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From: desclinj-at-ulb.ac.be (Desclin Jean)
Date: Wed, 1 Mar 1995 08:17:01 +0100 (MET)
Subject: to Barbara Hartman

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Hi Barbara!

I tried to no avail to send you the present message at your email
address: it stubbornly bounced back, so I eventually post it to the
microscopy list.
I apologize to the members of this list if they should feel this is
wasting bandwidth: just delete and go on to the next message ;-)

Barbara!
I am afraid I did not make my question clear enough ;-)
What I wanted to know was:
1) the testes you wish to fix are from what species ?

2) for what kind of microscopy are they to be processed ? I.e.
T.E.M., S.E.M., or light microscopy ?

3) What's the embedding medium?

4) in the case of light microscopy, do you contemplate using some special
technique (besides for recognizing the different stages of the
seminiferous epithelium) which would require that some specific
chemical would be either required or, on the contrary, avoided?

In my personal experience with rat testes and light microscopy (this
is rather very long ago: more than 30 years! :-( ), Bouin and other
picric acid containing variations thereof are not optimal fixatives
for preservation of the acrosomial apparatus of spermatids, which is
one of the features used by most classification methods (Clermont's
among others...).
The best fixatives for light microscopy of paraffin embedded tissue
are those containing potassium dichromate such as, for example,
Helly's fluid (Zenker + formaldehyde), but they also are followed, after
rinsing and dehydration, by shrinkage which is still more important than
that observed after Bouin's or Bouin-Hollande's (somewhat better) fluids.

BTW, what's wrong with picric acid? It is always shipped in 'moist
condition' in order to minimize hazards. I work in our lab since 1953,
with Bouin, Bouin-Allen, Bouin-Hollande, Dubosq-Brazil etc., every day,
and we never experienced any problem. We store the picric acid for our
needs in the form of a saturated aqueous solution which keeps for as long
as you will. This stock solution is then used for preparing all picric
acid containing fixatives.

There is no point in trying to eliminate the picric acid from the fixed
pieces of tissue before sectioning. If the presence of picric acid in the
slides should hamper subsequent staining (a rare occurrence, btw), then
the easiest and expeditious way to get rid of it is to pretend your tissue
was fixed with a sublimate containing fluid: at the rehydration step, your
slides should undergo treatment by alcoholic iodine (or lugol solution) and
sodium thiosulfate in the usual way and 'voila': no more picric acid on the
slides within 2 minutes!
Under no circumstances should the pieces of tissue fixed with a picric
acid containing fixative undergo rinsing in water! This is sheer heresy ;-)
because it induces a lot of swelling in the tissue before shrinking even
more during dehydration (I never found out which histologist ever advocated
such method, but I know quite a number of renowned ones who firmly condemned
it, and with good reason). If you insist on rinsing the pieces of testes after
fixation, then merely increasing the number of steps through 90x alcohol should
do the trick; you may even add a thin layer (1-2 mm thick) of lithium
carbonate on the bottom of the alcohol containing flasks: this helps
dissolving part of the picric acid away.

Possibly the method which should yield the best pictures has been documented
in a book entitled 'Histological and histopathological Evaluation of the
Testis', by Russell,Ettlin, Sinha Hikim and Clegg: Cache River Press 1990,
(15777 Bolesta, Box 129, Clearwater, Fl 34620): ISBN 09627422-0-1.

At the end of the book, there is an appendix about the materials and methods
which you might find useful (although perfusion fixation of rat testes is
among the most frustrating experiences I ever had, even with a lot of
heparin ). This would also require embedding in epoxy resin and making large
semi-thin sections, which I don't know whether you are prepared to do ;).
Glutaraldehyde certainly seems to me the best choice, but then paraffin
embedding would be ruled out (too hard and too brittle after paraffin
embedding).
HTH, and good luck.
Let me know about the outcome (good, I hope)
John
(correction for above glitch about the alcohol/ethanol: should read
alcohol 90 percent (in volume); I am still stuck with kermit and an
awfull editor :( ).



***********************************************************
* Jean C. Desclin (John), Associate Prof. of Histology *
* Laboratory of Histology - Faculty of Medicine *
* Brussels Free University (U.L.B.) *
* e-mail: desclinj-at-ulb.ac.be (internet) *
* snail mail: route de Lennik 808 *
* B - 1070 Brussels Belgium *
***********************************************************





From: desclinj-at-ulb.ac.be (Desclin Jean)
Date: Wed, 1 Mar 1995 08:46:40 +0100 (MET)
Subject: ignore, please

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this just is a test because it seems I am not able to reach the
list. Please ignore and delete. I apologize for the inconvenience.
desclinj-at-ulb.ac.be





From: jpawley-at-macc.wisc.edu (James Pawley)
Date: Wed, 1 Mar 1995 18:17:03 -0600
Subject: High resolution HVEM about to be trashed. Do you need it?

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The 1 MeV AEI EM-7 at the University of Wisconsin, Madison has been used
for the study of biological specimens for the past 23 years. In the early
1980's, almost every subsystem of this instrument (magnetic shielding and
ambient field, lens-current and HV stability, vibration isolation, LaB6
source, TV-interface, Axis-centered stereo-tilt stage, high-resolution
cold-stage etc.) was upgraded with a view to performing high-resolution
studies on frozen biological specimens. Images of oriented gold films at
that time showed 0.14 nm spots in bright field and using an 11 mm gap
(Pawley, J.B. (1984) Ultramicroscopy 13-4:387-406, describes most of these
improvements in detail.)

Because it seems probable that the present management of this instrument
will decide (has decided?) to decommission it in the very near future, (It
was almost scrapped over last Christmas), I am posting this notice in case
there are those that feel that the country has need of a second instrument
with the general specifications of the Berkeley ARM or even of the
very-stable Haefely 1-MEV supply that it contains.

The instrument is in operating condition, but, until recently, has received
less maintenance than it should have for the past five years or so.

The questions are:

1. Do you have important projects that require higher resolution (or a
large gap) than that available from 200-400kV instruments? (Keep in mind
that knock-on damage may be more severe at the higher voltage.)
2. Would you be willing to travel to Madison to obtain such facilities?
3. Would you be interested in relocating the instrument to a more
convenient location?

Bear in mind that, because the instrument requires a room 3 floors high and
a large (100 t) vibration-isolation block and also has substantial power
and cooling requirements, moving it would be a bit complex. However, it
could surely be done at less than 10% of $5M cost of the new Stuttgart 1.25
MeV instrument.

It would also need stage-rods more suited for material-science applications
but it is possible that the stage formerly fitted to the Cambridge HREM
could be fitted without great trouble as both instruments used the same
column.

The purpose of this communication is solely for the information of possible
users with the aim of avoiding anyone in the future saying "If only you had
told me?". I do not represent the Integrated Microscopy Resource where the
instrument is installed.

Please send any responses directly to me and do so ASAP:

jpawley-at-macc.wisc.edu (James Pawley)

***************NEW ADDRESS**************
Prof. James B Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr. Madison, Wisconsin, 53706.
JPAWLEY-at-MACC.WISC.EDU






From: Keith Moulding :      MCMOULDK-at-usthk.ust.hk
Date: 02 Mar 1995 15:38:58 +0800
Subject: Channel Software - EBSP

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Hi,

I have been trying to contact Niels-Henrik Schmidt (author of Channel). The
FAX number I have seems to be out of date.

Does anyone have Niels-Henrik Schmidt current FAX number and address?

Thanks in advance.


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Dr. Keith Moulding,
Materials Characterisation and Preparation Centre,
Hong Kong University of Science and Technology,
Clear Water Bay,
Kowloon,
Hong Kong.

Tel: (852) 2358 8724
Fax: (852) 2358 2451

E-mail: mcmouldk-at-usthk.ust.hk

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From: andreas.brech-at-bio.uio.no (Andreas Brech)
Date: Thu, 02 Mar 1995 09:14:14 +0100
Subject: subscribe

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Andreas Brech
Electron Microscopical Unit for Biological Sciences
Department of Biology, University of Oslo.
P.O.Box 1062 Blindern
N-0316 Oslo 3
Norway
Tel.: + 43-22 85 61 89 (work)
+ 43-22 43 83 23 (privat)
Fax.: + 43-22 85 47 26
e-mail.: abrech-at-bio.uio.no





From: andreas.brech-at-bio.uio.no (Andreas Brech)
Date: Thu, 02 Mar 1995 09:18:32 +0100
Subject: subscribe

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Please, I would like to join the list.
Andreas Brech
Electron Microscopical Unit for Biological Sciences
Department of Biology, University of Oslo.
P.O.Box 1062 Blindern
N-0316 Oslo 3
Norway
Tel.: + 43-22 85 61 89 (work)
+ 43-22 43 83 23 (privat)
Fax.: + 43-22 85 47 26
e-mail.: abrech-at-bio.uio.no





From: NICK SCHRYVERS :      nick_schryvers-at-ruca.ua.ac.be
Date: 2 Mar 1995 14:59:45 +0100
Subject: HREM versus high ground wat

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Message-Id: {n1417965773.25625-at-ematserv.ruca.ua.ac.be}

REGARDING HREM versus high ground water

Does anyone has experience with high ground water levels troubeling HREM
operation?
Nick Schryvers
EMAT, RUCA
Antwerp, Belgium





From: tivol-at-tethys.ph.albany.edu
Date: Thu, 02 Mar 1995 10:35:15 EST
Subject: Electron backscattering cross-sections

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Does anyone have a referrence for backscattering cross-sections of electrons
in various substances as a function of energy and/or of angle? I'd really
appreciate a reply either to the list, by email or if I could get a table by
fax at (518) 474-8590. TIA.
Yours,
Bill Tivol
tivol-at-tethys.ph.albany.edu




From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Thu, 2 Mar 1995 10:58:56 -500
Subject: Freeze-Fracture

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I'm looking for any recomendations for freeze-fracture units.
We're putting together a grant here to obtain one and the only souce
I've come up with is Bal-tec. I am looking for other possible
vendors. Apparently JEOL has stopped production of their freeze-
fracture unit. Specifically we're looking for units comparable to
the Bal-Tec BAF 060 (not that I as of yet have anything against the
BAF 060, but it is nice to look around first.)

Thank you.

Richard E. Edelmann
Electron Microscopy Facility Supervisor
Miami University, Oxford, Ohio




From: T. Page Owen Jr :      tpowe-at-conncoll.edu
Date: Thu, 2 Mar 1995 13:25:27 -0500 (EST)
Subject: Re: Freeze-Fracture

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Cressington makes a nice freeze-fracture machine. I don't have the
address at hand, but I believe they are based in England.

Page Owen
Dept. of Botany
Connecticut College
New London, CT 06320






From: Dennis Shubitowski :      dennis%odin-at-odin.morph.med.umich.edu
Date: Thu, 2 Mar 1995 15:43:04 -0500 (EST)
Subject:

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Errors-To: {dennis-at-odin.morph.med.umich.edu}






From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Thu, 2 Mar 1995 13:43:38 -0400 (EDT)
Subject: RE: Freeze-Fracture

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Message-Id: {9503022058.AA09986-at-julian.uwo.ca}


SUBJECT: Freeze-fracture
Riichard,

I don't think there is a unit out as yet that can match the specs
and design of the new BAL-TEC unit. Cressington is another company which
manufactures freeze-fracture unit. While it may not have all the features of
the new BAL-TEC one, it is reported to be good for routine freeze-fracture.

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Thu, 2 Mar 1995 11:58:20 -0700
Subject: Re: Freeze-Fracture

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Message-Id: {v01510100ab7bc4018e5c-at-[129.82.126.28]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} I'm looking for any recomendations for freeze-fracture units.
} We're putting together a grant here to obtain one and the only souce
} I've come up with is Bal-tec. I am looking for other possible
} vendors. Apparently JEOL has stopped production of their freeze-
} fracture unit. Specifically we're looking for units comparable to
} the Bal-Tec BAF 060 (not that I as of yet have anything against the
} BAF 060, but it is nice to look around first.)
}
} Thank you.
}
} Richard E. Edelmann
} Electron Microscopy Facility Supervisor
} Miami University, Oxford, Ohio

It looks as though you have some misinformation. JEOL is making the JFD
9000 series freeze-fracture instruments. I know that John Rash has
recently purchased and installed the 9010CR with the latest modifications,
including a specimen stage with rapid cooling.

Both Bal-Tec and JEOL make fine instruments and are worth looking at.


John chandler-at-lamar.ColoState.EDU Fort Collins CO






From: Jake Schaper :      Jake_Schaper-at-chdqm.sps.mot.com
Date: 2 Mar 1995 14:00:50 -0700
Subject: Subject-EM / AL/Si Stress

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Message-Id: {n1417969004.27274-at-chdqm.sps.mot.com}

Many published investigations on stress voids in aluminum/silicon alloys
report using CF4/O2 plasma as the deprocessing method to remove the final
PECVD oxynitride/ SiN. Does the use of this plasma chemistry generate its own
voids by removing silicon precipitates which in turn leave voids possibly
identified as stress voids? Precipitates that were in or near the correct
orientation give the appearance (typically wedge-shaped) of a stress void.
Optical inspection without deprocessing does not have adequate resolution.
I'd appreciate any comments on the CF4/O2 method and a possible alternative
method (other than argon backsputtering).

**********************************************************
Jake Schaper
Product Analysis Lab
Motorola
Chandler, Arizona
Phone 602-814-4756
**********************************************************






From: Dennis Shubitowski :      dennis%odin-at-odin.morph.med.umich.edu
Date: Thu, 2 Mar 1995 15:43:52 -0500 (EST)
Subject: Epoxy Resins: Accelerator?

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Errors-To: {dennis-at-odin.morph.med.umich.edu}

One of the divisions of our lab teases apart sural nerve tissue that have
been kept in a 1:1 mixture of epon:propylene oxide. The samples are very
easy to tease when the mixture is very fresh. The problem is that samples
became back-logged and were not able to be teased right away. A quick
decision had to be made, and the samples were frozen at -70C until they
could be teased.

The nerve fibers need to be infiltrated to be teased but not polymerized
or else they become too fragile. A problem is becoming evident that after a
long period of storage, the nerve samples are being fully infiltrated
and polymerized despite being frozen (which we thought would almost stop
the polymerization process because it is largely dependant on heat).
Realizing that hind-sight is 20/20, would it have been better to leave the
accelerator out of the epoxy mixture? What affect would this have on the
quality of the resin? I understand accelerator to act as a catalyst being
a part of the polymerization reaction but not really consumed. Any
opinions to offer?

Thanks,

Dennis








From: emlab-at-ucsco.ucsc.edu (Jon Krupp)
Date: Thu, 2 Mar 1995 11:12:14 -0800
Subject: Survey: Lab Changes

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Hi again:

I have been asked to make a brief (15 min) presentation at Scanning 95 in
Monterey, on March 30, 1995. The topic is 'Changing Role of the Microscopy
Lab in the University'. I know how my lab has changed, but would like to
get some idea from a broader sample of microscopists about changes in our
role etc.

My rough observations at this time include some of the following. More labs
seem to be consolidating, so we keep trying to catch up with too many
techniques, especially as staffs are reduced.

There are lots of other places for researchers to spend their funding than
before, we have lost lots of users in biology to molecular techniques. Some
of the new faculty see microscopy as 'too hard'.

There are fewer and fewer students who want to be 'microscopists'. Most
seem to want to come in on Monday, drop off something, and return on Friday
with a picture or two for their thesis.

The EM lab on our campus has evolved into an imaging and microscopy lab.
Because of our interest in images, we have always had the best darkrooms,
now we are trying to lead the way into digital imaging. This means more
computers and branching out from traditional 'microscopy'.

I could probably think of other changes, but that wouldn't leave anything
for you to add. Don't be shy, reply directly to me and I will try to
incorporate your comments in my presentation in a constructive way. Let me
know if you want specific credit for an idea, or if you prefer to have your
input blended for anonymity.

If you can't make it to Monterey, I'll send a summary if you ask.

Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95060
emlab-at-ucsco.ucsc.edu
(408) 459-2477






From: John F. Conroy :      John.Conroy-at-m.cc.utah.edu
Date: Fri, 24 Feb 1995 14:23:58 -0700 (MST)
Subject: Optical Microscopy of Tissues

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Reply to: RE} Re: Optical Microscopy of Tissues
Dear John,
Sorry about the delay in responding - I missed your message in the deluge I
received while visiting labs for a few days. Check out the following papers on
infrared DIC-videomicroscopy.

H.-U. Dodt, W. Zieglgansberger (1990) Brain Research 537:333-356; (1994) Trends
in Neurosciences 17 (11): 453-458 (and references therein).

They looked with transmitted light at depths of up to 100 um in 300 um thick
sections. Used a 63x 1.4 NA oil immersion objective and DIC optics to visualize
nerve terminals. Note that the Nikon 60x 1.2 NA WATER immersion objective is
now available and has advantages in deep sections (see for example Mel
Brenner's application note in the November 1994 Journal of NIH Research).

Infrared penetrates well because water and tissues doesn't absorb it as much as
visible. For video work a CCD (or cooled CCD) is used because they are
sensitive to IR (unlike most tube cameras and your eye). DIC is the method of
choice because of optical sectioning. A general review or IR cameras is:
Silverman et al (March 1992) Scientific American 78-83 and 112-113. Any high
quality video CCD camera will work (as long as it does not have an IR blocking
filter!).

Sincerely,

Dr. George McNamara
Universal Imaging Corporation
--------------------------------------

Hello,

I am currently writing a grant proposal, and I have been knocking my head
against the wall looking for some sort of data on the optical transmission
properties of nervous tissue (the brain). Does anyone have a good
reference, or, from a practical standpoint, how thick do brain tissue
slices have to be to obtain good optical transmission (esp. relative to
other fatty tissue slices like those from the earlobe, whose transmission
characteristics I can find since people have been doing pulse
oximetry.).

Thanks in advance,

John Conroy
University of Utah
Dept. Bioengineering

---------
Dear John,

It has been years since I worked in this area, but I suggest you might
look at the literature for the optical transmission properties of retina.
As a nervous tissue, its optical properties should be quite similar to the
CNS as long as the measurements are not made in the vicinity of the fovea.
And, of course, due the importance of retinal transparency for vision, its
optical properties must be well known. For a place to start I'd have a
look at the "Handbook of sensory physiology" Springer-Verlag.

Just my two cents worth, Steven






From: NICK SCHRYVERS :      nick_schryvers-at-ruca.ua.ac.be
Date: 3 Mar 1995 08:26:09 +0100
Subject: obj. lens 100C replacement

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REGARDING obj. lens 100C replacement

The objective lens and the upper and lower column parts of our 20 year old
side entry JEOL 100 C microscope need replacement. New parts, however, are
rather expensive in view of the age of the instrument so we're looking for
anyone who has some second hand spare parts for sale. Sincerely,
Nick Schryvers tel: 32-3-2180247 fax: 32-3-2180257
EMAT, University of Antwerp
Groenenborgerlaan 171
B-2020 ANTWERP
Belgium






From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Fri, 3 Mar 1995 17:37:52 +1100
Subject: Resins: BDMA vs DMP-30

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A message from Allan Mitchell:

The BDMA versus DMP-30 debate continues.

I first became aware of the recomendation to replace the epoxy resin=
acelerator DMP-30 with the acelerator BDMA when Audrey Glauert published=
her article "Accelerators for epoxy resins" in the RMS Proceedings (Sept, 1=
987).

The reason offered to encourage the switch from DMP-30 to BDMA was the fact=
that BDMA is less viscous, therefore diffuses into the tissue sample=
better. This gives a more even polymerisation and better sectioning. BDMA=
also has a longer shelf life.

I followed Glauert's recomendation, replacing DMP-30 with BDMA in our resin=
formula's. However, Audrey Glauert did not mention in the article that=
we should double the quantity of BDMA in the formulation when switching=
from DMP-30 to BDMA.

I replaced the DMP-30 with BDMA, using the BDMA at the same concentration as=
I had used the DMP-30.

Generally, it worked fine. Occasionally I had 'strange embedding' problems,=
ie samples not properly polymerised.

It then came to my attention that I should be using BDMA at twice the DMP-30=
concentration. I did not pay much attention to this at the time as=
generally everything had been working fine and I was to busy to experiment=
with the change.

After recent 'flow' on the List server the topic again surfaced i.e. I=
should be using BDMA at twice the DMP-30 concentration. This I have now tr=
ied.

My problem is that if I do an overnight infiltration in resin with the=
increased concentration of BDMA the resin is so viscous next morning it=
won't even come out of the vial when tipped upside down. To change to=
fresh resin is almost impossible, in fact it is easier to change the=
samples to fresh resin in a new processing tube. Unfortunately even then=
it is not totally successful as a large 'blob' of tacky resin stays with=
the sample.

I did a little experiment as outlined below;

1. Made up resin with normal DMP-30 concentration.
2. Made up resin with BDMA at normal DMP-30 concentration.
3. Made up resin with BDMA at twice DMP-30 concentration.

Results

Viscosity (by eye) Colour
To start with
DMP-30 most viscous slightly orange
BDMA (-at- DMP-30 conc) least viscous straw coloured
BDMA (-at- 2 x DMP-30 conc)similar to BDMA -at- DMP 30 conc straw coloured

After 5 hours
DMP-30 least viscous darker straw coloured
BDMA (-at- DMP-30 conc) similar to DMP-30 straw coloured
BDMA (-at- 2 x DMP-30 conc)quite viscous straw coloured

Overnight
DMP-30 least viscous straw coloured
BSMA (-at- DMP-30 conc) slightly more viscous than DMP-30 straw coloured
BDMA (-at- 2 x DMP-30 conc)very very viscous straw coloured

After the overnight step both the DMP-30 mix and the BDMA mix at the DMP-30=
concentration were quite acceptable, ie no difficulty replacing the old=
resin with fresh resin.

The BDMA mix at the twice DMP-30 concentration ws very very tacky.

After polymerisation both the DMP-30 and the BDMA -at- the DMP-30=
concentration are a light straw colour (normal expectation). The BDMA -at-=
twice the DMP-30 concentration was slightly darker, although still an=
acceptable straw colour.

By modifying my processing schedule such that the samples stay in 3 parts=
plastic and 1 part propylene oxide overnight I can successfully process a=
sample however, I am a little unhappy with the short time the sample is in=
'resin only' the following day, ie out of 3;1 and into fresh plastic first=
thing in the morning, embedded and in the oven before I go home. With a=
lot of samples to embed at times trying to give the samples as long as=
possible in the 'resin only' makes the end of the day a bit tight for time=
.

The problem becoming particularly accute when using our Lynx Automatic=
Tissue processor. If using BDMA -at- twice the DMP-30 concentration I cannot=
leave the resin in the processer overnight as it is to tacky the next day.

My Question ??? (at last some may say)

1. How are others out there, who are using BDMA at twice the DMP-30=
concentration getting around the problem of the resin going very tacky=
overnight, during infiltration?

What sort of infiltration times do you use?


2. Are any of you using BDMA at twice the DMP-30 concentration in an=
automatic tissue processor?

3. Is any one else having problems with the resin if made up with BDMA at=
twice the DMP-30 concentration?



Thank you in anticipation.


Allan Mitchell
South Campus Electron Microscope Unit
Department of Anatomy and Structural Biology
Otago Medical School










From: A. Kent Christensen :      akc-at-umich.edu
Date: Fri, 3 Mar 1995 08:21:39 -0500 (EST)
Subject: Re: Epoxy Resins: Accelerator?

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Dennis Shubitowski {dennis-at-odin.morph.med.umich.edu}

Dennis,

If you omit the accelerator, it would probably work well. We use
Epon-Araldite (Polyscience kit), and we routinely store the mixed resin
(Epon + Araldite + DDSA), without catalyst (DMP-30), in a 4 degree C
refrigerator. It will remain good for at least 6 months. When we are
ready to embed, we measure out whatever volume we want, add DMP-30 to
make 2%, mix, and it is fine. If you stored your nerve samples in 1:1
Epon (no accelerator):PO, and later decided to embed them, you could then
embed them by standard procedures with catalyzed resin. If you have any
questions about this, give me a call (763-1287), or drop over (2 blocks
away).

Kent

A. Kent Christensen
Department of Anatomy and Cell Biology
University of Michigan Medical School
Ann Arbor, MI 48109-0616
{akc-at-umich.edu}

----------------------------------------

On Thu, 2 Mar 1995, Dennis Shubitowski wrote:

} One of the divisions of our lab teases apart sural nerve tissue that have
} been kept in a 1:1 mixture of epon:propylene oxide. The samples are very
} easy to tease when the mixture is very fresh. The problem is that samples
} became back-logged and were not able to be teased right away. A quick
} decision had to be made, and the samples were frozen at -70C until they
} could be teased.
}
} The nerve fibers need to be infiltrated to be teased but not polymerized
} or else they become too fragile. A problem is becoming evident that after a
} long period of storage, the nerve samples are being fully infiltrated
} and polymerized despite being frozen (which we thought would almost stop
} the polymerization process because it is largely dependant on heat).
} Realizing that hind-sight is 20/20, would it have been better to leave the
} accelerator out of the epoxy mixture? What affect would this have on the
} quality of the resin? I understand accelerator to act as a catalyst being
} a part of the polymerization reaction but not really consumed. Any
} opinions to offer?
}
} Thanks,
}
} Dennis
}
}
}
}
}




From: gwerdos-at-gnv.ifas.ufl.edu (Greg Erdos ICBR EM Core Lab)
Date: Fri, 03 Mar 1995 09:09:34 -0500 (EST)
Subject: Re: Epoxy Resins: Accelerator?

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MICROSCOPY-at-AAEM.AMC.ANL.GOV
Cc: Dennis Shubitowski {dennis-at-odin.morph.med.umich.edu}
Message-id: {01HNOZPUD02Q93M845-at-gnv.ifas.ufl.edu}
MIME-version: 1.0
X-Mailer: Windows Eudora Version 1.4.4
Content-type: text/plain; charset="us-ascii"
Content-transfer-encoding: 7BIT

In reference to Dennis Subitowksi's question about expoxt
accelerator, I can say that we routinely exclude the accelerator during
infiltration with Epon, Epon/Araldite mixture and Epon substitutes and add
it only to the second pure resin infiltration step. This allows long
infiltrations in earleir steps with difficult specimens. When we have
compared adding it to all steps, the results seem to be the same.
Hope that helps

*****ORIGNINAL MESSAGE BELOW********
} One of the divisions of our lab teases apart sural nerve tissue that have
} been kept in a 1:1 mixture of epon:propylene oxide. The samples are very
} easy to tease when the mixture is very fresh. The problem is that samples
} became back-logged and were not able to be teased right away. A quick
} decision had to be made, and the samples were frozen at -70C until they
} could be teased.
}
} The nerve fibers need to be infiltrated to be teased but not polymerized
} or else they become too fragile. A problem is becoming evident that after a
} long period of storage, the nerve samples are being fully infiltrated
} and polymerized despite being frozen (which we thought would almost stop
} the polymerization process because it is largely dependant on heat).
} Realizing that hind-sight is 20/20, would it have been better to leave the
} accelerator out of the epoxy mixture? What affect would this have on the
} quality of the resin? I understand accelerator to act as a catalyst being
} a part of the polymerization reaction but not really consumed. Any
} opinions to offer?
}
} Thanks,
}
} Dennis
}
}
}
}
}
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv,ifas.ufl.edu
Gainesville, FL 32611





From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Fri, 3 Mar 1995 12:28:33 -0500
Subject: Electron Microscopes for Sale

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Interested parties:

Due to renovations within the department, and the purchase of a confocal
microscope, we have 2 transmission EMs in excess of our needs that we
would like to move. Both microscopes are in good working condition, one
has been under a service contract until this past year (though lightly
used). The scopes:

1. Hitachi H-600 STEM (the one under service contract until recently)

2. Zeiss EM-9S TEM

We are not quite at a "No reasonable offer refused!" price level, but we
would like to see these scopes get some more use and we're eager to make
use of the space they are now occupying. So....if you are at all
interested, contact me and we can discuss the particulars and terms
of sale.

TIA,

Phil Rutledge
voice: (410) 455-3582
Email: prutle1-at-gl.umbc.edu
Fax: (410) 455-3875




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Fri, 3 Mar 1995 10:40:16 -0500 (EST)
Subject: Re: Epoxy Resins: Accelerator?

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Dennis Shubitowski {dennis-at-odin.morph.med.umich.edu}

We had a similar experience some years ago. Not with nerve, but with
other material. I can offer the following humble comments.

1. As you learned, resin does polymerize in even at freezing
temperatures. We have not tried -70 C and you did not specify how far
below freezing you stored your specimens. We did not test exactly why,
but reasoned that since the reaction is highly exothermic and since both
resin and tissue are good insulators, it is probable that local areas are
heated enough to allow polymerization. I look forward to any comments on
the errors in our reasoning, since this was only a guess to explain the
results.
2. Leaving out the accelerator does tremendously slow down the reaction,
but polymerization still occurs in absence of accelerator, and even in
the cold, so don't store for grossly extended times. To polymerize
infiltrate tissue with fresh resin containing accelerator (we use 2
changes, 4 hours each) after thawing tissue. This works well, although
some areas may have obvious interfaces between resins of different
hardness.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Thu, 2 Mar 1995, Dennis Shubitowski wrote:

} One of the divisions of our lab teases apart sural nerve tissue that have
} been kept in a 1:1 mixture of epon:propylene oxide. The samples are very
} easy to tease when the mixture is very fresh. The problem is that samples
} became back-logged and were not able to be teased right away. A quick
} decision had to be made, and the samples were frozen at -70C until they
} could be teased.
}
} The nerve fibers need to be infiltrated to be teased but not polymerized
} or else they become too fragile. A problem is becoming evident that after a
} long period of storage, the nerve samples are being fully infiltrated
} and polymerized despite being frozen (which we thought would almost stop
} the polymerization process because it is largely dependant on heat).
} Realizing that hind-sight is 20/20, would it have been better to leave the
} accelerator out of the epoxy mixture? What affect would this have on the
} quality of the resin? I understand accelerator to act as a catalyst being
} a part of the polymerization reaction but not really consumed. Any
} opinions to offer?
}
} Thanks,
}
} Dennis
}
}
}
}
}





From: EMLAB-at-opus.mco.edu
Date: Fri, 03 Mar 1995 16:09:13 -0400 (EDT)
Subject: Tissue processors

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From: Michael Rock :      merock-at-u.washington.edu
Date: Fri, 3 Mar 1995 14:23:47 -0800 (PST)
Subject: Re: Resins: BDMA vs DMP-30

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Richard-
Why not try infiltrating your samples overnight in 100% resin with the lower
concentration of BDMA, then swithching to a "short" infiltration with
fresh resin made with 2X concentration BDMA. then embedding in the higher
concentration of BDMA in fresh resin?
just a hunch.
-Mike




From: JOHNA-at-SCI.WFEB.EDU
Date: Fri, 03 Mar 1995 17:14:50 -0400 (EDT)
Subject: BDMA vs DMP-30

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I performed a similar set of experiments: BDMA vs DMP-30. Although it
infiltrated specimens adequately and although they sectioned fine;
dealing with changes of 100% plastic that were sooooo viscous was more than
I could tolerate. In spite of it's initial higher viscosity and shorter
shelf life, I switched back to using DMP-30 in our PolyBed 812 resin.
Doing changes of 100% plastic is no longer a headache and something to be
dreaded. If someone has a solution to the overnight BDMA-plastic blob
problem, I'd like to hear as well.

Cheers...........JohnA

___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Experimental Biology |
| 222 Maple Avenue |
| Shrewsbury, MA 01545-2737 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfeb.edu |
| |
|_________________________________________________|





From: Greg Erdos ICBR EM Core Lab Univers :      GWERDOS-at-gnv.ifas.ufl.edu
Date: Fri, 03 Mar 1995 22:48:06 -0500 (EST)
Subject: Fw: Electron Microscopes

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Dear EM Brothers & Sisters,

This message came to me and I have no good answers. Any comments will
be appreciated and I will forwrad to the questioners
=
=Greg,
=
=Tom Mareci suggested that you might be able to give us some technical
=advice on EM's to help us solve a temporary problem we're having.
=
=We're wanting to move our 4.7T magnet to a location in the ARB basement
=120' from Dr. Craig Tisher's EM facility, which has a Zeiss EM 10 SEM
=and a Topcon/International Scientific Instruments DS-130C TEM. The SEM
=specs {= 3 mG AC and {= 5 mG DC. The TEM specs {=6 mG AC. Dr. Tisher
=is reluctant to have us install our magnet, which with shileding is
=calculated to produce a DC magnetic field of about 17 mG in the EM room.
=Tom and I are having a hard time understanding the DC spec, in light of
=the earth's magnetic field being 100x greater than the 5 mG spec.
=We are also finding it difficult to get good scientifically sound
=answers from the EM manufacturers (at least that we NMR people can
=understand!). Could you enlighten us?
=
=Our main questions are: Will our DC field of 17 mG really pose a problem
=for the EM's? If so, how can it be remedied? How much will it cost?
=How are these systems currently shielded/compensated for the earth's
=magnetic field? (My guess is that at most, a quick realignment of a
=passive shield will be all that's required, if there's an observable
=effect.)
=
=Secondarily, since before and after image quality may be the best bottom-
=line test of the effect (if any) of our magnet, what standard samples can
=you recommend for these Q.C. checks?
=
=Thanks for your advice and comments.
=
=-Richard Briggs
=
=tel: 395-0680 ext 54279 (55-4279)
=FAX: 395-0279
=pager: Shands # 3497
=e-mail: rbriggs-at-ufnmr.health.ufl.edu
=
=P.S.: You may not remember, but I met you at Tom's Christmas party.
=
******************************************************
* Greg Erdos ** *
* Director, ICBR EMCL ** Phone 904-392-1295 *
* 218 Carr Hall ** FAX 904-846-0251 *
* University of Florida ** gwerdos-at-gnv.ifas.ufl.edu *
* Gainesville, FL 32611 ** *
******************************************************************




From: images1-at-biosci.mbp.missouri.edu (Michael Stanley)
Date: Sat, 4 Mar 1995 12:15:37 -0600
Subject: sheep ovary

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Hello All,
I have a user that is attempting double-label fluroescence for
protein localization in sheep ovaries. The ovaries seem to auto-fluoresce
very strongly in the FITC channel with some signal in the Rhodamine as
well. They have talked to other researchers who have not seen this
problem. The tissue is fixed in Carnoy's and paraffin embedded.
Has anyone seen or heard of this particular tissue lighting up like
a Christmas Tree?
TIA,

C. Michael Stanley, Ph.D.
Coordinator, Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2 Tucker Hall
University of Missouri-Columbia
Columbia, MO. 65211
(314) 882-4895
fax= 314-882-0123






From: xin yang li :      xl48-at-uow.edu.au
Date: Sun, 5 Mar 1995 22:14:58 +1000 (EST)
Subject: subscribe

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Hi, everyone there.

I have changed my e-mail address because the up date of the system here. I'd
like to keep subscribing information from you.

My old address:g9177248-at-wumpus.cc.uow.edu.au

My new address:xl48-at-wumpus.cc.uow.edu.au

Thanks

Xinyang Li




From: MatlsMicrs-at-aol.com
Date: Sun, 5 Mar 1995 14:39:46 -0500
Subject: Materials Microscopy

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In response to my invitation for a free subscription to Materials Microscopy
(posted on Feb. 24), we have been receiving a large number of requests and
inquiries on a daily basis via e-mail and FAX. We will try to process all
your subscription requests prior to the circulation of our next issue. Due
to the high volume of mail we have received, I cannot respond to your
inquiries individually. However, I have tried to summarize, itemize, and
respond to your most frequently asked questions as follows:
a. Materials Microscopy is published and circulated at no cost among the
materials microscopy community members by Promotech Associates, Inc.
b. Our publication is mainly concerned with providing articles and material
of interest to working materials microscopist. We would appreciate your
contribution which meets this requirement. Further information in this
regard can be obtained by contacting our technical editor, Dr. Patricia Labun
(a materials microscopist).
c. I will send a copy of our "rate sheet" to those of you who expressed
interest in placing ads or promotional material. Please feel free to contact
me if you require any additional info.
Thank you all for your interest in Materials Microscopy.

Rene E. Nicholas
Circulation/Sales Manager

Materials Microscopy
P.O. Box 2014
Scottsdale, AZ 85252

TEL (602) 947-7603
FAX (602) 947-7615
MatlsMicrs-at-aol.com




From: A. Kent Christensen :      akc-at-umich.edu
Date: Sun, 5 Mar 1995 15:52:57 -0500 (EST)
Subject: Re: Resins: BDMA vs DMP-30

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MICROSCOPY-at-AAEM.AMC.ANL.GOV

I'm wondering if the possible advantages of BDMA (lower viscosity,
longer shelf life) are worth all this. I'm still using a 1989 Polysciences
Epon-Araldite kit (with DMP-30) that still gives excellent results with
standard procedures.

A. Kent Christensen
Department of Anatomy and Cell Biology
University of Michigan Medical School
{akc-at-umich.edu}




From: desclinj-at-ulb.ac.be (Desclin Jean)
Date: Mon, 6 Mar 1995 08:25:18 +0100 (MET)
Subject: fixation of testes

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Hi Barbara!
I tried to no avail to send you the present message at your email
address: it stubbornly bounced back, so I eventually post it to the
microscopy list.
I apologize to the members of this list if they should feel this is
wasting bandwidth: just delete and go on to the next message ;-)

Barbara!
I am afraid I did not make my question clear enough ;-)
What I wanted to know was:
1) the testes you wish to fix are from what species ?

2) for what kind of microscopy are they to be processed ? I.e.
T.E.M., S.E.M., or light microscopy ?

3) What's the embedding medium?

4) in the case of light microscopy, do you contemplate using some special
technique (besides for recognizing the different stages of the
seminiferous epithelium) which would require that some specific
chemical would be either required or, on the contrary, avoided?

In my personal experience with rat testes and light microscopy (this
is rather very long ago: more than 30 years! :-( ), Bouin and other
picric acid containing variations thereof are not optimal fixatives
for preservation of the acrosomial apparatus of spermatids, which is
one of the features used by most classification methods (Clermont's
among others...).
The best fixatives for light microscopy of paraffin embedded tissue
are those containing potassium dichromate such as, for example,
Helly's fluid (Zenker + formaldehyde), but they also are followed, after
rinsing and dehydration, by shrinkage which is still more important than
that observed after Bouin's or Bouin-Hollande's (somewhat better) fluids.

BTW, what's wrong with picric acid? It is always shipped in 'moist
condition' in order to minimize hazards. I work in our lab since 1953,
with Bouin, Bouin-Allen, Bouin-Hollande, Dubosq-Brazil etc., every day,
and we never experienced any problem. We store the picric acid for our
needs in the form of a saturated aqueous solution which keeps for as long
as you will. This stock solution is then used for preparing all picric
acid containing fixatives.

There is no point in trying to eliminate the picric acid from the fixed
pieces of tissue before sectioning. If the presence of picric acid in the
slides should hamper subsequent staining (a rare occurrence, btw), then
the easiest and expeditious way to get rid of it is to pretend your tissue
was fixed with a sublimate containing fluid: at the rehydration step, your
slides should undergo treatment by alcoholic iodine (or lugol solution) and
sodium thiosulfate in the usual way and 'voila': no more picric acid on the
slides within 2 minutes!
Under no circumstances should the pieces of tissue fixed with a picric
acid containing fixative undergo rinsing in water! This is sheer heresy ;-)
because it induces a lot of swelling in the tissue before shrinking even
more during dehydration (I never found out which histologist ever advocated
such method, but I know quite a number of renowned ones who firmly condemned
it, and with good reason). If you insist on rinsing the pieces of testes after
fixation, then merely increasing the number of steps through 90pc x alcohol should
do the trick; you may even add a thin layer (1-2 mm thick) of lithium
carbonate on the bottom of the alcohol containing flasks: this helps
dissolving part of the picric acid away.

Possibly the method which should yield the best pictures has been documented
in a book entitled 'Histological and histopathological Evaluation of the
Testis', by Russell,Ettlin, Sinha Hikim and Clegg: Cache River Press 1990,
(15777 Bolesta, Box 129, Clearwater, Fl 34620): ISBN 09627422-0-1.

At the end of the book, there is an appendix about the materials and methods
which you might find useful (although perfusion fixation of rat testes is
among the most frustrating experiences I ever had, even with a lot of
heparin ). This would also require embedding in epoxy resin and making large
semi-thin sections, which I don't know whether you are prepared to do ;).
Glutaraldehyde certainly seems to me the best choice, but then paraffin
embedding would be ruled out (too hard and too brittle after paraffin
embedding).
HTH, and good luck.
Let me know about the outcome (good, I hope)
John



***********************************************************
* Jean C. Desclin (John), Associate Prof. of Histology *
* Laboratory of Histology - Faculty of Medicine *
* Brussels Free University (U.L.B.) *
* e-mail: desclinj-at-ulb.ac.be (internet) *
* snail mail: route de Lennik 808 *
* B - 1070 Brussels Belgium *
***********************************************************





From: Marcel.Paques-at-2488PAS.urlnl.sprint.com
Date: Mon, 6 Mar 1995 04:42:00 -0500
Subject: freeze fracture

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X400-Received: by mta merit in /PRMD=internet/ADMD=telemail/C=us/; Relayed; Mon, 6 Mar 1995 04:53:08 -0500
X400-Received: by /ADMD=TELEMAIL/C=US/; Relayed; Mon, 6 Mar 1995 04:38:08 -0500
X400-Received: by /PRMD=SMXFL2/ADMD=TELEMAIL/C=US/; Relayed; Mon, 6 Mar 1995 04:42:47 -0500
X400-Received: by /PRMD=LANGATE/ADMD=TELEMAIL/C=GB/; Relayed; Mon, 6 Mar 1995 04:42:00 -0500

Cressington makes an excellent freeze fracture machine. Suitable for
routine work and high resolution shadowing at very low temperatures.
In particular the reproducibility, even of W/Ta, is very good, and
attainable for everyone (user friendly).

The address is:
Dr Peter A Walley
CRESSINGTON Scientific Instruments Ltd
24 Chalk Hill Watford Herts WD1 4BX UK
Tel 0923 220499
Fax 0923 816646

Success

Marcel Paques
Unilever Research Laboratory Vlaardingen
The Netherlands
E-mail: Marcel.Paques-at-2488PAS.URLNL.Sprint.Com





From: Kevin H Jennings :      Kevin_H_Jennings%notes-at-sb.com
Date: 6 Mar 95 13:28:00 ES
Subject: freeze-fracture

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Message-Id: {9503061636.AA1118-at-pho018.sb.com}
To: Microscopy {Microscopy-at-aaem.amc.anl.gov}

Further to the comments made concerning Cressington - they also have a US
distributor:

Alan Berginc
Cressington Scientific Instruments
508 Thomson Drive
Cranberry TWP
Pittsburgh
PA16066-6425

Tel: 412-772-0220
Fax: 412-772-0219

We use a Cressington CFE- 50B which has proved excellent for reproducible
rotary low-angle shadowing of peptides. The system is also very flexible for
both W/Ta and Pt/C operation and is easily re-configured for a variety of
research applications.


Kevin Jennings
SmithKline Beecham Pharmaceuticals UK
Microscopy & Flow Cytometry Section
The Frythe
Welwyn
Hertfordshire
AL6 9AR
United kingdom

e-mail Kevin_H_Jennings%Notes-at-SB.com





From: EMLAB-at-opus.mco.edu
Date: Mon, 06 Mar 1995 09:22:27 -0400 (EDT)
Subject: TISSUE PROCESSORS

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Dear biological TEM'ers,

I am in the info gathering stage of purschasing a tissue processor for
EM samples. I have info on the Reichert-Lynx unit and the one made by RMC.
How do you like these units? Comments on advantages and disadvantages are
welcome.
I also have brouchers on a unit made by Sakura Finetek USA, Inc..
The phone number I called is no longer in working order.(213-539-5441)
Is this company still around? These brouchers are probably from the early 80's.
Are the any other companys that made tissue processors?

Thanks in advance

Ed Calomeni
Medical College of Ohio
Toledo, OH

emlab-at-opus.mco.edu

PS Sorry about the post on last friday with no message in the body. Computers
can be such good friends.




From: Christine Powers :      cp-at-insitu.ummed.edu
Date: Mon, 6 Mar 1995 10:08:00 -0500 (EST)
Subject: TEM-Cell culture monolayers

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I recently read W. L. Steffens comments regarding the use of Leighton
tubes for embedding cell cultures. Has anyone embedded the coverslips from
these tubes in LRWhite resin? I've been having trouble getting consistent
polymerization of monolayers on other coverslips (eg Thermanox) and am in
search of a new method for obtaining "en face" sections of monolayers for
immunocytochemistry. TIA for any hints on the topic.

Christine Powers
Dept. of Cell Biology
University of Massachusetts Medical Center
Worcester, MA 01655




From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Mon, 6 Mar 1995 10:06:02 EST
Subject: Tissue Processors

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Ed,
I can comment on the Lynx tissue processor which we have been using
since it first came out, about 8 years ago. The Lynx is made in
Australia, and was originally distributed in this country by Fairleigh-
Dickinson Laboratories until the distributorship was snatched up by
Reichert (now Leica). We paid about 5.5K for the unit and have had little
trouble with it aside from a blown power supply (they have upgraded this)
and a defective exhaust fan (we fixed this). We use it routinely for
processing TEM (epon and L.R. White) and SEM (through dehydration). It
works very well is quite consistent.
My only complaint involves the expendables for it...vials, caps,
baskets, etc. Originally, it was economical to use them once and dispose
of them as is intended. Once Leica became the distributor, the price of
the expendable essentially tripled...some things quadrupled. As a result,
we clean and recycle these components for as long as we can.
Additionally, the quality control of these items has suffered immensely.
There are now occasional vials that are distorted and won't fit the
turntable, and lids and baskets with burrs that must be filed.
If you can accept this, I still think that for the money (nearly 9K by
now?) its the best one out there. Good Luck.

-=W.L. Steffens=-
College of Veterinary Medicine
University of Georgia




From: Beth Trend :      trend-at-cems.umn.edu
Date: Mon, 6 Mar 1995 11:07:06 -0600
Subject: TEM: JEOL100CX for sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a JEOL 100CX scanning transmission electron microscope that we no=
longer
need because of more recent acquisitions. It was maintained continuously=
during
operation here and was in perfect working condition before we turned it o=
ff in =

July, 1994. =


This is a conventional scanning transmission electron microscope with =

resolutions of 3.4 =81 TEM.

It has specimen holders for heating-cooling, double tilt (tilt range is =B1=
45=A1), =

or rotation. =

We would be deligted to entertain offers for it.

Please contact Beth Trend if you are interested.




______________________________________________***************************=
*******
Beth Trend trend-at-cems.umn.edu or btrend-at-maroon.tc.=
umn.edu
Coordinator, Characterization Facility University of Minnesota
Center for Interfacial Engineering =

100 Union St SE Room 187 phone 612-624-1365 fax 612-626-7530=

Minneapolis, MN 55455 =






From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Mon, 6 Mar 1995 12:13:29 -0600
Subject: BDMA vs DMP-30 summary

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In July and September of 1994, there was some disciussion of BDMA vs. DMP-30. I
saved and consolidated 13 of those messages into a single document. If anyone is
interested in getting a copy, send me your e-mail address and I will zip one out
to you within a day or two.

--

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: Alec Madsen :      alecm-at-u.washington.edu
Date: Mon, 6 Mar 1995 10:48:35 -0800 (PST)
Subject: PC diffraction programs

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X-Sender: alecm-at-homer11.u.washington.edu

Folks,
I'm aware of several programs for the Mac that simulate
diffraction patterns and stereographic projections, etc. How about
similar programs for the PC? Thanks in advance.

Alec Madsen
alecm-at-u.washington.edu
Seattle, Wa




From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Mon, 06 Mar 1995 15:21:18 -0500
Subject: Wall Street Journal: Thermo buys Fisons

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Message-Id: {199503062018.PAA14966-at-totalrecall.rs.itd.umich.edu}

Well it may not sopund exciting to microscopists until you note that it
tranlates into
Noran's parent company buys Kevex's parent company!
March 3rd issue of Wall Street Journal. Thermo Instrument Systems to
acquire Fisons PLC's scientific instrument division. Cost $320million!
Anyone know whether this covers VG too?

Just thought you guys might like to know.

--
John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 6 Mar 1995 14:38:46 -0600 (CST)
Subject: PC Programs for Diffraction

Contents Retrieved from Microscopy Listserver Archives
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There are at least 1 or 2 old diffraction programs
for the PC in the EMMPDL library. You may access
it by Anonymous FTP at:

WWW.AMC.ANL.GOV

Username=Anonymous
Password=Your Email Address..


Nestor
Your Friendly Neighborhood SysOp.............




From: John Phelps :      phelps-at-ENH.NIST.GOV
Date: Mon, 06 Mar 1995 14:37:36 -0700 (MST)
Subject: terminal emulators

Contents Retrieved from Microscopy Listserver Archives
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Hi,
I know this is a little of the subject, but can anyone suggest a ftp site
that has terminal emulators. We need to emulate a tektronics 4205 terminal
to use our new xrd software. Any suggestions would be appreciated.

thanks,
John

John Phelps
NIST - Boulder, CO
303-497-7570




From: Fred Hayes :      fahayes-at-ucdavis.edu
Date: Mon, 6 Mar 1995 13:34:13 -0800 (PST)
Subject: used equipment

Contents Retrieved from Microscopy Listserver Archives
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I am looking for the following pieces of used equipment either in good
working order, fair condition and/or in need of minor repair:

1) glass knife breaker for LM microtomy
2) embedding oven (35-80C)
3) balance (top loading or mechanical)

Contact:

Fred A. Hayes 916-752-7712 work
University of California,Davis 916-752-4701 work
School of Medicine
Department ofMedical Pathology; EM Lab
MSIA E-mail:
Davis, CA 95616 fahayes-at-ucdavis.edu

1320 Dogwood Court 916-678-6280 home
Dixon, CA 95620-3227







From: John Phelps :      phelps-at-ENH.NIST.GOV
Date: Mon, 06 Mar 1995 15:44:30 -0700 (MST)
Subject: terminal emulators

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I know this is a little off the subject, but can anyone suggest a ftp site
that has terminal emulators. We need to emulate a tektronics 4205 terminal
to use our new xrd software. Any suggestions would be appreciated.

thanks,
John

John Phelps
NIST - Boulder, CO
303-497-7570




From: gwerdos-at-gnv.ifas.ufl.edu (Greg Erdos ICBR EM Core Lab)
Date: Mon, 06 Mar 1995 10:30:32 -0500 (EST)
Subject: Re: obj. lens 100C replacement

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Microscopy Mail {Microscopy-at-aaem.amc.anl.gov}
Message-id: {01HNT9F975CY93NYWI-at-gnv.ifas.ufl.edu}
MIME-version: 1.0
X-Mailer: Windows Eudora Version 1.4.4
Content-type: text/plain; charset="us-ascii"
Content-transfer-encoding: 7BIT

At 08:26 AM 3/3/95 +0100, NICK SCHRYVERS wrote:
} REGARDING obj. lens 100C replacement
}
} The objective lens and the upper and lower column parts of our 20 year old
} side entry JEOL 100 C microscope need replacement. New parts, however, are
} rather expensive in view of the age of the instrument so we're looking for
} anyone who has some second hand spare parts for sale. Sincerely,
} Nick Schryvers tel: 32-3-2180247 fax: 32-3-2180257
} EMAT, University of Antwerp
} Groenenborgerlaan 171
} B-2020 ANTWERP
} Belgium
**********************************************************
There is a JEOL 100 C that may be headed for the scrap heap. They
might be willing to give you parts. Contact Dr. Henry Aldrich, P.O. Box
110700, University of Florida, Gainesville, FL 32611 USA FAX 904-392-5922
(no e-mail) or his department chairman, Edward Hoffmann, at the same
address and FAX number who gets E-mail at EMH-at-GNV.IFAS.UFL.EDU


Good Luck
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv,ifas.ufl.edu
Gainesville, FL 32611





From: HUANGY-at-PHYAST.LA.ASU.EDU
Date: Mon, 6 Mar 1995 17:15:27 -0700 (MST)
Subject: change address

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


please change my address to yi_huang-at-qmgate.anl.gov. I'd like to continue the
subscription at the new address. Thank you.
Yi Huang
Argonne National Lab
building 212/c221
building 212/c221




From: mrdunlap-at-ucdavis.edu (Michael Dunlap)
Date: Mon, 6 Mar 1995 16:57:54 -0800
Subject: RE:Thermo buys Fisons

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X-Sender: szmdunla-at-bullwinkle.ucdavis.edu
Message-Id: {ab815e0000021004c2be-at-[128.120.187.4]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

John-

All instruments divisions of fisons are going to Thermo Instrument Systems
including VG.

Just what we all needed. I sure service and parts are going to be even
easier to get.

Mike

---------------------------------------------------------------------------
| Michael Dunlap | lab (916) 752-0284 |
| Facility For Advanced Instrumentation | fax (510) 422-2282 |
| University of California | mrdunlap-at-ucdavis.edu |
| Davis CA, 95616 | |
===========================================================================






From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Mon, 6 Mar 1995 10:46:32 EST
Subject: TEM of Cell Culture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To Christine Powers,

We have successfully used LR White resin for embedment of monolayers
grown in Leighton tubes. The problems encountered were the same ones you
encounter when using LR White in open molds or silicone rubber molds, or
just about anything other than gelatin capsules. It's most convenient to
embed coverslips in flat, open molds such as the silicone rubber type. We
had such problems with this (ie excluding oxygen, penetration of the molds
by the resin, etc) that we began embedding the coverslips standing upright
in gelatin capsules. The long narrow coverslips of the Leighton tubes are
just accommodated by a 00 gelatin capsule. This neccessitates
considerably more hand trimming to get to the monolayer profile, but
embedding problems are eliminated.
I might also add, that once the block face is prepared for cutting,
the exposed coverslip may be peeled away from the embedded cells, greatly
facilitating cutting. If you do this, I would also recommend mounting the
sections on a support filmed grid, as the cells will be on the edge of the
section.
Good Luck!

-=W.L. Steffens=-
College of Veterinary Medicine
University of Georgia




From: Software department :      software-at-oimag.win-uk.net
Date: Mon, 06 Mar 1995 17:46:23
Subject: Job Opportunities/Microanalysis Software

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X-Mailer: WinNET Mail, v2.30
Message-ID: {463-at-oimag.win-uk.net}
Reply-To: Software department {software-at-oimag.win-uk.net}
To: microscopy-at-aaem.amc.anl.gov

The following advertisement has recently been placed in the press
and may be of interest to those who read this Newsgroup who would
like to work in the UK:

SOFTWARE DEVELOPMENT FOR MICROANALYSIS
The Software team at Oxford Instruments Microanalysis Group develop
high quality instrumentation products to satisfy customers in an
international marketplace. Currently we have a number of vacancies
for individuals who would enjoy the challenge of solving complex
problems to generate leading edge products. If you are competent in
Visual Basic, C++ or C, have a basic understanding of electronics and
computer interfacing and feel you could make a strong contribution in
any or all of the following areas please contact us:

Practical applications experience using an SEM or microprobe.
Knowledge of x-ray microanalysis and familiarity with operation of WD spectrometers.
Experience in project management and identifying the "voice of the customer".
Practical use of applied mathematics, numerical methods, chemometrics.
Experience with real time control and hardware automation.

Successful applicants will receive an attractive benefits package
and salary commensurate with capability.

Please send a copy of your CV, and a telephone contact number to

Peter Statham ,
Oxford Instruments Microanalysis Group
Halifax Road, High Wycombe, Bucks HP12 3SE England
Telephone (01494) 442255 Fax (01494) 461033
email: software-at-oimag.win-uk.net


-----------------------------------------------------------------
Please reply to this e-mail with the name of the person you
wish to recieve it in the subject (i.e. FAO John Smith), as this is
a shared e-mail address.





From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Mon, 6 Mar 1995 14:41:40 -0500
Subject: Job Posting

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X-Sender: jfmjfm-at-srvr5.engin.umich.edu
Message-Id: {ab811569160210033ef2-at-[141.212.196.13]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

The following is a copy of the job posting that recently appeared on the
University of Michigan Gopher Server UM GopherBlue.

The full URL is:
gopher://gopher.itd.umich.edu:8888/00/acadaff/hris/gopher/Job%20Postings/Pro
fessional%5CAdministrative/-at-39-at-RES%20ASSOC%20II%20ENGR%20%28Materials%20Scie
nce%20%26%20Engineering%29


Job Title: RES ASSOC II ENGR Grade: 09 Min/Max $ 25,500/ 64,500
Posting Number: T-95-0826-LM Materials Science & Engineering
Open Date: 03/06/1995 Close Date: 03/10/1995
Jobclass: 12871 Hours: 40.00

DUTIES:
Operate and train users in the use of the lab instruments: a JEOL
200FX Analytical Electron Microscope, a JOEL 4000EX High Resolution
electron Microscope, a Philips EM420 Transmission Electron Microscope,
an ElectroScan E3 Environmental Scanning Electron Microscope, a
Digital Instruments Scanning Force Microscope, a PHI 5400 X-ray
Photoelectron Spectrometer and a PHI 600 Scanning Auger Microprobe;
the successful candidate will not necessarily need to be proficient in
the use of all instruments specified, but familiarity with at least
four is essential; other duties include: aiding users with reduction
and analysis of data recorded on lab instruments; aiding users with
sample preparation equipment and preparing samples; help other lab
staff maintain lab equipment; maintain lab darkroom and supplies; take
part in collaborative projects with other members of the University;
develop personal research projects as time permits.

DESIRED QUALIFICATIONS:
Knowledge of Analytical Electron Microscopy, Scanning Transmission
Electron Microscopy and High Resolution Electron Microscopy; TEM
expertise should include detailed understanding of diffraction
contract, defect imaging, selected area diffraction, high resolution
imaging, high resolution image simulation and analysis; experience
with computer programming in FORTRAN, C or Pascal.

MINIMUM QUALIFICATIONS:
Master's degree or equivalent in a scientific related field;
demonstrated prior related work experience; familiarity with computer
systems such as Apple Macintoshes, PCs and/or UNIX workstations;
working knowledge of general transmission electron microscopy and
scanning electron microscopy; experience with standard data analysis
for analytical techniques such as X-ray energy dispersive spectroscopy
(XEDS), electron energy loss spectroscopy (EELS) and micro
diffraction; good interpersonal skills.


---------------------------------------------------------------------------

Present Applications For This Position To The Following Office:

Ann Arbor Campus Employment Services Office
Room G250 Wolverine Tower
3003 South State Street
Ann Arbor, Michigan 48l09

(313) 764-6580

8 AM to 5 PM, Monday - Friday



John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html






From: desclinj-at-ulb.ac.be (Desclin Jean)
Date: Tue, 7 Mar 1995 11:00:54 +0100 (MET)
Subject: testis fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hello Barbara!
I am afraid that, under the conditions you describe for fixation,
significant shrinkage should be unavoidable whatever fixative
would be used.
I already mentioned that picric acid 'per se' in the fixing fluid
does not ensure adequate preservation of acrosomial structure which
is required for easy classification of spermatids (and thus of
stages of the seminiferous epithelium.)
What should be avoided in any case is the presence of acids usually
part of most recipes for fixatives, such as acetic acid and the like.
If you use '10 percent formalin', you may experience some problems
because you in fact never know the actual composition of such
solution: it polymerizes on the shelf and becomes also quite acidic!
You should prepare a solution of phosphate buffered (pH 7.2) 4 percent
formaldehyde from paraformaldehyde powder on the same day (or the
previous one) that you are sacrificing the animals (you should find
recipes for making such solution in any practical handbook for
microscopical technique ;-)).

Fixation through immersion of the testes in the fixative may be
almost acceptable for mice testes because they are small; this won't
be the case for other species, even for the rat. You may try to make
small (with caution!) incisions in the albuginea with a very sharp
razor blade when the testes have stayed for at least an hour in the
fixative, in order to improve penetration of the formaldehyde.
Another trick to improve diffusion and fixation is to add from 0.5 to
1.0 percent (in volume) of DMSO (dimethylsulfoxide) to the fixing
fluid (best is to combine both...)

BUT,LAST and PROBABLY _MOST_ IMPORTANT: you should realize that
formaldehyde fixation, i.e. crosslinking, proceeds exceedingly
slowly! Therefore, tissues should stay in the fixative at least
two weeks (one year or more won't harm!) before being further
processed (two weeks probably is a conservative estimate). Assessment
of pathological tissues fixed for only a few hours in NBF may
frequently be required for practical reasons, but don't expect such
methods to result in nice preservation of testis tissue, especially
if you need estimating quality of spermatogenesis!

You also may try to enhance fixation - and shorten the duration
required for fixation - by performing it in the microwave oven: several
cycles of no more than one minute at a time (perhaps 10 times, tissue
temperatures exceeding 50 degrees celsius should be avoided), if
you are indeed in a hurry and can't wait several weeks for
fixation to proceed at its usual pace. If you try this, don't forget
to place a water load (a becher container with about 750-1000 ml
of water) besides your fixative containing flask in the oven.

That's all what I can think of. HTH
Good luck
John


***********************************************************
* Jean C. Desclin (John), Associate Prof. of Histology *
* Laboratory of Histology - Faculty of Medicine *
* Brussels Free University (U.L.B.) *
* e-mail: desclinj-at-ulb.ac.be (internet) *
* snail mail: route de Lennik 808 *
* B - 1070 Brussels Belgium *
***********************************************************




From: gwerdos-at-gnv.ifas.ufl.edu (Greg Erdos ICBR EM Core Lab)
Date: Tue, 07 Mar 1995 09:38:54 -0500 (EST)
Subject: JEOL 100 C parts

Contents Retrieved from Microscopy Listserver Archives
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Regarding the following message:

I was unable to reach Nick Schryvers at his E-mail address therefore
my reply is below. Perhaps others will be interested:

Nick writes:
} } } The objective lens and the upper and lower column parts of our 20 year old
} } } side entry JEOL 100 C microscope need replacement. New parts, however, are
} } } rather expensive in view of the age of the instrument so we're looking for
} } } anyone who has some second hand spare parts for sale. Sincerely,
} } } Nick Schryvers tel: 32-3-2180247 fax: 32-3-2180257
} } } EMAT, University of Antwerp
} } } Groenenborgerlaan 171
} } } B-2020 ANTWERP
} } } Belgium
} } **********************************************************

} } My reply:

There is a JEOL 100 C that may be headed for the scrap heap. They
} } might be willing to give you parts. Contact Dr. Henry Aldrich, P.O. Box
} } 110700, University of Florida, Gainesville, FL 32611 USA FAX 904-392-5922
} } (no e-mail) or his department chairman, Edward Hoffmann, at the same
} } address and FAX number who gets E-mail at EMH-at-GNV.IFAS.UFL.EDU
} }
} }
} } Good Luck
} } -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
} } Greg Erdos Phone: 904-392-1295
} } Scientific Director, ICBR EMCL
} } 218 Carr Hall Fax 904-846-0251
} } University of Florida E-mail: gwerdos-at-gnv,ifas.ufl.edu
} } Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv,ifas.ufl.edu
Gainesville, FL 32611





From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Tue, 7 Mar 1995 10:38:23 -0500 (EST)
Subject: Re: testis fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I concur with Dr. Desclin's suggestions- however, I would add that pH and
osmolarity of your solutions is easy to do, and can be helpful.
Perfusion fixation of the testis is also a possibility. Those are my
humble additions-


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Tue, 7 Mar 1995, Desclin Jean wrote:

}
}
} Hello Barbara!
} I am afraid that, under the conditions you describe for fixation,
} significant shrinkage should be unavoidable whatever fixative
} would be used.
} I already mentioned that picric acid 'per se' in the fixing fluid
} does not ensure adequate preservation of acrosomial structure which
} is required for easy classification of spermatids (and thus of
} stages of the seminiferous epithelium.)
} What should be avoided in any case is the presence of acids usually
} part of most recipes for fixatives, such as acetic acid and the like.
} If you use '10 percent formalin', you may experience some problems
} because you in fact never know the actual composition of such
} solution: it polymerizes on the shelf and becomes also quite acidic!
} You should prepare a solution of phosphate buffered (pH 7.2) 4 percent
} formaldehyde from paraformaldehyde powder on the same day (or the
} previous one) that you are sacrificing the animals (you should find
} recipes for making such solution in any practical handbook for
} microscopical technique ;-)).
}
} Fixation through immersion of the testes in the fixative may be
} almost acceptable for mice testes because they are small; this won't
} be the case for other species, even for the rat. You may try to make
} small (with caution!) incisions in the albuginea with a very sharp
} razor blade when the testes have stayed for at least an hour in the
} fixative, in order to improve penetration of the formaldehyde.
} Another trick to improve diffusion and fixation is to add from 0.5 to
} 1.0 percent (in volume) of DMSO (dimethylsulfoxide) to the fixing
} fluid (best is to combine both...)
}
} BUT,LAST and PROBABLY _MOST_ IMPORTANT: you should realize that
} formaldehyde fixation, i.e. crosslinking, proceeds exceedingly
} slowly! Therefore, tissues should stay in the fixative at least
} two weeks (one year or more won't harm!) before being further
} processed (two weeks probably is a conservative estimate). Assessment
} of pathological tissues fixed for only a few hours in NBF may
} frequently be required for practical reasons, but don't expect such
} methods to result in nice preservation of testis tissue, especially
} if you need estimating quality of spermatogenesis!
}
} You also may try to enhance fixation - and shorten the duration
} required for fixation - by performing it in the microwave oven: several
} cycles of no more than one minute at a time (perhaps 10 times, tissue
} temperatures exceeding 50 degrees celsius should be avoided), if
} you are indeed in a hurry and can't wait several weeks for
} fixation to proceed at its usual pace. If you try this, don't forget
} to place a water load (a becher container with about 750-1000 ml
} of water) besides your fixative containing flask in the oven.
}
} That's all what I can think of. HTH
} Good luck
} John
}
}
} ***********************************************************
} * Jean C. Desclin (John), Associate Prof. of Histology *
} * Laboratory of Histology - Faculty of Medicine *
} * Brussels Free University (U.L.B.) *
} * e-mail: desclinj-at-ulb.ac.be (internet) *
} * snail mail: route de Lennik 808 *
} * B - 1070 Brussels Belgium *
} ***********************************************************
}




From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Tue, 7 Mar 1995 11:12:26 -0600 (CST)
Subject: Re: Epoxy Resins: Accelerator?

Contents Retrieved from Microscopy Listserver Archives
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Dennis Shubitowski {dennis-at-odin.morph.med.umich.edu}
In-Reply-To: {Pine.3.03.9503021552.B11478-b100000-at-odin}
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On Thu, 2 Mar 1995, Dennis Shubitowski wrote:

} One of the divisions of our lab teases apart sural nerve tissue that have
} been kept in a 1:1 mixture of epon:propylene oxide. The samples are very
} easy to tease when the mixture is very fresh. The problem is that samples
} became back-logged and were not able to be teased right away. A quick
} decision had to be made, and the samples were frozen at -70C until they
} could be teased.
}
} The nerve fibers need to be infiltrated to be teased but not polymerized
} or else they become too fragile. A problem is becoming evident that after a
} long period of storage, the nerve samples are being fully infiltrated
} and polymerized despite being frozen (which we thought would almost stop
} the polymerization process because it is largely dependant on heat).
} Realizing that hind-sight is 20/20, would it have been better to leave the
} accelerator out of the epoxy mixture? What affect would this have on the
} quality of the resin? I understand accelerator to act as a catalyst being
} a part of the polymerization reaction but not really consumed. Any
} opinions to offer?
}
} Thanks,
}
} Dennis
}
Why do you store the fibers in resin? Isn't teasing them very messy? I
teased sural nerve fibers when
working for a neuropathologist. I fixed them and stored in buffer.
Also I sometimes used collagenase, which made the teasing easier. } } }
}




From: Laurie Frederick :      frederick_laurie-at-vanlab.paprican.ca
Date: Tue, 7 Mar 1995 09:20:30 PDT
Subject: Re: Image Analysis Systems

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Message-Id: {MAILQUEUE-101.950307092030.384-at-vanlab.paprican.ca}
To: "Griffin, Robin" {rgriffin-at-eng.uab.edu} , microscopy-at-aaem.amc.anl.gov

Hello,
I have information on an "Imagist" image analysis system from
Princeton Gamma Tech (PGT) which seems to be finding quite a bit of
popularity. I have come across it several times in speaking to users
during my hunt for an EDX system.
Imagist runs on a sparc station.
PGT in New Jersey, USA , can be reached at (609) 924-7310.

I have not used this system I have only seen it in use at the university
here.
Goodluck,
Laurie


On 1 Mar, 1995 Robin Griffin wrote:
}
} I'm interested in finding out what the most commonly used image
analysis
} systems for light microscopy, materials applications. I am aware of
} home-built, Beuler, Leco, and Kontron/Ibas. What else is out there
and what
} is used most in both University and industrial settings?
}
} Thanks for your help.
}
}

______________________________________________________________________
Laurie Frederick, A.SC.T. PAPRICAN
Corrosion Control Group 3800 Wesbrook Mall
The Pulp and Paper Research Vancouver, B.C.
Institute of Canada Canada V6S 2L9

Email: frederick_laurie-at-vanlab.paprican.ca
Tel: 604-222-3200 Fax: 604-222-3207




From: gwerdos-at-gnv.ifas.ufl.edu (Greg Erdos ICBR EM Core Lab)
Date: Tue, 07 Mar 1995 14:21:43 -0500 (EST)
Subject: Negative scanner

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We are considering purchasing a scanner for TEM negatives.

1. Is this a good idea?

2. Is there a consensus on which one to buy?

Any and all input appreciated.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv,ifas.ufl.edu
Gainesville, FL 32611





From: meh-at-aretha.jax.org (Margaret Hogan)
Date: Tue, 07 Mar 1995 15:38:40 -0500
Subject: job openning

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Histology - Biomedical Technologist/ Senior Biomedical Technologist

A regular full time position is open in The Jackson Laboratory Biological
Imaging Department - Histology Laboratory. The Jackson Laboratory is a
non-profit independent laboratory founded in 1929 on the premise that the
causes of cancer and other diseases could be discovered through
Mammalian genetic research. The Laboratory specializes in mammalian
genetics using inbred laboratory mice as model systems to study health
problems such as cancer diabetes, anemia, heart disease and aging.
Located on a large island in the gulf of Maine and surrounded by Acadia
National Park, The Jackson Laboratory is currently undergoing a major
expansion of its scientific staff and its research facilities.

Applicants with a Bachelors degree and two years related
laboratory experience working with Murine specimens preferred. The
position includes routine histological techniques ie paraffin embedding,
single and serial sectioning and cryotomy in conjunction with
Immunohistochemistry techniques, staining using heavy metals as well as
other special stains.

The successful candidate must be a self starter, pay attention to detail
and be able to work independently with little supervision.
This individual will be responsible for providing services in support of
numerous diverse research projects, must interact well with multiple
users and work productively in a team environment. The position includes
opportunities for advancement.

Salary range is mid to high $20,000 plus benefits and is negotiable
depending on level of experience.

Interested applicants should send CV to:

Joanne Bradt
Employment Specialist
The Jackson Laboratory
600 Main Street
Bar Harbor Maine 04609
(207) 288-3371 ext. 1281
(207) 288-3371 ext. 1082 FAX
jcb-at-aretha.jax.org





From: tivol-at-tethys.ph.albany.edu
Date: Tue, 07 Mar 1995 16:08:09 EST
Subject: Re: Negative scanner

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Dear Greg,
There are at least two types of scanner to consider depending on your
application. For scanning images of the usual type with many gray levels, a
CCD array will give excellent results and is very fast; however, for quantita-
ting ED patterns, nothing beats a scanner with a small illuminating spot. The
problem with a CCD array for ED is that the highest intensities are in areas
with very low transmission surrounded by areas with high transmission, so in-
ternal reflections in the CCD lens, etc. will give erronious values for the
intensities. Any of the high-resolution CCD arrays will work for images, since
the changes in contrast are not so abrupt. Perkin-Elmer and Optronics are two
names for small-illuminating-spot scanners, and doubtless there are others.
We had an Eikonix (linear CCD array) at one time, but were not happy with it.
Good luck.
Yours,
Bill Tivol




From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Tue, 7 Mar 1995 16:42:53 -500
Subject: Polaroid Films

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Here's a little information that I just received from polaroid
that should be of interest to the microscopy community. I'd be
interested in hearing any comments anyone (particularly any Polaroid
reps.) might care to share with the BBS.

Recently we have been noticing that the type 55 film we are
purchasing was arriving with a relatively short period before
expiration. We were questioning if our supplier might have trying to
unload older film on us. So I just called up Polaroid an asked them
what their lead time from manufacture to expiration date was, and I
was informed that for type 55 and type 665 (B&W P/N film) the
expiration lead time is only 9 months.

If you allow say 2-5 months from manufacture to shipping to
distributors to shipping to users (which seems very reasonable for
such commercial retail) this leaves only 4-7 months before
expiration! This short of an expiration date does not lend itself at
all to buying in larger quantities in order to obtain any sort of a
price break.

I do not know how much extension can be obtained by storage at say
4 C (May be someone out there does know). But we seem to start
having problems with film only a couple of months after the
expiration date even after cold storage for a few months.

You might want to consider this short time to expiration before
you go and stock up on polaroid film.


Richard E. Edelmann
Electron Microscopy Facility Supervisor
Miami University, Oxford, OH 45056
Ph: 513-529-5712
E-mail: edelmare-at-muohio.edu




From: John E. Johnson, Jr. :      76055.2216-at-compuserve.com
Date: 07 Mar 95 17:05:15 EST
Subject: Referees for Microscopy Research and Technique

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Although the journal Microscopy Research and Technique has a large editorial
board, we cannot cover every research area that involves microscopy. Therefore,
I would like to invite those of you who are interested in serving as ad hoc
referees for articles submitted to the journal to send, by e-mail, your name,
mailing (regular mail) address, phone number, fax number, and research interests
using key words such as biology, materials science, pathology, microanalysis,
stereology, magnetic domain, ceramics, immunocytochemistry, kidney, etc.

John E. Johnson, Jr., Ph.D.
Editor-in-Chief, Microscopy Research and Technique






From: gwerdos
Date: Tuesday, March 07, 1995 2:21PM
Subject: Negative scanner

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We are considering purchasing a scanner for TEM negatives.

1. Is this a good idea?

2. Is there a consensus on which one to buy?

Any and all input appreciated.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv,ifas.ufl.edu
Gainesville, FL 32611





From: charlesworth.jon-at-mayo.EDU (Jon Charlesworth)
Date: Tue, 7 Mar 1995 16:10:54 +0200
Subject: Tissue Processors

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Ed,
We have had 3 different tissue processors in the past 6 years, 3
LKB's, one Lynx, and 3 RMC's model 4189. LKB no longer manufactures a
system so I will forego 'ripping' their machine. We purchased a Lynx
system about four years ago. We found the same problem that W.L. Steffens
had with the QC of the expendibles. We lost tissue due to improper sealing
of the specimen chambers. Also the side 'openings' are relatively large
and we therefore do not run tissue smaller than 0.5 mm3 in these holders.
Extreme care must be used when removing tissue from the holders because
tissue has a tendency to adhere to the lid of the holder making it possible
to mix tissues when using the multiwell chambers. The unit itself though
has been extremely reliable.
About 10 months ago we purchased 3 RMC processors. These have
proven not to be as reliable as the Lynx (we have had temperature and some
programming problems). We therefore have had the opportunity to test RMC's
service department which to date has been able to get us up and running
with minimal down time. The big advantage of the RMC units are the
specimen chambers which seal better and hold smaller pieces of tissue.
Currently we use only the RMC units for tissue processing
(approximately 3000 samples annually). The Lynx system has been modified
and is used 3-4 days (and evenings) a week to immunolabel grids for our IEM
program. Hope this helps.

{jon charlesworth}
Electron Microscopy Facility
Mayo Clinic







From: gwerdos
Date: Tuesday, March 07, 1995 2:21PM
Subject: Negative scanner

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We are considering purchasing a scanner for TEM negatives.

1. Is this a good idea?

2. Is there a consensus on which one to buy?

Any and all input appreciated.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv,ifas.ufl.edu
Gainesville, FL 32611





From: fskarl-at-goodyear.com (Frank Karl)
Date: Wed, 8 Mar 1995 08:19:08 -0500
Subject: Long term storage of Polaroid film

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} noticing that the type 55 film ... with a relatively short period before
} expiration
}
} But we seem to start
} having problems with film only a couple of months after the
} expiration date even after cold storage for a few months.

Please forgive the above condenstation :-}

For the last 15 years we have stored Polaroid type 55 film in a freezer
without harm. This appears to negate the expiration date. We usually
allow the film to thaw out completely before opening the sealed package,
but sometime this process is rushed a bit and then, more often than not we
have problems.


Frank Karl fskarl-at-goodyear.com
Goodyear Tire & Rubber Co. Voice 216.796.7818
Analytical Services - Dept 415B Fax 216.796.3304
142 Goodyear Blvd
Akron, OH 44305
U.S.A.









From: Michael Boucher :      BOUCHER-at-tcrca.usbm.gov
Date: Wed, 8 Mar 1995 09:12:42 CST
Subject: Re: Polaroid Films

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We have found that to be the situation for years, but we order it by
the case and refrigerate it (not freezing). It seems to be good for
at least a year past expiration and we have had some film that was
several years old still work. I suspect that there could be some
degradation, but for our routine SEM and light microscopy, it hasn't
been a problem. Maybe Polaroid has done some testing?

*****************************************************************
Michael L. Boucher Sr. Boucher-at-TCRCA.USBM.GOV
Geology-Mineralogy/Chemistry Labs Ph 612-725-4614
Twin Cities Research Center Fax 612-725-4527
U.S. Bureau of Mines Center 725-4500
Department of Interior
5629 Minnehaha Avenue South
Minneapolis, MN 55417-3099
U.S.A.
*****************************************************************





From: chswartz-at-MIT.EDU
Date: Wed, 08 Mar 1995 11:06:43 EST
Subject: ultrathinsectioning material containing quartz

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I am going to be attempting to thin section sediment samples containing
quartz and want the thin sections to be approx. 50-70nm in thickness for TEM
analysis. I have to buy a diamond knife and would much appreciate input on the
blade angle to choose. A representative at Polysciences told me I should go to
a 55 degree knife instead of a 45, but I was wondering if I would be sacrificing
much of the ability to cut sections down to 50nm. The quartz grains are approx.
200 um in diameter. Is there a particular manufacturer I should get the knife
from? What is an appropriate cutting speed? Can I expect the knife to last
only a VERY short time? I would appreciate any suggestions and advice. My
address is:
chswartz-at-MIT.edu

THANKS




From: jerry-at-biochem.dental.upenn.edu
Date: Wed, 8 Mar 1995 12:03:50 -0500
Subject: Carbon Putty

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Does anyone on the listserver know where (prefereably in the U.S.)
that I can get Carbon Putty. I'm down to the last of what I had that
someone picked up at a conference in Germany a few years ago. It is
basically a mixture of some kind of soft wax and carbon and is a very good
specimen mounting material since there is no drying or out-gassing. Ted
Pella doesn't seem to have it so maybe someone out there knows about this
product and can save me a hunt.

Thanks---Jerry





From: ard-at-atom.ansto.gov.au (Arthur Day)
Date: Thu, 9 Mar 1995 04:22:35 +1100
Subject: Re: Polaroid Films

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} I do not know how much extension can be obtained by storage at say
} 4 C (May be someone out there does know). But we seem to start
} having problems with film only a couple of months after the
} expiration date even after cold storage for a few months.
}
} Richard E. Edelmann

We are just finishing our last few boxes of September 1994 Type 55 film
(stored in a refrigerator). It still seems to be OK.


Arthur Day, Electron Microscopy Group
Ansto Advanced Materials Program Phone: 61-2-717-3457
PMB 1, Menai (Sydney), NSW, 2234 Fax: 61-2-543-7179
Australia
Email: ard-at-atom.ansto.gov.au







From: Eric Kokko :      kokko-at-EM.AGR.CA
Date: Wed, 08 Mar 1995 15:30:52 -0500
Subject: Tracor 8502 users, help: Image convert to tiff

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X-Mailer: Novell GroupWise 4.1


------------------- tn8502.asc follows --------------------
Tracor 8502 users: Help with image convert to tiff

We operate a Tracor Northern (Noran) 8502 Image Analyzer and have
been trying to export images to a VAX via KERMIT with no success.
Has anyone done this sort of image file conversion and transfer
successfully?

Background: The Tracor software can convert an image file from
TN's format (.IMG) to a .TIF file (type 4, version 42). We can
successfully transfer this TIFF image file, via KERMIT, to or VAX
(ie. the file appears and has the appropriate size). When we try
to open this TIFF image (using a PC on our LAN), we always fail
regardless of the software we have employed (e.g. with Photoshop,
HiJack Pro).

If you have any ideas, please contact. I have lots of additional
contextual information to add to this. Thank you!

Eric Kokko
Electron Microscopy and Image Analysis
Agriculture and Agri-Food Canada
Lethbridge Research Centre
P.O. Box 3000,
Lethbridge, Alberta
CANADA T1J 4B1

Phone 403-327-4591 (Voice 367)
FAX 403-382-3156
INTERNET kokko-at-em.agr.ca





From: jerry-at-biochem.dental.upenn.edu
Date: Wed, 8 Mar 1995 15:47:44 -0500
Subject: Carbon Putty

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To those interested in Carbon Putty,

Someone has just given me all you could ever want to know about this
product. It is available from:
Bal-Tec Products, Inc.
984 Southford Road
P.O. Box 1221
Middlebury, CT 06762; (800) 875-3713, FAX (203) 598-3658

It is called "Leit-C-Plast; Cat. # B 801014075
Current price is $70.00 for 15g

Other properties:
-high electric conductivity
-long-life plasticity
-high vacuum resistance
-sufficient adhesive power
-low sample contamination
-no disturbing ED-x-ray lines
-Resistance R~100 kOhm mm2/m
Thanks for saving me the hunt---Jerry





From: M. ROOKS :      rooks-at-nnf.cornell.edu
Date: Wed, 08 Mar 1995 17:59:37 EST
Subject: carbon putty

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"Mung II" is not carbon putty, but is very much like it: electrically
conducting, a good thermal conductor, and very low vapor pressure.
We use Mung in our ion mill, but in the SEMs most people prefer clips
or carbon tape. You can buy mung from

Comonwealth Scientific Corp.
500 Pendleton St.
Alexandria, VA 22314
703-548-0800

$160 for 5 gr.



_______________________________________________________________
Michael Rooks 607-255-2329 voice
National Nanofabrication Facility 607-255-8601 fax
at Cornell University rooks-at-nnf.cornell.edu
Knight Laboratory
Ithaca, NY 14853 USA

__ __ __ __ ________ National
____ __ ____ __ __ Nanofabrication
__ ____ __ ____ _____ Facility
__ __ __ __ __ Cornell University

_______________________________________________________________






_______________________________________________________________
Michael Rooks 607-255-2329 voice
National Nanofabrication Facility 607-255-8601 fax
at Cornell University rooks-at-nnf.cornell.edu
Knight Laboratory
Ithaca, NY 14853 USA

__ __ __ __ ________ National
____ __ ____ __ __ Nanofabrication
__ ____ __ ____ _____ Facility
__ __ __ __ __ Cornell University

_______________________________________________________________








From: Charles A. Garber
Date: Thu, 09 Mar 1995 01:36:29 EST
Subject: "Carbon putty"

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To: Jerry-at-biochem.dental.upenn.edu

SPI Supplies

RE: Question about "carbon putty"

You are asking about a product called "Leit-C-Plast", which is found on
page 46 of the current SPI Supplies "SourceBook" as SPI #5057-AB. It is a
highly conductive adhesive plastic and is designed for mounting large
specimengo a very long way. I hope
this information will be useful to you.


Charles A. Garber, Ph. D.
PRESIDENT
SPI SUPPLIES
PO BOX 656
West Chester, PA 19380

Ph: (800) 242-4SPI
FAX: (610) 436-5755





From: John Millar :      jjmill-at-RMIT.EDU.AU
Date: Thu, 9 Mar 1995 10:38:46 EST-10
Subject: Re: Polaroid Films

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"Richard E. Edelmann" {EDELMARE-at-CASMAIL.MUOHIO.EDU}

Richard Edelmann wrote " I'd be
} interested in hearing any comments anyone (particularly any Polaroid
} reps.) might care to share with the BBS.
}
} Recently we have been noticing that the type 55 film we are
} purchasing was arriving with a relatively short period before
} expiration. We were questioning if our supplier might have trying to
} unload older film on us. So I just called up Polaroid an asked them
} what their lead time from manufacture to expiration date was, and I
} was informed that for type 55 and type 665 (B&W P/N film) the
} expiration lead time is only 9 months. "
}
I have used Polaroid 665,667,55 for nearly 20 years with no problems
of any continuing significance (except maybe cost !!). We have always
purchased in bulk (large boxes of 50 boxes) and stored the material
in a standard refrigerator, not freezing it. Usual practice would be
to allow the boxes to equilibrate at room temperature before use but
sometimes usage rate prevented this. The sensitivity does appear to
change with temperature, but we have nver bothereded to investigate
in detail. In the usual situation, we have not expereinced any
problems and have both negs and prints which are old and still very
good quality.
Cleanliness of the holder and specially the rollers is critical to
performance and they are cleaned after EVERY cartridge is exhausted
and before the next one is in place.
cheers
jjm
Professor John J. Millar
Head, Department of Applied Physics
Electron Microscope Unit
RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001
+613 660 2602 fax 613 660 3837
email jjmill-at-rmit.edu.au




From: Charles A. Garber
Date: Thu, 09 Mar 1995 01:36:53 EST
Subject: Thin sectioning of quartz particles

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To: chswartz-at-MIT.edu

SPI Supplies
West Chester, PA 19380 USA

RE: Ultrathin sectioning material containing quartz

We have had some amount of experience in our own laboratory with the thin
sectioning of these kinds of samples for TEM characterization.

There are the obvious trade offs, that is, th that a) the price is much
cheaper and b) you are using the "normal" narrow angle (e.g. 45 deg.)
knives and therefore you don't have to worry about compression effects.

While the ability to cut sections is somewhat related to the experience of
the one doing the sectioning (and it is also an art), so long as you are
patient, you should be able to do it.

If you wanted to send us one of your samples, we could produce sections for
you, and essentially determine the exact conditions under which you too
could easily get sections. There would be a fee of course for this,
however, you would know for sure, one way or another, whether sections
could or could not be made of your material,and if they could, then you
would also know the exact cutting conditions.

Another important consideration would be the choice of embedding resins and
we would recommend for quartz particles our SPI # 2660 SPI-PON 812 resin
kit since this particular resin seems to be the most user friendly in terms
of polymerizing to a hardness for which sections can be made of the
embedding quartz particles.

You have also asked a question that touches on the expected longevity of
the diamond knife when cutting these kinds of materials. You sort of have
to think about it as an analog to the mileage expected from a pair of
tires. You can buy imported tires or you can buy domestic ones, however,
what really counts is how you drive your car. Be gentle, and the knife will
last longer. Be abusive, and it will have a shorter life time. Obviously,
cutting quartz particles is going to be "hard" on the knife. You can
extend the longevity be making the particles smaller before embedding. You
can get good sections faster, and therefore also less knife wear, by curing
the SPI-Pon 812 resin harder right away, putting less wear and tear on the
knife to get to that point.

I hope this information has been helpful to you. Let me know if you have
any other questions.

Charles A. Garber
SPI Supplies
PO Box 656
West Chester, PA 19380 USA

Ph: (800) 242-4SPI
FAX: (610) 436-5755
e-mail: cgarber-at-cerfnet.com





From: jouneau-at-cime.epfl.ch (Pierre-Henri Jouneau)
Date: Thu, 9 Mar 1995 15:51:13 +0100
Subject: TRINOCULAR JOINT MEETING ON ELECTRON MICROSCOPIES

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***************************************************************************

ANNOUNCEMENT

Trinocular Joint Meeting on Electron Microscopies

Lausanne, June 26-30, 1995

***************************************************************************

The Swiss Society of Optics and Electron Microscopy, the French Society
of Electron Microscopy and the Belgian Society of Microscopy have decided
to hold a joint meeting in 1995. This conference will be held at the
"Ecole Polytechnique Federale de Lausanne" (EPFL), Lausanne, Switzerland,
from June 26 to 30, 1995. It will include two tutorial days and a three-days
conference which will be mainly focused on the recent progresses in TEM and
scanning probe microscopies for materials science and biological fields.


********** SCIENTIFIC PROGRAMME **********

Monday 26 and Tuesday 27
TUTORIALS, with practical training, for groups of 6 to 20 people.

- Cryo-microscopy of vitrified specimens: theory and practice
(Jacques Dubochet)
- Practical aspects of the methodology of image analysis in oncology
(Ricardo Laurini)
- TEM image simulation of crystalline materials (Pierre Stadelmann)
- Electron holography (Conradin Beeli)
- Analytical microscopy with a field emission TEM (Klaus Leifer)
- TEM sample preparation in materials science (Daniele Laub)
- Applications of large angle convergent beam electron diffraction
techniques (LACBED) (Jean-Paul Morniroli)

Evening Tuesday 27
Welcome ceremony and conference opening lecture.

"Perception du monde exterieur par les systemes vivants"
by Yves de Ribaupierre, University of Lausanne.

Wednesday 28, Thursday 29 and Friday 30 - Conference

**** PHYSICS-MATERIALS-BIOLOGY-PATHOLOGY JOINT SYMPOSIA ****

- New microscopies: STM, AFM, SNOM, confocal, ultra-sound, ...
(A. Engel, D. Pohl)

- Energy filtered images and electron spectroscopy
(C. Colliex, D. Bazett-Jones)

- Life in extreme environment (F. Gaill)

**** BIOLOGY-PATHOLOGY SYMPOSIA ****

- Fertilization and early embryogenesis in mammals
(J. E. Flechon)

- Freeze substitution, microscopy of frozen hydrated samples
(J. Dubochet, S. Fakan)

- Image analysis and cancer diagnosis (R. Laurini)

- High-resolution electron microscopy of biological specimens
(E. Delain, M. Muller)

- Cytoskeleton (G. Gabbiani)

- New applications of scanning probe microscopies
(M. Robert-Nicoud)

**** PHYSICS-MATERIALS SYMPOSIA ****

- Electron holography (C. Beeli, D. van Dyck)

- New progress on CBED/LACBED (J. P. Morniroli, J. Steeds)

- High-Resolution microscopy of aperiodic structures
(J. van Landuyt, P.Stadelmann)

- New applications of analytical microscopy: filtered images, EDS, EELS
(C. Colliex, C. Humphreys)

- New applications of scanning probe microscopies (D. Pohl, D. Courjon)

**** Commercial exibition ****

A substantial commercial exhibition of scientific equipment will also be
held during the conference. For information, please contact G. Peter,
EPFL-CIME CH 1015 Lausanne
Fax: +41 [21] 693 44 01, email: peter-at-cime.epfl.ch


********** REGISTRATION **********

The symposia will consist essentially of a small number of lectures (mainly
invited, with some selected from the conference abstracts) and of poster
sessions. The presentations may be given in English, or in any languages
of the three national societies. However, certain parts of the presentation
should be in English, at least abstracts, figure captions, transparencies...
Discussion sessions will be devoted to a few hot points emerging from the
poster presentations.

The registration fees for the conference, including attendance at the
symposia and the abstract booklet, are 250 CHF (non-members), 200 CHF
(members) and 100 CHF (students and lab technicians). The registration fees
for the tutorial days will be communicated in the final circular. A maximum
of 100 CHF/course is expected. A few grants have been founded by the
national societies in order to encourage the participation of young
scientists and technicians


********** GENERAL INFORMATION **********

The conference will be held at the Ecole Polytechnique Federale de Lausanne
(EPFL), located approximately 4 km from the city centre, near (500 m) the
idyllic scenery of lake Leman. Hotel rooms in different categories (from
"Formule 1" to ****) have been reserved by the tourist office for the
organization committee, with prices ranging from 25 (F1, 3 persons per room)
to 230 CHF. A final hotel booking form will be included with the registration
form.

Excursions for participants and accompanying persons are planned: e.g. a
tour to the beautiful region of "la Gruyere" famous for its cheese, to a wine
cellar on the coast of lake Leman !! ... These excursions will be organized
in small groups of 20 to 30 persons according to their preferences.


********** ORGANIZING COMMITTEES **********

Honor committee:
Walter Bollmann, Alain Gautier, Eduard Kellenberger, Bernard Vittoz

International Scientific Committee:
M. Deschuyteneer (SmithKline Beecham, B), J. Dubochet (Uni. Lausanne, CH),
A. Engel (Uni. Basel, CH), J. Gunter (Uni. Zurich, CH),
D. Hernandez-Verdun (Inst. Jacques Monod, F), J. Lecomte-Beckers
(Uni. Liege, B), J.-P. Morniroli (Uni. Lille, F), R. Portier (ENSCP, F),
M. Praet (Uni. Gent, B), D. Schryvers (Uni. Antwerpen, B), P. Stadelmann
(EPFL Lausanne, CH), D. Thomas (Uni. Rennes, F)

Local Scientific Committee:
M. Campiche (Uni. Lausanne), J. Dubochet (Uni. Lausanne),
S. Fakan (Uni. Lausanne), R. Gotthardt (EPFL Lausanne),
R. Laurini (Uni. Lausanne), P. Stadelmann (EPFL Lausanne)

Local Organizing Committee:
P.A. Buffat (Chairman), R. Rouquier (Secretary),
C. Beeli, F. Bobard, B. Garoni, P.-H. Jouneau, D. Laub, G. Peter,
B. Senior, P. Stadelmann


***************************************************************************

For additional information and registration details, please contact
as soon as possible:

Mrs Ruth Rouquier, Congres Trinoculaire,
CIME-EPFL, CH 1015 Lausanne, Switzerland
Fax: (021) 693 4401, Tel: (021) 693 4405
email: ruth.rouquier-at-cime.epfl.ch, http://cimewww.epfl.ch

***************************************************************************



Pierre-Henri Jouneau
Ecole Polytechnique Federale de Lausane
CIME-EPFL, CH-1015 Lausanne
tel: +41 (21) 693 44 37 fax:+41 (21) 693 44 01






From: Giles John E Jr on Thu, Mar 9, 1995 10:07 AM
Date: 9 Mar 1995 10:21:34 U
Subject: Digital Images

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I am looking for information about software to read .bmp or TIFF files using a
Mac. We have a Targa+ video capture board to capture images from the SEM onto
a 486-66 PC. I would like to be able to import them over e-mail to a Mac
Quadra 650 and paste into a Word document. Word will import the image, but
there seem to be a decrease in resolution and the file size is reduced from
650K to 375K when it is converted to the Mac.
I would like to here from other users who are capturing images as we are just
getting the system set up.
Thanks,

John Giles
Honeywell Space Systems
jegiles-at-space.honeywell.com
(813)539-2270 phone
(813)539-3630 Fax





From: ychen-at-macc.wisc.edu
Date: Thu, 9 Mar 1995 12:55:21 -0600
Subject: Re: Polaroid Films

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Message-Id: {25030912503075-at-vms2.macc.wisc.edu}

} I do not know how much extension can be obtained by storage at say
} 4 C (May be someone out there does know). But we seem to start
} having problems with film only a couple of months after the
} expiration date even after cold storage for a few months.
}
} Richard E. Edelmann

I had a problem of inside sticky #52 Polaroid film, which will expire on
Sept. 1995, so that run out of our film. I found a box of old #52 film
which expired on Aug. 1992 left in a drew (at room temperature) and tried
to use it. To my surprise, it worked fine.
For my experiences, the problem of Polaroid does not really related to the
expiration date. Instead, I found a lot of problems are caused by storage.
I have used a lot of good expired films and a lot of fresh films as well
for 5 years.

Ya Chen
Integrated Microscopy Resource
Madison, WI 53706
USA
Email: ychen-at-macc.wisc.edu






From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Thu, 9 Mar 1995 08:33:53 -0700
Subject: Re: Fujix Pictrography 3000 Printer

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Message-Id: {v01510101ab84cec55d80-at-[129.82.126.28]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

William Dentler wrote:

} We are in the process of purchasing a new printer and are looking at the
} Tektronix Phaser II SDX, Kodak ColorEase PS, and Kodak 8600. Samples
} provided by
} Kodak and Tektronix look great. I have heard that the Mitsubishi S3600-32U
} is a
} great printer but cannot get any useful information, prices, or samples from
} Mitsubishi or local dealers. I am curious about the Fujix 3000. Could you let
} me know how or where to contact Fujix, since I have never heard of them.

You can contact Ron Saltzman, at Fujix Electronic Imaging Group at
800-736-3600 ext. 8282 (voice mail). If he's no longer with that group,
you might try the general numbers, 914-789-8100 or 800755-3854, to get to a
sales rep. They also advertise in some of the photo lab trade magazines.

Good luck. They are really worth a look.


John chandler-at-lamar.ColoState.EDU Fort Collins CO






From: jerry-at-biochem.dental.upenn.edu
Date: Thu, 9 Mar 1995 10:53:34 -0500
Subject: Carbon Putty

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Thanks to those who have helped me locate sources where I could find
a material matching the description I gave for what I called "carbon putty."

Since posting the first source given to me (Bal-Tec) yesterday, I
have received a few more. So, for those other subscribers who asked me to
post any info. I got, here are the other sources.

SPI Supplies/P.O. Box 656/West Chester, PA 19380/(800)242-4SPI/
Cat.# SPI 5057/$63.75 15g

Marivac LTD./Hailfax, N.S./Canada/(800)565-5821/Cat.# AS508-3/
$66.50 Canadian 15g (Approx. $50.00 American)

SGL-Carbon/Niagara Falls, N.Y./P.O.Box 667/(716)236-2859/
This company makes carbon epoxy material that sets-up for permanent
attachments. It is not a soft putty after it sets but it is highly
conductive and may be of some use for other purposes.

Thanks for all the help --- Jerry





From: jyoung-at-inforamp.net (James Young)
Date: Thu, 9 Mar 1995 18:28:32 +0500
Subject: Info on Agr Sta Closings

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I was talking to Frank Skelton. It seems Agr Cda Van is closing. Any other
closings or layoffs?
Interested only.

JIM Young





From: images1-at-biosci.mbp.missouri.edu (Michael Stanley)
Date: Thu, 9 Mar 1995 18:01:19 -0600
Subject: zamboni fix

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Hello All,
I have a user who was told to try Zamboni Fix on their tissue.
Could some kind soul tell us what it is and how to make it. I know what
and Zamboni is in relation to ice rinks and I can even drive one (it's a
real hoot!!) but I've never heard of the fix...
TIA,

C. Michael Stanley, Ph.D.
Coordinator, Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2 Tucker Hall
University of Missouri-Columbia
Columbia, MO. 65211
(314) 882-4895
fax= 314-882-0123






From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Fri, 10 Mar 1995 11:21:44 +1100
Subject: Tissue processors

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Message-Id: {199503092314.MAA28868-at-arwen.otago.ac.nz}
X-Sender: st004718-at-brandywine.otago.ac.nz
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Ed Calomeni wrote:
} I am in the info gathering stage of purschasing a tissue processor for EM
} samples. } I have info on the Reichert-Lynx unit and the one made by RMC. How
} do you like } these units? Comments on advantages and disadvantages are
} welcome.

} Thanks in advance

} Ed Calomeni
} Medical College of Ohio
} Toledo, OH

We have a Lynx tissue processor which we are now very happy with. There
were a few teething problems when it was first installed (the main control
board and the motor control unit had to be replaced) but these problems I
believe have now been sorted out at the factory and shouldn't apply to new
instruments now - it was some three years ago when we bought our one.
The only other problem we had was with voltage spikes from the mains. This
problem was eliminated by putting an isolating transformer between the
mains and the processor.
We have not experienced any problems with the samples mixing upon opening
up the vials (someone -sorry I forget who- mentioned this in an earlier
reply).
Infiltration of resin seems to be fine, something I was a little sceptical
of initially, given the small holes in some of the basket types. There is a
good choice of different specimen baskets anyway and I have always found
one suitable for whatever specimens I happen to be processing.
Hope this is helpful for making your decision.
Regards,
RE



Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254






From: pmarkiew-at-alchemy.chem.utoronto.ca (Peter Markiewicz)
Date: Wed, 08 Mar 1995 15:50:25 -0500
Subject: AFM deconvolution program

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Message-Id: {199503092054.PAA03408-at-alchemy.chem.utoronto.ca}
X-Sender: pmarkiew-at-alchemy.chem.utoronto.ca (Unverified)
X-Mailer: Windows Eudora Version 1.4.4
Mime-Version: 1.0
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Fellow Microscopists,

This is a general announcement which should be of interest to atomic force
microscopists.

We are making the latest version of our deconvolution program MIDAS95
available for free on the Internet. The program is designed to work under
Windows on Nanoscope files. It is being released as a beta version, meaning
the program lacks a certain polish. The program works properly for both the
Nano II and III, although testing on the latter system has been limited.

Through deconvolution, one can account for the volume occupied by the tip in
the AFM images obtained. By eliminating the tip effect, the result is often
a truer representation of the sample topography. Deconvolution also allows
for the in situ measurement of the tip geometry. This 3D data file of the
tip can be used as a check of the tip's integrity or for improvement of
other AFM images. Further details are given in the README.TXT file.

The program can be accessed by the following:
ftp surfturf.chem.utoronto.ca
login: anonymous
password: yourname-at-location
Please use your E-mail address for the password. We wish to keep a record
of the users of this program and notify them of any problems or updates.

The public directory has three files of interest here. One is the
README.TXT file if you want to know what is in MIDAS.ZIP without having to
download it. MIDAS.ZIP contains several files, including some AFM files to
see how deconvolution is done and some TIFF files for viewing. Fan-xing Wei
and Dr. Dan Thomas of the University of Guelph have modified our program so
that it works for Burleigh Instruments. This program is available in BURL.ZIP.

If you have any difficulty, please be patient as our server allows only one
user at a time and tends to lock up when doing calculations.

Thank you,

Peter Markiewicz





From: waheeschen-at-dow.com (Bill Heeschen (517)636-4005 PMRC/ASL)
Date: Thu, 9 Mar 1995 16:19:57 -0500
Subject: re: Digital Images

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John

The best way to get bitmap and/or TIFF images into your Mac is with the NIH
Image software (via ftp: anonymous-at-zippy.nimh.nih.gov directory /pub/nih-
image). It can handle either format, but you will probably have to go through
the IMPORT diaolog, not the OPEN dialog. Look at the documentation (also on
Zippy) to guide you through. As long as there is a full valid bitmap in the
image file, NIH Image can import the image and allow you to save it as a useful
file. We have done a lot of image file format trouble-shooting for "standard"
formats written by multiple vendors on multiple platforms around here using
that capability.

A plug here: NIH Image has a nearly infinite performance to price ratio - it
is free once you are on the internet. It also outperforms a whole lot of
commercial stuff on any computer platform up to about the $2000 price point and
the support system is unbelievably good, considering the annual maintenance non-
fee. 8-).

Two hurdles are: getting the file onto your Mac (which it sounds like you are
already doing) then getting a good image into Word. I do the latter by pasting
the image from NIH Image into Word, then shift-shrinking the image to about
50%. The original paste goes in at 72 dpi, the shift-shrink gives the final
image resolution of 144 dpi which, with a decent gray-scale printer, gives you
very acceptable print quality. By the way, I __STRONGLY__ advise against using
the image editor in Word. I have found it unreliable in addition to the fact
that it reduces your grayscale image to 16 levels. This could explain the drop
in file size (the editor converts the 8-bit image to 4-bit). A similar result
would happen if you started with a 16-bit TIFF and wound up with 8-bit.

Hope this helps.


Bill
==========================
Bill Heeschen/Analyt. Sci.- Mat. Char. -----
1897-F Building / The Dow Chemical Co. --- ---
Midland, MI 48667 U.S.A. --- D O W ---
phone: (517)636-4005 fax: (517)636-5453 --- ---
Email: waheeschen-at-dow.com -----
========================== R




From: {chswartz-at-MIT.EDU}:ddn:wpafb
Date: 3-8-95 12:54pm
Subject: Re: ultrathinsectioning material containing quartz

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Message-Id: {9503092328.AA21558-at-riker.ml.wpafb.af.mil}
To: WALCKSD:ML:WPAFB
cc: Lloydpf:ML:WPAFB
Subj: ultrathinsectioning material containing quartz
In-Reply-To: Message from WALCKSD:ML:WPAFB of 3-8-95
-----------------------------------------------------------------------
Scott,

I would recommend that the knife of choice be a Diatome knife. The angle
would depend on how hard the sediment is. If the sediment is very hard, then
yes I too suggest a 55x knife. Using a knife with this angle still allows one
to obtain sections on the order of 70nm. If the sediment is not that hard,
then a 45x knife will work also. I generally use a 55x knife for all materials
that I cut except for polymers, where I use a 45x knife.

I hope this helps!

Pam


---------------------- Replied Message Body ----------------------
To: LLOYDPF
Subj: ultrathinsectioning material containing quartz
Forwarded: Message from {chswartz-at-MIT.EDU}:ddn:wpafb of 3-8-95
------------------------------------------------------------------



--------------------- Forwarded Message Body ---------------------
To: WALCKSD:ml:wpafb, SCHELTFJ:ml:wpafb
Subj: ultrathinsectioning material containing quartz
Orig-Author: {chswartz-at-MIT.EDU}:ddn:wpafb
-----------------------------------------------------------
I am going to be attempting to thin section sediment samples containing
quartz and want the thin sections to be approx. 50-70nm in thickness for TEM
analysis. I have to buy a diamond knife and would much appreciate input on the
blade angle to choose. A representative at Polysciences told me I should go to
a 55 degree knife instead of a 45, but I was wondering if I would be
sacrificing
much of the ability to cut sections down to 50nm. The quartz grains are
approx.
200 um in diameter. Is there a particular manufacturer I should get the knife
from? What is an appropriate cutting speed? Can I expect the knife to last
only a VERY short time? I would appreciate any suggestions and advice. My
address is:
chswartz-at-MIT.edu

THAN





From: Mike Gregory :      mgregory-at-pixie.udw.ac.za
Date: Fri, 10 Mar 1995 08:34:29 +0200 (SST)
Subject: Re: BDMA vs DMP-30 summary

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On Mon, 6 Mar 1995, Gib Ahlstrand wrote:


Hi Gib:

I'd appreciate a copy of the BDMA vs DMP-30 responses.

Regards

Mike Gregory

} In July and September of 1994, there was some disciussion of BDMA vs. DMP-30. I
} saved and consolidated 13 of those messages into a single document. If anyone is
} interested in getting a copy, send me your e-mail address and I will zip one out
} to you within a day or two.
}
} --
}
} Gib Ahlstrand, MMS Newsletter Editor
} Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
} 495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
} 612-625-9728 FAX, giba-at-puccini.crl.umn.edu
}
} "MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996
}
}




From: Doug Arrell :      ARRELL-at-jrc.nl
Date: Fri, 10 Mar 1995 08:33:09 GMT+0200
Subject: re: Digital Images

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} The best way to get bitmap and/or TIFF images into your Mac is with the NIH
} Image software (via ftp: anonymous-at-zippy.nimh.nih.gov directory /pub/nih-
} image). It can handle either format, but you will probably have to go through
} the IMPORT diaolog, not the OPEN dialog. Look at the documentation (also on
} Zippy) to guide you through. As long as there is a full valid bitmap in the
} image file, NIH Image can import the image and allow you to save it as a useful
} file.

As a follow-up, we use NIH Image for our image processing and also
import BMP and TIFF files from PC capture systems. NIH Image will
import TIFF directly using 'OPEN', and from our experience BMPs
should be IMPORTed using CUSTOM, setting OFFSET to 1760 and chsing
the relevant bitmap size.

I hope this is of use

Doug


+------------------------------------+
| Dr Douglas Arrell |
| Mechanical Performance and Joining |
| Institute for Advanced Materials |
| 1755 ZG Petten |
| Netherlands |
| {ARRELL-at-JRC.NL} |
| Tel. (+31) 2246 5287 |
| Fax (+31) 2246 1917 |
+------------------------------------+




From: epicier-at-univ-lyon1.fr (Thierry Epicier)
Date: Fri, 10 Mar 1995 08:39:58 +0100
Subject: S-SIMPLY update...

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A short message for users of (or people interested in) 'S-SIMPLY'
(SHRLI-SIMulation and diSPLaY of TEM & HRTEM images, for PCs) : the freeware
version, available on FTP.UNIV-LYON1.FR (/pub/dos/HRTEM) has been
significantly updated, and new features are available (graphical displays, a
"Make Interface" tool,...).
With best regards,

______________________________________________

Thierry EPICIER
GEMPPM-502, INSA de Lyon,
69621 VILLEURBANNE, France
tel : (33) 72 43 84 94 (83 85)
FAX : (33) 72 43 85 28
Email : EPICIER-at-CISMSUN.UNIV-LYON1.FR





From: jerry-at-biochem.dental.upenn.edu
Date: Fri, 10 Mar 1995 08:44:59 -0500
Subject: Carbon Putty correction

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Via: bham.ac.uk; Fri, 10 Mar 1995 09:08:17 +0000

To those interested in Carbon Putty,

Two days ago I posted a Cat.# and price for Leit-C-Plast from
Bal-Tec that was given to me incorrectly. The correct Cat.# is B801014077
and the cost is $40.00 for 15g. This is now the best price I've found.

Bal-Tec Products/984 Southford Rd./P.O.Box 1221/Middlebury CT/
(800)875-3713;Fx (203)598-3658

Hope this helps---Jerry





From: Marcel.Paques-at-2488PAS.urlnl.sprint.com
Date: Fri, 10 Mar 1995 10:19:00 -0500
Subject: Fuji Pictography 3000 printer

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I recently purchased a Pictography 3000 printer after a extensive
comparison of printer systems available on the market.
The printer is attached to the network (Token ring, novell) via a pc,
and is transparent configured: all pc's in the network can reach the
printer.
The printer is used for production of hard-copies of image files
generated by various techniques: CSLM, VEM, cryo-SEM, FESEM (+EDS
Noran SUN), TEM (Gatan SSC, Mac), SPM, IA (SEMPER).

We are quit satisfied.

E-mail: Marcel.Paques-at-2488PAS.URLNL.Sprint.Com
Unilever research Laboratory Vlaardingen
The Netherlands




From: A. Kent Christensen :      akc-at-umich.edu
Date: Fri, 10 Mar 1995 10:42:37 -0500 (EST)
Subject: Re: zamboni fix

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Zamboni fix is an EM version of the classical LM Bouin's fix.
Zamboni's original abstract didn't contain any details about how to make
it up. The details are in: Stefanini et al., 1967, Nature 216:173.

A. Kent Christensen
Department of Anatomy and Cell Biology
University of Michigan Medical School
{akc-at-umich.edu}

------------------------------------------

On Thu, 9 Mar 1995, Michael Stanley wrote:

} Hello All,
} I have a user who was told to try Zamboni Fix on their tissue.
} Could some kind soul tell us what it is and how to make it. I know what
} and Zamboni is in relation to ice rinks and I can even drive one (it's a
} real hoot!!) but I've never heard of the fix...
} TIA,
}
} C. Michael Stanley, Ph.D.
} Coordinator, Associate Director
} Molecular Cytology Core Facility
} Molecular Biology Program
} 2 Tucker Hall
} University of Missouri-Columbia
} Columbia, MO. 65211
} (314) 882-4895
} fax= 314-882-0123
}
}
}




From: Smith, Peter :      SMithP-at-agresearch.cri.nz
Date: Thu, 09 Mar 1995 14:14 +1200 (NZST)
Subject: [LM] -Immunocytochemistry method using c

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I have recently been shown a research report in BioTechniques, Vol 13, No
3. (1992) by Reed, J.A., Manahan, L.J., Park, C-S, and Brigati, D.JL
describing an immunocytochemical method based upon capillary action. This
was using the "MicroProbe" marketed by Fisher Scientific, Pittsburgh, PA.

My question is, has anyone used this system? If so what are its advantages
and/or disadvantages . Also could you please give me a contact address, FAX
number or E-Mail for Fisher Scientific.

Thanks in advance.




From: Beverly E Maleeff :      Beverly_E_Maleeff%notes-at-sb.com
Date: 10 Mar 95 10:09:30 ES
Subject: Re: zamboni fix

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Message-Id: {9503101815.AA2900-at-pho018.sb.com}
To: images1 {images1-at-biosci.mbp.missouri.edu} ,
microscopy
{microscopy-at-aaem.amc.anl.gov}

In response to this question:

Hello All,
I have a user who was told to try Zamboni Fix on their tissue.
Could some kind soul tell us what it is and how to make it. I know what
and Zamboni is in relation to ice rinks and I can even drive one (it's a
real hoot!!) but I've never heard of the fix...
TIA,

C. Michael Stanley, Ph.D.
Coordinator, Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2 Tucker Hall
University of Missouri-Columbia
Columbia, MO. 65211
(314) 882-4895
fax= 314-882-0123


I think that the fixative you're referring to is cited in this paper:
Zamboni, L. and DeMartino, C., 1967. Buffered picric acid-formaldehyde: A new,
rapid fixative
for electron microscopy.

We came across Zamboni's fix when we were perfusion-fixing rat testes a couple
of years ago.
However, it didn't preserve Leydig cell structure as well as some of the other
fixatives we tried.

Hope this info helps. (I want to hear more about driving a Zamboni!)

Bev Maleeff
SmithKline Beecham Pharmaceuticals
Toxicology-US, UE 0462
709 Swedeland Road
King of Prussia, PA 19406
610/270-7987
610/270-7202 fax
e-mail: maleeffbe-at-sb.com





From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Fri, 10 Mar 1995 17:12:16 -0500 (EST)
Subject: Meeting Announcement, Atlanta GA

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The 30th annual meeting of the SouthEastern Microscopy Society (SEMS) will be
held on May 17-19, 1995, at the Omni Hotel in Atlanta, GA. Scheduled invited
speakers and their titles are: 1) Nestor Zaluzec, Argonne National
Laboratory, Integrating Computers, Microscopy, and Microanalysis; 2) Phil
Russell, North Carolina State University, Instrumentation and Application of
Scanned Probe Microscopy; 3) Larry Peterson, GBI, Forensic Microscopy; 4)
Terence Mitchell, Los Alamos National Laboratories, Experience with Field
Emission on an SEM, STEM, and TEM; and 5) Jay Jerome, Bowman Gray School of
Medicine of Wake Forest University, Exploring Ultrastructure with
Quantitative 3-D Intermediate Voltage Electron Microscopy. Also on the
program are pre-meeting workshops and tours, contributed papers and posters,
the RUSKA student competition, and commercial exhibits. Social events are the
Wednesday night Exhibitor's Mixer and the Awards Banquet, which will be held
Thursday night at the Fernbank Museum of Natural History

For further
information, contact Janet H. Woodward, SEMS '95 Program Chair, at (912)-945-
3152, FAX (912)-945-3155.



Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: jyoung-at-inforamp.net (James Young)
Date: Sat, 11 Mar 1995 20:03:18 +0500
Subject: Your message.

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My goodness I got the message several times. Also the one I sent came back
to me several times. I guess the Government wants to make sure evereything
gets through.

Too bad about the cuts. Frank Skelton told me about Van Closing also I guess
the Farm got hit.

PCI is selling well particularly in the USA and some to Japan.

I have full internet access now. PPP with every application which is
Freeware and Shareware. All windows based and relatively easy to use.

Our site in the office is working under a private account, but we haven't
got the domain name yet. It will be NSCTOR. Anyway I will let you know
when it comes through.





From: Peter Goodhew :      goodhew-at-liverpool.ac.uk
Date: Mon, 13 Mar 1995 08:51:38 GMT
Subject: Re: Electron optics teaching

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David Cockayne asked:
}
} WITH REGARD TO THE QUESTION ABOUT TEACHING OPTICS, CAN ANYONE
ADVISE ME OF WHAT SOFTWARE PACKAGES ARE AVAILABLE FOR TEACHING
ELECTRON OPTICS?

At the same time a similar question was posted on the Microscopy
list (by someone different).

I would be delighted if anyone can update the following list of
teaching software (in the area of microscopy) known to me:

The Institute of Materials (UK) distribute:

The Transmission Electron Microscope by Goodhew
The Scanning Electron Microscope by Humphreys (John)
Electron Diffraction by Goodhew
The Stereographic Projection by Humphreys
Analysis in the Electron Microscope by Goodhew, Humphreys and
Cliff
X-ray Photoelectron Spectroscopy by Watts
X-ray Auger and Laser emission by Goodhew

These are all IBM PC DOS versions, available from Institute of
Materials, 1 Carlton House Terrace, London, SW1Y 5DB, fax
+44(0)171 839 2078
The MATTER project is currently working on greatly-improved
Windows and Mac versions of most of these topics: Completion of
the first two topics (TEM and SEM) is scheduled for the end of
1995. All the above are aimed firmly at teaching, not at research
applications.

There is also an SEM simulation available which originated in
Australia but which is supposed to be downloadable from Nestor
Zaluzec's Microscopy Bulletin Board. However we have consistently
failed to transfer it. Has anyone in the UK succeeded?

I also saw recently a very full simulation of a light microscope.
This is aimed at biologists and was written (and is distributed)
by Maurice Smith at: mol-at-molcol.demon.co.uk

If you can add to this list please reply to both lists [
microscopy and materials-l ]

Thanks

Peter

PS 120+ members of the materials list now!

------------------------------------------------------------------
-----
Professor Peter J Goodhew, Department of Materials Science &
Engineering
University of Liverpool
LIVERPOOL Fax (44) (0)151 794 4675
L69 3BX, UK Tel (44) (0)151 794 4665 (secretary
Debra)
------------------------------------------------------------------
-----
inter alia: Director of the MATTER project for educational
software
------------------------------------------------------------------
-----






From: Fangl-at-fpms.fpms.ac.be
Date: Mon, 13 Mar 1995 12:29:36 +0100
Subject: help

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Dear Sir: Would please give some information about mail list of microscopy.
L.FANG




From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Mon, 13 Mar 1995 10:30:19 -0600 (CST)
Subject: Midwest Society of Electron Microscopists

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Midwest Society of Electron Microscopists

STUDENT COMPETITION
TO BE HELD AT
THE UNIVERSITY OF WISCONSIN
WHITEWATER, WISCONSIN
Ambrose Health Center, Room 162
Friday, March 24, 1995
PROGRAM:
1:00 PM: Dr. Ursula K. Charaf, Senior Research Scientist, Johnson Wax:
BLOW IT UP! Applied Industrial Microscopy
1:45 PM: Student Presentations
3:00 PM: Dr. Robert Cardell, University of Cincinnatti Medical Center,
MSA/LAS Sponsored Presidential
Speaker: Modern
Microscopical Approaches to Biomedical Problems
4:00 PM: Reception and Award Presentations

If you are planning to come, telephone or E-mail registrations are
required by March 22.
If you are planning to present your research, please send your abstract
to:
Dr. Lance Urven
University of Wisconsin- Whitewater
800 West Main
Whitewater, WI 53190
urvenl-at-uwwvax.uww.edu
PHONE 414-472-5132
Reception provided by Oxford Instruments Inc.,
Microanalysis Group.:





From: Michael Rock :      merock-at-u.washington.edu
Date: Mon, 13 Mar 1995 10:31:18 -0800 (PST)
Subject: Re: SEM/AFM - CD-recordable, specimen prep.

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Don-
1) the thin film coatings on the polycarbonate are very tightly held
proprietary secrets. try some EDS or WDS.
2) try SEM to determine where these "pits" reside.
3) the "trick" is to plunge the CD into liquid nitrogen (30 sec), then
give it a shot with a hammer. it shears at the interface

we are investigating the CD's by AFM and SEM here in our lab, keep in
touch about further results?

-Mike

On Sun, 12 Mar 1995, Chernoff wrote:

} I am seeking advice regarding specimen preparation. I wish to
} examine the physical pits created when a CD-Recordable disk is
} written. I would like to understand:
} - the physical arrangement of thin film coatings on the
} polycarbonate substrate, i.e. what materials are stacked up, and
} what their typical thicknesses are.
} - what layer contains the physical pits.
} - how to expose that layer for examination by SEM or AFM.
}
} Thanks for your help.
}
} DON CHERNOFF 317-251-1364
} ADVANCED SURFACE MICROSCOPY FAX: 317-254-8690
} 6009 KNYGHTON RD. E-MAIL: echernof-at-ucs.indiana.edu
} INDIANAPOLIS IN 46220 Toll free: 800-374-8557
}
}
}




From: Larry Hawkey :      hawkey-at-neuro.duke.edu
Date: Mon, 13 Mar 1995 14:53:43 -0500
Subject: fair market value for scope?

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Where could I findout what was the fair market value for
a JEOL 1200ex II 5 years old. Basic TEM no Scanning
EDX or stuff like that.
Who has the blue book??

Thank you
larry hawkey
hawkey-at-neuro.duke.edu




From: Davisbl-at-aol.com
Date: Mon, 13 Mar 1995 15:14:53 -0500
Subject: IMAGES

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I'VE BEEN ASKED TO ADDRESS A GROUP OF STUDENTS (LATE HIGH SCHOOL AND EARLY
COLLEGE) A LOCAL COLLEGE SCIENCE CAREER DAY. I AM PLANNING ON USING A
VARIETY OF SEM MICROGRAPHS TO ILLUSTRATE THE APPLICATIONS OF THE SCANNING
ELECTRON MICROSCOPE TO MANY SCIENTIFIC AREAS. IF ANYONE HAS ANY PC-FORMAT
IMAGES THAT THE WOULD BE WILLLING TO E-MAIL TO ME, I WOULD BE VERY GRATEFUL.

THANK YOU ALL IN ADVANCE,
BONNIE DAVIS
E-MAIL:




From: Charles A. Garber
Date: Tue, 14 Mar 1995 02:19:13 EST
Subject: Larry Hawkey; Market Value JEOL 1200EX

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To: Larry Haweky

SPI Supplies

RE: Market Value of JEOL 1200EX

There really isn't anything equivalent to a "blue" book, since there are
just not enough instruments of this type getting sold in order to get any
kind of meaningful sales statistics. However, there are basically three
different types of prices discussed:

a) Selling price of a used instrument from the OEM

b) Selling price from a third party service company

c) Offering price from a former owner


If you want to find out (a), the best person would be Robert Santorelli at
JEOL in Peabody, MA Ph; (800) 343-6766.

For (b), you should contact Mr. Clark Houghton, Secondary Images,
Winchester, OH FAX: (513) 927-5557.

For (c), you should contact again, either Mr. Houghton (above) or Mr. James
Nicolino, X-ray Optics, Florida, Ph: (904) 646-3069 FAX: (904) 565-1897.
Mr. Nicolino services wave length dispersive microprobe systems but has a
good understanding of what different instruments fetch when sold by an
owner, free of any guarantee or warranty, and such sales are generally on
an "as is, where is" basis.

Hope this information will be useful to you.

Charles A. Garber
SPI Supplies
West Chester, PA 19380 USA

Ph: (800) 2424-SPI
FAX: (610) 436-5755
e-mail: GVKM07A-at-prodigy.com





From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Tue, 14 Mar 1995 02:19:01 EST
Subject: RE: ELECTRON OPTICS TEACHING

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--[ FORWARDED PRIVATE MESSAGE ]--------

To: goodhew-at-liverpool.ac.uk Order #4457898
From: GVKM07A
Date: 03/13/95 11:46 PM

Prof. Peter Goodhew

Hello! I think that the soft ware you are thinking of is called the
emTutorial system, and it is indeed produced in Australia. There are
actually two different system programs, emTUTOR and semTUTOR, and they have
been designed to be used as a self-instruction tool, an instruction aid for
class labs and for lecture demonstrations.

Also I think the commercial producers of these really outstanding software
products would be quite unhappy if it should turn out that their
proprietary software was distributed without any benefit to them. I could
be wrong about that, but if I was a betting person, that would be my
opinion.

The product can be ordered from SPI Supplies as SPI #09000-AB and has a
regular price of $345. By coincidence, we have been running a "special"
and until April 15, the price is $250.

There is another new product from the same Australian firm called PARFOCAL,
which is a graphics file conversion program for confocal microscopy and
image analysis. The regular price is $325 but this product is not current
on any kind of special sale.

If you provide a FAX number, we can FAX you additional information.

Charles A. Garber
SPI SUPPLIES (USA)
PO Box 656
West Chester, PA 19380 USA

Ph: (800) 242-4774 Toll free in USA only
(610) 436-5400 Regular phone number
(800) 526-6562 Toll free in Canada only - rings in USA

FAX: (610) 436-5755
1-800-55-7780 Toll free FAX from Ireland only
0800-44-0873 Toll free FAX from New Zealand
0800-89-3066 Toll free FAX from UK



FAX: (610) 436-5755





From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Mon, 13 Mar 1995 22:53:38 EST
Subject: 1st Int'l Conf. EM/Ismalia EGYPT

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I have received the following information from the organizers and wanted to
publicize it to anyone interested in attending or participating:

First International Conference on Electron Microscopy
and
Advances in REsearch in Different Fields of Science

September 2-4, 1995
Ismailia-ETAPT

Sponsored by
Electron Microscopy Center
Suez Canal University
Ismailia EGYPT

Special Topics of the Conference:
1] Role of EM in diagnostic virology
2] Role of EM in diagnosis of tumors cytology and urinary stones
3] Role of EM in ultrastructure pathology of the lung (non neoplastic conditions)
4] X-ray microanalysis: Applications particularly metallurgical,
mineralogical, and biological
5] Scanning EM of plants, animal, insects, and mineral material
6] Study of biological macromolecules from their characteristic electron
diffraction patterns
7] Skin pathology by EM
8] Morphological identification of antigens by EM
9] Different low temperature methods for biological EM
10] Safety measures and maintenance needed for EM

There will be an equipment exhibition in conjunction with this meeting.

Registration for foreigners will be US $150 inclusive of full board during
the time of the meeting.

For further information, contact the organizer:

Prof. Dr. Khalifa Ibrahim Khalifa
Electron Microscope Center
Suez Canal University
Ismailia EGYPT

Ph: (20) 64-226418
(20) 64-333318
FAX: (20) 64-333318 [phone and FAX number]


Message from:

Charles A. Garber
SPI Supplies
PO Box 656
West Chester, PA 19380 USA

Ph: (610) 436-5400
FAX: (610) 436-5755





From: Marcel.Paques-at-2488PAS.urlnl.sprint.com
Date: Tue, 14 Mar 1995 03:41:00 -0500
Subject: mail test m

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X400-Received: by mta merit in /PRMD=internet/ADMD=telemail/C=us/; Relayed; Tue, 14 Mar 1995 03:40:10 -0500
X400-Received: by /ADMD=TELEMAIL/C=US/; Relayed; Tue, 14 Mar 1995 03:38:06 -0500
X400-Received: by /PRMD=SMXFL2/ADMD=TELEMAIL/C=US/; Relayed; Tue, 14 Mar 1995 03:41:49 -0500
X400-Received: by /PRMD=LANGATE/ADMD=TELEMAIL/C=GB/; Relayed; Tue, 14 Mar 1995 03:41:00 -0500

test m




From: Peter Goodhew :      goodhew-at-liverpool.ac.uk
Date: Mon, 13 Mar 1995 08:51:38 GMT
Subject: Re: Electron optics teaching

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--[ FORWARDED PRIVATE MESSAGE ]--------

To: GVKM07A
From: Peter Goodhew
Date: 03/13/95 06:01 AM

David Cockayne asked:
}
} WITH REGARD TO THE QUESTION ABOUT TEACHING OPTICS, CAN ANYONE
ADVISE ME OF WHAT SOFTWARE PACKAGES ARE AVAILABLE FOR TEACHING
ELECTRON OPTICS?

At the same time a similar question was posted on the Microscopy
list (by someone different).

I would be delighted if anyone can update the following list of
teaching software (in the area of microscopy) known to me:

The Institute of Materials (UK) distribute:

The Transmission Electron Microscope by Goodhew
The Scanning Electron Microscope by Humphreys (John)
Electron Diffraction by Goodhew
The Stereographic Projection by Humphreys
Analysis in the Electron Microscope by Goodhew, Humphreys and
Cliff
X-ray Photoelectron Spectroscopy by Watts
X-ray Auger and Laser emission by Goodhew

These are all IBM PC DOS versions, available from Institute of
Materials, 1 Carlton House Terrace, London, SW1Y 5DB, fax
+44(0)171 839 2078
The MATTER project is currently working on greatly-improved
Windows and Mac versions of most of these topics: Completion of
the first two topics (TEM and SEM) is scheduled for the end of
1995. All the above are aimed firmly at teaching, not at research
applications.

There is also an SEM simulation available which originated in
Australia but which is supposed to be downloadable from Nestor
Zaluzec's Microscopy Bulletin Board. However we have consistently
failed to transfer it. Has anyone in the UK succeeded?

I also saw recently a very full simulation of a light microscope.
This is aimed at biologists and was written (and is distributed)
by Maurice Smith at: mol-at-molcol.demon.co.uk

If you can add to this list please reply to both lists [
microscopy and materials-l ]

Thanks

Peter

PS 120+ members of the materials list now!

------------------------------------------------------------ ------
-----
Professor Peter J Goodhew, Department of Materials Science &
Engineering
University of Liverpool
LIVERPOOL Fax (44) (0)151 794 4675
L69 3BX, UK Tel (44) (0)151 794 4665 (secretary
Debra)
------------------------------------------------------------ ------
-----
inter alia: Director of the MATTER project for educational
software
------------------------------------------------------------ ------
-----


-------- Original message header follows --------
From goodhew-at-liverpool.ac.uk Mon Mar 13 06:01:17 1995 [PIM 3.2-342.56]
Received: from AAEM.AMC.ANL.GOV (aaem.amc.anl.gov [146.139.72.3]) by
inetgate.prodigy.com (8.6.10/8.6.9) with SMTP id GAA64180 for
{GVKM07A-at-mail.prodigy.com} ; Mon, 13 Mar 1995 06:01:17 -0500

-------------- End of message ---------------





From: DRStadden:R_D:Armstrong
Date: 3-14-95 8:09am
Subject: AFM Learning Curve

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To: MICROSCO:CENTRAL
cc: DRStadden:R_D:Armstrong
Subj: AFM Learning Curve
------------------------------------------------------------------

One of our researchers is looking into buying an AFM, primarily for
studying latex coatings. As a microscopist (PLM,SEM,EDX) in the
analytical group, I'm interested in the broader applications we might
find for our entire product line.

These are my questions:

1.) What is the "typical" learning curve for the AFM for non-
microscopists vs. microscopists?

2.) How useful is it for one to have access to other types of
microscopies to correlate with AFM results?

3.) Does the usual rule of "fewer operators, less downtime" apply
to any greater or lesser degree with the AFM?

I look forward to any thoughts and experiences you can share.

Dave Stadden
DRStadde+aCENTRAL%ARMSTRONG-at-MCIMAIL.COM
Phone 717-396-5109
FAX 717-396-5865






From: KAKER-at-ctklj.ctk.si
Date: Tue, 14 Mar 1995 14:46:00 +0100 (WET)
Subject: Re:Electron Optics Teaching

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Price list for emTUTOR and semTUTOR (informations from 4th March 1995
direct from Guy Cox Software, Australia):

1. emTUTOR or semTUTOR alone: 100 $
2. Combined package: 160 $
3. Parafocal: 150 S

For detail write or call:

Guy Cox Software
Attn:Guy Cox, M.A., D.Phil.
P.O.Box 366
Rozelle, N.S.W.2039 Australia
Phone & Fax:(02) 818 1896
E-mail:guy-at-emu.su.oz.au

Henrik Kaker
kaker-at-ctklj.ctk.si




From: bjg-at-uniwa.uwa.edu.au (Brendon J. Griffin)
Date: Wed, 15 Mar 1995 00:00:44 +0800
Subject: Australian 'Virtual SEM' teaching package via FTP

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Message-Id: {199503141555.XAA29381-at-uniwa.uwa.edu.au}

Dear All,

I am the author of "Virtual SEM", an earlier version of which called
"SUPERSEM" is available through FTP from the EMMPDL library.

"Virtual SEM 1.2" is an update of this earlier package and is much
enhanced. I'm a bit short of time but the recent discussion (Prof.
Goodhew, and Chuck Garber - regards to both) I think is in part mistaking
my software for that available commercially through SPI and others.

The latest version will be passed to Nestor and John Mansfield in about a
fortnight at the Scanning meeting and I hope they will see fit to update
the old version. It is public domain and comments are most appreciated.
To summarise it is a MAC product utilising real images controlled through a
"SEM console" simulation. It includes tutorial information and two self
assessing tests, the results from which are automatically stored on file.
We have been using it for 18 months and feedback has been good.

The drawback is that it is now around 35 meg in size and I expect it to
reach 40 meg soon. It only needs 4 meg of ram to run on, for example, a
MAC IIsi or better. I developed most of it on a powerbook 180C.

The size is a problem for FTP and as noted in earlier versions I can supply
it on a multisession CD. To do this I am charging US$75 to cover writing
and postal costs only. I will upgrade to later versions and hope to be
able to at postal cost, if I have to hire a bod to do it then would have to
cover the time but cost would still be minimal. We also have a virtual EDS
in development and plan to fully integrate the two.

I will try and answer any queries. Those that have contacted me earlier, I
have not lost your requests, am working my way thru as fast as I can -
please be patient.


Brendon
Brendon J. Griffin
Centre for Microscopy and Microanalysis
The University of Western Australia
Nedlands, WA, AUSTRALIA 6907
ph 61-9-380-2739 fax 61-9-380-1087





From: mullerw-at-rmslab.rockefeller.edu
Date: Tue, 14 Mar 1995 10:59:43 EST
Subject: Microscopy listserver

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Dear Sir or Madam:

I am an Associate Professor at The Rockefeller University interested
in obtaining access to the microscopy listserver. A colleague told me that
this would be a good medium to advertise postdoctoral and technical positions
that may be available in my laboratory.

Please let me know if I need to submit any more information. My address
is:
William A. Muller, MD, PhD
Box 176
The Rockefeller University
1230 York Avenue
New York, NY 10021
Phone (212) 327-8104
Fax (212) 327-8875

e-mail address: mullerw-at-rmslab.rockefeller.edu

Thank you for your help.

Sincerely,


Bill Muller






From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 14 Mar 1995 16:31:43 -0400
Subject: Int'l MAS Conf??

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Message-ID: {n1416923531.369-at-mse.engin.umich.edu}

Subject: Time: 4:23 PM
OFFICE MEMO Int'l MAS Conf?? Date: 3/14/95

I have heard rumors that there will be an international microbeam analysis
meeting in Australia early in 1996. Does anyone know if
this is so; and if it is, do you have any information about when and
where it will be?





From: almonte-at-medcolpa.edu
Date: Tue, 14 Mar 1995 17:04:50 -0400
Subject: FM crossover between FITC & Rhodamine

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I'm staining spinal cord and medulla's neurons from rats' fetuses, 10 days
old, with the following primaries antibodies: Rabbit anti Glycine 1:2500,
1:4000; Rabbit anti GABA 1:100, 1:200; Rabbit anti GAD 1:300, 1:500; and
anti-synaptophysin 1:150, 1:300. I'm using FITC 1:100 or Rhodamine 1:100 as
my secondary antibodies. I keep getting crossover between FITC and
Rhodamine despite the different dilutions of the primary antibodies I have
used. Does anyone has any suggestion?
Thanks in advance,

Ciprian Almonte
Research assistant
Medical College of Pennsylvania
Dept. of Anatomy and Neurobiology
3200 Henry Ave.
Philadelphia, PA 19129
Voice: (215) 842-4081







From: Dave DeFily :      defily-at-tam2000.tamu.edu
Date: Tue, 14 Mar 1995 16:28:18 -0600
Subject: 1.53 vs. 1.57

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We've been trying to measure "integrated density" of some fluorescent images and
found significant differences in the numbers calculated in v. 1.53 and 1.57. We
have 1.53 on a quadra 950 and 1.57 on an 8100. Working with the same image,
1.53 returns numbers approximately 2,000,000 and 1.57 gives us numbers about
400. The mean intensity, Std dev, background, number of pixels...are identical
in both versions. LUTs are also the same in both versions. These are TIFF
images acquired on a DOS machine. I'm not sure if it's related to the
integrated density difference, but in 1.57 we can "open" the TIFF files, while
in 1.53 we have to "import" the TIFF file.

Is there something obvious I'm overlooking? Any suggestions are appreciated.
Thanks

-Dave defily-at-tamu.edu







From: almonte-at-medcolpa.edu
Date: Tue, 14 Mar 1995 17:40:19 -0400
Subject: FITC and Rhodamine crossover????

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I'm staining spinal cord and medulla's neurons from rats' fetuses, 10 days
old, with the following primaries antibodies: Rabbit anti Glycine 1:2500,
1:4000; Rabbit anti GABA 1:100, 1:200; Rabbit anti GAD 1:300, 1:500; and
anti-synaptophysin 1:150, 1:300. I'm using FITC 1:100 or Rhodamine 1:100 as
my secondary antibodies. I keep getting crossover between FITC and
Rhodamine despite the different dilutions of the primary antibodies I have
used. Does anyone has any suggestion?
Thanks in advance,

Ciprian Almonte
Research assistant
Medical College of Pennsylvania
Dept. of Anatomy and Neurobiology
3200 Henry Ave.
Philadelphia, PA 19129
Voice: (215) 842-4081







From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 14 Mar 1995 22:45:49 -0600 (CST)
Subject: FTP & WWW at ANL site

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G'day Subscribers....

A general posting since I've had a bunch of questions all
about the same thing.

Yes the EMMPDL/MASLIB/PD Shareware libraries are avaiable
via anonymous FTP. The site is

WWW.AMC.ANL.GOV (= 146.139.72.10 )

Login via standard FTP protocols with
Username=Anonymous
Password=Your Email Address

but please note that you CANNOT access these files using a WWW Browsing program
like Mosiac, Netscape or any other, even though they have FTP
capabilities. You must use a standard FTP protocol client program.
which accesses the conventional FTP ports.

The reason for this is that the although WWW server and the FTP
server are both on the same Computer (WWW.AMC.ANL.GOV=146.139.72.10)
they reside on two different disk partitions which are NOT linked.
The FTP server looks in one place and the WWW server another.
When you login to the WWW site, your browser (client) program
has only access to the disk space assigned to the WWW server hence
it (the WWW program) will NEVER see the FTP files and vice versa!
The reasons for this are a combination of security and convenience
on my part, but with the explosion of the WWW I've created yet
another headache for myself.

So for all of you that have tried and failed this is the most
common reason. If you don't understand this call me next week
sometime and I'll explain, or better yet come to the Telecommunications
Tutorial which I'll be giving at the August MSA meeting.

In retrospect I should have put them on different machines with
different DN's and then the problem would not have happened, but
the damage is done and until I get a chance to come up for air
it will have to stay that way. There is yet another alternative
which is to define an alias on the DNS for the FTP server. If
I can figure out the right person to contact I'll try that route
but be guarenteed it will not be soon.

Just a reminder.....
Abstracts/Papers for the August MSA meeting are due March 15th
i.e. probably the day you receive/read this message.

Cheers...
Your Friendly Neighborhood SysOp

Nestor








From: Greg Begin :      GREGB-at-sales.lmt.com
Date: Wed, 15 Mar 1995 08:11:43 CST6CDT
Subject: ? August Microscopy Meeting

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Howdy All,

If anyone has information on the Microscopy Meeting in August
could you please send information on contacts for the meeting.

Also, I am looking to attend regional meetings and would love to
find a calendar of meetings and places with contacts. I would be
attending for training and exibiting.

Thank You all!!!

P.S. all that are interested about the LaserMaster ImagePrinter
1800 DPI the "NEW" price is $ 6,995.00 and an upgrade from a 60
mhz processor to a 100 mhz. WOW, 1K off plus upgrade.

Greg Begin - LaserMaster Imaging
Specialist in Scientific/Medical Imaging




From: :      MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Wed, 15 Mar 1995 07:23:22 +0800PST
Subject: FITC and Rhodamine crossover????????

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Just read the posting by Ciprian Almonte concerning crossover between
FITC and Rhodamine and had a couple of questions about what he is
doing. What are your FITC and Rhodamine linked too--goat
anti-rabbit??? Are you trying to do double labelling??? Or is it
that you are getting fluorescence of the FITC at the rhodamine
settings?? More information is required to help.
Yours
Mark Elliott, PhD
UBC-Pulmonary Research Laboratory,
St. Paul's Hospital
Vancouver BC Canada




From: gkennedy-at-ucsd.edu
Date: Wed, 15 Mar 1995 07:42:33 -0800
Subject: FITC/rhodamine

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You need to "fine tune" your microscope filters. Do you perhaps have an older Olympus?? Call your local microscope rep and ask for the correct filters to narrow the band pass of the dichroic filters. Grace






From: :      MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Wed, 15 Mar 1995 07:19:05 +0800PST
Subject: epon alternatives

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I think this may have been discussed by this group earlier but at the
time we were not worried about it so I didn't keep a record of the
responses. One of our tech's asked me to post this:
We are looking for a plastic medium which can replace Epon in
Biological TEM. In our lab we regularly use Epon 812 for its good
stainability of membranes. Recently however, we have just too many
samples to infiltrate and Epon is just too viscous for multiple
tissue transfers. The result is extensive numbers of labour hours.
Please let us know if you have succcessfully worked with any plastic
that has equal stainability of the membranes but is less viscous.
Thanks
Mark Elliott
UBC-Pulmonary Research Laboratory
St. Paul's Hospital
Vancouver BC Canada




From: almonte-at-medcolpa.edu
Date: Wed, 15 Mar 1995 13:42:20 -0400
Subject: Re: FITC and Rhodamine crossover????????

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} Just read the posting by Ciprian Almonte concerning crossover between
} FITC and Rhodamine and had a couple of questions about what he is
} doing. What are your FITC and Rhodamine linked too--goat
} anti-rabbit??? Are you trying to do double labelling??? Or is it
} that you are getting fluorescence of the FITC at the rhodamine
} settings?? More information is required to help.
} Yours
} Mark Elliott, PhD
} UBC-Pulmonary Research Laboratory,
} St. Paul's Hospital
} Vancouver BC Canada

FITC and Rhodamine are linked to anti-rabbit, and I'm planning to do double
labelling. My major problem is that I'm getting flourescence of the FITC at
the rhodamine settings and viceversa. Thanks, to all of those that have
replied so far. I appreciate all their suggestions.
Ciprian

Ciprian Almonte
Research assistant
Medical College of Pennsylvania
Dept. of Anatomy and Neurobiology
3200 Henry Ave.
Philadelphia, PA 19129
Voice: (215) 842-4081
E-mail almonte-at-medcolpa.edu







From: Wayne A. Buttermore :      butterw-at-iia.org
Date: Wed, 15 Mar 1995 18:00:25 -0500 (EST)
Subject: Unsubscribe

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Unsubscribe butterw-at-iia.org





From: MacisNo1-at-aol.com
Date: Wed, 15 Mar 1995 21:12:05 -0500
Subject: FITC/ Rhod

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Try using more selective excitation filters (narrower band pass). Another
suggestion is to use a "Red Cut" Filter. This greatly supresses the secondary
peak that is associated with FITC. Contact you local microscope supplier or
Omega Optical at (802) 254-2690. Good Luck,

Lawrence Kordon
Nikon, Inc.
(410) 740-7404




From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Thu, 16 Mar 1995 15:50:13 +1100
Subject: SEM of Microcapsules

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Hello all,
We have a user wanting to look at the inside of Polycaprolactone
Microcapsules in the SEM. We are not wanting to do sections for the TEM.
I have tried to fracture the microcapsules in liquid N2, after embedding
them in resin, but they dont fracture through the capsule, only around it.
Also, I have tried to section the block face of the capsules in resin, but
resin seems to infiltrate into the capsule and so no internal structures
can be seen.
These capsules are between 100 microns - 20 microns in diameter, and have a
melting point around 65oC.
Has anyone tried this before and had success? The references I have
contain no details about preparation of their specimens.
Any help would be appreciated.

Richard Lander.

richard.lander-at-stonebow.otago.ac.nz






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Wed, 15 Mar 1995 22:54:12 -0600 (CST)
Subject: Int. MAS Meeting (IUMAS)

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G'day Subscribers (actually in it's now about 11 pm in Chicago)

Here's the info that was posted about the IUMAS meeting that
I dug out of the Archives. You will have to check with Dave
Williams of Lehigh (dbw1-at-lehigh.edu) if you want further details.

Also the WWW site is also starting to get fairly busy, lately
we've been averaging ~ 250 connections/day. So if it
appears slow, consider yourself forwarned.

The FTP server crashed sometime on Monday Evening.
So if anyone tried to get to the libraries, it was
a useless battle. As of a few minutes ago it was back
up and running.


Why does this always happen just before deadlines for
MSA abstracts?

It must be the Ides of March syndrome,
but then again maybe it was a Leprechaun.

G'night all.... Nestor

-------------------------------------------------------------



From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Wed, 15 Mar 1995 22:54:12 -0600 (CST)
Subject: Int. MAS Meeting (IUMAS)

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MICROBEAM ANALYSIS SOCIETY STUDENT SCHOLARSHIPS TO MAS-1995 (Breckenridge, CO)
and IUMAS-1 (Sydney Australia)

All students (and faculty) involved in microanalysis-related research, should
note a remarkable opportunity for travel to two forthcoming microanalysis
conferences. The Microbeam Analysis society is offering student scholarships to
the 29th Annual MAS meeting in Breckenridge CO (August 6-11, 1995). Student
papers must be submitted, in standard MAS-MSA format to Dr. E. Etz, NIST,
Division 837, Bldg. 222, room Q-113, Gaithersburg, MD 20899 by March 15, 1995.
The best submitted papers will be awarded funds towards attending the annual
meeting in Breckenridge. Any more information about the program can be obtained
from Dr. Etz at etz-at-gapnet.nist.gov

What makes this scholarship offer extraordinary is that the best three papers
given by student scholarship winners at the Breckenridge meeting will be
awarded a minimum of $500 towards the cost of attending the 1st
International Union of
Microbeam Analysis Societies Conference in Sydney, Australia, February 5-9,
1996. These scholarships are only open to student members of MAS, and student
application forms for MAS are available in past issues of Microbeam Analysis,
the MAS journal. Student membership is a great bargain at $2.50, and doesn't
require that the advisor be a MAS member - although if you aren't, I ask that
you consider joining.

I will mail you more information with appropriate details about the meetings
and the paper format etc., but if you have any immediate questions, please don't
hesitate to contact me by email (dbw1-at-lehigh.edu).

Dave Williams






From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Thu, 16 Mar 1995 08:01:30 -500
Subject: Epoxy resin dust saftey

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Can anyone out there point me in the right direction for finding
information on the health risks associated with epoxy resin dust
produced by jewler's saws, jig saws, dremel tools, files, sand paper,
etc.. Yes, I have looked at the various MSDS for the resins but they
haven't provided much real world information (I at least haven't run
into the problem of a tanker truck spillage of NSA).


Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Biological Science Building
Miami University, Oxford, OH 45056
Ph: 513-529-5712
E-mail: edelmare-at-muohio.edu




From: mrdunlap-at-ucdavis.edu (Michael Dunlap)
Date: Thu, 16 Mar 1995 08:11:26 -0800
Subject: Re: SEM of Microcapsules

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Richard -

We use double stick tape to fracture the Microcapsules. On a SEM stub we
mount an stip of tape. On tape that is covering the stub we add a small
amount of dried microcapsules. Using an exta piece of tape cover the tape
and capsules and rip off. This will fracture many of the microcapsule so
that you will be able to look inside and outside many of them. Coat the
stubs and view in the SEM.

Good luck

Mike

---------------------------------------------------------------------------
| Michael Dunlap | lab (916) 752-0284 |
| Facility For Advanced Instrumentation | fax (510) 422-2282 |
| University of California | mrdunlap-at-ucdavis.edu |
| Davis CA, 95616 | |
===========================================================================






From: Louisa.Howard-at-Dartmouth.EDU (Louisa Howard)
Date: 16 Mar 95 11:16:20 EST
Subject: TEM and LM on cell monolayer

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Message-id: {14465545-at-blitzen.Dartmouth.EDU}

I have done many TEM preparations on cell monolayers, using tissue culture
dishes and/or glass coverslips. There is a lab here that would like to do light
microscopy and TEM on a single cell in a monolayer. I assume this can be done,
if I had a coverslip that was etched with a marker grid. This marked grid
pattern would have to show on the epoxy face, after the coverslip was separated
from the embedding media. Can anyone tell me if there are glass or plastic
coverslips that have some type of etched grid pattern that could be used in
this way and where they could be ordered in the U.S.

I would prefer working with a plastic/tissue culture type of coverslip, as
they are easier to remove before sectioning.

thanks
Louisa Howard
Ripple Electron Micrscope Facility
Dartmouth College
Hanover, N.H 03755
Louisa.Howard-at-Dartmouth.edu




From: Louisa.Howard-at-Dartmouth.EDU (Louisa Howard)
Date: 16 Mar 95 11:36:20 EST
Subject: TEM and LM of cell monolayer

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Message-id: {14465851-at-blitzen.Dartmouth.EDU}

I have done many TEM preparations on cell monolayers, using tissue culture
dishes and/or glass coverslips. There is a lab here that would like to do light
microscopy and TEM on a single cell in a monolayer. I assume this can be done,
if I had a coverslip that was etched with a marker grid. This marked grid
pattern would have to show on the epoxy face, after the coverslip was separated
from the embedding media. Can anyone tell me if there are glass or plastic
coverslips that have some type of etched grid pattern that could be used in
this way and where they could be ordered in the U.S.

I would prefer working with a plastic/tissue culture type of coverslip, as
they are easier to remove before sectioning.

thanks
Louisa Howard
Ripple Electron Micrscope Facility
Dartmouth College
Hanover, N.H 03755
Louisa.Howard-at-Dartmouth.edu




From: Louisa.Howard-at-Dartmouth.EDU (Louisa Howard)
Date: 16 Mar 95 12:56:52 EST
Subject: TEM and LM of cell monolayer

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Message-id: {14466868-at-blitzen.Dartmouth.EDU}

I have done many TEM preparations on cell monolayers, using tissue culture
dishes and/or glass coverslips. There is a lab here that would like to do light
microscopy and TEM on a single cell in a monolayer. I assume this can be done,
if I had a coverslip that was etched with a marker grid. This marked grid
pattern would have to show on the epoxy face, after the coverslip was separated
from the embedding media. Can anyone tell me if there are glass or plastic
coverslips that have some type of etched grid pattern that could be used in
this way and where they could be ordered in the U.S.

I would prefer working with a plastic/tissue culture type of coverslip, as
they are easier to remove before sectioning.

Thanks.

Louisa Howard
Ripple Electron Micrscope Facility
Dartmouth College
Hanover, N.H 03755
Louisa.Howard-at-Dartmouth.edu




From: nina allen :      allen-at-wfu.edu
Date: Thu, 16 Mar 1995 14:54:55 -0500 (EST)
Subject: Re: TEM and LM of cell monolayer

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If you are going to look at cells in a light microscope, it is a poor
idea to use plastic coverslips. You will not get a good image in
Differential Interference Contrast or Polarized light...as the cover slip
may be strained, and so forth. N.Allen




From: sfzane-at-unccvm.uncc.edu (Sandra F. Zane)
Date: Thu, 16 Mar 1995 15:32:58 -0500
Subject: curing resins

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Hello Microscopists,
Although I can't help Richard Edelmann with his question regarding
resin dust except to say that we do use dust masks when sawing or cutting
blocks; I would like to pose a question closely related to this.
How many of you have your curing ovens vented to a hood?
We have a VERY low volumn of resins being cured at any given time and
have sought, somewhat unsuccessfully, information that would help us
determine at what level it would be necessary to do this. As Richard said,
the MSDS sheets don't provide much real world information. I did call one
of the EM suppliers (I don't recall which one now), and was told that
venting to a hood would be necessary only in an industrial situation.
I would be very interested in hearing your opinions and how you handle
this problem.
I would also be willing to summarize any responses and post that to the
list.

Thank you very much, Sandra Zane

Sandra F. Zane, EM Tech.
Biol. Dept. UNCC
Charlotte, NC 28223
sfzane-at-unccvm.uncc.edu
Fax (704) 547-3128





From: Patty Jansma :      plj-at-manduca.neurobio.arizona.edu
Date: Thu, 16 Mar 1995 13:52:43 -0700 (MST)
Subject: Re: TEM and LM of cell monolayer

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We use glass gridded coverslips for tracking cells. You can get them from
Bellco Glass, Inc. P.O. Box 340 Edrudo Road Vineland, New Jersey 08360
Cat. # 1916 -92525 Etched gridded coverslips 1 case approx. $30.00.


Patty Jansma Tel:602-621-6671
plj-at-manduca.neurobio.arizona.edu
Arizona Research Labs Division of Neurobiology
University of Arizona


On 16 Mar 1995, Louisa Howard wrote:

} I have done many TEM preparations on cell monolayers, using tissue culture
} dishes and/or glass coverslips. There is a lab here that would like to do light
} microscopy and TEM on a single cell in a monolayer. I assume this can be done,
} if I had a coverslip that was etched with a marker grid. This marked grid
} pattern would have to show on the epoxy face, after the coverslip was separated
} from the embedding media. Can anyone tell me if there are glass or plastic
} coverslips that have some type of etched grid pattern that could be used in
} this way and where they could be ordered in the U.S.
}
} I would prefer working with a plastic/tissue culture type of coverslip, as
} they are easier to remove before sectioning.
}
} Thanks.
}
} Louisa Howard
} Ripple Electron Micrscope Facility
} Dartmouth College
} Hanover, N.H 03755
} Louisa.Howard-at-Dartmouth.edu







From: sfzane-at-unccvm.uncc.edu (Sandra F. Zane)
Date: Thu, 16 Mar 1995 16:08:03 -0500
Subject: EM:curing resins

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Hello Microscopists,
Although I can't help Richard Edelmann with his question regarding
resin dust except to say that we do use dust masks when sawing or cutting
blocks; I would like to pose a question closely related to this.
How many of you have your curing ovens vented to a hood?
We have a VERY low volumn of resins being cured at any given time and
have sought, somewhat unsuccessfully, information that would help us
determine at what level it would be necessary to do this. As Richard said,
the MSDS sheets don't provide much real world information. I did call one
of the EM suppliers (I don't recall which one now), and was told that
venting to a hood would be necessary only in an industrial situation.
I would be very interested in hearing your opinions and how you handle
this problem.
I would also be willing to summarize any responses and post that to the
list.

Thank you very much, Sandra Zane

Sandra F. Zane, EM Tech.
Biol. Dept. UNCC
Charlotte, NC 28223
sfzane-at-unccvm.uncc.edu
Fax (704) 547-3128





From: jthompso-at-wiley.csusb.edu (Jeff Thompson)
Date: Thu, 16 Mar 1995 14:00:42 -0800
Subject: Re: TEM and LM of cell monolayer

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Glass coverslips etched with an alphanumeric grid are available
from Bellco Glass, Inc. (800-257-7043). I have successfully grown cells in
culture on these coverslips for LM and SEM analysis after coating the
coverslips with either poly-lysine or collagen. Identified cells are
relatively easy to re-identify when going from one system to the other. I
have not embedded cells grown on these coverslips, so I do not know if the
grid will show up on the surface of the plastic when the coverslip is
removed.

Jeff Thompson, Ph.D.
Director, Electron Microscope and Image Analysis Center
California State University
San Bernardino, CA 92407
jthompso-at-wiley.csusb.edu





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 16 Mar 1995 23:50:47 -0600 (CST)
Subject: Nanotubes & X-sections

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Message-Id: {199503170423.AA27446-at-pccms.pku.edu.cn}


I must confess I'm a bit perplexed by Z.Q. Liu's question about
x-sections of Nano-tubes. If they are truely "nanometer" in size
why would you attempt to x-section them? Are they actually larger?
All the EM I've seen about nanotubes did not involve x-section, but
simple direct imaging.

Okay so let me ask the dumb question. How big are they really?

Nestor....
Your Friendly Neighborhood SysOp.




From: Leszek Kepinski :      kepinski-at-highscreen.int.pan.wroc.pl
Date: Fri, 17 Mar 1995 12:09:15 +0100 (CET)
Subject: HRTEM negatives - digitalization

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What is the appropriate way to digitize TEM negatives for HRTEM work at
resolution of approx.0.23 nm? Please give approximate cost of the digitizing
hardware when possible.
Leszek Kepinski


*****************************************************************
Leszek Kepinski
Institute of Low Temperature and Structure Research,
Polish Academy of Sciences, P.O.Box 937, 50-950 Wroclaw, Poland
tel:(4871) 350 21 ext.153
fax:(4871) 44 10 29
email: kepinski-at-highscreen.int.pan.wroc.pl
*****************************************************************




From: Carl Henderson :      chender-at-umich.edu
Date: Fri, 17 Mar 1995 09:48:00 -0500 (EST)
Subject: Re: Mprobe.Geol

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On Fri, 17 Mar 1995, Robert McDonald wrote:

} I have been asked to analyse some micas for Rb and from a
} preliminary examination it looks like I should use the L alpha
} line - except that the Si K alpha is rather close making the
} Rb sit on the slope to the right of it.
}
} Anyone have any advice on backgrounds etc?
}
} I am using TAP xtals, 25 kV,55 nA with a counting time of
} 60 seconds for peak and background - is this reasonable?
}
} Machine is Cameca SX50.

25kV, 55 nA sounds pretty high for routine mica analyses, though it might
be OK to analyse the Rb at this condition after analysing the major
elements. At 25kV, you could use the Rb KA line on an LIF crystal and
avoid the Si overlap. The counting times sound reasonable, though you
may have to increase them if you are really interested in "trace"
analysis of low levels of Rb.

I suspect that you will always obtain "false" Rb when analysing Rb LA on
TAP in the presence of high Si in your sample. The Si KA peak is about
-750 steps from the Rb LA line (and about +200 from the Rb LB line). This
is far enough that most of the Si peak will be resolved, but there will
probably be some tail effect of the Si under the Rb LA peak. You can
check for this be analysing SiO2. This will give you the worst case Si
interference for silicates. You could continue this process and draw up a
working line (curve) of wt.% Si vs. wt.% measured Rb by measuring various
silicates with differing Si contents and no Rb. You could then use this
curve to correct for your analyses of Rb on your unknowns.

The SX50 software should also give you the chance to use a background
"slope" instead of having to take background counts at both +bkgd and
-bkgd positions. The "slope" on the older CAMECA MBX software was defined
as (bkdg+ + bkgd-)/(2*bkgd+). Thus, a "flat" background would have a
slope of 1.000, while a background that increases with lower sin(theta)
will have a slope } 1.000. Using a background slope will not solve the
tail effect of the Si under the Rb LA, but may help you in using the Rb KA
line on LIF, where the bkgd- position may be out of range for the
spectrometer.

Good luck!

Carl

Carl Henderson
Electron Microbeam Analysis Laboratory
University of Michigan
2005 C.C. Little Bldg.
Ann Arbor, MI 48109-1063 USA
-----------------------------------------------------
(313) 936-1550 (voice) **** Next time, ****
(313) 763-4690 (FAX) *** take the ***
chender-at-umich.edu (e-mail) **** train! ****
-----------------------------------------------------




From: tivol-at-tethys.ph.albany.edu
Date: Fri, 17 Mar 1995 10:33:50 EST
Subject: Re: Nanotubes & X-sections

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Dear Nestor,
John Cowley gave me reprints of some of his recent papers in which
there are images of nanotubes which are on the order of 10 nm across (O.D.).
He and his co-workers have found different structures at various places in
the nanotubes, so I can see why someone might wish to section them (although
it is hard to imagine how this can be done without perturbing (destroying?)
the structures). John would likely send you reprints if you asked him.
Yours,
Bill Tivol




From: tivol-at-tethys.ph.albany.edu
Date: Fri, 17 Mar 1995 10:55:30 EST
Subject: Re: HRTEM negatives - digitization

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Dear Leszek,
In order to retain the original resolution (0.23 nm), the digitization
should have a pixel size corresponding to no more than half that resolution.
That is, if the magnification of the negative is 100,000x, the pixel size must
be ~10 micrometers. This can be done on (among other brands) a Perkin-Elmer
flatbed microdensitometer (cost ~ $100,000). A much cheaper alternative is
a CCD camera. By arranging to image the negative with the CCD so that the
pixels are ~10 micrometers you can get the required resolution. This means
that a 1000*1000 CCD array will look at a 1 cm**2 area. This can be done at
a cost of ~$50,000 (maybe less--I'm no expert here). You also save scan time
with the CCD; however, quantitation of intensities--especially for ED patterns
etc. where there are regions of very high OD surrounded be regions of low OD--
is much worse with CCD than with a flatbed scanner. Good luck.
Yours,
Bill Tivol




From: Larry Allard :      allardlfjr-at-ma160.ms.ornl.gov
Date: 17 Mar 1995 08:42:26 U
Subject: nanotube x-sect.

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Message-ID: {n1416690645.11463-at-ma160.ms.ornl.gov}

Subject: Time: 8:16 AM
OFFICE MEMO nanotube x-sect. Date: 3/17/95

Can standard TEM images normal to the axis of the nanotube determine
unambiguously the cross sectional shape of tubes (i.e. whether the tubes are
facetted, circular cylinders, oval-shaped, hollow or filled with amorphous
material, etc?). We think there are 2 ways to get this information: direct
imaging of cross-sections or electron holography (in which case you don't
need cross-sections). The problem with cross-sections is the potential
development of artifacts during the preparation process. We suggest strongly
to use electron holography (see for example: J. Mater. Sci., 29 (1994)
5612-5614 (this is on Pd particles, but the principle is the same). Also see
our paper which includes nanotubes in the Proceedings of the 1st Int'l
Workshop on Electron Holography, Elsevier, in press.





From: ldm-at-apollo.numis.nwu.edu (L. D. Marks)
Date: Fri, 17 Mar 1995 11:56:54 -0600
Subject: Re: HRTEM negatives - digitization

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Bill Tivol said in his email that "the digitization should have a pixel size corresponding to no more than half that resolution". I think that this is a
typo: you need at least 2 sampling points for a given wave. Therefore if
one wants to quantify 0.23nm spacings in HREM the smallest sampling is half
this (Nyquist limit) and } 6 samplings is more standard.




From: Larry Allard :      allardlfjr-at-ma160.ms.ornl.gov
Date: 17 Mar 1995 15:35:47 U
Subject: Digitizing TEM negatives

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I've repeated the original question below, in case someone's missed the
thread. I suspect that Liu is trying to make cross-sections to
characterize growth mechanisms. Nanotubes, like asbestos fibers, are
easily examined in plan-view, since their high aspect ratio virtually
guarantees that they will lie flat on a film with the long axis parallel
to the film surface. Most of the pictures that I have seen claiming to
be nanutubes in cross-section had no supporting evidence that they were
anything but an essentially spherically symmetric "bucky onion."

In order to get a true nanotube cross section, one will first probably
have to anneal the mass to remove all lesser fullerenes, including the
onions. Then break the mass up and embed in a hard epoxy. Although
ultramicrotomy isn't a bad idea, there is no real way to align the
nanotubes and I'm not certain what effect this would have on cutting.
It is probably better just to sandwich between silicon and do a standard
cross-section by mechanical polishing, dimpling, and ion-milling. It is
going to be hard to prevent rapid milling of the epoxy as opposed to the
tubes, so it is important to mechanically thin as far as possible before
ion-milling (hence, the silicon as an optical guide).

That's my 2 cents worth.

Daniel L. Callahan
Department of Mechanical Engg. and Materials Science
Rice University
dlc-at-owlnet.rice.edu
---------- Forwarded message ----------

Subject: Time: 3:27 PM
OFFICE MEMO Digitizing TEM negatives Date: 3/17/95

Regarding the recent debate on negative scanning:
The Ektron scanner is {$30K, and gives a highly linear image up to 4096 x
4096 pixels. It will permit accurate density corrections to be done for best
quantitative results (see Voelkl, et al., Ultramicroscopy 55 (1994) 75-89,
for all you could ever want to know about scanning negatives and density
corrections etc.)





From: tivol-at-tethys.ph.albany.edu
Date: Fri, 17 Mar 1995 16:22:54 EST
Subject: Re: Re: HRTEM negs - digit

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L.D. Marks is correct--I meant a pixel size corresponding to twice the reso-
lution, or half the size. I always seem to have trouble writing about things
for which less is better :-).
Yours,
Bill Tivol




From: MILLERS-at-NCCCOT.AGR.CA
Date: 17 Mar 1995 10:17:34 -0500 (EST)
Subject: microscopical society of canada annual meeting

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Microscopical Society of Canada
22nd Annual Meeting

The MSC Executive and the Local Organising Committee cordially invite you to attend
and participate in the 22nd Annual Meeting of the Microscopical Society of Canada.
This meeting will be held in the University Centre Building, University of Ottawa, Ottawa,
Ontario, Canada, June 4-7, 1995. A varied and interesting scientific program has been
planned and will consist of a combination of interdisciplinary symposia presented by
speakers from around the world, separate physical and biological symposia, oral and
poster presentations, workshops on TEM specimen preparation of materials and cryo-
SEM specimen preparation and X-ray analysis, and commercial exhibits.

Local Organising Committee

Jim Corbett (Chairman, Secretary, University of Ottawa Liaison)
John McCaffrey (Treasurer, Space Management, Social Program, Accommodation)
Jeff Fraser (Commercial Exhibits, Social Program, Accommodation)
Graham Carpenter (Scientific Program Chair, Materials)
Larry Arsenault (Scientific Program Chair, Biology)
Peter Sewell (Corporate Liaison, Commercial Exhibits)
Kamal Botros (Scientific Program)
Louise Weaver (Scientific Program)
Paula Allan-Wojtas (Registration)
Shea Miller (Registration)

DEADLINE FOR RECEIPT OF ABSTRACTS: March 31, 1995

DEADLINE FOR PRE-REGISTRATION: May 1, 1995

For further information contact:

Program: Registration:

Jim Corbett Shea Miller or Paula Allan-Wojtas
Department of Physics Centre for Food and Animal Research
University of Waterloo Agriculture and Agri-Food Canada
Waterloo, Ontario Room 2016, K.W. Neatby Bldg.
Canada, N2L 3G1 Central Experimental Farm
Ottawa, Ontario, Canada, K1A 0C6
Tel: (519) 885-1211 Tel: (613) 957-4347, ext. 7908 (Shea),
7970 (Paula)
Fax: (519) 746-8115 Fax: (613) 943-2353
e-mail: corbett-at-physics.watstar.uwaterloo.ca e-mail: millers-at-ncccot.agr.ca
allanwojtasp-at-ncccot.agr.ca


PRELIMINARY MEETING PROGRAM

INTERDISCIPLINARY SYMPOSIUM
ADVANCES IN MICROSCOPY


J. Watson (Michigan State University) Events in science and microscopy
J. Hillier (Princeton University) Early developments in EM
A. Eades (University of Illinois) Electron microscopes of the future
O. Krivanek (Gatan Inc) Nanoscale elemental and chemical
mapping
M. Hansen (FEI Inc.) A comparison of LaB6 and CeB6
thermionic cathodes
I.A. Rauf (Queen's University) STM, AFM, CTEM and STEM imaging
of polycrystalline tin-doped indium
oxide thin films
H.J. Kreuzer (Dalhousie University) Lensless low-voltage electron
holography

MATERIALS SCIENCES

D. Dorset (University of Buffalo) Direct structure determination by
electron crystallography
A. Eades (University of Illinois) RHEED: progress towards the
extraction of quantitative information
P. Midgley (University of Bristol) Electron diffraction for structure
determination
D. McCombe (McMaster University) Scanning tunnelling microscopy of
semiconductor surfaces and
interfaces
D. Perovic (University of Toronto) Direct imaging of semiconductor
dopants by EM
M. Loretto (University of Birmingham) Analytical TEM of advanced
materials
G. Weatherly (McMaster University) Microstructures of SiC-reinforced Mg
casting alloys
C. Hansen (Queen's University) The application of environmental
SEM to studies of concrete

BIOLOGICAL SCIENCES

W. Chiu (Baylor College of Medicine) Cryo-imaging
P. Ottensmeyer (University of Toronto) Imaging and computer analysis
H. Hagler (University of Texas) Calcium imaging
P. Ingram (Research Triangle Inst) Elemental mapping
P. Echlin (Cambridge University) Cryo-methods


WORKSHOPS



TEM specimen preparation for Materials Science
Topics include: Ion-beam sputtering
Cleaving
Ultramicrotomy
Cross sections, etc.



Cryo-SEM specimen preparation and X-ray analysis
Topics include: Fracturing and cryo-planing for morphometric and elemental
analysis
Cryo-conductive coating
Standards for frozen hydrated specimens
Light element detection and quantification



Satellite Workshop (June 8, Central Experimental Farm, Ottawa)
Applications of Microscopy in Agricultural Research
Topics include: Preparative techniques
3-D view of the cell
Microtubules
Soils in agricultural use
Electron microscopy in dairy research
Entomology
Image analysis-seed quality
Image analysis of SDS-PAGE gels
Spore development
Immunogold labelling
Food microstructure




From: DVCCO-at-aol.com
Date: Sun, 19 Mar 1995 03:54:20 -0500
Subject: Video Camera Selection Guide2of3

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DVC Video Camera Guide And Release 2 of 2

Remember, if the camera is on the ragged edge of 8 bit performance and
you want to add gain for more sensitivity, even the smallest amount of
gain might put you in the 7 bit range if you we not there already.
If you have 10 bit capability on your board why not use a 10 bit camera.
* Also a camera specification of 60dB is not a 10 bit camera only an 8.
* Also there are many types of noise in a sensor besides thermal noise.
Cooling a sensor will reduce (thermal noise only) and allow you to
integrate longer. I would double check the impression some make that
when a 50db sensor is cooled it offers a better s/n of 10 or 12 bits vs
just 7.
The reduction of one type of noise (thermal) is all that cooling
provides.
* Other types of sensor noise besides dark current include photon, nyquest,
shot, gain noise, and flicker noise etc.
* The signal to noise of the sensor at room temperature is a good measure
of what you can expect.
** Look at the quantum efficiency at the nm range needed.
** look at the spectral response of the sensor.
** There are different grades of sensors available relative to dead
pixels and gray level variation on other manufacturers cameras.
*Digital RS-422 eliminates pixel jitter for sub-pixel accuracy if it is
needs versus the genlock action between the digitizer board and the
camera as they try to lock to each other in the RS-170 analog mode.
*Digital cameras of the higher end variety typically offer 7.5 frames/sec
vs 30 (real time) or even less depending on the amount of pixels you need
to process. If you wish to do real time imaging 30fps, 7.5fps will not
do. Also for fluorescence the shortest duration exposure is preferred.
* The camera has to have a good performance to cost ratio. At the low end
there are plenty of security type cameras out there, but will they offer
you the performance you require.
* The big point here is that the higher the signal to noise, the quieter
the signal, the more gain that can be added to the signal/ which adds noise
as a result, but allows the user to get more sensitivity.
Thus if you start with the quietest / highest signal to noise obtainable
the better off you are when trying to get more gray levels with minimum
sensitivity or more signal to noise allows more gain to be added to achieve
a set sensitivity far superior to a signal to noise start point less than
the DVC.

The following features listed below are offered by the DVC camera line:

General Statement: Analog video RS-170 focus
*Most digitizer boards are typically 8 bits some go higher with digital
RS-422
input. With Dc's 10 bits of signal on the analog RS-170 video, this offers
first of all more top end room for adding more sensitivity and still
maintaining
8 bits/ 256 gray levels for the digitizer board.
*You will see more digitizer boards with 10 bit capability on the analog
RS-170
input. What will you use as a real time 30 frame / sec camera to match that
feature. With most ( real time ) cameras being in the 7 bit range, they
would
better qualify for security applications. There are some 8 bit cameras, but
they only offer 256 gray levels with not much room to add gain for more
sensitivity. The DVC camera line offers 10 bits or 1024 gray levels output
for those boards along with simultaneous digital RS-422 on the digital models
One of the digitizer boards with a 10 bit analog front end is Mutech. They
are the only one presently that has that capability for now and others will
follow soon.

* Very high signal to noise } 62dB at .5 lux at 30fps/ real time
* Frame transfer 1/2" format sensor with 755 horizontal by 484 vertical
pixels, or 565 TV lines horizontal by 350 TV lines vertical resolution.
* The only upgradable research camera with the researchers funding
limitations in mind using the same high quality sensor on all 3 models.
Upgradability from the RS-170 analog unit to the dual output 8 bit
digital or to the dual output 10 bit digital models with RS-170 analog.
(This allows the camera to grow with the researcher) and his budget.
See part 3 of 3 next.




From: DVCCO-at-aol.com
Date: Sun, 19 Mar 1995 03:52:28 -0500
Subject: Guide to Choosing A CCD Video Camera With New DVC Product Release

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And Digitizer Selection Guide For Benefit of Microscope Users Group

Monochrome Digital And Analog Video Camera Line By DVC Company
Part 1 of 3 due to capacity limitations of e-mail
*****************************************************************
With all due respect: If anyone is offended by a guide to choosing a
CCD video camera with a new product release that is
applicable for the users in this group ( HIT YOUR DELETE KEY NOW! )
There are some users that might appreciate this information.
(This was done with good intentions) so any providers of negative feedback
have been given the DELETE KEY OPTION and need not waste net time, thank you.
Valid questions are appreciated and I will try to answer them relative to
the volume I receive. Included is list of RS-422 digitizer boards in part 2
and 3.
* Please address any inquiry directly to dvcco-at-aol.com, not the main file
server.
*****************************************************************
Dear Microscope Users,

I wish to share some data with you on analog and digital monochrome cameras
that I thought you might be able to make use of when reviewing your present
or future camera situation. Some of the below information you might be aware
of, but others might not. If anyone belongs to other pertinent groups and
believes this information to be of benefit to that particular group we would
appreciate you letting us know of them.

Monochrome cameras offer much superior gray level and resolution performance
along with higher signal to noise specs than the typical color versions.
Everyone has different needs and the data below is for researchers who have
decided to utilize the benefits of monochrome cameras. Adding pseudo colors
to the many more gray levels attainable with a monochrome camera might be a
option for you, or adding a tunable liquid crystal filter in line with the
camera for serial Red, Green, Blue could also be a option if accurate
correlation of the pixel is important. For monochrome applications a
electronic tunable filter exists that is very selective with 5,7,10,15,20
,35 or 50nm band pass filters from 450 to 1050 nm range tunable
electronically!
See 2 of 2 for info on tunable filters.

Some things to look for in a research grade monochrome camera are the
following:
* The highest signal to noise possible in a real time 30 frames/sec. unit.
Signal to noise is the ultimate measure of a camera and no amount of
manipulation, bells and whistles, or 20 different knobs to adjust, will
change the bottom line which is the amount of gray levels obtainable.
* No geometric distortion- CCD cameras offer basically no distortion while
any tube camera gives you distortion in the } 5% range. My opinion is
that to use a tube camera where a ccd unit could be substituted is just
qualitative, not quantitative microscopy.
* No dead pixels on your sensor and lowest pixel variation with no false
fill-ins of pixels with data from the pixel next to it by use of memory
circuits due to imperfections in the manufacturing process.
Different types of CCD cameras have their uses.
The 2 main types are frame transfer (FT) and interline transfer (IT)
types for microscopy. CID types are also out there but have uses more in
machine vision applications.
Interline transfer sensors have large gaps between the pixels due to the
construction. They offer 100% fill factor by the use of plastic
micro lenses that help their sensitivity due to smaller well capacity,
but still have the ( same smaller wells.) Plastic does not offer good
performance down in the UV range below 400nm, and have other limitations
at the near IR range. 1/3 format sensors have even less well capacity
and I feel are only produced to provide better yields/profit for the
manufacturers for security applications.

Frame transfer sensors which DVC uses have dynamic range of 70dB to start
and have no dead pixels and no false fill-ins. The wells are very
large and the dark current is {30 electrons at 25C, room temperature.
The pixels are contiguous, or within angstroms of electrical separation.
Larger wells offer higher signal to noise. Double correlated sample and
hold circuits built right into the sensor help reduce noise further on
some frame transfer sensors. Frame transfer units offer one half the
vertical
resolution when doing integration and shuttering due the field transfer
technique used.
Spectral range with glass face plate attached 400-1100nm. Customers
doing (laser) work have reported response in the low 200nm range with
face plate removed and up to 1300nm.

Typical bit vs signal to noise ranges:
7 bits = 44dB to technically 50dB but more like 54dB realistically
8 bits = 55dB to 61dB or 256 gray levels
10 bits = 62dB+ for 1024 gray levels. **10 bits offers 4 times the
performance of an 8 bit camera.
* Another words a camera specification of 50dB falls into the 7 bit range
even though manufacturers and sales people might have you believe
otherwise. If you have an 8 bit board why not use a 8 bit or higher
camera.





From: DVCCO-at-aol.com
Date: Sun, 19 Mar 1995 03:54:55 -0500
Subject: Video Camera Selection Guide3of3

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Video Camera Selection Guide and DVC Product Release 3 of 3

* Sensitivities in the 1x10-4fc range at over 50% video. When other (IT)
type cameras barely see an image these DVC units are offering a perfect
image with extra gain to spare and no vertical line noise. Estimate
performance at about 5-6 ND filter better sensitivity than 768 type
interline transfer sensors.
* The only 10 bit digital camera with dual outputs RS-170 & RS-422 and at
real time video rates! 30 frames per second
* The only camera that has no sensor board. Sensor is physically mounted
to the front plate of the camera. Cooling above dew point can then be
done to have a truly quantitative camera in that temperature of sensor
can be lowered from room temperature or regulated at a constant
temperature or our -40C unit can be added.
* No need to cool the camera in most applications/ save the money.
* The DVC camera line was designed from the ground up to be a digital
output camera not a security camera. We use linear well regulated
multiple voltage linear power supply and solid C-Mounts with a 360
degree compression ring holding the c-ring firmly in place, not just a
little set screw that tends to loosen with time and strip out the
threads.
* 30dB of additional gain can be added for manual internal adjustable
operation accessible via a port from outside the camera, or external gain
and offset, or computer controlled gain! 30dB gain vs the others at 20dB
because the } 62db performance can fully utilize the 30db range. Where
with more than 20db on the IT types you just might end up with snow.
* Many other modifications can be done and our units do not come with AGC
unless you specify automatics. Automatics are for security cameras.
DVC will be responsive to your requirements from the end user to the OEM.
* Glass face plate removal to allow the user to easily go down to 300nm or
so. Customers with laser applications have reported response in the low
200nm range obviously at low quantum efficiencies.

The DVC camera line is excellent for any high signal to noise or low light
application and in real time!

DVC fits a niche between the too pricey and slow non real time digital only
video cameras and the security type cameras which don't offer enough of what
you need. If you like the resolution DVC can offer our flexibility in being
able to upgrade to the digital models is nice to have.
If any of you are interested in having a digital camera that works with your
Silicon Graphics Indy /Indigo 2 or Sun system, let us know.
DVC cameras are made in the USA and have a two year warranty.

* Three models of monochrome camera which are all upgradable to the DVC-10.
DVC-0A - RS-170 only with DB-37 connector for remote gain and upgradable
to the digital output RS-422 versions offering both
outputs.
DVC-8 - 8 bit RS-422 digital with RS-170 simultaneously.
DVC-10 -10 bit RS-422 digital with RS-170 simultaneously.

*** Below you will find a list of RS-422 digitizer boards that are compatible
to the DVC camera line, others are in the works.

****** Compatible Digitizers for RS-170 analog monochrome video are any
manufacturer. RS-170 is standard, typically from a BNC connector.
Does your board have capability to process RS-170 at 10 bits?
The DVC analog output will offer this. Remember if running 10 bit analog
RS-170 to a 8 bit board you will only see 8 bits.

Some of these boards have either 8 bit or 10 bit and higher capability.
*Compatible digitizers for RS-422 digital video that are
plug and play with the DVC digital camera line are listed below:

Manufacturer A-Z Platform Board Name/Model
Alacron IBM Alacron
Bit Flow IBM/MAC Raptor-VL
Coreco IBM F-64, OC-500
Datacube IBM MV-200
Dipix IBM P-360, XPG-1000
Epix IBM 4 Meg, Model 12
EDT SUN S-bus EDT
Hyperspeed IBM Hyperspeed
Imaging Technology IBM 15040, AFG, MFG
Imagraph IBM HI-Def
Matrox IBM Magic, 1280, 640, L/C
MuTech IBM 10 bit analog video in
Precision Digital (PDI) IBM/MAC IMAXX-PCI, SD
Univision IBM Piranha

Let DVC know if you wish, the following information:
Which digitizer board you are using or would like to use, or need help on.
If you have interest in the PCI bus
IBM, MAC, Sun, or SGI Indy or Indigo2 computer used
If DVC can help answer some of your technical concerns.
If your interest is analog or digital or both
If your interest is in tunable electronically selectable filters call DVC.

*****************************************************************************
***New DVC Camera Release estimated 4/95 will be the following:
Same features as above except now the digital RS-422 can be programmed or
reprogrammed for the VINO digital bus on the
((( SGI- Silicon Graphics Indy/Indigo 2 workstations)))
Imagine being able to move from SGI to RS-422 digital compatibility
with simultaneous RS-170 analog video!
Info on our cameras will be on the SGI Web soon.
*****************************************************************************

Feel free to e-mail, fax, or call DVC for more information.
If DVC can not reach you, we can not help you, so we chose to put this info
on
the net in hopes of helping anyone that needs it with a cost effective
research
grade video camera product that we feel, has a definite focus to this
microscope group. DVC has heard many of your problems and can be helpful,
and
possibly offer a solution. Stay in touch.

Sincerely,

Richard Klotsche
DVC Company
e-mail: dvcco-at-aol.com
phone: (619) 444-8300
fax: (619) 444-8321









From: m.dickson-at-unsw.edu.au (Melvyn R. Dickson)
Date: Mon, 20 Mar 1995 14:44:14
Subject: Re: Int'l MAS Conf??

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In article {n1416923531.369-at-mse.engin.umich.edu} "Wil Bigelow" {Wil_Bigelow-at-mse.engin.umich.edu} writes:
} Date: 14 Mar 1995 16:31:43 -0400
} From: "Wil Bigelow" {Wil_Bigelow-at-mse.engin.umich.edu}
} Subject: Int'l MAS Conf??

} Subject: Time: 4:23 PM
} OFFICE MEMO Int'l MAS Conf?? Date: 3/14/95

} I have heard rumors that there will be an international microbeam analysis
} meeting in Australia early in 1996. Does anyone know if
} this is so; and if it is, do you have any information about when and
} where it will be?

The meeting is the first meeting of the International Union of Microbeam
Analysis Societies (AKA IUMAS).

It will be held at the University of Sydney, Sydney, NSW Australia for Feb 5
to Feb 9 1996 (Preceeded by workshops) in conjunction with our 14th E.M.
Conference and 9th LM symposium. All welcome. Warm weather, water, cool
beer. David Cockayne is the chair.

Information on the WWW at http://www.bio.uts.edu.au/em.html





From: kris-at-miat0.vein.hu
Date: Fri, 17 Mar 1995 21:08:19
Subject: Subscription request

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From: Calidris-at-ett.se (George Farrants)
Date: 20 Mar 1995 08:38:54 GMT
Subject: Digitising TEM negatives

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Digitising images

For low cost, high capacity, high resolution and high quality,
_nothing_ beats film!

ÒUnfortunatelyÓ if you want to do digital image processing on
micrographs then they must be digitised. As previous answers have
indicated there is a choice between flat bed scanners and CCD
cameras. ThereÕs no doubt that the flatbed scanner produces better
data, but the cost is very high. (In addition to purchase price you have
maintenance, and so on.). Bill TivolÕs first reply overestimated the
cost of the CCD camera solution. You can get away with well under
$10000 for camera, lens, stand and light box, a tenth of the price for
flat bed scanners.

Optronics made a rotating drum scanner some years ago, but I think
itÕs disappeared now. Does anyone know? What did/does it cost?

The Ektron scanner mentioned by Larry Allard is new to me. Does the
referenced article give details? Who makes it?

Lab quality CCD cameras often have a geometric distortion between x
and y directions of a few percent. This must be measured and
corrected for in software. Another limitation is nonlinearity of the
response with OD, if you are planning on intensity measurements.
Again, it must be measured (with a stepwedge of standard ODs) and
corrected for.

CCD cameras have problems with ED patterns, as Bill also pointed
out. The very sharp spots with very high gradients cause trouble. I
know of some people who are using CCD camera for ED digitising,
maybe they can comment in the List about performance.

Another solution is to miss out film and have the CCD camera on the
microscope. Horribly expensive, but it has certain advantages. Is
anyone digitising ED patterns in this way, and what sort of
performance are they getting? I know of one group who is doing so,
with success. Are the specifications of on-line CCD cameras so much
better than lab ones?

As my old dad used to say ÒYou get what you pay forÓ when
confronted with The Sun newspaper at 20p and The Times, at 30p.

Yours,
George Farrants






From: sje-at-po.CWRU.Edu (Steven J. Eppell)
Date: Mon, 20 Mar 1995 10:13:55 -0500
Subject: networking a printer

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Message-Id: {199503201513.KAA19483-at-robin.INS.CWRU.Edu}


I would like to thank everyone that replied to my query concerning
networking a Tektronix Phaser IISDX dye sublimation printer.
Following is a summary of the problem and the responses.

I wished to put the printer on a Novell network so that it could be
accessed from macs, PCs, and Unix boxes. I had purchased Tektronix's
Internal Ethertalk card (~$650).

I was told by several listserver users and multiple people at Tektronix
that this was absolutely impossible to network the printer under a Novell
server using the Ethertalk card. Tektronix put me on to a company in
California called Milan that makes a network interface box that should
have allowed me to use the parallel port of the printer as the network
port. The cost of this box was ~$850.

I was told by several listserver users that it was trivial, no problem, plug
it in and it works regarding hooking the printer up to the network.

Finally, I convinced my network administrator to set up a que on one of
the network servers and to activate a faceplate so that I could connect
my printer to the net. The result .... Novell works fine with Tektronix's
Ethertalk card. I have printed from all three platforms and they all work.

The moral of the story: Keep asking your question until you get the
answer you want to hear!

p.s. For all of those highly experienced network users who told me in no
uncertain terms that I was a fool for not checking with my network
administrator before purchasing the printer .... Our local network here
has, conservatively speaking, 5,000 users. The network administration
is organized with a flowchart of personnel that would make the Ex-
Soviet politburo blush. I did try to go through logical channels before
making the purchase and was told, "we won't guarantee that it will work,
the best we can offer is for you to buy the printer and we'll test it out
when it gets here." I suppose this is probably an indication of what the
future of networking will be (once everybody's LAN gets so huge).




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Mon, 20 Mar 1995 17:08:58 -0500 (EST)
Subject: CCD Guide

Contents Retrieved from Microscopy Listserver Archives
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Sorry for wasting the bandwidth, but last week a 3 part information guide
on a particular ccd camera was posted to this forum or the confocal
microscopy list server. A disk crash lost my
copy of this posting as well as the address of the sender before I had a
chance to review it. If the sender could repost a copy directly to me, I
would appreciate it.
Again, sorry for cluttering your mailbox.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 20 Mar 1995 18:21:36 -0800 (PST)
Subject: Re: embedding media & thick sections .....

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X-Sender: glenmac-at-homer07.u.washington.edu

Well, I was cleaning my email folders on the mainframe and came across
this postponed message. Here it is, hope it can still be useful.

Regards,
Glen


There is always celloidin, its disadvantages are a lengthy embedding
process. It produces a lot of shrinkage in animal tissue, the plant
cell wall should resist gross shrinkage, but there may be vacuolization.

For material that has been stained prior to embedding you might try
embedding in an epoxy like Pelco's EMBed. You can cut up to 50 microns
by either a carbide tungsten knive or using a standard steel knife.
These are real knives, not those disposable blades. You also need a
solidly built microtome.
The carbide knife only needs a slow cutting stroke. The steel knife
needs an extremely slow stroke or you can watch the metal forming the
knife's edge be ripped away before your very eyes. A small tacking iron,
like those used for dry mounting photographs, can be clamped on the end of
the knife with a C-clamp. Heat the knife to about 50 deg. C and the
plastic will cut more easily.

Glen MacDonald
Hearing Development Laboratories RL-30
University of Washington
Seattle, WA 98195
(206)543-8360
glenmac-at-u.washington.edu


On Tue, 8 Nov 1994, Mike Folsom wrote:

} Folks -
}
} A quick question -
}
} I'm trying to embed plant material is some type of matrix that will
} allow me to make fairly thick sections, say 25 - 50 um.
}
} I've used paraffin and the tissue starts to fracture when section
} thickness gets greater than 20 - 25 um. Frankly besides embedding
} the tissue in plastic and then grinding it down I'm not sure
} what else to do.
}
} I'd appreciate any suggestions -
}
} Michael
}
} _______________________________________________________________________________
} M.W.Folsom/Biology/UNM/Albuquerque,NM~87131/505.277.4277/mwfolsom-at-hydra.unm.edu
}
}





From: Stuart.McClure-at-adl.soils.csiro.au (Stuart McClure)
Date: Tue, 21 Mar 1995 14:20:01 +0900
Subject: Video Camera Selection Guide1of3

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Message-Id: {199503210352.AA02291-at-shrike.adl.soils.csiro.au}
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Jay Jerome {jjerome-at-isnet.is.wfu.edu}

} Date: Sun, 19 Mar 1995 03:52:28 -0500
} From: DVCCO-at-aol.com
} To: Microscopy-at-aaem.amc.anl.gov
} Subject: Video Camera Selection Guide1of3
}
} Subject: Guide to Choosing A CCD Video Camera With New DVC Product Release
} And Digitizer Selection Guide For Benefit of Microscope Users Group
}
} Monochrome Digital And Analog Video Camera Line By DVC Company
} Part 1 of 3 due to capacity limitations of e-mail
} *****************************************************************
} With all due respect: If anyone is offended by a guide to choosing a
} CCD video camera with a new product release that is
} applicable for the users in this group ( HIT YOUR DELETE KEY NOW! )
} There are some users that might appreciate this information.
} (This was done with good intentions) so any providers of negative feedback
} have been given the DELETE KEY OPTION and need not waste net time, thank you.
} Valid questions are appreciated and I will try to answer them relative to
} the volume I receive. Included is list of RS-422 digitizer boards in part 2
} and 3.
} * Please address any inquiry directly to dvcco-at-aol.com, not the main file
} server.
} *****************************************************************
} Dear Microscope Users,
}
} I wish to share some data with you on analog and digital monochrome cameras
} that I thought you might be able to make use of when reviewing your present
} or future camera situation. Some of the below information you might be aware
} of, but others might not. If anyone belongs to other pertinent groups and
} believes this information to be of benefit to that particular group we would
} appreciate you letting us know of them.
}
} Monochrome cameras offer much superior gray level and resolution performance
} along with higher signal to noise specs than the typical color versions.
} Everyone has different needs and the data below is for researchers who have
} decided to utilize the benefits of monochrome cameras. Adding pseudo colors
} to the many more gray levels attainable with a monochrome camera might be a
} option for you, or adding a tunable liquid crystal filter in line with the
} camera for serial Red, Green, Blue could also be a option if accurate
} correlation of the pixel is important. For monochrome applications a
} electronic tunable filter exists that is very selective with 5,7,10,15,20
} ,35 or 50nm band pass filters from 450 to 1050 nm range tunable
} electronically!
} See 2 of 2 for info on tunable filters.
}
} Some things to look for in a research grade monochrome camera are the
} following:
} * The highest signal to noise possible in a real time 30 frames/sec. unit.
} Signal to noise is the ultimate measure of a camera and no amount of
} manipulation, bells and whistles, or 20 different knobs to adjust, will
} change the bottom line which is the amount of gray levels obtainable.
} * No geometric distortion- CCD cameras offer basically no distortion while
} any tube camera gives you distortion in the } 5% range. My opinion is
} that to use a tube camera where a ccd unit could be substituted is just
} qualitative, not quantitative microscopy.
} * No dead pixels on your sensor and lowest pixel variation with no false
} fill-ins of pixels with data from the pixel next to it by use of memory
} circuits due to imperfections in the manufacturing process.
} Different types of CCD cameras have their uses.
} The 2 main types are frame transfer (FT) and interline transfer (IT)
} types for microscopy. CID types are also out there but have uses more in
} machine vision applications.
} Interline transfer sensors have large gaps between the pixels due to the
} construction. They offer 100% fill factor by the use of plastic
} micro lenses that help their sensitivity due to smaller well capacity,
} but still have the ( same smaller wells.) Plastic does not offer good
} performance down in the UV range below 400nm, and have other limitations
} at the near IR range. 1/3 format sensors have even less well capacity
} and I feel are only produced to provide better yields/profit for the
} manufacturers for security applications.
}
} Frame transfer sensors which DVC uses have dynamic range of 70dB to start
} and have no dead pixels and no false fill-ins. The wells are very
} large and the dark current is {30 electrons at 25C, room temperature.
} The pixels are contiguous, or within angstroms of electrical separation.
} Larger wells offer higher signal to noise. Double correlated sample and
} hold circuits built right into the sensor help reduce noise further on
} some frame transfer sensors. Frame transfer units offer one half the
} vertical
} resolution when doing integration and shuttering due the field transfer
} technique used.
} Spectral range with glass face plate attached 400-1100nm. Customers
} doing (laser) work have reported response in the low 200nm range with
} face plate removed and up to 1300nm.
}
} Typical bit vs signal to noise ranges:
} 7 bits = 44dB to technically 50dB but more like 54dB realistically
} 8 bits = 55dB to 61dB or 256 gray levels
} 10 bits = 62dB+ for 1024 gray levels. **10 bits offers 4 times the
} performance of an 8 bit camera.
} * Another words a camera specification of 50dB falls into the 7 bit range
} even though manufacturers and sales people might have you believe
} otherwise. If you have an 8 bit board why not use a 8 bit or higher
} camera.
}
}
}
---------------------------------------------------------------------------
Stuart G. McClure, | Post small : P.B.#2, Glen Osmond,
CSIRO Division of Soils, | Sth Australia, AUSTRALIA, 5064.
Adelaide Laboratories, | Post large : Waite Rd, Urrbrae,
SA, Australia | Sth Australia, AUSTRALIA, 5064.

Phone: (08) 303-8484 International use +61-8- instead of (08)
Fax: (08) 303-8550
Email: Stuart.McClure-at-adl.soils.csiro.au
---------------------------------------------------------------------------





From: Stuart.McClure-at-adl.soils.csiro.au (Stuart McClure)
Date: Tue, 21 Mar 1995 14:20:13 +0900
Subject: Video Camera Selection Guide2of3

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199503210352.AA02295-at-shrike.adl.soils.csiro.au}
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Jay Jerome {jjerome-at-isnet.is.wfu.edu}

} Date: Sun, 19 Mar 1995 03:54:20 -0500
} From: DVCCO-at-aol.com
} To: Microscopy-at-aaem.amc.anl.gov
} Subject: Video Camera Selection Guide2of3
}
} DVC Video Camera Guide And Release 2 of 2
}
} Remember, if the camera is on the ragged edge of 8 bit performance and
} you want to add gain for more sensitivity, even the smallest amount of
} gain might put you in the 7 bit range if you we not there already.
} If you have 10 bit capability on your board why not use a 10 bit camera.
} * Also a camera specification of 60dB is not a 10 bit camera only an 8.
} * Also there are many types of noise in a sensor besides thermal noise.
} Cooling a sensor will reduce (thermal noise only) and allow you to
} integrate longer. I would double check the impression some make that
} when a 50db sensor is cooled it offers a better s/n of 10 or 12 bits vs
} just 7.
} The reduction of one type of noise (thermal) is all that cooling
} provides.
} * Other types of sensor noise besides dark current include photon, nyquest,
} shot, gain noise, and flicker noise etc.
} * The signal to noise of the sensor at room temperature is a good measure
} of what you can expect.
} ** Look at the quantum efficiency at the nm range needed.
} ** look at the spectral response of the sensor.
} ** There are different grades of sensors available relative to dead
} pixels and gray level variation on other manufacturers cameras.
} *Digital RS-422 eliminates pixel jitter for sub-pixel accuracy if it is
} needs versus the genlock action between the digitizer board and the
} camera as they try to lock to each other in the RS-170 analog mode.
} *Digital cameras of the higher end variety typically offer 7.5 frames/sec
} vs 30 (real time) or even less depending on the amount of pixels you need
} to process. If you wish to do real time imaging 30fps, 7.5fps will not
} do. Also for fluorescence the shortest duration exposure is preferred.
} * The camera has to have a good performance to cost ratio. At the low end
} there are plenty of security type cameras out there, but will they offer
} you the performance you require.
} * The big point here is that the higher the signal to noise, the quieter
} the signal, the more gain that can be added to the signal/ which adds noise
} as a result, but allows the user to get more sensitivity.
} Thus if you start with the quietest / highest signal to noise obtainable
} the better off you are when trying to get more gray levels with minimum
} sensitivity or more signal to noise allows more gain to be added to achieve
} a set sensitivity far superior to a signal to noise start point less than
} the DVC.
}
} The following features listed below are offered by the DVC camera line:
}
} General Statement: Analog video RS-170 focus
} *Most digitizer boards are typically 8 bits some go higher with digital
} RS-422
} input. With Dc's 10 bits of signal on the analog RS-170 video, this offers
} first of all more top end room for adding more sensitivity and still
} maintaining
} 8 bits/ 256 gray levels for the digitizer board.
} *You will see more digitizer boards with 10 bit capability on the analog
} RS-170
} input. What will you use as a real time 30 frame / sec camera to match that
} feature. With most ( real time ) cameras being in the 7 bit range, they
} would
} better qualify for security applications. There are some 8 bit cameras, but
} they only offer 256 gray levels with not much room to add gain for more
} sensitivity. The DVC camera line offers 10 bits or 1024 gray levels output
} for those boards along with simultaneous digital RS-422 on the digital models
} One of the digitizer boards with a 10 bit analog front end is Mutech. They
} are the only one presently that has that capability for now and others will
} follow soon.
}
} * Very high signal to noise } 62dB at .5 lux at 30fps/ real time
} * Frame transfer 1/2" format sensor with 755 horizontal by 484 vertical
} pixels, or 565 TV lines horizontal by 350 TV lines vertical resolution.
} * The only upgradable research camera with the researchers funding
} limitations in mind using the same high quality sensor on all 3 models.
} Upgradability from the RS-170 analog unit to the dual output 8 bit
} digital or to the dual output 10 bit digital models with RS-170 analog.
} (This allows the camera to grow with the researcher) and his budget.
} See part 3 of 3 next.
}
}
---------------------------------------------------------------------------
Stuart G. McClure, | Post small : P.B.#2, Glen Osmond,
CSIRO Division of Soils, | Sth Australia, AUSTRALIA, 5064.
Adelaide Laboratories, | Post large : Waite Rd, Urrbrae,
SA, Australia | Sth Australia, AUSTRALIA, 5064.

Phone: (08) 303-8484 International use +61-8- instead of (08)
Fax: (08) 303-8550
Email: Stuart.McClure-at-adl.soils.csiro.au
---------------------------------------------------------------------------





From: Stuart.McClure-at-adl.soils.csiro.au (Stuart McClure)
Date: Tue, 21 Mar 1995 14:20:17 +0900
Subject: Video Camera Selection Guide3of3

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199503210352.AA02299-at-shrike.adl.soils.csiro.au}
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Jay Jerome {jjerome-at-isnet.is.wfu.edu}

} Date: Sun, 19 Mar 1995 03:54:55 -0500
} From: DVCCO-at-aol.com
} To: Microscopy-at-aaem.amc.anl.gov
} Subject: Video Camera Selection Guide3of3
}
} Video Camera Selection Guide and DVC Product Release 3 of 3
}
} * Sensitivities in the 1x10-4fc range at over 50% video. When other (IT)
} type cameras barely see an image these DVC units are offering a perfect
} image with extra gain to spare and no vertical line noise. Estimate
} performance at about 5-6 ND filter better sensitivity than 768 type
} interline transfer sensors.
} * The only 10 bit digital camera with dual outputs RS-170 & RS-422 and at
} real time video rates! 30 frames per second
} * The only camera that has no sensor board. Sensor is physically mounted
} to the front plate of the camera. Cooling above dew point can then be
} done to have a truly quantitative camera in that temperature of sensor
} can be lowered from room temperature or regulated at a constant
} temperature or our -40C unit can be added.
} * No need to cool the camera in most applications/ save the money.
} * The DVC camera line was designed from the ground up to be a digital
} output camera not a security camera. We use linear well regulated
} multiple voltage linear power supply and solid C-Mounts with a 360
} degree compression ring holding the c-ring firmly in place, not just a
} little set screw that tends to loosen with time and strip out the
} threads.
} * 30dB of additional gain can be added for manual internal adjustable
} operation accessible via a port from outside the camera, or external gain
} and offset, or computer controlled gain! 30dB gain vs the others at 20dB
} because the } 62db performance can fully utilize the 30db range. Where
} with more than 20db on the IT types you just might end up with snow.
} * Many other modifications can be done and our units do not come with AGC
} unless you specify automatics. Automatics are for security cameras.
} DVC will be responsive to your requirements from the end user to the OEM.
} * Glass face plate removal to allow the user to easily go down to 300nm or
} so. Customers with laser applications have reported response in the low
} 200nm range obviously at low quantum efficiencies.
}
} The DVC camera line is excellent for any high signal to noise or low light
} application and in real time!
}
} DVC fits a niche between the too pricey and slow non real time digital only
} video cameras and the security type cameras which don't offer enough of what
} you need. If you like the resolution DVC can offer our flexibility in being
} able to upgrade to the digital models is nice to have.
} If any of you are interested in having a digital camera that works with your
} Silicon Graphics Indy /Indigo 2 or Sun system, let us know.
} DVC cameras are made in the USA and have a two year warranty.
}
} * Three models of monochrome camera which are all upgradable to the DVC-10.
} DVC-0A - RS-170 only with DB-37 connector for remote gain and upgradable
} to the digital output RS-422 versions offering both
} outputs.
} DVC-8 - 8 bit RS-422 digital with RS-170 simultaneously.
} DVC-10 -10 bit RS-422 digital with RS-170 simultaneously.
}
} *** Below you will find a list of RS-422 digitizer boards that are compatible
} to the DVC camera line, others are in the works.
}
} ****** Compatible Digitizers for RS-170 analog monochrome video are any
} manufacturer. RS-170 is standard, typically from a BNC connector.
} Does your board have capability to process RS-170 at 10 bits?
} The DVC analog output will offer this. Remember if running 10 bit analog
} RS-170 to a 8 bit board you will only see 8 bits.
}
} Some of these boards have either 8 bit or 10 bit and higher capability.
} *Compatible digitizers for RS-422 digital video that are
} plug and play with the DVC digital camera line are listed below:
}
} Manufacturer A-Z Platform Board Name/Model
} Alacron IBM Alacron
} Bit Flow IBM/MAC Raptor-VL
} Coreco IBM F-64, OC-500
} Datacube IBM MV-200
} Dipix IBM P-360, XPG-1000
} Epix IBM 4 Meg, Model 12
} EDT SUN S-bus EDT
} Hyperspeed IBM Hyperspeed
} Imaging Technology IBM 15040, AFG, MFG
} Imagraph IBM HI-Def
} Matrox IBM Magic, 1280, 640, L/C
} MuTech IBM 10 bit analog video in
} Precision Digital (PDI) IBM/MAC IMAXX-PCI, SD
} Univision IBM Piranha
}
} Let DVC know if you wish, the following information:
} Which digitizer board you are using or would like to use, or need help on.
} If you have interest in the PCI bus
} IBM, MAC, Sun, or SGI Indy or Indigo2 computer used
} If DVC can help answer some of your technical concerns.
} If your interest is analog or digital or both
} If your interest is in tunable electronically selectable filters call DVC.
}
} *****************************************************************************
} ***New DVC Camera Release estimated 4/95 will be the following:
} Same features as above except now the digital RS-422 can be programmed or
} reprogrammed for the VINO digital bus on the
} ((( SGI- Silicon Graphics Indy/Indigo 2 workstations)))
} Imagine being able to move from SGI to RS-422 digital compatibility
} with simultaneous RS-170 analog video!
} Info on our cameras will be on the SGI Web soon.
} *****************************************************************************
}
} Feel free to e-mail, fax, or call DVC for more information.
} If DVC can not reach you, we can not help you, so we chose to put this info
} on
} the net in hopes of helping anyone that needs it with a cost effective
} research
} grade video camera product that we feel, has a definite focus to this
} microscope group. DVC has heard many of your problems and can be helpful,
} and
} possibly offer a solution. Stay in touch.
}
} Sincerely,
}
} Richard Klotsche
} DVC Company
} e-mail: dvcco-at-aol.com
} phone: (619) 444-8300
} fax: (619) 444-8321
}
}
}
}
}
}
}
---------------------------------------------------------------------------
Stuart G. McClure, | Post small : P.B.#2, Glen Osmond,
CSIRO Division of Soils, | Sth Australia, AUSTRALIA, 5064.
Adelaide Laboratories, | Post large : Waite Rd, Urrbrae,
SA, Australia | Sth Australia, AUSTRALIA, 5064.

Phone: (08) 303-8484 International use +61-8- instead of (08)
Fax: (08) 303-8550
Email: Stuart.McClure-at-adl.soils.csiro.au
---------------------------------------------------------------------------





From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Tue, 21 Mar 1995 08:36:37 +0000 (GMT)
Subject: Re: embedding media & thick sections .....

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microscopy list {Microscopy-at-aaem.amc.anl.gov}

With reference to embedding plant material for thick(ish) sections. We
routinely use LR White (London Resin White acrylic resin for plant
material and can certainly cut sections up to 15-20um. The nice thing
about LR white is that is like the British, tolerant and kind to
specimens and you need only dehydrate to 80% EtOH and still get godd
embedding. You can use all sorts of light microscopy stains on sections
cut from such material.

Greetings from a beautiful spring morning in Cambridge where all the
daffodils are in full bloom along the Backs.

Patrick Echlin

Multi-Imaging Centre
School of Biological Sciences


On Mon, 20 Mar 1995, Glen
Macdonald wrote:

} Well, I was cleaning my email folders on the mainframe and came across
} this postponed message. Here it is, hope it can still be useful.
}
} Regards,
} Glen
}
}
} There is always celloidin, its disadvantages are a lengthy embedding
} process. It produces a lot of shrinkage in animal tissue, the plant
} cell wall should resist gross shrinkage, but there may be vacuolization.
}
} For material that has been stained prior to embedding you might try
} embedding in an epoxy like Pelco's EMBed. You can cut up to 50 microns
} by either a carbide tungsten knive or using a standard steel knife.
} These are real knives, not those disposable blades. You also need a
} solidly built microtome.
} The carbide knife only needs a slow cutting stroke. The steel knife
} needs an extremely slow stroke or you can watch the metal forming the
} knife's edge be ripped away before your very eyes. A small tacking iron,
} like those used for dry mounting photographs, can be clamped on the end of
} the knife with a C-clamp. Heat the knife to about 50 deg. C and the
} plastic will cut more easily.
}
} Glen MacDonald
} Hearing Development Laboratories RL-30
} University of Washington
} Seattle, WA 98195
} (206)543-8360
} glenmac-at-u.washington.edu
}
}
} On Tue, 8 Nov 1994, Mike Folsom wrote:
}
} } Folks -
} }
} } A quick question -
} }
} } I'm trying to embed plant material is some type of matrix that will
} } allow me to make fairly thick sections, say 25 - 50 um.
} }
} } I've used paraffin and the tissue starts to fracture when section
} } thickness gets greater than 20 - 25 um. Frankly besides embedding
} } the tissue in plastic and then grinding it down I'm not sure
} } what else to do.
} }
} } I'd appreciate any suggestions -
} }
} } Michael
} }
} } _______________________________________________________________________________
} } M.W.Folsom/Biology/UNM/Albuquerque,NM~87131/505.277.4277/mwfolsom-at-hydra.unm.edu
} }
} }
}
}




From: jyoung-at-nsctoronto.com (James Young)
Date: Tue, 21 Mar 1995 08:57:34 +0500
Subject: NEW Address

Contents Retrieved from Microscopy Listserver Archives
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cemerson-at-kean.ucs.mun.ca, dhoyle-at-tic.ab.ca, bray-at-hg.uleth.ca,
beebed-at-ere.umontreal.ca, tyerman-at-cliff.path.queensu.ca,
MICROSCOPY-at-AAEM.AMC.ANL.GOV, Eric Kokko {kokko-at-EM.AGR.CA} ,
jvyoung-at-inforamp.net, lburton-at-cc.UManitoba.CA, asmith-at-aps.uoguelph.ca

We have our own Domain now. My personal address
is jyoung-at-nsctoronto.com

General address for service info and parts and prices is service-at-nsctoronto.com





From: NoahHadas-at-aol.com
Date: Tue, 21 Mar 1995 09:58:53 -0500
Subject: Micro-manipulation newsgroup?

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

I'm a recent subscriber and am wondering if perhaps there is another
listserver that might be closer to my fields of interest, i.e.
micro-manipulation of cellular and sub-cellular particles either via mechanica
l or optical trapping techniques. If anyone knows of a group that is more
involved in these areas, I would appreciate if you could reply to me
directly.

Thanks,

Noah




From: Greg Begin :      GREGB-at-sales.lmt.com
Date: Tue, 21 Mar 1995 10:47:41 CST6CDT
Subject: Help finding Mr. Harold Kelly (Del if unknown)

Contents Retrieved from Microscopy Listserver Archives
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My appologies for clouding the Net with this traffic.

I have been attempting to get info to the following person,
if you can help, thank you in advance.

Person: Mr. Harold Kelly
address on the top of email: dy {hkelly-at-sgi73.wwb.noaa.gov

That is all I have on this person.

Thank you for your understanding and help on this.

Greg Begin - gregb-at-sales.LMT.com
3-21-95




From: greggk-at-acad.winthrop.edu
Date: Tue, 21 Mar 1995 12:52:05 -0500
Subject: Histology course idea

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WINTHROP UNIVERSITY Electronic Mail Message
Date: 21-Mar-1995 12:50pm EST
From: Kenneth Gregg
GREGGK
Dept: Biology
Tel No: 803-323-2111

TO: Remote Addressee ( _smtp%"microscopy-at-aaem.amc.anl.gov" )






WINTHROP UNIVERSITY Electronic Mail Message
Date: 21-Mar-1995 11:31am EST
From: Kenneth Gregg
GREGGK
Dept: Biology
Tel No: 803-323-2111

TO: Remote Addressee ( smtp%"microscopy-at-aaem.amc.anl.gov" )



Greetings,
My guess is that many members of the list teach a histology course at the
University level. I would like some comments on a method of teaching the
laboratory section of histology which we are considering. We find that many
students are rapidly bored when looking at tissue/organ sections in the light
microscope. They also seem to lack the ability to know when they have acquired
an acceptable level of expertise. As an antidote to this we are kicking around
the following idea -- and we would like to know if any members of the list have
tried similar approaches, and with what success?

We are thinking about having individual students prepare
computer-assisted-instruction lessons on topics such as epithelial tissues,
liver structure, etc. We would like to have the students prepare a hypertext
program for each topic. They would integrate the hypertext with captured images
from their light microscope observations as well as scanned diagrams and
electron micrographs which they could obtain from texts or photographs.
Students would be very much engaged in their own productions and could study
lessons prepared by other students. These programs could be used and improved
in subsequent years by other students. They could also be made available for
other institutions.

If you prefer, you may respond to me personally at
Greggk-at-Winthrop.edu

Thank you.





From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Tue, 21 Mar 1995 16:49:23 -0500 (EST)
Subject: query regarding light microscopy automation

Contents Retrieved from Microscopy Listserver Archives
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We are seriously interested in purchasing a setup for our Nikon Diaphot
that could be used for the following:
o quantitation of very low level fluorescence-- sensitive and linear
o but also useful for critical Nomarski applications such as microtubule
motility assays
o automation of filter wheels for multiple probes or fluorescence / phase
contrast image collection
o automation of stage moving, for instance for making mosaics automatically
or being able to move back to a specific field
o maybe Z motor and deconvolution, although the are not our main concerns
o ESSENTIAL: we are a multi-user facility so we need ease of operation

We are considering a Photometrics or Princeton Instruments cooled CCD camera.
We have looked at Oncor Imaging, I.P. Lab and have spoken to other
companies. For hardware, we have called companies that offer individual
parts (e.g. just the filter wheels) and, of course, have checked out Ludl.
We love NIH-Image, but want something does not require a large amount of
macro or other programming to get the hardware to work; basically, we want
plug and play.
Any suggestions or "stay away from this product at all costs" comments on
parts of systems or whole systems would be appreciated.
Thanks-
Michael Cammer
cammer-at-aecom.yu.edu






From: Weislaw Jablonski :      wis-at-lab.csl.utas.edu.au
Date: Wed, 22 Mar 1995 09:02:43 +1100
Subject: titanium in living tissue

Contents Retrieved from Microscopy Listserver Archives
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Hi there,
Could you help me with the problem of delineation of titanium in bone and
soft tissue.Any information and/or references would be a great help to me.
Study will involve the use of both SEM and EPMA, possibly FTIR as well.
Thank you all, Wis Jablonski , Email W.Jablonski-at-csl.utas.edu.au




From: Stephen A. Shaffer :      sshaffer-at-microdataware.com
Date: Saturday, April 1, 1995
Subject: San Francisco Micro. Soc. Meeting 4/1/95

Contents Retrieved from Microscopy Listserver Archives
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Place: Convene at Forensic Science Associates in Richmond,
Conclude at Oakland PD. (See contact information below for
reservations, maps, car pooling, and further details.)

Time: Convene by 10:00 am, Adjourn by 5:00 pm

Summary: This promises to be a most informative and interesting
meeting as we hear from some of the Bay Areas foremost
forensic microscopists, see some of the latest technology in
microscopy, and hear about some interesting forensic
applications of microscopy.

Agenda:

Forensic Science Associates, 10:00 am. Stephen A. Shaffer of
MicroDataware will introduce the topic of forensic science
in general and forensic microscopy in particular. Peter D.
Barnett of Forensic Science Associates will then discuss two
interesting cases involving forensic microscopy, and also
the role of the consulting (i.e., non-government)
microscopist in forensic cases. Pete will discuss cases
involving questions of ballot marking and alleged surgical
mistakes revealed by fibers in the nose! We'll conclude at
FSA by 11:30 or 11:45.

Bureau of Alcohol, Tobacco, and Firearms, 1:00 pm. After a brief
break for lunch at a Deli in Walnut Creek, we'll re-convene
at the ATF Laboratory where we will divide into two groups
to cover separate topics with two speakers. John Murdock
will explain and demonstrate the use of comparison
microscopy in firearms identification. John is the former
Director of the Contra Costa County Crime Laboratory and
also teaches criminalistics locally. He is an excellent
speaker and promises a fascinating discussion. Also at ATF,
we will meet with Robert Thompson who will demonstrate a new
technology for automated comparison microscopy. This
technology may revolutionize firearms work in criminalistics
laboratories by screening cases and suggesting most
promising comparisons for the human microscopist to perform.
We will adjourn from ATF by 2:30 to proceed to the Oakland
Police Department.

Oakland Police Department, 3:00 pm. At OPD, Diane Bowman will
discuss the use of microchemical testing procedures for the
identification of illicit drugs and narcotics, showing both
the utility and the beauty of these procedures for the rapid
identification of controlled substances. Mary Gibbons,
Director of the OPD Lab, will discuss a fascinating case
where microscopic particles of spray paint, identified and
compared by microscopy, were instrumental in unraveling a
suspect's story, leading to solution of a homicide. We will
aduourn from OPD by 5:00 pm.

At both ATF and OPD, each of our sub-groups will take turns with
each of the speakers so we all get to hear all of the
topics. Each topic will be covered in a presentation of
approximately 45 minutes duration.

Reservations Required!!! Because of the limited space available
within the microscopy labs we will visit, it may be
necessary to limit the number of people who attend. No more
than 20 can be accommodated so call Steve Shaffer ASAP to
reserve your space, receive maps to the locations, and to
coordinate with others on car pooling to the three sites we
will visit.

Contact: Stephen A. Shaffer at the numbers below. Don't delay,
call right away!

************************************************************
* Stephen A. Shaffer * Publishers of The Particle Atlas *
* MicroDataware * on CD-ROM. Developers of custom *
* 2894 Tribune Avenue * image and database software for *
* Hayward CA 94542-1637 * laboratories, specializing in *
* 1-510-582-6624 voice * light and electron microscopy. *
* 1-510-582-6624 fax * Email inquiries invited. *
* sshaffer-at-microdataware.com *
************************************************************





From: R.G.White-at-sci.monash.edu.au (Rosemary White)
Date: Wed, 22 Mar 1995 10:08:05 +1200
Subject: Re: embedding media & thick sections .....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Plant material can also be embedded in butyl methyl methacrylate and
sectioned up to 20 um. Stick the sections down to glass slides with
polyethylenimine. The resin is removed with acetone before antibody
staining, but you don't need to remove it if staining with something small
and water-soluble like aniline blue.

____________________________________________________________
Rosemary White __ /
Department of Ecology _/ \__/ \
and Evolutionary Biology / \
Monash University / Australia \
Clayton, Victoria 3168 \ ____ /
phone 61-3-905 5670 \_/ \_*_/
fax 61-3-905 5613 __
email r.g.white-at-sci.monash.edu.au \/





From: Kris_Kavanau-at-dmcmail.ucsf.edu
Date: Tue, 21 Mar 1995 15:17:09 PST
Subject: Usage "Tracking" Programs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {1995Mar21.151709.2878643829-at-dmcmail.ucsf.edu}
To: microscopy-at-aaem.amc.anl.gov (microscopy)

Subject: Time: 2:10 PM
OFFICE MEMO Usage "Tracking" Programs Date: 3/21/95
Hi everybody!
Does anyone have any usage tracking programs, or know where they might be
obtained? I am especially interested in a DOS program that is compatible
with Meridian software. Does anyone have any experience using tracking
programs? I am also interested in programs that are compatible with HP
and Macintosh acquisition/analysis software for flow cytometers (primarily
Becton Dickinson.)
Any suggestions or comments would be greatly appreciated. Thank you very
much!

__________________________________________________________
Kris Kavanau Internet:
kavanau-at-dmc.ucsf.edu
Manager, Lab for Cell Analysis Telephone: 415-476-2631
Division of Molecular Cytometry FAX: 415-476-8218
University of California
San Francisco, CA 94143
__________________________________________________________








From: Charles Garber
Date: Wed, 22 Mar 1995 07:43:48 EST
Subject: Titanium in living tissue [Question from Weislaw Jablonski on 3/21]

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Charles A. Garber, PH. D. * EMC.Ver #2.10P ] --

SPI Supplies

For TEM examination, we using the following procedure:

1] Pick up the unstained sections on a silicon dioxide coated TEM grid,

2] Gently etch (e.g. 10 seconds) the now supported section in a barrel
geometry reactive etcher (for example, the SPI Model Plasma Prep II)
using oxygen as the etching gas, which will immediately etch away all
traces of the organic matrix, leaving the inorganics dispersed, in
their original locations, on the silicon dioxide support film. Usually
these residues form a ghost type of outline of original cell shapes. I
don't know what bone might look like if etched this way.

The reason for the silicon dioxide support film is that it will not be
etched by the oxygen plasma, where as any kind of organic or
carbonaceous support film would otherwise be etched away.

The reason for the etching to remove the organic part of the sample is
to reduce to almost zero the Bremstrahlung radiation, thereby
increasing greatly the lower detection limits.

3] We have always found the TEM to be better for doing this kind of
analysis than SEM/EDS, mainly because of the higher resolution
possibilities.


Let me know if you would need further information. The making of the
silicon dioxide coated grids requires a bit of art but they are not all
that difficult to make, so long as you are patient.

Charles A. Garber, Ph. D.
PRESIDENT
SPI SUPPLIES
PO Box 656
West Chester, PA 19381-0656 USA

Ph: 1-(610)-436-5400
FAX: 1-(610)-436-5755
e-mail: sp-supp-at-cerf.net






From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Wed, 22 Mar 1995 09:32:57 -0500
Subject: EM TECH POSITION

Contents Retrieved from Microscopy Listserver Archives
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The following position is available at the Johns Hopkins U. School of Medicine:

Electron Micrscopy Technician/19 hrs/wk PART-TIME:

Position will be responsible for semi- and ultra-thin sectioning on
glass and diamond knives. Standard grid prep and staining of sections;
maintenance
of electron gun parts (polishing cleaning etc.). Replenising of photo- and lab
chemical stocks, care of peripheral equipment and other related duties.
College degree, BS or equivalent and minimum two years experience.
Special
skills/knowledge of diamond knive use and maintenance, routine EM lab
operations.

All interested candidates should contact:
Lou Cote
(410) 955-0519
Job # M.3531.94

or

Mike Delannoy
(410) 955-1365





From: Maggy Piranian :      maggy-at-sparky2.esd.mun.ca
Date: Wed, 22 Mar 1995 13:31:51 -0330 (NST)
Subject: Re: Usage "Tracking" Programs

Contents Retrieved from Microscopy Listserver Archives
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Kris et al,
I use DIRECT ACCESS from Fifth Generation Systems. It keeps track of the
time, reqires a pssword and has the option of also requiring a project name.
This forces the issue with people who think it's better for me to chase
down their grant number then for them to remember, and makes billing much
easier. We also keep a log book for people to record problems and i modify
billings accordingly. When I came here this was already installed on the
XRD computer so I did no comparative shopping, & I don't know what else
is out there. Their address is
FIFTH GENERATION SYSTEMS INC
10049 n. Reiger Rd
Baton Rouge LA 70809
(504) 291-7221

Maggy Piranian
Memorial U of Newfoundland

On Tue, 21 Mar 1995 Kris_Kavanau-at-dmcmail.ucsf.edu wrote:

} Subject: Time: 2:10 PM
} OFFICE MEMO Usage "Tracking" Programs Date: 3/21/95
} Hi everybody!
} Does anyone have any usage tracking programs, or know where they might be
} obtained? I am especially interested in a DOS program that is compatible
} with Meridian software. Does anyone have any experience using tracking
} programs? I am also interested in programs that are compatible with HP
} and Macintosh acquisition/analysis software for flow cytometers (primarily
} Becton Dickinson.)
} Any suggestions or comments would be greatly appreciated. Thank you very
} much!
}
} __________________________________________________________
} Kris Kavanau Internet:
} kavanau-at-dmc.ucsf.edu
} Manager, Lab for Cell Analysis Telephone: 415-476-2631
} Division of Molecular Cytometry FAX: 415-476-8218
} University of California
} San Francisco, CA 94143
} __________________________________________________________
}
}
}
}
}




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Wed, 22 Mar 1995 11:30:37 -0600 (CST)
Subject: Sad News for the MSA Community

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Dear Colleagues,

We regrettably learned Monday, of the untimely death this last
Saturday of Mort Maser an individual whose efforts and contributions
to the Microscopy Community here in the US are well known and
valued by many of us.

Mort will be certainly missed and may I speak for the entire MSA
family in expressing our condolences to his family and friends.
For those of you who are interested I have gotten some details
concerning a memorial for Mort and detail those below.

..Nestor

-----------------------------------------------------------

In memory of Mort, his family is establishing the Morton D Maser
Scholarship Fund. Contributions can be sent to: Larry Maser,
P.O. Box EM, Woods Hole, MA 02543. Please designate your gift
to the Morton D Maser Scholarship Fund.

Memorial services celebrating Mort's life are being planned for the
summer months. Details will be announced as they are finalized.

---------------------------------------------------------------

Morton D "Mort" Maser, President of Woods Hole Educational
Associates, and Executive Secretary to Council of both the
Microscopy Society of America and the Histochemical
Society, died on Saturday, March 18, 1995 at the age of 60.

He earned his A.B. from the University of Pennsylvania in 1955,
and his Ph.D. in biophysics from the University of Pittsburgh in
1962. He held research and faculty positions at the Mellon
Institute, Harvard University, the Millard Fillmore Hospital,
Northeastern University, Creighton University, and the Marine
Biological Laboratory.

He developed roughly 100 short courses in electron microscopy and
related fields for scientists and technicians, and made more than
50 contributions to peer-reviewed publications on electron
microsopy and related topics.

He was a member of several professional societies including
serving as Chair of EMSA's Education Committee; past President,
Director of the New England Society for Electron Microscopy; the
American Association for the Advancement of Science; American
Society of Cell Biology; and the International Society for
Stereology.

His research has spanned the sciences from electron microscopical
techniques, to computer sciences, to software systems.

He is survived by his wife, Naomi, children Larry of Forestdale,
Massachusetts and Jill, of Philadelphia, and grandson, Benjamin.

-------------------





From: fskarl-at-goodyear.com (Frank Karl)
Date: Wed, 22 Mar 1995 13:06:15 -0500
Subject: Information wanted: Flatbed transparency scanners

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X-Sender: t456b15-at-rds163
Message-Id: {v01510101ab9615dcdabe-at-[163.243.13.93]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear Folks'

I was following the discussion on flatbed scanners with great interest, but
I need to find some vendors to request additional information. The
following units were mentioned: Umax PowerLink
AGFA Arcus
Perkin-Elmer
and
Optronics

Does any one have address, phone number or E-mail address for these or
other vendors?

Thanks for the assistance....Frank


Frank Karl fskarl-at-goodyear.com
Goodyear Tire & Rubber Co. Voice 216.796.7818
Analytical Services - Dept 415B Fax 216.796.3304
142 Goodyear Blvd
Akron, OH 44305
U.S.A.









From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Wed, 22 Mar 1995 14:41:51 -0400 (EDT)
Subject: RE: replacement for lactophenol

Contents Retrieved from Microscopy Listserver Archives
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X-NUPop-Charset: English

In message Mon, 20 Mar 95 14:23:11 EST, forbes-at-cip.org.ec writes:
We would greatly appreciate
} suggestions on other ways to clear and fix this tissue that do not
} involve lactophenol or other products which require special
} disposal facilities, which currently do not exist in Quito.
*******

We routinely use 1 M NaOH to clear flowers to check in vivo pollen growth
after Aniline Blue staining (for fluorescence). You might also want to try
a clearing protocol specifically designed for fungal infected leaf tissue
such as the one in the following publication:

AU HIGNIGHT-K-W. MUILENBURG-G-A. VAN-WIJK-A-J-P.

TI A CLEARING TECHNIQUE FOR DETECTING THE FUNGAL ENDOPHYTE ACREMONIUM- SP

IN GRASSES

SO BIOTECH HISTOCHEM

68 (2). 1993. 87-90.

JT BIOTECHNIC & HISTOCHEMISTRY.

KW FESTUCA-ARUNDINACEA FESTUCA-OVINA LOLIUM-PERENNE BRIGHT FIELD

MICROSCOPY ANALYTICAL METHOD

AB Leaf tissue of tall fescue Festuca arundinacea Schreb., hard fescue

Festuca ovina L., red fescue Festuca rubra L. and Perennial ryegrass

Lolium perenne L. was stained with rose Bengal or aniline blue to

detect the presence of the fungal endophyte Acremonium sp.. Specimens

were cleared using methyl salicylate, an optical clearing agent, and

viewed using bright field microscopy. Tissue was preserved as dried

tissue or stored in 70% aqueous ethyl alcohol before staining and

clearing. Tissue was observed at 2, 4 and 12 weeks following clearing

to check for stain retention. Staining with rose Bengal was inferior to

aniline blue when followed by the clearing agent methyl salicylate.

Fungal mycelia stained lighter with rose Bengal and were more difficult

to detect than mycelia stained with aniline blue. The results

illustrate the usefulness of combining staining and methyl salicylate

clearing for detecting fungal endophytes.

___________

Good Luck!

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: nina allen :      allen-at-wfu.edu
Date: Wed, 22 Mar 1995 16:56:34 -0500 (EST)
Subject: Re: query regarding light microscopy automation

Contents Retrieved from Microscopy Listserver Archives
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Have you tried calling Universal Imaging (you mentioned some of the other
competitive companies). They are at 610-344-9410. Their new metamorph
system will do just about everything you requested...and works well with
a cooled CCD.
Nina Allen




From: Strucural Biology Unit :      MICROSCOPY-at-SBSNOV1.AUCKLAND.AC.NZ
Date: Thu, 23 Mar 1995 10:03:42 GMT+1200
Subject: RE:35mm film in TEM

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} Date sent: Mon, 27 Feb 1995 09:30:43 -0500 (EST)
} From: YANGA-at-NCCCOT.AGR.CA
} Subject: RE:35mm film in TEM
} To: microscopy-at-aaem.amc.anl.gov

} We use Eastman motion picture film (5302 fine grain release
} positive film) in Philips EM300 in the past and currently in
} Zeiss EM902 without any problem.
}
} Ann Fook Yang

We use copex PET10 in our CM12 and 301. It doesn't outgase much, is
very thin so is easy to handle and has not sprocket hole to interfere
with the images (depending onthe format).

Terry





From: fskarl
Date: Wednesday, March 22, 1995 1:06PM
Subject: Information wanted: Flatbed transparency scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Folks'

I was following the discussion on flatbed scanners with great interest, but
I need to find some vendors to request additional information. The
following units were mentioned: Umax PowerLink
AGFA Arcus
Perkin-Elmer
and
Optronics

Does any one have address, phone number or E-mail address for these or
other vendors?

Thanks for the assistance....Frank


Frank Karl fskarl-at-goodyear.com
Goodyear Tire & Rubber Co. Voice 216.796.7818
Analytical Services - Dept 415B Fax 216.796.3304
142 Goodyear Blvd
Akron, OH 44305
U.S.A.









From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Thu, 23 Mar 1995 09:46:25 -0500
Subject: EM TECH POSITION

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} To: microscopy-at-aaem.amc.anl.gov
} From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
} Subject: EM TECH POSITION
}
} The following position is available at the Johns Hopkins U. School of Medicine:
}
} Electron Micrscopy Technician/19 hrs/wk PART-TIME:
}
} Position will be responsible for semi- and ultra-thin sectioning on
} glass and diamond knives. Standard grid prep and staining of sections;
maintenance
} of electron gun parts (polishing cleaning etc.). Replenising of photo- and lab
} chemical stocks, care of peripheral equipment and other related duties.
} College degree, BS or equivalent and minimum two years experience.
Special
} skills/knowledge of diamond knive use and maintenance, routine EM lab
operations.
}
} All interested candidates should contact:
} Lou Cote
} (410) 955-0519
} Job # M.3531.94
}
} or
}
} Mike Delannoy
} (410) 955-1365
}





From: almonte-at-medcolpa.edu
Date: Thu, 23 Mar 1995 09:51:36 -0400
Subject: Cy5 &Cy3 background staining?

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Dear Friends,
As you all may remember, I was having crossing over problem between
FITC & Rhodamine. I followed the suggestion that some of you gave me and
used Cy5 (1.4 mg/ml) 1:100 and 1:400 and Cy3 (1.4 mg/ml) same dilution. I
did a control and I got a lot background staining. Any suggestion?
My next step will be to check my filters as some of you suggested.
Nichole Dutton suggested that before applying primaries antibodies
to block for nonspecifics with 20% horse serum solution, and use 1% bovine
serum albumin in my diluents. Nichole, I'd like further information on
this.
Dave, also suggested using different blocking reagent (BSA, milk,
gelatin, serum.) I'd like to learn more about this possibility, Dave.
Thanks,
Ciprian

__________________________________________________________
Ciprian Almonte
Medical College of Pennsylvania
Dept. of Anatomy and Neurobiology E-mail: almonte-at-medcolpa.edu
3200 Henry Ave. Voice: (215) 842-4081
Philadelphia, PA 19129 Fax: (215) 843-9082
__________________________________________________________







From: tivol-at-tethys.ph.albany.edu
Date: Thu, 23 Mar 1995 11:09:55 EST
Subject: Re: Information wanted: Flatbed transparency scanners

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Dear Frank,
There was a used Perkin-Elmer scanner available, which I heard about
from the microscopy list. I don't remember the company name, since we didn't
have the money to get the scanner. They sent me a write-up with specs, but I
don't know whether I still have it. Perhaps you or Nestor can search the ar-
chives for the posting. Good luck.
Yours,
Bill Tivol




From: David Hwang-RA2169 :      david_hwang-ra2169-at-aprdlgtr.sps.mot.com
Date: 23 Mar 95 11:27:24 U
Subject: Position Available for FIB

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Message-Id: {9503231732.AA19857-at-mogate}

REGARDING Position Available for FIB Engineer
Position available for a FIB engineer to join a team working on process
development and failure analysis of ULSI circuits in Motorola, Advanced
Products Research and Development Laboratory, Austin, Texas. The engineer will
be responsible for the setup and operation of a focused-ion-beam facility. The
candidate must have hands-on experience on FIB. Qualified candidates please
send resumes to

ra2169-at-email.mot.com

or mail to

David Hwang
Motorola
APRDL, K-10
3501 Ed Bluestein Boulevard
Austin, TX 78721







From: Lata Prabhu 512-356-7894 :      Lata.Prabhu-at-SEMATECH.Org
Date: Thu, 23 Mar 1995 11:51:00 -0600 (CST)
Subject: FIB TECHNIQUES

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MR-Received: by mta GATEV3; Relayed; Thu, 23 Mar 1995 12:19:38 -0600 (CST)
Alternate-recipient: prohibited
Disclose-recipients: prohibited


Hi,
I am a TEM sample prep technician and is currently preparing
samples by self supportive dimpling, tripod and acid etch(mainly
for planview)techniques. I am interested in new methods out there
being developed and used successfully. Is anyone out there
preparing samples using FIB(Focus Ion Beam) as a tool.We have used
it once in a while to thin our samples prepared with the wedge
technique in the past, but would like to know more.We own a FEI800,
but is currently used extensively for SEMs. Also any tips,and/or
problems to avoid, associated with the sample prep(especially in
the milling dept.) are most welcome. Most of my contamination
problem occurs in the ion-milling process.
Thanks,
Lata Prabhu







From: Lata Prabhu 512-356-7894 :      Lata.Prabhu-at-SEMATECH.Org
Date: Thu, 23 Mar 1995 14:45:00 -0600 (CST)
Subject: returned message

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Message-Id: {9503231940.AA07932-at-riker.ml.wpafb.af.mil}


Just checking to see if the message is getting through, my earlier
one bounced back,
Lata





From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 23 Mar 1995 13:23:56 -0800
Subject: Rotary shadowing of protein

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Message-ID: {n1416157433.59430-at-maillink.berkeley.edu}

Hello all- I am looking for recommendations for evaporation material used in
rotary shadowing of proteins. I am referencing the text "Electron Microscopy
in Molecular Biology", a practical approach; ed. by Sommerville and Scheer.
The method described uses a Balzers electron beam gun to evaporate
tungsten/tantalum. We do not have such a gun but plan to use a standard
thermal evaporation.
Anyone out there have experience/recommendations for evaporation metal,
e.g., platinum, tungsten, palladium, carbon/platinum for finer grains?

Thanks,
Doug Davis
EML Berkeley
(510) 642-2085
doug_davis-at-maillink.berkeley.edu





From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 23 Mar 1995 15:09:49 -0800
Subject: Job Vacancy Listing

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Message-ID: {n1416151173.33639-at-maillink.berkeley.edu}

Subject: Time: 2:52 PM
OFFICE MEMO Job Vacancy Listing Date: 3/23/95

Job opening: Staff Research Associate II
Electron Microscope Laboratory
University of California at Berkeley
$29,200 per annum, 80-100% appointment
Job listing #03-345-30/SL
closing date: 4-14-95
Contact the Office of Employment
Room 7-G
2200 University Ave., Berkeley, CA 94720
(510) 642-1011 general info

Operate and maintain an SEM. Train users in SEM technique, including specimen
preparation. Learn operation of JEOL 9000 freeze-fracture machine and train
and supervise others in its use. Prepare samples for immunoelectron
microscopy (TEM) and occasionally supervise TEM users. Operate cryofixation
equipment for EM sample preparation . Train users in darkrom technique and
supervise use of lab computers. Qualifications: Experience in cryofixation
and immunocytochemical methods. General laboratory skills (preparation of
buffer solutions, operating pH meters etc.). Familiarity with using computer
programs (word processing, spreadsheets). Familiarity with electronic
equipment and its routine maintenance. Darkroom experience. Ability to work
independently.

Personnel will not send out job applications. If the applicants cannot come
to the personnel office to pick up an application they can send a:

1. Cover Letter referencing the job number (03-345-30)
2. Resume

The cover letter and resume should be mailed to:

University of California Berkeley
Berkeley Campus Employment Office
Room 7-G (Ground Floor)
2200 University Ave.
Berkeley, Ca. 94720







From: Lackhlan Ritchie :      etpsemra-at-zonk.geko.com.au
Date: Fri, 24 Mar 1995 13:18:15 +1000 (EST)
Subject: Please UNsubscribe us

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Please UNsubscribe us for this mailing list.
Thank you.




From: IAN HALLETT :      ihallett-at-marccri.marc.cri.nz
Date: Fri, 24 Mar 1995 15:33:33 GMT+1200
Subject: Insect gut fixation

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A message forwarded from my colleague Paul Sutherland

Has anyone got a good fixation method for insect gut tissue which has
a thick peritrophic membrane. At present I am using 2%
paraformaldehyde, 2.5% glutaraldehyde in 0.1M phosphate buffer pH
7.2 and a post fix in 1% osmium tetroxide. With this fix the
microvilli are not well preserved.


Ian Hallett


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-849 3660




From: Mike Gregory :      mgregory-at-pixie.udw.ac.za
Date: Fri, 24 Mar 1995 09:09:21 +0200 (SST)
Subject: ATP-ase for LM and TEM

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We are trying to localise enzyme sites (ATP) on the plasmalemma of salt
glands in halophytic grasses. Has anyone out there got a method(s) for
ATP-ase at the LM level for glut. fixed tissue already embedded in
historesin. Also, methods for ATP-ase in samples for TEM.

Thanks

Yogis






From: Fangl-at-fpms.fpms.ac.be
Date: Fri, 24 Mar 1995 15:18:14 +0100
Subject: help

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Hi, does anyone know FTP site for program of electron diffraction analysis ?
Thanks for your help!
L.FANG




From: tivol-at-tethys.ph.albany.edu
Date: Fri, 24 Mar 1995 12:36:55 EST
Subject: Re: Rotary shadowing of protein

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Dear Doug,
I suggest you look up articles by R.P. Apkarian. He has a Cr coating
method which produces extremely fine grains. He told me of a detailed paper
submitted to Scan. Microsc. Intl. which should have come out in 1994. I have
a few of his papers, but not that one. Good luck.
Yours,
Bill Tivol




From: Dennis Lazof :      dlazof-at-email.unc.edu
Date: Fri, 24 Mar 1995 14:02:20 -0500 (EST)
Subject: Re: Rotary shadowing of protein

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Please UNsubscribe me.

Thanks.




From: samso-at-tethys.ph.albany.edu
Date: Fri, 24 Mar 1995 17:19:03 EST
Subject: unsubscribe

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unsubscribe





From: MED_SCU-at-FRCU.EUN.EG
Date: Sat, 25 Mar 1995 10:35:56 +0000 (O)
Subject:

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First Intarnational Conference on Electron Microscopy
and
Advances in Research in Differcent Fields of Sciance
September 1995
Ismailia - Etap

Sponsored by
Electron Microscopy Center
Suez Canal University
Ismailia - Egypt

Special Topics of the conference:
1- Role of EM in diagnostic virology.
2- Role of EM in diagnosis of tumors cylulogy and urinary atones.
3- Role of EM in ultrastructure pathology of the lung (non neoplastic
conditions).
4- X-ray microanalysis: Applications particularly metalllurgical,
mineralogical, and biological.
5- Scanning EM of plants, animal, ineccts, and mineral material.
6- Study of biological macromolecules from their characteristic
electron diffraction patterns.
7- Skin pathology by EM.
8- Morphological ldentification of antigens by EM.
9- Different low temperature methods for biological EM.
10- Safety measures and maintenance needed for EM.

There will be an equipment exblbition in conjunction with this
meeting. Registration for foreigners will be US $ 150 inclusive of
full board during the time of the meeting.
For further information, contact the organizar:
Prof, Dr. Khalifa Ibrahim Khalifa
Electron Microscvpe Center
Suez Canal University
Ismailia - Egypt
Fax: (20) 64- 329478 ( phone and Fax number)
Fax: (20) 64- 333318 ( Phone and Fax number)

Massage from: sayed Mersal





From: Abdel-Salam Al-Drouby :      WMMAA-at-cardiff.ac.uk
Date: Sat, 25 Mar 1995 14:06:25 GMT
Subject: Is there a list digest?

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Hello,

Could someone please tell me how I can get a digest i.e. daily of the
emails sent on this list, if there is one.

Thank you,

Abdel

Abdel-Salam Al-Drouby
Dept of Medical Microbiology
University of Wales College of Medicine
Heath Park
Cardiff
CF4 4XN
Tel 744724




From: Gary Login :      glogin-at-bih.harvard.edu
Date: Sun, 26 Mar 1995 12:49:40 -0500
Subject: Re: Insect gut fixation using microwave methods

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Microwave irradiation has been shown to enhance the penetration of aldehyde into
insect and plant tissues and to improve fixation of these specimens for LM and
EM. Five references follow:

1. Lindley VA: A new procedure for handling impervious biological specimens.
Microsc Res Tech 21:355, 1992

2. Smid HM, Schooneveld H, Meerloo T: Microwave fixation of water-cooled insect
tissues for immunohistochemistry. Histochem J 22:313, 1990

3. Heumann HG: Microwave-stimulated glutaraldehyde and osmium tetroxide fixation
of plant tissue: ultrastructural preservation in seconds. Histochem 97:341, 1992

4. Login GR, Dvorak AM: Methods of microwave fixation for microscopy. A review
of research and clinical applications: 1970-1992. Prog Histochem Cytochem
27/4:1, 1994

5. Login GR, Dvorak AM: The Microwave Toolbook. A Practical Guide for
Microscopists. Boston, Beth Israel Hospital Press, 1994, 184








In message {7247D4029F-at-marc.cri.nz} "IAN HALLETT" writes:
} A message forwarded from my colleague Paul Sutherland
}
} Has anyone got a good fixation method for insect gut tissue which has
} a thick peritrophic membrane. At present I am using 2%
} paraformaldehyde, 2.5% glutaraldehyde in 0.1M phosphate buffer pH
} 7.2 and a post fix in 1% osmium tetroxide. With this fix the
} microvilli are not well preserved.
}
}
} Ian Hallett


GRL
glogin-at- bih.harvard.edu
Beth Israel Hospital - Department of Pathology
Telephone: 617-667-2034
Fax: 617-667-8676





From: Doug ext. 2470 :      bray-at-hg.uleth.ca
Date: Sun, 26 Mar 1995 19:18:55 MST
Subject: Recording CRT for Hitachi S-500

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Dear Listserver Audience:
The tektronix image recording crt (Current # CRTC818P4S) on our Hitachi S-500
scanning EM is no longer functional. We have temporarily replaced it with a
high resolution monitor, however this is not satisfactory due to the smaller
image. Effort at expanding the image have resulted in some edge distortion and
a loss of resolution.
Does anyone out there have a replacement tube that they wish to sell? If so,
please contact me at:

BRAY-at-HG.ULETH.CA

Thanks,
Doug Bray




From: Doug ext. 2470 :      bray-at-hg.uleth.ca
Date: Sun, 26 Mar 1995 20:14:55 MST
Subject: Recording CRT for Hitachi S-500

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Dear Microscopy Listserver Audience:
The tektronix image recording crt (#CRTC 818 P4S) on our Hitachi S-500 scanning
EM is no longer functional. We have temporarily replaced it with a high res.
monitor, however this is not satisfactory due to problems associated with the
small image produced by the shorter yoke on this crt. Attempts at expanding
the image electronically have resulted in some edge distortion and an unaccept-
able level of resolution loss.
Does anyone out there have a replacement crt of this type? If so, please
E-mail me at:
Bray-at-hg.uleth.ca

Thanks,
Doug Bray




From: kris-at-miat0.vein.hu (Kovacs Kristsf)
Date: Mon, 27 Mar 1995 08:53:22 -0500
Subject: Upgrading old EDS systems

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Dear Friends,

We have an 18 years old EDAX EDS system. The MCA and computer is slow and
unreliable. Does anybody has information on upgrading old EDS systems by
replacing the multichannel analyser and computer with an IBM PC or MAC? We
would like to keep it as cheap as possible.

Thanks!

Kris

Kristof Kovacs
Central Laboratory
University of Veszprem
Veszprem, P.O.Box 158
H-8201 Hungary
Phone: +36-(88)-421684
Fax: +36-(88)-426016
e-mail: kris-at-miat0.vein.hu





From: Keith Moulding :      MCMOULDK-at-usthk.ust.hk
Date: 27 Mar 1995 17:31:16 +0800
Subject: Re: upgrading old EDS systems

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Hello,

Yes, you can upgrade old EDS systems. There is a program called WinEDS.
Coupled with a plug in board for your PC, your detector and pulse
processor you get a Windows based quantitative system.

Sorry I do not have the address of the person who wrote/design the
system, however I will try and track him down (he is in Australia).

This may take a few weeks.


Keith Moulding
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
HKUST
MCPC,
Clear Water Bay,
Kowloon
Hong Kong.

FAX (852) 2-358-2451.




From: waheeschen-at-dow.com (Bill Heeschen 517-636-4005 Materials/ASL)
Date: Mon, 27 Mar 1995 09:21:08 -0500
Subject: Re: Upgrading old EDS systems

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Kris:

I would suggest a call to 4-pi analysis. Their product line emphasizes
upgrading existing EDS systems and works with NIH Image and DTSA (and others)

4-pi analysis
3500 Westgate Dr. Suite 403
Durham, NC 27707-2534
USA


phone:
Mike Sczyz
(919) 489-1757

fax:
(919) 489-1487

electronic mail:
go4pi-at-applelink.apple.com



Bill
==========================
Bill Heeschen/Analyt. Sci.- Mat. Char. -----
1897-F Building / The Dow Chemical Co. --- ---
Midland, MI 48667 U.S.A. --- D O W ---
phone: (517)636-4005 fax: (517)636-5453 --- ---
Email: waheeschen-at-dow.com -----
========================== R




From: jmcgee-at-lunatic.er.usgs.gov (Jim McGee)
Date: Mon, 27 Mar 1995 10:10:20 -0500
Subject: Re: upgrading old EDS systems

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The WinEDS system described by Keith Moulding is designed and marketed by
Paul Thomson:
Thomson Scientific Instruments
216 Drummond Street, Carlton, 3053,
Victoria, Australia
phone (03) 663 2738.
fax (03) 663 3680

I had the opportunity to demo this system at the New Orleans MAS/MSA. It
is an impressive package. This is not an endorsement, just a personal,
scientific opinion.

Jim McGee


U.S. Geological Survey
Reston, VA 22092





From: mullerw-at-rmslab.rockefeller.edu
Date: Mon, 27 Mar 1995 10:23:19 EST
Subject: Sending messages

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Dear Colleagues:

This is a test. I tried to resend a message to this address and it was retu
returned by the Rockefeller mail director. I couldn't resend it. Let's see if
this gets out.

Bill Muller





From: tvoiles-at-unlinfo.unl.edu (Todd Voiles)
Date: Mon, 27 Mar 1995 10:35:44 -0500
Subject: EDS Upgrades

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Message-Id: {199503271600.RAA27751-at-gate.sbbio.be}

Does anybody know how much the WinEds costs?

Seems to be a great disparity between the other packages I've looked at :

DTSA $800 and quirky
Flame $7000 and Fast


Anybody know of any pakages somewhere in the middle?
Center for Materials Research and Analysis
Central Facility for Electron Microscopy
University of Nebraska at Lincoln

Todd Voiles
tvoiles-at-unlinfo.unl.edu





From: BARRY PYLE :      umbbp-at-msu.oscs.montana.edu
Date: Mon, 27 Mar 1995 10:04:30 MST
Subject: Fluorescent in situ hybridization/Bacteria

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I am trying to do fluorescent in situ hybridization with bacteria directly on
Nuclepore polycarbonate membrane filters, and need to omit the alcohol series
that is usually included in these protocols. Does anyone have any F.I.S.H.
protocol that does not include the alcohol series? It doesn't need to be a
membrane filter method. I am, however, interested in any F.I.S.H. membrane
filter protocols. What types of membranes work best?

Thanks, Barry Pyle, Montana State University - Bozeman.




From: RYERSEJS :      RYERSEJS-at-SLUVCA.SLU.EDU
Date: Mon, 27 Mar 1995 14:06:37 -0600 (CST)
Subject: INSECT GUT FIXATION

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I don't know how thick you mean for the peritrophic membrane, but
I've had good results by injecting fixative into the gut lumen and letting
it work awhile before dissecting the gut out in fixative solution. This works well for lep guts. The fixative etc is described in Ryerse et al, Tissue and
Cell, 24:751-771 1992. See discussion of preventing midgut cell surface
blebbing on p 768 by injecting fix directly into the gut lumen. Good luck.
Jan Ryerse, Pathology, St. Louis University Med School, St. Louis, MO





From: Laurie Frederick :      frederick_laurie-at-vanlab.paprican.ca
Date: Mon, 27 Mar 1995 09:38:14 PDT
Subject: Re: Upgrading old EDS systems

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Message-Id: {MAILQUEUE-101.950327093814.384-at-vanlab.paprican.ca}
To: microscopy-at-aaem.amc.anl.gov

Hi,
For an economic EDX system set up to utilize your existing detector, I
suggest you contact the following:

Dapple Systems, Sunnyvale CA , (408) 733-3283
They supply a PC or Mac based system which I believe is passive
only( ie. does not take control of the beam)

4pi Analysis, Inc. Durham NC , (919) 489- 1757
They supply a Mac or Power Mac based system (with beam
control?)

I have only read their brochures not used the systems, but I think they
are worth a look.

Good luck,
Laurie

Kristsf Kovacs wrote:
}
} We have an 18 years old EDAX EDS system. The MCA and computer
is slow and
} unreliable. Does anybody has information on upgrading old EDS
systems by
} replacing the multichannel analyser and computer with an IBM PC or
MAC? We
} would like to keep it as cheap as possible.
}
} Thanks!
}
} Kris
}
} Kristof Kovacs
} Central Laboratory
} University of Veszprem
} Veszprem, P.O.Box 158
} H-8201 Hungary
} Phone: +36-(88)-421684
} Fax: +36-(88)-426016
} e-mail: kris-at-miat0.vein.hu
}
}

______________________________________________________________________
Laurie Frederick, A.SC.T. PAPRICAN
Corrosion Control Group 3800 Wesbrook Mall
The Pulp and Paper Research Vancouver, B.C.
Institute of Canada Canada V6S 2L9

Email: frederick_laurie-at-vanlab.paprican.ca
Tel: 604-222-3200 Fax: 604-222-3207




From: mullerw-at-rmslab.rockefeller.edu
Date: Mon, 27 Mar 1995 11:25:10 EST
Subject: Position available

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Dear Microscopy colleagues:

A fully-funded position is available in my laboratory for a postdoctoral
fellow or an advanced EM technician who can work independently.
I am looking for someone with experience in thin sectioning of mammalian
tissue culture cells and tissues. Specifically, this project involves immunoEM
analysis by post-embedding procedures (e.g. Lowicryl or L.R. White) and/or
ultrathin frozen sectioning.
I am an Associate Professor at The Rockefeller University in New York.
For those of you who may not know, Rockefeller is located in one of the best and
safest areas of New York City. With neighboring Cornell Medical School and
Sloan-Kettering Cancer Center, this is one of the most scientifically exciting
communities to work in. Subsidized housing is available across the street, and
child care is available on campus.
My laboratory is studying endothelial adhesion molecules and their role
in inflammation. We are taking a multidisciplinary approach using techniques
of cell biology, immunology, molecular biology, and biochemistry. We have
identified and cloned two unique adhesion molecules concentrated in the inter-
cellular borders between endothelial cells. These are PECAM-1 (CD31) and the
endothelial-specific cadherin, cad5 (VE-cadherin). Our lab has shown that PECAM
is required for the migration of leukocytes through the endothelial junctions
during inflammation. The project involves determining the ultrastructural
distribution of these proteins along the endothelial junctions in vitro and
in vivo. (We have also cloned the corresponding mouse PECAM and cad5.) We
believe that part of the function of these molecules is determined by their
distribution along the membrane.
This would be an excellent oppportunity for someone trained in electron
microscopy to further his/her skills as well as to learn new techniques of
molecular biology and immunology. Our group interacts well and often, so that
everyone can benefit from the others' knowledge and skills.

Interested parties should send a letter along with a C.V. to the address
below. For specific questions or more details, please fax (212) 327-8875. I
look forward to hearing from you.

Sincerely,

William A. Muller, MD, PhD
Laboratory of Cellular Physiology and Immunology
Box 176
The Rockefeller University
11230 York Avenue
New York, NY 10021-6399


P.S. Thanks to Chris Jeffries and Jeanne Barker for helping me connect to this
listserver.




From: PRING :      richard.pring-at-bbsrc.ac.uk
Date: Tue, 28 Mar 1995 04:19:50 -0600
Subject: embedding plant material

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I have problems in embedding two types of material, one is developing oil seed
rape pods in order to look at changes leading to pod shatter. Even after
several days in 100% Spurr resin, the centre of the section, which often
comtains the developing seed, remains soft and falls out when sectioning.
Similarly, in a study on the infection of rubber leaves by colletotricum spp.,
when sectioning infected material the sections split along the leaf surface.
Any ideas on overcoming these problems? I routinely use Spurr hard resin and go
through propylene oxide after ethanol dehydration. I have also tried holding
the specimens at 45C in the moulds for 24h before raising the temperature to
65C for full polymerisation. I have tried LRWhite with no improvement

Richard pring-at-lars.bbsrc.ac.uk




From: Jan Coetzee EM Univ Pretoria :      JANC-at-ccnet.up.ac.za
Date: Tue, 28 Mar 1995 14:51:03 GMT+2
Subject: Re: embedding plant material

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Richard Pring mentioned:
} I have problems in embedding two types of material, one is developing oil
} seed rape pods in order to look at changes leading to pod shatter. Even
} after several days in 100% Spurr resin, the centre of the section, which
} often comtains the developing seed, remains soft and falls out when
} sectioning. Similarly, in a study on the infection of rubber leaves by
} colletotricum spp., when sectioning infected material the sections split
} along the leaf surface. Any ideas on overcoming these problems? I routinely
} use Spurr hard resin and go through propylene oxide after ethanol
} dehydration. I have also tried holding the specimens at 45C in the moulds
} for 24h before raising the temperature to 65C for full polymerisation. I
} have tried LRWhite with no improvement

Very often the failure to infiltrate material adequately with resin is
due to inadequate fixation and not to inadequate resin infiltration.
Especially when processing oily samples it is necessary to fix {very} well to
destroy all membrane semi-permeability and to stabilise the lipids. Going
to extremes during the fixation may lead to substandard structural
preservation, but may be the only way to adequately infiltrate the sample.
One such overkill schedule is: Fix material 24h at 40deg C in 2.5%
glutaraldehyde, then postfix for another 24h in several changes of
osmium, also at 40deg C (take due care during this step). Dehydrate in
acetone, use a few prop. oxide rinses and embed in your standard resin.
Other methods (adding Malachite Green to aldehydes in DMSO) are also known
and may very well work.
All these methods have in common that they are not optimal as far as
structural preservation is concerned, but sometimes they may be the only ones
that will result in thin sections.
Richard's second problem, that of the adhesion of the resin to the leaf
surface is a result of the wax layer on the leaf surface forming a parting
layer between the leaf and the resin. One solution is to use an epoxy
formulation that is not as easily parted from cuticular waxes - Quetol may be
a possibility.











Jan Coetzee
Unit for Electron Microscopy Tel:+27-12-420-2075
University of Pretoria Fax:+27-12-342-1738
Pretoria Internet:janc-at-ccnet.up.ac.za
0002 South Africa




From: USRND::U096585 U096585 27-MAR-1995 10:15
Date: Tue, 28 Mar 1995 08:20:09 -0500
Subject: Re: Upgrading old EDS systems

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Greetings Kristof...

I have a an old Kevex EDS system hooked up to an "old" Cameca MBX
microprobe....both about 15 years old. I've recently replaced the multi-
channel analyzer with a 4pi processing system and a Mac computer. The 4
pi system costs around $6,000 and is easy to incorporate into many
different EDS systems.

The 4 pi is relatively cheap, it's efficient and easy to operate and is
capable of interfacing with many different EDS systems. Not only can it
generate very nice EDS spectra through DTSA, it does an excellent job
imaging elementally specific X-ray transitions. The quality of these
EDS maps is close to that of the digital WDS maps that we generate on
our SX-50 microprobe. It's really quite impressive.

If you are interested, I would be happy to FAX you some information on
the 4 pi system as well as some EDS images we have generated using that
system. Just let me know....

or here's 4 pi info:

4 Pi Analysis, Inc.
3500 Westgate Dr., Suite 403
Durham, NC 27707-2534
Tel: 919-489-1757
FAX: 919-489-1487

e-mail
go4pi-at-applelink.apple.com

Good Luck and best regards

Cary Black
Microscopist
The Dow Chemical Co.

ckblack-at-dow.com
517-636-5760




From: Giles John E Jr :      giles_john_e_jr-at-space.honeywell.com
Date: 28 Mar 1995 09:05:57 U
Subject: SEM: Thermal Printers

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Has anyone had experience with long term storage of images from a thermal
printer (Codonics, Seikosha, etc.)? I have noticed that the images start to
fade away after exposure (months) to fluorescent lights. I have been told that
this will happen even when they are protected from exposure to light. Any
suggestions for storage or other experience would be appreciated.

John Giles
Honeywell Space Systems
(813) 539-2270
(813) 539-3630 Fax
e-mail: jegiles-at-space.honeywell.com




From: OSRAM Sylvania :      osiit9-at-epix.net
Date: Tue, 28 Mar 1995 18:14:05 +0000 (GMT)
Subject: Re:Thermal print storage problems

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Message-Id: {199503281401.PAA03757-at-gate.sbbio.be}

John,

We've had a Seikosha thermal printer for 4 years and still have some of
the first images printed with it saved. The images have faded slightly
from gray to brown, but you can only tell if you hold next to a new
print. The best storage for them is in a binder or folder that is stored
in a closet or file cabinet. As long as they aren't exposed to drastic
variations in heat they should store for long periods of time with
minimal fading.


Gail Meyers
OSRAM SYLVANIA INC
(717) 268-5340




From: Heinz Hemken :      hhemken-at-cell.cinvestav.mx
Date: Tue, 28 Mar 1995 13:36:38 -0800 (PST)
Subject: Re: Upgrading old EDS systems

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Just out of curiosity, what is an EDS system?

------------------------------------------------------------------
Heinz Hemken
Departamento de Biologia Celular, CINVESTAV-IPN
http://cell.cinvestav.mx/bchh.html





From: gkrichau-at-unlinfo.unl.edu (Gary Krichau)
Date: Tue, 28 Mar 1995 15:19:18 -0500
Subject: Subscribe microscopy

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Subscribe microscopy Gary Krichau

Gary Krichau

Central Facility for Electron Microscopy
University of Nebraska-Lincoln
___________________________________________





From: JOHNA-at-SCI.WFEB.EDU
Date: Tue, 28 Mar 1995 09:49:06 -0400 (EDT)
Subject: PLP fix

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Dear Michel,

PLP fix recipe can be found: Nakane, Paul K., 1975. Recent progress in the
peroxidase-labeled antibody method. Ann. N.Y. Acad. Sci. 254:203-211.

There also is an abstract in the Abstacts of thr 13th Annual Meeting of
ASCB by McLean and Nakane - # 418 on page 209a.

It's a good fixative for the preservation of both antigenicity and
ultrastructure.

Good Luck,

JOHNA

___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Experimental Biology |
| 222 Maple Avenue |
| Shrewsbury, MA 01545-2737 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfeb.edu |
| |
|_________________________________________________|





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 28 Mar 1995 17:03:18 -0600 (CST)
Subject: EDS is.....

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EDS is the abbreviation for

Energy Dispersive Spectroscopy, which is probably more
correctly called X-ray Energy Dispersive Spectroscopy (XEDS)
but various other rearrangements of the letters exist in
the literature (EDXS,...)

It is an analytical methodology in which a solid state semi-conducting
detector (either Si or Ge based) is used to measure the energy distribution
of X-rays emitted from a specimen. The X-ray's may be excited by
some sort of probe (usually a focussed beam of electrons, ions,
or photons). The "Spectrum" is then displayed on a graphical output
device (read computer screen) and the relative intensity of
the measured characteristic X-ray peaks, analyzed to determine
the relative elemental composition of the material.

Any good text book on Scanning Electron Microscopy or Electron
Probe Microanalysis (published within the last 10 years or so
will have a chapter or two on the subject).



Nestor....
Your Friendly Neighborhood SysOp.






From: xxie-at-lsuvm.sncc.lsu.edu (Xiaogang Xie)
Date: Tue, 28 Mar 1995 18:34:22 -0600
Subject: Re: Upgrading old EDS systems

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An article about 4pi system for EDS was published in SCANNING Vol.
14, 233-240 (1992). The title is:

Mac X-ray: The NIST 'DTSA' program and the 4pi analysis board.

one of the authors is:
David C. Joy
EM lab
U. of Tennessee
Knoxville, TN 37996-0810

Our lab also installed both the 4pi spectral engine boards and the
4pi scanning interface box. We have been enjoying NIH Image and several
other programs in playing the SEI, BEI, and X-ray mapping.

Xiaogang

****************************************
* Xiaogang Xie *
* Department of Geology and Geophysics *
* Louisiana State University *
* Baton Rouge, LA 70803 *
* Office (504)388-2240 *
* Fax (504)388-2302 *
****************************************






From: sfzane-at-unccvm.uncc.edu (Sandra F. Zane)
Date: Tue, 28 Mar 1995 11:51:28 -0500
Subject: curing resin summary

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As promised, following is the summary of responses received from my
question, "How many of you have your curing ovens vented to a hood?".

From a total of 11 responses, one containing knowledge of two others,
there were only two who were not curing their resins in, or venting their
ovens to a hood. Both are making provisions to do so. Nine actually place
their ovens in the hood while two are vented to the hood.
There was only one reference sited...that being an article in the
Proceedings of the RMS Vol. 16, pp. 265-270, 1981.
Most of us seem to subscribe to the "just to be sure" school of reason.
I attempted to thank each of you who responded individually, but I
think that I may have lost one of you. So let me say now....thank you very
much.

Sandra Zane
Sandra F. Zane, EM Tech.
Biol. Dept. UNCC
Charlotte, NC 28223
sfzane-at-unccvm.uncc.edu
Fax (704) 547-3128





From: A. Kent Christensen :      akc-at-umich.edu
Date: Tue, 28 Mar 1995 10:19:46 -0500 (EST)
Subject: Re: TEM: PLP fixative reference/recipe

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The original paper on PLP was McLean and Nakane 1974, J Histochem
Cytochem 22:1077-1083. Also see Pino 1984, Stain Technol. 59:307.

Some of the most impressive uses of PLP came out of Marilyn
Farquhar's lab. If I remember correctly the ICC that Farquhar/Brown did
utilized PLP, and would be a good source of method. For example, see:
Cell 36:295(1984). PNAS 81:5135 (1984). JCB 106:1863 (1988). Meth Cell
Biol 31:553 (1989).

Good luck.

A. Kent Christensen
Department of Anatomy and Cell Biology
University of Michigan Medical School
Ann Arbor, MI 48109-0616
{akc-at-umich.edu}

---------------------------------

On Mon, 27 Mar 1995, Michel Deschuyteneer wrote:

} I would greatly appreciate the reference and/or the recipe for the
} Paraformaldehyde - Lysine - Periodate (PLP) fixative.
} Thank you very much in advance.
}
} Regards,
} Michel.
} Michel Deschuyteneer deschuyt-at-sbbio.be
} Scientist - Electron Microscopy Laboratory
} SmithKline Beecham Biologicals
} Rue de l'Institut, 89 - B1330 Rixensart, BELGIUM
} Tel: +32-2-656 9290 Fax: +32-2-6568113
}
}
}
}




From: Chris Varga :      chris-at-lis.rch.unimelb.edu.au
Date: Wed, 29 Mar 1995 13:37:35 +1000
Subject: Re: TEM: PLP fixative reference/recipe

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Please unsubscribe!





From: Dr. Molnar Peter :      MOLNARP-at-lib.dote.hu
Date: Wed, 29 Mar 1995 11:26:06 GMT+0100
Subject: subscription request

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From: Richards, P, Peter :      RETEP-at-anat.uct.ac.za
Date: Wed, 29 Mar 1995 14:09:14 SAST-2
Subject: Re:Diamond Knives

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Message-Id: {m0rttW7-0009hbC-at-uctmail2.uct.ac.za}

To Everyone out there:

Just doing a quick survey as to the price of Diamond microtomy knives.

Could anyone who has bought one recently tell me what the
going cost is for a diamond 5mm ultramicrotyomy knife is, in $ or
pounds sterling?

Thanks in advance

Peter
_______________________________________________________________


-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
-at- -at-
-at- Peter D. G. Richards -at-
-at- Dept Anatomy and Cell Biology -at-
-at- UCT Medical School -at-
-at- Observatory -at-
-at- 7925 -at-
-at- RSA -at-
-at- Tel: 021-406 6285. -at-
-at- Internet: retep-at-anat.uct.ac.za -at-
-at- -at-
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-




From: ckblack-at-dow.com (U096585)
Date: Wed, 29 Mar 1995 08:21:17 -0500
Subject: Re: EDS system upgrades

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Greetings Kristof...

I have a an old Kevex EDS system hooked up to an "old" Cameca MBX
microprobe....both about 15 years old. I've recently replaced the multi-
channel analyzer with a 4pi processing system and a Mac computer. The 4
pi system costs around $6,000 and is easy to incorporate into many
different EDS systems.

The 4 pi is relatively cheap, it's efficient and easy to operate and is
capable of interfacing with many different EDS systems. Not only can it
generate very nice EDS spectra through DTSA, it does an excellent job
imaging elementally specific X-ray transitions. The quality of these
EDS maps is close to that of the digital WDS maps that we generate on
our SX-50 microprobe. It's really quite impressive.

If you are interested, I would be happy to FAX you some information on
the 4 pi system as well as some EDS images we have generated using that
system. Just let me know....

or here's 4 pi info:

4 Pi Analysis, Inc.
3500 Westgate Dr., Suite 403
Durham, NC 27707-2534
Tel: 919-489-1757
FAX: 919-489-1487

e-mail
go4pi-at-applelink.apple.com

Good Luck and best regards

Cary Black
Microscopist
The Dow Chemical Co.

ckblack-at-dow.com
517-636-5760





From: u096585-at-mdanl3.md.dow.com (U096585)
Date: Wed, 29 Mar 1995 08:21:17 -0500
Subject: Re: EDS system upgrades

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Greetings Kristof...

I have a an old Kevex EDS system hooked up to an "old" Cameca MBX
microprobe....both about 15 years old. I've recently replaced the multi-
channel analyzer with a 4pi processing system and a Mac computer. The 4
pi system costs around $6,000 and is easy to incorporate into many
different EDS systems.

The 4 pi is relatively cheap, it's efficient and easy to operate and is
capable of interfacing with many different EDS systems. Not only can it
generate very nice EDS spectra through DTSA, it does an excellent job
imaging elementally specific X-ray transitions. The quality of these
EDS maps is close to that of the digital WDS maps that we generate on
our SX-50 microprobe. It's really quite impressive.

If you are interested, I would be happy to FAX you some information on
the 4 pi system as well as some EDS images we have generated using that
system. Just let me know....

or here's 4 pi info:

4 Pi Analysis, Inc.
3500 Westgate Dr., Suite 403
Durham, NC 27707-2534
Tel: 919-489-1757
FAX: 919-489-1487

e-mail
go4pi-at-applelink.apple.com

Good Luck and best regards

Cary Black
Microscopist
The Dow Chemical Co.

ckblack-at-dow.com
517-636-5760

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From: Leonard Radzilowski :      lrg8037-at-uxa.cso.uiuc.edu
Date: Wed, 29 Mar 1995 09:50:22 -0600 (CST)
Subject: unsubscribe

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From: Heinz Hemken :      hhemken-at-cell.cinvestav.mx
Date: Wed, 29 Mar 1995 09:06:43 -0800 (PST)
Subject: Re: EDS system upgrades

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Thanks to all who have explained to me what EDS is. I got several
enlightening and courteous replies. This is a great list and a great
community!

Keep up the rich info stream!

------------------------------------------------------------------
Heinz Hemken
Departamento de Biologia Celular, CINVESTAV-IPN
http://cell.cinvestav.mx/bchh.html





From: bdgranby-at-student.wisc.edu (ben granby)
Date: Wed, 29 Mar 1995 12:01:02 -0500
Subject: userlist

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Please add me to uour mailing list. Thank You.





From: bdgranby-at-student.wisc.edu (benjamin granby)
Date: Wed, 29 Mar 1995 12:01:16 -0500
Subject: userlist

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Please add me to your mailing list. Thank You.





From: bdgranby-at-student.wisc.edu (Ben Granby)
Date: Wed, 29 Mar 1995 12:02:47 -0500
Subject: Userlist

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From: bdgranby-at-students.wisc.edu (Benjamin Dov Granby)
Date: Wed, 29 Mar 1995 12:09:49 -0500
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From: sackshk-at-ptbma.usbm.gov (Ken Sacks)
Date: Wed, 29 Mar 1995 15:08:55 -0500
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From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Wed, 29 Mar 1995 14:55:06 -0500 (EST)
Subject: SEM Short Courses for Materials Scientist

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This request is for a colleague:

He is using SEM for industrial applications (various analyses of Clay,
paints, paper, etc). They are looking for a good short course on SEM in
materials science. The dates of both the Lehigh and McCrone courses will
not fit into their schedule. I know of a course at Univ Michigan which I
told them about. Are there any others?-
Thanks for any help you can render.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: Lalonde Sylvie :      lalonds-at-ERE.UMontreal.CA
Date: Wed, 29 Mar 1995 11:47:52 -0500 (EST)
Subject: LM.methacrylate resin.removal and UV polymerization

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Hi folks,

I am trying to do in situ hybridization and immunohistochemistry on
glycol methacrylate-embedded wheat pollen. The reason I am using that
resin, despite its uglyness to work with, is that the infiltration of
pollen is very good and the overall morphology seems to be well
preserved. But to gain a better access to the target in the tissue I
would like to remove the resin after sectionning. The resin was
polymerized with benzoyl peroxide at 55C. Is there a way to remove it
without affecting antigenicity of the tissue???

If I cannot remove the glycolmethacrylate, I am planning to use a mixture
of butyl and methyl methacrylate with a polymerization under UV.
According to litterature, I can remove it with acetone. Some people use
benzoin, others use benzoin ethyl ether; what is the difference between
the two? Can I use bezoyl peroxide instead? I know that UV polymerization
can lead to inconsistancy in the polymerization, is the use of a 4W placed
on top of the specimen at a distance of about 10 cm could be good?

Since I do not subscribe to this group could you send me directly any
suggestions you may have.

Thanks in advance

Sylvie Lalonde
lalonds-at-ere.montreal.ca







From: Dwight Beebe :      beebed-at-ERE.UMontreal.CA
Date: Wed, 29 Mar 1995 12:20:44 -0500 (EST)
Subject: LM: fluorescent probes for ER

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Greetings:
I'm interested in using fluorescent probes to clarify the
relationship between certain cellular structures, specifically the ER and
what I believe to be vacuoles. Some of my colleages have suggested that
the vesicles I believe to be vacuolar in origin may be dilated ER. In
looking for likely probes I've found refences in the Molecular Probes
handbook to DiIC (a long-chain carbocyanine) and Rhodamine B as
ER-specific dyes. However, the cited references are based on experiments
on animals. Ideally, I would like to use two probes that will
discriminate between ER and vacuoles. Does anyone have experience with
these dyes or could someone point me towards some references? I'm
working with leaf tissue, specifically cells with the minor veins.
Many thanks in advance.

Dwight Beebe
IRBV, Dept. de sciences biologiques beebed-at-ere.umontreal.ca
Universite de Montreal Voice:514-872-4563
4101, rue Sherbrooke est FAX:514-872-9406
Montreal, PQ H1X 2B2 Canada








From: almonte-at-medcolpa.edu
Date: Wed, 29 Mar 1995 13:11:13 -0400
Subject: LM: fluorescent probes for ER

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Dear friends,
Thanks for your responses. As you all may remember I was having
some crossing over problem between FITC and Rhodamine-that's what I
thought... My problem seems to be autoflorescense, yeah that simple and
basic, I just overlooked it.
I just looked at the neurons with just plain solution, they
flouresce beautifully under the rhodamine filter, and slightly under the
FITC filter and no fluorescence under the Cy5 filter. Also, I had a control
(no antibodies present) which I fixed with 4% paraformaldehyde and used 3%
normal goat serum as blocking reagent. This control flouresce better under
the FITC filter than under the Rhodamine filter, the opposite of the
control without fixative. It does not flouresce under the Cy5 filter.
Also, I did a control with CY5 and Cy3. Again the cells flouresces
under the FITC and rhodamine Filters but not under the CY5 filter.
So my solution seems to stay away from FITC, and rhodamine. Any
suggestion on how I can better solve my autoflourescence problem will be
appreciated. Thanks again for your responses.
Your friend,
Ciprian

__________________________________________________________
Ciprian Almonte
Medical College of Pennsylvania
Dept. of Anatomy and Neurobiology E-mail: almonte-at-medcolpa.edu
3200 Henry Ave. Voice: (215) 842-4081
Philadelphia, PA 19129 Fax: (215) 843-9082
__________________________________________________________







From: Eric Wang :      ewang-at-u.washington.edu
Date: Wed, 29 Mar 1995 17:27:21 -0800 (PST)
Subject: Re: SEM Short Courses for Materials Scientist

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X-Sender: ewang-at-mead2.u.washington.edu


There is a very good SEM short course given by Botany department of
university of washington in Seattle. This course covers various subject
and the scheduel is flexable. The instructor of the course is Barbra Reine.

Tel # for Botany Department: (206) 543-1942




On Wed, 29 Mar 1995, Jay Jerome wrote:

} This request is for a colleague:
}
} He is using SEM for industrial applications (various analyses of Clay,
} paints, paper, etc). They are looking for a good short course on SEM in
} materials science. The dates of both the Lehigh and McCrone courses will
} not fit into their schedule. I know of a course at Univ Michigan which I
} told them about. Are there any others?-
} Thanks for any help you can render.
}
}
} Jay Jerome
} **************************************************************
} * aka: W. Gray Jerome *
} * Dept. of Pathology *
} * Bowman Gray School of Medicine of Wake Forest University *
} * Medical Center Blvd *
} * Winston-Salem, NC 27157-1092 *
} * 910-716-4972 *
} * jjerome-at-isnet.is.wfu.edu *
} **************************************************************
}
}




From: Kittel Agnes :      kittel-at-kokiux.koki.hu
Date: Thu, 30 Mar 1995 09:30:06 +0200 (MET DST)
Subject: subscription reguest and a guestion

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If someone knows, please, write me, which type of NCAM antibody can work
for post- embedding method? (Araldite 6005 or Epon resin is used and pre
embedding I have made an enzyme histochemical reaction. Fixation :
paraformaldehyde- glutaraldehyde in cacodylate buffer)
Thanks!
Agnes Kittel






From: H2993Pec-at-huella.bitnet
Date: 30 Mar 95 09:56:46 +0100
Subject: Subscription request

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Subscription request





From: Tina Carvalho :      tina-at-halia.pbrc.Hawaii.Edu
Date: Wed, 29 Mar 1995 22:27:56 -1000 (HST)
Subject: C evaporation for EDS

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Both my lab and another lab here in Hawaii have Denton DV-502 vacuum
evaporators. My evaporator is perfectly fine for my needs; I can
carbon coat Formvar grids or prepare pure carbon substrates for TEM.
The other lab, however, wants to carbon coat for SEM, with and without
EDS. Their problem is that they cannot get the carbon to evaorate long
enough to get a thick enough coating, and neither can I on my
instrument. We both have the same spring-steel (?) driven carbon rod
holders (which differ from the gravity-fed system in the very old Denton
in still another Hawaii lab and also from the coiled spring feed on the
non-adjustable holders). The problem seems to be that there is
enough resistance somewhere that everything heats up like crazy, the
moveable parts expand enough to get stuck, and the whole feed process
stops until the unit cools down or blows a fuse, whichever comes first.
This has happened with old holders, brand-new ones, and everything else
I have been able to devise. Has anyone else had and oversome this
problem? Any hints and tips will be appreciated!

Mahalo and aloha,
Tina Weatherby Carvalho
Biological EM Facility
University of Hawaii





From: ddd-at-techunix.technion.ac.il (dd)
Date: Thu, 30 Mar 1995 12:53:55 +0200
Subject: Light scrambler for HBO lamp

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We need information on where to get a light scrambler for homogeneous
illumination -to couple an HBO light source to a Zeiss microscope for
fluorescence work. Sources, FAX numbers, price ranges -any information
would be highly appreciated.
dd




Daniel Dagan,
Dept. Biophysics and Physiology,
Faculty of Medicine, Technion,
Israel Institute of Technology,
POBox 9697
Haifa 31096
ISRAEL

Tel: 972-4-295566
Fax: 972-4-533183
e-mail: ddd-at-techunix.technion.ac.il






From: A. Kent Christensen :      akc-at-umich.edu
Date: Thu, 30 Mar 1995 08:05:54 -0500 (EST)
Subject: Autofluorescence problems

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From your description, it sounds like you need quenching rather than
more blocking. Blocking uses miscellaneous protein to block non-specific
protein-binding sites in the tissue, but doesn't actually deal with
autofluorescence. Quenching uses reducing agents such as ammonium
chloride or sodium borohydride to reduce double bonds in the tissue, which
are usually the main source of autofluorescence. Try the following: make
up a fresh 1% solution of sodium borohydride in distilled water (careful,
borohydride is toxic), and leave a few drops of it on your sections for
about 10 minutes early in your immunocytochemical run (before the primary
antibody step). This should reduce the inherent autofluorescence in your
tissue.

A. Kent Christensen, Department of Anatomy and
Cell Biology, University of Michigan Medical School, {akc-at-umich.edu}

------------------------------------

On Wed, 29 Mar 1995 almonte-at-medcolpa.edu wrote:

} Dear friends,
} Thanks for your responses. As you all may remember I was having
} some crossing over problem between FITC and Rhodamine-that's what I
} thought... My problem seems to be autoflorescense, yeah that simple and
} basic, I just overlooked it.
} I just looked at the neurons with just plain solution, they
} flouresce beautifully under the rhodamine filter, and slightly under the
} FITC filter and no fluorescence under the Cy5 filter. Also, I had a control
} (no antibodies present) which I fixed with 4% paraformaldehyde and used 3%
} normal goat serum as blocking reagent. This control flouresce better under
} the FITC filter than under the Rhodamine filter, the opposite of the
} control without fixative. It does not flouresce under the Cy5 filter.
} Also, I did a control with CY5 and Cy3. Again the cells flouresces
} under the FITC and rhodamine Filters but not under the CY5 filter.
} So my solution seems to stay away from FITC, and rhodamine. Any
} suggestion on how I can better solve my autoflourescence problem will be
} appreciated. Thanks again for your responses.
} Your friend,
} Ciprian
}
} __________________________________________________________
} Ciprian Almonte
} Medical College of Pennsylvania
} Dept. of Anatomy and Neurobiology E-mail: almonte-at-medcolpa.edu
} 3200 Henry Ave. Voice: (215) 842-4081
} Philadelphia, PA 19129 Fax: (215) 843-9082
} __________________________________________________________




From: Richard Lee :      richard_lee-at-qmgate.anl.gov
Date: 30 Mar 1995 11:38:54 -0600
Subject: Formation of geodes

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11:35 AM 3/30/95

Does anyone know the mechanism of geode crystal formation in the midwest? And
why do some geodes contain oil?

I have an Idea!




From: BILL RHOTEN :      RHOTEN-at-musom01.mu.wvnet.edu
Date: Thu, 30 Mar 1995 13:31:05 +1100
Subject: test mess, as in test message

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From: xxie-at-lsuvm.sncc.lsu.edu (Xiaogang Xie)
Date: Thu, 30 Mar 1995 12:48:35 -0600
Subject: an introductary paper about 4pi system

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A message about an article regarding the 4pi system sent out couple of
days ago was bounced back. The paper was published in SCANNING Vol. 14,
233-240 (1992). The title is:

Mac X-ray: The NIST 'DTSA' program and the 4pi analysis board.

One of the authors is:
David C. Joy
EM lab
U. of Tennessee
Knoxville, TN 37996-0810

Our lab has installed both the 4pi spectral engine boards and the
4pi scanning interface unit. We have also been enjoying NIH Image and
several other programs in playing the SEI, BEI, and X-ray mapping.

Xiaogang

****************************************
* Xiaogang Xie *
* Department of Geology and Geophysics *
* Louisiana State University *
* Baton Rouge, LA 70803 *
* Office (504)388-2240 *
* Fax (504)388-2302 *
****************************************






From: Self :      MUSOM01/RHOTEN
Date: Thu, 30 Mar 1995 13:31:05
Subject: test mess, as in test message

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------- Forwarded Message Follows -------

How was the test mess?Microscopy BBS




From: Dennis Shubitowski :      dennis%odin-at-odin.morph.med.umich.edu
Date: Thu, 30 Mar 1995 14:51:44 -0500 (EST)
Subject: Re: Formation of geodes

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Errors-To: {dennis-at-odin.morph.med.umich.edu}



On 30 Mar 1995, Richard Lee wrote:

} Does anyone know the mechanism of geode crystal formation in the midwest? And
} why do some geodes contain oil?

According our resident geologist, geodes form when percolating waters
precipitate into hollow cavities. Whatever happens to be carried with the
water is deposited in layers into the cavity (this includes oil).


Dennis








From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Thu, 30 Mar 1995 14:51:31 -0600 (CST)
Subject: liposomes

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Does anyone have a protocol for visualization of liposomes?
I would like to be able to thin section for TEM. Should anything be seen
at the LM level?

thanks,
Joyce Craig




From: xxie-at-lsuvm.sncc.lsu.edu (Xiaogang Xie)
Date: Thu, 30 Mar 1995 12:06:43 -0600
Subject: advises about filament needed

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Advises about W filament needed:

1. Has anyone made comparison about the performance, life time, and
potential contamination between new filament and rebuilt one. The prices
are four times difference.
2. Which company supplies better batch of filaments. This information may
mail to me directly if you feel it may offend some one.
3. Last year, we had a batch of new filaments that tend to form thin film
of metal sticking on the tip of the filament, though the heating
temperature was low (with slightly large distance between filament and the
cap, and the heating current is limited by a stopper). Is this a problem
due to impurity of filament? Any idea?

Thanks for any input.

Xiaogang

****************************************
* Xiaogang Xie *
* Department of Geology and Geophysics *
* Louisiana State University *
* Baton Rouge, LA 70803 *
* Office (504)388-2240 *
* Fax (504)388-2302 *
****************************************






From: xxie-at-lsuvm.sncc.lsu.edu (Xiaogang Xie)
Date: Thu, 30 Mar 1995 12:44:57 -0600
Subject: Re: C evaporation for EDS

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Tina:
In our lab, the DV-502 is used for C coating SEM and Microprobe
samples for EDS and WDS works.

1. The length and size of the reduced C rod are important factors for the
coating thickness. The coarser C rod does not only supply more C but also
support longer reduced C rod. However, coarser C rod needs higher current
to reach the evaporation temperature, hence it might hit some limits of the
machine, like burn the fuses. This has to be balanced.
2. You also need to properly control the heating current during
evaporating. Specially, you need quickly bring the current back to some
position where evaporation can last longer at the very beginning when the
evaporating starts.

Xiaogang

****************************************
* Xiaogang Xie *
* Department of Geology and Geophysics *
* Louisiana State University *
* Baton Rouge, LA 70803 *
* Office (504)388-2240 *
* Fax (504)388-2302 *
****************************************






From: kayton-at-ohsu.edu (Robert Kayton,MAC,CROET)
Date: Thu Mar 30 17:12:16 PST 1995
Subject: TEM: Gold particle counting

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Message-Id: {m0ruVG4-0007AdC-at-stjohns.ohsu.edu}
Message-Version: 2
} To: microscopy-at-aaem.amc.anl.gov

I have colleague that needs help setting up an automatic computer based method
to count gold particles on TEM micrographs. He has NIH image, a good
Macintosh and can import digital images but is having difficulties getting
accurate counts of the gold particles. If anyone has experience with this
please let me know.

Bob Kayton
kayton-at-ohsu.edu




From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 30 Mar 1995 16:28:16 -0800
Subject: Re: C evap for EDS

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Message-ID: {n1415541637.55689-at-maillink.berkeley.edu}
"Tina Carvalho" {tina-at-ahi.pbrc.hawaii.edu}
X-Mailer: Mail*Link SMTP-QM 3.0.2

Subject: Time: 4:13 PM
OFFICE MEMO RE} C evap for EDS Date: 3/30/95

Tina- check your ceramic insulators through which the rod holder slides.
If the ceramic gets enough carbon or metal on it, it creates a conductive
pathway which corrupts your normal electrical flow and sends current through
the metal parts of the electrode instead of the carbon rod. Could explain the
hot metal and blown fuses from over-amping the system. This can even melt
some of the smaller metal parts.
Cleaning the ceramic is tough because of the porous surface and chances are
the ceramic will crumble from the excessive heating when you dissassble the
holder. Suggest you order some new ones from Denton and keep some spares.
Have you tried the carbon yarn yet? Works well, never sparks or arcs,
heavier thicknesses can be produced by reducing the source to target distance.
Last time I checked, Denton had the best price. -Doug





From: VanderWood-at-aol.com
Date: Fri, 31 Mar 1995 01:37:10 -0500
Subject: Re:C evap for EDS

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We have done a comparison study of rods from different sources, and found
some to be unworkable for SEM/EDS coating in our 502. Rather than belittle
the ones that don't work, I can recomment those from Ladd. Hope this helps.

Tim VanderWood
MVA, Inc.




From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 30 Mar 1995 09:49:11 -0800
Subject: Denton evap

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Message-ID: {n1415565559.16197-at-maillink.berkeley.edu}
"Tina Carvalho" {tina-at-ahi.pbrc.hawaii.edu}
X-Mailer: Mail*Link SMTP-QM 3.0.2

Subject: Time: 9:28 AM
OFFICE MEMO Denton evap Date: 3/30/95

Tina- We have a 502A with turbo. One thing to check immediatly is the
ceramic insulators which the electrode holder slides through. In the past,
repeated applications of heavy coatings will eventually create a conductive
surface coating on these parts and current through the carbon rod will be
reduced as it flows along this alternate pathway. In extreme cases, carbon
will not evaporate and metal parts of the electrode will actually begin to
melt. Good thing your fuses are blowing rather than your top. The insulators
are tough to clean because of the porous surface and you may find that they
crumble to dust when you remove them due to the heating they have been
subjected to. It's good to have a few extras around as this is a chronic
problem.
Have you tried the carbon yarn yet in the Denton? It is impossible to
blowout/arc as with the rods and is much more controllable, just evaporate
until it burns through. Heavier coatings can be acquired by reducing the
distance from the source. Look out for metal contamination of the yarn if you
evaporate shadowing metals; it shows up as tiny electron-dense spots on you
substrates. Use the shutter to protect the unused yarn on the bobbin. You
can get the yarn directly from Denton for the best price; e-mail me if you
need info. -Doug





From: Laurie Frederick :      frederick_laurie-at-vanlab.paprican.ca
Date: Thu, 30 Mar 1995 10:44:55 PDT
Subject: SEM: Carbon Evaporators

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Message-Id: {MAILQUEUE-101.950330104455.320-at-vanlab.paprican.ca}
To: microscopy-at-aaem.amc.anl.gov

Hello,
We are intending to purchase a carbon evaporation unit for coating
SEM/EDX samples . We have received quotes for 6 systems but have
narrowed the choises to the following on the basis of cost:
Denton 502A
Ladd
Edwards 306
The price range for these units is $24,000-$35,000 in Canadian dollars.
We are very tight for money (of course) but do not want to buy a unit
which will give us continuous headaches with overheating, jamming of
the carbon rod feed mechanism or having to evacuate several times in
order to recoat because enough carbon could not be applied the first
time.
If you have experience with any of these units, either positive or
negative, I would appreciate hearing your comments. If you have any
experience with using 1/4" rods vs. 1/8" rods or "reduced section"
versus conically tipped rods, please pass it along.

I was all set to make a purchase before I read the recent comments on
the listserver.
Thanks very much for any assistance.
Laurie


______________________________________________________________________
Laurie Frederick, A.SC.T. PAPRICAN
Corrosion Control Group 3800 Wesbrook Mall
The Pulp and Paper Research Vancouver, B.C.
Institute of Canada Canada V6S 2L9

Email: frederick_laurie-at-vanlab.paprican.ca
Tel: 604-222-3200 Fax: 604-222-3207




From: Laurie Frederick :      frederick_laurie-at-vanlab.paprican.ca
Date: Thu, 30 Mar 1995 14:35:52 PDT
Subject: EDX Contacts

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Message-Id: {MAILQUEUE-101.950330143551.320-at-vanlab.paprican.ca}
To: microscopy-at-aaem.amc.anl.gov

Hi,
I am trying to wrap up a summary of referances on EDX and require
some information:
1) Has the book from MAS entitled "X-ray Spectrometry in Electron
Beam Instruments" by Dave Williams and Dale Newbury actually been
published yet and if so, is there a phone number for orders?

2) Is anyone familiar with an EDX upgrade system from "Aptec"? I
believe it is located somewhere in Ontario, Canada but I am not sure if
the name I have is a company or product name. Contact # ?

3) When and where is the next International Congress on Electron
Microscopy (ICEM) and what is a contact number for information?

4) When and where is the next International Conference on X-ray
Optics and Microanalysis (ICXOM) and what is a contact # for info?

I will be making a summary of the information I have gathered on EDX
available shortly. Thanks for any assistance.
Laurie

______________________________________________________________________
Laurie Frederick, A.SC.T. PAPRICAN
Corrosion Control Group 3800 Wesbrook Mall
The Pulp and Paper Research Vancouver, B.C.
Institute of Canada Canada V6S 2L9

Email: frederick_laurie-at-vanlab.paprican.ca
Tel: 604-222-3200 Fax: 604-222-3207




From: Robert Kayton,MAC,CROET :      kayton-at-ohsu.edu
Date: Fri, 31 Mar 1995 07:04:20 -0800
Subject: TEM: Gold particle counting

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Reply to: RE} TEM: Gold particle counting
Hi Bob,
Your colleague may be suffering from low contrast images. Try acquiring or
scanning in the images with greater contrast range. The contrast of the images
should be such that the black gold particles should be black (an extreme gray
level) and everything else in the image should be a different gray value
(mid-gray to white). For the images that are already digitized try doing a
"Sharpen" or an "Unsharp Masking" operation on the image.

Sincerely,

George McNamara
Universal Imaging Corporation
--------------------------------------

I have colleague that needs help setting up an automatic computer based method
to count gold particles on TEM micrographs. He has NIH image, a good
Macintosh and can import digital images but is having difficulties getting
accurate counts of the gold particles. If anyone has experience with this
please let me know.

Bob Kayton
kayton-at-ohsu.edu







From: Jose.Feijo-at-bio.fc.ul.pt (Jose Feijo)
Date: Fri, 31 Mar 1995 16:44:18 +0000
Subject: Confocal Red line/immuno-labeling in plant tissue

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Message-Id: {9503311445.AA01865-at-dnagel.bio.fc.ul.pt}
X-Sender: bjfeijo-at-skull.cc.fc.ul.pt
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I there:
One of our users has plans to look at entire styles and to do some immuno
labeling of the stylar channel proteins. He wants to get rid of embedding
and/or freeze-sectioning so he figured confocal would be the answer. Among
others, I antecipate some problem with self fluorescence coming either from
chlorophyls and/or lignins. One way around these would be to use the red
line of the Kr-Ar (we have a MRC-600). So, does anyone has experience on
working with secondary antibodies (in this case against chicken) labeled
with red excitable fluorophores in plant tissues? What may the major
drawbacks in this kind of preparation?
Any help would be highly appreciated.


___________________________________________________________
Jose A. Feijo
Dept. Biologia Vegetal, Fac. Ciencias Lisboa
Ed. C2, Campo Grande, P-1700 LISBOA, PORTUGAL

t. + 351.1.7500069 fax + 351.1.7597716
e.mail Jose.Feijo-at-bio.fc.ul.pt
URL: http://www.fc.ul.pt/departs/biologia_vegetal/ejf.html
___________________________________________________________





From: Brian J. Zande :      bz0c+-at-andrew.cmu.edu
Date: Fri, 31 Mar 1995 11:04:56 -0500 (EST)
Subject: New home for SEM

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Message-ID: {IjT2UcW00iV_A3idsG-at-andrew.cmu.edu}

The Carnegie Mellon Research Institute (a division of Carnegie Mellon
University) is trying to find a new home for a 13 year old ISI SS-40
SEM. If anyone out there is interested please send mail to me at
bz0c+-at-andrew.cmu.edu

Thanks
Brian




From: Beth Trend :      trend-at-cems.umn.edu
Date: Thu, 30 Mar 1995 15:06:07 -0600
Subject: TEM: Cryo tem of colloids

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} From the Characterization Facility at the University of Minnesota

MASTER CLASS

Cryo-TEM of Colloidal Materials

=A5 Specimen Preparation

=A5 Cryo-Transfer to the Electron Microscope

=A5 Low-Electron Dose Image and Diffraction Pattern Recording on Film

=A5 Image-Processing and Analysis of Digital Cryo-TEM Images


May 18-20, 1995

Intended Audience

This Master Class is intended for any materials scientists or process eng=
ineers =

working with biological or colloidal materials. This class will explore t=
he use =

of the technique of cryo-transmission electron microscopy to probe the =

microstructure of these samples in the vitrified, hydrated state at resol=
utions =

around 1 nm. Previous TEM experience, although welcome, is not required.=



Description

The lectures will introduce the basics of cryo-transmission electron micr=
oscopy,
including sample preparation and microscopy techniques. Applications to s=
pecific
biological and colloidal materials=D5 samples will be demonstrated, and =

complementary characterization techniques will be described.

The details of sample preparation, focussing on vitrification parameters =
and =

transfer to the microscope, will be described and demonstrated. Imaging =

techniques and interpretation, artifact identification, and quantitative =

analysis will also be covered.

Supervised hands-on laboratories demonstrating the techniques that were =

introduced in the lectures will be interspersed throughout the class. Po=
ssible =

topics include: low dose imaging of soft latex particles; low dose imagi=
ng of =

phospholipid vesicles; image processing of cryo-TEM images using NIH Imag=
e and =

Digital Micrograph; CEVS sample preparation; and video-enhanced light mic=
roscopy
of colloidal materials.

Upon completion of the class, participants will be able to select an appr=
opriate
cryogen, prepare vitrified thin films of colloidal specimens, transfer th=
ese =

specimens to the microscope, and record images free from artifacts caused=
by =

electron beam damage.

Registration

For more information, reply to Beth Trend, trend-at-cems.umn.edu


Instructors

Frank Booy is a Senior Staff Fellow, National Institute of Arthritis =

Musculoskeletal and Skin Diseases at the National Institutes of Health. =
As a =

special expert in electron microscopy, he has worked in the field of =

cryo-electron microscopy of biological materials since 1978, at the CNRS =
in =

Grenoble, France, the European Molecular Biological Laboratory in Heidelb=
erg, =

Germany, and the Rijksuniversiteit Groningen in the Netherlands. Dr. Boo=
y is =

particularly involved in the use of cryo-electron microscopy and 3-D imag=
e =

reconstruction techniques to localize the proteins that make up the =

nucleocapsids of herpes simplex virus.


John Minter, a Research Associate in the Analytical Technology Division o=
f the =

Eastman Kodak Company, is the technical group leader of the Quantitative =
Colloid
Microscopy Group. Minter=D5s group applies cryo-TEM and electron crystall=
ography =

to the study of photographic colloids. During 1990, he was an Industrial =
Fellow =

at CIE collaborating with Professors H. Ted Davis and L. E. Scriven to st=
udy =

surfactant morphology and crystal precipitation by cryo-TEM. He is curren=
tly an =

Adjunct Professor at CIE and Industrial Co-chair of the CIE Characterizat=
ion =

Facility Advisory Committee.

David P. Siegel is a Senior Scientist in the Research & Development Dept.=
, =

Procter & Gamble Co. His general interest areas are membrane biophysics =
and the
physical chemistry of biologically relevant lipids. Dr. Siegel is partic=
ularly =

interested in the mechanisms of biomembrane fusion and of lamellar-to-inv=
erted =

phase transitions in phospholipids. Since 1991, he has used cryo-TEM and=
=

time-resolved cryo-TEM to image intermediates in these processes.

Schedule

Thursday May 18 =

8:00 a.m. Registration =


8:30 Lecture I: Introduction to Cryo-TEM
=A5 Basics steps in cryo-TEM
=A5 Biological and colloidal problems that can be solved using c=
ryo-TEM =

=

10:15 Lecture II: Sample Preparation, Vitrification, Storage, and Trans=
fer to =

the Microscope
=

Lunch =


1:00 p.m. Lab Session - Shepherd Labs

Dinner

7:00-10:00 Lab Session continues as needed
=


Friday, May 19

8:00 Coffee =


8:30 Lecture III: Imaging in Cryo-TEM
=A5 Operation of Electron Microscope
=A5 Recognizing the good samples to examine
=

10:15 Lecture IV: Image interpretation, processing, and analysis
=A5 Artifacts - a rogue's gallery
=A5 Quantitative analysis

11:45 Discussion Session

Lunch

1:00- 6:00 p.m. Lab Session - Shepherd Labs


Saturday, May 20

9:00 a.m.-1:00 p.m. Optional Laboratory Session - Students' samples





____________________________________________________________________=
___
Beth Trend trend-at-cems.umn.edu (faster) or btrend-at-maroon.tc.=
umn.edu
Coordinator, Characterization Facility University of Minnesota
Center for Interfacial Engineering =

100 Union St SE Room 187 phone 612-624-1365 fax 612-626-7530=

Minneapolis, MN 55455 =






From: Brian J. Zande :      bz0c+-at-andrew.cmu.edu
Date: Fri, 31 Mar 1995 12:08:00 -0500 (EST)
Subject: new home for ISI

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-ID: {cjT3Pka00iVG44Uk17-at-andrew.cmu.edu}

The Carnegie Mellon Research Institute (a division of Carnegie Mellon
University) is trying to find a new home for a 13 year old ISI SS-40
SEM. If anyone out there is interested please respond to me at
bz0c+-at-andrew .cmu.edu

Thanks,
Brian




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