cut and stained, } but am having trouble finding an adequate solution to stitching the } photos together. I have toyed with Hugin and PTAssembler, which both } utilize the open-source Panorama Tools library, Autopano, and Enblend. } Yet, I am either not using these tools correctly, or they are not } adequate for what I want to do. I have also heard that ImageJ might } also be decent tool to use with the } TrakEM2 plugin. On average, I have approximately 20-40 800x600 pixel } uncompressed TIFF images being stitched in a grid fashion, which make } up the entire image of the section. My question is whether you are } familiar with these tools and their abilities. Are you aware if they } could perform such a task? Do you have any suggest! } ions as far as where to look for information on this topic? Any } help, of course, would be greatly appreciated. Best regards, } } Ryan Cook
==============================Original Headers============================== 15, 21 -- From jean-paul.bailon-at-polymtl.ca Sat Nov 17 17:27:40 2007 15, 21 -- Received: from smtp.polymtl.ca (smtp.polymtl.ca [132.207.4.11]) 15, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAHNResk001484 15, 21 -- for {Microscopy-at-microscopy.com} ; Sat, 17 Nov 2007 17:27:40 -0600 15, 21 -- Received: from SousSol (bas4-montreal28-1279577933.dsl.bell.ca [76.68.207.77]) 15, 21 -- by smtp.polymtl.ca (8.13.8/8.13.8) with ESMTP id lAHNRZX8009849 15, 21 -- for {Microscopy-at-microscopy.com} ; Sat, 17 Nov 2007 18:27:39 -0500 15, 21 -- From: =?iso-8859-1?Q?Jean-Paul_Ba=EFlon?= {jean-paul.bailon-at-polymtl.ca} 15, 21 -- To: {Microscopy-at-microscopy.com} 15, 21 -- Subject: RE: AskAMicroscopist: Stitching High-Resolution Microscope Images 15, 21 -- Date: Sat, 17 Nov 2007 18:27:21 -0500 15, 21 -- Message-ID: {000301c82971$69d15970$8800a8c0-at-SousSol} 15, 21 -- MIME-Version: 1.0 15, 21 -- Content-Type: text/plain; 15, 21 -- charset="iso-8859-1" 15, 21 -- X-Mailer: Microsoft Office Outlook 11 15, 21 -- Thread-Index: AcgpcWbnZKWTVSLlSiuNobLOAyD4sg== 15, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 15, 21 -- X-Poly-FromMTA: (bas4-montreal28-1279577933.dsl.bell.ca [76.68.207.77]) at Sat, 17 Nov 2007 23:27:35 +0000 15, 21 -- Content-Transfer-Encoding: 8bit 15, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lAHNResk001484 ==============================End of - Headers==============================
} If any distortion is present in your images, you might try } Autostitch. But I do not remember if it takes Tiff files.
Autostitch has long been a favorite for photographers stitching panoramas. The demo works with JPEGs only, but it should be easy to convert for a trial. The demo is available here: http://www.cs.ubc.ca/~mbrown/autostitch/autostitch.html
If it is satisfactory the same webpage refers to several softwares that have licensed the algorithm.
hth & Genuinely, Michael Shaffer :o)
SEM/MLA Research Coordinator http://www.mun.ca/creait/maf/ Inco Innovation Centre Memorial University St. John's, Newfoundland
} -------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } } This Question was submitted to Ask-A-Microscopist by } (cookrn-at-muohio.edu) } from } http://microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on } Friday, November 16, 2007 at 14:47:59 } Remember to consider the Grade/Age of the student when considering the } Question } -------------------------------------------------------------- } ------------- } Please reply to both cookrn-at-muohio.edu as well as to the Microscopy } Listserver } -------------------------------------------------------------- } ------------- } } Email: cookrn-at-muohio.edu } Name: Ryan Cook } } Organization: Miami University (Ohio) } } Education: Undergraduate College } } Location: Oxford, OH, USA } } Title: Stitching High-Resolution Microscope Images } } Question: I am working on an independent study project to analyze the } differences in lignin quantities between the roots and the } stems cut from } the four cardinal directions from different tree genera. I } have taken high } resolution micrographs of sections that I cut and stained, } but am having } trouble finding an adequate solution to stitching the photos } together. I } have toyed with Hugin and PTAssembler, which both utilize the } open-source } Panorama Tools library, Autopano, and Enblend. Yet, I am } either not using } these tools correctly, or they are not adequate for what I } want to do. I } have also heard that ImageJ might also be decent tool to use with the } TrakEM2 plugin. On average, I have approximately 20-40 800x600 pixel } uncompressed TIFF images being stitched in a grid fashion, } which make up the } entire image of the section. My question is whether you are } familiar with } these tools and their abilities. Are you aware if they could } perform such a } task? Do you have any suggest! } ions as far as where to look for information on this topic? } Any help, of } course, would be greatly appreciated. Best regards, } } Ryan Cook } } -------------------------------------------------------------- } ------------- } } } ==============================Original } Headers============================== } 10, 13 -- From zaluzec-at-ultra5.microscopy.com Fri Nov 16 16:47:36 2007 } 10, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 10, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id } lAGMlZqF032140 } 10, 13 -- for {microscopy-at-microscopy.com} ; Fri, 16 Nov } 2007 16:47:36 } -0600 } 10, 13 -- Mime-Version: 1.0 } 10, 13 -- Message-Id: {p06240801c363ce71bdf6-at-[206.69.208.22]} } 10, 13 -- Date: Fri, 16 Nov 2007 16:47:34 -0600 } 10, 13 -- To: microscopy-at-microscopy.com } 10, 13 -- From: cookrn-at-muohio.edu (by way of Ask-A-Microscopist) } 10, 13 -- Subject: AskAMicroscopist: Stitching } High-Resolution Microscope } Images } 10, 13 -- Content-Type: text/plain; charset="us-ascii" } 10, 13 -- Content-Transfer-Encoding: 8bit } 10, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id lAGMlZqF032140 } ==============================End of - } Headers============================== } } } This e-mail and any attachments may contain confidential and } privileged information. If you are not the intended recipient, } please notify the sender immediately by return e-mail, delete this } e-mail and destroy any copies. Any dissemination or use of this } information by a person other than the intended recipient is } unauthorized and may be illegal. } } ==============================Original } Headers============================== } 23, 22 -- From xjsun-at-ualberta.ca Fri Nov 16 18:15:44 2007 } 23, 22 -- Received: from mailgw1.cancerboard.ab.ca } (mx1.cancerboard.ab.ca [216.123.198.11]) } 23, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id lAH0FhEg015729 } 23, 22 -- for {microscopy-at-microscopy.com} ; Fri, 16 Nov } 2007 18:15:43 -0600 } 23, 22 -- Reply-To: {xjsun-at-ualberta.ca} } 23, 22 -- From: "Xuejun Sun" {xjsun-at-ualberta.ca} } 23, 22 -- To: {microscopy-at-microscopy.com} } 23, 22 -- Cc: {cookrn-at-muohio.edu} } 23, 22 -- References: {200711162254.lAGMs11B013609-at-ns.microscopy.com} } 23, 22 -- Subject: RE: [Microscopy] AskAMicroscopist: } Stitching High-Resolution Microscope Images } 23, 22 -- Date: Thu, 16 Nov 2006 17:15:42 -0700 } 23, 22 -- Organization: UA } 23, 22 -- Message-ID: } {000c01c709dd$84cae3e0$29e966a4-at-ad.cancerboard.ab.ca} } 23, 22 -- MIME-Version: 1.0 } 23, 22 -- Content-Type: text/plain; } 23, 22 -- charset="iso-8859-1" } 23, 22 -- Content-Transfer-Encoding: 7bit } 23, 22 -- X-Mailer: Microsoft Office Outlook 11 } 23, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 } 23, 22 -- Thread-Index: Acgoo5VRYjw3ddLzQ9SZ7lteq9fO5QABfsxg } 23, 22 -- In-Reply-To: {200711162254.lAGMs11B013609-at-ns.microscopy.com} } 23, 22 -- X-OriginalArrivalTime: 17 Nov 2007 00:15:42.0879 } (UTC) FILETIME=[FDDFEAF0:01C828AE] } ==============================End of - } Headers============================== } } __________ NOD32 2665 (20071117) Information __________ } } This message was checked by NOD32 antivirus system. } http://www.eset.com } }
==============================Original Headers============================== 10, 28 -- From michael-at-shaffer.net Sun Nov 18 06:24:59 2007 10, 28 -- Received: from n007.sc1.he.tucows.com (fh1020.dia.he.tucows.com [64.97.168.30]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAICOwl2011586 10, 28 -- for {Microscopy-at-microscopy.com} ; Sun, 18 Nov 2007 06:24:58 -0600 10, 28 -- Received: from sc1-out07.emaildefenseservice.com (64.97.139.2) by n007.sc1.he.tucows.com (7.2.069.1) 10, 28 -- id 470D104800838C42 for Microscopy-at-microscopy.com; Sun, 18 Nov 2007 12:24:57 +0000 10, 28 -- X-SpamScore: 50 10, 28 -- X-Spamcatcher-Summary: 50,0,0,28ac09fa5e18f126,b8918717ed728366,michael-at-shaffer.net,-,RULES_HIT:2:10:69:355:379:539:541:542:599:601:901:945:960:967:973:980:988:989:1155:1156:1160:1260:1277:1311:1313:1314:1345:1359:1437:1515:1516:1518:1535:1593:1594:1605:1730:1747:1766:1792:2075:2078:2194:2197:2198:2199:2200:2201:2288:2378:2393:2525:2551:2553:2561:2568:2627:2682:2685:2741:2771:2773:2829:2857:2859:2892:2913:2933:2937:2939:2942:2945:2947:2951:2954:3022:3027:3357:3421:3571:3636:3653: 10, 28 -- 3865:3866:3867:3868:3869:3870:3871:3872:3873:3874:3934:3936:3938:3941:3944:4049:4119:4250:4321:4384:4470:4647:4860:5007:6117:6119:6248:6261:7576:7679:7688,0,RBL:none,CacheIP:none,Bayesian:0.5,0.5,0.5,Netcheck:none,DomainCache:0,MSF:not bulk,SPF:,MSBL:none,DNSBL:none 10, 28 -- X-Spamcatcher-Explanation: (51%) OBFUSCATED_WORD1_ONLINE;(-49%) BODY: contains likely legitimate use of "online"; 10, 28 -- Received: from rarewolf (CPE00c0f0237e36-CM0018682b4b4c.cpe.net.cable.rogers.com [205.251.83.78]) 10, 28 -- (Authenticated sender: michael-at-shaffer.net) 10, 28 -- by sc1-out07.emaildefenseservice.com (Postfix) with ESMTP 10, 28 -- for {Microscopy-at-microscopy.com} ; Sun, 18 Nov 2007 12:24:54 +0000 (UTC) 10, 28 -- From: "michael shaffer" {michael-at-shaffer.net} 10, 28 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com} 10, 28 -- References: {200711170016.lAH0GF5r016345-at-ns.microscopy.com} 10, 28 -- Subject: RE: [Microscopy] RE: AskAMicroscopist: Stitching High-Resolution Microscope Images 10, 28 -- Date: Sun, 18 Nov 2007 08:54:48 -0330 10, 28 -- Message-ID: {001501c829de$074c7970$4701a8c0-at-rarewolf} 10, 28 -- MIME-Version: 1.0 10, 28 -- Content-Type: text/plain; 10, 28 -- charset="us-ascii" 10, 28 -- Content-Transfer-Encoding: 7bit 10, 28 -- X-Mailer: Microsoft Office Outlook 11 10, 28 -- Thread-Index: AcgorjBPvYtRkcqjRYOo5BZ6rTF+BABLdaag 10, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 10, 28 -- In-Reply-To: {200711170016.lAH0GF5r016345-at-ns.microscopy.com} ==============================End of - Headers==============================
Dear Fanny- I don't have suggestions about the EM immuno protocol, but, having worked with a group here that studies TB I can foresee an issue: Any infected/infective materials cannot leave the level 3 bio-containment lab unless the pathogen has been totally "neutralized" (killed). For our structural studies, this required a 3 hour fixation in 2.5% glutaraldehyde. That being said, I suspect that you will have to come up with a protocol for doing at least your primary Ab labellings in the samples while they are alive and in the BCL3 unit, fixing them, and then doing the secondaries afterward, either pre-embedding, or on the embedded, sectioned material. Please post your solution to the List so we can all benefit from it. Good luck, Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Weill Cornell Medical College
Our new EDS system is nearing the end of warranty. My faculty supervisor would like to know if it is worth the money to purchase a service contract . I would like to hear, privately of course, from actual users of EDS systems about your experience and opinions on whether spending the money for a service contract is worth the expenditure.
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 5, 20 -- From tbargar-at-unmc.edu Mon Nov 19 11:07:21 2007 5, 20 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAJH7LBm024965 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 19 Nov 2007 11:07:21 -0600 5, 20 -- Received: from zixvpm01.unmc.edu (ZixVPM [127.0.0.1]) 5, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id ECEF74C0CE 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 19 Nov 2007 11:07:20 -0600 (CST) 5, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu [137.197.103.35]) 5, 20 -- by zixvpm01.unmc.edu (Proprietary) with ESMTP id AE0CC4C07E 5, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 19 Nov 2007 11:07:19 -0600 (CST) 5, 20 -- Subject: Service contracts on EDS systems 5, 20 -- To: Microscopy-at-MSA.Microscopy.Com 5, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 5, 20 -- Message-ID: {OF3A8EAECD.DF9BD081-ON86257398.005D933B-86257398.005E0D85-at-unmc.edu} 5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 5, 20 -- Date: Mon, 19 Nov 2007 11:07:18 -0600 5, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 11/19/2007 11:07:19 5, 20 -- AM 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
We're in the fortunate position of looking around at acquiring a confocal microscope sometime in 2008. Before we start hearing the pitches from the manufacturers, we'd like to hear from the "real" world. Specifically, what you have and what you think of it (ease-of-use, upgrades, level of control, service and support, etc.). After having a look around, it seems like the two contenders for us are the Zeiss 510 META NLO and the Leica TCS SP5. Flexibility is key for us, as it's going to have a fairly broad set of uses as both a research tool and as a development tool. Cost isn't critical at this point; we're basically being told to "get what we need". While we have people with confocal experience, each is
a little biased that the system they know is the best one.
Thanks much! -Chris
==============================Original Headers============================== 5, 29 -- From christopher.hayden-at-novartis.com Mon Nov 19 12:23:16 2007 5, 29 -- Received: from mail194.messagelabs.com (mail194.messagelabs.com [85.158.140.211]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAJINGAI008522 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 12:23:16 -0600 5, 29 -- X-VirusChecked: Checked 5, 29 -- X-Env-Sender: christopher.hayden-at-novartis.com 5, 29 -- X-Msg-Ref: server-10.tower-194.messagelabs.com!1195496595!1972703!1 5, 29 -- X-StarScan-Version: 5.5.12.14.2; banners=-,-,- 5, 29 -- X-Originating-IP: [160.62.1.174] 5, 29 -- Received: (qmail 15936 invoked from network); 19 Nov 2007 18:23:15 -0000 5, 29 -- Received: from ch2ssaenov01.novartis.com (HELO ch2ssaenov01.novartis.com) (160.62.1.174) 5, 29 -- by server-10.tower-194.messagelabs.com with AES256-SHA encrypted SMTP; 19 Nov 2007 18:23:15 -0000 5, 29 -- Received: from ch1ssainov01.novartis.net (ch1ssainov01s [192.37.2.173]) 5, 29 -- by ch2ssaenov01.novartis.com (8.13.8/8.13.8) with ESMTP id lAJINE00017372 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 19:23:14 +0100 5, 29 -- Received: from phchbs-s3140.EU.novartis.net (phchbs-s3140.eu.novartis.net [192.37.31.248]) 5, 29 -- by ch1ssainov01.novartis.net (8.13.8/8.13.8) with ESMTP id lAJINE7X016212 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 19:23:14 +0100 5, 29 -- To: Microscopy-at-Microscopy.Com 5, 29 -- Subject: LM - Confocal Opinions 5, 29 -- MIME-Version: 1.0 5, 29 -- X-Mailer: Lotus Notes Release 7.0.1 HF517 January 12, 2007 5, 29 -- Message-ID: {OFC44BD91C.2AD7BFB4-ON85257398.0064ECC4-85257398.006500CF-at-ah.novartis.com} 5, 29 -- From: christopher.hayden-at-novartis.com 5, 29 -- Date: Mon, 19 Nov 2007 13:23:13 -0500 5, 29 -- X-MIMETrack: Serialize by Router on CHBSSPH0/PH/Novartis(Release 7.0.2FP2 HF144|July 27, 2007) at 5, 29 -- 19.11.2007 19:23:14, 5, 29 -- Serialize complete at 19.11.2007 19:23:14 5, 29 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
The Center for Advanced Research and Technology (CART) at the University of North Texas (UNT) has immediate openings for two postdoctoral fellows specializing in the area of advanced characterization of metallic and hybrid materials. Applicants must have a strong commitment to research, and exhibit the potential to sustain a cutting-edge research program involving nanoscale characterization of interfaces and other microstructural features in the aforementioned materials systems. Specifically the applicant must have demonstrated experience in the use of ALL the following advanced characterization techniques:
1. Three-dimensional atom probe (3DAP) tomography including experience in analysis and interpretation of results. 2. Analytical and high-resolution transmission electron microscopy (TEM). 3. Preparation of site-specific samples for 3DAP and TEM using focused ion beam (FIB) milling techniques, such as the use of a dual-beam FIB. 4. Scanning electron microscopy (SEM) including electron backscatter diffraction (EBSD) based orientation imaging microscopy (OIM).
In addition, the applicants are required to have demonstrated research experience in the field of microstructural evolution in nickel base alloys or titanium base alloys or hybrid/composite materials. The required qualifications for all applicants include a Ph.D. in materials science and engineering.
The positions are available for one year with a possible extension based upon mutual agreement and the availability of funding.
UNT, the fourth largest university in Texas, is located in Denton, Texas 35 miles north of the Dallas/Fort Worth metropolitan area. There are approximately 34,000 students registered in 93 bachelors, 111 masters and 50 doctoral degree programs. This is an area of more than six million people, with significant economic growth, numerous industrial establishments, and excellent school districts. This area and the university provide excellent cultural and educational opportunities as well as exceptional employment opportunities for spouses. Applicants should submit a curriculum vitae and the names of at least three references by email (single PDF file) to Dr. Rajarshi Banerjee (Banerjee-at-egw.unt.edu) or Dr. J. D. Luttmer (Luttmer-at-unt.edu). To receive full consideration applications must be received by January 15, 2008.
UNT is an Affirmative Action/Equal Opportunity Employer.
==============================Original Headers============================== 7, 23 -- From drd0036-at-unt.edu Mon Nov 19 15:25:40 2007 7, 23 -- Received: from ironport1.nscmail.private.unt.edu (mailhost.unt.edu [129.120.188.67]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAJLPdLn028529 7, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Nov 2007 15:25:39 -0600 7, 23 -- X-ExtLoopCount1: 1 7, 23 -- X-IronPort-AV: E=Sophos;i="4.21,438,1188795600"; 7, 23 -- d="scan'208";a="3581966" 7, 23 -- Received: from mtsc-d2569gd1.mtsc.eng.unt.edu (HELO mtscD2569GD1) ([129.120.21.110]) 7, 23 -- by ironport1.nscmail.private.unt.edu with ESMTP; 19 Nov 2007 15:25:39 -0600 7, 23 -- From: "Dave Diercks" {drd0036-at-unt.edu} 7, 23 -- To: {Microscopy-at-microscopy.com} 7, 23 -- Subject: Postdoctoral positions at the University of North Texas 7, 23 -- Date: Mon, 19 Nov 2007 15:25:39 -0600 7, 23 -- Message-ID: {003a01c82af2$bb86e050$3294a0f0$-at-edu} 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="US-ASCII" 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- X-Mailer: Microsoft Office Outlook 12.0 7, 23 -- thread-index: Acgq8rk3PR9/1Ms5SSWPetQ/5nHNRA== 7, 23 -- Content-Language: en-us 7, 23 -- x-cr-hashedpuzzle: AFpE ALIt AOBd AQ31 AWwd AqM5 BWff DZor Dqqw FpSR F2ES F2q9 GYPl HF4B Ktai Kz05;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{B94528E9-CADC-4D14-987D-48995FBAE115};ZAByAGQAMAAwADMANgBAAHUAbgB0AC4AZQBkAHUA;Mon, 19 Nov 2007 21:25:35 GMT;UABvAHMAdABkAG8AYwB0AG8AcgBhAGwAIABwAG8AcwBpAHQAaQBvAG4AcwAgAGEAdAAgAHQAaABlACAAVQBuAGkAdgBlAHIAcwBpAHQAeQAgAG8AZgAgAE4AbwByAHQAaAAgAFQAZQB4AGEAcwA= 7, 23 -- x-cr-puzzleid: {B94528E9-CADC-4D14-987D-48995FBAE115} ==============================End of - Headers==============================
The University of North Texas (UNT) seeks research scientists to work in its recently formed Laboratory for Atomic Scale Characterization (LASC), which is part of its new Center for Advanced Research and Technology (CART). CART is a university-wide, multidisciplinary center with an emphasis in the science and engineering disciplines. The LASC research scientists will report to the Facilities Operations Manager and will work with faculty and staff associated with the center and with external users of the facility. The instrumentation list is quite extensive and includes a 3D atom probe, an analytical high resolution TEM, a FIB/FESEM, an environmental SEM, two XRD systems, an AFM and a new Raman system with plans to acquire a new XPS system.
Research Scientist I Qualifications: Applicants for the Research Scientist I positions of the LASC should have considerable experience in the characterization of materials of various types using advanced instrumentation, ideally electron microscopy (SEM and TEM) ion microscopy (3D atom probe tomography), x-ray diffraction and focused ion beam. Applicants should have BS or MS degrees or equivalent experience.
Duties: Candidates will be expected to work with both internal (faculty, post-docs and students) and external (industries, other universities, etc.) customers primarily in a service capacity. They will also be expected to work with the Facilities Operations Manager on all aspects of equipment maintenance and repair, developing appropriate protocols for access and insuring proper documentation of service activities. Finally, candidates will be involved in instrument training and technical assistance for users of general and specialized materials characterization techniques.
Salary: Salary and benefits will be commensurate with the qualifications of the successful candidates. (Salary range is $35k- $60k). These positions are non-tenure track staff positions.
Research Scientist II Qualifications: Applicants for the Research Scientist II positions of the LASC should have considerable experience in the characterization of materials of various types using advanced instrumentation, ideally electron microscopy (SEM and TEM) ion microscopy (3D atom probe tomography), x-ray diffraction and focused ion beam. Applicants should have MS or PhD degrees or equivalent experience.
Duties: Candidates will be expected to work with both internal (faculty, post-docs and students) and external (industries, other universities, etc.) customers primarily in a service capacity. They will also be expected to work with the Facilities Operations Manager on all aspects of equipment maintenance and repair, developing appropriate protocols for access and insuring proper documentation of service activities. Other tasks may include promotion of the facilities through seminars, tours, web page, newsletters and candidates will be expected to participate in professional societies by attending conferences in the field of electron microscopy and materials characterization where they will promote the output of the center. Finally, candidates will be involved in instrument training and technical assistance for users of general and specialized materials characterization techniques and may be asked to assist in teaching undergraduate and graduate laboratories.
Salary: Salary and benefits will be commensurate with the qualifications of the successful candidates. (Salary range is $45k- $75k). These positions are non-tenure track staff positions.
UNT is the fourth largest university in Texas and is strategically located in Denton, Texas 35 miles north of the Dallas/Fort Worth metropolitan area. There are approximately 33,000 students registered in 93 bachelors, 111 masters and 50 doctoral degree programs. This is an area of more than six million people, with significant economic growth, numerous industrial establishments, and excellent school districts.
Applications may be made online at https://jobs.unt.edu. Search under positions in the Materials Science & Engineering department. UNT is an AA/EOE.
==============================Original Headers============================== 13, 24 -- From drd0036-at-unt.edu Mon Nov 19 16:18:52 2007 13, 24 -- Received: from ironport1.nscmail.private.unt.edu (mailhost.unt.edu [129.120.188.67]) 13, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAJMIq7N009681 13, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Nov 2007 16:18:52 -0600 13, 24 -- X-ExtLoopCount1: 1 13, 24 -- X-IronPort-AV: E=Sophos;i="4.21,438,1188795600"; 13, 24 -- d="scan'208";a="3593682" 13, 24 -- Received: from mtsc-d2569gd1.mtsc.eng.unt.edu (HELO mtscD2569GD1) ([129.120.21.110]) 13, 24 -- by ironport1.nscmail.private.unt.edu with ESMTP; 19 Nov 2007 16:18:52 -0600 13, 24 -- From: "Dave Diercks" {drd0036-at-unt.edu} 13, 24 -- To: {Microscopy-at-microscopy.com} 13, 24 -- Subject: Research Scientist positions at the University of North Texas 13, 24 -- Date: Mon, 19 Nov 2007 16:18:51 -0600 13, 24 -- Message-ID: {005001c82afa$2a297610$7e7c6230$-at-edu} 13, 24 -- MIME-Version: 1.0 13, 24 -- Content-Type: text/plain; 13, 24 -- charset="iso-8859-1" 13, 24 -- X-Mailer: Microsoft Office Outlook 12.0 13, 24 -- thread-index: Acgq+ikZ4NADUfsTQsWvmVS6cEs1WA== 13, 24 -- Content-Language: en-us 13, 24 -- x-cr-hashedpuzzle: B4g= AUB8 CfaO DEZT DMiP DYBZ D4gP E6C5 Flsf Gm/7 G8zZ HNt+ HOHF IJja I3rA Jp6H;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{9B261832-B493-4572-9666-2F6C73031F45};ZAByAGQAMAAwADMANgBAAHUAbgB0AC4AZQBkAHUA;Mon, 19 Nov 2007 22:18:49 GMT;UgBlAHMAZQBhAHIAYwBoACAAUwBjAGkAZQBuAHQAaQBzAHQAIABwAG8AcwBpAHQAaQBvAG4AcwAgAGEAdAAgAHQAaABlACAAVQBuAGkAdgBlAHIAcwBpAHQAeQAgAG8AZgAgAE4AbwByAHQAaAAgAFQAZQB4AGEAcwA= 13, 24 -- x-cr-puzzleid: {9B261832-B493-4572-9666-2F6C73031F45} 13, 24 -- Content-Transfer-Encoding: 8bit 13, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lAJMIq7N009681 ==============================End of - Headers==============================
I have no problems with the second posting for positions that was sent to the listserver for this center. It was in the spirit of posting positions here on the Microscopy ListServer. But I can tell you that this particular posting hit on one of my pet peeves buttons, i.e. public advertisement for a position when there are specific individuals already identified. The criteria for the position in this posting is just too specific in the number of techniques required and specific materials required not to be targeted for specific individuals with a newly minted Ph.D. The four techniques are knowledge intensive and then to require specific materials systems to be studied was just too much for me to take and not say anything. Now I know that you have to advertise your positions and state that you are an "Affirmative Action/Equal Opportunity Employer". Now I know that you have to go through the motions and advertise for positions where you have identified people. All I'm asking is to do it in the Journals where you would do it to satisfy the law. For example in this case it would be the MRS Bulletin, Microscopy Today, Physics Today, etc. The law doesn't require you to do it here. Just don't waste people's time.
This is totally my opinion and it comes from times when I was looking for positions where I was qualified but locked out because of this type of stuff. Also in my opinion, this type of thing is not in the spirit of a post-doc. A post-doc should be used to further broaden a recent Ph.D.'s breadth in the field, not to focus it further in an area where he/she has already done concentrated work.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
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The Center for Advanced Research and Technology (CART) at the University of North Texas (UNT) has immediate openings for two postdoctoral fellows specializing in the area of advanced characterization of metallic and hybrid materials. Applicants must have a strong commitment to research, and exhibit the potential to sustain a cutting-edge research program involving nanoscale characterization of interfaces and other microstructural features in the aforementioned materials systems. Specifically the applicant must have demonstrated experience in the use of ALL the following advanced characterization techniques:
1. Three-dimensional atom probe (3DAP) tomography including experience in analysis and interpretation of results. 2. Analytical and high-resolution transmission electron microscopy (TEM). 3. Preparation of site-specific samples for 3DAP and TEM using focused ion beam (FIB) milling techniques, such as the use of a dual-beam FIB. 4. Scanning electron microscopy (SEM) including electron backscatter diffraction (EBSD) based orientation imaging microscopy (OIM).
In addition, the applicants are required to have demonstrated research experience in the field of microstructural evolution in nickel base alloys or titanium base alloys or hybrid/composite materials. The required qualifications for all applicants include a Ph.D. in materials science and engineering.
The positions are available for one year with a possible extension based upon mutual agreement and the availability of funding.
UNT, the fourth largest university in Texas, is located in Denton, Texas 35 miles north of the Dallas/Fort Worth metropolitan area. There are approximately 34,000 students registered in 93 bachelors, 111 masters and 50 doctoral degree programs. This is an area of more than six million people, with significant economic growth, numerous industrial establishments, and excellent school districts. This area and the university provide excellent cultural and educational opportunities as well as exceptional employment opportunities for spouses. Applicants should submit a curriculum vitae and the names of at least three references by email (single PDF file) to Dr. Rajarshi Banerjee (Banerjee-at-egw.unt.edu) or Dr. J. D. Luttmer (Luttmer-at-unt.edu). To receive full consideration applications must be received by January 15, 2008.
UNT is an Affirmative Action/Equal Opportunity Employer.
==============================Original Headers============================== 7, 23 -- From drd0036-at-unt.edu Mon Nov 19 15:25:40 2007 7, 23 -- Received: from ironport1.nscmail.private.unt.edu (mailhost.unt.edu [129.120.188.67]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAJLPdLn028529 7, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Nov 2007 15:25:39 -0600 7, 23 -- X-ExtLoopCount1: 1 7, 23 -- X-IronPort-AV: E=Sophos;i="4.21,438,1188795600"; 7, 23 -- d="scan'208";a="3581966" 7, 23 -- Received: from mtsc-d2569gd1.mtsc.eng.unt.edu (HELO mtscD2569GD1) ([129.120.21.110]) 7, 23 -- by ironport1.nscmail.private.unt.edu with ESMTP; 19 Nov 2007 15:25:39 -0600 7, 23 -- From: "Dave Diercks" {drd0036-at-unt.edu} 7, 23 -- To: {Microscopy-at-microscopy.com} 7, 23 -- Subject: Postdoctoral positions at the University of North Texas 7, 23 -- Date: Mon, 19 Nov 2007 15:25:39 -0600 7, 23 -- Message-ID: {003a01c82af2$bb86e050$3294a0f0$-at-edu} 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="US-ASCII" 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- X-Mailer: Microsoft Office Outlook 12.0 7, 23 -- thread-index: Acgq8rk3PR9/1Ms5SSWPetQ/5nHNRA== 7, 23 -- Content-Language: en-us 7, 23 -- x-cr-hashedpuzzle: AFpE ALIt AOBd AQ31 AWwd AqM5 BWff DZor Dqqw FpSR F2ES F2q9 GYPl HF4B Ktai Kz05;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=; Sosha1_v1;7;{B94528E9-CADC-4D14-987D-48995FBAE115};ZAByAGQAMAAwADMANgBAAHUAb gB0AC4AZQBkAHUA;Mon, 19 Nov 2007 21:25:35 GMT;UABvAHMAdABkAG8AYwB0AG8AcgBhAGwAIABwAG8AcwBpAHQAaQBvAG4AcwAgAGEAdAAgAHQA aABlACAAVQBuAGkAdgBlAHIAcwBpAHQAeQAgAG8AZgAgAE4AbwByAHQAaAAgAFQAZQB4AGEAcwA= 7, 23 -- x-cr-puzzleid: {B94528E9-CADC-4D14-987D-48995FBAE115} ==============================End of - Headers==============================
==============================Original Headers============================== 16, 21 -- From walck-at-southbaytech.com Mon Nov 19 17:35:46 2007 16, 21 -- Received: from flpi185.prodigy.net (flpi185.sbcis.sbc.com [207.115.20.187]) 16, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAJNZjju023370 16, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Nov 2007 17:35:46 -0600 16, 21 -- X-ORBL: [64.169.217.123] 16, 21 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) 16, 21 -- by flpi185.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id lAJNZi8L028111 16, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Nov 2007 15:35:44 -0800 16, 21 -- From: "Scott Walck" {walck-at-southbaytech.com} 16, 21 -- To: {Microscopy-at-microscopy.com} 16, 21 -- Subject: RE: [Microscopy] Postdoctoral positions at the University of North Texas 16, 21 -- Date: Mon, 19 Nov 2007 15:35:44 -0800 16, 21 -- Message-ID: {001101c82b04$e82a29c0$7801a8c0-at-dynamicbl8uno3} 16, 21 -- MIME-Version: 1.0 16, 21 -- Content-Type: text/plain; 16, 21 -- charset="us-ascii" 16, 21 -- Content-Transfer-Encoding: 7bit 16, 21 -- X-Mailer: Microsoft Office Outlook 11 16, 21 -- In-Reply-To: {200711192129.lAJLTilO001518-at-ns.microscopy.com} 16, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 16, 21 -- Thread-Index: Acgq807yPUv3fE6PSt+vVgUPhsdk3QADdfTw ==============================End of - Headers==============================
Are you sure the name plate said "Dr. A. Steere"? Could it possibly be "Dr R. Steere"? There was a rather eminent biologist by the name of Russell Steere who pioneered a lot of microscopy techniques in the 1950s to whom it could have belonged. He lived the later part of his life in Baltimore I believe.
Just curious. Sounds like a great find!
Peter Ingram
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==============================Original Headers============================== 9, 21 -- From p.ingram-at-voice.cellbio.duke.edu Mon Nov 19 17:59:37 2007 9, 21 -- Received: from smtp.duke.edu (smtp-04.oit.duke.edu [152.3.174.85]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAJNxbBx003675 9, 21 -- for {microscopy-at-microscopy.com} ; Mon, 19 Nov 2007 17:59:37 -0600 9, 21 -- Received: from smtp.duke.edu (localhost.localdomain [127.0.0.1]) 9, 21 -- by localhost (Postfix) with SMTP id F38DA358F93; 9, 21 -- Mon, 19 Nov 2007 18:59:35 -0500 (EST) 9, 21 -- Received: from [152.3.54.111] (dhcp111.fel.duke.edu [152.3.54.111]) 9, 21 -- by smtp.duke.edu (Postfix) with ESMTP id 9C2FF358B51; 9, 21 -- Mon, 19 Nov 2007 18:59:35 -0500 (EST) 9, 21 -- Mime-Version: 1.0 9, 21 -- Message-Id: {a06002007c367d0b0ac57-at-[152.3.54.111]} 9, 21 -- In-Reply-To: {200711132013.lADKDGrD003491-at-ns.microscopy.com} 9, 21 -- References: {200711132013.lADKDGrD003491-at-ns.microscopy.com} 9, 21 -- Date: Mon, 19 Nov 2007 18:59:32 -0500 9, 21 -- To: randerson20-at-tampabay.rr.com 9, 21 -- From: Peter Ingram {p.ingram-at-voice.cellbio.duke.edu} 9, 21 -- Subject: Re: [Microscopy] Antique Microscope 9, 21 -- Cc: microscopy-at-microscopy.com 9, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 9, 21 -- X-PMX-Version: 5.3.3.310218, Antispam-Engine: 2.5.2.313940, Antispam-Data: 2007.11.19.153401 ==============================End of - Headers==============================
Our lab has been using ImageJ with the plug-in MosaicJ. MosaicJ is a semi-automated method; you give the program an initial guess by placing images in rough alignment, then let the program automatically translate (and rotate, if necessary) the images to assemble the final mosaic. Output quality is very high. All ImageJ types are supported for input.
On a dual-core 3GHz Pentium 4 with 4 GB RAM it takes about 10 minutes to align 10 - 15 individual 1392 x 1040 pixel RGB TIFFs.
MosaicJ has an excellent help page:
http://bigwww.epfl.ch/thevenaz/mosaicj/
I've written a few notes on installation of MosaicJ:
-- Marc Takeno, Ph.D. Research Scientist Department of Bioengineering N330B Foege (206) 543-4290 takenomm-at-u.washington.edu
On Fri, 16 Nov 2007 cookrn-at-muohio.edu wrote:
} I have also heard that ImageJ might also be decent tool to use with the TrakEM2 plugin. On average, I have approximately 20-40 800x600 pixel uncompressed TIFF images being stitched in a grid fashion, which make up the entire image of the section. My question is whether you are familiar with these tools and their abilities. Are you aware if they could perform such a task? Do you have any suggest! } ions as far as where to look for information on this topic? Any help, of course, would be greatly appreciated. Best regards, } } Ryan Cook } } ---------------------------------------------------------------------------
==============================Original Headers============================== 13, 24 -- From takenomm-at-u.washington.edu Mon Nov 19 18:12:17 2007 13, 24 -- Received: from mxout2.cac.washington.edu (mxout2.cac.washington.edu [140.142.33.4]) 13, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAK0CGoW028140 13, 24 -- for {microscopy-at-microscopy.com} ; Mon, 19 Nov 2007 18:12:17 -0600 13, 24 -- Received: from hymn33.u.washington.edu (hymn33.u.washington.edu [140.142.13.173]) 13, 24 -- by mxout2.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id lAK0CGG7025746 13, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 13, 24 -- Mon, 19 Nov 2007 16:12:16 -0800 13, 24 -- Received: from localhost (localhost [127.0.0.1]) 13, 24 -- by hymn33.u.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id lAK0CGb7003824; 13, 24 -- Mon, 19 Nov 2007 16:12:16 -0800 13, 24 -- X-Auth-Received: from [128.208.18.130] by hymn33.u.washington.edu via HTTP; Mon, 19 Nov 2007 16:12:16 PST 13, 24 -- Date: Mon, 19 Nov 2007 16:12:16 -0800 (PST) 13, 24 -- From: Marc M Takeno {takenomm-at-u.washington.edu} 13, 24 -- To: microscopy-at-microscopy.com 13, 24 -- cc: cookrn-at-muohio.edu 13, 24 -- Subject: Re: [Microscopy] AskAMicroscopist: Stitching High-Resolution Microscope 13, 24 -- Images 13, 24 -- In-Reply-To: {200711162259.lAGMxYFR022428-at-ns.microscopy.com} 13, 24 -- Message-ID: {Pine.LNX.4.43.0711191612160.13971-at-hymn33.u.washington.edu} 13, 24 -- MIME-Version: 1.0 13, 24 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 13, 24 -- X-PMX-Version: 5.3.3.310218, Antispam-Engine: 2.5.2.313940, Antispam-Data: 2007.11.19.154826 13, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='SUPERLONG_LINE 0.05, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
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We are considering purchasing a multi-photon microscope in the near future and would like to hear from anyone who has gone down this path before. Information about available commercial systems, their performance, ease of maintenance, quality of service, etc. are some of the things we are hoping to learn.
I have used the previous Zeiss 510 microscope and liked it very much. It is user-friendly and powerful. I don't know the technical details of the Leica but in the Zeiss the different wavelengthes are separated not only by filters but also in time, so no read-through is possible. I must say this gives a welcome level of confidence and comfort. I did a lot of triple staining with great results.
No interest blabla....
Regards, Stephane
----- Original Message ---- X-from: "christopher.hayden-at-novartis.com" {christopher.hayden-at-novartis.com} To: nizets2-at-yahoo.com Sent: Monday, November 19, 2007 7:28:16 PM
Good Day, All:
We're in the fortunate position of looking around at acquiring a confocal microscope sometime in 2008. Before we start hearing the pitches from the manufacturers, we'd like to hear from the "real" world. Specifically, what you have and what you think of it (ease-of-use, upgrades, level of control, service and support, etc.). After having a look around, it seems like the two contenders for us are the Zeiss 510 META NLO and the Leica TCS SP5. Flexibility is key for us, as it's going to have a fairly broad set of uses as both a research tool and as a development tool. Cost isn't critical at this point; we're basically being told to "get what we need". While we have people with confocal experience, each is
a little biased that the system they know is the best one.
Thanks much! -Chris
==============================Original Headers============================== 5, 29 -- From christopher.hayden-at-novartis.com Mon Nov 19 12:23:16 2007 5, 29 -- Received: from mail194.messagelabs.com (mail194.messagelabs.com [85.158.140.211]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAJINGAI008522 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 12:23:16 -0600 5, 29 -- X-VirusChecked: Checked 5, 29 -- X-Env-Sender: christopher.hayden-at-novartis.com 5, 29 -- X-Msg-Ref: server-10.tower-194.messagelabs.com!1195496595!1972703!1 5, 29 -- X-StarScan-Version: 5.5.12.14.2; banners=-,-,- 5, 29 -- X-Originating-IP: [160.62.1.174] 5, 29 -- Received: (qmail 15936 invoked from network); 19 Nov 2007 18:23:15 -0000 5, 29 -- Received: from ch2ssaenov01.novartis.com (HELO ch2ssaenov01.novartis.com) (160.62.1.174) 5, 29 -- by server-10.tower-194.messagelabs.com with AES256-SHA encrypted SMTP; 19 Nov 2007 18:23:15 -0000 5, 29 -- Received: from ch1ssainov01.novartis.net (ch1ssainov01s [192.37.2.173]) 5, 29 -- by ch2ssaenov01.novartis.com (8.13.8/8.13.8) with ESMTP id lAJINE00017372 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 19:23:14 +0100 5, 29 -- Received: from phchbs-s3140.EU.novartis.net (phchbs-s3140.eu.novartis.net [192.37.31.248]) 5, 29 -- by ch1ssainov01.novartis.net (8.13.8/8.13.8) with ESMTP id lAJINE7X016212 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 19:23:14 +0100 5, 29 -- To: Microscopy-at-Microscopy.Com 5, 29 -- Subject: LM - Confocal Opinions 5, 29 -- MIME-Version: 1.0 5, 29 -- X-Mailer: Lotus Notes Release 7.0.1 HF517 January 12, 2007 5, 29 -- Message-ID: {OFC44BD91C.2AD7BFB4-ON85257398.0064ECC4-85257398.006500CF-at-ah.novartis.com} 5, 29 -- From: christopher.hayden-at-novartis.com 5, 29 -- Date: Mon, 19 Nov 2007 13:23:13 -0500 5, 29 -- X-MIMETrack: Serialize by Router on CHBSSPH0/PH/Novartis(Release 7.0.2FP2 HF144|July 27, 2007) at 5, 29 -- 19.11.2007 19:23:14, 5, 29 -- Serialize complete at 19.11.2007 19:23:14 5, 29 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
____________________________________________________________________________________ Be a better pen pal. Text or chat with friends inside Yahoo! Mail. See how. http://overview.mail.yahoo.com/
==============================Original Headers============================== 19, 20 -- From nizets2-at-yahoo.com Tue Nov 20 08:39:39 2007 19, 20 -- Received: from web37414.mail.mud.yahoo.com (web37414.mail.mud.yahoo.com [209.191.91.146]) 19, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lAKEdc98014460 19, 20 -- for {microscopy-at-microscopy.com} ; Tue, 20 Nov 2007 08:39:38 -0600 19, 20 -- Received: (qmail 3622 invoked by uid 60001); 20 Nov 2007 14:39:38 -0000 19, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 19, 20 -- s=s1024; d=yahoo.com; 19, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 19, 20 -- b=QvMKdQxtyTgYygoA+/WvTMoT6VrXnErFZYqAO8HBdjZ75Bue5UJBJbgQPJoe1IYxXgEoma8MAFZHlbikrCvN8T1cVFrMKOKxQdtmjmWpBbjdyQw4r7t8pjB+ZDGttvxwrE1JWZc92VlBK8YAbSzdfqKtnYK4XOUDB2MTaorddgc=; 19, 20 -- X-YMail-OSG: _M57H5YVM1kRdmMtoug4Z4BZZz86tUrbpxFAdpWyf5STGplD138_1agXHOztVYU9dxKkhrHX458qgS.taSMs3DSY4v7.MCqALlncXJ2nPgY12t_n2btruL9UjvzbomdQK2sTPqrrvgG.C_HY_EyOavrLxiafpuKm6R4yH_jGKBupkv4_3lPm7cs- 19, 20 -- Received: from [80.122.101.100] by web37414.mail.mud.yahoo.com via HTTP; Tue, 20 Nov 2007 06:39:38 PST 19, 20 -- X-Mailer: YahooMailRC/818.27 YahooMailWebService/0.7.157 19, 20 -- Date: Tue, 20 Nov 2007 06:39:38 -0800 (PST) 19, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 19, 20 -- Subject: Re: [Microscopy] LM - Confocal Opinions 19, 20 -- To: christopher.hayden-at-novartis.com 19, 20 -- Cc: microscopy-at-microscopy.com 19, 20 -- MIME-Version: 1.0 19, 20 -- Content-Type: text/plain; charset=us-ascii 19, 20 -- Message-ID: {405818.2251.qm-at-web37414.mail.mud.yahoo.com} ==============================End of - Headers==============================
We are struggling to visualize the flagella of bacteria by TEM. We do very ordinary negative staining with 2% uranyl acetate. We tried with and without fixation, and even tried ethanol dehydration but the main problem is not the staining in itself, it is that almost no bacteria stay on the carbon film! We tried to incubate the bacteria with the grids in the medium (2 days), we tried to glue the carbon film with the glue from dissolved 3M tape, to no avail. Yes, the glue fixed a lot of trash on the grid but not the bacteria! I am wondering if the bacteria don't stick or if they explode due to the vacuum of the TEM column (LaB6 200 kV TEM). Sometimes we got several bacteria and could see something, but most of the time the flagella were broken and were not attached to the bacteria anymore. We tried folmvar coated grids but it seems even worse. We cannot glow discharge the carbon grids but we leave them for 30 min under a UV light at 254 nm (this is a machine used to sterilize material for cell culture).
Any comment welcome and appreciated.
I am now considering shadow casting with our basic coating unit (for SEM) so any comment in this direction is welcome too. We have only carbon, gold and silver target and low vaccum. Observation of the flagella by SEM is not an option, it is a basic W analytical SEM, the resolution is not sufficient to resolve 20 nm wide structures.
Regards, Stephane
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==============================Original Headers============================== 7, 19 -- From nizets2-at-yahoo.com Tue Nov 20 08:49:58 2007 7, 19 -- Received: from web37408.mail.mud.yahoo.com (web37408.mail.mud.yahoo.com [209.191.91.140]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lAKEnwNX026219 7, 19 -- for {microscopy-at-microscopy.com} ; Tue, 20 Nov 2007 08:49:58 -0600 7, 19 -- Received: (qmail 24993 invoked by uid 60001); 20 Nov 2007 14:49:58 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 7, 19 -- b=MowB+KfckskQ+cI8oP239psoOl0BBRsHFGzNmPm/8WzHGJKT1aqb+NvcLayc7eWdZJ6YW5tDyatGNOxItvRTTn2/sUcnQzKjZqK2IonbsOSXyAlq1j0LDuvLDAhcNTCZY7BOSpryuRxFfiWhEuxy/oaB8EtERnDEIWf5kJbZnM0=; 7, 19 -- X-YMail-OSG: t_Xw6cIVM1me0pw8ISEE6sPzf_KxrMn0NREHYpLl7Q0_6CZcBEa1chsq5kp0YX2iJZA55vMQIsLni.QXj065oLYccxhDHFV4PF0pXU6biLKLxO7QdY0- 7, 19 -- Received: from [80.122.101.100] by web37408.mail.mud.yahoo.com via HTTP; Tue, 20 Nov 2007 06:49:58 PST 7, 19 -- X-Mailer: YahooMailRC/818.27 YahooMailWebService/0.7.157 7, 19 -- Date: Tue, 20 Nov 2007 06:49:58 -0800 (PST) 7, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 19 -- Subject: Visualization of bacteria flagella: troubleshooter needed 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=us-ascii 7, 19 -- Message-ID: {278093.24910.qm-at-web37408.mail.mud.yahoo.com} ==============================End of - Headers==============================
Commercial response: Image-Pro from Media Cybernetics stitches images quite well, using a Fourier cross-correlation calculation. I've stitched } 500 images together in a grid, with output sizes on the order of 10Kx10K pixels.
Seems to work very well, but I am biased, having written that module!
Kevin Ryan Media Cybernetics, Inc. www.mediacy.com
} -----Original Message----- } From: cookrn-at-muohio.edu [mailto:cookrn-at-muohio.edu] } Sent: Friday, November 16, 2007 5:59 PM } } Question: I am working on an independent study project to analyze the } differences in lignin quantities between the roots and the stems cut from } the four cardinal directions from different tree genera. I have taken } high resolution micrographs of sections that I cut and stained, but am } having trouble finding an adequate solution to stitching the photos } together. I have toyed with Hugin and PTAssembler, which both utilize the } open-source Panorama Tools library, Autopano, and Enblend. Yet, I am } either not using these tools correctly, or they are not adequate for what } I want to do. I have also heard that ImageJ might also be decent tool to } use with the TrakEM2 plugin. On average, I have approximately 20-40 } 800x600 pixel uncompressed TIFF images being stitched in a grid fashion, } which make up the entire image of the section. My question is whether you } are familiar with these tools and their abilities. Are you aware if they } could perform such a task? Do you have any suggest! } ions as far as where to look for information on this topic? Any help, of } course, would be greatly appreciated. Best regards, } } Ryan Cook
==============================Original Headers============================== 6, 24 -- From kevin-at-mediacy.com Tue Nov 20 08:52:29 2007 6, 24 -- Received: from outbound.exchangemessage.net (outbound.exchangemessage.net [128.241.127.69]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAKEqSlF032275 6, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Nov 2007 08:52:28 -0600 6, 24 -- Received: from MCLUS26006MAP.exmsg.net ([128.241.126.171]) by outbound.exchangemessage.net with Microsoft SMTPSVC(6.0.3790.1830); 6, 24 -- Tue, 20 Nov 2007 09:52:27 -0500 6, 24 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 6, 24 -- Content-class: urn:content-classes:message 6, 24 -- MIME-Version: 1.0 6, 24 -- Content-Type: text/plain; 6, 24 -- charset="us-ascii" 6, 24 -- Subject: [Commercial] RE: [Microscopy] AskAMicroscopist: Stitching High-Resolution Microscope Images 6, 24 -- Date: Tue, 20 Nov 2007 09:54:26 -0500 6, 24 -- Message-ID: {254DA59D1DDEAB47AAC2EFC15FE7A8A101F938DB-at-MCLUS26006MAP.exmsg.net} 6, 24 -- In-Reply-To: {200711162258.lAGMwqa0020668-at-ns.microscopy.com} 6, 24 -- X-MS-Has-Attach: 6, 24 -- X-MS-TNEF-Correlator: 6, 24 -- Thread-topic: [Commercial] RE: [Microscopy] AskAMicroscopist: Stitching High-Resolution Microscope Images 6, 24 -- Thread-index: AcgopEWnLzlGN033RuezsH/W1inj9QC4Ic6w 6, 24 -- From: "Kevin Ryan" {kevin-at-mediacy.com} 6, 24 -- To: {cookrn-at-muohio.edu} , {Microscopy-at-microscopy.com} 6, 24 -- X-OriginalArrivalTime: 20 Nov 2007 14:52:27.0006 (UTC) FILETIME=[F79DF1E0:01C82B84] 6, 24 -- Content-Transfer-Encoding: 8bit 6, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lAKEqSlF032275 ==============================End of - Headers==============================
This is not elegant - try drying a whole drop of (maybe diluted or maybe fixed and rinsed [although I have read centrifugation can be detaching for flagellae]) sample on the grid - they cannot go anywhere. Then stain. If this does not work you could try adding stain to the sample drop.
Dave
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: 20 November 2007 14:53 To: David Patton
Hi to all!
We are struggling to visualize the flagella of bacteria by TEM. We do very ordinary negative staining with 2% uranyl acetate. We tried with and without fixation, and even tried ethanol dehydration but the main problem is not the staining in itself, it is that almost no bacteria stay on the carbon film! We tried to incubate the bacteria with the grids in the medium (2 days), we tried to glue the carbon film with the glue from dissolved 3M tape, to no avail. Yes, the glue fixed a lot of trash on the grid but not the bacteria! I am wondering if the bacteria don't stick or if they explode due to the vacuum of the TEM column (LaB6 200 kV TEM). Sometimes we got several bacteria and could see something, but most of the time the flagella were broken and were not attached to the bacteria anymore. We tried folmvar coated grids but it seems even worse. We cannot glow discharge the carbon grids but we leave them for 30 min under a UV light at 254 nm (this is a machine used to sterilize material for cell culture).
Any comment welcome and appreciated.
I am now considering shadow casting with our basic coating unit (for SEM) so any comment in this direction is welcome too. We have only carbon, gold and silver target and low vaccum. Observation of the flagella by SEM is not an option, it is a basic W analytical SEM, the resolution is not sufficient to resolve 20 nm wide structures.
Regards, Stephane
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==============================Original Headers============================== 7, 19 -- From nizets2-at-yahoo.com Tue Nov 20 08:49:58 2007 7, 19 -- Received: from web37408.mail.mud.yahoo.com (web37408.mail.mud.yahoo.com [209.191.91.140]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lAKEnwNX026219 7, 19 -- for {microscopy-at-microscopy.com} ; Tue, 20 Nov 2007 08:49:58 -0600 7, 19 -- Received: (qmail 24993 invoked by uid 60001); 20 Nov 2007 14:49:58 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Conten t-Type:Message-ID; 7, 19 -- b=MowB+KfckskQ+cI8oP239psoOl0BBRsHFGzNmPm/8WzHGJKT1aqb+NvcLayc7eWdZJ6YW5 tDyatGNOxItvRTTn2/sUcnQzKjZqK2IonbsOSXyAlq1j0LDuvLDAhcNTCZY7BOSpryuRxFfi WhEuxy/oaB8EtERnDEIWf5kJbZnM0=; 7, 19 -- X-YMail-OSG: t_Xw6cIVM1me0pw8ISEE6sPzf_KxrMn0NREHYpLl7Q0_6CZcBEa1chsq5kp0YX2iJZA55vMQ IsLni.QXj065oLYccxhDHFV4PF0pXU6biLKLxO7QdY0- 7, 19 -- Received: from [80.122.101.100] by web37408.mail.mud.yahoo.com via HTTP; Tue, 20 Nov 2007 06:49:58 PST 7, 19 -- X-Mailer: YahooMailRC/818.27 YahooMailWebService/0.7.157 7, 19 -- Date: Tue, 20 Nov 2007 06:49:58 -0800 (PST) 7, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 19 -- Subject: Visualization of bacteria flagella: troubleshooter needed 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=us-ascii 7, 19 -- Message-ID: {278093.24910.qm-at-web37408.mail.mud.yahoo.com} ==============================End of - Headers==============================
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==============================Original Headers============================== 19, 38 -- From David.Patton-at-uwe.ac.uk Tue Nov 20 09:02:20 2007 19, 38 -- Received: from mailapp04.uwe.ac.uk (mailapp04.uwe.ac.uk [164.11.132.66]) 19, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lAKF2JJF018958 19, 38 -- for {microscopy-at-microscopy.com} ; Tue, 20 Nov 2007 09:02:20 -0600 19, 38 -- Received: from (unknown [164.11.132.62]) by mailapp04.uwe.ac.uk with smtp 19, 38 -- id 07e6_8179de74_9778_11dc_ae37_00142223915c; 19, 38 -- Tue, 20 Nov 2007 14:54:40 +0000 19, 38 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 19, 38 -- (egen-uwe01.uwe.ac.uk [164.11.249.121]) 19, 38 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 19, 38 -- 2005)) with SMTP id {0JRT0033D8FNVI-at-mta02.uwe.ac.uk} for 19, 38 -- microscopy-at-microscopy.com; Tue, 20 Nov 2007 15:02:12 +0000 (GMT) 19, 38 -- Date: Tue, 20 Nov 2007 15:02:00 +0000 19, 38 -- From: David Patton {David.Patton-at-uwe.ac.uk} 19, 38 -- Subject: RE: [Microscopy] Visualization of bacteria flagella: troubleshooter 19, 38 -- needed 19, 38 -- In-reply-to: {200711201453.lAKEr3eY001598-at-ns.microscopy.com} 19, 38 -- To: nizets2-at-yahoo.com 19, 38 -- Cc: microscopy-at-microscopy.com 19, 38 -- Message-id: {F247F674896BE243AD8263C5280E2BDB0334BDAB-at-egen-uwe01} 19, 38 -- MIME-version: 1.0 19, 38 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 19, 38 -- Content-type: text/plain; charset=us-ascii 19, 38 -- Content-class: urn:content-classes:message 19, 38 -- Thread-topic: [Microscopy] Visualization of bacteria flagella: troubleshooter 19, 38 -- needed 19, 38 -- Thread-index: AcgrhQ+jSZNuwk+ITfaOgPXL8tywBAAAEOPQ 19, 38 -- X-MS-Has-Attach: 19, 38 -- X-MS-TNEF-Correlator: 19, 38 -- References: {200711201453.lAKEr3eY001598-at-ns.microscopy.com} 19, 38 -- X-NAIMIME-Disclaimer: 1 19, 38 -- X-NAIMIME-Modified: 1 19, 38 -- X-NAI-Spam-Level: 19, 38 -- X-NAI-Spam-Score: 0.5 19, 38 -- X-NAI-Spam-Rules: 3 Rules triggered 19, 38 -- MFE_BAD_ST_1=1, BAYES_20=-0.5, HAS_X_HELO=0 19, 38 -- Content-Transfer-Encoding: 8bit 19, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lAKF2JJF018958 ==============================End of - Headers==============================
You do not mention the details of how you add the bacteria to the grid. Perhaps you are wicking too much of the bacterial sample off the grid?
I had this problem years ago. The investigator claimed there were millions of billions of bacteria in the samples, but we could not find any on the grids. It turned out I was wicking them all off the grid, as I would blot off most of the sample like I would do with a virus sample. But the bacteria are large enough to get swept up into the stream as the sample is wicked up with a filter paper.
After adding a drop of sample to the grid, wick off the excess but leave a film of sample behind, so that looking across the grid you see a gentle rise of sample. Then air dry, may take 10-20 minutes, then add your stain and wick that off as usual. One problem with this is that if there is lots of culture media, buffer, etc, in the sample, the cells may be buried or contaminated when all that dries down, thought some may be removed by the staining step.
Another method, which results in a much cleaner background, is to coat the Formvar coated grids with an "adhesive" agent like poly-l-lysine or poly-ethyleneimine. I can send details if you want them. Then you place a grid filmed side down onto a drop of the bacterial sample for 5-10 minutes. Bacteria will stick to the grid. Then transfer to drop of fixative if desired, or to rinse buffer, then to drop of negative stain, then blot that dry. This method usually yields a nice even distribution of bacteria across the grid. The flagella may be less likely to separate from the cells as they are "glued" down to the Formvar film along with the parent cell body.
Hope this helps., but let us know what works for you.
Gib -- Gilbert (Gib) Ahlstrand, Electron Microscopist, Imaging Center, University of Minnesota 123 Snyder Hall St. Paul, MN 55108 http://www.cbs.umn.edu/ic
nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi to all! } } We are struggling to visualize the flagella of bacteria by TEM. We do very ordinary negative staining with 2% uranyl acetate. } We tried with and without fixation, and even tried ethanol dehydration but the main problem is not the staining in itself, it is that almost no bacteria stay on the carbon film! We tried to incubate the bacteria with the grids in the medium (2 days), we tried to glue the carbon film with the glue from dissolved 3M tape, to no avail. Yes, the glue fixed a lot of trash on the grid but not the bacteria! } I am wondering if the bacteria don't stick or if they explode due to the vacuum of the TEM column (LaB6 200 kV TEM). } Sometimes we got several bacteria and could see something, but most of the time the flagella were broken and were not attached to the bacteria anymore. } We tried folmvar coated grids but it seems even worse. } We cannot glow discharge the carbon grids but we leave them for 30 min under a UV light at 254 nm (this is a machine used to sterilize material for cell culture). } } Any comment welcome and appreciated. } } I am now considering shadow casting with our basic coating unit (for SEM) so any comment in this direction is welcome too. We have only carbon, gold and silver target and low vaccum. Observation of the flagella by SEM is not an option, it is a basic W analytical SEM, the resolution is not sufficient to resolve 20 nm wide structures. } } Regards, } Stephane
==============================Original Headers============================== 8, 22 -- From ahlst007-at-umn.edu Tue Nov 20 10:09:11 2007 8, 22 -- Received: from mta-a2.tc.umn.edu (mta-a2.tc.umn.edu [134.84.119.206]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAKG9991000768 8, 22 -- for {Microscopy-at-Microscopy.com} ; Tue, 20 Nov 2007 10:09:10 -0600 8, 22 -- Received: from [160.94.39.28] (x160-94-39-28.cbs.umn.edu [160.94.39.28]) 8, 22 -- by mta-a2.tc.umn.edu (UMN smtpd) with ESMTP 8, 22 -- for {Microscopy-at-Microscopy.com} ; Tue, 20 Nov 2007 10:09:03 -0600 (CST) 8, 22 -- X-Umn-Remote-Mta: [N] x160-94-39-28.cbs.umn.edu [160.94.39.28] #+LO+TS+AU+HN 8, 22 -- Message-ID: {47430653.9060501-at-umn.edu} 8, 22 -- Date: Tue, 20 Nov 2007 10:07:47 -0600 8, 22 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 8, 22 -- Reply-To: ahlst007-at-umn.edu 8, 22 -- Organization: Imaging Center UM 8, 22 -- User-Agent: Thunderbird 1.5.0.13 (Macintosh/20070809) 8, 22 -- MIME-Version: 1.0 8, 22 -- To: Microscopy-at-Microscopy.com 8, 22 -- Subject: Re: [Microscopy] Visualization of bacteria flagella: troubleshooter 8, 22 -- needed 8, 22 -- References: {200711201452.lAKEqM8S032140-at-ns.microscopy.com} 8, 22 -- In-Reply-To: {200711201452.lAKEqM8S032140-at-ns.microscopy.com} 8, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
On the S-4500 SEM the is a Button "Low Mag", With that button pressed the maximum magnification is around x1000. I don't know if it is the same with the S-3000N.
Patrick
----- Message d'origine ---- De : "malcolm.haswell-at-sunderland.ac.uk" {malcolm.haswell-at-sunderland.ac.uk} À : weis183-at-yahoo.fr Envoyé le : Vendredi, 16 Novembre 2007, 18h02mn 34s Objet : [Microscopy] Re: viaWWW: Max magnification problems Hitachi S-3000N
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Michael
I have an S-3000N so I thought I would check my values for magnifications.
I assume that you mean that when you turn the magnification control that the maximum value that you can reach is either 750x or 1000x after which more turning in the same direction results in the beeping sound when you've turned the magn digital encoder knob too far. Furthermore I also assume that the magnification readout at the top of the screen and on the black data bar have the same value and the scale bar doesn't change once you've reached the maximum.
If all of this is true then maybe my readings will help: at 30kv WD 3mm magn range is x100 to x300k; WD 60mm magn range x15 to x60k at 20kv WD 3mm magn range is x90 to x300k; WD 60mm magn range x15 to x60k at 5kv WD 3mm magn range is x60 to x200k; WD 60mm magn range x15 to x45k at0.3kv WD 3mm magn range is x50 to x50k; WD 60mm magn range x15 to x10k
so the lowest magn is between x15 and x100 depending on WD and kv and the highest magn is between x10k and x300k depending on WD and kv
What worries me a little is that you quote a value of 15k as a previous best where I would have to be operating at 1kv and maximum WD of 60mm to get such a low value.
If you are simply talking about poor resolution rather than max magnification values then ignore this.
Sorry if I've got the wrong end of the stick but it might be worth you checking a range of min max magnifications for different kvs and WDs.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: mcinerne-at-rose-hulman.edu
My S-3000N does not any button like this. I personally think that the remark about relay problems may be going in the right direction. I have had some troubles on occasion with them not cycling as desired.
Richard Shalvoy Arch Chemicals, Inc. 350 Knotter Drive Cheshire, CT 06410 (203) 271-4394 rbshalvoy-at-archchemicals.com
-----Original Message----- X-from: weis183-at-yahoo.fr [mailto:weis183-at-yahoo.fr] Sent: Tuesday, November 20, 2007 11:41 AM To: Shalvoy, Richard B **CHES
On the S-4500 SEM the is a Button "Low Mag", With that button pressed the maximum magnification is around x1000. I don't know if it is the same with the S-3000N.
Patrick
----- Message d'origine ---- De : "malcolm.haswell-at-sunderland.ac.uk" {malcolm.haswell-at-sunderland.ac.uk} À : weis183-at-yahoo.fr Envoyé le : Vendredi, 16 Novembre 2007, 18h02mn 34s Objet : [Microscopy] Re: viaWWW: Max magnification problems Hitachi S-3000N
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
Michael
I have an S-3000N so I thought I would check my values for magnifications.
I assume that you mean that when you turn the magnification control that the maximum value that you can reach is either 750x or 1000x after which more turning in the same direction results in the beeping sound when you've turned the magn digital encoder knob too far. Furthermore I also assume that the magnification readout at the top of the screen and on the black data bar have the same value and the scale bar doesn't change once you've reached the maximum.
If all of this is true then maybe my readings will help: at 30kv WD 3mm magn range is x100 to x300k; WD 60mm magn range x15 to x60k at 20kv WD 3mm magn range is x90 to x300k; WD 60mm magn range x15 to x60k at 5kv WD 3mm magn range is x60 to x200k; WD 60mm magn range x15 to x45k at0.3kv WD 3mm magn range is x50 to x50k; WD 60mm magn range x15 to x10k
so the lowest magn is between x15 and x100 depending on WD and kv and the highest magn is between x10k and x300k depending on WD and kv
What worries me a little is that you quote a value of 15k as a previous best where I would have to be operating at 1kv and maximum WD of 60mm to get such a low value.
If you are simply talking about poor resolution rather than max magnification values then ignore this.
Sorry if I've got the wrong end of the stick but it might be worth you checking a range of min max magnifications for different kvs and WDs.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: mcinerne-at-rose-hulman.edu
Commercial solution:
Hi Ryan,
SIMAGIS Smart Imaging Spreadsheet has an advanced image stitching tool that may used in automatic or manual mode to assemble very large image sets.
More information is available at: http://www.smartimtech.com/analysis/research_new.htm#stitching
Z-axis image stacking is also available in the SIMAGIS workbench.
SIMAGIS spreadsheets are advancing the usefulness of image data in the same way that Excel spreadsheets improved handling of numerical data. You don't need programming skills to achieve meaningful results.
Hope this helps.
Regards,
Ira Bleiweiss Smart Imaging Technologies 713.589.3216 direct 877.280.1100 ext.1004 Toll Free (US) ira-at-simagis.us www.smartimtech.com
Advanced Software Solutions for Science and Industry
------------------------------------------------------------------ Email: cookrn-at-muohio.edu Name: Ryan Cook
Question: I am working on an independent study project to analyze the differences in lignin quantities between the roots and the stems cut from the four cardinal directions from different tree genera. I have taken high resolution micrographs of sections that I cut and stained, but am having trouble finding an adequate solution to stitching the photos together. I have toyed with Hugin and PTAssembler, which both utilize the open-source Panorama Tools library, Autopano, and Enblend. Yet, I am either not using these tools correctly, or they are not adequate for what I want to do. I have also heard that ImageJ might also be decent tool to use with the TrakEM2 plugin. On average, I have approximately 20-40 800x600 pixel uncompressed TIFF images being stitched in a grid fashion, which make up the entire image of the section. My question is whether you are familiar with these tools and their abilities. Are you aware if they could perform such a task? Do you have any suggest! ions as far as where to look for information on this topic? Any help, of course, would be greatly appreciated. Best regards,
==============================Original Headers============================== 10, 13 -- From zaluzec-at-ultra5.microscopy.com Fri Nov 16 16:47:36 2007 10, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 10, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAGMlZqF032140 10, 13 -- for {microscopy-at-microscopy.com} ; Fri, 16 Nov 2007 16:47:36 -0600 10, 13 -- Mime-Version: 1.0 10, 13 -- Message-Id: {p06240801c363ce71bdf6-at-[206.69.208.22]} 10, 13 -- Date: Fri, 16 Nov 2007 16:47:34 -0600 10, 13 -- To: microscopy-at-microscopy.com 10, 13 -- From: cookrn-at-muohio.edu (by way of Ask-A-Microscopist) 10, 13 -- Subject: AskAMicroscopist: Stitching High-Resolution Microscope Images 10, 13 -- Content-Type: text/plain; charset="us-ascii" 10, 13 -- Content-Transfer-Encoding: 8bit 10, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lAGMlZqF032140 ==============================End of - Headers==============================
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==============================Original Headers============================== 23, 22 -- From xjsun-at-ualberta.ca Fri Nov 16 18:15:44 2007 23, 22 -- Received: from mailgw1.cancerboard.ab.ca (mx1.cancerboard.ab.ca [216.123.198.11]) 23, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAH0FhEg015729 23, 22 -- for {microscopy-at-microscopy.com} ; Fri, 16 Nov 2007 18:15:43 -0600 23, 22 -- Reply-To: {xjsun-at-ualberta.ca} 23, 22 -- From: "Xuejun Sun" {xjsun-at-ualberta.ca} 23, 22 -- To: {microscopy-at-microscopy.com} 23, 22 -- Cc: {cookrn-at-muohio.edu} 23, 22 -- References: {200711162254.lAGMs11B013609-at-ns.microscopy.com} 23, 22 -- Subject: RE: [Microscopy] AskAMicroscopist: Stitching High-Resolution Microscope Images 23, 22 -- Date: Thu, 16 Nov 2006 17:15:42 -0700 23, 22 -- Organization: UA 23, 22 -- Message-ID: {000c01c709dd$84cae3e0$29e966a4-at-ad.cancerboard.ab.ca} 23, 22 -- MIME-Version: 1.0 23, 22 -- Content-Type: text/plain; 23, 22 -- charset="iso-8859-1" 23, 22 -- Content-Transfer-Encoding: 7bit 23, 22 -- X-Mailer: Microsoft Office Outlook 11 23, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 23, 22 -- Thread-Index: Acgoo5VRYjw3ddLzQ9SZ7lteq9fO5QABfsxg 23, 22 -- In-Reply-To: {200711162254.lAGMs11B013609-at-ns.microscopy.com} 23, 22 -- X-OriginalArrivalTime: 17 Nov 2007 00:15:42.0879 (UTC) FILETIME=[FDDFEAF0:01C828AE] ==============================End of - Headers==============================
==============================Original Headers============================== 24, 23 -- From ira-at-simagis.us Tue Nov 20 15:18:41 2007 24, 23 -- Received: from smtp167.iad.emailsrvr.com (smtp167.iad.emailsrvr.com [207.97.245.167]) 24, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAKLIfhs032038 24, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Nov 2007 15:18:41 -0600 24, 23 -- Received: from relay6.relay.iad.emailsrvr.com (localhost [127.0.0.1]) 24, 23 -- by relay6.relay.iad.emailsrvr.com (SMTP Server) with ESMTP id 7F1866D833B; 24, 23 -- Tue, 20 Nov 2007 16:18:40 -0500 (EST) 24, 23 -- Received: by relay6.relay.iad.emailsrvr.com (Authenticated sender: ira-AT-simagis.us) with ESMTP id 33EB46D8734; 24, 23 -- Tue, 20 Nov 2007 16:18:40 -0500 (EST) 24, 23 -- From: "Ira Bleiweiss" {ira-at-simagis.us} 24, 23 -- To: {Microscopy-at-microscopy.com} 24, 23 -- Cc: {cookrn-at-muohio.edu} 24, 23 -- Subject: RE: AskAMicroscopist: Stitching High-Resolution Microscope Images 24, 23 -- Date: Tue, 20 Nov 2007 15:18:30 -0600 24, 23 -- Message-ID: {030901c82bba$e65bdab0$17010a0a-at-iblaptop} 24, 23 -- MIME-Version: 1.0 24, 23 -- Content-Type: text/plain; 24, 23 -- charset="us-ascii" 24, 23 -- Content-Transfer-Encoding: 7bit 24, 23 -- X-Mailer: Microsoft Office Outlook 11 24, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 24, 23 -- In-Reply-To: {200711170024.lAH0OvZ3027841-at-ns.microscopy.com} 24, 23 -- Thread-Index: AcgosElZQIidelpUQhCe9qsdQ9DWAwDCWPuQ ==============================End of - Headers==============================
Stephane, Here are some listings about negative stained bacteria posted about two years ago. I hope it helps. Larry
We have a few users study bacterial pili using negative staining with PTA (pH 6.5). We have got some pretty nice images. But often time, we could not find pili even on the positive control. When we did see pili, they were not on all bacteria in the same sample. Is this a common phenomenon? Do bacteria lose their pili easily when the external condition is not favorable? if that is the case, what should be done to minimize the loss.
Thank you in advance.
Hong Emory EM
We often have this same problem. At least in our lab, flagella and pili seem to hide when we're trying to find them and show up when we don't care if we see them or not.
As a rule, they seem to be easily detached by rough handling, including centrifuging, and maybe by the staining process itself, since we often find them isolated from the bacteria on the grid.
One group of researchers gets around this problem by growing the bacteria on carbon coated grids and staining them in situ. Check JOURNAL OF BACTERIOLOGY, Feb. 2005, p. 1173-1181 for images. For preparation details this article references Brown et al, 2001. Immunocytochemical localization of HrpA and HrpZ supports a role for the Hrp pilus in the transfer of effector proteins from Pseudomonassyringae pv. tomato across the host plant cell wall. Mol. Plant-Microbe Interact. 14:394-404. I have not yet read the last article, so I can't comment on it.
Good luck.
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
Rough handling may contribute to loss of pili, however the PTA itself is probably the culprit. Try changing the pH either up or down. Way back when, I did a study with rotavirus and various negative stains. Bottom line is that the length of time of staining, pH and concentration all contributed to the destruction (preservation) of the viral particles. If I remember correctly, the length of time was the most important factor. Also try using either uranyl acetate or ammonium molybdate as the negative stain.
Best of luck,
Ed
Edward P. Calomeni Director EM Lab - Pathology The Ohio State University M018 Starling Loving Hall 320 W. 10th Ave. Columbus, OH 43210 614-293-5580 (office) 614-293-8806 (lab) edward.calomeni-at-osumc.edu
I have been using 2% Uranyl acetate to visualize pili. I have had to play with the time of staining and rinsing in order to get a good contrast to visualize the pili on different bacterial genera. I have noticed that not all bacteria show the same distribution of pili. I have also noticed that the way you grow the bacteria also affect visualization of pili. For our bacteria I have noticed that I get far better results when I grow them up in stationary cultures without shaking them.
What I have been seeing it the bacteria behave differently when in aggregation versus solitude. This is more so owing to the several differnt kinds of pili each bacterial strain is capable of forming. If the bacteria you are working with produces different types of pili then you will see differences between the bacterial cells visualized on the same grid.
I have had the same strain of bacteria producing really small pili which were visualized only at a very high mag. Hope that was helpful regards, Vinod Graduate Student Dept Of Biology New Mexico State University
this is what we have done in the past while negatively staining Gram negative bacteria Questions:
} } } } 1. What cell density is required to obtain one layer of cells on the } } } } grid but not cells on top of each other?
2. Are there ways of increasing the bacterial affinity for the grids?
} } } } 3. Are there ways to reduce the amount of debris on the grids? } } } } 4. Can grids be incubated on top of a nitrocellulose filter without } } } } beeing damaged?
Answer: It might be challenging to achieve a monolayer of bacterial cells on the grid as every bacteria behaves a little differently when it comes to attaching to any surface. In order to view flagella or pili, we grew the bacteria in stationary cultures to prevent mechanical loss of flagella or pili. Once the bacteria were in in their log phase we pipetted out around 100 microliters of the culture and floated a fomvar coated nickle grid on it for about 5 mins and then let the grids dry. We found the incubation time to vary with different strains. These grids were rinsed in distilled water for 30 sec to a min. I have found that rinsing the grids was very important to remove all the debris and residual media from the grid leaving a clean prep. If you find low number of bacterial cells you can try increasing the incubation time and decreasing the rinse time. Staining: The grids were stained for 10 mins with 0.2% UA for 10 mins followed by a 1 min rinse with distilled water. The grids were then air dried before examination.
I found rinsing steps were key to a good prep. You have to play with the innoculation and rinsing steps to obtain desirable results.
Hope that helps. Good luck with your experiments.
regards, Vinod Nair Electron microscopy lab New Mexico State University Las Cruces NM 88003
ahlst007-at-umn.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Stephane, } } You do not mention the details of how you add } the bacteria to the grid. Perhaps you are } wicking too much of the bacterial sample off the } grid? } } I had this problem years ago. The investigator } claimed there were millions of billions of } bacteria in the samples, but we could not find } any on the grids. It turned out I was wicking } them all off the grid, as I would blot off most } of the sample like I would do with a virus } sample. But the bacteria are large enough to get } swept up into the stream as the sample is wicked } up with a filter paper. } } After adding a drop of sample to the grid, wick } off the excess but leave a film of sample } behind, so that looking across the grid you see } a gentle rise of sample. Then air dry, may take } 10-20 minutes, then add your stain and wick that } off as usual. One problem with this is that if } there is lots of culture media, buffer, etc, } in the sample, the cells may be buried or } contaminated when all that dries down, thought } some may be removed by the staining step. } } Another method, which results in a much cleaner } background, is to coat the Formvar coated grids } with an "adhesive" agent like poly-l-lysine or } poly-ethyleneimine. I can send details if you } want them. Then you place a grid filmed side } down onto a drop of the bacterial sample for } 5-10 minutes. Bacteria will stick to the grid. } Then transfer to drop of fixative if desired, } or to rinse buffer, then to drop of negative } stain, then blot that dry. This method usually } yields a nice even distribution of bacteria } across the grid. The flagella may be less likely } to separate from the cells as they are "glued" } down to the Formvar film along with the parent } cell body. } } Hope this helps., but let us know what works for } you. } } Gib
-- Larry Ackerman, Associate Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
==============================Original Headers============================== 33, 29 -- From Larry.Ackerman-at-ucsf.edu Tue Nov 20 16:08:48 2007 33, 29 -- Received: from emfmcb01.ucsfmedicalcenter.org (emfmcb01.ucsfmedicalcenter.org [64.54.46.97]) 33, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAKM8lvS021050 33, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Nov 2007 16:08:48 -0600 33, 29 -- Received: from 64.54.128.152 by emfmcb01.ucsfmedicalcenter.org with 33, 29 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.1.0)); 33, 29 -- Tue, 20 Nov 2007 14:25:01 -0800 33, 29 -- X-Server-Uuid: E2E48A14-EE5B-4280-A138-188440602EDD 33, 29 -- Received: from baluk.ucsf.edu ([128.218.123.88]) by exvs06.net.ucsf.edu 33, 29 -- with Microsoft SMTPSVC(6.0.3790.3959); Tue, 20 Nov 2007 14:08:39 -0800 33, 29 -- Message-ID: {47435ADE.6050004-at-ucsf.edu} 33, 29 -- Date: Tue, 20 Nov 2007 14:08:30 -0800 33, 29 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 33, 29 -- Reply-to: larry.ackerman-at-ucsf.edu 33, 29 -- Organization: UCSF, NeuroAnatomy 33, 29 -- User-Agent: Thunderbird 2.0.0.9 (Macintosh/20071031) 33, 29 -- MIME-Version: 1.0 33, 29 -- To: Microscopy-at-microscopy.com 33, 29 -- Subject: [Microscopy] Re: Visualization of bacteria flagella: 33, 29 -- troubleshooter 33, 29 -- References: {200711201616.lAKGGj2j012584-at-ns.microscopy.com} 33, 29 -- In-Reply-To: {200711201616.lAKGGj2j012584-at-ns.microscopy.com} 33, 29 -- X-OriginalArrivalTime: 20 Nov 2007 22:08:39.0143 (UTC) 33, 29 -- FILETIME=[E76D1F70:01C82BC1] 33, 29 -- X-WSS-ID: 6B5D813729O1195145-01-01 33, 29 -- Content-Type: text/plain; 33, 29 -- charset=iso-8859-1; 33, 29 -- format=flowed 33, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
one other problem that can reduce the number of bacteria seen is actually clumping of bacterial cells. So if all else fails this could maybe be another area to look at.
Some bacterial capsule or slime layers can result in large clumps of bacteria which will either look like debris, quite often stick near grid bars or else result in localised damage to the coated grid. Growth conditions of the bacteria can have a major effect on clumps, so if I have problems with particular bugs I try to get some grown on plate/slope and some in a broth. As long as the broth isn't too thick itself then this often produces the best results because the protein acts as a surfactant to give better dispersal. Ironically rinsing in water or saline rarely helps and tends to encourage clumping as well as, of course, risking damage to the pili.
I hope this is not too scientific for you folks.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: nizets2-at-yahoo.com
We have been looking with interest at the Quantomix capsules (EMS brochure) for imaging lipid droplets by SEM. Has anybody done this successfully? Does anybody have experience with these capsules they can share with me either on or off list? We'd like to hear others' experience before investing $2-3k to get it. Our private queries have yielded mixed reviews.
Thanks for your help!
Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110
==============================Original Headers============================== 6, 20 -- From tjj-at-stowers-institute.org Wed Nov 21 08:33:44 2007 6, 20 -- Received: from stowers-institute.org (mail.stowers-institute.org [204.56.6.8]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lALEXinv009875 6, 20 -- for {microscopy-at-microscopy.com} ; Wed, 21 Nov 2007 08:33:44 -0600 6, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 20 -- Content-class: urn:content-classes:message 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="US-ASCII" 6, 20 -- Subject: Quantomix capsules for SEM 6, 20 -- Date: Wed, 21 Nov 2007 08:33:23 -0600 6, 20 -- Message-ID: {C28BAF593DC3314E9C0F3A50191C2E7807A3E713-at-EXCHKC03.stowers-institute.org} 6, 20 -- X-MS-Has-Attach: 6, 20 -- X-MS-TNEF-Correlator: 6, 20 -- Thread-Topic: Quantomix capsules for SEM 6, 20 -- Thread-Index: AcgsS3hgijE6Nf9/TVSlkPgqJ0vo8w== 6, 20 -- From: "Johnson, Teri" {TJJ-at-stowers-institute.org} 6, 20 -- To: {microscopy-at-microscopy.com} 6, 20 -- Content-Transfer-Encoding: 8bit 6, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lALEXinv009875 ==============================End of - Headers==============================
Thank you very much for your reply with valuable suggestions.
About safety: Yes. My samples will be well fixed before leaving the BCL3 unit and the lab.
About immuno-labelling: I will certainly try what's suggested.
About the solution with protocol: Once my colleague has his manuscript accepted, then I will be able to post it.
Sincerely,
Fanny Chu Ultrastructural Imaging The James C Hogg iCAPTURE Lab, University of British Columbia, St. Paul's Hospital Site Rm 166, 1081 Burrard St, Vancouver, BC, Canada (604) 806-8346, x62712, x62703
} } } Leona Cohen-Gould {lcgould-at-med.cornell.edu} 11/19/07 6:12 AM } } } Dear Fanny- I don't have suggestions about the EM immuno protocol, but, having worked with a group here that studies TB I can foresee an issue: Any infected/infective materials cannot leave the level 3 bio-containment lab unless the pathogen has been totally "neutralized" (killed). For our structural studies, this required a 3 hour fixation in 2.5% glutaraldehyde. That being said, I suspect that you will have to come up with a protocol for doing at least your primary Ab labellings in the samples while they are alive and in the BCL3 unit, fixing them, and then doing the secondaries afterward, either pre-embedding, or on the embedded, sectioned material. Please post your solution to the List so we can all benefit from it. Good luck, Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Weill Cornell Medical College
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==============================Original Headers============================== 14, 19 -- From fchu-at-mrl.ubc.ca Thu Nov 22 00:52:55 2007 14, 19 -- Received: from mail.mrl.ubc.ca (mail.mrl.ubc.ca [137.82.67.155]) 14, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAM6qt9Z024648 14, 19 -- for {microscopy-at-microscopy.com} ; Thu, 22 Nov 2007 00:52:55 -0600 14, 19 -- Received: from mrlmail-MTA by mail.mrl.ubc.ca 14, 19 -- with Novell_GroupWise; Wed, 21 Nov 2007 22:52:44 -0800 14, 19 -- Message-Id: {4744B69F020000BC00023081-at-mail.mrl.ubc.ca} 14, 19 -- X-Mailer: Novell GroupWise Internet Agent 7.0.1 14, 19 -- Date: Wed, 21 Nov 2007 22:52:14 -0800 14, 19 -- From: "Fanny Chu" {fchu-at-mrl.ubc.ca} 14, 19 -- To: {lcgould-at-med.cornell.edu} , {microscopy-at-microscopy.com} 14, 19 -- Cc: {TindallR-at-missouri.edu} 14, 19 -- Subject: Re: [Microscopy] TEM co-localization of TB infected 14, 19 -- macrophages 14, 19 -- Mime-Version: 1.0 14, 19 -- Content-Type: text/plain; charset=US-ASCII 14, 19 -- Content-Disposition: inline 14, 19 -- Content-Transfer-Encoding: 8bit 14, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lAM6qt9Z024648 ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (bpoligone-at-yahoo.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, November 22, 2007 at 10:35:25 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both bpoligone-at-yahoo.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: bpoligone-at-yahoo.com Name: Brian Poligone
Organization: Yale U.
Education: Graduate College
Location: New Haven, CT
Title: Buying a Used Microscope
Question: I am looking for a trinocular(with a digital camera) microscope to read slides(as a Dermatologist). the Olympus BH-2 is comparable to what I want. I see ebay.com has some "professional" microscopes for $650(new). Are these any good? If not, how would you suggest I find a used microscope. I want to spend less than $3000.
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Email: rra-at-stowers-institute.org Name: Rhonda Allen
Organization: Stowers Institute
Title-Subject: [Filtered] Quantomix Capsules
Question: We are wondering if anyone has experience with imaging lipid droplets with the Quantomix capsules, as shown in the EMS brochure that they are sending out to anyone. I have had one comment from a user of the capsules, that these images have had a lot of photo shopping done to them to enhance contrast. Before we invest $2-3K in the system, we want to know what other people in the field are getting out of them.
I have a Hitachi 2460N that has some relays that kick in or out as I change magnification. In fact, I can hear at two different steps for a given voltage and working distance. The lower change is rather faint. There is another louder change at 10x the lower change. For example, if I hear the low change between 200 and 250x, I hear the higher change between 2000 and 2500x. I understand your 3000 will be different, but for whatever it is worth, here is what I recorded for the higher change.
The basic trend is the same as what you observed - the change comes at higher magnifications as the working distance is shortened. That makes sense if the relays are controlling the scanning coils, more magnification is had at the same deflection at shorter working distances. This makes sense with voltage too. A given scan voltage will deflect a more energetic beam less resulting in higher magnification.
Let us know what you find out.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: mcinerne-at-rose-hulman.edu [mailto:mcinerne-at-rose-hulman.edu] Sent: Thursday, November 15, 2007 8:51 PM To: wesaia-at-iastate.edu
Does anyone have a poster showing the structure of the atom they would be willing to part with? I'll pay postage.
I'm presenting a tutorial on the atom for my granddaughter's high school science class and I need a fairly simple poster to illustrate atomic structure as a lead-in to showing atomic resolution TEM images. I have a poster on crystal lattices.
Please contact me off line.
Thank you,
Ron Anderson
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I am looking for a supplier for an EL-1B 13.5 volt, 4 Amp lamp for an old Unitron inverted microscopy. All of the online suppliers I have found say they have it in stock, but it turns out to be a special order item. Can somebody please tell me who a good supplier of bulbs is?
Thanks,
Justin A. Kraft Leadership Academy West West Palm Beach, FL
==============================Original Headers============================== 3, 27 -- From kraftpiano-at-gmail.com Mon Nov 26 08:59:20 2007 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.188]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAQExJkC015294 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 26 Nov 2007 08:59:19 -0600 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so884982rvb 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 26 Nov 2007 06:59:18 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=jOEo0/EosD7vbejma3CXzvOHgiJn5r0Qgp8rgwazkPM=; 3, 27 -- b=cfUDYWf1XSn/JXo2mqTeWGsuZ+SiE2g2KnVT5jHfjmh3XuzvBVDWuablKLQZaG/rKoL/nGCYrvSIiTRfI5qRUQI4D4zLh+8ZNlGTxi9JBfYqqUp6OkiZT+wpH3DIdRVX4z2pWAfQAHU+MzZQSIIPArLgDNz/VmQvXHLY7XdCU1A= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- b=ihWrPUCXGLoDwPvgiJDume5jH7jzjNyGr7fxYUTyYnf7/lNckY5At4xwA5SMzGcr4cYpf4ow3YIkrw+jc7HCjQu/8RnjApL3l4NrA9IXA7xQ7XOsnjwcfWt3WQVfr0od6tYIuHJ5eH+xOw1ICn7m1uwKq4L3LReXV6zWtmT8I4A= 3, 27 -- Received: by 10.141.21.19 with SMTP id y19mr1181185rvi.1196089158501; 3, 27 -- Mon, 26 Nov 2007 06:59:18 -0800 (PST) 3, 27 -- Received: by 10.140.161.11 with HTTP; Mon, 26 Nov 2007 06:59:18 -0800 (PST) 3, 27 -- Message-ID: {25e2b0d20711260659w34550dd4w41722c1f0bac754f-at-mail.gmail.com} 3, 27 -- Date: Mon, 26 Nov 2007 09:59:18 -0500 3, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: Light microscopy: Lamp source. 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
I have been looking at safer ways to fill the LN2 dewers for my EDX systems. We are currently using portable dewers to fill them. Does anyone have any experience with the automatic systems? (Do you like them and are they worth the money?) Or has anyone come up with a clever way of doing this without lifting the heavy dewers? Any advice would be appreciated.
Thanks in advance, Lori Ables Solutia, Inc. Analytical Sciences Group laable-at-solutia.com
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==============================Original Headers============================== 6, 33 -- From laable-at-solutia.com Mon Nov 26 10:54:17 2007 6, 33 -- Received: from plmler9.mail.eds.com (plmler9.mail.eds.com [199.228.142.117]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAQGsHaD031249 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Nov 2007 10:54:17 -0600 6, 33 -- Received: from plmlir2.mail.eds.com (plmlir2-2.mail.eds.com [199.228.142.132]) 6, 33 -- by plmler9.mail.eds.com (8.13.8/8.13.8) with ESMTP id lAQGsGkR016375 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Nov 2007 10:54:16 -0600 6, 33 -- Received: from plmlir2.mail.eds.com (localhost [127.0.0.1]) 6, 33 -- by plmlir2.mail.eds.com (8.13.8/8.12.10) with ESMTP id lAQGs9E0001179 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Nov 2007 10:54:10 -0600 6, 33 -- Received: from mailgw2.solutia.com ([205.191.166.96]) 6, 33 -- by plmlir2.mail.eds.com (8.13.8/8.12.10) with ESMTP id lAQGs8nT001048 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Nov 2007 10:54:08 -0600 6, 33 -- Received: from USAHMSLSOIEX2.soi.dir.solutia.com ([205.191.166.99]) by mailgw2.solutia.com with Microsoft SMTPSVC(6.0.3790.3959); Mon, 26 Nov 2007 11:53:21 -0500 6, 33 -- x-mimeole: Produced By Microsoft MimeOLE V6.00.3790.4133 6, 33 -- Content-class: urn:content-classes:message 6, 33 -- MIME-Version: 1.0 6, 33 -- Content-Type: text/plain; 6, 33 -- charset="us-ascii" 6, 33 -- Subject: SEM: filling EDX dewers safely 6, 33 -- Date: Mon, 26 Nov 2007 11:53:12 -0500 6, 33 -- Message-ID: {7B97BC1F2986504EABA8A0B1C780A5C3027EE784-at-USAHMSLSOIEX2.soi.dir.solutia.com} 6, 33 -- X-MS-Has-Attach: 6, 33 -- Importance: normal 6, 33 -- X-MS-TNEF-Correlator: 6, 33 -- Priority: normal 6, 33 -- Thread-Topic: SEM: filling EDX dewers safely 6, 33 -- Thread-Index: AcgwTNSjX+lePBG/T/OXpIfrlTQwpA== 6, 33 -- From: "Ables, Lori A" {laable-at-solutia.com} 6, 33 -- To: {Microscopy-at-Microscopy.Com} 6, 33 -- X-OriginalArrivalTime: 26 Nov 2007 16:53:21.0388 (UTC) FILETIME=[DA0B4AC0:01C8304C] 6, 33 -- Content-Transfer-Encoding: 8bit 6, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lAQGsHaD031249 ==============================End of - Headers==============================
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Question: We have an older system, called Cryo-Miser, that we use here (at least 15 years old). The automatic function has not worker for years. The manual function allows use to use a hose to connect to a large 230l low pressure cylinder. It allows us to store this cylinder in a closet outside fo the SEM room and it also allows us to stay a safe distance from the EDS dewar. No worry about transporting the heavy cylinder. The best part is that there is an immediate off with a flick of a switch, to minimize splattering when the EDS dewar is full. I have no idea how much a new system costs, but the ease of use and the safety seem to be worth the while.
CoachTrack.com 32158 Camino Capistrano Suite A #429 San Juan Capistrano, CA 92675 USA
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-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Monday, November 26, 2007 7:09 AM To: Henriks-at-cox.net
I am looking for a supplier for an EL-1B 13.5 volt, 4 Amp lamp for an old Unitron inverted microscopy. All of the online suppliers I have found say they have it in stock, but it turns out to be a special order item. Can somebody please tell me who a good supplier of bulbs is?
Thanks,
Justin A. Kraft Leadership Academy West West Palm Beach, FL
==============================Original Headers============================== 3, 27 -- From kraftpiano-at-gmail.com Mon Nov 26 08:59:20 2007 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.188]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAQExJkC015294 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 26 Nov 2007 08:59:19 -0600 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so884982rvb 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 26 Nov 2007 06:59:18 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime -version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=jOEo0/EosD7vbejma3CXzvOHgiJn5r0Qgp8rgwazkPM=; 3, 27 -- b=cfUDYWf1XSn/JXo2mqTeWGsuZ+SiE2g2KnVT5jHfjmh3XuzvBVDWuablKLQZaG/rKoL/nGCYrv SIiTRfI5qRUQI4D4zLh+8ZNlGTxi9JBfYqqUp6OkiZT+wpH3DIdRVX4z2pWAfQAHU+MzZQSIIPAr LgDNz/VmQvXHLY7XdCU1A= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content -transfer-encoding:content-disposition; 3, 27 -- b=ihWrPUCXGLoDwPvgiJDume5jH7jzjNyGr7fxYUTyYnf7/lNckY5At4xwA5SMzGcr4cYpf4ow3Y Ikrw+jc7HCjQu/8RnjApL3l4NrA9IXA7xQ7XOsnjwcfWt3WQVfr0od6tYIuHJ5eH+xOw1ICn7m1u wKq4L3LReXV6zWtmT8I4A= 3, 27 -- Received: by 10.141.21.19 with SMTP id y19mr1181185rvi.1196089158501; 3, 27 -- Mon, 26 Nov 2007 06:59:18 -0800 (PST) 3, 27 -- Received: by 10.140.161.11 with HTTP; Mon, 26 Nov 2007 06:59:18 -0800 (PST) 3, 27 -- Message-ID: {25e2b0d20711260659w34550dd4w41722c1f0bac754f-at-mail.gmail.com} 3, 27 -- Date: Mon, 26 Nov 2007 09:59:18 -0500 3, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: Light microscopy: Lamp source. 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
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Dear Lori, There are a number of solutions, depending on your liquid nitrogen system. I found a low-pressure delivery system that fits into the 2.5 inch neck of my 50 liter LN2 Dewar and pressurizes the Dewar to 5 pounds. I attach a surgical tubing hose to the outlet of the delivery system and roll the Dewar to the EDX. I position the hose in the neck of the EDX Dewar and open the tap on the delivery system to fill the EDX. You can easily see when the EDX is almost full, by the LN2 spitting back out the neck. You must wait until the hose softens before you can remove it. It requires thermal gloves and eye protection, but no heavy lifting. You can find wheels and delivery systems from the same people that you buy the LN2 Dewars from. Good luck. Regards,
-----Original Message----- X-from: laable-at-solutia.com [mailto:laable-at-solutia.com] Sent: November 26, 2007 9:00 AM To: maryflet-at-interchange.ubc.ca
All,
I have been looking at safer ways to fill the LN2 dewers for my EDX systems. We are currently using portable dewers to fill them. Does anyone have any experience with the automatic systems? (Do you like them and are they worth the money?) Or has anyone come up with a clever way of doing this without lifting the heavy dewers? Any advice would be appreciated.
Thanks in advance, Lori Ables Solutia, Inc. Analytical Sciences Group laable-at-solutia.com
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This Question was submitted to Ask-A-Microscopist by (elliot.khlee-at-gmail.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, November 26, 2007 at 15:51:49 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both elliot.khlee-at-gmail.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: elliot.khlee-at-gmail.com Name: Elliot Lee
Organization: Yanbian University of Science and Technology
Education: Graduate College
Location: Yanji, Jilin Province, China
Title: setup a digital image acquisition system on Jeol 6100 SEM
Question: Hi, Recently, our materials lab got old Jeol 6100 SEM. It works alright , has no digital image acquisition system. It has only Polaroid unit. (1)We are thinking of taking video signal out and convert into digital signal and feel into computer for further process. Could you explain step by step procedure to do this?
(2)And we may want to replace Polaroid Unit with a ccd camera. Could you recommend proper ccd camera venders?
On Nov 26, 2007, at 8:54 AM, laable-at-solutia.com wrote:
} I have been looking at safer ways to fill the LN2 dewers for my EDX } systems. We are currently using portable dewers to fill them. } Does anyone have any experience with the automatic systems? (Do you } like them and are they worth the money?) Or has anyone come up } with a clever way of doing this without lifting the heavy dewers? } Any advice would be appreciated.
Dear Lori, We had a very bad experience with an automated LN2 fill system. The shutoff valve failed open on Friday evening before a long weekend-- Murphy's law, but, fortunately someone came in that weekend and discovered it--and the LN2 cooled the neck of the dewar until the vacuum seal broke, after which the concave bottom of the dewar flipped to convex. We avoided an expensive repair by constructing a tool to return the dewar to its original concave shape, but, thereafter, we gave up automating the LN2 fill and just used a portable dewar to fill the EDS dewar once a week. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Mon Nov 26 19:28:55 2007 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAR1Ssmw013637 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 26 Nov 2007 19:28:54 -0600 6, 22 -- Received: from water-dog.its.caltech.edu (water-dog [192.168.1.26]) 6, 22 -- by earth-ox-postvirus (Postfix) with ESMTP id 568661BF2A 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 26 Nov 2007 17:28:53 -0800 (PST) 6, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 6, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id 7FF3414057 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 26 Nov 2007 17:28:51 -0800 (PST) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v752.3) 6, 22 -- In-Reply-To: {200711261654.lAQGsQbI031385-at-ns.microscopy.com} 6, 22 -- References: {200711261654.lAQGsQbI031385-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 22 -- Message-Id: {19340D2C-551F-4DEF-882D-0CD10E73DA73-at-caltech.edu} 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- Subject: Re: [Microscopy] SEM: filling EDX dewers safely 6, 22 -- Date: Mon, 26 Nov 2007 17:28:55 -0800 6, 22 -- To: microscopy-at-msa.microscopy.com 6, 22 -- X-Mailer: Apple Mail (2.752.3) 6, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.4.5 ==============================End of - Headers==============================
I'm looking for opinions on monochrome CCD cameras for epifluorescence imaging, if anyone has a "pet" camera they'd like to suggest. We are running MetaMorph, v. 7.0 - I've checked the compatibility list and it didn't help me weed out any of the candidates. We don't use this system for live-cell imaging, so extreme low light sensitivity & ultra-fast capture aren't an issue. It is a shared 'scope, so the range of samples, staining intensities, & S/N from the samples is fairly broad. I'm leaning toward the Hamamatsu Orca II series & the Photometrics CoolSNAPs, but can't decide between the 2 manufacturers (I've used & been very happy with cameras from both) or between individual series members. I think I miss the old days when there were only 1 or 2 choices!
Thanks for any help/advice anyone is able to contribute. BTW, I don't know my budget limitations - I'm hoping to scavenge some "spare" money that may be left over at year's end.
We find ourselves with some extra money and I would like to get a table top carbon evaporator. Do these exist? Can anyone make any recommendations? It would be used for the basics, namely carbon coating grids, preparing carbon films, and glow discharging grids. Any help, commercial and non, would be greatly appreciated.
Thanks,
George
George P. Leser, PhD Dept. Biochemistry, Molecular Biology, and Cell Biology Northwestern University Hogan 2-100 2153 North Campus Drive Evanston, IL 60208
g-leser-at-northwestern.edu
==============================Original Headers============================== 9, 18 -- From g-leser-at-northwestern.edu Tue Nov 27 10:37:30 2007 9, 18 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57]) 9, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lARGbUOa024053 9, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 27 Nov 2007 10:37:30 -0600 9, 18 -- Received: from hal9000-3ecc03cf (dhcp-129-105-38-106.bmbcb.northwestern.edu [129.105.38.106]) 9, 18 -- by merle.it.northwestern.edu (Postfix) with ESMTP id 1C88F7570 9, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 27 Nov 2007 10:37:29 -0600 (CST) 9, 18 -- Subject: tabletop carbon evaporator 9, 18 -- Reply-To: g-leser-at-northwestern.edu 9, 18 -- Content-Type: text/plain 9, 18 -- MIME-Version: 1.0 9, 18 -- X-Mailer: Info Select 2007 (9.00.39) 9, 18 -- From: "George P. Leser" {g-leser-at-northwestern.edu} 9, 18 -- To: ListServer {Microscopy-at-microscopy.com} 9, 18 -- Date: Tue, 27 Nov 2007 10:37:31 -0600 9, 18 -- Message-Id: {20071127163729.1C88F7570-at-merle.it.northwestern.edu} 9, 18 -- Content-Transfer-Encoding: 8bit 9, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lARGbUOa024053 ==============================End of - Headers==============================
(1) You can digitze the TV out signal IF you 6100 has a true "TV" (not the Viewing CRT video out). BUt you will be very limited in resolution (640x480).
(2) You can attempt to digitize the real video signal from the scope yourself but getting the timing sync's right is problematic and you would have to hunt for the V-sync and H-sync. And you would need a A/D card and software, etc. I've attempted this with older scopes and never got good results.
(3) JEOL sells a digital imaging system for the scope (Which they call Orion) its really expensive (somewhere around $27,000 USD). JEOL used to make a system called DSG and you might find a used version somewhere but runs under Windows 95/98. Both systems are active scan systems.
(4) A European company makes another system Called Orion (was on market first actually) for digitzing older SEM's. SPI is a North American Distributor, but there others: Cost aprox. $7000 USD. It is a Passive capture system.
http://www.orionmicroscopy.com/news.htm
(5) A couple of years ago there was an article in Microscopy Today which showed using a consumer digital camera in place of the Polaroid Camera back for capturing images. This would cost the price of the camera and you do not need a high end camera as photo screen resolution will max out at just below 4MP anyway.
I have tried all the above (Except the JEOL Orion system). TV resolution is not good enough. The do it yourself never worked well. The JEOL DGS worked well but we had a noise issue with our system, and the Win 95/95 was a problem. So we got an "Original" Orion System (at 25% the JEOL system price), and have been using it for 3 years very happily.
(6) There are a number of other Vendor which offer digitizing systems for older SEM's as well.
Here's another link you should look at:
http://www.biotech.ufl.edu/EM/data/Digicheap.html
On 26 Nov 2007 at 18:12, elliot.khlee-at-gmail.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question was submitted to Ask-A-Microscopist by (elliot.khlee-at-gmail.com) } from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, November 26, 2007 at 15:51:49 } Remember to consider the Grade/Age of the student when considering the Question } --------------------------------------------------------------------------- } Please reply to both elliot.khlee-at-gmail.com as well as to the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: elliot.khlee-at-gmail.com } Name: Elliot Lee } } Organization: Yanbian University of Science and Technology } } Education: Graduate College } } Location: Yanji, Jilin Province, China } } Title: setup a digital image acquisition system on Jeol 6100 SEM } } Question: Hi, } Recently, our materials lab got old Jeol 6100 SEM. It works alright , has no digital image acquisition system. It has only Polaroid unit. (1)We are thinking of taking video signal out and convert into digital signal and feel into computer for further process. Could you explain step by step procedure to do this? } } (2)And we may want to replace Polaroid Unit with a ccd camera. Could you recommend proper ccd camera venders? } } (3) What would be the expert's suggestions? } } Thank you very much in advance. } } Elliot } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 12, 12 -- From zaluzec-at-ultra5.microscopy.com Mon Nov 26 17:11:10 2007 } 12, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 12, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAQNB9kC026105 } 12, 12 -- for {microscopy-at-microscopy.com} ; Mon, 26 Nov 2007 17:11:10 -0600 } 12, 12 -- Mime-Version: 1.0 } 12, 12 -- Message-Id: {p0624080ac37102e88c8e-at-[206.69.208.22]} } 12, 12 -- Date: Mon, 26 Nov 2007 17:11:09 -0600 } 12, 12 -- To: microscopy-at-microscopy.com } 12, 12 -- From: elliot.khlee-at-gmail.com (by way of Ask-A-Microscopist) } 12, 12 -- Subject: AskAMicroscopist: setup a digital image acquisition system on } 12, 12 -- Jeol 6100 SEM } 12, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 23, 25 -- From edelmare-at-muohio.edu Tue Nov 27 10:54:09 2007 23, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 23, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lARGs8dq004262 23, 25 -- for {microscopy-at-Microscopy.com} ; Tue, 27 Nov 2007 10:54:08 -0600 23, 25 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 23, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id lARGrxQN013571; 23, 25 -- Tue, 27 Nov 2007 11:53:59 -0500 23, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 23, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id lARGrx9w006548; 23, 25 -- Tue, 27 Nov 2007 11:53:59 -0500 23, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 23, 25 -- To: "elliot.khlee-at-gmail.com" {elliot.khlee-at-gmail.com} 23, 25 -- Date: Tue, 27 Nov 2007 11:53:59 -0500 23, 25 -- MIME-Version: 1.0 23, 25 -- Subject: Re: [Microscopy] AskAMicroscopist: setup a digital image acquisition system on 23, 25 -- CC: microscopy-at-Microscopy.com 23, 25 -- Message-ID: {474C0557.7167.5FEF174-at-edelmare.muohio.edu} 23, 25 -- Priority: normal 23, 25 -- In-reply-to: {200711262312.lAQNCI0q027259-at-ns.microscopy.com} 23, 25 -- References: {200711262312.lAQNCI0q027259-at-ns.microscopy.com} 23, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 23, 25 -- Content-type: text/plain; charset=US-ASCII 23, 25 -- Content-transfer-encoding: 7BIT 23, 25 -- Content-description: Mail message body 23, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
Energy Beam Sciences, Inc. is a US distributor for both the Polaron and Emitech line of sample preparation equipment. This includes both carbon coaters, and evaporators, for use with TEM and SEM. I will supply off-line specific information on one type of carbon Evaporator System that would work well with the applications you mentioned. And look forward to helping you in answering any further questions you may have.
Regards, Mike Dufraine EM-Product Manager Energy Beam Sciences, Inc.
g-leser-at-northwestern.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hi All, } } We find ourselves with some extra money and I would like to get a table top carbon evaporator. Do these exist? Can anyone make any recommendations? It would be used for the basics, namely carbon coating grids, preparing carbon films, and glow discharging grids. Any help, commercial and non, would be greatly appreciated. } } Thanks, } } George } } } George P. Leser, PhD } Dept. Biochemistry, Molecular Biology, and Cell Biology } Northwestern University } Hogan 2-100 } 2153 North Campus Drive } Evanston, IL 60208 } } g-leser-at-northwestern.edu } } } } ==============================Original Headers============================== } 9, 18 -- From g-leser-at-northwestern.edu Tue Nov 27 10:37:30 2007 } 9, 18 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57]) } 9, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lARGbUOa024053 } 9, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 27 Nov 2007 10:37:30 -0600 } 9, 18 -- Received: from hal9000-3ecc03cf (dhcp-129-105-38-106.bmbcb.northwestern.edu [129.105.38.106]) } 9, 18 -- by merle.it.northwestern.edu (Postfix) with ESMTP id 1C88F7570 } 9, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 27 Nov 2007 10:37:29 -0600 (CST) } 9, 18 -- Subject: tabletop carbon evaporator } 9, 18 -- Reply-To: g-leser-at-northwestern.edu } 9, 18 -- Content-Type: text/plain } 9, 18 -- MIME-Version: 1.0 } 9, 18 -- X-Mailer: Info Select 2007 (9.00.39) } 9, 18 -- From: "George P. Leser" {g-leser-at-northwestern.edu} } 9, 18 -- To: ListServer {Microscopy-at-microscopy.com} } 9, 18 -- Date: Tue, 27 Nov 2007 10:37:31 -0600 } 9, 18 -- Message-Id: {20071127163729.1C88F7570-at-merle.it.northwestern.edu} } 9, 18 -- Content-Transfer-Encoding: 8bit } 9, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lARGbUOa024053 } ==============================End of - Headers============================== }
-- Michael F. Dufraine EM - Product Manager TEL 800-992-9037 X340 MDufraine-at-ebsciences.com www.ebsciences.com
==============================Original Headers============================== 8, 26 -- From mdufraine-at-ebsciences.com Tue Nov 27 14:07:33 2007 8, 26 -- Received: from mx-outbound01.easydns.com (mailout1.easydns.com [205.210.42.66]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lARK7WWK023448 8, 26 -- for {microscopy-at-microscopy.com} ; Tue, 27 Nov 2007 14:07:33 -0600 8, 26 -- Received: from mail.ebsciences.com (unknown [69.182.224.2]) 8, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 8, 26 -- (No client certificate requested) 8, 26 -- by mx-outbound01.easydns.com (Postfix) with ESMTP id 5870548498; 8, 26 -- Tue, 27 Nov 2007 15:17:47 -0500 (EST) 8, 26 -- Received: from mdufraine.ebsciences.private ([10.10.0.190]) 8, 26 -- by mail.ebsciences.com with esmtpsa (TLSv1:AES256-SHA:256) 8, 26 -- (Exim 4.67) 8, 26 -- (envelope-from {mdufraine-at-ebsciences.com} ) 8, 26 -- id 1Ix6iR-0004Pq-Kr; Tue, 27 Nov 2007 15:07:31 -0500 8, 26 -- Message-ID: {474C7902.9080508-at-ebsciences.com} 8, 26 -- Date: Tue, 27 Nov 2007 15:07:30 -0500 8, 26 -- From: Mike Dufraine {mdufraine-at-ebsciences.com} 8, 26 -- Organization: Energy Beam Sciences 8, 26 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 8, 26 -- MIME-Version: 1.0 8, 26 -- To: g-leser-at-northwestern.edu, microscopy-at-microscopy.com 8, 26 -- Subject: Re: [Microscopy] tabletop carbon evaporator 8, 26 -- References: {200711271638.lARGcQcO025126-at-ns.microscopy.com} 8, 26 -- In-Reply-To: {200711271638.lARGcQcO025126-at-ns.microscopy.com} 8, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Mr. Edelmare, have a look at this site in Germany: http://www.pointelectronic.de/english/products/diss5_english.htm They sell a system called DISS5, which is very good and easy to adapt to an existing (older) SEM. You get quality images up to max. 4 channels / detectors parallel and up to ca. 8000x8000 pixels resolution with a Windows operating system.
...Only a satisfied user... Stefan Diller Scientific Photography, Wuerzburg, Germany
-----Original Message-----
Elliot:
(1) You can digitze the TV out signal IF you 6100 has a true "TV" (not the Viewing CRT video out). BUt you will be very limited in resolution (640x480).
(2) You can attempt to digitize the real video signal from the scope yourself but getting the timing sync's right is problematic and you would have to hunt for the V-sync and H-sync. And you would need a A/D card and software, etc. I've attempted this with older scopes and never got good results.
(3) JEOL sells a digital imaging system for the scope (Which they call Orion) its really expensive (somewhere around $27,000 USD). JEOL used to make a system called DSG and you might find a used version somewhere but runs under Windows 95/98. Both systems are active scan systems.
(4) A European company makes another system Called Orion (was on market first actually) for digitzing older SEM's. SPI is a North American Distributor, but there others: Cost aprox. $7000 USD. It is a Passive capture system.
http://www.orionmicroscopy.com/news.htm
(5) A couple of years ago there was an article in Microscopy Today which showed using a consumer digital camera in place of the Polaroid Camera back for capturing images. This would cost the price of the camera and you do not need a high end camera as photo screen resolution will max out at just below 4MP anyway.
I have tried all the above (Except the JEOL Orion system). TV resolution is not good enough. The do it yourself never worked well. The JEOL DGS worked well but we had a noise issue with our system, and the Win 95/95 was a problem. So we got an "Original" Orion System (at 25% the JEOL system price), and have been using it for 3 years very happily.
(6) There are a number of other Vendor which offer digitizing systems for older SEM's as well.
Here's another link you should look at:
http://www.biotech.ufl.edu/EM/data/Digicheap.html
On 26 Nov 2007 at 18:12, elliot.khlee-at-gmail.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question was submitted to Ask-A-Microscopist by (elliot.khlee-at-gmail.com) } from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, November 26, 2007 at 15:51:49 } Remember to consider the Grade/Age of the student when considering the Question } --------------------------------------------------------------------------- } Please reply to both elliot.khlee-at-gmail.com as well as to the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: elliot.khlee-at-gmail.com } Name: Elliot Lee } } Organization: Yanbian University of Science and Technology } } Education: Graduate College } } Location: Yanji, Jilin Province, China } } Title: setup a digital image acquisition system on Jeol 6100 SEM } } Question: Hi, } Recently, our materials lab got old Jeol 6100 SEM. It works alright , has no digital image acquisition system. It has only Polaroid unit. (1)We are thinking of taking video signal out and convert into digital signal and feel into computer for further process. Could you explain step by step procedure to do this? } } (2)And we may want to replace Polaroid Unit with a ccd camera. Could you recommend proper ccd camera venders? } } (3) What would be the expert's suggestions? } } Thank you very much in advance. } } Elliot } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 12, 12 -- From zaluzec-at-ultra5.microscopy.com Mon Nov 26 17:11:10 2007 } 12, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 12, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAQNB9kC026105 } 12, 12 -- for ; Mon, 26 Nov 2007 17:11:10 -0600 } 12, 12 -- Mime-Version: 1.0 } 12, 12 -- Message-Id: } 12, 12 -- Date: Mon, 26 Nov 2007 17:11:09 -0600 } 12, 12 -- To: microscopy-at-microscopy.com } 12, 12 -- From: elliot.khlee-at-gmail.com (by way of Ask-A-Microscopist) } 12, 12 -- Subject: AskAMicroscopist: setup a digital image acquisition system on } 12, 12 -- Jeol 6100 SEM } 12, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 23, 25 -- From edelmare-at-muohio.edu Tue Nov 27 10:54:09 2007 23, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 23, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lARGs8dq004262 23, 25 -- for ; Tue, 27 Nov 2007 10:54:08 -0600 23, 25 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 23, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id lARGrxQN013571; 23, 25 -- Tue, 27 Nov 2007 11:53:59 -0500 23, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 23, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id lARGrx9w006548; 23, 25 -- Tue, 27 Nov 2007 11:53:59 -0500 23, 25 -- From: "Richard E. Edelmann" 23, 25 -- To: "elliot.khlee-at-gmail.com" 23, 25 -- Date: Tue, 27 Nov 2007 11:53:59 -0500 23, 25 -- MIME-Version: 1.0 23, 25 -- Subject: Re: [Microscopy] AskAMicroscopist: setup a digital image acquisition system on 23, 25 -- CC: microscopy-at-Microscopy.com 23, 25 -- Message-ID: {474C0557.7167.5FEF174-at-edelmare.muohio.edu} 23, 25 -- Priority: normal 23, 25 -- In-reply-to: {200711262312.lAQNCI0q027259-at-ns.microscopy.com} 23, 25 -- References: {200711262312.lAQNCI0q027259-at-ns.microscopy.com} 23, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 23, 25 -- Content-type: text/plain; charset=US-ASCII 23, 25 -- Content-transfer-encoding: 7BIT 23, 25 -- Content-description: Mail message body 23, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
==============================Original Headers============================== 35, 27 -- From Stefan.Diller-at-t-online.de Tue Nov 27 17:07:16 2007 35, 27 -- Received: from mailout03.sul.t-online.com (mailout03.sul.t-online.de [194.25.134.81]) 35, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lARN7FwK012138 35, 27 -- for {microscopy-at-microscopy.com} ; Tue, 27 Nov 2007 17:07:16 -0600 35, 27 -- Received: from fwd35.aul.t-online.de 35, 27 -- by mailout03.sul.t-online.com with smtp 35, 27 -- id 1Ix9WH-0000Vr-03; Wed, 28 Nov 2007 00:07:09 +0100 35, 27 -- Received: from localhost (rfc8M-Zb8hUjO2-DLrAopDLnTmS8+0QZ1NBqpvtvlWz9pfmKYw5pSUL-OYZ4TptZiL-at-[172.20.101.250]) by fwd35.aul.t-online.de 35, 27 -- with esmtp id 1Ix9WE-0kv2OG0; Wed, 28 Nov 2007 00:07:06 +0100 35, 27 -- MIME-Version: 1.0 35, 27 -- In-Reply-To: {200711271658.lARGwGvL011955-at-ns.microscopy.com} 35, 27 -- References: {200711271658.lARGwGvL011955-at-ns.microscopy.com} 35, 27 -- Date: Wed, 28 Nov 2007 00:07:06 +0100 35, 27 -- Errors-To: "Stefan.Diller-at-t-online.de" {Stefan.Diller-at-t-online.de} 35, 27 -- Reply-To: "Stefan.Diller-at-t-online.de" {Stefan.Diller-at-t-online.de} 35, 27 -- To: edelmare-at-muohio.edu 35, 27 -- X-UMS: email 35, 27 -- X-Mailer: TOI Kommunikationscenter V9-1-3 35, 27 -- Subject: Re: =?ISO-8859-15?Q?=5BMicroscopy=5D?= Re: AskAMicroscopist: setup 35, 27 -- a digital image acquisition system on 35, 27 -- Cc: microscopy-at-microscopy.com 35, 27 -- From: "Stefan.Diller-at-t-online.de" {Stefan.Diller-at-t-online.de} 35, 27 -- Content-Type: text/plain; charset="ISO-8859-1" 35, 27 -- Content-Transfer-Encoding: 7bit 35, 27 -- Message-ID: {1Ix9WE-0kv2OG0-at-fwd35.aul.t-online.de} 35, 27 -- X-ID: rfc8M-Zb8hUjO2-DLrAopDLnTmS8+0QZ1NBqpvtvlWz9pfmKYw5pSUL-OYZ4TptZiL-at-t-dialin.net 35, 27 -- X-TOI-MSGID: 4805bf46-63a9-46b7-aa58-fdf28c0ae4f4 ==============================End of - Headers==============================
Connect the unit to large Dewar, insert filling probe and sensor into top of EDS Dewar via tall pole and fill is automatic. A 200L Dewar lasts weeks.
gary g.
At 08:55 AM 11/26/2007, you wrote:
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==============================Original Headers============================== 10, 18 -- From gary-at-gaugler.com Tue Nov 27 22:13:11 2007 10, 18 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lAS4DBkR030900 10, 18 -- for {microscopy-at-microscopy.com} ; Tue, 27 Nov 2007 22:13:11 -0600 10, 18 -- Message-Id: {200711280413.lAS4DBkR030900-at-ns.microscopy.com} 10, 18 -- Received: (qmail 21634 invoked from network); 27 Nov 2007 20:13:10 -0800 10, 18 -- Received: by simscan 1.1.0 ppid: 21631, pid: 21632, t: 0.1422s 10, 18 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 18 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 10, 18 -- by qsmtp4 with SMTP; 27 Nov 2007 20:13:10 -0800 10, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 18 -- Date: Tue, 27 Nov 2007 20:13:10 -0800 10, 18 -- To: laable-at-solutia.com 10, 18 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 18 -- Subject: Re: [Microscopy] SEM: filling EDX dewers safely 10, 18 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 18 -- Mime-Version: 1.0 10, 18 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-17255633 ==============================End of - Headers==============================
I was able to use the VBS automatic LN2 filling system at the previous company that I worked with. I didn't encounter any problem with it, during the time that I was there, which was around a year or so. It has been my lab improvement project so I'm glad it lasted while I'm there. ;)
The system has a sensor so it automatically fills the dewar when it reach a certain level. The dewar is located OUTSIDE the SEM laboratory which is about 7 meters away, open-air. Therefore, if the dewar needs to be filled, the gas personnel retrieve and returns the dewar from outside.
If you decide to get this, I suggest that you also get an O2 sensor which senses O2 level inside the lab and has two alarm units (inside & outside). The alarm outside the lab would guarantee that the 02 level before you get inside is safe (just in case the automatic refilling system fails and dispenses more than enough).
On the other hand, I was also able to see a certain set-up from a government lab which is inexpensive. They had a tube stuck to a dewar and exactly the right height to where the edx dewar is. Dispensing is not automatic but at least they are spared from lifting or stepping up a ladder.
Another option is to have a N2 liquid withdrawal device placed on your dewar. Either you step up the ladder by filling with a pitcher or buy a nice tube/hose. No lifting of dewar then.
Cheers,
Melina Miralles The Petroleum Institute Abu Dhabi, UAE
-----Original Message----- X-from: laable-at-solutia.com [mailto:laable-at-solutia.com] Sent: Monday, November 26, 2007 8:59 PM To: Melina Miralles
Hi all
As I have some monney to spend fast in the next weeks, I projet to buy a SL digital camera for a fixed mounting. I need a reflex body that will be put on macro bellow (I have) and be fitted with Zeiss Luminar macro lenes (I have too) for imaging samples in a magnifcation range between the macro range of a "normal" lens and the stereo microscope.
My questions are :
Are digital single lens cameras able to make a light measurement, in a MAN mode or aperture priority mode, with the lens at real aperture (without preselection) ? The lens will not be recognized as a "known" lens. How do all that automatic things deal with it ? I heared that some bodies refuse to take the photo, if they does recognise the lens ! So the MAN mode works only with lenses licensed by the manufacturer ! And I heard too that in MAN mode, only high end cameras have a light meetering which works.
Is that right ?
What are the present pet models for macro/microscpy use, in a price range from 500-1000.- euro ? If some exist in that price range !
I had a look on the Nikon D40, Canon EOS400D, and I've read something about the Pentax K100D, which is told to accept all K and 42mm mount lenses ... The Nikon D300 would be nice, and the D5 too, but much much too expensive for my use (and by the way, the monney I have !).
I tried some compact camera (Nikon coolpix 995, Canon A710IS). One can do good things, but the focusing on a little screen begin to be difficult with my eyes... And with such camera one has not much place to but a good lighting, when the camera is at 2-5 cm from the object.
Any advices ?
Thank you in advance
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
A very big thanks to all who answered my query. All were much helpful and we succeeded at the second attempt after collecting and analysing all the answers. Here is a summary of key parameters discussed in the answers and those which seem important in for success:
- Formvar films, contrary to what we thought, work really well. No need to use carbon, which is a good thing. - No need to coat with poly-L-lysin. Actually we tried with L-lysin (not poly because we don't have that product) but it seemed to us that this did not help. - We avoided the washes. Some said it was better, some said to avoid. Because we always try the lazy solutions first (personally I prefer to call them "straightforward" or "uncomplicated" ;-)), we tried without any washes and it worked. - We stained with 1% UA because we have no PTA. The staining is not strong but it is clean and it is sufficient. As one user pointed out, checking and adjusting the pH of the staining solution make sense and could help keep(ing) the flagella intact. We still have to play with that. - We tried incubating a grid with a liquid culture for 2 days. I think it may work, but at the time we did it we still had not adjusted our protocol so the results were not satisfactory. I think this may be a good choice if the preservation of the intact flagella is an issue.
Here is how we did it: we collected a colony grown on agar plate (*) and gently resuspended the bacteria in a drop of water, then added a drop of the suspension of a formvar-copper grid (held by tweezers) for 2 min. Then we briefly sucked the water with a filter paper and completed the drying under the vacuum of a sputter coater (**) A drop of 1% UA was then deposited on the grid (held by tweezers) for 1 minute then adsorbed with a filter paper and let dry on air. That's all!
Now I must say that lots of flagella are not intact, there is still room for improvement. But we just wanted to see where they were attached and how many there were per bacteria and for this purpose it was enough.
Notes (*) Starting from a liquid culture make the results much more dirty. It may work if you coat with poly-L-Lysin, then wash but we didn't try that. (**) The "drying under vacuum" was critical for the success of the protocol, god knows why (or perhaps not).
Again many thanks to the list.
Stephane
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==============================Original Headers============================== 10, 19 -- From nizets2-at-yahoo.com Wed Nov 28 09:12:57 2007 10, 19 -- Received: from web37401.mail.mud.yahoo.com (web37401.mail.mud.yahoo.com [209.191.91.133]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lASFCvq3019542 10, 19 -- for {microscopy-at-microscopy.com} ; Wed, 28 Nov 2007 09:12:57 -0600 10, 19 -- Received: (qmail 17127 invoked by uid 60001); 28 Nov 2007 15:12:56 -0000 10, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 10, 19 -- s=s1024; d=yahoo.com; 10, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 10, 19 -- b=D6Cx4qPMiRAHE8Zerzbq4FNjZvX30CI+4yPpubM0Al0YsD4nK7GJhGEGqdbIA/e1ej4XAQheUmQU1IFCyAhcaYfEKFVQcjIoSzffj0/9aw/NZtvTD+CEpfkoUJ2GLCXRe+/7Usei2aSq8SCdIU6GNvrA/s0wVAE9IAcm1fhX9To=; 10, 19 -- X-YMail-OSG: VyUT2XoVM1mo5xzqeED21qLTL3kEfRIBFnkfq25ii2eppwfiiN9lIwGbt5qn7Ha6EGzQ6y236hGACSUaYSv1OjzG_rVEkYlAS9RnxDUeLcC1DH_X5tU- 10, 19 -- Received: from [80.122.101.100] by web37401.mail.mud.yahoo.com via HTTP; Wed, 28 Nov 2007 07:12:56 PST 10, 19 -- X-Mailer: YahooMailRC/818.27 YahooMailWebService/0.7.157 10, 19 -- Date: Wed, 28 Nov 2007 07:12:56 -0800 (PST) 10, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 10, 19 -- Subject: Re: Visualling Bacterial Flagella - solved 10, 19 -- To: microscopy-at-microscopy.com 10, 19 -- MIME-Version: 1.0 10, 19 -- Content-Type: text/plain; charset=us-ascii 10, 19 -- Message-ID: {700002.16634.qm-at-web37401.mail.mud.yahoo.com} ==============================End of - Headers==============================
I just used an unopened flask of BDMA, all the rest being the same and I got my Johnnie Walker again! Thanks to those who cared to send some advice.
Regards, Stephane
On Tue, 23 Oct 2007 02:52:52 -0500 nizets2-at-yahoo.com wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all! } } I would like to wake up the list (is it hibernating?) } with the following issue. } I prepare Epon for 15 years and it was always clear, } like a good old Johnnie Walker (and almost as toxic :-D). } Today, after I added BDMA and I carefully mixed as } usual, I found it full of small dirt, like precipitates. } At the beginning I thought I mixed too strongly } (sometimes I cannot control my strength ;-)) and these } were small bubbles so I let it calm down, but to no } avail. These are definitely NOT small bubbles. } It never happened before and I wonder what I should do. } } - Do somebody have had this problem before? } - Can I use this mixture? } - What went wrong? There is no expiry date on none of } the products but they are not older than y few years } (stored at RT). I am afraid that, if I prepare it again } with the same products, I will get the same result. } } Best regards, } Stephane } } __________________________________________________ } Do You Yahoo!? } Tired of spam? Yahoo! Mail has the best spam protection } around } http://mail.yahoo.com } } } ==============================Original } Headers============================== } 6, 21 -- From nizets2-at-yahoo.com Tue Oct 23 02:51:02 2007 } 6, 21 -- Received: from web37411.mail.mud.yahoo.com } (web37411.mail.mud.yahoo.com [209.191.91.143]) } 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
Thank you very much for the info. Looks like a nice system. (Looks similar to the SIS system). Do you know an approximate price?
On 27 Nov 2007 at 17:57, Stefan.Diller-at-t-online.de wrote:
} Dear Mr. Edelmare, } have a look at this site in Germany: } http://www.pointelectronic.de/english/products/diss5_english.htm } They sell a system called DISS5, which is very good and easy to adapt to an existing (older) SEM. } You get quality images up to max. 4 channels / detectors parallel and up to ca. 8000x8000 pixels resolution with a Windows operating system. } } ...Only a satisfied user... } Stefan Diller } Scientific Photography, Wuerzburg, Germany } } } } } -----Original Message----- } Date: Tue, 27 Nov 2007 17:58:16 +0100 } Subject: [Microscopy] Re: AskAMicroscopist: setup a digital image acquisition system on } From: edelmare-at-muohio.edu } To: stefan.diller-at-t-online.de } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Elliot: } } (1) You can digitze the TV out signal IF you 6100 has a true "TV" } (not the Viewing CRT video out). BUt you will be very limited in } resolution (640x480). } } (2) You can attempt to digitize the real video signal from the scope } yourself but getting the timing sync's right is problematic and you } would have to hunt for the V-sync and H-sync. And you would need a } A/D card and software, etc. I've attempted this with older scopes } and never got good results. } } } (3) JEOL sells a digital imaging system for the scope (Which they } call Orion) its really expensive (somewhere around $27,000 USD). JEOL } used to make a system called DSG and you might find a used version } somewhere but runs under Windows 95/98. Both systems are active scan } systems. } } (http://www.jeolusa.com/PRODUCTS/ElectronOptics/ScanningElectronMicros } copesSEM/Software/OrionControlSystem/tabid/331/Default.aspx) } } } (4) A European company makes another system Called Orion (was on } market first actually) for digitzing older SEM's. SPI is a North } American Distributor, but there others: Cost aprox. $7000 USD. It } is a Passive capture system. } } http://www.orionmicroscopy.com/news.htm } } } (5) A couple of years ago there was an article in Microscopy Today } which showed using a consumer digital camera in place of the Polaroid } Camera back for capturing images. This would cost the price of the } camera and you do not need a high end camera as photo screen } resolution will max out at just below 4MP anyway. } } } I have tried all the above (Except the JEOL Orion system). TV } resolution is not good enough. The do it yourself never worked well. } The JEOL DGS worked well but we had a noise issue with our system, } and the Win 95/95 was a problem. So we got an "Original" Orion } System (at 25% the JEOL system price), and have been using it for 3 } years very happily. } } (6) There are a number of other Vendor which offer digitizing systems } for older SEM's as well. } } } Here's another link you should look at: } } http://www.biotech.ufl.edu/EM/data/Digicheap.html } } } } On 26 Nov 2007 at 18:12, elliot.khlee-at-gmail.com wrote: } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question was submitted to Ask-A-Microscopist by (elliot.khlee-at-gmail.com) } } from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, November 26, 2007 at 15:51:49 } } Remember to consider the Grade/Age of the student when considering the Question } } --------------------------------------------------------------------------- } } Please reply to both elliot.khlee-at-gmail.com as well as to the Microscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: elliot.khlee-at-gmail.com } } Name: Elliot Lee } } } } Organization: Yanbian University of Science and Technology } } } } Education: Graduate College } } } } Location: Yanji, Jilin Province, China } } } } Title: setup a digital image acquisition system on Jeol 6100 SEM } } } } Question: Hi, } } Recently, our materials lab got old Jeol 6100 SEM. It works alright , has no digital image acquisition system. It has only Polaroid unit. (1)We are thinking of taking video signal out and convert into digital signal and feel into computer for further process. Could you explain step by step procedure to do this? } } } } (2)And we may want to replace Polaroid Unit with a ccd camera. Could you recommend proper ccd camera venders? } } } } (3) What would be the expert's suggestions? } } } } Thank you very much in advance. } } } } Elliot } } } } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } } 12, 12 -- From zaluzec-at-ultra5.microscopy.com Mon Nov 26 17:11:10 2007 } } 12, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } } 12, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAQNB9kC026105 } } 12, 12 -- for {microscopy-at-microscopy.com} ; Mon, 26 Nov 2007 17:11:10 -0600 } } 12, 12 -- Mime-Version: 1.0 } } 12, 12 -- Message-Id: {p0624080ac37102e88c8e-at-[206.69.208.22]} } } 12, 12 -- Date: Mon, 26 Nov 2007 17:11:09 -0600 } } 12, 12 -- To: microscopy-at-microscopy.com } } 12, 12 -- From: elliot.khlee-at-gmail.com (by way of Ask-A-Microscopist) } } 12, 12 -- Subject: AskAMicroscopist: setup a digital image acquisition system on } } 12, 12 -- Jeol 6100 SEM } } 12, 12 -- Content-Type: text/plain; charset="us-ascii" } } ==============================End of - Headers============================== } } } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Director } 364 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } http://www.emf.muohio.edu } } } ==============================Original Headers============================== } 23, 25 -- From edelmare-at-muohio.edu Tue Nov 27 10:54:09 2007 } 23, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) } 23, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lARGs8dq004262 } 23, 25 -- for {microscopy-at-Microscopy.com} ; Tue, 27 Nov 2007 10:54:08 -0600 } 23, 25 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) } 23, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id lARGrxQN013571; } 23, 25 -- Tue, 27 Nov 2007 11:53:59 -0500 } 23, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) } 23, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id lARGrx9w006548; } 23, 25 -- Tue, 27 Nov 2007 11:53:59 -0500 } 23, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} } 23, 25 -- To: "elliot.khlee-at-gmail.com" {elliot.khlee-at-gmail.com} } 23, 25 -- Date: Tue, 27 Nov 2007 11:53:59 -0500 } 23, 25 -- MIME-Version: 1.0 } 23, 25 -- Subject: Re: [Microscopy] AskAMicroscopist: setup a digital image acquisition system on } 23, 25 -- CC: microscopy-at-Microscopy.com } 23, 25 -- Message-ID: {474C0557.7167.5FEF174-at-edelmare.muohio.edu} } 23, 25 -- Priority: normal } 23, 25 -- In-reply-to: {200711262312.lAQNCI0q027259-at-ns.microscopy.com} } 23, 25 -- References: {200711262312.lAQNCI0q027259-at-ns.microscopy.com} } 23, 25 -- X-mailer: Pegasus Mail for Windows (4.41) } 23, 25 -- Content-type: text/plain; charset=US-ASCII } 23, 25 -- Content-transfer-encoding: 7BIT } 23, 25 -- Content-description: Mail message body } 23, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 } ==============================End of - Headers============================== }
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 8, 25 -- From edelmare-at-muohio.edu Wed Nov 28 11:10:43 2007 8, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lASHAh4u014860 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 28 Nov 2007 11:10:43 -0600 8, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 8, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id lASHAbw6016315; 8, 25 -- Wed, 28 Nov 2007 12:10:37 -0500 8, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 8, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id lASHAb5S029226; 8, 25 -- Wed, 28 Nov 2007 12:10:37 -0500 8, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 8, 25 -- To: "Stefan.Diller-at-t-online.de" {Stefan.Diller-at-t-online.de} , 8, 25 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 25 -- Date: Wed, 28 Nov 2007 12:10:37 -0500 8, 25 -- MIME-Version: 1.0 8, 25 -- Subject: Re: [Microscopy] Re: AskAMicroscopist: setup a digital image acquisition system on 8, 25 -- Message-ID: {474D5ABD.3154.B3447F0-at-edelmare.muohio.edu} 8, 25 -- Priority: normal 8, 25 -- In-reply-to: {1Ix9NF-0pMILY0-at-fwd27.aul.t-online.de} 8, 25 -- References: {200711271658.lARGwGvL011955-at-ns.microscopy.com} , {1Ix9NF-0pMILY0-at-fwd27.aul.t-online.de} 8, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 8, 25 -- Content-type: text/plain; charset=US-ASCII 8, 25 -- Content-transfer-encoding: 7BIT 8, 25 -- Content-description: Mail message body 8, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
Hello Listers, I'm wondering if there are any midwesterners (I am in Ohio) out there who know someone in our region who repairs glass knifemakers. Our 30+ year old one is acting up. Thanks
==============================Original Headers============================== 3, 17 -- From mkelly-at-fs.fed.us Wed Nov 28 14:20:34 2007 3, 17 -- Received: from svmcismtp001.mci.fs.fed.us (fsmail01.fs.fed.us [165.221.108.74]) 3, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lASKKXZk032332 3, 17 -- for {microscopy-at-microscopy.com} ; Wed, 28 Nov 2007 14:20:33 -0600 3, 17 -- Received: from ([199.131.12.76]) 3, 17 -- by svmcismtp001.mci.fs.fed.us with ESMTP id KP-BRCFZ.176368052; 3, 17 -- Wed, 28 Nov 2007 14:20:04 -0600 3, 17 -- Subject: TEM-repair for a glass knife maker 3, 17 -- To: microscopy-at-microscopy.com 3, 17 -- X-Mailer: Lotus Notes Release 6.0.2CF2 July 23, 2003 3, 17 -- Message-ID: {OF928F5B2A.C0628DC8-ON852573A1.006F76DC-852573A1.006FB2A2-at-fs.fed.us} 3, 17 -- From: Mary Ellen Kelly {mkelly-at-fs.fed.us} 3, 17 -- Date: Wed, 28 Nov 2007 15:20:02 -0500 3, 17 -- X-MIMETrack: Serialize by Router on ENTWOB/E/USDAFS(Release 6.5.4FP1HF140 | September 14, 2005) at 3, 17 -- 11/28/2007 15:20:03 3, 17 -- MIME-Version: 1.0 3, 17 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Assuming you wish to stick with the Nikon F-mount SLR's to match you exisitng lenses.
Then only look at the Nikons - you can look at the user manuals for each Nikon Camera online. The SLR user manuals actually list specific limitations for each lens type - which vary for each camera body. But basiclaly you are correct: The lower priced cameras (D40, D50, D70, D80) will only work in Manual modes, The D100, D200, D300 will work in Manual or AP mode for most lenses. The D2 and D3 offer the greatest exposure control in terms of "Non-CPU" (non- autofocusing, non-autoexposure lenses).
We have used D40,D50,D70's, D100, D200, and D300 on non-cpu F-mount lenes. And yes, metering is done through the lens just like "Match- needle metering".
On 28 Nov 2007 at 7:41, jacques.faerber-at-ipcms.u-stras wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all } } As I have some monney to spend fast in the next weeks, I projet to buy a } SL digital camera for a fixed mounting. I need a reflex body that will } be put on macro bellow (I have) and be fitted with Zeiss Luminar macro } lenes (I have too) for imaging samples in a magnifcation range between } the macro range of a "normal" lens and the stereo microscope. } } My questions are : } } Are digital single lens cameras able to make a light measurement, in } a MAN mode or aperture priority mode, with the lens at real aperture } (without preselection) ? The lens will not be recognized as a "known" } lens. How do all that automatic things deal with it ? I heared that some } bodies refuse to take the photo, if they does recognise the lens ! So } the MAN mode works only with lenses licensed by the manufacturer ! And I } heard too that in MAN mode, only high end cameras have a light meetering } which works. } } Is that right ? } } What are the present pet models for macro/microscpy use, in a price } range from 500-1000.- euro ? If some exist in that price range ! } } I had a look on the Nikon D40, Canon EOS400D, and I've read something } about the Pentax K100D, which is told to accept all K and 42mm mount } lenses ... The Nikon D300 would be nice, and the D5 too, but much much } too expensive for my use (and by the way, the monney I have !). } } I tried some compact camera (Nikon coolpix 995, Canon A710IS). One can } do good things, but the focusing on a little screen begin to be } difficult with my eyes... And with such camera one has not much place to } but a good lighting, when the camera is at 2-5 cm from the object. } } Any advices ? } } } Thank you in advance } } -- } J. Faerber } IPCMS-GSI } (Institut de Physique et Chimie des Matériaux de Strasbourg } Groupe Surface et Interfaces) } 23, rue de Loess ; BP43 } 67034 Strasbourg CEDEX 2 } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)3 88 10 72 48 } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr } } } ==============================Original Headers============================== } 13, 27 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Nov 28 06:40:18 2007 } 13, 27 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.155]) } 13, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lASCeHLn003413 } 13, 27 -- for {microscopy-at-microscopy.com} ; Wed, 28 Nov 2007 06:40:17 -0600 } 13, 27 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) } 13, 27 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id lASCeDTb037059 } 13, 27 -- for {microscopy-at-microscopy.com} ; Wed, 28 Nov 2007 13:40:13 +0100 (CET) } 13, 27 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) } 13, 27 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 7BA687E4030 } 13, 27 -- for {Microscopy-at-Microscopy.Com} ; Wed, 28 Nov 2007 13:39:27 +0100 (CET) } 13, 27 -- Message-ID: {474D618E.3010201-at-ipcms.u-strasbg.fr} } 13, 27 -- Date: Wed, 28 Nov 2007 13:39:42 +0100 } 13, 27 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} } 13, 27 -- User-Agent: Thunderbird 1.5.0.14pre (X11/20071022) } 13, 27 -- MIME-Version: 1.0 } 13, 27 -- To: Microscopy-at-microscopy.com } 13, 27 -- Subject: Digital SL camera advices } 13, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 13, 27 -- Content-Transfer-Encoding: 8bit } 13, 27 -- X-IPCMS-MailScanner: Found to be clean } 13, 27 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr } 13, 27 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.155]); Wed, 28 Nov 2007 13:40:13 +0100 (CET) } 13, 27 -- X-Virus-Scanned: ClamAV 0.88.7/4936/Wed Nov 28 11:55:15 2007 on mr5.u-strasbg.fr } 13, 27 -- X-Virus-Status: Clean } 13, 27 -- X-Spam-Status: No, score=-0.0 required=5.0 tests=AWL autolearn=disabled } 13, 27 -- version=3.1.8 } 13, 27 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr5.u-strasbg.fr } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 13, 26 -- From edelmare-at-muohio.edu Wed Nov 28 15:58:27 2007 13, 26 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lASLwQ1S015540 13, 26 -- for {microscopy-at-Microscopy.com} ; Wed, 28 Nov 2007 15:58:27 -0600 13, 26 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 13, 26 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id lASLwDEY008909; 13, 26 -- Wed, 28 Nov 2007 16:58:13 -0500 13, 26 -- Received: from [192.168.1.23] ([134.53.14.105]) 13, 26 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id lASLwDm3013914; 13, 26 -- Wed, 28 Nov 2007 16:58:13 -0500 13, 26 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 13, 26 -- To: "jacques.faerber-at-ipcms.u-strasbg.fr" {jacques.faerber-at-ipcms.u-strasbg.fr} 13, 26 -- Date: Wed, 28 Nov 2007 16:58:13 -0500 13, 26 -- MIME-Version: 1.0 13, 26 -- Subject: Re: [Microscopy] Digital SL camera advices 13, 26 -- CC: microscopy-at-Microscopy.com 13, 26 -- Message-ID: {474D9E25.4000.C3B8756-at-edelmare.muohio.edu} 13, 26 -- Priority: normal 13, 26 -- In-reply-to: {200711281241.lASCfCxk004545-at-ns.microscopy.com} 13, 26 -- References: {200711281241.lASCfCxk004545-at-ns.microscopy.com} 13, 26 -- X-mailer: Pegasus Mail for Windows (4.41) 13, 26 -- Content-type: text/plain; charset=ISO-8859-1 13, 26 -- Content-description: Mail message body 13, 26 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 13, 26 -- Content-Transfer-Encoding: 8bit 13, 26 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id lASLwQ1S015540 ==============================End of - Headers==============================
Here we go again. Lori, liquid nitrogen in small transfer dewars is significantly less dangerous than crossing the street. In 35 years of handling liquid nitrogen, I have never heard of anyone being injured by the liquid nitrogen. However, I have seen equipment and floors badly damaged by malfunctioning liquid nitrogen auto fill systems, and in my own company an employee was killed by an improperly installed auto fill system. I have seen proposals from another company (mentioned in this stream) to install an auto fill system physically attached to a high resolution TEM. These people did not realize that the TEM floats and that touching it would degrade its performance. Just follow the instructions of the microscope and EDX system manufacturers. They have been doing this a long time and they know what works.
John Mardinly Intel
This is not an opinion of Intel Corporation.
-----Original Message----- X-from: laable-at-solutia.com [mailto:laable-at-solutia.com] Sent: Monday, November 26, 2007 8:54 AM To: Mardinly, John
All,
I have been looking at safer ways to fill the LN2 dewers for my EDX systems. We are currently using portable dewers to fill them. Does anyone have any experience with the automatic systems? (Do you like them and are they worth the money?) Or has anyone come up with a clever way of doing this without lifting the heavy dewers? Any advice would be appreciated.
Thanks in advance, Lori Ables Solutia, Inc. Analytical Sciences Group laable-at-solutia.com
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==============================Original Headers============================== 6, 33 -- From laable-at-solutia.com Mon Nov 26 10:54:17 2007 6, 33 -- Received: from plmler9.mail.eds.com (plmler9.mail.eds.com [199.228.142.117]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAQGsHaD031249 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Nov 2007 10:54:17 -0600 6, 33 -- Received: from plmlir2.mail.eds.com (plmlir2-2.mail.eds.com [199.228.142.132]) 6, 33 -- by plmler9.mail.eds.com (8.13.8/8.13.8) with ESMTP id lAQGsGkR016375 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Nov 2007 10:54:16 -0600 6, 33 -- Received: from plmlir2.mail.eds.com (localhost [127.0.0.1]) 6, 33 -- by plmlir2.mail.eds.com (8.13.8/8.12.10) with ESMTP id lAQGs9E0001179 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Nov 2007 10:54:10 -0600 6, 33 -- Received: from mailgw2.solutia.com ([205.191.166.96]) 6, 33 -- by plmlir2.mail.eds.com (8.13.8/8.12.10) with ESMTP id lAQGs8nT001048 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Nov 2007 10:54:08 -0600 6, 33 -- Received: from USAHMSLSOIEX2.soi.dir.solutia.com ([205.191.166.99]) by mailgw2.solutia.com with Microsoft SMTPSVC(6.0.3790.3959); Mon, 26 Nov 2007 11:53:21 -0500 6, 33 -- x-mimeole: Produced By Microsoft MimeOLE V6.00.3790.4133 6, 33 -- Content-class: urn:content-classes:message 6, 33 -- MIME-Version: 1.0 6, 33 -- Content-Type: text/plain; 6, 33 -- charset="us-ascii" 6, 33 -- Subject: SEM: filling EDX dewers safely 6, 33 -- Date: Mon, 26 Nov 2007 11:53:12 -0500 6, 33 -- Message-ID: {7B97BC1F2986504EABA8A0B1C780A5C3027EE784-at-USAHMSLSOIEX2.soi.dir.solutia. com} 6, 33 -- X-MS-Has-Attach: 6, 33 -- Importance: normal 6, 33 -- X-MS-TNEF-Correlator: 6, 33 -- Priority: normal 6, 33 -- Thread-Topic: SEM: filling EDX dewers safely 6, 33 -- Thread-Index: AcgwTNSjX+lePBG/T/OXpIfrlTQwpA== 6, 33 -- From: "Ables, Lori A" {laable-at-solutia.com} 6, 33 -- To: {Microscopy-at-Microscopy.Com} 6, 33 -- X-OriginalArrivalTime: 26 Nov 2007 16:53:21.0388 (UTC) FILETIME=[DA0B4AC0:01C8304C] 6, 33 -- Content-Transfer-Encoding: 8bit 6, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lAQGsHaD031249 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 33 -- From john.mardinly-at-intel.com Wed Nov 28 19:22:45 2007 15, 33 -- Received: from mga02.intel.com (mga02.intel.com [134.134.136.20]) 15, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAT1MiDB031614 15, 33 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 28 Nov 2007 19:22:45 -0600 15, 33 -- Received: from orsmga001.jf.intel.com ([10.7.209.18]) 15, 33 -- by orsmga101.jf.intel.com with ESMTP; 28 Nov 2007 17:22:44 -0800 15, 33 -- X-ExtLoop1: 1 15, 33 -- X-IronPort-AV: E=Sophos;i="4.23,226,1194249600"; 15, 33 -- d="scan'208";a="282182329" 15, 33 -- Received: from orsmsx335.jf.intel.com ([10.22.226.40]) 15, 33 -- by orsmga001.jf.intel.com with ESMTP; 28 Nov 2007 17:22:44 -0800 15, 33 -- Received: from orsmsx423.amr.corp.intel.com ([10.22.226.104]) by orsmsx335.jf.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 15, 33 -- Wed, 28 Nov 2007 17:22:43 -0800 15, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 15, 33 -- Content-class: urn:content-classes:message 15, 33 -- MIME-Version: 1.0 15, 33 -- Content-Type: text/plain; 15, 33 -- charset="us-ascii" 15, 33 -- Subject: RE: [Microscopy] SEM: filling EDX dewers safely 15, 33 -- Date: Wed, 28 Nov 2007 17:22:42 -0800 15, 33 -- Message-ID: {36C438BD1408B1499A19BA5730AAFC6331AC03-at-orsmsx423.amr.corp.intel.com} 15, 33 -- In-Reply-To: {200711261654.lAQGsPPM031360-at-ns.microscopy.com} 15, 33 -- X-MS-Has-Attach: 15, 33 -- X-MS-TNEF-Correlator: 15, 33 -- Thread-Topic: [Microscopy] SEM: filling EDX dewers safely 15, 33 -- Thread-Index: AcgwTQFS8QQxAwseSjimSPcb92A4tQB2A0wA 15, 33 -- References: {200711261654.lAQGsPPM031360-at-ns.microscopy.com} 15, 33 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 15, 33 -- To: {laable-at-solutia.com} 15, 33 -- Cc: {Microscopy-at-msa.microscopy.com} 15, 33 -- X-OriginalArrivalTime: 29 Nov 2007 01:22:43.0251 (UTC) FILETIME=[57290030:01C83226] 15, 33 -- Content-Transfer-Encoding: 8bit 15, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lAT1MiDB031614 ==============================End of - Headers==============================
I was told that the best way to clean SEM aperture strips is with a plasma cleaner. Can any one suggest an inexpensive one...if there is such a thing.
Or does anyone provide this as a service?
Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 6, 21 -- From dsherman-at-purdue.edu Wed Nov 28 21:01:59 2007 6, 21 -- Received: from exchange.purdue.edu (1061exfe02a.itap.purdue.edu [128.210.1.9]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAT31x3T012932 6, 21 -- for {microscopy-at-microscopy.com} ; Wed, 28 Nov 2007 21:01:59 -0600 6, 21 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by exchange.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 6, 21 -- Wed, 28 Nov 2007 21:58:23 -0500 6, 21 -- Received: from 74.140.109.252 ([74.140.109.252]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 6, 21 -- Thu, 29 Nov 2007 03:01:58 +0000 6, 21 -- User-Agent: Microsoft-Entourage/11.3.6.070618 6, 21 -- Date: Wed, 28 Nov 2007 22:01:54 -0500 6, 21 -- Subject: Cleaning aperture strips 6, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 6, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 21 -- Message-ID: {C37395D2.14F0B%dsherman-at-purdue.edu} 6, 21 -- Thread-Topic: Cleaning aperture strips 6, 21 -- Thread-Index: AcgyNDIVcKbc/54nEdy4/QAbY5Fdpg== 6, 21 -- Mime-version: 1.0 6, 21 -- Content-type: text/plain; 6, 21 -- charset="US-ASCII" 6, 21 -- Content-transfer-encoding: 7bit 6, 21 -- X-OriginalArrivalTime: 29 Nov 2007 02:58:23.0593 (UTC) FILETIME=[B4ABAD90:01C83233] ==============================End of - Headers==============================
After one year of patient work trying to convince my boss that a microwave would be a great improvement for our EM lab, we have eventually come to a point when we need propositions from suppliers. After some quick research on the internet I found only 2 suppliers namela Ted Pella and EBSciences. X-from what I have read up to now it seems that a system equipped with watercooling would fullfill our needs.
I would appreciate to receive comments of users of this product and commercial propositions off-list. The company is in Austria, please do not forget to include prices in euros! No used machines or refurbished machine, only new, shiny one ;-). It would be interesting to also know the space necessary to run such a machine, because we slowly but surely run out of space. I suppose it has to be connected to an exhaust pipe, too.
Best regards,
Stephane
____________________________________________________________________________________ Be a better sports nut! Let your teams follow you with Yahoo Mobile. Try it now. http://mobile.yahoo.com/sports;_ylt=At9_qDKvtAbMuh1G1SQtBI7ntAcJ
==============================Original Headers============================== 7, 19 -- From nizets2-at-yahoo.com Thu Nov 29 03:05:23 2007 7, 19 -- Received: from web37414.mail.mud.yahoo.com (web37414.mail.mud.yahoo.com [209.191.91.146]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lAT95MN7005887 7, 19 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 03:05:23 -0600 7, 19 -- Received: (qmail 94806 invoked by uid 60001); 29 Nov 2007 09:05:22 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 7, 19 -- b=oMUl5GcaNwiO4zoZaXDFxEJbzvTSDMqYwNM2b6bxpzxlUFSMcQjrpkzaDJtgvpiDekK3MgcSN/kR5K2d+mtsiCc/2o8BwZZO1vj9lSPQrGnjTr/6mPB/2N1p/rpYy4Y9JryHetjbTw7LmZ1xcftvMnvn62AjEDa/gV49TIxRA5M=; 7, 19 -- X-YMail-OSG: JDRm6.QVM1ktffSkNgSmm_NDFqPuZz16MklwmBmLPhcOFrAhN4ZNjvYVwEOr17b8kKT4zSS0ZPHPFM4QmAoA_i.gNF3nnm8ZfhUjT.qu2PO4rjr1dIjzKck7nDbHSQ-- 7, 19 -- Received: from [80.122.101.100] by web37414.mail.mud.yahoo.com via HTTP; Thu, 29 Nov 2007 01:05:22 PST 7, 19 -- X-Mailer: YahooMailRC/818.27 YahooMailWebService/0.7.157 7, 19 -- Date: Thu, 29 Nov 2007 01:05:22 -0800 (PST) 7, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 19 -- Subject: EM Microwave needed 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=us-ascii 7, 19 -- Message-ID: {733850.94449.qm-at-web37414.mail.mud.yahoo.com} ==============================End of - Headers==============================
Stephane, I recently learned of an Italian microwave tissue processor, Histos REM. It is made by Milestone S.r.l. www.milestonemedsrl.com
In the Western USA it is distributed by Mikron Instruments www.mikronet.com
The unit has some significant differences in design and does not require "dummy loads" such as water or a water cooler. I have not used the Milestone system and Ted Pella system I inherited is idle after several moves.
Best wishes, Larry
nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi! } } After one year of patient work trying to convince my boss that a microwave would be a great improvement for our EM lab, we have eventually come to a point when we need propositions from suppliers. } After some quick research on the internet I found only 2 suppliers namela Ted Pella and EBSciences. } X-from what I have read up to now it seems that a system equipped with watercooling would fullfill our needs. } } I would appreciate to receive comments of users of this product and commercial propositions off-list. } The company is in Austria, please do not forget to include prices in euros! } No used machines or refurbished machine, only new, shiny one ;-). } It would be interesting to also know the space necessary to run such a machine, because we slowly but surely run out of space. I suppose it has to be connected to an exhaust pipe, too. } } Best regards, } } Stephane } } } ____________________________________________________________________________________ } Be a better sports nut! Let your teams follow you } with Yahoo Mobile. Try it now. http://mobile.yahoo.com/sports;_ylt=At9_qDKvtAbMuh1G1SQtBI7ntAcJ } } ==============================Original Headers============================== } 7, 19 -- From nizets2-at-yahoo.com Thu Nov 29 03:05:23 2007 } 7, 19 -- Received: from web37414.mail.mud.yahoo.com (web37414.mail.mud.yahoo.com [209.191.91.146]) } 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lAT95MN7005887 } 7, 19 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 03:05:23 -0600 } 7, 19 -- Received: (qmail 94806 invoked by uid 60001); 29 Nov 2007 09:05:22 -0000 } 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 7, 19 -- s=s1024; d=yahoo.com; } 7, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; } 7, 19 -- b=oMUl5GcaNwiO4zoZaXDFxEJbzvTSDMqYwNM2b6bxpzxlUFSMcQjrpkzaDJtgvpiDekK3MgcSN/kR5K2d+mtsiCc/2o8BwZZO1vj9lSPQrGnjTr/6mPB/2N1p/rpYy4Y9JryHetjbTw7LmZ1xcftvMnvn62AjEDa/gV49TIxRA5M=; } 7, 19 -- X-YMail-OSG: JDRm6.QVM1ktffSkNgSmm_NDFqPuZz16MklwmBmLPhcOFrAhN4ZNjvYVwEOr17b8kKT4zSS0ZPHPFM4QmAoA_i.gNF3nnm8ZfhUjT.qu2PO4rjr1dIjzKck7nDbHSQ-- } 7, 19 -- Received: from [80.122.101.100] by web37414.mail.mud.yahoo.com via HTTP; Thu, 29 Nov 2007 01:05:22 PST } 7, 19 -- X-Mailer: YahooMailRC/818.27 YahooMailWebService/0.7.157 } 7, 19 -- Date: Thu, 29 Nov 2007 01:05:22 -0800 (PST) } 7, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 7, 19 -- Subject: EM Microwave needed } 7, 19 -- To: microscopy-at-microscopy.com } 7, 19 -- MIME-Version: 1.0 } 7, 19 -- Content-Type: text/plain; charset=us-ascii } 7, 19 -- Message-ID: {733850.94449.qm-at-web37414.mail.mud.yahoo.com} } ==============================End of - Headers============================== }
-- Larry Ackerman, Associate Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
==============================Original Headers============================== 8, 28 -- From Larry.Ackerman-at-ucsf.edu Thu Nov 29 12:15:43 2007 8, 28 -- Received: from emfmcb01.ucsfmedicalcenter.org (emfmcb01.ucsfmedicalcenter.org [64.54.46.97]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lATIFgHQ003901 8, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 12:15:43 -0600 8, 28 -- Received: from 64.54.128.152 by emfmcb01.ucsfmedicalcenter.org with 8, 28 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.1.0)); 8, 28 -- Thu, 29 Nov 2007 10:32:12 -0800 8, 28 -- X-Server-Uuid: E2E48A14-EE5B-4280-A138-188440602EDD 8, 28 -- Received: from baluk.ucsf.edu ([128.218.123.88]) by exvs06.net.ucsf.edu 8, 28 -- with Microsoft SMTPSVC(6.0.3790.3959); Thu, 29 Nov 2007 10:15:35 -0800 8, 28 -- Message-ID: {474F01C6.6040704-at-ucsf.edu} 8, 28 -- Date: Thu, 29 Nov 2007 10:15:34 -0800 8, 28 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 8, 28 -- Reply-to: larry.ackerman-at-ucsf.edu 8, 28 -- Organization: UCSF, NeuroAnatomy 8, 28 -- User-Agent: Thunderbird 2.0.0.9 (Macintosh/20071031) 8, 28 -- MIME-Version: 1.0 8, 28 -- To: nizets2-at-yahoo.com, Microscopy-at-microscopy.com 8, 28 -- Subject: Re: [Microscopy] EM Microwave needed 8, 28 -- References: {200711290913.lAT9DuRf017810-at-ns.microscopy.com} 8, 28 -- In-Reply-To: {200711290913.lAT9DuRf017810-at-ns.microscopy.com} 8, 28 -- X-OriginalArrivalTime: 29 Nov 2007 18:15:35.0223 (UTC) 8, 28 -- FILETIME=[D6140470:01C832B3] 8, 28 -- X-WSS-ID: 6B51DA2629O1774907-01-01 8, 28 -- Content-Type: text/plain; 8, 28 -- charset=iso-8859-1; 8, 28 -- format=flowed 8, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jbabiarz-at-rci.rutgers.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Pre-embebbing Immuno on tissue culture
Question: Hi Microscopists,
I have a very intersting co-culture system for myelination using DRG neurons that are non-GFG and myelinating with cells that are GFP. We have done extensive immunofluorescence experiments using the confocal to try and answer one curios problem. In some cases with certain GFP cells, myelination occurs but the GFP protein seems to be squeezed out of the wraps so that the fluorescence is minimal. A question has been raised if the cells that are doing the myelinating are truly the GFP cells or a contaminating non-GFP cell from the DRG neurons.
I am considering doing a pre-embedding immunolocalization for GFP protein using ultra small gold. Then further processing the sample and doing silver enhancement on the sections on Nickel grids. Has anyone done a technique similar to this? If so can you provide any tips or advice?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rsteabler-at-netzero.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: salem state college, recent graduate
Title-Subject: [Filtered] stereomicroscope for work with mosquitoes & cestodes
Question: I am completing two independant studies, firstly mosquito ID and cestode biology (size range of cestodes is 1 cm to 1.5 ft) i would like a reccomendation for type of stereomicroscope to purchase. I am looking at around $200 for 10x widefield not sure to get 1x&3x or 2x&4x?? halogen light with option for tran and intermit light on/off independantly finding it hard to comparison shop due to lack of info on each scope. so far i have found" celestron 44202 $251, SMP-05 $185, SMP-24 $170, SMJ-04 $230, Baytronix $160, any help would be gratefully recieved. Thank you
I want to thank all of those that replied to my question on filling the SEM with LN2. I am going to forward all the replies to the safety people and see what comes about.
Thank you, Lori Ables Solutia, Inc.
This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain Solutia confidential and privileged information. The recipient is hereby put on notice to treat the information as confidential and privileged and to not disclose or use the information except as authorized by Solutia. Any unauthorized review, printing, retention, copying, disclosure, distribution, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you received this message in error, please immediately contact the sender by reply email and delete all copies of the material from any computer. Thank you for your cooperation.
==============================Original Headers============================== 6, 33 -- From laable-at-solutia.com Thu Nov 29 12:29:18 2007 6, 33 -- Received: from ahmler1.mail.eds.com (ahmler1.mail.eds.com [192.85.154.71]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lATITHeW024249 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 Nov 2007 12:29:18 -0600 6, 33 -- Received: from ahmlir2.mail.eds.com (ahmlir2-2.mail.eds.com [192.85.154.132]) 6, 33 -- by ahmler1.mail.eds.com (8.13.8/8.13.8) with ESMTP id lATITDrl015390 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 Nov 2007 13:29:16 -0500 6, 33 -- Received: from ahmlir2.mail.eds.com (localhost [127.0.0.1]) 6, 33 -- by ahmlir2.mail.eds.com (8.13.8/8.12.10) with ESMTP id lATITABm001307 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 Nov 2007 13:29:10 -0500 6, 33 -- Received: from mailgw2.solutia.com ([205.191.166.96]) 6, 33 -- by ahmlir2.mail.eds.com (8.13.8/8.12.10) with ESMTP id lATITASI001302 6, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 Nov 2007 13:29:10 -0500 6, 33 -- Received: from USAHMSLSOIEX2.soi.dir.solutia.com ([205.191.166.99]) by mailgw2.solutia.com with Microsoft SMTPSVC(6.0.3790.3959); Thu, 29 Nov 2007 13:29:10 -0500 6, 33 -- x-mimeole: Produced By Microsoft MimeOLE V6.00.3790.4133 6, 33 -- Content-class: urn:content-classes:message 6, 33 -- MIME-Version: 1.0 6, 33 -- Content-Type: text/plain; 6, 33 -- charset="us-ascii" 6, 33 -- Subject: Thank you 6, 33 -- Date: Thu, 29 Nov 2007 13:29:06 -0500 6, 33 -- Importance: normal 6, 33 -- Priority: normal 6, 33 -- Message-ID: {7B97BC1F2986504EABA8A0B1C780A5C3027EF4D9-at-USAHMSLSOIEX2.soi.dir.solutia.com} 6, 33 -- X-MS-Has-Attach: 6, 33 -- X-MS-TNEF-Correlator: 6, 33 -- Thread-Topic: Thank you 6, 33 -- Thread-Index: AcgytbmD8lAkmmS1QXC2o1bEgHwdxA== 6, 33 -- From: "Ables, Lori A" {laable-at-solutia.com} 6, 33 -- To: {Microscopy-at-Microscopy.Com} 6, 33 -- X-OriginalArrivalTime: 29 Nov 2007 18:29:10.0279 (UTC) FILETIME=[BBE3B170:01C832B5] 6, 33 -- Content-Transfer-Encoding: 8bit 6, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lATITHeW024249 ==============================End of - Headers==============================
Hello: We have been asked to examine the structure of the microtubules in sperm tails. Our efforts to date using the standard fixations (Paraformaldehyde/glut, Osmium and UA), ethanol dehydrations and LR White embedding have not been successful in revealing fine structure. We look with increasing envy at the published shots and wonder if anyone has a protocol we could try to get through this. Thanks bob harris
Guelph Regional Integrated Imaging Facility (GRIIF) Transmission Electron Microscope Facility Dept. of Molecular and Cell Biology New Science Complex, 488 Gordon St. University of Guelph Guelph Ontario, Canada, N1G 2W1 Phone: 519-824-4120 X 56409 Fax: 519-837-1802
==============================Original Headers============================== 5, 26 -- From bharris-at-uoguelph.ca Thu Nov 29 12:50:17 2007 5, 26 -- Received: from ccshst09.cs.uoguelph.ca (ccshst09.cs.uoguelph.ca [131.104.94.206]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lATIoHuZ021359 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 12:50:17 -0600 5, 26 -- Received: from legolas.cs.uoguelph.ca (css.webmail.uoguelph.ca [131.104.93.20]) 5, 26 -- by ccshst09.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id lATIoFTj016643 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 13:50:15 -0500 5, 26 -- Received: from webmail.uoguelph.ca (localhost.localdomain [127.0.0.1]) 5, 26 -- by legolas.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id lATIoF0x013005 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 13:50:15 -0500 5, 26 -- Received: from 131.104.190.174 ([131.104.190.174]) by webmail.uoguelph.ca 5, 26 -- (Horde MIME library) with HTTP; Thu, 29 Nov 2007 13:50:15 -0500 5, 26 -- Message-ID: {20071129135015.ds9zwoe5kos04o0k-at-webmail.uoguelph.ca} 5, 26 -- Date: Thu, 29 Nov 2007 13:50:15 -0500 5, 26 -- From: Robert J Harris {bharris-at-uoguelph.ca} 5, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 26 -- Subject: TEM: Visualizing 9 + 2 microtubules in sperm tails 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; 5, 26 -- charset=ISO-8859-1; 5, 26 -- DelSp="Yes"; 5, 26 -- format="flowed" 5, 26 -- Content-Disposition: inline 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) 5, 26 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.206 ==============================End of - Headers==============================
In response to your list server question Ladd Research is also a supplier of laboratory microwaves. Please visit our web site: http://www.laddresearch.com/New_Products/Laboratory_Microwave_Ovens/laboratory_microwave_ovens.html
Best regards, John Arnott
Disclaimer: Ladd has been in the business of selling EM and lab supplies for more than 50 years.
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==============================Original Headers============================== 14, 27 -- From jd-at-laddresearch.com Thu Nov 29 12:58:51 2007 14, 27 -- Received: from arrows.electric.net (arrows.electric.net [216.129.90.200]) 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lATIwpMq001305 14, 27 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 12:58:51 -0600 14, 27 -- Received: from 1Ixob2-0007Uq-WF by arrows.electric.net with emc1-ok (Exim 4.67) 14, 27 -- (envelope-from {jd-at-laddresearch.com} ) 14, 27 -- id 1Ixob4-0007Wv-V1; Thu, 29 Nov 2007 10:58:50 -0800 14, 27 -- Received: by emcmailer; Thu, 29 Nov 2007 10:58:50 -0800 14, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 14, 27 -- by arrows.electric.net with esmtps (TLSv1:AES256-SHA:256) 14, 27 -- (Exim 4.67) 14, 27 -- (envelope-from {jd-at-laddresearch.com} ) 14, 27 -- id 1Ixob2-0007Uq-WF; Thu, 29 Nov 2007 10:58:49 -0800 14, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 14, 27 -- Date: Thu, 29 Nov 2007 13:58:20 -0500 14, 27 -- To: nizets2-at-yahoo.com 14, 27 -- From: jd {jd-at-laddresearch.com} 14, 27 -- Subject: Re: [Microscopy] EM Microwave needed 14, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 14, 27 -- In-Reply-To: {200711290912.lAT9Crvj015798-at-ns.microscopy.com} 14, 27 -- References: {200711290912.lAT9Crvj015798-at-ns.microscopy.com} 14, 27 -- Mime-Version: 1.0 14, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 14, 27 -- X-Outbound-IP: 216.204.198.170 14, 27 -- X-Env-From: jd-at-laddresearch.com 14, 27 -- X-Virus-Status: Scanned by VirusSMART (c) 14, 27 -- Message-Id: {E1Ixob4-0007Wv-V1-at-arrows.electric.net} ==============================End of - Headers==============================
Hi I have had good luck with microtubules in the flagellum of other things using PFA/Glut, Osmium, UA, EtOH and Epon. I do not like LR White for detail work. David
On Nov 29, 2007, at 11:52 AM, bharris-at-uoguelph.ca wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hello: We have been asked to examine the structure of the } microtubules in sperm tails. Our efforts to date using the standard } fixations (Paraformaldehyde/glut, Osmium and UA), ethanol dehydrations } and LR White embedding have not been successful in revealing fine } structure. We look with increasing envy at the published shots and } wonder if anyone has a protocol we could try to get through this. } Thanks bob harris } } Guelph Regional Integrated Imaging Facility (GRIIF) } Transmission Electron Microscope Facility } Dept. of Molecular and Cell Biology } New Science Complex, 488 Gordon St. } University of Guelph } Guelph Ontario, Canada, N1G 2W1 } Phone: 519-824-4120 X 56409 } Fax: 519-837-1802 } } } } } ==============================Original } Headers============================== } 5, 26 -- From bharris-at-uoguelph.ca Thu Nov 29 12:50:17 2007 } 5, 26 -- Received: from ccshst09.cs.uoguelph.ca } (ccshst09.cs.uoguelph.ca [131.104.94.206]) } 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id lATIoHuZ021359 } 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 } 12:50:17 -0600 } 5, 26 -- Received: from legolas.cs.uoguelph.ca } (css.webmail.uoguelph.ca [131.104.93.20]) } 5, 26 -- by ccshst09.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id } lATIoFTj016643 } 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 } 13:50:15 -0500 } 5, 26 -- Received: from webmail.uoguelph.ca (localhost.localdomain } [127.0.0.1]) } 5, 26 -- by legolas.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id } lATIoF0x013005 } 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 } 13:50:15 -0500 } 5, 26 -- Received: from 131.104.190.174 ([131.104.190.174]) by } webmail.uoguelph.ca } 5, 26 -- (Horde MIME library) with HTTP; Thu, 29 Nov 2007 13:50:15 } -0500 } 5, 26 -- Message-ID: } {20071129135015.ds9zwoe5kos04o0k-at-webmail.uoguelph.ca} } 5, 26 -- Date: Thu, 29 Nov 2007 13:50:15 -0500 } 5, 26 -- From: Robert J Harris {bharris-at-uoguelph.ca} } 5, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 5, 26 -- Subject: TEM: Visualizing 9 + 2 microtubules in sperm tails } 5, 26 -- MIME-Version: 1.0 } 5, 26 -- Content-Type: text/plain; } 5, 26 -- charset=ISO-8859-1; } 5, 26 -- DelSp="Yes"; } 5, 26 -- format="flowed" } 5, 26 -- Content-Disposition: inline } 5, 26 -- Content-Transfer-Encoding: 7bit } 5, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) } 5, 26 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.206 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 5, 22 -- From Elliott-at-arizona.edu Thu Nov 29 13:06:29 2007 5, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lATJ6SXm013689 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 29 Nov 2007 13:06:28 -0600 5, 22 -- Received: from gandalfs_amavis (amavis5.email.arizona.edu [10.0.0.208]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id E419F3B1240 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 29 Nov 2007 12:06:27 -0700 (MST) 5, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 6397F3B123F 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 29 Nov 2007 12:06:22 -0700 (MST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 5, 22 -- In-Reply-To: {200711291852.lATIqYMr026108-at-ns.microscopy.com} 5, 22 -- References: {200711291852.lATIqYMr026108-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {81DB6FF2-39B9-4557-BF47-E42A85A1193B-at-arizona.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: David Elliott {Elliott-at-arizona.edu} 5, 22 -- Subject: Re: [Microscopy] TEM: Visualizing 9 + 2 microtubules in sperm tails 5, 22 -- Date: Thu, 29 Nov 2007 12:06:19 -0700 5, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 22 -- X-Mailer: Apple Mail (2.752.2) 5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
We believe Ladd is the only manufacturer of apertures in the U.S. for everything from FIB's, EM's, X-Ray, to satellite thruster controls. We do not use a plasma cleaner.
If you send us more information on your strip material, etc., we will provide information on our cleaning methods. We manufacture and clean thousands of apertures per year.
Disclaimer: Ladd Researcher has been supplying apertures, lab supplies and EM products for more than 50 years
At 10:07 PM 11/28/2007, you wrote:
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==============================Original Headers============================== 15, 27 -- From jd-at-laddresearch.com Thu Nov 29 13:18:09 2007 15, 27 -- Received: from lola.electric.net (lola.electric.net [72.35.23.29]) 15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lATJI8To026385 15, 27 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 13:18:08 -0600 15, 27 -- Received: from 1Ixoti-0002ok-VO by lola.electric.net with emc1-ok (Exim 4.67) 15, 27 -- (envelope-from {jd-at-laddresearch.com} ) 15, 27 -- id 1Ixotj-0002pl-Vt; Thu, 29 Nov 2007 11:18:07 -0800 15, 27 -- Received: by emcmailer; Thu, 29 Nov 2007 11:18:07 -0800 15, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 15, 27 -- by lola.electric.net with esmtps (TLSv1:AES256-SHA:256) 15, 27 -- (Exim 4.67) 15, 27 -- (envelope-from {jd-at-laddresearch.com} ) 15, 27 -- id 1Ixoti-0002ok-VO; Thu, 29 Nov 2007 11:18:07 -0800 15, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 15, 27 -- Date: Thu, 29 Nov 2007 14:17:39 -0500 15, 27 -- To: dsherman-at-purdue.edu 15, 27 -- From: jd {jd-at-laddresearch.com} 15, 27 -- Subject: Re: [Microscopy] Cleaning aperture strips 15, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 15, 27 -- In-Reply-To: {200711290307.lAT37SXS022545-at-ns.microscopy.com} 15, 27 -- References: {200711290307.lAT37SXS022545-at-ns.microscopy.com} 15, 27 -- Mime-Version: 1.0 15, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 15, 27 -- X-Outbound-IP: 216.204.198.170 15, 27 -- X-Env-From: jd-at-laddresearch.com 15, 27 -- X-Virus-Status: Scanned by VirusSMART (c) 15, 27 -- Message-Id: {E1Ixotj-0002pl-Vt-at-lola.electric.net} ==============================End of - Headers==============================
Have you thought to localize GFP on the surface of sections cut from your tissue? We've tried two antibodies that work well: RDI (Cat# GRNFP3abg) goat anti-GFP or Abcam (ab290) rabbit anti-GFP. Either work diluted 1:50 in TRIS for 120 minutes. We used tissue embedded in LRWhite following fixation in 4% paraformaldehyde/1% glutaraldehyde in Dulbecco's media for 30 minutes on ice, rinsed twice for 15 minutes in media, then immersed in 0.1M glycine in TRIS-HCL, pH 7.4 for 60 minutes. Tissues were then dehydrated in an ice cold ethanol series to 90%, then infiltrated in 1:1; 1:2; 1:3 90% EtOH : LR White media for 45 minutes each on ice, then in two changes of ice cold LR White for 60 minutes each, then a final change of LR White at ambient temperature for 60 minutes. Polymerization was a 60C excluding oxygen.
This technique may work a little better than an enbloc procedure since you will not need to permeabilize your tissue, nor will you need to silver/gold enhance.
Best of luck,
Doug
Doug Keene Shriners Hospital Micro-Imaging Center Portland, Oregon 503-221-3434
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jbabiarz-at-rci.rutgers.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Pre-embebbing Immuno on tissue culture
Question: Hi Microscopists,
I have a very intersting co-culture system for myelination using DRG neurons that are non-GFG and myelinating with cells that are GFP. We have done extensive immunofluorescence experiments using the confocal to try and answer one curios problem. In some cases with certain GFP cells, myelination occurs but the GFP protein seems to be squeezed out of the wraps so that the fluorescence is minimal. A question has been raised if the cells that are doing the myelinating are truly the GFP cells or a contaminating non-GFP cell from the DRG neurons.
I am considering doing a pre-embedding immunolocalization for GFP protein using ultra small gold. Then further processing the sample and doing silver enhancement on the sections on Nickel grids. Has anyone done a technique similar to this? If so can you provide any tips or advice?
Thanks, Joanne
==============================Original Headers============================== 16, 27 -- From drk-at-shcc.org Thu Nov 29 13:22:29 2007 16, 27 -- Received: from mail.shcc.org (shcc.org [64.213.211.200]) 16, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lATJMThl001987 16, 27 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 Nov 2007 13:22:29 -0600 16, 27 -- Received: from por-ex-ch01.research.shcc.org (64.213.211.212) by mail.shcc.org 16, 27 -- (64.213.211.200) with Microsoft SMTP Server (TLS) id 8.0.751.0; Thu, 29 Nov 16, 27 -- 2007 11:22:25 -0800 16, 27 -- Received: from por-ex-mb01.research.shcc.org ([64.213.211.102]) by 16, 27 -- por-ex-ch01.research.shcc.org ([64.213.211.212]) with mapi; Thu, 29 Nov 2007 16, 27 -- 11:24:58 -0800 16, 27 -- From: Doug Keene {drk-at-shcc.org} 16, 27 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 16, 27 -- Date: Thu, 29 Nov 2007 11:25:01 -0800 16, 27 -- Subject: RE: Pre-embebbing Immuno on tissue culture 16, 27 -- Thread-Topic: Pre-embebbing Immuno on tissue culture 16, 27 -- Thread-Index: AcgytmRdnFpA3LMmQjaXnCfeXW1HOwABP8dg 16, 27 -- Message-ID: {145D57516E39804A832D96C2EC1C553F02EA8D12E8-at-por-ex-mb01.research.shcc.org} 16, 27 -- In-Reply-To: {200711291831.lATIVWIj031613-at-ns.microscopy.com} 16, 27 -- Accept-Language: en-US 16, 27 -- Content-Language: en-US 16, 27 -- X-MS-Has-Attach: 16, 27 -- X-MS-TNEF-Correlator: 16, 27 -- acceptlanguage: en-US 16, 27 -- Content-Type: text/plain; charset="us-ascii" 16, 27 -- MIME-Version: 1.0 16, 27 -- Content-Transfer-Encoding: 8bit 16, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lATJMThl001987 ==============================End of - Headers==============================
Every June for the past twelve years, the University of British Columbia has found (a lot of!) room for us to hold the "3D Live-Cell" course. Last year I held off on the announcement because there was a question as to whether or not the rooms that we normally use would be undergoing renovation. Even with the late start we had more students than ever before.
This year, thanks to bureaucratic delays, I am pleased to say that it now seems that we will be able to get them for one more year.
If you haven't heard of the course before, please go to
www.3dcourse.ubc.ca/2008/public.php?page=brochure (There is a PDF you can download or just read the page.)
or perhaps check out the alumni at
www.3dcourse.ubc.ca/alumni.htm to see if there is anyone you know.
There will be some new faces on the faculty this year. Here is the new list.
Holly Aaron University of California-Berkeley, CA Stephen Adams University of California-San Diego, CA Mark Cannell University of Auckland, NZ Steve Cody Ludwig Institute, Melbourne, AU Anda Cornea Oregon Health-Sciences University Ping Chin Cheng SUNY-Buffalo, NY Turan Erdogon Semrock Inc., Rochester, NY Victoria Frohlich U. Texas, HSC. San Antonio, TX Hans Gerritsen Utrecht University, NL Kurt Haas University of British Columbia, BC Stefan Hell Max Planck Institute, Goettingen, DE Iain Johnson Molecular Probes, OR Andres Kriete Tissue Informatics, Pittsburgh, PA Paul Kulesa Stowers Institute, Kansas City, MO Jennifer Lippincott-Schwartz NIH, Bethesda, MD Glen MacDonald Virginia Bloedel Hearing Inst., WA Felix Margadant University of Sydney, AU Robert Murphy Carnegie-Mellon, Pittsburgh, PA Tim Murphy University of British Columbia, BC Badri Roysam Rensselaer Polytechnic Institute, NY Michael Weis Agriculture Canada, BC
A draft listing of the program (based on 2007) can be found at
Thirteenth Annual INTERNATIONAL 12-Day Short Course on 3D Microscopy of Living Cells, June 14 - 26, 2008 (Pre-course: June 16)
Twelfth, Post-course Workshop on 3D Image Processing, June 29 -30
Organized by Prof. James Pawley, (University of Wisconsin-Madison)
in association with the Departments of Pharmacology and Physiology and the Brain Research Centre, University of British Columbia, Vancouver, BC, Canada
DATES
Applications must be received by Saturday, March 15, 2008 Deposit due Tuesday, April 15, 2008 Registration 5:00 - 7:00 PM Saturday, June 14, 2008 First Lecture 7:30 PM Saturday, June 14, 2008 Live-cell Course ends, noon Thursday, June 26, 2008 3D Image Processing Course, Saturday, June 28 - Monday, June 30, 2008
APPLICATIONS DUE BY MARCH 15, 2008
APPLICATIONS Applicants must complete a questionnaire on the web to assess knowledge level, field of interest and proposed personal, live-cell, project. But don't let this put you off: if you plan to use 3D microscopy on living cells, we can usually find a way to make it work.
Enrollment will be limited to about 36 participants (exact number depends on number of 3D Systems available). Selection will be made on the basis of background and perceived need. Those without previous LM experience will be provided with access to basic texts to read before the course begins. Application forms may be down-loaded from the WWW site at
www.3dcourse.ubc.ca/2008/public.php?page=apply, or obtained from:
Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 250 N. Mills Street, Madison, WI, 53706 JBPAWLEY-at-WISC.EDU
Additional information is available from: www.3dcourse.ubc.ca/2008/ and links.
We expect to have at least 13, 3D microscope workstations for student use and there will be an international faculty of more than 20.
Application deadlines:
Application forms should be received for screening by March 15, 2008. Successful applicants will be notified by April 1, and a deposit of 50% must be received by April 15, 2008. In general, refunds of the deposit will only be possible if someone on the waiting list can take your place but this has not been a problem in previous years. The remaining balance is due before Registration.
Pre-Course Tuition (1/2 day Basic Optical principles) $150 (US) 3D Live-cell Course Tuition (includes lunches, 2 big snacks, 3 dinners, incl. the NEW Third Edition of the Handbook of Biological Confocal Microscopy): $2,950 (US) Workshop Tuition (includes lunches, snacks, final dinner): $1,350 (US)
Room/board about $50/day (US) depending on room type.
I hope that this includes all of the information that you need, but if not, please get back to me.
Jim Pawley -- ********************************************** Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU 3D Microscopy of Living Cells Course, June 14-26, 2008, UBC, Vancouver Canada Info: www.3dcourse.ubc.ca/ Applications due by March 15, 2008 "If it ain't diffraction, it must be statistics." Anon.
==============================Original Headers============================== 35, 25 -- From jbpawley-at-wisc.edu Thu Nov 29 17:47:59 2007 35, 25 -- Received: from agogare.doit.wisc.edu (agogare.doit.wisc.edu [144.92.197.211]) 35, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lATNlxig029783 35, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 Nov 2007 17:47:59 -0600 35, 25 -- Received: from avs-daemon.smtpauth2.wiscmail.wisc.edu by 35, 25 -- smtpauth2.wiscmail.wisc.edu 35, 25 -- (Sun Java System Messaging Server 6.2-8.04 (built Feb 28 2007)) 35, 25 -- id {0JSA00L01KRY9300-at-smtpauth2.wiscmail.wisc.edu} for 35, 25 -- Microscopy-at-Microscopy.Com; Thu, 29 Nov 2007 17:47:58 -0600 (CST) 35, 25 -- Received: from [144.92.238.207] by smtpauth2.wiscmail.wisc.edu 35, 25 -- (Sun Java System Messaging Server 6.2-8.04 (built Feb 28 2007)) 35, 25 -- with ESMTPSA id {0JSA00EI4KRX1G80-at-smtpauth2.wiscmail.wisc.edu} for 35, 25 -- Microscopy-at-Microscopy.Com; Thu, 29 Nov 2007 17:47:58 -0600 (CST) 35, 25 -- Date: Thu, 29 Nov 2007 17:47:53 -0600 35, 25 -- From: James Pawley {jbpawley-at-wisc.edu} 35, 25 -- Subject: LM: Thirteenth International UBC 3D LiveCell Course, June 14-26, 2008 35, 25 -- To: Microscopy-at-Microscopy.Com 35, 25 -- Message-id: {p06240802c374ff09f0b3-at-[144.92.238.207]} 35, 25 -- MIME-version: 1.0 35, 25 -- Content-type: text/plain; charset=us-ascii; format=flowed 35, 25 -- Content-transfer-encoding: 7BIT 35, 25 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=144.92.238.207 35, 25 -- X-Spam-PmxInfo: Server=avs-10, Version=5.3.3.310218, 35, 25 -- Antispam-Engine: 2.5.2.313940, Antispam-Data: 2007.11.29.152926, 35, 25 -- SenderIP=144.92.238.207 ==============================End of - Headers==============================
I would also recommend Epon embedding (do not mix EthOH with Epon, but change in acetone or acetonitrile before Epon impregnation). You could also try tannic acid. One friend of mine who studies microtubules has recently told me that he fixes in microwave before doing all the rest classically and he saw a clear improvement. However you need some time to find the right conditions in the microwave.
Stephane
----- Original Message ---- X-from: "bharris-at-uoguelph.ca" {bharris-at-uoguelph.ca} To: nizets2-at-yahoo.com Sent: Thursday, November 29, 2007 7:53:40 PM
Hello: We have been asked to examine the structure of the microtubules in sperm tails. Our efforts to date using the standard fixations (Paraformaldehyde/glut, Osmium and UA), ethanol dehydrations and LR White embedding have not been successful in revealing fine structure. We look with increasing envy at the published shots and wonder if anyone has a protocol we could try to get through this. Thanks bob harris
Guelph Regional Integrated Imaging Facility (GRIIF) Transmission Electron Microscope Facility Dept. of Molecular and Cell Biology New Science Complex, 488 Gordon St. University of Guelph Guelph Ontario, Canada, N1G 2W1 Phone: 519-824-4120 X 56409 Fax: 519-837-1802
==============================Original Headers============================== 5, 26 -- From bharris-at-uoguelph.ca Thu Nov 29 12:50:17 2007 5, 26 -- Received: from ccshst09.cs.uoguelph.ca (ccshst09.cs.uoguelph.ca [131.104.94.206]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lATIoHuZ021359 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 12:50:17 -0600 5, 26 -- Received: from legolas.cs.uoguelph.ca (css.webmail.uoguelph.ca [131.104.93.20]) 5, 26 -- by ccshst09.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id lATIoFTj016643 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 13:50:15 -0500 5, 26 -- Received: from webmail.uoguelph.ca (localhost.localdomain [127.0.0.1]) 5, 26 -- by legolas.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id lATIoF0x013005 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 13:50:15 -0500 5, 26 -- Received: from 131.104.190.174 ([131.104.190.174]) by webmail.uoguelph.ca 5, 26 -- (Horde MIME library) with HTTP; Thu, 29 Nov 2007 13:50:15 -0500 5, 26 -- Message-ID: {20071129135015.ds9zwoe5kos04o0k-at-webmail.uoguelph.ca} 5, 26 -- Date: Thu, 29 Nov 2007 13:50:15 -0500 5, 26 -- From: Robert J Harris {bharris-at-uoguelph.ca} 5, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 26 -- Subject: TEM: Visualizing 9 + 2 microtubules in sperm tails 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; 5, 26 -- charset=ISO-8859-1; 5, 26 -- DelSp="Yes"; 5, 26 -- format="flowed" 5, 26 -- Content-Disposition: inline 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) 5, 26 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.206 ==============================End of - Headers==============================
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==============================Original Headers============================== 16, 20 -- From nizets2-at-yahoo.com Fri Nov 30 01:58:52 2007 16, 20 -- Received: from web37401.mail.mud.yahoo.com (web37401.mail.mud.yahoo.com [209.191.91.133]) 16, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lAU7wqce019742 16, 20 -- for {microscopy-at-microscopy.com} ; Fri, 30 Nov 2007 01:58:52 -0600 16, 20 -- Received: (qmail 41103 invoked by uid 60001); 30 Nov 2007 07:58:51 -0000 16, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 16, 20 -- s=s1024; d=yahoo.com; 16, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 16, 20 -- b=N8X0uNEekwjkWWQUraTqKKHnNJaBWd+oVd7Lzh4jGKgumYMOMn6C0lVAlcH8yU1DJ/kJZ7tbRjTGCsVgh/CyrHIxAHsE02M5oQGwPRm1P1VQ8bIOFW6WT3IC7q1vcybPkstr9O8mrLhubE6BNLoiyEXcplIUHKbhgUKK/cCnyEo=; 16, 20 -- X-YMail-OSG: SBGEDa0VM1k2BQTVQ8Ky66FtjqjMiAbQfnG1_TAEU4zVOCygQ4h9RST21A9XO8PsnVTxD_E.7K4Q8r761l6dyt7Oy44mYJZAUz7aK855W8ZVFAbOV2hUYhqPC1vuT88tW926le9i4400MOi14Z5omePVg2ExuFmVv8oA7hrRP0TN 16, 20 -- Received: from [80.122.101.100] by web37401.mail.mud.yahoo.com via HTTP; Thu, 29 Nov 2007 23:58:51 PST 16, 20 -- X-Mailer: YahooMailRC/818.27 YahooMailWebService/0.7.157 16, 20 -- Date: Thu, 29 Nov 2007 23:58:51 -0800 (PST) 16, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 16, 20 -- Subject: Re: [Microscopy] TEM: Visualizing 9 + 2 microtubules in sperm tails 16, 20 -- To: bharris-at-uoguelph.ca 16, 20 -- Cc: microscopy-at-microscopy.com 16, 20 -- MIME-Version: 1.0 16, 20 -- Content-Type: text/plain; charset=us-ascii 16, 20 -- Message-ID: {809061.31996.qm-at-web37401.mail.mud.yahoo.com} ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ehaller-at-health.usf.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ehaller-at-health.usf.edu Name: Edward Haller
Organization: University of South Florida
Title-Subject: [Filtered] Philips 515 SEM available for parts
Question: The University of South Florida Medical school is in the process of updating our Microscopy Core. We have a non-functioning Philips 515 SEM (vintage 1985)available for parts. Anyone interested can have the microscope if they pay for disassembly/moving the instrument. The column is still under vacuum. A malfunction in the scan/image formation system rendered the microscope unuseable. FEI said it would cost ~$10k to repair the problem, and replacement parts are hard to come by. The SEM has a quad scintillator backscattered detector that work quite well, and signal mixing capabiilty. It was under contract until fall of 2005. The monitors on the scope are good.
Edward Haller, Lab Manager Microscopy and Cell Imaging Core USF Health Tampa, FL 33612 813-974-0569 (W) 813-949-9896 (H)
I no longer have the references in front of me, but there is a long history of using tannic acid to enhance the preservation and visualization of microtubules. Do a Google search, or better yet, search PubMed. The references are there. Since it has been years since I did this, I don't remember percentages or times. I do remember buying a Mallinkrodt tannic acid as this is the only one that was recommended. Check Hayat or one of the other books as a reference.
Roger Moretz, Ph.D. Retired but not dead yet
On Nov 29, 2007 1:53 PM, {bharris-at-uoguelph.ca} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello: We have been asked to examine the structure of the } microtubules in sperm tails. Our efforts to date using the standard } fixations (Paraformaldehyde/glut, Osmium and UA), ethanol dehydrations } and LR White embedding have not been successful in revealing fine } structure. We look with increasing envy at the published shots and } wonder if anyone has a protocol we could try to get through this. } Thanks bob harris } } Guelph Regional Integrated Imaging Facility (GRIIF) } Transmission Electron Microscope Facility } Dept. of Molecular and Cell Biology } New Science Complex, 488 Gordon St. } University of Guelph } Guelph Ontario, Canada, N1G 2W1 } Phone: 519-824-4120 X 56409 } Fax: 519-837-1802 } } } } } ==============================Original Headers============================== } 5, 26 -- From bharris-at-uoguelph.ca Thu Nov 29 12:50:17 2007 } 5, 26 -- Received: from ccshst09.cs.uoguelph.ca (ccshst09.cs.uoguelph.ca [131.104.94.206]) } 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lATIoHuZ021359 } 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 12:50:17 -0600 } 5, 26 -- Received: from legolas.cs.uoguelph.ca (css.webmail.uoguelph.ca [131.104.93.20]) } 5, 26 -- by ccshst09.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id lATIoFTj016643 } 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 13:50:15 -0500 } 5, 26 -- Received: from webmail.uoguelph.ca (localhost.localdomain [127.0.0.1]) } 5, 26 -- by legolas.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id lATIoF0x013005 } 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 13:50:15 -0500 } 5, 26 -- Received: from 131.104.190.174 ([131.104.190.174]) by webmail.uoguelph.ca } 5, 26 -- (Horde MIME library) with HTTP; Thu, 29 Nov 2007 13:50:15 -0500 } 5, 26 -- Message-ID: {20071129135015.ds9zwoe5kos04o0k-at-webmail.uoguelph.ca} } 5, 26 -- Date: Thu, 29 Nov 2007 13:50:15 -0500 } 5, 26 -- From: Robert J Harris {bharris-at-uoguelph.ca} } 5, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 5, 26 -- Subject: TEM: Visualizing 9 + 2 microtubules in sperm tails } 5, 26 -- MIME-Version: 1.0 } 5, 26 -- Content-Type: text/plain; } 5, 26 -- charset=ISO-8859-1; } 5, 26 -- DelSp="Yes"; } 5, 26 -- format="flowed" } 5, 26 -- Content-Disposition: inline } 5, 26 -- Content-Transfer-Encoding: 7bit } 5, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) } 5, 26 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.206 } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 29 -- From rcmoretz-at-gmail.com Fri Nov 30 16:47:17 2007 4, 29 -- Received: from nf-out-0910.google.com (nf-out-0910.google.com [64.233.182.190]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lAUMlGPE024896 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Nov 2007 16:47:16 -0600 4, 29 -- Received: by nf-out-0910.google.com with SMTP id d3so2977661nfc 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Nov 2007 14:47:16 -0800 (PST) 4, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 29 -- d=gmail.com; s=gamma; 4, 29 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 4, 29 -- bh=70H7NlYFyEBGWLhmKkrPMQO4NoR3qGn9anSxqVeACRk=; 4, 29 -- b=jlAQsboYw3oJukZg/Ws0bmT++M4VJLQQEosM53waf2o7luVQPL/rocwz//XeKh6i3FsMbIWpxQYT9UJZWZnCsxAUIPQDsCFzWsd5PHnCsWyYt5xxzhsy5JX4imKQZOSgJ7FdPPvYr3+9R5sgFdOf5z+j0ir+LCF3odaD0/CHhH8= 4, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 29 -- d=gmail.com; s=gamma; 4, 29 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 4, 29 -- b=KrnYpeez1rSsxIE9HM2YTxRtY0/YWdM283HyUXfsxNDppMr090JD+NxEsY9A+5ME6fvbPXe7N/5VMF2e9UIYYWEjOxLEAwB3TOZ+1NfGvLL79kcEu/any/iIYOk7Ij1QwiLcvcbPnfyYvgUeayX2VbV/C/gmPFeQR5PHjtq2CAk= 4, 29 -- Received: by 10.78.122.16 with SMTP id u16mr9525335huc.1196462835574; 4, 29 -- Fri, 30 Nov 2007 14:47:15 -0800 (PST) 4, 29 -- Received: by 10.78.97.6 with HTTP; Fri, 30 Nov 2007 14:47:15 -0800 (PST) 4, 29 -- Message-ID: {950e3cfd0711301447l33234ec4off37859c77ea6d75-at-mail.gmail.com} 4, 29 -- Date: Fri, 30 Nov 2007 17:47:15 -0500 4, 29 -- From: "Roger Moretz" {rcmoretz-at-gmail.com} 4, 29 -- To: bharris-at-uoguelph.ca, "Microscopy Listserv" {Microscopy-at-microscopy.com} 4, 29 -- Subject: Re: [Microscopy] TEM: Visualizing 9 + 2 microtubules in sperm tails 4, 29 -- In-Reply-To: {200711291853.lATIrjqs028813-at-ns.microscopy.com} 4, 29 -- MIME-Version: 1.0 4, 29 -- Content-Type: text/plain; charset=ISO-8859-1 4, 29 -- Content-Transfer-Encoding: 7bit 4, 29 -- Content-Disposition: inline 4, 29 -- References: {200711291853.lATIrjqs028813-at-ns.microscopy.com} ==============================End of - Headers==============================
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