I am looking for microscopically orientated people who may be visiting South Africa in the next 6-12 months.
I have recently submitted and had accepted the DipRMS thesis, but am trying to assist the Royal Microscopical Society in finding a microscopist who would be able to convene a local viva board. The person would have to meet the Royal Microscopical Society's approval, as being a person able to perform such a function.
If you know of anyone or if you are able to assist yourself could you please contact me directly at
RETEP-at-ANAT.UCT.AC.ZA
or phone/write to me at the telephone number/address below.
PLEASE DO NOT respond via the Mailserver.
Peter D. G. Richards Dept Anatomy and Cell Biology UCT Medical School Observatory 7925 RSA Tel: 021-406 6285.
*************************************************************************** CALL FOR PAPERS ***************************************************************************
We are organizing a focused workshop entitled:
"INTERFACE FORMATION AND DYNAMICS IN LAYERED STRUTURES"
This workshop will be part of the Scanning Microscopy 1994 Meeting to be held in Toronto, Canada, 9-11 May, 1994.
The objective of this workshop is to bring together academic and industrial researchers working in the field of epitaxial heterostructures. Topics will include: -surface and interface energetics of defect formation -sensitivity of materials properties to interface imperfections -characterization techniques for interface studies -epitaxial growth modes -phase separation processes -point and line defect engineering -applications of mismatched materials.
The workshop has been organized on the same lines as the Gordon and European Science Foundation Research Conference format. Accordingly there will be much time for discussion. Moreover there will be a large number of invited speakers including:
G.C. Aers (NRC-Ottawa) J.-M. Baribeau (NRC-Ottawa) E. Bauer (U. Clausthal) D.K. Biegelsen (Xerox) D. Cherns (U. Bristol) A.G. Cullis (DRA-Malvern) C.B. Duke (Xerox) K. Eberl (Max Planck Inst.) L.C. Feldman (AT&T) E.A. Fitzgerald (AT&T) J.M. Gibson (U. Illinois) M. Grinfeld (Rutgers U.) D.E. Jesson (ORNL) B.A. Joyce (Imperial College) K.L. Kavanagh (UCSD) R.E. Mallard (BNR-Ottawa) B. Meyerson (IBM) B. Orr (U. Michigan) C.J. Palmstrom (Bellcore) H.E. Ruda (U. Toronto) M. Saran (Northern Telecom) L.J. Schowalter (Rensselaer) T. Tiedje (UBC) P.W. Voorhees (Northwestern U.) G.C. Weatherly (McMaster U.) Y.-N. Yang (U. Maryland) A. Zangwill (Georgia Tech)
The deadline for submission of contributed papers is 15 April, 1994.
For further information about the workshop please contact:
Doug D. Perovic Department of Metallurgy and Materials Science, University of Toronto, Toronto, M5S 1A4 Canada Tel: (416) 978-5635 Fax: (416) 978-4155 Email: perovic-at-ecf.utoronto.ca
I have always used gelatin capsules for embedding material in LR-White and I was wondering if anyone has tried using Beem capsules. If I dry the Beem capsules overnight in a 50 C vacuum oven, would this help? I'm currently doing immuno-labeling on Euplodes and would like to be able to spin the specimens down into a conical Beem capsule. Any suggestions? I appreciate any info on this method. Thanks, Phil Rutledge prutle1-at-gl.umbc.edu
This is a request for help. A zoology faculty member wants to count fiber types (muscle fiber cross sections, stained for I, IIA and IIB) and to obtain cross section areas for the fibers. If I understand the needs correctly, we will need software that will allow "sliding" two images and aligniment of the two for fiber type identification. Then, a more usual morphorometry of doing the X-sec. Defining the boundry by hand for the area determination is acceptable. Is there anyone who is doing this or has experience with muscle firber analysis that can recommend software for either the PC or Mac platform? I can capture the image into a PC system in my lab for them, save it in a standard format .tga or tiff etc., but have no microcomputer level analytical software. Any suggestions, vendors etc will be appreciated. Pardon the typo, live time VAX. Thanks David P. Campbell
We also ended up with a big mess when heat polymerizing LR White in polyethylene BEEM caps. However a polypropylene micro-fuge tube will work just fine. Only problem is that one must saw out the specimen or use doggie toenail clippers to cut off the end of the tube wherein lies the specimen and then tease it out. LR White does not bind to the polypropylene but it doesn't slip right out either. If you use the clippers be sure to do this inside your closed fist so that the specimen is not launched across the room never to be seen again. The clipped off tip must then be remounted on something appropriate for sectioning using super glue. Works best if you file done the cut surface so that it is smooth and makes good contact with the plastic stub such as those sold by Pella.
} Phil Rutledge asked about polymerizing LR-white in Beem capsules. We tried } drying at 60 C for 4 hours (haven't tried overnight). Results were } just as bad as with undried capsules. Poor polymerization of the resin. } We ended up with a real funky mess. } -Jay Jerome } jjerome-at-isnet.is.wfu.edu
I've never used LR-white, but I've talked with one of the distributors about its notorious polymerization properties. They told me that they think BEEM capsules are not suitable for polymerizing LR-white. The reason is that the capsules are too porous and permeable to water. Any water will interfere with polymerization. They have never had any trouble polymerizing, as long as they use gelatin capsules.
As I said, I have no experience (yet), but this might help others. I'd be interested in comments from those who use it successfully.
John chandler-at-lamar.ColoState.EDU Fort Collins, CO
Message-Id: {m0pbe6h-0000PNC-at-stjohns.ohsu.edu} Message-Version: 2 } To: microscopy-at-anlemc.msd.anl.gov
------------------------------------------------------------------------------ POSITION AVAILABLE ------------------------------------------------------------------------------
Research Associate postition open at the Department of Pathlogy in research related to cytoskeletal components of megakaryoscytes and the process of platelet fromation. Successful applicant will have a M.S. or equivalent, experience in electron microscopy and immunology techniques. Experience in standard biochemical and molecular biology techniques preferred.
Contact by phone or regular Mail:
Dr. Paula Stenberg, E.M. Director Department of Pathlogy, L113 Oregon Health Sciences University Portland, OR 97201
Message-Id: {m0pbeRw-0000P0C-at-stjohns.ohsu.edu} Message-Version: 2 } To: microscopy-at-anlemc.msd.anl.gov
Evan's Blue and Blood Brain Barrier
A general question for light level resolution. We use Evan's Blue circulated in vivo for 30 minutes to see if there has been a breakdown of the blood brain barrier. Animals are then perfused (4% para.) the brains sectioned with a vibratome (100 microns). Question is: If we dehydrate and mount in permount will the Evan's blue stay put or get leached out? Any experience with similar situation would be helpful.
Bob Kayton C.R.O.E.T. L606 Oregon Health Sciences Universtiy Portland, OR. 97007 503 494 2504 E-mail kayton-at-ohsu.edu
Message-ID: {MAILQUEUE-101.940302153004.576-at-anat.uct.ac.za} To: microscopy-at-anlemc.msd.anl.gov
Regarding the query about the azo vital dye Evans Blue.
I have not had any personal experiance with the dye but there should be no problem with dehydrating and mounting. Be sure to dehydrate in alcohols and xylene or equivelant.
Peter D. G. Richards Dept Anatomy and Cell Biology UCT Medical School Observatory 7925 RSA Tel: 021-406 6285.
Phil: our lab's experience with yeast, LR White and capsules: two ways: 1. small sample size: Place suspension of sample in LR White in a BEEM capsule, seal and centrifuge ( inside a tube, whose bottom is padded with cotton). Seal the BEEM INSIDE a large gelatin capsule. Available through drugstore or EM supply house. If you can't find a large enough gelatin capsule; use the OOO. Simply "trim" the beem capsule(that extra ridge where the cap goes on), so it will slide in.Then carefully seal the BEEM capsule inside two OOO gelatin capsule bottoms, that have been filled with LR White. A bit sloppy, but very effective. Works for us 100%. You just need to wear gloves and put down extra paper to catch any LR white drops, while you are filling and sealing 2. large sample size: leave material in OO capsules. Place these capsules inside a BEEM capsule, that has been cut to accomodate the capsules (The top half is cut away). Clinical centrifuge, as above.
hope this helps Louisa. Howard-at-dartmouth.edu
P.S. If you have access to a vacuum oven, you can use the BEEM capsules alone. This avoids the problems associated with porous BEEM capsules and the presence of oxygen.
Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: MICROSCOPY-at-ANLEMC.MSD.ANL.GOV
} Is there anyone who is doing this or has experience with muscle firber analysis } that can recommend software for either the PC or Mac platform?
NIH-Image, a freeware image processing program for the Macintosh available from the National Institutes of Health at zippy.nimh.nih.gov, directory /pub/image, has facilities for image alignment (in several modes), area determination (including thresholding/density slicing to segment the regions of interest, and automatic counting, analysis and labelling of these regions), and much more.
----- Transcript of session follows ----- 421 nw.oirtorm.net.kiae.su (tcpld)... Deferred: Connection timed out during user open with oirtorm.net.kiae.su
----- Unsent message follows ----- Received: from anlemc.msd.anl.gov by cpuv1.net.kiae.su with SMTP id AA11761 (5.65.kiae-1 for {alekseev-at-nw.oirtorm.net.kiae.su} ); Fri, 25 Feb 1994 02:43:27 +0300
In response to requests for suppliers of protective lacquer for electropolishing I would like to inform you that Microshield Lacquer is available from:
South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA
The Microshield Lacquer has been used for many years with our Model 550 Electropolisher and Model 550 Chemical Jet Polisher. It is acetone soluble and is available in an 8 ounce kit. Please reference P/N 02-01890-01.
} } Is there anyone who is doing this or has experience with muscle firber analysis } } that can recommend software for either the PC or Mac platform? } } NIH-Image, a freeware image processing program for the Macintosh } available from the National Institutes of Health at zippy.nimh.nih.gov, } directory /pub/image, has facilities for image alignment (in several } modes), area determination (including thresholding/density slicing to } segment the regions of interest, and automatic counting, analysis and } labelling of these regions), and much more.
Here's the README file from Image:
NIH Image --------- NIH Image is a public domain program for the Macintosh for doing digital image processing and analysis. It can acquire, display, edit, enhance, analyze, print, and animate grayscale and color images. It reads and writes TIFF, PICT, PICS and MacPaint files It features multiple windows, MacPaint-like editing and 8 levels of magnification. It supports Data Translation and Scion frame grabber cards. Image requires at least 4MB of RAM and 8-bit video. The following files in the directory /pub/image contain NIH Image, documentation, source code, and example images.
nih-image1xx_fpu.hqx NIH Image 1.xx application. Requires FPU. nih-image1xx_nonfpu.hqx Version that does not require floating-point nih-image1xx_docs.hqx Documentation in Word 5.0 format nih-image1xx_source.hqx Think Pascal 4.0 source images Directory with images in TIFF and PICT format stacks Example stacks(3D images and movies) (directory) nih-image_spinoffs Contains variants of NIH Image(directory) programs Directory containing miscellaneous related programs
File Formats ------------ Files are in one of three formats. Those with a ".hqx" suffix are BinHex encoded Mac binary files, those with a ".bin" suffix are MacBinary encoded Mac binary files, and those with a ".txt" suffix are a plain text files. The BinHex and MacBinary formats represent the two parts of a Mac file(the data fork and the resource fork) as a single file. They permit storage of a complete Mac file on a non-Mac system, such as this server.
Most files were compressed using the Mac utility Stuffit 1.5.1 and uploaded using Fetch, which does the BinHex or MacBinary encoding. Both utilities can be found in the util directory. The best way to retrieve files is to use Fetch, which automatically does Binhex (or MacBinary) decoding and file decompression. Unfortunately, Fetch requires a Mac directly connected to the Internet. If this is not the case, use an FTP (File Transfer Protocol) utility to transfer the files to a local host and then transfer them to a Mac via modem.
For Macs not directly on the Internet, BinHex files must be transferred to a Mac using "ascii" mode and then decoded and decompressed using Stuffit or some other Mac compression utility, such as Compact Pro. MacBinary files must be transferred to an intermediate host using FTP in "binary" mode, then transferred to a Mac in "MacBinary" mode and decompressed using Stuffit or Compact Pro. A copy of Stuffit 1.5.1 is in the directory /pub/util in MacBinary format. The document "ftp-primer.txt" in the documents directory provides more information on file formats and FTP.
NIH Image Mailing List ---------------------- There is an NIH Image mailing list. It was set up by a group in the Soil Science Department at the University of Minnesota. To subscribe, send a message containing the line "subscribe nih-image {your name} " to soils.umn.edu.
DepthGauge ---------- DepthGauge is a control panel that allows rapid switching between monitor depths settings(e.g. 8-bit color and 24-bit color).
Fetch(/pub/util) ---------------- Fetch is a slick utility that allows networked Macs to transfer files over the Internet using the File Transfer Protocol(FTP). It does BinHex decoding and file decompression as the files are transferred.
Giffer(/pub/image/peograms) ----------------------- Giffer is a shareware program useful for converting from GIF to Pict format, and vis-versa.
ImageFFT(/pub/image/image_spinoffs) ----------------------------------- ImageFFT is an extension to NIH Image to support frequency domain (power spectrum) display and editing. It can do a 512x512 FFT in 18 seconds on a Mac IIfx.
ImageFractal(/pub/image/image_spinoffs) --------------------------------------- ImageFractal is a version of Image modified to compute the Fractal Index of objects by the Richardson Plot or the Tile-amalgamation methods.
Image/MG(/pub/image/image_spinoffs) ----------------------------------- Image/MG is an extension of Image supporting quantitative evaluation of cerebral blood flow, glucose metabolism, and protein synthesis.
NCSA PalEdit(/pub/image/programs) ----------------------------- NCSA PalEdit is a public domain program from the National Center for Supercomputing Applications for creating and customizing color palettes. With PalEdit, you can modify the whole palette or individual entries in the palette, to create a set of colors tailored to your needs. PalEdit can save palettes in a format compatible with NIH Image.
MockWrite --------- MockWrite is a simple DA text editor that is convenient for editing macros written in Image's Pascal-like macro programming language.
Projectionist(/pub/image/programs) ------------------------------ Projectionist is a utility that allows you to animate stacks created by Image and saved in PICS format. Because all the frames in the stack do not need to be loaded into RAM, Projectionist requires less memory than image. This preliminary version uses the standard system palette, so stacks created with Image may lose some of their colors when animated with Projectionist.
There are a couple of solutions to this problem that we've used. First, capture one image, then print it on mylar with a laser printer (or to paper and photocopy onto mylar if your printer doesn't do mylar). then capture the image to be overlaid and hold the mylar transparency over the computer monitor. This usually works best with a high contrast image on the mylar- otherwise the gray in the background details will obscure the lookup image on the monitor. this works well for physical disectors. The second image can be grabbed during the long print time.
Second, NIH Image, a public domain image analysis program for the Mac, allows alignment of two overlaid images. You can capture the first image, duplicate it, and then capture the second image and perform a live paste which superimposes the live image over the captured image. Live paste allows moving the stage until the live image aligns. - This sounds easy, but usually quite tedious in practice.
The simplest way to align images in Image is to capture your images and then run one of the alignment macros that either come with Image or are avaialble from the User_macros folder from Zippy.nimh.nih.gove (NIH Image ftp source). One alignment macro allows drawing lines on images in a stack, then all members of the stack are automatically aligned. Another macro, which I wrote, works on pairs of images. Draw a line between two landmarks common to the two images, then the macro will rotate one of the images to rotationally align the two lines and transparently paste it over the other image. You tweak the XY registration by hand.
Most PC software with macro capabiblity should allow creating the same type of macros. 24 bit software may allow putting the two images to be aligned into different color planes, then aligning. - Glen MacDonald Hearing Development Laboratories RL-30 University of Washington Seattle, WA 98195 (206)543-8360 glenmac-at-u.washington.edu -
We just tried Evan's blue for the same purpose. It was somewhat visible in wet tissue coverslipped with glycerol. Dehydrating through ethanols and clearing in xylene, coverslipping with DPX (our standard protocol for paraffin and vibratome sections) dramatically improved the fluorescence. Nearly one week later there is no noticeable fading or diffusion. Sure is pretty.
Could you email your labelling method - concentrations, route of administration and survival times? I'll get the grad. student who did this to make her method available to send to you.
Glen MacDonald Hearing Development Laboratories RL-30 University of Washington Seattle, WA 98195 (206)543-8360 glenmac-at-u.washington.edu
I have read on this e-mail system about polymerization of LR WHITE RESIN in a vacuum oven. Do you close the lids of the BEEM capsules when in the oven or leave them open?
I`m interested in the morphology of Panthera sp. epidermis. If anyone knows something about it, please, contact me. Thank you.
============================================================================= Francisco Javier Hernandez Blazquez | Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-fox.cce.usp.br Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265 Departamento de Ciencias Basicas/Histologia| Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 611689 CEP 13630-000 Pirassununga (Sao Paulo) | BRAZIL | ==============================================================================
I`m interested in the morphology of Panthera sp. epidermis. If anyone knows something about it, please, contact me. Thank you.
============================================================================= Francisco Javier Hernandez Blazquez | Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-fox.cce.usp.br Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265 Departamento de Ciencias Basicas/Histologia| Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 611689 CEP 13630-000 Pirassununga (Sao Paulo) | BRAZIL | ==============================================================================
I have obtained fixed (2.5% glutaraldehyde in 0.1M sodium cacodylate buffer) lung (rabbit) tissue which has been embedded in LR White and Spurr resin. However, they have NOT been stained with osmium tetroxide prior to embedding. Could anyone suggest possible methods of osmium staining post sectioning? Thankyou.
Microscopists: I am interested in imaging from my TEM directly. For starters, let's say at 1Kx1Kx8bit. Is anyone out there putting the moral equivalent of a CCD in the column? (I suspect that you'd cook an actual CCD in a hurry, not to mention that you don't need response to photons). I see problems with file size, but what are the physical reasons that we don't capture images this way? Julian Smith III Dept. of Biology Winthrop University Rock Hill, SC 29733 Vox 803-323-2111 Fax 803-323-2246
subject:-TEM-PREPARATION OF TEM SAMPLES(PURE COPPER)
I would want to know if it is possible to indicate and to know the initial macro shear direction on a photo of a 3mm TEM disk sample submitted to an shear deformation by extrusion.Moreover, can we prepare and observe square samples instead of conventional disk samples for TEM.I know that some studies have been made in Russia using square samples and showing the direction of initial shear on the pictures. Thanks a lot by advance for any help. Stephane Ferrasse Email address:S0F6296-at-ZEUS.TAMU.EDU
I am studying pure copper samples with the Phillips EM400 and I have problems because my samples seem to be too thick to observe grains,subgrain boundaries and dislocations that I want to observe. My preparation is the following: 1-rough polishing with 600 grit abrasive powder to produce 100-150 micrometers thick parallel sided sheets by using a simple jig. 2-use of a conventional punch device to obtain a 3mm disk 3-use of the Tenupol automatic double jet electropolisher The electrolyte is:20% nitric acid-80% methanol (conditions:methanol cooled in dry ice). QUESTIONS: 1-I don't know if the voltage that I use is good(8-10V);the same for the value of my flow rate (it takes me about 2-4mn to thin my specimen under these conditions) 2-Is it better to have small (those I obtained are about 40-100 micrometer in diameter) or big holes for TEM observations ? 3-For observing grains and subgrains do we have to use diffraction patterns or Kikuchi lines (as it is the case for dislocation observations) to find a better orientation ? More generally, is orientation important to see grain boundaries? 4-I use the highest voltage (120 kV) .Is it correct? 5-Is there something wrong in my initial preparation ( electrolyte,..)? Thanks a lot by advance for any help, Yours Faithfully. Stephane Ferrasse University of Texas A&M phone:409-845-1790 (USA) Email address:S0F6296-at-ZEUS.TAMU.EDU
Message-ID: {MAILQUEUE-101.940307083235.256-at-anat.uct.ac.za} To: microscopy-at-anlemc.msd.anl.gov
I am passing on a query from Peter Linder (Linder-at-botany.uct.ac.za) regarding clearing agents for Botanical Specimens.
He is working with pollen and was wondering if anyone knew of a clearing agent(s) that dealt effectively with tannins. The ones he has used tend to make a mess of the tannins so that they form obnoxious lumps.
Any suggestions?Peter D. G. Richards Dept Anatomy and Cell Biology UCT Medical School Observatory 7925 RSA Tel: 021-406 6285.
Does anyone know the manufacturer of gelatin capsules or if a conical gelatin capsule is available? Since LR-White doesn't seem to work with BEEM capsules, has anyone tried UniCryl with BEEM capsules? I'm doing a lot of cell suspensions for immuno and a conical capsule is ideal. I appreciate all info on this subject. Thanks, Phil Rutledge prutle1-at-gl.umbc.edu
Sponsored by Japan Chapter of SPIE, Co-sponsored by BACUS and SPIE
22 April 1994 Kanagawa Science Park Kawasaki City, Kanagawa Japan ============================================================
Contents ========
1.0 Symposium on Photomask and X-Ray Mask Technology 2.0 Hotel Accommodations 3.0 Registration Information 4.0 General Information 5.0 How to Receive More Information
1.0 PHOTOMASK AND X-RAY MASK TECHNOLOGY =======================================
Symposium Chair: Touru Yoshizawa, SPIE Japan Chapter Steering Committee Chair: Yoshio Tanaka, Oki Electric Industry Co., Ltd. (Japan)
Steering Committee Members: Naoaki Aizaki, NEC Corp. (Japan); Hideaki Hamada, ETEC Systems Japan Ltd. (Japan); Naoya Hayashi, Dai Nippon Printing Co., Ltd. (Japan); M. Ohtaki, Toppan Printing Co., Ltd. (Japan); K. Nakashima, Lasertec Corp. (Japan); Norio Saitou, Hitachi, Ltd. (Japan); Kazumasa Shigematsu, Fujitsu Ltd. (Japan); Yoshiki Suzuki, KLA Japan Ltd.; Yoichi Takehana, HOYA Corp. (Japan); Tadahiro Takigawa, Toshiba Corp. (Japan); Yoshihiro Todokoro, Matsushita Electronics Corp. (Japan); Yaichirou Watakabe, Mitsubishi Electric Corp. (Japan); Hideo Yoshihara, NTT Advanced Technology Corp. (Japan); James A. Reynolds, Reynolds Consulting (U.S.)
2.0 HOTEL ACCOMMODATIONS ========================
The Japan Travel Bureau, Inc. (JTB) has been appointed as the official travel agent for the symposium and will handle reservations of hotel accommodations. Inquiries and applications should be addressed to:
Japan Travel Bureau, Inc. International Travel Division Convention Center (Ref. CD100748-014) 1-13-1 Nihombashi, Chuo-ku, Tokyo 103 Japan
Hotel KSP (Symposium site) 3-2-1 Sakado, Takatsu-ku Kawasaki, Kanagawa 213 Phone: +81-44-819-2211 Y12,650 single w/bath Y24,200 single use of twin room Y25,300 twin w/bath
Hotel Sunroute Den-En Takatsu (15-minute walk to the conference site) 508 Futako, Takatsu-ku Kawasaki, Kanagawa 213 Phone: +81-44-814-3122 Y8,500 single w/bath Y18,000 twin w/bath
Note:
1. The room rates do not include meals. 2. 3 or 6% tax and 10% service charge will be added to the bill when checking out. 3. Should you fail to arrive at the hotel on your scheduled check-in date, your reservation will be automatically canceled.
Application and Payment -----------------------
Participants wishing to reserve hotel accommodations should contact the JTB no later than 20 March 1994. Application for hotel accommodations should be accompanied by remittance of the hotel deposit (one-night room charge) plus a handling fee of Y500 and sent to JTB. No reservation will be confirmed in the absence of this payment. Personal checks are not accepted. All payments must be in Japanese yen. Payment should be in the form of:
* a bank transfer to the Japan Travel Bureau, Inc., account at the Bank of Tokyo, Marunouchi Office, 1-4-2 Marunouchi, Chiyoda-ku, Tokyo 100 Japan (Account number 1025740/Ref. CD100748-014)
* bank draft payable to the order of the Japan Travel Bureau, Inc.
* The following credit cards may be used for payment of hotel deposit: Master Card, Diners Club, Visa Card, AMEX.
Cancellation ------------
In the event of hotel reservations which must be canceled, written notification should be sent to JTB. The following cancellation fees will be deducted before refunding:
Up to 9 days before the first night of stay: Y2,000 Up to 2 to 8 days before: 20% of daily room charge (minimum Y2,000) Less than 2 days before, or no notice given: 100% of daily room charge
3.0 REGISTRATION INFORMATION ============================
To preregister for Photomask Japan '94 contact:
Secretariat-Photomask Japan '94 c/o Business Center for Academic Societies Japan Conference Department 5-16-9 Honkomagome, Bunkyo-ku, Tokyo 113 Japan
Phone: +81-3-5814-5800 Fax: +81-3-5814-5823
* Registration Fees:
SPIE Member . . . . . . . . . .Y25,500 Working Group Member (e.g., BACUS) . . . . . . . Y28,500 Nonmember . . . . . . . . . . .Y30,000
* Cancellation Policy:
No refunds will be made for cancellations received after 16 April 1994.
* Method of Payment (choose one):
All payment must be made in Japanese yen.
* A bank draft for total fee payable to the order of Photomask Japan.
* Bank transfer to the account of Photomask Japan, A/C No. 075-1834926, Daiichi Kangyo Bank, Hongo Branch, Tokyo.
* Credit card (Visa, Diners Club, MasterCard, or American Express)
On-site registration will be accepted at the conference site on 22 April. If you must register on site, please plan to arrive 30 minutes before the program begins. Registration badges are required for admittance to the conference. The technical conference will begin at 8.30.
4.0 GENERAL INFORMATION =======================
* Registration and Information Hours
Registration takes place in front of KSP Hall, Third Floor
Friday 22 April 8.00 to 16.00
* Speakers'/Chairs' Registration Desk
Located in front of KSP Hall, Third Floor
Friday 22 April 8.00 to 16.00
* Location/Travel Information
Photomask Japan '94 will be held at the Conference Hall of Kanagawa Science Park (KSP Hall), 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa, Japan. Phone: +81-44-819-2211
* Climate
The temperature in April ranges between 10 degrees C and 19 degrees C and the average humidity is 65%.
* Visa Requirements
Participants from countries which require a visa to enter Japan should apply at the nearest Japanese embassy well in advance of departure. If you have any questions or requests to obtain a visa, please contact the Secretariat-Photomask Japan ;94 as soon as possible.
Secretariat-Photomask Japan '94 c/o Business Center for Academic Societies Japan Conference Department 5-16-9 Honkomagome, Bunkyo-ku, Tokyo 113 Japan
Phone: +81-3-5814-5800 Fax: +81-3-5814-5823
5.0 HOW TO RECEIVE MORE INFORMATION ===================================
The complete text of the printed advance technical program for Photomask Japan '94 is available via anonymous FTP at:
It is also available through SPIE's automated e-mail server:
Send an e-mail message to,
info-optolink-request-at-mom.spie.org
with the following text in the message body:
send [optolink.meetings.programs]FILENAME.txt
To request a printed advance technical program via e-mail contact:
spie-at-mom.spie.org
For information regarding this meeting or other SPIE symposia or publications, contact SPIE at:
P.O. Box 10 Bellingham, WA 98227-0010 USA Telephone: 206/676-3290 (Pacific Time) Telefax: 206/647-1445 Telex: 46-7053 E-mail: spie-at-mom.spie.org Anonymous FTP: mom.spie.org.
This advance technical program is based on commitments received up to the time of publication and is subject to change without notice.
---------------------------------------------------------------- SPIE is a nonprofit society dedicated to advancing engineering and scientific applications of optical, electro-optical, and optoelectronic instrumentation, systems and technology. Its members are scientists, engineers, and users interested in the reduction to practice of these technologies. SPIE provides the means for communicating new developments and applications to the scientific, engineering, and user communities through its publications, symposia, and short courses.
SPIE is dedicated to bringing you quality electronic media and online services.
********************************************************************** Biological X-ray microanalysis - applications and recent developments **********************************************************************
The biological XRMA group of the Royal Microscopical Society are holding their Spring Meeting at the University of Sheffield on April 7th 1994. Papers will be presented on a variety of subjects including specimen preparation, elemental mapping, analysis of plant tissue extracts and biological-material interactions. Registration fees are 25 pounds for RMS members and 35 pounds for others.
Further details are available from:
Dr Paul Hatton, School of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield S10 2TA, UK
Sender: kayton-at-ohsu.edu (Robert Kayton) Message-Version: 2 } To: microscopy-at-anlemc.msd.anl.gov
We just tried Evan's blue for the same purpose. It was somewhat visible in wet tissue coverslipped with glycerol. Dehydrating through ethanols and clearing in xylene, coverslipping with DPX (our standard protocol for paraffin and vibratome sections) dramatically improved the fluorescence. Nearly one week later there is no noticeable fading or diffusion. Sure is pretty.
Could you email your labelling method - concentrations, route of administration and survival times? I'll get the grad. student who did this to make her method available to send to you.
Glen MacDonald Hearing Development Laboratories RL-30 University of Washington Seattle, WA 98195 (206)543-8360 glenmac-at-u.washington.edu
There is an immediate opening for a Scanning Electron Microscopy (SEM) Technician at Exxon Research and Engineering Company's Corporate Research Laboratory in Clinton, New Jersey. As a member of the Microcharacterization Team at Corporate Research, this position will involve the operation of a JEOL SEM with associated Energy Dispersive and Wavelength Dispersive Spectrometers. The position will involve the creative application of high resolution SEM imaging and elemental characterization of samples related to a wide range of projects at Exxon's Corporate Research Laboratory. The position will also involve general laboratory operations, sample preparation, SEM maintenance and computer analysis of the SEM data (both image analysis and spectral analysis).
Successful candidates should have experience in chemistry, physics or material science with a Baccalaureate degree or equivalent experience. Previous SEM experience and experience with high vacuum systems and computers (DOS, Unix, and VMS) is highly desirable. Since a number of projects are simultaneously in progress, it is essential for the SEM technician to be very organized and to pay attention to detail and accuracy in reporting results.
Qualified candidates please send resume to:
William Lamberti Associate Research Physicist Exxon Research and Engineering Company Clinton Township Route 22 East Annandale, New Jersey 08801
Equal Opportunity Employer M/F/H/V
All resumes must be received by April 8, 1994.
William A. Lamberti Office LA-196 Exxon Research and Engineering Company Route 22 East Annandale, NJ 08801 Email: walambe-at-erenj.com
TEM-PREPARATION OF PURE COPPER SAMPLES Thank you first to all those who have given me useful information about preparation of pure copper samples. I would want to know however something else about specimen washing and more precisely about storage of copper.I have read (Eddington-Practical electron microscopy) that Cu can not be stored because of oxydation.Then must we use Cu immediatly after jet polishing or can we keep it in a dessicator?If it is the case, for how long? Thanks in advance for any help. Regards. Stephane Ferrasse University of Texas A&M--email:S0F6296&ZEUS.TAMU.EDU
I realize that the Microscopy Listserv is predominantly used for those microscopies involving light or electrons, however, for those interested in "ions" this may be of interest!
(page 1)
- FIRST NOTICE -
SEVENTH ANNUAL WORKSHOP ON SECONDARY ION MASS SPECTROMETRY
Microelectronics Center of North Carolina (MCNC) and Holiday Inn - RTP
Research Triangle Park, NC
May 10-12, 1994
This workshop is an informal gathering of scientists for discussions relating to fundamental aspects, instrumentation and applications of Secondary Ion Mass Spectrometry.
PROGRAM
On Tuesday evening, May 10, 1994, the Seventh Annual Workshop on SIMS will commence with a registration/mixer from 7 - 9 pm. On Wednesday morning, May 11, a special topics session will focus on fundamentals, application and techniques related to secondary ion imaging. Other topics of interest will be accepted for inclusion in follow-on sessions or the third day's program. Following Wednesday's sessions there will be an evening cocktail hour, dinner, and vendor presentations. The third day will be devoted to user's group meetings and contributed presentations.
The format for this workshop will be informal, with topics of interest to both the novice and experienced SIMS user. You are encouraged to prepare an oral or poster presentation on any aspect of SIMS that may be of interest to your fellow workshop attendees. Open forum user's groups are planned for magnetic sector, time-of-flight, and quadrupole-based mass spectrometer instruments. Technical representatives from the instrumentation companies have been invited to address issues involving instrumentation, modifications, methodologies, and new equipment.
______________________ (page 2)
PRESENTATIONS Please indicate on the registration form provided if you are interested in presenting an oral or poster presentation on a topic which may be of interest to other workshop attendees. An abstract must be submitted to the organizing committee by April 15, 1994. As a special option this year, you are invited to submit a full length manuscript for a special issue of the new journal - Microbeam Analysis .
REGISTRATION Please note: the registration fee is $50.00 if received before April 25, 1994; a late registration fee of $100 will apply after this date. The SIMS Workshop is now being held under the auspices of the Microbeam Analysis Society. Checks should be made payable to: Microbeam Analysis Society and sent directly to Dr. Steven Hues with the completed registration form below - to be received no later than April 25, 1994. Note: The registration fee is waved for full-time student presenters only!
LOCAL ACCOMMODATIONS A block of rooms has been reserved at the Holiday Inn - Research Triangle Park, NC [919-941-6000], at a reduced rate of $72/night + tax (mention the SIMS Workshop). Rooms must be reserved before April 19, 1994 to be eligible for this reduced rate. All inquires pertaining to reserving rooms or details on the facilities, as well as payment for your room, should be made directly to the Holiday Inn - Research Triangle Park, NC. The closest airport with free Holiday Inn Shuttle connection (~10 min.) is the Raleigh-Durham International. Bus transportation to and from the hotel and MCNC is also provided.
____ Yes, I will submit a manuscript for publication in a special issue of Microbeam Analysis.
_______________________ (page 3)
ORGANIZING COMMITTEE:
Steven M. Hues Naval Research Laboratory (202) 767-2671
Greg Gillen National Institute of Standards and Technology (301) 975-2190
Richard T. Lareau Army Research Laboratory (908) 532-0119
IMPORTANT: Registration form and the $50 registration fee must be received no later than April 25, 1994 !!!
Please complete the registration form provided (see pg. 2), cut at dotted line, and return with registration fee enclosed (made payable to the Microbeam Analysis Society) to:
Steven M. Hues Naval Research Laboratory Code 6170 4555 Overlook Ave., S.W. Washington, D. C. 20375-5342
____________
Best regards,
Richard T. Lareau, Ph.D. US Army Research Laboratory Electronics and Power Sources Directorate AMSRL-EP-EC-M, Myer Research Center Fort Monmouth, New Jersey 07703-5601
We are considering the purchase of a stage heater/cooler for the Nikon Diaphot. Our main concern is cooling, e.g. stable tempertaure at approx. 22 degrees C. Does anybody have a recommendation of a particularly good stage temperature controller. Right now we have a water cooled system but problems include 1.) vibration and 2.) too narrow for access with high N.A. objectives. Thanks- Michael Cammer
Message-Id: {MAILQUEUE-101.940310095650.288-at-redhot.hut.fi} To: MICROSCOPY-at-ANLEMC.MSD.ANL.GOV
The European Microbeam Analysis Society (EMAS) organises
The 1st EMAS Regional Workshop on June 15-17, 1994, in Helsinki, Finland:
Electron probe microanalysis of materials today - practical aspects
The workshop is intended as a comprehensive introduction for those who are involved with SEM+EDS or EPMA based microanalysis applied to inorganic materials. The emphasis will be on the possibilities and limitations of the methods, and to give enough guidelines, procedures and examples in order to keep the discussion, for which there will be ample time, on a practical and basic level. The participants are encouraged to bring in their own problems for debate. The workshop language is English.
Main subjects:
* Electron-specimen interaction: X-ray generation & detection, spatial resolution * Analytical electron microscopy (AEM): possibilities and limitations * Correction procedures in microanalysis * Practical problems with EDS analysis & practical aspects of WDS analysis * Characterisation of EDS detectors * Standardless vs. quantitative EDS analysis: what can you expect? * SEM + EDS or EPMA with WDS? * Thin surface layer (1 nm - 1 m) analysis with EPMA or SEM + EDS * AEM as a tool for practical problems * How accurate are your results? * Strategy for applying microanalytical techniques
The main lecturers are: Dr. Peter Karduck (Gemeinschaftslabor fuer Elektronenmikroskopie, RWTH Aachen), Dr. W. A. Patrick Nicholson, Dept. of Physics & Astronomy, Univ. of Glasgow) and Dr. Peter Willich (Fraunhofer Institut fuer Schicht- und Oberflaechentechnik, Hamburg).
Posters are invited. Interpretation of the word "regional" can be done in a broad sense. For additional information please see below.
Erkki Heikinheimo ************************************************************* Erkki Heikinheimo e-mail: eheikin-at-redhot.hut.fi Helsinki Univ. of Technology Lab. of Metallurgy SF-02150 Espoo phone +358-0-4512759 FINLAND fax +358-0-4512798 *************************************************************
Has anyone had any experience in processing bacteria grown on 3mm glass beads for SEM? The cells are grown in suspension and the positioning of the beads is irrelevant. Want to look at surface for adhesion properties study. I want to try normal SEM processing procedures but would appreciate any info on an easy method. Thanks, Phil Rutledge prutle1-at-gl.umbc.edu
We are working on an immunoEM project localizing different epitopes in S. mansoni worms. Some of the life cycle stages are fairly small. We are embedding in Unicryl but we have some problems with the small specimens. I tried to put them in gelatine but this gives a problem with the sectioning properties of Unicryl. Does anyone have a better solution. We thought of putting them in agar, but I don't like the temperature this requires (immunoreactivity).
Greetings, We use LOW gelling temperature agar for our tiny specimens. Sigma sells a variety of these, and we use type VII. I forget exactly what the melting T is, but it melts readily on the low setting of a hot plate. We fix, rinse, and then put a few microliters (5-15?) of molten agar around the sample. It sets up "instantly". We use a 2% agar/water solution. Then we dehydrate and embedd as usual. We also throw in a bit of fast green at 100% ethanol, for ease of finding the samples. I have taken this stuff into several kinds of resin and never had any prboblems sectioning, light or em level. Also, we routinely do immunocytochem localizing microtubules, a fairly senstive antigen, so I don't the heat is a big problem. I hope this is useful for you. Please reply if you have further q's. Good Luck, Tobias Baskin
} Hi, } } We are working on an immunoEM project localizing different epitopes in S. } mansoni worms. Some of the life cycle stages are fairly small. We are } embedding in Unicryl but we have some problems with the small specimens. } I tried to put them in gelatine but this gives a problem with the } sectioning properties of Unicryl. Does anyone have a better solution. We } thought of putting them in agar, but I don't like the temperature this } requires (immunoreactivity). } } Any help would be appreciated. } } -------------------------------------------------------------------- } Universitaire Instelling Antwerpen (UIA) } Lab Pathology } 2610 Wilrijk } BELGIUM } } Tel: 32.3.820.25.34 } Fax: 32.3.820.22.48 } E-mail: anapat-at-uia.ac.be } ------------------------------------------------------------------- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Tobias I. Baskin Baskin-at-biosci.mbp.missouri.edu University of Missouri * Division of Biological Sciences * 109 Tucker Hall Columbia, MO 65211 USA voice: 314-882-0173 fax: 314-882-0123 ___ ____ ^ ____ _____ / \ / / \ / \ / / | / / \ / / /___ / /__ /_____\ / /__ / / / \ ( / / / / \ \ / / /____ / \ \____/ /_____
Does anyone know a reference with a derivation of the brightness equation relating brightness to accelerating voltage? Shimoyama, etal, 1972, seem to pull it from the air, and then everyone else seems to just reference them. Shimoyama has B=IeV/pikT where I is emission current density, V is relativistic voltage, T is filament temp. This is stated to be the relativistically corrected expression of the Langmuir theoretical value. Langmuir's equation is I=I(0) (eV/kT) sin2(phi) where I is the max current density in a focused spot, I(0) is the current density at the cathode, T is temp, and phi is the half angle of focused spot.
So does anyone know of a derivation of the brightness vs voltage equation that can be believed??
Subject: Time:11:24 AM OFFICE MEMO Bright vs kV Date:3/11/94 Roseanne: I think you can find the matter of the variation of brightness and accelerating voltage treated in a very understandable manner in Hall's 2nd Ed of "Introd. to Electron Micros.". One of the best general explanations of the characteristics of self-biased guns with tungsten filaments is contained in Chs. VI and VIII of the book "The Electron Microscope" by M. E. Haine, InterSci Pubs. I ran off some calcs of the variation of brightness vs both temp and kV for an illustration in class one time - if you send me your FAX number, I'll be glad to send you a copy. BIGELOW-at-UMICH.EDU FAX: 313-763-4788
Subject: Time:11:37 AM OFFICE MEMO Add'n on Bright. vs kV Date:3/11/94 Sorry, I neglected to mention that Hall's discussion of brightness vs kV appears on p. 158.
I don't do EM, and I've never worked with worms, but I have embedded small pieces of rat spinal cord in agar for vibratome sectioning and it didn't hurt immunoreactivity any for a number of peptides, 5-HT, or tyrosine hydroxylase. The agar stays molten at about 50-55 ¡C, and your tissue is exposed to that for only a very short time. It may even help to cool (not freeze!) your samples slightly before embedding - just a thought. All I did was to attach the blocked pieces to a glass coverslip with SuperGlue, and then squirt the molten agar over and around them. It really isn't embedding in the sense that I'm sure the agar doesn't permeate the tissue at all, but it gave sufficient support to allow sectioning in a consistent plane. You might also consider Low Melting Point agarose (I believe it's available from BRL?) which would keep temperature to a minimum, but it's expensive. Hope this helps...
David Morilak Dept Psychiatry Stanford University morilak-at-cmgm.stanford.edu
RE: Getting a small hole while electropolishing Another "trick"
Another method of setting the automatice cutoff for light sensor s in electropolishers is to temporarily replace your specimen with a conventional TEM aperture. Use an aperture with about a 10-20 micron size hole (it can be an old used one out of your scope) and then turn on the entire electropolishing unit (except the voltage) get the flow of liquid running and adjust the "sensitivity" until your instrument just detects the hole. This is a bit more systematic than just arbitrarily setting the sensor without a calibration point.
You could try embedding in alginate. This can be done at room temperature (or cooler). We have used a method based on Tamponnet et al, Stain Technology 1988, v 63, 155-158. for ultrastructure of free cells and protoplasts.
Mix the cells with a 1-2% sodium alginate solution in buffer (0.1M cacodylate in the original). Extrude the solution through a narrow pippet/syringe into a solution containing 50mM Calcium chloride (or let drops fall into this, or spread a thin film on a slide and dip into this) where the alginate coagulates and holds the cells together.
The only problem we have had is with different batchs of alginate. Some coagulate well and some fail to.
} Do you have } reason to think that the epidermis in the cat family is different in some } special way from the epidermis of other mammals?
I wish thank you for your answer. I will give some explanation. I was in my lab when a engineer asked to speak to me. He was very excited about something he had discovered about the way that the epithelial cells of Panthera pardus epidermis are disposed. He wanted some information or a slide (histological section) of this animal to confirm his ideas, but he did not explained what ideas or what was his intention. He alleged professional secret. That sounds strange, isn t? I am a public employee so I must aid people who ask me aid, they pay my salary. I did a search in Biological Abstract and found nothing. I will do a search in Zoological Abstract, maybe I will be luckier. Thank you.
============================================================================= Francisco Javier Hernandez Blazquez | Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-fox.cce.usp.br Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265 Departamento de Ciencias Basicas/Histologia| Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 611689 CEP 13630-000 Pirassununga (Sao Paulo) | BRAZIL | ==============================================================================
I need to convert TIFF files generated in a PC program using specification 5 to TIFF files following specification 6 for use on a Silicon Graphics machine that requires specification 6. Does anyone know of a program that can do this conversion? Thanks for any pointers.
-- ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Nancy L Desmond, Ph.D. Department of Neurosurgery University of Virginia Health Sciences Center, Box 420 Charlottesville, VA 22908
Message-Id: {MAILQUEUE-101.940314085545.384-at-parmly1.parmly.luc.edu} To: microscopy-at-anlemc.msd.anl.gov
} From: rutledge phil {prutle1-at-gl.umbc.edu}
} Has anyone had any experience in processing bacteria grown on 3mm glass } beads for SEM? The cells are grown in suspension and the positioning of } the beads is irrelevant. Want to look at surface for adhesion properties } study. I want to try normal SEM processing procedures but would } appreciate any info on an easy method. } Thanks, } Phil Rutledge } prutle1-at-gl.umbc.edu } Probably the quickest way is to pour your bacteria/beads solution into a funnel with a 0.22 or 0.45 micron nucleur-pore membrane filter, then run your fixatives/ OsO4/ dehydration etc. solutions through the same funnel, maybe 5 min. each by volume sample. Dry by CPD or hexamethyldisilizane at 60 C or Peldri. Pour out onto stub with something sticky & conductive on it, like conductive tape or a THIN coat partially dried silver paste (not paint). Phil Oshel
} Do you have } reason to think that the epidermis in the cat family is different in some } special way from the epidermis of other mammals?
I wish thank you for your answer. I will give some explanation. I was in my lab when a engineer asked to speak to me. He was very excited about something he had discovered about the way that the epithelial cells of Panthera pardus epidermis are disposed. He wanted some information or a slide (histological section) of this animal to confirm his ideas, but he did not explained what ideas or what was his intention. He alleged professional secret. That sounds strange, isn t? I am a public employee so I must aid people who ask me aid, they pay my salary. I did a search in Biological Abstract and found nothing. I will do a search in Zoological Abstract, maybe I will be luckier. Thank you.
============================================================================= Francisco Javier Hernandez Blazquez | Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-fox.cce.usp.br Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265 Departamento de Ciencias Basicas/Histologia| Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 611689 CEP 13630-000 Pirassununga (Sao Paulo) | BRAZIL | ==============================================================================
Sharath Yes you did miss something. Look in the EMMPDL subdirectory called CBED. There is a program there by John Porter which plots Stereograhic projections &CBED patterns. By adjusting the convergence angle you will get a conventional SAED pattern. I'm not sure if the UMICH library mirror has the master copy, however I would be very suprized if it doesnot since John Mansfield is very careful on backups and copies. I've appended a copy of the program abstract to this message.
Nestor Zaluzec ANLEMC --------------- Title :STERPROJ_IBM_V4 Keywords :Stereographic Projection, CBED, SAD, TEM, AEM, HOLZ Computer :IBM PC/XT/AT or compatible Operating System :PC-DOS Programming Language :QUICKBASIC 4.5 Hardware Requirements :Hewlett Packard HP7470A Plotter required. Author(s) :John R. Porter Correspondence Address :Rockwell International Science Center, :Thousand Oaks, CA 91360. Abstract:
This QuickBASIC program plots stereographic projections, CBED and HOLZ line simulations, and performs axis/angle pair calculations. Output is to the screen or a Hewlett Packard HP 7470A Plotter. Stereographic projections are drawn to scale for subsequent manipulations with a standard 18cm. Wulff net and can be plotted for cubic, hexagonal, tetragonal and monoclinic crystal systems in any orientation. Plotted poles can be restricted to those allowed by structure factor considerations (for certain structures) or restricted to those in certain Laue zones. The program leads the user through menus to determine the structure and orientation for the projection, which is then plotted accordingly. Version 4.00 is significantly enhanced compared to earlier versions.
EDITORS NOTE: THIS IS A NEW UPDATED VERSION OF STERPROJ- IBM (FEB.1990) -----------------------------------------------------------------------------
Subject: Time:5:16 PM OFFICE MEMO Bright. & Acc. Voltg. Date:3/14/94 My previous comment in response to Roseann Csencsits did not did not actually answer her question concerning the origin of the equation commonly used for brightness vs acc. voltage, because Hall doesn't take into account relativistic effects. A better source is the book "Transmission Electron Microscopy" by Reimer (Springer-Verlag 1984). The underlying equation is Eq. 4.10 on p. 90, which Reimer derives in a fairly understandable manner. This equation can be recast in terms of accelerating voltage U (in Reimer's terminology) by substituting eU for E and mc^2 for Eo (see table 2.1, p. 21), whereupon the terms for the 'relativistic voltage' Ur= U + (e/2mc^2)U^2 can be formed, and the equation becomes b = j/pi + (j/pi)(e/kT)Ur. Assuming the (j/pi) term is small compared to the rest of the equation, and can be neglected, this reduces to the approximation Roseann asked about. For a good definition of the 'relativistic voltage' see p. 30 of Spence. The experimental measurement of brightness is discussed by Spence in Sect. 7.2. Reimer also discusses this the brightness equation in Sect. 2.1 of his book "Scanning Electron Microscopy".
This is my second attempt to get some feedback on my request to get off the microscopy list. I've also sent the proper (but nonfunctional) message to listserver. Still I am swamped with msgs from the list.
Please remove my name from the list, and please let me know if there's a problem. I'm not the only person with this problem - there are enoguh of us that we are considering forming a new list: those_who_can't_unsubscribe_from_the _microscopy_list :}
This is my second attempt to get some feedback on my request to get off the microscopy list. I've also sent the proper (but nonfunctional) message to listserver. Still I am deluged with msgs from the list.
Please remove my name from the list, and please let me know if there's a problem. I'm not the only person with this problem - there are enoguh of us that we are considering forming a new list: those_who_can't_unsubscribe_from_the _microscopy_list :}
To: WALCKSD:ml:wpafb, SCHELTFJ:ml:wpafb Subj: Thinning Cerium Oxide Orig-Author: {del-at-sol1.lrsm.upenn.edu (David E. Luzzi)}:ddn:wpafb ----------------------------------------------------------- We need to thin a single crystal of Cerium Oxide (CeO2) to electron transparency but are having major difficulties with brittleness. Does anyone have experience with this, or analogous materials? Thanks in advance.
David E. Luzzi Dept. of Materials Science University of Pennsylvania 3231 Walnut Street Philadelphia, PA 19104-6272
I am looking for two second-hand reflective optical microscopes. One should be something like Bausch&Lomb Stereo Zoom 7, the other should have well calibrated focal depth. Can anyone tell me where to look? Thanks.
Lumin Wang TEM Lab., Univ. of New Mexico Bitnet: zircon-at-unmb
Lumin Wang wrote: } I am looking for two second-hand reflective optical microscopes. One should } something like Bausch&Lomb Stereo Zoom 7, the other should have well } calibrated focal depth...
I have no information on used microscopes. Maybe a dealer could help. I am, however, involved in importing new microscopes of all types. In fact, we have a 10X-160X zoom that is priced very low.
There have been a few problems during the last fe months with users trying to unsubscribe from the list. For the most part the problems were related to how they tried to unsubscribe, the MOST common problem was related to mail forwarding! The basic problem was that the particuliar user would subscribe from one address then have that computer forward mail to a different second address on a differnet computer system.
Upon attempting to Unsubscribe, the user would supply his/her second address to the server, rather than the original subscription address. Since the "second" address was unknown to the server nothing happened and they continued to receive their mail! This has happend at least a dozen times in the last few months so please remember to Unsubscribe you must inform the server of your original subscription address, not the one to which you have your mail forwarded. This is particuliarly important for sites/users that heavily use aliases and forwarding, so try to give me a break and remember from whence you came!.
The other problem which requires less investigative work, but is still a headache is that you must unsubscribe to the Listserver not to Microscopy List. (See below)
Also I've had a few comments come back saying that people are not sure if their messages have been posted. If your message is posted you WILL receive a copy back of your original message. The most common problem here is some subscribers simply "REPLY" to a message. This is fine AS LONG AS your Email system recognizes that the message came from the MICROSCOPY-at-ANLEMC.MSD.ANL.GOV, then there is no problem Some Email systems reply to the originator of the message (i.e. the person who started the question/comment). If this is the case then the members of this listserver will not see your reply! Please check your Email header before you send out a REPLY and insure that the message is going to MICROSCOPY-at-ANLEMC.MSD.ANL.GOV. If you do not receive a copy of the message you posted, then it is 99+% likely that your message was not posted to the subscription list....
Just a reminder to Unsubscribe you should send a message to:
Listserver-at-anlemc.msd.anl.gov
and include the following line in your mail message
Unsubscribe Microscopy Username-at-HostAddress
where Username = User Name that you originally subscribed with HostAddress = Address of the host you originally subscribed from ====================================================================
Alot of traffic goes through the computer now adays, and I'm pretty much swamped with things to do.... Yes, the new Alpha system has finally arrived, however, haven't had time to setup the software so it will still be awhile before anything is upgraded. We are now approaching the 900 subscriber mark so business is booming, but the Gov. has cut back our funds & people.
The never-ending-battle continues.........
Nestor J. Zaluzec ANL EM Center & Proprietor of the "Hotel California" Microscopy Listserver :-)
On the question of brightness vs voltage, I REPLYed, which sent my response only to the sender. Others may be interested in Principles of Electron Optics by P.W. Hawkes and E. Kasper, Academic Press, a good two-volume set with more than I want to remember about electron microscopy.
The 46th Annual Meeting of the Scandinavian Society for Electron Microscopy, June 13-15, 1994, Kuopio, Finland
INVITATION AND CALL FOR CONTRIBUTIONS
SCIENTIFIC PROGRAM
The aim of the symposium is to focus interest broadly in the recent developments of microscopy, not only emphasizing the importance of electron microscopy but paying attention also to the development of e.g. confocal microscopy, immunocytochemistry, image processing and image analysis.
The scientic program is designed to have two plenary sessions and parallel sessions for biological and materials scientists. The following sessions are included in the program: - High resolution microscopy - Confocal microscopy - Image processing, analysis and quantitation (plenary) - Electron microscopy in molecular biology - Scanning probe microscopies (plenary) - Electron beam assisted microanalysis - Immuno electron microscopy - Environmental cell pathology of plants - Electron microscopy in clinical medicine - New techniques and products
Invited speakers are amongst others A. Bardal (Norway), C.-H. von Bonsdorff (Finland), F. Cuisinier (France), S. Enestršm (Sweden), H.E. Gaub (Germany), H. Gundersen (Denmark), M.S. Gunthardt-Goerg (Switzerland), T. Holopainen (Finland), S. Huttunen (Finland), L. Kanerva (Finland), I. Kottke (Germany), T. Lepistš (Finland), A.B. Maunsbach (Denmark), M. Mšrgelin (Sweden), J. Paranko (Finland), J.C. Russ (USA), M. Ruhle (Germany), E. Soini (Finland), P. Willich (Germany).
LANGUAGE
The congress language is English
ABSTRACTS
Papers are invited on all aspects of electron microscopy and related techniques. The extended abstracts will be published as a separate proceedings book of the meeting. The abstracts, including photos and references, may contain two pages. The first page should contain text only, the second page may be used for text, figures and tables. The abstract must neve exceed two pages. Use a word-processor with TIMES 12 font and italics for taxonomic terms. The text and figures must fit inside a rectangle measuring 160 mm x 240 mm (width x height). One camera-ready original with one photocopy should be sent before March 31, 1994 to: Dr. Raija Tammi, Department of Anatomy, University of Kuopio, P.O.Box 1627, FI-70211, Kuopio, Finland
POSTERS
At the poster exhibition, a short time will be reserved to each participant for oral presentation. An area of 1100 x 1300 mm (width x height) is allocated for the poster.
SOCIAL PROGRAM
A Get-Together Party will take place on June 12, at the Snellmania Building of the University of Kuopio. On June 13, we take off for a cruise on Lake Kallavesi and continue with the smoke sauna and supper at JatkŠn- kŠmppŠ (Lumberjacks' Lodgings). On June 14, there is reception at the City Hall and after this the conference dinner at the Hotel Arctia
REGISTRATION
The registration fee is 800 FIM for members, 950 FIM for non-members (if you join the society you will get advantage of membreship immediately), and 650 FIM for students and technicians. The fee includes the Get- Together Party, daily lunches and coffees and one copy of the published abstracts. Please contact SCANDEM 94 Secretariat, c/o Finnish Medical Society Duodecim, Savilahdentie 6, FI-70210 Kuopio, Finland (fax. Int.+ 358-71-240361). Deadline is April 15, 1994. Surcharge for registration is 150 FIM.
DEADLINES
March 31, 1994 Submission of abstracts April 15, 1994 Registration
LOCATION
Kuopio is located in the centre of the Lake-district of Finland about 400 km northeast of Helsinki. Connections to Kuopio by air, train or bus are good. The venue site of the meeting is the Snellmania Building of the University of Kuopio close to the centre of Kuopio.
David, I also routinely make TEM samples from GaAs and InP, which are reasonably brittle (but not as bad as YBCO). I simply mount the samples in thermoplastic wax on a glass slide about 20 mm x 20mm and polish down to about 100 microns using the finest grit paper I can find - 'worn out' 1200 grit paper is fine. I then mount it in Lacomit on a PTFE or nylon stub and chemically polish it to transparency using Cl in methanol jet from a wash bottle chopped in half and held upside down in a clamp. I can make 30 samples in a morning and the kit costs virtually nothing to make! The only thing to be careful of is to make sure that the wax ('Crystalbond' in my case) lies all around the sample so that no corners stick out, and to go slowly. If your material is as bad as 1-2-3 superconductor, you may need to be a bit more high-tech; I haven't done it myself, but work done here used a Dimpler to very slowly polish down to about 5 microns before ion milling to transparency. The samples took about 2 days to make and when they broke (about 1 in 5 samples) it was heart rending. I can give you the Email address of the person who did this, if you want - he's moved on now.
Regards,
Richard Beanland,
Department of Materials Science and Engineering, University of Liverpool, P.O. Box 147, Liverpool L69, 3BX, England.
} From: Francisco Javier H Blazquez {fjhblazq-at-fox.cce.usp.br} } } Do you have } } reason to think that the epidermis in the cat family is different in some } } special way from the epidermis of other mammals? } Try doing a literature search of "Science" and "Nature" for the last 5 or so years, using "self-organizing" and "Turing" in your keywords along with "leopard" & "jaguar". The title may have been something along the lines of "How the Leopard got his spots". Also, it may have been in the research news section and not an article. There was a short piece (which I copied & can't find), about some group discovering that the spots on a leopard's (or jaguar's) where a self-organizing phenomenon similar to some chemical reactions (the ones that swirl in a petri-dish?); the principle involved had been proposed orginally by Alan Turing (of math & comupter fame). Your engineer may have stumbled across this. ? Then again, it may be more mundane, like something to do with intercellular junctions. Phil Oshel (312) 274-6348
I just subscribed, got my welcome to microscopy notice and then sent out 2 messages regarding LM on the same day (about a week ago). I never saw either one or heard any response - although I have been catching up on the EM discussion of thinning CeO, etc. Just checking did my messages get out? Is there somewhere I can browse old messages from the list - an ftp site?
P. Joyce
Peter Joyce GRA Materials Science, University of Texas Phone: (512) 471-5723
I have two questions. We have a Leica MeF3 inverted metallograph we just purchased about a year ago. The bulb in the main lamphouse burned out and it was not easy to replace. The manual calls it a 12V/100W LV halogen bulb. When removed all it says on it is Philips. I tried to get Leica to tell me what it's called and who to get it from apart from them (they only had one in stock in Houston) and they were no help at all - although they did try to help me, sort of. The supplier they gave me as their source didn't know anything about being an OEM soure for Leica or Reichert or whoever they are??? What I'm looking for is anyone who has been this or as similar cycle with Leica equipment, or the name of a GOOD specialty bulb store that's been tried and tested on microscope equipment. I tried one really poor one in FLA and another not so bad in NY with little success.
Second question is related to an earlier post of mine from a few months back. I inquired about the use of index matching fluid to help observe my specimens. The response raised more questios than answers, thanks just the same. Experiments have shown that witha thin layer of oil and no coverslip (coverslip helps keep my optics safe from contamination, esp. inverted scope!) the difference in my specimens is night and day. I get really good clarity. The facts are - I'm looking at rough composite prepreg (carbon fibers in polysulfone) and the surface is much too diffuse to see anything. I went to our Plant Biology dept. who gave samples of permount oil, Zeiss immersion oil, mineral oil, and clove oil and some coverslips. The coverslips had little or no effect on optical performance with the oils. Also the Zeiss immersion oil was not very fruitful. The other 3 oils, in particular the clove oil gave excellent clarity at about 200x in darkfield. I went back to the Plant Biology guys for data on the clove oil, specifically the refractive index - they couldn't help me. I called the chemical supplier Sigma Chemical they haven't done that test. I checked the library, there no such data on clove oil and no mention at all of permount oil or mineral oil in the Chemistry CRC and the like. Is there anyone out there using the immersion technique who can comment on the use of such oils and the source of such data? Anyone ever studied clove oil? We're also observing a strange side effect, when the oil is applied tiny white lines, (maybe cracks in the surface of the material) become visible. We're not sure if they're there before the application of the oil because before the oil is applied we can't see well enough. The available data wouldn't suggest this type of oil should be attacking the polysulfone. Perhaps this effect actually arises from the oil getting in pre-existing cracks and highlighting them. Any comments.
P. Joyce
Peter Joyce GRA Materials Science, University of Texas Phone: (512) 471-5723
} I have two questions. We have a Leica MeF3 inverted metallograph we just } purchased about a year ago. The bulb in the main lamphouse burned out and } it was not easy to replace. The manual calls it a 12V/100W LV halogen } bulb. When removed all it says on it is Philips. I tried to get Leica to } tell me what it's called and who to get it from apart from them (they only } had one in stock in Houston) and they were no help at all - although they } did try to help me, sort of. The supplier they gave me as their source } didn't know anything about being an OEM soure for Leica or Reichert or } whoever they are??? What I'm looking for is anyone who has been this or as } similar cycle with Leica equipment, or the name of a GOOD specialty bulb } store that's been tried and tested on microscope equipment. I tried one } really poor one in FLA and another not so bad in NY with little success.
I just happen to have my local independent light microscope service person in the lab today doing routine maintenance. He tells me that, unless you're using a specialty housing, you should do fine with any standard 12V 100W lamp of the ANSI code FCR. You can get these from OSRAM, Philips or Ushio. There should not be any real difference between them. This lamp is rated at 50 hours lamp life at 12V.
John chandler-at-lamar.ColoState.EDU Fort Collins, CO
There were a couple of questions concerning LM bulbs and where to purchase them. I quit going to the manufacturers of LMs unless there was no alternative. I have been buying my bulbs from Bulbman,Inc. in Reno, Nevada for over 10 years. They seem to be pretty knowledgable about the bulbs and they have always been able to provide the bulbs or tell me where they may be purchased other than the manufacturer. Their toll free number is 1-800-648-1163. They also take PO's for small amounts of bulbs. You don't have to order more than you need to get a good price if the bulb you want is available. Hope this info helps. Phil Rutledge
I am seeking contact addresses/faxes/e-mail for distributors and manufacturers of CCD video cameras for biological light microscopy (currently brightfield, darkfield, phase and DIC) to be used in conjunction with a VCR and Macintosh Quadra 650 and image grabbing and manipulating facilities.
A colleague in this department uses a Pulnix TM-765 for similar purposes and this camera would be ideal. I have faxed the President of Motion Analysis, Inc at Eugene, OR, which was the the agent when he purchased his camera a couple of years back, but have had no reply. Can anyone give me other contacts or agents for Pulnix, and/or information on other manufacturers/agents for similar systems. We need a camera control system also, and hoped to pay only in the $2500-3000 range.
Any information on Australian distributors would also be welcome.
Thanks
Nikki Watson
Dr N.A. Watson Department of Zoology University of New England Armidale, NSW, 2351 AUSTRALIA Fax: 067 711 869 Phone: 067 732181
For additional support you can cut the conical tip from a BEEM capsule, place your sample insidek (i did this for rat spinal cord) and fill the capsule with agar. Then take the cap off, supergl;ue the thing to the slide after trimming down the open end of the BEEM capsule to expose a couple of millimeters of sample.
a thin walled polyethylened vial cap also works, if you can find an appropriate size, and will be easier to retirm if you need to keep sectioning farther down.
Glen MacDonald Hearing Development Laboratories RL-30 University of Washington Seattle, WA 98195 (206)543-8360 glenmac-at-u.washington.edu
On Fri, 11 Mar 1994, David Morilak wrote:
} I don't do EM, and I've never worked with worms, but I have embedded small } pieces of rat spinal cord in agar for vibratome sectioning and it didn't hurt } immunoreactivity any for a number of peptides, 5-HT, or tyrosine hydroxylase. } The agar stays molten at about 50-55 !C, and your tissue is exposed to that } for only a very short time. It may even help to cool (not freeze!) your } samples slightly before embedding - just a thought. All I did was to attach } the blocked pieces to a glass coverslip with SuperGlue, and then squirt the } molten agar over and around them. It really isn't embedding in the sense that } I'm sure the agar doesn't permeate the tissue at all, but it gave sufficient } support to allow sectioning in a consistent plane. You might also consider Low } Melting Point agarose (I believe it's available from BRL?) which would keep } temperature to a minimum, but it's expensive. Hope this helps... } } David Morilak } Dept Psychiatry } Stanford University } morilak-at-cmgm.stanford.edu } } ------- }
I would suggest buying refractive oils from Cargille Labs in Cedar Grove New Jersey, 201-239-6633. They sell liquids certified for refractive index from about 1.25 to 2.0 (this is from memory, and therefore mostly suspect). I have used refractive index kits containing 80 or so liquids that cover a range, but if you are just looking for something for immersion microscope objectives that is more consistant than clove oil they can supply one quarter ounce bottles.
} I am seeking contact addresses/faxes/e-mail for distributors and } manufacturers of CCD video cameras for biological light microscopy } (currently brightfield, darkfield, phase and DIC) to be used in conjunction } with a VCR and Macintosh Quadra 650 and image grabbing and manipulating } facilities. } } A colleague in this department uses a Pulnix TM-765 for similar purposes } and this camera would be ideal. I have faxed the President of Motion } Analysis, Inc at Eugene, OR, which was the the agent when he purchased his } camera a couple of years back, but have had no reply. Can anyone give me } other contacts or agents for Pulnix, and/or information on other } manufacturers/agents for similar systems. We need a camera control system } also, and hoped to pay only in the $2500-3000 range. } } Any information on Australian distributors would also be welcome. } } Thanks } } Nikki Watson } } Dr N.A. Watson } Department of Zoology } University of New England } Armidale, NSW, 2351 } AUSTRALIA } Fax: 067 711 869 } Phone: 067 732181 } } (using 'Eudora' on a Macintosh)
I have looked around in my files and have a few suggestions and corrections.
In my lab we use a couple of Hamamatsu C2400 CCD-video cameras, one with an intensifier coupled to it. I could not find the price for just the CCD camera + controller, but you can contact
Robert A. Wick, Ph.D. Photonic Microscopy, Inc. 2625 Butterfield Road, 103 West Oakbrook, IL 60521 Tel 312-571-1241 Fax 312-571-1244
Other addresses+numbers (form Laser Focus World, Buyers' Guide, 1994)
PULNIX America, Inc. 1330 Orleans Dr. Sunnyvale, CA 94089
Tel 408-747-0300 Fax 408-747-0880 ___________________________________
Dage-MTI Inc 701 N. Roeske Ave. Michigan City, IN 46360
Tel 219-872-5514 Fax 219-872-5559 ___________________________________
I am studying pure copper and copper-niobium samples with SEM.I would want to observe microstructural morphology of the specimen.Is some specific preparation of the specimen needed (for example special cleaning,etching...)?I would want to avoid any kind of preparation involving high temperatures. Thanks in advance for any help. Stephane Ferrasse Texas A&M University_E-mail:S0F6296&ZEUS.TAMU.EDU
A laboratory in our department is seeking a buyer/barterer for an Axiomat with good Nomarski (the machine was used for microtubule motility assays etc.) and other optics. If you are interested, please send us a message. Thanks- Michael Cammer cammer-at-aecom.yu.edu
Greetings, Has anyone tried dehydration in acetonitrile instead of ethanol, as suggested by Edwards et al Micr. Res. Tech 21:39-50 (1992)??? Experience with plant tissue would be particularly relevant, but any comments will be welcomed.
In Canada we have a vendor that sells Sony CCD video cameras, the address is: Soquelec, 5757 Cavendish blvd, suite 101 Montreal, Quebec, Canada, H4W 2W8 Tel:514 482-6427 fax:514 482 1929
Hoping that it will be of some help. Diane Montpetit Food Research Center, Agriculture Canada Quebec, Canada.
In Canada we have a vendor selling Sony CCD cameras: Soquelec 5757 Cavendish blvd, suite 101, Montreal, Quebec, Canada, H4W 2W8 tel:514-482-6427 fax:514-482-1929 Hoping that it will be of some help Diane Montpetit Food Research Center, Agriculture Canada, Quebec, Canada
Introduction to the structure of computer networks Local networks and network protocols; Larger networks; The Internet; Network structure at Tulane Medical Center
Local network functions Controlling network connections with the Chooser; File sharing; E-mail; Networked scheduling; Lab system access; Remote access (connecting from home); Software tools: Alias Manager; Microsoft Mail; Eudora; Meeting Maker; Termy; Apple Remote Access; ARA Commander.
Using the Internet IP addresses and MacTCP; E-mail and internet addresses; Listserv groups; Telnet and File Transfer Protocol (FTP); Transfer formats: binhexing and compression; Special communication protocols: the World Wide Web and Gopher; USENET news groups; Internet resources in the biomedical sciences; Software tools: MacTCP, NCSA Telnet, Fetch, Versaterm Link, Binhex 4.0, Stuffit, Compactor, Mosaic, TurboGopher, ph.
Reference searching and management Using Grateful Med for Medline searching; Current Contents on diskette; Reference management with EndNote; Software tools: Grateful Med 2.0, EndNote Plus, Microsoft Word
Procedures for connecting to the network at Tulane
In Canada we have a vendor selling Sony CCd cameras: Soquelec 5757 CAVENDISH BLVD, SUITE 101, MONTREAL, QUEBEC, CANADA, H4W 2W8 TEL:514 482-6427 FAX:514 482-1929
HOPING THAT IT WILL BE SOME HELP,, dIANE MONTPETIT, FOOD RESEARCH CENTER, QUEBEC, CANADA ,MONTPETITD-at-QCRSSH.AGR.CA
I am looking for suggestions on thin sectioning polymeric materials (polypropylene/synthetic rubber) for TEM analysis. I am having difficulty transfering sections (~80 nm) from the knife to grids. Common problems with the sections are curling and flying away. I was wondering if anyone has had any success embedding these types of materials. *************************************** David Garrett "DGARRETT-at-GAB.UNT.EDU" University of North Texas Dept. Biological Sciences (817)565-3964 Fax (817)565-4136 ***************************************
Plastic sectioning I am looking for suggestions on thin sectioning polymeric materials (polypropylene/synthetic rubber) for TEM analysis. I am having difficulty transfering sections (~80 nm) from the knife to grids. Common problems with the sections are curling and flying away. I was wondering if anyone has had any success embedding these types of materials. *************************************** David Garrett "DGARRETT-at-GAB.UNT.EDU" University of North Texas Dept. Biological Sciences (817)565-3964 Fax (817)565-4136 ***************************************
If you are sectioning on a dry knife you might like to try the trick that Tokuyasu used for recovering cryosections. Pick them up on the surface of a drop of sucrose or other inert solution held in a small wire loop. The liquid will stop your problems with static and the surface tension should flatten out the sections. Once the sections are recovered, place the liquid drop onto a coated grid. The recovery solution can be removed by floating the grid specimen face down on drops of water. I have seen this method used successfully for rubberized samples in Arizona. It will not be of much use to you if you cannot wet your sample.
Message in response to questions asked by Stephane Ferrasse, Texas A&M.
I assume you are interested in the morphology and distribution of niobium precipitates within a copper matrix. I have worked on rapidly solidified Cu-Nb microcomposites at Iowa State a couple of years ago and have published a technique which has given me excellent results in the SEM. Detailed procedures may be found in ASTM STP 1165, Metallography: Past, Present and Future, George Vander Voort et al., ASTM, 1993, article written by myself and collegues from Iowa State and Penn State.
Briefly, the technique involves an attack-polish method to remove some of the cumatrix. After a standard grind polish finishing on 0.25um diamond with kerosene as the lubricant, the sample was first immersed in a 20%HF, 20%H2SO4, 20%HNO3, 40%H2O solution to remove the smeared Nb created during polishing. Then the samples are immersed in a 30%H3PO4, 15%CH3COOH, 10%HNO3, 45% Ethanol solution to remove some of the copper surrounding the Nb ppts. To aide in this process, this step was performed in an ultrasonic cleaner - promoted homogeneous removal of copper. Final rinsing was done in the ultrasonic cleaner using fresh, 111 trichlorotrifluoroethane. The copper removal and final cleaningstep was repeated as necessary until the desired results were obtained.
Kevin L. Zeik U.S.Steel Technical Center Monroeville, PA 15146 EMail_Zeik-at-USS.COM
To: MICROSCO:CENTRAL cc: DRStadden:R_D:Armstrong Subj: Image Archiving Forwarded: Message from DRStadden:R_D:Armstrong of 3-23-94 ------------------------------------------------------------------ SEE FOLLOWING
--------------------- Forwarded Message Body --------------------- To: MICROSCO:CENTRAL cc: DRStadden:R_D:Armstrong Subj: Image Archiving ------------------------------------------------------------------ I'm curious about whether anyone is routinely electronically archiving images from SEM or LM, and/or sending them over a PC network. If so, what sort of hardware is required; particularly, what is retrofittable to a digital imaging SEM or a PLM, and what does it cost? I have one quotation for an SEM-interfaceable unit that is around $20K.
Thanks for any leads,
Dave Stadden Testing and Analysis Lab Armstrong World Industries, Inc.
When grabbing the image with CCD camera I a often getting problem with the very high signal which when looking at preview is almost white. I use f=22 but the signal is still to strong. The only solution besides a pice of hardware is to deminish the light source but it is a bad solution. Is there any software for Mac which can solve my problem ?
Michel Deschuyteneer suggested CD-ROM as a method for archiving images. This is something we have been trying to do for the last few months. If you plan to purchase a CD writer, you need to be aware that you may also need to buy a high speed hard disk from which the images will be downloaded to the CD. Look carefully at the input specifications of the CD writer before you buy the high speed disk.
Russell E. Cook Electron Microscopy Center for Materials Research Materials Science Division Argonne National Laboratory Argonne, Illinois 60439 USA
Russell E. Cook Electron Microscopy Center for Materials Research Materials Science Division Argonne National Laboratory Argonne, Illinois 60439 USA
------------------ RFC822 Header Follows ------------------ Received: by qmgate.anl.gov with SMTP;25 Mar 1994 09:41:29 U Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
Ron Wroblewski asked about software for the Mac to help his intensity saturation problem....
The simple answer is you need a hardware fix not a software one. If the CCD is saturating then there is no software solution. You must decrease the signal. Some CCD camera's have autoIris lenses which attempt to adjust the input to the CCD by closing down the iris via a motorized mechanism.. Alternatively you could put in some type of neutral density filter to reduce the signal that is hitting your CCD.
Yes, we have just (today!) purchased the above printer for producing STEM/XRAY images primarily. For effectiveness/cost ratio it is nothing short of superb. But it does have some limitations: can be SLOW for continuous tone color, does not support Postscript (yet - they "tell me" that a 3rd. party is working on this), fonts can be a problem but not if you store them as image files when used as text on micrographs, line drawings can get excessive cases of the "jaggies" from the mechanical drive ( only shows up on images if you magnify } 5X or so. There is a very objective review of this printer in the August 1993 edition of "Advanced Imaging" Vol 8 No 8 pp 37 - 39. P.S. If you use a Mac you'll need to purchase a $199 interface.
Peter Ingram ingram-at-rcc.rti.org =============================== 3)
Some time ago we had a demo of the Fargo Primera (wax transfer) printer and compared it to the Tektronix Phaser IIID which we have on site. The system demoed only had a three colour system so the blacks were not great. A 3 colour + black system is available. The ouput was not quite as good as the Tek. but when you consider that the Fargo was around a quarter of the price (in Australia) it was pretty good value. I have seen the dye sub. output but not had a chance to do direct comparisions.
I think that Byte reviewd the printer not long ago (or it may have been another computer journal).
I hope this is of some use.
Colin V.
John Fournelle Electron Microprobe Lab Internet:johnf-at-geology.wisc.edu Dept of Geology & Geophysics Telephone: (608) 262-7964 University of Wisconsin Fax: (608) 262-0693 1215 West Dayton Street Amateur radio: WA3BTA/9 Madison, WI 53706 (14.030, 21.030 mHz)
Journal of Microscopy, Vol. 174, Pt. 1, April 1994, pp. 1 - 14.
STANDARD PERFORMANCE CRITERIA FOR ANALYTICAL ELECTRON MICROSCOPY
by S. M. ZEMYAN & D. B. WILLIAMS , Department of Materials Science & Engineering, Lehigh University, Whitaker Laboratory, . 5 E. Packer Ave., Bethlehem, PA 18015, U.S.A.
Summary Users of analytical electron microscopy lack easy-to-use standards for assessing the consistency and quality of analytical performance. We propose using a Cr thin film of known thickness to measure three important characteristics related to performance: the Cr K alpha peak-to-background (P/B) ratio, the X-ray spectrometer relative efficiency, and the spectrometer energy resolution. We used a Cr specimen to determine the instrumental factors which influence the P/B ratio, finding that the highest P/B ratios are achieved in scanning transmission mode at the highest available accelerating voltage. We present values of the P/B ratio, and the detector relative efficiency and energy resolution which can be used for comparison in other laboratories using the standard film.
Journal of Microscopy, Vol. 174, Pt. 1, April 1994, pp. 15 - 22.
ATOMIC FORCE MICROSCOPY IN THE PHOTOCHEMISTRY OF CHALCONES
by G. KAUPP , Universitaet Oldenburg, FB 9 - Organische Chemie I, Postfach 2503, D-26111 Oldenburg, Germany
Summary The application of atomic force microscopy (AFM) to photodimerization of crystalline chalcones provides new insights into the detailed mechanisms of solid-state reactions on the molecular level. Well-directed long-range transport phenomena are found which reach far beyond the crystal lattice distances. Reactions occur in the surface region where the light is absorbed. Characteristic features are built up that depend on crystal structure and crystal face. This could not be foreseen by previous theories based solely on a topochemical postulate/principle. There is now a much more intimate correlation of crystal structure with solid-state reactivity and this is directly studied and proven experimentally by AFM. Even solid-state reactions which are in opposition to topochemistry can be studied and understood on a molecular basis. The three-dimensional resolution of undisturbed insulating surfaces which is obtained by AFM is not available by any other technique.
Journal of Microscopy, Vol. 174, Pt. 1, April 1994, pp. 23 - 30.
REGRESSION METHODS FOR AUTOMATED COLOUR IMAGE CLASSIFICATION AND THRESHOLDING
by E. J. BREEN , CSIRO, Division of Mathematics and Statistics, Institute of Information Science and Engineering, Locked Bag 17, North Ryde NSW 2113 Australia
Summary Regression methods are used to perform automated image thresholding and colour pixel classification. This is done by considering threshold levels and pixel classification labels as pattern attributes. A regression equation that performs a mapping from the J dimensional feature-pattern space to the K dimensional attribute space is derived. The approach is non-parametric and deterministic, hence no assumptions about the statistical properties of the input patterns or images need be made. Initially a known set of input patterns with associated attributes are used to constitute a training set. A mapping function is then determined from the training patterns and used for estimating attribute values from unknown input patterns, such as images.
Journal of Microscopy, Vol. 174, Pt. 1, April 1994, pp. 31 - 38.
LIP-MODEL-BASED THREE-DIMENSIONAL RECONSTRUCTION AND VISUALIZATION OF HIV-INFECTED ENTIRE CELLS
by P. GREMILLET,* M. JOURLIN + & J.-C. PINOLI ++ , *Misis Image, BP 711, 42950 Saint-Etienne Cedex 9, France. + Laboratoire Lyonnais des Signaux et Systemes, Ecole Superieure de Chimie, Physique et Electronique, 31 Place Bellecour, 69288 Lyon Cedex 02, France, and Laboratoire de Traitement du Signal, Universite de Saint-Etienne, 23 Rue Paul Michelon, 42023 Saint-Etienne Cedex 02, France. ++Pechiney, Centre de Recherches, BP 27, 38340 Voreppe, France
Summary This paper presents a global solution from acquisition to visualization for the three-dimensional reconstruction of cell sections. Original techniques are proposed for the correct handling of the geometrical section distortions, and a new interpretation based on the logarithmic image processing (LIP) model is applied in order to create normalized grey-level sections where these are missing. Finally, a new method for generating a mesh of triangles to describe the envelope of the reconstructed cell is proposed, as well as a visualization mixing image synthesis and grey-level information. The product allows the user to explore the reconstructed cellular block in any desired direction, by showing user-defined grey-level sections inside the block mixed to a synthetic view of the cell envelope.
Journal of Microscopy, Vol. 174, Pt. 1, April 1994, pp. 39 - 46.
SURFACE ULTRASTRUCTURE OF OLFACTORY RECEPTOR SENSE HAIRS IN THE SILKMOTH, ANTHERAEA PERNYI
by V. I. POPOV, A. A. NIKONOV, N. K. AGAFONOVA & E. E. FESENKO , Institute of Cell Biophysics, Russian Accademy of Sciences, Pushchino, Moscow Region, 142292, Russia
Summary The fine structure of silkmoth sense hair surfaces has been investigated by freeze etching with Pt/C rotary shadowing. To do this, the hydrophobic layer in the cuticle was removed using 20 - 25% methanol or ethanol. Freeze-etch patterns of sense hair surfaces, as well as the pore structure and pore distribution, are shown. The hair surface has a nonhelical `band' structure, in which every `band' lies at an oblique angle with respect to the axis of the hair. Each `band' is separated from its neighbour by a 30-nm step. The average density of pores is 11.3 plus or minus 2.4 pores per square micrometre. Freeze-etch patterns of the single and multiple pore tubules are shown. Evidence for direct contact between the pore tubule and dendrite membrane of an olfactory receptor neuron is presented.
Journal of Microscopy, Vol. 174, Pt. 1, April 1994, pp. 47 - 50.
AGAR STANDARDS FOR QUANTITATIVE X-RAY MICROANALYSIS OF RESIN-EMBEDDED PLANT TISSUES
by E. FRITZ & G. JENTSCHKE , Institute of Forest Botany, University of Gottingen, Busgenweg 2, 37077 Gottingen, Germany
Summary Calibration standards for quantitative X-ray microanalysis of resin-embedded plant tissue were prepared by adding 6 - 600 mm KC1 to 5% agar. Agar blocks with an edge length of 1 - 2 mm were rapidly frozen, freeze-dried and embedded in styrene-methacrylate. Dry sections 1 micrometre thick were mounted on adhesive-coated grids. Apart from fine-scale inhomogeneities caused by ice crystal formation, the KC1 is evenly distributed in the agar blocks. The peak-to-continuum values of K and Cl were highly linearly correlated to the K and Cl contents over the whole concentration range.
Journal of Microscopy, Vol. 174, Pt. 1, April 1994, pp. 51 - 54.
A SIMPLE METHOD FOR PROCESSING INDIVIDUAL OOCYTES AND EMBRYOS FOR ELECTRON MICROSCOPY
by C. NOGUES, M. MARTI, M. BOADA & M. PONSA , Departament Biologia Cel.lular i Fisiologia, Facultat Ciencies, Universitat Autonoma de Barcelona, 08193-Bellaterra (Barcelona), Spain
Summary A simple method for handling individual specimens that must be processed either for scanning or transmission electron microscopy studies is described. For scanning microscope processing, dehydration is carried out with samples enclosed in small cages made from TAAB capsules in which top and bottom are substituted by plankton nets, and for transmission electron microscopy, samples are pre-embedded in agarose. This procedure significantly reduces mouth pipetting, dissecting microscope observations, is less labour intensive and, most importantly, reduces sample loss.
Journal of Microscopy, Vol. 174, Pt. 1, April 1994, pp. 55 - 58.
ENHANCED RETENTION OF MAGNETIC PARTICLES (E.G. MICROTOMED SECTIONS) IN A TEM
by W. A. FURDANOWICZ & K. E. DOWNEY , Homer Research Labs, Bethlehem Steel Corporation, Bethlehem, PA 18016-7699, U.S.A.
Summary An electron-transparent layer of adhesive is used to restrain magnetic particles from being stripped off the support film by the magnetic field of an objective lens in a TEM.
ÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄ RAPID PUBLICATION Journal of Microscopy, Vol. 174, Pt. 1, April 1994, pp. RP1 - RP 2.
MONOMERIC COLLAGEN IMAGED BY CRYOGENIC FORCE MICROSCOPY
by M. B. SHATTUCK, M. G. L. GUSTAFSSON, K. A. FISHER, K. C. YANAGIMOTO, A. VEIS, R. S. BHATNAGAR & J. CLARKE
IF YOU ARE INTERESTED IN SUBMITTING A MANUSCRIPT TO THE JOURNAL OF MICROSCOPY, OR HAVE ANY OTHER QUESTIONS ABOUT THE JOURNAL, PLEASE CONTACT DR GILLIAN WILSON, JOURNAL OF MICROSCOPY EDITORIAL OFFICE, 37/38 ST CLEMENTS, OXFORD, OX4 1AJ, UNITED KINGDOM. TELEPHONE +44 865 248768, FAX +44 865 791237, EMAIL RMS-at-VAX.OX.AC.UK.
AA Happy EA H ÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄ
A belated reply to the person asking about cat skin. In felis domesticus the skin has all of the same layers as found in human skin. However, the skin is thinner because there are only a few layers of dermins (2-4 layers) as compared to the {10 layers found in most areas of human skin.
We also have been considering CD-ROM for archival and image transfer. I have been told that only recently some of the authoring drives allow reading a partially filled platter, and then only on the authoring drive, not on a standard reading-only drive. I was hoping to use CD-ROM as an alternative to buying magneto-optical drives for portable data, as oppposed to setting up a server.
What has anyone experienced or read about this?
Glen MacDonald Hearing Development Laboratories RL-30 University of Washington Seattle, WA 98195 (206)543-8360 glenmac-at-u.washington.edu
On Fri, 25 Mar 1994, Russell E. Cook wrote:
} Michel Deschuyteneer suggested CD-ROM as a method for archiving images. } This is something we have been trying to do for the last few months. If } you plan to purchase a CD writer, you need to be aware that you may also } need to buy a high speed hard disk from which the images will be downloaded } to the CD. Look carefully at the input specifications of the CD writer } before you buy the high speed disk. } } } Russell E. Cook } Electron Microscopy Center for Materials Research } Materials Science Division } Argonne National Laboratory } Argonne, Illinois 60439 } USA } } }
If all people are talking about is archiving, then I would strongly suggest that DAT tapes are considered. The prices of the hardware for CD-ROM and DAT are comparable, but the price of a single tape is $16 and it can hold 2-16 Gbytes of data. The only reason in my mind for using CD-ROM is if you want additional disc space which is less expensive than true hard disks; for archiving it may well be a waste of money.
As Robin Wright says, CDs are forever. Also, they allow random access and it is extremely cheap to buy an internal CD-ROM player with new computers
Glen
On Mon, 28 Mar 1994, L. D. Marks wrote:
} If all people are talking about is archiving, then I } would strongly suggest that DAT tapes are considered. The } prices of the hardware for CD-ROM and DAT are comparable, } but the price of a single tape is $16 and it can hold } 2-16 Gbytes of data. The only reason in my mind for using } CD-ROM is if you want additional disc space which is less } expensive than true hard disks; for archiving it may well } be a waste of money. } } Laurie Marks, NU }
Message-Id: {MAILQUEUE-101.940329082400.480-at-FS-IAM-1.JRC.NL} To: microscopy-at-anlemc.msd.anl.gov
I agree that DAT tape is not ideal for archiving due to eventual degradation etc, but what about these new Phase-Change optical drives? I don't know much about them, except that they are re-writeable, cost about half that of CD-ROM writers (at least in Europe) and can store approx. 1Gb. I think that they are sold by Panasonic over here. Maybe somebody else knows more about them. As far as I can see they appear to have significant advantages, and are based on Sony's mini-disk technology, so the prices should drop fairly soon if that is a success.
Doug Arrell Mechanical Performance and Joining Institute for Advanced Materials 1755 ZG Petten Netherlands
Subject: Time:8:16 AM OFFICE MEMO CD archiving Date:3/29/94 Just my two cents worth on archiving on optical discs, CDs or DAT. We have been looking into CD archiving and feel that it has several advantages over the other two formats. 1) CDs allow relatively fast and easy acess to the images/files. So if you are planning to manipulate these images (of course you will have to work on copys placed on your hard disk) or require frequent acess to them this method is about equal to opticals, but superior to DAT, 2) Both CDs & opticals will have a longer expected life then DAT before media breakdown, and 3) the most important consideration for us is that CD readers are cheap, going down in price and are incorporated into the hardware of an increasing number of computer systems. This is important since every potential user is already equipped or makes only a small investment ( {$300 for many readers). The larger number of potential users will make the system more cost effective and may partially offset the initially higher cost of a CD writer. A further consideration is that both Mac and IBM clone users need acess. The formatting for opticals is usually specific to one or the other of these standards (unless you plan to go with Apple PowerPCs) and will thus be more limited. Photo CDs can be read on drivers of either machine format with no conversions or translations required. We think that for these reasons, CDs are the way to go.
I have been requested to pass on the following message for discussion:
From: Bill Monroe Electron Microscope Center Mississippi State University
His Question: Occasionally our laboratory staff has experienced problems with thin sections coming off the grid during immuno-gold labeling steps. The tissue sections are from L.R. White embedded material and are mounted on 200 mesh formvar-coated nickel grids.
The Question: Are there any suggestions to remedy this problem of sections coming off the grid?
Richard Kuklinski, rfk2-at-ra.msstate.edu Electron Microscope Center, Mississippi State University
How do I persuade Image to measure the number of intercepts on a line or to measure the length of individual intercepts? Both of these measurements are useful in determining the grain size of metals, for example, see ASTM standard E112 and E1181.
We originally posted this to the NIH-Image list but received no responses, so if anyone can help, we'd appreciate it. Thanks.
You can reply to my e-mail address smiths-at-mlc.lib.mi.us OR directly to my patron at the following address:
Sam Purdy National Steel Corp. Technical Research Center 1745 Fritz Drive Trenton, MI 48183 313/676-2682 or FAX:313/676-2030
I am looking for the name and address of the manufacture of crystalbond. We had purchased in the past sticks about 3/4" in diameter at a very reasonable price (much below Gatan's price) but can't locate the source now.
Serial Sections? An easy way to keep serial sections attached to one another is to coat the block with a sticky substance. Previously, I used something called "Takki-wax". Does anyone know of a supplier for this wonderful stuff, we just ran out?
We are interested in collecting RHEED and LEED images with a video camera. Stuff like line profiles, spot intensity fluctuations. I checked into this several years ago and found a system by Staib Instruments It was a lot of money. I then thought of buying a system with a Sony XC-77 camera, Fuji CF50L lens, DT2851 Framegrabber, Halo 88, and Image Pro software. This sytem would have cost about $7K. Several years have past and now we have a Mac Quadra 840AV. Someone here thinks he can do the same thing with the Mac AV inputs that these other systems could do with dedicated boards. Any comments from people running such systems would be helpful.
} } Serial Sections? } An easy way to keep serial sections attached to one another is to coat the } block with a sticky substance. Previously, I used something called } "Takki-wax". Does anyone know of a supplier for this wonderful stuff, we just } ran out? } ================================================================
I have also heard of using diluted rubber cement for keeping serial sections together. ********************************************************** * Greg Erdos ** * * Director, ICBR EMCL ** Phone 904-392-1295 * * 218 Carr Hall ** FAX 904-392-8598 * * University of Florida ** gwerdos-at-gnv.ifas.ufl.edu * * Gainesville, FL 32611 ** * **********************************************************
We've been using the Becton-Dickinson mouse monoclonal antibody againsty bromodeoxyuridine (BrdU) for a few years for a variety of samples and fixatives. We'vew been using at 1/4000 successfully for ABC-peroxidase and fluorescently labelled secondary antibodies.
The BD is very pricey, $245 per bottle. Has anyone tried other brands of antibodies or fluorescently conjugated anti-BrdU antibodies? What dilutions did you use, how sensitive did you find them (especially the conjugated Ab)?
Glen MacDonald Hearing Development Laboratories RL-30 University of Washington Seattle, WA 98195 (206)543-8360 glenmac-at-u.washington.edu
Message-Id: {9403291926.AA22920-at-riker.ml.wpafb.af.mil} To: WALCKSD:ml:wpafb, SCHELTFJ:ml:wpafb Subj: Crystalbond Orig-Author: {"M. J. Kramer" {MJKRAMER-at-vaxld.ameslab.gov} }:ddn:wpafb ----------------------------------------------------------- I am looking for the name and address of the manufacture of crystalbond. We had purchased in the past sticks about 3/4" in diameter at a very reasonable price (much below Gatan's price) but can't locate the source now.
Re} I-G-labeled sections falling off Are the sections falling off or is it the formvar that is comming away from the grids? I've never had any problem with sections comming detached from formvar-carbon films.
} } I have been requested to pass on the following message for discussion: } } } From: Bill Monroe } Electron Microscope Center } Mississippi State University } } His Question: Occasionally our laboratory staff has experienced } problems with thin sections coming off the grid during immuno-gold } labeling steps. The tissue sections are from L.R. White embedded } material and are mounted on 200 mesh formvar-coated nickel grids. } } The Question: Are there any suggestions to remedy this problem of } sections coming off the grid? } } } Richard Kuklinski, rfk2-at-ra.msstate.edu } Electron Microscope Center, Mississippi State University ========================================================== REPLY
We have never experienced this problem. We do however pick up sections from above rather than from below thereby eliminating the possibility of trapping water between the section and the formvar film. I don't know if this would make any difference. ********************************************************** * Greg Erdos ** * * Director, ICBR EMCL ** Phone 904-392-1295 * * 218 Carr Hall ** FAX 904-392-8598 * * University of Florida ** gwerdos-at-gnv.ifas.ufl.edu * * Gainesville, FL 32611 ** * **********************************************************
Crystal bond is supplied and made by Universal Photonics 1-800-645-7173. Universal Photonics is a supplier of materials for lens grinding and polishing (used to be Universal Optics) and developed Crystal bond for holding prisms and crystals to the work tool as they are being lapped. Their catalog has a lot of interesting stuff of potential use to microscopists, but for years was out of print. I think you can get one now, however.
South Bay Technology produces 4 standard mounting waxes designed for mounting samples for precision lapping, polishing and cutting. SBT makes a wax which is essentially identical to Crystalbond 509. The only difference is that QuickStick 135 is very clear and does not have the amber color normally associated with Crystalbond.
QuickStick 135 is acetone soluble and has a flow point of 135 degrees C. QuickStick 135 is used extensively in TEM sample preparation operations and is supplied with all South Bay Technology TEM sample preparation equipment. Quick Stick 135 is packaged in a plastic tray consisting of 20 3" x .25" x .25" unwrapped sticks (Approx. 360 grams) for $40. It can also be supplied in the 7" long cardboard tubes as is Crystalbond or in a variety of other forms.
For information on our other mounting waxes, free wax samples or information on any of our sample preparation products, please contact me as follows:
David Henriks South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
TEL: 714-492-2600 Toll-free in USA: 800-728-2233 FAX: 714-492-1499
e-mail: via CompuServe at: David Henriks, 73531,1344
Bill Monroe asks about sections falling off of grids during immunostaining. I use uncoated gold grids (no grid induced astigmatism) with LR White or Spurr's resin. I section blocks, pick up sections and let dry at least one day before immunostaining. Once in a while a few sections might fall off but this is not a major problem. I also immerse the grids in all solutions on parafilm. Hope this helps.
Anybody out there have suggestions for measurement of particles from the TEM? The particles are thought to measure about 80 angstroms. We are assuming using 80-200,000x magnification and need very precise measurements. The commercial standards are only good up to 50,000x.
I appriciate any help!
Kathy Walters Central Electron Microscopy Researh Facility University of Iowa
Kathy, How about using carbon graphite at 3.4 Angstrom lattice spacing or there is a type of asbestos that gives 9 Angstrom lattice spacing. These could be used at 100KX or above and the mag accuracy of your TEM could then be calculated. Cal
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