} CAN ANYONE RECOMMEND AN INEXPENSIVE FRAME GRABBER THAT CAN EASILY INTERFACE } WITH AN ANALOG JEOL 840-I , PC PREFERABLE . I HAVE A 486 WITH 16 MEG RAM } THATS READY FOR IT BUT COULD ALSO GET HOLD OF A MAC. I ALSO HAVE ALL THE } IMAGE ANALYSIS SOFTWARE. MY ONLY PRESENT WAY TO DIGITIZE SEM IS BY SCANNING } MY 4X5 NEGS, BUT WOULD LIKE TO SKIP THE PHOTO STEPS. ATTEMPTING TO QUANTITATE } IMMUNO GOLD SIGNALS IN BSE MODE (SLOW SCAN)
It is difficult to interface signals from the 840, but a board that does this successfully is the ImageSlave from MEECO. This works with a PC and is not too difficult to install if you take great care to use correctly routed, shielded cable. The board will grab 840 signals at Slow Scan 1, Slow Scan 2 and Photo Scan. TV- rate and Ultra-rapid scan modes are not supported. Contact: Steve Wisbey 10 Seville St North Parramatta N.S.W. 2151 Australia Tel 02-630-7755 Fax 02-630-7365
I do not have any commercial interest in this company, other than as a customer.
Jan Coetzee Unit for Electron Microscopy Tel:+27-12-420-2075 University of Pretoria Fax:+27-12-342-1738 Pretoria Internet:janc-at-ccnet.up.ac.za 0002 South Africa
There are two solutions to your problem. You can purchase a gravity feed dewar with a low-warning switch and automatic fill (10L, 20L, 30L, 50L), or you can purchase an automatic liquid level control with a solenoid as you remember to attach to an existing dewar. Please contact me directly by fax (413-789-2786) or phone (800-992-9037) for pricing and other commercial details. Steven Slap, Vice-President, Energy Beam Sciences
I'm interested in commercially available liquid nitrogen level auto filling devices-i.e.some kind of solenoid valve arrangement that keeps the dewars filled, automatically. I seem to recall seeing these on EDS detector dewars, while I was in Berkeley, but I haven't run across anything amongst the retailers.
} Hi everyone - } } I'm looking for a source of the plastic sheet material made of ACLAR } (TM?). A source of slides and/or coverslips made of the stuff would be } ideal, but I will go for anything that might be used in lieu thereof. } Also, if anyone has experience using this material for TEM embedding, I } would be interested in their technique and opinion. Thanks in advance for } any help you can give me. } } Dan }
} %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% } % % } % Daniel Possin Work: 206/ 543-7489 % } % Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 % } % University of Washington Home: 206/ 778-1714 % } % Seattle WA 98195 USA Email: oemlab-at-u.washington.edu % } % % } % "The chinese expression 'cheung meng ba sui, gong hey fat choy' is % } % equilvalent to Vulcan expression 'live long and prosper'. It's a % } % small universe and getting smaller everyday". % } % % } %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% } -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- Dear Dan,
Aclar plastic is available from ProPlastics, Inc., P.O. Box 1489, Linden N.J. 07036
Phone: 908-925-5555. This info is now 3 years old, which is when I made the minimum purchase which will last several lifetimes,/. I am willing to send a small sample to anyone who would like to try it before they buy.
The material is optically clear and will not bind to any embedding resin we have tried. Many cell lines will grow on it. It is flexible so it tends to curve if steps are not taken to prevent that. I have taken to "spot welding" it with a hot dissecting needle to a piece of an old EM negative or to the bottom of a culture dish.}
Let me know if you would like some to try. -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- -at- Greg Erdos Phone: 904-392-1295 -at- -at- Scientific Director, -at- -at- ICBR Electron Microscopy Core Lab -at- -at- 218 Carr Hall Fax 904-846-0251 -at- -at- University of Florida E-mail: gwe-at-biotech.ufl.edu -at- -at- Gainesville, FL 32611 -at- -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Patty Jansma Tel:602-621-6671 plj-at-manduca.neurobio.arizona.edu Arizona Research Labs Division of Neurobiology University of Arizona
On Mon, 31 Jul 1995, Daniel Possin wrote:
} Hi everyone - } } I'm looking for a source of the plastic sheet material made of ACLAR } (TM?). A source of slides and/or coverslips made of the stuff would be } ideal, but I will go for anything that might be used in lieu thereof. } Also, if anyone has experience using this material for TEM embedding, I } would be interested in their technique and opinion. Thanks in advance for } any help you can give me. } } Dan } } %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% } % % } % Daniel Possin Work: 206/ 543-7489 % } % Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 % } % University of Washington Home: 206/ 778-1714 % } % Seattle WA 98195 USA Email: oemlab-at-u.washington.edu % } % % } % "The chinese expression 'cheung meng ba sui, gong hey fat choy' is % } % equilvalent to Vulcan expression 'live long and prosper'. It's a % } % small universe and getting smaller everyday". % } % % } %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% }
I have some live transparent tissue and would like to see the membranes in bright field. For practicle reasons I can't use phase contrast,DIC etc. to see them. Can anyone suggest a dye/stain that won't immediately kill the cells. Ideally the cells should remain active for an hour or two after dye application and I'm not worried about longer term cell viability.
Again, for practicle reasons the dye sould NOT be fluorescently based as I have tried several of these without success due to practicle considerations.
TerryMicroscopy Unit School Of Biological Sciences The University Of Auckland Level 1, Thomas Building Private Bag 92019 Auckland, NEW ZEALAND
You could try the ImageSlave board. It captures the images at 1K x 1K when you hit the photo button. Images are saved as TIFF files and it has the ability to increment the file number series so you do not have to type in a filename each time. Currently runs under DOS, but a Windows version will be very soon.
Your PC sounds fine for it. We have two of the boards here and are very happy with them, they have saved a lot of Polaroid film.
It depends where you are, but there is an advert in Microscopy and Analysis for the board. Otherwise contact Steve Wisbey, Meeco Holdings PTY. Ltd, FAX +61 (2) 6307365 (Australia).
Keith.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr. Keith Moulding, ~ Materials Characterisation and Preparation Centre, ~ Hong Kong University of Science and Technology, ~ Clear Water Bay, ~ Kowloon, ~ Hong Kong. ~ ~ Tel: (852) 2358 8724 ~ Fax: (852) 2358 2451 ~ ~ E-mail: mcmouldk-at-usthk.ust.hk ~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dear All, Does anyone know the E-mail of the secretariat of the ICXOM - The 14th International Congress on X-ray Optics and Microanalysis? ICXOM will be held on Aug.29 - Sept.2, 1995 in the Guangzhou, China. I very need the fast communication with the Organizing Commitee and the post is too slow. Many thanks!
Sergey Kramar CFPM, Moscow Institute of Electronic Technology, Moscow 103498, Russia E-mail: lemi-at-mx.iki.rssi.ru (to S.Kramar)
} CAN ANYONE RECOMMEND AN INEXPENSIVE FRAME GRABBER THAT CAN EASILY INTERFACE } WITH AN ANALOG JEOL 840-I , PC PREFERABLE . I HAVE A 486 WITH 16 MEG RAM } THATS READY FOR IT BUT COULD ALSO GET HOLD OF A MAC. I ALSO HAVE ALL THE } IMAGE ANALYSIS SOFTWARE.
One commersially available solution could be the JEOL SemAfore, which is a= =20 passive listening digitizer board for IBM compatible PC operating under MS= =20 Windows. This will digitize the slow scan signal from a JEOL JSM-840 (and=20 most other JEOL SEMs) with the same pixel resolution (typically 1900x1400=20 pixels) as displayed on your photo CRT.
The digitized image is stored in standard BMP format, which is read by most= =20 other MS Windows applications. If you still wish to record the original or= =20 modified image photographically, you can send the image back to the photo=20 CRT of your SEM.
The minimum PC configuration needed is a 486SX with 8 MB RAM and MS-Windows= 3.1.
As I don=B4t know where you are located, please contact me directly for=20 commercial details.
In a recent wirlwind clearing of stored E-mail messages I have lost the information regarding a Vacuum Technology Book which has been mentioned on the list by the author a number of times. (Most recently regarding my questions on replacing Silicone Diff pump oil).
I am looking for the Title , Author, Publisher, etc. of this book if anyone could provide this info I'd greatly appreciate it.
} Date: 8/2/95 4:14 AM } From: Smuts, L } } Dear USERHHXS and identifiable microscopists: } } Many years ago, (20+) we had such a device on a PGT detector. It was } } nothing but trouble!!!!! The internal pressure in a 160L LN2 tank } } is....
} Can the cooling down of the PGT EDS crystal also be done with } liquid air (LAir) instead of Liquid Nitrogen (LN2)? } Boilingpoint: LN2=-195.8 C ; LAir=?differs with a few deg C from LN2. } Specific heat: LN2=0.2438 ; LAir=0.2374. (constant pressure)
} \\\\\\\\\\\\\\\\\\\\\} WWW: } http://www.puk.ac.za} \\\\\\\\\\\\\\\\\\\\\\} } Leon Smuts-Electronmicroprobe-University of Potchefstroom-South Africa} } ///////////////////} e-MAIL: plbls-at-puknet.puk.ac.za} ///////////////////}
------------------------------------------------------------- Hazard Warning --- -------------- The problem with an auto-fill system using liquid air is the fractional distillation effect that gradually enriches the oxygen content of the remaining cryogenic liquid as the liquid nitrogen evaporates preferentially. After a few weeks the oxygen-enriched liquid can be hazardously explosive! (I seem to remember a game that involved soaking cotton-wool in the liquid and igniting it with a Tesla coil . . . .). ------------------------------------------------------------- Michael A. O'Keefe Acting Head NCEM maok-at-lbl.gov
I'm looking for a source of the plastic sheet material made of ACLAR (TM?). A source of slides and/or coverslips made of the stuff would be ideal, but I will go for anything that might be used in lieu thereof. Also, if anyone has experience using this material for TEM embedding, I would be interested in their technique and opinion. Thanks in advance for any help you can give me.
Dan
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% % % % Daniel Possin Work: 206/ 543-7489 % % Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 % % University of Washington Home: 206/ 778-1714 % % Seattle WA 98195 USA Email: oemlab-at-u.washington.edu % % % % "The chinese expression 'cheung meng ba sui, gong hey fat choy' is % % equilvalent to Vulcan expression 'live long and prosper'. It's a % % small universe and getting smaller everyday". % % % %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
______________________________________________________________________________ San Joaquin Delta College World Wide Web:http://www.sjdccd.cc.ca.us 5151 Pacific Avenue email:webmaster-at-ms.sjdccd.cc.ca.us Stockton, CA 95207 general information:(209) 474-5151 or FAX-at-(209)474-5600
We have fitted ImageSlave digitising boards to 4 of our microscopes including a JEOL SEM 840. They fit in a PC 8 bit slot. The software is very friendly. We have it fixed so every time the PHOTO button is pressed an image is digitised concurrently with (or instead of) exposing a film or Polaroid image. The system then prompts for a filename and saves on the local net to our server. Will do automatic increments of file numbers. Currently runs under DOS but a windows version with TWAIN compliance is promised soon. Resolution currently 1024x1024x12 at Australian Dollars $3500 but 2048x2048x12 is promised soon
Distributors are world wide. I have a list if it is of wider interest.
ImageSlave distributors for the USA as of 1-Aug-95:
Contact Jim Hilton Advanced Database Systems 7931 S. Broadway #322 Littleton CO 80122 U.S.A. Tel: + 1 303 761-5635 Fax: + 1 303 761-592
We are not commercially involved, just happy customers. Mel Dickson.
The Alcor Foundation, a 501c(3) nonprofit organization located in Scottsdale, Arizona, needs to acquire a working TEM and/or SEM for use in cryobiological research. Are there any old instruments that someone would be willing to donate to us? Such a donation would be tax deductible. Thank You, Mark A. Voelker, PhD Alcor Foundation 7895 E. Acoma Drive Scottsdale, AZ 85260 telephone (602)922-9013
Mark Voekler SETS Technology Inc. 300 Kahelu Ave.,Suite 10 Mililani, HI 96789 Vox: 808-625-5262 Fax: 808-625-2474
Does anyone have a reliable technique for preparing human cilia, in particular dynein arms, for transmission electron microscopy? This is in cases of query immotile cilia syndrome.
Thanks in advance, Zyg Poczwa
Please reply to: richard.easingwood-at-stonebow.otago.ac.nz
South Campus Electron Microscope Unit Otago Medical School PO Box 913 Dunedin NEW ZEALAND
} liquid air (LAir) instead of Liquid Nitrogen (LN2)? } Boilingpoint: LN2=-195.8 C ; LAir=?differs with a few deg C from LN2. } Specific heat: LN2=0.2438 ; LAir=0.2374. (constant pressure) } } } Leon Smuts-Electronmicroprobe-University of Potchefstroom-South Africa} } } ///////////////////} e-MAIL: plbls-at-puknet.puk.ac.za} ///////////////////} } } I've never sat down and thought about this in detail to see if it's true, but I was told once upon a time, when the liquid nitrogen machine was down and we had to use liquid air, that it was fine for a short while, but that if you kept on using liquid air, you could accumulate liquid oxygen in your dewar - not desirable!
-- Keith R. Hallam | Owner of two cats, an '86 MR2, two melodeons, Research Associate | member of Rag Morris and Bristol Fashion and | a surface analyst, depending on the discussion University of Bristol, | group Interface Analysis Centre, | Telephone: National (0117) 925 5666 Oldbury House, | International + 44 117 925 5666 121, St. Michael's Hill, | Facsimile: National (0117) 925 5646 Bristol, | International + 44 117 925 5646 BS2 8BS, | E-mail: k.r.hallam-at-bristol.ac.uk England | URL: http://zeus.bris.ac.uk/~phkrh/
I've done this in dogs, humans etc once in a while.
I usually split the sample:
a. Std TEM, using Uranyl Acetate en block staining--saturated solution (aq) for 30-60 minutes
b. Std TEM with Tannic acid enblock staining. -----------------------------------------------------------------------
Microscopic Imaging Laboratory Embedding
Tannic Acid Fix
Fixation and embedding Procedure for Ciliated Samples to Visualize Protofilaments and Dynein Arms.
Special reagents needed:
0.1 M PBS, 0.5% Triton X-100 buffer:
1. Dilute stock(10%) TritonX-100 1/10 with water to make a final solution of 1% Triton X-100
2. Use one part 0.2M PBS buffer added to one part of 1% Triton X to make the final solution.
4% Tannic Acid in Buffer:
0.4 grams of EM grade Tannic Acid
10 mls of 0.1M Triton X buffer ___________________________________ *** Make fresh with every use.
Embedding:
1. After fixing in EM fix or Karnovsky's, rinse with buffer.
2. Incubate tissue in the 0.5% triton X /tannic acid solution for 1 hour, rotating at room temperature.
3. Remove solution, rinse with buffer.
4. Incubate in EM fix or Karnovsky's for 1 hour.
5. Rinse in buffer.
6. Incubate 2 hours in OsO4, Omit any usage of KCN or UA.
7. Continue as in standard embedding with dehydrations and infiltrations.
Reference:
Silsman, N.J. / C.E. Famum and D.K. Reed Variability of ciliary ultrastructure in normal dogs. ----------------------------------------------------------------------
It also sometimes helps to have a tilt mechanism on the TEM to bring cross sections of cilia into better focus alignment.
Hope this helps, use EM Grade Tannic Acid and make it fresh.
Lou Ann
} Does anyone have a reliable technique for preparing human cilia, in } particular dynein arms, for transmission electron microscopy? This is in } cases of query immotile cilia syndrome. } } Thanks in advance, } Zyg Poczwa } } Please reply to: richard.easingwood-at-stonebow.otago.ac.nz } } South Campus Electron Microscope Unit } Otago Medical School } PO Box 913 } Dunedin } NEW ZEALAND } } Telephone: 64-03-479 7301 } Facsimile: 64-03-479 7254
*********************** Lou Ann Miller Microscopic Imaging Laboratory College of Veterinary Medicine University of Illinois 2001 S Lincoln Ave Urbana, Illinois 61801 217-244-1566 lmiller-at-ux1.cso.uiuc.edu
Several times ther have been requests for information of fee structures of university based service laboratories,, doing EM . Has this information been summarized and archived somewhere where I might get to it? I do not want to request information that might already be available.
Thanks in advance, Greg -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- -at- Greg Erdos Phone: 904-392-1295 -at- -at- Scientific Director, -at- -at- ICBR Electron Microscopy Core Lab -at- -at- 218 Carr Hall Fax 904-846-0251 -at- -at- University of Florida E-mail: gwe-at-biotech.ufl.edu -at- -at- Gainesville, FL 32611 -at- -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Microscopists: We are shopping for a color/monochrome printer to hook to our in-house microscopy network, which is Appletalk. The printer would be used for color output for poster presentations and for monochrome output from the Mac hooked to our scanning EM. I hear the color output from the stylus is spectacular. How good is the *monochrome* output?
TIA Julian Smith III Biology Winthrop University Rock Hill SC 29733 smithj-at-winthrop.edu
Colleagues, I have been approached by a colleague whose research focuses on the physiology of a species of clam that lives in high-sulfide environments. She is investigating the function of the mitochondria in these particular organisms and wants to do some correlative morphology work. I am willing to help her out but have never in my life had to deal with a mollusk. Is there anything peculiar about fixation, tissue processing ect. that I should be aware of before undertaking this project?
Thanks in advance,
Kevin Kevin McCarthy Assistant Professor Department of Cell Biology Digital Imaging Microscopy Facility University of Alabama at Birmingham Birmingham, Alabama 35294 Phone 205-934-9923/9924 Fax 205-934-7029 "Seeing the World Through Different Eyes"
Neuroscience List {neur-sci-at-net.bio.net} , Cell Bio List {cellbiol-at-net.bio.net} Message-Id: {Pine.3.05.1.9508030803.A2104-9100000-at-pauline.sdsc.edu} Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII
Please excuse if slightly off topic:
What are the best culture vessels for sending live cell cultures through the mail to avoid cell damage? Thanks so much.
Marc
Marc C. Brande, M.S. SD3D Email List:3D Imaging San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu 3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830
Message-Id: {1995Aug03.093442.1158838932-at-ms.sjdccd.cc.ca.us} To: microscopy-at-aaem.amc.anl.gov (MSA list), smithj-at-acad.winthrop.edu (smithj)
Monochrome output is also very good. I use it for both SEMs and TEMs.Black are a true black. Cartridges are separate i.e. CYMK and BW. You can order special glossy paper which should give it a photographic look. The resolution is already photographic but with glossy paper it should be super. Regular glossy paper does not work as I have tried it. Good luck, Judy M PS At the price you may want to buy two. They are slow for 720 depi printing but well worth the wait.
Judy Murphy, PhD Dept. of Microscopy San Joaquin Delta College 5151 Pacific Ave Stockton, CA 95207
Microscopists: We are shopping for a color/monochrome printer to hook to our in-house microscopy network, which is Appletalk. The printer would be used for color output for poster presentations and for monochrome output from the Mac hooked to our scanning EM. I hear the color output from the stylus is spectacular. How good is the *monochrome* output?
TIA Julian Smith III Biology Winthrop University Rock Hill SC 29733 smithj-at-winthrop.edu
______________________________________________________________________________ San Joaquin Delta College World Wide Web:http://www.sjdccd.cc.ca.us 5151 Pacific Avenue email:webmaster-at-ms.sjdccd.cc.ca.us Stockton, CA 95207 general information:(209) 474-5151 or FAX-at-(209)474-5600
Dear colleagues I would like to get an idea of what the general use of sodium cacodylate is like "out there". My concern is that its arsenic content makes it an environmentally-unfriendly chemical. I discourage our users in favour of phosphate or "Good" buffers (PIPES, etc), and I am unaware of any shortcoming in ultrastructural preservation as a consequence of this. Furthermore, it is not a cheap buffer either.
In my opinion, the use cacodylate-based fixatives is justified for fixing specimens during field- trips, or whenever a buffer is to be kept at room temperature for a few days/ weeks since it will not become contaminated with bugs. However, I do have a problem with its wholesale use as a routine buffer.
What are your thoughts on the subject?
James Wesley-Smith Electron Microscope Unit George Campbell Building University of Natal Durban, South Africa
Julian- We are using the Stylus printer primarily for color images, but after reading your request I fired up the monochrome mode and printed a TEM image, the image is nice and for the price (1/10 or 1/20 of a dye sub print) it is fine. but if time is also included in the criteria, try out the 600 dpi laser printers HP-4 and/or Apple LaserWriter 16/600, the images are superior, and the machines are way faster. -Mike
On Thu, 3 Aug 1995 smithj-at-acad.winthrop.edu wrote:
} Microscopists: } We are shopping for a color/monochrome printer to hook to our in-house } microscopy network, which is Appletalk. The printer would be used } for color output for poster presentations and for monochrome output } from the Mac hooked to our scanning EM. I hear the color output } from the stylus is spectacular. How good is the *monochrome* } output? } } TIA } Julian Smith III } Biology } Winthrop University } Rock Hill SC 29733 } smithj-at-winthrop.edu }
I have used cacodylate buffer in the past mostly for the convenience of being able to make up a 0.2M cacodylate stock and store it long-term in the refrigerator, knowing it will be there ready for use. In contrast, in my experience, the usual 0.2M phosphate stocks at refrigerator temperature tend to crystallize out over time, so when you are ready to make up a fixative you may face the annoying task of warming the stock and agitating to get the crystals back into solution. Phosphate stocks are also more vulnerable to infections by bacteria or fungi, and so you may be greeted by a thriving colony when you are ready to make up your fixative.
However, as safety requirements become more and more stringent, the convenience of cacodylate may become counterbalanced by problems of disposal.
A. Kent Christensen, University of Michigan, {akc-at-umich.edu}
----------------------------------
On Thu, 3 Aug 1995, Mr James Wesley-Smith wrote:
} Dear colleagues } I would like to get an idea of what the general use of sodium } cacodylate is like "out there". My concern is that its arsenic } content makes it an environmentally-unfriendly chemical. I } discourage our users in favour of phosphate or "Good" buffers } (PIPES, etc), and I am unaware of any shortcoming in ultrastructural } preservation as a consequence of this. Furthermore, it is not a } cheap buffer either. } } In my opinion, the use cacodylate-based fixatives is justified for } fixing specimens during field- trips, or whenever a buffer is to be } kept at room temperature for a few days/ weeks since it will not } become contaminated with bugs. However, I do have a problem with its } wholesale use as a routine buffer. } } What are your thoughts on the subject? } } James Wesley-Smith } Electron Microscope Unit } George Campbell Building } University of Natal } Durban, South Africa } }
Comments: Converted from PROFS to RFC822 format by PUMP V2.2X
Can anyone help me with a query ? I am trying to find a supplier of plastic microscope slide boxes that are light tight and large enough to store glass slides 76 mm x 39 mm. I need to store slides at -70C. I would be grateful for any help ? Many thanx.
Sarah Augood EMAIL:augood-at-bbsrc.ac.uk FAX:44-1223-836614
Another solution to the B&W delima is the new Lexmark Optra R which is a laser printer that prints at 1200dpi - it's fast and simple -just plug it in. They cost about $2500 with the memory necessary to print large 1200 dpi images.
I'm workin with ferritin from horse spleen (Sigma I) as an electron microscopic marker. Although it is easy to identify the particles in the micrographs, I would like to know the exact size of theses particles. Could you help me, please Thank you ============================================================================= Francisco Javier Hernandez Blazquez | Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-usp.br Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265 Departamento de Ciencias Basicas/Histologia| r. 278 Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 611689 CEP 13630-000 Pirassununga (Sao Paulo) | 55 11 8694831 BRAZIL | ==============================================================================
You didn't say whether they were marine, freshwater, or terrestrial molluscs...it makes a BIG difference. Aquatic molluscs are osmoconformers, maintaining a tonicity roughly equivalent to their environment. My experience with any marine invertebrate invariably finds it best to make up a fixative (usually GTA-Formaldehyde) in seawater. Seawater is actually a fairly good buffer. Freshwater molluscs are more of a problem. Their blood measures only about 30 mOsmol. The buffers that we use for EM are ineffective at that concentration. My best results have been to make up 2% GTA-2%Form. in pondwater. Since its not buffered, the pH should be monitored during fixation and adjusted appropriately. It tends to progressively lower during the fixation process, and it should be kept above 7.2. The reason is that some freshwater molluscs, particularly bivalves contain huge numbers of calcium phosphate spherulites, which are rapidly lost under acidic conditions, leaving large empty spaces in the cytoplasm.
For a reference, see:
Steffens, et al. 1985. Can. J. Zool. (63): 348-353.
Good luck!
-=W.L. Steffens=- Department of Veterinary Pathology College of Veterinary Medicine University of Georgia
} I'm workin with ferritin from horse spleen (Sigma I) as an } electron microscopic marker. Although it is easy to identify the } particles in the micrographs, I would like to know the exact } size of theses particles. Could you help me, please } Thank you } ============================================================================= } Francisco Javier Hernandez Blazquez | } Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-usp.br } Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265 } Departamento de Ciencias Basicas/Histologia| r. 278 } Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 611689 } CEP 13630-000 Pirassununga (Sao Paulo) | 55 11 8694831 } BRAZIL | } ==============================================================================
Ferritin was introduced by Singer & Schick 1961 as a marker. It is a spherical molecule, 12 nm in outer diameter and a molecular weight of 750,000. It has an iron content of 23% by weight. The iron is concentrated in micelles in the center of the molecule forming a tetrad with a diameter of 5.5-6.0 nm which is the electron dense core. I hope this is what you need. Sverker
********************************************************* Sverker Enestrom M.D., Ph.D. Department of Pathology University of Linkoping, Sweden Phone: +46 13 22 15 20 Fax: +46 13 13 22 57 *********************************************************
Despite its toxicity, cacodylate buffer is not always resistant to microbial growth. Several years ago, we had something get into our large volume of cacodylate stock. It gave off a strong garlic smell, and was teeming with bacteria. One of our microbiologists found it to be a "demethylating bacterium". Cacodylate, being dimethyarsenic acid is actually food to some microorganisms. Thereafter, I began "poisoning" my stocks with sodium azide. So, in reality, resistance to microbial growth is not a factor in considering its use.
-=W.L. Steffens=- Department of Veterinary Pathology College of Veterinary Medicine University of Georgia
Our major use of cacodylate buffer is for enzyme cytochemistry. Lead based precipiation reactions are not compatible with phosphate buffers. However, we also find that for most enzyme cytochemistry cacodylate buffer gives superior results. For immunocytochemistry, on the other hand, Gey's salts is our preferred solution.
Jay Jerome ************************************************************** * aka: W. Gray Jerome * * Dept. of Pathology * * Bowman Gray School of Medicine of Wake Forest University * * Medical Center Blvd * * Winston-Salem, NC 27157-1092 * * 910-716-4972 * * jjerome-at-isnet.is.wfu.edu * **************************************************************
We are having problems staining photoreceptor outer segments in tissue embedded in JB4. We need to get differential staining of inner and outer segments for morphometric analysis. We've tried staining with tol blue which is our standard stain for epon embedded sections. Unfortunately, we do not get differential staining in JB4.
Does anyone out there have any suggestions? Since this may not be of general interest to the group, please e-mail me directly.
Marc C. Brande, M.S. SD3D Email List:3D Imaging San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu 3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830
On Fri, 4 Aug 1995 Microbill-at-aol.com wrote:
} Another solution to the B&W delima is the new Lexmark Optra R which is a } laser printer that prints at 1200dpi - it's fast and simple -just plug it } in. They cost about $2500 with the memory necessary to print large 1200 dpi } images. } } Bill Miller } ElectroImage
The problem as following is boring me. It will be very kind of you to give your hands to me if you had experienced such a kind of problem, or can give me some suggestions.
An FEG JEOL2010F microscope was used. On some exposed negative films, the information about magnification and scale bar were lost, i.e. they disappeared at all. However, the information about voltage and text were still there. I checked the history of the exposed films from the microscope CRT. There were no the lost information, either.
Many of you will recall that at last years Computer Workshop & Software Exchange held at the MSA/MAS meeting in New Orleans we ran a round robin test of gray scale printers.
I have kept all of the output submitted by everyone to the Computer Workshop and will be bringing all those prints with me back to this years meeting. They were all stored identically in a file cabinet for a year. We can all view how well each of these prints survived.
I would invite everyone that brought a print to the meeting to once again print a fresh copy, so that we can compare last years output with a "fresh" copy.
The test images are available via Anonymous FTP from the site:
Host: WWW.AMC.ANL.GOV (146.139.72.10) User: Anonymous Pass: Your Email Address
all are BINARY TIFF files. The _MAC file have Mac Byte order while the _IBMPC have PC Byte order.
Remember to download them as BINARY!!!!!
See you all in Breckenridge Aug 6-11 (MAS Meeting) or in KC on August 14th-17th (MSA/HCS Meeting)!!!
I will be at both meetings.
Hopefully, the listserver will run in relative peace during my absence. However, we all know that Murphy says the system will go nuts about the time I get on the plane...
I've tried being polite by sending this message to listserver-at-aaem.amc.anl.gov, but I am still getting microscopy mail. So I am sorry that this list server does not work well enough to handle this simple function, forcing me to broadcast such junk.
I find that this list covers too broad a subject area, and so generates too much mail for which I have no interest. I would rather subscribe to a usenet group dedicated just to SEMs.
August 4, 1995: Job Opening: Senior TEM Lab Engineer, Intel Corporation, Santa Clara, California.
---------------------------------------------------------------- Requires two or more years hands-on industrial experience with the use of Transmission Electron Microscopy and related techniques in the solution of semiconductor technology problems. Ideally this will include a number of years of experience working with development and manufacturing engineers in process characterization and debug, yield improvement and failure analysis. Supervision experience and demonstrated communication and interpersonal skills are necessary.
The individual will carry out TEM and other materials characterization analyses in response to customer requests, and will be a key interface with the customers in determining priorities in an environment where the volume of analytical requests frequently exceeds the available laboratory resources. The individual will participate in both task force and more routine problem solving efforts with our customer base as an active team member. The individual will interpret and make recommendations based on lab results and process knowledge. The individual will be responsible for the development of improved sample preparation techniques, overall TEM capability improvement and related analytical method development. The individual will work with lab peers in continuous improvement of lab technical capability and efficiency. The individual will supervise engineers and technicians in carrying out these responsibilities.
A Ph.D. in Materials Science or equivalent is required.
Intel is an equal opportunity employer.
Please mail resumes to:
John Mardinly Intel Corporation 2200 Mission College Blvd. Mail Stop SC2-24 Santa Clara, CA 95052-8119
and/or FAX to: (408)756-2393
Due to the large number of responses anticipated, there will generally be no replies unless the applicant is chosen to be a candidate.
Professor Liu Yong Kang is in charge of the 14th International Congress on X-ray Optics and Microanalysis, which will be held on August 29 -- September 2, 1995 in Guang Zhou, China.
His fax is : (86) 20 5514 130 His telphone is :(86) 20 5519 755 ext. 2234
He does not have an email service.
I would like to pass all the email messages from the net to him by fax inside China, if some one uses the net to pass the congress messages. It would be fast and reliable also. And please do not worry about the cost of the fax inside China, it is cheap and not much.
Welcome to China.
Zhen Quan Liu zqliu-at-pku.edu.cn Fax: (86) 10 250 1615
In message , writes:
} } Dear All, } Does anyone know the E-mail of the secretariat of the ICXOM - } The 14th International Congress on X-ray Optics and Microanalysis? } ICXOM will be held on Aug.29 - Sept.2, 1995 in the Guangzhou, China. } I very need the fast communication with the Organizing Commitee } and the post is too slow. } Many thanks! } } Sergey Kramar } CFPM, Moscow Institute of Electronic Technology, } Moscow 103498, Russia } E-mail: lemi-at-mx.iki.rssi.ru (to S.Kramar) } !!!! } !!!! } Received: from [162.105.160.2] by pccms.pku.edu.cn with SMTP id AA00464 } (5.67b8/IDA-1.5 for zqliu); Wed, 2 Aug 1995 16:43:43 +0800 } X-Nupop-Charset: English } Date: Wed, 2 Aug 1995 16:54:48 -0600 (CST) }
I am collecting Web links to HREM related and/or active sites. Please send me by email your link if it is not already included in the list at http://risc1.numis.nwu.edu/other.html
I may have inadvertantly given the wrong name when I requested HREM sites on the Web. The correct location for the current (incomplete listing) is: http://risc1.numis.nwu.edu/internet/other.html
Nine years ago, we purchased an Edwards model CR160P/E2M12 evaporator with an LN2 trap for a specific application. If anyone is interested in it, please call Paul Kenney at 800-992-9037, or e-mail me directly. Steven Slap, Energy Beam Sciences
We purchased a Zeiss Ultraphot II some years ago, and no longer have any use for it. It is in good working order, and we have the manual and other documentation. Please e-mail me directly for further details. Steven Slap, Energy Beam Sciences
Well I should have known better. If you areall wondering why there were no positngs for a few days, the system decided to hang on Sunday. Checking in tonight from the MAS meeting in Breckenridge Co, I restarted the Mail server. You should all be getting mail again.
Dear everyone, I'm searching for the Debye temperature of MgO, but I can't find any reference. I would like to do some temperature dependent EXELFS- calculations on MgO and compare them to experimental data.
I would be very pleased, if someone could send me a message (Debye temperature and reference, where to find it in the literature).
Message-Id: {9508071547.AA00619-at-unlinfo.unl.edu} X-Sender: tvoiles-at-129.93.1.11 (Unverified) X-Mailer: Windows Eudora Version 1.4.4 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
I was just wondering, someone mentioned a roundtable discussion happening at the MSA meeting in KC next week concerning the managing of EM labs. Is this still going on? I have the program book and don't see it anywhere.
Thanks
Todd Voiles Laboratory Manager Central Facility for Electron Microscopy Center for Materials Research and Analysis
I have read that when making up paraformaldehyde solution one should avoid HCl when adjusting the pH. Supposedly, HCl reacts with PFA to evolve a "potent carcinogen". Comments? Could the carcinogen be formaldehyde gas?
Here's the reference.
Jackson, D. (1991). "In-situ hybridization in plants." Molecular Plant Pathology A Practical Approach Eds Bowles D.J., S.J Gurr and M McPherson(Oxford University Press.).
Steve...
_______________________________ Steven Ruzin NSF Center of Plant Developmental Biology University of California Berkeley CA 94720-3102 510-642-6602
I am looking for the phone number for Oncor Instrument Systems, formerly of San Diego. I have a seven year old image analysis system they made that is running great but I need a file conversion program and they are no longer at their old address. TIA
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility 3 Tucker Hall University of Missouri Columbia, MO 65211 (314)-882-4712 (voice) (314)-882-0123 (fax)
Sender: cemerson-at-KEAN.UCS.MUN.CA {cemerson-at-kean.ucs.mun.ca} Reply-To: cemerson-at-KEAN.UCS.MUN.CA To: MICROSCOPY-at-AAEM.AMC.ANL.GOV Message-ID: {0099485E.950F3644.4905-at-leif.ucs.mun.ca}
Our lab owns a Zeiss EM 9A TEM purchased in the mid 60's. It is still functioning well, with a few quirks of personality here and there (as with us all!!), but we've recently had some damage to our specimen holders. We were wondering if anyone out there haas an old Zeiss EM 9A that is being cannibalized or is ready for the scrap heap and has some parts to spare. Specifically, we would like to scrounge or buy a specimen holder. If you have one, could you please contact me off-line with the details? Thank you. Carolyn J. Emerson Dept. of Biology Memorial Univ. of Newfoundland St. John's, NF, Canada A1B 3X9
Microscopy Online is happy to announce its server on the WWW at
http://www.microscopy-online.com/
Microscopy Online is a hyper-journal containing information and features of interest to the microscopy community, including articles, job listings, calendar of events, keyword-searchable buyer's guide, showcase of new products and services, list of recent publications, used equipment for sale, etc.
We invite to you take a look and welcome your comments and ideas for improvements.
Regarding the problem with disappearing scalebar and maginfication on negatives: We have had the same problem with our JEM4000-EX for over a year now. It started when we installed a Gatan CCD-camera with Digital Micrograph and autoalignment. The scalebar and mag. sometimes disappears during a session, but it doesn't seem to be any special event that causes it. However, the annotation usally comes back when we reset the microscope computer. Jeol Scandinavia has so far not been able to solve the problem.
I don't know if this is of any help, but at least you know that your not the only one having this problem.
Best Regards Anna Carlsson National Center for HREM Lund University, Sweden
Todd et al.: The Technologists' Forum roundtable, entitled "Survival Part III: What Works and What Doesn't", will be held Thursday from 1-3 PM in Room 1201 at the Convention Center. It is on page 45 of the program. If you have any questions, please contact me this week, or come to the Tech Forum booth in the exhibit hall at the meeting. Hope to see you there.
Bev Maleeff
Todd Voiles wrote: I was just wondering, someone mentioned a roundtable discussion happening at the MSA meeting in KC next week concerning the managing of EM labs. Is this still going on? I have the program book and don't see it anywhere.
Thanks
Todd Voiles Laboratory Manager Central Facility for Electron Microscopy Center for Materials Research and Analysis
After my post on cacodylate buffer, I have had a number of people ask me what is Gey's salts. So I thought I would share this information with the entire list. Gey's salts is a balanced salt solution, similar to Hank's. A recipe can be found in most tissue culture handbooks. We have found that preserves antigenicity in most proteins better than EM buffers or other balanced salt solutions. It has only a limited buffering capability, so pHing can be difficult. We use very dilute NaOH and HCl to alter it's pH.
Hope this helps.
Jay Jerome ************************************************************** * aka: W. Gray Jerome * * Dept. of Pathology * * Bowman Gray School of Medicine of Wake Forest University * * Medical Center Blvd * * Winston-Salem, NC 27157-1092 * * 910-716-4972 * * jjerome-at-isnet.is.wfu.edu * **************************************************************
On Tue, 8 Aug 1995, PRING wrote:
} I note your comments on cacodylate - what are grey's salts? } } Richard } } richard.pring-at-bbsrc.ac.uk } } Richard Pring } Long Ashton Research Station } Long Ashton } Bristol } UK }
I'm looking for any advice or references on making plan-view (or cross section) specimen of films grown on sapphire. From what I've read, the samples can be make using tried and true methods used commonly on semiconductor substrates (polish/dimple/ion mill). Does any one know any helpful hints or 'tricks' to make this process go as smoothly as possible? I am also thinking of chemical etching for plan-view specimen, but I have the usual problem of how to know when to stop etching. Any suggestions?
Joyce Palmer University of Massachusetts ECE department Amherst, Ma. 01003 413-545-4647
First, my apologies to those who read the NIH-image listserver, as this is a duplicate of a posting I made there earlier today.
I am collecting information on 1200dpi monochrome laserprinters for printing half-toned grayscale images of scanned TEM and HRTEM photographic prints. We want the capability to print output from Photoshop and NIH-Image onto paper and transparencies for seminars and progress reports without degrading the image quality. Presently, we resort to reproducing the photographs with the monochrome mode on a Cannon color copier to preserve as many gray levels in the final output. This approach, however, does not allow direct output from the computer, which in our case is a PowerMac 7100/80.
I would appreciate responses from anyone who has had any experience with the following 1200dpi laserprinters:
I would also appreciate any pointers to review articles, comments about gray-scale printing, interface pitfalls, and other 1200dpi models available (Please include phone # or web sites).
Thank You,
David A. Howell MSM Dept. Michigan State University E. Lansing, MI 48824-1226
The rountable on EM Facility Management will be on Thursday, 1-3PM, August 16, Room 1201. Topic: Survival Part Three: What Works and What Doesn't.
-------------------------- Earlier, Tod Voiles Wrote:
} I was just wondering, someone mentioned a roundtable discussion happening at } the MSA meeting in KC next week concerning the managing of EM labs. Is this } still going on? } I have the program book and don't see it anywhere. } --------------------------
############################################################################# John J. Bozzola, Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901-4402 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html #############################################################################
To users of Lowicryl resins: I have just opened two kits of Lowicryl K11M, both with the same lot number from the same supplier. In one of the kits the monomer is a pale straw colour (abnormal) while in the other kit the monomer is colourless (normal). The pale straw colour is a bit disconcerting as I am used to the colourless momomer. In addition the blocks made from the pale straw coloured monomer seem to polymerise slightly harder than the colourless ones however I have no idea if the discoloured resin effects immunoreactivity. To date Polysciences have not been able to explain the colour difference. Has anyone out there had the same experience? If so do you have any ideas on what causes it? Do you think it affects immunoreactivity?
Allan Mitchell
Please reply to: richard.easingwood-at-stonebow.otago.ac.nz
Richard Easingwood South Campus Electron Microscope Unit Otago Medical School PO Box 913 Dunedin NEW ZEALAND
We are about to start an experiment which involves human platelets for a user of the Unit. He wants to look at the platelet structure before aggregation occurs. Aggregation of platelets can occur during platelet isolation but in particular during chemical fixation. I am proposing to cryofix and cryosubstitute the platelets. Has anybody had experience with platelets and this technique who would like to share that experience?
Many thanks,
Allan Mitchell
Please reply to: richard.easingwood-at-stonebow.otago.ac.nz
Richard Easingwood South Campus Electron Microscope Unit Otago Medical School PO Box 913 Dunedin NEW ZEALAND
Comments: Converted from PROFS to RFC822 format by PUMP V2.2X
Dear Fellow Microscopists, Could someone please forward the recent listing concerning the microscopy-online service as seen by this listserver. with thanks David Dryden School of Physics University of Melbourne Australia djd-at-electron.ph.unimelb.edu.au
I would be grateful of any advice on the best way to polish, in a Fischione twin jetpolisher, a Au-Cu-Al alloy in which gold is present at about 50%atomic. Thanks
Dr MJ Witcomb Electron Microscope Unit University of the Witwatersrand Private Bag 3 WITS 2050 South Africa
I am currently using an old Ion Tech B306 ion mill to produce foils for TEM microanalysis. I would like to ion mill specimens at liquid nitrogen temperatures but, unfortunately, I do not have access to a cold stage. Furthermore, my budget is constrained and will not stretch to the cost of buying a new one. I would, however, be interested in hearing from anyone who has an Ion Tech cold stage which is superflous to their requirements and which they would be willing to sell (or even perhaps part exchange for other Ion Tech Parts).
Thank you, *************************************** ************ Lee Smith ************ ******* School of Materials ******* ******* University of Leeds ******* ******* Leeds, LS2 9JT, UK ******* ***************************************
Does anyone know if there exists a high resolution film scanner that will scan 31/4x4 and 4x5 negatives in addition to 35mm? I have a brochure for the Nikon LS-3510AF and it looks like the largest negative it will scan is 40mmx40mm.
If such a scanner does exist, what is the quality of the scanned image? I would like to output the scanned image (after manipulation in Photoshop) to a Codonics dye-sublimation color printer or film recorder.
11th September 1995 at Department of Materials Science & Engineering, University of Liverpool, UK
This workshop is timed to take place immediately before EMAG 95 at Birmingham (12th-15th September). It provides an unique opportunity to see a VG HB601UX high resolution analytical STEM in operation and to discuss problems with regular users of the instrument.
There will be four sessions, and plenary lectures by speakers from the USA, UK and Germany, including Steve Pennycook on Z-contrast imaging and and Ian Vatter on high spatial resolution analysis.
You can register (there is no fee!) via Anne Leonard at Fisons (44) 1342 327211, Fax (44) 1342 300515 or via the Fisons Web page at http://www.surface.fisons.co.uk/workshop
The instrument at Liverpool is the North-West STEM, jontly run by the Universities of Manchester and Liverpool.
Peter Goodhew
---------------------------------------------------------------- Professor Peter J Goodhew Department of Materials Science & Engineering University of Liverpool LIVERPOOL Fax (44) (0)151 794 4675 L69 3BX, UK Tel (44) (0)151 794 4665 (secretary Debra) ---------------------------------------------------------------- inter alia:
Director of the MATTER project for educational software Web page: http://www.liv.ac.uk/~matter/home.html Tel (44) (0)151 794 5006 (secretary Jean)
Dean of Engineering Tel (44) (0)151 794 4920 (secretary Carol) Fax (44) (0)151 794 4930 ----------------------------------------------------------------
Message-Id: {9508091356.AA29780-at-unlinfo.unl.edu} X-Sender: tvoiles-at-129.93.1.11 X-Mailer: Windows Eudora Version 1.4.4 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
} Hi everybody, } } Does anyone know if there exists a high resolution film scanner that will } scan 31/4x4 and 4x5 negatives in addition to 35mm? I have a brochure } for the Nikon LS-3510AF and it looks like the largest negative it will } scan is 40mmx40mm. } } If such a scanner does exist, what is the quality of the scanned image? } I would like to output the scanned image (after manipulation in } Photoshop) to a Codonics dye-sublimation color printer or film recorder. } } Thanks for any input. } } There are plenty of commercially available 1200 dpi and even 2400 dpi scanners out there for 1-5k. Is this enough resolution for what you want to do? They can usually scan in excess of 8x10 inches.
Todd Voiles Laboratory Manager Central Facility for Electron Microscopy Center for Materials Research and Analysis
} Hi everybody, } } Does anyone know if there exists a high resolution film scanner that will } scan 31/4x4 and 4x5 negatives in addition to 35mm? I have a brochure } for the Nikon LS-3510AF and it looks like the largest negative it will } scan is 40mmx40mm. } } If such a scanner does exist, what is the quality of the scanned image? } I would like to output the scanned image (after manipulation in } Photoshop) to a Codonics dye-sublimation color printer or film recorder. } } Thanks for any input. } } Ilene--
One film scanner to look at is the Leafscan 45, which does any size up to 4X5. It is a bit pricey, $15K when we looked a year ago, but it does a very nice job. It uses the same round negative carriers as Beseler enlargers, which are cheaper than the Leaf brand.
I don't remember the resolution, but, if it weren't for the price, I would have pushed for getting this scanner for our TEM negatives. It has automatic exposure control that gives very good results.
I'd like to see a vendor do some demos of these devices at MSA in Kansas City. Any takers from the vendors out there?
I am investigating the feasibility of purchasing a digital camera for use in our department to put on a microscope (probably an Olympus Vanox) and am trying to understand the advantages/disadv. of a dig. camera over video or scanning 35mm slides since the cost seems to be so high.
1) Video vs. digital cameras: Video CCD cameras can be put on microscopes and can capture a pretty good quality image, requiring a video capture board for use on the computer. The image quality is limited, however, by the resolution of the CRT. The digital cameras should be able to improve on the resolution in theory , but what does the image actually look like? Fuji says their image only holds up to a 3x5 enlargement... Is video still the better alternative for instant file use, and scanning 35mm slides still the best quality?
2) Digital Recommendations: Fuji says their camera isn't really ready for use on a scope, largely due to the incompatibility of the design of light capture in a digital system and the idiosyncrasies of a microscope, specifically the camera's reliance on the autofocus lens for metering versus an objective and the camera's limit of ISO values of only 800 and 1600. So has anyone used a Kodak camera on a scope, or any other brand? What were the successes and problems with those you've tried? Is file format (.jpeg or .tif versus a proprietary format that needs to be converted for use in a real application) an issue that has been a problem? What are you doing for storage? Kodak's Digital imaging helpdesk has not been able to link me up yet w/ anyone in the company that knows anything about their cameras on a microscope...
Any comments or light that you can shed on this subject would be greatly appreciated. If it would be easier for you to talk with me on the phone, please send your phone number and I'll call you. THanks in advance for your time to reply -
Susan Reeves Supervisor, PhotoPath DUMC Department of Pathology reeve008-at-mc.duke.edu 919-684-3984
Ilene: We use a LeafScan-45 for negatives from 35mm to 4"x5" controlled by a Mac (via SCSI, but you could use GPIB). It uses standard Beseler enlarger film carriers, has a plug-in for Photoshop, and scans up to 6000x12000 pixels (216MB file for color -- 72MB for grayscale!). The maximum dpi is 4000, so the minimum size to get 6000 pixels across is 1.5" (if you want to scan (say) a 10mmx10mm area, you get only 1575x1575 pixels). We bought the LeafScan (from Leaf Systems: 508-460-8300) as a replacement for an Eikonix 78/99, and are very satisfied with it. Mike O'Keefe --------------------------------------
Does anyone know if there exists a high resolution film scanner that will scan 31/4x4 and 4x5 negatives in addition to 35mm? I have a brochure for the Nikon LS-3510AF and it looks like the largest negative it will scan is 40mmx40mm.
If such a scanner does exist, what is the quality of the scanned image? I would like to output the scanned image (after manipulation in Photoshop) to a Codonics dye-sublimation color printer or film recorder.
While I cannot give you any help with using a Fischione Polisher, i can give you a recipe which is used with the South Bay Technology Model 550. Perhaps you can use this as a basis for a Fischione recipe.
Reference: B.J. Kestel, "Jet Thinning of YBaCuO High Tc Superconducotr and also Gold for TEM with a Non-Acid Electrolyte" Ultramicroscopy 25 (1988) pp 351-354.
If you cannot get a copy, I can send one to you.
He uses a BK-2 Solution which is: 5.3g lithium chloride 11.16g magnesium perchlorate 100ml butyl cellosolve 500 ml methanol
Polishing was done at -55C with a potential of 150V at one half the maximum flow rate.
I hope this helps. If you would like anyu additional information or a copy of the paper, please let me know.
Best regards-
David Henriks TEL: 800-728-2233 (toll-free in USA) South Bay Technology, Inc. 714-492-2600 1120 Via Callejon FAX: 714-492-1499 San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com
ROGER ALVIS {Roger.Alvis-at-amd.com} , "Ronald M. Anderson" {ron-anderson-at-vnet.ibm.com} , "Miguel Avalos B." {miguel-at-ifuname.ifisicaen.unam.mx} , Yolande Berta {YBerta-at-matreng.courier.gatech.edu} , Peggy Bisher {peggy-at-research.nj.nec.com} , henk colijn {colijn-at-kcgl1.eng.ohio-state.edu} , Russ Cook/Argonne {COOK-at-aaem.amc.anl.gov} , Mike Dibattista {MikeDib-at-engine.umich.edu} , Cindy Dogan {DOGAN-at-alrc.usbm.gov} , Estevez {estevez-at-atp6000.tuwien.ac.at} , Phil Flaitz-IBM {pflaitz-at-vnet.ibm.com} , Tim Foecke {tfoecke-at-nist.gov} , Richard Fonda {Fonda-at-anvil.nrl.navy.mil} , Chuck Garber {GVKM07A-at-prodigy.com} , Zack Gemmill {zack-at-lsil.com} , Lucille Giannuzzi {lag-at-pegasus.cc.ucf.edu} , Ed Goo {ekgoo-at-mizar.usc.edu} , Peter Goodhew {goodhew-at-liv.ac.uk} , Vidya Kaushik {QJXNJ21-at-memrqa.sps.mot.com} , Bernie Kestel {Bernard_Kestel-at-QMGATE.ANL.COM} , "Kim,SungTae(T:4551)" {STKIM-at-gscrl.goldstar.co.kr} , Michael Lamvik {mlamvik-at-mcnc.org} , "J.S. Lee" {JSLEE-at-gscrl.goldstar.co.kr} , Tan Chen Lee-Cornell {tanchen-at-msc.cornell.edu} , Microscopy ListServer {MICROSCOPY-at-aaem.amc.anl.gov} , Zhen Quan Liu {zqliu-at-pku.edu.cn} , Jian Shu Luo {jian_shu_luo-at-qmgate.anl.gov} , Charlie Lyman {cel1-at-lehigh.edu} , Jordi Marti {MartiJ-at-mtomp201.research.allied.com} , James McCormick {JAMESM-at-teetot.acusd.edu} , Stuart McKernan {mckernan-at-cems.umn.edu} , "F. Scott Miller" {smiller-at-umr.edu} , Lucio Mulestagno {LucioM-at-newton.umsl.edu} , Judy Murphy/SJDC {murphy-at-ms.sjdccd.cc.ca.us} , BOB ROBERTS {ROBERTS-at-csss.la.asu.edu} , Ludo Rossou {LUDO_GERTIE-at-ematserv.ruca.ua.ac.be} , Jake Schaper {Jake_Schaper-at-chdqm.sps.mot.com} , David Su {davidsu-at-aol.com} , Changmo Sung {Sungc-at-aspen.uml.edu} , Don Grimes/Micro Today {MicroToday-at-aol.com} , Scott Walck {WALCKSD-at-ml.wpafb.af.mil} , John Wheatley {WHEATLEY-at-csss.la.asu.edu}
South Bay Technology will present a tutorial on Tuesday night from 5-7pm in their exhibit booth (no. 619-621). The tutorial is FREE. All you need to do is register for it at the convention center. Look for the signs announcing Vendor Tutorials. If you have trouble figuring out how to register, just stop by our booth on Monday or Tuesday and we'll get you set up.
Presenters:
Trpiod Polishing: Shane Roberts Applications Engineer South Bay Technology, Inc.
Ion Milling: Dr. Arpad Barna Research Institute of Technical Physics Budapest, Hungary
"South Bay Technology will discuss the fundamentals of Tripod Polishing including a step by presentation of sample mounting, sample alignment, Tripod Polisher calibration, 1st side polishing and 2nd side (wedge) polishing. New sample mounts and accessories will also be introduced during the tutorial. The newest advances in Tripod Polishing will also be discussed which makes this tutorial ideal for both novices and experienced Tripodders. The ion milling portion of the tutorial will be an introduction to the IV3 Ultra Low Angle Ion Mill. The 1st production version of the IV3 was introduced in Hungary in 1987. Since that time the IV3 has enjoyed brisk sales throughout Europe and the Far East. This tutorial is an opportunity to learn more about the unique ion guns and the many benefits associated with its retarding field capability. Dr. Barna is a world reknowned expert on ion milling and developed the first working model of the IV3 in his lab in 1982."
ROGER ALVIS {Roger.Alvis-at-amd.com} , "Ronald M. Anderson" {ron-anderson-at-vnet.ibm.com} , "Miguel Avalos B." {miguel-at-ifuname.ifisicaen.unam.mx} , Yolande Berta {YBerta-at-matreng.courier.gatech.edu} , Peggy Bisher {peggy-at-research.nj.nec.com} , henk colijn {colijn-at-kcgl1.eng.ohio-state.edu} , Russ Cook/Argonne {COOK-at-aaem.amc.anl.gov} , Mike Dibattista {MikeDib-at-engine.umich.edu} , Cindy Dogan {DOGAN-at-alrc.usbm.gov} , Estevez {estevez-at-atp6000.tuwien.ac.at} , Phil Flaitz-IBM {pflaitz-at-vnet.ibm.com} , Tim Foecke {tfoecke-at-nist.gov} , Richard Fonda {Fonda-at-anvil.nrl.navy.mil} , Chuck Garber {GVKM07A-at-prodigy.com} , Zack Gemmill {zack-at-lsil.com} , Lucille Giannuzzi {lag-at-pegasus.cc.ucf.edu} , Ed Goo {ekgoo-at-mizar.usc.edu} , Peter Goodhew {goodhew-at-liv.ac.uk} , Vidya Kaushik {QJXNJ21-at-memrqa.sps.mot.com} , Bernie Kestel {Bernard_Kestel-at-QMGATE.ANL.COM} , "Kim,SungTae(T:4551)" {STKIM-at-gscrl.goldstar.co.kr} , Michael Lamvik {mlamvik-at-mcnc.org} , "J.S. Lee" {JSLEE-at-gscrl.goldstar.co.kr} , Tan Chen Lee-Cornell {tanchen-at-msc.cornell.edu} , Microscopy ListServer {MICROSCOPY-at-aaem.amc.anl.gov} , Zhen Quan Liu {zqliu-at-pku.edu.cn} , Jian Shu Luo {jian_shu_luo-at-qmgate.anl.gov} , Charlie Lyman {cel1-at-lehigh.edu} , Jordi Marti {MartiJ-at-mtomp201.research.allied.com} , James McCormick {JAMESM-at-teetot.acusd.edu} , Stuart McKernan {mckernan-at-cems.umn.edu} , "F. Scott Miller" {smiller-at-umr.edu} , Lucio Mulestagno {LucioM-at-newton.umsl.edu} , Judy Murphy/SJDC {murphy-at-ms.sjdccd.cc.ca.us} , BOB ROBERTS {ROBERTS-at-csss.la.asu.edu} , Ludo Rossou {LUDO_GERTIE-at-ematserv.ruca.ua.ac.be} , Jake Schaper {Jake_Schaper-at-chdqm.sps.mot.com} , David Su {davidsu-at-aol.com} , Changmo Sung {Sungc-at-aspen.uml.edu} , Don Grimes/Micro Today {MicroToday-at-aol.com} , Scott Walck {WALCKSD-at-ml.wpafb.af.mil} , John Wheatley {WHEATLEY-at-csss.la.asu.edu}
First let me say that this user's group meeting is not limited to those of you who have purchased a Tripod Polisher from South Bay Technology. We welcome ANYONE who has an interest in Tripod Polishing.
I'm sorry it has taken so long to get these final details on the User's Group Meeting. I hope you all took my advice and reserved the time in your schedule! We will meet as follows:
WHEN: Monday August 14th at 6:00 pm
WHERE: Gino Schiraldi's (Pizza Place) 323 W. 8th Street Kansas City, MO 64105 TEL: 816-421-2211
Gino's is located about 4 blocks from the convention center. Walk down Central to 9th and make a left. Then a quick right on May. Go 1 block to 8th and turn right. Gino's will be right there.
South Bay Technology will provide pizza and beer - so come hungry! It would also help me a great deal if you could RSVP. I'd like to get a rough idea of how many people will attend so we can reserve a large enough area.
It would be great if you could bring with you any photographs of your work, examples of Tripod Polisher modifications, copies of papers or procedures you have written etc. If you need (or want) to get in touch with me in Kansas City you can give me a call at the Marriott (816-421-6800). I'll arrive Sunday evening..
PLEASE let others know about the User's Group Meeting. I know I'm late in getting this information out so I'll need your in publicizing it.
Also, SBT will present a vendor tutorial on Tuesday night from 5-7 in the exhibit hall. The topics will be Tripod Polishing and Ultra Low Angle Ion Milling. The Ion Milling discussion will be led by Dr. Arpad Barna from the Research Institute for Technical Physics in Budapest, Hungary. He will discuss the IV3 Ion Mill which is being introduced to the U.S. market at MSA. The tutorial will be held in the South Bay Technology booth (619-621). We hope to see you there!
Best regards-
David Henriks TEL: 800-728-2233 (toll-free in USA) South Bay Technology, Inc. 714-492-2600 1120 Via Callejon FAX: 714-492-1499 San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com
MolecularCellSpeak List {molecular-cell-speak-at-mailbase.ac.uk} , Microscopy List {microscopy-at-aaem.amc.anl.gov} , Info-Bios List {info-bios-at-mom.spie.org} , Confocal Microscopy List {confocal%ubvm.BITNET-at-BITNIC.CREN.NET} , Cell Bio List {cellbiol-at-net.bio.net} , Biz-Biotech List {biz-biotech-at-netcom.com} , Bionews List {bionews-at-net.bio.net} , Biomaterial List {biomat-l-at-hearn} Message-Id: {Pine.3.05.1.9508091106.A25102-b100000-at-pauline.sdsc.edu} Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII
Is Your Unique Cell Image Data going Unrecorded?
There is a Solution!
Digital Imaging of Your Live Cells.
Capture Important Structures Functions and Interactions of Your Live Cells In Situ!
Monitor and Document Your Live-Cell Experiments Routinely and Frequently.
Why Digital Imaging?
Digital Images can be: Displayed Manipulated Processed Stored Retrieved Shared Transported
All via your Personal Computer!
No need to invest in expensive system hardware and imaging personnel
Ease of Use Safe Archival Image Storage Economical Fast Turnaround Performed by Live-Cell Imaging Biologist Meticulous Care Taken with Your Live Cells Complete Confidentiality Assured
To image your live cells, contact:
Marc C. Brande, M.S. or Caroline Yu at Cell Applications, Inc. Toll-Free: 1-800-645-0848 Local: 619-453-0848 Fax: 619-453-2862 Email: BRANDE-at-SDSC.EDU
Marc C. Brande, M.S. SD3D Email List:3D Imaging San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu 3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830
Customer is interested in drug toxicity of rabbit cornea. Is it best to fix in 3% Glutaraldehyde only or Glutaraldehyde (2.5%) and Paraformaldehyde (1.5%)? This is an anterior chamber study including the cornea endothelial cell layer.
The calculated Debye temperature of MgO is 630K, which can be found in { {The Oxide Handbook} } , 2nd edition, Ed. by G.V.Samsonov, published by IFI/Plenum Data Company (1982), p.109.
No experimental data of Debye Temp. for MgO is available in the book.
Alternate-Recipient: allowed Auto-Forwarded: prohibited Content-Return: allowed Disclose-Recipients: prohibited Conversion: allowed Importance: normal Priority: normal Sensitivity: Company-Confidential
POST-DOCTORAL POSITION IN BIOMEDICAL IMAGING:
Applications are invited for a full-time position as a Research Associate in the Department of Ophthalmology at the University of Texas Southwestern Medical Center at Dallas. We are seeking an individual with interest and experience in biological microscopy and computer imaging. The ideal candidate will have a Ph.D. in Biomedical Engineering, Cell Biology or related discipline. The proposed work will focus on the acquisition and quantitation of 3- and 4-dimensional images obtained from patients and experimental tissue using confocal microscopy. Responsibilities will include digitizing, processing, interpreting and analyzing images, as well as developing software as needed in order to generate reliable quantitative data. We use Silicon Graphics Workstations for image analysis (C or C++ programming languages).
Position is available immediately. Applicants should send a curriculum vitae with statement of interest to:
Dr. W. Matthew Petroll Department of Ophthalmology University of Texas Southwestern Medical Center at Dallas 5323 Harry Hines Blvd. Dallas, TX 75235-9057 FAX: 214-648-2382 EMAIL: petroll-at-crnmpsgi.swmed.edu
We have often cut cells still on thermanox coverslips. We use an older knife to be on the safe side, but they seem to cut o.k. The only problem we have is that the resin and cells sometimes separate from the thermanox as the sections come off. The sectioning is not easy, but it can be done and produces nice results.
Jay Jerome ************************************************************** * aka: W. Gray Jerome * * Dept. of Pathology * * Bowman Gray School of Medicine of Wake Forest University * * Medical Center Blvd * * Winston-Salem, NC 27157-1092 * * 910-716-4972 * * jjerome-at-isnet.is.wfu.edu * **************************************************************
On Wed, 9 Aug 1995, Shelly Landon in Spector Lab wrote:
} Hi! I am working with cell monolayers grown on collegen substrates attached } to thermanox coverslips. The resin is LRW. I mount them so I can cut the } Z axis. The question is has anyone cut thermanox, if so does this damage } the knife? The difficulty is in removing the thermanox to get to the cells } which is easily done with cell monolayers, but not collagen. Any ideas? } Shelley Landon Kaurin Landon-at-cshl.org } }
Just to set the tone for the upcoming MSA meeting, this true story:
A young boy asks the reference librarian who the curly headed little girl was who is in all the Shirley Temple movies.
The story is worth considering the next time you come up against a seemingly unsolvable microscope problem.
Jay Jerome ************************************************************** * aka: W. Gray Jerome * * Dept. of Pathology * * Bowman Gray School of Medicine of Wake Forest University * * Medical Center Blvd * * Winston-Salem, NC 27157-1092 * * 910-716-4972 * * jjerome-at-isnet.is.wfu.edu * **************************************************************
Comments: Converted from PROFS to RFC822 format by PUMP V2.2X Resent-Date: Thu, 10 Aug 95 00:05:49 EDT Resent-From: Glee Yorke {G0YORK01-at-ULKYVM.LOUISVILLE.EDU}
Anatomical Sciences & Neurobiol. Phone: 852-5178
----------------------------Original message---------------------------- Received: from ULKYVM by ULKYVM.LOUISVILLE.EDU (Mailer R2.10 ptf000) with BSMTP id 6752; Wed, 09 Aug 95 11:17:02 EDT Received: from biosci.mbp.missouri.edu by ULKYVM.LOUISVILLE.EDU (IBM VM SMTP V2R2) with TCP; Wed, 09 Aug 95 11:17:01 EDT Received: from [128.206.15.189] (phillips1.biosci.missouri.edu) by biosci.mbp.missouri.edu with SMTP (5.65c/IDA1.4.4.1-Domain/OS) id AA28553; Wed, 9 Aug 1995 10:14:09 -0500 Message-Id: {v01510103ac4e8fdb0233-at-[128.206.15.189]} Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
I posted a reply earlier and can't remember if I sent it to you directly or the Microscopy list. If I sent it to you directly, would you mind forwarding/posting it on the list as I am interested in getting the list feedback. thanks.
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility 3 Tucker Hall University of Missouri Columbia, MO 65211 (314)-882-4712 (voice) (314)-882-0123 (fax)
I recently received an Ad from Kodak concerning the RFS3570 film scanner it does a variety of film sizes from 35mm at 2100dpi to 70mm at 800dpi. Kodak also make a thing called PCD Imaging Workstation which contains a scanner (Kodak Professional Film Scanner 4045) which does film sizes up to 4x5 with 4kx6k pixels.
You can find out more from their web site:-www.kodak.com
MolecularCellSpeak List {molecular-cell-speak-at-mailbase.ac.uk} , Microscopy List {microscopy-at-aaem.amc.anl.gov} , Info-Bios List {info-bios-at-mom.spie.org} , Confocal Microscopy List {confocal%ubvm.BITNET-at-BITNIC.CREN.NET} , Cell Bio List {cellbiol-at-net.bio.net} , Biz-Biotech List {biz-biotech-at-netcom.com} , Bionews List {bionews-at-net.bio.net} , Biomaterial List {biomat-l-at-hearn}
Is Your Unique Cell Image Data going Unrecorded?
There is a Solution!
Digital Imaging of Your Live Cells.
Capture Important Structures Functions and Interactions of Your Live Cells In Situ!
Monitor and Document Your Live-Cell Experiments Routinely and Frequently.
Why Digital Imaging?
Digital Images can be: Displayed Manipulated Processed Stored Retrieved Shared Transported
All via your Personal Computer!
No need to invest in expensive system hardware and imaging personnel
Ease of Use Safe Archival Image Storage Economical Fast Turnaround Performed by Live-Cell Imaging Biologist Meticulous Care Taken with Your Live Cells Complete Confidentiality Assured
To image your live cells, contact:
Marc C. Brande, M.S. or Caroline Yu at Cell Applications, Inc. Toll-Free: 1-800-645-0848 Local: 619-453-0848 Fax: 619-453-2862 Email: BRANDE-at-SDSC.EDU
Marc C. Brande, M.S. SD3D Email List:3D Imaging San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu 3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830
**************************************************************************** Dr Ian MacLaren, IRC in Materials for High Peformance Applications, The University of Birmingham, Birmingham B15 2TT, England
Thanks very much to all who responded to my request regarding obtaining a used specimen holder for a Zeiss EM 9A TEM. There are a couple on the way to me. I appreciate the generosity of folk around the microscopy world - including offers of entire microscopes! Thanks too to Nestor for his work on this listserver. Carolyn Emerson, Dept. of Biology, Memorial Univ., St. John's Newfoundland Canada.
Dear Ian and colleagues, As a vendor, I had the same response you did to this particular posting that it crossed the invisible line between information and solicitation. Several of us (I am thinking particularly of Chuck Garber) have been very scrupulous about erring on the side of non-commercialism whenever there was any doubt in our minds about interpreting this rule. This sometimes involves exercising extreme restraint. I don't want to pick on this particular vendor, or start another prolonged harangue, but a little self-discipline goes a long way. Steven Slap, Vice-President, Energy Beam Sciences
D.J. Rawlins in "Light Microscopy" states "...buffers containing chloride ions (e.g. TrisHCl or phosphate buffered saline) should be avoided as there is the possibility that HCl vapor could be released which reacts with formaldehyde to give the very carcinogenic gas bis-chloromethylether."
I have never heard of this before. Is this conventional wisdom? What about fixes like Karnovsky's that contain CaCl2 to stabilize the membranes? What do people use to adjust the pH of their fixes? Is this a serious problem. I have been using HEPES-buffered saline fixes for quite a while. Any comments would be appreciated.
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility 3 Tucker Hall University of Missouri Columbia, MO 65211 (314)-882-4712 (voice) (314)-882-0123 (fax)
I am a beginner at this topic, so please bear with me.
My lab (government/marine research) is retrofitting a suite of rooms for a Zeiss TEM 900. I am the safety officer here and I have some concerns about chemical safety. WE have been advised that propylene oxide should be stored in an explosion-proof frig. Does everyone out there do that? Or will a lab-safe flammable frig suffice? Do they make small versions of the latter?
One more thing for now, must fixing and staining be performed under a hood, or local ventilation?
Thanks in advance, Annette Kubinec NOAA Howard Lab Ner Jersey 908-872-3011 akubinec-at-sh.nmfs.gov
} To: Annette S Kubinec {akubinec-at-sh.nmfs.gov} } From: gwe-at-biotech.ufl.edu (Greg Erdos) } Subject: Re: TEM prep room } Cc: } Bcc: } X-Attachments: } } } I am a beginner at this topic, so please bear with me. } } } } My lab (government/marine research) is retrofitting a suite } } of rooms for a Zeiss TEM 900. I am the safety officer here } } and I have some concerns about chemical safety. WE have been advised } } that propylene oxide should be stored in an explosion-proof frig. } } Does everyone out there do that? Or will a lab-safe flammable } } frig suffice? Do they make small versions of the latter? } } } } One more thing for now, must fixing and staining be performed } } under a hood, or local ventilation? } } } } Thanks in advance, } } Annette Kubinec } } NOAA Howard Lab } } Ner Jersey } } 908-872-3011 } } akubinec-at-sh.nmfs.gov } } } -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- } } We have discontinued the use of propylene oxide in our protocols, so we avoid that problem. Acetone works just as well as a transitional solvent. } } Certainly osmium must be used in a fume hood and I would recommend that all aldehydes be used there as much as possible. Sometimes it is necessary to do some aldehyde work out side the hood but keep the conainers closed as much as possible} } } It is probably aslo a good idea to use open embedding chemicals in the fume hood too, again keeping them closed as much as possible when they are outside the hood. } } Staining with aqueous stains can be done safely outside the hood but heavy metal wastes should be properly disposed of. } } There is an Electron Microscopy Safety Handbook by Barber & Moscorro published by San Francisco Press, Inc., Box 426800, CA 94142-6800. } } This would be a good good thing for any safety officers to have if an EM Lab is part of their domain, and another copy in the lab as well. } -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- -at- Greg Erdos Phone: 904-392-1295 -at- -at- Scientific Director, -at- -at- ICBR Electron Microscopy Core Lab -at- -at- 218 Carr Hall Fax 904-846-0251 -at- -at- University of Florida E-mail: gwe-at-biotech.ufl.edu -at- -at- Gainesville, FL 32611 -at- -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
I disagree with not wishing to make a fuss here, this is truely blatent advertising, and absolutly should not be tolerated on this listserver. Let me make clear that I very much enjoy and hartily encourage vendor participation on this listserver - but the key word is participation, as in answering or making commentary on specific issues and questions brought up via the group.
However blatent, unsolicited advertising shall not be tollerated. I have to deal with these ad's on the news groups enough already. And we as a group can take the same modes of action as are taken on the news groups. (1) flame the offenders, (2) contact their postmaster and clearly state their offense, and (3) if repeated seek termination of their e-mail access via their postmaster/sysop.
In this case this is not a simple error, the extended list of CC's shows that this was intended to be a blatent AD. Further more the list of rules which every subscriber of this group received when they subscribe clearly state no advestisment.
I apologize to the group for this rant but I truely felt it had to be said.
A Zeiss EM 109 and a Zeiss TEM 10 are available. Both are in excellent shape. The price is negotiable as the seller is motivated! If you are interested, you can contact Karl at 908-370-8082.
In a recent cleanup of the lab we discovered a Sorvall TC-2 Tissue Sectionner that hasn't been used in over 10 yrs. Someone in the lab would like to use it, however, we can't find a manual. Also need to find a supply of new blades. Who supplies Sorvall equipment now adays?? Is the Tissue Sectionner still being made. Does someone have unused blades they no longer require?? Any help would be greatly appreciated.
Thanks
Mark Elliott UBC-Pulmonary Research Lab, Vancouver BC
-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --
For list subscribers who will be at the MSA meeting we offer this free service to make it easier to "stay in touch" when away:
We will have in our exhibit booth a FAX machine during the hours of the exhibition. Subscribers to the "list" can send or receive FAXed (short) messages to or from anywhere in the world. Just announce to our booth staff that you are from the "listserver".
To receive a FAX, give out the following number: 1- (816) 871-3421. Tell the sender to dial the number and then IMMEDIATELY press the "go" button so we "see" it as an incoming FAX and not a phone call. Stop by as often as you like to see if anything has come for you.
We will also be on line much of the time with our web site for those who do not yet have "web access" and who want to learn how to gain access. So if the line is busy, be patient and try again.
Yes, we do have an ulterior motive. We would like to meet as many of our colleagues on the list server as possible. I believe such eye-ball to eye-ball contact enhances the quality of communications in a world more and more defined by computer screens and keyboards.
Chuck
====================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: GVKM07A-at-prodigy.com West Chester, PA 19381-0656 USA Customer Service: SpiSupp-at-aol.com
Does anyone know of a specfic reference for doing EM immunocytochemistry on yeast cells? I am trying to localize nuclear pore proteins in yeast using the whole yeast cell not just a nuclear membrane prep. I have tried preembed IMC with Fluoronanagold (1.4nm) with silver enhancement, but did not get great results. Any suggestions would be a great help.
Sincerely,
Christine A. Snay Dartmouth Medical School Hanover, NH 03755 e-mail Christine A. Snay-at-dartmouth.edu 603-650-1905
In our Surg Path Lab, decalcified tissue is routinely washed before transfer to formalin. We use RDO Rapid Decalcifier which is concentrated HCl (from Apex Engineering Products Corp).
The container states "simultaneous formaldehyde fixation and decalcification with RDO should not be performed. A potential carcinogen could result from this mixture."
The Reactivity Data in the MSDS indicates that hydrogen chloride and formaldehyde gas react to form bischloromethyl ether, a carcinogenic compound.
I have always used HCl to adjust the pH of EM fixatives-never made the connection between Decal(HCl)/formaldehyde incompatibility and use of HCl when pH'ing EM fixatives.
Can a few drops of dilute HCl in formaldehyde/paraformaldehyde produce bischloromethyl ether?
Once again you mere mortals have been sending unsubscribe messages-
As scientists you should have figured out by now that unsubscribing is not allowed. As we have pointed out in the past, once you subscribe you are on the list for "life" - whichever life comes first, yours or ours. Furthermore, should you change your e-mail address, we will track you down and make sure that the list messages get to you.
Have you ever noticed that even if you change phone numbers, the people who sell aluminum siding, ginzu knives, Kirby vacuum cleaners, Sears Appliance Service Contracts, and such always seem to find you no matter what? That is because their computers, like this listserver computer, are equipped with special software from
Boston American Resource Finders.
BARF software uses proprietary routines to track you down wherever you are.
The only way to contradict BARF algorithms is to say pretty please and send your unsubscription request to the correct address:
DISCLAIMER: The characters portrayed in this message are not real and their names were changed to protect the innocent. Which of course begs the question "Is Nestor real and is he innocent?"
Jay Jerome ************************************************************** * aka: W. Gray Jerome * * Dept. of Pathology * * Bowman Gray School of Medicine of Wake Forest University * * Medical Center Blvd * * Winston-Salem, NC 27157-1092 * * 910-716-4972 * * jjerome-at-isnet.is.wfu.edu * **************************************************************
--Boundary (ID v3WOuY0YgDrNRNY2cptueA) Content-type: TEXT/PLAIN
As someone who is shopping around for a system with which to collect digital images from my TEM, I've been reading the recent film scanner postings with the following questions in mind:
"If you can digitize directly, why would a film scanner be necessary?" and "Don't you end up with some HUGE whopping files to store if you are scanning at that type of resolution?"
My intention is to replace the cost and long turnaround time between viewing and reporting that I have using standard plates with an "instant", low cost harcopy solution (likely a CCD / 35mm port interface, thermal prints at the scope, dry silver for reports). Only diff pats and the occaisional lattice image would be recorded on film.
The direct digital images I've seen appear to be of sufficient quality for the majority of standard microstructural images collected here. So the big question is, do I need a film scanner as well?
} Dear Colleagues, } } Does anyone know of a specfic reference for doing EM immunocytochemistry on } yeast cells? I am trying to localize nuclear pore proteins in yeast using the } whole yeast cell not just a nuclear membrane prep. I have tried preembed IMC } with Fluoronanagold (1.4nm) with silver enhancement, but did not get great } results. Any suggestions would be a great help. } } Sincerely, } } Christine A. Snay } Dartmouth Medical School } Hanover, NH 03755 } e-mail } Christine A. Snay-at-dartmouth.edu } 603-650-1905 } -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
You might try Anderson, etal., J. Electron Microsocpy Technique 18:172-175, 1991}
Or
Clark, 1991, Meth. in Enzymology 194: 608-262. -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- -at- Greg Erdos Phone: 904-392-1295 -at- -at- Scientific Director, -at- -at- ICBR Electron Microscopy Core Lab -at- -at- 218 Carr Hall Fax 904-846-0251 -at- -at- University of Florida E-mail: gwe-at-biotech.ufl.edu -at- -at- Gainesville, FL 32611 -at- -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
The Electron Microscopy Safety Handbook, Vernon C. Barber and Deborah L. Clayton, San Francisco Press, Box 6800, San Francisco, CA 94101-6800, ISBN 0-911302-56-5, LC # 85-051796 would be a valuable reference for you. I do all staining, fixation, and embedding under a hood, and have my embedding ovens under a hood. Unopened bottles of propylene oxide are kept in the refrigerator and opened bottles under the hood. Before I came to this lab, an explosion had occurred in the refrigerator as an opened bottle of prop ox had been in the refrigerator, fumes had accumulated, and evidently explosion occurred. I worked in n lab without air conditioning at one time and all the lids popped off the vials. On Thu, 10 Aug 1995, Annette S Kubinec wrote:
} I am a beginner at this topic, so please bear with me. } } My lab (government/marine research) is retrofitting a suite } of rooms for a Zeiss TEM 900. I am the safety officer here } and I have some concerns about chemical safety. WE have been advised } that propylene oxide should be stored in an explosion-proof frig. } Does everyone out there do that? Or will a lab-safe flammable } frig suffice? Do they make small versions of the latter? } } One more thing for now, must fixing and staining be performed } under a hood, or local ventilation? } } Thanks in advance, } Annette Kubinec } NOAA Howard Lab } Ner Jersey } 908-872-3011 } akubinec-at-sh.nmfs.gov }
Here at MCO we do have safety in mind, all of our tissue processing is performed in a fume hood. The resin (Spurr's) is polymerized in an oven which is vented to an exhust fan. All of our Flammable Chemicals are in a flammable cabinet which is also vented to an exhust fan. Our refrig is a type which was made for storage of flammable materials. The best advice is not to have a lot of flammable materials in the lab, this means good inventory/ordering on your part. A few other safety items: Have in the lab at readily accessable points the following; fire extinguisher, first aid kit, chemical spill kits, eye wash stations,(shower), soap and towels, biohazard bags, sharps containers,lots of warning signs, MSDS's, and the list goes on and on. The "safer" you can make a lab the begin with the better. One other item, pay particular attention to the work flow/space. You do not want areas where people will be bumping into each other - SPACE - is the key word here.
Best of Luck,
Ed Calomeni Medical College of Ohio Toledo, OH emlab-at-opus.mco.edu
The best source for parts and advice regarding the old Sorvall tissue chopper is a former Sorvall sales/service expert named Bill McGee. Bill's company is called Microtome Services Co, 7568 Florian Way, Liverpool, NY 13088. His phone number is (315)451-1404 (no fax or e-mail, sorry). Tell him Steve sent you. Steven Slap
the Kodak is 1000 x 1000 lines of resolution greyscale. we use it for electron microscopy, and it is adequate. The JVC will capture up to 3500 x 3000 lines, color 16 million. The JVC requires an immobile subject. we use it for light microscopy, and the screen images are astounding.
There are many advantages to video photography, but, before you buy, consider: price image storage (jpg format may not be acceptable for legal purposes) printing
I am writing an article on this subject, and I actually built the JVC system from parts. If you know of a journal which would be interested in publishing my findings, please tell me.
I am looking for a plate or plates that show electron micrographs (sections and neg stains) of the major virus families. G.D. Hsiung published such a plate several years ago but I can not locate her. Does anyone have such information or know where I could locate Dr. Hsiung (e-mail, phone, etc).
Also, anyone know where to locate actual micrographic prints (not digital images) of alpha- flaviviruses, Ebola, etc. We would like to include them in a textbook we are revising.
Thank you all very much.
Peace.
############################################################################# John J. Bozzola, Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901-4402 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html #############################################################################
I am going to be teaching two courses next year: a) Introduction to Materials Science b) Introduction to Electron Microscopy
I am thinking about using Web Pages for various material, at the most obvious level homework (and answers). Has anyone had experience doing this in the past, any suggestions (or horror stories), and is there anyone who has material which might be useful ?
As a part of our opening at MSA'95, news from the meeting will be posted in our What's New column. If you would like to participate, please stop by booth 464 and we will post the information on the Internet during the show. If you can't make the show, I will be checking for FAXES at the SPI booth.
Susanne Pignolet Brandom, Ph.D. spb-at-wwa.com or spignole-at-ix.netcom.com 708-548-6522
MicroWorld Resources and News http://mwrn.ms.wwa.com/topfile.htm
gbs-at-ariadne.phys.uts.edu.au (Geoff Smith), Microscopy-at-aaem.amc.anl.gov (Microscopy List) In-Reply-To: {199508121709.AA04206-at-apollo.numis.nwu.edu} from "L.D.Marks" at Aug 12, 95 12:09:55 pm X-Mailer: ELM [version 2.4 PL23] Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Content-Length: 2607
L.D.Marks posted an enquiry about the use of the WEB for teaching. There are some general issues of "teachnology" which may be of general interest so I have posted this response to the list.(apologies to those who have "research only" jobs!)
I have been developing mixed feelings about the extent to which the Web SHOULD be used as a teaching tool: * A complete replacement for the human being it should never be. * The electronic library character (+speed/convenience) is a bonus for students * However, to make a teaching tool most beneficial it needs to be genuinely designed for student learning, not just messing about and picking up info here & there.
I use a Subject homepage as a electronic bulletin board and summary sheet for my students.
I am currently developing an interactive "webbed" version of a computer simulation tool for understanding magnetic properties of materials. (unfortunately I don't get to teach much microscopy at the moment)
Perhaps something else to consider is the use of an email newsgroup for students, it keeps them in contact with you and each other. (not unlike this Listserver !!)
I'd welcome feedback/discussion on this issue.
} I am going to be teaching two courses next year: } a) Introduction to Materials Science } b) Introduction to Electron Microscopy } I am thinking about using Web Pages for various material, at the } most obvious level homework (and answers). Has anyone had experience } doing this in the past, any suggestions (or horror stories), and is } there anyone who has material which might be useful ?
I think what Dr Marks is proposing is about right and not too difficult to achieve provided: * your students are ALL computer and net confident and have easy access to the WWW * you are happy for your material to be available to "the world" (there are ways around this) * you build in good feedback into the electronic homework sheets so students aren't just left knowing they're are wrong
PS: Who's coming to beautiful Sydney next February ?????
Kind regards,
Dr David Green Lecturer in Applied Physics University of Technology, Sydney P.O. Box 123 TELEPHONE: + 61 2 330 2203 Broadway 2007 NSW A MESSAGE: + 61 2 330 2206 Australia FACSIMILE: + 61 2 330 2219 EMAIL: dcg-at-phys.uts.edu.au WEB: http://www.phys.uts.edu.au/~dcg/DavidGreen.html or http://www.uts.edu.au/ if you like "surfing" ------------------------------------------------------------------ Watch out on the information superhighway... crazy driver with "P" plates. ------------------------------------------------------------------
The recent exchange of messages regarding what is acceptable for commercial vendor postings on this list server has prompted me to remind you that Microscopy Online offers a forum for posting commercial information that non-commercial users will find entirely acceptable. Microscopy Online is a server on the WWW that allows everyone (commercial or not) access to post information (text, graphics) that is related to microscopy, whether you have access to the WWW or not. The URL is
I forbade the use of propylene oxide in my lab 25 years ago. It is too much of a fire/explosion risk. Look at the flashpoint temperature on the bottle! minus 29 deg. celsius! Even petrol is better at minus 18 deg. C.
Luft suggested its use as a transitional solvent because he argued the epoxy group meant any solvent carried over into the embedment would be incorporated into the molecular structure of the epoxy resin. But the boiling point is 35 deg. C. As soon as an embedment is put into a curing oven at 60 deg. any epoxy propane in the resin will flash into vapour and lucky it doens't blow the door off! I conclude the only benefit of epoxy propane is its miscibility and low boiling point. We use acetone, which boils around 55 deg. It is only about as dangerous as petrol and will vaporise easily at 60 deg. in the curing oven. Ethanol, boiling at 78 deg. will not, and resin containing carried over ethanol stays sticky.
} "If you can digitize directly, why would a film scanner be necessary?"
Once you start working with digital files you won't want to go back to your darkroom. THEN you need some way to digitise the plates/films you have archived over the years.
} and } "Don't you end up with some HUGE whopping files to store if you are } scanning at that type of resolution?"
Scan at a resolution thats acceptable. Say 1024x1024. You can store 500 such images on one CD-ROM at a cost of $18 per disc. Films are about $2 a shot including processing.
X-Mailer: WinNET Mail, v2.30 Message-ID: {776-at-oimag.win-uk.net} Reply-To: Software department {software-at-oimag.win-uk.net} To: USERHHXS-at-um.cc.umich.edu, microscopy-at-aaem.amc.anl.gov
Obviously, the effect depends on the system you have and the precision with which you want to acquire spectra.
When power is applied to the detector, there are various time constants, both electronic and thermal, which affect how long the system takes to reach a stable operating point. Most electronic circuits generate heat and gain and offsets are temperature sensitive which is why the thermal time constant is relevant. If the detecting crystal has been exposed to a high radiation dose, removing and restoring the HT can affect the behaviour, but there are no rules and not all systems perform the same way.
An easy way to check this is to record a series of spectra sequentially, immediately after the system is switched on from cold. Whereas gross changes will be apparent in the first few minutes, the spectra will probably seem to settle down after half an hour typically. However, if you want to do accurate spectrum processing and expect the software to sort out severe peak overlaps, accurate peak position and width is essential and you will need a more sensitive method to pick up the changes.
If you don't have any peak fitting diagnostic software to hand, one thing you can do is simply subtract one spectrum from the next one acquired and view the difference spectrum. Provided the two spectra have reasonable statistics, the difference spectrum will show up minute differences in gain, offset or resolution, in any region where there is a major peak. A shift of only 1eV between spectra will typically produce a bipolar residual of very roughly 1% of the peak height. Of course, you will have to make sure your microscope has good beam current stability to do these tests. If not, you will have to normalise each spectrum to the same area before subtraction.
Peter Statham Oxford Instruments Microanalysis Group
} I am interested in opinions concerning the relative merits } of keeping an EDS HV bias on continiously, as opposed to } shutting it off after every use. In other words, is there } any advantage wrt to stability of the electronics or } energy calibration to keeping it on? }
----------------------------------------------------------------- Please reply to this e-mail with the name of the person you wish to receive it on the subject line (e.g. "FAO Janet Smythe/..subject.."), as this is a shared e-mail address.
Several days ago I put one message about the loss of information (MAG. & scale-bar) on negatives in JEOL 2010F microscope. Thanks to all the people who gave me kind correspondences.
Nowadays, we know why we lost the information. It is because we use the FLC, i.e. free lens control, function. Even though I only changed the current of the condenser lens and did not change the magnification, the CRT under FLC will in no way give you information about MAG. on negatives. That is not the problem of the microscope, but what we should learn. It is my pleasure to post the "answer" here for your interest.
I have seen discussion of several of the various storage formats for archiving electronic images--i.e. read/write CD, magneto optical, tapes, etc. What are the collective thoughts on 3.5" cartridges (such as Syquest)? They can hold up to 270 Mb and would seem to be a cheap (~$65) alternative that can be read by any machine with a 3.5" drive.
Bob
Robert R. Wise, PhD Director, UWO Electron Microscope Facility Department of Biology University of Wisconsin Oshkosh Oshkosh, WI 54901 (414) 424-3404 (414) 424-1101 fax wise-at-vaxa.cis.uwosh.edu
Less for archiving and more for convenience sake, although this works for archiving, we have 230 MB read/write magneto optical disk drives on each of our Macintoshes and lots of other people around the school have them too (either Olympus or Fujitsu mechanism). The upsides are that the disks are cheap ($40), small, hold a lot, are random access, each user is responsible for his/her own data and having a drive on each machine eliminates the need for any type of network support. The downside is that they are slower than some other media choices. For instance, for moving large amounts of data from hard disk to archive and back, a 4mm DAT drive is faster, but attempting to use this in a multi-user faciility just didn't work. -Michael Cammer
On Mon, 14 Aug 1995 wise-at-vaxa.cis.uwosh.edu wrote:
} To all, } } I have seen discussion of several of the various storage formats } for archiving electronic images--i.e. read/write CD, magneto optical, } tapes, etc. What are the collective thoughts on 3.5" cartridges (such as } Syquest)? They can hold up to 270 Mb and would seem to be a cheap (~$65) } alternative that can be read by any machine with a 3.5" drive. } } Bob } } } Robert R. Wise, PhD } Director, UWO Electron Microscope Facility } Department of Biology } University of Wisconsin Oshkosh } Oshkosh, WI 54901 } (414) 424-3404 } (414) 424-1101 fax } wise-at-vaxa.cis.uwosh.edu } } }
$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$ $$$$$$$$$$$$$$$$$$$$$$$$$$$ $$$$$$$ from laughter or from fright.$$$ $$$an ether-eating eskimo $$$$$$$$$$$$$ convulsed into oblivion $$$$$$$$$$$$$$ $$$$$$$$$$$$$$$$$$$$ would gag upon your sight, $$$$$$$$$$$$$$$$$ $ ---Residents $$$$$$$$$$$$$$$$$ $$$$$$$$$$$$$$$$$$
Comments: Authenticated sender is {wesleysm-at-biology.und.ac.za}
------- Forwarded Message Follows -------
Dear colleagues Thanks to those of you who came forward with comments regarding the merits of using cacodylate buffer in specimen preparation for microscopy.
Replies could be summarised as follows: 1. The use of cacodylate buffer is recommended for enzyme cytochemistry work for the following reasons: a. The use of Sorensens phosphate buffer can be damaging to mitochondrial activity. b. The use of phosphate buffers is incompatible with lead precipitation reactions. c. Cacodylate buffers will not react with aldehyde fixatives, as other amine-containing buffers will (e.g. TRIS).
2. It seems that the use of cacodylate by 'tradition' or because users follow " a paper I read some years ago..." is not unique to this lab. Common sense dictates for a pilot study to be done at the beginning of a project to assess whether the results from using this buffer are, in fact, superior to alternative ones. Unfortunately, this is usually ignored in the interset of expediency, and the "proven" method wins.
3. Perhaps the most surprising piece of information was that cacodylate buffer CAN support microorganisms, as is the case with demethylating bacteria. (Another theory bites the dust!).
Everyone agrees on its toxicity, the need to use it for specialised applications only, and to collect residues for its disposal. (We collect it in a 2.5l bottle in the fume-hood, and it is removed by a waste disposal firm. Does anyone known what they do with it?)
This has been an interesting exercise for me, and one which makes this medium an invaluable forum to exchange ideas.
Thank you.
James Wesley-Smith Electron Microscope Unit George Campbell Building University of Natal Durban, South Africa
Message-ID: {1995Aug15.070220.254693-at-mailstop.pharmetr.com} To: microscopy-at-aaem.amc.anl.gov
MUST SELL
A Zeiss 10CA transmission electron microscope in top condition with Coolwell closed-loop cooling system, recently overhauled rotary and diffusion pumps, two 3 1/4 x 4" negative cassette carriers with 90 hegative cassettes. The microscope maintains an excellent vacuum, a point to point resolution of 3 angstroms, and accelerating voltages of 20-40-60-80- and 100kV. The microscope comes with all of the necessary accessories, including extra filaments. The asking price is $35,000, however the best offer will be accepted.
Also available: a complete darkroom setup, virtually brand new, including fully equipped stainless steel sink (24x72x5"), developing tanks and water jacket processor, print washer, RC print dryer, negative drying oven, Durst L1200 enlarger with condensors, lens and carrier, and sodium arc safelight. In addition: a Leica cryocut 1800 cryostat, a Sorvall MT-2B ultramicrotome, and LKB 7800 glass knifebreaker. All of these items may be sold as a package or separately. Please E-mail or call for more information and pricing: Steve Grayson: Tel# (415) 617-9327 or FAX (415) 853-6301 *************************************************************************** Pharmetrix, 1330 O'Brien Drive, Menlo Park, CA, 94025 Main: (415)688-0100 Fax: (415)688-0115 The views expressed in this posting those of the individual author only. ***************************************************************************
Does anyone out there have the secret to consistently producing good glass knives on an LKB 7800 knifemaker? I have cleaned, adjusted, and fiddled with it for two years and can get still get only average knives, and those only some of the time. Students new to the instrument almost never get good results. I contacted LKB and they sent a couple of brochures that seemed to be only marginally helpful. Is it in the black magic associated with cleaning the glass, brand of glass (we use Ted Pella glass), or the phase of the moon?
Bob
Robert R. Wise, PhD Director, UWO Electron Microscope Facility Department of Biology University of Wisconsin Oshkosh Oshkosh, WI 54901 (414) 424-3404 (414) 424-1101 fax wise-at-vaxa.cis.uwosh.edu
Depending on the number of grids you stain per day, this little instrument might be of use to you. It is the Hiraoka Staining Dish which will stain up to 40 grids at one time. We purchased ours through Probing & Structure (Townsville, Qld. Australia) or you can get it through Electron Microscopy Sciences. I use it all the time and find it very reliable and easy to use.
Regards, Gerald.
Dr. Gerald J. Little, The Neuroscience Group, Discipline of Anatomy, Faculty of Medicine and Health Sciences, The University of Newcastle, Callaghan, New South Wales, Australia, 2308. Ph. (61 49) 21 5618 Fax (61 49) 21 6903 Email ANGJL-at-Medicine.Newcastle.edu.au
I believe our lab. once tried another brand of glass after always using LKB glass and quickly returned to the more expensive LKB brand. However that was quite a few years ago, we brought enough glass to last for ever...!
Digital Image Restoration Microscopy, or Deconvolution Microscopy... I am looking for any information, including contact email or fax nos of the manufacturers. Thanks, Sally . ---------------------------------------------------------------------- Sally Stowe Australian National Univ. Facility Coordinator Canberra, AUSTRALIA ANU Electron Microscopy Unit Ph 61 6 249 2743 RSBS, Box 475 Email stowe-at-rsbs-central.anu.edu.au FAX 61 6 249 4891 ------------------------------------- -------------------------------- -
Bob Wise, University of Wisconsin seems to be following a track that most owners of LKB 7800's seem to have at one time or another. But there is a solution at hand Bob. First let me reassure you;
1) Phase of the moon is important, and, 2) Not all microtomy glass is equal
We are fortunate to have a gifted engineer in one of our workshops and he has 'breathed' on our 7800 to remarkable effect. Our target was between 6 and 8 out of 10 good knives. Jan Slot, when he visited our laboratory, said that he had never met anyone who had investigated the working of a knifemaker as thoroughly. But enough waffle - we published the mods;
Journal of Microscopy, Vol.168, Pt 1, October 1992, pp111-114. 'Further modification of the LKB 7800 series Knifemaker for improved reproducibility in breaking 'cryo' knives'
Stay in touch , there have been further refinements - but modify your unit according to the detailed instructions given in the paper first.
Tony Bruton Electron Microscope Unit University of Natal Pietermaritzburg Kwazulu Natal; South Africa Tel: (0331) 2605155, Fax : (0331)2605776, email : BRUTON-at-EMU.UNP.AC.ZA
We would like to build up a library of training videos on aspects of microscopy. Does anyone know any good ones? I would also appreciate information on their current availability.
Bob Wise, University of Wisconsin seems to be following a track that most owners of LKB 7800's seem to have at one time or another. But there is a solution at hand Bob. First let me reassure you;
1) Phase of the moon is important, and, 2) Not all microtomy glass is equal
We are fortunate to have a gifted engineer in one of our workshops and he has 'breathed' on our 7800 to remarkable effect. Our target was between 6 and 8 out of 10 good knives. Jan Slot, when he visited our laboratory, said that he had never met anyone who had investigated the working of a knifemaker as thoroughly. But enough waffle - we published the mods;
Journal of Microscopy, Vol.168, Pt 1, October 1992, pp111-114. 'Further modification of the LKB 7800 series Knifemaker for improved reproducibility in breaking 'cryo' knives'
Stay in touch , there have been further refinements - but modify your unit according to the detailed instructions given in the paper first.
Tony Bruton Electron Microscope Unit University of Natal Pietermaritzburg Kwazulu Natal; South Africa Tel: (0331) 2605155, Fax : (0331)2605776, email : BRUTON-at-EMU.UNP.AC.ZA
The old LKB glass is available from Ted Pella, and it really makes a difference. Has your knifemaker had a good tuneup lately (scoring wheel, clean slides of the head, caliper-measurement of the squares, fresh dampening pad)?? Careful attention to all of the above can make a huge difference in knife quality. I recently put in a nice fresh scoring wheel and carefully tweaked the scoring pressure with a great increase in knife yield (and consistency). Grace Kennedy PS Caliper-measure your squares!!
Introducing the workshop: IMAGING CELLULAR DYNAMICS DURING DEVELOPMENT & REPRODUCTION and its accompanying symposium: EX OVO OMNIA: FROM THE EGG, EVERYTHING!
Dates: Workshop - January 7-24, 1996 Symposium - January 21-24, 1996
Place: University of the Witwatersrand Johannesburg, South Africa
The workshop course is designed to familiarize young scientists with modern approaches for exploring structural events at the cell and molecular level. The course will rely heavily on invertebrate and vertebrate eggs and embryos to explore dynamic events in the cell cycle and during development. Participants will use advanced imaging technologies through full time laboratory work, and be exposed to advanced topics in Cell Biology, Developmental Biology, and Reproductive Biology during daily lectures. Twenty-four young scientists will be accepted, with a preference for doctoral level (or equivalent) back-ground, who are in the beginning of their scientific careers.
To apply for the workshop, please mail, fax or e-mail no later than September 15: 1. Name, address, fax, email, and academic affiliation. 2. Curriculum vita. 3. Three letters of recommendation. 4. A proposal which states: (A) the reason for applying. (B) how the course will benefit you and your local community both in terms of scientific research, as well as scientific instruction. (C) proof of complementary financial support from your institution, as well as anticipated travel expenses.
To: Professor Barry Fabian Professor Gerald Schatten Univ. Of the Witwatersrand Univ. Of Wisconsin Private Bag 3, WITS 1117 W. Johnson Johannesburg, 2050 S. Africa Madison, WI 53706 USA Fax: 27.11.339.3407 Fax: 608.262.7319 Email: Barry-at-gecko.biol.wits.ac.za Email:Schatten-at-macc.wisc.edu
A special International Symposium on Developmental Biology has been arranged to coincide with the conclusion of the laboratory training course. The theme of the symposium addresses development at the molecular, cellular and organismic levels, with presentations in the form of talks and posters. Contact Barry Fabian or Gerald Schatten for information about poster presentation and registration.
Amy Haavisto Schatten Lab University of Wisconsin - Madison Haavisto-at-students.wisc.edu Tel: 608-262-2048 Fax: 608-262-7319
I found the Preparation for Millonig's buffer in Hayat's Electron Microscopy. Surprise, it isn't in Manual of Electron Microscopy by G.Millonig (1974).
The preparation is Millonig's Buffer (1964)(Taken from Hayat, M.A., 1989, Electron Microscopy, 3rd ed., pg 23)
} Would someone please e-mail me the protocol for mixing up Millonig's } phosphate buffer (I believe it was developed in 1964). } } Thanks, } } Ron } lherault-at-acs.bu.edu } later dlb
Thanks for all the response to my query on LKB 7800 knife makers. I got plenty of tips to try and sources to look into. I share the accumulated wisdom with the net (even though some of it you have already seen). I have three weeks before the semester and my EM techniques class start to get our KnifeMaker tuned up. I'll let you know how it performs in the hands of the ulitmate consumers (students).
Bob
****************
Comments ( in no particular order):
---For resin embedded sections, the solution might be to make a softer resin mix and cut thicker sections. ---If you want really sharp glass knives for cutting cryosections then check out J. Microsc. (1993 169:85-88) where I describe a simple way to get very good glass knives with only minimal modification. ---Another way to increase your chances of getting a good edge is to use 10 mm thick glass, which is hard to break but even really bad glass knives have a good length of useable knife edge in the middle. ---We have had problems with glass from other suppliers so stick to the strips made in Sweden (we get ours from EMS). ---The best knifemakers were the really old ones with a light blue case. The engineering on them is so much better than on the new ones. ---Try using LKB glass. It is slightly different in thickness than any other glass manufactured. ---Could you define what a good knife is (% of usable defect-free edge)? I consider 25-30% good edge normal and 50% a lucky break. It is not at all unusual to have half the knives made rejected upon inspection. ---Perhaps the scoring wheel is worn. Remove it and inspect under a dissecting microscope. Look at the cutting edge; if the surface looks like an upside-down "V" (/\) it's OK. If that apex has a flat rim /-\ then the score may not be enough to define the break initiation zone. ---Pella glass is fine, if you can find some LKB glass it would be worth trying. ---If you need a scoring wheel, try Leica. They absorbed LKB, Cambridge, A/O and Reichert recently. --- A slow tensioning of the pressure is the recommended procedure but you probably already knew that. ---Your problem might be the resin hardness. We never managed to cut sections using the resin formula we use with diamond knives, it only worked with a softer resin and thicker sections. ---You might also check out Stang 1988 J. Microsc. 149:77-79 for a really serious modification. ---Another interesting paper is Roberts 1975 J. Microsc.103: 113-119. This is a description of how tungsten coating glass knives can inprove their sectioning properties and shelf life. However, I can tell you that this does not improve a poor quality knife and too much tungsten can ruin a good knife. ---There is a short chapter in "Griffiths, G. 1993. Fine Structure Immunocytochemistry. Springer Verlag" where glass knives and tungsten coating are discussed. ---The scoring wheel needs to be sharp. ---Does the geometry all work? Is the line that bisects the square in the right place? ---Most of it lies in the glass. Is the long pre-fractured face of the strip at right angles to the top/bottom face? Is it smooth? Is it clean? Does the glass break readily with a small snap? Poor glass takes a lot of force and breaks with a loud BONK. Bad because too much energy is dissipated in the break. ---The clamping head that holds the scorer must slide up and down freely. Light oil on the slide helps. Note the lever only clamps the movable slide to the slideway. For the downward pressure to be the same each time friction in the slide should be minimal so the mass of the scorer always exerts the same force. ---To encourage uniformity, we use a "jiggle" with each clamping action. Jiggle (the clamp!) when clamping the square to make the diagonal score. Jiggle the scorer when lowering it onto the square to encourage good and even contact. ---I don't know about the LKB 7800 knifemaker especifically, but I have been make good knives in a Reichert knifemaker (for crioultramicrotomy). Some basic aspects are very important in my opinion: - Uniformity in the knife breakdown ---} Breakdown velocity (low = uniform = best edge). ---} Counter piece size (usually lower than 2 mm) /| / | / | / | counter piece-} |____|
---} knife angle - Usually 45o. if you need a "fine" edge, you can use a smaller one (but less resistant edge) for sectionning of "hard" specimens, the opposite.
*very important* ---} How to select the best knife?
Eye
/ | / | /____| Knife
\|/
Light source
If you see the knife edge like a very fine (invisible? :)) line, your knife is OK. You will see the edge like a bright line over a dark field, in this system. 10 - 20% of EXCELLENT knives is a very good mark.
---Pay attention if the cutting (tungsten) disk is ok. ---Phase of the moon is important ---Not all microtomy glass is equal ---We are fortunate to have a gifted engineer in one of our workshops and he has 'breathed' on our 7800 to remarkable effect. Our target was between 6 and 8 out of 10 good knives. Jan Slot, when he visited our laboratory, said that he had never met anyone who had investigated the working of a knifemaker as thoroughly. We published the mods; Journal of Microscopy, Vol.168, Pt 1, October 1992, pp111-114. 'Further modification of the LKB 7800 series Knifemaker for improved reproducibility in breaking 'cryo' knives' ---The LKB knife maker should be able to be adjusted to produce excellent glass knives. Leica (Deerfield, IL) should be able to give you some kind of assistance (what is left of LKB is owned by Leica). ---With regard to the knife glass, the LKB glass for many years the only reliable source for really outstanding glass. Because of the very high price, some other firms from time to time, came along offering glass of inferior characteristics. ---Today, in addition to the glass that you could get from LKB, others are offering glass that to the best that I can determine, would be indistinguishable relative to the "standard" LKB glass. The glass that is offered by Ted Pella and SPI are also found to be indistinguishable from the LKB product. However, not all of the glass being represented as being "good" for glass knives for thin sections comes from the same place. Some people think there is only one source but that is just not true. ---The sharpness of the edges is the critical parameter in terms of determining glass quality. That is why the glass comes so carefully wrapped. ---Glass is very heavy and a very high percentage of the price you pay is strictly related to transportation costs. ---The Messer Knife maker (Japan--imported by SPI) uses ordinary "float" glass, either 5 mm or 6 mm thick, the kind that you can buy at your local glass shop. It is very cheap to operate. ---We use an LKB 78018 knife maker, which I believe is the same idea as the 7800. After initial getting used to the thing I have had no problems at all producing excellent knives (so much so that I often prefer them to diamond knives, but for their durability of course). Just a few hints which you probably know already: a) make sure the diamond cutter is not wobbly, b) ensure that the holding block sits firmly on the glass and is secured tightly, c) use only 'well matured' glass; the older the better (we are using stock from the sixties supplied by LKB at the time), d) break in one movement, without jerks, e) take care with the little rubber buffer, it somtimes pushes the knives-to-be in too far, f) good luck (a very scientific precondition to success...). ---No glass chips on the feet (obvious). ---No old lubricant binding up the clamping head as it drops. It should slide down easily enough to clunk solidly on the strip if one is careless with the handle. ---Clean, *sharp*, *freely-rotating* cutter wheel. If this is bad, simply replacing it can make all the difference ---*NO* rotational play in the cutting slide. That's taken out by a small, springy "finger" that rides in the slot on the underside of the large stainless steel rod that is the cutting slide. Look at the back of the head for the "finger" and its adjusting screw. We use an old one, and found that replacing the cutting wheel gave it new life. We've also found that simply asking our local glazier to let their best "free-break" person cut up a 24" square of 1/4" plate into 1" strips works fine. We usually have to throw away the 2" near the end of the break, but the guy takes making these perfect strips as a personal challenge, and 24 1"x24" strips currently costs us about $17. ---We had some luck in making glass knives with LKB glass. Also, the glass was cleaned with 95% alcohol. When good knives were breaking - moon, humidity, incantation - I stored the knives in a desiccator for up to two years. Made a lot of them at one sitting. ---A key (you probably already know) is to check for any glass shards on the pedestals, no vibrations, a slow break - even pressure, good score not near edge of glass. Also I used 40 degree knives, not 45. ---I have had some of the same problems that you talked about. Right now our knifemaker is doing a good job but that does not mean that the next time it is used it will give good knives. When ours is misbehaving, I usually spend a good part of the day making adjustments without any improvements. Then suddenly and surprisingly, it gives good knives again. This state may remain for a few days or months, if I am lucky. ---Coincidentally I had a visit from a salesperson recently and I mentioned this to him and he told me about a SymParter that comes from Pelco. It is an attachment that goes on the knifemaker to enhance its performance. You might talk to the Ted Pella people about that. ---I think glass does make a difference and I have heard good things about Pella glass. We purchase ours from Mager Scientific, Inc., P.O.Box 160, Dexter, MI. 48130. I was planning to try Pella glass to alleviate our problem but since we both have the same problems with different brands of glass, the problem is probably not the glass. ---The old LKB glass is available from Ted Pella, and it really makes a difference. Has your knifemaker had a good tuneup lately (scoring wheel, clean slides of the head, caliper-measurement of the squares, fresh dampening pad)?? Careful attention to all of the above can make a huge difference in knife quality. I recently put in a nice fresh scoring wheel and carefully tweaked the scoring pressure with a great increase in knife yield (and consistency). Caliper-measure your squares!!
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I need to image fluorescently labelled live muscle cells in the living mouse. Is this possible to do? If so, what are the possible imaging technologies to accomplish this?
MRI microscopy? Optical tomography? Confocal?
Any input to get me started would be greatly appreciated. Thanks so much in advance.
Marc
Marc C. Brande, M.S. SD3D Email List:3D Imaging San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu 3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830
Earlier this year I solicited opinions about installing a 4.7 T NMR in the vicinity of an EM lab. Below is the upshot of that installation and a request from the NMR lab director for data on EM specs. Anyone who can be helpful should respond
Thanks } } We've now completed our move and turned the magnet on, with no observable } effect on EM performance (measured to 5.6 Angstrom resolution). On both } the SEM and TEM, pre and post images were obtained, as well as magnetic } field values measured pre, post, and during magnet energization. Both } DC (with a Walker flux-gate magnetometer, Model FGM-3D1, 0.1-2000 mG } range, DC-100 Hz response (200 mG and 2000 mG scales; DC-50 Hz on 20 mG } scale), NIST calibrated and certified) and AC (Walker ELF-45D, } 0.1-199.9 mG range, 30-300 Hz response) magnetic fields were measured. } } AC fields were 0.1-0.3 mG, and constant within that range, both before } and after energization of our 4.7T (47 kG) passively shielded magnet. } The earth's DC field in the hallway just outside the EM lab was about } 400 mG (305 mG horizontal and 265 mG vertical) and varied by about 5 mG } with time (several days). When the magnet was energized, a change of about } 12 mG was noted (17 mG had been predicted). Changes in varous locations } in the EM labs were 5-10 mG. The room was likely partially shielded by } structural metal and equipment (including the EM instruments) in the rooms. } The EM instruments themselves produce DC fields of from 100-1000 mG or more } in the vicinity of the columns, where the focusing lens are located. The } SEM had magnets on an ion getter pump within a foot of the top of the } column which produced a DC field at the column of over 2000 mG. } } It is obvious the 5-12 mG stray DC field produced at the EM site by our } NMR magnet is below the limits needed to cause problems for EM performance. } Since the earth's DC magnetic field varies considerably with location } (range at least 400-600 mG, and 150-300 mG horizontal, in the continental } U.S.) and somewhat less with time, it is likely that EM instruments can } compensate for stray, stable DC fields on the order of 100 mG or more. } Anecdotal reports indicate that this capacity may be as much as 300-500 mG } or more. The DC fields we measured near the EM columns corroborate these } anecdotal reports. } } I would appreciate receiving, by fax or e-mail, any information on AC and } (especially) DC field specifications that you might have for EM instruments. } Please include the manufacturer, model number, type (e.g., SEM, TEM), and } age of the instrument, as well as maximum magnification and accelerating } voltage range. A faxed or mailed copy of the original manufacturer } spec sheet would be most helpful. } } } } Richard W. Briggs, Ph.D. } Dept. of Radiology, Univ. of Florida } Box 100374, J.H. Miller Health Center } Gainesville, FL 32610 U.S.A. } tel: (904) 395-0680 ext 54279 } fax: (904) 395-0279 } e-mail: rbriggs-at-ufnmr1.health.ufl.edu } } -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- -at- Greg Erdos Phone: 904-392-1295 -at- -at- Scientific Director, -at- -at- ICBR Electron Microscopy Core Lab -at- -at- 218 Carr Hall Fax 904-846-0251 -at- -at- University of Florida E-mail: gwe-at-biotech.ufl.edu -at- -at- Gainesville, FL 32611 -at- -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Those of you who are involved with problems involving mechanical vibrations and magnetic fields might find it helpful to contact Wayne Vogen, President of VEC Inc. (Ph: 510-339-8719). He specializes in measuring these troublesome phenomena, and in finding methods for overcoming them, especially in EM laboratories. I've seen him in action a couple of times, and have been favourably impressed with his expertiese.
I wonder if any one knows the address of Epoxy Technology. I want to buy some Epo-Tek 353 ND epoxy for my XTEM samples but don't know the address of the company.
Thanks for help
Xinyang
Dept. of Materials Engineering University of Wollongong Australia
-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --
Martin Newman asked about "reliable TEM grid stainers".
The SPI Stain 'n Wash Grid Staining system is made primarily of glass (as opposed to plastic) and is designed to use a very minimal volume (e.g. 2-6 ml). It is usable for most heavy metal post-staining techniques (thin sections) as well as immunochemical labeling or enzyme localization techniques. Since one picture is still worth 10,000 words, I would refer you to our electronic catalog on the WWW site given below. Click "new products" and then "Stain 'n Wash".
I am told (by some of our customers) that the smaller than usual volume needed results in meaningful cost savings when expensive reagents are involved. One hundred grids can be processed at one time.
And any feed back I have ever received from customers has been quite positive.
Chuck
====================================================== Charles A. Garber, Ph. D. Ph: 1-(800)-2424-SPI President 1-(610)-436-5400 SPI SUPPLIES/Structure Probe, Inc. FAX: 1-(610) 436-5755 PO BOX 656 West Chester, PA 19381-0656 USA
e-mail: GVKM07A-at-prodigy.com [Direct for C. Garber] SpiSupp-at-aol.com [SPI Customer Service e-mail box]
I wonder if any one knows the address of Epoxy Technology. I want to buy some Epo-Tek 353 ND epoxy for my XTEM samples but don't know the address of the company.
Thanks for help
Xinyang
Dept. of Materials Engineering University of Wollongong Australia
The following message is a JOB POSTING for a SEM postion. Interested candidates should send a resume or direct inquiries to Jeff Kingsley by e-mail at jkingsley-at-cea.com, by FAX at 415 369-7921 or mail to 301 Chesapeake Drive, Redwood City, CA 94063. ----- Forwarded message follows -----
Return-path: {jkingsle-at-cea.com} Received: from cea.com by ENH.NIST.GOV (PMDF V5.0-4 #10952) id {01HU5GMSDXWG00QLOK-at-ENH.NIST.GOV} for phelps-at-ENH.NIST.GOV; Wed, 16 Aug 1995 18:57:18 -0400 (EDT) Received: from Connect2 Message Router by cea.com via Connect2-SMTP 4.00; Wed, 16 Aug 1995 15:58:46 -0700
Here is the job posting. Thank you for posting this on the listserv.
Charles Evans & Associates has an immediate opening for an experienced FE- SEM analyst in our Redwood City, California facility.
Charles Evans & Associates is a leading commercial analytical services laboratory specializing in surface analysis and materials characterization. Charles Evans & Associates has a central laboratory located in Redwood City, California, and associated laboratories and representatives world-wide. Currently the laboratory has a staff of more than 75 personnel and over 20 analytical instruments including SIMS, TOF-SIMS, AES, XPS, FE-SEM, SEM/EDS, RBS, TXRF, GDMS, AFM and FTIR.
The current opening is involved primarily with the preparation, FE-SEM imaging and interpretation of cross sections of semiconductor devices. The ideal candidate has 3-5 years of direct experience with semiconductor cross sectioning and FE-SEM imaging. The candidate must also have verbal and written communications skills suitable for customer interaction and report generation. Interested candidates should send a resume or direct inquiries to
Charles Evans & Associates is an Affirmative Action/Equal Opportunity Employer
The Education Committee of MSA has an extensive collection of VHS tapes on microscopy. For the past 10 years the tutorial sessions at the MSA annual meeting have been videotaped and are the backbone of the collection. However, videos have been obtained from other sources as well. The tapes will soon be available for sale at a modest cost. For a list of tapes or more information on acquiring them contact the Chair of the Education Committee:
JoAn Hudson. She can be reached at HJoan-at-clemson.edu
of check out the MSA Web Site
Jay Jerome ************************************************************** * aka: W. Gray Jerome * * Dept. of Pathology * * Bowman Gray School of Medicine of Wake Forest University * * Medical Center Blvd * * Winston-Salem, NC 27157-1092 * * 910-716-4972 * * jjerome-at-isnet.is.wfu.edu * **************************************************************
On Wed, 16 Aug 1995, Trevor Sewell wrote:
} Dear All, } } We would like to build up a library of training videos on aspects of } microscopy. Does anyone know any good ones? I would also appreciate } information on their current availability. } } Many Thanks } } Trevor Sewell } EM Unit } University of Cape Town } }
=========================================================== Rassie van Zyl INFRUITEC EM Unit Tel: 021-8839090 PRIVATE BAG X5013 Fax: 021-8838669 STELLENBOSCH Internet: rassie-at-infruit.agric.za 7599 ==============================================================
The women broke from a tangled embrace to answe the phone. When she hung up, her companion asked who it was. "Mu husband," He was calling to say that he would be late because he was bowling with you.
Please take me off the list (temporarily). Otherwise I will have 700 messages clogging my e-mail box when I return from vacation. Thank you for this courtesy. I'll resubscribe in the fall.
In the next issue of our newsletter "Microscopy Today" we offer you free listings for either Used Equipment For Sale or Used Equipment Wanted. Provide your CONCISE copy no later than 1 September by eMail or FAX (608-836-1969. And - should you not be the person responsible for purchasing used equipment at your organization, kindly supply us the proper name and address and we will see that he/she gets a no charge copy. Regards to all - Don Grimes, Microscopy Today
I have received a number of encouraging responses to my previous email about exploiting the web for teaching purposes. Exploring the web a little myself this morning, it is clear that many people are developing material which can be used, and I also talked to a few people as MSA who are interested.
The Web is an ideal tool for a distributed development, where if enough people contribute the effort becomes reasonable; developing a full WWW EM course by yourself is manyears of work. Let me float the idea of different people contributing small sections on different techniques. (There is already some of this out there, but I am going to email people directly to check that they are happy with their links being commonly known.) Some possible areas are: 1) Basic electron optics 2) Sample preparation 3) Bright Field/Dark Field 4) Image Contrast from dislocations 5).......
These should be at the level for undergraduates, with additional information available (or links) for graduate students.
There is also the question of small icons to keep interest and add a little color. (Icons need someone with artistic flair.)
Please send me an email if you are interested in contributing something, either in the future or a link that you already have. I will start a central clearing house and we can talk further by email (off the listserver).
} 5)....... I suggest Electron diffraction } } These should be at the level for undergraduates, with additional } information available (or links) for graduate students. } I think I can handle this; however, I will not be able to include CBED.
} There is also the question of small icons to keep interest and add } a little color. (Icons need someone with artistic flair.) } Someone else will have to do my icon.
} Please send me an email if you are interested in contributing something, } either in the future or a link that you already have.
} } I am interested in opinions concerning the relative merits } of keeping an EDS HV bias on continiously, as opposed to } shutting it off after every use. In other words, is there } any advantage wrt to stability of the electronics or } energy calibration to keeping it on? } Dear HHXS, One problem could be that, since the detector is more succeptible to radiation damage when the bias is on, unless your detector is very well shielded, you will damage it if you leave the bias on. Of course, if your instrument is dedicated to EDS--as opposed to a general-purpose 'scope with an EDS used occasionally--there will not be any extra damage. Yours, Bill Tivol
But how long have you been using them? We used 230MB internal drives but have found them incredibly sensitive to dust (presumably, because they are internal drives, the Mac ventilation system continually drags air, and dust, through them). After two years, everybody who used them has lost data.
Michael A. O'Keefe Acting Head, NCEM --------------------------------------
On Mon, 14 Aug 1995 wise-at-vaxa.cis.uwosh.edu wrote:
} To all, } } I have seen discussion of several of the various storage formats } for archiving electronic images--i.e. read/write CD, magneto optical, } tapes, etc. What are the collective thoughts on 3.5" cartridges (such as } Syquest)? They can hold up to 270 Mb and would seem to be a cheap (~$65) } alternative that can be read by any machine with a 3.5" drive. } } Bob } } } Robert R. Wise, PhD } Director, UWO Electron Microscope Facility } Department of Biology } University of Wisconsin Oshkosh } Oshkosh, WI 54901 } (414) 424-3404 } (414) 424-1101 fax } wise-at-vaxa.cis.uwosh.edu } } }
To add to Jay Jerome's info on the MSA Video Tape Library You can get a listing of all the tapes in the current library on the WWW. Following the hot links from the following site to the MSA Home Page.
http://www.amc.anl.gov
Go to the section on Microscopy Societies, then look for the Video Tape library document.
Anyone want a fully functional ancient Siemens 101 TEM for free (you pay costs of removal and shipping)? Lots of spare parts for anyone still running one of these instruments. Contact me directly ASAP or it will be removed as scrap.
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility 3 Tucker Hall University of Missouri Columbia, MO 65211 (314)-882-4712 (voice) (314)-882-0123 (fax)
The first one we purchased was when they first were selling the 230 instead of the 128, I think in the late spring of 1994. The first one we received, a Fujitsu, was defective, but its replacement has worked fine since then, over a year. We have lots of dust/soot in the Bronx, but these external drives seem to work ok. Somebody had a disk with a loose sticker on it so it got wedged int he drive; when I opened the drive (an external) to get it out, dust build up did not seem nearly as bad as the dust inside the computers themselves (I had to swap cards a few weeks ago and the dust on the motherboards looked like that under Duchamp's bed). We now also own two Olympus models. Other people around the University have these drives too, and I have heard no complaints about malfunction due to dust. -Michael
On 21 Aug 1995, Michael OKeefe wrote: } Reply to: Michael Cammer, Robert R. Wise } } But how long have you been using them? We used 230MB internal drives but have found them incredibly sensitive to dust (presumably, } because they are internal drives, the Mac ventilation system continually drags air, and dust, through them). After two years, } everybody who used them has lost data.
Dear Fred- Yes, Spurrs can be polymerized in a microwave. There are a series of papers by Beverly Giammara on the subject. I'll send you a bibliography if you like, and also copy this reply to Beverly so she can respond to you directly. The procedure is done in flat Silastic embedding molds (BEEM capsules will melt) in about 30 minutes. We recommend use of a laboratory microwave with a vent interlock system to avoid the possbility of your breathing the fumes from the resin. Best regards, Steven Slap
Dear Trevor- There is a whole library of videos available from the MSA Business Office. Contact Larry Maser at mmaser-at-mbl.edu and he'll send you a catalog. We have found them very useful. Steven Slap
Dear Peter, } } I am curious about this reply. Why do you think radiation damage } would be higher with the bias on? } I was told this by Kevex, and I accepted it without much thought. Now that you bring it up, it would seem that the kinds of damage brought about by a 1.2 MeV e- beam would occur independently of whether the bias was on. There might be some affect on recombination, but this seems unlikely with the 500 volt setting for the bias. Possibly I just misunderstood Kevex. Maybe some- one from Kevex will comment. Yours, Bill Tivol
To the helpful, I'm trying to do platinum/carbon replication of cellulose membranes containig 10 nm pores. We have to dissolve the membranes after shadowing-where lies the problem. My partner heard of "melange chromic acid" at a thesis defense which is supposedly bichromate dissolved in sulfuric acid (what concentration?). She can't find bichromate in the catalogs,so we need advice. Is there an alternative solution which will dissolve cellulose membranes and how long does it take? Any suggesstions or references will be greatly appreciated.
I'm tying to do platinum-carbon replica's of cellulose membranes containig 10 nm pore's and we have to dissolve away the membranes-where lies the problem. My partner heard of "melange chromic acid" at a thesis defense which is supposed to be bichromate dissolved in sulfuric acid (what concentration?). She could not locate bichromate in any of the catalogs, is there an alternative solution which will dissolve cellulose membranes and how long does it take? Any suggestions or references will be greatly appreciated.
} My partner heard of "melange } chromic acid" at a thesis defense which is supposedly bichromate } dissolved in sulfuric acid (what concentration?). She can't find bichromate } in the catalogs,so we need advice.
Dear Michael, Try potassium dichromate. The solution is the same as chromerge (except possibly for concentration). The best concentration depends on the use, and I'd guess that dissolving membranes does not require the high-test. For dissolving silver from photo tanks I pour ~ 25 cm^3 of potassium dichromate into ~1/2 tank (2 l) hot water. I add concentrated H2SO4 (~50 ml) and top off with hot water. The residue dissolves almost instantly. Be careful adding the acid to the water--NEVER ADD WATER TO CONCENTRATED SULFURIC ACID. I'd suggest trying ~1% dichromate in 1 M H2SO4 to start and changing the concentrations if necessary. BTW, a stock solution of dichromate in 50% H2SO4 will be stable indefinitely, so you may want to make this up first and mix with the appropriate amounts of water and H2SO4 to get the various concentrations you need. This mix is an oxidising acid! It will dissolve clothing (and, possibly fingers) and will react explosively with organic materials--another reason to start with low concentrations. Good luck & be careful. Yours, Bill Tivol
I do not know if this is what you are looking for, but in our hands it works fine. Please be careful when you prepare the mixture:
Take a flask of 250 ml put in 100 ml of 10% sulfuric acid (H2SO4) and put it on ice. carefully and slowly add 25 grams of chromic oxide (CrO3). After dissolving all the chromic acid, we use this mixture to etch away our material in a dilution 1part melange and 3 parts of water, and we let it stand overnight before cleaning it with water carefully. But you can try higher concentrations and play around with time. If you use higher concentrations there might be a chance that you find crystals in your replica afterwards. But give it a try. In our experiments on plant material we also use in addition after careful washing 4% household bleach(unthickened). Just give it a try. If there are problems please let me know to see if I can be of some kind of help
I'm tying to do platinum-carbon replica's of cellulose membranes containig 10 nm pore's and we have to dissolve away the membranes-where lies the problem. My partner heard of "melange chromic acid" at a thesis defense which is supposed to be bichromate dissolved in sulfuric acid (what concentration?). She could not locate bichromate in any of the catalogs, is there an alternative solution which will dissolve cellulose membranes and how long does it take? Any suggestions or references will be greatly appreciated.
Michael Delannoy Microscopy Facility JHUMI
- - - - - - - - - - - - End of Original Message - - - - - - - - - - - -
Henk Kieft Dept Plantcytology and morphology Wageningen Agricultural University Arboretumlaan 4 6703 BD Wageningen The Netherlands tel: (+)31 8370 84863 fax: (+)31 8370 85005 E-mail: henk.kieft-at-algem.pcm.wau.nl
-- --------------------------------------------------------------------------- | Mary Russell EMail mary-at-debenres.demon.co.uk | ---------------------------------------------------------------------------
Hey Piggies, I just got my E-mail up and running and have been reading these old messages about the "game". All I know is that I had a great time playing this year, and even had fun at the pig party (except for the "load dumping incident"). Anyway I'll be back next year, as I hope everyone else will (even Meeker), and would be proud to pit the pigs against any team as long as we can really pitch with an impartial ump. Whatever the score, our team never gave up (even against the Raiders), which I really didn't experience with the 90-92 team (probably because we were to tanked to even walk!). Anyway, until next season---} SUUUU WEEEE!
You should be looking for dichromate in the catalogue. I use chromic acid for cleaning replicas made from all sorts of food preparations containing proteins and fat.
Dissolve 5g of sodium dichromate in 5ml water. To this solution carefully add, with stirring, 100ml of concentrated sulphuric acid.
The replicas will require about 24h for cleaning.
Hope this helps.
Tony McKenna Food Science New Zealand Dairy Research Institute
You can also use 1% sodium hypochlorite (bleach) to clean tissue off of replicas. I use it when doing quick-freeze, deep-etchin gand rotary shadowing. The tissue I have used is eggs, btu the egg jelly of the Xenopus is pretty tough and still dissolved in bleach for 1-3 hrs.
Eric A. Rosen Department of Biological Sciences Califrornia State University, Fullerton Fullerton, CA 92624-9480
-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --
On August 22, Michael Delannoy wrote about problems with the platinum/carbon replication of cellulose and imaging 10 nm pores.
Over the years, we have encountered this kind of a problem, that is, with organic material "sticking" to the replica, preventing one from being able to "see" through it by TEM. We encounter this kind of problem not just for "ordinary" Pt/C replication situations, but also, for removing organic type residues from freeze fracture "replicas". A more common situation is the removal of polymer latex particles that tend to "stick" in a freeze fracture situation.
We have found that the following approach offers advantages over any of the "liquid" kinds of approaches, since the surface tension forces of the liquid tend to disrupt the fine texture and pores one is trying to resolve:
a) After replication with Pt/C [sometimes it is necessary to use only Pt, in extreme cases], the "replica" is then "backed" with a layer of SiO2, deposited by the evaporation of SiO from a tungsten basket. If in a freeze fracture device, one would need another set of "posts" to follow the Pt/C with the SiO coating.
b) One then does their best to remove the organics however else they would try doing this, e.g. stripping, etc.
c) What is left is put into a plasma etcher (of the type made by SPI Supplies would be our subjective preference, but ones made by others, such as Fisons would probably work as well), and using oxygen only, and typically for periods of time of less than one minute (ten seconds might be all that is needed), all remaining organics are removed. The reason for the "backing" with SiO2 (or who knows, maybe there is some SiO as well) is that the oxygen plasma will not react with this second layer, giving the replica coherence it would not otherwise have. The Si is low enough in atomic number as not to result in any meaningful loss of contrast.
d) One way or another, the replica is put onto a TEM grid and voila!, and with a bit of luck, one sees what they are looking to see. Since the grain size of the Pt when done as Pt/C is a bit under 1nm, the resolution of the shadowing material should be good enough to at least get a sense about 10 nm pores.
If it does not work the first time, don't worry, it does not work the first time for anyone! Indeed if you think it did work the first time, then you probably did something wrong. In any case, it is a "delicate" procedure and you want to spend more than the normal amount of time "validating" the procedure for your kinds of samples. Putting it another way, you should, for this method, try to be your own worst "devil's advocate" and make sure you have the answers to the questions that will invariably come up.
Disclosure: SPI Supplies manufactures the Plasma Prep II plasma etcher and is also a supplier of the silicon monoxide "chunks" used in this procedure. Best results will be obtained by using tungsten baskets instead of boats, which means you want silicon monoxide "chunks" and not a powder.
This is the first time I have ever really "published" this bit of secret "art", for removing the last vestiges of organics from a replica. I would appreciate any feed back on your successes or failures and/or ideas for improvements to the method.
Chuck
====================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: GVKM07A-at-prodigy.com West Chester, PA 19381-0656 USA Customer Service: SpiSupp-at-aol.com
I'm looking for details on the 34th Annual Conference of the EM Society of Southern Africa. I have an email address (which I got off the net) but it doesn't seem to be current (not recognisable at any rate). Although I also have a postal address for Prof. M.E. Lee at the EM Unit in Sovenga SA., I would prefer to contact him by e-mail or fax.
The only details that I have on the EMSSA-95 meeting are as follows:
34th Annual Conference of the Electron Microscopy Society of Southern Africa (EMSSA 95) Nov. 28 - Dec. 1 , 1995 Aventura Resort, Warmbaths, Northern Transvaal Province, South Africa Contact: Prof ME Lee, EM Unit, University of the North, Private Bag X 1106, Sovenga 0727, South Africa. E-mail: qemssa95-at-unin1.north.ac.za
If it is pure cellulose you are going to remove why not try a 0.1% cellulase solution for 2-5 h. - In combination with pectinase it works very well with plant material.
The information published gave the contact as Prof. Mike Lee, EM Unit, Univ. of the North, Private Bag X1106, Sovenga 0727, South Africa; e-mail: qemssa95-at-uninl.north.ac.za. This information was published in Microscopy Today. Steven Slap
Don- There are at least three formulations of Quetol. The references are: Quetol 653: Kushida, H, J Elec Mic, 29/2, 193, 1980 Quetol 651: Fujita et al, J Elec Mic, 23/2, 165, 1977 Quetol 812: Kushida, H, J Elec Mic, 32/1, 65, 1983 They are all made in Japan. Steven Slap
I read Dr. Charles A. Garber's reply to the above subject with interset. I have not done plasma etching myself and would appreciate some more details.
Could Dr. Garber provide details of how the Pt/C-SiO-organics was supported during etching; whether the organic face was up and how the clean replica was deposited onto a TEM grid. Many thanks.
Quetol 651 is dibutyl glycidyl ether of ethylene glycol. For your interest, DER 736 (additive to Spurr's) is dibutyl glycidyl ether of polyethylene glycol. I'm curious to know why you ask--I have experienced repeated problems with this resin in the last two years. Grace Kennedy
MicroWorld Resources and News is pleased to announce an e-mail version of it's WWW newsletter. It will include a section on what is new on the WWW, a list of the job openings and resumes that are on-line at MicroWorld, microscopy news and press releases, and a list of upcoming meetings. If you would like to submit an item, including information about your WWW site, please send it to me. To receive the newsletter by e-mail, return this message back to me. Inclusion of your real name and affliation is optional. Please take care NOT to post the return to this listserver.
All comments are welcomed.
Susanne Pignolet Brandom, Ph.D. MicroWorld Resources and News http://mwrn.ms.wwa.com/topfile.htm spb-at-wwa.com or spignole-at-ix.netcom.com
Could the colleague who posted a note about glycogen histochemistry a few month ago send me at {fermin-at-tmc.tulane.edu} the receipe/references for demonstrating the various configurations of glycogen in cells. A colleague here at Tulane Medical school wants to look a human muscle glycogen. Thanks.
I'm looking for parts and schematics for a Mikros VE10 evaporator which has come into my possession but needs work. Any suggestions of a source of the aforementioned would be appreciated. You can reply to me directly thanks steve
---------------------------------------------------------- Dr. Steven Barlow EM Facility/Biology Dept. San Diego State University San Diego CA 92182-0057 phone: (619) 594-4523 fax: (619) 594-5676 email to sbarlow-at-sunstroke.sdsu.edu
Can anyone help me with some lectin labeling problems I am having. I am trying to label the tunicate egg ECM with a variety of TRITC conjugated lectins purchased from EY Labs. I am having a problem with the controls. The eggs are fixed in 3%Formaldehyde, 0/5% osmium and embedded in LR white resin. 15 one micron sections are cut and stuck to coverslips with which I immerse in the solution containing the lectins and competative sugars. The controls are prepared as follows: 1) the lectins are incubated in the competitive sugar that is specific for it for 60 minutes at room temp, ranging in concentration from .1mM to 1M and the sections are pre-incubated in the sugar fro 30 minutes prior to adding the lectin/sugar mixture. These are then incubated 60 minutes with agitation and then washed 45 minutes in appropriate buffer. The problem is the controls I think almost look like the experimentals. The concentration of the lectin in the solution is 25 micrograms/ mL.
What am Idoing wrong if anything and does anyone have any suggestions? I need to get this done by the August 31.
Cheers,
Eric A. Rosen Department of Biology California State University Fullerton, CA 92634-9480 (714) 449- 5291 (714) 773-3426 xerosen-at-fullerton.edu
-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --
Ann-fook Yang (Aug. 24) asked for more clarification on the plasma etching method:
There are two situations, each slightly different but sort of related:
a) Surface replications such as from the surface of a piece of paper with exposed microfibers, so that when the Pt/C (or just Pt) is shadowed onto the surface, a stripping agent is used (we prefer polyacrylic acid, 1 or 2% aqueous), and when it is hardened into a tough film and stripped off, enough of the fine fibrils are stripped off as well, making it difficult (or impossible) to see through the replica.
Our variation on the theme is to "back" the Pt/C, instead of with an extra layer of carbon, we apply a layer of SiO2. From that point on, one does the stripping, dissolves away the PAA leaving the "replica" (but backed with SiO2) floating on the water surface. In this instance, a piece can be picked up on a grid of your choice, and THEN the entire grid is "etched" in a plasma etcher (not more than 100 watts power) for maybe not more than 10 seconds (if using pure oxygen). The oxygen plasma will etch away the fine fibrils clinging to the replica, but will not "etch" the SiO2 support film (but if the support film was carbon, it would eat away the carbon as well, and then there would be no sample left). The exact etching times will vary depending on the etching unit being used. We recommend a chamber geometry that features a "manifold"design, to increase the etch rate and minimize the etch time. If you can not guess which one (uniquely, so far as I know) has that kind of design, e-mail me and I will tell you.
b) For freeze fracturing and etching, what I was thinking about was the kind of sample such as suspended polymer latex (emulsion particles) or even certain liquids with macromolecular viscosity modifiers (e.g. certain heavy greases, motor oils with additives, etc), and again there is this problem of having too much organic material clinging to the replica. So if it is "backed" with SiO2 instead of C, it can be "cleaned up" the same way, rendering otherwise non-useable replicas, useable.
Common sense would dictate that the side to be etched should be "up" but the reality is, from our experience, it does not seem to matter because of the isotropic nature of the etching. Being primarily a materials science person, I was responding from the perspective of materials science applications, momentarily forgetting that there was a life science perspective as well (e.g the clean up of biological samples). Sorry about that.
It seems that the dry etching method has advantages when the material to be removed will not respond to the chemical etchants or when fine structures are involved for which surface tension effects could produce artifacts. For the typical life science situations that apparently most people are working with, I can not say that the dry etch method would have advantages except possibly in the two types of instances where noted.
I am trying to accumulate some kind of a list of publications where dry plasma etching has been used to clean up replicas, so I would appreciate by e-mail any such references. Thanks in advance.
Chuck
====================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: GVKM07A-at-prodigy.com West Chester, PA 19381-0656 USA Customer Service: SpiSupp-at-aol.com
} Does anyone know where to buy an anti-static gun for removing static charges } during microtoming? Many thanks in advance. } } } X. Zhang } } E-Mail: Xiao.Zhang-at-GEP.GE.COM } Phone: (518)475-5241 } FAX: (518)475-5969
You might try a product called Zerostat. It has a gun type grip that, when squeezed, distorts a piezo-electric crystal that discharges ions to temporarily neutralize static. They used to be available at record shops and from some of the EM suppliers. The most recent price I found in the Ted Pella catalog was $55 each.
These also work great for getting rid of static when weighing powders and pouring liquid embedding plastic components into plastic tri-pour beakers.
Department of Physics, University of Wisconsin Milwaukee
Title: Electron Microscopist (Instrumentation Specialist) Appointment: Academic Staff 50%, Fixed Term Salary Range: Salary is competitive Application Deadline: September 8, 1995 (postmark) Starting Date: September 25, 1995 (approximate)
Application Procedure: Send a letter of application, resume and names addresses and telephone numbers of three references to: Prof. Marija Gajdardziska Department of Physics University of Wisconsin Milwaukee P. O. Box 413 Milwaukee WI 53201 Fax (414) 229 5589
Responsibilities: Daily operation, maintenance and user support of the laboratory for high resolution electron microscopy and the associated laboratory for specimen preparation. Duties include:
* Daily start-up of the instruments, alignment of the microscope, basic maintenance and troubleshooting, scheduling and coordination with manufacturers' service representatives; * User training and assistance in: basic operation, alignement, high resolution imaging, microanalysis, and specimen preparation; assistance in laboratory section of graduate course in electron microoscopy; * Preparation of specimen and operation of the microscope under scientific guidance of faculty for approved academic and industrial research projects; * Assistance in scheduling and billing for microscope time, purchasing of supplies for the microscopy laboratories, purchasing and installation of instruments in future laboratory expansions and/or renovations, establishment and implementation of records, regulations and procedures for effective laboratory functioning.
Qualifications: B.S. or B.A. in general science, physics or electronics with coursework and/or a minimum of 2 years experience in electron microscopy. The successful candidate will have:
* Detailed knowledge of the operating principles of transmission electron microscopes (TEM) with emphasis on physical sciences applications; * Demonstrated ability to operate, maintain and troubleshoot a TEM; * Demonstrated ability to train researchers how to use a TEM; * Working knowledge of vacuum systems, TEM specimen holders and specimen preparation; * Elementary knowledge of crystallography, solid state matter and computers; * Good communication skills for clear and efficient interaction with faculty, students, technicians and service engineers; * Experience in phase contrast imaging and diffractogram analysis is desirable but ability to learn new techniques of imaging, diffraction and spectroscopy is a must.
The Department of Physics and UWM are affirmative action, equal opportunity employers. The names of those applicants who have not requested that their identities be withheld and the names of all finalists will be released upon request. *******************************************************************************
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I4m setting up an endothelial capillary culture system for microscopy according to Cooke 1993. Does any one know where to find flat glass capillaries with an optical quality suitable for high res microscopy ? Any suggestions are welcome. Does any one have experience of such culture systems ?
============================================= Mikael Gustafsson MD, PhD Dept Med. Microbiology and Dept Internal Medicine, Cardiology section University Hospital of Linkoping S 581 85 LINKOPING SWEDEN
Have you considered the Omega drives? The Zip drive has 100 meg cartridges and is rather inexpensive ($200 for drive and ~$20/cartridge). Also, they're about come out with their new Jazz drive, which will have 1 Gig cartridges and, they claim, to have performance close to HDs.
Subject: Time: 5:25 PM OFFICE MEMO RE:IUMAS in Australia Date: 8/28/95
The meeting you inquired about is the First meeting of the International Union of Microbeam Analysis Societies (IUMAS). It will be held at the University of Sydney, Sydney, NSW Australi from Feb 5 to 9, 1996. It will be preceeded by several workshops, and will be held in conjunction with the 14th Conference of the Australian E.M. Society and the 9th. conference of the Light Microscopy Society. Information can be found on WWW at: http://www.bio.uts.edu.au./em.html; or can be obtained from: ACEM 14-microCosmopolitan, E.M. Unit, University of Sydney, N.S.W. 2006, Australia Ph: 61-2-351-2351 and Fx: 61-2-552-1967
Chromic acid has been used for many years as a cleaning agent for glassware in chemical laboratories. According to the 35th. Ed. of the Hancbook of Chemistry & Physics (p. 2990), a solution appropriate for this purpose is prepared by pouring (slowly, carefully, and with stirring) one litre of concentrated sulfuric acid into 35 ml of a saturated aqueous solution of sodium dichromate (Na2Cr2O7).
} Can anyone give me information about the Australian conference to } be held in Feb 1996? } regards } mark
The next Australian EM Conference ACEM-14 will be held at the University of Sydney 5-9th February 1996. This will be a joint meeting with the 1st International Conference of IUMAS (International Union of Microbeam Analysis Societies). The conference is hosted by the Australian Society for Electron Microscopy and the Microscopical Society of Australia.
The contact address for registration forms, fees and enquiries is: ACEM-14 c/- ACTS, GPO Box 2200 Canberra, ACT 2601. Tel (06) 257 3299 Fax: (06) 257 3256. Sorry - Don't know if they have an e-mail address.
This recent thread on preparing and using chromic acid solutions for various cleaning purposes brings an important point to mind. The qualities that make chromic acid such a good cleaning agent also make it extremely hazardous to prepare, use, and store. Added to this, it is very difficult to dispose of properly. Our environmental safety personelle absolutely HATE it. For the past several years, they have been encouraging laboratories to switch to more modern "equally effective" means for cleaning. Has anyone else experienced this pressure to switch, and has a suitable alternative been found?
-=W.L. Steffens=- Department of Veterinary Pathology College of Veterinary Medicine University of Georgia
Thanks to Jim Heuer for the info about the Static Master available from VWR. The Zero Stat guns are no longer available from any of the EM suppliers as far as I can tell, and I've been looking for a substitute source, or another item. Martin B. Garment Ophthalmology and Visual Sciences 1300 University Ave. Rm. 6687 Madison WI 53706 Voice (608) 262-9596 Fax (608) 262-0479 Email - mgarment-at-facstaff.wisc.edu
I have been using a device for the last 20 years or so to eliminate static charges during microtomy. This device, the staticmaster is available from many large photography supply houses. Hilton Mollenhauer introduced me to it, and I have yet to find a better remedy to the static problem. This device is a small cartridge, available in 3 sizes (I use the smallest). It is meant to attach to a special camel-hair brush for removing dust particles from negatives prior to printing. The cartridge itself consists of an element containing a polonium source, an alpha emitter, and is effective for about 5 years. I mount the cartridge (w/ double-stick tape) as near as possible to the knife edge on the microtome...the result is no static attraction problems. Of course, since its a radiation source, it must be disposed of properly when exhausted. It is available from: NRD Inc., Grand Island, NY 14072 (800-525-8076). I have no interest in this company, I just like the product.
-=W.L. Steffens=- Department of Veterinary Pathology College of Veterinary Medicine University of Georgia
Integrated Microscopy Resource (IMR) a Biomedical Research Resource
at:
The Wisconsin Center 702 Langdon Street Madison, WI 53706
PROGRAM
FRIDAY (EVENING), SEPTEMBER 29, 1995 6:00 - 7:55 OPENING RECEPTION 8:00 - 8:45 Ken Jacobson, Univ. N. Carolina-Chapel Hill "Imaging the Traction Forces Exerted by Locomoting Fish Keratocytes" 8:50 - 9:35 John Sedat, Univ. California-San Francisco "Multi-dimensional Optical Microscopy and EM Tomography: New Approaches and Results" 9:40 - 10:25 Edward Salmon, Univ. N. Carolina-Chapel Hill "Multi-mode Light Microscopy of Microtubule Assembly Dynamics and Motor Proteins in Mitosis and Related Movements"
SATURDAY, SEPTEMBER 30, 1995 8:30 - 9:15 D. Lansing Taylor, Carnegie-Mellon University "Molecular Dynamics of Force Generation during Cytokinesis" 9:20 - 10:05 Jeff Lichtman, Washington University "Imaging Synaptic Competition in Living Animals" 10:10 - 10:40 COFFEE BREAK 10:45 - 11:30 John White, Univ. Wisconsin-Madison "Evaluation of Multiple-Photon Excitation Fluorescence Imaging for In Vivo Studies" 11:35 - 12:20 Steven Smith, Stanford University "Dynamics of Hippocampal Dendrite Growth and Synaptogenesis" 12:25 - 1:25 LUNCH (on your own) 1:30 - 2:15 Gary Borisy, Univ. Wisconsin-Madison "Using Correlative Microscopy to Study the Dynamics of the Cytoskeleton" 2:20 - 3:05 Ralph Albrecht, Univ. Wisconsin-Madison "Using Correlative Microscopy to Follow Cell Surface Receptor Ligand Complexes and Associated Subjacent Intracellular Events" 3:10 - 3:55 Paul Bridgman, Washington University-St. Louis "Multiple Microscopic Techniques Applied to Growth Cone Intrapodia Fixed During Live Observation"
5:00 - 6:00 EXHIBITOR'S SOCIAL 6:00 - 8:00 BUFFET DINNER
SUNDAY (MORINING), OCTOBER 1, 1995 9:00 - 9:45 Kent McDonald, Univ. California-Berkley "Cryotechniques: New Hope for the Ultrastructurally Challenged 9:50 - 10:35 Stanley Erlandsen, Univ. Minnesota "Leukocyte Rolling and Transendothelial Migration:
Importance and Distribution of Cell Adhesion Molecules" 10:40 - 11:10 COFFEE BREAK 11:15 - 12:00 Hans Ris, Univ. Wisconsin-Madison "High-resolution FESEM Imaging of Internal Cell Structures after Epon Extraction from Sections: A New Approach to Correlative Ultrastructural and Immunocytochemical Studies" 12:05 - 12:50 Eric Henderson, Iowa State University "Biological AFM and Molecular Force Detection"
FEES: General Registration $100.00 (On Site: $130.00) (Includes: Opening Reception, Social and Buffet Dinner, Coffee Breaks and Materials)
Local Registration $ 80.00 (On Site: $110.00) (Includes: Opening Reception, Social, Coffee Breaks and
Materials)
FOR ADDITIONAL INFORMATION (including abstracts) CONSULT OUR WEB SITE
http://www.bocklabs.wisc.edu/imr/imr.html
TO RECEIVE A BROCHURE AND REGISTRATION FORM SEND COMPLETE MAILING INFORMATION TO:
IMR Univ. Wisconsin-Madison 1675 Observatory Drive Madison, WI 53706
OR EMAIL: imradmin-at-calshp.cals.wisc.edu
#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#* Presentations will focus on biological problems for which a combination of microscopies [i.e. integrated microscopy] has been used. The speakers will demonstrate by example the power, potential and limitations of various microscopical techniques. The techniques which will be discussed include: DIC, Confocal, 2-Photon Excitation Imaging, SEM, TEM, Cryo-specimen Preparation, and AFM. #*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*
Following the symposium, the IMR will be conducting a 2-day workshop (Oct 2 and 3). We will be presenting lectures and provide "hands-on" experience for the following techniques: * 2-photon excitation imaging * 4D DIC imaging * Cryo-SEM * High pressure freezing * Reversible embeddment for SEM and TEM
Workshop attendence will be limited to 25 participants. A letter of application is required. Once accepted a fee of $150.00 will be due.
We need to purchase a microinjector system to label cultured fibroblasts while viewing on a Leica CLSM. Can anyone recommend one which has worked well for them and is not outrageously priced? Manufacturer, model and price please
Thanks
Sandra K. Masur Box 1183 fax: 212-289-5945 Mount Sinai School of Medicine phone: 212-241-6544 or 0089 NY NY 10029-6574 e-mail:masur-at-msvax.mssm.edu
My apologies to the list, but, I have misplaced the address for someone on the list server and need to get a hold of them. Could Greg Martin at Johns Hopkins School of Med please email me. Thank you
Mark Elliott UBC-Pulmonary Research Lab, Vancouver BC melliott-at-prl.pulmonary.ubc.ca
I need advice with a project: we are trying to evaluate bacteria which were grown on/in a porous matrix made up of alumina/silicon we want to examine the samples by TEM is there a method or plastic which could infiltrate the cells, and also be hard enough to allow thin (50nm) sectioning?
we have tried LR white (hard) but the matrix (very "brittle") splinters away from the resin when thin sections are cut, if we section thicker (100+ nm) sections .. they are too dense for the (100 kV) e-beam to penetrate
} Some time ago, I read an e-mail message that someone uses a SEM } printout port (to a dot matrix printer) to transfer data to a PC. } Currently, my Kevex Delta EDS system (PDP-11/TSX operating system) is } connected to a PC through TCP/IP for transfering images and data files. } But there is one type of data set in the Kevex system I can not save onto } the disk and only stays in the memory. I can not transfer it to the PC. } But I can print it out to a dot matrix printer (but not to data file) } through the Digital Dec computer. } I need your help on information how I can intercept the data to } the dot matrix printer and acquire it into my PC. Could some tell me what } kind of module or software I need in order to do this task? }
I have successfully transferred data by connecting the printer output cable to COM1 of my PC. It only required a 25 pin to 9 pin adapter. I run Kermit on the PC. Give a Log Session command with a filename then connect(C). On the Kevex side give the Print command. After the data have listed, hit Alt X on the PC and close session.
Recently Mike Rock posted: } I need advice with a project: } we are trying to evaluate bacteria which were grown on/in a porous } matrix made up of alumina/silicon } we want to examine the samples by TEM } is there a method or plastic which could infiltrate the cells, and also } be hard enough to allow thin (50nm) sectioning? } etc.
At Purdue we do some microtomy of hard materials (aluminum, steel, AlN,SiC) and we have used Struers Epofix resin for specimen mounting. This resin is expressly for mounting of relatively hard materials, although it sometimes adheres best with some silane coupling agent that I can't think of at the moment.
good luck,
Kirk
Kirk A. Rogers krogers-at-materials.ecn.purdue.edu 317-494-8751 office http://materials.ecn.purdue.edu/~krogers/ (coming soon) 317-494-1204 fax Purdue University, School of Materials Engineering, 1289 MSEE building, W. Lafayette, IN 47907-1289
} I was told of a device (a modified funnel with some special sieve in it?) } used to remove contaminating moisture from liquid N2. It may be commercially } available but I can't remember ever seeing anything like it. My colleague } could not be more specific.
We have two LN2 set-ups which may be relevant. Our Gatan cryo-trans- fer stage comes with a funnel with a sieve in it--Gatan may be able to give you the name of a supplier or to sell you one separately. The only problem with their funnel is (possibly) the size; its stem is ~1/4" o.d., so if you need to deliver large amounts of LN2, this won't do it. Our main LN2 system has liquid separators, so that only the liquid is sent down the pipe. These consist of cups with small holes in the bottom, so that the liquid falls through. We installed a home-made one on one fill line; we just took a plas- tic cup and punched holes in it. If you don't find a commercial supplier, and your shop can machine either perfluoro-polyethylene or polyethylene, have them make a cup and put a stainless steel screen in it. The screen mesh should be small enough to trap the ice, so it's pretty fine. Good luck. Yours, Bill Tivol
I am looking for a lab which is ASTM approved for testing oxygen index and absorbtion. Additionally, I need a porosity # 50psi test as well. I am looking for ASTM # D2863-77 and ASTM #D 570-81. If anyone has this information please send me a name and phone number where I may have these tests run. Any help will be very much appreciated. Thank you. JoAn Hudson-at-ces.clemson.edu
Received: from MAILQ by PNCRI-3 (Mercury 1.21); 1 Sep 95 00:48:07 GMT+1300 Return-path: {deschuyt-at-sbbio.be} Received: from date.palm.cri.nz by hrp2.palm.cri.nz (Mercury 1.21); 1 Sep 95 00:48:04 GMT+1300 Received: from hrp1.palm.cri.nz ([161.66.1.10]) by date.palm.cri.nz (8.6.9/8.6.9) with ESMTP id AAA25894 for {HRPRJB-at-hrp2.palm.cri.nz} ; Fri, 1 Sep 1995 00:49:40 +1200 Received: from PALMCRI/MAILQ by hrp1.palm.cri.nz (Mercury 1.21); 1 Sep 95 00:46:47 GMT+1300 Received: from MAILQ by PALMCRI (Mercury 1.21); 1 Sep 95 00:46:23 GMT+1300 Received: from aaem.amc.anl.gov by hrp1.palm.cri.nz (Mercury 1.21); 1 Sep 95 00:46:13 GMT+1300 Message-Id: {199508311236.OAA28502-at-gate.sbbio.be}
I am looking for a lab which is ASTM approved for testing oxygen index and absorbtion. Additionally, I need a porosity # 50psi test as well. I am looking for ASTM # D2863-77 and ASTM #D 570-81. If anyone has this information please send me a name and phone number where I may have these tests run. Any help will be very much appreciated. Thank you. JoAn Hudson-at-ces.clemson.edu
The September issue of Computer Shopper as a main feature article on CD-R's (Recordable CD's) discussing 6 currently availible units for under $2,000, as well as some very useful hints on actually recording CD media and software.
If your interested you can either hunt down the article or check out their web site WWW.ZIFF.COM which has a full search feature for topics found in all the computer related Ziff-Davis journal publications. (Access to the site is free, and registration to the site is also free.)