A quick Google search came up with over 42K references. The relevant ones are Tilney et al, J Cell Biol, vol 59, 1973 (or was that 1975?) and Mizuhira and Futaeska (I don't have the complete reference). I think the Tilney paper has the proportions, methods, etc. I didn't do a PubMed search since the Google search gave me the basic info I needed. Hope this helps.
Roger Moretz, Ph.D. Retired but not dead yet
On Nov 30, 2007 5:47 PM, Roger Moretz {rcmoretz-at-gmail.com} wrote: } Brian: } } I no longer have the references in front of me, but there is a long } history of using tannic acid to enhance the preservation and } visualization of microtubules. Do a Google search, or better yet, } search PubMed. The references are there. Since it has been years } since I did this, I don't remember percentages or times. I do } remember buying a Mallinkrodt tannic acid as this is the only one that } was recommended. Check Hayat or one of the other books as a } reference. } } Roger Moretz, Ph.D. } Retired but not dead yet } } } On Nov 29, 2007 1:53 PM, {bharris-at-uoguelph.ca} wrote: } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hello: We have been asked to examine the structure of the } } microtubules in sperm tails. Our efforts to date using the standard } } fixations (Paraformaldehyde/glut, Osmium and UA), ethanol dehydrations } } and LR White embedding have not been successful in revealing fine } } structure. We look with increasing envy at the published shots and } } wonder if anyone has a protocol we could try to get through this. } } Thanks bob harris } } } } Guelph Regional Integrated Imaging Facility (GRIIF) } } Transmission Electron Microscope Facility } } Dept. of Molecular and Cell Biology } } New Science Complex, 488 Gordon St. } } University of Guelph } } Guelph Ontario, Canada, N1G 2W1 } } Phone: 519-824-4120 X 56409 } } Fax: 519-837-1802 } } } } } } } } } } ==============================Original Headers============================== } } 5, 26 -- From bharris-at-uoguelph.ca Thu Nov 29 12:50:17 2007 } } 5, 26 -- Received: from ccshst09.cs.uoguelph.ca (ccshst09.cs.uoguelph.ca [131.104.94.206]) } } 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lATIoHuZ021359 } } 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 12:50:17 -0600 } } 5, 26 -- Received: from legolas.cs.uoguelph.ca (css.webmail.uoguelph.ca [131.104.93.20]) } } 5, 26 -- by ccshst09.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id lATIoFTj016643 } } 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 13:50:15 -0500 } } 5, 26 -- Received: from webmail.uoguelph.ca (localhost.localdomain [127.0.0.1]) } } 5, 26 -- by legolas.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id lATIoF0x013005 } } 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 29 Nov 2007 13:50:15 -0500 } } 5, 26 -- Received: from 131.104.190.174 ([131.104.190.174]) by webmail.uoguelph.ca } } 5, 26 -- (Horde MIME library) with HTTP; Thu, 29 Nov 2007 13:50:15 -0500 } } 5, 26 -- Message-ID: {20071129135015.ds9zwoe5kos04o0k-at-webmail.uoguelph.ca} } } 5, 26 -- Date: Thu, 29 Nov 2007 13:50:15 -0500 } } 5, 26 -- From: Robert J Harris {bharris-at-uoguelph.ca} } } 5, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } } 5, 26 -- Subject: TEM: Visualizing 9 + 2 microtubules in sperm tails } } 5, 26 -- MIME-Version: 1.0 } } 5, 26 -- Content-Type: text/plain; } } 5, 26 -- charset=ISO-8859-1; } } 5, 26 -- DelSp="Yes"; } } 5, 26 -- format="flowed" } } 5, 26 -- Content-Disposition: inline } } 5, 26 -- Content-Transfer-Encoding: 7bit } } 5, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) } } 5, 26 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.206 } } ==============================End of - Headers============================== } } }
==============================Original Headers============================== 4, 30 -- From rcmoretz-at-gmail.com Sun Dec 2 18:37:42 2007 4, 30 -- Received: from mu-out-0910.google.com (mu-out-0910.google.com [209.85.134.189]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB30bfMd024993 4, 30 -- for {Microscopy-at-microscopy.com} ; Sun, 2 Dec 2007 18:37:42 -0600 4, 30 -- Received: by mu-out-0910.google.com with SMTP id i2so363780mue 4, 30 -- for {Microscopy-at-microscopy.com} ; Sun, 02 Dec 2007 16:37:41 -0800 (PST) 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 4, 30 -- bh=dWhNlJzEHCQmdBJIIwQ2nBMdO0b5JnBO1tWisUh3Z9s=; 4, 30 -- b=lrAGAawzjsvKRZnu5DrCThMxrlPC/+0sdZQKMXZ02aP/BjRid74Roxvy/QZNAjbJUdRdFrosNBEwZLxQbVsAUgO6cnAzREIPii9zJc3r0AskLCTHm4L9vakMYw1rOVT4RdplejF1EaJpJ9bmsJNH08+MuMAaF43nLUZo9yBCc1I= 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 4, 30 -- b=cRETnZQvSSdQwMSQrAF3ivrCWoDlF2tlmmz5XXQWQuMTgtKBbliXL/46bd8648jQxBIYks5dcSa+37psXeTr7TUVbZ2QkYRJy642h33VT3AV/Ks9cIPfoza6DTLU+MwEucpFuurvfbC/0BxMHSAmx8oYDxoLPVzMJ6ZcW6m6C/c= 4, 30 -- Received: by 10.78.165.16 with SMTP id n16mr2148057hue.1196642260193; 4, 30 -- Sun, 02 Dec 2007 16:37:40 -0800 (PST) 4, 30 -- Received: by 10.78.100.4 with HTTP; Sun, 2 Dec 2007 16:37:40 -0800 (PST) 4, 30 -- Message-ID: {950e3cfd0712021637v4ad64540pb80413e3b72b0c95-at-mail.gmail.com} 4, 30 -- Date: Sun, 2 Dec 2007 19:37:40 -0500 4, 30 -- From: "Roger Moretz" {rcmoretz-at-gmail.com} 4, 30 -- To: bharris-at-uoguelph.ca, "Microscopy Listserv" {Microscopy-at-microscopy.com} 4, 30 -- Subject: Re: [Microscopy] TEM: Visualizing 9 + 2 microtubules in sperm tails 4, 30 -- In-Reply-To: {950e3cfd0711301447l33234ec4off37859c77ea6d75-at-mail.gmail.com} 4, 30 -- MIME-Version: 1.0 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 4, 30 -- Content-Transfer-Encoding: 7bit 4, 30 -- Content-Disposition: inline 4, 30 -- References: {200711291853.lATIrjqs028813-at-ns.microscopy.com} 4, 30 -- {950e3cfd0711301447l33234ec4off37859c77ea6d75-at-mail.gmail.com} ==============================End of - Headers==============================
MSA's Project MICRO is a middle school outreach program. Its website has a lot of information, including an extensive bibliography of books, videos, DVDs & CDs, and websites. That list has just been updated, and it's worth a visit, even if you don't do organized "outreach". Go to http://www.microscopy.org/ProjectMICRO and try "2007" as your search category, for example. You'll find that this year saw the publication of the 4th revised printing of MICRO's "Microscopic Explorations", an inexpensive 432 page atlas of colorized SEM imasges, an outstanding history of microscopes, and a FREE 36 page pamphlet about optics as a career (sponsored by the Optical Society of America and the Girl Scouts of America), available online as a pdf.
A new search category has been added this year: books in Spanish. Forget about politics, and consider that teachers and volunteers who want to teach science classes and workshops have a tough time when the students include a lot of English learners. If you search the list for "Spanish language", you'll find two excellent pairs of books (one English, one Spanish, same text), and a bilingual Optical Society of America "optics for kids" website.
No search is perfect; MICRO always welcomes information on in-print publications that have been missed.
-- Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 4, 16 -- From schooley-at-mcn.org Mon Dec 3 12:25:15 2007 4, 16 -- Received: from dns4.mcn.org (dns4.mcn.org [216.150.240.31]) 4, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB3IPEnX001543 4, 16 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 3 Dec 2007 12:25:14 -0600 4, 16 -- Received: from [66.81.75.159] (helo=[10.0.1.2]) 4, 16 -- by dns4.mcn.org with esmtpa (Exim 4.60) 4, 16 -- (envelope-from {schooley-at-mcn.org} ) 4, 16 -- id JSHKHZ-000JWN-4Y 4, 16 -- for Microscopy-at-MSA.Microscopy.Com; Mon, 03 Dec 2007 10:25:13 -0800 4, 16 -- Mime-Version: 1.0 4, 16 -- Message-Id: {a06200706c379fa8652eb-at-[10.0.1.2]} 4, 16 -- Date: Mon, 3 Dec 2007 10:29:49 -0800 4, 16 -- To: Microscopy-at-MSA.Microscopy.Com 4, 16 -- From: Caroline Schooley {schooley-at-mcn.org} 4, 16 -- Subject: [Microscopy] education 4, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I tried to prepare wood (Fagus and Pinus)for transmission electron microscopy by embedding in Epon and LR-White and got terrible problems in sectioning. The blocks seemed ok, they were not soft. We got sections, but they immersed in the trough and did not stretch. The result was aweful. I do not understand what went wrong, maybe, poor infiltration? Is there anybody who has experience with wooden material?
Best regards, Anne
==============================Original Headers============================== 4, 30 -- From heller-at-uni-hohenheim.de Mon Dec 3 13:13:15 2007 4, 30 -- Received: from smtp1.rz.uni-hohenheim.de (smtp1.rz.uni-hohenheim.de [144.41.4.41]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB3JDF8N015553 4, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 13:13:15 -0600 4, 30 -- Received: from localhost (smtp2.rz.uni-hohenheim.de [144.41.4.42]) 4, 30 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id B0C7F11D3A7 4, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 20:13:13 +0100 (CET) 4, 30 -- X-Virus-Scanned: amavisd-new at uni-hohenheim.de 4, 30 -- Received: from smtp1.rz.uni-hohenheim.de ([144.41.4.41]) 4, 30 -- by localhost (webmail3.rz.uni-hohenheim.de [144.41.4.42]) (amavisd-new, port 10024) 4, 30 -- with ESMTP id 7y28ue8YeTG0 for {Microscopy-at-microscopy.com} ; 4, 30 -- Mon, 3 Dec 2007 20:13:06 +0100 (CET) 4, 30 -- Received: from webmail.uni-hohenheim.de (webmail2.rz.uni-hohenheim.de [144.41.4.31]) 4, 30 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 6EDB611D3D8 4, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 20:13:05 +0100 (CET) 4, 30 -- Received: by webmail.uni-hohenheim.de (Postfix, from userid 48) 4, 30 -- id B726C60153; Mon, 3 Dec 2007 20:13:04 +0100 (CET) 4, 30 -- Received: from jatropha.botanik.uni-hohenheim.de (jatropha.botanik.uni-hohenheim.de [144.41.33.113]) 4, 30 -- by webmail.uni-hohenheim.de (IMP) with HTTP 4, 30 -- for {heller-at-10.0.0.5} ; Mon, 3 Dec 2007 20:13:04 +0100 4, 30 -- Message-ID: {1196709184.475455409f6cb-at-webmail.uni-hohenheim.de} 4, 30 -- Date: Mon, 3 Dec 2007 20:13:04 +0100 4, 30 -- From: heller-at-uni-hohenheim.de 4, 30 -- To: Microscopy-at-microscopy.com 4, 30 -- Subject: preparation of wood for TEM 4, 30 -- MIME-Version: 1.0 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 4, 30 -- Content-Transfer-Encoding: 8bit 4, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2.2 4, 30 -- X-Originating-IP: 144.41.33.113 ==============================End of - Headers==============================
I am resurrecting a Balzers 301 freeze fracture unit. The coating and vacuum units seem to be working ok. I am having problems with my QSG301 quartz monitor. Does anyone out there have an old system for parts, specifically the transducer and replacement quartz crystals, or schematics for it?
Look forward to hearing from someone in this regard.
Steve -- Dr. Steven Barlow EM Facility/Biology Dept. San Diego State University 5500 Campanile Drive San Diego CA 92182-4614 phone: (619) 594-4523 fax: (619) 594-5676
Coincidentally, I just finished a study of tension wood in sweetgum. I did immunogold-silver and didn't take it the TEM, but the sections looked pretty good. Because we wanted optimal immunoreactivity, we used LR white (not epon). Here's what I did:
Using a chisel, a wafer of wood about 1x1 cm square (parallel to the surface of the trunk) and about 3-5 mm thick was removed from a living tree. The wafer was immediately immersed in water and split into "matchsticks", approximately 2-3mm per side, by inserting a razor ~1 mm into the top edge of the wafer and splitting it along the grain (not cutting). These "matchsticks" were then put into gluteraldehyde and, using a brand-new razor blade, cut into 1-2mm thick slices. So you have squares of wood, about the size of a TEM grid, about 1-2mm thick, with the long edge of the cells running top to bottom through the thinnest dimension. By splitting the wood (and not just cutting it), you are assured that the orientation of the tracheids are parallel to the long axis of the matchstick. So when you cut the matchsticks into wafers, the trachids are wide open to your solutions, thus facilitating the infiltration of the wood specimen. The rational here is that these cells were conducting water before you removed them from the tree, they should conduct alcohol and resin, too. The samples were fixed for 24 hours at room temp and then dehydrated in 25%, 50%, 75% (2 hours each) and absolute ethanol overnight. They were then infiltrated with LR white resin with increasing concentrations (25%, 50%, 75%, neat - 24 hours each step), also at room temperature. Specimens in neat resin were placed onto a shaking platform for 48 hours. Slices were placed into cylindrical polyethylene capsules and oriented with the faces of the wafers facing the bottom of the capsule and polymerized at 55º C for 2 hours. We cut mostly 0.55 micron sections.
Other notes: you'll want to be really slow with the block trimming - this resin-wood material is extremely tough. Take really thin slices with a new blade. These blocks are also really hard on the diamond knife.
I'll send you a picture to back up my protocol :)
Andy Bowling
p.s. My sweetgum samples didn't require this, but if your wood is really dry (i.e. it is full of air, won't sink in your solutions, etc.), you might have to vacuum it to get the air out and your solutions in. I'd vacuum the wafers - the air has the smallest distance to travel.
p.p.s. I got this "matchstick" technique from Clair et al, 2005: Precautions for the structural analysis of the gelatinous layer in tension wood. IAWA Journal 26: 189-195.
-----Original Message----- X-from: heller-at-uni-hohenheim.de [mailto:heller-at-uni-hohenheim.de] Sent: Monday, December 03, 2007 1:18 PM To: Bowling, Andrew
Hi to all,
I tried to prepare wood (Fagus and Pinus)for transmission electron microscopy by embedding in Epon and LR-White and got terrible problems in sectioning. The blocks seemed ok, they were not soft. We got sections, but they immersed in the trough and did not stretch. The result was aweful. I do not understand what went wrong, maybe, poor infiltration? Is there anybody who has experience with wooden material?
Best regards, Anne
==============================Original Headers============================== 4, 30 -- From heller-at-uni-hohenheim.de Mon Dec 3 13:13:15 2007 4, 30 -- Received: from smtp1.rz.uni-hohenheim.de (smtp1.rz.uni-hohenheim.de [144.41.4.41]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB3JDF8N015553 4, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 13:13:15 -0600 4, 30 -- Received: from localhost (smtp2.rz.uni-hohenheim.de [144.41.4.42]) 4, 30 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id B0C7F11D3A7 4, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 20:13:13 +0100 (CET) 4, 30 -- X-Virus-Scanned: amavisd-new at uni-hohenheim.de 4, 30 -- Received: from smtp1.rz.uni-hohenheim.de ([144.41.4.41]) 4, 30 -- by localhost (webmail3.rz.uni-hohenheim.de [144.41.4.42]) (amavisd-new, port 10024) 4, 30 -- with ESMTP id 7y28ue8YeTG0 for {Microscopy-at-microscopy.com} ; 4, 30 -- Mon, 3 Dec 2007 20:13:06 +0100 (CET) 4, 30 -- Received: from webmail.uni-hohenheim.de (webmail2.rz.uni-hohenheim.de [144.41.4.31]) 4, 30 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 6EDB611D3D8 4, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 20:13:05 +0100 (CET) 4, 30 -- Received: by webmail.uni-hohenheim.de (Postfix, from userid 48) 4, 30 -- id B726C60153; Mon, 3 Dec 2007 20:13:04 +0100 (CET) 4, 30 -- Received: from jatropha.botanik.uni-hohenheim.de (jatropha.botanik.uni-hohenheim.de [144.41.33.113]) 4, 30 -- by webmail.uni-hohenheim.de (IMP) with HTTP 4, 30 -- for {heller-at-10.0.0.5} ; Mon, 3 Dec 2007 20:13:04 +0100 4, 30 -- Message-ID: {1196709184.475455409f6cb-at-webmail.uni-hohenheim.de} 4, 30 -- Date: Mon, 3 Dec 2007 20:13:04 +0100 4, 30 -- From: heller-at-uni-hohenheim.de 4, 30 -- To: Microscopy-at-microscopy.com 4, 30 -- Subject: preparation of wood for TEM 4, 30 -- MIME-Version: 1.0 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 4, 30 -- Content-Transfer-Encoding: 8bit 4, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2.2 4, 30 -- X-Originating-IP: 144.41.33.113 ==============================End of - Headers==============================
==============================Original Headers============================== 19, 31 -- From Andrew.Bowling-at-ARS.USDA.GOV Mon Dec 3 14:45:04 2007 19, 31 -- Received: from messagescreen3.ars.usda.gov (messagescreen3.ars.usda.gov [199.133.180.150]) 19, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB3Kj3b9010640 19, 31 -- for {microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 14:45:04 -0600 19, 31 -- Received: from CO-MAILBH-02.ARSNET.ARS.USDA.GOV ([199.133.183.227]) 19, 31 -- by messagescreen3.ars.usda.gov (8.13.8/8.13.8) with ESMTP id lB3KiLwZ011193 19, 31 -- for {microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 14:45:04 -0600 19, 31 -- Received: from CO-MAIL-03.ARSNET.ARS.USDA.GOV ([10.100.2.202]) by CO-MAILBH-02.ARSNET.ARS.USDA.GOV with Microsoft SMTPSVC(6.0.3790.3959); 19, 31 -- Mon, 3 Dec 2007 13:39:43 -0700 19, 31 -- x-mimeole: Produced By Microsoft Exchange V6.5 19, 31 -- Content-class: urn:content-classes:message 19, 31 -- MIME-Version: 1.0 19, 31 -- Content-Type: text/plain; 19, 31 -- charset="iso-8859-1" 19, 31 -- Subject: [Microscopy] Re: preparation of wood for TEM 19, 31 -- Date: Mon, 3 Dec 2007 13:36:20 -0700 19, 31 -- Message-ID: {8017F94146BF634DA9414E4B9088525B6E7D29-at-CO-MAIL-03.ARSNET.ARS.USDA.GOV} 19, 31 -- In-Reply-To: {200712031918.lB3JI4G4025898-at-ns.microscopy.com} 19, 31 -- X-MS-Has-Attach: 19, 31 -- X-MS-TNEF-Correlator: 19, 31 -- Thread-Topic: [Microscopy] Re: preparation of wood for TEM 19, 31 -- Thread-Index: Acg14TwE463nMNEgRWCTkH1J1sniuQABPWAQ 19, 31 -- From: "Bowling, Andrew" {Andrew.Bowling-at-ARS.USDA.GOV} 19, 31 -- To: {microscopy-at-microscopy.com} 19, 31 -- X-OriginalArrivalTime: 03 Dec 2007 20:39:43.0302 (UTC) FILETIME=[A2632660:01C835EC] 19, 31 -- X-MessageScreenMessageID: 1196714704.251248.1253.22691873 19, 31 -- X-MessageScreenContentScore: Score of 0 assigned to Content 19, 31 -- X-MessageScreenUCEScore: Score of 0 assigned to UCE 19, 31 -- X-MessageScreen: Analyzed by IntelliReach MessageScreen(tm) 19, 31 -- Content-Transfer-Encoding: 8bit 19, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lB3Kj3b9010640 ==============================End of - Headers==============================
I'm posting this advert in my role as a researcher at the University of Cambridge, UK. Please send any replies to James Loudon or Paul Midgley, as described below.
Richard Beanland
Post-Doctoral Research Associate
IMAGING THE STRUCTURE AND DYNAMICS OF FLUX VORTICES IN HIGH TEMPERATURE SUPERCONDUCTORS
DEPARTMENT OF MATERIALS SCIENCE AND METALLURGY
Applications are invited for a postdoctoral research position, funded by the EPSRC, to investigate the structure and dynamics of flux vortices in high Tc superconductors, using low- temperature transmission electron microscopy (TEM). The successful applicant will work closely with Dr James Loudon and Prof Paul Midgley in the Electron Microscopy Group and will use Lorentz microscopy and electron holography to image individual vortices, vortex ordering and dynamics, and the control of flux vortices. The project will focus in particular on the behaviour of fluxons in confined mesoscale geometries in samples prepared using a Dual Beam workstation. The research will form part of ongoing collaborations with the Device Materials Group in the Department, and with groups at the Cavendish Laboratory and the University of Manchester.
Applicants should have (or be about to receive) a PhD and experience in transmission electron microscopy is essential. Knowledge of magnetic imaging and superconductivity would be an advantage. Post-doctoral appointments will be for up to 3 years and the salary will be at grade 7 on the university salary scale (currently £25,134 to £32,796). The post is available immediately.
Informal enquiries can be made to Dr James Loudon e-mail: j.c.loudon-at-gmail.com or Prof Paul Midgley, e-mail: pam33-at-cam.ac.uk
Applications should be emailed to j.c.loudon-at-gmail.com or sent by post to Dr J.C. Loudon, Department of Materials Science and Metallurgy, University of Cambridge, Pembroke Street, Cambridge, CB2 3QZ, UK, and should include a covering letter, CV, publication list, the names and addresses of two referees and a PD18 form with parts I and III completed (downloadable from http://www.admin.cam.ac.uk/offices/personnel/forms/pd18/).
Further details can be obtained from: http://www-hrem.msm.cam.ac.uk/vacancies/
Closing Date for applications: 31 December 2007
==============================Original Headers============================== 16, 25 -- From contact-at-integrityscientific.com Mon Dec 3 15:18:41 2007 16, 25 -- Received: from em-p07-ob.rzone.de (em-p07-ob.rzone.de [81.169.146.246]) 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB3LIflR023738 16, 25 -- for {microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 15:18:41 -0600 16, 25 -- Received: from post.webmailer.de (teg.store [192.168.40.117]) 16, 25 -- by mad-em-02.store (RZmta 14.0) with ESMTP id J001ffjB3I0cjh 16, 25 -- for {microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 22:18:40 +0100 (MET) 16, 25 -- (envelope-from: {contact-at-integrityscientific.com} ) 16, 25 -- Received: (from httpd-at-localhost) 16, 25 -- by post.webmailer.de (8.13.6/8.13.6) id lB3LIecs013175 16, 25 -- for microscopy-at-microscopy.com; Mon, 3 Dec 2007 22:18:40 +0100 (MET) 16, 25 -- Date: Mon, 3 Dec 2007 22:18:40 +0100 (MET) 16, 25 -- Message-Id: {200712032118.lB3LIecs013175-at-post.webmailer.de} 16, 25 -- X-Authentication-Warning: teg.store: httpd set sender to contact-at-integrityscientific.com using -f 16, 25 -- To: microscopy-at-microscopy.com 16, 25 -- From: "Richard Beanland" {contact-at-integrityscientific.com} 16, 25 -- Subject: Job posting - TEM 16, 25 -- X-Priority: 3 16, 25 -- X-Abuse: 982623 / 131.111.102.43 16, 25 -- X-RZG-MBID: 15cBjAKTcdbsEQ== 16, 25 -- MIME-Version: 1.0 16, 25 -- Content-Type: text/plain; charset="ISO-8859-1" 16, 25 -- X-RZG-CLASS-ID: em07 16, 25 -- Content-Transfer-Encoding: 8bit 16, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lB3LIflR023738 ==============================End of - Headers==============================
The Microbeam Analysis Society is organizing a topical workshop on EBSD, May 20-21-22, 2008, at the University of Wisconsin-Madison.
The first day will be an all day tutorial for beginners, on the basics of EBSD techniques and application. The instructors will be Andrew Deal (General Electric Global Research Center) and Joseph Michael (Sandia National Laboratories). Specific topics to be addressed include: Forming, collecting, and indexing EBSD patterns in the SEM; Using EBSD to measure microtexture in materials; Identification of crystalline phases using EBSD and EDX; Application of EBSD to Material Science and Geological problems. Later in the afternoon there will be an "Ask the experts" session, with experienced practitioners of EBSD to answer questions about its practical use: J.R. Michael, L.N. Brewer, S.I. Wright (EDAX), S. Sitzman (Oxford), A. Deal.
The next two days of the workshop will consist of invited and contributed talks, with general categories including: In situ measurements in EBSD; EBSD in three dimensions; Frontiers in EBSD technique development; Materials Science and Engineering applications; Geological applications.
Confirmed speakers and titles of their talks are: David Prior, University of Liverpool - "The beauty of triclinics: advantages and challenges of EBSD studies of plagioclase feldspar"; Hans-Rudolf Wenk, University of California at Berkeley - "EBSD for texture analysis: Quantification, limitations and comparison with other techniques"; Donna Whitney, University of Minnesota - "Applications of EBSD to metamorphic petrology: the tectonics of metamorphic crystallization"; Aimo Winkelmann, MPI für Mikrostrukturphysik - "Recent Advances in Dynamical Simulations of EBSD"; Gregory Rohrer, Carnegie Mellon University - "Determining Five-Parameter Grain Boundary Character Distributions From Orientation Mapping in Three Dimensions"; David Rowenhurst, Naval Research Laboratory - "Crystallographic and Morphological Analysis by Combining EBSD and Serial Sectioning".
Thanks to support from NSF and others, there will be a significant amount of funds for student support.
There will be limited enrollment, so early registration is urged. Online registration will commence January 15 at www.microbeamanalysis.org
Contact John Fournelle for more information johnf-at-geology.wisc.edu
Sponsored by: MAS, NSF, EDAX-TSL, and Oxford-HKL, with additional support from Hitachi-USA and JEOL-USA. -- ======================================================== John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480 Cameron Electron Microprobe Lab lab: (608) 265-4798 Dept of Geology & Geophysics fax: (608) 262-0693 University of Wisconsin home: (608) 274-2245 1215 West Dayton St. email: johnf-at-geology.wisc.edu Madison, WI 53706 amateur radio: WA3BTA Personal http://www.geology.wisc.edu/~johnf/ Probe lab http://www.geology.wisc.edu/~johnf/sx51.html Probe Sign Up Calender: http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi
"The first rule of all intelligent tinkering is to save every cog and wheel." -- Aldo Leopold
"For a successful technology, reality must take precedence over public relations, for Nature cannot be fooled." -- Richard P. Feynman
==============================Original Headers============================== 11, 26 -- From johnf-at-geology.wisc.edu Mon Dec 3 15:19:14 2007 11, 26 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB3LJEjm024756 11, 26 -- for {microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 15:19:14 -0600 11, 26 -- Received: from localhost (localhost [127.0.0.1]) 11, 26 -- by localhost (Postfix) with ESMTP id 58D4E20D10 11, 26 -- for {microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 15:19:14 -0600 (CST) 11, 26 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) 11, 26 -- by localhost (geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 11, 26 -- with ESMTP id 15636-01 for {microscopy-at-microscopy.com} ; 11, 26 -- Mon, 3 Dec 2007 15:18:50 -0600 (CST) 11, 26 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57]) 11, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 11, 26 -- (No client certificate requested) 11, 26 -- by ice.geology.wisc.edu (Postfix) with ESMTP id 8FEBF20D04 11, 26 -- for {microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 15:05:32 -0600 (CST) 11, 26 -- Mime-Version: 1.0 11, 26 -- Message-Id: {p0623090bc37a1fbd45ff-at-[144.92.206.57]} 11, 26 -- Date: Mon, 3 Dec 2007 15:05:28 -0600 11, 26 -- To: microscopy-at-microscopy.com 11, 26 -- From: John Fournelle {johnf-at-geology.wisc.edu} 11, 26 -- Subject: EBSD Workshop May 2008 Madison WI 11, 26 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 11, 26 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu 11, 26 -- Content-Transfer-Encoding: 8bit 11, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lB3LJEjm024756 ==============================End of - Headers==============================
Brian- TA fix is likely one very promising option, as Stephane and Brian suggest; epoxy for detail, as David suggests. For detergent-glycerol-demembranated muscle fibers, we have often used TA as a primary fix as well, followed by UA or OsO4, with consistently pleasing results --- works in aqueous physiological buffers, or in acetone when doing freeze-substitution. At 0.2%, combined with 1-3% glutaraldehyde, it can usually permeate single cells (thus, likely spermatozoa) to enhance fixation quality and contrast as well. Don't combine TA with PVP, TX-100, vanadate or PO4 (at greater than 10 mM); it will form a complex, may precipitate, becomes unavailable for fixation. However, a promising protocol is that of Maupin & Pollard (1983; J Cell Biol 96:51-62) who combined Glut-TA with saponin to help TA penetrate membranes, and introduced an important modification of OsO4 post-fix procedure that we have used ever since whenever using OsO4.
We don't recommend Epon because section staining with KMnO4 --} Sato's lead for maximum contrast shows finest granularity in Araldite. If using Epon, avoid NMA, use DDSA only if Mn-Pb stain is to be tried.
Mallinckrodt #1764 seemed the best TA in the 1980s, after the Simionescus in Palade's lab called attention to it in 1976 (J Cell Biol 70:608 and 70:622). To us, its behavior seemed to become inconsistent in the early 1990s, so we switched to the EMS products. Currently, the #21700 low MW (FW 1000-1500) works well. I'd guess the #21710 EM grade (FW 1701.28) probably works equally well for most specimens-- it is the same MW spec as the Mallinckrodt 1764.
Usual disclaimers-- we have no connection wIth EMS except as satisfied users.
-mike reedy-
At 12:52 PM -0600 11/29/07, bharris-at-uoguelph.ca wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
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Email: mbisher-at-princeton.edu Name: Margaret Bisher
Organization: Princeton University
Title-Subject: [Filtered] Job Opening
Question: Specialist in the PRISM Imaging and Analysis Center at Princeton University
Applications are invited from qualified candidates for a technical specialist position in the Imaging and Analysis Center (www.princeton.edu/~iac), an integral part of the Princeton Institute for the Science and Technology of Materials, at Princeton University.
Note: The specialist level will be determined based on experience and educational level.
Specific duties include: ï Instruct and assist students and researchers in the operation of state-of- the-art instruments for imaging (optical, electron and scanning probe microscopes), and analysis (XRD, EDX, WDX) ï Support centerís daily operation, responsible for maintenance of microscopes, electronics, vacuum and chilling systems ï Provide expertise in SEM and TEM sample preparation for both hard and soft materials ï Support the teaching programs and perform other tasks as required
Essential Qualifications: ï Masterís degree in physical science and engineering and /or 5+ years related work experience is required ï Competent skills in sample preparation techniques, including ion milling, ultramicrotome, staining, coating and polishing samples ï Good knowledge on electron microprobe analysis is preferred ï Excellent communication and interpersonal skills are essential
Start date is January 1, 2008 or as soon as possible. Interested candidates should send application online at https://jobs.princeton.edu. (requisition # 0700851)
Princeton University is an Equal Opportunity/Affirmative Action Employer. For general application information and how to voluntarily self-identify, please link to
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Question: We have a 1993 Personal SEM v2.0 made by RJ Lee. We need a new motherboard for a 486 DX2 computer that runs Windows 3.1, in order to get the PSEM running. Does anyone know where we can find one?
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Position Summary: The Application Scientist is a critical role providing application support to customers utilizing SIMS and EPMA technology. This is a high visibility position requiring an advanced scientist with expertise in materials and x-ray techniques. Extensive travel will be necessary.
Responsibilities: * Support existing applications for key customers * Develop new applications (second stage) * Teach customers Best Known Methods and techniques * Prepare presentations for conference calls and meetings * Write reports to provide insights to sales and technical staff * Contribute to training materials and conduct customer training sessions * Attend conferences and trade shows
Required Qualifications:
* MS with 2 years of experience in Applied Physics, Physical Chemistry or Materials Science Engineering * Good organization skills * Solid understanding of electron-beam, ion-beam and x-ray beam interactions with matter * Ability to learn new concepts and techniques quickly * Strong analytical problem solving skills * Excellent internal and external communication skills
Preferred Qualifications:
* Experience in the semiconductor fabrication industry (clean room), or equivalent experience and education * PhD preferred * WDS and/or EDS knowledge * Understanding of LEXES technique * Previous trouble shooting experience * Understanding of thin film processing
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Email: mlibbee-at-gmail.com Name: Marissa
Title-Subject: [Filtered] SEM Imaging of CNT
Question: Good Afternoon!
I need to know if CNT imaging (thickness measurement) can be performed without coating the sample with AuPd? If so, what are the parameters/conditions required of the microscope? I currently use a Hitachi 4700 SEM.
Also, does anyone suggest a specific amount for the AuPd coating on the CNT? Is there an advantage to breaking the wafer under LN2 temperature or coating the wafer prior to cleaving the area of interest?
Thank you for your time and continued support, Marissa
That would be rare to find these days. Does the board need many ISA slots? Does the PC have SEM-specific plug in board?
In some computer stores, they have older MBs laying around. I would suggest that you try to locate an early Pentium board and CPU and run Win98SE. Apps for 3.1 should work on Win98SE.
gary g.
At 04:50 PM 12/3/2007, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Mon Dec 3 19:08:50 2007 10, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lB418orR017193 10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 19:08:50 -0600 10, 20 -- Message-Id: {200712040108.lB418orR017193-at-ns.microscopy.com} 10, 20 -- Received: (qmail 14552 invoked from network); 3 Dec 2007 17:08:49 -0800 10, 20 -- Received: by simscan 1.1.0 ppid: 14549, pid: 14550, t: 0.0778s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp1 with SMTP; 3 Dec 2007 17:08:49 -0800 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Mon, 03 Dec 2007 17:08:44 -0800 10, 20 -- To: llawres-at-mail.usf.edu 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] viaWWW: RJ Lee PSEM 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200712040050.lB40orWs011586-at-ns.microscopy.com} 10, 20 -- References: {200712040050.lB40orWs011586-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-19CC7E99 ==============================End of - Headers==============================
The Personal SEM business was spun off to a company in Delmont, PA as Aspex Corp. That is my understanding. I would contact them and I sent you a personal email address of the best person I know to contact there. I am sure he can help point you to the proper person if he can't help you.
HTH,
Paul
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==============================Original Headers============================== 5, 28 -- From beaurega-at-westol.com Mon Dec 3 21:39:44 2007 5, 28 -- Received: from smtp-gateway-5.winbeam.com (smtp-gateway-5.winbeam.com [64.84.97.70]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB43dhQu001932 5, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 21:39:44 -0600 5, 28 -- X-Winbeam-MailScanner-Watermark: 1197342407.03952-at-/kw3y+HF0DYJx587k+NNOA 5, 28 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 5, 28 -- by smtp-gateway-5.winbeam.com (8.13.2/8.12.8) with SMTP id lB4352He028728 5, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 22:05:42 -0500 5, 28 -- Received: (qmail 31762 invoked by uid 89); 4 Dec 2007 03:04:52 -0000 5, 28 -- Received: from pitts-69-72-117-21.dynamic-dialup.coretel.net (HELO millenium) (69.72.117.21) 5, 28 -- by mail.winbeam.com with SMTP; 4 Dec 2007 03:04:52 -0000 5, 28 -- Message-Id: {3.0.6.32.20071203003449.007fd8f0-at-pop3.norton.antivirus} 5, 28 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus 5, 28 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 5, 28 -- Date: Mon, 03 Dec 2007 00:34:49 -0500 5, 28 -- To: llawres-at-mail.usf.edu, microscopy-at-microscopy.com 5, 28 -- From: Beaurega {beaurega-at-westol.com} 5, 28 -- Subject: Re: [Microscopy] viaWWW: RJ Lee PSEM 5, 28 -- In-Reply-To: {200712040047.lB40lnWN002745-at-ns.microscopy.com} 5, 28 -- Mime-Version: 1.0 5, 28 -- Content-Type: text/plain; charset="us-ascii" 5, 28 -- X--MailScanner-Information: - Please contact Technical Support for more information 5, 28 -- X--MailScanner: Found to be clean (courtesy of MailScanner) 5, 28 -- X--MailScanner-SpamCheck: not spam (whitelisted), SpamAssassin (not cached, 5, 28 -- score=-4.889, required 4, autolearn=not spam, AWL -0.38, 5, 28 -- BAYES_00 -0.50, DATE_IN_PAST_12_24 0.99, local_FROM_WB -1.00, 5, 28 -- local_HAM_FROM_WB -4.00) 5, 28 -- X--MailScanner-From: beaurega-at-westol.com ==============================End of - Headers==============================
We look very offen at CNT by SEM (with a Jeol6700F, an equivalent to the S4700), and we never coat the samples. We have organised growth of CNT, (carpet or lawn grass like), on Si coated with a 5-10 layer of SiO2 or TiN (both insultor), and at low voltage (less than 3 keV) and short working distance (less than 5 mm), it works well. The only limitation is a little bit drift at high mag (more than x50000), when the insulator is thicker.
I mount the sample most on a home made 45° or 60° pré-tilted holder, to see them with some angle, without the need to use the stage tilt. It allowed to have a tilted view at very short WD (45° or more at 1.5-2 mm). The samples are in most cases not cleaved and only scratched with a diamant tip, to make some "desorder" in the CNT coating. Depens on what we want to see. If needed we cleved them at room temperature.
Observations are most made with the in lens detector. Sometimes we use a higher beam energy to visualise the catalyser, inside the NTs.
One of my collegue observes sometimes CNT grown on ceramics, with catalysts, and he use only carbon coating, and the same observation conditions as me.
I would avoid to metallize the sample as far as possible, and play with the primary energy, WD and detector.
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
mlibbee-at-gmail.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mlibbee-at-gmail.com } Name: Marissa } } Title-Subject: [Filtered] SEM Imaging of CNT } } Question: Good Afternoon! } } I need to know if CNT imaging (thickness measurement) can be performed without coating the sample with AuPd? If so, what are the parameters/conditions required of the microscope? I currently use a Hitachi 4700 SEM. } } Also, does anyone suggest a specific amount for the AuPd coating on the CNT? Is there an advantage to breaking the wafer under LN2 temperature or coating the wafer prior to cleaving the area of interest? } } Thank you for your time and continued support, } Marissa } } Marissa } } Login Host: 63.163.107.100 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Mon Dec 3 18:48:24 2007 } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB40mMVp004721 } 9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 3 Dec 2007 18:48:23 -0600 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240803c37a543b7061-at-[206.69.208.22]} } 9, 11 -- Date: Mon, 3 Dec 2007 18:48:22 -0600 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: mlibbee-at-gmail.com (by way of MicroscopyListserver) } 9, 11 -- Subject: viaWWW: SEM Imaging of CNT } 9, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 12, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Dec 4 07:39:38 2007 12, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.156]) 12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB4Ddb1D030193 12, 29 -- for {microscopy-at-microscopy.com} ; Tue, 4 Dec 2007 07:39:38 -0600 12, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 12, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id lB4DdXP2007883 12, 29 -- for {microscopy-at-microscopy.com} ; Tue, 4 Dec 2007 14:39:33 +0100 (CET) 12, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 12, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 55DC4BE43FA 12, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 4 Dec 2007 14:37:00 +0100 (CET) 12, 29 -- Message-ID: {47555804.6020701-at-ipcms.u-strasbg.fr} 12, 29 -- Date: Tue, 04 Dec 2007 14:37:08 +0100 12, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 12, 29 -- User-Agent: Thunderbird 1.5.0.14pre (X11/20071022) 12, 29 -- MIME-Version: 1.0 12, 29 -- To: Microscopy-at-microscopy.com 12, 29 -- Subject: Re: [Microscopy] viaWWW: SEM Imaging of CNT 12, 29 -- References: {200712040058.lB40wj3W002100-at-ns.microscopy.com} 12, 29 -- In-Reply-To: {200712040058.lB40wj3W002100-at-ns.microscopy.com} 12, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 29 -- Content-Transfer-Encoding: 8bit 12, 29 -- X-IPCMS-MailScanner: Found to be clean 12, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 12, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.156]); Tue, 04 Dec 2007 14:39:34 +0100 (CET) 12, 29 -- X-Virus-Scanned: ClamAV 0.88.7/4992/Tue Dec 4 12:21:35 2007 on mr6.u-strasbg.fr 12, 29 -- X-Virus-Status: Clean 12, 29 -- X-Spam-Status: No, score=-0.0 required=5.0 tests=AWL autolearn=disabled 12, 29 -- version=3.1.8 12, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr6.u-strasbg.fr ==============================End of - Headers==============================
We have a couple of these units, and I have all documentation if you need a copy. We recently got crystals from Tangidyne http://www.tangidyne.com/; very good pricing and excellent performance - much less temperature effect on the "RC" (TAN05RCG) - email them - they'll get you what you need. You probably just need new crystals if the unit has been kicking around and dirty. The specs show the circuit and how to test and adjust the driver, etc. Let me know.
Dale Callaham Umass Amherst
sbarlow-at-sunstroke.sdsu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello All } } I am resurrecting a Balzers 301 freeze fracture unit. The coating } and vacuum units seem to be working ok. I am having problems with my } QSG301 quartz monitor. Does anyone out there have an old system for } parts, specifically the transducer and replacement quartz crystals, } or schematics for it? } } Look forward to hearing from someone in this regard. } } Steve
==============================Original Headers============================== 4, 22 -- From dac-at-research.umass.edu Tue Dec 4 09:07:13 2007 4, 22 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB4F7DDA012704 4, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Dec 2007 09:07:13 -0600 4, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 4, 22 -- (authenticated bits=0) 4, 22 -- by race3.oit.umass.edu (8.14.1/8.14.1) with ESMTP id lB4F7C9I001615 4, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 4, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Dec 2007 10:07:13 -0500 4, 22 -- Message-ID: {47556D7B.1040101-at-research.umass.edu} 4, 22 -- Date: Tue, 04 Dec 2007 10:08:43 -0500 4, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 4, 22 -- Reply-To: dac-at-research.umass.edu 4, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.9) Gecko/20071030 SeaMonkey/1.1.6 4, 22 -- MIME-Version: 1.0 4, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 22 -- Subject: Re: [Microscopy] Balzers freeze fracture quartz monitor 4, 22 -- References: {200712031922.lB3JMwaj004431-at-ns.microscopy.com} 4, 22 -- In-Reply-To: {200712031922.lB3JMwaj004431-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
On Nov 27, 2007, at 8:37 AM, g-leser-at-northwestern.edu wrote:
} We find ourselves with some extra money and I would like to get a } table top carbon evaporator. Do these exist? Can anyone make any } recommendations? It would be used for the basics, namely carbon } coating grids, preparing carbon films, and glow discharging grids. } Any help, commercial and non, would be greatly appreciated.
On Nov 28, 2007, at 7:02 PM, dsherman-at-purdue.edu wrote: } I was told that the best way to clean SEM aperture strips is with a } plasma } cleaner. Can any one suggest an inexpensive one...if there is such } a thing. }
Dear George and Debby, We have been very happy with our Cressington 208 for carbon (or metal) evaporation and with our Harrick plasma cleaner for cleaning and glow discharging. The cost of the Harrick unit was ~$2500, and we bought a mechanical vacuum pump to go with it for about the same price. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 9, 22 -- From tivol-at-caltech.edu Tue Dec 4 12:27:21 2007 9, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB4IRKLb030542 9, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 4 Dec 2007 12:27:20 -0600 9, 22 -- Received: from fire-dog.its.caltech.edu (fire-dog [192.168.1.4]) 9, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id 1C5BC13992 9, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 4 Dec 2007 10:27:18 -0800 (PST) 9, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 9, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id 9433B13EF5 9, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 4 Dec 2007 10:27:16 -0800 (PST) 9, 22 -- Mime-Version: 1.0 (Apple Message framework v752.3) 9, 22 -- In-Reply-To: {200711271637.lARGbhC4024200-at-ns.microscopy.com} 9, 22 -- References: {200711271637.lARGbhC4024200-at-ns.microscopy.com} 9, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 9, 22 -- Message-Id: {A81191D5-D306-4285-A870-24D362946395-at-caltech.edu} 9, 22 -- Content-Transfer-Encoding: 7bit 9, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 9, 22 -- Subject: Re: [Microscopy] tabletop carbon evaporator 9, 22 -- Date: Tue, 4 Dec 2007 10:27:25 -0800 9, 22 -- To: microscopy-at-msa.microscopy.com 9, 22 -- X-Mailer: Apple Mail (2.752.3) 9, 22 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.4.5 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pgan-at-ap.ansell.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, December 4, 2007 at 03:22:23 ---------------------------------------------------------------------------
Email: pgan-at-ap.ansell.com Name: Phay Fang Gan
Organization: Ansell Shah Alam sdn Bhd
Education: Graduate College
Location: Shah Alam, Selangor, Malaysia
Question: Does anyone know the rough temperature of the electron beam when reaching the surface of the spcimen ?
I'm thinking that the temperature of the electrons in a beam should be determined from E=3/2 kT. Would there be more than 3 degrees of freedom in an electron beam? I'm not sure if we should consider rotational freedoms or not.
Now if you are asking about the temperature of the sample, that is another question. It depends on the beam energy, current density, and materials properties and dimensions, i.e. thickness.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: pgan-at-ap.ansell.com [mailto:pgan-at-ap.ansell.com] Sent: Wednesday, December 05, 2007 6:22 AM To: Walck-at-SouthBayTech.com
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pgan-at-ap.ansell.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, December 4, 2007 at 03:22:23 ---------------------------------------------------------------------------
Email: pgan-at-ap.ansell.com Name: Phay Fang Gan
Organization: Ansell Shah Alam sdn Bhd
Education: Graduate College
Location: Shah Alam, Selangor, Malaysia
Question: Does anyone know the rough temperature of the electron beam when reaching the surface of the spcimen ?
Lockheed Martin- KAPL, Inc. has an open position for a materials scientist/engineer/electron microscopist to provide SEM materials testing/failure analysis support. A brief description of the job is given below. NOTE: the position requires US Citizenship.
The complete job announcement (Req ID 60318BR) can be found at the Lockheed Martin career web site (LockheedMartin.com).
Jim McGee ************************************ James J. McGee Lockheed Martin, KAPL, Inc. PO Box 1072 Schenectady, NY 12301-1072
OPEN POSITION - Lockheed Martin, KAPL, Inc., Schenectady, NY
Req ID 60318BR Industry Job Title Materials Engineer Standard Job Code/Title E1862:Materials Engrg Required skills BS degree in the physical sciences (e.g., materials, chemistry, geology, solid state physics) or engineering plus hands-on training and/or experience utilizing scanning electron microscopy and X-ray microanalysis for materials characterization, development or failure analysis. Desired skills MS or PhD degree in physical sciences, with 5 years or more experience in scanning electron microscopy and microanalysis (energy dispersive spectrometry, electron backscatter diffraction), and demonstrated problem solving experience in areas of metallurgy, alloy testing/development, solid inorganic materials, and/or ceramics. Experience with other associated techniques, especially Focused Ion Beam (FIB) and Transmission Electron Microscope (TEM) utilization and application. Previous work with nuclear or radioactive materials, fractographic analysis, corrosion, and/or mechanical metallurgy. Specific Job Description Scanning electron microscopy (SEM) characterization of materials to evaluate response of materials to environmental testing and failure analysis of in-service components. Microstructural and microchemical characterization utilizing field emission SEM imaging, energy dispersive spectroscopy (EDS) and electron backscatter diffraction (EBSD) techniques. Data and image processing, data assimilation, and reporting of results. Individual would perform multidisciplinary collaborative investigations and team-oriented problem solving with other materials characterization specialists and materials scientists/engineers integrating use of associated analytical techniques (EPMA, XRD, Auger, XPS, TEM). Utilize skills in high resolution imaging, microchemical analysis, and ability to evaluate and recommend best sample preparation and characterization methodologies for problems.
The major duties will be use of advanced scanning electron microscopy and associated analytical techniques to determine the microstructure and microchemistry of metals, alloys, and ceramic materials. Ensure instrumentation is operating in proper condition; measure structure and composition of materials using SEM, EDS, WDS, EBSD, etc. Analyze and interpret data and communicate results to customers and sponsoring groups by oral and written reports. Serve as part of research teams to design experiments that will elucidate structure-composition-property-processing relationships. Stay current in microscopic and microanalytical techniques and applications. Propose and implement new capabilities or characterization tools.
Applicants selected will be subject to a Federal background investigation and must meet eligibility requirements for access to classified matter. US citizenship is required.
==============================Original Headers============================== 9, 26 -- From mcgeejj-at-kapl.gov Wed Dec 5 16:59:54 2007 9, 26 -- Received: from kaplmail.kapl.gov (kaplmail.kapl.gov [149.37.1.125]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB5MxrDL024700 9, 26 -- for {microscopy-at-microscopy.com} ; Wed, 5 Dec 2007 16:59:53 -0600 9, 26 -- Received: from KIASFW01.internet.kapl.gov ([192.168.200.120]) by INETAV2 9, 26 -- with InterScan Messaging Security Suite; Wed, 05 Dec 2007 18:03:08 -0500 9, 26 -- Received: from ([10.1.67.209]) by KIASFW01.internet.kapl.gov; Wed, 05 Dec 9, 26 -- 2007 18:14:28 -0500 (EST) 9, 26 -- Message-ID: {017001c83792$8ab77190$d143010a-at-kias.kapl.gov} 9, 26 -- From: "J.J. McGee" {mcgeejj-at-kapl.gov} 9, 26 -- To: {microscopy-at-microscopy.com} 9, 26 -- Subject: Open Position - SEM microscopist for materials science applications 9, 26 -- Date: Wed, 5 Dec 2007 17:59:50 -0500 9, 26 -- MIME-Version: 1.0 9, 26 -- Content-Type: text/plain; 9, 26 -- format=flowed; 9, 26 -- charset=iso-8859-1; 9, 26 -- reply-type=response 9, 26 -- Content-Transfer-Encoding: 7bit 9, 26 -- X-Priority: 3 9, 26 -- X-MSMail-Priority: Normal 9, 26 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 9, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 9, 26 -- X-imss-version: 2.049 9, 26 -- X-imss-result: Passed 9, 26 -- X-imss-approveListMatch: *-at-kapl.gov ==============================End of - Headers==============================
I need to determine the average refractive index of a powder of an organic compound. I would use light microscopy to do this, but the particle size is very small and the material is slightly soluble in the refractive index liquids I have access to. I'd like to check my observations against a calculated value. So... is there anyone out there who has used the Gladstone-Dale relationship (avg. refractive index = 1 + specific refractive energy x density) to calculate the refractive index of organic crystals? I would like to be able to calculate an average refractive index of a material given it's composition and density (both of which I can get). There is a significant amount of work on minerals using this relationship, but I haven't found many references on organics, particularly on specific refractive energies of organic compounds. I'm sure there is someone out there who knows more about this than myself.
Thanks in advance for your help! Bryan Bandli
-- ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Bryan Bandli Senior Research Scientist MVA Scientific Consultants 3300 Breckinridge Blvd., Suite 400 (770) 662-8509 bbandli-at-mvainc.com www.mvainc.com ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- The information in this email is confidential and may be legally privileged. It is intended solely for the addressee. If you are not the intended recipient, please delete the email and notify MVA Scientific Consultants of the transmission error. MVA Scientific Consultants - 3300 Breckinridge Blvd. Suite 400, Duluth, GA 30096 - (770)662-8509 -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
==============================Original Headers============================== 6, 18 -- From bbandli-at-mvainc.com Thu Dec 6 13:44:29 2007 6, 18 -- Received: from smtp01.atlngahp.sys.nuvox.net (smtp-out1.atlngahp.sys.nuvox.net [70.43.63.18]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB6JiSGW021953 6, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Dec 2007 13:44:28 -0600 6, 18 -- Received: from [192.168.1.95] (216.215.228.34.nw.nuvox.net [216.215.228.34]) 6, 18 -- by smtp01.atlngahp.sys.nuvox.net (8.13.1/8.13.1) with ESMTP id lB6JiPrM023103 6, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Dec 2007 14:44:25 -0500 6, 18 -- Message-ID: {475850FD.1020302-at-mvainc.com} 6, 18 -- Date: Thu, 06 Dec 2007 14:43:57 -0500 6, 18 -- From: bbandli {bbandli-at-mvainc.com} 6, 18 -- Reply-To: bbandli-at-mvainc.com 6, 18 -- Organization: MVA Scientific Consultants 6, 18 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 6, 18 -- MIME-Version: 1.0 6, 18 -- To: Microscopy-at-microscopy.com 6, 18 -- Subject: Gladstone-Dale calculations 6, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I would use light microscopy to do this, but the particle size is very small and the material is slightly soluble in the refractive index liquids I have access to.
RESPONSE:
You could still use the light microscope with the following changes:
1. Use either
a) a Phase Contrast Microscope, 100x obj
(see Luster, Phase Contrast Microscope Technique for Refractive Index Determination of Anisotropic Particles at Higher Magnification, Microscope and Crystal Front, 13, 2, 363-373, 1963.)
Sub resol particles (average RI, possible to modify to get alpha and gamma or alpha and omega) "Becke line" actually light or dark contrast - very simple, very easy to tell
b) Dispersion staining with a 40X obj as opposed o the standard 10x (plot a chart using diff liquids and wavelengths).
Ted Clarke Published a short piece in Microscopy Today on Darkfield dispersion.
Benedetti-Pilcher, ID of Materials has some other options on RI liquids. Someone at MVA should have one, or tell Jim or Tim to get a copy.
Or
Look at Bloss, Crystallography and Crystal Chemistry, Min Soc Am. 443-445. (I Believe Randy Boltin there has this). Gladstone-Dale relationship plus 3 other relationship are listed.
Also Lorentz-Lorenz Formula (See Viney, Transmitted Poloarised Light Micrscopy)
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
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-----Original Message----- X-from: bbandli-at-mvainc.com [mailto:bbandli-at-mvainc.com] Sent: Thursday, December 06, 2007 2:56 PM To: ph2-at-sprynet.com
Hi All,
I need to determine the average refractive index of a powder of an organic compound. I would use light microscopy to do this, but the particle size is very small and the material is slightly soluble in the refractive index liquids I have access to. I'd like to check my observations against a calculated value. So... is there anyone out there who has used the Gladstone-Dale relationship (avg. refractive index = 1 + specific refractive energy x density) to calculate the refractive index of organic crystals? I would like to be able to calculate an average refractive index of a material given it's composition and density (both of which I can get). There is a significant amount of work on minerals using this relationship, but I haven't found many references on organics, particularly on specific refractive energies of organic compounds. I'm sure there is someone out there who knows more about this than myself.
Thanks in advance for your help! Bryan Bandli
-- ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- --------------------------------------------------------- Bryan Bandli Senior Research Scientist MVA Scientific Consultants 3300 Breckinridge Blvd., Suite 400 (770) 662-8509 bbandli-at-mvainc.com www.mvainc.com ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- --------------------------------------------------------- The information in this email is confidential and may be legally privileged.
It is intended solely for the addressee. If you are not the intended recipient, please delete the email and notify MVA Scientific Consultants of the transmission error. MVA Scientific Consultants - 3300 Breckinridge Blvd. Suite 400, Duluth, GA 30096 - (770)662-8509 ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- -----------------------------------------------------------
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This Question was submitted to Ask-A-Microscopist by (bctorres-at-seton.org.) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, December 6, 2007 at 13:03:26 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both bctorres-at-seton.org. as well as to the Microscopy Listserver ---------------------------------------------------------------------------
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Email: smiller-at-umr.edu Name: Scott Miller
Organization: University of Missouri-Rolla
Title-Subject: [Filtered] Job Opening: Senior Research Scientist
Question: The Graduate Center for Materials Research at the University of Missouri-Rolla (UMR), to be known as Missouri University of Science and Technology (Missouri S&T) effective Jan 1, 2008, is seeking applicants for a non-academic, full time Senior Research Scientist to operate, manage, support, and conduct research in the area of analytical electron microscopy of materials, in particular focused ion beam (FIB) and transmission electron microscopy (TEM). The center has recently acquired a Helios 600 Nanolab FIB with a 12 quadrant STEM detector and ìAuto Slice and Viewî and ìAutoTEMî software routines.
The person in this position will work not only with UMR faculty, staff, and students but also local, state, and regional academic and non-academic institutions to generate, analyze, and publish research findings. The successful candidate will be expected to promote collaborations and support efforts to obtain external funding. This position reports to the Director of the center.
Qualifications will include a Masterís Degree (PhD is preferred) in materials (i.e., metallurgy, ceramics, materials science) or the physical sciences (i.e., physics, chemistry) with at least five years academic or industrial experience in electron microscopy and a publication record in peer reviewed journals. Preference will be given to candidates with experience operating a dual-beam FIB system.
Compensation will be commensurate with qualifications and experience.
Interested candidates should apply online at hr.umr.edu/applicantinfo/index.html
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Email: wcrgs-at-aol.com Name: R. Obie
Organization: WCRG
Title-Subject: [Filtered] LM detection of NaOH and H2O2 on Wood Cross Section
Question: We are experimenting with placing NaOH onto the surface of wood followed by H2O2. Does anyone know of a LM staining protocol that would allow the detection of NaOH such that we could look at the wood sample in cross section to see how far the NaOH has penetrated, either as NaOH or pH? What about the detection of H2O2 in the same scenario? (We have been considering Ti(IV) for this latter objective.) Any comments/insights would be very much appreciated.
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Email: whittaks-at-si.edu Name: Scott Whittaker
Organization: Smithsonian Institution
Title-Subject: [Filtered] TEM usage in the DC area
Question: A handful of researchers here have asked if I would include a TEM lab in my space planning and include it as a service to the units. I am on the fence as to whether there will be enough use to justify, but have a year before a decision needs to be made. Are there any biologic labs in the DC area interested in providing sectioning and scope time on a fee basis? I/we will take care of the embedding/prep, but I have neither a functioning microtome nor the instrument itself.
Thanks,
Scott Whittaker Head NMNH Imaging Manager SEM Lab Smithsonian Institution National Museum of Natural History PO Box 37012 MRC104 Washington DC 20013-7012 202-633-0891
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Email: m_jarnik-at-fccc.edu Name: Michal Jarnik
Organization: Fox Chase Cancer Center
Title-Subject: [Filtered] Philips TEM420 donation
Question: We have an old TEM 420 we would like to donate. The instrument was under service contract until about 2 years ago and should be functional or need just a bit of care. The system is equipped with EDAX and STEM units. Located in North Philadelphia, PA. The accepting party is responsible for dismounting and shipping charges. We need to remove this instrument soon as we need the space.
If interested or have questions, please contact me off list.
} I am looking for a Philps 201, working or not. reply to gary-at-perfendo.com thanks, gbc Gary B. Carr Pacific Endodontic Research Foundation
} Organization: Fox Chase Cancer Center } } Title-Subject: [Filtered] Philips TEM420 donation } } Question: We have an old TEM 420 we would like to donate. The } instrument was under service contract until about 2 years ago and } should be functional or need just a bit of care. The system is } equipped with EDAX and STEM units. Located in North Philadelphia, PA. } The accepting party is responsible for dismounting and shipping } charges. We need to remove this instrument soon as we need the space. } } If interested or have questions, please contact me off list. } } Michal Jarnik } } Login Host: 131.249.80.207 } --------------------------------------------------------------------------- } } } ==============================Original } Headers============================== } 9, 21 -- From zaluzec-at-microscopy.com Fri Dec 7 19:57:55 2007 } 9, 21 -- Received: from s-utl02-sjpop.stsn.net (s-utl02-sjpop.stsn.net } [72.254.0.202]) } 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } lB81vtkG020562 } 9, 21 -- for {microscopy-at-microscopy.com} ; Fri, 7 Dec 2007 19:57:55 -0600 } 9, 21 -- Received: from s-utl02-sjpop.stsn.net ([127.0.0.1]) } 9, 21 -- by s-utl02-sjpop.stsn.net (SMSSMTP 4.1.2.20) with SMTP id } M2007120717575411459 } 9, 21 -- for {microscopy-at-microscopy.com} ; Fri, 07 Dec 2007 17:57:54 -0800 } 9, 21 -- X-Spam-Status: No, hits=0.0 required=9.9 } 9, 21 -- tests=ALL_TRUSTED: -2.867,BAYES_00: -1.665 } 9, 21 -- X-Spam-Level: } 9, 21 -- Received: from [10.5.114.249] ([10.3.158.59]) } 9, 21 -- by s-utl02-sjpop.stsn.net } 9, 21 -- for microscopy-at-microscopy.com; } 9, 21 -- Fri, 7 Dec 2007 17:57:53 -0800 } 9, 21 -- Mime-Version: 1.0 } 9, 21 -- Message-Id: {p0624080ec37faa793571-at-[10.5.114.249]} } 9, 21 -- Date: Fri, 7 Dec 2007 19:57:40 -0600 } 9, 21 -- To: microscopy-at-microscopy.com } 9, 21 -- From: m_jarnik-at-fccc.edu (by way of MicroscopyListserver) } 9, 21 -- Subject: viaWWW: Philips TEM420 donation } 9, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - } Headers==============================
==============================Original Headers============================== 3, 28 -- From gary-at-perfendo.com Fri Dec 7 20:18:21 2007 3, 28 -- Received: from mx.cbeyond.com (mx.cbeyond.com [66.180.96.58]) 3, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB82IL7q000536 3, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Dec 2007 20:18:21 -0600 3, 28 -- Received: from [74.7.224.162] (port=22057 helo=perfendo.com) 3, 28 -- by mx.cbeyond.com with esmtp (Exim 4.62) 3, 28 -- (envelope-from {gary-at-perfendo.com} ) 3, 28 -- id 1J0pGm-0008Fn-UV 3, 28 -- for Microscopy-at-microscopy.com; Fri, 07 Dec 2007 21:18:21 -0500 3, 28 -- Received: from C101DRCARR ([192.168.100.100]) by perfendo.com with Microsoft SMTPSVC(6.0.3790.3959); 3, 28 -- Fri, 7 Dec 2007 18:23:53 -0800 3, 28 -- Message-ID: {001301c83941$5f8eca30$6464a8c0-at-tdooffice.com} 3, 28 -- From: "Dr. Gary Carr" {gary-at-perfendo.com} 3, 28 -- To: {Microscopy-at-microscopy.com} 3, 28 -- References: {200712080207.lB827DuE029757-at-ns.microscopy.com} 3, 28 -- Subject: Re: [Microscopy] viaWWW: Philips TEM420 donation 3, 28 -- Date: Fri, 7 Dec 2007 18:23:51 -0800 3, 28 -- MIME-Version: 1.0 3, 28 -- Content-Type: text/plain; 3, 28 -- format=flowed; 3, 28 -- charset="iso-8859-1"; 3, 28 -- reply-type=original 3, 28 -- Content-Transfer-Encoding: 7bit 3, 28 -- X-Priority: 3 3, 28 -- X-MSMail-Priority: Normal 3, 28 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 3, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 3, 28 -- X-OriginalArrivalTime: 08 Dec 2007 02:23:53.0656 (UTC) FILETIME=[609C1B80:01C83941] ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both qxing-at-ameslab.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: qxing-at-ameslab.gov Name: Qingfeng Xing
Organization: Ames Lab
Title-Subject: [Filtered] Safety of 20% perchoric in methanol
Question: Does anyone know whether it is safe to store the mixture of 20% perchloric acid and 80% methanol at room temperature?
This Question was submitted to Ask-A-Microscopist by (bctorres-at-seton.org.) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, December 6, 2007 at 13:03:26 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both bctorres-at-seton.org. as well as to the Microscopy Listserver ---------------------------------------------------------------------------
} Email: bctorres-at-seton.org. } Name: belinda torres } } Organization: dell children's hospital } } Education: Undergraduate College } } Location: City, State, Country } } Title: cilliary bx } } Question: I need a procedure that yeilds a good representation of the deing arms. can you help me } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-ultra5.microscopy.com Sat Dec 8 18:24:34 2007 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB90OVPM018758 } 8, 11 -- for {microscopy-at-microscopy.com} ; Sat, 8 Dec 2007 18:24:33 -0600 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240801c380e62ab447-at-[206.69.208.22]} } 8, 11 -- Date: Sat, 8 Dec 2007 18:24:31 -0600 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: bctorres-at-seton.org (by way of Ask-A-Microscopist) } 8, 11 -- Subject: AskAMicroscopist: procedure for deing arms } 8, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 5, 21 -- From tina-at-pbrc.hawaii.edu Sat Dec 8 19:20:44 2007 5, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB91Kibx031781 5, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sat, 8 Dec 2007 19:20:44 -0600 5, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id lB91Kedt004269 5, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 21 -- Sat, 8 Dec 2007 15:20:41 -1000 (HST) 5, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id lB91KbFU004266; 5, 21 -- Sat, 8 Dec 2007 15:20:39 -1000 (HST) 5, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 5, 21 -- Date: Sat, 8 Dec 2007 15:20:36 -1000 (HST) 5, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 21 -- X-Sender: tina-at-halia 5, 21 -- To: bctorres-at-seton.org 5, 21 -- cc: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 5, 21 -- Subject: Re: [Microscopy] AskAMicroscopist: procedure for deing arms 5, 21 -- In-Reply-To: {200712090026.lB90QAML020418-at-ns.microscopy.com} 5, 21 -- Message-ID: {Pine.GSO.4.21.0712081518150.4257-100000-at-halia} 5, 21 -- MIME-Version: 1.0 5, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
Dear friends immediately after the FOM 2008 in Japan, please fly to Erice, Sicily for a GREAT School (download poster at www.lambs.it). ---- FIRST ANNOUNCEMENT - School will be restricted to 70 students ------- ----- INFO: diaspro-at-fisica.unige.it --- SUBJECT: ERICE 2008 - CONFOCAL ------
INTERNATIONAL SCHOOL OF BIOPHYSICS «ANTONIO BORSELLINO» 36th Course: MULTIDIMENSIONAL OPTICAL FLUORESCENCE MICROSCOPY TOWARDS NANOSCOPY ERICE–SICILY: 19 – 29 APRIL 2008 Sponsored by the: • Italian Ministry of Education, University and Scientific Research • Sicilian Regional Government, Companies.
LECTURERS Fluorescence Spectroscopy, GFP Photophysics • R. BIZZARRI, NEST-INFM, SNS, Pisa, IT; Optics, Confocal Microscopy, THG • F. BRAKENHOFF, University of Amsterdam, NL; FRAP, Single particle tracking • K. BRAECKMANS, Ghent University, BE; Single molecule force spectroscopy • J. BRUJIC, New York University, USA; Fluctuation Microscopies for biological tissues GIUSEPPE CHIRICO, University of Milan-Bicocca, IT; Micro-particle manipulation • D. COJOC, TASC, INFM, Trieste, IT; SHG, CARS, 2PE • C. COMBS, NIH, Bethesda, USA; 2PE, 3D imaging • A. DIASPRO, University of Genoa, IT; Micro/Nano Optical Manipulation • E. Di FABRIZIO, Unversity of Catanzaro Magna Graecia, IT; Raster Image Correlation Spectroscopy, Photon Counting • M. DIGMAN, UC Irvine, USA; High-content screening • M. FARETTA, IFOM- IEO, Milan, IT; Correlative Microscopy • U. FASCIO, University of Milan, IT; Fluorescence Lifetime, FRET • H.C. GERRITSEN, Utrecht University, NL; FCS, Global Data Analysis • E. GRATTON, UC Irvine, USA; Photonic crystals, nanophotonics • M. GU, Swinburne Univ. of Technology, Victoria, AU; Time lapse imaging • S. GUIDO, Universit of Naples, IT; Fluorescence Optical Nanoscopy • S. HELL, MPI, Goettingen, DE; Scanning Microscopy, Optical aberrations • M. MARTINEZ CORRAL, Univ. of Valencia, ES; Optical systems, Scanning Microscopy • F. QUERCIOLI, CNR-ISC, Florence, IT; 2PE, Fast scanning methods • P. SAGGAU, Baylor College of Med. Houston, Texas, USA; Correlative Microscopy at cryo-Temperatures • A. SARTORI, Institut Pasteur, Paris, FR; Light Scattering, FCS applications • P.L. SAN BIAGIO, CNR-IBF, Palermo, IT; Molecular landscapes by means of AFM • G. SCOLES, Princeton University, USA; Linear and Non linear Optical Microscopy • C. SHEPPARD, Ntl Univ. of Singapore, Singapore; Optical Microscopy, 3D imaging, Photonic Forces • E. STELZER, EMBL, Heidelberg, DE; Laser scissors and tweezers in cell biology • I. TOLIC-NORRELYKKE, MPI, Dresden, DE; Fluorescence imaging in Neuroscience • V. TORRE, SISSA, Trieste, IT; Quantitative colocalization • C. USAI, CNR-IBF, Genoa, IT; Confocal Microscopy, Structured light methods • T. WILSON, University of Oxford, UK; Photoswitch-activatable fluorescent proteins, Lifetime • F. WOUTERS, Univ. of Goettingen, DE. --------
--- ---------------------------------------------------- "Follow knowledge wherever it leads us." (reading Galileo Galilei) ----------------------------------------------------- Alberto Diaspro, EBSA President-Elect, MicroScoBIO LAMBS-IFOM, Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy - fax +39-010314218 - tel +39 0103536426/309; URLs: www.lambs.it ; EBSA is Biophysics in Europe - European Biophysical Societies' Association www.ebsa.org ----------------------------------------------
==============================Original Headers============================== 10, 22 -- From sekkio-at-mac.com Sun Dec 9 14:39:41 2007 10, 22 -- Received: from smtpoutm.mac.com (smtpoutm.mac.com [17.148.16.83]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lB9KdeU8023006 10, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sun, 9 Dec 2007 14:39:40 -0600 10, 22 -- Received: from mac.com (asmtp002-s [10.150.69.65]) 10, 22 -- by smtpoutm.mac.com (Xserve/smtpout020/MantshX 4.0) with ESMTP id lB9Kde5A008391 10, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sun, 9 Dec 2007 12:39:40 -0800 (PST) 10, 22 -- Received: from [10.0.1.2] (host154-123-dynamic.0-87-r.retail.telecomitalia.it [87.0.123.154]) 10, 22 -- (authenticated bits=0) 10, 22 -- by mac.com (Xserve/asmtp002/MantshX 4.0) with ESMTP id lB9KcvGi025690 10, 22 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO) 10, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sun, 9 Dec 2007 12:39:36 -0800 (PST) 10, 22 -- Message-Id: {6E9846C7-8898-4C59-A155-D091504666D1-at-mac.com} 10, 22 -- From: Diaspro {sekkio-at-mac.com} 10, 22 -- To: Microscopy-at-MSA.Microscopy.Com 10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 10, 22 -- Mime-Version: 1.0 (Apple Message framework v915) 10, 22 -- Subject: alby ERICE 2008 10, 22 -- Date: Sun, 9 Dec 2007 21:39:35 +0100 10, 22 -- X-Mailer: Apple Mail (2.915) 10, 22 -- Content-Transfer-Encoding: 8bit 10, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lB9KdeU8023006 ==============================End of - Headers==============================
Qingfeng, It seems questionable. Try checking with GFS Chemicals, www.gfschemicals.com . They manufacture perchlorates and have a lot of safety info available.
Rob Bowen
} From: {qxing-at-ameslab.gov} } Reply-To: {qxing-at-ameslab.gov} } Date: Sat, 8 Dec 2007 09:27:55 -0600 } To: {rob.bowen-at-caddock.com} } Subject: [Microscopy] viaWWW: Safety of 20% perchoric in methanol } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both qxing-at-ameslab.gov as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: qxing-at-ameslab.gov } Name: Qingfeng Xing } } Organization: Ames Lab } } Title-Subject: [Filtered] Safety of 20% perchoric in methanol } } Question: Does anyone know whether it is safe to store the mixture of } 20% perchloric acid and 80% methanol at room temperature? } } Thanks } Qingfeng } } Login Host: 12.226.103.108 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 8, 21 -- From zaluzec-at-microscopy.com Sat Dec 8 09:24:04 2007 } 8, 21 -- Received: from s-utl01-sjpop.stsn.net (s-utl01-sjpop.stsn.net } [72.254.0.201]) } 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } lB8FO4W2028166 } 8, 21 -- for {microscopy-at-microscopy.com} ; Sat, 8 Dec 2007 09:24:04 -0600 } 8, 21 -- Received: from s-utl01-sjpop.stsn.net ([127.0.0.1]) } 8, 21 -- by s-utl01-sjpop.stsn.net (SMSSMTP 4.1.2.20) with SMTP id } M2007120807240323767 } 8, 21 -- for {microscopy-at-microscopy.com} ; Sat, 08 Dec 2007 07:24:03 -0800 } 8, 21 -- X-Spam-Status: No, hits=0.0 required=9.9 } 8, 21 -- tests=ALL_TRUSTED: -2.867,BAYES_00: -1.665 } 8, 21 -- X-Spam-Level: } 8, 21 -- Received: from [10.5.114.249] ([10.3.158.70]) } 8, 21 -- by s-utl01-sjpop.stsn.net } 8, 21 -- for microscopy-at-microscopy.com; } 8, 21 -- Sat, 8 Dec 2007 07:24:02 -0800 } 8, 21 -- Mime-Version: 1.0 } 8, 21 -- Message-Id: {p06240811c3806779e1fd-at-[10.5.114.249]} } 8, 21 -- Date: Sat, 8 Dec 2007 09:23:50 -0600 } 8, 21 -- To: microscopy-at-microscopy.com } 8, 21 -- From: qxing-at-ameslab.gov (by way of MicroscopyListserver) } 8, 21 -- Subject: viaWWW: Safety of 20% perchoric in methanol } 8, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 23 -- From Rob.Bowen-at-caddock.com Mon Dec 10 09:39:48 2007 6, 23 -- Received: from msg.caddock.com (69-29-3-221.stat.centurytel.net [69.29.3.221]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBAFdjOo004940 6, 23 -- for {microscopy-at-microscopy.com} ; Mon, 10 Dec 2007 09:39:47 -0600 6, 23 -- DKIM-Signature: a=rsa-sha1; t=1197301201; x=1197906001; s=m512; d=caddock.com; c=relaxed/relaxed; q=dns; h=From:Subject:Date:Message-ID:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding:In-Reply-To; 6, 23 -- b=Q0/er5rHC5BQd3NtfvYdB7mRcrGfDlUcJ3p3n/wJNQWKUptvD7KANu372PDL+Y0s68/jxcpRTSoGJvqh5sZV7w== 6, 23 -- Received: from [10.1.2.107] ([10.1.2.107]) 6, 23 -- by msg.caddock.com (IceWarp 9.1.0) with ASMTP id PBY28701; 6, 23 -- Mon, 10 Dec 2007 07:40:01 -0800 6, 23 -- User-Agent: Microsoft-Entourage/11.3.3.061214 6, 23 -- Date: Mon, 10 Dec 2007 07:39:42 -0800 6, 23 -- Subject: Re: [Microscopy] viaWWW: Safety of 20% perchoric in methanol 6, 23 -- From: Rob Bowen {Rob.Bowen-at-caddock.com} 6, 23 -- To: {qxing-at-ameslab.gov} 6, 23 -- CC: {microscopy-at-microscopy.com} 6, 23 -- Message-ID: {C3829DBE.6231%Rob.Bowen-at-caddock.com} 6, 23 -- Thread-Topic: [Microscopy] viaWWW: Safety of 20% perchoric in methanol 6, 23 -- Thread-Index: Acg7QuGqIBx45qc2Edy4pgARJDSgFA== 6, 23 -- In-Reply-To: {200712081527.lB8FRtgO000604-at-ns.microscopy.com} 6, 23 -- Mime-version: 1.0 6, 23 -- Content-type: text/plain; 6, 23 -- charset="US-ASCII" 6, 23 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
I am having a problem connecting a 3rd party image acquisition system to our 'new' Hitachi S-2700 SEM.
Long story short, is that we got this scope just as it was about to be tipped into the dumpster. A company was 'consolidating' operations (ie. moving offshore) and had to get out of their leased building ASAP. I grabbed the scope and it is now running well.
Trouble is that the scope was supposed to have a feedback plug on the external scan connection, and it wasn't there. Second trouble is that the scope is not supposed to work without the plug, but it does. Third problem is that when I connect our image acquisition system to the external scan outlet, it does not act the way it should and we cannot collect images.
I didn't think the scope had been modified, no x-ray or any other accessories to suggest anything like that. The only hint of something different was a VCR in the same room as the scope, but I don't know if it was part of the system. So the mystery is what is going on. Anyone got a clue about where to start looking? I have contacted Hitachi hoping they might have some old sales or service records. Something is up with the external scan controls, but I don't know what.
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. Visit http://www.aidslifecycle.org for more information about the ride.
==============================Original Headers============================== 8, 20 -- From jmkrupp-at-ucsc.edu Mon Dec 10 13:42:24 2007 8, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBAJgNom025598 8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 10 Dec 2007 13:42:24 -0600 8, 20 -- Received: from [128.114.125.129] (HELO ucsc.edu) 8, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 8, 20 -- with ESMTPS id 27147103 for microscopy-at-microscopy.com; Mon, 10 Dec 2007 11:42:19 -0800 8, 20 -- Received: by email-prod-fe-2.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 8, 20 -- with PIPE id 31526967; Mon, 10 Dec 2007 11:42:19 -0800 8, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 8, 20 -- Received: from [128.114.25.127] (account jmkrupp-at-ucsc.edu HELO [128.114.25.127]) 8, 20 -- by email-prod-fe-2.ucsc.edu (CommuniGate Pro SMTP 5.1.11) 8, 20 -- with ESMTPA id 31526925 for microscopy-at-microscopy.com; Mon, 10 Dec 2007 11:42:14 -0800 8, 20 -- Mime-Version: 1.0 8, 20 -- Message-Id: {p06230902c383448f7610-at-[128.114.25.127]} 8, 20 -- Date: Mon, 10 Dec 2007 11:42:12 -0800 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 8, 20 -- Subject: Hitachi feedback plug 8, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
About multiple fluorescence we got excellent results using a white light laser coupled to the SP5AOBS I think WLL is really a great advance in spectral imaging icluding related applications ciao Alby Il giorno 20/nov/07, alle ore 15:42, nizets2-at-yahoo.com ha scritto:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi! } } I have used the previous Zeiss 510 microscope and liked it very } much. It is user-friendly and powerful. } I don't know the technical details of the Leica but in the Zeiss the } different wavelengthes are separated not only by filters but also in } time, so no read-through is possible. I must say this gives a } welcome level of confidence and comfort. I did a lot of triple } staining with great results. } } No interest blabla.... } } Regards, } Stephane } } } ----- Original Message ---- } X-from: "christopher.hayden-at-novartis.com" {christopher.hayden-at-novartis.com } } } To: nizets2-at-yahoo.com } Sent: Monday, November 19, 2007 7:28:16 PM } Subject: [Microscopy] LM - Confocal Opinions } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Good Day, All: } } We're in the fortunate position of looking around at } acquiring a } confocal microscope sometime in 2008. Before we start hearing the } pitches } from the manufacturers, we'd like to hear from the "real" world. } Specifically, what you have and what you think of it (ease-of-use, } upgrades, level of control, service and support, etc.). } After having a look around, it seems like the two contenders } for } us are the Zeiss 510 META NLO and the Leica TCS SP5. Flexibility is } key } for us, as it's going to have a fairly broad set of uses as both a } research tool and as a development tool. } Cost isn't critical at this point; we're basically being told } to } "get what we need". While we have people with confocal experience, } each is } } a little biased that the system they know is the best one. } } Thanks much! } -Chris } } } } ==============================Original } Headers============================== } 5, 29 -- From christopher.hayden-at-novartis.com Mon Nov 19 12:23:16 2007 } 5, 29 -- Received: from mail194.messagelabs.com } (mail194.messagelabs.com [85.158.140.211]) } 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id lAJINGAI008522 } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } 12:23:16 -0600 } 5, 29 -- X-VirusChecked: Checked } 5, 29 -- X-Env-Sender: christopher.hayden-at-novartis.com } 5, 29 -- X-Msg-Ref: server-10.tower-194.messagelabs.com!1195496595! } 1972703!1 } 5, 29 -- X-StarScan-Version: 5.5.12.14.2; banners=-,-,- } 5, 29 -- X-Originating-IP: [160.62.1.174] } 5, 29 -- Received: (qmail 15936 invoked from network); 19 Nov 2007 } 18:23:15 -0000 } 5, 29 -- Received: from ch2ssaenov01.novartis.com (HELO } ch2ssaenov01.novartis.com) (160.62.1.174) } 5, 29 -- by server-10.tower-194.messagelabs.com with AES256-SHA } encrypted SMTP; 19 Nov 2007 18:23:15 -0000 } 5, 29 -- Received: from ch1ssainov01.novartis.net (ch1ssainov01s } [192.37.2.173]) } 5, 29 -- by ch2ssaenov01.novartis.com (8.13.8/8.13.8) with ESMTP } id lAJINE00017372 } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } 19:23:14 +0100 } 5, 29 -- Received: from phchbs-s3140.EU.novartis.net (phchbs- } s3140.eu.novartis.net [192.37.31.248]) } 5, 29 -- by ch1ssainov01.novartis.net (8.13.8/8.13.8) with ESMTP } id lAJINE7X016212 } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } 19:23:14 +0100 } 5, 29 -- To: Microscopy-at-Microscopy.Com } 5, 29 -- Subject: LM - Confocal Opinions } 5, 29 -- MIME-Version: 1.0 } 5, 29 -- X-Mailer: Lotus Notes Release 7.0.1 HF517 January 12, 2007 } 5, 29 -- Message-ID: {OFC44BD91C.2AD7BFB4-ON85257398.0064ECC4-85257398.006500CF-at-ah.novartis.com } } } 5, 29 -- From: christopher.hayden-at-novartis.com } 5, 29 -- Date: Mon, 19 Nov 2007 13:23:13 -0500 } 5, 29 -- X-MIMETrack: Serialize by Router on CHBSSPH0/PH/ } Novartis(Release 7.0.2FP2 HF144|July 27, 2007) at } 5, 29 -- 19.11.2007 19:23:14, } 5, 29 -- Serialize complete at 19.11.2007 19:23:14 } 5, 29 -- Content-Type: text/plain; charset="US-ASCII" } ==============================End of - } Headers============================== } } } } ____________________________________________________________________________________ } Be a better pen pal. } Text or chat with friends inside Yahoo! Mail. See how. http://overview.mail.yahoo.com/ } } ==============================Original } Headers============================== } 19, 20 -- From nizets2-at-yahoo.com Tue Nov 20 08:39:39 2007 } 19, 20 -- Received: from web37414.mail.mud.yahoo.com } (web37414.mail.mud.yahoo.com [209.191.91.146]) } 19, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id lAKEdc98014460 } 19, 20 -- for {microscopy-at-microscopy.com} ; Tue, 20 Nov 2007 } 08:39:38 -0600 } 19, 20 -- Received: (qmail 3622 invoked by uid 60001); 20 Nov 2007 } 14:39:38 -0000 } 19, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 19, 20 -- s=s1024; d=yahoo.com; } 19, 20 -- h=X-YMail-OSG:Received:X- } Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; } 19, 20 -- b=QvMKdQxtyTgYygoA+/ } WvTMoT6VrXnErFZYqAO8HBdjZ75Bue5UJBJbgQPJoe1IYxXgEoma8MAFZHlbikrCvN8T1cVFrMKOKxQdtmjmWpBbjdyQw4r7t8pjB } +ZDGttvxwrE1JWZc92VlBK8YAbSzdfqKtnYK4XOUDB2MTaorddgc=; } 19, 20 -- X-YMail-OSG: } _M57H5YVM1kRdmMtoug4Z4BZZz86tUrbpxFAdpWyf5STGplD138_1agXHOztVYU9dxKkhrHX458qgS } .taSMs3DSY4v7 } .MCqALlncXJ2nPgY12t_n2btruL9UjvzbomdQK2sTPqrrvgG } .C_HY_EyOavrLxiafpuKm6R4yH_jGKBupkv4_3lPm7cs- } 19, 20 -- Received: from [80.122.101.100] by } web37414.mail.mud.yahoo.com via HTTP; Tue, 20 Nov 2007 06:39:38 PST } 19, 20 -- X-Mailer: YahooMailRC/818.27 YahooMailWebService/0.7.157 } 19, 20 -- Date: Tue, 20 Nov 2007 06:39:38 -0800 (PST) } 19, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 19, 20 -- Subject: Re: [Microscopy] LM - Confocal Opinions } 19, 20 -- To: christopher.hayden-at-novartis.com } 19, 20 -- Cc: microscopy-at-microscopy.com } 19, 20 -- MIME-Version: 1.0 } 19, 20 -- Content-Type: text/plain; charset=us-ascii } 19, 20 -- Message-ID: {405818.2251.qm-at-web37414.mail.mud.yahoo.com} } ==============================End of - } Headers============================== }
---------------------------------------------------- "Follow knowledge wherever it leads us." (reading Galileo Galilei) ----------------------------------------------------- Alberto Diaspro, EBSA President-Elect, MicroScoBIO LAMBS-IFOM, Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy - fax +39-010314218 - tel +39 0103536426/309; URLs: www.lambs.it ; EBSA is Biophysics in Europe - European Biophysical Societies' Association www.ebsa.org ----------------------------------------------
==============================Original Headers============================== 7, 23 -- From sekkio-at-mac.com Mon Dec 10 14:26:58 2007 7, 23 -- Received: from smtpoutm.mac.com (smtpoutm.mac.com [17.148.16.79]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBAKQvl6006726 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 10 Dec 2007 14:26:57 -0600 7, 23 -- Received: from mac.com (asmtp003-s [10.150.69.66]) 7, 23 -- by smtpoutm.mac.com (Xserve/smtpout016/MantshX 4.0) with ESMTP id lBAKQvYk009658 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 10 Dec 2007 12:26:57 -0800 (PST) 7, 23 -- Received: from [10.0.1.2] (host203-25-dynamic.2-87-r.retail.telecomitalia.it [87.2.25.203]) 7, 23 -- (authenticated bits=0) 7, 23 -- by mac.com (Xserve/asmtp003/MantshX 4.0) with ESMTP id lBAKQop8005304 7, 23 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO) 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 10 Dec 2007 12:26:52 -0800 (PST) 7, 23 -- Message-Id: {BF7EEF6B-25F3-4CCC-BAE5-D907417A5CFA-at-mac.com} 7, 23 -- From: Diaspro {sekkio-at-mac.com} 7, 23 -- To: Microscopy-at-MSA.Microscopy.Com 7, 23 -- In-Reply-To: {200711201442.lAKEgBTc017028-at-ns.microscopy.com} 7, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- Mime-Version: 1.0 (Apple Message framework v915) 7, 23 -- Subject: Re: [Microscopy] Re: LM - Confocal Opinions 7, 23 -- Date: Mon, 10 Dec 2007 21:26:49 +0100 7, 23 -- References: {200711201442.lAKEgBTc017028-at-ns.microscopy.com} 7, 23 -- X-Mailer: Apple Mail (2.915) ==============================End of - Headers==============================
I own a Zeiss 510 Meta NLO and am afraid I don't understand Stephane's comment that the Zeiss separates the signal based on time. Perhaps she means that the system can, in real-time, separate out overlapping emission signals using spectral imaging. If not, perhaps she could expand on her comment.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: sekkio-at-mac.com [mailto:sekkio-at-mac.com] Sent: Monday, December 10, 2007 2:28 PM To: Phillips, Thomas E.
About multiple fluorescence we got excellent results using a white light laser coupled to the SP5AOBS I think WLL is really a great advance in spectral imaging icluding related applications ciao Alby Il giorno 20/nov/07, alle ore 15:42, nizets2-at-yahoo.com ha scritto:
} } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Hi! } } I have used the previous Zeiss 510 microscope and liked it very } much. It is user-friendly and powerful. } I don't know the technical details of the Leica but in the Zeiss the } different wavelengthes are separated not only by filters but also in } time, so no read-through is possible. I must say this gives a } welcome level of confidence and comfort. I did a lot of triple } staining with great results. } } No interest blabla.... } } Regards, } Stephane } } } ----- Original Message ---- } X-from: "christopher.hayden-at-novartis.com" {christopher.hayden-at-novartis.com } } } To: nizets2-at-yahoo.com } Sent: Monday, November 19, 2007 7:28:16 PM } Subject: [Microscopy] LM - Confocal Opinions } } } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Good Day, All: } } We're in the fortunate position of looking around at } acquiring a } confocal microscope sometime in 2008. Before we start hearing the } pitches } from the manufacturers, we'd like to hear from the "real" world. } Specifically, what you have and what you think of it (ease-of-use, } upgrades, level of control, service and support, etc.). } After having a look around, it seems like the two contenders } for } us are the Zeiss 510 META NLO and the Leica TCS SP5. Flexibility is } key } for us, as it's going to have a fairly broad set of uses as both a } research tool and as a development tool. } Cost isn't critical at this point; we're basically being told } to } "get what we need". While we have people with confocal experience, } each is } } a little biased that the system they know is the best one. } } Thanks much! } -Chris } } } } ==============================Original } Headers============================== } 5, 29 -- From christopher.hayden-at-novartis.com Mon Nov 19 12:23:16 2007 } 5, 29 -- Received: from mail194.messagelabs.com } (mail194.messagelabs.com [85.158.140.211]) } 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id lAJINGAI008522 } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } 12:23:16 -0600 } 5, 29 -- X-VirusChecked: Checked } 5, 29 -- X-Env-Sender: christopher.hayden-at-novartis.com } 5, 29 -- X-Msg-Ref: server-10.tower-194.messagelabs.com!1195496595! } 1972703!1 } 5, 29 -- X-StarScan-Version: 5.5.12.14.2; banners=-,-,- } 5, 29 -- X-Originating-IP: [160.62.1.174] } 5, 29 -- Received: (qmail 15936 invoked from network); 19 Nov 2007 } 18:23:15 -0000 } 5, 29 -- Received: from ch2ssaenov01.novartis.com (HELO } ch2ssaenov01.novartis.com) (160.62.1.174) } 5, 29 -- by server-10.tower-194.messagelabs.com with AES256-SHA } encrypted SMTP; 19 Nov 2007 18:23:15 -0000 } 5, 29 -- Received: from ch1ssainov01.novartis.net (ch1ssainov01s } [192.37.2.173]) } 5, 29 -- by ch2ssaenov01.novartis.com (8.13.8/8.13.8) with ESMTP } id lAJINE00017372 } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } 19:23:14 +0100 } 5, 29 -- Received: from phchbs-s3140.EU.novartis.net (phchbs- } s3140.eu.novartis.net [192.37.31.248]) } 5, 29 -- by ch1ssainov01.novartis.net (8.13.8/8.13.8) with ESMTP } id lAJINE7X016212 } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } 19:23:14 +0100 } 5, 29 -- To: Microscopy-at-Microscopy.Com } 5, 29 -- Subject: LM - Confocal Opinions } 5, 29 -- MIME-Version: 1.0 } 5, 29 -- X-Mailer: Lotus Notes Release 7.0.1 HF517 January 12, 2007 } 5, 29 -- Message-ID: {OFC44BD91C.2AD7BFB4-ON85257398.0064ECC4-85257398.006500CF-at-ah.novartis.c om } } } 5, 29 -- From: christopher.hayden-at-novartis.com } 5, 29 -- Date: Mon, 19 Nov 2007 13:23:13 -0500 } 5, 29 -- X-MIMETrack: Serialize by Router on CHBSSPH0/PH/ } Novartis(Release 7.0.2FP2 HF144|July 27, 2007) at } 5, 29 -- 19.11.2007 19:23:14, } 5, 29 -- Serialize complete at 19.11.2007 19:23:14 } 5, 29 -- Content-Type: text/plain; charset="US-ASCII" } ==============================End of - } Headers============================== } } } } ________________________________________________________________________ ____________ } Be a better pen pal. } Text or chat with friends inside Yahoo! Mail. See how. http://overview.mail.yahoo.com/ } } ==============================Original } Headers============================== } 19, 20 -- From nizets2-at-yahoo.com Tue Nov 20 08:39:39 2007 } 19, 20 -- Received: from web37414.mail.mud.yahoo.com } (web37414.mail.mud.yahoo.com [209.191.91.146]) } 19, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
---------------------------------------------------- "Follow knowledge wherever it leads us." (reading Galileo Galilei) ----------------------------------------------------- Alberto Diaspro, EBSA President-Elect, MicroScoBIO LAMBS-IFOM, Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy - fax +39-010314218 - tel +39 0103536426/309; URLs: www.lambs.it ; EBSA is Biophysics in Europe - European Biophysical Societies' Association www.ebsa.org ----------------------------------------------
==============================Original Headers============================== 7, 23 -- From sekkio-at-mac.com Mon Dec 10 14:26:58 2007 7, 23 -- Received: from smtpoutm.mac.com (smtpoutm.mac.com [17.148.16.79]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBAKQvl6006726 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 10 Dec 2007 14:26:57 -0600 7, 23 -- Received: from mac.com (asmtp003-s [10.150.69.66]) 7, 23 -- by smtpoutm.mac.com (Xserve/smtpout016/MantshX 4.0) with ESMTP id lBAKQvYk009658 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 10 Dec 2007 12:26:57 -0800 (PST) 7, 23 -- Received: from [10.0.1.2] (host203-25-dynamic.2-87-r.retail.telecomitalia.it [87.2.25.203]) 7, 23 -- (authenticated bits=0) 7, 23 -- by mac.com (Xserve/asmtp003/MantshX 4.0) with ESMTP id lBAKQop8005304 7, 23 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO) 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 10 Dec 2007 12:26:52 -0800 (PST) 7, 23 -- Message-Id: {BF7EEF6B-25F3-4CCC-BAE5-D907417A5CFA-at-mac.com} 7, 23 -- From: Diaspro {sekkio-at-mac.com} 7, 23 -- To: Microscopy-at-MSA.Microscopy.Com 7, 23 -- In-Reply-To: {200711201442.lAKEgBTc017028-at-ns.microscopy.com} 7, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- Mime-Version: 1.0 (Apple Message framework v915) 7, 23 -- Subject: Re: [Microscopy] Re: LM - Confocal Opinions 7, 23 -- Date: Mon, 10 Dec 2007 21:26:49 +0100 7, 23 -- References: {200711201442.lAKEgBTc017028-at-ns.microscopy.com} 7, 23 -- X-Mailer: Apple Mail (2.915) ==============================End of - Headers==============================
==============================Original Headers============================== 17, 26 -- From PhillipsT-at-missouri.edu Mon Dec 10 14:55:06 2007 17, 26 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBAKt6tE020017 17, 26 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 10 Dec 2007 14:55:06 -0600 17, 26 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 17, 26 -- Mon, 10 Dec 2007 14:55:06 -0600 17, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 26 -- Content-class: urn:content-classes:message 17, 26 -- MIME-Version: 1.0 17, 26 -- Content-Type: text/plain; 17, 26 -- charset="us-ascii" 17, 26 -- Subject: RE: [Microscopy] LM - Confocal Opinions 17, 26 -- Date: Mon, 10 Dec 2007 14:55:05 -0600 17, 26 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD0331F9E4-at-UM-XMAIL06.um.umsystem.edu} 17, 26 -- In-Reply-To: {200712102027.lBAKRiH7008002-at-ns.microscopy.com} 17, 26 -- X-MS-Has-Attach: 17, 26 -- X-MS-TNEF-Correlator: 17, 26 -- Thread-Topic: [Microscopy] LM - Confocal Opinions 17, 26 -- thread-index: Acg7ayAuM5lprirLTpCkeUPeEkINOwAAxEhQ 17, 26 -- References: {200712102027.lBAKRiH7008002-at-ns.microscopy.com} 17, 26 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 17, 26 -- To: {sekkio-at-mac.com} 17, 26 -- Cc: {nizets2-at-yahoo.com} , {Microscopy-at-msa.microscopy.com} 17, 26 -- X-OriginalArrivalTime: 10 Dec 2007 20:55:06.0326 (UTC) FILETIME=[F171AF60:01C83B6E] 17, 26 -- Content-Transfer-Encoding: 8bit 17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lBAKt6tE020017 ==============================End of - Headers==============================
As a user of both a Leica and Zeiss system, in terms of user-friendly software, I'm most happy with the Zeiss LSM510 I'm working with.
In our lab we have a more basic Zeiss LSM510 and a Zeiss LSM510 META NLO. The Leica I'm working with is based in another lab; so though I'm more familiar with the Zeiss-thing, I can speak a little for the Leica-confocal microscope too I believe.
On a regular base, let's say about twice, three times a year, I give up to 10-15 new users a training on the basic confocal system (LSM510) which takes about 45-60 minutes. During the first weeks after this training, I attend their first use and help them for 15 minutes where necessary. Merely, this is just to remind them how to best adjust the detector/amplifier gains and amplifier offset and where the buttons for creating a z-stack went to. On average, new users are acquainted with the system in a very short period.
The new ZEN-software in my opinion, installed on the Zeiss LSM510 META NLO, is not only a step closer to user-friendly software, it's a giant leap. Amazing how fast you find what you need, you hide what you don't need but want to keep available and how you browse through all possible settings in a very organized way. On top of that, all on a background that doesn't blind you (soft gray versus the old & hard white).
Concerning the Leica-software, I do like somehow the block-setup (step by step), though it increases the amount of clicking and browsing around to set up all, adjust & scan, and it shows quite confusing. I prefer not to speak about the quality of the images: this system already has some quite old setup, so it's not really comparable.
I do believe in one thing: take your samples and pass by labs where they have or a Leica or (and/or) a Zeiss, and have a look at your own samples, this'll show you what's the best system for you. If possible, also take someone who doesn't know the systems and have him/her trained during one hour. It's not too long, and this'll show you afterwards which system is most user-friendly.
And also here: no commercial interest, my statements are purely based on experience.
Greetings,
Sven Terclavers
} } } } Good Day, All: } } } } We're in the fortunate position of looking around at } } acquiring a } } confocal microscope sometime in 2008. Before we start hearing the } } pitches } } from the manufacturers, we'd like to hear from the "real" world. } } Specifically, what you have and what you think of it (ease-of-use, } } upgrades, level of control, service and support, etc.). } } After having a look around, it seems like the two contenders } } for } } us are the Zeiss 510 META NLO and the Leica TCS SP5. Flexibility is } } key } } for us, as it's going to have a fairly broad set of uses as both a } } research tool and as a development tool. } } Cost isn't critical at this point; we're basically being told } } to } } "get what we need". While we have people with confocal experience, } } each is } } } } a little biased that the system they know is the best one. } } } } Thanks much! } } -Chris } } } } } } } } ==============================Original } } Headers============================== } } 5, 29 -- From christopher.hayden-at-novartis.com Mon Nov 19 12:23:16 2007 } } 5, 29 -- Received: from mail194.messagelabs.com } } (mail194.messagelabs.com [85.158.140.211]) } } 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id lAJINGAI008522 } } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } } 12:23:16 -0600 } } 5, 29 -- X-VirusChecked: Checked } } 5, 29 -- X-Env-Sender: christopher.hayden-at-novartis.com } } 5, 29 -- X-Msg-Ref: server-10.tower-194.messagelabs.com!1195496595! } } 1972703!1 } } 5, 29 -- X-StarScan-Version: 5.5.12.14.2; banners=-,-,- } } 5, 29 -- X-Originating-IP: [160.62.1.174] } } 5, 29 -- Received: (qmail 15936 invoked from network); 19 Nov 2007 } } 18:23:15 -0000 } } 5, 29 -- Received: from ch2ssaenov01.novartis.com (HELO } } ch2ssaenov01.novartis.com) (160.62.1.174) } } 5, 29 -- by server-10.tower-194.messagelabs.com with AES256-SHA } } encrypted SMTP; 19 Nov 2007 18:23:15 -0000 } } 5, 29 -- Received: from ch1ssainov01.novartis.net (ch1ssainov01s } } [192.37.2.173]) } } 5, 29 -- by ch2ssaenov01.novartis.com (8.13.8/8.13.8) with ESMTP } } id lAJINE00017372 } } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } } 19:23:14 +0100 } } 5, 29 -- Received: from phchbs-s3140.EU.novartis.net (phchbs- } } s3140.eu.novartis.net [192.37.31.248]) } } 5, 29 -- by ch1ssainov01.novartis.net (8.13.8/8.13.8) with ESMTP } } id lAJINE7X016212 } } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } } 19:23:14 +0100 } } 5, 29 -- To: Microscopy-at-Microscopy.Com } } 5, 29 -- Subject: LM - Confocal Opinions } } 5, 29 -- MIME-Version: 1.0 } } 5, 29 -- X-Mailer: Lotus Notes Release 7.0.1 HF517 January 12, 2007 } } 5, 29 -- Message-ID: } {OFC44BD91C.2AD7BFB4-ON85257398.0064ECC4-85257398.006500CF-at-ah.novartis.c } om } } } } } 5, 29 -- From: christopher.hayden-at-novartis.com } } 5, 29 -- Date: Mon, 19 Nov 2007 13:23:13 -0500 } } 5, 29 -- X-MIMETrack: Serialize by Router on CHBSSPH0/PH/ } } Novartis(Release 7.0.2FP2 HF144|July 27, 2007) at } } 5, 29 -- 19.11.2007 19:23:14, } } 5, 29 -- Serialize complete at 19.11.2007 19:23:14 } } 5, 29 -- Content-Type: text/plain; charset="US-ASCII" } } ==============================End of - } } Headers============================== } } } } } } } } } ________________________________________________________________________ } ____________ } } Be a better pen pal. } } Text or chat with friends inside Yahoo! 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See how. } http://overview.mail.yahoo.com/ } } } } ==============================Original } } Headers============================== } } 19, 20 -- From nizets2-at-yahoo.com Tue Nov 20 08:39:39 2007 } } 19, 20 -- Received: from web37414.mail.mud.yahoo.com } } (web37414.mail.mud.yahoo.com [209.191.91.146]) } } 19, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } } } id lAKEdc98014460 } } 19, 20 -- for {microscopy-at-microscopy.com} ; Tue, 20 Nov 2007 } } 08:39:38 -0600 } } 19, 20 -- Received: (qmail 3622 invoked by uid 60001); 20 Nov 2007 } } 14:39:38 -0000 } } 19, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } } 19, 20 -- s=s1024; d=yahoo.com; } } 19, 20 -- h=X-YMail-OSG:Received:X- } } Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; } } 19, 20 -- b=QvMKdQxtyTgYygoA+/ } } } WvTMoT6VrXnErFZYqAO8HBdjZ75Bue5UJBJbgQPJoe1IYxXgEoma8MAFZHlbikrCvN8T1cVF } rMKOKxQdtmjmWpBbjdyQw4r7t8pjB } } +ZDGttvxwrE1JWZc92VlBK8YAbSzdfqKtnYK4XOUDB2MTaorddgc=; } } 19, 20 -- X-YMail-OSG: } } } _M57H5YVM1kRdmMtoug4Z4BZZz86tUrbpxFAdpWyf5STGplD138_1agXHOztVYU9dxKkhrHX } 458qgS } } .taSMs3DSY4v7 } } .MCqALlncXJ2nPgY12t_n2btruL9UjvzbomdQK2sTPqrrvgG } } .C_HY_EyOavrLxiafpuKm6R4yH_jGKBupkv4_3lPm7cs- } } 19, 20 -- Received: from [80.122.101.100] by } } web37414.mail.mud.yahoo.com via HTTP; Tue, 20 Nov 2007 06:39:38 PST } } 19, 20 -- X-Mailer: YahooMailRC/818.27 YahooMailWebService/0.7.157 } } 19, 20 -- Date: Tue, 20 Nov 2007 06:39:38 -0800 (PST) } } 19, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} } } 19, 20 -- Subject: Re: [Microscopy] LM - Confocal Opinions } } 19, 20 -- To: christopher.hayden-at-novartis.com } } 19, 20 -- Cc: microscopy-at-microscopy.com } } 19, 20 -- MIME-Version: 1.0 } } 19, 20 -- Content-Type: text/plain; charset=us-ascii } } 19, 20 -- Message-ID: {405818.2251.qm-at-web37414.mail.mud.yahoo.com} } } ==============================End of - } } Headers============================== } } } } ---------------------------------------------------- } "Follow knowledge wherever it leads us." (reading Galileo Galilei) } ----------------------------------------------------- } Alberto Diaspro, EBSA President-Elect, MicroScoBIO LAMBS-IFOM, } Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 } Genoa, Italy - fax +39-010314218 - tel +39 0103536426/309; URLs: } www.lambs.it } ; } EBSA is Biophysics in Europe - European Biophysical Societies' } Association www.ebsa.org } ---------------------------------------------- } } } } } } ==============================Original } Headers============================== } 7, 23 -- From sekkio-at-mac.com Mon Dec 10 14:26:58 2007 } 7, 23 -- Received: from smtpoutm.mac.com (smtpoutm.mac.com } [17.148.16.79]) } 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id lBAKQvl6006726 } 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 10 Dec 2007 } 14:26:57 -0600 } 7, 23 -- Received: from mac.com (asmtp003-s [10.150.69.66]) } 7, 23 -- by smtpoutm.mac.com (Xserve/smtpout016/MantshX 4.0) with } ESMTP id lBAKQvYk009658 } 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 10 Dec 2007 } 12:26:57 -0800 (PST) } 7, 23 -- Received: from [10.0.1.2] } (host203-25-dynamic.2-87-r.retail.telecomitalia.it [87.2.25.203]) } 7, 23 -- (authenticated bits=0) } 7, 23 -- by mac.com (Xserve/asmtp003/MantshX 4.0) with ESMTP id } lBAKQop8005304 } 7, 23 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 } verify=NO) } 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 10 Dec 2007 } 12:26:52 -0800 (PST) } 7, 23 -- Message-Id: {BF7EEF6B-25F3-4CCC-BAE5-D907417A5CFA-at-mac.com} } 7, 23 -- From: Diaspro {sekkio-at-mac.com} } 7, 23 -- To: Microscopy-at-MSA.Microscopy.Com } 7, 23 -- In-Reply-To: {200711201442.lAKEgBTc017028-at-ns.microscopy.com} } 7, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; } delsp=yes } 7, 23 -- Content-Transfer-Encoding: 7bit } 7, 23 -- Mime-Version: 1.0 (Apple Message framework v915) } 7, 23 -- Subject: Re: [Microscopy] Re: LM - Confocal Opinions } 7, 23 -- Date: Mon, 10 Dec 2007 21:26:49 +0100 } 7, 23 -- References: {200711201442.lAKEgBTc017028-at-ns.microscopy.com} } 7, 23 -- X-Mailer: Apple Mail (2.915) } ==============================End of - } Headers============================== } } } ==============================Original Headers============================== } 17, 26 -- From PhillipsT-at-missouri.edu Mon Dec 10 14:55:06 2007 } 17, 26 -- Received: from um-nsmtpout1.um.umsystem.edu } (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) } 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id lBAKt6tE020017 } 17, 26 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 10 Dec 2007 } 14:55:06 -0600 } 17, 26 -- Received: from UM-XMAIL06.um.umsystem.edu } ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft } SMTPSVC(6.0.3790.3959); } 17, 26 -- Mon, 10 Dec 2007 14:55:06 -0600 } 17, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 17, 26 -- Content-class: urn:content-classes:message } 17, 26 -- MIME-Version: 1.0 } 17, 26 -- Content-Type: text/plain; } 17, 26 -- charset="us-ascii" } 17, 26 -- Subject: RE: [Microscopy] LM - Confocal Opinions } 17, 26 -- Date: Mon, 10 Dec 2007 14:55:05 -0600 } 17, 26 -- Message-ID: } {0510DC719E56F64BB2AD84EE64CE6BAD0331F9E4-at-UM-XMAIL06.um.umsystem.edu} } 17, 26 -- In-Reply-To: {200712102027.lBAKRiH7008002-at-ns.microscopy.com} } 17, 26 -- X-MS-Has-Attach: } 17, 26 -- X-MS-TNEF-Correlator: } 17, 26 -- Thread-Topic: [Microscopy] LM - Confocal Opinions } 17, 26 -- thread-index: Acg7ayAuM5lprirLTpCkeUPeEkINOwAAxEhQ } 17, 26 -- References: {200712102027.lBAKRiH7008002-at-ns.microscopy.com} } 17, 26 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } 17, 26 -- To: {sekkio-at-mac.com} } 17, 26 -- Cc: {nizets2-at-yahoo.com} , {Microscopy-at-msa.microscopy.com} } 17, 26 -- X-OriginalArrivalTime: 10 Dec 2007 20:55:06.0326 (UTC) } FILETIME=[F171AF60:01C83B6E] } 17, 26 -- Content-Transfer-Encoding: 8bit } 17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id lBAKt6tE020017 } ==============================End of - Headers============================== } }
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gerard.cox-at-goodrich.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
We recently got an upgrade to our Image Pro Plus software to the 6.2 level. I asked the sales rep about determining grain size per ASTM standards from metallographic mounts, and he said it could be done with one or more macros. I know that there are commercial options for this, but if I could do it without more cost that would be better. Over my few months on this server I have noticed that most of the replies seem to be more focussed on the biological end of things, but hopefully there are a few metallurgists out there who can help me out. I think that the line intercept procedure would be easier to implement than the circle intercept, but I don't know how to program it. Any guidance or assistance would be greatly appreciated.
Dear Colleagues I have a small home based microscopy laboratory in Athens, Greece and own an SEM microscope Jeol JSM5600 Low Vacuum bought second hand from USA.
Unfortunately the installation CD, marked as version 2.05, has been damaged and I cannot reinstall the software.
If anyone has the same JEOL model with CD install disk version 2.05, please contact me directly. Best regards yorgos
Dr Yorgos Nikas Athens Innovative Microscopy Skra 36 Voula 16673 GREECE eikonika-at-otenet.gr Tel/fax +30 210 8957677 Mobile +30 6945 107477
==============================Original Headers============================== 5, 22 -- From eikonika-at-otenet.gr Tue Dec 11 08:14:50 2007 5, 22 -- Received: from aiolos.otenet.gr (aiolos.otenet.gr [195.170.0.93]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBBEEnM3019024 5, 22 -- for {microscopy-at-microscopy.com} ; Tue, 11 Dec 2007 08:14:50 -0600 5, 22 -- Received: from axeron (athedsl-338994.home.otenet.gr [85.72.179.208]) 5, 22 -- by aiolos.otenet.gr (8.13.8/8.13.8/Debian-3) with SMTP id lBBEEm2Z019392 5, 22 -- for {microscopy-at-microscopy.com} ; Tue, 11 Dec 2007 16:14:48 +0200 5, 22 -- Message-ID: {004a01c83c00$365bedc0$1601a8c0-at-axeron} 5, 22 -- From: "yorgos nikas" {eikonika-at-otenet.gr} 5, 22 -- To: {microscopy-at-microscopy.com} 5, 22 -- Subject: Jeol JSM5600 Low Vacuum installation software urgently needed 5, 22 -- Date: Tue, 11 Dec 2007 16:14:58 +0200 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: text/plain; 5, 22 -- format=flowed; 5, 22 -- charset="iso-8859-7"; 5, 22 -- reply-type=original 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-Priority: 3 5, 22 -- X-MSMail-Priority: Normal 5, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3028 5, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 ==============================End of - Headers==============================
Dear All, I am going to try to use TEM with negative staining to quantify a preparation of influenza virus using polystyrene latex particles (112um) and was wondering if there were any special considerations to take into account? I know that I need to sonicate the particles before use to prevent clumps forming, but was not sure whether it was simply a case of mixing them with the virus at an approximate particle:virus particle ratio of 1:1 and then adding to a carbon coated grid followed by negative staining? Also, how many fields of view or latex particles would be a good number to count to give me some confidence in the numbers seen, representing the whole sample? Email address is below. Thanks for any help. Paul
Dr Paul Scott Research Fellow Pneumovirus Laboratory Department of Biological Sciences The University of Warwick Gibbet Hill Road Coventry CV4 7AL Tel: 02476 522696 Mob: 07930195005 Email: P.D.Scott-at-warwick.ac.uk
==============================Original Headers============================== 5, 36 -- From P.D.Scott-at-warwick.ac.uk Tue Dec 11 08:38:08 2007 5, 36 -- Received: from mail-relay-1.csv.warwick.ac.uk (mail-relay-1.csv.warwick.ac.uk [137.205.128.7]) 5, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBBEc7Qi031864 5, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Dec 2007 08:38:08 -0600 5, 36 -- Received: from localhost (localhost [127.0.0.1]) 5, 36 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id lBBEbnEg090273 5, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Dec 2007 14:38:06 GMT 5, 36 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 5, 36 -- Received: from mail-relay-1.csv.warwick.ac.uk ([127.0.0.1]) 5, 36 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 5, 36 -- with LMTP id gcjhpeO9WRqs for {Microscopy-at-microscopy.com} ; 5, 36 -- Tue, 11 Dec 2007 14:37:48 +0000 (GMT) 5, 36 -- Received: from beech.ads.warwick.ac.uk (beech [137.205.97.163]) 5, 36 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id lBBEbKGW090007 5, 36 -- for {Microscopy-at-Microscopy.Com} ; Tue, 11 Dec 2007 14:37:20 GMT 5, 36 -- X-Envelope-From: P.D.Scott-at-warwick.ac.uk 5, 36 -- Received: from ELDER.ads.warwick.ac.uk ([137.205.97.183]) by beech.ads.warwick.ac.uk with Microsoft SMTPSVC(6.0.3790.1830); 5, 36 -- Tue, 11 Dec 2007 14:37:19 +0000 5, 36 -- Content-class: urn:content-classes:message 5, 36 -- MIME-Version: 1.0 5, 36 -- Content-Type: text/plain; 5, 36 -- charset="us-ascii" 5, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 36 -- Subject: TEM - using latex particlesto quantify influenza virus 5, 36 -- Date: Tue, 11 Dec 2007 14:37:19 -0000 5, 36 -- Message-ID: {2F1B9CD5420AB94CBFA870EDC82E3F9248FE97-at-ELDER.ads.warwick.ac.uk} 5, 36 -- X-MS-Has-Attach: 5, 36 -- X-MS-TNEF-Correlator: 5, 36 -- Thread-Topic: TEM - using latex particlesto quantify influenza virus 5, 36 -- Thread-Index: Acg8A1ThCFkzAmFhSgmeTipyiN8k+A== 5, 36 -- From: "Scott, Paul" {P.D.Scott-at-warwick.ac.uk} 5, 36 -- To: {Microscopy-at-microscopy.com} 5, 36 -- X-OriginalArrivalTime: 11 Dec 2007 14:37:19.0279 (UTC) FILETIME=[553E8BF0:01C83C03] 5, 36 -- X-DCC-Warwick-Metrics: l; whitelist 5, 36 -- Content-Transfer-Encoding: 8bit 5, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lBBEc7Qi031864 ==============================End of - Headers==============================
I would like to start a mailing list and website for those of you who are affiliated with high school programs that have electron microscopes. I know there are some of you out there, as I have been emailing a few of you. I want to have a forum where we can exchange methods for instruction, lessons, equipment sources, and other topics that are of specific interest to the high school and middle school communities.
If you would be interested in participating in such a list, please email me off of this list. I will assemble the names, then email out to you when it's up and running so you can sign up.
Thanks,
Justin A. Kraft Professional Lab Rat Physics Teacher Leadership Academy West West Palm Beach, FL
==============================Original Headers============================== 4, 27 -- From kraftpiano-at-gmail.com Tue Dec 11 08:57:03 2007 4, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.187]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBBEv39x012054 4, 27 -- for {microscopy-at-microscopy.com} ; Tue, 11 Dec 2007 08:57:03 -0600 4, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so2529874rvb 4, 27 -- for {microscopy-at-microscopy.com} ; Tue, 11 Dec 2007 06:57:03 -0800 (PST) 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- bh=79dX1uRfqSVUHX0X6G9BLeNrwArHUw/VtxoAFnq4v3k=; 4, 27 -- b=Lm4ZqfywcMb2AxWhj7IVvkUzs594hahIQvN5bDa00biwLaRbrCMlBMBGAHc0svlYHWuVHjrrZD/sNPZuynkR67Ka5S/rEEl082UajNpzn4oTsVHbXo86nRkbYP8UQ0aGcCwKvFKEpojyyc9hMwehqDT+BSp3eXxBW4lJjfyQWzg= 4, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- b=XkYU7aqkKFyKRRVYtr3T1Xl1ZlMJULySre77/FfgjAcYsfTLjHGQ2rS4W/cdh5ez63h8UznrGAranKqGnvOfLn7LSZ/0Fs5f54O+vBHle4o0DBpWWAcjmEY9ZmCK4mr9vcw94rQAe/elTFsX20dxxiuTs1Qs/tXiB+okhB6znHo= 4, 27 -- Received: by 10.141.122.20 with SMTP id z20mr2360861rvm.1197385023100; 4, 27 -- Tue, 11 Dec 2007 06:57:03 -0800 (PST) 4, 27 -- Received: by 10.140.161.11 with HTTP; Tue, 11 Dec 2007 06:57:03 -0800 (PST) 4, 27 -- Message-ID: {25e2b0d20712110657m57f96b9lfb8fe36538b7988d-at-mail.gmail.com} 4, 27 -- Date: Tue, 11 Dec 2007 09:57:03 -0500 4, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 4, 27 -- To: microscopy-at-microscopy.com 4, 27 -- Subject: A call out to all high schools with electron microscopes (Or advanced microscopy programs) 4, 27 -- MIME-Version: 1.0 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1 4, 27 -- Content-Transfer-Encoding: 7bit 4, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Fifty years ago I did a lot of work polishing the alloys used then in the manufacture of jet engines. These alloys were so corrosion-resistant that about the only thing strong enough to electrolytically polish them were the various solutions based on perchloric acid. Therefore, I worked with these solutions a LOT.
Basically, perchloric acid becomes an explosive when it becomes heated when it is concentrated and in contact with an easily oxidizable material (such as cotton, paper, most organic solvents, most kinds of cloth, etc.). Therefore it is imperative to avoid these conditions! If the hot, concentrated stuff comes in contact with such materials it can produce a very violent explosion. In one instance a graduate student filtered a small amount of a perchlorate salt out of a solution, then placed the damp filter paper that carried the salt in a drying oven. Luckily, he left the room before the stuff let loose, whereupon it drove the door of the oven across the room and embedded it in the opposite cinder block wall. (However, we routinely used boiling perchloric acid to digest mineral samples in certain analytical procedures - no organic or oxidizable material present)
In the work I did, we always kept the polishing solution cooled to below 10°C during the electrolysis process. For solutions made with acetic anhydride we kept the temperature at the point where crystals of the anhydride just started to form in it. We stirred the solutions vigorously during the electrolysis process to prevent localized heat build-up at the electrode surfaces. And we worked inside a large stainless steel or polyethylene pan with a half-inch of water in the bottom, which we washed thoroughly after an experiment was completed so that no residue of the solution remained around to become concentrated by evaporation.
Before a perchloric acid solution actually explodes it will start to develop a brownish-red color. Therefore we always kept a large beaker full of ice water sitting by our polishing apparatus, which we would have poured into the polishing solution to dilute and cool it if this ever happened, and we kept the door of the lab open so that we could then leave in a hurry
We stored our stock solutions in glass bottles with glass stoppers. It is important to avoid bottles with ordinary caps made of organic materials. We always rinsed the bottles thoroughly with water, to remove any traces of solution that might have dribbled down the sides of them during solution transfer. With these precautions we stored perchloric acid solutions of all kinds for many months with no problems.
Perchloric acid is a very useful reagent if you use it with proper precautions and due respect. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 7, 16 -- From bigelow-at-umich.edu Tue Dec 11 15:39:58 2007 7, 16 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82]) 7, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBBLdwev004203 7, 16 -- for {microscopy-at-microscopy.com} ; Tue, 11 Dec 2007 15:39:58 -0600 7, 16 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 7, 16 -- BY hellskitchen.mr.itd.umich.edu ID 475F03AD.29105.8837 ; 7, 16 -- 11 Dec 2007 16:39:57 -0500 7, 16 -- Mime-Version: 1.0 7, 16 -- Message-Id: {p06240804c384a7d0eafa-at-[141.212.131.221]} 7, 16 -- Date: Tue, 11 Dec 2007 16:39:55 -0500 7, 16 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 7, 16 -- From: Wil Bigelow {bigelow-at-umich.edu} 7, 16 -- Subject: [Microscopy] RE: Perchloric solutions 7, 16 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 7, 16 -- Content-Transfer-Encoding: 8bit 7, 16 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lBBLdwev004203 ==============================End of - Headers==============================
Great summary, Wil. If you don't mind, I would like to send this to a customer that I have been talking to about electropolishing and one of the electrolytes that are recommended is perchloric solutions.
I would like to add another point. One of the safety things that you missed is that you should work in a perchloric acid rated hood. These hoods are designed so that they can be periodically washed down in the exhaust area.
In the Number 5 book in the Philips TEM series, which is about TEM sample preparation, they also have a small section on the safety precautions for perchloric acid solutions as well as others.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: bigelow-at-umich.edu [mailto:bigelow-at-umich.edu] Sent: Tuesday, December 11, 2007 1:45 PM To: Walck-at-SouthBayTech.com
Fifty years ago I did a lot of work polishing the alloys used then in the manufacture of jet engines. These alloys were so corrosion-resistant that about the only thing strong enough to electrolytically polish them were the various solutions based on perchloric acid. Therefore, I worked with these solutions a LOT.
Basically, perchloric acid becomes an explosive when it becomes heated when it is concentrated and in contact with an easily oxidizable material (such as cotton, paper, most organic solvents, most kinds of cloth, etc.). Therefore it is imperative to avoid these conditions! If the hot, concentrated stuff comes in contact with such materials it can produce a very violent explosion. In one instance a graduate student filtered a small amount of a perchlorate salt out of a solution, then placed the damp filter paper that carried the salt in a drying oven. Luckily, he left the room before the stuff let loose, whereupon it drove the door of the oven across the room and embedded it in the opposite cinder block wall. (However, we routinely used boiling perchloric acid to digest mineral samples in certain analytical procedures - no organic or oxidizable material present)
In the work I did, we always kept the polishing solution cooled to below 10°C during the electrolysis process. For solutions made with acetic anhydride we kept the temperature at the point where crystals of the anhydride just started to form in it. We stirred the solutions vigorously during the electrolysis process to prevent localized heat build-up at the electrode surfaces. And we worked inside a large stainless steel or polyethylene pan with a half-inch of water in the bottom, which we washed thoroughly after an experiment was completed so that no residue of the solution remained around to become concentrated by evaporation.
Before a perchloric acid solution actually explodes it will start to develop a brownish-red color. Therefore we always kept a large beaker full of ice water sitting by our polishing apparatus, which we would have poured into the polishing solution to dilute and cool it if this ever happened, and we kept the door of the lab open so that we could then leave in a hurry
We stored our stock solutions in glass bottles with glass stoppers. It is important to avoid bottles with ordinary caps made of organic materials. We always rinsed the bottles thoroughly with water, to remove any traces of solution that might have dribbled down the sides of them during solution transfer. With these precautions we stored perchloric acid solutions of all kinds for many months with no problems.
Perchloric acid is a very useful reagent if you use it with proper precautions and due respect. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
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==============================Original Headers============================== 18, 22 -- From walck-at-southbaytech.com Tue Dec 11 16:03:26 2007 18, 22 -- Received: from flpi185.prodigy.net (flpi185.sbcis.sbc.com [207.115.20.187]) 18, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBBM3Ppj017048 18, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Dec 2007 16:03:25 -0600 18, 22 -- X-ORBL: [64.169.217.123] 18, 22 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) 18, 22 -- by flpi185.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id lBBM353h019226; 18, 22 -- Tue, 11 Dec 2007 14:03:12 -0800 18, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} 18, 22 -- To: {bigelow-at-umich.edu} , {Microscopy-at-microscopy.com} 18, 22 -- Subject: RE: [Microscopy] RE: Perchloric solutions 18, 22 -- Date: Tue, 11 Dec 2007 14:01:21 -0800 18, 22 -- Message-ID: {031901c83c41$69c9e900$7801a8c0-at-dynamicbl8uno3} 18, 22 -- MIME-Version: 1.0 18, 22 -- Content-Type: text/plain; 18, 22 -- charset="iso-8859-1" 18, 22 -- X-Mailer: Microsoft Office Outlook 11 18, 22 -- Thread-Index: Acg8Px+NLfU6YiV5RluqZdPqwzhN2QAAWB6Q 18, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 18, 22 -- In-Reply-To: {200712112145.lBBLjDug009615-at-ns.microscopy.com} 18, 22 -- Content-Transfer-Encoding: 8bit 18, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lBBM3Ppj017048 ==============================End of - Headers==============================
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Email: walter.bobrowski-at-pfizer.com Name: Walter Bobrowski
Organization: Pfizer Global R&D
Title-Subject: [Filtered] Advice on Imaging C60 Aggregates in Buffer Medium
Question: Any advice on preparation and imaging of C60 (fullerene) aggregates in buffer for TEM appreciated. A paper (Environ. Sci. Technol. 2005, 39, 4307-4316) states they form crystalline aggregates of roundish to rectangular shapes, 25-500nm in size. Technique has been to apply droplet to filmed grid support, wick and air dry. I'm getting crystalline aggregates in the samples preps -- as well as similar looking crystals in the buffer prep alone. TIA,
Walter F. Bobrowski Sr. Scientist Pfizer Global R&D 224 Eastern Point Rd B274/0357N Groton, CT 06340
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Email: dietz-at-anl.gov Name: N. Rago
Title-Subject: [Filtered] SEM/TEM of hydrogels
Question: I need to see the distribution of nanoparticles ( {50 nm) in a hydrogel. What is the best way to prepare these samples for SEM or TEM?
The LSM510 serie introduced the AOTF, which allows the ultra-fast switching between different incident wavelengthes. This means that if you are scanning triple-stained samples, all 3 wavelengthes are scanned sequentially (time-resolved). Well sequential scanning is not a revolution, the key here is the ultra-fast switching, which allows quasi-simultaneous ("real-time" for your eyes) detection of different wavelengthes without the chance of cross-talk. Now I did some research on the net and I found that Leica developped the same feature since 2002, although it is called AOBS ;-). I don't know if both systems all fully comparable though.
Best regards,
Stephane, male (and a nice specimen, too ;-))
----- Original Message ---- X-from: "PhillipsT-at-missouri.edu" {PhillipsT-at-missouri.edu} To: nizets2-at-yahoo.com Sent: Monday, December 10, 2007 10:00:41 PM
I own a Zeiss 510 Meta NLO and am afraid I don't understand Stephane's comment that the Zeiss separates the signal based on time. Perhaps she means that the system can, in real-time, separate out overlapping emission signals using spectral imaging. If not, perhaps she could expand on her comment.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: sekkio-at-mac.com [mailto:sekkio-at-mac.com] Sent: Monday, December 10, 2007 2:28 PM To: Phillips, Thomas E.
About multiple fluorescence we got excellent results using a white light laser coupled to the SP5AOBS I think WLL is really a great advance in spectral imaging icluding related applications ciao Alby Il giorno 20/nov/07, alle ore 15:42, nizets2-at-yahoo.com ha scritto:
} } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Hi! } } I have used the previous Zeiss 510 microscope and liked it very } much. It is user-friendly and powerful. } I don't know the technical details of the Leica but in the Zeiss the } different wavelengthes are separated not only by filters but also in } time, so no read-through is possible. I must say this gives a } welcome level of confidence and comfort. I did a lot of triple } staining with great results. } } No interest blabla.... } } Regards, } Stephane } } } ----- Original Message ---- } X-from: "christopher.hayden-at-novartis.com" {christopher.hayden-at-novartis.com } } } To: nizets2-at-yahoo.com } Sent: Monday, November 19, 2007 7:28:16 PM } Subject: [Microscopy] LM - Confocal Opinions } } } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Good Day, All: } } We're in the fortunate position of looking around at } acquiring a } confocal microscope sometime in 2008. Before we start hearing the } pitches } from the manufacturers, we'd like to hear from the "real" world. } Specifically, what you have and what you think of it (ease-of-use, } upgrades, level of control, service and support, etc.). } After having a look around, it seems like the two contenders } for } us are the Zeiss 510 META NLO and the Leica TCS SP5. Flexibility is } key } for us, as it's going to have a fairly broad set of uses as both a } research tool and as a development tool. } Cost isn't critical at this point; we're basically being told } to } "get what we need". While we have people with confocal experience, } each is } } a little biased that the system they know is the best one. } } Thanks much! } -Chris } } } } ==============================Original } Headers============================== } 5, 29 -- From christopher.hayden-at-novartis.com Mon Nov 19 12:23:16 2007 } 5, 29 -- Received: from mail194.messagelabs.com } (mail194.messagelabs.com [85.158.140.211]) } 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id lAJINGAI008522 } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } 12:23:16 -0600 } 5, 29 -- X-VirusChecked: Checked } 5, 29 -- X-Env-Sender: christopher.hayden-at-novartis.com } 5, 29 -- X-Msg-Ref: server-10.tower-194.messagelabs.com!1195496595! } 1972703!1 } 5, 29 -- X-StarScan-Version: 5.5.12.14.2; banners=-,-,- } 5, 29 -- X-Originating-IP: [160.62.1.174] } 5, 29 -- Received: (qmail 15936 invoked from network); 19 Nov 2007 } 18:23:15 -0000 } 5, 29 -- Received: from ch2ssaenov01.novartis.com (HELO } ch2ssaenov01.novartis.com) (160.62.1.174) } 5, 29 -- by server-10.tower-194.messagelabs.com with AES256-SHA } encrypted SMTP; 19 Nov 2007 18:23:15 -0000 } 5, 29 -- Received: from ch1ssainov01.novartis.net (ch1ssainov01s } [192.37.2.173]) } 5, 29 -- by ch2ssaenov01.novartis.com (8.13.8/8.13.8) with ESMTP } id lAJINE00017372 } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } 19:23:14 +0100 } 5, 29 -- Received: from phchbs-s3140.EU.novartis.net (phchbs- } s3140.eu.novartis.net [192.37.31.248]) } 5, 29 -- by ch1ssainov01.novartis.net (8.13.8/8.13.8) with ESMTP } id lAJINE7X016212 } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } 19:23:14 +0100 } 5, 29 -- To: Microscopy-at-Microscopy.Com } 5, 29 -- Subject: LM - Confocal Opinions } 5, 29 -- MIME-Version: 1.0 } 5, 29 -- X-Mailer: Lotus Notes Release 7.0.1 HF517 January 12, 2007 } 5, 29 -- Message-ID: {OFC44BD91C.2AD7BFB4-ON85257398.0064ECC4-85257398.006500CF-at-ah.novartis.c om } } } 5, 29 -- From: christopher.hayden-at-novartis.com } 5, 29 -- Date: Mon, 19 Nov 2007 13:23:13 -0500 } 5, 29 -- X-MIMETrack: Serialize by Router on CHBSSPH0/PH/ } Novartis(Release 7.0.2FP2 HF144|July 27, 2007) at } 5, 29 -- 19.11.2007 19:23:14, } 5, 29 -- Serialize complete at 19.11.2007 19:23:14 } 5, 29 -- Content-Type: text/plain; charset="US-ASCII" } ==============================End of - } Headers============================== } } } } ________________________________________________________________________ ____________ } Be a better pen pal. } Text or chat with friends inside Yahoo! 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==============================Original Headers============================== 17, 26 -- From PhillipsT-at-missouri.edu Mon Dec 10 14:55:06 2007 17, 26 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBAKt6tE020017 17, 26 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 10 Dec 2007 14:55:06 -0600 17, 26 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 17, 26 -- Mon, 10 Dec 2007 14:55:06 -0600 17, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 26 -- Content-class: urn:content-classes:message 17, 26 -- MIME-Version: 1.0 17, 26 -- Content-Type: text/plain; 17, 26 -- charset="us-ascii" 17, 26 -- Subject: RE: [Microscopy] LM - Confocal Opinions 17, 26 -- Date: Mon, 10 Dec 2007 14:55:05 -0600 17, 26 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD0331F9E4-at-UM-XMAIL06.um.umsystem.edu} 17, 26 -- In-Reply-To: {200712102027.lBAKRiH7008002-at-ns.microscopy.com} 17, 26 -- X-MS-Has-Attach: 17, 26 -- X-MS-TNEF-Correlator: 17, 26 -- Thread-Topic: [Microscopy] LM - Confocal Opinions 17, 26 -- thread-index: Acg7ayAuM5lprirLTpCkeUPeEkINOwAAxEhQ 17, 26 -- References: {200712102027.lBAKRiH7008002-at-ns.microscopy.com} 17, 26 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 17, 26 -- To: {sekkio-at-mac.com} 17, 26 -- Cc: {nizets2-at-yahoo.com} , {Microscopy-at-msa.microscopy.com} 17, 26 -- X-OriginalArrivalTime: 10 Dec 2007 20:55:06.0326 (UTC) FILETIME=[F171AF60:01C83B6E] 17, 26 -- Content-Transfer-Encoding: 8bit 17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lBAKt6tE020017 ==============================End of - Headers==============================
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==============================Original Headers============================== 32, 20 -- From nizets2-at-yahoo.com Wed Dec 12 04:28:49 2007 32, 20 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138]) 32, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lBCASnC4018687 32, 20 -- for {microscopy-at-microscopy.com} ; Wed, 12 Dec 2007 04:28:49 -0600 32, 20 -- Received: (qmail 27959 invoked by uid 60001); 12 Dec 2007 10:28:48 -0000 32, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 32, 20 -- s=s1024; d=yahoo.com; 32, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 32, 20 -- b=WtBTL9t3F16zghOM+E9IilrW5WTcU2liUy85ITVHwWCZqhCmOeAbf9w7Co/KPcUZl6QsIKpl+B3w/cJZiLl3zKy5w2ZNJv7fdBLOeKdSl7C3D/3f+WOfVfMy1YyJdCLXa5Kec83ZGz2H4ck9Vim6lzbpfpn0zy4y6gI6dOKDEv4=; 32, 20 -- X-YMail-OSG: bRk1x_0VM1lgKyx8maz8YZO_uDgEzg939O8Cp_.WAcFUNKVlhgo982bwHPuC59XIJw-- 32, 20 -- Received: from [80.122.101.100] by web37406.mail.mud.yahoo.com via HTTP; Wed, 12 Dec 2007 02:28:48 PST 32, 20 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.158.1 32, 20 -- Date: Wed, 12 Dec 2007 02:28:48 -0800 (PST) 32, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 32, 20 -- Subject: Re: [Microscopy] RE: LM - Confocal Opinions 32, 20 -- To: PhillipsT-at-missouri.edu 32, 20 -- Cc: microscopy-at-microscopy.com 32, 20 -- MIME-Version: 1.0 32, 20 -- Content-Type: text/plain; charset=us-ascii 32, 20 -- Message-ID: {727356.27514.qm-at-web37406.mail.mud.yahoo.com} ==============================End of - Headers==============================
Hello all, I am working on restoring an old Unitron N-11 inverted microscope, and I was wondering if anybody out there has one or has used one.
Specifically, I would like to know if anybody has some extra parts hanging around. I would like to at least get the wiring diagram for the Xenon source, as well as find out where the overhead light would attach.
--Justin.
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Email: arrowood-at-utep.edu Name: Roy Arrowood
Organization: University of Texas El Paso
Title-Subject: [Filtered] perchloric acid hazards
Question: To elaborate slightly on one of the points about perchloric hazards: many metal perchlorates are explosive when dry (including nickel perchlorate). So even if an organic substance is not present, there can still be serious hazards. When using a perchloric-based electrolyte to prepare superalloys for microscopy, one should take care to avoid letting used electrolyte evaporate to leave a dry deposit. Also, perchloric fumes may react with metal ductwork some distance away from the fume hood, producing explosive salts. Remodelling workers have been blinded or maimed simply by tapping on a fume hood duct which was not perchloric-rated, but had been used for perchloric-acid work. If you've not had experience with this stuff, it's wise to take some time for reading and self-education before working with perchloric!
HI Stephan, I think there is a little bit of confusion. The AOBS is a programmable beamsplitter (BS) and is, let me say, more evolute than the AOTF (about this I remember that it was introduced by Leica on 1993 approx... I had one Leica CLSM in 1987 and they told us about this new ugrade, well I am niot sure). More recently a AOBM - beam modulator has been introduced for delivering selected multiple wavelengths coming from a white light laser. This sound to be a sort of "best couple", AOBM+white light laser. AOBS has an important impact in wavelength selection in the detection scheme. It has to be carefully aligned and then it prevents from undesired reflections of the selected excitation wavelength a sort of paso doble, a gentleman and a lady dancing a Flamenco around an imaginary Bull like in a Corrida game... sorry for this... I think that the introduction of AOBS was a sort of puting brain instead of muscles in fluorescence microscopy. All my best Alby
Il giorno 12/dic/07, alle ore 11:30, nizets2-at-yahoo.com ha scritto:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Good morning! } } The LSM510 serie introduced the AOTF, which allows the ultra-fast } switching between different incident wavelengthes. } This means that if you are scanning triple-stained samples, all 3 } wavelengthes are scanned sequentially (time-resolved). Well } sequential scanning is not a revolution, the key here is the ultra- } fast switching, which allows quasi-simultaneous ("real-time" for } your eyes) detection of different wavelengthes without the chance of } cross-talk. } Now I did some research on the net and I found that Leica developped } the same feature since 2002, although it is called AOBS ;-). I don't } know if both systems all fully comparable though. } } Best regards, } } Stephane, male (and a nice specimen, too ;-)) } } } } ----- Original Message ---- } X-from: "PhillipsT-at-missouri.edu" {PhillipsT-at-missouri.edu} } To: nizets2-at-yahoo.com } Sent: Monday, December 10, 2007 10:00:41 PM } Subject: [Microscopy] RE: LM - Confocal Opinions } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I own a Zeiss 510 Meta NLO and am afraid I don't understand Stephane's } comment that the Zeiss separates the signal based on time. Perhaps } she } means that the system can, in real-time, separate out overlapping } emission signals using spectral imaging. If not, perhaps she could } expand on her comment. } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } -----Original Message----- } X-from: sekkio-at-mac.com [mailto:sekkio-at-mac.com] } Sent: Monday, December 10, 2007 2:28 PM } To: Phillips, Thomas E. } Subject: [Microscopy] LM - Confocal Opinions } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } About multiple fluorescence we got excellent results using a white } light laser coupled to the SP5AOBS } I think WLL is really a great advance in spectral imaging icluding } related applications } ciao } Alby } Il giorno 20/nov/07, alle ore 15:42, nizets2-at-yahoo.com ha scritto: } } } } } } } } } } ------------------------------------------------------------------------ } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------ } ---- } } } } Hi! } } } } I have used the previous Zeiss 510 microscope and liked it very } } much. It is user-friendly and powerful. } } I don't know the technical details of the Leica but in the Zeiss the } } different wavelengthes are separated not only by filters but also in } } time, so no read-through is possible. I must say this gives a } } welcome level of confidence and comfort. I did a lot of triple } } staining with great results. } } } } No interest blabla.... } } } } Regards, } } Stephane } } } } } } ----- Original Message ---- } } X-from: "christopher.hayden-at-novartis.com" } {christopher.hayden-at-novartis.com } } } } } To: nizets2-at-yahoo.com } } Sent: Monday, November 19, 2007 7:28:16 PM } } Subject: [Microscopy] LM - Confocal Opinions } } } } } } } } } } } ------------------------------------------------------------------------ } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------ } ---- } } } } Good Day, All: } } } } We're in the fortunate position of looking around at } } acquiring a } } confocal microscope sometime in 2008. Before we start hearing the } } pitches } } from the manufacturers, we'd like to hear from the "real" world. } } Specifically, what you have and what you think of it (ease-of-use, } } upgrades, level of control, service and support, etc.). } } After having a look around, it seems like the two contenders } } for } } us are the Zeiss 510 META NLO and the Leica TCS SP5. Flexibility is } } key } } for us, as it's going to have a fairly broad set of uses as both a } } research tool and as a development tool. } } Cost isn't critical at this point; we're basically being told } } to } } "get what we need". While we have people with confocal experience, } } each is } } } } a little biased that the system they know is the best one. } } } } Thanks much! } } -Chris } } } } } } } } ==============================Original } } Headers============================== } } 5, 29 -- From christopher.hayden-at-novartis.com Mon Nov 19 12:23:16 } } 2007 } } 5, 29 -- Received: from mail194.messagelabs.com } } (mail194.messagelabs.com [85.158.140.211]) } } 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id lAJINGAI008522 } } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } } 12:23:16 -0600 } } 5, 29 -- X-VirusChecked: Checked } } 5, 29 -- X-Env-Sender: christopher.hayden-at-novartis.com } } 5, 29 -- X-Msg-Ref: server-10.tower-194.messagelabs.com!1195496595! } } 1972703!1 } } 5, 29 -- X-StarScan-Version: 5.5.12.14.2; banners=-,-,- } } 5, 29 -- X-Originating-IP: [160.62.1.174] } } 5, 29 -- Received: (qmail 15936 invoked from network); 19 Nov 2007 } } 18:23:15 -0000 } } 5, 29 -- Received: from ch2ssaenov01.novartis.com (HELO } } ch2ssaenov01.novartis.com) (160.62.1.174) } } 5, 29 -- by server-10.tower-194.messagelabs.com with AES256-SHA } } encrypted SMTP; 19 Nov 2007 18:23:15 -0000 } } 5, 29 -- Received: from ch1ssainov01.novartis.net (ch1ssainov01s } } [192.37.2.173]) } } 5, 29 -- by ch2ssaenov01.novartis.com (8.13.8/8.13.8) with ESMTP } } id lAJINE00017372 } } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } } 19:23:14 +0100 } } 5, 29 -- Received: from phchbs-s3140.EU.novartis.net (phchbs- } } s3140.eu.novartis.net [192.37.31.248]) } } 5, 29 -- by ch1ssainov01.novartis.net (8.13.8/8.13.8) with ESMTP } } id lAJINE7X016212 } } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } } 19:23:14 +0100 } } 5, 29 -- To: Microscopy-at-Microscopy.Com } } 5, 29 -- Subject: LM - Confocal Opinions } } 5, 29 -- MIME-Version: 1.0 } } 5, 29 -- X-Mailer: Lotus Notes Release 7.0.1 HF517 January 12, 2007 } } 5, 29 -- Message-ID: } {OFC44BD91C.2AD7BFB4-ON85257398.0064ECC4-85257398.006500CF-at-ah.novartis.c } om } } } } } 5, 29 -- From: christopher.hayden-at-novartis.com } } 5, 29 -- Date: Mon, 19 Nov 2007 13:23:13 -0500 } } 5, 29 -- X-MIMETrack: Serialize by Router on CHBSSPH0/PH/ } } Novartis(Release 7.0.2FP2 HF144|July 27, 2007) at } } 5, 29 -- 19.11.2007 19:23:14, } } 5, 29 -- Serialize complete at 19.11.2007 19:23:14 } } 5, 29 -- Content-Type: text/plain; charset="US-ASCII" } } ==============================End of - } } Headers============================== } } } } } } } } } ________________________________________________________________________ } ____________ } } Be a better pen pal. } } Text or chat with friends inside Yahoo! Mail. See how. } http://overview.mail.yahoo.com/ } } } } ==============================Original } } Headers============================== } } 19, 20 -- From nizets2-at-yahoo.com Tue Nov 20 08:39:39 2007 } } 19, 20 -- Received: from web37414.mail.mud.yahoo.com } } (web37414.mail.mud.yahoo.com [209.191.91.146]) } } 19, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
No problem for the gender, it is the same problem everywhere from germany to USA :-)
A microscope which scans and has a pinhole is indeed called Confocal Laser Scanning Microscope (confocal LSM). The possibility to select a certain incoming wavelength is just a useful feature, but not the basic principle of the microscope. This way you can be certain that the second dye is not excited by the excitation wv of the first dye. Perhaps I misused the word "time-resolved", since english is not my primary language. However, this is indeed a sequential scanning in a confocal microscope. Please note that I always carefully noted that this feature is useful for multiple labelings. It is useless for single labelings.
Regards, Stephane
----- Original Message ---- X-from: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} To: nizets2-at-yahoo.com Sent: Wednesday, December 12, 2007 4:14:47 PM
Good morning!
The LSM510 serie introduced the AOTF, which allows the ultra-fast switching between different incident wavelengthes. This means that if you are scanning triple-stained samples, all 3 wavelengthes are scanned sequentially (time-resolved). Well sequential scanning is not a revolution, the key here is the ultra-fast switching, which allows quasi-simultaneous ("real-time" for your eyes) detection of different wavelengthes without the chance of cross-talk. Now I did some research on the net and I found that Leica developped the same feature since 2002, although it is called AOBS ;-). I don't know if both systems all fully comparable though.
Best regards,
Stephane, male (and a nice specimen, too ;-))
----- Original Message ---- X-from: "PhillipsT-at-missouri.edu" {PhillipsT-at-missouri.edu} To: nizets2-at-yahoo.com Sent: Monday, December 10, 2007 10:00:41 PM
I own a Zeiss 510 Meta NLO and am afraid I don't understand Stephane's comment that the Zeiss separates the signal based on time. Perhaps she means that the system can, in real-time, separate out overlapping emission signals using spectral imaging. If not, perhaps she could expand on her comment.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: sekkio-at-mac.com [mailto:sekkio-at-mac.com] Sent: Monday, December 10, 2007 2:28 PM To: Phillips, Thomas E.
About multiple fluorescence we got excellent results using a white light laser coupled to the SP5AOBS I think WLL is really a great advance in spectral imaging icluding related applications ciao Alby Il giorno 20/nov/07, alle ore 15:42, nizets2-at-yahoo.com ha scritto:
} } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Hi! } } I have used the previous Zeiss 510 microscope and liked it very } much. It is user-friendly and powerful. } I don't know the technical details of the Leica but in the Zeiss the } different wavelengthes are separated not only by filters but also in } time, so no read-through is possible. I must say this gives a } welcome level of confidence and comfort. I did a lot of triple } staining with great results. } } No interest blabla.... } } Regards, } Stephane } } } ----- Original Message ---- } X-from: "christopher.hayden-at-novartis.com" {christopher.hayden-at-novartis.com } } } To: nizets2-at-yahoo.com } Sent: Monday, November 19, 2007 7:28:16 PM } Subject: [Microscopy] LM - Confocal Opinions } } } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Good Day, All: } } We're in the fortunate position of looking around at } acquiring a } confocal microscope sometime in 2008. Before we start hearing the } pitches } from the manufacturers, we'd like to hear from the "real" world. } Specifically, what you have and what you think of it (ease-of-use, } upgrades, level of control, service and support, etc.). } After having a look around, it seems like the two contenders } for } us are the Zeiss 510 META NLO and the Leica TCS SP5. Flexibility is } key } for us, as it's going to have a fairly broad set of uses as both a } research tool and as a development tool. } Cost isn't critical at this point; we're basically being told } to } "get what we need". While we have people with confocal experience, } each is } } a little biased that the system they know is the best one. } } Thanks much! } -Chris } } } } ==============================Original } Headers============================== } 5, 29 -- From christopher.hayden-at-novartis.com Mon Nov 19 12:23:16 2007 } 5, 29 -- Received: from mail194.messagelabs.com } (mail194.messagelabs.com [85.158.140.211]) } 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id lAJINGAI008522 } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } 12:23:16 -0600 } 5, 29 -- X-VirusChecked: Checked } 5, 29 -- X-Env-Sender: christopher.hayden-at-novartis.com } 5, 29 -- X-Msg-Ref: server-10.tower-194.messagelabs.com!1195496595! } 1972703!1 } 5, 29 -- X-StarScan-Version: 5.5.12.14.2; banners=-,-,- } 5, 29 -- X-Originating-IP: [160.62.1.174] } 5, 29 -- Received: (qmail 15936 invoked from network); 19 Nov 2007 } 18:23:15 -0000 } 5, 29 -- Received: from ch2ssaenov01.novartis.com (HELO } ch2ssaenov01.novartis.com) (160.62.1.174) } 5, 29 -- by server-10.tower-194.messagelabs.com with AES256-SHA } encrypted SMTP; 19 Nov 2007 18:23:15 -0000 } 5, 29 -- Received: from ch1ssainov01.novartis.net (ch1ssainov01s } [192.37.2.173]) } 5, 29 -- by ch2ssaenov01.novartis.com (8.13.8/8.13.8) with ESMTP } id lAJINE00017372 } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } 19:23:14 +0100 } 5, 29 -- Received: from phchbs-s3140.EU.novartis.net (phchbs- } s3140.eu.novartis.net [192.37.31.248]) } 5, 29 -- by ch1ssainov01.novartis.net (8.13.8/8.13.8) with ESMTP } id lAJINE7X016212 } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } 19:23:14 +0100 } 5, 29 -- To: Microscopy-at-Microscopy.Com } 5, 29 -- Subject: LM - Confocal Opinions } 5, 29 -- MIME-Version: 1.0 } 5, 29 -- X-Mailer: Lotus Notes Release 7.0.1 HF517 January 12, 2007 } 5, 29 -- Message-ID: {OFC44BD91C.2AD7BFB4-ON85257398.0064ECC4-85257398.006500CF-at-ah.novartis.c om } } } 5, 29 -- From: christopher.hayden-at-novartis.com } 5, 29 -- Date: Mon, 19 Nov 2007 13:23:13 -0500 } 5, 29 -- X-MIMETrack: Serialize by Router on CHBSSPH0/PH/ } Novartis(Release 7.0.2FP2 HF144|July 27, 2007) at } 5, 29 -- 19.11.2007 19:23:14, } 5, 29 -- Serialize complete at 19.11.2007 19:23:14 } 5, 29 -- Content-Type: text/plain; charset="US-ASCII" } ==============================End of - } Headers============================== } } } } ________________________________________________________________________ ____________ } Be a better pen pal. } Text or chat with friends inside Yahoo! Mail. See how. http://overview.mail.yahoo.com/ } } ==============================Original } Headers============================== } 19, 20 -- From nizets2-at-yahoo.com Tue Nov 20 08:39:39 2007 } 19, 20 -- Received: from web37414.mail.mud.yahoo.com } (web37414.mail.mud.yahoo.com [209.191.91.146]) } 19, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP
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==============================Original Headers============================== 17, 26 -- From PhillipsT-at-missouri.edu Mon Dec 10 14:55:06 2007 17, 26 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBAKt6tE020017 17, 26 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 10 Dec 2007 14:55:06 -0600 17, 26 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 17, 26 -- Mon, 10 Dec 2007 14:55:06 -0600 17, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 26 -- Content-class: urn:content-classes:message 17, 26 -- MIME-Version: 1.0 17, 26 -- Content-Type: text/plain; 17, 26 -- charset="us-ascii" 17, 26 -- Subject: RE: [Microscopy] LM - Confocal Opinions 17, 26 -- Date: Mon, 10 Dec 2007 14:55:05 -0600 17, 26 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD0331F9E4-at-UM-XMAIL06.um.umsystem.edu} 17, 26 -- In-Reply-To: {200712102027.lBAKRiH7008002-at-ns.microscopy.com} 17, 26 -- X-MS-Has-Attach: 17, 26 -- X-MS-TNEF-Correlator: 17, 26 -- Thread-Topic: [Microscopy] LM - Confocal Opinions 17, 26 -- thread-index: Acg7ayAuM5lprirLTpCkeUPeEkINOwAAxEhQ 17, 26 -- References: {200712102027.lBAKRiH7008002-at-ns.microscopy.com} 17, 26 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 17, 26 -- To: {sekkio-at-mac.com} 17, 26 -- Cc: {nizets2-at-yahoo.com} , {Microscopy-at-msa.microscopy.com} 17, 26 -- X-OriginalArrivalTime: 10 Dec 2007 20:55:06.0326 (UTC) FILETIME=[F171AF60:01C83B6E] 17, 26 -- Content-Transfer-Encoding: 8bit 17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lBAKt6tE020017 ==============================End of - Headers==============================
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==============================Original Headers============================== 32, 20 -- From nizets2-at-yahoo.com Wed Dec 12 04:28:49 2007 32, 20 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138]) 32, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lBCASnC4018687 32, 20 -- for {microscopy-at-microscopy.com} ; Wed, 12 Dec 2007 04:28:49 -0600 32, 20 -- Received: (qmail 27959 invoked by uid 60001); 12 Dec 2007 10:28:48 -0000 32, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 32, 20 -- s=s1024; d=yahoo.com; 32, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 32, 20 -- b=WtBTL9t3F16zghOM+E9IilrW5WTcU2liUy85ITVHwWCZqhCmOeAbf9w7Co/KPcUZl6QsIKpl+B3w/cJZiLl3zKy5w2ZNJv7fdBLOeKdSl7C3D/3f+WOfVfMy1YyJdCLXa5Kec83ZGz2H4ck9Vim6lzbpfpn0zy4y6gI6dOKDEv4=; 32, 20 -- X-YMail-OSG: bRk1x_0VM1lgKyx8maz8YZO_uDgEzg939O8Cp_.WAcFUNKVlhgo982bwHPuC59XIJw-- 32, 20 -- Received: from [80.122.101.100] by web37406.mail.mud.yahoo.com via HTTP; Wed, 12 Dec 2007 02:28:48 PST 32, 20 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.158.1 32, 20 -- Date: Wed, 12 Dec 2007 02:28:48 -0800 (PST) 32, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 32, 20 -- Subject: Re: [Microscopy] RE: LM - Confocal Opinions 32, 20 -- To: PhillipsT-at-missouri.edu 32, 20 -- Cc: microscopy-at-microscopy.com 32, 20 -- MIME-Version: 1.0 32, 20 -- Content-Type: text/plain; charset=us-ascii 32, 20 -- Message-ID: {727356.27514.qm-at-web37406.mail.mud.yahoo.com} ==============================End of - Headers==============================
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==============================Original Headers============================== 52, 20 -- From nizets2-at-yahoo.com Thu Dec 13 05:49:14 2007 52, 20 -- Received: from web37411.mail.mud.yahoo.com (web37411.mail.mud.yahoo.com [209.191.91.143]) 52, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lBDBnEwZ011701 52, 20 -- for {microscopy-at-microscopy.com} ; Thu, 13 Dec 2007 05:49:14 -0600 52, 20 -- Received: (qmail 19904 invoked by uid 60001); 13 Dec 2007 11:49:13 -0000 52, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 52, 20 -- s=s1024; d=yahoo.com; 52, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 52, 20 -- b=DhANdpVGhx7Bl4fEjnun/MApF+VoJCirgqN1Qtf+P/3szoKSa4CBSHsw1zR5XljzUWJXprd6OhDPfURbIuYgK/5o25q6UhnIAdZk5C88mtu8dFNdGliICujpZQY9/08tIANbYS6kzHSZqDyq1vHKZmW88JRcNYnHCbPK/mN5gTo=; 52, 20 -- X-YMail-OSG: vaCR5rMVM1mldtmAMHNb65y0k2_nkUy6zGwRnkI3fHBNterKS.81Xzkko0EXiQynJvYH.qw98uNhHN5KT2BRstmr2lOSftzFhkqTzcGLKpLO8rkiR5qeVT2_eKrUcUgthBrB5qrrfMqryO2O4jgQj8POyp.eA_y93I9mnOPg_PbG 52, 20 -- Received: from [80.122.101.100] by web37411.mail.mud.yahoo.com via HTTP; Thu, 13 Dec 2007 03:49:13 PST 52, 20 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.158.1 52, 20 -- Date: Thu, 13 Dec 2007 03:49:13 -0800 (PST) 52, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 52, 20 -- Subject: Re: [Microscopy] LM - Confocal Opinions 52, 20 -- To: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 52, 20 -- Cc: microscopy-at-microscopy.com 52, 20 -- MIME-Version: 1.0 52, 20 -- Content-Type: text/plain; charset=us-ascii 52, 20 -- Message-ID: {842796.19359.qm-at-web37411.mail.mud.yahoo.com} ==============================End of - Headers==============================
Thank you for your remark. As far as I understand the AOBS allows the selection of specific excitation wv, just like the AOTF (although the principles are clearly different). It is said that you can use the system for sequential scanning, just like the AOTF. I paste here a link to a brochure from Leica which describes sequential scanning. Please note that the first chapter is called "avoiding crosstalk", which was precisely the subject of my note.
Perhaps I am missing something (I am not working with confocal microscopes anymore for years). In this case, please correct me.
regards,
Stephane
----- Original Message ---- X-from: "sekkio-at-mac.com" {sekkio-at-mac.com} To: nizets2-at-yahoo.com Sent: Thursday, December 13, 2007 8:05:03 AM
HI Stephan, I think there is a little bit of confusion. The AOBS is a programmable beamsplitter (BS) and is, let me say, more evolute than the AOTF (about this I remember that it was introduced by Leica on 1993 approx... I had one Leica CLSM in 1987 and they told us about this new ugrade, well I am niot sure). More recently a AOBM - beam modulator has been introduced for delivering selected multiple wavelengths coming from a white light laser. This sound to be a sort of "best couple", AOBM+white light laser. AOBS has an important impact in wavelength selection in the detection scheme. It has to be carefully aligned and then it prevents from undesired reflections of the selected excitation wavelength a sort of paso doble, a gentleman and a lady dancing a Flamenco around an imaginary Bull like in a Corrida game... sorry for this... I think that the introduction of AOBS was a sort of puting brain instead of muscles in fluorescence microscopy. All my best Alby
Il giorno 12/dic/07, alle ore 11:30, nizets2-at-yahoo.com ha scritto:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Good morning! } } The LSM510 serie introduced the AOTF, which allows the ultra-fast } switching between different incident wavelengthes. } This means that if you are scanning triple-stained samples, all 3 } wavelengthes are scanned sequentially (time-resolved). Well } sequential scanning is not a revolution, the key here is the ultra- } fast switching, which allows quasi-simultaneous ("real-time" for } your eyes) detection of different wavelengthes without the chance of } cross-talk. } Now I did some research on the net and I found that Leica developped } the same feature since 2002, although it is called AOBS ;-). I don't } know if both systems all fully comparable though. } } Best regards, } } Stephane, male (and a nice specimen, too ;-)) } } } } ----- Original Message ---- } X-from: "PhillipsT-at-missouri.edu" {PhillipsT-at-missouri.edu} } To: nizets2-at-yahoo.com } Sent: Monday, December 10, 2007 10:00:41 PM } Subject: [Microscopy] RE: LM - Confocal Opinions } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I own a Zeiss 510 Meta NLO and am afraid I don't understand Stephane's } comment that the Zeiss separates the signal based on time. Perhaps } she } means that the system can, in real-time, separate out overlapping } emission signals using spectral imaging. If not, perhaps she could } expand on her comment. } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } -----Original Message----- } X-from: sekkio-at-mac.com [mailto:sekkio-at-mac.com] } Sent: Monday, December 10, 2007 2:28 PM } To: Phillips, Thomas E. } Subject: [Microscopy] LM - Confocal Opinions } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } About multiple fluorescence we got excellent results using a white } light laser coupled to the SP5AOBS } I think WLL is really a great advance in spectral imaging icluding } related applications } ciao } Alby } Il giorno 20/nov/07, alle ore 15:42, nizets2-at-yahoo.com ha scritto: } } } } } } } } } } ------------------------------------------------------------------------ } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------ } ---- } } } } Hi! } } } } I have used the previous Zeiss 510 microscope and liked it very } } much. It is user-friendly and powerful. } } I don't know the technical details of the Leica but in the Zeiss the } } different wavelengthes are separated not only by filters but also in } } time, so no read-through is possible. I must say this gives a } } welcome level of confidence and comfort. I did a lot of triple } } staining with great results. } } } } No interest blabla.... } } } } Regards, } } Stephane } } } } } } ----- Original Message ---- } } X-from: "christopher.hayden-at-novartis.com" } {christopher.hayden-at-novartis.com } } } } } To: nizets2-at-yahoo.com } } Sent: Monday, November 19, 2007 7:28:16 PM } } Subject: [Microscopy] LM - Confocal Opinions } } } } } } } } } } } ------------------------------------------------------------------------ } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------ } ---- } } } } Good Day, All: } } } } We're in the fortunate position of looking around at } } acquiring a } } confocal microscope sometime in 2008. Before we start hearing the } } pitches } } from the manufacturers, we'd like to hear from the "real" world. } } Specifically, what you have and what you think of it (ease-of-use, } } upgrades, level of control, service and support, etc.). } } After having a look around, it seems like the two contenders } } for } } us are the Zeiss 510 META NLO and the Leica TCS SP5. Flexibility is } } key } } for us, as it's going to have a fairly broad set of uses as both a } } research tool and as a development tool. } } Cost isn't critical at this point; we're basically being told } } to } } "get what we need". While we have people with confocal experience, } } each is } } } } a little biased that the system they know is the best one. } } } } Thanks much! } } -Chris } } } } } } } } ==============================Original } } Headers============================== } } 5, 29 -- From christopher.hayden-at-novartis.com Mon Nov 19 12:23:16 } } 2007 } } 5, 29 -- Received: from mail194.messagelabs.com } } (mail194.messagelabs.com [85.158.140.211]) } } 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id lAJINGAI008522 } } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } } 12:23:16 -0600 } } 5, 29 -- X-VirusChecked: Checked } } 5, 29 -- X-Env-Sender: christopher.hayden-at-novartis.com } } 5, 29 -- X-Msg-Ref: server-10.tower-194.messagelabs.com!1195496595! } } 1972703!1 } } 5, 29 -- X-StarScan-Version: 5.5.12.14.2; banners=-,-,- } } 5, 29 -- X-Originating-IP: [160.62.1.174] } } 5, 29 -- Received: (qmail 15936 invoked from network); 19 Nov 2007 } } 18:23:15 -0000 } } 5, 29 -- Received: from ch2ssaenov01.novartis.com (HELO } } ch2ssaenov01.novartis.com) (160.62.1.174) } } 5, 29 -- by server-10.tower-194.messagelabs.com with AES256-SHA } } encrypted SMTP; 19 Nov 2007 18:23:15 -0000 } } 5, 29 -- Received: from ch1ssainov01.novartis.net (ch1ssainov01s } } [192.37.2.173]) } } 5, 29 -- by ch2ssaenov01.novartis.com (8.13.8/8.13.8) with ESMTP } } id lAJINE00017372 } } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } } 19:23:14 +0100 } } 5, 29 -- Received: from phchbs-s3140.EU.novartis.net (phchbs- } } s3140.eu.novartis.net [192.37.31.248]) } } 5, 29 -- by ch1ssainov01.novartis.net (8.13.8/8.13.8) with ESMTP } } id lAJINE7X016212 } } 5, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 Nov 2007 } } 19:23:14 +0100 } } 5, 29 -- To: Microscopy-at-Microscopy.Com } } 5, 29 -- Subject: LM - Confocal Opinions } } 5, 29 -- MIME-Version: 1.0 } } 5, 29 -- X-Mailer: Lotus Notes Release 7.0.1 HF517 January 12, 2007 } } 5, 29 -- Message-ID: } {OFC44BD91C.2AD7BFB4-ON85257398.0064ECC4-85257398.006500CF-at-ah.novartis.c } om } } } } } 5, 29 -- From: christopher.hayden-at-novartis.com } } 5, 29 -- Date: Mon, 19 Nov 2007 13:23:13 -0500 } } 5, 29 -- X-MIMETrack: Serialize by Router on CHBSSPH0/PH/ } } Novartis(Release 7.0.2FP2 HF144|July 27, 2007) at } } 5, 29 -- 19.11.2007 19:23:14, } } 5, 29 -- Serialize complete at 19.11.2007 19:23:14 } } 5, 29 -- Content-Type: text/plain; charset="US-ASCII" } } ==============================End of - } } Headers============================== } } } } } } } } } ________________________________________________________________________ } ____________ } } Be a better pen pal. } } Text or chat with friends inside Yahoo! Mail. See how. } http://overview.mail.yahoo.com/ } } } } ==============================Original } } Headers============================== } } 19, 20 -- From nizets2-at-yahoo.com Tue Nov 20 08:39:39 2007 } } 19, 20 -- Received: from web37414.mail.mud.yahoo.com } } (web37414.mail.mud.yahoo.com [209.191.91.146]) } } 19, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
I did run a Leica SP2 at my old workplace [Institute of Ophthalmology, UCL, UK], and now we use a Zeiss 510 MetaHead at the Wellcome Institute here in Oxford [UK] as our confocal. I always thought an AOBS was pretty much an AOTF - the acousto-optical thingy being the important bit (with BS = Beam Splitter, TF = Tunable Filter). e.g. http://micro.magnet.fsu.edu/primer/java/filters/aotf/index.html.
The AOBS/AOTF is standard in both these confocals (and others) to control laser power and excitation wavelength (i.e. select laser lines). To be honest it's not these rather clever excitation AOBS devices that mark these systems out (as they work very well in both), but rather the way the systems deal with the emission spectra. Both the Zeiss and the Leica have user tunable emission filters, rather than just fixed spectrum glass filters - Zeiss have the MetaHead and Leica have their patented prism tunable spectral emission filters. Leica dispense with fixed spectrum emission filters altogether with their SP confocals, and each PMT has its own tunable emission filter:
"The Leica SP5 features five confocal channels in parallel. Emission selection is done for all channels and scanning techniques by Leica's SP detectors, the only real spectral device for imaging, with tunable emission bands and maximum efficiency. Moreover, the high-transmission and tunable photongate, the Leica AOBS, fits to both scanners, avoiding all disadvantages of dichroic mirrors - there is no better way to separate excitation from emission."
With the Zeiss, the emission tunable MetaHead unit is fixed over one PMT (the other two PMTs using conventional emission filters for routine fluorochromes like DAPI, FITC, TRITC and CY5). The Zeiss offers a lot of spectral unmixing [bleedthrough] and co-localisation analysis software/hardware (as does Leica). Apparently these tunable filters are a bit more efficient (less light loss) than conventional glass filters, as well as being far more versatile.
Overall both the Leica and Zeiss confocals offer a lot for your money and you wouldn't really go wrong choosing either one. Image quality is pretty indistinguishable between them, being as much dependent on objectives etc.. as on the confocal scanning system, but both offer a lot in terms of spectral unmixing, time-lapse, 3D, FRAP, FRET etc..as well as basic confocal imaging. Just make you specify them all in the original quote as many modules/hardware are options and may be heavily discounted in the original bid (& having to find $15,000 6 months later is often a pain). I have emailed Chris separately giving my views, and I was pretty positive about both confocal systems, and their support teams, over here in the UK.
Dr Keith J Morris Molecular Cytogenetics and Microscopy Core The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom
We know accurately only when we know little, with knowledge doubt increases.
- Johann Wolfgang von Goethe c1810
Although I also rather like his quotation:
We do not have to visit a madhouse to find disordered minds; our planet is the mental institution of the universe.
-----Original Message----- X-from: sekkio-at-mac.com [mailto:sekkio-at-mac.com] Sent: 13 December 2007 07:08 To: kjmorris-at-well.ox.ac.uk
HI Stephan, I think there is a little bit of confusion. The AOBS is a programmable beamsplitter (BS) and is, let me say, more evolute than the AOTF (about this I remember that it was introduced by Leica on 1993 approx... I had one Leica CLSM in 1987 and they told us about this new ugrade, well I am niot sure). More recently a AOBM - beam modulator has been introduced for delivering selected multiple wavelengths coming from a white light laser. This sound to be a sort of "best couple", AOBM+white light laser. AOBS has an important impact in wavelength selection in the detection scheme. It has to be carefully aligned and then it prevents from undesired reflections of the selected excitation wavelength a sort of paso doble, a gentleman and a lady dancing a Flamenco around an imaginary Bull like in a Corrida game... sorry for this... I think that the introduction of AOBS was a sort of puting brain instead of muscles in fluorescence microscopy. All my best Alby
Il giorno 12/dic/07, alle ore 11:30, nizets2-at-yahoo.com ha scritto:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Good morning! } } The LSM510 serie introduced the AOTF, which allows the ultra-fast } switching between different incident wavelengthes. } This means that if you are scanning triple-stained samples, all 3 } wavelengthes are scanned sequentially (time-resolved). Well } sequential scanning is not a revolution, the key here is the ultra- } fast switching, which allows quasi-simultaneous ("real-time" for } your eyes) detection of different wavelengthes without the chance of } cross-talk. } Now I did some research on the net and I found that Leica developped } the same feature since 2002, although it is called AOBS ;-). I don't } know if both systems all fully comparable though. } } Best regards, } } Stephane, male (and a nice specimen, too ;-)) } } } } ----- Original Message ---- } X-from: "PhillipsT-at-missouri.edu" {PhillipsT-at-missouri.edu} } To: nizets2-at-yahoo.com } Sent: Monday, December 10, 2007 10:00:41 PM } Subject: [Microscopy] RE: LM - Confocal Opinions } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I own a Zeiss 510 Meta NLO and am afraid I don't understand Stephane's } comment that the Zeiss separates the signal based on time. Perhaps } she } means that the system can, in real-time, separate out overlapping } emission signals using spectral imaging. If not, perhaps she could } expand on her comment. } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } -----Original Message----- } X-from: sekkio-at-mac.com [mailto:sekkio-at-mac.com] } Sent: Monday, December 10, 2007 2:28 PM } To: Phillips, Thomas E. } Subject: [Microscopy] LM - Confocal Opinions } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } About multiple fluorescence we got excellent results using a white } light laser coupled to the SP5AOBS } I think WLL is really a great advance in spectral imaging icluding } related applications } ciao } Alby } Il giorno 20/nov/07, alle ore 15:42, nizets2-at-yahoo.com ha scritto: } } } } } } } } } } ------------------------------------------------------------------------ } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------ } ---- } } } } Hi! } } } } I have used the previous Zeiss 510 microscope and liked it very } } much. It is user-friendly and powerful. } } I don't know the technical details of the Leica but in the Zeiss the } } different wavelengthes are separated not only by filters but also in } } time, so no read-through is possible. I must say this gives a } } welcome level of confidence and comfort. I did a lot of triple } } staining with great results. } } } } No interest blabla.... } } } } Regards, } } Stephane } } } } } } ----- Original Message ---- } } X-from: "christopher.hayden-at-novartis.com" } {christopher.hayden-at-novartis.com } } } } } To: nizets2-at-yahoo.com } } Sent: Monday, November 19, 2007 7:28:16 PM } } Subject: [Microscopy] LM - Confocal Opinions } } } } } } } } } } } ------------------------------------------------------------------------ } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------ } ---- } } } } Good Day, All: } } } } We're in the fortunate position of looking around at } } acquiring a } } confocal microscope sometime in 2008. Before we start hearing the } } pitches } } from the manufacturers, we'd like to hear from the "real" world. } } Specifically, what you have and what you think of it (ease-of-use, } } upgrades, level of control, service and support, etc.). } } After having a look around, it seems like the two contenders } } for } } us are the Zeiss 510 META NLO and the Leica TCS SP5. Flexibility is } } key } } for us, as it's going to have a fairly broad set of uses as both a } } research tool and as a development tool. } } Cost isn't critical at this point; we're basically being told } } to } } "get what we need". While we have people with confocal experience, } } each is } } } } a little biased that the system they know is the best one. } } } } Thanks much! } } -Chris } } } } } } } } ==============================Original } } Headers============================== of - Headers==============================
==============================Original Headers============================== 29, 20 -- From kjmorris-at-well.ox.ac.uk Thu Dec 13 09:54:02 2007 29, 20 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 29, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBDFs1fZ010828 29, 20 -- for {Microscopy-at-Microscopy.Com} ; Thu, 13 Dec 2007 09:54:02 -0600 29, 20 -- Received: from dhcp420.well.ox.ac.uk ([129.67.46.7] helo=cyto01) 29, 20 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 29, 20 -- id 1J2qNq-00064n-A7 29, 20 -- for Microscopy-at-Microscopy.Com; Thu, 13 Dec 2007 15:53:58 +0000 29, 20 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 29, 20 -- To: {Microscopy-at-Microscopy.Com} 29, 20 -- Subject: FW: [Microscopy] Re: LM - Confocal Opinions 29, 20 -- Date: Thu, 13 Dec 2007 15:56:24 -0000 29, 20 -- Message-ID: {000001c83da0$b66c6d40$072e4381-at-cyto01} 29, 20 -- MIME-Version: 1.0 29, 20 -- Content-Type: text/plain; 29, 20 -- charset="us-ascii" 29, 20 -- Content-Transfer-Encoding: 7bit 29, 20 -- X-Mailer: Microsoft Office Outlook 11 29, 20 -- Thread-Index: Acg9Vv9QQAHkURIqTgCOM3OkSv5i+AAEq8ggAAMHsDA= 29, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
Keith does an excellent job summing up how I would respond to this discussion. The Zeiss and Leica both have similar capabilities, no matter which trademarked or otherwise created term (AOBS or AOTF) they are using to describe the functions. I recommend seeing which user format you like the best and checking reliability issues.
If you contact each vendor, they may be able to arrange for you to get some hands-on testing on some local machines. I recommend bringing your own sample, as any vendor can make their 'made to order' samples look good on their machine.
In addition, be aware that each vendor has their own special image format, which can make post-capture image viewing/processing tricky. If one vendor has a lower price, they may make up the cost later by selling licenses for the image viewing software for each station, versus one license for a whole department.
Good luck, ~Gregg
Gregg Sobocinski Imaging Specialist/Microscopist Univeristy of Michigan, MCDB (Biology) Dept. Ann Arbor, Michigan, USA
-----Original Message----- X-from: kjmorris-at-well.ox.ac.uk [mailto:kjmorris-at-well.ox.ac.uk] Sent: Thursday, December 13, 2007 11:03 AM To: Sobocinski, Gregg
Hi All,
I did run a Leica SP2 at my old workplace [Institute of Ophthalmology, UCL, UK], and now we use a Zeiss 510 MetaHead at the Wellcome Institute here in Oxford [UK] as our confocal. I always thought an AOBS was pretty much an AOTF - the acousto-optical thingy being the important bit (with BS = Beam Splitter, TF = Tunable Filter). e.g. http://micro.magnet.fsu.edu/primer/java/filters/aotf/index.html.
The AOBS/AOTF is standard in both these confocals (and others) to control laser power and excitation wavelength (i.e. select laser lines). To be honest it's not these rather clever excitation AOBS devices that mark these systems out (as they work very well in both), but rather the way the systems deal with the emission spectra. Both the Zeiss and the Leica have user tunable emission filters, rather than just fixed spectrum glass filters - Zeiss have the MetaHead and Leica have their patented prism tunable spectral emission filters. Leica dispense with fixed spectrum emission filters altogether with their SP confocals, and each PMT has its own tunable emission filter:
"The Leica SP5 features five confocal channels in parallel. Emission selection is done for all channels and scanning techniques by Leica's SP detectors, the only real spectral device for imaging, with tunable emission bands and maximum efficiency. Moreover, the high-transmission and tunable photongate, the Leica AOBS, fits to both scanners, avoiding all disadvantages of dichroic mirrors - there is no better way to separate excitation from emission."
With the Zeiss, the emission tunable MetaHead unit is fixed over one PMT (the other two PMTs using conventional emission filters for routine fluorochromes like DAPI, FITC, TRITC and CY5). The Zeiss offers a lot of spectral unmixing [bleedthrough] and co-localisation analysis software/hardware (as does Leica). Apparently these tunable filters are a bit more efficient (less light loss) than conventional glass filters, as well as being far more versatile.
Overall both the Leica and Zeiss confocals offer a lot for your money and you wouldn't really go wrong choosing either one. Image quality is pretty indistinguishable between them, being as much dependent on objectives etc.. as on the confocal scanning system, but both offer a lot in terms of spectral unmixing, time-lapse, 3D, FRAP, FRET etc..as well as basic confocal imaging. Just make you specify them all in the original quote as many modules/hardware are options and may be heavily discounted in the original bid (& having to find $15,000 6 months later is often a pain). I have emailed Chris separately giving my views, and I was pretty positive about both confocal systems, and their support teams, over here in the UK.
Dr Keith J Morris Molecular Cytogenetics and Microscopy Core The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom
We know accurately only when we know little, with knowledge doubt increases.
- Johann Wolfgang von Goethe c1810
Although I also rather like his quotation:
We do not have to visit a madhouse to find disordered minds; our planet is the mental institution of the universe.
-----Original Message----- X-from: sekkio-at-mac.com [mailto:sekkio-at-mac.com] Sent: 13 December 2007 07:08 To: kjmorris-at-well.ox.ac.uk
HI Stephan, I think there is a little bit of confusion. The AOBS is a programmable beamsplitter (BS) and is, let me say, more evolute than the AOTF (about this I remember that it was introduced by Leica on 1993 approx... I had one Leica CLSM in 1987 and they told us about this new ugrade, well I am niot sure). More recently a AOBM - beam modulator has been introduced for delivering selected multiple wavelengths coming from a white light laser. This sound to be a sort of "best couple", AOBM+white light laser. AOBS has an important impact in wavelength selection in the detection scheme. It has to be carefully aligned and then it prevents from undesired reflections of the selected excitation wavelength a sort of paso doble, a gentleman and a lady dancing a Flamenco around an imaginary Bull like in a Corrida game... sorry for this... I think that the introduction of AOBS was a sort of puting brain instead of muscles in fluorescence microscopy. All my best Alby
Il giorno 12/dic/07, alle ore 11:30, nizets2-at-yahoo.com ha scritto:
} } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Good morning! } } The LSM510 serie introduced the AOTF, which allows the ultra-fast } switching between different incident wavelengthes. } This means that if you are scanning triple-stained samples, all 3 } wavelengthes are scanned sequentially (time-resolved). Well } sequential scanning is not a revolution, the key here is the ultra- } fast switching, which allows quasi-simultaneous ("real-time" for } your eyes) detection of different wavelengthes without the chance of } cross-talk. } Now I did some research on the net and I found that Leica developped } the same feature since 2002, although it is called AOBS ;-). I don't } know if both systems all fully comparable though. } } Best regards, } } Stephane, male (and a nice specimen, too ;-)) } } } } ----- Original Message ---- } X-from: "PhillipsT-at-missouri.edu" {PhillipsT-at-missouri.edu} } To: nizets2-at-yahoo.com } Sent: Monday, December 10, 2007 10:00:41 PM } Subject: [Microscopy] RE: LM - Confocal Opinions } } } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } I own a Zeiss 510 Meta NLO and am afraid I don't understand Stephane's } comment that the Zeiss separates the signal based on time. Perhaps } she } means that the system can, in real-time, separate out overlapping } emission signals using spectral imaging. If not, perhaps she could } expand on her comment. } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } -----Original Message----- } X-from: sekkio-at-mac.com [mailto:sekkio-at-mac.com] } Sent: Monday, December 10, 2007 2:28 PM } To: Phillips, Thomas E. } Subject: [Microscopy] LM - Confocal Opinions } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } About multiple fluorescence we got excellent results using a white } light laser coupled to the SP5AOBS } I think WLL is really a great advance in spectral imaging icluding } related applications } ciao } Alby } Il giorno 20/nov/07, alle ore 15:42, nizets2-at-yahoo.com ha scritto: } } } } } } } } } } ------------------------------------------------------------------------ } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------ } ---- } } } } Hi! } } } } I have used the previous Zeiss 510 microscope and liked it very } } much. It is user-friendly and powerful. } } I don't know the technical details of the Leica but in the Zeiss the } } different wavelengthes are separated not only by filters but also in } } time, so no read-through is possible. I must say this gives a } } welcome level of confidence and comfort. I did a lot of triple } } staining with great results. } } } } No interest blabla.... } } } } Regards, } } Stephane } } } } } } ----- Original Message ---- } } X-from: "christopher.hayden-at-novartis.com" } {christopher.hayden-at-novartis.com } } } } } To: nizets2-at-yahoo.com } } Sent: Monday, November 19, 2007 7:28:16 PM } } Subject: [Microscopy] LM - Confocal Opinions } } } } } } } } } } } ------------------------------------------------------------------------ } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------ } ---- } } } } Good Day, All: } } } } We're in the fortunate position of looking around at } } acquiring a } } confocal microscope sometime in 2008. Before we start hearing the } } pitches } } from the manufacturers, we'd like to hear from the "real" world. } } Specifically, what you have and what you think of it (ease-of-use, } } upgrades, level of control, service and support, etc.). } } After having a look around, it seems like the two contenders } } for } } us are the Zeiss 510 META NLO and the Leica TCS SP5. Flexibility is } } key } } for us, as it's going to have a fairly broad set of uses as both a } } research tool and as a development tool. } } Cost isn't critical at this point; we're basically being told } } to } } "get what we need". While we have people with confocal experience, } } each is } } } } a little biased that the system they know is the best one. } } } } Thanks much! } } -Chris } } } } } } } } ==============================Original } } Headers============================== of - Headers==============================
==============================Original Headers============================== 29, 20 -- From kjmorris-at-well.ox.ac.uk Thu Dec 13 09:54:02 2007 29, 20 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 29, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBDFs1fZ010828 29, 20 -- for {Microscopy-at-Microscopy.Com} ; Thu, 13 Dec 2007 09:54:02 -0600 29, 20 -- Received: from dhcp420.well.ox.ac.uk ([129.67.46.7] helo=cyto01) 29, 20 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 29, 20 -- id 1J2qNq-00064n-A7 29, 20 -- for Microscopy-at-Microscopy.Com; Thu, 13 Dec 2007 15:53:58 +0000 29, 20 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 29, 20 -- To: {Microscopy-at-Microscopy.Com} 29, 20 -- Subject: FW: [Microscopy] Re: LM - Confocal Opinions 29, 20 -- Date: Thu, 13 Dec 2007 15:56:24 -0000 29, 20 -- Message-ID: {000001c83da0$b66c6d40$072e4381-at-cyto01} 29, 20 -- MIME-Version: 1.0 29, 20 -- Content-Type: text/plain; 29, 20 -- charset="us-ascii" 29, 20 -- Content-Transfer-Encoding: 7bit 29, 20 -- X-Mailer: Microsoft Office Outlook 11 29, 20 -- Thread-Index: Acg9Vv9QQAHkURIqTgCOM3OkSv5i+AAEq8ggAAMHsDA= 29, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
==============================Original Headers============================== 39, 25 -- From greggps-at-umich.edu Thu Dec 13 15:53:47 2007 39, 25 -- Received: from OWA01.adsroot.itcs.umich.edu (owa01.adsroot.itcs.umich.edu [141.211.27.138]) 39, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBDLritq001074 39, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 13 Dec 2007 15:53:46 -0600 39, 25 -- Received: from ECLUST2-VS3.adsroot.itcs.umich.edu ([141.211.27.143]) by OWA01.adsroot.itcs.umich.edu with Microsoft SMTPSVC(6.0.3790.1830); 39, 25 -- Thu, 13 Dec 2007 16:53:42 -0500 39, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 39, 25 -- Content-class: urn:content-classes:message 39, 25 -- MIME-Version: 1.0 39, 25 -- Content-Type: text/plain; 39, 25 -- charset="US-ASCII" 39, 25 -- Subject: RE: [Microscopy] LM - Confocal Opinions- Zeiss and Leica should have similar capabilities 39, 25 -- Date: Thu, 13 Dec 2007 16:52:41 -0500 39, 25 -- Message-ID: {3F08051AB847224F9B89DAB301137EF402342D7E-at-ECLUST2-VS3.adsroot.itcs.umich.edu} 39, 25 -- In-Reply-To: {200712131603.lBDG34CD022492-at-ns.microscopy.com} 39, 25 -- X-MS-Has-Attach: 39, 25 -- X-MS-TNEF-Correlator: 39, 25 -- Thread-Topic: [Microscopy] LM - Confocal Opinions- Zeiss and Leica should have similar capabilities 39, 25 -- Thread-Index: Acg9oaacT1418rWhRC+rD01cnmxXZwAI4KEw 39, 25 -- References: {200712131603.lBDG34CD022492-at-ns.microscopy.com} 39, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} 39, 25 -- To: {Microscopy-at-Microscopy.Com} 39, 25 -- X-OriginalArrivalTime: 13 Dec 2007 21:53:42.0328 (UTC) FILETIME=[A0624380:01C83DD2] 39, 25 -- Content-Transfer-Encoding: 8bit 39, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lBDLritq001074 ==============================End of - Headers==============================
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Email: zhouda-at-umsl.edu Name: Dan Zhou
Title-Subject: [Filtered] Searching for an used Ultramicrotme for cutting TEM sections
Question: Dear All,
Our Electron Image and Spectroscopy Technique Lab is looking for an used ultramicrotome to cut TEM thin sections.
Please let me know If anyone is selling or giving away an used ultramicrotome.
Thank you very much for any leads,
Dan
Associate Research Professor and Laboratory Manager, Center for Nanoscience William L. Clay Building University of Missouri-St. Louis One University Boulevard St. Louis, Missouri 63121
To address Gregg's comment about proprietary formats.... As a long-time Zeiss user I am appreciative of the fact that Zeiss makes a reduced, free-ware version of the LSM software, called Image Browser, available as a download from their website. This allows viewing of the images as well as some minor adjustments AND export of the images into a variety of formats including 8, 12 or 16-bit Tifs.
Just a satisfied Zeiss customer... Lee
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-- Leona Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Weill Cornell Medical College
Be careful what you take on. I have just found that Leica no longer provides service or parts for the Ultracut E and other models. Check with the vendor to make sure what you are getting will be useful.
Also, I am curious if anyone knows of after-market service for the "abandoned" Ultracut models - like the Ultracut, and Ultracut E. Please share with the list, vendors welcome.
Thanks,
Dale Callaham Central Microscopy Facility University of Massachusetts at Amherst
zhouda-at-umsl.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both zhouda-at-umsl.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: zhouda-at-umsl.edu } Name: Dan Zhou } } Title-Subject: [Filtered] Searching for an used Ultramicrotme for cutting TEM sections } } Question: Dear All, } } Our Electron Image and Spectroscopy Technique Lab is looking for an used ultramicrotome to cut TEM thin sections. } } Please let me know If anyone is selling or giving away an used ultramicrotome. } } Thank you very much for any leads, } } Dan } } Associate Research Professor and Laboratory Manager, Center for Nanoscience William L. Clay Building University of Missouri-St. Louis One University Boulevard St. Louis, Missouri 63121 } } Phone: 314-516-4627 } Fax: 314-516-4628 } E-mail: zhouda-at-umsl.edu } } } Login Host: 134.124.125.224 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 12 -- From zaluzec-at-microscopy.com Thu Dec 13 21:20:16 2007 } 11, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBE3KDUL020336 } 11, 12 -- for {microscopy-at-microscopy.com} ; Thu, 13 Dec 2007 21:20:15 -0600 } 11, 12 -- Mime-Version: 1.0 } 11, 12 -- Message-Id: {p06240800c387a6dde61e-at-[206.69.208.22]} } 11, 12 -- Date: Thu, 13 Dec 2007 21:20:12 -0600 } 11, 12 -- To: microscopy-at-microscopy.com } 11, 12 -- From: zhouda-at-umsl.edu (by way of MicroscopyListserver) } 11, 12 -- Subject: viaWWW: Searching for an used Ultramicrotme for cutting TEM } 11, 12 -- sections } 11, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 22 -- From dac-at-research.umass.edu Fri Dec 14 14:41:12 2007 8, 22 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBEKfCLg009561 8, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Dec 2007 14:41:12 -0600 8, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 8, 22 -- (authenticated bits=0) 8, 22 -- by race3.oit.umass.edu (8.14.1/8.14.1) with ESMTP id lBEKfB54002100 8, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 8, 22 -- Fri, 14 Dec 2007 15:41:12 -0500 8, 22 -- Message-ID: {4762EAD3.5020209-at-research.umass.edu} 8, 22 -- Date: Fri, 14 Dec 2007 15:42:59 -0500 8, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 8, 22 -- Reply-To: dac-at-research.umass.edu 8, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.9) Gecko/20071030 SeaMonkey/1.1.6 8, 22 -- MIME-Version: 1.0 8, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} , zhouda-at-umsl.edu 8, 22 -- Subject: Re: Searching for used Ultramicrotme 8, 22 -- References: {200712140328.lBE3SNAh029305-at-ns.microscopy.com} 8, 22 -- In-Reply-To: {200712140328.lBE3SNAh029305-at-ns.microscopy.com} 8, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 22 -- Content-Transfer-Encoding: 7bit 8, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
-----Original Message----- X-from: Dale Callaham [mailto:dac-at-research.umass.edu] Sent: Friday, December 14, 2007 2:43 PM To: Microscopy Listserver; Zhou, Dan
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Email: qxing-at-ameslab.gov Name: Qingfeng Xing
Organization: Ames Lab
Title-Subject: [Filtered] Rhombic shape of TEM SAD spots
Question: The TEM SAD spots of an alloy show rhombic shape, rather than normal round shape. Could anyone give some comments or references on the origin of the shape? Due to the arrangement of electron or nuclei?
In some oxides such as PbO, the diffraction spots show strong streaks (looks somehow rhombic) at low temperatures because of the disorder of electrons (thank Prof Philippe Buffat for kind explanation). All the reports on charge ordering on my hands are on oxides. I have not found similar reports on alloys. Does anyone know the relating literature in alloys?
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Email: allison.van-de-meene-at-bbsrc.ac.uk Name: Allison van de Meene
Organization: Rothamsted Research, BBSRC Institute UK
Title-Subject: [Filtered] Cryo techniques course April 2008
Question: A course in sample preparation and cryo-microscopy techniques for both scanning and transmission electron microscopy will be held at Rothamsted Research in Harpenden, UK from 6-11th April 2008. Techniques covered will include the Tokuyasu method, rapid freezing of samples, freeze substitution, cryosectioning, thin film TEM, cryo-transfer and freeze fracture cryo-SEM. Attendees will have the opportunity to use state of the art equipment and instrumentation from a range of manufacturers. The format will include tutorials, demonstrations and hands-on practical sessions led by technical experts. Key speakers will supplement the techniques taught. Rothamsted Research has excellent air, rail and road links. Accommodation for 5 nights and all meals are included in the course fee. Please see the attached flyer and for further information please visit: www.rms.org.uk Or contact Victoria Lee Tel: +44 (0)1865 254769 Email: victoria-at-rms.org.uk Thank you,
Dr. Allison van de Meene Head of Bioimaging Centre for Bioimaging Plant Pathogen and Microbiology Department Rothamsted Research Harpenden Hertfordshire AL5 2JQ UK
=================================== Rothamsted Research is a company limited by guarantee, registered in England at the above address under the registration num
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Email: Yanling.Ge-at-tkk.fi Name: Yanling Ge
Organization: Helsinki University of Technology
Title-Subject: [Filtered] about commercial plasma cleaner
Question: Dear microscopist,
Our lab is going to buy a plasma cleaner for our Tecnai F20 TEM. The price about several commercial product, SPI, SBT, Fischione, Gatan, can be from 12K to over 50K. Is the technology is really so superior in these high price product? Could someone give some guidance for selection of plasma cleaner?
Such cautions are useful, but many of us can tell you that there's not much like the original Ultracut microtomes for durability. You really have to abuse them to need anything more than a belt replacement and an occasional cleaning and regreasing. If you have just a little mechanical inclination, or know someone who does, I'd recommend most Ultracuts for your lab.
I just opened up an early model Ultracut that looks like it has been dropped off a table, amongst other abuses. I opened it up to find the internals in excellent condition (after the plastic was swept aside) and only a belt needs replacing.
Regards, ~Gregg (Who does not have any commercial affiliations to this product, but has experience with multiple A/O Ultracuts and Reichiert Ultracut Es.)
Gregg Sobocinski Imaging Specialist/Microscopist Univeristy of Michigan Ann Arbor, Michigan USA
-----Original Message----- X-from: dac-at-research.umass.edu [mailto:dac-at-research.umass.edu] Sent: Friday, December 14, 2007 3:50 PM To: Sobocinski, Gregg
Hi Dan,
Be careful what you take on. I have just found that Leica no longer provides service or parts for the Ultracut E and other models. Check with the vendor to make sure what you are getting will be useful.
Also, I am curious if anyone knows of after-market service for the "abandoned" Ultracut models - like the Ultracut, and Ultracut E. Please share with the list, vendors welcome.
Thanks,
Dale Callaham Central Microscopy Facility University of Massachusetts at Amherst
zhouda-at-umsl.edu wrote: } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } ------------------------------------------------------------------------ --- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both zhouda-at-umsl.edu as well as the MIcroscopy Listserver } ------------------------------------------------------------------------ --- } } Email: zhouda-at-umsl.edu } Name: Dan Zhou } } Title-Subject: [Filtered] Searching for an used Ultramicrotme for cutting TEM sections } } Question: Dear All, } } Our Electron Image and Spectroscopy Technique Lab is looking for an used ultramicrotome to cut TEM thin sections. } } Please let me know If anyone is selling or giving away an used ultramicrotome. } } Thank you very much for any leads, } } Dan } } Associate Research Professor and Laboratory Manager, Center for Nanoscience William L. Clay Building University of Missouri-St. Louis One University Boulevard St. Louis, Missouri 63121 } } Phone: 314-516-4627 } Fax: 314-516-4628 } E-mail: zhouda-at-umsl.edu } } } Login Host: 134.124.125.224 } ------------------------------------------------------------------------ --- } } ==============================Original Headers============================== } 11, 12 -- From zaluzec-at-microscopy.com Thu Dec 13 21:20:16 2007 } 11, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBE3KDUL020336 } 11, 12 -- for {microscopy-at-microscopy.com} ; Thu, 13 Dec 2007 21:20:15 -0600 } 11, 12 -- Mime-Version: 1.0 } 11, 12 -- Message-Id: {p06240800c387a6dde61e-at-[206.69.208.22]} } 11, 12 -- Date: Thu, 13 Dec 2007 21:20:12 -0600 } 11, 12 -- To: microscopy-at-microscopy.com } 11, 12 -- From: zhouda-at-umsl.edu (by way of MicroscopyListserver) } 11, 12 -- Subject: viaWWW: Searching for an used Ultramicrotme for cutting TEM } 11, 12 -- sections } 11, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 22 -- From dac-at-research.umass.edu Fri Dec 14 14:41:12 2007 8, 22 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBEKfCLg009561 8, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Dec 2007 14:41:12 -0600 8, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 8, 22 -- (authenticated bits=0) 8, 22 -- by race3.oit.umass.edu (8.14.1/8.14.1) with ESMTP id lBEKfB54002100 8, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 8, 22 -- Fri, 14 Dec 2007 15:41:12 -0500 8, 22 -- Message-ID: {4762EAD3.5020209-at-research.umass.edu} 8, 22 -- Date: Fri, 14 Dec 2007 15:42:59 -0500 8, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 8, 22 -- Reply-To: dac-at-research.umass.edu 8, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.9) Gecko/20071030 SeaMonkey/1.1.6 8, 22 -- MIME-Version: 1.0 8, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} , zhouda-at-umsl.edu 8, 22 -- Subject: Re: Searching for used Ultramicrotme 8, 22 -- References: {200712140328.lBE3SNAh029305-at-ns.microscopy.com} 8, 22 -- In-Reply-To: {200712140328.lBE3SNAh029305-at-ns.microscopy.com} 8, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 22 -- Content-Transfer-Encoding: 7bit 8, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
==============================Original Headers============================== 16, 25 -- From greggps-at-umich.edu Mon Dec 17 08:23:14 2007 16, 25 -- Received: from OWA02.adsroot.itcs.umich.edu (owa02.adsroot.itcs.umich.edu [141.211.27.139]) 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBHEND0h011456 16, 25 -- for {microscopy-at-microscopy.com} ; Mon, 17 Dec 2007 08:23:13 -0600 16, 25 -- Received: from ECLUST2-VS3.adsroot.itcs.umich.edu ([141.211.27.143]) by OWA02.adsroot.itcs.umich.edu with Microsoft SMTPSVC(6.0.3790.1830); 16, 25 -- Mon, 17 Dec 2007 09:23:13 -0500 16, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 25 -- Content-class: urn:content-classes:message 16, 25 -- MIME-Version: 1.0 16, 25 -- Content-Type: text/plain; 16, 25 -- charset="us-ascii" 16, 25 -- Subject: RE: [Microscopy] Searching for used Ultramicrotome-Cautions about Ultracut service 16, 25 -- Date: Mon, 17 Dec 2007 09:23:11 -0500 16, 25 -- Message-ID: {3F08051AB847224F9B89DAB301137EF402342FB8-at-ECLUST2-VS3.adsroot.itcs.umich.edu} 16, 25 -- In-Reply-To: {200712142050.lBEKoFSK021171-at-ns.microscopy.com} 16, 25 -- X-MS-Has-Attach: 16, 25 -- X-MS-TNEF-Correlator: 16, 25 -- Thread-Topic: [Microscopy] Searching for used Ultramicrotome-Cautions about Ultracut service 16, 25 -- Thread-Index: Acg+ku8jpa5iXyXYTbukA4RupxDDygCI7vdA 16, 25 -- References: {200712142050.lBEKoFSK021171-at-ns.microscopy.com} 16, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} 16, 25 -- To: {microscopy-at-microscopy.com} 16, 25 -- X-OriginalArrivalTime: 17 Dec 2007 14:23:13.0097 (UTC) FILETIME=[5B5BEB90:01C840B8] 16, 25 -- Content-Transfer-Encoding: 8bit 16, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lBHEND0h011456 ==============================End of - Headers==============================
The technology is actually fairly simple. The problem is that it must be built, and someone takes responsibility for the line voltage and HV potential hazards, etc., etc. I have, use, and love our Harrick plasma cleaner. It is wonderful and easy to use. I saw someone mention a price on the list recently for the Harrick at ~$2500USD - I don't know if that is accurate. Buy what you can, and build what you MUST.
IF you are using it to just "glow discharge" grids to make carbon wettable you don't really need the high cleaning power levels of some of the expensive units - I always operate the Harrick at the low power setting for 10 - 30s.
Simply, a plasma is a plasma for the purpose at hand; it is the same as a glow discharge. Residual air (at ~ 0.1 torr) is ripped apart by the energy source and the activated molecules do the work. There are different ways of generating a plasma: High Voltage AC or DC, capacitively coupled RF, and inductively coupled RF (the Harrick does this). The Harrick is simply an oscillator with the chamber sitting inside the inductor; the RF field down the center of the solenoid (at ~ 13.56MHz, I think) provides the energy. It is an extremely simple vacuum tube circuit - the tubes cost about $8 (usd) each. Again, the Harrick unit is well built and works well straight out of the box; we have had ours for years.
I can send some information I have collected on glow-discharge and plasmas if you are interested.
Cheers,
Dale Callaham The University of Massachusetts at Amherst
Yanling.Ge-at-tkk.fi wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both Yanling.Ge-at-tkk.fi as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: Yanling.Ge-at-tkk.fi } Name: Yanling Ge } } Organization: Helsinki University of Technology } } Title-Subject: [Filtered] about commercial plasma cleaner } } Question: Dear microscopist, } } Our lab is going to buy a plasma cleaner for our Tecnai F20 TEM. The price about several commercial product, SPI, SBT, Fischione, Gatan, can be from 12K to over 50K. Is the technology is really so superior in these high price product? Could someone give some guidance for selection of plasma cleaner? } } Thannks in advance! } } Yanling Ge } } Login Host: 130.233.105.251 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Mon Dec 17 07:44:52 2007 } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBHDipts007619 } 9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 17 Dec 2007 07:44:51 -0600 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240802c38c2dc558b1-at-[206.69.208.22]} } 9, 11 -- Date: Mon, 17 Dec 2007 07:44:50 -0600 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: Yanling.Ge-at-tkk.fi (by way of MicroscopyListserver) } 9, 11 -- Subject: viaWWW: about commercial plasma cleaner } 9, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 22 -- From dac-at-research.umass.edu Mon Dec 17 09:04:58 2007 8, 22 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBHF4vPM024825 8, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Dec 2007 09:04:58 -0600 8, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 8, 22 -- (authenticated bits=0) 8, 22 -- by race2.oit.umass.edu (8.14.1/8.14.1) with ESMTP id lBHF4ult003142 8, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 8, 22 -- Mon, 17 Dec 2007 10:04:57 -0500 8, 22 -- Message-ID: {47669089.5000501-at-research.umass.edu} 8, 22 -- Date: Mon, 17 Dec 2007 10:06:49 -0500 8, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 8, 22 -- Reply-To: dac-at-research.umass.edu 8, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.9) Gecko/20071030 SeaMonkey/1.1.6 8, 22 -- MIME-Version: 1.0 8, 22 -- To: Yanling.Ge-at-tkk.fi, Microscopy Listserver {Microscopy-at-microscopy.com} 8, 22 -- Subject: Re: [Microscopy] viaWWW: about commercial plasma cleaner 8, 22 -- References: {200712171355.lBHDtSnK032458-at-ns.microscopy.com} 8, 22 -- In-Reply-To: {200712171355.lBHDtSnK032458-at-ns.microscopy.com} 8, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 22 -- Content-Transfer-Encoding: 7bit 8, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
I agree with this advice fully. The key is "original Ultracut". The Ultracuts got progressively more complicated over time. The Ultracut (no letter) is a very basic design that would be easy to maintain; it is an analog servo-motor speed control, and fully mechanical (no steppers, etc) advance; an advanced user or shop could maintain this. The "E" got more "digital" and while they did supply schematics, the motors and logic will be more difficult for a user to deal with. The "S" is more advanced electronically, uses a microcontroller, and has advanced features like an optical encoder plate for the cutting zone determination, and this line would be more difficult to maintain without schematics, and the special parts would be difficult to obtain if Leica ever drops support for it.
So yes, the "original" Ultracut is the one for the ages....
And I would like to thank all who posted the information about MOC (Microscopical Optical Consulting Inc.) in Valley Cottage, NY, for aftermarket microtome service! Thank You!
MOC Helmut Patzig Ph: 845-268-6450 Fax: 845-268-0172
Dale
greggps-at-umich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Such cautions are useful, but many of us can tell you that there's not } much like the original Ultracut microtomes for durability. You really } have to abuse them to need anything more than a belt replacement and an } occasional cleaning and regreasing. If you have just a little mechanical } inclination, or know someone who does, I'd recommend most Ultracuts for } your lab. } } I just opened up an early model Ultracut that looks like it has been } dropped off a table, amongst other abuses. I opened it up to find the } internals in excellent condition (after the plastic was swept aside) and } only a belt needs replacing. } } Regards, } ~Gregg (Who does not have any commercial affiliations to this product, } but has experience with multiple A/O Ultracuts and Reichiert Ultracut } Es.) } } Gregg Sobocinski } Imaging Specialist/Microscopist } Univeristy of Michigan } Ann Arbor, Michigan } USA } } -----Original Message----- } X-from: dac-at-research.umass.edu [mailto:dac-at-research.umass.edu] } Sent: Friday, December 14, 2007 3:50 PM } To: Sobocinski, Gregg } Subject: [Microscopy] Re: Searching for used Ultramicrotme } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } Hi Dan, } } Be careful what you take on. I have just found that Leica no longer } provides service or parts for the Ultracut E and other models. Check } with the vendor to make sure what you are getting will be useful. } } Also, I am curious if anyone knows of after-market service for the } "abandoned" Ultracut models - like the Ultracut, and Ultracut E. Please } share with the list, vendors welcome. } } Thanks, } } Dale Callaham } Central Microscopy Facility } University of Massachusetts at Amherst } } } } zhouda-at-umsl.edu wrote: } ------------------------------------------------------------------------ } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------ } ---- } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } ------------------------------------------------------------------------ } --- } } Remember this posting is most likely not from a Subscriber, so when } replying } } please copy both zhouda-at-umsl.edu as well as the MIcroscopy } Listserver } ------------------------------------------------------------------------ } --- } } Email: zhouda-at-umsl.edu } } Name: Dan Zhou } } } } Title-Subject: [Filtered] Searching for an used Ultramicrotme for } cutting TEM sections } } Question: Dear All, } } } } Our Electron Image and Spectroscopy Technique Lab is looking for an } used ultramicrotome to cut TEM thin sections. } } Please let me know If anyone is selling or giving away an used } ultramicrotome. } } Thank you very much for any leads, } } } } Dan } } } } Associate Research Professor and Laboratory Manager, Center for } Nanoscience William L. Clay Building University of Missouri-St. Louis } One University Boulevard St. Louis, Missouri 63121 } } } } Phone: 314-516-4627 } } Fax: 314-516-4628 } } E-mail: zhouda-at-umsl.edu } } } } } } Login Host: 134.124.125.224 } } } ------------------------------------------------------------------------ } --- } } } } ==============================Original } Headers============================== } } 11, 12 -- From zaluzec-at-microscopy.com Thu Dec 13 21:20:16 2007 } } 11, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } } 11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id lBE3KDUL020336 } } 11, 12 -- for {microscopy-at-microscopy.com} ; Thu, 13 Dec 2007 } 21:20:15 -0600 } } 11, 12 -- Mime-Version: 1.0 } } 11, 12 -- Message-Id: {p06240800c387a6dde61e-at-[206.69.208.22]} } } 11, 12 -- Date: Thu, 13 Dec 2007 21:20:12 -0600 } } 11, 12 -- To: microscopy-at-microscopy.com } } 11, 12 -- From: zhouda-at-umsl.edu (by way of MicroscopyListserver) } } 11, 12 -- Subject: viaWWW: Searching for an used Ultramicrotme for } cutting TEM } } 11, 12 -- sections } } 11, 12 -- Content-Type: text/plain; charset="us-ascii" } } ==============================End of - } Headers============================== } } ==============================Original } Headers============================== } 8, 22 -- From dac-at-research.umass.edu Fri Dec 14 14:41:12 2007 } 8, 22 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu } [128.119.101.39]) } 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id lBEKfCLg009561 } 8, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Dec 2007 } 14:41:12 -0600 } 8, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu } [128.119.55.30]) } 8, 22 -- (authenticated bits=0) } 8, 22 -- by race3.oit.umass.edu (8.14.1/8.14.1) with ESMTP id } lBEKfB54002100 } 8, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 } verify=NOT); } 8, 22 -- Fri, 14 Dec 2007 15:41:12 -0500 } 8, 22 -- Message-ID: {4762EAD3.5020209-at-research.umass.edu} } 8, 22 -- Date: Fri, 14 Dec 2007 15:42:59 -0500 } 8, 22 -- From: Dale Callaham {dac-at-research.umass.edu} } 8, 22 -- Reply-To: dac-at-research.umass.edu } 8, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; } rv:1.8.1.9) Gecko/20071030 SeaMonkey/1.1.6 } 8, 22 -- MIME-Version: 1.0 } 8, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} , } zhouda-at-umsl.edu } 8, 22 -- Subject: Re: Searching for used Ultramicrotme } 8, 22 -- References: {200712140328.lBE3SNAh029305-at-ns.microscopy.com} } 8, 22 -- In-Reply-To: {200712140328.lBE3SNAh029305-at-ns.microscopy.com} } 8, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 22 -- Content-Transfer-Encoding: 7bit } 8, 22 -- X-Whitelist: TRUE } ==============================End of - } Headers============================== } } } ==============================Original Headers============================== } 16, 25 -- From greggps-at-umich.edu Mon Dec 17 08:23:14 2007 } 16, 25 -- Received: from OWA02.adsroot.itcs.umich.edu (owa02.adsroot.itcs.umich.edu [141.211.27.139]) } 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBHEND0h011456 } 16, 25 -- for {microscopy-at-microscopy.com} ; Mon, 17 Dec 2007 08:23:13 -0600 } 16, 25 -- Received: from ECLUST2-VS3.adsroot.itcs.umich.edu ([141.211.27.143]) by OWA02.adsroot.itcs.umich.edu with Microsoft SMTPSVC(6.0.3790.1830); } 16, 25 -- Mon, 17 Dec 2007 09:23:13 -0500 } 16, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 16, 25 -- Content-class: urn:content-classes:message } 16, 25 -- MIME-Version: 1.0 } 16, 25 -- Content-Type: text/plain; } 16, 25 -- charset="us-ascii" } 16, 25 -- Subject: RE: [Microscopy] Searching for used Ultramicrotome-Cautions about Ultracut service } 16, 25 -- Date: Mon, 17 Dec 2007 09:23:11 -0500 } 16, 25 -- Message-ID: {3F08051AB847224F9B89DAB301137EF402342FB8-at-ECLUST2-VS3.adsroot.itcs.umich.edu} } 16, 25 -- In-Reply-To: {200712142050.lBEKoFSK021171-at-ns.microscopy.com} } 16, 25 -- X-MS-Has-Attach: } 16, 25 -- X-MS-TNEF-Correlator: } 16, 25 -- Thread-Topic: [Microscopy] Searching for used Ultramicrotome-Cautions about Ultracut service } 16, 25 -- Thread-Index: Acg+ku8jpa5iXyXYTbukA4RupxDDygCI7vdA } 16, 25 -- References: {200712142050.lBEKoFSK021171-at-ns.microscopy.com} } 16, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} } 16, 25 -- To: {microscopy-at-microscopy.com} } 16, 25 -- X-OriginalArrivalTime: 17 Dec 2007 14:23:13.0097 (UTC) FILETIME=[5B5BEB90:01C840B8] } 16, 25 -- Content-Transfer-Encoding: 8bit } 16, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lBHEND0h011456 } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 23 -- From dac-at-research.umass.edu Mon Dec 17 09:49:29 2007 6, 23 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBHFnTB6005936 6, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Dec 2007 09:49:29 -0600 6, 23 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 6, 23 -- (authenticated bits=0) 6, 23 -- by race5.oit.umass.edu (8.14.1/8.14.1) with ESMTP id lBHFnSOF001716 6, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 6, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Dec 2007 10:49:29 -0500 6, 23 -- Message-ID: {47669AF8.8060409-at-research.umass.edu} 6, 23 -- Date: Mon, 17 Dec 2007 10:51:20 -0500 6, 23 -- From: Dale Callaham {dac-at-research.umass.edu} 6, 23 -- Reply-To: dac-at-research.umass.edu 6, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.9) Gecko/20071030 SeaMonkey/1.1.6 6, 23 -- MIME-Version: 1.0 6, 23 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 23 -- Subject: Re: [Microscopy] RE: Searching for used Ultramicrotome-Cautions about 6, 23 -- Ultracut service 6, 23 -- References: {200712171427.lBHERYd9020415-at-ns.microscopy.com} 6, 23 -- In-Reply-To: {200712171427.lBHERYd9020415-at-ns.microscopy.com} 6, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 23 -- Content-Transfer-Encoding: 7bit 6, 23 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Does anyone know of any other good etchant(s) other than Keller's and Barker's reagents for 6xxx aluminum alloys? Our interest is in grain size measurement.
Also, does any one know any outlet that sells ready-made Keller's etchant? We do not want to mix the chemicals by ourselves due to some local restrictions.
Please, contact me privately if you market Keller's etchant.
Thanks for your anticipated assistance.
Ike Oguocha
Department of Mechanical Engineering 57 Campus Drive University of Saskatchewan Saskatoon, SK, S7N 5A9, Canada
Phone: (306)966-7832 Fax: (306)966-5427
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==============================Original Headers============================== 11, 20 -- From oguocha-at-yahoo.com Tue Dec 18 18:50:36 2007 11, 20 -- Received: from web53702.mail.re2.yahoo.com (web53702.mail.re2.yahoo.com [206.190.37.23]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lBJ0oaY1020348 11, 20 -- for {microscopy-at-microscopy.com} ; Tue, 18 Dec 2007 18:50:36 -0600 11, 20 -- Received: (qmail 35692 invoked by uid 60001); 19 Dec 2007 00:50:35 -0000 11, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 20 -- s=s1024; d=yahoo.com; 11, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 11, 20 -- b=D5ktzjWoEUrVO5dLFzUcBzvVxCX4DnbVldXBUlT31kTGGxMnFeAn1UILn4snh5GjnTWKiZZHYXctNBm3jsfc/csFO6kbovXpaIMMJBcGG2BN9FJo+dTk1ILtFSXGUrxrnaIrt5YvfnFK9SwunFp6z5ZhoufsrHdjArg4ML0i0Sg=; 11, 20 -- X-YMail-OSG: kCQIUugVM1lLn4FU026QbtKnCo.I4XDR7YFZIazlQBadyh2awzaE_hk8nnkQF5N4lx0HA7d8cYVKgvAY7PZ_QagttcXPyzsgVuAw4vT8.jwK5_NOixayLPdwK8iQwhAJBvl_GSBWhZ2YLmmi7iBs3RqBxQ-- 11, 20 -- Received: from [128.233.23.104] by web53702.mail.re2.yahoo.com via HTTP; Tue, 18 Dec 2007 16:50:35 PST 11, 20 -- Date: Tue, 18 Dec 2007 16:50:35 -0800 (PST) 11, 20 -- From: Ike Oguocha {oguocha-at-yahoo.com} 11, 20 -- Subject: Etchants for 6XXX aluminum alloys 11, 20 -- To: microscopy-at-microscopy.com 11, 20 -- In-Reply-To: {200712052300.lB5N0JMI025069-at-ns.microscopy.com} 11, 20 -- MIME-Version: 1.0 11, 20 -- Content-Type: text/plain; charset=iso-8859-1 11, 20 -- Content-Transfer-Encoding: 8bit 11, 20 -- Message-ID: {556998.33192.qm-at-web53702.mail.re2.yahoo.com} ==============================End of - Headers==============================
If you have EBSD, a polished sample should produce nice grain info. Etching enhances FSD images. I do many Al films and solids without any etching other than DI water.
gary g.
At 04:53 PM 12/18/2007, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 5, 20 -- From gary-at-gaugler.com Tue Dec 18 19:35:02 2007 5, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id lBJ1Z2YR001199 5, 20 -- for {microscopy-at-microscopy.com} ; Tue, 18 Dec 2007 19:35:02 -0600 5, 20 -- Message-Id: {200712190135.lBJ1Z2YR001199-at-ns.microscopy.com} 5, 20 -- Received: (qmail 4393 invoked from network); 18 Dec 2007 17:35:01 -0800 5, 20 -- Received: by simscan 1.1.0 ppid: 4388, pid: 4391, t: 0.1072s 5, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 5, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 5, 20 -- by qsmtp3 with SMTP; 18 Dec 2007 17:35:01 -0800 5, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 5, 20 -- Date: Tue, 18 Dec 2007 17:35:01 -0800 5, 20 -- To: oguocha-at-yahoo.com 5, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 5, 20 -- Subject: Re: [Microscopy] Etchants for 6XXX aluminum alloys 5, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 5, 20 -- In-Reply-To: {200712190053.lBJ0rsZu023036-at-ns.microscopy.com} 5, 20 -- References: {200712190053.lBJ0rsZu023036-at-ns.microscopy.com} 5, 20 -- Mime-Version: 1.0 5, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-49FE222B ==============================End of - Headers==============================
I have a couple of general questions about the use of EELS in certain experimental situations. I would appreciate comments on the feasibility of identifying elements using this technique in the following scenarios:
In certain situations, cells can be influenced so that there is a rapid influx of Ca. This can be seen and documented using confocal microscopy. Would it be possible to capture the Ca++ distribution in the cells with greater resolution if cells were treated to induce the Ca++ influx and then immediately subjected to high pressure freezing? They would be FS and resin embedded prior to TEM analysis with EELS.
There is a theory that, also under certain conditions, zinc will enter the cells via Ca++ channels. Would it be possible to pick up the Zn++ using EELS?
Is it possible to simultaneously do a standard tomogram and also an EELS tomogram so that the two could be superimposed?
Any references documenting transient ion flux using EELS would be greatly appreciated as would any comments from those experienced using the technique.
Thanks, Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 11, 21 -- From dsherman-at-purdue.edu Tue Dec 18 20:12:27 2007 11, 21 -- Received: from exchange.purdue.edu (1061exfe01.adpc.purdue.edu [128.210.63.221]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJ2CQ2F014359 11, 21 -- for {microscopy-at-microscopy.com} ; Tue, 18 Dec 2007 20:12:26 -0600 11, 21 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by exchange.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 11, 21 -- Tue, 18 Dec 2007 21:12:26 -0500 11, 21 -- Received: from 74.140.109.252 ([74.140.109.252]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 11, 21 -- Wed, 19 Dec 2007 02:12:26 +0000 11, 21 -- User-Agent: Microsoft-Entourage/11.3.6.070618 11, 21 -- Date: Tue, 18 Dec 2007 21:12:19 -0500 11, 21 -- Subject: EELS information 11, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 11, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 11, 21 -- Message-ID: {C38DE833.15856%dsherman-at-purdue.edu} 11, 21 -- Thread-Topic: EELS information 11, 21 -- Thread-Index: AchB5JUb02zwcK3XEdytOAAbY5Fdpg== 11, 21 -- Mime-version: 1.0 11, 21 -- Content-type: text/plain; 11, 21 -- charset="US-ASCII" 11, 21 -- Content-transfer-encoding: 7bit 11, 21 -- X-OriginalArrivalTime: 19 Dec 2007 02:12:26.0704 (UTC) FILETIME=[99B2CD00:01C841E4] ==============================End of - Headers==============================
In case some of you believe all carbon substrates are manufactured to be free of x-ray spectral peaks, I just discovered small nickel wire in a type of "ultra-smooth" double-sticky carbon dots. My impression is that it is needed to make the surface smooth. I won't reveal the manufacturer here, and I'll admit they work relatively well for background-free imaging, but if users require their carbon substrates to be contaminant-free they should check their stock or ask before purchasing.
holidays' cheerios :o) michael shaffer Memorial University St. John's Newfoundland
==============================Original Headers============================== 2, 26 -- From michael-at-shaffer.net Wed Dec 19 05:02:49 2007 2, 26 -- Received: from smtprelay.hostedemail.com (smtprelay0115.hostedemail.com [216.40.44.115]) 2, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJB2ne3008477 2, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 05:02:49 -0600 2, 26 -- Received: from emd2-omf02.hostedemail.com (ff-bigip1 [10.5.19.254]) 2, 26 -- by smtprelay03.hostedemail.com (Postfix) with ESMTP id 075F4485A8 2, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 11:02:49 +0000 (UTC) 2, 26 -- X-SpamScore: 2 2, 26 -- X-Spamcatcher-Summary: 2,0,0,a4f184466f1c2f7a,66de95b916e524ad,michael-at-shaffer.net,-,RULES_HIT:10:355:379:472:539:541:542:966:988:989:1155:1156:1160:1260:1277:1311:1313:1314:1345:1437:1515:1516:1518:1534:1538:1593:1594:1711:1714:1730:1747:1766:1792:2075:2078:2196:2199:2378:2393:2559:2562:2691:3350:3636:3865:3866:3867:3868:3869:3870:3871:3872:3873:3874:4385:5007:6261,0,RBL:none,CacheIP:none,Bayesian:0.5,0.5,0.5,Netcheck:none,DomainCache:0,MSF:not bulk,SPF:,MSBL:none,DNSBL:none 2, 26 -- X-Spamcatcher-Explanation: 2, 26 -- Received: from rarewolf (CPE00c0f0237e36-CM0018682b4b4c.cpe.net.cable.rogers.com [72.139.87.170]) 2, 26 -- (Authenticated sender: michael-at-shaffer.net) 2, 26 -- by emd2-omf02.hostedemail.com (Postfix) with ESMTP 2, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 11:02:48 +0000 (UTC) 2, 26 -- From: "michael shaffer" {michael-at-shaffer.net} 2, 26 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com} 2, 26 -- Subject: Ni in carbon dots 2, 26 -- Date: Wed, 19 Dec 2007 07:32:43 -0330 2, 26 -- Message-ID: {002f01c8422e$afadc930$4701a8c0-at-rarewolf} 2, 26 -- MIME-Version: 1.0 2, 26 -- Content-Type: text/plain; 2, 26 -- charset="us-ascii" 2, 26 -- Content-Transfer-Encoding: 7bit 2, 26 -- X-Mailer: Microsoft Office Outlook 11 2, 26 -- Thread-Index: AchCLAuoIb49sPf2QHC44yU/bnG/aw== 2, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
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Email: andreana782-at-yahoo.com.tw Name: Jean Chen
Title-Subject: [Filtered] elemental composition
Question: I was wondering if anyone can give a comment on which analytical tool can give the best composition ratio of the solid materials. As far as I know, RBS can determine the composition of bulk sample. XPS can determine the composition of the surface. Does anyone know which one can give a more acurate composition? By the way, RBS should be a non-destructive tool or a destructive tool?
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This can be an important issue. Is the wire magnetic? Or is it a non-magnetic alloy? Try putting the dots next to a strong magnet and see if they stick. I would be concerned if the wire could be magnetized and then adversely affect high res imaging as well as elemental microanalysis.
Can I suggest that you contact the vendor and find out if this was a one time event (i.e. the wrong wire was used in a batch) or if this is standard procedure.
If it is standard, it would be appropriate for the vendor to comment on this of his/her own accord on this forum.
Nestor Your Friendly Neighborhood SysOp
} Date: Wed, 19 Dec 2007 05:02:50 -0600 } To: zaluzec-at-microscopy.com } From: michael-at-shaffer.net } Reply-to: michael-at-shaffer.net } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] Ni in carbon dots } X-lewp: MicroscopyListSpam NAGS } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 10, 11 -- From zaluzec-at-microscopy.com Wed Dec 19 08:11:37 2007 10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJEBahM016024 10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 08:11:37 -0600 10, 11 -- Mime-Version: 1.0 10, 11 -- Message-Id: {p06240803c38ed3ea4c5f-at-[206.69.208.22]} 10, 11 -- Date: Wed, 19 Dec 2007 08:11:35 -0600 10, 11 -- To: microscopy-at-microscopy.com 10, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 10, 11 -- Subject: Re: Ni in carbon dots 10, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
In principle RBS is non-destructive. But it really depends on: 1) what kind of beam is used and what current 2) what type of specimen is analyzed It might happen that fragile specimens would be destroyed by ion beam of typically used alpha particle beam. On the other hand, proton beam of low current can be used to analyse some very fragile specimens which can be destroyed even when pumping experimental chamber before measurements. Careful pumping and careful measurements would leave such specimens intact - i.e. the technique is non-destructive in such cases.
RBS can certainly give accurate composition of major elements with ca. 5% error or similar.
I do not have experience with XPS.
What kind of specimens do you have?
Regards
Wojciech Przybylowicz
----- Original Message ----- X-from: {andreana782-at-yahoo.com.tw} To: {przybylowicz-at-tlabs.ac.za} Sent: Wednesday, December 19, 2007 3:54 PM
Dear Yanling,
While it is reasonably straightforward to generate a plasma, there are important factors that can affect TEM samples.
The first is gas chemistry. A plasma creates a wide variety of ions and radicals. Most of the important chemistry is the result of radicals. While oxygen radicals are the most important species involved in removing hydrocarbons from sample surfaces, other species and ions can be significant. Many vendors recommend high purity gases rather than air so that the gas chemistry can be controlled. A common gas chemistry is Ar-O2. Argon is not reactive and can affect the sample only by ballistic impact. In a poorly tuned or designed system positively charged Ar ions can damage thin specimens and sometimes sputter material from holders. Replacing Ar with H2, for instance, results in much lighter positive ions that do not damage specimens or holders. In addition, the design of the system affects how much energy the ions have, and thus how likely they are to damage your specimen.
The second major factor is tool automation. More automated systems, if well designed, can result in more consistent results. For instance, the output impedance of the RF power source should be matched to the impedance of the chamber/plasma. Some systems have an auto-matching network that insures that power is delivered efficiently to the plasma, rather than being reflected back due to an impedance mismatch. Systems that rely on manually matching the impedance are prone to user errors that can dramatically affect cleaning efficiency. Likewise, if the gas flow is set manually, there is a danger that untrained users can get poor results. For example, if the gas flow (thus pressure) is too low then the mean free path of positive ions is increased. This may result in increased sputtering of the specimen.
I think a sophisticated user can get good results from an inexpensive system. What you should get from a more expensive system is more flexibility and more consistent results. TEM specimens can be quite precious. Every facility has to weigh the costs/benefits against the risk of damaging specimens.
Best regards, Steve Coyle
Disclaimer: Gatan manufactures a high-end plasma cleaner, which we developed because of the reasons mentioned above.
========================================= Steven T. Coyle, Ph.D. Gatan Inc. 5794 W. Las Positas Blvd. Pleasanton, CA 94588 Direct (925) 224 7383 Fax (925) 463 0204 E-mail scoyle-at-gatan.com =========================================
Yanling.Ge-at-tkk.fi wrote: } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } This Question/Comment was submitted to the Microscopy Listserver using } the WWW based Form at http://www.microscopy.com/MLFormMail.html } ---------------------------------------------------------------------- } ----- Remember this posting is most likely not from a Subscriber, so } when replying } please copy both Yanling.Ge-at-tkk.fi as well as the MIcroscopy Listserver } ---------------------------------------------------------------------- } ----- } } Email: Yanling.Ge-at-tkk.fi } Name: Yanling Ge } } Organization: Helsinki University of Technology } } Title-Subject: [Filtered] about commercial plasma cleaner } } Question: Dear microscopist, } } Our lab is going to buy a plasma cleaner for our Tecnai F20 TEM. The price about several commercial product, SPI, SBT, Fischione, Gatan, can be from 12K to over 50K. Is the technology is really so superior in these high price product? Could someone give some guidance for selection of plasma cleaner? } } Thannks in advance! } } Yanling Ge } } Login Host: 130.233.105.251 } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Mon Dec 17 07:44:52 2007 9, 11 -- } Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBHDipts007619 } 9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 17 Dec 2007 07:44:51 -0600 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240802c38c2dc558b1-at-[206.69.208.22]} } 9, 11 -- Date: Mon, 17 Dec 2007 07:44:50 -0600 9, 11 -- To: } microscopy-at-microscopy.com 9, 11 -- From: Yanling.Ge-at-tkk.fi (by way of } MicroscopyListserver) 9, 11 -- Subject: viaWWW: about commercial } plasma cleaner 9, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 22 -- From dac-at-research.umass.edu Mon Dec 17 09:04:58 2007 8, 22 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBHF4vPM024825 8, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Dec 2007 09:04:58 -0600 8, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 8, 22 -- (authenticated bits=0) 8, 22 -- by race2.oit.umass.edu (8.14.1/8.14.1) with ESMTP id lBHF4ult003142 8, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 8, 22 -- Mon, 17 Dec 2007 10:04:57 -0500 8, 22 -- Message-ID: {47669089.5000501-at-research.umass.edu} 8, 22 -- Date: Mon, 17 Dec 2007 10:06:49 -0500 8, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 8, 22 -- Reply-To: dac-at-research.umass.edu 8, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.9) Gecko/20071030 SeaMonkey/1.1.6 8, 22 -- MIME-Version: 1.0 8, 22 -- To: Yanling.Ge-at-tkk.fi, Microscopy Listserver {Microscopy-at-microscopy.com} 8, 22 -- Subject: Re: [Microscopy] viaWWW: about commercial plasma cleaner 8, 22 -- References: {200712171355.lBHDtSnK032458-at-ns.microscopy.com} 8, 22 -- In-Reply-To: {200712171355.lBHDtSnK032458-at-ns.microscopy.com} 8, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 22 -- Content-Transfer-Encoding: 7bit 8, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
==============================Original Headers============================== 10, 19 -- From scoyle-at-gatan.com Wed Dec 19 11:52:58 2007 10, 19 -- Received: from smtp.gatan.com (209342249.webadvantage.com [209.3.42.249] (may be forged)) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJHqvhV020412 10, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 11:52:58 -0600 10, 19 -- Received: from RNDLT10M65 ([10.13.0.163]) by smtp.gatan.com with Microsoft SMTPSVC(5.0.2195.6713); 10, 19 -- Wed, 19 Dec 2007 09:53:27 -0800 10, 19 -- From: "Steve Coyle" {scoyle-at-gatan.com} 10, 19 -- To: {Microscopy-at-microscopy.com} 10, 19 -- Subject: RE: viaWWW: about commercial plasma cleaner 10, 19 -- Date: Wed, 19 Dec 2007 09:52:56 -0800 10, 19 -- Message-ID: {038901c84267$fccd8050$0d070d0a-at-gatan.com} 10, 19 -- MIME-Version: 1.0 10, 19 -- Content-Type: text/plain; 10, 19 -- charset="us-ascii" 10, 19 -- Content-Transfer-Encoding: 7bit 10, 19 -- X-Mailer: Microsoft Office Outlook 11 10, 19 -- Thread-Index: AchBAIEuSe5ZqLw+QhaanGHkQ+ASUAAAK4EQAFmdeYA= 10, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 10, 19 -- X-OriginalArrivalTime: 19 Dec 2007 17:53:27.0743 (UTC) FILETIME=[0F19A4F0:01C84268] ==============================End of - Headers==============================
If you can get hold of a copy of the 1948 edition of the Metals Handbook (published by the ASM) you can find two pages of information on etching aluminum alloys (pp. 800-801). Probably similar information is contained in the more recent editions, too.
Some of the simpler etchants include: 0.5 ml HF in 99.5 ml water; 1 g NaOH in 99 ml water; 10 g NaOH in 90 ml water; 20 ml conc sulfuric acid in 80 ml water; 25 ml conc nitric acid in 75 ml water.
Have fun, life is short, WCB -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 3, 14 -- From bigelow-at-umich.edu Wed Dec 19 14:18:15 2007 3, 14 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81]) 3, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJKIFwH005531 3, 14 -- for {microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 14:18:15 -0600 3, 14 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 3, 14 -- BY hackers.mr.itd.umich.edu ID 47697C86.7F9FB.4303 ; 3, 14 -- 19 Dec 2007 15:18:14 -0500 3, 14 -- Mime-Version: 1.0 3, 14 -- Message-Id: {p06240800c38f2b18bea2-at-[141.212.131.221]} 3, 14 -- Date: Wed, 19 Dec 2007 15:18:12 -0500 3, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 3, 14 -- Subject: [Microscopy]RE:Etchants for Al 3, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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-----Original Message----- X-from: bigelow-at-umich.edu [mailto:bigelow-at-umich.edu] Sent: Wednesday, December 19, 2007 3:23 PM To: ph2-at-sprynet.com
If you can get hold of a copy of the 1948 edition of the Metals Handbook (published by the ASM) you can find two pages of information on etching aluminum alloys (pp. 800-801). Probably similar information is contained in the more recent editions, too.
Some of the simpler etchants include: 0.5 ml HF in 99.5 ml water; 1 g NaOH in 99 ml water; 10 g NaOH in 90 ml water; 20 ml conc sulfuric acid in 80 ml water; 25 ml conc nitric acid in 75 ml water.
Have fun, life is short, WCB -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 3, 14 -- From bigelow-at-umich.edu Wed Dec 19 14:18:15 2007 3, 14 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81]) 3, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJKIFwH005531 3, 14 -- for {microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 14:18:15 -0600 3, 14 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 3, 14 -- BY hackers.mr.itd.umich.edu ID 47697C86.7F9FB.4303 ; 3, 14 -- 19 Dec 2007 15:18:14 -0500 3, 14 -- Mime-Version: 1.0 3, 14 -- Message-Id: {p06240800c38f2b18bea2-at-[141.212.131.221]} 3, 14 -- Date: Wed, 19 Dec 2007 15:18:12 -0500 3, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 3, 14 -- Subject: [Microscopy]RE:Etchants for Al 3, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 19, 27 -- From ph2-at-sprynet.com Wed Dec 19 14:34:43 2007 19, 27 -- Received: from elasmtp-spurfowl.atl.sa.earthlink.net (elasmtp-spurfowl.atl.sa.earthlink.net [209.86.89.66]) 19, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJKYhku018346 19, 27 -- for {microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 14:34:43 -0600 19, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 19, 27 -- s=dk20050327; d=sprynet.com; 19, 27 -- b=ZFRaRIWrmgi1U0mim/Eys34nXtuzN6AqQTu0pBQcDmXMqSa6cnLuI3VaD2w7N3Um; 19, 27 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:In-Reply-To:X-MimeOLE:Message-ID:X-ELNK-Trace:X-Originating-IP; 19, 27 -- Received: from [75.61.18.94] (helo=user915fa8f284) 19, 27 -- by elasmtp-spurfowl.atl.sa.earthlink.net with asmtp (Exim 4.34) 19, 27 -- id 1J55co-0004dV-BJ 19, 27 -- for microscopy-at-microscopy.com; Wed, 19 Dec 2007 15:34:42 -0500 19, 27 -- From: "Tony Havics" {ph2-at-sprynet.com} 19, 27 -- To: "Microscopy Listserve" {microscopy-at-microscopy.com} 19, 27 -- Subject: RE: [Microscopy] RE:Etchants for Al 19, 27 -- Date: Wed, 19 Dec 2007 15:34:43 -0500 19, 27 -- MIME-Version: 1.0 19, 27 -- Content-Type: text/plain; 19, 27 -- charset="us-ascii" 19, 27 -- Content-Transfer-Encoding: 7bit 19, 27 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 19, 27 -- Thread-Index: AchCfP7na5WYwbbwRv6gdGVviFNWWgAASOqw 19, 27 -- In-Reply-To: {200712192023.lBJKNJHx013660-at-ns.microscopy.com} 19, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 19, 27 -- Message-ID: {E1J55co-0004dV-BJ-at-elasmtp-spurfowl.atl.sa.earthlink.net} 19, 27 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f9c18ff93e03c7ec4dc949fff41ef200d5350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 19, 27 -- X-Originating-IP: 75.61.18.94 ==============================End of - Headers==============================
Fluorescence Micrscopists and Vendors: I've been searching for actual published material to support the industry standard that requires igniting (turning on) mercury or xenon arc lamps PRIOR to turning on electronic microscopes or computer systems. Highly respectable microscopists have informed me that the electromagnetic fields (EMFs) produced by the intense 'welder's arc' spark can damage microchips and computer monitors nearby.
Does anyone have hard, fast evidence or direct experience to support this behavior? When consulting an information technology (IT) specialist about EMFs and computers, he says it will occasionally create problems on a computer monitor that is easily fixed by degaussing, and it can wipe temporary memory such as flash drives. Again, no permanent damage, but something to consider.
If so, how far would one need to keep the electronics from the arc lamp? I suspect that behavior is being dictated by a fear of the high expense in case there truly is a risk, but I don't want to perpetuate paranoia and extra lamp hours when not necessary. For example, my previous job didn't encourage the arc lamp being turned on first, and I don't recall any electronics issues attributed to such 'reckless behavior'.
Regards, ~Gregg
Gregg Sobocinski Imaging Specialist/Microscopist Univeristy of Michigan, MCDB Dept. Ann Arbor, Michigan USA
==============================Original Headers============================== 8, 25 -- From greggps-at-umich.edu Wed Dec 19 15:30:03 2007 8, 25 -- Received: from OWA02.adsroot.itcs.umich.edu (owa02.adsroot.itcs.umich.edu [141.211.27.139]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJLU3JH032436 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 15:30:03 -0600 8, 25 -- Received: from ECLUST2-VS3.adsroot.itcs.umich.edu ([141.211.27.143]) by OWA02.adsroot.itcs.umich.edu with Microsoft SMTPSVC(6.0.3790.1830); 8, 25 -- Wed, 19 Dec 2007 16:30:02 -0500 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 25 -- Content-class: urn:content-classes:message 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Type: text/plain; 8, 25 -- charset="us-ascii" 8, 25 -- Subject: Does arc lamp ignition really damage electronics via EMF emission? 8, 25 -- Date: Wed, 19 Dec 2007 16:29:57 -0500 8, 25 -- Message-ID: {3F08051AB847224F9B89DAB301137EF4023434F5-at-ECLUST2-VS3.adsroot.itcs.umich.edu} 8, 25 -- In-Reply-To: {200712120302.lBC32vmu030821-at-ns.microscopy.com} 8, 25 -- X-MS-Has-Attach: 8, 25 -- X-MS-TNEF-Correlator: 8, 25 -- Thread-Topic: Does arc lamp ignition really damage electronics via EMF emission? 8, 25 -- Thread-Index: Acg8a4B9gV/D7H65Rn6bCObApE8zxAF3MPtg 8, 25 -- References: {200712120302.lBC32vmu030821-at-ns.microscopy.com} 8, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} 8, 25 -- To: {microscopy-at-microscopy.com} 8, 25 -- X-OriginalArrivalTime: 19 Dec 2007 21:30:02.0965 (UTC) FILETIME=[50DB2850:01C84286] 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lBJLU3JH032436 ==============================End of - Headers==============================
Hi have been wondering about this. It seems that the EMF from starting the lamp is one possible reason for computer problems (if in- fact there are computer problems). Another problem could be in the power fluctuations that are propagated back into the house wiring. If this is the case, then the distance of lamp to computer would be less important that the shielding of the computer to bad power.
Any thoughts?
David
On Dec 19, 2007, at 2:33 PM, greggps-at-umich.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Fluorescence Micrscopists and Vendors: } I've been searching for actual published material to support the } industry standard that requires igniting (turning on) mercury or xenon } arc lamps PRIOR to turning on electronic microscopes or computer } systems. Highly respectable microscopists have informed me that the } electromagnetic fields (EMFs) produced by the intense 'welder's arc' } spark can damage microchips and computer monitors nearby. } } Does anyone have hard, fast evidence or direct experience to support } this behavior? When consulting an information technology (IT) } specialist } about EMFs and computers, he says it will occasionally create problems } on a computer monitor that is easily fixed by degaussing, and it can } wipe temporary memory such as flash drives. Again, no permanent } damage, } but something to consider. } } If so, how far would one need to keep the electronics from the arc } lamp? } I suspect that behavior is being dictated by a fear of the high } expense } in case there truly is a risk, but I don't want to perpetuate paranoia } and extra lamp hours when not necessary. For example, my previous job } didn't encourage the arc lamp being turned on first, and I don't } recall } any electronics issues attributed to such 'reckless behavior'. } } Regards, } ~Gregg } } Gregg Sobocinski } Imaging Specialist/Microscopist } Univeristy of Michigan, MCDB Dept. } Ann Arbor, Michigan } USA } } } } } ==============================Original } Headers============================== } 8, 25 -- From greggps-at-umich.edu Wed Dec 19 15:30:03 2007 } 8, 25 -- Received: from OWA02.adsroot.itcs.umich.edu } (owa02.adsroot.itcs.umich.edu [141.211.27.139]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id lBJLU3JH032436 } 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 } 15:30:03 -0600 } 8, 25 -- Received: from ECLUST2-VS3.adsroot.itcs.umich.edu } ([141.211.27.143]) by OWA02.adsroot.itcs.umich.edu with Microsoft } SMTPSVC(6.0.3790.1830); } 8, 25 -- Wed, 19 Dec 2007 16:30:02 -0500 } 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 8, 25 -- Content-class: urn:content-classes:message } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Type: text/plain; } 8, 25 -- charset="us-ascii" } 8, 25 -- Subject: Does arc lamp ignition really damage electronics } via EMF emission? } 8, 25 -- Date: Wed, 19 Dec 2007 16:29:57 -0500 } 8, 25 -- Message-ID: } {3F08051AB847224F9B89DAB301137EF4023434F5-at-ECLUST2- } VS3.adsroot.itcs.umich.edu} } 8, 25 -- In-Reply-To: {200712120302.lBC32vmu030821-at-ns.microscopy.com} } 8, 25 -- X-MS-Has-Attach: } 8, 25 -- X-MS-TNEF-Correlator: } 8, 25 -- Thread-Topic: Does arc lamp ignition really damage } electronics via EMF emission? } 8, 25 -- Thread-Index: Acg8a4B9gV/D7H65Rn6bCObApE8zxAF3MPtg } 8, 25 -- References: {200712120302.lBC32vmu030821-at-ns.microscopy.com} } 8, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} } 8, 25 -- To: {microscopy-at-microscopy.com} } 8, 25 -- X-OriginalArrivalTime: 19 Dec 2007 21:30:02.0965 (UTC) } FILETIME=[50DB2850:01C84286] } 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id lBJLU3JH032436 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 8, 22 -- From Elliott-at-arizona.edu Wed Dec 19 15:53:11 2007 8, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJLrBFE025201 8, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Dec 2007 15:53:11 -0600 8, 22 -- Received: from gandalfs_amavis (amavis4.email.arizona.edu [10.0.0.207]) 8, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 207B63BF23E 8, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Dec 2007 14:53:11 -0700 (MST) 8, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 8, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 98F1B3BCBB9 8, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Dec 2007 14:53:09 -0700 (MST) 8, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 8, 22 -- In-Reply-To: {200712192133.lBJLXFRN004754-at-ns.microscopy.com} 8, 22 -- References: {200712192133.lBJLXFRN004754-at-ns.microscopy.com} 8, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 8, 22 -- Message-Id: {CFE69380-78A4-4022-9A9E-9B5CEDC2EDBA-at-arizona.edu} 8, 22 -- Content-Transfer-Encoding: 7bit 8, 22 -- From: David Elliott {Elliott-at-arizona.edu} 8, 22 -- Subject: Re: [Microscopy] Does arc lamp ignition really damage electronics via EMF emission? 8, 22 -- Date: Wed, 19 Dec 2007 14:53:08 -0700 8, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 8, 22 -- X-Mailer: Apple Mail (2.752.2) 8, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Greg: I've got a UV fluorescence microscope (w/ Hg vapor lamp) in the same (small) room with both my SEMs and the flat panel monitor of the imaging computer is 6 inches away from the lamp housing; I've never noticed any interference or had any damage that I could track to the lamp arc. (Multi-users are another story.) We have the lamp on a timer that keeps it on about 10 hours a day, which is when most people here are likely to use the scope. I've been running the SEMs when it turns on and when it turns off and wouldn't have noticed if the timer hadn't clicked.
greggps-at-umich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Fluorescence Micrscopists and Vendors: } I've been searching for actual published material to support the } industry standard that requires igniting (turning on) mercury or xenon } arc lamps PRIOR to turning on electronic microscopes or computer } systems. Highly respectable microscopists have informed me that the } electromagnetic fields (EMFs) produced by the intense 'welder's arc' } spark can damage microchips and computer monitors nearby. } } Does anyone have hard, fast evidence or direct experience to support } this behavior? When consulting an information technology (IT) specialist } about EMFs and computers, he says it will occasionally create problems } on a computer monitor that is easily fixed by degaussing, and it can } wipe temporary memory such as flash drives. Again, no permanent damage, } but something to consider. } } If so, how far would one need to keep the electronics from the arc lamp? } I suspect that behavior is being dictated by a fear of the high expense } in case there truly is a risk, but I don't want to perpetuate paranoia } and extra lamp hours when not necessary. For example, my previous job } didn't encourage the arc lamp being turned on first, and I don't recall } any electronics issues attributed to such 'reckless behavior'. } } Regards, } ~Gregg } } Gregg Sobocinski } Imaging Specialist/Microscopist } Univeristy of Michigan, MCDB Dept. } Ann Arbor, Michigan } USA } } } } } ==============================Original Headers============================== } 8, 25 -- From greggps-at-umich.edu Wed Dec 19 15:30:03 2007 } 8, 25 -- Received: from OWA02.adsroot.itcs.umich.edu (owa02.adsroot.itcs.umich.edu [141.211.27.139]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJLU3JH032436 } 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 15:30:03 -0600 } 8, 25 -- Received: from ECLUST2-VS3.adsroot.itcs.umich.edu ([141.211.27.143]) by OWA02.adsroot.itcs.umich.edu with Microsoft SMTPSVC(6.0.3790.1830); } 8, 25 -- Wed, 19 Dec 2007 16:30:02 -0500 } 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 8, 25 -- Content-class: urn:content-classes:message } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Type: text/plain; } 8, 25 -- charset="us-ascii" } 8, 25 -- Subject: Does arc lamp ignition really damage electronics via EMF emission? } 8, 25 -- Date: Wed, 19 Dec 2007 16:29:57 -0500 } 8, 25 -- Message-ID: {3F08051AB847224F9B89DAB301137EF4023434F5-at-ECLUST2-VS3.adsroot.itcs.umich.edu} } 8, 25 -- In-Reply-To: {200712120302.lBC32vmu030821-at-ns.microscopy.com} } 8, 25 -- X-MS-Has-Attach: } 8, 25 -- X-MS-TNEF-Correlator: } 8, 25 -- Thread-Topic: Does arc lamp ignition really damage electronics via EMF emission? } 8, 25 -- Thread-Index: Acg8a4B9gV/D7H65Rn6bCObApE8zxAF3MPtg } 8, 25 -- References: {200712120302.lBC32vmu030821-at-ns.microscopy.com} } 8, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} } 8, 25 -- To: {microscopy-at-microscopy.com} } 8, 25 -- X-OriginalArrivalTime: 19 Dec 2007 21:30:02.0965 (UTC) FILETIME=[50DB2850:01C84286] } 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lBJLU3JH032436 } ==============================End of - Headers============================== } }
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 4, 24 -- From r-holdford-at-ti.com Wed Dec 19 16:03:54 2007 4, 24 -- Received: from calf.ext.ti.com (calf.ext.ti.com [198.47.26.144]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJM3rth005614 4, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Dec 2007 16:03:54 -0600 4, 24 -- Received: from dlep33.itg.ti.com ([157.170.170.112]) 4, 24 -- by calf.ext.ti.com (8.13.7/8.13.7) with ESMTP id lBJM3mQk013943 4, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 4, 24 -- Wed, 19 Dec 2007 16:03:53 -0600 4, 24 -- Received: from [156.117.194.120] (localhost [127.0.0.1]) 4, 24 -- by dlep33.itg.ti.com (8.13.7/8.13.7) with ESMTP id lBJM3lZw002544; 4, 24 -- Wed, 19 Dec 2007 16:03:47 -0600 (CST) 4, 24 -- Message-ID: {47699542.4090001-at-ti.com} 4, 24 -- Date: Wed, 19 Dec 2007 16:03:46 -0600 4, 24 -- From: Becky Holdford {r-holdford-at-ti.com} 4, 24 -- Organization: SC Packaging Development -- FA Development 4, 24 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 4, 24 -- MIME-Version: 1.0 4, 24 -- To: greggps-at-umich.edu, MSA ListServer {Microscopy-at-MSA.Microscopy.Com} 4, 24 -- Subject: Re: [Microscopy] Does arc lamp ignition really damage electronics 4, 24 -- via EMF emission? 4, 24 -- References: {200712192130.lBJLULeu032718-at-ns.microscopy.com} 4, 24 -- In-Reply-To: {200712192130.lBJLULeu032718-at-ns.microscopy.com} 4, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I worked in a facility that used a xenon flash tube. The electrical pulse to trigger the bright light was strong enough to put out a pulse that occasionally tripped some of the encoders on the system. During a typical manufacturing run the light flashed millions of time to draw a pattern on a photographic glass plate. Over a long period of time the position of devices was incorrect due to these EMP induced counts. The same may be true of laboratory equipment. In our case the absolute position values drifted. This caused a failure in the manufacturing procedure which was detected by a visual inspection system. Shielding and grounding of the electrical system that powered the bulb was sufficient to prevent these events from interfering with the rest of the equipment.
Now that 25 years has passed chip sets have smaller geometries that are packed tighter. This means less insulation between components and components less able to weather damage. A fatal 'nick' in the chip wiring is substantially smaller than it was 25 years ago.
I have no hard evidence of actual damage done to chips, but I do have first hand experience at seeing the effect of EMP on high precision equipment.
Robert Boehringer MS student Virginia Tech
==============================Original Headers============================== 4, 28 -- From rboehrin-at-vt.edu Wed Dec 19 16:25:21 2007 4, 28 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu [198.82.162.213]) 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJMPKCQ018466 4, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 16:25:20 -0600 4, 28 -- Received: from zidane.cc.vt.edu (evil-zidane.cc.vt.edu [10.1.1.13]) 4, 28 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id lBJMPI9j010989 4, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 17:25:19 -0500 4, 28 -- Received: from imp-b06.cc.vt.edu (imp.cc.vt.edu [198.82.161.55]) 4, 28 -- by zidane.cc.vt.edu (MOS 3.8.5-GA) 4, 28 -- with ESMTP id HZM32059; 4, 28 -- Wed, 19 Dec 2007 17:25:18 -0500 (EST) 4, 28 -- Received: from imp-b06.cc.vt.edu (localhost.localdomain [127.0.0.1]) 4, 28 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1) with ESMTP id lBJMPIek029769 4, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 17:25:18 -0500 4, 28 -- Received: (from apache-at-localhost) 4, 28 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1/Submit) id lBJMPH45029768 4, 28 -- for Microscopy-at-microscopy.com; Wed, 19 Dec 2007 17:25:17 -0500 4, 28 -- Received: from us194px00-pat.tycoelectronics.net (us194px00-pat.tycoelectronics.net [198.175.154.212]) 4, 28 -- by webmail.vt.edu (IMP) with HTTP; Wed, 19 Dec 2007 17:25:17 -0500 4, 28 -- Message-ID: {1198103117.47699a4ddf88b-at-webmail.vt.edu} 4, 28 -- Date: Wed, 19 Dec 2007 17:25:17 -0500 4, 28 -- From: Robert Boehringer {rboehrin-at-vt.edu} 4, 28 -- To: Microscopy-at-microscopy.com 4, 28 -- Subject: re: damage due to EMP 4, 28 -- MIME-Version: 1.0 4, 28 -- Content-Type: text/plain; charset=ISO-8859-1 4, 28 -- Content-Transfer-Encoding: 8bit 4, 28 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 ==============================End of - Headers==============================
Gregg, Video microscopy got going in the early 1980's with the inclusion of computers not far behind. In those days many people were using 200W lamps with massive (often old) powersupplies. These things hummed, banged, and sometimes dimmed the room lights when fired. I suspect the newer, slimmer units, 50 and 100W, have better behaved electronics, and cause less electrical stress. They certainly run cooler and quieter. There is also probably a wider use of surge protectors and related circuitry in computers. So I guess in the early days there may have been problems but I think now could be folklore. I have heard mercury arc explosion stories and even witnessed one, but never heard of, let alone seen an example of, a story where firing an arc lamp EMF'd a neighboring PC.
As ever, Tobias
} } } Hi have been wondering about this. It seems that the EMF from } starting the lamp is one possible reason for computer problems (if in- } fact there are computer problems). Another problem could be in the } power fluctuations that are propagated back into the house wiring. } If this is the case, then the distance of lamp to computer would be } less important that the shielding of the computer to bad power. } } Any thoughts? } } David } } } On Dec 19, 2007, at 2:33 PM, greggps-at-umich.edu wrote: } } } } } } } } } } ------ } } } } Fluorescence Micrscopists and Vendors: } } I've been searching for actual published material to support the } } industry standard that requires igniting (turning on) mercury or xenon } } arc lamps PRIOR to turning on electronic microscopes or computer } } systems. Highly respectable microscopists have informed me that the } } electromagnetic fields (EMFs) produced by the intense 'welder's arc' } } spark can damage microchips and computer monitors nearby. } } } } Does anyone have hard, fast evidence or direct experience to support } } this behavior? When consulting an information technology (IT) } } specialist } } about EMFs and computers, he says it will occasionally create problems } } on a computer monitor that is easily fixed by degaussing, and it can } } wipe temporary memory such as flash drives. Again, no permanent } } damage, } } but something to consider. } } } } If so, how far would one need to keep the electronics from the arc } } lamp? } } I suspect that behavior is being dictated by a fear of the high } } expense } } in case there truly is a risk, but I don't want to perpetuate paranoia } } and extra lamp hours when not necessary. For example, my previous job } } didn't encourage the arc lamp being turned on first, and I don't } } recall } } any electronics issues attributed to such 'reckless behavior'. } } } } Regards, } } ~Gregg } } } } Gregg Sobocinski } } Imaging Specialist/Microscopist } } Univeristy of Michigan, MCDB Dept. } } Ann Arbor, Michigan } } USA } } } } } } } } } } ==============================Original } } Headers============================== } } 8, 25 -- From greggps-at-umich.edu Wed Dec 19 15:30:03 2007 } } 8, 25 -- Received: from OWA02.adsroot.itcs.umich.edu } } (owa02.adsroot.itcs.umich.edu [141.211.27.139]) } } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id lBJLU3JH032436 } } 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 } } 15:30:03 -0600 } } 8, 25 -- Received: from ECLUST2-VS3.adsroot.itcs.umich.edu } } ([141.211.27.143]) by OWA02.adsroot.itcs.umich.edu with Microsoft } } SMTPSVC(6.0.3790.1830); } } 8, 25 -- Wed, 19 Dec 2007 16:30:02 -0500 } } 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } } 8, 25 -- Content-class: urn:content-classes:message } } 8, 25 -- MIME-Version: 1.0 } } 8, 25 -- Content-Type: text/plain; } } 8, 25 -- charset="us-ascii" } } 8, 25 -- Subject: Does arc lamp ignition really damage electronics } } via EMF emission? } } 8, 25 -- Date: Wed, 19 Dec 2007 16:29:57 -0500 } } 8, 25 -- Message-ID: } } {3F08051AB847224F9B89DAB301137EF4023434F5-at-ECLUST2- } } VS3.adsroot.itcs.umich.edu} } } 8, 25 -- In-Reply-To: {200712120302.lBC32vmu030821-at-ns.microscopy.com} } } 8, 25 -- X-MS-Has-Attach: } } 8, 25 -- X-MS-TNEF-Correlator: } } 8, 25 -- Thread-Topic: Does arc lamp ignition really damage } } electronics via EMF emission? } } 8, 25 -- Thread-Index: Acg8a4B9gV/D7H65Rn6bCObApE8zxAF3MPtg } } 8, 25 -- References: {200712120302.lBC32vmu030821-at-ns.microscopy.com} } } 8, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} } } 8, 25 -- To: {microscopy-at-microscopy.com} } } 8, 25 -- X-OriginalArrivalTime: 19 Dec 2007 21:30:02.0965 (UTC) } } FILETIME=[50DB2850:01C84286] } } 8, 25 -- Content-Transfer-Encoding: 8bit } } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id lBJLU3JH032436 } } ==============================End of - } } Headers============================== } } } } } ==============================Original Headers============================== } 8, 22 -- From Elliott-at-arizona.edu Wed Dec 19 15:53:11 2007 } 8, 22 -- Received: from smtpgate.email.arizona.edu } (gandalf.email.arizona.edu [128.196.133.169]) } 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id lBJLrBFE025201 } 8, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Dec 2007 } 15:53:11 -0600 } 8, 22 -- Received: from gandalfs_amavis (amavis4.email.arizona.edu } [10.0.0.207]) } 8, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id } 207B63BF23E } 8, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Dec 2007 } 14:53:11 -0700 (MST) } 8, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) } 8, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id } 98F1B3BCBB9 } 8, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Dec 2007 } 14:53:09 -0700 (MST) } 8, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) } 8, 22 -- In-Reply-To: {200712192133.lBJLXFRN004754-at-ns.microscopy.com} } 8, 22 -- References: {200712192133.lBJLXFRN004754-at-ns.microscopy.com} } 8, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed } 8, 22 -- Message-Id: {CFE69380-78A4-4022-9A9E-9B5CEDC2EDBA-at-arizona.edu} } 8, 22 -- Content-Transfer-Encoding: 7bit } 8, 22 -- From: David Elliott {Elliott-at-arizona.edu} } 8, 22 -- Subject: Re: [Microscopy] Does arc lamp ignition really } damage electronics via EMF emission? } 8, 22 -- Date: Wed, 19 Dec 2007 14:53:08 -0700 } 8, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} } 8, 22 -- X-Mailer: Apple Mail (2.752.2) } 8, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu } ==============================End of - Headers==============================
On Dec 18, 2007, at 6:12 PM, dsherman-at-purdue.edu wrote:
} I have a couple of general questions about the use of EELS in certain } experimental situations. I would appreciate comments on the } feasibility of } identifying elements using this technique in the following scenarios: } } In certain situations, cells can be influenced so that there is a } rapid } influx of Ca. This can be seen and documented using confocal } microscopy. } Would it be possible to capture the Ca++ distribution in the cells } with } greater resolution if cells were treated to induce the Ca++ influx } and then } immediately subjected to high pressure freezing? They would be FS } and resin } embedded prior to TEM analysis with EELS. } } There is a theory that, also under certain conditions, zinc will } enter the } cells via Ca++ channels. Would it be possible to pick up the Zn++ } using } EELS? } } Is it possible to simultaneously do a standard tomogram and also an } EELS } tomogram so that the two could be superimposed? } } Any references documenting transient ion flux using EELS would be } greatly } appreciated as would any comments from those experienced using the } technique.
Dear Debby, A. Somlyo was able to determine Ca in muscle cells, so detection is certainly possible, although there may be many technical difficulties with any particular system. Somlyo, for example, had to use a second- difference method to pull the Ca signal out of the noise. I am not sure of what happens to Ca, Zn, or, for that matter, other ions during freeze-substitution, but if they stay put, then EELS could pick them up. There is little difference between Ca and Zn, although Zn edges may be either more or less visible than Ca due to energy difference, interference with the stain atoms, or other problems. Rather than do simultaneous tomograms, which could be done from position-tagged spectrum collection (a good feature of the Zeiss 912, but maybe not available on all scopes), I would take a regular tilt series, then make a stereo pair at the Ca and/or Zn edge--you'd have to subtract background, so each image would require two or three exposures, and the dose for good signal-to-noise would be higher than for a zero-loss image. It is this requirement which would increase the total dose necessary to get good EELS mapping and a tilt series to the point where you might damage even a plastic section. You could then use Sterecon, or some other program to get a 3D EELS map from the stereo pair to match up with the higher resolution tomogram. Good luck. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Wed Dec 19 17:49:41 2007 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJNnfs1012764 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 19 Dec 2007 17:49:41 -0600 6, 22 -- Received: from earth-dog.its.caltech.edu (earth-dog [192.168.1.3]) 6, 22 -- by earth-ox-postvirus (Postfix) with ESMTP id 368501BDA7 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 19 Dec 2007 15:49:40 -0800 (PST) 6, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 6, 22 -- by wood-ox.its.caltech.edu (Postfix) with ESMTP id AAEE813ED3 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 19 Dec 2007 15:49:37 -0800 (PST) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v752.3) 6, 22 -- In-Reply-To: {200712190212.lBJ2CZvw014496-at-ns.microscopy.com} 6, 22 -- References: {200712190212.lBJ2CZvw014496-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 22 -- Message-Id: {25F7F6CF-EC89-42D7-A390-D7D6A0AF8A2A-at-caltech.edu} 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- Subject: Re: [Microscopy] EELS information 6, 22 -- Date: Wed, 19 Dec 2007 15:49:42 -0800 6, 22 -- To: microscopy-at-msa.microscopy.com 6, 22 -- X-Mailer: Apple Mail (2.752.3) 6, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.4.5 ==============================End of - Headers==============================
A few remarks on this topic (sorry for not having the time to look up all details).
Two people came into my mind, when reading Debby's mail and thinking about the problem.
1. the late Karl Zierold (MPI Dortmund) was able to show that for this type of question, rapid freezing and then imaging the freeze-dried cryo-sections (he used - not only? - the STEM) is an adequate way for tackling this problem. Karl was not able to use tomography, though. - The question is why not using freeze-dried (or frozen-hydrated) cryo-sections, rather than going to FS and embedding in resin? and then also trying cryo-fluorescence light microscopy (Anna Sartori comes into my mind)
(yes , I know, the high e-dose for EELS is a severe problem and most likely limiting)
Looking up Karl's papers and contributions will help to clarify details which are not in my mind.
2. Helmut Plattner and his group also worked on this topic. I recall a talk by Helmut where he showed convincingly that in biology, some 'events' have been observed (e.g. on excitable membranes) which are considerably (orders of magnitude) faster than 'rapid' freezing in a high-pressure freezer (or other freezing devices). In these cases, HPF is possible of course, but might be too slow, to capture the event rapidly enough.
best regards,
Reinhard
--
---------------------- PD Dr. Reinhard Rachel Universitaet Regensburg Centre for EM - NWF III - -at-Institute for Anatomy Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-r.de office: VKL 3.1.29
==============================Original Headers============================== 12, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Thu Dec 20 02:11:21 2007 12, 25 -- Received: from rrzmta1.rz.uni-regensburg.de (rrzmta1.rz.uni-regensburg.de [194.94.155.51]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBK8BLrx005288 12, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 20 Dec 2007 02:11:21 -0600 12, 25 -- Received: from rrzmta1.rz.uni-regensburg.de (localhost [127.0.0.1]) 12, 25 -- by localhost (Postfix) with SMTP id D8F3C505E0 12, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 20 Dec 2007 09:11:22 +0100 (CET) 12, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.rz.uni-regensburg.de [132.199.4.80]) 12, 25 -- by rrzmta1.rz.uni-regensburg.de (Postfix) with ESMTP id D2EF0505A8 12, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 20 Dec 2007 09:11:22 +0100 (CET) 12, 25 -- Received: from uni-regensburg-MTA by gwsmtp1.uni-regensburg.de 12, 25 -- with Novell_GroupWise; Thu, 20 Dec 2007 09:10:35 +0100 12, 25 -- Message-Id: {476A31B3.044C.0054.0-at-biologie.uni-regensburg.de} 12, 25 -- X-Mailer: Novell GroupWise Internet Agent 7.0.2 HP 12, 25 -- Date: Thu, 20 Dec 2007 09:11:15 +0100 12, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 12, 25 -- To: {Microscopy-at-Microscopy.Com} 12, 25 -- Subject: Antw: [Microscopy] EELS information 12, 25 -- References: {200712192350.lBJNo0fL013178-at-ns.microscopy.com} 12, 25 -- In-Reply-To: {200712192350.lBJNo0fL013178-at-ns.microscopy.com} 12, 25 -- Mime-Version: 1.0 12, 25 -- Content-Type: text/plain; charset=US-ASCII 12, 25 -- Content-Disposition: inline 12, 25 -- Content-Transfer-Encoding: 8bit 12, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lBK8BLrx005288 ==============================End of - Headers==============================
The Bio-Rad and Leica engineers have repeatedly told me that switching on the arc lamp supply can wipe out a diode laser on a confocal system (the argon ones are tougher apparently) - so I always switch them off first (although with a £13k p.a. maintenance contract I suppose I'm only saving the manufacturer money). My father once left his computer on by mistake when an electrician was wiring a new plug socket in his house. The electrician switched on/off the mains three or four times - the PC went off first time and stayed off safe - but his rather nice 19" Dell CRT monitor naturally stayed hard switched on - it was completely dead by the time my dad realized the PC was still on, just out of warranty and went into the bin (£350 for a new one).
I use surge protectors on all HIFI/TV and computers and have never lost any equipment - there is no surge protectors in PCs [or much else] and all fuses are hard wired into the motherboard so if one fails it's a new PC [a few modern ones have a few resettable fuses]. Fortunately the PC power supply is modular and can be replaced easily if that fails. My mother-in-law doesn't have mains surge protectors and has lost a quite new Panasonic DVD player, TV and HiFi Marantz amp over the last 5 years - all due to mains transformer failure (they all went in the bin, Marantz wanted well over £100 for their new transformer). My last [Sugden A48II HiFi] amp, so ugly it must have been expensive, lasted 25 years and only went to the bin because the electronic components were simply getting too old and noisy at low volume - and it still played loud perfectly, although I admit it was built like a Challenger tank.
Of course any lightening strike nearby and no surge protection and you will lose a lot of equipment (we lost many PCs and the odd lab device when our lab building was struck by lightening back in the 80s). I have had quite a few mains protectors fail though - they take the hit instead of your electronics. So they are made/sold for a reason - many manufacturers state only switch on/off the device using the internal switch not the wall socket one (as it's inferior in quality and so more noisy) although that's assuming the manufacture bothered to fit an on/off switch in the first place.
However that said, I have never known a PC or similar fail when switching on/off an arc lamp. I tend to put a surge protector on the arc lamp controller mains-line though.
Dr Keith J Morris Molecular Cytogenetics and Microscopy Core The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom
-----Original Message----- X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] Sent: 19 December 2007 22:40 To: kjmorris-at-well.ox.ac.uk
Gregg, Video microscopy got going in the early 1980's with the inclusion of computers not far behind. In those days many people were using 200W lamps with massive (often old) powersupplies. These things hummed, banged, and sometimes dimmed the room lights when fired. I suspect the newer, slimmer units, 50 and 100W, have better behaved electronics, and cause less electrical stress. They certainly run cooler and quieter. There is also probably a wider use of surge protectors and related circuitry in computers. So I guess in the early days there may have been problems but I think now could be folklore. I have heard mercury arc explosion stories and even witnessed one, but never heard of, let alone seen an example of, a story where firing an arc lamp EMF'd a neighboring PC.
As ever, Tobias
} } } Hi have been wondering about this. It seems that the EMF from } starting the lamp is one possible reason for computer problems (if in- } fact there are computer problems). Another problem could be in the } power fluctuations that are propagated back into the house wiring. } If this is the case, then the distance of lamp to computer would be } less important that the shielding of the computer to bad power. } } Any thoughts? } } David } } } On Dec 19, 2007, at 2:33 PM, greggps-at-umich.edu wrote: } } } } } } } } } } ------ } } } } Fluorescence Micrscopists and Vendors: } } I've been searching for actual published material to support the } } industry standard that requires igniting (turning on) mercury or xenon } } arc lamps PRIOR to turning on electronic microscopes or computer } } systems. Highly respectable microscopists have informed me that the } } electromagnetic fields (EMFs) produced by the intense 'welder's arc' } } spark can damage microchips and computer monitors nearby. } } } } Does anyone have hard, fast evidence or direct experience to support } } this behavior? When consulting an information technology (IT) } } specialist } } about EMFs and computers, he says it will occasionally create problems } } on a computer monitor that is easily fixed by degaussing, and it can } } wipe temporary memory such as flash drives. Again, no permanent } } damage, } } but something to consider. } } } } If so, how far would one need to keep the electronics from the arc } } lamp? } } I suspect that behavior is being dictated by a fear of the high } } expense } } in case there truly is a risk, but I don't want to perpetuate paranoia } } and extra lamp hours when not necessary. For example, my previous job } } didn't encourage the arc lamp being turned on first, and I don't } } recall } } any electronics issues attributed to such 'reckless behavior'. } } } } Regards, } } ~Gregg } } } } Gregg Sobocinski } } Imaging Specialist/Microscopist } } Univeristy of Michigan, MCDB Dept. } } Ann Arbor, Michigan } } USA } } } } } } } } } } ==============================Original } } Headers============================== } } 8, 25 -- From greggps-at-umich.edu Wed Dec 19 15:30:03 2007 } } 8, 25 -- Received: from OWA02.adsroot.itcs.umich.edu } } (owa02.adsroot.itcs.umich.edu [141.211.27.139]) } } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id lBJLU3JH032436 } } 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 } } 15:30:03 -0600 } } 8, 25 -- Received: from ECLUST2-VS3.adsroot.itcs.umich.edu } } ([141.211.27.143]) by OWA02.adsroot.itcs.umich.edu with Microsoft } } SMTPSVC(6.0.3790.1830); } } 8, 25 -- Wed, 19 Dec 2007 16:30:02 -0500 } } 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } } 8, 25 -- Content-class: urn:content-classes:message } } 8, 25 -- MIME-Version: 1.0 } } 8, 25 -- Content-Type: text/plain; } } 8, 25 -- charset="us-ascii" } } 8, 25 -- Subject: Does arc lamp ignition really damage electronics } } via EMF emission? } } 8, 25 -- Date: Wed, 19 Dec 2007 16:29:57 -0500 } } 8, 25 -- Message-ID: } } {3F08051AB847224F9B89DAB301137EF4023434F5-at-ECLUST2- } } VS3.adsroot.itcs.umich.edu} } } 8, 25 -- In-Reply-To: {200712120302.lBC32vmu030821-at-ns.microscopy.com} } } 8, 25 -- X-MS-Has-Attach: } } 8, 25 -- X-MS-TNEF-Correlator: } } 8, 25 -- Thread-Topic: Does arc lamp ignition really damage } } electronics via EMF emission? } } 8, 25 -- Thread-Index: Acg8a4B9gV/D7H65Rn6bCObApE8zxAF3MPtg } } 8, 25 -- References: {200712120302.lBC32vmu030821-at-ns.microscopy.com} } } 8, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} } } 8, 25 -- To: {microscopy-at-microscopy.com} } } 8, 25 -- X-OriginalArrivalTime: 19 Dec 2007 21:30:02.0965 (UTC) } } FILETIME=[50DB2850:01C84286] } } 8, 25 -- Content-Transfer-Encoding: 8bit } } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id lBJLU3JH032436 } } ==============================End of - } } Headers============================== } } } } } ==============================Original Headers============================== } 8, 22 -- From Elliott-at-arizona.edu Wed Dec 19 15:53:11 2007 } 8, 22 -- Received: from smtpgate.email.arizona.edu } (gandalf.email.arizona.edu [128.196.133.169]) } 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id lBJLrBFE025201 } 8, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Dec 2007 } 15:53:11 -0600 } 8, 22 -- Received: from gandalfs_amavis (amavis4.email.arizona.edu } [10.0.0.207]) } 8, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id } 207B63BF23E } 8, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Dec 2007 } 14:53:11 -0700 (MST) } 8, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) } 8, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id } 98F1B3BCBB9 } 8, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 19 Dec 2007 } 14:53:09 -0700 (MST) } 8, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) } 8, 22 -- In-Reply-To: {200712192133.lBJLXFRN004754-at-ns.microscopy.com} } 8, 22 -- References: {200712192133.lBJLXFRN004754-at-ns.microscopy.com} } 8, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed } 8, 22 -- Message-Id: {CFE69380-78A4-4022-9A9E-9B5CEDC2EDBA-at-arizona.edu} } 8, 22 -- Content-Transfer-Encoding: 7bit } 8, 22 -- From: David Elliott {Elliott-at-arizona.edu} } 8, 22 -- Subject: Re: [Microscopy] Does arc lamp ignition really } damage electronics via EMF emission? } 8, 22 -- Date: Wed, 19 Dec 2007 14:53:08 -0700 } 8, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} } 8, 22 -- X-Mailer: Apple Mail (2.752.2) } 8, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu } ==============================End of - Headers==============================
It will be dificult to find a direct tunnig between the two methods. The dept scale are too much different. To make a comparaison, one should make XPS depth profiling, suposing that the ion gun etching will no modify the composition of the sample.
We have tried a lot, between XPS, AES, RBS and X ray reflectometry, and we never get a direct accord. One get different sets of results, and one have to weight them with all one know about the samples, and decides where is the right (or the less wrong) line between them.
About the RBS, it schould not be destructive , but as someone has soon said, it depends of your samples. The destrucive effect may be surprising ! I had once a set of samples to observe with the SEM, after they were "treated" by RBS. They were covered with a very thick layer of carbon (tenth of nm) due to contamination by the poor vaccum of the RBS vessel, and induced by the He+ beam ! For our purpous, it was "destructive" !
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
andreana782-at-yahoo.com.tw a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both andreana782-at-yahoo.com.tw as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: andreana782-at-yahoo.com.tw } Name: Jean Chen } } Title-Subject: [Filtered] elemental composition } } Question: } I was wondering if anyone can give a comment on which analytical tool can give the best composition ratio of the solid materials. As far as I know, RBS can determine the composition of bulk sample. XPS can determine the composition of the surface. Does anyone know which one can give a more acurate composition? By the way, RBS should be a non-destructive tool or a destructive tool? } } Thank you! } } Login Host: 71.204.147.55 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Wed Dec 19 07:53:52 2007 } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJDrpRd026557 } 6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 07:53:51 -0600 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240801c38ed2db0cac-at-[206.69.208.22]} } 6, 11 -- Date: Wed, 19 Dec 2007 07:53:50 -0600 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: andreana782-at-yahoo.com.tw (by way of MicroscopyListserver) } 6, 11 -- Subject: viaWWW: elemental composition measurements } 6, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 10, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Thu Dec 20 08:04:59 2007 10, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.151]) 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBKE4v5m010905 10, 29 -- for {microscopy-at-microscopy.com} ; Thu, 20 Dec 2007 08:04:58 -0600 10, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 10, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id lBKE4sG4032109 10, 29 -- ; Thu, 20 Dec 2007 15:04:54 +0100 (CET) 10, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 10, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 76B9C1077FFF; 10, 29 -- Thu, 20 Dec 2007 15:04:15 +0100 (CET) 10, 29 -- Message-ID: {476A7676.6090608-at-ipcms.u-strasbg.fr} 10, 29 -- Date: Thu, 20 Dec 2007 15:04:38 +0100 10, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 10, 29 -- User-Agent: Thunderbird 1.5.0.14pre (X11/20071022) 10, 29 -- MIME-Version: 1.0 10, 29 -- To: andreana782-at-yahoo.com.tw, Microscopy-at-microscopy.com 10, 29 -- Subject: Re: [Microscopy] viaWWW: elemental composition measurements 10, 29 -- References: {200712191359.lBJDxqfx002640-at-ns.microscopy.com} 10, 29 -- In-Reply-To: {200712191359.lBJDxqfx002640-at-ns.microscopy.com} 10, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 29 -- Content-Transfer-Encoding: 8bit 10, 29 -- X-IPCMS-MailScanner: Found to be clean 10, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 10, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.151]); Thu, 20 Dec 2007 15:04:54 +0100 (CET) 10, 29 -- X-Virus-Scanned: ClamAV 0.88.7/5186/Thu Dec 20 04:38:25 2007 on mr1.u-strasbg.fr 10, 29 -- X-Virus-Status: Clean 10, 29 -- X-Spam-Status: No, score=0.0 required=5.0 tests=AWL,MAILTO_TO_SPAM_ADDR 10, 29 -- autolearn=disabled version=3.1.8 10, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr1.u-strasbg.fr ==============================End of - Headers==============================
This is not hard evidence about EMP damage, but the type of lamp, type of housing (internal/external ignitor?) and type of ignition certainly affect the POTENTIAL for problems.
First, let's assume that no one is still using the old units with spark-gaps.......
Second, Hg and Xe(Hg) lamps breakover at about 4kV and I have seen some mention of "soft start" but don't know about that. The current surge through the cable following breakover is what is most likely to radiate a pulse.
Xenon lamps with no mercury included break over at a much higher voltage (ignitors for Xe typically put out 20kV) and the start pulse typically has more energy as well.
I have run a confocal system in close proximity to HBO-100 Hg lamps (Nikon supplied) for years and saw no effects (keeping the arc lamp cable well away from other cables to be safe....). We also had an SGI server in the room that ran 24hr and it never had a problem.
Dale
greggps-at-umich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Fluorescence Micrscopists and Vendors: } I've been searching for actual published material to support the } industry standard that requires igniting (turning on) mercury or xenon } arc lamps PRIOR to turning on electronic microscopes or computer } systems. Highly respectable microscopists have informed me that the } electromagnetic fields (EMFs) produced by the intense 'welder's arc' } spark can damage microchips and computer monitors nearby. } } Does anyone have hard, fast evidence or direct experience to support } this behavior? When consulting an information technology (IT) specialist } about EMFs and computers, he says it will occasionally create problems } on a computer monitor that is easily fixed by degaussing, and it can } wipe temporary memory such as flash drives. Again, no permanent damage, } but something to consider. } } If so, how far would one need to keep the electronics from the arc lamp? } I suspect that behavior is being dictated by a fear of the high expense } in case there truly is a risk, but I don't want to perpetuate paranoia } and extra lamp hours when not necessary. For example, my previous job } didn't encourage the arc lamp being turned on first, and I don't recall } any electronics issues attributed to such 'reckless behavior'. } } Regards, } ~Gregg } } Gregg Sobocinski } Imaging Specialist/Microscopist } Univeristy of Michigan, MCDB Dept. } Ann Arbor, Michigan } USA } } } } } ==============================Original Headers============================== } 8, 25 -- From greggps-at-umich.edu Wed Dec 19 15:30:03 2007 } 8, 25 -- Received: from OWA02.adsroot.itcs.umich.edu (owa02.adsroot.itcs.umich.edu [141.211.27.139]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJLU3JH032436 } 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 15:30:03 -0600 } 8, 25 -- Received: from ECLUST2-VS3.adsroot.itcs.umich.edu ([141.211.27.143]) by OWA02.adsroot.itcs.umich.edu with Microsoft SMTPSVC(6.0.3790.1830); } 8, 25 -- Wed, 19 Dec 2007 16:30:02 -0500 } 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 8, 25 -- Content-class: urn:content-classes:message } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Type: text/plain; } 8, 25 -- charset="us-ascii" } 8, 25 -- Subject: Does arc lamp ignition really damage electronics via EMF emission? } 8, 25 -- Date: Wed, 19 Dec 2007 16:29:57 -0500 } 8, 25 -- Message-ID: {3F08051AB847224F9B89DAB301137EF4023434F5-at-ECLUST2-VS3.adsroot.itcs.umich.edu} } 8, 25 -- In-Reply-To: {200712120302.lBC32vmu030821-at-ns.microscopy.com} } 8, 25 -- X-MS-Has-Attach: } 8, 25 -- X-MS-TNEF-Correlator: } 8, 25 -- Thread-Topic: Does arc lamp ignition really damage electronics via EMF emission? } 8, 25 -- Thread-Index: Acg8a4B9gV/D7H65Rn6bCObApE8zxAF3MPtg } 8, 25 -- References: {200712120302.lBC32vmu030821-at-ns.microscopy.com} } 8, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} } 8, 25 -- To: {microscopy-at-microscopy.com} } 8, 25 -- X-OriginalArrivalTime: 19 Dec 2007 21:30:02.0965 (UTC) FILETIME=[50DB2850:01C84286] } 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lBJLU3JH032436 } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 23 -- From dac-at-research.umass.edu Thu Dec 20 09:54:31 2007 9, 23 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBKFsU5U026970 9, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Dec 2007 09:54:31 -0600 9, 23 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 9, 23 -- (authenticated bits=0) 9, 23 -- by race5.oit.umass.edu (8.14.1/8.14.1) with ESMTP id lBKFsU45017487 9, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 9, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Dec 2007 10:54:30 -0500 9, 23 -- Message-ID: {476A90AB.6080902-at-research.umass.edu} 9, 23 -- Date: Thu, 20 Dec 2007 10:56:27 -0500 9, 23 -- From: Dale Callaham {dac-at-research.umass.edu} 9, 23 -- Reply-To: dac-at-research.umass.edu 9, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.11) Gecko/20071128 SeaMonkey/1.1.7 9, 23 -- MIME-Version: 1.0 9, 23 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 9, 23 -- Subject: Re: [Microscopy] Does arc lamp ignition really damage electronics 9, 23 -- via EMF emission? 9, 23 -- References: {200712192137.lBJLbP1g016769-at-ns.microscopy.com} 9, 23 -- In-Reply-To: {200712192137.lBJLbP1g016769-at-ns.microscopy.com} 9, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 23 -- Content-Transfer-Encoding: 7bit 9, 23 -- X-Whitelist: TRUE ==============================End of - Headers==============================
I have a researcher here who needs to remove some gold palladium coatings from some insect samples. He was successful in removing the gold coatings, but the reference we found from the 70's using a FeCl3 solution in alcohol has not worked- at least on coated coverslips.
Do any of you have experience removing an au/pd coating from delicate biologic tissues you would be willing to pass along?
Thanks,
Scott Whittaker Head NMNH Imaging Manager SEM Lab Smithsonian Institution National Museum of Natural History PO Box 37012 MRC104 Washington DC 20013-7012 202-633-0891
==============================Original Headers============================== 10, 26 -- From WHITTAKS-at-si.edu Thu Dec 20 10:41:11 2007 10, 26 -- Received: from si-mailout04.si.edu (si-mailout04.si.edu [160.111.103.178]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBKGfArH008390 10, 26 -- for {microscopy-at-microscopy.com} ; Thu, 20 Dec 2007 10:41:10 -0600 10, 26 -- Received: from si-msesmtpo-01.US.SINET.SI.EDU (SI-MSESMTP-N1.us.sinet.si.edu [160.111.49.75]) 10, 26 -- by si-mailout04.si.edu (Postfix) with ESMTP id 3B45A6B0B 10, 26 -- for {microscopy-at-microscopy.com} ; Thu, 20 Dec 2007 11:39:44 -0500 (EST) 10, 26 -- Received: from SI-ECL02.US.SINET.SI.EDU ([160.111.49.26]) by si-msesmtpo-01.US.SINET.SI.EDU with Microsoft SMTPSVC(6.0.3790.3959); 10, 26 -- Thu, 20 Dec 2007 11:40:31 -0500 10, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 26 -- Content-class: urn:content-classes:message 10, 26 -- MIME-Version: 1.0 10, 26 -- Content-Type: text/plain; 10, 26 -- charset="us-ascii" 10, 26 -- Subject: SEM- removing Au/Pd coatings 10, 26 -- Date: Thu, 20 Dec 2007 11:40:30 -0500 10, 26 -- Message-ID: {20CDD1CED2E76541A4FA119C6C806BA301EDA36B-at-SI-ECL02.US.SINET.SI.EDU} 10, 26 -- X-MS-Has-Attach: 10, 26 -- X-MS-TNEF-Correlator: 10, 26 -- Thread-Topic: SEM- removing Au/Pd coatings 10, 26 -- Thread-Index: AchDJwjkpYEuow6yR8uirWwqIrsu+Q== 10, 26 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu} 10, 26 -- To: {microscopy-at-microscopy.com} 10, 26 -- X-OriginalArrivalTime: 20 Dec 2007 16:40:31.0710 (UTC) FILETIME=[0931BFE0:01C84327] 10, 26 -- Content-Transfer-Encoding: 8bit 10, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id lBKGfArH008390 ==============================End of - Headers==============================
I suppose Nikon Microscopes can be trusted when they say:
"As digital imaging workstations become more popular and microscopes equipped with high-end camera systems grow more complex, it is important to remember how dangerous the arc lamp power supply can be to electronic equipment. Always turn the arc lamp on before powering on auxiliary computer or camera equipment that is in close proximity to the power supply, and always turn this equipment off before turning off the arc lamp. The cable supplying current to the lamp from the power supply is generally quite well shielded, but a momentary 20,000 to 50,000 volt surge is possible when the lamp is fired. This large voltage can generate a magnetic field strong enough to damage sensitive integrated circuits that are nearby."
I had always assumed that the faraday cage (metal box) around the mercury lamp and power supply shielded other equipment quite well from electro-magnetic pulses and that mains spikes from the power surge as the lamp starts up were the main problem*. That said I've always followed the microscopists aural tradition by intoning the dogma to users that "the mercury lamp goes on first and off last", although what about the other two imaging microscopes in the same room that are already in use? - I just hope that they aren't 'nearby' enough to suffer. Ironically I have always found the PCs and cameras on microscopes far more reliable than their cousins in the office and home.
I do notice that often lights dim, TV screens fluctuate etc.. when you switch on high current devices (e.g 8kW showers at home), but that's obviously not due to EMP. At UCL our 18W Spectra Physics laser draws 53kW from the mains but has no discernable effect at all on lighting, PC screens etc.. when we switch it on, probably as it's isolated behind it's own personal three phase power supply. Plus of course there is RF interference** that plagues TV, CRT and radio reception, that can be caused by switches, motors etc..., but that is also rather unlikely to kill off a PC or TV, although it isn't CRT and loudspeaker friendly (indeed audio noise from a weak signal to a stereo TV/Radio decoder can often sound worse as it switches repeatedly in and out). In comparison I've never noticed a mercury lamp EMP produce any effect whatsoever on nearby equipment (fortunately).
Keith
*I suppose I am more concerned about EMP from a air-burst atomic bomb explosion, if our lab is unfortunate enough to be less than 10km from it (or as Edward G Robinson put it "Sweet Mother of Mercy is this the end of our confocal?").
Dr Keith J Morris Molecular Cytogenetics and Microscopy Core The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom
-----Original Message----- X-from: rboehrin-at-vt.edu [mailto:rboehrin-at-vt.edu] Sent: 19 December 2007 22:34 To: kjmorris-at-well.ox.ac.uk
I worked in a facility that used a xenon flash tube. The electrical pulse to trigger the bright light was strong enough to put out a pulse that occasionally tripped some of the encoders on the system. During a typical manufacturing run the light flashed millions of time to draw a pattern on a photographic glass plate. Over a long period of time the position of devices was incorrect due to these EMP induced counts. The same may be true of laboratory equipment. In our case the absolute position values drifted. This caused a failure in the manufacturing procedure which was detected by a visual inspection system. Shielding and grounding of the electrical system that powered the bulb was sufficient to prevent these events from interfering with the rest of the equipment.
Now that 25 years has passed chip sets have smaller geometries that are packed tighter. This means less insulation between components and components less able to weather damage. A fatal 'nick' in the chip wiring is substantially smaller than it was 25 years ago.
I have no hard evidence of actual damage done to chips, but I do have first hand experience at seeing the effect of EMP on high precision equipment.
Robert Boehringer MS student Virginia Tech
==============================Original Headers============================== 4, 28 -- From rboehrin-at-vt.edu Wed Dec 19 16:25:21 2007 4, 28 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu [198.82.162.213]) 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBJMPKCQ018466 4, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 16:25:20 -0600 4, 28 -- Received: from zidane.cc.vt.edu (evil-zidane.cc.vt.edu [10.1.1.13]) 4, 28 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id lBJMPI9j010989 4, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 17:25:19 -0500 4, 28 -- Received: from imp-b06.cc.vt.edu (imp.cc.vt.edu [198.82.161.55]) 4, 28 -- by zidane.cc.vt.edu (MOS 3.8.5-GA) 4, 28 -- with ESMTP id HZM32059; 4, 28 -- Wed, 19 Dec 2007 17:25:18 -0500 (EST) 4, 28 -- Received: from imp-b06.cc.vt.edu (localhost.localdomain [127.0.0.1]) 4, 28 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1) with ESMTP id lBJMPIek029769 4, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Dec 2007 17:25:18 -0500 4, 28 -- Received: (from apache-at-localhost) 4, 28 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1/Submit) id lBJMPH45029768 4, 28 -- for Microscopy-at-microscopy.com; Wed, 19 Dec 2007 17:25:17 -0500 4, 28 -- Received: from us194px00-pat.tycoelectronics.net (us194px00-pat.tycoelectronics.net [198.175.154.212]) 4, 28 -- by webmail.vt.edu (IMP) with HTTP; Wed, 19 Dec 2007 17:25:17 -0500 4, 28 -- Message-ID: {1198103117.47699a4ddf88b-at-webmail.vt.edu} 4, 28 -- Date: Wed, 19 Dec 2007 17:25:17 -0500 4, 28 -- From: Robert Boehringer {rboehrin-at-vt.edu} 4, 28 -- To: Microscopy-at-microscopy.com 4, 28 -- Subject: re: damage due to EMP 4, 28 -- MIME-Version: 1.0 4, 28 -- Content-Type: text/plain; charset=ISO-8859-1 4, 28 -- Content-Transfer-Encoding: 8bit 4, 28 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 ==============================End of - Headers==============================
==============================Original Headers============================== 30, 20 -- From kjmorris-at-well.ox.ac.uk Fri Dec 21 03:17:24 2007 30, 20 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 30, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBL9HNaY018634 30, 20 -- for {microscopy-at-microscopy.com} ; Fri, 21 Dec 2007 03:17:23 -0600 30, 20 -- Received: from dhcp507.well.ox.ac.uk ([129.67.46.94] helo=wellvolocity) 30, 20 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 30, 20 -- id 1J5e0R-0001fH-49 30, 20 -- for microscopy-at-microscopy.com; Fri, 21 Dec 2007 09:17:23 +0000 30, 20 -- From: "Keith J Morris" {kjmorris-at-well.ox.ac.uk} 30, 20 -- To: {microscopy-at-microscopy.com} 30, 20 -- Subject: FW: [Microscopy] re: damage due to EMP 30, 20 -- Date: Fri, 21 Dec 2007 09:17:49 -0000 30, 20 -- Message-ID: {000c01c843b2$5b89db20$5e2e4381-at-wellvolocity} 30, 20 -- MIME-Version: 1.0 30, 20 -- Content-Type: text/plain; 30, 20 -- charset="US-ASCII" 30, 20 -- Content-Transfer-Encoding: 7bit 30, 20 -- X-Mailer: Microsoft Office Outlook 11 30, 20 -- Thread-Index: AchCjzLL2V0QM8M4TIGC4M7nySTlwwAg9mAgAAU1j5AAA92t0AAC9s3gABtijCA= 30, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
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Email: opmills-at-mtu.edu Name: Owen Mills
Organization: Michigan Tech University
Title-Subject: [Filtered] Pelco Model "H" CP dryer
Question: Hi,
Are any of you still using this machine? I want to make sure it is safe. Next, do any of you have a manual I can copy?
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Email: panxijiang-at-gmail.com Name: Xijiang Pan
Organization: Tsinghua University, Beijing
Title-Subject: [Filtered] How to perform bake out on CM serios TEM
Question: I am wondering if there is anybody in this list farmiliar with the "bake out" procedure for the CM serious TEM. The local service engineers do not have any experiences in such work. So if you know,please contact me. Thanks in advance.
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Question: Hi All, Greetings for Merry X-mas and New Year.... Is there any general purpose image processing software that can do grain size analysis? I like to process AFM, SEM and TEM images eithr in their native format or as bitmaps.
Also, do you recommend any software for processing Selective area diffraction patterns? currently I write IDL code again, not very easy for students to use.
In both cases the software is intended to be used for use in teaching & research both. Price is an important concern
Daar coleagues I am working on a project on the vascular puncture inoculation (VPI) of maize viruses into maize embyos. We need to have a 3-D picture of the vascular bundles in (germinating) maize embyos, and a colleage of mine suggested MRI as a possible means to achieve that. I wonder if anyone has tried MRI on plants/ plant tissues, or if someone can suggest another more suitable method (apart from LM-serial sectioning). Thanks and Happy New Year to everyone.
----- Original Message ----- X-from: Joel Sheffield {jbs-at-temple.edu}
Hello,
I would humbly suggest Digital Volumetric Imaging (DVI), a technique which is being successfully used in our lab studying biomaterial implants.
The basic premise of the technique: it is automated serial sectioning and imaging of the block *face* of a fluorescently labeled EM-resin embedded sample. This allows excellent registration of the sections and subsequent 3D reconstruction in software.
Of course, it is a destructive technique and requires testing of fluorescent labels to ensure good contrast for your samples. But when optimized, the results are spectacular.
Have a look at the website of The Microscience Group:
http://www.microsciencegroup.com/
There are case studies and publications with sample images in the "Applications" section.
For your maize embryo, you could probably image at a field size of approximately 1.8mm^2 and resolution of 1.8um / voxel.
(Disclaimer: I am not affiliated with the company except as a satisfied user / customer)
-- Marc Takeno, Ph.D. Research Scientist Department of Bioengineering, University of Washington takenomm-at-u.washington.edu
---------- Forwarded message ----------
Hi The emerging maize embryo is about 1 mm thick and 3 mm long. Thanks for any suggestions.
----- Original Message ----- X-from: Joel Sheffield {jbs-at-temple.edu}
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Email: acruz04-at-cibnor.mx Name: Ariel Cruz
Organization: CIBNOR
Title-Subject: [Filtered] Problem EDX INCA 2000
Question: Some body know how can I do reinstall complete software for operation system. I need to intall again software for Hitachi S-3000N Oxford Inca 2000 complete procedure.
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Email: lamiller-at-uiuc.edu Name: Lou Ann Miller
Organization: Vet Med College, UIUC
Title-Subject: [Filtered] Forensic advice for a potential student
Question: Greetings,
I have a niece who found out she could graduate from high school early, but in the process has not had as much career guidance as she could have had in the school system.
She has an interest in doing the lab tests in the area of forensics, and I was wondering if any of you forensics folks out there would be willing to give me your email to pass onto her.
She would like to know how best to go about training for such a field, and what is all involved in the field.
(of course, being a Medical Technologist, I told her that would be a good base, but I think the actual background for a forensic scientist could be very different)
Thanks!
Lou Ann Miller
~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Lou Ann Miller, MT(ASCP) Service Supervisor Center for Microscopic Imaging College of Veterinary Medicine Rm 1204 VMBSB 2001 S Lincoln Ave Urbana, IL 61802
Dear All, thanks for the good discussion and the helpful tips to all on the list.
With the best wishes for a happy new year 2008, success at work and in business,
Stefan Diller
Please feel free to download and print out my new SEM Calendar 2008 (sorry, text only in German) with images from scanning electron microscopy: www.elektronenmikroskopie.info/Kalender2008_SD.pdf (29 MB). Images are Copyright Stefan Diller 2008, please ask for permission prior to any commercial use. Coming exhibition: "Die Ästhetik des Unsichtbaren" at the Botanical Garden Wuerzburg, February, 01st to February29th, 2008, opening at February, 01st 2008, 6 pm, see www.elektronenmikroskopie.info/ausstellungen/botanischer-garten-wuerzburg/Pl akat.pdf More infos at www.elektronenmikroskopie.info/ausstellungen/botanischer-garten-wuerzburg/ in some days.
This Question was submitted to Ask-A-Microscopist by (james99-at-uab.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, December 19, 2007 at 13:52:08 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both james99-at-uab.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: james99-at-uab.edu Name: James Borham
Organization: University of Alabama at Birmingham
Education: Undergraduate College
Location: Birmingham, Alabama, United States of America
Title: Finding a Specialized Microscope
Question: I'm trying to find a new microscope with some specialized features, and I'm having a lot of trouble. I'm hoping one of you may be able to help me out. The feature that is needed most, and has been impossible to find, is a non-stereo microscope and/or lens with a parfocalizing distance of 90 mm or more. Do you have any idea where I could find such a thing? Is a device with such a long parfocalizing distance even classified as a microscope? I would appreciate any help on this problem.
I did a quick Google search and found an advertizing press release for a stereo "inspection microscope" (sometimes referred to as a telescope) with a long (90mm) working distance. Some of the transmission electron microscopes also have binocs with similar properties to view the focusing screen. Sounds like this class of optics is what you want to locate. I know it is stereo, but just close one eye ;-). You might find a monocular inspection telescope somewhere. Here is the link for the article.
http://news.thomasnet.com/fullstory/20389
Cheers,
Dale Callaham
james99-at-uab.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question was submitted to Ask-A-Microscopist by (james99-at-uab.edu) } from } http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html } on Wednesday, December 19, 2007 at 13:52:08 } Remember to consider the Grade/Age of the student when considering the Question } --------------------------------------------------------------------------- } Please reply to both james99-at-uab.edu as well as to the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: james99-at-uab.edu } Name: James Borham } } Organization: University of Alabama at Birmingham } } Education: Undergraduate College } } Location: Birmingham, Alabama, United States of America } } Title: Finding a Specialized Microscope } } Question: I'm trying to find a new microscope with some specialized } features, and I'm having a lot of trouble. I'm hoping one of you may } be able to help me out. } The feature that is needed most, and has been impossible to find, is } a non-stereo microscope and/or lens with a parfocalizing distance of } 90 mm or more. } Do you have any idea where I could find such a thing? Is a device } with such a long parfocalizing distance even classified as a } microscope? } I would appreciate any help on this problem. } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 31 -- From zaluzec-at-microscopy.com Mon Dec 31 10:43:47 2007 } 8, 31 -- Received: from cubert.e-centre.net (morbo.e-centre.net [66.154.82.3]) } 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBVGhlg5000383 } 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 31 Dec 2007 10:43:47 -0600 } 8, 31 -- Received: from [10.3.1.19] (helo=barracuda2.stayonline.net) } 8, 31 -- by cubert.e-centre.net with esmtp (Exim 4.50) } 8, 31 -- id 1J9Njt-0003Pf-OC } 8, 31 -- for microscopy-at-microscopy.com; Mon, 31 Dec 2007 11:43:46 -0500 } 8, 31 -- X-ASG-Debug-ID: 1199119423-31474-157-0 } 8, 31 -- X-Barracuda-URL: http://10.3.1.19:8000/cgi-bin/mark.cgi } 8, 31 -- Received: from et-lax-21.site.stayonline.net (unknown [74.8.222.131]) } 8, 31 -- by barracuda2.stayonline.net (Spam Firewall) with ESMTP id 7472B198715F } 8, 31 -- for {microscopy-at-microscopy.com}